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Sample records for cervical precancerous lesions

  1. Predrakave spremembe materničnega vratu: Cervical precancerous lesions:

    OpenAIRE

    Jančar, Nina; Vrtačnik-Bokal, Eda

    2010-01-01

    Cervical cancer evolves through several stages of precancerous lesions and can therefore be prevented by me ans of organized screening program and effective treatment of precancerous lesions. Persistent infection with high-risk or oncogenic human papillomavirus genotypes has been accepted as thesingle necessary etiological factor for cervical precancerous lesions and cervi cal cancer. Histologicaly we divide cervical squamous cel1 precancerous lesions into three grad es; cervi cal intraepithe...

  2. Factors influencing decisions about surgical treatment of cervical precancerous lesions.

    Science.gov (United States)

    Sehnal, Borek; Cibula, David; Slama, Jiri

    2014-04-01

    Cervical precancerous lesions represented by cervical intraepithelial neoplasia (CIN) and cervical glandular intraepithelial neoplasia may progress to invasive cancer. The principle treatment of CIN is eradication of the transformation zone. However, all eradication methods are associated with some adverse events, particularly with perinatal consequences. It is therefore necessary to identify which women have CIN that has a low risk of transformation into invasive cancer. The presence of modifying factors can help to stratify CIN lesions according to their malignant potential. The evaluation of HPV genotype in particular holds great promise for defining patients at greater risk. Tailoring treatment to the individual patient is going to become a major consideration in the management of cervical precancerous lesions.

  3. Etiology and pathogenesis of precancerous lesions and invasive cervical carcinoma

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    Panjković Milana

    2008-01-01

    Full Text Available Cervical cancer is the second most common gynecological malignancy in the world. Human papilloma virus (HPV infection is the leading ethiologic agent in the development of premalignant and malignant cervical diseases. HPV is a member of the Papovaviridae family and until now over 100 types have been recognized. There are two types of viral infection: latent and productive. Virus induced oncogenesis is the result of interaction between virus oncoproteins E6 and E7 and tumor supresor host genes p53 and Rb. Many cofactors such as immunosuppression, early sexual relationship, multiple sexual partners, other sexualy transsmited infections and smooking are contributing factors of the precancerous and invasive cervical lesions. According to the oncogenic potential HPV are divided into three groups: low, intermediate and high oncogenic risk viruses. Molecular technics which are used for the virus detection are: In situ hibridisation,, Hybrid capture test and polymerasa chain reaction. Human papilloma virus testing has an important role in the follow up and treatment of women with 'atypical squamous cells of unknown significant' changes in cervical smears and low-grade squamous intraepithelial lesions, changes in punch biopsy.

  4. Prevalence and Risk Factors Associated with Precancerous Cervical Cancer Lesions among HIV-Infected Women in Resource-Limited Settings

    Directory of Open Access Journals (Sweden)

    Peter Memiah

    2012-01-01

    Full Text Available Objective. To assess the prevalence and identified associated risk factors for precancerous cervical cancer lesions among HIV-infected women in resource-limited settings in Kenya. Methods. HIV-infected women attending the ART clinic at the Nazareth Hospital ART clinic between June 2009 and September 2010. Multivariate logistic regression model with odds ratios and 95% confidence intervals (CI were estimated after controlling for important covariates. Result. A total of 715 women were screened for cervical cancer. The median age of the participants was 40 years (range 18–69 years. The prevalence of precancerous lesions (CINI, CINII, CIN III, ICC was 191 (26.7%. After controlling for other variables in logistic regression analysis, cervical precancerous lesions were associated with not being on ART therapy; whereby non-ART were 2.21 times more likely to have precancerous lesions than ART patients [(aOR=2.21, 95% CI (1.28–3.83]. Conclusion. The prevalence of precancerous cervical lesions was lower than other similar settings. It is recommended that cancer screening of HIV-infected women should be an established practice. Availability and accessibility of these services can be done through their integration into HIV. Prompt initiation of HAART through an early enrollment into care has an impact on reducing the prevalence and progression of cervical precancerous lesions.

  5. The prevalence of precancerous cervical cancer lesion among HIV-infected women in southern Ethiopia: a cross-sectional study.

    Directory of Open Access Journals (Sweden)

    Abel Gedefaw

    Full Text Available INTRODUCTION: The magnitude of precancerous cervical cancer lesions as well as invasive cervical cancer is higher in HIV-infected women than non HIV-infected women. Thus, screening targeting HIV-infected women is being undertaken in developing countries, including Ethiopia. However, data on the prevalence and determinants of precancerous cervical cancer lesion among HIV-infected women in southern Ethiopia is lacking. Thus, this study aimed to assess the prevalence of and factors associated with precancerous cervical cancer lesion among HIV- infected women in southern Ethiopia. METHODS: A hospital-based cross-sectional study was conducted from October 2012 to February 2013 among HIV-infected women in Southern Ethiopia. Four hundred forty eight HIV-infected women who had been screened and treated for precancerous cervical cancer lesion were included in the study. Data were collected by using structured and pretested questionnaire. Visual inspection with acetic acid was applied for screening and treatment. SPSS version 16.0 was used for data entry and analysis. Logistic regression analysis was fitted and odds ratios with 95% Confidence intervals and p-values were computed to identify factors associated with precancerous cervical cancer lesion. RESULTS: Out of 448 study participants, 99 (22.1% were found to be positive for precancerous cervical cancer. Being currently on highly active antiretroviral treatment (AOR=0.52, 95%CI: 0.35, 0.92, history of sexually transmitted disease (AOR=2.30, 95%CI: 1.23, 4.29 and having only one lifetime sexual partner (AOR=0.33, 95%CI: 0.20, 0.56 were factors associated with precancerous cervical cancer lesion. CONCLUSIONS: The prevalence of precancerous cervical cancer lesion among HIV-infected women in southern Ethiopia was found to be high. Intervention to access all HIV-infected women like scaling up the limited services and awareness creation should be undertaken. Measures aimed at preventing the acquisition and

  6. Expression and role of AQP1 in cervical squamous carcinoma and its precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective: To investigate the expression of aquaporin 1 in cervical squamous carcinomas (CSC) and cervical precancerous lesions, and the relationship between the tumor clinicopathological parameters, prognosis and the expression of AQP1. Methods: Immunohistochemical method (EliVision) was used to detect the expression of AQP1 in samples from 106 patients [20 with normal cervical tissue, 30 with cervical intraepithelial neoplasia (stage Ⅰ and Ⅱ) and 56 with CSC]. Survival analysis was performed by Kaplan-Meier method. Results: AQP1 protein was expressed in vascular endothelia of all samples. It showed upregulation of AQP1 expression in CSC. There was a significant difference between CSC and normal cervical tissues (P<0.05). AQP1 was expressed in some tumor cells and unexpressed in normal squamous epithelial cells. And APQ1-expressing tumor cells were positively related to lymph node metastasis. Patients with APQ1-expressing tumor cells had the lower survival rate than the ones without. Conclusion: Abnormal expression of AQP1 plays an important role in the development of CSC. Positive expression of AQP1 in tumor cells maybe enhances tumor metastasis and could be used as a marker for tumor prognosis.

  7. Prevalence of human papillomavirus types in cervical cancerous and precancerous lesions of Ecuadorian women.

    Science.gov (United States)

    Mejía, Lorena; Muñoz, Diana; Trueba, Gabriel; Tinoco, Leopoldo; Zapata, Sonia

    2016-01-01

    Human Papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it is responsible for most cases of uterine cancer. In Ecuador there is limited information about HPV types (and variants) in cancerous lesions; however, identifying the type-specific HPV prevalence in cervical lesions of women living in Ecuador is important to better predict the impact of HPV prophylactic vaccination in this country. We studied the prevalence of HPV types in cervical cancerous or precancerous lesions from 164 Ecuadorian women and found that 86.0% were HPV positive. The most common types were HPV16 (41.8%) and HPV58 (30.5%). Interestingly, HPV18 was detected only in 2.8% of the HPV-positive samples. Fifteen DNA sequences (genes E6 and L1) from 16 samples positive for HPV16 belonged to the European lineage, considered one of the least carcinogenic lineages, and 1 (6.25%) to the Asian-American lineage. Similar analysis in 12 HPV58 positive samples showed that 10 (83.3%) sequences grouped in sublineage A2, which belongs to the oldest HPV58 lineage, 1 belonged to A3 and 1 to lineage C. This study suggests that the currently used HPV vaccines (bivalent and tetravalent) may have lower effectiveness in Ecuador than in other geographic locations where HPV18 is more prevalent.

  8. Relationship between hTERC gene and precancerous lesion as well as occurrence and development of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Li-Hong Yi

    2016-01-01

    Objective:To study the relationship betweenhTERC gene and precancerous lesion as well as occurrence and development of cervical cancer.Methods:A total of 120 cases receiving cervical biopsy in our hospital from May 2012 to December 2014 were selected for study, 45 cases of normal cervical tissue and inflammatory cervical tissue, 37 cases of cervical intraepithelial neoplasia tissue and 38 cases of cervical cancer tissue were included.hTERC gene expression, oncogene expression and invasive molecule contents in cervical tissue were detected.Results:Green signal copy number and red signal copy number ofhTERC in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue, and the contents of hTERTand hTP were not different from those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue with high expression of hTERC were significantly higher than those in cervical cancer tissue with low expression of hTERC.Conclusions:Activation of telomerase and increase of hTERC expression are involved in the precancerous lesion as well as the occurrence and development of cervical cancer, and hTERC can activate the expression of oncogenes and.

  9. Reliability of the CINtecTM p16INK4a immunocytochemical test in screening cervical precancerous lesions

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    Jović Milena

    2008-01-01

    Full Text Available Background/Aim. Overexpression of p16INK4a has been found to be linked with genomic integration of high-risk human papillomavirus (HPV and the developement of precancerous cervical intraepithelial lesions. The aim of this study was to examine is there a higher positive level of correlation between grade of histological dysplasia and p16INK4a level of expression in cervical smear, compared to results of Papanicolaou test. We also examined the correlation between HPV type, p16INK4a expression and Papanicolau test results. Methods. A total of 48 women with precanceorous cervical lesions and HPV cervicitis and 10 healthy women were enrolled in the study. Papanicolaou test, CINtecTM p16INK4a citological immunohistochemical test, polymerase chain reaction (PCR HPV 16, 18, 31, 33 analysis and histopathology of the lesion were performed in all the patients. Results. Comparing the results of Papanicoulaou test and the grade of histological dysplasia, low-grade squamous intraepithelial lesion (LSIL was confirmed in 38%, and high-grade squamous intraepithelial lesion (HSIL in 69.2% of the patients (p > 0.05. Significant positive correlation was found between p16 overexpression and grade of histological dysplasia (p = 0.000. Overexpression p16 was found in 70% of LSIL and 94.4% of HSIL. Positive correlation was found between p16 overexpression and grade of dysplasia in Papanicolaou test (p = 0.011. In 38% of LSIL and 15% of HSIL cases p16 was not expressed. The most frequently found HPV type in PCR analysis was HPV16. Analysing the results of p16 test according to HPV status and Papanicolaou test rather heterogenous results were obtained. Conclusion. In the patients with precancerous cervical lesions a higher level of correlation was found between the grade of histological dysplasia and p16INK4a level of expression in the cervical smear, compared to the results of Papanicolaou test.

  10. Clinical significance of HPV-DNA testing for precancerous cervical lesionS

    OpenAIRE

    Moarcăs, M; Georgescu, IC; Brătilă, E; Badea, M.; Cîrstoiu, ECM

    2014-01-01

    Cervical screening by using cytology was proven efficient in reducing the mortality secondary to cervical cancer, but this method has limitations. High risk HPV infection is essential for cervical cancer development so HPV testing is a new tool used for screening patients for cervical neoplasia. HPV testing was proven most useful for women over 30 years old, in cases in which cytology identified ASC-US and after treatment for CIN. This article outlines the clinical significance of HPV-DNA tes...

  11. Clinical and evolving features of women diagnosed with precancerous cervical lesions, screened and treated in the Amazon region of Brazil.

    Science.gov (United States)

    Prado, Patricia Rezende do; Koifman, Rosalina Jorge; Silva, Ilce Ferreira da

    2014-09-01

    The objective of the study was to determine the dynamics of precancerous lesions in women of a cohort treated for cervical intraepithelial neoplasia (CIN) and followed up over the next two years. The conditional probability of failure was calculated using the Kaplan-Meier method, and the raw and adjusted hazard ratios (HR) were determined using Cox regression with a p-value entry of history of more frequent pregnancies and a histological diagnosis of CIN II/III are directly correlated with risk of CIN treatment failure, whereas being in a stable relationship is inversely correlated with this risk.

  12. Clinical and evolving features of women diagnosed with precancerous cervical lesions, screened and treated in the Amazon region of Brazil

    Directory of Open Access Journals (Sweden)

    Patricia Rezende do Prado

    2014-09-01

    Full Text Available The objective of the study was to determine the dynamics of precancerous lesions in women of a cohort treated for cervical intraepithelial neoplasia (CIN and followed up over the next two years. The conditional probability of failure was calculated using the Kaplan-Meier method, and the raw and adjusted hazard ratios (HR were determined using Cox regression with a p-value entry of < 0.05. Of the 237 women who were treated, 51.5% were accompanied over 24 months, and treatment failed for 21.9% of those accompanied. Women who had five or more pregnancies (adjusted HR = 3.10, 95%CI: 1.28-7.51 or an initial histological diagnosis of CIN II/III demonstrated an independent risk of treatment failure (adjusted HR = 3.14, 95%CI: 1.20-8.19. Being in a stable relationship was a protective factor against treatment failure (adjusted HR = 0.47, 95%CI: 0.24-0.89. A history of more frequent pregnancies and a histological diagnosis of CIN II/III are directly correlated with risk of CIN treatment failure, whereas being in a stable relationship is inversely correlated with this risk.

  13. Trends in the incidence of cervical cancer and severe precancerous lesions in Denmark, 1997-2012

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Munk, Christian; Nielsen, Thor Schütt Svane;

    2015-01-01

    PURPOSE: The incidence of cervical cancer, including squamous cell carcinoma (SCC), has been decreasing in several developed countries since the onset of organized screening programs; in some countries, however, the incidence of adenocarcinoma has increased among young women. We investigated...... the Danish incidence trends during 1997-2011 when cervical screening coverage was high. Incidences of cervical intraepithelial neoplasia grade 3 (CIN3) and adenocarcinoma in situ (AIS) were also assessed, with the latest part of the study period coinciding with introduction of free-of-charge human......, importantly, they decreased significantly during 2009-2012 in women aged ≤20 years. CONCLUSIONS: The Danish screening program has successfully reduced the incidence of cervical cancer, especially of SCC in older women; however, the program has not significantly reduced the incidence in young women...

  14. Meta-analysis of type-specific human papillomavirus prevalence in Iranian women with normal cytology, precancerous cervical lesions and invasive cervical cancer: Implications for screening and vaccination.

    Science.gov (United States)

    Jalilvand, Somayeh; Shoja, Zabihollah; Nourijelyani, Keramat; Tohidi, Hamid Reza; Hamkar, Rasool

    2015-02-01

    To predict the impact of current vaccines on cervical cancer and for the improvement of screening programs, regional data on distribution of human papillomavirus (HPV) types in women with or without cervical cancer is crucial. The present meta-analysis intend to comprehensively evaluate the HPV burden in women with invasive cervical cancer, high-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 2 and 3), low-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 1), atypical squamous cells of undetermined significance and normal cytology, as these data will help decision making in regards with screening programs and HPV vaccination in Iran. To determine the HPV prevalence and type distribution in Iranian women with or without cervical cancer, 20 published studies were included in this meta-analysis. In total, 713, 124, 104, 60, and 2577 women invasive cervical cancer, high-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 2 and 3), low-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 1), atypical squamous cells of undetermined significance and normal were reviewed, respectively. Overall HPV prevalence in women with invasive cervical cancer, high-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 2 and 3), low-grade squamous intraepithelial lesion (cervical intraepithelial neoplasia 1), atypical squamous cells of undetermined significance and normal cytology were 77.4%, 71.8%, 65.3%, 61.7%, and 8.4%, respectively. The six most common types were HPV 16, 18, 6/11, 31, and 33; among them HPV 16 was the most frequent type in all five different groups. According to this study, it was estimated that HPV vaccines could have a great impact on prevention of cervical cancer in Iran. In conclusion, this meta-analysis highlights the necessity of introducing vaccination program in Iran.

  15. 超声弹性成像对宫颈癌前病变和宫颈癌的诊断价值%Diagnostic Value of Ultrasound Elastography for Cervical Precancerous Lesions and Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    粟碧蓉

    2016-01-01

    目的:研究分析超声弹性成像在诊断宫颈癌前病变与宫颈癌中的应用价值。方法选取2014年9月至2015年手术确诊并治疗的宫颈癌前病变与宫颈癌患者56例(观察组)和同期无宫颈癌前病变与宫颈癌健康者22例为研究对象(对照组)。在观察组手术治疗前借助超声弹性成像对宫颈病灶进行检查,将弹性分级与术后分期进行对比。结果与对照组相比较,观察组患者超声弹性成像的内容并不一致,在弹性分级1~3级检出率中,宫颈癌前病变要高于宫颈癌,而弹性分级4~5级的检出率,宫颈癌要显著高于宫颈癌前病变,数据符合统计学差异(P <0.05);在弹性分级不超过4级的诊断中,宫颈癌的弹性分级诊断准确29例,准确率为93.5%,宫颈癌前病变弹性分级诊断准确18例,准确率为72%,数据符合统计学差异(P <0.05);超声弹性诊断宫颈癌前病变与宫颈癌准确率为83.9%。结论临床诊断宫颈癌前病变与宫颈癌的时候,超声弹性成像技术具有较高的应用价值,可在临床上推广应用。%Objective To study the value of ultrasound imaging in the diagnosis of cervical precancerous lesions and cervical cancer. Methods In September 2014 to 2015 surgical diagnosis and treatment of cervical cancer, precancerous lesions and cervical cancer patients of 56 cases (observation group) and compared with no health cervical cancer, precancerous lesions and cervical cancer 22 cases as the object of study (control group) were selected. The cervical lesions were examined by ultrasound before surgery in the observation group, and the elastic grading was compared with the postoperative stage. Results Compared with control group, the observation group of patients with ultrasound elasticity imaging is not consistent in graded 1-3 level detection rate in cervical precancerous lesion is higher than that of cervical cancer, and elastic grade 4

  16. 宫颈癌及癌前病变相关危险因素分析%The risk factors of cervical cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    盖俊峰; 陈晓军; 寇明捷; 毛莉

    2014-01-01

    目的:对宫颈癌及癌前病变的相关危险因素进行分析研究,为相关理论研究提供信息。方法随机选取我院2010年6月至2013年6月经临床筛查确诊为宫颈癌患者30例、宫颈癌前病变(CIN)患者50例和健康查体者50名作为研究对象,宫颈癌和癌前病变患者共80例列入病变组,健康查体者50名列入对照组,比较两组研究对象人口统计学资料、生育史、性生活等情况。结果病变组与对照组各项危险因素:人乳头瘤病毒(HPV)感染阳性率分别为92.5%和4.0%(χ2=99.239,P 45岁比例分别为35.0%和16.0%(χ2=5.547,P 45 years in two group were 35. 0% ane 16. 0%(χ2 = 5. 547,P < 0. 05). The rate of eeucation below high school were 17. 5% ane 36. 0%(χ2 = 5. 675,P < 0. 05). Sexual perioes ≥ 3 years were 90. 0% ane 72. 0%(χ2 = 7. 090,P < 0. 01). The rate of proeuction times ≥ 2 times were 37. 5% ane 20. 0%(χ2 = 4. 423,P < 0. 05). The abortion rates ≥ 2 times were 27. 5% ane 12. 0%(χ2 = 4. 374,P < 0. 05). The non-contraception rates were 87. 5% ane 72. 0%(χ2 = 4. 910,P < 0. 05). Un-regular examination rates were 77. 5% ane 56. 0%( χ2 = 6. 676,P < 0. 01). There were significant eifferences between the relevant eata.Conclusion The factors inclueing HPV infection,age,eeucation level,sex,proeuction rate,number of abortions,contraception,ane un-regularly checkee are the risk factors of cervical precancerous lesions. The approaches such as women′ s regular gynecological examination,early cancer screening ane eiscovery precancerous lesions were provee to improve early intervention ane prevention of cervical cancer.

  17. Study to assess the acceptability and feasibility of cervical cancer screening using visual inspection with acetic acid and treatment of precancerous lesions using cryotherapy in low resource settings

    Directory of Open Access Journals (Sweden)

    Tosha M. Sheth

    2014-06-01

    Full Text Available Background: Objective of current study was to assess the acceptability and feasibility of cervical cancer screening using VIA, and treatment of precancerous lesions using cryotherapy in low resource settings Methods: 526 women from three primary health centers of Shinor taluka (population based approach and 250 women of Medical College, Vadodara (facility based approach were sensitized and screened for cervical cancer. Visual Inspection with Acetic acid (VIA was performed as the screening test. In the population based approach, VIA positive women were referred to a Community Health Centre (CHC for colposcopy and biopsy. Ablative treatment in the form of cryotherapy was offered. Patients requiring higher forms of treatment were referred to medical college, Vadodara. In the facility based approach, VIA positive women underwent colposcopy. Guided biopsy was performed in those with positive lesions on colposcopy. Cryotherapy was offered in the same sitting. Those not suitable for cryotherapy were offered loop electrosurgical excision procedure. Women found to have invasive cancer were offered definitive management. Results: VIA positivity rate was 18.8% in the population based approach and 27.2% in the facility based approach.58.8% women in the population based approach and 77.77% women in the facility based approach were treated with cryotherapy on the same day as screening and none reported any severe side effects. Dropout rate in the community approach was 32.32% whereas in the facility it was 0.4%. Conclusions: VIA and cryotherapy procedures were well tolerated by all screened women. This project has shown that the and ldquo;screen and treat and rdquo; approach can be successfully implemented in the existing health setup. [Int J Reprod Contracept Obstet Gynecol 2014; 3(3.000: 615-621

  18. Precancerous Lesions of the Oral Mucosa

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    Oya Gürbüz

    2012-12-01

    Full Text Available In this review of oral precancerous lesions, leukoplakia, erythroleukoplakia/erythroplakia and the least common variant proliferative verrucous leukoplakia will be focused with their clinical characteristics and their potential to develop oral squamous cell carcinoma and related factors will be discussed.

  19. 薄层液基细胞学在宫颈癌及其癌前病变筛查中的价值%The value of Thin prep cytology test in cervical precancerous lesions and cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    雷静; 万霖; 李惠新; 蒋瑛; 李瑞萍

    2011-01-01

    Objective: To evaluate the detecting technology of thin layers of liquid base cytology (TCT) on the diagnosis of precancerous lesions of uterine cervix and the clinical value, accuracy of the cervical cancer screening. Methods: To collect and analysis 7340 patients who were did examine of TCT in gynecological clinic line of our hospital from May, 2009 ~ November, 2010. Take cytology diagnosed ASC - US and higher as positive results, and the positive results do pathohistological. Take histological diagnosis as gold standard. Results: Thin prep cytology test specimens of SCC, and satisfaction LSIL accuracy HSIL, respectively, 76.8% 97.3%, 100%. Conclusion: TCT combination diagnostic systems of TBS are ideal method of current diagnosis precancerous lesions of uterine cervix (CIN) and cervical cancer screening. Also it can be used as an indicator for cervical cancer detecting. There is some risk of precancerous lesions of young In ASC-US patients.%目的:评价薄层液基细胞学(Thin prep cytology test,TCT)检测技术对宫颈癌前病变的诊断和宫颈癌筛查的准确性及临床价值.方法:收集分析2009年5月~2010年11月在我院妇科门诊行TCT检查的受检者7340例,以细胞学诊断为未明确意义的不典型鳞状上皮细胞(ASC-US)及以上者为阳性结果,并对阳性结果行病理组织学诊断,以组织学诊断作为金标准.结果:液基细胞学标本满意度高,对SCC、HSIL、LSIL的准确率分别为76.8%、97.3%、100%.结论:TCT结合TBS诊断系统是目前诊断宫颈癌前病变和筛查宫颈癌的理想方法 [1],同时也可以作为一项宫颈癌术后随访的检测指标 [2].ASC-US患者中存在部分年轻的高危癌前病变者.

  20. Risk of Preterm Delivery Associated with Prior Treatment of Cervical Precancerous Lesion according to the Depth of the Cone

    Science.gov (United States)

    Berretta, Roberto; Gizzo, Salvatore; Dall'Asta, Andrea; Mazzone, Eleonora; Monica, Michela; Franchi, Laura; Peri, Francesca; Patrelli, Tito Silvio; Bacchi Modena, Alberto

    2013-01-01

    The aim of this study was to evaluate the impact of the surgical excisional procedures for cervical intraepithelial neoplasia (CIN) treatment both on subsequent fertility (cervical factor) and pregnancy complication (risk of spontaneous preterm delivery). We retrospectively analyzed 236 fertile women who underwent conization for CIN. We included in the study 47 patients who carried on pregnancy and delivered a viable fetus. Patients were asked about postconization pregnancies, obstetrical outcomes, and a possible diagnosis of secondary infertility caused by cervical stenosis. We evaluated the depth of surgical excision, the timing between cervical conization and subsequent pregnancies, surgical technique, and maternal age at delivery. We recorded 47 deliveries, 10 cases of preterm delivery; 8 of them were spontaneous. The depth of surgical excision showed a statistically significant inverse correlation with gestational age at birth. The risk of spontaneous preterm delivery increased when conization depth exceeded a cut-off value of 1.5 cm. Our data do not demonstrated a relation between conization and infertility due to cervical stenosis. PMID:24324288

  1. Comparison of cervical cell morphology using two different cytology techniques for early detection of pre-cancerous lesions.

    Science.gov (United States)

    Moosa, Najla Yussuf; Khattak, Nuzhat; Alam, Muhammad Irfan; Sher, Alam; Shah, Walayat; Mobashar, Shumaila; Alam, Muhammad Imran; Javid, Asima

    2014-01-01

    Cervical cancer is an issue of foremost importance globally, specifically affecting the developing nations. Significant advances have taken place with regard to diagnosis of cervical cancer, especially with screening. Appropriate screening measures can thus reduce the incidence of cervical cancer. The most desirable screening technique should be less invasive, easy to perform, cost-effective and cover a wide range of diagnostic icons. Manual liquid based cytology (MLBC) can be considered as one of the suitable technique for screening with the above-mentioned benefits. The aim of the current study was to compare two cervical screening techniques on the basis of different morphological parameters and staining parameters by using modified acetic acid Pap staining to see the possibility of reducing time economy involved in conventional Pap staining (CPS). The study was conducted on a total 88 cases and all were analyzed with both MLBC and CPS. Forty eight cases that were regarded as satisfactory on the basis of Bethesda system by both methods were further recruited for investigation. Their morphological parameters and staining quality were compared and scored according to a scoring system defined in the study. Quality indices was calculated for both staining procedures and smear techniques.

  2. 鳞癌抗原在宫颈癌前病变及早期宫颈癌治疗中的价值%Value of squamous cell carcinoma antigen in cervical precancerous lesions and early cervical cancer therapy

    Institute of Scientific and Technical Information of China (English)

    王斌; 张月桃; 李琼珍

    2016-01-01

    目的:探讨鳞癌抗原在宫颈癌前病变及早期宫颈癌治疗中的价值。方法采用酶联接免疫吸附剂测定(ELISA)法初检,化学发光法复检测定宫颈上皮内瘤变(CINⅡ或 CINⅢ)、宫颈癌Ⅰ期与Ⅱa 期患者共134例不同临床分期宫颈鳞癌患者 SCC - Ag 水平和阳性率。术后将其分为两组(随访组和对照组),对照组只做常规宫颈病变筛查。随访两年观察比较两组患者术后两年内诊断出宫颈癌前病变的复发率。结果宫颈癌Ⅰ期和宫颈癌Ⅱa 期 SCC - Ag 水平、阳性率均明显高于宫颈上皮内瘤变(CINⅡ或 CINⅢ)组,差异具有统计学意义( P <0.05)。SCC - Ag 对术后检出宫颈肿瘤的复发率对比传统筛查方法,差异有显著性( P <0.05)。结论较高的血清 SCC 值可能意味着肿瘤恶性程度越高,血清 SCC - Ag 水平对于宫颈上皮内瘤变与宫颈癌早期的诊断、疗效判断、随访评估和复发后抢救性治疗措施均有重要的临床指导意义。%Objective To discuss the value of squamous cell carcinoma antigen in cervical precancerous lesions and early cervical cancer therapy. Methods Using ELISA for early detection,chemiluminescence review measurement of cervical intraepithelial neoplasia(CINⅡ or CINⅢ),patients with cervical cancer stage Ⅰ Ⅱa total 134 patients in different clinical stages of cervical squamous cell carcinoma SCC - Ag level and positive rate. They were divided into two groups after surgery,the control group only routine screening of cervical lesions,for a two - year fol-low - up. To observe and compare the recurrence rate of cervical lesions and early stage cervical cancer in two group of patients,more SCC - Ag value in the diagnosis of cervical cancer recurrence after surgery. Evaluating the value of SCC - Ag in the diagnosis of cervical cancer after surgical treatment of recurrence. Results The detection of postoperative recurrence rate of

  3. Are treatments for cervical precancerous lesions in less-developed countries safe enough to promote scaling-up of cervical screening programs? A systematic review

    Directory of Open Access Journals (Sweden)

    Stringer Jeffrey SA

    2010-04-01

    Full Text Available Abstract Background Since the mid-1990s, there have been growing efforts to prevent cervical cancer in less-developed countries through the development of innovative screening approaches such as visual inspection of the cervix associated with same day management of cervical lesions with cryotherapy or loop electrosurgical excision procedure (LEEP. In the past, promising cancer screening interventions have been widely promoted despite incomplete evidence, only to become the subject of intense controversies about ensuing net health benefit. Because the efficacy and effectiveness of the new protocols for global cervical cancer screening have not been well characterized yet, and as a contribution to the evaluation of the balance between the benefits and risks of these protocols, we reviewed the literature on the safety of cryotherapy and LEEP for cervical intraepithelial neoplasia (CIN in low- and middle-income countries. Methods We searched 12 databases (Medline, Google Scholar, Scopus, Cochrane Library, Web of Science, OCLC, PAIS International Database, WHO Global Health Library, CINAHL, Science.gov, NYAM Grey Literature Report, and POPLINE for original research published between January 1995 and April 2009. Both peer-reviewed publications and items of "grey" literature were retrieved; no language restriction was applied. We calculated the median (minimum, maximum reported rate for each harm considered. Because of limitations and heterogeneity in the data, no formal meta-analysis was performed. Results The search identified 32 articles that reported safety data from 24 cryotherapy and LEEP studies. The combined sample consisted of 6,902 women treated by cryotherapy and 4,524 women treated by LEEP. Most studies were conducted in reference or research settings in Asia and Africa. Short-term harms of cryotherapy and LEEP appeared to be similar to those described in the literature from high-income countries. Information was sparse on HIV-related harms

  4. ANALYSIS OF COLPOSCOPY EXAMINATION FOR THE CERVICAL CANER AND ITS PRECANCEROUS LESIONS%阴道镜检查在宫颈癌及癌前病变筛查中的分析

    Institute of Scientific and Technical Information of China (English)

    梅天琼; 李静

    2011-01-01

    [Objective] To analyze the effect of colposcopy examination for the cervical caner and its precancerous lesions. [Methods] From June 2009 to December 2011, 835 cases of cervical intraepithelial pathological change, were detected by colposcope and results were compared with biopsy pathology. [Results] The accordance rate of the colposcopy examination and pathology diagnosis diagnosed cervicitis, C1N I , CIN II , CIN HI and SCC were 59.7%, 62.7%, 74.0%, 63.9% and 81.8%. [Conclusion] Colposcopy examination in the diagnosis of cervical disease is more reliable, and with higher sensitivity for cervical cancer, which is valuable for examining cervical cancer and precancerous lesions.%[目的]分析阴道镜检查在宫颈癌及癌前病变筛查中的效果.[方法]2009年6月~2010年12月,将835例阴道镜检查异常者阴道镜检查结果与病理学检查诊断结果间进行对比分析.[结果]阴道镜检查为炎症、CINⅠ、CINⅡ、CINⅢ和SCC者与病理诊断结果比较,诊断符合率分别为59.7%、62.7%、74.0%、63.9%和81.8%.[结论]阴道镜下检查对宫颈疾病的诊断比较可靠,对宫颈癌的敏感性较高,对宫颈癌及癌前病变检查具有较高的临床价值.

  5. 宫颈病变与人乳头瘤病毒感染临床病例分析%Human papillomavirus testing in cervical cancer and precancerous lesions screening

    Institute of Scientific and Technical Information of China (English)

    陈艳; 王巧燕; 张小伟; 沈湘萍

    2015-01-01

    目的:评价人乳头瘤病毒( Human Papillomavirus,HPV)感染在宫颈病变早期筛查中的作用,为进一步诊断和判断预后提供依据。方法来我院进行宫颈癌筛查,因宫颈液基薄层细胞学检查存在异常细胞妇女413例,均进行阴道镜下组织活检和HPV-DNA分型。结果上皮内瘤样病变( CIN) I患者中以ASCUS检出符合率最高,CIN II患者中以LSIL检出符合率最高,CIN III患者中以HSIL检出符合率最高。慢性宫颈炎患者一种或多种高危型HPV (HR-HPV)感染率要低于CIN患者(χ2=32.105,P=0.000),宫颈癌HR-HPV感染率为100%;慢性宫颈炎患者与CIN患者低危型HPV(LR-HPV)感染率比较,差异无统计学意义(χ2=0.205,P=0.650)。宫颈病变患者以单一HR-HPV亚型感染更常见。结论宫颈癌及癌前病变与HPV感染密切相关,HR-HPV基因检测及分型在宫颈病变的预后判断、疗效监测等方面具有重要价值。%Objective To evaluate the role of HPV testing in early screening for cervical cancer and precancerous lesions so as to obtain scientific knowledge for diagnosis and prognosis of the disease.Method 413 women admitted to Dongyang People's Hospital for cervical cancer or precancerous lesions screening were subjected to cervical tissue biopsy under colposcopy and HPV DNA gene typing.Findings Atypical squamous cells ( ASCUS) with the highest rate were found in patients with cervical intraepithelial neoplasia ( CIN) I.Low squamous intraepithelial lesion ( LSIL) with the highest rate were found in patients with CIN II, and high squamous intraepithelial lesion ( HSIL) with the highest rate were found in patients with CIN III.One or more high risk HPV ( HR-HPV) types were detected in 32.6% of the chronic cervicitis patients compared to 63.0% of the CIN patients.HR-HPV infection rate was 100% in cervical cancer patients.The difference was statistically significant.Single HR

  6. Value of TCT Combined with Colposcopy in the Screening of Cervical Precancerous Lesions%TCT联合阴道镜在筛查宫颈癌前病变中的价值

    Institute of Scientific and Technical Information of China (English)

    黄瑞军; 李祟健

    2013-01-01

    [Objective] To explore the value of liquid-based cytology test(TCT) combined with colposcopy in the screening of cervical precancerous lesions. [Methods] Totally 982 patients with cervical precancerous lesion receiving TCT and colposcopy screening in clinic service of our gynecology department in our hospital from Jan. 2005 to Jan. 2012 were chosen. Multiple-site biopsy of cervixes of patients under colposcope was performed for pathological examination. The histopathology examination was used as the gold standard. The value of TCT and colposcopy in the diagnosis of cervical lesions was analyzed. [Results] Among the 982 females, 118 females were abnormal detected by using TCT. The specificity, sensitivity and missed diagnosis rate of TCT for the diagnosis of cervical intraepithelial neoplasia(CIN) were 96. 51%, 58. 55% and 1. 02%, respectively. The 163 females were abnormal detected by using colposcopy, and the specificity, sensitivity and missed diagnosis rate for CIN were 97. 47%, 93. 42% and 1. 02%, respectively. Furthermore, the specificity, the sensitivity and missed diagnosis rate of the combination of TCT and colposcopy for CIN were 98. 80% , 96. 03% and 0. 71% , respectively. [Conclusion] TCT combined with colposcopy can increase the detection rate of cervical precancerous lesion, and earlier screen out cervical precancerous lesions. Thus it is helpful for early diagnosis and treatment, and can improve the survival rate of patients.%[目的]探讨宫颈液基薄层细胞学(TCT)联合阴道镜在筛查宫颈癌前病变中的诊断价值.[方法]选择2005年1月至2012年1月在本院妇科门诊采用TCT和阴道镜检查筛查宫颈癌前病变的患者982例,同时在阴道镜下对患者宫颈进行多位点活检送病理检查.以组织病理学检查为金标准,分析TCT和阴道镜在筛查宫颈癌前病变中的诊断价值.[结果]在982例妇女中,TCT细胞学检查出异常者有118例,对诊断宫颈上皮内瘤样变(CIN)的特异性为96

  7. Human papillomavirus infections in Mexican women with normal cytology, precancerous lesions, and cervical cancer: type-specific prevalence and HPV coinfections.

    Science.gov (United States)

    Aguilar-Lemarroy, Adriana; Vallejo-Ruiz, Verónica; Cortés-Gutiérrez, Elva I; Salgado-Bernabé, Manuel Eduardo; Ramos-González, Norma Patricia; Ortega-Cervantes, Laura; Arias-Flores, Rafael; Medina-Díaz, Irma M; Hernández-Garza, Fernando; Santos-López, Gerardo; Piña-Sánchez, Patricia

    2015-05-01

    The prevalence and genotype distribution of human papillomavirus (HPV) provides the basis for designing HPV prevention programs. The prevalence rates of type-specific HPV and coinfections in samples of Mexican women were investigated in 822 women aged 18-87 years. HPV detection was performed using a Linear Array™ genotyping test. HPV infection was found in 12.4% of controls, 46.3% of those with cervical intraepithelial neoplasia 1, and 100% of those with cervical intraepithelial neoplasia 3 or cervical cancer. HPV 16 was the most prevalent type in all diagnosis groups. The HPV types most frequently found in cervical cancers were 16, 18, 45, 52, 58, and 39; HPV types 16, 62, 51, 84, 18, 53, and CP6108 were the most prevalent in control women. Considering HPV-positive samples only, coinfections occurred most often in controls (63%) and were less frequent in those with cervical cancer (26%). The most frequent viral types in coinfections with HPV 16 in control women were HPV 62, 51, and 84; in women with cervical cancers, HPV 18, 39, and 70 were most common. In conclusion, in addition to HPV types 16 and 18, types 45, 39, 58, 52, and 71 were found in cervical cancers in Mexican women (78%); among them, only 65% were attributable to HPV types 16 and 18. Therefore, it is necessary to consider these viral types in the design of new vaccines, and to determine whether certain HPV types coinfecting with HPV 16 in precursor lesions determine tumor progression or regression.

  8. Correlation of human papilloma virus presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry

    Directory of Open Access Journals (Sweden)

    Meenu Pujani

    2012-01-01

    Full Text Available Background : Cancer of cervix is the most common form of cancer in females of developing countries. Cervical cancer is the best example of common human malignancy with a proven infectious etiology. The data linking human papilloma virus (HPV infection with the epidemiology and pathogenesis of cervical neoplasia is convincing. There are various methods for detection of HPV like immunohistochemistry, polymerase chain reaction, liquid phase hybridization (hybrid capture test, in situ hybridization etc. Materials and Methods: We studied the profile of precancerous and cancerous lesions of uterine cervix and correlated human papilloma virus (HPV presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry. Total 50 cases were first studied, analyzed, and classified histologically and then immunohistochemistry was done. Results : Majority of the cases 36/50 (72% under study are squamous cell carcinomas, which is the most common carcinoma found in the uterine cervix. HPV positivity for all cases of cervical carcinoma was found to be 34.7% (16/46 cases, for carcinoma in situ, it was 50% (2/4 cases. It can be thus hypothesized that with decreasing differentiation, there is loss of expression of HPV in the cervical epithelial cells. Conclusion: To conclude, though this study confirms the correlation of HPV presence with precancerous and cancerous lesions of uterine cervix, it also suggests that there must be other co-factors involved in cervical carcinogenesis as well.

  9. Study on the relationship between high risk human papilloma virus infection and cervical cancer and precancerous lesions%高危人乳头瘤病毒感染与宫颈癌及癌前病变的关系探讨

    Institute of Scientific and Technical Information of China (English)

    余锦芬

    2012-01-01

    目的 探讨人乳头瘤病毒(HPV)感染与宫颈癌、癌前病变的关系及不同亚型HPV病毒重叠感染对宫颈癌、癌前病变的影响.方法 以148例宫颈癌及癌前病变患者为观察组并以96例宫颈炎患者为对照组,观察两组研究对象高危HPV病毒感染率、多重感染对宫颈癌、癌前病变的影响及不同病理类型宫颈癌高危HPV感染率.结果 HPV病毒感染率以鳞癌、腺癌居多;高危型HPV病毒感染率、双重感染率、多重感染率在宫颈癌中较高,CINⅢ期、CINⅡ期、CIN Ⅰ期依次降低,均高于宫颈炎中感染率.结论 高危型HPV病毒感染与宫颈癌的发生、发展密切相关,阻断高危型HPV病毒的持续感染可阻断癌前病变的进程,是防治宫颈癌的关键所在.%OBJECTIVE To explore the relationship between human papilloma virus (HPV) infection and cervical carcinoma, precancerous lesion and study the effects of different subtypes of HPV virus superinfection on cervical cancer, precancerous lesion. METHODS 148 cases of cervical cancer and precancerous lesions of patients in observation group, and 96 cases of cer-vicitis patients as control group, observed high risk HPV infection rate, the effect of multiple infection on cervical cancer and precancerous lesions, and high risk HPV infection rate of different pathological types of cervical cancer in subjects of two groups. RESULTS HPV virus infection rates were more in squamous cell carcinoma, adenocarcinoma; high-risk HPV infection rates, double infection rates, multiple infection rates was higher in cervical cancer, and in stage Ⅲ , CIN, CIN Ⅱ , CIN Ⅰ lowered in turn, they were higher than in the infection rate of cervicitis. CONCLUSION The high risk HPV virus infection is closely associated with occurrence and development of cervical cancer. Blocking high risk HPV virus persistent infection can block precancerous lesions of the process, it's the key to prevent and treat cervical cancer.

  10. HPV vaccination and allocative efficiency: regional analysis of the costs and benefits with the bivalent AS04-adjuvanted vaccine, from the perspective of public health, for the prevention of cervical cancer and its pre-cancerous lesions

    Directory of Open Access Journals (Sweden)

    Paolo Bonanni

    2012-11-01

    Full Text Available Introduction: by means of the decisions on whether to introduce the HPV vaccination, Public Health has already established the importance of associating the vaccination strategy to the policy of secondary prevention. The screening + vaccination strategy is more effective than the two methods taken individually. In support of this combined strategy and in order to make available per each region concrete elements for their regional planning, an assessment has been made, which also takes into account the effect of cross-protection regarding high-risk strains not contained in both vaccines, bivalent and quadrivalent, and more frequently responsible for pre-cancerous lesions and cervical cancer (CCU. This analysis evaluates the costs and benefits of screening + vaccination strategy in a 12-year-old female cohort. Furthermore, the paper provides results that may be useful to assess the opportunity to extend the vaccination to a second cohort of 24-25-year-old women. The analysis is preceded by a brief summary of CCU epidemiology available data, public health policies that give precise guidelines for vaccination strategies and analytical tools suitable to support public policy makers to efficiently allocate resources. Methods: two different models were used for two regional analyses.The vaccines may have different sustained- and cross-protection levels against non-vaccine oncogenic HPV-types. In the first analysis, a prevalence-based model estimated the potential net difference in HPV-related lesions (abnormal pap smear, cervical intraepithelial neoplasia (CIN, cervical cancer (CC and genital warts (GW and associated costs generated by the two vaccines. Vaccine efficacy rates were based on published data for each vaccine. Lifetime vaccine efficacy was assumed. Results are reported over one year after reaching a steady state. Incidence and treatment costs were obtained from Italian and European sources. We also performed a cost-effectiveness analysis

  11. Association between selected oral pathogens and gastric precancerous lesions.

    Directory of Open Access Journals (Sweden)

    Christian R Salazar

    Full Text Available We examined whether colonization of selected oral pathogens is associated with gastric precancerous lesions in a cross-sectional study. A total of 119 participants were included, of which 37 were cases of chronic atrophic gastritis, intestinal metaplasia, or dysplasia. An oral examination was performed to measure periodontal indices. Plaque and saliva samples were tested with real-time quantitative PCR for DNA levels of pathogens related to periodontal disease (Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, Actinobacillus actinomycetemcomitans and dental caries (Streptococcus mutans and S. sobrinus. There were no consistent associations between DNA levels of selected bacterial species and gastric precancerous lesions, although an elevated but non-significant odds ratio (OR for gastric precancerous lesions was observed in relation to increasing colonization of A. actinomycetemcomitans (OR = 1.36 for one standard deviation increase, 95% Confidence Interval = 0.87-2.12, P. gingivalis (OR = 1.12, 0.67-1.88 and T. denticola (OR = 1.34, 0.83-2.12 measured in plaque. To assess the influence of specific long-term infection, stratified analyses by levels of periodontal indices were conducted. A. actinomycetemcomitans was significantly associated with gastric precancerous lesions (OR = 2.51, 1.13-5.56 among those with ≥ median of percent tooth sites with PD ≥ 3 mm, compared with no association among those below the median (OR = 0.86, 0.43-1.72. A significantly stronger relationship was observed between the cumulative bacterial burden score of periodontal disease-related pathogens and gastric precancerous lesions among those with higher versus lower levels of periodontal disease indices (p-values for interactions: 0.03-0.06. Among individuals with periodontal disease, high levels of colonization of periodontal pathogens are associated with an increased risk of gastric precancerous lesions.

  12. 检测尿中巯基筛诊宫颈癌与癌前病变的临床意义%Clinical Significance of Detection of Sulfhydryl in Urine for Screening Cer-vical Precancerous Lesion and Cancer

    Institute of Scientific and Technical Information of China (English)

    刘爽

    2015-01-01

    Objective To study the clinical significance of detection of sulfhydryl in urine for screening cervical precancerous le-sion and cancer. Methods 90 cases with cervical disease visited our hospital and underwent physical examination from January 2014 to December 2014 were divided into three groups, the observation group, control group 1 and control group 2 with 30 cases in each in accordance with the stochastic method. The observation group underwent the testing of sulfhydryl in urine, control group 1 underwent cervical liquid based cytology(TCT), and control group 2 underwent high-risk human papilloma virus (HPV) test. And the significance of detection was compared between the three groups. Results The detection rate of cervical precancerous lesion and cancer and detection sensitivity in the observation group were basically corresponded to the final pathology results with no sta-tistically significant difference(P>0.05). Compared with the final pathology results, the detection sensitivity was much lower in con-trol group 1 and control group 2, and there was statistically significant difference in the detection rate of cervical precancerous le-sion and cancer of control group 1 and control group 2 with that of final pathology results (P<0.05); the level of patient satisfaction was 96.67%in the observation group, 83.33%in control group1, and 80.00% in control group 2, the observation group had much higher level of patient satisfaction (P<0.05). Conclusion Compared with TCT and high-risk HPV test, the clinical value of testing of sulfhydryl in urine for screening cervical precancerous lesion and cancer is greater, which can significantly improve the detec-tion rate and sensitivity, increase the level of patient satisfaction, so it is worthy of clinical promotion and application.%目的:研究检测尿中巯基筛诊宫颈癌与癌前病变的临床意义。方法按照随机分号法将在该院2014年1-12月间就诊及体检的90例宫颈疾病患者均分为3

  13. Expression and Significance of RhoC and ROCKⅠ in Precancerous Lesions of Uterine Cervix and Cervical Cancer%RhoC、ROCKⅠ在宫颈癌前病变和宫颈癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    陈嵘; 耿力; 杨京京; 张幼怡; 李子健

    2011-01-01

    Objective : To explore the expressions of RhoC and ROCK Ⅰ in cervical intraepithelial neoplasia ( CIN ) and cervical cancer as well as their relationship with the genesis of cervical cancer. Methods : The expressions of RhoC and ROCK Ⅰ in cervical cancers( n = 27 ) , CIN Ⅲ( n = 28 ) , CIN Ⅱ ( n = 28 ) , CIN Ⅰ ( n = 25 )and chronic inflammations( n = 28 )were detected by immunohistochemistry. Results : The expressions of RhoC and ROCK Ⅰ in CIN Ⅱ ,CIN Ⅲand cervical cancer were significantly higher than that of CIN Ⅰ and chronic inflammation ( P < 0. 01 ). The severer the cervical lesion was, the higher expression level of Rhoc and ROCK Ⅰ .And the expression level of RhoC was of positive correlation with that of ROCK Ⅰ in CIN Ⅱ and above stages( rs = 0. 605 . P < 0. 001 ).Conclusion: RhoC/ROCK Ⅰ pathway may play an important role in the progression of prec:ancerous lesions of uterine cervix and the genesis of cervical cancer when RhoC expression is at a high level. RhoC/ROCK Ⅰ would be a new target of clinical therapy. RhoC expression may be a good marker for improving treatment scheme and evaluating the risk of canceration.%目的:探讨RhoC和ROCKⅠ基因在不同等级宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)和宫颈癌中的表达情况及二者与宫颈癌发生的相关性.方法:采用免疫组化的方法检测慢性炎症28例、CINⅠ25例、CINⅡ28例、CINⅢ28例、宫颈癌27例中RhoC和ROCKⅠ基因的表达情况.结果:RhoC和ROCKⅠ在CINⅡ、CINⅢ和宫颈癌中的表达水平显著高于CINⅠ和慢性炎症,病变程度越重,表达水平越高,差异有统计学意义(P<0.01).RhoC和ROCKⅠ的表达在CINⅡ及以上病变中成正相关(rs=0.605,P<0.001).结论:RhoC在表达水平较高时可能通过ROCKⅠ促进宫颈癌前病变的进展和宫颈癌的发生,RhoC/ROCKⅠ可能成为早期治疗的新靶点.宫颈组织中RhoC的表达水平有助于完善宫颈病变患者的治

  14. 血清SccAg、CA125、CA19-9在宫颈癌及癌前病变中的表达及意义%Expressions and significances of serum SccAg, CA125, and CA19 -9 in cervical cancer and cervical precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    甘玉杰; 熊小英; 符丽华; 谭玲玲

    2012-01-01

    Objective: To study the expressions and significances of serum squamous cell carcinoma antigen (SccAg) , cancer antigen 125 (CA125) , carbohydrate antigen 19-9 (CA19 -9) in cervical cancer and cervical precancerous lesion. Methods; Thirty-four serum samples in normal control group, one hundred and twenty - six serum samples in cervical intraepithelial neoplasia (CIN) group, and fifty - seven serum samples in cervical cancer group were detected, the three tumor markers in cervical cancer of different clinical stages, differentiation degrees, and pathological types were compared. Results; Serum SccAg expression changed in cervical precancerous lesion, the changes of CA125 and CA19 - 9 were not obvious. There was a positive correlation between serum SccAg level and clinical stages, pathological degrees, and lymph node metastasis of cervical cancer, CA125 and CA19 -9 had great application values in detection of cervical adeno-carcinoma. Conclusion; Comprehensively analyzing tumor markers can provide guidance and data for assisted diagnosis, diagnosis, treatment, and prognosis of the patients with cervical cancer.%目的:研究鳞状上皮细胞癌抗原(SccAg)、癌抗原125 (CA125)、癌抗原19-9 (CA19 -9)在癌前病变及宫颈癌中的表达和意义.方法:检测正常对照组血清34例、宫颈上皮内瘤变(CIN)组126例、宫颈癌组57例,对不同临床分期、分化程度和病理类型宫颈癌的三种肿瘤标志物进行比较.结果:SccAg在宫颈癌前病变已有变化,CA125和CA19 -9变化不明显,血清中SccAg的水平与肿瘤临床分期、肿瘤病理分级、淋巴结转移呈正相关,CA125和CA19 -9在检测腺癌中更有应用价值.结论:综合分析宫颈癌患者的肿瘤标志物能为患者的辅助诊断、病情诊治、预后提供指导信息.

  15. 阴道镜联合宫颈锥切对宫颈癌及其癌前病变患者的诊断价值分析%Diagnostic Value of Colposcopy and Cervical Conization for Cervical Cancer and Precancerous Lesion

    Institute of Scientific and Technical Information of China (English)

    易黎; 归倩

    2014-01-01

    Objective To explore the diagnostic value of colposcopy and cervical conization for cervical cancer and pre -cancerous lesion .Methods 618 cases with cervical cancer and precancerous lesion were selected as research subjects ,including 32 cases of CINⅠ,58 cases of CINⅡ,40 cases of CIN Ⅲ and 8 cases of cervical cancer .Results The sensitivity,specificity, positive predictive value and negative predictive value of colposcopy for cervical cancer and precancerous lesion were 74.6%, 93.8%,77.4%and 92.8%,respectively;The rates of correct diagnosis of colposcopy for CIN Ⅰ,CIN Ⅱ and CIN Ⅲ were 59.4%(19 cases),79.3%(46 cases) and 82.5%(33 cases);22 cases of CINⅠ,50 cases of CINⅡand 36 cases of CIN Ⅲwere detected by cervical conization;5 cases (62.5%) and 2 cases(25.0%) of cervical cancer were detected by colposcopy or cervical conization respectively .Combined diagnostic rate of colposcopy and cervical conization was 87.5%.Conclusion Colpos-copy and cervical conization improve the diagnostic rate of cervical cancer and precancerous lesion .%目的:探讨阴道镜联合宫颈锥切( LEEP)对宫颈癌及其癌前病变患者的诊断价值。方法选择618例宫颈癌及癌前病变患者为研究对象,其中病理诊断确诊宫颈上皮内瘤变( CIN)Ⅰ级32例,Ⅱ级58例,Ⅲ级40例,宫颈浸润癌8例。采用宫颈锥切治疗,分析手术前后病理诊断情况。结果在618例患者中,阴道镜诊断宫颈癌及癌前病变的灵敏度、特异度、阳性预测值及阴性预测值分别为74.6%、93.8%、77.4%和92.8%;阴道镜正确诊断CINⅠ级、CINⅡ级及CINⅢ级分别为19例(59.4%)、46例(79.3%)及33例(82.5%);子宫锥切术检出CINⅠ级22例,Ⅱ级50例,Ⅲ级36例;阴道镜诊断宫颈浸润癌5例(62.5%),经宫颈锥切另发现2例(25.0%),两者联合诊断宫颈浸润癌准确率为87.5%。结论阴道镜联合宫颈锥切可

  16. Association between oral health and gastric precancerous lesions

    OpenAIRE

    Salazar, Christian R.; Francois, Fritz; Li, Yihong; Corby, Patricia; Hays, Rosemary; Leung, Celine; Bedi, Sukhleen; Segers, Stephanie; Queiroz, Erica; SUN, JINGHUA; Wang, Beverly; Ho, Hao; Craig, Ronald; Cruz, Gustavo D.; Blaser, Martin J.

    2011-01-01

    Although recent studies have suggested that tooth loss is positively related to the risk of gastric non-cardia cancer, the underlying oral health conditions potentially responsible for the association remain unknown. We investigated whether clinical and behavioral measures of oral health are associated with the risk of gastric precancerous lesions. We conducted a cross-sectional study of 131 patients undergoing upper gastrointestinal endoscopy. Cases were defined as those with gastric precanc...

  17. 高危型人乳头瘤病毒感染与宫颈癌前病变及宫颈癌的相关性分析%Correlation analysis between high-risk human papillomavirus infection and precancerous lesions of cervix uteri and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    李曼红; 曾庆纯

    2015-01-01

    Objective To investigate the correlation between high-risk human papillomavirus (HPV) infection, precancerous lesions of cervix uteri and cervical cancer. Methods Among 120 patients with cervical cancer or precancerous lesions, there were 60 cases with precancerous lesions of cervix uteri (precancerous lesions group) and 60 cases with cervical cancer (cervical cancer group). They were chosen as the observation group, and another 60 patients without cytological changes of cervix uteri in colpitis were taken as the control group. Cervix uteri thinprep cytology test, cervical scraping smear, and immunohistochemcial method were applied for screening and analysis, and Ann PCR-reverse dot blot method was used for detection. Examination results were compared between the groups. Results The total positive rate of high-risk HPV was 56.67%, and the positive rates of high-risk HPV in precancerous lesions group, cervical cancer group and control group were respectively 65%, 95%, and 10%. The difference of positive rate between the three groups had statistical significance (P<0.05). Conclusion High-risk HPV has close relationship with precancerous lesions of cervix uteri and cervical cancer, and the infection of high-risk HPV was mainly in HPV16.%目的:探讨高危型人乳头瘤病毒(HPV)感染与宫颈癌前病变以及宫颈癌的相关性。方法120例宫颈癌或是癌前病变患者,其中60例为宫颈癌前病变患者(癌前病变组),60例为宫颈癌患者(宫颈癌组),将其归为观察组,同时期60例阴道炎宫颈无细胞学改变的患者设为对照组。采用宫颈液基细胞学检查法与宫颈刮片、免疫组化予以筛查分析,同时还采用达安PCR-反向点杂交法检测,对比分析各组检测结果。结果高危型HPV总阳性率为56.67%,癌前病变组、宫颈癌组以及对照组患者的高危型HPV阳性率分别为65%、95%、10%,三组患者的阳性率对比差异具有统计学意义(P<0.05)。结论高

  18. 宫颈液基细胞学检查与高危型HPV检测早期筛查宫颈癌前病变的对比分析%Comparative study of liquid-based cervical cytology and high-risk HPV testing on ear-ly screening for cervical precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    刘志红; 邹艳芬

    2016-01-01

    Objective:To study the effect of cervical cytology liquid-based and high-risk HPV screening for early detection of cervical lesions before. Methods:From March 2013 to March 2014 to select cervical screening 700 cases as subjects. Using high-risk HPV testing and liquid-based cervical cytology screening to test early cervical lesions. Results:In 700 cases with high-risk HPV-positive in 47 cases. HPV16 type was the most,accounting for 48. 94 percent,followed by HPV18,accounting for 21. 28%. Liquid-based cytology in cervical smear abnormalities in patients with 40 cases. LSIL accounted for 2. 71%,HSIL accounted for 0. 57%. TCT method combined high-risk HPV,LSIL and HSIL were 2. 32% and 0. 54%,and TCT was 0. 45% and 0. 19%. Conclusion:High-risk HPV tes-ting can improve the accuracy of cervical cancer screening,combined with liquid-based cervical cytology high-risk HPV testing can improve the success rate of screening for cervical precancerous lesions.%目的:对比研究宫颈液基细胞学检查与高危型HPV检测早期筛查宫颈癌前病变.方法:选取2013年3月到2014年3月接受宫颈病理变化筛选检查的700例患者作为研究对象.采用高危型HPV检查和宫颈液基细胞检查进行早期宫颈癌前病变筛查.结果:在700例患者中,高危型HPV检查结果阳性有47例.其中HPV16型最多,占48.94%,其次是HPV18型,占21.28%.在宫颈液基细胞学检查中涂片异常病人有40例.其中LSIL占2.71%,HSIL占0.57%.高危HPV联合TCT方法,LSIL和HSIL分别是2.32%和0.54%;而TCT则是0.45%和0.19%.结论:高危型HPV检查能够提高宫颈癌前筛查的准确率,宫颈液基细胞检查联合高危HPV检查能够提高宫颈癌前病变筛查成功率.

  19. Awareness of patients about existing oral precancerous lesions/conditions in Nashik city of Maharashtra

    Directory of Open Access Journals (Sweden)

    Bhushan Sukdeo Ahire

    2016-01-01

    Full Text Available Introduction: Many oral squamous cell carcinomas develop from premalignant lesions/conditions of oral cavity. Hence, the awareness of such lesions/conditions is important. Aim: To assess the awareness about existing oral precancerous lesions/conditions among patients arriving for dental treatment at a dental hospital, in Nashik city of Maharashtra. Materials and Methods: A questionnaire was used to collect information from 80 patients with existing oral precancerous lesions/conditions attending the dental hospital, in Nashik city of Maharashtra. The questionnaire included questions to ascertain information on sociodemographic parameters, awareness, and sources of information about of oral precancerous lesions/conditions, habit of tobacco, areca nut chewing, smoking, alcohol, and combined habits. Results: We found that 40% (n = 32 respondents knew about the existence of lesion in their mouth of which only 50% (out of 40% had thought that it was precancerous lesion/condition. Among all subjects, only 47.5% (n = 38 were aware of oral precancerous lesions/conditions. Television was the major source of information about oral precancerous lesions/conditions almost all the subjects (97.5% wanted more information about oral precancerous lesions/conditions but through television (42.5% and lectures (27.5%. Conclusion: Awareness of patients (coming to hospital about oral precancerous lesions/conditions was found to be low. The people must be made aware of symptoms, signs, and preventive strategies of oral precancerous lesions/conditions through their preferred media – television and lectures.

  20. Evaluation of computer-assisted ThinPrep imaging system in the screening of cervical cancer and precancerous lesion%TIS在宫颈癌和癌前病变筛查中的价值评价

    Institute of Scientific and Technical Information of China (English)

    米贤军; 王莹; 沈铿; 吴秋良; 肖琳; 陈昂; 徐秀梅; 孪峰; 钟守军

    2013-01-01

    目的 评价新柏氏电脑辅助阅片系统(TIS)对宫颈癌及癌前病变的诊断价值.方法 收集2011年6月至2012年6月参加中山市大规模免费宫颈癌筛查中妇女的资料,选择做过TIS检测并追踪到有确切组织病理学诊断结果的600例病例为研究对象,将ASCUS/AGC+病变列为细胞学阳性病例,将CIN1+病变列为病理学阳性病例,以病理学检查为金标准,评价TIS检测的诊断价值.结果 600例妇女中,TIS检测结果为癌(CA)9例,高度鳞状上皮内病变(HSIL) 66例,低度鳞状上皮内病变(LSIL) 104例,不除外高度鳞状上皮内病变的不典型鳞状细胞(ASC-H) 21例,非典型鳞状细胞/非典型腺细胞(ASCUS/AGC) 30例,正常(WNL) 370例.以病理学诊断结果为金标准,TIS检测的灵敏度为93.95%,特异度为92.73%,约登指数为0.867,阳性似然比12.92,阴性似然比0.065,Kappa值0.854,总符合率为93.17%,阳性预测值87.83%,阴性预测值96.49%.TIS与病理学结果的符合率在CA、HSIL、LSIL中分别为100%、90.91%和72.12%,TIS对CA和HSIL的符合率高于LSIL (P<0.01).结论 TIS对宫颈癌及癌前病变有较高的诊断价值,特别是对CA和HSIL.它是一种高效、高质量和高准确率的宫颈癌及癌前病变筛查技术.%Objective To study the diagnostic value of computer-assisted ThinPrep imaging system(TIS) in the screening of cervical cancer and precancerous lesion.Methods The clinical data of 600 cases who participated voluntarily in the screening of large-scale cervical cancer for free in Zhongshan were collected from June 2011 to June 2012.ASCUS/AGC and above were postive cytology and the postive results of pathological examination were CIN and above.Histopathological results were used as gold standard to calculate the diagnostic value of TIS technique.Results A total of 600 cases were detected by TIS,and the testing results showed 9 cases of CA,66 cases of HSIL,104 cases of LSIL,21 cases of ASC-H,30 cases of ASCUS

  1. Expression of ornithine decarboxylase in precancerous and cancerous gastric lesions

    Institute of Scientific and Technical Information of China (English)

    Xin-Pu Miao; Jian-Sheng Li; Hui-Yan Li; Shi-Ping Zeng; Ye Zhao; Jiang-Zheng Zeng

    2007-01-01

    AIM:To investigate the expression of ornithine decarboxylase (ODC) in precancerous and cancerous gastric lesions.METHODS: We studied the expression of ODC in gastric mucosa from patients with chronic superficial gastritis (CSG, n = 32), chronic atrophic gastritis [CAG, n = 43;15 with and 28 without intestinal metaplasia (IM)],gastric dysplasia (DYS, n = 11) and gastric cancer (GC,n = 48) tissues using immunohistochemical staining. All 134 biopsy specimens of gastric mucosa were collected by gastroscopy.METHODS: The positive rate of ODC expression was 34.4%, 42.9%, 73.3%, 81.8% and 91.7% in cases with CSG, CAG without IM, CAG with IM, DYS and GC, respectively (P < 0.01), The positive rate of ODC expression increased in the order of CSG < CAG (without IM) < CAG (with IM) < DYS and finally, GC. In addition,ODC positive immunostaining rate was lower in welldifferentiated GC than in poorly-differentiated GC (P <0.05).CONCLUSION: The expression of ODC is positively correlated with the degree of malignity of gastric mucosa and development of gastric lesions. This finding indicates that ODC may be used as a good biomarker in the screening and diagnosis of precancerous lesions.

  2. Effect of jianpiyiwei capsule on gastric precancerous lesions in rats

    Institute of Scientific and Technical Information of China (English)

    Xue-Ying Shi; Feng-Zhi Zhao; Xin Dai; Lian-Sheng Ma; Xiu-Yu Dong; Jie Fang

    2002-01-01

    AIM:To evaluate the therapeutic effect of compoundChinese drugs, Jianpiyiwei capsule (JPYW) on gastricprecancerous lesions in rats and to explore itsmechanism of action.METHODS:Model of gastric precancerous lesions wasconstructed in male Wistar rats: a metal spring wasinserted and fixed through pyloric sphincter. One weekafter recovery, each rat was given 50-60 ℃ hot pastecontainingt50 g/L NaCl 2 mL orally, twice a week for15 weeks.Then 10 normal and 11 model rats wereanaesthetized, after the measurement of gastricmucosa blood flow (GMBF), the rats were killed andthe mucosal hexosamines and malonic dialdehyde(MDA) were measured. The morphological changes ofgastric mucosa were observed macroscopically andmicroscopically, and by an automatic imaging analysissystem. Other rats were treated with JPYW 1.5 g/kg.d-1or 4.5 g/kg@d-1, or distilled water as negative controlrespectively (n=-10 in each group). After 12 weeks, allthe rats were examined as above.RESULTS: The gastric mucosa of model rats showedchronic atrophic gastritis with dysplasia and intestinalmetaplasia (IM), GMBF and hexosamine content werereduced significantly and MDA was increased ascompared to the normal group (P<0.01). After 12 weekstreatment, the pathological changes of the negativecontrol group became worsened, while in JPYW treatedgroups the changes were modified with significantincrease of GMBF and reduction of MDA, although thehexosamine concentration increased only mildly.CONCLUSION: JPYW increases GMBF and reduces MDAcontent in gastric mucosa and has therapeutic effectson gastric precancerous lesions.

  3. 叶酸与DNA甲基转移酶1在宫颈癌及癌前病变中的作用%Effect of folic acid and DNA methyltransferase 1 on cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    王金桃; 霍晓旭; 丁玲; 郝敏; 樊金荣; 张丽; 赵维敏

    2011-01-01

    Objective To explore the effect of folic acid and DNA methyltransferase 1 (DNMT1) on cervical cancer and cervix precancerous lesion. Methods 100 patients with cervix squamouscell carcinoma (SCC), 101 patients with cervical intraepithelial neoplasm (CIN) and 109 patients with cervix inflammation (CI) diagnosed by histology were included in this study. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and Western blot were used to detect the levels of serum folate, HPV16 infection and the expression of DNMT1 protein,respectively. Results The average levels of serum folate were (2.60 ± 1.61) ng/ml, (3.14 + 2.08) ng/ml and (3.32+1.74) ng/ml,and the expression of DNMT1 protein were 2.40 + 0.99,1.88 + 0.33 and 0.89 ± 0.29 in the group of SCC, CIN and CI, respectively.The relationship of folate levels and DNMT1 protein expression showed inverse correlation (r=-0.186, P=0.00l). The results in our study indicated that there was an additive interaction between low-level of serum folate and high-expressionof DNMT1 protein related to the risk of CIN and SCC, with OR value as 2.50(95%C/: 1.21-9.22) and 6.03 (95%C/: 2.79-21.72) respectively. The relative excessrisk of interaction (RERI) , attributableproportion of interaction (API) and synergy index (S) were 0.92, 0.36 and 2.59 in the CIN group while 2.47, 0.41 and 1.96 in the SCC group. Conclusion The low level of serum folate and high expression of DNMT1 protein seemed to be associated with high risk of cervical cancer and its precancerous lesion. It suggested that there might be a synergistic action between serum folate and DNMT1 in the progression of cervix carcinogenesis.%目的 探讨叶酸和DNA甲基转移酶1(DNMT1)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者100例、宫颈上皮内瘤样变(CrN)患者101例和宫颈炎(CI)患者109例为研究对象.在收集全部对象人口学特征、生殖因素等相关资料

  4. Role of Visual Inspection of Cervix with Acetic Acid (VIA in Detecting Precancerous Lesions of Cervix

    Directory of Open Access Journals (Sweden)

    Kamrun Nessa

    2014-01-01

    Full Text Available Background: Carcinoma of cervix is the most common malignancy in female and a major public health problem worldwide. It is the leading cause of death from cancer among women in low resource settings. In Bangladesh, mortality rate is high as most of the cases with cervical cancer are diagnosed in advanced stage. World Health Organization considers cervical cancer as a preventable disease as it can be identified in preinvasive stage. Considerable efforts have been given in detection and treatment of the condition all over the world. A number of cervical cancer screening tests are available. Among them, visual inspection of cervix with acetic acid is rational and can be competently performed by physicians with proper training. Objective: To find out the feasibility of the visual inspection of cervix with acetic acid for the detection of the precancerous lesions of the cervix in our country. Materials and Methods: This cross sectional, analytical study was carried out among the patients attending the outpatient department of Bangabandhu Sheikh Mujib Medical University (BSMMU who were VIA positive and sent for colposcopy in the colposcopy clinic in the department of Obstetrics and Gynecology in BSMMU from June to December 2004. Two hundred samples were considered for this study. Results: Out of 200 cases, colposcopically 85% had CIN and invasive lesions, 4% had inflammatory lesions while 11% had normal findings. Colposcopy directed punch biopsy revealed positive lesions in 81%, 4% had inflammatory lesions while 15% had normal findings. Conclusion: The study concluded that VIA and colposcopy are the important methods in the evaluation of cervical premalignancy. VIA may be an important tool for screening of cervical cancer in low resource settings as it is simple, easy to perform and cost-effective. After screening, VIA positive cases must be referred for colposcopic evaluation. We can screen cervical cancer by VIA all over the country and thus reduce

  5. 阴道镜联合液基薄层细胞学检测对宫颈癌前病变的诊断价值%Diagnostic value of colposcopy combined new thinprep pap test in cervical cytology precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    王继红

    2011-01-01

    目的 探讨阴道镜联合液基薄层细胞学检测(TCT)在宫颈病变筛查中的诊断价值.方法 选取行宫颈细胞学检查患者1 266例,行阴道镜和液基薄层细胞学联合检查.结果 本组所检1 266例中异常者169例,宫颈病变检出率13.35%,异常者中宫颈上皮内病变及鳞癌共126例,占9.99%.宫颈上皮内病变发病率检测各年龄组之间差异无统计学意义(P>0.05).结论 阴道镜联合液基薄层细胞学检测是宫颈癌前病变可靠的早期临床诊断方法.%Objective To evaluate the new colposcopy combined cytology thinprep pap test(TCT) in cervical screening in the diagnosis of disease. Methods 1 266 patients detected by gynecologic cervical cytology were conducted on colposcopy and liquid-based thin-layer cytology of the Joint Inspection. Results 1 266 cases in which seized 169 cases of abnormal cervical lesion detection rate of 13. 35%,abnormalities in cervical squamous intraepithelial lesions and 126 cases,accounting for 9.99%. The incidence of cervical intraepithelial lesions detected no difference between age groups was statistically significant(P>0.05). Conclusion Thinprep cytology combined colposcopy cervical precancerous lesions was a reliable method for early diagnosis.

  6. Liquid Based Cytology and Colposcopy in the Screening of Cervical Cancer and Precancerous Lesions%宫颈液基细胞学及阴道镜在宫颈癌及癌前病变中的筛查作用

    Institute of Scientific and Technical Information of China (English)

    王清; 胡霞

    2015-01-01

    目的:分析液基细胞学和阴道镜在宫颈癌及癌前病变中的筛查作用,评估液基细胞学和阴道镜在宫颈癌及癌前病变筛查中的临床应用价值。方法回顾性分析2013年5月-2014年8月接受宫颈癌筛查的500例女性的临床资料,所有体检者都接受阴道镜检查和液基细胞学检查。结果阴道镜检测假阳性患者有11例,阴道镜检测的特异性为97.39%(412/423),漏诊率为1.00%(5/500);液基细胞学诊断的假阳性患者有15例,由检测结果可知液基细胞学检测宫颈癌的检测特异性为96.45%(408/423),漏诊率为6.40%(32/500)。结论液基细胞学和阴道镜在宫颈癌及癌前病变筛查中具有临床应用价值,阴道镜对宫颈癌及癌前病变的检出率较液基细胞学高。%Objective To study the application value of liquid based cytology and colposcopy in the screening of cervical cancer and precancerous lesions .Methods Retrospective analysis of clinical data of 500 female patients who underwent cervical cancer screening were conducted .All subjects received colposcopy and liquid based cytology .Results Colposcopy results showed that false positive were 11 cases,the specificity of colposcopy was 97.39%(412 /423),the rate of missed diagnosis was 1.00%(5 /500);liquid based cytology diagnosis showed that false positive were 15 cases,specificity was 96.45%(408/423),the rate of missed diagnosis was 6.40%(32 /500).Conclusion Liquid based cytology and colposcopy has clinical application value in the screening of cervical cancer and precancerous lesions ,Colposcopy for cervical cancer and precancerous lesions has higher detec -tion rate compared with liquid based cytology .

  7. Clinical Significance of HC2-HPV Assay Combined with Liquid Based Cytology in the Screen of Cervical Cancer and Precancerous Lesions%HC2-HPV 检测法联合液基细胞学在宫颈癌及癌前病变筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    徐艺; 杨静秀; 王玉平

    2016-01-01

    目的:探讨HC2-HPV检测法联合液基细胞学在筛查宫颈癌及癌前病变中的意义。方法应用HC2-HPV检测法联合液基细胞学技术对体检的患者进行免费宫颈癌及癌前病变的筛查。对不典型增生患者或(和) HC2-HPV检测阳性的患者行阴道镜下宫颈活检。结果液基细胞学检查对宫颈高度病变的检出率为11.0%(11/100);HC2-HPV检测对宫颈高度病变的检出率为27.2%(22/81),对宫颈癌的检出率高达100.0%。 HC2-HPV检测法联合液基细胞学检测阳性与病理检查阳性符合率为64.2%(52/81),显著优于TCT(52.0%)单独检查时的阳性符合率(P<0.05,χ2=11.39)。结论 HC2-HPV检测法联合液基细胞学检测对宫颈癌及癌前病变的筛查具有重要的临床推广价值。%Objective To investigate the significance of HC 2-HPV assay combined with liquid based cytology in the screen of cervical cancer and precancerous lesions .Methods HC2-HPV assay combined with liquid based cytology was used in the screen of cervical cancer and precancerous lesions .Cervical biopsy was performed in patients with atypical hyperplasia or (and) HC2-HPV positive patients.Results Detection rate of liquid based cytology for cervical lesion was 11.0%(11/100),and detection rates of HC2-HPV for cervical lesion and cervical cancer were 27.2%(22/81) and 100.0%.The coincidence rate of HC2-HPV combined with liquid based cytology and biopsy results was 64.2%(52/81),which was significantly higher than that of TCT(52.0%)(P<0.05,χ2 =11.39).Conclusion HC2-HPV assay combined with liquid based cytology test has important clinical value in the screen of cervical cancer and precancerous lesions .

  8. Lugol's iodine identifies dysplastic tissue in precancerous lesions: A clinical trial

    Science.gov (United States)

    Fatima, Shereen; Basu, Rajarshi; Hallur, Neelakamal H.

    2016-01-01

    Introduction: Intraepithelial dysplasia, or “invisible” precancerous lesions, provides a challenge for visualization to the surgical team. The prognostic relevance of dysplasia and carcinoma in situ at surgical margins is well documented. Materials and Methods: We evaluated the use of Lugol's iodine in visualizing the surgical margins of dysplastic tissue by an observational study of 100 patients having oral precancerous lesions between June 2013 and March 2016. Conclusion: Lugol's iodine is a simple, inexpensive, and apparently effective means of diagnosing and visualizing the surgical margins of the dysplastic tissue in oral precancerous lesions. PMID:28299253

  9. Diagnostic validity of cervical precancerous lesions by liquid-based cytology technique combined with high-risk HPV-DNA detection%液基细胞学技术联合高危HPV-DNA检测对宫颈癌前病变的诊断效度

    Institute of Scientific and Technical Information of China (English)

    王泽曼; 陈玉; 郑建鹏

    2011-01-01

    目的:探讨液基细胞学检查技术(LCT)联合高危人乳头状瘤病毒DNA(HPV-DNA)检测诊断宫颈癌前病变的效度.方法:对2007年12月~2010年12月来我院行体检的19~65岁的6 521名女性采用LCT进行宫颈癌的筛查,以及HPV分型基因芯片检测系统进行18种高危HPV基因亚型检测.对上述检测阳性者行阴道镜下宫颈活组织检查,对检测均阴性者依其意愿进行阴道镜下宫颈活组织检查.结果:LCT阳性(≥ASCUS)152例,HPV阳性86例,其中二者均为阳性的有42例,LCT和HPV均为阴性的有6 325例;LCT阳性的152例和HPV阳性的86例中病理活组织检查结果为阳性(≥CIN I)的分别有112例和68例,其中LCT和HPV均阳性的42例中病理活组织检查阳性的有34例.LCT和HPV均为阴性的6 325例中有2 000人自愿行病理检查,其中1人病理检查结果为阳性.LCT诊断宫颈癌前病变的灵敏度为76.19%,特异度为98.05%;HPV检测诊断宫颈癌前病变的灵敏度为46.26%,特异度为99.12%;两方法联合诊断(其中1项阳性即判定为患者)宫颈癌前病变的灵敏度为99.32%,特异度为99.61%.结论:液基细胞检测技术和高危HPV-DNA检测的联合应用优于单项技术检测,对于宫颈癌前病变的筛检具有重要意义.%AIM: To investigate liquid - based cytology technique ( LCT ) combined with high - risk human papilloma virus DNA ( HPV - DNA ) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV - DNA detection in 6 521 women at the age of 19 ~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high - risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results

  10. Microsatellite instability in gastric cancer and pre-cancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Ping Liu; Xiao-Yong Zhang; Yun Shao; Dao-Fu Zhang

    2005-01-01

    AIM: To investigate the microsatellite instability (MSI) in cancer and pre-cancerous lesions of the stomach and its mechanisms underlying the development of gastric cancer.METHODS: Thirty-six gastric cancer samples were obtained from patients undergoing surgery. Forty-one gastric mucosa samples with dysplasia and 51 with intestinal metaplasia (IM) were obtained from patients with chronic gastritis undergoing gastro-endoscopy. Genomic DNA was extracted from the samples. Silver staining single strand conformation polymorphis-polymerize chain reaction (SSCP-PCR) was used to screen MSI markers at 5 loci (Bat-25, Bat-26, D5S346, D17S250, and D2S123)in fresh tissues and formalin-fixed, paraffin-embedded samples and their corresponding normal gastric mucosa.RESULTS: The abnormal shifting of the single-strand DNA (MSI) was identified in 21 out of 36 (58.3%) gastric cancers.Seven cases showed high-level MSI (two or more loci altered) and 14 showed low-level MSI (one locus altered).Gastric cancer with MSI had a tendency to be located in the distal stomach. MSI was also detected in 11 out of 41(26.8%) dysplasia samples and in 9 of 51 (17.6%) IM samples respectively. Three cases of dysplasia and one case of IM showed high-level MSI. Eight cases of dysplasia and 8 cases of IM displayed low-level MSI. MIS in IM was found only in moderate or severe-grade IM. No association was detected between MSI and dysplasia grade.CONCLUSION: Accumulation of MSI in dysplasia and intestinal metaplasia of gastric mucosa may be an early molecular event during gastric carcinogenesis and may contribute to the acquisition of transformed cell phenotype and the development of gastric cancer.

  11. Application of HPV genotype detection in prevention and treatment of cervical cancer and cervical precancerous lesions%人乳头瘤病毒分型检测在宫颈癌及癌前病变防治中的应用

    Institute of Scientific and Technical Information of China (English)

    高忠媛; 何莲芝

    2011-01-01

    Epidemiological studies have shown that human papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasia(CIN).And different types of HPV have different carcinogenicity,different cervical cancer pathological types and prognoses of cervical cancer.The effective way to decrease the cervical cancer morbidity is to detect and classify the high-risk HPV infection in early time,and carry out intervention therapy in early time.Therefore,detecting HPV subtypes in clinic will have great significance in prevention and treatment of cervical cancer and cervical precancerous.%人乳头瘤病毒(HPV)与宫颈癌及宫颈癌前病变的发生密切相关,并且HPV亚型不同,其致癌力、宫颈癌病理分型以及宫颈癌的预后均有所不同.早期发现官颈高危型HPV感染与准确分型,及时进行早期干预治疗,是提高宫颈癌防治效果的有效途径,临床HPV分型检测对宫颈癌及癌前病变的防治有重要意义.

  12. Prevalence of precancerous lesions and conditions in Telangana region, Andhra Pradesh, India

    Directory of Open Access Journals (Sweden)

    B Hari Vinay

    2014-01-01

    Full Text Available Aim: To assess the prevalence of oral precancerous lesions and conditions and to determine the potential risk factors associated among general population aged 20-70 years of Telangana region, Andhra Pradesh. Materials and Methods: An oral screening campaign was organized with the help of local general dental practitioners in each district. All the relevant information regarding the subject was documented and clinical examination of the oral soft tissues was carried out. Clinically diagnosed as precancerous lesion or condition were subjected to punch (incisional biopsy biopsy and evaluated histopathologically to confirm the diagnosis. Results: Among 1200 screened, 150 subjects were chewers and 136 subjects were smokers with 29 chewers and 16 smokers had precancerous lesions and conditions. An overall prevalence of oral precancerous lesions and conditions were found to be 4.2% (males 5.5%; females 2.5%. Leukoplakia was seen in 0.8%, Oral submucous fibrosis in 1.3% and lichen planus in 2% of the study population. Conclusions: The prevalence of precancerous lesions and conditions in our study is higher when compared with the reports from different parts of the country and in South East Asia. These lesions can be prevented from malignant transformation by mass screening, close monitoring, early detection, appropriate treatment plan and prognosis.

  13. Os Odontoideum: Rare Cervical Lesion

    Science.gov (United States)

    2011-11-01

    the articulation between C1 and the os odontoideum on flexion imaging. The remainder of his cervical vertebral bodies had normal alignment with no...appears normal. Figure 3. Flexion view of plain cervical spine. This image shows abnormal translation of the articulation between C1 and the C2 os...worldwide. Peer Reviewed Title: Os Odontoideum: Rare Cervical Lesion Journal Issue: Western Journal of Emergency Medicine, 12(4) Author: Robson

  14. Long-term outcomes of endoscopic argon plasma coagulation (APC) therapy for early esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    王国清

    2013-01-01

    Objective To evaluate the long-term outcomes of endoscopic argon plasma coagulation (APC) therapy for early esophageal cancer and precancerous lesions.Methods One-hundred and seventy one cases with early esophageal cancer (intramucosal carcinoma) and precancerous lesions were treated by APC from 1994 to 2005,

  15. Effect of Helicobacter pylori infection on Bax protein expression in patients with gastric precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Hai-Feng Liu; Wei-Wen Liu; Guo-An Wang; Xiao-Chun Teng

    2005-01-01

    AIM: To investigate the effect of Helicobacter pylori (H pylori) infection on Bax protein expression, and explore the role of H pylori in gastric carcinogenesis.METHODS: H pylori was assessed by rapid urease test and Warthin-Starry method, and expression of Bax protein was examined immunohistochemically in 72 patients with pre-malignant lesions.RESULTS: Bax protein was differently expressed in intestinal metaplasia and gastric dysplasia, and showed 63.99% positivity. The positivity of Bax protein expression in H pylori-positive gastric precancerous lesions (72.3%) was significantly higher than that in H pylori-negative gastric precancerous lesions (48.0%, χ2 = 4.191, P<0.05).H pylori infection was well correlated with the expression of Bax protein in gastric precancerous lesions (r = 0.978,P<0.01). After eradication of H pylori, the positivity of Bax protein expression significantly decreased in H pylori-positive gastric precancerous lesions (χ2= 5.506,P<0.05). In the persisting H pylori-infected patients,the positivity of Bax protein expression was not changed.CONCLUSION: H pylori infection may be involved in the upregulation of Bax gene, which might be one of the mechanisms of H pylori infection-induced gastric epithelial cell apoptosis. H pylori might act as a tumor promoter in the genesis of gastric carcinoma and eradication of H pylori could inhibit gastric carcinogenesis.

  16. 高危型HPV检查和TCT在宫颈癌及癌前病变的早期诊断临床研究%Clinical studies of high-risk HPV examination and TCT in the early diagnosis of cervical cancer and precancerous lesions.

    Institute of Scientific and Technical Information of China (English)

    董静; 倪爱军

    2013-01-01

    Objective To investigate the clinical value of the high - risk types of human papillomavims ( HPV ) examination and thinprep cytologic test ( TCT ) in the early diagnosis of cervical cancer and precancerous lesions. Methods High - risk HPV and TCT examination results in 1248 women screened for cervical cancer were retrospectively analyzed. The clinical diagnostic value of high - risk HPV and TCT examination and the combination of the two methods was compared. Results High - risk HPV infection rate and TCT - positive rate in cervical intraepithelial neo-plasia ( CIN )Ⅰ group, CIN Ⅱ group, CIN Ⅲ group, and cervical cancer group were significantly higher than those in normal and inflammatory group (all P <0. 05 ). The sensitivity, correct diagnosis index, negative predictive value and positive predictive value of the combined detection improved to varying degrees and they were 92. 2% , 0.71, 99. 6% and 40. 2% , respectively. Conclusion The combined detection of high - risk HPV and TCT has important clinical significance for the early diagnosis of cervical cancer.%目的 研究高危型人乳头瘤病毒(HPV)检查及薄层液基细胞学技术(TCT)在宫颈癌与癌前病变的早期诊断的临床应用价值.方法 回顾性分析高危型HPV检查及TCT对1 248例宫颈癌筛查对象的检查结果.比较两种方法及联合应用的临床诊断价值.结果 病理组织学CINⅠ期组、CINⅡ期组、CINⅢ期组、宫颈癌组高危型HPV感染率及TCT阳性率与正常及炎症组比较均有统计学差异(P<0.05);CINⅠ-Ⅲ期及宫颈癌组高危型HPV感染率、TCT阳性率均高于正常及炎症组;高危型HPV检测与TCT两种方法联合检测灵敏度(92.2%),正确诊断指数(0.71)、阴性预测值(99.6%)及阳性预测值(40.2%)均有不同程度的提高.结论 高危型HPV检测与TCT联合对宫颈癌早期发现、早期诊断有重要的临床意义.

  17. Anti- Helicobacter pylori therapy followed by celecoxib on progression of gastric precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Li-Jing Zhang; Shi-Yan Wang; Xiao-Hui Huo; Zhen-Long Zhu; Jian-Kun Chu; Jin-Cheng Ma; Dong-Sheng Cui; Ping Gu; Zeng-Ren Zhao; Ming-Wei Wang; Jun Yu

    2009-01-01

    AIM:To evaluate whether celecoxib,a selective cyclooxygenase 2 (COX-2) inhibitor,could reduce the severity of gastric precancerous lesions following Helicobacter pylori (H pylori) eradication.METHODS:H pylori-eradicated patients with gastric precancerous lesions randomly received either celecoxib (n=30) or placebo (n=30) for up to 3 mo.COX-2 expression and activity was determined by immunostaining and prostaglandin E2 (PGE2) assay,cell proliferation by Ki-67 immunostaining,apoptosis by TUNEL staining and angiogenesis by microvascular density (MVD) assay using CD31 staining.RESULTS:COX-2 protein expression was significantly increased in gastric precancerous lesions (atrophy,intestinal metaplasia and dysplasia,respectively) compared with chronic gastritis,and was concomitant with an increase in cell proliferation and angiogenesis.A significant improvement in precancerous lesions was observed in patients who received celecoxib compared with those who received placebo (P<0.001).Of these three changes,84.6% of sites with dysplasia regressed in patients treated with celecoxib (P=0.002) compared with 60% in the placebo group,suggesting that celecoxib was effective on the regression of dysplasia.COX-2 protein expression (P<0.001) and COX-2 activity (P<0.001) in the gastric tissues were consistently lower in celecoxib-treated patients compared with the placebo-treated subjects.Moreover,it was also shown that celecoxib suppressed cell proliferation (P<0.01),induced cell apoptosis (P<0.01) and inhibited angiogenesis with decreased MVD (P<0.001).However,all of these effects were not seen in placebo-treated subjects.Furthermore,COX-2 inhibition resulted in the up-regulation of PPARg expression,a protective molecule with anti-neoplastic effects.CONCLUSION:H pylori eradication therapy followed by celecoxib treatment improves gastric precancerous lesions by inhibiting COX-2 activity,inducing apoptosis,and suppressing cell proliferation and angiogenesis.

  18. Clinical significance of fluorescence in situ hybridization for detection of hTERC gene amplification in cervical cancer and precancerous tissues cases

    Directory of Open Access Journals (Sweden)

    Shuang LIU

    2012-06-01

    Full Text Available Objective  To detect the human telomerase RNA gene (hTERC amplification in cervical lesions, and explore its clinical significance. Methods  The tissues of the cervical lesions were collected from 195 patients, including 33 of chronic cervicitis, 34 of CINⅠ, 37 of CIN Ⅱ-Ⅲ, 30 of cervical squamous cell carcinoma, and 61 of cervica1 adenocarcinoma, and abnormal hTERC was detected with amplification of fluorescence in situhybridization (FISH. The relationship between hTERC gene amplification and clinicopathological parameters was analyzed. Results  Among the 195 patients, the positive rate of hTERC gene amplification was 3.03% (1/33, 29.41% (10/34, 72.97% (27/37, 100% (30/30, 91.8% (56/61 in chronic cervicitis, CINⅠ, CIN Ⅱ-Ⅲ, cervical squamous cell carcinoma and cervica1 adenocarcinoma respectively, and the results showed that hTERC amplification rate was significantly higher in group CIN Ⅱ-Ⅲthan in group CINⅠ(P 0.05. Conclusion  Detection of gene amplification by FISH technology can be used as a means for accurate diagnosis and prediction of the histologically difficult-to-diagnose lesion and for risk assessment after treatment of cervical precancerous lesions.

  19. Clinical value of high-risk HPV nucleic acid detection kit careHPV in screening of cervical cancer and precancerous lesions%高危型人乳头瘤病毒核酸检测试剂盒-Care HPV用于宫颈癌及癌前病变筛查的临床价值

    Institute of Scientific and Technical Information of China (English)

    方婧; 洪颖

    2016-01-01

    Objective To assess the clinical value of careHPV test in screening of cervical cancer and precancerous lesions.Methods From 2010 to 2012 cervical specimens were collected from women aged 30-59 years old visiting department of gynecology of Nanjing Drum Tower Hospital between.They were detected by careHPV test, HC2, HPV-PCR test and TCT.Women with TCT≥ASC-US had colposcopy with directed biopsy and endocervical curettage if necessary.Based on the gold standard of histopathology, patients with CIN2 or higher belonged to high-risk group and those with inflammation/CIN1 belonged to benign group.Results Totally 404 valid samples were collected. There were 42 cases in high-risk group, 50 cases in benign group, and 312 cases in normal group.The value of Kappa was 0.833 in indicating the consistency of detection results between careHPV test and HC2 (P<0.0001) and the concordance rate was 93.81%.The value of Kappa was 0.707 in indicating the consistency of detection results between careHPV test and HPV-PCR (P<0.0001) and the concordance rate was 88.12%.The sensitivity and specificity of careHPV test in detecting lesions≥CIN 2 were 85.71% and 83.15%, respectively.Conclusion The careHPV test is highly consistent with HC2 and HPV-PCR test.CareHPV has good sensitivity and specificity in the detection of HPV infection with short time consuming, and is expected to be the main method for screening of cervical cancer and precancerous lesions in the areas with relatively insufficient medical resources.%目的 评价care HPV用于宫颈癌及癌前病变筛查的临床价值.方法 收集2010年至2012年南京鼓楼医院妇科就诊的年龄在30~59岁之间的妇女宫颈样本,行care HPV、第二代杂交捕获技术(HC2)、HPV聚合酶链反应(HPV-PCR)及宫颈液基细胞学(TCT)检查,TCT≥ASC-US者行阴道镜宫颈活检及必要时宫颈管诊刮,以组织病理学为金标准.病理诊断≥CIN2者为高危病变组,炎症/CIN 1为良性病变组.结果 共收集404

  20. Oral precancerous lesions: Problems of early detection and oral cancer prevention

    Science.gov (United States)

    Gileva, Olga S.; Libik, Tatiana V.; Danilov, Konstantin V.

    2016-08-01

    The study presents the results of the research in the structure, local and systemic risk factors, peculiarities of the clinical manifestation, and quality of primary diagnosis of precancerous oral mucosa lesions (OMLs). In the study a wide range of OMLs and high (25.4%) proportion of oral precancerous lesions (OPLs) in their structure was indicated. The high percentage of different diagnostic errors and the lack of oncological awareness of dental practitioners, as well as the sharp necessity of inclusion of precancer/cancer early detection techniques into their daily practice were noted. The effectiveness of chemilumenescence system of early OPLs and oral cancer detection was demonstrated, the prospects of infrared thermography as a diagnostic tool were also discussed.

  1. Study on Translational Medicine of RUNX3 Expression in Oral Precancerous Lesions and Squamous Cell Carcinoma

    Directory of Open Access Journals (Sweden)

    Ramamurthy Mustafa

    2016-03-01

    Full Text Available Objective: To explore the expression and distribution characteristics of RUNX3 in the pathological process of oralsquamous cell carcinoma (OSCC as well as its correlation with clinicopathological features. Methods: Fifteen cases of normal oral mucosal tissues, 37 cases of OSCC tissues and 21 cases of precancerous lesion tissues were respectively collected. The expression and distribution characteristics of RUNX3 were detected using immunohistochemical method, and its relationship with each clinicopathological feature of OSCC was analyzed. Results: The positive rates of RUNX3 expression in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues were 100% (15/15, 95.24% (20/21 and 54.05% (20/37, and their average optical densities were (0.391±0.07, (0.369±0.03 and (0.263±0.01, respectively. The differences were statistically significant by comparison to the positive rates of RUNX3 expression and their average optical densities in normal oral mucosal tissue, precancerous lesion tissue and OSCC tissue (P<0.01. There was no statistical significance regarding RUNX3 expression in the patients with different gender, age and presence or absence of lymph node metastasis (P>0.05, but the difference was statistically significant among the patients with different pathological gradings (P=0.012. Conclusion: The expression of RUNX3 in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues gradually shows a down-regulating tendency and its frequency of abnormal localization is on the rise. The expression of RUNX3 is closely associated with the degree of histological differentiation. All of these findings indicate that RUNX3, an important action factor in the occurrence and progression of OSCC, can be considered as a key biological indicator for early diagnosis, judgment of malignant degree and prognostic monitoring.

  2. Study on Translational Medicine of RUNX3 Expression in Oral Precancerous Lesions and Squamous Cell Carcinoma

    Institute of Scientific and Technical Information of China (English)

    Ramamurthy Mustafa; Shabil Mohamed Rajaraman

    2016-01-01

    Objective: To explore the expression and distribution characteristics of RUNX3 in the pathological process of oral squamous cell carcinoma (OSCC) as well as its correlation with clinicopathological features. Methods: Fifteen cases of normal oral mucosal tissues, 37 cases of OSCC tissues and 21 cases of precancerous lesion tissues were respectively collected. The expression and distribution characteristics of RUNX3 were detected using immunohistochemical method, and its relationship with each clinicopathological feature of OSCC was analyzed. Results: The positive rates of RUNX3 expression in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues were 100% (15/15), 95.24% (20/21) and 54.05% (20/37), and their average optical densities were (0.391±0.07), (0.369±0.03) and (0.263±0.01), respectively. The differences were statistically signiifcant by comparison to the positive rates of RUNX3 expression and their average optical densities in normal oral mucosal tissue, precancerous lesion tissue and OSCC tissue (P0.05), but the difference was statistically signiifcant among the patients with different pathological gradings (P=0.012). Conclusion: The expression of RUNX3 in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues gradually shows a down-regulating tendency and its frequency of abnormal localization is on the rise. The expression of RUNX3 is closely associated with the degree of histological differentiation. All of these findings indicate that RUNX3, an important action factor in the occurrence and progression of OSCC, can be considered as a key biological indicator for early diagnosis, judgment of malignant degree and prognostic monitoring.

  3. Conifer Green Needle Complex in Patients with Precancerous Gastric Lesions: An Observational Pilot Study

    Directory of Open Access Journals (Sweden)

    Vladimir Bespalov

    2016-01-01

    Full Text Available Objectives. Helicobacter pylori infection is common and can lead to precancerous gastric lesions. Standard antibiotic therapy has a failure rate of more than 25% from antibiotic resistance. The primary aim of this observational pilot study was to test the feasibility of a large-scale clinical trial of Conifer Green Needle Complex (CGNC to treat precancerous gastric lesions. Secondary aims were to investigate H. pylori infection, stomach function, and histopathology of the gastric mucosa. Methods. A tablet form of CGNC (extracted from Pinus sylvestris and Picea abies (L Karst was prescribed to 26 patients with precancerous gastric lesions (two tablets, 100 mg CGNC/tablet, three times per day for six months. Another 24 patients received no treatment. Results. Compared with control patients, CGNC-treated patients showed total or partial regression (using the quantitative Rome III diagnostic criteria of dyspeptic symptoms (92.3%, p<0.0001, eradication of H. pylori infection (57.1%, p<0.03, a reduction in endoscopic signs of gastritis (92.3%, p<0.001, an increase of pepsinogen-pepsin in the gastric juice (57.7%, p<0.05, and total regression or reduction in the degree of intestinal metaplasia (46.2%, p<0.05 and lymphoplasmacytic infiltration (53.8%, p<0.05. Conclusions. This study justifies a randomised-controlled trial with CGNC in patients with atrophic gastritis.

  4. Glycoproteomic analysis of serum from patients with gastric precancerous lesions

    DEFF Research Database (Denmark)

    Gomes, Catarina; Almeida, Andreia; Ferreira, José Alexandre

    2013-01-01

    Gastric cancer is preceded by a carcinogenesis pathway that includes gastritis caused by Helicobacter pylori infection, chronic atrophic gastritis that may progress to intestinal metaplasia (IM), dysplasia, and ultimately gastric carcinoma of the more common intestinal subtype. The identification...... of glycosylation changes in circulating serum proteins in patients with precursor lesions of gastric cancer is of high interest and represents a source of putative new biomarkers for early diagnosis and intervention. This study applies a glycoproteomic approach to identify altered glycoproteins expressing...... the simple mucin-type carbohydrate antigens T and STn in the serum of patients with gastritis, IM (complete and incomplete subtypes), and control healthy individuals. The immunohistochemistry analysis of the gastric mucosa of these patients showed expression of T and STn antigens in gastric lesions, with STn...

  5. Narrow-band imaging in the diagnosis of early esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    HUANG Liu-ye; CUI Jun; WU Cheng-rong; LIU Yun-xiang; XU Ning

    2009-01-01

    Background In the recent years,the incidence of esophageal cancer in China has increased.The key point for raising the survival rate is the diagnosis and treatment at an early stage.Narrow-band imaging (NBI) can enhance the contrast of the mucous membrane of the esophagus without staining.This study aimed to explore the value of NBI in the diagnosis of early esophageal cancer and precancerous lesions.Methods The esophagus was examined with ordinary endoscopy and NBI endoscopy.Pit patterns and blood capillary forms were examined with routine magnifying endoscopy and NBI endoscopy.Finally,a 1.2% Lugoul's iodine solution was used to stain the esophageal mucosal surface and a biopsy was taken at all the sites where NBI or iodine staining was positive.NBI and iodine staining scales were compared with pathologic diagnosis,which was considered as the gold standard.Results A total of 90 cases (138 lesions in total) were diagnosed as early esophageal cancer or precancerous lesions:104 lesions (75.4%) were detected with ordinary endoscopy,120 lesions (87.0%) were detected with NBI endoscopy,and 138 lesions (100%) were detected with iodine staining.The lesion detection rate of NBI was significantly lower than that of iodine staining (X2=17.176,P <0.01).However,there was no significant difference between NBI and iodine staining for the diagnosis of high grade intraepithelial neoplasia (X2=1.362,P >0.05),while the detection rate of NBI was significantly lower than that of iodine staining for the diagnosis of low grade intraepithelial neoplasia (X2=13.388,P <0.01).The pit pattern and blood capillary form of eady esophageal cancer and precancerous lesions could be demonstrated clearer with NBI than with ordinary endoscopy.Conclusions NBI can enhance the contrast of the mucous membrane of the esophagus without staining.The combination of NBI and iodine staining can raise the diagnostic rate of early esophageal cancer and precancerous lesions.

  6. LEEP术在宫颈癌前病变应用中的研究进展%The progression of loop electrosurgical excision procedure surgery in cervical precancerous lesions application

    Institute of Scientific and Technical Information of China (English)

    李彩虹

    2013-01-01

    近年来,随着宫颈癌筛查水平的不断提高,其发病率逐年下降,但宫颈癌前病变的检出率逐年上升并呈现出年轻化的趋势.如何有效地诊治宫颈癌前病变,成为广大妇科医师极为关注的话题.对于宫颈细胞学检查异常的患者,其处理方式已由单纯的冷刀锥切、电灼到冷冻等手术直至现在的高频电波刀宫颈环切术(Loop electrosurgical excision procedure,LEEP).LEEP 治疗宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)效果已得到广大学者和临床医师认可,治疗也日臻完美,然而随着对此项技术的深入研究,LEEP术在宫颈癌前病变某些诊治环节中不同观点日渐凸显出来,因此本文将主要针对有争议的术后妊娠结局、复发高危因素等进行综述.

  7. 血浆sHLA-G在宫颈癌和癌前病变检测中的应用%The application of plasma soluble human leukocyte antigen in diagnosis of patients with cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    张欣; 郑妮; 王传新; 朱成宝; 王丽丽; 杜鲁涛; 王顺

    2011-01-01

    康对照组(U值分别为7.564、4.522、7.429,P均<0.05);CIN Ⅲ级组血浆CA125水平明显高于CIN Ⅰ级组和健康对照组(U值分别为5.871、5.435,P均<0.05).ROC曲线分析sHLA-G的AUC为0.828(95% CI:0.768~0.879),其诊断价值高于SCC-Ag[0.727(95% CI:0.658~0.788);Z=2.294,P<0.05]和CA125[0.705(95%CI:0.636~0.769);Z=2.842,P<0.05];SCC-Ag与CA125的诊断效能比较,差异无统计学意义(Z=0.672,P>0.05).经ROC曲线分析确定sHLA-G诊断临界值为109.6 kU/L时,其敏感度为86.3%,特异度为76.1%,阳性预测值为80.0%,阴性预测值为83.3%,准确性为78.4%.宫颈癌患者血浆sHLA-G水平与FIGO分期及淋巴结转移密切相关(U值分别为6.085、4.451,P均<0.05),而与患者年龄、肿瘤大小、组织形态及细胞分化无关(U值分别为1.274、1.956、1.268、2.719,P均>0.05).结论 血浆sHLA-G水平检测有助于宫颈癌及癌前病变的早期发现,并且可以作为宫颈癌病情进展及淋巴结转移的判断指标.%Objective To explore the application value of plasma sHLA-G in diagnosis of CIN and cervical cancer. Methods The plasma sHLA-G levels were detected by ELISA in 102 cases with cervical cancer( FIGO Ⅰ stage 32 cases, Ⅱ stage 28 cases, Ⅲ stage 25 cases and Ⅳstage 17 cases; tumor size:<4 cm 63 cases and ≥4 cm 39 cases; squamous cell carcinoma 78 cases and adenocarcinoma 24 cases;cell differentiation:well 57 cases, moderate 29 cases and poor 16 cases; lymph nodes metastasis negative64 cases and positive 38 cases ), 72 cases with CIN( Ⅰ grade 21 cases, Ⅱ grade 25 cases and Ⅲ grade26 cases ) and 20 cases of healthy controls. The diagnostic value of sHLA-G and its correlations with clinical parameters were analyzed. Results The plasma levels of sHLA-G were 193.6( 151.3-287.4 ) kU/L in cervical cancer group, 48.3( 34.6-57.2 ) kU/L in CIN Ⅰ group, 91.3( 68.2-118.6 ) kU/L in CIN Ⅱ group, 106.4( 73.8-165.7 ) kU/L in CIN Ⅲ group and 45.2( 38.0-55.5 ) kU/L in health control group.The level of s

  8. Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Ming-Yao Wu; Ying-Rui Liang; Xian-Ying Wu; Chu-Xiang Zhuang

    2002-01-01

    AIM: To study the expression of early growth response gene1 (Egr-1 gene) and Bcl-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC) and precancerous lesions.METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa.RESULTS: Egr-1 gene in situ hybridization, Bcl-X/L immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01).The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC.CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.

  9. Characterization of breast precancerous lesions and myoepithelial hyperplasia in sclerosing adenosis with apocrine metaplasia

    DEFF Research Database (Denmark)

    Celis, J.E.; Gromova, I.; Cabezón, T.;

    2007-01-01

    . First, we identify specific protein biomarkers for benign apocrine metaplasia and thereafter we search for biomarkers that are highly overexpressed by pure invasive apocrine carcinomas. Here we present studies in which we have used antibodies against components of a benign apocrine signature...... the three markers associated with pure invasive apocrine carcinomas. These studies also revealed p53 positive, non-apocrine putative precancerous lesions as well as novel phenotypes for ME and some luminal cells characterized by the expression of cytokeratin 15. © 2007 Federation of European Biochemical...

  10. A preliminary study on ras protein expression in human esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Jian Li; Chang Wei Feng; Zhi Guo Zhao; Qi Zhou; Li Dong Wang

    2000-01-01

    @@INTRODUCTION The esophageal carcinoma is a common malignant tumor in Linzhou City (Linxian) of Henan Province in northern China. Although the etiology and natural history of esophageal carcinoma are not clear, a substantial amount of evidence has been provided to suggest that the development of human esophageal squamous cell carcinomas (SCC) is a multistage progressive process[1-4] An early indicator of abnormality in persons predisposed to esophageal SCC is an increased proliferation of esophageal epithelial cells,morphologically manifested as basal cell hyperplasia (BCH), and dysplasia (DYS), and carcinoma in situ, which could be considered precancerous lesions of esophageal SCC[1-4].

  11. A STUDY ON THE ROLE OF MICRO NUCLEI IN ASSESSING THE PROGRESSION OF PRECANCEROUS LESIONS OF CERVIX AND THE DIAGNOSIS OF CARCINOMA OF CERVIX

    Directory of Open Access Journals (Sweden)

    Anitha

    2015-06-01

    Full Text Available BACKGROUND: Invasive squamous cell carcinoma of cervix remains the most common malignant neoplasm of the female genital tract in many countries. The Papanicolaou stained cervical smear is an excellent and effective method in the diagnosis of invasive carcinoma and precancerous lesions of the cervix. This study was conducted to assess the value of Feulgen stained cervical smears in elucidating nuclear features help ful in the diagnosis of malignancy over conventional Pap stained smears and also to look for micronucleated cells in suspected cases of carcinoma of cervix. AIM: To analyse the distribution of cases of invasive squamous cell carcinoma and intraepithelial n eoplasia (squamous intraepithelial lesion of cervix over a period of 3 years, to elucidate additional nuclear features helpful in diagnosis of carcinoma using Feulgen stained cervical smears and to study the distribution of micronuclei in Feulgen stained smears from suspected cases of Carcinoma cervix. MATERIALS AND METHODS: A three year analysis of all cases of dysplasia and invasive carcinoma of cervix was done by reviewing Papanicolaou stained cervical smears from all the diagnosed cases of invasive car cinoma and precancerous lesions of the cervix. Cervical smears from sixty clinically suspected cases of carcinoma of cervix and smears from 10 normal women collected during a period of 12 months were studied in detail using Papanicolaou stained and Feulgen Stained Smears and micronuclei analysis (MN analysis was done. RESULTS : A total of 24343 cervical smears were screened during the 3 year period of study. Out of these 24343 cases there were 267(1.09% cases of cervical intraepithelial neoplasia and 144(0 .592% cases of invasive squamous cell carcinoma. Micronuclei analysis done using Feulgen Stained Smears demonstrated a consistent increase in micronucleated cells proportional to the increase in severity of th e lesion from normal to invasive carcinoma. CO NCLUSION : This study

  12. Secondary prevention of cervical cancer through the development and implementation of a system to optimize diagnostic and therapeutic and rehabilitation measures in the background and precancerous cervical diseases

    Directory of Open Access Journals (Sweden)

    F. F. Badretdinova

    2012-01-01

    Full Text Available The results of a comprehensive evaluation and treatment of background and pre-cancerous cervical cancer of women were studied (n = 1022. There is the complex assessment of social and obstetric gynecological risk factors for cervical intraepithelial neoplasia and cervical cancer. A system for optimizing diagnostic, therapeutic, preventive and rehabilitative measures, taking into account the differentiated approach to the choice of treatment, follow-up in the near and long-term postoperative period. An individual approach to the selection of organ presentation or radical treatment using new technologies of surgical treatment are identified. Application of the developed system enabled a statistically significantly improve the results of treatment of background and precancerous cervical disease.

  13. THE ROLE OF HELICOBACTER PYLORI IN GASTRIC CANCER AND PRECANCEROUS LESIONS

    Institute of Scientific and Technical Information of China (English)

    任刚; 蔡嵘; 陈强; 许幼如; 张文竹; 奚政君

    2002-01-01

    Objective To determine the role of Helicobacter pylori ( Hp ) in the development of gastric cancer and gastric precancerous lesions. Methods From the biopsy specintestinal metaplasiaens of gastric cancer and precancerous lesions Hp organisms were detected by a combination use of three methods ( Clo-test,Giemsa and PCR ). Mutations of C-Ha-ras and p53 genes were determined by polymerase chain reaction-single strand conformation polymorphism analysis-direct sequencing ( PCR-SSCP-S ). Results The detection rates of Hp in gastric cancer, dysplasia and intestinal metaplasia were significantly higher than that of normal gastric mucosa, and the mutational rates of C-Ha-ras and p53 genes in Hp positive patients were significantly higher than those in Hp negative patients. In gastric cancer patients, the mutational rate of p53 gene in Hp positive patients was significantly higher than that of Hp negative patients, in which spot mutation was the main pattern of gene changes. Conclusion Hp infection might be related to gastrocarcinogenesis and gene mutation might play a role in Hp related gastric cancer.

  14. Optical detection of cancer and precancerous lesions of the upper aerodigestive tract: methods for assessment of vertical extensions

    Science.gov (United States)

    Kraft, Marcel; Arens, Christoph

    2013-06-01

    Introduction: Endoscopic imaging can be used in the assessment of cancer and precancerous lesions of the upper aerodigestive tract. The objective of this presentation is to describe vertical imaging methods. Methods: The working principle and technique of optical coherence tomography (OCT) as well as endosonography (EUS) are briefly illustrated. Results: The main indications, normal and pathologic findings of each method, frequent pitfalls, and clinical results up to now are presented in detail. Conclusions: Endosonography helps in determining the exact extension of advanced carcinomas beyond 3 mm, while OCT, due to its better resolution, is superior in the assessment of precancerous and early cancerous lesions up to 2 mm thickness.

  15. In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix

    Energy Technology Data Exchange (ETDEWEB)

    Mourant, Judith R [Los Alamos National Laboratory

    2008-01-01

    A noninvasive optical diagnostic system for detection of cancerous and precancerous lesions of the cervix was evaluated in vivo. The optical system included a fiber-optic probe designed to measure polarized and unpolarized light transport properties of a small volume of tissue. An algorithm for diagnosing tissue based on the optical measurements was developed that used four optical properties, three of which were related to light scattering properties and the fourth of which was related to hemoglobin concentration. A sensitivity of {approx}77% and specificities in the mid 60% range were obtained for separating high grade squamous intraepithelial lesions and cancer from other pathologies and normal tissue. The use of different cross-validation methods in algorithm development is analyzed, and the relative difficulties of diagnosing certain pathologies are assessed. Furthermore, the robustness of the optical system for use by different doctors and to changes in fiber-optic probe are also assessed, and potential improvements in the optical system are discussed.

  16. Assessment of hemodynamics in precancerous lesion of hepatocellular carcinoma: Evaluation with MR perfusion

    Institute of Scientific and Technical Information of China (English)

    Sheng Guan; Wei-Dong Zhao; Kang-Rong Zhou; Wei-Jun Peng; Feng Tang; Jian Mao

    2007-01-01

    AIM: To investigate the hemodynamic changes in a precancerous lesion model of hepatocellular carcinoma (HCC).METHODS: Hemodynamic changes in 18 Wistar rats were studied with non-invasive magnetic resonance (MR)perfusion. The changes induced by diethylnitrosamine (DEN) developed into liver nodular lesions due to hepatic cirrhosis during the progression of carcinogenesis.The MR perfusion data [positive enhancement integral (PEI)] were compared between the nodular lesions corresponding well with MR images and pathology and their surrounding hepatic parenchyma.RESULTS: A total of 46 nodules were located by MR imaging and autopsy, including 22 dysplastic nodules (DN), 9 regenerative nodules (RN), 10 early HCCs and 5 overt HCCs. Among the 22 DNs, 6 were low-grade DN (LGDN) and 16 were high-grade DN (HGDN). The average PEI of RN, DN, early and overt HCC was 205.67± 31.17, 161.94 ± 20.74, 226.09 ± 34.83, 491.86 ±44.61 respectively, and their liver parenchyma nearby was 204.84 ± 70.19. Comparison of the blood perfusion index between each RN and its surrounding hepatic parenchyma showed no statistically significant difference (P = 0.06). There were significant differences in DN (P = 0.02). During the late hepatic arterial phase, the perfusion curve in DN declined. DN had an iso-signal intensity at the early hepatic arterial phase and a low signal intensity at the portal venous phase. Of the 10early HCCs, 4 demonstrated less blood perfusion and 6 displayed minimally increased blood flow compared to the surrounding parenchyma. Five HCCs showed significantly increased blood supply compared to the surrounding parenchyma (P = 0.02).CONCLUSION: Non-invasive MR perfusion can detect changes in blood supply of precancerous lesions.

  17. Dynamic expression of pepsinogen C in gastric cancer, precancerous lesions and Helicobacter pylori associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Pei-Fang Ning; Hui-Jie Liu; Yuan Yuan

    2005-01-01

    AIM: To investigate the relationship between the expression of pepsinogen C (PGC) and gastric cancer, precancerous diseases, and Helicobacter pylori ( H pylori) infection. METHODS: The expression of PGC was determined by immunohistochemistry method in 430 cases of gastric mucosa. H pylori infection was determined by HE staining, PCR and ELISA in 318 specimens.RESULTS: The positive rate of PGC expression in 54 cases of normal gastric mucosa was 100%. The positive rates of PGC expression in superficial gastritis or gastric ulcer or erosion, atrophic gastritis or gastric dysplasia and gastric cancer decreased significantly in sequence (P<0.05;100%/89.2% vs 14.3%/15.2% vs 2.4%). The overexpression rate of PGC in group of superficial gastritis with H pylori infection was higher than that in group without H pylori infection (P<0.05; χ2= 0.032 28/33 vs 15/25).The positive rate of PGC expression in group of atrophic gastritis with H pylori infection was lower than that in group without H pylori infection (P<0.01; χ2 = 0.003 4/61vs 9/30), and in dysplasia and gastric cancer. CONCLUSION: The level of PGC expression has a close relationship with the degree of malignancy of gastric mucosa and development of gastric lesions. There is a relationship between H pylori infection and expression of antigen PGC in gastric mucosa, the positive rate of PGC expression increases in early stage of gastric lesions with H pylori infection such as gastric inflammation and decreases during the late stage such as precancerous diseases and gastric cancer. PGC-negative cases with H pylori-positive gastric lesions should be given special attention.

  18. 液基细胞学联合HPV基因分型检测在宫颈癌前病变筛查中的应用探讨%Discussion on the Application of Liquid Based Cytology and HPV Genotyp-ing in the Screening of Cervical Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    李云

    2015-01-01

    Objective To investigate the clinical application value of liquid based cytology combined with human papilloma virus (HPV) genotyping for the screening cervical precancerous lesions. Methods 380 cases with abnormal atypical squamous cells and more detected by liquid based cytology in our hospital from January 2013 to August 2014 were selected. HPV genotyping was per-formed in all the patients. And the results were analyzed comparatively and retrospectively. Results The test results showed that 130 cases with atypical squamous cells and more and positive HPV, which included 78 cases with atypical squamous cells of un-determined significance (ASCUS), 40 cases with low-grade squamous intraepithelial lesion (LSIL), 7 cases with high-grade squa-mous intraepithelial lesion(HSIL), 5 cases with squamous cell carcinoma (SCC), accounting for 60%, 30.77%, 5.38%, 3.85%, re-spectively. Of the 130 cases, 48 cases had high risk HPV, and among them, the number of patients with high risk AUSCS, LISL, HISL, SCCHPV was 22 cases, 17 cases, 4 cases, 5 cases, respectively, accounting for 27.85%, 42.5%, 57.14%, 100%, respective-ly, the results of the study showed that SCC was directly related to high-risk infection of HPV.There were 48 high risk HPV pa-tients with an average age of 43.6, and 82 low risk HPV patients with an average age of 32.7, with statistically significant differ-ence(P<0.05). Conclusion Liquid based cytology combined with HPV genotyping can effectively diagnose the cervical cancer le-sions, and the data are comparatively accurate and reliable, so it is worthy of clinical application and promotion.%目的:探讨分析液基细胞学联合HPV(human papillomavirus 人乳头状瘤病毒)基因分型检测在宫颈癌前病变筛查中的临床应用价值。方法抽取在2013年1月—2014年8月间该院收治的380例经液基细胞学检查有异常的为非典型鳞状上皮细胞及以上的患者,对这些患者进

  19. 血清可溶性白细胞G抗原在宫颈癌及癌前病变的临床诊断意义%Clinical diagnostic value of plasma sHLA-G antigen in cervical cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    田玫玲; 卢庆乐

    2015-01-01

    断指标。但其与发生宫颈癌及癌前病变的特异性分析仍有待进一步的相关研究。%Objective To explore the clinical diagnostic valve of serum soluble human leukocyte antigen‐G (sHLA‐G) in cervical cancer and precancerous lesion cervical intraepithelial neoplasia(CIN) .Methods The serum sHLA‐G level was detected by using ELISA and serum TA‐4 and SCC‐Ag levels were detected by using the light‐emitting electrochemical immunoassay method detec‐ting in 230 cases of cervical carcinoma ,120 cases of CIN and 30 healthy volunteers .The differences among various groups and their relationship with the clinicopathological features of cervical cancer were analyzed .Results (1) The comparison of serum sHLA‐G , TA‐4 and SCC‐Ag levels :there were statistically significant differences in serum sHLA‐G level among various groups (P=0 .000);the serum sHLA‐G level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰgroup , CIN Ⅱ group and CIN Ⅲ group (P=0 .000 ,P=0 .000 ,P=0 .002 ,P=0 .006);which in the CIN Ⅲ group was significantly higher than that in the CIN Ⅰ group and the healthy control group (P=0 .001 ,P=0 .021) .There were statistically significant differences in serum TA‐4 level among various groups (P=0 .006);the serum TA‐4 level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .003 ,P=0 .008 ,P=0 .018);which in the CIN Ⅲgroup was significantly higher than that in the healthy control group and the CIN Ⅰ group (P=0 .023 ,P=0 .031) .The differences of serum SCC‐Ag level among various groups had statistically significant differences (P=0 .000);which in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .000 ,P=0 .001 ,P=0 .007) , and which in the CIN Ⅲ group was significantly higher than that in the healthy

  20. Prevalence of gastric precancerous lesions in Ardabil, a high incidence province for gastric adenocarcinoma in the northwest of Iran

    NARCIS (Netherlands)

    Malekzadeh, R; Sotoudeh, M; Derakhshan, MH; Mikaeli, J; Yazdanbod, A; Merat, S; Yoonessi, A; Tavangar, M; Ardakani, BA; Sotoudehmanesh, R; Pourshams, A; Asgari, AA; Doulatshahi, S; Alizadeh, BZ; Arshi, S; Madjidpoor, A; Moomen, SM; Fleischer, DE

    2004-01-01

    Background/Aims: Ardabil Province, in northwestern Iran, has the highest rate of gastric ( predominantly cardia) adenocarcinoma in Iran. This study aimed to investigate the feasibility of endoscopic screening and to look for associated Helicobacter pylori infection and gastric precancerous lesions.

  1. Prevalence of gastric precancerous lesions in Ardabil, a high incidence province for gastric adenocarcinoma in the northwest of Iran

    NARCIS (Netherlands)

    Malekzadeh, R; Sotoudeh, M; Derakhshan, M H; Mikaeli, J; Yazdanbod, A; Merat, S; Yoonessi, A; Tavangar, M; Abedi, B Ardakani; Sotoudehmanesh, R; Pourshams, A; Asgari, A Ali; Doulatshahi, S; Alizadeh, B Ziad; Arshi, S; Madjidpoor, A; Mir Moomen, S; Fleischer, D E

    2004-01-01

    BACKGROUND/AIMS: Ardabil Province, in northwestern Iran, has the highest rate of gastric (predominantly cardia) adenocarcinoma in Iran. This study aimed to investigate the feasibility of endoscopic screening and to look for associated Helicobacter pylori infection and gastric precancerous lesions. M

  2. Screening for Precancerous Lesions of Upper Gastrointestinal Tract: From the Endoscopists' Viewpoint

    Directory of Open Access Journals (Sweden)

    Chen-Shuan Chung

    2013-01-01

    Full Text Available Upper gastrointestinal tract cancers are one of the most important leading causes of cancer death worldwide. Diagnosis at late stages always brings about poor outcome of these malignancies. The early detection of precancerous or early cancerous lesions of gastrointestinal tract is therefore of utmost importance to improve the overall outcome and maintain a good quality of life of patients. The desire of endoscopists to visualize the invisibles under conventional white-light endoscopy has accelerated the advancements in endoscopy technologies. Nowadays, image-enhanced endoscopy which utilizes optical- or dye-based contrasting techniques has been widely applied in endoscopic screening program of gastrointestinal tract malignancies. These contrasting endoscopic technologies not only improve the visualization of early foci missed by conventional endoscopy, but also gain the insight of histopathology and tumor invasiveness, that is so-called optical biopsy. Here, we will review the application of advanced endoscopy technique in screening program of upper gastrointestinal tract cancers.

  3. Expression and significance of homeodomain protein Cdx2 in gastric carcinoma and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Rong Qin; Na-Na Wang; Jing Chu; Xian Wang

    2012-01-01

    AIM:To investigate the expression and significance of caudal-related homeobox transcription factor (Cdx2) in gastric carcinoma (GC) and precancerous lesions.METHODS:The expression of Cdx2 in GC,precancerous lesions and normal gastric mucosa were detected using immunohistochemical method.Hematoxylin and eosin staining,alcian blue/periodic acid-schiff and high iron diamine/alcian blue staining were used to classify intestinal metaplasia (IM) and GC.RESULTS:Cdx2 was not detected in normal gastric mucosa.Cdx2 expression was detected in 87.1%(101/116) of IM,50% (36/72) of dysplasia and 48.2%(41/85) of GC.The Cdx2-expressing cells in IM were more prevalent than in dysplasia and carcinoma (P <0.05).There was no relationship between Cdx2 expression and the classification of IM or the degree of dysplasia.Expression of Cdx2 was significantly higher in intestinal-type carcinoma than in diffuse and mixedtype carcinoma (P < 0.05).Positive expression of Cdx2 was mainly found in moderately to well differentiated GC.There was a negative association between nuclear Cdx2 expression and lymph node metastasis and tumor,nodes,metastasis stage of GC (P < 0.05).The patients with Cdx2-positive expression showed a higher survival rate than those with Cdx2-negative expression (P =0.038).Multivariate analysis revealed that the expression of Cdx2 and lymph node metastasis were independent prognostic indicators of GC (P < 0.05).CONCLUSION:Cdx2 may be closely related to IM and the intestinal-type GC and implicate better biological behavior and outcome.Cdx2 is useful for predicting the prognosis of GC.

  4. Computer-aided screening system for cervical precancerous cells based on field emission scanning electron microscopy and energy dispersive x-ray images and spectra

    Science.gov (United States)

    Jusman, Yessi; Ng, Siew-Cheok; Hasikin, Khairunnisa; Kurnia, Rahmadi; Osman, Noor Azuan Bin Abu; Teoh, Kean Hooi

    2016-10-01

    The capability of field emission scanning electron microscopy and energy dispersive x-ray spectroscopy (FE-SEM/EDX) to scan material structures at the microlevel and characterize the material with its elemental properties has inspired this research, which has developed an FE-SEM/EDX-based cervical cancer screening system. The developed computer-aided screening system consisted of two parts, which were the automatic features of extraction and classification. For the automatic features extraction algorithm, the image and spectra of cervical cells features extraction algorithm for extracting the discriminant features of FE-SEM/EDX data was introduced. The system automatically extracted two types of features based on FE-SEM/EDX images and FE-SEM/EDX spectra. Textural features were extracted from the FE-SEM/EDX image using a gray level co-occurrence matrix technique, while the FE-SEM/EDX spectra features were calculated based on peak heights and corrected area under the peaks using an algorithm. A discriminant analysis technique was employed to predict the cervical precancerous stage into three classes: normal, low-grade intraepithelial squamous lesion (LSIL), and high-grade intraepithelial squamous lesion (HSIL). The capability of the developed screening system was tested using 700 FE-SEM/EDX spectra (300 normal, 200 LSIL, and 200 HSIL cases). The accuracy, sensitivity, and specificity performances were 98.2%, 99.0%, and 98.0%, respectively.

  5. Diagnostic problems in precancerous lesions and invasive carcinomas of the penis.

    Science.gov (United States)

    Chaux, Alcides; Cubilla, Antonio L

    2012-05-01

    Penile precancerous and invasive lesions exhibit a variegated morphology. Although the diagnosis and classification of penile tumors is straightforward in most cases, a few entities are problematic, especially to pathologists from countries in which penile cancer is rarely encountered. The differential diagnosis of squamous hyperplasias from differentiated penile intraepithelial neoplasia or from extremely low-grade invasive neoplasms (eg, pseudohyperplastic and verrucous carcinomas) may be particularly difficult. Similarly, given the morphologic features shared by all verruciform tumors (ie, verrucous, warty, papillary, and cuniculatum carcinomas, along with giant condylomas), it is challenging at times to distinguish one from another. At the other end of the spectrum, because of their lack of differentiation, it is sometimes difficult to classify high-grade carcinomas, such as basaloid and sarcomatoid, which may have etiologic/prognostic implications. Penile mixed tumors, harboring more than 1 histologic subtype and grade, constitute a frequent finding in routine pathology. The recognition of distinctive morphologic patterns and histologic grades in these tumors is important because these features could be related to etiologic factors, such as human papillomavirus infection, or they could influence outcome. Penile tumors with glandular features (eg, adenosquamous and mucoepidermoid carcinomas), although rare, may be confused with the more common pseudoglandular (adenoid, acantholytic) variant of squamous cell carcinomas, their main mimicker. In this review we provide clues that may help in the differential diagnosis of these lesions.

  6. Therapeutic vaccination with MVA E2 can eliminate precancerous lesions (CIN 1, CIN 2, and CIN 3) associated with infection by oncogenic human papillomavirus.

    Science.gov (United States)

    Corona Gutierrez, Carlos Manuel; Tinoco, Alberto; Navarro, Tania; Contreras, Mario López; Cortes, Roberto Risco; Calzado, Patricia; Reyes, Lise; Posternak, Roberto; Morosoli, Gianni; Verde, Mauro Lara; Rosales, Ricardo

    2004-05-01

    Human papillomavirus (HPV) infection is associated with cervical cancer. Papillomaviruses can induce diseases ranging from warts and condylomata to lesions that can progress to malignant neoplasias. Cervical cancer is a serious problem in developing countries because it is usually not detected at an early stage. In Mexico, a woman dies every 2 hr from this malignancy. In a phase I/II clinical trial, we evaluated the potential use of the MVA E2 recombinant vaccinia virus to treat cervical intraepithelial neoplasia CIN 1, CIN 2, and CIN 3 lesions associated with human papillomavirus (HPV) infection. Seventy-eight women with CIN 1-, CIN 2-, and CIN 3-grade lesions were treated with either an MVA E2 recombinant virus vaccine or with cryosurgery. Thirty-six women received the recombinant virus vaccine at a total of 10(7) MVA E2 virus particles injected directly into the uterus once every week over a 6-week period. Forty-two patients were treated with cryosurgery. Reduction of lesions was monitored weekly by colposcopy and cytologic analysis. The type of immune response after MVA E2 injection was determined by measuring antibody titers against MVA E2 virus and the E2 protein, and by the presence of cytotoxic activity against cancer cells bearing papillomavirus DNA. The presence of papillomavirus was determined by with the hybrid capture method. Thirty-four of 36 patients showed complete elimination of precancerous lesions after treatment with the MVA E2 vaccine. In two patients, precancerous lesions were reduced from grade CIN 3 to CIN 1. Three other patients presented isolated koilocytes after treatment with MVA E2. Colposcopy revealed no lesions in 85% of patients, and only small aceto-white spots were detected in 15% of patients after treatment with MVA E2. All patients developed antibodies against the MVA E2 vaccine, and vaccination generated a specific cytotoxic response against HPV-transformed cells. Furthermore, 50% of patients showed no evidence of papillomavirus

  7. Cervical Cancer

    Centers for Disease Control (CDC) Podcasts

    2007-03-06

    Did you know that cervical cancer rates differ by race/ethnicity and region? Or that cervical cancer can usually be prevented if precancerous cervical lesions are found by a Pap test and treated? Find out how getting regular Pap tests can save a woman's life.  Created: 3/6/2007 by National Breast and Cervical Cancer Early Detection Program.   Date Released: 4/25/2007.

  8. Os Odontoideum: Rare Cervical Lesion

    Directory of Open Access Journals (Sweden)

    Kristie A Robson

    2011-05-01

    Full Text Available We report the case of a 22-year-old Marine who presented to the emergency department, after a martial arts exercise, with transient weakness and numbness in all extremities. Computed tomography cervical spine radiographs revealed os odontoideum. Lateral flexion–extension radiographs identified atlanto-axillary instability. This abnormality is rare and can be career ending for military members who do not undergo surgical fusion. [West J Emerg Med. 2011;12(4:520–522.

  9. Analysis of risk factors associated with precancerous lesion of gastric cancer in patients from eastern China: A comparative study

    OpenAIRE

    Yanmin Wu; Yaofu Fan; Yangyang Jiang; Yao Wang; Hao Liu; Muxin Wei

    2013-01-01

    Purpose: To investigate the association between various risk factors and precancerous lesion of gastric cancer (PLGC) in patients from eastern China. Materials and Methods: 501 cases of PLGC and 523 cases of superficial gastritis were included. A comparative study of the relation between different risk factors and PGLC was performed. Results: Statistical differences were noted in a series of indexes including Helicobacter pylori (HP) infection, family history of esophageal cancer (EC), ...

  10. Severe cervical glandular cell lesions and severe cervical combined lesions - Predictive value of the Papanicolaou smear

    NARCIS (Netherlands)

    Erp, AJMV; Smedts, FMM; Vooijs, GP

    2004-01-01

    BACKGROUND. The purpose of the current study was to determine the accuracy of routinely screened cervical smears to predict a glandular cell lesion in histologically confirmed cases of cervical adenocarcinoma in situ (AIS), invasive adenocarcinoma (ADCA), adenosquamous carcinoma (ADSQCA), and severe

  11. Immunohistochemical expression of MIB-1 and PCNA in precancerous and cancerous lesions of uterine cervix

    Directory of Open Access Journals (Sweden)

    Madhu Mati Goel

    2013-01-01

    Full Text Available Background and Objective: The present study was done to analyze the immunoexpression of diagnostic markers (MIB-1: molecular immunology borstel and PCNA: proliferating cell nuclear antigen in grading cervical intraepithelial lesion (CIN and squamous cell carcinoma (SCC in cervix. Setting and Design: Total 150 cervical biopsies were divided into four groups respectively; Group I-Normal (n = 32, Group II- CIN (n = 60, Group III- SCC (n = 44, Group IV- CA cervix (n = 14 respectively. Materials and Methods: These biopsies were stained with monoclonal antibodies by streptavidin--biotin method. Mean labeling index was calculated and grading was performed using the I--III scoring system. Statistical Analysis: Findings were correlated with age and menopausal status. Statistical analysis was done by using student sample′t′ test and analysis of variance (ANOVA by SPSS 10 package. Results: MIB-1 immunostaining was positive in 112/150 (74.6% cases and PCNA in 118 /150 (78.6% cases. Labeling indices showed linear progression from normal to CIN to SCC to cancer lesion. Few cases of low-grade CIN lesion had high proliferative index. A significant positive correlation was found between age and PCNA and MIB-1 values (P < 0.05 when comparison was made for all the cases. Conclusion: These markers may be useful in identifying low-grade CIN lesion with high proliferative index. These cases should be kept for follow up studies so that proper intervention can be taken at an early stage. This method is simple and cost effective and can easily be done in formaline-fixed paraffin embedded tissues in a clinical laboratory for grading CIN and SCC lesions in cervix.

  12. Expression changes of JNK and STAT3 in cervical precancerous lesions and cervical cancer tissues and its clinical value%宫颈上皮内瘤样病变及宫颈癌组织中JNK、STAT3蛋白的表达变化及意义

    Institute of Scientific and Technical Information of China (English)

    冯姝; 瞿全新

    2013-01-01

    Objective To observe the expression changes of c-Jun N-terminal kinase(JNK) and signal transducer and activator of transcription 3( STAT3) in cervical intraepithelial neoplasia( CIN) and cervical cancer( CC) tissues and to explore the significance. Methods The JNK and STAT3 protein expression was detected by immunohistochemical SABC method in 10 cases of normal cervical epithelium( NCE) , 105 cases of cervical intraepithelial neoplasia( CIN) and 66 cases of cervical cancer( CC) , and then the relationship of JNK and STAT3 with clinicopathological parameters of cervical cancer was analyzed. Results The positive expression rate of JNK protein in NCE, CIN and CC tissue was 10.0% , 45. 7% and 75.8% respectively. The differences above all have statistic significance ( P <0. 05). Meanwhile, the positive expression rate of STAT3 protein in NCE , CIN and CC tissue was 10% ,48.6% and 83.3% respectively. The differences above all had statistic significance(P <0. 05). The JNK and STAT3 expression was not related to age, clinical stage and lymph node metastasis, but related to histological grade of CC. The expression higher in poorly differentiated carcinoma than in well-moderately differentiated one( P < 0.005, x2 = 12. 08,12.61). Conclusions The expression of JNK and STAT3 protein in cervical intraepithelial neoplasia and cervical cancer increase and correlate with the carcinogenesis, development and differentiation of cervical cancer. JNK and STAT3 may be promising candidates for predicting prognosis in CC and have important implications for individual treatment.%目的 观察宫颈上皮内瘤样病变(CIN)及宫颈癌(CC)组织中c-Jun氨基末端激酶(JNK)和信号转导及转录活化因子3(STAT3)的表达变化,并探讨其意义.方法 采用免疫组化链霉亲和素—生物素—过氧化物酶(SABC)法检测10例正常宫颈组织(NCE)及105例CIN、66例宫颈癌(CC)组织中JNK和STAT3蛋白的表达情况,并分析其阳性表达率与CC临

  13. Positive diagnostic values and histological detection ratios from the Rotterdam cervical cancer screening programme

    NARCIS (Netherlands)

    F.A. Kreuger; H. Beerman (Henk); H.G. Nijs (Huub); M. van Ballegooijen (Marjolein)

    1998-01-01

    textabstractBACKGROUND: In organized screening programmes for cervical cancer, pre-cancerous lesions are detected by cervical smears. However, during follow-up after a positive smear these pre-cancerous lesions are not always found. The purpose of the study is to analys

  14. Diaphragm paralysis from cervical disc lesions.

    Science.gov (United States)

    Cloward, R B

    1988-01-01

    An opera singer, who "made her living with her diaphragm", developed a post-traumatic unilateral radiculopathy due to cervical disc lesions, C3 to C6. During one year of severe neck and left arm pain she gradually lost the ability to sing difficult operatic passages which brought an end to her music career. Following a three level anterior cervical decompression and fusion, the neck and arm pain was immediately relieved. One week later her voice and singing ability returned to its full strength and power permitting her to resume her activities as a vocalist. The diagnosis of paresis of the left hemi-diaphragm as part of the cervical disc syndrome was implied by postoperative retrospective inference.

  15. HPV阴性的宫颈癌和癌前病变中p14ARF蛋白表达及其临床意义%Expression and clinical significance of p14ARF protein in HPV - negative patients with cervical cancer and cervical precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    尹香花; 苏悦; 隋玉梅; 成艳

    2011-01-01

    Objective:To explore the relationship between pl4ARF protein, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and early diagnosis, invasion and metastasis of cervical cancer by detecting their expression levels in cervical canc-er and cervical intraepithelial neoplasia (CIN) of human papillomavirus ( HPV ) - negative patients. Methods: 30 HPV - negative patients with chronic cervicitis, 35 HPV - negative patients with CIN and 35 HPV - negative patients with cervical invasive cancer were selected as study ob-jects by PCR. Immunohistochemical SP staining was used to detect the expression level of pl4ARF protein, clinicopathological characteristics and the correlation with the depth of invasion and lymph node metastasis. Results :P14ARF protein specifically expressed in the nuclei and cyto-plasm of CIN and cervical cancer cells, and it never expressed in normal squamous epithelium and glandular epithelium, there was no correla-tion between P14ARF protein expression and clinical stages, while it was related to the degree of differentiation and lymph node metastasis. Conclusion: pl4ARF protein detection can be used as an index for early diagnosis of cervical cancer among HPV - negative patients, which also can be used as an index to predict the potential of invasion and metastasis of cervical cancer and clinical prognosis.%目的:通过检测人乳头状瘤病毒(HPV)感染阴性的官颈癌和宫颈上皮内瘤变中p14ARF和基质金属蛋白酶MMPs及其抑制物TIMPs的表达,探讨其与官颈癌早期诊断、侵袭转移的关系.方法:以PCR方法筛查HPV阴性的慢性官颈炎30例、官颈上皮内瘤变(CIN) 35例及官颈浸润癌35例作为研究对象,采用SP染色法检测p14ARF蛋白在其中的表达,分析p14ARF蛋白在官颈癌组织中的表达、临床病理特征及其与浸润深度、淋巴结转移的相关性.结果:p14ARF蛋白特异性表达在HPV阴性的CIN病变、宫颈癌细胞核及胞质中,在

  16. Qualitative and Quantitative Studies of Polygene Proteins Expression in Esophageal Precancerous Lesions and Esophageal Carcinoma

    Institute of Scientific and Technical Information of China (English)

    LI Chao-xia; WU Ming-yao; KUANG Li-ping

    2007-01-01

    Objective: To examine the expressions of MDM2, P53 and P27 proteins in chronic esophagitis, para-cancer mucosa and esophageal carcinoma. Methods: Immunohistochemistry was used to detect the expressions of MDM2, P53 and P27 proteins in forty-seven patients suffering from chronic esophagitis and eighty-five cases of esophageal carcinoma and corresponding para-cancer mucosa. Flow cytometry((FCM) was applied to detect the quantities of these proteins expressed in fresh tissues of 48 cases of esophageal cancer and their para-cancer tissues and 24 cases of relative normal mucosa at the surface of cutting edge. Results: Immunohistochemistry results showed that the expressions of the three studied proteins were very similar in the epithelia of chronic esophagitis and para-cancer mucosa (P>0.05). Both the qualitative and quantitative studies displayed that the P53 protein had no expression and its accumulations would appear only in the early stages of esophagus canceration while the MDM2 and P27 proteins had different degrees of expressions in cases of normal esophageal mucosa. MDM2 protein markedly increased in the advanced stages of esophageal canceration. A quantitative study showed that the expression of P27 protein had a linearity of decreasing tendency (F=9.132,P=0.002) in the course of esophageal canceration. Conclusion: Chronic esophagitis may be a precancerous lesion. Owing to the changes of the P53 and P27 proteins, we can also conclude that these occur in the early stages of esophagus oncogenesis, however the changes of MDM2 expression may occur in the advanced stage of esophageal canceration.

  17. Clinical Value of TCT, HPV Test, Colposcopy Biopsy and LEEP in Cervical Cancer and Precancerous Lesions%TCT、HPV检测、阴道镜活检及LEEP在宫颈癌及癌前病变中的临床价值

    Institute of Scientific and Technical Information of China (English)

    谷冀晖; 刘毅; 冯文; 戚挺; 王颖; 张阳

    2016-01-01

    Objective To investigate the clinical significance of TCT, HPV, colposcopic cervical biopsy and LEEP in cervical intraepithelial neoplasia.Methods From September 2011 to January 2015, 2 146 patients were treated in the First People's Hospital of Lianyungang. TCT was performed routinely before operation. 338 patients underwent colposcopic cervical biopsy, which suggested that cervical intraepithelial neoplasia,the line ring electrosurgical treatment, specimen pathology.Results (1) 2 146 cases of TCT examination, 308 cases of abnormal accounted for 14.35%, of which 97 cases of ASCUS accounted for 31.49%, 153 cases of LSIL accounted for 49.68%, 49 cases of HSIL accounted for 15.91%, 9 cases of SCC accounted for 2.92%.(2) Comparing the postoperative pathological results of TCT and LEEP, CIN and cervical cancer detection rate was 83.12% in the TCT-positive report. The coincidence rate of LSIL and HSIL was 62.75% and 85.71%, respectively. The difference between the coincidence rate of LSIL and HSIL was signiifcant (χ2 = 5.82,P<0.05). (3) Results of the HPV detection combined with TCT test: the detection rate of CIN was 93.53%, in which CINⅡ~Ⅲ detection rate was 50.86%. (4) The coincidence rate of histopathological and colposcopic biopsy was 66.56% in 338 cases.ConclusionTCT is the main method of cervical disease screening, HPV positive screening rate and pathological grade was positively correlated, TCT and HPV combined detection can improve the detection rate of cervical lesions, early detection of cervical cancer has a guiding signiifcance, cervical biopsy is not easy to check out cervical deep tissue and cervical lesions, LEEP surgery can effectively diagnose cervical lesions, while a therapeutic effect.%目的:探讨TCT检查、HPV检测、阴道镜宫颈活检、LEEP术在子宫颈上皮内瘤病变中临床意义。方法2011年9月~2015年1月在连云港市第一人民医院就诊的患者共2146例,术前常规行TCT检查,338例患者在阴道镜

  18. Application of magnifying narrow-band imaging endoscopy for diagnosis of early gastric cancer and precancerous lesion

    Directory of Open Access Journals (Sweden)

    Zhang Jing

    2011-12-01

    Full Text Available Abstract Background Gastric carcinoma is the second commonest cause of cancer deaths worldwide. Early detection and diagnosis of gastric cancer in the stomach is important for improving the prognosis of gastric cancer. This retrospective study was designed to investigate the value of magnifying narrow-band imaging (NBI in the diagnosis of precancerous lesions and early gastric cancer. Methods This study included 122 patients who were diagnosed with early gastric cancer or precancerous gastric lesions by endoscopy. The patients underwent an examination with conventional endoscopy, magnifying NBI, and magnifying chromoendoscopy. Images resolution was evaluated, and the morphology, pit patterns and blood capillary forms of lesions were analyzed. The presence of gastric carcinoma and high grade intraepithelial neoplasia in the biopsy samples was considered as a positive pathological result, which is used to assess accuracy of endoscopic diagnosis. Results For image resolution, magnifying NBI and magnifying chromoendoscopy were significantly superior to magnifying conventional endoscopy in morphology, pit pattern and blood capillary form (P 1, and VI type of gastric pit pattern were detected in 14 cases, 43 cases, and 17 cases in patients with high grade intraepithelial neoplasia, respectively. V1 and VI type of gastric pit pattern were detected in 9 cases and 39 cases in patients with early gastric cancer, respectively. The presence of irregular minute vessels and variation in the caliber of vessels was found in 109 cases. The accuracy, sensitivity, specificity, false positive rate and false negative rate for diagnosis of early gastric cancer and precancerous gastric lesions were 68.9%, 95.1%, 63.1%, 24.5%, and 32.4% for conventional endoscopy, 93.6%, 92.7%, 94.5%, 5.7%, and 6.9% for magnifying NBI, and 91.3%, 88.6%, 93.2%, 13.2%, and 21.48% for magnifying chromoendoscopy, respectively. Conclusions This study demonstrates that magnifying NBI is

  19. Analysis of the depolarizing properties of normal and adenomatous polyps in colon mucosa for the early diagnosis of precancerous lesions

    Science.gov (United States)

    Ortega-Quijano, Noé; Fanjul-Vélez, Félix; de Cos-Pérez, Jesús; Arce-Diego, José Luis

    2011-09-01

    Optical characterization of biological tissues by means of polarimetric techniques is an area of growing interest. Polarized light can be used for malignant neoplasms detection. To our knowledge, few studies have so far focused on lesions that are prone to result in cancer. In this work we present a polarimetric study of depolarization in prepathological tissues. Specifically, we will focus on premalignant lesions in human colon due to their clinical relevance. Colonic adenoma, the potential precursor of malignant adenocarcinoma, provokes significant structural modifications in colon mucosa that affect light depolarization. The depolarizing properties of normal and adenomatous polyps mucosa are compared. The average linear degree of polarization is shown to present a strong dependence with the precancerous state of the colonic tissue. This method has the potential to enable an early diagnosis of colon cancer.

  20. EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR AND HUMAN PAPILLOMAVIRUS (HPV L1 CAPSID PROTEIN IN CERVICAL SQUAMOUS INTRAEPITHELIAL LESIONS

    Directory of Open Access Journals (Sweden)

    Balan Raluca

    2010-09-01

    Full Text Available We analyzed the immunohistochemical pattern of epidermal growth factor receptor (EGFR in cervical squamous intraepithelial lesions (SILs in correlation with L1 HPV capsid protein, in order to determine the relationship between EGFR expression and the infection status of human papillomavirus (HPV. The study included 40 cases, 24 LSIL (low grade SIL (CIN1, cervical intraepithelial neoplasia and 16 HSIL (high grade SIL (6 cases of CIN2 and 10 cases of CIN3. The immunoexpression of L1 HPV protein was assessed on conventional cervico-vaginal smears and EGFR was immunohistochemically evaluated on the corresponding cervical biopsies. The HPV L1 capsid protein was expressed in 45.83% of LSIL and 25% of HSIL. EGFR was overexpressed in 62,4% of HSIL (58,4% CIN2 and 41,6% CIN3 and 37,6% LSIL. The immunoexpression of L1 HPV has clinical application in the progression assessment of the cervical precancerous lesions without a correlation to the grade of the cervical SIL. EGFR is expressed by all proliferating squamous epithelial cells, thus corresponding with the grade of SIL. The evaluation of EGFR status, correlated with L1 HPV protein expression, can provide useful data of progression risk of cervical squamous intraepithelial lesions

  1. Comparison Between Pap Smear and Via As Screening For Cervical Lesions

    Directory of Open Access Journals (Sweden)

    Harshini V 1, Amritha Bhandary 2, Suchithra Thunga 3

    2013-09-01

    Full Text Available Introduction: An important reason for higher cervical cancer incidence in developing countries is lack of effectivescreening programs like pap smear, aimed at detecting precancerous conditions before they progress to invasivecancer. The potential difficulties in implementing cervical cytology based screening in low-resource settings haveprompted the investigation of accuracy of alternative low technology tests such as Visual inspection with acetic acidapplication [VIA], Visual inspection with acetic acid application with magnification [VIAM], visual inspection onLugol’s Iodine application [VILI] in early detection of cervical neoplasia .In our study we compared pap smear withVIA to study the accurarcy of VIA as it is simpler and easier technique to be used as screening in low resourcesettings. Aim: This is a hospital based descriptive, prospective study to evaluate validity of pap smear and VIAtechniques as screening tests in identifying cervical lesions. Materials And Methods: After general and systemicexamination as a routine,visual local pelvic examination including visualisation of cervix and vagina per speculumand the findings are documented in the proforma .Then VIA and pap smear are done in that order, if any of thesetests are positive then cervical biopsy will be taken and further advise to the subject is given. Results: A total of 313women were involved in the study. The sensitivity of pap smear is 54.5% specificity is 98.9% while that of VIA95.4% and 97.9%respectively. We found that VIA accuracy was comparatively more than that of pap smear.Conclusion: In low resource settings, usefulness of VIA is more than that of pap smear. We suggest to perform VIAin all the women inspite of having pap smear facility to improve detection rate of cervical lesions and provide betterpatient councelling and treatment.

  2. Expression of gastric cancer—associated MG7 antigen in gastric cancer,precancerous lesions and H.pylori—associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Dong-LiGuo; MingDong; LanWang; Li-PingSun; YuanYuan

    2002-01-01

    AIM:To investigate the relationship between the antigen MG7 antigen expression and gastric cancer as well as precancerous condition;to study the relationship between the MG7 antigen expression and H.pyiori infection in benign gastric lesions in order to find out the effect of H.pylori infection on the process of gastric cancer development.

  3. Effects of San Qi on Gastric Secretion and Protective Factors of Gastric Mucosa in the Rat with Precancerous Lesion of Stomach

    Institute of Scientific and Technical Information of China (English)

    石雪迎; 赵凤志; 戴欣; 董秀云; 方杰; 杨会敏

    2003-01-01

    @@ In the model rat with precancerous lesion of stomach induced by the combined method of insertion of a spring into the pylorus and high salt hot paste, effects of San Qi (三七 Radix Notoginseng) on gastric secretion and protective factors of stomach were investigated.

  4. Human Papillomavirus types distribution among women with cervical preneoplastic, lesions and cancer in Luanda, Angola

    Science.gov (United States)

    Damião, Paciência de Almeida; Oliveira-Silva, Michelle; Moreira, Miguel Ângelo; Poliakova, Natalia; de Lima, Maria Emilia RT; Chiovo, José; Nicol, Alcina Frederica

    2016-01-01

    Introduction Cervical cancer is the leading cause of cancer deaths among females in Angola and human papillomavirus (HPV) is the main risk factor for the development of pre-cancerous squamous intraepithelial lesions. The diversity and frequency of HPV types in Angola has yet to be reported. Aim To determine the frequency of HPV among women with squamous intraepithelial lesions from women in Luanda, Angola. Methods Study participants included women diagnosed with cytological abnormalities that voluntarily provided Pap smears (n = 64). Genomic DNA was extracted from the samples for use as templates in the PCR amplification of HPV sequences. PCR products were sequenced to determine HPV type. Results HPV DNA was detected in 71.9% (46/64) in the samples. A higher diversity of HPV types was found in the cytological lesions, such as ASCUS and LSIL (HPV16, 6, 18, 31, 58, 66, 70 and 82, in order of frequency) than that detected for HSIL and SSC (HPV16, 18, 6 and 33). The most prevalent HPV type were: HPV16, HPV6 and HPV18. Conclusion This is the first report on HPV type diversity and frequency in woman of Angola. The results suggest that large-scale studies across Africa would improve our understanding of interrelationship between HPV infections and cervical cancer. More directly, the identification of the HPV types most prevalent suggests that women in Angola would benefit from currently available HPV vaccines. PMID:28154623

  5. Diversity of HPV types in cancerous and pre-cancerous penile lesions of South African men: implications for future HPV vaccination strategies.

    Science.gov (United States)

    Lebelo, Ramokone L; Boulet, Gaëlle; Nkosi, Cornelius M; Bida, Mechack N; Bogers, John-Paul; Mphahlele, M Jeffrey

    2014-02-01

    This study reports the detection of HPV types from cancerous and pre-cancerous penile lesions that were diagnosed histologically. Sixty-six (22 pre-cancerous and 44 cancerous lesions) tissue biopsies, received between 2004 and 2011 by the Anatomical Pathology Department at Dr. George Mukhari Hospital were selected for this study. Total DNA was extracted and genotyped using type specific real-time quantitative polymerase chain reaction (qPCR) for 18 HPV types. Of 66 samples, only 51 were included in the analysis. Overall, HPV 11 (50.9%) and HPV 16 (49.1%) showed almost similar incidence in the study patients. In pre-cancerous lesions, HPV 11 was more frequent (80.0%), followed by HPV 31 and HPV 16 at 25.0% each and other HPV types included 35 (15.0%), 59 (15.0%), 53 (10.0%), 33 (10.0%), 18 (5.0%), 51 (5.0%), 52 (5.0%), 56 (5.0%), and 67 (5.0%). For cancerous lesions, HPV 16 was the most detected (62.9%), followed by HPV 11 (34.3%), and other HPV types included 18 (11.4%), 33 (5.7%), 39 (5.7%), 45 (5.7%), 66 (5.7%), 52 (2.9%), 58 (2.9%), 6 (2.9%), and 67 (2.9%). Several lesions demonstrated multiple HPV infections, ranging from two to six different types in one lesion. The study showed high diversity of HPV types in cancerous and pre-cancerous lesions of South African males with the most frequent being HPV types 11 and 16. The data suggest that boys could directly benefit from vaccination as they are exposed to variety of HPV types as early as 10 years of age in Africa.

  6. Epithelial abnormalities and precancerous lesions of anterior urethra in patients with penile carcinoma: a report of 89 cases.

    Science.gov (United States)

    Velazquez, Elsa F; Soskin, Ana; Bock, Adelaida; Codas, Ricardo; Cai, Guoping; Barreto, Jose E; Cubilla, Antonio L

    2005-07-01

    Urethral and penile tissues and their neoplasms are considered anatomically and pathogenetically different. Since we observed urethral dysplastic lesions and some similarities between noninvasive and invasive lesions of the anterior urethra and glans, we designed this study to document epithelial urethral abnormalities in patients with penile squamous cell carcinoma. We examined urethral epithelia from 170 penectomies with invasive squamous cell carcinoma finding a variety of primary epithelial abnormalities in 89 cases (52%) and secondary invasion of penile carcinoma to urethra in 42 cases (25%). Patients' average age was 68 years. Primary tumors measured 4 cm in average diameter and the majority were squamous cell carcinoma of the usual (67%) or verrucous type (15%). Primary epithelial abnormalities found were squamous intraepithelial lesions, metaplasias and microglandular hyperplasias. Urethral squamous intraepithelial lesions of high grade was found in six patients and of low grade in eight cases. Squamous metaplasia, seen in 69 cases, was the most frequent finding. Metaplasias were classified as nonkeratinizing and keratinizing. Nonkeratinizing metaplasias (57 cases) were variegated in morphology: simplex (26 cases), hyperplastic (12 cases), clear cell (11 cases) and spindle (8 cases). Keratinizing metaplasias (12 cases) showed hyperkeratosis and were more frequently associated with verrucous than nonverrucous penile squamous cell carcinoma. Microglandular hyperplasia was present in eight cases. Lichen sclerosus was associated with simplex squamous metaplasia in four cases. Despite the large size of the primary tumors, direct urethral invasion by penile carcinoma was present in only 25% of the cases. The presence of precancerous lesions in urethra of patients with penile carcinoma indicates urethral participation in the pathogenesis of penile cancer. Simplex squamous metaplasia is a common finding probably related to chronic inflammation. Keratinizing and

  7. The Value of DNA Ploidy Technology in Cervical Cancer and Precancerous%DNA倍体技术在宫颈癌和癌前病变筛查中的价值探讨

    Institute of Scientific and Technical Information of China (English)

    刘晓艳

    2014-01-01

    Objective:To investigate the value of DNA ploidy technology in cervical cancer and precancerous. Method:1538 cases were be screened,the first pathological biopsy performed after DNA ploidy analysis,then differences between the DNA ploidy and biopsy examination were compared.Result:The samples were drawn from 1538 cases,DNA ploidy negative analysis was 1425 cases,negative rate was 92.7%,the highest positive rate was over 51 years old and followed by 31 to 40 years old,pathological diagnosis of cervical cancer or precancerous lesions was 55 cases,DNA ploidy analysis missed the presence of two cases,the diagnosis rate was 96.4%.Conclusion:DNA ploidy analysis and screening for early diagnosis of cervical cancer and precancerous lesions,the diagnostic accuracy rate is high,it can easily obtain and check the simple non-invasive,so it is a more reliable screening method.%目的:探讨DNA倍体技术在宫颈癌和癌前病变筛查中的价值。方法:选取1538例待筛查者,先进行DNA倍体分析之后进行病理活检检查,比较DNA倍体分析结果与病理活检检查的差异。结果:本组共取材1538例样本,DNA倍体分析阴性者1425例,阴性率92.7%,51岁及以上阳性率最高,其次为31~40岁者,病理检查确诊宫颈癌或者癌前病变者55例,DNA倍体分析存在2例漏诊,其诊断率达到96.4%。结论:DNA倍体分析对于宫颈癌及其癌前病变的早期诊断及筛查,诊断符合率高,取材方便,检查简单无创,是一种较为可靠的普查方法。

  8. Human Papillomavirus Genotyping and p16(INK4a) Expression in Cervical Lesions: A Combined Test to Avoid Cervical Cancer Progression.

    Science.gov (United States)

    Zouheir, Yassine; Fechtali, Taoufiq; Elgnaoui, Nadia

    2016-06-01

    Cervical cancer is a major public health problem in Morocco. The cervical cancer has a long precancerous period that provides an opportunity for the screening and treatment. Improving screening tests is a priority goal for the early diagnosis of cervical cancer. This study was conducted to evaluate the combination of p16(INK4a) protein expression, human papillomavirus (HPV) typing, and histopathology for the identification of cervical lesions with high risk to progress to cervical cancer among Moroccan women. A total of 96 cervical biopsies were included in this study. Signal amplification in situ hybridization with biotinylated probes was used to detect HPV. Immunohistochemistry was used to evaluate the expression of p16(INK4a) protein. HPV DNA was detected in 74.0% of the biopsies (71/96). Of the seventy-one positive HPV cases, we detected 67.6% (48/71) of high risk (HR)-HPV (HPV 16 and 18), 24% of low risk-HPV (HPV 6 and 11), 1.4% intermediate risk-HPV (HPV 31, 33, and 35), and 7% coinfections (HPV 6/11 and 16/18). Overexpression of p16(INK4a) protein was observed in 72.9% (70/96) of the biopsies. In addition, p16(INK4a) protein detection was closely correlated with recovery of HR HPV. Our result showed that p16(INK4a) expression level is correlated with HR-HPV status.

  9. DNA ploidy analysis in oral cancer and precancerous lesions%DNA倍体分析在口腔癌及癌前病变中的应用

    Institute of Scientific and Technical Information of China (English)

    宁毅; 范永锋; 孙睿

    2016-01-01

    Objective To evaluate and compare the value of exfoliated cell DNA ploidy analysis and toluidine blue staining in the diagnosis of precancerous lesions and oral cancer. Methods A total of 220 outpatients and inpa-tients hospitalized in the Department of Oral Surgery, Shanxi Provincial People′s Hospital, were included in the study. All patients were grouped and examined by DNA ploidy analysis and toluidine blue staining. Results DNA ploidy analysis showed high specificity and sensitivity for precancerous lesions and malignant lesions. Toluidine blue staining showed moderate sensitivity and low specificity for precancerous lesions and malignant lesions. Conclusion For the diagnosis of oral cancer and precancerous lesions, exfoliated cells-DNA ploidy analysis is better than toluidine blue staining in sensitivity and specificity.%目的 评价和比较脱落细胞DNA倍体分析与甲苯胺蓝染色两种方法对诊断癌前病变和口腔癌的应用价值.方法 收集山西省人民医院口腔外科门诊及住院患者220例,分组应用DNA倍体分析和甲苯胺蓝染色两种检查方法检测.结果 DNA倍体分析对癌前病变和恶性病灶都显示出高特异性和灵敏度;而甲苯胺蓝对癌前病变和恶性病灶染色结果显示出中等灵敏度和低特异性.结论 对于口腔癌及癌前病变的诊断,脱落细胞DNA倍体分析在灵敏度和特异度方面都优于甲苯胺蓝染色.

  10. The Prevalence of Human Papilloma Virus(HPV in Malignant Cervical Lesion, Using Multiplex PCR

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    M. R. Keyhkhaee

    2006-07-01

    Full Text Available Background: Cervical cancer is the second leading cause of cancer death among women. In this cancer, the effects of prevention, early diagnosis and treatment more than other cancers decrease the mortality rate. In 1970 human papilloma virus (HPV was introduction as major etiologic factor of cervical cancer. Different studies throughout the world revealed strong correlation between HPV and cancerous & precancerous changes in epithelial cells. Since cell culture and serological methods can not recognize the virus and its subtypes, the importance of the molecular methods including polymerase chain reaction (PCR in early and definite diagnosis of virus is obvious. Methods: In this study, after patient selection using the related protocol and completion of the questionnaires, 100 samples from cancer lesions of cervix selected. Then DNA extraction from paraffin blocks performed using standard method. Multiplex PCR with two pairs of primer (one as internal control performed and the PCR product run on 8% polyacrylamid gel. Results: The results showed that 73% of the tissues were infected by HPV. Conclusion: This finding confirm the previous results based of correlation between HPV,and cervical cancer.

  11. Serum vitamin D levels of patients with oral squamous cell carcinoma (OSCC) and expression of vitamin D receptor in oral precancerous lesions and OSCC

    Science.gov (United States)

    Cetindis, Marcel; Biegner, Thorsten; Lehman, Max; Munz, Adelheid; Teriete, Peter; Reinert, Siegmar

    2015-01-01

    Background: Resistance to programmed cell death (apoptosis) is a crucial factor for the carcinogenesis of oral squamous cell carcinoma (OSCC). Vitamin D (calcitriol) may overcome apoptosis resistance in tumor cells of OSCC. Vitamin D receptor (VDR) expression in oral precancerous lesions of OSCC has not been analyzed and serum vitamin D level seems to be a predictor of cancer development. Material and Methods: Expression of VDR was analyzed in normal oral mucosa (n=5), oral precursor lesions (simple hyperplasia, n=11; squamous intraepithelial neoplasia, SIN I-III, n=35), and OSCC specimen (n=42) by immunohistochemistry (IHC). Moreover, serum vitamin D levels were measured by 25(OH)D3 (calcidiol) in patients with OSCC (n=42) and correlated with IHC results. Results: Expression of VDR was significantly increased in precancerous and OSCC compared with normal tissue. Compared with SIN I-III lesions VDR expression significantly decreased in OSCC. Severe vitamin D deficiency was detected in our OSCC patient cohort but there was no significant correlation analyzed between serum vitamin D levels and corresponding immunohistochemically detected VDR expression in OSCC. Conclusions: Our survey provides the first evidence of VDR expression in precancerous lesions of OSCC. Apoptosis induction of VDR+ cells in oral precancerous lesions and OSCC by natural vitamin D or synthetic vitamin D compounds could be useful for chemoprevention. Moreover, systemically and/or locally applied, these compounds may act as sensitizers for apoptosis mediated by radio-, and chemotherapy treatment in OSCC. Key words:Oral cancer, oral precancer, lichen planus, leukoplakia, apoptosis, serum 25(OH)D3, vitamin D receptor, chemoprevention, multistep carcinogenesis. PMID:25662556

  12. Expression of HIF-1α in breast cancer and precancerous lesions and the relationship to clinicopathological features

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    Yun’ai Liang; Zengxin Li; Gangping Wang

    2014-01-01

    Objective: The aim of this study was to observe the expressions and clinical significance of HIF-1α in breast cancer and precancerous lesions, and analyze the relationship between the expressions and clinicopathological features in breast cancer. Methods: We analyzed the HIF-1α expression in 128 cases of invasive ductal carcinomas, 146 precancerous lesions patients including 89 cases of ductal carcinoma in situ and 57 cases of atypical ductal hyperplasia. 53 cases of usual ductal hyperplasia breast tissues were selected as a control group. The specimens were evaluated for HIF-1α, estrogen re-ceptor (ER) & progesterone receptor (PR), epidermal growth factor receptor type 2 (HER2/neu) and Ki-67. Immunoreactivity was semi-quantitatively evaluated in at least 1000 cells examined under the microscope at 40 × magnification and recorded as the percentage of positive tumor cells over the total number of cells examined in the same area. The percentage scores were subsequently categorized. The express of HIF-1α and their relationship with multiple biological parameters including ER& PR, HER2/neu and Ki-67, the biomarkers levels of CA153, CA125 TSGF, and CEA in blood serum and nipple discharge, histological grade, region lymph node metastasis, distant metastasis and recurrence on files were also assessed. Results:Compared with usual ductal hyperplasia, the positive expression rate of HIF-1α in atypical ductal hyperplasia, ductal carci-noma in situ and invasive ductal carcinomas group was significantly increased (P 14% groups, histological grade (I + II) and grade III invasive ductal carcinomas groups, with lymph node metastasis, distant metastasis and recurrence groups (P50 years), tumor diameter (≤ 2 cm vs > 2 cm; P > 0.05). The nipple discharge and serum levels of CA153, TSGF, CA125 and CEA in invasive ductal carcinomas HIF-1α positive patients were significantly higher than those in the negative patients (P <0.05). Conclusion: In breast cancer, HIF-1α expression

  13. Diagnostic tests for the detection of human papillomavirus-associated cervical lesions.

    Science.gov (United States)

    Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-01-01

    Current diagnostic approaches for primary cervical cancer screening, work-up of equivocal or positive screening results or follow- up after treatment of precancerous lesions primarily rely on the morphologic interpretation of squamous epithelial cells (Pap cytology), in some setting accompanied by the detection of human papillomavirus DNA and have largely contributed to remarkable reduction of disease incidence in countries with implemented screening programs. However, these approaches are limited by a poor sensitivity and reproducibility of Pap cytology and low specificity for high grade cervical intraepithelial neoplasia of HPV DNA detection assays. Early detection might be improved by complementing or even replacing these tests by markers which are more directly related to molecular events triggering HPV-induced carcinogenesis and thereby might deliver more accurate diagnostic performance. The delineation of molecular changes which occur during different stages of HPV infections and the identification of changes which induce neoplastic alterations allow for the detection of markers that specifically highlight the transforming stage of the infection where viral oncogenes are overexpressed and therefore allow for a more specific diagnosis of lesions that require treatment. The evaluation of such markers in clinical studies revealed that some indeed show an improved diagnostic performance compared to Pap cytology or HPV DNA tests only.

  14. Associated factors with cervical pre-malignant lesions among the married fisher women community at Sadras, Tamil Nadu

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    Sornam Ganesan

    2015-01-01

    Full Text Available Objective: To identify the associated factors of cervical pre-malignant lesions among the married fisher women residing in the coastal areas of Sadras, Tamil Nadu. Methods: The study was conducted in five fishermen communities under Sadras, a coastal area in Tamil Nadu, India. Two hundred and fifty married fisher women residing in the area. Quantitative descriptive approach with a cross-sectional study design was used. Data were collected using a structured interview schedule for identifying the associated factors and Pap smear test was performed for identifying the pre-malignant cervical lesions among the married fisher women. Data were analyzed using descriptive and inferential statistics. Results: Among 250 women, about six (2.4% of them presented with pre-cancerous lesions such as atypical squamous cell of undifferentiated significance (ASCUS - five (2% and mild dysplasia one (0.4%. Majority of the women, about 178 (71.2% women, had abnormal cervical findings. Statistical analysis showed a significant association of risk factors such as advanced age, lack of education, low socioeconomic status, using tobacco, multiparity, premarital sex, extramarital relationship, using cloth as sanitary napkin, etc. Conclusion: The study findings clearly show the increased vulnerable state of the fisher women for acquiring cervical cancer as they had many risk factors contributing to the same.

  15. Expression of NF-κB and IL-6 in oral precancerous and cancerous lesions: An immunohistochemical study

    Science.gov (United States)

    Nikitakis, Nikolaos; Sfakianou, Aikaterini; Avgoustidis, Dimitrios; Sklavounou-Andrikopoulou, Alexandra

    2016-01-01

    Background The purpose of this study was to evaluate the immunohistochemical expression of NF-κB and IL-6 in oral premalignant and malignant lesions and to investigate their possible correlation with the presence of subepithelial inflammation. Material and Methods Thirty two oral premalignant lesions, clinically compatible with leukoplakia or erythroplakia, were investigated. Microscopically, 11 of them showed hyperkeratosis and acanthosis (epithelial hyperplasia) and 21 showed dysplasia of varying degrees. Nine cases of OSCC and four control cases of normal oral mucosa were also included in the study. Immunohistochemical staining with NF-κB (p65) and IL-6 was performed. IL-6 and nuclear NF-κB staining were assessed as positive or negative. For cytoplasmic localization of NF-κB, a total score combining intensity and percentage of positive epithelial cells was additionally calculated. The presence of inflammation was also recorded. Results Intensity and total scores for NF-κΒ cytoplasmic immunostaining showed a statistically significant gradual increase from normal mucosa to OSCC (p=0.012 and p=0.026 respectively). Non-statistically significant increased NF-κΒ nuclear localization was detected in dysplasias and OSCCs. Positive statistical correlation was detected between the presence of inflammation and IL-6 expression (p=0.015). No correlation between NF-κΒ and IL-6 was detected. Conclusions NF-κΒ is activated in the early stages of oral carcinogenesis. IL-6 may have an NF-κΒ-independent role, possibly through regulation of the inflammatory response. Key words:NF-κB, IL-6, immunohistochemistry, oral squamous cell carcinoma, oral precancerous lesion. PMID:26595830

  16. Utility of Papanicolaou test in diagnosis of cervical lesions: a study in a tertiary care centre of western Uttar Pradesh

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    Mithila Bisht

    2015-05-01

    Full Text Available Background: Cervical cancer is the most common cancer among females in India. It is also one of the major causes of deaths among them. Papanicolaou (Pap cytological test helps in detecting the early epithelial abnormalities in cervical cells. The morbidity and mortality due to cervical cancer has come down in countries with well-established screening programmes at national level. This study was conducted to emphasize the value of cervical screening as a tool for early detection of non-neoplastic and neoplastic lesions of cervix. Slide positivity rate was computed and clinico-pathological correlation was done. Methods: This cross-sectional study was conducted from January 2012 to December 2014 (3 years, on 2202 women aged between 20-70 years coming for a Pap smear examination in a tertiary care hospital in Bareilly, India. After staining with conventional Papanicolaou technique, all cases were classified as per Bethesda nomenclature (2001. Results: Out of 2202 cases, 85.16% slides were found to be negative for intraepithelial abnormalities and 14.84% slides were labelled as positive for epithelial lesions. 97.67% of high grade lesions were found in women more than 40 years. Smears with epithelial cell abnormality were found more common in older age groups whereas smears negative for epithelial cell abnormality were found more common in young age groups. Conclusion: Pap test is a cost effective cancer screening and is a simple method to detect various lesions of cervix, non-neoplastic as well as neoplastic. High risk screening programmes should be directed to all women >40 years. Pap test is especially useful to diagnose precancerous lesions of cervix, thereby early detection of these lesions and subsequent proper treatment can be helpful in prevention of cervical cancer. [Int J Res Med Sci 2015; 3(5.000: 1070-1076

  17. Combining proteomics, serum biomarkers and bioinformatics to discriminate between esophageal squamous cell carcinoma and pre-cancerous lesion

    Institute of Scientific and Technical Information of China (English)

    Xiao-hui ZHAI; Jie-kai YU; Chen LIN; Li-dong WANG; Shu ZHENG

    2012-01-01

    Objective: Biomarker assay is a noninvasive method for the early detection of esophageal squamous cell carcinoma (ESCC).Searching for new biomarkers with high specificity and sensitivity is very important for the early detection of ESCC.Serum surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) is a high throughput technology for identifying cancer biomarkers using drops of sera.Methods: In this study,185 serum samples were taken from ESCC patients in a high incidence area and screened by SELDI.A support vector machine (SVM) algorithm was adopted to analyze the samples.Results: The SVM patterns successfully distinguished ESCC from pre-cancerous lesions (PCLs).Also,types of PCL,including dysplasia (DYS) and basal cell hyperplasia (BCH),and healthy controls (HC) were distinguished with an accuracy of 95.2% (DYS),96.6% (BCH),and 93.8% (HC),respectively.A marker of 25.1 kDa was identified in the ESCC patterns whose peak intensity was observed to increase significantly during the development of esophageal carcinogenesis,and to decrease obviously after surgery.Conclusions: We selected five ESCC biomarkers to form a diagnostic pattern which can discriminate among the different stages of esophageal carcinogenesis.This pattern can significantly improve the detection of ESCC.

  18. Research of cervical cytology screening in diagnosis of 370,000 cases with cervical lesion in Guangdong%广东地区37万例妇女子宫颈细胞学筛查子宫颈病变的研究

    Institute of Scientific and Technical Information of China (English)

    Baowen Zheng; Congde Chen; Anxiang Wei; Han Ran; Dong Liu; Wenling Yue; Huirong Zhou; Qingyi Zhu; Juming Su

    2009-01-01

    Objective:Analysis of the results of 37 million cases by using cervical liquid-based cytology screening.discussion the situation of cervical cancer and precancerous lesions in Guangdong province.Methods:From 2002 to 2006.using liquid-based thin-layer cytology (TCT)method screening cervical lesions within 371.929 women in Guangdong.Cytological diagnosis adopt TBS(the Bethesda system)Standard(TBS improved 2001 version of the diagnostic criteria),staastic positive rate by age and region respectively.Results:371,929 cases of gynecology cervical samples in Guangdong.331,251 cases were negative,accounted for 91.23%:cytology positive cases:32,548 cases were squamous intreepithelial lesions(8.96%),486 cases were glandular epithelial lesions(0.13%):grouped according to age:30 to 40-year-old positive rate was 9.13%,40 to 50-year-old positive rate was 9.60%.the latter had higher cytology positive rate;grouped according to region:the total samples in PRD areas were 304.951 cases,accounted for 81.99%,in which 24301 cases were positive(8.14%),in 66978 cases(18.01%)of NON-PRD regions,7645 cases were positive(11.87%),there were 1858 cases had biopsy/follow-up results from 2004t02005.and the rate was 2.88%.Conclusion:The cytological positive rate of cervical cancer and its pre-cancerous lesions was 9.09%in Guangdong(of which the rate of cytological diagnosis as precancerous lesions was 3.40%),30 to 50 age group had the high incidence of cervical cancer and its precursor lesions.and also the main stage of prevention for cervical cancer.Cytology positive rate was 11.87% in economic underdevelopment Non-Pead River Delta regions.much higher than the Pearl River Delta region.

  19. Loss of heterozygosity on 10q23.3 and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Yi-Ling Li; Zhong Tian; Dong-Ying Wu; Bao-Yu Fu; Yan Xin

    2005-01-01

    AIM: To investigate the loss of heterozygosity (LOH) and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions.METHODS: Thirty cases of normal gastric mucosa, advanced and early stage gastric cancer, intestinal metaplasia, atrophic gastritis, and atypical hyperplasia were analyzed for PTEN LOH and mutations within the entire coding region of PTEN gene by PCR-SSCP denaturing PAGE gel electrophoresis,and PTEN mutation was detected by PCR-SSCP sequencing followed by silver staining.RESULTS: LOH rate found in respectively atrophic gastritis was 10% (3/30), intestinal metaplasia 10% (3/30), atypical hyperpiasia 13.3% (4/30), early stage gastric cancer 20%(6/30), and advanced stage gastric cancer 33.3% (9/30),None of the precancerous lesions and early stage gastric cancer showed PTEN mutations, but 10% (3/30) of the advanced stage gastric cancers, which were all positive for LOH, showed PTEN mutation.CONCLUSION: LOH of PTEN gene appears in precancerous lesions, and PTEN mutations are restricted to advanced gastric cancer, LOH and mutation of PTEN gene are closely related to the infiltration and metastasis of gastric cancer.

  20. Evaluation of five screening methods for an early detection of cervical cancer and its precancerous lesions in Zhejiang province%五种检测方法在宫颈癌及其前期病变筛查中的准确性评估

    Institute of Scientific and Technical Information of China (English)

    费华丽; 程易凡; 程晓东; 陈晓端; 叶枫; 吕卫国; 谢幸

    2011-01-01

    目的 比较5种现行的筛查方法在宫颈癌高发地区的应用,从而评价出最佳宫颈癌筛查方案,进而在浙江省范围内推广.方法 在浙江省丽水市某社区内计划选取1005名年龄在25~65岁、有性生活的女性进行宫颈癌筛查,筛查手段选择巴氏涂片、液基细胞学、HPV-DNA检测法、肉眼观察辅以醋酸白染色(VIA)和肉眼观察辅以Lugol's碘染色(VILI)5种.所有筛查者均接受阴道镜检查,以病理组织学结果为金标准,阴道镜检查无异常作为阴性结果.结果 巴氏涂片对宫颈上皮内高度病变的敏感度、特异度、阳性预测值、阴性预测值分别如下:25%,90%,26.7%,98.6%;液基细胞学的上述指标分别为81.3%,97.3%,35.1%,99.6%;VIA上述指标为:68.9%,82.8%,7.1%,99.3%;VILI上述指标分别为:81.3%,84.6%,9.1%,99.6%;HPV-DNA检测法上述指标为:87.5%,77.3%,6.8%,99.7%.结论 液基细胞学的诊断价值好于其余4种方法,可作为宫颈癌筛查的首选方法;肉眼观察由于其简单易行,价格低廉,可适用于经济欠发达地区的普通人群的筛查.%Objective To evaluate five screening methods of cervical cancer so as to popularize an effective screening strategy for cervical cancer in Zhejiang province. Methods A total of 1005 women aged 25 -65 years old were selected from Lishui where cervical cancer was highly prevalent. And 859 subjects were ultimately enrolled between June 2009 and December 2009. Each subject was subjected to five screening methods, including Pap smear, liquid-based cytology (LBC), human papillomavirus DNA with a second-generation hybridization assay( HC2 ), visual inspection with acetic acid (VIA) and visual inspection with Lugol's iodine (VILI). CIN (cervical intraepithelial neoplasia) 2 + on biopsy was used as the reference standard for disease positivity. Negative colposcopy was accepted as a negative outcome. Results The sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative

  1. Expression of bcl-2 oncogene in gastric precancerous lesions and its correlation with syndromes in traditional Chinese medicine

    Institute of Scientific and Technical Information of China (English)

    Ling Hu; Shao-Xian Lao; Chun-Zhi Tang

    2005-01-01

    AIM: To observe the protein and mRNA expression of bcl-2 oncogene in gastric precancerous lesions (GPL) and to analyze its correlation with syndromes in traditional Chinese medicine (TCM).METHODS: Sixty-seven patients with GPL confirmed by gastroscopy and pathology were studied, including 39 cases of moderate gastric mucosal dysplasia, 19 casesof severe gastric mucosa dysplasia, g cases of incompletecolon metaplasia. In syndrome differentiation of TCM, 17 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by qi stagnation, 21 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by stomach heat, 29 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by blood stasis. Protein and mRNA expression of bcl-2 oncogene weredetected by labeled streptavidin biotin (LSAB) immunohistochemistry and in situ hybridization respectively. RESULTS: Abnormal expression of protein and mRNA on bcl-2 oncogene was found in GPL, which increased gradually with the course of lesions. In moderate and severe gastric mucosal dysplasia and incomplete colon metaplasia, there was no difference in the expression of bcl-2 oncogene (P>0.05). In different accompanying syndromes, the expression of protein and mRNA on bcl-2 oncogene increased gradually in the following order: deficiency of both qi and yin of the spleen and stomach accompanying qi stagnation → stomach heat → blood stasis. In GPL, compared with accompanying blood stasis, there was an obvious difference in the expression of bd-2 oncogene between the syndrome of qi and yin deficiency of the spleen and stomach and accompanying stomach heat, so did accompanying qi stagnation (the level of protein: χ2 = 8.45, P<0.05; the level of mRNA: χ2 = 7.35,P<0.05).CONCLUSION: Apoptosis-associated bcl-2 oncogene is abnormally expressed in GPL, which correlates with different accompanying syndromes in TCM.

  2. Analysis of human serum from women affected by cervical lesions.

    Science.gov (United States)

    Barba de la Rosa, Ana P; Lugo-Melchor, Ofelia Y; Briones-Cerecero, Erika P; Chagolla-López, Alicia; De León-Rodríguez, Antonio; Santos, Leticia; Vázquez-Ortiz, Guelaguetza; Salcedo, Mauricio

    2008-01-01

    Cervical cancer is one of the first causes of death in Mexican women population. The plasma proteome has a wide dynamic range concentrations of different protein and their alterations reflect the physiological state of the individual's health. The aim of this study was to characterize the 2D-PAGE serum patterns from healthy women and with different levels of cervical lesions. Changes in haptoglobin, apolipoproteins, and transthyretin, when comparing the serum from healthy women and serum from patients with different levels of cervical lesion were found. The Western blot analysis showed increasing concentrations of metalloproteinases (MMP's), proteins with important biological roles in tumor development and metastasis. Protein profiles in conjunction with MS, bioinformatics, and Western blot analysis, allow us to compile information for the acquisition of results to proposed candidates biomarkers of cervical cancer among Mexican women population.

  3. Randomized Phase II Trial of Lyophilized Strawberries in Patients with Dysplastic Precancerous Lesions of the Esophagus

    Science.gov (United States)

    Chen, Tong; Yan, Fei; Qian, Jiaming; Guo, Mingzhou; Zhang, Hongbing; Tang, Xiaofei; Chen, Fang; Stoner, Gary D.; Wang, Xiaomin

    2016-01-01

    Dysplasia is a histologic precursor of esophageal squamous cell carcinoma (SCC). We previously showed that dietary freeze-dried, or lyophilized, strawberry powder inhibits N-nitrosomethylbenzylamine-induced SCC in the rat esophagus. On the basis of this observation, we conducted a randomized (noncomparative) phase II trial in China to investigate the effects of two doses of freeze-dried strawberries in patients with esophageal dysplastic lesions in a high-risk area for esophageal cancer. We randomly assigned 75 patients identified by endoscopy to have dysplastic esophageal premalignant lesions to receive freeze-dried strawberry powder at either 30 g/d (37 patients) or 60 g/d (38 patients) for six months; the powder was mixed with water and drunk. After six months, we assessed the changes in histologic grade of these lesions (primary endpoint) in a blinded fashion. The dose of 30 g/d, did not significantly affect histology or any other measured parameter. The dose of 60 g/d, however, reduced the histologic grade of dysplastic premalignant lesions in 29 (80.6%) of the 36 patients at this dose who were evaluated for histology (P < 0.0001). The strawberry powder was well tolerated, with no toxic effects or serious adverse events. Strawberries (60 g/d) also reduced protein expression levels of inducible nitric oxide synthase (iNOS) by 79.5% (P < 0.001), cyclooxygenase-2 (COX-2) by 62.9% (P < 0.001), phospho-nuclear factor kappa B (NFκB)-p65 (pNFκB-p65) by 62.6% (P < 0.001), and phospho-S6 (pS6) by 73.2% (P < 0.001). Freeze-dried strawberries (60 g/d) also significantly inhibited the Ki-67 labeling index by 37.9% (P = 0.023). Our present results indicate the potential of freeze-dried strawberry powder for preventing human esophageal cancer, supporting further clinical testing of this natural agent in this setting. PMID:22135048

  4. 分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性%linical Efficacy Analysis of Gynecatoptron and LEEP in the Treatment of Precancerous Lesions of Uterine Cervix

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2015-01-01

    Objective To analyze the effect of gynecatoptron and LEEP knife in the treatment of precancerous lesions of u-terine cervix in clinic. Methods 48 cases of patients diagnosed as cervical precancerous lesions by colposcopy biopsy pathologic examination were selected from 108 cases of vaginitis patients treated in our hospital from March 2015 to August 2015 and they were given LEEP operation by colposcopy, the clinical treatment effect was analyzed. Results by the cervix biopsy, the sensitivity ,specificity, positive predictive values, negative predictive values , misdiagnosis rate and missed diag-nosis rate of HSIL were respectively 80.83%, 91.43%, 76.38%, 92.29%, 6.9% and 10.4%. and 82.45%、90.06%、77.44%、92.54%、5.3% and 10.5% of LSIL successively, the total coincidence probability of cervix biopsy results and pathological tissue diagnosis of LEEP operation was 95.8%, the rate of conversion was 93.75% after 3 months of follow-up and 100%after 6 months of follow-up by LEEP knife treatment. Conclusion The choice of gynecatoptron and LEEP operation in the treatment of patients with precancerous lesions of uterine cervix can greatly reduce trauma and avoid severe bleeding prob-lems, the application effect of which is obvious.%目的:分析分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性。方法从2015年3—8月该院108例阴道镜患者中,整群选择48例经阴道镜活检病理检查诊断为宫颈癌前病变的入院者,给予阴道镜下LEEP手术治疗,分析其临床治疗效果。结果阴道镜的活检结果和LEEP手术的病理组织诊断的总符合概率95.8%;采用LEEP刀治疗,3个月的随访的转阴率为93.75%,6个月转阴率100%。结论阴道镜与LEEP治疗宫颈癌前病变的临床效果显著。

  5. La leucoplasia oral: Su implicación como lesión precancerosa Oral leukoplasia: A precancerous lesion

    Directory of Open Access Journals (Sweden)

    A. Martínez-Sahuquillo Márquez

    2008-02-01

    Full Text Available La leucoplasia es definida actualmente en base a un concepto esencialmente clínico. En este sentido, se propone un diagnóstico provisional de leucoplasia basado en criterios clínicos y un diagnóstico definitivo para el que se requiere la eliminación de los factores etiológicos y, ante lesiones persistentes, el examen histopatológico. La consideración de la leucoplasia como lesión precancerosa proviene esencialmente de los estudios de seguimiento en los que se constata su transformación maligna. Pese a que el diagnóstico de displasia es esencialmente subjetivo y a la existencia de algún trabajo reciente que no encuentra relación entre ésta y la evolución posterior de las leucoplasias, la presencia de displasia epitelial en el examen histológico se considera actualmente como el indicador más importante de una mayor probabilidad de transformación maligna de la leucoplasia. Entre otros factores relacionados con un peor pronóstico se consideran el que se trate de una leucoplasia idiopática, las formas clínicas no homogéneas, la localización en cara ventrolateral de la lengua y suelo de boca y un tamaño mayor de 1 centímetro.The current definition of leukoplakia is based on an essentially clinical concept. Therefore, both a provisional diagnosis of leukoplakia based on clinical criteria and a definite diagnosis for which etiological factors need to be eliminated and, should there be persistent lesions, a histological examination is required, are accepted. Follow up studies in which a malignant transformation has been observed have resulted in leukoplakia being considered as a precancerous disorder. Despite the fact that the diagnosis of dysplasia is subjective and certain recent studies have not found any association between this and the development of leukoplakia, the presence of epithelial dysplasia in the histological examination is currently considered to be the most important indicator of a higher possibility of malignant

  6. Screening cervical lesions with Fourier transform infrared spectroscopy

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The screening results were reported based on the Fourier transform infrared spectroscopy (FTIR) analysis of the samples of exfoliated cervical cells from 354 women. Their spectra can be sorted into two types based on the emerging or not of the absorption bands near 970 cm-1 and 1 170 cm-1: T1 (83.1%) type without emerging, and T2 (16.9%) type with obviously emerging. All of the samples assigned to T1 were cytologically diagnosed as normal or within normal limits (PapⅠ). 28.9% and 71.1% of samples exhibiting T2 profile, were cytologically evaluated as Pap Ⅰand abnormal respectively. 3 women in the abnormal group were diagnosed as to have cervical cells with changes associated with high grade of inflammation, cervical scar and cervical erosion. Furthermore, based on the progressive change of the relative intensities of the absorption bands, both T1 and T2 profiles can be categorized into 6 subtypes. The observed heterogeneous spectra and the progressive changes in the absorption frequencies and the relative intensities exhibit features suggestive of the progressive process of cervical lesion. The FTIR method has the potential to complement the cytological smear for large-volume screening of cervical lesions.

  7. Sialyl Lewis x expression in cervical scrapes of premalignant lesions

    Indian Academy of Sciences (India)

    Noé Velázquez-Márquez; Gerardo Santos López; Lucio Jiménez Aranda; Julio Reyes Leyva; Verónica Vallejo Ruiz

    2012-12-01

    Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumour progression and metastases. Altered expression of glycosidic antigens has been reported in cervical cancer. In cervix premalignant lesions, an increased expression of sialic acid has been reported. In the present study we determined the expression profiles of the glycosidic antigens Tn, sialyl Tn (sTn), Lewis a (Lea), sialyl Lewis a (sLea), Lewis x (Lex) and sialyl Lewis x (sLex) in cervical scrapes with cytological diagnoses of normal, low-grade squamous intraepithelial lesions (LGSIL) and high-grade squamous intraepithelial lesions (HGSIL). Cervical scrapings were collected to detect tumour antigens expressions by flow cytometry using monoclonal antibodies. Cytometry analysis of Tn, sTn, Lea and Lex did not reveal differences at the expression level among groups. The number of positive cells to sLea antigen increased in the HGSIL group with respect to the normal group (=0.0495). The number of positive cells to sLex antigen in the samples increased with respect to the grade of squamous intraepithelial lesion (SIL) ( < 0.001, Mann–Whitney U test). The intensity of expression of this antigen increased in the HGSIL samples with respect to normal samples ( < 0.0068). sLex antigen could be a candidate to be used as biomarker for the early diagnosis of cervical cancer.

  8. TTV and HPV co-infection in cervical smears of patients with cervical lesions

    Directory of Open Access Journals (Sweden)

    Tachezy Ruth

    2009-07-01

    Full Text Available Abstract Background The female lower genital tract is a gateway for pathogens entering the host through the mucous membrane. One of the prevalent human viruses is Torque teno virus (TTV. The major reported routes of TTV transmission are fecal-oral and parenteral. Furthermore, other modes of transmission, e.g. sexual contact, are suggested. To investigate the sexual route of TTV transmission, cervical smears of healthy women and those with cervical lesions were screened for the presence of TTV DNA. Methods TTV DNA was studied in cervical smears of 95 patients with cervical lesions and 55 healthy women. Paired serum samples were available from 55 and 42 women, respectively. All healthy women had normal cytology while 44 patients had histologically confirmed low-grade lesion (LGL and 51 high-grade lesion (HGL. TTV DNA was detected with primers specific for the non-coding region. In 40 paired cervical smears and serum samples, the phylogenetic group of TTV isolates was determined. The presence of HPV DNA in cervical smears was detected by means of PCR with MY09/11 primers. Results The prevalence of TTV DNA in cervical smears of healthy women was 52.7% and was comparable with that in paired serum samples (50%. Symptomatic women had significantly higher prevalence of TTV DNA in cervical smears (74.7% than healthy controls. The TTV DNA prevalence in patient serum samples was 51%. The phylogenetic groups of TTV serum isolates were concordant with those of TTV from cervical smears of the same subjects. In cervical smears, a wider variety of TTV isolates was found. The viral loads in cervical smears were 10 to 1000 times as high as in sera. The HPV-positive study subjects had significantly higher TTV DNA prevalence than HPV negatives. The prevalence of TTV was not associated with disease severity. Conclusion High prevalence of TTV in cervical smears suggests that sexual transmission is another mode of expansion of TTV infection among the population. The

  9. Serum proteomic study on early esophageal carcinoma and precancerous lesions and precancerous ;lesions in the high incidence area%高发区早期食管癌及癌前病变血清蛋白研究

    Institute of Scientific and Technical Information of China (English)

    陈仙梅; 袁丽; 郭硕; 于卫芳; 王士杰; 张立玮

    2014-01-01

    Objective This study was to screen protein biomarkers in serum of esophageal carcinoma and precancerous lesions subjects using Mass Spectrometry in high incidence area of China. Boosting decision tree was constructed to identify normal from EC and precancerous lesions, to screen significant biomarkers for early diagnosis and explore the function in progression of esophageal cancer. Methods We recruited 254 subjects, Including low-grade intraepithelial neoplasia (LGIN), high-grade intraepithelial neoplasia (HGIN), advanced esophageal carcinoma (AEC) and normal control (NOR). All serum samples were pretreated with weak cation exchange (WCX) magnetic beads and tested by MALDI-TOF-MS, finally construction of boosting decision tree and blinded testing. Results There were 146 protein peaks were detected between the three parents groups compared with normal, the decision tree classification were constructed by 14 protein peaks, which contain 32721, 3403, 1772, 1071, 1414, 33312, 32023, 31043, 4662, 2245, 1307, 10013, 6868, 1234. This decision algorithm correctly classified 90.91%(30/33) of normal control, 100% (31/31) of LGIN, 89.66% (52/58) of HGIN, and 81.26% (26/32) of AEC.In the blinded testing, the decision algorithm correctly classified 95.652%(22/23) of normal control, 95.455%(21/22) of LGIN, 94.118%(32/34) of HGIN, and 95.238%(20/21) of AEC. The peaks of 32023, 1234 and 1307 were speculated zinc alpha2 glycoprotein, glycosyltransferase like protein LARGE2 fibrinogen alpha chain and fibrinogen alpha chain by ExPasy database retrieval, others were unknown. Conclusion The diagnostic pattern are established by differently protein peaks could accurately recognize the patients groups and normal controls application of MALDI-TOF MS with high sensitivity and specificity. We speculate that the occurrence of esophageal carcinoma began in the LGIN or much earlier, the patients that have cancer-related proteins in serum should be follow up.%目的:应用质谱技术构建

  10. Prevalence of high-grade squamous intraepithelial lesions and cervical cancer among patients with unsatisfactory colposcopic examination, without visible lesion

    OpenAIRE

    Fernanda Rangel da Veiga; Fábio Bastos Russomano; Maria José de Camargo; Aparecida Cristina Sampaio Monteiro; Aparecida Tristão; Gabriela Villar e Silva

    2009-01-01

    CONTEXT AND OBJECTIVE: Cervical cancer is a serious public health problem in Brazil. For patients with unsatisfactory colposcopic examinations without visible lesions, but with cervical cytological tests suggesting high-grade squamous intraepithelial lesion (HSIL), the national recommendation is to repeat cervical cytological tests after three months. Our aim was to assess the prevalence of HSIL and cancer among patients with initial cervical cytological tests suggestive of HSIL but with unsa...

  11. Preliminary results from multicenter clinical trials for detection of cervical squamous intraepithelial lesions using a novel full-field evoked-tissue-flourescence-based imaging instrument

    Science.gov (United States)

    Dattamajumdar, Anupam K.; Wells, D.; Parnell, Julie R.; Ganguly, Dipankur; Wright, Thomas C., Jr.

    2001-10-01

    We report preliminary results from a multi-center trial of evaluating the performance of a novel, full-field multi-spectral tissue fluorescence imaging system (CerviscanTM) designed to detect cervical squamous intraepithelial lesions. Spectral data from multiple regions of 48 patients enrolled from clinical performance sites in the US and Canada are included in this preliminary analysis. This includes 30 women used for the training set and 18 used for the testing set. In the testing set, 37/43 SIL and 70/80 NonSIL regions were correctly identified for a sensitivity of 86% and specificity of 87.5%. CerviscanTM locates cervical precancerous lesions with high sensitivity and specificity and has the potential to permit 'see and treat' patient management.

  12. Nd:YAG激光对胃癌癌前病变的阻断治疗%Nd:YAG laser treatment of precancerous lesion of gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    陈祖林; 罗云生; 唐建民; 刘作金

    2000-01-01

    We treat 23 cases with precancerous lesions of gastric carcinomas by endoscopic Nd:YAG laser plus oral colloidal bismuth subcitrate in therapy group whose effective rate is 86.4%(19/22) and control group's effective rate is 40.4%(6/15) in which only oral C.B.S.Was given.There is significant difference stataitically in two groups.It's suggested that Nd:YAG laser is a important method to prevent precancerous lesion of gastric carcinoma%采用内镜下Nd:YAG激光加口服丽株得乐做为治疗组治疗22例胃粘膜癌前病变,有效率为86.4%(19/22),而对照组15例单纯口服丽株得乐的疗效为40.0%(6/15),两组疗效有显著差异。说明Nd:YAG激光是阻断胃癌癌前病变的有效手段。

  13. Effect of Jinguo Weikang Capsule(金果胃康胶囊) on Proto-oncogene Expression of Gastric Mucosa in Rats with Gastric Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective:To study the effect of Jinguo Weikang Capsule (金果胃康胶囊,JWC) on the gene expression of H-ras,epidermal growth factor receptor (EGFR),P53 and C-myc of the gastric mucosa in rats with gastric precancerous lesions,and to investigate the action mechanism of JWC on gastric precancerous lesions.Methods:A rat model with paratypical proliferation of the gastric epithelium mucosa was established by using 60Co irradiation.Rats were divided into the normal group,model group,high-,medium-,low-dose JWC treatment groups,and the vitacoenzyme control group,and were treated for 30 days.The expression of H-ras,EGFR,P53 and C-myc genes of the gastric mucosa was detected by using immunohistochemical methods.Results:The expression and over-expression rates of H-ras,EGFR,P53 and C-myc gene in the high- and medium-dose JWC treatment groups were significantly lower (P<0.05)as compared with those of the model group.Conclusion:JWC can inhibit the expression of the H-ras,EGFR,P53 and C-myc genes expression of the gastric mucosa in rats,which may be one of mechanisms involved in suppressing or reversing gastric carcinogenesis.

  14. Microbiological characterization and effect of resin composites in cervical lesions

    Science.gov (United States)

    Carlo, Bonfanti; Piccinelli, Giorgio; Faus-Matoses, Vicente; Cerutti, Antonio

    2017-01-01

    Background Non carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect, in vivo and in vitro, against S. mutans. Material and Methods Eight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions. For each abfraction three samples of plaque were taken from the cervical perimeter: before the restoration, one week and three months after restoration. The samples were evaluated both by traditional microbiological methods and by Polymerase Chain Reaction (PCR). In vitro, disk-shaped specimens of the two composites were prepared to estimate the effects against pre-cultured S. mutans, after incubation at 37°C for 24h and assessed by a turbidimetric technique. Results In vivo no differences were found in plaque growth, for all samples, before and after restoration with both composites; in vitro, instead, a significant reduction of S. mutans growth was found between specimens of two composites (Mann-Whitney U-test p>0,06). Conclusions In this study a relevant consideration was elicited: composite materials, in vivo, do not modify plaque composition of non carious cervical lesions to a potential cariogenic plaque. Key words:Abfraction, restoration, S. mutans, composite, class V. PMID:28149461

  15. Co-expression of metalloproteinases 11 and 12 in cervical scrapes cells from cervical precursor lesions.

    Science.gov (United States)

    Valdivia, Alejandra; Peralta, Raúl; Matute-González, Manuel; García Cebada, Juan Manuel; Casasola, Ivonne; Jiménez-Medrano, Cristina; Aguado-Pérez, Rogelio; Villegas, Vanessa; González-Bonilla, Cesar; Manuel-Apolinar, Leticia; Ibáñez, Miguel; Salcedo, Mauricio

    2011-01-01

    The metalloproteinases (MMP) 11 and 12 have been shown to be expressed in cervical cancer (CC). In order to extend our previous results, these MMPs were evaluated in cervical precursor lesions. One hundred seventeen cervical scrapes: thirty-six normal, thirty-six low grade squamous lesions (LSIL), thirty-six high grade (HSIL), nine CC; and, also ninety-nine paraffin-embedded cervical lesions: fifteen normal cervices, thirty eight LSIL, sixteen HSIL, and five CC were collected. The samples were analyzed for relative expression by real time RT-PCR or immunohistochemistry assay. We were able to identify a relative increased expression of MMP11 in 75% and 78% from LSIL and HSIL samples, respectively. While MMP12 expression was 64% and 75% in LSIL and HSIL, respectively. Positive samples for MMP11 expression were also positive for MMP12 expression and also increased according to illness progression. In the tissues, MMP11 or MMP12 expression was observed in the cytoplasm of the neoplastic cells, while in the normal epithelium was absent. The reaction was always stronger for MMP12 than MMP11. MMP11 expression was present in 77% and 66% of LSIL and HSIL, while MMP12 expression was 73% and 68%. There was a relationship between MMP11 or MMP12 expression and HPV infection. Our data are showing a relationship between diagnostic of precursor lesions and the MMP11 and 12 expressions, suggesting that their expression could be an early event in the neoplastic lesions of the cervix and could have clinical significance.

  16. Vaginal Infections of Albanian women Infected with HPV and their impact in intraepithelial cervical lesions evidenced by Pap test

    Science.gov (United States)

    Kone, Elsa S; Balili, Avenir D; Paparisto, Piro D; Ceka, Xheladin R; Petrela, Elizana D

    2017-01-01

    Background: Cervical cytology is the best single method for large screening of the population in identifying precancerous lesions of the uterine cervix. Aim: To estimate the frequency of human papillomavirus (HPV) positivity in a group of Albanian women, the prevalence of vaginal coinfections, and the relationship of coinfections with HPV, as well as their role in metaplasia or cervical intraepithelial lesions (CIN). Materials and Methods: In this retrospective study, 2075 vaginal smears were examined. The Papanicolaou stain was used for all slides. The New Bethesda System 2001 was used for the interpretations of the smears. Data analysis was completed using the Statistical Package for the Social Sciences version 19.0. Results: Prevalence of HPV positivity was 43.9% with an average age of 35.48 ± 9.27 years. Candida coinfection resulted in 57.8% of HPV positive women with a significant relationship between them. Gardnerella coinfection resulted in 36 (23%), mixed flora in 34 (8%), and Trichomonas vaginalis in 50% of HPV positive woman. Among the women with positive HPV, 19% had CIN, 8% had metaplasia, and 1% had metaplasia and CIN; 9% of the women with HPV had CIN1 and one of the coinfections. Conclusions: There is a strong relationship between CIN1 and HPV positivity as well as between CIN1 and coinfections. HPV infection is a major factor contributing to metaplasia, and bacterial coinfections in HPV positive women have a statistically significant impact in the development of metaplasia. PMID:28182076

  17. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, Mandy; Scholtka, Bettina, E-mail: scholtka@uni-potsdam.de [Chair of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, Arthur- Scheunert-Allee 114-116, 14558 Nuthetal (Germany); Gottschalk, Uwe [Maria Heimsuchung Caritas-Klinik Pankow, Breite Straße 46/47, 13187 Berlin (Germany); Faiss, Siegbert [III. Medizinische Abteilung - Gastroenterologie und Hepatologie, Asklepios Klinik Barmbek, Rubenkamp 220, 22291 Hamburg (Germany); Schatz, Daniela; Berghof-Jäger, Kornelia [BIOTECON Diagnostics GmbH, Hermannswerder Haus 17, 14473 Potsdam (Germany); Steinberg, Pablo, E-mail: scholtka@uni-potsdam.de [Chair of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, Arthur- Scheunert-Allee 114-116, 14558 Nuthetal (Germany); Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover (Germany)

    2010-12-29

    In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567) by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study) and neoplastic lesions (in a previous study) of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer.

  18. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

    Directory of Open Access Journals (Sweden)

    Daniela Schatz

    2010-12-01

    Full Text Available In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567 by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study and neoplastic lesions (in a previous study of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer.

  19. Ultrastructure and molecular biological changes of chronic gastritis, gastric cancer and gastric precancerous lesions: a comparative study

    Institute of Scientific and Technical Information of China (English)

    Goang-Yao Yin; Wu-Ning Zhang; Xiao-Jing Shen; Yi Chen; Xue-Fen He

    2003-01-01

    AIM: To carry out a comparative study on ultrastructure and molecular biological changes of chronic gastritis (CG),gastric cancer (GC) aand gastric precancerous lesions.METHODS: By the use of histochemical staining, SEM with EDAX, TEM with EDAX, image analysis technique, RIA and chemiluminescence method, gastric mucosa of 168 patients were synchronously analyzed in morphology, trace elements, DNA, cAMP, SOD, 3H-TdR LCT and serum LPO were also done.RESULTS: The incidence of epithelial nucleoplasmic ratio >1, lobulated nuclei, inter-chromatin aggregation of granules, nucleolar hypertrophy, and the content of DNA,Zn, Cu in nuclei and serum LPO of each group were showed as belows: normal control group (0.0, 0.0, 6.7, 0.0, 12.6±2.7,7.6±0.4, 58.4±0.3, 2.6±0.6), CSG group (5.7, 2.9, 7.4, 2.9,15.2±3.1, 8.1±0.5, 58.9±0.5, 4.2±0.7), CAG group (31.3,29.7, 45.3, 42.2, 16.5±3.1, 8.6±0.4, 59.3±0.5, 4.5±0.6), CA group (100.0, 100.0, 72.2, 50.0, 30.7±8.2, 8.8±0.3, 59.5±0.4,6.8±1.6), ATP++group (61.5, 38.5, 23.1, 38.5, 23.5±8.9,8.3±0.4, 59.1±0.4, 5.1±1.2), IM+++ ATP++group (77.8, 55.5,33.3, 44.4, 25.1±7.2, 8.4±0.5, 59.5±0.4, 6.5±1.1),IM++++ATP++ group (100.0, 100.0, 75.0, 62.5, 28.5±9.1,8.9±0.5, 59.7±0.4, 7.6±0.7), IMⅡb group (100.0, 62.5, 75.0,50.0, 27.3±10.3, 8.6±0.3, 59.5±0.4, 6.1±0.9); whereas the content of Zn, Cu in mitochondria and cAMP, SOD in gastric mucosa, and 3H-TdR LCT of each group were showen as belows: normal control group (9.2±0.5, 58.3±0.3, 15.9±1.5,170.5±6.1, 1079.7±227.4), CSG group (8.6±0.5, 57.8±0.3,14.6±1.8, 163.3±5.6, 867.3±240.5), CAG group (8.3±0.4,57.5±0.3, 13.4±1.8, 161.2±4.3, 800.9±221.8), CA group (8.9±0.4, 57.1±0.3, 10.2±3.9, 152.2±3.8, 325.7±186.8),ATP++ group (9.1±0.4, 57.0±0.3, 12.4±1.8, 161.5±3.8,642.9±174.3), IM+++ ATP++ group (8.6±0.4, 56.9±0.3,12.0±2.3, 152.2±2.5, 326.3±160.3), IM++++ATP++ group (8.5±0.3, 56.8±0.2, 10.4±0.9, 147.4±2.6, 316.1±170.7),IMⅡb group (8.6±0.3, 56

  20. REPRODUCTIVE ASPECTS ASSOCIATED WITH PRECURSOR LESIONS FOR CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Rogério Ferrari

    2013-05-01

    Full Text Available To investigate the relationship between reproductive variables and theprecursor lesions for cervical cancer in women attended the clinic of the lower genitaltract pathology and colposcopy (PTGIC, packed in the complex regional healthCaceres city, southwest of Mato Grosso, in the year 2009.Methods:We conducted across sectional study with data collection from medical records of 142 women withabnormal cytology, colposcopy and positive underwent directed biopsy, taking intoaccount the reproductive variables.Results:indicate that the minimum age at menarchewas 9 years and maximum age was 17 years, mean 12.6 years; on the average paritywas 3.4 children, the use of hormonal contraceptive method corresponds to 34 5% and38.1% female sterilization research, 46.5% are or have use of contraception for a periodgreater than five years, with a mean of 4.7 years and 67.2% of women surveyed do notuse condoms. The variables were not significantly associated to cervical cancer.Conclusion:the characteristics of the women studied may serve as a basis for workdirected to this population in order to seek to minimize this problem.Although the dataobtained were satisfactory, it was possible to trace the profile of the reproductiveaspects of women in the Clinic ofPTGIC, there is a lack of association betweenreproductive aspects and findings of the cervical biopsy, it may be associated with somelimiting factors of the study that need to be balanced

  1. Detecting uterine glandular lesions: Role of cervical cytology

    Directory of Open Access Journals (Sweden)

    Baneet Bansal

    2016-01-01

    Full Text Available Background: The sensitivity of cervical cytology for detection of glandular lesions is reported to be low. We conducted this study to assess the diagnostic accuracy of cervical Papanicolaou (Pap smears for uterine glandular lesions and to compare the diagnostic utility of conventional and liquid-based cytology (LBC smears for glandular lesions. Materials and Methods: Archived histopathology records of all cases reported as endocervical and endometrial adenocarcinoma in the study period were identified and the available corresponding Pap smears (in preceding 1 year were retrieved. In addition, the Pap smears reported as glandular cell abnormalities (GCA during the same study period were retrieved. The overall prevalence of GCA, sensitivity, and specificity of Pap smears for the detection of GCA was calculated. The diagnostic accuracy of conventional and LBC smears for the diagnosis of GCA was also compared. Results: The prevalence of GCA in our study was 0.32%. The overall specificity of Pap smears for the diagnosis of GCA was 60.8%, this was not significantly different between conventional and LBC smears (P = 0.4. The overall sensitivity of Pap smears for the detection of GCA was 41.8%; LBC smears had significantly better sensitivity as compared to conventional smears for the detection of endometrial as compared to endocervical adenocarcinoma (P < 0.05. Conclusions: The prevalence of GCA in Pap smears is low. The specificity of Pap smears, for diagnosis of GCA, was found to be moderate. However, the overall sensitivity of Pap smears for the detection of GCA was low, though better for LBC as compared to conventional smears.

  2. Cervical Lesions in Women Younger than 25 Years

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a diciembre de 2007 y primer trimestre del 2008. Se analizaron: edad, edad de primera relación sexual, número de parejas sexuales, tipo de lesión, grado de la lesión y motivo de consulta. Resultados: predominaron las mujeres de más de 21 años y las que tuvieron 2 y 3 parejas sexuales; el principal motivo de consulta fue la cervicitis (84, 7 %; en el 22,6 % de las mujeres se detectó neoplasia intraepitelial grado I, en el 41, 9 % de grado II y en el 33, 1 % de grado III. En el 90 % de las pacientes más jóvenes la lesión fue  maligna. Conclusiones: la presencia de lesiones de cuello uterino en mujeres menores de 25 años no es baja, como tampoco la frecuencia de lesiones malignas.

  3. HPV-genotypes in high-grade intraepithelial cervical lesions in Danish women

    DEFF Research Database (Denmark)

    Kirschner, Benny; Schledermann, Doris; Holl, Katsiaryna;

    2013-01-01

    A study was undertaken to assess the distribution of high-risk HPV-genotypes in high-grade cervical intraepithelial neoplastic lesions in Danish women.......A study was undertaken to assess the distribution of high-risk HPV-genotypes in high-grade cervical intraepithelial neoplastic lesions in Danish women....

  4. Value of Narrow Band Imaging (NBI) Endoscopy in the Early Diagnosis of Laryngeal Cancer and Precancerous Lesions

    Science.gov (United States)

    2016-09-07

    Chronic Inflammation of Hypopharynx; Hoarseness; Macroscopic Laryngeal Lesions; Histological Confirmation of Dysplasia; Carcinoma in Situ; Invasive Carcinoma of Larynx; Invasive Carcinoma of Hypo Pharynx; Recurrent Respiratory Papillomatosis

  5. CO2 laser application in gynecology: experience in microsurgery of cervical lesions

    Science.gov (United States)

    Knapp, Piotr A.

    1995-03-01

    A CO2 laser device was used for treating cervical lesions in 1574 patients. Of the total, 163 were diagnosed as CIN. Patients were selected for the study as a result of mass screening during the period from 1988 to 1992 of Bialystok Province, Poland. Treatment of cervical lesions with laser proved to be effective. In the author's opinion it is an essential step in preventing cervical malignancy.

  6. TCT联合HPV检测在宫颈病变筛查中的意义%Cervical Liquid-Based Cytology Combined Human Papillomavirus Testing in Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    戴一菲; 田荣华

    2012-01-01

    Objective To study the significance of combination of cervical liquid-based cytology and human papillomavirus detection in cervical lesion screening. Methods From March 2010 to December 2011,3,028 patients were detected with nipple shaped tumor virus ( HPV) and cervical liquid base cytology (TCT) ,TCT was used by 2001 International Cancer Association TBS diagnosis system report way,to the patients with positive results of TCT check for ASCIIS and the above lesions and (or) HPV detection, cervical organization biopsy under electronic vaginal mirror were performed. Results The sensitivity of TCT check was 56.72% ,with the specificity of 28. 19% ;the sensitivity of HPV check was 86.03% ,with specific for 46.38% ;among 408 cases of CIN and cervical cancer pathology ,28 cases were single TCT positive (6. 86% ) ;with TCT and HPV positive 203 ca-ses(49. 75% ) ,there was significant difference in diagnosis of cervical squamous intraepithelial neoplasm ( CIN) and cervical cancer(x2 = 184.926,P=0.000) between single TCT check and combination of TCT and HPV examination. Conclusion TCT combined with HPV check can improve the detection rate of cervical cancer and precancerous lesions, improve the sensitivity and specificity of screening,effectively reduce the TCT in detection of false-negative rates,and avoid excessive colposcopy and biopsy in low-risk populations and over-treatment.%目的 探讨宫颈液基细胞学检查与人乳头状瘤病毒检测联合应用在宫颈病变筛查中的意义.方法 选取2010年3月-2011年12月孝感市中心医院病理科同时进行了宫颈液基细胞学(TCT)检查及人乳头状瘤病毒(HPV)检测的患者3028人,TCT采用2001年国际癌症协会TBS诊断系统报告方式,对TCT检查结果为ASCUS及以上病变和(或)HPV检测结果为阳性的患者在电子阴道镜下取宫颈组织活检.结果 TCT检查敏感度为56.72%,特异度为28.19%;HPV检查敏感度为86.03%,特异度为46.38%;病

  7. Expression of Inducible Nitric Oxide Synthase, p53 and Bcl-2 in Gastric Precancerous and Cancerous Lesions: Correlation with Clinical Features

    Institute of Scientific and Technical Information of China (English)

    Tao Cui; Zu'an Zhu; Ying Liu; Qingyan Kong; Sujuan Fei

    2006-01-01

    OBJECTIVE To explore the expression of inducible nitric oxide synthase(iNOS), p53 and bcl-2 in gastric precancerous and cancerous lesions and to examine the expression of these proteins in relation to clinical features.METHODS The expressions of iNOS, p53 and bcl-2 proteins in gastric precancerous and cancerous lesions and their correlations with the clinical features were determined using immunohistochemical assays (Power VisionTM two-step method) on 84 gastric carcinomas and 54 gastric atypical hyperplastic tissues. Apoptotic cells were evaluated by terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick-end labeling (TUNEL).RESULTS Expression of iNOS, p53 and bcl-2 was significantly higher in gastric carcinoma (GC) tissues than in gastric atypical hyperplastic tissues. Among the 84 carcinomas, the expression of p53 was observed in 50 (59.52%), bcl-2 in 43 (51.19%), and iNOS in 65 (77.58%). Overexpression of iNOS and bcl-2 in gastrlc carcinoma was related to tumor size and iNOS was related to the presence of lymph node metastasis (P<0.05). The expression of proteins did not correlate with age, sex, stage of disease, or differentiation. Expression of iNOS in gastric carcinoma tissues was positively correlated with bcl-2 expression (χ2=8.926, P=0.003),and also with p53 expression (χ2= 5.2430, P= 0.022). The mean apoptotic indexes (Al) were 1.29%±0.50 in low-grade atypical hyperplasia (LG),0.96%±0.36 in high-grade atypical hyperplasia (HG) and 0.70%±0.43 in GC, with the difference being significant between LG, HG and GC (P<0.05). There was a significant positive correlation between iNOS expression and the Al in GC (t=3.0815, P=0.0028).CONCLUSION iNOS was expressed in the majority of gastric carcinoma tissues and correlated with cellular apoptosis associated with p53 and bcl-2 expression. iNOS overexpression is closely associated with p53 and bcl-2 accumulation status. iNOS may play a synergistic role in the pathogenesis of GC.

  8. Treatment Progrest of Gastric Precancerous Lesions in Traditional Chinese Medicine%胃癌前病变的中医药治疗进展

    Institute of Scientific and Technical Information of China (English)

    谢晶日; 孙涛; 张杨

    2012-01-01

    近年来,中医药在治疗胃癌前痛变方面取得了许多令人满意的效果.复习近几年的文献,详细论述了本病发生的病因病机,以及其基本治疗原则.总结大多数学者的观点将本病的临床分型分为“脾胃虚弱型,肝郁气滞型,胃阴不足型,其他型”,并列举出具有代表性的医家的治疗方法及治疗结果.同时也分析了中医药在治疗本病方面的不足及未来努力的方向.%In recent years, Chinese medicine in the treatment of precancerous lesion of gastric cancer has made many satisfactory results. The article reviews the recent literatures, discusses in detail the disease etiology and pathogenesis, as well as the basic principle of treatment. Most scholars view the disease clinical classification into the weakness of spleen and stomach, liver qi depression, stomach yin deficiency type and other type, and list out the representative medical treatment methods and treatment results. It also analyzes the disadvantages and future direction.

  9. Chromosome 11 aneusomy in esophageal cancers and precancerous lesions-an early event in neoplastic transformation: An interphase fluorescence in situ hybridization study from south India

    Institute of Scientific and Technical Information of China (English)

    Vasavi Mohan; Shivani Ponnala; Hemakumar M Reddy; Radha Sistla; Rachel A Jesudasan; Yog Raj Ahuja; Qurratulain Hasan

    2007-01-01

    AIM: To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies.METHODS: We performed fluorescence in situ hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control)obtained from patients referred to the clinic for endoscopic evaluation.RESULTS: Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers.CONCLUSION: Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers.

  10. Lobular neoplasia found on breast biopsy: marker of increased risk of malignancy or direct pre-cancerous lesion?

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    Jan Kornafel

    2011-10-01

    Full Text Available The aim of this study was to compare the imaging symptoms and microscopic findings in females with lobular neoplasia (LN found on biopsy. 1,478 women who underwent primary open biopsy or surgical excision after percutaneous biopsy were reviewed. In 24 of them (1.6%, LN was found. In four patients, excisional biopsy with hook-wire localization was done primarily due to the radial scar. In 20 females, surgical excision of BIRADS 4 lesion was performed because of the presence of LN in specimens from the vacuum-assisted or core-needle percutaneous biopsy. Postoperative pathologic findings were compared to the radiological symptoms. In 13 women, LN did not produce any radiological symptoms and was an additional histologic finding existing near the other lesion: fibroadenoma and radial scar. In none of these lesions was an invasive cancer noticed. In one single patient, ductal carcinoma in situ was observed in the other segment of the breast. Invasive ductal cancer developed in the contralateral breast in one patient. In 11 patients, LN was diagnosed due to radiological symptoms produced by itself. In this group, the invasive lobular cancer was found in seven lesions (64%. Our finding suggests that LN producing suspicious radiological symptoms can be a different biologic type of this lesion when compared asymptomatic LN diagnosed which is usually found on biopsy as additional microscopic pathology. Symptomatic LN is probably associated with a higher potential of malignant transformation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 417–424

  11. Human telomerase RNA component (hTERC gene amplification detected by FISH in precancerous lesions and carcinoma of the larynx

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    Liu Yu

    2012-03-01

    Full Text Available Abstract Background Gain of 3q26 is frequently observed in squamous cell carcinomas of mucosal origin, including those originating in the head and neck region. The human telomerase RNA component (hTERC gene, which is located on chromosome 3q26, encodes for an RNA subunit of telomerase that maintains the length of telomeres through cellular divisions, and is activated in malignant diseases. The present study was designed to detect hTERC amplification in laryngeal lesions and evaluate whether this might serve as a supportive biomarker in histopathological analysis for in the diagnosis of laryngeal lesions. Methods Fluorescent in situ hybridization (FISH was applied on formalin-fixed paraffin-embedded blocks of 93 laryngeal specimens, including 14 normal epithelium (NE, 15 mild dysplasia (Md, 18 moderate dysplasia (MD, 16 severe dysplasia (SD, 9 carcinoma in situ (CIS, and 21 invasive carcinoma (IC. Results By histopathologic examination, hTERC amplification rates in NE, Md, MD, SD, CIS and IC cases were 0% (0/14, 13.33% (2/15, 72.22% (13/18, 81.25% (13/16, 100% (9/9 and 100% (21/21, respectively. Amplification of hTERC was significantly associated with histopathologic diagnosis (P Conclusions The hTERC amplification is important in the development of laryngeal squamous cell carcinoma (LSCC. FISH detection of hTERC amplification may provide an effective approach in conjunction with histopathologic evaluation for differential diagnosis of laryngeal lesions. Virtual Slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2226606266791985

  12. Immunohistochemical expression of p16(INK4a) is predictive of HR-HPV infection in cervical low-grade lesions.

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    Benevolo, Maria; Mottolese, Marcella; Marandino, Ferdinando; Vocaturo, Giuseppe; Sindico, Roberto; Piperno, Giulia; Mariani, Luciano; Sperduti, Isabella; Canalini, Paola; Donnorso, Raffaele P; Vocaturo, Amina

    2006-03-01

    The p16(INK4a) is a cyclin-dependent kinase inhibitor that decelerates the cell cycle by inactivating the cyclin-dependent kinases involved in the phosphorylation of the retinoblastoma protein (RB). Expression of E6 and E7 oncogenes of high-risk (HR) human papillomavirus (HPV), affecting the RB-p16 pathway, leads to p16 upregulation. Although it is widely reported that p16 is overexpressed in a high percentage of preneoplastic lesions and in almost all carcinomas of the uterine cervix, protein upregulation and its correlation with HPV infection in low-grade lesions is still being debated. In this study, we investigated in parallel, p16 expression and HPV infection in 100 cervical biopsies (17 normal tissues, 54 CIN1, 10 CIN2, 11 CIN3, eight invasive squamous cancers). Results obtained demonstrated that none of the 17 normal cervical tissues, evaluated by immunohistochemistry, presented p16 positivity whereas, starting from CIN1 (31%) to CIN2 (90%), CIN3 (100%) and carcinomas (100%), a constant and significant increase of protein overexpression (P<0.0001) was observed. In addition, p16 overexpression consistently showed elevated sensitivity (84%) and specificity (98%) in detecting HR-HPV infection with a high positive predictive value (97%) and negative predictive value (86%). Of interest, 93% of the p16-positive CIN1 were also HR-HPV infected. Our findings confirmed that p16 overexpression is associated to high-grade precancerous lesions and cervical carcinomas, and further demonstrated that immunohistochemical evaluation of p16 may be a useful biomarker in identifying HR-HPV-infected low-grade lesions.

  13. Mutant KRas-Induced Mitochondrial Oxidative Stress in Acinar Cells Upregulates EGFR Signaling to Drive Formation of Pancreatic Precancerous Lesions

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    Geou-Yarh Liou

    2016-03-01

    Full Text Available The development of pancreatic cancer requires the acquisition of oncogenic KRas mutations and upregulation of growth factor signaling, but the relationship between these is not well established. Here, we show that mutant KRas alters mitochondrial metabolism in pancreatic acinar cells, resulting in increased generation of mitochondrial reactive oxygen species (mROS. Mitochondrial ROS then drives the dedifferentiation of acinar cells to a duct-like progenitor phenotype and progression to PanIN. This is mediated via the ROS-receptive kinase protein kinase D1 and the transcription factors NF-κB1 and NF-κB2, which upregulate expression of the epidermal growth factor, its ligands, and their sheddase ADAM17. In vivo, interception of KRas-mediated generation of mROS reduced the formation of pre-neoplastic lesions. Hence, our data provide insight into how oncogenic KRas interacts with growth factor signaling to induce the formation of pancreatic cancer.

  14. Prevalence of high-grade squamous intraepithelial lesions and cervical cancer among patients with unsatisfactory colposcopic examination, without visible lesion

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    Fernanda Rangel da Veiga

    Full Text Available CONTEXT AND OBJECTIVE: Cervical cancer is a serious public health problem in Brazil. For patients with unsatisfactory colposcopic examinations without visible lesions, but with cervical cytological tests suggesting high-grade squamous intraepithelial lesion (HSIL, the national recommendation is to repeat cervical cytological tests after three months. Our aim was to assess the prevalence of HSIL and cancer among patients with initial cervical cytological tests suggestive of HSIL but with unsatisfactory colposcopic examinations without visible lesions, in order to contribute towards the discussion regarding a more effective clinical approach that might diminish the likelihood of patient abandonment of follow-up before appropriate diagnosis and treatment. DESIGN AND SETTING: Cross-sectional study in Colposcopy Clinic of IFF/Fiocruz. METHOD: Patients admitted between December 1989 and April 2007 with cytological diagnoses of HSIL but with unsatisfactory colposcopic examinations without visible lesions underwent cervical cone biopsy. RESULTS: Sixty-five such patients were included, comprising 33.8% with HSIL and 4.6% with cancer, confirmed histologically. The other patients presented low-grade squamous intraepithelial lesion (26.1%, glandular dysplasia (1.5% and absence of disease (33.8%. CONCLUSION: The observed prevalence of cancer and HSIL does not seem to be enough to justify immediate referral for cone biopsies to investigate the cervical canal in these cases. The findings suggest that the recommendation of repeated cytological tests following an initial one with HSIL, among patients with unsatisfactory colposcopic examinations without visible lesions, is appropriate in our setting. Efforts are needed to ensure adherence to follow-up protocols in order to reduce the chances of losses.

  15. Detection of the tumor markers in patients with gastric precancerous lesions%胃癌癌前病变相关标志物的检测

    Institute of Scientific and Technical Information of China (English)

    刘中娟; 程歆琦; 郭子建; 赵丽娜; 邱玲

    2013-01-01

    It is confirmed that chronic atrophic gastritis (CAG) caused by Helicobacter pylori is the main cause of gastric precancerous lesions.CAG is also the key determinant in gastric cancer risk assessment,which affects pepsinogen and gastrin-17 secretion.Most of the gastric cancer patients have poor prognosis,and non-invasive tools for gastric cancer screening and diagnosis are lacking.Therefore,the early detection of gastric cancer in order to reduce the disease mortality is necessary.Pepsinogen and gastrin-17 are biomarkers of gastric mucosa and gastric antra.The serological testing for the stomach-specific biomarkers offers the possibility to know preneoplastic gastric mucosal conditions.%研究证实,幽门螺旋杆菌感染引起的慢性萎缩性胃炎是诱发胃癌的主要癌前病变,是胃癌风险评估的关键并影响胃蛋白酶原(PG)和胃泌素-17(G-17)分泌.大多数胃癌患者预后差,且缺乏非侵袭性的筛查和诊断手段,因此早期发现胃癌以降低胃癌病死率非常必要.PG和G-17分别为胃体黏膜和胃窦的生物标志物,这些特异性生物标志物的检测为了解肿瘤发生前胃黏膜的状况提供了可能.

  16. The clinical significance of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers%液基细胞学用于外来工妇女宫颈癌前病变筛查的临床意义

    Institute of Scientific and Technical Information of China (English)

    卢晓艳; 谢家滨; 杨步琴

    2011-01-01

    目的:探讨液基细胞学检查用于外来工妇女宫颈癌前病变筛查中的诊断价值及宫颈癌筛查现状.方法:应用液基细胞学(TCT)对到我院门诊就诊的外来工妇女(外来组)2 500名和进行常规宫颈癌前病变筛查的常住妇女1 600名(本地组)进行对比,并对其中细胞学筛查异常者进行阴道镜及镜下宫颈活检病理检查.结果:外来工妇女中,检测前有阴道排液或性生活出血等症状者878例,占35.12%,本地组有症状者87例,占5.43%.外来组共检测出细胞阳性368例,占14.72%,本地组异常例数为110例,占6.87%,两组比较,差异有统计学意义(P<0.05).对细胞学检查异常者进行阴道镜及镜下宫颈活检病理检查,其中CINⅠ级以上360例,符合率达75.3%.结论:门诊就诊的外来妇女与本地妇女相比较,宫颈癌前病变发病率较高,有症状者来院就诊比率明显升高,外来组妇女对宫颈癌前病变筛查认识普及率低下,因此常规对外来工就诊妇女进行液基细胞学检查,并结合阴道镜宫颈活检,是筛查和诊断宫颈癌前病变的可靠手段.%Objective: To discuss the diagnostic value of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers and to survey the status of screening tests for precancerous lesions of the uterine cervix. Methods: 2 500 female migrant workers who went to our hospital for treatment in the method of liquid based cytology (TCT) compared with 1 600 towns women who went to our hospital for routine examination in screening tests for precancerous lesions of the uterine cervix, then examined the women whose index were abnormal by means of colposcopy and cervical biopsy. Results: 878 female migrant workers had the symptoms of draining the liquid from vagina before examination and bleeding during intercourse, accounting for 35.12%; and 87 towns women had the symptoms, accounting for 5.43%. There were 368 cases

  17. A Correlative Analysis of Cervical Lesions in Patients with Vulva Condyloma Acuminatum

    Institute of Scientific and Technical Information of China (English)

    Qing Li; Weihong Li; Huanzi Li; Zhihua Liu

    2006-01-01

    OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions.METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, highrisk HPV-DNA test and cervical bioscopy.RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV (+) infections were detected in 68.7% (287/418) of the cases. Among those patients, 20.6% (59/287) had concurrent subclinical cervical intraepithelial neoplastic (CIN) lesions. Among the high-risk HPV(-) 31.3 % (131), patients 7.6% (10/131) had concurrent subclinical CIN lesions. Pathological examination results: cervicitis,167 (40.0%); cervical HPV infection, 182 (43.5%); CIN-Ⅰ, 51(12.2%); CIN-Ⅱ, 16 (3.83%); CIN-Ⅲ, 2 (0.5%); cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia (VIN) Ⅱ~Ⅲ.CONCLUSION It was concluded that simultaneous cervical HPV infection of many types was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.

  18. Glass ionomer cements and their role in the restoration of non-carious cervical lesions

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    Luciana Fávaro Francisconi

    2009-10-01

    Full Text Available Glass ionomer based materials are clinically popular in several areas of restorative dentistry, but restoration of cervical lesions has proven particularly successful. Various etiologies, conformations, locations and structural characteristics make non-carious cervical lesions more challenging to adhesive restorative procedures and marginal seal in the long run. Due to their characteristics, glass ionomer cements (GICs have precise indication for these cases. Moreover, the use of a GIC base underneath composite resin, the so-called "sandwich" or mixed technique, allows associating the good characteristics of composite resins and GICs, and has been considered quite useful in the restoration of non-carious cervical defects. The aim of this paper is to critically review the literature and discuss peculiar features of GICs regarding their role in the restoration of non-carious cervical lesions.

  19. Expression of gastric cancer-associated MG7 antigen in gastric cancer, precancerous lesions and H. pylori-associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Dong-Li Guo; Ming Dong; Lan Wang; Li-Ping Sun; Yuan Yuan

    2002-01-01

    AIM: To investigate the relationship between the antigen MG7 antigen expression and gastric cancer as well as precancerous condition; to study the relationship between the MG7 antigen expression and H. pyloriinfection in benign gastric lesions in order to find out the effect of H. pylori infection on the process of gastric cancer development.METHODS: The level of MG7 antigen expression was determined by immunohistochemical method in 383 gastric biopsied materials. The intestinal metaplasia was determined by histochemistry method. The H. pyloriinfection was determined by HE stain, PCR and ELISA in 291 specimens, among which only 34 cases of H. pylori-associated gastric lesions were followed up.RESULTS: The positive rate of MG7 expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric cancer increased gradually in ascending order (P<0.01). The positive rate of MG7 antigen expression in type Ⅲ intestinal metaplasia of gastric mucosa was higher than that of type Ⅰand Ⅱ intestinal metaplasia, being highly significant (P<0.05).The positive rate of MG7 antigen expression in superficial gastritis, atrophic gastritis and gastric cancer increased gradually (11.9 %, 64.8 %, 91.2 %, P<0.01). There was no significant difference between H.pylori-negative and H. pyloripositive intestinal metaplasia, atrophic gastritis and dysplasia of gastric epithelium in the positive rate of MG7 antigen expression. There was no expression of MG7 antigen in H. pylori-negative superficial gastritis. The positive rate of MG7 expression in H. pylori-positive superficial gastritis was 20.5 %, and the difference between them was significant (P<0.05). During following up, one of the three H. pylori negative cases turned positive again, and its MG7 antigen expression turned to be stronger correspondingly. 3 of 31 H. pyloripositive cases were detected as early gastric cancer, among which one with "+++" MG7 antigen expression was diminished after H. pylori

  20. 胃癌前病变转归影响因素回顾性队列研究%Influencing factors of reverse of gastric precancerous lesions:a retrospective cohort study

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    周亮; 赵瑶; 朱伟; 沈月平

    2013-01-01

    目的 研究胃癌前病变的转归情况及其影响因素,为胃癌前病变或胃癌的预防提供基础资料.方法 采用回顾性队列研究方法对江苏省溧阳市2004年前经病理确诊为胃癌前病变的患者进行随访,通过胃镜检查、病理诊断及问卷调查获取病理结局及相关影响因素.结果 122例胃癌前病变患者有41例转归为非胃癌前病变,转归率为33.61%;Cox回归模型分析显示,在调整年龄、性别、职业、吸烟、饮茶、饮酒等因素后,与不采取治疗措施的对象比较,采取治疗措施对象发生转归的RR值为3.24(95% CI=1.22~ 8.57),常规服药对象发生转归的RR值为3.92(95%CI=1.45 ~10.64),西药治疗对象发生转归的RR值为2.84(95% CI=1.08 ~7.46),中药治疗对象发生转归的为RR值为5.13(95% CI=1.22 ~21.74).结论 采取治疗措施和常规服药是促使胃癌前病变患者发生良性转归的重要因素.%Objective To study the reverse status of gastric precancerous lesions and its influencing factors.Methods Using retrospective cohort study,the patients diagnosed as gastric precancerous lesions by pathology in 2004 in Liyang city,Jiangsu province were followed up for 5 years to get the pathological outcome by endoscopy and information on relevant factors were collected with a questionnaire survey.Results Totally 41 of 122 patients (33.61%) were reversed from precancerous lesions to non-precancerous lesions.Cox regression analysis showed that after adjustment for age,sex,occupation,smoking,drinking tea and alcohol drinking,compared to non-treatment group,the treatment group had an increased predisposition of reversing precancerous lesions (relative risk [RR] =3.24 ; 95 % confidence interval [95 % CI]:1.22-8.57).The RR of the reverse for the patients adopting regular medication was 3.92 (95 % CI:1.45-10.64),the RR of reverse for the patients taking traditional Chinese medication was 5.13 (95% CI:1.22-21.74),and the RR for

  1. Prevalence of HPV DNA in cervical lesions in patients from Ecuador and Japan.

    Science.gov (United States)

    Paez, C; Konno, R; Yaegashi, N; Matsunaga, G; Araujo, I; Corral, F; Sato, S; Yajima, A

    1996-11-01

    Cervical cancer is about 6 times more frequent in Ecuador than in Japan. We investigated the association between infection by the human papillomavirus (HPV) and the genesis of cervical cancer in specimens of lesions of the cervical epithelium obtained from patients in Ecuador and Japan. We also examined the results of HPV DNA detection and typing by the polymerase chain reaction (PCR) performed under the same technical conditions in areas with differing rates of cervical cancer. Purified tissue DNA from paraffin-embedded samples was amplified by PCR with universal and type-specific primers. HPV DNA was detected in 8 (20%) of 40 normal cervical epithelial samples from Ecuadorian patients, 19 (45%) of 42 low-grade squamous intraepithelial lesions (LSIL), 16 (50%) of 32 high-grade squamous intraepithelial lesions (HSIL), and 38 (81%) of 47 invasive squamous cell carcinomas (SCC) compared with 3 (10%) of 30 normal cervical specimens from Japanese patients, 107 (51%) of 210 HSILs, and 45 (71%) of 63 SCCs. The prevalence of HPV types 16 and 18 rose significantly with increasing histological grade (p poverty and underdevelopment may influence the prevalence of HPV infection and the sequence of events after HPV infection culminating in cervical cancer. These factors may help to explain the differing geographic distribution of this disease.

  2. Computed tomography in the foramen magnum and high cervical cord lesion

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    Isu, T.; Ito, T.; Iwasaki, Y.; Tsuru, M. (Hokkaido Univ., Sapporo (Japan). School of Medicine); Kitaoka, K.

    1981-10-01

    The value of computed tomography in the spine and spinal cord disease recently has been well documented. However, little attention is made to the role of computed tomography in foramen magnum and high cervical cord lesion. We report 16 cases of the foramen magnum and high cervical cord lesion. Method: Either EMI Head Scanner, CT 1010 (slice thickness 10 mm) or EMI Whole Body Scanner, CT 5005 (slice thickness 13 mm) was used. In 9 cases enhanced CT with intravenous injection of contrast medium was performed. Eleven cases received intrathecal injection of metrizamide. Materials: 1) control group without cervical pathology 70 cases, 2) spinal cord tumor 6 cases, 3) atlanto-axial dislocation 6 cases, 4) Chiari malformation (type 1) 3 cases, 5) spinal foreign body (acupuncture needle) 1 case. Results: 1. plain CT. 1) In control group without cervical pathology the cervical cord at C/sub 1/ level was seen as area surrounded by ring of subarachnoid space. 2) In 14 cases except for 1 case of atlanto-axial dislocation and 1 case of spinal foreign body identification of the cervical cord at C/sub 1/ level was impossible. 3) CT was of diagnostic value in detecting altanto-axial dislocation and spinal foreign body. 2. enhanded CT. Meningioma and neurinoma showed positive contrast enhancement. However, astrocytoma and herniated cerebellar tonsils were not detectable. 3. CT metrizamide myelography visualized the subarachnoid space and made it possible to localize the lesion in the spinal canal in relation to the spinal cord. Conclusion: Attention should be given to plain CT finding at C/sub 1/ level in diagnosis of foramen magnum and high cervical cord lesion. If identification of cervical cord at C/sub 1/ level is impossible, it is suspected that subarachnoid space is blocked by the lesion, and enhanced CT and CT metrizamide myelography must be performed.

  3. Correlation analysis of high-risk human papillomavirus viral load and cervical lesions

    Directory of Open Access Journals (Sweden)

    Xiao-xing MA

    2012-05-01

    Full Text Available Objective  To explore the association between high-risk human papillomavirus (HR-HPV viral load and pathological grades of cervical intraepithelial neoplasia (CIN and cervical cancer. Methods  A total of 1248 patients from General Hospital of PLA, who underwent colposcopy and surgery due to cervical lesions between Jan. 2006 and Aug. 2011 were enrolled in this study, and they were divided five groups: cervicitis, CIN Ⅰ, CIN Ⅱ-Ⅲ, stage Ⅰ cervical cancer and stage Ⅱ cervical cancer. HR-HPV viral load (RLU/CO was determined by the Hybrid Capture Ⅱ (HCⅡ system, and they were categorized into five groups: 0-0.99, 1.00-9.99, 10.00-99.99, 100.00-999.99, ≥1000.00. The mean value and standard deviation of different HR-HPV viral load in the patients with cervicitis or with CIN Ⅰ, CINⅡ-Ⅲ, stage Ⅰ cervical cancer or stage Ⅱ cervical cancer were compared, and the correlation of HR-HPV viral load and pathogenesis of cervical lesions was analyzed. Results  HPV viral loads were significantly higher in CINⅠ(842.1±983.9, CINⅡ-Ⅲ (690.1±795.0, stage Ⅰ cervical cancer (893.1±974.2 and stage Ⅱ cervical cancer (699.5±908.3 patients than in cervicitis patients (274.2±613.6, P < 0.05, and the HPV viral loads in CINⅠ(842.1±983.9 and stage Ⅰ cervical cancer patients were higher than those in CINⅡ-Ⅲ patients (P < 0.05. When HR-HPV viral load was ≥100RLU/CO, the risk of CIN and cervical cancer increased with the increase in viral load, but there was no correlation between the viral load and pathological grades of cervical lesions. In the patients with stage ⅠB-Ⅱ cervical squamous cell carcinoma, when the HR-HPV viral load was ≥100RLU/CO, the risk of lymph node metastasis increased (P < 0.05, and the number of patients with maximum diameter of the cervical tumor ≥4cm also increased (P < 0.05. However, the HR-HPV viral load was not correlated with patient age, pathological type of the lesion, depth of cancer

  4. Human papillomavirus prevalence, viral load and pre-cancerous lesions of the cervix in women initiating highly active antiretroviral therapy in South Africa: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Rybicki Ed

    2009-08-01

    Full Text Available Abstract Background Cervical cancer and infection with human immunodeficiency virus (HIV are both important public health problems in South Africa (SA. The aim of this study was to determine the prevalence of cervical squamous intraepithelial lesions (SILs, high-risk human papillomavirus (HR-HPV, HPV viral load and HPV genotypes in HIV positive women initiating anti-retroviral (ARV therapy. Methods A cross-sectional survey was conducted at an anti-retroviral (ARV treatment clinic in Cape Town, SA in 2007. Cervical specimens were taken for cytological analysis and HPV testing. The Digene Hybrid Capture 2 (HC2 test was used to detect HR-HPV. Relative light units (RLU were used as a measure of HPV viral load. HPV types were determined using the Roche Linear Array HPV Genotyping test. Crude associations with abnormal cytology were tested and multiple logistic regression was used to determine independent risk factors for abnormal cytology. Results The median age of the 109 participants was 31 years, the median CD4 count was 125/mm3, 66.3% had an abnormal Pap smear, the HR-HPV prevalence was 78.9% (Digene, the median HPV viral load was 181.1 RLU (HC2 positive samples only and 78.4% had multiple genotypes. Among women with abnormal smears the most prevalent HR-HPV types were HPV types 16, 58 and 51, all with a prevalence of 28.5%. On univariate analysis HR-HPV, multiple HPV types and HPV viral load were significantly associated with the presence of low and high-grade SILs (LSIL/HSIL. The multivariate logistic regression showed that HPV viral load was associated with an increased odds of LSIL/HSIL, odds ratio of 10.7 (95% CI 2.0 – 57.7 for those that were HC2 positive and had a viral load of ≤ 181.1 RLU (the median HPV viral load, and 33.8 (95% CI 6.4 – 178.9 for those that were HC2 positive with a HPV viral load > 181.1 RLU. Conclusion Women initiating ARVs have a high prevalence of abnormal Pap smears and HR-HPV. Our results underscore the need

  5. Value of methylene blue chromoendoscopy in detection of early gastric cancer and precancerous lesions%亚甲蓝色素内镜对早期胃癌及癌前病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    冯业全; 陈雪; 陈万宁; 杨清清; 刘倩

    2012-01-01

    Objective To investigate the value of methylene blue chromoendoscopy in the detection of early gastric cancer and precancerous lesions. Methods 194 outpatients and inpatients in our hospital from January 2010 to December 2011 were divided into the experimental group and the control group according to different endoscopic examinations. The experimental group(104 cases) was performed the methylene blue chromoendoscopy and the control group(90 cases) received the conventional gastroscopic examination. The detection rates of early gastric cancer and precancerous lesion were compared between the two kinds of endoscopy. Results The detection rate of glandular epithelial intestinal metaplasia, atypical hyperplasia and adenocarcinoma in the experimental group was significantly higher than that in the control group(P0. 05). Conclusion The methylene blue chromoendoscopy has high detection rate for early gastric cancer and precancerous lesion and is conducive to find and diagnose early gastric cancer and precancerous lesion with the advantages of simple method, safety and practicability.%目的 探讨色素内镜对早期胃癌及癌前病变的诊断价值.方法 将2010年1月至2011年12月该院消化内科门诊及住院患者194例,根据不同内镜检查分为实验组和对照组,104例行亚甲蓝色素内镜检查的为实验组,对照组90例行普通胃镜检查,比较亚甲蓝色素内镜和普通内镜的早期胃癌及癌前病变的检出率.结果 实验组腺上皮肠化、不典型增生及腺癌检出率明显高于对照组(P<0.05);而腺上皮增生和腺瘤检出率两组比较,差异无统计学意义(P>0.05).结论 亚甲蓝色素内镜对早期胃癌及癌前病变的检出率更高,有助于早期胃癌及癌前病变的发现及诊断,具有方法简单、安全、实用的特点.

  6. Expression and clinical significance of E-cadherin,β-catenin and E-cadherin-catenins complex in breast cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Zuofeng Zhang; Shuguang Yang; Gangping Wang

    2014-01-01

    Objective: The aim of our study was to observe the expressions and clinical Significance of E-cadherin, β-catenin and E-cadherin-catenins complex in breast cancer and precancerous lesions, and analyze the relationship between the ex-pressions and clinicopathological features in breast cancer. Methods: Immunhistochemical UltraSensitiveTM S-P method was employed to detect the expression of E-cadherin, β-catenin and E-cadherin-catenins complex in 128 cases of invasive ductal carcinomas, 89 cases of ductal carcinoma in situ and 57 cases of atypical ductal hyperplasia, 53 cases of usual ductal hyper-plasia breast tissues were selected as a control group. The express of E-cadherin, β-catenin and their relationship with mult biological parameters including histological grade, region lymph node metastasis, distant metastasis and recurrence on files were also assessed. Results: (1) The staining patterns character of E-cadherin, β-catenin and E-cadherin-catenins complex:In UDH breast tissues, E-cadherin and a-catenin were expressed on cellmembrane of ductal and acinic cells, showing cellular contour and border among cells. The abnormal expression of the three proteins occurred in breast invasive ductal carcinomas, ductal carcinoma in situ and atypical ductal hyperplasia tissues, showing cytoplasmic or nuclear staining, decrease and loss of cytomembrane staining. (2) The abnormal expression rates of E-cadherin, β-catenin and E-cadherin-catenins complex in invasive ductal carcinomas were 53.91%, 65.63% and 81.25%, which were significantly higher than that in ductal carcinoma in situ, atypical ductal hyperplasia, usual ductal hyperplasia tissues (P 0.05). (3) There was a significantly dif erence in the mean E-cadherin, β-catenin and E-cadherin-catenins complex frequency between estrogen receptor & progesterone receptor positive IDC group and negative group, epidermal growth factor receptor type 2 (HER2/neu) positive and negative groups, Ki-67 proliferation index ≤ 14

  7. 色素内镜在上消化道早期癌及癌前病变的诊断价值%Diagnostic value for early cancer and precancerous lesions in the digestive tract under pigment endoscopy

    Institute of Scientific and Technical Information of China (English)

    陈函清; 潘文胜; 金冠福; 潘邦兴

    2012-01-01

    目的 研究色素内镜对上消化道早期癌及癌前病变的诊断价值.方法 将内镜下216例食管黏膜病变患者分为两组,染色组108例用Lugol氏液对食管黏膜染色,并对不染色和浅染色区进行病理活检;对照组108例食管黏膜患者进行单纯病理活检.将内镜下220例胃黏膜病变患者分为两组,染色组110例用靛胭脂一美兰染色后进行病理活检,对照组110例胃黏膜病变患者进行单纯病理活检.结果 食管染色组不染色或浅染色病例60例(55.6%),病理活检发现早期食管癌12例(11.1%),癌前病变6例(5.6%);胃染色组有69例(62.7%)不同程度染色,病理活检发现早期胃癌14例(12.7%),其中原位癌4例(3.6%),癌前病变9例(8.1%).发现早期癌及癌前病变与对照组的诊断率比较差异有显著性(P<0.01).结论 色素内镜有助于提高上消化道早期癌及癌前病变的诊断率及病理活检准确率,方法简便安全,值得基层医院推广.%[Objective] To study diagnostic value of early cancer and precancerous lesions in upper gastrointestinal under pigment endoscopy. [Methods] 216 cases of endoscopic esophageal mucosal lesions, staining group of 108 cases were treated with Lugol's solution staining of the esophageal mucosa, and non-staining and light staining zone biopsy; Control group, 108 cases of esophageal mucosa in patients with a simple biopsy. 220 cases of endo- scopic gastric mucosal lesions, indigo dyeing group of 110 cases with rouge - methylene blue staining and biopsy in the control group of 110 cases gastric mucosal lesions in patients with a simple biopsy. [Results] Esophagus didn't stain or stained pale group of 60 cases (55.6%), biopsy of 12 early esophageal cancer (11.1%), precancerous lesions in 6 patients (5.6%); Stomach stained group of 69 patients (62.7%) varying degrees of staining, biopsy detection of 14 early gastric cancer (12.7%), which carcinoma in situ in 4 cases, 9 cases of precancerous

  8. TCT联合阴道镜宫颈活检和LEEP诊治宫颈病变200例临床分析%Clinical analysis on 200 cases with cervical lesions diagnosed and treated by TCT combined with cervical biopsy under colposcope and LEEP

    Institute of Scientific and Technical Information of China (English)

    张敦兰; 雷燕; 周丽敏

    2011-01-01

    目的:探讨TCT联合阴道镜检查诊断官颈病变的准确率和LEEP术诊治官颈病变的效果.方法:对TCT联合阴道镜宫颈活检初步诊断为官颈疾病者200例采用LEEP术治疗,切除组织行病理检查.结果:TCT联合阴道镜官颈活检结果与LEEP术后病理结果的诊断符合率为97.00%,两者无显著性差异;LEEP术并发症发生率为8.9%.结论:TCT联合阴道镜宫颈活检与LEEP术后病理诊断无显著性差异,是宫颈上皮内瘤变及癌前病变筛查的较好方法;LEEP术是一种较好的确诊和治疗官颈病变的方法.%Objective: To explore the accurate rate of TCT combined with colposcopy for diagnosis of cervical lesions and the effi-cacy of loop electrosurgical excision procedure (LEEP) for diagnosis and treatment of cervical lesions. Methods; 200 cases who were diag-nosed as cervical lesions by TCT combined with cervical biopsy under colposcope were treated with LEEP, then the excised tissue samples were collected for pathological examination. Results: The diagnostic accordance rate of TCT combined with cervical biopsy under colposcope and pathological examination after LEEP was 97.00% , there was no significant difference between the two methods; the incidence of compli-cations after LEEP was 8. 9%. Conclusion: There is no significant difference in the accurate rate for diagnosis of cervical lesions between TCT combined with cervical biopsy under colposcope and pathological examination after LEEP, TCT combined with cervical biopsy under col-poscope is a good method to screen cervical intraepithelial neoplasia and precancerous lesion of cervix; LEEP is a good method for definite di-agnosis and treatment of cervical lesions.

  9. Location and Density of Immune Cells in Precursor Lesions and Cervical Cancer.

    Science.gov (United States)

    Bedoya, Astrid M; Jaramillo, Roberto; Baena, Armando; Castaño, Jorge; Olaya, Natalia; Zea, Arnold H; Herrero, Rolando; Sanchez, Gloria I

    2013-04-01

    Only a small proportion of women infected with Human Papillomavirus (HPV) develop cervical cancer. Host immune response seems to play a role eliminating the viral infection and preventing progression to cancer. Characterization of tumor infiltrating lymphocytes (TILs) in cervical pre-neoplastic lesions and cervical cancer may be helpful to understand the mechanisms that mediate this protection. The aim of this study was to determine if there are differences in the localization and density (cells/mm(2)) of CD8+ T-cells, CD4+ T-cells and Tregs (CD25 + Foxp3+) in cervical pre-neoplastic lesions and cervical cancer. Immunohistochemical analysis of sections of 96 (26 CIN1, 21 CIN2, 25 CIN3, and 24 SCC) samples revealed that regardless of CIN grades, CD8+ T-cells are more abundant than CD4+, CD25+ and Foxp3+ cells in both the stroma and epithelium. There was a higher density of CD8+ cells in the stroma of cervical cancer compared to CIN3 (OR = 4.20, 95% CI 1.2-15), CIN2 (OR = 7.86, 95% CI 1.7-36.4) and CIN1 (OR = 4.25, 95% CI 1.1-17). Studies evaluating whether these cells are recruited before or after cancer progression will be helpful to understand the role of these cells in the natural history of HPV-induced lesions.

  10. Endoscopic Submucosal Dissection for Early Esophageal Cancer and Esophageal Precancerous Lesions%内镜黏膜下剥离术治疗早期食管癌及食管癌前病变

    Institute of Scientific and Technical Information of China (English)

    薛鹏星; 周旋光; 陈娟

    2012-01-01

    Objective The efficacy of endoscopic submucosal dissection (ESD) in the treatment of early esophageal cancer and esophageal precancerous lesions was studied. Methods 4 patients with early esophageal cancer and 21 patients with esophageal precancerous lesions were treated by ESD after confirming the lesion range and invasion depth. Endoscopic ultrasonography was used to measure the invasion depth and lugol's staining was applied in measuring lesion range. Intraoperative mapping, submucosal injection, marginal mucosa incision, split and wound treatment were utilized as well. Results All lesions were completely removed, and no perforation or bleeding occurred during the operation. In the third month one patient was found to have esophageal stenosis which was relieved after a dilation. To random visit the patients 3, 6 and 12 months after operation, there was no recurrence. Conclusion ESD is a safe way to treat early esophageal cancer and esophageal precancerous lesions.%研究内镜黏膜下剥离术(ESD)治疗早期食管癌及食管癌前病变的治疗价值。对25例早期食管癌及食管癌前病变在确定病变范围及浸润深度后行黏膜下剥离术。先超声内镜判断浸润深度及利用Lugol液染色确定病灶范围,后采用术中标记、粘膜下注射、边缘粘膜切开、剥离及创面处理等方法。结果显示,病灶完整切除,术中无穿孔、出血;术后3、6、12月常规随访,均无复发。1例术后3个月出现食管狭窄,给予扩张后狭窄缓解。结果表明,对早期食管癌及食管癌前病变,内镜黏膜下剥离术是一种安全的内镜下治疗方法。

  11. Research progress in relationship between HPV testing and diagnosis and treatment of cervical lesions%HPV检测在宫颈疾病诊治中的研究进展

    Institute of Scientific and Technical Information of China (English)

    景笑笑; 杨雪峰

    2012-01-01

    Cervical cancer is the most common malignant tumor in female reproductive system, and its etiology is not entirely clear. Currently it is accepted that cervical cancer is related with human papilloma virus ( HPV ) infection. The persistent high - risk HPV infection is the main cause of cervical lesions. The detecting and therapy of high-risk HPV has become an important means in screening precancerous cervical diseases and preventing cervical cancer. This article summarized the relationship between HPV infection and cervical lesions, relationship between HPV viral load and classification and severity of cervical disease, the clinical value of HPV in evaluating therapeutic efficacy, judging prognosis and guiding cytology positive patients in shunt aspects and HPV vaccine.%宫颈癌是女性生殖系统最常见的恶性肿瘤,其发病原因尚未完全清楚,目前公认宫颈癌的发病与人乳头瘤病毒(HPV)感染有关,持续的高危型HPV感染是引起宫颈病变的主要病因,对高危型HPV的检测和治疗已成为筛查宫颈癌前病变、预防宫颈癌的重要手段.该文围绕HPV感染与宫颈病变的关系,HPV病毒载量、分型与宫颈病变严重程度的关系以及其在评估治疗效果、判断预后、指导细胞学阳性患者分流等方面的临床价值,HPV疫苗等的研究进展作以综述.

  12. Chromoendoscopy(Lugol's Solution) for Diagnosis of Early Esophageal Cancer and Precancerous Lesions%胃镜下卢戈液染色对早期食管癌及癌前病变的诊断

    Institute of Scientific and Technical Information of China (English)

    李小华; 罗忠金; 方慧祺; 何洁; 邓燕

    2011-01-01

    目的 探讨胃镜下卢戈液染色诊断早期食管癌及癌前病变的价值.方法 对40例胃镜下食管有可疑病变者行卢戈液染色,观察着色情况并取材常规送病理检查.结果 40例卢戈液染色有24例(36处)不着色区或淡染区,经病理诊断食管黏膜内癌2处,不典型增生20处,Barrett食管8处,单纯增生6处.结论 胃镜下卢戈液染色能提高早期诊断食管癌及癌前病变的水平.%Objective To discuss the value of chromoendoscopy (Lugol's solution) for the diagnosis of early esophageal cancer and precancerous lesions.Methods Chromoendoscopy with Lugol's solution was used in the esophagus to detect lesions in 40 patients with suspicious esophageal lesions, and biopsy specimens were taken from the lesions for pathologic diagnosis.Results Among the 40 cases,24 (36 regions) were not stained with Lugol' s solution.Pathologic examination showed esophageal mucous menbrane carcinoma in 2 cases, atypical hyperplasia in 20, Barrett's esophagus in 8 and simple hyperplasia in 6.Conclusion Chromoendoscopy with Lugol's solution can elevate diagnostic levels of early esophageal cancer and precancerous lesions.Therefore,it is worthy to be widely used in clinical practice.

  13. A probe on therapic experience on treating precancerous lesion of esophagus cancer with therapy of soothing liver and strengthening spleen%疏肝健脾益气法干预食道癌前病变的探讨

    Institute of Scientific and Technical Information of China (English)

    徐蕾

    2015-01-01

    癌症发病率的上升及其给社会带来的危害致使人们对癌前病变的诊断与及早处理日益重视,临证发现早期采用中医药干预,对食道癌前病变可发挥有益作用,预防其向食道癌发展。本文对中医药疏肝健脾益气法辨证干预食道癌前病变进行了探讨,。%The raising incidence of cancer and its harm attract more attention to the diagnosis of precancerous lesion of esophagus cancer and treatment in good time. It’s believed that Chinese medicine could help to take precautions against the worsening of esophagus cancer. This passage tried to discuss therapic experience on treating precancerous lesion of esophagus cancer with therapy of soothing liver and strengthening spleen.

  14. Immunohistochemical expression of p16ink4a in inflammatory, preneoplastic and neoplastic cervical lesions

    Directory of Open Access Journals (Sweden)

    Gajanin Radoslav

    2015-01-01

    Full Text Available Introduction. High-risk human papilloma viruses play a main role in the development of cervical dysplasias and carcinomas. p16INK4a can be considered as a surrogate marker of active highrisk human papillomaviruses infection in dysplastic and neoplastic cells of the cervix. This study was aimed at determining the presence and level of p16INK4a expression in inflammatory, preneoplastic and neoplastic lesions of the cervix. Material and Methods. The study was performed on 109 samples of cervical biopsy. Cervical cancer was diagnosed in 36 patients, 34 patients had a preneoplastic change (dysplasia in stratified squamous cervix epithelium and a nonspecific inflammatory process was found in 39 patients. In all samples, immunohistochemical analysis using antibodies to p16INK4a was performed. Results. The expression of p16INK4a was verified in all cases of cervical cancer (100%, in 67.65% of dysplastic cervical lesions and in 38.5% of inflammatory lesions. A statistically highly significant difference was found in the presence and level of expression among neoplasic, dysplastic and inflammatory lesions of the cervix (χ² = 76.02, p < 0.001. The expression was more frequent and had a higher level in neoplastic and high grade dysplastic lesions compared to expression in inflammatory lesions and low grade dysplasias. Conclusion. The analysis of the presence of p16INK4a can differentiate non-neoplastic, high grade preneoplastic and neoplastic changes of the cervix. The use of p16INK4a in interpreting borderline lesions of the cervix can enable a rational therapeutic treatment of patients.

  15. Quantitative Detection of Screening for Cervical Lesions with ThinPrep Cytology Test

    Institute of Scientific and Technical Information of China (English)

    Hong-xin ZHANG; Yi-min SONG; Su-hong LI; Yu-hui YIN; Dong-ling GAO; Kui-sheng CHEN

    2010-01-01

    OBJECTIVE To investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology (ThinPrep Cytology Test, TCT) and The Bethesda System (TBS), also with computer image analysis. METHODS With application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted in atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells-cannot exclude HSIL (ASC-H), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma (SCC) with the mean optical density (MOD), average grey (AG), positive units (PU), and nucleus to cytoplasmic ratio (N:C). Differences between each group of cells were compared and analyzed statistically.RESULTS Apart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups, there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of nucleus to cytoplasm were highly statistically signifi cant. CONCLUSION Stereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become a new means of cytological assisted diagnosis.

  16. 喉癌前病变中医证候及四诊特征初步研究%Pilot Study on Chinese Medicine Pattern/Syndrome and the Characteristics of Four Diagnostic Methods for Laryngeal Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    卢标清; 李云英; 孙一帆; 周小军; 李少华; 陈子清; 郭亿莲; 伍映芳

    2012-01-01

    目的 通过临床调查探讨喉癌前病变的中医证候及四诊特点,为喉癌前病变的中医药防治及研究提供参考和思路.方法 采用流行病学横断面调查方法,对88例喉癌前病变、100例声带息肉及60例喉癌患者的中医证候及四诊资料进行采集分析.结果 在声带息肉→喉癌前病变→喉癌的病情进展过程中,中医证候演变特点:以痰瘀为主线,证候由单一向证候兼杂发展,最终出现痰瘀热毒、气阴两虚等多种证候因素兼杂的情况.舌象变化特点:随着病情的发展变化,舌象可由正常的淡红舌为主,向暗红舌、红绛舌、紫舌等发展;舌苔由淡白、白腻向薄黄、黄腻到黄老厚腻变化发展;舌边瘀斑瘀点及舌下静脉曲张的情况更普遍.结论 痰瘀是喉癌前病变的基本病因病机,贯穿于喉癌前病变发生发展的始终.%Objective To clinically investigate and explore the Chinese medicine pattern/syndrome and the characteristics of the four diagnostic methods of laryngeal precancerous lesions so as to provide the references and thinking approaches to the prevention,treatment as well as the research of laryngeal precancerous lesions with traditional Chinese medicine. Methods The cross - sectional investigation of epidemiology was adopted to collect and analyze the Chinese medicine pattern/syndrome and the characteristics of four diagnostic methods for 88 cases of laryngeal precancerous lesions, 100 cases of vocal cord polyp and 60 cases of laryngocarcinoma. Results In the sickness progression, vocal cord polyp → laryngeal precancerous lesions → laryngocarcinoma,the characteristics of the evolution of the Chinese medicine pattern/syndrome indicated: The phlegm stasis pattern/syndrome was essential and the disorders developed more and more complicated. Finally the mixed pattern/syndrome occurred,such as phlegm stasis mixed with heat toxin,and deficiency of qi and yin. The characteristics of tongue conditions

  17. 5-year clinical performance of resin composite versus resin modified glass ionomer restorative system in non-carious cervical lesions

    DEFF Research Database (Denmark)

    Franco, Eduardo Batista; Benetti, Ana Raquel; Ishikiriama, Sérgio Kiyoshi

    2006-01-01

    To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions.......To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions....

  18. Progression and regression of cervical pap test lesions in an urban AIDS clinic in the combined antiretroviral therapy era: a longitudinal, retrospective study.

    Science.gov (United States)

    Lofgren, Sarah M; Tadros, Talaat; Herring-Bailey, Gina; Birdsong, George; Mosunjac, Marina; Flowers, Lisa; Nguyen, Minh Ly

    2015-05-01

    Our objective was to evaluate the progression and regression of cervical dysplasia in human immunodeficiency virus (HIV)-positive women during the late antiretroviral era. Risk factors as well as outcomes after treatment of cancerous or precancerous lesions were examined. This is a longitudinal retrospective review of cervical Pap tests performed on HIV-infected women with an intact cervix between 2004 and 2011. Subjects needed over two Pap tests for at least 2 years of follow-up. Progression was defined as those who developed a squamous intraepithelial lesion (SIL), atypical glandular cells (AGC), had low-grade SIL (LSIL) followed by atypical squamous cells-cannot exclude high-grade SIL (ASC-H) or high-grade SIL (HSIL), or cancer. Regression was defined as an initial SIL with two or more subsequent normal Pap tests. Persistence was defined as having an SIL without progression or regression. High-risk human papillomavirus (HPV) testing started in 2006 on atypical squamous cells of undetermined significance (ASCUS) Pap tests. AGC at enrollment were excluded from progression analysis. Of 1,445 screened, 383 patients had over two Pap tests for a 2-year period. Of those, 309 had an intact cervix. The median age was 40 years and CD4+ cell count was 277 cells/mL. Four had AGC at enrollment. A quarter had persistently normal Pap tests, 64 (31%) regressed, and 50 (24%) progressed. Four developed cancer. The only risk factor associated with progression was CD4 count. In those with treated lesions, 24 (59%) had negative Pap tests at the end of follow-up. More studies are needed to evaluate follow-up strategies of LSIL patients, potentially combined with HPV testing. Guidelines for HIV-seropositive women who are in care, have improved CD4, and have persistently negative Pap tests could likely lengthen the follow-up interval.

  19. Prevalence of human papillomavirus DNA in female cervical lesions from Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    S. M. B. Cavalcanti

    1994-12-01

    Full Text Available A hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (HPV types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. The data were compared with histologic diagnosis. Eighty-eight (55 biopsies contained HPV DNA sequences. In low grade cervical intraepithelial neoplasias (CIN I, HPV infection was detected in 78.7 of the cases, the benign HPV 6 was the most prevalent type. HPV DNA was detected in 58 of CIN II and CIN III cases and in 41.8 of squamous cell carcinomas (SCC. Histologically normal women presented 20 of HPV infection. Oncogenic HPV was found in 10 of these cases, what may indicate a higher risk of developing CINs and cancer. Twenty-five percent of the infected tissues contained mixed infections. HPV 16 was the most common type infecting the cervix and its prevalence raised significantly with the severity of the lesions, pointing its role in cancer pathogenesis. White women presented twice the cervical lesions of mulatto and African origin women, although HPV infection rates were nearly the same for the three groups (approximately 50. Our results showed that HPV typing by in situ hybridization is a useful tool for distinguishing between low and high risk cervical lesions. Further studies are required to elucidate risk factors associated with HPV infection and progression to malignancy in Brazilian population.

  20. One-year clinical evaluation of tooth-coloured materials in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Stojanac Igor

    2011-01-01

    Full Text Available Introduction. The restoration of non-carious cervical lesions is specific, mostly because of the location of their margins, especially the cervical margin, which is determined in cement and/or dentine. This feature makes the cervical margin more susceptible to micro-leak­age, causing marginal discoloration, postoperative sensitivity, development of the secondary caries and loss of the restoration. Material and methods. One of the criteria for inclusion of a patient in this study was the presence of at least two non-carious cervical lesions to be restored with the minimum depth of 1mm, independently of their location in the dental arch. A total of 60 restorations were placed in 30 patients, and every patient received both tested materials (composite resins and compomer on their non-carious cervical lesions. The clinical evaluation of the therapeutic success was performed six months and then one year after the day of the placement of restorations using the modified-United States Public Health Service criteria. The following was evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. Results. A statistically significant high percent of restorations/teeth with postoperative sensitivity was found in the group of resin composite restorations after six months. At the end of the evaluation period, that is after one year, there were no statistically significant differences between materials for all evaluated criteria. Conclusion. The results of this study show the identical quality of both examined materials one year after the readjustment of non-carious cervical lesions.

  1. Percutaneous anterolateral balloon kyphoplasty for metastatic lytic lesions of the cervical spine.

    Science.gov (United States)

    Lykomitros, Vasilis; Anagnostidis, Kleovoulos S; Alzeer, Ziad; Kapetanos, George A

    2010-11-01

    The purpose of our report is to describe a new application of kyphoplasty, the percutaneous anterolateral balloon kyphoplasty that we performed in two cases of metastatic osteolytic lesions in cervical spine. The first patient, aged 48 years, with primary malignancy in lungs had two metastatic lesions in C2 and C6 vertebrae. Patient's complaints were about pain and restriction of movements (due to the pain) in the cervical spine. The second patient, aged 70 years, with primary malignancy in stomach, had multiple metastatic lesions in thoracolumbar spine and C3, C4 and C5 vertebrae without neurological symptoms. The main symptoms were from cervical spine with severe pain even in bed rest and systematic use of opiate-base analgesic. The preoperative status was evaluated with X-rays, CT scan, MRI scan and with Karnofsky score and visual analogue pain (VAS) scale. Both patients underwent percutaneous anterolateral balloon kyphoplasty via the anterolateral approach in cervical spine under general anaesthesia. No clinical complications occurred during or after the procedure. Both patients experienced pain relief immediately after balloon kyphoplasty and during the following days. The stiffness also resolved rapidly and cervical collars were removed. VAS score significantly improved from 85 and 95 preoperatively to 30 in both patients. Karnofsky score showed also improvement from 40 and 30 preoperatively to 80 and 70, respectively, at the final follow-up (7 months after the procedure). Fluoroscopy-guided percutaneous anterolateral balloon kyphoplasty proved to be safe and effective minimally invasive procedure for metastatic osteolytic lesions of the cervical spine, reducing pain and avoiding vertebral collapse. Experience and attention are necessary in order to avoid complications.

  2. Association between colposcopic findings and histology in cervical lesions: the significance of the size of the lesion.

    Science.gov (United States)

    Kierkegaard, O; Byralsen, C; Hansen, K C; Frandsen, K H; Frydenberg, M

    1995-04-01

    The relation between eight specific colposcopic diagnostic findings and the histologic grade of a cervical lesion in 896 women was evaluated. The size of the transformation zone (TZ), the size of the lesion, the intensity of the color tone, distinct margins, the pathology of the vessels, and the presence of micropapillae as single findings were highly statistically correlated to the histologic grade (P < 0.0001). By logistic regression analysis the risk for a higher histologic grade when assessed by colposcopy was greatest in women with variation of the acetowhite color (odds ratio (OR) = 16.0; 95% CI, 10.0-26.0) followed by coarse vessels (OR = 10.0; CI, 3.2-34.0). Lesion-size larger than 50% of the visible cervix had an OR of 3.6 (CI, 2.1-6.3). Extention beyond TZ had an OR of 0.4 (CI, 0.2-0.4) and larger TZ had an OR of 0.5 (CI, 0.3-0.9). In conclusion we found that the size of the cervical lesion had some independent predictive value and should be considered in future trials.

  3. Application of confocal laser endomicroscopy in diagnosis of digestive tract cancer and precancerous lesions%共聚焦激光显微内镜在消化道肿瘤及癌前病变中的应用

    Institute of Scientific and Technical Information of China (English)

    杨竞; 范楠楠; 杨云生

    2012-01-01

    Confocal laser endomicroscopy (CLE) is a novel and advanced endoscopic technique, which can clearly display the structures of the mucosal surface and subsurface during ongoing endoscopy, thereby providing immediate diagnosis of mucosal lesions. CLE can distinguish the physiological change from the pathological in the gastrointestinal mucosa by using a fluorescent contrast agent, which demonstrates its potential in diagnosis of gastrointestinal diseases. A series of studies have shown that CLE has high specificity and sensitivity in diagnosis and distinction of gastrointestinal tumors, especially the early cancer and precancerous lesions. The present paper will focus on advances in researches about CLE in gastrointestinal tumors and precancerous lesions.%共聚焦激光显微内镜(CLE)是一项在常规内镜检查同时能清晰地显示黏膜表面及表面下组织结构,从而对黏膜病变做出即时诊断的全新的内镜技术.CLE在荧光对比剂作用下能够对胃肠道黏膜的病理生理改变进行准确诊断,显示了其用于消化道疾病诊断的潜能.一系列研究表明CLE对消化道肿瘤尤其是早期肿瘤及癌前期病变的诊断及鉴别诊断具有较高的特异性和敏感性,本文就其在消化道肿瘤及癌前病变中的应用进展做一综述.

  4. The 836 Cases of Cervical Cancer Screening Lesions Result Analysis%宫颈癌前期病变筛查836例结果分析

    Institute of Scientific and Technical Information of China (English)

    杨惠新

    2012-01-01

    Objective:To summarize and analyze the results of precancerous lesion screening for cervical caner and provide evidence for cervical caner prevention.Methods:The characteristics such as age,education level,birth history and cervical laceration,episiotomy of the 836 participants were reviewed,the results of LCT and histopathologic diagnosis were compared,and the LCT results of foreign female labors and local females were also compared and analyzed.Results:The positive rate of LCT was 10.77%,the accuracy was 89.89%.The proportions of history of birth,episiotomy and cervical laceration in LCT positive participants were statistically significantly higher than in the LCT negative participants (P<0.05).Positive rate of LCT in foreign female labors was statistically higher than in local females (P<0.05). Conclusion:LCT is a ideal screening tool for cervical cancer in this area,gynecologic health care education should be enhanced in foreign female labors to improve the activity and compliance for participating the cervical cancer screening.%目的:总结分析宫颈癌前期病变LCT筛查结果,为本地区宫颈癌预防提供依据.方法:回顾调查836例参加宫颈癌前期病变LCT筛查妇女的年龄、文化程度、分娩史和宫颈撕裂、会阴切除术等病史,对比分析LCT检查及其与组织病理学诊断结果,对比分析外来务工妇女与本地妇女参加检查情况和检查结果.结果:LCT阳性率为10.77%,准确率为89.89%.LCT阳性者,有分娩、会阴切开术、宫颈撕裂史比例高于LCT阴性者,差异具有统计学意义(P<0.05).外来务工妇女LCT检出率高于本地居民,差异具有统计学意义(P<0.05).结论:LCT是本地区较为理想的宫颈癌筛查方法;应大力加强外来务工妇女妇科保健宣传力度,提高其参加宫颈癌筛查的主动性和依从性.

  5. 中医药治疗慢性萎缩性胃炎癌前病变研究述评%Research Review of Traditional Chinese Medicine in the Treatment of Precancerous Lesion of Chronic Atrophic Gastritis

    Institute of Scientific and Technical Information of China (English)

    莫喜晶; 韦春回

    2013-01-01

    目的:探讨中医药治疗慢性萎缩性胃炎癌前病变现状,指导临床用药.方法:总结、归纳和分析近年来的相关文献,从病因病机、辨证论治、中医专方、中西医结合及中医外治疗法等几个方面对癌前病变研究现状进行详尽的阐述.结果:中医药无论是在辨证用药、专方专治还是在中西医结合及外治方面,治疗慢性萎缩性胃炎癌前病变的临床研究均取得了可喜的成果,突显了中医药治疗萎缩性胃炎的独特优势.结论:中医药治疗慢性萎缩性胃炎疗效肯定,具有广阔的前景,但在临床研究中尚需建立统一规范的辨证标准和量化指标,采用循证医学的方法进行科研设计.%Objective:To discuss the current situation of traditional Chinese medicine in the treatment of precancerous lesion of chronic atrophic gastritis and to guide the clinical medication. Methods:The related literatures were concluded,summarized,and analyzed. The research status of precancerous lesion was expounded in details from several aspects of the etiology and pathogenesis, syndrome differentiation and treatment,TCM special medicine,combination of traditional Chinese and western medicine, and TCM external therapy. Results; Traditional Chinese medicine whether in the aspects of medicine usage according to syndrome differentiation, special medicine and treatment or combination of traditional Chinese and western medicine,external treatment,the clinical studies of treatment for precancerous lesion of chronic atrophic gastritis made great achievements, which highlighted unique advantages of Chinese medicine in the treatment of atrophic gastritis. Conclusion: Traditional Chinese medicine in the treatment of chronic atrophic gastritis has confirmed therapeutic effectiveness, and good prospects. While unified and standardized syndrome differentiation standard and quantitative index are still needed to establish in the clinical study and the method of evidence

  6. 皮肤恶性肿瘤及癌前病变临床病理探讨%The study of the clinical pathological on skin cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    李伟霞

    2014-01-01

    Objective:To investigate the clinical features and pathogenesis of skin cancer and precancerous lesions.Methods:100 patients with skin cancer were selected from May 2010 to May 2014.We retrospectively analyzed their clinical data,and observed the clinical features and pathogenesis of skin cancer and precancerous lesions.Results:Basal cell carcinoma,actinic keratosis and Bowen's disease were the main types of skin malignant tumor;the predilection site of basal cell carcinoma always in human face, and Bowen's disease were common in human torso;the highest coincidence rate of clinical diagnosis and pathological diagnosis of malignant skin tumors were paget disease,malignant melanoma and squamous cell carcinoma.Conclusion:Analysis of the clinical features and pathogenesis of skin cancer and precancerous lesions provides important reference for the clinical treatment,to improve the cure rate.%目的:探讨皮肤恶性肿瘤及癌前病变的发病机制和临床特征。方法:2010年5月-2014年5月收治皮肤恶性肿瘤患者100例,对其临床资料进行回顾性分析,观察皮肤恶性肿瘤及癌前病变的发病机理和临床特征。结果:基底细胞癌、日光性角化、鲍温病是皮肤恶性肿瘤的主要类型;基底细胞癌多发于人体面部,鲍温病多发于人体躯干部位;皮肤恶性肿瘤临床诊断与病理诊断的符合率最高的是Paget病、恶性黑素瘤、鳞状细胞癌。结论:对皮肤恶性肿瘤及癌前病变的发病机制和临床特征进行有效分析,为临床治疗提供重要的参考资料,提高了治愈率。

  7. Implication of HLA-C and KIR alleles in human papillomavirus infection and associated cervical lesions.

    Science.gov (United States)

    Rizzo, Roberta; Gentili, Valentina; Rotola, Antonella; Bortolotti, Daria; Cassai, Enzo; Di Luca, Dario

    2014-11-01

    Human papillomavirus (HPV) regulation of host immune response leads to cervical lesions. In particular, natural killer (NK) cells are crucial for HPV control. Since specific HLA-I/KIR interactions modify NK cell activation, we analyzed HLA-C and KIR alleles in HPV infection and lesion development in 150 controls, 33 condyloma acuminatum, and 111 invasive cervical cancer (ICC) patients. We showed an increase in HLA-C1/KIR2DL2 and HLA-C1/KIR2DL3 pairs in HPV high-risk infected patients (OR 3.05, 3.24) with ICC (OR 1.33, 3.68). These data suggest HLA-C and KIR typing as risk marker for HPV infection and lesion evolution.

  8. Management of precancerous conditions and lesions in the stomach (MAPS): guideline from the European Society of Gastrointestinal Endoscopy (ESGE), European Helicobacter Study Group (EHSG), European Society of Pathology (ESP), and the Sociedade Portuguesa de Endoscopia Digestiva (SPED).

    Science.gov (United States)

    Dinis-Ribeiro, M; Areia, M; de Vries, A C; Marcos-Pinto, R; Monteiro-Soares, M; O'Connor, A; Pereira, C; Pimentel-Nunes, P; Correia, R; Ensari, A; Dumonceau, J M; Machado, J C; Macedo, G; Malfertheiner, P; Matysiak-Budnik, T; Megraud, F; Miki, K; O'Morain, C; Peek, R M; Ponchon, T; Ristimaki, A; Rembacken, B; Carneiro, F; Kuipers, E J

    2012-01-01

    Atrophic gastritis, intestinal metaplasia, and epithelial dysplasia of the stomach are common and are associated with an increased risk for gastric cancer. In the absence of guidelines, there is wide disparity in the management of patients with these premalignant conditions. The European Society of Gastrointestinal Endoscopy (ESGE), the European Helicobacter Study Group (EHSG), the European Society of Pathology (ESP) and the Sociedade Portuguesa de Endoscopia Digestiva (SPED) have therefore combined efforts to develop evidence-based guidelines on the management of patients with precancerous conditions and lesions of the stomach (termed MAPS). A multidisciplinary group of 63 experts from 24 countries developed these recommendations by means of repeat online voting and a meeting in June 2011 in Porto, Portugal. The recommendations emphasize the increased cancer risk in patients with gastric atrophy and metaplasia, and the need for adequate staging in the case of high grade dysplasia, and they focus on treatment and surveillance indications and methods.

  9. Skin cancer and precancerous skin lesions:clinical analysis of 632 cases%632例皮肤恶性肿瘤及癌前病变临床分析

    Institute of Scientific and Technical Information of China (English)

    黄远深; 李航; 涂平; 陈喜雪; 杨淑霞; 武玲慎; 赵俊郁; 李雪迎

    2010-01-01

    目的 探讨皮肤恶性肿瘤的临床特点.方法 回顾2005-2008年病理诊断皮肤恶性肿瘤及癌前病变的病例资料,重新阅片确认肿瘤诊断和病理亚型分型后,利用统计软件进行分析.结果 基底细胞癌及原位和侵袭性鳞状细胞癌最多见,分别占总病例数的29.3%及24.2%.在所研究病例中,>60岁发病者占55.4%,35~59岁发病比例达到34.3%,青少年患者比例为10.3%.Paget病临床诊断与病理诊断的符合率接近90.0%,但其他常见皮肤恶性肿瘤及癌前病变临床与病理的符合率仅在70.0%左右.结论 在皮肤恶性肿瘤及癌前病变中,头面部皮肤肿瘤及癌前病变病例明显多于其他部位.中青年患者不仅在皮肤淋巴瘤相关肿瘤病例中多见,而且在上皮来源的皮肤恶性肿瘤及黑素瘤病例中占有显著比例.%Objective To investigate the clinical features of skin cancer.Methods Clinical data of skin cancer and precancerous skin lesions confirmed pathologically from 2005 to 2008 in Peking University First Hospital were retrospectively analyzed by using statistical methods.Results A total of 632 cases of skin cancer and precancerous skin lesions were studied.The most common skin cancer was basal cell carcinoma and squamous cell carcinoma (invasive and in situ) which accounted for 29.3%and 24.2%,respectively.The average age at onset was older than 60 years in 55.4%of the patients,between 35 and 59 years in 34.3%,younger than 35 years in 10.3%.The concordance between clinical and pathological diagnosis reached nearly 90.O%for Paget's disease,70.0% for other common skin cancer and precancerous skin lesions.Conclusions Skin cancer and precancerous skin lesions have a predilection for scalp and face.Patients aged from 35 to 59 years account for a significant proportion not only in cutaneous lymphoma but also in melanoma and epithelium-derived nonmelanoma skin cancer.

  10. 内镜色素染色对早期胃癌及癌前病变的诊断价值研究%The value of methylene blue staining in diagnosis of precancerous lesions and early gastric cancer

    Institute of Scientific and Technical Information of China (English)

    何东凌; 冯青

    2009-01-01

    Objective To evaluate the value of methylene blue staining in diagnosis of precancerous lesions and early gastric cancer.Methods There are 224 paients with abnormal gastric mucous membrance,104 cases were biopsy after stain by Methylene blue and 120 cases were taken biopsy simplicity.Results One hundred and four suspected early careinonm of stomach cases were stain by Methylene blue and 81 eases were found dyeing.There are 75 patients with atypical hyperplasia or intestinal metaplasi,6 with early gastric cancers,4 with preinvasive carcinoma.One hundred and twenty patients were taken biopsy simplicity.There are 14 patients with mild to moderate atypical hyperplasia,36 cases of chronic gastritis with focal intestinal metaplasia.Conclusions The early diagnosis rate of precancerous lesions and early gastric cancer are very well improved by methylene blue staining.%目的 评价和探讨内镜色素染色对早期胃癌及癌前病变的诊断价值.方法 对224例胃镜下粘膜异常表现患者,104例局部美蓝染色后活检,120例单纯活检.结果 美蓝染色组104例,有81例有不同程度染色,病理示不典型增生及肠上皮化生75例,早期胃癌6例,其中原位癌4例.单纯活检组120例,14例存在轻度至中度不典型增生,36例慢性胃炎伴肠化局灶性肠化.结论 内镜色素染色有助于提高早期胃癌及癌前病变的早期诊断.

  11. Clinical Observation on Replenishing Spleen Qi and Nourishing Yin Method of Gastric Precancerous Lesions%益气健脾养阴法治疗胃癌前病变临床观察

    Institute of Scientific and Technical Information of China (English)

    谢晶日; 李柏

    2013-01-01

    目的:观察研究益气健脾养阴法治疗胃癌前病变的临床疗效.方法:将48例患者随机分为治疗组24例和对照组24例.治疗组给予益气健脾养阴方,每日1剂;对照组给予胃复春片,每日3次,每次4片,饭前空腹服用.3个月为1个疗程,均治疗2个疗程.结果:治疗后治疗组的临床症状、胃镜及病理学表现均明显好于对照组(P<0.05).结论:益气健脾养阴法治疗胃癌前病变有显著疗效.%Objective:To observe and study the curative effect of replenishing spleen qi and nourishing Yin method of gastric precancerous lesions.Methods:48 patients were randomly divided into treatment group (24 cases) and control group (24 cases).The treatment group was given the replenishing spleen qi and nourishing Yin therapy once a day.The control group was given Weifuchun Tablet three times a day and four tables for once.Three months was for a period of treatment.There were two treatment courses.Results:After treatment,the clinical symptoms,gastroscope and pathology performance of treatment group were significantly better than those of the control group (P < 0.05).Conclusion:The replenishing spleen qi and nourishing Yin method treatment of gastric precancerous lesion has remarkable curative effect.

  12. Distribution of HPV Genotypes and Involvement of Risk Factors in Cervical Lesions and Invasive Cervical Cancer: A Study in an Indian Population

    Science.gov (United States)

    Srivastava, Shikha; Shahi, U P; Dibya, Arti; Gupta, Sadhana; Roy, Jagat K

    2014-01-01

    Human papilloma virus (HPV) is considered as the main sexually transmitted etiological agent for the cause and progression of preneoplastic cervical lesions to cervical cancer. This study is discussing the prevalence of HPV and its genotypes in cervical lesions and invasive cervical cancer tissues and their association with various risk factors in women from Varanasi and its adjoining areas in India. A total of 122 cervical biopsy samples were collected from SS Hospital and Indian Railways Cancer Institute and Research Centre, Varanasi and were screened for HPV infection by PCR using primers from L1 consensus region of the viral genome. HPV positive samples were genotyped by type-specific PCR and sequencing. The association of different risk factors with HPV infection in various grades of cervical lesion was evaluated by chi-square test. A total of 10 different HPV genotypes were observed in women with cervicitis, CIN, invasive squamous cell cervical carcinoma and adenocarcinoma. Increased frequency of HPV infection with increasing lesion grade (p=0.002) was observed. HPV16 being the predominant type was found significantly associated with severity of the disease (p=0.03). Various socio- demographic factors other than HPV including high parity (p<0.0001), rural residential area (p<0.0001), elder age (p<0.0001), low socio-economic status (p<0.0001) and women in postmenopausal group (p<0.0001) were also observed to be associated with cervical cancer.These findings show HPV as a direct cause of cervical cancer suggesting urgent need of screening programs and HPV vaccination in women with low socio-economic status and those residing in rural areas. PMID:25035855

  13. Extracranial Vertebral Artery Aneurysm Presenting as a Chronic Cervical Mass Lesion

    Directory of Open Access Journals (Sweden)

    Lampis C. Stavrinou

    2010-01-01

    Full Text Available Background. Aneurysms of the extracranial vertebral artery are rare and can provide a diagnostic and therapeutic challenge. Methods. We reviewed the clinical history of a patient presenting with cervical radiculopathy, who harboured an extracranial vertebral artery aneurysm eroding the cervical spine. Results. CT Angiography and MR Angiography set the diagnosis, by revealing a left C5-C6 vertebral artery aneurysm with cervical root impingement. Bony reconstruction depicted enlargement of the C6 transverse foramen and a marked enlargement of the C6-C7 intravertebral foramen. The lesion was treated by intravascular proximal vertebral artery occlusion. Conclusions. Extracranial vertebral artery aneurysms require a high index of clinical suspicion. This is the first report of a vertebral artery pseudoaneurysm presenting with bony erosion, which supports a less minacious portrayal of vertebral artery aneurysms.

  14. Human papillomavirus infection and cervical lesions in rheumatic diseases: a systematic review.

    Directory of Open Access Journals (Sweden)

    Ana Raposo

    2016-07-01

    Full Text Available An association between immune-mediated diseases and cervical pre-malignant and malignant lesions is described, having the human papillomavirus (HPV infection a causal role. Related studies have been generally focused on systemic lupus erythematosus (SLE patients, but relatively to other diseases, such as rheumatoid arthritis (RA, Sjögren's syndrome (SS and systemic sclerosis (SSc, data has not been systematically evaluated. We conducted a systematic review analysis of the literature in PubMed, including articles published until March of 2015, in patients with RA, SS, SLE and SSc, to evaluate the frequency of HPV infection, cervical dysplasia and cervical cancer, and associated factors, with particular interest on the role of glucocorticoids and immunosuppressive treatment. Moreover, safety and efficacy of HPV vaccines in these patients was investigated. Of 476 articles identified, 27 were finally included. The studies showed an increased prevalence of cervical dysplasia and cancer, with the HPV infection being an important associated factor, in particular in SLE patients. The data relatively to other rheumatic diseases was very scarse, but an increased prevalence of smear abnormalities was also found in RA. Patients exposed to glucocorticoids and to long-term immunosuppression, particularly cyclophosphamide, have increased risk of presenting more pre-malignant lesions than the general population. The available vaccines seem to be generally safe and immunogenic in the short- period evaluation, but long-term follow-up is required to evaluate the impact of the vaccine in the protection against HPV infection and occurrence of high-grade cervical lesions.

  15. Assessment of quality of life for the patients with cervical cancer at different clinical stages

    Institute of Scientific and Technical Information of China (English)

    Yao Xie; Fang-Hui Zhao; Si-Han Lu; He Huang; Xiong-Fei Pan; Chun-Xia Yang; You-Lin Qiao

    2013-01-01

    With improved overall survival of cervical cancer patients,the importance of the quality of life (QOL)is increasingly recognized.This study was conducted to compare the QOL of women with different stage cervical cancer before and after treatment to facilitate improved cervical cancer prevention and treatment.We used the generic Medical Outcomes Study Short Form-36 (MOS SF-36) to collect QOL information.Based on SF-36,we interviewed cervical cancer patients at West China Second Affiliated Hospital and Sichuan Cancer Hospital between May 2010 and January 2011.A total of 92 patients with precancerous lesions,93 with early cancer,and 35 with advanced cancer responded to our survey.Average physical component summary (PCS) scores were significantly different between the three groups at every time point (P < 0.05).Average mental component summary (MCS) scores were significantly different between the three groups after treatment (P < 0.05).Average PCS and MCS scores increased gradually from the pretreatment to posttreatment period for patients with precancerous lesions.However,they reached the lowest at 1 month after treatment for patients with early and advanced cancers and rebounded between 1and 6 months after treatment.Our results indicate that patients with precancerous lesions and early cervical cancer show better overall QOL than do those with advanced cervical cancer.Additionally,patients with early cancer recover more quickly than do those with advanced cancer in terms of both physical and mental functions.Thus,early detection and treatment initiatives may improve the QOL for patients with precancerous lesions and cervical cancer.

  16. Cervical cancer: screening, diagnosis and staging.

    Science.gov (United States)

    Tsikouras, Panagiotis; Zervoudis, Stefanos; Manav, Bachar; Tomara, Eirini; Iatrakis, George; Romanidis, Constantinos; Bothou, Anastasia; Galazios, George

    2016-01-01

    Purpose: Despite the widespread screening programs, cervical cancer remains the third most common cancer in developing countries. Based on the implementation of cervical screening programs with the referred adoption of improved screening methods in cervical cytology with the knowledge of the important role of the human papilloma virus (HPV) it's incidence is decreased in the developed world. Even if cervical HPV infection is incredibly common, cervical cancer is relatively rare. Depending on the rarity of invasive disease and the improvement of detection of pre-cancerous lesions due to the participation in screening programs, the goal of screening is to detect the cervical lesions early in order to be treated before cancer is developed. In populations with many preventive screening programs, a decrease in cervical cancer mortality of 50-75% is mentioned over the past 50 years. The preventive examination of vagina and cervix smear, Pap test, and the HPV DNA test are remarkable diagnostic tools according to the American Cancer Association guidelines, in the investigation of asymptomatic women and in the follow up of women after the treatment of pre-invasive cervical cancer. The treatment of cervical cancer is based on the FIGO 2009 cervical cancer staging.

  17. Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions

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    Vallejo-Ruiz Verónica

    2010-11-01

    Full Text Available Abstract Background Altered sialylation has been observed during oncogenic transformation. Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumor progression and metastases. In the cervical cancer high levels of sialic acid have been reported in the patients serum, and an increased of total sialic acid concentration has been reported for the cervical neoplasia and cervical cancer. This study investigates the changes in expression and distribution of α2,3-linked sialic acid and α2,6- linked sialic acid in low and high squamous intraepithelial lesions and in normal tissue. Methods Lectin histochemistry was used to examine the expression and distribution of sialic acid in different grades of cervical neoplasia. We applied Maackia amurensis lectin, which interacts with α2,3-linked sialic acid and Sambucus nigra lectin specific for α2,6-linked sialic acid. Results The histochemical analysis showed that α2,3-linked sialic acid and α2,6- linked sialic acid increased in intensity and distribution in concordance with the grade of squamous intraepithelial lesion (SIL. These results are in concordance with a previous study that reports increased RNAm levels of three sialyltransferases. Conclusions These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. These findings provide the basis for more detailed studies of the possible role of cell surface glycoconjugates bearing sialic acid in the cellular cervix transformation.

  18. Human Papillomavirus Types 52 and 58 Are Prevalent in Uterine Cervical Squamous Lesions from Japanese Women

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    Kazuhiro Takehara

    2011-01-01

    Full Text Available Objective. To estimate the prevalence and genotypes of high-risk human papillomavirus (HPV focusing HPV 16, 18, 52, and 58 in Japan. Methods. Liquid-base cytology specimens were collected from Japanese women (n=11022, aged 14–98. After classifying cytodiagnosis, specimens were analyzed for HPV DNA by the multiplex polymerase chain reaction method, where 1195 specimens were positive for cervical smear, except adenomatous lesions. Result. HPV genotypes were detected in 9.5% of NILM and 72.2% of ASC-US or more cervical lesions. In positive cervical smears, HPV genotypes were HPV 52 at 26.6%, HPV 16 at 25.2%, HPV 58 at 21.8%, and HPV 18 at 7.1%. Most patients infected with HPV 16 were between 20–29 years old, decreasing with age thereafter. As for HPV 52 and 58, although the detection rate was high in 30- to 39-year-olds, it also was significant in the 50s and 60s age groups. Conclusion. In Japan, as a cause of abnormal cervical cytology, HPV52 and 58 are detected frequently in addition to HPV 16. In older age groups, HPV 52 and 58 detection rates were higher than that observed for HPV 16. After widespread current HPV vaccination, we still must be aware of HPV 52 and 58 infections.

  19. Characterization of High Grade Intraepithelial Cervical Lesion among Adolescents and Young Women

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    Carlos Eric Daudinot Cos

    2011-11-01

    Full Text Available Introduction: considering the relationship between human papilomavirus and cervical cancer, the current increase in sexually transmitted diseases constitutes the biggest threaten to adolescents’ health. Objective: to characterize high-degree intraepithelial cervical lesion in adolescents and young women. Method: a descriptive and observational study was conducted. It included 52 patients, all of them younger than 24 years old, who had been diagnosed with high-degree cervical pathology and were treated in the cervix pathology consultation of the "Ramón González Coro" Teaching Gynecologic and Obstetric Hospital from January 2007 to December 2008. Risk factors and diagnosis variables were studied and processed using the SPSS 11.5 system for Windows, through the determination of absolute and relative frequencies. Results: 78,8 % of patients had from 20 to 24 years old, 65,3 % of them began having sexual relationships between 15 and 17 years old, 62,2 % had had 3 or more sexual partners and 67,3 % used no contraceptive method. Cyto- histological correlation was of 100 % for intraepithelial cervical lesion II and III. Conclusion: the results of the present study compromise us to highlight the need of extended sexual education among young people.

  20. Osteosíntesis cervical posterior con placa en lesiones cervicales

    OpenAIRE

    2002-01-01

    El propósito del presente estudio es analizar retrospectivamente los resultados a largo plazo de las lesiones de la columna cervical baja tratadas mediante estabilización posterior con placas atornilladas de Roy-Cmille a las masas laterales. Pacientes y métodos: Se estudian 15 pacientes, de los cuales sólo 14 completos con lesiones traumáticas (13) y 1 no traumática (1 paciente con inestabilidad sobre artrosis por doble Cloward) durante un periodo postoperatorio medio de 8 años (6-12). Salvo ...

  1. The Application Value of Colposcopy Combined with Cervical Cytology in Cervical Lesions%阴道镜检查结合宫颈细胞学在宫颈病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王少华

    2014-01-01

    目的:探讨阴道镜检查结合宫颈细胞学在宫颈病变诊治中的临床应用价值。方法随机选在该院接收的656例行阴道镜检查结合宫颈细胞学的妇女作为研究对象,对所有对象的资料进行回顾性分析,总结患者的治疗效果。结果单纯行阴道镜检查筛查3例,无漏诊,癌前期可疑1例。单纯行新柏氏液基细胞学检测检查筛选宫颈癌4例,漏诊1例。行阴道镜检查结合新柏氏液基细胞学检测符合宫颈癌病例5例,无漏诊。结论单纯性阴道镜检查或者新柏氏液基细胞学检测宫颈癌存在漏诊情况,而结合两种方法进行检查后无漏诊现象,即结合两种方法诊断早期宫颈癌有助于提高疾病检出率,值得在临床上推广和使用。%Objective To investigate the clinical application value of colposcopy combined with cervical cytology in the diagnosis and treatment of cervical lesions. Methods 656 cases of women underwent colposcopy combined with cervical cytology in our hos-pital from March, 2012 to March, 2013 were randomly selected as the subjects, and the data of them were analyzed retrospectively, the treatment effect was summarized. Results A simple colposcopy screening 3 cases, no missed diagnosis, pre-cancerous suspi-cious 1 case. Simple ThinPrep liquid-based cervical cytology screening examination in 4 cases, missed 1 case. Colposcopy com-bined with ThinPrep liquid-based cervical cytology in line 5 cases, no missed case. Conclusion Missed diagnosis exists in simple colposcopy or ThinPrep liquid-based cervical cytology for detecting cervical cancer, but that does not exist if combining the two methods for detecting cervical cancer, and the diagnosis of early cervical cancer by combining the two methods is conducive to im-proving the detection rate of the disease, which is worthy of clinical popularization and application.

  2. Self-perceived risk and barriers to cervical cancer screening among patients seeking care at a tertiary care teaching hospital in Tamil Nadu

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    Lokeshwari Jayaraman

    2016-03-01

    Conclusions: self-perception of not being at risk is documented to be associated with low uptake of screening. Concerned health education programs need to bring clearly to the end user the difference between precancerous lesions and invasive cervical cancer and the treatment options available. [Int J Res Med Sci 2016; 4(3.000: 766-769

  3. High grade squamous intraepithelial lesion in inmates from Ohio: cervical screening and biopsy follow-up

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    Rofagha Soraya

    2006-01-01

    Full Text Available Abstract Background Cervical carcinoma remains the second leading cause of cancer death in women worldwide and sexual behavior is regarded as the main contributing factor. We studied cervical cytology screening with surgical biopsy follow-up in women prisoners and compared the findings to those in the general population. Methods We reviewed 1024 conventional cervical smears, 73 cervical biopsies and 2 loop electrosurgical excision procedure (LEEP specimens referred to us from the Correctional Center in Columbus, Ohio during a 12-month period. The results were compared to 40,993 Pap smears from the general population for the same 12-month period. Results High grade squamous intraepithelial lesion (HGSIL was diagnosed in 1.3% of the cervical smears from the inmate population versus 0.6% in the general population (p < 0.01. The unsatisfactory rate was 1.6% compared to 0.3% in the general population (p < 0.01. Among the study population, follow-up tissue diagnosis was obtained in 24.3% of the abnormal cytology results (ASCUS, LGSIL, and HGSIL. Of the HGSIL Pap smears, 61.5% had a subsequent tissue diagnosis. Thirty-nine biopsies (52% of the all inmate biopsies and LEEP showed CIN II/III (cervical intraepithelial neoplasia II/III. Eight of these thirty-nine follow-up biopsies diagnosed as CIN II/III had a previous cervical cytology diagnosis of ASCUS. The average age for HGSIL was 30.5 years (S.D. = 5.7 and for low grade squamous intraepithelial lesion (LGSIL was 27.2 years (S.D. = 6.1. Conclusion A significantly higher prevalence of HGSIL cervical cytology and unsatisfactory smears was encountered in female inmates, with tissue follow-up performed in less than two thirds of the patients with HGSIL. These results are in keeping with data available in the literature suggesting that the inmate population is high-risk and may be subject to less screening and tissue follow-up than the general population. Clinicians should proceed with urgency to improve

  4. Effect of hrHPV infection on anti-apoptotic gene and pro-apoptotic gene expression in cervical cancer tissue

    Institute of Scientific and Technical Information of China (English)

    Min-Er Tang

    2016-01-01

    Objective:To study the effect of hrHPV infection on anti-apoptotic gene and pro-apoptotic gene expression in cervical cancer tissue.Methods: A total of 56 patients with cervical cancer, 94 cases of patients with cervical intraepithelial neoplasia and 48 cases of patients with chronic cervicitis who were treated in our hospital from May 2013 to December 2015 were selected for study and included in malignant group, precancerous lesion group and benign group respectively. hrHPV infection as well as the expression of anti-apoptotic genes and pro-apoptotic genes in cervical tissue were detected.Results:hrHPV infection rate and viral load in cervical tissue of malignant group were significantly higher than those of precancerous lesion group and benign group; P27 and p16 levels in cervical tissue of malignant group were significantly lower than those of precancerous lesion group and benign group, and K-ras, c-myc, Prdx4 and TNFAIP8 levels were significantly higher than those of precancerous lesion group and benign group; the greater the HPV virus load, the lower the p27 and p16 levels and the higher the K-ras, c-myc, Prdx4 and TNFAIP8 levels in cervical tissue.Conclusions:hrHPV infection can result in tumor suppressor genes p27 and p16 expression deletion and increase the expression of proto-oncogene and apoptosis-inhibiting genes, and it is associated with the occurrence and development of cervical cancer.

  5. Cervical Liquid-based Cytology in Cervical Lesions the Pathological Diagnosis of Significance%宫颈液基薄层细胞学在宫颈病变病理诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    郑国文

    2013-01-01

      Objective To investigate the liquid-based cytology (TCT) value in pathological diagnosis of cervical lesions. Methods Retrospective analysis of 2009 January to 2012 June, 5795 patients with TCT cervical cytology, colposcopy biopsy positive cases, compared the two results. Results In 5499 cases of TCT were negative, 296 cases were positive, the positive rate was 5.11%, false negative in 15 cases, no false positive cases. 17 cases of biopsy tissue inflammation, 224 cases of CIN Ⅰ, 20 cases of CINⅡ, 30 cases of CINⅢ, CA 5 cases, LSIL and CIN Ⅰcoincidence rate was 80% (36/45), HSIL and CINⅡ/Ⅲcoincidence rate was 88.9% (24/27). Conclusion TCT examination has a little injury, high positive rate, strong repeatability, for married women can be used as the main method of screening for cervical cancer and precancerous lesions.%  目的探讨液基薄层细胞学(TCT)检查在宫颈病变病理诊断中的意义。方法回顾分析我院2009年1月至2012年6月5795例患者运用 TCT 进行宫颈脱落细胞学检查,阳性病例在阴道镜下活检,二者结果相比较分析。结果 TCT 检查阴性5499例,阳性296例,阳性率5.11%,假阴性15例,无假阳性病例。组织活检炎症17例,CIN Ⅰ224例,CIN Ⅱ20例,CIN Ⅲ30例,CA 5例,LSIL 与 CIN Ⅰ符合率80.0%(36/45),HSIL 与 CIN Ⅱ/Ⅲ符合率88.9%(24/27)。结论 TCT 检查具有损伤小,阳性检出率高,重复性强,对已婚妇女可作为宫颈癌及癌前病变筛查的主要方法。

  6. Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea

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    Carro-Campos Patricia

    2009-09-01

    Full Text Available Abstract Background The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most frequent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. Methods A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP. Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types. Results Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%. HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection. Conclusion The frequency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31 should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.

  7. 多环黏膜切除术治疗早期食管癌及癌前病变的临床价值%Clinical value of multiband mucosectomy for the treatment of early esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    柴同海; 靳西凤; 张晶; 孙庆燕; 李曙晖

    2013-01-01

    目的 探讨多环黏膜切除术(MBM)治疗早期食管癌及癌前病变的临床价值.方法 回顾性分析2010年1月至2011年1月山东济宁医学院附属滕州市中心人民医院收治的43例食管癌以及癌前病变患者的临床资料.43例患者(13例早期癌、30例癌前病变)共51个病灶经术前内镜检查及黏膜活组织检查证实后行MBM治疗.术后1、3、6、12个月门诊行内镜检查并于原病灶处进行活组织检查,以后每6个月定期复查内镜.结果 43例患者共行52次MBM,共切除标本180份;切除病灶大小为10 mm×8mm~25 mm×23 mm;手术时间为(37±5)min.病灶完全切除率为92.2% (47/51).4例未完全切除病灶的患者中,1例早期食管癌患者术后病理检查示鳞状细胞癌侵犯黏膜下层,切缘见癌细胞浸润而追加手术治疗;1例鳞状上皮癌患者术后2周追加放射治疗;其余2例患者追加氩离子凝固术(APC)治疗.52次MBM术中出现4次创面出血,治疗过程中均未出现食管穿孔.1例患者术后10 d发生延迟性出血,行APC止血;1例发生食管轻度狭窄,无需扩张治疗,6个月后逐渐恢复.手术前后病理检查结果准确率为51.0%(26/51).43例患者随访1年,3例复发,复发患者行MBM或APC治疗,无一例患者死亡.结论 MBM治疗早期食管癌及癌前病变简便、安全、有效,有较好的应用前景.%Objective To investigate the clinical value of multiband mucosectomy (MBM) for the treatment of early esophageal cancer and precancerous lesions.Methods The clinical data of 43 patients with early esophageal cancer or precancerous lesions (including 13 cases of early esophageal cancer and 30 cases of precancerous lesion) who were admitted to the Tengzhou Central People's Hospital from January 2010 to January 2011 were retrospectively analyzed.A total of 51 lesions were confirmed by preoperative endoscopy and mucosal biopsy,and then were treated by MBM.Results A total of 52 MBM procedures were

  8. Assessment of the natural course and treatment of premalignant uterine cervical lesions in pregnancy

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    Šljivančanin Dragiša

    2013-01-01

    Full Text Available Introduction. Premalignant changes of the uterine cervix occur with similar frequency during pregnancy and in non­pregnant women. Due to the fact that any surgery on the cervix can jeopardize pregnancy, it is important to define the protocol of procedures for the treatment of these changes during pregnancy. Objective. The aim of the study was to investigate the natural course of premalignant cervical changes during pregnancy and the impact of their treatment on the pregnancy course. Methods. Study involved all patients with colposcopically, cytologically and hystopathologically diagnosed premalignant cervical changes during pregnancy from 2002 to 2008. Patients were divided into two groups according to the applied treatment during pregnancy: surgery or monitoring by regular colposcopic and cytological examinations. The two groups were compared concerning treatment outcome, persistence or regression of changes and pregnancy duration. Results. Study involved 58 patients. Spontaneous remission of lesions occurred after pregnancy in 63.79% of cases. Highgrade squamous intraepithelial lesion (H­SIL demonstrated a higher rate of persistency in comparison with low­grade squamous intraepithelial lesion (L­SIL (χ2=25.115; p<0.05. Only one finding of L­SIL progressed into H­SIL in the monitored group. Patients who underwent conization during pregnancy had a significantly more frequent preterm deliveries (χ2=14.369; p<0.05. Conclusion. The obtained high rate of spontaneous regression of cervical changes after pregnancy as well as the lower incidence of preterm births in patients who were not treated by conization during pregnancy, confirm that patients with premalignant cervical changes should be, if invasion is excluded, under follow­up throughout pregnancy by regular colposcopic and cytological examinations. Therapeutic conization, due to numerous complications, should be performed only when there is a suspected presence of a more severe form of

  9. Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer.

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    Nina Milutin Gašperov

    Full Text Available Change in the host and/or human papillomavirus (HPV DNA methylation profile is probably one of the main factors responsible for the malignant progression of cervical lesions to cancer. To investigate those changes we studied 173 cervical samples with different grades of cervical lesion, from normal to cervical cancer. The methylation status of nine cellular gene promoters, CCNA1, CDH1, C13ORF18, DAPK1, HIC1, RARβ2, hTERT1, hTERT2 and TWIST1, was investigated by Methylation Specific Polymerase Chain Reaction (MSP. The methylation of HPV18 L1-gene was also investigated by MSP, while the methylated cytosines within four regions, L1, 5'LCR, enhancer, and promoter of the HPV16 genome covering 19 CpG sites were evaluated by bisulfite sequencing. Statistically significant methylation biomarkers distinguishing between cervical precursor lesions from normal cervix were primarily C13ORF18 and secondly CCNA1, and those distinguishing cervical cancer from normal or cervical precursor lesions were CCNA1, C13ORF18, hTERT1, hTERT2 and TWIST1. In addition, the methylation analysis of individual CpG sites of the HPV16 genome in different sample groups, notably the 7455 and 7694 sites, proved to be more important than the overall methylation frequency. The majority of HPV18 positive samples contained both methylated and unmethylated L1 gene, and samples with L1-gene methylated forms alone had better prognosis when correlated with the host cell gene promoters' methylation profiles. In conclusion, both cellular and viral methylation biomarkers should be used for monitoring cervical lesion progression to prevent invasive cervical cancer.

  10. Cervical Lesions and Cellular Atypia in a Female Population from Transylvania

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    Lucia C FETICU SIMONCA

    2013-12-01

    Full Text Available Introduction: The purpose of this study was to detect the types of the cervix lesions and to establish the correlations between age, environment of origin, diagnosis and gravity of the lesions. Methods: In the period 2009-2012, all cervical secretions from female subjects presented at Integrated Outpatient Unit, of the Clinical Hospital for Infectious Diseases, Cluj-Napoca, Romania were tested by Babeş-Papanicolaou examination. Babeş-Papanicolaou cytological smear was performed according to the 2001 Bethesda System criteria. Results: From 3153 cervical secretions (2736 female subjects from urban areas and 417 female subjects from rural areas with age 10 - 87 years, 2899 (91.9% smears (372 women from rural area and 2527 women from urban area had normal appearance. Premalignant or malignant lesions (positive cases were detected in 254 (8.1% smears (45 (12.1% cases from rural area, 209 (8.3% cases from urban area. In the urban area, most positive cases were recorded in the age range of 45-54 years, while in the rural areas in the age range of 35-44 years. The multivariate logistic regression analysis showed that environment and age significantly influenced the occurrence of positive cases (OR=1.44 95% CI 1.02-2.02, p=0.04 for rural area, OR=0.71 95% CI 0.60-0.85, p<0.001 for age. The correlation between age and the degree of severity diagnosis (r=-0.09, p=0.14 was not significant. Conclusions: Cervical lesions detected through Babeş-Papanicolaou test in adult women are more common in the rural area.

  11. One-year clinical evaluation of compomer restorations in cervical lesions of different aetiology

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    Premović Milica

    2012-01-01

    Full Text Available Introduction. The aim of this one-year prospective clinical study was to evaluate the treatment results of compomer restorations (Dyract® eXtra/ Dentsply/De Trey, Konstanz, Germany with a single step self-etching dental adhesive (Xeno® III Dentsply/De Trey, Konstanz, Germany used for restoring class V lesions (non-carious and primary carious cervical lesions. Material and Methods. A total number of 62 class V restorations (n = 62 were placed by one dentist in 30 patients on incisors, canines and premolars. The fillings were placed due to different indications: non-carious cervical defects (n = 32 and primary carious lesions (n = 30. The restorations were evaluated by a single-blind design, according to the Modified United States Public Health Service system 6 and 12 months following the placement. The following were evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. The statistical analysis compared the ratings of each criteria between materials using the Pearson chi-square or Fisher’s exact test at a level of significance of 5% (p<0.05. Results. Two restorations of the non-carious lesion group were lost after 6 months, and after 12 months one restoration was lost in the group of primary carious lesions. There were no statistically significant differences between restorations for all evaluated criteria in both groups. Conclusion. The compomer restorations in combination with a single step self-etching dental adhesive showed acceptable clinical performance in Class V lesions after one year of clinical service.

  12. Cervical intraepithelial lesions in females attending Women′s Health Clinics in Alexandria, Egypt

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    Mona Abdel-Hadi

    2015-01-01

    Full Text Available Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to have a representative sample for women living in Alexandria. All women included did not have any cervical disorder related complaints. Conventional Pap smears were obtained and diagnosed using the Bethesda system. Women with abnormal Pap smears were managed according to the 2006 consensus guidelines within the available facilities. Persistent abnormal cytological results were referred for colposcopic biopsy. Histological results were grouped into: Reactive changes, CIN 1, CIN 2/CIN 3 and adenocarcinoma in-situ (AIS. Results: Out of the 6173 smears included in the study 6072 (98.36% were normal and only 101 (1.63% were abnormal. After colposcopic biopsies, 0.08% had CIN 1, 0.03% had CIN 2, 3 and 0.01% had AIS. Conclusion: We concluded that cervical cancer screening programs, although life-saving for a number of women, are not a sufficiently high priority in our community. Money for national health screening programs should preferably be directed more towards recruiting women for breast cancer screening, since breast cancer accounts for about 33% of all female cancers in Egypt ranking number one, while cervical cancer ranks number 13.

  13. Bone marrow mesenchymal stem cell transplantation for gastric precancerous lesions in a rat model%骨髓间充质干细胞移植对大鼠胃癌前病变模型的修复作用

    Institute of Scientific and Technical Information of China (English)

    刘洪锋; 李潞

    2016-01-01

    背景:胃癌前病变是多种因素共同参与的长期发展过程,骨髓间充质干细胞有修复组织损伤的作用。目的:探讨骨髓间充质干细胞移植对大鼠胃癌前病变的影响。  方法:将36只Wistar大鼠随机分为对照组、模型组和移植组。模型组和移植组建立大鼠胃癌前病变模型,移植组大鼠经尾静脉注射1 mL CM-dil标记的骨髓间充质干细胞(3×106个细胞),每周注射1次,一共3次,模型组和对照组同样方法经尾静脉注入等量的生理盐水。末次注射后1周处死大鼠,观察大鼠胃组织病理变化,检测胃黏膜中血管内皮生长因子的表达以及血清中细胞因子含量。  结果与结论:①移植组大鼠胃黏膜损伤较模型组轻,移植组血管内皮生长因子表达明显高于模型组和对照组(P<0.05);②模型组和移植组血清白细胞介素17和γ-干扰素水平高于对照组(P<0.05),移植组血清白细胞介素17和γ-干扰素水平低于模型组(P<0.05);③结果表明,骨髓间充质干细胞通过增加胃黏膜病变部位血管内皮生长因子的表达,降低血清白细胞介素17和γ-干扰素水平,从而提高血管通透性,减轻炎症反应,阻断或者缓解癌前病变的发生。%BACKGROUND:Precancerous lesions are a long-term development process in which many factors are involved. Bone marrow mesenchymal stem cel s can repair tissue injury. OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cel transplantation on gastric precancerous lesions in the rats. METHODS:Thirty-six Wistar rats were randomly divided into control group, model group and transplantation group. Animal models of gastric precancerous lesions were established in the model and transplantation groups. Rats in the transplantation group were given 1 mL of CM-dil-labeled bone marrow mesenchymal stem cel s (3×106 cel s) via the tail vein, once a week, total y three times. Rats in the

  14. Alternative rubber dam isolation technique for the restoration of Class V cervical lesions.

    Science.gov (United States)

    Owens, Barry M

    2006-01-01

    This article describes an expedited, atraumatic technique of restoring cervical abrasion-erosion, abfraction or carious lesions using an alternative placement sequence of the dental rubber dam for adequate field isolation. As shown by this technique, the rubber dam retainer is modified and positioned on the tooth with subsequent placement of the dental dam material over theretainer and tooth. This technique saves time and provides good retraction of the gingival tissue and isolation of the cavity preparation from contamination (saliva, hemorrhage) that can potentially cause post-operative symptoms and possible restoration replacement.

  15. Diagnostic analysis of endoscopic Lugol liquid staining for early esophageal cancer and precancerous lesions%内镜下卢戈氏液染色对早期食管癌及癌前病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李琪毅; 姜仕柱; 李素玉

    2013-01-01

    目的 探讨卢戈氏液染色对早期食管癌及癌前病变的诊断价值.方法 对本院内镜室2011年11月-2013年4月的187例患者的食管表浅病灶进行卢戈氏液染色,并行病理学检查,将病理结果与染色结果进行对比.结果 187例患者中经病理确诊为食管鳞癌41例,食管重度不典型增生9例,食管中度不典型增生33例,食管轻度不典型增生15例,慢性食管炎58例,鳞状上皮增生31例.卢戈氏液染色后食管癌及癌前病变主要表现为卢戈氏液不染和淡染,卢戈氏液淡染和不染诊断食管癌与癌前病变的准确性为62.82%(98/156);食管癌和食管重度不典型增生主要表现为卢戈氏液不染,其准确性高达95.6%(45/48).结论 内镜下卢戈氏液染色对鉴别食管癌、癌前病灶与炎性病灶等具有十分重要的临床价值.%Objective To investigate the clinical and pathological diagnosis of Lugol solution to early esophageal cancer and precancerous lesions.Methods One hundred and eighty-seven patients with suspicious lesions of esophageal endoscopy in our hospital from Nov.2011 to Apr.2013 were chosen as study objects and were dyed with Lugol of the liquid and were given pathological examination.Pathology results and the results of dye were compared.Results Forty-one out of the 187 cases were found with esophageal squalors carcinoma,9 with severe esophageal dysplasia,33 with moderate esophageal dysplasia,15 with esophageal mild dysplasia,58 with chronic esophagi,31 with squalors cell hyperplasia.After Lugol solution dyeing,the signifying of esophageal cancer and precancerous lesions was not pollution or light pollution,and the accuracy was 62.82%.Lugol non-staining liquid esophagus and esophageal cancer diagnosis of severe dysplasia was as high as 93.75% in accuracy.Conclusion Endoscopic Lugol solution staining confined with superficial esophageal biopsy of the lesion have important value in diagnosing superficial esophageal lesion.

  16. Clinicopathological significance and relations of Caspase-3 expression, cell proliferation and apoptosis in gastric cancer and the precancerous lesions%乳腺癌钼靶X线表现特征(118例分析)

    Institute of Scientific and Technical Information of China (English)

    XiaoBin Hu; Jing Zhao; Lin Yang; Yan Xin

    2009-01-01

    Objective: we investigated the relationship between the expression of Caspase-3. cell proiferation and apoptosis in gastric cancer and their precancerous lesions, to explore the tumorigenesis of the stomach mucosa. Methods: Caspase-3 expression in 13 normal gastric mucosa, 6 chronic atrophic gastritis (CAG), 31 intestinal metaplasia (IM), 114 dysplasia (DYS) and 20 gastric carcinomas were investigated immunohistochemically. Cell proliferation was evaluated with anti-Ki-67immunostaining and apoptosis was evaluated using DNA fragmentation in situ by TdT-mediated dUTP biotin nick end label-ing (TUNEL) method. Results: Caspase-3 mild-moderately positive expression was observed in most of normal superficial epithelia, its positively polar distribution in normal mucosa, CAG, IM, DYS and gastric carcinomas changed as seen in TU-NEL, and so did the positive rate. Caspase-3 protein expression showed significantly positive correlation with the number of apoptotic cells labeled with TUNEL (correlation coefficient r=0.94; P 0.05). Conclusion: Caspase-3 protein expression was up-regulated from CAG to IM and mild-moderate atypical dysplasia, but down-regulated in severe dysplasia and gastric carcinoma, indicating that inactivity or reduced expression of Caspase-3 is closely correlated with carcinogenesis of the stomach mucosa.

  17. 免疫营养剂对胃癌癌前病变的调理作用%Effects of lmmunonutrition in Precancerous Lesions of Gastric Cancer in Rats

    Institute of Scientific and Technical Information of China (English)

    向连斌; 陈振勇

    2011-01-01

    Objective: To investigate the effect of immunonutrition formula precancerous lesions of gastric cancer in rats. Methods: The rats model with precancerous lesions of gastric cancer was established by MNNG, and 90 rats were randomized into experimental group, model group and control group. Animals in model group were fed with common diets, the experimental group were fed with an enteral diet supplemented with immunonutrition formula (120 ml/[kg · d]). The changes of the gastric mucosa were observed under microscope after 4, 8, and 12 weeks respectively. The levels of blood IgG, IgA, IgM, CD3+ ,CD4+ , and CD8+ , as well as AgNOR positive cells counting were examined. Results: In model group, gastric mucosa got thin and atrophied,and intestinal metaplasia were seen, while in experimental group, the mucosa showed no changes. The immune function of rats with precancerous lesions of gastric cancer was inhibited, IgA and IgM levels were significantly reduced, and the ratio of CD4+/CD8+ T-cell decreased (all P< 0.05). After feeding with immunonutritions for 12 weeks, the level of IgA and IgM resumed normal, the radio of CD4+/CD8+ raised, and the AgNOR positive cells counting decreased (all P< 0.01). Conclusion: Immunonutritions could effectively reverse the precancerous lesions in gastric carcinoma and its mechanisms of reversion probably relate to the enhance immunological function and nutritional status.%目的:研究免疫营养制剂对大鼠胃癌癌前病变阶段的影响.方法:制造大鼠胃癌癌前病变的模型,90只大鼠随机分为对照组、模型组和实验组,模型组采用普通的饲料喂养,实验组在普通的饲料喂养的基础上添加免疫加强营养制剂瑞能[(120 ml/(kg·d)].分别在造模成功后4,8,12周显微镜下观察鼠胃黏膜的变化,AgNOR染色观察细胞的增殖和分化,并抽血检测IgG、IgA、IgM及T淋巴细胞亚群CD3+、CD4+和CD8+T细胞.结果:处于癌前病变阶段的大鼠胃黏膜表面部分上

  18. Correlation of abnormal DNMT1 and MeCP2 expression with cell biological characteristics in cervical lesion tissue

    Institute of Scientific and Technical Information of China (English)

    Wei Lin; Sha Ma

    2016-01-01

    Objective:To study the correlation of abnormal DNMT1 and MeCP2 expression with cell biological characteristics in cervical lesion tissue.Methods:Cervical cancer tissue and para-carcinoma tissue were collected from cervical cancer patients who received surgery in our hospital from May 2012 to October 2015, and HPV types as well as the expression levels of DNMTs, MeCP2, PBK, TOPK, Snail, Slug, SALL4 and Cat L were determined.Results:Protein levels of DNMT1, DNMT2, DNMT3a, DNMT3b, DNMT3l and MeCP2 in cervical cancer tissue were significantly higher than those in para-carcinoma tissue, and the rising trend of DNMT1 expression level was the most significant; protein levels of DNMT1, DNMT2, DNMT3a, DNMT3b, DNMT3l and MeCP2 in cervical cancer tissue with high-risk HPV infection were significantly higher than those in cervical cancer tissue with normal HPV infection; in cervical cancer tissue with high expression of DNMT1 and MeCP2, PBK, TOPK, Snail, Slug, SALL4 and Cat L levels were significantly higher than those in cervical cancer tissue with low expression of DNMT1 and MeCP2.Conclusions:Abnormally high expression of DNMT1 and MeCP2 in cervical cancer tissue may up-regulate the expression of a variety of malignant biological molecules by increasing methylation level.

  19. Clinical correlative study on early detection of oral cancer and precancerous lesions by modified oral brush biopsy and cytology followed by histopathology

    Directory of Open Access Journals (Sweden)

    Swati Gupta

    2014-01-01

    Conclusion: The results demonstrate that modified oral brush biopsy has higher efficacy than routine cytology and can be used as a potentially practical oral cancer screening tool in resource challenged settings. However, clinical judgment is of prime importance. Immediate biopsy is mandatory in highly suspicious lesions proposed under the diagnostic criteria of "clinically diagnosed carcinoma in situ".

  20. MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Cervical cancer is the main cause of death inwomen.The influence of HPV plays an i mportantrole incervial cancer.It has been provedthat humanpapillomavirus(HPV)infectionis ani mportant fac-tor in cervical carcinogenesis.Multiple HPVinfec-tion was associated less frequently with cervical car-cinoma and with precancerous lesions compared withnor mal cytology[1].The activation of oncogene,in-activition of tumor suppressor gene and instabilityof genome are also majority reason.We establisheda cell line of human...

  1. Factors associated to cervical lesions or presence of human papiloma virus in two populations of students from Lima

    OpenAIRE

    Valderrama C, María; Unidad de Epidemiología ETS y VIH, Facultad de Salud Pública, Universidad Peruana Cayetano Heredia. Lima, Perú. Obstetriz.; Campos, Francisco E.; Hospital Nacional Docente Madre Niño San Bartolomé. Lima, Perú. médico infectólogo pediatra.; Cárcamo, César P; Unidad de Epidemiología ETS y VIH, Facultad de Salud Pública, Universidad Peruana Cayetano Heredia. Lima, Perú. Departamento de Microbiología, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia. Médico Epidemiólogo. Médico Infectólogo.; García, Patricia J; Unidad de Epidemiología ETS y VIH, Facultad de Salud Pública, Universidad Peruana Cayetano Heredia. Lima, Perú. Instituto Nacional de Salud. Lima, Perú. Médico Epidemiólogo. Médico Infectólogo.

    2007-01-01

    Objectives: To determine the prevalence and factors associated with cervical lesions or presence of human papilloma virus (HPV) in women students with higher education from 18 to 26 years. Materials and methods: A cross-sectional study in students from two universities and a technical institute in Lima were carried out from August through December 2001. We surveyed women and collected cervical samples for PAP smear and HPV DNA detection for the 6, 11, 16 and 18 strains using polymerase ch...

  2. Revised terminology for cervical histopathology and its implications for management of high-grade squamous intraepithelial lesions of the cervix.

    Science.gov (United States)

    Waxman, Alan G; Chelmow, David; Darragh, Teresa M; Lawson, Herschel; Moscicki, Anna-Barbara

    2012-12-01

    In March 2012, the College of American Pathologists and American Society for Colposcopy and Cervical Pathology, in collaboration with 35 stakeholder organizations, convened a consensus conference called the Lower Anogenital Squamous Terminology (LAST) Project. The recommendations of this project include using a uniform, two-tiered terminology to describe the histology of human papillomavirus-associated squamous disease across all anogenital tract tissues: vulva, vagina, cervix, penis, perianus, and anus. The recommended terminology is "low-grade" or "high-grade squamous intraepithelial lesion (SIL)." This terminology is familiar to clinicians, because it parallels the terminology of the Bethesda System cytologic reports. Biopsy results using SIL terminology may be further qualified using "intraepithelial neoplasia" (IN) terminology in parentheses. Laboratory p16 tissue immunostaining is recommended to better classify histopathology lesions that morphologically would earlier have been diagnosed as IN 2. p16 is also recommended for differentiating between high-grade squamous intraepithelial lesions and benign mimics. The LAST Project recommendations potentially affect the application of current guidelines for managing cervical squamous intraepithelial lesions. The authors offer interim guidance for managing cervical lesions diagnosed using this new terminology with special attention paid to managing young women with cervical high-grade squamous intraepithelial lesions on biopsy. Clinicians should be aware of the LAST Project recommendations, which include important changes from prior terminology.

  3. 早期食管癌及癌前病变多环黏膜切除60例报告%Multi-band Mucosectomy for Early-stage Esophageal Carcinoma and Precancerous Lesions:Report of 60 Cases

    Institute of Scientific and Technical Information of China (English)

    黄涛; 张红; 杨国栋; 冯迎春; 凌颖; 蒋玲; 冯志松

    2015-01-01

    目的:探讨多环黏膜切除术( multi-band mucosectomy , MBM )治疗早期食管癌及癌前病变的疗效和安全性。方法我院2011年12月~2013年10月对60例胃镜及病理确定的早期食管癌及癌前病变,胃镜下1.2%卢戈液染色,确定病灶并标记切除范围,安装多环黏膜切除器,自口侧将病灶上缘及标记点上皮层吸入,释放橡胶环完成套扎,圈套器电凝切除,收集组织标本;重复吸引、套扎、切除至病灶完全切除。结果60例共84处病灶,1例术中出血转胸外科手术,其余59例83处(98.3%)病灶均一次性成功切除。使用皮圈1~12发,共用皮圈289发,平均4.8发/例。手术时间10~60 min,平均23.5 min;切除长度1.0~10 cm,宽度不超过3/4食管周径;3例(5.0%)术中明显出血,2例术中止血,1例转外科手术,术后无一例出血。术中穿孔1例(1.7%),置入全覆膜金属支架保守治疗成功。术后病理:全部标本基底无癌残留,原位癌9例,高级别瘤变29例,低级别瘤变12例,角化不全6例,颗粒细胞瘤1例,息肉/炎性增生3例。59例随访1~23个月,2例(3.4%)食管狭窄,经探条扩张吞咽困难缓解,无一例局部复发及发现淋巴结转移。结论 MBM治疗早期食管癌及癌前病变近期疗效确切,安全可靠;技术要求相对较低,值得广泛推广。%Objective To study the efficacy and safety of multi-band mucosectomy (MBM) for the treatment of early-stage esophageal carcinoma and precancerous lesions . Methods A total of 60 cases of early-stage esophageal carcinoma and precancerous lesions ( diagnosed pathologically ) were admitted from December 2011 to October 2013 in this hospital .The scope of lesion was determined by using 1.2% Lugo staining and the target area was delineated with coagulation marking .After the installation of polycyclic mucosal resection device , the

  4. Impact of timing of antiretroviral therapy initiation on survival of cervical squamous intraepithelial lesions: a cohort analysis from South Africa.

    Science.gov (United States)

    Zeier, M D; Nachega, J B; Van Der Merwe, F H; Eshun-Wilson, I; Van Schalkwyk, M; La Grange, M; Mason, D; Louw, M; Botha, M H

    2012-12-01

    To determine factors that influence excision treatment outcome and recurrence of cervical squamous intraepithelial lesions (SIL) in women living with HIV infection, we analysed 1848 women who underwent excision treatment of cervical SIL at Tygerberg Hospital, Cape Town, South Africa. We compared treatment failure defined as presence of cervical intraepithelial neoplasia (CIN) I (presence of CIN I or higher at first follow-up after excision treatment) and post-excision recurrence of lesions (at one year or later) between women of HIV-positive, -negative or unknown status and examined factors associated with excision treatment outcome and recurrence. HIV-infected women experienced higher treatment failure than uninfected women (53.8% versus 26.9%, P treatment failure, more HIV-infected women had low-grade squamous intraepithelial lesion (LSIL) compared with uninfected women (64.9% versus 37.3%, P Treatment failure did not differ with the type of excision used in HIV-infected women. HIV-infected women were more likely to experience recurrence of lesions after excision treatment than uninfected women (hazard ratio 1.95, 95% confidence interval [CI] 1.59-2.39; P cervical SIL initiated on ART earlier may be expected to have better long-term excision treatment outcome. Close follow-up should be maintained after cervical excision treatment, especially in a setting of high HIV prevalence.

  5. Viral DNA load of high-risk human papilloma virus is closely associated with the grade of cervical lesions

    OpenAIRE

    Shen, Guqun; Cheng, Jingxin; Wang, Yan; Zhou, Ping; Zhang, Guoqing

    2014-01-01

    This study is to explore the correlation between the viral load of high-risk human papilloma virus (HPV) and the degree of cervical lesions, as well as the follow-up monitoring role of high-risk HPV measurements in the treatment of patients with cervical lesions. Hybrid capture-2 method was used to measure the amount of high-risk HPV load of 361 patients who were enrolled from January 2009 to December 2010 at the Affiliated Tumor Hospital of Xinjiang Medical University, including 76 cases of ...

  6. 不同检测方法在宫颈病变和早期宫颈癌筛查中的价值%The Screening Value of Different Detection Methods for Cervical Lesions and Early Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张雯; 安红梅; 马丽莎; 邓继红

    2011-01-01

    of invasive carcinoma). The highest sensitivity and Youden index ofHC-E test to diagnose the cervical le-sbnsmore servere than the high grade squanous intraepithelial lesbr( HSIL) were92.31% and0.81 respectively TCT, VIA, cot poscopy and Pap snearhad amoderate degree of sensitivity to diagnose cervical lesions more servere than the HSIL HPV-DNA+ TCT diagnosis was with the highest sensitivity, specificity and Youden index to diagnose cervical lesions more servere than the HSIL Con-clisim : TCT combined with HPV test is the optmal choice for the screening of early cervical lesions and cervical cancer the second recommended program is Pap snear+ HPV test; The general program consists ofHPV test and colposcopy, and the fundamental program vs comprised ofPap smear+ VIA. Complementarymulti-technobgy screening can find a large numberof precancerous lesions and cervical cancer Medical staff should select the appropriate screening program according to the patients" actual situation

  7. Comparative evaluation of genetic assays to identify oral pre-cancerous fields

    NARCIS (Netherlands)

    Bremmer, J.F.; Braakhuis, B.J.; Brink, A.; Broeckaert, M.A.; Beliën, J.A.M.; Meijer, G.A.; Kuik, D.J.; Leemans, C.R.; Bloemena, E.; van der Waal, I.; Brakenhoff, R.H.

    2008-01-01

    Background: Oral squamous cell carcinomas often develop in a pre-cancerous field, defined as mucosal epithelium with cancer-related genetic alterations, and which may appear as a clinically visible lesion. The test characteristics of three genetic assays that were developed to detect pre-cancerous f

  8. Clinical Value Analysis of Endoscopic Indigo Carmine-Methylene Blue Double Staining in Diagnosis of Early Gastric Cancer and Precancerous Lesions%内镜下靛胭脂-美兰双重染色法诊断早期胃癌及癌前病变的临床价值分析

    Institute of Scientific and Technical Information of China (English)

    靳斌; 张永红; 赵梦云; 朱玉侠; 张雪丽; 李蓉; 路伟; 杨芸; 孔祥云; 赵明星; 韦斌

    2015-01-01

    Objective: To investigate clinical value of the endoscopic indigo carmine-methylene blue double staining in diagnosis of early gastric cancer and precancerous lesions.Methods:The patients to seek treatment with routine endoscopy, those of 112 suspicious lesions were randomly divided into two groups, the observation group were taken biopsy after endoscopic indigo carmine-methylene blue staining.The control group were routine biopsy.Results:16 cases of early gastric cancer and 27 cases of precancerous lesions were detected in the observation group (57 cases).9 cases of early gastric cancer and 19 cases of precancerous lesions were detected in the control group (57 cases).The early gastric cancer and precancerous lesion positive rate of the observation group and the control group was 43/57 (75.4%), and 28/55 (50.9%), respectively.The difference was statistically significant (P<0.01).There are obvious staining demarcation zone 28/57 (49.1%) of gastric cancer and precancerous lesion detection rate was significantly higher than those without clear demarcation zone staining 15/57 (26.3%) (P<0.05).Conclusion:Endoscopic indigo carmine methylene blue double staining can significantly improve the detection rate of early gastric cancer and precancerous lesion,that has the certain instruction significance or the early diagnosis of gastric cancer and precancerous lesions.%目的:探讨内镜下靛胭脂-美兰双重染色法对诊断早期胃癌及癌前病变的临床应用价值.方法:来就诊患者行常规内镜检查,将有可疑病灶患者112例随机分为两组,观察组行内镜下靛胭脂-美蓝染色后活检,对照组采用后常规活检.结果:观察组(57例)分别检出早期胃癌16例、癌前病变27例.对照组(55例)分别检出早期胃癌9例、癌前病变19例,观察组与对照组早期胃癌和癌前病变检出率分别为43/57(75.4%)和28/55(50.9%),且差异有统计学意义(P<0.01).有明显染色分界区28/57(49.1%)的胃癌和癌前病变

  9. Multi-band mucosectomy for early esophageal cancer and precancerous lesions%多环黏膜切除术对早期食管癌及癌前病变的治疗价值

    Institute of Scientific and Technical Information of China (English)

    刘晓岗; 陈子洋; 李易; 阳运超; 张仁翼; 李良平

    2012-01-01

    Objective To evaluate the safety and efficacy of multi-band mucosectomy (MBM) for early esophageal cancer and precancerous lesions.Methods Data of 28 patients with early esophageal cancer or precancerous lesions undergoing MBM were reviewed in regarding of procedure complications and follow-up results.Results A total of 32 lesions were resected successfully by MBM in one session,with mean procedure time of 28.3 minutes.The mean diameter of specimens was 12mm.No residual neoplasm was found at the base of any resected specimens.The post-MBM pathological findings consisted of 2 cases of intramucosal cancer,1 case of submucosal cancer,and 25 cases of moderate-severe dysplasia.No perforation,delayed hemorrhage or subcutaneous emphysema occurred.Intraoperative bleeding occurred in 23 cases,including 3 cases of pulsatile bleeding,which were controlled with metal clip,and 20 cases of minor bleeding which were managed with APC or halted automatically at the end of procedure.Chest pain after the procedure occurred in 5 cases and were relieved soon.The patient with submucosal cancer underwent subsequent surgical resection,with no residual cancer in surgical specimen or lymph node metastasis.Twenty seven other cases were followed up endoscopically for 2-12 months.Esophageal stricture occurred in 2 cases,and were successfully relieved by dilatation with stent or bougienage.No recurrent lesion or metastasis were revealed.Conclusion MBM is a relatively safe and effective endoscopic technique for treatment of early esophageal intramucosal cancer and precancerous lesions,but further studies are needed to evaluate the long-term results.%目的 评价多环黏膜切除术(MBM)治疗早期食管癌及其癌前病变安全性和疗效.方法 对28例行MBM治疗早期食管癌及其癌前病变患者的病例资料进行回顾性分析,总结并发症发生情况以及治疗结果和随访情况.结果 28例共32处病变均经一次操作切除,操作时间18~60 min,平均28.3 min

  10. High-grade cervical lesions among women attending a reference clinic in Brazil: associated factors and comparison among screening methods.

    Directory of Open Access Journals (Sweden)

    Neide T Boldrini

    Full Text Available Although screening for cervical cancer is recommended for women in most countries, the incidence of cervical cancer is greater in developing countries. Our goal was to determine the prevalence and factors associated with high-grade lesions/cervical cancer among women attending a reference clinic in Brazil and evaluate the correlation of histology with cytology, colposcopy and the high-risk HPV (HR-HPV tests.A cross-sectional study of women attending a colposcopy clinic was carried out. The patients were interviewed to collect demographic, epidemiological and clinical data. Specimens were collected for cervical cytology, Chlamydia trachomatis and HPV testing using the Hybrid Capture (HC and PCR tests. Colposcopy was performed for all patients and biopsy for histology when cell abnormalities or cervical intraepithelial neoplasia (CIN were present.A total of 291 women participated in the study. The median age was 38 years (DIQ: 30-48 years. The prevalence of histologically confirmed high-grade lesions/cervical cancer was 18.2% (95%, CI: 13.8%-22.6%, with 48 (16.5% cases of CIN-2/CIN-3 and 5 (1.7% cases of invasive carcinoma. In the final logistic regression model, for ages between 30 and 49 years old [OR = 4.4 (95%: 1.01-19.04, history of smoking [OR = 2.4 (95%, CI: 1.14-5.18], practice of anal intercourse [OR = 2.4 (95%, CI: 1.10-5.03] and having positive HC test for HR-HPV [OR = 11.23 (95%, CI: 4 0.79-26, 36] remained independently associated with high-grade lesions/cervical cancer. A total of 64.7% of the cases CIN-3\\Ca in situ were related to HPV-16. Non-oncogenic HPV were only found in CIN-1 biopsy results. Compared to histology, the sensitivity of cytology was 31.8%, the specificity 95.5%; the sensitivity of colposcopy for high-grade lesions/cervical cancer was 51.0%, specificity was 91.4% and the concordance with HPV testing was high.The results confirm an association of HR-HPV with precursor lesions for cervical cancer

  11. Chemopreventive effect of Prunella vulgaris L. on DMBA-induced oral precancerous lesions%夏枯草对实验性口腔癌化学预防作用的研究

    Institute of Scientific and Technical Information of China (English)

    李芳; 孙正

    2009-01-01

    Objective To study the preventive effect of Prunella vulgaris L. on 7, 12-dimethylbens (a) anthracene (DMBA)-induced oral precancerous lesions in golden Syrian hamsters. Methods The hamsters were divided into three groups. While the negative control group was not treated, the other Syrian hamsters were applied with 0.5% DMBA solution topically to the left cheek pouch three times per week for three consecutive weeks. After the last treatment of DMBA, the hamsters were divided into two groups at random. The positive group was not treated, another group received Prunella vulgaris L. three times within the following week. The animals were disposed of 4 weeks after the start of the ex-periment. They were injected with 5-bromo-2'-deoxyuridine (BrdU) 50rag/kg before they were killed. The samples were collected for histopathology and BrdU immunohistochemisty. Results Prunella vulgaris L. significantly decreased the number of inflammatory cells from 158.65±26.51 / mm2 to 52.25±18.53 /mm2 (P 0.05). Conclusion The results suggest that Prunella vulgaris L. has some effect on the prevention of DMBA-induced oral precancerous lesions, but the effect is not significant on the suppression of cell proliferation and dysplasia.%目的 探讨夏枯草对二甲基苯并蒽(DMBA)诱发的金黄地鼠口腔癌的化学预防作用.方法 2006年3月在首都医科大学口腔医学院研究所将30只金黄地鼠分3组:阴性对照组(6只)不涂药;阳性对照组(12只)涂0.5%DMBA于左侧颊囊,每周3次,共涂3周;夏枯草组(12只)前3周处理同阳性对照组,3周后换涂1%夏枯草,每周3次,涂1周.第4周末实验结束处死所有地鼠,处死前2 h腹腔注射50 mg/kg的5-溴脱氧尿嘧啶(5一bromo-2'-deoxyuridine,BrdU).取左侧颊囊行组织病理学观察和BrdU免疫组化染色.结果 与阳性对照组相比,夏枯草组颊囊单纯增生和异常增生的病灶数目均有所降低,其单纯增生病灶数目与阳性对照组比

  12. Up regulation in gene expression of chromatin remodelling factors in cervical intraepithelial neoplasia

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    Van Niekerk Dirk

    2008-02-01

    Full Text Available Abstract Background The highest rates of cervical cancer are found in developing countries. Frontline monitoring has reduced these rates in developed countries and present day screening programs primarily identify precancerous lesions termed cervical intraepithelial neoplasias (CIN. CIN lesions described as mild dysplasia (CIN I are likely to spontaneously regress while CIN III lesions (severe dysplasia are likely to progress if untreated. Thoughtful consideration of gene expression changes paralleling the progressive pre invasive neoplastic development will yield insight into the key casual events involved in cervical cancer development. Results In this study, we have identified gene expression changes across 16 cervical cases (CIN I, CIN II, CIN III and normal cervical epithelium using the unbiased long serial analysis of gene expression (L-SAGE method. The 16 L-SAGE libraries were sequenced to the level of 2,481,387 tags, creating the largest SAGE data collection for cervical tissue worldwide. We have identified 222 genes differentially expressed between normal cervical tissue and CIN III. Many of these genes influence biological functions characteristic of cancer, such as cell death, cell growth/proliferation and cellular movement. Evaluation of these genes through network interactions identified multiple candidates that influence regulation of cellular transcription through chromatin remodelling (SMARCC1, NCOR1, MRFAP1 and MORF4L2. Further, these expression events are focused at the critical junction in disease development of moderate dysplasia (CIN II indicating a role for chromatin remodelling as part of cervical cancer development. Conclusion We have created a valuable publically available resource for the study of gene expression in precancerous cervical lesions. Our results indicate deregulation of the chromatin remodelling complex components and its influencing factors occur in the development of CIN lesions. The increase in SWI

  13. [Human papillomavirus detection in cervical cancer prevention].

    Science.gov (United States)

    Picconi, María Alejandra

    2013-01-01

    Cervical cancer (CC), which is strongly associated to high-risk human papillomavirus (hr-HPV) infection, continues being a significant health problem in Latin America. The use of conventional cytology to detect precancerous cervical lesions has had no major impact on reducing CC incidence and mortality rates, which are still high in the region. New screening tools to detect precancerous lesions became available, which provide great opportunities for CC prevention, as do highly efficacious HPV vaccines able to prevent nearly all lesions associated with HPV-16 and -18 when applied before viral exposure. Currently, hr-HPV testing represents an invaluable component of clinical guidelines for screening, management and treatment of CC and their precursor lesions. Many testing strategies have been developed that can detect a broad spectrum of hr-HPV types in a single assay; however, only a small subset of them has documented clinical performance for any of the standard HPV testing indications. HPV tests that have not been validated and lack proof of reliability, reproducibility and accuracy should not be used in clinical management. Once incorporated into the lab, it is essential to submit the whole procedure of HPV testing to continuous and rigorous quality assurance to avoid sub-optimal, potentially harmful practices. Recent progress and current status of these methods are discussed in this article.

  14. 喉癌前病变DNA含量测定及细胞周期分析的意义%The clinical significance of DNA content and cell-cycle analysis in laryngeal precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    周剑勇; 梁素霞

    2001-01-01

    Objective To investigate the value of the DNA content and cell-cycle analysis in diagnosing laryngeal precancerous lesion and laryngeal carcinoma. Methods 62 cases including 4 laryngeal normal epithlium, 5 laryngeal keratosis, 8 atypical hyperplaisa cell, 9 papilloma and 36 laryngeal carcinoma were examined with flow cytometry (FCM). Results In continuous process of normal cell to cancerous cell, the DNA index, cell-cycle S-fractions and proliferative index gradually increased, with the appearance of aneuploid cells. Compared with other groups, laryngeal cerainoma was characterized by a high percentage of aneuploid cells (P<0.005). Conclusion The DNA quantitive analysis with FCM is helpful for the early dignosis of laryngeal carcinoma.%目的 探讨DNA含量和细胞周期分析在喉癌前病变诊断中的价值。方法 用流式细胞仪测定正常喉上皮、角化病、不典型增生、乳头状瘤及喉鳞癌共62例患者的DNA含量及细胞周期。结果 从正常喉细胞到癌细胞的变化过程中,DNA含量、S期细胞比例及增殖活性逐渐增加(P<0.01),且出现非整倍体细胞。喉癌细胞非整倍体率达91.7%,超出了其他各组(P<0.005)。结论 流式细胞DNA定量分析有助于喉癌的早期诊断。

  15. Human papilloma virus genotypes in women from Nayarit, Mexico, with squamous intraepithelial lesions and cervical cancer

    Science.gov (United States)

    Ortega-Cervantes, Laura; Aguilar-Lemarroy, Adriana; Rojas-García, Aurora Elizabeth; Barrón-Vivanco, Briscia Socorro; Vallejo-Ruiz, Verónica; León, David Cantú-De; Hernández, Yael Yvette Bernal; Jáuregui-Martínez, Armando; Medina-Díaz, Irma Martha

    2016-01-01

    Objective In Mexico cervical cancer (CC) is the most common cause of death from neoplasia in women. Study aimed to analyze the current distribution of Human papillomavirus (HPV) types in women from Nayarit, Mexico, with Squamous intraepithelial lesions (SIL) and Cervical cancer (CC). Methodology Between January 2011 and July 2013, cervical samples were collected from female residents of the Mexican state of Nayarit and were analyzed by means of a LINEAR ARRAY® HPV genotyping test. Data analyses were performed using Stata ver. 8.0 statistical software. Results Of the samples analyzed, 91.2%, HPV DNA was detected. Of these positive samples, 82% were High-risk (HR) viral types. The most prevalent HPV genotypes identified were 16, 58, 31, 18, and 70. Forty two percent of participants had a single infection, while 23 and 26% of participants were infected with two or more HPV genotypes, respectively. HPV 16 was the most prevalent genotype identified and was frequently present as a co-infection with HPV types 18, 51, 52, 59, 66, or 70. Conclusion Women <20 years of age were most often infected with HPV, and the HPV Quadrivalent vaccine (types 16, 18, 6, and 11), currently available in Mexico, no confers protection against a subset of the HPV genotypes identified in the present study (58, 31, 70, and 35). Thus, it is important evaluate the geographical distribution of specific HPV genotypes in all health of center across Mexico in order to implement a successful vaccination program and to diagnose CC in its early stages. PMID:27610056

  16. The long-term follow-up study in aged people with gastric precancerous lesions%老年人胃癌癌前变化的随访研究

    Institute of Scientific and Technical Information of China (English)

    黄海; 史冬梅; 于晓峰; 赵尚敏; 陈洁

    2012-01-01

    目的 研究老年人胃癌癌前变化的胃癌发病率,确定老年人发生胃癌的危险因素. 方法 完成随访的287例对象入选时平均年龄(79.44±11.69)岁,其中男204例,女83例.在1980年1月至2009年6月期间胃镜病理诊断为胃癌癌前变化,每隔1~3年随访胃镜及活检.对发生胃癌的危险因素进行logistic回归分析,计算OR值及其95%可信区间. 结果 入选对象胃镜病理诊断萎缩性胃炎177例,肠化生75例(其中Ⅰ型36例,Ⅱ型20例,Ⅲ型19例),低级别上皮内瘤变35例.随访共发现胃癌21例,胃癌发生率为7.32%,年平均发生率0.75%.其中继发于萎缩性胃炎4例,胃癌发生率为2.26%,年平均发生率0.23%;Ⅰ型肠化生1例,胃癌发生率为2.78%,年平均发生率0.29%;Ⅱ型肠化生1例,胃癌发生率为5.00%,年平均发生率0.51%;Ⅲ型肠化生6例,胃癌发生率为31.58%,年平均发生率3.25%;低级别上皮内瘤变9例,胃癌发生率为25.71%,年平均发生率2.65%.有吸烟史、低级别上皮内瘤变和Ⅲ型肠化生分别是胃癌发生的危险因素. 结论 老年人胃癌癌前变化的胃癌年平均发生率为0.75%,吸烟、低级别上皮内瘤变和Ⅲ型肠化生是老年人发生胃癌的危险因素.应加强对老年人胃癌癌前变化的胃镜监测.%Objective To determine the incidence and the risk factors of gastric cancer (GC) in aged patients with gastric precancerous lesions. Methods A prospective and retrospective follow-up study was carried out in 287 patients aged 79. 44 ± 11. 69 years (204 men and 83 women) who underwent gastric biopsy with diagnoses of atrophic gastritis (AG) , intestinal metaplasia (IM) and low grade dysplasia (LGD) between January, 1980 and June, 2009. A 1-3 years repeated endoscopy was performed. Multiple logistic regression was used to assess the influence of risk factors of GC, the strength of the influence being expressed as an OR with a 95% CI. Results During a

  17. Study of oral precancerous lesion and oral cancer by using micro PET/CT%Micro PET/CT在大鼠口腔黏膜癌前病变及口腔癌中的应用

    Institute of Scientific and Technical Information of China (English)

    陈梁; 葛姝云; 李晗卿; 周海文

    2012-01-01

    PURPOSE: To study oral precancerous lesion and oral cancer by using micro PET/CT. METHODS: Thirty-nine SD rats were divided into experimental group and control group. 33 of them were raised with a 4-nitroquinoline-l-oxide (4NQO) solution with the concentration of 0.002% during the first 13 weeks, and then changed to normal water. The other 6 rats drank normal water all the time. During 25th to 30th week of the experiment, 2-Deoxy-2-[F-18]fluoro-D-glucose (FDG)-PET/CT was performed for these rats. One day after imaging, pathological examination was performed. SUVmax and T/NT were investigated according to pathological results. SAS6.0 software package was used for statistical analysis. RESULTS: There was no significant difference in SUVmax among the normal group, precancerous group and cancerous group (P>0.05). There was significant difference in T/NT (muscle, brain) between the normal group and the cancerous group (P 0.05); and no significant difference between the precancerous group and the cancerous group (P>0.05). The T/NT (muscle, brain) ratios increased along with the increase of the pathologic grade of the lesions. There was no significant difference in T/NT (thyroid) among the three groups and no correlation between the T/NT (thyroid) ratios and the pathologic grade. CONCLUSIONS: Micro PET/CT, as a non-invasive technology, may contribute to the dynamic studies of the process of carcinogenesis. T/NT (muscle, brain) ratios could show the degree of lesions of rat's tongue during carcinogenesis.%目的:研究Micro PET/CT在大鼠口腔黏膜癌前病变及口腔癌中的应用价值.方法:采用4NQO饮水法诱导大鼠舌癌模型,将39只大鼠随机分为实验组及空白对照组.实验25~30周,进行Micro PET/CT扫描.扫描后行组织病理学检查.应用SAS6.0软件包,对正常组、癌前病变组及癌变组大鼠的SUVmax、T/NT(肌肉)、T/NT(脑组织)及T/NT(甲状腺)分别进行方差分析,并对T/NT值和

  18. Motor Deficit After Cervical Surgery, Parsonage-Turner Syndrome or Root Lesion

    Directory of Open Access Journals (Sweden)

    Ayse Sarifakioglu

    2013-06-01

    Full Text Available A 60 years old male patient was admitted to our outpatient clinic with the complaint of severe pain in right arm after cervical stenosis surgery which had been decreased with the formation of progressive motor weakness. Although clinical and physical evaluation was compatable with Parsonage-Turner syndrome (PTS, C5 root involvement has been detected. The patient was given exercise therapy with TENS for analgesia. The patient%u2019s motor weakness progressed and he had difficulties with daily living activities. With the review of literature, we are presenting a case, whose diagnosis was difficult in terms of differentiating both PTS and root lesion which could only be diagnosed by electroneuromyography.

  19. CT-diagnosis for mass lesions in the parotid gland and cervical region

    Energy Technology Data Exchange (ETDEWEB)

    Matsuyama, F.; Taniguchi, S.; Horii, M.; Suzuki, S.; Shiba, Y. (Kobe Central Municipal Hospital (Japan))

    1982-02-01

    Thirty patients suffering from mass lesion in the parotid gland or cervical region were examined by computed tomography (CT). The photographed images were compared with the findings and pathological diagnoses obtained by surgical procedures. The conclusions were as follows: 1) Plain CT demonstrated the location of the parotid tumor. Contrast enhancement seemed to delineate the margin of the tumor more clearly. 2) By contrast enhancement, the branchiogenic cyst was differentiated from the parotid tumor, as a low density mass with an enhanced cyst wall. 3) Parotid tumors enhanced by contrast material did not always appear as solid tumors. 4) In some cases, CT numbers indicated the contents of the tumor. 5) The extension of the tumor to the parapharyngeal space was clearly depicted on CT. 6) It seemed to be difficult to evaluate the relationship of the parotid tumor to the facial nerve on plain CT.

  20. Prevalence and risk factors of cervical squamous intraepithelial lesions among HIV-infected women in Dar es Salaam, Tanzania.

    Science.gov (United States)

    Liu, Enju; McCree, Renicha; Mtisi, Expeditho; Fawzi, Wafaie W; Aris, Eric; Lema, Irene A; Hertzmark, Ellen; Chalamilla, Guerino; Li, Nan; Vermund, Sten H; Spiegelman, Donna

    2016-03-01

    To determine the prevalence and predictors of cervical squamous intraepithelial lesions (SIL) among HIV-infected women in Tanzania, a cross-sectional study was conducted among HIV-infected women at HIV care and treatment clinics. A Papanicolaou (Pap) smear was used as a screening tool for detection of cervical SIL. From December 2006 to August 2009, 1365 HIV-infected women received cervical screening. The median age was 35 (interquartile range [IQR]: 30-42) years, and the median CD4 + cell count was 164 (IQR: 80-257) cells/mm(3). The prevalence of cervical SIL was 8.7% (119/1365). In multivariate analysis, older age (≥50 versus 30-cervical inflammation (PR, 1.73; 95% CI, 1.16-2.60, p = 0.008) were associated with an increased risk of cervical SIL. Women with advanced WHO HIV disease stage (IV versus I/II: PR, 3.45; 95% CI, 1.35-8.85, p for trend = 0.01) had an increased risk for high-grade SIL. In resource-limited settings where it is not feasible to provide cervical cancer prevention services to all HIV-infected women, greater efforts should focus on scaling-up services among those who are older than 50 years, with lower CD4 cell counts and advanced HIV disease stage.

  1. Two-year clinical evaluation of three adhesive systems in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Evrim Eliguzeloglu Dalkilic

    2012-04-01

    Full Text Available OBJECTIVES: Adhesive systems are continuously being introduced to Dentistry, unfortunately often without sufficient clinical validation. The aim of this study was to evaluate the clinical performance of cervical restorations done with three different adhesive systems. MATERIAL AND METHODS: 158 non-carious cervical lesions of 23 patients were restored with a nanofilled composite resin (Filtek Supreme, 3M/ESPE combined with Single Bond (3M/ESPE, group SI, Clearfil SE (Kuraray Medical Inc., group CL and Xeno III (De Trey Dentsply, group XE. In groups SI-B, CL-B and XE-B, the outer surface of the sclerotic dentin was removed by roughening with a diamond bur before application of the respective adhesive systems. In groups CL-BP and XE-BP, after removal of the outer surface of the sclerotic dentin with the bur, the remaining dentin was etched with 37% phosphoric acid and the self-etch adhesive systems Clearfil SE and Xeno III were applied, respectively. Lesions were evaluated at baseline, and restorations after 3 months, 1 year and 2 years using modified USPHS criteria. RESULTS: After 2 years, no significant difference was found between the retention rates of the groups (p >0.05. Although groups CL and SI showed significantly better marginal adaptation than group XE (p0.05. After 2 years no significant difference was observed among the marginal staining results of all groups (p>0.05. CONCLUSION: Although all adhesive systems showed similar retention rates, Clearfil SE and Single Bond showed better marginal adaptation than Xeno III after 2 years of follow-up.

  2. Study on detection and treatment of cervical lesions in 10 212 women in Yingcheng%应城市10212例妇女宫颈病变检测及治疗的研究

    Institute of Scientific and Technical Information of China (English)

    刘永桥; 李艳; 杨丽

    2012-01-01

    Objective; To detect, diagnose, and evaluate cervical lesions, especially the degree and state of cervical precancerous lesion by liquid - based thin layer cytological test ( TCT) combined with biopsy, provide an accurate reference for treating cervical lesions reasonably in clinic, observe the curative effect. Methods; A total of 10 212 women were detected by TCT for successive three years, including 457 female workers from the hospital. Cytological diagnosis was made according to TBS criteria. Partial women with positive TCT results received cervical biopsy. Results; Among 10 212 women, 391 women were found with positive cytological results (above ASC - US) , the positive rate was 3. 83% , including 296 women with ASC - US (2. 90% ) , 85 women with ASC - H ( 0. 84% ) , 5 women with LSIU (0. 05% ) , 3 women with HSIL (0. 03% ) , one woman with SCC (0. 01% ) , and one woman with AGC (0. 01% ) . The positive rate of cytologieal screening in 457 female workers in the hospital for successive three years was 3.42% , 0. 88% , and 0.00% , respectively. Conclusion: TCT has distinct advantages for cervical lesions screening and curative effect observation after gynecological treatment , TCT combined with cervical biopsy and treatment can reduce the occurrence and development of cervical lesions, which has important clinical application value.%目的:采用液基薄层细胞制片技术(TCT)结合组织活检术检测、诊断和评价宫颈病变,特别是癌前病变的程度及所处的状态,为临床合理治疗宫颈病变提供准确依据,观察治疗后的效果.方法:运用TCT连续3年检测了10 212例妇女,包括457例应城市人民医院女职工.细胞学诊断按TBS标准.对TCT部分阳性者进行宫颈活检.结果:10 212例妇女中,细胞学阳性(ASC-UC以上)391例,阳性率为3.83%,其中ASC-UC 296例(2.90%)、ASC-H 85例(0.84%)、LSIL 5例(0.05%)、HSIL 3例(0.03%)、SCC 1例(0.01%)、AGC 1例(0.01%).应城市人民医院457

  3. Effects of glutamine and arginine on the gastric precancerous lesions in rats%精氨酸和谷氨酰胺对胃黏膜癌前病变影响的实验研究

    Institute of Scientific and Technical Information of China (English)

    江涛; 舒晓亮; 徐涵; 曾英

    2012-01-01

    Objective: To investigate the effect of glutamine and arginine on gastric precancerous in Sprague-Dawley rats. Methods: One-hundred rats were randomly divided into test A group (n = 20 ) , test B group ( n = 20 ) , test C group ( n = 20 ) , control group ( n = 20 ) , normal group ( n = 20). N-methyl-N-nitro-N-nitrosoguanidine(MNNG,100μg/mL) was used feeding rats to make animal model of gastric precancerous lesions. All rats were fed with Nutrison as base enteral nutrition. The rats in testA, testB and testC groups were added with glutamine +arginine, arginine and glutamine in Nutrison,respectively. After 8 weeks,all rats were killed. Immune function were measured. Expression of oncogenes were also determined. Histopathological changes of gastric mucosa were observed. Results: The levels of T-lymphocyte subsets CD4,the ratio of CD4/CD8, immunoglobulin A(IgA)and immunoglobulin M(IgM) were higher, but the levels of tumor necrosis factor(TNF-α) and interleukin-6(IL-6) were lower in test A, test B, test C groups than those in the control group. Compared with the control group, the expression of bcl-2, c-myc and the incidence of mild to severe metaplasia, dysplasia were significantly lower in test A, test B, test C groups . The expression of bcl-2,c-myc and the incidence of mild to severe metaplasia, dysplasia were lower in test A than those in test B, test C groups. There were not statistic difference among test B, test C groups. Compared with normal group,the levels of IgA,IgM, IgG,CD4 and the ratio of CD4/CD8 were lower, the levels of TNF-α,IL-6 and CD8 were significantly highter in control groups. The expression of bcl-2, c-myc and the incidence of mild to severe metaplasia, dysplasia were significantly higher in control group than those in normal group. Conclusion: Glutamine and arginine can regulate immune function and cell proliferation and effectively intervene in the development of gastric precancerous lesions in rats.%目的:研究精氨酸(Arg)和谷氨酰

  4. Precancerous Cervix in Human Immunodeficiency Virus Infected Women Thirty Years Old and above in Northern Uganda

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    Jonathan Izudi

    2016-01-01

    Full Text Available Background. Little is known about precancerous cervical lesion (PCCL, the precursor of cervical cancer among Human Immunodeficiency (HIV infected women in a postconflict setting of Northern Uganda. Objective. To establish factors associated with PCCL among HIV infected women above thirty years of age in a postconflict setting of Northern Uganda. Method. This retrospective cohort study used electronic data from 995 HIV-positive women that attended cervical cancer screening during June 2014 and December 2015. Data on social, sexual, obstetric, and gynecological factors was analyzed at 95% confidence level. Multivariate analysis determined factors independently associated with positive PCCL. Probability value less than 5% was considered significant. Results. Prevalence of PCCL was 3.0% (95% confidence interval (CI: 2.0–4.3. A positive PCCL was significantly associated with absence of sexually transmitted diseases (STDs during clinic visits (adjusted odds ratio, aOR = 0.24; 95% confidence interval (CI: 0.09–0.64; P=0.004 and first pregnancy before the age of 20 years (aOR = 3.09; 95% CI: 1.21–7.89; P=0.018. Conclusion. The prevalence of PCCL was low in the postconflict setting of Northern Uganda. HIV-positive women presenting with STDs and those with first pregnancy before the age of 20 years were at increased risk of PCCL.

  5. Precancerous Cervix in Human Immunodeficiency Virus Infected Women Thirty Years Old and above in Northern Uganda.

    Science.gov (United States)

    Izudi, Jonathan; Adrawa, Norbert; Amongin, Dinah

    2016-01-01

    Background. Little is known about precancerous cervical lesion (PCCL), the precursor of cervical cancer among Human Immunodeficiency (HIV) infected women in a postconflict setting of Northern Uganda. Objective. To establish factors associated with PCCL among HIV infected women above thirty years of age in a postconflict setting of Northern Uganda. Method. This retrospective cohort study used electronic data from 995 HIV-positive women that attended cervical cancer screening during June 2014 and December 2015. Data on social, sexual, obstetric, and gynecological factors was analyzed at 95% confidence level. Multivariate analysis determined factors independently associated with positive PCCL. Probability value less than 5% was considered significant. Results. Prevalence of PCCL was 3.0% (95% confidence interval (CI): 2.0-4.3). A positive PCCL was significantly associated with absence of sexually transmitted diseases (STDs) during clinic visits (adjusted odds ratio, aOR = 0.24; 95% confidence interval (CI): 0.09-0.64; P = 0.004) and first pregnancy before the age of 20 years (aOR = 3.09; 95% CI: 1.21-7.89; P = 0.018). Conclusion. The prevalence of PCCL was low in the postconflict setting of Northern Uganda. HIV-positive women presenting with STDs and those with first pregnancy before the age of 20 years were at increased risk of PCCL.

  6. Human papillomavirus types distribution in eastern Sicilian females with cervical lesions. A correlation with colposcopic and histological findings.

    Science.gov (United States)

    Le Donne, Maria; Giuffrè, Giuseppe; Caruso, Carmela; Nicotina, Piero Antonio; Alibrandi, Angela; Scalisi, Rosalba; Simone, Angela; Chiofalo, Benito; Triolo, Onofrio

    2013-07-01

    To determine human papillomavirus (HPV) types distribution in cervical lesions in a Southern Italian female population in Messina and their relationship between HPV type and grade of colposcopic and histopathological abnormality, a total of 253 women aged 17-68 years, with previous cytological abnormalities, were included in this study. HPV-DNA testing, colposcopy and biopsy were performed. For each sample, cervical cells were collected by centrifugation and DNA was extracted, followed by a PCR-based HPV-DNA assay and reverse dot blot genotyping. HPV-16 was found the most common type (46.6 %) followed by HPV-31 (26.9 %), -6 (18.6 %), -58 (8.8 %), -18 (6.7 %), -66 (5.7 %), -52 and -53 (4.7 %). Out of 62 women with abnormal transformation zone (ATZ) area compatible with squamous intraepithelial lesion (SIL) or cervical cancer (CC), 64.5 % was found high risk (HR) HPV-positive. Moreover the severity of the colposcopic diagnosis was positively correlated with the higher HPV oncogenicity risk (HPV-16 P = 0.023; and HPV-53 P = 0.047). The HPV-16 was found the most prevalent type within each histological category: 66.7 %, 31.2 %, 44 % and 37.2 % of CC, high grade (H)SIL, low grade (L)SIL and chronic cervicitis respectively; followed by HPV-31 present in 25 %, 8 %, and 13.3 % of HSIL, LSIL and chronic cervicitis respectively. A higher HPV incidence than the rest of Italy was found, in agreement with that detected by other authors for the South of the country. These data provide further information about the types prevalence in women with cervical lesions living in Eastern Sicily, suggesting the introduction of new targeted vaccines against a wider spectrum of HPV.

  7. [New paradigms and challenges in cervical cancer prevention and control in Latin America].

    Science.gov (United States)

    Almonte, Maribel; Murillo, Raúl; Sánchez, Gloria Inés; Jerónimo, José; Salmerón, Jorge; Ferreccio, Catterina; Lazcano-Ponce, Eduardo; Herrero, Rolando

    2010-01-01

    Cervical cancer continues to be a significant health problem in Latin America. The use of conventional cytology to detect precancerous cervical lesions has had almost no major impact on reducing cervical cancer incidence and mortality rates, which are still high in the region. The availability of new screening tools to detect precancerous lesions provide great opportunities for cervical cancer prevention in the region, as do highly efficacious HPV vaccines able to prevent nearly all lesions associated with HPV-16 and -18 when applied before viral exposure. This paper summarizes the scientific evidence and regional experiences related to: i) the use of HPV testing and visual inspection after the application of acetic acid (VIA) in primary screening and ii) the implementation of adolescent HPV vaccination programs. Finally, we outline a number of recommendations for different resource settings. The feasibility of implementing successful and sustainable national cervical cancer prevention programs in Latin American countries in the region will depend on health priorities and the availability of infrastructure and health personnel--as determined by rigorous local situational analysis.

  8. The application of HPV-DNA detection combined with VIA test in screening of precancerous lesion of uterine cervix%高危型HPV检测联合肉眼醋酸试验在宫颈癌前病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    赵金娟

    2012-01-01

    目的:研究在宫颈癌前病变筛查中采用高危型HPV检测联合肉眼醋酸试验的应用效果.方法:选取2007年8月~2010年2月于我院治疗的可疑宫颈癌前病变患者532例,应用5%醋酸和Lugol's 液涂抹于宫颈表面,筛选出有异常着色的患者320例,再采用实时荧光定量PCR技术检测高危型HPV病毒感染情况.结果:320例肉眼醋酸试验异常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染190例,阳性率为59.38%;212例肉眼醋酸试验正常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染18例,阳性率为8.49%.结论:肉眼醋酸试验联合高危型HPV检测可及时、有效地发现宫颈癌前病变,可作为宫颈癌前病变筛查方案应用于临床.%Objective: To study the efficiency of HPV-DNA detection combined with visual inspection with acetic acid (VIA) test in screening of precancerous lesions of uterine cervix. Methods: 532 suspected precancerous lesion of uterine cervix patients in our hospital from August 2007 to February 2010 were selected. 5% acetic acid and Lugol's liquid were painted on the surface of cervix of all patients, and 320 cases of patients were screened by the abnormal color. Then the re -al-time fluorescence quantitative polymerase chain reaction technology was used to test high risk HPV infection in all pa -tients. Results: High risk HPV infection test of 320 patients who were showed abnormal color in VIA test was showed that 190 cases got HPV positive infection, the positive rate was 59.38%. High risk HPV infection test of 212 patients who were showed normal color in VIA test was showed that 18 cases got HPV positive infection, the positive rate was 8.49%. Conclusion: VIA test combined with HPV-DNA checking can timely and effectively discover precancerous lesion, which can be used in clinic as precancerous lesion screening plan.

  9. Association between micronucleus frequency and cervical intraepithelial neoplasia grade in Thinprep cytological test and its significance.

    Science.gov (United States)

    Shi, Yong-Hua; Wang, Bo-Wei; Tuokan, Talaf; Li, Qiao-Zhi; Zhang, Ya-Jing

    2015-01-01

    A micronucleus is an additional small nucleus formed due to chromosomes or chromosomal fragments fail to be incorporated into the nucleus during cell division. In this study, we assessed the utility of micronucleus counting as a screening tool in cervical precancerous lesions in Thinprep cytological test smears under oil immersion. High risk HPV was also detected by hybrid capture-2 in Thinprep cytological test smears. Our results showed that micronucleus counting was significantly higher in high-grade squamous intraepithelial lesion (HSIL) and invasive carcinoma cases compared to low-grade squamous intraepithelial lesion (LSIL) and non-neoplastic cases. Receiver operating characteristic (ROC) curve analysis revealed that micronucleus counting possessed a high degree of sensitivity and specificity for identifying HSIL and invasive carcinoma. Cut-off of 7.5 for MN counting gave a sensitivity of 89.6% and a specificity of 66.7% (P = 0.024 and AUC = 0.892) for detecting HSIL and invasive carcinoma lesions. Multiple linear regression analysis showed that only HSIL and invasive cancer lesions not age, duration of marital life and number of pregnancy are significantly associated with MN counting. The positive rate of high risk HPV was distinctly higher in LSIL, HSIL and invasive cancer than that in non-neoplstic categories. In conclusions, MN evaluation may be viewed as an effective biomarker for cervical cancer screening. The combination of MN count with HPV DNA detection and TCT may serve as an effective means to screen precancerous cervical lesions in most developing nations.

  10. Garcinol对实验性口腔癌化学预防作用的研究%Chemopreventive effect of Garcinol on DMBA-induced oral precancerous lesion in hamsters

    Institute of Scientific and Technical Information of China (English)

    李芳; 孙正

    2011-01-01

    目的:探讨Garcinol对二甲基苯并蒽(7,12-dimethylbenz anthracene,DMBA)诱发的金黄地鼠口腔癌的化学预防作用.方法:30只金黄地鼠分3组:阴性对照组(6只)不涂药;阳性对照组(12只)涂0.5%DMBA于左侧颊囊,每周3次,共涂3周;Garcinol组(12只)前3周处理同阳性对照组,3周后换涂5 mmol/L Garcinol,每周3次,涂1周.第4周末实验结束处死所有地鼠,处死前2 h腹腔注射50 mg/kg的5-溴脱氧尿嘧啶(5-bromodeoxyuridine,BrdU).取左侧颊囊行组织病理学观察和BrdU免疫组化染色.结果:地鼠颊囊涂Garcinol 1周后,炎症细胞数/mm2由阳性对照组的158.65±26.51减少到55.03±22.80(P<0.01).单纯增生的病灶数由4.60±1.42减少到1.25±0.82(P<0.05).异常增生的病灶数由3.47±1.12减少到1.13±0.67(P<0.05).BrdU增殖指数由6.57±2.03减少到2.17±0.53(P<0.01).结论:Garcinol对DMBA诱发的金黄地鼠口腔癌前病变具有一定的化学预防作用,其机制可能与抑制炎症和细胞增殖有关.%Objective: To study the preventive effect of Garcinol on 7,12-dimethylbenz anthracene (DMBA) induced oral precancerous lesion in Golden Syrian hamsters. Methods: 30 hamsters were divided into three groups. 6 hamsters in health group were not treated, 24 were treated with 0.5% DMBA solution topically in the left cheek pouch three times per week for three consecutive weeks. After the last DMBA treatment ,the hamsters were divided into two groups at random. 12 animals in control group were not treated, 12 in treatment group received 5 mmol/L Garcinol three times within the following week. 4 weeks after the start of the experiment the animals were injected with 5-bromodeoxyuridine (BrdU) at 50 mg/kg and then sacrificed. The cheek pouch samples were collected for histopathology and BrdU immunohistochemisty. Results: Garcinol significantly decreased the number of inflammatory cells/mm2 in the lesion tissue from 158.65 ± 26. 51 to 52.25 ± 18.53 ( P < 0.01 ). The incidence of

  11. Incidence of cervical lesions in Danish women before and after implementation of a national HPV vaccination program

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Dehlendorff, Christian; Junge, Jette;

    2014-01-01

    PURPOSE: Approximately 7% of cervical cancers and about 50% of high-grade cervical precursor lesions are caused by human papillomavirus (HPV) types 16 and 18. Denmark introduced the quadrivalent HPV vaccine into the vaccination program for 12-year-old girls in 2009 supplemented by a first catch......+) and cervical intraepithelial neoplasia grade 2 or worse (CIN2+) increased in all age groups in 2000-2010. After introduction of the quadrivalent HPV vaccine into the vaccination program, the incidence of atypia+ decreased significantly in women younger than 18 years (EAPC -33.4%; 95% CI -49.6; -12.0) and in 18...... in age groups with high HPV vaccine coverage, indicating an early effect of HPV vaccination....

  12. Aberrant DNA methylation in cervical carcinogenesis

    Institute of Scientific and Technical Information of China (English)

    Hui-Juan Yang

    2013-01-01

    Persistent infection with high-risk types of human papillomavirus(HPV) is known to cause cervical cancer; however,additional genetic and epigenetic alterations are required for progression from precancerous disease to invasive cancer.DNA methylation is an early and frequent molecular alteration in cervical carcinogenesis.In this review,we summarize DNA methylation within the HPV genome and human genome and identify its clinical implications.Methylation of the HPV long control region (LCR) and L1 gene is common during cervical carcinogenesis and increases with the severity of the cervical neoplasm.The L1 gene of HPV16 and HPV18 is consistently hypermethylated in invasive cervical cancers and can potentially be used as a clinical marker of cancer progression.Moreover,promoters of tumor suppressor genes (TSGs) involved in many cellular pathways are methylated in cervical precursors and invasive cancers.Some are associated with squamous cell carcinomas,and others are associated with adenocarcinomas.Identification of methylated TSGs in Pap smear could be an adjuvant test in cervical cancer screening for triage of women with high-risk HPV,atypical squamous cells of undetermined significance,or low grade squamous intraepithelial lesion (LSIL).However,consistent panels must be validated for this approach to be translated to the clinic.Furthermore,reversion of methylated TSGs using demethylating drugs may be an alternative anticancer treatment,but demethylating drugs without toxic carcinogenic and mutagenic properties must be identified and validated.

  13. Differential methylation of E2 binding sites in episomal and integrated HPV 16 genomes in preinvasive and invasive cervical lesions.

    Science.gov (United States)

    Chaiwongkot, Arkom; Vinokurova, Svetlana; Pientong, Chamsai; Ekalaksananan, Tipaya; Kongyingyoes, Bunkerd; Kleebkaow, Pilaiwan; Chumworathayi, Bandit; Patarapadungkit, Natcha; Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-05-01

    Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation of the squamous epithelial cells at the uterine cervix. The HPV E2 protein is a key transcriptional regulator of the E6-E7 genes. It binds to four E2 binding sites (E2BSs 1-4) in the viral upstream regulatory region (URR). Modification of E2 functions, for example, by methylation of E2BSs is hypothesized to trigger enhanced expression of the viral E6-E7 oncogenes. In the majority of HPV-transformed premalignant lesions and about half of cervical carcinomas HPV genomes persist in an extra-chromosomal, episomal state, whereas they are integrated into host cells chromosomes in the remaining lesions. Here we compared the methylation profile of E2BSs 1-4 of the HPV 16 URR in a series of 18 HPV16-positive premalignant lesions and 33 invasive cervical cancers. CpGs within the E2BSs 1, 3, and 4 were higher methylated in all lesions with only episomal HPV16 genomes compared with lesions displaying single integrated copies. Samples with multiple HPV16 integrated copies displayed high methylation levels for all CpGs suggesting that the majority of multiple copies were silenced by extensive methylation. These data support the hypothesis that differential methylation of the E2BSs 1, 3 and 4 is related to the activation of viral oncogene expression in cervical lesions as long as the viral genome remains in the episomal state. Once the virus becomes integrated into host cell chromosomes these methylation patterns may be substantially altered due to complex epigenetic changes of integrated HPV genomes.

  14. A pooled analysis of continued prophylactic efficacy of quadrivalent human papillomavirus (Types 6/11/16/18) vaccine against high-grade cervical and external genital lesions

    DEFF Research Database (Denmark)

    Kjaer, Susanne K; Sigurdsson, Kristján; Iversen, Ole-Erik;

    2009-01-01

    Quadrivalent human papillomavirus (HPV) vaccine has been shown to provide protection from HPV 6/11/16/18-related cervical, vaginal, and vulvar disease through 3 years. We provide an update on the efficacy of the quadrivalent HPV vaccine against high-grade cervical, vaginal, and vulvar lesions bas...

  15. Cervical Lesions in Women Younger than 25 Years Lesiones de cuello uterino en mujeres menores de 25 años

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available

    Background: Cervical cancer is essentially a sexually transmitted disease related to human papillomavirus infection. This disease’s incidence has been globally enhanced, particularly in increasingly younger women. Objective: To determine cervical lesions in women younger than 25 years. Methods: An observational, descriptive and correlational study including 248 patients registered at the Pathological Anatomy Service of the General University Hospital Dr. Gustavo Aldereguía Lima of Cienfuegos was conducted from January to December 2007. It also included the first quarter of 2008. The following variables were analyzed: patient’s age, age at first intercourse, number of sexual partners, type and degree of injury, and reason for attending consultation. Results: Women over 21 years old and those who had had 2 and 3 sexual partners predominated. The main reason for attending consultation was cervicitis (84, 7%. In 22.6% of women grade I intraepithelial neoplasia was detected, while grade II and III were detected in 41, 9% and 33, 1%, respectively. In 90% of younger patients the lesion was malignant. Conclusions: The presence of cervical lesions in women under 25 years is not low and neither is the frequency of malignant lesions.

    Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a

  16. Cervical pre-malignant lesions in HIV infected women attending Care and Treatment Centre in a tertiary hospital, Dar es Salaam, Tanzania.

    Science.gov (United States)

    Balandya, Belinda S; Pembe, Andrea B; Mwakyoma, Henry A

    2011-09-01

    The aims of this study was to determine proportion of HIV infected women with cervical pre-malignant lesions; and compare the use of Visual Inspection of the cervix after application of Acetic acid (VIA) and Papanicolau (Pap) smear in screening for cervical premalignant lesions in HIV positive women attending Care and Treatment Centre (CTC) at Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. A total of 316 women aged 18-70 years had a Pap smear taken for cytology, followed by spraying onto the cervix with 4% acetic acid and then inspecting it. Cytology was considered negative when there was no Cervical Intraepithelial Neoplasia (CIN) lesion reported from the Pap smear taken, and positive if CIN lesion 1, 2 or 3 was reported. Detection of a well-defined, opaque acetowhite lesion close to the squamocolumnar junction or close to the external cervical os constituted a positive VIA. Out of 316 women, 132 women had acetowhite lesions on VIA, making the proportion of abnormal cervical lesions to be 42.4%. One hundred and one out of 312 women (32.4%) had CIN lesions detected on Pap smear. The proportion of agreement between these two tests was 0.3. The proportion of agreement was moderate in women with advanced WHO HIV clinical stage of the disease and in women not on ART (Anti Retroviral Therapy). Women with CD-4 count less than 200 cells/mm3 had more abnormal cervical lesions. There is considerable proportion of HIV positive women with premalignant lesions of the cervix. Considering the proportion of HIV women with abnormal lesions and the difficulty in logistics of doing Pap smear in low resource settings, these results supports the recommendation to introduce screening of premalignant lesions of the cervix using VIA to all HIV infected women.

  17. CCR2 and CCR5 genes polymorphisms in women with cervical lesions from Pernambuco, Northeast Region of Brazil: a case-control study.

    Science.gov (United States)

    Santos, Erinaldo Ubirajara Damasceno dos; Lima, Géssica Dayane Cordeiro de; Oliveira, Micheline de Lucena; Heráclio, Sandra de Andrade; Silva, Hildson Dornelas Angelo da; Crovella, Sergio; Maia, Maria de Mascena Diniz; Souza, Paulo Roberto Eleutério de

    2016-03-01

    Polymorphisms in chemokine receptors play an important role in the progression of cervical intraepithelial neoplasia (CIN) to cervical cancer (CC). Our study examined the association of CCR2-64I (rs1799864) andCCR5-Δ32 (rs333) polymorphisms with susceptibility to develop cervical lesion (CIN and CC) in a Brazilian population. The genotyping of 139 women with cervical lesions and 151 women without cervical lesions for the CCR2-64I and CCR5-Δ32 polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism. The individuals carrying heterozygous or homozygous genotypes (GA+AA) for CCR2-64I polymorphisms seem to be at lower risk for cervical lesion [odds ratio (OR) = 0.37, p = 0.0008)]. The same was observed for the A allele (OR = 0.39, p = 0.0002), while no association was detected (p > 0.05) with CCR5-Δ32 polymorphism. Regarding the human papillomavirus (HPV) type, patients carrying the CCR2-64Ipolymorphism were protected against infection by HPV type 16 (OR = 0.35, p = 0.0184). In summary, our study showed a protective effect ofCCR2-64I rs1799864 polymorphism against the development of cervical lesions (CIN and CC) and in the susceptibility of HPV 16 infection.

  18. 围绝经期宫颈病变所致阴道流血%Perimenopausal abnormal vaginal bleeding caused by cervical lesions

    Institute of Scientific and Technical Information of China (English)

    王登凤; 张国楠

    2012-01-01

    围绝经期妇女中,不规则阴道流血是一个最常见、也是最易被忽视的症状,宫颈病变是其主要原因之一,主要包括了宫颈炎、宫颈炎症相关疾病、宫颈上皮内瘤变、宫颈癌、宫颈肌瘤、宫颈结核、宫颈妊娠等,可以通过妇科查体、宫颈脱落细胞学检查、宫颈活检等简单手段在短时间内得到确诊.%For perimenopausal women, abnormal vaginal bleeding is the most common symptom, which is also the most neglected one. In various causes, cervical lesion is the one which can be diagnosed by some simple methods, e. g. gynecological examination , cervical cytology, and cervical biopsy. Cervical lesions in this review include: cervicitis, cervicitis related disease, cervical intraepithelial neoplasia, cervical cancer, cervical myoma, tuberculosis of cervix, cervical pregnancy, etc.

  19. 高危型HPV检测联合细胞学检查在宫颈病变中的意义%Significance of high - risk HPV test combined with cytological examination in cervical lesions

    Institute of Scientific and Technical Information of China (English)

    宋萌颖; 陈东梅

    2012-01-01

    found with squamous cell carcinoma (SCC) , accounting for 0. 30%. Among 266 cases with abnormal cytological smears (ASCUS and above), 162 cases were found with positive high -risk HPV, the infection rate of HPV was 60. 90%. The conditions of different types of high - risk HPV infection specimens showed that there was significant difference in HPV infection rate among ASCUS group, LSIL group, and HSIL group (P<0. 01) .Conclusion; High - risk HPV test combined with TCT used in screening of early cervical cancer and precancerous lesion of cervix improves the accuracy of screening for cervical lesions.

  20. Cervical glandular atypia associated with squamous intraepithelial neoplasia: a premalignant lesion?

    OpenAIRE

    Brown, L J; Wells, M

    1986-01-01

    Recent studies have described premalignant changes in the endocervical epithelium, but morphological criteria for the diagnosis of cervical glandular atypia of lesser severity than adenocarcinoma in situ have not been established. Adenocarcinoma in situ is often associated with cervical intraepithelial neoplasia (CIN). The endocervical mucosa in 105 cases of CIN grade III was evaluated and compared with that of 100 controls. Sixteen cases of cervical glandular atypia and one case of adenocarc...

  1. A STUDY ON CERVICAL PAP SMEAR EXAMINATION IN PATIENT LIVING WITH HIV

    Directory of Open Access Journals (Sweden)

    B M Jha

    2012-02-01

    Full Text Available Background: Decline in morbidity & mortality due to cervical cancer in developed countries can be mainly attributed to early detection of precancerous & cancerous lesions due to extensive screening programmeof cervical Pap smear examination. HPV infection is a known etiological agent for cervical cancer. HIV infected women are at higher risk of contracting HPV infection due to immune compromised status. Objective: Present study has been undertaken mainly to detect precancerous & cancerous lesions as well as inflammatory lesions in female patients living with HIV & to emphasize the fact that Pap smear examination should be established as a part of routine protocol for examination in these women. Methods: The study was carried out on 407 HIV infected females attending Integrated Counseling &Testing Centre of government institute. As controls, 200 females (not falling under high risk category, attending the Obstetrics& Gynecology OPD with various gynecological complaints were taken & results were compared. Results: Squamous cell abnormalities were found about four times high as compared to control group(Pvalue <0.05. High incidences of squamous cell abnormalities were noted in patients with high parity (parity three or more. Conlcusion: Regular gynecological examination including Pap smear examinations is highly recommended for HIV infected females.Pap smear examination is a simple, cheap, safe & practical diagnostic tool for early detection of cervical cancer in high risk population. [National J of Med Res 2012; 2(1.000: 81-84

  2. Association between bacterial vaginosis and cervical intraepithelial neoplasia: systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Evy Gillet

    Full Text Available OBJECTIVE: Bacterial vaginosis (BV, the most common vaginal disorder among women of reproductive age, has been suggested as co-factor in the development of cervical cancer. Previous studies examining the relationship between BV and cervical intra-epithelial neoplasia (CIN provided inconsistent and conflicting results. The aim of this study is to clarify the association between these two conditions. METHODS: A systematic review and meta-analysis were conducted to summarize published literature on the association between BV and cervical pre-cancerous lesions. An extensive search of electronic databases Medline (Pubmed and Web of Science was performed. The key words 'bacterial vaginosis' and 'bacterial infections and vaginitis' were used in combination with 'cervical intraepithelial neoplasia', 'squamous intraepithelial lesions', 'cervical lesions', 'cervical dysplasia', and 'cervical screening'. Eligible studies required a clear description of diagnostic methods used for detecting both BV and cervical pre-cancerous lesions. Publications were included if they either reported odds ratios (OR and corresponding 95% confidence intervals (CI representing the magnitude of association between these two conditions, or presented data that allowed calculation of the OR. RESULTS: Out of 329 articles, 17 cross-sectional and 2 incidence studies were selected. In addition, two studies conducted in The Netherlands, using the national KOPAC system, were retained. After testing for heterogeneity and publication bias, meta-analysis and meta-regression were performed, using a random effects model. Although heterogeneity among studies was high (χ(2 = 164.7, p<0.01, I(2 = 88.5, a positive association between BV and cervical pre-cancerous lesions was found, with an overall estimated odds ratio of 1.51 (95% CI, 1.24-1.83. Meta-regression analysis could not detect a significant difference between studies based on BV diagnosis, CIN diagnosis or study population

  3. Randomized clinical trial of four adhesion strategies in cervical lesions: 12-month results.

    Science.gov (United States)

    de Paula, Eloisa Andrade; Tay, Lidia Yileng; Kose, Carlos; Mena-Serrano, Alexandra; Reis, Alessandra; Perdigão, Jorge; Loguercio, Alessandro D

    2015-01-01

    The aim of this study was to evaluate the 6- and 12-month clinical performance of four adhesion strategies from the same manufacturer (Kerr) in non-carious cervical lesions (NCCLs) using two evaluation criteria. Thirty-five patients, with at least four NCCLs each, participated in this study. After samplesize calculation, 180 restorations were assigned to one of the following groups: OFL (Optibond FL), OSP (Optibond Solo Plus), XTR (Optibond XTR), and AIO (Optibond All-In-One). The composite resin Filtek Supreme Ultra (3M ESPE) was placed incrementally. The restorations were evaluated at baseline, after 6 months, and after 12 months, using both the FDI and the USPHS-modified criteria. Statistical analyses were performed with Friedman repeated measures, ANOVA by rank, and the McNemar test for significance in each pair (α=0.05). Six restorations (2 for OFL, 1 for OSP, 2 for XTR, and 1 for AIO) were lost at 12 months (P>0.05 for both evaluation criteria). Marginal staining was observed in seven restorations using the FDI criteria (P>0.05) and three restorations using the USPHSmodified criteria (P>0.05). Eight restorations (2 for OSP, 3 for XTR, and 3 for AIO) were classified as Bravo for marginal adaptation using the USPHSmodified criteria (P>0.05). However, 62 restorations (14 for OFL, 12 for OSP, 15 for XTR, and 21 for AIO) were classified as Bravo using the FDI criteria (P>0.05). The four adhesion strategies showed similar clinical retention at 6 and 12 months. The FDI evaluation criteria tend to be more sensitive than the USPHS-modified criteria.

  4. Correlation of Trefoil factor 3 expression in gastric carcinoma and precancerous lesions with angiogenesis%TFF3在胃癌、癌前病变中的表达及与血管生成的关系

    Institute of Scientific and Technical Information of China (English)

    王臣臣; 顾晓萌; 王妍; 黄山英

    2013-01-01

    目的 研究三叶因子3(TFF3)在胃癌及癌前病变中的表达情况,探讨其与胃癌的发生和血管生成的关系.方法 采用免疫组织化学方法检测26例浅表性胃炎、25例肠上皮化生、20例不典型增生、66例胃癌组织中TFF3的表达,用CD34标记血管内皮,计数微血管密度(MVD).结果 胃癌组TFF3蛋白表达低于肠上皮化生组、不典型增生组,3组之间差异无统计学意义(P>0.05),但均明显高于浅表性胃炎组(P<0.01);胃癌组MVD值明显高于其他3组(P<0.01),肠上皮化生组、不典型增生组间差异无统计学意义(P>0.05),但明显高于浅表性胃炎组(P<0.01),TFF3表达与胃癌淋巴结转移和TNM分期有关(P<0.05),TFF3表达与MVD呈显著正相关(r=0.672),阳性表达者的MVD值明显高于阴性表达者(P<0.01).结论 TFF3可能参与了胃癌发生发展、浸润转移和血管形成,其表达水平可作为早期预测胃癌发生发展和预后的有用指标.%Objective To investigate the expression of TFF3 in gastric carcinoma and precancerous lesions, and explore the correlation in gastric carcinogenesis and angiogenesis. Methods The expression of TFF3 was detected by immuno-histochemistry in 26 cases of superficial gastritis, 25 cases of intestinal metaplasia, 20 cases of atypical hyperplasia and 66 cases of gastric carcinoma. MVD was detected by immunohistochemistry using anti-CD34 antibody. Results The expression of TFF3 was lower in gastric carcinoma than in intestinal metaplasia and atypical hyperplasia, with higher expressions than that in superficial gastritis (P 0.05) , with higher expressions than that in superficial gastritis(P < 0.01). The expression of TFF3 was correlated with lymph node metastasis and TNM staging(P < 0.05). The expressions of TFF3 and MVD were positively correlated (r = 0. 672). MVD with positive expression of TFF3 was higher than that with negative expression (P< 0. 01). Conclusion TFF3 may play important roles in

  5. Cytologic follow up of Low-grade Squamous Intraepithelial Lesions in Pap smears after integrated treatment with antimicrobials followed by oral turmeric oil extract

    OpenAIRE

    Joshi, Jayashree Vinay; Jagtap, Sujata S.; Paradkar, Prajakta Hemant; Walwatkar, Priya; Paradkar, Hemant S.; Affandi, Zubair M.; Ashok D.B Vaidya

    2016-01-01

    Cervical cancer is preventable because the carcinogenesis is slow and there are opportunities to detect precancerous lesions by Papanicolaou (Pap) smears, colposcopy, or HPV DNA tests and to treat them by antimicrobials, surgery or cold CO2 vapourization. We have earlier reported on the chemopreventive potential of integrated treatment with antimicrobials therapy followed by a standardized oral Turmeric Oil (TO) extract upto 12 weeks in women who had persistent Low-grade Squamous Intra-epithe...

  6. Differential role of gene hypermethylation in adenocarcinomas, squamous cell carcinomas and cervical intraepithelial lesions of the uterine cervix.

    Science.gov (United States)

    Blanco-Luquin, Idoia; Guarch, Rosa; Ojer, Amaya; Pérez-Janices, Noemí; Martín-Sánchez, Esperanza; Maria-Ruiz, Sergio; Monreal-Santesteban, Iñaki; Blanco-Fernandez, Laura; Pernaut-Leza, Eduardo; Escors, David; Guerrero-Setas, David

    2015-09-01

    Cervical cancer is the third most common cancer in women worldwide. The hypermethylation of P16, TSLC-1 and TSP-1 genes was analyzed in squamous cell carcinomas (SCC), cervical intraepithelial lesions (CIN) and adenocarcinomas (ADC) of the uterine cervix (total 181 lesions). Additionally human papillomavirus (HPV) type, EPB41L3, RASSF1 and RASSF2 hypermethylation were tested in ADC and the results were compared with those obtained previously by our group in SCC. P16, TSLC-1 and TSP-1 hypermethylation was more frequent in SCCs than in CINs. These percentages and the corresponding ones for EPB41L3, RASSF1 and RASSF2 genes were also higher in SCCs than in ADCs, except for P16. The presence of HPV in ADCs was lower than reported previously in SCC and CIN. Patients with RASSF1A hypermethylation showed significantly longer disease-free survival (P = 0.015) and overall survival periods (P = 0.009) in ADC patients. To our knowledge, this is the first description of the EPB41L3 and RASSF2 hypermethylation in ADCs. These results suggest that the involvement of DNA hypermethylation in cervical cancer varies depending on the histological type, which might contribute to explaining the different prognosis of patients with these types of tumors.

  7. Epidemiology of Cervical Squamous Intraepithelial Lesions in HIV Infected Women in Kenya: a cross-Sectional Study.

    Science.gov (United States)

    Memiah, Peter; Makokha, Violet; Mbuthia, Wangeci; Kiiru, Grace Wanjiku; Agbor, Solomon; Odhiambo, Francesca; Ojoo, Sylvia; Mbizo, Justice; Muhula, Samuel; Mahasi, Gabriel; Biadgilign, Sibhatu

    2015-03-01

    Cervical cancer is the second most common cancer among women worldwide. Infection with the human immunodeficiency virus (HIV) and its related immunosuppression are associated with an increased risk of prevalent, incident, and persistent squamous intraepithelial lesions (SILs) of the cervix. The objective of the study was to describe the prevalence and predictors of high-risk HPV and cervical cancer to support the need for strengthening cervical cancer screening programs for HIV infected women in Kenya. A cross sectional study was conducted in a hospital in Central Kenya, Kiambu district. The study population constituted of HIV positive women attending the ART treatment clinic. A total of 715 HIV positive women initiated on Antiretroviral Therapy (ART) were enrolled in this study. About 359 (52.1%) were less than 40 years of age and 644 (90.3%) of the patients were widowed. About 642 (92.6%) of the HIV infected women were in follow-up period of ≥ 1 year. The outcome/prognosis of the patients undergoing ICC was 3 cured, 5 good and 4 poor respectively. In a multivariable ordinal logistic regression analysis showed that for a one-unit decrease of CD4, we expect 1.23 log odds of increasing the severity of cervical cancer (B = 1.23, P treatment, enrolling patients on HAART with higher CD4 counts is recommended to see the net effect of HAART response.

  8. Preprocessing: A Step in Automating Early Detection of Cervical Cancer

    CERN Document Server

    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Uterine Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, colposcopic images or cervigrams are acquired in raw form. They contain specular reflections which appear as bright spots heavily saturated with white light and occur due to the presence of moisture on the uneven cervix surface and. The cervix region occupies about half of the raw cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. Therefore we focus on the cervical borders, so that we have a geometric boundary on the relevant image area. Our novel technique eliminates the SR, identifies the region of interest and makes the cervigram ready for segmentation algorithms.

  9. Preprocessing for Automating Early Detection of Cervical Cancer

    CERN Document Server

    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Uterine Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, colposcopic images or cervigrams are acquired in raw form. They contain specular reflections which appear as bright spots heavily saturated with white light and occur due to the presence of moisture on the uneven cervix surface and. The cervix region occupies about half of the raw cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. Therefore we focus on the cervical borders, so that we have a geometric boundary on the relevant image area. Our novel technique eliminates the SR, identifies the region of interest and makes the cervigram ready for segmentation algorithms.

  10. Cervicitis

    Science.gov (United States)

    ... much growth of normal bacteria in the vagina (bacterial vaginosis) can also cause cervicitis. ... under a microscope (may show candidiasis , trichomoniasis , or bacterial vaginosis) Pap test Tests for gonorrhea or chlamydia Rarely, ...

  11. Quadrivalent HPV vaccine effectiveness against high-grade cervical lesions by age at vaccination: A population-based study.

    Science.gov (United States)

    Herweijer, Eva; Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim-Dahlström, Lisen

    2016-06-15

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006-2013 and aged 13-29 (n = 1,333,691) was followed for vaccination and histologically confirmed high-grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1-IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18-0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46-0.64) and 22% (IRR = 0.78, 95%CI = 0.65-0.93) for those initiating vaccination at ages 17-19, and at ages 20-29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long-term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer.

  12. Local immunosuppression induced by high viral load of human papillomavirus: characterization of cellular phenotypes producing interleukin-10 in cervical neoplastic lesions.

    Science.gov (United States)

    Prata, Thiago Theodoro Martins; Bonin, Camila Mareti; Ferreira, Alda Maria Teixeira; Padovani, Cacilda Tezelli Junqueira; Fernandes, Carlos Eurico dos Santos; Machado, Ana Paula; Tozetti, Inês Aparecida

    2015-09-01

    A specific immune response to human papillomavirus (HPV) in the cervical microenvironment plays a key role in eradicating infection and eliminating mutated cells. However, high-risk HPVs modulate immune cells to create an immunosuppressive microenvironment, and induce these immune cells to produce interleukin 10 (IL-10). This production of IL-10, in conjunction with HPV infection, contributes to the appearance of cervical neoplastic lesions. We sought to characterize the IL-10-producing cellular phenotype, and investigate the influence of host and HPV factors upon the induction of an immunosuppressive microenvironment. Immunohistochemical analysis demonstrated an increase in IL-10 production by keratinocytes, macrophages and Langerhans cells in high-grade cervical lesions and cervical cancer. This increase was more pronounced in patients older than 30 years, and was also correlated with high viral load, and infection with a single HPV type, particularly high-risk HPVs. Our results indicate the existence of a highly immunosuppressive microenvironment composed of different IL-10-producing cellular phenotypes in cervical cancer samples, and samples classified as high-grade cervical lesions (cervical intraepithelial neoplasia stages II and III). The immunosuppressive microenvironment that developed for these different cellular phenotypes favours viral persistence and neoplastic progression.

  13. Cervical myelopathy due to single level disc herniation presenting as intramedullary mass lesion: What to do first?

    Directory of Open Access Journals (Sweden)

    Murat Sakir Eksi

    2015-01-01

    Full Text Available Cervical myelopathy (CM is mostly a degenerative process ending in myelopathic and/or radiculopathic syndromes. On T2-weighted magnetic resonance imaging (MRI, CM appears as a hyperintense area near the spondylotic spine. This high intensity signal depends on the impact of outer forces and their duration. It also determines the prognosis of the surgical candidate. A 40-year-old male patient admitted to our clinic with right upper extremity weakness and hypoesthesia that had started 2 months earlier. On neurological examination there was 2/5 motor weakness of right biceps brachii, and hypoesthesia over right C6 dermatome. Right upper extremity deep tendon reflexes were hypoactive, but lower ones were hyperactive. After clinical and radiological work-up, preliminary diagnosis was directed to a spinal intramedullary tumor. Total resection of the herniated cervical disc fragment and the mass lesion was managed. Pathology of the mass lesion was compatible with subacute infarct tissue and inflammatory response. Final diagnosis was CM under effect of cervical disc herniation. Contrast-enhanced spinal cord myelopathic lesions are very rare and resemble much more tumors and inflammatory processes. However, the principal treatment approach totally differs depending on pathology. When there are both a disc herniation and a high clinical suspicion; biopsy should be delayed. The most probable solution will be surgery for the disc disease with thorough preoperative scanning of vascular malformations; clinical and radiological close follow-up after surgery. Biopsy or surgical resection can be performed if patient deteriorates despite the primary surgery.

  14. TCT联合DNA定量细胞学检查对宫颈病变临床诊断价值研究%The study of TCT and DNA quantitive cytology in the diagnosis of cervical lesions.

    Institute of Scientific and Technical Information of China (English)

    刘桂依; 黄艳美

    2011-01-01

    cervical cytology, DNA quantitative cytology and pathology can provide early detection of cervical cancer and precancerous lesions.

  15. Prevalence of Human Papillomavirus Types Among Mexican Women with Intraepithelial Lesions and Cervical Cancer: Detection with MY09/MY011 and GP5+/GP6+ Primer Systems

    OpenAIRE

    Gloria Fernández-Tilapa; Berenice Illades-Aguiar; Dinorah Nashely Martínez-Carrillo; Luz Del Carmen Alarcón-Romero; Amalia Vences-Velázquez; Marco Antonio Terán-Porcayo; Elba Reyes-Maldonado; María Lilia Domínguez López; Ethel García-Latorre

    2007-01-01

    Squamous cervical carcinoma (SCC) is the most common cancer found in Mexican women. Human papillomavirus (HPV) infection is a prerequisite for this disease. In Mexico little is known about the prevalence of HPV-types and knowledge of the circulating genotypes by region is limited. The aim of this study was to determine the prevalence and genotypes of HPV in biopsies from women with intraepithelial lesions and SCC. A total of 211 cervical biopsies were studied. Histopathological analysis was d...

  16. Quality of life in women with cervical precursor lesions and cancer:a prospective, 6-month, hospital-based study in China

    Institute of Scientific and Technical Information of China (English)

    Zhi-Mei Zhao; Xiong-Fei Pan; Si-Han Lv; Yao Xie; Shao-Kai Zhang; You-Lin Qiao; Xiao-Rong Qi; Chun-Xia Yang; Fang-Hui Zhao

    2014-01-01

    The overal survival of patients with cervical cancer has improved due to detection at an early stage and availability of comprehensive treatments in China. As patients’ lives prolonged, it is important to understand their health-related quality of life (QoL) during and after treatment. We used the EQ-5D questionnaire to assess QoL of 194 patients with cervical lesions at Sichuan University West China Second Hospital between May 2010 and January 2011. Patients were surveyed before primary treatment and at 1, 3, and 6 months after primary treatment. Results showed a consistent decline in EQ-5D scores in the spectrum of cervical lesions at each time point after treatment (alP < 0.05). For patients with precursor lesions, there was an increasing trend along the timeline of treatment (P < 0.01). For patients with early-stage cervical cancer, EQ-5D scores declined in the first month (P = 0.01) and gradualy increased to higher levels at 6 months post-treatment than those before treatment (P < 0.01). EQ-5D scores followed a similar trend in patients with advanced cervical cancer (P = 0.04), though they did not statisticaly rebound after 6 months (0.84± 0.19 vs. 0.86± 0.11,P = 0.62). Regarding advanced cervical cancer, EQ-5D scores for women above 40 years of age appeared to recover more rapidly and reached higher levels than those for women below 40 years (P = 0.03). Caution and extra care are recommended in the early period of cervical cancer treatment given the slight deterioration in the QoL, and in particular, for younger cervical cancer patients. Our study implies that health care providers may need to improve the health-related QoL of cervical cancer patients.

  17. 醋酸-靛胭脂染色在基层医院早期胃癌及癌前病变诊断中的应用%Application of acetic acid-indigo carmine dyeing in diagnosis of early gastric cancer and precancerous lesions in basic level hospital

    Institute of Scientific and Technical Information of China (English)

    王伟强; 李晓红; 宋国平; 贾长龙; 鹿志军; 李淼; 成伟丽

    2014-01-01

    Objective To explore the approaches to improve the detection of early gastric and precancerous lesions for basic level hospi-tals. Methods The 72 patients with abnormal gastric mucosa observed by gastroscope arranged with pathology after acetic acid-indigo car-mine dyeing were considered as the dyeing group, and 68 patients with abnormal gastric mucosa observed by gastroscope directly arranged with pathology were considered as the control group. The dyeing conditions of gastric mucosa were observed and compared to pathology detec-tion. The detection rate of early gastric cancer and precancerous lesions in the two groups were compared. Results After acetic acid-indigo carmine dyeing, there were 16. 7% of demonstrated discoloration, 63. 9% of poor dyeing, and 14. 3% of even dyeing. The detection rate of early gastric cancer and high grade intraepithelial neoplasia in patients with mucosa discoloration (91. 7%) was obviously higher than that in patients with poor dyeing (8. 6%) or even dyeing (0. 0%). The detection rate of low grade intraepithelial neoplasia or intestinal metaplasia in patients with poor dyeing (82. 6%) was obviously higher than that in patients with mucosa discoloration (8. 3%) or even dyeing (14. 3%). The detection rate of early gastric cancer and precancerous lesions in dyeing group (13. 9%,63. 9%) was obviously higher than that in control group (2. 9%,29. 4%). Conclusion The acid-indigo carmine dyeing could increase the diagnosis of early gastric cancer and precancerous lesions in basic level hospital. It is adaptable to extend approach in basic level hospital for its low cost and simple operation.%目的:探讨提高基层医院早期胃癌及癌前病变检出率的方法。方法经胃镜检查发现胃黏膜异常进行醋酸-靛胭脂染色后取活检病理检查患者72例为染色组,同期胃镜下发现黏膜异常患者直接取组织活检病理检查患者68例为对照组,观察胃黏膜染色情况并与病理

  18. p27蛋白在牙龈癌和癌前病变组织中的表达及临床病理学意义%The expression of p27 protein in gingival cancer and precancerous lesions tissues and its clinical pathological significance

    Institute of Scientific and Technical Information of China (English)

    闫威; 陈勇; 李浩博

    2013-01-01

    目的探讨细胞周期相关蛋白p27在牙龈癌及口腔黏膜癌前病变组织中的表达及其临床病理学意义。方法应用SP免疫组化法检测21例正常黏膜组织、26例癌前病变(白斑、红斑)组织及46例牙龈癌组织中p27蛋白的表达水平,分析p27蛋白与牙龈癌的病理分级和临床分期的关系。结果 p27蛋白在正常口腔黏膜组织与癌前病变及牙龈癌组织中的阳性率和阳性表达率差异有统计学意义(P <0构.05),在正常口腔黏膜组织中的表达高于另2组p27蛋白的表达与患者性别、年龄等因素无相关性( P >0.05),但与病变的临床分级及病理分期密切相关( P <0.05)。结论 p27蛋白与癌前病变和牙龈癌的发生发展有密切关系,并与牙龈癌的临床分期成反比关系,与牙龈癌的病理分级成正比关系。%Objective To investigate the expression of cell cycle related protein p 27 in gingival cancer and oral precancerous lesions tissues as well its clinicopathological significance .Methods The experimental levels of p27 protein were detected by immunohistochemistry ( SP) in 21 cases of normal mucous membrane tissues ,26 cases of oral precancerous lesions tissues ( leukoplakia , erythema ) and 46 cases of gingival cancer tissues .The correlation between p27 protein and pathological classification , clinical stage of gingival cancer was analyzed . Results There were significant differences in the positive rates and positive expression rates among normal mucous membrane tissues,precancerous lesions tissues and gingival cancer tissues ( P 0.05),however,which were closely correlated with clinical stage and pathological classification of gingival cancer ( P <0.05).Conclusion The p27 protein is closely related to the pathogenesis and development of precancerous lesions and gingival cancer , which is negatively correlated with clinical stage ,however ,which is positively related to pathological

  19. LEEP in the Treatment of Cervical Lesions in 218 Cases of Clinical Observation%LEEP治疗宫颈病变218例临床观察

    Institute of Scientific and Technical Information of China (English)

    刘淑芬

    2013-01-01

      目的:观察 LEEP 治疗宫颈病变的临床疗效.方法:对本院妇科门诊发现的各种宫颈病变218例患者,采用 LEEP 宫颈环形电切术诊治,切除组织全部送病检.结果:术后2个月慢性宫颈炎96.43%完全愈合,宫颈湿疣100%愈合,CIN Ⅰ~Ⅱ100%愈合,CIN Ⅲ46.15%愈合,2例持续 CIN Ⅲ行全子宫切除术.结论:LEEP 宫颈环形电切术是目前诊治宫颈病变的理想方法,对早期诊断宫颈原位癌具有较高的临床价值,能有效阻断原位癌病变发展为浸润癌.%Objective:To observe on LEEP in treatment of cervical disease clinical curative effect.Method:The gynecological clinic was found in the 218 cases of various types of cervical lesion patients,using LEEP cervical loop electrosurgical excision procedure for cervical disease diagnosis and treatment,the removal of the tissue were all sent to the pathology examination.Result:2 months later,chronic cervicitis patients 96.43% achieved complete healing,cervical condyloma acuminatum lesions 100% patients achieved healing,cervical intraepithelial neoplasia Ⅰ~ Ⅱ 100% patients achieved healing,cervical intraepithelial neoplasia Ⅲ 46.15% patients achieved healing,2 cases because of persistent cervical intraepithelial neoplasia lesions Ⅲ for total hysterectomy. Conclusion:LEEP is currently the diagnosis and treatment of cervical lesions in the ideal method,for the early diagnosis of cervical carcinoma in situ has high clinical value,can effectively block the carcinoma in situ lesions progress to invasive cancer.

  20. Prevalence of high-risk human papillomavirus infection in different cervical lesion among organized health-examination women in Shanghai, China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Wen-ying; XUE Yue-zhen; CHEN Min; HAN Ling; LUO Man

    2008-01-01

    Background Cervical cancer is the second most common cause of death from cancer among women worldwide.Human papillomavirus(HPV)plays a central role in the etiology of cervical cancer.It is important to describe the prevalence of HPV infection in different types of cervical lesions and to explore the relation between HPV viral load and the severity of cervical lesions.Methods To describe the HPV infection prevalence and viral load in different age groups,we retrospectively investigated 6405 cases of women who were organized by their units to take health-examination.They were given Hybrid Capture ll tests between Janury 2005 and December 2006.The correlation between HPV viral load and pathology was assessed.Results Overall HPV infection prevalence was 29.1%(1864/6405),while in women 18-20 years old it was 54.4% (31/57),the highest among all age groups.After declining rapidly,HPV prevalence stabilized at about 30.0%in women aged 30 and older.Of the 6405 women,1 483 women had a colposcopic biopsy and 33.2%(492/1 483)were positive for HPV DNA.Twenty-one percent of women with a normal diagnosis(238/1 095)had HPV infection,a statistically significantly lower prevalence than in women with cervical lesions,including those with cervical intraepithelial neoplasia (68.8%in CINl,66.7% in CIN2,and 76.5%in CIN3)or with cervical cancer(94.1%).The correlation coefficient between viral load and cervical lesion severity was 0.134,which was not tatistically significant(P=0.075).Viral load values in women with CINs and cervical cancer were calculated,and no significant differences were dentified.Conclusions The revalence of high-risk HPV infection among women attending hospitals for health-examination in Shanghai is similar to the worldwide rate.HPV viral load can distinguish ervical lesions from normal individuals but cannot adequately predict the severity of cervical lesions.

  1. Clinical value of liquid-based thinPrep cytology test and cervical biopsy in the diagnosis of cervical Squamous epithelial lesions%液基薄层细胞学检测联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    赵瑞皎; 石曼丽; 张家兴; 孔令非

    2012-01-01

    exclude high grade intraepithelial lesions (ASC-H), 92 cases of low grade squamous intraepithelial lesions (LSIL), 80 cases of high grade squamous intraepithelial lesion (HSIL), and 1 5 cases of squamous cell carcinoma (SCC). There were 202 cases of cervical intraepithelial neoplasia (CIN)- 1, 22 cases of CIN-Ⅱ and CIN-Ⅲ, and 1 case of SCC among 1423 cases of ASC-US; and 12 cases of CIN- 1, 101 cases of CIN-Ⅱ and CIN-Ⅲ, and 5 cases of SCC among 214 cases of ASC-H. The consistent rates of diagnosis between TCT and colposcopy-directed biopsy were 63.04% (58/92) for LSIL, 81.25% (65/80) for HSIL, and 100% (15/15) for SCC, respectively. The consistent rates for SCC and HSIL were both higher than those for LSIL (P < 0.01). Conclusion: The high consistent rate of diagnose between TCT and colposcopy-directed biopsy suggests that the combination of these two diagnostic methods can improve the diagnostic rates of cervical cancer and precancerous lesions.

  2. Chlamydia infection in patients with and without cervical intra-epithelial lesions tested by real-time PCR vs. direct immunofluorescence.

    Science.gov (United States)

    Oliveira, Micheline de Lucena; Amorim, Melania Maria Ramos de; Souza, Paulo Roberto Eleutério de; Albuquerque, Lúcia Cristina Bezerra de; Brandão, Lucas André Cavalcanti; Guimarães, Rafael Lima

    2008-08-01

    This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia infection was 47.1% by direct IMF and 58.6% by real time PCR. A significant association was observed between Chlamydia diagnosis and presence of intra-epithelial cervical lesions, with about 80% positive results by direct IMF and 77.1% by real time PCR. However, the detected rate of infection with Chlamydia trachomatis was significantly greater in patients without intra-epithelial cervical lesions tested by real time PCR (40%) when compared to direct IMF (14.3%). The concordance between the tests was weak, with a kappa coefficient of 0.4. Both real time PCR and direct IMF detected elevated rates of Chlamydia infection in patients with intra-epithelial cervical lesions (80%) but the tests were discordant when patients without cervical lesions were tested, possibly because sensitivity of real time PCR is greater.

  3. 高危HPV病毒第2代杂交捕获法检测应用于宫颈癌以及癌前病变检查%Application of Hybrid Capture 2 for High-Risk Human Papillomavirus Testing in Cervical Cancer and Premalignant Lesion

    Institute of Scientific and Technical Information of China (English)

    张玉梅; 曹维克

    2011-01-01

    Objective: To explore the clinical value of hybrid capture 2 (hc2) in detection of high risk HPV in cervical cancer and pre-cancerous cervical lesions. Methods: Cervix exfoliated cells were detected with the second generation of hybrid capture hc2 method, and the diagnostic results such as specificity, sensitivity, accuracy, and pedicted value were compared with that of pathological diagnosis respectively. Results: The infection rate of HPV assayed by hc2 was 43. 8% in atypical squamous cell of undetermined signification (ASCUS), 7. 8% in ASCUS-H, 9. 7% low-grade squamous intraepithelial lesion(LSIL), and 28. 7% in high-grade squamous intraepithelial lesion (HSIL) respectively according to TBS diagnosis and classification system. For high grade-squ-mous intraepithelial lesion≥(CIN II ), the specificity, sensitivity, positive-predictive value and negative-predictive value by hc2 were 66. 7% , 93. 4% , 60. 0% , and 95. 0% respectively. In high virus load assay, there was significant difference between cervical cancer and CIN I (P<0. 01), and between cervical cancer/CIN and NILM group (P<0. 01), but there was no difference be-tween CIN and cervical cancer. Conclusion; The high-risk HPV test with hc2 is still a good method in diagnosing cervical cancer and premalignant lesion. Examining the high-risk HPV viral load with hc2 is related to cervical cancer and premalignant lesions, but not related to the severity of cervical lesions.%目的:探讨高危人类乳头瘤病毒(HPV)第二代杂交捕获实验(hc2)检测应用于宫颈癌以及癌前病变检查中的临床价值.方法:用hc2检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,对其特异度、敏感度、准确性、预测值等检测并分析.结果:hc2法检测高危型HPV的感染状况为无明确意义的非典型细胞的改变(ASCUS)44.7%,不典型鳞状细胞不除外高度病变(ASCUS-H)7.8%,低度鳞状上皮内病变(LSIL)19.7%,高

  4. Clinical Value of Endoscopic Esophageal Iodine Staining in the Early Diagnosis and Treatment of Esophageal Cancer and Precan-cerous Lesions%内镜下食管碘染在早期食管癌及癌前病变诊治中的临床应用价值

    Institute of Scientific and Technical Information of China (English)

    秦兴亚

    2015-01-01

    Objective To analyze the clinical value of endoscopic iodine dye in the early diagnosis and treatment of esophageal cancer and precancerous lesions .Methods Selecting 180 cases as the object , doing iodine endoscopic inspection process dyed checks on esopha-geal lesions in 50 cases, doing detailed records under the lesion morphology , location and size, and doing analysis and comparison on multi-ple biopsies and the esophageal squamous epithelium colored concrete results with the corresponding clinical and pathological examination . Results In 50 patients before receiving iodine dye check , 63 lesions points are suspected .In 40 cases after iodine staining dye -free area and lightly staining , a total of 58 points are found , which is about 1/3 of cases have ≥2 points with no staining area and lightly stained area . Conclusion Early diagnosis and treatment of esophageal cancer and precancerous lesions appear within the application in endoscopic has i -deal iodine dye detection results , with higher rate of clinical examination .So it is worth promotion and application .%目的:分析内镜下食管碘染在早期食管癌及癌前病变诊治中的临床应用价值。方法选取180例患者作为此次的研究对象,对50例经内镜检查有食管黏膜可能病变者给予碘染色检查,详细记录病灶的形态、位置以及大小等,行多处活检并把食管鳞状上皮组织具体的着色结果同相应的病理临床检查相互对比分析。结果50例患者接受碘染检查前怀疑病变点共63处,行碘染后40例出现无染色区以及淡染区共58处,约1/3病例出现≥2处无染色区以及淡染区。结论对早期食管癌以及癌前出现病变患者应用内镜下食管碘染色检查,可获得较为理想的检出效果,临床检诊率较高,值得在临床推广和应用。

  5. Evaluación de factores asociados a lesiones del tercio cervical de los elementos dentarios y su relación con el periodonto.

    OpenAIRE

    Busleiman, Federico José

    2015-01-01

    El tercio cervical dentario, el cual posee una íntima relación con los tejidos periodontales de sostén y protección, puede ser zona de asiento de lesiones cervicales cariosas (LCC), no cariosas (LCNC) y lesiones cervicales mixtas (LCM). OBJETIVO: identificar la frecuencia y características clínicas de cada tipo de lesión del tercio cervical, y su multicausalidad, a fin de establecer un patrón de factores de riesgo y la relación con tejidos periodontales. MATERIALES Y MÉTODOS: se realizó un es...

  6. Low NKp30, NKp46 and NKG2D expression and reduced cytotoxic activity on NK cells in cervical cancer and precursor lesions

    Directory of Open Access Journals (Sweden)

    Bravo-Cuellar Alejandro

    2009-06-01

    Full Text Available Abstract Background Persistent high risk HPV infection can lead to cervical cancer, the second most common malignant tumor in women worldwide. NK cells play a crucial role against tumors and virus-infected cells through a fine balance between activating and inhibitory receptors. Expression of triggering receptors NKp30, NKp44, NKp46 and NKG2D on NK cells correlates with cytolytic activity against tumor cells, but these receptors have not been studied in cervical cancer and precursor lesions. The aim of the present work was to study NKp30, NKp46, NKG2D, NKp80 and 2B4 expression in NK cells from patients with cervical cancer and precursor lesions, in the context of HPV infection. Methods NKp30, NKp46, NKG2D, NKp80 and 2B4 expression was analyzed by flow cytometry on NK cells from 59 patients with cervical cancer and squamous intraepithelial lesions. NK cell cytotoxicity was evaluated in a 4 hour CFSE/7-AAD flow cytometry assay. HPV types were identified by PCR assays. Results We report here for the first time that NK cell-activating receptors NKp30 and NKp46 are significantly down-regulated in cervical cancer and high grade squamous intraepithelial lesion (HGSIL patients. NCRs down-regulation correlated with low cytolytic activity, HPV-16 infection and clinical stage. NKG2D was also down-regulated in cervical cancer patients. Conclusion Our results suggest that NKp30, NKp46 and NKG2D down-regulation represent an evasion mechanism associated to low NK cell activity, HPV-16 infection and cervical cancer progression.

  7. Quadrivalent HPV vaccine effectiveness against high‐grade cervical lesions by age at vaccination: A population‐based study

    Science.gov (United States)

    Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim‐Dahlström, Lisen

    2016-01-01

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006–2013 and aged 13–29 (n = 1,333,691) was followed for vaccination and histologically confirmed high‐grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1‐IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18–0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46–0.64) and 22% (IRR = 0.78, 95%CI = 0.65–0.93) for those initiating vaccination at ages 17–19, and at ages 20–29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long‐term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer. PMID:26856527

  8. Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis

    Science.gov (United States)

    Wilting, Saskia M.; Verlaat, Wina; Jaspers, Annelieke; Makazaji, Nour A.; Agami, Reuven; Meijer, Chris J.L.M.; Snijders, Peter J.F.

    2013-01-01

    Deregulated expression of microRNAs (miRNAs) is common and biologically relevant in cervical carcinogenesis and appears only partly related to chromosomal changes. We recently identified 32 miRNAs showing decreased expression in high-grade cervical intraepithelial neoplasia (CIN) and carcinomas not associated with a chromosomal loss, 6 of which were located within a CpG island. This study aimed to investigate to what extent these miRNAs are subject to DNA methylation-mediated transcriptional repression in cervical carcinogenesis.   Methylation-specific PCR (MSP) analysis on a cell line panel representing different stages of human papillomavirus (HPV) induced transformation revealed an increase in methylation of hsa-miR-149, -203 and -375 with progression to malignancy, whereas expression of these miRNAs was restored upon treatment with a demethylating agent. All three miRNAs showed significantly increased levels of methylation in cervical carcinomas, whereas methylation levels of hsa-miR-203 and -375 were also significantly increased in high-grade CIN. A pilot analysis showed that increased hsa-miR-203 methylation was also detectable in HPV-positive cervical scrapes of women with high-grade CIN compared with controls. Similar to recent findings on hsa-miR-375, ectopic expression of hsa-miR-203 in cervical cancer cells decreased both the proliferation rate and anchorage independent growth. We found evidence for methylation-mediated transcriptional repression of hsa-miR-149, -203 and -375 in cervical cancer. Methylation of the latter two was already apparent in precancerous lesions and represent functionally relevant events in HPV-mediated transformation. Increased hsa-miR-203 methylation was detectable in scrapes of women with high-grade CIN, indicating that methylated miRNAs may provide putative markers to assess the presence of (pre)cancerous lesions. PMID:23324622

  9. Evaluation of 'see-see and treat' strategy and role of HIV on cervical cancer prevention in Uganda

    Directory of Open Access Journals (Sweden)

    Sandin Sven

    2010-05-01

    Full Text Available Abstract Background There is scant information on whether Human Immunodeficiency Virus (HIV seropositivity has an influence on the outcome of treatment of precancerous cervical lesions using cryotherapy. We studied the prevalence of cervical abnormalities detectable by visual inspection and cervical lesions diagnosed by colposcopy according to HIV serostatus and described the outcomes of cryotherapy treatment. Methods Trained nurses examined women not previously screened for cervical cancer using visual inspection with acetic acid (VIA and Lugol's iodine (VILI in two family planning/post natal clinics in Kampala, Uganda, from February 2007 to August 2008. Women with abnormal visual inspection findings were referred for colposcopic evaluation and HIV testing. Women with precancerous cervical lesions detected at colposcopy were treated mainly by cryotherapy, and were evaluated for treatment outcome after 3 months by a second colposcopy. Results Of the 5 105 women screened, 834 presented a positive screening test and were referred for colposcopy. Of these 625 (75% returned for the colposcopic evaluation and were tested for HIV. For the 608 (97.5% women in the age range 20-60 years, colposcopy revealed 169 women with cervical lesions: 128 had inflammation, 19 had low grade squamous intraepithelial lesion (LGSIL, 13 had high grade squamous intraepithelial lesion (HGSIL, 9 had invasive cervical cancer and 2 had inconclusive findings. Detection rates per 1 000 women screened were higher among the older women (41-60 years compared to women aged 20-40 years. They were accordingly 55% and 20% for inflammation, 10% and 2% for LGSIL, 5% and 2% for HGSIL, 6% and 1% for invasive cervical cancer. Of the 608 women, 103 (16% were HIV positive. HIV positivity was associated with higher likelihood of inflammation (RR = 1.7; 95% CI: 1.2-2.4. Conclusions Detection rates were higher among older women 41-60 years. Visual inspection of the cervix uteri with acetic acid

  10. Efficacy in treatment of cervical HRHPV infection by combination of beta interferon, and herbal therapy in woman with different cervical lesions.

    Science.gov (United States)

    Iljazović, Ermina; Ljuca, Dzenita; Sahimpasić, Ademir; Avdić, Silvija

    2006-11-01

    Cervical dysplasia, a premalignant lesion that can progress to cervical cancer, is caused primarily by a sexually transmitted infection with an oncogenic strain of the human papillomavirus (HPV). The HPV infections are treated through destroying the clinical lesions: laser, cryotherapy, podophyllin... The hope is that by causing local tissue inflammation that the body will be stimulated to mount an antibody response and thereby prevent recurrence. In contrast to other prevention approaches, vaccines can reduce susceptibility in uninfected partners by stimulating the immune system. Aloe vera has also been reported to retard tumour growth and stimulate the immune response to viruses. A list of possible actions of propolis includes: antibacterial, antifungal, antiviral, antioxidant, anticarcinogenic, antithrombotic and immunomodulatory. Research on the possible role of some B vitamins in preventing cancer began in the last few decades, but however this complex have an influence on immune status. The aim of our study is to try to treat the HPV infection as confirmed cause of neoplastic transformation with some herbal therapy and interferon and to try define the guidelines in the management of the HPV positive patients. Goal of this paper is to search for evidence of efficacy of any treatment for HPV infection of the cervix mostly in woman with no concomitant CIN. Fifty five woman affected by HPV genital infection were enrolled in the study from September 2005 to April 2006. Patients were classified according to the results of the HPV testing prior and after the therapy. Patients were randomized into two groups: the first group was HPV positive woman treated with other than recommended therapy (n=20), (control group); the second group was pharmacologically treated with intravaginal administration of an interferon and aloe vera-propolis in recommended scheme (n=35) with treatment of the possible fungal or bacterial genital infection prior to the specific therapy. The

  11. 靛胭脂联合美蓝染色在早期胃癌及癌前病变诊断中的价值%Value of Combined Staining with Indigo Carmine and Methylene Blue in Diagnosis of Early Gastric Cancer and Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    朱国福; 朱学鹰; 祝三秀

    2015-01-01

    目的:探讨靛胭脂联合美蓝染色在诊断早期胃癌及癌前病变中的临床应用价值。方法将300例内镜下胃黏膜异常表现患者随机分为2组:染色组150例内镜下先喷洒0.2%靛胭脂染色,发现可疑病灶后,再喷洒0.4%美蓝染色,然后在染色异常部位给予活检并送病理组织学检查;对照组150例不作染色,按肉眼判断,常规活检。结果染色组150例患者中胃黏膜有肠上皮化生38例、轻-中度不典型增生50例、重度不典型增生9例、早期胃癌8例,对照组150例患者中肠上皮化生29例、轻-中度不典型增生19例、重度不典型增生2 例。染色组早期胃癌及癌前病变总检出率显著高于对照组(70.0%比33.3%,P <0.005)。结论靛胭脂联合美蓝染色能够指导内镜下上消化道病变的活检,能有效提高早期胃癌和癌前病变的检出率。%Objective To investigate the clinical value of combined staining with indigo carmine and methylene blue in the diagnosis of early gastric cancer and precancerous lesions.Methods A total of 300 patients with endoscopic abnormalities of gastric mucosa were randomly divided into two groups,with 150 patients in each group.In staining group,0.4% methylene blue was sprayed after suspicious lesions were observed by endoscopic staining with 0.2% indigo carmine,and then biopsy at abnormal staining sites was performed for histopathologic examination.In control group,patients only received naked eye examination and routine biopsy.Results Among the 150 patients in staining group,38 had gastric intestinal metaplasia,50 had mild to moderate atypical hyperplasia,9 had severe atypical hyperplasia,and 8 had early gastric cancer.Among the 150 pa-tients in control group,29 had gastric intestinal metaplasia,19 had mild to moderate atypical hy-perplasia,and 2 had severe atypical hyperplasia.The detection rate of early gastric cancer and pre-cancerous lesions in staining

  12. Clinical Value of Endoscopic Multi-band Mucosectomy in Treatment of Early Esophageal Cancer and Precancerous Lesions%内镜下多环黏膜切除术在治疗早期食管癌及癌前病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    刘晓波; 高子夜; 金曙; 李胜保; 童强; 王强

    2014-01-01

    Objective To investigate the feasibility,safety and efficacy of endoscopic multi-band mucosectomy (EMBM) in the diagnosis and treatment of early esophageal cancer and precancerous lesions.Methods A total of 33 patients with early esophageal cancer and precancerous lesions were confirmed by endoscopy,ultrasound endoscopy and pathology.The lesion extent was confirmed and observed by using Lugol staining before the EMBM was preformed under general anesthesia.Meanwhile,the specimens from the patients were sent for pathological examination.After the operation,all patients were regularly rechecked by gastroscope,and clinical effect in patients were also evaluated.Results Thirty-five lesions in 33 patients were successfully removed,the rate of complete resection was 100%.The pathological characteristic in 33 patients showed 2 cases of slightly atypical hyperplasia,8 cases of moderately atypical hyperplasia,16 cases of severe atypical hyperplasia,and 7 cases of early esophageal squamous cell carcinoma.The coincidence rate of pathological examination was 81.8% (27/33).Seven complications in 33 patients were observed,in which 2 cases underwent endoscopic hemostasis.All cases did not show the complications such as perforation,obstruction and infection.Wound exudation were not observed 2 weeks after surgery,the wound were completely healed and scar formation have been observed 4 weeks after the operation,and recurrence was not observed in all cases after 6 to 8 months follow-up.Conclusion EMBM is worthy of popularization for treating early esophageal cancer and precancerous lesions because of its advantages of easy operation,high safety,exact curative effect and less complications.%目的:探讨内镜下多环黏膜切除术(endoscopic multi-band mucosectomy,EMBM)在早期食管癌及癌前病变治疗中的可行性、安全性及有效性.方法:33例经普通胃镜、超声胃镜及病理学检查明确诊断为早期食管癌及癌前病变患者,术前行Lugol碘

  13. 宫颈环形电切术治疗宫颈病变278例效果观察%Cervical loop electrosurgical excision procedure in the treatment of cervical lesions:report of 278 cases

    Institute of Scientific and Technical Information of China (English)

    欧世贤

    2014-01-01

    目的:观察宫颈环形电切术( LEEP)治疗宫颈病变的临床疗效。方法选择宫颈病变患者278例行阴道镜下活检后,采用LEEP治疗,观察手术时间、出血量和治疗效果。结果278例患者手术均成功。手术时间5~20 min,平均7.5 min;术中出血量为10~60 ml,平均12.8 ml。治愈257例,好转13例,无效8例,总有效率为97.1%。结论 LEEP治疗宫颈病变安全有效,可提高治愈率。%Objective To investigate the clinical effect of cervical loop electrosurgical excision procedure in the treatment of cervical lesions .Methods Two hundred and seventy-eight patients with various cervical lesions un-derwent colposcopic biopsy , then were treated by cervical loop electrosurgical excision procedure .The operation time and bleeding volume were observed , the therapeutic effect and safety were analyzed retrospectively .Results In 278 patients treated by cervical loop electrosurgical excision procedure all operations were successful .The operation time ranged from 5 to 20(average 7.5) min;intraoperative blood loss was 10 to 60(average 12.8) ml;257 patients were cured, 13 patients improved , the total effective rate was 97.1%.Conclusion Cervical loop electrosurgical excision procedure treatment of cervical lesions is safe and effective , and can improve the cure rate .

  14. Non-carious cervical lesions associated with multiple gingival recessions in the maxillary arch. A restorative-periodontal effort for esthetic success. A 12-month case report.

    Science.gov (United States)

    Allegri, Mario Alessio; Landi, Luca; Zucchelli, Giovanni

    2010-01-01

    Restoration of non-carious cervical lesions (NCCLs) represents a major challenge for resin materials due to the different adhesive properties of the tooth structure, the biomechanical aspects of the cervical area, and the difficulties in the access and isolation of the operative field. Furthermore, NCCLs should be approached with a complete understanding of the role played by the marginal periodontal tissue. Whenever a cervical lesion is associated with a gingival recession, the interplay between restorative dentistry and periodontology is decisive for full esthetic and long-term success. A case report is presented dealing with the treatment of NCCLs associated with multiple gingival recessions using a combined restorative and periodontal treatment with a 12-month follow-up.

  15. Clinical Application of Colposcopy Cervical Biopsy and Liquid-based Cytology Test in the Screening of Cervical Lesions%阴道镜宫颈活检与 TCT 联合检测在宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    冷海娜; 孙名芳

    2013-01-01

    Objective To explore the clinical application value of colposcopy cervical biopsy and liquid -based cytology test in the screening of cervical lesions .Methods Four hundred and eighty-three patients who udergone the TCT examination from January to December in gynecological clinic were chosen for pathological colposcopy cervical biopsy To select 483 gynecological clinic patients who had the TCT examination and were inspected to be abnormal in Care Hospital from January to December ,2012 ,have pathological colposcopy cervical biopsy for them and retrospective a-nalysis fwas carried out .or their entire clinical datum .Among which the TBS diagnostic system wasis used in the TCT in-spection report ,which contributed to the diagnosis ,as well as combined with the pathological examination report to make the diagnosis.Results Among the patients with the TCT result being ASC-US351,CINⅠ~CINⅢ223 patients(63. 53%) were detected with the pathological colposcopy cervical biopsy;CINⅠ~CINⅢ75 patients(91.46%) were de-tected with the pathological colposcopy cervical biopsy;CINⅠ~CIMⅢ41 patients were detected with the pathological colposcopy cervical biopsy and among the 6 SCC patients ,SCC 6 patients were detected with the colposcopy cervical bi-opsy .Conclusion The diagnose accordance rate of TCT examination and cervical biopsy diagnosis is high ;the diagnose accordance rate of cervical cancer with CIN of higher level is high .Precancerous lesions of uterine cervix can be found at an earlier age with the adoption of colposcopic cervical biopsy combined with TCT examination and stopped or slowed down ,which is the safe ,reliable and accurate examination method for the screening of cervical cancer .%目的:探讨阴道镜宫颈活检与宫颈薄层液基细胞学( TCT )在宫颈病变筛查中的临床应用价值。方法选取2012年1月~12月在妇科门诊行宫颈薄层液基细胞学( TCT)检查异常的483例患者,并同时行阴道镜宫颈活检

  16. ANÁLISIS COMPUTACIONAL DE LESIONES CERVICALES NO CARIOSAS EN UN PREMOLAR SUPERIOR COMPUTER ANALYSIS OF NON-CAVITY CERVICAL LESIONS IN AN UPPER PREMOLAR

    Directory of Open Access Journals (Sweden)

    Patricio Cendoya

    2007-08-01

    Full Text Available Se presenta un modelo tridimensional de elementos finitos para investigar el efecto producido por las fuerzas de oclusión en la distribución y magnitud del campo tensional sobre un premolar superior. De esta forma, se busca definir cuál es la situación de carga de oclusión más crítica desde el punto de vista tensional sobre el esmalte dental en la zona cervical que pudiese dar origen a una lesión del tipo no cariosa. Utilizando elementos finitos hexaédricos lineales y procesando el modelo con el programa SAMCEF se realiza un análisis numérico estático lineal considerando que el premolar es isótropo y homogéneo. Se definen siete estados de carga asociados a una fuerza de oclusión de 170 N para las cuales se investiga la distribución y magnitud de los campos tensionales sobre el premolar. Los resultados numéricos permiten concluir que las tensiones máximas sobre el premolar tienden a concentrarse en la zona cervical alcanzando sus valores máximos cuando la carga de 170 N actúa de forma inclinada a 45º con respecto a la vertical. La relevancia de la presente investigación radica en el hecho que numéricamente se demuestra que la distribución y zona en donde los campos tensionales tienden a concentrase y alcanzar sus valores máximos son coincidentes con las zonas del diente en donde clínicamente se observan lesiones no cariosas.A three-dimensional model of finite elements in order to investigate the effect produced by occlusion forces on both distribution and magnitude of the tensional field on an upper premolar is hereby presented. Thus, defining from a tensional point of view, which is the situation of the most critical occlusion loading on dental enamel capable to produce a non-cavity lesion is intended. By using finite linear hexahedral elements and processing the models through SAMCEF software, a static linear numerical analysis is performed, considering that premolar is isotropic and homogeneous. Seven load states

  17. Feasibility of fine-needle aspiration biopsy and its applications in superficial cervical lesion biopsies.

    Science.gov (United States)

    Xu, Dong; Xu, Hai-Miao; Li, Ming-Kui; Chen, Li-Yu; Wang, Li-Jing

    2014-01-01

    The aim of the study was to investigate the feasibility and value of clinical application of fine-needle aspiration histological biopsy via ultrasound-guided thyroid nodule and enlarged cervical lymph node fine-needle aspiration histological biopsy. Fine-needle aspiration cytological and histological biopsies and surgical treatments were performed on 982 patients with thyroid nodule and 1435 patients with enlarged cervical lymph nodes. A comparative study of the histological and cytological examination results and post-surgical etiology results was subsequently conducted. Among the 982 thyroid nodule patients, the acquisition rates were 89.8% (882/982) for fine-needle aspiration histological biopsy and 96.2% (945/982) for cytological biopsy, while among the 1435 patients with enlarged cervical lymph nodes, the acquisition rate for fine-needle aspiration cytological biopsy was slightly higher than that for histological biopsy, with values of 95.7% (1374/1435) and 91.4% (1312/1435), respectively. For the thyroid nodule patients, when the acquired histological and cytological biopsy results were compared with the post-surgical etiology results, the sensitivity, specificity, and accuracy of the histological results were 98.5%, 100%, and 98.9%, respectively, whereas those of the cytological results were 86.8%, 82.9%, and 85.6%, respectively; the differences between the 2 biopsy methods were statistically significant (P < 0.05). For the patients with enlarged cervical lymph nodes, when the acquired histological and cytological biopsy results were compared with the post-surgical etiology results, the sensitivity, specificity, and accuracy of the histological results were 96.3%, 99.8%, and 97.6%, respectively, whereas the those of the cytological results were 76.8%, 92.1%, and 82.2%, respectively; again, the differences between the 2 methods were statistically significant (P < 0.05). In conclusion, Fine-needle aspiration histological biopsy is a reliable and highly

  18. Restoration of non-carious cervical lesions with ceramic inlays: A possible model for clinical testing of adhesive cements

    Directory of Open Access Journals (Sweden)

    Michal Staninec

    2012-01-01

    Full Text Available Introduction: There are many luting cements coming to market which claim to be adhesive, but there is no clinical protocol currently for testing these claims. There is a standardized protocol for testing direct restorations bonded to dentin and it is used extensively. Case Report: We describe a clinical procedure for restoring a non-carious cervical lesion (NCCL with a ceramic inlay using Computer-Aided Design and Computer-Aided Manufacturing (CAD-CAM technology and an adhesive resin cement.The procedure was straightforward and the result was good at one month. Discussion: NCCL′s can be restored with CAD-CAM technology in one appointment. This technique can be used to clinically test adhesion of luting cements to dentin, similarly to the current standard for direct restorations.

  19. The Distribution and Infection of HPV in Cervical Biopsy Lesions among 450 Women%450例宫颈活检组织中人乳头瘤病毒的分布特征

    Institute of Scientific and Technical Information of China (English)

    王翔; 钱福初; 顾栋桦; 秦基取; 陈瑜; 李雄峰

    2011-01-01

    [Objective] To determine the distribution and infection of HPV in cervical cancer and precancerous lesions. [Methods]Four hundred patients with abnormal cytology were examined by colposcopy. HPV diagnosis was done by polymerase chain reaction using MY09/MY11 primers, for genotyping blot hybridization was used. The pathological results confirmed standards. [Result]A total of 294 women were HPV-DNA positive. HPV-16 was found in 36.7% of all women with positive HPV-DNA,HPV58 in 22. 1%, HPV-18 in 18.7% and HPV-52 in 16. 0%. The infection of HPV in lesions with cervicitis, CIN Ⅰ , CIN Ⅱ and CIN Ⅲ were 55.6 %, 69 %, 82.7 % and 88.9%, respectively. HPV-16,-58,-18 and -52 were found in different cervical lesions. [Conclusions] The finding of HPV-58 and -52 expects HPV-16 and -18 among Huzhou women with CIN Ⅱ and CIN Ⅲ , and has important implications for the development of type-common HPV vaccines.%[目的]了解阴道镜检查患者的宫颈活检组织中HPV的感染率和亚型分布.[方法]对宫颈脱落细胞学异常的患者450例进行阴道镜下定位活检.活检组织同时行HPV基因分型和病理诊断,HPV基因分型采用斑点杂交方法进行.[结果]在450例宫颈活检患者中,其HPV感染率为65.3%.在HPV-DNA阳性患者中,检出率最高的HPV-16占36.7%,依次HPV-58为22.1%,HPV-18为18.7%,HPV-52为16.0%.在宫颈炎、CIN Ⅰ、CIN 、Ⅱ、D,CIN Ⅲ中HPV-DNA阳性率分别为55.6%、69%、82.7%和88.9%.在宫颈炎、CIN Ⅰ、CIN Ⅱ、CIN Ⅲ组织中均检测到HPV-16、-58、-18和-52.[结论]本研究中的宫颈活检组织中不仅检测到HPV-16和-18,还检测到HPV-58和-52感染,后者的发现为开发宫颈癌多价复合型疫苗提供了事实依据.

  20. 莪术油对大鼠乳腺癌癌前病变组织中VEGF mRNA表达的影响%Zedoary turmeric oil effect on rats with precancerous lesions of breast carcinoma in VEGF mRNA expression

    Institute of Scientific and Technical Information of China (English)

    宋爱莉; 许振国

    2012-01-01

    Objective: Through observing the influence of zedoary turmeric oil on rats with precancerous lesions of breast carcinoma tissue in vascular endothelial growth factor ( VEGF ) and its mRNA expression, discuss the mechanism of Zedoary turmeric oil treating preeancerous lesions of breast cancer- Method: 275 SD rats were randomly divided into control group, model group, the TAM group, injection group and zedoary turmeric oil, in small, high dose group,then use DMBA induced the model of precancerous lesions of breast cancer. After intervention treatment for 4 weeks, the animals were sacrificed in 4 batches after 8-14 weeks, using insitu method for the determination of specimens of VEGF mRNA expression. Result; Each lest group of different types of breast tissue in VEGF mRNA expression positive rate and positive cells positive rate increased, A typical hyperplasia of breast tissue,The VEGF mRNA expression positive rate in the intervention group were significantly lower than those in model group,and the intervention group VEGF mRNA positive cells positive rate were significantly lower than those in model group, Zedoary turmeric oil group is lower than injection and TAM group ,In the atypical hyperplasia specimens of VEGF mKNA expression, VEGF mRNA positive cells positive rate of Zedoary turmeric oil the in the large dose group was significantly lower than small dose group. Conclusion: Zedoary turmeric oil can effectively reduce DMBA induced rat mammary carcinoma precancerous lesion tissue VEGFmRNA expression intensity and Inhibit angiogenesis, so May he the effective mechanism of blocking in breast cancer.%目的:通过观察莪术油对大鼠乳腺癌癌前病变组织中血管内皮生长因子(VEGF)及其mRNA表达的影响,探讨莪术油治疗乳腺癌前病变的机理.方法:275只SD大鼠随机分为空白对照组、疾病模型组、三苯氧胺组、康莱特组及莪术油小、中、大剂量组.采用DMBA诱导乳腺癌癌前病变造模,干预治疗4

  1. 透明帽法内镜黏膜切除术与多环黏膜套扎切除术治疗早期食管癌及癌前病变的比较研究%Comparative study of endoscopic musoucal resection with transparent cap and endoscopic multiband mucosectomy for early esophageal cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    张月明; 张蕾; 于桂香; 鞠凤环; 荀华英; 朱娜; 王贵齐; 贺舜; 薛丽燕; 吕宁; 程贵余; 秦秀敏; 窦利州; 赖少清; 倪晓光

    2012-01-01

    目的 比较透明帽法内镜下黏膜切除术(EMR-Cap)与多环黏膜套扎切除术(MBM)治疗早期食管癌及癌前病变的疗效和安全性.方法 回顾性分析2008年12月至2009年12月间在中国医学科学院肿瘤医院内镜科接受EMR-Cap治疗的30例(EMR-Cap组)及2010年1月至2011年1月间接受MBM治疗的32例(MBM组)早期食管癌及癌前病变患者的临床资料,比较两种技术的疗效、安全性及费用.结果 EMR-Cap组平均病变切除时间和治疗总时间分别为26 min和43 min,明显长于MBM组的10 min和32 min(P=0.036,P=0.038).切除病变总厚度和黏膜下切除深度两组差异无统计学意义(均P>0.05).EMR-Cap组平均治疗费用为(5466±354)元,明显高于MBM组的(4014±368)元(P=0.008).EMR-Cap组出现术后狭窄1例,MBM组出现术中穿孔1例.术后随访17~42个月,无一例局部复发,EMR-Cap组出现1例淋巴结转移.结论 EMR-Cap和MBM均是治疗早期食管癌和癌前病变微创、安全和有效的手段.在保证相同治疗效果的前提下,与EMR-Cap相比,MBM具有操作简单、治疗时间短、治疗成本低的优点,适宜广泛推广和开展.%Objective To evaluate the efficacy and safety of endoscopic musoucal resection with transparent cap (EMR-Cap) and endoscopic multi-band mucosectomy (MBM) in the treatment of early esophageal cancer and precancerous lesion.Methods A retrospective study was performed to review 30 EMR-Cap cases from December 2008 to December 2009 and 32 MBM cases from January 2010 to January 2011 of early esophageal cancer and precancerous lesions.The differences between these two techniques in efficacy,safety,and cost were compared.Results In EMR-Cap group,the median resection time was 26 (10-56) min and median procedure time was 43 (22-81) min,significantly longer than those in MBM group [ 10 (7-18) min and 32 (28-45) min,P=0.036 and 0.038,respectively].There were no significant differences between the two groups in total thickness and depth

  2. Clinical impact of de-regulated Notch-1 and Notch-3 in the development and progression of HPV-associated different histological subtypes of precancerous and cancerous lesions of human uterine cervix.

    Directory of Open Access Journals (Sweden)

    Richa Tripathi

    Full Text Available BACKGROUND: Cervical cancer is the leading cause of cancer related deaths among women in India. Limited reports are available for Notch-1 and Notch-3 protein in cervical carcinoma, which play crucial role in cell proliferation, differentiation, and apoptosis. METHODS: This study was designed to evaluate the role of Notch-1 and Notch-3 with context to HPV infection in cervical carcinoma. A total of 168 tissue biopsy samples comprising of tumor specimens (n = 98, precancer (n = 30 and non-neoplastic cervical tissues (n = 40 were screened for HPV infection by PCR and expression of Notch-1 and Notch-3 protein by Immunohistochemistry and Immunoblotting. RESULTS: 80% (24/30 were found to be positive for HPV in precancer and 86.7% (85/98 in cancer patients. Notch-1 expression of precancer and cancer cases was found to be significantly down-regulated with severity of disease in nuclear (3.43±0.29; 2.04±0.19, p = 0.0001, p = 0.0001 and cytoplasm (3.07±0.29; 2.29±0.17, p = 0.0001, p = 0.0001 obtained from different stages as compared to normal cervix tissue (5.40±0.19, 4.97±0.15; p<0.001; p<0.001. However, Notch-3 expression of above cases was significantly up-regulated with severity of disease and showed intense nuclear (4.17±0.39; 4.74±0.18, p = 0.0001, p = 0.0001 and cytoplasm (3.67±0.36; 4.48±0.18, p = 0.0001, p = 0.0001 of different stages as compared to normal cervix tissue (0.95±0.20, 0.70±0.20; p<0.001; p<0.001 respectively. CONCLUSIONS: These findings suggest that Notch-1 and Notch-3 may play an important role with synergistic effect of HPV in regulating development and proliferation of cervical cancer through the deregulation of Notch signalling. This study also shows the clinical utility of both proteins which may be used as predictable biomarkers in diagnosing different histological sub-types of HPV associated cervical cancer. Nevertheless, abnormal activation of this pathway may provide

  3. Study on the clinical treatment of low - grade squamous intraepithelial lesion diagnosed by cytological test%宫颈细胞学诊断低度上皮内瘤变的临床处理探讨

    Institute of Scientific and Technical Information of China (English)

    张娟; 蔡林儿; 严杏

    2012-01-01

    目的:探讨宫颈细胞学诊断低度上皮内瘤变的意义及临床处理方法.方法:对2010年5月~2011年11月在广东省中医院就诊并经TCT检查为LSIL的195例女性病例进一步行HPV-DNA-PCR检测及阴道镜检查,观察其最终病理结果的构成及在不同年龄阶段的分布差异.结果:在195例LSIL患者中,高危HPV阳性144例(73.8%),病理诊断为癌前病变及癌者共87例(60.4%);高危HPV阴性51例(26.2%),病理诊断为癌前病变及癌者共8例(15.7%).在35岁以下的93例中,病理诊断为癌前病变及癌者共43例(46.2%);在35~45岁的69例中,病理诊断为癌前病变及癌者共41例(69.9%);45岁以上33例中,病理诊断为癌前病变及癌者共11例(33.3%).结论:在TCT诊断为LSIL的患者中,高危HPV阳性与高危HPV阴性患者的宫颈病变检出率差异有统计学意义,不同年龄段之间宫颈病变检出率差异有统计学意义,临床处理方法及随访计划的制定与年龄及HPV感染情况有关.%Objective: To explore the significance of cervical cytological test for diagnosis of low - grade squamous intraepithelial lesion (LSIL) and the clinical treatment. Methods: A total of 195 female cases who were diagnosed as low grade squamous intraepithelial lesion (LSIL) by TCT in the hospital from May 2010 to November 2011 further underwent HPV - DNA PCR and colposcopy, the proportion of finally pathological results and the difference of different age distributions were observed. Results: Among 195 patients with LSIL, 144 patients (73. 8% ) were found with positive high risk HPV, 87 patients (60. 4% ) were diagnosed as cervical precancerous lesion and cervical cancer by pathological diagnosis; 51 patients (26. 2% ) were found with negative high risk HPV, 8 patients (15. 7% ) were diagnosed as cervical precancerous lesion and cervical cancer by pathological diagnosis. Among 93 patients under 35 years old, 43 patients (46.2% ) were diagnosed as cervical precancerous

  4. Effect Observation on HPV, TCT and Vaginal Examination in Cervical Le-sions and Cervical Screening%宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果探讨

    Institute of Scientific and Technical Information of China (English)

    金伟蓉; 孔彩霞

    2015-01-01

    目的:探讨宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果。方法整群选取2013年5月—2015年5月于该院接受TCT及HPV检查的妇女1280例。对这些患者再进行阴道镜检查以及宫颈组织活检,然后将检验的结果与病理结果进行统计学的分析比对。结果 TCT检查的阳性检出率为9.61%(123/1280);HPV检查的阳性检出率为12.03%(154/1280)。结论临床上对宫颈病变结合筛查可联合CTC、HPV以及阴道镜检查,这样可提高宫颈病变的检出率,减少漏诊,对临床的及时治疗提供极大的帮助。%Objective To observe the effect of HPV, TCT and vaginal examination in cervical lesions and cervical screen-ing. Methods 1280 cases of women who received TCT and HPV examination in our hospital from May 2013 to May 2015 were selected and were given vaginal examination and cervical biopsy, the test results and the pathological results were compared and analyzed statistically. Results The positive detection rate of TCT was 9.61% (123/1280)and 12.03%(154/1280)of HPV. Conclusion HPV, TCT and vaginal examination in treatment of cervical lesions and cervical screening can improve the detection rate of cervical lesions, reduce missed diagnosis and offer great help to timely treatment clinically.

  5. Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test

    Directory of Open Access Journals (Sweden)

    Caroline Tanski Bueno

    2014-09-01

    Full Text Available The aim of this study was to evaluate the association between the frequency of micronuclei (MN and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p < 0.001. The mean MN frequencies were 0.95 ± 1.12 (mean ± SD in the control group (n = 223, 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US (n = 50, 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN I (n = 52, 5.97 ± 1.83 in CIN II (n = 30, 7.29 ± 1.55 in CIN III (n = 17 and 8.64 ± 1.55 in invasive cancer (n = 25. These findings suggest that MN monitoring should be included as an additional criterion for the early detection of cytogenetic damage in routine examinations. This monitoring should be done in the same smear as used for cytopathological examination. More specific and systematic studies are necessary to confirm this proposal.

  6. Association between cervical lesion grade and micronucleus frequency in the Papanicolaou test.

    Science.gov (United States)

    Bueno, Caroline Tanski; Dornelles da Silva, Cláudia Maria; Barcellos, Regina Bones; da Silva, Juliana; Dos Santos, Carla Rossana; Menezes, João Evangelista Sampaio; Menezes, Honório Sampaio; Rossetti, Maria Lucia Rosa

    2014-09-01

    The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p < 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II (n = 30), 7.29 ± 1.55 in CIN III (n = 17) and 8.64 ± 1.55 in invasive cancer (n = 25). These findings suggest that MN monitoring should be included as an additional criterion for the early detection of cytogenetic damage in routine examinations. This monitoring should be done in the same smear as used for cytopathological examination. More specific and systematic studies are necessary to confirm this proposal.

  7. 胃舒胶囊对慢性萎缩性胃炎癌前病变增殖细胞核抗原及AgNOR的影响%Effect of Weishu Capsule on PCNA,AgNOR of Precancerous Lesions of Chronic Atrophic Gastritis

    Institute of Scientific and Technical Information of China (English)

    陆为民; 单兆伟; 沈洪; 吴静; 朱云华; 朱长乐

    2001-01-01

    Objective:To explore the therapeutic effect and mechanism of Weishu capsule (WSC) in treating precancerous lesions of chronic atrophic gastritis(CAG).Methods:192 cases of precancerous lesions of CAG were distributed to two groups randomly,the treated group(128 patients) was treated with WSC by taken orally and the control group (64 patients) was treated with Weining granule(WNG) orally.The treatment of course of both groups were 6 months.The clinical,gastroscopic and pathological effects and the changes in numbers of AgNOR granule and scores of PCNA expression were observed before and after treatment.Results:The total effective rate was 91.4% and the effective rate of gastroscope,atrophy,intestinal metaplasia,dysplasia in the treated group were 70.3%,64.1%,66.4%,67.9% respectively, and the respective score were lowered significantly after treatment.Immunohistochemical test showed that WSC could reduce the numbers of AgNOR granule and inhibit the expression of PCNA significantly,compared with the control group,the difference between the two groups was significant(P<0.01,<0.05).Conclusion:WSC could significantly improve the clinical,gastroscopic and pathological changes in the precancerous lesions of CAG.The mechanism might be related to inhibit and correct the abnormal proliferation and differentiation of cells.%目的:探讨胃舒胶囊对萎缩性胃炎癌前病变的治疗作用及机制。方法:192例萎缩性胃炎癌前病变患者随机分为两组,治疗组(128例)给予胃舒胶囊口服,对照组(64例)给予胃宁冲剂冲服,疗程均为6个月。观察两组患者临床、内镜、病理疗效及治疗前后AgNOR颗粒数、增殖细胞核抗原(PCNA)表达积分值的变化。结果:治疗组临床总有效率为91.4%,胃镜有效率70.3%,对萎缩、肠上皮化生、异型增生的有效率分别为64.1%、66.4%、67.9%,并能降低其积分值。治疗组治疗前后免疫组化结果显示,胃舒胶囊也能显

  8. Gene reversal mechanism of Weifuchun combined with colloidal bismuth pectin on patients with precancerous lesion of gastric carcinoma%胃复春联合胶体果胶铋对胃癌前病变基因的逆转性研究

    Institute of Scientific and Technical Information of China (English)

    郑波; 王建嶂; 裴继华; 李国刚; 薛战雄

    2016-01-01

    目的:观察胃复春联合胶体果胶铋胶囊对胃癌前病变患者Notch1、β-链蛋白(β-catenin )、人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)的影响。方法115例胃癌前病变患者随机分为试验组60例,对照组55例。试验组口服胃复春1.44 g,胶体果胶铋150 mg,每天3次;对照组口服奥美拉唑20 mg,每天2次,口服硫糖铝0.25 g,每天3次,2组均连续治疗4周。观察2组患者治疗前后Notch1、β-catenin及PTEN的表达。结果治疗后,试验组的总有效率(68.3%)显著高于对照组(43.6%, P<0.01)。治疗后,试验组Notch1、β-catenin阳性率与治疗前相比均有所下降,且试验组比对照组下降更显著( P<0.01)。治疗后试验组PTEN阳性表达显著提升,且优于对照组( P<0.05)。结论胃复春联合胶体果胶铋胶囊不仅能够降低胃癌前病变中Notch1、β-catenin表达,提升PTEN表达,同时对提高治疗效果和治疗安全性也具有显著的效果。%Objective To discuss the effect of Weifuchun combined with colloidal bismuth pectin capsules on Notch 1, β-catenin and phosphatase and tensin homolog deleted on chromosome ten ( PTEN) in patients with precancerous lesion of gastric carcinoma.Methods A total of 115 patients with precancerous lesion of gastric carcinoma were randomly divided into experiment group ( n =60 ) and control group (n=55).Patients in experiment group were given Weifuchun 1.44 g, colloidal bismuth pectin capsules 150 mg, 3 times a day, while patients in control group given omeprazole 20 mg, 2 times a day, and sucralfate 0.25 g, 3 times a day, all patients treated for 4 weeks.The expressions of Notch1, β-catenin and PTEN of the two groups were observed.Results After treatment , the total effective rate of experiment group (68.3%) was significantly higher than that of control group (43.6%, P<0.01 ).After treatment , the positive rates of Notch 1

  9. The prevalence and morphological types of non-carious cervical lesions (NCCL) in a contemporary sample of people.

    Science.gov (United States)

    Igarashi, Yuriko; Yoshida, Satoru; Kanazawa, Eisaku

    2017-03-08

    Non-carious cervical lesions (NCCLs) were examined in 6541 extracted human teeth and classified based on the morphology of the lesions. As a result, NCCLs were found on 38.7% of teeth (41.6% on maxillary teeth and 36.0% on mandibular teeth), and were most frequent on canines and first premolars. According to the new method of classification, the morphology of NCCLs was classified both by the surface contour (SC) and by the cross-sectional contour (CC). Three types of NCCLs appeared to be dominant. The causes of these NCCLs were discussed based on their morphologies, positions where these NCCLs were frequently found, and the results of previous studies. NCCLs with a horizontal oval SC and a round CC (Type I), which were frequent on the labial surfaces of maxillary canines and buccal surfaces of maxillary first premolars, may be associated with wear by friction and chemical degradation. NCCLs with a vertical oval SC and a round CC (Type II), which were frequent on the lingual surfaces of mandibular incisors and canines, might be mainly related to chemical degradation. NCCLs with a horizontal oval SC and a wedge shape CC (Type III), which were extensively found on the buccal surfaces of maxillary premolars, had formed most probably due to wear by friction and microstructural loss by stress. This new method can classify the morphology of NCCLs more precisely and deduce the mechanisms of the formation of NCCLs more clearly than former methods.

  10. Chemical Interaction Analysis of an Adhesive Containing 10-Methacryloyloxydecyl Dihydrogen Phosphate (10-MDP) With the Dentin in Noncarious Cervical Lesions.

    Science.gov (United States)

    Oliveira, Bmb; Ulbaldini, Alm; Sato, F; Baesso, M L; Bento, A C; Andrade, Lhc; Lima, S M; Pascotto, R C

    2017-02-03

    The purpose of this study was to evaluate the chemical bonds of a self-etch 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) adhesive to natural noncarious cervical lesions (NCCLs) and compare them with those occurring in sclerotic dentin in artificially prepared defects (APDs). Four human teeth with natural NCCLs on the buccal surface were selected. Artificial defects matching the natural lesions were prepared on the lingual surface of the same teeth serving as control. Micro-Raman (MR) spectroscopy was used to quantify mineral content in natural NCCLs and in APDs. Fourier transform infrared-photoacoustic spectroscopy (FTIR-PAS) readouts were taken before and after adhesive application to analyze the protein matrix/mineral (M:M) ratio and chemical interactions between 10-MDP adhesive and dentin. The MR and FTIR-PAS spectra collected from natural NCCLs demonstrated a larger area of the band (961 cm(-1), PO4) and lower M:M ratio, respectively, characterizing a hypermineralized dentin, compared with APDs. FTIR-PAS demonstrated emergence of a peak (1179 cm(-1), P=O) in spectra after adhesive treatment, demonstrating a more intense chemical interaction in natural NCCLs. The results demonstrated that chemical bonding of 10-MDP adhesive to natural NCCLs is more intense, due to the hypermineralized surface, and suggest that it is unnecessary to remove the hypermineralized layer with burs, as this may decrease the chemical bonding potential of 10-MDP.

  11. Diagnosis Value of Liquid-based Cytology for the Cervical Precancerous Lesion and Cervical Cancer%液基薄层细胞学在宫颈癌前病变和宫颈癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    苏学治; 胡大学; 刘翔

    2009-01-01

    目的:探讨液基薄层细胞学检测在宫颈癌前疾病诊断中的临床价值.方法:采用第三代液基细胞学制片系统(LPT)制片,按TBS(The bethesda system)系统诊断为不能明确诊断意义的非典型鳞状细胞(ASCUS)以上的768例,其中低度病变(LSIL)以上184例;对有病理组织学诊断对照的139例进行了对比分析.结果:139例中细胞学诊断宫颈癌1例,宫颈高度病变(HSIL)13例,宫颈低度病变(LSIL)120例,非典型细胞不能除外高度病变(ASC-H)5例;与组织学结果对照,阳性符合率分别为癌100%,HSIL 84.6%,LSIL 70%.5例ASC-H中2例宫颈癌,l例宫颈上皮内瘤变(CIN)2级,1例CIN 1级~2级,1例湿疣.结论:液基细胞学在诊断宫颈癌前病变和官颈癌方面,与组织学诊断结果有较高的符合率,因而具有较高的临床应用价值.

  12. Diagnostic aids for detection of oral precancerous conditions

    Institute of Scientific and Technical Information of China (English)

    Diana V Messadi

    2013-01-01

    Oral cancer has a tendency to be detected at late stage which is detrimental to the patients because of its high mortality and morbidity rates. Early detection of oral cancer is therefore important to reduce the burden of this devastating disease. In this review article, the most common oral precancerous lesions are discussed and the importance of early diagnosis is emphasized. In addition, the most common non-invasive oral cancer devices that can aid the general practitioners in early diagnosis are also discussed.

  13. Analysis on the diagnosis and treatment of 198 cases with cervical lesions treated by LEEP%198例宫颈疾病的LEEP刀诊治分析

    Institute of Scientific and Technical Information of China (English)

    马玉华

    2011-01-01

    Objective: To explore the clinical application value of loop electrosurgical excision procedure (LEEP) in diagnosis and treatment of various cervical lesions.Methods: 198 cases with chronic cervical inflammation, cervical hyperplasia and cervical neoplasma received LEEP and they were followed up.Results: 198 cases were treated by LEEP and followed up for 3 months, 191 cases were cured successfully for the first time, accounting for 96.5%, 7 cases were effective, the total effective rate was 100.0%, 5 cases were diagnosed as cervical intraepithelial neoplasia (CIN).Conclusion: The clinical efficacy of LEEP is significant in treatment of various cervical lesions,the operation time is short and the operation is simple, LEEP can provide intact pathological samples and reduce the misdiagnosis rate and missed diagnosis rate of cervical cancer.%目的:探讨LEEP刀对各类宫颈疾病诊断及治疗的临床应用价值.方法:对198例宫颈慢性炎症、增生及赘生物患者进行LEEP刀宫颈环切或锥切并跟踪观察.结果:198例各类宫颈良性疾病患者经LEEP刀治疗,连续观察3个月,一次性成功治愈191例(96.5%),显效7例,总有效率为100.0%,其中宫颈上皮内瘤变(CIN)5例.结论:LEEP刀治疗各类宫颈疾病疗效显著,手术时间短,操作简单易行,且可提供完整的病理标本,降低了宫颈癌的误诊率和漏诊率.

  14. Human papillomavirus 16 non-European variants are preferentially associated with high-grade cervical lesions.

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    Luciana Bueno Freitas

    Full Text Available HPV16 accounts for 50-70% of cervical cancer cases worldwide. Characterization of HPV16 variants previously indicated that they differ in risks for viral persistence, progression to cervical precancer and malignant cancer. The aim of this study was to examine the association of severity of disease with HPV16 variants identified in specimens (n = 281 obtained from a Cervical Pathology and Colposcopy outpatient clinic in the University Hospital of Espírito Santo State, Southeastern Brazil, from April 2010 to November 2011. All cytologic and histologic diagnoses were determined prior to definitive treatment. The DNA was isolated using QIAamp DNA Mini Kit and HPV was detected by amplification with PGMY09/11 primers and positive samples were genotyped by RFLP analyses and reverse line blot. The genomes of the HPV16 positive samples were sequenced, from which variant lineages were determined. Chi2 statistics was performed to test the association of HPV16 variants between case and control groups. The prevalence of HR-HPV types in

  15. Multiband mucosectomy in treatment of early cancer and precancerous lesions of esophagogastric junction%多环黏膜套扎切除术治疗食管胃交界部早癌及癌前病变的初步评价

    Institute of Scientific and Technical Information of China (English)

    邓彬; 吴大成; 王梅; 陈姚生; 王远志; 吴健; 丁岩冰

    2014-01-01

    回顾性分析2013年1月至2014年2月在扬州市第一人民医院接受多环黏膜套扎切除术(MBM)治疗的18例食管胃交界部(EGJ)早癌及癌前病变患者的临床资料,总结治疗效果及术后并发症发生情况.结果显示,18例患者均顺利完成手术,平均手术操作时间35.8 min.3例术中出血,内镜下成功止血;1例术中发生微小穿孔,以金属夹夹闭痊愈.术后病理诊断:5例为黏膜内癌,8例为高级别上皮内瘤变,5例为低级别上皮内瘤变.随访期间未见复发病例.提示,MBM可有效治疗EGJ早癌及癌前病变,具有安全、操作简便的优点.%Eighteen patients with early cancer and precancerous lesions of esophagogastric junction underwent multiband mucosectomy (MBM) in Yangzhou First People's Hospital from January 2013 to February 2014.The clinicopathological data of patients were analyzed retrospectively and the short-term efficacy and safety of MBM were evaluated.Operations were successful in all 18 cases.The mean operative time was 35.8 min.Intraoperative bleeding occurred in 3 cases and successfully managed by endoscopic hemostasis.Small perforation occurred in 1 patient and was closed by metal clips.Pathological examination showed mucosal cancer in 5 cases,high-grade intraepithelial neoplasia in 8 cases and low-grade intraepithelial neoplasia in 5 cases.No relapse of cancer was found during follow-up.Results indicate that MBM is an effective and safe method in treatment of early cancer and precancerous lesions in the esophagogastric junction.

  16. Risk assessment on liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in the diagnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    Ling-Zhi Chen

    2016-01-01

    Objective: To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression difference and significance of human papilloma virus L1(HPV L1) protein in exfoliative cells of different cervical lesions. Methods: A total of 1 400 cases of patients in gynaecological clinics of our hospital from January 2009 to August 2013 were selected and were given liquid based cytology test(LCT) examination and HPV L1 protein expression detection in exfoliative cells. Meanwhile, cervical biopsy pathology results were taken as baseline for make a comparison. Results: (1) In such1400 cases of women: LCT(+)-30 cases, accounting for 2.1%, HPV L1 protein detection(+)-18 cases, accounting for 1.29%; LCT and HPV L1 protein detection(+)-8 cases, LCT and HPV L1 protein detection(-)- 1360 cases. (2)Pathological tissue biopsy was performed on 30 cases of patients with LCT(+), results showed that(+)were 23 cases; pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that (+)were 14 cases. Among 1 360 cases with LCT and HPV L1 protein detection(-), 497 cases were willing to accept the pathological biopsy, and the results showed that 1 case presented(+), accounting for 0.2%. Total 545 cases were willing to accept the pathological biopsy, among whom, 38 cases presented(+), accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection, and difference had statistical significance. Conclusion: liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical lesions, and were expected as the effective method in the early clinical screening of cervical neoplasia lesions.

  17. Prevalence of cervical intraepithelial neoplasia grades II/III and cervical cancer in patients with cytological diagnosis of atypical squamous cells when high-grade intraepithelial lesions (ASC-H cannot be ruled out

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    Andréa Cytryn

    Full Text Available CONTEXT AND OBJECTIVE: The latest update of the Bethesda System divided the category of atypical squamous cells of undetermined significance (ASCUS into ASC-US (undetermined significance and ASC-H (high-grade intraepithelial lesion cannot be ruled out. The aims here were to measure the prevalence of pre-invasive lesions (cervical intraepithelial neoplasia, CIN II/III and cervical cancer among patients referred to Instituto Fernandes Figueira (IFF with ASC-H cytology, and compare them with ASC-US cases. DESIGN AND SETTING: Cross-sectional study with retrospective data collection, at the IFF Cervical Pathology outpatient clinic. METHODS: ASCUS cases referred to IFF from November 1997 to September 2007 were reviewed according to the 2001 Bethesda System to reach cytological consensus. The resulting ASC-H and ASC-US cases, along with new cases, were analyzed relative to the outcome of interest. The histological diagnosis (or cytocolposcopic follow-up in cases without such diagnosis was taken as the gold standard. RESULTS: The prevalence of CIN II/III in cases with ASC-H cytology was 19.29% (95% confidence interval, CI, 9.05-29.55% and the risk of these lesions was greater among patients with ASC-H than with ASC-US cytology (prevalence ratio, PR, 10.42; 95% CI, 2.39-45.47; P = 0.0000764. Pre-invasive lesions were more frequently found in patients under 50 years of age with ASC-H cytology (PR, 2.67; 95% CI, 0.38-18.83; P = 0.2786998. There were no uterine cervical cancer cases. CONCLUSION: The prevalence of CIN II/III in patients with ASC-H cytology was significantly higher than with ASC-US, and division into ASC diagnostic subcategories had good capacity for discriminating the presence of pre-invasive lesions.

  18. [Expression of TREM-1 in patients with invasive cervical cancer and precursor lesions].

    Science.gov (United States)

    Anaya-Prado, Roberto; Norzgaray-Ibarra, Fabiola Geovanna; Bravo-Cuéllar, Alejandro; Pérez-Avila, Carlos Eduardo; Schadegg-Peña, Daniel; Anaya-Fernández, Michelle M

    2015-01-01

    Introducción: se ha demostrado que la glicoproteína TREM-1 pertenece a la superfamilia de las inmunoglobulinas que induce la secreción de varias citocinas proinflamatorias. El objetivo de este trabajo fue medir la expresión de TREM-1 en pacientes con cáncer cervical. Métodos: en este estudio transversal analítico incluimos 4 grupos de pacientes: GI: mujeres con lesión intraepitelial (LI) escamosa de bajo grado (n = 15 p/g); GII: pacientes con LI escamosa de alto grado (n = 9 p/g); GIII: pacientes con cáncer cervical invasor (n = 9 p/g), y GIV: pacientes sanas (n = 15 p/g). En todas las pacientes se midió la expresión de TREM-1 y el Índice Medio de Fluorescencia (IMF) en neutrófilos y monocitos, así como los niveles de leucocitos, neutrófilos y monocitos. Usamos t de Student para muestras independientes. Para estas mismas variables, aplicamos prueba de suma de rangos de Mann-Whitney, ANOVA y Turkey. Para las variables cualitativas se utilizó la prueba de Chi cuadrada. Resultados: los porcentaje de expresión de TREM-1 en neutrófilos y monocitos, además del IMF en neutrófilos en los 4 grupos, no fue significativamente diferente. El IMF de TREM-1 en monocitos fue significativamente diferente al comparar el grupo II y grupo III frente al grupo IV (p grupos. Conclusión: este estudio documenta una mayor expresión de TREM-1 en monocitos de pacientes con cáncer avanzado.

  19. Application of Liquid Based Cytology Test on Early Screening of Cervical Precancerous Pathological Changes%液基细胞学检测在宫颈癌早期筛查中的价值

    Institute of Scientific and Technical Information of China (English)

    孙翠琴; 范洪斌

    2012-01-01

      Objective:To describe the value of Liquid-based cytology test(TCT)on early screening of cervical intraepithelial neoplasia.Method:The behesda system(TBS)was used to analyze the TCT of 625 patients.Colposcopy was performed on 126 cases,including 76 positive patients and 50 clinic highly questionable cases.So that to study the value of TCT in the clinical context and research the age distribution characteristics and clinical features of the patients.Result:According to TBS test,divide into:40 ASCUS,4 AGUS,3 ASC-H,18 LSIL,7 HSIL,3 SCC and 1 AC.The agreement rate of cytology and vaginal microscopically living tissue diagnosis in LSIL,HSIL and SCC each are 72.22%,85.71%and 100%.There are 4.64%cases in the 76 patents were with cervical intraepithelial neoplasia.The study shows that,females 31 to 40 years old are the higher age level of cervical intraepithelial neoplasia,about 46.05%.Conclusion:TCT are useful in early detection and treatment of cervical intraepithelial neoplasia.%  目的:探讨液基细胞技术在宫颈癌早期筛查中的应用价值.方法:对625例患者行TCT,用TBS诊断方法进行分析,对76例细胞学阳性及临床上高度可疑的50例共计126例患者行阴道镜检查,以评价TCT在临床上的应用价值,同时调查他们的疾病构成及年龄分布特征.结果:TBS诊断ASCUS 40例,AGUS 4例,ASC-H 3例,LSIL 18例,HSIL 7例,SCC 3例,AC 1例.细胞学与阴道镜下活组织病理诊断符合率分别为LSIL 72.22%,HSIL 85.71%,SCC 100%,76例患者中宫颈病变占4.64%,31~40岁的妇女为宫颈病变的最高发年龄段,占46.05%.结论:采用TCT技术对早期发现与治疗宫颈病变及宫颈癌有积极的临床作用.

  20. Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil

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    Prado José CM

    2010-04-01

    Full Text Available Abstract Background Human Papillomavirus (HPV is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation. Findings Of the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1% were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18. Conclusions Cervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts. Findings The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher

  1. Priority Setting for Improvement of Cervical Cancer Prevention in Iran

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    Azam Majidi

    2016-04-01

    Full Text Available Background Cervical cancer is the fourth most common cancer among women worldwide. Organized cervical screening and vaccination against human papilloma virus (HPV have been successful interventions for prevention of invasive cervical cancer (ICC. Because of cultural and religious considerations, ICC has low incidence in Iran and many other Muslim countries. There is no organized cervical screening in these countries. Therefore, ICC is usually diagnosed in advanced stages with poor prognosis in these countries. We performed a priority setting exercise and suggested priorities for prevention of ICC in this setting. Methods We invited experts and researchers to a workshop and asked them to list important suggestions for ICC prevention in Iran. After merging similar items and removing the duplicates, we asked the experts to rank the list of suggested items. We used a strategy grid and Go-zone analysis to determine final list of priorities for ICC prevention in Iran. Results From 26 final items suggested as priorities for prevention of ICC, the most important priorities were developing national guidelines for cervical screening and quality control protocol for patient follow-up and management of precancerous lesions. In addition, we emphasized considering insurance coverage for cervical screening, public awareness, and research priorities, and establishment of a cervical screening registry. Conclusion A comprehensive approach and implementation of organized cervical screening program is necessary for prevention of ICC in Iran and other low incidence Muslim countries. Because of high cost for vaccination and low incidence of cervical cancer, we do not recommend HPV vaccination for the time being in Iran.

  2. Trimodal spectroscopy as a tool for detecting cervical squamous intraepithelial lesions in vivo

    Science.gov (United States)

    Georgakoudi, Irene; Sheets, Ellen E.; Crum, Christopher P.; Mueller, Markus G.; Backman, Vadim; Feld, Michael S.

    2001-10-01

    Using intrinsic fluorescence, diffuse reflectance and light scattering spectroscopy we extracted quantitative biochemical and morphological information in vivo about the bulk tissue and the epithelial cell nuclei of ectocervical sites from 44 patients. Significant changes were observed in tissue morphology and biochemistry between normal, squamous metaplastic and squamous intraepithelial lesion (SIL) sites. The combined use of all three spectroscopic techniques (tri-modal spectroscopy) yielded superior results for detecting SILs than any one of the techniques alone.

  3. p16INK4 expression in precursor lesions of squamous cell cervical cancer related to the presence of HPV-DNA

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    A.E.G. Godoy

    2008-07-01

    Full Text Available The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both in the group consisting of cervical intraepithelial neoplasia (CIN1/HPV specimens and in the group of CIN2/CIN3 specimens with P value of 0.0001. p16INK4 expression was 48.3% in the CIN1/HPV group, as opposed to 94.3% in the CIN2/CIN3 group (P = 0.001, showing a statistically significant difference between the two groups. The quantitative method used here is simple and less subjective than the different semiquantitative methods described in the literature. In view of the different definitions of a p16INK4-positive case, it is almost impossible to compare the findings reported by different investigators. This study confirms the association between p16INK4 and CIN2 and CIN3 lesions. Moreover, it shows that some low grade lesions expressed high levels of this protein. This may indicate that such low grade lesions may be predisposed to progress to high grade lesions. This means that p16INK4 may be a strong marker for "neoplastic lesions" induced by HPV and not just an infection marker.

  4. 不同胃黏膜病变中iNOS表达与Hp的关系%The relationship between iNOS expression and Helicobacter pylori infection in gastric cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    雷琳; 黄亚平; 龚文容; 朱庆茹

    2013-01-01

    目的:检测诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在胃癌前病变及胃癌的表达,及其与幽门螺杆菌(Helicobacter pylori,Hp)感染的相关性,探讨Hp感染与胃癌发生的关系.方法:收集慢性浅表性胃炎(chronic superficial gastritis,CSG),慢性萎缩性胃炎伴肠上皮化生(Intestinal metaplasia,IM),慢性萎缩性胃炎伴上皮内瘤变(Intraepithelial neoplasia,IN)各30例,胃癌(gastric cancer,GC)40例.采用SP免疫组化法检测iNOS蛋白的表达,warthin-starry银染法检测胃黏膜Hp感染.结果:CSG、IM、IN、GC组织中iN-OS表达呈逐渐增强趋势,差异具有显著性(P<0.05).结论:CSG、IM、IN、GC组织中Hp感染的胃黏膜组织中,iNOS的表达高于无Hp感染的患者.%Objeetive:To explore the relationship of inducible nitric oxide synthase and Helicobacter pylori (Hp)infection in the pathogenesis of gastric cancer.Methods:The expression of iNOS was determined by immunohistochemical method in 130 gastric specimens including 30 cases of chronic superficial gastritis,30 cases of intestinal metaplasia,30 cases of intraepithelial neoplasia and 40 cases of gastric carcinomas.Results:In control group with chronic superficial gastritis,precancerous group,and gastri carcinoma group,the expression levels of iNOS had a increasing tendency (P < 0.05).Conclusion:The expression of iNOS in Hp positive group is significantly higher than that in negative group.(P < 0.05).

  5. Serum lipid profile in patients with oral cancer and oral precancerous conditions

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    Rajul Mehta

    2014-01-01

    Full Text Available Background: The present study was undertaken to estimate and compare the levels of plasma total cholesterol (TC, low density lipoprotein (LDL, high density lipoprotein (HDL, very low density lipoprotein (VLDL and triglycerides in patients with oral precancerous lesions/conditions, oral cancer and normal subjects. Materials and Methods: The study comprised of 60 patients with oral precancerous lesions/conditions, 60 patients with oral cancer and a control group of 60 healthy individuals. The diagnosis of oral precancerous lesions/conditions and oral cancer was confirmed histopathologically. Under aseptic condition 5 ml venous blood of overnight fasting patient was withdrawn from each individual. Serum was separated by centrifugation and plasma levels of TC, LDL, HDL, VLDL and triglycerides were estimated. Descriptive statistical analysis has been carried out in the present study. Analysis of variance has been used to find the significance of study parameters between three or more groups of patients, Post-hoc test as Tukey has been used to find the pair wise significance. Significance is assessed at 5% level of significance. Results: Statistically significant decrease in levels of plasma TC, LDL, HDL, VLDL and triglycerides was observed in the precancerous and cancerous groups as compared to the control group. On comparison between precancerous and cancerous groups, significant decrease was observed in cancerous group. Conclusion: The change in lipid levels may have an early diagnostic or prognostic role in the oral premalignant lesions/conditions and oral cancer. The presence of decreased plasma lipid profile should increase the suspicion of these lesions to be investigated further.

  6. A COMPARATIVE ANALYSIS OF SINGLE AND COMBINATION FEATURE EXTRACTION TECHNIQUES FOR DETECTING CERVICAL CANCER LESIONS

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    S. Pradeep Kumar Kenny

    2016-02-01

    Full Text Available Cervical cancer is the third most common form of cancer affecting women especially in third world countries. The predominant reason for such alarming rate of death is primarily due to lack of awareness and proper health care. As they say, prevention is better than cure, a better strategy has to be put in place to screen a large number of women so that an early diagnosis can help in saving their lives. One such strategy is to implement an automated system. For an automated system to function properly a proper set of features have to be extracted so that the cancer cell can be detected efficiently. In this paper we compare the performances of detecting a cancer cell using a single feature versus a combination feature set technique to see which will suit the automated system in terms of higher detection rate. For this each cell is segmented using multiscale morphological watershed segmentation technique and a series of features are extracted. This process is performed on 967 images and the data extracted is subjected to data mining techniques to determine which feature is best for which stage of cancer. The results thus obtained clearly show a higher percentage of success for combination feature set with 100% accurate detection rate.

  7. Detection, management, and follow-up of pre-malignant cervical lesions and the role for human papillomavirus.

    NARCIS (Netherlands)

    Hamont, D. van; Bekkers, R.L.M.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2008-01-01

    Cervical cytological pathology is common. Prevention of cervical cancer by detecting the disease process at an early and pre-malignant stage is practised globally either through population-based screening programmes (PSP) or through non-organised ones. High-grade cervical intraepithelial neoplasia (

  8. 宫颈疾病患者人乳头状瘤病毒感染分析%Human papilloma virus infections in patients with cervical lesion

    Institute of Scientific and Technical Information of China (English)

    蔡色霞

    2012-01-01

    OBJECTIVE To study the relationship between the human papilloma virus(HPV) infections and cervical lesions so as to provide basis for the prevention and control of cervical lesions. METHODS A total of 60 patients with various cervical lesions who enrolled Maternal and Child Health Hospital of Xianyou from Jul 2006 to Jun 2011 were chosen as the study objects, the condition of HPV infections in those patients was analyzed with pathological diagnosis. RESULTS Of 60 patients with cervical lesions, there were 37 cases with chronic cervicitis, the incidence of HPV infection was 29. 7% ; there were 23 patients with cervical intraepithelial neoplasia (CIN), including 10 cases of CIN I , 7 cases of CIN II , and 6 cases of CIN III ; the infection rates were 29. 7%, 60. 0% , 71. 4% , and 83. 3% , respectively. The infection rate of the patients with CINII or CIN III was significantly higher than that of the patients with CIN or cervicitis higher than that in the cases with CIN I and chronic eervicitis (P< 0.05). CONCLUSION The outbreak and development of cervical lesions are related to the HPV infections, the incidence of HPV infection increases significantly as aggravating the lesions; the detection of HPV can be applied in the screening of cervical lesions and the follow-up after the treatment.%目的 探讨人乳头状瘤病毒(HPV)感染与宫颈疾病发生的关系,为宫颈疾病的预防及其治疗提供依据.方法 分析福建省仙游县妇幼保健院2006年7月-2011年6月60例不同类型宫颈疾病患者HPV的感染情况,结合病理诊断进行分析.结果 60例宫颈病变患者中宫颈炎37例,HPV感染率为29.7%;宫颈上皮内瘤变(CIN)23例,其中CINⅠ10例、CINⅡ7例、CINⅢ6例,感染率分别为60.0%、71.4%、83.3%,中高度CIN( CINⅡ、CINⅢ)感染率明显高于低度CIN和宫颈炎(P<0.05).结论 宫颈病变的发生、发展与HPV感染有关,随着病变程度的加重,HPV感染率明显增高,HPV检测可用于宫颈

  9. 基于标本兼治的胃癌前病变临床疗效评价方法探讨%Study on Method for Clinical Effectiveness Evaluation of Precancerous Lesion of Gastric Cancer Treated with Addressing Both the Symptoms and Root Causes

    Institute of Scientific and Technical Information of China (English)

    沈舒文; 宇文亚; 李周利; 黄毓娟; 洪亚庆

    2011-01-01

    Addressing both the symptoms and root causes means regulating structure of sufficient-syndrome and deficiency-syndrome on the basis of differential treatment, which is the therapeutic advantage of Traditional Chinese Medicine (TCM) in treating precancerous lesion of gastric cancer. However, up to now ,the scientific method of clinical effectiveness evaluation for addressing both symptoms and root causes is absent. In this paper, we explored the method by modern mathematics,statistics methods and techniques, combined with its own characteristics of TCM.%标本兼治是辨证论治层位上的虚实证候结构调治,也正是中医治疗胃癌前病变的疗效优势所在,但对其疗效评价尚缺乏科学的方法.充分利用现代数学、统计学方法与技术,结合中医药自身的特点,探索基于标本兼治的胃癌前病变临床疗效评价方法.

  10. The purpose of cervical cells by TBS + DNA quantitative detection in cervical lesions%宫颈细胞TBS+DNA倍体定量检测在宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    樊精湛

    2009-01-01

    Objective:The purpose of cervical cells by TBS + DNA ploidy quantitative detection, early detection of cervi-cal lesions, blocked the occurrence of cervical cancer. Methods: Gynecology clinics of 1 000 eases of cervical cells, women in TBS + DNA ploidy quantitative tests in patients with positive cervical biopsy done. Diagnostic criteria for the use of TBS classification systems. Results: 1 000 cases of women in cervical serape specimens films, TBS + DNA ploidy in 53 cases of positive quantitative detection, the positive rate was 5.3%, in which atypical glandular cells (AGCUS) 2 cases, low-grade squamous intraepithelial lesion (LSIL) 40 cases, a high degree of squamous intraepithelial lesion (HSIL) 9 cases of squamous cell carcinoma (SCC) 2 cases. Positive for 53 eases of cervical biopsy, which mild atypical glandular hyper-plasia and 2 eases, CIN Ⅰ 39 cases, CIN Ⅱ 7 cases, CIN Ⅲ 3 cases, scc 2 cases. Cervical biopsy of TBS + DNA ploidy provide positive detection of lesions to be diagnosed. Ages 30-40 years of age is the age of high incidence of CIN, the probability was 30/53 (56.6%). Conclusion:Cervical cells TBS+DNA ploidy and quantitative determination of some histological correlation. TBS+DNA quantitative determination of ploidy as a non-invasive detection of out-patient screening is an effective method for cervical cancer. Screening for cervical cancer patient to pay special attention to young women.%目的:通过对宫颈细胞TBS+DNA倍体定量检测,及早发现宫颈病变,阻断官颈癌的发生.方法:对妇科门诊就诊的1 000例妇女进行宫颈细胞TBS+DNA倍体定量检测,阳性患者再做官颈活检.诊断标准采用TBS分类系统.结果:1 000例妇女官颈刮片标本,TBS+DNA倍体定量检测阳性53例,阳性率为5.3%,其中不典型腺细胞(AGCUS)2例,低度鳞状上皮内病变(LSIL)40例,高度鳞状上皮内病变(HSIL)9例,鳞状细胞癌(SCC)2例.53例阳性者进行宫颈活检,其中轻度不典型腺上皮增生2

  11. Cytohistological correlation and accuracy of the pap smear test in diagnosis of cervical lesions: a hospital based cross-sectional study from Odisha, India

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    Reena Naik

    2015-09-01

    Full Text Available Background Cervical carcinoma is one of the most common causes of mortality among women.Early detection can be done by Papanicolaou (Papsmear test - a simplest, safe, cost effective and non invasive procedure. The main objective of this study was to categorize Pap smears for cytohistologicalexamination as well as clinically correlate to analyze sensitivity, specificity and positive predictive value of Pap smear. Methods In this prospective study 417 Pap smears performed, cytohistological correlation was done in 104 cases because patients had undergone both Pap smear and cervical biopsy.Detailed history was taken and clinical examination was done. Pap smear sample was collected from cervix and reporting was made according to Bethesda 2001 classification.Cytological findings were correlated with histopathology. Results Overall concordance rate was 60.7%. Concordance rate for malignancy was 100%, for inflammatory lesions 70.8% and for cervical intraepithelial neoplasia (CIN 33.3%. Common age group presented for screening was40-50 years.Commonest clinical presentationwas bleeding per vaginum. Conclusion The study provides good cyto-histopathology correlation in detecting high grade lesions and malignancy. Although Pap smear sensitivity was low but can be increased by adequate sampling and avoiding technical errors.Bethesda system is strongly recommended for adequacy of sampling to minimize inconsistency.Regular screening should be advised to the patients for the early detection of cervical carcinoma.

  12. Analysis of papillomavirus infections in patients with cervical lesions%宫颈病变患者人乳头状瘤病毒感染调查分析

    Institute of Scientific and Technical Information of China (English)

    蓝允莲; 王岩; 郑丽萍

    2015-01-01

    OBJECTIVE To investigate papillomavirus infections in patients with cervical lesions ,and provide guid‐ance for prevention of aggravation of cervical lesions .METHODS Totally 165 patients with cervical diseases in the obstetrics and gynecology department were enrolled ,including 44 patients with cervical cancer ,56 patients with cervical intraepithelial neoplasia and 65 patients with cervicitis/cervical condyloma acuminatum .The analysis of HPV subtype was conducted on the basic chip samples by flow‐through hybridization technique .RESULTS HPV infection occurred in 116 out of 165 cases of cervical lesions ,the infection rate was 70 .3% .Among them ,there were 31 cases of high risk HPV infection (the infection rate 47 .7% ) and 10 cases of low risk HPV infection (15 .4% ) in patients with cervicitis/condyloma acuminatum ,35 cases of high risk HPV infection (62 .5% ) and 4 cases of low risk HPV (7 .1% ) in patients with cervical intraepithelial neoplasia;36 cases of high risk HPV infec‐tion (81 .8% ) and 0 cases of low risk HPV infection in cervical cancer patients .The results showed that there was significance in the rate of high and low HPV infection between patients with different cervical lesions (P<0 .05) . There were 39 cases of single infection (the infection rate 60% ) and 8 cases of multiple infections (12 .3% ) in pa‐tients with cervicitis/condyloma acuminatum ,31 cases of single infection (55 .4% ) and 9 cases of multiple infec‐tions (16 .1% ) in patients with cervical intraepithelial neoplasia ,29 cases of single infection (65 .9% ) and 7 cases of multiple infections (15 .9% ) in cervical cancer patients .CONCLUSION Multiple high risk HPV infection is closely related to cervical lesions ,and the treatment should be based on the detection results of HPV subtypes of patients with cervical lesions so as to timely prevent and treat cervical cancer .%目的:对宫颈病变患者人乳头状瘤病毒(H PV )感染调查分析,为预防宫颈

  13. Elimination of Specular reflection and Identification of ROI: The First Step in Automated Detection of Cervical Cancer using Digital Colposcopy

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    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, Specular Reflections (SR) appear as bright spots heavily saturated with white light. These occur due to the presence of moisture on the uneven cervix surface, which act like mirrors reflecting light from the illumination source. Apart from camouflaging the actual features, the SR also affects subsequent segmentation routines and hence must be removed. Our novel technique eliminates the SR and makes the colposcopic images (cervigram) ready for segmentation algorithms. The cervix region occupies about half of the cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. The first step is, therefore, focusing on the cervical bor...

  14. Bulky Early-Stage Cervical Cancer (2-4 cm Lesions): Upfront Radical Trachelectomy or Neoadjuvant Chemotherapy Followed by Fertility-Preserving Surgery: Which Is the Best Option?

    Science.gov (United States)

    Plante, Marie

    2015-05-01

    Radical trachelectomy is now recognized as a valid treatment option for young women with early-stage cervical cancer with lesions measuring less than 2 cm. However, for women with bulky lesions measuring greater than 2 cm, few data are available in the literature to guide management. There are currently 2 options available: either upfront radical trachelectomy or neoadjuvant chemotherapy followed by fertility-preserving surgery. Overall, both options offer very good oncologic outcome; however, the rate of fertility preservation and obstetrical outcome seem superior after neoadjuvant chemotherapy. Advantages and disadvantages of both options are discussed and a thorough literature review is provided. Issues to be further studied are also outlined.

  15. A placebo-controlled randomized HPV16 synthetic long-peptide vaccination study in women with high-grade cervical squamous intraepithelial lesions

    OpenAIRE

    de Vos van Steenwijk, Peggy J.; Ramwadhdoebe, Tamara H.; Löwik, Margriet J. G.; van der Minne, Caroline E.; Berends-van der Meer, Dorien M A; Fathers, Lorraine M; Valentijn, A. Rob P. M.; Oostendorp, Jaap; Fleuren, Gert Jan; Hellebrekers, Bart W. J.; Welters, Marij J. P.; van Poelgeest, Mariette I.; Melief, Cornelis J. M.; Kenter, Gemma G; van der Burg, Sjoerd H.

    2012-01-01

    The aim of this study was to investigate the capacity of an HPV16 E6/E7 synthetic overlapping long-peptide vaccine to stimulate the HPV16-specific T-cell response, to enhance the infiltration of HPV16-specific type 1 T cells into the lesions of patients with HPV16+ high-grade cervical squamous intraepithelial lesion (HSIL) and HPV clearance. This was a placebo-controlled randomized phase II study in patients with HPV16-positive HSIL. HPV16-specific T-cell responses were determined pre- and po...

  16. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists.

    Science.gov (United States)

    Bomfim, Rafael Aiello; Crosato, Edgard; Mazzilli, Luiz Eugênio Nigro; Frias, Antonio Carlos

    2015-01-01

    This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs) in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed) were evaluated in a Centro de Referência em Saúde do Trabalhador - CEREST (Worker's Health Reference Center). The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p smoking (p = 0.01) and age group (p = 0.04). The prevalence of NCCLs in the study population was particularly high (76.84%), and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  17. Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV Found in Cervical Lesions of Women from North-East Brazil

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    Ana Pavla Almeida Diniz Gurgel

    2013-01-01

    Full Text Available The aim of this study was to examine the prevalence and genetic variability of the capsid L1 gene of rare HPV genotypes that were found in the cervical lesions of women from North-East Brazil. A total number of 263 patients were included in this study. HPV detection was performed using PCR followed by direct sequencing of MY09/11, as well as type-specific PCR to detect the Alpha-9 species. Epitope prediction was performed to determine whether or not the genetic variants are inserted in B-cell and T-cell epitopes. The prevalence of rare HPV types in cervical lesions was found to be 9.47%. The rare HPV genotypes that were detected were HPV-53, 54, 56, 61, 62, 66, 70, and 81. The genetic variability in the L1 gene of rare HPV types involved thirty nucleotide changes, eight of which were detected for the first time in this study. Moreover, some of these variants are embedded in B-cell or T-cell epitope regions. The results of this research suggest that rare HPV types might be involved in cervical lesions and some of these variants can be found in B-cell and T-cell epitopes. Data on the prevalence and variability of rare HPV types will assist in clarifying the role of these viruses in carcinogenesis.

  18. Co-infections associated with human immunodeficiency virus type 1 in pregnant women from southern Brazil: high rate of intraepithelial cervical lesions

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    Michele Tornatore

    2012-03-01

    Full Text Available Human immunodeficiency virus type 1 (HIV-positive pregnant women require specific prophylactic and therapeutic approaches. The efficacy of established approaches is further challenged by co-infection with other sexually transmitted diseases (STDs. The objective of this study was to determine the prevalence of co-infections in pregnant women infected with different HIV-1 subtypes and to relate these findings, together with additional demographic and clinical parameters, to maternal and infant outcomes. Blood samples from pregnant women were collected and tested for syphilis, hepatitis B virus (HBV and hepatitis C virus (HCV. Human papillomavirus (HPV diagnosis was evaluated by the presence of alterations in the cervical epithelium detected through a cytopathological exam. Medical charts provided patient data for the mothers and children. Statistical analyses were conducted with STATA 9.0. We found a prevalence of 10.8% for HCV, 2.3% for chronic HBV, 3.1% for syphilis and 40.8% for HPV. Of those co-infected with HPV, 52.9% presented high-grade intraepithelial lesions or in situ carcinoma. Prematurity, birth weight, Apgar 1' and 5' and Capurro scores were similar between co-infected and non-co-infected women. The presence of other STDs did not impact maternal and concept outcomes. More than half of the patients presenting cervical cytology abnormalities suggestive of HPV had high-grade squamous intraepithelial lesions or cervical cancer, evidencing an alarming rate of these lesions.

  19. Relationship of TLR4 expression and H.Pylori infection in gastric carcinoma and precancerous lesions%胃癌及癌前病变中TLR4的表达及与幽门螺杆菌感染的关系

    Institute of Scientific and Technical Information of China (English)

    王强; 薛乐宁; 张维; 陆建云; 陆文; 林敏; 王晓勇

    2011-01-01

    Objective To investigate the relationship of toll like receptor 4(TLR4) expression and H. pylori infection in gastric carcinoma and precancerous lesions. Methods TLR4 expression was detected by immunohistochemical method in gastric carcinoma(44 cases) and on its precursor lesions,dysplasia ( 35 cases ), intestinal metaplasia ( 36 cases ) and chronic gastritis ( 41 cases ) by immunohistochemistry. Giemsa staining method was performed for H. pylori infection. Results Gastric epithelium with chronic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma all expressed TLR4. Conclusion Expression of TLR4 enables gastric epithelium cells to interact with H. pylori.%目的 探讨TLR4蛋白在胃癌及癌前病变组织中表达及与幽门螺杆菌感染的关系.方法 免疫组化SP法检测慢性胃炎(CSG)41例、肠上皮化生(IM) 36例、不典型增生(Dys)35例和胃癌(GC)44例标本中TLR4蛋白的表达,Giemsa染色法检测幽门螺杆菌.结果 TLR4在CSG、IM、Dys、GC胃黏膜中均有表达.在幽门螺杆菌阳性组中,TLR4蛋白表达明显高于幽门螺杆菌阴性组[CSG,(66.7%vs 33.3%);IM,(63.0%vs 37.0%);Dys,(57.7%vs 42.3%);GC,(61.8%vs.38.2%)](P<0.05).结论 TLR4蛋白与幽门螺杆菌感染相互作用导致胃黏膜病变.

  20. MRI Technique in the Diagnosis of Cervical Cystic Lesions%上颈部囊性病变的MRI分析

    Institute of Scientific and Technical Information of China (English)

    杨宁; 刘丽辉

    2014-01-01

    目的:探讨MRI检查在上颈部囊性病变中的诊断价值。方法:36例上颈部囊性肿物,术前行穿刺检查和MRI检查以明确囊液性质、嚢肿范围、来源及信号强度。根据囊液性质及MRI检查特点,决定手术方法,术后病理确诊。结果:穿刺囊液为蛋清样黏液23例,为淡黄色水样液体6例,为灰色脓汁样液体5例。另有2例患者不配合未能行穿刺检查。MRI检查T1低信号、T2高信号且囊肿有“尾状征”与口底关系密切者25例,诊断为潜突型舌下腺囊肿,采用口内入路摘除舌下腺,其余11例采用颈部入路。术后病理证实潜突型舌下腺囊肿25例,淋巴管瘤6例,鳃裂囊肿5例。所有术后病理确诊与术前诊断相符,随访3~36月,未见有患者复发。结论:上颈部囊性病变术前MRI检查,有助于术前明确诊断,以正确指导手术,避免盲目颈部探查手术。%Objective: To explore the diagnostic value of MRI technique applied for cervical cystic lesions. Method: 36 cases with cervical cystic lesions were checked preoperatively by puncture examination and/or MRI. Results: There were 23 cases with the cystic fluid like egg white, 6 cases with pale yellow watery fluid and 5 cases with gray pus-like fluid. Another 2 patients didn't complete puncture examination because of poor cooperation. 25 MRI images clearly showed the cystic wall was originated from the ipsilateral sublingual gland, 11 patients without correct preoperative diagnosis by MRI. The patients with low signal T1 and hyperintensity signal T2 and "tail syndrome" in MRI were subject to using intraoral approach to remove the sublingual gland. There was no patient of recurrence within 3 to 36 months follow-up. The postop-erative pathology confirmed the preoperative diagnosis. Conclusion:MRI examination can clearly show the travel and ori-gin sites of cystic lesions of the neck and contribute to get correct diagnosis preoperatively.

  1. Risk factors for cervical carotid and intracranial cerebrovascular lesions in patients undergoing coronary artery bypass grafting. Preoperative evaluation using magnetic resonance imaging and angiography

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    Makino, Masahiro [Kyoto Prefectural Univ. of Medicine (Japan). Research Inst. for Neurological Diseases and Geriatrics

    2001-12-01

    Recently neurologic complications after coronary artery bypass grafting (CABG) have received increasing attention. There is no detailed report about the risk factors for these complications, although stenosis in the cervical and intracranial arteries, especially in Japanese patients, latent ischemic brain lesions and preoperative neurological conditions are related to these events. In this prospective study, we evaluated occlusive lesions in the cervical carotid and intracranial arteries, silent brain infarction and cerebral deep white matter lesion with MRA and MRI in patients scheduled to undergo CABG to determine the prevalence of occlusive diseases in cervical carotid and intracranial arteries, latent ischemic change in the brain in this population and to identify preoperative risk factors for these patients. The subjects were 144 consecutive patients (103 men and 41 women, mean age 65.9{+-}9.2 years old) who were scheduled for CABG under elective conditions and who were examined by the same MRI apparatus using the same protocol between November 1998 and March 2001. After routine neurological examination and Mini-Mental State Examination (MMSE) were completed, MRI and MRA were obtained, then the prevalence of abnormalities on MRI and MRA studies and risk factors were evaluated. Cervical carotid artery stenosis with {>=}50% luminal narrowing was detected in 29.2% of the subjects, and that with {>=}75% luminal narrowing was detected in 16.0% of the subjects. Intracranial arterial stenosis showing {>=}50% luminal narrowing was detected in 38.2% of subjects, and that showing {>=}75% luminal narrowing was detected in 19.4% of subjects. Brain infarction was observed in 74.3% of subjects, cerebral deep white matter lesion showing grade 2 or higher on Fazekas classification was observed in 17.4% of the subjects. The characteristics, including possible risk factors of subjects with and without these abnormal findings, were compared. Patients with cervical carotid

  2. 内镜下卢戈液染色联合窄带成像对食管癌及癌前病变的诊断分析%Diagnostic analysis of endoscopic Lugol solution staining combined with narrow band imaging for ;esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    杨彬; 陈滟

    2015-01-01

    目的:探讨内镜下卢戈液染色联合窄带成像(NBI)对食管癌及癌前病变的诊断价值。方法选择2013年12月至2014年12月在四川省遂宁市中心医院行胃镜检查,存在可疑食管病灶而进行卢戈液染色和病理学检查的187例患者作为对照组,选择2015年1月至2015年6月行胃镜检查,存在可疑食管病灶而进行卢戈液染色联合 NBI 检查和病理学检查的124例患者作为观察组。对两组检查的敏感性、特异性和准确性进行比较。结果观察组患者内镜下检测阳性率为75.0%,高于对照组的55.6%,差异有统计学意义(χ2=12.07,P <0.01)。观察组患者的食管癌和高级别内瘤变检出率分别为29.0%和17.7%,均高于对照组的18.2%和6.4%,且差异有统计学意义(χ2=4.15,P =0.04;χ2=8.54,P <0.01)。观察组患者内镜下检测结果与病理学检查结果的 Kappa 值=0.55,对照组患者的Kappa值=0.36。观察组检测方法的敏感性和准确性分别为89.9%和82.3%,高于对照组的71.8%和68.4%,且差异均有统计学意义(χ2=9.79,P <0.01;χ2=7.38,P <0.01)。结论卢戈液染色联合 NBI对食管癌和食管癌前病变检测的敏感性和检出率优于单纯性卢戈液染色方法,值得在早期食管癌及癌前病变诊断中进行推广。%Objective To investigate the diagnostic value of Lugol solution staining combined with nar-row band imaging (NBI)for esophageal cancer and precancerous lesions.Methods From December 201 3 to December 2014,1 87 patients who were with suspicious esophageal lesions and stained with Lugol solution under endoscopy were selected as control group,while from January 2015 to June 2015,124 patients who were with sus-picious esophageal lesions and examined with Lugol solution staining and NBI under endoscopy were selected as observation group.The sensitivity

  3. 宫颈环形电切术治疗宫颈病变的临床护理分析%Analysis of Clinical and Care of Cervical Loop Electrosurgical Excision Procedure for Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    王晓芬

    2012-01-01

    目的:探讨使用宫颈环形电切术(LEEP)治疗宫颈病变的临床疗效与护理体会.方法:选取在我院进行宫颈环形电切术治疗的110例宫颈病变患者,分为两组各55例:观察组给予针对性的护理干预,对照组采取常规护理,观察比较其临床治疗与护理效果.结果:观察组总有效率为92.7%,不良反应率为3.6%;对照组总有效率为74.5%,不良反应率为18.2%,两组差异显著具有统计学意义(P<0.05).结论:宫颈环形电切术治疗宫颈病变的临床效果显著,配合针对性的护理干预,能有效减少术后并发症的发生,提高治愈率.%Objective:To investigate the use of cervical loop electrosurgical excision procedure (LEEP) Clinical efficacy of treatment of cervical lesions and nursing.Methods:In our hospital for treatment of cervical loop electrosurgical excision procedure, 110 cases of cervical lesions, all 55 cases are divided into two groups: observation group was given specific nursing interventions to usual care control group, observed and compared the clinical treatment and care the effect.Results:The total effective rate was 92.7%, adverse reactions was 3.6%; the control group, the total effective rate was 74.5%, 18.2% rate of adverse reactions, significant difference between the two groups was statistically significant (P<0.05).Conclusion:The treatment of cervical loop electrosurgical excision of cervical lesions a significant clinical effect, with targeted nursing interventions can effectively reduce the incidence of postoperative complications and improve the cure rate.

  4. AN UPWARD TREND IN DNA P16INK4A METHYLATION PATTERN AND HIGH RISK HPV INFECTION ACCORDING TO THE SEVERITY OF THE CERVICAL LESION

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    Fernanda Nahoum Carestiato

    2013-09-01

    Full Text Available SUMMARY High-risk human papillomavirus (hr-HPV infection is necessary but not sufficient for cervical cancer development. Recently, P16INK4A gene silencing through hypermethylation has been proposed as an important cofactor in cervical carcinogenesis due to its tumor suppressor function. We aimed to investigate P16INK4A methylation status in normal and neoplastic epithelia and evaluate an association with HPV infection and genotype. This cross-sectional study was performed with 141 cervical samples from patients attending Hospital Moncorvo Filho, Rio de Janeiro. HPV detection and genotyping were performed through PCR and P16INK4A methylation by nested-methylation specific PCR (MSP. HPV frequency was 62.4% (88/141. The most common HPV were HPV16 (37%, HPV18 (16.3% and HPV33/45(15.2%. An upward trend was observed concerning P16INK4A methylation and lesion degree: normal epithelia (10.7%, low grade lesions (22.9%, high grade (57.1% and carcinoma (93.1% (p < 0.0001. A multivariate analysis was performed to evaluate an association between methylation, age, tobacco exposure, HPV infection and genotyping. A correlation was found concerning methylation with HPV infection (p < 0.0001, hr-HPV (p = 0.01, HSIL (p < 0.0007 and malignant lesions (p < 0.0001. Since viral infection and epigenetic alterations are related to cervical carcinoma, we suggest that P16INK4A methylation profile maybe thoroughly investigated as a biomarker to identify patients at risk of cancer.

  5. RARβ在宫颈癌及癌前病变中的表达调控和意义%The association of altered RARβ gene expression with the cervical lesion pathogenesis

    Institute of Scientific and Technical Information of China (English)

    Nafeisha Kadeer; Hui He; Abida Abudoukadeer; Anaguli Ababaikeli; Abulizi Abudula

    2011-01-01

    Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β (RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was to investigate the association of cervical cancer development with the RARβ gene expression at the mRNA and protein level to assess the impact of RARβ as a marker for early detection of the cancer.Methods:We collected 126 cases of formalin fixed and paraffin embedded cervical tissue specimens as well as 37 cases of fresh tissue samples from women with cervicitis,cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC).The RARβ mRNA and protein expression was detected by quantitative RT-PCR and immunohistochemistry,respectively.Results:(1) The mRNA expression of RARβ in CIN and cervical cancer was markedly decreased compared to cervicitis with a statistically very significant difference,but no difference was found between CIN and cervical cancer.(2) RARβ protein was normally expressed in the epithelial cells of cervicitis and partially lost in a few cases,but with the development of cervical lesion pathogenesis and cancer,a significant loss of protein expression was detected in CIN (38%) and CSCC (57%) compared to cervicitis (P < 0.01).Conclusion:The downregulation of RARβ transcription or loss of protein expression is an important indicator of cervical cancer and its precursur lesions.The detection of RARβ expression coupled with aberrant methylation of the gene may become a biomarker for the early prognosis or diagnosis of the cancer.

  6. SPECTRUM OF CERVICAL LESIONS IN CIMS, BILASPUR: A 5 YEAR RETROSPECTIVE STUDY OF 215 CASES IN A TERTIARY HOSPITAL OF CENTRAL INDIA

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    Sadhna

    2015-10-01

    Full Text Available BACKGROUND AND OBJEC TIVE: Cancer of the cervix is a leading cause of morbidity and mortality among women W orld W ide. Therefore to curb the disease there is need of awareness of this disease. This study is aimed to categorize the premalignant and malignant lesions at the earliest, to reduce the mortality and morbidity. It also aimed to know the incidence of malignancies at our set up and to calculate distribution of patient in relation to parity and symptoms. DESIGN AND SETTING: Study included 215 patients with complain of white dis charge per vagina, per vaginal bleeding and backache attending the gynecology OPD over a period of 05 years. PATIENT AND METHODS: Patients were subjected to cervical biopsy and biopsies were sent to pathology department. Detailed clinical history, age, age at marriage, parity and socio - economic status was obtained. RESULT: Result were classified histopathological l y as inflammatory lesions 70 cases, polypoidal lesion were 30, LSIL accounted for 32 cases and HSIL were 20 cases and 30 were malignant lesions. U nder malignant category moderately differentiated squamous cell carcinoma was the commonest. Maximum numbers of patients were subjected to cervical biopsies in 3 rd decade of life. Malignancies were common in 3 rd & 4 th decade of life. Most of the cases were of parity 4. CONCLUSION : Tissue biopsy is a valuable diagnostic procedure on which surgeons and radiologists still rely.

  7. Expression of E6, p53 and p21 proteins and physical state of HPV16 in cervical cytologies with and without low grade lesions.

    Science.gov (United States)

    Tagle, Diana K Jiménez; Sotelo, Daniel Hernández; Illades-Aguiar, Berenice; Leyva-Vazquez, Marco A; Alfaro, Eugenia Flores; Coronel, Yaneth Castro; Hernández, Oscar Del Moral; Romero, Luz Del Carmen Alarcón

    2014-01-01

    The aim of this study was to determine the correlation between expression of HPV16 E6, p53 and p21 proteins and the physical state of HPV16 in cervical cytologies without squamous intraepithelial lesions (Non-SIL) and with low grade squamous intraepithelial lesions (LSIL), both with HPV16 infection. 101 liquid-based cytological samples were analyzed. 50 samples were without squamous intraepithelial lesions (Non-IL) and 51 samples of low grade squamous intraepithelial lesions (LSIL), both with HPV16 infection. HPV16 infection was determined by PCR-RFLP, and the physical state of HPV16 by in situ hybridization with tyramide-amplification. The expression of E6, p53 and p21 proteins was evaluated by immunocytochemistry. The expression of HPV16 E6 protein was significantly higher in LSIL that in Non-SIL samples (p=0.006). We found a significant correlation between E6 expression and the physical state of HPV16 in Non-SIL (p=0.049). Our results suggest that high expression of E6 in LSIL is an early event of cervical carcinogenesis and perhaps can be used as an early marker.

  8. Stress Analysis of Occlusal Forces in Canine Teeth and Their Role in the Development of Non-Carious Cervical Lesions: Abfraction

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    Shihab A. Romeed

    2012-01-01

    Full Text Available Non-carious cervical tooth lesions for many decades were attributed to the effects of abrasion and erosion mainly through toothbrush trauma, abrasive toothpaste, and erosive acids. However, though the above may be involved, more recently a biomechanical theory for the formation of these lesions has arisen, and the term abfraction was coined. The aim of this study was to investigate the biomechanics of abfraction lesions in upper canine teeth under axial and lateral loading conditions using a three-dimensional finite element analysis. An extracted human upper canine tooth was scanned by μCT machine (Skyscan, Belgium. These μCT scans were segmented, reconstructed, and meshed using ScanIP (Simpleware, Exeter, UK to create a three-dimensional finite element model. A 100 N load was applied axially at the incisal edge and laterally at 45° midpalatally to the long axis of the canine tooth. Separately, 200 N axial and non-axial loads were applied simultaneously to the tooth. It was found that stresses were concentrated at the CEJ in all scenarios. Lateral loading produced maximum stresses greater than axial loading, and pulp tissues, however, experienced minimum levels of stresses. This study has contributed towards the understanding of the aetiology of non-carious cervical lesions which is a key in their clinical management.

  9. Histopathologic extent of cervical intraepithelial neoplasia 3 lesions in the atypical squamous cells of undetermined significance low-grade squamous intraepithelial lesion triage study: implications for subject safety and lead-time bias.

    Science.gov (United States)

    Sherman, Mark E; Wang, Sophia S; Tarone, Robert; Rich, Laurie; Schiffman, Mark

    2003-04-01

    Cervical intraepithelial neoplasia 3 (CIN3) is the precursor of mostsquamous carcinomas and serves as a surrogate end point. However, small CIN3 lesions are rarely associated with concurrent invasion. We hypothesized that aggressive follow-up for cytology of atypical squamous cells of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesion (LSIL) leads predominantly to detection of smaller CIN3 lesions than those usually associated with cancer. We assessed this hypothesis in a masked histopathologic review of 330 CIN3 lesions in the ASCUS LSILTriage Study, focusing on ASCUS referrals. ASCUS referrals underwent randomized management [colposcopy for repeat cytology of high-grade squamous intraepithelial lesion (HSIL), colposcopy for oncogenic human papillomavirus (HPV) detection or repeat HSIL, or immediate colposcopy]; then all were followed with repeat cytology for 2 years, followed by colposcopy and aggressive treatment. We assessed all CIN3 lesions qualitatively and measured 39 of them. CIN3 lesions were overwhelmingly small. Compared with enrollment, lesions found at follow-up or exit involved fewer tissue fragments (P < 0.01) and showed less diffuse gland involvement (P = 0.03). CIN3 lesions found postenrollment after HPV testing involved the fewest tissue fragments [versus immediate colposcopy (P = 0.04) or repeat cytology of HSIL (P = 0.02)], and none showed diffuse gland involvement. The median distal-proximal length was 6.5 mm (median replacement of total epithelium = 5%) in the 39 measured cases. We conclude that CIN3 lesions underlying ASCUS or LSIL generally lack features associated with invasion, particularly if managed using HPV testing, suggesting that aggressive management leads to early detection of CIN3 but probably prevents relatively few cancers in screened populations.

  10. GSTM1, GSTT1, and NQO1 polymorphisms in cervical carcinogenesis.

    Science.gov (United States)

    Nunobiki, Osamu; Ueda, Masatsugu; Akise, Hikari; Izuma, Shinji; Torii, Kiyo; Okamoto, Yoshiaki; Tanaka, Ichiro; Noda, Sadamu; Akashi, Kyoko; Higashida, Taro

    2015-07-01

    The aim of the study is to investigate the clinical significance of glutathione-S-transferase GSTM1, GSTT1, and NQO1 c.609C>T (rs1800566) genetic polymorphisms in cervical carcinogenesis. GSTM1, GSTT1, and NQO1 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 192 cervical smear in exfoliated cervical cell samples using polymerase chain reaction (PCR) system and real-time polymerase chain reaction (PCR) system. The 19 patients with high-grade squamous intraepithelial lesion had statistically higher frequency of null GSTT1 genotype than 9 with low-grade squamous intraepithelial lesion (LSIL) among the 67 patients with high-risk HPV (P = 0.024). The 24 patients with HSIL had also statistically higher frequency of NQO1 (CT+TT) genotype than 14 with LSIL among the 67 patients with high-risk HPV (P = 0.024). GSTT1 null and NQO1 genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population.

  11. 醋酸联合靛胭脂染色诊断早期胃癌及癌前病变%The clinical application of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    胡立华; 陈鹏; 张继晔; 王燕颖; 王文多; 张沛怡

    2014-01-01

    目的 探讨内镜下醋酸联合靛胭脂染色法对早期胃癌及癌前病变的临床诊断价值.方法 2011年1-12月在我院就诊患者行常规胃镜检查,发现可疑病灶者300例,随机分为两组,染色组(150例)行内镜下醋酸联合靛胭脂染色后活检,对照组(150例)行内镜下靛胭脂染色后活检.结果 染色组150例中共检出早期胃癌16例(10.7%)、重度不典型增生15例(10.0%)、中度不典型增生、肠上皮化生79例(52.7%),其余40例为胃炎.对照组150例中检出早期胃癌5例(3.3%)、重度不典型增生10例(6.7%)、中度不典型增生、肠上皮化生42例(28.0%),其余93例为胃炎.染色组早期胃癌及癌前病变检出率明显高于对照组,差异有统计学意义(P <0.001).醋酸联合靛胭脂染色后早期胃癌和重度不典型增生黏膜主要表现为黏膜退色,中度不典型增生和肠上皮化生黏膜主要表现为黏膜着色不良,慢性胃炎和正常黏膜主要表现为着色均匀.结论 内镜下醋酸联合靛胭脂染色可显著提高早期胃癌和癌前病变的检出率,具有较高的临床应用价值.%Objective To investigate the significance of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion.Methods After conventional electronic endoscopic diagnosis,From 2011 fanuay to 300 patients of suspicious lesions were randomly divided into endoscopic dye group and control group,each was 150 cases.Patients of endoscopic dye group were directly performed biopsy after acetic acid and indigo carmine dye,while in control group were performed biopsy after only indigo carmine dye.Results In the endoscopic dye group,16 early neoplasia (10.7%),15 severe atypical hyperplasia (10.0%),79 moderate atypical hyperplasia or Intestinal metaplasia (52.7%) and 40 chronic gastritis were found.In the control group,5 early neoplasia (3.3%),10 severe atypical hyperplasia (6.7%),42

  12. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists

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    Rafael Aiello BOMFIM

    2015-01-01

    Full Text Available This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed were evaluated in a Centro de Referência em Saúde do Trabalhador – CEREST (Worker’s Health Reference Center. The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p < 0.05. NCCLs were significantly associated with age groups (18-34, 35-44, 45-68 years. The unconditional logistic regression showed that the presence of NCCLs was better explained by age group (OR = 4.04; CI 95% 1.77-9.22 and occupational exposure to acid mists and chemical products (OR = 3.84; CI 95% 1.10-13.49, whereas the linear multiple regression revealed that NCCLs were better explained by years of smoking (p = 0.01 and age group (p = 0.04. The prevalence of NCCLs in the study population was particularly high (76.84%, and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  13. Long-Term Clinical Outcome after Treatment for High-Grade Cervical Lesions: A Retrospective Monoinstitutional Cohort Study

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    Annarosa Del Mistro

    2015-01-01

    Full Text Available Background. The aim of this retrospective observational study of women treated for cervical intraepithelial neoplasia grade 2 or worse (CIN2+ was to assess the long-term risk of residual/recurrent high-grade CIN. Materials and Methods. We evaluated 760 women treated by loop electrosurgical excision procedure (684 or conization (76 between 2000 and 2009, and followed up to June 30, 2014 (median follow-up 6.7 years, range 4–14. Visits every 6 months for the first year after treatment and yearly for up to the following 10 years included cytology, colposcopy when indicated, and HPV testing (search and typing. Results. CIN2+ or vaginal intraepithelial neoplasia grade 2 or worse (VAIN2+ was detected in 67 cases (8.8%, 39 at first follow-up and 28 after one/more negative visits. The risk of CIN2+ was higher in case of positive margins (odds ratio (OR 8.04, 95% CI 4.31–15.0, type 3 transformation zone (OR for CIN3 27.7, 95% CI 2.07–36.9, CIN3+ excision (OR 6.02, 95% CI 1.73–20.9, and positive high-risk HPV test at first follow-up (OR for HPV16: 20.6, 95% CI 6.8–62.6; OR for other hrHPV types: 18.3, 95% CI 5.9–57.0. Conclusion. Residual/recurrent high-grade CIN occurred in <9% cases, and the risk was associated with transformation zone type, lesion grade, margins status, and hrHPV test result at 6–12 months of follow-up.

  14. Non-carious cervical lesions (NCCLs) in a random sampling community population and the association of NCCLs with occlusive wear.

    Science.gov (United States)

    Yang, J; Cai, D; Wang, F; He, D; Ma, L; Jin, Y; Que, K

    2016-12-01

    This study investigated the prevalence, risk factors and association of occlusive wear with non-carious cervical lesions (NCCLs) in the general Chinese population. A total of 1320 subjects were recruited, and multistage and random sampling methods of survey spots were performed. All age groups comprised similar numbers of participants and equal numbers of males and females. Each subject completed a structured interview, and all teeth of each subject were examined by a practitioner to determine NCCLs and occlusive wear. Binary logistic regression was conducted by analysing the association of risk factors with the occurrence of NCCLs. Bivariate correlation analysis was performed by determining the association of NCCLs dimension or depth with the range of occlusive wear facets. Clinical assessment showed that the overall prevalence of subjects diagnosed with NCCLs was 63%. The proportion of subjects or teeth with NCCLs significantly increased with age. Pre-molars were the most commonly affected teeth. Single variables and interactive effects of variables associated with the occurrence of NCCLs include the following: age group, intensity of toothbrushing, frequency of fresh fruit consumption and interactive effect between intensity of toothbrushing and frequency of fresh fruit consumption. A weak positive correlation of the grading index was found between NCCLs dimension, size or depth and range of occlusive wear facets. This study reported the higher prevalence of NCCLs in the general Chinese population. Implementation of a combined strategy to reduce risk factors of NCCLs could be more effective than individual techniques; meanwhile, the occurrence of NCCL could be related to the wear degree of occlusive defects in the population studied.

  15. 宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用分析%Application Analysis the Cervical Scraping Cytology in Screening of Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张鸥

    2016-01-01

    目的:对宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用进行观察分析。方法选取我院2014年1月~2015年12月收治的214例进行宫颈癌筛查患者作为研究对象,进行宫颈刮片脱落细胞学检查,将宫颈刮片脱落细胞学检查结果同宫颈活组织病理检查相比较,观察分析宫颈刮片脱落细胞学检查在诊断宫颈癌、癌前病变的敏感度、特异度、阴性预测值和阳性预测值等。结果宫颈刮片脱落细胞学检查诊断宫颈癌、癌前病变敏感度为90.43%,特异度为89.17%,阳性预测值为86.73%,阴性预测值为92.24%,诊断准确率为89.72%。结论宫颈刮片脱落细胞学检查在筛查宫颈癌方面具有较高的敏感度。%Objective To observe and analyze the application of cervical scraping cytology in screening of cervical cancer.Methods Selected 214 cases of patients with cervical cancer screening from January 2014 to December 2015 in our hospital, for example, cervical scraping cytology, cervical scraping cytology results with cervical biopsy compared toobserve and analyze the cervical scraping off cytology in the diagnosis of cervical cancer and precancerous lesions of sensitivity, speciifcity, negative predictive and positive predictive value.Results The sensitivity of cervical cancer and precancerous lesion was 90.43%, the speciifcity was 89.17%, the positive predictive value was 86.73%, the negative predictive value was 92.24%, the diagnostic accuracy rate was 89.72%.Conclusion Cervical scraping cytology test in the screening of cervical cancer has a high sensitivity.

  16. From cancer screening to treatment: service delivery and referral in the National Breast and Cervical Cancer Early Detection Program.

    Science.gov (United States)

    Miller, Jacqueline W; Hanson, Vivien; Johnson, Gale D; Royalty, Janet E; Richardson, Lisa C

    2014-08-15

    The National Breast and Cervical Cancer Early Detection Program (NBCCEDP) provides breast and cervical cancer screening and diagnostic services to low-income and underserved women through a network of providers and health care organizations. Although the program serves women 40-64 years old for breast cancer screening and 21-64 years old for cervical cancer screening, the priority populations are women 50-64 years old for breast cancer and women who have never or rarely been screened for cervical cancer. From 1991 through 2011, the NBCCEDP provided screening and diagnostic services to more than 4.3 million women, diagnosing 54,276 breast cancers, 2554 cervical cancers, and 123,563 precancerous cervical lesions. A critical component of providing screening services is to ensure that all women with abnormal screening results receive appropriate and timely diagnostic evaluations. Case management is provided to assist women with overcoming barriers that would delay or prevent follow-up care. Women diagnosed with cancer receive treatment through the states' Breast and Cervical Cancer Treatment Programs (a special waiver for Medicaid) if they are eligible. The NBCCEDP has performance measures that serve as benchmarks to monitor the completeness and timeliness of care. More than 90% of the women receive complete diagnostic care and initiate treatment less than 30 days from the time of their diagnosis. Provision of effective screening and diagnostic services depends on effective program management, networks of providers throughout the community, and the use of evidence-based knowledge, procedures, and technologies.

  17. Chlamydia trachomatis among women with normal and abnormal cervical smears in Lagos, Nigeria

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    Maymunah Adeshola Adegbesan-Omilabu

    2014-06-01

    Results: The overall prevalence of C. trachomatis was 27.7% with a decreasing trend noted with age (P <0.05. The majority of women with C. trachomatis were in the reproductive age group of 25-45 years. 50% of women with abnormal smears were positive for C. trachomatis, compared to only 16.7% of the controls (X2 = 10.95; P = 0.001. There was no statistically significant association between prevalence of C. trachomatis and cervical cytological types (X2 = 1.892; P = 0.595 Conclusions: The study revealed an association between Chlamydia trachomatis and precancerous lesions of the cervix. Routine screening and treatment of sexually active adolescents and women in the reproductive age group is recommended as an indirect measure to reducing the incidence of cervical cancer in Nigeria. [Int J Reprod Contracept Obstet Gynecol 2014; 3(3.000: 501-506

  18. Overtreatment and undertreatment of cervical intraepithelial neoplasia%子宫颈上皮内瘤变治疗过度与不足得失分析

    Institute of Scientific and Technical Information of China (English)

    钱德英

    2011-01-01

    Cervical intraepithelial neoplasia (CIN) is precancerous cervical lesion. Reasonable intervention is one of primary measures for prevention and treatment of cervical cancer. In this paper, we investigated overtreatment and undertreatment of CIN in order to effectively block progression of CIN.%子宫颈上皮内瘤变是子宫颈癌的癌前病变,合理干预子宫颈上皮内瘤变是防治子宫颈癌的主要措施之一.文章就子宫颈上皮内瘤变干预过程出现的治疗过度与不足的原因和结果加以剖析,以期待有效阻断病变发展.

  19. Clinical application of immunohistochemical expression in differential diagnosis of breast cancer precancerous lesions and micro-invasive cancer%免疫组化表达在乳腺癌前驱病变与微浸润性癌鉴别诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    周晓燕; 胡玉莲; 王刚平; 王洪远; 王玲; 梅岚; 林慧

    2014-01-01

    目的:探讨分子标记物免疫表型在正常乳腺组织及癌前病变、浸润癌诊断和鉴别诊断中的应用。方法检测30例正常乳腺组织、20例非典型导管增生(ADH)、60例导管原位癌(DCIS)、20例小叶原位癌(LCIS)、90例浸润性癌(IDC)中 CK5/6、CK34βE12、P63、HHF-35、CK19、E-cadherin、P120-catenin 的表达,及其诊断和鉴别诊断价值。结果(1)30例正常乳腺组织定向干细胞 CK5/6阳性,CK19、HHF-35阴性;腺中间细胞表达 CK5/6和 CK19,腺终端细胞仅表达 CK19;肌中间细胞表达 CK5/6和 HHF-35,终端细胞仅表达 P63、HHF-35。(2)普通型导管增生(UDH)与 ADH 及低级别 DCIS 鉴别诊断:26例 UDH 导管周肌上皮 P63、HHF-35阳性,管内细胞 CK5/6、CK19、ER 阳性呈明显异质性,CK5/6、CK34βE12、P63、HHF-35呈马赛克样表达模式;ADH 不表达 CK34βE12和 CK5/6。(3)ADH 和低级别 DCIS 鉴别:17例 ADH、49例 DCIS 导管周肌上皮 CK5/6、CK34βE12、P63、HHF-35连续阳性,3例 ADH、11例部分导管肌上皮间断或连续阳性;管内细胞 CK5/6阴性,增生区内细胞 CK19弥漫阳性。(4)CK5/6、P63、HHF-35鉴别高级别 DCIS 及 IDC:高级别 DCIS 管周肌上皮 CK5/6、CK34βE12、P63、HHF-35阳性, IDC 则阴性。(5)导管原位癌小叶化与小叶原位癌:CK5/6、CK34BE12、E-caderin 及 P120组合可鉴别。结论免疫组化 CK5/6、34βE12、P63、HHF-35、CK19、E-cadherin 、P120-catenin 组合对乳腺癌前病变与早期微浸润癌具有辅助鉴别价值。%Objective Toinvestigatetheimmunophenotypicfeaturesofmolecularmarkersofbreast carcinoma and precancerous lesions. Methods The molecular markers staining modes of CK5/6、CK34βE12、P63、HHF-35、CK19、E-cadherin、P120-catenin in 30 cases normal breast tissues, 20 cases of ADH, 60 cases of DCIS, and 90 cases of carcinoma were observed. Results 30 cases of normal breast tissue stem cells only

  20. Papanicolaou test in the detection of high-grade cervical lesions: a re-evaluation based on cytohistologic non-correlation rates in 356 concurrently obtained samples.

    Science.gov (United States)

    Carns, Bhavini; Fadare, Oluwole

    2008-01-01

    Studies evaluating the routine Papanicolaou (Pap) test have traditionally used as the reference gold standard, the diagnoses on the follow-up histologic samples. Since the latter are typically obtained days to weeks after the Pap test, the accuracy of the resultant comparison may be affected by interim factors, such as regression of human papillomavirus, new lesion acquisitions or colposcopy-associated variability. A subset of our clinicians have routinely obtained cervical cytology samples immediately prior to their colposcopic procedures, which presented a unique opportunity to re-evaluate the test performance of liquid-based cervical cytology in detecting the most clinically significant lesions (i.e. cervical intraepithelial neoplasia 2 or worse: CIN2+), using as gold standard, diagnoses on cervical biopsies that were essentially obtained simultaneously. For each patient, cytohistologic non-correlation between the Pap test and biopsy was considered to be present when either modality displayed a high-grade squamous intraepithelial lesion (HGSIL)/CIN2+ while the other displayed a less severe lesion. Therefore, HGSIL/CIN2+ was present in both the Pap test and biopsy in true positives, and absent in both modalities in true negatives. In false positives, the Pap test showed HGSIL while the biopsy showed less than a CIN2+. In false negatives, Pap tests displaying less than a HGSIL were associated with biopsies displaying CIN2+. Combinations associated with "atypical" interpretations were excluded. A cytohistologic non-correlation was present in 17 (4.8%) of the 356 combinations reviewed. The non-correlation was attributed, by virtue of having the less severe interpretation, to the Pap test in all 17 cases. There were 17, 322, 0, and 17 true positives, true negatives, false positives and false negatives respectively. The sensitivity, specificity, positive predictive value and negative predictive value of the Pap test, at a diagnostic threshold of HGSIL, in identifying

  1. Combined periodontal and restorative approach to the treatment of gingival recessions with noncarious cervical lesions: a case treated with acellular dermal matrix allograft and compomer restorations.

    Science.gov (United States)

    Efeoğlu, Ahmet; Hanzade, Mete; Sari, Esra; Alpay, Hande; Karakaş, Ozan; Koray, Fatma

    2012-08-01

    Treatment of gingival recessions has become one of the most challenging procedures in periodontal plastic surgery. Various surgical options with predictable outcomes are available, but in cases with cervical lesions or restorations, optimal functional and esthetic results may require the combination of periodontal and restorative procedures. In this case report, one patient treated with acellular dermal matrix allograft and a coronally positioned flap in combination with compomer cervical restorations is presented. Clinical parameters were recorded immediately prior to surgery and after 12 months. Postoperatively, significant root coverage, reductions in probing depths, and gains in clinical attachment were observed. The final clinical results, esthetics, color match, and tissue contours were acceptable to both the patient and clinicians.

  2. Analysis of Sensitivity, Specificity, and Positive and Negative Predictive Values of Smear and Colposcopy in Diagnosis of Premalignant and Malignant Cervical Lesions.

    Science.gov (United States)

    Barut, Mert Ulaş; Kale, Ahmet; Kuyumcuoğlu, Umur; Bozkurt, Murat; Ağaçayak, Elif; Özekinci, Server; Gül, Talip

    2015-12-10

    BACKGROUND This study aimed to examine the positive and negative predictive value in the diagnosis of premalignant and malignant lesions of cervical colposcopy, the sensitivity and specificity of smear, and to evaluate the correlation with histopathology of abnormal cytology and colposcopy. MATERIAL AND METHODS The criteria for inclusion of patients with unhealthy cervix in the study were: Erosion, Chronic cervicitis, and Healed lacerations, Hypertrophied cervix, bleeding on touch, suspicious growth/ulcer/polyp on the cervix, and abnormal discharges from the cervix. Women with frank carcinoma cervix, pregnant females, patients with bleeding per vaginum at the time of examination, and those who had used vaginal medications, vaginal contraceptives or douches in the last 48 h of examination were excluded from the study. Demographic analysis was performed for 450 patients who were admitted to the clinic. Sensitivity, specificity, positive predictive value and negative predictive values of patients to identify cervical pathologies of smear and colposcopy were histopathologically calculated. The statistical software package SPSS 15.0 (SPSS Inc., Chicago, IL, USA) and Spearman's and Chi-Square tests were used for statistical analysis. RESULTS Sensitivity, specificity, PPD and NDP of smear were 0.57%, 0.76%, 0.26%, 0.92% respectively. Sensitivity, specificity, PPD and NDP of colposcopy were 0.92%, 0.67%, 0.52%, 0.96% respectively. A statistically significant correlation was found between abnormal cytology and histopathology, and abnormal colposcopy finding and histopathology. CONCLUSIONS Women with clinical diagnosis of unhealthy cervix should be evaluated by cytology to detect any premalignant or malignant lesions. It was concluded that Pap smear, colposcopy and histopathology should be collectively evaluated to evaluate cervical findings in low socio-economic regions.

  3. Frequency of Human Papillumavirus among Women with High-Grade Squamous Intraepithelial Lesions and Invasive Cervical Cancer Attending Shahid Beheshti University of Medical Sciences Clinics, Tehran, Iran.

    Directory of Open Access Journals (Sweden)

    Nahid Khodakarami

    2014-11-01

    Full Text Available The previous studies reported some information about prevalence release of high-risk HPV types in HSIL or cervical cancer globally and in Iran, however, this information is not enough for final judgment about vaccination against HPV or any screening program. The aim of the present study was to assess the HPV type distribution in HSIL and ICC specimens of women attending Shahid Beheshti University of Medical Sciences teaching hospitals, Tehran, Iran for treatment during 10 years.This retrospective- descriptive study evaluated the HPV type distribution of pathologic specimens of Iranian women with invasive cervical cancer (ICC and high-grade squamous cell intraepithelial lesions (HSIL. Formalin-fixed tumor biopsies that were retrieved from women presenting with histological confirmation for ICC and 17 pathologic confirmation for HSIL specimens.The most frequently identified HPV type 16 among both groups, women with invasive cervical cancer (4-2.18% and women with High Grade Squamous Intraepithelial Lesion (29.41%, followed by HPV18, HPV31 and 26. HPV16 and / or 18 accounted for 82.2% of all infected samples.The dominance of HPV16 over other high-risk types might be even higher than in a region with low HPV exposure. However, there was no strong evidence for any judgment that show to the policy makers; which one is cost-effectiveness and feasibility for cervical cancer prevention in Iran, vaccination, screening or both? More population based study and national meta-analysis needed for better understanding of HPV prevalence and HPV DNA patterns in Iran.

  4. 宫颈细胞学涂片联合阴道镜对宫颈病变患者的诊断效果%The Effect of Cervical Smear Combined with Colposcope in the Diagnosis of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    王芳; 李卫民; 秦迎辉; 卢文华

    2016-01-01

    Objective:To analyze the effect of cervical smear combined with colposcope in the diagnosis of cervical lesions.Method:In our hospital for cervical smears 204 patients were selected,they were given cervical smears and colposcopy,the clinical results were analyzed.Result:204 patients, 93 cases were abnormal,the meaning of unknown atypical squamous cell and gland(ASCUS) 56 cases,low-grade squamous intraepithelial lesions(LSIL) 22 cases,highly squamous intraepithelial lesion(HSIL) 14 cases,1 case of squamous cell carcinomas(SCC).Colposcope examination was performed,the sensitivity was 89.4%(59/66),specific degree was 77.0%(107/139),false negative rate was 10.6%(7/66),the false positive rate was 23.0%(32/139),after LEEP confirmed diagnosis and misdiagnosis and deficiencies.In cervical cytological smear combined colposcope examination,the sensitivity was 94.7%(54/57),43.5%(47/85), false negative rate was 5.2%(3/57),false positive rate was 56.5%(48/85).LEEP confirmed the accuracy was 90.5%.Conclusion:Cervical smear combined colposcope examination can not only reduce missed diagnosis and misdiagnosis rate,also can be a effective method for treatment of cervical lesion.%目的:采用宫颈细胞学涂片联合阴道镜对宫颈病变患者进行诊断分析.方法:选取在笔者所在医院进行宫颈涂片检查的204例患者,对其分别进行宫颈细胞学涂片检查、阴道镜,分析临床结果.结果:204例患者中,93例异常者,其中意义不明的不典型鳞状细胞和腺细胞(ASCUS)56例,低度鳞状上皮内病变(LSIL)22例,高度鳞状上皮内病变(HSIL)14例,鳞癌(SCC)1例.对患者进行阴道镜检查,患者灵敏度为89.4%(59/66),特异度为77.0%(107/139),假阴性率为10.6%(7/66),假阳性率为23.0%(32/139).经LEEP证实后,存在漏诊和诊断不足情况.宫颈细胞学涂片联合阴道镜检查,灵敏度为94.7%(54/57),特异度为43.5%(47/85),假阴性率5.2%(3/57),假阳性率为56.5%(48/85).经LEEP证实后准确率为90.5%.结

  5. 宫颈病变筛查策略的效果分析%The Evaluation of the Effect of Cervical Lesions Screening Strategies

    Institute of Scientific and Technical Information of China (English)

    龙腾飞

    2011-01-01

    Cervical cancer is one of the most common female malignant cancers , with an increasing incidence and earlier onset ages. In developed countries, the systematic screening programmes have reduced the morbidity and mortality resulting from cervical cancer. The screening program is the main means to prevent and control cervical cancer. The main screening tools are visual inspection, cytology, human papillomavirus testing and colposcopy. The scholars all over the world made great efforts to seek appropriate cervical cancer screening solutions in either single or comhines use of various methods. Here is to review on the effert of cervical lesions screening strategies in recent years inside and outside China.%宫颈癌是妇科常见的恶性肿瘤之一,其发病率逐年升高,且发病年龄有年轻化的趋势.对宫颈癌的筛查是预防和控制宫颈癌的主要手段,也是极大限度防治宫颈癌的最好途径.宫颈癌筛查方法包括肉眼观察、宫颈细胞学、人类乳头瘤病毒检测及阴道镜检查等.世界各地学者做了许多努力来寻求适宜的宫颈癌筛查方案,包括各种方法的单独及联合使用.现就近年来国内外宫颈病变筛查策略的效果予以综述.

  6. 电子染色内镜对早期胃癌及癌前病变边界的评估价值%The Assessment Value of Electronic Staining Endoscopy for Early Gastric Cancer and Precancerous Lesions Border

    Institute of Scientific and Technical Information of China (English)

    夏阳

    2014-01-01

    Objective Discuss the assessment value of electronic staining endoscopy for early gastric cancer and precan -cerous lesions border .Methods 212 patients with gastroscope inspection were selected ,patients were divided into group A ( e-lectron staining endoscopy group ) and group B (normal endoscopy group ),each 106 cases.compared two groups of gastroscope inspection result .Results Electron staining endoscopy and common endoscopic detection of atrophic gastritis and intestinal meta -plasia was no statistically difference in diagnosis accuracy rate ,P>0.05.Electron staining endoscopy diagnosis of atypical hyper-plasia and early gastric cancer was significantly higher than that of ordinary endoscopy ,P<0.05.Conclusion Using electronic dyeing endoscopic biopsy under the guidance can enhance the detection rate of dysplasia and early gastric cancer .%目的:探讨电子染色内镜对早期胃癌及癌前病变边界的评估价值。方法选择在我院进行胃镜检查的患者共212例,根据检查方法不同分为A组(电子染色内镜组)及B组(普通内镜组),每组106例,对比2组胃镜检测效果。结果电子染色内镜与普通内镜检测萎缩性胃炎及肠上皮化生的诊断准确率差异无统计学意义,P>0.05。电子染色内镜检测不典型增生和早期胃癌的准确率显著高于普通内镜,P<0.05。结论在电子染色内镜指导下进行活检,可以提高不典型增生和早期胃癌的检出率。

  7. Evaluation of the p16 and Ki-67 Biomarkers as Predictors of the Recurrence of Premalignant Cervical Cancer Lesions after LEEP Conization.

    Science.gov (United States)

    Leite, Paulo Macêdo de Oliveira; Tafuri, Luciene; Costa, Maria Zélia de Oliveira; Lima, Maria Inês de Miranda; Simões, Renata Toscano

    2017-02-23

    Objective To evaluate the expressions of biomarkers p16 and Ki-67 in low-grade (LG) or high-grade (HG) lesions, and to relate them to risk factors and the recurrence of these lesions. Methods A retrospective case-control study of 86 patients with LG and HG lesions who underwent a loop electrosurgical excision procedure (LEEP) between 1999 and 2004. The control group was composed of 69 women with no recurrence, and the study group, of 17 patients with recurrence. All patients were followed-up over a two-year period after surgery, and screened every six months, including cytology and colposcopy. Biopsy samples collected from LEEP were submitted to immunohistochemical analysis for p16 and Ki-67. The statistical analysis was performed using the Statistical Package for the Social Sciences software (SPSS, IBM-SPSS, Inc., Chicago, IL, US), with a significant p ​​p16 and Ki-67, separately or combined, showed no relation to recurrence on the total analysis. However, evaluating specifically HG lesions, the positive expression (2+ and 3+) of p16/Ki-67 was associated with recurrence (0.010). In addition, p16 isolated was also more expressive in HG lesions (2+ and 3 + , p = 0.018), but it was unrelated to recurrence. Conclusion Proteins p16 and Ki-67, both isolated and combined, are not reliable primary markers for the recurrence of cervical lesions in the majority of LG lesions. However, analyzing only the group with prior diagnosis of HG lesions, the expressions of p16 and of p16/Ki-67 were associated with recurrence, and they may be useful in monitoring these cases.

  8. Papanicolau smear chances to be diagnostic for cervical squamous intraepithelial lesions (SIL) with or without detectable HPV DNA at in situ hybridization analysis.

    Science.gov (United States)

    Sopracordevole, F; Cadorin, L; Muffato, G; De Benetti, L; Parin, A

    1993-01-01

    The Authors have correlated 39 cervical diagnostic biopsies for squamous intraepithelial lesions (SILs) with correspective Papanicolau smears (PS), with relation to the presence or the absence of HPV of oncogenic type (HPV-one) detected by in situ hybridization (ISH). Agreement between cytological and histological diagnosis was present in 14 of 16 cases with detectable HPV-one and only in 12 of 23 cases without detectable HPV-one at ISH. The importance of the HPV type in the SILs with relation to the diagnostic accuracy of Papanicolaou smears has been discussed.

  9. Comparison of Onclarity Human Papillomavirus (HPV) Assay with Hybrid Capture II HPV DNA Assay for Detection of Cervical Intraepithelial Neoplasia Grade 2 and 3 Lesions

    DEFF Research Database (Denmark)

    Bottari, F; Sideri, M; Gulmini, C;

    2015-01-01

    , to concurrent cytology and histology results, in order to evaluate its performance in detecting high-grade cervical lesions. A population of 567 women, including 325 with ≥ASCUS (where ASCUS stands for atypical cells of undetermined significance) and any HC2 result and 242 with both negative cytology...... that the BD Onclarity HPV assay has sensitivity comparable to that of the HC2 assay, with a trend to an increased specificity. Moreover, as Onclarity gives the chance to discriminate between the different genotypes, we calculated the genotype prevalence and the absolute risk of CIN2+: HPV 16 was the most...

  10. 幽门螺杆菌感染在喉癌及癌前病变中与COX-2表达的相关研究%The relevant research of helicobacter pylori infection in laryngeal cancer and precancerous lesions and cox-2 expression

    Institute of Scientific and Technical Information of China (English)

    杨艳青; 张丽萍; 薛振伟; 王立坤; 刁玉华

    2014-01-01

    目的:检测喉癌及癌前病变组织中的HP(幽门螺杆菌)感染及环氧化酶-2(coX-2)表达,为寻找更有效的喉癌预防措施提供理论依据。方法免疫组化法和硼酸亚甲蓝法分别检测50例喉单纯增生、喉癌前病变、喉癌中HP感染情况和coX-2表达。结果喉单纯性增生、喉癌前病变、喉癌HP感染阳性率分别为:32%、66%、24%,P<0.05;coX-2表达阳性率分别为:18%、46%、80%,P<0.05;HP阳性组coX-2表达均高于HP阴性组,其中,HP阳性组喉癌前病变coX-2表达高于HP阴性组,P<0.05。结论1、HP感染可能是喉癌发生的一个早期事件;将为喉癌前期病变的干预性治疗提供一个临床的参考指标。2、coX-2的表达与喉癌的发生、发展可能有关。3、HP感染后诱导喉癌前病变中coX-2的表达,可能是HP致癌机制之一。%Objective By detecting the relationship between helicobacter pylori(HP) infection and the expression of cox-2 in laryngeal cancer and precancerous tissue,as to provide theoretical basis for looking for effective laryngeal cancer prevention measures. Methods the specimens from 50 cases of laryngeal simple hyperplastic , lesion before larynx cancer and laryngeal cancer ,were detected the diversity of positive expression rates of cox-2 by immunohistochemical method and detection of HP infection by improved borate methylene blue method. Result in throat simple hyperplastic,lesion before larynx cancer and laryngeal cancer:the positive rate of HP infection is respectively:32%,66%, 24%,P<0.05;the positive rate of cox-2 is respectively:18%,46%80%,P<0.05;Positive expression of cox-2 in HP positive group were higher than in HP negative group,among them,positive expression of cox-2 in HP positive group were higher than in HP negative group in the lesion before larynx cancer,P<0.05.Conclusion 1, HP infection may be an early event of laryngeal cancer and provide a clinical reference index with the

  11. New paradigms in cervical cancer prevention: opportunities and risks

    Directory of Open Access Journals (Sweden)

    Giorgi Rossi Paolo

    2008-12-01

    Full Text Available Testing for the DNA of high-risk types of papilloma virus (HPV is more sensitive than cytology in detecting pre-cancerous lesions. One of the main advantages will be the possibility of applying prolonged screening intervals. However adequate screening protocols (age of start and stop, screening intervals, management of HPV positive women need to be applied in order to avoid over-referral to colposcopy and over-treatment and to maintain sustainable costs. Further follow-up of running trials and research on molecular markers will better define these parameters. The new situation will require organised screening programmes with rigorous protocols and monitoring. This will be even more needed when women vaccinated for HPV 16 and 18 will be screened. Research on how to best screen vaccinated women is a priority. This paper proposes an overview of the plausible impact of new technologies in cervical cancer screening in the near future and in the vaccinated cohorts.

  12. 宫颈液基细胞学联合HPV检查在宫颈病变筛查中的评价%Evaluation of Liquid-based Cervical Cytology and HPV Examination in the Screening of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    高洁; 方法; 战英杰

    2016-01-01

    Objective To research the application value of HPV combined with TCT in the screening of cervical lesions. Methods 424 cases of female patients diagnosed in the gynecology clinic of our hospital from January 2014 to December 2014 were selected as the research objects and were given HPV and TCT detection and pathologic biopsy vaginoscopy, and the methodology of screening of cervical lesions was evaluated through the single detection of cervical HPV and TCT and combined detection of the two using the pathological results as “gold standard”. Results Of 424 cases of female patients, 49.5% was HPV positive, and with the continuous progress of the degree of cervical disease, HPV positive rate also showed an increasing trend, TCT examination results of 424 cases: the positive rate of above-ASCUS lesions was 49.3%, ASC oc-curred to 105 cases, low-grade squamous intraepithelial lesion occurred to 69 cases, high-grade squamous intraepithelial lesion occurred to 34 cases, squamous-cell carcinoma occurred to 1 case, compared with those of the single examination, the sensitivity, Youden index and negative predictive value of the combined examination in the screening of above-CINI-were improved in varying degrees. Conclusion TCT combined with HPV detection is of an important clinical value to the screening of cervical lesions.%目的:研究人乳头瘤病毒(HPV)联合宫颈液基薄层细胞学(TCT)检测在宫颈病变筛检中的应用价值。方法整群选取2014年1—12月以424例妇科门诊就诊女性为研究对象,分别行HPV、TCT检测及阴道镜下病理活检,以病理学结果为“金标准”,对宫颈HPV及TCT的单独检测,以及二者联合检测对宫颈病变筛查的方法学评价。结果424例就诊女性患者49.5%为HPV阳性,且随着宫颈疾病病变程度不断进展,HPV阳性率也呈现升高趋势;424例TCT检查结果:宫颈不典型鳞状细胞(ASCUS)以上病变阳性率为49.3%,其中ASC105例,

  13. Human papillomavirus (HPV detected in restored plasma DNA from women diagnosed with pre-invasive lesions and invasive cervical cancer

    Directory of Open Access Journals (Sweden)

    Yazmín Rocío Arias

    2010-06-01

    Full Text Available Objective: To improve the sensitivity of Human Papillomavirus (HPV detection in plasma from high-grade cervical neoplasia patients (CIN III and cervical cancer (CC evaluating any likely correlation with disease stage. Method: We subjected plasma DNA isolates from 112 patients (CIN and ICC to a pre-PCR restoration treatment to improve detection sensitivity. HPV-specific sequences were detected by conventional PCR both in cervical scrapes and plasma DNA obtained from each patient. For every single DNA sample, both non-restored and restored isolates were PCR analyzed. Results: We detected HPV in plasma DNA isolates with significantly higher efficiency on restored plasma-DNA as compared to each non-restored equivalent, still maintaining close correlation with the clinical stage of the cases. By analyzing plasma-DNA isolates we could classify as HPV positive >50.0% of the cases that were previously known to be positive from the cervical scrape based assay. Interestingly, 100% of the cases in which subtype HPV18 was detected in cervical scrapes were also positive in plasma DNA. Conclusions: Restoration of plasma DNA from cervical cancer patients allows a more sensitive PCR-based HPV detection, maintaining the correlation to disease stage traditionally observed.

  14. Human papillomavirus (HPV detected in restored plasma DNA from women diagnosed with pre-invasive lesions and invasive cervical cancer

    Directory of Open Access Journals (Sweden)

    Edward Fabián Carrillo

    2010-06-01

    Full Text Available Objective: To improve the sensitivity of Human Papillomavirus (HPV detection in plasma from high-grade cervical neoplasia patients (CIN III and cervical cancer (CC evaluating any likely correlation with disease stage.Method: We subjected plasma DNA isolates from 112 patients (CIN and ICC to a pre-PCR restoration treatment to improve detection sensitivity. HPV-specific sequences were detected by conventional PCR both in cervical scrapes and plasma DNA obtained from each patient. For every single DNA sample, both non-restored and restored isolates were PCR analyzed.Results: We detected HPV in plasma DNA isolates with significantly higher efficiency on restored plasma-DNA as compared to each non-restored equivalent, still maintaining close correlation with the clinical stage of the cases. By analyzing plasma-DNA isolates we could classify as HPV positive >50.0% of the cases that were previously known to be positive from the cervical scrape based assay. Interestingly, 100% of the cases in which subtype HPV18 was detected in cervical scrapes were also positive in plasma DNA.Conclusions: Restoration of plasma DNA from cervical cancer patients allows a more sensitive PCR-based HPV detection, maintaining the correlation to disease stage traditionally observed.

  15. Different expressions of Wnt5a in pancreatic cancer and precancerous lesions%Wnt5a在胰腺癌及癌前病变中的表达差异分析

    Institute of Scientific and Technical Information of China (English)

    王伟; 满晓华; 郑建明; 李兆申; 高军; 龚燕芳

    2012-01-01

    Objective To observe the expression of Wnt5a protein in different pancreatic tissues and to evaluate the role of Wnt5a in the carcinogenesis of pancreatic ductal adenocarcinoma ( PDAC), so as to provide new clues for the diagnosis and treatment of pancreatic cancer. Methods Immunohistochemical SP method was used to examine the expressions of Wnt5a protein in 21 normal pancreatic duct tissues, 73 pancreatic intraepithelial neoplasia(PanIN)-l tissues,29 PanIN-2,16 PanIN-3 tissues,20 intraductal papillary mucinous neoplasm (IPMN) adenoma (IPMA) tissues, 13 IPMN-borderline (IPMB) tissues, 19 IPMN-carcinoma (IPMC) tissues and 50 PDAC tissues. The correlation of Wnt5a expression with clinicopathologic characteristics and postoperative survival of PDAC patients was analyzed. Results Wnt5a expression increased with the progression of the lesions in the following order, NP(0)→PanIN-l(l. 90±l. 192)→PanIN-2(3. 03±l. 322)→PanIN-3(4. 88± 1.455) orNP(O)→IPMAQ. 40±0.940)→IPMB (2. 62±1.502) → IPMC (3.00±1.374),and PDACC3. 11±2. 635). Wnt5a expression was significantly correlated with tumor proliferation activity, distant metastasis, TNM staging and postoperative survival (P<0. 05). The median survival periods of PDAC patients with high and low Wnt5 expression were 15 months and 21 months,respectively(P=O. 015). Conclusion Wnt5a is involved in the development and progression of PDAC. Enhancement of Wnt5a expression is an early event in PDAC) sustained or over-expression of Wnt5a may affect the proliferation, invasion and metastases of pancreatic cancer cells, and may indicate a poor prognosis.%目的 检测Wnt5a基因在不同胰腺组织中的蛋白表达水平,探讨其在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)发生中的作用,为胰腺癌的早期诊疗提供新线索.方法 免疫组化SP法检测Wnt5a在21灶正常胰腺导管(normal pancreatic duct,NP)、73灶胰腺上皮内瘤变(pancreatic intraepithelial neoplasia,PanIN)-1、29

  16. CERVICAL ACID PHOSPHATASE: EVALUATION AS AN ADJUVANT TO PAPANICOLAOU SMEAR SCREENING IN CERVICAL CANCER DETECTION

    Directory of Open Access Journals (Sweden)

    Niranjan

    2015-02-01

    Full Text Available INTRODUCTION: Carcinoma of cervix accounts for 15% of all cancers diagnosed worldwide and is the second most common cancer in women. In the year 2000 there were over 4,71,000 new cases diagnosed and 2,88,000 deaths from cervical cancer. (1 Approximately 79% of these deaths occurred in developing countries. (2 Cervical cancer is preventable, but most women in poorer countries do not have access to effective screening programs. In India it is estimated that approximately 100,000 women develop cervical cancer each year. (3 Cancer cervix occupies either the top r ank or second among cancers in women in developing countries, whereas, in the developed countries cancer cervix does not find a place even in top five leading cancers in women. This is due to routine screening by cervical smear. Cervical smear cytology scr eening by Papanicolaou (Pap stained smears is the most efficacious and cost - effective method of cancer screening, decreasing the incidence and mortality from cervical cancer. (4 However, cervical smear screening has significant rates of false - positive and false - negative results, ranging from 10.3% for false positive cases to 5.6% for false negative cases. (5,6 To improve the detection and screening of cancerous and precancerous lesions of the cervix a number of sophisticated tests are available which are e xpensive and can be done only in a tertiary laboratory. To over - come this problems a cost effective cytochemical stain was introduced to measure the acid phosphatase activity in the cervical epithelium. (7 Since the description of the new Cervical Acid Phosphatase Test (CAP Test for visualization of cervical acid phosphatase activity (CAP inside abnormal cervical cells on smears, it has become possible to explore this enzyme as a biomarker for cervical dys plasia, and as a possible surrogate for PAP smear in detection of cervical intraepithelial neoplasia (CIN. AIMS AND OBJECTIVES: To assess the utility of Cervical Acid

  17. Detection of Cervical Cancer and High Grade Neoplastic Lesions by a Combination of Liquid‐Based Sampling Preparation and DNA Measurements Using Automated Image Cytometry

    Directory of Open Access Journals (Sweden)

    Xiao Rong Sun

    2005-01-01

    Full Text Available Objective: To establish if measurements of DNA ploidy could be used to assist cytopathologists and cytotechnologists in population based cervical cancer screening programs in countries where manually reading the slides is impossible due to the lack of sufficient skilled cytotechnologists. The goal of such program is to identify only clinically significant lesions, i.e. those where a clinical intervention to remove the lesion is required immediately. Study Design: A total of 9905 women were enrolled in the study. Cervical samples were taken with a cervix brush that was then placed into a fixative solution. The cells were separated from mucus by mechanical and chemical treatment and then deposited onto microscope slides by a cytocentrifuge. Two slides were prepared from each case; one slide was stained by Papanicolaou stain for manual cytology examination, while the other slide was stained by a DNA specific stain. The latter slide was used to determine the relative amount of DNA in the cell nuclei. Results: A total of 876 women were followed by colposcopy examination where biopsies were taken from the visible lesions or from suspicious areas and histopathology diagnosed 459 as normal or benign cases, 325 as CIN1, 36 as CIN2, 25 as CIN3/CIS, and 31 as invasive cancer. Of these 876 cases, manual cytology called 655 normal or ASCUS, 197 as LSIL, 16 cases as HSIL, and 8 as cancer. DNA measurements found 704 cases having no cells with DNA greater than 5c, 98 cases where there were 1 or 2 cells having DNA amount greater than 5c, and 74 cases where there were 3 or more cells having DNA amount greater than 5c. If manual cytology were to be used to refer all cases of HSIL and cancer to colposcopy and biopsy, 23 lesions that had to be removed would have been discovered (2 CIN2, 11 CIN3/CIS, and 10 cancers, for a sensitivity of 25.0±5.2% at specificity of 99.9±0.1%. If DNA assisted cytology were to be used instead, and all cases having 3 or more cells with

  18. Age trends in the prevalence of cervical squamous intraepithelial lesions among HIV-positive women in Cameroon: a cross-sectional study

    LENUS (Irish Health Repository)

    Atashili, Julius

    2012-10-29

    AbstractBackgroundCervical squamous intra-epithelial lesions (SIL) are more frequent in HIV-positive women overall. However the appropriate age at which to begin and end cervical cancer screening for early detection of lesions in HIV-positive women is not clear. We assessed the age-specific prevalence of any SIL and SIL requiring colposcopy in HIV-positive women in Cameroon.MethodsWe enrolled, interviewed and conducted conventional cervical cytology in 282 women, aged 19--68 years, initiating antiretroviral therapy in three clinics in Cameroon. In bivariable analyses, the crude relationship between age and the presence of lesions was assessed using locally weighted regression (LOWESS) methods. In multivariate analyses, generalized linear models with prevalence as the outcome, an identity link and a binomial distribution, were used to estimate prevalence differences. Bias analyses were conducted to assess the potential effect of inaccuracies in cytology.ResultsSIL were detected in 43.5% of the 276 women with satisfactory samples, 17.8% of whom had ASC-H\\/HSIL. On average, women aged 26 to 59 tended to have a slightly higher prevalence of any SIL than other women (Prevalence difference PD: 6.5%; 95%CI: -11.4, 24.4%). This PD was a function of CD4 count (heterogeneity test p-value =0.09): amongst patients with CD4 counts less than 200cells\\/uL, the prevalence was higher in patients aged 26--59, while there was essentially no difference amongst women with CD4 counts greater than 200 cells\\/uL. ASC-H\\/HSIL were present in women as young as 19 and as old as 62. Overall the prevalence of ASC-H\\/HSIL increased by 0.7% (95%CI: -3.8%, 5.1%) per decade increase in age.ConclusionBoth severe and less severe lesions were prevalent at all ages suggesting little utility of age-targeted screening among HIV-positive women. Nevertheless, the long-term evolution of these lesions needs to be assessed in prospective studies.

  19. Clinical interest of postural and vestibulo-ocular reflex changes induced by cervical muscles and skull vibration in compensated unilateral vestibular lesion patients.

    Science.gov (United States)

    Dumas, Georges; Lion, Alexis; Gauchard, Gérome C; Herpin, Guillaume; Magnusson, Måns; Perrin, Philippe P

    2013-01-01

    Skull vibration induces nystagmus in unilateral vestibular lesion (UVL) patients. Vibration of skull, posterior cervical muscles or inferior limb muscles alters posture in recent UVL patients. This study aimed to investigate the postural effect of vibration in chronic compensated UVL patients. Vibration was applied successively to vertex, each mastoid, each side of posterior cervical muscles and of triceps surae in 12 UVL patients and 9 healthy subjects. Eye movements were recorded with videonystagmography. Postural control was evaluated in eyes open (EO) and eyes closed (EC) conditions. Sway area, sway path, anteroposterior and medio-lateral sways were recorded.A vibration induced nystagmus (VIN) beating toward the healthy side was obtained for each UVL patient during mastoid vibration. In EO, only sway path was higher in UVL group during vibration of mastoids and posterior cervical muscles.The EO postural impairments of UVL patients could be related to the eye movements or VIN, leading to visual perturbations, or to a proprioceptive error signal, providing an erroneous representation of head position. The vibration-induced sway was too small to be clinically useful. Vestibulo-ocular reflex observed with videonystagmography during mastoid vibration seems more relevant to reveal chronic UVL than vestibulo-spinal reflex observed with posturography.

  20. Differentiating between endocervical glandular neoplasia and high grade squamous intraepithelial lesions in endocervical crypts: cytological features in ThinPrep and SurePath cervical cytology samples.

    Science.gov (United States)

    Thiryayi, Sakinah A; Marshall, Janet; Rana, Durgesh N

    2009-05-01

    A recent audit at our institution revealed a higher number of cases diagnosed as endocervical glandular neoplasia on ThinPrep (TP) cervical cytology samples (9 cases) as opposed to SurePath (SP) (1 case), which on histology showed only high-grade cervical intraepithelial neoplasia (CIN) with endocervical crypt involvement (CI). We attempted to ascertain the reasons for this finding by reviewing the available slides of these cases, as well as slides of cases diagnosed as glandular neoplasia on cytology and histology; cases diagnosed as high-grade squamous intraepithelial lesions (HSIL) on cytology which had CIN with CI on histology and cases with mixed glandular and squamous abnormalities diagnosed both cytologically and histologically. Single neoplastic glandular cells and short pseudostratified strips were more prevalent in SP than TP with the cell clusters in glandular neoplasia 3-4 cells thick, in contrast to the dense crowded centre of cell groups in HSIL with CI. The cells at the periphery of groups can be misleading. Cases with HSIL and glandular neoplasia have a combination of the features of each entity in isolation. The diagnosis of glandular neoplasia remains challenging and conversion from conventional to liquid based cervical cytology requires a period of learning and adaptation, which can be facilitated by local audit and review of the cytology slides in cases with a cytology-histology mismatch.

  1. Annual national direct and indirect cost estimates of the prevention and treatment of cervical cancer in Brazil

    Science.gov (United States)

    Novaes, Hillegonda Maria Dutilh; Itria, Alexander; Silva, Gulnar Azevedo e; Sartori, Ana Marli Christovam; Rama, Cristina Helena; de Soárez, Patrícia Coelho

    2015-01-01

    OBJECTIVE: To estimate the annual direct and indirect costs of the prevention and treatment of cervical cancer in Brazil. METHODS: This cost description study used a "gross-costing" methodology and adopted the health system and societal perspectives. The estimates were grouped into sets of procedures performed in phases of cervical cancer care: the screening, diagnosis and treatment of precancerous lesions and the treatment of cervical cancer. The costs were estimated for the public and private health systems, using data from national health information systems, population surveys, and literature reviews. The cost estimates are presented in 2006 USD. RESULTS: From the societal perspective, the estimated total costs of the prevention and treatment of cervical cancer amounted to USD $1,321,683,034, which was categorized as follows: procedures (USD $213,199,490), visits (USD $325,509,842), transportation (USD $106,521,537) and productivity losses (USD $676,452,166). Indirect costs represented 51% of the total costs, followed by direct medical costs (visits and procedures) at 41% and direct non-medical costs (transportation) at 8%. The public system represented 46% of the total costs, and the private system represented 54%. CONCLUSION: Our national cost estimates of cervical cancer prevention and treatment, indicating the economic importance of cervical cancer screening and care, will be useful in monitoring the effect of the HPV vaccine introduction and are of interest in research and health care management. PMID:26017797

  2. Clinical Value of LCT and HR-HPV in the Diagnosis of Cervical Lesions%LCT及HR-HPV诊断宫颈病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    李玉环; 戴红英; 闫丽萍; 丁朝霞

    2015-01-01

    Objective To study liquid-based cervical cytology and high risk human papilloma virus testing and bi⁃opsy in the diagnosis, discusses the cervical liquid based cytology and high-risk human papilloma virus detection value to the diagnosis of cervical lesions. Methods Retrospective analysis of the Affiliated Hospital of Qingdao University outpa⁃tient 20479 cases of LCT screening from January 2013 to January 2014. LCT positives were further investigated by HR-HPV testing and colposcopy cervical biopsy pathology, compare the accuracy of the LCT positive results and HR-HPV test results with cervical biopsy and colposcopy results with pathology detection results as the gold standard of diagnosis. Results 1)The consistence rates of ASCUS, LSIL, ASCH, HSIL by LCT and colposcopic multiple biopsies were 54.55%, 62.65%, 88.24%, 92.99%. HR- HPV infection rates of ASCUS, LSIL, ASCH and HSIL respectively were 70.00%, 88.71%, 84.91%, 89.66%. There was seriously statistical significance(P<0.001).2)HR-HPV positive and nega⁃tive groups compared with the high-level cervical lesion detection rates were 48.14%, 17.39%(P<0.001). There was se⁃riously statistical significance. Conclusion Liquid-based cytology and high-risk human papillomavirus detection is an effective screening method for detecting cervical lesions, higher diagnostic accuracy of both joint detection. High-risk hu⁃man papillomavirus detection for cytologic diagnosis of atypical squamous cells of patients has significant shunt value.%目的:探讨LCT和HR-HPV对宫颈病变的诊断价值。方法对20479例患者行LCT检查,LCT阳性者行HR-HPV检测及电子阴道镜下宫颈活检组织病理检查,以组织病理学结果作为诊断的金标准,比较LCT阳性结果、HR-HPV检测结果与阴道镜下宫颈活检病理结果的符合率。结果1)LCT结果为ASCUS、LSIL、ASCH、HSIL与阴道镜下宫颈活检病理结果的符合率分别为54.55%、62.65%)、88.24%、92.99%

  3. Etiology and Prevention of Cervical Cancer%宫颈癌病因及预防

    Institute of Scientific and Technical Information of China (English)

    杨红杰; 李忻琳

    2011-01-01

    This article discussed the cause and prevention of cervical cancer from a philosophical point of view. Cervical cancer is the combined result of internal reason and external reason. Experimental and clinical studies found that high- risk HPV (one of human papillomavirus family) infection is the ongoing occurrence of cervical cancer and precancerous lesions, it's the main external factor. This article will discuss the HPV genotyping as an important elemen of cervical cancer screening system in the prevention of cervical cancer.%从哲学角度讨论宫颈癌的病因,思考临床工作中如何预防宫颈癌.宫颈癌的发生是内因外因综合作用的结果.实验与临床研究发现人类乳头瘤病毒(HPV)家族中的高危型HPV的持续感染是宫颈癌及其癌前痛变发生的主要外因.本文将讨论HPV分型检测作为宫颈癌筛查系统的一个重要元素在预防宫颈癌发生中所处的地位.

  4. 人乳头瘤病毒衣壳蛋白与宫颈病变%Human Papillomavirus′ Capsid Proteins and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    黄成琳; 张淑兰

    2014-01-01

    Cervical cancer seriously endangers women′s health,and human papillomavirus (HPV) is considered to be the primary cause. Doctors have been striving to find an effective diagnostic method for judging cervical lesions level and predicting its prognosis. HPV capsid proteins comprise the major capsid protein (L1 capsid protein) and the minor capsid protein (L2 capsid protein),and these two proteins play an important role in assembling into virus particles,trafficking HPV to the cell,and causing the host′s immune reactions. In recent years,studies have shown that the L1 capsid protein can be used to predict the progress and subsidence of cervical lesions. HPV prophylactic vaccines ,which are exploited on the basis of the L1 and L2 capsid protein,are proved to get a good preventive effect in clinical trials. This paper reviews the biological characteristics of HPV and researches progress on HPV capsid protein in cervical lesions in recent years.%宫颈癌严重危害妇女健康,人乳头瘤病毒(HPV)感染是其首要病因。临床医师一直致力于寻找一种能有效判断宫颈病变级别及预测预后的诊断方法。 HPV衣壳蛋白包括主要衣壳蛋白(L1壳蛋白)和次要衣壳蛋白(L2壳蛋白),这两种蛋白在组装成病毒颗粒、协助病毒入胞及引起机体免疫反应等多个方面发挥重要作用。近年研究表明, L1壳蛋白可用于预测宫颈病变的进展与消退。以L1及L2壳蛋白为基础研发的HPV预防性疫苗在临床试验中得到了很好的预防效果。综述HPV生物学特点及近年来有关HPV衣壳蛋白在宫颈病变的研究进展。

  5. Detection of Human Papilloma Virus (HPV in oral mucosa of women with cervical lesions and their relation to oral sex practices

    Directory of Open Access Journals (Sweden)

    Martinez-Martinez Alejandro

    2010-12-01

    Full Text Available Abstract Background Previous studies have either investigated the relationship of HPV with oral cancer or the prevalence of HPV on the oral cavity. The purpose of this investigation was to study the prevalence of HPV in oral cavity of women with oral sex practices and cervical lesions. Methods Forty six (46 non-smokers and non-alcoholic patients attended the "Clínica de Displasias" of "Ciudad Juarez" were sampled. This population had a CIN diagnosis sometime between the previous six months. On previous consent they filled out a questionnaire related to their oral sex practices. Afterwards one swab from cheeks and another from palate/gum were taken; PCR was used to determine generic HPV, HPV16 and HPV18. Results Seventy two percent (72% of the patients stated to have oral sex practices regularly which all of them were positive to HPV either in oral mucus, palate/gum or both. The total of the given results showed that 35% had HPV16; among those distributed in 26% with regular oral sex practices and 9% stated as never practiced oral sex. An association was found between oral HPV16 positivity and progression to cervical CIN advanced lesions. On the other hand HPV18 was not detected. The frequency of HPV16 was higher in buccal mucosa (23% versus palate/gum (16%. Conclusions This study suggests that buccal HPV16 infection is associated with CIN progression.

  6. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion

    Science.gov (United States)

    de Abreu, André LP; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria DB; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia EL

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  7. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.

    Science.gov (United States)

    de Abreu, André Lp; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria Db; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia El

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention.

  8. Diagnosing Cervical Dysplasia Using Visual Inspection of the Cervix with Acetic Acid in a Woman in Rural Haiti

    Directory of Open Access Journals (Sweden)

    Elizabeth Roger

    2014-11-01

    Full Text Available Cervical cancer remains a significant cause of morbidity and mortality for women in developing countries, despite the fact that inexpensive, simple and effective screening methods are available. Visual inspection of the cervix with acetic acid (VIA can be used as part of a “screen and treat” program to identify precancerous lesions for cryotherapy treatment. This case report details how the VIA screening test was incorporated into the care of a patient presenting to a maternal health clinic in Thomonde, Haiti which was staffed by doctors and medical students from Emory University School of Medicine in collaboration with Haiti Medishare. As demonstrated here, the VIA test requires minimal materials, can be efficiently incorporated into a physical exams, provides immediate results, and is easily demonstrated to and performed by local healthcare providers. The straightforward and sensitive VIA technique is an ideal cervical cancer screening method for resource poor areas.

  9. Risk of progression of early cervical lesions is associated with integration and persistence of HPV-16 and expression of E6, Ki-67, and telomerase

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    Arianna Vega-Peña

    2013-01-01

    Full Text Available Background: Low-grade squamous intraepithelial lesions (LSIL are the earliest lesions of the uterine cervix, the persistence and integration of high-risk human papillomavirus (HR-HPV as type 16, which promotes the development of more aggressive lesions. Aim: To select more aggressive lesions with tendency to progress to invasive cervical cancer. Materials and Methods: A total of 75 cytological specimens in liquid base (Liqui-PREP were analyzed: 25 specimens were with no signs of SIL (NSIL and without HPV; 25 NSIL with HPV-16, and 25 with both LSIL and HPV-16. The expression of Ki-67, telomerase, and viral E6 was evaluated by immunocytochemistry; and the detection of viral DNA was done by polymerase chain reaction (PCR and restriction fragment length polymorphism (RFLPs for genotyping or sequencing of HPV-16. The physical state of HPV-16 was evaluated by in situ hybridization with amplification with tyramide. Results: Of the total group, 58.6% had LSIL associated with persistence and of these 59.3% was associated with integrated state of HPV as intense expression of E6, Ki-67 (P = 0.013, P = 0.055 has except for the expression of telomerase present a non-significant association (P<0.341. Conclusions: Overexpression of E6 and Ki-67 is associated with the integration of HPV-16, favoring viral persistence, and increasing the risk of progression in women with NSIL and LSIL.

  10. Clinical value of TCT combined with HPV-DNA detection in cervical cancer screening%液基薄层细胞学联合 HPV-DNA检测对宫颈癌患者筛查的临床价值

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2016-01-01

    Objective:To explore clinical value ThinPrep cytology test ( TCT) and HPV detection in cervical cancer screen-ing. Methods:The clinical data of the patients in the screening of cervical precancerous lesions were collected. Each patient was given TCT combined with HPV detection. Finally, after colposcopy cervical biopsy, the sensitivity, accuracy and false positive rate of single TCT, single HPV and TCT combined with HPV in the diagnosis of chronic cervical inflammation, cervical precancerous lesions and cervical cancer were compared. Results:The cervical cancer and precancerous lesions screening under colposcopy biopsy was done in 360 patients. Among the 3 screening methods, the combined application of TCT and HPV had the highest sensitivity and specificity, and could be used for diagnosing severe atypical hyperplasia ( CIN-III) and cervical carcinoma without missed diagnosis;however, it might miss some moderate dysplasia ( CIN-II) and mild atypical hyperplasia ( CIN-I) patients. Conclusions:The TCT combined with HPV-DNA detection can be used actively in the cervical precancerous lesions screening, and improve the accuracy of clinical diagno-sis. Moreover, combined with colposcopy biopsy of cervical cancer and precancerous lesions, it can provide a diagnostic basis for the clinical treatment, and is worthy of clinical application.%目的::探讨液基薄层细胞学与HPV检测联合应用后在宫颈癌患者筛查中的临床应用价值。方法:分析行宫颈癌前病变筛查的患者共360例临床资料,每位患者给予薄层液基细胞学联合HPV检测,最后经阴道镜下宫颈活检,比较单独TCT检查、单独HPV检查及两种检查联合检测在诊断患者宫颈慢性炎症、宫颈癌前病变及宫颈癌的敏感程度、准确性和假阳性率。结果:宫颈癌及癌前病变筛查患者,行阴道镜下活检的患者360例。3种筛选方法中,联合应用TCT及HPV的敏感性、特异性最

  11. ANTIGEN MG7 IN GASTRIC CANCER AND GASTRIC PRECANCEROUS LESIONS

    Institute of Scientific and Technical Information of China (English)

    郭冬丽; 宁佩芳; 袁媛

    2004-01-01

    Objective: To study the dynamic change and its diagnostic significance of MG7 expression in the process of gastric cancer development. Methods: The expression level of antigen MG7 was determined by immunohistochemistry method in 406 cases of gastric mucosa. The classification of intestinal metaplasia of gastric mucosa was determined by histochemistry method in 82 cases. Results: The positive rate of MG7 expression in normal gastric mucosa, intestinal metaplasia and dysplasia of gastric mucosa and gastric cancer were increased gradually (P<0.01). The positive rate of MG7 expression in superficial gastritis, atrophic gastritis and gastric cancer were increased on sequence (P<0.01). The positive rate of antigen MG7 expression in type Ⅲ intestinal metaplasia of gastric mucosa had significant difference,compared with that in type Ⅰ an Ⅱ intestinal metaplasia (P<0.05). Conclusion: MG7 antigen had close relationship with gastric cancer. Type Ⅲ intestinal metaplasia, atrophic gastritis and dysplasia should be followed up in order to improve the early detection of gastric cancer. MG7 antigen had great clinical value in the dynamic follow-up of gastric precursors.

  12. Electrocautery Superior to Topical Treatments for Precancerous Anal Lesions

    Science.gov (United States)

    ... Ulrike Wieland, MD, of the University of Cologne, Germany, and Alexander Kreuter, MD, of HELIOS St. Elisabeth Klinik in Oberhausen, Germany, in an accompanying editorial . Based on the trial’s ...

  13. Pre-cancerous (DNA and chromosomal lesions in professional sports

    Directory of Open Access Journals (Sweden)

    Radhika Sharma

    2012-01-01

    Conclusion: Significantly increased genomic instability in players of both sports was observed. Both repaired and repairable genetic damage cells were observed in different tissues of the same subject. The presence of such genetic damage implies that these players are at an individual risk from cancer- and age-related diseases.

  14. Mitochondrial microsatellite instability in gastric cancer and its precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Xian-Long Ling; Dian-Chun Fang; Rong-Quan Wang; Shi-Ming Yang; Li Fang

    2004-01-01

    AIM: To evaluate the role of mitochondrial microsatelliteinstability (mtMSI) in gastric carcinogenesis. METHODS: MtMSI was measured with PCR-single strand conformation polymophism (PCR-SSCP) in 68 cases of advanced gastric cancer, 40 cases of chronic gastritis, 30 cases of intestinal metaplasia and 20 cases of dysplasia.RESULTS: MtMSI was observed in 12.5% (5 of 40) of chronic gastritis, 20.0% (6 of 30) of intestinal metaplasia, 25.0% (5 of 20) of dysplasia and 38.2% (26 of 68) of gastric cancer. These findings showed a sequential accumulation of mtMSI in the histological progression from chonic gastritis to gastric cancer. An association of mtMSI with intestinal histological type and distal location was found (P=0.001 and P=0.002), whereas no significant correlation was found between mtMSI and age at diagnosis, sex, tumor size, depthof invasion, lymph node spread and clinical stages (P>0.05).CONCLUSION: MtMSI may play an early and importantrole in the gastric carcinogenesis pathway, especially in the intestinal type and distal gastric cancer.

  15. HPV infection subtype distribution and its relation with cervical lesions%HPV 感染亚型分布及其与宫颈病变关系的研究

    Institute of Scientific and Technical Information of China (English)

    段山红; 安瑞芳

    2015-01-01

    among gynecological outpatients in Xianyang district and its relation with cervical lesions.Methods Between January 2009 and February 2013, totally 3 573 outpatients undergoing HPV genotyping and liquid-based cytology check were selected in three tertiary hospitals in Xianyang district.There were 966 cases with HPV genotype positive ( including 19 kinds of high-and low-risk subtype) and 572 cases with cytological positive ( ASCUS) .Both positive results were found in 354 cases.For 1 169 patients with any positive results, colposcopy and cervical biopsy were performed.The correlation between subtypes of HPV infection and cervical lesions was analyzed according to pathological examination results.Results HPV testing positive rate was 27.04%.HR-HPV positive rate was 24.55%, and HPV16 positive rate was 12.26% accounting for 45.34% of HR-HPV.The highest positive rate of HPV was found among women aged below 30.With age increasing, HPV positive rate declined gradually.There were significant differences in HPV positive rate among different age groups except for group aged≤20 years and group aged 21-30 years (χ2 value was 5.08, 8.93, 37.48 and 4.28, respectively, all P<0.05).The detection rates of HPV16 in CINⅡ, CINⅢand squamous cell cancer (SCC) were highest, and they were 50.59%, 78.13%and 81.82%, respectively.Compared with HPV58, HPV18, HPV52 and HPV33, HPV16 caused CINⅡor above more easily, and the difference was statistically significant (χ2 value was 53.93,62.84,93.01 and 93.01, respectively, all P<0.05).Conclusion The most common high-risk HPV genotypes infected in outpatients in Xianyang district are HPV16, HPV52, HPV58, HPV18 and HPV33.HPV16 plays a key role in the genesis and development of cervical cancer and precancerous lesions.HPV58, HPV18, HPV52, and HPV33 are also pathogenic in this area.High-risk HPV genotyping is therefore very important in cervical cancer screening and the process of prevention and diagnosis.

  16. The influence of human papillomavirus type and HIV status on the lymphomononuclear cell profile in patients with cervical intraepithelial lesions of different severity

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    Donadi Eduardo A

    2009-08-01

    Full Text Available Abstract Background Immunological alterations are implicated in the increased prevalence of high-grade squamous intraepithelial lesions (HG-SIL and persistent human papillomavirus (HPV infection. This study evaluated the expression of CD4, CD8, CD25 (IL-2Rα and CD28 antigens from SIL biopsies, stratified by HIV status and HPV-type. Biopsies specimens from 82 (35 HIV+ women with a normal cervix, low-grade (LG-SIL or high-grade lesions (HG-SIL were studied. CD molecule expression was evaluated by immunohistochemistry and HPV detection/typing performed using PCR techniques. Results CD4 stromal staining was increased in patients with HPV18. Women with HPV16 infection showed decreased: a CD8 and CD25 stromal staining, b CD25 staining in LG-SIL epithelium and in HG-SIL stroma. In HIV- women samples, CD28 epithelial staining and CD8 stromal staining surrounding metaplastic epithelium were less intense and even absent, as compared to HIV+ women. Both epithelial and stromal CD8 staining was more intense in the HG-SIL/HIV+ group than in the HG-SIL/HIV- group. Positive correlations were observed between CD4/CD25, CD4/CD28 and CD25/CD28 in the stroma and CD25/CD28 in the epithelium. Conclusion HIV status and HPV-type may influence the lymphomononuclear cell profile present in the spectrum of cervical lesions. The knowledge of the infiltrating cell profile in cervical tumours may help the development of specific anti-tumoural strategies.

  17. Prevalence of Human Papillomavirus Types Among Mexican Women with Intraepithelial Lesions and Cervical Cancer: Detection with MY09/MY011 and GP5+/GP6+ Primer Systems

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    Gloria Fernández-Tilapa

    2007-01-01

    Full Text Available Squamous cervical carcinoma (SCC is the most common cancer found in Mexican women. Human papillomavirus (HPV infection is a prerequisite for this disease. In Mexico little is known about the prevalence of HPV-types and knowledge of the circulating genotypes by region is limited. The aim of this study was to determine the prevalence and genotypes of HPV in biopsies from women with intraepithelial lesions and SCC. A total of 211 cervical biopsies were studied. Histopathological analysis was done and HPV DNA was detected by PCR using MY09/MY11 (MY-PCR and GP5+/GP6+ (GP+PCR primers. Viral type was determined by RFLP’s or sequencing. Tissues were classified as low-grade squamous intraepithelial lesion (LSIL in 123 cases, high-grade squamous intraepithelial lesion (HSL in 40 and SCC in 48. Prevalence of HPV was 55.3% in LSIL, 65% in HSIL and 91.7% in SCC by MY-PCR. These percentages increased to 83, 77.5 and 100%, respectively, when HPV-negative samples were analyzed by GP+PCR. In 138 of 211 samples (65.4%, HPV was detected by MY-PCR and 43 (20.4% were positive by GP+PCR. In 166 (91.7% of 181 infections high risk HPV-types were found. Twelve genotypes were detected (16, 18, 31, 33, 45, 52, 58, 59, 61, 66, 70, 81. Prevalence of HPV 16 in LSIL, HSIL and SCC, were 70.6, 54.8 and 70.8%, respectively and it was the most common type in all cases (67.9%. A significantly higher number of positive samples were detected with MY-PCR and GP+PCR. The high prevalence of HPV infection with high-risk types, especially HPV16, among Mexican women with SIL and SCC, has important implications in the treatment and prophylaxis.

  18. Targeting immune response with therapeutic vaccines in premalignant lesions and cervical cancer: hope or reality from clinical studies.

    Science.gov (United States)

    Vici, P; Pizzuti, L; Mariani, L; Zampa, G; Santini, D; Di Lauro, L; Gamucci, T; Natoli, C; Marchetti, P; Barba, M; Maugeri-Saccà, M; Sergi, D; Tomao, F; Vizza, E; Di Filippo, S; Paolini, F; Curzio, G; Corrado, G; Michelotti, A; Sanguineti, G; Giordano, A; De Maria, R; Venuti, A

    2016-10-01

    Human papillomavirus (HPV) is widely known as a cause of cervical cancer (CC) and cervical intraepithelial neoplasia (CIN). HPVs related to cancer express two main oncogenes, i.e. E6 and E7, considered as tumorigenic genes; their integration into the host genome results in the abnormal regulation of cell cycle control. Due to their peculiarities, these oncogenes represent an excellent target for cancer immunotherapy. In this work the authors highlight the potential use of therapeutic vaccines as safe and effective pharmacological tools in cervical disease, focusing on vaccines that have reached the clinical trial phase. Many therapeutic HPV vaccines have been tested in clinical trials with promising results. Adoptive T-cell therapy showed clinical activity in a phase II trial involving advanced CC patients. A phase II randomized trial showed clinical activity of a nucleic acid-based vaccine in HPV16 or HPV18 positive CIN. Several trials involving peptide-protein-based vaccines and live-vector based vaccines demonstrated that these approaches are effective in CIN as well as in advanced CC patients. HPV therapeutic vaccines must be regarded as a therapeutic option in cervical disease. The synergic combination of HPV therapeutic vaccines with radiotherapy, chemotherapy, immunomodulators or immune checkpoint inhibitors opens a new and interesting scenario in this disease.

  19. The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Li J

    2016-08-01

    Full Text Available Jinyun Li,1,2,* Chongchang Zhou,1,* Haojie Zhou,3,* Tianlian Bao,1 Tengjiao Gao,1 Xiangling Jiang,1 Meng Ye1,2 1Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, 2Department of Medical Oncology, Affiliated Hospital, Ningbo University, 3Department of Molecular Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang, People’s Republic of China *These authors are co-first authors of this work Background: Cervical cancer is the second deadliest gynecologic malignancy, characterized by apparently precancerous lesions and cervical intraepithelial neoplasia (CIN, and having a long course from the development of CIN to cervical cancer. Cyclin-dependent kinase inhibitor 2A (CDKN2A is a well-documented tumor suppressor gene and is commonly methylated in cervical cancer. However, the relationship between methylated CDKN2A and carcinogenesis in cervical cancer is inconsistent, and the diagnostic accuracy of methylated CDKN2A is underinvestigated. In this study, we attempted to quantify the association between CDKN2A methylation and the carcinogenesis of cervical cancer, and its diagnostic power.Methods: We systematically reviewed four electronic databases and identified 26 studies involving 1,490 cervical cancers, 1,291 CINs, and 964 controls. A pooled odds ratio (OR with corresponding 95% confidence intervals (95% CI was calculated to evaluate the association between methylated CDKN2A and the carcinogenesis of cervical cancer. Specificity, sensitivity, the area under the receiver operating characteristic curve, and the diagnostic odds ratio were computed to assess the effect of methylated CDKN2A in the diagnosis of cervical cancer.Results: Our results indicated an upward trend in the methylation frequency of CDKN2A in the carcinogenesis of cervical cancer (cancer vs control: OR =23.67, 95% CI =15.54–36.06; cancer vs CIN: OR =2.53, 95% CI =1.79–3.5; CIN vs control: OR =9.68, 95% CI =5.82–16.02. The

  20. Correlation of DNA Ploidy with Progression of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    M. Singh

    2008-01-01

    Full Text Available The majority of squamous cell carcinomas of cervix are preceded by visible changes in the cervix, most often detected by cervical smear. As cervical cancer is preceded by long precancerous stages, identification of the high-risk population through detection of DNA ploidy may be of importance in effective management of this disease. Here we attempted to correlate aneuploid DNA patterns and their influence on biological behavior of flow-cytometry analysis of DNA ploidy which was carried out in cytologically diagnosed cases of mild (79, moderate (36, and severe (12 dysplasia, as well as “atypical squamous cells of unknown significance (ASCUS” (57 along with controls (69, in order to understand its importance in malignant progression of disease. Cytologically diagnosed dysplasias, which were employed for DNA ploidy studies, 39 mild, 28 moderate, and 11 severe dysplasia cases were found to be aneuploid. Out of the 69 control subjects, 6 cases showed aneuploidy pattern and the rest 63 subjects were diploid. An aneuploidy pattern was observed in 8 out of 57 cases of cytologically evaluated ASCUS. The results of the followup studies showed that aberrant DNA content reliably predicts the occurrence of squamous cell carcinoma in cervical smear. Flow cytometric analysis of DNA ploidy may provide a strategic diagnostic tool for early detection of carcinoma cervix. Therefore, it is a concept of an HPV screening with reflex cytology in combination with DNA flow cytometry to detect progressive lesions with the greatest possible sensitivity and specificity.

  1. Risk of cervical cancer and precancerous lesions in patients infected with human papilloma virus%人乳头状瘤病毒感染致宫颈癌及癌前病变风险研究

    Institute of Scientific and Technical Information of China (English)

    严敏婵; 张晓雪; 陈东; 陈星; 袁兆林; 卢洪胜; 沈波

    2010-01-01

    目的 探讨感染人乳头状瘤病毒(human papilloma virus,HPV)后患宫颈癌及癌前病变风险.方法 采用HPV-DNA核酸扩增及导流杂交技术,对7 234例来我院就诊的宫颈异常患者,进行HPV21种亚型的基因检测和宫颈液基薄层细胞学(TCT)检测,对细胞学检测结果为非典型鳞状细胞(ASCUS)以上和/或HPV-DNA检测阳性的患者,行活组织病理检查,对HPV感染后患宫颈癌及癌前病变风险进行分析.结果 HPV总感染率为27.37%,检出率前四位依次是HPV 16型6.97%(504/7 234)、52型5.68%(411/7 234)、58型5.27%(381/7 234)和33型3.33%(241/7 234),其中高危型病毒(HR-HPV)感染占78.16%(2147/2747).宫颈癌患者HPV的检出率为73.33%(33/45).单型HPV感染后患轻度鳞状上皮内瘤变(LSIL)及高度鳞状上皮内瘤变(HSIL)和宫颈癌的风险分别是HPV阴性者的4.055倍(95% CI 2.528~6.505)和5.103倍(95% CI 3.838~6.786),多型混合HPV感染后患宫颈LSIL及HSIL和宫颈癌的风险分别是HPV阴性者的7.313倍(95% CI 4.314~12.397)和7.550倍(95%CI 5.395~10.564).结论 单型HPV感染致宫颈HSIL或宫颈癌的风险是HPV阴性者的5.1倍,多型混合HPV感染致宫颈HSIL或宫颈癌的风险是HPV阴性者的7.6倍.

  2. Expression of Pepsinogen C in Gastric Cancer and Precancerous Diseases and its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    PeifangNing; UpingSun; HuijieLiu; YuanYuan

    2004-01-01

    OBJECTIVE To investigate the active expression of pepsinogen C (PGC) and its value in detection of precancerous diseases and gastric cancer.METHODS Immunohistochemistry was used to examine the expression of pepsinogen C in 424 specimens of gastric mucosa collected by gastroscopy.RIFSULTS The positive rate of PGC expression in 54 cases of normal gastric mucosa was 100 % and 2.4% in 124 cases of gastric cancer. The positive rate of PGC expression in superficial gastritis, gastric ulcer or erosion, atrophic gastritis or gastric dysplasia and gastric cancer decreased significantly in the sequence indicated (P<0.05).CONCLUSION The expression of PGC is negatively correlated with the degree of malignancy of gastric mucosa and with development of gastric lesions. PGC expression has a high sensitivity and specificity for diagnosis of precancerous diseases which can lead to gastric cancer and may be a good indicator for screening and diagnosis of gastric cancer and precursors of gastric cancer.

  3. Risk factors of neurological lesions in low cervical spine fractures and dislocations Fatores de risco de lesão neurológica nas fraturas e luxações da coluna cervical baixa

    Directory of Open Access Journals (Sweden)

    DANILO GONÇALVES COELHO

    2000-12-01

    Full Text Available Eighty-nine patients with lower cervical spine fractures or dislocations were evaluated for risk factors of neurological lesion. The age, sex, level and pattern of fracture and sagittal diameter of the spinal canal were analysed. There were no significant differences on the age, gender, level and Torg's ratio between intact patients and those with nerve root injury, incomplete or complete spinal cord injuries. Bilateral facet dislocations and burst fractures are a significant risk factor of spinal cord injury.O risco de lesão neurológica foi avaliado em 89 pacientes com fraturas ou luxações da coluna cervical baixa. Foram analisados o sexo, a idade, o diâmetro sagital do canal vertebral, o nível e o tipo de fratura ou luxação. Não foram encontradas diferenças significativas para a idade, sexo, nível da fratura ou luxação e razão de Torg entre os pacientes intactos e aqueles com lesão radicular, lesão medular incompleta e lesão medular completa. Luxações bilaterais de facetas articulares e fraturas em explosão são um fator de risco significativo de lesão neurológica.

  4. Multifocal epithelial hyperplasia: A potentially precancerous disease? (Review)

    Science.gov (United States)

    BASCONES-MARTÍNEZ, A.; COK, S.; BASCONES-ILUNDÁIN, C.; ARIAS-HERRERA, S.; GOMEZ-FONT, R.; BASCONES-ILUNDÁIN, J.

    2012-01-01

    Multifocal epithelial hyperplasia (MEH), also known as Heck’s disease, manifests as a papulonodular lesion in the oral mucosa and has been associated with the human papillomavirus, a virus related to various precancerous diseases in the oral cavity. It has a predisposition for the female gender and for children. Although the majority of reported cases have been among American Indians and Eskimos, it has been described in multiple ethnic groups in various geographical locations. The objective of this review was to report on the clinical characteristics and epidemiology of MEH and its possible correlation with oral cancer. It is based on a search of articles in international journals published prior to April 2011, using the PubMed database and selecting articles related to the epidemiology and clinical characteristics of MEH. The review revealed a higher number of cases in individuals of American Indian origin and a predilection of the disease for the female gender and for patients between the 1st and 2nd decades of life. The most frequent lesion site was the lower lip. The disease has been associated with socio-economic and genetic factors, among others. No cases of malignant transformation have been reported. PMID:22740890

  5. Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil

    Directory of Open Access Journals (Sweden)

    Geni NL Camara

    2003-10-01

    Full Text Available As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061 were less frequent (= or < 2.5% each. The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.

  6. Promoter hypermethylation-mediated inactivation of multiple Slit-Robo pathway genes in cervical cancer progression

    Directory of Open Access Journals (Sweden)

    Mansukhani Mahesh

    2006-05-01

    Full Text Available Abstract Background Cervical Cancer (CC exhibits highly complex genomic alterations. These include hemizygous deletions at 4p15.3, 10q24, 5q35, 3p12.3, and 11q24, the chromosomal sites of Slit-Robo pathway genes. However, no candidate tumor suppressor genes at these regions have been identified so far. Slit family of secreted proteins modulates chemokine-induced cell migration of distinct somatic cell types. Slit genes mediate their effect by binding to its receptor Roundabout (Robo. These genes have shown to be inactivated by promoter hypermethylation in a number of human cancers. Results To test whether Slit-Robo pathway genes are targets of inactivation at these sites of deletion, we examined promoter hypermethylation of SLIT1, SLIT2, SLIT3, ROBO1, and ROBO3 genes in invasive CC and its precursor lesions. We identified a high frequency of promoter hypermethylation in all the Slit-Robo genes resulting in down regulated gene expression in invasive CC, but the inhibitors of DNA methylation and histone deacetylases (HDACs in CC cell lines failed to effectively reactivate the down-regulated expression. These results suggest a complex mechanism of inactivation in the Slit-Robo pathway in CC. By analysis of cervical precancerous lesions, we further show that promoter hypermethylation of Slit-Robo pathway occurs early in tumor progression. Conclusion Taken together, these findings suggest that epigenetic alterations of Slit-Robo pathway genes (i play a role in CC development, (ii further delineation of molecular basis of promoter methylation-mediated gene regulation provides a potential basis for epigenetic-based therapy in advanced stage CC, and (iii form epigenetic signatures to identify precancerous lesions at risk to progression.

  7. 人乳头状瘤病毒基因亚型与宫颈病变的关系%The Relationship between Human Papillomavirus Subgenotype and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    张淑群

    2014-01-01

    Objective To study the relationship between human papilloma virus ( HPV-DNA) subgenotype infection and severity of cervical lesions .Methods 140 cases of HPV infection were selected as the study object .Cervical secretions were taken for viral genotyping ,21 kinds of HPV-DNA subtype analysis was performed by rapid hybridization using nucleic acid mole-cule genotyping technology ,all subjects received lower cervical TCT detection (LCT)and colposcopical multiple biopsy pathology . Under pathological diagnosis ,patients were divided into with normal or inflammation group ,low-grade squamous intraepithelial le-sion (LSIL)group,high-grade squamous intraepithelial lesion (HSIL)group.The relationship between HPV infection and cervical lesions was analyzed according to distribution of HPV-DNA subgenotype .Results 16 kinds of high-risk HPV mRNA isoforms were detected.The common genetic subtypes were HPV-16,58,52,18,33,31,CP8304,and HPV-16 had the highest detection rate.HPV-16 detection rates of the normal or inflammation group ,LSIL group and HSIL group were 19.23%,23.53%,65.22%, detection rate of HPV-16 in HSIL group was significantly higher than those of the other 2 groups .5 kinds of low-risk HPV genetic subtypes were detected,and HPV-11 was the most common.Detection rate of multiple HPV infection in HSIL group was 54.35%, which was significantly higher than that of the other 2 groups,P<0.05,there had statistical significance .Conclusion HPV-16, 58,52,18,33,31,CP8304 infection are the most common in cervical lesions .HPV genotyping assay is of great value for the diag-nosis and prognosis of cervical lesions ,and it is worthy of clinical application .%目的:研究人乳头状瘤病毒基因亚型( HPV-DNA)感染情况及其与宫颈病变程度的关系。方法选取140例HPV感染患者作为临床研究对象。取患者宫颈分泌物作病毒分型,采用核酸分子快速杂交基因分型技术进行21种HPV-DNA亚型分析,所有研究对象均行宫

  8. A placebo-controlled randomized HPV16 synthetic long-peptide vaccination study in women with high-grade cervical squamous intraepithelial lesions.

    Science.gov (United States)

    de Vos van Steenwijk, Peggy J; Ramwadhdoebe, Tamara H; Löwik, Margriet J G; van der Minne, Caroline E; Berends-van der Meer, Dorien M A; Fathers, Lorraine M; Valentijn, A Rob P M; Oostendorp, Jaap; Fleuren, Gert Jan; Hellebrekers, Bart W J; Welters, Marij J P; van Poelgeest, Mariette I; Melief, Cornelis J M; Kenter, Gemma G; van der Burg, Sjoerd H

    2012-09-01

    The aim of this study was to investigate the capacity of an HPV16 E6/E7 synthetic overlapping long-peptide vaccine to stimulate the HPV16-specific T-cell response, to enhance the infiltration of HPV16-specific type 1 T cells into the lesions of patients with HPV16+ high-grade cervical squamous intraepithelial lesion (HSIL) and HPV clearance. This was a placebo-controlled randomized phase II study in patients with HPV16-positive HSIL. HPV16-specific T-cell responses were determined pre- and post-vaccination by ELISPOT, proliferation assay and cytokine assays in PBMC and HSIL-infiltrating lymphocytes, and delayed-type hypersensitivity skin tests. Motivational problems of this patient group to postpone treatment of their premalignant lesions affected the inclusion rates and caused the study to stop prematurely. Of the accrued patients, 4 received a placebo and 5 received 1-2 vaccinations. Side effects mainly were flu-like symptoms and injection site reactions. A strong HPV-specific IFNγ-associated T-cell response was detected by ELISPOT in all vaccinated patients. The outcome of the skin tests correlated well with the ELISPOT analysis. The cytokine profile associated with HPV16-specific proliferation varied from robust type 1 to dominant type 2 responses. No conclusions could be drawn on vaccine-enhanced T-cell infiltration of the lesion, and there was no HPV clearance at the time of LEEP excision. Thus, vaccination of HSIL patients results in increased HPV16-specific T-cell immunity. Further development of this type of treatment relies on the ability to motivate patients and in the reduction in the side effects.

  9. Comparison of conventional Papanicolaou smear and SurePath® liquid-based cytology in the Copenhagen population screening programme for cervical cancer

    DEFF Research Database (Denmark)

    Kirschner, Benny; Simonsen, Kåre; Junge, Jette

    2006-01-01

    OBJECTIVE: To compare diagnostic performance of conventional Papanicolaou smear with SurePath liquid-based cytology in a population screening programme. METHODS: A retrospective comparison was performed on data from two 18-month periods of the screening programme for cervical cancer...... in the municipality of Copenhagen with conventional Papanicolaou technique (n = 82,116) and liquid-based cytology (n = 84,414). RESULTS: After the conversion to liquid-based cytology the percentage of unsatisfactory samples decreased from 2.3% to 0.3% (P ...-based technique. CONCLUSIONS: This study showed the number of unsatisfactory samples to be significantly reduced with the liquid-based technique. The data suggest that there is an increased detection rate of cervical precancerous lesions with liquid-based cytology, but the number of false positive tests is still...

  10. Expression and Significance of Treg Cells and IDO in a Spectrum of HPV-Induced Cervical Lesions%Treg细胞和IDO在HPV感染相关宫颈疾病组织中表达及意义

    Institute of Scientific and Technical Information of China (English)

    蒋艳萍; 曹来英; 洛若愚

    2012-01-01

    Objective To study the clinical significance of the level of regulatory T ( Treg ) cells and indoleamine 2,3-dioxygenase ( IDO ) in a Spectrum of HPV-Induced Cervical Lesions. Methods Human papillomavirus ( HPV ) testing, thinprep cytologic test ( TCT ) and cervical biopsy were performed in cervical mucosal from 194 women and then all patients were classified as normal cervices ( control subjects ), HPV-negative cervicitis, HPV-positive cervicitis, CIN I , CIN II , CINIH or cervical cancer. Flow cytometry was used to assess the percentages of Treg cells in CD4 + T cells in the cervical mucosal and quantitative PCR was used to analysis the cervical mRNA expression of IDO. Results Cervical cancer had higher numbers of Treg cells and expressed more IDO than other groups. The percentages of Treg cells in CD4 + T cells in the cervical mucosal were higher in CIN IH , CIN II , CIN I and HPV-positive cervicitis than what in HPV- negative cervicitis and control subjects. The expressions of IDO were significantly higher in CINIH and CIN II than what in CIN I , HPV-positive cervicitis, HPV-negative cervicitis and control subjects. Conclusion The development of HPV-induced cervical Lesions is assoiated with HPV persistenced infection and mucosal enrichment of Treg cells. IDO is over expressed in HPV-Induced CINIH ,CIN II and Cervical cancer. Treg cells and IDO may associated with cervical cancer immune escape mechanisms.%目的 探讨HPV感染相关宫颈疾病患者宫颈组织中Treg细胞和吲哚胺2,3-二氧酶(IDO)的表达及其临床意义.方法 对194例患者行HPV检测、宫颈TCT检查及宫颈病理学检查,然后按病变程度分为正常宫颈(对照组)、HPV阴性(HPV-)宫颈炎、HPV阳性(HPV+)宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌6组实验组,应用Q-PCR、流式细胞术,检测各组宫颈组织中Treg细胞和吲哚胺2,3-二氧酶(IDO)的表达水平.结果 宫颈癌中Treg细胞占CD4+ T细胞的比例及IDO表达

  11. Expression of CXC chemokine 10 in chronic non-atrophy gastritis,carcinoma of stomach precancerous lesions and gastric cancer and their clinical significance%趋化因子10在慢性非萎缩性胃炎、胃癌癌前病变及胃癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    何甜; 唐慧; 郭强; 杨慧

    2014-01-01

    Objective To investigate the expressions of CXC chemokine 10(CXCL10) in chronic non-atrophy gastritis(CNAG) , precancerous lesions(PL)and gastric cancer(GC) ,primitively understanding of CXCL10 expression levels in three gastric types ,ex-ploring their clinical significances .Methods The expressions of CXCL10 in 20 cases of CNAG ,60 cases of PL ,60 cases of GC tis-sues were examined with immunohistochemistry method ,the expression level of CXCL10 was analyzed by computer-assisted image analysis system ,and then analyzed statistically .Results CXCL10 expression were positive in parts of CNAG ,PL and GC the posi-tive rates were 10 .00% ,26 .67% ,71 .67% respectively) .Expression levels of CXCL10 in the GC tissue specimens were significant-ly higher than in CNAG and PL(P0 .05) .Expression levels of CXCL10 in CAG with IM ,CAG with Dys had no significant difference(P>0 .05) ,and in CAG with Severe Dys and Light-Moderate Dys had no significant difference(P>0 .05) .The expression levels of CXCL10 were rele-vant to the differentiation degree of GC (P>0 .05) .Conclusion The expression levels of CXCL10 were gradually rose from CNAG , PL to GC ,and had significant correlation with each other in CNAG ,PL and GC ,indicating that CXCL10 have a key role in the pro-duce and development of GC .%目的:检测趋化因子10(CXCL10)在慢性非萎缩性胃炎(CNAG)、癌前病变(PL)和胃癌(GC)中的表达情况,探讨CXCL10蛋白与GC临床特征及胃黏膜癌变机制的关系。方法应用免疫组织化学SP法检测CXCL10蛋白在20例CNAG、60例PL及60例GC患者胃黏膜中的表达。并应用计算机辅助图像分析仪对表达结果进行图像分析,然后进行统计学分析。结果CXCL10蛋白在CNAG、PL和GC中的表达阳性率分别为10.00%、26.67%、71.67%;CXCL10蛋白阳性表达率在GC组显著高于CNAG及PL组(均为 P<0.01);CXCL10蛋白在PL、CNAG两种病理组织中阳性表达率

  12. 胃癌及癌前病变中COX-2和NF-κBp65蛋白表达及其相关性研究%Study on the protein expression of COX-2 and of NF-κBp65 and its correlation in gastric cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    刘小玉; 冯义朝

    2013-01-01

    目的 观察幽门螺杆菌(Hp)、NF-κBp65、COX-2在胃黏膜癌变过程中的表达,探讨其表达与胃癌发生、发展的关系.方法 用免疫组化方法测定病例中COX-2、NF-κBp65蛋白的表达,用亚甲兰染色检测Hp.结果 ①慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、不典型增生(DYS)的Hp感染率与慢性浅表性胃炎(CSG)相比差异有显著性(x2=6.94,x2=6.71,x2=10.60,P<0.01).胃癌(GC)的Hp感染率与CSG相比差异无显著性(x2=1.37,P>0.05).②CAG、IM、DYS的COX-2表达率与CSG相比差异有显著性(x2 =4.81,x2=5.96,x2=16.57,P<0.05).GC的COX-2表达率与CSG相比差异亦有显著性(x2=34.50,P<0.01).③CAG、IM、DYS的NF-kBp65表达率与CSG相比差异有显著性(x2=4.71,x2=9.02,x2=20.74,P<0.05).GC的NF-kBp65表达率与CSG相比差异亦有显著性(x2=30.71,P<0.01).结论 Hp感染诱导COX-2和NF-kB表达发生在胃癌形成的早期阶段.COX-2和NF-kBp65参与了胃癌的发生发展过程,这些可能为胃癌的早期诊断及临床判断预后提供理论依据.%Objective To observe the expression of NF-kBp65 and COX-2 in gastric cancer and precancerous lesions, and to investigate the relationship between NF-kBp65 COX-2 expression and the pathogenesis of gastric cancer. Methods Slides were used for HE and sp immunohistochemical staining or Methylene Blue staining, the latter was used to detect the expression of NFkBp65, COX-2 and Hp infection. Results NF-kB and COX-2 expression is highly related with Hp infection. The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). Conclusion The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). NF-kBp65 and COX-2 expression is highly related with Hp infection. NF-kBp65 and COX-2 play important roles in the development progression of gastric carcinoma and NF-kBp65 may promote the expression of COX-2.

  13. 幽门螺杆菌感染及核转录因子-κBp65表达与胃癌的关系%Correlation between Hp infection and NF-κBp65 protein expression on gastric cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    刘小玉; 冯义朝

    2012-01-01

    Objective To investigate the relationship between helicobactor pylori infection and NF-?Bp65 expression in gastric cancer and precancerous lesions,and to study the possible mechanism of H. Pylori in gastric cancinogenesis. Methods 30 cases of chronic superficial gastritis specimens.30 cases of chronic atrophic gastritis specimens, 30 cases of intestina metaplasia specimens, 40 cases of dysplasia specimens and 80 cases of gastric carcinoma specimens were obtained Samples were fixed and embedded in paraffin as usual. Slides were used for HE and sp immunohistochemical staining or Methylene Blue staining,the latter was used to detect the expression of NF-?Bp65 and H. Pylori infection. NF-icBp65 expression is highly related with H. Pylori infection NF-icBp65expression is highly lrelated with H. Pylori infection. Results In CSG and GC,there was no significant difference in the positive rate of NF-icBp65 between H. Pylori positive patients and H. Pylori negative patients (P>0. 05). In CAG,IM and Dys .their expression level were intensive in H. Pylori positive patients when compared with H. Pylori negative patients(42. 1% vs 7. 7%,67. 9% vs 18. 2%,78. 6% vs 25. 0%). Conclusion The expression of NF-icBp65 is upregulated in the process of gastric cancer, NF-icBp65 possibly is helpful to the gastric cancer early diagnosis.%目的 探讨胃黏膜幽门螺杆菌(Helicobactor pylori,Hpylori)感染、核转录因子-κBp65(NF-κBp65)表达与胃癌发生、发展的关系.方法 胃癌(GC)标本80例,非典型增生(Dys)40例,萎缩性胃炎(CAG)30例,肠上皮化生(IM)30例,浅表性胃炎(CSG)30例,应用免疫组化方法测定NF-κBp65蛋白的表达,用亚甲兰染色检测Hpylori.结果 CSG、GC中Hpylori阳性和阴性病例NF-κBp65表达差异无显着性(P>0.05),但在CAG、IM、Dys的Hpylori阳性病例中NF-κBp65表达显著高于Hpylori阴性病例(42.1% vs 7.7%、67.9% vs 18.2%、78.6% vs 25.0%).结论 Hpylori感染诱导NF-κBp65表达发生在胃癌形成的早期阶段.

  14. 慢性萎缩性胃炎癌前病变患者报告临床结局评价量表的编制及信度、效度分析%Development and Reliability & Validity Analysis of Patient Reported Outcome Scale for Precancerous Lesion of Atrophic Gastritis

    Institute of Scientific and Technical Information of China (English)

    刘力; 常玉双; 沈舒文; 宇文亚;