WorldWideScience

Sample records for chemical mutagens ems

  1. Mutagenic Effects of Some Chemical Agents in Wheat (Triticum aestivum L. em Thell)

    International Nuclear Information System (INIS)

    Water soaked wheat cv. NW 1014 seeds were treated with aqueous solutions (1 per cent) of 6 chemicals, namely, acid slurry, sodium carbonate, tri-sodium phosphate, sodium tri-polyphosphate, carboxy methylcellulose and sodium sulphate for 22h. Out of these chemicals, tri-sodium phosphate and carboxy methylcellulose recorded the highest mutation frequency (1.15 per cent), followed by sodium sulphate (1.00 per cent), sodium carbonate (0.85 per cent), sodium tri-poly phosphate (0.65 per cent) and acid slurry (0.0 per cent) in the M2 generation. The highest number of mutants was observed for late heading (47), followed by dwarf stature (36), white spike (7) and high tillering (6) in the M2 generation. On the basis of the number of high yielding mutants (M5), the mutagenic efficiency of sodium carbonate may be placed at the top rank, followed by sodium sulphate, tri-sodium phosphate, sodium tri-poly phosphate and carboxy methyl cellulose. One type of macro mutant (white spiked) was induced by tri-sodium phosphate whereas sodium carbonate generated 3 types of macro-mutants (large flag leaf, club shape spike and small grain ). The studies reveal that all the chemicals, except acid slurry, may be used as mutagenic agents in wheat. (author)

  2. Mutagenic efficiency of radiations and chemical mutagens in inducing viable mutations in rice

    International Nuclear Information System (INIS)

    Studies were undertaken to compare the effectiveness and efficiency of radiations (gamma rays and fast neutrons) and chemical mutagens (EMS and NMU) in inducing viable mutations in rice. Radiations were more effective than chemical mutagens, the most effective being fast neutrons. Mutagenic efficiency when estimated on the basis of lethality was higher for radiations but when based on sterility was higher for chemical mutagens. Fast neutrons, more effective than gamma rays, were less efficient. NMU was more effective but less efficient than EMS. (author)

  3. Induced mutations in chickpea (Cicer arietinum L.) I. comparative mutagenic effectiveness and efficiency of physical & chemical mutagens

    International Nuclear Information System (INIS)

    Mutagenic effectiveness usually means the rate of mutation as related to dose. Mutagenic efficiency refers to the mutation rate in relation to damage. Studies on comparative mutagenic effectiveness and efficiency of two physical (gamma rays and fast neutrons) and two chemical mutagens (NMU and EMS) on two desi (G 130 & H 214), one kabuli (C 104) and one green seeded (L 345) chickpea (Cicer arietinum L.) have been reported. The treatments included three doses each of gamma rays (400, 500 and 600 Gy) and fast neutrons (5, 10 and 15 Gy) and two concentrations with two different durations of two chemical mutagens, NMU 0.01% 20h and 0.02% 8h) and EMS (0.1% 20h and 0.2% 8h). Results indicated that chemical mutagens, particularly NMU are not only more effective but also efficient than physical mutagens in inducing mutations in chickpea. Mutagenic effectiveness and efficiency showed differential behaviour depending upon mutagen and varietal type. Chemical mutagens were more efficient than physical in inducing cholorophyll as well as viable and total number of mutations. Among the mutagens NMU was the most potent, while in the physical, gamma rays were more effective. Out of four mutagens, NMU was the most effective and efficient in inducing a high frequency and wide spectrum of chlorophyll mutations in the M2 followed by fast neutrons. While gamma rays showed least effectiveness, EMS was least efficient mutagens. Major differences in the mutagenic response of the four cultivars were observed. The varieties of desi type were more resistant towards mutagenic treatment than kabuli and green seeded type

  4. Relative mutagenic effects of ionizing radiations and alkylating chemicals in rice

    International Nuclear Information System (INIS)

    Studies were undertaken on the relative mutagenic effects of three ionizing radiations (X-rays, gamma rays, fast neutrons) and five alkylating chemicals (DES, EMS, MMS, NMH, MNNG) in rice. Observations on seed germination, seedling survival, seedling height, seed fertility and chlorophyll deficient chimeras were made in the M1 generation. The M2 generation was grown as M1 ear progenies. Chlorophyll mutation frequency, segregation percentage and mutant spectrum were estimated. The mutagenic effectiveness and efficiency were also worked out. Radiations were more effective than chemical mutagens. Mutagenic effectiveness decreased with increase in doses of mutagens. This was due to the failure of mutation frequency to increase proportionately with increase in mutagen doses. Mutagenic efficiency was higher for radiations when estimated on the basis of lethality and injury whereas, based on sterility, chemical mutagens were more efficient. Mutagenic efficiency also decreased with increase in doses of mutagens. The most effective as well as efficient radiation was fast neutrons. Among chemical mutagens the most effective was NMH whereas the most efficient was EMS. (author)

  5. Induced genetic variability in chickpea (Cicer arietinum L.) II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens

    International Nuclear Information System (INIS)

    Comparative mutagenic effectiveness and efficiency of gamma rays and Ethyl methane sulphonate (EMS) were studied in two desi (Pb2000 and C44), one kabuli (Pb1) and one desi x kabuli introgression line (CH40/91) of chickpea. The treatments included two doses each of gamma rays and EMS calculated on the basis of their LD30. The results revealed that EMS was almost seven times more effective and its efficiency was two times higher than that of gamma rays. Mutagenic effectiveness and efficiency were found to depend upon mutagen type and the genotype and both were higher at lower doses of EMS in three genotypes except in desi genotype C44. The overall trend of mutagenic effectiveness and efficiency in both gamma radiation and EMS was in the order i.e. CH40/91 greater than Pb2000 greater than Pb1 greater than C44. The introgression line desi x kabuli genotype was found to be most resistant towards mutagenic treatments than desi and kabuli types

  6. Molecular dosimetry of the chemical mutagen ethyl methanesulfonate

    International Nuclear Information System (INIS)

    Extending previous work with E. coli and mammalian cells in culture, forward-mutation frequencies induced by ethyl methanesulfonate (EMS) were quantitatively compared in Neurospora crassa and Saccharomyces cerevisiae under standardized conditions. Concomitantly, the actual dose to DNA was measured by determining the amount of radioactivity bound to DNA after treatment with tritium-labeled EMS. After exposure to EMS (2.5-50 mM), alkylation levels in N. crassa and S. cerevisiae were similar to those previously determined in E. coli and cultured mammalian cells. Consistently, there was a slightly less than proportional increase of the DNA alkylation level with the exposure concentration of the mutagen. Forward mutagenesis induced in yeast and N. crassa showed exponential kinetics with exponents of 1.5 and 2.6, respectively. These results are similar to those previously reported with E. coli, which differed from the results with cultured mammalian cells, where a linear dose-effect relationship between exposure and genetic effect was observed. These differences may reflect differences in the fate of EMS-induced adducts by cellular DNA repair systems, but are not due to initial differences in DNA alkylation levels. The fate and persistence of specific DNA adducts potentially responsible for pre-mutagenic changes are under investigation. (orig.)

  7. Radiation-equivalent dose of chemical mutagens: problems and perspectives

    International Nuclear Information System (INIS)

    The radiation equivalent unit was intended as a means of comparing chemically-induced genetic effects with one another and with that of radiation. It was to be a unit of convenience with no absolute value. In this concept the fact that radiations and chemicals might act by different mechanisms is unimportant since only the effect is compared. Although dissimilarity of mechanism is of no consequence when comparing effects of different mutagens with the same end-point in the same species, it is important that the mechanism be similar when extrapolating to man from the effect of a given mutagen in another system. That is why mammalian systems are preferable to sub-mammalian systems for this purpose

  8. The effective use of physical and chemical mutagen in the induction of mutation for crop improvement in Malaysia

    Energy Technology Data Exchange (ETDEWEB)

    Abdul Rahim Harun [Malaysian Institute for Nuclear Technology Research, Bangi, Selangor (Malaysia)

    2001-03-01

    The earliest work of induced mutations breeding program in Malaysia was reported in 1967. The project was carried out by Rubber Research Institute of Malaysia using x-radiation in an attempt to improve rubber trees for dwarfism and disease resistance. Subsequently, more efforts were taken up by the universities to promote the technology for genetic changes and creation of new genetic resources, particularly in crops that are not easily achievable through conventional techniques. Gamma radiation is always been used as physical mutagen, while ethyl methane sulfonate (EMS) was a popular chemical mutagen used in induced mutation breeding in the country. Gamma rays is an effective mutagen to which more than 30 potential mutants were produced up to now through mutagenesis of several important food crops and ornamental plants. Although chemical mutagen such as EMS were reported being used, the result is not so convincing as compared to gamma radiation. Malaysian Institute for Nuclear Technology Research (MINT) has initiated and promoted nuclear technique in mutation breeding for the improvement of importance food crops such as rice, legume and other potential crops for export, like fruit trees and ornamentals. Gamma radiation is the main source of mutagen used in mutation-breeding programme at MINT. The effectiveness of these two mutagens were verified with mutants derived through induced mutation breeding in the country which some mutant has shown outstanding improvement and released as new varieties and cultivars. This paper summarises and discuss the effects as well as achievement attained through the use of ionizing radiation and chemical mutagen in plant mutation breeding in Malaysia. (author)

  9. The effective use of physical and chemical mutagen in the induction of mutation for crop improvement in Malaysia

    International Nuclear Information System (INIS)

    The earliest work of induced mutations breeding program in Malaysia was reported in 1967. The project was carried out by Rubber Research Institute of Malaysia using x-radiation in an attempt to improve rubber trees for dwarfism and disease resistance. Subsequently, more efforts were taken up by the universities to promote the technology for genetic changes and creation of new genetic resources, particularly in crops that are not easily achievable through conventional techniques. Gamma radiation is always been used as physical mutagen, while ethyl methane sulfonate (EMS) was a popular chemical mutagen used in induced mutation breeding in the country. Gamma rays is an effective mutagen to which more than 30 potential mutants were produced up to now through mutagenesis of several important food crops and ornamental plants. Although chemical mutagen such as EMS were reported being used, the result is not so convincing as compared to gamma radiation. Malaysian Institute for Nuclear Technology Research (MINT) has initiated and promoted nuclear technique in mutation breeding for the improvement of importance food crops such as rice, legume and other potential crops for export, like fruit trees and ornamentals. Gamma radiation is the main source of mutagen used in mutation-breeding programme at MINT. The effectiveness of these two mutagens were verified with mutants derived through induced mutation breeding in the country which some mutant has shown outstanding improvement and released as new varieties and cultivars. This paper summarises and discuss the effects as well as achievement attained through the use of ionizing radiation and chemical mutagen in plant mutation breeding in Malaysia. (author)

  10. Analysis of EMS Mutagenized Soybean by Combination of DOP-PCR and GS-FLX

    International Nuclear Information System (INIS)

    Ethylmethanesulfonate (EMS) has been commonly used to induce mutations for various organisms because it led to irreversible mutations with a high level of frequency. With relatively few individuals, saturated mutagenized populations could be generated by chemical mutagens. Since high-throughput sequencing instruments, such as GS-20 or GS-FLX from Roche/454 Life Sciences are now available, characterization of nucleic acids and massive mutant analysis are more feasible. Due to the requirement of sequence information and high cost for designing primers, degenerate oligonucleotide primed PCR (DOP-PCR) instead of direct sequencing was used for single nucleotide polymorphisms (SNPs) survey. In this study, we screen point mutation in soybean mutants generated by EMS mutagenesis using a combination of DOP-PCR methodology and GS-FLX. Four different modified DOP-PCR primers were used for amplifying genomic DNA of three soybean genotypes, Sinpaldalkong 2, SS2-2 and 25-1-1. Then, nucleotide sequences of these amplified PCR products were analyzed by GS-FLX. Different number and length of contigs and singlets were constructed depending on soybean genotypes and nucleotide identity, after sequences were trimmed and aligned. With 100% in identity, average 1,100 contigs and 7,000 singlets were formed in each soybean genotype. In order to survey sequence polymorphisms, POLYBAYES was used with base quality consideration. A total of 1,187 putative SNPs were detected, and these polymorphisms should be reconfirmed by direct sequencing after a homology search against GenBank databases. (author)

  11. Analysis of EMS mutagenized soybean by combination of DOP-PCR and GS-FLX

    International Nuclear Information System (INIS)

    Ethylmethanesulfonate (EMS) causing point mutations were commonly used to induce mutations for various organisms because this leaded to irreversible mutations with high level of frequency. With relatively few individuals, saturated mutagenized populations could be generated by chemical mutagens. Since high-throughput sequencing instruments, such as GS-20 or GS-FLX from Roche/454 Life Sciences, are now available, characterization of nucleic acids and massive mutant analysis are more feasible. Due to the requirement of sequence information and high cost for designing primers, degenerate oligonucleotide primed PCR (DOP-PCR) instead of direct sequencing was used for single nucleotide polymorphisms (SNPs) survey. In this study, we screen point mutation in soybean mutants generated by EMS mutagenesis using combination of DOP-PCR methodology and GS-FLX. Four different modified DOP-PCR primers were used for amplifying genomic DNA of three soybean genotypes, Sinpaldalkong 2, SS2-2 and 25-1-1. And then, nucleotide sequences of these amplified PCR products were analyzed by GS-FLX. Different number and length of contigs and singlets were constructed depending on soybean genotypes and nucleotide identity, after sequences were trimmed and aligned. With 100% in identity, average 1,100 contigs and 7,000 singlets were formed in each soybean genotype. In order to survey sequence polymorphisms, POLYBAYES was used with base quality consideration. A total of putative 1,187 SNPs were detected and these polymorphisms should be reconfirmed by direct sequencing after homology search against GenBank databases. (author)

  12. Modifying the effects of fast neutrons in rice seeds by post-treatment with chemical mutagens

    International Nuclear Information System (INIS)

    Dormant rice seeds were exposed to 290-1160 rad of neutrons, dehulled and then soaked in buffered aqueous solutions of either 0.03M EMS or 0.005M MMS for 15h at 30 deg. C. The neutron plus EMS treatment did not affect seedling height and seedset as much as did the neutron plus MMS treatment, which caused marked reductions over those due to neutrons alone. The mutation frequency (Msp) in all neutron doses was slightly to markedly increased by EMS, and reduced by MMS. Neutrons + EMS was found to be an efficient mutagenic combination whereas neutrons + MMS was inefficient (Msp/I). Differences in the synergistic effects of these combined treatments may be due to the specific biological action of the chemicals as determined by their physical properties. An idea that the chemicals may have acted essentially as selective factors for survival of neutron-damage or mutated tiller initials is briefly discussed. (author). 26 refs, 3 tabs

  13. Enhanced sensitivity to mutagens - EMS, MH, SM by pre-soaking - A taxometric study based on M1 parameters in finger millet

    International Nuclear Information System (INIS)

    Pre-soaking seeds before treatment enhances sensitivity to many chemical mutagens; but little work has been done with maleic hydrazide (MH), a chromosome breaking agent and preferential inducer of micromutation and streptomycin (SM), a cytoplasmic mutagen. In the present investigation on pre-soaking (PS) effects in finger millet (Eleusine coracana, Gaertn), we included these two mutagens, besides one commonly used mutagen, EMS to determine a common effective PS range usingM1seedling traits. Since differentM1parameters, mutagens and their doses showed different peaks of response, we adopted a taxometric approach using all characters together. Combinations of chemicals, doses and sixM1seedling attributes gave 48 characters for the numerical classification of PS periods (0, 8, 10, 12, 14, 16 and 18 h) as OTUs. Dendrogram from the similarity matrix using UPGMA clustering showed two clusters : (1) Cl. 1 of three OTUs (0, 8 and 10 h PS) and (2) Cl.2 of four OTUs(12-18h PS).We considered 12-18h as effective PS range and 0-10h as ineffective for all kinds of mutagens. The effective range would contain the major peak of sensitivity; the ineffective range might show a small peak. We confirmed these inferences with SM induced albinism as an indicator of plastid mutations. Higher doses shifted the peak within the effective range towards lower PS and low dose towards longer PS. Taxometrics could be usefully adopted in mutagenesis studies. (author)

  14. Mutagens and carcinogens - Occurrence and role during chemical and biological evolution

    Science.gov (United States)

    Giner-Sorolla, A.; Oro, J.

    1981-01-01

    The roles of mutagenic and carcinogenic substances in early biologic evolution is examined, along with terrestrial and extraterrestrial sources of mutagens and carcinogens. UV solar radiation is noted to have served to stimulate prebiotic life while also causing harmful effects in plants and animals. Aromatic compounds have been found in meteorites, and comprise leukemogens, polycyclic hydrocarbons, and nitrasamine precursors. Other mutagenic sources are volcanoes, and the beginning of evolution with mutagenic substances is complicated by the appearance of malignancies due to the presence of carcinogens. The atmosphere of the Precambrian period contained both mutagens and early carcinogens and, combined with volcanic activity discharges, formed an atmospheric chemical background analogous to the background ionizing radiation. Carcinogenesis is concluded to be intrinsic to nature, having initiated evolution and, eventually, cancer cells.

  15. Modification of tolerance of oats to crown rust induced by chemical mutagens

    International Nuclear Information System (INIS)

    Seeds of crown rust (Puccinia coronata) susceptible cultivated oats (Avena sativa) were treated with the mutagenic chemical ethyl methanesulphonate (EMS), and pure lines derived from these treated seeds were tested in later generations for the relative amount of reduction in yield and seed weight caused by crown rust infection. In the absence of crown rust, the yield of most of the treated lines was greatly reduced. The overall means of the treated lines for both yield and seed weight response to infection were significantly lower than the control, but 10 lines significantly exceeded the control for yield response and 15 exceeded it for seed weight response. Recurrent EMS treatment of once-treated lines rated as tolerant resulted in groups of lines that were more tolerant, on the average, than groups of lines from recurrently treated lines rated as susceptible. A few of the recurrently treated individual lines derived from tolerant parents had a higher degree of tolerance than their parental lines. EMS treatment of diploid (A. strigosa) and tetraploid (A. abyssinica) oats resulted in groups of lines showing significant genetic variance for response to crown rust, indicating that treatment had induced real genetic change. A few diploid lines were a little more tolerant than their control, but none of the tetraploid lines showed any consistent improvement. (author)

  16. Effect of physical and chemical mutagens on seed germination and survival of seedling in Lycopersicon esculentum Mill

    International Nuclear Information System (INIS)

    Dry and healthy seeds of Lycopersicon esculentum Mill. var. Co-2 were irradiated with gamma rays at 10 KR, 20 KR, 30 KR, 40 KR and 50 KR. The percentage of seed germination was directly proportional to the dose given. The survival percentage decreased with higher doses. Concentration of EMS and NMU applied, ranged from 10 mM to 50 mM and 1 mM to 5 mM, respectively. The duration of soaking of seed was 4 hours in distilled water and 4 hours in mutagenic agents. In treated seeds, the percentage of germination and survival of seedlings decreased with an increase in concentration of these chemical mutagens. These observations are discussed in detail. (author). 11 refs

  17. Combined effects of a chemical mutagen and radiation sterilized diet in mutagenicity and reproduction studies in the same mouse

    International Nuclear Information System (INIS)

    The possible intensification of the mutagenic effect of cyclophosphamide (Endoxan) by the feeding of a radiation-sterilized diet (dose, 4.5 Mrad) was studied in 2000 NMRI/Han mice. In a dominant lethal test, males were pretreated with 100 mg Endoxan/kg body weight. The greatest sensitivity towards Endoxan was observed during the late-spermatid stage. No significant differences were detected between the control group (Endoxan plus non-irradiated diet) and the experimental group (Endoxan plus radiation-sterilized diet). In this test, radiation-sterilized feed showed no co-mutagenic effect when combined with Endoxan treatment. In a reproduction study of 7 months duration (continuous mating without lactation periods), the females were treated every 2 wk with 20 mg Endoxan/kg body weight. The decline in litter size with increasing number of litters (i.e. with advancing age of the females) was more pronounced after treatment with the chemical mutagen than in the untreated group. Increases in the frequency of abortions and in premature sterility resulted from Endoxan treatment. During the entire observation period, no effects from the intake of radiation-sterilized food were detected. (author)

  18. [Revision of th distribution of chromosome aberrations induced by chemical mutagens using the BUDR label].

    Science.gov (United States)

    Chebotarev, A N; Chernyshova, N A

    1990-08-01

    Cell distribution was analysed with the help of the BrDU label for the number of chromosome aberrations and breaks induced by one-center (thiophosphamide and phosphamide) and two-center (dipine and fotrine) mutagens at the stage G0 in the Ist mitosis of human lymphocytes harvested at different times of culturing (from 56 to 96 h). The comparison was made between the type of aberration distribution in cells and the dependence of their frequency on the harvesting point for various mutagens. Poisson aberration distribution in cells for two-center mutagens was found to correspond to their constant frequency observed at different times of harvesting. On the other hand, for one-center mutagens, a geometrical distribution of chromosome breaks corresponded to an exponential decrease in their frequency in time. It is suggested that two-center chemical mutagens and ionizing radiation cause largely short-live damages which are realized into chromosome aberrations rather quickly (during one cell cycle). One-center mutagens, however, cause such damages that the probability of their transformation into chromosome aberrations is decreasing rather slowly in time, under the exponential law, and their realization into chromosome aberrations can occur in subsequent cell cycle. PMID:2258036

  19. Mutagenic effect of ionizing radiation and chemical and environmental agents in Tradescantia

    International Nuclear Information System (INIS)

    The studies covered the following problems: an influence of some environmental agents on the mutagenic effectiveness of ionizing radiation, interaction between ionizing radiation and chemical mutagens in the induction of somatic mutations and also an application of Tradescantia model system for biological monitoring. The studies showed that the pretreatment of Tradescantia plants with sodium fluoride or the modification of the soil composition with dolomite admixture, visibly influences plants radiosensitivity. The analysis of the changes in the dose-response curves suggested that the employed agents were influencing in different ways the repair processes of the DNA. The studies on the interaction between agents proved that the synergistic effect occurs in case of combined action of ionizing radiation with such chemical mutagens as ethyl methansulfonate or 1,2 dibromomethane. It was also discovered that in the range of low doses the effect was proportional to radiation dose and total exposition to chemical mutagen. The field application of Tradescantia method defined the mutagenicity of air pollution in the Cracow area. The highest frequencies of mutations were detected after the Chernobyl accident and after the damage of the filters in the Pharmaceutical Plant. The applied method was evaluated in respect of its usefulness for biological monitoring of environmental pollution. 163 refs. (author)

  20. Suppressive effects of coffee on the SOS responses induced by UV and chemical mutagens

    International Nuclear Information System (INIS)

    SOS-inducing activity of UV or chemical mutagens was strongly suppressed by instant coffee in Salmonella typhimurium TA1535/pSK1002. As decaffeinated instant coffee showed a similarly strong suppressive effect, it would seem that caffeine, a known inhibitor of SOS responses, is not responsible for the effect observed. The suppression was also shown by freshly brewed coffee extracts. However, the suppression was absent in green coffee-bean extracts. These results suggest that coffee contains some substance(s) which, apart from caffeine, suppresses SOS-inducing activity of UV or chemical mutagens and that the suppressive substance(s) are produced by roasting coffee beans. (Auth.)

  1. EMS induced mutagenicity in pollen mother cells of Sesbania Pea (Sesbania cannabina Poir.

    Directory of Open Access Journals (Sweden)

    Girjesh Kumar and Nitisha Srivastava

    2011-06-01

    Full Text Available Mutagenic effect of EMS on pollen mother cells of Sesbania cannabina has been investigated in the present cytogeneticalobservation. The progenies were obtained from seeds treated with four different time durations (1, 3, 5, and 7 hours of 0.5%solution of ethyl methane sulphonate (EMS. Study revealed various types of chromosomal aberrations at different doses ofEMS. Increase in chromosomal aberrations was dose dependent manner. Pollen mother cells of Sesbania cannabina exhibited anincreased incidence of chromosomal bridges, unorientation, laggards and precocious movement etc. at each dose of treatment.Anaphasic and telophasic bridges were major abnormalities and found to be highest at 3 hr dose of treatment. Anaphasic bridgeswere more prominent than telophasic bridges and percentages of different types of bridges at anaphase and telophase were alsostudied and scored.

  2. Discerning the Chemical Composition and Mutagenic Effects of Soy Biodiesel PM

    Science.gov (United States)

    Discerning the Chemical Composition and Mutagenic Effects of Soy Biodiesel PM David G. Nashab, Esra Mutluc, William T. Prestond, Michael D. Haysb, Sarah H. Warrenc, Charly Kingc, William P. Linakb, M. lan Gilmourc, and David M. DeMarinic aOak Ridge Institute for Science and Ed...

  3. Effect of gamma ray and some chemical mutagens sorghum for forage sorghum vulgare

    International Nuclear Information System (INIS)

    This investigation was carried out to study the effect of gamma ray doses (100, 200,400,600 and 800 Gry,DES and SA by concentration (0.25%, 0.75%, 0.10% and 1.5%), on yield components and chemical characteristics (Protein, ash, fiber and charbohydrate%) in the two varieties (Piper and Green Leaf), of Sorghum for forage during M1, M2 and M3 generations . The experiments was carried out in field belonging to the Atomic energy Authority, Inshas during 1990. 1991 and 1992 Seasons. The results of the study revealed that piper variety was better than Green leaf variety in most of the studied characters. Treatment by gamma ray was more effective in increasing means of the studied characters than the two chemical mutagens(DES and SA). The low doses of studied mutagens were more effective than the high doses in increasing the studied traits. Irradiation by Gamma-ray increased the variability in the studied traits than the both chemical mutagens. SO, it could be concluded that, it is better to use Gamma ray than the chemical mutagens in breeding program for sorghum. 18 tabs., 58 refs

  4. Dietary Exposure of Nigerians to Mutagens and Estrogen-Like Chemicals

    Directory of Open Access Journals (Sweden)

    Iyekhoetin Matthew Omoruyi

    2014-08-01

    Full Text Available Food and drinking water are poorly delineated sources of human exposure to chemical food mutagens and endocrine-disrupting chemicals. In this study, we investigated the presence of mutagens and chemicals exhibiting estrogenic activity in the daily diet of Nigerians, using in vitro assays. Commercially processed foods or snacks and various brands of pure water sachets were extracted by solid-phase extraction and liquid-liquid extraction, respectively. Mutagenicity was determined by the conventional Ames test and two complementary assays on two strains of Salmonella (TA 100 and TA 98, while the estrogenic activity was assessed by a yeast bioluminescent assay, using two recombinant yeast strains (Saccharomyces cerevisiae BMAEREluc/ERα and S. cerevisiae BMA64/luc. A third of the food varieties investigated (chin-chin, hamburger, suya and bean cake were mutagenic in all three assays, either in the presence or absence of S9 mix. Of the packed water samples, five out of the sixteen investigated (31%, were found to be estrogenic, with estradiol and bisphenol A equivalents ranging from 0.79 to 44.0 ng/L and 124.2 to 1,000.8 ng/L, respectively. Hence, although the current situation in Nigeria does not appear to be substantially worse than, e.g., in Europe, regular monitoring is warranted in the future.

  5. Experiments with mutagen treatments of rice

    International Nuclear Information System (INIS)

    Experiments were carried out with rice and barley to study the effects of ionizing radiations and chemical mutagens on the basis of seedling growth reduction. The effects of the chemical mutagens ethyl methane sulphonate (EMS) and diethyl sulphate (dES) were studied. A new method for the application of chemical mutagens in rice was developed. The radiosenstivity of dormant rice and barley seeds to gamma radiation and fast neutrons was compared. The dose required to produce 50% reduction in rice was 10 times higher with gamma radiations than with fast neutrons. This ratio for barely was 37:1. (author). 4 refs, 6 tabs

  6. The use of physical/chemical mutagens for crop improvements in Indonesia

    International Nuclear Information System (INIS)

    Most research on the use of physical and chemical mutagens for crop improvement in Indonesia are carried out at the Center for Research and Development of Isotopes and Radiation Technology, National Nuclear Energy Agency (Bataan). At the plant breeding section of this center, much progress has been achieved in term of facilities set-up (gamma irradiators, laboratory, greenhouse and experimental fields), manpower and expertise development. Mutation breeding activities were initially started in rice in 1972, in attempts to improve the protein content of rice grain. During this earlier time, researches on detecting more effective mutagen treatments, using physical and chemical mutagenesis, were conducted in different plant species. The use of chemical mutagens have recently been very limited and it tends to be abandoned simply because of its unpractical treatment procedures working with it and less effective results. Nowadays, most induced mutations in plant breeding are primarily dependent on the use of physical mutagen i.e. gamma irradiation which is emitted from Cobalt-60 source. The effective use of gamma irradiation in plant breeding has been proven by results of finding useful mutant varieties for the country level. Major achievements were the release of some mutant varieties by the Department of Agriculture of Indonesia. These mutant varieties included 6 varieties for rice, 3 for soybean, and 1 for mungbean. Meanwhile, some promising mutant lines of other important crops such as peanuts, sorghum, banana, onions etc. are now being investigated in the field experiments. The effective use of gamma irradiation seems to vary between crop species or varieties being investigated. Experiences on breeding food crops, restricted on self-pollinated crops, the effective dose treatments of gamma irradiation on the seed materials were found to vary between 10-30 Gy. Some experiment results on the use of physical and chemical mutagens for crop improvements are discussed here

  7. The use of physical/chemical mutagens for crop improvements in Indonesia

    Energy Technology Data Exchange (ETDEWEB)

    Soeranto, H.; Manurung, Simon; Masrizal [National Nuclear Energy Agency of Indonesia, Jakarta (ID)] [and others

    2001-03-01

    Most research on the use of physical and chemical mutagens for crop improvement in Indonesia are carried out at the Center for Research and Development of Isotopes and Radiation Technology, National Nuclear Energy Agency (Bataan). At the plant breeding section of this center, much progress has been achieved in term of facilities set-up (gamma irradiators, laboratory, greenhouse and experimental fields), manpower and expertise development. Mutation breeding activities were initially started in rice in 1972, in attempts to improve the protein content of rice grain. During this earlier time, researches on detecting more effective mutagen treatments, using physical and chemical mutagenesis, were conducted in different plant species. The use of chemical mutagens have recently been very limited and it tends to be abandoned simply because of its unpractical treatment procedures working with it and less effective results. Nowadays, most induced mutations in plant breeding are primarily dependent on the use of physical mutagen i.e. gamma irradiation which is emitted from Cobalt-60 source. The effective use of gamma irradiation in plant breeding has been proven by results of finding useful mutant varieties for the country level. Major achievements were the release of some mutant varieties by the Department of Agriculture of Indonesia. These mutant varieties included 6 varieties for rice, 3 for soybean, and 1 for mungbean. Meanwhile, some promising mutant lines of other important crops such as peanuts, sorghum, banana, onions etc. are now being investigated in the field experiments. The effective use of gamma irradiation seems to vary between crop species or varieties being investigated. Experiences on breeding food crops, restricted on self-pollinated crops, the effective dose treatments of gamma irradiation on the seed materials were found to vary between 10-30 Gy. Some experiment results on the use of physical and chemical mutagens for crop improvements are discussed here

  8. Integrated biological chemical approach for the identification of polyaromatic mutagens in surface waters

    OpenAIRE

    GALLAMPOIS Christine

    2012-01-01

    Surface waters are essential for human life, to supply of drinking water and as an important resource for agricultural, industrial and recreational activities. However, tonnes of pollutants enter these surface waters every year. Amongst the substances discharged into the environment, a large number are known to be mutagenic. Effect-directed analysis (EDA) is a tool to identify chemicals responsible for the observed toxic effects. It is based on a combination of chemical and biological analysi...

  9. Study of anti mutagenic and mutagenic effect of different chemicals on clinically isolated strains of pseudomonas aeruginosa

    International Nuclear Information System (INIS)

    This project was undertaken to study the effect of twelve different compounds to test their anti mutagenic and mutagenic activity against clinically isolated strains of Pseudomonas aeruginosa. The effect of these compounds was estimated by counting the number of rifampicin resistant colonies growing in a particular time in a compound. The results were interpreted by plotting graphs between 10g N/NO (Rif R Colonies/ ml) and time to estimate the forward mutation rat. The results revealed that acridine, Basic fuchsin, Caffeine, cycloheximide, Ethidium bromide and Histidine probably have an anti mutagenic effect, while Cysteine, folic acid, Ethyl methane, suplphonate, Manganous Chloride and N-nitrosodietylamine acted as mutagen. Ecoli was used as control through out the study. (author)

  10. Dimethylglycine and chemically related amines tested for mutagenicity under potential nitrosation conditions.

    Science.gov (United States)

    Hoorn, A J

    1989-04-01

    Dimethylglycine (DMG) and the chemically related amino acids glycine, sarcosine (monomethylglycine) and betaine (trimethylglycine) were tested in Salmonella typhimurium strain TA100 after treatment with sodium nitrite under acidic conditions using a modified Ames Salmonella/microsome assay as reported by Colman et al. (1980). The increase in the number of revertants observed both with and without metabolic activation was also induced in the control mixtures without adding the amines. From the subsequent testing of the individual components of the mixtures, we concluded that non-consumed nitrite was responsible for the mutagenic responses observed in the different reaction mixtures, and not the amines themselves. There were no consistent indications of mutagenic activity of the DMG test mixture as compared to the control mixture which exhibited both consistent mutagenic activity and a toxic effect which was not increased by the addition of DMG. In fact, DMG seemed to decrease the toxicity of the control reaction solution to the Salmonella which was clearly observed at the higher doses. DMG cannot be considered mutagenic under the test conditions employed. The same can be said of the other amino acids as well. PMID:2468082

  11. Comparison of the mutagenic potential of 17 physical and chemical agents analyzed by the flow cytometry mutation assay

    Energy Technology Data Exchange (ETDEWEB)

    French, C. Tenley [Cytomation GTX Inc., Fort Collins, CO (United States); Ross, Carley D. [Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO (United States); Keysar, Stephen B. [Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO (United States); Joshi, Dhanashree D. [Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO (United States); Lim, Chang-Uk [Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO (United States); Fox, Michael H. [Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO (United States) and Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523-1618 (United States)]. E-mail: mfox@colostate.edu

    2006-12-01

    Several methods to assess genotoxicity of physical and chemical agents have been developed, most of which depend on growing colonies in selective medium. We recently published a new method for detecting mutations in the CD59 gene in a Chinese hamster ovary cell line that contains a single copy of human chromosome 11 (CHO A{sub L}). The assay is based on detecting the surface expression of CD59 with monoclonal antibodies using flow cytometry. The capabilities of this flow cytometry mutation assay (FCMA) to detect mutations from a wide variety of genotoxic agents are described here. There was a 400-fold separation between CD59{sup -} and CD59{sup +} populations based on fluorescence intensity. Small numbers of negative cells mixed in with positive cells were detected in a highly linear fashion. Mutation dose response curves over a dose range yielding 80% to 20% survival are shown for ethyl methane sulfonate (EMS), mitomycin C (MMC) and lead acetate. EMS and lead acetate exhibited a threshold in response while MMC had a linear dose response over the full dose range. The mutant fraction was measured over time periods ranging up to 35 days following treatment. The mutant fraction peaked at different times ranging from 6 to 12 days after treatment. An additional 14 chemical and physical agents including point mutagens, heavy metals, ionizing and UV radiation, and DNA intercalators and cross linkers, were analyzed for mutagenic potential after doses giving 80% to 20% survival. The results presented here demonstrate the sensitivity and broad-ranging capability of the FCMA to detect mutations induced by a variety of genotoxic agents.

  12. Use of physical/chemical mutagens in plant breeding program in Vietnam

    Energy Technology Data Exchange (ETDEWEB)

    Tran Duy Quy; Nguyen Huu Dong; Bui Huy Thuy; Le Van Nha; Nguyen Van Bich [Agricultural Genetics Institute, Hanoi (Viet Nam)

    2001-03-01

    Among more than 1870 new plant varieties formed by mutation breeding in the world, 44 varieties of different plants were formed by Vietnamese scientists. Research on induced mutation in Vietnam started in 1966, was promoted in Agricultural Institute, Cuu Long Delta Rice Research Institute, Institute of Food Crop Research, and Agriculture Universities, and has produced varieties of rice, maize, soybean, peanut, tomato, jujuba, green bean etc using physical and chemical mutagens: Irradiation with gamma rays or neutrons, and use of such chemicals as dimethylsulfate (DMS), diethylsulfate (DES), ethyleneimine (EI), N-nitrosomethylurea (NUM), N-nitrosoethylurea (NEU), and sodium azide (NaN{sub 3}). In the present report, the results of cytological and genetic effects in M1 plants, the frequency and spectrum of chlorophyll and morphological mutants, the mutants obtained and the genetic nature of the next generation are described, particularly for the case of rice. Radiation dose and dose rate used as mutagens are also reported. (S. Ohno)

  13. Use of physical/chemical mutagens in plant breeding program in Vietnam

    International Nuclear Information System (INIS)

    Among more than 1870 new plant varieties formed by mutation breeding in the world, 44 varieties of different plants were formed by Vietnamese scientists. Research on induced mutation in Vietnam started in 1966, was promoted in Agricultural Institute, Cuu Long Delta Rice Research Institute, Institute of Food Crop Research, and Agriculture Universities, and has produced varieties of rice, maize, soybean, peanut, tomato, jujuba, green bean etc using physical and chemical mutagens: Irradiation with gamma rays or neutrons, and use of such chemicals as dimethylsulfate (DMS), diethylsulfate (DES), ethyleneimine (EI), N-nitrosomethylurea (NUM), N-nitrosoethylurea (NEU), and sodium azide (NaN3). In the present report, the results of cytological and genetic effects in M1 plants, the frequency and spectrum of chlorophyll and morphological mutants, the mutants obtained and the genetic nature of the next generation are described, particularly for the case of rice. Radiation dose and dose rate used as mutagens are also reported. (S. Ohno)

  14. Effects of gamma rays and chemical mutagens on quantitative characteristics of hemp

    Energy Technology Data Exchange (ETDEWEB)

    Zhatov, A.I.

    1979-01-01

    Observations of hemp plants produced by seeds irradiated with gamma rays (1 or 15 kR) or treated with chemical mutagens (soaking in 0.01% ethylenimine for 18 h or in 0.05% ethylmethanesulfonate for 12 h) showed the appearance of both positive and negative hereditary characteristics. Combination of the wider spectrum of mutant plants with those from appropriate breeding practices could lead to the development of new varieties of hemp with superior commercial characteristics.

  15. Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS mutagenized plants

    Directory of Open Access Journals (Sweden)

    Silué, S.

    2013-01-01

    Full Text Available The aim of this study was to describe the embryos abortion process and the inheritance of the embryos abortion trait in Phaseolus vulgaris plants deficient in seed development. These plants were isolated within the second generation of an ethyl methanesulfonate (EMS TILLING population of P. vulgaris cv. 'BAT93'. Mutant embryos show abnormalities mainly in suspensors, shoot apical meristem (SAM and cotyledons from the globular to the cotyledon stages and abort before maturity compared to those observed in wild-type samples. Mutant embryos show also hyperhydricity and contain low amount of chlorophyll. Genetic analyses of F1, F2 and F3 populations from the crosses carried out between the mutagenized plants with aborting embryos and the wild-type plants indicated that the embryo abortion phenotype is maternally inherited and controlled by a single recessive gene. These Phaseolus mutant plants with aborting embryos constitute a valuable material for plant embryogenesis studies.

  16. Mutagenic effectiveness and efficiency of gamma rays, EMS and their combination in Indian mustard

    International Nuclear Information System (INIS)

    Rapeseed-mustard, the second most important oilseed crop after groundnut has shown considerable progress in terms of productivity. However, the potential yield still could not be realised due to susceptibility of released varieties to Alternaria blight. Mutation breeding is an important means for developing resistance as well as for extending the range of plant architecture. Four varieties of Indian mustard viz.. RH 30, RH 819, Varuna and Kranti were exposed to four doses of gamma rays, four doses of EMS and four doses of both gamma rays and EMS. Pollen sterility was estimated in the M1 generation and was used to calculate the mutagenic efficiency. The chlorophyll mutation frequency in M2 was highest in variety RH 819 and among the treatments it was maximum in 80 kR of gamma ray. Total mutation frequency was highest in the combination treatments. Over the varieties 60 kR of gamma rays was most effective and EMS was more efficient than gamma rays and combination treatment. RH 819 and Varuna appeared to be suitable base for improvement in quantitative traits where as Kranti and RH 30 are better bases for induction of Alternaria blight resistance through mutations. (author)

  17. Chemical carcinogenic and mutagenic agents in the workplace, Poland, 2008–2010

    Directory of Open Access Journals (Sweden)

    Katarzyna Konieczko

    2013-04-01

    Full Text Available Background: The aim of this paper is to present a concise but comprehensive information on the occurrence of carcinogenic or mutagenic agents in Polish enterprises and the number of workers exposed to those agents reported to the central register by employers. Objectives and responsibilities of the register, as well as the range and methods of data gathering are discussed. Material and Methods: Data concerning carcinogenic or mutagenic chemical substances and technological processes reported to central register in 2008-2010 were analyzed. Results: In 2008-2010 more than 300 carcinogenic or mutagenic chemical substances were reported to the register. Approximately 2500 plants reported above 150 000 per-person-exposures annually. Among all technological processes regarded as occupational carcinogens, hardwood dusts exposure (about 660 companies; 11 000-13 000 exposed workers each year and exposure to polycyclic aromatic hydrocarbons (PAHs present in coal products (117-125 plantsl 3000 exposed per year were reported. Conclusions: The most widespread carcinogenic/mutagenic substances were: benzene, chromium(VI compounds: potassium dichromate and chromate, chromium(VI trioxide and other chromium compounds, ethylene oxide, asbestos, benzo[a]pyrene and gasoline. The highest number of men was exposed to particular PAHs and benzene , and the majority of women was exposed to benzene, potassium dichromate and chromate, acrylamide, ethylene oxide and gasoline. The lack of clear-cut definitione of occupational exposure to carcinogen creates a problem faced by employers in defining the accurate number of exposed workers. Med Pr 2013;64(2:181–192

  18. Induced mutagenesis of plasmid and chromosomal genes inserted into the plasmid DNA. II. Mutagenic action of chemical factors

    International Nuclear Information System (INIS)

    Following the study of the mutagenic action of UV and γ-radiation on plasmid DNA in vitro, they investigated the induction of mutations under the influence of chemical mutagens on the same DNA of plasmid RSF2124, determining the synthesis of colicine E1 and resistance to ampicillin. The inactivating action of the mutagen was assessed from the yield of transformants resistant to the antibiotic and the mutagenic effect from the loss by colonies of transformants that were capable of releasing colicine into the external medium. In these experiments they mainly used chemical compounds whose mutagenic effect if well known in other systems (transforming and transfecting DNA, microbial viruses). As a result all mutagens tested for their activity were divided into four groups: first group, those exceeding the level of mutagenesis by more than 100-fold above the spontaneous background (hydroxylamine, O-methylhydroxylamine); second group, those exceeding it by a factor of 10 (UV radiation (λ = 254 nm), W-mutagenesis, ionizing radiation, nitrous acid, mitomycin C); third group, those exceeding it by a factor of <10 (indirect UV mutagenesis, nitrous acid, β-chloroethyldiethylamine hydrochloride, nitrosoguanidine); fourth group, no mutagenic effect (acridine orange, ethyl methane sulfonate, sodium azide, 0-β-diethylaminoethylhydroxylamine)

  19. A Review on Mutagenicity Testing for Hazard Classification of Chemicals at Work: Focusing on in vivo Micronucleus Test for Allyl Chloride.

    Science.gov (United States)

    Rim, Kyung-Taek; Kim, Soo-Jin

    2015-09-01

    Chemical mutagenicity is a major hazard that is important to workers' health. Despite the use of large amounts of allyl chloride, the available mutagenicity data for this chemical remains controversial. To clarify the mutagenicity of allyl chloride and because a micronucleus (MN) test had not yet been conducted, we screened for MN induction by using male ICR mice bone marrow cells. The test results indicated that this chemical is not mutagenic under the test conditions. In this paper, the regulatory test battery and several assay combinations used to determine the genotoxic potential of chemicals in the workplace have been described. Further application of these assays may prove useful in future development strategies of hazard evaluations of industrial chemicals. This study also should help to improve the testing of this chemical by commonly used mutagenicity testing methods and investigations on the underlying mechanisms and could be applicable for workers' health. PMID:26929826

  20. Application of chemical mutagens and radiation in breeding buckwheat for larger seeds

    International Nuclear Information System (INIS)

    Full text: In 1974, seeds of the Viktoriya variety of buckwheat were treated with 20-30 krad gamma radiation and chemical mutagens in the Biophysics Department of the Kishinev Agricultural Institute. For the chemical mutagen treatment, we used N-ethylnitroso-urea NEH (0.025 and 0.012%), N-methylnitroso-urea NMH (0.01 and 0.005%), ethylenimine EI (0.01 and 0.005%), dimethyl sulphate DMS (0.01 and 0.005%) and 1.4-bis-diazoacetyl butane DAB (0.01 and 0.05%). Since some investigators think that different results are produced by changing the order of the treatment, we treated seeds with chemical mutagens before and after irradiation and this was followed by drying. A total of 2400 seeds were treated. Selection started with M2 seeds produced by M1 plants. The thousand seed weight of the best ones ranged from 40.7 to 47.8 g, which was 11.9-18.7 g heavier than the control. The large seed size thus selected was heritable. Since larger seeds are very important for the creation of high yielding varieties buckwheat, only families with these characteristics were selected for further work. We observed even some further increase in seed weight in the next generation. It was observed that when planting large seeds, after six days of growth the cotyledons were significantly larger than in the control plants. This characteristic was used in selecting for a high yielding large-seed variety of buckwheat. The plants were selected twice: once for development of large cotyledon leaves and the second time for plant yield. In the fourth generation, the families thus obtained continued to be studied in greenhouse experiments and the same time be propagated under field conditions. The seeds of these families were then combined and under the name Podolyanka in 1976 were subjected to competitive variety testing. Following the competitive variety testing the mutant variety Podolyanka was released in 1984. It is high yielding (2950 kg/ha), has a short vegetation period (matures 17-18 days

  1. Effective use of physical/chemical mutagens in crop hybrid breeding in China

    Energy Technology Data Exchange (ETDEWEB)

    Liu Luxiang; Wang Jing [Chinese Academy of Agricultural Sciences, Institute for Application of Atomic Energy, Beijing (China)

    2001-03-01

    Crop heterosis utilization was one of the greatest achievements in the agriculture production in the 20th century. It is proved that every breakthrough in crop hybrid breeding was predicated on the discovery or successful development of new heterosis germplasm. In recent years, in order to open up the scope and ways of using crop heterosis, it has been paid much close attention to apply mutation techniques to hybrid breeding. Useful tool materials like male sterile mutant lines, fertile restoration mutants in many crops have been obtained by effective use of physical/chemical mutagens. Brief introduction is made in this paper on the newest research improvement concerning the effective use of the techniques of mutation induction in China to create special useful genes, enrich the diversity of germplasm and promote the rapid development of crop hybrid breeding. (author)

  2. Effective use of physical/chemical mutagens in crop hybrid breeding in China

    International Nuclear Information System (INIS)

    Crop heterosis utilization was one of the greatest achievements in the agriculture production in the 20th century. It is proved that every breakthrough in crop hybrid breeding was predicated on the discovery or successful development of new heterosis germplasm. In recent years, in order to open up the scope and ways of using crop heterosis, it has been paid much close attention to apply mutation techniques to hybrid breeding. Useful tool materials like male sterile mutant lines, fertile restoration mutants in many crops have been obtained by effective use of physical/chemical mutagens. Brief introduction is made in this paper on the newest research improvement concerning the effective use of the techniques of mutation induction in China to create special useful genes, enrich the diversity of germplasm and promote the rapid development of crop hybrid breeding. (author)

  3. A QSAR for the Mutagenic Potencies of Twelve 2-Amino-trimethylimidazopyridine Isomers: Structural, Quantum Chemical,and Hydropathic Factors

    Energy Technology Data Exchange (ETDEWEB)

    Knize, M G; Hatch, F T; Tanga, M J; Lau, E V; Colvin, M E

    2005-04-23

    An isomeric series of heterocyclic amines related to one found in heated muscle meats was investigated for properties that predict their measured mutagenic potency. Eleven of the 12 possible 2-amino-trimethylimidazopyridine (TMIP) isomers were tested for mutagenic potency in the Ames/Salmonella test with bacterial strain TA98, and resulted in a 600-fold range in potency. Structural, quantum chemical and hydropathic data were calculated on the parent molecules and the corresponding nitrenium ions of all of the tested isomers to establish models for predicting the potency of the unknown isomer. The regression model accounting for the largest fraction of the total variance in mutagenic potency contains four predictor variables: dipole moment, a measure of the gap between amine LUMO and HOMO energies, percent hydrophilic surface, and energy of amine LUMO. The most important determinants of high mutagenic potency in these amines are: (1) a small dipole moment, (2) the combination of b-face ring fusion and N3-methyl group, and (3) a lower calculated energy of the {pi} electron system. Based on predicted potency from the average of five models, the isomer not yet synthesized and tested is expected to have a mutagenic potency of 0.84 revertants/{micro}g in test strain TA98.

  4. Mutation induction in rice by radiation combined with chemical protectants and mutagens

    International Nuclear Information System (INIS)

    Seeds of the rice variety 'Dourado Precoce' were treated with different combinations of gamma rays, cysteine and EMS or gamma rays, cysteine and dES. Cysteine showed some protection against the effects of gamma radiation and combined gamma-ray + chemical treatments with regard to germination, seedling height and fertility. There are also indications of changes in the spectra of chlorophyll mutations. (author)

  5. Inducing mutations in the mouse genome with the chemical mutagen ethylnitrosourea

    Directory of Open Access Journals (Sweden)

    S.M.G. Massironi

    2006-09-01

    Full Text Available When compared to other model organisms whose genome is sequenced, the number of mutations identified in the mouse appears extremely reduced and this situation seriously hampers our understanding of mammalian gene function(s. Another important consequence of this shortage is that a majority of human genetic diseases still await an animal model. To improve the situation, two strategies are currently used: the first makes use of embryonic stem cells, in which one can induce knockout mutations almost at will; the second consists of a genome-wide random chemical mutagenesis, followed by screening for mutant phenotypes and subsequent identification of the genetic alteration(s. Several projects are now in progress making use of one or the other of these strategies. Here, we report an original effort where we mutagenized BALB/c males, with the mutagen ethylnitrosourea. Offspring of these males were screened for dominant mutations and a three-generation breeding protocol was set to recover recessive mutations. Eleven mutations were identified (one dominant and ten recessives. Three of these mutations are new alleles (Otop1mlh, Foxn1sepe and probably rodador at loci where mutations have already been reported, while 4 are new and original alleles (carc, eqlb, frqz, and Sacc. This result indicates that the mouse genome, as expected, is far from being saturated with mutations. More mutations would certainly be discovered using more sophisticated phenotyping protocols. Seven of the 11 new mutant alleles induced in our experiment have been localized on the genetic map as a first step towards positional cloning.

  6. Mutagenic effects of gamma rays and EMS on frequency and spectrum of chlorophyll mutations in urdbean (Vigna mungo (L.) Hepper)

    International Nuclear Information System (INIS)

    Chlorophyll mutations act as a significant index in the judgment of induced genetic variations in mutagen treated populations. Different types of chlorophyll mutation have been observed in various crop plants. In the current study, the effect of different concentrations (40 kR, 50 kR and 60 kR) of gamma rays, Ethyl Methane Sulphonate (50 mM, 60 mM and 70 mM) in single and combination dose/concentration on the frequency and spectrum of chlorophyll mutation and the effect of VBN 4 urdbean variety to such irradiation dose was observed. Results showed induction of broad spectrum of chlorophyll mutations which included albina, xantha, chlorina and viridis. Among these chlorina type was predominant in all the mutagenic treatments. The albina type of chlorophyll mutants occurred very rarely and was found only at 60 mM of EMS treatment and at 40 kR + 50 mM, 60 kR + 70 mM of combination treatments. Based on the chlorophyll mutation frequency, gamma rays were most effective followed by EMS and combination of treatments. (author)

  7. Effect of physical and chemical mutagens on morphological parameters in garlic

    International Nuclear Information System (INIS)

    Cloves of garlic (Allium sativum Linn.) were treated with various doses of gamma rays and different concentrations of ethylmethane sulphonate, diethyl sulphate and ethyleneimine. The effect of mutagens was studied in respect of morphological parameters such as sprouting, survival, sprout height, plant height, number and size of leaves, number of cloves and weight of bulb. In case of mutagen treatment the percentage of sprouting and survival as well as sprout height were found to be decreased with an increase in the dose/concentration of the mutagen. The effect of mutagen on leaf size and number was inhibitory. However, the number of cloves and weight of bulb were found to be increased at lower dose concentration of mutagens. (author)

  8. Influence of single and combination treatments of physical and chemical mutagen on chlorophyll mutations in Finger Millet

    International Nuclear Information System (INIS)

    Gamma rays, ethyl methane sulfonate (EMS) and their combined treatments influenced differently in producing chlorophyll mutations. A good number of chlorophyll mutants with varied frequencies were recorded in M2 generation. The frequency of chlorophyll mutants was higher at lower doses of gamma rays, EMS and their combination treatments. The most frequently observed mutant was Albino type. The other chlorophyll mutants isolated were Xantha, Viridis, Striata and Tigrina. The frequency of tigrina and striata was lowest in variety A-404 and Hr-374, respectively. The efficiency and effectiveness was high at the lower doses of mutagens in both the varieties. EMS (0.2%) was more effective than the corresponding lower dose of gamma rays for both the varieties. (author). 10 refs., 4 tabs

  9. Comparative effect of irradiation and metabolization of some chemical pollutants in animals based upon mutagenic/carcinogenic activity

    International Nuclear Information System (INIS)

    Polycyclic aromatic hydrocarbons have long been implicated as mutagens and carcinogens. The compounds selected for this study, 3,4-benzo(a)pyrene (BP) and 3-methylcholanterene (MC), were considered the most representative substances of this chemical group. Of the tested metabolites of the first, only 1,2 and 9-hydroxy-BP and diasteromers diolepoxi (7,8,9,10-cis and trans) proved mutagens. BP and MC in mammalian cells produced DNA lesions in the form of single-strand breaks and inhibition of semiconservative synthesis. They did not inhibit rejoining of DNA single-strand breaks induced by ionizing radiation. BP and MC are both mutagens only after metabolic activation, as shown in host-mediated assay and by in-vitro test. In order to establish an equivalence, the effect of three chemicals were investigated: an alkylating agent, BP and MC, the latter requiring metabolic activation. Under the given experimental conditions, 1 rad appeared as equivalent to 55 ng of IOB-82 (a cytostatic), 115 ng of BP and 178 ng of MC. This concept could prove of great importance for evaluating the risks arising from chemical and physical pollutants in man's environment

  10. Quantitative analysis of the relative mutagenicity of five chemical constituents of tobacco smoke in the mouse lymphoma assay.

    Science.gov (United States)

    Guo, Xiaoqing; Heflich, Robert H; Dial, Stacey L; Richter, Patricia A; Moore, Martha M; Mei, Nan

    2016-05-01

    Quantifying health-related biological effects, like genotoxicity, could provide a way of distinguishing between tobacco products. In order to develop tools for using genotoxicty data to quantitatively evaluate the risk of tobacco products, we tested five carcinogens found in cigarette smoke, 4-aminobiphenyl (4-ABP), benzo[a]pyrene (BaP), cadmium (in the form of CdCl2), 2-amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQ) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), in the mouse lymphoma assay (MLA). The resulting mutagenicity dose responses were analyzed by various quantitative approaches and their strengths and weaknesses for distinguishing responses in the MLA were evaluated. L5178Y/Tk (+/-) 3.7.2C mouse lymphoma cells were treated with four to seven concentrations of each chemical for 4h. Only CdCl2 produced a positive response without metabolic activation (S9); all five chemicals produced dose-dependent increases in cytotoxicity and mutagenicity with S9. The lowest dose exceeding the global evaluation factor, the benchmark dose producing a 10%, 50%, 100% or 200% increase in the background frequency (BMD10, BMD50, BMD100 and BMD200), the no observed genotoxic effect level (NOGEL), the lowest observed genotoxic effect level (LOGEL) and the mutagenic potency expressed as a mutant frequency per micromole of chemical, were calculated for all the positive responses. All the quantitative metrics had similar rank orders for the agents' ability to induce mutation, from the most to least potent as CdCl2(-S9) > BaP(+S9) > CdCl2(+S9) > MeIQ(+S9) > 4-ABP(+S9) > NNK(+S9). However, the metric values for the different chemical responses (i.e. the ratio of the greatest value to the least value) for the different chemicals ranged from 16-fold (BMD10) to 572-fold (mutagenic potency). These results suggest that data from the MLA are capable of discriminating the mutagenicity of various constituents of cigarette smoke, and that quantitative analyses are available

  11. Chemical Composition and in Vitro Antimicrobial and Mutagenic Activities of Seven Lamiaceae Essential Oils

    Directory of Open Access Journals (Sweden)

    Laura De Martino

    2009-10-01

    Full Text Available Deeper knowledge of the potentiality of aromatic plants can provide results of economic importance for food and pharmacological industry. The essential oils of seven Lamiaceae species were analyzed by gas chromatography-mass spectrometry and assayed for their antibacterial, antifungal and mutagenic activities. Monoterpenes in the oils ranged between 82.47% (hyssop oil and 97.48% (thyme oil, being mainly represented by oxygenated compounds. The antibacterial activity was evaluated against six pathogenic and five non-pathogenic bacterial strains. Oregano and thyme oils showed the strongest antibacterial activity against the pathogenic ones. The antifungal activity was evaluated against six fungal strains of agrifood interest: the oils tested exhibited variable degrees of activity. Two Salmonella typhimurium strains were used to assess the possible mutagenic activity. No oil showed mutagenic activity. Data obtained let us hypothesise that the use of essential oils could be a viable and safe way to decrease the utilisation of synthetic food preservatives. Further research is needed to obtain information regarding the practical effectiveness of essential oils to prevent the growth of food borne and spoiling microbes under specific application conditions.

  12. Sister chromatid exchanges in the bone marrow cells of in vivo rats induced by gamma radiation and chemical mutagens

    International Nuclear Information System (INIS)

    Sister chromatid exchanges (SCE) in the bone marrow of in vivo rats induced by gamma radiation doses and by the chemical mutagens, mitomycin C (MMC), cyclophosphamide (CP), and sulphonate-methylmethane (SMM), were studied. The purpose was to evaluate the sensitivity and reproducibility of a simplified SCE in vivo detecting system developed in our laboratory and to compare the results obtained with those reported elsewhere. Simplification consisted in administering the amounts of 5-bromo-2'-deoxyuridine (BrdU) necessary to observe the SCE, after first adsorbing the BrdU in activated carbon and then injecting it interperitoneally, into the rats. The results were a longer time in vivo ADN incorporation without convulsions in the rats, and a reduction in the time course as compared to other methods. We observed a basal rate of 3.6+-0.37 SCE/cell and that: 0.44 Gy of gamma radiation induced 7.7+-0.73 SCE/cell; 1.6 μg/g of MMC induced 8.1+-1.20 SCE/cell; 5 μg/g of CP induced 8.25+-1.5 SCE/cell, 40 μg/g of SMM induced 22.0+-5 SCE/cell and 380 μg/g of sulphonate-ethylmethane induced 8.6+-1.2 SCE/cell. This showed that all the agents were capable of inducing SCE in the bone marrow cells of rats in vivo under our conditions. We noted a greater induced efficiency for gamma radiation than the obtained by other investigators and a relatively similar efficiency in the case of chemical mutagens as reported in other studies. (author)

  13. Trans-generational radiation-induced chromosomal instability in the female enhances the action of chemical mutagens

    Energy Technology Data Exchange (ETDEWEB)

    Camats, Nuria [Institut de Biotecnologia i Biomedicina (IBB), Universitat Autonoma de Barcelona, 08193 Barcelona (Spain); Departament de Biologia Cel.lular, Fisiologia i Immunologia, Universitat Autonoma de Barcelona, 08193 Barcelona (Spain); Garcia, Francisca [Institut de Biotecnologia i Biomedicina (IBB), Universitat Autonoma de Barcelona, 08193 Barcelona (Spain); Parrilla, Juan Jose [Servicio de Ginecologia y Obstetricia, Hospital Universitario Virgen de la Arrixaca, 30120 El Palmar, Murcia (Spain); Calaf, Joaquim [Servei de Ginecologia i Obstetricia, Hospital Universitari de la Santa Creu i Sant Pau, 08025 Barcelona (Spain); Martin, Miguel [Departament de Pediatria, d' Obstetricia i Ginecologia i de Medicina Preventiva, Universitat Autonoma de Barcelona, 08193 Barcelona (Spain); Caldes, Montserrat Garcia [Institut de Biotecnologia i Biomedicina (IBB), Universitat Autonoma de Barcelona, 08193 Barcelona (Spain); Departament de Biologia Cel.lular, Fisiologia i Immunologia, Universitat Autonoma de Barcelona, 08193 Barcelona (Spain)], E-mail: Montserrat.Garcia.Caldes@uab.es

    2008-04-02

    Genomic instability can be produced by ionising radiation, so-called radiation-induced genomic instability, and chemical mutagens. Radiation-induced genomic instability occurs in both germinal and somatic cells and also in the offspring of irradiated individuals, and it is characterised by genetic changes including chromosomal rearrangements. The majority of studies of trans-generational, radiation-induced genomic instability have been described in the male germ line, whereas the authors who have chosen the female as a model are scarce. The aim of this work is to find out the radiation-induced effects in the foetal offspring of X-ray-treated female rats and, at the same time, the possible impact of this radiation-induced genomic instability on the action of a chemical mutagen. In order to achieve both goals, the quantity and quality of chromosomal damage were analysed. In order to detect trans-generational genomic instability, a total of 4806 metaphases from foetal tissues from the foetal offspring of X-irradiated female rats (5 Gy, acute dose) were analysed. The study's results showed that there is radiation-induced genomic instability: the number of aberrant metaphases and the breaks per total metaphases studied increased and were found to be statistically significant (p {<=} 0.05), with regard to the control group. In order to identify how this trans-generational, radiation-induced chromosomal instability could influence the chromosomal behaviour of the offspring of irradiated rat females in front of a chemical agent (aphidicolin), a total of 2481 metaphases were studied. The observed results showed that there is an enhancement of the action of the chemical agent: chromosomal breaks per aberrant metaphases show significant differences (p {<=} 0.05) in the X-ray- and aphidicolin-treated group as regards the aphidicolin-treated group. In conclusion, our findings indicate that there is trans-generational, radiation-induced chromosomal instability in the foetal

  14. Trans-generational radiation-induced chromosomal instability in the female enhances the action of chemical mutagens

    International Nuclear Information System (INIS)

    Genomic instability can be produced by ionising radiation, so-called radiation-induced genomic instability, and chemical mutagens. Radiation-induced genomic instability occurs in both germinal and somatic cells and also in the offspring of irradiated individuals, and it is characterised by genetic changes including chromosomal rearrangements. The majority of studies of trans-generational, radiation-induced genomic instability have been described in the male germ line, whereas the authors who have chosen the female as a model are scarce. The aim of this work is to find out the radiation-induced effects in the foetal offspring of X-ray-treated female rats and, at the same time, the possible impact of this radiation-induced genomic instability on the action of a chemical mutagen. In order to achieve both goals, the quantity and quality of chromosomal damage were analysed. In order to detect trans-generational genomic instability, a total of 4806 metaphases from foetal tissues from the foetal offspring of X-irradiated female rats (5 Gy, acute dose) were analysed. The study's results showed that there is radiation-induced genomic instability: the number of aberrant metaphases and the breaks per total metaphases studied increased and were found to be statistically significant (p ≤ 0.05), with regard to the control group. In order to identify how this trans-generational, radiation-induced chromosomal instability could influence the chromosomal behaviour of the offspring of irradiated rat females in front of a chemical agent (aphidicolin), a total of 2481 metaphases were studied. The observed results showed that there is an enhancement of the action of the chemical agent: chromosomal breaks per aberrant metaphases show significant differences (p ≤ 0.05) in the X-ray- and aphidicolin-treated group as regards the aphidicolin-treated group. In conclusion, our findings indicate that there is trans-generational, radiation-induced chromosomal instability in the foetal cells

  15. Mutagenic effects of Pingyangmycin on rice (Oryza sativa L.)

    International Nuclear Information System (INIS)

    The mutagenic effects of Pingyangmycin (PYM) on rice were studied by means of seed treatment with 5, 10, 15, 20 and 25 μg/ml of PYM. The results showed that PYM could induce high frequency of chromosome aberrations, produce physiological damages of various degrees in terms of seed germination, seedling growth and spikelet fertility in M1 generation, and induce high frequency of chlorophyll-deficient mutation and mutation in plant height and heading date in M2 generation. With 15 μg/ml of treatment, the highest total mutation frequency, higher than that of the widely used chemical mutagen EMS, was reached. As induction of beneficial mutations was concerned, PYM was inferior to EMS in inducing early heading mutation, but superior to EMS in inducing short straw mutation

  16. Mutation induction in mungbean, blackgram, chickpea and lentil using chemical mutagens

    International Nuclear Information System (INIS)

    Full text: Grain legumes cultivated in Bangladesh have narrow genetic bases. Seeds of four species were treated with sodium azide (NaN3) and EMS to create genetic variability. Phenotypically deviant types were selected in M2. The mungbean mutants were synchronous, early, bushy, erect and disease tolerant. Maximum frequency of variants occurred in the treatment with 0.75 mM of NaN3. The blackgram mutants were dwarf, bushy, trailing, synchronous and prolific podded. 1.0 mM NaN3 and 2% EMS concentrations produced the highest frequency of mutants. The chickpea mutants included broad-leaved, white flowered, erect, dwarf, bushy, early and chlorophyll-deficient types. White-flower mutants were reasonably free from wilt disease. The 0.4 mM concentration of NaN3 produced the highest frequency of mutants. In lentil, late flowering mutants were predominant. Some plants with increased number of pods were selected. Maximum frequency of mutants were obtained from 0.50 mM concentration of NaN3. (author)

  17. Some factors determining the concentration of liver proteins for optimal mutagenicity of chemicals in the Salmonella/microsome assay.

    Science.gov (United States)

    Malaveille, C; Kuroki, T; Brun, G; Hautefeuille, A; Camus, A M; Bartsch, H

    1979-12-01

    In plate assays in the presence of S. typhimurium TA100 and various amounts of liver 9000 X g supernatant (S9) from either untreated, phenobarbitone- (PB) or Aroclor-treated rats, the S9 concentration required for optimal mutagenicity of aflatoxin B1 (AFB) depended both on the source of S9 and on the concentration of the test compound. In these assays, the water-soluble procarcinogen, dimethylnitrosamine (DMN) was mutagenic in S. typhimurium TA1530 only in the presence of a 35-fold higher concentration of liver S9 from PB-treated rats than that required for AFB, a lipophilic compound. In liquid assays, a biphasic relationship was observed in the mutagenicities in S. typhimurium TA100 of benzo[a]pyrene (BP) and AFB and the concentration of liver S9. For optimal mutagenesis of BP, the concentration of liver S9 from rats treated with methylcholanthrene (MC) was 4.4% (v/v); for AFB it was 2.2% (v/v) liver S9 from either Aroclor-treated or untreated rats. At higher concentrations of S9 the mutagenicity of BP and of AFB was related inversely to the amount of S9 per assay. The effect of Aroclor treatment on the microsomemediated mutagenicity of AFB was assay-dependent: in the liquid assay, AFB mutagenicity was decreased, whereas in the plate assay it did not change or was increased. As virtually no bacteria-bound microsomes were detected by electron microscopy, after the bacteria had been incubated in a medium containing 1-34% (v/v) MC-treated rat-liver S9, it is concluded that, in mutagenicity assays, mutagenic metabolites generated by microsomal enzymes from certain pro-carcinogens have to diffuse through the assay medium before reaching the bacteria. Thus the mutagenicity of BP was dependent on both the concentration of rat-liver microsomes and that of total cytosolic proteins and other soluble nucleophiles such as glutathione. At a concentration of 4.4% (v/v) liver S9, the mutagenicity of BP was about 3.6 times higher than in assays containing a 4-fold higher

  18. Histological and genetic studies on the male sterile mutants of tomato induced by gamma radiation and chemical mutagens

    International Nuclear Information System (INIS)

    The object of this experiment was to test the radiosensitivity of the M1 generation, and to determine the inheritance and morphological characteristics of the male-sterile mutants in tomatoes. The results obtained are as follows: Radiation damage varied with the water content of the seeds. The least damage occurred in the range of 11.34 to 14.29 percent moisture. Four tomato cultivars were gamma irradiated. The cultivar Sekai-ichi was the most sensitive while the other responded similarly to each other. Chemical mutagen treatments caused greater damage to germination, to delayed flowering and to pollen fertility than did radiation. Pollen fertility and seeds per fruit in seedling gamma irradiated plants decreased as the dosage and dose rate increased. Five male-sterile mutants were found. Each was controlled by a single recessive gene. All five male-steriles were nonallelic. Floral sized of the male-sterile mutants were slightly smaller. Ovule fertility varied, usually being lower than normal type. Male sterility varied, usually being lower than normal type. Male sterility in 109-1ms strain was caused by unbalanced, insufficient nutrients being made available to the pollen-mother cells from the tapetal tissued. (author)

  19. Lycopersicon assays of chemical/radiation genotoxicity for the study of environmental mutagens.

    Science.gov (United States)

    Grant, William F; Owens, Elizabeth T

    2002-07-01

    From a literature survey, 21 chemicals are tabulated that have been evaluated in 39 assays for their clastogenic effects in Lycopersicon. Nineteen of the 21 chemicals are reported as giving a positive reaction (i.e. causing chromosome aberrations). Of these, five are reported positive with a dose response. In addition, 23 assays have been recorded for six types of radiation, all of which reacted positively. The results of 102 assays with 32 chemicals and seven types of radiation tested for the induction of gene mutations are tabulated, as well as 20 chemicals and/or radiation in combined treatments. The Lycopersicon esculentum (2n=24) assay is a very good plant bioassay for assessing chromosome damage both in mitosis and meiosis and for somatic mutations induced by chemicals and radiations. The Lycopersicon bioassay has been shown to be as sensitive and as specific an assay as other plant genotoxicity assays, such as Hordeum vulgare, Vicia faba, Crepis capillaris, Pisum sativum and Allium cepa and should be considered in further studies in assessing clastogenicity. Tests using L. esculentum can be made for a spectrum of mutant phenotypes of which many are identifiable in young seedlings. PMID:12088718

  20. The influence of mutagenic agents on rice seeds

    International Nuclear Information System (INIS)

    Investigation on the influence of the physical and chemical mutagenic, gamma rays, ethyl methane sulphonate (EMS) and dimethyl sulphonate (DMS) to oryza sativa var Pelita I/1 Rajalele, and Pelopor is done. Morphological observation on M-1, shows that the percentage of germination is high with the low dose, and the percentage decreases with the increase of dosage. The total production tillers per treated plants are generally higher than the controls. (author)

  1. Studying the synergistic damage effects induced by 1.8 GHz radiofrequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro

    International Nuclear Information System (INIS)

    The aim of this investigation was to study the synergistic DNA damage effects in human lymphocytes induced by 1.8 GHz radiofrequency field radiation (RFR, SAR of 3 W/kg) with four chemical mutagens, i.e. mitomycin C (MMC, DNA crosslinker), bleomycin (BLM, radiomimetic agent), methyl methanesulfonate (MMS, alkylating agent), and 4-nitroquinoline-1-oxide (4NQO, UV-mimetic agent). The DNA damage of lymphocytes exposed to RFR and/or with chemical mutagens was detected at two incubation time (0 or 21 h) after treatment with comet assay in vitro. Three combinative exposure ways were used. Cells were exposed to RFR and chemical mutagens for 2 and 3 h, respectively. Tail length (TL) and tail moment (TM) were utilized as DNA damage indexes. The results showed no difference of DNA damage indexes between RFR group and control group at 0 and 21 h incubation after exposure (P > 0.05). There were significant difference of DNA damage indexes between MMC group and RFR + MMC co-exposure group at 0 and 21 h incubation after treatment (P 0.05). The experimental results indicated 1.8 GHz RFR (SAR, 3 W/kg) for 2 h did not induce the human lymphocyte DNA damage effects in vitro, but could enhance the human lymphocyte DNA damage effects induced by MMC and 4NQO. The synergistic DNA damage effects of 1.8 GHz RFR with BLM or MMS were not obvious

  2. Mutations induction on the characters of wheat by physical and chemical mutagens

    International Nuclear Information System (INIS)

    Physical and chemical mutagenesis induces variability on genetical materials and after selectivity can be obtained mutants with improved and positive features. Induced mutations were produced from ionizing radiation sources and chemical agents. Throughout several years were obtained improved features materials, comparing with parents. In the wheat C 178 from physical mutagenesis, was obtained the line MF/3 by consecutive radiations: 15 kR and 10 kR. This line had a plant height of 25 % shorter than parent and better production features. After an experimental work and field tests in the years 1988-1996 the line MK3 4/3(LBZxL1376) treated wth dMS 0.004M present a new cultivar with these characteristics: protein content 11.6 %, yield 6 % higher than control and good agronomic type. (authors)

  3. Targeting of chemical mutagens to differentiating B-lymphocytes in vivo: detection by direct DNA labeling and sister chromatid exchange induction

    Energy Technology Data Exchange (ETDEWEB)

    Bloom, S.E.; Nanna, U.C.; Dietert, R.R.

    1987-01-01

    In vivo systems for analyzing mutagen interactions with a specific differentiating cell population are rare. Taking advantage of the unique anatomical features of the bursa of Fabricius in the chicken, the authors explored the possibility of targeting chemical mutagens to a defined differentiating cell population in the animal, namely, the B-lymphocytes series. Such cells are known to be the targets for the oncogene-activating avian leukosis virus. Targeting of chemicals to cells of the bursa was demonstrated by application of the DNA-specific fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to the anal lips of neonatal chicks. Bright nuclear fluorescence of cells in the bursa demonstrated to occur within minutes after the application of 500..mu..l of DAPI. DAPI labeling of nuclei was detected up to several days after a single application. No nuclear labeling was exhibited in cells of neighboring tissues. Methyl methanesulfonate (MMS)(10..mu..l) was applied to the anal lips of day-old chicks to study dose-response kinetics for mutagen targeting to DNA of dividing B-lymphocytes in the bursa. Since the mitotic index was found to be quite high (25-30%) in the bursa, chromosome analysis was used to assay for genome damage. Sister chromatid exchange frequencies of 3.9, 7.3, and 9.0 (baseline 2.5) per cell were obtained at MMS dosages per animal of 50 ..mu..g, 100..mu..g, and 200..mu..g, respectively. These results indicate the rapid and quantitative localization of DNA-binding chemicals to cells of the bursa, particularly the resident B-lymphocytes. The bursa should be a useful system for studying mutagen-DNA interactions in the differentiating B-lymphocyte and subsequent influences on the development of immunity and lymphoproliferative disease.

  4. Targeting of chemical mutagens to differentiating B-lymphocytes in vivo: detection by direct DNA labeling and sister chromatid exchange induction

    International Nuclear Information System (INIS)

    In vivo systems for analyzing mutagen interactions with a specific differentiating cell population are rare. Taking advantage of the unique anatomical features of the bursa of Fabricius in the chicken, the authors explored the possibility of targeting chemical mutagens to a defined differentiating cell population in the animal, namely, the B-lymphocytes series. Such cells are known to be the targets for the oncogene-activating avian leukosis virus. Targeting of chemicals to cells of the bursa was demonstrated by application of the DNA-specific fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to the anal lips of neonatal chicks. Bright nuclear fluorescence of cells in the bursa demonstrated to occur within minutes after the application of 500μl of DAPI. DAPI labeling of nuclei was detected up to several days after a single application. No nuclear labeling was exhibited in cells of neighboring tissues. Methyl methanesulfonate (MMS)(10μl) was applied to the anal lips of day-old chicks to study dose-response kinetics for mutagen targeting to DNA of dividing B-lymphocytes in the bursa. Since the mitotic index was found to be quite high (25-30%) in the bursa, chromosome analysis was used to assay for genome damage. Sister chromatid exchange frequencies of 3.9, 7.3, and 9.0 (baseline 2.5) per cell were obtained at MMS dosages per animal of 50 μg, 100μg, and 200μg, respectively. These results indicate the rapid and quantitative localization of DNA-binding chemicals to cells of the bursa, particularly the resident B-lymphocytes. The bursa should be a useful system for studying mutagen-DNA interactions in the differentiating B-lymphocyte and subsequent influences on the development of immunity and lymphoproliferative disease

  5. Effect of chemical mutagens and carcinogens on gene expression profiles in human TK6 cells.

    Directory of Open Access Journals (Sweden)

    Lode Godderis

    Full Text Available Characterization of toxicogenomic signatures of carcinogen exposure holds significant promise for mechanistic and predictive toxicology. In vitro transcriptomic studies allow the comparison of the response to chemicals with diverse mode of actions under controlled experimental conditions. We conducted an in vitro study in TK6 cells to characterize gene expression signatures of exposure to 15 genotoxic carcinogens frequently used in European industries. We also examined the dose-responsive changes in gene expression, and perturbation of biochemical pathways in response to these carcinogens. TK6 cells were exposed at 3 dose levels for 24 h with and without S9 human metabolic mix. Since S9 had an impact on gene expression (885 genes, we analyzed the gene expression data from cells cultures incubated with S9 and without S9 independently. The ribosome pathway was affected by all chemical-dose combinations. However in general, no similar gene expression was observed among carcinogens. Further, pathways, i.e. cell cycle, DNA repair mechanisms, RNA degradation, that were common within sets of chemical-dose combination were suggested by clustergram. Linear trends in dose-response of gene expression were observed for Trichloroethylene, Benz[a]anthracene, Epichlorohydrin, Benzene, and Hydroquinone. The significantly altered genes were involved in the regulation of (anti- apoptosis, maintenance of cell survival, tumor necrosis factor-related pathways and immune response, in agreement with several other studies. Similarly in S9+ cultures, Benz[a]pyrene, Styrene and Trichloroethylene each modified over 1000 genes at high concentrations. Our findings expand our understanding of the transcriptomic response to genotoxic carcinogens, revealing the alteration of diverse sets of genes and pathways involved in cellular homeostasis and cell cycle control.

  6. Rhizobium japonicum mutants induced by gamma rays and some chemical mutagens

    International Nuclear Information System (INIS)

    Numerous symbiotic mutants of R.japonicum were isolated after UV irradiation or nitrosoguanidine mutagensis. Cultures of R.japonicum GM 377 were irradiated with 0, 25, 50, 75, 100, 150, 175, 200, 250 and 300 krad of gamma-rays. As for chemical mutagensis, cultures were treated with Sodium azide and colchicin at concentration of 1x10-6, 1x10-5, 5x10-5, 1x10-4, 5x10-4, 1x10-3 and 5x10-3 mol. Results revealed that gentamicin at concentration of 24.0 Mg/ml was able to kill all the populations of R.japonicum. Therefore, this concentration was used for mutants selection. However, numerous mutants at different freguencies were identified. The highest percentage of mutant was obtained with Sodium azide at 1x10-4 mol

  7. Proceedings of the 8th workshop on plant mutation breeding. Effective use of physical/chemical mutagens

    Energy Technology Data Exchange (ETDEWEB)

    Kume, Tamikazu; Watanabe, Kazuo; Tano, Shigemitsu (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment

    2001-03-01

    The Workshop on Plant Mutation Breeding of FNCA (Forum for Nuclear Cooperation in Asia), was held on 9-13 October 2000 in Hanoi, Vietnam. The Workshop was co-sponsored by the Science and Technology Agency (STA), the Ministry of Science, Technology and Environment (MOSTE of Vietnam) and the Ministry of Agriculture and Rural Development (MARD of Vietnam) in cooperation with the Ministry of Agriculture, Forestry and Fisheries (MAFF), National Institute of Agrobiological Resources (NIAR of Vietnam), the Japan Atomic Industrial Forum (JAIF) and Japan Atomic Energy Research Institute (JAERI). Two Scientists, a Project Leader and an expert on methodology for plant/crop mutation breeding, participated from each of the member countries, i.e. China, Indonesia, Malaysia, the Philippines, Thailand, Vietnam and Japan. Also attending the Workshop were, one participant from Korea, seven participants from both Japan and Vietnam. The number of the participants in the Workshop totalled about sixty people including guests and observers. Sixteen papers including eight invited papers on the current status of methodology for plant/crop mutation breeding in the participating countries were presented. Discussions were focused on the subject concerning 'Effective Use of Physical/Chemical Mutagens', as well as a detailed report on the current status of research in each participating country. In addition, the topics of developing a mutant breeding database, an information exchange for plant/crop mutation breeding, and more tightly bound international co-operative research in the near future were also high on the agenda. This proceeding compiles the invited and contributed papers that were submitted from the speakers. (author)

  8. Proceedings of the 8th workshop on plant mutation breeding. Effective use of physical/chemical mutagens

    International Nuclear Information System (INIS)

    The Workshop on Plant Mutation Breeding of FNCA (Forum for Nuclear Cooperation in Asia), was held on 9-13 October 2000 in Hanoi, Vietnam. The Workshop was co-sponsored by the Science and Technology Agency (STA), the Ministry of Science, Technology and Environment (MOSTE of Vietnam) and the Ministry of Agriculture and Rural Development (MARD of Vietnam) in cooperation with the Ministry of Agriculture, Forestry and Fisheries (MAFF), National Institute of Agrobiological Resources (NIAR of Vietnam), the Japan Atomic Industrial Forum (JAIF) and Japan Atomic Energy Research Institute (JAERI). Two Scientists, a Project Leader and an expert on methodology for plant/crop mutation breeding, participated from each of the member countries, i.e. China, Indonesia, Malaysia, the Philippines, Thailand, Vietnam and Japan. Also attending the Workshop were, one participant from Korea, seven participants from both Japan and Vietnam. The number of the participants in the Workshop totalled about sixty people including guests and observers. Sixteen papers including eight invited papers on the current status of methodology for plant/crop mutation breeding in the participating countries were presented. Discussions were focused on the subject concerning 'Effective Use of Physical/Chemical Mutagens', as well as a detailed report on the current status of research in each participating country. In addition, the topics of developing a mutant breeding database, an information exchange for plant/crop mutation breeding, and more tightly bound international co-operative research in the near future were also high on the agenda. This proceeding compiles the invited and contributed papers that were submitted from the speakers. (author)

  9. Protection against genetic hazards from environmental chemical mutagens: experience with ionizing radiation

    International Nuclear Information System (INIS)

    In radiation protection, the recurrent theme is, and always has been, dose limitation whether it is for occupational workers, individual members of the public or the population as a whole. The key words are 'dose' and 'limitation'. The quantitative system of dose limitation has been achieved because of a number of conceptual developments in our understanding of the mechanism of radiation action, development of radiation dosimetry, the accumulation of a vast body of quantitative information on dose-effect relationships and the effects of various biological and physical variables that affect these relationships of data on patterns and levels of exposures likely to be encountered to make estimates of the effects expected to result from such exposures, and balancing of risks to society against the benefits derived, the latter a matter of informed judgement. The philosophy has always been to avoid all unnecessary exposures and to limit the necessary exposures (justified by the benefits expected) to as low a level as reasonably achievable, social and economic factors being taken into acccount. The introduction of the concept that the system of dose limitation to the population should be based on genetic risks has stressed the need for careful planning to ensure that our genetic heritage is not endangered. Transfer of this knowledge to the field of chemical protection is discussed. (Auth.)

  10. 59. Protectivc effect of melatonin on genetic damage by chemical mutagen and the influence on cell prolife-ration kenetics

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: In this study, we observed the effect of melatonin on the frequency of sister chromatid exchange, micronucleus formation of binuclear cell in lymphocyte from human peripheral blood in vitro, micronucleus formation of mouse bone marrow polycychromatic erythrocyte in vivo, which were induced by chemical mutagen, and lymphocyte proliferation kenetics in vitro. Methods: ① Lymphocytes were cultured in vitro in the presence of 0.01,0.10,1.00 mmol/L melatonin, mitomycin C(MMC) (positive control), 0.5% ethanol (negative control)and 0.01,0.10,1.00 mmol/L melatonin plus MMC for 72 h at 37℃±1℃. Lymphocytes were examined for the frequence of SCE, mitotic index, cell proliferation cycle, cell cycle ratio and proliferation index. ② Lymphocytes were cultured in vitro in the presence of 0.01,0.10,1.00 mmol/L melatonin, mitomycin C(MMC) (positive control), 0.5% ethanol (negative control) and 0.01,0.10,1.00 mmol/L melatonin plus MMC for 44 h at 37℃±1℃. Then each culture was given cytochalasin B, which was cultured to 72 h. Binuclear lymphocytes were examined for the micronucleus rate. ③ The mice were administered with 0.1, 1.0,10.0 mg/kg*bw melatonin and distillated water (negative control) respectively for 7 d, then were given melatonin plus cyclophosphamide (CP) (positive control) for 2 d since the eighth day. The rate of micronulclei of mouse bone marrow polycychromatic erythrocyte was examined. Results: ① The frequences of sister chromatid exchange of lymphocytes which were cultured in the presence of 0.01,0.10,1.00 mmol/L melatonin compared with negative control exhibited no statistical significance. ② The SCE of cells treated with melatonin plus MMC compared with positive control were markedly decreased. ③ The mitotic indices of lymphocytes cultured in the presence of 0.10,1.00 mmol/L melatonin were lower than negative control. The proliferation index was significant lower than negative control only in the culture exposed to 1.00 mmol

  11. Chemical Profiles and Identification of Key Compound Caffeine in Marine-Derived Traditional Chinese Medicine <em>Ostreae em>concha>

    Directory of Open Access Journals (Sweden)

    Hong-Bing Liu

    2012-05-01

    Full Text Available To compare the chemical differences between the medicinal and<em> em>cultured oyster shells, their chemical profiles were investigated. Using the ultra performance liquid chromatography-electron spraying ionization-mass spectrometry (UPLC-ESI-MS, combined with principal component analysis (PCA and orthogonal projection to latent structures discriminant analysis (OPLS-DA, the discrimination of the chemical characteristics among the medicinal and cultured oyster shells was established. Moreover, the chemometric analysis revealed some potential key compounds. After a large-scale extraction and isolation, one target key compound was unambiguously identified as caffeine (1 based on extensive spectroscopic data analysis (1D and 2D NMR, MS, and UV and comparison with literature data.

  12. Chemical Composition, Antimicrobial and Antitumor Activities of the Essential Oils and Crude Extracts of<em> Euphorbiaem>> macrorrhizaem>

    Directory of Open Access Journals (Sweden)

    Haji Akber Aisa

    2012-05-01

    Full Text Available The present study aimed to examine the chemical composition and biological activity of essential oils extracted from <em>Euphorbia macrorrhizaem> collected from Northwest China. The major constituents of the essential oils of aerial parts and roots of <em>E. macrorrhizaem> are acorenone B (16.72% and 25.80%, (+-cycloisosativene (14.94% and 12.40%, 3a-hydroxy-5b-androstane (10.62% and 5.52%, copaene (7.37% and 6.29%, l-calamenene (4.13% and 4.65% and β-cedrene (8.40% and 7.98%, respectively. The minor components of them are thymene, γ-terpinene, thymecamphor, α-cedrene, zingiberene, trans-caryophyllene, β-chamigrene, curcumene, pentadecane, (−-α-muurolene, cuparene, γ-cadinene, (<em>Z>-3-heptadecene, 1,3,7,7-tetramethyl-2-oxabicyclo(4.4.0dec-5-en-4-one, hexahydrofarnesyl acetone, γ-elixene and palmitinic acid. The antimicrobial and antitumor activitiy of the <em>E. macrorrhizaem> essential oil against <em>Staphyloccocus aureusem>, <em>Escherichia coliem>, <em>Canidia Albicansem> and Caco-2 cells were evaluated. Among all the tested microorganisms and Caco-2 cells, the essential oils showed the strongest inhibitory effect on <em>Staphyloccocus aureus em>(MIC = 2.8 μg/mL and Caco-2 cell (IC50 = 11.86 μg/mL, whereas no effect on <em>Escherichia coliem> and <em>Candida em>albicans>. The data of this study suggested that the <em>E. macrorrhizaem> essential oils have great potential as a natural medicine for microbial infections and cancers.

  13. Mutation induction in mangosteen : Response of explants to mutagens

    OpenAIRE

    Phrommee, V.; Te-chato, S.

    1999-01-01

    Young purplish red leaves of mangosteen raised in two-phase medium and calli were collected and treated with two mutagens, gamma rays and ethylethane sulfonate (EMS). After treating with both mutagens, leaves and calli were cultured onto callus induction medium to evaluate the effect of mutagens on percent leaf forming callus and survival percentage of callus. The results showed that an increament of EMS caused decreased in survival rate of calli. The concentration that inhibited 50%growth of...

  14. A robust method for assessing chemically induced mutagenic effects in the oral cavity of transgenic Big Blue® rats.

    Science.gov (United States)

    Young, Robert R; Thompson, Chad M; Dinesdurage, Harshini R; Elbekai, Reem H; Suh, Mina; Rohr, Annette C; Proctor, Deborah M

    2015-08-01

    The Big Blue® (BB) in vivo mutation assay uses transgenic rodents to measure treatment-induced mutations in virtually any tissue. The BB assay can be conducted in rats or mice and is ideal for investigating tissue-specific mutagenic mode of action of tumor induction. Some tissues such as oral mucosa have not been thoroughly studied. Due to the small quantity and cartilaginous nature of oral cavity tissues, development of special prosection and DNA isolation methods was required to permit robust analysis of mutations in these tissues. Improved surgical methods permitted collection of adequate and reproducible quantities of tissue (∼45 mg gingiva/buccal and ∼30 mg gingiva/palate). Optimized DNA isolation methods included use of liquid nitrogen pulverization, homogenization, nuclei pelleting, digestion, and phenol/chloroform extraction, to yield sufficient quantities of DNA from these tissues. In preliminary optimization work, mutant frequency (MF) in tongue and gingiva was increased in rats exposed to the promutagen, benzo[a]pyrene, and the direct mutagen, N-ethyl-N-nitrosourea. The oral cavity carcinogen, 4-nitroquinoline-1-oxide (4-NQO; 10 ppm in drinking water; 28 days), was qualified as a positive control for mutagenesis in oral tissues since it caused significant increases in cII MFs in gingiva/palate (50.2-fold) and gingiva/buccal tissues (21.3-fold), but not in liver or bone marrow (0.9- and 1.4-fold, respectively). These results are consistent with the observation that 4-NQO primarily induces tumors in oral cavity. Results also demonstrate the utility of the BB rat mutation assay and optimized methods for investigation of oral cavity mutagenicity, and by extension, analysis of other small and cartilaginous tissues. PMID:25969955

  15. Reduction of hexavalent chromium by fasted and fed human gastric fluid. I. Chemical reduction and mitigation of mutagenicity.

    Science.gov (United States)

    De Flora, Silvio; Camoirano, Anna; Micale, Rosanna T; La Maestra, Sebastiano; Savarino, Vincenzo; Zentilin, Patrizia; Marabotto, Elisa; Suh, Mina; Proctor, Deborah M

    2016-09-01

    Evaluation of the reducing capacity of human gastric fluid from healthy individuals, under fasted and fed conditions, is critical for assessing the cancer hazard posed by ingested hexavalent chromium [Cr(VI)] and for developing quantitative physiologically-based pharmacokinetic models used in risk assessment. In the present study, the patterns of Cr(VI) reduction were evaluated in 16 paired pre- and post-meal gastric fluid samples collected from 8 healthy volunteers. Human gastric fluid was effective both in reducing Cr(VI), as measured by using the s-diphenylcarbazide colorimetric method, and in attenuating mutagenicity in the Ames test. The mean (±SE) Cr(VI)-reducing ability of post-meal samples (20.4±2.6μgCr(VI)/mL gastric fluid) was significantly higher than that of pre-meal samples (10.2±2.3μgCr(VI)/mL gastric fluid). When using the mutagenicity assay, the decrease of mutagenicity produced by pre-meal and post-meal samples corresponded to reduction of 13.3±1.9 and 25.6±2.8μgCr(VI)/mL gastric fluid, respectively. These data are comparable to parallel results conducted by using speciated isotope dilution mass spectrometry. Cr(VI) reduction was rapid, with >70% of total reduction occurring within 1min and 98% of reduction is achieved within 30min with post-meal gastric fluid at pH2.0. pH dependence was observed with decreasing Cr(VI) reducing capacity at higher pH. Attenuation of the mutagenic response is consistent with the lack of DNA damage observed in the gastrointestinal tract of rodents following administration of ≤180ppm Cr(VI) for up to 90days in drinking water. Quantifying Cr(VI) reduction kinetics in the human gastrointestinal tract is necessary for assessing the potential hazards posed by Cr(VI) in drinking water. PMID:27404458

  16. The Role of Genotypes That Modify the Toxicity of Chemical Mutagens in the Risk for Myeloproliferative Neoplasms

    Directory of Open Access Journals (Sweden)

    Carol Ann Gross-Davis

    2015-02-01

    Full Text Available Background: The etiology of myeloproliferative neoplasms (MPN (polycythemia vera; essential thrombocythemia; primary myelofibrosis is unknown, however they are associated with a somatic mutation—JAK2 V617F—suggesting a potential role for environmental mutagens. Methods: We conducted a population-based case-control study in three rural Pennsylvania counties of persons born 1921–1968 and residing in the area between 2000–2008. Twenty seven MPN cases and 292 controls were recruited through random digit dialing. Subjects were genotyped and odds ratios estimated for a select set of polymorphisms in environmentally sensitive genes that might implicate specific environmental mutagens if found to be associated with a disease. Results: The presence of NAT2 slow acetylator genotype, and CYP1A2, GSTA1, and GSTM3 variants were associated with an average 3–5 fold increased risk. Conclusions: Exposures, such as to aromatic compounds, whose toxicity is modified by genotypes associated with outcome in our analysis may play a role in the environmental etiology of MPNs.

  17. Chemical Composition and Biological Activities of the Essential Oils from <em>Duguetia lanceolataem> St. Hil. Barks

    Directory of Open Access Journals (Sweden)

    Maria A. C. Kaplan

    2012-09-01

    Full Text Available Essential oils of <em>Duguetia lanceolataem> barks, obtained at 2 (T2 and 4 h (T4, were identified by gas chromatography and gas chromatography/mass spectrometry. β-Elemene (12.7 and 14.9%, caryophyllene oxide (12.4 and 10.7% and β-selinene (8.4 and 10.4% were the most abundant components in T2 and T4, respectively. The essential oils inhibited the growth of <em>Staphylococcus aureusem>,> Streptococcus pyogenesem>, <em>Escherichia coliem> and <em>Candida albicansem>. The essential oils were cytotoxic against brine shrimp. The extraction time influenced the chemical composition and biological activities of essential oils obtained from the barks of <em>D. lanceolataem>.

  18. Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells

    Science.gov (United States)

    Perrin, Anne; Freire, Maëlle; Bachelet, Christine; Collin, Alice; Levêque, Philippe; Pla, Simon; Debouzy, Jean-Claude

    2010-11-01

    We investigated the effect of a 1800 MHz radiofrequency GSM signal combined with a known chemical mutagen (4-nitroquinoline-N-oxide: 4NQO) on human THP1 cells. Comet and γ-H2AX assays were used to assess DNA damage. No heating of the cell cultures was noted during exposure (2 h). The exposure of cells to electromagnetic fields with SARs of 2 to 16 W/kg did not increase the DNA damage induced by 4NQO, whereas the number of DNA strand breaks increased with a temperature increase of at least 4 °C. In conclusion, no co-genotoxic effect of radiofrequency was found at levels of exposure that did not induce heating.

  19. Repair pathways in mammalian cells: Incision-ligation kinetics after UV, x-rays and mutagenic chemicals. Effects of 3-aminobenzamide and hydroxyurea + cytosine arabinoside

    International Nuclear Information System (INIS)

    Repair of DNA-damage involves the combined action of several enzyme functions. With regard to the incision step different pathways exist. In nucleotide excision repair an endonuclease recognizes the lesion, cuts the DNA strand and the faulty nucleotide is excised together with a number of undamaged nucleotides. This seems to be the process which operates in mammalian cells after exposure to UV and a number of chemicals which introduce so called bulky lesions in the DNA. After exposure to X-rays and simple alkylating chemicals such as methyl methanesulfonate. The first step involves the action of a glycosylase which only removes the damaged base. The incision is exercised by an endonuclease which recognises abasic sites. In this process, i.e. base excision repair, probably only the damaged nucleotide is exchanged. When the damaged piece of DNA has been removed one would imagine that, independently of the type of lesion initially present, the same types of enzymes could be used to close the gap in the strand, this seems, however, not to be the case. In this investigation the authors used 3-aminobenzamide (BA) or hydroxy urea (HU) combined with cytosine arabinoside (ara C) to study repair of damage induced by UV and a number of mutagenic chemicals. DNA strand breaks have been measured by the DNA-unwinding technique

  20. Development of an improved and early sowing potential variety of jute - binadeshipat-2 using chemical mutagen sodium azide

    International Nuclear Information System (INIS)

    Jute is the important agricultural and industrial resource of Bangladesh for earning foreign currency. Seeds of the widely cultivated variety, CVL-1 were treated with 4 mM to 20mM of sodium azide (NaN3). The treated seeds were grown in M1 to M10 generations. A single plant (selection number C-278) was selected from M2 segregating population on the basis of early sowing potential and late flowering. Ten fiber quality characters were tested and it was found that the fiber quality of the mutant had been improved. Hence, the mutagen sodium azide showed extra potentiality to induce fiber quality characters of jute. The strain C-278 was also tested through Preliminary, Advanced, Zonal Yield Trial and Farmers field trial at different locations of the jute growing area in Bangladesh. The field evaluation team of the National Seed Board (NSB) visited most of the trials and reported that at early sowing and overall performance of the strain C-278 was found best than that of the mother variety. Besides these the strain produced 7.5% and 20% higher fiber yield than the mother variety CVL-1 and a local cultivar, D-154, respectively. After evaluation of the Technical Committee of NSB recommended that the strain C-278 may be released as a variety. The National Seed Board released the strain C-278 as a National jute variety under the name Binadeshipat-2 for cultivation in Bangladesh

  1. Biological effects of mutagenic agents

    International Nuclear Information System (INIS)

    There is an increasing body of evidence that mutagenic agents (biological, chemical and physical) play an important role in the etiology of human diseases. Mutations may occur in the germinal as well as in the somatic cells. Mutations of the germ cells may result on infertility or fertilization of damaged cells, the later leading to abortion or birth of a malformed fetus. Somatic-cells mutations may have various biological effects, depending on the period of the human life at which the mutation occurs. If it occurs during the prenatal life, a teratogenic or carcinogenic effect will be observed. If the somatic cell is damaged during the postnatal life, this will lead to neoplastic transformation. Therefore it is extremely important to know the mutagenic, teratogenic and carcinogenic effects of various biological, chemical and physical agents in order to eliminate them from our environment. (author). 13 refs, 4 figs, 1 tab

  2. Mutagenicity of alcoholic beverages.

    Science.gov (United States)

    Nagao, M; Takahashi, Y; Wakabayashi, K; Sugimura, T

    1981-02-01

    The mutagenicities of evaporated residues of alcoholic beverages were tested by the Ames method with the modification of pre-incubation, by using Salmonella typhimurium TA100 and TA98. 12 of 13 brands of whisky were mutagenic to TA100 without S9 mix. Addition of S9 mix decreased or abolished these mutagenicities. 5 brands of brandy and 1 apple brandy were tested, and all showed a similar type of mutagenicity to that of whisky. A fraction of brand-K whisky, containing a major mutagen(s), eluted from XAD-2 column with water, gave 3800 revertants of TA100 per plate at a dose equivalent to 10 ml of whisky. PMID:7012607

  3. Cytotoxicity and mutagenicity of cola and grape flavored soft drinks in bone marrow cells of rodents Citotoxicidade e mutagenicidade de refrigerantes sabor cola e uva, em células de medula óssea de roedor

    Directory of Open Access Journals (Sweden)

    Elisângela Düsman

    2013-03-01

    Full Text Available Due to the large consumption of soft drinks in Brazil and worldwide in recent years and considering that some of the components present in their composition pose potential risks to human health, the aim of this study was to evaluate the cytotoxic and mutagenic potential of specific cola and grape-flavored soft drink brands. Bone marrow cells of Wistar rats were initially treated by gavage with one single dose of Cola or Grape soft drink, which was next offered ad libitum (instead of water for 24 hours. A negative control treatment was performed by administering one single dose of water and a positive control administering cyclophosphamide intraperitoneally. Statistical analysis showed that the Cola and Grape soft drinks studied were not cytotoxic. However, the Cola soft drink proved mutagenic in this experiment treatment time. Therefore, this study serves as a warning about the consumption of Cola-flavored soft drink and for the need for further subchronic and chronic studies on soft drinks in order to evaluate the long term mutagenic and cytotoxic effects of these substances.Devido ao grande consumo de refrigerantes no Brasil e no mundo nos últimos anos, e tendo em vista que alguns dos componentes presentes na composição destes possuem potenciais danosos para os organismos, em especial o humano, o objetivo deste trabalho foi avaliar o potencial citotóxico e mutagênico de uma marca de refrigerante sabor Cola e uma de sabor Uva. Foram utilizadas como sistema-teste as células de medula óssea de ratos Wistar, tratados via gavagem com dose única do refrigerante sabor Cola ou Uva e, em seguida, fornecidos ad libitum (no lugar da água, por 24 horas. Foi feito um controle negativo, administrando água, em dose única, e um controle positivo administrando ciclofosfamida, via intraperitoneal. A análise estatística mostrou que os refrigerantes sabor Cola e Uva não foram citotóxicos. Entretanto, o refrigerante sabor Cola foi mutagênico neste

  4. Bacterial mutagenicity assays: test methods.

    Science.gov (United States)

    Gatehouse, David

    2012-01-01

    The most widely used assays for detecting chemically induced gene mutations are those employing bacteria. The plate incorporation assay using various Salmonella typhimurium LT2 and E. coli WP2 strains is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances capable of causing DNA damage leading to gene mutations. The test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs.The test uses several strains of S. typhimurium which carry different mutations in various genes of the histidine operon, and E. coli which carry the same AT base pair at the critical mutation site within the trpE gene. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. When these auxotrophic bacterial strains are grown on a minimal media agar plates containing a trace of the required amino-acid (histidine or tryptophan), only those bacteria that revert to amino-acid independence (His(+) or Tryp(+)) will grow to form visible colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner.This chapter provides detailed procedures for performing the test in the presence and absence of a metabolic activation system (S9-mix), including advice on specific assay variations and any technical problems. PMID:22147566

  5. Mutagenicity potential of commercial broth cubes at varying concentrations

    International Nuclear Information System (INIS)

    Today, there has been a growing concern on the mutagenicity potential of environmental chemical systems. These environmental chemicals such as pesticides, food additives, synthetic drugs, water and atmospheric pollutants are possible causes of mutagenic activity. Meat products and some meat flavorings, were also reported to exhibit mutagenic activity. And since these products are normal part of the daily human diet, there is a need for extensive studies regarding the possible mutagenic activity associated with these products. This study aimed to evaluate the mutagenicity potential of commercial broth cubes at varying concentration. The researchers sought to answer the following questions: 1. Do beef, pork and chicken broth cubes exhibit mutagenic activity? 2. Are there significant differences in the mutagenic activity among the three samples? 3. Are these significant differences in the mutagenic activity exhibited by each of the samples compared to that of Mitomycin-C (positive control)? 4. Which of the sample of each specific concentration exhibit the greatest mutagenic activity? Three specific concentrations of beef, pork and chicken broth cubes were prepared and their mutagenicity potential was evaluated by using the Micronucleus test. The formation of micro nucleated polychromatic and micro nucleated normo chromatic erythrocytes in bone marrow cells of mice treated with these samples were detected using a Carl-Zeiss photo microscope. The statistical tool used to test the validity of the null hypothesis was analysis of variance using randomized complete block design and independent T- test. (author)

  6. MUTAGENICITY AND GENOTOXICITY OF SUPERFICIAL AND GROUNDWATER BEFORE AND AFTER WATER TREATMENT = MUTAGENICIDADE E GENOTOXICIDADE EM ÁGUAS SUPERFICIAIS E SUBTERRÂNEAS ANTES E APÓS O TRATAMENTO DA ÁGUA

    Directory of Open Access Journals (Sweden)

    Regina Teresa Rosim Monteiro

    2013-01-01

    Full Text Available Water disinfection is a millenarian process; nevertheless, there are few studies about the safe use of chemicals for the consumer population. It is known that chlorine can react with the organic matter present in the water and form trihalomethanes (TAMs. Exposure to chlorinated water, which occurs by food, bathing, recreation or other uses have been the cause for concern due the damage caused to health, being bladder cancer the most worrisome. To evaluate the formation of genetic material damage, such as chromosome breaks, bridges, and others that cause loss of genetic material during cell division, genotoxicity and mutagenicity tests was performed using Allium cepa in samples of chlorinated drinking water. For the tests, samples of natural and treated water on four different water treatment stations from the city of Piracicaba, SP were used. The frequencies of chromosomal aberrations (AC and micronucleus (MN were analyzed after exposure of seeds, in three periods of 2008. Significant values of toxicity were found for samples of untreated and treated water compared to control. In water samples from a region that is predominantly agricultural, there was greater toxicity after treatment. It is suggested that these values are probably derived from products used in agriculture, transported to water bodies, increasing the organic load and the possibility of formation of TAMs, due to the use of chlorine in treated water samples. These results emphasize the possibility of formation of carcinogenic compounds during water treatment. = A desinfecção da água é um processo milenar, apesar disso, há poucos estudos sobre a segurança do uso de compostos químicos para a população consumidora. Sabe-se que o cloro, reagindo com a matéria orgânica presente na água, pode formar trihalometanos (TAMs. A exposição às águas cloradas como alimento, banho, recreação ou outros usos têm sido causa de preocupação devido aos danos à saúde, sendo o c

  7. Use of chemical mutagen for induction of useful genetic variation in different oil seed rape (brassica napus L.) cultivars

    International Nuclear Information System (INIS)

    Pre soaked seeds for 16 hrs of oil seed Rape cvs. Tower and Wester were treated with 1-3% concentrations of ethylene methane sulphonate (EMS) for 4 hrs with the objective to generate variation in characters of importance such as, early maturity, semi dwarf stature, lodging resistance and different yield components. After post washing (4 hrs) the seeds were filter dried and planted directly in the field as M1. Selection for desirable mutants was carried out in M2 and a number of mutants with desirable traits were selected during 1990-91. The mutants were tested for stability of the selected traits in plant to progenies in M3/M4 during 1992 and 1993. The stable 17 mutants were evaluated for yield and other agronomic characters in M5, in preliminary yield trials, during 1993-94. Eleven mutants exhibited early maturity and high yield potential than parent and local commercial variety. The promising mutants will be further tested in yield trials in future for confirmation of results. (author)

  8. Comparison of the mutagenicities of fossil fuels.

    Science.gov (United States)

    Pelroy, R A; Sklarew, D S; Downey, S P

    1981-11-01

    Chemical fractions from 4 shale oils including one produced in an above-ground retort (Paraho), 2 produced below ground by in situ method (Occidental and Geokinetics), and 1 produced by a simulated modified in situ process (Livermore), were tested for mutagenicity against Salmonella typhimurium TA98 in the Ames assay system. Their mutagenic activities were compared with 2 other complex materials: a high-boiling coal distillate (The Solvent Refined Coal Process I (SRC II) heavy distillate), and a crude petroleum (Prudhoe Bay). Each material was fractionated by Sephadex LH-20 partition chromatography and by acid-base solvent extraction to provide chemical fractions for bioassay. Mutagenic activity was detected in the coal liquid (and their fractions), but not in the natural crude oil nor in any of its fractions. The basic and tar fractions derived by solvent extraction, and the LH-20 methanol fractions contained most of the mutagenic activity recovered from the unfractionated material. The heavy distillate was approx. 10 times more active in the Ames assay than the most mutagenically active shale oil. The most mutagenically active of the shale oils was about 10 times more active than the least active shale oil. PMID:7035938

  9. Characterization of the Antibacterial Activity and the Chemical Components of the Volatile Oil of the Leaves of <em>Rubus parvifoliusem> L.

    Directory of Open Access Journals (Sweden)

    Yongqing Cai

    2012-06-01

    Full Text Available <em>Rubus parvifoliusem> L. (Rp is a medicinal herb that possesses antibacterial activity. In this study, we extracted the volatile oil from the leaves of Rp to assess its antibacterial activity and analyze its chemical composition. A uniform distribution design was used to optimize the extraction procedure, which yielded 0.36% (<em>w/w> of light yellowish oil from the water extract of Rp leaves. We found that the extracted oil effectively inhibited the growth of a wide range of Gram positive and negative bacteria, including <em>Staphylococcus aureusem>, <em>Staphylococcus epidermidisem>, <em>Enterococcus faecalisem>, <em>Escherichia coliem>, <em>Pseudomonas aeruginosaem>, <em>Acinetobacter baumaniiem>, <em>Bacillus cloacaeem>, and <em>Klebsiella pneumoniaeem>. We further analyzed the components contained in the hydro-distillated Rp volatile oil by gas chromatography-mass spectroscopy. Twenty nine compounds were identified, including 4-hydroxy-3-methoxystyrene (66%, 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10% and 4-<em>tert>-butylbenzoic acid (2%. Our results suggest that one or multiple constituents contained in Rp volatile oil may account for its antibacterial activity.

  10. Mutagenic interactions between near-ultraviolet (365 nm) radiation and alkylating agents in Escherichia coli

    International Nuclear Information System (INIS)

    The mutagenic interaction between near-ultrviolet (365 nm) radiation and the alkylting agents ehtyl methanesulponate (EMS) and methyl methanesulphonate (MMS) was studied in a repair-component and an excision-deficient stram of Escherichia coli. Near-UV raditation modified the metabolic response of of exposure to these chemicals and either reduced or increased their mutagenic efficiency. Based on these results, an experimental model was formulated to explain the mutagenic interactions that occur between near-UV and various agents that induce prototrophic reverants cia error-prone repair of DNA. According to this model, low doses of near-UV provoke conditions for mutation frequency decline (MFI) and lead to a mutagenic antagonism. With increasing near-Uv doses, damage to constitutive error-free repairs system increases, favouring the error-prone system and inhibiting the MFD. Under these conditions there will be a progressive decrease in antagonism until at high doses an enhancement of mutation frequency (positive interaction) will occur. (orig.)

  11. Influence of Chemical Extraction Conditions on the Physicochemical and Functional Properties of Polysaccharide Gum from Durian (<em>Durio> <em>zibethinus> Seed

    Directory of Open Access Journals (Sweden)

    Bahareh Tabatabaee Amid

    2012-05-01

    Full Text Available Durian seed is an agricultural biomass waste of durian fruit. It can be a natural plant source of non-starch polysaccharide gum with potential functional properties. The main goal of the present study was to investigate the effect of chemical extraction variables (<em>i.e>., the decolouring time, soaking temperature and soaking time on the physicochemical properties of durian seed gum. The physicochemical and functional properties of chemically-extracted durian seed gum were assessed by determining the particle size and distribution, solubility and the water- and oil-holding capacity (WHC and OHC. The present work revealed that the soaking time should be considered as the most critical extraction variable affecting the physicochemical properties of crude durian seed gum.

  12. 浸种温度与化学诱变剂对芝麻种子发芽的影响%Effects of Steeping Temperature and Chemical Mutagen on Seeds Germination of Sesame

    Institute of Scientific and Technical Information of China (English)

    刘艳阳; 梅鸿献; 武轲; 郑永战; 张海洋

    2012-01-01

    In order to study the effects of steeping temperature and chemical mutagen on seeds germination of sesame,sesame seeds were pre-soaked in water under 4 ℃ and 25 ℃ for 24 h,and the impact of different concentrations and treatment time of EMS and NaN3 on the germination potential,germination rate,germination index and vigor index of sesame were studied. The results showed that the three sesame varieties pre-soaked in water under 4 ℃ could not germinate normally in the treatment of EMS for more than 4 h or beyond 10 g/L, while those pre-soaked in water under 25 ℃ could germinate normally by treatment with 10 g/L of EMS for 6 h or 15 g/L of EMS for 4 h. In the treatment of NaN3, three sesame varieties could germinate normally after pre-soaked at both of degrees. Notably, the germination rate of sesame seeds pre-soaked under 4 ℃ was less than 50. 0% by treatment with 6 mmol/L of NaN3 for 12 h,while the three sesame varieties had a germination rate above 69. 3% after pre-soaked under 25 ℃. When sesame seeds were pre-soaked in water under 4 ℃ , EMS and NaN3, in general, played better roles under the same treating combination of concentration and time duration. In addition, the sensitivity to the EMS varied greatly among different sesame varieties,while they had a close sensitivity to NaN3. In order to obtain the desirable mutations,it is recommended to select the application of 5 g/L of EMS for 8 h or 4 mmol/L of NaN3 for 12 h after the seeds pre-soaked in water under 4 ℃ for 24 h.%为了探讨浸种温度及化学诱变剂对芝麻种子发芽的影响,在不同浸种温度下,研究了甲基磺酸乙酯(EMS)和叠氮化钠(NaN3)不同(质量)浓度处理不同时间对芝麻种子发芽势、发芽率、发芽指数、活力指数的影响.结果表明,4℃浸种后,EMS处理时间超过4h或者EMS质量浓度大于10 g/L时,3个芝麻品种均不能正常发芽,而25℃浸种后,10 g/L EMS处理6h或15 g/L EMS处理4h,3个品种仍能正常发芽.4

  13. Effect of mutagens on seed germination in chilli (Capsicum annuum L.)

    International Nuclear Information System (INIS)

    Seeds of chilli variety CO-2 (Coimbatore-2) were irradiated with gamma rays ranging from 10 kR to 35 kR at an interval of 5 kR and the effect on seed germination, seedling survival, percent lethality and seedling injury were studied. Lower doses were stimulative, while higher doses had inhibitory effect on seed germination and seedling survival. The highest percentage of seed germination (37.5) and seedling survival (31.3) was recorded at 10 kR as compared to 28 percent of germination and 3.3 percent seedling survival in control. Percent lethality (9.6%) and seedling injury (6.5%) were comparatively low than the lethality percentage and seedling injury of the higher dose treatments. In chemical mutagen treatments, the maximum seed germination (54.5%) and seedling survival (51.2%), seedling lethality (0.97%) and seedling injury (1.37%) were obtained in the treated seeds of EMS at 0.5% concentration followed by 39.5% seed germination and 30.0% seedling survival, seedling lethality (3.8%) and seedling injury (3.06%) of MMS. The stimulative effect of seed germination is more in chemical mutagens than the physical mutagen. There was a proportionate decrease in germination percentage and seedling survival with an increase in dose/concentration of both the chemicals. (author)

  14. Physical-chemical and microbiological characterization, and mutagenic activity of airborne PM sampled in a biomass-fueled electrical production facility.

    Science.gov (United States)

    Cohn, Corey A; Lemieux, Christine L; Long, Alexandra S; Kystol, Jørgen; Vogel, Ulla; White, Paul A; Madsen, Anne Mette

    2011-05-01

    Biomass combustion is used in heating and electric power generation in many areas of the world. Airborne particulate matter (PM) is released when biomass is brought to a facility, stored, and combusted. Occupational exposure to airborne PM within biomass-fueled facilities may lead to health problems. In March and August of 2006, airborne PM was collected from a biomass-fueled facility located in Denmark. In addition, source-specific PM was generated from straw and wood pellets using a rotating drum. The PM was analyzed for polycyclic aromatic hydrocarbons (PAHs), metals, microbial components, mutagenic activity, and ability to generate highly reactive oxygen species (hROS) in cell-free aqueous suspensions. PM collected from the boiler room and the biomass storage hall had higher levels of mutagenic activity, PAHs and metals, and a higher hROS generating potential than the source specific PM. The mutagenic activity was generally more potent without S9 activation, and on the metabolically enhanced strain YG1041, relative to TA98. Significant correlations were found between mutagenicity on YG1041 (without S9) and PAH concentration and mutagenicity on YG1041 (with S9) and hROS generating ability. PM collected in March was more toxic than PM collected in August. Overall, airborne PM collected from the facility, especially that from the boiler room, were more toxic than PM generated from straw and wood chips. The results suggest that exposure to combustion PM in a biomass-fueled facility, which likely includes PM from biomass combustion as well as internal combustion vehicles, may contribute to an elevated risk of adverse health effects. PMID:20872826

  15. Utilization of Mutagenic Treatments for Improving Barley Disease Resistance to Powdery Mildew

    International Nuclear Information System (INIS)

    This program aims to improve barley (Hordeum vulgar L.) resistance to powdery mildew (Erysiphe graminis f. sp. hordi) using mutation induction technique via physical and chemical mutagens. Grains of two local cultivars were treated with gamma rays (150, 250 GY) at a dose rate of 9.02 Rad/ Sec alone or with the concentrations of chemical mutagens, i.e.,EMS (4 and 8 mM), MH (10 and 20 mM) for two hours. After mass selection for the resistance in M2, the two cultivars were varied in their responses to the fifteen treatments. In M3 generation, the frequencies of plant resistance were increased at 20 mM of MH, 8 mM of EMS or 250 GY with either of them for the cultivar G124. However, the same trend was also found at 10 mM of MH, 250GY alone and with 4 or 8 mM EMS for cultivar G125. Moreover, eight mutants were selected from the progenies of M4 plants and evaluated in M5 generation. These mutants are characterized by highly disease resistance and high yield components, one of them has been characterized by non crytics and awnless .The desirable mutants should go to further evaluation for grain yield and grain quality in subsequent generations. (author)

  16. Chemical Composition of <em>Artemisia annuaem> L. Leaves and Antioxidant Potential of Extracts as a Function of Extraction Solvents

    OpenAIRE

    Maznah Ismail; Shahid Iqbal; Umer Younas; Kim Wei Chan; Muhammad Zia-Ul-Haq

    2012-01-01

    This study was conducted to investigate the chemical and nutritional composition of <em>Artemisia annuaem> leaves in addition to determination of antioxidant potential of their extracts prepared in different solvents. Chemical composition was determined by quantifying fat, protein, carbohydrate, fiber, tocopherol, phytate, and tannin contents. Extraction of <em>A. annuaem> leaves, for antioxidant potential evaluation, was carried out using five solvents of differen...

  17. Physical‐chemical and microbiological characterization, and mutagenic activity of airborne PM sampled in a biomass‐fueled electrical production facility

    DEFF Research Database (Denmark)

    Cohn, Corey A.; Lemieux, Christine L.; Long, Alexandra S.;

    2011-01-01

    Biomass combustion is used in heating and electric power generation in many areas of the world. Airborne particulate matter (PM) is released when biomass is brought to a facility, stored, and combusted. Occupational exposure to airborne PM within biomass‐fueled facilities may lead to health...... problems. In March and August of 2006, airborne PM was collected from a biomass‐fueled facility located in Denmark. In addition, source‐specific PM was generated from straw and wood pellets using a rotating drum. The PM was analyzed for polycyclic aromatic hydrocarbons (PAHs), metals, microbial components......, mutagenic activity, and ability to generate highly reactive oxygen species (hROS) in cell‐free aqueous suspensions. PM collected from the boiler room and the biomass storage hall had higher levels of mutagenic activity, PAHs and metals, and a higher hROS generating potential than the source specific PM. The...

  18. Molecular and genetic mechanisms of environmental mutagens

    International Nuclear Information System (INIS)

    This program is primarily concerned with elucidation of the nature of DNA lesions produced by environmental and energy related mutagens, their mechanisms of action, and their repair. The main focus is on actions of chemical mutagens and electromagnetic radiations. Synergistic interactions between mutagens and the mutational processes that lead to synergism are being investigated. Mutagens are chosen for study on the basis of their potential for analysis of mutation (as genetic probes), for development of procedures for reducing mutational damage, for their potential importance to risk assessment, and for development of improved mutagen testing systems. Bacterial cells are used because of the rapidity and clarity of scientific results that can be obtained, the detailed genetic maps, and the many well-defined mutand strains available. The conventional tools of microbial and molecular genetics are used, along with intercomparison of genetically related strains. Advantage is taken of tcollective dose commitment will result in more attention being paid to potential releases of radionuclides at relatively short times after disposal

  19. Avaliação da mutagênese e genotoxicidade em Eigenmannia virescens (Teleostei: Gymnotiformes expostos ao benzeno Avaliation of mutagenicity and gentotoxicity in Eigenmannia virescens (Teleostei: Gymnotiformes exposed to benzene

    Directory of Open Access Journals (Sweden)

    Augusto Bücker

    2006-01-01

    Full Text Available Os efeitos de substâncias genotóxicas sobre o genoma de peixes tem sido objeto de muitos estudos, sobretudo daqueles que buscam estabelecer a resposta dos genes aos estímulos ambientais. O presente trabalho teve como objetivo realizar um estudo sobre mutagenicidade e genotoxicidade em peixes elétricos da espécie Eingenmannia virescens, pela exposição ao benzeno (50ppm, utilizando as técnicas da Freqüência de Micronúcleos (MNs e o Ensaio do Cometa. Foram coletadas amostras do sangue de dez peixes em diferentes tempos de exposição: T0, 24h, 48h, 72h, 96h e 360h (15 dias. Para a análise das lâminas no Teste do MN, foram contadas 1.000 células e estipulada a freqüência de ocorrência de MNs. Para análise do Ensaio do Cometa a contagem foi feita estipulando quatro classes de danos: I - II - III - IV, e para a análise estatística foram atribuídos valores numéricos (ranques de 0 a 3, respectivamente, verificando diferenças significativas para a soma dos ranques em todos os tempos de exposição em relação ao T0. No Teste do Micronúcleo não foi possível detectar efeitos mutagênicos significativos nos eritrócitos analisados. No entanto, para o Ensaio do Cometa os resultados sugerem ação genotóxica do benzeno, devido a um aumento gradual no número de células com maiores classes de danos de acordo com maior tempo de exposição, indicando um efeito tempo-dependente. Estes resultados sugerem maior sensibilidade do Ensaio do Cometa que o Teste do MN.The effects of genotoxic substances on fishes genome have been object of many studies, especially those which attempt to establish the responses of genes to environmental stimulus. The objective of this study was to evaluate the mutagenicity and genotoxicity in electric fishes of species Eingenmannia virescens, under to benzene exposure (50ppm, utilizing Micronuclei Test (MNs and Comet Assay techniques. Ten fish blood samples were collected in different times of exposure: T0

  20. Scanning electron-microscopic and X-ray-microanalytic observation of diesel-emission particles associated with mutagenicity

    International Nuclear Information System (INIS)

    The particles formed by diesel combustion, which may contain various mutagenic chemicals like polycyclic aromatic hydrocarbons (PAH), are analyzed in their morphology by scanning electron microscopy; their sulfur content is detected by X-ray microanalysis, and mutagenicity is tested with a Salmonella typhimurium bioassay. The authors find a close correlation between sulfur content and mutagenicity of PAH. (Auth.)

  1. Avaliação da mutagenicidade e antimutagenicidade de um biopolímero extraído do microorganismo Agrobacterium radiobacter em camundongos Swiss Assessment of mutagenicity and antimutagenicity of a biopolymer extracted from the microorganism Agrobacterium radiobacter in mice

    Directory of Open Access Journals (Sweden)

    Milka Selestina Primo

    2010-07-01

    Full Text Available A presente pesquisa avaliou a ação mutagênica e antimutagênica de um biopolímero de glucose extraído da Agrobacterium radiobacter (Biopolímero de Agrobacterium radiobacter. O experimento foi realizado com camundongos Swiss machos divididos em oito grupos. O tratamento com o biopolímero foi realizado por gavage em dose única concomitante a uma dose de solução tampão fosfato nos grupos de avaliação da mutagenicidade, ou ao agente indutor de danos no DNA, ciclofosfamida, na concentração de 50 mg/kg (peso corpóreo - p.c., nos grupos de avaliação da antimutagenicidade. Utilizou-se o teste de micronúcleo em sangue periférico e a coleta de sangue foi realizada 24 e 48 h após a aplicação das substâncias-teste. A análise estatística demonstrou que o biopolímero não possui atividade mutagênica e que é efetivo em prevenir danos no DNA. As porcentagens de redução de danos nos grupos de antimutagenicidade foram de 83,9%, 89,1% e 103,1% em 24 h e 101,24%, 98,14% e 120,64% em 48 h para as doses de 75, 150 e 300mg/kg (p.c., respectivamente. A alta porcentagem de redução de danos associada à ausência de efeitos mutagênicos indica, além da atividade quimioprotetora, a possibilidade do biopolímero ser um alimento funcional candidato à utilização como co-adjuvante na quimioterapia para prevenir efeitos colaterais.This study evaluated the mutagenic and ant mutagenic action of a biopolymer of glucose extracted from Agrobacterium radiobacter (Biopolymer of Agrobacterium radiobacter. The experiment was conducted with Swiss male mice divided into eight groups. Treatment with the biopolymer was performed in a single dose by gavage at a dose of concomitant phosphate buffer groups in the evaluation of mutagenicity, or the agent of inducing DNA damage, cyclophosphamide, the concentration of 50 mg/kg (body weight --b.w., in groups of assessment ant mutagenic. We used the micronucleus test in peripheral blood. The blood sample was

  2. Relative effectiveness of mutagens on amylase production in Aspergillus wentii

    International Nuclear Information System (INIS)

    Three physical mutagens (UV light and γ- and X-rays) and 3 chemical mutagens (N-methyl-N-nitro-N-nitrosoguanidine, hydroxylamine and nitrous acid) were tested for their ability to produce mutants of A. wentii Wehmer (IMI 17295) having altered amylase activity. There was marked variation in amylase activity among the mutants, increase, decrease or complete absence of such activity was noticed. Nitrous acid was most effective in inducing high yielding amylase producing mutants. (author)

  3. Assessment of the Mutagenicity of Sediments from Yangtze River Estuary Using Salmonella Typhimurium/Microsome Assay.

    Directory of Open Access Journals (Sweden)

    Li Liu

    Full Text Available Sediments in estuaries are of important environmental concern because they may act as pollution sinks and sources to the overlying water body. These sediments can be accumulated by benthic organisms. This study assessed the mutagenic potential of sediment extracts from the Yangtze River estuary by using the Ames fluctuation assay with the Salmonella typhimurium his (- strain TA98 (frameshift mutagen indicator and TA100 (baseshift mutagen indicator. Most of the sediment samples were mutagenic to the strain TA98, regardless of the presence or absence of exogenous metabolic activation (S9 induction by β-naphthoflavone/phenobarbital. However, none of the samples were mutagenic to the strain TA100. Thus, the mutagenicity pattern was mainly frameshift mutation, and the responsible toxicants were both direct (without S9 mix and indirect (with S9 mix mutagens. The mutagenicity of the sediment extracts increased when S9 was added. Chemical analysis showed a poor correlation between the content of priority polycyclic aromatic hydrocarbons and the detected mutagenicity in each sample. The concept of effect-directed analysis was used to analyze possible compounds responsible for the detected mutagenic effects. With regard to the mutagenicity of sediment fractions, non-polar compounds as well as weakly and moderately polar compounds played a main role. Further investigations should be conducted to identify the responsible components.

  4. Enhanced production of protease by mutagenized strain of aspergillus oryzae in solid substrate fermentation of rice bran

    International Nuclear Information System (INIS)

    Neutral protease activity of parent strain of Aspergellus oryzae was enhanced by UV and chemical mutagenization with ethyl methane sulphonate (EMS). After screening, a hyper producing strain was isolated and found effective for tile production of neutral protease as compared to the parent strain of Aspergellus oryzae. Solid substrate fermentation was carried out in 250ml conical flask with 45 % initial moisture contents at a temperature of 30 deg. C for 72 flours. Under the optimum conditions maximum yield of neutral protease obtained was 662.61+-0.36 U/gds, Almost all the organic nitrogen supplements favored the enzyme production while sucrose proved as a best carbon source. (author)

  5. Chemical Composition and <em>in Vitroem> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii em>Essential Oil and Extracts<em>>

    Directory of Open Access Journals (Sweden)

    François Couderc

    2012-08-01

    Full Text Available In this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water of <em>Eucalyptus giliiem> were screened for their chemical composition, antimicrobial and antioxidant activities. The essential oil chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS and gas chromatography-flame ionization detection (GC-FID, respectively. Thirty four compounds were identified, corresponding to 99.5% of the total essential oil. Tannins [104.9–251.3 g catechin equivalent (CE/Kg dry mass], flavonoids [3.3–34.3 g quercetin equivalent (QE/Kg dry mass], phenolics [4.7–216.6 g gallic acid equivalent (GAE/Kg dry mass] and anthocyannins [1.2–45.3 mg cyanidin-3-glucoside equivalent (C3GE/Kg dry mass] of various extracts were investigated. Free radical scavenging capacity of all samples was determinedt. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH assay, the IC50 of essential oil was 163.5 ± 10.7 mg/L and in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS assay, it was 94.7 ± 7.1 mg/L. Among the various extracts, the water extract showed the best result (IC50 = 11.4 ± 0.6 mg/L in the DPPH assay which was comparable to vitamin C (IC50 = 4.4 ± 0.2 mg/L. The antimicrobial activities were evaluated against different bacterial and fungal strains. Gram positive bacteria were found to be more sensitive to the essential oil and extracts than Gram negative ones. Anthocyanins seem to have a major effect on the growth of <em>Bacillus subtilisem> (R2 = 0.79. A significant antifungal activity was observed against the yeast and fungi. Correlations between chemical composition and antioxidant activities were studied and R2 values were about 0.96 for the effect of phenolics on the DPPH assay.

  6. Mutagenic and chemical analyses provide new insight into enzyme activation and mechanism of the type 2 iron-sulfur l-serine dehydratase from Legionella pneumophila.

    Science.gov (United States)

    Xu, Xiao Lan; Grant, Gregory A

    2016-04-15

    The crystal structure of the Type 2 l-serine dehydratase from Legionella pneumophila (lpLSD), revealed a "tail-in-mouth" configuration where the C-terminal residue acts as an intrinsic competitive inhibitor. This pre-catalytic structure undergoes an activation step prior to catalytic turnover. Mutagenic analysis of residues at or near the active site cleft is consistent with stabilization of substrate binding by many of the same residues that interact with the C-terminal cysteine and highlight the critical role of certain tail residues in activity. pH-rate profiles show that a residue with pK of 5.9 must be deprotonated and a residue with a pK of 8.5 must be protonated for activity. This supports an earlier suggestion that His 61 is the likely catalytic base. An additional residue with a pK of 8.5-9 increases cooperativity when it is deprotonated. This investigation also demonstrates that the Fe-S dehydratases convert the enamine/imine intermediates of the catalytic reaction to products on the enzyme prior to release. This is in contrast to pyridoxyl 5' phosphate based dehydratases that release an enamine/imine intermediate into solution, which then hydrolyzes to produce the ketoamine product. PMID:26971469

  7. Mutagenic Activity of Indigofera truxillensis and I. suffruticosa Aerial Parts

    Directory of Open Access Journals (Sweden)

    Tamara Regina Calvo

    2011-01-01

    Full Text Available Indigofera truxillensis and I. suffruticosa, are used as a source of indigo dye and to treat several diseases. The mutagenic activity of the methanolic extracts from aerial parts, glycerolipid, flavonoid and alkaloid fractions of the extract were evaluated by means of Salmonella/microsome assays using TA100, TA98, TA102 and TA97a strains. The methanolic extract of I. truxillensis showed mutagenic activity in the TA98 strain without S9 while glycerolipid fraction was devoid of activity. The flavonoid and alkaloid fractions of both plants showed mutagenicity. Chemical analysis of flavonoid fractions of I. truxillensis and I. suffruticosa resulted in the identification of kaempferol, quercetin and their derivatives. The alkaloid fraction of both the species contained indigo and indirubin and indigo was found mainly responsible for the mutagenic activity.

  8. Granuloma pouch assay for mutagenicity testing.

    Science.gov (United States)

    Maier, P

    1980-11-01

    The Granuloma Pouch Assay (GPA) is an animal model in which mutagenic and carcinogenic effects of a testcompound can be detected in rapidly dividing fibroblasts of a granulation tissue in adult male rats. Growth of this tissue was initiated with a small amount of croton oil at the inside wall of a subcutaneous air pouch on the back of the animals. The test compound can be injected either into the pouch (local) or administered by systemic routes. Alkali labile DNA-lesions, chromosome aberrations, sister chromatid exchanges, point mutations and tumor development in situ were determined. The comparison of mutation frequencies after local and systemic administration of testcompounds, provide an estimation of the pharmacokinetic characteristics and the mutagenic potency of the chemical. The local application route allows the detection of locally active mutagens and of compounds which require activation by P-448 dependent mono-oxygenases. Liver mediated proximate metabolites are detectable when they are transformed into ultimate carcinogens in extrahepatic cells whereas chemicals with a strong organ specific activity are not. PMID:7235991

  9. Mutation research with ionizing radiations and chemicals using drosophila: problems, results and perspectives

    International Nuclear Information System (INIS)

    The results of mutagenesis studies with Drosophila are examined in order to inquire the extent to which the genetic responses to ionizing radiations and to chemical mutagens share common features, to identify those findings that may have validity beyond the confines of this species, and to assess the contributions and implications of Drosophila results to the problems of mutagenicity testing and of the evaluation of genetic hazards to man from exposure to environmental chemicals. The results obtained in the studies permit the estimation of X-irradiation equivalent doses of EMS for the induction of sex-linked recessive lethals and specific locus mutations in mature spermatozoa. (Auth.)

  10. Mutagenic potential of water from rivers, lakes and seas; Mutagenes Potential von Wasser aus Fluessen, Seen und Meeren

    Energy Technology Data Exchange (ETDEWEB)

    Zietz, B. [Goettingen Univ. (Germany). Zentrum fuer Umwelt- und Arbeitsmedizin; Pfeiffer, E.H. [Universitaetskrankenhaus Eppendorf, Hamburg (Germany). Lehrstuhl fuer Hygiene

    2000-07-01

    Examinations of mutagenicity with the Salmonella/microsome test are reviewed that were carried out on surface waters. In surface waters mutagenicity could preferably be found after a concentration of the samples. Adsorption to XAD resins was used most frequently as a concentration method. It showed good results especially with large volumes of water. The extraction with organic solvents was performed with good results, too. Testing samples without concentration was tried only by some authors. Results of testing for the kind of mutagenicity were very heterogeneous. In surface waters frameshift mutagens that only take effect after metabolic transformation were most abundant. In the next rank of frequency were direct acting frameshiftmutagens. Every second sampling place showed basepairsubstituting mutagens. Mutagens that only take effect after metabolic transformation were more frequent, too. Studies using concentration by adsorption to XAD resins had positive testings for mutagenicity at an amount of 10 to 1250 ml extracted water. Today chemical characterisation is often added to mutagenicity testing. On the basis of the reviewed articles it can be concluded that in regions with human influence a load of mutagens has to be expected in every sample of survace water. (orig.) [German] Es werden Mutagenitaetsuntersuchungen mit dem Salmonella/Mikrosomen-Test nach Ames dargestellt, die an Oberflaechenwaessern durchgefuehrt worden sind. Ein mutagenes Potential konnte bevorzugt nach einer Probenanreicherung nachgewiesen werden. Als Anreicherungsmethode wurde die Adsorption an XAD-Harz oder andere Adsorbentien weitaus am haeufigsten verwendet. Sie liess sich auch bei grossen Probenvolumina sehr gut handhaben. Ebenfalls gute Ergebnisse lieferte die Extraktion der Wasserproben mit organischen Loesungsmitteln. Das Testen von Proben ohne Anreicherung wurde eher selten versucht. Die Testergebnisse zur Art der Mutagenitaet der verschiedenen Waesser waren sehr heterogen. In

  11. Mutagenic activity and heterocyclic amine content of the human diet

    Energy Technology Data Exchange (ETDEWEB)

    Knize, M.G.; Dolbeare, F.A.; Cunningham, P.L.; Felton, J.S.

    1993-01-15

    The mutagenic activity and the mass amount of heterocyclic amines responsible for the mutagenic activity have been measured in some cooked foods. Cooked meats are the predominant source of mutagenic activity in the diet with values ranging from 0 to 10,000 revertants per gram reported in the Ames/Salmonelia test with strain TA98. Several heterocyclic amines are present and have been quantified using solid-phase extraction followed by HPLC. Frying at higher temperatures and for longer times produces the greatest mutagenic response, and concomitantly, the largest amounts of heterocyclic amines. Most of the mutagenic activity in fried meat samples can be accounted for by MelQx, DiMelQx and IQ, although other heterocylic amines are present and PHIP mutagenic activity becomes significant at higher temperatures. Non-meat products such as baked breads can also form significant mutagenic activity, particularly when overcooked. Commercially prepared hamburgers made from meat substitutes such as tofu, wheat gluten or tempeh and fried at 210{degrees}C have up to 10% of the mutagenic activity of a fried beef patty cooked under the same conditions. When detected, amounts of heterocyclic amines in fried beef patties range from a total of 0.35 ng/g for commercial beef hamburgers to 142 ng/g for a beef patty cooked over a barbecue. Dietary intake is expected to have a large range, from less than one microgram per day to over 50 micrograms per day based on current knowledge of known heterocyclic amine chemicals and heterocyclic amine-containing foods.

  12. Mutagenicity assessment of produced water during photoelectrocatalytic degradation.

    Science.gov (United States)

    Li, Guiying; An, Taicheng; Nie, Xiangping; Sheng, Guoying; Zeng, Xiangying; Fu, Jiamo; Lin, Zheng; Zeng, Eddy Y

    2007-03-01

    Oilfield produced water was treated by photocatalysis, electro-oxidation, and photoelectrocatalysis, respectively. The chemical composition and toxicity of the raw effluent and treated products were assessed by chemical and mutagenicity analysis. The raw effluent exhibited mutagenic activity in both strains of Salmonella typhimurium. The lowest concentration of the dichloromethane extract capable of inducing a positive response in strains TA98 and TA100 were as low as 4 and 5 microg/plate, respectively. All three technologies could detoxify direct-acting mutagenic organic pollutants efficiently, although they could not completely eliminate mutagenicity in the water after 60 min of treatment. At equivalent doses, photoelectrocatalysis exhibited the greatest capability to reduce genotoxicity, whereas photocatalysis was the least effective and did not cause appreciable change in mutagenicity. The gas chromatography-mass spectrometry (GC-MS) analysis revealed that n-alkanes (259.4 ng/L) and phenolic compounds (2,501.4 ng/L) were the main organic constituents in the oilfield produced water. Thus, the results of both biological and chemical analysis indicate that photoelectocatalysis was the most effective technology for degradation of oilfield wastewater. PMID:17373504

  13. Physical and chemical mutagenesis on a mycophagous nematode Aphelenchoides composticola (M.T. Franklin, 1957)

    International Nuclear Information System (INIS)

    Chemical mutagens as EMS, acriflavine, acridine, colchicine, nitrous acide and physical mutagens, such as X rays, have been used on the gonochoric mycophagous Nematode Aphelenchoides composticola. They show a nematicid activity due, to their toxicity on treated Nematodes and to the induction of lethal mutations affecting particularly early stages of gametogenesis. They produce abnormal strains dwarfs or giants (up to 25% of the population). Concentrations of chemical mutagens varying from 0.2 to 0.5% correspond to the optimal production of abnormalities. Similar results were obtained by irradiation near to 2000r. The action of the mutagens shows some differences: EMS and X rays generally produce dwarfs, whereas acriflavine, acridine, colchicine or nitrous acid induced only giants. Abnormal strains appear: in the F1, generation by X rays or acridine treatments; in the F2 or F3 generation by acriflavine, colchicine, nitrous acid or EMS action. The abnormal strains could be either variants or mutants and from these we select: four dwarfs B, C, D, E, induced by EMS 0.5% for 24 hours appearing in the F3 generation; or dwarf F induced by irradiation of 1500r appearing in the F1 generation. All these selected mutants are autosomal recessive single factors D and C controlled by two alleles of the some locus

  14. [The mutagenicity of caramel colors].

    Science.gov (United States)

    Scheutwinkel-Reich, M; von der Hude, W

    1985-12-01

    In the present study commercially available caustic and ammoniated caramel colours were tested for their mutagenic potential using the Ames assay. The test was performed using the standard test strains Salmonella typhimurium TA 1535, TA 1537, TA 98 and TA 100 with and without a metabolic activation system (S9-mix). Furthermore, a special preincubation procedure without metabolic activation system was applied. None of the tested caramel colours showed any mutagenic effect in the Ames test. PMID:4082782

  15. Entrepreneurship as Elixir and Mutagen

    OpenAIRE

    Lundmark, Erik; Westelius, Alf

    2014-01-01

    Metaphors are powerful tools for sensemaking, sensegiving, and theory development, but they are often concealed in academic writing. This paper uncovers two metaphors underlying entrepreneurship discourse and research—elixir and mutagen. The elixir metaphor is uncovered by examining critiques of entrepreneurship research, and serves as a compact description of problematic aspects entrepreneurship scholars should be mindful of. The mutagen metaphor is uncovered by examining evolutionary framew...

  16. Optimalization of mutagens doses in field bean (Vicia faba minor) breeding

    International Nuclear Information System (INIS)

    The article contains results of a laboratory experiment performed to determine the degree of stem and root length reduction in field bean seedlings grown from seeds treated with mutagens - physical (gamma-ray radiation and fast neutrons) and chemical ( N-nitroso-N-ethylurea - NEU and N-nitroso-N-methylurea - NMU). Among physical mutagens the largest reduction of stem and root length was caused by doses from 9 to 15 kR of gamma-ray radiation, while among chemical mutagens - by NEU at 0.050% and 0.075% concentration. All mutagens, except gamma-ray radiation, caused a larger reduction in root than in stem length. In most cases the degree of root and stem reduction increased with increasing mutagen dose. (author)

  17. Chemical Composition and Biological Activities of the Essential Oils from <em>Duguetia lanceolataem> St. Hil. Barks

    OpenAIRE

    Kaplan, Maria A. C.; Rodarte, Mírian P.; Maria P. H. Amaral; Araújo, Aílson A. L.; Míriam A. O. Pinto; Maria S. Alves; Glauciemar Del-Vechio-Vieira; Orlando V. Sousa

    2012-01-01

    Essential oils of <em>Duguetia lanceolataem> barks, obtained at 2 (T2) and 4 h (T4), were identified by gas chromatography and gas chromatography/mass spectrometry. β-Elemene (12.7 and 14.9%), caryophyllene oxide (12.4 and 10.7%) and β-selinene (8.4 and 10.4%) were the most abundant components in T2 and T4, respectively. The essential oils inhibited the growth of <em>Staphylococcus aureusem>,> Streptococcus pyogenesem>, <em>Escherichia coli&l...

  18. Genotoxicity of complex mixtures: CHO cell mutagenicity assay

    Energy Technology Data Exchange (ETDEWEB)

    Frazier, M.E.; Samuel, J.E.

    1985-02-01

    A Chinese hamster ovary (CHO) mammalian cell assay was used to evaluate the genotoxicity of complex mixtures (synthetic fuels). The genotoxicity (mutagenic potency) of the mixtures increased as the temperature of their boiling range increased. Most of the genotoxicity in the 750/sup 0/F+ boiling-range materials was associated with the neutral polycyclic aromatic hydrocarbon (PAH) fractions. Chemical analysis data indicate that the PAH fractions of high-boiling coal liquids contain a number of known chemical carcinogens, including five- and six-ring polyaromatics (e.g., benzo(a)pyrene) as well as four- and five-ring alkyl-substituted PAH (e.g., methylchrysene and dimethylbenzanthracenes); concentrations are a function of boiling point (bp). In vitro genotoxicity was also detected in fractions of nitrogen-containing polyaromatic compounds, as well as in those with aliphatics of hydroxy-containing PAH. Mutagenic activity of some fractions was detectable in the CHO assay in the absence of an exogenous metabolic activation system; in some instances, addition of exogenous enzymes and cofactors inhibited expression of the direct-acting mutagenic potential of the fraction. These data indicate that the organic matrix of the chemical fraction determines whether, and to what degree, various mutagens are expressed in the CHO assay. Therefore, the results of biological assays of these mixtures must be correlated with chemical analyses for proper interpretation of these data. 29 references, 16 figures, 4 tables.

  19. DETECTION OF CARCINOGENICITY BASED ON MUTAGENICITY IN ARABIDOPSIS

    Science.gov (United States)

    Thirty-seven synthetic chemicals plus two mycotoxins were tested for mutagenicity in an Arabidopsis embryo system. The results of this test, prokaryotic repair tests, bacterial mutation assays, eukaryotic cell systems, and in vivo tests were compared to the carcinogenicity classi...

  20. mutagenic treatments and selection in chickpea

    International Nuclear Information System (INIS)

    the present study was carried out through the three subsequent winter seasons of 1980/1981,1981/1982 and 1982/1983 at the experimental research center, faculty of agriculture , university of cairo, at giza and at the experimental farm belonging to the egyptian energy establishment, inshas. this investigation included two experiments . the first experiment was conducted to study the effect of gamma rays and Ems. on yield and yield components of four genotypes of chickpea. the second experiment was designed to study the effect of selection through the mutagenic treatments.the materials used consisted of four chickpea genotypes, three of them (i.e giza 1, Nec 1055 and Nec 1046) are kabuli type and the fourth (Family 88) is desi type

  1. Chemical Investigation of Saponins in Different Parts of <em>Panax notoginseng em>by Pressurized Liquid Extraction and Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Si-Jia Hong

    2012-05-01

    Full Text Available A pressurized liquid extraction (PLE and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS method was developed for the qualitative determination of saponins in different parts of <em>P. notoginsengem>, including rhizome, root, fibre root, seed, stem, leaf and flower. The samples were extracted using PLE. The analysis was achieved on a Zorbax SB-C18 column with gradient elution of acetonitrile and 8 mM aqueous ammonium acetate as mobile phase. The mass spectrometer was operated in the negative ion mode using the electrospray ionization, and a collision induced dissociation (CID experiment was also carried out to aid the identification of compounds. Forty one saponins were identified in different parts of <em>P. notoginsengem> according to the fragmentation patterns and literature reports, among them, 21 saponins were confirmed by comparing the retention time and ESI-MS data with those of standard compounds. The results showed that the chemical characteristics were obviously diverse in different parts of <em>P. notoginsengem>, which is helpful for pharmacological evaluation and quality control of <em>P. notoginseng.em>

  2. Mutation induction in mangosteen : Effect of mutagens on biochemical and histological changes.

    OpenAIRE

    Phrommee, V.; Te-chato S.

    1999-01-01

    Nodular calli induced from young red leaves raised in vitro were brought to irradiation with various doses of gamma rays and soaking in ethylmethanesulfonate (EMS) at various concentrations. Treated calli were examined histologically and biochemically in order to determine an optimum dose of both mutagens. Irradiation of calli twice with gamma rays at 40 grays caused more damage to epidermal cells than the other doses and 0.5% EMS. However, double irradiation followed by application of EMS at...

  3. URINARY MUTAGENICITY AS A BIOMARKER OF COOKED-MEAT-ASSOCIATED MUTAGENS AND RISK FOR COLORECTAL ADENOMA

    Science.gov (United States)

    Urinary Mutagenicity as a Biomarker of Cooked-Meat-Associated Mutagens and Risk for Colorectal AdenomaIn a controlled feeding study involving 60 subjects, we have investigated urinary mutagenicity as a biomarker of exposure to cooked-meat-associated mutagens. In a separa...

  4. Chemical Composition of <em>Artemisia annuaem> L. Leaves and Antioxidant Potential of Extracts as a Function of Extraction Solvents

    Directory of Open Access Journals (Sweden)

    Maznah Ismail

    2012-05-01

    Full Text Available This study was conducted to investigate the chemical and nutritional composition of <em>Artemisia annuaem> leaves in addition to determination of antioxidant potential of their extracts prepared in different solvents. Chemical composition was determined by quantifying fat, protein, carbohydrate, fiber, tocopherol, phytate, and tannin contents. Extraction of <em>A. annuaem> leaves, for antioxidant potential evaluation, was carried out using five solvents of different polarities, <em>i.e>., hexane, chloroform, ethyl acetate, methanol and water. Antioxidant potential was evaluated by estimating total phenolic (TPC, flavonoid (TFC contents, ferric reducing antioxidant power (FRAP, Trolox equivalent antioxidant capacity (TEAC, DPPH radical scavenging activity and lipid peroxidation. Efficiency of different solvents was compared for the yield of antioxidant extracts from leaf samples and a clear variation was observed. The highest TPC, TFC, TEAC, DPPH radical scavenging and lowest lipid peroxidation were observed in MeOH extracts, whereas aqueous extract exhibited high ferric reducing antioxidant power; suggesting MeOH to be the most favorable extractant.

  5. Influence of mutagens on enzymes of germinating seeds of cotton (Gossypium hirsutum L.)

    International Nuclear Information System (INIS)

    The activities of the enzymes amylases, protease and phosphatases were studied in cotton during germination. The seeds were treated with 100-500 Gy of gamma rays, 10-50 mM of EMS, CA and SA in two cultivated varieties viz.. MCU 5 and MCU 11. Activity pattern of amylases, protease and phosphatases in treated seeds were significantly altered from controls. The alteration were positively correlated with increasing dose/concentration of mutagens up to 300 Gy of gamma rays and 30 mM of EMS, CA and SA. The present study pave the ways to discuss the importance of the enzymes and mutagens in germination of cotton seeds. (author)

  6. Specificity in chemical profiles of workers, brood and mutualistic fungi in <em>Atta, Acromyrmexem>, and <em>Sericomyrmex> fungus-growing ants

    DEFF Research Database (Denmark)

    Richard, Freddie-Jeanne; Poulsen, Michael; Drijfhout, Falko; Jones, Graeme; Boomsma, Jacobus Jan

    2007-01-01

    , acetates, acids, and esters. The chemical profiles in all three species are likely to be sufficiently different to allow discrimination at the species and colony level and sufficiently similar within colonies to generate a relatively constant colony-specific chemical gestalt. The relative likelihood of...

  7. mutagenic treatments and selection under drought conditions in wheat

    International Nuclear Information System (INIS)

    this study was carried out in 2002/2003,2003/2004 and 2004/2005 seasons at the fields and laboratories of nuclear research center, inshas, egypt in an attempt to induce drought tolerant mutants in the egyptian bread wheat cultivars sids 1 and sakha 93 via gamma rays and EMS mutagens , variability parameters, heritability and genetic advance from selection in M2 and M3 bulks derived via these mutagens were estimated and yield superiority of the new induced mutants over parent cultivars and M2 bulks were evaluated under water-stress and non-stress conditions. significant differences were observed among M2 and among M3 bulks as well as among M3 families selected under water stress or non-stress for all studied traits. irradiation and EMS mutagens succeeded to induce new variability measured by increases in means, ranges, PCV and GVC parameters in many M2 and M3 bulks under stress and non-stress, comparing induced M2 with M3 bulks , for superiority in studied genetic parameters including heritability in board sense (hb2)and expected genetic advance (G A) from selection indicated that there were 3 common superior bulks in both M2 and M3 generations (Sd-RAD-1, Sd -RAD-3 and Sd -EMS-1) under non-stress, while under water stress there were two common superior bulks (Sd-EMS-3 and Sk-RAD-2).superiority of these induced bulks qualified them for predicting to achieve genetic improvement in grain yield via selection under drought stress and non-stress conditions

  8. The detection and analysis of mutagens: Final report, 1968--1986

    International Nuclear Information System (INIS)

    Our main objectives are: to develop and improve the Salmonella test for detecting environmental mutagens; to investigate the relationship between carcinogens and mutagens and to validate the Salmonella test for detecting carcinogens; to uncover significant unsuspected environmental mutagens/carcinogens; to investigate the theory of mutagenesis; to develop new methods for determining DNA damage by particular chemicals in individual people; to understand the role of oxygen radicals in DNA damage, cancer, and aging; and to investigate the role of anti-carcinogens in preventing DNA damage

  9. Avaliação da atividade mutagênica da infusão de Baccharis trimera (Less. DC. em teste de Allium cepa e teste de aberrações cromossômicas em linfócitos humanos Evaluation of mutagenic activity resulting from the infusion Baccharis trimera (Less. DC. using the Allium cepa test and a chromosomal test for aberrations in human lymphocytes

    Directory of Open Access Journals (Sweden)

    Daiane S. de Pinho

    2010-05-01

    Full Text Available A carqueja (Baccharis trimera(Less. DC. é uma planta medicinal da família Asteraceae muito utilizada como chá no sul do Brasil no tratamento de doenças renais, intestinas, estomacais e principalmente como emagrecedora. O objetivo desde trabalho foi de avaliar a mutagenicidade in vivoe in vitrodo chá e para isso foi realizado o teste de Allium cepaL. e o de aberrações cromossômicas em linfócitos humanos utilizando quatro tratamentos: T1 (água; T2 (20 g/L de carqueja; T3 (200 g/L de carqueja, e T4 (paracetamol, a 400 mg/L. Ambos os procedimentos foram analisados pelo teste Mann-Whitney U. Este estudo evidencia um efeito mutagênico do chá em células vegetais (Allium cepa e em células humanas (aberrações cromossômicas cultivadas, pois em ambos os testes, T2 e T3 obteve-se uma média mais elevada que nos outros tratamentos. Este estudo demonstra que o efeito é dependente da dose, portanto recomenda-se que o chá de carqueja seja consumido com moderação.Broom (Baccharis trimera(Less. DC. is a medicinal plant from Asteraceae that is commonly used as a tea in the south of Brazil for the treatment of renal, intestinal and stomach diseases. It is also used as a slimming agent. The aim of this study was to evaluate the mutagenicity of the tea in vivoand in vitro. In order to do this, the Allium cepatest was carried out and the chromosomal aberrations in human lymphocytes were tested using four treatments: T1 (water; T2 (20 g/L of broom; T3 (200 g/L of broom and T4 (paracetamol at 400 mg/L. Both procedures were analyzed using the Mann-Whitney U test. This study provided evidence of a mutagenic effect of the tea in vegetable cells (Allium cepa and in cultivated human cells. In tests T2 and T3 there was a higher average than the other treatments. This study shows that the effect is dependent on the dose. It is therefore recommended that broom tea be consumed with moderation.

  10. Analysis of Hexanitrostilbene (HNS) and Dipicryethane (DPE) for Mutagenicity by the Ames/Salmonella Assay

    Energy Technology Data Exchange (ETDEWEB)

    Wu, R; Felton, J

    2007-10-12

    The Ames/Salmonella assay, developed by Professor Bruce Ames at the University of California, Berkeley, is a rapid and sensitive assay for detecting mutagenicity of various chemical compounds (Maron and Ames, 1983). It is a widely accepted short-term assay for detecting chemicals that induce mutations in the histidine (his) gene of Salmonella typhimurium. This is a reverse mutation assay that detects the mutational reversion of his-dependent Salmonella to the his-independent counterpart. Thereby, mutagenic compounds will increase the frequency of occurrence of his-independent bacterial colonies. The assay utilizes the specific genetically constructed strains of bacteria either with or without mammalian metabolic activation enzymes (S9), Aroclor induced rat liver homogenate to assess the mutagenicity of different compounds. In this study, we will use the Ames/Salmonella assay to investigate the mutagenicity of Hexanitrostilbene (HNS) from both Bofors and Pantex, and Dipicryethane (DPE).

  11. Mutagenicity of some alkyl nitrites used as recreational drugs

    Energy Technology Data Exchange (ETDEWEB)

    Dunkel, V.C.; Cameron, T.P. (National Institute of Health, Bethesda (USA)); Rogers-Back, A.M.; Lawlor, T.E.; Harbell, J.W. (Microbiological Associates Inc., Rockville, MD (USA))

    1989-01-01

    When the AIDS epidemic was in its earliest stages, and prior to identification of HIV as the etiological factor, the use of volatile nitrites by the male homosexual community to enhance sexual activities appeared to have a significant role in this disease. Preliminary observations indicated that that portion of the male homosexual community which developed Kaposi's sarcoma were also heavy nitrite users. These nitrites had been demonstrated to be mutagenic in bacteria and thus it was postulated that they could be responsible for the appearance of the sarcoma. To evaluate further the genotoxic activity of these chemicals, six nitrites, including those most commonly used by homosexuals for sexual gratification, were selected for testing in the mouse lymphoma TK {plus minus} and Salmonell typhimurium mutagenicity assays. One chemical, n-amyl nitrite, was negative in the mouse lymphoma assay, while the other five chemicals, n-butyl, isobutyl, iso-amyl, sec-butyl, and n-propyl nitrite, were positive. All six compounds were positive in the Salmonella assay. The mutagenic and known toxic effects of these chemicals remain a concern because a large population of teenagers and young adults continue to abuse these substances.

  12. Mutation epidemiology and its prospects for detecting human germinal mutagens

    International Nuclear Information System (INIS)

    No germinal mutagen has been documented in man, with the possible exception of radiation. Nevertheless, results of studies in other species make it prudent and reasonable to believe that exposure of human germ cells to ionizing radiation and certain chemicals will cause mutations that will ultimately result in illness. The proliferation of test systems for mutagens in nonhuman species does not obviate but, in fact, presses the need for a demonstration of environmentally induced germinal mutation in human beings. Guidelines for protection from ionizing radiation in human beings have been largely extrapolated from observations in mice yet, the largest study of human populations exposed to a known mutagen of animals has, to date, shown that man may be more resistant than mice to genetic damage caused by the atomic bombs in Japan. The demonstration of what would seem an obvious biological conclusion - that what causes mutations in nonhuman species causes mutations in man - has been called ''one of the most difficult epidemiological issues ever faced by biomedical science''. Possible strategies have been considered repeatedly since the 1950s. At present, several large projects are under way to monitor certain manifestations of genetic damage, and formal protocols have been developed. Because the hazards of potential mutagens are world-wide and because it is difficult to gather sufficient number of exposed persons to detect significant changes in mutation rates, a WHO consultant group is developing protocols that, if accepted internationally, may provide answers

  13. Extending (Q)SARs to incorporate proprietary knowledge for regulatory purposes: A case study using aromatic amine mutagenicity.

    Science.gov (United States)

    Ahlberg, Ernst; Amberg, Alexander; Beilke, Lisa D; Bower, David; Cross, Kevin P; Custer, Laura; Ford, Kevin A; Van Gompel, Jacky; Harvey, James; Honma, Masamitsu; Jolly, Robert; Joossens, Elisabeth; Kemper, Raymond A; Kenyon, Michelle; Kruhlak, Naomi; Kuhnke, Lara; Leavitt, Penny; Naven, Russell; Neilan, Claire; Quigley, Donald P; Shuey, Dana; Spirkl, Hans-Peter; Stavitskaya, Lidiya; Teasdale, Andrew; White, Angela; Wichard, Joerg; Zwickl, Craig; Myatt, Glenn J

    2016-06-01

    Statistical-based and expert rule-based models built using public domain mutagenicity knowledge and data are routinely used for computational (Q)SAR assessments of pharmaceutical impurities in line with the approach recommended in the ICH M7 guideline. Knowledge from proprietary corporate mutagenicity databases could be used to increase the predictive performance for selected chemical classes as well as expand the applicability domain of these (Q)SAR models. This paper outlines a mechanism for sharing knowledge without the release of proprietary data. Primary aromatic amine mutagenicity was selected as a case study because this chemical class is often encountered in pharmaceutical impurity analysis and mutagenicity of aromatic amines is currently difficult to predict. As part of this analysis, a series of aromatic amine substructures were defined and the number of mutagenic and non-mutagenic examples for each chemical substructure calculated across a series of public and proprietary mutagenicity databases. This information was pooled across all sources to identify structural classes that activate or deactivate aromatic amine mutagenicity. This structure activity knowledge, in combination with newly released primary aromatic amine data, was incorporated into Leadscope's expert rule-based and statistical-based (Q)SAR models where increased predictive performance was demonstrated. PMID:26879463

  14. Quantitative mammalian cell mutagenesis and mutagen screening: study with CHO cells

    International Nuclear Information System (INIS)

    The CHO/HGPRT system has been developed and defined for quantifying mutation induced by various physical and chemical agents at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells. In all direct-acting chemical mutagens studied, mutation induction increases linearly as a function of the concentration, with no apparent threshold. Some chemicals induce mutation at non-cytotoxic concentrations. The mutagenicity of ethyl methanesulfonate has been quantified as a function of exposure concentration x treatment time. The sensitive and quantitative nature of the system enables studies of the structure-activity (mutagenicity) relationships of various classes of chemicals, including alkylating agents, heterocyclic nitrogen mustards, and platinum compounds. When rat liver S9-mediated metabolic activation is present, procarcinogens such as benzo(a)pyrene, 2-acetylaminofluorene, and dimethylnitrosamine are mutagenic, whereas their noncarcinogenic structural analogues pyrene, fluorene, and dimethylamine are not. The system has been shown to be useful in determining the interactive effects between physical and chemical agents, and in screening for mutagenicity of fractionated organic mixtures and industrial chemicals in both liquid and gaseous state. For the system to be used successfully in routine screening, further studies should be directed toward the development of a metabolic activation system suitable for a broad spectrum of chemicals, a sensitive and reliable statistical method, and an experimental design to determine compounds with low mutagenicity. The system has been expanded for determination of mutagen-induced chromosome aberration, sister-chromatid exchange, and micronucleus formation in addition to gene mutation and cytotoxicity; it can also be used to study inhibition of DNA synthesis

  15. Outcrossing in mutagenically treated plants

    International Nuclear Information System (INIS)

    Classification by sexual reproductive systems is arbitrary in that exclusive autogamy or allogamy seldom occurs. Various mechanisms can promote a certain mating system; the adherence is, however, dependent on environmental conditions at the time of flowering. The rate of cross-pollination in autogamous plant species is generally low and its practical significance for breeding procedures is negligible. Outcrossing in mutagen-treated material, on the other hand, is reported to increase by induced sterility and is especially influenced by male sterility mutations. Although increased cross-pollination can be observed in mutagenically treated autogamous plants, a change in the breeding procedure towards the methodology applied for allogamous crop plants does not seem to be warranted in procedures with practical and applied objectives. Some plant species can be classified as facultatively allogamous. Their degree of outcrossing depends largely on environmental conditions. Extreme values of 5 and 60% have been reported in the field bean (Vicia faba). While to our knowledge results on the effects of mutagen treatments on the mating pattern have not been published, prerequisites for changes towards allogamy by the aid of induced mutations are shortly discussed. (author)

  16. Combined anaerobic–ozonation process for treatment of textile wastewater: Removal of acute toxicity and mutagenicity

    Energy Technology Data Exchange (ETDEWEB)

    Punzi, Marisa, E-mail: marisa.punzi@biotek.lu.se [Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); Nilsson, Filip [Water and Environmental Engineering at the Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); Anbalagan, Anbarasan [Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); Svensson, Britt-Marie [School of Education and Environment, Kristianstad University, SE-291 88 Kristianstad (Sweden); Jönsson, Karin [Water and Environmental Engineering at the Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden); Mattiasson, Bo; Jonstrup, Maria [Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund (Sweden)

    2015-07-15

    Highlights: • COD and UV absorbance were effectively reduced. • The treated effluents were non-toxic to Artemia salina and Vibrio fischeri. • The real textile wastewater was mutagenic. • Mutagenicity persisted after bio treatment and even more after a short ozonation. • Higher ozone doses completely remove mutagenicity. - Abstract: A novel set up composed of an anaerobic biofilm reactor followed by ozonation was used for treatment of artificial and real textile effluents containing azo dyes. The biological treatment efficiently removed chemical oxygen demand and color. Ozonation further reduced the organic content of the effluents and was very important for the degradation of aromatic compounds, as shown by the reduction of UV absorbance. The acute toxicity toward Vibrio fischeri and the shrimp Artemia salina increased after the biological treatment. No toxicity was detected after ozonation with the exception of the synthetic effluent containing the highest concentration, 1 g/l, of the azo dye Remazol Red. Both untreated and biologically treated textile effluents were found to have mutagenic effects. The mutagenicity increased even further after 1 min of ozonation. No mutagenicity was however detected in the effluents subjected to longer exposure to ozone. The results of this study suggest that the use of ozonation as short post-treatment after a biological process can be beneficial for the degradation of recalcitrant compounds and the removal of toxicity of textile wastewater. However, monitoring of toxicity and especially mutagenicity is crucial and should always be used to assess the success of a treatment strategy.

  17. Application of the Ames Mutagenicity Test to Food Processed by Physical Preservation Methods

    International Nuclear Information System (INIS)

    An irradiated (380 krad) mixture of four fresh vegetables - leek, celery, carrot, and cauliflower - was examined for mutagenicity by the Ames mutagenicity test using four different histidine-deficient strains of Salmonellae. Water extracts were prepared from the irradiated and unirradiated vegetables - a freeze dried extract (FDE) and a boiled extract (BE). Several problems were overcome in the mutagenicity testing of a complex substance such as food which contains free histidine, different species of bacteria, and a mixture of low and high molecular weight chemicals. In addition, we eliminated an omission in the usual protocols of the Ames test by testing the positive mutagen controls in the presence and absence of the test samples, thus reducing the possible incidence of false negatives and false positives. The induction and expression of mutagenesis by sodium azide (SA) and ethidium bromide (EB) in TA 100 and TA 98 mutant strains, respectively, decreased with increasing amounts of FDE, while increasing levels of BE suppressed the number of revertants in TA 98 in the presence of EB, but exerted little influence on the mutagenicity of SA in TA 100. No difference was observed in the antimutagenic action between the irradiated and unirradiated vegetable extracts. Both the FDE and BE preparations suppressed the action of a frameshift mutagen, but with a base-pair mutagen only the FDE or uncooked vegetable extracts produced suppression. (author)

  18. Application of the Ames mutagenicity test to food processed by physical preservation methods

    International Nuclear Information System (INIS)

    An irradiated (380 krad) mixture of four fresh vegetables - leek, celery, carrot, and cauliflower - was examined for mutagenicity by the Ames mutagenicity test using four different histidine-deficient strains of Salmonellae. Water extracts were prepared from the irradiated and unirradiated vegetables - a freeze dried extract (FDE) and a boiled extract (BE). Several problems were overcome in the mutagenicity testing of a complex substance such as food which contains free histidine, different species of bacteria, and a mixture of low and high molecular weight chemicals. In addition, we eliminated an omission in the usual protocols of the Ames test by testing the positive mutagen controls in the presence and absence of the test samples, thus reducing the possible incidence of false negatives and false positives. The induction and expression of mutagenesis by sodium azide (SA) and ethidium bromide (EB) in TA 100 and TA 98 mutant strains, respectively, decreased with increasing amounts of FDE, while increasing levels of BE suppressed the number of revertants in TA 98 in the presence of EB, but exerted little influence on the mutagenicity of SA in TA 100. No difference was observed in the antimutagenic action between the irradiated and unirradiated vegetable extracts. Both the FDE and BE preparations suppressed the action of a frameshift mutagen, but with a base-pair mutagen only the FDE or uncooked vegetable extracts produced suppression. (author)

  19. Combined anaerobic–ozonation process for treatment of textile wastewater: Removal of acute toxicity and mutagenicity

    International Nuclear Information System (INIS)

    Highlights: • COD and UV absorbance were effectively reduced. • The treated effluents were non-toxic to Artemia salina and Vibrio fischeri. • The real textile wastewater was mutagenic. • Mutagenicity persisted after bio treatment and even more after a short ozonation. • Higher ozone doses completely remove mutagenicity. - Abstract: A novel set up composed of an anaerobic biofilm reactor followed by ozonation was used for treatment of artificial and real textile effluents containing azo dyes. The biological treatment efficiently removed chemical oxygen demand and color. Ozonation further reduced the organic content of the effluents and was very important for the degradation of aromatic compounds, as shown by the reduction of UV absorbance. The acute toxicity toward Vibrio fischeri and the shrimp Artemia salina increased after the biological treatment. No toxicity was detected after ozonation with the exception of the synthetic effluent containing the highest concentration, 1 g/l, of the azo dye Remazol Red. Both untreated and biologically treated textile effluents were found to have mutagenic effects. The mutagenicity increased even further after 1 min of ozonation. No mutagenicity was however detected in the effluents subjected to longer exposure to ozone. The results of this study suggest that the use of ozonation as short post-treatment after a biological process can be beneficial for the degradation of recalcitrant compounds and the removal of toxicity of textile wastewater. However, monitoring of toxicity and especially mutagenicity is crucial and should always be used to assess the success of a treatment strategy

  20. Mutagenic activity of disinfection by-products.

    OpenAIRE

    Cognet, L; Courtois, Y; Mallevialle, J

    1986-01-01

    Data on raw water quality, disinfection treatment practices, and the resulting mutagenic properties of the treated water were compiled from pilot- and full-scale treatment experiments to evaluate that parameter which might produce variability in the results of a mutagenic study. Analysis of the data and comparison of treatment practices indicated that the measured mutagenic activity is strongly related to the characteristics of the organic matter in the raw water, the methodology used to samp...

  1. Human somatic, germinal and heritable mutagenicity

    International Nuclear Information System (INIS)

    This report deals with the general process of variant formation rather than with the consequences of a specific variant being present. It focusses on mutational mechanisms, mutagens, and the method for detecting de novo mutants and estimating mutation rate. It is to human genetics much like disease causation and prevention medicine are to medicine as a whole. The word ''mutagenicity'' is used in the title and throughout the text to connote the causation of all classes of genetic damage. Mutagenicity and the corresponding words mutation, mutagen and mutagenesis can have multiple meaning, sometimes relating to gene mutation, sometimes to heritable mutation, and somtimes to all types of genetic damage. 38 refs., 1 tab

  2. Assessment of the mutagenic potential of Skadar Lake sediments using the salmonella microsomal assay

    Directory of Open Access Journals (Sweden)

    Perović Svetlana

    2013-01-01

    Full Text Available Organic extracts of sediments from Skadar Lake (National park and protected RAMSAR site were investigated for their mutagenic potential using Salmonella typhimurium TA98 and TA100 strains in the presence and absence of metabolic activation. Five different concentrations of sediment extracts from five different sampling sites were assayed, and mutagenic results were obtained for both strains. These studies present a part of the EULIMNOS project and included a battery of bioassay testing and in situ investigations of microbial community structures of Skadar Lake sediments. The obtained results show mutagenic risk potential in five collected samples in the absence and presence of metabolic activation. Additional studies are required in order to identify and quantify the chemical compounds responsible for the mutagenic activity present in Skadar Lake sediments.

  3. Using of simultaneous phytotoxicity and mutagenicity test in assessing the risk of waste from landfill of gas generator Kalinovo

    International Nuclear Information System (INIS)

    Storage of waste can have a negative impact on the environment by its chemical composition. During mutual chemical reactions a variety of hazardous compounds originates that seriously threaten human health. With increasing requirements to human health and environmental protection plant tests were introduced as very important and inexpensive indicators of mutagenicity and phytotoxicity. In this paper we present application of simultaneous test of mutagenicity and phytotoxicity on selected indicator plant species in conditions 'in situ'. Based on the observed results we conclude that the waste from generator gas in the repository Kalinovo has high toxicity and mutagenicity. (authors)

  4. Comparative study of the effect of chemical and physical factors on the variability of almond

    International Nuclear Information System (INIS)

    Six vareties of almond: Texas, Krymskij, Nek-ultra, Logindok, Drejk and Myagkoskorlupyj have been studied. It is shown that gamma-irradiation at a dose up to 16 kr induces 16 to 43 % changes in plants. A dose of 32 kr induces 33 to 60 % changes, 40 t0 50 kr - 65 to 90 % changes, but with a less percentage of survived plants. High doses of gamma-radiation induces a broad spectrum of variability, is not observed under the treatment of seeds with chemical mutagens. A dose of 32 to 40 kr has been found to be critical for the varieties studied, a dose of 50 kr - to be lethal. At a dose of 50 kr the seedlings either do not survive to the end of the vegetative period, or only 3 to 5 plants survie. A chemical mutagen, ethylmethanesulphonate (EMS) at a concentration of 0.1 to 0.2 % produc 12 to 13 % morphological changes, the percentage of survived plants being higher as compared with gamma-radiation. The mutagen concentration of 0.4% produces 90 to 93 % changed plants, the plant survival being 50 to 57 %. The 0.4% concentration can be taken as a critical one for the varieties studied. Comparative evaluations of gamma-radiation and chemical mutagens reveale that EMS at concentrations studied produces 3 to 4 times more changes than gamma-rays, the percentage of survived plants being also 3 to 4 times higher. However, EMS produces monotypic changes, while gamma-radiation induces a broad spectrum of variability. Thus, EMS can be used as a factor inducing dwarf varieties of almond and other fruit cultures

  5. 50. Mutagenicity Study of Meloxicam

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@The Meloxicam is a drug for diminishing inflammation and contraining ache, and has good curative effect on clinic. In order to ascertain the safety of it, mutagenicity of Meloxicam was studied by using Ames test、 chromosome test and micronuclus test. ①Ames test: Meloxicam at six concentrations of 5 000、2 000、200、20、2、0.2 μg/plate were studied by using TA97、TA98、TA100、TA102 bacterial plant

  6. ASSESMENT OF BIOCHEMICAL ATTRIBUTES OF PRAECITRULLUS FISTULOSUS TREATED WITH MUTAGENS

    Directory of Open Access Journals (Sweden)

    Mehreen Khan

    2016-02-01

    Full Text Available Plants are well known to have certain primary and secondary metabolites collectively are known as biochemicals that plays an important role for human health as their medicinal properties. The aim of present study was to enhance and evaluate biochemical profile of Praecitrullus fistulosus by induced mutagenesis to cause genetic variations, plant leaves were treated with different chemical and physical mutagens. Colchicine and Ethidium bromide were used as chemical mutagens. While Ultraviolet (UV rays and X- rays were used as physical mutagens for the treatment of seeds. After the eleventh week of their growth, methanol extracts of dried leaves were prepared and further analyzed for the estimation of biochemicals. It was observed that total carbohydrates, total Proteins, phenolic compounds, antioxidant activity, reducing power, ascorbic Acid  and Chlorophyll a, were found significantly (p<0.05 higher in Colchicine 0.02% treated plants, while reducing sugars were significantly (p<0.05  increases in Colchicine 0.01% treated plants as compared to control plants. Total flavonoids, total flavonol, Chlorophyll b and Carotenoids were increases significantly (p<0.05 in plants treated with 0.05% Ethidium bromide while tannin content was increased significantly (p<0.05 in 0.10% Ethidium bromide treated plants as compared to the  control plants.

  7. Genotoxic and mutagenic effects of sewage sludge on higher plants.

    Science.gov (United States)

    Corrêa Martins, Maria Nilza; de Souza, Victor Ventura; Souza, Tatiana da Silva

    2016-02-01

    Sewage treatment yields sludge, which is often used as a soil amendment in agriculture and crop production. Although the sludge contains elevated concentrations of macro and micronutrients, high levels of inorganic and organic compounds with genotoxic and mutagenic properties are present in sludge. Application of sludge in agriculture is a pathway for direct contact of crops to toxic chemicals. The objective of this study was to compile information related to the genotoxic and mutagenic effects of sewage sludge in different plant species. In addition, data are presented on toxicological effects in animals fed with plants grown in soils supplemented with sewage sludge. Despite the benefits of using sewage sludge as organic fertilizer, the data showcased in this review suggest that this residue can induce genetic damage in plants. This review alerts potential risks to health outcomes after the intake of food cultivated in sewage sludge-amended soils. PMID:26643763

  8. Chemical Carcinogenesis Research Information System (CCRIS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The CCRIS database contains chemical records with carcinogenicity, mutagenicity, tumor promotion, and tumor inhibition test results. CCRIS provides historical...

  9. Intervarietal differences in response of sunflower (Helianthus annuus L.) to different mutagenic treatments

    International Nuclear Information System (INIS)

    For much of the past century, mutagenesis has gained popularity in plant genetics research as a means of inducing novel genetic variation. Induced mutations have been applied for the past 40 years to produce mutant cultivars in sunflower by changing plant characteristics that significantly increase plant yield and quality. The present study was focused on generating baseline data to elucidate the role of genotypic differences in the response of sunflower to induced mutagenesis with the aim of expanding the applicability of the use of induced mutant stocks in the genetic improvement of the crop and in its functional genomics. The strategy adopted was to estimate the optimal treatment conditions (doses of mutagens) through relating the extent of damage in seedling progeny to the exposure levels of the initiating propagules to mutagens. Seeds of fifteen elite sunflower genotypes of commonly used as breeding stocks and grown on commercial scales were treated with a range of mutagens: gamma rays (γ rays); fast neutrons and with ethyle-methane-sulphonate (EMS) at different treatment doses. The three mutagenic agents affected seedling height, reducing it with increasing dosage. Based on the mutagen damage on seedling height, the 50% and 30% damage indices (D50 and D30, respectively) were estimated for the 15 sunflower genotypes for the three mutagens. The D50 (D30) values for the sunflower lines ranged from 120 to 325Gy (5 to 207Gy) for gamma irradiation; 9 to 21Gy (0.1 to 10Gy) for fast neutrons and 0.69 to 1.55% (0.01 to 0.68%) concentration of EMS. (author)

  10. Intervarietal Differences in Response of Sunflower (Helianthus annuus L.) to Different Mutagenic Treatments

    International Nuclear Information System (INIS)

    For much of the past century, mutagenesis has gained popularity in plant genetics research as a means of inducing novel genetic variation. Induced mutations have been applied for the past 40 years to produce mutant cultivars in sunflower by changing plant characteristics that significantly increase plant yield and quality. The present study was focused on generating baseline data to elucidate the role of genotypic differences in the response of sunflower to induced mutagenesis with the aim of expanding the applicability of the use of induced mutant stocks in the genetic improvement of the crop and in its functional genomics. The strategy adopted was to estimate the optimal treatment conditions (doses of mutagens) through relating the extent of damage in seedling progeny to the exposure levels of the initiating propagules to mutagens. Seeds of 15 elite sunflower genotypes commonly used as breeding stocks and grown on commercial scales were treated with a range of mutagens: Gamma-rays (γ rays); fast neutrons and with ethyle-methane-sulphonate (EMS) at different treatment doses. The three mutagenic agents affected seedling height, reducing it with increasing dosage. Based on the mutagen damage on seedling height, the 50% and 30% damage indices (D50 and D30, respectively) were estimated for the 15 sunflower genotypes for the three mutagens. The D50 (D30) values for the sunflower lines ranged from 120 to 325Gy (5 to 207Gy) for gamma irradiation; 9 to 21Gy (0.1 to 10Gy) for fast neutrons and 0.69 to 1.55% (0.01 to 0.68%) concentration of EMS. (author)

  11. Modulation of the mutagenic effect of benzo[a]pyrene and bleomycin by isoflavone extracts in a rat hepatoma cell line Modulação do efeito mutagênico do benzo[a]pireno e bleomicina por extratos de isoflavonas em células de hepatoma de roedor

    Directory of Open Access Journals (Sweden)

    Mário Sérgio Mantovani

    2012-06-01

    Full Text Available Epidemiologic studies show that the intake of foods rich in isoflavones (phytoestrogens, such as soybeans, confers protection against various types of cancer, what increases the scientific and popular interest on these compounds. In the present study, phytoestrogens extracts from soybeans were tested for genotoxic potential and modulatory effects on benzo[a]pyrene and bleomycin. Two phytoestrogens were evaluated in vitro, phytoestrogen “A” was supplied by EMBRAPA-Soja, Londrina – PR, and phytoestrogen “B” was purchased in a local drug store. The methods used were the comet assay (genotoxicity and antigenotoxicity and micronucleus test with cytokinesis block (mutagenicity in rat hepatoma cells (HTC cell. The isoflavones were tested at three concentrations pre-established by the MTT cytotoxicity assay. Both isoflavone extracts showed no genotoxic effects in the comet assay, but showed induction of micronucleus. In the evaluation of the phytoestrogens for a modulatory effect, both phytoestrogens extracts showed antigenotoxicity in the comet assay.Estudos epidemiológicos mostram que a ingestão de alimentos ricos em isoflavonas (fitoestrógenos, como a soja, confere proteção contra vários tipos de câncer, o que aumenta o interesse científico e popular sobre esses compostos. No presente estudo, os fitoestrógenos de extrato de soja foram testados quanto aos efeitos genotóxicos e modulador de benzo [a] pireno e bleomicina. Dois fitoestrogênios foram avaliados in vitro, o fitoestrógenos “A” foi fornecido pela Embrapa-Soja, Londrina - PR, e o fitoestrógenos “B” foi comprado em uma farmácia de manipulação local. Os métodos utilizados foram o teste do Cometa (genotoxicidade e antigenotoxicidade e teste do Micronúcleo com Bloqueio Citocinese (mutagenicidade em células de hepatoma de rato (HTC celulares. As isoflavonas foram testadas em três concentrações pré-estabelecidas pelo ensaio de citotoxidade MTT. Ambos os

  12. Investigating the Mutagenic Effects of Three Commonly Used Pulpotomy Agents Using the Ames Test

    Directory of Open Access Journals (Sweden)

    Mohammad Samiei

    2015-03-01

    Full Text Available Purpose: The mutagenic potency of materials used in dentistry is of great concern. The Ames test is a bacterial reverse mutation assay, which is used to determine the mutagenicity potential of chemicals. In this study, the Ames test was used to compare mutagenic effects of three pulpotomy agents, namely, CEM cement, formocresol and ferric sulfate. Methods: TA100 strain of Salmonella typhimurium was used to evaluate mutagenicity of different concentrations of pulpotomy materials in the presence and absence of enzymatic system found in rat liver S9 fraction. Negative controls were 1% dimethyl sulfoxide and water. The positive controls were sodium azide and 2-aminoanthracene. The number of colonies per plate was counted. The material was regarded mutagenic if the number of histidine revertant colonies was twice or more than the spontaneous revertant colonies (Ames mutagenicity ratio. Results: Ferric sulfate was found mutagenic in the concentrations prepared by addition of 50 μL of its 1 in 100 and 1 in 1000 times diluted solutions to the culture medium in the absence of S9 fraction (Ames test ratios of 2.8 and 2.2, respectively. Formocresol showed strong toxicity toward TA100 strain of S. typhimurium up to the concentration as low achieved using 1000 times diluted solution of the original preparation, particularly in the presence of S9 fraction. Ames assay failed to detect significant reverse mutations in all the concentrations of CEM cement. Conclusion: In contrast to formocresol and ferric sulfate, CEM cement is a less toxic and non-mutagenic agent.

  13. Investigating the Mutagenic Effects of Three Commonly Used Pulpotomy Agents Using the Ames Test

    Science.gov (United States)

    Samiei, Mohammad; Asgary, Saeed; Farajzadeh, Malak; Bargahi, Nasrin; Abdolrahimi, Majid; Kananizadeh, Usef; Dastmalchi, Siavoush

    2015-01-01

    Purpose: The mutagenic potency of materials used in dentistry is of great concern. The Ames test is a bacterial reverse mutation assay, which is used to determine the mutagenicity potential of chemicals. In this study, the Ames test was used to compare mutagenic effects of three pulpotomy agents, namely, CEM cement, formocresol and ferric sulfate. Methods: TA100 strain of Salmonella typhimurium was used to evaluate mutagenicity of different concentrations of pulpotomy materials in the presence and absence of enzymatic system found in rat liver S9 fraction. Negative controls were 1% dimethyl sulfoxide and water. The positive controls were sodium azide and 2-aminoanthracene. The number of colonies per plate was counted. The material was regarded mutagenic if the number of histidine revertant colonies was twice or more than the spontaneous revertant colonies (Ames mutagenicity ratio). Results: Ferric sulfate was found mutagenic in the concentrations prepared by addition of 50 µL of its 1 in 100 and 1 in 1000 times diluted solutions to the culture medium in the absence of S9 fraction (Ames test ratios of 2.8 and 2.2, respectively). Formocresol showed strong toxicity toward TA100 strain of S. typhimurium up to the concentration as low achieved using 1000 times diluted solution of the original preparation, particularly in the presence of S9 fraction. Ames assay failed to detect significant reverse mutations in all the concentrations of CEM cement. Conclusion: In contrast to formocresol and ferric sulfate, CEM cement is a less toxic and non-mutagenic agent. PMID:25789229

  14. Effect of mutagens on the quality characters and disease resistant genes of diploid cotton (Gossypium arboreum L.)

    International Nuclear Information System (INIS)

    In both M1 and M2 plant height decreased with the increase in dose for both the mutagens. The 15 Krad and 0.15M EMS doses increased 122.7 and 128.3 gm seed cotton yield as compared to control respectively while all other doses of both mutagens decreased the yield of seed cotton. The EMS dose 0.10 M drastically decreased 184 gm seed cotton yield as compared to control. There was no larger effect of both mutagens on GOT % whereas staple length was slightly increased and micronaire value decreased as compared to control for all the doses of both mutagens. It was observed in M2 that mutation dose 10 Krad increased 165.6 gm seed cotton yield as compared to control but slight reduction in GOT % was observed. In M2 GOT were increased 3.5 % with 15 Krad and 3.6 % with EMS 0.10 M as compared to control. There were no larger effects for both mutagens in case of staple length, micronaire and uniformity ratio for all the doses as compared to control. respectively. In both M1 and M2 no plant was observed susceptible to cotton leaf curl virus and bacterial blight diseases of cotton

  15. Studies on Potential Mutagenic and Genotoxic Activity of Setarud

    Directory of Open Access Journals (Sweden)

    B Farzamfar

    2008-09-01

    Full Text Available Background: Setarud (IMODTM is a new herbal drug that has demonstrated immune modulating activity in preliminary investigations. The aim of this study was to evaluate the potential of mutagenicity and genotoxic properties of Setarud following the guidelines of the Organization for Economic Co-operation and Development (OECD for the Testing of Chemicals. Methods: Ames Salmonella/mammalian microsome mutagenesis assay was used to evaluate the ability of the drug and its metabolites to induce mutation in Salmonella tester strains. Setarud was applied in concentrations of 0.1-1000 µg/dish. The effect of the drug metabolites which were formed in the presence of rat liver microsomal fraction S9 was investigated using complete and incomplete microsomal activation mixtures, separately. Induction of dominant lethal mutations in spermatogenic stem cells of male mice was also assessed. Results: In the Ames test, the drug preparation did not cause a significant increase in the number of revertant bacterial colonies as compared with negative control meaning that Setarud within the tested range did not exhibit mutagenic activity. The level of post-implantation losses and as a result the number of lethal mutations in germ cells at different stages of spermatogenesis in mice treated with Setarud was not statistically higher than that of control. Conclusion: Under experimental conditions which were employed, the drug was not mutagenic or genotoxic.

  16. Alkaline azide mutagenicity in cowpea

    International Nuclear Information System (INIS)

    Sodium azide is known as a potent mutagen in cereals and legumes. It is very effective in acidic medium in barley. Here an attempt is made to measure the effectiveness of sodium azide in alkaline medium (pH 7.4) on cowpea (Vigna unguiculata (L.) Walp., variety FS-68). Seeds pre-soaked in distilled water for 5 hours were treated with different concentrations (10-6, 10-5, 10-4 and 10-3M) of sodium azide (NaN3) for 4 hours at 28± 2 deg. C. Bottles were intermittently shaken, then the seeds were thoroughly washed in running tap water and subsequently planted in pots. The treatment caused significant biological damage such as reduction in seed germination, length of root and shoot, number of nodules and pods per plant and morphological leaf variations. Morphological, as well as chlorophyll mutants, were detected in M2

  17. The evolution of plant chemical defence - new roles for hydroxynitrile glucosides in <em>Lotus japonicusem>

    DEFF Research Database (Denmark)

    Knudsen, Camilla

    function and evolution. Further, it contributes to our understanding of the formation and role of biosynthetic gene clusters in plant chemical defence. The bifurcation in hydroxynitrile glucoside biosynthesis and catabolism observed in Lotus japonicus makes it a very suitable model system to study the......Plants are sessile organisms well-known to produce a vast array of chemical compounds of which many are used in chemical defence against herbivores and pathogens. The biosynthesis of these plant chemical defence compounds poses a considerable risk of self-toxicity for the plant itself. Several...... types of adaptations enable plants to avoid the potential lethal effects of their own defence compounds. These adaptations include detoxification and stabilization by glycosylation and the genomic clustering of biosynthetic pathway genes. These two types are the main focus of this PhD thesis on...

  18. Avaliação da mutagênese e genotoxicidade em Eigenmannia virescens (Teleostei: Gymnotiformes) expostos ao benzeno Avaliation of mutagenicity and gentotoxicity in Eigenmannia virescens (Teleostei: Gymnotiformes) exposed to benzene

    OpenAIRE

    Augusto Bücker; Wanderson Carvalho; José Antonio Alves-Gomes

    2006-01-01

    Os efeitos de substâncias genotóxicas sobre o genoma de peixes tem sido objeto de muitos estudos, sobretudo daqueles que buscam estabelecer a resposta dos genes aos estímulos ambientais. O presente trabalho teve como objetivo realizar um estudo sobre mutagenicidade e genotoxicidade em peixes elétricos da espécie Eingenmannia virescens, pela exposição ao benzeno (50ppm), utilizando as técnicas da Freqüência de Micronúcleos (MNs) e o Ensaio do Cometa. Foram coletadas amostras do sangue de dez p...

  19. Mutagenic and carcinogenic properties of drinking water

    International Nuclear Information System (INIS)

    In this chapter results of oxidation treatments with chlorine, ozone, chlorine dioxide, and ultraviolet (UV), with respect to their effects on activity (Ames test) in drinking water supplies are reviewed. In addition, the authors present the preliminary results of a pilot plant study on the effects of chlorine and chlorine dioxide on mutagenicity. Furthermore, results of several carcinogenicity studies performed with organic drinking water concentrates are discussed in relation to the results of a Dutch carcinogenicity study with mutagenic drinking water concentrates

  20. Mutagens affect food and water biodeteriorating fungi

    OpenAIRE

    Paterson, R. R. M.; Lima, Nelson

    2015-01-01

    Many areas of food mycology could be affected detrimentally by mutation of wild type fungi. Some of these will contact mutagens from pre-isolation to experimentation and the effect on fungi isolated from mycotoxin-contaminated food is assessed for the first time in this review. However, this mutagen issue is not considered by other authors in primary research papers, which is relevant to molecular biology techniques for gene sequencing, phylogenetics, diagnostics and myco...

  1. Mutagenicity in Salmonella typhimurium of some angelicin derivates proposed as new monofunctional agents for the photochemotherapy of psoriasis

    International Nuclear Information System (INIS)

    A series of angelicin in derivatives were testet for their mutagenic activity with and without near-ultraviolet irradiation (NUV) in Salmonella typhimurium strains. After irradiation with NUV, the tested compounds induced different numbers of revertants in strain TA100, indicating that the mutational events involved are base substitutions. In the dark, 3 chemicals behaved as frame-shift mutagens causing reversion in strain TA98. (orig.)

  2. Hyper production of alkaline protease by mutagenized bacillus subtilis

    International Nuclear Information System (INIS)

    The purpose of this work was to augment the alkaline protease production from Bacillus subtilis by using chemical mutagen (MMS) and UV mutagenesis. A number of mutants were isolated which produce high levels of extra cellular proteases. Analysis of culture supernatants of these mutants had shown that the total amounts of proteolysis activity were increased from 1 to 2 fold over the wild strain. Clones showing promote response were further characterized by analyzing different parameters; like of Temperature, pH substrate concentration and incubation period, to study the activity of protease enzyme. (author)

  3. Evolutionary Ensemble for In Silico Prediction of Ames Test Mutagenicity

    Science.gov (United States)

    Chen, Huanhuan; Yao, Xin

    Driven by new regulations and animal welfare, the need to develop in silico models has increased recently as alternative approaches to safety assessment of chemicals without animal testing. This paper describes a novel machine learning ensemble approach to building an in silico model for the prediction of the Ames test mutagenicity, one of a battery of the most commonly used experimental in vitro and in vivo genotoxicity tests for safety evaluation of chemicals. Evolutionary random neural ensemble with negative correlation learning (ERNE) [1] was developed based on neural networks and evolutionary algorithms. ERNE combines the method of bootstrap sampling on training data with the method of random subspace feature selection to ensure diversity in creating individuals within an initial ensemble. Furthermore, while evolving individuals within the ensemble, it makes use of the negative correlation learning, enabling individual NNs to be trained as accurate as possible while still manage to maintain them as diverse as possible. Therefore, the resulting individuals in the final ensemble are capable of cooperating collectively to achieve better generalization of prediction. The empirical experiment suggest that ERNE is an effective ensemble approach for predicting the Ames test mutagenicity of chemicals.

  4. Linking mutagenic activity to micropollutant concentrations in wastewater samples by partial least square regression and subsequent identification of variables.

    Science.gov (United States)

    Hug, Christine; Sievers, Moritz; Ottermanns, Richard; Hollert, Henner; Brack, Werner; Krauss, Martin

    2015-11-01

    We deployed multivariate regression to identify compounds co-varying with the mutagenic activity of complex environmental samples. Wastewater treatment plant (WWTP) effluents with a large share of industrial input of different sampling dates were evaluated for mutagenic activity by the Ames Fluctuation Test and chemically characterized by a screening for suspected pro-mutagens and non-targeted software-based peak detection in full scan data. Areas of automatically detected peaks were used as predictor matrix for partial least squares projections to latent structures (PLS) in combination with measured mutagenic activity. Detected peaks were successively reduced by the exclusion of all peaks with lowest variable importance until the best model (high R(2) and Q(2)) was reached. Peaks in the best model co-varying with the observed mutagenicity showed increased chlorine, bromine, sulfur, and nitrogen abundance compared to original peak set indicating a preferential selection of anthropogenic compounds. The PLS regression revealed four tentatively identified compounds, newly identified 4-(dimethylamino)-pyridine, and three known micropollutants present in domestic wastewater as co-varying with the mutagenic activity. Co-variance between compounds stemming from industrial wastewater and mutagenic activity supported the application of "virtual" EDA as a statistical tool to separate toxicologically relevant from less relevant compounds. PMID:26070082

  5. Carcinogens, Teratogens and Mutagens: Their Impact on Occupational Health, Particularly for Women in Veterinary Medicine.

    Science.gov (United States)

    Milligan, J. E.; And Others

    1983-01-01

    Pregnant women, especially those working in veterinary medicine, face occupational health/disease risks from mutagens, teratogens, and carcinogens. These hazards can be placed into three categories: physical, chemical, and biological. Each of these hazards is discussed with examples. (Author/JN)

  6. COMPARATIVE MUTAGENICITY OF HALOGENATED PYRIDINES IN THE 'SALMONELLA TYPHIMURIUM'/MAMMALIAN MICROSOME TEST

    Science.gov (United States)

    The Salmonella/microsome assay with strains TA97, TA98, TA100, and TA102 was used to examine the potential mutagenicity and structure-activity of 16 mono- and dihalogenated pyridines. The chemical reactivity of the halopyridines suggests that nucleophilic displacement of halogens...

  7. AZO DYES ARE MAJOR CONTRIBUTORS TO THE MUTAGENIC ACTIVITY DETECTED IN THE CRISTAIS RIVER WATERS

    Science.gov (United States)

    To determine if compounds from a dye processing plant were contributing to the mutagenicity repeatedly found in the Cristais River, Sao Paulo, Brazil, we chemically characterized the treated industrial effluent, raw and treated water, and the sludge produced by a Drinking Water T...

  8. THE CONTRIBUTION OF AZO DYES TO THE MUTAGENIC ACTIVITY OF THE CRISTAIS RIVER

    Science.gov (United States)

    To verify if compounds within the discharge of a dye processing plant were contributing to the mutagenicity repeatedly found in the Cristais River, Sao Paulo, Brazil, we chemically characterized the treated industrial effluent, raw and treated water, and the sludge produced by a ...

  9. Controle Químico de Antracnose em Mudas de Pupunheira em Viveiro Chemical Control of Anthracnose on Peach Palm Transplants in Orchard

    OpenAIRE

    Rudimar Mafacioli; Dauri José Tessmann; Álvaro Figueredo dos Santos; João Batista Vida

    2011-01-01

    A antracnose, causada pelo fungo <em>Colletotrichum loeosporioides>, é a principal doença da parte aérea da pupunheira (<em>Bactris gasipaes em>var gasipaes) em viveiros de mudas no Centro-Sul do Brasil. O objetivo do trabalho foi avaliar a eficiência de novas formulações de fungicidas no controle de antracnose em mudas de pupunheira, na fase de viveiro. O ensaio foi conduzido no período de abril a agosto de 2006, com o delineamento experimental em blo...

  10. Chemical signals and their interactions change transpiration processes in tomato wild-type and <em>flacca em>mutant

    DEFF Research Database (Denmark)

    Prokic, Ljiljana; Wollenweber, Bernd; Stikic, Radmila

    2011-01-01

    effects of chemicalsignals on the mechanism of transpiration of isolated leaves of L. esculentum Mill. cv. Ailsa Craig (WT) and mutant flacca. In bioassays, exogenic activity of different ABA concentrations and pH were tested in both genotype of tomato in order to stimulate chemical signals occurring...... drought conditions xylem sap pH was higher in leaves extract than in extracts of roots, indicating that an apoplastic pH gradient exists in WT, but not in flacca plants. So, reactions of flacca could not be only the result of lower ABA concentration, but also the result of an altered sensitivity to...

  11. The OSIRIS Weight of Evidence approach: ITS mutagenicity and ITS carcinogenicity.

    Science.gov (United States)

    Buist, Harrie; Aldenberg, Tom; Batke, Monika; Escher, Sylvia; Klein Entink, Rinke; Kühne, Ralph; Marquart, Hans; Pauné, Eduard; Rorije, Emiel; Schüürmann, Gerrit; Kroese, Dinant

    2013-11-01

    Risk assessment of chemicals usually implies data evaluation of in vivo tests in rodents to conclude on their hazards. The FP7 European project OSIRIS has developed integrated testing strategies (ITS) for relevant toxicological endpoints to avoid unnecessary animal testing and thus to reduce time and costs. This paper describes the implementation of ITS mutagenicity and carcinogenicity in the public OSIRIS webtool. The data requirements of REACH formed the basis for these ITS. The main goal was to implement procedures to reach a conclusion on the adequacy and validity of available data. For the mutagenicity ITS a quantitative Weight of Evidence approach based on Bayesian statistics was developed and implemented. The approach allows an overall quality assessment of all available data for the five types of mutagenicity data requirements: in vitro bacterial mutagenicity, in vitro and in vivo chromosome aberration, in vitro and in vivo mammalian mutagenicity. For the carcinogenicity ITS a tool was developed to evaluate the quality of studies not conforming (entirely) to guidelines. In a tiered approach three quality aspects are assessed: documentation (reliability), study design (adequacy) and scope of examination (validity). The quality assessment is based on expert and data driven quantitative Weight of Evidence. PMID:23357514

  12. Mutagenicity of eluent by hot water extraction of various coals: Effect of chlorination

    Energy Technology Data Exchange (ETDEWEB)

    Tsunenori Nakajima; Hiroyuki Hasegawa; Satomi Nakamata; Hirokazu Takanashi; Akira Ohki [Kagoshima University, Kagoshima (Japan). Department of Bioengineering

    2008-10-15

    Six kinds of powdery coals (two bituminous coals, two sub-bituminous coals, and two lignites) were extracted by hot water, and the eluents obtained were analyzed for total organic carbon (TOC), absorbance at 260 nm (A260), and pH. The TOC in the eluents decreased in the order, lignites > sub-bituminous coals > bituminous coals. The eluents of lignite gave high A{sub 260}/TOC values and fairly low pH compared to other coals. Chemical structure of the organic matter eluted from coals was discussed with the aid of FTIR analysis. The coal eluents were analyzed by the Ames mutagenicity assay using Salmonella typhimurium TA100 and TA98 strains, and no mutagenicity was observed for all of the six coals. However, especially for the lignites, chlorination of the eluents produced an appreciable mutagenicity, and the expression of mutagenicity was dependent upon the type of coal. The mutagenicity was extinguished when metabolic activation (rat liver homogenate, +S9) was applied. 26 refs., 6 tabs.

  13. Light-induced mutagenicity in Salmonella TA102 and genotoxicity/cytotoxicity in human T-cells by 3,3'-dichlorobenzidine: a chemical used in the manufacture of dyes and pigments and in tattoo inks

    International Nuclear Information System (INIS)

    DCB, 3,3'-dichlorobenzidine, is used primarily as an intermediate in the manufacture of diarylide yellow or azo red pigments for printing ink, textile, paint, and plastics. It is also used in tattoo inks. In this article, we investigate light-induced toxicity of DCB in both bacteria and human Jurkat T-cells. DCB itself is not toxic or mutagenic to Salmonella typhimurium TA102, but is photomutagenic at concentrations as low as 2 μM and phototoxic at concentrations >100 μM when bacteria are exposed to DCB and light at the same time (1.2 J/cm2 of UVA and 2.1 J/cm2 of visible light). Furthermore, DCB is both photocytotoxic and photogenotoxic to human Jurkat T-cells. Under a light irradiation dose of 2.3 J/cm2 of UVA and 4.2 J/cm2 of visible light, it causes the Jurkat T-cells to become nonviable in a DCB dose-dependent manner and the nonviable cells reaches 60% at DCB concentrations higher than 50 μM. At the same time, DNA fragmentation is observed for cells exposed to both DCB and light, determined by single cell gel electrophoresis (alkaline comet assay). As much as 5% (average) DNA fragmentation was observed when exposed to 200 μM DCB and light irradiation. This suggests that DCB can penetrate the cell membrane and enter the cell. Upon light activation, DCB in the cells can cause various cellular damages, leading to nonviable Jurkat T-cells. It appears, the nonviable cells are not caused solely by fragmentation of cellular DNA, but by other damages such as to proteins and cell membranes, or DNA alkylation. Therefore, persons exposed to DCB through environmental contamination or through tattoo piercing using DCB-containing inks must not only concern about its toxicity without exposing to light, but also its phototoxicity

  14. Mutagenic decomposition products of nitrosated 4-chloroindoles.

    Science.gov (United States)

    Brown, N K; Nguyen, T T; Taghizadeh, K; Wishnok, J S; Tannenbaum, S R

    1992-01-01

    4-Chloro-6-methoxyindole, a constituent of fava beans, forms a potent direct-acting mutagen, 4-chloro-6-methoxy-2-hydroxy-1-nitrosoindolin-3-one oxime, when nitrosated. In order to better understand the properties of this mutagen, we have studied a readily-available analog, 4-chloro-2-hydroxy-1-nitrosoindolin-3-one oxime, prepared by nitrosation of 4-chloroindole. This analog is also mutagenic, and both mutagens decompose rapidly at neutral or higher pH to yield in each case a new, less potent mutagen which then reacts further to form a nonmutagenic final product. The two products arising from 4-chloro-2-hydroxy-1-nitrosoindolin-3-one oxime, on the basis of comparison of spectroscopic and chromatographic evidence with that from authentic standards, are 4-chloro-N-nitrosodioxindole and 4-chloroisatin; those arising from 4-chloro-6-methoxy-2-hydroxy-1-nitrosoindolin-3-one oxime appear to be the corresponding 6-methoxy analogs. The interplay of these pathways with respect to net biological activity, especially under gastric conditions, remains to be described. PMID:1489931

  15. Mutagenicity assessment of acrylate and methacrylate compounds and implications for regulatory toxicology requirements.

    Science.gov (United States)

    Johannsen, F R; Vogt, Barbara; Waite, Maureen; Deskin, Randy

    2008-04-01

    Esters of acrylic acid and methacrylic acid, more commonly known as acrylates and methacrylates, respectively, are key raw materials in the coatings and printing industry, with several of its chemical class used in food packaging. The results of over 200 short-term in vitro and in vivo mutagenicity studies available in the open literature have been evaluated. Despite differences in acrylate or methacrylate functionality or in the number of functional groups, a consistent pattern of test response was seen in a typical regulatory battery of mutagenicity tests. No evidence of point mutations was observed when acrylic acid or over 60 acrylates and methacrylates were investigated in Salmonella bacterial tests or in hprt mutation tests mammalian cells, and no evidence of a mutagenic effect was seen when tested in whole animal clastogenicity and/or aneuploidy (chromosomal aberration/micronucleus) studies. Consistent with the in vivo testing results, acrylic acid exhibited no evidence of carcinogenicity in chronic rodent cancer bioassays. In contrast, acrylic acid and the entire acrylate and methacrylate chemical class produced a consistently positive response when tested in the mouse lymphoma assay and/or other in vitro mammalian cell assays designed to detect clastogenicity. The biological relevance of this in vitro response is questioned based on the non-concordance of in vitro results with those of in vivo studies addressing the same mutagenic endpoint (clastogenicity). Thus, in short-term mutagenicity tests, the acrylates and methacrylates behave as a single chemical category, and genotoxicity behavior of a similar chemical can be predicted with confidence by inclusion within this chemical class, thus avoiding unnecessary testing. PMID:18346829

  16. Studies on Mutagenicity and Teratogenicity of Sarafloxacin

    Institute of Scientific and Technical Information of China (English)

    SHEN Jian-zhong; SHEN Chuan; XIAO Xi-long; LI Jun-suo; LIU Jin-feng; ZHANG Su-xia; ZHOU Zong-can; FU Juan-ling

    2002-01-01

    Wistar rats and closed Kunming strain mice were selected to study the genetic toxicity of sarafloxacin. The results indicated that sarafloxacin had no significant toxic effect of an excreted mutagen in S. typhimurium strains, and did not induce significantly higher percentages of polyehromatic erythrocytes with micronuclei (MNPCE) in mice. No significant mutagenic activity was observed in dominant lethal assay.At 5 and 50mg/kg b.w. , sarafloxacin did not produce significant effects on the reproductive parameters of litters and fetal growth, and did not induce the teratogenic effects on fetuses. Sarafloxacin induced some toxic effects on body length and skeletal growth in fetuses of 500mg/kg b.w., but had no significant dose - response relationship among the administered dosages of sarafloxacin. The results of the genetic toxicology above indicated that no evidence showing sarafloxacin was mutagenic and potentially teratogenic for animals.

  17. STUDY OF MUTAGENICITY OF VIRAL VECTOR IN TUMOR GENETHERAPY

    Institute of Scientific and Technical Information of China (English)

    李贺书; 李殿俊; 刘旭; 李大林

    2002-01-01

    Objective: To observe the mutagenicity of retrovirus and adenovirus as transgenic vectors to evaluate the safety of transgenic tumor cells as tumor vaccines. Methods: Cells were cultured together with the virus. Then DNA and supernatant were tested for mutagenicity by means of genetic toxicological laboratory technique. Results: The results indicated that DNA and supernatant of transgenic cells had no mutagenicity through both in vivo and in vitro tests. Conclusion: The modified virus had no mutagenicity as a transgenic vector.

  18. Some problems in the evaluation of environmental mutagens

    International Nuclear Information System (INIS)

    Chemical compounds in ever-increasing variety and kind are constantly being introduced into the human environment. Some of these may adversely affect the genetic material. Such effects deserve attention not only for reasons of protecting the genetic constitution of future generations, but are also of prime and direct concern to the present in view of the striking concordance between the carcinogenic and mutagenic potential of most chemicals. That is, recent results with microbial assay systems and with Drosophila have convincingly demonstrated that the great majority of compounds capable of producing malignant transformation, are also effective in inducing genetic changes in the form of heritable mutations. A task of immediate concern thus becomes one of how such genetic and carcinogenic hazards can be avoided and how adequate regulations to minimize exposure should be formu

  19. Influence of particulate trap oxidizers on emission of mutagenic compounds by diesel automobiles.

    Science.gov (United States)

    Rasmussen, R E; Devillez, G; Smith, L R

    1989-06-01

    Diesel exhaust particles are known to contain mutagenic and carcinogenic chemicals. The aim of this study was to determine whether, and to what extent, catalytic particulate trap oxidizers on light-duty diesel engines may reduce the emission of particle-associated mutagenic chemicals into the environment. Exhaust particles were collected from Mercedes Benz and Volkswagen diesel automobiles, equipped with or without the manufacturer's exhaust traps, while running on a chassis dynamometer under specified load conditions. Exhaust particles were collected from a dilution tunnel onto 20" X 20" Teflon-coated fiberglass filters. Mutagenesis tests of dichloromethane (DCM) extracts of the particles were conducted using the Ames Salmonella bacterial test system. The mutation rate was calculated in terms of histidine revertants per mile of travel during a set of standard test cycles. With both vehicles the traps produced an 87-92% reduction in the total amount of particulate material collected by the filters. There was no significant change in the specific mutagenic activity (revertants per microgram of DCM particle extract) with or without the traps. These studies support the notion that installation of exhaust traps which reduce particulate emission on diesel-powered vehicles will also reduce the emission of particle-associated mutagenic and carcinogenic materials into the environment. PMID:2473105

  20. Induction of genetic variability in okra [Abelmoschus esculentus (L. Moench] by gamma and ems.

    Directory of Open Access Journals (Sweden)

    Peddi Sudharshan Reddy, L. K. Dhaduk

    2014-09-01

    Full Text Available An investigation was carried out to study the extent of genetic variability induced through physical and chemical mutagens in okra. Seeds of two popular okra varieties viz. GO-2 and GJO-3 were treated with 20 kR, 30 kR and 40 kR of gamma rays and 0.15% and 0.25% EMS to raise M1 generation in summer 2011 and the seed obtained from all of the M1 competitive plants were used to raise M2 in progeny rows of each of the treatments including control (the untreated one in kharif 2011.In M2 generation, increase in GCV, heritability and genetic advance was considerable under different mutagenic treatments for most of the traits studied in both varieties. In GO-2, high heritability and genetic advance was observed for some important yield contributing characters like fruit length at 40 kR and 0.25% EMS; fruit weight at 20 kR and 40 kR; and fruit yield per plant at 20, 30, 40 kR and 0.15% EMS.Similarly in GJO-3, high heritability coupled with high genetic advance was observed for fruit length at 30 kR and fruit yield per plant at 20 kR. YVMV percentage exhibited high PCV and GCV with high heritability and genetic advance in 0.25% EMS treatment in GO-2 and 40kR treatment in GJO-3. Thus selection for these characters at specific mutagenic treatments will be effective for improvement in okra.

  1. Mutagenic, Anti-Mutagenic and Cytotoxic Activities of Artediffusin (Tehranolide), in vitro, extracted from Artemisia diffusa

    OpenAIRE

    Mahboubeh Taherkhani

    2015-01-01

    Background: Artediffusin is a sesquiterpene lactone with an endoperoxide group which has been isolated from Artemisia diffusa. Artemisia has always been of great botanical and pharmaceutical interest and is useful in traditional medicines for the treatment of a variety of diseases and complaints. The aims of the present study were to evaluate the cytotoxic, mutagenic and anti-mutagenic activities of Artediffusin (Tehranolide) extracted from Artemisia diffusa. Methods: Cytotoxicity was meas...

  2. URINARY MUTAGENICITY AND COLORECTAL ADENOMA RISK

    Science.gov (United States)

    AbstractWe investigated urinary mutagenicity and colorectal adenoma risk in a clinic-based, case-control study of currently nonsmoking cases (n = 143) and controls (n = 156). Urinary organics were extracted by C18/methanol from 12-h overnight urine samples, and mutagenici...

  3. Mutagenic, Anti-Mutagenic and Cytotoxic Activities of Artediffusin (Tehranolide, in vitro, extracted from Artemisia diffusa

    Directory of Open Access Journals (Sweden)

    Mahboubeh Taherkhani

    2015-06-01

    Full Text Available Background: Artediffusin is a sesquiterpene lactone with an endoperoxide group which has been isolated from Artemisia diffusa. Artemisia has always been of great botanical and pharmaceutical interest and is useful in traditional medicines for the treatment of a variety of diseases and complaints. The aims of the present study were to evaluate the cytotoxic, mutagenic and anti-mutagenic activities of Artediffusin (Tehranolide extracted from Artemisia diffusa. Methods: Cytotoxicity was measured using a modified MTT assay on normal human lymphocytes and cancer cells. The mutagenic and anti-mutagenic activities of Artediffusin were evaluated using the Salmonella typhimurium tester strains TA98 and TA100 with and without metabolic activation S9. Results: 28μg/ml concentration of Artediffusin inactivated 77.73±0.78% of HeLa cells activity and 5600µg/ml concentration of Artediffusin inactivated 28.79±1.82% of lymphocytes activity. The maximum percentage of anti-mutagenic activity of Artediffusin was observed in the strain of S. typhimurium TA98, with the presence of metabolic activation S9. Conclusion: Artediffusin may be exploited as a natural anti-cancer and anti-mutagenic agent with low adverse side effects.

  4. Handbook of mutagenicity test procedures

    International Nuclear Information System (INIS)

    This book consists of 39 chapters. Some of the titles are: Bacillus subtilis repair test, Induced reversion using human adenovirus, The fluctuation test in bacteria, Chemical mutagenesis with diploid human fibroblasts, The specific locus test in the mouse, The bone marrow micronucleus test, and Sperm morphology in testing in mice

  5. Enhanced response of the Salmonella mutagenicity test to ionizing radiations

    International Nuclear Information System (INIS)

    Gamma-ray-induced reversions in the Ames Salmonella tester strain TA2638 have been studied for their dependence on a number of experimental parameters. It is shown that exposure to ionizing radiations soon after plating is not the procedure that yields results which correspond to those obtained in the standard utilization of the test with chemical mutagens. The ability to detect mutants is improved by irradiation 6 hr after the beginning of the incubation of the plated bacteria. This procedure has the double advantage of a markedly increased ratio of radiation-induced to spontaneous revertants and of resulting in substantial insensitivity to fluctuations in the number of bacteria initially plated. The reversion-doubling dose so obtained is 1.3 Gy; i.e., it is sufficiently small to disregard inactivation of the bacteria

  6. Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil

    Directory of Open Access Journals (Sweden)

    Vera Maria Ferrão Vargas

    1998-06-01

    Full Text Available The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX, and dichloromethane (DCM, for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction, and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3, which had considerable risk of environmental contamination by nonpolar (CX and/or moderately polar (DCM compounds. However, the values of revertants/m3 (rev/m3 were highest on the site subject to automobile exhaust (site 3 in assays without (9.56 rev/m3 and with metabolization (5.08 rev/m3. Maximum mutagenic activity was detected in the moderately polar fraction, decreasing after metabolization. Nevertheless, the nonpolar fractions (CX gave higher mutagenic activity in the presence of metabolization than in the absence of the S9 mix fraction. The responses observed for TA98NR and TA98/1,8-DNP6 strains suggest the activity of nitrocompounds.Foi investigada a atividade mutagênica de material particulado de amostras de ar coletadas em três diferentes locais dentro da área urbana da cidade de Porto Alegre, Brasil, através do ensaio Salmonella/microssoma. As amostras foram extraídas, em ultra-som, por fracionamento seqüencial de acordo com a polaridade, utilizando os solventes ciclohexano (CX e diclorometano (DCM. As diferentes frações foram testadas para mutagenicidade com as linhagens de Salmonella typhimurium TA98, em presença e ausência de ativação metabólica, e TA98NR e TA98/1,8-DNP6 em ausência de metabolização. Observou-se resposta mutagênica positiva, do tipo erro

  7. Sensitive hepatocyte-mediated assay for the metabolism of nitrosamines to mutagens for mammalian cells

    Energy Technology Data Exchange (ETDEWEB)

    Jones, C.A.; Huberman, E.

    1980-02-01

    A sensitive cell-mediated assay has been developed for testing mutagenesis in Chinese hamster V79 cells by carcinogenic nitrosamines. Mutations were characterized by resistance to ouabian and 6-thioguanine. Since V79 cells do not metabolize nitrosamines, mutagenesis in the V79 cells was tested in the presence of primary hepatocytes capable of metabolizing nitrosamines. The hepatocytes were isolated after collagenase and hyaluronidase digestion of liver slices. All seven liver carcinogens of the nine tested nitrosamines exhibited a mutagenic response in this cell-mediated assay. The potent liver carcinogens nitrosodimethylamine, nitrosodiethylamine, nitrosoethylmethylamine, and nitrosodipropylamine could be detected with doses as low as 1 ..mu..m. The noncarcinogenic nitrosodiphenylamine was not mutagenic. Nitrosomethoxymethylamine was the only nitrosamine that exhibited mutagenic activity in the absence of hepatocytes, and this activity was diminished in the presence of hepatocytes. It is suggested that the use of hepatocytes prepared by the slicing method for carcinogen metabolism and mutable V79 cells offers a highly sensitive assay for determining the mutagenic potential of carcinogenic nitrosamines and probably of other classes of hazardous chemicals occurring in the environment.

  8. MULTISTEP MUTAGENIC STRAIN IMPROVEMENT IN ASPERGILLUS CARBONARIUS TO ENHANCE PECTINASE PRODUCTION POTENTIAL

    Directory of Open Access Journals (Sweden)

    Sabika Akbar

    2013-06-01

    Full Text Available Pectinase is one of the most important commercially synthesised enzyme having its application in several industrial sectors like food and beverages, fruit clarifications etc. A.carbonarius has the capacity to produce Exo-pectinase 50 U/ml by submerged fermentation process as per the previous study. The present study describes the improvement of previously identified fungal strain Aspergillus carbonarius for enhancement of pectinase production by inducing mutations using physical and chemical mutagens. Aiming to increase the potentiality in pectinase production, the parental strain was treated for three times with four mutagens - UV irradiation, Colchicine, Hydrogen peroxide and Ethidium bromide to obtain mutants. Mutants were selected based on higher enzyme activity, improved growth rates and varied morphology with increased pectinase production. All the surviving mutants were assessed quantitatively after first mutagenic treatment. The stability of the best mutants was tested by repeating the exposures for two times to obtain 3rd generation mutants. These mutants were tested quantitatively to assess the pectinase production. Of all the best mutants E8 showed maximum activity producing 65U/ml pectinase enzyme compared to wild and sister mutants. The wild strain of A. carbonarius is a low pectinase producing organism as per literature. This strain was successfully mutated to increase the productivity rate to 1.8 fold in comparison to wild strain. This overproduction and strain stability may be due to repeated mutagenic treatments.

  9. Investigations on potential co-mutagenic effects of formaldehyde

    Energy Technology Data Exchange (ETDEWEB)

    Speit, Günter, E-mail: guenter.speit@uni-ulm.de; Linsenmeyer, Regina; Duong, Giang; Bausinger, Julia

    2014-02-15

    Highlights: • A549 cells were exposed to formaldehyde in combination with various mutagens. • Formaldehyde did not affect the induction and removal of DNA damage (comet assay). • Formaldehyde did not affect the induction of micronuclei by the mutagens tested. • The expression of the O{sup 6}-methylguanine-DNA methyltransferase was not affected. - Abstract: The genotoxicity and mutagenicity of formaldehyde (FA) has been well-characterized during the last years. Besides its known direct DNA-damaging and mutagenic activity in sufficiently exposed cells, FA at low concentrations might also enhance the mutagenic and carcinogenic effects of other environmental mutagens by interfering with the repair of DNA lesions induced by these mutagens. To further assess potential co-mutagenic effects of FA, we exposed A549 human lung cells to FA in combination with various mutagens and measured the induction and removal of DNA damage by the comet assay and the production of chromosomal mutations by the cytokinesis-block micronucleus assay (CBMN assay). The mutagens tested were ionizing radiation (IR), (±)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), N-nitroso-N-methylurea (methyl nitrosourea; MNU) and methyl methanesulfonate (MMS). FA (10–75 μM) did not enhance the genotoxic and mutagenic activity of these mutagens under the test conditions applied. FA alone and in combination with MNU or MMS did not affect the expression (mRNA level) of the gene of the O{sup 6}-methylguanine-DNA methyltransferase (MGMT) in A549 cells. The results of these experiments do not support the assumption that low FA concentrations might interfere with the repair of DNA damage induced by other mutagens.

  10. Space mutagenic effect of Trichoderma reesei

    International Nuclear Information System (INIS)

    The slant mycelia cultured with or without mutagenic agent diethyl sulfate (DS) and spores of Trichoderma reesei were loaded in the 18th returning satellite. Systematical screening showed that the life cycle and morphology of some strains had changed after space flight. After selection and domestication, 3 mutant strains with high cellulose enzyme activity were isolated. The cellulose enzyme productivities of the mutants were significantly increased more than 50%, and the mutant were generically stable. (authors)

  11. Mutagenic and cytotoxic activities of benfuracarb insecticide.

    Science.gov (United States)

    Eren, Yasin; Erdoğmuş, Sevim Feyza; Akyıl, Dilek; Özkara, Arzu

    2016-08-01

    Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem cells of Allium cepa and mutagenic effects were determined in Salmonella typhymurium Ames test by TA98 and TA100 strains with and without metabolic activation. In Allium test, 1 % DMSO was used as negative control group and 10 ppm MMS was used as positive control group. 75 ppm concentration of benfuracarb was found as EC50. In MI and mitotic phases determination study, 37.5, 75 and 150 ppm doses of benfuracarb were used. Dose-dependent cytotoxic activity was found by root growth inhibition and MI studies. It was identified that mitotic inhibition activity of benfuracarb was higher than 10 ppm MMS. In Ames test, mutagenic activity was not observed and over 200 µg/plate of benfuracarb was determined as cytotoxic to S. typhymurium strains. Benfuracarb can be called as "mitotic inhibitor" but not called as mutagen. PMID:25381170

  12. Efeito mutagênico da água natural (poço, rios Ficha e Minas Gerais, próximos à cidade de Ubiratã, Estado do Paraná, Brasil em sistema teste animal - DOI: 10.4025/actascibiolsci.v26i1.1665 Mutagenic effect of fresh water (well, rivers Ficha and Minas Gerais, close to the town of Ubiratã, Paraná, Brazil in the animal test system - DOI: 10.4025/actascibiolsci.v26i1.1665

    Directory of Open Access Journals (Sweden)

    Veronica Elisa Pimenta Vicentini

    2004-04-01

    Full Text Available O intenso desenvolvimento industrial e crescimento populacional têm promovido alterações na qualidade da água, sendo esta fonte de muitos estudos para análise desses efeitos no ser humano. Devido a contaminação dos rios por agrotóxicos, herbicidas, pesticidas e demais aditivos químicos utilizados nas lavouras, e pelas indústrias que despejam através de esgotos, resíduos de sua produção, que não são devidamente tratados, torna-se importante a investigação da atividade citotóxica e mutagênica da água dos rios. Neste estudo foi utilizado como sistema teste animal as células da medula óssea de ratos Wistar (,em>Rattus norvegicus, tratados in vivo, via gavagem, de forma subcrônica (7 dias, para investigação dos efeitos das águas do poço, e dos rios Ficha e Minas Gerais, próximos à Ubiratã, Estado do Paraná, Brasil, analisando-se o ciclo de divisão celular e os cromossomos metafásicos. A análise estatística demonstrou que as águas não alteraram o ciclo de divisão celular dos ratos Wistar e também não provocaram aumento no número de aberrações cromossômicas, mostrando não terem neste tratamento, efeito citotóxico e nem clastogênicoIntense industrial development and population growth have been altering the quality of water and innumerous studies have been undertaken to analyze their effects on humans. Due to rivers contamination with agrotoxics, herbicides, pesticides, excess of farming chemical additives and through sewers spilling not properly treated industrial waste, investigating cytotoxic and mutagenic activity of river water becomes all-important. Bone marrow cells of Wistar rats (Rattus norvegicus, treated in vivo, through gavage, in subchronic treatment (7 days, were used as experimental animal test system to investigate the effects of well water and water from the rivers Ficha and Minas Gerais, close to the municipality of Ubiratã, State of Paraná, southern Brazil. Cell division index and

  13. Exploratory research on mutagenic activity of coal-related materials

    Energy Technology Data Exchange (ETDEWEB)

    Warshawsky, D.; Schoeny, R. S.

    1980-01-01

    The following samples were found to be mutagenic for strains TA1538, TA98 and TA100 Salmonella typhimurium: ETTM-10, ETTM-11, ETTM-15, ETTM-16, and ETTM-17. ETTM-13 was marginally mutagenic for TA1537. ETTM-14 was slightly mutagenic for TA1537, TA1538, and TA98. Mutagenicity by all samples was demonstrated only in the presence of hepatic enzyme extracts (S9) which provided metabolic activation. ETTM-11 was shown to be the most mutagenic sample assayed thus far; specific activity was 2.79 x 10/sup 4/ TA98 revertants/mg sample. Fractionation by serial extractions with increasingly polar organic solvents was done at least 2 x with ETTM-10, ETTM-11, ETTM-15, ETTM-16 and ETTM-17. For some samples highly mutagenic fractions were observed.

  14. Evaluating the mutagenicity of the water-soluble fraction of air particulate matter: A comparison of two extraction strategies.

    Science.gov (United States)

    Palacio, Isabel C; Oliveira, Ivo F; Franklin, Robson L; Barros, Silvia B M; Roubicek, Deborah A

    2016-09-01

    Many studies have focused on assessing the genotoxic potential of the organic fraction of airborne particulate matter. However, the determination of water-soluble compounds, and the evaluation of the toxic effects of these elements can also provide valuable information for the development of novel strategies to control atmospheric air pollution. To determine an appropriate extraction method for assessing the mutagenicity of the water-soluble fraction of PM, we performed microwave assisted (MW) and ultrasonic bath (US) extractions, using water as solvent, in eight different air samples (TSP and PM10). Mutagenicity and extraction performances were evaluated using the Salmonella/microsome assay with strains TA98 and TA100, followed by chemical determination of water-soluble metals. Additionally, we evaluated the chemical and biological stability of the extracts testing their mutagenic potential and chemically determining elements present in the samples along several periods after extraction. Reference material SRM 1648a was used. The comparison of MW and US extractions did not show differences on the metals concentrations, however positive mutagenic responses were detected with TA98 strain in all samples extracted using the MW method, but not with the US bath extraction. The recovery, using reference material was better in samples extracted with MW. We concluded that the MW extraction is more efficient to assess the mutagenic activity of the soluble fraction of airborne PM. We also observed that the extract freezing and storage over 60 days has a significant effect on the mutagenic and analytical results on PM samples, and should be avoided. PMID:27258903

  15. Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS

    International Nuclear Information System (INIS)

    Induction of mutation by gamma rays, ethyl methane sulfonate (EMS) and their combined treatments was studied in three widely divergent genotypes of tomato, EC620176, EC620177 and Patharkutchi. A steady reduction in germination percentage, seedling height and pollen fertility occurred in M1 generation with the increasing doses / concentrations of mutagens. Combination of gamma radiation and EMS caused more damage followed by EMS treatment and gamma radiation alone in M1 generation. The LD50 dose for EC620176, EC620177 and Patharkutchi corresponded to 67.3 Gy, 290.9 Gy and 303.8 Gy gamma radiation, and 0.10%, 0.17 % and 0.38 % EMS treatment, respectively. Highest mutation frequency was resulted by gamma radiation followed by the combined mutagens and EMS treatment. Genotype and mutagen, both, influenced the production of mutants. Mutagenic efficiency of lower doses/concentrations was more compared to higher doses in producing desirable mutants. Mutagenic effectiveness of gamma radiation was the highest followed by sole EMS and combined mutagens. Gamma irradiation (50-150 Gy) was most efficient followed by 0.05-0.10 % EMS and their combination treatment in inducing wide array of macromutation in tomato. Five putative mutants with exerted stigma flower, dark green fruit, dwarf plant having pyriform fruit from Patharkutchi, multiparous cyme from EC620177 and chlorophyll deficient mutant from EC620176 that could be isolated in M2 generation hold promise for their utilization in tomato breeding programme. (author)

  16. 16. Inhibition of DNA adduct formation and mutagenic action of Trp-P-2 by chlorophyllin-chitosan in mice

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Food that humans eat every day contains mutagenic and carcinogenic compounds such as heterocyclic amines. Food also contains antimutagenic and anticarcinogenic agents. It is of great interest to seek edible, safe chemicals that could prevent the genotoxic actions of some components in food. Recently, a new antimutagenic compound,

  17. EM-31 ALTERNATIVE AND ENHANCED CHEMICAL CLEANING PROGRAM FOR SLUDGE HEEL REMOVAL - 11220

    Energy Technology Data Exchange (ETDEWEB)

    King, W.; Hay, M.; Wiersma, B.; Pennebaker, F.

    2010-12-10

    Mixtures of oxalic acid with nitric acid have been shown to be superior to oxalic acid alone for the dissolution of iron-rich High Level Waste sludge heels. Optimized conditions resulting in minimal oxalate usage and stoichiometric iron dissolution (based on added oxalate ion) have been determined for hematite (a primary sludge iron phase) in oxalic/nitric acid mixtures. The acid mixtures performed better than expected based on the solubility of hematite in the individual acids through a synergistic effect in which the preferred 1:1 Fe:oxalate complex is formed. This allows for the minimization of oxalate additions to the waste stream. Carbon steel corrosion rates were measured in oxalic/nitric acid mixtures to evaluate the impacts of chemical cleaning with these solutions on waste tank integrity. Manageable corrosion rates were observed in the concentration ranges of interest for an acid contact timescale of 1 month. Kinetics tests involving hematite and gibbsite (a primary sludge aluminum phase) have confirmed that {ge}90% solids dissolution occurs within 3 weeks. Based on these results, the chemical cleaning conditions recommended to promote minimal oxalate usage and manageable corrosion include: 0.5 wt. % oxalic acid/0.175 M nitric acid mixture, 50 C, 2-3 week contact time with agitation.

  18. Bacterial and human cell mutagenicity study of some C[sub 18]H[sub 10] cyclopenta-fused polycyclic aromatic hydrocarbons associated with fossil fuels combustion

    Energy Technology Data Exchange (ETDEWEB)

    Lafleur, A.L.; Longwell, J.P.; Marr, J.A.; Monchamp, P.A.; Thilly, W.G. (Massachusetts Institute of Technology, Cambridge (United States)); Mulder, P.P.Y.; Boere, B.B.; Cornelisse, J.; Lugtenburg, J. (Univ. of Leiden (Netherlands))

    1993-06-01

    A number of isomeric C[sub 18]H[sub 10] polycyclic aromatic hydrocarbons (PAHs), thought to be primarily cyclopenta-fused PAHs, are produced during the combustion and pyrolysis of fossil fuels. To determine the importance of their contributions to the total mutagenic activity of combustion and pyrolysis samples in which they are found, we characterized reference quantities of four C[sub 18]H[sub 10] CP-PAHs: benzol [ghi] fluoranthene (BF), cyclopenta [cd] pyrene (CPP), cyclopent [hi] acephenanthrylene (CPAP), and cyclopent [hi] acaenthrylene (CPAA). Synthesis of CPAA and CPAP is described. The availability of reference samples of these isomers also proved to be an essential aid in the identification of the C[sub 18]H[sub 10] species often found in combustion and pyrolysis samples. Chemical analysis of selected combustion and pyrolysis samples showed that CPP was generally the most abundant C[sub 18]H[sub 10] isomer, followed by CPAP and BF. CPAA was detected only in pyrolysis products from pure PAHs. We tested the four C[sub 18]H[sub 10] PAHs for mutagenicity in a forward mutation assay using S. typhimurium. CPP, BF, and CPAA were roughly twice as mutagenic as benzo[a]pyrene (BaP), whereas CPAP was only slightly active. These PAHs were also tested for mutagenic activity in human cells. In this assay, CPP and CPAA were strongly mutagenic but less active than BaP, whereas CPAP and BF were inactive at the dose levels tested. Also, the bacterial and human cell mutagenicity of CPAA and CPAP were compared with the mutagenicity of their monocyclopenta-fused analogs, aceanthrylene and acephenanthrylene. Although the mutagenicities of CPAP and acephenanthrylene are similar, the mutagenic activity of CPAA is an order of magnitude greater than that of aceanthrylene.

  19. <em>α>-Glucosidase Inhibitory Constituents from <em>Acanthopanax senticosusem> Harm Leaves

    Directory of Open Access Journals (Sweden)

    Hai-Xue Kuang

    2012-05-01

    Full Text Available A new triterpene glycoside, 3-<em>O-[(α>-L-rhamnopyranosyl(1→2]-[<em>β>-D-glucuronopyranosyl-6-<em>O>-methyl ester]-olean-12-ene-28-olic acid (1 and a new indole alkaloid, 5-methoxy-2-oxoindolin-3-acetic acid methyl ester (5 were isolated from the leaves of <em>Acanthopanax senticosusem> Harms along with six known compounds. The structures of the new compounds were determined by means of 2D-NMR experiments and chemical methods. All the isolated compounds were evaluated for their glycosidase inhibition activities and compound 6 showed significant <em>α>-glucosidase inhibition activity.

  20. Antitumoral, mutagenic and (antiestrogenic activities of tingenone and pristimerin

    Directory of Open Access Journals (Sweden)

    Juliana P. M. Gomes

    2011-12-01

    Full Text Available Cancer constitutes the second main mortality cause in the world, after cardiovascular diseases. In spite of the progresses in the chemotherapeutics treatments, many patients fail chemotherapy, mainly because of side effects or multi-drugs resistance, proving the need and importance of the research for new molecules with anticancer activity, more effective and with smaller adverse effects. Various compounds derived from plant secondary metabolites are commonly used in the chemotherapy against cancer and the natural products play an important role in the research for new molecules. Among several molecules of natural origin evaluated by MTT assay in murine tumor cell lines [breast (LM3 and lung (LP07] the quinona-methide triterpenes tingenone and pristimerin showed marked cytotoxic activity presenting IC50 around 2 and 5 µM respectively. The structure-activity relationship suggests that rings A and B containing an α, ß-unsaturated carbonyl group are essential for the observed cytotoxic activity. The interaction between these positions and acetylcisteyne residues suggests a probable mechanism of action. The in vitro mutagenic activity was also evaluated by the Salmonella microsome assay (Ames test for pristimerin and tingenone with and without metabolic activation (S9 in the strains TA98, TA97a, TA100 and TA102, none of which showed mutagenic potential in any strains. Estrogenic and anti-estrogenic activities were also studied by the e-screen assay in MCF-7 cells with negative results. The present data point to the importance of pristimerin and tingenone as representative of an emerging class of potential anticancer chemicals.

  1. Role of certain plant natural products or gamma radiation in the control of mutagenic activity of some heterocyclic amines

    International Nuclear Information System (INIS)

    The present study was designed to use ames test to evaluate the antimutagenic effect of some natural products on the lever microsomes extracted from rats and incubated with some chemical mutagens (heterocyclic compounds). Male swiss albino rats (120-140 g) were used as the source of liver microsomes. Three natural products (Nigella extract, garlic powder and sesame oil) were used to evaluate their antimutagenic activities on six heterocyclic amines. All the tested natural products exhibited their antimutagenic activities when added to the investigated heterocyclic compounds and the most effective product was nigella sativa. another group of rats was exposed to gamma-radiation (6.5 Gy) for testing the validity of ames test in quantitating mutagenicity using liver microsomes of irradiated rats. Liver microsomes from irradiated rats showed to lose ability for metabolic activation needed for heterocyclic amines to exert their mutagenic effect on salmonella typhimurium

  2. Modulation of Mutagenicity of Various Mutagens by Shrimp Flesh and Skin Extracts in Salmonella Test

    Directory of Open Access Journals (Sweden)

    S. Mehrabian

    2006-01-01

    Full Text Available Many mutagens and carcinogens may act through the generation of Reactive Oxygen Species (ROS in cells that maybe related to cancer. The carotenoid astaxanthin-the red pigment for the attractive coloration in the skin and flesh of shrimp-has attracted considerable interest in recent years because of its superior antioxidative activity to most of the hydrophobic antioxidants. According to the important role of carotenoids in human health, the main carotenoid pigment in flesh and by-products of the different shrimp species from penaeidae family-Astaxanthin-was extracted and separated by thin layer chromatography using silica gel, subsequently. The identifications were confirmed by fourier transform infrared (ET-IR spectroscopy. The effect of these extracts on mutagenicity and carcinogenicity induced by Sodium Azide and potassium permanganate was investigated in bacterial assay system, i.e., the Ames test with salmonella typhimurium TA100, TA104. These strains have a certain mutation in their histidin operon, which are related to the external histidin source. Such a mutation can be reversed through contact with a diagnostic mutagen, but in shrimp extracts prevented from effect of this mutagen in which, after repeated test series under standardized condition, the anticarcinogenic effect of flesh and skin of shrimp was proven and the shrimp skin showed stronger anticarcinogenic effect in comparison with shrimp flesh. Different shrimp species from penaeidae family indicated similar antricariongenic effects.

  3. Quantitative mammalian cell mutagenesis and a preliminary study of the mutagenic potential of metallic compounds. [Cell system used was CHO/HGPRT

    Energy Technology Data Exchange (ETDEWEB)

    Hsie, A.W.; Johnson, N.P.; Couch, D.B.; San Sebastian, J.R.; O' Neill, J.P.; Forbes, N.L.

    1978-01-01

    We have defined a set of stringent conditions required to quantify specific gene mutation in a mammalian cell system, CHO/HGPRT. Greater than 98% of the 6-thioguanine (TG)-resistant variants were shown to be deficient in the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity in Chinese hamster ovary (CHO) cells. The sensitive and quantitative nature of this assay was utilized to study the structure-activity (mutagenicity) relationship of various classes of chemicals. Mutagenicity as determined in the CHO/HGPRT assay, appears to correlate well (76/83 (92%)) with the reported animal carcinogenicity of 108 chemicals studied. The system also appears to be suitable for studying the mutagenicity and cytotoxicity of metallic compounds. We found that cis-dichlorodiammine Pt(II) (cis-Pt(NH/sub 3/)/sub 2/Cl/sub 2/) (cis-DDP), one of the widely used inorganic antitumor agents, is cytotoxic and mutagenic. Mutagenicity of cis-DDP correlates with its binding to DNA. However, trans-DDP, (Pt(NH/sub 3/)/sub 4/)Cl/sub 2/, and K/sub 2/(PtCl/sub 4/) exhibit greatly reduced biological activities. Among 14 other metals studied, we found that carcinogenic metallic compounds, such as MnCl/sub 2/, NiCl/sub 2/, and BeSO/sub 4/ are mutagenic, while non-carcinogenic compounds such as MgCl/sub 2/ and H/sub 2/SeO/sub 3/ are not. Determination of metal mutagenicity is apparently complicated by the ionic composition of the medium. This may account in part for varying results in studies of the mutagenicity of other metallic compounds. Further refinement of the assay conditions, especially with respect to the ionic environment necessary for quantifying mutagenesis of each metallic agent, is in progress.

  4. Mutagenicity profile of atmospheric particulate matter in a small urban center subjected to airborne emission from vehicle traffic and sugar cane burning.

    Science.gov (United States)

    Alves, Debora Kristina M; Kummrow, Fábio; Cardoso, Arnaldo A; Morales, Daniel A; Umbuzeiro, Gisela A

    2016-01-01

    Atmospheric particulate matter (PM) is genotoxic and recently was classified as carcinogenic to humans by the International Agency for Research on Cancer. PM chemical composition varies depending on source and atmospheric conditions. The Salmonella/microsome assay is the most used mutagenicity test and can identify the major chemical classes responsible for observed mutagenicity. The objective of this work was to characterize the mutagenicity of PM samples from a countryside city, Limeira, Brazil, which is influenced by heavy traffic and sugar cane biomass burning. Six samples of total PM were collected. Air mass backward trajectories were calculated. Organic extracts were assayed using the Salmonella/microsome microsuspension mutagenicity assay using TA98, YG1041, and TA1538, with and without metabolic activation (S9). YG1041 was the most sensitive strain and mutagenicity reached 9,700 revertants per m(3) without metabolic activation. Potency for TA1538 was higher than TA98, indicating that this strain should be considered in air mutagenicity studies. The increased response to YG1041 relative to TA98, and the decreased response with S9, suggests that nitroaromatics are the major contributors. Limeira is among the most mutagenic cities in the world. High mutagenicity in Limeira seems to occur when the air mass from the area of sugarcane production is mixed with air from the region impacted by anthropogenic activities such as traffic. An increase in the formation of nitro-polycyclic aromatic hydrocarbons may result from longer contact time between the aromatic compounds and the atmosphere with high NOx and ozone concentration, although more studies are required to confirm this hypothesis. PMID:26289646

  5. Mutagenic and carcinogenic effect of sulfur-35

    International Nuclear Information System (INIS)

    In experiments with metaphase plates from blood lymphocytes, conducted during rats' lifetime, a study was made of the mutagenic effect of 35S. Various tumors were diagnosed in the experimental animals after their death. The competitive analysis of the number of stable cytogenetic changes in lymphocytes of the experimental animals at the remote times and tumor occurrence has revealed a highly positive correlation between these indices. Both effects were severest at the highest absorbed 35S dose of less than 10 cGy

  6. In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer

    Directory of Open Access Journals (Sweden)

    Edson Luis Maistro

    2005-12-01

    Full Text Available We characterized the chemical constituents of Copaifera duckei oleoresin and used dermal application to Wistar rats to evaluated its possible mutagenic and cytotoxic activities on peripheral blood reticulocytes and bone marrow cells. Chemical characterization of the oleoresin revealed the presence of sesquiterpene hydrocarbons, an unidentified neutral diterpene and diterpene acids. To evaluate mutagenicity evaluation the rats were treated with 10, 25 and 50% of the LD50 dose of the oleoresin for three consecutive days and peripheral blood collected after 0, 24, 48 and 72 h for micronucleus analysis. The rats were humanly sacrificed 24 hours after the last treatment and chromosome preparations made using standard techniques. At the three concentrations and the three time intervals tested we found that there were no statistically significant differences in either the mean number of micronucleated reticulocytes (MNRETs or the number of chromosomal aberrations as to the negative control. However, at 25 and 50% of the LD50 dose of the oleoresin there was a significant decrease in the mitotic index (MI as compared to the negative control. Under our experimental conditions, C. duckei V11 oleoresin produced no mutagenic effects on bone marrow cells or in peripheral reticulocytes as assessed by chromosome aberrations and the micronucleus test respectively, but showed cytotoxic activity at high doses.

  7. CONTROLE QUÍMICO DE Rhizoctonia solani Kuhn EM BATATA SEMENTE CHEMICAL CONTROL OF Rhizoctonia solani Kuhn IN SEED POTATO

    OpenAIRE

    Wilson Ferreira de Oliveira; Cláudio Aparecido da Silveira

    2007-01-01

    Os fungicidas benomyl (200 g.i.a./100 kg sementes), iprodione (200 g.i.a./100 kg sementes) e pencycuron (62,5 g.i.a./100 kg de sementes) foram testados, em condições de telado, no controle de <em>Rhizoctonia solani Kuhn.em>, em batata semente da cultivar Desirée. A avaliação da percentagem de emergência damping-off aos 25 dias após o plantio, demonstrou que os fungicidas pencycuron e iprodione foram os...

  8. Controle Químico de Antracnose em Mudas de Pupunheira em Viveiro Chemical Control of Anthracnose on Peach Palm Transplants in Orchard

    Directory of Open Access Journals (Sweden)

    Rudimar Mafacioli

    2011-03-01

    Full Text Available

    A antracnose, causada pelo fungo <em>Colletotrichum loeosporioides>, é a principal doença da parte aérea da pupunheira (<em>Bactris gasipaes em>var gasipaes em viveiros de mudas no Centro-Sul do Brasil. O objetivo do trabalho foi avaliar a eficiência de novas formulações de fungicidas no controle de antracnose em mudas de pupunheira, na fase de viveiro. O ensaio foi conduzido no período de abril a agosto de 2006, com o delineamento experimental em blocos ao acaso, com cinco tratamentos com fungicidas e uma testemunha sem fungicida, com quatro repetições. As parcelas foram constituídas por dez mudas. Foram avaliados os seguintes fungicidas através de pulverização da parte aérea: piraclostrobina+epoxiconazole (0,13 + 0,05 g.L-1, tetraconazole (0,1 g.L-1, tebuconazole (0,2 g.L-1, chlorotalonil (2 g.L-1 e chlorotalonil+tiofanato metílico(1 + 0,4 g.L-1. A severidade (percentagem da área foliar doente a doença foi avaliada quinze dias após a sétima aplicação (última. Verificou-se que todos os tratamentos com fungicidas proporcionaram redução estatisticamente significativa na intensidade da doença em relação à testemunha sem fungicida (P £ 0,05. Os tratamentos com os diferentes fungicidas não diferiram significativamente entre si. Os fungicidas avaliados proporcionaram índice de controle da doença de 68 % a 78 %.  
    Leaf anthracnose, caused by <em>Colletotrichum loeosporioides>, is the most important disease of peach palm plants (<em>Bactris gasipaes em>var. gasipaes in nurseries in the Central and Southern Brazil. The objective of this study was to evaluate efficacy of some new formulations of fungicides for controlling anthracnose transplants in orchards. An assay with randomized block design was carried out, with five different fungicides and a control, with four replicates. Each replicated had 10 plants. The fungicides evaluated were: piraclostrobin + epoxiconazole

  9. 51. Mutagenicity Study of Cyclophoshpamide on Human Sperm

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Whether Cyclophoshpamide(CP) has mutagenicity on germ cell or not is paid close attention to. This paper studied the mutagenicity of CP on germ cell by adopting human sperm chromosome and micronuclus in two-cell embryo. Semen samples obtained from healthy male were liquefied、dealed with Ca2+, and exposed to four

  10. Mutagenicity of quaternary ammonium salts containing carbohydrate moieties

    International Nuclear Information System (INIS)

    Highlights: → A series of quaternary ammonium salts containing carbohydrate moieties, with configuration D-galacto, D-gluco and D-manno, was synthesized and characterized. → The quaternary ammonium salts containing carbohydrate moieties revealed potent mutagenic activities, as assessed by using the Vibrio harveyi bioluminescence mutagenicity test. → The N-[2-(D-glycopyranosyloxy)ethyl]-N,N,N-trimethylaminium salts were of the highest activity in the mutagenicity assay. → We suggest that quaternary ammonium salts may be more hazardous than previously supposed. - Abstract: Quaternary ammonium salts are widely used in industrial, agricultural, healthcare and domestic applications. They are believed to be safe compounds, with little or no health hazard to humans. However, in this report, we demonstrate that a series of newly synthesized quaternary ammonium salts containing carbohydrate moieties reveal potent mutagenic activities, as assessed by using the Vibrio harveyi bioluminescence mutagenicity test. D-Gluco- and D-galacto-derivatives were found to have a higher mutagenic potential than D-manno-derivatives. Among the former groups of compounds, the N-[2-(D-glycopyranosyloxy)ethyl]-N,N,N-trimethylaminium salts were of the highest activity in the mutagenicity assay. These results suggest that the safety of quaternary ammonium salts may be lower than previously supposed, indicating a need for testing such compounds for their mutagenicity.

  11. Mutagenicity of quaternary ammonium salts containing carbohydrate moieties

    Energy Technology Data Exchange (ETDEWEB)

    Dmochowska, Barbara [Department of Carbohydrate Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk (Poland); Piosik, Jacek; Woziwodzka, Anna [Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 Gdansk (Poland); Sikora, Karol; Wisniewski, Andrzej [Department of Carbohydrate Chemistry, University of Gdansk, Sobieskiego 18, 80-952 Gdansk (Poland); Wegrzyn, Grzegorz, E-mail: wegrzyn@biotech.univ.gda.pl [Department of Molecular Biology, University of Gdansk, Kladki 24, 80-822 Gdansk (Poland)

    2011-10-15

    Highlights: {yields} A series of quaternary ammonium salts containing carbohydrate moieties, with configuration D-galacto, D-gluco and D-manno, was synthesized and characterized. {yields} The quaternary ammonium salts containing carbohydrate moieties revealed potent mutagenic activities, as assessed by using the Vibrio harveyi bioluminescence mutagenicity test. {yields} The N-[2-(D-glycopyranosyloxy)ethyl]-N,N,N-trimethylaminium salts were of the highest activity in the mutagenicity assay. {yields} We suggest that quaternary ammonium salts may be more hazardous than previously supposed. - Abstract: Quaternary ammonium salts are widely used in industrial, agricultural, healthcare and domestic applications. They are believed to be safe compounds, with little or no health hazard to humans. However, in this report, we demonstrate that a series of newly synthesized quaternary ammonium salts containing carbohydrate moieties reveal potent mutagenic activities, as assessed by using the Vibrio harveyi bioluminescence mutagenicity test. D-Gluco- and D-galacto-derivatives were found to have a higher mutagenic potential than D-manno-derivatives. Among the former groups of compounds, the N-[2-(D-glycopyranosyloxy)ethyl]-N,N,N-trimethylaminium salts were of the highest activity in the mutagenicity assay. These results suggest that the safety of quaternary ammonium salts may be lower than previously supposed, indicating a need for testing such compounds for their mutagenicity.

  12. The mutagenicity of Gramoxone (paraquat) on different eukaryotic systems.

    Science.gov (United States)

    el-Abidin Salam, A Z; Hussein, E H; el-Itriby, H A; Anwar, W A; Mansour, S A

    1993-10-01

    The possible mutagenicity of the herbicide Gramoxone was evaluated using five different living systems: Allium cepa, Vicia faba, yeast, Drosophila melanogaster and human lymphocytes. The results indicate that Gramoxone has mutagenic activity at the cytological level in Allium cepa, Vicia faba and human lymphocytes. All doses were effective in inducing chromosomal abnormalities and a clear dose-response relationship was observed in the various cytological tests. Analysis of chromosomal abnormalities revealed that this herbicide displays clastogenic and turbagenic activities. At the gene mutation level Gramoxone induced gene conversion at the trp-5 locus and reversion at the ilv locus in Saccharomyces cerevisiae. In Drosophila melanogaster, Gramoxone proved to be mutagenic to germ cells and induced a high frequency of sex-linked recessive lethals (SLRL). At the protein level, Gramoxone had detectable mutagenic effects on the genetic background of two enzymes, Adh and Est-6. Gramoxone should be considered a mutagenic herbicide. PMID:7692291

  13. Discerning Chemical Composition and Mutagenic Effects of Soy Biodiesel PM

    Science.gov (United States)

    Exhaust particles from the combustion of traditional diesel fuel have been shown to lead to increases in adverse health effects such as impaired lung function, respiratory distress, and cardiovascular disease. This has resulted in an effort to find alternative fuels, such as soy...

  14. Characterization and validation of an in silico toxicology model to predict the mutagenic potential of drug impurities*

    Energy Technology Data Exchange (ETDEWEB)

    Valerio, Luis G., E-mail: luis.valerio@fda.hhs.gov [Science and Research Staff, Office of Pharmaceutical Science, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993–0002 (United States); Cross, Kevin P. [Leadscope, Inc., 1393 Dublin Road, Columbus, OH, 43215–1084 (United States)

    2012-05-01

    Control and minimization of human exposure to potential genotoxic impurities found in drug substances and products is an important part of preclinical safety assessments of new drug products. The FDA's 2008 draft guidance on genotoxic and carcinogenic impurities in drug substances and products allows use of computational quantitative structure–activity relationships (QSAR) to identify structural alerts for known and expected impurities present at levels below qualified thresholds. This study provides the information necessary to establish the practical use of a new in silico toxicology model for predicting Salmonella t. mutagenicity (Ames assay outcome) of drug impurities and other chemicals. We describe the model's chemical content and toxicity fingerprint in terms of compound space, molecular and structural toxicophores, and have rigorously tested its predictive power using both cross-validation and external validation experiments, as well as case studies. Consistent with desired regulatory use, the model performs with high sensitivity (81%) and high negative predictivity (81%) based on external validation with 2368 compounds foreign to the model and having known mutagenicity. A database of drug impurities was created from proprietary FDA submissions and the public literature which found significant overlap between the structural features of drug impurities and training set chemicals in the QSAR model. Overall, the model's predictive performance was found to be acceptable for screening drug impurities for Salmonella mutagenicity. -- Highlights: ► We characterize a new in silico model to predict mutagenicity of drug impurities. ► The model predicts Salmonella mutagenicity and will be useful for safety assessment. ► We examine toxicity fingerprints and toxicophores of this Ames assay model. ► We compare these attributes to those found in drug impurities known to FDA/CDER. ► We validate the model and find it has a desired predictive

  15. Characterization and validation of an in silico toxicology model to predict the mutagenic potential of drug impurities*

    International Nuclear Information System (INIS)

    Control and minimization of human exposure to potential genotoxic impurities found in drug substances and products is an important part of preclinical safety assessments of new drug products. The FDA's 2008 draft guidance on genotoxic and carcinogenic impurities in drug substances and products allows use of computational quantitative structure–activity relationships (QSAR) to identify structural alerts for known and expected impurities present at levels below qualified thresholds. This study provides the information necessary to establish the practical use of a new in silico toxicology model for predicting Salmonella t. mutagenicity (Ames assay outcome) of drug impurities and other chemicals. We describe the model's chemical content and toxicity fingerprint in terms of compound space, molecular and structural toxicophores, and have rigorously tested its predictive power using both cross-validation and external validation experiments, as well as case studies. Consistent with desired regulatory use, the model performs with high sensitivity (81%) and high negative predictivity (81%) based on external validation with 2368 compounds foreign to the model and having known mutagenicity. A database of drug impurities was created from proprietary FDA submissions and the public literature which found significant overlap between the structural features of drug impurities and training set chemicals in the QSAR model. Overall, the model's predictive performance was found to be acceptable for screening drug impurities for Salmonella mutagenicity. -- Highlights: ► We characterize a new in silico model to predict mutagenicity of drug impurities. ► The model predicts Salmonella mutagenicity and will be useful for safety assessment. ► We examine toxicity fingerprints and toxicophores of this Ames assay model. ► We compare these attributes to those found in drug impurities known to FDA/CDER. ► We validate the model and find it has a desired predictive performance.

  16. Factors influencing mutagenic mode of action determinations of regulatory and advisory agencies.

    Science.gov (United States)

    Eastmond, David A

    2012-04-28

    The determination of whether a chemical induces cancer through a mutagenic or genotoxic mechanism frequently plays an important role in evaluating the risks associated with low dose exposure. Although various approaches are employed for making mode of action decisions, a systematic investigation to identify the major factors that influence these determinations has not been performed. To accomplish this, over 40 chemical risk assessments conducted by U.S. or international regulatory agencies and organizations were reviewed to identify components that had played a significant role, either directly or indirectly, in the decision-making process. The major factors identified included the chemical properties of the agent, its metabolites and degradation products; its metabolism and toxicokinetics; genotoxic effects seen in vivo, particularly in the target organ; structural or metabolic similarities to known mutagenic or nonmutagenic chemicals; characteristics of the tumors induced in the animal bioassays; and the origin of the observed effects. The quality of the data, the specific genotoxic endpoint and its sensitivity to assay conditions and toxicity were also important considerations. In all cases, the authoritative groups used a weight-of-evidence approach and, in most cases where evaluations were conducted by more than one authoritative body, similar conclusions were reached. In summary, a critical evaluation of the data as well as expert judgment is necessary in reaching mechanism of action conclusions. These determinations should be made within the broader context of evaluating the chemical's overall toxicity and carcinogenicity. PMID:22561946

  17. Comparative mutagenicity assessment of aerosols in emissions from biofuel combustion

    Science.gov (United States)

    Mukherji, Suparna; Swain, Abhay Kumar; Venkataraman, Chandra

    This study was designed to determine the mutagenicity in extracts of aerosols generated from biofuel combustion in household cooking devices commonly used in India. Wood, dung cake and biofuel briquette were used as fuel in various stoves, including both traditional and improved stoves made of mud, fired clay and metal. The combustion aerosols of particle diameter less than 2.5 μm (PM2.5) were collected, and their organic extracts were tested for mutagenicity using the Ames Assay test with TA98 and TA100 strains of Salmonella typhimurium and studies were performed both with and without metabolic activation to account for direct and indirect acting mutagens. The measured mutagenicity emission factors, i.e., number of revertants per kg of fuel burnt, indicate that wood demonstrates significantly lower mutagenicity compared to dung cake and briquette. No significant stove effect was observed across all the fuels studied. The contribution of direct-acting mutagens was found to be greater than 70% in all cases. Such a high relative contribution of direct-acting mutagenicity has not been previously reported for biomass combustion aerosols.

  18. A methodology for assessing the impact of mutagens on aquatic ecosystems. Final report

    International Nuclear Information System (INIS)

    Assessments of impacts of hazardous agents (i.e., chemical and physical mutagens) on human health have focused on defining the effects of chronic exposure on individuals, with cancer being the main effect of concern. In contrast, impacts on ecosystems have traditionally been gauged by the assessment of near-term organism mortality, which is clearly not a useful endpoint for assessing the long-term effects of chronic exposures. Impacts on individual organisms that affect the long-term survival of populations are much more important but are also more difficult to define. Therefore, methods that provide accurate measures of sub-lethal effects that are linked to population survival are required so that accurate assessments of environmental damage can be made and remediation efforts, if required, can be initiated. Radioactive substances have entered aquatic environments as a result of research and production activities, intentional disposal, and accidental discharges. At several DOE sites, surface waters and sediments are contaminated with radioactive and mutagenic materials. The accident at the Chernobyl power station in the former Soviet Union (FSU) has resulted in the contamination of biota present in the Kiev Reservoir. This documents presents a methodology which addresses the effects of a direct-acting mutagen (radiation) on aquantic organisms by applying sensitive techniques for assessing damage to genetic material

  19. Induction of transposon TN1 translocation under the action of different mutagens

    International Nuclear Information System (INIS)

    Migration of ampicillin transposon Tn1 under normal conditions in Escherichia coli cells proceeds with low frequency (10-4 transpositions for cell). The low transposition frequency is conditioned by the transposition repression, realized by the gene-repressor in transposon structure and, probably, by other regulating genes of the bacterium-host. E. coli cell treatment by physical and chemical mutagens resulted in induction of translocation of ampicillin transposon Tn1 from plasmid RP4 into other replicons. Mitomycin C and ultraviolet radiation produced stronger inducing effect as compared to nitroso-guanidine (NG). The effect of the given mutagens on transposition Tn1 correlated with their activating capacity with respect to inducible SOS-functions of E coli. The mutation of rec A didn't influence on spontaneous Tn1 transposition, but blocked completely the induction of transposition process under mutagen effect. The relationship of inducible transposition with SOS-functions in E. coli cells, controlled by recA and lexA genes, as well as the possible role of the process in genetic microorganism variability are discussed in the paper

  20. Micronucleus test and erythropoiesis: effect of cobalt on the induction of micronuclei by mutagens.

    Science.gov (United States)

    Suzuki, Y; Shimizu, H; Nagae, Y; Fukumoto, M; Okonogi, H; Kadokura, M

    1993-01-01

    The micronucleus test is used widely as an in vivo short-term assay for potential carcinogens. In the present study, results of the micronucleus test were affected by cobalt dichloride pretreatment. Cobalt dichloride was used to induce erythropoietin, a growth factor for erythropoiesis. The increase in mutagen-induced micronucleus response following cobalt pretreatment, therefore, may have been due to a change in the rate of erythropoiesis. The greatest interaction between cobalt pretreatment and mutagen treatment for the induction of micronucleated polychromatic erythrocytes (MPCE) occurred when mice were injected with 1,1-dimethylhydrazine (DMH) 12-24 hr after pretreatment with cobalt dichloride and killed 30 hr later. Increased sensitivity of the micronucleus test was attributable to the administration of mutagen during the differentiation and multiplication of erythroblast, which is presumed to have been accelerated by pretreatment with cobalt dichloride. An increased induction of MPCE in the bone marrow by two chemicals--benzo(a)pyrene, 2-naphthylamine--was also observed following pretreatment with cobalt dichloride. PMID:8359151

  1. CHEMICALS

    CERN Multimedia

    Medical Service

    2002-01-01

    It is reminded that all persons who use chemicals must inform CERN's Chemistry Service (TIS-GS-GC) and the CERN Medical Service (TIS-ME). Information concerning their toxicity or other hazards as well as the necessary individual and collective protection measures will be provided by these two services. Users must be in possession of a material safety data sheet (MSDS) for each chemical used. These can be obtained by one of several means : the manufacturer of the chemical (legally obliged to supply an MSDS for each chemical delivered) ; CERN's Chemistry Service of the General Safety Group of TIS ; for chemicals and gases available in the CERN Stores the MSDS has been made available via EDH either in pdf format or else via a link to the supplier's web site. Training courses in chemical safety are available for registration via HR-TD. CERN Medical Service : TIS-ME :73186 or service.medical@cern.ch Chemistry Service : TIS-GS-GC : 78546

  2. Mutagenic mechanism on ion implantation of plants

    International Nuclear Information System (INIS)

    Ion beam implantation, as a new mutation technique, has been widely used in mutation breeding, and great achievements have been attained in agriculture and fermentation industry. The mechanism underlying ion beam induced mutagenic effects has been the topic of research in recent years. In this paper, we focus on the initial physical process of ion implantation into organisms, discussing that energy deposit, mass deposit and charge transfer of the implanted ions into target organisms are the main contributions to the bio-effects. Recent advances in the study of transferring of damaging signals in plant sample are also included. It has been observed that targeted ion implantation of shoot apical meristem (SAM) of Arabidopsis embryos induces damage of root apical meristem (RAM), indicating a long distant bystander effect in intact organism. Further studies showed that generation of reactive oxygen species upon ion implantation and auxin-dependent transcription processes could play important roles in the observed bystander effect. (author)

  3. Mutagenic effects of ion implantation on stevia

    International Nuclear Information System (INIS)

    Dry seeds of Stevia were implanted by 75 keV nitrogen and carbon ions with various doses. The biological effects in M1 and mutation in M2 were studied. The results showed that ion beam was able to induce variation on chromosome structure in root tip cells. The rate of cells with chromosome aberration was increased with ion beam dose. The rate of cells with chromosomal aberration was lower than that induced with γ-rays. Frequency of the mutation induced by implantation of N+ and C+ ions were higher than those induced by γ-rays. The rate of cell with chromosome aberration and in M2 useful mutation induced by implantation of C+ ion was higher than those induced by implantation of N+ ion. Mutagenic effects Feng1 x Riyuan and Riyuan x Feng2 by implantation of N+ and C+ were higher than that of Jining and Feng2

  4. Mutagenic effects of ion implanted rice seed

    International Nuclear Information System (INIS)

    Dry seeds of rice were implanted with 15∼30 keV N+, H+, Ar+ ion beam of various doses. The biological effects in M1 and mutation in M2 were studied. The results showed that ion beam could induce the variation on the chromosome structure and inhibit mitosis in root tip cell. The chromosomal aberration rate of cells tended to be increased with increase of implanted ion dose. Compared with 60Co γ-rays, ion implantation induced lower rate of cells with chromosome aberration. However, there was a similar inhibitory effect on mitosis between ion beam and γ-rays. The electrophoretic banding patterns of peroxidase enzymes were altered by both mutagens and varied. Frequency of the chlorophyll mutation implanted by ion beam was higher than that induced by γ-rays. Mutation frequencies of heading date and plant height were similar between ion beam implanting and γ-rays irradiation. (11 tabs., 2 figs.)

  5. A novel QSAR model of Salmonella mutagenicity and its application in the safety assessment of drug impurities

    Energy Technology Data Exchange (ETDEWEB)

    Valencia, Antoni; Prous, Josep; Mora, Oscar [Prous Institute for Biomedical Research, Rambla de Catalunya, 135, 3-2, Barcelona 08008 (Spain); Sadrieh, Nakissa [Office of Pharmaceutical Science, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993-0002 (United States); Valerio, Luis G., E-mail: luis.valerio@fda.hhs.gov [Office of Pharmaceutical Science, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993-0002 (United States)

    2013-12-15

    As indicated in ICH M7 draft guidance, in silico predictive tools including statistically-based QSARs and expert analysis may be used as a computational assessment for bacterial mutagenicity for the qualification of impurities in pharmaceuticals. To address this need, we developed and validated a QSAR model to predict Salmonella t. mutagenicity (Ames assay outcome) of pharmaceutical impurities using Prous Institute's Symmetry℠, a new in silico solution for drug discovery and toxicity screening, and the Mold2 molecular descriptor package (FDA/NCTR). Data was sourced from public benchmark databases with known Ames assay mutagenicity outcomes for 7300 chemicals (57% mutagens). Of these data, 90% was used to train the model and the remaining 10% was set aside as a holdout set for validation. The model's applicability to drug impurities was tested using a FDA/CDER database of 951 structures, of which 94% were found within the model's applicability domain. The predictive performance of the model is acceptable for supporting regulatory decision-making with 84 ± 1% sensitivity, 81 ± 1% specificity, 83 ± 1% concordance and 79 ± 1% negative predictivity based on internal cross-validation, while the holdout dataset yielded 83% sensitivity, 77% specificity, 80% concordance and 78% negative predictivity. Given the importance of having confidence in negative predictions, an additional external validation of the model was also carried out, using marketed drugs known to be Ames-negative, and obtained 98% coverage and 81% specificity. Additionally, Ames mutagenicity data from FDA/CFSAN was used to create another data set of 1535 chemicals for external validation of the model, yielding 98% coverage, 73% sensitivity, 86% specificity, 81% concordance and 84% negative predictivity. - Highlights: • A new in silico QSAR model to predict Ames mutagenicity is described. • The model is extensively validated with chemicals from the FDA and the public domain.

  6. A novel QSAR model of Salmonella mutagenicity and its application in the safety assessment of drug impurities

    International Nuclear Information System (INIS)

    As indicated in ICH M7 draft guidance, in silico predictive tools including statistically-based QSARs and expert analysis may be used as a computational assessment for bacterial mutagenicity for the qualification of impurities in pharmaceuticals. To address this need, we developed and validated a QSAR model to predict Salmonella t. mutagenicity (Ames assay outcome) of pharmaceutical impurities using Prous Institute's Symmetry℠, a new in silico solution for drug discovery and toxicity screening, and the Mold2 molecular descriptor package (FDA/NCTR). Data was sourced from public benchmark databases with known Ames assay mutagenicity outcomes for 7300 chemicals (57% mutagens). Of these data, 90% was used to train the model and the remaining 10% was set aside as a holdout set for validation. The model's applicability to drug impurities was tested using a FDA/CDER database of 951 structures, of which 94% were found within the model's applicability domain. The predictive performance of the model is acceptable for supporting regulatory decision-making with 84 ± 1% sensitivity, 81 ± 1% specificity, 83 ± 1% concordance and 79 ± 1% negative predictivity based on internal cross-validation, while the holdout dataset yielded 83% sensitivity, 77% specificity, 80% concordance and 78% negative predictivity. Given the importance of having confidence in negative predictions, an additional external validation of the model was also carried out, using marketed drugs known to be Ames-negative, and obtained 98% coverage and 81% specificity. Additionally, Ames mutagenicity data from FDA/CFSAN was used to create another data set of 1535 chemicals for external validation of the model, yielding 98% coverage, 73% sensitivity, 86% specificity, 81% concordance and 84% negative predictivity. - Highlights: • A new in silico QSAR model to predict Ames mutagenicity is described. • The model is extensively validated with chemicals from the FDA and the public domain. • Validation tests

  7. A contribution to the study on the mutagenicity of atmospheres

    International Nuclear Information System (INIS)

    Following a review of the literature, the genotoxic hazards of atmospheric pollutants at various locations (rural sites, motorway tolls, paint shops...) were evaluated by in vitro mutagenicity assays (Ames' test and SOS chromo-test) and analytical methods (gas chromatography and mass spectrometry). Instrumentation and procedures were developed for the sampling of volatile organic pollutants: adsorption on XAD2 followed by acetone extraction of the compounds trapped. A comparative study allowed to assess the relative mutagenic action of the volatile organic compounds and to establish a mutagenicity scale. (author)

  8. Combination effect of gamma rays, EMS and the storage before irradiation on some characteristics of M1 and M2 plants of bread wheat triticum aestivum L.(Cv. ajeeba)

    International Nuclear Information System (INIS)

    Three samples of bread-wheat CV.Ajeeba stored under baghdad prevailing room conditions since 1988,1978 and 1983 wereirradiation with gamma rays (15 Krad).They were then treated with the chemical mutagen(EMS).Seeds were sown in the field during the cultivation seasons of 1983-1985.The effects of gamma radiation,EMS,the storage periods and their interaction on some developmental stages of M1 plants were statistically significant.However,no differences were observed among M2 plants.The greatest number of variants were found among M2 plants that had originated from seeds stored since 1968,irradiated and treated with EMS.(3 tabs., 18 refs.)

  9. Effect of mutagen combined action on Chlamydomonas Reinhardtii cells. I

    International Nuclear Information System (INIS)

    The effect was investigated of single and combined actions of alkylnitrosourea derivatives (N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea) and UV-radiation on the survival of cells of Chlamydomonas reinhardtii algae in dependence on the sequence of application of mutagens and on the given conditions of cultivation following mutagen activity. In particular, the single phases were investigated of the total lethal effect, i.e., the death of cells before division and their death after division. The most pronounced changes in dependence on the sequence of application of mutagens and on the given conditions of cultivation were noted in cell death before division. In dependence on the sequence of application of mutagens, the effect of the combined action on the survival of cells changed from an additive (alkylnitrosourea + UV-radiation) to a protective effect (UV-radiation + alkylnitrosourea). (author)

  10. How stable are the mutagenic tautomers of DNA bases?

    Directory of Open Access Journals (Sweden)

    Brovarets’ O. O.

    2010-02-01

    Full Text Available Aim. To determine the lifetime of the mutagenic tautomers of DNA base pairs through the investigation of the physicochemical mechanisms of their intramolecular proton transfer. Methods. Non-empirical quantum chemistry, the analysis of the electron density by means of Bader’s atom in molecules (AIM theory and physicochemical kinetics were used. Results. Physicochemical character of the transition state of the intramolecular tautomerisation of DNA bases was investigated, the lifetime of mutagenic tautomers was calculated. Conclusions. The lifetime of the DNA bases mutagenic tautomers by 3–10 orders exceeds typical time of DNA replication in the cell (~103 s. This fact confirms that the postulate, on which the Watson-Crick tautomeric hypothesis of spontaneous transitions grounds, is adequate. The absence of intramolecular H-bonds in the canonical and mutagenic tautomeric forms determine their high stability

  11. Mutagenicity of comfrey (Symphytum Officinale) in rat liver.

    Science.gov (United States)

    Mei, N; Guo, L; Fu, P P; Heflich, R H; Chen, T

    2005-03-14

    Comfrey is a rat liver toxin and carcinogen that has been used as a vegetable and herbal remedy by humans. In order to evaluate the mechanisms underlying its carcinogenicity, we examined the mutagenicity of comfrey in the transgenic Big Blue rat model. Our results indicate that comfrey is mutagenic in rat liver and the types of mutations induced by comfrey suggest that its tumorigenicity results from the genotoxicity of pyrrolizidine alkaloids in the plant. PMID:15726100

  12. Mutagenicity of comfrey (Symphytum Officinale) in rat liver

    OpenAIRE

    MEI, N.; Guo, L.; Fu, P P; Heflich, R H; Chen, T.

    2005-01-01

    Comfrey is a rat liver toxin and carcinogen that has been used as a vegetable and herbal remedy by humans. In order to evaluate the mechanisms underlying its carcinogenicity, we examined the mutagenicity of comfrey in the transgenic Big Blue rat model. Our results indicate that comfrey is mutagenic in rat liver and the types of mutations induced by comfrey suggest that its tumorigenicity results from the genotoxicity of pyrrolizidine alkaloids in the plant.

  13. Mutagenicity assessment of aerosols in emissions from domestic combustion processes.

    Science.gov (United States)

    Canha, Nuno; Lopes, Isabel; Vicente, Estela Domingos; Vicente, Ana M; Bandowe, Benjamin A Musa; Almeida, Susana Marta; Alves, Célia A

    2016-06-01

    Domestic biofuel combustion is one of the major sources of regional and local air pollution, mainly regarding particulate matter and organic compounds, during winter periods. Mutagenic and carcinogenic activity potentials of the ambient particulate matter have been associated with the fraction of polycyclic aromatic hydrocarbons (PAH) and their oxygenated (OPAH) and nitrogenated (NPAH) derivatives. This study aimed at assessing the mutagenicity potential of the fraction of this polycyclic aromatic compound in particles (PM10) from domestic combustion by using the Ames assays with Salmonella typhimurium TA98 and TA100. Seven biofuels, including four types of pellets and three agro-fuels (olive pit, almond shell and shell of pine nuts), were tested in an automatic pellet stove, and two types of wood (Pinus pinaster, maritime pine, and Eucalyptus globulus, eucalypt) were burned in a traditional wood stove. For this latter appliance, two combustion phases-devolatilisation and flaming/smouldering-were characterised separately. A direct-acting mutagenic effect for the devolatilisation phase of pine combustion and for both phases of eucalypt combustion was found. Almond shell revealed a weak direct-acting mutagenic effect, while one type of pellets, made of recycled wastes, and pine (devolatilisation) presented a cytotoxic effect towards strain TA100. Compared to the manually fired appliance, the automatic pellet stove promoted lower polyaromatic mutagenic emissions. For this device, only two of the studied biofuels presented a weak mutagenic or cytotoxic potential. PMID:26893179

  14. Studies on mutagenicity of irradiated sugar solutions in Salmonella typhimurium

    International Nuclear Information System (INIS)

    Irradiated sugar solutions are mutagenic towards Salmonella typhimurium, the effect being dose-dependent up to 2.0 Mrad. At all doses, ribose solution exhibited greater mutagenicity than did sucrose solution. The mutagenic effect was observed only in dividing cells and appears to be directly related to the growth rate. A larger proportion of revertants was observed after incubation with irradiated sugar solution for a period of 4 h than for 24 h. Irradiation of the sugar solutions in the frozen conditions was effective in completely preventing the development of mutagenic potential. Post-irradiation storage of the sugar solutions for a prolonged period (25 weeks) also minimized their mutagenic effect. The irradiated sugar solutions gave rise to both missense and frame-shift (addition as well as deletion) types of mutation; ribose was more effective in inducing the latter type. The irradiated sugar solutions failed to show a mutagenic response in the host-mediated assay with mice as the mammalian host

  15. Anti-mutagenic activity of Salvia merjamie extract against gemcitabine.

    Science.gov (United States)

    Alanazi, Khalid Mashay

    2015-01-01

    Gemcitabine is an anti-cancer drug with clinically uses in the treatment of various neoplasms, including breast, ovarian, non-small cell lung, pancreaticand cervical cancers, T-cell malignancies, germ cell tumours, and hepatocellular carcinomas. However, it has also been reported to have many adverse effects. Naturally occurring anti-mutagenic effects, especially those of plant origin, have recently become a subject of intensive research. The present study was therefore designed to investigate the anti-mutagenic effects of Salvia merjamie (Family: Lamiaceae) plant extracts against the mutagenic effects of gemcitabine. The anti-mutagenic properties of Salvia merjamie were tested in Inbred SWR/J male and female mice bone marrow cells. The mice were treated in four groups; a control group treated with 30 mg/kg body weight gemcitabine and three treatment groups, each with 30 mg/kg body weight gemcitabine together with, respectively, 50, 100 and 150 mg/kg body weight Salvia merjamie extract. Chromosomal aberration and mitotic index assays were performed with the results demonstrating that Salvia merjamie extract protects bone marrow cells in mice against gemcitabine induced mutagenicity. This information can be used for the development of a potential therapeutic anti-mutagenic agents. PMID:25743821

  16. Development of resistance to Puccinia graminis avenae in Avena sativa by mutagen treatment

    International Nuclear Information System (INIS)

    The evaluation of over seven million M2 oat plants derived from irradiated and chemical mutagen treated seeds (about 50,000) or low-level chronically irradiated growing plants produced no new sources of useful resistance. However, preliminary results indicate that the gene Pg-16 has been transferred from the tetraploid Avena barbata L. to the hexaploid A. sativa L. by irradiation of disomic alien addition lines and monosomic alien substitution lines. This gene is highly effective and confers resistance to all but two of the known races of stem rust occurring in North America. (author)

  17. ABSENCE OF MUTAGENICITY IN THE URINE OF AUTOPSY SERVICE WORKERS EXPOSED TO FORMALDEHYDE: FACTORS INFLUENCING MUTAGENICITY TESTING OF URINE

    Science.gov (United States)

    The study examined the effect of exposure to autopsy workers to formaldehyde using urinary mutagenicity testing with Salmonella typhimurium. A matched control group was also studied. Additional studies including the recovery of histidine from urine samples, the determination of h...

  18. Mutagenic activity in disinfected waters and recovery of the potent bacterial mutagen "MX" from water by XAD resin adsorption

    Science.gov (United States)

    Backlund, Peter; Wondergem, Erik; Kronberg, Leif

    Chlorination of humic water generated mutagenic activity in the Ames test. The formation of the potent bacterial mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and mutagenic activity were favoured by acidic chlorination conditions and high chlorine doses. Chlorinated humic waters from different locations differed slightly in the level of mutagenicity as well as in the proportion of activity derived from MX. Chlorination of an industrially polluted surface water with a low content of humic material generated an approximately equal level of mutagenicity (per mg of DOC) as that of chlorinated humic water, but only a minor part (26%) of the activity could be explained by the presence of MX. The mutagenicity and the amount of MX generated were substantially lower when using combined treatment methods (ClO2+Cl2, O3+Cl2) or when substituting chlorine by monochloramine or chlorine dioxide. The recovery of MX by XAD adsorption from water acidified to pH 2 was found to be quantitative.

  19. Human mutagens: evidence from paternal exposure

    International Nuclear Information System (INIS)

    The importance of inherited mutations as a cause of human disease has been established clearly through examples of well-defined genetic anomalies, such as Down syndrome and retinoblastoma. Furthermore, it is suspected that environmental contaminants induce mutations resulting in increased risk for such defects in subsequent generations of persons exposed. The present lack of direct evidence for induced inherited genetic disorders in human beings hampers the development of risk estimation techniques for extrapolation from animal models. The most extensive prospective epidemiologic studies of inherited genetic effects have involved survivors of atomic bomb detonations and patients treated with cancer chemotherapy. In neither case has a significant elevation in inherited genetic effects or cancer been detected in the offspring of exposed individuals. Epidemiologic studies of subjects receiving chronic exposure may be confounded by the effect of maternal exposure during pregnancy. Consideration of only paternal exposure can minimize the confounding influence of teratogenicity, enhancing the resolving power of studies for inherited effects. Using this approach, retrospective (case-control) studies of childhood cancer patients have provided limited but suggestive evidence for inheritance of induced effects. Endpoints, such as congenital malformations and spontaneous abortion following paternal exposure, can also be considered as indicators of heritable mutagenic effects. For example, there is limited evidence suggesting that paternal exposure to anaesthetic gases may cause miscarriage and congenital abnormalities as a result of induced male germ cell mutations. 104 references

  20. Mutagenic effects of heavy ions in bacteria

    Science.gov (United States)

    Krasavin, E. A.; Kozubek, S.; Amirtayev, K. G.; Tokarova, B.; Bonev, M.

    The peculiarities and mechanisms of the mutagenic action of γ-rays and heavy ions on bacterial cells have been investigated. Direct mutations in the lac-operon of E. coli in wild type cells and repair deficient strains have been detected. Furthermore, the induction of revertants in Salmonella tester strains was measured. It was found that the mutation rate was a linear-quadratic function of dose in the case of both γ-rays and heavy ions with LET up to 200 keV/μm. The relative biological effectiveness (RBE) increased with LET up to 20 keV/μm. Low mutation rates were observed in repair deficient mutants with a block of SOS-induction. The induction of SOS-repair by ionizing radiation has been investigated by means of the ``SOS-chromotest'' and λ-prophage induction. It was shown that the intensity of the SOS-induction in E. coli increased with increasing LET up to 40-60 keV/μm.

  1. CHEMICAL CONTROL OF Rhizoctonia solani Kuhn IN SEED POTATO CONTROLE QUÍMICO DE Rhizoctonia solani Kuhn EM BATATA SEMENTE

    Directory of Open Access Journals (Sweden)

    Cláudio Aparecido da Silveira

    2007-09-01

    Full Text Available

    The fungicides benomyl (200 g.a.i./100 kg seeds, iprodione (200 g.a.i./l00kg seeds and pencycuron (62.5 g.a.i./100 kg seeds were evaluated, under greenhouse conditions on controlling Rhizoctonia solani Kuhn, in seed potato, with variety Desirée. The evaluation of the germination percentage and damping-off, at 25 days of age, showed that the best treatment was presented through the use of penycuron, but this fungicide had no significant difference in relation to iprodione treatment.

    Os fungicidas benomyl (200 g.i.a./100 kg sementes, iprodione (200 g.i.a./100 kg sementes e pencycuron (62,5 g.i.a./100 kg de sementes foram testados, em condições de telado, no controle de <em>Rhizoctonia solani Kuhn.em>, em batata semente da cultivar Desirée. A avaliação da percentagem de emergência damping-off aos 25 dias após o plantio, demonstrou que os fungicidas pencycuron e iprodione foram os melhores, com vantagens para o primeiro, porém sem diferirem entre si significativamente.

  2. Gamma radiation and ems treatment of black cumin cultivars for mutational bioassays

    International Nuclear Information System (INIS)

    Ten different types of chlorophyll mutations were induced in two cultivars of black cumin (Nigella sativa L.) after gamma irradiation and EMS treatment. Mutation frequency was proportional to dose for gamma rays, but not for EMS. Higher doses of gamma rays and lower concentration/duration of EMS were most efficient. Difference in the response of both cultivars due to mutagenic treatment indicate variation in the genetic architecture of the two cultivars. Origin and use of black cumin cultivars for mutagen bioassays studies are discussed

  3. Vinyl carbamate epoxide, a major strong electrophilic, mutagenic and carcinogenic metabolite of vinyl carbamate and ethyl carbamate (urethane).

    Science.gov (United States)

    Park, K K; Liem, A; Stewart, B C; Miller, J A

    1993-03-01

    Vinyl carbamate epoxide (VCO) was found to possess strong electrophilic, mutagenic and carcinogenic activities. It reacted with water at 37 degrees C and pH 7.4 (phosphate buffer) to form glycolaldehyde and several related reducing compounds; none of these products were mutagenic for Salmonella typhimurium TA1535. Under these conditions VCO had a half-life (determined chemically and mutagenically) of approximately 10.5 min. This half-life was progressively lowered by increasing concentrations of chloride ion (liver, serum and isotonic levels). This ion reacted with VCO to form chloroacetaldehyde. VCO also reacted with other nucleophiles such as glutathione, DNA and its constituent guanine and adenine bases. The purine adducts formed by VCO in DNA in vitro and in vivo were released by weak acid treatment and consisted of 7-(2'-oxoethyl)guanine and N2,3-ethenoguanine as major products with 1,N6-ethenoadenine as a minor product. VCO was a strong direct mutagen in Salmonella typhimurium TA1535 and TA100 but was only weakly active in the TA98 mutant. VCO was a stronger initiator of carcinogenesis in the skin of CD-1 mice and in the liver of infant male B6C3F1 mice than its metabolic precursors vinyl carbamate (VC) and ethyl carbamate (EC). Unlike VC and EC, VCO was a strong complete carcinogen in the skin of CD-1 mice and induced papillomas and carcinomas following repetitive administration of sub-ulcerogenic doses. VCO also exhibited some carcinogenic activity in the lungs of mice and in the s.c. and mammary tissue of female Sprague-Dawley rats. These data and those from other recent studies support the conclusion that VCO is a major strong electrophilic, mutagenic and carcinogenic metabolite of EC and VC in the mouse. PMID:8453720

  4. Characterization and Quantification of Compounds in the Hydroalcoholic Extract of the Leaves from Terminalia catappa Linn. (Combretaceae and Their Mutagenic Activity

    Directory of Open Access Journals (Sweden)

    Francisco José Mininel

    2014-01-01

    Full Text Available Terminalia is a genus of Combretaceous plants widely distributed in tropical and subtropical regions. Thus, the aim of this study was to quantify the majority compounds of the hydroalcoholic extract (7 : 3, v/v of the leaves from T. catappa by HPLC-PDA, chemically characterize by hyphenated techniques (HPLC-ESI-IT-MSn and NMR, and evaluate its mutagenic activity by the Salmonella/microsome assay on S. typhimurium strains TA98, TA97a, TA100, and TA102. The quantification of analytes was performed using an external calibration standard. Punicalagin is the most abundant polyphenol found in the leaves. The presence of this compound as a mixture of anomers was confirmed using HPLC-PDA and 1H and 13C NMR. Mutagenic activity was observed in strains TA100 and TA97a. As the extract is a complex mixture of punicalagin, its derivatives, and several other compounds, the observed mutagenicity may be explained in part by possible synergistic interaction between the compounds present in the extract. These studies show that mutagenic activity of T. catappa in the Ames test can only be observed when measured at high concentrations. However, considering the mutagenic effects observed for T. catappa, this plant should be used cautiously for medicinal purposes.

  5. Mutagenic effect of boronophenylalanine and borocaptate in neutron capture therapy

    International Nuclear Information System (INIS)

    To investigate the mutagenic effect in BNCT, CHO cells were incubated for 2 hours or 20 hours in culture medium with borocaptate sodium (BSH: Na2B12H11SH), or boronophenylalanine (BPA) prior exposure to neutrons from the heavy water facility of the Kyoto University Research Reactor (KUR) and the occurrence of mutations at the HPRT locus was measured. The mutagenicity of BSH and BPA was almost similar to the mutagenicity of 10B-boric acid at the same 10B concentration when cells were irradiated by iso-survival neutron dose. Pre-incubation to BSH for 20 hours caused an increase both in the cell killing effect and mutagenic effect in boron neutron capture therapy (BNCT) compared with pre-incubation to BSH for 2 hours. However, pre-incubation to BPA for 20 hours caused an increase in the cell killing effect but induced a decrease in mutagenic effect in BNCT compared with pre-incubation to BPA for 2 hours. (author)

  6. Genotoxic and mutagenic effects of permethrin in mice: micronuclei analysis in peripheral blood erythrocytes.

    Science.gov (United States)

    Roma, Gislaine Cristina; de Oliveira, Patrícia Rosa; Araujo, Andrea Mendez; Bechara, Gervásio Henrique; Mathias, Maria Izabel Camargo

    2012-12-01

    Pyrethroids such as permethrin are synthetic compounds widely used in the agriculture of many countries to combat plagues and in domestic products, such as acaricides. Not so long ago these chemicals were characterized as non-toxic for non-target organisms; however, recent studies have showed that these compounds could present toxic potential for many organisms. In this sense, this study presents genotoxic and mutagenic potential of permethrin administered intraperitoneally in mice under artificial conditions by the use of micronucleus assay in the peripheral blood of these animals. The mice were divided into five groups: group I = negative control (distilled water), group II = positive control (cyclophosphamide), group III = 30% of permethrin LD(50) (96 mg/kg), group IV = 50% of permethrin LD(50) (160 mg/kg), and group V = 80% of permethrin LD(50) (256 mg/kg). The peripheral blood was collected 24, 48, and 72 h after treatment. Results showed that all the tested permethrin dosages presented genotoxic and mutagenic effects 24 h after treatment, which would contradict the classification of this chemical product as moderately toxic, i.e., unable to cause damages to the cell DNA. PMID:22965619

  7. Establishing and screening of a <em>Lotus japonicusem> LORE1 mutagenized population

    DEFF Research Database (Denmark)

    Malolepszy, Anna

    Plants are constantly interacting with microorganisms, and modulate their defense responses accordingly. It is well established that legume plants are able to recognize compatible bacteria, collectively referred to as rhizobia, and establish a symbiotic interaction, which leads to the development...... of new organ – the root nodule. Within nodules, rhizobia fix atmospheric nitrogen by reducing it to ammonium, which can be absorbed by the plants. Until now, many of the important components of this pathway have been investigated and are well described. However, our knowledge about this process is...... still incomplete and we are therefore unable to fully understand the symbiotic process. This highlights the necessity for new mutagenesis and gene function tools for further investigation of the genetic mechanisms governing the symbiotic process. This work demonstrates that LORE1 can be successfully...

  8. EMS induced dwarf and high yielding mutant in yam bean (Pachyrrhizus erosus Linn.)

    International Nuclear Information System (INIS)

    The species Pachyrrhizus erosus Linn., commonly known as yam bean, is cultivated in many countries for its edible underground tubers. The young tubers can be eaten raw, cooked, or can be sliced and made into chips. As genetic variability is limited in this species, a mutation breeding programme, using physical and chemical mutagens, was initiated to induce variability in a locally adapted cultivar. Genetically pure seeds were treated with concentrations of EMS ranging from 0.25 to 2%. Evaluation of M2 raised from 1.25% treatment revealed a dwarf mutant showing a higher yield than the control. This mutant attained a height of 20 cm only in M3 as against 82 cm in the controls. Though the starch content of the tuber and rotenone content in the seeds were the same as that in the control, protein content was slightly higher and the sugar content slightly less

  9. Atributos químicos e atividade microbiana em solos convertidos de savana para plantios de Acacia mangium Willd em Roraima. = Chemical attribuites and microbial activity in soils converted to savanna for plantations of Acacia mangium Willd. in Roraima, Brazil.

    Directory of Open Access Journals (Sweden)

    Moisés Mourão Júnior

    2011-04-01

    Full Text Available A atividade microbiana tem sido empregada na avaliação de solos manejados, sendo um indicador importante na caracterização de solos alterados. Nesse sentido, objetivou-se com o presente trabalho avaliar o efeito de plantios de Acacia mangium com diferentes idades sobre os atributos químicos e atividade microbiana em solos representativos da Savana do Estado de Roraima. Os plantios de A. mangium de três e cinco anos encontravam-se em áreas de LATOSSOLO AMARELO Distrófico e os de quatro e seis anos em ARGISSOLO AMARELO Distrófico. As áreas próximas aos plantios com as mesmas condições de solos e com cobertura vegetal primária (savana natural constituiram as testemunhas. Foram amostrados os horizontes A e B sendo avaliados os atributos químicos dos solos e a atividade microbiana. A pobreza química e elevadasaturação por alumínio (%m dos solos estudados influenciaram significativamente nos menores valores de C-CO2 evoluído pela atividade microbiana. A maior atividade microbiana ocorreu em áreas plantadas independentemente do tipo de solo, em função das melhores condições químicas. Os maiores valores de C-CO2 evoluído correlacionaram-se melhor com os teores de carbono orgânico e matéria orgânica do solo. = Microbial activity has been used in the evaluation of soils, an important indicator in the characterization of alteredsoils. Therefore, this study has as aim to evaluate the effect of replacing natural vegetation to plantations of Acacia mangium onmicrobial activity in soils of savannas in Roraima state. The areas studied consisted of plantations of acacia with three and fiveyears in Yellow Latosol with four and six years in yellow Alfisols. Areas near the plantations with the same conditions of soiland vegetation (natural savanna were considered control. Were sampled the A and B horizons, corresponding depths 0-30 and 30-60 cm respectively, with three replications for a total of 36 experimental units. The results

  10. Mutagenicity studies on alcohol extracts from gamma-irradiated potatoes

    International Nuclear Information System (INIS)

    The alcohol extracts freshly prepared from gamma-irradiated potatoes were examined for their mutagenic activity in bacterial and mammalian cell systems. Negative results were obtained from all following test systems: Mutation assays with Salmonella typhimurium His- strains such as TA 100, TA 98, TA 1535, TA 1537, and streptomycin-dependent mutant (SM sup(d)) strain, TA 100 - 10, inductests with Escherichia coli strains, K 12 GY 5027 and K 12 C600, chromosomal aberration tests with Chinese hamster cells in culture, as well as micronucleus tests in mice. In addition, no difference in the mutagenic activities was found between extracts prepared from the irradiated and the unirradiated potatoes, suggesting that no mutagenic substance was produced in potatoes following gamma-irradiation. (author)

  11. Sources of mutagenic activity in urban fine particles

    International Nuclear Information System (INIS)

    Samples were collected during the winter of 1984-1985 in the cities of Albuquerque, New Mexico and Raleigh, North Carolina as part of a US Environmental Agency study to evaluate methods to determine the emission sources contributing to the mutagenic properties of extractable organic matter (EOM) present in fine particles. Data derived from the analysis of the composition of these fine particles served as input to a multi-linear regression (MLR) model used to calculate the relative contribution of wood burning and motor vehicle sources to mutagenic activity observed in the extractable organic matter. At both sites the mutagenic potency of EOM was found to be greater (3-5 times) for mobile sources when compared to wood smoke extractable organics. Carbon-14 measurements which give a direct determination of the amount of EOM that originated from wood burning were in close agreement with the source apportionment results derived from the MLR model

  12. Sources of mutagenic activity in urban fine particles

    International Nuclear Information System (INIS)

    Samples were collected during the winter of 1984-1985 in the cities of Albuquerque, NM and Raleigh NC as part of a US Environmental Protection Agency study to evaluate methods to determine the emission sources contributing to the mutagenic properties of extractable organic matter (EOM) present in fine particles. Data derived from the analysis of the composition of these fine particles served as input to a multi-linear regression (MLR) model used to calculate the relative contribution of wood burning and motor vehicle sources to mutagenic activity observed in the extractable organic matter. At both sites the mutagenic potency of EOM was found to be greater (3-5 times) for mobile sources when compared to wood smoke extractable organics. Carbon-14 measurements which give a direct determination of the amount of EOM that originated from wood burning were in close agreement with the source apportionment results derived from the MLR model

  13. OECD Guidelines for the Testing of Chemicals, Test No. 226: Predatory mite (<em>Hypoaspis (Geolaelaps) aculeiferem>) reproduction test in soil

    DEFF Research Database (Denmark)

    Römbke, L. Becker, B. Dark, Th. Moser, N. Halsall, W. Powley, A. Ruf, C. Scholer, E. Smit, P. Wege, N. Zenz m.fl., J.; Krogh, Paul Henning

    2008-01-01

    replicates for each test concentrations and six to eight control replicates, of 10 animals each, are recommended. At 20 oC, the test lasts 14 days after introducing the females, which usually allows the control offspring to reach the deutonymph stage. The number of surviving females (mortality < 20% for a...... valid test) and the number of juveniles per test vessel (at least 50 for a valid test) are determined. The fecundity of the mites exposed to the test substance is compared to that of controls in order to determine the ECx (e.g. EC10, EC50) or the No Observed Effect Concentration (NOEC). Any observed......This Test Guideline describes a method to assess the effects of chemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps) aculeifer Canestrini (Acari: Laelapidae). It can be used for water soluble or insoluble substances, but not with volatile substances...

  14. Avaliação da mutagenicidade e antimutagenicidade de um biopolímero extraído do microorganismo Agrobacterium radiobacter em camundongos Swiss Assessment of mutagenicity and antimutagenicity of a biopolymer extracted from the microorganism Agrobacterium radiobacter in mice

    OpenAIRE

    Milka Selestina Primo; Caroline Maria Calliari; Raúl Jorge Hernan Castro-Gómez; Mariana de Oliveira Mauro; Mário Sérgio Mantovani; Rodrigo Juliano Oliveira

    2010-01-01

    A presente pesquisa avaliou a ação mutagênica e antimutagênica de um biopolímero de glucose extraído da Agrobacterium radiobacter (Biopolímero de Agrobacterium radiobacter). O experimento foi realizado com camundongos Swiss machos divididos em oito grupos. O tratamento com o biopolímero foi realizado por gavage em dose única concomitante a uma dose de solução tampão fosfato nos grupos de avaliação da mutagenicidade, ou ao agente indutor de danos no DNA, ciclofosfamida, na concentração de 50 m...

  15. Reflections on the Origins and Evolution of Genetic Toxicology and the Environmental Mutagen Society

    Energy Technology Data Exchange (ETDEWEB)

    Wassom, John S. [Oak Ridge National Laboratory (Retired); Malling, Heinrich V. [National Institute of Environmental Health Sciences (NIEHS); Sankaranarayanan, K. [Leiden University; Lu, Po-Yung [ORNL

    2010-01-01

    This article traces the development of the field of mutagenesis and its metamorphosis into the research area we now call genetic toxicology. In 1969 this transitional event led to the founding of the Environmental Mutagen Society (EMS). The charter of this new Society was to encourage interest in and study of mutagens in the human environment, particularly as these may be of concern to public health. As the mutagenesis field unfolded and expanded, the lexicon changed and new wording appeared to better describe this evolving area of research. The term genetic toxicology was coined and became an important subspecialty of the broad area of toxicology. Genetic toxicology is now set for a thorough reappraisal of its methods, goals, and priorities to meet the challenges of the 21st Century. To better understand these challenges, we have revisited the primary goal that the EMS founders had in mind for the Society s main mission and objective, namely, the quantitative assessment of genetic (hereditary) risks to human populations exposed to environmental agents. We also have reflected upon some of the seminal events over the last 40 years that have influenced the advancement of the genetic toxicology discipline and the extent to which the Society s major goal and allied objectives have been achieved. Additionally, we have provided suggestions on how EMS can further advance the science of genetic toxicology in the postgenome era. Chronicling all events and publications that influenced the development of the mutagenesis and genetic toxicology research area for this article was not possible, but some key happenings that contributed to the field s development have been reviewed. Events that led to the origin of EMS are also presented in celebration of the Society s 40th anniversary. Any historical accounting will have perceived deficiencies. Key people, publications, or events that some readers may feel have had significant impact on development of the subject under review may

  16. Constituents from <em>Vigna em>vexillata> and Their Anti-Inflammatory Activity

    Directory of Open Access Journals (Sweden)

    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of <em>Vigna em>genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of<em> V. vexillata em>demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of <em>V. vexillataem>. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  17. Determining effective radiation mutagen dose for garlic (Allium sativum L.)

    International Nuclear Information System (INIS)

    This study was carried out to get database for future garlic mutation breeding studies. For this aim, 0, 5, 10, 15, 20, 25 and 30 Gy doses of Cs137 (gamma-ray) were applied on garlic cloves as a physical mutagen. 50 cloves were used for each dose. Sixty days after treatment, germination rate and shoot development of cloves were determined. The Effective Mutagen Dose (ED50) was calculated by regression analyses. According to the results, 4.455 Gy dose was found to be effective as ED50. (author)

  18. Structure of γ-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.

    Science.gov (United States)

    Greenberg, Charles H; Kollman, Justin; Zelter, Alex; Johnson, Richard; MacCoss, Michael J; Davis, Trisha N; Agard, David A; Sali, Andrej

    2016-06-01

    Modeling protein complex structures based on distantly related homologues can be challenging due to poor sequence and structure conservation. Therefore, utilizing even low-resolution experimental data can significantly increase model precision and accuracy. Here, we present models of the two key functional states of the yeast γ-tubulin small complex (γTuSC): one for the low-activity "open" state and another for the higher-activity "closed" state. Both models were computed based on remotely related template structures and cryo-EM density maps at 6.9Å and 8.0Å resolution, respectively. For each state, extensive sampling of alignments and conformations was guided by the fit to the corresponding cryo-EM density map. The resulting good-scoring models formed a tightly clustered ensemble of conformations in most regions. We found significant structural differences between the two states, primarily in the γ-tubulin subunit regions where the microtubule binds. We also report a set of chemical cross-links that were found to be consistent with equilibrium between the open and closed states. The protocols developed here have been incorporated into our open-source Integrative Modeling Platform (IMP) software package (http://integrativemodeling.org), and can therefore be applied to many other systems. PMID:26968363

  19. Identification of chemical signatures of gunshot residues in different fabrics; Identificacao de assinaturas quimicas em residuos de disparos de arma de fogo em diferentes alvos

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, Joao Carlos Dias de

    2010-07-01

    The modern forensic science goes hand in hand with scientific research. The forensic scientists are faced every day with many cases requiring the analysis of residues from firing gun (gunshot residues). This works describes the development of a methodology to determine chemical signatures of shots from a firearm, by measuring the concentrations of Pb, Ba e Sb in the residues from these shots deposited near the entrance hole of bullets, based on the technique with high resolution inductively coupled plasma mass spectrometry (HRICP-MS). Shots were performed on five types of target-fabrics and collected testimonies from regions close to the entrance hole of projectiles. The results showed that the method enabled us to identify and distinguish the residues of the .38 caliber revolver and pistols .40 and 9mm caliber. The use of ternary graphs as a tool for data analysis helped to identify specific patterns of distribution of blank samples and gunshot residues deposited after firing revolvers and pistols. The methodology enabled the assignment of the origin of the shot through the confirmation of the residues collected also from the hands of shooters. As a result the methodology in police procedures and aims to add a valuable contribution to forensic investigations. (author)

  20. Improved mutagen-testing systems in mice. Progress report, 1 June 1976--31 August 1977

    International Nuclear Information System (INIS)

    Results are reported from studies on the production of chromosomal inversion by chemical treatment or irradiation of sperm in mice and to detect inversions by observing high frequencies of first meiotic anaphase bridges of their sons or by using chromosomal banding techniques to detect inverted segments cytologically. For each new inversion, which is either of considerable length or which has particularly useful experimental properties, we will determine its linkage group, mark it genetically, if possible, or place it with a genetically marked homologous chromosome, and study its cytological, physiological, and anatomical effects. The inversions are being used to construct recessive lethal testing systems for estimating mutational loads in populations exposed to radiation or either proved or potential chemical mutagens, to mark and maintain induced lethals for analysis of their potential dominant effects on fitness, and to study other basic problems in mammalian genetics

  1. 40 Years of the Salmonella Mutagenicity Assay: Implications for 21st Century Toxicology

    Science.gov (United States)

    The Salmonella (Ames) mutagenicity assay was developed and introduced by Bruce Ames and colleagues in 1971. Since then, it has become the standard assay for hazard identification of mutagens worldwide. It is a first-tier test for mutagenic activity in the pharmaceutical and chemi...

  2. REVIEW OF THE SALMONELLA TYPHIMURIUM MUTAGENICITY OF BENZIDINE, BENZIDINE ANALOGUES, AND BENZIDINE-BASED DYES

    Science.gov (United States)

    The mutagenicity of benzidine analogues (including benzidine-based dyes) was reviewed with a primary emphasis on evaluating results of the Salmonella/microsome mutagenicity assay. Many of these amines are mutagenic in tester strains TA98 and TA100 but require exogenous mammalian ...

  3. Efeitos do processamento químico e da esterilização em Óxido de etileno em osso cortical e esponjoso de ratas: estudo com microscopia de luz e eletrônica de varredura Effects of chemical processing and oxide ethylene sterilization on cortical and cancellous rat bone: a light and electron scanning microscopy study

    Directory of Open Access Journals (Sweden)

    Marcello Teixeira Castiglia

    2009-02-01

    Full Text Available OBJETIVO: Avaliar, sob o ponto de vista microscópico, modificações estruturais do osso esponjoso e cortical, após serem submetidos a processamento químico e esterilização em óxido de etileno. MÉTODOS: Amostras de osso esponjoso e cortical foram obtidas de fêmures de ratas albinas jovens (Wistare separadas em quatro grupos contendo osso cortical e esponjoso: I- Fragmentos secos em estufa; II- Fragmentos secos em estufa e esterilizados em óxido de etileno; III- Fragmentos processados quimicamente; IV- Fragmentos processados quimicamente e esterilizados em óxido de etileno. Metade desse material foi analisada em microscópio eletrônico de varredura e, a outra metade, em microscopia de luz convencional. RESULTADOS: Houve preservação da morfologia geral das amostras em todos os grupos. Nos grupos submetidos ao processamento químico houve melhor preservação do conteúdo celular, enquanto que naqueles submetido ao óxido de etileno houve amalgamação fibrilar. CONCLUSÃO: O tratamento com óxido de etileno causou amalgamação das fibrilas possivelmente em decorrência do efeito do calor e o tratamento químico contribuiu para melhor preservação da estrutura óssea.OBJECTIVE: To evaluate, under microscopic examination, the structural changes displayed by the trabecular and cortical bones after being processed chemically and sterilized by ethylene oxide. METHODS: Samples of cancellous and cortical bones obtained from young female albinus rats (Wistar were assigned to four groups according to the type of treatment: Group I-drying; Group II-drying and ethylene oxide sterilization; III-chemical treatment; IV-chemical treatment and ethylene oxide sterilization. Half of this material was analyzed under ordinary light microscope and the other half using scanning electron microscopy. RESULTS: In all the samples, regardless the group, there was good preservation of the general morphology. For samples submitted to the chemical processing

  4. Assessment of mutagenic damage by monofunctional alkylating agents and gamma radiation in haploid and diploid frogs, Xenopus laevis

    International Nuclear Information System (INIS)

    Adult male South African clawed frogs, Xenopus laevis, were mutagenized by 3-day immersion in aqueous solutions of ethyl methanesulfonate (EMS), diethyl nitrosamine (DEN), or ethyl nitrosourea (ENU), or by acute exposure to gamma radiation. They were then spawned repeatedly at 2-week intervals with untreated females, and embryonic survival of the progeny was used to assess genetic damage. Recessive lethal effects were assessed from reduced survival of androgenetic haploid progeny. Neither recessive nor dominant lethal effects were obtained after exposure to 100 mg/liter EMS or 2 g/liter DEN. At 250 mg/liter EMS, peak dominant lethality occurred 3-5 weeks after treatment. Most embryos hatched, but many were abnormal and died shortly after hatching. Haploid survival was significantly reduced over a broader period, from 1 to 13 weeks after mutagenesis. Treatment with 75 mg/liter ENU produced effects similar to the 250-mg/liter EMS mutagenesis. At 400 mg/liter EMS, the frequency and severity of the effects on both diploid and haploid embryos were increased over the lower dose. Gamma irradiation at 1500 R produced effects similar to the 400-mg/liter mutagenesis, except that peak dominant lethality extended from 1 to 7 weeks

  5. "Cut holes and sink 'em": chemical weapons disposal and cold war history as a history of risk

    OpenAIRE

    Müller, Simone

    2016-01-01

    Using the incident of the scuttling of the USS Le Baron Russell Briggs, loaded with roughly 22,000 tons of outdated chemical weapons in 1970, this contribution extrapolates how, why, and when in the United States chemical weapons that had been produced as the ultimate answer to the risk of nuclear war became reframed as a risk themselves. The analysis settles on how questions of knowing and not-knowing about potentialities of future events influenced these re-negotiation processes between the...

  6. 61. STUDY ON MUTAGENICITY OF PENICILLIUM DIGITATUM

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Penicillium digitatum (P. digitatum) is a pathogenic fungus mildewed fruits and its process products. There were some events of poisoning on the clinic because fruits and its process products mildewed by the fungi are eaten. A lot of reports were about the study on acute poisoning on this hand. But there were few reports about the study on genetoxicity, and therefor we determined the mutagenicity of P. digitatum with different methods to provide scientific basis for prevent the effect of human genetoxicity. METHODS: ① The preparation of extract from P. digitatum: Preponderant fungus separated from mildewed fruits was incubated in the Czapak's medium for two weeks, and then it was extracted with CHCL2 and evaporated. It was dissolved by dimethylsulfoxide (DMSO) for use. ② Bacterial reverse mutation assay: The assay was without S9 mix. E. coli ND-160 strain was used in the assay. The assay set up negative control, positive control and test group, The test group contained four concentrations (3.125 mg/plate, 6.25 mg/plate, 12.5 mg/plate and 25 mg/plate). ③ Micronucleus assay of polychromatic erythrocytes (PCE) in mice bone marrow. The assay was individed into negative control group (0.9% NaCl, 20 ul/mice), positive control group (cyclophosphamide, CP, 30 mg/kg B.W.) and test group (extract, 250 mg/kg B.W.). ④ Unscheduled DNA synthesis (UDS) assay in primary cells of lung and liver of rat. Fresh cells separated from lung and liver were incubated. There were three groups in the test. They were negative control group SO, 1%(v/v)], positive control group (10-7 mol/L 3-MC for lung cell; 10-7 mol/L HN2. HCL for liver cell). The radioactivity of cells that were treated was detected. The result showed unscheduled incorporation index, and the index represented the level of UDS. ⑤ Mutation assay in E. coli K12 infA gene. Using E. coli K12 strain as mutation target, partial infA gene was amplified by PCR, and the overlapping fragments were cloned into PGEN-T vector

  7. Mutagenicity, cytotoxicity and phytotoxicity evaluation of biodegraded textile effluent by fungal ligninolytic enzymes.

    Science.gov (United States)

    Bilal, Muhammad; Iqbal, Munawar; Hu, Hongbo; Zhang, Xuehong

    2016-01-01

    Colored effluents from the textile industry have led to severe environmental pollution, and this has emerged as a global issue. The feasibility of ligninolytic enzymes for the detoxification and degradation of textile wastewater was investigated. Ganoderma lucidum crude ligninolytic enzymes extract (MnP 717.7, LiP 576.3, and Laccase 323.2 IU/mL) was produced using solid-state culture using wheat bran as substrate. The biodegradation treatment efficiency was evaluated on the basis of degradation and detoxification of textile effluents. Standard bioassays were employed for mutagenicity, cytotoxicity and phytotoxicity evaluation before and after biodegradation. The degradation of Masood Textile, Kalash Textile, Khyber Textile and Sitara Textile effluents was achieved up to 87.29%, 80.17%, 77.31% and 69.04%, respectively. The biochemical oxygen demand, chemical oxygen demand, total suspended solids and total organic carbon were improved considerably as a result of biodegradation of textile effluents, which were beyond the permissible limits established by the National Environmental Quality Standards before treatment. The cytotoxicity (Allium cepa, hemolytic, Daphnia magna and brine shrimp), mutagenicity (Ames TA98 and TA100) and phytotoxicity (Triticum aestivum) tests revealed that biodegradation significantly (P < 0.05) detoxifies the toxic agents in wastewater. Results revealed that biodegradation could possibly be used for remediation of textile effluents. However, detoxification monitoring is crucial and should always be used to evaluate the bio-efficiency of a treatment technique. PMID:27191553

  8. 28. Annual Meeting of the European Environmental Mutagen Society

    International Nuclear Information System (INIS)

    The 28th Annual Meeting of the European Environmental Mutagen Society took place in Salzburg from September 7th till September 11th, 1998. A lot of presentations also dealt with many radiation effects on cells, chromosomal aberrations and genetic effects caused by radioactive irradiation. In vivo and in vitro experiments concerning radiation injuries and carcinomas are analyzed. (Cecil)

  9. Mutagenicity of Diesel and Soy Biodiesel Exhaust Particles

    Science.gov (United States)

    Mutagenicity Of Diesel And Soy Biodiesel Exhaust Particles E Mutlua,b' SH Warrenb, PP Matthewsb, CJ Kingb, B Prestonc, MD Haysb, DG Nashb,ct, WP Linakb, MI Gilmourb, and DM DeMarinib aUniversity of North Carolina, Chapel Hill, NC bU.S. Environmental Agency, Research Triangle Pa...

  10. Flavonoids and alkenylbenzenes: mechanisms of mutagenic action and carcinogenic risk

    NARCIS (Netherlands)

    Rietjens, I.M.C.M.; Boersma, M.G.; Woude, van der H.; Jeurissen, S.M.F.; Schutte, M.E.; Alink, G.M.

    2005-01-01

    The present review focuses on the mechanisms of mutagenic action and the carcinogenic risk of two categories of botanical ingredients, namely the flavonoids with quercetin as an important bioactive representative, and the alkenylbenzenes, namely safrole, methyleugenol and estragole. For quercetin a

  11. Adverse reproductive outcomes from exposure to environmental mutagens

    Czech Academy of Sciences Publication Activity Database

    Šrám, Radim; Binková, Blanka; Dejmek, Jan; Perreault-Darney, S.; Rubeš, J.; Selevan, S. G.; Topinka, Jan

    Bangkok : Organizing Committee, 1998, s. 59. [International Conference on Environmental Mutagens in Human Populations. Bangkok (TH), 29.11.1998-04.12.1998] R&D Projects: GA MŽP ZZ/340/1/97 Subject RIV: DN - Health Impact of the Environment Quality

  12. Occurrence of biflavonoids in Clusiaceae: chemical and pharmacological aspects; Ocorrencia de biflavonoides em Clusiaceae: aspectos quimicos e farmacologicos

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Rafaela Oliveira [Universidade Federal Rural do Rio de Janeiro, Seropedica, RJ (Brazil). Dept. de Quimica; Carvalho, Mario Geraldo de [Universidade Federal do Rio de Janeiro (NPPN/UFRJ), RJ (Brazil). Nucleo de Pesquisa em Produtos Naturais; Silva, Tania Maria Sarmento da, E-mail: mgeraldo@ufrrj.br [Universidade Federal Rural de Pernambuco, Recife, PE (Brazil). Dept. de Ciencias Moleculares

    2012-07-01

    This work describes the biflavonoids found in species of Clusiaceae, particularly the genera Garcinia and Calophyllum, emphasizing the importance of these metabolites as chemical markers of this family, their contribution to the pharmacological potential of these species, besides the promising potential of these compounds in the search for new drugs. (author)

  13. Review of the Séralini <em>et alem>. (2012 publication on a 2-year rodent feeding study with glyphosate formulations and GM maize NK603 as published online on 19 September 2012 in Food and Chemical Toxicology

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2012-10-01

    Full Text Available

    On 19 September 2012, Séralini <em>et al.em> published online in the scientific journal Food and Chemical Toxicology a publication describing a 2-year feeding study in rats investigating the health effects of genetically modified (GM maize NK603 with and without Roundup WeatherMAX® and Roundup® GT Plus alone (both are glyphosate-containing plant protection products.  EFSA was requested by the European Commission to review this publication and to identify whether clarifications are needed from the authors. EFSA notes that the Séralini <em>et al.em> (2012 study has unclear objectives and is inadequately reported in the publication, with many key details of the design, conduct and analysis being omitted. Without such details it is impossible to give weight to the results. Conclusions cannot be drawn on the difference in tumour incidence between the treatment groups on the basis of the design, the analysis and the results as reported in the Séralini <em>et al.em> (2012 publication. In particular, Séralini <em>et al.em> (2012 draw conclusions on the incidence of tumours based on 10 rats per treatment per sex which is an insufficient number of animals to distinguish between specific treatment effects and chance occurrences of tumours in rats.  Considering that the study as reported in the Séralini <em>et al.em> (2012 publication is of inadequate design, analysis and reporting, EFSA finds that it is of insufficient scientific quality for safety assessment. Therefore EFSA, concludes that the Séralini <em>et al.em> study as reported in the 2012 publication does not impact the ongoing re-evaluation of glyphosate, and does not see a need to reopen the existing safety evaluation of maize NK603 and its related stacks. EFSA will give the authors of the Séralini <em>et al.em> (2012 publication the opportunity to provide further information on their study to EFSA.

  14. Mutagenicity Evaluation of Irradiated Indian Mackerel in Swiss Mice. Dominant Lethal Assay and Micronucleus Test

    International Nuclear Information System (INIS)

    To evaluate the potential mutagenic effects of irradiated (150 krad) Indian mackerel (Rastrelliger kanagurta) in somatic and germ cells of mice, a micronucleus test and dominant lethal assay were employed. Four to five-weeks-old Swiss male mice were randomly assigned to four groups. Two groups were fed irradiated or unirradiated mackerel at 35% level dry weight for 16 weeks. Twenty males from each group were used for dominant lethal assay. The males in the positive control group were given 200 mg/kg of ethyl methane sulphonate (EMS) intraperitoneally about 24 hours before pairing with the females. The number of dead implantations (deciduomas and dead embryos) showed no significant difference among the groups fed on stock rations or irradiated or unirradiated mackerel diets at any stage of the test period. There was also no increase in the pre-implantation or total lethality. As expected, EMS-treated mice showed a highly significant increase in the dead implantation rates and a reduction of the live implantations during the postmeiotic phase of spermatogenesis. For micronucleus tests mackerel diets and stock rations were continued for a period of 21 weeks. The positive control group mice were given 80 mg/kg hycanthone methane sulphonate, intraperitoneally twice, 30 and 6 h before killing. Animals fed on irradiated or unirradiated mackerel diets or stock rations showed no significant differences either in the frequency of micronuclei or the ratio of polychromatic to normochromatic cells. (author)

  15. Mutagenic treatments towards increasing the frequency of day-neutral mutations and standardization of procedures for tissue culture, in potato

    International Nuclear Information System (INIS)

    Various chemical mutagens and gamma radiation have been used on single dormant eyes and true seeds with a view to finding effective mutagenic treatment for the induction of day-length neutral mutants in potato using an effective screening technique for the isolation of day-length neutral mutants. Sodium meta bisulphite (SMS) was found to be an efficient mutagen in inducing mutations for this trait in true seeds although the same concentrations, when used for treating the single tuber eyes proved lethal. Pre-soaking the seeds for 24 hrs prior to treatment with 0.0025M SMS gave highest frequency of the mutants followed by 48 hrs presoaking, indicating a sensitive stage during the cell cycle in true seeds. Other mutagen treatments gave different frequencies of mutations. The highest frequency of day-length neutral mutants was observed when seeds irradiated with 40 Kr of gamma radiation were treated with 0.05M hydrazinium dichloride solution. Screening procedures have also been standardised with the development of synethetic media for the isolation of biochemical mutants at the true seed level. Initial efforts have yielded mutants resistant to LD100 doses of ethionine. Another aspect of the study was to develop a proper potato callus culture technique. A medium has been developed to produce and maintain callus from potato leaf strips. Efforts on the regeneration of shoot and roots from callus, have so far lead to differentiation of callus to form roots. The ultimate aim of these studies is to develop plantlets from single cell which would form the units of mutation induction and isolation. (author)

  16. Genotoxicity studies in semiconductor industry. 1. In vitro mutagenicity and genotoxicity studies of waste samples resulting from plasma etching

    Energy Technology Data Exchange (ETDEWEB)

    Braun, R.; Huettner, E.M.; Merten, H.; Raabe, F. (Institute of Plant Genetics and Crop Plant Research, Gatersleben (Germany))

    1993-07-01

    Solid waste samples taken from the etching reactor, the turbo pump, and the waste air system of a plasma etching technology line in semiconductor production were studied as to their genotoxic properties in a bacterial repair test, in the Ames/Salmonella microsome assay, in the SOS chromotest, in primary mouse hepatocytes, and in Chinese hamster V79 cell cultures. All three waste samples were found to be active by inducing of unscheduled DNA-synthesis in mouse hepatocytes in vitro. In the bacterial rec-type repair test with Proteus mirabilis, waste samples taken from the turbo pump and the vacuum pipe system were not genotoxic. The waste sample taken from the chlorine-mediated plasma reactor was clearly positive in the bacterial repair assay and in the SOS chromotest with Escherichia coli. Mutagenic activity was demonstrated for all samples in the presence and absence of S9 mix made from mouse liver homogenate. Again, highest mutagenic activity was recorded for the waste sample taken from the plasma reactor, while samples collected from the turbo pump and from the waste air system before dilution and liberation of the air were less mutagenic. For all samples chromosomal damage in V79 cells was not detected, indicating absence of clastogenic activity in vitro. Altogether, these results indicate generation of genotoxic and mutagenic products as a consequence of chlorine-mediated plasma etching in the microelectronics industry and the presence of genotoxins even in places distant from the plasma reactor. Occupational exposure can be expected both from the precipitated wastes and from chemicals reaching the environment with the air stream.

  17. Anti mutagenesis of chemical modulators against damage induced by reactor thermal neutrons

    International Nuclear Information System (INIS)

    The mutations are changes in the genetic information whether for spontaneous form or induced by the exposure of the genetic material to certain agents, called mutagens: chemical or physical (diverse types of radiations). As well as exist a great variety of mutagens and pro mutagens (these last are agents which transform themselves in mutagens after the metabolic activation). Also several chemical compounds exist which are called antimutagens because they reduce the mutagens effect. The C vitamin or ascorbic acid (A A) presents antimutagenic and anti carcinogenic properties. On the other hand a sodium/copper salt derived from chlorophyll belonging to the porphyrin group (C L) contains a chelated metal ion in the center of molecule. It is also an antioxidant, antimutagenic and anti carcinogenic compound, it is called chlorophyllin. The objective of this work is to establish if the A A or the C L will reduce the damages induced by thermal and fast reactor neutrons. (Author)

  18. Chemicals additive used in silages <>.Aditivos químicos utilizados en el ensilaje Aditivos químicos utilizados em silagens

    Directory of Open Access Journals (Sweden)

    Mikael Neumann

    2010-05-01

    Full Text Available

    The objective was to describe the characteristics referent to chemical additives with emphasis to the preservatives or inhibiters of microorganism development which act on the ensiled material. Ensilage is a method used in the preservation of forage quality during the storage process, not increasing nutritional values besides those comprehended on forage. Non conventional forage species, which are not maize or sorghum, when ensiled required special precautions, since the possibility of loss during the stages of the ensilage process do not indicate that every productive and qualitative potential of the culture should be maintained in the resulting silage. Due to occurrence of nutritive loss of the ensiled material, it may have necessity of utilization of additives. Various additives have been used with several purposes, as the urea (increase on content of crude protein and neutral detergent fiber, the calcium carbonate (reducer of fermentation process and calcium fountain, sodium benzoate (conservation of carbohydrates, effect reducer on ethanol concentration and leavens inhibition, sodium pyrosulphite (cellular respiration inhibition and bactericide, sodium hydroxide (reduction on the components of the cellular wall and increase on values of “<em>in vitroem>” digestibility of dry matter formic acid (dehydrate effects and bactericide and the formol (bacteriostatic action and protection of protein adversely action of microorganisms. The employment of chemical additives which inhibit the microorganism development is explained in critical cases as material with low content of dry matter and/or low content of soluble carbohydrates, difficulty on compaction of the ensiled mass and when the impediment of garner to present high oxygen percentage in inner silo.

    El objetivo fue describir las principales características relativas los aditivos químicos, con énfasis a los preservativos o inhibidores de lo

  19. Effect of mutagens on seed germination in Gloriosa superba LINN

    International Nuclear Information System (INIS)

    Seeds of Gloriosa superba were irradiated with gamma rays ranging from (1 kR to 35 kR) at an interval of 5 kR. Lower doses (1 kR and 5 kR) were stimulative while higher doses had inhibitory effect on seed germination. The highest percentage of germination was 29.5 and it was observed at 5 kR as compared to 24 per cent in control. Various concentrations of EMS (0.25 to 1 per cent) and dES (0.05 to 1 per cent) were used for duration of 4 hours. In the treated seeds the maximum percentage of germination was 23 and 17.5 per cent at 0.25 EMS and 0.05 per cent dES respectively. Whereas in control it was 25 per cent. There was proportionate decrease in germination percentage with an increase in concentration of both the chemicals. (author). 3 tables, 3 figs., 38 refs

  20. Eficiência e custo do controle químico da mancha de alternaria em tangor murcote Efficiency and cost of chemical control of alternaria brown spot

    Directory of Open Access Journals (Sweden)

    Adimara Bentivoglio Colturato

    2009-09-01

    Full Text Available A mancha de alternaria, causada por Alternaria alternata f. sp. citri, afeta tangelos Minneola, tangerinas Dancy, tangores Murcote e, menos freqüentemente, tangelos Orlando, tangerinas Novas, Lees e Sunburst. Esta doença causa desfolha grave, queda de frutos e manchas nas frutas. O objetivo deste trabalho foi estabelecer o melhor fungicida e a melhor dose para o controle da mancha marrom de alternaria. O delineamento experimental foi de parcelas subdivididas em blocos, com 10 tratamentos principais e 3 doses (subparcelas, com 5 repetições. Foram feitas 5 aplicações, com intervalo de 15 dias. Os tratamentos foram: azoxystrobin, pyraclostrobin, trifloxystrobin, trifloxystrobin + tebuconazole (2 aplicações seguido de 3 aplicações de mancozeb, difenoconazole, trifloxystrobin + propiconazole, iprodione, trifloxystrobin + propineb (2 aplicações seguido de 3 aplicações de oxicloreto de cobre, oxicloreto de cobre + óleo e testemunha. Simultaneamente foram feitas avaliações de incidência e número de lesões por folha. Ao surgimento dos frutos foram avaliadas a incidência em frutos e a produtividade em Kg/ha. Todos os tratamentos foram superiores à testemunha quanto a produtividade. Entre os produtos utilizados o tratamento com trifloxystrobin + propiconazole foi rentável comparando-se custo e produtividade.Alternaria brown spot, caused by Alternaria alternata sp. citri, attacks with more intensity the Tangelos Minneola, tangerine Dancy, and Murcotts, and with less intensity the tangelos Orlando and the tangerinas Novas, Lees and Sunburst. This disease causes severe defoliation and drop or necrotic spots in the fruits. The aim of this work was to evaluated the chemical control of brown spot, and to define the most appropriate dosage of fungicide to control it. The experimental design was split-spot, with ten treatments and 3 doses of fungicides, with five replicates, the fungicides were: azoxystrobin, pyraclostrobin, trifloxystrobin

  1. Evaluation of the genotoxic and mutagenic potentials of phytotherapic and homeopathic solutions of Euphorbia tirucalli Lineu (Aveloz

    Directory of Open Access Journals (Sweden)

    Carla Holandino

    2011-07-01

    Full Text Available Introduction: Euphorbia tirucalli Lineu, commonly known as Aveloz, is a very common plant found in tropical regions [1]. The ingestion or contact with its latex causes symptoms such as vomiting and diarrhea, pallor, skin irritation, hepatotoxicity as well as carcinogenesis [2]. Moreover, the Aveloz latex is also responsible for a few important activities against some infectious and neoplastic diseases. Aveloz latex phytochemical composition may vary according to seasonal aspects and geographic location [3], and it is used either orally or topically in traditional medicine. Popularly known as an antitumoral agent (breast, prostate, lung, kidney, it is used not only in Brazil, but in several other countries. According to the literature, the latex could have a dual behaviour, activating or inhibiting tumoral events [3-6]. However, there are few reports discussing these mechanisms. Besides, the mutagenic and genotoxic potentials of phytochemical and homeopathic Aveloz have not yet been described. Several experimental methods have been used to evaluate the mutagenic and genotoxic effects, such as Inductest, the Ames test and the chromotest. Objective: This study aims to evaluate the genotoxic and mutagenic potentials of Aveloz latex and Aveloz phytotherapic and homeopathic solutions. Methodology: In this study, Aveloz 5 and 30cH are prepared according to Brazilian Homeopathic Pharmacopoeia [7], from Aveloz latex collected in the Center for Natural Products Research (NPPN at the Universidade Federal do Rio de Janeiro [8]. The Aveloz phytochemical solution was prepared following the doses used in folk medicine: 2 drops diluted in 250ml of water and 2 drops diluted in 25 ml of water. All test solutions were submitted to the following methodologies: (a Inductest: assesses the ability of physical or chemical agents to promote lysogenic induction as a response to DNA damage in lysogenic bacteria; (b The Ames test: uses indicator strains of Salmonella

  2. Variabilidade espacial de atributos químicos em latossolo e argissolos Spatial variability of chemical attributes in latosol and argisol

    OpenAIRE

    Rafael Montanari; Gener Tadeu Pereira; José Marques Júnior; Zigomar Menezes de Souza; Renato Junqueira Pazeto; Lívia Arantes Camargo

    2008-01-01

    O trabalho teve como objetivo caracterizar a variabilidade espacial de atributos químicos de Latossolos e Argissolos, sob cultivo de cana-de-açúcar em áreas com variações na forma do relevo. No presente estudo utilizou-se duas áreas, sendo uma em Latossolo em pedoforma convexa (158ha) e a outra em Argissolo na pedoforma linear (172ha). Foi coletada amostra de solo em malha na profundidade de 0,00-0,50m, realizando-se análise química de cada ponto amostrado. Os maiores coeficientes de variação...

  3. Ausência de mutagenicidade e antimutagenicidade do extrato obtido das flores do ipê roxo [Tabebuia impetiginosa (Mart. ex DC. Standl.] Absence of mutagenicity and antimutagenicity of the extract obtained from the flowers of "ipê roxo" Tabebuia impetiginosa (Mart. ex DC. Standl

    Directory of Open Access Journals (Sweden)

    J.A. Lourenço

    2010-12-01

    Full Text Available A Tabebuia impetiginosa, conhecida popularmente como ipê-roxo, é uma planta nativa das florestas tropicais chuvosas da América do Sul e Central. Componentes químicos obtidos da casca têm mostrado efeito terapêutico, como antiinflamatório, antifúngico e antibacteriano. Porém, pela falta de dados na literatura, pouco se sabe sobre os efeitos do extrato das flores. Assim, o objetivo do presente trabalho foi avaliar o potencial mutagênico e antimutagênico do extrato obtido das flores da T. impetiginosa, em três diferentes concentrações (100, 300 e 500 mg kg-1 p.c. pelo teste do micronúcleo. Para o teste de mutagenicidade, a doxorrubicina (DXR, 90 mg kg-1 p.c. foi utilizada como indutor de danos no DNA e para o teste de antimutagenicidade, os tratamentos com o extrato foram realizados simultaneamente com este agente químico. O sangue periférico dos animais foi coletado 24 horas após os tratamentos. A comparação da frequência de eritrócitos policromáticos (PCEs em 400 eritrócitos/animal entre os diferentes grupos não demonstrou qualquer citotoxicidade do extrato. Em relação às frequências de micronúcleos em PCEs (PCEMNs, não foram observadas diferenças significativas entre os grupos tratados com as diferentes concentrações de extrato e o controle negativo. Da mesma forma, todos os grupos de animais que receberam os tratamentos simultâneo do extrato (100, 300 ou 500 mg kg-1 p.c. com a DXR, apresentaram valores de PCEMNs muito próximos quando comparados com os dados observados no grupo de animais que recebeu somente a DXR. Esses resultados apresentados indicam ausência de efeito mutagênico e antimutagênico do extrato obtido das flores da T. impetiginosa em sistema teste in vivo.T. impetiginosa, known as "ipê-roxo", is a plant native to tropical rain forests of Central and South Americas. Chemical compounds obtained from its bark have shown anti-inflammatory, antifungal and antibacterial therapeutic effect. However

  4. CL 64, 855, a potent do anti-Trypanosoma cruzi drug, is also mutagenic in the salmonella/microsome assay

    Directory of Open Access Journals (Sweden)

    R. C. C. Ferreira

    1986-03-01

    Full Text Available The nitroimidazole-tiadiazole derivative CL 64,855 (2-amino-5-(1-methyl-5-nitro-2-imidazolyl-1,3,4-thiadiazole, a potent anti-trypanosomal drug, was assayed in a short-term bacterial mutagenicity test with Salmonella typhimurium strains TA 98, TA 100 and TA 102. Results indicate that CL 64,855 is a potent frameshift mutagen detected by strains TA 98 and TA 102. CL 64,855 was able to revert the indicators strains at concentrations as low as 0.1 µg/plate. Metabolic activation experiments with rat liver microsomal fractions did not increase the mutagenic action of Cl 64,855.O derivado nitroimizadole-tiadizol CL 64.855 (2-amino-5-(1-metil-5-nitro-2-imidazoli-1, 3, 4-tiadiazol, um potente agente tripanomicida, foi submetido a um ensaio mutagênico bacteriano com as linhagens indicadoras de Salmonella typhimurium TA 98, TA 100 e TA 102. Os resultados indicaram que o CL 64.855 é um potente mutagênico tipo troca de referencial detectado pelas linhagens TA 98 e TA 102. O CL 64.855 foi capaz de reverter as linhagens indicadoras em concentrações tão baixas quatro 0,1microng/placa. Ativação metabólica com frações microssomais de fígado de rato foram incapazes de aumentar a ação mutagênica do CL 64.855.

  5. Counteracting quasispecies adaptability: extinction of a ribavirin-resistant virus mutant by an alternative mutagenic treatment.

    Directory of Open Access Journals (Sweden)

    Celia Perales

    Full Text Available BACKGROUND: Lethal mutagenesis, or virus extinction promoted by mutagen-induced elevation of mutation rates of viruses, may meet with the problem of selection of mutagen-resistant variants, as extensively documented for standard, non-mutagenic antiviral inhibitors. Previously, we characterized a mutant of foot-and-mouth disease virus that included in its RNA-dependent RNA polymerase replacement M296I that decreased the sensitivity of the virus to the mutagenic nucleoside analogue ribavirin. METHODOLOGY AND PRINCIPAL FINDINGS: Replacement M296I in the viral polymerase impedes the extinction of the mutant foot-and-mouth disease virus by elevated concentrations of ribavirin. In contrast, wild type virus was extinguished by the same ribavirin treatment and, interestingly, no mutants resistant to ribavirin were selected from the wild type populations. Decreases of infectivity and viral load of the ribavirin-resistant M296I mutant were attained with a combination of the mutagen 5-fluorouracil and the non-mutagenic inhibitor guanidine hydrocloride. However, extinction was achieved with a sequential treatment, first with ribavirin, and then with a minimal dose of 5-fluorouracil in combination with guanidine hydrochloride. Both, wild type and ribavirin-resistant mutant M296I exhibited equal sensitivity to this combination, indicating that replacement M296I in the polymerase did not confer a significant cross-resistance to 5-fluorouracil. We discuss these results in relation to antiviral designs based on lethal mutagenesis. CONCLUSIONS: (i When dominant in the population, a mutation that confers partial resistance to a mutagenic agent can jeopardize virus extinction by elevated doses of the same mutagen. (ii A wild type virus, subjected to identical high mutagenic treatment, need not select a mutagen-resistant variant, and the population can be extinguished. (iii Extinction of the mutagen-resistant variant can be achieved by a sequential treatment of a

  6. Pollen genetic markers for detection of mutagens in the environment

    International Nuclear Information System (INIS)

    To utilize and exploit pollen for in situ mutagen monitoring, screening and toxicology, the range of genetic traits in pollen must be identified and analyzed. To be useful for the development of mutagen detection systems proteins should be: (1) activity stainable or immunologically identifiable in the pollen, (2) the products of one to three loci; and (3) gametophytic and nuclear in origin. Several proteins, including alcohol dehydrogenase in maize, which meet these criteria are discussed. The waxy locus in barley and maize which controls starch deposition for pollen screening and mutant detection. Thirty waxy mutant lines, induced by sodium azide and gamma-rays are characterized for spontaneous and induced reversion frequencies, allelism, karyotype, amylose content, and UDPglucose glucosyltransferase (waxy gene product) activity. Twelve mutant alleles are being mapped by recombinant frequencies

  7. THE BNL ASTD FIELD LAB - NEAR - REAL - TIME CHARACTERIZATION OF BNL STOCKPILED SOILS TO ACCELERATE COMPLETION OF THE EM CHEMICAL HOLES PROJECT

    International Nuclear Information System (INIS)

    As of October 2001, approximately 7,000 yd3 of stockpiled soil remained at Brookhaven National Laboratory (BNL) after the remediation of the BNL Chemical/Animal/Glass Pits disposal area. The soils were originally contaminated with radioactive materials and heavy metals, depending on what materials had been interred in the pits, and how the pits were excavated. During the 1997 removal action, the more hazardous/radioactive materials were segregated, along with, chemical liquids and solids, animal carcasses, intact gas cylinders, and a large quantity of metal and glass debris. Nearly all of these materials have been disposed of. In order to ensure that all debris was removed and to characterize the large quantity of heterogeneous soil, BNL initiated an extended sorting, segregation, and characterization project directed at the remaining soil stockpiles. The project was co-funded by the Department of Energy Environmental Management Office (DOE EM) through the BNL Environmental Restoration program and through the DOE EM Office of Science and Technology Accelerated Site Technology Deployment (ASTD) program. The focus was to remove any non-conforming items, and to assure that mercury and radioactive contaminant levels were within acceptable limits for disposal as low-level radioactive waste. Soils with mercury concentrations above allowable levels would be separated for disposal as mixed waste. Sorting and segregation were conducted simultaneously. Large stockpiles (ranging from 150 to 1,200 yd3) were subdivided into manageable 20 yd3 units after powered vibratory screening. The 1/2-inch screen removed almost all non-conforming items (plus some gravel). Non-conforming items were separated for further characterization. Soil that passed through the screen was also visually inspected before being moved to a 20 yd3 ''subpile.'' Eight samples from each subpile were collected after establishing a grid of four quadrants: north, east, south and west, and two layers: top and

  8. QSAR screening of 70,983 REACH substances for genotoxic carcinogenicity, mutagenicity and developmental toxicity in the ChemScreen project

    DEFF Research Database (Denmark)

    Wedebye, Eva Bay; Dybdahl, Marianne; Nikolov, Nikolai Georgiev;

    2015-01-01

    The ChemScreen project aimed to develop a screening system for reproductive toxicity based on alternative methods. QSARs can, if adequate, contribute to the evaluation of chemical substances under REACH and may in some cases be applied instead of experimental testing to fill data gaps for...... were used to reach overall predictions of substances with potential effects with the following result: 6.5% genotoxic carcinogens, 16.3% mutagens, 11.5% developmental toxicants. These results are similar to findings in earlier QSAR and experimental studies of chemical inventories, and illustrate how...... QSAR predictions may be used to identify potential genotoxic carcinogens, mutagens and developmental toxicants by high-throughput virtual screening....

  9. Behavioral screening for cocaine sensitivity in mutagenized zebrafish

    OpenAIRE

    Darland, Tristan; Dowling, John E.

    2001-01-01

    Understanding the molecular basis of addiction could be greatly aided by using forward genetic manipulation to lengthen the list of candidate genes involved in this complex process. Here, we report that zebrafish exhibit cocaine-induced conditioned place preference. In a pilot screen of 18 F2 generation families of mutagenized fish, we found three with abnormally low responses to cocaine. This behavior was inherited by the F3 generation in a manner that suggests th...

  10. Differences in mutagenic and recombinational DNA repair in enterobacteria

    International Nuclear Information System (INIS)

    The incidence of recombinational DNA repair and inducible mutagenic DNA repair has been examined in Escherichia coli and 11 related species of enterobacteria. Recombinational repair was found to be a common feature of the DNA repair repertoire of at least 6 genera of enterobacteria. This conclusion is based on observations of (i) damage-induced synthesis of RecA-like proteins, (ii) nucleotide hybridization between E. coli recA sequences and some chromosomal DNAs, and (iii) recA-negative complementation by plasmids showing SOS-inducible expression of truncated E. coli recA genes. The mechanism of DNA damage-induced gene expression is therefore sufficiently conserved to allow non-E. coli regulatory elements to govern expression of these cloned truncated E. coli recA genes. In contrast, the process of mutagenic repair, which uses umuC+ umuD+ gene products in E. coli, appeared less widespread. Little ultraviolet light-induced mutagenesis to rifampicin resistance was detected outside the genus Escherichia, and even within the genus induced mutagenesis was detected in only 3 out of 6 species. Nucleotide hybridization showed that sequences like the E. coli umuCD+ gene are not found in these poorly mutable organisms. Evolutionary questions raised by the sporadic incidence of inducible mutagenic repair are discussed

  11. Mutagenicity and genotoxicity of coal fly ash water leachate

    Energy Technology Data Exchange (ETDEWEB)

    Chakraborty, R.; Mukherjee, A. [University of Calcutta, Calcutta (India). Dept. of Botany

    2009-03-15

    Fly ash is a by-product of coal-fired electricity generation plants. The prevalent practice of disposal is as slurry of ash and water to storage or ash ponds located near power stations. This has lain to waste thousands of hectares of land all over the world. Since leaching is often the cause of off-site contamination and pathway of introduction into the human environment, a study on the genotoxic effects of fly ash leachate is essential. Leachate prepared from the fly ash sample was analyzed for metal content, and tested for mutagenicity and genotoxicity. Analyses of metals show predominance of the metals - sodium, silicon, potassium, calcium, magnesium, iron, manganese, zinc, and sulphate. The Ames Salmonella mutagenicity assay, a short-term bacterial reverse mutation assay, was conducted on two-tester strains of Salmonella typhimurium strains TA97a and TA102. For genotoxicity, the alkaline version of comet assay on fly ash leachate was carried in vitro on human blood cells and in vivo on Nicotiana plants. The leachate was directly mutagenic and induced significantconcentration-dependent increases in DNA damage in whole blood cells, lymphocytes, and in Nicotiana plants. The comet parameters show increases in tail DNA percentage (%), tail length (mu m), and olive tail moment (arbitrary units). Our results indicate that leachate from fly ash dumpsites has the genotoxic potential and may lead to adverse effects on vegetation and on the health of exposed human populations.

  12. Evaluation of Mutagenicity of Three Eugenol-Containing Materials

    Directory of Open Access Journals (Sweden)

    Narlan Sumawinata

    2015-10-01

    Full Text Available Eugenol containing materials are still widely used, both by the lay people or by the dentist. Professionally it is used to relieve dental pain by placing it in the cavity, or as a mixture for temporary filling, temporary cementation, and root canal sealer. Eugenol, however, is also known to be toxic to the tissue, and aflatoxin, a substance known to be mutagenic, has been found in material labeled as clove oil. The purpose of this study was to investigate the safety of these materials, in particular the mutagenicity. The materials tested were imported eugenol material (95.8% eugenol and local eugenol material (99.5%, both used by dentists and clove-oil material (6.8% eugenol used by the lay people. These materials were assayed in Ames test using Salmonella typhimarium TA 1535 and TA 1537 strain without S-9 mixture. It was shown that all revertants were below the negative control of the test. It was concluded that neither the eugenol used by the dentist nor the clove oil used for suppressing the dental pain by the lay people showed mutagenicity to Salmonella typhimurium TA 1535 and TA 1537 strain.

  13. Planejamento e programação da produção em plantas multipropósito operando em batelada na indústria química Short term planning and scheduling for multipurpose batch chemical plants

    Directory of Open Access Journals (Sweden)

    Luis Gimeno Latre

    2000-12-01

    Full Text Available Neste artigo, considera-se o problema de programação da produção a curto prazo em plantas químicas multipropósito operando em batelada, em que a produção da planta é determinada pela demanda a ser atendida. Nesta situação, a capacidade da planta não está bem definida porque depende do mix de produção. Propõe-se uma fase de planejamento que tem como objetivo a determinação do número de bateladas de cada tarefa necessárias para atender a produção, bem como a respectiva janela de tempo de processamento. Estas janelas permitem a análise do carregamento dos processadores e da factibilidade do plano, ou seja, o atendimento das datas de entrega, através de ferramentas desenvolvidas na área de Busca Orientada por Restrições. Esta análise é feita para uma atribuição fixa de tarefas a processadores introduzida pelo usuário. O sistema fornece informações para orientar o usuário na criação de diferentes cenários de atribuição. O resultado da fase de planejamento é formado por um conjunto de janelas de processamento que diminuem sensivelmente a dimensão do problema de programação da produção, como é discutido para duas abordagens: programação mista (Mixed Integer Linear Programming - MILP e Simulated Annealing.Short term scheduling in multipurpose batch chemical plants is specially complicated by the fact that plant capacity is not well defined due to the multipurpose nature of equipment units, allowing different routes. In order to reduce problem dimension, as well as allowing to analyze problem feasibility prior to spending time solving a large scheduling problem, a planning phase is proposed. At this level the objective is to determine a set of processing time windows for the batches necessary to fulfill final products' demand. The time windows allow analyzing equipment units load and plan feasibility in terms of satisfying final products due dates. To accomplish those tools from the area of Constrained

  14. The pH dependence of the mutagenicity of methyl benzimidazol-2-yl carbamate (MBC) towards Aspergillus nidulans (Eidam) Winter and Cladosporium cucumerinum Ellis & Arth.

    Science.gov (United States)

    Nirenberg, H I; Speakman, J B

    1981-01-01

    The ability of methyl benzimidazol-2-yl carbamate (MBC) to induce point mutations to carboxin and MBC resistance in Aspergillus nidulans (Eidam) Winter and Cladosporium cucumerinum Ellis & Arth. was dependent upon the pH value of the agar medium into which it had been incorporated. The relevance of this in relation to testing chemicals for a possible mutagenic activity with microorganisms is discussed. PMID:7010148

  15. Mutagenic screening of some commonly used medicinal plants in Nigeria.

    Science.gov (United States)

    Akintonwa, Alade; Awodele, Olufunsho; Afolayan, Gbenga; Coker, Herbert A B

    2009-09-25

    The uses of medicinal plants have always been part of human culture. The World Health Organization estimates that up to 80% of the world's population relies on traditional medicinal system for some aspect of primary health care. However, there are few reports on the toxicological properties of most medicinal plants especially, their mutagenicity and carcinogenicity. Therefore, this research is to determine the mutagenic potentials of Morinda lucida [Oruwo (Root)], Azadirachta indica [Dongoyaro (Leaf)], Terapluera tetraptera [Aridan (Fruit)], Plumbago zeylanica [Inabiri (Root)], Xylopia aethiopica [Erunje (Fruit)], Newbouldia laevis [Akoko (Leaf)], Alstonia boonei [Ahun (Bark)], Enantia chlorantha [Awopa (Bark)], and Rauvolfia vomitoria [Asofeyeje (Root)] using the Allium cepa Linn. model and the modified Ames assay. Allium cepa model was used to determine the mean root length, mitotic index and chromosomal aberrations effects of these plants on onion bulbs using 0.1, 1, 5 and 10mg/ml concentration of the plant extracts. The modified Ames test which is a modification of the standard Ames test as described by Ames et al. [Ames, B.N., McCann, J., Yamasaki, E., 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutation Research 31, 347-364] was done using Escherichia coli (0157:H7) that has the phenotypic characteristics of glucose and lactose fermentation, motile, urease negative, indole positive and citrate negative. The results obtained from Allium cepa assay showed increasing root growth inhibition with increased concentration, decreasing mitotic index with increased concentration and chromosomal aberrations. The modified Ames test showed an alteration in the biochemical characteristics of Escherichia coli (0157:H7) for all plants except Rauvolfia vomitoria and Plumbago zeylanica. Three of the medicinal plants altered at least three of the normal biochemical characteristics thus demonstrating mutagenic

  16. Salmonella mutagenicity assessment of airborne particulate matter collected from urban areas of Rio Grande do Sul State, Brazil, differing in anthropogenic influences and polycyclic aromatic hydrocarbon levels.

    Science.gov (United States)

    Pereira, Tatiana S; Gotor, Gemma N; Beltrami, Laiana S; Nolla, Celia G; Rocha, Jocelita A V; Broto, Francesc P; Comellas, Lluis R; Vargas, Vera M F

    2010-09-30

    Urban areas are both major sources and major targets of air pollution. The atmospheric environment receives diverse chemical substances, including genotoxic agents that may affect human health. The purpose of this study was to evaluate and compare the atmospheric quality in two urban areas in Rio Grande do Sul State (Brazil), under the influence of greater (Site 1) or lesser (Site 2) anthropogenic sources. Polycyclic aromatic hydrocarbons (PAHs) of environmental interest were quantified and the Salmonella/microsome assay was used for the measurement of mutagenicity. Organic compounds extracted from the airborne particulate matter were analyzed by high-performance liquid chromatography (HPLC) to detect PAHs of interest (known or possible carcinogens). The organic extracts were also tested for mutagenic and cytotoxic activity in the Salmonella/microsome assay with strains TA98, TA100, YG1021 and YG1024, with or without S9 activation. At Site 1, benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (IP) were found at higher concentrations and mutagenicity (revertants per μg extract) varied from 1.0±0.25 (TA98, no S9) to 5.2±0.45 (TA98, S9). At Site 2, BghiP and IP were present in larger amounts and the mutagenic responses ranged from 0.6 to 3.7 revertants per μg (both in TA98, S9). The occurrence of BghiP and IP may be related to vehicular emissions. These and the other PAHs studied, as well as the nitro compounds, may contribute to the mutagenicity found in these airborne particles. PMID:20643224

  17. Retrospective analysis of the mutagenicity/genotoxicity data of the cosmetic ingredients present on the Annexes of the Cosmetic EU legislation (2000-12).

    Science.gov (United States)

    Ates, Gamze; Doktorova, Tatyana Y; Pauwels, Marleen; Rogiers, Vera

    2014-03-01

    To evaluate the mutagenicity/genotoxicity of cosmetic ingredients at the regulatory level, usually a battery of three in vitro tests is applied. This battery, designed to be very sensitive, produces a high number of positive results, imposing the need for in vivo follow-up testing to clear the substance under study. In Europe, the use of experimental animals has become impossible for cosmetic ingredients due to the implementation of animal testing and marketing bans. Consequently, the possibility to 'de-risk' substances with positive in vitro results disappear and potentially safe cosmetic substances will be lost for the EU market unless currently used in vitro assays can be adapted or new non-animal mutagenicity/genotoxicity studies become available. Described strategies to improve the specificity of existing in vitro assays include optimisation of the used cell type and cytotoxicity assay and lowering of the applied top concentration. A reduction of the number of tests in the battery from three to two also has been suggested. In this study, the performance of the 'standard' in vitro mutagenicity/genotoxicity testing battery is analysed for a number of cosmetic ingredients. We composed a database with toxicological information on 249 cosmetic ingredients, mainly present on the Annexes of the European cosmetic legislation. Results revealed that the in vitro mutagenicity/genotoxicity tests showed a low specificity for the cosmetic ingredients concerned, comparable to the specificity published for chemicals. Non-confirmed or 'misleading' positive results amounted up to 93% for the in vitro test batteries. The cell type and top concentrations did not have a major impact on the specificity. With respect to cytotoxicity determinations, different end points were used, potentially leading to different testing concentrations, suggesting the need for a consensus in this matter. Overall, the results of this retrospective analysis point to an urgent need of better regulatory

  18. Radiation damages in chemical components of organic scintillator detectors; Danos de radiacao em componentes quimicos de detectores cintiladores organicos

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes Neto, Jose Maria

    2003-07-01

    Samples containing PPO (1%, g/ml), diluted in toluene, they were irradiated in a {sup 60}Co irradiator (6.46 kGy/h) at different doses. The PPO concentration decay bi-exponentially with the dose, generating the degradation products: benzoic acid, benzamide and benzilic alcohol. The liquid scintillator system was not sensitive to the radiation damage until 20 kGy. Otherwise, the pulse height analysis showed that dose among 30 to 40 kGy generate significant loss of quality of the sensor (liquid scintillating) and the light yield was reduced in half with the dose of (34.04 {+-} 0.80) kGy. This value practically was confirmed by the photo peak position analysis that resulted D{sub 1/2} = (31.7 {+-} 1,4) kGy, The transmittance, at 360 nm, of the irradiated solution decreased exponentially. The compartmental model using five compartments (fast decay PPO, slow decay PPO, benzamide, benzoic acid and benzilic alcohol) it was satisfactory to explain the decay of the PPO in its degradation products in function of the dose. The explanation coefficient r{sup 2} = 0.985636 assures that the model was capable to explain 98.6% of the experimental variations. The Target Theory together with the Compartmental Analysis showed that PPO irradiated in toluene solution presents two sensitive molecular diameters both of them larger than the true PPO diameter. >From this analysis it showed that the radiolytic are generated, comparatively, at four toluene molecules diameter far from PPO molecules. For each one PPO-target it was calculated the G parameter (damage/100 eV). For the target expressed by the fast decay the G value was (418.4 {+-} 54.1) damages/100 eV, and for the slow decay target the G value was (54.5 {+-} 8.9) damages/100 eV. The energies involved in the chemical reactions were w (0.239 {+-} 0.031) eV/damage (fast decay) and w = (1 834 {+-} 0.301) eV/damage (slow decay). (author)

  19. Evaluation of the genotoxic and mutagenic potentials of a living nosode compounded with Influenza A virus

    Directory of Open Access Journals (Sweden)

    Alvaro Leitao

    2011-09-01

    Full Text Available Background: The influenza virus has been responsible for contagious respiratory diseases with high mortality rates [1]. Some drugs have been used to treat human influenza. However, these drugs cause many common side effects and induce the appearance of resistant viral strains [2]. The impact caused by the influenza virus has motivated the development of new approaches for the prevention and control of influenza [3]. Therefore, a new homeopathic medicine was developed using, as a starting point, the infectious influenza virus [4]. This belongs to a group called living nosodes [5]. However, its mutagenic and genotoxic potentials, especially when used in low dilutions, has not yet been evaluated and it is important because this biotherapic is prepared from living microorganisms. Different methods can be used to detect mutagenic and genotoxicic effects. Aims: This study aims to evaluate the genotoxic and mutagenic potentials of influenza A living nosode at different homeopathic potencies. Methodology: 1 ml of purified viral suspension was diluted in 9 ml of sterile distilled water. This sample was submitted to 100 mechanical succussions (approximately 3 Hz, using Autic® Brazilian machine, originating the first dilution, named decimal (1x. 1 ml of this solution was diluted in 9 ml of solvent and was submitted to 100 sucussions, generating biotherapic 2x. This procedure was successively repeated, according to Brazilian Homeopathic Pharmacopoeia, to obtain the biotherapic 30x [6]. By the same technique, water vehicle was prepared until 30x potency to be used as control. All samples were prepared in sterile and under aseptic conditions, using laminar flow cabinet, class II, and were stored in the refrigerator (8ºC. The samples 1x, 6x, 12x, 18x, 24x and 30x and water 30x (vehicle control were analysed by: the Inductest, which assesses the ability of physical or chemical agents to promote lysogenic induction as a reflection of damage

  20. Structural and biochemical impact of C8-aryl-guanine adducts within the NarI recognition DNA sequence: influence of aryl ring size on targeted and semi-targeted mutagenicity

    OpenAIRE

    Sproviero, Michael; Verwey, Anne M.R.; Rankin, Katherine M.; Witham, Aaron A.; Soldatov, Dmitriy V.; Richard A. Manderville; Fekry, Mostafa I.; Sturla, Shana J.; Sharma, Purshotam; Wetmore, Stacey D.

    2014-01-01

    Chemical mutagens with an aromatic ring system may be enzymatically transformed to afford aryl radical species that preferentially react at the C8-site of 2′-deoxyguanosine (dG). The resulting carbon-linked C8-aryl-dG adduct possesses altered biophysical and genetic coding properties compared to the precursor nucleoside. Described herein are structural and in vitro mutagenicity studies of a series of fluorescent C8-aryl-dG analogues that differ in aryl ring size and are representative of auth...

  1. Characterization of mutagenic activity in grain-based coffee-substitute blends and instant coffees

    Energy Technology Data Exchange (ETDEWEB)

    Johansson, M.A.E.; Knize, M.G.; Felton, J.S.; Jagerstad, M.

    1994-06-01

    Several grain-based coffee-substitute blends and instant coffees showed a mutagenic response in the Ames/Salmonella test using TA98, YG1024 and YG1O29 with metabolic activation. The beverage powders contained 150 to 500 TA98 and 1150 to 4050 YG1024 revertant colonies/gram, respectively. The mutagenic activity in the beverage powders was shown to be stable to heat and the products varied in resistance to acid nitrite treatment. Characterization of the mutagenic activity, using HPLC-and the Ames test of the collected fractions, showed the coffee-substitutes and instant coffees contain several mutagenic compounds, which are most likely aromatic amines.

  2. Contribution of woodsmoke and motor vehicle emissions to ambient aerosol mutagenicity

    International Nuclear Information System (INIS)

    A multiple linear regression form of receptor modeling has been used to determine the sources of the mutagenicity of fine-particle ambient aerosol samples collected during the winter in a residential area of Albuquerque, NM. Virtually all the mutagenicity (Salmonella typhimurium TA98 + S9) could be accounted for by woodsmoke and motor vehicle emissions. Woodsmoke was found to be the greater contributor to the average ambient concentrations of both extractable organ is and mutagenicity. The mutagenic potency (revertants per microgram) of extractable organics traced to motor vehicles, however, was 3 times greater than that with a woodsmoke origin. The results were confirmed by 14C measurements

  3. Preliminary assessment of mutagenic and anti-mutagenic potential of some aminoalkanolic derivatives of xanthone by use of the Vibrio harveyi assay.

    Science.gov (United States)

    Słoczyńska, Karolina; Waszkielewicz, Anna Maria; Marona, Henryk

    2014-07-01

    The Vibrio harveyi assay was used to evaluate mutagenic and anti-mutagenic effects of four new aminoalkanolic derivatives of xanthone with anticonvulsant activity, to select the potentially safe compounds for further in vivo studies in animal models. The study showed that at a concentration of 40 ng/ml the test compounds were not mutagenic. Additionally, two of the investigated compounds, namely the (R,S)-N-methyl-1-amino-2-propanol derivative of 6-methoxyxanthone (compound III) and the (R)-N-methyl-2-amino-1-butanol derivative of 7-chloroxanthone (compound IV) were strong inhibitors of the mutagenicity induced by 4-nitroquinoline-N-oxide (4-NQO) in V. harveyi strains BB7M and BB7XM. The inhibition percentages for compound IV were 49 (in BB7M) and 69 (in BB7XM), whereas for compound III these percentages were 47 (in BB7M) and 42 (in BB7XM), respectively. The present study demonstrates that four bioactive derivatives of xanthone display no mutagenic activity in the V. harveyi assay. In addition, compounds III and IV demonstrated considerable anti-mutagenic activity in this test. Based on the results obtained here, these compounds could be selected for further studies in animal models, while compounds III and IV should be tested further for their anti-mutagenic properties. PMID:24769486

  4. Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut

    International Nuclear Information System (INIS)

    The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm−3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10–50 Gy) or treated with 1–5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm−3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm−3) and 0.5 mg dm−3 2,4-D for maturation. The well-developed embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm−3 BAP and 0.25 mg dm−3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape. (author)

  5. Reproducibility of microbial mutagenicity assays. I. Tests with Salmonella typhimurium and Escherichia coli using a standardized protocol

    International Nuclear Information System (INIS)

    The Salmonella/microsome test developed by Ames and his coworkers has been widely used in the evaluation of chemicals for genotoxic potential. Although the value of this assay is well recognized, there have been no comprehensive studies on the interlaboratory reproducibility of the method using a standardized protocol. A program was therefore initiated to compare the results obtained in four laboratories from testing a series of coded mutagens and nonmutagens using a standardized protocol. Additional objectives of this study were to compare male Fisher 344 rat, B6C3F1 mouse, and Syrian hamster liver S-9 preparations for the activation of chemicals; to compare Aroclor 1254-induced liver S-9 from all three species with the corresponding non-induced liver S-9's; and to compare the response of Escherichia coli WP-2 uvrA with the Salmonella typhimurium tester strains recommended by Ames. Since a primary use of in vitro microbial mutagenesis tests is the identification of potential carcinogens by their mutagenicity, the authors decided to compare the animal species and strains used by the National Cancer Institute/National Toxicology Program (NCI/NTP) for animal carcinogenicity studies

  6. BIOMETRICAL, BACTERIOLOGICAL AND PHYSICAL-CHEMICAL PARAMETERS OF THE CURIMATÃ-PACU (Prochilodus marggravii) REARED CONSORTIATED WITH SWINE PARÂMETROS BIOMÉTRICOS, BACTERIOLÓGICOS E FÍSICO-QUÍMICOS DO CURIMATÃ-PACU (Prochilodus marggravii) CRIADO EM CONSÓRCIO COM SUÍNOS

    OpenAIRE

    Iolanda Aparecida Nunes; Edmar Soares Nicolau; Albenones José de Mesquita; paulo César Silva; Gerson José de Oliveira

    2007-01-01

    Samples of curimatã-pacu (<em>Prochilodus> <em>marggravii>) reared consortiated with swine and killed after 368 days of observations studied regarding to the biometrical, bacteriological and physical-chemical parameters. It was observed that the fishes showed an average of live weight of about 606.50g, a medium of pattern length of 27.30 cm, a carcass gain of 67.25%, an average of 18...

  7. Sistemas agroflorestais e seus efeitos sobre os atributos químicos em Argissolo Vermelho-Amarelo do Cerrado piauiense Agroforestry systems and its effects on chemical attributes of an Ultisol in the 'Cerrado' of Piaui state, Brazil

    Directory of Open Access Journals (Sweden)

    Bruna de F. Iwata

    2012-07-01

    Full Text Available Objetivou-se, com este trabalho, quantificar os efeitos dos Sistemas Agroflorestais com diferentes tempos de adoção e a agricultura de corte e queima sobre os atributos químicos de um Argissolo Vermelho-Amarelo em áreas de cerrado do estado do Piauí. Em duas épocas climáticas foram estudados quatro sistemas: sistemas agroflorestais com seis e treze anos de adoção, agricultura de corte e queima e uma floresta nativa do cerrado. As amostras de solo foram coletadas em quatro profundidades para determinação dos atributos químicos. No solo sob os Sistemas Agroflorestais foram observados, em todas as camadas avaliadas, redução dos teores de Al3+ e H++Al3+ e aumento do pH, dos teores dos nutrientes e do carbono orgânico total em relação aos demais sistemas. No solo sob agricultura de corte e queima, verificou-se que apenas nas camadas superficiais a cinza depositada sobre o solo promoveu aumento dos nutrientes, exceto para o P, que foi maior em todas as camadas. Nos demais sistemas houve diminuição dos teores dos nutrientes no período chuvoso. Os sistemas agroflorestais promoveram aumento dos teores de nutrientes do solo e garantiram melhoria da qualidade química do solo em áreas de cerrado no estado do Piauí.This study aimed to quantify the effects of agroforestry systems (AFS, with different periods of adoption, and slash and burn agriculture (SB on the chemical attributes of an Ultisol in the 'Cerrado' of Piaui State, Brazil. In two distinct climatic seasons (dry and rainy four systems were studied: AFS with six (AFS6 and thirteen years (AFS13 of adoption, an area under SB and a native forest (NF, as a reference. In the AFS, in all depths, higher reduction in the Al3+ and H+ + Al3+ contents were observed as well as an increase of pH value and contents of nutrients (N, P, Ca, Mg and K and organic carbon in soil. In the soil under SB, only in superficial layers, the ash deposited on the soil caused an increase in contents of

  8. Evaluation of Mutagenicity of Three Eugenol-Containing Materials

    OpenAIRE

    Narlan Sumawinata

    2015-01-01

    Eugenol containing materials are still widely used, both by the lay people or by the dentist. Professionally it is used to relieve dental pain by placing it in the cavity, or as a mixture for temporary filling, temporary cementation, and root canal sealer. Eugenol, however, is also known to be toxic to the tissue, and aflatoxin, a substance known to be mutagenic, has been found in material labeled as clove oil. The purpose of this study was to investigate the safety of these materials, in par...

  9. Cloning of Salmonella typhimurium DNA encoding mutagenic DNA repair.

    OpenAIRE

    Thomas, S M; Sedgwick, S G

    1989-01-01

    Mutagenic DNA repair in Escherichia coli is encoded by the umuDC operon. Salmonella typhimurium DNA which has homology with E. coli umuC and is able to complement E. coli umuC122::Tn5 and umuC36 mutations has been cloned. Complementation of umuD44 mutants and hybridization with E. coli umuD also occurred, but these activities were much weaker than with umuC. Restriction enzyme mapping indicated that the composition of the cloned fragment is different from the E. coli umuDC operon. Therefore, ...

  10. A nonparametric approach to the statistical analysis of mutagenicity data.

    Science.gov (United States)

    Wahrendorf, J; Mahon, G A; Schumacher, M

    1985-01-01

    A nonparametric statistical method for analysing count data from mutagenicity tests is proposed. This method does not make any stringent assumptions on the variation of the data. It requires only one ranking of the observations and the calculation of a resulting test statistic. The significance of this statistic can be assessed by approximation to the standard normal distribution or tables of critical values calculated by the authors. The method also includes a descriptive component through a series of K-1 estimates of a trend in a K group experiment. Several examples are discussed to illustrate the proposed method. PMID:3974612

  11. Cholesterol epoxide is a direct-acting mutagen.

    OpenAIRE

    A. Sevanian; Peterson, A R

    1984-01-01

    A 24-hr treatment of V79 Chinese hamster lung fibroblasts with 12.4 microM cholesterol 5 alpha, 6 alpha-epoxide induced 8-azaguanine-resistant mutants at frequencies 4.6- to 11.8-fold higher than the spontaneous mutation rate. We show that cholesterol epoxide, which is produced by in vivo cholesterol oxidation, is a weak direct-acting mutagen. Cholesterol epoxide was found to be accumulated by cells and transformed to cholestane-3 beta, 5 alpha, 6 beta-triol, which was more toxic and a more p...

  12. How to assess the mutagenic potential of cosmetic products without animal tests?

    Science.gov (United States)

    Speit, Günter

    2009-08-01

    Animal experiments (in vivo tests) currently play a key role in genotoxicity testing. Results from in vivo tests are, in many cases, decisive for the assessment of a mutagenic potential of a test compound. The Seventh Amendment to the European Cosmetics Directive will, however, ban the European marketing of cosmetic/personal care products that contain ingredients that have been tested in animal experiments. If genotoxicity testing is solely based on the currently established in vitro tests, the attrition rate for chemicals used in cosmetic products will greatly increase due to irrelevant positive in vitro test results. There is urgent need for new and/or improved in vitro genotoxicity tests and for modified test strategies. Test strategies should consider all available information on chemistry of the test substance/the chemical class (e.g. SAR, metabolic activation and dermal adsorption). Test protocols for in vitro genotoxicity tests should be sensitive and robust enough to ensure that negative results can be accepted with confidence. It should be excluded that positive in vitro test results are due to high cytotoxicity or secondary genotoxic effects which may be thresholded and/or only occur under in vitro test conditions. Consequently, further research is needed to establish the nature of thresholds in in vitro assays and to determine the potential for incorporation of mode of action data into future risk assessments. New/improved tests have to be established and validated, considering the use of (metabolically competent) primary (skin) cells, 3D skin models and cells with defined capacity for metabolic activation (e.g. genetically engineered cell lines). The sensitivity and specificity of new and improved genotoxicity tests has to be determined by testing a battery of genotoxic and non-genotoxic chemicals. New or adapted international guidelines will be needed for these tests. The establishment of such a new genotoxicity testing strategy will take time and the

  13. Mutagenicity assessment of aerosols in emissions from wood combustion in Portugal

    International Nuclear Information System (INIS)

    Polycyclic aromatic hydrocarbon (PAH) extracts of fine particles (PM2.5) collected from combustion of seven wood species and briquettes were tested for mutagenic activities using Ames test with Salmonella typhimurium TA98 and TA100. The woods were Pinus pinaster (maritime pine), Eucalyptus globulus (eucalypt), Quercus suber (cork oak), Acacia longifolia (golden wattle), Quercus faginea (Portuguese oak), Olea europea (olive), and Quercus ilex rotundifolia (Holm oak). Burning experiments were done using woodstove and fireplace, hot start and cold start conditions. A mutagenic response was recorded for all species except golden wattle, maritime pine, and briquettes. The mutagenic extracts were not correlated with high emission factors of carcinogenic PAHs. These extracts were obtained both from two burning appliances and start-up conditions. However, fireplace seemed to favour the occurrence of mutagenic emissions. The negative result recorded for golden wattle was interesting, in an ecological point of view, since after confirmation, this invasive species, can be recommended for domestic use. - Highlights: ► Both woodstove and fireplace, either with a cold or hot start, produce emissions with mutagenic potential. ► The high level of carcinogenic PAHs in combustion emissions was not correlated with mutagenicity. ► The golden wattle, an invasive species, produced no mutagenic emissions. - Wood smoke from fireplace burning of dominant forest species displayed strong mutagenic activity without a significant correlation with carcinogenic PAHs emission factors.

  14. Gamma radiation/H2O2 treatment of a nonylphenol ethoxylates: Degradation, cytotoxicity, and mutagenicity evaluation.

    Science.gov (United States)

    Iqbal, Munawar; Bhatti, Ijaz Ahmad

    2015-12-15

    Gamma radiation/H2O2 treatment of nonylphenol polyethoxylates (NPEO) was performed and treatment effect was evaluated on the basis of degradation, chemical oxygen demand (COD) and total organic carbon (TOC), and toxicity reduction efficiencies. The radiolytic by-products were determined by Fourier Transform Infrared Spectroscopy (FTIR), High-Performance Liquid Chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS) techniques. Low mass carboxylic acids, aldehyde, ketone, and acetic acid were identified as the by-products of the NPEO degradation. NPEO sample irradiated to the absorbed dose of 15 kGy/4.58% H2O2 showed more than 90% degradation. Allium cepa (A. cepa), brine shrimp, heamolytic tests were used for cytotoxicity study, while mutagenicity was evaluated through Ames test (TA98 and TA100 strains) of treated and un-treated NPEO. The reductions in COD and TOC were greater than 70% and 50%, respectively. Gamma radiation/H2O2 treatment revealed a considerable reduction in cytotoxicity and mutagenicity. A. cepa, heamolytic and shrimp assays showed cytotoxicity reduction up to 68.65%, 77%, and 94%, respectively. The mutagenicity reduced up to 62%, 74%, and 79% (TA98) and 68%, 78%, and 82% (TA100), respectively of NPEO-6, NPEO-9, and NPEO-30 irradiated to the absorbed dose of 15 kGy/4.58% H2O2. NPEO-6 detoxified more efficiently versus NPEO-9 and NPEO-30 and results showed that Gamma radiation/H2O2 treatment has the potential to mineralize and detoxify NPEO. PMID:26143198

  15. Mutagenic effects of β-rays on rice (oryza sativa L.)

    International Nuclear Information System (INIS)

    The mutagenic effects of 14C and some other mutagenic factors were compared, and the relationships between mutagenic effects of 14C and treated stages, doses and methods were studied with different rice varieties as test materials. The mutation rates of heading date and plant height were observed in M2. The results showed that the mutagenic effects of 14C were better than those of other mutagenic factors tested. It is most effective for inducing early-maturing mutation to treat plants with the doses of 333 x 104 Bq/plant at the stage of pollen mother cell formation; but for dwarf mutation , they were treated with 74 x 104 Bq/plant at the stage of pistil and stamen formation to pollen mother cell formation. As a best treating method, Na214CO3 solution was injected to plant bases

  16. Genetically Modified Vibrio harveyi Strains as Potential Bioindicators of Mutagenic Pollution of Marine Environments

    Science.gov (United States)

    Czyż, Agata; Jasiecki, Jacek; Bogdan, Adam; Szpilewska, Hanna; Węgrzyn, Grzegorz

    2000-01-01

    For biodetection of mutagenic pollution of marine environments, an organism naturally occurring in these habitats should be used. We found that marine bacterium Vibrio harveyi may be an appropriate bioindicator of mutagenic pollution. For positive selection of mutants, we developed a simple method for isolation of V. harveyi mutants resistant to neomycin. We constructed genetically modified V. harveyi strains that produce significantly more neomycin-resistant mutants upon treatment with low concentrations of mutagens than the wild-type counterpart. The sensitivity of the mutagenicity test with the V. harveyi strains is at least comparable to (if not higher than) that of the commonly used Ames test, which uses Salmonella enterica serovar Typhimurium strains. Therefore, we consider that the V. harveyi strains described in this report could be used as potential bioindicators of mutagenic pollution of marine environments. PMID:10653723

  17. Genetically modified Vibrio harveyi strains as potential bioindicators of mutagenic pollution of marine environments

    Energy Technology Data Exchange (ETDEWEB)

    Czyz, A.; Jasiecki, J.; Bogdan, A.; Szpilewska, H.; Wegrzyn, G.

    2000-02-01

    For biodetection of mutagenic pollution of marine environments, an organism naturally occurring in these habitats should be used. The authors found that marine bacterium Vibrio harveyi may be an appropriate bioindicator of mutagenic pollution. For positive selection of mutants, they developed a simple method for isolation of V. harveyi mutants resistant to neomycin. The authors constructed genetically modified V. harveyi strains that produce significantly more neomycin-resistant mutants upon treatment with low concentrations of mutagens than the wild-type counterpart. The sensitivity of the mutagenicity test with the V. harveyi strains is at least comparable to (if not higher than) that of the commonly used Ames test, which uses Salmonella enterica serovar Typhimurium strains. Therefore, the authors consider that the V. harveyi strains described in this report could be used as potential bioindicators of mutagenic pollution of marine environments.

  18. Controle químico de Typha subulata em dois estádios de desenvolvimento Chemical control of Typha subulata in two growth stages

    Directory of Open Access Journals (Sweden)

    J.R.V. Silva

    2004-09-01

    Full Text Available O objetivo deste trabalho foi avaliar o controle químico de plantas de taboa em dois estádios fenológicos de desenvolvimento, ou seja, no estádio de pleno desenvolvimento vegetativo de 0,50 a 0,70 m e no estádio de florescimento. Os herbicidas e as doses utilizadas foram: imazapyr a 250, 500 e 750 g ha-1 com 0,5% de Aterbane; imazapyr a 250, 500 e 750 g ha-1 com 0,01% de Silwet; glyphosate a 3.360 e 4.320 g ha-1 com 0,5% de Aterbane; glyphosate a 3.360 e 4.320 g ha-1 com 0,01% de Silwet, além de uma testemunha sem aplicação de herbicidas. Os tratamentos foram instalados em delineamento experimental inteiramente casualizado, com quatro repetições. Os herbicidas foram aplicados com um pulverizador costal, munido de barra com duas pontas de jato plano XR Teejet 8002 S, a pressão constante de CO2 a 220 KPa, com consumo de calda de 200 L ha-1. As plantas de taboa foram mais sensíveis aos herbicidas quando no estádio de pleno desenvolvimento vegetativo de crescimento que no estádio de pleno florescimento, observando controle aceitável tanto com imazapyr como com glyphosate, exceto a dose de 250 g ha-1 de imazapyr com 0,5% de Aterbane. Neste mesmo estádio foi observado que o surfatante Aterbane foi menos efetivo que o Silwet, quando adicionado à menor dose do herbicida imazapyr.The aim of this research was to evaluate cattail chemical control in two growth stages: full vegetative development (0.50-0.70 m and full flowering. The herbicide rates applied were: imazapyr at 250, 500 and 750 g ha-1 plus Aterbane at 0.5%; imazapyr at 250, 500 and 750 g ha-1 plus 0.01% of Silwet; glyphosate at 3,360 and 4,320 g ha-1 plus Aterbane at 0.5%; glyphosate at 3,360 and 4,320 g ha-1 plus 0.01% of Silwet, and a check without herbicide treatment. The experiment was arranged in a completely randomized design, with four replications. Treatments were sprayed using a CO2 backpack sprayer at 220 Kpa, with two XR Teejet 8002S nozzle tips, with a spray volume

  19. Evaluation of in vivo mutagenicity of hydroquinone in Muta™ mice.

    Science.gov (United States)

    Matsumoto, Mariko; Masumori, Shoji; Hirata-Koizumi, Mutsuko; Ono, Atsushi; Honma, Masamitsu; Yokoyama, Kazuhito; Hirose, Akihiko

    2014-12-01

    Hydroquinone (HQ) is used in skin bleaching agents, hair dyes, and finger nail treatments. Many skin-lightening cosmetics that contain HQ are currently marketed in Japan. Concerns have been expressed regarding health risks to the general population because the carcinogenicity of HQ was previously suggested in animal studies. HQ induced hepatocellular adenomas and forestomach hyperplasias in mice and renal tubular cell adenomas in male rats. In the present study, the lacZ transgenic mutation assay was conducted according to OECD test guideline 488 to determine whether mutagenic mechanisms were involved in HQ-induced carcinogenesis. Male Muta™ mice were repeatedly administered HQ orally at dosages of 0, 25, 50, 100, or 200mg/kg bw/day for 28 days. Body weight gain was decreased in all treatment groups. No significant differences were observed in mutant frequencies in the liver, stomach, lung, or kidney between HQ-treated mice and the concurrent negative controls, whereas the significant induction of mutations was noted in the positive control, N-ethyl-N-nitrosourea. These results suggest that a mutagenic mechanism is not responsible for HQ-induced carcinogenesis. PMID:25435360

  20. Mutagenicity of diesel exhaust soot dispersed in phospholipid surfactants

    Energy Technology Data Exchange (ETDEWEB)

    Wallace, W.; Keane, M.; Xing, S.; Harrison, J.; Gautam, M.; Ong, T.

    1994-06-01

    Organics extractable from respirable diesel exhaust soot particles by organic solvents have been known for some time to be direct acting frameshift mutagens in the Ames Salmonella typhimurium histidine reversion assay. Upon deposition in a pulmonary alveolus or respiratory bronchiole, respirable diesel soot particles will contact first the hypophase which is coated by and laden with surfactants. To model interactions of soot and pulmonary surfactant, the authors dispersed soots in vitro in the primary phospholipid pulmonary surfactant dipalmitoyl glycerophosphorylcholine (lecithin) (DPL) in physiological saline. They have shown that diesel soots dispersed in lecithin surfactant can express mutagenic activity, in the Ames assay system using S. typhimurium TA98, comparable to that expressed by equal amounts of soot extracted by dichloromethane/dimethylsulfoxide (DCM/DMSO). Here the authors report additional data on the same system using additional exhaust soots and also using two other phospholipids, dipalmitoyl glycerophosphoryl ethanolamine (DPPE), and dipalmitoyl phosphatidic acid (DPPA), with different ionic character hydrophilic moieties. A preliminary study of the surfactant dispersed soot in an eucaryotic cell test system also is reported.

  1. Toxicity and mutagenic activity of some selected Nigerian plants.

    Science.gov (United States)

    Sowemimo, A A; Fakoya, F A; Awopetu, I; Omobuwajo, O R; Adesanya, S A

    2007-09-25

    The toxicity and mutagenic potential of most African plants implicated in the management of cancer have not been investigated. The ethanolic extracts of selected Nigerian plants were subsequently studied using the brine shrimp lethality tests, inhibition of telomerase activity and induction of chromosomal aberrations in vivo in rat lymphocytes. Morinda lucida root bark, Nymphaea lotus whole plant and Garcinia kola root were active in the three test systems. Bryophyllum calycinum whole plant, Annona senegalensis root, Hymenocardia acida stem bark, Erythrophleum suaveolens leaves and Spondiathus preussii stem bark were toxic to brine shrimps and caused chromosomal damage in rat lymphocytes. Ficus exasperata leaves, Chrysophyllum albidum root bark and Hibiscus sabdariffa leaves were non-toxic to all the three test systems. Chenopodium ambrosioides whole plant was non-toxic to brine shrimps and rat lymphocyte chromosomes but showed inhibition in the conventional telomerase assay indicating a possible selectivity for human chromosomes. The result justified the use of the first eight plants and Chenopodium ambrosioides in the management of cancer in south west Nigeria although they appear to be non-selective and their mode of action may be different from plant to plant. All these plants except Chenopodium ambrosioides are also mutagenic and cytotoxic. PMID:17707603

  2. Molecular basis for the mutagenic and lethal effects of ultraviolet irradiation. Research accomplishments (1968 to present)

    International Nuclear Information System (INIS)

    Earlier work on the chemical basis of mutagenesis led to certain chemical generalities sufficient to explain how certain mutagens such as uv light and hydroxylamine functioned in information transfer systems (replicative, transcriptive and translational). When such modifications were applied to biologically active DNA in a controlled manner biological expression was non-stoichiometric because much of the damage was removed from the DNA by repair systems. Our efforts were then directed to these systems which led to: (1) the isolation, purification and characterization of endonucleases responsible for the first and controlling step in DNA repair - referred to as incision in both M. luteus and E. coli. The biological role of these enzymes was inferred in appropriate mutants; (2) the isolation, purification and characterization of exonucleases responsible for the removal or excision of damaged nucleotides in M. luteus and human placental trophoblasts; (3) the repair of uv damaged biologically active transforming and transfecting DNAs by purified endonucleases, exonucleases, DNA polymerase I and polynucleotide ligase from M. luteus and E. coli; (4) the characterization of the dual gene control for incision phenomenon in M. luteus and E. coli; and (5) isolation, purification and characterization of repair enzymes from human placenta

  3. Efeito do uso do xisto em características químicas do solo e nutrição do tomateiro Effect of the use of schist on chemical characteristics of soil and on tomato nutrition

    Directory of Open Access Journals (Sweden)

    Hamilton Seron Pereira

    2004-06-01

    Full Text Available Um dos principais problemas ambientais que ocorre com o processo de exploração do xisto é a produção de quantidades maciças de xisto retortado de alta alcalinidade. Este resíduo, depositado em larga escala, libera elementos tóxicos no ambiente, poluindo o solo, a água de superfície e até o ar atmosférico. Avaliou-se o efeito do xisto nas características químicas do solo e na nutrição do tomateiro em dois experimentos conduzidos em Argissolos Vermelho Amarelo, em ambiente protegido. Foram aplicadas e incorporadas ao solo as doses de 0; 3; 6; 9 e 12 t ha-1 de xisto. O delineamento utilizado foi em blocos ao acaso com quatro repetições. A adição de xisto promoveu aumento significativo nos teores de enxofre e silício no solo e nas folhas do tomateiro. Não foram verificadas alterações no valor do pH do solo nem nos teores de cátions trocáveis, mas, observou-se aumento na disponibilidade de fósforo. Não se verificou dano ao ambiente, por contaminações químicas de metais pesados, nem diminuição na produtividade do tomate em função da aplicação dos tratamentos. O xisto se mostrou apto para ser usado na agricultura, nas doses utilizadas.Among the main environmental problems due to the use of schist is a massive production of highly alkaline schist processing waste. This residue, when deposited in great amounts liberates toxic elements in the atmosphere, polluting the soil, the surface water and the atmospheric air. The effect of the use of schist in agriculture was evaluated on the soil chemical attributes and tomato crop nutrition. Two experiments were performed on Typic Eutrustults under greenhouse conditions. Schist rates of 0; 3; 6; 9 and 12 t ha-1 were applied and incorporated in the soil. A randomized block design with four replications was used. Schist use promoted a significant sulfur and silicon content increase in the soil as well as in the tomato leaves; no soil pH alteration was verified neither the

  4. Variabilidade espacial de atributos químicos em um Argissolo sob pastagem = Spatial variability of chemical attributes in an Ultisol under pasture

    Directory of Open Access Journals (Sweden)

    Gustavo Soares de Souza

    2008-10-01

    Full Text Available Estudos mostram a importância de se conhecer a variabilidade dos atributos do solo para o manejo mais eficiente. O objetivo deste trabalho foi analisar a variabilidade espacial dos atributos químicos de um Argissolo Vermelho-Amarelo, cultivado com pastagem de Brachiaria decumbens, em Alegre – Espírito Santo. Amostras de solo foram coletadas na profundidade de 0-0,2 m, nos pontos de cruzamento de uma malha com intervalos regulares de 10 m, perfazendo o total de 64 pontos. Os dados foram submetidos à analise estatística descritiva, geoestatística e interpolação por krigagem ordinária. Os valores dos coeficientes de variação apresentaram-se baixos para pH, altos para Al e m% e médios para os demais atributos. Os atributos pH, P, H+Al e m% apresentaram forte dependência espacial e os demais, moderada dependência. Todos os atributos estudados apresentaramestrutura de dependência espacial, o que permitiu o seu mapeamento com técnicas de geoestatísticas.Studies show the importance of knowing the variability of soil attributes for a more efficient management. This work was carried out to evaluate the spatial variability of the chemical attributes of an Ultisol, cultivated with Brachiaria decumbens pasture in Alegre - ES. Soil samples were collected at a depth of 00-0.2 m, at the crossing points of a regular grid with 10 m-intervals, comprising a total of 64 points. Data were submitted to descriptive statistics, geostatistics and kriging interpolation analysis. The coefficient of variation was low for pH, high for Al and m%, and medium the other attributes. The attributes pH, P, H+Al and m% presented strong dependence, and the other moderate dependence. The attributes presented a spatial dependence structure, allowing their mapping by geostatistics techniques.

  5. Anti mutagenesis of chemical modulators against damage induced by reactor thermal neutrons; Antimutagenesis de moduladores quimicos contra el dano inducido por neutrones termicos de reactor

    Energy Technology Data Exchange (ETDEWEB)

    Zambrano A, F.; Guzman R, J.; Garcia B, A.; Paredes G, L.; Delfin L, A. [Instituto Nacional de Investigaciones Nucleares, Departamentos de Materiales Radiactivos, de Biologia, del Reactor y Gerencia de Aplicaciones Nucleares en la Salud, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    The mutations are changes in the genetic information whether for spontaneous form or induced by the exposure of the genetic material to certain agents, called mutagens: chemical or physical (diverse types of radiations). As well as exist a great variety of mutagens and pro mutagens (these last are agents which transform themselves in mutagens after the metabolic activation). Also several chemical compounds exist which are called antimutagens because they reduce the mutagens effect. The C vitamin or ascorbic acid (A A) presents antimutagenic and anti carcinogenic properties. On the other hand a sodium/copper salt derived from chlorophyll belonging to the porphyrin group (C L) contains a chelated metal ion in the center of molecule. It is also an antioxidant, antimutagenic and anti carcinogenic compound, it is called chlorophyllin. The objective of this work is to establish if the A A or the C L will reduce the damages induced by thermal and fast reactor neutrons. (Author)

  6. On the need to assess cancer risk in populations environmentally and occupationally exposed to virus and chemical agents in developing countries Sobre a necessidade de avaliação dos riscos de câncer em populações dos países em desenvolvimento expostas ambiental e ocupacionalmente a vírus e agentes químicos

    Directory of Open Access Journals (Sweden)

    Guilherme Franco Netto

    1998-01-01

    Full Text Available Evidence exists that exposure to poultry oncogenic viruses may produce elevated cancer mortality in human populations, particularly excesses of cancer of lung and excesses of cancer of lymphopoietic tissues. To date, this potential risk is unknown in populations from the developing countries. This paper suggests the need to assess cancer risk in populations of developing countries with reported environmental exposure to chicken meat products and eggs; the need to assess risk of cancer in populations inoculated with vaccines from infected chicken embryos; and the need to assess risk of cancer in occupational populations highly exposed to poultry oncogenic viruses, and with potential concurrent exposure to chemical agents known or suspected to be carcinogens.Existe evidência de que exposição a vírus oncogênicos de aves possa produzir elevada mortalidade de câncer em populações humanas, havendo particularmente excesso de câncer de pulmão e dos sistemas linfáticos e hemopoiéticos. Até o momento, esse risco potencial é desconhecido em populações dos países em desenvolvimento. Este manuscrito sugere a necessidade de avaliar o risco de câncer em populações dos países em desenvolvimento com sabida exposição a produtos avícolas e ovos; a necessidade de avaliar o risco de câncer em populações que foram inoculadas com vacinas desenvolvidas em embriões de aves contaminadas e a necessidade de avaliar o risco de câncer em populações de trabalhadores com grande exposição a vírus oncogênico de aves, e com potencial exposição simultânea a agentes químicos que são reconhecidos ou suspeitos carcinógenos.

  7. Sensitivity of watermelon variety Bojura to mutant agents 60Co and EMS

    OpenAIRE

    Nikolay Velkov; Nasya Tomlekova; Fatma Sarsu

    2016-01-01

    A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with 60Co gamma rays (80, 100, 200, 250, 350 and 450 Gy) and swollen seeds with water for 24 hours were treated with ethyl methanesulfonate (EMS) at a concentration of 2%. Dominant mutations were not observed in the M1 generation. Morphological changes in 14 of 1395 M2 plants were observed. Phenotypic variation...

  8. Gamma ray and ems induced genetic variability for quantitative traits in urdbean (Vigna mungo l. Hepper)

    International Nuclear Information System (INIS)

    Range, mean and coefficient of variation for different characters in two cultivars, namely, PDUI and T9 of urdbean suggested that the mutagenic treatments had wider values than control. Positive and negative shifts in mean were observed for all the quantitative traits in both the cultivars. All the mutagenic treatments influenced the mean, range and CV values independently in both the varieties. However, the combination of varying doses of gamma rays with EMS (0.02 M) was found effective for causing induced genetic variability in urdbean cultivars

  9. Evaluation of mutagenic and antimutagenic properties of new derivatives of pyrrolidine-2,5-dione with anti-epileptic activity, by use of the Vibrio harveyi mutagenicity test.

    Science.gov (United States)

    Pękala, Elżbieta; Liana, Piotr; Kubowicz, Paulina; Powroźnik, Beata; Obniska, Jolanta; Chlebek, Iwona; Węgrzyn, Alicja; Węgrzyn, Grzegorz

    2013-12-12

    The Vibrio harveyi test was used to evaluate mutagenic and antimutagenic properties of nineteen new derivatives of pyrrolidine-2,5-dione (compounds 1-19) with antiepileptic activity. Four V. harveyi strains were used: BB7 (wild type) and the genetically modified strains BB7M, BB7X and BB7XM (i.e. strains with additional mucA and mucB genes, UV hypersensitivity, and UV hypersensitivity with plasmid pAB91273, respectively). None of the derivatives of 2-ethyl-2-methylsuccinic acid (compounds 1-7) had mutagenic activity against the tester strains of V. harveyi, but this set had strong or moderate antimutagenic activity against 4-nitroquinoline-N-oxide (NQNO) in the tester strains BB7, BB7X, and BB7M. This antimutagenic activity ranged from 51% to 67%, through 51-66% to 71-83% for V. harveyi BB7, BB7X and BB7M strains, respectively. Mutagenic activities in the group of 2,2-diphenyl-succinic acid derivatives (compounds 8-19) were variable and depended on the tester strain used. Compounds 8-19 were devoid of mutagenic properties against BB7 (wild-type strain). Among this group only compound 9, with the fluorine substituent in position 2 of the aromatic system, was devoid of mutagenic potential against all tester strains. The compounds in this group (8-19) demonstrated strong antimutagenic activity only against strain BB7 (inhibition ranging from 51% to 71%). We conclude that there are various mutagenic and antimutagenic activities of derivatives of pyrrolidine-2,5-dione. Moreover, our studies have proven that the V. harveyi test can be applied for primary mutagenicity and antimutagenicity assessment of these new compounds. PMID:24060509

  10. Terpene Glycosides from the Roots of <em>Sanguisorba officinalisem> L. and Their Hemostatic Activities

    OpenAIRE

    Wei Sun; Zi-Long Zhang; Xin Liu; Shuang Zhang; Lu He; Zhe Wang; Guang-Shu Wang

    2012-01-01

    Guided by a hemostasis bioassay, seven terpene glycosides were isolated from the roots of <em>Sanguisorba officinalisem> L. by silica gel column chromatography and preparative HPLC. On the grounds of chemical and spectroscopic methods, their structures were identified as citronellol-1-<em>O>-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (1), geraniol-1-<em>O>-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (

  11. Samplings of urban particulate matter for mutagenicity assays

    International Nuclear Information System (INIS)

    In the frame of a specific program relating to the evaluation of mutagenic activity of urban particulate matter, an experimental arrangement has been developed to sample aerosuspended particles from the external environment carried indoor by means of a fan. Instrumentation was placed directly in the air flow to minimize particle losses, and consisted of total filter, collecting particles without any size separation; cascade impactor, fractioning urban particulate to obtain separate samples for analyses; an optical device, for real time monitoring of aerosol concentration, temperature and relative humidity sensors. Some of the samples obtained were analysed to investigate: particle morphology, aerosol granulometric distributions, effect of relative humidity on collected particulate, amount of ponderal mass compared with real time optical determinations. The results obtained are reported here, together with some considerations about carbonaceous particles, in urban areas mainly originated from diesel exhausts, their degree of agglomeration and role to vehiculate substances into the human respiratory

  12. Mutagenic effect of radiations of various LET on Salmonella cells

    International Nuclear Information System (INIS)

    Regularities of mutagenic effect of heavy charged particles of helium, deuterium ions and protons on various strains of Salmonella typhimurium were studied. Linear dose dependences of mutation frequency in the range of low doses were revealed. A conclusion was made that mutation process at low dose irradiation is determined in various teststrains of Salmonella typhimurium by certain premutation injuries. Quite a different picture is observed in mutation process in case of high dose irradiation where effect of inducible SOS-repair is distinctly manifested. Not only spectra of primary DNA injuries but probability of their fixation into mutation can change with the increase of LET. If fixation probability doesn't change with LET increase for replicative mutagenesis which make basis contribution to linear component of dose dependence of mutation frequency the probability of fixation is increased for reparative mutagenesis. It accounts for increase of values of relative genetic efficiency with LET increase. 7 refs.; 6 figs.; 1 tab

  13. BIOMETRICAL, BACTERIOLOGICAL AND PHYSICAL-CHEMICAL PARAMETERS OF THE CURIMATÃ-PACU (Prochilodus marggravii REARED CONSORTIATED WITH SWINE PARÂMETROS BIOMÉTRICOS, BACTERIOLÓGICOS E FÍSICO-QUÍMICOS DO CURIMATÃ-PACU (Prochilodus marggravii CRIADO EM CONSÓRCIO COM SUÍNOS

    Directory of Open Access Journals (Sweden)

    Iolanda Aparecida Nunes

    2007-09-01

    Full Text Available

    Samples of curimatã-pacu (<em>Prochilodus> <em>marggravii> reared consortiated with swine and killed after 368 days of observations studied regarding to the biometrical, bacteriological and physical-chemical parameters. It was observed that the fishes showed an average of live weight of about 606.50g, a medium of pattern length of 27.30 cm, a carcass gain of 67.25%, an average of 18.38% of proteins, pH medium of 6.24 and excellent bacteriological quality.

    Exemplares de curimatã-pacu (<em>Prochilodus> <em>marggravii>, criados em consórcio com suínos e abatidos aos 368 dias, foram estudados quanto a parâmetros biométricos, físico-químicos e bacteriológicos. Observou-se que os peixes apresentaram média de peso vivo de 606,50 g, média de comprimento padrão de 27,30 cm, rendimento médio de carcaça de 67,25%, média de proteína de 18,38%, média de pH de 6,24 e excelente qualidade bacteriológica.

  14. Controle de estoque de materiais com diferentes padrões de demanda: estudo de caso em uma indústria química Inventory control of items with different demand patterns: a case study in the chemical industry

    Directory of Open Access Journals (Sweden)

    Antônio Marcos dos Santos

    2006-05-01

    Full Text Available O desafio do gestor de estoques é saber quando e quanto ressuprir de cada material e quanto deve manter em estoque de segurança. Com o crescente número de itens com diferentes padrões de demanda e características específicas, a complexidade na administração de materiais aumenta devido à necessidade de controle diferenciado. Este trabalho, por meio de um estudo de caso em uma empresa química, propõe um método de classificação dos materiais em famílias afins com a adoção de políticas distintas de ressuprimento e estoques de segurança, com o objetivo de garantir o balanceamento dos estoques e atender aos níveis de serviço requeridos à produção.The main challenge of inventory managers is to define when and how many of each item to replenish and how much to keep in a safety stock. The increasing number of items with different demand patterns and specific characteristics requires different policies to ensure a good performance in this area. Based on a case study of a chemical company, this paper proposes a method for classifying materials with different demand patterns into families, using specific replenishment and safety stock policies aimed at maintaining a balance of stocks and meeting the service levels required for production.

  15. Comparative genotoxic effects of the cooked-food-related mutagens Trp-P-2 and IQ in bacteria and cultured mammalian cells

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, L.H.; Carrano, A.V.; Salazar, E.; Felton, J.S.; Hatch, F.T.

    1983-01-01

    As part of a major study to evaluate the mutagenicity of chemicals produced during the cooking of foods, we examined the responses of bacteria and cultured Chinese hamster cells to the compounds Trp-P-2 (3-amino-1-methyl-5H-pyrido(4,3-b)indole) and IQ (2-amino-3-methylimidazo(4,5-f)quinoline), constituents identified in cooked beef and fish. In the Ames/Salmonella tester strain TA1538, both compounds were confirmed to be extremely potent mutagens that were active at levels below 1 ng/plate in the presence of hamster-liver S9 microsomal fraction. 50-fold higher doses of both compounds were required for mutagenicity in the uvr/sup +/ tester strain TA1978. Trp-P-2 also behaved as a strong mutagen in CHO cells using the standard exogenous activation with hamster-liver S9 fraction. At concentrations below 1 ..mu..g/ml it produced dose-dependent increases in cell killing, mutations at the hprt and aprt loci, sister-chromatid exchanges, and chromosomal aberrations. An excision-repair-deficient strain was about 2-fold more sensitive than the normal CHO cells with respect to these genotoxic effects of Trp-P-2. IQ had unexpectedly weak activity for all genetic endpoints in the CHO cells, and it produced clear-cut responses only in the repair-deficient cells and only above a concentration of 10 ..mu..g/ml. The toxicity that was observed with IQ was not affected by the repair capacity of the cells and was not associated with chromosomal aberrations, indicating that damage to cellular structures other than nuclear DNA was likely the predominant pathway for cell killing. 38 references, 3 figures, 1 table.

  16. A study of possible mutagenicity of irradiated onion powder by salmonella/mammalian-microsome tests

    International Nuclear Information System (INIS)

    The Salmonella/mammalian-microsome mutagenicity test was applied to detect possible mutagenicity of onion powder irradiated with 5 and 10 kGy, resp., using 60Co gamma radiation source. Aqueous extracts and pepsin/pancreatin digests equivalent to 50 and 20 mg of onion powder per plate, resp., were tested with and without the rat liver microsomal fraction, with Salmonella typhimurium mutant strains TA 100, TA 1535, TA 98, TA 1537 and TA 1538. Within the limitations of the experimental conditions applied, no mutagenicity of irradiated onion powder was demonstrable. (author)

  17. Reproductive health control of the families with mutagenic exposures after Chernobyl disaster

    International Nuclear Information System (INIS)

    The aim of this work is to estimate the additional genetic risk (hazard) of mutagenic radiation for evacuated families in relatively ecologically favorable region. The reproductive characteristics, birth defects prevalence and antropometric data compare was held among newborns for 130 families before and after mutagenic exposure, when they were evacuated from radiation polluted region. The study results indicated no evidence of low-dose radiation exposure impact on realization of additional mutagenic burden in the liquidators and evacuees families through the extent of reproductive losses and CM

  18. Effect of smoking on the chromosome aberrations induced by environmental mutagens

    International Nuclear Information System (INIS)

    Chromosome aberration frequencies have increasingly been used for the study of various occupational and environmental exposures. Factors related to environmental in mutagens influence translocation yield. Smoking has impact on the translocation yield and is the most influential factor among the environmental mutagens to increase cancer incidence. In order to know how environmental mutagens affect the induction of translocations caused by smoking we analyzed translocations in the lymphocytes of smokers and nonsmokers in a large city, Beijing, a high natural background radiation area (HBRA) and in its control area (CA), remote villages in the south of China. The results of our analyses are reviewed in this presentation

  19. Manejo de plantas daninhas em maracujazeiro amarelo cultivado com adubação química e orgânica Weed management in yellow passion fruit cultivated with chemical and organic fertilization

    Directory of Open Access Journals (Sweden)

    J. Ogliari

    2007-12-01

    Full Text Available Objetivou-se, neste trabalho, avaliar diferentes formas de manejo de plantas daninhas na cultura do maracujazeiro, cultivado com adubação química e orgânica. O delineamento experimental foi em blocos casualizados, com 15 tratamentos, arranjados em esquema de parcelas subdivididas, com quatro repetições e 10 plantas úteis por parcela. Os tratamentos foram constituídos por três tipos de adubações na parcela (orgânica, química e química + orgânica e cinco manejos de plantas daninhas na subparcela (com capina, sem capina, diuron (pré + glyphosate (pós, diuron (pré + MSMA (pós e diuron (pré + (diuron + paraquat (pós. O diuron foi aplicado aos cinco dias antes do plantio das mudas, em todos os tratamentos com herbicida, variando apenas os herbicidas em pós-emergência; para cada um dos herbicidas das misturas avaliadas, foram feitas três aplicações, aos 45, 96 e 159 dias. O diuron em pré-emergência provocou sintomas de clorose nas folhas entre 20 e 26 dias após o plantio (DAP, sendo mais evidente no maracujazeiro cultivado com adubação química. Os tratamentos com diuron (pré e glyphosate (pós apresentaram melhor controle das plantas daninhas. Os tratamentos com adubação química + orgânica associados aos manejos com capina, diuron (pré + glyphosate (pós e diuron (pré + (diuron + paraquat (pós foram os que proporcionaram maior produtividade de frutos. No cultivo com adubação orgânica, o tratamento capinado foi o que proporcionou maior produtividade. No cultivo com adubação química, a produtividade foi maior no tratamento com diuron (pré + glyphosate (pós.The objective of this work was to evaluate different types of weed management in passion fruit culture under chemical and organic fertilization. The experimental design was in randomized blocks with 15 treatments, arranged in a splip-splot design, with four repetitions containing 10 plants each. The treatments consisted of three types of fertilization

  20. Atributos químicos e estabilidade de agregados sob diferentes culturas de cobertura em Latossolo do cerrado Chemical properties and aggregate stability under different cover crops in cerrado Oxisol

    Directory of Open Access Journals (Sweden)

    Glenio G. Santos

    2012-11-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o efeito de diferentes culturas de cobertura sobre os atributos químicos e a estabilidade de agregados de um Latossolo do cerrado, sob plantio direto. O estudo foi conduzido em área experimental na Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO. As culturas de cobertura avaliadas foram: braquiária, milho em consórcio com braquiária (integração lavoura-pecuária, guandu anão, milheto, capim mombaça, sorgo granífero, estilosantes e crotalária. As amostras foram coletadas em abril de 2005 e 2006. O delineamento experimental foi o de blocos completos ao acaso com quatro repetições e os tratamentos arranjados em esquema fatorial 8 x 2, sendo oito culturas de cobertura e duas profundidades de amostragem do solo: 0-0,10 e 0,10-0,20 m. As culturas de cobertura influenciam, de forma diferenciada, os valores de pH e os teores de cálcio, magnésio, alumínio, fósforo, potássio, cobre, zinco e ferro do solo. O tratamento estilosantes tem maior poder em acidificar o solo. A agregação do solo varia com as culturas de cobertura e com a profundidade.The objective of this study was to evaluate the effect of different cover crops on chemical properties and aggregate stability in a cerrado Oxisol under no-tillage. The study was carried out in Embrapa Rice and Beans, in Santo Antônio de Goiás, GO, Brazil. The cover crops evaluated were: Urochloa brizantha, Urochloa brizantha and corn in association (crop-livestock integrated, Cajanus cajan, Pennisetum glaucum, Panicum maximum, Sorghum bicolor, Stylosanthes guianensis and Crotalaria juncea. The soil samples were collected in April and September 2005 and April 2006. The experimental design was in completely randomized blocks with four replications and treatments arranged in factorial scheme 8 x 2, eight cover crops and two soil sampling depths, 0-0.10 and 0.10-0.20 m. The different cover crops affect pH values and calcium, magnesium, aluminum

  1. Impact of environmental exposures on the mutagenicity/carcinogenicity of heterocyclic amines

    International Nuclear Information System (INIS)

    Carcinogenic heterocyclic amines are produced from overcooked foods and are highly mutagenic in most short-term test systems. One of the most abundant of these amines, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), induces breast, colon and prostate tumors in rats. Human dietary epidemiology studies suggest a strong correlation between either meat consumption or well-done muscle meat consumption and cancers of the colon, breast, stomach, lung and esophagus. For over 20 years our laboratory has helped define the human exposure to these dietary carcinogens. In this report we describe how various environmental exposures may modulate the risk from exposure to heterocyclic amines, especially PhIP. To assess the impact of foods on PhIP metabolism in humans, we developed an LC/MS/MS method to analyze the four major PhIP urinary metabolites following the consumption of a single portion of grilled chicken. Adding broccoli to the volunteers' diet altered the kinetics of PhIP metabolism. At the cellular level we have found that PhIP itself stimulates a significant estrogenic response in MCF-7 cells, but even more interestingly, co-incubation of the cells with herbal teas appear to enhance the response. Numerous environmental chemicals found in food or the atmosphere can impact the exposure, metabolism, and cell proliferation response of heterocyclic amines

  2. Impact of Environmental Exposures on the Mutagenicity/Carcinogenicity of Heterocyclic Amines

    Energy Technology Data Exchange (ETDEWEB)

    Felton, J S; Knize, M G; Bennett, L M; Malfatti, M A; Colvin, M E; Kulp, K S

    2003-12-19

    Carcinogenic heterocyclic amines are produced from overcooked foods and are highly mutagenic in most short-term test systems. One of the most abundant of these amines, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), induces breast, colon and prostate tumors in rats. Human dietary epidemiology studies suggest a strong correlation between either meat consumption or well-done muscle meat consumption and cancers of the colon, breast, stomach, lung and esophagus. For over 20 years our laboratory has helped define the human exposure to these dietary carcinogens. In this report we describe how various environmental exposures may modulate the risk from exposure to heterocyclic amines, especially PhIP. To assess the impact of foods on PhIP metabolism in humans, we developed an LC/MS/MS method to analyze the four major PhIP urinary metabolites following the consumption of a single portion of grilled chicken. Adding broccoli to the volunteers' diet altered the kinetics of PhIP metabolism. At the cellular level we have found that PhIP itself stimulates a significant estrogenic response in MCF-7 cells, but even more interestingly, co-incubation of the cells with herbal teas appear to enhance the response. Numerous environmental chemicals found in food or the atmosphere can impact the exposure, metabolism, and cell proliferation response of heterocyclic amines.

  3. Immunological detection and quantification of DNA components structurally modified by alkylating carcinogens, mutagens and chemotherapeutic agents

    International Nuclear Information System (INIS)

    The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 109 to > 1010 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis and malignant transformation of mammalian cells. These alkylation products include 06-N-butyldeoxyguanosine, and 04-ethyldeoxythymidine. When used in a radioimmunassay, an antibody specific for 06-ethyldeoxyguanosine, for example, will detect this product at an 06-ethyldeoxyguanosine/deoxyguanosine molar ratio of approx. 3 x 10-7 in a hydrolysate of 100 ug of DNA. The limit of detection can be lowered further if the respective alkyldeoxynucleosides are separated by HPLC from the DNA hydrolysate prior to the RIA. The anti-alkyldeoxynucleoside monoclonal antibodies can also be used to visualize, by immunostaining and fluorescence microscopy combined with electronic image intensification, specific alkylation products in the nuclear DNA of individual cells, and to localize structurally modified bases in double-stranded DNA molecules by transmission electron microscopy

  4. Generation of gamma irradiation and EMS-induced mutant lines of the H7996 tomato (Solanum lycopersicum L.)

    International Nuclear Information System (INIS)

    Tomato (L.) is one of the most important vegetable crops grown worldwide for the fresh vegetable market and food processing industry. With the completion of the genome-sequencing projects in various crops, the major challenge will be determine the gene function. One approach is to generate and to analyze mutant phenotypes. The paper reports the generation of gamma-irradiated and ethy methane sulfonate (EMS)-treated mutant populations, identification and phenotypic characterization of dominant and visible mutations in tomato mutant lines. Mutant populations of tomato H7996 were created using physical (cobalt 60 gamma ray) and chemical EMS mutagens. Generally, based on high-throughput phenotypic characterization, mutations were observed on the plant habit, size, morphology, leaf and flower color and morphology and fruit characteristics. Specifically, the most common dominant and visible mutations noted in the M1 generation were monopodial, compact, short internodes, multi-branch plant type, light yellow and ghost leaf coloration, tiny and long pedicel leaf morphology and small or short plant size. In the M2 generation, homogeneous and segregating M2 families were selected to constitute the core set of visible tomato mutants. Initial bacterial wilt resistance (BWR) gene knockouts were also identified. The mutant lines will be used as a rich source of genetic materials for breeding and functional genomics of tomato. (author)

  5. Controle químico da helmintosporiose (Bipolaris incurvata em coqueiro cultivar 'Anão-verde' em condições de campo Chemical control of Bipolaris incurvata in coconut cv. 'Anão-Verde' under field conditions

    Directory of Open Access Journals (Sweden)

    Junior Cesar Modesto

    2004-08-01

    Full Text Available Avaliou-se a eficácia de fungicidas para o controle da helmintosporiose (Bipolaris incurvata do coqueiro, em condições de campo. O experimento foi conduzido em Ocauçu - SP, em plantas com 8 meses de idade. Foram realizadas três pulverizações com intervalo quinzenal, utilizando-se de 600 litros de calda/ha, com os ingredientes ativos: prothioconazole (0,100 e 0,150 L/ha, tebuconazole (0,150 e 0,200 L/ha e propiconazole (0,125 e 0,250 L/ha. A primeira pulverização foi realizada em plantas com sintomas foliares da doença, de modo que todas as plantas avaliadas tinham as folhas velhas como fonte de inóculo. O controle proporcionado pelos ingredientes ativos, foi avaliado em folhas jovens que, sob condições climáticas favoráveis, foram suscetíveis ao ataque do patógeno. Constatou-se que todos os fungicidas e doses utilizados foram eficientes no controle da helmintosporiose (Bipolaris incurvata.Helmintosporiose fungicide control efficacy was evaluated in coconut plants, at field conditions. The experiment was conducted in Ocauçu-SP (Brasil, using 8-months old plants. Three sprayings were applied, within 15 days intervals, with 600 liters of spray volume/ha. The treatments were defined by three doses of the following fungicides: prothioconazole (0.100 and 0.150 L/ha, tebuconazole (0.150 and 0.200 L/ha and propiconazole (0.125 and 0.250 L/ha. The first spray was in symptomatic plants, therefore every evaluated plant had its old leaves as the inoculum source. The disease index evaluation was done in young leaves, which are susceptible to this pathogen under favorable environment conditions. All the fungicides and their doses used were efficient in controlling helmintosporiose leaf spot, except for their respective check-treatments (control treatments.

  6. Evaluation of the genotoxic and mutagenic potentials of homeopathic Candida albicans solutions

    Directory of Open Access Journals (Sweden)

    André Luis Santos

    2011-09-01

    Full Text Available Background: Candida spp is naturally found in humans’ flora of skin, gastrointestinal and genitourinary tracts and, in general, up to 75% of the population does not have any symptom [1]. However, oral candidiasis is very common among HIV patients and patients undergoing chemotherapy. The treatment of oral candidiasis is necessary once the disease causes discomfort and dysphagia, resulting in poor nutrition, slow recovery, and prolonged hospital stay [2,3]. Preliminary results obtained by our group with a new biotherapic prepared from Candida albicans (Candida 30x showed a great potential to reduce the candida yeast adhesion rate when the epithelial cells were pre-treated. This study is currently being developed with the evaluation of mutagenic and genotoxic potentials of several homeopathic solutions. Aims: The goal of this study was to assess the genotoxic and mutagenic potentials of different homeopathic potencies of C. albicans. Methodology: One part of C. albicans yeast obtained from Brazilian patient’s blood [4] was diluted in 9 parts of sterile water. This sample was submitted to 100 mechanical succussions (approximately 3 Hz, using Autic® Brazilian machine, originating the first dilution (1x. Then, 1 ml of this solution was diluted in 9 ml of solvent, submitted to 100 succussions, obtaining 2x potency. This procedure was successively repeated to obtain 30x potency, according to Brazilian Homeopathic Pharmacopoeia [5]. By the same technique, water vehicle was prepared until 30x to be used as control. All samples were prepared in sterile and aseptic conditions, using laminar flow cabinet, class II and were stored in the refrigerator (8ºC. The samples 1x, 6x, 12x, 18x, 24x and 30x of C. albicans and water 30x (vehicle control were analysed by: the Inductest, which assesses the ability of physical or chemical agents to promote lysogenic induction as a reflection of damage in DNA

  7. Cytotoxicity and mutagenicity of opthalmic solution preservatives and UVA radiation in L5178Y cells

    International Nuclear Information System (INIS)

    Four preservatives used in ophthalmic solutions were tested for toxic and mutagenic potential in mouse lymphoma cells with and without exposure of cells to ultraviolet A (UVA) radiation. The preservatives tested were benzalkonium chloride (BAK), chlorhexidine, thimerosal and ethylenediaminetetraacetic acid (EDTA). Cell survival and mutagenesis were measured using the L5178Y mouse lymphoma (TK+/-) system. Cells were exposed to varying amounts of preservatives for 1 h at 370C, and aliquots irradiated with UVA radiation (during exposure to preservative). Cells were then assayed for survival, and mutagenesis at the thymidine kinase (TK) locus. In concentrations commonly found in ophthalmic solutions, BAK, chlorhexidine, and thimerosal were toxic to cells, and thimerosal was slightly mutagenic. When cells were exposed to preservative and UVA radiation, chlorhexidine was mutagenic and the mutagenic activity of thimerosal was enhanced. (author)

  8. Cytotoxicity and mutagenicity of opthalmic solution preservatives and UVA radiation in L5178Y cells

    Energy Technology Data Exchange (ETDEWEB)

    Withrow, T.J.; Brown, N.T.; Hitchins, V.M.; Strickland, A.G. (Food and Drug Administration, Rockville, MD (USA). Center for Devices and Radiological Health)

    1989-09-01

    Four preservatives used in ophthalmic solutions were tested for toxic and mutagenic potential in mouse lymphoma cells with and without exposure of cells to ultraviolet A (UVA) radiation. The preservatives tested were benzalkonium chloride (BAK), chlorhexidine, thimerosal and ethylenediaminetetraacetic acid (EDTA). Cell survival and mutagenesis were measured using the L5178Y mouse lymphoma (TK{sup +/-}) system. Cells were exposed to varying amounts of preservatives for 1 h at 37{sup 0}C, and aliquots irradiated with UVA radiation (during exposure to preservative). Cells were then assayed for survival, and mutagenesis at the thymidine kinase (TK) locus. In concentrations commonly found in ophthalmic solutions, BAK, chlorhexidine, and thimerosal were toxic to cells, and thimerosal was slightly mutagenic. When cells were exposed to preservative and UVA radiation, chlorhexidine was mutagenic and the mutagenic activity of thimerosal was enhanced. (author).

  9. MUTAGENICITY IN SALMONELLA OF SULFUR-CONTAINING POLYCYCLIC AROMATIC HETEROCYCLES AND THEIR DIHYDRODIOL DERIVATIVES

    Science.gov (United States)

    Polycyclic aromatic sulfur heterocycles (PASH) are common constituents of cigarette smoke, fossil fuel-derived materials, and their combustion byproducts. Many PASH are known mutagens and carcinogens. However, unlike their nonsulfur-containing counterparts, relatively little is k...

  10. Mutagenicity of diesel exhaust particle extracts: influence of driving cycle and environmental temperature.

    Science.gov (United States)

    Clark, C R; Dutcher, J S; Brooks, A L; McClellan, R O; Marshall, W F; Naman, T M

    1982-01-01

    General Motors and Volkswagen diesel passenger cars (1980 and 1981 model year) were operated on a climate controlled chassis dynomometer and the particulate portion of the exhaust was collected on high volume filters. Dichloromethane extracts of the exhaust particles (soot) collected while the cars were operated under simulated highway, urban and congested urban driving cycles were assayed for mutagenicity in Salmonella strains TA-98 and TA-100. Driving pattern did not significantly influence the mutagenic potency of the exhaust particle extracts or estimates of the amount of mutagenicity emitted from the exhaust despite large differences in particle emission rates and extractable fraction of the particles. Mutagenicity of extracts of exhaust particles collected while the vehicles were operated at test chamber temperatures of 25, 50, 75 and 100 degrees F were also very similar. The results suggest that driving pattern and environmental temperature do not significantly alter the emission of genotoxic combustion products from the exhaust. PMID:6193022

  11. Absence of mutagenic effect of Mikania glomerata hydroalcoholic extract on adult wistar rats in vivo

    Directory of Open Access Journals (Sweden)

    Rita de Cássia da Silveira e Sá

    2006-07-01

    Full Text Available This work makes an assessment of the dominant lethality of Mikania glomerata in male Wistar rats. Adult male received 1 mL of M. glomerata hydroalcoholic extract at a dose level of 3.3 g/kg body weight for 52 days and were mated with untreated females for seven weeks (group 1 or one week prior to the beginning of treatment and on the week following the end of treatment (group 2. The parameters analyzed were: number of implanted embryos, resorptions and corpora lutea; mating, gestation, preimplantation loss, implantation and resorption indexes (group 1; number of offspring and weaning animals (group 2. The administration of M. glomerata did not show any impairment of fertility and no significant difference in the parameters analyzed, suggesting an absence of mutagenic effect on Wistar rats.Mikania glomerata é uma planta utilizada na medicina popular, cujas folhas possuem flavonóides e cumarina. Essas substâncias, segundo a literatura, interferem na fertilidade de cães e ratas, respectivamente. O presente trabalho faz um estudo do teste do letal dominante com M. glomerata em ratos Wistar. Animals adultos foram tratados com 1 mL de extrato hidroalcoólico de M. glomerata na dose de 3.3 g/kg de peso corporal durante 52 dias. Os animais foram acasalados com fêmeas não tratadas por sete semanas (grupo 1 ou uma semana antes do início do tratamento e na semana seguinte ao término do mesmo (grupo 2. As variáveis analisadas foram: números de embriões implantados, reabsorções e corpos lúteos, índices de acasalamento, gestação, perda pré-implantação, implantação e reabsorção (grupo 1; número de filhotes nascidos e de animais desmamados (grupo 2. A administração de M. glomerata não interferiu com a fertilidade dos animais e não foram observadas alterações significativas das variáveis analisadas, o que sugere a ausência de efeito mutagênico em ratos Wistar por parte dessa planta.

  12. Strong mutagenic effects of diesel engine emissions using vegetable oil as fuel

    Energy Technology Data Exchange (ETDEWEB)

    Buenger, Juergen; Bruening, Thomas [Institute of the Ruhr University Bochum, Research Institute for Occupational Medicine of the Institutions for Statutory Accident Insurance and Prevention (BGFA), Bochum (Germany); Krahl, Juergen [University of Applied Sciences Coburg, Coburg (Germany); Munack, Axel; Ruschel, Yvonne; Schroeder, Olaf [Institute for Technology and Biosystems Engineering, Federal Agricultural Research Centre (FAL), Braunschweig (Germany); Emmert, Birgit; Westphal, Goetz; Mueller, Michael; Hallier, Ernst [University of Goettingen, Department of Occupational and Social Medicine, Goettingen (Germany)

    2007-08-15

    Diesel engine emissions (DEE) are classified as probably carcinogenic to humans. In recent years every effort was made to reduce DEE and their content of carcinogenic and mutagenic polycyclic aromatic compounds. Since 1995 we observed an appreciable reduction of mutagenicity of DEE driven by reformulated or newly designed fuels in several studies. Recently, the use of rapeseed oil as fuel for diesel engines is rapidly growing among German transportation businesses and agriculture due to economic reasons. We compared the mutagenic effects of DEE from two different batches of rapeseed oil (RSO) with rapeseed methyl ester (RME, biodiesel), natural gas derived synthetic fuel (gas-to-liquid, GTL), and a reference diesel fuel (DF). The test engine was a heavy-duty truck diesel running the European Stationary Cycle. Particulate matter from the exhaust was sampled onto PTFE-coated glass fibre filters and extracted with dichloromethane in a soxhlet apparatus. The gas phase constituents were sampled as condensates. The mutagenicity of the particle extracts and the condensates was tested using the Salmonella typhimurium/mammalian microsome assay with tester strains TA98 and TA100. Compared to DF the two RSO qualities significantly increased the mutagenic effects of the particle extracts by factors of 9.7 up to 59 in tester strain TA98 and of 5.4 up to 22.3 in tester strain TA100, respectively. The condensates of the RSO fuels caused an up to factor 13.5 stronger mutagenicity than the reference fuel. RME extracts had a moderate but significant higher mutagenic response in assays of TA98 with metabolic activation and TA100 without metabolic activation. GTL samples did not differ significantly from DF. In conclusion, the strong increase of mutagenicity using RSO as diesel fuel compared to the reference DF and other fuels causes deep concern on future usage of this biologic resource as a replacement of established diesel fuels. (orig.)

  13. Intakes of red meat, processed meat, and meat-mutagens increase lung cancer risk

    OpenAIRE

    Lam, Tram Kim; Cross, Amanda J; Consonni, Dario; Randi, Giorgia; Bagnardi, Vincenzo; Bertazzi, Pier Alberto; Caporaso, Neil E.; Sinha, Rashmi; Subar, Amy F.; Landi, Maria Teresa

    2009-01-01

    Red and processed meat intake may increase lung cancer risk. However, the epidemiologic evidence is inconsistent and few studies have evaluated the role of meat-mutagens formed during high cooking temperatures. We investigated the association of red meat, processed meat, and meat-mutagen intake with lung cancer risk in Environment And Genetics in Lung cancer Etiology (EAGLE), a population-based case-control study. Primary lung cancer cases (n=2101) were recruited from 13 hospitals within the ...

  14. Human urinary mutagenicity after wood smoke exposure during traditional temazcal use

    OpenAIRE

    Long, Alexandra S.; Lemieux, Christine L; Yousefi, Paul; Ruiz-Mercado, Ilse; Lam, Nicholas L.; Orellana, Carolina Romero; White, Paul A.; SMITH, KIRK R.; Holland, Nina

    2014-01-01

    In Central America, the traditional temazcales or wood-fired steam baths, commonly used by many Native American populations, are often heated by wood fires with little ventilation, and this use results in high wood smoke exposure. Urinary mutagenicity has been previously employed as a non-invasive biomarker of human exposure to combustion emissions. This study examined the urinary mutagenicity in 19 indigenous Mayan families from the highlands of Guatemala who regularly use temazcales (N = 32...

  15. Mutagenic specificity of solar UV light in nucleotide excision repair-deficient rodent cells.

    OpenAIRE

    Sage, E.; Lamolet, B; Brulay, E; Moustacchi, E; Chteauneuf, A; Drobetsky, E A

    1996-01-01

    To investigate the role of nucleotide excision repair (NER) in the cellular processing of carcinogenic DNA photoproducts induced by defined, environmentally relevant portions of the solar wavelength spectrum, we have determined the mutagenic specificity of simulated sunlight (310-1100 nm), UVA (350-400 nm), and UVB (290-320 nm), as well as of the "nonsolar" model mutagen 254-nm UVC, at the adenine phosphoribosyltransferase (aprt) locus in NER-deficient (ERCC1) Chinese hamster ovary (CHO) cell...

  16. Avaliação das propriedades físicas, químicas e sensorial de preferência de goiabas em calda industrializadas Physical, chemical and sensorial analyses of industrialized guava in syrup

    Directory of Open Access Journals (Sweden)

    A. C. K. Sato

    2004-12-01

    Full Text Available Quatro marcas de goiabas em calda foram submetidas a análises físico-químicas, de cor e textura instrumentais e teste sensorial de preferência, a fim de avaliar as diferenças entre os produtos comerciais. As mesmas análises foram efetuadas em uma goiaba in natura madura, a fim de avaliar os possíveis efeitos do processamento na qualidade do produto. De maneira geral, observou-se grandes diferenças entre os produtos das diferentes marcas, e muitas vezes entre os frutos de um mesmo fabricante. As análises químicas mostraram que o teor de açúcares da maior parte dos produtos está entre 22 e 30%, com exceção de uma amostra que mostrou um valor bem mais elevado (ao redor de 40%. A análise sensorial mostrou que esta amostra com maior teor de açúcares foi a mais preferida em relação à doçura. Na análise de cor foram observados menores valores de a* em relação à fruta in natura, possivelmente devido à degradação dos carotenóides da goiaba. A luminosidade das goiabas em calda também é maior que da fruta in natura, devido à presença dos açúcares. Esta entrada de açúcares na fruta junto com o processamento térmico utilizado pode ter causado possível gelificação da pectina, aumentando a dureza, firmeza e elasticidade de algumas amostras processadas, quando comparadas à fruta fresca. No entanto, em alguns casos foi visível o efeito de processamentos mais drásticos, que levaram à perda da estrutura celular, observada pelos baixos valores das propriedades mecânicas. Em relação à textura e aparência, a amostra mais dura foi a preferida.Four brands of canned guava were submitted to physico-chemical, sensory (preference test and instrumental color and texture analyses, in order to evaluate the differences between commercial products. The same analyses were made on a fresh, mature guava, to evaluate possible effects of processing on the quality of the final product. There were considerable differences between the

  17. Composição química, avaliação físico-química e nutricional e efeito da expansão do milho e do farelo de soja para suínos em crescimento Chemical composition, physical-chemical and nutritional evaluation and effect of expanded corn and soybean meal on growing swine

    Directory of Open Access Journals (Sweden)

    J.A.F. Veloso

    2005-10-01

    Full Text Available Determinaram-se a composição química e as propriedades físico-químicas, a digestibilidade fecal dos nutrientes e os valores energéticos do milho, do milho expandido, do farelo de soja e do farelo de soja expandido, em suínos em crescimento. Foram feitas análises de rotina (análise proximal e de energia bruta e análises específicas, como gelatinização do amido, nitrogênio insolúvel em detergente ácido, atividade ureática, solubilidade protéica em KOH 0,2% e microscopia eletrônica de varredura. Na determinação da digestibilidade, usaram-se 20 leitões machos castrados, com peso inicial médio de 33,630±1,011kg, distribuídos em delineamento experimental inteiramente ao acaso, com cinco tratamentos e quatro repetições por tratamento. Uma dieta basal foi substituída por 40% dos alimentos energéticos ou por 20% dos protéicos. Nos alimentos expandidos, houve modificações no perfil químico e nas propriedades físico-químicas que influenciaram a digestibilidade dos nutrientes, em relação aos alimentos in natura. Ocorreu gelatinização do amido, incrementando (P0,05. O processo da expansão atuou diferentemente nas avaliações de laboratório e de digestibilidade dos nutrientes do milho e do farelo de soja expandidos.Growing pigs were used in a trial to evaluate chemical composition, physical-chemical properties, fecal digestibility of nutrients and energetic values for corn, expanded corn, soybean meal and expanded soybean meal. Proximate analyses, crude energy and specific analyses, as starch gelatinization, acid detergent insoluble nitrogen, urea activity, 0.2% KOH protein solubility and scanning electronic microscopy were performed. Twenty growing castrated pigs averaging 33.630±1.011kg of live weight were used in a digestibility assay. The pigs were allotted to different metabolic cages, in a completely randomized design with five treatments and four replicates. The energetic feed substituted 40% and the proteic

  18. Mutagenic, antimutagenic, cytotoxic, and apoptotic activities of extracts from Pituranthos tortuosus.

    Science.gov (United States)

    Abdelwahed, Afef; Skandrani, Ines; Kilani, Soumaya; Neffati, A; Sghaier, Mohamed Ben; Bouhlel, Ines; Boubaker, Jihed; Ammar, Rebaï Ben; Mahmoud, Amor; Ghedira, Kamel; Chekir-Ghedira, Leila

    2008-01-01

    Mutagenic and antimutagenic activities against direct acting mutagens, nifuroxazide (NF) and sodium azide (SA), and indirect acting mutagen aflatoxin B1 (AFB1) of extracts prepared from aerial parts of Pituranthos tortuosus were investigated in bacterial assay systems (i.e., the Ames test with Salmonella typhimurium TA100, TA98, TA1538, TA1535, and the SOS chromotest with Escherichia coli PQ 37). It was found that all extracts obtained from P. tortuosus decreased the mutagenicity induced by AFB1 (10 microg/assay), SA (1.5 microg/assay), and NF (20 microg/assay). Ethyl acetate, acetone, methanol, and total oligomer flavenoid extracts exhibited the highest inhibition level of mutagenicity induced by the indirect mutagen AFB1. In addition, antiproliferative and apoptotic properties of these extracts have also been reported using two leukemia cell lines, L1210 and K562. The results revealed that all extracts showed a significant cytotoxic effect on these cell lines, and the effect was greater in the presence of human K562 chronic myelogenous leukemia cells, whereas they do not induce apoptosis. PMID:18161507

  19. 32P-postlabelling analysis of DNA adducted with urinary mutagens from smokers of black tobacco.

    Science.gov (United States)

    Peluso, M; Castegnaro, M; Malaveille, C; Talaska, G; Vineis, P; Kadlubar, F; Bartsch, H

    1990-08-01

    In order to characterize the tobacco-derived mutagens excreted in the urine of tobacco smokers, 32P-postlabelling techniques were used to examine DNA adducts formed from these mutagens with calf thymus DNA in the presence of a metabolic activation system (rat liver S9, Aroclor 1254-induced, with or without acetyl coenzyme A). Using either nuclease P1 or butanol extraction procedures, four-six and three spots, respectively, were reproducibly found on the autoradiograms in the case of the urine extract from two smokers of black tobacco. Using the urinary extract from a non-smoker, only three faint spots were detected after nuclease P1 enrichment. DNA adducts produced in smokers' urine were then compared with those formed by four N-hydroxyarylamines, N-hydroxy-2-amino-3,8-dimethyl-3H-imidazo[4,5-f]quinoxaline, N-hydroxy-2-amino-3-methyl-imidazo[4,5-f]quinoxaline, N-hydroxy-2-naphthylamine and N-hydroxy-4-aminobiphenyl. Visual inspection revealed that none of the reference aromatic amines contributed to the adduct pattern produced by the urinary mutagen(s). However, primary aromatic amines are mainly implicated as urinary mutagens because: (i) they produce frameshift mutations in Salmonella typhimurium strains, (ii) they are easily extractable with blue cotton and (iii) their mutagenicity is abolished by a nitrite treatment procedure for deamination. PMID:2387016

  20. The metabolic activation of 2-naphthylamine to mutagens in the Ames test.

    Science.gov (United States)

    Tong, S; Smith, J; Manson, D; Gorrod, J W; Ioannides, C

    1986-01-01

    The mutagenicity of 2-naphthylamine and its major metabolites in the Ames test was determined in the absence and presence of activation systems derived from rats and hamsters. In the absence of an activation system only N-hydroxy-2-naphthylamine and 2-nitrosonaphthalene were mutagenic. In the presence of activation systems derived from control and 3-methylcholanthrene-treated hamsters and Arochlor 1254-treated rats only 2-naphthylamine exhibited a mutagenic response while its hydroxy-derivatives and 2-acetamidonaphthalene were devoid of mutagenicity. Pretreatment of rats with 3-methylcholanthrene or Arochlor 1254 enhanced the mutagenicity of 2-naphthylamine while treatments with phenobarbitone, safrole and clofibrate had no significant effect. In the hamster treatment with only 3-methylcholanthrene increased the mutagenicity of 2-naphthylamine. It is concluded that the metabolic activation of 2-naphthylamine proceeds via N-hydroxylation which is preferentially catalysed by the 3-methylcholanthrene inducible forms of cytochrome P-450, whereas ring-hydroxylation appears to be a deactivation pathway. PMID:3800318

  1. In vivo cytogenetic effects of the cooked-food-related mutagens Trp-P-2 and IQ in mouse bone marrow

    Energy Technology Data Exchange (ETDEWEB)

    Minkler, J.L.; Carrano, A.V.

    1984-01-01

    Sister-chromatid exchange and chromosomal aberrations were measured in vivo in mouse bone marrow following intraperitoneal injection of the cooked food mutagens, 3-amino-1-methyl-5H-pyrido(4,3-b)indole (Trp-P-2), and 2-amino-3-methylimidazo(4,5-f)quinoline (IQ). Trp-P-2 produced a significant positive dose response for both endpoints while IQ produced only a weak but significant sister-chromatid exchange response. The relative potency of these two chemicals is similar to that seen in mammalian cells in vitro but opposite to their potency in Salmonella.

  2. Phytochemical and Biological Studies of <em>Agave em>attenuata>

    OpenAIRE

    Vincenzo De Feo; Muhammad Riaz; Nasir Rasool; Muhammad Zubair; Komal Rizwan; Muhammad Zia-Ul-Haq

    2012-01-01

    The present study was conducted to examine various biological activities of a methanol extract of <em>Agave attenuataem> leaves. GC-MS analysis of the <em>n>-hexane fraction from the extract revealed the presence of 31 compounds, with mono-2-ethylhexyl phthalate (11.37%), 1,2-benzenedicarboxylic acid (6.33%), <em>n>-docosane (6.30%) and eicosane (6.02%) as the major components. The leaves contained appreciable levels of total phenolic contents (10...

  3. Eficiência e custo do controle químico da mancha de alternaria em tangor murcote Efficiency and cost of chemical control of alternaria brown spot

    OpenAIRE

    Adimara Bentivoglio Colturato; Tatiana Paulossi; Wilson Story Venâncio; Edson Luiz Furtado

    2009-01-01

    A mancha de alternaria, causada por Alternaria alternata f. sp. citri, afeta tangelos Minneola, tangerinas Dancy, tangores Murcote e, menos freqüentemente, tangelos Orlando, tangerinas Novas, Lees e Sunburst. Esta doença causa desfolha grave, queda de frutos e manchas nas frutas. O objetivo deste trabalho foi estabelecer o melhor fungicida e a melhor dose para o controle da mancha marrom de alternaria. O delineamento experimental foi de parcelas subdivididas em blocos, com 10 tratamentos prin...

  4. A descriptive mutagenicity assessment of tretinoin in Allium sativum

    International Nuclear Information System (INIS)

    This paper is primarily designed to assess the mutagenicity of tretinoin by applying the Allium test. Furthermore, it has the following objectives: to evaluate the macroscopic abnormalities caused by tretinoin based on root length and root form parameters; to investigate whether tretinoin can induce aberrances in cell division such as the formation of micronucleus, anaphase bridges, early anaphase, C-metaphase, sticky chromosome, stretched chromosome, vagrant chromosome and precocious chromosome; to determine the variation in the aberrations in the different concentration of tretinoin. Procedure: eight hundred equal-sized garlic bulbs were immersed in various concentrations of tretinoin and in tap water as control. These were divided into two groups. Six hundred bulbs were evaluated for macroscopic parameters while the remaining two hundred bulbs were fixed for microscopic observations. The Allium test set-ups were placed in the plant laboratory of UP-Manila. The were harvested on the third and on the fifth day. The fixed roots were examined in the Cytogenetics Laboratory of the Philippine Nuclear Research Institute. The data gathered for macroscopic parameter was statistically tested using Complete Randomized Design and the Tukey's Honestly Significant Difference. The microscopic abnormalities were determined descriptively for every concentration. Findings: analysis of macroscopic and microscopic parameters showed that: according to the analyses of variances, the number of roots, the root length and the number of root forms such as straight, bent, bulbous and tapered were not equal in all concentrations. However, the difference in the number of curled roots was not significant.; the root length distinctly showed the toxicity effect of tretinoin. The growth or the length of roots decreases as the tretinoin concentration increases; the mitotic abnormalities observed in the garlic cells include micronucleus, anaphase bridges, early anaphase. C-metaphase, sticky

  5. Mutagenic action of UV radiation on lambda prophage

    International Nuclear Information System (INIS)

    The lethal and mutagenic effects of UV radiation on a thermoinducible prophage lambda cI857 were studied in the wild-type cells of Escherichia coli K12 and in 9 repair-deficient mutants: uvrA6, uvrD3, uvrE502, polA1, recA13, lexA102, recB21recC22sbcB15, recB21recC22sbcB15recF143 and recB21recC22sbcB15recL152. After UV irradiation, lysogenic cells were submitted to thermal induction either immediately or after 90 min incubation in broth at 320. We scored for temperature-independent c-mutants of lambda phage that formed clear plaques at 320. After immediate thermoinduction (ITI) of prophage the phenomena were similar to W-reactivation (WR) and W-mutagenesis (WM) of UV-irradiated extracellular lambda phage infecting UV-irradiated host cells. In the wild-type host a shoulder was manifested on survival curves, and the frequency of c mutations significantly increased, attained a sharp maximum at 120 J/m2 and subsequently decreased. The mutagenic action on prophage remained normal in uvrA, uvrD, polA and recBCsbcB mutants, but was strongly reduced in uvrE-, recBC-sbcB-recF- and recBC-sbcB-recL- lysogens. After delayed thermoinduction (DTI) of prophage in the wild-type host, survival increased, but mutation frequency declined (in comparison with ITI). DTI had the same effects in repair-deficient mutants recBCsbcB, uvrD and polA. The delay in thermoinduction of prophage had no effect on the uvrA- lysogen but a slight effect in uvrE- and recBC-sbcB- recF- hosts. In the recBC-sbcB-recL- lysogen the delay in prophage induction had an opposite, i.e. stimulating, effect on UV mutagenesis. In recA- or lexA- hosts the prophage yielded no c mutants after either ITI or DTI. (orig.)

  6. Características químicas do solo sob algodoeiro em área que recebeu água residuária da suinocultura Chemical soil properties under cotton using swine wastewater

    Directory of Open Access Journals (Sweden)

    Salomão de Sousa Medeiros

    2011-06-01

    Full Text Available A crescente geração de efluentes líquidos e o seu lançamento no meio ambiente tem-se constituído numa preocupação mundial devido aos impactos negativos gerados. O objetivo deste trabalho foi avaliar os possíveis impactos da aplicação do efluente proveniente da suinocultura, após seu tratamento, nos atributos químicos do solo, em área cultivada com algodão. O experimento foi realizado no Perímetro Irrigado Formoso, no município de Bom Jesus da Lapa, BA, em área experimental da Companhia de Desenvolvimento dos Vales do São Francisco e do Parnaíba - CODEVASF. Foram testados cinco tratamentos: MC - manejo convencional = água de "boa qualidade" + adubação química; ET100 = 100 % de efluente tratado; ET75:25 = 75 % de efluente tratado + 25 % de água boa; ET50:50 = 50 % de efluente tratado + 50 % de água boa; ET25:75 = 25 % de efluente tratado + 75 % de água boa. O delineamento experimental foi em blocos ao acaso com quatro repetições. Os atributos químicos do solo avaliados foram: pH, condutividade elétrica, teores de N, P, K, Ca, Mg, Na, Zn, Fe, Mn e Cu. Em geral, quanto aos atributos químicos do solo, a adoção do manejo com efluente tratado apresentou resultados semelhantes aos obtidos com o MC, favorecendo a melhoria da fertilidade do solo e constituindo-se em uma fonte alternativa de fertilização de baixo custo. O efluente tratado, independentemente do fator de diluição, também demonstrou ser uma fonte alternativa de água.The negative impacts of the increasing volume of wastewater and of its discharge into the environment have become a worldwide concern. This study assessed the potential impacts of using treated effluent from pig raising for cotton irrigation, to evaluate their effects on soil chemical properties, and compare the results with those under conventional management. The experiment was conducted in an experimental area of the Company for the Development of the San Francisco and Parnaíba valleys

  7. Perfil físico-químico de aguardente durante envelhecimento em tonéis de carvalho Chemical profile of aguardente - Brazilian sugar cane alcoholic drink - aged in oak casks

    Directory of Open Access Journals (Sweden)

    Mariana Branco de Miranda

    2008-12-01

    Full Text Available Avaliou-se por um período de 390 dias o perfil da composição química da aguardente sob envelhecimento em tonéis de carvalho de 20 L. O envelhecimento da aguardente em tonéis de madeira melhora a qualidade sensorial do destilado. As aguardentes envelhecidas foram analisadas aos 0, 76, 147, 228, 314 e 390 dias de armazenamento quanto às concentrações de etanol, acidez volátil, ésteres, aldeídos, furfural, álcoois superiores (n-propílico, isobutílico e isoamílicos, metanol, cobre, extrato seco, taninos e cor. Após os 390 dias de armazenamento, a aguardente apresentou maiores concentrações de acidez volátil, ésteres, aldeídos, furfural, álcoois superiores, congêneres, extrato seco e tanino. Sua coloração tornou-se amarelada. As concentrações de etanol e de metanol não se alteraram, e o teor de cobre apresentou ligeiro declínio. O envelhecimento da aguardente por 390 dias em tonéis de carvalho alterou a sua composição química, porém ela se manteve dentro de todos os padrões de qualidade estabelecidos pela legislação nacional em vigor.The chemical composition of aguardente - Brazilian sugar cane alcoholic drink - under aging during in 20 L oak casks was evaluated for 390 days. Aging sugar cane aguardente in wood casks improves the sensorial quality of the distillate. The concentrations of ethanol, volatile acidity, esters, aldehydes, furfural, higher alcohols (n-propylic, isobutylic and isoamylics, methanol, copper, dry extract, tannins, and color of the aged sugar cane aguardente were analysed at 0, 76, 147, 228, 314, and 390 days of storage. After 390 days of aging the sugar cane aguardente presented higher concentrations of volatile acidity, esters, aldehydes, furfural, higher alcohols, congeners, dry extract, and tannin. Its color became golden. The concentrations of ethanol and methanol did not change and the copper content decreased slightly. The aging of the sugar cane aguardente in oak casks for 390 days

  8. Efeito de tratamentos químicos alternativos no controle de Diaphania spp. (Lepidoptera: Crambidae em pepino = Effect of alternative chemical treatments on the Diaphania spp. (Lepidoptera: Crambidae control in cucumber

    Directory of Open Access Journals (Sweden)

    Alvimar Bavaresco

    2007-07-01

    Full Text Available As brocas-das-cucurbitáceas Diaphania nitidalis e D. hyalinata são pragas importantes na cultura do pepino, danificando a parte aérea das plantas. Para seu controle, normalmente são empregados inseticidas de alta toxicidade e baixa seletividade aos inimigosnaturais. O objetivo deste trabalho foi avaliar o efeito de tratamentos químicos alternativos utilizados na produção orgânica no controle das brocas. Os tratamentos avaliados foram: 1. Bacillus thuringiensis var. kurstaki (Dipel PM®; 2. Óleo de nim (Dalneem®, 3. Extrato de sementes de cinamomo (Melia azedarach; 4. Extrato de folhas de cinamomo; 5. Extrato de folhas de pessegueiro bravo e pimenteira (Prunus sellowii e Capsicodendron dinisii; 6. Testemunha (sem aplicação, realizando-se quatro aplicações em intervalos de sete dias. A avaliação dos danos foi realizada sete dias após a 4ª aplicação. O inseticida biológico Dipel PM® (Bacillus thuringiesis var. kurstaki proporcionou maior eficiência de controle, reduzindoem 33,3% os danos em ponteiros e 25,9% em frutos de pepino. O óleo de nim (Dalneem® e os extratos aquosos de sementes de cinamomo, de folhas de cinamomo e de folhas de pessegueiro bravo + pimenteira não diferiram significativamente da testemunha, apresentando eficiência de controle de 0 a 22,2% em ponteiros e de 0,1 a 13,7% em frutos.The pickleworms Diaphania nitidalis and D. hyalinata are important pests of cucumber, damaging the canopy of the plants. In general, insecticides with high toxicity and low selectivity to natural enemies are used to control this pest. The objective of this work was to evaluate the effect of alternative chemical treatments on the control of pickleworms in cucumber. The evaluated treatments were: 1 – Bacillus thuringiensis var. kurstaki (Dipel PM®; 2 –Neem oil (Dalneem®, 3 – Chinaberry seeds extract (Melia azedarach; 4 – Chinaberry leaves extract; 5 – Prunus sellowii and Capsicodendron dinisii leaves extract; 6

  9. Mutagenic effects on indica rice carried by satellite

    International Nuclear Information System (INIS)

    Dried seeds of four indica rice varieties were carried into space by satellite Shijia No.8, the mutagenic effects of space condition on the seeds vigor and agronomic traits in the SP1 generation, and on the agronomic traits, amylose conent and bacterial resistance in the SP2 generation were studied. The results showed that the space condition slightly damaged rice seeds, with the physiological damage rate of germination rate, bud length, plant height and seed-setting rate in the SP1 ranged from 0 to 26.9%. Different varieties responded differently to the space conditions, and the order from strong to weak was Gui 99, Hanghui 7, R998, Jinhang 138. Compared with the control, no trait showed segregation in the SP1 generation. Some traits appeared larger segregation in the SP2 generation, and the mutants of plant height, number of tillers, weight of grain, amylose content and bacterial blight resistance were isolated in the SP2 generation, and these mutation traits could be inherited the SP3 generation. Space conditions not only produced mutants of rice agronomic traits, but also produced mutants of rice quality and disease resistance. (authors)

  10. ININ support to plant breeding using physical mutagens

    International Nuclear Information System (INIS)

    During 1974-1986 it was offered 171 irradiation services studies, multiplication and essays of yields in 32 different cultivations. The specialized personnel of the ININ has provided 31 advices about planning, experimental design and selection methods for such activities also it has realized studies over potato, sorghum, wheaten and broad beam from 1974. With the purpose for knowing the behavior of mutants, and segregative populations in several ecological niches, the ININ has ceded biological material to 10 institutions in some regions of the country. With the objective to achieve that the institutions which realize genetic improvement in Mexico use collaterally the ionizing radiations as mutagenic agent, the ININ with other participating institutions, has started an extension task throughout of country by means of activities such as: organization for annual meeting for knowing the advances of works of institutions that are using the irradiation for provoking mutagenesis. Conferences for investigators and professors to spread the own methodology. Imparting actualization courses for students of plant breeding. Finally the realization of courses for specialists with the backing of FAO/OIEA. (Author). 6 refs, 8 figs

  11. Mutagenic DNA repair in Escherichia coli: Pt. 18

    International Nuclear Information System (INIS)

    UV light was unable to induce rifampicin-resistant mutations at 430C in Escherichia coli ER11 and dnaE486. Although DnaE486 gene product is inactive at 430C, these bacteria contain the pcbA1 mutation which allows DNA replication provided DNA polymerase I is functional. The experiments were carried out under conditions where full expression of rifampicin-resistant mutations could occur so that the lack of induced mutations cannot be ascribed to an effect of incubation at 430C on mutation expression. UV-mutability at 43(0)C was restored by the presence of the dnaE+ allele on a plasmid. It is concluded that functional DnaE protein is essential for UV mutagenesis. The dnaE486 mutation also blocked the induction at 430C of mutations induced by UV plus delayed photoreversal, a procedure that has been postulated to reflect an early misincorporation step in the UV mutagenic process. (Author)

  12. Removal effect on Mesocyclops leukarti and mutagenicity with chlorine dioxide

    Institute of Scientific and Technical Information of China (English)

    ZUO Jin-long; CUI Fu-yi; QU Bo; ZHU Gui-bing

    2006-01-01

    Mesocyclops leukarti of zooplankton propagates excessively in eutrophic water body and it cannot be effectively inactivated by the conventional drinking water treatment process. In order to tackle this problem, a study of removal effect on Mesocyclops leukarti with chlorine dioxide in a waterworks was performed. The results showed that Mesocyclops leukarti could be effectively removed from water by 1.0 mg/L chlorine dioxide preoxidation combined with the conventional drinking water treatment process.Higher oxidizability and molecular state of chlorine dioxide in water is the key to the inactivation of Mesocyclops leukarti. The chlorite, disinfection by-products (DBPs) of chlorine dioxide, was stable at 0.45 mg/L, which is lower than that critical value of the USEPA. GC-MS examination showed that the quantity of organic substance in the water treated by chlorine dioxide obviously decreased. Ames test further revealed that the mutagenicity was reduced by chlorine dioxide with respect to prechlorine. The propagation ofMesocyclops leukarti can be inactivated effectively and safely by chlorine dioxide pre-oxidation.

  13. Studies on the possible mutagenicity of irradiated diets in rodents

    International Nuclear Information System (INIS)

    Recent protocols for the safety evaluation of processed foods have recommended the inclusion of tests to assess genetic damage. A nutritionally adequate diet containing items of both plant and animal origin and which had been irradiated (0.20 and 2.5 Mrad) to enable long term storage, has been tested for mutagenicity in mice and rats using the dominant lethal test. In the case of rats, males from the third successive generation of rats maintained on the irradiated whole diet was repeatedly paired with untreated virgin females for a week at a time. With mice, the males were fed the irradiated whole diet for 8 weeks and then paired for four consecutive weeks with batches of untreated virgin females. In all these studies there was no evidence of any increase in pre- and/or post-implantation loss arising from the feeding of irradiated whole diet. The frequency of females with dead implants and the pregnancy rate also remained unaltered. As assessed by dominant lethal test, the comsumption of feed exposed to even sterilising doses of gamma radiation is not associated with any genetic hazard. (author)

  14. Mutagenic activity of heavy metals in soils of wayside slopes

    Science.gov (United States)

    Fedorova, A. I.; Kalaev, V. N.; Prosvirina, Yu. G.; Goryainova, S. A.

    2007-08-01

    The genotoxic properties of soils polluted with heavy metals were studied on two wayside slopes covered with trees in the city of Voronezh. The nucleolar test in cells of the apical meristem of Zebrina pendula Schnizl. roots was used. The genotoxic effect of the soils was revealed according to the increased number of 2-and 3-nucleolar cells (from 41 to 54% and from 19 to 23% in the upper part of the first and second slopes, respectively; in the control, their number was 18 and 7%). The mean number of nucleoli per cell increased from 1.7 to 1.95 in the experiment and 1.31 in the control. The increased vehicle emissions, especially when cars go up the slopes (mainly in the upper and middle parts), correlated with the elevated heavy metal (Pb, Cu, Cd, and Zn) contents in the soil. The mutagenic substances may be removed to the Voronezh Reservoir, where they may be accumulated in some living organisms.

  15. Mutagenic effects of nitrogen and carbon ions on stevia

    International Nuclear Information System (INIS)

    Dry seeds of stevia were implanted by 60∼100 keV nitrogen ion and 75 keV carbon ion with various doses. The biological effects in M1 and mutation in M2 were studied. The results showed that ion beam was able to induce variation on chromosome structure and inhibited mitosis action in root tip cells. The rate of cells with chromosome aberration was increased with the increase of ion beam energy and dose. Energy effects of mitosis were presented between 75 keV and 60, 100 keV. As compared with γ-rays, the effects of ion beam were lower on chromosomal aberration but were higher on frequency of the mutation. The rate of cell with chromosome aberration and M2 useful mutation induced by implantation of carbon ion was higher than those induced by implantation of nitrogen ion. Mutagenic effects of Feng1 x Ri Yuan and of Ri Yuan x Feng2 are higher than that of Ji Ning and Feng2

  16. Formaldehyde in dentistry: a review of mutagenic and carcinogenic potential

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, B.B.; Chestner, S.B.

    1981-09-01

    For many years there has been controversy over the value of antimicrobial drugs for intracanal dressings in endodontics. Formocresol, a formaldehyde compound, has evolved as the preferred drug for routine endodontic procedures, as well as pediatric endodontics. The increase in the use of formaldehyde has been complicated by the introduction of paraformaldehyde pastes for filling root canals. Neither of these formulas has ever been standardized. The doses are arbitrary, and the common dose of formocresol has been shown to be many times greater than the minimum dose needed for effect. The efficacy of paraformaldehyde pastes is questionable and remains clouded by inconclusive evidence, conflicting research, inadequate terminology, and a lack of convincing statistical evidence. The clinical use and delivery of formocresol and paraformaldehyde pastes remain arbitrary and unscientific. Formaldehyde has a known toxic mutagenic and carcinogenic potential. Many investigations have been conducted to measure the risk of exposure to formaldehyde; it is clear that formaldehyde poses a carcinogenic risk in humans. There is a need to reevaluate the rationale underlying the use of formaldehyde in dentistry particularly in light of its deleterious effects.

  17. Induction of novel genetic recombinants through chemical mutagenesis of microspores in Indian Mustard B. juncea

    International Nuclear Information System (INIS)

    The microspore embryogenesis and DH production protocol developed in our lab [In Vitro Cellular and Development Biology - Plant. 2005, 41: 266-273] was used for microspores isolation and their chemical mutagenesis to widen the genetic base of three widely cultivated B. juncea species, Pusa Bold, Varuna and Bio- 902. The regenerated three to four leaf growth stage plantlets were diploidized, hardened and transplanted to develop doubled haploid plants. The microspores of genotype BIO-902 treated with either ENU/EMS did not produce any embryos while the control produced 85.4 ± 10.9 embryos/ Petri dish. Treatment with 5.0 μM ENU/EMS resulted in maximum embryo induction from the other two genotypes, Pusa Bold and Varuna. Irrespective of the concentration used, EMS mutated microspores produced embryos with higher frequency (239) as compared to those treated with ENU (106). The control embryos exhibited 85 to 90% germination against the mutant microspore derived embryos (16.7 to 31.5%). Overall lower concentrations of EMS (1.0 to 2.5 μM) compared to that of ENU (2.5 to 5.0 μM) promoted higher frequency of positive mutants with promising yield potential. Both EMS and ENU generated considerable variability for agro morphological and biochemical traits; appressed pod phenotype, number of pods, leaf size, total glucosinolate content and FA profile. Desirable phenotypes with reduced glucosinolate ( 100 μM) per g oil free meal were recovered from 2.5 to 5.0 μM EMS mutagenesis. Mutants with 45% oleic acid (against 40-45% erucic and 15-20% oleic in controls) were obtained in mutagenized plants from EMS (2.5 μM) and ENU (5.0 μM ). Useful variability was identified in mutant plants for their disease response to the most devastating fungal diseases Albugo candida (DI 0.6-2.0) and Alternaria brassicae (DI 1.3- 2.6) under artificial inoculation. (author)

  18. Relação entre macrofauna edáfica e atributos químicos do solo em diferentes agroecossistemas Relationship between edaphic macrofauna and soil chemical attributes in different agroecosystems

    Directory of Open Access Journals (Sweden)

    Sandra Santana de Lima

    2010-03-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do uso do solo sobre a densidade e a diversidade da macrofauna invertebrada, bem como a relação dessa com atributos químicos do solo em diferentes agroecossistemas. Foram estudados cinco agroecossistemas: sistema ecológico com três anos de adoção (SE3, sistemas agroflorestais com seis (SAF6 e dez (SAF10 anos de adoção; agricultura de corte e queima (ACQ, e floresta nativa (FN. Em cada sistema, foram coletadas aleatoriamente cinco amostras solo, em forma de blocos (25x25 cm, na profundidade de 10 cm, nas épocas seca (outubro, 2006 e chuvosa (maio, 2007. A relação entre os atributos químicos e a macrofauna edáfica, nos diferentes sistemas de uso do solo, foi determinada por meio da análise da coinércia. Maior abundância da macrofauna foi observada na época chuvosa. Os sistemas SE3, SAF6 e SAF10 apresentaram maior riqueza de espécies e índices de Shannon e Pielou, independentemente da época de coleta. O manejo agroflorestal favoreceu a ocorrência de "engenheiros do ecossistema". Os sistemas agroflorestais propiciam melhores características químicas do solo e aumentos na abundância e riqueza da macrofauna invertebrada do solo.The objective of this study was to evaluate land use effects on the abundance and diversity of invertebrate macrofauna and its relationship with the soil chemical characteristics in different agroecosystems. Five systems were studied: ecological based system at three years of adoption (ES3, agroforestry systems at six (AFS6 and ten years of adoption (AFS10, slash and burn agriculture (SBA, and native forest (NF. In each system, five samples were collected in the form of soil monoliths (25x25 cm at 10 cm depth, during the dry (October 2006 and rainy seasons (May 2007. The relationship between edaphic macrofauna and chemical attributes in the different land use systems was determined by coinertia analysis. The highest abundance of macrofauna was observed in

  19. Composição química do capim-elefante cv. Roxo cortado em diferentes alturas Chemical composition of elephant grass var. Roxo cut at different heights

    Directory of Open Access Journals (Sweden)

    Estácio Alves dos Santos

    2001-02-01

    Full Text Available O objetivo deste trabalho foi verificar o efeito dos cortes efetuados a 0, 15, 30 e 45 cm sobre a composição química do capim-elefante (Pennisetum purpureum, Schum. cv. Roxo, em épocas seca e chuvosa. As amostras foram obtidas de uma área útil de 8,4 m² de cada parcela. Após o corte de uniformização, efetuaram-se dois cortes no período seco, em intervalos de 90 dias, e três no período chuvoso, em intervalos de 60 dias. De cada parcela foi tomada uma amostra de 3 a 5 perfilhos, desidratada em estufa e triturada para analises laboratoriais. Foram avaliados os percentuais de matéria seca (MS, proteína bruta (PB, fibra em detergente neutro (FDN, fibra em detergente ácido (FDA, celulose (CEL, hemicelulose (HCEL, lignina (LIG e cinzas. As alturas de corte não influenciaram a composição química da forrageira, nem houve interação com as épocas. Com exceção de hemicelulose e cinzas, os cortes na época seca mostraram resultados superiores à chuvosa. As médias nas duas épocas foram 19,70 e 17,44% para MS; 7,74 e 7,25% para PB; 76,41 e 71,13% para FDN; 42,75 e 41,02% para FDA; 31,44 e 30,43% para CEL; 30,66 e 30,28% para HCEL; 9,25 e 7,83% para LIG; e 1,97 e 3,38% para cinzas, nos períodos seco e chuvoso, respectivamente.This work was carried out to evaluate the cutting effect at 0, 15, 30 and 45 cm height, on the chemical composition of elephant grass (Pennisetum purpureum, Schum. cv. Roxo in dry and rainy seasons. The samples were obtained from an useful area of 8,4 m² from each parcel. After the uniformity cut two cuts in dry season with 90 days interval, and three cuts in rainy seasons with 60 days interval. A sample of 3 or 5 tillers was taken from each parcel and they were dehydrated in air forced stove and ground for laboratorial analysis. The percentages of dry matter (DM, crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF, cellulose (CEL, hemicellulose (HCEL, lignin (LIG, and ashes were

  20. Rapid changes in peat fly ash mutagenicity after release into the atmosphere: a controlled dilution bag study

    Energy Technology Data Exchange (ETDEWEB)

    Jantunen, M.J.; Liimatainen, A.; Ramdahl, T.; Itkonen, A.

    1986-07-01

    Effects of moisture condensation on fly ash mutagenicity, when the effluent of a peat-fired power plant is dispersed into cold and humid air, were investigated. A 140-m/sup 3/ flow- type dilution bag was used to dilute flue gas collected after the electrostatic precipitator with ambient air and to age this mixture for approximately 2 min. Fly ash was sampled by size fractionating aerosol samplers pre- and postdilution. Organics were extracted from the samples, fractionated by semipreparative high-performance liquid chromatography, and assayed for mutagenicity by the Ames method (TA98 and TA100 with and without S9). The smallest particles were the most mutagenic in both pre-and postdilution samples. Mutagenicity of the fly ash increased strongly in the dilution bag. This was most pronounced in the medium- and low-polarity organic fractions. In the predilution samples most of the mutagenicity was found in the most polar fraction. Postdilution of this fraction showed a modest increase for TA98 and TA98 + S9 but not for TA100 or TA100 + S9. Direct mutagenicity and mutagenicity with activation were comparable. In the postdilution samples most of the mutagenicity was found in the nonpolar and medium polarity fractions. The mutagenicity of the nonpolarity and medium polarity fractions increased strongly after aging 2 min in the mixing bag. In these fractions predilution mutagenicity with and without activation was comparable. Following dilution and aging the mutagenicity with activation increased to a much greater extent than the direct mutagenicity. 21 references.

  1. Atributos químicos e estoques de carbono em Latossolo sob plantio direto no cerrado do Piauí Chemical attributes and carbon stocks in an Oxisol under no-tillage in savannah of Piauí state

    Directory of Open Access Journals (Sweden)

    Luiz F. C. Leite

    2010-12-01

    Full Text Available O sistema de plantio direto é uma alternativa viável para o uso sustentável dos solos. O objetivo do presente trabalho foi avaliar a influência do sistema plantio direto com diferentes tempos de implantação e do plantio convencional sobre os atributos químicos e os estoques de C de um Latossolo Vermelho-Amarelo do cerrado piauiense. Amostras de solo foram coletadas nas camadas de 0-5, 5-10, 10-20 e 20-40 cm em sistema de plantio convencional (PC e plantio direto (PD com 2 (PD2, 4 (PD4 e 6 (PD6 anos de implantação, além de uma área sob floresta nativa de cerrado (FNC. Foram determinados o pH em água, P extraível (Pext, bases trocáveis, Al, acidez potencial (H+Al, carbono orgânico total (COT e carbono da biomassa microbiana (Cmic. A adoção do sistema plantio direto aumentou o pH, os teores de P e de bases trocáveis do solo, em comparação com o PC. Em relação ao PC, os estoques de COT e Cmic aumentaram 34 e 99% no PD2, 47 e 92% no PD4 e 61 e 108% no PD6, respectivamente, na camada superficial de 0-20 cm. A implantação do sistema plantio direto melhora os atributos químicos e aumenta os estoques de carbono orgânico total e microbiano do solo e seu uso contínuo contribui para a qualidade do solo em áreas do cerrado piauiense.No-tillage adoption has been considered a viable alternative for sustainable soil use. This work aimed to evaluate the influence of no-tillage system with different times of adoption and conventional tillage on chemical attributes and carbon stocks in an Oxisol of the savannah of Piauí in Northeastern Brazil. Soil samples in the 0-5, 5-10, 10-20 and 20-40 cm layers were collected in conventional tillage (CT no-tillage (NT with 2 (NT2, 4 (NT4 and 6 (NT6 years of adoption and native forest (NF. The following chemical properties were evaluated: pH, extractable P, exchangeable cations, Al, potential acidity (H+Al, total organic carbon (TOC and microbial biomass carbon (Cmic. NT adoption increased p

  2. Saponins with Neuroprotective Effects from the Roots of <em>Pulsatilla cernuaem>

    OpenAIRE

    Wen-Hao Fan; Zong-Gui Yu; Hui Zhang; Yong-Nan Xu; Li-Bo Zou; Yi-Xia Gong; Hui-Ming Hua; Jian-Yu Liu; Ying-Li Guan

    2012-01-01

    Four new oleanene-type triterpenoid saponins together with six known saponins were isolated from the roots of <em>P>ulsatilla cernuaem> and their structures were elucidated on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. Among these one of the aglycones (gypsogenin) is reported for the first time from this genus. Some of these compounds showed significant neuroprotective effects against the

  3. Organic mutagens and drinking water in the Netherlands : a study on mutagenicity of organic constituents in drinking water in The Netherlands and their possible carcinogenic effects

    NARCIS (Netherlands)

    Kool, H.J.

    1983-01-01

    Several mutagenic and carcinogenic organic compounds have been detected in Dutch surface waters and in drinking water prepared from these surface waters. Although the levels of these compounds in drinking- and surface water are relatively low, in general below μg per litre, it appeared that organic

  4. Identification of Volatile Components of Liverwort (<em>Porella cordaeanaem> Extracts Using GC/MS-SPME and Their Antimicrobial Activity

    Directory of Open Access Journals (Sweden)

    Maria Elisabetta Guerzoni

    2012-06-01

    Full Text Available Chemical constituents of liverwort (<em>Porella cordaeanaem> extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS. The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%, and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%, respectively. The dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10% and β-caryophyllene (10.72%, 8.29%, 7.79%, respectively. The antimicrobial activity of the extracts was evaluated against eleven food microorganisms using the microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC varied from 0.50 to 2.00 mg/mL for yeast strains (<em>Saccharomyces cerevisiae 635em>, <em>Zygosacharomyces bailii 45em>, <em>Aerobasidium pullulans L6Fem>, <em>Pichia membranaefaciens OC 71em>, <em>Pichia membranaefaciens OC 70em>, <em>Pichia anomalaem> <em>CBS 5759em>, <em>Pichia anomala DBVPG 3003em> and<em> em>>Yarrowia lipolytica RO13em>, and from 1.00 to 3.00 mg/mL for bacterial strains<em> em>(Salmonella> <em>enteritidis 155em>, <em>Escherichia coli 555em> and <em>Listeria monocytogenes 56Lyem>. Methanol extract showed better activity in comparison with ethanol and ethyl acetate extracts. High percentages of monoterpene and sesquiterpene hydrocarbons could be responsible for the better antimicrobial activity.

  5. Breeding of Hordeum Vulgare L. via chemical and physical Mutagenesis

    International Nuclear Information System (INIS)

    A field experiment was conducted under field conditions to compare for yield, yield components and some agronomic traits, of four selective mutants that have been obtained by using chemical mutagen (Sodium Azide) and physical mutagen ( Gamma ray, 20 KG) and their parent Arivate and Numar. The results show that the selective mutants have a high degree of genetic stability and exceeded their parents in some agronomic parameters. There fore, the two best selective mutants were accepted for registration and release under the name of Barrak and Amel as a new varieties by Nacional Committee for Registration and Release of Agricultural varieties

  6. Características físico-químicas e químicas e estudo preliminar de estabilidade de tinturas preparadas com espécies de arnica Lychnophora em comparação com Arnica montana Physico-chemical and chemical characteristics and stability study of tincture from the arnicas Lychnophora and Arnica montana

    Directory of Open Access Journals (Sweden)

    Renata L. Maciel

    2006-03-01

    Full Text Available Arnica é o nome originalmente atribuído à espécie Arnica montana L.(Asteraceae, planta de origem Européia e utilizada em várias partes do mundo. A medicina tradicional brasileira atribui o nome de arnica também a outras espécies da família Asteraceae, especialmente algumas do gênero Lychnophora. No presente estudo foram determinadas as características físico-químicas (organoléptica, teor alcoólico, densidade, pH, porcentagem de resíduo seco e químicas (CCD e CLAE de tinturas preparadas com as arnicas L. pinaster e L. rupestris, em comparação com a A. montana. As amostras foram então submetidas a estudo de estabilidade, a partir da sua permanência em estufa climatizada por seis meses e em prateleira por 10 meses. Os resultados demonstraram alguma semelhança entre as tinturas de espécies de Lychnophora e a A. montana, especialmente entre seus perfis em CCD. Todas as tinturas sofreram alterações após o período de permanência na estufa, sendo o mais significante a redução no valor dos teores de resíduos secos, indicando degradação das substâncias e perda por volatilização. O conjunto das análises permitiu distinguir as tinturas de cada uma das espécies e confirmou a necessidade de determinar prazos de validade para as mesmas, devido à sua a instabilidade ao longo do tempo.Arnica is the name attributed to Arnica montana L. (Asteraceae, an european species used in several parts of the world. The Brazilian traditional medicine also attributes the name of arnica to other species of the Asteraceae, especially some Lychnophora. In the present study physical-chemical characteristics (organoleptic, alcoholic graduation, density, pH, percentage of dry residue and chemical (TLC and HPLC profiles of tinctures prepared with L. rupestris, L. pinaster and A. montana has been determined. The samples had been then submitted the thermal stability testing. The results demonstrated some similarity between tinctures of the species

  7. Métodos mecânicos e químico no controle de ervas daninhas em cafezal Mechanical and chemical methods of weed control in coffe crop

    Directory of Open Access Journals (Sweden)

    Gastão Moraes da Silveira

    1985-01-01

    Full Text Available O controle de ervas daninhas em cafezal cultivado em latossolo roxo, por meio de métodos mecânicos (grade de discos, enxada rotativa, roçadeira e químico (herbicida, é comparado com a capina manual. Cada implemento foi sistematicamente usado durante o período das águas. Quanto à produção, após dez anos, não houve diferença estatística entre os tratamentos, a não ser no biênio 1976/77, quando a grade e a enxada rotativa tiveram melhor comportamento, não diferindo do herbicida. Observaram-se sinais de erosão superficial provocada por grade de discos, enxada rotativa e herbicida.Mechanical (disc harrow, rotary cultivator, off-set rotary shredder, chemical (herbicide and hand hoe weeding are compared in a coffee crop grown on a latosol. After ten years there was no significant yield difference among the treatments. There was however, visible signs of erosion due to the use of disc-harrow, rotary cultivator, and herbicide.

  8. Amostragem e variabilidade espacial de atributos químicos do solo em área de vegetação natural em regeneração Sampling and spatial variability of chemical attributes of a soil under regenerating natural vegetation

    Directory of Open Access Journals (Sweden)

    Julião Soares de Souza Lima

    2010-02-01

    Full Text Available Este trabalho teve como objetivos estimar o número de subamostras de solo, considerando-se métodos de estatística clássica e geoestatística, e determinar a variabilidade espacial dos atributos de fertilidade de um Argissolo Vermelho-Amarelo, textura argilosa, em uma área de vegetação natural em processo de regeneração, no Município de Alegre, ES. Amostras de solo foram coletadas na profundidade de 0-0,20 m, nos pontos de cruzamento de uma malha com intervalos regulares de 10 m, perfazendo um total de 64 pontos. Observouse baixo nível de fertilidade do solo. Considerando uma variação de 5% em torno da média no método da estatística clássica, necessita-se de maior número de amostras em relação à geoestatística. Todos os atributos químicos apresentaram dependência espacial de moderada a alta, com exceção da capacidade efetiva de troca catiônica (CTCe, que apresentou efeito pepita puro. O modelo de semivariograma que mais se ajustou aos dados foi o esférico. Os mapas de isolinhas permitiram visualizar a distribuição espacial diferenciada dos teores dos atributos químicos do solo.The objectives of this work was to estimate the number of soil subsamples considering the classical statistics and geostatistics and determine the spatial variability of soil fertility attributes of an Ultisol, with clay texture, in an area of regenerating natural vegetation in Alegre - ES. Soil samples were collected in a depth of 0.0-0.2 m, at the crossing points of a regular grid, comprising a total of 64 points located at 10 m-intervals. The area presented low fertility soil. Considering a variation of 5% around the mean in the classic statistics, it is necessary a larger number of samples in relation to geostatistics. All the chemical attributes showed moderate to high spatial dependence, except for the effective cation exchange capacity (CECe, which showed pure nugget effect. The spherical semivariogram model gave the best fit to the

  9. Chemical analysis and biological testing of materials from the EDS coal liquefaction process: a status report

    Energy Technology Data Exchange (ETDEWEB)

    Later, D.W.; Pelroy, R.A.; Wilson, B.W.

    1984-05-01

    Representative process materials were obtained from the EDS pilot plant for chemical and biological analyses. These materials were characterized for biological activity and chemical composition using a microbial mutagenicity assay and chromatographic and mass spectrometric analytical techniques. The two highest boiling distillation cuts, as well as process solvent (PS) obtained from the bottoms recycle mode operation, were tested for initiation of mouse skin tumorigenicity. All three materials were active; the crude 800/sup 0 +/F cut was substantially more potent than the crude bottoms recycle PS or 750 to 800/sup 0/F distillate cut. Results from chemical analyses showed the EDS materials, in general, to be more highly alkylated and have higher hydroaromatic content than analogous SRC II process materials (no in-line process hydrogenation) used for comparison. In the microbial mutagenicity assays the N-PAC fractions showed greater activity than did the aliphatic hydrocarbon, hydroxy-PAH, or PAH fractions, although mutagenicity was detected in certain PAH fractions by a modified version of the standard microbial mutagenicity assay. Mutagenic activities for the EDS materials were lower, overall, than those for the corresponding materials from the SRC II process. The EDS materials produced under different operational modes had distinguishable differences in both their chemical constituency and biological activity. The primary differences between the EDS materials studied here and their SRC II counterparts used for comparison are most likely attributable to the incorporation of catalytic hydrogenation in the EDS process. 27 references, 28 figures, 27 tables.

  10. Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

    Directory of Open Access Journals (Sweden)

    A Della Torre

    2011-01-01

    Full Text Available Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins. The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9 and without (-S9 metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9 and showed signs of mutagenic activity in TA97a and TA102 (both -S9 strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

  11. Mutagenic and genotoxic activity of particulate matter MP2,5, in Pamplona, North Santander, Colombia

    Directory of Open Access Journals (Sweden)

    Martínez Montañez, Mónica Liseth

    2012-10-01

    Full Text Available Objective: To study the mutagenic and genotoxic activities of particulate material (MP2,5 collected in Pamplona, Norte de Santander, Colombia.Materials and methods: MP2,5 was monitored by means of a Partisol 2025 sequential air sampler with Plus Palmflex quartz filters. The latter were subjected to two extraction procedures: Soxhlet extraction using dichloromethane-acetone; and ultrasonic extraction using dichloromethane, acetone and dichloromethane/ acetone mix. The mutagenic and genotoxic activities were determined for each extract.Results: This is the first study conducted in Colombia that reports the mutagenic and genotoxic activities associated with particulate matter (MP2,5 taken from vehicular emissions in Pamplona, Norte de Santander. The mutagenic assay determined by the Ames test using Salmonella typhimurium strains TA98 and TA100 showed a high direct mutagenic activity in the analyzed extracts. On the other hand, the genotoxic activity, determined by means of the comet assay, was high too.Conclusion: Particulate material (MP2,5 present in air samples in Pamplona (northeastern Colombia is a risk factor for the exposed population because it can directly induce mutations and also cause genotoxic damage.

  12. Antioxidant, mutagenic, and antimutagenic activities of Tragopogon longirostis var. longirostis, an edible wild plant in Turkey

    Directory of Open Access Journals (Sweden)

    Nurdan Sarac

    2015-01-01

    Full Text Available Objectives: The ethanolic extract of Tragopogon longirostis var. longirostis, a wild edible plant in Anatolia was isolated, and its antioxidant, mutagenic, and antimutagenic properties were investigated. Materials and Methods: The antioxidant activity (AA was determined by the inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH radical, total AA, and phenolic compounds. The mutagenic and antimutagenic activities were investigated by Ames Salmonella/microsome mutagenicity test. Results: The IC 50 value for DPPH radicals was 7.84 ± 0.603 mg/mL. The total AA increased with an increase in the concentration of the extracts (1, 5, 10, 20, and 30 mg/mL, containing linoleic acid emulsion. The total phenolic content was 284.71 ± 5.6 mg gallic acid equivalent/g extract. The results showed that the ethanolic extract can be considered safe, because it does not have any mutagenic effect at the tested concentrations. As a result, the ethanolic extract of the leaves exhibited antimutagenic effects at 2.5, 0.25, and 0.025 mg/plate concentrations. Conclusions: To our knowledge, this is the first study of the antioxidant, mutagenic, and antimutagenic activities of T. longirostis var. longirostis. These activities are an important topic in the food industry, as well as in the medical field.

  13. Cashew (Anacardium occidentale apple juice lowers mutagenicity of aflatoxin B1 in S. typhimurium TA102

    Directory of Open Access Journals (Sweden)

    Ana Amélia Melo Cavalcante

    2005-01-01

    Full Text Available Cashew (Anacardium occidentale is a medicinal plant native to Brazil and also yields a nutritious fruit juice. Its large pulpy pseudo-fruit, referred to as the cashew apple, contains high concentrations of vitamin C, carotenoids, phenolic compounds and minerals. Natural and processed cashew apple juice (CAJ/cajuina are amongst the most popular juices in Brazil, especially in the north-east. Both juices have antioxidant potential and suppress mutagenicity of hydrogen peroxide. In the present study we evaluated the inhibitory effects of CAJ/cajuina on Aflatoxin B1(AFB1-induced mutation, using the Salmonella/microsome assay with the experimental approaches of pre-, co- and post-treatments. Both CAJ/cajuina suppress AFB1-induced mutagenesis in strain TA102 when applied in co- and in post-treatment. Possible mechanisms for anti-mutagenicity in co-treatment are (a interaction with S9 enzymes, (b metabolization to non-mutagenic compounds of AFB1 or (c inactivation of S9 potential. Total suppression of AFB1 mutagenicity was observed in co-treatment with both CAJ and cajuina. Post-treatment anti-mutagenicity of both juices suggests a modulation of activity of error-prone DNA repair. CAJ/cajuina may be considered promising candidates for control of genotoxicity of AFB1 and may thus be considered as health foods with anti-carcinogenic potential. This promising characteristic warrants further evaluation with in vivo studies.

  14. Wholesomeness studies on gamma-irradiated smoked fish using short-term mutagenicity assays

    International Nuclear Information System (INIS)

    The effect of gamma irradiation on the mutagenicity potential of wood-smoked mackerel (Rastrelliger sp.) was investigated. Smoked fish were irradiated with dose of 2.0, 4.0, 6.0 and 8.0 KGy, and tested for mutagenic activity using the Salmonella plate incorporation assay, host-mediated assay, and micronucleus test. The DMSO extract of unirradiated smoked fish was found to be mutagenic, without metabolic activation in Salmonella strains TA 100 and TA 104, both sensitive to base-pair substitution mutations. Strains TA 98 and TA 97 which are sensitive to frameshift mutations showed no mutagenic activity towards the same DMSO extract. The observed response towards the Salmonella strains was not affected by irradiation in the range of radiation doses studied. The presence of protamutagens in the DMSO extract of unirradiated smoked fish was not detected using the host-mediated assay. In another in-vivo test however, the same DMSO extract induced the formation of micronuclei in the bonemarrow cells of mice. Gamma irradiation up to a dose of 8.0 KGy did not affect the observed mutagenicity of wood-smoked fish. (author)

  15. Assessment of diphenylcyclopropenone for photochemically induced mutagenicity in the Ames assay

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, M.G.; Connor, T.H.; Henkin, J.; Wilkin, J.K.; Matney, T.S.

    1987-10-01

    The photochemical conversion of diphenylcyclopropenone to diphenylacetylene has recently been reported. Diphenylcyclopropenone is used in the treatment of alopecia areata and is nonmutagenic in a limited Ames assay. We examined diphenylcyclopropenone and diphenylacetylene, as well as synthetic precursors of diphenylcyclopropenone--dibenzylketone and alpha,alpha'-dibromodibenzylketone--for mutagenicity against TA100, TA98, TA102, UTH8413, and UTH8414. All compounds were nonmutagenic except alpha,alpha'-dibromodibenzylketone, which was a potent mutagen in TA100 with and without S-9 activation. The effect of photochemical activation of diphenylcyclopropenone in the presence of bacteria demonstrated mutagenicity in UTH8413 (two times background) at 10 micrograms/plate with S-9 microsomal activation. 8-Methoxypsoralen produces a mutagenic response in TA102 at 0.1 microgram/plate with 60 seconds of exposure to 350 nm light. In vitro photochemically activated Ames assay with S-9 microsomal fraction may enhance the trapping of short-lived photochemically produced high-energy mutagenic intermediates. This technique offers exciting opportunities to trap high-energy intermediates that may play an important role in mutagenesis. This method can be applied to a variety of topically applied dermatologic agents, potentially subjected to photochemical changes in normal use.

  16. Detection, transmission, pathogenicity and chemical treatment of fungi in Ceiba speciosa seeds Detecção, transmissão, patogenicidade e controle químico de fungos em sementes de paineira (Ceiba speciosa

    Directory of Open Access Journals (Sweden)

    Marília Lazarotto

    2010-06-01

    Full Text Available The aim of this work was to evaluate fungus association, transmission and pathogenicity, besides chemical seed treatment in Ceiba speciosa seeds from different regions of southern Brazil. Seven seed samples were used to do the germination test, fungus detection by blotter test and potato-dextrose-agar (PDA, fungus transmission and pathogenicity tests; besides, chemical seed treatments were tested. Germination ranged from 0 to 59,5%. The following fungi were associated in the seeds: Fusarium sp., Alternaria sp., Colletotrichum sp., Curvularia sp. and Pestalotia sp.; in addition, Fusarium sp. was found in all the samples. Alternaria sp. and Fusarium sp. were transmitted by seeds. The isolates of Alternaria sp., Colletotrichum sp. and Fusarium sp., were pathogenic to seedlings and seeds. The seed treatment with methyl tiophanate and the combination captan + methyl tiophanate reduced Fusarium sp. incidence.Neste trabalho avaliou-se a incidência, transmissão, patogenicidade de fungos e a eficiência do tratamento químico no controle dos mesmos em sementes de paineira (Ceiba speciosa oriundas de diferentes locais do sul do Brasil. Para tanto, utilizaram-se sete amostras de sementes, com as quais realizaram-se testes de germinação, sanidade empregando-se o método do papel-filtro (PF e de plaqueamento em batata-dextrose-ágar (BDA, transmissão e patogenicidade dos fungos e tratamento químico das sementes. A germinação variou de 0 a 59,5%. Foram detectados os seguintes fungos associados às sementes: Fusarium sp., Alternaria sp.,Colletotrichum sp., Curvularia sp. e Pestalotia sp.; sendo que Fusarium sp. foi encontrado em todas as amostras. Ambos os métodos de detecção, PF e BDA, foram eficientes na detecção da maior parte dos fungos. Alternaria sp. e Fusarium sp. foram transmitidos via sementes. Os isolados de Alternaria sp., Colletotrichum sp. e Fusarium sp. foram patogênicos às plântulas e os tratamentos das sementes com tiofanato met

  17. Variabilidade espacial de atributos químicos do solo em áreas sob sistema plantio convencional Spatial variability of soil chemical attributes in areas managed under conventional tillage

    Directory of Open Access Journals (Sweden)

    Leonardo de Oliveira Machado

    2007-06-01

    Full Text Available A variabilidade espacial de atributos químicos de solo foi avaliada em uma lavoura comercial cultivada sob sistema plantio convencional, em Uberlândia (MG, no ano de 2004. O objetivo foi avaliar a distribuição e a dependência espacial dos atributos químicos do solo em uma lavoura sob sistema plantio convencional. A grade de amostragem foi estabelecida na Fazenda Santa Rosa, sendo o solo classificado como Latossolo Vermelho textura muito argilosa (680 g kg-1 de argila. Coletaram-se dados do solo, dispostos segundo uma malha de 121 pontos amostrais, espaçados de 50 m, analisados por meio da geoestatística, na profundidade de 0-0,2 m. Foram determinados o pH em água; P e K disponíveis; Ca, Mg e Al trocáveis, H + Al, B, Cu, Fe, Mn, Zn, S e MO. Calcularam-se a saturação por bases (V %, CTC total (T e soma de bases (SB. Os dados foram avaliados por estatística descritiva e pela análise de dependência espacial, com base no ajuste de semivariogramas. A maioria dos atributos apresentou coeficiente de variação (CV alto, sendo o maior encontrado para P, 73,51 %, e o menor, para pH em água, 5,96 %. A grande maioria dos atributos avaliados mostrou dependência espacial, a qual foi classificada como moderada e forte. A maioria dos dados se ajustou ao semivariograma de modelo gaussiano.The spatial variability of soil chemical attributes was evaluated in a commercial plantation under conventional soil tillage in Uberlândia, state of Minas Gerais, Brazil, in 2004. The sampling grid was the Santa Rosa Farm, where the soil is classified as very clayey Red Latosol (680 g kg-1 clay. The objective was to evaluate the spatial distribution and dependence of the soil chemical attributes in a plantation under conventional soil tillage. For this purpose, soil data were collected from a grid of 121 sampling points in the 0-0.2 m layer, spaced 50 m apart, and analyzed by means of geostatistics. The following soil attributes were assessed: soil p

  18. CARACTERIZAÇÃO FÍSICO-QUÍMICA, HISTOLÓGICA E VIABILIDADE DE BACTÉRIAS LÁCTICAS EM IOGURTES COM FRUTAS PHYSICO CHEMICAL, HISTOLOGYCAL AND VIABILITY OF LACTIC BACTERIA IN YOGURTS CONTAINING FRUIT

    Directory of Open Access Journals (Sweden)

    Maria Auxiliadora de Brito RODAS

    2001-12-01

    Full Text Available Foram analisadas 136 amostras de iogurtes com frutas de 8 marcas diferentes, adquiridas em supermercados da cidade de São Paulo, no período de 1997 a 1998, com a finalidade de caracterizá-los quanto aos parâmetros físico-químicos, identificação histológica das frutas adicionadas e viabilidade de bactérias lácticas viáveis. Nas avaliações foram utilizadas metodologias oficiais e recomendações. Quanto aos parâmetros físico-químicos, os resultados obtidos das variações médias e desvio padrão foram os seguintes: pH (4,01±0,11; substâncias voláteis (77,91%±1,85; resíduo mineral fixo (0,68%±0,10; lipídios (1,95%±0,65; proteínas (2,95%±0,46; carboidratos totais (15,89%±1,44 e valor calórico (93±9. Das 136 amostras analisadas, detectou-se fraude em 5,8% por não apresentarem as frutas indicadas na rotulagem, em 40,4% por apresentarem elementos histológicos de vegetais não discriminados pelos fabricantes e 44,1% por conterem fungos filamentosos, indicativo de utilização inadequada de matéria-prima. Quanto à viabilidade das bactérias lácticas, verificou-se a positividade da fermentação em até a diluição 10-7 em todas as amostras, mesmo naquelas que apresentavam apenas cocos Gram-positivos. Na contagem individual de estreptococos e de lactobacilos constituintes da microbiota láctica, 31,73% das amostras apresentaram quantidade igual ou superior a 10(7UFC/mL, limite mínimo indicado pela Recomendação Mercosul nº 31/97.One hundred and thirty six (136 samples of yogurts with fruits from eight different brands, acquired in supermarkets of São Paulo, in the period betwen 1997 and 1998, have been analysed aiming to characterise them for physico-chemical parameters, histologycal identification of added fruits and viability of lactic bacteria. On valuation official methodologies and recommendations were used . Concerning the physico-chemical parameters, the obtained results for medium verification and standard

  19. Energy-related pollutants in the environment: use of short-term tests for mutagenicity in the isolation and identification of biohazards.

    Science.gov (United States)

    Epler, J L; Larimer, F W; Rao, T K; Nix, C E; Ho, T

    1978-12-01

    In an effort to gather information on the potential genetic hazards of existing or proposed energy-generating or -conversion systems, we have begun a correlated analytical and genetic analysis of a number of technologies. The work is divided into two phases: one deals with known compounds expected to occur in the environment through energy production, conversion, or use; the other deals with actual samples from existing or experimental processes. To approach the problems of coping with and testing large numbers of compounds, we set up a form of the "tier system." Operating units utilizing Salmonella, Escherichia coli, yeast, human leukocytes, mammalian cells, and Drosophila have been initiated. Various liquid-liquid extraction methods and column chromatographic separations have been applied to crude products and effluents from oil-shale, coal-liquefaction, and coal-gasification processes. Mutagenicity of the various fractions is assayed by means of reversion of histidine-requiring auxotrophs of Salmonella typhimurium; comparative studies are carried out with the other genetic systems. In order to incorporate metabolic activation of these fractions and compounds, rat liver homogenates (S-9) are used in the various assays. Results implicate chemicals occurring in the basic (ether-soluble) and the neutral fractions as potential genetic hazards. Chemical constituents of these fractions (identified or predicted) were tested individually for their mutagenic activity. PMID:367762

  20. Mutagenic potency in Salmonella typhimurium of organic extracts of soil samples originating from urban, suburban, agricultural, forest and natural areas.

    Science.gov (United States)

    Courty, Benoit; Le Curieux, Frank; Belkessam, Laurence; Laboudigue, Agnès; Marzin, Daniel

    2008-05-31

    The purpose of the present work was to assess the mutagenic potency of soil samples presumably not contaminated by industrial wastes and discharges. A set of 51 soil samples was collected from areas considered as not contaminated by a known industrial activity: 11 urban samples (collected in cities), 15 suburban samples (collected in villages), 7 agricultural samples, and 18 forest or natural samples. Each soil sample was collected at the surface (0-5cm deep), dried, sieved (2mm), homogenized before organic extraction (dichloromethane/acetone 1/1 (v/v), 37 degrees C, 4h, soil/solvent ratio 1/2, m/v), solvent exchange to DMSO and sterilizing filtration. The micro-method adaptation of the standard bacterial mutagenicity test on Salmonella typhimurium strain TA98 was performed with and without a metabolic activation system (rat-liver homogenate S9), and thus detected the effect of pro-mutagens and direct mutagens, respectively. The use of a pre-incubation method increased the sensitivity of the assay. The results obtained showed a wide range of effect levels, from no effect to clear mutagenicity. In particular, the extract of all 11 urban soil samples demonstrated mutagenic activity, while the extracts of 10 of the 15 suburban samples showed mutagenicity. On the other hand, the extract of only one of the 7 agricultural samples studied induced mutations, and none of the 18 natural or forest-soil samples investigated produced mutagenic extracts. These findings seem to indicate the crucial influence of the diffuse pollution originating from different human activities on the mutagenic potency of urban soil samples. These findings make it possible to classify the soils according to their mutagenic potency. No clear correlation was found between the mutagenicity detected in soil extracts and the measured polycyclic aromatic hydrocarbon (PAH) content of the soils investigated. PMID:18440855

  1. An investigation into pharmaceutically relevant mutagenicity data and the influence on Ames predictive potential

    Directory of Open Access Journals (Sweden)

    McCarren Patrick

    2011-11-01

    Full Text Available Abstract Background In drug discovery, a positive Ames test for bacterial mutation presents a significant hurdle to advancing a drug to clinical trials. In a previous paper, we discussed success in predicting the genotoxicity of reagent-sized aryl-amines (ArNH2, a structure frequently found in marketed drugs and in drug discovery, using quantum mechanics calculations of the energy required to generate the DNA-reactive nitrenium intermediate (ArNH:+. In this paper we approach the question of what molecular descriptors could improve these predictions and whether external data sets are appropriate for further training. Results In trying to extend and improve this model beyond this quantum mechanical reaction energy, we faced considerable difficulty, which was surprising considering the long history and success of QSAR model development for this test. Other quantum mechanics descriptors were compared to this reaction energy including AM1 semi-empirical orbital energies, nitrenium formation with alternative leaving groups, nitrenium charge, and aryl-amine anion formation energy. Nitrenium formation energy, regardless of the starting species, was found to be the most useful single descriptor. External sets used in other QSAR investigations did not present the same difficulty using the same methods and descriptors. When considering all substructures rather than just aryl-amines, we also noted a significantly lower performance for the Novartis set. The performance gap between Novartis and external sets persists across different descriptors and learning methods. The profiles of the Novartis and external data are significantly different both in aryl-amines and considering all substructures. The Novartis and external data sets are easily separated in an unsupervised clustering using chemical fingerprints. The chemical differences are discussed and visualized using Kohonen Self-Organizing Maps trained on chemical fingerprints, mutagenic substructure

  2. Influence of caffeine on chromosome lesions induced by chemical mutagens and radiation. 2

    International Nuclear Information System (INIS)

    The modifying influence of caffeine on γ-ray induced chromosome lesions was studied by chromosome aberration anaysis. Caffeine was applied as a pre- and post-treatment agent following seed (G1) and root meristem (G2 and S) irradiation of C.capillaris. The frequency of chromosome aberrations induced in G1 was changed neither by post- nor by pre-treatment with caffeine. This fact proves the lack of caffeine modifying effect. Applied as a post-treatment agent caffeine enhances considerably the frequency of chromosome aberrations induced in root meristem cells. This is especially valid for G2 irradiated cells, while in S cells no synergistic effect was established between induced chromosome lesions and caffeine. The enhancement of chromosome aberration frequency produced in G2 shows a clearly manifested dependence on the time (moment) of caffeine application post irradiation. Most considerable enhancement was obtained following post-treatment with caffeine immediately after irradiation. In the following intervals - 15 and 30 min - it decreases progressively, while after 60, 180 and 300 min no enhancing effect is observed. The probable causes for the manifestation and the lack of synergistic effect between chromosome lesions induced in the various mitotic cycle phases and caffeine are discussed. (author)

  3. Two Simulated-Smog Atmospheres with Different Chemical Compositions Produce Contrasting Mutagenicity in Salmonella**

    Science.gov (United States)

    Ozone (O3), particulate matter (PM), and nitrogen dioxide (NO2) are criteria pollutants used to evaluate air quality. Using EPA’s Mobile Reaction Chamber (MRC), we generated 2 simulated-smog atmospheres (SSA-1 & SSA-2) with different concentrations of these criteria pol...

  4. Two Simulated-Smog Atmospheres with Different Chemical Compositions Produce Contrasting Mutagenicity in Salmonella.

    Science.gov (United States)

    Ozone (O3), particulate matter (PM), and nitrogen dioxide (NO2) are criteria pollutants used to evaluate air quality. Using a 14.3-m3 Teflon-lined smog chamber with 120 UV bulbs to simulate solar radiation, we generated 2 simulated-smog atmospheres (SSA-1 & SSA-2) with differ...

  5. Evaluation of winter rye (Secale cereale L.) and triticale after using physical and chemical mutagens

    International Nuclear Information System (INIS)

    Full text: It was decided to induce mutations separately in rye and triticale to obtain forms resistant to lodging. Seeds of rye cv. 'Dankowskie Zlote' and triticale cv. 'Lasko' were irradiated with fast neutrons and treated with MNH, rye cv. 'LAD 2T80' was treated with only MNH. The mutant selection was made in M3 and the progenies were evaluated with regard to plant height. In total, 226 changed forms were found, most of them shorter than the control. Some of them should be useful as a source of resistance to lodging. (author)

  6. Induction of cytoplasmic male sterility by gamma-ray and chemical mutagens in sugar beets

    International Nuclear Information System (INIS)

    Male sterile plants appeared in the population of N cytoplasm sugar beet strains, H-19 and H-2002, when their dry seeds were exposed to 50 kR gamma-ray, and the male sterility was maintained up to the M4 generation through the mother plants. Cytoplasmic inheritance was confirmed by the reciprocal crossings between plants with normal phenotype from gamma-strains (progeneis of the male mutants which transmitted male sterility through the mother plants) and H-19 or H-1001. The crossing experiments suggested that various kinds of cytoplasm were induced by gamma-ray irradiation, and that different nuclear genes were responsible for the respective cytoplasms. A specific relationship between the pollen restoring genes and the sterile cytoplasms was established, and was named ''one set of pollen restoring genes for one cytoplasm''. It is probable that the cytoplasmic mutation occurred in normal cytoplasm strains and the specific combination between the altered cytoplasm and the recessive nuclear gene produced male sterility. Ethyl methane sulphonate, ethidium bromide, acriflavine and streptomycin were also effective in inducing cytoplasmic mutation in sugar beets. (Kaihara, S.)

  7. Efeito da solarização e da adubação sobre artrópodes em solo cultivado com alface Solarization, organic and chemical fertilization combined effects on arthropods community in soil cultivated with lettuce

    Directory of Open Access Journals (Sweden)

    Marlene G da Silva

    2009-12-01

    Full Text Available Avaliou-se o efeito da solarização e da adubação química e orgânica na comunidade de artrópodes de solo na cultura da alface, cv. Verônica. Foram conduzidos dois experimentos, em blocos ao acaso, um com solarização e o outro sem solarização, em casa de vegetação, ambos com cinco tratamentos: adubação orgânica; nitrogênio na forma amoniacal (NH4; adubação com NPK; adubação orgânica + NPK; testemunha (sem adubação. No experimento solarizado, o solo foi coberto durante 132 dias com plástico transparente. Os artrópodes foram coletados por meio de armadilhas tipo alçapão em três épocas (antes da implantação do experimento, após a solarização e após a colheita. Foram utilizados índices faunísticos e de diversidade em cada experimento, tratamento e épocas. Os principais grupos coletados foram: Collembola (82,8%, Acari (7,1%, Hymenoptera (6,1% Coleoptera (1,3% e outros (2,7%. A classe Collembola foi a mais abundante, mesmo na colheita, quando a subordem Acari aumentou substancialmente. As espécies de Collembola predominantes foram: Proisotoma tenella (Reuter (34,3%; Seira atrolutea (Arlé (29%; Folsomides centralis (Denis (7,2%; Isotomurus sp. 161 (2,4% e Sminthurides sp. 98 (1,6%. Observou-se que a diversidade e abundância da comunidade de artrópodes não foi influenciada pela solarização ou adubação.To evaluate the effect of solarization, chemical and organic fertilization on soil arthropods community in lettuce, two blocks plots trials were carried out in Brasília, Brazil. One research was conducted with solarization and the other without solarization in a soil cultivated with lettuce, cv Veronica, under protected cultivation. Each trial consisted of five fertilization treatments: T1= organic fertilization; T2= amoniacal nitrogen (ammonium sulphate; T3= chemical fertilization; T4= organic and chemical fertilization; T5= control (without fertilization. In the solarized trial, the soil was covered for 132

  8. Mutagenicity tests of irradiated onions by Escherichia coli mutants in vitro

    International Nuclear Information System (INIS)

    Sprout inhibition of onion bulbs can be effectively accomplished by low doses of radiation. However, wholesomeness data on irradiated onions, particularly with respect to their mutagenic activity, are still insufficient for evaluation. The authors have therefore examined the mutagenic activity of irradiated onions in bacterial systems. Because onion bulbs contain a considerable amount of free amino acids, indicator strains were used carrying the marker for mutagenicity other than the amino acid requirement. The results on irradiated onions are described. Tests are used with solid and liquid media, assaying for the streptomycin (SM) dependence in a strain having a tetracycline (TC)-resistance factor, as well as DNA repair tests using two sets of indicator strains. (Auth.)

  9. AN INVESTIGATION OF MUTAGENIC ACTIVITIES OF SOME 9- SUBSTITUEDPHENANTHRENE DERIVATIVES WITH AMES / SALMONELLA / MICROSOME TEST

    Directory of Open Access Journals (Sweden)

    Mehtap KUTLU

    2011-02-01

    Full Text Available In this study, three different 9-Substituted phenantrene derivatives that were synthesized to be used as basic matter for drugs were tested for their mutagenic potency in strains TA 98 and TA 100 ofSalmonella typhimurium by using Ames test. Therefore, both strains were tested in the absence or presence of S9 metabolic activation, for five different doses of each test substances in two paralel independent experiments. In the absence of metabolic activation, while only one of the compounds were causing frame-shift mutations, two of them were determined to induce base pair substitution mutations. In the presence of metabolic activation, while two of the compounds were mutagenic for TA 98, base pair substitution mutations were not detected for any of the substances. Each of the compoundstested were found to be mutagenic for at least one strain in the presence or absence of S9.

  10. Mutagenicity test using Vibrio harveyi in the assessment of water quality from mussel farms.

    Science.gov (United States)

    Ruiz, Yolanda; Suárez, Pilar; Alonso, Ana; Longo, Elisa; San Juan, Fuencisla

    2013-05-15

    This work analyses the mutagenicity of seawater from mussel farms using the Vibrio harveyi mutagenicity test and its relationship with the accumulated pollutants and the development of gonadal neoplasia in mussels. Histological disorders identified as germinoma were observed in the gonad of Mytilus galloprovincialis during the period of study. The prevalence of this pathology is significantly correlated with certain levels of pollutants accumulated in mussels, mainly of PAHs and PCBs, whose toxic equivalents were calculated as EROD induction equivalency. The mutagenicity and toxicity of the water surrounding mussel's farms is clearly correlated with the pollutants accumulated and with the neoplasia prevalence in mussels. Such correlations are corroborated by a multivariate analysis. Our results conclude with the utility of V. harveyi test as an optimal and rapid method in the monitoring of the quality of the water from mussel farms and as a tool to control the risks of pollution on mussel production and its safety for human food. PMID:23510693

  11. Studies on induced mutation frequency in Catharanthus roseus (L.) G. Don by gamma rays and EMS individually and in combination

    International Nuclear Information System (INIS)

    Seeds of pink flowered (PF) and white flowered (WF) Catharanthus roseus were soaked in distilled water for 24 h and treated with gamma rays and 0.1% EMS separately and in combination. Six types of chlorophyll mutations, viz., xantha, albina, chlorina, viridis, maculata and tigrina were recovered to M2 generation of both forms. The frequency of chlorophyll mutations was found to be dependent on the dose, of gamma rays and duration of treatment with EMS. Higher frequency of chlorophyll mutations was noticed in PF, which is mutagenically more sensitive than WF. It was also noticed that the combination treatments of gamma rays and EMS enhanced the frequency of chlorophyll mutations

  12. Basic mechanisms for mutagenicity and carcinogenicity of environmental pollutants

    International Nuclear Information System (INIS)

    It is a difficult task to compare radiation with chemicals on the molecular level because there is only limited information on lesions which remain after DNA repair processes and which are responsible for late effects. Repair mechanisms, interaction of chemicals with DNA or DNA repair processes, as well as cellular model systems with and without excision repair are described. (author)

  13. Development and application of human cell lines engineered to metabolically activate structurally diverse environmental mutagens

    Science.gov (United States)

    Crespi, C. I.; Langenbach, Robert; Gonzalez, Frank J.; Gelboin, Harry V.; Penman, B. W.

    1993-03-01

    Cytochromes P450 are responsible for the mutagenic/carcinogenic activation of many environmental promutagens/procarcinogens. These enzymes are present at highest concentrations in liver in vivo but are markedly absent in tester organisms for most in vitro mutagenicity test systems. Two approaches have been used to supply needed metabolic activation, incorporation of an extracellular activating system, usually derived from a rodent liver and introduction of activating enzymes into the target cell. The latter approach appears to result in a more sensitive testing system because of the close proximity of the activating enzymes and the target DNA. Human cell lines have been developed which stably express human cytochromes P450 and other cDNAs which have been introduced individually or in combination. The resulting cell lines are exquisitely sensitive to exposure to promutagens and procarcinogens. Mutagenicity is measured at the hypoxanthine phosphoribosyl transferase (hprt) and thymidine kinase (tk) gene loci. The most versatile cell line, designated MCL-5, stably express five cDNAs encoding all of the human hepatic P450s known to be principally responsible for known human procarcinogen activation. The induction of mutation is observed in MCL-5 cells upon exposure to ng/ml levels of model compounds such as nitrosamines, aflatoxin B1 and benzo(a)pyrene. A lower volume mutagenicity assay has been developed for use with samples available in limited amounts. Human lymphoblast mutation assays have been used to screen for mutagenic activity sediment samples from a polluted watershed. Two sediment samples were found to have mutagenic activity to human lymphoblasts.

  14. Use of four short-term tests to evaluate the mutagenicity of municipal water

    Energy Technology Data Exchange (ETDEWEB)

    DeMarini, D.M. (Illinois State Univ., Normal); Plewa, M.J.; Brockman, H.E.

    1982-01-01

    Some ways in which four short-term tests may be used to evaluate the mutagenicity of drinking water were explored by testing raw and treated water from Lake Bloomington, which serves the town of Bloomington, Illinois (population, 44,000). The water was collected from February 1976 to October 1977 and was concentrated by evaporation or by use of XAD-2 resin. The water was tested for the ability to induce reverse mutation in a prokaryote, Salmonella typhimurium; forward mutation in a mold, Neurospora crassa; mitotic gene conversion in a yeast, Sccharomyces cerevisiae; and reverse mutation in maize, Zea mays. Because of the large number of water samples (54) and the limited amounts of the samples, it was not possible to test all samples in all four tests by all the protocols. Thus, the sensitivities of the four tests to potential mutagens in the water samples could not be rigorously compared. However, the results do show that lake and tap water samples collected during 1976 were toxic but not mutagenic in N. crassa and neither toxic nor genotoxic in S. cerevisiae; lake water collected during 1977 was mutagenic in one line of Z. mays and slightly mutagenic in S. typhimurium strain TA1536 in the presence of rat liver S9. The results suggest that tests that detect a variety of genetic end points should be used when testing complex mixtures such as drinking water. The advantages and disadvantages of the tests and protocols are discussed in terms of their applicability to the study of the mutagenicity of drinking water.

  15. Intrinsic mutagenic properties of 5-chlorocytosine: A mechanistic connection between chronic inflammation and cancer.

    Science.gov (United States)

    Fedeles, Bogdan I; Freudenthal, Bret D; Yau, Emily; Singh, Vipender; Chang, Shiou-chi; Li, Deyu; Delaney, James C; Wilson, Samuel H; Essigmann, John M

    2015-08-18

    During chronic inflammation, neutrophil-secreted hypochlorous acid can damage nearby cells inducing the genomic accumulation of 5-chlorocytosine (5ClC), a known inflammation biomarker. Although 5ClC has been shown to promote epigenetic changes, it has been unknown heretofore if 5ClC directly perpetrates a mutagenic outcome within the cell. The present work shows that 5ClC is intrinsically mutagenic, both in vitro and, at a level of a single molecule per cell, in vivo. Using biochemical and genetic approaches, we have quantified the mutagenic and toxic properties of 5ClC, showing that this lesion caused C→T transitions at frequencies ranging from 3-9% depending on the polymerase traversing the lesion. X-ray crystallographic studies provided a molecular basis for the mutagenicity of 5ClC; a snapshot of human polymerase β replicating across a primed 5ClC-containing template uncovered 5ClC engaged in a nascent base pair with an incoming dATP analog. Accommodation of the chlorine substituent in the template major groove enabled a unique interaction between 5ClC and the incoming dATP, which would facilitate mutagenic lesion bypass. The type of mutation induced by 5ClC, the C→T transition, has been previously shown to occur in substantial amounts both in tissues under inflammatory stress and in the genomes of many inflammation-associated cancers. In fact, many sequence-specific mutational signatures uncovered in sequenced cancer genomes feature C→T mutations. Therefore, the mutagenic ability of 5ClC documented in the present study may constitute a direct functional link between chronic inflammation and the genetic changes that enable and promote malignant transformation. PMID:26243878

  16. Mushroom Shiitake, is it mutagenic or antimutagenic agent? Shiitake, um cogumelo mutagênico ou antimutagênico?

    Directory of Open Access Journals (Sweden)

    Ilce Mara de Syllos Cólus

    2001-11-01

    Full Text Available Mushrooms have been consumed by eastern populations due to their edible and medicinal properties, for a long time. Over the years, there has also been a gradual increase in the consumption of mushrooms in the western countries, including Brazil. However, the benefits or harms that these mushrooms may cause to the human health are still uncertain. Shiitake (Lentinula edodes (Berk. Pegler is one of the most appreciated fungi in the Brazilian cuisine and its functional and nutritional properties have been extensively studied. Other characteristics of this basidiomycet have also been reported, including its antitumoral, antiviral, bactericidal properties, as well as being a homeostasis and biorhythm regulator. However, studies regarding its mutagenic and antimutagenic potential in prokaryotic and eukaryotic organisms are scarce. Knowledge of these biological properties is essential before a product can be recommended as food and/or drug. Therefore, its indication as a beneficial product to human health is premature since it is necessary to broaden studies on the mutagenic and antimutagenic activities of the mushroom Shiitake or of its active compounds.   Desde a antigüidade, os cogumelos vêm sendo consumidos pelos povos orientais devido às suas  propriedades medicinais e comestíveis e ao longo dos anos tem-se observado um aumento gradativo no consumo também nos países ocidentais, inclusive o Brasil. No entanto, ainda há dúvidas quanto aos benefícios ou prejuízos à saúde, decorrentes do uso destes cogumelos pelas populações humanas. Entre os cogumelos muito apreciados na culinária brasileira, destaca-se o Shiitake (Lentinula edodes (Berk. Pegler, um dos fungos comestíveis mais estudados quanto às suas propriedades funcionais e nutricionais. Já se tem conhecimento sobre várias atividades deste basidiomiceto superior, como antitumoral, antiviral, bactericida, regulador da homeostase e do biorritmo. No entanto, poucos trabalhos

  17. Atributos químicos e produção de milho em um latossolo vermelho eutroférrico tratado com lodo de esgoto Soil chemical properties and corn production in a sewage sludge-amended soil

    Directory of Open Access Journals (Sweden)

    M. V. Galdos

    2004-06-01

    Full Text Available A utilização de lodo de esgoto como fertilizante orgânico ou condicionador do solo, tem-se tornado cada vez mais atraente, pelos altos custos e impactos ambientais relacionados com os demais métodos de disposição, pela presença de nutrientes vegetais e matéria orgânica no lodo e pela necessidade de redução de custos na agricultura. No entanto, em sua composição, pode conter metais pesados, microrganismos patogênicos e compostos orgânicos tóxicos. Visando estudar os efeitos da aplicação de lodo de esgoto em áreas agrícolas, foi realizado um experimento, com duração de dois anos agrícolas, para determinar alterações em propriedades químicas do solo, principalmente sobre os teores de P, Cu, Ni e Zn em um Latossolo Vermelho eutroférrico, de textura argilosa, cultivado com milho. O experimento consistiu na aplicação de lodo de esgoto em duas doses comparadas a uma testemunha com adubação química. Para análise de rotina das propriedades químicas e do teor de Ni do solo, foram coletadas amostras nas profundidades de 0-0,05; 0,05-0,10 e 0,10-0,20 m após a colheita do milho no primeiro e no segundo ano. Foi feita, também, uma extração seqüencial de P em amostras da camada de 0-0,05 m, na seguinte ordem: CaCl2 (P biologicamente mais disponível; NaHCO3 (P disponível; NaOH (P ligado aos óxidos de Fe e Al; HCl (P ligado a Ca e digestão nítrico-perclórica (P residual. A produção do milho foi maior nos tratamentos com aplicação do lodo. Os teores disponíveis de P no solo onde foi aplicado lodo foram semelhantes aos do tratamento sem lodo e com adubo químico. Entretanto, a aplicação do lodo aumentou as frações lábeis e moderadamente lábeis do P na camada superficial. Os dados indicaram, por outro lado, aumento dos teores de Cu, Ni e Zn no solo e de Zn na planta. Dessa forma, faz-se necessário constante monitoramento do solo onde o lodo de esgoto é aplicado para o controle adequado dos teores de metais

  18. Chemical and biological stability of solvent refined coal liquids

    Energy Technology Data Exchange (ETDEWEB)

    Wright, C.W.; Weimer, W.C.

    1984-01-01

    Stability studies performed on seventeen SRC samples in boiling point from ambient to 850/sup 0/F showed that the major chemical composition of the materials as monitored by high resolution gas chromatography did not change under the storage conditions of the repository, which were 4/sup 0/C, in inert containers, under a nitrogen atmosphere, in the dark. Samples were monitored after two years of storage. It was also found from microbial mutagenicity studies that after four years in the repository there was no significant change in the biological activity of any of the SRC materials. Samples stored under various parameters of air versus nitrogen atmosphere and ambient light versus darkness at room temperature and -20/sup 0/C for one year showed there was no significant differences in the chemical composition of any of the samples. There was evidence, however, that trace components such as amino-PAH degraded at room temperature, in the light, under an air atmosphere since the microbial mutagenicity of samples stored under these conditions for one year decreased significantly. Both the chemical composition and mutagenicity of FOB samples changed when stored diluted in methylene chloride, in the light, under an air atmosphere at room temperature. After one year of storage under these conditions, the microbial mutagenicity was eliminated. Storage of SRC-II FOB at increased temperatures of 60/sup 0/C and 100/sup 0/C showed significant changes in chemical composition due to volatility effects. The microbial mutagenicity of the FOB samples was completely eliminated after storage at 60/sup 0/C for 32 weeks and 100/sup 0/C for 26 weeks. It appears that the amino-PAH and phenolic materials are the most susceptible components to degradation in the complex SRC materials. 23 references, 29 figures, 50 tables.

  19. 2D/3D-QSAR comparative study on mutagenicity of nitroaromatics

    Institute of Scientific and Technical Information of China (English)

    WANG Xiaodong; LIN Zhifen; YIN Daqiang; LIU Shushen; WANG Liansheng

    2005-01-01

    Nitroaromatics are typical toxic organic pollutants and are ubiquitous in environment with diverse structures. They are widely used in many industries and formed during many natural and anthropogenic processes. Most of these pollutants are potentially carcinogenic and the assessment and prediction of the mutagenicity of nitroaromatics are of great interest. In this paper the structure-mutagenicity relationships of 219 nitroaromatics are investigated by molecular orbital theory based classic structure-activity relationships and comparative molecular field analysis (CoMFA). A comparison is undertaken in respect of the interpretation of mechanism and predictive ability for these two categories of QSAR approaches and highly predictive QSAR models have been developed.

  20. Mutagenicity testing with transgenic mice. Part I: Comparison with the mouse bone marrow micronucleus test

    OpenAIRE

    Wahnschaffe U; Bitsch A; Kielhorn J; Mangelsdorf I

    2005-01-01

    Abstract As part of a larger literature study on transgenic animals in mutagenicity testing, test results from the transgenic mutagenicity assays (lacI model; commercially available as the Big Blue® mouse, and the lacZ model; commercially available as the Muta™Mouse), were compared with the results on the same substances in the more traditional mouse bone marrow micronucleus test. 39 substances were found which had been tested in the micronucleus assay and in the above transgenic mouse system...

  1. Fluoro-A-85380 demonstrated no mutagenic properties in in vivo rat micronucleus and Ames tests

    International Nuclear Information System (INIS)

    The potential mutagenic properties (micronucleus and the Ames tests) of fluoro-A-85380 (2-fluoro-3-[2(S)-2-azetidinylmethoxy]pyridine) were evaluated as a mandatory pre-clinical step. No statistically significant increase in the frequency of micronucleated polychromatic erythrocytes was found in animals treated at any dose tested. No biologically significant increase in the mean number of revertants was noted in all the Salmonella typhimurium strains tested with fluoro-A-85380. Therefore, fluoro-A-85380 demonstrated no mutagenic properties using these two tests

  2. Controle químico de Anastrepha fraterculus (Wied. (Diptera: Tephritidae em laboratório Chemical control of Anastrepha fraterculus (Wied. (Diptera: Tephritidae in laboratory

    Directory of Open Access Journals (Sweden)

    Priscila Lang Scoz

    2004-12-01

    Full Text Available O efeito de quatro novos grupos químicos de inseticidas incluindo avermectina (benzoato de emamectina, éter piretróide (etofemprox, neoniconitnóide (imidacloprid, thiacloprid e thiamethoxan e naturalyte (spinosad foram avaliados em laboratório (25 ± 3ºC, umidade relativa de 70 ± 10% e fotofase de 12 horas, visando ao controle de adultos e ovos/larvas de Anastrepha fraterculus comparando-os com os fosforados fenthion e thrichlorphon. O benzoato de emamectina não foi eficiente no controle de adultos de A. fraterculus via contato e ingestão. O etofenprox, imidacloprid, spinosad e thiamethoxan foram eficientes no controle de adultos de A. fraterculus via contato e ingestão, proporcionando maior mortalidade via ingestão. Os novos inseticidas não provocaram mortalidade significativa de ovos/larvas de A. fraterculus localizados no interior de maçãs, enquanto que os fosforados fenthion e thrichlorphon resultaram em 100% de mortalidade das fases imaturas e adultos. Os novos inseticidas apresentam potencial para uso nas iscas tóxicas, substituindo os fosforados para o controle de adultos.The South American Fruit Fly, Anastrepha fraterculus is one of the most important pest of temperate fruit crops. The effect of four new inseticide groups to replace organophosphate compounds for A. fraterculus control was evaluated under laboratory conditions (25 ± 3ºC, relative humity of 70 ± 10% and 12:12 L:D. Emamectin benzoate, etofenprox, imidacloprid, spinosad, thiacloprid and thiamethoxan were evaluated to control adults by contact and ingestion and against eggs/larvae inside apple fruits compared with fenthion and thrichlorphon. Emamectin benzoate was not efficient to control adults of A. fraterculus by contact and ingestion. Etofenprox, imidacloprid, spinosad and thiamethoxan were efficient to control adults by contact and ingestion being more toxic by ingestion. No new insecticide controlled eggs/larvae inside apple fruit while organophosphate

  3. Chemical characterization of polymeric surge arresters and insulators used in high voltage lines; Caracterizacao quimica de para-raios e isoladores utilizados em linhas de alta tensao

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira Junior, Joao B. de; Castro Junior, Joao B. de; Silva, Maria Elisa S.R. e; Freitas, Roberto F.S.; Sousa, Ricardo G. de [Lab. de Ciencia e Tecnologia de Polimeros, Dept. de Engenharia Quimica da EE.UFMG, Belo Horizonte, MG (Brazil); Souza, Breno P.G. de [CEMIG Distribuicao S/A, Belo Horizonte, MG (Brazil)], e-mail: bpgsouza@gmail.com

    2011-07-01

    In the last two decades, traditional porcelain and glass surge arresters and insulators, used in the electrical system, have been replaced by polymeric materials. For this type of application it is recommended that these devices have a high resistance to environmental stresses and impacts without suffering degradation process. Considering the environmental conditions these polymers are exposed to, when used for this purpose, studies of their chemical composition are needed. In this work, polymeric materials present in surge arresters and insulators used in electrical system were chemically characterized by Fourier Transform Infrared Spectroscopy (FTIR). FTIR analysis showed that the samples tested are made of poly(dimethylsiloxane) - PDMS (silicone) and additives such as alumina trihydrated [Al (OH)3] and calcium carbonate. (author)

  4. Treatment of chemical waste piassava for application in polymeric composites; Tratamento quimico do residuo de piacava para aplicacao em compositos polimericos

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, C.S.; Fiuza, R.P.; Guimaraes, D.H.; Carvalho, G.G.P.; Carvalho, R.F.; Jose, N.M., E-mail: cleidienesm@gmail.co [Universidade Federal da Bahia (GECIM/UFBA), Salvador, BA (Brazil). Inst. de Quimica. Grupo de Energia e Ciencias dos Materiais

    2010-07-01

    Piassava fibers were investigated with the aim of adding new business value. The surface of the fibers were treated with NaOH and H{sub 2}SO{sub 4} for 1 h at room temperature. The samples were characterized by FTIR, TGA, DSC, chemical composition, XRD, SEM and tensile tests. The micrographs of the fibers showed that treatment with NaOH cleaned the fiber surface of a large amount of impurities and cause fibrillation. Chemical analysis, using the Van Soest method, showed that the palm fiber is a fiber rich in lignin, as evidenced by their brown color and with alkali treatment there was partial removal of hemicellulose and lignin, increasing the crystallinity index of the fiber, observed by XRD. The acid treatment caused no significant changes in the properties of the fiber. Therefore, the mercerisation was efficient in the fiber of palm fiber, improving their properties, enabling thus their use as reinforcement in polymer composites. (author)

  5. Surfactina: propriedades químicas, tecnológicas e funcionais para aplicações em alimentos Surfactin: chemical, technological and functional properties for food applications

    OpenAIRE

    Francisco Fábio Cavalcante Barros; Cedenir Pereira de Quadros; Mário Roberto Maróstica Júnior; Gláucia Maria Pastore

    2007-01-01

    Surfactin, a lipopeptide produced by strains of Bacillus subtilis, has been proved to be a suitable biosurfactant in several applications. For many years, it has been investigated mainly for oil recovery and environmental usage. Its chemical, technological and functional characteristics turn surfactin into an attractive compound for several utilizations. In this review we emphasize some aspects of surfactin as a new food ingredient and its potential pharmaceutical and health applications.

  6. Surfactina: propriedades químicas, tecnológicas e funcionais para aplicações em alimentos Surfactin: chemical, technological and functional properties for food applications

    Directory of Open Access Journals (Sweden)

    Francisco Fábio Cavalcante Barros

    2007-04-01

    Full Text Available Surfactin, a lipopeptide produced by strains of Bacillus subtilis, has been proved to be a suitable biosurfactant in several applications. For many years, it has been investigated mainly for oil recovery and environmental usage. Its chemical, technological and functional characteristics turn surfactin into an attractive compound for several utilizations. In this review we emphasize some aspects of surfactin as a new food ingredient and its potential pharmaceutical and health applications.

  7. A geração química de vapor em espectrometria atômica Chemical vapor generation in atomic spectrometry

    OpenAIRE

    Iracema Takase; Hugo Borges Pereira; Aderval S. Luna; Patrícia Grinberg; Reinaldo Calixto de Campos

    2002-01-01

    The historical development of atomic spectrometry techniques based on chemical vapor generation by both batch and flow injection sampling formats is presented. Detection via atomic absorption spectrometry (AAS), microwave induced plasma optical emission spectrometry (MIP-OES), inductively coupled plasma optical emission spectrometry (ICP-OES) , inductively coupled plasma mass spectrometry (ICP-MS) and furnace atomic nonthermal excitation spectrometry (FANES) are considered. Hydride generation...

  8. Alterações físico-químicas em morangos (Fragaria anassa Duch. irradiados e armazenados Physical-chemical alterations in irradiated and stored strawberries (Fragaria anassa Duch.

    Directory of Open Access Journals (Sweden)

    Iara Luiza Tassim Françoso

    2008-09-01

    Full Text Available Os morangos possuem curta vida útil pós-colheita devido à rápida deterioração causada por fungos, mesmo quando armazenados sob refrigeração. O presente trabalho teve por objetivo avaliar as alterações físico-químicas em morangos irradiados. Morangos (Fragaria anassa Duch. da variedade Sweet Charles, colhidos na região de Valinhos-SP, foram irradiados um dia após a colheita em fonte de 60Co, nas doses de 0,5; 1,0; 1,5 e 2,0 kGy e controle, armazenados sob refrigeração (4 °C por 1, 8, 15, 22 e 29 dias. Para a caracterização dos frutos foi realizada a composição química. Os frutos foram avaliados quanto aos teores de sólidos solúveis totais (°Brix, acidez total titulável, pH, pectina total e solúvel nos diferentes tempos. A irradiação não provocou alterações em nenhum dos parâmetros analisados, pois não foram verificadas diferenças entre as amostras irradiadas e a controle, já entre os períodos de armazenamento ocorreram diferenças em todos os parâmetros avaliados.Strawberries have a short postharvest life due to the rapid spoilage caused by fungal infections, even when stored under refrigeration. The present research had the objective of evaluating the physical and chemical alterations in irradiated strawberries. Strawberries (Fragaria anassa Duch. of the Sweet Charles variety, harvested in the region of Valinhos-SP, Brazil, were irradiated one day after harvest with a source of 60Co in the doses of 0.5; 1.0; 1.5 and 2.0 kGy and control, stored under refrigeration (4 °C for 1, 8, 15, 22 and 29 days. The control sample was not submitted to irradiation. For the characterization of the fruits, the chemical composition was determined. The fruits were evaluated as to total soluble solid content (°Brix, total titrimetric acidity, pH and total and soluble pectin at the different time periods. Irradiation did not cause alterations in any of the parameters analyzed between irradiated samples and the control, but the

  9. Características físico-químicas de derivados proteicos de soja em dietas extrusadas para cães Physical-chemical characteristics of soy-protein derived in extruded diets for dogs

    Directory of Open Access Journals (Sweden)

    Ananda Portella Félix

    2010-12-01

    Full Text Available A soja é um grão rico em proteínas e lipídeos, a partir do qual se obtém diversos derivados proteicos de alto valor nutricional. Em virtude disso, realizou-se este estudo com o objetivo de analisar a composição bromatológica de derivados proteicos de soja, bem como as características físico-químicas dos extrusados formados a partir destes. Foram formuladas seis dietas para cães, uma referência e cinco contendo 30% de derivados de soja (farinha desengordurada - FDS, micronizada, farelo, grão integral e grão tostado, as quais foram extrusadas. Os derivados de soja e as dietas foram submetidos a análises bromatológicas e de qualidade do processamento. A FDS e o farelo de soja apresentaram os maiores teores de proteína bruta, enquanto os grãos de soja integral e tostado e a soja micronizada apresentaram os maiores teores de extrato etéreo em hidrólise ácida e energia bruta. A soja grão integral apresentou a maior atividade ureásica e inibitória de tripsina, mesmo após a extrusão da dieta. Os extrusados contendo as sojas grão e micronizada apresentaram as maiores densidades e as menores durezas, índice de absorção de água e grau de gelatinização do amido. Derivados proteicos de soja apresentam alto valor nutricional, entretanto, devem ser termicamente processados antes da extrusão para completa inativação do inibidor de tripsina. Além do mais, derivados contendo alto teor de lipídeos, como soja grão e micronizada, quando adicionados em 30% da fórmula, comprometem a qualidade do extrusado.Soy is a grain rich in protein and lipids, from which its obtained different protein products of high nutritional value. This study aimed to analyze the chemical composition of different soy-protein products, as well as the characteristics of the extruded produced. Six diets were formulated, a reference and five with 30% soy product (defatted soy flour - DSF, micronized, soybean meal, crude grain or toasted grain, which were

  10. Qualidade química da água residual da criação de peixes para cultivo de alface em hidroponia Chemical quality of residual water from fish breeding tanks for cultivation of hydroponic lettuce

    Directory of Open Access Journals (Sweden)

    Glauco E. P. Cortez

    2009-08-01

    Full Text Available Com o objetivo de avaliar a associação do cultivo de alface em hidroponia com utilização dos resíduos do sistema de criação intensiva de peixe, desenvolveu-se um trabalho no Centro de Aqüicultura, na FCAV-UNESP, Campus de Jaboticabal, SP. A integração foi projetada para que a água circulasse de maneira fechada entre os sistemas, passando pelos tanques de criação de peixes, por um decantador, para remo��ão dos resíduos por um reservatório para conversão biológica da amônia em nitrato e pelo sistema hidropônico, retornando aos tanques de criação dos peixes. Foram avaliadas três cultivares de alface, que constituíram os tratamentos com quatro repetições. Os resultados da análise química da água residual da criação indicaram a presença da maioria dos nutrientes minerais necessários ao desenvolvimento vegetal, em concentração próxima aos valores encontrados em soluções nutritivas utilizadas para o cultivo da alface em hidroponia, exceto potássio e magnésio. A baixa concentração de magnésio na água não impediu o desenvolvimento da alface; entretanto, as plantas indicaram sintomas visuais de deficiência deste nutriente. Não houve diferenças entre as cultivares quanto à produtividade e ao peso fresco de plantas.Aiming to evaluate the association of hydroponic lettuce cultivation with residues from a fish intensive breeding system, a project was carried out in the Aquaculture Center in the FCAV-UNESP at Jaboticabal, SP, Brazil. A closed system was designed in order to allow the water to circulate through the fish tanks, a clarifier tank for removal of residues, a reservoir for biological conversion of ammonia into nitrate, and the hydroponic system. After this process, water returned back to the fish tanks. Three varieties of lettuce, constituting the treatments with four repetitions were evaluated. The results of the chemical analysis of the residual water from the fish tanks indicated the presence of

  11. Rendimentos do processamento e composição química de filés de surubim cultivado em tanques-rede Processing yield and chemical composition of fillets of surubim reared in net cages

    Directory of Open Access Journals (Sweden)

    Denilson Burkert

    2008-07-01

    Full Text Available Neste trabalho foi avaliado o efeito da utilização de três rações comerciais indicadas para alimentação de peixes carnívoros sobre os rendimentos do processamento e a composição de filés de surubim (Pseudoplatystoma sp cultivados em tanques-rede. Os surubins foram cultivados em tanques-rede durante um ano, período em que foram alimentados com três rações contendo 43,5; 42,6 e 42,1% de proteína bruta; 6,3; 8,7 e 4,5% de extrato etéreo e 5.078; 4.931 e 4.994 kcal de energia bruta, respectivamente, em um delineamento inteiramente casualizado, com três tratamentos e três repetições. Em cada tanque, foram selecionados cinco peixes com pesos entre 1.280 e 1.340 g, que foram insensibilizados e sacrificados para determinação dos rendimentos de carcaça com e sem pele, cabeça, filé total, lateral e abdominal, fígado, pele e esqueleto. Amostras de filés laterais e abdominais foram tomadas para análises bromatológicas. Foram avaliados 22 fêmeas e 23 machos, no entanto, não foi observada diferença entre os sexos quanto aos principais rendimentos de processamento. Os rendimentos de filé abdominal e de resíduos com e sem pele diferiram entre as rações. A composição bromatológica dos filés (lateral e abdominal não foi influenciada pelas rações e não diferiu entre os cortes. A utilização de rações comerciais no cultivo não acarretou mudanças na quantidade e na qualidade dos principais cortes do surubim.The objective of this work was to evaluate the effect of three commercial feeds recommended for carnivorous fish on processing yield and fillet composition of surubim (Pseudoplatystoma sp raised in net cages. The surubim fishes were raised in net cages during one year, when were fed three diets with 43.5, 42.6, and 42.1% crude protein; 6.3, 8.7, and 4.5% ether extract and 5,078, 4,931, and 4,994 kcal of gross energy, respectively. Fishes were allotted to a completely randomized design, with three treatments and

  12. EMS induced karyomorphological variations in Cichorium intybus L.

    Directory of Open Access Journals (Sweden)

    Iram Fatma Jafri *, Ainul Haq Khan, Mohd Gulfishan And M.Y.K. Ansari

    2011-12-01

    Full Text Available Cytogenetical investigations were carried out in Cichorium intybus plants derived from EMS treated seeds at 0.1, 0.2, 0.3, 0.4 and0.5% concentrations. The plants in the treated populations showed varying degrees of meiotic irregularities almost at allmutagenic concentrations. Variations in some parameters such as seed germination, pollen fertility, days to flowering, days tomaturity, number of leaves per plant, leaf length, plant height, and chromosome behavior have been studied in M1 generation. Apositive correlation between increasing concentrations of mutagen and chromosomal aberration has been observed

  13. CHEMICAL CONTROL OF Tetranychus urticae (KOCH, 1836 BORDEAUX & DOSSE 1963 IN TOMATO CONTROLE QUÍMICO AO ÁCARO Tetranychus urticae (KOCH, 1836 BORDEAUX & DOSSE 1963, EM TOMATEIROS

    Directory of Open Access Journals (Sweden)

    Antônio Lopes da Silva

    2007-09-01

    Full Text Available

    An experiment to test chemical control of the mite Tetranychus urticae (Koch 1836 Bordeaux & Dosse 1963, was carried out tomato plants, at the college of Agronomy and Veterinary Medicine of the University of Goiás. The following insecticides were tested: Diazinon E-60 (Thiophosphate-0-0-diethyl- -2-isopropyl-4-methyl-pirimidyl at 0.06%; Folidol 60 (Thiophosphate-0-0-dimethyl-0-p-nitrophenil at 0.06%; Kurnulus (80% coloidal sulfur at 0.03%; Metasystox-i (Thiophosphate-0-0-dimethyl-0-3-methylmercapto-ethyl at 0.025%; Lebaycid-50 (Thiophosphate-0-0-dimethyl-S-ethyl- ercaptoethyl at 0.065% and granulated Disyston at 2.5% (Dithiophosphate-0-0-Diethyl-S-2 (Thioethyl - ethyl at 2.5 g/plant. Disyston, Metasystox-i, Lebaycid and Folidol-60, were statistically superior to the others in controlling the mite. The highest percentage of control was reachead by Disyston. Next in order came Metasystox-i, Lebaycid-50, and Folidol-60. Diazinon-E-60 and sulfur cannot be recommended for controlling Tetranychus urticae.

    KEY-WORDS: Lycopersicon esculentum; acaricide.

    Realizou-se na Escola de Agronomia e Veterinária - Universidade Federal de Goiás, um ensaio de combate químico ao ácaro rajado Tetranychus urticae (Koch 1836 Bordeaux & Dosse 1963, sobre tomateiro. Os tratamentos realizados foram os seguintes: Diazinon-E-60 a 0,060% Folidol-60 a 0,060%, Kumulus (com 80% de enxofre coloidal a 0,30%, Metasystox “i” a 0,025%, Lebaycid-50 a 0,065% e Disyston 2,5% a 2,5g/pé. A análise estatística indicou que, dos produtos testados, Disyston, Metasystox, Lebaycid e Folidol foram os mais eficientes contra o ácaro rajado em tomateiro. A análise percentual mostrou que o Disyston superou todos os outros (Quadro IV, vindo em ordem decrescente o Metasystox, Lebaycid e Folidol. Os demais inseticidas

  14. Effect of explant treatment with gamma-rays, EMS and SA on somatic tissue culture in rice

    International Nuclear Information System (INIS)

    Dry seeds of two rice cultivars were treated with gamma-rays (0-40 kR), EMS (0-0.4M) and SA (0-4mM) and then inoculated in N6 medium containing 0.5mg/l 2,4-D, 4mg/l NAA and 2mg/l 6-BA to induce callus.Growth and differentiation' of the callus treated rice seeds were observed in subsequent subcultures.Decreased callus induction percentages were observed in treatments with gamma-rays, EMS and SA, but the mutagenic treatments did not reduce the mean weight of primary calli except that the seeds were irradiated with gamma-rays.Moreover, up to 3-fold increase in callus growth rate was shown after treatments of the three mu-tagenic agents.Higher proportion of calli with green spots or small shoots was also seen in one cultivar treated with the three mutagens.Signif icant increase in plant regeneration capacity was found in 60-day-old calli derived from gamma-rays or EMS-treated rice seeds in one cultivar.The results suggested that the following dose or concentration can be used for explant treatment in indica rice before culture, gamma-rays.5-10kR, EMS.<0.2M and SA:2-4mM

  15. Controle químico da microflora oral em pacientes fissurados labiopalatais durante o tratamento ortodôntico-cirúrgico: estudo piloto Oral microflora chemical control in cleft lip and palate patients during orthodontic-surgical treatment pilot study

    Directory of Open Access Journals (Sweden)

    Alcion Alves Silva

    2004-06-01

    Full Text Available O risco de infecção é uma preocupação durante o tratamento ortodôntico-cirúrgico, principalmente em pacientes como os fissurados lábio palatais, pois as condições anatômicas e oclusais contribuem para a proliferação microbiana. Métodos manuais para o controle da microflora oral são muitas vezes insuficientes para a obtenção de uma higiene satisfatória. Técnicas alternativas, como agentes químicos, têm sido propostas como procedimentos auxiliares. O objetivo deste estudo foi comparar a eficiência de dois agentes químicos como auxiliares no controle da microflora, em pacientes ortodôntico-cirúrgicos (fissurados lábio-palatais. O delineamento da pesquisa foi um estudo exploratório, microbiológico (piloto, duplo-cego, para testar os seguintes agentes químicos: triclosan 0,03% (Plax-Colgate Palmolive e clorexidina digluconada 0,12% (Duplak-Herpro/Dentsply. A amostra foi composta por 30 voluntários divididos em 3 grupos: Grupo I (controle, Grupo II (teste _ clorexidina digluconada 0,12% e Grupo III (teste _ triclosan 0,03%. Todos os grupos receberam orientação de higiene bucal prévia à primeira coleta de material. Depois de sete dias de uso dos agentes químicos uma segunda coleta foi realizada e o uso do agente foi suspenso. Uma terceira coleta foi realizada após sete dias a partir da suspensão do agente. A técnica semiquantitativa proposta por Pilonetto e Pilonetto20 foi adotada para avaliar o crescimento de colônias bacterianas. Os resultados mostraram diferenças entre a eficácia dos agentes químicos, sendo que o Grupo II mostrou uma redução mais significativa da microflora quando comparado ao grupo III.The infection risk is a preoccupation during orthodontic-surgical treatment, mainly at cleft lip and palate patients, for their anatomical and occlusal condictions, that contributs to oral microflora proliferation. Manual methods for microflora control are frequently unsuccessful to obtain a sctisfactory

  16. Organic matter in four Brazilian soil types: chemical composition and atrazine sorption; Materia organica em quatro tipos de solos brasileiros: composicao quimica e sorcao de atrazina

    Energy Technology Data Exchange (ETDEWEB)

    Dick, Deborah Pinheiro [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Inst. de Quimica. Dept. de Fisico-quimica; Martinazzo, Rosane [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Faculdade de Agronomia. Dept. de Solos; Knicker, Heike [Technische Universitaet Muenchen, Freising (Germany). Lehrstuhl fuer Bodenkunde; Almeida, Paulo Sergio Gois [Centro de Ensino Superior de Rondonopolis, MT (Brazil)

    2010-07-01

    Soil organic matter is the main sorptive soil compartment for atrazine in soils, followed in a minor scale by the inorganic fraction. In this study, the soil organic matter quality and atrazine sorption were investigated in four different soil types. The pedogenic environment affected the humification and therefore the chemical composition of the organic matter. The organic matter contribution to atrazine sorption was larger (60-83%) than that of the inorganic fraction. The organic matter capacity in retaining the herbicide was favoured by a higher decomposition degree and a smaller carboxylic substitution of the aliphatic chains. (author)

  17. Chemical treatment and biomimetic coating evaluating in zirconia-alumina ceramics; Avaliacao de tratamentos quimicos e recobrimento biomimetico em ceramicas de alumina-zirconia

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Amanda Abati

    2007-07-01

    Ceramic materials, as alumina and zirconia have been explored along the years as biomaterials application. The bio inert nature has been stimulating the development of new alternatives, as chemical treatments to improve the biological application of these ceramics. The biomimetic process of bio inert ceramics for coating apatite is based on soaking the implant in a simulated body fluid, SBF, with ion concentrations nearly equal to those of human blood plasma. The bioactivity of the material is related with the formation of a layer constituted of hydroxyapatite low crystalline, similar to the biological apatite. The biocompatibility associated to the structural properties of the alumina and zirconia has been stimulating the clinical use of these materials, mainly in areas of larger mechanical requests, places not recommended for bioactive hydroxyapatite, for instance. In this work samples of alumina, zirconia doped with Yttria (3% mol) and composites of alumina and zirconia doped with Yttria (3% mol) were prepared by co-precipitation method, calcinate, sintered, chemically treated with solutions of acid phosphoric and sodium hydroxide and them immersed in 1.0 M and 1.5 M SBF. The calcinate powders were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), gas adsorption (BET) and laser diffraction. The XRD results indicate that the samples are low crystalline. It was observed for BET that the samples present high specific surface area. The results of laser diffraction and SEM showed that the powders are agglomerates. The sintered samples were analyzed by XRD, SEM and X-ray fluorescence (XRF). The phases quantified by Rietveld method were: cubic, tetragonal and monoclinic of the zirconia, besides the phase alpha of the alumina. The chemical treatment with phosphoric acid didn't present a tendency of larger apatite formation in relation to the samples no chemically treated. The treatment with sodium hydroxide provoked accentuated transformation

  18. A geração química de vapor em espectrometria atômica Chemical vapor generation in atomic spectrometry

    Directory of Open Access Journals (Sweden)

    Iracema Takase

    2002-12-01

    Full Text Available The historical development of atomic spectrometry techniques based on chemical vapor generation by both batch and flow injection sampling formats is presented. Detection via atomic absorption spectrometry (AAS, microwave induced plasma optical emission spectrometry (MIP-OES, inductively coupled plasma optical emission spectrometry (ICP-OES , inductively coupled plasma mass spectrometry (ICP-MS and furnace atomic nonthermal excitation spectrometry (FANES are considered. Hydride generation is separately considered in contrast to other methods of generation of volatile derivatives. Hg ¾ CVAAS (cold vapor atomic absorption spectrometry is not considered here. The current state-of-the-art, including extension, advantages and limitations of this approach is discussed.

  19. Ordens não inteiras em cinética química Non-integer orders in chemical kinetics

    Directory of Open Access Journals (Sweden)

    André P. Oliveira

    2010-01-01

    Full Text Available Starting from zero-, first-, and second-order integrated laws for chemical kinetics, some cases are shown which produce fractional orders. Taking the Michaelis-Menten mechanism as a first example, it is shown that substrate order can go from 1 to zero, depending on relative concentration of enzyme and substrate. Using other examples which show fractional orders higher than one and even negative (inhibition, it is shown that the presence of an equilibrium before or parallel to the rate determining step can be the reason for fractional orders, which is an indication of a more complex mechanism.

  20. Mutagenic effect of accelerated heavy ions on bacterial cells

    Science.gov (United States)

    Boreyko, A. V.; Krasavin, E. A.

    2011-11-01

    features of energy transfer of the radiations that affect the character of induced DNA damage, and the efficiency inducible and constitutive cell repair systems. The growth of relative biological efficiency of heavy charged particles is determined by the growth of the damage yield of the DNA participating in the formation of radiation-induced effects, and higher efficiency of inducible repair systems. It was established that the LET value ( L max) for which the maximum (according to the applied irradiation criteria) coefficients of relative biological efficiency are observed varies depending on the character of the registered radiation induced effect. It was demonstrated that for gene mutations and induction of precision excision of mobile elements the values of L max are realized in a LET range of ≈20 keV/μm. For lethal effects of irradiation and induction of deletion mutations the value of L max is ≈ 100 and 50 keV/μm, respectively. The differences in the L max for the studied radiation gene effectis are determined by the different type of DNA damage participating in the mutation process. A molecular model of the formation of gene mutations in Escherichia coli cells under the action of ionizing radiation was proposed. Basic DNA radiation damage and main repair ways were considered in the framework of this model. The basis is the idea of the decisive role of mutagenic, error-prone, branch of SOS repair in fixing premutation DNA damage into point mutations. It was demonstrated that the central mechanism in this process is the formation of an inducible multi-enzymatic complex including the DNA polymerase V (Umu C), RecA-protease, SSB proteins, subunits of DNA polymerase III, performing erroneous DNA synthesis on the damaged matrix. A mathematical model of induction of gene mutations under ultraviolet cell irradiation was developed based on the molecular model.

  1. Mutagenic studies on the effect of Aldicarb "Temik" and vitamin C as antioxidant agent on the white rat:(Chromosomal aberrations and Micronucleus tests

    Directory of Open Access Journals (Sweden)

    Fatma M. Hamam* and Ihab H. Foda

    2004-12-01

    Full Text Available Widespread contamination of the environment due to increased and frequently indiscriminate usage of insecticides during the last two decades has aroused much concern over the possibility of their radiominetic effect. Evidence accumulating over the years emphasized the indisputable link between certain insecticides, chromosomal damage and possibility of gene mutation. There is a wide variety of insecticides, among which the carbamates. Their chemical relationship to ethyl carbamate makes them worthy of study for their possible deleterious effect on biological system. The main object of the present study is to evaluate the mutagenic effect of a carbamate insecticide" Aldicarb" alone and in combination of vitamin C as an antioxidant agent to decrease their mutagenicity. Male albino rats were tested orally for 48 hours , two doses of aldicarb were used in absence and in the presence of viamin C (1/4 and 1/10 LD50. The obtained data showed highly significant increase in the micronucleus (PCEM and in chromosomal aberrations in rat bone marrow cells at the two doses of aldicarb compared to control group. (P< 0.0001. The frequency of chromosomal aberrations and micronucleus decreased in rats treated with aldicarb and vitamin C than in aldicarb treated group. From these results we concluded that cytogenetic effect of aldicarb might be decreased by the usage of vitamin as an antioxidant agent.

  2. Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

    DEFF Research Database (Denmark)

    Jacobsen, Nicklas Raun; Møller, Peter; Cohn, Corey Alexander; Loft, Steffen; Vogel, Ulla; Wallin, Håkan

    The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg...

  3. Mutagenicity of algal metabolites of benzo(a)pyrene for Salmonella typhimurium

    International Nuclear Information System (INIS)

    The metabolism and growth effects of benzo(a)pyrene (BaP) were studied using a freshwater green alga, Selenastrum capricornutum. Algal cultures were incubated under gold light with BaP added at concentrations of 40, 160, 400, and 1200 μg/liter for the periods of 1-4 days. The metabolites and BaP were identified and quantified from ethyl acetate extracts of both algal cells and incubation medium. The ethyl acetate extracts were evaluated for genotoxicity using a micro-volume Salmonella typhimurium forward mutation assay with resistance to 8-azaguanine for selection. This assay detected the presence of small quantities of BaP and was particularly sensitive to the mutagenicity of BaP diols. Of those extracts prepared from algae and medium from cultures exposed to 400 μg BaP/liter (10 μg/25 ml culture), only algal cell extracts from one day's growth were mutagenic. In cultures exposed to 1200 μg BaP/liter (30 μg/25 ml culture), mutagenic materials were produced or persisted in both algae and media throughout the 4-day incubation. The observed mutagenic response can be attributed in part to the presence of unmetabolized BaP or to BaP diols

  4. Mutagenic activation of CL64,855, an anti-Trypanosoma cruzi nitroderivant, by bacterial nitroreductases

    Directory of Open Access Journals (Sweden)

    Morais Jr. Marcos Antonio de

    1998-01-01

    Full Text Available CL64,855 is a nitroimidazole-thiodiazole derivate with high anti-Trypanosoma cruzi activity. CL64,855-induced mutagenesis in the Salmonella/microsome test was detected by TA98 and TA98dnp6 strains, but not by the nitroreductase I-deficient TA98nr strain. The lack of mutagenic response of TA98nr was connected with its extreme resistance to the killing effect of the drug. Presence of S9 mix did not restore mutagenic activity of CL64,855 to the TA98nr strain. Additionally, CL64,855 was reduced in vitro by the nitroreductase I-proficient TA98 strain, mainly in the presence of oxygen, but not by the TA98nr strain. Mutagenic activity was detected in serum samples of treated guinea pigs by nitroreductase-proficient strains TA98 and TA98dnp6, but not by nitroductase-deficient strain TA98nr. In the case of urine, mutagenic activity was observed with all three tested strains, suggesting an in vivo metabolic activation of the drug by a distinct metabolic pathway.

  5. PATTERN RECOGNITION ANALYSIS OF A SET OF MUTAGENIC ALIPHATIC N-NITROSAMINES

    Science.gov (United States)

    A set of 21 mutagenic aliphatic N-nitrosamines were subjected to a pattern recognition analysis using ADAPT software. Four descriptors based on molecular connectivity, geometry and sigma charge on nitrogen were capable of achieving a 100% classification using the linear learning ...

  6. [In vivo mutagenicity and clastogenicity of ionizing radiation in nuclear medicine

    International Nuclear Information System (INIS)

    The overall goals of our research remains to investigate the mutagenic and clastogenic effects of exposure to low levels of ionizing radiation in human lymphocytes. We are studying hospital patients referred to a nuclear medicine department for diagnostic cardiac imaging and nuclear medicine technologists who administer radionuclides

  7. Short-term mutagenicity test systems for investigating the genetic toxicity of irradiated foods

    International Nuclear Information System (INIS)

    The current status of short-term tests for mutagenicity/carcinogenicity as alternatives to the long-term animal feeding tests in the study of genetic toxicology of irradiated foods is discussed in this review. Results of local studies on the toxicological safety of irradiated foods are also presented. (author)

  8. In vitro anti-inflammatory, mutagenic and antimutagenic activities of ethanolic extract of Clerodendrum paniculatum root

    Directory of Open Access Journals (Sweden)

    Pravaree Phuneerub

    2015-01-01

    Full Text Available Clerodendrum paniculatum L. (Family Verbenaceae has been used as an antipyretic and anti-inflammatory drug in traditional Thai medicine. This present study investigated the in vitro anti-inflammatory, mutagenic and antimutagenic activities of the ethanolic extract of C. paniculatum (CPE dried root collected from Sa Kaeo Province of Thailand. Murine macrophage J774A.1 cells were stimulated by lipopolysaccharide (LPS to evaluate nitric oxide (NO, tumor necrosis factor-α (TNF-α and prostaglandin E 2 (PGE 2 production in the anti-inflammatory test while the mutagenic and antimutagenic potential was performed by the Ames test. The outcome of this study displayed that the CPE root significantly inhibited LPS-induced NO, TNF-α, and PGE 2 production in macrophage cell line. In addition, the CPE root was not mutagenic toward Salmonella typhimurium strain TA98 and TA100 with and without nitrite treatment. Moreover, it inhibited the mutagenicity of nitrite treated 1-aminopyrene on both strains. The findings suggested the anti-inflammatory and antimutagenic potentials of CPE root.

  9. MUTAGENICITY OF TEFLON-COATED GLASS FIBER FILTERS: A POTENTIAL PROBLEM AND SOLUTIONS

    Science.gov (United States)

    Teflon-coated glass fiber filters, used in studies of airborne particulate matter, were tested for mutagenic activity using the Salmonella/mammalian-microsome (Ames) assay. For each sample, eight blank filters were simultaneously extracted with dichloromethane (DCM), and the extr...

  10. Molecular basis of the mutagenic and lethal effects of ultraviolet irradiation

    International Nuclear Information System (INIS)

    Using bacteria as a model, the molecular basis of the mutagenic and lethal effects of uv radiation is being studied. Attention is focused on the mechanism of action of uv-1 specific endonucleases in the repair of damaged DNA. The isolation and identification of similar enzymes in human cells are being conducted concurrently

  11. BIOASSAY-DIRECTED FRACTIONAL AND SALMONELLA MUTAGENICITY OF AUTOMOBILE AND FORKLIFT DIESEL EXHAUST PARTICLES

    Science.gov (United States)

    Abstract Many pulmonary toxicity studies of diesel exhaust particles (DEP) have used anautomobile-generated sample (A-DEP) whose mutagenicity has not been reported. In contrast,rnany inutagenicity studies of DEP have used a forklift-generated sample (SRM ...

  12. Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells

    Directory of Open Access Journals (Sweden)

    MÔNICA B.B. BELLE

    2013-06-01

    Full Text Available Contrast media (CM are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina® 292 (meglumine amidotrizoate and sodium-ionic dimmer in bone marrow cells (BMC of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina® 292 (3.0 mL/kg presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs in relation at the control group (P® 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.Meios de contraste (MC são freqüentemente utilizados em radiodiagnóstico e em radioterapia como uma ferramenta de diagnóstico e em planejamento do tratamento. Estudos prévios demonstraram que estes compostos induzem aberrações cromossômicas. Este estudo avaliou os efeitos mutagênicos induzidos pelo meio de contraste Urografina® 292 (Amidotrizoate de meglumina e sódio-dímero iônico em células da medula óssea (BMC de camundongos in vivo. Teste de micronúcleos foi realizado em BMC de camundongos CF-1, injetados com MC intravenoso nas doses 1,5 e 3,0 mL/kg e intraperitonealmente nas doses 1,0, 2,0 e 3,0 mL/kg. Os animais foram decapitados 24 horas após o tratamento por deslocamento cervical, e as BMC dos fêmures de cada animal foram utilizados no teste de micronúcleos. O grupo tratado com injeção intravenosa mais elevada de Urografina® 292 (3,0 mL/kg apresentou um aumento na freqüência de eritrócitos policromáticos micronucleados (MNPCEs em

  13. Fungos antagonistas e efeito de produtos químicos no controle da podridão parda em pomar de pessegueiro Antagonistic fungi and effect of chemical products in the control of brown rot in peach orchards

    Directory of Open Access Journals (Sweden)

    Luciene Martins Moreira

    2008-09-01

    Full Text Available Para verificar a eficiência de controladores biológicos e produtos químicos para controle da podridão parda, foi conduzido um experimento a campo na safra de 2002 no município da Lapa-PR, com a cultivar BR-1. O objetivo do experimento foi fazer seleção de tratamentos com controle biológico comparando-os com químicos em sistemas de manejo utilizados na região. Como tratamentos foram utilizados quatro antagonistas previamente selecionados em trabalho de pós-colheita, isolados F1, F2, F4 (Trichothecium roseum, isolado F9 (Penicillium sp (em 16 aplicações, sistema de Produção Integrada de Pêssegos (PIP (nove aplicações, fosfitos de Ca e de K + captan (11 aplicações, alternância de fungicidas (oito aplicações, tratamento convencional da propriedade (PC (nove aplicações, pulverizados desde a floração até a colheita, sendo a testemunha sem pulverização. Para avaliação, foi contado o número de frutos por ramo marcado após o raleio e no início da colheita. Para os frutos colhidos nos ramos foi determinada a incidência da doença na colheita e aos 3 e 5 dias em pós-colheita. O PIP, fosfito+captan, e isolado F4, não diferirem estatisticamente do padrão PC, e reduziram 95,5; 63,6 e 68,2%, respectivamente a doença em relação à testemunha que apresentou 44% dos frutos com podridão parda aos cinco dias. O tratamento com a alternância de fungicidas foi o melhor, e não foram observados frutos com a doença, entretanto apresentou uma redução do número de frutos entre o raleio e a colheita de 76,5% contra 50% em média dos demais tratamentos indicando um provável efeito fitotóxico.The efficiency of biological controllers and chemical products in the control of brown rot was investigated in a field experiment during the 2002 growing season in the municipality of Lapa-PR. The cultivar BR1 was used in the experiment. The objective of the experiment was to select treatments with biological control by comparing them

  14. SYBR Gold and SYBR Green II are not mutagenic in the Ames test.

    Science.gov (United States)

    Kirsanov, Kirill I; Lesovaya, Ekaterina A; Yakubovskaya, Marianna G; Belitsky, Gennady A

    2010-06-17

    Favorable photo-physical properties and high affinity to nucleic acids make new fluorescent cyanine dyes of the SYBR-type particularly useful for DNA and RNA visualization. The growing popularity of SYBR-type dyes is also explained by the fact that removal of the dye from the nucleic acids by ethanol precipitation is more efficient and less time-consuming than the phenol-chloroform extraction applied for the widely used phenanthridine DNA stain, ethidium bromide. To evaluate the safety of nucleic acid staining by SYBR Gold and SYBR Green II we compared the mutagenicity of these compounds, with characteristics corresponding to those of ethidium bromide, by use of the Salmonella/mammalian microsome reverse-mutation assays (Ames test). SYBR Green II and SYBR Gold did not show mutagenicity either in frame-shift or in base-substitution indicator strains, TA98 and TA100, respectively. These results were observed both in the presence and in the absence of supernatant from a rat-liver homogenate S9. Mutagenicity of these stains was not observed although their toxic concentration was reached. Toxic effects of SYBR Green II and SYBR Gold were seen approximately at the same molar concentrations as reported previously for SYBR Green I. As expected, ethidium bromide revealed strong mutagenicity with a maximum increase of 60-fold above the vehicle controls in the frame-shift indicator strain TA98 in the presence of rat-liver S9 extract. Thus, SYBR Gold and SYBR Green II do not show mutagenicity in our tests, even at toxic doses, and these DNA stains represent safer alternatives to ethidium bromide for nucleic acid visualization. PMID:20403457

  15. Atypical Role for PhoU in Mutagenic Break Repair under Stress in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Janet L Gibson

    Full Text Available Mechanisms of mutagenesis activated by stress responses drive pathogen/host adaptation, antibiotic and anti-fungal-drug resistance, and perhaps much of evolution generally. In Escherichia coli, repair of double-strand breaks (DSBs by homologous recombination is high fidelity in unstressed cells, but switches to a mutagenic mode using error-prone DNA polymerases when the both the SOS and general (σS stress responses are activated. Additionally, the σE response promotes spontaneous DNA breakage that leads to mutagenic break repair (MBR. We identified the regulatory protein PhoU in a genetic screen for functions required for MBR. PhoU negatively regulates the phosphate-transport and utilization (Pho regulon when phosphate is in excess, including the PstB and PstC subunits of the phosphate-specific ABC transporter PstSCAB. Here, we characterize the PhoU mutation-promoting role. First, some mutations that affect phosphate transport and Pho transcriptional regulation decrease mutagenesis. Second, the mutagenesis and regulon-expression phenotypes do not correspond, revealing an apparent new function(s for PhoU. Third, the PhoU mutagenic role is not via activation of the σS, SOS or σE responses, because mutations (or DSBs that restore mutagenesis to cells defective in these stress responses do not restore mutagenesis to phoU cells. Fourth, the mutagenesis defect in phoU-mutant cells is partially restored by deletion of arcA, a gene normally repressed by PhoU, implying that a gene(s repressed by ArcA promotes mutagenic break repair. The data show a new role for PhoU in regulation, and a new regulatory branch of the stress-response signaling web that activates mutagenic break repair in E. coli.

  16. Randomized controlled trial of dietary intervention: association between level of urinary phenolics and anti-mutagenicity.

    Science.gov (United States)

    Malaveille, Christian; Fiorini, Laura; Bianchini, Monica; Davico, Laura; Bertinetti, Sabrina; Allegro, Giovanni; Hautefeuille, Agnès; Sacerdote, Carlotta; Vineis, Paolo

    2004-07-11

    We have undertaken a randomized trial to confirm the ability of a class of phenolics, flavonoids, to increase urinary anti-mutagenicity in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, who were given three different diets. One diet was rich in flavonoids, but not based on supplementation ('flavonoid'), one was a normal iso-caloric diet with an adequate administration of fruit and vegetables ('normal'), and one was based on supplementation of flavonoids in the form of green tea and soy products ('supplement'). The urinary anti-mutagenicity-as inhibiting effect of the urinary extracts on the mutations induced by MeIQx-was measured in Salmonella typhimurium YG1024 in the presence of liver S9 from male Sprague-Dawley rats treated with Aroclor 1254. The amount of total phenolics in the urinary extracts was measured by use of spectrometric analysis. We found that important dietary modifications can be achieved through special recipes and instructions given by a cook during an intensive course. The intervention was focused on increasing the flavonoid intake, and it was successful in that respect. In fact, differences in flavonoid intake were appreciated mainly between the first group (normal diet) and the other two (flavonoid-rich and supplemented diet), suggesting that dietary modification can be as effective as supplementation. However, both urinary anti-mutagenicity and the amounts of urinary phenolics did not change as a consequence of the trial. These results suggest that only a small fraction of urinary phenolics is influenced by dietary changes in the intake of flavonoids, and that most urinary anti-mutagens and phenolics are metabolites of dietary flavonoids, whose formation is more affected by the activity and diversity of bacterial flora in the colon than by the quantity and type of intake. A strong correlation was found between urinary phenolics and anti-mutagenicity in all the groups involved in the trial. Such correlation

  17. Qualidade química de frutos do maracujazeiro-amarelo em solo com biofertilizante irrigado com águas salinas Chemical quality of yellow passion fruit in soil with biofertilizer and irrigated with saline water

    Directory of Open Access Journals (Sweden)

    Thiago J. Dias

    2011-03-01

    Full Text Available Um experimento foi desenvolvido para avaliar os efeitos da salinidade da água de irrigação e frequências de aplicação de biofertilizante bovino no solo sobre as qualidades químicas de frutos de maracujazeiro-amarelo. Os tratamentos foram distribuídos em arranjo fatorial 5 x 4, correspondendo aos valores de condutividade elétrica da água de irrigação: 0,5; 1,5; 2,5; 3,5 e 4,5 dS m-1 e a quatro frequências de aplicação do biofertilizante: sem a aplicação do biofertilizante (SB; aplicação realizada uma semana antes do transplantio (1 SAT; a cada 90 dias a partir do transplantio (90 DAT; uma semana antes do transplantio e a cada 90 dias após o transplantio (1 SAT + 90 DAT. Pelos resultados se certifica de que o aumento da salinidade da água inibiu o rendimento em polpa, os teores de sólidos solúveis (SS, o pH, a razão sólidos solúveis/acidez titulável (SS/AT e os teores de vitamina "C" no suco, proporcionando maior acidez e elevação da condutividade elétrica do suco. O aumento da frequência de aplicação do biofertilizante, apesar de aumentar a condutividade elétrica do suco atenuou os efeitos degenerativos dos sais da água de irrigação ás plantas, resultando em incremento no rendimento em polpa, teores de vitamina "C" e ainda reduziu a razão SS/AT e o pH da polpa.This work had the objective to evaluate the effects of irrigation water salinity (EC associated to application frequencies of bovine biofertilizer on chemical qualities of yellow passion fruits. The treatments were distributed using 5 x 4 factorial design corresponding the five EC of irrigation plant water of 0.5, 1.5, 2.5, 3.5 and 4.5 dS m-1, in four frequencies of application of the biofertilizer: without bovine biofertilizer (SB; with application one week before transplanting (1 SAT; application with intervals of 90 days after transplanting (90 DAT; and, with application one week before transplanting and at intervals of 90 days (1 SAT + 90 DAT. From

  18. Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems Genotoxicidade e mutagenicidade diretas de substâncias e materiais endodônticos avaliadas por dois testes procariotas

    Directory of Open Access Journals (Sweden)

    Sérgio Márcio Motta Gahyva

    2005-12-01

    Full Text Available Substances and materials used in endodontic therapy come into close contact with the periradicular tissues via apical foramen and foramina. Consequently, they should possess biocompatibility. There are currently few studies describing the genotoxic and mutagenic potentials of substances and materials used in endodontics. The purpose of this study was to evaluate the direct genotoxic and mutagenic properties of several substances and materials used in different phases of the endodontic treatment. For this intent, two prokaryotic test systems were used: the SOS chromotest and the Ames test. No metabolization with S9 was investigated, since only the direct effects of the substances and materials were surveyed. Most of the substances and materials tested presented mild to moderate cytotoxicity and genotoxicity as revealed by the SOS chromotest. Formocresol was the only tested substance to present severe genotoxicity to the tester bacterial strains. However, no substance or material tested showed direct mutagenicity as revealed by the Ames test.Substâncias e materiais utilizados na terapia endodôntica entram em íntimo contato com os tecidos perirradiculares via forame apical e foraminas e, em decorrência disto, deveriam idealmente possuir as seguintes características: biocompatibilidade e ausência de mutagenicidade. Existem poucos estudos avaliando o potencial genotóxico e mutagênico de substâncias e materiais utilizados em Endodontia. Este estudo avaliou os efeitos citotóxicos, genotóxicos e mutagênicos diretos de várias substâncias e materiais utilizados em diferentes etapas do tratamento endodôntico. Para isto, dois sistemas procarióticos foram usados: o SOS chromotest e o teste de Ames. Metabolização com fração S9 não foi realizada, uma vez que tencionou-se avaliar os efeitos diretos das substâncias e materiais. Os resultados demonstraram que algumas substâncias e materiais foram citotóxicos e/ou genotóxicos no SOS

  19. <em>Botulinum Neurotoxinem> A Injections Influence Stretching of the Gastrocnemius Muscle-Tendon Unit in an Animal Model

    Directory of Open Access Journals (Sweden)

    Christopher J. Tuohy

    2012-08-01

    Full Text Available <em>Botulinum Neurotoxinem> A (BoNT-A injections have been used for the treatment of muscle contractures and spasticity. This study assessed the influence of (BoNT-A injections on passive biomechanical properties of the muscle-tendon unit. Mouse gastrocnemius muscle (GC was injected with BoNT-A (<em>n> = 18 or normal saline (<em>n> = 18 and passive, non-destructive, <em>in vivoem> load relaxation experimentation was performed to examine how the muscle-tendon unit behaves after chemical denervation with BoNT-A. Injection of BoNT-A impaired passive muscle recovery (15% <em>vs.> 35% recovery to pre-stretching baseline, <em>p> < 0.05 and decreased GC stiffness (0.531 ± 0.061 N/mm <em>vs.> 0.780 ± 0.037 N/mm, <em>p> < 0.05 compared to saline controls. The successful use of BoNT-A injections as an adjunct to physical therapy may be in part attributed to the disruption of the stretch reflex; thereby modulating <em>in vivoem> passive muscle properties. However, it is also possible that BoNT-A injection may alter the structure of skeletal muscle; thus modulating the <em>in vivoem> passive biomechanical properties of the muscle-tendon unit.

  20. Chemically induced mutagenesis in Blackgram(Vigna mungo (L.Hepper

    Directory of Open Access Journals (Sweden)

    Y.Anbu Selvam,R.Elangaimannan,M.Venkatesan,P.Karthikeyan and K.Palaniraja

    2010-07-01

    Full Text Available The present investigation was carried out to study the extent of variability existed in the rice fallow blackgram varietiesnamely VBN3 and ADT 3 after induction of mutation through chemical mutagens namely EMS and colchicine. The resultsof M1 generation revealed that there was a shift in the mean for the characters Viz., plant height, number of branches perplant, number of clusters per plant, number of pods per plant,100 seed weight and seed yield per plant towards positive/negative directions.In M2, chlorophyll mutants namely, albina, xantha, chlorine, viridis and few viable mutants like giantplant, bushy plant, unifoliate leaf, narrow leaf, crinkled leaf, glabrous pod, short pod ,brown seeded, sterile plant andpigmented mutants were recorded.A significant increase in number of branches per plant, number of clusters per plant,number of pods per plant and seed yield per plant was noticed in M2 generation.The PCV and GCV showed significantlyhigher values in yield component characters such as number o f branches per plant, number of pods per cluster, number ofpods per plant and seed yield per plant in M2 generation.The yield attributing traits like number of branches per plant,number of pods per cluster,100 seed weight and seed yield per plant recorded high heritability coupled with high geneticadvance as per cent of mean in M2 generation.

  1. Mutagenicity of γ-irradiated oxygenated and deoxygenated solutions of 2-deoxy-D-ribose and D-ribose in Salmonella typhimurium

    International Nuclear Information System (INIS)

    Solutions of 2-deoxy-D-ribose and D-ribose were γ-irradiated under different experimental conditions and tested for mutagenicity, with and without preincubation, in Salmonella typhimurium. The irradiated sugar solutions were mutagenic in the tester strains TA 100 and TA 98. Except for malonaldehyde (MDA), which is not mutagenic in the concentrations produced radiolytically, the relative mutagenicities of the individual radiolytic products are unknown. With irradiated solutions of 2-deoxy-D-ribose, a relationship was found between the level of non-MDA aldehydes and the mutagenicity in TA 100. Heating the irradiated solutions of 2-deoxy-D-ribose resulted in a temperature-dependent reduction fo the mutagenicity. Autoclaved, non-irradiated solutions of 2-deoxy-D-ribose were not mutagenic in the Salmonella test. (orig.)

  2. Obtenção e caracterização de materiais argilosos quimicamente ativados para utilização em catálise Preparation and characterization of chemically activated clays for use in catalysis

    Directory of Open Access Journals (Sweden)

    M. G. F. Rodrigues

    2006-12-01

    Full Text Available O principal objetivo deste trabalho é caracterizar argilas esmectíticas "Chocolate" de Boa-Vista, PB, tanto na sua forma natural quanto tratada quimicamente, visando sua aplicação em catálise. As argilas foram caracterizadas por fluorescência de raios X, difração de raios X e espectrofotometria de absorção óptica na região do infravermelho. Os resultados mostraram que o tratamento ácido alterou a estrutura da montmorilonita. A reação de isomerização do meta-xileno, para o material ativado, mostrou que a argila ácida foi mais ativa que a natural.The main objective of this paper is to characterize the chocolate smectite clays of Boa-Vista, PB, Brazil, in their natural form, and after chemical treatment, for their application in catalysis. The experimental methods were: X-ray fluorescence, X-ray diffraction and infrared spectroscopy. The results show that the acid treatment altered the montimorillonite structure. The obtained reaction (isomerization of m-xylene for the treated material showed that treated clays were more active than the natural ones.

  3. Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

    Directory of Open Access Journals (Sweden)

    Claudia R. de Rainho

    2010-12-01

    Full Text Available N-nitroso compounds, such as N-nitrosodiethylamine (NDEA, can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water and positive control maleic hydrazide (MH 30mg/mL. After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM, showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dose-dependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.Nitrocompostos, como N-nitrosodietilamina (NDEA, podem ser formados pela reação entre uma amina secundária e agentes nitrosantes e são suspeitos de estarem envolvidos na formação de tumores em humanos. NDEA é considerada um carcinógeno fraco e ensaios genotóxicos provavelente pela utilização de um sistema de ativação ineficiente e/ou pela utilização de um eficiente sistema de reparo. Neste trabalho, nós avaliamos a sensibilidade de ensaios com Alliu cepa L. e Tradescantia pallida var. purpurea (Trad-SH à genotoxicidade e mutagenicidade induzidas por diferentes concentrações de NDEA (0,1; 0,5; 5 e 25mM por 3h, por 3 dias consecutivos, incluindo controle

  4. Onderzoek naar de mutagene werking van acrylonitril met microorganismen Drosophila melanogaster en L5178Y muize-lymfoomcellen

    OpenAIRE

    Voogd CE; van der Stel JJ; Verharen HW; Kramers PGN; Mout HCA; Knaap AGAC; Langbroek PG

    1985-01-01

    Acrylonitril was mutageen bij 5 mmol/l in de fluctuatietest zonder metabolische activering ; in gasfase werd bij 0,5 mmol/l lucht een mutagene werking gevonden. Deze stof was ook mutageen in de Ames-test voor Salmonella typhimurium TA1535 (15 mmol/plaat) met metabolische activering doch niet met de stammen TA98 en TA100. In de gasfase werd in de Ames-test geen mutagene werking gevonden, groeiremming trad op bij ca. 0,1 mmol/l lucht. Met acrylonitril werd geen mutagene werking gevonden op Dros...

  5. Atributos químicos do solo decorrentes da aplicação em superfície de calcário e gesso em sistema plantio direto recém-implantado Chemical soil attributes as affected by lime and phosphogypsum surface application in a recently established no-tillage system

    Directory of Open Access Journals (Sweden)

    Rogério Peres Soratto

    2008-04-01

    profundidade de 0-0,20 m com a calagem foram menores do que os estimados pelo método da elevação da saturação por bases, principalmente nas maiores doses, mesmo com a aplicação de gesso agrícola. A calagem em superfície não alterou os teores de micronutrientes na camada de 0-0,20 m de profundidade.There is interest to establish the no-tillage system in areas cultivated previously with conventional tillage or pasture, with surface liming after system establishment. This practice is possible provided that there are no physical obstacles for root growth. Phosphogypsum is an alternative to decrease Al3+ and increase base saturation (BS, mainly Ca2+, in the subsoil, and can be used complementary to liming. The objective of this study was to evaluate the changes in chemical soil attributes (pH CaCl2, H + Al, Al3+, Ca2+, Mg2+, S-SO4(2-, BS, and content of cationic micronutrients as affected by lime and phosphogypsum surface application in a recently established no-tillage system in dry-winter region. The experiment was carried out on a dystroferric Haplorthox, in Botucatu County, São Paulo State, Brazil. A randomized complete block design, in split-plot scheme and four replications, was used. The plots consisted of four dolomitic limestone rates (0; 1,100; 2,700; and 4,300 kg ha-1, intended to reach a BS to 50, 70 and 90 %, respectively. The subplots were represented by the absence or presence of phosphogypsum application (2,100 kg ha-1. Soil samples were collected 3, 6, 12, and 18 months after lime and phosphogypsum application, at depths of 0-0.05, 0.05-0.10, 0.10-0.20, 0.20-0.40, 0.40-0.60, and 0-0.20 m. Lime surface application reduced soil and increased exchangeable Ca and Mg contents, mainly in the top soil layers. Phosphogypsum application promoted increasing exchangeable Ca and S-SO4(2- contents, and decreased exchangeable Al in the soil, favoring the effects of surface liming that influenced the subsoil layers more rapidly. Base saturation values obtained at a

  6. Characterization of chemical compounds for dosimetry of the radiation in industrial processes; Caracterizacao de compostos quimicos para dosimetria das radiacaoes em processos industriais

    Energy Technology Data Exchange (ETDEWEB)

    Galante, Ana Maria Sisti

    1999-07-01

    Different chemical compounds have been studied to optimize dosimetric systems in irradiation processes. In this study 2,3,5 Triphenyl -2H- Tetrazolium Chloride, Brilliant Cresyl Blue, Bromocresol Green and Potassium Nitrate were investigated for their merits or faults, for {sup 60} Co gamma field, in order to verify if can be considered as dosimeters. Fricke solution was used as reference dosimeter to determine absorption dose rates at the gamma facilities.Only Bromocresol Green and Potassium Nitrate are recommended for dosimetry purposes since the main characteristics were achieved. The other two compounds could be used in dosimetry with changes in their formulation. Bromocresol Green and potassium Nitrate are reproducible and radiation sensitive for absorbed doses from 300 Gy to 150 kGy Bromocresol Green was used in liquid form and Potassium Nitrate was prepared in solid pellets form. Spectrophotometry in the visible region was used as the main detection technique, which allows relating optical absorption, before and after irradiation, with the absorbed dose. The maximum absorption wavelength for each compound was observed at 450-460nm for bromocresol Green and 546nm for Potassium Nitrate. Dose calibration curves are linear for both compounds in all dose intervals. When irradiated with accelerated electrons, with energies between o,9 MeV and 1,5MeV, optical absorption intensification, of about 2,6 times, was observed when comparing results for Potassium Nitrate, with those for gamma rays. All the evaluations are presented in this work. (author)

  7. Método titrimétrico para determinar fosfito em amostras agroindustriais Titrimetric method for phosphite determination in agricultural chemical samples

    Directory of Open Access Journals (Sweden)

    Vanessa Pezza Franzini

    2007-04-01

    Full Text Available A new titrimetric method for the determination of phosphite in fertilizer samples, based on reaction of H3PO3 with standard iodine solution in neutral media, is proposed. Diluted samples containing ca. 0.4% m/v P2O5 are heated and titrated with 0.05 mol L-1 iodine standard until the solution becomes faint yellow. Back titration is also feasible: a slight excess of titrant is added followed by starch indicator and titration is completed taking as the end point the change in color from blue to colorless. The influence of chemical composition and pH of buffers, temperature and foreign species on waiting time and end-point detection were investigated. For the Na2HPO4/NaH2PO4 buffer (pH 6.8 at 70 °C, the titration time was 10 min, corresponding to about 127 mg iodine, 200 mg KI and 174 mg Na2HPO4 and 176 mg NaH2PO4 consumed per determination. Accuracy was checked for phosphite determination in seven fertilizer samples. Results obtained by the proposed procedure were in agreement with those obtained by spectrophotometry at 95% confidence level. The R.S.D. (n=10 for direct and back titration was 0.4% and 1.3% respectively.

  8. Composição química e degradabilidade in situ da ração em ovinos em área de caatinga no sertão de Pernambuco Chemical composition and in situ digestibility of diets for sheep in the caatinga region

    Directory of Open Access Journals (Sweden)

    Gladston Rafael de Arruda Santos

    2009-02-01

    Full Text Available Objetivou-se avaliar o efeito do tipo de fístula e da hora de coleta sobre a composição química e a degradabilidade in situ da ração em ovinos criados na caatinga. Foram utilizados dez ovinos mestiços de Santa Inês (cinco com cânula no rúmen e cinco com cânula no esôfago recebendo água e mistura mineral ad libitum. Os percentuais de matéria seca (MS variaram de 11,99 a 25,28%, matéria mineral (MM, 10,92 a 14,44%, proteína bruta (PB, 10,64 a 17,19%, extrato etéreo (EE, 2,95 a 4,77%, fibra em detergente neutro (FDN, 54,83 a 63,14%, fibra em detergente ácido (FDA, 39,40 a 46,62%, proteína insolúvel em detergente neutro (PIDN, 49,74 a 57,95%, proteína insolúvel em detergente ácido (PIDA, 28,52 a 39,15%, carboidratos totais (CT, 65,40 a 72,73%, carboidratos não-fibrosos (CNF, 5,47 a 12,86%, fenóis totais (FT, 0,37 a 0,52%, taninos totais (TT, 0,16 a 0,28% e taninos condensados (TC, 1,28 a 6,24%, respectivamente. A degradabilidade potencial variou de 48,25 a 64,63% para MS; 36,43 a 54,34% para FDN; e de 62,13 a 77,24% para proteína; a fração B, de 35,77 a 47,78% para MS; 33,28 a 50,38% para FDN; e 35,44 a 56,09% para proteína; a fração C, de 4,60 a 13,40% para MS; 3,84 a 8,42% para FDN; e 5,37 a 14,36% para proteína; a fração A, 9,74 a 18,13% para MS; 2,16 a 4,41% para FDN; e 20,21 a 31,49% para proteína; a degradabilidade efetiva para a taxa de passagem de 2%/hora, de 43,28 a 55,71 para MS; 29,21 a 36,54 para FDN; 55,84 a 67,49 para proteína; e a degradabilidade efetiva para a taxa de passagem de 5%/hora, de 37,60 a 47,27 para MS; 23,02 a 33,33 para FDN; e 45,74 a 59,99 para proteína. A composição química e a degradabilidade in situ da ração em ovinos foi influenciada pelo mês de avaliação. A dieta apresentou baixa digestibilidade in situ da MS, FDN e PB. A fistula ruminal, em comparação à fistula de esôfago, permitiu melhor avaliação da dieta, em virtude da recuperação total da extrusa.This work

  9. Efeitos do processamento químico e da esterilização em Óxido de etileno em osso cortical e esponjoso de ratas: estudo com microscopia de luz e eletrônica de varredura Effects of chemical processing and oxide ethylene sterilization on cortical and cancellous rat bone: a light and electron scanning microscopy study

    OpenAIRE

    Marcello Teixeira Castiglia; Juliano Voltarelli F. da Silva; José Antonio Thomazini; José Batista Volpon

    2009-01-01

    OBJETIVO: Avaliar, sob o ponto de vista microscópico, modificações estruturais do osso esponjoso e cortical, após serem submetidos a processamento químico e esterilização em óxido de etileno. MÉTODOS: Amostras de osso esponjoso e cortical foram obtidas de fêmures de ratas albinas jovens (Wistar)e separadas em quatro grupos contendo osso cortical e esponjoso: I- Fragmentos secos em estufa; II- Fragmentos secos em estufa e esterilizados em óxido de etileno; III- Fragmentos processados quimicame...

  10. Ultrasound-Assisted Extraction of Carnosic Acid and Rosmarinic Acid Using Ionic Liquid Solution from <em>Rosmarinus officinalisem>>

    Directory of Open Access Journals (Sweden)

    Chunjian Zhao

    2012-09-01

    Full Text Available Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from <em>Rosmarinus officinalisem>. Eight ionic liquids, with different cations and anions, were investigated in this work and [C8mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid–liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from <em>R. officinalisem>> em>compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

  11. Stability of mutagenic tautomers of uracil and its halogen derivatives: the results of quantum-mechanical investigation

    Directory of Open Access Journals (Sweden)

    Hovorun D.M.

    2010-07-01

    Full Text Available Aim. To investigate using the quantum-mechanical methods uracil (Ura intramolecular tautomerisation and the effect of the thymine (Thy methyl (Me group substitution by the halogen on that process. Methods. Non-empirical quantum mechanic, analysis of the electron density by means of Bader’s atom in molecules (AIM theory and physicochemical kinetics were used. Results. For the first time it has been established that the substitution of thymine Me-group for the halogen (Br, F, Cl has practically no effect on the main physico-chemical characteristics of intramolecular tautomerisation. At the same time, the energy of Ura tautomerisation increases for 3,08 kcal/mol in comparison with corresponding value for Thy under standard conditions. Conclusions. So, Thy, unlike Ura, is obviously able, as a canonical DNA nucleotide base, to provide together with Ade, Gua and Cyt an acceptable mutability degree of the genome from the point of view of its adaptation reserve. Mutagenic action of the Ura halogen derivatives is not directly associated with their tautomerisation.

  12. Inter-annual chemical stratification in Brazilian natural lakes: meromixis and hypolimnetic memory Estratificação química em lagos naturais brasileiros: meromixia e memória hipolimnética

    Directory of Open Access Journals (Sweden)

    Luciana Gomes Barbosa

    2012-06-01

    Full Text Available AIM: chemical stratification and the patterns of light limitation and nutrients of two natural lakes, one shallow and the other one deep, were comparatively evaluated, both lakes located in the southeast Brazil. METHODS: pH, electrical conductivity, dissolved oxygen, total dissolved solids and nutrients were monthly collected during 5 consecutive years at the vertical profile of the two lakes. RESULTS: multivariate analysis indicated that the long thermal stratification period favored the occurrence of chemical stratification in the two lakes. However, in the deeper lake the stratified thermal profile with high hypolimnetic nutrient concentration, electrical conductivity, total dissolved solids and redox potential indicated that the mixing was not complete even during the annual circulation period, suggesting a slight meromixis and a high chemical stability at the hypolimnion. In the shallower lake, high light attenuation and high availability of nitrogen forms (mainly N-NH4 and phosphorus was observed along the water column, even during stratification. In the deeper lake, N and P co-limitation and low light attenuation coefficients were detected. CONCLUSION: thermal and chemical stratification patterns indicated that the Carioca lake is a shallow, turbid, nutrient rich, whereas the Dom Helvecio lake is a deep, clear, oligotrophic system with a tendency towards meromixis and the isolation of solutes in the hypolimnion. Consequently, meromixis was compared to a "hypolimnetic memory", which was defined, in the case of the deeper lake, as the maintenance of the chemical stratification along the years, during the lake thermal circulation period.OBJETIVO: a estratificação química e os padrões de limitação por luz e nutrientes foram avaliados comparativamente em dois lagos naturais, sendo um raso e o outro profundo no sudeste do Brasil. MÉTODOS: as amostragens de pH, condutividade elétrica, oxigênio dissolvido, sólidos totais dissolvidos e

  13. Systematic approach for assessment of accident risks in chemical and nuclear processing; Abordagem sistematica para avaliacao de riscos de acidentes em instalacoes de processamento quimico e nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Senne Junior, Murillo

    2003-07-15

    The industrial accidents which occurred in the last years, particularly in the 80's, contributed a significant way to draw the attention of the government, industry and the society as a whole to the mechanisms for preventing events that could affect people's safety and the environment quality. Techniques and methods extensively used the nuclear, aeronautic and war industries so far were adapted to performing analysis and evaluation of the risks associated to other industrial activities, especially in the petroleum, chemistry and petrochemical areas. The risk analysis in industrial facilities is carried out through the evaluation of the probability or frequency of the accidents and their consequences. However, no systematized methodology that could supply the tools for identifying possible accidents likely to take place in an installation is available in the literature. Neither existing are methodologies for the identification of the models for evaluation of the accidents' consequences nor for the selection of the available techniques for qualitative or quantitative analysis of the possibility of occurrence of the accident being focused. The objective of this work is to develop and implement a methodology for identification of the risks of accidents in chemical and nuclear processing facilities as well as for the evaluation of their consequences on persons. For the development of the methodology, the main possible accidents that could occur in such installations were identified and the qualitative and quantitative techniques available for the identification of the risks and for the evaluation of the consequences of each identified accidents were selected. The use of the methodology was illustrated by applying it in two case examples adapted from the literature, involving accidents with inflammable, explosives, and radioactive materials. The computer code MRA - Methodology for Risk Assessment was developed using DELPHI, version 5.0, with the purpose of

  14. Preussianone, a New Flavanone-Chromone Biflavonoid from <em>Garcinia preussiiem> Engl.

    OpenAIRE

    Gerhard Bringmann; Kurt Hostettmann; Jean-Luc Wolfender; Alain Meli Lannang; Nkengfack, Augustin E; Barbara Hertlein-Amslinger; Karine Ndjoko-Ioset; Bernadette Biloa Messi

    2012-01-01

    A new flavanone-chromone biflavonoid, preussianone (1), has been isolated from the leaves of <em>Garcinia preussiiem>, along with four known biflavonoids. The absolute stereostructures were elucidated by chemical, spectroscopic, and chiroptical methods. The biological properties of the new biflavonoid against several bacterial strains were evaluated.

  15. Antibacterial Secondary Metabolites from the Cave Sponge <em>Xestospongia em>sp.

    Directory of Open Access Journals (Sweden)

    Sridevi Ankisetty

    2012-05-01

    Full Text Available Chemical investigation of the cave sponge <em>Xestospongia em>sp. resulted in the isolation of three new polyacetylenic long chain compounds along with two known metabolites. The structures of the new metabolites were established by NMR and MS analyses. The antibacterial activity of the new metabolites was also evaluated.

  16. Fracionamento a seco da farinha de aveia e modificação química da fração rica em amido Dry fractionation of oatmeal and chemical modification of starch rich fraction

    Directory of Open Access Journals (Sweden)

    Ana Paula Daniel

    2006-12-01

    Full Text Available O estudo teve por objetivo obter frações de farinha de aveia enriquecidas em amido e em fibras pelo fracionamento a seco e modificar quimicamente (fosfatação o amido da fração rica neste constituinte, avaliando suas propriedades funcionais. O fracionamento foi efetuado utilizando-se as granulometrias > 300, 212-300, 150-212, e 300 µm e 212-300 µm foram semelhantes, com aumento de aproximadamente 1,5 e 2,7 vezes nos teores de proteína e fibra, e redução de 0,5-0,6 vezes no teor de amido, quando comparadas à farinha integral. As frações 212-150 µm e The objective of this study was to obtain starch and fiber enriched oatmeal fractions through sieving, chemically modifying (phosphorylation the starch enriched fraction, and evaluating its functional properties. Fractionation was performed using > 300, 212-300, 150-212, and 300 µm and 212-300 µm were similar and had increased protein and fiber content (1.5 and 2.7 fold, but reduced starch content (0.5-0.6 fold when compared to whole oatmeal. Fractions 212-150 µm and < 150 µm were similar and had the highest starch content (around 70%. The starch-rich fraction < 150 µm that had the highest yield during sieving (35.5% was phosphorylated with sodium tripolyphosphate at 150-155 °C for 20 and 40 min, yielding 0.39 and 0.32% phosphorus bound, respectively. Cold water binding capacity increased (1.9-3.3 fold, while syneresis at 5 °C or after freezing/thawing was significantly reduced (6-20 and 5-6 fold, respectively in phosphorylated starch fraction when compared to the native starch fraction. Phosphorylation reduced the increase of pasta opacity during storage at 5 °C, which indicates a lower retrogradation tendency. Thus, oatmeal sieving yielded fractions either enriched in fiber and protein or enriched in starch. Moreover, phosphorylation of the starch-rich fraction improved their functional properties, which may increase the potential applicability and economic value of this

  17. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Directory of Open Access Journals (Sweden)

    Dao-Yuan Zhang

    2012-06-01

    Full Text Available <em>Eremosparton songoricum em>(Litv. Vass. (<em>E. songoricumem> is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various <em>E. songoricumem> plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions.<em> EsEFem> and <em>Esα-TUB> were sufficient for various stress conditions, <em>EsEF> and <em>EsACT> were suitable for samples of differing germination stages, and <em>EsGAPDH>and <em>Es>UBQ em>were most stable across multiple adult tissue samples. The <em>Es18Sem> gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor <em>EsDREB2em>> em>verified the utility of<em> E. songoricumem> reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in <em>E. songoricumem>, and these data will facilitate future work on gene expression in this species.

  18. Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, D. L.; Sass, E. J.; Fritz, L. K.; Sasser, L. B.

    1989-07-31

    The mutagenic potential of lewisite was evaluated in the standard plate incorporation method and by the preincubation modification of the Ames Salmonella/microsomal assay with tester strains TA97, TA98, TAlOO and TA102. All strains were tested with activation (20 and 50 {micro}l/ plate) and without activation. The lewisite was screened initially for toxicity with TA98 over a range of concentrations from 0.01 to 250 {micro}g of material per plate. However, concentrations selected for mutagenicity testing were adjusted to a range of 0.001 to 5 {micro}g/plate because of the sensitivity of tester strain TA102, which exhibited cytotoxicity at 0.01 ug/plate. No mutagenic response was exhibited by any of the strains in either method used. All other tester strains showed evidence of cytoxicity (reduction in mutagen response or sparse background lawn) at 5.0 {micro}g/plate or lower.

  19. Responses of the L5178Y mouse Lymphoma cell forward mutation assay. V: 27 coded chemicals.

    Science.gov (United States)

    McGregor, D B; Brown, A G; Howgate, S; McBride, D; Riach, C; Caspary, W J

    1991-01-01

    Twenty-seven chemicals were tested for their mutagenic potential in the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay using procedures based upon those described by McGregor et al. (McGregor DB, Martin R, Cattanach P, Edwards I, McBride D, Caspary WJ (1987): Environ Mol Mutagen 9:143-160). Cultures were exposed to the chemicals for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 micrograms/ml. The chemicals were tested at least twice. Statistically significant responses were obtained with acid orange 10, aniline, benzaldehyde, o-chloroaniline, chlorodibromomethane, cytembena, 1,2-dibromo-4-(1,2-dibromomethyl) cyclohexane, dieldrin, lithocholic acid, oxytetracycline, phenazopyridine HCl, 1-phenyl-3-methyl-5-pyrazolone, sodium diethyldithiocarbamate, solvent yellow 14, tetraethylthiuram disulfide (disulfiram), 2,4-toluene diisocyanate, and 2,6-toluene diisocyanate. Apart from phenazopyridine HCl, acid orange 10, and solvent yellow 14, rat liver S9 mix was not a requirement for the mutagenic activity of these compounds. Chemical not identified as mutagens were N-4-acetylaminofluorene, chlorpheniramine maleate, chloropropamide, 1,4-dioxane, endrin, ethylene glycol, iron dextran, methapyrilene, sodium(2-ethylhexyl)alcohol PMID:1902415

  20. Guidelines of Italian CCTN for classification of some effects of chemical substances

    Energy Technology Data Exchange (ETDEWEB)

    Mucci, N. [ISPESL, Monteporzio Catone, Rome (Italy). Dip. di Medicina del Lavoro; Camoni, I. [Ist. Superiore di Sanita`, Rome (Italy). Lab. di Tossicologia Applicata

    1996-03-01

    Definitions of the categories and the criteria for the classification of chemical substances on the basis of their potential carcinogenic, mutagenic and toxic-reproductive effects, elaborated by the Italian National Advisory Toxicological Committee (CCTN) in 1994. Besides all the allocations effected by the CCTN in the period 1977-1995 are reported, updated according to these criteria.

  1. Micronuclei frequency in children exposed to environmental mutagens: a review

    DEFF Research Database (Denmark)

    Neri, Monica; Fucic, Aleksandra; Knudsen, Lisbeth E;

    2003-01-01

    selected from the HUMN database. An effect of chronic and infectious diseases on MN levels has been reported by various authors. Most studies describing the effect of exposure to genotoxic agents (ionizing radiation, chemicals, drugs, environmental tobacco smoke) found an increase of MN in exposed children....... The limited number of published papers indicates that the conduct of properly designed studies on the effect of environmental pollutants in children may be difficult. This review confirmed the usefulness of MN assay in biomonitoring studies conducted in children, revealing that in many circumstances...

  2. Use of orbitrap-MS/MS and QSAR analyses to estimate mutagenic transformation products of iopamidol generated during ozonation and chlorination.

    Science.gov (United States)

    Matsushita, Taku; Hashizuka, Masahiro; Kuriyama, Taisuke; Matsui, Yoshihiko; Shirasaki, Nobutaka

    2016-04-01

    The effects of two water purification processes (ozonation, and chlorination after ozonation) on the mutagenicity of a solution containing iopamidol (X-ray contrast medium) were investigated by using the Ames assay. No mutagenicity was observed during ozonation. In contrast, mutagenicity was induced by the ozone-treated iopamidol-containing solution after subsequent chlorination, indicating that mutagenic transformation-products (TPs) were generated. Ten of 70 peaks detected on the LC/MS total ion chromatogram (TIC) of the ozone-treated iopamidol-containing solution after chlorination had a positive correlation (r(2) > 0.6) between their peak areas and the observed mutagenicity, suggesting that TPs detected as these peaks may induce mutagenicity. To narrow down the possible contributors to the observed mutagenicity, we compared the areas of the peaks on the TIC-charts with and without chlorination. Of the ten peaks, six were also detected in the ozone-treated iopamidol-containing solution without chlorination, which did not induce mutagenicity, indicating that these peaks were not related to the observed mutagenicity. Accurate m/z values and MS/MS analysis with an orbitrap MS of the remaining four peaks revealed that two of them represented the same TP in the negative and positive ion modes. The three remaining TPs were assessed in four quantitative structure-activity relationship models for predicting Ames mutagenicity. At least one model predicted that two of the three TPs were mutagenic, whereas none of the models predicted that the other TP was a mutagen, suggesting that the former TPs, estimated as N1-acetyl-5-amino-6-chloro-2-iodobenzene-1,3-dicarboxamide and 3-hydroxy-2-{3-[(2-hydroxyethoxy)carbonyl]-2,4,6-triiodo-5-nitrobenzoyl}amino)propanoic acid, could be the candidate compounds that contributed to the observed mutagenicity. PMID:26807944

  3. Avaliação da composição química de milho seco e armazenado em silo tipo alambrado com ar natural forçado Evaluation of chemical composition of corn dried and stored with forced natural air

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Gutkoski

    2009-12-01

    Full Text Available O trabalho objetivou estudar o efeito da umidade de colheita e do tempo de armazenamento em grãos de milho secos e armazenados em silos com ar natural forçado. Grãos de milho colhidos com 17,8; 18,9 e 20,5% de água foram acondicionados em silos tipo alambrado com fundo falso perfurado e com ventiladores ligados ininterruptamente até a secagem. Amostras de milho foram coletadas nos tempos zero, 4, 8, 22 e 112 dias, em seis alturas de camada de cada silo (10, 60, 110, 160, 210 e 260 cm. A composição química foi determinada pelo uso de Espectrofotômetro do Infravermelho Proximal (NIR e os resultados expressos em porcentagem e em base seca. Os dados foram analisados estatisticamente (Anova e nas interações significativas, elaborados os gráficos de regressão. Nas determinações de proteínas, lipídios, matéria seca e fibra em detergente neutro as interações foram significativas ao nível de 5% de probabilidade de erro, enquanto que em cinzas, umidade e carboidratos não foram verificadas diferenças significativas. A aeração natural forçada é capaz de reduzir a umidade do milho para 13%, sendo tecnicamente viável em propriedades familiares.The aim of this work was to study the effects of moisture at harvest and time of storage upon grains of corn dried and stored in bins with forced natural air. Grains of corn harvested with 17.8; 18.9, and 20.5% of water were stored in drying bins with false floor with fans working full-time until drying. Samples of corn were collected at zero, 4, 8, 22, and 112 days from six different heights in each bin (10, 60, 110, 160, 210 and 260 cm. The chemical composition was determined through Near-Infrared Spectrophotometer (NIR and the results were expressed in percentage at dry basis. The data were statistically analyzed (Anova and for the significant interactions graphics of regression were provided. In the process of determination of protein, fat, dry matter, and neutral detergent fiber the

  4. Análise multivariada de atributos microbiológicos e químicos do solo em florestas com Araucaria angustifolia Multivariate analysis of soil microbiological and chemical attributes in forests with Araucaria angustifolia

    Directory of Open Access Journals (Sweden)

    Dilmar Baretta

    2008-12-01

    Full Text Available Araucaria angustifolia é uma espécie de árvore ameaçada de extinção no Brasil e pouco se conhece sobre os atributos edáficos dessas florestas. Este estudo foi realizado com o objetivo de identificar diferenças entre áreas com araucária naturais e reflorestadas, com base em atributos microbiológicos e químicos do solo, por meio de métodos multivariados, como a análise canônica discriminante (ACD e a análise de correlação canônica (ACC. As áreas estudadas incluem: 1. floresta nativa com araucária (NF; 2. reflorestamento de araucária (R; 3. reflorestamento de araucária submetido a incêndio acidental (RF; e 4. pastagem natural com araucárias nativas e ocorrência de incêndio (NPF. Foram selecionadas, ao acaso, quinze árvores de araucária por área e sob a copa de cada uma delas foram retiradas três amostras de solo, em três épocas contrastantes. A ACD foi aplicada aos atributos microbianos: C da biomassa microbiana (CBM, respiração basal (C-CO2 e quociente metabólico (qCO2, enquanto a ACC foi aplicada aos atributos microbianos e químicos do solo [pH (CaCl2, C orgânico total (COT e teores de P, K, Ca, Mg e (H+Al. Os atributos microbianos e químicos do solo apresentaram alta correlação canônica, com destaque para o CBM e C-CO2, entre os atributos microbianos, e para COT e P, entre os atributos químicos do solo. A ACC mostrou que 52 % da variação total dos atributos microbianos é explicada pela variação dos atributos químicos, e 36 % da variação total dos atributos químicos é explicada pelos atributos microbianos do solo das áreas estudadas. A ACD identificou que a contribuição de cada atributo microbiano para a separação das áreas sofreu variação ao longo do tempo, e discriminou o CBM como sendo o atributo microbiano mais importante, seguido pelo C-CO2. ACC e ACD são importantes ferramentas no estudo de indicadores de qualidade do solo.Araucaria angustifolia is an endangered tree species in

  5. Induction of bacterial antibiotic resistance by mutagenic halogenated nitrogenous disinfection byproducts.

    Science.gov (United States)

    Lv, Lu; Yu, Xin; Xu, Qian; Ye, Chengsong

    2015-10-01

    Halogenated nitrogenous disinfection byproducts (N-DBPs) raise concerns regarding their mutagenicity and carcinogenicity threatening public health. However, environmental consequence of their mutagenicity has received less attention. In this study, the effect of halogenated N-DBPs on bacterial antibiotic resistance (BAR) was investigated. After exposure to bromoacetamide (BAcAm), trichloroacetonitrile (TCAN) or tribromonitromethane (TBNM), the resistance of Pseudomonas aeruginosa PAO1 to both individual and multiple antibiotics (ciprofloxacin, gentamicin, polymyxin B, rifampin, tetracycline, ciprofloxacin + gentamicin and ciprofloxacin + tetracycline) was increased, which was predominantly ascribed to the overexpression of efflux pumps. The mechanism of this effect was demonstrated to be mutagenesis through sequencing and analyzing antibiotic resistance genes. The same induction phenomena also appeared in Escherichia coli, suggesting this effect may be universal to waterborne pathogens. Therefore, more attention should be given to halogenated N-DBPs, as they could increase not only genotoxicological risks but also epidemiological risks of drinking water. PMID:26114900

  6. Mutagenic potential of a 193-nm excimer laser on fibroblasts in tissue culture

    International Nuclear Information System (INIS)

    In response to concerns regarding possible DNA damage by far ultraviolet radiation during excimer laser corneal surgery, the mutagenic potential of an argon fluoride excimer laser (193 nm) on BALB/3T3 mouse fibroblasts grown in tissue culture was investigated. The cumulative incidence of anaplastic transformation after subablative radiant exposures from 3.5 mJ/cm2/pulse to 13.4 mJ/cm2/pulse was 3.6% of all cell cultures. The incidence of anaplastic transformation in nonirradiated controls was 4.2%. Transformation after exposure to x-ray radiation (60.9 rad) was 98.8%. The difference between the incidence of transformation of nonirradiated controls or excimer-treated cultures compared with x-ray radiation-treated cells was significant P less than 0.0001 (chi square test). In this standard cell line, 193-nm laser energy does not appear to have substantial mutagenic potential

  7. Onderzoek naar de mutagene werking van methylmethacrylaat met L5178Y muize-lymfoomcellen

    OpenAIRE

    Knaap, van der, J.A.; A.G.A.C.; Langebroek; P G

    1986-01-01

    Methylmethacrylaat of methacrylzure-methyl-ester bleek geen mutagene werking te hebben in de genmutatie test met L5178Y muize-lymfoomcellen, in afwezigheid van een systeem voor metabole activering (S9), bij concentraties van 3,0 ml/l (28,2 mmol/l) en lager. Ook in aanwezigheid van S9 bij concentraties van 1,5 ml/l (14,1 mmol/l) en lager werd geen mutagene werking gevonden. Cytotoxische effecten werden gevonden vanaf 2,0 ml/l (18,8 mmol/l) zonder S9 en vanaf 1,0 ml/l (9,4 mmol/l) met S9. De on...

  8. Mutagenic and genotoxic potential of direct electric current in Escherichia coli and Salmonella thyphimurium strains.

    Science.gov (United States)

    Gomes, Marina das Neves; Cardoso, Janine Simas; Leitão, Alvaro Costa; Quaresma, Carla Holandino

    2016-05-01

    Direct electric current has several therapeutic uses such as antibacterial and antiprotozoal action, tissues scarring and regeneration, as well as tumor treatment. This method has shown promising results in vivo and in vitro, with significant efficacy and almost no side effects. Considering lack of studies regarding direct electric current mutagenic and/or genotoxic effects, the present work evaluated both aspects by using five different bacterial experimental assays: survival of repair-deficient mutants, Salmonella-histidine reversion mutagenesis (Ames test), forward mutations to rifampicin resistance, phage reactivation, and lysogenic induction. In these experimental conditions, cells were submitted to an approach that allows evaluation of anodic, cathodic, and electro-ionic effects generated by 2 mA of direct electric current, with doses ranging from 0.36 to 3.60 Coulombs. Our results showed these doses did not induce mutagenic or genotoxic effects. Bioelectromagnetics. 37:234-243, 2016. © 2016 Wiley Periodicals, Inc. PMID:27018544

  9. Mutagenic activity of some coal-derived humic compounds evaluated by the Ames test.

    Science.gov (United States)

    Bernacchi, F; Ponzanelli, I; Barale, R; Loprieno, N

    1996-07-10

    Two coal-derived humic substances (Sulcis and South Africa, Eniricerche, Italy) have been evaluated for their mutagenic activity on TA98 and TA100 Salmonella typhimurium strains, either in presence or in absence of metabolic activation (S9). Both compounds showed no effect on the two strains, as observed with natural humic acid (Fluka). After chlorination, coal-derived humic acids induced a strong dose-related increase in the number of revertants on TA100 without S9, whose extent was directly proportional to the chlorination ratios. Such effect was completely suppressed when a sodium thiosulfate solution (10%) was added at the end of the chlorination period (about 90 h). The analogies with natural humic acid mutagenicity are discussed. PMID:8700175

  10. Mutagenic activity of some coal-derived humic compunds evaluated by the Ames test

    Energy Technology Data Exchange (ETDEWEB)

    Bernacchi, F.; Ponzanelli, I.; Barale, R.; Loprieno, N. [Universita degli Studi di Pisa, Pisa (Italy). Dipt. Scienze dell`Ambiente e del Territorilo

    1996-07-10

    Two coal-derived humic substances were evaluated for their mutagenic activity on TA98 and TA100 Salmonella typhimurium strains, either in the presence or absence of metabolic activation (S9). Both compounds showed no effect on the two strains, as observed with natural humic acid (Fluka). After chlorination, coal-derived humic acids induced a strong dose-related increase in the number of revertants on TA100 without S9, whose extent was directly proportional to the chlorination ratios. Such effect was completely suppressed when a sodium thiosulphate solution (10%) was added at the end of the chlorination period (about 90 h). The analogies with natural humic acid mutagenicity are discussed. 19 refs., 5 tabs.

  11. Influence of mutagenic factors on the epidermis of tomatillo, currant tomato and tomato

    International Nuclear Information System (INIS)

    Epidermal features of control, 16 treated and 7 mutants of Physalis ixocarpa BROT. (tomatillo), Lycopersicon esculentum MILL. (tomato) and L. pimpinellifolium MILL. (currant tomato) were studied. Although the distribution, ontogenesis and mature structure of stomata in control as well as in treated and mutant plants were similar, the mutagens reduced the stomatal abnormalities which were abundant (60%) in P. ixocarpa. Maximum rectification was found after combined treatment with gamma radiation (5.16 C kg-1 [=20 kR]) +1% dimethyl sulfate. Methyl ethanesulfonate, gamma radiation and gamma radiation + DMS caused an increase in epidermal cell size irrespective of the leaf size but in DMS and diethyl sulfate treated plants, a close negative correlation between leaf size and epidermal cell size was observed. The size and frequency of stomata were also affected, the large leaf usually showed high frequency and small size stomata. The data reveal that mutagens affect the epidermal structures differently. (author)

  12. City air pollution of polycyclic aromatic hydrocarbons and other mutagens: occurrence, sources and health effects

    DEFF Research Database (Denmark)

    Nielsen, T.; Ejsing Jørgensen, Hans; Larsen, J.C.;

    1996-01-01

    The presence of polycyclic aromatic hydrocarbons (PAH), mutagens and other air pollutants was investigated in a busy street in central Copenhagen and in a park area adjacent to the street. The winter concentration of benzo(a)pyrene was 4.4+/-1.2 ng/m(3) in the street air and 1.4+/-0.6 ng/m(3) in...... the city park. The atmospheric concentrations of PAH decreased in the order of: street > city background air similar to suburbs > village > open land. The traffic contribution of PAH to street air was estimated to be 90% on working days and 60% during weekends and its contribution to city background...... air was estimated to be 40%. Four different approaches to evaluate the health effects are discussed. The direct effect of PAH air pollution, and other mutagens, is considered to be a maximum of five lung cancer cases each year out of one million people....

  13. The lethal and mutagenic action of solar radiation on the model microbiological test-systems

    International Nuclear Information System (INIS)

    Using model microbiological ob ects - phage T4, Escherichia coli bacterii and Aspergillus nidulans fUngUs-ascomycetis, lethal mutagenous and recombinogenous effect of solar radiation are studied. Considerable lethal effect of solar radiation on T4 phage and E. coli and the increase in the mUtation frequency in E. coli and A. nidUlans are observed. Recombination effect of solar radiation is demonstrated on diploid strain of A. nidulans. It is shown that systems of excision and post-replicative reparation take part in the correction of injUries induced by solar light. A considerable role of ultraviolet range (260-320 nm) of solar radiation in the induction of lethal and mutageneous effects in all objects investigated is found

  14. Analysis of mutagenic effects induced by carbon beams at different LET in a red yeast strain

    International Nuclear Information System (INIS)

    To evaluate inactive and mutagenic effects of carbon beam at different LET, the inactivation cross section and mutation cross section induced by carbon beams of different LET values were investigated in a red yeast strain Rhodotorula glutinis AY 91015. It was found that the maximum inactivation cross section of 4.37μm2, which was very close to the average nucleus cross section, was at LET of 120.0 keV/μm. The maximum mutation cross section was at LET of 96.0 keV/μm. Meanwhile, the highest mutagenicity of carbon ion was found around 58.2 keV/μm. It implied that the most efficient LET to induce mutation in survival yeasts was 58.2 keV/μm, which corresponded to energy of 35 MeV/u carbon beam. The most effective carbon beam to induce inactivation and mutation located at different energy region. (authors)

  15. Hygienic quality and mutagenicity of minced meats and patties treated by ionizing energy

    International Nuclear Information System (INIS)

    This study was conducted to evaluate the microbial safety of minced beef, minced pork, beef patties and pork patties irradiated with gamma ray or electron beam at an absorbed dose from 5 to 20 kGy. Also, the mutagenicity of minced beef, minced pork and patties treated with irradiation at 50 kGy was evaluated by Ames test (Salmonella typhimurium reversion assay). The results of the total aerobic bacteria of the minced beef, minced pork and patties showed that the sterilization effect of gamma irradiation was superior to that of electron beam irradiation. The results from Ames test showed that all samples were negative in the bacterial reversion assay with S. typhimurium TA98 and TA100. Also, no mutagenicity was detected in the assay, both with and without metabolic activation

  16. Mutagenic action of γ-rays on isolated plasmid DNA of Escherichia Coli

    International Nuclear Information System (INIS)

    A purified and drief DNA of plasmid pKO482 (galK+) is 10 times more resistant to the inactivating action of 60Co-γ-rays than that of lambda phage. γ-irradiation of the plasmid DNA induces forward mutations of galK, the frequency of which increases linearly with the dose. The efficiency of the mutagenic action of γ-rays on the plasmid galK locus is 10-12 per 1 rad and per 1 base pair. The mutagenic effect of γ-M radiation but slightly depends upon bacterial recA+ gene and and upon the SOS-repair system induced by UV-irradiation of the recipient cells. It is assumed that the premutational lesions induced in the purified DNA by the direct effect of γ-radiation are fixed into mutations by misreplication

  17. Mutagenic effects of chromium trioxide on root tip cells of Vicia faba

    Institute of Scientific and Technical Information of China (English)

    钱晓薇

    2004-01-01

    In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO3) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus rate and chromosome aberration rate of Viciafaba root tip cells. The results showed that the effects of CrO3 concentration on the mitotic indexes were complicated. CrO3 increases the micronucleus rate of Vicia faba root tip cells. It was found that within certain range of CrO3 concentration the micronucleus rate increased systematically with increased concentration of CrO3, but that the micronucleus rate decreased at higher level of CrO3 and that CrO3 also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO3. We concluded that CrO3 has significant mutagenic effect on Viciafaba root tip cells.

  18. Mutagenic effects of chromium trioxide on root tip cells of Vicia faba

    Institute of Scientific and Technical Information of China (English)

    钱晓徽

    2004-01-01

    In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO3) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus rate and chromosome aberration rate of Vicia faba root tip cells. The results showed that the effects of CrO3 concentration on the mitotic indexes were complicated. CrO3 increases the micronucleus rate of Vicia faba root tip cells. It was found that within certain range of CrO3 concentration the micronucleus rate increased systematically with increased concentration of CrO3, but that the micronucleus rate decreased at higher level of CrO3 and that CrO3 also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO3. We concluded that CrO3 has significant mutagenic effect on Vicia faba root tip cells.

  19. Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

    OpenAIRE

    Chandrasekaran Chinampudur Velusami; Srinivasa Rao Boddapati; Srikanth Hongasandra Srinivasa; Edwin Jothie Richard; Joshua Allan Joseph; Murali Balasubramanian; Amit Agarwal

    2013-01-01

    Curcuma longa Linn. (Zingiberaceae) commonly known as turmeric has long been used for centuries as a spice and household remedy. The present study was carried out to assess the possible mutagenic potential and acute oral toxicity of polysaccharide extract of turmeric rhizome (NR-INF-02) using standard tests. The standard battery of in vitro genotoxicity tests, bacterial reverse mutation test (BRMT), chromosome aberration (CA), and micronucleus (MN) tests were employed to assess the possible m...

  20. Polyamines stimulate the formation of mutagenic 1,N 2-propanodeoxyguanosine adducts from acetaldehyde

    OpenAIRE

    Theruvathu, Jacob A.; Jaruga, Pawel; Nath, Raghu G.; Dizdaroglu, Miral; Brooks, P. J.

    2005-01-01

    Alcoholic beverage consumption is associated with an increased risk of upper gastrointestinal cancer. Acetaldehyde (AA), the first metabolite of ethanol, is a suspected human carcinogen, but the molecular mechanisms underlying AA carcinogenicity are unclear. In this work, we tested the hypothesis that polyamines could facilitate the formation of mutagenic α-methyl-γ-hydroxy-1,N 2-propano-2′-deoxyguanosine (Cr-PdG) adducts from biologically relevant AA concentrations. We found that Cr-PdG addu...