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Sample records for cervical precancerous lesions

  1. Etiology and pathogenesis of precancerous lesions and invasive cervical carcinoma

    Directory of Open Access Journals (Sweden)

    Panjković Milana

    2008-01-01

    Full Text Available Cervical cancer is the second most common gynecological malignancy in the world. Human papilloma virus (HPV infection is the leading ethiologic agent in the development of premalignant and malignant cervical diseases. HPV is a member of the Papovaviridae family and until now over 100 types have been recognized. There are two types of viral infection: latent and productive. Virus induced oncogenesis is the result of interaction between virus oncoproteins E6 and E7 and tumor supresor host genes p53 and Rb. Many cofactors such as immunosuppression, early sexual relationship, multiple sexual partners, other sexualy transsmited infections and smooking are contributing factors of the precancerous and invasive cervical lesions. According to the oncogenic potential HPV are divided into three groups: low, intermediate and high oncogenic risk viruses. Molecular technics which are used for the virus detection are: In situ hibridisation,, Hybrid capture test and polymerasa chain reaction. Human papilloma virus testing has an important role in the follow up and treatment of women with 'atypical squamous cells of unknown significant' changes in cervical smears and low-grade squamous intraepithelial lesions, changes in punch biopsy.

  2. Prevalence of human papillomavirus types in cervical cancerous and precancerous lesions of Ecuadorian women.

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    Mejía, Lorena; Muñoz, Diana; Trueba, Gabriel; Tinoco, Leopoldo; Zapata, Sonia

    2016-01-01

    Human Papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it is responsible for most cases of uterine cancer. In Ecuador there is limited information about HPV types (and variants) in cancerous lesions; however, identifying the type-specific HPV prevalence in cervical lesions of women living in Ecuador is important to better predict the impact of HPV prophylactic vaccination in this country. We studied the prevalence of HPV types in cervical cancerous or precancerous lesions from 164 Ecuadorian women and found that 86.0% were HPV positive. The most common types were HPV16 (41.8%) and HPV58 (30.5%). Interestingly, HPV18 was detected only in 2.8% of the HPV-positive samples. Fifteen DNA sequences (genes E6 and L1) from 16 samples positive for HPV16 belonged to the European lineage, considered one of the least carcinogenic lineages, and 1 (6.25%) to the Asian-American lineage. Similar analysis in 12 HPV58 positive samples showed that 10 (83.3%) sequences grouped in sublineage A2, which belongs to the oldest HPV58 lineage, 1 belonged to A3 and 1 to lineage C. This study suggests that the currently used HPV vaccines (bivalent and tetravalent) may have lower effectiveness in Ecuador than in other geographic locations where HPV18 is more prevalent. PMID:26113443

  3. Relationship between hTERC gene and precancerous lesion as well as occurrence and development of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Li-Hong Yi

    2016-01-01

    Objective:To study the relationship betweenhTERC gene and precancerous lesion as well as occurrence and development of cervical cancer.Methods:A total of 120 cases receiving cervical biopsy in our hospital from May 2012 to December 2014 were selected for study, 45 cases of normal cervical tissue and inflammatory cervical tissue, 37 cases of cervical intraepithelial neoplasia tissue and 38 cases of cervical cancer tissue were included.hTERC gene expression, oncogene expression and invasive molecule contents in cervical tissue were detected.Results:Green signal copy number and red signal copy number ofhTERC in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue, and the contents of hTERTand hTP were not different from those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue with high expression of hTERC were significantly higher than those in cervical cancer tissue with low expression of hTERC.Conclusions:Activation of telomerase and increase of hTERC expression are involved in the precancerous lesion as well as the occurrence and development of cervical cancer, and hTERC can activate the expression of oncogenes and.

  4. Reliability of the CINtecTM p16INK4a immunocytochemical test in screening cervical precancerous lesions

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    Jović Milena

    2008-01-01

    Full Text Available Background/Aim. Overexpression of p16INK4a has been found to be linked with genomic integration of high-risk human papillomavirus (HPV and the developement of precancerous cervical intraepithelial lesions. The aim of this study was to examine is there a higher positive level of correlation between grade of histological dysplasia and p16INK4a level of expression in cervical smear, compared to results of Papanicolaou test. We also examined the correlation between HPV type, p16INK4a expression and Papanicolau test results. Methods. A total of 48 women with precanceorous cervical lesions and HPV cervicitis and 10 healthy women were enrolled in the study. Papanicolaou test, CINtecTM p16INK4a citological immunohistochemical test, polymerase chain reaction (PCR HPV 16, 18, 31, 33 analysis and histopathology of the lesion were performed in all the patients. Results. Comparing the results of Papanicoulaou test and the grade of histological dysplasia, low-grade squamous intraepithelial lesion (LSIL was confirmed in 38%, and high-grade squamous intraepithelial lesion (HSIL in 69.2% of the patients (p > 0.05. Significant positive correlation was found between p16 overexpression and grade of histological dysplasia (p = 0.000. Overexpression p16 was found in 70% of LSIL and 94.4% of HSIL. Positive correlation was found between p16 overexpression and grade of dysplasia in Papanicolaou test (p = 0.011. In 38% of LSIL and 15% of HSIL cases p16 was not expressed. The most frequently found HPV type in PCR analysis was HPV16. Analysing the results of p16 test according to HPV status and Papanicolaou test rather heterogenous results were obtained. Conclusion. In the patients with precancerous cervical lesions a higher level of correlation was found between the grade of histological dysplasia and p16INK4a level of expression in the cervical smear, compared to the results of Papanicolaou test.

  5. Prevalence and risk factors for cervical cancer and pre-cancerous lesions in Rwanda

    OpenAIRE

    Makuza, Jean Damascène; Nsanzimana, Sabin; Muhimpundu, Marie Aimee; Pace, Lydia Eleanor; Ntaganira, Joseph; Riedel, David James

    2015-01-01

    Introduction Cervical cancer prevalence in Rwanda has not been well-described. Visual inspection with acetic acid or Lugol solution has been shown to be effective for cervical cancer screening in low resource settings. The aim of the study is to understand the prevalence and risk factors for cervical cancer and pre- cancerous lesions among Rwandan women between 30 and 50 old undergoing screening. Methods This cross-sectional analytical study was done in 3 districts of Rwanda from October 2010...

  6. Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by (1)H NMR spectroscopy.

    Science.gov (United States)

    Hasim, Ayshamgul; Ali, Mayinuer; Mamtimin, Batur; Ma, Jun-Qi; Li, Qiao-Zhi; Abudula, Abulizi

    2012-06-01

    (1)H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one-way analysis of variance (ANOVA) test was used to examine the significance of the metabolites. Compared with plasma obtained from the healthy controls, plasma from patients with CIN had higher levels of very-low density lipoprotein (VLDL), acetone, unsaturated lipid and carnitine, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, glycine, acetylcysteine, myo-inositol, choline and glycoprotein. Plasma from patients with CSCC had higher levels of acetate and formate, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine and tyrosine compared with the plasma of the healthy controls. In addition, compared with the plasma of patients with CIN, the plasma of CSCC patients had higher levels of acetate, formate, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, tyrosine, acetylcysteine, myo-inositol, glycoprotein, α-glucose and β-glucose, together with lower levels of acetone, unsaturated lipid and carnitine. Moreover, the profiles showed high feasibility and specificity by statistical analysis with OPLS-DA compared to the Thinprep cytology test (TCT) by setting the histopathological outcome as standard. The metabolic profile obtained for cervical cancer is significant, even for the precancerous disease. This suggests a systemic metabolic response to cancer, which may be used to identify potential early diagnostic biomarkers of the cancer and to establish

  7. Electrocautery for Precancerous Anal Lesions

    Science.gov (United States)

    Results from a randomized clinical trial conducted in Amsterdam suggest that electrocautery is better than topical imiquimod or fluorouracil at treating potentially precancerous anal lesions in HIV-positive men who have sex with men.

  8. Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy

    OpenAIRE

    Hasim, Ayshamgul; ALI, MAYINUER; MAMTIMIN, BATUR; Ma, Jun-Qi; Li, Qiao-Zhi; ABUDULA, ABULIZI

    2012-01-01

    1H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one-way a...

  9. Trends in the incidence of cervical cancer and severe precancerous lesions in Denmark, 1997-2012

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Munk, Christian; Nielsen, Thor Schütt Svane;

    2015-01-01

    papillomavirus (HPV) vaccination. METHODS: Using nationwide registries, we estimated age-specific and age-standardized incidence rates and estimated annual percentage change (EAPC). RESULTS: The incidence of SCC decreased significantly, especially in women aged ≥45 years [EAPC: -3.1 % (95 % CI -4.3 to -2...... or the incidence of adenocarcinoma, which is increasing. Decreases in the incidences of CIN3 and AIS in age groups with high HPV vaccine coverage may herald a future decrease in cervical cancer incidence in young Danish women....

  10. Methylation and expression of miRNAs in precancerous lesions and cervical cancer with HPV16 infection.

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    Jiménez-Wences, Hilda; Martínez-Carrillo, Dinorah Nashely; Peralta-Zaragoza, Oscar; Campos-Viguri, Gabriela Elizabeth; Hernández-Sotelo, Daniel; Jiménez-López, Marco Antonio; Muñoz-Camacho, José Guadalupe; Garzón-Barrientos, Víctor Hugo; Illades-Aguiar, Berenice; Fernández-Tilapa, Gloria

    2016-04-01

    Abnormal expression and promoter methylation of microRNAs (miRNAs) are common events during cervical carcinogenesis. Worldwide, infection by types 18 and 16 of human papillomaviruses (HPVs) is considered the major risk factor for cervical cancer development. It has been reported that expression of the miRNAs can be deregulated by specific HPV genotypes. In this study we analyzed the promoter methylation of 22 miRNAs and the expression of three miRNAs in 10 non-squamous intraepithelial lesions (Non-SIL) without HPV16 infection, and 7 Non-SIL, 16 low-grade SIL (LSIL) and 16 cervical cancer samples, all with HPV16 infection. The methylation status was determined using Human Cancer miRNA EpiTect Methyl II Signature PCR Array® and the expression of miR-124, miR-218 and miR-193b was determined by qRT-PCR using individual TaqMan assays. Comparisons of groups defined were performed using the Fisher exact test for categorical variables and Mann-Whitney test for continuous variables. A p-value of <0.05 was considered statistically significant. The methylation levels of miR-124-2, miR-218-1, miR-218-2 and miR-34b/c promoters were significantly higher in cervical cancer than in LSIL samples. The methylation levels of miR-193b promoter were significantly lower in cervical cancer than in LSIL samples. The expression of miR-124 and miR-218 was significantly lower in cervical cancer than in LSIL samples. The expression of miR-193b was significantly higher in cervical cancer than in LSIL and Non-SIL samples. Our results suggest that the abnormal promoter methylation and expression of miR-124, miR-218 and miR-193b are common events during cervical carcinogenesis. PMID:26797462

  11. Study to assess the acceptability and feasibility of cervical cancer screening using visual inspection with acetic acid and treatment of precancerous lesions using cryotherapy in low resource settings

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    Tosha M. Sheth

    2014-06-01

    Full Text Available Background: Objective of current study was to assess the acceptability and feasibility of cervical cancer screening using VIA, and treatment of precancerous lesions using cryotherapy in low resource settings Methods: 526 women from three primary health centers of Shinor taluka (population based approach and 250 women of Medical College, Vadodara (facility based approach were sensitized and screened for cervical cancer. Visual Inspection with Acetic acid (VIA was performed as the screening test. In the population based approach, VIA positive women were referred to a Community Health Centre (CHC for colposcopy and biopsy. Ablative treatment in the form of cryotherapy was offered. Patients requiring higher forms of treatment were referred to medical college, Vadodara. In the facility based approach, VIA positive women underwent colposcopy. Guided biopsy was performed in those with positive lesions on colposcopy. Cryotherapy was offered in the same sitting. Those not suitable for cryotherapy were offered loop electrosurgical excision procedure. Women found to have invasive cancer were offered definitive management. Results: VIA positivity rate was 18.8% in the population based approach and 27.2% in the facility based approach.58.8% women in the population based approach and 77.77% women in the facility based approach were treated with cryotherapy on the same day as screening and none reported any severe side effects. Dropout rate in the community approach was 32.32% whereas in the facility it was 0.4%. Conclusions: VIA and cryotherapy procedures were well tolerated by all screened women. This project has shown that the and ldquo;screen and treat and rdquo; approach can be successfully implemented in the existing health setup. [Int J Reprod Contracept Obstet Gynecol 2014; 3(3.000: 615-621

  12. 宫颈刷片联合阴道镜活检对宫颈癌前病变的筛查分析%Analysis of cervical brush tablets combined with biopsy under colposcopy for screening cervical precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    李迎芳

    2016-01-01

    目的:探讨宫颈刷片联合阴道镜活检对宫颈癌前病变中的筛查作用。方法:选取自愿参加筛查的妇女410例,对其进行宫颈刷片联合阴道镜活检,对有数据进行统计分析。结果:阴道镜下活检组织相符率85.36%。阴道检查结果:CIN76例,CIN Ⅰ级43例,CIN Ⅱ级18例,CIN Ⅲ级9例,癌6例。与病理检查符合率55.26%。结论:宫颈刷片检查联合阴道镜活检能够更有效筛查宫颈癌前变患者,两项联合能够提高宫颈癌早期的诊断率。%Objective:To investigate the effect of cervical brush tablets combined with biopsy under colposcopy for screening cervical precancerous lesions.Methods:410 cases of voluntary screening were selected.All the patients underwentervical brush tablets combined with biopsy under colposcopy.We performed statistical analysis of all data.Results:The colposcopy biopsy conformity rate was 85.36%.Vaginal examination results:76 cases of CIN,CIN Ⅰ in 43 cases,CIN Ⅱ in 18 cases,9 cases of CINⅢ,6 cases of carcinoma.The coincidence rate with pathological diagnosis was 55.26% .Conclusion:The cervical brush tablets combined with biopsy under colposcopy can be more effective in screening of cervical precancerous patients,which can improve the early diagnosis rate of cervical cancer.

  13. 薄层液基细胞学在宫颈癌及其癌前病变筛查中的价值%The value of Thin prep cytology test in cervical precancerous lesions and cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    雷静; 万霖; 李惠新; 蒋瑛; 李瑞萍

    2011-01-01

    Objective: To evaluate the detecting technology of thin layers of liquid base cytology (TCT) on the diagnosis of precancerous lesions of uterine cervix and the clinical value, accuracy of the cervical cancer screening. Methods: To collect and analysis 7340 patients who were did examine of TCT in gynecological clinic line of our hospital from May, 2009 ~ November, 2010. Take cytology diagnosed ASC - US and higher as positive results, and the positive results do pathohistological. Take histological diagnosis as gold standard. Results: Thin prep cytology test specimens of SCC, and satisfaction LSIL accuracy HSIL, respectively, 76.8% 97.3%, 100%. Conclusion: TCT combination diagnostic systems of TBS are ideal method of current diagnosis precancerous lesions of uterine cervix (CIN) and cervical cancer screening. Also it can be used as an indicator for cervical cancer detecting. There is some risk of precancerous lesions of young In ASC-US patients.%目的:评价薄层液基细胞学(Thin prep cytology test,TCT)检测技术对宫颈癌前病变的诊断和宫颈癌筛查的准确性及临床价值.方法:收集分析2009年5月~2010年11月在我院妇科门诊行TCT检查的受检者7340例,以细胞学诊断为未明确意义的不典型鳞状上皮细胞(ASC-US)及以上者为阳性结果,并对阳性结果行病理组织学诊断,以组织学诊断作为金标准.结果:液基细胞学标本满意度高,对SCC、HSIL、LSIL的准确率分别为76.8%、97.3%、100%.结论:TCT结合TBS诊断系统是目前诊断宫颈癌前病变和筛查宫颈癌的理想方法 [1],同时也可以作为一项宫颈癌术后随访的检测指标 [2].ASC-US患者中存在部分年轻的高危癌前病变者.

  14. Risk of Preterm Delivery Associated with Prior Treatment of Cervical Precancerous Lesion according to the Depth of the Cone

    Science.gov (United States)

    Berretta, Roberto; Gizzo, Salvatore; Dall'Asta, Andrea; Mazzone, Eleonora; Monica, Michela; Franchi, Laura; Peri, Francesca; Patrelli, Tito Silvio; Bacchi Modena, Alberto

    2013-01-01

    The aim of this study was to evaluate the impact of the surgical excisional procedures for cervical intraepithelial neoplasia (CIN) treatment both on subsequent fertility (cervical factor) and pregnancy complication (risk of spontaneous preterm delivery). We retrospectively analyzed 236 fertile women who underwent conization for CIN. We included in the study 47 patients who carried on pregnancy and delivered a viable fetus. Patients were asked about postconization pregnancies, obstetrical outcomes, and a possible diagnosis of secondary infertility caused by cervical stenosis. We evaluated the depth of surgical excision, the timing between cervical conization and subsequent pregnancies, surgical technique, and maternal age at delivery. We recorded 47 deliveries, 10 cases of preterm delivery; 8 of them were spontaneous. The depth of surgical excision showed a statistically significant inverse correlation with gestational age at birth. The risk of spontaneous preterm delivery increased when conization depth exceeded a cut-off value of 1.5 cm. Our data do not demonstrated a relation between conization and infertility due to cervical stenosis. PMID:24324288

  15. ANALYSIS OF COLPOSCOPY EXAMINATION FOR THE CERVICAL CANER AND ITS PRECANCEROUS LESIONS%阴道镜检查在宫颈癌及癌前病变筛查中的分析

    Institute of Scientific and Technical Information of China (English)

    梅天琼; 李静

    2011-01-01

    [Objective] To analyze the effect of colposcopy examination for the cervical caner and its precancerous lesions. [Methods] From June 2009 to December 2011, 835 cases of cervical intraepithelial pathological change, were detected by colposcope and results were compared with biopsy pathology. [Results] The accordance rate of the colposcopy examination and pathology diagnosis diagnosed cervicitis, C1N I , CIN II , CIN HI and SCC were 59.7%, 62.7%, 74.0%, 63.9% and 81.8%. [Conclusion] Colposcopy examination in the diagnosis of cervical disease is more reliable, and with higher sensitivity for cervical cancer, which is valuable for examining cervical cancer and precancerous lesions.%[目的]分析阴道镜检查在宫颈癌及癌前病变筛查中的效果.[方法]2009年6月~2010年12月,将835例阴道镜检查异常者阴道镜检查结果与病理学检查诊断结果间进行对比分析.[结果]阴道镜检查为炎症、CINⅠ、CINⅡ、CINⅢ和SCC者与病理诊断结果比较,诊断符合率分别为59.7%、62.7%、74.0%、63.9%和81.8%.[结论]阴道镜下检查对宫颈疾病的诊断比较可靠,对宫颈癌的敏感性较高,对宫颈癌及癌前病变检查具有较高的临床价值.

  16. Correlation of human papilloma virus presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry

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    Meenu Pujani

    2012-01-01

    Full Text Available Background : Cancer of cervix is the most common form of cancer in females of developing countries. Cervical cancer is the best example of common human malignancy with a proven infectious etiology. The data linking human papilloma virus (HPV infection with the epidemiology and pathogenesis of cervical neoplasia is convincing. There are various methods for detection of HPV like immunohistochemistry, polymerase chain reaction, liquid phase hybridization (hybrid capture test, in situ hybridization etc. Materials and Methods: We studied the profile of precancerous and cancerous lesions of uterine cervix and correlated human papilloma virus (HPV presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry. Total 50 cases were first studied, analyzed, and classified histologically and then immunohistochemistry was done. Results : Majority of the cases 36/50 (72% under study are squamous cell carcinomas, which is the most common carcinoma found in the uterine cervix. HPV positivity for all cases of cervical carcinoma was found to be 34.7% (16/46 cases, for carcinoma in situ, it was 50% (2/4 cases. It can be thus hypothesized that with decreasing differentiation, there is loss of expression of HPV in the cervical epithelial cells. Conclusion: To conclude, though this study confirms the correlation of HPV presence with precancerous and cancerous lesions of uterine cervix, it also suggests that there must be other co-factors involved in cervical carcinogenesis as well.

  17. Study on the relationship between high risk human papilloma virus infection and cervical cancer and precancerous lesions%高危人乳头瘤病毒感染与宫颈癌及癌前病变的关系探讨

    Institute of Scientific and Technical Information of China (English)

    余锦芬

    2012-01-01

    目的 探讨人乳头瘤病毒(HPV)感染与宫颈癌、癌前病变的关系及不同亚型HPV病毒重叠感染对宫颈癌、癌前病变的影响.方法 以148例宫颈癌及癌前病变患者为观察组并以96例宫颈炎患者为对照组,观察两组研究对象高危HPV病毒感染率、多重感染对宫颈癌、癌前病变的影响及不同病理类型宫颈癌高危HPV感染率.结果 HPV病毒感染率以鳞癌、腺癌居多;高危型HPV病毒感染率、双重感染率、多重感染率在宫颈癌中较高,CINⅢ期、CINⅡ期、CIN Ⅰ期依次降低,均高于宫颈炎中感染率.结论 高危型HPV病毒感染与宫颈癌的发生、发展密切相关,阻断高危型HPV病毒的持续感染可阻断癌前病变的进程,是防治宫颈癌的关键所在.%OBJECTIVE To explore the relationship between human papilloma virus (HPV) infection and cervical carcinoma, precancerous lesion and study the effects of different subtypes of HPV virus superinfection on cervical cancer, precancerous lesion. METHODS 148 cases of cervical cancer and precancerous lesions of patients in observation group, and 96 cases of cer-vicitis patients as control group, observed high risk HPV infection rate, the effect of multiple infection on cervical cancer and precancerous lesions, and high risk HPV infection rate of different pathological types of cervical cancer in subjects of two groups. RESULTS HPV virus infection rates were more in squamous cell carcinoma, adenocarcinoma; high-risk HPV infection rates, double infection rates, multiple infection rates was higher in cervical cancer, and in stage Ⅲ , CIN, CIN Ⅱ , CIN Ⅰ lowered in turn, they were higher than in the infection rate of cervicitis. CONCLUSION The high risk HPV virus infection is closely associated with occurrence and development of cervical cancer. Blocking high risk HPV virus persistent infection can block precancerous lesions of the process, it's the key to prevent and treat cervical cancer.

  18. Treatment of gastric precancerous lesions with Weiansan

    Institute of Scientific and Technical Information of China (English)

    Hui-Zhen Li; Qiang Gao; Hong Wang; Guan-Qun Wang; Jie Liu; Shuang-Mei Zhao; Jie Chen; Qing-Wu Song; Wang Gao; Xiang-Zheng Qi

    2006-01-01

    AIM: To observe the curative effect of Weiansan (WAS)on gastric precancerous lesions (GPL) and H pylori elimination.METHODS: Seventy-six patients with GPL were randomly divided into two groups: WAS group (n = 42)and Weifuchun (WFC) group (n = 34). The patients in the WAS group were administered 5 g WAS 3 times a day, and the patients in the WFC group took WFC (4tablets) 3 times a day. To monitor inflammation of gastric mucosa, degree of glandular atrophy (GA), intestinal metaplasia (IM) and dysplasia, and H pylori infection,all patients underwent gastroscopy and biopsy with pathological examination before and after treatment. Fifty male Sprague-Dawley (SD) rats were used in animal experiments. Of these, 10 served as the control group (n = 10), 40 were given ranitidine combined with N-methylN1-nitro-N-nitrosoguanidine (MNNG) for 12 wk and divided into 4 groups randomly: model group (n = 10),high-dose WAS group (n = 10), low-dose WAS group (n = 10) and WFC group (n = 10). Twelve weeks later,all rats were killed and a 2 cm x 1 cm tissue was taken from the lesser curvature of the gastric antrum.H pyloriinfection was determined by the fast urease method.RESULTS: The curative effect in WAS groups was similar to that in WFC groups. There was no statistical difference in degree of GA, IM and dysplasia between WAS and WFC groups. The rate of H pylori infection in the model group (positive/negative: 9/1) was significantly higher than that in the control group (positive/negative: 1/9)(P < 0.01). H pylori elimination in the high-dose WAS group (positive/negative: 4/6) and low-dose WAS group (positive/negative: 6/4) was similar to that in the WFC group (positive/negative: 4/6) (P > 0.05).CONCLUSION: WAS improves clinical symptoms by suppressing GA, IM and dysplasia and eliminating H pylori.

  19. Association between selected oral pathogens and gastric precancerous lesions.

    Directory of Open Access Journals (Sweden)

    Christian R Salazar

    Full Text Available We examined whether colonization of selected oral pathogens is associated with gastric precancerous lesions in a cross-sectional study. A total of 119 participants were included, of which 37 were cases of chronic atrophic gastritis, intestinal metaplasia, or dysplasia. An oral examination was performed to measure periodontal indices. Plaque and saliva samples were tested with real-time quantitative PCR for DNA levels of pathogens related to periodontal disease (Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, Actinobacillus actinomycetemcomitans and dental caries (Streptococcus mutans and S. sobrinus. There were no consistent associations between DNA levels of selected bacterial species and gastric precancerous lesions, although an elevated but non-significant odds ratio (OR for gastric precancerous lesions was observed in relation to increasing colonization of A. actinomycetemcomitans (OR = 1.36 for one standard deviation increase, 95% Confidence Interval = 0.87-2.12, P. gingivalis (OR = 1.12, 0.67-1.88 and T. denticola (OR = 1.34, 0.83-2.12 measured in plaque. To assess the influence of specific long-term infection, stratified analyses by levels of periodontal indices were conducted. A. actinomycetemcomitans was significantly associated with gastric precancerous lesions (OR = 2.51, 1.13-5.56 among those with ≥ median of percent tooth sites with PD ≥ 3 mm, compared with no association among those below the median (OR = 0.86, 0.43-1.72. A significantly stronger relationship was observed between the cumulative bacterial burden score of periodontal disease-related pathogens and gastric precancerous lesions among those with higher versus lower levels of periodontal disease indices (p-values for interactions: 0.03-0.06. Among individuals with periodontal disease, high levels of colonization of periodontal pathogens are associated with an increased risk of gastric precancerous lesions.

  20. A model of gastric precancerous lesions for rats

    Institute of Scientific and Technical Information of China (English)

    Mao Xiang Yan; Zhi Yun Chen; Bai Kang Xiang

    2000-01-01

    AIM To establish an ideal model of gastric precancerous lesions for rats.METHODS Fifty rats were fed with carcinogen MNNG for 35 wk and heat-plaste at 60C for 20 wk, thepathological changes were observed. Data in the groups were analyzed by Ridit test.RESULTS The occurrence rate of dysplasia in rats due to MNNG and heat-damage was 65%, which wasobviously higher than that due to MNNG only. The differences between the two groups were significant.CONCLUSION The animal model made by MNNG and heat-damage was an ideal one which could be usedto investigate the pathologic mechanism of gastric precancerous lesions.

  1. [Precancerous and cancerous lesions of the oral cavity].

    Science.gov (United States)

    Loeb, I; Evrard, L

    2008-09-01

    Precancerous states of the oral mucosa refers to pathologies in which there is a risk of malignancy development, compared to normal mucosa. Some histological alterations characteristic of precancerous lesions can be easily detected in a biopsy of the lesion, allowing their classification, and hence adequate treatment. A systematic examination of the oral mucosa in patients is recommended, especially in patients who show risk factors such as tabagism and/or alcohol consumption, in order to contribute to a decrease in the incidence of malignant tumor of the oral cavity. PMID:18949975

  2. PRECANCEROUS AND CANCEROUS LESIONS OF CERVIX DIAGNOSED BY PAP’S SMEAR - A HOSPITAL BASED STUDY

    Directory of Open Access Journals (Sweden)

    Komal Singh

    2014-02-01

    Full Text Available BACKGROUND: Cervical intra - epithelial neoplasia (CIN and cervical cancer remain important health problems for women worldwide. It is largely a preventable disease that is characterized by a long lead time. The objective of this study was to determine the frequency of precancerous lesions of cervix by Pap smear screening. METHODS : A retrospective hospital based study was conducted at Department of Pathology, in a Tertiary Care Institute and associated hospital, Bhopal. Data was collected from the records of Pap’s smear testing done on 388 patients in years 2011 & 2012. Analysis was done by entering data in SPSS software and Chi - square test was applied to find statistical significance. RESULTS: Most common lesions on Pap’s smear was Low grade squamous intraepithelial les ion (LSIL in 104 (26.8% of cases and High grade squamous intraepithelial lesion (HSIL in 15 (3.9% of cases. 44 (11.3% were senile Atopic Changes, parasitic Infection in 27 (7% of cases and 2 (0.5% were carcinoma cervix cases. All the cervical lesion s were most common in age of 31 - 40 yrs. and were significantly associated with age. CONCLUSIONS: Pap’s smear examination information should be spread in community apart from hospitals. Especially the young women should be motivated for regular Pap’s smear. Pap’s smear is the single most important screening method for early detection of precancerous & cancerous cervical lesions

  3. 阴道镜联合宫颈锥切对宫颈癌及其癌前病变患者的诊断价值分析%Diagnostic Value of Colposcopy and Cervical Conization for Cervical Cancer and Precancerous Lesion

    Institute of Scientific and Technical Information of China (English)

    易黎; 归倩

    2014-01-01

    Objective To explore the diagnostic value of colposcopy and cervical conization for cervical cancer and pre -cancerous lesion .Methods 618 cases with cervical cancer and precancerous lesion were selected as research subjects ,including 32 cases of CINⅠ,58 cases of CINⅡ,40 cases of CIN Ⅲ and 8 cases of cervical cancer .Results The sensitivity,specificity, positive predictive value and negative predictive value of colposcopy for cervical cancer and precancerous lesion were 74.6%, 93.8%,77.4%and 92.8%,respectively;The rates of correct diagnosis of colposcopy for CIN Ⅰ,CIN Ⅱ and CIN Ⅲ were 59.4%(19 cases),79.3%(46 cases) and 82.5%(33 cases);22 cases of CINⅠ,50 cases of CINⅡand 36 cases of CIN Ⅲwere detected by cervical conization;5 cases (62.5%) and 2 cases(25.0%) of cervical cancer were detected by colposcopy or cervical conization respectively .Combined diagnostic rate of colposcopy and cervical conization was 87.5%.Conclusion Colpos-copy and cervical conization improve the diagnostic rate of cervical cancer and precancerous lesion .%目的:探讨阴道镜联合宫颈锥切( LEEP)对宫颈癌及其癌前病变患者的诊断价值。方法选择618例宫颈癌及癌前病变患者为研究对象,其中病理诊断确诊宫颈上皮内瘤变( CIN)Ⅰ级32例,Ⅱ级58例,Ⅲ级40例,宫颈浸润癌8例。采用宫颈锥切治疗,分析手术前后病理诊断情况。结果在618例患者中,阴道镜诊断宫颈癌及癌前病变的灵敏度、特异度、阳性预测值及阴性预测值分别为74.6%、93.8%、77.4%和92.8%;阴道镜正确诊断CINⅠ级、CINⅡ级及CINⅢ级分别为19例(59.4%)、46例(79.3%)及33例(82.5%);子宫锥切术检出CINⅠ级22例,Ⅱ级50例,Ⅲ级36例;阴道镜诊断宫颈浸润癌5例(62.5%),经宫颈锥切另发现2例(25.0%),两者联合诊断宫颈浸润癌准确率为87.5%。结论阴道镜联合宫颈锥切可

  4. CCR2-V64I polymorphism is associated with increased risk of cervical cancer but not with HPV infection or pre-cancerous lesions in African women

    International Nuclear Information System (INIS)

    Cervical cancer, caused by specific oncogenic types of human papillomavirus (HPV), is the second most common cancer in women worldwide. A large number of young sexually active women get infected by HPV but only a small fraction of them have persistent infection and develop cervical cancer pointing to co- factors including host genetics that might play a role in outcome of the HPV infection. This study investigated the role of CCR2-V64I polymorphism in cervical cancer, pre-cancers and HPV infection in South African women resident in Western Cape. CCR2-V64I polymorphism has been previously reported to influence the progression to cervical cancer in some populations and has also been associated with decreased progression from HIV infection to AIDS. Genotyping for CCR2-V64I was done by PCR-SSP in a case-control study of 446 women (106 black African and 340 mixed-ancestry) with histologically confirmed invasive cervical cancer and 1432 controls (322 black African and 1110 mixed-ancestry) group-matched (1:3) by age, ethnicity and domicile status. In the control women HPV was detected using the Digene Hybrid Capture II test and cervical disease was detected by cervical cytology. The CCR2-64I variant was significantly associated with cervical cancer when cases were compared to the control group (P = 0.001). Further analysis comparing selected groups within the controls showed that individuals with abnormal cytology and high grade squamous intraepitleial neoplasia (HSIL) did not have this association when compared to women with normal cytology. HPV infection also showed no association with CCR2-64I variant. Comparing SIL positive controls with the cases showed a significant association of CCR2-64I variant (P = 0.001) with cervical cancer. This is the first study of the role of CCR2-V64I polymorphism in cervical cancer in an African population. Our results show that CCR2-64I variant is associated with the risk of cervical cancer but does not affect the susceptibility to HPV

  5. The effect of human papillomavirus DNA testing and cervical cytology for screening of cervical precancerous lesions%人乳头瘤病毒DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果

    Institute of Scientific and Technical Information of China (English)

    梁瑜; 彭华丽

    2015-01-01

    Objective To investigate the effect of human papillomavirus (HPV) DNA testing and cervical cytology screening of cervical precancerous lesions. Methods 1100 patients admitted to our hospital who were carried out screening cervical precancerous lesions from January 2013 to June 2014 were selected,all were carried out HPV DNA testing,cervical cytology,colposcopy and cervical biopsy,pathological diagnosis was as the standard,the test results were analyzed. Results Positive rate of HPV DNA testing was 23.00% in 1100 patients,in which,positive rate of cervical cancer was 83.33%,positive rate of cervical intraepithelial neoplasia (CIN) Ⅰ was 44.44%,CIN II testing was 86.67%, CINⅢ positive rate was 94.12%.Cervical cytology results showed that there were 851 cases (77.36%) with no CIN,139 cases (12.64%) were atypical squamous cells,78 cases (7.09%) were low-grade squamous intraepithelial lesion,29 cases (2.64%) of high-grade squamous intraepithelial cells lesions,3 cases (0.27%) of squamous cell carcinoma.The compli-ance rate of colposcopy examination and pathological diagnosis was 62.79% (54/86). Conclusion The suitable cervical lesions screening method for patients can help to improve the detection rate,reduce the incidence rate of cervical cancer.%目的:探讨人乳头瘤病毒(HPV)DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果。方法选取2013年1月~2014年6月来本院就诊并进行宫颈癌前病变筛查的1100例患者,均进行HPV DNA检测、宫颈细胞学检查、阴道镜检查与宫颈活检,以病理诊断为标准,对检测结果进行分析。结果1100例患者中,HPV DNA阳性率为23.00豫,其中宫颈癌阳性率为83.33豫,宫颈上皮内瘤变(CIN)Ⅰ阳性率为44.44豫,CINⅡ阳性率为86.67豫,CINⅢ阳性率为94.12豫。宫颈细胞学检查无CIN 851例(77.36豫),非典型鳞状上皮细胞139例(12.64豫),低度鳞状上皮细胞内病变78例(7.09豫),高度鳞状上皮细胞内病变29

  6. The role of HPV genotyping test and liquid-based cytology in diagnosis of cervical pre-cancerous lesions%人乳头瘤病毒分型基因检测和液基细胞学检查在宫颈癌前病变中的诊断作用

    Institute of Scientific and Technical Information of China (English)

    谢芳; 黎云; 彭秀红; 李洁明

    2011-01-01

    Objective To evaluate the role and significance of HPV genotyping detection and Thinprep cytology test (TCT ) in the diagnosis of cervical precancerous lesions ( mild to moderate epithelial neoplasia ). Methods The findings of HPV genotyping and TCT were analyzed in 401 patients with cervical precancerous lesions ( CIN1-CIN2 ) who had been hospitalized between November 2008 to November 2010. Results The sensitivity of HPV genotyping was significantly higher than that of TCT ( P<0.01 ).TCT combined with HPV genotyping enhanced the detection rate of cervical lesions. Subtypes 16, 52, 58, and 18 of HPV were common in high-risk HPV infection with cervical precancerous lesions. Subtype 16 had a markedly higher rate of occurrence of cervical lesions than subtypes 52 and 58 ( P < 0.01 ). Conclusions High-risk HPV infection is the major pathogenic factor for cervical cancer and precancerous lesions. HPV 16 infection easily leads to cervical lesion. HPV genotyping test combined with Thinprep cytology test can increase the detection rate of cervical precancerous lesions.%目的 评估人乳头瘤病毒(HPV)基因分型检测和液基细胞学检查(TCT)在宫颈轻、中度上皮细胞内瘤变(CIN1-CIN2)中的诊断作用及意义.方法 对我院2008年11月-2010年11月期间401例因宫颈癌前病变(CIN1-CIN2)要求官颈环切的患者的HPV分型基因检测情况与液基细胞学检查结果进行总结分析.结果 HPV的灵敏性较TCT明显增高(P<0.01),TCT和HPV基因分型检测结合能提高宫颈病变的检出率;宫颈癌前病变中HPV感染以高危基因组的16、52、58、18亚型感染多见,HPV16犁感染引起高度病变的几率较HPV52型和HPV58型明显增高(P<0.01).结论 高危HPV感染是宫颈癌及癌前病变发病的主要致病因子,HPV16型感染易导致宫颈高度病变;HPV基因分型检测和液基细胞学检查结合,可以提高宫颈癌前病变的检出率.

  7. Research Progress in Correlation of Vaginal Lactobacillus with HPV Infection, Cervical Cancer and Precancerous Lesions%阴道乳酸杆菌与HPV感染、宫颈癌及癌前病变的相关性研究进展

    Institute of Scientific and Technical Information of China (English)

    王颖; 刘植华

    2013-01-01

    乳酸杆菌及其代谢产物具有抑制肿瘤的作用,通过产生各种代谢产物干扰肿瘤代谢,以及激活机体免疫功能而发挥作用.乳酸杆菌在防治各种肿瘤中的作用也已引起重视.阴道乳酸杆菌是构成阴道微环境的重要因素,其数量的减少与HPV感染、宫颈癌前病变及宫颈癌有关.文章就阴道乳酸杆菌与HPV感染、宫颈癌及癌前病变的关系进行综述.%Previous studies had demonstrated that lactobacillus had antitumor effect.It interferences tumor metabolism through the production of metabolites and activates the immune function.Effect of lactobacillus in the prevention and treatment for various tumors has aroused attention.Lactobacillus is a dominated factor in normal women's vaginal environment.Decreasing of vaginal lactobacillus related to human papilloma virus (HPV) infection,cervical cancer and precancerous lesions.The relationship of lactobacillus with HPV infection,cervical cancer and cervical precancerous lesions is reviewed.

  8. Evaluation of computer-assisted ThinPrep imaging system in the screening of cervical cancer and precancerous lesion%TIS在宫颈癌和癌前病变筛查中的价值评价

    Institute of Scientific and Technical Information of China (English)

    米贤军; 王莹; 沈铿; 吴秋良; 肖琳; 陈昂; 徐秀梅; 孪峰; 钟守军

    2013-01-01

    目的 评价新柏氏电脑辅助阅片系统(TIS)对宫颈癌及癌前病变的诊断价值.方法 收集2011年6月至2012年6月参加中山市大规模免费宫颈癌筛查中妇女的资料,选择做过TIS检测并追踪到有确切组织病理学诊断结果的600例病例为研究对象,将ASCUS/AGC+病变列为细胞学阳性病例,将CIN1+病变列为病理学阳性病例,以病理学检查为金标准,评价TIS检测的诊断价值.结果 600例妇女中,TIS检测结果为癌(CA)9例,高度鳞状上皮内病变(HSIL) 66例,低度鳞状上皮内病变(LSIL) 104例,不除外高度鳞状上皮内病变的不典型鳞状细胞(ASC-H) 21例,非典型鳞状细胞/非典型腺细胞(ASCUS/AGC) 30例,正常(WNL) 370例.以病理学诊断结果为金标准,TIS检测的灵敏度为93.95%,特异度为92.73%,约登指数为0.867,阳性似然比12.92,阴性似然比0.065,Kappa值0.854,总符合率为93.17%,阳性预测值87.83%,阴性预测值96.49%.TIS与病理学结果的符合率在CA、HSIL、LSIL中分别为100%、90.91%和72.12%,TIS对CA和HSIL的符合率高于LSIL (P<0.01).结论 TIS对宫颈癌及癌前病变有较高的诊断价值,特别是对CA和HSIL.它是一种高效、高质量和高准确率的宫颈癌及癌前病变筛查技术.%Objective To study the diagnostic value of computer-assisted ThinPrep imaging system(TIS) in the screening of cervical cancer and precancerous lesion.Methods The clinical data of 600 cases who participated voluntarily in the screening of large-scale cervical cancer for free in Zhongshan were collected from June 2011 to June 2012.ASCUS/AGC and above were postive cytology and the postive results of pathological examination were CIN and above.Histopathological results were used as gold standard to calculate the diagnostic value of TIS technique.Results A total of 600 cases were detected by TIS,and the testing results showed 9 cases of CA,66 cases of HSIL,104 cases of LSIL,21 cases of ASC-H,30 cases of ASCUS

  9. 叶酸与DNA甲基转移酶1在宫颈癌及癌前病变中的作用%Effect of folic acid and DNA methyltransferase 1 on cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    王金桃; 霍晓旭; 丁玲; 郝敏; 樊金荣; 张丽; 赵维敏

    2011-01-01

    Objective To explore the effect of folic acid and DNA methyltransferase 1 (DNMT1) on cervical cancer and cervix precancerous lesion. Methods 100 patients with cervix squamouscell carcinoma (SCC), 101 patients with cervical intraepithelial neoplasm (CIN) and 109 patients with cervix inflammation (CI) diagnosed by histology were included in this study. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and Western blot were used to detect the levels of serum folate, HPV16 infection and the expression of DNMT1 protein,respectively. Results The average levels of serum folate were (2.60 ± 1.61) ng/ml, (3.14 + 2.08) ng/ml and (3.32+1.74) ng/ml,and the expression of DNMT1 protein were 2.40 + 0.99,1.88 + 0.33 and 0.89 ± 0.29 in the group of SCC, CIN and CI, respectively.The relationship of folate levels and DNMT1 protein expression showed inverse correlation (r=-0.186, P=0.00l). The results in our study indicated that there was an additive interaction between low-level of serum folate and high-expressionof DNMT1 protein related to the risk of CIN and SCC, with OR value as 2.50(95%C/: 1.21-9.22) and 6.03 (95%C/: 2.79-21.72) respectively. The relative excessrisk of interaction (RERI) , attributableproportion of interaction (API) and synergy index (S) were 0.92, 0.36 and 2.59 in the CIN group while 2.47, 0.41 and 1.96 in the SCC group. Conclusion The low level of serum folate and high expression of DNMT1 protein seemed to be associated with high risk of cervical cancer and its precancerous lesion. It suggested that there might be a synergistic action between serum folate and DNMT1 in the progression of cervix carcinogenesis.%目的 探讨叶酸和DNA甲基转移酶1(DNMT1)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者100例、宫颈上皮内瘤样变(CrN)患者101例和宫颈炎(CI)患者109例为研究对象.在收集全部对象人口学特征、生殖因素等相关资料

  10. Effect of jianpiyiwei capsule on gastric precancerous lesions in rats

    Institute of Scientific and Technical Information of China (English)

    Xue-Ying Shi; Feng-Zhi Zhao; Xin Dai; Lian-Sheng Ma; Xiu-Yu Dong; Jie Fang

    2002-01-01

    AIM:To evaluate the therapeutic effect of compoundChinese drugs, Jianpiyiwei capsule (JPYW) on gastricprecancerous lesions in rats and to explore itsmechanism of action.METHODS:Model of gastric precancerous lesions wasconstructed in male Wistar rats: a metal spring wasinserted and fixed through pyloric sphincter. One weekafter recovery, each rat was given 50-60 ℃ hot pastecontainingt50 g/L NaCl 2 mL orally, twice a week for15 weeks.Then 10 normal and 11 model rats wereanaesthetized, after the measurement of gastricmucosa blood flow (GMBF), the rats were killed andthe mucosal hexosamines and malonic dialdehyde(MDA) were measured. The morphological changes ofgastric mucosa were observed macroscopically andmicroscopically, and by an automatic imaging analysissystem. Other rats were treated with JPYW 1.5 g/kg.d-1or 4.5 g/kg@d-1, or distilled water as negative controlrespectively (n=-10 in each group). After 12 weeks, allthe rats were examined as above.RESULTS: The gastric mucosa of model rats showedchronic atrophic gastritis with dysplasia and intestinalmetaplasia (IM), GMBF and hexosamine content werereduced significantly and MDA was increased ascompared to the normal group (P<0.01). After 12 weekstreatment, the pathological changes of the negativecontrol group became worsened, while in JPYW treatedgroups the changes were modified with significantincrease of GMBF and reduction of MDA, although thehexosamine concentration increased only mildly.CONCLUSION: JPYW increases GMBF and reduces MDAcontent in gastric mucosa and has therapeutic effectson gastric precancerous lesions.

  11. Liquid Based Cytology and Colposcopy in the Screening of Cervical Cancer and Precancerous Lesions%宫颈液基细胞学及阴道镜在宫颈癌及癌前病变中的筛查作用

    Institute of Scientific and Technical Information of China (English)

    王清; 胡霞

    2015-01-01

    目的:分析液基细胞学和阴道镜在宫颈癌及癌前病变中的筛查作用,评估液基细胞学和阴道镜在宫颈癌及癌前病变筛查中的临床应用价值。方法回顾性分析2013年5月-2014年8月接受宫颈癌筛查的500例女性的临床资料,所有体检者都接受阴道镜检查和液基细胞学检查。结果阴道镜检测假阳性患者有11例,阴道镜检测的特异性为97.39%(412/423),漏诊率为1.00%(5/500);液基细胞学诊断的假阳性患者有15例,由检测结果可知液基细胞学检测宫颈癌的检测特异性为96.45%(408/423),漏诊率为6.40%(32/500)。结论液基细胞学和阴道镜在宫颈癌及癌前病变筛查中具有临床应用价值,阴道镜对宫颈癌及癌前病变的检出率较液基细胞学高。%Objective To study the application value of liquid based cytology and colposcopy in the screening of cervical cancer and precancerous lesions .Methods Retrospective analysis of clinical data of 500 female patients who underwent cervical cancer screening were conducted .All subjects received colposcopy and liquid based cytology .Results Colposcopy results showed that false positive were 11 cases,the specificity of colposcopy was 97.39%(412 /423),the rate of missed diagnosis was 1.00%(5 /500);liquid based cytology diagnosis showed that false positive were 15 cases,specificity was 96.45%(408/423),the rate of missed diagnosis was 6.40%(32 /500).Conclusion Liquid based cytology and colposcopy has clinical application value in the screening of cervical cancer and precancerous lesions ,Colposcopy for cervical cancer and precancerous lesions has higher detec -tion rate compared with liquid based cytology .

  12. Study of the interaction of the risk factors in cervical cancer risk degree and the relationship between HPV genotype and cervical cancer and precancerous lesions%危险因素与宫颈癌发生危险度的交互作用及HPV基因型与宫颈癌及其癌前病变关系的研究

    Institute of Scientific and Technical Information of China (English)

    杨丽霞; 董剑梅

    2015-01-01

    目的:研究危险因素与宫颈癌发生危险度的交互作用及HPV基因型与宫颈癌及其癌前病变关系.方法:宫颈癌患者以及对照组共645例,对其相关资料给予回顾性分析.结果:性传播疾病史、性伴侣数、孕次是引发宫颈癌的危险因素,可是交互作用差异不具有统计学意义(P>0.05);感染以后 CIN Ⅰ危险性较高的 HPV 基因型为HPV-16,HPV-18,HPV-31,HPV-33,HPV-45,HPV-53,HPV-58,HPV-59.结论:宫颈癌行为危险因素与HPV 感染有联合作用.性传播疾病史以及性伴侣数的联合作用增加宫颈癌的发生风险.另外,HPV 感染当中, HPV-52是最为流行的亚型,其次为HPV-16,HPV-18,HPV-33,HPV-53.%Objective:To study the interaction of the risk factors in cervical cancer risk degree and the relationship between HPV genotype and cervical cancer and precancerous lesions.Methods:The patients with cervical cancer and the control group were 645 cases.The related data were given retrospective analysis.Results:The sexually transmitted diseases history,sexual partners number and gravidity were the risk factors for cervical cancer,but the difference of interaction was not statistically significant(P>0.05). The HPV genotypes with higher CIN Ⅰrisk degree after infection were HPV-16,HPV-18,HPV-31,HPV-33,HPV-45,HPV-53, HPV-58,HPV-59.Conclusion:The behavior risk factors of cervical cancer and HPV infection have a combined effect.The combined effect of sexually transmitted diseases history and sexual partners number can increase the occurrence risk of cervical cancer.In addition,in the HPV infection,HPV-52 is the most popular subtype,followed by HPV-16,HPV-18,HPV-33,HPV-53.

  13. Clinical Significance of HC2-HPV Assay Combined with Liquid Based Cytology in the Screen of Cervical Cancer and Precancerous Lesions%HC2-HPV 检测法联合液基细胞学在宫颈癌及癌前病变筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    徐艺; 杨静秀; 王玉平

    2016-01-01

    目的:探讨HC2-HPV检测法联合液基细胞学在筛查宫颈癌及癌前病变中的意义。方法应用HC2-HPV检测法联合液基细胞学技术对体检的患者进行免费宫颈癌及癌前病变的筛查。对不典型增生患者或(和) HC2-HPV检测阳性的患者行阴道镜下宫颈活检。结果液基细胞学检查对宫颈高度病变的检出率为11.0%(11/100);HC2-HPV检测对宫颈高度病变的检出率为27.2%(22/81),对宫颈癌的检出率高达100.0%。 HC2-HPV检测法联合液基细胞学检测阳性与病理检查阳性符合率为64.2%(52/81),显著优于TCT(52.0%)单独检查时的阳性符合率(P<0.05,χ2=11.39)。结论 HC2-HPV检测法联合液基细胞学检测对宫颈癌及癌前病变的筛查具有重要的临床推广价值。%Objective To investigate the significance of HC 2-HPV assay combined with liquid based cytology in the screen of cervical cancer and precancerous lesions .Methods HC2-HPV assay combined with liquid based cytology was used in the screen of cervical cancer and precancerous lesions .Cervical biopsy was performed in patients with atypical hyperplasia or (and) HC2-HPV positive patients.Results Detection rate of liquid based cytology for cervical lesion was 11.0%(11/100),and detection rates of HC2-HPV for cervical lesion and cervical cancer were 27.2%(22/81) and 100.0%.The coincidence rate of HC2-HPV combined with liquid based cytology and biopsy results was 64.2%(52/81),which was significantly higher than that of TCT(52.0%)(P<0.05,χ2 =11.39).Conclusion HC2-HPV assay combined with liquid based cytology test has important clinical value in the screen of cervical cancer and precancerous lesions .

  14. Clinical value of high-risk human papillomavirus DNA test,liquid-based cytology test in diagnosis of cervical cancer and precancerous lesion%高危型人乳头状瘤病毒DNA、液基薄层细胞检测在宫颈癌及前期病变诊断中的临床价值

    Institute of Scientific and Technical Information of China (English)

    杨秀珍

    2014-01-01

    Objective To investigate the clinical significance of high-risk human papillomavirus (HPV) DNA test com-bined with cytology inspection in the screening of cervical precancerous lesions. Methods 5080 women were selected from February 2013 to January 2014 in our hospital,they were given screen of cervical precancerous lesions.Cervical cytology,high-risk HPV DNA testing,colposcopy and cervical biopsy were done in them,and the results were analyzed with statistical methods. Results ASC-US (atypical squamous meaning is not clear epithelial cells) group of 318 cases, LSIL (low-grade squamous intraepithelial lesions) 26 cases of group,HSIL (high-grade squamous intraepithelial lesion) group of 52 cases,6 cases of invasive group confirmed by histopathological examination. Sensitivity and negative predic-tive value of HPV +TCT (liquid-based cytology test) to detect cervical lesions were higher than those high-risk HPV DNA and TCT,the specificity of TCT detection of cervical lesions was higher than high-risk HPV DNA and HPV DNA+ TCT,the difference was significant (P<0.05). Conclusion The sensitivity and negative predictive value of high-risk HPV DNA testing combined with cytology testing in the cervical cancer screening are higher,and has positive im-plications for screening for cervical lesions.%目的:探讨高危型人乳头状瘤病毒(HPV)DNA检测联合宫颈细胞学检查对宫颈癌前病变筛查的临床意义。方法5080名女性均在2013年2月~2014年1月选择在本院就诊并进行宫颈癌前病变筛查,对其进行宫颈细胞学检查、高危型HPV DNA检测、阴道镜下行宫颈活检,使用统计学方法分析检测结果。结果经组织病理检查确诊ASC-US(不能明确意义的非典型鳞状上皮细胞)组318例,LSIL(低度鳞状上皮内病变)组26例,HSIL(高度鳞状上皮内病变)组52例,浸润癌组6例,HPV DNA+液基薄层细胞检测(TCT)检测宫颈病变的灵敏度及阴性预测值高于高危型HPV DNA检测及TCT,TCT

  15. miRNAs in precancerous lesions of the gastrointestinal tract

    Institute of Scientific and Technical Information of China (English)

    Matteo Fassan; Carlo M Croce; Massimo Rugge

    2011-01-01

    In spite of the well-established understanding of the phenotypic lesions occurring in the shift from native epithelia to invasive (adeno) carcinoma, the molecular typing of the precancerous changes in the gastrointestinal tract remains unreliable. In recent years, no biomarkers have aroused as much interest as the miRNAs, a class of non-coding RNA molecules that function as endogenous silencers of numerous target genes. Aberrant miRNA expression is a hallmark of human disease, including cancer. Unlike most mRNAs, miRNAs are both long-living in vivo and very stable in vitro. Such characteristics allow their testing in paraffin-embedded tissue samples, which is essential in the biological profiling of small (phenotypically characterized) preneoplastic lesions of the gastrointestinal tract (as well as in other fields of human pathology). The upcoming challenge lies in the reliable identification of disease-specific targets of dysregulated miRNAs, to enable miRNA testing in the clinical management of the secondary prevention of gastrointestinal cancer.

  16. Diagnostic validity of cervical precancerous lesions by liquid-based cytology technique combined with high-risk HPV-DNA detection%液基细胞学技术联合高危HPV-DNA检测对宫颈癌前病变的诊断效度

    Institute of Scientific and Technical Information of China (English)

    王泽曼; 陈玉; 郑建鹏

    2011-01-01

    目的:探讨液基细胞学检查技术(LCT)联合高危人乳头状瘤病毒DNA(HPV-DNA)检测诊断宫颈癌前病变的效度.方法:对2007年12月~2010年12月来我院行体检的19~65岁的6 521名女性采用LCT进行宫颈癌的筛查,以及HPV分型基因芯片检测系统进行18种高危HPV基因亚型检测.对上述检测阳性者行阴道镜下宫颈活组织检查,对检测均阴性者依其意愿进行阴道镜下宫颈活组织检查.结果:LCT阳性(≥ASCUS)152例,HPV阳性86例,其中二者均为阳性的有42例,LCT和HPV均为阴性的有6 325例;LCT阳性的152例和HPV阳性的86例中病理活组织检查结果为阳性(≥CIN I)的分别有112例和68例,其中LCT和HPV均阳性的42例中病理活组织检查阳性的有34例.LCT和HPV均为阴性的6 325例中有2 000人自愿行病理检查,其中1人病理检查结果为阳性.LCT诊断宫颈癌前病变的灵敏度为76.19%,特异度为98.05%;HPV检测诊断宫颈癌前病变的灵敏度为46.26%,特异度为99.12%;两方法联合诊断(其中1项阳性即判定为患者)宫颈癌前病变的灵敏度为99.32%,特异度为99.61%.结论:液基细胞检测技术和高危HPV-DNA检测的联合应用优于单项技术检测,对于宫颈癌前病变的筛检具有重要意义.%AIM: To investigate liquid - based cytology technique ( LCT ) combined with high - risk human papilloma virus DNA ( HPV - DNA ) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV - DNA detection in 6 521 women at the age of 19 ~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high - risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results

  17. Microsatellite instability in gastric cancer and pre-cancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Ping Liu; Xiao-Yong Zhang; Yun Shao; Dao-Fu Zhang

    2005-01-01

    AIM: To investigate the microsatellite instability (MSI) in cancer and pre-cancerous lesions of the stomach and its mechanisms underlying the development of gastric cancer.METHODS: Thirty-six gastric cancer samples were obtained from patients undergoing surgery. Forty-one gastric mucosa samples with dysplasia and 51 with intestinal metaplasia (IM) were obtained from patients with chronic gastritis undergoing gastro-endoscopy. Genomic DNA was extracted from the samples. Silver staining single strand conformation polymorphis-polymerize chain reaction (SSCP-PCR) was used to screen MSI markers at 5 loci (Bat-25, Bat-26, D5S346, D17S250, and D2S123)in fresh tissues and formalin-fixed, paraffin-embedded samples and their corresponding normal gastric mucosa.RESULTS: The abnormal shifting of the single-strand DNA (MSI) was identified in 21 out of 36 (58.3%) gastric cancers.Seven cases showed high-level MSI (two or more loci altered) and 14 showed low-level MSI (one locus altered).Gastric cancer with MSI had a tendency to be located in the distal stomach. MSI was also detected in 11 out of 41(26.8%) dysplasia samples and in 9 of 51 (17.6%) IM samples respectively. Three cases of dysplasia and one case of IM showed high-level MSI. Eight cases of dysplasia and 8 cases of IM displayed low-level MSI. MIS in IM was found only in moderate or severe-grade IM. No association was detected between MSI and dysplasia grade.CONCLUSION: Accumulation of MSI in dysplasia and intestinal metaplasia of gastric mucosa may be an early molecular event during gastric carcinogenesis and may contribute to the acquisition of transformed cell phenotype and the development of gastric cancer.

  18. Automated cervical precancerous cells screening system based on Fourier transform infrared spectroscopy features

    Science.gov (United States)

    Jusman, Yessi; Mat Isa, Nor Ashidi; Ng, Siew-Cheok; Hasikin, Khairunnisa; Abu Osman, Noor Azuan

    2016-07-01

    Fourier transform infrared (FTIR) spectroscopy technique can detect the abnormality of a cervical cell that occurs before the morphological change could be observed under the light microscope as employed in conventional techniques. This paper presents developed features extraction for an automated screening system for cervical precancerous cell based on the FTIR spectroscopy as a second opinion to pathologists. The automated system generally consists of the developed features extraction and classification stages. Signal processing techniques are used in the features extraction stage. Then, discriminant analysis and principal component analysis are employed to select dominant features for the classification process. The datasets of the cervical precancerous cells obtained from the feature selection process are classified using a hybrid multilayered perceptron network. The proposed system achieved 92% accuracy.

  19. [Analysis of the reasons for abandoning the follow-up and treatment process in women with pre-cancerous cervical lesions in the province of Jujuy: implications for health management].

    Science.gov (United States)

    Paolino, Melisa; Arrossi, Silvina

    2012-12-01

    Adequate follow-up and treatment of women with high-grade squamous intraepithelial lesion (HSIL) and cancer is key in reducing cervical cancer mortality. This research study aims to analyze the magnitude of abandonment in the follow-up and treatment process, as well as the reasons for this abandonment, in women with HSIL who received care in public health services in the province of Jujuy in 2010. Secondary sources were analyzed and women were interviewed in their homes. The abandonment rate within the study population was 23.5%. Among these women, 40.5% mentioned organizational problems in the health care system as a reason for abandoning and 30.9% mentioned subjective reasons. Our results have been used in the reorganization of the follow-up and treatment process being carried out in the province. PMID:23681458

  20. Research on the Clinical Value of Vaginal Ultrasound in the Diagnosis of Early Cervical Cancer and Precancerous Lesions%阴道超声在早期宫颈癌及癌前病变诊断中的临床价值研究

    Institute of Scientific and Technical Information of China (English)

    张利; 赵华巍; 郭丽娟

    2016-01-01

    目的:探讨阴道超声对早期宫颈癌及癌前病变的诊断价值。方法以43例宫颈癌患者作为研究对象,其中早期宫颈癌27例,癌前病变16例。所有患者均于术前确诊并行手术治疗。另选取体检无妇科病史的30例妇女作为对照组,对三组的阴道超声声像特征进行对比分析。结果阴道超声诊断为宫颈癌前病变者16例,准确率为81.3%;超声诊断为早期宫颈癌者25例,诊断准确率为92.6%。三组的宫颈厚径与宫颈内膜厚度比较存在显著性差异( P<0.05),三组间关系为:正常对照组<癌前病变组<早期宫颈癌组。正常对照组中均未见宫颈线中断与宫颈内出现强回声或低回声团,15例测到丰富血流;癌前病变组宫颈线中断2例,3例可见宫颈内出现强回声或低回声团,血流丰富8例;早期宫颈癌组中宫颈线中断10例,宫颈内出现强回声或低回声团8例,血流丰富22例;三组间存在显著性差异( P<0.05)。3组PSV与RI比较存在显著性差异(P<0.05);PSV:正常对照组<病前病变组<早期宫颈癌组;RI:正常对照组>病前病变组>早期宫颈癌组。结论阴道超声对早期宫颈癌及癌前病变具有较高的敏感度,可为临床提供有价值的信息,可作为细胞学检查与妇科检查的有效补充,减少漏诊或误诊病例的发生。%Objective To study the clinical value of vagianl ultrasound in the diagnosis of early cervical cancer and pre -cancerous lesions .Methods 43 cases of patients with cervical cancer were selected ,in which,27 cases were early cervical cancer and 16 cases were precancerous lesions .All patients were diagnosed before operation .30 cases of women without history of gynae-cology and symptoms were selected as the control group .The characteristics of vaginal ultrasound acoustic images were compared and analyzed.Results Among these cases,16 cases were

  1. 高危型HPV检查和TCT在宫颈癌及癌前病变的早期诊断临床研究%Clinical studies of high-risk HPV examination and TCT in the early diagnosis of cervical cancer and precancerous lesions.

    Institute of Scientific and Technical Information of China (English)

    董静; 倪爱军

    2013-01-01

    Objective To investigate the clinical value of the high - risk types of human papillomavims ( HPV ) examination and thinprep cytologic test ( TCT ) in the early diagnosis of cervical cancer and precancerous lesions. Methods High - risk HPV and TCT examination results in 1248 women screened for cervical cancer were retrospectively analyzed. The clinical diagnostic value of high - risk HPV and TCT examination and the combination of the two methods was compared. Results High - risk HPV infection rate and TCT - positive rate in cervical intraepithelial neo-plasia ( CIN )Ⅰ group, CIN Ⅱ group, CIN Ⅲ group, and cervical cancer group were significantly higher than those in normal and inflammatory group (all P <0. 05 ). The sensitivity, correct diagnosis index, negative predictive value and positive predictive value of the combined detection improved to varying degrees and they were 92. 2% , 0.71, 99. 6% and 40. 2% , respectively. Conclusion The combined detection of high - risk HPV and TCT has important clinical significance for the early diagnosis of cervical cancer.%目的 研究高危型人乳头瘤病毒(HPV)检查及薄层液基细胞学技术(TCT)在宫颈癌与癌前病变的早期诊断的临床应用价值.方法 回顾性分析高危型HPV检查及TCT对1 248例宫颈癌筛查对象的检查结果.比较两种方法及联合应用的临床诊断价值.结果 病理组织学CINⅠ期组、CINⅡ期组、CINⅢ期组、宫颈癌组高危型HPV感染率及TCT阳性率与正常及炎症组比较均有统计学差异(P<0.05);CINⅠ-Ⅲ期及宫颈癌组高危型HPV感染率、TCT阳性率均高于正常及炎症组;高危型HPV检测与TCT两种方法联合检测灵敏度(92.2%),正确诊断指数(0.71)、阴性预测值(99.6%)及阳性预测值(40.2%)均有不同程度的提高.结论 高危型HPV检测与TCT联合对宫颈癌早期发现、早期诊断有重要的临床意义.

  2. Effect of Helicobacter pylori infection on Bax protein expression in patients with gastric precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Hai-Feng Liu; Wei-Wen Liu; Guo-An Wang; Xiao-Chun Teng

    2005-01-01

    AIM: To investigate the effect of Helicobacter pylori (H pylori) infection on Bax protein expression, and explore the role of H pylori in gastric carcinogenesis.METHODS: H pylori was assessed by rapid urease test and Warthin-Starry method, and expression of Bax protein was examined immunohistochemically in 72 patients with pre-malignant lesions.RESULTS: Bax protein was differently expressed in intestinal metaplasia and gastric dysplasia, and showed 63.99% positivity. The positivity of Bax protein expression in H pylori-positive gastric precancerous lesions (72.3%) was significantly higher than that in H pylori-negative gastric precancerous lesions (48.0%, χ2 = 4.191, P<0.05).H pylori infection was well correlated with the expression of Bax protein in gastric precancerous lesions (r = 0.978,P<0.01). After eradication of H pylori, the positivity of Bax protein expression significantly decreased in H pylori-positive gastric precancerous lesions (χ2= 5.506,P<0.05). In the persisting H pylori-infected patients,the positivity of Bax protein expression was not changed.CONCLUSION: H pylori infection may be involved in the upregulation of Bax gene, which might be one of the mechanisms of H pylori infection-induced gastric epithelial cell apoptosis. H pylori might act as a tumor promoter in the genesis of gastric carcinoma and eradication of H pylori could inhibit gastric carcinogenesis.

  3. Clinical significance of fluorescence in situ hybridization for detection of hTERC gene amplification in cervical cancer and precancerous tissues cases

    Directory of Open Access Journals (Sweden)

    Shuang LIU

    2012-06-01

    Full Text Available Objective  To detect the human telomerase RNA gene (hTERC amplification in cervical lesions, and explore its clinical significance. Methods  The tissues of the cervical lesions were collected from 195 patients, including 33 of chronic cervicitis, 34 of CINⅠ, 37 of CIN Ⅱ-Ⅲ, 30 of cervical squamous cell carcinoma, and 61 of cervica1 adenocarcinoma, and abnormal hTERC was detected with amplification of fluorescence in situhybridization (FISH. The relationship between hTERC gene amplification and clinicopathological parameters was analyzed. Results  Among the 195 patients, the positive rate of hTERC gene amplification was 3.03% (1/33, 29.41% (10/34, 72.97% (27/37, 100% (30/30, 91.8% (56/61 in chronic cervicitis, CINⅠ, CIN Ⅱ-Ⅲ, cervical squamous cell carcinoma and cervica1 adenocarcinoma respectively, and the results showed that hTERC amplification rate was significantly higher in group CIN Ⅱ-Ⅲthan in group CINⅠ(P 0.05. Conclusion  Detection of gene amplification by FISH technology can be used as a means for accurate diagnosis and prediction of the histologically difficult-to-diagnose lesion and for risk assessment after treatment of cervical precancerous lesions.

  4. Anti- Helicobacter pylori therapy followed by celecoxib on progression of gastric precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Li-Jing Zhang; Shi-Yan Wang; Xiao-Hui Huo; Zhen-Long Zhu; Jian-Kun Chu; Jin-Cheng Ma; Dong-Sheng Cui; Ping Gu; Zeng-Ren Zhao; Ming-Wei Wang; Jun Yu

    2009-01-01

    AIM:To evaluate whether celecoxib,a selective cyclooxygenase 2 (COX-2) inhibitor,could reduce the severity of gastric precancerous lesions following Helicobacter pylori (H pylori) eradication.METHODS:H pylori-eradicated patients with gastric precancerous lesions randomly received either celecoxib (n=30) or placebo (n=30) for up to 3 mo.COX-2 expression and activity was determined by immunostaining and prostaglandin E2 (PGE2) assay,cell proliferation by Ki-67 immunostaining,apoptosis by TUNEL staining and angiogenesis by microvascular density (MVD) assay using CD31 staining.RESULTS:COX-2 protein expression was significantly increased in gastric precancerous lesions (atrophy,intestinal metaplasia and dysplasia,respectively) compared with chronic gastritis,and was concomitant with an increase in cell proliferation and angiogenesis.A significant improvement in precancerous lesions was observed in patients who received celecoxib compared with those who received placebo (P<0.001).Of these three changes,84.6% of sites with dysplasia regressed in patients treated with celecoxib (P=0.002) compared with 60% in the placebo group,suggesting that celecoxib was effective on the regression of dysplasia.COX-2 protein expression (P<0.001) and COX-2 activity (P<0.001) in the gastric tissues were consistently lower in celecoxib-treated patients compared with the placebo-treated subjects.Moreover,it was also shown that celecoxib suppressed cell proliferation (P<0.01),induced cell apoptosis (P<0.01) and inhibited angiogenesis with decreased MVD (P<0.001).However,all of these effects were not seen in placebo-treated subjects.Furthermore,COX-2 inhibition resulted in the up-regulation of PPARg expression,a protective molecule with anti-neoplastic effects.CONCLUSION:H pylori eradication therapy followed by celecoxib treatment improves gastric precancerous lesions by inhibiting COX-2 activity,inducing apoptosis,and suppressing cell proliferation and angiogenesis.

  5. The study of thinprep cytology test combined truscreen in early diagnosis cervical precancerous lesions%膜式薄层液基细胞学检查联合初善仪在子宫颈癌前病变诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    张小鹏; 罗新; 王晓玉

    2012-01-01

    目的 探讨膜式薄层液基细胞学检查(thinprep cytologic test,TCT)联合初善仪(truscreen,TS)在子宫颈癌前病变诊断中的应用价值.方法 对30例阴道分泌物异常的患者同时行TCT和TS检查,再行阴道镜检查及组织活检,以组织病理学检查结果作为诊断的金标准.比较2种检查方法单独及联合使用与组织病理学检查结果的吻合程度.结果 30例患者中有25例病理学诊断为子宫颈上皮内瘤变1级及以上病变,为真阳性.TCT测试细胞学意义不明的不典型鳞状上皮细胞以上的阳性19例(63.3%),假阴性6例,漏诊率20.0%,真阳性16例,敏感度84.2%.TS测试阳性18例(60.0%),假阴性7例,漏诊率23.3%,真阳性14例,敏感度77.8%.TCT联合TS测试阳性27例(90.0%),假阴性1例,漏诊率3.3%,真阳性24例,敏感度88.9%.TCT联合TS检测子宫颈癌前病变的阳性率与病理学检查的阳性率分别为90.0%和83.3%,差异无统计学意义(P>0.05).结论 TCT联合TS检查诊断子宫颈癌前病变的准确性明显优于任一单项检查,其漏诊率显著下降.%Objective: To investigate the application value of thinprep cytology test(TCT) combined truscreen(TS) in early diagnosis cervical precancerous lesions. Methods:Thirty patients with abnormal vaginal secretions were detected under TCT and TS,and then vaginal tissue biopsies were tested under colposcopy. The results of pathological examination acted as the gold standard of diagnosis. The match degrees of single and combined using methods and histopathological examination results were compared. Results: The results of histopathological examination of 25 cases were cervical intraepithelial neoplasia grade 1 or above. Nineteen cases(63. 3% ) were ASCUS cytology positive,6 cases were false negative,16 cases were true postive,the rate of fail to diagnosis was 20.0% ,and the sensitiveness degree was 84. 2% under TCT. Compared with TCT results,the data were 18 cases(60. 0% ) ,7 cases, 14 cases,23. 3% and

  6. Oral precancerous lesions: Problems of early detection and oral cancer prevention

    Science.gov (United States)

    Gileva, Olga S.; Libik, Tatiana V.; Danilov, Konstantin V.

    2016-08-01

    The study presents the results of the research in the structure, local and systemic risk factors, peculiarities of the clinical manifestation, and quality of primary diagnosis of precancerous oral mucosa lesions (OMLs). In the study a wide range of OMLs and high (25.4%) proportion of oral precancerous lesions (OPLs) in their structure was indicated. The high percentage of different diagnostic errors and the lack of oncological awareness of dental practitioners, as well as the sharp necessity of inclusion of precancer/cancer early detection techniques into their daily practice were noted. The effectiveness of chemilumenescence system of early OPLs and oral cancer detection was demonstrated, the prospects of infrared thermography as a diagnostic tool were also discussed.

  7. Study on Translational Medicine of RUNX3 Expression in Oral Precancerous Lesions and Squamous Cell Carcinoma

    Institute of Scientific and Technical Information of China (English)

    Ramamurthy Mustafa; Shabil Mohamed Rajaraman

    2016-01-01

    Objective: To explore the expression and distribution characteristics of RUNX3 in the pathological process of oral squamous cell carcinoma (OSCC) as well as its correlation with clinicopathological features. Methods: Fifteen cases of normal oral mucosal tissues, 37 cases of OSCC tissues and 21 cases of precancerous lesion tissues were respectively collected. The expression and distribution characteristics of RUNX3 were detected using immunohistochemical method, and its relationship with each clinicopathological feature of OSCC was analyzed. Results: The positive rates of RUNX3 expression in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues were 100% (15/15), 95.24% (20/21) and 54.05% (20/37), and their average optical densities were (0.391±0.07), (0.369±0.03) and (0.263±0.01), respectively. The differences were statistically signiifcant by comparison to the positive rates of RUNX3 expression and their average optical densities in normal oral mucosal tissue, precancerous lesion tissue and OSCC tissue (P0.05), but the difference was statistically signiifcant among the patients with different pathological gradings (P=0.012). Conclusion: The expression of RUNX3 in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues gradually shows a down-regulating tendency and its frequency of abnormal localization is on the rise. The expression of RUNX3 is closely associated with the degree of histological differentiation. All of these findings indicate that RUNX3, an important action factor in the occurrence and progression of OSCC, can be considered as a key biological indicator for early diagnosis, judgment of malignant degree and prognostic monitoring.

  8. Study on Translational Medicine of RUNX3 Expression in Oral Precancerous Lesions and Squamous Cell Carcinoma

    Directory of Open Access Journals (Sweden)

    Ramamurthy Mustafa

    2016-03-01

    Full Text Available Objective: To explore the expression and distribution characteristics of RUNX3 in the pathological process of oralsquamous cell carcinoma (OSCC as well as its correlation with clinicopathological features. Methods: Fifteen cases of normal oral mucosal tissues, 37 cases of OSCC tissues and 21 cases of precancerous lesion tissues were respectively collected. The expression and distribution characteristics of RUNX3 were detected using immunohistochemical method, and its relationship with each clinicopathological feature of OSCC was analyzed. Results: The positive rates of RUNX3 expression in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues were 100% (15/15, 95.24% (20/21 and 54.05% (20/37, and their average optical densities were (0.391±0.07, (0.369±0.03 and (0.263±0.01, respectively. The differences were statistically significant by comparison to the positive rates of RUNX3 expression and their average optical densities in normal oral mucosal tissue, precancerous lesion tissue and OSCC tissue (P<0.01. There was no statistical significance regarding RUNX3 expression in the patients with different gender, age and presence or absence of lymph node metastasis (P>0.05, but the difference was statistically significant among the patients with different pathological gradings (P=0.012. Conclusion: The expression of RUNX3 in normal oral mucosal tissues, precancerous lesion tissues and OSCC tissues gradually shows a down-regulating tendency and its frequency of abnormal localization is on the rise. The expression of RUNX3 is closely associated with the degree of histological differentiation. All of these findings indicate that RUNX3, an important action factor in the occurrence and progression of OSCC, can be considered as a key biological indicator for early diagnosis, judgment of malignant degree and prognostic monitoring.

  9. Awareness regarding oral cancer and oral precancerous lesions among rural population of Belgaum district, India.

    Science.gov (United States)

    Sankeshwari, Roopali; Ankola, Anil; Hebbal, Mamata; Muttagi, Sidramesh; Rawal, Nilam

    2016-09-01

    Belgaum district of Karnataka state is well known for high production and consumption of tobacco in Southern India. This study aimed to investigate the rural population's awareness of oral cancer, precancerous lesions and their risk factors. Data were collected via face to face interviews using a pretested and validated questionnaire. The questionnaire comprised two parts: part one had questions concerning socio-demographic data and part two consisted of 25 questions pertaining to people's attitudes to and awareness of risk factors for oral cancer and precancerous lesions. One researcher interviewed participants and recorded the responses verbatim. Of the participants, 17% identified all the symptoms of oral cancer and 27.8% identified all the symptoms of oral precancerous lesions. Approximately 90% of the participants had never noticed statutory warnings on tobacco and alcohol products. Awareness was especially poor in people of lower socio-economic status. This study highlights a need for education concerning the risk factors for oral cancer, its clinical manifestations and the impact of adverse habits on long term health. Health education campaigns emphasizing oral cancer need to be integrated with broader public health messages. PMID:25758170

  10. Glycoproteomic analysis of serum from patients with gastric precancerous lesions

    DEFF Research Database (Denmark)

    Gomes, Catarina; Almeida, Andreia; Ferreira, José Alexandre;

    2013-01-01

    Gastric cancer is preceded by a carcinogenesis pathway that includes gastritis caused by Helicobacter pylori infection, chronic atrophic gastritis that may progress to intestinal metaplasia (IM), dysplasia, and ultimately gastric carcinoma of the more common intestinal subtype. The identification...... of glycosylation changes in circulating serum proteins in patients with precursor lesions of gastric cancer is of high interest and represents a source of putative new biomarkers for early diagnosis and intervention. This study applies a glycoproteomic approach to identify altered glycoproteins expressing...... the simple mucin-type carbohydrate antigens T and STn in the serum of patients with gastritis, IM (complete and incomplete subtypes), and control healthy individuals. The immunohistochemistry analysis of the gastric mucosa of these patients showed expression of T and STn antigens in gastric lesions, with STn...

  11. Narrow-band imaging in the diagnosis of early esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    HUANG Liu-ye; CUI Jun; WU Cheng-rong; LIU Yun-xiang; XU Ning

    2009-01-01

    Background In the recent years,the incidence of esophageal cancer in China has increased.The key point for raising the survival rate is the diagnosis and treatment at an early stage.Narrow-band imaging (NBI) can enhance the contrast of the mucous membrane of the esophagus without staining.This study aimed to explore the value of NBI in the diagnosis of early esophageal cancer and precancerous lesions.Methods The esophagus was examined with ordinary endoscopy and NBI endoscopy.Pit patterns and blood capillary forms were examined with routine magnifying endoscopy and NBI endoscopy.Finally,a 1.2% Lugoul's iodine solution was used to stain the esophageal mucosal surface and a biopsy was taken at all the sites where NBI or iodine staining was positive.NBI and iodine staining scales were compared with pathologic diagnosis,which was considered as the gold standard.Results A total of 90 cases (138 lesions in total) were diagnosed as early esophageal cancer or precancerous lesions:104 lesions (75.4%) were detected with ordinary endoscopy,120 lesions (87.0%) were detected with NBI endoscopy,and 138 lesions (100%) were detected with iodine staining.The lesion detection rate of NBI was significantly lower than that of iodine staining (X2=17.176,P <0.01).However,there was no significant difference between NBI and iodine staining for the diagnosis of high grade intraepithelial neoplasia (X2=1.362,P >0.05),while the detection rate of NBI was significantly lower than that of iodine staining for the diagnosis of low grade intraepithelial neoplasia (X2=13.388,P <0.01).The pit pattern and blood capillary form of eady esophageal cancer and precancerous lesions could be demonstrated clearer with NBI than with ordinary endoscopy.Conclusions NBI can enhance the contrast of the mucous membrane of the esophagus without staining.The combination of NBI and iodine staining can raise the diagnostic rate of early esophageal cancer and precancerous lesions.

  12. Cervical syphilitic lesions mimicking cervical cancer: a rare case report

    Directory of Open Access Journals (Sweden)

    Xiaoqing Zhu

    2015-02-01

    Full Text Available A woman presented to the hospital due to postcoital vaginal bleeding. The patient was initially diagnosed with cervical carcinoma by clinicians at a local hospital. However, a biopsy of the cervical lesions revealed chronic inflammation and erosion of the cervical mucosa, and the rapid plasma reagin ratio titer was 1:256. The patient was eventually diagnosed with syphilitic cervicitis and treated with minocycline 0.1 g twice a day. The patient was cured with this treatment.

  13. 血浆sHLA-G在宫颈癌和癌前病变检测中的应用%The application of plasma soluble human leukocyte antigen in diagnosis of patients with cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    张欣; 郑妮; 王传新; 朱成宝; 王丽丽; 杜鲁涛; 王顺

    2011-01-01

    康对照组(U值分别为7.564、4.522、7.429,P均<0.05);CIN Ⅲ级组血浆CA125水平明显高于CIN Ⅰ级组和健康对照组(U值分别为5.871、5.435,P均<0.05).ROC曲线分析sHLA-G的AUC为0.828(95% CI:0.768~0.879),其诊断价值高于SCC-Ag[0.727(95% CI:0.658~0.788);Z=2.294,P<0.05]和CA125[0.705(95%CI:0.636~0.769);Z=2.842,P<0.05];SCC-Ag与CA125的诊断效能比较,差异无统计学意义(Z=0.672,P>0.05).经ROC曲线分析确定sHLA-G诊断临界值为109.6 kU/L时,其敏感度为86.3%,特异度为76.1%,阳性预测值为80.0%,阴性预测值为83.3%,准确性为78.4%.宫颈癌患者血浆sHLA-G水平与FIGO分期及淋巴结转移密切相关(U值分别为6.085、4.451,P均<0.05),而与患者年龄、肿瘤大小、组织形态及细胞分化无关(U值分别为1.274、1.956、1.268、2.719,P均>0.05).结论 血浆sHLA-G水平检测有助于宫颈癌及癌前病变的早期发现,并且可以作为宫颈癌病情进展及淋巴结转移的判断指标.%Objective To explore the application value of plasma sHLA-G in diagnosis of CIN and cervical cancer. Methods The plasma sHLA-G levels were detected by ELISA in 102 cases with cervical cancer( FIGO Ⅰ stage 32 cases, Ⅱ stage 28 cases, Ⅲ stage 25 cases and Ⅳstage 17 cases; tumor size:<4 cm 63 cases and ≥4 cm 39 cases; squamous cell carcinoma 78 cases and adenocarcinoma 24 cases;cell differentiation:well 57 cases, moderate 29 cases and poor 16 cases; lymph nodes metastasis negative64 cases and positive 38 cases ), 72 cases with CIN( Ⅰ grade 21 cases, Ⅱ grade 25 cases and Ⅲ grade26 cases ) and 20 cases of healthy controls. The diagnostic value of sHLA-G and its correlations with clinical parameters were analyzed. Results The plasma levels of sHLA-G were 193.6( 151.3-287.4 ) kU/L in cervical cancer group, 48.3( 34.6-57.2 ) kU/L in CIN Ⅰ group, 91.3( 68.2-118.6 ) kU/L in CIN Ⅱ group, 106.4( 73.8-165.7 ) kU/L in CIN Ⅲ group and 45.2( 38.0-55.5 ) kU/L in health control group.The level of s

  14. Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Ming-Yao Wu; Ying-Rui Liang; Xian-Ying Wu; Chu-Xiang Zhuang

    2002-01-01

    AIM: To study the expression of early growth response gene1 (Egr-1 gene) and Bcl-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC) and precancerous lesions.METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa.RESULTS: Egr-1 gene in situ hybridization, Bcl-X/L immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01).The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC.CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.

  15. Clinical significance of detection of human telomerase reverse transcriptase in colorectal cancer and its precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    刘少平

    2014-01-01

    Objective To evaluate the expression and clinical significance of human telomerase reverse transcriptase(h TERT)in colorectal cancer and its precancerous lesion.Methods The levels of h TERT expression were detected by immunohistochemistry in colorectal cancers(n=95),colorectal adenomatous polyposis(n=30)and normal colorectal tissues(n=30).The relationship between the expression of h TERT in colorectal cancer tissues and the pathologic features and prognosis were analyzed.Results The positive rate of h TERT expression

  16. Characterization of breast precancerous lesions and myoepithelial hyperplasia in sclerosing adenosis with apocrine metaplasia

    DEFF Research Database (Denmark)

    Celis, J.E.; Gromova, I.; Cabezón, T.;

    2007-01-01

    . First, we identify specific protein biomarkers for benign apocrine metaplasia and thereafter we search for biomarkers that are highly overexpressed by pure invasive apocrine carcinomas. Here we present studies in which we have used antibodies against components of a benign apocrine signature...... the three markers associated with pure invasive apocrine carcinomas. These studies also revealed p53 positive, non-apocrine putative precancerous lesions as well as novel phenotypes for ME and some luminal cells characterized by the expression of cytokeratin 15. © 2007 Federation of European Biochemical...

  17. A preliminary study on ras protein expression in human esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Jian Li; Chang Wei Feng; Zhi Guo Zhao; Qi Zhou; Li Dong Wang

    2000-01-01

    @@INTRODUCTION The esophageal carcinoma is a common malignant tumor in Linzhou City (Linxian) of Henan Province in northern China. Although the etiology and natural history of esophageal carcinoma are not clear, a substantial amount of evidence has been provided to suggest that the development of human esophageal squamous cell carcinomas (SCC) is a multistage progressive process[1-4] An early indicator of abnormality in persons predisposed to esophageal SCC is an increased proliferation of esophageal epithelial cells,morphologically manifested as basal cell hyperplasia (BCH), and dysplasia (DYS), and carcinoma in situ, which could be considered precancerous lesions of esophageal SCC[1-4].

  18. A STUDY ON THE ROLE OF MICRO NUCLEI IN ASSESSING THE PROGRESSION OF PRECANCEROUS LESIONS OF CERVIX AND THE DIAGNOSIS OF CARCINOMA OF CERVIX

    Directory of Open Access Journals (Sweden)

    Anitha

    2015-06-01

    Full Text Available BACKGROUND: Invasive squamous cell carcinoma of cervix remains the most common malignant neoplasm of the female genital tract in many countries. The Papanicolaou stained cervical smear is an excellent and effective method in the diagnosis of invasive carcinoma and precancerous lesions of the cervix. This study was conducted to assess the value of Feulgen stained cervical smears in elucidating nuclear features help ful in the diagnosis of malignancy over conventional Pap stained smears and also to look for micronucleated cells in suspected cases of carcinoma of cervix. AIM: To analyse the distribution of cases of invasive squamous cell carcinoma and intraepithelial n eoplasia (squamous intraepithelial lesion of cervix over a period of 3 years, to elucidate additional nuclear features helpful in diagnosis of carcinoma using Feulgen stained cervical smears and to study the distribution of micronuclei in Feulgen stained smears from suspected cases of Carcinoma cervix. MATERIALS AND METHODS: A three year analysis of all cases of dysplasia and invasive carcinoma of cervix was done by reviewing Papanicolaou stained cervical smears from all the diagnosed cases of invasive car cinoma and precancerous lesions of the cervix. Cervical smears from sixty clinically suspected cases of carcinoma of cervix and smears from 10 normal women collected during a period of 12 months were studied in detail using Papanicolaou stained and Feulgen Stained Smears and micronuclei analysis (MN analysis was done. RESULTS : A total of 24343 cervical smears were screened during the 3 year period of study. Out of these 24343 cases there were 267(1.09% cases of cervical intraepithelial neoplasia and 144(0 .592% cases of invasive squamous cell carcinoma. Micronuclei analysis done using Feulgen Stained Smears demonstrated a consistent increase in micronucleated cells proportional to the increase in severity of th e lesion from normal to invasive carcinoma. CO NCLUSION : This study

  19. Secondary prevention of cervical cancer through the development and implementation of a system to optimize diagnostic and therapeutic and rehabilitation measures in the background and precancerous cervical diseases

    Directory of Open Access Journals (Sweden)

    F. F. Badretdinova

    2012-01-01

    Full Text Available The results of a comprehensive evaluation and treatment of background and pre-cancerous cervical cancer of women were studied (n = 1022. There is the complex assessment of social and obstetric gynecological risk factors for cervical intraepithelial neoplasia and cervical cancer. A system for optimizing diagnostic, therapeutic, preventive and rehabilitative measures, taking into account the differentiated approach to the choice of treatment, follow-up in the near and long-term postoperative period. An individual approach to the selection of organ presentation or radical treatment using new technologies of surgical treatment are identified. Application of the developed system enabled a statistically significantly improve the results of treatment of background and precancerous cervical disease.

  20. THE ROLE OF HELICOBACTER PYLORI IN GASTRIC CANCER AND PRECANCEROUS LESIONS

    Institute of Scientific and Technical Information of China (English)

    任刚; 蔡嵘; 陈强; 许幼如; 张文竹; 奚政君

    2002-01-01

    Objective To determine the role of Helicobacter pylori ( Hp ) in the development of gastric cancer and gastric precancerous lesions. Methods From the biopsy specintestinal metaplasiaens of gastric cancer and precancerous lesions Hp organisms were detected by a combination use of three methods ( Clo-test,Giemsa and PCR ). Mutations of C-Ha-ras and p53 genes were determined by polymerase chain reaction-single strand conformation polymorphism analysis-direct sequencing ( PCR-SSCP-S ). Results The detection rates of Hp in gastric cancer, dysplasia and intestinal metaplasia were significantly higher than that of normal gastric mucosa, and the mutational rates of C-Ha-ras and p53 genes in Hp positive patients were significantly higher than those in Hp negative patients. In gastric cancer patients, the mutational rate of p53 gene in Hp positive patients was significantly higher than that of Hp negative patients, in which spot mutation was the main pattern of gene changes. Conclusion Hp infection might be related to gastrocarcinogenesis and gene mutation might play a role in Hp related gastric cancer.

  1. Optical detection of cancer and precancerous lesions of the upper aerodigestive tract: methods for assessment of vertical extensions

    Science.gov (United States)

    Kraft, Marcel; Arens, Christoph

    2013-06-01

    Introduction: Endoscopic imaging can be used in the assessment of cancer and precancerous lesions of the upper aerodigestive tract. The objective of this presentation is to describe vertical imaging methods. Methods: The working principle and technique of optical coherence tomography (OCT) as well as endosonography (EUS) are briefly illustrated. Results: The main indications, normal and pathologic findings of each method, frequent pitfalls, and clinical results up to now are presented in detail. Conclusions: Endosonography helps in determining the exact extension of advanced carcinomas beyond 3 mm, while OCT, due to its better resolution, is superior in the assessment of precancerous and early cancerous lesions up to 2 mm thickness.

  2. In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix

    Energy Technology Data Exchange (ETDEWEB)

    Mourant, Judith R [Los Alamos National Laboratory

    2008-01-01

    A noninvasive optical diagnostic system for detection of cancerous and precancerous lesions of the cervix was evaluated in vivo. The optical system included a fiber-optic probe designed to measure polarized and unpolarized light transport properties of a small volume of tissue. An algorithm for diagnosing tissue based on the optical measurements was developed that used four optical properties, three of which were related to light scattering properties and the fourth of which was related to hemoglobin concentration. A sensitivity of {approx}77% and specificities in the mid 60% range were obtained for separating high grade squamous intraepithelial lesions and cancer from other pathologies and normal tissue. The use of different cross-validation methods in algorithm development is analyzed, and the relative difficulties of diagnosing certain pathologies are assessed. Furthermore, the robustness of the optical system for use by different doctors and to changes in fiber-optic probe are also assessed, and potential improvements in the optical system are discussed.

  3. Application of combined detection of HR-HPV and TCT in screening for cervical cancer and precancerous lesions%高危型人乳头瘤病毒检测联合薄层液基细胞学检查在宫颈疾病筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    叶波; 宋晓玉

    2015-01-01

    目的:进行高危型人乳头瘤病毒(HR-HPV)检测,分析液基薄层细胞学(TCT)检测与组织病理学诊断结果的符合情况,探讨二者联合检测对宫颈癌及癌前病变的筛查价值和意义。方法对1374例妇瘤科就诊患者,进行 HR-HPV、TCT 联合检测,对结果进行对比分析。结果684例意义不明的不典型鳞状细胞(ASCUS)患者病理学诊断结果中,炎症占51.3%,不典型增生轻度(CINⅠ)占47.4%;375例低度鳞状上皮内病变(LSIL)患者病理学诊断结果中,CINⅠ占48.5%,CINⅡ~Ⅲ占21.3%;294例高度鳞状上皮内病变(HSIL)患者病理学诊断结果中,CINⅠ占24.5%,CINⅡ~Ⅲ占62.9%;20例 SCC 患者病理学诊断结果中,CINⅡ~Ⅲ占20%,宫颈癌占80%。统计学分析显示,TCT 诊断与组织病理学诊断构成比差异有统计学意义(P <0.05)。HR-HPV 阳性率为74.3%,阴性率为25.7%。HR-HPV 阳性率随病理级别的升高而显著升高。HR-HPV 阳性组与阴性组病理诊断级别构成比差异有统计学意义(P <0.05)。TCT 与高危型 HPV 联合检测时,其灵敏度为76.5%,特异度为80.3%,联合检测的灵敏度和 TCT 差异有统计学意义(P <0.05),联合检测的特异度和 HR-HPV 差异有统计学意义(P <0.05)。结论 HR-HPV 检测与 TCT 联合能够提高宫颈病变的检出率,是一种较为理想的宫颈癌筛查方法。%Objective To study the status of coincidence of high-risk human papillomavirus(HR-HPV)test and thinprep cytolo-gy test with biopsy histopathologic diagnosis.And to investigate the value of combined detection in the diagnosis of the cervical cancer and precancerous lesions.Methods High-risk HPV test and thinprep cytology test were taken simultaneously in 1374 pa-tients visiting department of gynecological oncology.The diagnostic value of combination assay of these indices was evaluated.Re-sults In 684 patients with ASCUS pathology diagnosis,inflammation accounted for 5 1.3% and CINⅠ accounted for 47.4%.A-mong the

  4. Assessment of hemodynamics in precancerous lesion of hepatocellular carcinoma: Evaluation with MR perfusion

    Institute of Scientific and Technical Information of China (English)

    Sheng Guan; Wei-Dong Zhao; Kang-Rong Zhou; Wei-Jun Peng; Feng Tang; Jian Mao

    2007-01-01

    AIM: To investigate the hemodynamic changes in a precancerous lesion model of hepatocellular carcinoma (HCC).METHODS: Hemodynamic changes in 18 Wistar rats were studied with non-invasive magnetic resonance (MR)perfusion. The changes induced by diethylnitrosamine (DEN) developed into liver nodular lesions due to hepatic cirrhosis during the progression of carcinogenesis.The MR perfusion data [positive enhancement integral (PEI)] were compared between the nodular lesions corresponding well with MR images and pathology and their surrounding hepatic parenchyma.RESULTS: A total of 46 nodules were located by MR imaging and autopsy, including 22 dysplastic nodules (DN), 9 regenerative nodules (RN), 10 early HCCs and 5 overt HCCs. Among the 22 DNs, 6 were low-grade DN (LGDN) and 16 were high-grade DN (HGDN). The average PEI of RN, DN, early and overt HCC was 205.67± 31.17, 161.94 ± 20.74, 226.09 ± 34.83, 491.86 ±44.61 respectively, and their liver parenchyma nearby was 204.84 ± 70.19. Comparison of the blood perfusion index between each RN and its surrounding hepatic parenchyma showed no statistically significant difference (P = 0.06). There were significant differences in DN (P = 0.02). During the late hepatic arterial phase, the perfusion curve in DN declined. DN had an iso-signal intensity at the early hepatic arterial phase and a low signal intensity at the portal venous phase. Of the 10early HCCs, 4 demonstrated less blood perfusion and 6 displayed minimally increased blood flow compared to the surrounding parenchyma. Five HCCs showed significantly increased blood supply compared to the surrounding parenchyma (P = 0.02).CONCLUSION: Non-invasive MR perfusion can detect changes in blood supply of precancerous lesions.

  5. Dynamic expression of pepsinogen C in gastric cancer, precancerous lesions and Helicobacter pylori associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Pei-Fang Ning; Hui-Jie Liu; Yuan Yuan

    2005-01-01

    AIM: To investigate the relationship between the expression of pepsinogen C (PGC) and gastric cancer, precancerous diseases, and Helicobacter pylori ( H pylori) infection. METHODS: The expression of PGC was determined by immunohistochemistry method in 430 cases of gastric mucosa. H pylori infection was determined by HE staining, PCR and ELISA in 318 specimens.RESULTS: The positive rate of PGC expression in 54 cases of normal gastric mucosa was 100%. The positive rates of PGC expression in superficial gastritis or gastric ulcer or erosion, atrophic gastritis or gastric dysplasia and gastric cancer decreased significantly in sequence (P<0.05;100%/89.2% vs 14.3%/15.2% vs 2.4%). The overexpression rate of PGC in group of superficial gastritis with H pylori infection was higher than that in group without H pylori infection (P<0.05; χ2= 0.032 28/33 vs 15/25).The positive rate of PGC expression in group of atrophic gastritis with H pylori infection was lower than that in group without H pylori infection (P<0.01; χ2 = 0.003 4/61vs 9/30), and in dysplasia and gastric cancer. CONCLUSION: The level of PGC expression has a close relationship with the degree of malignancy of gastric mucosa and development of gastric lesions. There is a relationship between H pylori infection and expression of antigen PGC in gastric mucosa, the positive rate of PGC expression increases in early stage of gastric lesions with H pylori infection such as gastric inflammation and decreases during the late stage such as precancerous diseases and gastric cancer. PGC-negative cases with H pylori-positive gastric lesions should be given special attention.

  6. 血清可溶性白细胞G抗原在宫颈癌及癌前病变的临床诊断意义%Clinical diagnostic value of plasma sHLA-G antigen in cervical cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    田玫玲; 卢庆乐

    2015-01-01

    断指标。但其与发生宫颈癌及癌前病变的特异性分析仍有待进一步的相关研究。%Objective To explore the clinical diagnostic valve of serum soluble human leukocyte antigen‐G (sHLA‐G) in cervical cancer and precancerous lesion cervical intraepithelial neoplasia(CIN) .Methods The serum sHLA‐G level was detected by using ELISA and serum TA‐4 and SCC‐Ag levels were detected by using the light‐emitting electrochemical immunoassay method detec‐ting in 230 cases of cervical carcinoma ,120 cases of CIN and 30 healthy volunteers .The differences among various groups and their relationship with the clinicopathological features of cervical cancer were analyzed .Results (1) The comparison of serum sHLA‐G , TA‐4 and SCC‐Ag levels :there were statistically significant differences in serum sHLA‐G level among various groups (P=0 .000);the serum sHLA‐G level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰgroup , CIN Ⅱ group and CIN Ⅲ group (P=0 .000 ,P=0 .000 ,P=0 .002 ,P=0 .006);which in the CIN Ⅲ group was significantly higher than that in the CIN Ⅰ group and the healthy control group (P=0 .001 ,P=0 .021) .There were statistically significant differences in serum TA‐4 level among various groups (P=0 .006);the serum TA‐4 level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .003 ,P=0 .008 ,P=0 .018);which in the CIN Ⅲgroup was significantly higher than that in the healthy control group and the CIN Ⅰ group (P=0 .023 ,P=0 .031) .The differences of serum SCC‐Ag level among various groups had statistically significant differences (P=0 .000);which in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .000 ,P=0 .001 ,P=0 .007) , and which in the CIN Ⅲ group was significantly higher than that in the healthy

  7. Detection of aberrant methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Zhao-Hui Huang; Li-Hua Li; Fan Yang; Jin-Fu Wang

    2007-01-01

    AIM: To investigate the feasibility of detecting methylated fecal DNA as a screening tool for colorectal carcinoma (CRC) and precancerous lesions.METHODS: Methylated secreted frizzled-related protein gene 2 (SFRP2), hyperplastic polyposis protein gene (HPP1) and O6-methylguanine-DNA methyltransferase gene (MGMT) in stools from 52 patients with CRC, 35 patients with benign colorectal diseases and 24 normal individuals were analyzed using methylation-specific PCR.RESULTS: Methylated SFRP2, HPP1 and MGMT were detected in 94.2%, 71.2%, 48.1% of CRC patients and 52.4%, 57.1%, 28.6% of adenoma patients, respectively. The overall prevalence of fecal DNA with at least one methylated gene was 96.2% and 81.8% in patients with CRC and precancerous lesions, respectively. In contrast, only one of the 24 normal individuals revealed methylated DNA. These results indicated a 93.7% sensitivity and a 77.1% specificity of the assay for detecting CRC and precancerous lesions.CONCLUSION: Methylation testing of fecal DNA using a panel of epigenetic markers may be a simple and promising non-invasive screening method for CRC and precancerous lesions.

  8. Screening for Precancerous Lesions of Upper Gastrointestinal Tract: From the Endoscopists' Viewpoint

    Directory of Open Access Journals (Sweden)

    Chen-Shuan Chung

    2013-01-01

    Full Text Available Upper gastrointestinal tract cancers are one of the most important leading causes of cancer death worldwide. Diagnosis at late stages always brings about poor outcome of these malignancies. The early detection of precancerous or early cancerous lesions of gastrointestinal tract is therefore of utmost importance to improve the overall outcome and maintain a good quality of life of patients. The desire of endoscopists to visualize the invisibles under conventional white-light endoscopy has accelerated the advancements in endoscopy technologies. Nowadays, image-enhanced endoscopy which utilizes optical- or dye-based contrasting techniques has been widely applied in endoscopic screening program of gastrointestinal tract malignancies. These contrasting endoscopic technologies not only improve the visualization of early foci missed by conventional endoscopy, but also gain the insight of histopathology and tumor invasiveness, that is so-called optical biopsy. Here, we will review the application of advanced endoscopy technique in screening program of upper gastrointestinal tract cancers.

  9. Cervical Cancer

    Centers for Disease Control (CDC) Podcasts

    2007-03-06

    Did you know that cervical cancer rates differ by race/ethnicity and region? Or that cervical cancer can usually be prevented if precancerous cervical lesions are found by a Pap test and treated? Find out how getting regular Pap tests can save a woman's life.  Created: 3/6/2007 by National Breast and Cervical Cancer Early Detection Program.   Date Released: 4/25/2007.

  10. Therapeutic vaccination with MVA E2 can eliminate precancerous lesions (CIN 1, CIN 2, and CIN 3) associated with infection by oncogenic human papillomavirus.

    Science.gov (United States)

    Corona Gutierrez, Carlos Manuel; Tinoco, Alberto; Navarro, Tania; Contreras, Mario López; Cortes, Roberto Risco; Calzado, Patricia; Reyes, Lise; Posternak, Roberto; Morosoli, Gianni; Verde, Mauro Lara; Rosales, Ricardo

    2004-05-01

    Human papillomavirus (HPV) infection is associated with cervical cancer. Papillomaviruses can induce diseases ranging from warts and condylomata to lesions that can progress to malignant neoplasias. Cervical cancer is a serious problem in developing countries because it is usually not detected at an early stage. In Mexico, a woman dies every 2 hr from this malignancy. In a phase I/II clinical trial, we evaluated the potential use of the MVA E2 recombinant vaccinia virus to treat cervical intraepithelial neoplasia CIN 1, CIN 2, and CIN 3 lesions associated with human papillomavirus (HPV) infection. Seventy-eight women with CIN 1-, CIN 2-, and CIN 3-grade lesions were treated with either an MVA E2 recombinant virus vaccine or with cryosurgery. Thirty-six women received the recombinant virus vaccine at a total of 10(7) MVA E2 virus particles injected directly into the uterus once every week over a 6-week period. Forty-two patients were treated with cryosurgery. Reduction of lesions was monitored weekly by colposcopy and cytologic analysis. The type of immune response after MVA E2 injection was determined by measuring antibody titers against MVA E2 virus and the E2 protein, and by the presence of cytotoxic activity against cancer cells bearing papillomavirus DNA. The presence of papillomavirus was determined by with the hybrid capture method. Thirty-four of 36 patients showed complete elimination of precancerous lesions after treatment with the MVA E2 vaccine. In two patients, precancerous lesions were reduced from grade CIN 3 to CIN 1. Three other patients presented isolated koilocytes after treatment with MVA E2. Colposcopy revealed no lesions in 85% of patients, and only small aceto-white spots were detected in 15% of patients after treatment with MVA E2. All patients developed antibodies against the MVA E2 vaccine, and vaccination generated a specific cytotoxic response against HPV-transformed cells. Furthermore, 50% of patients showed no evidence of papillomavirus

  11. Detection and Localization of Pre-Cancerous Lesions and Early Lung Cancer Using Tissue Autofluorescence.

    Science.gov (United States)

    Hung, Jaclyn Yip-Chan

    In this work, two different yet related hypotheses were tested by experimental means as follows: (i) pre-cancerous and non-invasive (early) lung cancer can be detected and localized using the fluorescence properties of tumour localizing drugs at non-photosensitizing doses to skin tissue; (ii) significant differences exist in laser-induced autofluorescence between normal, pre-cancerous and cancerous tissues such that these differences alone can be exploited to detect and delineate early lung cancer without using exogenous drug(s). Exogenous fluorescent tumour markers such as hematoporphyrin derivatives (e.g. Photofrin) have been used to enhance to detection of occult lung lesions. Photofrin is preferentially retained in tumor tissues compared to the surrounding normal tissues; it fluoresces at 630 nm and 690 nm when excited at -405 nm. Based on this principle several imaging and non-imaging devices have been developed. However, wider clinical applications were limited due to the skin photosensitivity property of Photofrin. We have postulated that this could be solved by employing a much lower dose of Photofrin (0.25 mg/kg) which was believed to be less photosensitizing to human patients. This postulate was experimentally tested by ratio fluorometry and early lung cancers were detected with no false negative results and no apparent skin photosensitivity. An important finding in this study was that the mechanism for detection of early cancer was mainly due to the differences in the green autofluorescence between normal and malignant tissues, rather than fluorescence of tumour localizing drug. This discovery led to the second postulate of this thesis that tissue autofluorescence alone can be exploited for the detection of early lung cancer. The results indicated that algorithm(s) could be developed to clearly delineate early lesions from the normal tissues. Several algorithms were then tested using a non-imaging ratio fluorometer device and a prototype imaging

  12. An atlas of subgross pathology of the human breast with special reference to possible precancerous lesions.

    Science.gov (United States)

    Wellings, S R; Jensen, H M; Marcum, R G

    1975-08-01

    DCIS, the unfolded lobule resembled a duct which gave the false impression that DCIS was a ductal lesion. The morphologic evidence supported that hypothesis that the lesions herein called AL were derived from TDLU and were precancerous. PMID:169369

  13. Os Odontoideum: Rare Cervical Lesion

    Directory of Open Access Journals (Sweden)

    Kristie A Robson

    2011-05-01

    Full Text Available We report the case of a 22-year-old Marine who presented to the emergency department, after a martial arts exercise, with transient weakness and numbness in all extremities. Computed tomography cervical spine radiographs revealed os odontoideum. Lateral flexion–extension radiographs identified atlanto-axillary instability. This abnormality is rare and can be career ending for military members who do not undergo surgical fusion. [West J Emerg Med. 2011;12(4:520–522.

  14. Prevention of precancerous colonic lesions in rats by soy flakes, soy flour, genistein, and calcium.

    Science.gov (United States)

    Thiagarajan, D G; Bennink, M R; Bourquin, L D; Kavas, F A

    1998-12-01

    The main purpose of this research was to determine whether diets containing soy products would inhibit the early stages of azoxymethane-induced colon cancer in F344 rats. Additional objectives were to determine whether feeding starch instead of sucrose, feeding additional calcium (0.5% compared with 0.1%), or feeding a low-fiber powdered enteral formula would influence early colon carcinogenesis. Colon cancer was initiated with 2 injections of azoxymethane (15 mg/kg body wt) and a 12-wk dietary treatment period was started 1 wk after the second injection. Precancerous colon lesions were assessed as foci with aberrant crypts (FAC). The mean numbers of FAC were 133 [soy concentrate (low concentration of phytochemicals)], 111 (starch substituted for sucrose), 98 [full-fat soy flakes (whole soybeans)], 87 (defatted soy flour), 77 (0.015% genistein), and 70 (0.5% Ca). The soy flour and full-fat soy flake diets contained 0.049% genistein derivatives (primarily glycosides), but were less effective in inhibiting the formation of FAC than the diet containing 0.015% genistein (as the aglycone). Eating soybeans and soy flour may reduce the early stages of colon cancer. PMID:9848506

  15. Prevalence of oral cancer and pre-cancerous lesions and the association with numerous risk factors in North India: A hospital based study

    OpenAIRE

    Gupta, Shalini; Singh, Rajender; O P Gupta; Tripathi, Anurag

    2014-01-01

    Background: Oral cancer is one of the most common life-threatening diseases all over the world. Developing countries face several challenges to identify and remove potential risk factors. Chewing tobacco/pan masala is considered to be the most potent risk factor for oral precancerous lesions and oral cancer. Objectives: To investigate the relative occurrence of different oral pre-cancerous lesions and oral cancer in North India and to identify the associated risk factors. Materials and Method...

  16. Photodynamic therapy for cervical lesions

    Directory of Open Access Journals (Sweden)

    E. V. Grebenkina

    2014-01-01

    Full Text Available The experience of treatment for precancer and early cervical cancer by photodynamic therapy in 12 patients with primary diagnosis H-SIL (CIN II–III and cancer in situ is described. Chlo-rine photosensitizer Photolon was given intravenously at a dose of 0.75–1.15 mg/kg body weight. 2.5 h later the treatment with polyposition laser exposure (light dose – 150 J/cm2, light power density – 400–500 mW/cm2 was made. Thirty days later conization of the cervix with endocervical curettage assessing therapeutic response of cervical tumor tissue was per-formed. According to histological data complete response was in 4 patients, minute foci of CIN I were determined in 7 patients, 1 patient had foci of CIN II. 8 of 10 HPV-positive patients had complete eradication of HPV after treatment. There were no serious adverse events after light exposure. Marked therapeutic response, high anti-viral activity and good feasibility allow to consider photodynamic therapy as alternative organ-sparing treatment of early cancer and pre-cancer of cervix. 

  17. Immunohistochemical expression of MIB-1 and PCNA in precancerous and cancerous lesions of uterine cervix

    Directory of Open Access Journals (Sweden)

    Madhu Mati Goel

    2013-01-01

    Full Text Available Background and Objective: The present study was done to analyze the immunoexpression of diagnostic markers (MIB-1: molecular immunology borstel and PCNA: proliferating cell nuclear antigen in grading cervical intraepithelial lesion (CIN and squamous cell carcinoma (SCC in cervix. Setting and Design: Total 150 cervical biopsies were divided into four groups respectively; Group I-Normal (n = 32, Group II- CIN (n = 60, Group III- SCC (n = 44, Group IV- CA cervix (n = 14 respectively. Materials and Methods: These biopsies were stained with monoclonal antibodies by streptavidin--biotin method. Mean labeling index was calculated and grading was performed using the I--III scoring system. Statistical Analysis: Findings were correlated with age and menopausal status. Statistical analysis was done by using student sample′t′ test and analysis of variance (ANOVA by SPSS 10 package. Results: MIB-1 immunostaining was positive in 112/150 (74.6% cases and PCNA in 118 /150 (78.6% cases. Labeling indices showed linear progression from normal to CIN to SCC to cancer lesion. Few cases of low-grade CIN lesion had high proliferative index. A significant positive correlation was found between age and PCNA and MIB-1 values (P < 0.05 when comparison was made for all the cases. Conclusion: These markers may be useful in identifying low-grade CIN lesion with high proliferative index. These cases should be kept for follow up studies so that proper intervention can be taken at an early stage. This method is simple and cost effective and can easily be done in formaline-fixed paraffin embedded tissues in a clinical laboratory for grading CIN and SCC lesions in cervix.

  18. Positive diagnostic values and histological detection ratios from the Rotterdam cervical cancer screening programme

    NARCIS (Netherlands)

    F.A. Kreuger; H. Beerman (Henk); H.G. Nijs (Huub); M. van Ballegooijen (Marjolein)

    1998-01-01

    textabstractBACKGROUND: In organized screening programmes for cervical cancer, pre-cancerous lesions are detected by cervical smears. However, during follow-up after a positive smear these pre-cancerous lesions are not always found. The purpose of the study is to analys

  19. Assessment of Hemodynamics in a Rat Model of Liver Cirrhosis with Precancerous Lesions Using Multislice Spiral CT Perfusion Imaging

    Directory of Open Access Journals (Sweden)

    Guolin Ma

    2013-01-01

    Full Text Available Rationale and Objectives. To develop an optimal scanning protocol for multislice spiral CT perfusion (CTP imaging to evaluate hemodynamic changes in liver cirrhosis with diethylnitrosamine- (DEN- induced precancerous lesions. Materials and Methods. Male Wistar rats were randomly divided into the control group (n=80 and the precancerous liver cirrhosis group (n=40. The control group received saline injection and the liver cirrhosis group received 50 mg/kg DEN i.p. twice a week for 12 weeks. All animals underwent plain CT scanning, CTP, and contrast-enhanced CT scanning. Scanning parameters were optimized by adjusting the diatrizoate concentration, the flow rate, and the delivery time. The hemodynamics of both groups was further compared using optimized multislice spiral CTP imaging. Results. High-quality CTP images were obtained with following parameters: 150 kV; 150 mAs; 5 mm thickness, 5 mm interval; pitch, 1; matrix, 512×512; and FOV, 9.6 cm. Compared to the control group, the liver cirrhosis group had a significantly increased value of the hepatic arterial fraction and the hepatic artery perfusion (P<0.05 but significantly decreased hepatic portal perfusion and mean transit time (P<0.05. Conclusion. Multislice spiral CTP imaging can be used to evaluate the hemodynamic changes in the rat model of liver cirrhosis with precancerous lesions.

  20. HPV阴性的宫颈癌和癌前病变中p14ARF蛋白表达及其临床意义%Expression and clinical significance of p14ARF protein in HPV - negative patients with cervical cancer and cervical precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    尹香花; 苏悦; 隋玉梅; 成艳

    2011-01-01

    Objective:To explore the relationship between pl4ARF protein, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and early diagnosis, invasion and metastasis of cervical cancer by detecting their expression levels in cervical canc-er and cervical intraepithelial neoplasia (CIN) of human papillomavirus ( HPV ) - negative patients. Methods: 30 HPV - negative patients with chronic cervicitis, 35 HPV - negative patients with CIN and 35 HPV - negative patients with cervical invasive cancer were selected as study ob-jects by PCR. Immunohistochemical SP staining was used to detect the expression level of pl4ARF protein, clinicopathological characteristics and the correlation with the depth of invasion and lymph node metastasis. Results :P14ARF protein specifically expressed in the nuclei and cyto-plasm of CIN and cervical cancer cells, and it never expressed in normal squamous epithelium and glandular epithelium, there was no correla-tion between P14ARF protein expression and clinical stages, while it was related to the degree of differentiation and lymph node metastasis. Conclusion: pl4ARF protein detection can be used as an index for early diagnosis of cervical cancer among HPV - negative patients, which also can be used as an index to predict the potential of invasion and metastasis of cervical cancer and clinical prognosis.%目的:通过检测人乳头状瘤病毒(HPV)感染阴性的官颈癌和宫颈上皮内瘤变中p14ARF和基质金属蛋白酶MMPs及其抑制物TIMPs的表达,探讨其与官颈癌早期诊断、侵袭转移的关系.方法:以PCR方法筛查HPV阴性的慢性官颈炎30例、官颈上皮内瘤变(CIN) 35例及官颈浸润癌35例作为研究对象,采用SP染色法检测p14ARF蛋白在其中的表达,分析p14ARF蛋白在官颈癌组织中的表达、临床病理特征及其与浸润深度、淋巴结转移的相关性.结果:p14ARF蛋白特异性表达在HPV阴性的CIN病变、宫颈癌细胞核及胞质中,在

  1. Diaphragm paralysis from cervical disc lesions.

    Science.gov (United States)

    Cloward, R B

    1988-01-01

    An opera singer, who "made her living with her diaphragm", developed a post-traumatic unilateral radiculopathy due to cervical disc lesions, C3 to C6. During one year of severe neck and left arm pain she gradually lost the ability to sing difficult operatic passages which brought an end to her music career. Following a three level anterior cervical decompression and fusion, the neck and arm pain was immediately relieved. One week later her voice and singing ability returned to its full strength and power permitting her to resume her activities as a vocalist. The diagnosis of paresis of the left hemi-diaphragm as part of the cervical disc syndrome was implied by postoperative retrospective inference.

  2. Qualitative and Quantitative Studies of Polygene Proteins Expression in Esophageal Precancerous Lesions and Esophageal Carcinoma

    Institute of Scientific and Technical Information of China (English)

    LI Chao-xia; WU Ming-yao; KUANG Li-ping

    2007-01-01

    Objective: To examine the expressions of MDM2, P53 and P27 proteins in chronic esophagitis, para-cancer mucosa and esophageal carcinoma. Methods: Immunohistochemistry was used to detect the expressions of MDM2, P53 and P27 proteins in forty-seven patients suffering from chronic esophagitis and eighty-five cases of esophageal carcinoma and corresponding para-cancer mucosa. Flow cytometry((FCM) was applied to detect the quantities of these proteins expressed in fresh tissues of 48 cases of esophageal cancer and their para-cancer tissues and 24 cases of relative normal mucosa at the surface of cutting edge. Results: Immunohistochemistry results showed that the expressions of the three studied proteins were very similar in the epithelia of chronic esophagitis and para-cancer mucosa (P>0.05). Both the qualitative and quantitative studies displayed that the P53 protein had no expression and its accumulations would appear only in the early stages of esophagus canceration while the MDM2 and P27 proteins had different degrees of expressions in cases of normal esophageal mucosa. MDM2 protein markedly increased in the advanced stages of esophageal canceration. A quantitative study showed that the expression of P27 protein had a linearity of decreasing tendency (F=9.132,P=0.002) in the course of esophageal canceration. Conclusion: Chronic esophagitis may be a precancerous lesion. Owing to the changes of the P53 and P27 proteins, we can also conclude that these occur in the early stages of esophagus oncogenesis, however the changes of MDM2 expression may occur in the advanced stage of esophageal canceration.

  3. Expression of E-cadherin in normal oral mucosa, in oral precancerous lesions and in oral carcinomas

    OpenAIRE

    Sridevi, Ugrappa; Jain, Ajay; Nagalaxmi, Velpula; Kumar, Ugrappa Vijay; Goyal, Stuti

    2015-01-01

    Objective: The aim of the present study was to assess the expression of E-cad in oral precancerous lesions and conditions and oral carcinomas in comparison with normal mucosa. Materials and Methods: Total of 50 samples were selected for the study and were categorized into five groups and 10 samples in each group as Group I-oral leukoplakia (OL), Group II-oral lichen planus (OLP), Group III-oral submucous fibrosis (OSMF), Group IV-oral squamous cell carcinoma (OSCC) and Group V-normal oral muc...

  4. Regulative Effect of Traditional Chinese Medicine on Gene-expression Related to Precancerous Lesion of Gastric Cancer

    Institute of Scientific and Technical Information of China (English)

    ZHU Fang-shi; SI Jian-min

    2005-01-01

    The gene-expression changes related with precancerous lesion of gastric cancer (PLGC) are surveyed. Not only the regulative effect of traditional Chinese medicine (TCM) on oncogene, antioncogene and anti-apoptosis gene that are related with PLGC is analyzed, but also current research state is presented. It's showed that TCM has effects of therapy and inversion on PLGC. These effects are related with the inhibition to related oncogene expression, the regulation and activation to the deletion of antioncogene, the inhibition to the high-expression of mutant gene-protein about antioncogene, and the regulative function to anti-apoptosis gene.

  5. Low resource screening method of pre-cancerous lesions and its reversal by Triphala in teen-age Indian population

    OpenAIRE

    Deshpande, Anshula; Tandon, Shobha; Deshpande, Neeraj

    2014-01-01

    Background: Cancer screening is the main weapon for early detection at a pre-invasive or premalignant stage. It has been reported that over 12 million people use some form of tobacco, which is one of the high risk factors and has hence become an alarming world-wide problem. Aim: To evaluate the effective diagnostic screening of disease in its early stage by inexpensive method and also to evaluate the effect of indigenous mouthrinse on reversal of pre-cancerous lesions. Materials and Methods: ...

  6. Epigenetic silencing of monoallelically methylated miRNA loci in precancerous colorectal lesions.

    Science.gov (United States)

    Menigatti, M; Staiano, T; Manser, C N; Bauerfeind, P; Komljenovic, A; Robinson, M; Jiricny, J; Buffoli, F; Marra, G

    2013-01-01

    Epigenetic silencing of protein-encoding genes is common in early-stage colorectal tumorigenesis. Less is known about the methylation-mediated silencing of genes encoding microRNAs (miRNAs), which are also important epigenetic modulators of gene expression. Using quantitative PCR, we identified 56 miRNAs that were expressed in normal colorectal mucosa and in HT29 colorectal cancer cells treated with demethylating agents but not in untreated HT29 cells, suggesting that they probably undergo methylation-induced silencing during colorectal tumorigenesis. One of these, miR-195, had recently been reported to be underexpressed in colorectal cancers and to exert tumor-suppressor effects in colorectal cancer cells. We identified the transcription start site (TSS) for primary miRNA (pri-miR)-497/195, the primary precursor that yields miR-195 and another candidate on our list, miR-497, and a single CpG island upstream to the TSS, which controls expression of both miRNAs. Combined bisulfite restriction analysis and bisulfite genomic sequencing studies revealed monoallelic methylation of this island in normal colorectal mucosa (50/50 samples) and full methylation in most colorectal adenomas (38/50; 76%). The hypermethylated precancerous lesions displayed significantly downregulated expression of both miRNAs. Similar methylation patterns were observed at two known imprinted genes, MEG3 and GNAS-AS1, which encode several of the 56 miRNAs on our list. Imprinting at these loci was lost in over half the adenomas (62% at MEG3 and 52% at GNAS-AS1). Copy-number alterations at MEG3, GNAS-AS1 and pri-miR-497/195, which are frequent in colorectal cancers, were less common in adenomas and confined to tumors displaying differential methylation at the involved locus. Our data show that somatically acquired, epigenetic changes at monoallelically methylated regions encoding miRNAs are relatively frequent in sporadic colorectal adenomas and might contribute to the onset and progression of

  7. Inhibition of precancerous lesions development in kidneys by chrysin via regulating hyperproliferation, inflammation and apoptosis at pre clinical stage.

    Science.gov (United States)

    Rashid, Summya; Nafees, Sana; Vafa, Abul; Afzal, Shekh Muhammad; Ali, Nemat; Rehman, Muneeb U; Hasan, Syed Kazim; Siddiqi, Aisha; Barnwal, Preeti; Majed, Ferial; Sultana, Sarwat

    2016-09-15

    Chrysin (CH) is natural, biologically active compound, belongs to flavoniod family and possesses diverse pharmacological activities as anti-inflammatory, anti-oxidant and anti-cancer. It is found in many plants, honey and propolis. In the present study, we investigated the chemopreventive efficacy of CH against N-nitrosodiethylamine (DEN) initiated and Fe-NTA induced precancerous lesions and its role in regulating oxidative injury, hyperproliferation, tumor incidences, histopathological alterations, inflammation, and apoptosis in the kidneys of Wistar rats. Renal cancer was initiated by single intraperitoneal (i.p.) injection of DEN (200 mg/kg bw) and promoted by twice weekly injection of ferric nitrilotriacetate (Fe-NTA) 9 mg Fe/kg bw for 16 weeks. CH attenuated Fe-NTA enhanced renal lipid peroxidation, serum toxicity markers and restored renal anti oxidant armory significantly. CH supplementation suppressed the development of precancerous lesions via down regulation of cell proliferation marker like PCNA; inflammatory mediators like TNF-α, IL-6, NFkB, COX-2, iNOS; tumor incidences. CH up regulated intrinsic apoptotic pathway proteins like bax, caspase-9 and caspase-3 along with down regulation of Bcl-2 triggering apoptosis. Histopathological and ultra structural alterations further confirmed biochemical and immunohistochemical results. These results provide powerful evidence for the chemopreventive efficacy of CH against chemically induced renal carcinogenesis possibly by modulation of multiple molecular pathways. PMID:27403965

  8. EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR AND HUMAN PAPILLOMAVIRUS (HPV L1 CAPSID PROTEIN IN CERVICAL SQUAMOUS INTRAEPITHELIAL LESIONS

    Directory of Open Access Journals (Sweden)

    Balan Raluca

    2010-09-01

    Full Text Available We analyzed the immunohistochemical pattern of epidermal growth factor receptor (EGFR in cervical squamous intraepithelial lesions (SILs in correlation with L1 HPV capsid protein, in order to determine the relationship between EGFR expression and the infection status of human papillomavirus (HPV. The study included 40 cases, 24 LSIL (low grade SIL (CIN1, cervical intraepithelial neoplasia and 16 HSIL (high grade SIL (6 cases of CIN2 and 10 cases of CIN3. The immunoexpression of L1 HPV protein was assessed on conventional cervico-vaginal smears and EGFR was immunohistochemically evaluated on the corresponding cervical biopsies. The HPV L1 capsid protein was expressed in 45.83% of LSIL and 25% of HSIL. EGFR was overexpressed in 62,4% of HSIL (58,4% CIN2 and 41,6% CIN3 and 37,6% LSIL. The immunoexpression of L1 HPV has clinical application in the progression assessment of the cervical precancerous lesions without a correlation to the grade of the cervical SIL. EGFR is expressed by all proliferating squamous epithelial cells, thus corresponding with the grade of SIL. The evaluation of EGFR status, correlated with L1 HPV protein expression, can provide useful data of progression risk of cervical squamous intraepithelial lesions

  9. Expression of gastric cancer—associated MG7 antigen in gastric cancer,precancerous lesions and H.pylori—associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Dong-LiGuo; MingDong; LanWang; Li-PingSun; YuanYuan

    2002-01-01

    AIM:To investigate the relationship between the antigen MG7 antigen expression and gastric cancer as well as precancerous condition;to study the relationship between the MG7 antigen expression and H.pyiori infection in benign gastric lesions in order to find out the effect of H.pylori infection on the process of gastric cancer development.

  10. Effects of San Qi on Gastric Secretion and Protective Factors of Gastric Mucosa in the Rat with Precancerous Lesion of Stomach

    Institute of Scientific and Technical Information of China (English)

    石雪迎; 赵凤志; 戴欣; 董秀云; 方杰; 杨会敏

    2003-01-01

    @@ In the model rat with precancerous lesion of stomach induced by the combined method of insertion of a spring into the pylorus and high salt hot paste, effects of San Qi (三七 Radix Notoginseng) on gastric secretion and protective factors of stomach were investigated.

  11. Serum vitamin D levels of patients with oral squamous cell carcinoma (OSCC) and expression of vitamin D receptor in oral precancerous lesions and OSCC

    OpenAIRE

    Grimm, Martin; Cetindis, Marcel; Biegner, Thorsten; Lehman, Max; Munz, Adelheid; Teriete, Peter; Reinert, Siegmar

    2015-01-01

    Background: Resistance to programmed cell death (apoptosis) is a crucial factor for the carcinogenesis of oral squamous cell carcinoma (OSCC). Vitamin D (calcitriol) may overcome apoptosis resistance in tumor cells of OSCC. Vitamin D receptor (VDR) expression in oral precancerous lesions of OSCC has not been analyzed and serum vitamin D level seems to be a predictor of cancer development. Material and Methods: Expression of VDR was analyzed in normal oral mucosa (n=5), oral precursor lesions ...

  12. The Value of DNA Ploidy Technology in Cervical Cancer and Precancerous%DNA倍体技术在宫颈癌和癌前病变筛查中的价值探讨

    Institute of Scientific and Technical Information of China (English)

    刘晓艳

    2014-01-01

    Objective:To investigate the value of DNA ploidy technology in cervical cancer and precancerous. Method:1538 cases were be screened,the first pathological biopsy performed after DNA ploidy analysis,then differences between the DNA ploidy and biopsy examination were compared.Result:The samples were drawn from 1538 cases,DNA ploidy negative analysis was 1425 cases,negative rate was 92.7%,the highest positive rate was over 51 years old and followed by 31 to 40 years old,pathological diagnosis of cervical cancer or precancerous lesions was 55 cases,DNA ploidy analysis missed the presence of two cases,the diagnosis rate was 96.4%.Conclusion:DNA ploidy analysis and screening for early diagnosis of cervical cancer and precancerous lesions,the diagnostic accuracy rate is high,it can easily obtain and check the simple non-invasive,so it is a more reliable screening method.%目的:探讨DNA倍体技术在宫颈癌和癌前病变筛查中的价值。方法:选取1538例待筛查者,先进行DNA倍体分析之后进行病理活检检查,比较DNA倍体分析结果与病理活检检查的差异。结果:本组共取材1538例样本,DNA倍体分析阴性者1425例,阴性率92.7%,51岁及以上阳性率最高,其次为31~40岁者,病理检查确诊宫颈癌或者癌前病变者55例,DNA倍体分析存在2例漏诊,其诊断率达到96.4%。结论:DNA倍体分析对于宫颈癌及其癌前病变的早期诊断及筛查,诊断符合率高,取材方便,检查简单无创,是一种较为可靠的普查方法。

  13. Human Papillomavirus Genotyping and p16INK4a Expression in Cervical Lesions: A Combined Test to Avoid Cervical Cancer Progression

    Science.gov (United States)

    Zouheir, Yassine; Fechtali, Taoufiq; Elgnaoui, Nadia

    2016-01-01

    Cervical cancer is a major public health problem in Morocco. The cervical cancer has a long precancerous period that provides an opportunity for the screening and treatment. Improving screening tests is a priority goal for the early diagnosis of cervical cancer. This study was conducted to evaluate the combination of p16INK4a protein expression, human papillomavirus (HPV) typing, and histopathology for the identification of cervical lesions with high risk to progress to cervical cancer among Moroccan women. A total of 96 cervical biopsies were included in this study. Signal amplification in situ hybridization with biotinylated probes was used to detect HPV. Immunohistochemistry was used to evaluate the expression of p16INK4a protein. HPV DNA was detected in 74.0% of the biopsies (71/96). Of the seventy-one positive HPV cases, we detected 67.6% (48/71) of high risk (HR)-HPV (HPV 16 and 18), 24% of low risk-HPV (HPV 6 and 11), 1.4% intermediate risk-HPV (HPV 31, 33, and 35), and 7% coinfections (HPV 6/11 and 16/18). Overexpression of p16INK4a protein was observed in 72.9% (70/96) of the biopsies. In addition, p16INK4a protein detection was closely correlated with recovery of HR HPV. Our result showed that p16INK4a expression level is correlated with HR-HPV status. PMID:27390742

  14. Human Papillomavirus and Cervical Cancer

    OpenAIRE

    D. Jenkins(University of York, UK)

    2003-01-01

    Of the many types of human papillomavirus (HPV), more than 30 infect the genital tract. The association between certain oncogenic (high-risk) strains of HPV and cervical cancer is well established. Although HPV is essential to the transformation of cervical epithelial cells, it is not sufficient, and a variety of cofactors and molecular events influence whether cervical cancer will develop. Early detection and treatment of precancerous lesions can prevent progression to cervical cancer. Ident...

  15. The Prevalence of Human Papilloma Virus(HPV in Malignant Cervical Lesion, Using Multiplex PCR

    Directory of Open Access Journals (Sweden)

    M. R. Keyhkhaee

    2006-07-01

    Full Text Available Background: Cervical cancer is the second leading cause of cancer death among women. In this cancer, the effects of prevention, early diagnosis and treatment more than other cancers decrease the mortality rate. In 1970 human papilloma virus (HPV was introduction as major etiologic factor of cervical cancer. Different studies throughout the world revealed strong correlation between HPV and cancerous & precancerous changes in epithelial cells. Since cell culture and serological methods can not recognize the virus and its subtypes, the importance of the molecular methods including polymerase chain reaction (PCR in early and definite diagnosis of virus is obvious. Methods: In this study, after patient selection using the related protocol and completion of the questionnaires, 100 samples from cancer lesions of cervix selected. Then DNA extraction from paraffin blocks performed using standard method. Multiplex PCR with two pairs of primer (one as internal control performed and the PCR product run on 8% polyacrylamid gel. Results: The results showed that 73% of the tissues were infected by HPV. Conclusion: This finding confirm the previous results based of correlation between HPV,and cervical cancer.

  16. Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas

    International Nuclear Information System (INIS)

    Human papillomavirus type 16 (HPV16) genome DNA and its transcripts in biopsied cervical neoplasias were analyzed by simultaneous extraction of DNA and RNA from one biopsied sample. Southern blot analysis revealed that 5 of 20 cervical intraepithelial neoplasias (CINs) contained HPV16 DNAs existing primarily as episomes and two of seven invasive carcinomas harbored HPV16 genome sequences integrated into the host DNA. Northern (RNA) blot analysis showed that the HPV16 genome sequences were transcriptionally active in the five CINs, as well as in the two invasive carcinomas. The pattern of HPV16-specific transcripts in the CINs was uniform, and the major transcripts were 4.2, 2.2, 1.6, and 1.4 kilobases in size. However, the pattern of HPV16-specific transcripts in the invasive carcinomas was variable and different from that in CINs, suggesting that the alteration of transcriptional pattern might play a key role in the development of malignancy

  17. Diagnostic tests for the detection of human papillomavirus-associated cervical lesions.

    Science.gov (United States)

    Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-01-01

    Current diagnostic approaches for primary cervical cancer screening, work-up of equivocal or positive screening results or follow- up after treatment of precancerous lesions primarily rely on the morphologic interpretation of squamous epithelial cells (Pap cytology), in some setting accompanied by the detection of human papillomavirus DNA and have largely contributed to remarkable reduction of disease incidence in countries with implemented screening programs. However, these approaches are limited by a poor sensitivity and reproducibility of Pap cytology and low specificity for high grade cervical intraepithelial neoplasia of HPV DNA detection assays. Early detection might be improved by complementing or even replacing these tests by markers which are more directly related to molecular events triggering HPV-induced carcinogenesis and thereby might deliver more accurate diagnostic performance. The delineation of molecular changes which occur during different stages of HPV infections and the identification of changes which induce neoplastic alterations allow for the detection of markers that specifically highlight the transforming stage of the infection where viral oncogenes are overexpressed and therefore allow for a more specific diagnosis of lesions that require treatment. The evaluation of such markers in clinical studies revealed that some indeed show an improved diagnostic performance compared to Pap cytology or HPV DNA tests only.

  18. Associated factors with cervical pre-malignant lesions among the married fisher women community at Sadras, Tamil Nadu

    Directory of Open Access Journals (Sweden)

    Sornam Ganesan

    2015-01-01

    Full Text Available Objective: To identify the associated factors of cervical pre-malignant lesions among the married fisher women residing in the coastal areas of Sadras, Tamil Nadu. Methods: The study was conducted in five fishermen communities under Sadras, a coastal area in Tamil Nadu, India. Two hundred and fifty married fisher women residing in the area. Quantitative descriptive approach with a cross-sectional study design was used. Data were collected using a structured interview schedule for identifying the associated factors and Pap smear test was performed for identifying the pre-malignant cervical lesions among the married fisher women. Data were analyzed using descriptive and inferential statistics. Results: Among 250 women, about six (2.4% of them presented with pre-cancerous lesions such as atypical squamous cell of undifferentiated significance (ASCUS - five (2% and mild dysplasia one (0.4%. Majority of the women, about 178 (71.2% women, had abnormal cervical findings. Statistical analysis showed a significant association of risk factors such as advanced age, lack of education, low socioeconomic status, using tobacco, multiparity, premarital sex, extramarital relationship, using cloth as sanitary napkin, etc. Conclusion: The study findings clearly show the increased vulnerable state of the fisher women for acquiring cervical cancer as they had many risk factors contributing to the same.

  19. Expression of HIF-1α in breast cancer and precancerous lesions and the relationship to clinicopathological features

    Institute of Scientific and Technical Information of China (English)

    Yun’ai Liang; Zengxin Li; Gangping Wang

    2014-01-01

    Objective: The aim of this study was to observe the expressions and clinical significance of HIF-1α in breast cancer and precancerous lesions, and analyze the relationship between the expressions and clinicopathological features in breast cancer. Methods: We analyzed the HIF-1α expression in 128 cases of invasive ductal carcinomas, 146 precancerous lesions patients including 89 cases of ductal carcinoma in situ and 57 cases of atypical ductal hyperplasia. 53 cases of usual ductal hyperplasia breast tissues were selected as a control group. The specimens were evaluated for HIF-1α, estrogen re-ceptor (ER) & progesterone receptor (PR), epidermal growth factor receptor type 2 (HER2/neu) and Ki-67. Immunoreactivity was semi-quantitatively evaluated in at least 1000 cells examined under the microscope at 40 × magnification and recorded as the percentage of positive tumor cells over the total number of cells examined in the same area. The percentage scores were subsequently categorized. The express of HIF-1α and their relationship with multiple biological parameters including ER& PR, HER2/neu and Ki-67, the biomarkers levels of CA153, CA125 TSGF, and CEA in blood serum and nipple discharge, histological grade, region lymph node metastasis, distant metastasis and recurrence on files were also assessed. Results:Compared with usual ductal hyperplasia, the positive expression rate of HIF-1α in atypical ductal hyperplasia, ductal carci-noma in situ and invasive ductal carcinomas group was significantly increased (P 14% groups, histological grade (I + II) and grade III invasive ductal carcinomas groups, with lymph node metastasis, distant metastasis and recurrence groups (P50 years), tumor diameter (≤ 2 cm vs > 2 cm; P > 0.05). The nipple discharge and serum levels of CA153, TSGF, CA125 and CEA in invasive ductal carcinomas HIF-1α positive patients were significantly higher than those in the negative patients (P <0.05). Conclusion: In breast cancer, HIF-1α expression

  20. Utility of Papanicolaou test in diagnosis of cervical lesions: a study in a tertiary care centre of western Uttar Pradesh

    Directory of Open Access Journals (Sweden)

    Mithila Bisht

    2015-05-01

    Full Text Available Background: Cervical cancer is the most common cancer among females in India. It is also one of the major causes of deaths among them. Papanicolaou (Pap cytological test helps in detecting the early epithelial abnormalities in cervical cells. The morbidity and mortality due to cervical cancer has come down in countries with well-established screening programmes at national level. This study was conducted to emphasize the value of cervical screening as a tool for early detection of non-neoplastic and neoplastic lesions of cervix. Slide positivity rate was computed and clinico-pathological correlation was done. Methods: This cross-sectional study was conducted from January 2012 to December 2014 (3 years, on 2202 women aged between 20-70 years coming for a Pap smear examination in a tertiary care hospital in Bareilly, India. After staining with conventional Papanicolaou technique, all cases were classified as per Bethesda nomenclature (2001. Results: Out of 2202 cases, 85.16% slides were found to be negative for intraepithelial abnormalities and 14.84% slides were labelled as positive for epithelial lesions. 97.67% of high grade lesions were found in women more than 40 years. Smears with epithelial cell abnormality were found more common in older age groups whereas smears negative for epithelial cell abnormality were found more common in young age groups. Conclusion: Pap test is a cost effective cancer screening and is a simple method to detect various lesions of cervix, non-neoplastic as well as neoplastic. High risk screening programmes should be directed to all women >40 years. Pap test is especially useful to diagnose precancerous lesions of cervix, thereby early detection of these lesions and subsequent proper treatment can be helpful in prevention of cervical cancer. [Int J Res Med Sci 2015; 3(5.000: 1070-1076

  1. Research of cervical cytology screening in diagnosis of 370,000 cases with cervical lesion in Guangdong%广东地区37万例妇女子宫颈细胞学筛查子宫颈病变的研究

    Institute of Scientific and Technical Information of China (English)

    Baowen Zheng; Congde Chen; Anxiang Wei; Han Ran; Dong Liu; Wenling Yue; Huirong Zhou; Qingyi Zhu; Juming Su

    2009-01-01

    Objective:Analysis of the results of 37 million cases by using cervical liquid-based cytology screening.discussion the situation of cervical cancer and precancerous lesions in Guangdong province.Methods:From 2002 to 2006.using liquid-based thin-layer cytology (TCT)method screening cervical lesions within 371.929 women in Guangdong.Cytological diagnosis adopt TBS(the Bethesda system)Standard(TBS improved 2001 version of the diagnostic criteria),staastic positive rate by age and region respectively.Results:371,929 cases of gynecology cervical samples in Guangdong.331,251 cases were negative,accounted for 91.23%:cytology positive cases:32,548 cases were squamous intreepithelial lesions(8.96%),486 cases were glandular epithelial lesions(0.13%):grouped according to age:30 to 40-year-old positive rate was 9.13%,40 to 50-year-old positive rate was 9.60%.the latter had higher cytology positive rate;grouped according to region:the total samples in PRD areas were 304.951 cases,accounted for 81.99%,in which 24301 cases were positive(8.14%),in 66978 cases(18.01%)of NON-PRD regions,7645 cases were positive(11.87%),there were 1858 cases had biopsy/follow-up results from 2004t02005.and the rate was 2.88%.Conclusion:The cytological positive rate of cervical cancer and its pre-cancerous lesions was 9.09%in Guangdong(of which the rate of cytological diagnosis as precancerous lesions was 3.40%),30 to 50 age group had the high incidence of cervical cancer and its precursor lesions.and also the main stage of prevention for cervical cancer.Cytology positive rate was 11.87% in economic underdevelopment Non-Pead River Delta regions.much higher than the Pearl River Delta region.

  2. Evaluation of five screening methods for an early detection of cervical cancer and its precancerous lesions in Zhejiang province%五种检测方法在宫颈癌及其前期病变筛查中的准确性评估

    Institute of Scientific and Technical Information of China (English)

    费华丽; 程易凡; 程晓东; 陈晓端; 叶枫; 吕卫国; 谢幸

    2011-01-01

    目的 比较5种现行的筛查方法在宫颈癌高发地区的应用,从而评价出最佳宫颈癌筛查方案,进而在浙江省范围内推广.方法 在浙江省丽水市某社区内计划选取1005名年龄在25~65岁、有性生活的女性进行宫颈癌筛查,筛查手段选择巴氏涂片、液基细胞学、HPV-DNA检测法、肉眼观察辅以醋酸白染色(VIA)和肉眼观察辅以Lugol's碘染色(VILI)5种.所有筛查者均接受阴道镜检查,以病理组织学结果为金标准,阴道镜检查无异常作为阴性结果.结果 巴氏涂片对宫颈上皮内高度病变的敏感度、特异度、阳性预测值、阴性预测值分别如下:25%,90%,26.7%,98.6%;液基细胞学的上述指标分别为81.3%,97.3%,35.1%,99.6%;VIA上述指标为:68.9%,82.8%,7.1%,99.3%;VILI上述指标分别为:81.3%,84.6%,9.1%,99.6%;HPV-DNA检测法上述指标为:87.5%,77.3%,6.8%,99.7%.结论 液基细胞学的诊断价值好于其余4种方法,可作为宫颈癌筛查的首选方法;肉眼观察由于其简单易行,价格低廉,可适用于经济欠发达地区的普通人群的筛查.%Objective To evaluate five screening methods of cervical cancer so as to popularize an effective screening strategy for cervical cancer in Zhejiang province. Methods A total of 1005 women aged 25 -65 years old were selected from Lishui where cervical cancer was highly prevalent. And 859 subjects were ultimately enrolled between June 2009 and December 2009. Each subject was subjected to five screening methods, including Pap smear, liquid-based cytology (LBC), human papillomavirus DNA with a second-generation hybridization assay( HC2 ), visual inspection with acetic acid (VIA) and visual inspection with Lugol's iodine (VILI). CIN (cervical intraepithelial neoplasia) 2 + on biopsy was used as the reference standard for disease positivity. Negative colposcopy was accepted as a negative outcome. Results The sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative

  3. Loss of heterozygosity on 10q23.3 and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Yi-Ling Li; Zhong Tian; Dong-Ying Wu; Bao-Yu Fu; Yan Xin

    2005-01-01

    AIM: To investigate the loss of heterozygosity (LOH) and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions.METHODS: Thirty cases of normal gastric mucosa, advanced and early stage gastric cancer, intestinal metaplasia, atrophic gastritis, and atypical hyperplasia were analyzed for PTEN LOH and mutations within the entire coding region of PTEN gene by PCR-SSCP denaturing PAGE gel electrophoresis,and PTEN mutation was detected by PCR-SSCP sequencing followed by silver staining.RESULTS: LOH rate found in respectively atrophic gastritis was 10% (3/30), intestinal metaplasia 10% (3/30), atypical hyperpiasia 13.3% (4/30), early stage gastric cancer 20%(6/30), and advanced stage gastric cancer 33.3% (9/30),None of the precancerous lesions and early stage gastric cancer showed PTEN mutations, but 10% (3/30) of the advanced stage gastric cancers, which were all positive for LOH, showed PTEN mutation.CONCLUSION: LOH of PTEN gene appears in precancerous lesions, and PTEN mutations are restricted to advanced gastric cancer, LOH and mutation of PTEN gene are closely related to the infiltration and metastasis of gastric cancer.

  4. Expression of bcl-2 oncogene in gastric precancerous lesions and its correlation with syndromes in traditional Chinese medicine

    Institute of Scientific and Technical Information of China (English)

    Ling Hu; Shao-Xian Lao; Chun-Zhi Tang

    2005-01-01

    AIM: To observe the protein and mRNA expression of bcl-2 oncogene in gastric precancerous lesions (GPL) and to analyze its correlation with syndromes in traditional Chinese medicine (TCM).METHODS: Sixty-seven patients with GPL confirmed by gastroscopy and pathology were studied, including 39 cases of moderate gastric mucosal dysplasia, 19 casesof severe gastric mucosa dysplasia, g cases of incompletecolon metaplasia. In syndrome differentiation of TCM, 17 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by qi stagnation, 21 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by stomach heat, 29 cases belonged to the syndrome of qi and yin deficiency of the spleen and stomach complicated by blood stasis. Protein and mRNA expression of bcl-2 oncogene weredetected by labeled streptavidin biotin (LSAB) immunohistochemistry and in situ hybridization respectively. RESULTS: Abnormal expression of protein and mRNA on bcl-2 oncogene was found in GPL, which increased gradually with the course of lesions. In moderate and severe gastric mucosal dysplasia and incomplete colon metaplasia, there was no difference in the expression of bcl-2 oncogene (P>0.05). In different accompanying syndromes, the expression of protein and mRNA on bcl-2 oncogene increased gradually in the following order: deficiency of both qi and yin of the spleen and stomach accompanying qi stagnation → stomach heat → blood stasis. In GPL, compared with accompanying blood stasis, there was an obvious difference in the expression of bd-2 oncogene between the syndrome of qi and yin deficiency of the spleen and stomach and accompanying stomach heat, so did accompanying qi stagnation (the level of protein: χ2 = 8.45, P<0.05; the level of mRNA: χ2 = 7.35,P<0.05).CONCLUSION: Apoptosis-associated bcl-2 oncogene is abnormally expressed in GPL, which correlates with different accompanying syndromes in TCM.

  5. Analysis of human serum from women affected by cervical lesions.

    Science.gov (United States)

    Barba de la Rosa, Ana P; Lugo-Melchor, Ofelia Y; Briones-Cerecero, Erika P; Chagolla-López, Alicia; De León-Rodríguez, Antonio; Santos, Leticia; Vázquez-Ortiz, Guelaguetza; Salcedo, Mauricio

    2008-01-01

    Cervical cancer is one of the first causes of death in Mexican women population. The plasma proteome has a wide dynamic range concentrations of different protein and their alterations reflect the physiological state of the individual's health. The aim of this study was to characterize the 2D-PAGE serum patterns from healthy women and with different levels of cervical lesions. Changes in haptoglobin, apolipoproteins, and transthyretin, when comparing the serum from healthy women and serum from patients with different levels of cervical lesion were found. The Western blot analysis showed increasing concentrations of metalloproteinases (MMP's), proteins with important biological roles in tumor development and metastasis. Protein profiles in conjunction with MS, bioinformatics, and Western blot analysis, allow us to compile information for the acquisition of results to proposed candidates biomarkers of cervical cancer among Mexican women population.

  6. Randomized Phase II Trial of Lyophilized Strawberries in Patients with Dysplastic Precancerous Lesions of the Esophagus

    Science.gov (United States)

    Chen, Tong; Yan, Fei; Qian, Jiaming; Guo, Mingzhou; Zhang, Hongbing; Tang, Xiaofei; Chen, Fang; Stoner, Gary D.; Wang, Xiaomin

    2016-01-01

    Dysplasia is a histologic precursor of esophageal squamous cell carcinoma (SCC). We previously showed that dietary freeze-dried, or lyophilized, strawberry powder inhibits N-nitrosomethylbenzylamine-induced SCC in the rat esophagus. On the basis of this observation, we conducted a randomized (noncomparative) phase II trial in China to investigate the effects of two doses of freeze-dried strawberries in patients with esophageal dysplastic lesions in a high-risk area for esophageal cancer. We randomly assigned 75 patients identified by endoscopy to have dysplastic esophageal premalignant lesions to receive freeze-dried strawberry powder at either 30 g/d (37 patients) or 60 g/d (38 patients) for six months; the powder was mixed with water and drunk. After six months, we assessed the changes in histologic grade of these lesions (primary endpoint) in a blinded fashion. The dose of 30 g/d, did not significantly affect histology or any other measured parameter. The dose of 60 g/d, however, reduced the histologic grade of dysplastic premalignant lesions in 29 (80.6%) of the 36 patients at this dose who were evaluated for histology (P < 0.0001). The strawberry powder was well tolerated, with no toxic effects or serious adverse events. Strawberries (60 g/d) also reduced protein expression levels of inducible nitric oxide synthase (iNOS) by 79.5% (P < 0.001), cyclooxygenase-2 (COX-2) by 62.9% (P < 0.001), phospho-nuclear factor kappa B (NFκB)-p65 (pNFκB-p65) by 62.6% (P < 0.001), and phospho-S6 (pS6) by 73.2% (P < 0.001). Freeze-dried strawberries (60 g/d) also significantly inhibited the Ki-67 labeling index by 37.9% (P = 0.023). Our present results indicate the potential of freeze-dried strawberry powder for preventing human esophageal cancer, supporting further clinical testing of this natural agent in this setting. PMID:22135048

  7. Imperative role of early diagnosis in precancerous lesions: Oldideas, new findings, yet more questions

    Directory of Open Access Journals (Sweden)

    Puneet Kumar

    2012-01-01

    Full Text Available In India, cancers of the oral cavity and oropharynx are most common of all malignancies. Over 90% of these tumors are squamous cell carcinomas (SCCs, which arise from the oral mucosal lining. In spite of the ready accessibility of the oral cavity to direct examination, these malignancies still are often not detected until a late stage, and the survival rate for oral cancer has remained essentially unchanged over the past three decades. The purpose of this article is to review the clinical features of premalignant oral lesions, with an emphasis on early detection.

  8. Mutagens, N-nitroso compounds and their precursors in gastric juice from patients with and without precancerous lesions of the stomach.

    Science.gov (United States)

    Pignatelli, B; Malaveille, C; Rogatko, A; Hautefeuille, A; Thuillier, P; Muñoz, N; Moulinier, B; Berger, F; De Montclos, H; Lambert, R

    1993-01-01

    This study examined whether elevated risk of gastric cancer is associated with high levels of total N-nitroso compounds (NOC), their precursors and nitrosation-dependent genotoxins in gastric juice (GJ). An improved method for quantifying total NOC was used and genotoxicity was assayed in E. coli. Results from patients (n = 210) with or without precancerous lesions of the stomach and living in three areas with up to 8-fold variations in gastric cancer risk (U.K., France, Colombia) were compared. The level of nitrite (range 4 and highest in samples from Colombia. The results (a) provide no support that intragastric total NOC levels are elevated in subjects with precancerous stomach lesions or living in a high risk area for stomach cancer; (b) confirm that a high nitrite level and elevated pH in GJ are strongly associated, the level of nitrite being associated with precancerous stomach conditions only in Colombia; (c) reveal the presence of precursor compounds in GJ, that after nitrosation yield direct mutagens that probably contain NOC and other substances. As their concentrations were significantly higher in achlorhydric subjects and highest in Colombian patients, these data together provide support for a role of intragastrically formed nitrite-derived direct mutagens in gastric cancer aetiology. PMID:8280498

  9. 分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性%linical Efficacy Analysis of Gynecatoptron and LEEP in the Treatment of Precancerous Lesions of Uterine Cervix

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2015-01-01

    Objective To analyze the effect of gynecatoptron and LEEP knife in the treatment of precancerous lesions of u-terine cervix in clinic. Methods 48 cases of patients diagnosed as cervical precancerous lesions by colposcopy biopsy pathologic examination were selected from 108 cases of vaginitis patients treated in our hospital from March 2015 to August 2015 and they were given LEEP operation by colposcopy, the clinical treatment effect was analyzed. Results by the cervix biopsy, the sensitivity ,specificity, positive predictive values, negative predictive values , misdiagnosis rate and missed diag-nosis rate of HSIL were respectively 80.83%, 91.43%, 76.38%, 92.29%, 6.9% and 10.4%. and 82.45%、90.06%、77.44%、92.54%、5.3% and 10.5% of LSIL successively, the total coincidence probability of cervix biopsy results and pathological tissue diagnosis of LEEP operation was 95.8%, the rate of conversion was 93.75% after 3 months of follow-up and 100%after 6 months of follow-up by LEEP knife treatment. Conclusion The choice of gynecatoptron and LEEP operation in the treatment of patients with precancerous lesions of uterine cervix can greatly reduce trauma and avoid severe bleeding prob-lems, the application effect of which is obvious.%目的:分析分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性。方法从2015年3—8月该院108例阴道镜患者中,整群选择48例经阴道镜活检病理检查诊断为宫颈癌前病变的入院者,给予阴道镜下LEEP手术治疗,分析其临床治疗效果。结果阴道镜的活检结果和LEEP手术的病理组织诊断的总符合概率95.8%;采用LEEP刀治疗,3个月的随访的转阴率为93.75%,6个月转阴率100%。结论阴道镜与LEEP治疗宫颈癌前病变的临床效果显著。

  10. La leucoplasia oral: Su implicación como lesión precancerosa Oral leukoplasia: A precancerous lesion

    Directory of Open Access Journals (Sweden)

    A. Martínez-Sahuquillo Márquez

    2008-02-01

    Full Text Available La leucoplasia es definida actualmente en base a un concepto esencialmente clínico. En este sentido, se propone un diagnóstico provisional de leucoplasia basado en criterios clínicos y un diagnóstico definitivo para el que se requiere la eliminación de los factores etiológicos y, ante lesiones persistentes, el examen histopatológico. La consideración de la leucoplasia como lesión precancerosa proviene esencialmente de los estudios de seguimiento en los que se constata su transformación maligna. Pese a que el diagnóstico de displasia es esencialmente subjetivo y a la existencia de algún trabajo reciente que no encuentra relación entre ésta y la evolución posterior de las leucoplasias, la presencia de displasia epitelial en el examen histológico se considera actualmente como el indicador más importante de una mayor probabilidad de transformación maligna de la leucoplasia. Entre otros factores relacionados con un peor pronóstico se consideran el que se trate de una leucoplasia idiopática, las formas clínicas no homogéneas, la localización en cara ventrolateral de la lengua y suelo de boca y un tamaño mayor de 1 centímetro.The current definition of leukoplakia is based on an essentially clinical concept. Therefore, both a provisional diagnosis of leukoplakia based on clinical criteria and a definite diagnosis for which etiological factors need to be eliminated and, should there be persistent lesions, a histological examination is required, are accepted. Follow up studies in which a malignant transformation has been observed have resulted in leukoplakia being considered as a precancerous disorder. Despite the fact that the diagnosis of dysplasia is subjective and certain recent studies have not found any association between this and the development of leukoplakia, the presence of epithelial dysplasia in the histological examination is currently considered to be the most important indicator of a higher possibility of malignant

  11. TTV and HPV co-infection in cervical smears of patients with cervical lesions

    Directory of Open Access Journals (Sweden)

    Tachezy Ruth

    2009-07-01

    Full Text Available Abstract Background The female lower genital tract is a gateway for pathogens entering the host through the mucous membrane. One of the prevalent human viruses is Torque teno virus (TTV. The major reported routes of TTV transmission are fecal-oral and parenteral. Furthermore, other modes of transmission, e.g. sexual contact, are suggested. To investigate the sexual route of TTV transmission, cervical smears of healthy women and those with cervical lesions were screened for the presence of TTV DNA. Methods TTV DNA was studied in cervical smears of 95 patients with cervical lesions and 55 healthy women. Paired serum samples were available from 55 and 42 women, respectively. All healthy women had normal cytology while 44 patients had histologically confirmed low-grade lesion (LGL and 51 high-grade lesion (HGL. TTV DNA was detected with primers specific for the non-coding region. In 40 paired cervical smears and serum samples, the phylogenetic group of TTV isolates was determined. The presence of HPV DNA in cervical smears was detected by means of PCR with MY09/11 primers. Results The prevalence of TTV DNA in cervical smears of healthy women was 52.7% and was comparable with that in paired serum samples (50%. Symptomatic women had significantly higher prevalence of TTV DNA in cervical smears (74.7% than healthy controls. The TTV DNA prevalence in patient serum samples was 51%. The phylogenetic groups of TTV serum isolates were concordant with those of TTV from cervical smears of the same subjects. In cervical smears, a wider variety of TTV isolates was found. The viral loads in cervical smears were 10 to 1000 times as high as in sera. The HPV-positive study subjects had significantly higher TTV DNA prevalence than HPV negatives. The prevalence of TTV was not associated with disease severity. Conclusion High prevalence of TTV in cervical smears suggests that sexual transmission is another mode of expansion of TTV infection among the population. The

  12. Sialyl Lewis x expression in cervical scrapes of premalignant lesions

    Indian Academy of Sciences (India)

    Noé Velázquez-Márquez; Gerardo Santos López; Lucio Jiménez Aranda; Julio Reyes Leyva; Verónica Vallejo Ruiz

    2012-12-01

    Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumour progression and metastases. Altered expression of glycosidic antigens has been reported in cervical cancer. In cervix premalignant lesions, an increased expression of sialic acid has been reported. In the present study we determined the expression profiles of the glycosidic antigens Tn, sialyl Tn (sTn), Lewis a (Lea), sialyl Lewis a (sLea), Lewis x (Lex) and sialyl Lewis x (sLex) in cervical scrapes with cytological diagnoses of normal, low-grade squamous intraepithelial lesions (LGSIL) and high-grade squamous intraepithelial lesions (HGSIL). Cervical scrapings were collected to detect tumour antigens expressions by flow cytometry using monoclonal antibodies. Cytometry analysis of Tn, sTn, Lea and Lex did not reveal differences at the expression level among groups. The number of positive cells to sLea antigen increased in the HGSIL group with respect to the normal group (=0.0495). The number of positive cells to sLex antigen in the samples increased with respect to the grade of squamous intraepithelial lesion (SIL) ( < 0.001, Mann–Whitney U test). The intensity of expression of this antigen increased in the HGSIL samples with respect to normal samples ( < 0.0068). sLex antigen could be a candidate to be used as biomarker for the early diagnosis of cervical cancer.

  13. Using Intervention Mapping as a Participatory Strategy: Development of a Cervical Cancer Screening Intervention for Hispanic Women

    Science.gov (United States)

    Byrd, Theresa L.; Wilson, Katherine M.; Smith, Judith Lee; Heckert, Andrea; Orians, Carlyn E.; Vernon, Sally W.; Fernandez-Esquer, Maria E.; Fernandez, Maria E.

    2012-01-01

    Cervical cancer is preventable with treatment of precancerous lesions and treatable at early stages. Hispanics have higher rates of cervical cancer and lower rates of screening. "Ayndando a las Mujeres con Informaccion, Guia, y Amor para su Salud" (AMIGAS) is an intervention to increase cervical cancer screening in U.S. women of Mexican origin.…

  14. 中医药治疗胃癌前病变的实验研究进展%Experimental Research on Chinese Medicine Treatment of Gastric Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    谢晶日; 孙芳; 梁国英

    2013-01-01

    The text is to discuss the role of the experimental research on gastric precancerous lesions by the TCM point of view in recent years. This paper reviews the mechanism of the TCM intervention in gastric precancerous lesions which is related to the following points:protecting gastric mucosa,inhibiting and kill Helicobacter pylori,regulating the immune regulation, regulating cell proliferation and apoptosis gene expression of oncogenes and tumor suppressor genes,inhibiting telomerase activity and other aspects of treatment of gastric disease.%从中医药的角度讨论了近年来大鼠实验研究对胃癌前病的作用,认为中医药干预胃癌前病变的机理与以下几点有关:中药可保护胃黏膜、抑杀幽门螺杆菌、调节免疫的作用、调节细胞增殖和凋亡基因的表达及对癌基因和抑癌基因的影响抑制端粒酶活性等环节治疗胃癌前病.

  15. An experimental research of Weining granule in treating gastric precancerous lesions%胃宁颗粒治疗胃癌前病变实验研究

    Institute of Scientific and Technical Information of China (English)

    Xing Deng; Jian Liang; Donghua Liu; Chaoyang Zhu; Longhua Li; Likui Qin

    2009-01-01

    Objective: To investigate potential therapeutic effects and mechanism of Weining granule in the treatment of gastric precancerous lesions. Methods: Sixty rats were randomly assigned to a blank group or a model group or to receive retinoic acid or high-, medium-or low-dose of Weining granule. General conditions of the animals were observed before and after treatment. Changes in gastric mucosal pathohistology, telomerase activity, proliferation index (PI) and apoptosis index (AI) were measured. Results: General conditions, including activity and eating, were improved in all Weining-granule-treated groups with the numbers of rats having intestinal metaplasia (IM), atypical hyperplasia (ATP) or positive telomerase activity being significantly lower than those in the model group (P < 0.05 or P < 0.01). Compared with the model group, all doses of Weining granule significantly decreased PI (P < 0.01) and increased AI (P < 0.05). Conclusion: Weining granule may provide a therapeutic benefit for the treatment of gastric precancerous lesions by inhibiting telomerase activity and proliferation of gastric cancer cells and by accelerating their apoptosis.

  16. ASSESSING PROGRESSION OF CERVICAL PRE-CANCER LESIONS

    OpenAIRE

    Dragos Crauciuc; Ovidiu Toma; Eduard Crauciuc

    2016-01-01

    The purpose of this study was to accomplish a comparative assessment between the immune histochemical and the immunocytochemical expression of p16 protein and L1 major capsid protein of HPV respectively, in cervical squamous intraepithelial lesions with low and high grade, in order to determine, through morphological and clinical correlations, their applicability into practice when diagnosing and further monitoring the patients. There were 119 patients included in the study, havin...

  17. Co-expression of metalloproteinases 11 and 12 in cervical scrapes cells from cervical precursor lesions.

    Science.gov (United States)

    Valdivia, Alejandra; Peralta, Raúl; Matute-González, Manuel; García Cebada, Juan Manuel; Casasola, Ivonne; Jiménez-Medrano, Cristina; Aguado-Pérez, Rogelio; Villegas, Vanessa; González-Bonilla, Cesar; Manuel-Apolinar, Leticia; Ibáñez, Miguel; Salcedo, Mauricio

    2011-01-01

    The metalloproteinases (MMP) 11 and 12 have been shown to be expressed in cervical cancer (CC). In order to extend our previous results, these MMPs were evaluated in cervical precursor lesions. One hundred seventeen cervical scrapes: thirty-six normal, thirty-six low grade squamous lesions (LSIL), thirty-six high grade (HSIL), nine CC; and, also ninety-nine paraffin-embedded cervical lesions: fifteen normal cervices, thirty eight LSIL, sixteen HSIL, and five CC were collected. The samples were analyzed for relative expression by real time RT-PCR or immunohistochemistry assay. We were able to identify a relative increased expression of MMP11 in 75% and 78% from LSIL and HSIL samples, respectively. While MMP12 expression was 64% and 75% in LSIL and HSIL, respectively. Positive samples for MMP11 expression were also positive for MMP12 expression and also increased according to illness progression. In the tissues, MMP11 or MMP12 expression was observed in the cytoplasm of the neoplastic cells, while in the normal epithelium was absent. The reaction was always stronger for MMP12 than MMP11. MMP11 expression was present in 77% and 66% of LSIL and HSIL, while MMP12 expression was 73% and 68%. There was a relationship between MMP11 or MMP12 expression and HPV infection. Our data are showing a relationship between diagnostic of precursor lesions and the MMP11 and 12 expressions, suggesting that their expression could be an early event in the neoplastic lesions of the cervix and could have clinical significance.

  18. 大鼠胃癌前病变模型的建立%Development of gastric precancerous lesion animal model

    Institute of Scientific and Technical Information of China (English)

    李春英; 郭静; 梁爱华; 高双荣; 回连强; 刘婷; 曹春雨; 赵雍; 郝然; 易艳

    2012-01-01

    目的:采用含马兜铃酸中药关术通建立一种大鼠胃黏膜癌前病变模型.方法:将SD大鼠按体重随机分为对照组和关木通乙醇提取物不同剂量组(以马兜铃酸Ⅰ计分别为2.5,5,10mg·kg1).各组隔目灌胃给予1次.分别于给药后第10,15,20周,分批从每组抽取部分动物处死,解剖,称量胃质量,折算胃指数和胃肿瘤质量.将胃固定于4%巾性甲醛溶液中,常规进行石蜡包埋、切片、HE染色,光学显微镜下观察胃组织形态学改变,记录各组发牛胃黏膜良性增生、胃黏膜癌前病变(Ⅰ,Ⅱ,Ⅲ度不典型增生)和恶性胃癌发生的动物数.结果:大鼠灌胃给予关木通提取物(分别相当于马兜铃酸Ⅰ2.5,5.0,10.0mg·kg1-1)10周时,各剂量组可见前胃黏膜乳头状瘤增生.随造模时间延长,乳头状瘤数量增多.相当于马兜铃酸Ⅰ5.0,10.0mg·kg-1剂量组造模10周时,即可见胃癌前病变,阳性率较高.造模15周时,相当于马兜铃酸Ⅰ5.0mg·kg-1及其以上剂量组的胃癌前病变发生率高达100%.造模20周时,可见多数动物发生胃癌.结合动物耐受情况、胃黏膜癌前病变发生率以及肿瘤发展情况,认为相当于马兜铃酸Ⅰ5.0mg·kg-1的剂量为最适宜造模剂量,癌前病变阶段为造模10~15周.结论:大鼠灌胃给予含马兜铃酸中药关木通提取物可以在较短时内形成理想的胃癌前病变模型.%Objective: To establish a model of gastric precancerous lesion by using Aristolochic manshuriensis which contains aristoloehic aoids. Method: The SD rats were randomly divided into four groups: control and three different doses of ethanol extractive of/4, manshuriensis ( EEA) (corresponding to aristoloehic acid I 2.5,5.0, 10.0 mg·kg-1), respectively. EEA was intragastrical-ly given to rats every other day. At the end of the 10th, 15th , 20th week, part of the rats in each group was sacrificed and the stomachs were weighed. The gastric tumor was assessed by the

  19. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, Mandy; Scholtka, Bettina, E-mail: scholtka@uni-potsdam.de [Chair of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, Arthur- Scheunert-Allee 114-116, 14558 Nuthetal (Germany); Gottschalk, Uwe [Maria Heimsuchung Caritas-Klinik Pankow, Breite Straße 46/47, 13187 Berlin (Germany); Faiss, Siegbert [III. Medizinische Abteilung - Gastroenterologie und Hepatologie, Asklepios Klinik Barmbek, Rubenkamp 220, 22291 Hamburg (Germany); Schatz, Daniela; Berghof-Jäger, Kornelia [BIOTECON Diagnostics GmbH, Hermannswerder Haus 17, 14473 Potsdam (Germany); Steinberg, Pablo, E-mail: scholtka@uni-potsdam.de [Chair of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, Arthur- Scheunert-Allee 114-116, 14558 Nuthetal (Germany); Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover (Germany)

    2010-12-29

    In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567) by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study) and neoplastic lesions (in a previous study) of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer.

  20. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

    Directory of Open Access Journals (Sweden)

    Daniela Schatz

    2010-12-01

    Full Text Available In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567 by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study and neoplastic lesions (in a previous study of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer.

  1. Detection of up to 65% of Precancerous Lesions of the Human Colon and Rectum by Mutation Analysis of APC, K-Ras, B-Raf and CTNNB1

    International Nuclear Information System (INIS)

    In the present study a recently conceived 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signaling pathways was used to analyze 20 colorectal serrated lesions and 41 colorectal adenoma samples and to determine the percentage of each of the above-mentioned potentially precancerous lesions carrying at least one of the four above-mentioned genes in a mutated form. CTNNB1 and B-Raf were screened by PCR-single-strand conformation polymorphism analysis, K-Ras by restriction fragment length polymorphism analysis and the APC gene mutation cluster region (codons 1243–1567) by direct DNA sequencing. APC mutations were only detected in 10% of the serrated lesions but in 34% of the adenomas. Twenty percent of the serrated lesions and 14% of the adenomas carried a mutated K-Ras. B-Raf was found to be mutated in 50% of the serrated lesions and in 22% of the adenomas. CTNNB1 was altered in 12% of the adenomas, but not in serrated lesions. By using the above gene marker panel it could be shown that 65% of the serrated lesions and 61% of the adenomas carried at least one of the four genes in a mutated form. Based on its excellent performance in detecting mutations in sporadic preneoplastic (in this study) and neoplastic lesions (in a previous study) of the human colon and rectum, this primer combination might also be suited to efficiently and non-invasively detect genetic alterations in stool DNA of patients with early colorectal cancer

  2. N-nitroso compounds, genotoxins and their precursors in gastric juice from humans with and without precancerous lesions of the stomach.

    Science.gov (United States)

    Pignatelli, B; Malaveille, C; Chen, C; Hautefeuille, A; Thuillier, P; Muñoz, N; Moulinier, B; Berger, F; De Montclos, H; Ohshima, H

    1991-01-01

    We are investigating the interrelationships between levels of total N-nitroso compounds (NOC), genotoxic activity (both before and after nitrosation), degree of bacterial colonization in gastric juice and degree of severity or absence of precancerous lesions of the stomach. The mean level of constitutive total NOC in gastric juice was similar in the different groups of patients, but it was higher in acidic gastric juice (n = 30) than in gastric juice at pH greater than 4.5 (n = 12). Acid-catalysed nitrosation of gastric juice in vitro increased the concentration of total NOC by up to several thousand fold, to a maximum of 1330 mumol/l. Genotoxicity, expressed as SOS-inducing potency per 100 microliters of gastric juice was measurable in only 20% of gastric juice samples tested. After acid-catalysed nitrosation, however, all samples showed genotoxic activity, the mean SOS-inducing potency being four to seven times greater than the corresponding constitutive value. There was no association between the mean SOS-inducing potency of gastric juice and the severity of precancerous lesions. The mean SOS-inducing potency of neutral or basic gastric juice was slightly greater than that of acidic samples. In a kinetic study on N-nitrosation of gastric juice in vitro, a mixture of amino and amido substrates was nitrosated; both qualitative and quantitative individual differences in nitrosatable substrates in gastric juice were seen. Fractionation of acidic, neutral and basic nitrosated gastric juice samples revealed a preponderance of nonvolatile, unknown NOC with varying polarities. The results of our study suggest that only pH determines the nature and level of precursors of NOC and of nitrosation-dependent genotoxins in gastric juice. PMID:1855844

  3. Implications of tyrosine phosphoproteomics in cervical carcinogenesis

    Directory of Open Access Journals (Sweden)

    DeFord James

    2008-01-01

    Full Text Available Abstract Background Worldwide cervical cancer remains a leading cause of mortality from gynecologic malignancies. The link between cervical cancer and persistent infection with HPV has been established. At a molecular level little is known about the transition from the precancerous state to invasive cancer. To elucidate this process, cervical biopsies from human specimens were obtained from precancerous state to stage III disease. Methods Cervical biopsies were obtained from patients with a diagnosis of cervical cancer undergoing definitive surgery or staging operation. Biopsies were obtained from patients with precancerous lesions at the time of their excisional procedure. Control samples were obtained from patients undergoing hysterectomy for benign conditions such as fibroids. Samples were subjected to proteomic profiling using two dimensional gel electrophoresis with subsequent trypsin digestion followed by MALDI-TOF protein identification. Candidate proteins were then further studied using western blotting, immunoprecipitation and immunohistochemistry. Results Annexin A1 and DNA-PKcs were found to be differentially expressed. Phosphorylated annexin A1 was up regulated in diseased states in comparison to control and its level was strongly detected in the serum of cervical cancer patients compared to controls. DNA-PKcs was noted to be hyperphosphorylated and fragmented in cancer when compared to controls. By immunohistochemistry annexin A1 was noted in the vascular environment in cancer and certain precancerous samples. Conclusion This study suggests a probable role for protein tyrosine phosphorylation in cervical carcinogenesis. Annexin A1 and DNA-PK cs may have synergistic effects with HPV infection. Precancerous lesions that may progress to cervical cancer may be differentiated from lesions that will not base on similar immunohistochemical profile to invasive squamous cell carcinoma.

  4. REPRODUCTIVE ASPECTS ASSOCIATED WITH PRECURSOR LESIONS FOR CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Rogério Ferrari

    2013-05-01

    Full Text Available To investigate the relationship between reproductive variables and theprecursor lesions for cervical cancer in women attended the clinic of the lower genitaltract pathology and colposcopy (PTGIC, packed in the complex regional healthCaceres city, southwest of Mato Grosso, in the year 2009.Methods:We conducted across sectional study with data collection from medical records of 142 women withabnormal cytology, colposcopy and positive underwent directed biopsy, taking intoaccount the reproductive variables.Results:indicate that the minimum age at menarchewas 9 years and maximum age was 17 years, mean 12.6 years; on the average paritywas 3.4 children, the use of hormonal contraceptive method corresponds to 34 5% and38.1% female sterilization research, 46.5% are or have use of contraception for a periodgreater than five years, with a mean of 4.7 years and 67.2% of women surveyed do notuse condoms. The variables were not significantly associated to cervical cancer.Conclusion:the characteristics of the women studied may serve as a basis for workdirected to this population in order to seek to minimize this problem.Although the dataobtained were satisfactory, it was possible to trace the profile of the reproductiveaspects of women in the Clinic ofPTGIC, there is a lack of association betweenreproductive aspects and findings of the cervical biopsy, it may be associated with somelimiting factors of the study that need to be balanced

  5. Cervical Lesions in Women Younger than 25 Years

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a diciembre de 2007 y primer trimestre del 2008. Se analizaron: edad, edad de primera relación sexual, número de parejas sexuales, tipo de lesión, grado de la lesión y motivo de consulta. Resultados: predominaron las mujeres de más de 21 años y las que tuvieron 2 y 3 parejas sexuales; el principal motivo de consulta fue la cervicitis (84, 7 %; en el 22,6 % de las mujeres se detectó neoplasia intraepitelial grado I, en el 41, 9 % de grado II y en el 33, 1 % de grado III. En el 90 % de las pacientes más jóvenes la lesión fue  maligna. Conclusiones: la presencia de lesiones de cuello uterino en mujeres menores de 25 años no es baja, como tampoco la frecuencia de lesiones malignas.

  6. TCT联合HPV检测在宫颈病变筛查中的意义%Cervical Liquid-Based Cytology Combined Human Papillomavirus Testing in Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    戴一菲; 田荣华

    2012-01-01

    Objective To study the significance of combination of cervical liquid-based cytology and human papillomavirus detection in cervical lesion screening. Methods From March 2010 to December 2011,3,028 patients were detected with nipple shaped tumor virus ( HPV) and cervical liquid base cytology (TCT) ,TCT was used by 2001 International Cancer Association TBS diagnosis system report way,to the patients with positive results of TCT check for ASCIIS and the above lesions and (or) HPV detection, cervical organization biopsy under electronic vaginal mirror were performed. Results The sensitivity of TCT check was 56.72% ,with the specificity of 28. 19% ;the sensitivity of HPV check was 86.03% ,with specific for 46.38% ;among 408 cases of CIN and cervical cancer pathology ,28 cases were single TCT positive (6. 86% ) ;with TCT and HPV positive 203 ca-ses(49. 75% ) ,there was significant difference in diagnosis of cervical squamous intraepithelial neoplasm ( CIN) and cervical cancer(x2 = 184.926,P=0.000) between single TCT check and combination of TCT and HPV examination. Conclusion TCT combined with HPV check can improve the detection rate of cervical cancer and precancerous lesions, improve the sensitivity and specificity of screening,effectively reduce the TCT in detection of false-negative rates,and avoid excessive colposcopy and biopsy in low-risk populations and over-treatment.%目的 探讨宫颈液基细胞学检查与人乳头状瘤病毒检测联合应用在宫颈病变筛查中的意义.方法 选取2010年3月-2011年12月孝感市中心医院病理科同时进行了宫颈液基细胞学(TCT)检查及人乳头状瘤病毒(HPV)检测的患者3028人,TCT采用2001年国际癌症协会TBS诊断系统报告方式,对TCT检查结果为ASCUS及以上病变和(或)HPV检测结果为阳性的患者在电子阴道镜下取宫颈组织活检.结果 TCT检查敏感度为56.72%,特异度为28.19%;HPV检查敏感度为86.03%,特异度为46.38%;病

  7. Screening Methods for Cervical Lesion and its New Development%宫颈病变筛查方法及其新进展综述

    Institute of Scientific and Technical Information of China (English)

    邓晓红

    2016-01-01

    综述宫颈病变的筛查技术及新进展,早期发现宫颈癌和提高治愈率。宫颈癌是最常见的妇科恶性肿瘤之一,严重威胁着妇女的健康,且近几年趋向年轻化。人乳头瘤病毒(HPV DNA)感染是引起宫颈病变尤其是宫颈癌的主要原因,TCT是宫颈细胞学筛查的一种新的全自动细胞学检测技术,阴道镜是宫颈早期浸润癌筛查的重要应用。三者联合筛查宫颈病变,早期发现、早期诊断和早期干预宫颈的癌前病变,能够有效预防宫颈癌发生,降低宫颈癌的发病率和死亡率。%This paper reviews screening technologies and new developments for cervical lesions, early detection of cervical cancer, so as to improve in cure rate. Cervical cancer is one of the most common gynecologic malignant tumor, it is a serious threat to women's health, and in recent years it trends to target the younger ages. Human papilloma virus (HPV DNA) infection is the primary cause of cervical lesions, especially for cervical cancer. TCT is a new automatic cytology detection technology for cervical cytology screening. Colposcopy is an important application in early invasive cervical cancer screening. Combining the above mentioned three screening technologies could achieve the purposes of early detection, early diagnosis and early intervention of cervical precancerous lesions, so as to effectively prevent the occurrence of cervical cancer, reduce the morbidity and mortality of cervical cancer.

  8. Expression of Inducible Nitric Oxide Synthase, p53 and Bcl-2 in Gastric Precancerous and Cancerous Lesions: Correlation with Clinical Features

    Institute of Scientific and Technical Information of China (English)

    Tao Cui; Zu'an Zhu; Ying Liu; Qingyan Kong; Sujuan Fei

    2006-01-01

    OBJECTIVE To explore the expression of inducible nitric oxide synthase(iNOS), p53 and bcl-2 in gastric precancerous and cancerous lesions and to examine the expression of these proteins in relation to clinical features.METHODS The expressions of iNOS, p53 and bcl-2 proteins in gastric precancerous and cancerous lesions and their correlations with the clinical features were determined using immunohistochemical assays (Power VisionTM two-step method) on 84 gastric carcinomas and 54 gastric atypical hyperplastic tissues. Apoptotic cells were evaluated by terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick-end labeling (TUNEL).RESULTS Expression of iNOS, p53 and bcl-2 was significantly higher in gastric carcinoma (GC) tissues than in gastric atypical hyperplastic tissues. Among the 84 carcinomas, the expression of p53 was observed in 50 (59.52%), bcl-2 in 43 (51.19%), and iNOS in 65 (77.58%). Overexpression of iNOS and bcl-2 in gastrlc carcinoma was related to tumor size and iNOS was related to the presence of lymph node metastasis (P<0.05). The expression of proteins did not correlate with age, sex, stage of disease, or differentiation. Expression of iNOS in gastric carcinoma tissues was positively correlated with bcl-2 expression (χ2=8.926, P=0.003),and also with p53 expression (χ2= 5.2430, P= 0.022). The mean apoptotic indexes (Al) were 1.29%±0.50 in low-grade atypical hyperplasia (LG),0.96%±0.36 in high-grade atypical hyperplasia (HG) and 0.70%±0.43 in GC, with the difference being significant between LG, HG and GC (P<0.05). There was a significant positive correlation between iNOS expression and the Al in GC (t=3.0815, P=0.0028).CONCLUSION iNOS was expressed in the majority of gastric carcinoma tissues and correlated with cellular apoptosis associated with p53 and bcl-2 expression. iNOS overexpression is closely associated with p53 and bcl-2 accumulation status. iNOS may play a synergistic role in the pathogenesis of GC.

  9. Chromosome 11 aneusomy in esophageal cancers and precancerous lesions-an early event in neoplastic transformation: An interphase fluorescence in situ hybridization study from south India

    Institute of Scientific and Technical Information of China (English)

    Vasavi Mohan; Shivani Ponnala; Hemakumar M Reddy; Radha Sistla; Rachel A Jesudasan; Yog Raj Ahuja; Qurratulain Hasan

    2007-01-01

    AIM: To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies.METHODS: We performed fluorescence in situ hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control)obtained from patients referred to the clinic for endoscopic evaluation.RESULTS: Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers.CONCLUSION: Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers.

  10. COMPARATIVE STUDY OF EVALUATION OF SENSITIVITY AND SPECIFICITY OF CYTOLOGY AND COLPOSCOPY FOR DETECTION OF PRECANCEROUS LESIONS OF CERVIX

    Directory of Open Access Journals (Sweden)

    Sharma

    2013-12-01

    Full Text Available ABSTRACT : BACKGROUND : Cervical cancer is the second most common cancer among women globally and a leading cause of death due to cancer among women. If diagnosed earlier, it has a high cure rate but advanced disease is frequently incurable with v ery unpleasant consequences. MATERIAL AND METHODS : This was a cross sectional study done on a group of 50 women. A detailed clinical history of each patient was taken. The patients were made to lie in dorsal lithotomy position and per speculum examination was done. After evaluating the cervix, Paps smear was taken for cytology with Ayers spatula from ectocervix after rotating at 360 degree and spread on 2 glass slides to prepare thin films and kept in koplin jar and sent for cytology reporting. The patients were then subjected to colposcopy and punch biopsy. RESULTS : On comparison with biopsy cervix as gold standard for detection of CIN, cytology had shown Sensitivity - 57.14%, Specificity - 88.88%, NPV - 84.21%, PPV - 66.66%, whereas colposcopy had shown Sen sitivity - 80%, Specificity - 80%, NPV - 94.11%, PPV - 50%. CONCLUSION : In developing countries like India, cytology, a low cost and easily accessible test, is the most logical screening modality although it has very low sensitivity but detection rates coul d be further improved using liquid cytology and use of endocervicalcytobrush. Colposcopy with high sensitivity is although an important adjunctive screening modality but requires expertise and training for interpretation and is costly

  11. Human telomerase RNA component (hTERC gene amplification detected by FISH in precancerous lesions and carcinoma of the larynx

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    Liu Yu

    2012-03-01

    Full Text Available Abstract Background Gain of 3q26 is frequently observed in squamous cell carcinomas of mucosal origin, including those originating in the head and neck region. The human telomerase RNA component (hTERC gene, which is located on chromosome 3q26, encodes for an RNA subunit of telomerase that maintains the length of telomeres through cellular divisions, and is activated in malignant diseases. The present study was designed to detect hTERC amplification in laryngeal lesions and evaluate whether this might serve as a supportive biomarker in histopathological analysis for in the diagnosis of laryngeal lesions. Methods Fluorescent in situ hybridization (FISH was applied on formalin-fixed paraffin-embedded blocks of 93 laryngeal specimens, including 14 normal epithelium (NE, 15 mild dysplasia (Md, 18 moderate dysplasia (MD, 16 severe dysplasia (SD, 9 carcinoma in situ (CIS, and 21 invasive carcinoma (IC. Results By histopathologic examination, hTERC amplification rates in NE, Md, MD, SD, CIS and IC cases were 0% (0/14, 13.33% (2/15, 72.22% (13/18, 81.25% (13/16, 100% (9/9 and 100% (21/21, respectively. Amplification of hTERC was significantly associated with histopathologic diagnosis (P Conclusions The hTERC amplification is important in the development of laryngeal squamous cell carcinoma (LSCC. FISH detection of hTERC amplification may provide an effective approach in conjunction with histopathologic evaluation for differential diagnosis of laryngeal lesions. Virtual Slides The virtual slide(s for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2226606266791985

  12. Cervical Pap smear study and detection of abnormal epithelial lesions and determination of its accuracy by cytohistological correlation in patients of tertiary care teaching hospital in central India

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    Garima Malpani

    2016-07-01

    Conclusions: Pap smear is a cost effective and sensitive screening method for detection of cancerous, pre-cancerous and non-cancerous lesions of cervix. [Int J Reprod Contracept Obstet Gynecol 2016; 5(7.000: 2312-2316

  13. Mutant KRas-Induced Mitochondrial Oxidative Stress in Acinar Cells Upregulates EGFR Signaling to Drive Formation of Pancreatic Precancerous Lesions

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    Geou-Yarh Liou

    2016-03-01

    Full Text Available The development of pancreatic cancer requires the acquisition of oncogenic KRas mutations and upregulation of growth factor signaling, but the relationship between these is not well established. Here, we show that mutant KRas alters mitochondrial metabolism in pancreatic acinar cells, resulting in increased generation of mitochondrial reactive oxygen species (mROS. Mitochondrial ROS then drives the dedifferentiation of acinar cells to a duct-like progenitor phenotype and progression to PanIN. This is mediated via the ROS-receptive kinase protein kinase D1 and the transcription factors NF-κB1 and NF-κB2, which upregulate expression of the epidermal growth factor, its ligands, and their sheddase ADAM17. In vivo, interception of KRas-mediated generation of mROS reduced the formation of pre-neoplastic lesions. Hence, our data provide insight into how oncogenic KRas interacts with growth factor signaling to induce the formation of pancreatic cancer.

  14. Detection of the tumor markers in patients with gastric precancerous lesions%胃癌癌前病变相关标志物的检测

    Institute of Scientific and Technical Information of China (English)

    刘中娟; 程歆琦; 郭子建; 赵丽娜; 邱玲

    2013-01-01

    It is confirmed that chronic atrophic gastritis (CAG) caused by Helicobacter pylori is the main cause of gastric precancerous lesions.CAG is also the key determinant in gastric cancer risk assessment,which affects pepsinogen and gastrin-17 secretion.Most of the gastric cancer patients have poor prognosis,and non-invasive tools for gastric cancer screening and diagnosis are lacking.Therefore,the early detection of gastric cancer in order to reduce the disease mortality is necessary.Pepsinogen and gastrin-17 are biomarkers of gastric mucosa and gastric antra.The serological testing for the stomach-specific biomarkers offers the possibility to know preneoplastic gastric mucosal conditions.%研究证实,幽门螺旋杆菌感染引起的慢性萎缩性胃炎是诱发胃癌的主要癌前病变,是胃癌风险评估的关键并影响胃蛋白酶原(PG)和胃泌素-17(G-17)分泌.大多数胃癌患者预后差,且缺乏非侵袭性的筛查和诊断手段,因此早期发现胃癌以降低胃癌病死率非常必要.PG和G-17分别为胃体黏膜和胃窦的生物标志物,这些特异性生物标志物的检测为了解肿瘤发生前胃黏膜的状况提供了可能.

  15. A Correlative Analysis of Cervical Lesions in Patients with Vulva Condyloma Acuminatum

    Institute of Scientific and Technical Information of China (English)

    Qing Li; Weihong Li; Huanzi Li; Zhihua Liu

    2006-01-01

    OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions.METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, highrisk HPV-DNA test and cervical bioscopy.RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV (+) infections were detected in 68.7% (287/418) of the cases. Among those patients, 20.6% (59/287) had concurrent subclinical cervical intraepithelial neoplastic (CIN) lesions. Among the high-risk HPV(-) 31.3 % (131), patients 7.6% (10/131) had concurrent subclinical CIN lesions. Pathological examination results: cervicitis,167 (40.0%); cervical HPV infection, 182 (43.5%); CIN-Ⅰ, 51(12.2%); CIN-Ⅱ, 16 (3.83%); CIN-Ⅲ, 2 (0.5%); cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia (VIN) Ⅱ~Ⅲ.CONCLUSION It was concluded that simultaneous cervical HPV infection of many types was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.

  16. The clinical significance of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers%液基细胞学用于外来工妇女宫颈癌前病变筛查的临床意义

    Institute of Scientific and Technical Information of China (English)

    卢晓艳; 谢家滨; 杨步琴

    2011-01-01

    目的:探讨液基细胞学检查用于外来工妇女宫颈癌前病变筛查中的诊断价值及宫颈癌筛查现状.方法:应用液基细胞学(TCT)对到我院门诊就诊的外来工妇女(外来组)2 500名和进行常规宫颈癌前病变筛查的常住妇女1 600名(本地组)进行对比,并对其中细胞学筛查异常者进行阴道镜及镜下宫颈活检病理检查.结果:外来工妇女中,检测前有阴道排液或性生活出血等症状者878例,占35.12%,本地组有症状者87例,占5.43%.外来组共检测出细胞阳性368例,占14.72%,本地组异常例数为110例,占6.87%,两组比较,差异有统计学意义(P<0.05).对细胞学检查异常者进行阴道镜及镜下宫颈活检病理检查,其中CINⅠ级以上360例,符合率达75.3%.结论:门诊就诊的外来妇女与本地妇女相比较,宫颈癌前病变发病率较高,有症状者来院就诊比率明显升高,外来组妇女对宫颈癌前病变筛查认识普及率低下,因此常规对外来工就诊妇女进行液基细胞学检查,并结合阴道镜宫颈活检,是筛查和诊断宫颈癌前病变的可靠手段.%Objective: To discuss the diagnostic value of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers and to survey the status of screening tests for precancerous lesions of the uterine cervix. Methods: 2 500 female migrant workers who went to our hospital for treatment in the method of liquid based cytology (TCT) compared with 1 600 towns women who went to our hospital for routine examination in screening tests for precancerous lesions of the uterine cervix, then examined the women whose index were abnormal by means of colposcopy and cervical biopsy. Results: 878 female migrant workers had the symptoms of draining the liquid from vagina before examination and bleeding during intercourse, accounting for 35.12%; and 87 towns women had the symptoms, accounting for 5.43%. There were 368 cases

  17. Glass ionomer cements and their role in the restoration of non-carious cervical lesions

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    Luciana Fávaro Francisconi

    2009-10-01

    Full Text Available Glass ionomer based materials are clinically popular in several areas of restorative dentistry, but restoration of cervical lesions has proven particularly successful. Various etiologies, conformations, locations and structural characteristics make non-carious cervical lesions more challenging to adhesive restorative procedures and marginal seal in the long run. Due to their characteristics, glass ionomer cements (GICs have precise indication for these cases. Moreover, the use of a GIC base underneath composite resin, the so-called "sandwich" or mixed technique, allows associating the good characteristics of composite resins and GICs, and has been considered quite useful in the restoration of non-carious cervical defects. The aim of this paper is to critically review the literature and discuss peculiar features of GICs regarding their role in the restoration of non-carious cervical lesions.

  18. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women

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    Vidal Adriana C

    2011-11-01

    Full Text Available Abstract Background Infection with human papillomavirus (HPV is associated with uterine cervical intraepithelial neoplasia (CIN and invasive cancers (ICC. Approximately 80% of ICC cases are diagnosed in under-developed countries. Vaccine development relies on knowledge of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes remain poorly characterized in many African countries. To contribute to the characterization of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies were obtained for cytology and HPV DNA detection. Results 79 out of 215 (36.7% enrolled participants tested positive for HPV DNA, with a large proportion being multiple infections (74%. The prevalence of HPV infection increased with lesion grade (14% in controls, 67% in CIN1 cases and 88% in CIN2-3. Among ICC cases, 89% had detectable HPV. Overall, 31 HPV genotypes were detected; the three most common HPV genotypes among ICC were HPV16, 35 and 45. In addition to these genotypes, co-infection with HPV18, 31, 33, 52, 58, 68 and 82 was found in 91% of ICC. Among women with CIN2-3, HPV53, 58 and 84/83 were the most common. HPV35, 45, 53/58/59 were the most common among CIN1 cases. Conclusions In women with no evidence of cytological abnormalities, the most prevalent genotypes were HPV58 with HPV16, 35, 52, 66 and 73 occurring equally. Although numerical constraints limit inference, findings that 91% of ICC harbor only a small number of HPV genotypes suggests that prevention efforts including vaccine development or adjuvant screening should focus on these genotypes.

  19. Expression of gastric cancer-associated MG7 antigen in gastric cancer, precancerous lesions and H. pylori-associated gastric diseases

    Institute of Scientific and Technical Information of China (English)

    Dong-Li Guo; Ming Dong; Lan Wang; Li-Ping Sun; Yuan Yuan

    2002-01-01

    AIM: To investigate the relationship between the antigen MG7 antigen expression and gastric cancer as well as precancerous condition; to study the relationship between the MG7 antigen expression and H. pyloriinfection in benign gastric lesions in order to find out the effect of H. pylori infection on the process of gastric cancer development.METHODS: The level of MG7 antigen expression was determined by immunohistochemical method in 383 gastric biopsied materials. The intestinal metaplasia was determined by histochemistry method. The H. pyloriinfection was determined by HE stain, PCR and ELISA in 291 specimens, among which only 34 cases of H. pylori-associated gastric lesions were followed up.RESULTS: The positive rate of MG7 expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric cancer increased gradually in ascending order (P<0.01). The positive rate of MG7 antigen expression in type Ⅲ intestinal metaplasia of gastric mucosa was higher than that of type Ⅰand Ⅱ intestinal metaplasia, being highly significant (P<0.05).The positive rate of MG7 antigen expression in superficial gastritis, atrophic gastritis and gastric cancer increased gradually (11.9 %, 64.8 %, 91.2 %, P<0.01). There was no significant difference between H.pylori-negative and H. pyloripositive intestinal metaplasia, atrophic gastritis and dysplasia of gastric epithelium in the positive rate of MG7 antigen expression. There was no expression of MG7 antigen in H. pylori-negative superficial gastritis. The positive rate of MG7 expression in H. pylori-positive superficial gastritis was 20.5 %, and the difference between them was significant (P<0.05). During following up, one of the three H. pylori negative cases turned positive again, and its MG7 antigen expression turned to be stronger correspondingly. 3 of 31 H. pyloripositive cases were detected as early gastric cancer, among which one with "+++" MG7 antigen expression was diminished after H. pylori

  20. APPLICATION OF THE 2014 BETHESDA SYSTEM FOR REPORTING OF CERVICAL/VAGINAL CYTOLOGICAL LESIONS

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    Pratima

    2015-12-01

    Full Text Available Pap smear (Conventional smear is the most widely used cervical cancer screening test in the world. Negative intraepithelial lesion for malignancy, squamous intraepithelial lesion and cervical cancer remain important health problems for women worldwide. In developing countries like India, there is a great need for mass screening program for cervical lesion. AIMS AND OBJECTIVES To assess the clinician utility of The 2014 Bethesda System Reporting of cervical and vaginal epithelial abnormalities. MATERIAL AND METHODS All the cervical/vaginal cytology smears reported were retrieved and reviewed in the duration from 2007, 2008, 2009 and 2014. RESULTS A total number of 1700 cases of Pap smears were retrieved during period of study, out of which 90.0% were satisfactory for evaluation; 35.5% were within normal limit and 55.6% were NILM. A few (8.14% of the cases were reported as squamous intraepithelial lesion; 0.5% as atypical squamous cells and 0.19% were reported as atypical glandular cells. CONCLUSION Pap smear is a proven tested tool for making an early diagnosis and treating cervical cancer in early stage. Thus, Pap smear is simple, less expensive diagnostics tool suitable for implementation in India.

  1. Computed tomography in the foramen magnum and high cervical cord lesion

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    Isu, T.; Ito, T.; Iwasaki, Y.; Tsuru, M. (Hokkaido Univ., Sapporo (Japan). School of Medicine); Kitaoka, K.

    1981-10-01

    The value of computed tomography in the spine and spinal cord disease recently has been well documented. However, little attention is made to the role of computed tomography in foramen magnum and high cervical cord lesion. We report 16 cases of the foramen magnum and high cervical cord lesion. Method: Either EMI Head Scanner, CT 1010 (slice thickness 10 mm) or EMI Whole Body Scanner, CT 5005 (slice thickness 13 mm) was used. In 9 cases enhanced CT with intravenous injection of contrast medium was performed. Eleven cases received intrathecal injection of metrizamide. Materials: 1) control group without cervical pathology 70 cases, 2) spinal cord tumor 6 cases, 3) atlanto-axial dislocation 6 cases, 4) Chiari malformation (type 1) 3 cases, 5) spinal foreign body (acupuncture needle) 1 case. Results: 1. plain CT. 1) In control group without cervical pathology the cervical cord at C/sub 1/ level was seen as area surrounded by ring of subarachnoid space. 2) In 14 cases except for 1 case of atlanto-axial dislocation and 1 case of spinal foreign body identification of the cervical cord at C/sub 1/ level was impossible. 3) CT was of diagnostic value in detecting altanto-axial dislocation and spinal foreign body. 2. enhanded CT. Meningioma and neurinoma showed positive contrast enhancement. However, astrocytoma and herniated cerebellar tonsils were not detectable. 3. CT metrizamide myelography visualized the subarachnoid space and made it possible to localize the lesion in the spinal canal in relation to the spinal cord. Conclusion: Attention should be given to plain CT finding at C/sub 1/ level in diagnosis of foramen magnum and high cervical cord lesion. If identification of cervical cord at C/sub 1/ level is impossible, it is suspected that subarachnoid space is blocked by the lesion, and enhanced CT and CT metrizamide myelography must be performed.

  2. Correlation analysis of high-risk human papillomavirus viral load and cervical lesions

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    Xiao-xing MA

    2012-05-01

    Full Text Available Objective  To explore the association between high-risk human papillomavirus (HR-HPV viral load and pathological grades of cervical intraepithelial neoplasia (CIN and cervical cancer. Methods  A total of 1248 patients from General Hospital of PLA, who underwent colposcopy and surgery due to cervical lesions between Jan. 2006 and Aug. 2011 were enrolled in this study, and they were divided five groups: cervicitis, CIN Ⅰ, CIN Ⅱ-Ⅲ, stage Ⅰ cervical cancer and stage Ⅱ cervical cancer. HR-HPV viral load (RLU/CO was determined by the Hybrid Capture Ⅱ (HCⅡ system, and they were categorized into five groups: 0-0.99, 1.00-9.99, 10.00-99.99, 100.00-999.99, ≥1000.00. The mean value and standard deviation of different HR-HPV viral load in the patients with cervicitis or with CIN Ⅰ, CINⅡ-Ⅲ, stage Ⅰ cervical cancer or stage Ⅱ cervical cancer were compared, and the correlation of HR-HPV viral load and pathogenesis of cervical lesions was analyzed. Results  HPV viral loads were significantly higher in CINⅠ(842.1±983.9, CINⅡ-Ⅲ (690.1±795.0, stage Ⅰ cervical cancer (893.1±974.2 and stage Ⅱ cervical cancer (699.5±908.3 patients than in cervicitis patients (274.2±613.6, P < 0.05, and the HPV viral loads in CINⅠ(842.1±983.9 and stage Ⅰ cervical cancer patients were higher than those in CINⅡ-Ⅲ patients (P < 0.05. When HR-HPV viral load was ≥100RLU/CO, the risk of CIN and cervical cancer increased with the increase in viral load, but there was no correlation between the viral load and pathological grades of cervical lesions. In the patients with stage ⅠB-Ⅱ cervical squamous cell carcinoma, when the HR-HPV viral load was ≥100RLU/CO, the risk of lymph node metastasis increased (P < 0.05, and the number of patients with maximum diameter of the cervical tumor ≥4cm also increased (P < 0.05. However, the HR-HPV viral load was not correlated with patient age, pathological type of the lesion, depth of cancer

  3. Value of methylene blue chromoendoscopy in detection of early gastric cancer and precancerous lesions%亚甲蓝色素内镜对早期胃癌及癌前病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    冯业全; 陈雪; 陈万宁; 杨清清; 刘倩

    2012-01-01

    Objective To investigate the value of methylene blue chromoendoscopy in the detection of early gastric cancer and precancerous lesions. Methods 194 outpatients and inpatients in our hospital from January 2010 to December 2011 were divided into the experimental group and the control group according to different endoscopic examinations. The experimental group(104 cases) was performed the methylene blue chromoendoscopy and the control group(90 cases) received the conventional gastroscopic examination. The detection rates of early gastric cancer and precancerous lesion were compared between the two kinds of endoscopy. Results The detection rate of glandular epithelial intestinal metaplasia, atypical hyperplasia and adenocarcinoma in the experimental group was significantly higher than that in the control group(P0. 05). Conclusion The methylene blue chromoendoscopy has high detection rate for early gastric cancer and precancerous lesion and is conducive to find and diagnose early gastric cancer and precancerous lesion with the advantages of simple method, safety and practicability.%目的 探讨色素内镜对早期胃癌及癌前病变的诊断价值.方法 将2010年1月至2011年12月该院消化内科门诊及住院患者194例,根据不同内镜检查分为实验组和对照组,104例行亚甲蓝色素内镜检查的为实验组,对照组90例行普通胃镜检查,比较亚甲蓝色素内镜和普通内镜的早期胃癌及癌前病变的检出率.结果 实验组腺上皮肠化、不典型增生及腺癌检出率明显高于对照组(P<0.05);而腺上皮增生和腺瘤检出率两组比较,差异无统计学意义(P>0.05).结论 亚甲蓝色素内镜对早期胃癌及癌前病变的检出率更高,有助于早期胃癌及癌前病变的发现及诊断,具有方法简单、安全、实用的特点.

  4. 色素内镜在上消化道早期癌及癌前病变的诊断价值%Diagnostic value for early cancer and precancerous lesions in the digestive tract under pigment endoscopy

    Institute of Scientific and Technical Information of China (English)

    陈函清; 潘文胜; 金冠福; 潘邦兴

    2012-01-01

    目的 研究色素内镜对上消化道早期癌及癌前病变的诊断价值.方法 将内镜下216例食管黏膜病变患者分为两组,染色组108例用Lugol氏液对食管黏膜染色,并对不染色和浅染色区进行病理活检;对照组108例食管黏膜患者进行单纯病理活检.将内镜下220例胃黏膜病变患者分为两组,染色组110例用靛胭脂一美兰染色后进行病理活检,对照组110例胃黏膜病变患者进行单纯病理活检.结果 食管染色组不染色或浅染色病例60例(55.6%),病理活检发现早期食管癌12例(11.1%),癌前病变6例(5.6%);胃染色组有69例(62.7%)不同程度染色,病理活检发现早期胃癌14例(12.7%),其中原位癌4例(3.6%),癌前病变9例(8.1%).发现早期癌及癌前病变与对照组的诊断率比较差异有显著性(P<0.01).结论 色素内镜有助于提高上消化道早期癌及癌前病变的诊断率及病理活检准确率,方法简便安全,值得基层医院推广.%[Objective] To study diagnostic value of early cancer and precancerous lesions in upper gastrointestinal under pigment endoscopy. [Methods] 216 cases of endoscopic esophageal mucosal lesions, staining group of 108 cases were treated with Lugol's solution staining of the esophageal mucosa, and non-staining and light staining zone biopsy; Control group, 108 cases of esophageal mucosa in patients with a simple biopsy. 220 cases of endo- scopic gastric mucosal lesions, indigo dyeing group of 110 cases with rouge - methylene blue staining and biopsy in the control group of 110 cases gastric mucosal lesions in patients with a simple biopsy. [Results] Esophagus didn't stain or stained pale group of 60 cases (55.6%), biopsy of 12 early esophageal cancer (11.1%), precancerous lesions in 6 patients (5.6%); Stomach stained group of 69 patients (62.7%) varying degrees of staining, biopsy detection of 14 early gastric cancer (12.7%), which carcinoma in situ in 4 cases, 9 cases of precancerous

  5. Expression and clinical significance of E-cadherin,β-catenin and E-cadherin-catenins complex in breast cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Zuofeng Zhang; Shuguang Yang; Gangping Wang

    2014-01-01

    Objective: The aim of our study was to observe the expressions and clinical Significance of E-cadherin, β-catenin and E-cadherin-catenins complex in breast cancer and precancerous lesions, and analyze the relationship between the ex-pressions and clinicopathological features in breast cancer. Methods: Immunhistochemical UltraSensitiveTM S-P method was employed to detect the expression of E-cadherin, β-catenin and E-cadherin-catenins complex in 128 cases of invasive ductal carcinomas, 89 cases of ductal carcinoma in situ and 57 cases of atypical ductal hyperplasia, 53 cases of usual ductal hyper-plasia breast tissues were selected as a control group. The express of E-cadherin, β-catenin and their relationship with mult biological parameters including histological grade, region lymph node metastasis, distant metastasis and recurrence on files were also assessed. Results: (1) The staining patterns character of E-cadherin, β-catenin and E-cadherin-catenins complex:In UDH breast tissues, E-cadherin and a-catenin were expressed on cellmembrane of ductal and acinic cells, showing cellular contour and border among cells. The abnormal expression of the three proteins occurred in breast invasive ductal carcinomas, ductal carcinoma in situ and atypical ductal hyperplasia tissues, showing cytoplasmic or nuclear staining, decrease and loss of cytomembrane staining. (2) The abnormal expression rates of E-cadherin, β-catenin and E-cadherin-catenins complex in invasive ductal carcinomas were 53.91%, 65.63% and 81.25%, which were significantly higher than that in ductal carcinoma in situ, atypical ductal hyperplasia, usual ductal hyperplasia tissues (P 0.05). (3) There was a significantly dif erence in the mean E-cadherin, β-catenin and E-cadherin-catenins complex frequency between estrogen receptor & progesterone receptor positive IDC group and negative group, epidermal growth factor receptor type 2 (HER2/neu) positive and negative groups, Ki-67 proliferation index ≤ 14

  6. Cervical intraepithelial lesions in females attending Women's Health Clinics in Alexandria, Egypt

    OpenAIRE

    Mona Abdel-Hadi; Adel Khalaf; Hanaa Aboulkassem; Noha Naeem; Mohamed Abdel Baqy; Hassan Sallam

    2015-01-01

    Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN)   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to hav...

  7. Clinical study on colposcopy combined with cervical smear in detection of cervical lesions%阴道镜联合宫颈涂片检测宫颈病变的临床研究

    Institute of Scientific and Technical Information of China (English)

    周鸿; 朱瑾

    2012-01-01

    CIN 1,9 cases with CIN H , and 6 cases with CIN 01; the coincidence rate of colposcopic RCI score and pathological examination based on colposcopic biopsy was 94. 74% (36/38) . Twenty -four cases was suspected of CIN II by cervical cytological smear, 14 cases were confirmed by pathological examination based on colposcopic biopsy, the coincidence rate was 58. 33% (14/24) . There was significant difference in the coincidence rate of CIN between colposcopic RCI score and cervical cytological smear ( P < 0. 05 ) . The sensitivity, specificity, positive predictive value, and negative predictive value were 95. 50% , 89. 26% , 72.21% , and 95. 85% , respectively. Conclusion: Cervical cytological smear combined with colposcopy used in cervical lesions screening can improve the diagnostic rates of cervical precancerous lesion and early cervical cancer, which can be used as one of the feasible methods to screen cervical precancerous lesion in the region.

  8. Location and Density of Immune Cells in Precursor Lesions and Cervical Cancer.

    Science.gov (United States)

    Bedoya, Astrid M; Jaramillo, Roberto; Baena, Armando; Castaño, Jorge; Olaya, Natalia; Zea, Arnold H; Herrero, Rolando; Sanchez, Gloria I

    2013-04-01

    Only a small proportion of women infected with Human Papillomavirus (HPV) develop cervical cancer. Host immune response seems to play a role eliminating the viral infection and preventing progression to cancer. Characterization of tumor infiltrating lymphocytes (TILs) in cervical pre-neoplastic lesions and cervical cancer may be helpful to understand the mechanisms that mediate this protection. The aim of this study was to determine if there are differences in the localization and density (cells/mm(2)) of CD8+ T-cells, CD4+ T-cells and Tregs (CD25 + Foxp3+) in cervical pre-neoplastic lesions and cervical cancer. Immunohistochemical analysis of sections of 96 (26 CIN1, 21 CIN2, 25 CIN3, and 24 SCC) samples revealed that regardless of CIN grades, CD8+ T-cells are more abundant than CD4+, CD25+ and Foxp3+ cells in both the stroma and epithelium. There was a higher density of CD8+ cells in the stroma of cervical cancer compared to CIN3 (OR = 4.20, 95% CI 1.2-15), CIN2 (OR = 7.86, 95% CI 1.7-36.4) and CIN1 (OR = 4.25, 95% CI 1.1-17). Studies evaluating whether these cells are recruited before or after cancer progression will be helpful to understand the role of these cells in the natural history of HPV-induced lesions.

  9. Human papillomavirus infection in couples with female low-grade intraepithelial cervical lesion.

    Science.gov (United States)

    Simon, Philippe; Roumeguere, Thierry; Christophe Noël, Jean

    2010-11-01

    Low-grade squamous intraepithelial lesions (LSIL) are frequently found during cervical cancer screening. Usually they are associated with a human papillomavirus (HPV) infection. Does the high-transmission rate of HPV infection to the male partner represent a clinical risk for him? Are preventive measures to be taken to prevent the occurrence of male diseases? More than 80% of all LSIL are associated with HPV infections. The prevalence of HPV infection in males can range up to 40%, with 60% of the male partners of LSIL female patients presenting with penile flat lesions. The spontaneous cure rate for male infections is very high (90% at 5 years) but negative consequences in females (cervical high-grade lesion and cervical cancer) are frequent. Their male counterparts are far rarer but in some patients can require deleterious treatment. Transmission prevention by the use of condoms and circumcision is discussed. The effectiveness of HPV vaccination in this situation has not been validated. PMID:20646823

  10. Research progress in relationship between HPV testing and diagnosis and treatment of cervical lesions%HPV检测在宫颈疾病诊治中的研究进展

    Institute of Scientific and Technical Information of China (English)

    景笑笑; 杨雪峰

    2012-01-01

    Cervical cancer is the most common malignant tumor in female reproductive system, and its etiology is not entirely clear. Currently it is accepted that cervical cancer is related with human papilloma virus ( HPV ) infection. The persistent high - risk HPV infection is the main cause of cervical lesions. The detecting and therapy of high-risk HPV has become an important means in screening precancerous cervical diseases and preventing cervical cancer. This article summarized the relationship between HPV infection and cervical lesions, relationship between HPV viral load and classification and severity of cervical disease, the clinical value of HPV in evaluating therapeutic efficacy, judging prognosis and guiding cytology positive patients in shunt aspects and HPV vaccine.%宫颈癌是女性生殖系统最常见的恶性肿瘤,其发病原因尚未完全清楚,目前公认宫颈癌的发病与人乳头瘤病毒(HPV)感染有关,持续的高危型HPV感染是引起宫颈病变的主要病因,对高危型HPV的检测和治疗已成为筛查宫颈癌前病变、预防宫颈癌的重要手段.该文围绕HPV感染与宫颈病变的关系,HPV病毒载量、分型与宫颈病变严重程度的关系以及其在评估治疗效果、判断预后、指导细胞学阳性患者分流等方面的临床价值,HPV疫苗等的研究进展作以综述.

  11. RESEARCH PROGRESS OF SCREENING METHOD OF CERVICAL INTRAEPITHELIAL NEOPLASIA%宫颈上皮内瘤变筛查方法的研究进展

    Institute of Scientific and Technical Information of China (English)

    张俊绘; 宋静慧

    2011-01-01

    宫颈癌是女性最常见的恶性肿瘤之一,从癌前病变发展到癌大约需要10a时间,及早发现癌前病变对宫颈癌的防治具有重要意义.目前筛查宫颈癌前病变的方法有液基细胞学检测、HPV-DNA检测、阴道镜检查、组织病理学检查等.综述宫颈癌前病变的筛查方法,探讨最为高效合理的宫颈癌的筛查方法,提高诊断的准确率,从而降低宫颈癌的发病率和死亡率.%Cervical cancer is one of the most common cancers of women, it will take about 10 years from precancerous lesion to cancer, early detection of precancerous lesion is significant for prevention and cure of cervical cancer. At present, the methods of screening precancerous lesions of cervical cancer mainly include thin prep liquid based cytology test, HPV - DNA test, colposcopy examination , histopathology examination, etc. This article summarized the screening methods of precancerous lesions of cervical cancer, seek the most effective and rational screening methods of cervical cancer , improve the accuracy of diagnosis, so as to decrease the incidence and mortality of cervical cancer.

  12. Endoscopic Submucosal Dissection for Early Esophageal Cancer and Esophageal Precancerous Lesions%内镜黏膜下剥离术治疗早期食管癌及食管癌前病变

    Institute of Scientific and Technical Information of China (English)

    薛鹏星; 周旋光; 陈娟

    2012-01-01

    Objective The efficacy of endoscopic submucosal dissection (ESD) in the treatment of early esophageal cancer and esophageal precancerous lesions was studied. Methods 4 patients with early esophageal cancer and 21 patients with esophageal precancerous lesions were treated by ESD after confirming the lesion range and invasion depth. Endoscopic ultrasonography was used to measure the invasion depth and lugol's staining was applied in measuring lesion range. Intraoperative mapping, submucosal injection, marginal mucosa incision, split and wound treatment were utilized as well. Results All lesions were completely removed, and no perforation or bleeding occurred during the operation. In the third month one patient was found to have esophageal stenosis which was relieved after a dilation. To random visit the patients 3, 6 and 12 months after operation, there was no recurrence. Conclusion ESD is a safe way to treat early esophageal cancer and esophageal precancerous lesions.%研究内镜黏膜下剥离术(ESD)治疗早期食管癌及食管癌前病变的治疗价值。对25例早期食管癌及食管癌前病变在确定病变范围及浸润深度后行黏膜下剥离术。先超声内镜判断浸润深度及利用Lugol液染色确定病灶范围,后采用术中标记、粘膜下注射、边缘粘膜切开、剥离及创面处理等方法。结果显示,病灶完整切除,术中无穿孔、出血;术后3、6、12月常规随访,均无复发。1例术后3个月出现食管狭窄,给予扩张后狭窄缓解。结果表明,对早期食管癌及食管癌前病变,内镜黏膜下剥离术是一种安全的内镜下治疗方法。

  13. Dinamics of HPV Infection among Women with Cervical Lesions

    OpenAIRE

    E. Nikitina; Urazova, L.; Churuksaeva, O.

    2011-01-01

    A total of 293 women treated at Tomsk Cancer Research Institute were examined. HPV type 16 had the highest incidence rate (45.0%) followed by HPV 31-17,0%, HPV 56/33-15,0%, HPV 51/18/52-13,0%, HPV 58/35/39/45-7,0%, HPV 59-5,0%. Persistent infection was detected in 35.7% of primarily HPV-positive cases (10 out of 28 patients), mainly in cervical cancer patients. Total number of primarily HPV-positive and HPV-negative patients with cervical cancer was 95.0% and 5.0%, respectively. The correspon...

  14. Chromoendoscopy(Lugol's Solution) for Diagnosis of Early Esophageal Cancer and Precancerous Lesions%胃镜下卢戈液染色对早期食管癌及癌前病变的诊断

    Institute of Scientific and Technical Information of China (English)

    李小华; 罗忠金; 方慧祺; 何洁; 邓燕

    2011-01-01

    目的 探讨胃镜下卢戈液染色诊断早期食管癌及癌前病变的价值.方法 对40例胃镜下食管有可疑病变者行卢戈液染色,观察着色情况并取材常规送病理检查.结果 40例卢戈液染色有24例(36处)不着色区或淡染区,经病理诊断食管黏膜内癌2处,不典型增生20处,Barrett食管8处,单纯增生6处.结论 胃镜下卢戈液染色能提高早期诊断食管癌及癌前病变的水平.%Objective To discuss the value of chromoendoscopy (Lugol's solution) for the diagnosis of early esophageal cancer and precancerous lesions.Methods Chromoendoscopy with Lugol's solution was used in the esophagus to detect lesions in 40 patients with suspicious esophageal lesions, and biopsy specimens were taken from the lesions for pathologic diagnosis.Results Among the 40 cases,24 (36 regions) were not stained with Lugol' s solution.Pathologic examination showed esophageal mucous menbrane carcinoma in 2 cases, atypical hyperplasia in 20, Barrett's esophagus in 8 and simple hyperplasia in 6.Conclusion Chromoendoscopy with Lugol's solution can elevate diagnostic levels of early esophageal cancer and precancerous lesions.Therefore,it is worthy to be widely used in clinical practice.

  15. Immunohistochemical expression of p16ink4a in inflammatory, preneoplastic and neoplastic cervical lesions

    Directory of Open Access Journals (Sweden)

    Gajanin Radoslav

    2015-01-01

    Full Text Available Introduction. High-risk human papilloma viruses play a main role in the development of cervical dysplasias and carcinomas. p16INK4a can be considered as a surrogate marker of active highrisk human papillomaviruses infection in dysplastic and neoplastic cells of the cervix. This study was aimed at determining the presence and level of p16INK4a expression in inflammatory, preneoplastic and neoplastic lesions of the cervix. Material and Methods. The study was performed on 109 samples of cervical biopsy. Cervical cancer was diagnosed in 36 patients, 34 patients had a preneoplastic change (dysplasia in stratified squamous cervix epithelium and a nonspecific inflammatory process was found in 39 patients. In all samples, immunohistochemical analysis using antibodies to p16INK4a was performed. Results. The expression of p16INK4a was verified in all cases of cervical cancer (100%, in 67.65% of dysplastic cervical lesions and in 38.5% of inflammatory lesions. A statistically highly significant difference was found in the presence and level of expression among neoplasic, dysplastic and inflammatory lesions of the cervix (χ² = 76.02, p < 0.001. The expression was more frequent and had a higher level in neoplastic and high grade dysplastic lesions compared to expression in inflammatory lesions and low grade dysplasias. Conclusion. The analysis of the presence of p16INK4a can differentiate non-neoplastic, high grade preneoplastic and neoplastic changes of the cervix. The use of p16INK4a in interpreting borderline lesions of the cervix can enable a rational therapeutic treatment of patients.

  16. Quantitative Detection of Screening for Cervical Lesions with ThinPrep Cytology Test

    Institute of Scientific and Technical Information of China (English)

    Hong-xin ZHANG; Yi-min SONG; Su-hong LI; Yu-hui YIN; Dong-ling GAO; Kui-sheng CHEN

    2010-01-01

    OBJECTIVE To investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology (ThinPrep Cytology Test, TCT) and The Bethesda System (TBS), also with computer image analysis. METHODS With application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted in atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells-cannot exclude HSIL (ASC-H), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma (SCC) with the mean optical density (MOD), average grey (AG), positive units (PU), and nucleus to cytoplasmic ratio (N:C). Differences between each group of cells were compared and analyzed statistically.RESULTS Apart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups, there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of nucleus to cytoplasm were highly statistically signifi cant. CONCLUSION Stereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become a new means of cytological assisted diagnosis.

  17. 5-year clinical performance of resin composite versus resin modified glass ionomer restorative system in non-carious cervical lesions

    DEFF Research Database (Denmark)

    Franco, Eduardo Batista; Benetti, Ana Raquel; Ishikiriama, Sérgio Kiyoshi;

    2006-01-01

    To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions.......To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions....

  18. 喉癌前病变中医证候及四诊特征初步研究%Pilot Study on Chinese Medicine Pattern/Syndrome and the Characteristics of Four Diagnostic Methods for Laryngeal Precancerous Lesions

    Institute of Scientific and Technical Information of China (English)

    卢标清; 李云英; 孙一帆; 周小军; 李少华; 陈子清; 郭亿莲; 伍映芳

    2012-01-01

    目的 通过临床调查探讨喉癌前病变的中医证候及四诊特点,为喉癌前病变的中医药防治及研究提供参考和思路.方法 采用流行病学横断面调查方法,对88例喉癌前病变、100例声带息肉及60例喉癌患者的中医证候及四诊资料进行采集分析.结果 在声带息肉→喉癌前病变→喉癌的病情进展过程中,中医证候演变特点:以痰瘀为主线,证候由单一向证候兼杂发展,最终出现痰瘀热毒、气阴两虚等多种证候因素兼杂的情况.舌象变化特点:随着病情的发展变化,舌象可由正常的淡红舌为主,向暗红舌、红绛舌、紫舌等发展;舌苔由淡白、白腻向薄黄、黄腻到黄老厚腻变化发展;舌边瘀斑瘀点及舌下静脉曲张的情况更普遍.结论 痰瘀是喉癌前病变的基本病因病机,贯穿于喉癌前病变发生发展的始终.%Objective To clinically investigate and explore the Chinese medicine pattern/syndrome and the characteristics of the four diagnostic methods of laryngeal precancerous lesions so as to provide the references and thinking approaches to the prevention,treatment as well as the research of laryngeal precancerous lesions with traditional Chinese medicine. Methods The cross - sectional investigation of epidemiology was adopted to collect and analyze the Chinese medicine pattern/syndrome and the characteristics of four diagnostic methods for 88 cases of laryngeal precancerous lesions, 100 cases of vocal cord polyp and 60 cases of laryngocarcinoma. Results In the sickness progression, vocal cord polyp → laryngeal precancerous lesions → laryngocarcinoma,the characteristics of the evolution of the Chinese medicine pattern/syndrome indicated: The phlegm stasis pattern/syndrome was essential and the disorders developed more and more complicated. Finally the mixed pattern/syndrome occurred,such as phlegm stasis mixed with heat toxin,and deficiency of qi and yin. The characteristics of tongue conditions

  19. Performance Evolution of Various Wavelets in Cervical Lesion Detection

    Directory of Open Access Journals (Sweden)

    P S RAMAPRABA

    2014-01-01

    Full Text Available Cervical cancer is one of most common cancers among women in the world caused by human papilloma virus infection. It develops in the tissue of cervix which connects upper body of the uterus to the vagina. The types of cancer are squamous cell carcinoma, adeno carcinoma and adeno squamous carcinoma based on location of cervix where cancer develops. In this paper, an automatic detection of squamous cell carcinoma in cervical images based on Discrete Wavelet Transform (DWT and K-Nearest Neighbor (KNN classifier is described. The energy features are extracted from DWT decomposed image of small area of cervical images. Then the features are fed into KNN classifier to classify whether the given area is normal or cancer affected region. The performance of the proposed system is evaluated by using three wavelets namely bi-orthogonal (bior3.7, Daubechies-8(db8 and Symlet (sym8. Experimental results show the performance of db8 with other wavelets that produces 97.22% average accuracy.

  20. A study of metastatic lesions of cervical lymphadenopathy by fine needle aspiration cytology

    Directory of Open Access Journals (Sweden)

    Deepa Jethwani

    2015-07-01

    Conclusion: FNAC is a cost effective, reliable, rapid and inexpensive method for diagnosis of lymphadenopathy. Cytology of Metastatic lesions in cervical lymph nodes gives clue to nature and origin of tumors. [Int J Res Med Sci 2015; 3(7.000: 1697-1700

  1. Prevalence of human papillomavirus DNA in female cervical lesions from Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    S. M. B. Cavalcanti

    1994-12-01

    Full Text Available A hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (HPV types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. The data were compared with histologic diagnosis. Eighty-eight (55 biopsies contained HPV DNA sequences. In low grade cervical intraepithelial neoplasias (CIN I, HPV infection was detected in 78.7 of the cases, the benign HPV 6 was the most prevalent type. HPV DNA was detected in 58 of CIN II and CIN III cases and in 41.8 of squamous cell carcinomas (SCC. Histologically normal women presented 20 of HPV infection. Oncogenic HPV was found in 10 of these cases, what may indicate a higher risk of developing CINs and cancer. Twenty-five percent of the infected tissues contained mixed infections. HPV 16 was the most common type infecting the cervix and its prevalence raised significantly with the severity of the lesions, pointing its role in cancer pathogenesis. White women presented twice the cervical lesions of mulatto and African origin women, although HPV infection rates were nearly the same for the three groups (approximately 50. Our results showed that HPV typing by in situ hybridization is a useful tool for distinguishing between low and high risk cervical lesions. Further studies are required to elucidate risk factors associated with HPV infection and progression to malignancy in Brazilian population.

  2. One-year clinical evaluation of tooth-coloured materials in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Stojanac Igor

    2011-01-01

    Full Text Available Introduction. The restoration of non-carious cervical lesions is specific, mostly because of the location of their margins, especially the cervical margin, which is determined in cement and/or dentine. This feature makes the cervical margin more susceptible to micro-leak­age, causing marginal discoloration, postoperative sensitivity, development of the secondary caries and loss of the restoration. Material and methods. One of the criteria for inclusion of a patient in this study was the presence of at least two non-carious cervical lesions to be restored with the minimum depth of 1mm, independently of their location in the dental arch. A total of 60 restorations were placed in 30 patients, and every patient received both tested materials (composite resins and compomer on their non-carious cervical lesions. The clinical evaluation of the therapeutic success was performed six months and then one year after the day of the placement of restorations using the modified-United States Public Health Service criteria. The following was evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. Results. A statistically significant high percent of restorations/teeth with postoperative sensitivity was found in the group of resin composite restorations after six months. At the end of the evaluation period, that is after one year, there were no statistically significant differences between materials for all evaluated criteria. Conclusion. The results of this study show the identical quality of both examined materials one year after the readjustment of non-carious cervical lesions.

  3. Percutaneous anterolateral balloon kyphoplasty for metastatic lytic lesions of the cervical spine.

    Science.gov (United States)

    Lykomitros, Vasilis; Anagnostidis, Kleovoulos S; Alzeer, Ziad; Kapetanos, George A

    2010-11-01

    The purpose of our report is to describe a new application of kyphoplasty, the percutaneous anterolateral balloon kyphoplasty that we performed in two cases of metastatic osteolytic lesions in cervical spine. The first patient, aged 48 years, with primary malignancy in lungs had two metastatic lesions in C2 and C6 vertebrae. Patient's complaints were about pain and restriction of movements (due to the pain) in the cervical spine. The second patient, aged 70 years, with primary malignancy in stomach, had multiple metastatic lesions in thoracolumbar spine and C3, C4 and C5 vertebrae without neurological symptoms. The main symptoms were from cervical spine with severe pain even in bed rest and systematic use of opiate-base analgesic. The preoperative status was evaluated with X-rays, CT scan, MRI scan and with Karnofsky score and visual analogue pain (VAS) scale. Both patients underwent percutaneous anterolateral balloon kyphoplasty via the anterolateral approach in cervical spine under general anaesthesia. No clinical complications occurred during or after the procedure. Both patients experienced pain relief immediately after balloon kyphoplasty and during the following days. The stiffness also resolved rapidly and cervical collars were removed. VAS score significantly improved from 85 and 95 preoperatively to 30 in both patients. Karnofsky score showed also improvement from 40 and 30 preoperatively to 80 and 70, respectively, at the final follow-up (7 months after the procedure). Fluoroscopy-guided percutaneous anterolateral balloon kyphoplasty proved to be safe and effective minimally invasive procedure for metastatic osteolytic lesions of the cervical spine, reducing pain and avoiding vertebral collapse. Experience and attention are necessary in order to avoid complications.

  4. The 836 Cases of Cervical Cancer Screening Lesions Result Analysis%宫颈癌前期病变筛查836例结果分析

    Institute of Scientific and Technical Information of China (English)

    杨惠新

    2012-01-01

    Objective:To summarize and analyze the results of precancerous lesion screening for cervical caner and provide evidence for cervical caner prevention.Methods:The characteristics such as age,education level,birth history and cervical laceration,episiotomy of the 836 participants were reviewed,the results of LCT and histopathologic diagnosis were compared,and the LCT results of foreign female labors and local females were also compared and analyzed.Results:The positive rate of LCT was 10.77%,the accuracy was 89.89%.The proportions of history of birth,episiotomy and cervical laceration in LCT positive participants were statistically significantly higher than in the LCT negative participants (P<0.05).Positive rate of LCT in foreign female labors was statistically higher than in local females (P<0.05). Conclusion:LCT is a ideal screening tool for cervical cancer in this area,gynecologic health care education should be enhanced in foreign female labors to improve the activity and compliance for participating the cervical cancer screening.%目的:总结分析宫颈癌前期病变LCT筛查结果,为本地区宫颈癌预防提供依据.方法:回顾调查836例参加宫颈癌前期病变LCT筛查妇女的年龄、文化程度、分娩史和宫颈撕裂、会阴切除术等病史,对比分析LCT检查及其与组织病理学诊断结果,对比分析外来务工妇女与本地妇女参加检查情况和检查结果.结果:LCT阳性率为10.77%,准确率为89.89%.LCT阳性者,有分娩、会阴切开术、宫颈撕裂史比例高于LCT阴性者,差异具有统计学意义(P<0.05).外来务工妇女LCT检出率高于本地居民,差异具有统计学意义(P<0.05).结论:LCT是本地区较为理想的宫颈癌筛查方法;应大力加强外来务工妇女妇科保健宣传力度,提高其参加宫颈癌筛查的主动性和依从性.

  5. Comparison of MRI pulse sequences for investigation of lesions of the cervical spinal cord

    Energy Technology Data Exchange (ETDEWEB)

    Campi, A.; Pontesilli, S.; Gerevini, S.; Scotti, G. [San Raffaele Hospital, Milan (Italy). Dept. of Neuroradiology

    2000-09-01

    Small spinal cord lesions, even if clinically significant, can be due to the low sensitivity of some pulse sequences. We compared T2-weighted fast (FSE), and conventional (CSE) spin-echo and short-tau inversion-recovery (STIR)-FSE overlooked on MRI sequences to evaluate their sensitivity to and specificity for lesions of different types. We compared the three sequences in MRI of 57 patients with cervical spinal symptoms. The image sets were assessed by two of us individually for final diagnosis, lesion detectability and image quality. Both readers arrived at the same final diagnoses with all sequences, differentiating four groups of patients. Group 1 (30 patients, 53 %), with a final diagnosis of multiple sclerosis (MS). Demyelinating lesions were better seen on STIR-FSE images, on which the number of lesions was significantly higher than on FSE, while the FSE and CSE images showed approximately equal numbers of lesions; additional lesions were found in 9 patients. The contrast-to-noise ratio (CNR) of 17 demyelinating lesions was significantly higher on STIR-FSE images than with the other sequences. Group 2, 19 patients (33 %) with cervical pain, 15 of whom had disc protrusion or herniation: herniated discs were equally well delineated with all sequences, with better myelographic effect on FSE. In five patients with intrinsic spinal cord abnormalities, the conspicuity and demarcation of the lesions were similar with STIR-FSE and FSE. Group 3, 4 patients (7 %) with acute myelopathy of unknown aetiology. In two patients, STIR-FSE gave better demarcation of lesions and in one a questionable additional lesions. Group 4, 4 patients (7 %) with miscellaneous final diagnoses. STIR-FSE had high sensitivity to demyelinating lesions, can be considered quite specific and should be included in spinal MRI for assessment of suspected demyelinating disease. (orig.)

  6. Application of confocal laser endomicroscopy in diagnosis of digestive tract cancer and precancerous lesions%共聚焦激光显微内镜在消化道肿瘤及癌前病变中的应用

    Institute of Scientific and Technical Information of China (English)

    杨竞; 范楠楠; 杨云生

    2012-01-01

    Confocal laser endomicroscopy (CLE) is a novel and advanced endoscopic technique, which can clearly display the structures of the mucosal surface and subsurface during ongoing endoscopy, thereby providing immediate diagnosis of mucosal lesions. CLE can distinguish the physiological change from the pathological in the gastrointestinal mucosa by using a fluorescent contrast agent, which demonstrates its potential in diagnosis of gastrointestinal diseases. A series of studies have shown that CLE has high specificity and sensitivity in diagnosis and distinction of gastrointestinal tumors, especially the early cancer and precancerous lesions. The present paper will focus on advances in researches about CLE in gastrointestinal tumors and precancerous lesions.%共聚焦激光显微内镜(CLE)是一项在常规内镜检查同时能清晰地显示黏膜表面及表面下组织结构,从而对黏膜病变做出即时诊断的全新的内镜技术.CLE在荧光对比剂作用下能够对胃肠道黏膜的病理生理改变进行准确诊断,显示了其用于消化道疾病诊断的潜能.一系列研究表明CLE对消化道肿瘤尤其是早期肿瘤及癌前期病变的诊断及鉴别诊断具有较高的特异性和敏感性,本文就其在消化道肿瘤及癌前病变中的应用进展做一综述.

  7. Skin cancer and precancerous skin lesions:clinical analysis of 632 cases%632例皮肤恶性肿瘤及癌前病变临床分析

    Institute of Scientific and Technical Information of China (English)

    黄远深; 李航; 涂平; 陈喜雪; 杨淑霞; 武玲慎; 赵俊郁; 李雪迎

    2010-01-01

    目的 探讨皮肤恶性肿瘤的临床特点.方法 回顾2005-2008年病理诊断皮肤恶性肿瘤及癌前病变的病例资料,重新阅片确认肿瘤诊断和病理亚型分型后,利用统计软件进行分析.结果 基底细胞癌及原位和侵袭性鳞状细胞癌最多见,分别占总病例数的29.3%及24.2%.在所研究病例中,>60岁发病者占55.4%,35~59岁发病比例达到34.3%,青少年患者比例为10.3%.Paget病临床诊断与病理诊断的符合率接近90.0%,但其他常见皮肤恶性肿瘤及癌前病变临床与病理的符合率仅在70.0%左右.结论 在皮肤恶性肿瘤及癌前病变中,头面部皮肤肿瘤及癌前病变病例明显多于其他部位.中青年患者不仅在皮肤淋巴瘤相关肿瘤病例中多见,而且在上皮来源的皮肤恶性肿瘤及黑素瘤病例中占有显著比例.%Objective To investigate the clinical features of skin cancer.Methods Clinical data of skin cancer and precancerous skin lesions confirmed pathologically from 2005 to 2008 in Peking University First Hospital were retrospectively analyzed by using statistical methods.Results A total of 632 cases of skin cancer and precancerous skin lesions were studied.The most common skin cancer was basal cell carcinoma and squamous cell carcinoma (invasive and in situ) which accounted for 29.3%and 24.2%,respectively.The average age at onset was older than 60 years in 55.4%of the patients,between 35 and 59 years in 34.3%,younger than 35 years in 10.3%.The concordance between clinical and pathological diagnosis reached nearly 90.O%for Paget's disease,70.0% for other common skin cancer and precancerous skin lesions.Conclusions Skin cancer and precancerous skin lesions have a predilection for scalp and face.Patients aged from 35 to 59 years account for a significant proportion not only in cutaneous lymphoma but also in melanoma and epithelium-derived nonmelanoma skin cancer.

  8. Cervical cancer: screening, diagnosis and staging.

    Science.gov (United States)

    Tsikouras, Panagiotis; Zervoudis, Stefanos; Manav, Bachar; Tomara, Eirini; Iatrakis, George; Romanidis, Constantinos; Bothou, Anastasia; Galazios, George

    2016-01-01

    Purpose: Despite the widespread screening programs, cervical cancer remains the third most common cancer in developing countries. Based on the implementation of cervical screening programs with the referred adoption of improved screening methods in cervical cytology with the knowledge of the important role of the human papilloma virus (HPV) it's incidence is decreased in the developed world. Even if cervical HPV infection is incredibly common, cervical cancer is relatively rare. Depending on the rarity of invasive disease and the improvement of detection of pre-cancerous lesions due to the participation in screening programs, the goal of screening is to detect the cervical lesions early in order to be treated before cancer is developed. In populations with many preventive screening programs, a decrease in cervical cancer mortality of 50-75% is mentioned over the past 50 years. The preventive examination of vagina and cervix smear, Pap test, and the HPV DNA test are remarkable diagnostic tools according to the American Cancer Association guidelines, in the investigation of asymptomatic women and in the follow up of women after the treatment of pre-invasive cervical cancer. The treatment of cervical cancer is based on the FIGO 2009 cervical cancer staging.

  9. 内镜色素染色对早期胃癌及癌前病变的诊断价值研究%The value of methylene blue staining in diagnosis of precancerous lesions and early gastric cancer

    Institute of Scientific and Technical Information of China (English)

    何东凌; 冯青

    2009-01-01

    Objective To evaluate the value of methylene blue staining in diagnosis of precancerous lesions and early gastric cancer.Methods There are 224 paients with abnormal gastric mucous membrance,104 cases were biopsy after stain by Methylene blue and 120 cases were taken biopsy simplicity.Results One hundred and four suspected early careinonm of stomach cases were stain by Methylene blue and 81 eases were found dyeing.There are 75 patients with atypical hyperplasia or intestinal metaplasi,6 with early gastric cancers,4 with preinvasive carcinoma.One hundred and twenty patients were taken biopsy simplicity.There are 14 patients with mild to moderate atypical hyperplasia,36 cases of chronic gastritis with focal intestinal metaplasia.Conclusions The early diagnosis rate of precancerous lesions and early gastric cancer are very well improved by methylene blue staining.%目的 评价和探讨内镜色素染色对早期胃癌及癌前病变的诊断价值.方法 对224例胃镜下粘膜异常表现患者,104例局部美蓝染色后活检,120例单纯活检.结果 美蓝染色组104例,有81例有不同程度染色,病理示不典型增生及肠上皮化生75例,早期胃癌6例,其中原位癌4例.单纯活检组120例,14例存在轻度至中度不典型增生,36例慢性胃炎伴肠化局灶性肠化.结论 内镜色素染色有助于提高早期胃癌及癌前病变的早期诊断.

  10. Assessment of quality of life for the patients with cervical cancer at different clinical stages

    Institute of Scientific and Technical Information of China (English)

    Yao Xie; Fang-Hui Zhao; Si-Han Lu; He Huang; Xiong-Fei Pan; Chun-Xia Yang; You-Lin Qiao

    2013-01-01

    With improved overall survival of cervical cancer patients,the importance of the quality of life (QOL)is increasingly recognized.This study was conducted to compare the QOL of women with different stage cervical cancer before and after treatment to facilitate improved cervical cancer prevention and treatment.We used the generic Medical Outcomes Study Short Form-36 (MOS SF-36) to collect QOL information.Based on SF-36,we interviewed cervical cancer patients at West China Second Affiliated Hospital and Sichuan Cancer Hospital between May 2010 and January 2011.A total of 92 patients with precancerous lesions,93 with early cancer,and 35 with advanced cancer responded to our survey.Average physical component summary (PCS) scores were significantly different between the three groups at every time point (P < 0.05).Average mental component summary (MCS) scores were significantly different between the three groups after treatment (P < 0.05).Average PCS and MCS scores increased gradually from the pretreatment to posttreatment period for patients with precancerous lesions.However,they reached the lowest at 1 month after treatment for patients with early and advanced cancers and rebounded between 1and 6 months after treatment.Our results indicate that patients with precancerous lesions and early cervical cancer show better overall QOL than do those with advanced cervical cancer.Additionally,patients with early cancer recover more quickly than do those with advanced cancer in terms of both physical and mental functions.Thus,early detection and treatment initiatives may improve the QOL for patients with precancerous lesions and cervical cancer.

  11. Extracranial Vertebral Artery Aneurysm Presenting as a Chronic Cervical Mass Lesion

    Directory of Open Access Journals (Sweden)

    Lampis C. Stavrinou

    2010-01-01

    Full Text Available Background. Aneurysms of the extracranial vertebral artery are rare and can provide a diagnostic and therapeutic challenge. Methods. We reviewed the clinical history of a patient presenting with cervical radiculopathy, who harboured an extracranial vertebral artery aneurysm eroding the cervical spine. Results. CT Angiography and MR Angiography set the diagnosis, by revealing a left C5-C6 vertebral artery aneurysm with cervical root impingement. Bony reconstruction depicted enlargement of the C6 transverse foramen and a marked enlargement of the C6-C7 intravertebral foramen. The lesion was treated by intravascular proximal vertebral artery occlusion. Conclusions. Extracranial vertebral artery aneurysms require a high index of clinical suspicion. This is the first report of a vertebral artery pseudoaneurysm presenting with bony erosion, which supports a less minacious portrayal of vertebral artery aneurysms.

  12. The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

    OpenAIRE

    Río Ospina, Luisa del; Soto de León, Sara; Camargo, Milena; Moreno Pérez, Darwin Andrés; Sánchez, Ricardo; Pérez Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso

    2015-01-01

    Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them...

  13. Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions

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    Vallejo-Ruiz Verónica

    2010-11-01

    Full Text Available Abstract Background Altered sialylation has been observed during oncogenic transformation. Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumor progression and metastases. In the cervical cancer high levels of sialic acid have been reported in the patients serum, and an increased of total sialic acid concentration has been reported for the cervical neoplasia and cervical cancer. This study investigates the changes in expression and distribution of α2,3-linked sialic acid and α2,6- linked sialic acid in low and high squamous intraepithelial lesions and in normal tissue. Methods Lectin histochemistry was used to examine the expression and distribution of sialic acid in different grades of cervical neoplasia. We applied Maackia amurensis lectin, which interacts with α2,3-linked sialic acid and Sambucus nigra lectin specific for α2,6-linked sialic acid. Results The histochemical analysis showed that α2,3-linked sialic acid and α2,6- linked sialic acid increased in intensity and distribution in concordance with the grade of squamous intraepithelial lesion (SIL. These results are in concordance with a previous study that reports increased RNAm levels of three sialyltransferases. Conclusions These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. These findings provide the basis for more detailed studies of the possible role of cell surface glycoconjugates bearing sialic acid in the cellular cervix transformation.

  14. Human Papillomavirus Types 52 and 58 Are Prevalent in Uterine Cervical Squamous Lesions from Japanese Women

    Directory of Open Access Journals (Sweden)

    Kazuhiro Takehara

    2011-01-01

    Full Text Available Objective. To estimate the prevalence and genotypes of high-risk human papillomavirus (HPV focusing HPV 16, 18, 52, and 58 in Japan. Methods. Liquid-base cytology specimens were collected from Japanese women (n=11022, aged 14–98. After classifying cytodiagnosis, specimens were analyzed for HPV DNA by the multiplex polymerase chain reaction method, where 1195 specimens were positive for cervical smear, except adenomatous lesions. Result. HPV genotypes were detected in 9.5% of NILM and 72.2% of ASC-US or more cervical lesions. In positive cervical smears, HPV genotypes were HPV 52 at 26.6%, HPV 16 at 25.2%, HPV 58 at 21.8%, and HPV 18 at 7.1%. Most patients infected with HPV 16 were between 20–29 years old, decreasing with age thereafter. As for HPV 52 and 58, although the detection rate was high in 30- to 39-year-olds, it also was significant in the 50s and 60s age groups. Conclusion. In Japan, as a cause of abnormal cervical cytology, HPV52 and 58 are detected frequently in addition to HPV 16. In older age groups, HPV 52 and 58 detection rates were higher than that observed for HPV 16. After widespread current HPV vaccination, we still must be aware of HPV 52 and 58 infections.

  15. Telomerase activity in high-grade cervical lesions is associated with allelic imbalance at 6Q14-22.

    NARCIS (Netherlands)

    Duin, van M.; Steenbergen, R.D.M.; Wilde, de J.; Helmerhorst, TJ; Verheijen, R.H.M.; Risse, E.K.J.; Meijer, C.J.L.M.; Snijders, P.J.F.

    2003-01-01

    Our study attempts to establish the relationship between telomerase activity and allelic imbalance (AI) on chromosomes 3p and 6 in high-risk HPV-containing cervical lesions. These chromosomes were implicated previously in telomerase regulation in HPV containing immortalized cells and cervical cancer

  16. Osteosíntesis cervical posterior con placa en lesiones cervicales

    OpenAIRE

    Sánchez Martín, Miguel María

    2002-01-01

    El propósito del presente estudio es analizar retrospectivamente los resultados a largo plazo de las lesiones de la columna cervical baja tratadas mediante estabilización posterior con placas atornilladas de Roy-Cmille a las masas laterales. Pacientes y métodos: Se estudian 15 pacientes, de los cuales sólo 14 completos con lesiones traumáticas (13) y 1 no traumática (1 paciente con inestabilidad sobre artrosis por doble Cloward) durante un periodo postoperatorio medio de 8 años (6-12). Salvo ...

  17. The Application Value of Colposcopy Combined with Cervical Cytology in Cervical Lesions%阴道镜检查结合宫颈细胞学在宫颈病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王少华

    2014-01-01

    目的:探讨阴道镜检查结合宫颈细胞学在宫颈病变诊治中的临床应用价值。方法随机选在该院接收的656例行阴道镜检查结合宫颈细胞学的妇女作为研究对象,对所有对象的资料进行回顾性分析,总结患者的治疗效果。结果单纯行阴道镜检查筛查3例,无漏诊,癌前期可疑1例。单纯行新柏氏液基细胞学检测检查筛选宫颈癌4例,漏诊1例。行阴道镜检查结合新柏氏液基细胞学检测符合宫颈癌病例5例,无漏诊。结论单纯性阴道镜检查或者新柏氏液基细胞学检测宫颈癌存在漏诊情况,而结合两种方法进行检查后无漏诊现象,即结合两种方法诊断早期宫颈癌有助于提高疾病检出率,值得在临床上推广和使用。%Objective To investigate the clinical application value of colposcopy combined with cervical cytology in the diagnosis and treatment of cervical lesions. Methods 656 cases of women underwent colposcopy combined with cervical cytology in our hos-pital from March, 2012 to March, 2013 were randomly selected as the subjects, and the data of them were analyzed retrospectively, the treatment effect was summarized. Results A simple colposcopy screening 3 cases, no missed diagnosis, pre-cancerous suspi-cious 1 case. Simple ThinPrep liquid-based cervical cytology screening examination in 4 cases, missed 1 case. Colposcopy com-bined with ThinPrep liquid-based cervical cytology in line 5 cases, no missed case. Conclusion Missed diagnosis exists in simple colposcopy or ThinPrep liquid-based cervical cytology for detecting cervical cancer, but that does not exist if combining the two methods for detecting cervical cancer, and the diagnosis of early cervical cancer by combining the two methods is conducive to im-proving the detection rate of the disease, which is worthy of clinical popularization and application.

  18. High grade squamous intraepithelial lesion in inmates from Ohio: cervical screening and biopsy follow-up

    Directory of Open Access Journals (Sweden)

    Rofagha Soraya

    2006-01-01

    Full Text Available Abstract Background Cervical carcinoma remains the second leading cause of cancer death in women worldwide and sexual behavior is regarded as the main contributing factor. We studied cervical cytology screening with surgical biopsy follow-up in women prisoners and compared the findings to those in the general population. Methods We reviewed 1024 conventional cervical smears, 73 cervical biopsies and 2 loop electrosurgical excision procedure (LEEP specimens referred to us from the Correctional Center in Columbus, Ohio during a 12-month period. The results were compared to 40,993 Pap smears from the general population for the same 12-month period. Results High grade squamous intraepithelial lesion (HGSIL was diagnosed in 1.3% of the cervical smears from the inmate population versus 0.6% in the general population (p < 0.01. The unsatisfactory rate was 1.6% compared to 0.3% in the general population (p < 0.01. Among the study population, follow-up tissue diagnosis was obtained in 24.3% of the abnormal cytology results (ASCUS, LGSIL, and HGSIL. Of the HGSIL Pap smears, 61.5% had a subsequent tissue diagnosis. Thirty-nine biopsies (52% of the all inmate biopsies and LEEP showed CIN II/III (cervical intraepithelial neoplasia II/III. Eight of these thirty-nine follow-up biopsies diagnosed as CIN II/III had a previous cervical cytology diagnosis of ASCUS. The average age for HGSIL was 30.5 years (S.D. = 5.7 and for low grade squamous intraepithelial lesion (LGSIL was 27.2 years (S.D. = 6.1. Conclusion A significantly higher prevalence of HGSIL cervical cytology and unsatisfactory smears was encountered in female inmates, with tissue follow-up performed in less than two thirds of the patients with HGSIL. These results are in keeping with data available in the literature suggesting that the inmate population is high-risk and may be subject to less screening and tissue follow-up than the general population. Clinicians should proceed with urgency to improve

  19. Cervical Liquid-based Cytology in Cervical Lesions the Pathological Diagnosis of Significance%宫颈液基薄层细胞学在宫颈病变病理诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    郑国文

    2013-01-01

      Objective To investigate the liquid-based cytology (TCT) value in pathological diagnosis of cervical lesions. Methods Retrospective analysis of 2009 January to 2012 June, 5795 patients with TCT cervical cytology, colposcopy biopsy positive cases, compared the two results. Results In 5499 cases of TCT were negative, 296 cases were positive, the positive rate was 5.11%, false negative in 15 cases, no false positive cases. 17 cases of biopsy tissue inflammation, 224 cases of CIN Ⅰ, 20 cases of CINⅡ, 30 cases of CINⅢ, CA 5 cases, LSIL and CIN Ⅰcoincidence rate was 80% (36/45), HSIL and CINⅡ/Ⅲcoincidence rate was 88.9% (24/27). Conclusion TCT examination has a little injury, high positive rate, strong repeatability, for married women can be used as the main method of screening for cervical cancer and precancerous lesions.%  目的探讨液基薄层细胞学(TCT)检查在宫颈病变病理诊断中的意义。方法回顾分析我院2009年1月至2012年6月5795例患者运用 TCT 进行宫颈脱落细胞学检查,阳性病例在阴道镜下活检,二者结果相比较分析。结果 TCT 检查阴性5499例,阳性296例,阳性率5.11%,假阴性15例,无假阳性病例。组织活检炎症17例,CIN Ⅰ224例,CIN Ⅱ20例,CIN Ⅲ30例,CA 5例,LSIL 与 CIN Ⅰ符合率80.0%(36/45),HSIL 与 CIN Ⅱ/Ⅲ符合率88.9%(24/27)。结论 TCT 检查具有损伤小,阳性检出率高,重复性强,对已婚妇女可作为宫颈癌及癌前病变筛查的主要方法。

  20. Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea

    Directory of Open Access Journals (Sweden)

    Carro-Campos Patricia

    2009-09-01

    Full Text Available Abstract Background The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most frequent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. Methods A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP. Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types. Results Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%. HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection. Conclusion The frequency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31 should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.

  1. Determination of CA-125 levels in the serum, cervical and vaginal secretions, and endometrium in Chinese women with precancerous disease or endometrial cancer

    OpenAIRE

    Shu-ming HE; Xing, Fuqi; Sui, Hong; Wu, Youming; Wang, Yongli; Wang, Dong; Chen, Guanghui; Kong, Zijing; ZHOU, SHU-FENG

    2011-01-01

    Summary Background Serum CA-125 has been used as a biomarker of gynecological tumors. In this study, we investigated the CA-125 levels in cervical and vaginal secretions from Chinese patients with endometrial polyps, hyperplasia and carcinoma in comparison with those in endometrium and serum. Material/Methods An electro-chemiluminescent immunoassay was utilized to determine the levels of CA-125 in 51 healthy Chinese women and 97 patients with polyps, hyperplasia or endometrial cancer. An immu...

  2. Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer.

    Directory of Open Access Journals (Sweden)

    Nina Milutin Gašperov

    Full Text Available Change in the host and/or human papillomavirus (HPV DNA methylation profile is probably one of the main factors responsible for the malignant progression of cervical lesions to cancer. To investigate those changes we studied 173 cervical samples with different grades of cervical lesion, from normal to cervical cancer. The methylation status of nine cellular gene promoters, CCNA1, CDH1, C13ORF18, DAPK1, HIC1, RARβ2, hTERT1, hTERT2 and TWIST1, was investigated by Methylation Specific Polymerase Chain Reaction (MSP. The methylation of HPV18 L1-gene was also investigated by MSP, while the methylated cytosines within four regions, L1, 5'LCR, enhancer, and promoter of the HPV16 genome covering 19 CpG sites were evaluated by bisulfite sequencing. Statistically significant methylation biomarkers distinguishing between cervical precursor lesions from normal cervix were primarily C13ORF18 and secondly CCNA1, and those distinguishing cervical cancer from normal or cervical precursor lesions were CCNA1, C13ORF18, hTERT1, hTERT2 and TWIST1. In addition, the methylation analysis of individual CpG sites of the HPV16 genome in different sample groups, notably the 7455 and 7694 sites, proved to be more important than the overall methylation frequency. The majority of HPV18 positive samples contained both methylated and unmethylated L1 gene, and samples with L1-gene methylated forms alone had better prognosis when correlated with the host cell gene promoters' methylation profiles. In conclusion, both cellular and viral methylation biomarkers should be used for monitoring cervical lesion progression to prevent invasive cervical cancer.

  3. One-year clinical evaluation of compomer restorations in cervical lesions of different aetiology

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    Premović Milica

    2012-01-01

    Full Text Available Introduction. The aim of this one-year prospective clinical study was to evaluate the treatment results of compomer restorations (Dyract® eXtra/ Dentsply/De Trey, Konstanz, Germany with a single step self-etching dental adhesive (Xeno® III Dentsply/De Trey, Konstanz, Germany used for restoring class V lesions (non-carious and primary carious cervical lesions. Material and Methods. A total number of 62 class V restorations (n = 62 were placed by one dentist in 30 patients on incisors, canines and premolars. The fillings were placed due to different indications: non-carious cervical defects (n = 32 and primary carious lesions (n = 30. The restorations were evaluated by a single-blind design, according to the Modified United States Public Health Service system 6 and 12 months following the placement. The following were evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. The statistical analysis compared the ratings of each criteria between materials using the Pearson chi-square or Fisher’s exact test at a level of significance of 5% (p<0.05. Results. Two restorations of the non-carious lesion group were lost after 6 months, and after 12 months one restoration was lost in the group of primary carious lesions. There were no statistically significant differences between restorations for all evaluated criteria in both groups. Conclusion. The compomer restorations in combination with a single step self-etching dental adhesive showed acceptable clinical performance in Class V lesions after one year of clinical service.

  4. 宫颈薄层液基细胞学检查联合高危型HPV检测在宫颈病变筛查中的临床应用%CLINICAL APPLICATION OF CERVICAL THIN PREP CYTOLOGIC-TEST AND HIGH-RISK HPV TYPE TESTING IN SCREENING OF CERVICAL LESIONS

    Institute of Scientific and Technical Information of China (English)

    朱华洁

    2012-01-01

    目的 研究宫颈薄层液基细胞学检查(thin prep cytologic test,TCT)联合高危型人乳头瘤病毒(human papilloma virus,HPV)检测在子宫颈病变筛查中的诊断价值.方法对2010年1-12月行宫颈病变筛查的患者680例,进行TCT和高危型HPV检测,同时行阴道镜下组织活检.结果 680例患者中宫颈TCT检查结果异常180例,对宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)诊断的敏感性为85.57%,特异性为97.12%,漏诊率为4.12%.TCT与HPV联合检查,诊断敏感性为89.69%,特异性为85.80%,漏诊率1.62%.HPV感染率为26.91%,随着细胞学和组织学诊断级别的升高,HPV感染率不断上升.结论 TCT与高危型HPV联合检测是筛查宫颈癌及癌前病变的有效方法,可防止漏诊,有利于提高宫颈上皮内瘤变的诊断率.%Objective To explore the diagnostic value of cervical thin prep cytologic test ( TCT ) combined with high - risk human papilloma virus ( HPV ) detection in the screening of cervical lesions. Methods A total of 680 patients for cervical lesions screeing underwent TCT and high - risk HPV type detection in gynecology outpatient from January 2010 to December 2010, meanwhile, biopsy was performed under colposcopy. Results Among 680 cases,the result of cervical TCT was abnormal in 180 cases. The sensitivity, specificity and the rate of missed diagnosis of TCT for the diagnosis of cervical intraepithelial neoplasia ( CIN ) was 85.57% , 97. 12% and 4. 12% , respectively, while thoses of combination of TCT and HPV was 89. 69% ,85. 80% and 1. 62%. The infection rate of HPV was 26. 91%. With the increase of cytological and histological grading, the infection rate of HPV increased. Conclusion TCT combined with high risk HPV detection is an effective method in screening cervical cancer and precancerous lesions. It helps to prevent missed diagnosis and improve the diagnosis rate of CIN.

  5. Cervical intraepithelial lesions in females attending Women′s Health Clinics in Alexandria, Egypt

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    Mona Abdel-Hadi

    2015-01-01

    Full Text Available Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to have a representative sample for women living in Alexandria. All women included did not have any cervical disorder related complaints. Conventional Pap smears were obtained and diagnosed using the Bethesda system. Women with abnormal Pap smears were managed according to the 2006 consensus guidelines within the available facilities. Persistent abnormal cytological results were referred for colposcopic biopsy. Histological results were grouped into: Reactive changes, CIN 1, CIN 2/CIN 3 and adenocarcinoma in-situ (AIS. Results: Out of the 6173 smears included in the study 6072 (98.36% were normal and only 101 (1.63% were abnormal. After colposcopic biopsies, 0.08% had CIN 1, 0.03% had CIN 2, 3 and 0.01% had AIS. Conclusion: We concluded that cervical cancer screening programs, although life-saving for a number of women, are not a sufficiently high priority in our community. Money for national health screening programs should preferably be directed more towards recruiting women for breast cancer screening, since breast cancer accounts for about 33% of all female cancers in Egypt ranking number one, while cervical cancer ranks number 13.

  6. Bone marrow mesenchymal stem cell transplantation for gastric precancerous lesions in a rat model%骨髓间充质干细胞移植对大鼠胃癌前病变模型的修复作用

    Institute of Scientific and Technical Information of China (English)

    刘洪锋; 李潞

    2016-01-01

    背景:胃癌前病变是多种因素共同参与的长期发展过程,骨髓间充质干细胞有修复组织损伤的作用。目的:探讨骨髓间充质干细胞移植对大鼠胃癌前病变的影响。  方法:将36只Wistar大鼠随机分为对照组、模型组和移植组。模型组和移植组建立大鼠胃癌前病变模型,移植组大鼠经尾静脉注射1 mL CM-dil标记的骨髓间充质干细胞(3×106个细胞),每周注射1次,一共3次,模型组和对照组同样方法经尾静脉注入等量的生理盐水。末次注射后1周处死大鼠,观察大鼠胃组织病理变化,检测胃黏膜中血管内皮生长因子的表达以及血清中细胞因子含量。  结果与结论:①移植组大鼠胃黏膜损伤较模型组轻,移植组血管内皮生长因子表达明显高于模型组和对照组(P<0.05);②模型组和移植组血清白细胞介素17和γ-干扰素水平高于对照组(P<0.05),移植组血清白细胞介素17和γ-干扰素水平低于模型组(P<0.05);③结果表明,骨髓间充质干细胞通过增加胃黏膜病变部位血管内皮生长因子的表达,降低血清白细胞介素17和γ-干扰素水平,从而提高血管通透性,减轻炎症反应,阻断或者缓解癌前病变的发生。%BACKGROUND:Precancerous lesions are a long-term development process in which many factors are involved. Bone marrow mesenchymal stem cel s can repair tissue injury. OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cel transplantation on gastric precancerous lesions in the rats. METHODS:Thirty-six Wistar rats were randomly divided into control group, model group and transplantation group. Animal models of gastric precancerous lesions were established in the model and transplantation groups. Rats in the transplantation group were given 1 mL of CM-dil-labeled bone marrow mesenchymal stem cel s (3×106 cel s) via the tail vein, once a week, total y three times. Rats in the

  7. MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Cervical cancer is the main cause of death inwomen.The influence of HPV plays an i mportantrole incervial cancer.It has been provedthat humanpapillomavirus(HPV)infectionis ani mportant fac-tor in cervical carcinogenesis.Multiple HPVinfec-tion was associated less frequently with cervical car-cinoma and with precancerous lesions compared withnor mal cytology[1].The activation of oncogene,in-activition of tumor suppressor gene and instabilityof genome are also majority reason.We establisheda cell line of human...

  8. Application of thinprep cytology test and cervical smear combined with colposcope in screening of cervical lesions%液基细胞学、宫颈刮片联合阴道镜在宫颈病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    江明珠

    2015-01-01

    目的 探讨液基细胞学(TCT)、宫颈刮片联合阴道镜在宫颈病变筛查中的应用价值. 方法 选取2014年1月~2015年4月来我所妇科门诊行TCT筛查的1726例妇女(门诊组)和在乡镇行宫颈刮片细胞学筛查的26 021例妇女(乡镇组)作为研究对象,两组均行TBS分级诊断,将细胞学阳性者进一步行阴道镜及镜下活组织病理学检查,分析两组不同取材方法的细胞学筛查结果,并观察细胞学阳性者的阴道镜和病理组织学检查结果. 结果 两组细胞学检出阳性率差异有统计学意义(X2=86.56,P0.05). 以病理组织学诊断为金标准,宫颈癌前病变和宫颈癌(CIN+SCC)两者合计,阴道镜诊断符合率为90.14%(128/142), 细胞学诊断符合率为70.73%(87/123). 结论 TCT筛查法优于传统刮片细胞学筛查法,联合应用阴道镜及镜下活检组织病理学检查,可明显提高宫颈癌前病变及宫颈癌的检出.%Objective To discuss the application value of thinprep cytology test(TCT) and cervical smear combined with colposcope in screening of cervical lesions. Methods 1726 cases of female patients (outpatient group) visited outpatient clinic in our hospital from January 2014 to April 2015 and 26 021 cases of rural women (rural group) receiving cervical cancer screening were selected as research object.Two groups were diagnosed by TBS classification,and those with pos-itive cytologic results were further examined by colposcope and histopathological examination.The cytologic screening results in two groups were analyzed by different methods, the colposcope and histopathologic results of patients with positive cytologic results were observed. Results There was significant difference in positive rate of cytologic results be-tween two groups (X2=86.56,P0.05).The gold standard was diagnosed by histopathology.The total of cervical precancerous lesions and cervical cancer (CIN+SCC),colposcope diagnosis coincidence rate was 90.14%(128/142),and cytology

  9. Diagnostic analysis of endoscopic Lugol liquid staining for early esophageal cancer and precancerous lesions%内镜下卢戈氏液染色对早期食管癌及癌前病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李琪毅; 姜仕柱; 李素玉

    2013-01-01

    目的 探讨卢戈氏液染色对早期食管癌及癌前病变的诊断价值.方法 对本院内镜室2011年11月-2013年4月的187例患者的食管表浅病灶进行卢戈氏液染色,并行病理学检查,将病理结果与染色结果进行对比.结果 187例患者中经病理确诊为食管鳞癌41例,食管重度不典型增生9例,食管中度不典型增生33例,食管轻度不典型增生15例,慢性食管炎58例,鳞状上皮增生31例.卢戈氏液染色后食管癌及癌前病变主要表现为卢戈氏液不染和淡染,卢戈氏液淡染和不染诊断食管癌与癌前病变的准确性为62.82%(98/156);食管癌和食管重度不典型增生主要表现为卢戈氏液不染,其准确性高达95.6%(45/48).结论 内镜下卢戈氏液染色对鉴别食管癌、癌前病灶与炎性病灶等具有十分重要的临床价值.%Objective To investigate the clinical and pathological diagnosis of Lugol solution to early esophageal cancer and precancerous lesions.Methods One hundred and eighty-seven patients with suspicious lesions of esophageal endoscopy in our hospital from Nov.2011 to Apr.2013 were chosen as study objects and were dyed with Lugol of the liquid and were given pathological examination.Pathology results and the results of dye were compared.Results Forty-one out of the 187 cases were found with esophageal squalors carcinoma,9 with severe esophageal dysplasia,33 with moderate esophageal dysplasia,15 with esophageal mild dysplasia,58 with chronic esophagi,31 with squalors cell hyperplasia.After Lugol solution dyeing,the signifying of esophageal cancer and precancerous lesions was not pollution or light pollution,and the accuracy was 62.82%.Lugol non-staining liquid esophagus and esophageal cancer diagnosis of severe dysplasia was as high as 93.75% in accuracy.Conclusion Endoscopic Lugol solution staining confined with superficial esophageal biopsy of the lesion have important value in diagnosing superficial esophageal lesion.

  10. 液基细胞学及HPV分型检测在宫颈病变筛查中的应用%Thinprep Cytology Test and HPV Genotyping in Screening of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    焦凤娟; 韩洁

    2013-01-01

    Objective: To investigate the clinical significance of thinprep cytology test (TCT) and HPV genotyping in cervical le-sions. Methods: TCT and HPV genotyping were done for 390 cases of cervical lesions, and did the biopsy for who was ≥ASCUS. Results: In the 390 cases, the positive rates of TCT and HPV were 26.41% and 28.72%; the rate of high-risk type of HPV in 103 cas-es of TCT-positive patients was 63.11%, and the biopsy showed that there were inflammation 46 cases, CIN I 24 cases, CIN II 12 cases, CIN III 18 cases and cervical cancer 3 cases. Conclusion: TCT and HPV genotyping can improve the detection rate of cervical cancer and precancerous lesions, it has important significance in early diagnosis and prevention for cervical cancer.%  目的:探讨液基细胞学(TCT)联合HPV 分型检测在宫颈病变筛查中的临床意义.方法:对390例宫颈病变患者行TCT 检查和 HPV 检测,对≥ASCUS 患者行组织活检.结果:390例患者中 TCT 和 HPV 阳性率分别为26.41%、28.72%;103例TCT阳性患者中高危型HPV阳性率为63.11%,组织活检结果为炎症46例、CIN I 24例、CIN II 12例、CIN III 18例和宫颈癌3例.结论:TCT联合HPV检测可提高对宫颈癌及癌前病变的检出率,对宫颈癌的早期诊断和防治有重要临床意义.

  11. 不同检测方法在宫颈病变和早期宫颈癌筛查中的价值%The Screening Value of Different Detection Methods for Cervical Lesions and Early Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张雯; 安红梅; 马丽莎; 邓继红

    2011-01-01

    of invasive carcinoma). The highest sensitivity and Youden index ofHC-E test to diagnose the cervical le-sbnsmore servere than the high grade squanous intraepithelial lesbr( HSIL) were92.31% and0.81 respectively TCT, VIA, cot poscopy and Pap snearhad amoderate degree of sensitivity to diagnose cervical lesions more servere than the HSIL HPV-DNA+ TCT diagnosis was with the highest sensitivity, specificity and Youden index to diagnose cervical lesions more servere than the HSIL Con-clisim : TCT combined with HPV test is the optmal choice for the screening of early cervical lesions and cervical cancer the second recommended program is Pap snear+ HPV test; The general program consists ofHPV test and colposcopy, and the fundamental program vs comprised ofPap smear+ VIA. Complementarymulti-technobgy screening can find a large numberof precancerous lesions and cervical cancer Medical staff should select the appropriate screening program according to the patients" actual situation

  12. Comparative evaluation of genetic assays to identify oral pre-cancerous fields

    NARCIS (Netherlands)

    J.F. Bremmer; B.J. Braakhuis; A. Brink; M.A. Broeckaert; J.A.M. Beliën; G.A. Meijer; D.J. Kuik; C.R. Leemans; E. Bloemena; I. van der Waal; R.H. Brakenhoff

    2008-01-01

    Background: Oral squamous cell carcinomas often develop in a pre-cancerous field, defined as mucosal epithelium with cancer-related genetic alterations, and which may appear as a clinically visible lesion. The test characteristics of three genetic assays that were developed to detect pre-cancerous f

  13. Clinical Value Analysis of Endoscopic Indigo Carmine-Methylene Blue Double Staining in Diagnosis of Early Gastric Cancer and Precancerous Lesions%内镜下靛胭脂-美兰双重染色法诊断早期胃癌及癌前病变的临床价值分析

    Institute of Scientific and Technical Information of China (English)

    靳斌; 张永红; 赵梦云; 朱玉侠; 张雪丽; 李蓉; 路伟; 杨芸; 孔祥云; 赵明星; 韦斌

    2015-01-01

    Objective: To investigate clinical value of the endoscopic indigo carmine-methylene blue double staining in diagnosis of early gastric cancer and precancerous lesions.Methods:The patients to seek treatment with routine endoscopy, those of 112 suspicious lesions were randomly divided into two groups, the observation group were taken biopsy after endoscopic indigo carmine-methylene blue staining.The control group were routine biopsy.Results:16 cases of early gastric cancer and 27 cases of precancerous lesions were detected in the observation group (57 cases).9 cases of early gastric cancer and 19 cases of precancerous lesions were detected in the control group (57 cases).The early gastric cancer and precancerous lesion positive rate of the observation group and the control group was 43/57 (75.4%), and 28/55 (50.9%), respectively.The difference was statistically significant (P<0.01).There are obvious staining demarcation zone 28/57 (49.1%) of gastric cancer and precancerous lesion detection rate was significantly higher than those without clear demarcation zone staining 15/57 (26.3%) (P<0.05).Conclusion:Endoscopic indigo carmine methylene blue double staining can significantly improve the detection rate of early gastric cancer and precancerous lesion,that has the certain instruction significance or the early diagnosis of gastric cancer and precancerous lesions.%目的:探讨内镜下靛胭脂-美兰双重染色法对诊断早期胃癌及癌前病变的临床应用价值.方法:来就诊患者行常规内镜检查,将有可疑病灶患者112例随机分为两组,观察组行内镜下靛胭脂-美蓝染色后活检,对照组采用后常规活检.结果:观察组(57例)分别检出早期胃癌16例、癌前病变27例.对照组(55例)分别检出早期胃癌9例、癌前病变19例,观察组与对照组早期胃癌和癌前病变检出率分别为43/57(75.4%)和28/55(50.9%),且差异有统计学意义(P<0.01).有明显染色分界区28/57(49.1%)的胃癌和癌前病变

  14. 口腔HPV携带者子宫颈病变的发病风险分析%Risk of cervical cancer and precancerous diseases in the oral HPV carriers

    Institute of Scientific and Technical Information of China (English)

    何以丰; 张梅莹; 冯令达; 殷怡华; 张蓉; 狄文

    2013-01-01

    .2-32.1),respectively.The oral sex and multi-sexual partner were two major risk factors for the oral and cervical HPV co-infection,HPV-related cervical cancer and precancerous diseases according to behavioral analysis.Conclusions There are complicated transmission pathways between oral and cervicalHPV.Oral HPV carriers should be intensively followed up and their oral HPV infection and HPV-related cervical diseases should be treated together.

  15. Human papilloma virus genotypes in women from Nayarit, Mexico, with squamous intraepithelial lesions and cervical cancer

    Science.gov (United States)

    Ortega-Cervantes, Laura; Aguilar-Lemarroy, Adriana; Rojas-García, Aurora Elizabeth; Barrón-Vivanco, Briscia Socorro; Vallejo-Ruiz, Verónica; León, David Cantú-De; Hernández, Yael Yvette Bernal; Jáuregui-Martínez, Armando; Medina-Díaz, Irma Martha

    2016-01-01

    Objective In Mexico cervical cancer (CC) is the most common cause of death from neoplasia in women. Study aimed to analyze the current distribution of Human papillomavirus (HPV) types in women from Nayarit, Mexico, with Squamous intraepithelial lesions (SIL) and Cervical cancer (CC). Methodology Between January 2011 and July 2013, cervical samples were collected from female residents of the Mexican state of Nayarit and were analyzed by means of a LINEAR ARRAY® HPV genotyping test. Data analyses were performed using Stata ver. 8.0 statistical software. Results Of the samples analyzed, 91.2%, HPV DNA was detected. Of these positive samples, 82% were High-risk (HR) viral types. The most prevalent HPV genotypes identified were 16, 58, 31, 18, and 70. Forty two percent of participants had a single infection, while 23 and 26% of participants were infected with two or more HPV genotypes, respectively. HPV 16 was the most prevalent genotype identified and was frequently present as a co-infection with HPV types 18, 51, 52, 59, 66, or 70. Conclusion Women <20 years of age were most often infected with HPV, and the HPV Quadrivalent vaccine (types 16, 18, 6, and 11), currently available in Mexico, no confers protection against a subset of the HPV genotypes identified in the present study (58, 31, 70, and 35). Thus, it is important evaluate the geographical distribution of specific HPV genotypes in all health of center across Mexico in order to implement a successful vaccination program and to diagnose CC in its early stages. PMID:27610056

  16. 喉癌前病变DNA含量测定及细胞周期分析的意义%The clinical significance of DNA content and cell-cycle analysis in laryngeal precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    周剑勇; 梁素霞

    2001-01-01

    Objective To investigate the value of the DNA content and cell-cycle analysis in diagnosing laryngeal precancerous lesion and laryngeal carcinoma. Methods 62 cases including 4 laryngeal normal epithlium, 5 laryngeal keratosis, 8 atypical hyperplaisa cell, 9 papilloma and 36 laryngeal carcinoma were examined with flow cytometry (FCM). Results In continuous process of normal cell to cancerous cell, the DNA index, cell-cycle S-fractions and proliferative index gradually increased, with the appearance of aneuploid cells. Compared with other groups, laryngeal cerainoma was characterized by a high percentage of aneuploid cells (P<0.005). Conclusion The DNA quantitive analysis with FCM is helpful for the early dignosis of laryngeal carcinoma.%目的 探讨DNA含量和细胞周期分析在喉癌前病变诊断中的价值。方法 用流式细胞仪测定正常喉上皮、角化病、不典型增生、乳头状瘤及喉鳞癌共62例患者的DNA含量及细胞周期。结果 从正常喉细胞到癌细胞的变化过程中,DNA含量、S期细胞比例及增殖活性逐渐增加(P<0.01),且出现非整倍体细胞。喉癌细胞非整倍体率达91.7%,超出了其他各组(P<0.005)。结论 流式细胞DNA定量分析有助于喉癌的早期诊断。

  17. Motor Deficit After Cervical Surgery, Parsonage-Turner Syndrome or Root Lesion

    Directory of Open Access Journals (Sweden)

    Ayse Sarifakioglu

    2013-06-01

    Full Text Available A 60 years old male patient was admitted to our outpatient clinic with the complaint of severe pain in right arm after cervical stenosis surgery which had been decreased with the formation of progressive motor weakness. Although clinical and physical evaluation was compatable with Parsonage-Turner syndrome (PTS, C5 root involvement has been detected. The patient was given exercise therapy with TENS for analgesia. The patient%u2019s motor weakness progressed and he had difficulties with daily living activities. With the review of literature, we are presenting a case, whose diagnosis was difficult in terms of differentiating both PTS and root lesion which could only be diagnosed by electroneuromyography.

  18. Two-year clinical evaluation of three adhesive systems in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Evrim Eliguzeloglu Dalkilic

    2012-04-01

    Full Text Available OBJECTIVES: Adhesive systems are continuously being introduced to Dentistry, unfortunately often without sufficient clinical validation. The aim of this study was to evaluate the clinical performance of cervical restorations done with three different adhesive systems. MATERIAL AND METHODS: 158 non-carious cervical lesions of 23 patients were restored with a nanofilled composite resin (Filtek Supreme, 3M/ESPE combined with Single Bond (3M/ESPE, group SI, Clearfil SE (Kuraray Medical Inc., group CL and Xeno III (De Trey Dentsply, group XE. In groups SI-B, CL-B and XE-B, the outer surface of the sclerotic dentin was removed by roughening with a diamond bur before application of the respective adhesive systems. In groups CL-BP and XE-BP, after removal of the outer surface of the sclerotic dentin with the bur, the remaining dentin was etched with 37% phosphoric acid and the self-etch adhesive systems Clearfil SE and Xeno III were applied, respectively. Lesions were evaluated at baseline, and restorations after 3 months, 1 year and 2 years using modified USPHS criteria. RESULTS: After 2 years, no significant difference was found between the retention rates of the groups (p >0.05. Although groups CL and SI showed significantly better marginal adaptation than group XE (p0.05. After 2 years no significant difference was observed among the marginal staining results of all groups (p>0.05. CONCLUSION: Although all adhesive systems showed similar retention rates, Clearfil SE and Single Bond showed better marginal adaptation than Xeno III after 2 years of follow-up.

  19. Study on detection and treatment of cervical lesions in 10 212 women in Yingcheng%应城市10212例妇女宫颈病变检测及治疗的研究

    Institute of Scientific and Technical Information of China (English)

    刘永桥; 李艳; 杨丽

    2012-01-01

    Objective; To detect, diagnose, and evaluate cervical lesions, especially the degree and state of cervical precancerous lesion by liquid - based thin layer cytological test ( TCT) combined with biopsy, provide an accurate reference for treating cervical lesions reasonably in clinic, observe the curative effect. Methods; A total of 10 212 women were detected by TCT for successive three years, including 457 female workers from the hospital. Cytological diagnosis was made according to TBS criteria. Partial women with positive TCT results received cervical biopsy. Results; Among 10 212 women, 391 women were found with positive cytological results (above ASC - US) , the positive rate was 3. 83% , including 296 women with ASC - US (2. 90% ) , 85 women with ASC - H ( 0. 84% ) , 5 women with LSIU (0. 05% ) , 3 women with HSIL (0. 03% ) , one woman with SCC (0. 01% ) , and one woman with AGC (0. 01% ) . The positive rate of cytologieal screening in 457 female workers in the hospital for successive three years was 3.42% , 0. 88% , and 0.00% , respectively. Conclusion: TCT has distinct advantages for cervical lesions screening and curative effect observation after gynecological treatment , TCT combined with cervical biopsy and treatment can reduce the occurrence and development of cervical lesions, which has important clinical application value.%目的:采用液基薄层细胞制片技术(TCT)结合组织活检术检测、诊断和评价宫颈病变,特别是癌前病变的程度及所处的状态,为临床合理治疗宫颈病变提供准确依据,观察治疗后的效果.方法:运用TCT连续3年检测了10 212例妇女,包括457例应城市人民医院女职工.细胞学诊断按TBS标准.对TCT部分阳性者进行宫颈活检.结果:10 212例妇女中,细胞学阳性(ASC-UC以上)391例,阳性率为3.83%,其中ASC-UC 296例(2.90%)、ASC-H 85例(0.84%)、LSIL 5例(0.05%)、HSIL 3例(0.03%)、SCC 1例(0.01%)、AGC 1例(0.01%).应城市人民医院457

  20. Role of cytokine gene (interferon-γ, transforming growth factor-β1, tumor necrosis factor-α, interleukin-6, and interleukin-10) polymorphisms in the risk of oral precancerous lesions in Taiwanese.

    Science.gov (United States)

    Hsu, Han-Jen; Yang, Yi-Hsin; Shieh, Tien-Yu; Chen, Chung-Ho; Kao, Yu-Hsun; Yang, Chia-Fu; Ko, Edward Cheng-Chuan

    2014-11-01

    Oral squamous cell carcinoma can be preceded by some benign oral lesions with malignant potential, including leukoplakia, erythroplakia, oral lichen planus, and oral submucous fibrosis. There are different degrees of inflammatory cells infiltration in histopathology. Inflammatory cytokines may play a pathogenic role in the development of oral precancerous lesions (OPCLs). Genetic polymorphisms of cytokine-encoding genes are known to predispose to malignant disease. We hypothesized that the risk of OPCLs might be associated with cytokine gene polymorphisms of interferon (IFN)-γ, transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10. In the present study, 42 OPCL patients and 128 controls were analyzed for eight polymorphisms in five different cytokine genes [IFN-γ (+874 T/A), TGF-β1 (codons 10 T/C and 25 G/C), TNF-α (-308 G/A), IL-6 (-174 G/C), and IL-10 (-1082 A/G, -819 T/C, and -592 A/C)]. Cytokine genotyping was determined by the polymerase chain reaction sequence-specific primer technique using commercial primers. Allele and genotype data were analyzed for significance of differences between cases and controls using the Chi-square (χ(2)) test. Two-sided p < 0.05 were considered to be statistically significant. A series of multivariate logistic regression models, adjusted for age, sex, betel quid chewing, alcohol consumption, and smoking, was constructed in order to access the contribution of homozygous or heterozygous variant genotypes of polymorphisms. The TNF-α (-308) polymorphism was significantly associated with OPCLs. There were significant differences in the distribution of AA, GA, and GG genotypes between OPCL patients and controls (p = 0.0004). Patients with the AA or GA genotype had a 3.63-fold increased risk of OPCLs. The TGF-β1 (codon 10 and 25) polymorphism was also significantly associated with OPCLs (p < 0.001). The IL-6 polymorphism was significantly associated with OPCLs. There are significant

  1. Effects of glutamine and arginine on the gastric precancerous lesions in rats%精氨酸和谷氨酰胺对胃黏膜癌前病变影响的实验研究

    Institute of Scientific and Technical Information of China (English)

    江涛; 舒晓亮; 徐涵; 曾英

    2012-01-01

    Objective: To investigate the effect of glutamine and arginine on gastric precancerous in Sprague-Dawley rats. Methods: One-hundred rats were randomly divided into test A group (n = 20 ) , test B group ( n = 20 ) , test C group ( n = 20 ) , control group ( n = 20 ) , normal group ( n = 20). N-methyl-N-nitro-N-nitrosoguanidine(MNNG,100μg/mL) was used feeding rats to make animal model of gastric precancerous lesions. All rats were fed with Nutrison as base enteral nutrition. The rats in testA, testB and testC groups were added with glutamine +arginine, arginine and glutamine in Nutrison,respectively. After 8 weeks,all rats were killed. Immune function were measured. Expression of oncogenes were also determined. Histopathological changes of gastric mucosa were observed. Results: The levels of T-lymphocyte subsets CD4,the ratio of CD4/CD8, immunoglobulin A(IgA)and immunoglobulin M(IgM) were higher, but the levels of tumor necrosis factor(TNF-α) and interleukin-6(IL-6) were lower in test A, test B, test C groups than those in the control group. Compared with the control group, the expression of bcl-2, c-myc and the incidence of mild to severe metaplasia, dysplasia were significantly lower in test A, test B, test C groups . The expression of bcl-2,c-myc and the incidence of mild to severe metaplasia, dysplasia were lower in test A than those in test B, test C groups. There were not statistic difference among test B, test C groups. Compared with normal group,the levels of IgA,IgM, IgG,CD4 and the ratio of CD4/CD8 were lower, the levels of TNF-α,IL-6 and CD8 were significantly highter in control groups. The expression of bcl-2, c-myc and the incidence of mild to severe metaplasia, dysplasia were significantly higher in control group than those in normal group. Conclusion: Glutamine and arginine can regulate immune function and cell proliferation and effectively intervene in the development of gastric precancerous lesions in rats.%目的:研究精氨酸(Arg)和谷氨酰

  2. Methods of diagnosis in cervical neoplasia

    Directory of Open Access Journals (Sweden)

    Mariana Bratu

    2010-06-01

    Full Text Available The early screening of a precancerous lesion that can develop spontaneously into a cancerous lesion thatis first non-invasive and then invasive. Materials and methods. The study group was made of 1485 patients who were hospitalized between 2001 and 2009 in the Section of Obstetrics and Gynecology of ?Sf. Apostol Andrei? Emergency Hospital in Gala?i. The patients filled in a questionnaire on a sheet of paper in a freely consented way and they were diagnosed with cervical cancer by a cytologic and colposcopic examination, biopsy and other complementary examinations. Results and discussions. It is obvious that, even if cervical carcinoma benefits now from a remarkable methodology of detecting the early forms and even the precursor lesions, in our country this lesion has an importantfrequency and even represents a public health issue with important social-economic implications; in most cases it affects people in full physical and procreative activity; it implies a laborious therapy, long time hospitalization and high costs. The risk of getting infected with HPV(human papilloma virus is at least 50% for the sexually active people for the rest of their lives. Although most infections eliminate with the help of our own immunity, the people who are infected are not aware of HPV presence and they can spread the virus. When our own immune system cannot eliminate the infection, thepersistence of the viral oncogenic strains from the cervical mucosa can lead to the appearance of precancerous lesions.The cytological examination is a simple and fast method that is also cheap and reliable being considered the mosteffective technique for preventing and detecting the precancerous conditions of the cervix, which, treated correctly, canoffer a primary prevention of cervical cancer. The diagnostic certainty was accomplished exclusively by histopathologyof the material obtained during biopsy. Conclusions. We consider that the patients with cervical cancer, no matter

  3. Human papillomavirus types distribution in eastern Sicilian females with cervical lesions. A correlation with colposcopic and histological findings.

    Science.gov (United States)

    Le Donne, Maria; Giuffrè, Giuseppe; Caruso, Carmela; Nicotina, Piero Antonio; Alibrandi, Angela; Scalisi, Rosalba; Simone, Angela; Chiofalo, Benito; Triolo, Onofrio

    2013-07-01

    To determine human papillomavirus (HPV) types distribution in cervical lesions in a Southern Italian female population in Messina and their relationship between HPV type and grade of colposcopic and histopathological abnormality, a total of 253 women aged 17-68 years, with previous cytological abnormalities, were included in this study. HPV-DNA testing, colposcopy and biopsy were performed. For each sample, cervical cells were collected by centrifugation and DNA was extracted, followed by a PCR-based HPV-DNA assay and reverse dot blot genotyping. HPV-16 was found the most common type (46.6 %) followed by HPV-31 (26.9 %), -6 (18.6 %), -58 (8.8 %), -18 (6.7 %), -66 (5.7 %), -52 and -53 (4.7 %). Out of 62 women with abnormal transformation zone (ATZ) area compatible with squamous intraepithelial lesion (SIL) or cervical cancer (CC), 64.5 % was found high risk (HR) HPV-positive. Moreover the severity of the colposcopic diagnosis was positively correlated with the higher HPV oncogenicity risk (HPV-16 P = 0.023; and HPV-53 P = 0.047). The HPV-16 was found the most prevalent type within each histological category: 66.7 %, 31.2 %, 44 % and 37.2 % of CC, high grade (H)SIL, low grade (L)SIL and chronic cervicitis respectively; followed by HPV-31 present in 25 %, 8 %, and 13.3 % of HSIL, LSIL and chronic cervicitis respectively. A higher HPV incidence than the rest of Italy was found, in agreement with that detected by other authors for the South of the country. These data provide further information about the types prevalence in women with cervical lesions living in Eastern Sicily, suggesting the introduction of new targeted vaccines against a wider spectrum of HPV.

  4. Aberrant DNA methylation in cervical carcinogenesis

    Institute of Scientific and Technical Information of China (English)

    Hui-Juan Yang

    2013-01-01

    Persistent infection with high-risk types of human papillomavirus(HPV) is known to cause cervical cancer; however,additional genetic and epigenetic alterations are required for progression from precancerous disease to invasive cancer.DNA methylation is an early and frequent molecular alteration in cervical carcinogenesis.In this review,we summarize DNA methylation within the HPV genome and human genome and identify its clinical implications.Methylation of the HPV long control region (LCR) and L1 gene is common during cervical carcinogenesis and increases with the severity of the cervical neoplasm.The L1 gene of HPV16 and HPV18 is consistently hypermethylated in invasive cervical cancers and can potentially be used as a clinical marker of cancer progression.Moreover,promoters of tumor suppressor genes (TSGs) involved in many cellular pathways are methylated in cervical precursors and invasive cancers.Some are associated with squamous cell carcinomas,and others are associated with adenocarcinomas.Identification of methylated TSGs in Pap smear could be an adjuvant test in cervical cancer screening for triage of women with high-risk HPV,atypical squamous cells of undetermined significance,or low grade squamous intraepithelial lesion (LSIL).However,consistent panels must be validated for this approach to be translated to the clinic.Furthermore,reversion of methylated TSGs using demethylating drugs may be an alternative anticancer treatment,but demethylating drugs without toxic carcinogenic and mutagenic properties must be identified and validated.

  5. HC2-HPV-DNA 检测在宫颈癌病变中的临床分析%Clinical Analysis of HC2-HPV-DNA Testing in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    季冰; 何浪驰; 郭芝亮; 施全; 黄子健; 黄峥; 黄柳

    2015-01-01

    Objective To observe hybrid capture 2 high-risk human papilloma virus DNA(HC2-HPV-DNA) in cervical lesion screening and accuracy .Methods 110 cases of patients with suspected symptoms of cervical lesion were selected as the research object,and respectively received liquid-based thinlayer cytology test(LCT)inspection,HC2-HPV-DNA testing and cervi-cal tissues pathology biopsy .Positive rate,sensitivity,specificity,and predictive value of LCT,HC2-HPV-DNA testing and the joint inspection were analyzed.Results HC2-HPV-DNA positive rate increased with the increase of cervical lesion severity ,there had statistical difference(P <0.05),sensitivity,specificity,and positive predictive value of HC2-HPV-DNA with LCT joint detection were 78.1%,40% and 95.3%,respectively,the sensitivity significantly increased than single detection (P <0.05).Conclusion HC2-HPV-DNA is a good reference indicator in cervical lesion screening ,the accuracy increases with the increase of the degree of pathological changes,HC2-HPV-DNA and LCT joint detection can improve the accuracy of the diagnosis of cervical cancer and precancerous lesions.%目的:观察分析采用2代杂交捕获法检测高危人乳头状瘤病毒 DNA(HC2-HPV-DNA)对宫颈癌病变筛查中的应用及准确性。方法选取110例疑似宫颈癌患者为研究对象,分别进行宫颈液基薄层细胞学(LCT)检查、HC2-HPV-DNA 检测及宫颈组织病理活检,观察分析 LCT 检查、HC2-HPV-DNA 检测和二者联合检查阳性率、敏感性、特异性及预测值情况。结果随着宫颈病变严重程度的增加,HC2-HPV-DNA 的阳性率升高,差异有统计学义意(P <0.05), HC2-HPV-DNA 与 LCT 联合检测敏感性、特异性、阳性预测值分别为78.1%、40.0%、95.3%,敏感性较单独检测显著提高(P <0.05)。结论HC2-HPV-DNA 是宫颈癌病变筛选的良好参照指标,随病变程度的增加,

  6. The application of HPV-DNA detection combined with VIA test in screening of precancerous lesion of uterine cervix%高危型HPV检测联合肉眼醋酸试验在宫颈癌前病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    赵金娟

    2012-01-01

    目的:研究在宫颈癌前病变筛查中采用高危型HPV检测联合肉眼醋酸试验的应用效果.方法:选取2007年8月~2010年2月于我院治疗的可疑宫颈癌前病变患者532例,应用5%醋酸和Lugol's 液涂抹于宫颈表面,筛选出有异常着色的患者320例,再采用实时荧光定量PCR技术检测高危型HPV病毒感染情况.结果:320例肉眼醋酸试验异常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染190例,阳性率为59.38%;212例肉眼醋酸试验正常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染18例,阳性率为8.49%.结论:肉眼醋酸试验联合高危型HPV检测可及时、有效地发现宫颈癌前病变,可作为宫颈癌前病变筛查方案应用于临床.%Objective: To study the efficiency of HPV-DNA detection combined with visual inspection with acetic acid (VIA) test in screening of precancerous lesions of uterine cervix. Methods: 532 suspected precancerous lesion of uterine cervix patients in our hospital from August 2007 to February 2010 were selected. 5% acetic acid and Lugol's liquid were painted on the surface of cervix of all patients, and 320 cases of patients were screened by the abnormal color. Then the re -al-time fluorescence quantitative polymerase chain reaction technology was used to test high risk HPV infection in all pa -tients. Results: High risk HPV infection test of 320 patients who were showed abnormal color in VIA test was showed that 190 cases got HPV positive infection, the positive rate was 59.38%. High risk HPV infection test of 212 patients who were showed normal color in VIA test was showed that 18 cases got HPV positive infection, the positive rate was 8.49%. Conclusion: VIA test combined with HPV-DNA checking can timely and effectively discover precancerous lesion, which can be used in clinic as precancerous lesion screening plan.

  7. Incidence of cervical lesions in Danish women before and after implementation of a national HPV vaccination program

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Dehlendorff, Christian; Junge, Jette;

    2014-01-01

    PURPOSE: Approximately 7% of cervical cancers and about 50% of high-grade cervical precursor lesions are caused by human papillomavirus (HPV) types 16 and 18. Denmark introduced the quadrivalent HPV vaccine into the vaccination program for 12-year-old girls in 2009 supplemented by a first catch......+) and cervical intraepithelial neoplasia grade 2 or worse (CIN2+) increased in all age groups in 2000-2010. After introduction of the quadrivalent HPV vaccine into the vaccination program, the incidence of atypia+ decreased significantly in women younger than 18 years (EAPC -33.4%; 95% CI -49.6; -12.0) and in 18...... in age groups with high HPV vaccine coverage, indicating an early effect of HPV vaccination....

  8. Differential methylation of E2 binding sites in episomal and integrated HPV 16 genomes in preinvasive and invasive cervical lesions.

    Science.gov (United States)

    Chaiwongkot, Arkom; Vinokurova, Svetlana; Pientong, Chamsai; Ekalaksananan, Tipaya; Kongyingyoes, Bunkerd; Kleebkaow, Pilaiwan; Chumworathayi, Bandit; Patarapadungkit, Natcha; Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-05-01

    Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation of the squamous epithelial cells at the uterine cervix. The HPV E2 protein is a key transcriptional regulator of the E6-E7 genes. It binds to four E2 binding sites (E2BSs 1-4) in the viral upstream regulatory region (URR). Modification of E2 functions, for example, by methylation of E2BSs is hypothesized to trigger enhanced expression of the viral E6-E7 oncogenes. In the majority of HPV-transformed premalignant lesions and about half of cervical carcinomas HPV genomes persist in an extra-chromosomal, episomal state, whereas they are integrated into host cells chromosomes in the remaining lesions. Here we compared the methylation profile of E2BSs 1-4 of the HPV 16 URR in a series of 18 HPV16-positive premalignant lesions and 33 invasive cervical cancers. CpGs within the E2BSs 1, 3, and 4 were higher methylated in all lesions with only episomal HPV16 genomes compared with lesions displaying single integrated copies. Samples with multiple HPV16 integrated copies displayed high methylation levels for all CpGs suggesting that the majority of multiple copies were silenced by extensive methylation. These data support the hypothesis that differential methylation of the E2BSs 1, 3 and 4 is related to the activation of viral oncogene expression in cervical lesions as long as the viral genome remains in the episomal state. Once the virus becomes integrated into host cell chromosomes these methylation patterns may be substantially altered due to complex epigenetic changes of integrated HPV genomes.

  9. Diagnostic value of cervical cytological examination, HPV-DNA test combined with colposcopy examination in cervical lesions diagnosis%宫颈细胞学检查、HPV-DNA检测配合阴道镜检查对子宫颈病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李玉欢

    2013-01-01

    目的 分析与探讨宫颈细胞学检查、HPV-DNA(人乳头瘤病毒基因检测)检测配合阴道镜检查对于子宫颈病变的诊断价值.方法 选取本院2010年8月至2012年8月期间收治的接受子宫颈病变筛查的妇女共1020例,对其实施宫颈细胞学检查和HPV-DNA配合阴道镜检查,检查结果与组织学金标准进行比对,以对比两种方法的准确性与诊断价值.结果 采取HPV-DNA配合阴道镜检查为异常结果的检出率较宫颈细胞学检查更高,差异具有统计学意义(P<0.05),前者检出异常共64例,后者检出异常共34例.结论 宫颈细胞学检查、HPV-DNA检测以及阴道镜检查对于子宫颈病变均有一定的诊断价值,而采用HPV-DNA检测配合阴道镜检查能够帮助医生较早地发现患者的宫颈癌前病变,为患者的治疗方案的选择提供科学依据.%Objective To analyze and discuss the diagnostic value of cervical cytological examination,HPV-DNA test combined with colposcopy examination in diagnosis of cervical lesions.Methods From August 2010 to August 2012 in our hospital a total of 1020 cases of women who underwent cervical diseases screening,were implemented with cervical cytological examination and HPV-DNA combined with colposcopy examination,the correlated pathological results were compared with the gold standard in accuracy and diagnostic value of two methods.Results The HPV-DNA abnormal results detection rate was higher than that of cervical cytological examination combined with colposcopy examination,with statistically significant difference,former found 64 abnormal cases,the latter examination found 34 cases of abnormal.Conclusion Cervical cytological examination,HPV-DNA testing combined with colposcopy examination for cervical lesions has certain diagnostic value,and the combined use of HPV-DNA testing and colposcopy examination could help doctors find early precancerous lesion of cervical cancer,and provide scientific basis for the

  10. Restoration of non-carious cervical lesions with ceramic inlays: A possible model for clinical testing of adhesive cements

    OpenAIRE

    Michal Staninec; Tsuji, Grant H.

    2012-01-01

    Introduction: There are many luting cements coming to market which claim to be adhesive, but there is no clinical protocol currently for testing these claims. There is a standardized protocol for testing direct restorations bonded to dentin and it is used extensively. Case Report: We describe a clinical procedure for restoring a non-carious cervical lesion (NCCL) with a ceramic inlay using Computer-Aided Design and Computer-Aided Manufacturing (CAD-CAM) technology and an adhesive resin cement...

  11. Cervical pre-malignant lesions in HIV infected women attending Care and Treatment Centre in a tertiary hospital, Dar es Salaam, Tanzania.

    Science.gov (United States)

    Balandya, Belinda S; Pembe, Andrea B; Mwakyoma, Henry A

    2011-09-01

    The aims of this study was to determine proportion of HIV infected women with cervical pre-malignant lesions; and compare the use of Visual Inspection of the cervix after application of Acetic acid (VIA) and Papanicolau (Pap) smear in screening for cervical premalignant lesions in HIV positive women attending Care and Treatment Centre (CTC) at Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. A total of 316 women aged 18-70 years had a Pap smear taken for cytology, followed by spraying onto the cervix with 4% acetic acid and then inspecting it. Cytology was considered negative when there was no Cervical Intraepithelial Neoplasia (CIN) lesion reported from the Pap smear taken, and positive if CIN lesion 1, 2 or 3 was reported. Detection of a well-defined, opaque acetowhite lesion close to the squamocolumnar junction or close to the external cervical os constituted a positive VIA. Out of 316 women, 132 women had acetowhite lesions on VIA, making the proportion of abnormal cervical lesions to be 42.4%. One hundred and one out of 312 women (32.4%) had CIN lesions detected on Pap smear. The proportion of agreement between these two tests was 0.3. The proportion of agreement was moderate in women with advanced WHO HIV clinical stage of the disease and in women not on ART (Anti Retroviral Therapy). Women with CD-4 count less than 200 cells/mm3 had more abnormal cervical lesions. There is considerable proportion of HIV positive women with premalignant lesions of the cervix. Considering the proportion of HIV women with abnormal lesions and the difficulty in logistics of doing Pap smear in low resource settings, these results supports the recommendation to introduce screening of premalignant lesions of the cervix using VIA to all HIV infected women.

  12. Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions

    Science.gov (United States)

    Belfort-Mattos, Patrícia Napoli; Focchi, Gustavo Rubino de Azevedo; Ribalta, Julisa Chamorro Lascasas; Megale De Lima, Tatiana; Nogueira Carvalho, Carmen Regina; Kesselring Tso, Fernanda; De Góis Speck, Neila Maria

    2016-01-01

    VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P < 0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P = 0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P = 0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression. PMID:27313335

  13. CCR2 and CCR5 genes polymorphisms in women with cervical lesions from Pernambuco, Northeast Region of Brazil: a case-control study.

    Science.gov (United States)

    Santos, Erinaldo Ubirajara Damasceno dos; Lima, Géssica Dayane Cordeiro de; Oliveira, Micheline de Lucena; Heráclio, Sandra de Andrade; Silva, Hildson Dornelas Angelo da; Crovella, Sergio; Maia, Maria de Mascena Diniz; Souza, Paulo Roberto Eleutério de

    2016-03-01

    Polymorphisms in chemokine receptors play an important role in the progression of cervical intraepithelial neoplasia (CIN) to cervical cancer (CC). Our study examined the association of CCR2-64I (rs1799864) andCCR5-Δ32 (rs333) polymorphisms with susceptibility to develop cervical lesion (CIN and CC) in a Brazilian population. The genotyping of 139 women with cervical lesions and 151 women without cervical lesions for the CCR2-64I and CCR5-Δ32 polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism. The individuals carrying heterozygous or homozygous genotypes (GA+AA) for CCR2-64I polymorphisms seem to be at lower risk for cervical lesion [odds ratio (OR) = 0.37, p = 0.0008)]. The same was observed for the A allele (OR = 0.39, p = 0.0002), while no association was detected (p > 0.05) with CCR5-Δ32 polymorphism. Regarding the human papillomavirus (HPV) type, patients carrying the CCR2-64Ipolymorphism were protected against infection by HPV type 16 (OR = 0.35, p = 0.0184). In summary, our study showed a protective effect ofCCR2-64I rs1799864 polymorphism against the development of cervical lesions (CIN and CC) and in the susceptibility of HPV 16 infection.

  14. Cervical Lesions in Women Younger than 25 Years Lesiones de cuello uterino en mujeres menores de 25 años

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available

    Background: Cervical cancer is essentially a sexually transmitted disease related to human papillomavirus infection. This disease’s incidence has been globally enhanced, particularly in increasingly younger women. Objective: To determine cervical lesions in women younger than 25 years. Methods: An observational, descriptive and correlational study including 248 patients registered at the Pathological Anatomy Service of the General University Hospital Dr. Gustavo Aldereguía Lima of Cienfuegos was conducted from January to December 2007. It also included the first quarter of 2008. The following variables were analyzed: patient’s age, age at first intercourse, number of sexual partners, type and degree of injury, and reason for attending consultation. Results: Women over 21 years old and those who had had 2 and 3 sexual partners predominated. The main reason for attending consultation was cervicitis (84, 7%. In 22.6% of women grade I intraepithelial neoplasia was detected, while grade II and III were detected in 41, 9% and 33, 1%, respectively. In 90% of younger patients the lesion was malignant. Conclusions: The presence of cervical lesions in women under 25 years is not low and neither is the frequency of malignant lesions.

    Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a

  15. 围绝经期宫颈病变所致阴道流血%Perimenopausal abnormal vaginal bleeding caused by cervical lesions

    Institute of Scientific and Technical Information of China (English)

    王登凤; 张国楠

    2012-01-01

    围绝经期妇女中,不规则阴道流血是一个最常见、也是最易被忽视的症状,宫颈病变是其主要原因之一,主要包括了宫颈炎、宫颈炎症相关疾病、宫颈上皮内瘤变、宫颈癌、宫颈肌瘤、宫颈结核、宫颈妊娠等,可以通过妇科查体、宫颈脱落细胞学检查、宫颈活检等简单手段在短时间内得到确诊.%For perimenopausal women, abnormal vaginal bleeding is the most common symptom, which is also the most neglected one. In various causes, cervical lesion is the one which can be diagnosed by some simple methods, e. g. gynecological examination , cervical cytology, and cervical biopsy. Cervical lesions in this review include: cervicitis, cervicitis related disease, cervical intraepithelial neoplasia, cervical cancer, cervical myoma, tuberculosis of cervix, cervical pregnancy, etc.

  16. Cervical myelopathy due to single level disc herniation presenting as intramedullary mass lesion: What to do first?

    Science.gov (United States)

    Ekşi, Murat Şakir; Özcan Ekşi, Emel Ece; Yılmaz, Baran; Toktaş, Zafer Orkun; Konya, Deniz

    2015-01-01

    Cervical myelopathy (CM) is mostly a degenerative process ending in myelopathic and/or radiculopathic syndromes. On T2-weighted magnetic resonance imaging (MRI), CM appears as a hyperintense area near the spondylotic spine. This high intensity signal depends on the impact of outer forces and their duration. It also determines the prognosis of the surgical candidate. A 40-year-old male patient admitted to our clinic with right upper extremity weakness and hypoesthesia that had started 2 months earlier. On neurological examination there was 2/5 motor weakness of right biceps brachii, and hypoesthesia over right C6 dermatome. Right upper extremity deep tendon reflexes were hypoactive, but lower ones were hyperactive. After clinical and radiological work-up, preliminary diagnosis was directed to a spinal intramedullary tumor. Total resection of the herniated cervical disc fragment and the mass lesion was managed. Pathology of the mass lesion was compatible with subacute infarct tissue and inflammatory response. Final diagnosis was CM under effect of cervical disc herniation. Contrast-enhanced spinal cord myelopathic lesions are very rare and resemble much more tumors and inflammatory processes. However, the principal treatment approach totally differs depending on pathology. When there are both a disc herniation and a high clinical suspicion; biopsy should be delayed. The most probable solution will be surgery for the disc disease with thorough preoperative scanning of vascular malformations; clinical and radiological close follow-up after surgery. Biopsy or surgical resection can be performed if patient deteriorates despite the primary surgery. PMID:25972718

  17. HPV genotypes in high grade cervical lesions and invasive cervical carcinoma as detected by two commercial DNA assays, North Carolina, 2001-2006.

    Directory of Open Access Journals (Sweden)

    Susan Hariri

    Full Text Available BACKGROUND: HPV typing using formalin fixed paraffin embedded (FFPE cervical tissue is used to evaluate HPV vaccine impact, but DNA yield and quality in FFPE specimens can negatively affect test results. This study aimed to evaluate 2 commercial assays for HPV detection and typing using FFPE cervical specimens. METHODS: Four large North Carolina pathology laboratories provided FFPE specimens from 299 women ages18 and older diagnosed with cervical disease from 2001 to 2006. For each woman, one diagnostic block was selected and unstained serial sections were prepared for DNA typing. Extracts from samples with residual lesion were used to detect and type HPV using parallel and serial testing algorithms with the Linear Array and LiPA HPV genotyping assays. FINDINGS: LA and LiPA concordance was 0.61 for detecting any high-risk (HR and 0.20 for detecting any low-risk (LR types, with significant differences in marginal proportions for HPV16, 51, 52, and any HR types. Discordant results were most often LiPA-positive, LA-negative. The parallel algorithm yielded the highest prevalence of any HPV type (95.7%. HR type prevalence was similar using parallel (93.1% and serial (92.1% approaches. HPV16, 33, and 52 prevalence was slightly lower using the serial algorithm, but the median number of HR types per woman (1 did not differ by algorithm. Using the serial algorithm, HPV DNA was detected in >85% of invasive and >95% of pre-invasive lesions. The most common type was HPV16, followed by 52, 18, 31, 33, and 35; HPV16/18 was detected in 56.5% of specimens. Multiple HPV types were more common in lower grade lesions. CONCLUSIONS: We developed an efficient algorithm for testing and reporting results of two commercial assays for HPV detection and typing in FFPE specimens, and describe HPV type distribution in pre-invasive and invasive cervical lesions in a state-based sample prior to HPV vaccine introduction.

  18. Diagnosis of cervical cancer with transvaginal color Doppler sonography

    Directory of Open Access Journals (Sweden)

    Li-bo DENG

    2011-09-01

    Full Text Available Objective To investigate the imaging features of cervical cancer by transvaginal color Doppler sonography(TVCS,and evaluate the diagnostic value of TVCS.Methods A hundred and thirty cases of cervical intraepithelial neoplasia(CIN grade Ⅰ-Ⅱ and cervical cancer,diagnosed by Thinprep cytologic test(TCT,cervical biopsy and pathological examination,received TVCS examination.The image characters and color Doppler flow imaging(CDFI were collected and analyzed.Another 41 cases with normal cervices as determined by inspection and cytological examination were involved as control.Results In order of normal cervix,CIN,cancer in situ and cervical cancer,the cervical diameter showed a tendency of increase,also with an increase incidence of low-level echo focus in cervix.As a specific image of cervical cancer,the low level echo focus occurred only in cervical cancer with a specificity of 100%.The absence of mucosal line in cervical canal was a specific character of stage Ⅱ cervical cancer with a specificity of 100%.CDFI and resistance index(RI revealed that the local blood flow was more abundant in invasive cancer than in CIN and cancer in situ,and significant difference was found between stage Ⅰ and stage Ⅱ cervical cancer(P < 0.05.The sensitivity and specificity of enlarged cervical diameters in diagnosis of cervical cancer were 89.1% and 82.8%.The specificity of cervical low level echo focus in diagnosis of cervical cancer and invasive cervical cancer were 100% and 94.8%,respectively.The specificity of abundant blood flow in dendritic form in diagnosis of invasive cervical cancer was 100%.Conclusions Invasive cervical cancer may present several specific features in TVCS images.TVCS examination is of high reliability in diagnosis of invasive cervical cancer,but is not so reliable in diagnosing precancerous lesion and preinvasive cancer.Combined with other auxiliary examinations,TVCS could be considered as one of the methods to diagnose cervical

  19. A STUDY ON CERVICAL PAP SMEAR EXAMINATION IN PATIENT LIVING WITH HIV

    Directory of Open Access Journals (Sweden)

    B M Jha

    2012-02-01

    Full Text Available Background: Decline in morbidity & mortality due to cervical cancer in developed countries can be mainly attributed to early detection of precancerous & cancerous lesions due to extensive screening programmeof cervical Pap smear examination. HPV infection is a known etiological agent for cervical cancer. HIV infected women are at higher risk of contracting HPV infection due to immune compromised status. Objective: Present study has been undertaken mainly to detect precancerous & cancerous lesions as well as inflammatory lesions in female patients living with HIV & to emphasize the fact that Pap smear examination should be established as a part of routine protocol for examination in these women. Methods: The study was carried out on 407 HIV infected females attending Integrated Counseling &Testing Centre of government institute. As controls, 200 females (not falling under high risk category, attending the Obstetrics& Gynecology OPD with various gynecological complaints were taken & results were compared. Results: Squamous cell abnormalities were found about four times high as compared to control group(Pvalue <0.05. High incidences of squamous cell abnormalities were noted in patients with high parity (parity three or more. Conlcusion: Regular gynecological examination including Pap smear examinations is highly recommended for HIV infected females.Pap smear examination is a simple, cheap, safe & practical diagnostic tool for early detection of cervical cancer in high risk population. [National J of Med Res 2012; 2(1.000: 81-84

  20. DAPK1, MGMT and RARB promoter methylation as biomarkers for high-grade cervical lesions

    OpenAIRE

    Sun, Yin; Li, Shu; Shen, Keng; Ye, Shuang; Cao, Dongyan; Yang, Jiaxin

    2015-01-01

    Gene promoter methylation may be used a potential biomarker for detecting solid tumor including cervical cancer. Here, we used methylation sensitive-high resolution melting (MS-HRM) analysis to detecting promoter methylation ratios of DAPK1, MGMT and RARB gene in patients with different cervical disease grade. The detection of gene promoter methylation was conducted in two hundred fifty patients’ samples including normal cytology (n=48), cervical intraepithelial neoplasia grade 1 (CIN1, n=54)...

  1. Association between bacterial vaginosis and cervical intraepithelial neoplasia: systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Evy Gillet

    Full Text Available OBJECTIVE: Bacterial vaginosis (BV, the most common vaginal disorder among women of reproductive age, has been suggested as co-factor in the development of cervical cancer. Previous studies examining the relationship between BV and cervical intra-epithelial neoplasia (CIN provided inconsistent and conflicting results. The aim of this study is to clarify the association between these two conditions. METHODS: A systematic review and meta-analysis were conducted to summarize published literature on the association between BV and cervical pre-cancerous lesions. An extensive search of electronic databases Medline (Pubmed and Web of Science was performed. The key words 'bacterial vaginosis' and 'bacterial infections and vaginitis' were used in combination with 'cervical intraepithelial neoplasia', 'squamous intraepithelial lesions', 'cervical lesions', 'cervical dysplasia', and 'cervical screening'. Eligible studies required a clear description of diagnostic methods used for detecting both BV and cervical pre-cancerous lesions. Publications were included if they either reported odds ratios (OR and corresponding 95% confidence intervals (CI representing the magnitude of association between these two conditions, or presented data that allowed calculation of the OR. RESULTS: Out of 329 articles, 17 cross-sectional and 2 incidence studies were selected. In addition, two studies conducted in The Netherlands, using the national KOPAC system, were retained. After testing for heterogeneity and publication bias, meta-analysis and meta-regression were performed, using a random effects model. Although heterogeneity among studies was high (χ(2 = 164.7, p<0.01, I(2 = 88.5, a positive association between BV and cervical pre-cancerous lesions was found, with an overall estimated odds ratio of 1.51 (95% CI, 1.24-1.83. Meta-regression analysis could not detect a significant difference between studies based on BV diagnosis, CIN diagnosis or study population

  2. Preprocessing: A Step in Automating Early Detection of Cervical Cancer

    CERN Document Server

    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Uterine Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, colposcopic images or cervigrams are acquired in raw form. They contain specular reflections which appear as bright spots heavily saturated with white light and occur due to the presence of moisture on the uneven cervix surface and. The cervix region occupies about half of the raw cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. Therefore we focus on the cervical borders, so that we have a geometric boundary on the relevant image area. Our novel technique eliminates the SR, identifies the region of interest and makes the cervigram ready for segmentation algorithms.

  3. Preprocessing for Automating Early Detection of Cervical Cancer

    CERN Document Server

    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Uterine Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, colposcopic images or cervigrams are acquired in raw form. They contain specular reflections which appear as bright spots heavily saturated with white light and occur due to the presence of moisture on the uneven cervix surface and. The cervix region occupies about half of the raw cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. Therefore we focus on the cervical borders, so that we have a geometric boundary on the relevant image area. Our novel technique eliminates the SR, identifies the region of interest and makes the cervigram ready for segmentation algorithms.

  4. DAPK1, MGMT and RARB promoter methylation as biomarkers for high-grade cervical lesions

    Science.gov (United States)

    Sun, Yin; Li, Shu; Shen, Keng; Ye, Shuang; Cao, Dongyan; Yang, Jiaxin

    2015-01-01

    Gene promoter methylation may be used a potential biomarker for detecting solid tumor including cervical cancer. Here, we used methylation sensitive-high resolution melting (MS-HRM) analysis to detecting promoter methylation ratios of DAPK1, MGMT and RARB gene in patients with different cervical disease grade. The detection of gene promoter methylation was conducted in two hundred fifty patients’ samples including normal cytology (n=48), cervical intraepithelial neoplasia grade 1 (CIN1, n=54), cervical intraepithelial neoplasia grade 2 (CIN2, n=47), cervical intraepithelial neoplasia grade 3 (CIN3, n=56) and cervical squamous cell carcinomas (SCS, n=45). We found there were a significant positive correlation between the promoter methylation status of DAPK1 and cervical disease grade (P=0.022). In addition, the methylated promoters of DAPK1 combined with MGMT, MGMT combined with RARB, DAPK1 combined with RARB were positive correlated with cervical disease grade (P < 0.05). All three genes promoters methylated were positive correlated with cervical disease grade (P < 0.001). Receiver operating characteristic (ROC) curves was conducted to evaluate whether the three genes methylation could be used to be a potential marker for diagnosing high grade cervical disease (HSIL and SCC). The cutoff values for the methylation rates of all these genes were 0-5%. Regrettably, only the methylation of MGMT combined with DAPK1 gave 43.4% sensitivity and 68.6% specificity. The current results indicated that MS-HRM-based testing for DNA methylations of MGMT plus DAPK1 genes holds some promise for high grade cervical disease screening. PMID:26823825

  5. Randomized clinical trial of four adhesion strategies in cervical lesions: 12-month results.

    Science.gov (United States)

    de Paula, Eloisa Andrade; Tay, Lidia Yileng; Kose, Carlos; Mena-Serrano, Alexandra; Reis, Alessandra; Perdigão, Jorge; Loguercio, Alessandro D

    2015-01-01

    The aim of this study was to evaluate the 6- and 12-month clinical performance of four adhesion strategies from the same manufacturer (Kerr) in non-carious cervical lesions (NCCLs) using two evaluation criteria. Thirty-five patients, with at least four NCCLs each, participated in this study. After samplesize calculation, 180 restorations were assigned to one of the following groups: OFL (Optibond FL), OSP (Optibond Solo Plus), XTR (Optibond XTR), and AIO (Optibond All-In-One). The composite resin Filtek Supreme Ultra (3M ESPE) was placed incrementally. The restorations were evaluated at baseline, after 6 months, and after 12 months, using both the FDI and the USPHS-modified criteria. Statistical analyses were performed with Friedman repeated measures, ANOVA by rank, and the McNemar test for significance in each pair (α=0.05). Six restorations (2 for OFL, 1 for OSP, 2 for XTR, and 1 for AIO) were lost at 12 months (P>0.05 for both evaluation criteria). Marginal staining was observed in seven restorations using the FDI criteria (P>0.05) and three restorations using the USPHSmodified criteria (P>0.05). Eight restorations (2 for OSP, 3 for XTR, and 3 for AIO) were classified as Bravo for marginal adaptation using the USPHSmodified criteria (P>0.05). However, 62 restorations (14 for OFL, 12 for OSP, 15 for XTR, and 21 for AIO) were classified as Bravo using the FDI criteria (P>0.05). The four adhesion strategies showed similar clinical retention at 6 and 12 months. The FDI evaluation criteria tend to be more sensitive than the USPHS-modified criteria. PMID:25625130

  6. Clinical and epidemiological correlations between the infection with HPV 16 and HPV 18 and female cervical lesions.

    Science.gov (United States)

    Stoian, M; Repanovici, R; Corniţescu, F

    1995-01-01

    A number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (HPV) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti E4 and E7 HPV protein antibodies. The findings were compared with the histologic diagnosis. Fifty-six per cent of specimens contained HPV DNA sequences. In 47% of specimens from cervical carcinoma, HPV 11 was detected in 4 cases, HPV 16 in 21 cases, and HPV 18 in 7 cases. Serum antibodies against HPV 16 E4 and HPV 16 E7 occurred in all the cases of uterine carcinoma, in 4 of 10 cases of CIN I-II, and in 3 of 5 sera obtained from apparently healthy women. The analysis of risk factors disclosed the early onset of sexual activity, a relatively high number of births and abortions before the age of 22 years, the use of oral oestroprogestative contraceptive agents, the presence in anamnesis of genital infections with bacterial flora--Candida albicans, Trichomonas vaginalis, Chlamydia trachomatis, Mycoplasma, etc. Our results showed that HPV typing by nucleic acid hybridization was useful for differentiating low- from high-risk cervical lesions and also tried to elucidate the risk factors associated with HPV infections and progression to malignancy. PMID:9179967

  7. HPV prevalence and type distribution in women with or without cervical lesions in the Northeast region of Romania

    Directory of Open Access Journals (Sweden)

    Ursu Ramona

    2011-12-01

    Full Text Available Abstract Background Cervical cancer is a major public health problem worldwide. While Romania has the highest incidence of cervical cancer in Europe, the prevalence of HPV has not been evaluated. We report the first data on HPV prevalence and type distribution in Northeast Romania. Methods HPV prevalence and genotype distribution was investigated in 514 consecutively women with or without cervical lesions in Northeast Romania. Genotyping was performed with Linear Array Genotyping/Roche kit. Results In our study group, 192/514 (37.4% patients were positive for HPV (infected with single and with multiple HPV types. Most frequent types were: 16 (10.5%, 53 (5.44%, 51 (5.05%, 52 (4.08% 18 (2.91% and 31 (2.73%. Conclusions Infection with high risk types of HPV is common in Northeast Romania. Enhanced and systematic screening for cervical cancer is needed. Our results call for the implementation of a National HPV vaccine program in Romania.

  8. Effect of three types of TCM treatments on precancerous lesions of rats with cell proliferation%3种中医治法对胃癌前病变大鼠胃黏膜细胞增殖的影响

    Institute of Scientific and Technical Information of China (English)

    蒋时红; 刘旺根; 张文娴; 刘燕

    2011-01-01

    Objective: To compare the three types of TCM treatments on precancerous lesions of rats with cell proliferation and discuss the mechanism. Methods: Adopt the chemical damage and other methods to make PLGC model, and divided rats into control group and model group which was divided into strengthening the spleen and replenishing qi group, promoting blood circulation and removing blood stasis group and nourishing Yin and relax channels and collaterals group. The expression of PCNA and Cyclin D1 protein was detected by immunohistochemistry after treated for 6 weeks respectively. Results: ' Compared with normal group, PI and the expression of PCNA and Cyclin Dl were significantly raise in those be made model rats (P<0.05). Compared with model group, three groups of TCM can significantly reduced PI and the expression of PCNA and Cyclin Dl at rat gastric mucosal cell(P<0.05). The best of three is nourishing Yin and relax channels and collaterals therapy. Conclusion: Three of TCM can reduced the expression of PCNA and Cyclin Dl at gastric mucosal cell, and inhibited the DNA synthesis process, thereby inhibiting cell proliferation.%目的:比较3种中医治法对胃癌前病变大鼠胃黏膜细胞增殖的影响,并探讨其机制.方法:采用以化学损伤为主的综合法复制大鼠胃癌前病变模型,将大鼠随机分为正常对照组和造模组,造模成功后再将造模组分为模型对照组、健脾益气组、活血化瘀组、养阴荣络组,中药治疗6周,采用免疫组织化学的方法检测大鼠胃黏膜组织内细胞增殖核抗原( PCNA)及细胞周期素D1 (Cyclin D1)的表达情况.结果:与正常对照组相比,各参加造模大鼠胃黏膜细胞的增殖指数PI(%)、PCNA和Cyclin D1的表达量显著升高(P<0.05).与模型对照组相比,3个用药组增殖指数PI、PCNA和Cyclin D1表达量均明显减低(P<0.05).3个用药组之间进行两两比较,也具有显著性差异(P<0.05),且以养阴荣络组最低.结论:3种治

  9. Correlation of Trefoil factor 3 expression in gastric carcinoma and precancerous lesions with angiogenesis%TFF3在胃癌、癌前病变中的表达及与血管生成的关系

    Institute of Scientific and Technical Information of China (English)

    王臣臣; 顾晓萌; 王妍; 黄山英

    2013-01-01

    目的 研究三叶因子3(TFF3)在胃癌及癌前病变中的表达情况,探讨其与胃癌的发生和血管生成的关系.方法 采用免疫组织化学方法检测26例浅表性胃炎、25例肠上皮化生、20例不典型增生、66例胃癌组织中TFF3的表达,用CD34标记血管内皮,计数微血管密度(MVD).结果 胃癌组TFF3蛋白表达低于肠上皮化生组、不典型增生组,3组之间差异无统计学意义(P>0.05),但均明显高于浅表性胃炎组(P<0.01);胃癌组MVD值明显高于其他3组(P<0.01),肠上皮化生组、不典型增生组间差异无统计学意义(P>0.05),但明显高于浅表性胃炎组(P<0.01),TFF3表达与胃癌淋巴结转移和TNM分期有关(P<0.05),TFF3表达与MVD呈显著正相关(r=0.672),阳性表达者的MVD值明显高于阴性表达者(P<0.01).结论 TFF3可能参与了胃癌发生发展、浸润转移和血管形成,其表达水平可作为早期预测胃癌发生发展和预后的有用指标.%Objective To investigate the expression of TFF3 in gastric carcinoma and precancerous lesions, and explore the correlation in gastric carcinogenesis and angiogenesis. Methods The expression of TFF3 was detected by immuno-histochemistry in 26 cases of superficial gastritis, 25 cases of intestinal metaplasia, 20 cases of atypical hyperplasia and 66 cases of gastric carcinoma. MVD was detected by immunohistochemistry using anti-CD34 antibody. Results The expression of TFF3 was lower in gastric carcinoma than in intestinal metaplasia and atypical hyperplasia, with higher expressions than that in superficial gastritis (P 0.05) , with higher expressions than that in superficial gastritis(P < 0.01). The expression of TFF3 was correlated with lymph node metastasis and TNM staging(P < 0.05). The expressions of TFF3 and MVD were positively correlated (r = 0. 672). MVD with positive expression of TFF3 was higher than that with negative expression (P< 0. 01). Conclusion TFF3 may play important roles in

  10. Quadrivalent HPV vaccine effectiveness against high-grade cervical lesions by age at vaccination: A population-based study.

    Science.gov (United States)

    Herweijer, Eva; Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim-Dahlström, Lisen

    2016-06-15

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006-2013 and aged 13-29 (n = 1,333,691) was followed for vaccination and histologically confirmed high-grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1-IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18-0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46-0.64) and 22% (IRR = 0.78, 95%CI = 0.65-0.93) for those initiating vaccination at ages 17-19, and at ages 20-29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long-term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer.

  11. Cervical myelopathy due to single level disc herniation presenting as intramedullary mass lesion: What to do first?

    Directory of Open Access Journals (Sweden)

    Murat Sakir Eksi

    2015-01-01

    Full Text Available Cervical myelopathy (CM is mostly a degenerative process ending in myelopathic and/or radiculopathic syndromes. On T2-weighted magnetic resonance imaging (MRI, CM appears as a hyperintense area near the spondylotic spine. This high intensity signal depends on the impact of outer forces and their duration. It also determines the prognosis of the surgical candidate. A 40-year-old male patient admitted to our clinic with right upper extremity weakness and hypoesthesia that had started 2 months earlier. On neurological examination there was 2/5 motor weakness of right biceps brachii, and hypoesthesia over right C6 dermatome. Right upper extremity deep tendon reflexes were hypoactive, but lower ones were hyperactive. After clinical and radiological work-up, preliminary diagnosis was directed to a spinal intramedullary tumor. Total resection of the herniated cervical disc fragment and the mass lesion was managed. Pathology of the mass lesion was compatible with subacute infarct tissue and inflammatory response. Final diagnosis was CM under effect of cervical disc herniation. Contrast-enhanced spinal cord myelopathic lesions are very rare and resemble much more tumors and inflammatory processes. However, the principal treatment approach totally differs depending on pathology. When there are both a disc herniation and a high clinical suspicion; biopsy should be delayed. The most probable solution will be surgery for the disc disease with thorough preoperative scanning of vascular malformations; clinical and radiological close follow-up after surgery. Biopsy or surgical resection can be performed if patient deteriorates despite the primary surgery.

  12. TCT联合DNA定量细胞学检查对宫颈病变临床诊断价值研究%The study of TCT and DNA quantitive cytology in the diagnosis of cervical lesions.

    Institute of Scientific and Technical Information of China (English)

    刘桂依; 黄艳美

    2011-01-01

    cervical cytology, DNA quantitative cytology and pathology can provide early detection of cervical cancer and precancerous lesions.

  13. Prevalence and risk factors for non-carious cervical lesions in children attending special needs schools in India.

    Science.gov (United States)

    Kumar, Sandeep; Kumar, Arunoday; Debnath, Nitai; Kumar, Amit; K Badiyani, Bhumika; Basak, Debashish; S A Ali, Mohamed; B Ismail, Mohammed

    2015-03-01

    We assessed the prevalence and risk factors for development of non-carious cervical lesions (NCCLs) in children attending special needs schools in India. The participants were 395 children aged 12-15 years with disabilities in learning, communication, physical function, and/or development. A questionnaire was designed in order to collect information on sociodemographic characteristics, oral hygiene practices, dietary habits, and risk factors for NCCLs. The chi-square test, bivariate analysis, and logistic regression analysis were used to analyze the data. Presence of NCCLs was associated with use of toothpowder or other materials for teeth cleaning, use of harder toothbrushes, use of a horizontal scrub technique for toothbrushing, consumption of a vegetarian diet, and greater consumption of lemon. The overall prevalence of NCCLs was 22.7%. Most lesions involved minimal loss of contour or defects health care among children attending special needs schools. PMID:25807907

  14. Quality of life in women with cervical precursor lesions and cancer:a prospective, 6-month, hospital-based study in China

    Institute of Scientific and Technical Information of China (English)

    Zhi-Mei Zhao; Xiong-Fei Pan; Si-Han Lv; Yao Xie; Shao-Kai Zhang; You-Lin Qiao; Xiao-Rong Qi; Chun-Xia Yang; Fang-Hui Zhao

    2014-01-01

    The overal survival of patients with cervical cancer has improved due to detection at an early stage and availability of comprehensive treatments in China. As patients’ lives prolonged, it is important to understand their health-related quality of life (QoL) during and after treatment. We used the EQ-5D questionnaire to assess QoL of 194 patients with cervical lesions at Sichuan University West China Second Hospital between May 2010 and January 2011. Patients were surveyed before primary treatment and at 1, 3, and 6 months after primary treatment. Results showed a consistent decline in EQ-5D scores in the spectrum of cervical lesions at each time point after treatment (alP < 0.05). For patients with precursor lesions, there was an increasing trend along the timeline of treatment (P < 0.01). For patients with early-stage cervical cancer, EQ-5D scores declined in the first month (P = 0.01) and gradualy increased to higher levels at 6 months post-treatment than those before treatment (P < 0.01). EQ-5D scores followed a similar trend in patients with advanced cervical cancer (P = 0.04), though they did not statisticaly rebound after 6 months (0.84± 0.19 vs. 0.86± 0.11,P = 0.62). Regarding advanced cervical cancer, EQ-5D scores for women above 40 years of age appeared to recover more rapidly and reached higher levels than those for women below 40 years (P = 0.03). Caution and extra care are recommended in the early period of cervical cancer treatment given the slight deterioration in the QoL, and in particular, for younger cervical cancer patients. Our study implies that health care providers may need to improve the health-related QoL of cervical cancer patients.

  15. Clinical value of liquid-based thinPrep cytology test and cervical biopsy in the diagnosis of cervical Squamous epithelial lesions%液基薄层细胞学检测联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    赵瑞皎; 石曼丽; 张家兴; 孔令非

    2012-01-01

    exclude high grade intraepithelial lesions (ASC-H), 92 cases of low grade squamous intraepithelial lesions (LSIL), 80 cases of high grade squamous intraepithelial lesion (HSIL), and 1 5 cases of squamous cell carcinoma (SCC). There were 202 cases of cervical intraepithelial neoplasia (CIN)- 1, 22 cases of CIN-Ⅱ and CIN-Ⅲ, and 1 case of SCC among 1423 cases of ASC-US; and 12 cases of CIN- 1, 101 cases of CIN-Ⅱ and CIN-Ⅲ, and 5 cases of SCC among 214 cases of ASC-H. The consistent rates of diagnosis between TCT and colposcopy-directed biopsy were 63.04% (58/92) for LSIL, 81.25% (65/80) for HSIL, and 100% (15/15) for SCC, respectively. The consistent rates for SCC and HSIL were both higher than those for LSIL (P < 0.01). Conclusion: The high consistent rate of diagnose between TCT and colposcopy-directed biopsy suggests that the combination of these two diagnostic methods can improve the diagnostic rates of cervical cancer and precancerous lesions.

  16. p27蛋白在牙龈癌和癌前病变组织中的表达及临床病理学意义%The expression of p27 protein in gingival cancer and precancerous lesions tissues and its clinical pathological significance

    Institute of Scientific and Technical Information of China (English)

    闫威; 陈勇; 李浩博

    2013-01-01

    目的探讨细胞周期相关蛋白p27在牙龈癌及口腔黏膜癌前病变组织中的表达及其临床病理学意义。方法应用SP免疫组化法检测21例正常黏膜组织、26例癌前病变(白斑、红斑)组织及46例牙龈癌组织中p27蛋白的表达水平,分析p27蛋白与牙龈癌的病理分级和临床分期的关系。结果 p27蛋白在正常口腔黏膜组织与癌前病变及牙龈癌组织中的阳性率和阳性表达率差异有统计学意义(P <0构.05),在正常口腔黏膜组织中的表达高于另2组p27蛋白的表达与患者性别、年龄等因素无相关性( P >0.05),但与病变的临床分级及病理分期密切相关( P <0.05)。结论 p27蛋白与癌前病变和牙龈癌的发生发展有密切关系,并与牙龈癌的临床分期成反比关系,与牙龈癌的病理分级成正比关系。%Objective To investigate the expression of cell cycle related protein p 27 in gingival cancer and oral precancerous lesions tissues as well its clinicopathological significance .Methods The experimental levels of p27 protein were detected by immunohistochemistry ( SP) in 21 cases of normal mucous membrane tissues ,26 cases of oral precancerous lesions tissues ( leukoplakia , erythema ) and 46 cases of gingival cancer tissues .The correlation between p27 protein and pathological classification , clinical stage of gingival cancer was analyzed . Results There were significant differences in the positive rates and positive expression rates among normal mucous membrane tissues,precancerous lesions tissues and gingival cancer tissues ( P 0.05),however,which were closely correlated with clinical stage and pathological classification of gingival cancer ( P <0.05).Conclusion The p27 protein is closely related to the pathogenesis and development of precancerous lesions and gingival cancer , which is negatively correlated with clinical stage ,however ,which is positively related to pathological

  17. Preparation of rabbit buccal mucosa epithelial precancerous lesion model and observation of drug-reversed ultra-microstructure change in rabbit buccal epithelium%家兔颊黏膜上皮癌前病变模型制备及药物逆转超微结构观察

    Institute of Scientific and Technical Information of China (English)

    徐志彬; 吴明利; 高扬; 尔丽绵; 王士杰; 丛庆文

    2012-01-01

    Objective To establish the rabbit model of squamous epithelial precancerous lesion and observe the ultra-microstructure change of rabbit buccal mucosa epithelium in order to screen its revering drugs. Methods Sixty rabbits were divided into control group(n=12) and experimental group(n=48). Pathological histology examination and electron microscopy were performed for the buccal mucosa of rabbits in experimental group 16 weeks after a solution and a drug membrane containing the carcinogen of dimethyl-benzanthracene(DM6A)were sprayed or pasted on it. Thirty-two rabbits were randomly selected from experimental group to carry out drug-reversing experiments 12 weeks after a solution and a drug membrane containing the carcinogen of DMBA were sprayed or pasted on it. Results Sixteen weeks after the rabbits were exposed to DMBA, their buccal mucosa became rough and congested with thickened local leukoplakia and Ⅱ-Ⅲ atypical hyperplasia in 64.6%(31/48) of the rabbits in experimental group. Disarranged atypical hyperplasia cells and edematous mitochondria were found in nuclei. Twelve weeks after drug-reversing experiment, the progressive rate of precancerous lesion was lower in rabbits exposed to all-transretinoic acid(ATRA) than in controls. Although Kanlaite could reverse the precancerous lesion but no significant difference was observed between those exposed to it and controls. Conclusion Squamous epithelial precancerous lesion model can be induced by DMBA and effectively reversed with ATRA.%目的 建立鳞状上皮癌前病变动物模型,筛选癌前病变逆转药物,观察家兔颊黏膜上皮在此过程中超微结构变化.方法 12只家兔为空白对照组,48只家兔为实验组,将含有致癌剂二甲基苯并蒽(dimethyl-benzanthracene,DMBA)溶液及药膜涂抹或贴附于口腔颊黏膜;给药16周后做病理组织学检查及电镜扫描.再随机选取实验组中的32只进行药物逆转实验,12周后行病理组织

  18. 醋酸-靛胭脂染色在基层医院早期胃癌及癌前病变诊断中的应用%Application of acetic acid-indigo carmine dyeing in diagnosis of early gastric cancer and precancerous lesions in basic level hospital

    Institute of Scientific and Technical Information of China (English)

    王伟强; 李晓红; 宋国平; 贾长龙; 鹿志军; 李淼; 成伟丽

    2014-01-01

    Objective To explore the approaches to improve the detection of early gastric and precancerous lesions for basic level hospi-tals. Methods The 72 patients with abnormal gastric mucosa observed by gastroscope arranged with pathology after acetic acid-indigo car-mine dyeing were considered as the dyeing group, and 68 patients with abnormal gastric mucosa observed by gastroscope directly arranged with pathology were considered as the control group. The dyeing conditions of gastric mucosa were observed and compared to pathology detec-tion. The detection rate of early gastric cancer and precancerous lesions in the two groups were compared. Results After acetic acid-indigo carmine dyeing, there were 16. 7% of demonstrated discoloration, 63. 9% of poor dyeing, and 14. 3% of even dyeing. The detection rate of early gastric cancer and high grade intraepithelial neoplasia in patients with mucosa discoloration (91. 7%) was obviously higher than that in patients with poor dyeing (8. 6%) or even dyeing (0. 0%). The detection rate of low grade intraepithelial neoplasia or intestinal metaplasia in patients with poor dyeing (82. 6%) was obviously higher than that in patients with mucosa discoloration (8. 3%) or even dyeing (14. 3%). The detection rate of early gastric cancer and precancerous lesions in dyeing group (13. 9%,63. 9%) was obviously higher than that in control group (2. 9%,29. 4%). Conclusion The acid-indigo carmine dyeing could increase the diagnosis of early gastric cancer and precancerous lesions in basic level hospital. It is adaptable to extend approach in basic level hospital for its low cost and simple operation.%目的:探讨提高基层医院早期胃癌及癌前病变检出率的方法。方法经胃镜检查发现胃黏膜异常进行醋酸-靛胭脂染色后取活检病理检查患者72例为染色组,同期胃镜下发现黏膜异常患者直接取组织活检病理检查患者68例为对照组,观察胃黏膜染色情况并与病理

  19. Prevalence of high-risk human papillomavirus infection in different cervical lesion among organized health-examination women in Shanghai, China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Wen-ying; XUE Yue-zhen; CHEN Min; HAN Ling; LUO Man

    2008-01-01

    Background Cervical cancer is the second most common cause of death from cancer among women worldwide.Human papillomavirus(HPV)plays a central role in the etiology of cervical cancer.It is important to describe the prevalence of HPV infection in different types of cervical lesions and to explore the relation between HPV viral load and the severity of cervical lesions.Methods To describe the HPV infection prevalence and viral load in different age groups,we retrospectively investigated 6405 cases of women who were organized by their units to take health-examination.They were given Hybrid Capture ll tests between Janury 2005 and December 2006.The correlation between HPV viral load and pathology was assessed.Results Overall HPV infection prevalence was 29.1%(1864/6405),while in women 18-20 years old it was 54.4% (31/57),the highest among all age groups.After declining rapidly,HPV prevalence stabilized at about 30.0%in women aged 30 and older.Of the 6405 women,1 483 women had a colposcopic biopsy and 33.2%(492/1 483)were positive for HPV DNA.Twenty-one percent of women with a normal diagnosis(238/1 095)had HPV infection,a statistically significantly lower prevalence than in women with cervical lesions,including those with cervical intraepithelial neoplasia (68.8%in CINl,66.7% in CIN2,and 76.5%in CIN3)or with cervical cancer(94.1%).The correlation coefficient between viral load and cervical lesion severity was 0.134,which was not tatistically significant(P=0.075).Viral load values in women with CINs and cervical cancer were calculated,and no significant differences were dentified.Conclusions The revalence of high-risk HPV infection among women attending hospitals for health-examination in Shanghai is similar to the worldwide rate.HPV viral load can distinguish ervical lesions from normal individuals but cannot adequately predict the severity of cervical lesions.

  20. A pooled analysis of continued prophylactic efficacy of quadrivalent human papillomavirus (Types 6/11/16/18) vaccine against high-grade cervical and external genital lesions

    DEFF Research Database (Denmark)

    Kjaer, Susanne K; Sigurdsson, Kristján; Iversen, Ole-Erik;

    2009-01-01

    Quadrivalent human papillomavirus (HPV) vaccine has been shown to provide protection from HPV 6/11/16/18-related cervical, vaginal, and vulvar disease through 3 years. We provide an update on the efficacy of the quadrivalent HPV vaccine against high-grade cervical, vaginal, and vulvar lesions based...... trials (protocols 007, 013, and 015). Vaccine or placebo was given at baseline, month 2, and month 6. Pap testing was conducted at regular intervals. Cervical and anogenital swabs were collected for HPV DNA testing. Examination for the presence of vulvar and vaginal lesions was also done. Endpoints...... included high-grade cervical, vulvar, or vaginal lesions (CIN 2/3, VIN 2/3, or VaIN 2/3). Mean follow-up time was 42 months post dose 1. Vaccine efficacy against HPV 6/11/16/18-related high-grade cervical lesions in the per-protocol and intention-to-treat populations was 98.2% [95% confidence interval (95...

  1. Mapping of HPV transcripts in four human cervical lesions using RNAseq suggests quantitative rearrangements during carcinogenic progression.

    Science.gov (United States)

    Chen, Jinmiao; Xue, Yuezhen; Poidinger, Michael; Lim, Thimothy; Chew, Sung Hock; Pang, Chai Ling; Abastado, Jean-Pierre; Thierry, Françoise

    2014-08-01

    Two classes of Human papillomaviruses (HPV) infect the anogenital track: high risk viruses that are associated with risk of cervical cancer and low risk types that drive development of benign lesions, such as condylomas. In the present study, we established quantitative transcriptional maps of the viral genome in clinical lesions associated with high risk HPV16 or low risk HPV6b. Marked qualitative and quantitative changes in the HPV16 transcriptome were associated with progression from low to high grade lesions. Specific transcripts encoding essential regulatory proteins such as E7, E2, E1^E4 and E5 were identified. We also identified intrinsic differences between the HPV6b-associated condyloma transcript map and that of the HPV16-associated low grade CIN specifically regarding promoter usage. Characterization and quantification of HPV transcripts in patient samples thus establish the impact of viral transcriptional regulation on the status of HPV-associated lesions and may therefore help in defining new biologically-relevant prognosis markers. PMID:25092457

  2. 高危HPV病毒第2代杂交捕获法检测应用于宫颈癌以及癌前病变检查%Application of Hybrid Capture 2 for High-Risk Human Papillomavirus Testing in Cervical Cancer and Premalignant Lesion

    Institute of Scientific and Technical Information of China (English)

    张玉梅; 曹维克

    2011-01-01

    Objective: To explore the clinical value of hybrid capture 2 (hc2) in detection of high risk HPV in cervical cancer and pre-cancerous cervical lesions. Methods: Cervix exfoliated cells were detected with the second generation of hybrid capture hc2 method, and the diagnostic results such as specificity, sensitivity, accuracy, and pedicted value were compared with that of pathological diagnosis respectively. Results: The infection rate of HPV assayed by hc2 was 43. 8% in atypical squamous cell of undetermined signification (ASCUS), 7. 8% in ASCUS-H, 9. 7% low-grade squamous intraepithelial lesion(LSIL), and 28. 7% in high-grade squamous intraepithelial lesion (HSIL) respectively according to TBS diagnosis and classification system. For high grade-squ-mous intraepithelial lesion≥(CIN II ), the specificity, sensitivity, positive-predictive value and negative-predictive value by hc2 were 66. 7% , 93. 4% , 60. 0% , and 95. 0% respectively. In high virus load assay, there was significant difference between cervical cancer and CIN I (P<0. 01), and between cervical cancer/CIN and NILM group (P<0. 01), but there was no difference be-tween CIN and cervical cancer. Conclusion; The high-risk HPV test with hc2 is still a good method in diagnosing cervical cancer and premalignant lesion. Examining the high-risk HPV viral load with hc2 is related to cervical cancer and premalignant lesions, but not related to the severity of cervical lesions.%目的:探讨高危人类乳头瘤病毒(HPV)第二代杂交捕获实验(hc2)检测应用于宫颈癌以及癌前病变检查中的临床价值.方法:用hc2检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,对其特异度、敏感度、准确性、预测值等检测并分析.结果:hc2法检测高危型HPV的感染状况为无明确意义的非典型细胞的改变(ASCUS)44.7%,不典型鳞状细胞不除外高度病变(ASCUS-H)7.8%,低度鳞状上皮内病变(LSIL)19.7%,高

  3. Clinical Value of Endoscopic Esophageal Iodine Staining in the Early Diagnosis and Treatment of Esophageal Cancer and Precan-cerous Lesions%内镜下食管碘染在早期食管癌及癌前病变诊治中的临床应用价值

    Institute of Scientific and Technical Information of China (English)

    秦兴亚

    2015-01-01

    Objective To analyze the clinical value of endoscopic iodine dye in the early diagnosis and treatment of esophageal cancer and precancerous lesions .Methods Selecting 180 cases as the object , doing iodine endoscopic inspection process dyed checks on esopha-geal lesions in 50 cases, doing detailed records under the lesion morphology , location and size, and doing analysis and comparison on multi-ple biopsies and the esophageal squamous epithelium colored concrete results with the corresponding clinical and pathological examination . Results In 50 patients before receiving iodine dye check , 63 lesions points are suspected .In 40 cases after iodine staining dye -free area and lightly staining , a total of 58 points are found , which is about 1/3 of cases have ≥2 points with no staining area and lightly stained area . Conclusion Early diagnosis and treatment of esophageal cancer and precancerous lesions appear within the application in endoscopic has i -deal iodine dye detection results , with higher rate of clinical examination .So it is worth promotion and application .%目的:分析内镜下食管碘染在早期食管癌及癌前病变诊治中的临床应用价值。方法选取180例患者作为此次的研究对象,对50例经内镜检查有食管黏膜可能病变者给予碘染色检查,详细记录病灶的形态、位置以及大小等,行多处活检并把食管鳞状上皮组织具体的着色结果同相应的病理临床检查相互对比分析。结果50例患者接受碘染检查前怀疑病变点共63处,行碘染后40例出现无染色区以及淡染区共58处,约1/3病例出现≥2处无染色区以及淡染区。结论对早期食管癌以及癌前出现病变患者应用内镜下食管碘染色检查,可获得较为理想的检出效果,临床检诊率较高,值得在临床推广和应用。

  4. Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis

    Science.gov (United States)

    Wilting, Saskia M.; Verlaat, Wina; Jaspers, Annelieke; Makazaji, Nour A.; Agami, Reuven; Meijer, Chris J.L.M.; Snijders, Peter J.F.

    2013-01-01

    Deregulated expression of microRNAs (miRNAs) is common and biologically relevant in cervical carcinogenesis and appears only partly related to chromosomal changes. We recently identified 32 miRNAs showing decreased expression in high-grade cervical intraepithelial neoplasia (CIN) and carcinomas not associated with a chromosomal loss, 6 of which were located within a CpG island. This study aimed to investigate to what extent these miRNAs are subject to DNA methylation-mediated transcriptional repression in cervical carcinogenesis.   Methylation-specific PCR (MSP) analysis on a cell line panel representing different stages of human papillomavirus (HPV) induced transformation revealed an increase in methylation of hsa-miR-149, -203 and -375 with progression to malignancy, whereas expression of these miRNAs was restored upon treatment with a demethylating agent. All three miRNAs showed significantly increased levels of methylation in cervical carcinomas, whereas methylation levels of hsa-miR-203 and -375 were also significantly increased in high-grade CIN. A pilot analysis showed that increased hsa-miR-203 methylation was also detectable in HPV-positive cervical scrapes of women with high-grade CIN compared with controls. Similar to recent findings on hsa-miR-375, ectopic expression of hsa-miR-203 in cervical cancer cells decreased both the proliferation rate and anchorage independent growth. We found evidence for methylation-mediated transcriptional repression of hsa-miR-149, -203 and -375 in cervical cancer. Methylation of the latter two was already apparent in precancerous lesions and represent functionally relevant events in HPV-mediated transformation. Increased hsa-miR-203 methylation was detectable in scrapes of women with high-grade CIN, indicating that methylated miRNAs may provide putative markers to assess the presence of (pre)cancerous lesions. PMID:23324622

  5. Low NKp30, NKp46 and NKG2D expression and reduced cytotoxic activity on NK cells in cervical cancer and precursor lesions

    Directory of Open Access Journals (Sweden)

    Bravo-Cuellar Alejandro

    2009-06-01

    Full Text Available Abstract Background Persistent high risk HPV infection can lead to cervical cancer, the second most common malignant tumor in women worldwide. NK cells play a crucial role against tumors and virus-infected cells through a fine balance between activating and inhibitory receptors. Expression of triggering receptors NKp30, NKp44, NKp46 and NKG2D on NK cells correlates with cytolytic activity against tumor cells, but these receptors have not been studied in cervical cancer and precursor lesions. The aim of the present work was to study NKp30, NKp46, NKG2D, NKp80 and 2B4 expression in NK cells from patients with cervical cancer and precursor lesions, in the context of HPV infection. Methods NKp30, NKp46, NKG2D, NKp80 and 2B4 expression was analyzed by flow cytometry on NK cells from 59 patients with cervical cancer and squamous intraepithelial lesions. NK cell cytotoxicity was evaluated in a 4 hour CFSE/7-AAD flow cytometry assay. HPV types were identified by PCR assays. Results We report here for the first time that NK cell-activating receptors NKp30 and NKp46 are significantly down-regulated in cervical cancer and high grade squamous intraepithelial lesion (HGSIL patients. NCRs down-regulation correlated with low cytolytic activity, HPV-16 infection and clinical stage. NKG2D was also down-regulated in cervical cancer patients. Conclusion Our results suggest that NKp30, NKp46 and NKG2D down-regulation represent an evasion mechanism associated to low NK cell activity, HPV-16 infection and cervical cancer progression.

  6. MRI Findings of Cervical Spine Lesions among Symptomatic Patients and Their Risk Factors

    Directory of Open Access Journals (Sweden)

    J. Amir orang

    2003-06-01

    Full Text Available Background: Cervical spine and intervertebral discs are potentially prone to functional disorders. Objectives:This study sought type and distribution of different pathologies in the cervical spine and a possible relationship between the MRI findings and the probable risk factors of the degenerative disorders. Material and methods: This descriptive cross-sectional research was carried out from October 2000 to January 2002 in three referral centers in Tehran. All the patients had referred for cervical MRI for neck pain and/or radicular pain. Results: Totally 342 patients entered the study. Sixty percent of patients were male. The mean age was 55.1 12.1 years. Seventy-nine percent of patients had abnormal MRI findings (238 patients (70% had signs of degenerative processes and 31 patients (9% had the other findings with a total 308 pathologies. The most common findings were disc bulging/ protrusion (%21.1, disc dehydration (%20.1, disc herniation (%18.1, and canal stenosis(%17.5. Older age, male gender and history of neck trauma were associated with increasing probability of degenerative changes (P-Values<0.05. Conclusion: Types of cervical spine pathologies are comparable to other reports. The anatomical distribution of disc bulging and protrusion in our study are similar to other reports. Likewise age, gender and a history of trauma to the neck were closely associated with the degenerative signs on the MR images.

  7. Quadrivalent HPV vaccine effectiveness against high‐grade cervical lesions by age at vaccination: A population‐based study

    Science.gov (United States)

    Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim‐Dahlström, Lisen

    2016-01-01

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006–2013 and aged 13–29 (n = 1,333,691) was followed for vaccination and histologically confirmed high‐grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1‐IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18–0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46–0.64) and 22% (IRR = 0.78, 95%CI = 0.65–0.93) for those initiating vaccination at ages 17–19, and at ages 20–29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long‐term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer. PMID:26856527

  8. Cervical Cytology Smear Combined Colposcopy in the Diagnosis of Cervical Lesions in Clinical Observa-tion%宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床观察

    Institute of Scientific and Technical Information of China (English)

    许盛芳

    2015-01-01

    Objective To explore the cervical cytological smear the clinical effect of combined colposcopy in the di-agnosis of cervical lesions.Methods Between January 2013 and December 2013 in our hospital to accept the cervical cytological smear combined colposcope examination of 880 cases of married women as the research object, on the cervical lesions of inspection, analysis of the clinical condition.Results Of cervical smear cytologic examination and vaginoscope diagnosis coincidence rate in RCI score photogenic compared with significant difference ( P<0.05) , after statistics treat-ment, under colposcopy in the diagnosis of CIN sensitivity was 94.84%, specificity is 88.81%, both the positive pre-dictive value was 72.31% and negative predictive value was 95.63%.Conclusions Cervical cytological smear com-bined colposcope examination before screening of cervical lesions can ensure cerical cancer lesions and early diagnosis of cervical cancer, can also become local screening test for cervical cancer lesion before effective policies.%目的:探讨宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床效果。方法选取2013年1月~2013年12月在我院接受宫颈细胞学涂片联合阴道镜检查的880例已婚妇女作为研究对象,对其进行宫颈病变的检查,分析其临床情况。结果宫颈涂片细胞学检查与阴道镜下RCI评分诊断在符合率上相比较差异具有显著性( P<0.05),经统计学处理后,阴道镜下诊断CIN的敏感性是94.84%,特异性是88.81%,两者联合的阳性预测值是72.31%,阴性预测值是95.63%。结论宫颈细胞学涂片联合阴道镜检查筛选子宫颈病变可以保证宫颈癌前病变和前期宫颈癌的确诊,还可以成为当地筛选检查宫颈癌前病变的有效政策。

  9. 内镜窄带成像技术在诊断早期喉癌及其癌前病变中的作用研究%The value of narrow band imaging in the diagnosis of early laryngeal cancer and the precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    于红; 高圣锐; 孙志; 祝威

    2015-01-01

    目的:探讨窄带成像技术(narrow band imaging,NBI)在内镜诊断早期咽喉恶性肿瘤及癌前病变中的临床应用价值。方法选取2013年6月至2014年3月因主观症状就诊于我院门诊,经电子喉镜检查发现咽喉粘膜异常增生及喉肿物疑似恶性病变的104例患者,分别在白光和 NBI 模式下观察咽喉部黏膜,记录病变的大小、范围,同时进行 NBI 分级,并对病灶性质做出预判,对 NBI 模式下发现的117个病灶均取活检,以病理结果作为诊断金标准,将其他检查结果与之作对照。结果1.窄带成像技术在对病损轮廓、黏膜表面及粘膜下微血管形态显示方面均明显优于普通白光内镜图像,差异有显著性(P <0.05)。2.104例怀疑喉癌或癌前病变的患者中共发现117个病灶,其中白光模式下病变检出率为89.7%,应用 NBI 模式检出率为99.1%。NBI 内镜对咽喉恶性肿瘤及癌前病变诊断的灵敏度为91.4%,而普通白光内镜的灵敏度为74.1%,二者之间的差异有统计学意义。3.NBI 分级与病理诊断的关系:声带息肉等良性病变主要表现为 NBI Ⅰ级(10/12),慢性喉炎等炎性病变主要表现为 NBI Ⅱ级(15/19),轻度不典型增生主要表现为 NBI Ⅲ级(10/15),重度不典型增生主要表现为 NBI Ⅳ级(5/7),重度不典型增生及恶性肿瘤主要表现为 NBI Ⅴ级(52/64)。结论与白光模式相比,NBI 模式能清晰地显现病变的轮廓及黏膜表层及黏膜下层微血管的各种形态变化,可增强病变的可识别性。内镜窄带成像技术操作简便、安全,较白光模式更易发现细微变化的病灶,亦可使活检阳性率明显提高,在早期咽喉恶性肿瘤及癌前病变的诊断有一定的临床应用价值。%Objective To assess the value of narrow band imaging (NBI)in the diagnosis of precancerous lesions and laryngeal

  10. Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy Ex Vivo

    Directory of Open Access Journals (Sweden)

    Ling Yang

    2011-01-01

    Full Text Available Confocal light absorption and scattering spectroscopic (CLASS microscopy can detect changes in biochemicals and the morphology of cells. It is therefore used to detect high-grade cervical squamous intraepithelial lesion (HSIL cells in the diagnosis of premalignant cervical lesions. Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low-grade cervical squamous intraepithelial lesion (LSIL. The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non-HSIL cells. Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000 nm. Between 600 nm to 800 nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non-HSIL. Relative intensity peaks occurred at 700 nm and 800 nm. CLASS sensitivity and specificity results for HSIL and non-HSIL compared to cytology diagnoses were 80% and 90%, respectively. This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions. Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.

  11. Clinical Value of Endoscopic Multi-band Mucosectomy in Treatment of Early Esophageal Cancer and Precancerous Lesions%内镜下多环黏膜切除术在治疗早期食管癌及癌前病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    刘晓波; 高子夜; 金曙; 李胜保; 童强; 王强

    2014-01-01

    Objective To investigate the feasibility,safety and efficacy of endoscopic multi-band mucosectomy (EMBM) in the diagnosis and treatment of early esophageal cancer and precancerous lesions.Methods A total of 33 patients with early esophageal cancer and precancerous lesions were confirmed by endoscopy,ultrasound endoscopy and pathology.The lesion extent was confirmed and observed by using Lugol staining before the EMBM was preformed under general anesthesia.Meanwhile,the specimens from the patients were sent for pathological examination.After the operation,all patients were regularly rechecked by gastroscope,and clinical effect in patients were also evaluated.Results Thirty-five lesions in 33 patients were successfully removed,the rate of complete resection was 100%.The pathological characteristic in 33 patients showed 2 cases of slightly atypical hyperplasia,8 cases of moderately atypical hyperplasia,16 cases of severe atypical hyperplasia,and 7 cases of early esophageal squamous cell carcinoma.The coincidence rate of pathological examination was 81.8% (27/33).Seven complications in 33 patients were observed,in which 2 cases underwent endoscopic hemostasis.All cases did not show the complications such as perforation,obstruction and infection.Wound exudation were not observed 2 weeks after surgery,the wound were completely healed and scar formation have been observed 4 weeks after the operation,and recurrence was not observed in all cases after 6 to 8 months follow-up.Conclusion EMBM is worthy of popularization for treating early esophageal cancer and precancerous lesions because of its advantages of easy operation,high safety,exact curative effect and less complications.%目的:探讨内镜下多环黏膜切除术(endoscopic multi-band mucosectomy,EMBM)在早期食管癌及癌前病变治疗中的可行性、安全性及有效性.方法:33例经普通胃镜、超声胃镜及病理学检查明确诊断为早期食管癌及癌前病变患者,术前行Lugol碘

  12. 靛胭脂联合美蓝染色在早期胃癌及癌前病变诊断中的价值%Value of Combined Staining with Indigo Carmine and Methylene Blue in Diagnosis of Early Gastric Cancer and Precancerous Lesions

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    朱国福; 朱学鹰; 祝三秀

    2015-01-01

    目的:探讨靛胭脂联合美蓝染色在诊断早期胃癌及癌前病变中的临床应用价值。方法将300例内镜下胃黏膜异常表现患者随机分为2组:染色组150例内镜下先喷洒0.2%靛胭脂染色,发现可疑病灶后,再喷洒0.4%美蓝染色,然后在染色异常部位给予活检并送病理组织学检查;对照组150例不作染色,按肉眼判断,常规活检。结果染色组150例患者中胃黏膜有肠上皮化生38例、轻-中度不典型增生50例、重度不典型增生9例、早期胃癌8例,对照组150例患者中肠上皮化生29例、轻-中度不典型增生19例、重度不典型增生2 例。染色组早期胃癌及癌前病变总检出率显著高于对照组(70.0%比33.3%,P <0.005)。结论靛胭脂联合美蓝染色能够指导内镜下上消化道病变的活检,能有效提高早期胃癌和癌前病变的检出率。%Objective To investigate the clinical value of combined staining with indigo carmine and methylene blue in the diagnosis of early gastric cancer and precancerous lesions.Methods A total of 300 patients with endoscopic abnormalities of gastric mucosa were randomly divided into two groups,with 150 patients in each group.In staining group,0.4% methylene blue was sprayed after suspicious lesions were observed by endoscopic staining with 0.2% indigo carmine,and then biopsy at abnormal staining sites was performed for histopathologic examination.In control group,patients only received naked eye examination and routine biopsy.Results Among the 150 patients in staining group,38 had gastric intestinal metaplasia,50 had mild to moderate atypical hyperplasia,9 had severe atypical hyperplasia,and 8 had early gastric cancer.Among the 150 pa-tients in control group,29 had gastric intestinal metaplasia,19 had mild to moderate atypical hy-perplasia,and 2 had severe atypical hyperplasia.The detection rate of early gastric cancer and pre-cancerous lesions in staining

  13. Clinical Application of Colposcopy Cervical Biopsy and Liquid-based Cytology Test in the Screening of Cervical Lesions%阴道镜宫颈活检与 TCT 联合检测在宫颈病变中的应用

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    冷海娜; 孙名芳

    2013-01-01

    Objective To explore the clinical application value of colposcopy cervical biopsy and liquid -based cytology test in the screening of cervical lesions .Methods Four hundred and eighty-three patients who udergone the TCT examination from January to December in gynecological clinic were chosen for pathological colposcopy cervical biopsy To select 483 gynecological clinic patients who had the TCT examination and were inspected to be abnormal in Care Hospital from January to December ,2012 ,have pathological colposcopy cervical biopsy for them and retrospective a-nalysis fwas carried out .or their entire clinical datum .Among which the TBS diagnostic system wasis used in the TCT in-spection report ,which contributed to the diagnosis ,as well as combined with the pathological examination report to make the diagnosis.Results Among the patients with the TCT result being ASC-US351,CINⅠ~CINⅢ223 patients(63. 53%) were detected with the pathological colposcopy cervical biopsy;CINⅠ~CINⅢ75 patients(91.46%) were de-tected with the pathological colposcopy cervical biopsy;CINⅠ~CIMⅢ41 patients were detected with the pathological colposcopy cervical biopsy and among the 6 SCC patients ,SCC 6 patients were detected with the colposcopy cervical bi-opsy .Conclusion The diagnose accordance rate of TCT examination and cervical biopsy diagnosis is high ;the diagnose accordance rate of cervical cancer with CIN of higher level is high .Precancerous lesions of uterine cervix can be found at an earlier age with the adoption of colposcopic cervical biopsy combined with TCT examination and stopped or slowed down ,which is the safe ,reliable and accurate examination method for the screening of cervical cancer .%目的:探讨阴道镜宫颈活检与宫颈薄层液基细胞学( TCT )在宫颈病变筛查中的临床应用价值。方法选取2012年1月~12月在妇科门诊行宫颈薄层液基细胞学( TCT)检查异常的483例患者,并同时行阴道镜宫颈活检

  14. Restoration of non-carious cervical lesions with ceramic inlays: A possible model for clinical testing of adhesive cements

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    Michal Staninec

    2012-01-01

    Full Text Available Introduction: There are many luting cements coming to market which claim to be adhesive, but there is no clinical protocol currently for testing these claims. There is a standardized protocol for testing direct restorations bonded to dentin and it is used extensively. Case Report: We describe a clinical procedure for restoring a non-carious cervical lesion (NCCL with a ceramic inlay using Computer-Aided Design and Computer-Aided Manufacturing (CAD-CAM technology and an adhesive resin cement.The procedure was straightforward and the result was good at one month. Discussion: NCCL′s can be restored with CAD-CAM technology in one appointment. This technique can be used to clinically test adhesion of luting cements to dentin, similarly to the current standard for direct restorations.

  15. The Distribution and Infection of HPV in Cervical Biopsy Lesions among 450 Women%450例宫颈活检组织中人乳头瘤病毒的分布特征

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    王翔; 钱福初; 顾栋桦; 秦基取; 陈瑜; 李雄峰

    2011-01-01

    [Objective] To determine the distribution and infection of HPV in cervical cancer and precancerous lesions. [Methods]Four hundred patients with abnormal cytology were examined by colposcopy. HPV diagnosis was done by polymerase chain reaction using MY09/MY11 primers, for genotyping blot hybridization was used. The pathological results confirmed standards. [Result]A total of 294 women were HPV-DNA positive. HPV-16 was found in 36.7% of all women with positive HPV-DNA,HPV58 in 22. 1%, HPV-18 in 18.7% and HPV-52 in 16. 0%. The infection of HPV in lesions with cervicitis, CIN Ⅰ , CIN Ⅱ and CIN Ⅲ were 55.6 %, 69 %, 82.7 % and 88.9%, respectively. HPV-16,-58,-18 and -52 were found in different cervical lesions. [Conclusions] The finding of HPV-58 and -52 expects HPV-16 and -18 among Huzhou women with CIN Ⅱ and CIN Ⅲ , and has important implications for the development of type-common HPV vaccines.%[目的]了解阴道镜检查患者的宫颈活检组织中HPV的感染率和亚型分布.[方法]对宫颈脱落细胞学异常的患者450例进行阴道镜下定位活检.活检组织同时行HPV基因分型和病理诊断,HPV基因分型采用斑点杂交方法进行.[结果]在450例宫颈活检患者中,其HPV感染率为65.3%.在HPV-DNA阳性患者中,检出率最高的HPV-16占36.7%,依次HPV-58为22.1%,HPV-18为18.7%,HPV-52为16.0%.在宫颈炎、CIN Ⅰ、CIN 、Ⅱ、D,CIN Ⅲ中HPV-DNA阳性率分别为55.6%、69%、82.7%和88.9%.在宫颈炎、CIN Ⅰ、CIN Ⅱ、CIN Ⅲ组织中均检测到HPV-16、-58、-18和-52.[结论]本研究中的宫颈活检组织中不仅检测到HPV-16和-18,还检测到HPV-58和-52感染,后者的发现为开发宫颈癌多价复合型疫苗提供了事实依据.

  16. ANÁLISIS COMPUTACIONAL DE LESIONES CERVICALES NO CARIOSAS EN UN PREMOLAR SUPERIOR COMPUTER ANALYSIS OF NON-CAVITY CERVICAL LESIONS IN AN UPPER PREMOLAR

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    Patricio Cendoya

    2007-08-01

    Full Text Available Se presenta un modelo tridimensional de elementos finitos para investigar el efecto producido por las fuerzas de oclusión en la distribución y magnitud del campo tensional sobre un premolar superior. De esta forma, se busca definir cuál es la situación de carga de oclusión más crítica desde el punto de vista tensional sobre el esmalte dental en la zona cervical que pudiese dar origen a una lesión del tipo no cariosa. Utilizando elementos finitos hexaédricos lineales y procesando el modelo con el programa SAMCEF se realiza un análisis numérico estático lineal considerando que el premolar es isótropo y homogéneo. Se definen siete estados de carga asociados a una fuerza de oclusión de 170 N para las cuales se investiga la distribución y magnitud de los campos tensionales sobre el premolar. Los resultados numéricos permiten concluir que las tensiones máximas sobre el premolar tienden a concentrarse en la zona cervical alcanzando sus valores máximos cuando la carga de 170 N actúa de forma inclinada a 45º con respecto a la vertical. La relevancia de la presente investigación radica en el hecho que numéricamente se demuestra que la distribución y zona en donde los campos tensionales tienden a concentrase y alcanzar sus valores máximos son coincidentes con las zonas del diente en donde clínicamente se observan lesiones no cariosas.A three-dimensional model of finite elements in order to investigate the effect produced by occlusion forces on both distribution and magnitude of the tensional field on an upper premolar is hereby presented. Thus, defining from a tensional point of view, which is the situation of the most critical occlusion loading on dental enamel capable to produce a non-cavity lesion is intended. By using finite linear hexahedral elements and processing the models through SAMCEF software, a static linear numerical analysis is performed, considering that premolar is isotropic and homogeneous. Seven load states

  17. 莪术油对大鼠乳腺癌癌前病变组织中VEGF mRNA表达的影响%Zedoary turmeric oil effect on rats with precancerous lesions of breast carcinoma in VEGF mRNA expression

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    宋爱莉; 许振国

    2012-01-01

    Objective: Through observing the influence of zedoary turmeric oil on rats with precancerous lesions of breast carcinoma tissue in vascular endothelial growth factor ( VEGF ) and its mRNA expression, discuss the mechanism of Zedoary turmeric oil treating preeancerous lesions of breast cancer- Method: 275 SD rats were randomly divided into control group, model group, the TAM group, injection group and zedoary turmeric oil, in small, high dose group,then use DMBA induced the model of precancerous lesions of breast cancer. After intervention treatment for 4 weeks, the animals were sacrificed in 4 batches after 8-14 weeks, using insitu method for the determination of specimens of VEGF mRNA expression. Result; Each lest group of different types of breast tissue in VEGF mRNA expression positive rate and positive cells positive rate increased, A typical hyperplasia of breast tissue,The VEGF mRNA expression positive rate in the intervention group were significantly lower than those in model group,and the intervention group VEGF mRNA positive cells positive rate were significantly lower than those in model group, Zedoary turmeric oil group is lower than injection and TAM group ,In the atypical hyperplasia specimens of VEGF mKNA expression, VEGF mRNA positive cells positive rate of Zedoary turmeric oil the in the large dose group was significantly lower than small dose group. Conclusion: Zedoary turmeric oil can effectively reduce DMBA induced rat mammary carcinoma precancerous lesion tissue VEGFmRNA expression intensity and Inhibit angiogenesis, so May he the effective mechanism of blocking in breast cancer.%目的:通过观察莪术油对大鼠乳腺癌癌前病变组织中血管内皮生长因子(VEGF)及其mRNA表达的影响,探讨莪术油治疗乳腺癌前病变的机理.方法:275只SD大鼠随机分为空白对照组、疾病模型组、三苯氧胺组、康莱特组及莪术油小、中、大剂量组.采用DMBA诱导乳腺癌癌前病变造模,干预治疗4

  18. Clinical impact of de-regulated Notch-1 and Notch-3 in the development and progression of HPV-associated different histological subtypes of precancerous and cancerous lesions of human uterine cervix.

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    Richa Tripathi

    Full Text Available BACKGROUND: Cervical cancer is the leading cause of cancer related deaths among women in India. Limited reports are available for Notch-1 and Notch-3 protein in cervical carcinoma, which play crucial role in cell proliferation, differentiation, and apoptosis. METHODS: This study was designed to evaluate the role of Notch-1 and Notch-3 with context to HPV infection in cervical carcinoma. A total of 168 tissue biopsy samples comprising of tumor specimens (n = 98, precancer (n = 30 and non-neoplastic cervical tissues (n = 40 were screened for HPV infection by PCR and expression of Notch-1 and Notch-3 protein by Immunohistochemistry and Immunoblotting. RESULTS: 80% (24/30 were found to be positive for HPV in precancer and 86.7% (85/98 in cancer patients. Notch-1 expression of precancer and cancer cases was found to be significantly down-regulated with severity of disease in nuclear (3.43±0.29; 2.04±0.19, p = 0.0001, p = 0.0001 and cytoplasm (3.07±0.29; 2.29±0.17, p = 0.0001, p = 0.0001 obtained from different stages as compared to normal cervix tissue (5.40±0.19, 4.97±0.15; p<0.001; p<0.001. However, Notch-3 expression of above cases was significantly up-regulated with severity of disease and showed intense nuclear (4.17±0.39; 4.74±0.18, p = 0.0001, p = 0.0001 and cytoplasm (3.67±0.36; 4.48±0.18, p = 0.0001, p = 0.0001 of different stages as compared to normal cervix tissue (0.95±0.20, 0.70±0.20; p<0.001; p<0.001 respectively. CONCLUSIONS: These findings suggest that Notch-1 and Notch-3 may play an important role with synergistic effect of HPV in regulating development and proliferation of cervical cancer through the deregulation of Notch signalling. This study also shows the clinical utility of both proteins which may be used as predictable biomarkers in diagnosing different histological sub-types of HPV associated cervical cancer. Nevertheless, abnormal activation of this pathway may provide

  19. The significance of HPV genotyping in cervical lesions%宫颈组织病变与检测HPV基因分型的意义

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    张达衡; 陈红玲; 杨春媚; 陈瑞林

    2013-01-01

    目的 了解宫颈病变患者人乳头瘤病毒HPV感染率及基因型分布情况.探讨人乳头瘤病毒基因分型与宫颈组织病变的相关性,为临床宫颈癌的筛查、防治提供参考.方法 收集在本院诊治2 000例疑似宫颈病变女性,应用基因芯片技术检测23种HPV基因型,统计HPV各基因型的感染率,分析HPV及基因型分布与子宫病变的关系.结果 2000例宫颈疑似宫颈病变女性中,基因芯片检测出的HPV阳性标本662例,HPV感染率为33.1%(662/2 000).354例宫颈不同程度病变组织中HPV阳性295例,其中LSIL,HSIL及宫颈癌样本中HPV的检出率分别为76.6%(157/205)、91.9%(125/136)、100%(13/13).共检出12种HPV基因型,检出率最高的5种基因型分别为HPV16,18,58,31,52.宫颈病变级别越高,HPV多重感染率越高.结论 HPV亚型感染与宫颈组织不同程度病变密切相关,HPV基因分型检测及诊断在宫颈病变及宫颈癌筛查及防治过程中具有重要意义.%Objective To investigate the infection rate of human papillomavirus (HPV) and distribution of HPV genotypes in cervical lesions,and discuss the correlationbetween HPV genotypes and cervical lesions to provide a reference for cervical cancer screening,prevention and treatment.Methods 2 000 cases of women with suspected cervical lesions were detected by 23 cases of HPV genotyping based on gene chip technology,and infection rates of HPV genotypes and the correlation between HPV genotypes and cervical lesions were analyzed.Results 662 cases were HPV positive in 2 000 cases of women with suspected cervical lesions,with the HPV infection rate of 33.1 % (662/2 000),and 295 cases were HPV positive in 354 cases of women with cervical lesions.For LSIL,HSIL and cervical cancer,HPV detection rates were 76.6% (157/205),91.9% (125/136),100% (13 / 13),respectively.12 kinds of HPV genotypes were detected,and the top five genotypes of the highest detection rate were HPV16,18,58,31,52.The higher

  20. Study on the clinical treatment of low - grade squamous intraepithelial lesion diagnosed by cytological test%宫颈细胞学诊断低度上皮内瘤变的临床处理探讨

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    张娟; 蔡林儿; 严杏

    2012-01-01

    目的:探讨宫颈细胞学诊断低度上皮内瘤变的意义及临床处理方法.方法:对2010年5月~2011年11月在广东省中医院就诊并经TCT检查为LSIL的195例女性病例进一步行HPV-DNA-PCR检测及阴道镜检查,观察其最终病理结果的构成及在不同年龄阶段的分布差异.结果:在195例LSIL患者中,高危HPV阳性144例(73.8%),病理诊断为癌前病变及癌者共87例(60.4%);高危HPV阴性51例(26.2%),病理诊断为癌前病变及癌者共8例(15.7%).在35岁以下的93例中,病理诊断为癌前病变及癌者共43例(46.2%);在35~45岁的69例中,病理诊断为癌前病变及癌者共41例(69.9%);45岁以上33例中,病理诊断为癌前病变及癌者共11例(33.3%).结论:在TCT诊断为LSIL的患者中,高危HPV阳性与高危HPV阴性患者的宫颈病变检出率差异有统计学意义,不同年龄段之间宫颈病变检出率差异有统计学意义,临床处理方法及随访计划的制定与年龄及HPV感染情况有关.%Objective: To explore the significance of cervical cytological test for diagnosis of low - grade squamous intraepithelial lesion (LSIL) and the clinical treatment. Methods: A total of 195 female cases who were diagnosed as low grade squamous intraepithelial lesion (LSIL) by TCT in the hospital from May 2010 to November 2011 further underwent HPV - DNA PCR and colposcopy, the proportion of finally pathological results and the difference of different age distributions were observed. Results: Among 195 patients with LSIL, 144 patients (73. 8% ) were found with positive high risk HPV, 87 patients (60. 4% ) were diagnosed as cervical precancerous lesion and cervical cancer by pathological diagnosis; 51 patients (26. 2% ) were found with negative high risk HPV, 8 patients (15. 7% ) were diagnosed as cervical precancerous lesion and cervical cancer by pathological diagnosis. Among 93 patients under 35 years old, 43 patients (46.2% ) were diagnosed as cervical precancerous

  1. Effect Observation on HPV, TCT and Vaginal Examination in Cervical Le-sions and Cervical Screening%宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果探讨

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    金伟蓉; 孔彩霞

    2015-01-01

    目的:探讨宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果。方法整群选取2013年5月—2015年5月于该院接受TCT及HPV检查的妇女1280例。对这些患者再进行阴道镜检查以及宫颈组织活检,然后将检验的结果与病理结果进行统计学的分析比对。结果 TCT检查的阳性检出率为9.61%(123/1280);HPV检查的阳性检出率为12.03%(154/1280)。结论临床上对宫颈病变结合筛查可联合CTC、HPV以及阴道镜检查,这样可提高宫颈病变的检出率,减少漏诊,对临床的及时治疗提供极大的帮助。%Objective To observe the effect of HPV, TCT and vaginal examination in cervical lesions and cervical screen-ing. Methods 1280 cases of women who received TCT and HPV examination in our hospital from May 2013 to May 2015 were selected and were given vaginal examination and cervical biopsy, the test results and the pathological results were compared and analyzed statistically. Results The positive detection rate of TCT was 9.61% (123/1280)and 12.03%(154/1280)of HPV. Conclusion HPV, TCT and vaginal examination in treatment of cervical lesions and cervical screening can improve the detection rate of cervical lesions, reduce missed diagnosis and offer great help to timely treatment clinically.

  2. Diagnostic aids for detection of oral precancerous conditions

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    Diana V Messadi

    2013-01-01

    Oral cancer has a tendency to be detected at late stage which is detrimental to the patients because of its high mortality and morbidity rates. Early detection of oral cancer is therefore important to reduce the burden of this devastating disease. In this review article, the most common oral precancerous lesions are discussed and the importance of early diagnosis is emphasized. In addition, the most common non-invasive oral cancer devices that can aid the general practitioners in early diagnosis are also discussed.

  3. Priority Setting for Improvement of Cervical Cancer Prevention in Iran

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    Azam Majidi

    2016-04-01

    Full Text Available Background Cervical cancer is the fourth most common cancer among women worldwide. Organized cervical screening and vaccination against human papilloma virus (HPV have been successful interventions for prevention of invasive cervical cancer (ICC. Because of cultural and religious considerations, ICC has low incidence in Iran and many other Muslim countries. There is no organized cervical screening in these countries. Therefore, ICC is usually diagnosed in advanced stages with poor prognosis in these countries. We performed a priority setting exercise and suggested priorities for prevention of ICC in this setting. Methods We invited experts and researchers to a workshop and asked them to list important suggestions for ICC prevention in Iran. After merging similar items and removing the duplicates, we asked the experts to rank the list of suggested items. We used a strategy grid and Go-zone analysis to determine final list of priorities for ICC prevention in Iran. Results From 26 final items suggested as priorities for prevention of ICC, the most important priorities were developing national guidelines for cervical screening and quality control protocol for patient follow-up and management of precancerous lesions. In addition, we emphasized considering insurance coverage for cervical screening, public awareness, and research priorities, and establishment of a cervical screening registry. Conclusion A comprehensive approach and implementation of organized cervical screening program is necessary for prevention of ICC in Iran and other low incidence Muslim countries. Because of high cost for vaccination and low incidence of cervical cancer, we do not recommend HPV vaccination for the time being in Iran.

  4. Gene reversal mechanism of Weifuchun combined with colloidal bismuth pectin on patients with precancerous lesion of gastric carcinoma%胃复春联合胶体果胶铋对胃癌前病变基因的逆转性研究

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    郑波; 王建嶂; 裴继华; 李国刚; 薛战雄

    2016-01-01

    目的:观察胃复春联合胶体果胶铋胶囊对胃癌前病变患者Notch1、β-链蛋白(β-catenin )、人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)的影响。方法115例胃癌前病变患者随机分为试验组60例,对照组55例。试验组口服胃复春1.44 g,胶体果胶铋150 mg,每天3次;对照组口服奥美拉唑20 mg,每天2次,口服硫糖铝0.25 g,每天3次,2组均连续治疗4周。观察2组患者治疗前后Notch1、β-catenin及PTEN的表达。结果治疗后,试验组的总有效率(68.3%)显著高于对照组(43.6%, P<0.01)。治疗后,试验组Notch1、β-catenin阳性率与治疗前相比均有所下降,且试验组比对照组下降更显著( P<0.01)。治疗后试验组PTEN阳性表达显著提升,且优于对照组( P<0.05)。结论胃复春联合胶体果胶铋胶囊不仅能够降低胃癌前病变中Notch1、β-catenin表达,提升PTEN表达,同时对提高治疗效果和治疗安全性也具有显著的效果。%Objective To discuss the effect of Weifuchun combined with colloidal bismuth pectin capsules on Notch 1, β-catenin and phosphatase and tensin homolog deleted on chromosome ten ( PTEN) in patients with precancerous lesion of gastric carcinoma.Methods A total of 115 patients with precancerous lesion of gastric carcinoma were randomly divided into experiment group ( n =60 ) and control group (n=55).Patients in experiment group were given Weifuchun 1.44 g, colloidal bismuth pectin capsules 150 mg, 3 times a day, while patients in control group given omeprazole 20 mg, 2 times a day, and sucralfate 0.25 g, 3 times a day, all patients treated for 4 weeks.The expressions of Notch1, β-catenin and PTEN of the two groups were observed.Results After treatment , the total effective rate of experiment group (68.3%) was significantly higher than that of control group (43.6%, P<0.01 ).After treatment , the positive rates of Notch 1

  5. Risk assessment on liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in the diagnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    Ling-Zhi Chen

    2016-01-01

    Objective: To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression difference and significance of human papilloma virus L1(HPV L1) protein in exfoliative cells of different cervical lesions. Methods: A total of 1 400 cases of patients in gynaecological clinics of our hospital from January 2009 to August 2013 were selected and were given liquid based cytology test(LCT) examination and HPV L1 protein expression detection in exfoliative cells. Meanwhile, cervical biopsy pathology results were taken as baseline for make a comparison. Results: (1) In such1400 cases of women: LCT(+)-30 cases, accounting for 2.1%, HPV L1 protein detection(+)-18 cases, accounting for 1.29%; LCT and HPV L1 protein detection(+)-8 cases, LCT and HPV L1 protein detection(-)- 1360 cases. (2)Pathological tissue biopsy was performed on 30 cases of patients with LCT(+), results showed that(+)were 23 cases; pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that (+)were 14 cases. Among 1 360 cases with LCT and HPV L1 protein detection(-), 497 cases were willing to accept the pathological biopsy, and the results showed that 1 case presented(+), accounting for 0.2%. Total 545 cases were willing to accept the pathological biopsy, among whom, 38 cases presented(+), accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection, and difference had statistical significance. Conclusion: liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical lesions, and were expected as the effective method in the early clinical screening of cervical neoplasia lesions.

  6. Expression of Caspase-3 in gastric tissue of rats with precancerous lesions treated by different traditional Chinese herbs%中药不同组方对胃癌前病变模型大鼠胃黏膜细胞Caspase-3表达的影响

    Institute of Scientific and Technical Information of China (English)

    李岩; 王垂杰

    2012-01-01

    [Objective]To explore the effects of different traditional Chinese herbs on gastritic morphological and histological changes in rats with chronic atrophic gastritis with dysplasia. [Methods] The animal models of chronic atrophic gastritis with precancerous lesions were made by using chemical substances,and then devided into different groups for therapy. The expression of Caspase-3 in gastric tissue was detected by microplate reader for enzyme activity. [Results]Either in prevention or treatment groups, the expressions of Caspase-3 were significantly increased by the intervention of TCM and western medicine, P < 0. 05 when compared with the model group. Jianpi Huayu Decoction group was better than other groups. [Conclusion] TCM with the function of strengthening spleen-qi and smoothing blood stagnationCJianpi Huayu) strengthening spleen-qi(Jianpi) ,and smoothing blood stagnationC Huayu) played an important role in the prevention and treatment of the gastric epithelial dysplasia. The mechanism might be contributed to the increase of the Caspase-3 expression in gastric tissue. TCM with the function of Jianpi Huayu were better than the other two treating principles(Jianpi and Huayu) in the treatment of atrophic gastritis with precancerous lesions.%[目的]探讨不同组方中药对慢性萎缩性胃炎伴不典型增生大鼠胃黏膜形态、组织学改变的影响.[方法]采用化学药物刺激的方法复制慢性萎缩性胃炎癌前病变动物模型并分组用药物干预.用酶标仪检测各组大鼠胃组织中Caspase-3表达的变化.[结果]无论是预防组还是治疗组,中药、西药的干预均可以使Caspase-3的表达增加,与模型大鼠比较差异有统计学意义(P<0.05);健脾化瘀方要优于其他药物.[结论]各组中药均可以通过增加胃组织中Caspase-3的表达达到对胃癌前病变的治疗作用,其中健脾化瘀方治疗萎缩性胃炎癌前病变的作用要明显优于健脾益气方、活血化瘀方.

  7. Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil

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    Prado José CM

    2010-04-01

    Full Text Available Abstract Background Human Papillomavirus (HPV is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation. Findings Of the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1% were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18. Conclusions Cervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts. Findings The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher

  8. [Expression of TREM-1 in patients with invasive cervical cancer and precursor lesions].

    Science.gov (United States)

    Anaya-Prado, Roberto; Norzgaray-Ibarra, Fabiola Geovanna; Bravo-Cuéllar, Alejandro; Pérez-Avila, Carlos Eduardo; Schadegg-Peña, Daniel; Anaya-Fernández, Michelle M

    2015-01-01

    Introducción: se ha demostrado que la glicoproteína TREM-1 pertenece a la superfamilia de las inmunoglobulinas que induce la secreción de varias citocinas proinflamatorias. El objetivo de este trabajo fue medir la expresión de TREM-1 en pacientes con cáncer cervical. Métodos: en este estudio transversal analítico incluimos 4 grupos de pacientes: GI: mujeres con lesión intraepitelial (LI) escamosa de bajo grado (n = 15 p/g); GII: pacientes con LI escamosa de alto grado (n = 9 p/g); GIII: pacientes con cáncer cervical invasor (n = 9 p/g), y GIV: pacientes sanas (n = 15 p/g). En todas las pacientes se midió la expresión de TREM-1 y el Índice Medio de Fluorescencia (IMF) en neutrófilos y monocitos, así como los niveles de leucocitos, neutrófilos y monocitos. Usamos t de Student para muestras independientes. Para estas mismas variables, aplicamos prueba de suma de rangos de Mann-Whitney, ANOVA y Turkey. Para las variables cualitativas se utilizó la prueba de Chi cuadrada. Resultados: los porcentaje de expresión de TREM-1 en neutrófilos y monocitos, además del IMF en neutrófilos en los 4 grupos, no fue significativamente diferente. El IMF de TREM-1 en monocitos fue significativamente diferente al comparar el grupo II y grupo III frente al grupo IV (p estudio documenta una mayor expresión de TREM-1 en monocitos de pacientes con cáncer avanzado.

  9. Serum lipid profile in patients with oral cancer and oral precancerous conditions

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    Rajul Mehta

    2014-01-01

    Full Text Available Background: The present study was undertaken to estimate and compare the levels of plasma total cholesterol (TC, low density lipoprotein (LDL, high density lipoprotein (HDL, very low density lipoprotein (VLDL and triglycerides in patients with oral precancerous lesions/conditions, oral cancer and normal subjects. Materials and Methods: The study comprised of 60 patients with oral precancerous lesions/conditions, 60 patients with oral cancer and a control group of 60 healthy individuals. The diagnosis of oral precancerous lesions/conditions and oral cancer was confirmed histopathologically. Under aseptic condition 5 ml venous blood of overnight fasting patient was withdrawn from each individual. Serum was separated by centrifugation and plasma levels of TC, LDL, HDL, VLDL and triglycerides were estimated. Descriptive statistical analysis has been carried out in the present study. Analysis of variance has been used to find the significance of study parameters between three or more groups of patients, Post-hoc test as Tukey has been used to find the pair wise significance. Significance is assessed at 5% level of significance. Results: Statistically significant decrease in levels of plasma TC, LDL, HDL, VLDL and triglycerides was observed in the precancerous and cancerous groups as compared to the control group. On comparison between precancerous and cancerous groups, significant decrease was observed in cancerous group. Conclusion: The change in lipid levels may have an early diagnostic or prognostic role in the oral premalignant lesions/conditions and oral cancer. The presence of decreased plasma lipid profile should increase the suspicion of these lesions to be investigated further.

  10. Elimination of Specular reflection and Identification of ROI: The First Step in Automated Detection of Cervical Cancer using Digital Colposcopy

    CERN Document Server

    Das, Abhishek; Bhattacharyya, Debasis

    2011-01-01

    Cervical Cancer is one of the most common forms of cancer in women worldwide. Most cases of cervical cancer can be prevented through screening programs aimed at detecting precancerous lesions. During Digital Colposcopy, Specular Reflections (SR) appear as bright spots heavily saturated with white light. These occur due to the presence of moisture on the uneven cervix surface, which act like mirrors reflecting light from the illumination source. Apart from camouflaging the actual features, the SR also affects subsequent segmentation routines and hence must be removed. Our novel technique eliminates the SR and makes the colposcopic images (cervigram) ready for segmentation algorithms. The cervix region occupies about half of the cervigram image. Other parts of the image contain irrelevant information, such as equipment, frames, text and non-cervix tissues. This irrelevant information can confuse automatic identification of the tissues within the cervix. The first step is, therefore, focusing on the cervical bor...

  11. Detection, management, and follow-up of pre-malignant cervical lesions and the role for human papillomavirus.

    NARCIS (Netherlands)

    Hamont, D. van; Bekkers, R.L.M.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2008-01-01

    Cervical cytological pathology is common. Prevention of cervical cancer by detecting the disease process at an early and pre-malignant stage is practised globally either through population-based screening programmes (PSP) or through non-organised ones. High-grade cervical intraepithelial neoplasia (

  12. Cytohistological correlation and accuracy of the pap smear test in diagnosis of cervical lesions: a hospital based cross-sectional study from Odisha, India

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    Reena Naik

    2015-09-01

    Full Text Available Background Cervical carcinoma is one of the most common causes of mortality among women.Early detection can be done by Papanicolaou (Papsmear test - a simplest, safe, cost effective and non invasive procedure. The main objective of this study was to categorize Pap smears for cytohistologicalexamination as well as clinically correlate to analyze sensitivity, specificity and positive predictive value of Pap smear. Methods In this prospective study 417 Pap smears performed, cytohistological correlation was done in 104 cases because patients had undergone both Pap smear and cervical biopsy.Detailed history was taken and clinical examination was done. Pap smear sample was collected from cervix and reporting was made according to Bethesda 2001 classification.Cytological findings were correlated with histopathology. Results Overall concordance rate was 60.7%. Concordance rate for malignancy was 100%, for inflammatory lesions 70.8% and for cervical intraepithelial neoplasia (CIN 33.3%. Common age group presented for screening was40-50 years.Commonest clinical presentationwas bleeding per vaginum. Conclusion The study provides good cyto-histopathology correlation in detecting high grade lesions and malignancy. Although Pap smear sensitivity was low but can be increased by adequate sampling and avoiding technical errors.Bethesda system is strongly recommended for adequacy of sampling to minimize inconsistency.Regular screening should be advised to the patients for the early detection of cervical carcinoma.

  13. Resolución endovascular de lesiones por arma de fuego en la región cervical

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    Sebastián Rizzone

    2016-01-01

    Full Text Available Introducción: Las heridas producidas por armas de fuego que comprometen la región cervical son materia de controversia en cuanto a cuál es el mejor método para tratarlas. La resolución endovascular de este tipo de patología ha cobrado gran relevancia en los últimos años debido al desarrollo de nuevos dispositivos y, consecuentemente, a la menor incidencia de complicaciones inherentes al procedimiento. Objetivo: Determinar el rol del tratamiento endovascular en la resolución de las lesiones cervicales por arma de fuego. Material y métodos: Se incluyeron 76 pacientes que sufrieron lesiones cervicales por arma de fuego, a los cuales se les realizó angiografía de los vasos supraaórticos para decidir la conducta a seguir. Resultados: Del total de 76 pacientes, 47 (61,8% presentaron una angiografía patológica; de estos, en 26 se decidió la resolución endovascular de la lesión, implantándose 1,1 stents por paciente. La estadía intrahospitalaria fue en promedio de 10,4 días. A los 30 días de seguimiento, 2 pacientes presentaron secuela neurológica y uno evolucionó con sepsis. En el seguimiento alejado con un promedio de 4 años se registró una muerte a los 117 días de realizado el procedimiento. Conclusiones: La intervención endovascular ha ganado mayor protagonismo con el desarrollo de nuevos dispositivos que permiten el tratamiento de patologías cada vez más complejas y reducir el tiempo de internación intrahospitalaria, como también las complicaciones relacionadas con el procedimiento.

  14. Bulky Early-Stage Cervical Cancer (2-4 cm Lesions): Upfront Radical Trachelectomy or Neoadjuvant Chemotherapy Followed by Fertility-Preserving Surgery: Which Is the Best Option?

    Science.gov (United States)

    Plante, Marie

    2015-05-01

    Radical trachelectomy is now recognized as a valid treatment option for young women with early-stage cervical cancer with lesions measuring less than 2 cm. However, for women with bulky lesions measuring greater than 2 cm, few data are available in the literature to guide management. There are currently 2 options available: either upfront radical trachelectomy or neoadjuvant chemotherapy followed by fertility-preserving surgery. Overall, both options offer very good oncologic outcome; however, the rate of fertility preservation and obstetrical outcome seem superior after neoadjuvant chemotherapy. Advantages and disadvantages of both options are discussed and a thorough literature review is provided. Issues to be further studied are also outlined.

  15. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists.

    Science.gov (United States)

    Bomfim, Rafael Aiello; Crosato, Edgard; Mazzilli, Luiz Eugênio Nigro; Frias, Antonio Carlos

    2015-01-01

    This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs) in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed) were evaluated in a Centro de Referência em Saúde do Trabalhador - CEREST (Worker's Health Reference Center). The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p smoking (p = 0.01) and age group (p = 0.04). The prevalence of NCCLs in the study population was particularly high (76.84%), and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  16. Co-infections associated with human immunodeficiency virus type 1 in pregnant women from southern Brazil: high rate of intraepithelial cervical lesions

    Directory of Open Access Journals (Sweden)

    Michele Tornatore

    2012-03-01

    Full Text Available Human immunodeficiency virus type 1 (HIV-positive pregnant women require specific prophylactic and therapeutic approaches. The efficacy of established approaches is further challenged by co-infection with other sexually transmitted diseases (STDs. The objective of this study was to determine the prevalence of co-infections in pregnant women infected with different HIV-1 subtypes and to relate these findings, together with additional demographic and clinical parameters, to maternal and infant outcomes. Blood samples from pregnant women were collected and tested for syphilis, hepatitis B virus (HBV and hepatitis C virus (HCV. Human papillomavirus (HPV diagnosis was evaluated by the presence of alterations in the cervical epithelium detected through a cytopathological exam. Medical charts provided patient data for the mothers and children. Statistical analyses were conducted with STATA 9.0. We found a prevalence of 10.8% for HCV, 2.3% for chronic HBV, 3.1% for syphilis and 40.8% for HPV. Of those co-infected with HPV, 52.9% presented high-grade intraepithelial lesions or in situ carcinoma. Prematurity, birth weight, Apgar 1' and 5' and Capurro scores were similar between co-infected and non-co-infected women. The presence of other STDs did not impact maternal and concept outcomes. More than half of the patients presenting cervical cytology abnormalities suggestive of HPV had high-grade squamous intraepithelial lesions or cervical cancer, evidencing an alarming rate of these lesions.

  17. Risk factors for cervical carotid and intracranial cerebrovascular lesions in patients undergoing coronary artery bypass grafting. Preoperative evaluation using magnetic resonance imaging and angiography

    Energy Technology Data Exchange (ETDEWEB)

    Makino, Masahiro [Kyoto Prefectural Univ. of Medicine (Japan). Research Inst. for Neurological Diseases and Geriatrics

    2001-12-01

    Recently neurologic complications after coronary artery bypass grafting (CABG) have received increasing attention. There is no detailed report about the risk factors for these complications, although stenosis in the cervical and intracranial arteries, especially in Japanese patients, latent ischemic brain lesions and preoperative neurological conditions are related to these events. In this prospective study, we evaluated occlusive lesions in the cervical carotid and intracranial arteries, silent brain infarction and cerebral deep white matter lesion with MRA and MRI in patients scheduled to undergo CABG to determine the prevalence of occlusive diseases in cervical carotid and intracranial arteries, latent ischemic change in the brain in this population and to identify preoperative risk factors for these patients. The subjects were 144 consecutive patients (103 men and 41 women, mean age 65.9{+-}9.2 years old) who were scheduled for CABG under elective conditions and who were examined by the same MRI apparatus using the same protocol between November 1998 and March 2001. After routine neurological examination and Mini-Mental State Examination (MMSE) were completed, MRI and MRA were obtained, then the prevalence of abnormalities on MRI and MRA studies and risk factors were evaluated. Cervical carotid artery stenosis with {>=}50% luminal narrowing was detected in 29.2% of the subjects, and that with {>=}75% luminal narrowing was detected in 16.0% of the subjects. Intracranial arterial stenosis showing {>=}50% luminal narrowing was detected in 38.2% of subjects, and that showing {>=}75% luminal narrowing was detected in 19.4% of subjects. Brain infarction was observed in 74.3% of subjects, cerebral deep white matter lesion showing grade 2 or higher on Fazekas classification was observed in 17.4% of the subjects. The characteristics, including possible risk factors of subjects with and without these abnormal findings, were compared. Patients with cervical carotid

  18. Risk factors for cervical carotid and intracranial cerebrovascular lesions in patients undergoing coronary artery bypass grafting. Preoperative evaluation using magnetic resonance imaging and angiography

    International Nuclear Information System (INIS)

    Recently neurologic complications after coronary artery bypass grafting (CABG) have received increasing attention. There is no detailed report about the risk factors for these complications, although stenosis in the cervical and intracranial arteries, especially in Japanese patients, latent ischemic brain lesions and preoperative neurological conditions are related to these events. In this prospective study, we evaluated occlusive lesions in the cervical carotid and intracranial arteries, silent brain infarction and cerebral deep white matter lesion with MRA and MRI in patients scheduled to undergo CABG to determine the prevalence of occlusive diseases in cervical carotid and intracranial arteries, latent ischemic change in the brain in this population and to identify preoperative risk factors for these patients. The subjects were 144 consecutive patients (103 men and 41 women, mean age 65.9±9.2 years old) who were scheduled for CABG under elective conditions and who were examined by the same MRI apparatus using the same protocol between November 1998 and March 2001. After routine neurological examination and Mini-Mental State Examination (MMSE) were completed, MRI and MRA were obtained, then the prevalence of abnormalities on MRI and MRA studies and risk factors were evaluated. Cervical carotid artery stenosis with ≥50% luminal narrowing was detected in 29.2% of the subjects, and that with ≥75% luminal narrowing was detected in 16.0% of the subjects. Intracranial arterial stenosis showing ≥50% luminal narrowing was detected in 38.2% of subjects, and that showing ≥75% luminal narrowing was detected in 19.4% of subjects. Brain infarction was observed in 74.3% of subjects, cerebral deep white matter lesion showing grade 2 or higher on Fazekas classification was observed in 17.4% of the subjects. The characteristics, including possible risk factors of subjects with and without these abnormal findings, were compared. Patients with cervical carotid lesions were

  19. Anti-Nogo-A antibody treatment does not prevent cell body shrinkage in the motor cortex in adult monkeys subjected to unilateral cervical cord lesion

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    Mir Anis

    2008-01-01

    Full Text Available Abstract Background After unilateral cervical cord lesion at the C7/C8 border interrupting the dorsolateral funiculus in adult monkeys, neutralization of Nogo-A using a specific monoclonal antibody promoted sprouting of corticospinal (CS axons rostral and caudal to the lesion and, in parallel, improved functional recovery. In monkeys lesioned but not treated with the anti-Nogo-A antibody, the CS neurons in the contralesional primary motor cortex (M1 survived to the axotomy, but their soma shrank. Because the anti-Nogo-A treatment induces regeneration and/or sprouting of CS axons, it may improve access to neurotrophic factors. The question therefore arises as to whether anti-Nogo-A treatment prevents the soma shrinkage observed in the contralesional M1? Results Using the marker SMI-32, a quantitative and qualitative anatomical assessment of the pyramidal neurons in the layer V (thus including the CS cells in M1 was performed and compared across three groups of animals: intact monkeys (n = 5; monkeys subjected to the cervical cord lesion and treated with a control antibody (n = 4; monkeys with the cervical lesion and treated with anti-Nogo-A antibody (n = 5. SMI-32 positive neurons on the side contralateral to the lesion were generally less well stained than those on the ipsilesional hemisphere, suggesting that they expressed less neurofilaments. Nevertheless, in all three groups of monkeys, the amount of SMI-32 positive neurons in both hemispheres was generally comparable, confirming the notion that most axotomized CS neurons survived. However, shrinkage of CS cell body area was observed in the contralesional hemisphere in the two groups of lesioned monkeys. The cell surface shrinkage was found to be of the same magnitude in the monkeys treated with the anti-Nogo-A antibody as in the control antibody treated monkeys. Conclusion The anti-Nogo-A antibody treatment did not preserve the axotomized CS cells from soma shrinkage, indicating that the

  20. P16INK4a: a potential diagnostic adjunct for prediction of high-grade cervical lesions in liquid-based cytology: with HPV testing and histological correlation.

    Science.gov (United States)

    Wong, Y P; Abdul Raub, S H; Mohd Dali, A Z H; Kassim, F; Visvalingam, V; Zakaria, Z; Kamaluddin, M A; Noor Akmal, S

    2016-08-01

    Human papillomavirus (HPV) is a necessary cause of cervical cancer and its precursors. Increased expression of high-risk hrHPV viral oncogenes in abnormal cells might increase the expression of p16INK4a. We aimed to determine the role of p16INK4a in detecting hrHPV-transformed epithelial cells in liquid-based cervical cytology, and compared the results with hrHPV DNA testing by realtime polymerase chain reaction (RT-PCR). Fifty-seven cytological samples were tested for p16INK4a immunomarker and hrHPV DNA. Test performance of both tests was determined by comparing sensitivity, specificity and predictive values using available histological follow-up data as gold standard. Of 57 samples, 36 (63.2%) showed immunoreactivity for p16INK4a and 43 (75.4%) were hrHPV-infected. A fairly low concordance rate (k = 0.504) between p16INK4a immunolabelling and hrHPV DNA status was noted. For prediction of cervical intraepithelial neoplasia (CIN) II and worse lesions, p16INK4a had a sensitivity and specificity of 93.5% and 60%; whereas hrHPV DNA testing had a sensitivity and specificity of 100% and 20%. Dual testing by combining p16INK4a and hrHPV showed sensitivity and specificity of 100% and 33.3%. In conclusion, p16INK4a is useful in predicting severity of the cytological abnormalities. Although p16INK4a is more specific but less sensitive than hrHPV in detecting high-grade cervical lesions, a combination of both tests failed to demonstrate significant improvement in diagnostic sensitivity, specificity and predictive value. Larger-scale prospective studies are required to assess further whether this biomarker should be routinely used as primary screening tool independently or in combination with hrHPV testing to improve diagnostic accuracy in cervical cytology. PMID:27568665

  1. RARβ在宫颈癌及癌前病变中的表达调控和意义%The association of altered RARβ gene expression with the cervical lesion pathogenesis

    Institute of Scientific and Technical Information of China (English)

    Nafeisha Kadeer; Hui He; Abida Abudoukadeer; Anaguli Ababaikeli; Abulizi Abudula

    2011-01-01

    Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β (RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was to investigate the association of cervical cancer development with the RARβ gene expression at the mRNA and protein level to assess the impact of RARβ as a marker for early detection of the cancer.Methods:We collected 126 cases of formalin fixed and paraffin embedded cervical tissue specimens as well as 37 cases of fresh tissue samples from women with cervicitis,cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC).The RARβ mRNA and protein expression was detected by quantitative RT-PCR and immunohistochemistry,respectively.Results:(1) The mRNA expression of RARβ in CIN and cervical cancer was markedly decreased compared to cervicitis with a statistically very significant difference,but no difference was found between CIN and cervical cancer.(2) RARβ protein was normally expressed in the epithelial cells of cervicitis and partially lost in a few cases,but with the development of cervical lesion pathogenesis and cancer,a significant loss of protein expression was detected in CIN (38%) and CSCC (57%) compared to cervicitis (P < 0.01).Conclusion:The downregulation of RARβ transcription or loss of protein expression is an important indicator of cervical cancer and its precursur lesions.The detection of RARβ expression coupled with aberrant methylation of the gene may become a biomarker for the early prognosis or diagnosis of the cancer.

  2. 内镜下卢戈液染色联合窄带成像对食管癌及癌前病变的诊断分析%Diagnostic analysis of endoscopic Lugol solution staining combined with narrow band imaging for ;esophageal cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    杨彬; 陈滟

    2015-01-01

    目的:探讨内镜下卢戈液染色联合窄带成像(NBI)对食管癌及癌前病变的诊断价值。方法选择2013年12月至2014年12月在四川省遂宁市中心医院行胃镜检查,存在可疑食管病灶而进行卢戈液染色和病理学检查的187例患者作为对照组,选择2015年1月至2015年6月行胃镜检查,存在可疑食管病灶而进行卢戈液染色联合 NBI 检查和病理学检查的124例患者作为观察组。对两组检查的敏感性、特异性和准确性进行比较。结果观察组患者内镜下检测阳性率为75.0%,高于对照组的55.6%,差异有统计学意义(χ2=12.07,P <0.01)。观察组患者的食管癌和高级别内瘤变检出率分别为29.0%和17.7%,均高于对照组的18.2%和6.4%,且差异有统计学意义(χ2=4.15,P =0.04;χ2=8.54,P <0.01)。观察组患者内镜下检测结果与病理学检查结果的 Kappa 值=0.55,对照组患者的Kappa值=0.36。观察组检测方法的敏感性和准确性分别为89.9%和82.3%,高于对照组的71.8%和68.4%,且差异均有统计学意义(χ2=9.79,P <0.01;χ2=7.38,P <0.01)。结论卢戈液染色联合 NBI对食管癌和食管癌前病变检测的敏感性和检出率优于单纯性卢戈液染色方法,值得在早期食管癌及癌前病变诊断中进行推广。%Objective To investigate the diagnostic value of Lugol solution staining combined with nar-row band imaging (NBI)for esophageal cancer and precancerous lesions.Methods From December 201 3 to December 2014,1 87 patients who were with suspicious esophageal lesions and stained with Lugol solution under endoscopy were selected as control group,while from January 2015 to June 2015,124 patients who were with sus-picious esophageal lesions and examined with Lugol solution staining and NBI under endoscopy were selected as observation group.The sensitivity

  3. AN UPWARD TREND IN DNA P16INK4A METHYLATION PATTERN AND HIGH RISK HPV INFECTION ACCORDING TO THE SEVERITY OF THE CERVICAL LESION

    Directory of Open Access Journals (Sweden)

    Fernanda Nahoum Carestiato

    2013-09-01

    Full Text Available SUMMARY High-risk human papillomavirus (hr-HPV infection is necessary but not sufficient for cervical cancer development. Recently, P16INK4A gene silencing through hypermethylation has been proposed as an important cofactor in cervical carcinogenesis due to its tumor suppressor function. We aimed to investigate P16INK4A methylation status in normal and neoplastic epithelia and evaluate an association with HPV infection and genotype. This cross-sectional study was performed with 141 cervical samples from patients attending Hospital Moncorvo Filho, Rio de Janeiro. HPV detection and genotyping were performed through PCR and P16INK4A methylation by nested-methylation specific PCR (MSP. HPV frequency was 62.4% (88/141. The most common HPV were HPV16 (37%, HPV18 (16.3% and HPV33/45(15.2%. An upward trend was observed concerning P16INK4A methylation and lesion degree: normal epithelia (10.7%, low grade lesions (22.9%, high grade (57.1% and carcinoma (93.1% (p < 0.0001. A multivariate analysis was performed to evaluate an association between methylation, age, tobacco exposure, HPV infection and genotyping. A correlation was found concerning methylation with HPV infection (p < 0.0001, hr-HPV (p = 0.01, HSIL (p < 0.0007 and malignant lesions (p < 0.0001. Since viral infection and epigenetic alterations are related to cervical carcinoma, we suggest that P16INK4A methylation profile maybe thoroughly investigated as a biomarker to identify patients at risk of cancer.

  4. 宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用分析%Application Analysis the Cervical Scraping Cytology in Screening of Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张鸥

    2016-01-01

    目的:对宫颈刮片脱落细胞学检查在筛选宫颈癌中的应用进行观察分析。方法选取我院2014年1月~2015年12月收治的214例进行宫颈癌筛查患者作为研究对象,进行宫颈刮片脱落细胞学检查,将宫颈刮片脱落细胞学检查结果同宫颈活组织病理检查相比较,观察分析宫颈刮片脱落细胞学检查在诊断宫颈癌、癌前病变的敏感度、特异度、阴性预测值和阳性预测值等。结果宫颈刮片脱落细胞学检查诊断宫颈癌、癌前病变敏感度为90.43%,特异度为89.17%,阳性预测值为86.73%,阴性预测值为92.24%,诊断准确率为89.72%。结论宫颈刮片脱落细胞学检查在筛查宫颈癌方面具有较高的敏感度。%Objective To observe and analyze the application of cervical scraping cytology in screening of cervical cancer.Methods Selected 214 cases of patients with cervical cancer screening from January 2014 to December 2015 in our hospital, for example, cervical scraping cytology, cervical scraping cytology results with cervical biopsy compared toobserve and analyze the cervical scraping off cytology in the diagnosis of cervical cancer and precancerous lesions of sensitivity, speciifcity, negative predictive and positive predictive value.Results The sensitivity of cervical cancer and precancerous lesion was 90.43%, the speciifcity was 89.17%, the positive predictive value was 86.73%, the negative predictive value was 92.24%, the diagnostic accuracy rate was 89.72%.Conclusion Cervical scraping cytology test in the screening of cervical cancer has a high sensitivity.

  5. Stress Analysis of Occlusal Forces in Canine Teeth and Their Role in the Development of Non-Carious Cervical Lesions: Abfraction

    Directory of Open Access Journals (Sweden)

    Shihab A. Romeed

    2012-01-01

    Full Text Available Non-carious cervical tooth lesions for many decades were attributed to the effects of abrasion and erosion mainly through toothbrush trauma, abrasive toothpaste, and erosive acids. However, though the above may be involved, more recently a biomechanical theory for the formation of these lesions has arisen, and the term abfraction was coined. The aim of this study was to investigate the biomechanics of abfraction lesions in upper canine teeth under axial and lateral loading conditions using a three-dimensional finite element analysis. An extracted human upper canine tooth was scanned by μCT machine (Skyscan, Belgium. These μCT scans were segmented, reconstructed, and meshed using ScanIP (Simpleware, Exeter, UK to create a three-dimensional finite element model. A 100 N load was applied axially at the incisal edge and laterally at 45° midpalatally to the long axis of the canine tooth. Separately, 200 N axial and non-axial loads were applied simultaneously to the tooth. It was found that stresses were concentrated at the CEJ in all scenarios. Lateral loading produced maximum stresses greater than axial loading, and pulp tissues, however, experienced minimum levels of stresses. This study has contributed towards the understanding of the aetiology of non-carious cervical lesions which is a key in their clinical management.

  6. SPECTRUM OF CERVICAL LESIONS IN CIMS, BILASPUR: A 5 YEAR RETROSPECTIVE STUDY OF 215 CASES IN A TERTIARY HOSPITAL OF CENTRAL INDIA

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    Sadhna

    2015-10-01

    Full Text Available BACKGROUND AND OBJEC TIVE: Cancer of the cervix is a leading cause of morbidity and mortality among women W orld W ide. Therefore to curb the disease there is need of awareness of this disease. This study is aimed to categorize the premalignant and malignant lesions at the earliest, to reduce the mortality and morbidity. It also aimed to know the incidence of malignancies at our set up and to calculate distribution of patient in relation to parity and symptoms. DESIGN AND SETTING: Study included 215 patients with complain of white dis charge per vagina, per vaginal bleeding and backache attending the gynecology OPD over a period of 05 years. PATIENT AND METHODS: Patients were subjected to cervical biopsy and biopsies were sent to pathology department. Detailed clinical history, age, age at marriage, parity and socio - economic status was obtained. RESULT: Result were classified histopathological l y as inflammatory lesions 70 cases, polypoidal lesion were 30, LSIL accounted for 32 cases and HSIL were 20 cases and 30 were malignant lesions. U nder malignant category moderately differentiated squamous cell carcinoma was the commonest. Maximum numbers of patients were subjected to cervical biopsies in 3 rd decade of life. Malignancies were common in 3 rd & 4 th decade of life. Most of the cases were of parity 4. CONCLUSION : Tissue biopsy is a valuable diagnostic procedure on which surgeons and radiologists still rely.

  7. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists

    Directory of Open Access Journals (Sweden)

    Rafael Aiello BOMFIM

    2015-01-01

    Full Text Available This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed were evaluated in a Centro de Referência em Saúde do Trabalhador – CEREST (Worker’s Health Reference Center. The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p < 0.05. NCCLs were significantly associated with age groups (18-34, 35-44, 45-68 years. The unconditional logistic regression showed that the presence of NCCLs was better explained by age group (OR = 4.04; CI 95% 1.77-9.22 and occupational exposure to acid mists and chemical products (OR = 3.84; CI 95% 1.10-13.49, whereas the linear multiple regression revealed that NCCLs were better explained by years of smoking (p = 0.01 and age group (p = 0.04. The prevalence of NCCLs in the study population was particularly high (76.84%, and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  8. 醋酸联合靛胭脂染色诊断早期胃癌及癌前病变%The clinical application of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    胡立华; 陈鹏; 张继晔; 王燕颖; 王文多; 张沛怡

    2014-01-01

    目的 探讨内镜下醋酸联合靛胭脂染色法对早期胃癌及癌前病变的临床诊断价值.方法 2011年1-12月在我院就诊患者行常规胃镜检查,发现可疑病灶者300例,随机分为两组,染色组(150例)行内镜下醋酸联合靛胭脂染色后活检,对照组(150例)行内镜下靛胭脂染色后活检.结果 染色组150例中共检出早期胃癌16例(10.7%)、重度不典型增生15例(10.0%)、中度不典型增生、肠上皮化生79例(52.7%),其余40例为胃炎.对照组150例中检出早期胃癌5例(3.3%)、重度不典型增生10例(6.7%)、中度不典型增生、肠上皮化生42例(28.0%),其余93例为胃炎.染色组早期胃癌及癌前病变检出率明显高于对照组,差异有统计学意义(P <0.001).醋酸联合靛胭脂染色后早期胃癌和重度不典型增生黏膜主要表现为黏膜退色,中度不典型增生和肠上皮化生黏膜主要表现为黏膜着色不良,慢性胃炎和正常黏膜主要表现为着色均匀.结论 内镜下醋酸联合靛胭脂染色可显著提高早期胃癌和癌前病变的检出率,具有较高的临床应用价值.%Objective To investigate the significance of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion.Methods After conventional electronic endoscopic diagnosis,From 2011 fanuay to 300 patients of suspicious lesions were randomly divided into endoscopic dye group and control group,each was 150 cases.Patients of endoscopic dye group were directly performed biopsy after acetic acid and indigo carmine dye,while in control group were performed biopsy after only indigo carmine dye.Results In the endoscopic dye group,16 early neoplasia (10.7%),15 severe atypical hyperplasia (10.0%),79 moderate atypical hyperplasia or Intestinal metaplasia (52.7%) and 40 chronic gastritis were found.In the control group,5 early neoplasia (3.3%),10 severe atypical hyperplasia (6.7%),42

  9. Comparison of conventional Papanicolaou smear and SurePath® liquid-based cytology in the Copenhagen population screening programme for cervical cancer

    DEFF Research Database (Denmark)

    Kirschner, Benny; Simonsen, Kåre; Junge, Jette

    2006-01-01

    OBJECTIVE: To compare diagnostic performance of conventional Papanicolaou smear with SurePath liquid-based cytology in a population screening programme. METHODS: A retrospective comparison was performed on data from two 18-month periods of the screening programme for cervical cancer...... in the municipality of Copenhagen with conventional Papanicolaou technique (n = 82,116) and liquid-based cytology (n = 84,414). RESULTS: After the conversion to liquid-based cytology the percentage of unsatisfactory samples decreased from 2.3% to 0.3% (P cervical samples lacking......-based technique. CONCLUSIONS: This study showed the number of unsatisfactory samples to be significantly reduced with the liquid-based technique. The data suggest that there is an increased detection rate of cervical precancerous lesions with liquid-based cytology, but the number of false positive tests is still...

  10. HPV16-E2 induces prophase arrest and activates the cellular DNA damage response in vitro and in precursor lesions of cervical carcinoma.

    Science.gov (United States)

    Xue, Yuezhen; Toh, Shen Yon; He, Pingping; Lim, Thimothy; Lim, Diana; Pang, Chai Ling; Abastado, Jean-Pierre; Thierry, Françoise

    2015-10-27

    Cervical intraepithelial neoplasia (CIN) is caused by human papillomavirus (HPV) infection and is the precursor to cervical carcinoma. The completion of the HPV productive life cycle depends on the expression of viral proteins which further determines the severity of the cervical neoplasia. Initiation of the viral productive replication requires expression of the E2 viral protein that cooperates with the E1 viral DNA helicase. A decrease in the viral DNA replication ability and increase in the severity of cervical neoplasia is accompanied by simultaneous elevated expression of E6 and E7 oncoproteins. Here we reveal a novel and important role for the HPV16-E2 protein in controlling host cell cycle during malignant transformation. We showed that cells expressing HPV16-E2 in vitro are arrested in prophase alongside activation of a sustained DDR signal. We uncovered evidence that HPV16-E2 protein is present in vivo in cells that express both mitotic and DDR signals specifically in CIN3 lesions, immediate precursors of cancer, suggesting that E2 may be one of the drivers of genomic instability and carcinogenesis in vivo. PMID:26474276

  11. New paradigms in cervical cancer prevention: opportunities and risks

    Directory of Open Access Journals (Sweden)

    Giorgi Rossi Paolo

    2008-12-01

    Full Text Available Testing for the DNA of high-risk types of papilloma virus (HPV is more sensitive than cytology in detecting pre-cancerous lesions. One of the main advantages will be the possibility of applying prolonged screening intervals. However adequate screening protocols (age of start and stop, screening intervals, management of HPV positive women need to be applied in order to avoid over-referral to colposcopy and over-treatment and to maintain sustainable costs. Further follow-up of running trials and research on molecular markers will better define these parameters. The new situation will require organised screening programmes with rigorous protocols and monitoring. This will be even more needed when women vaccinated for HPV 16 and 18 will be screened. Research on how to best screen vaccinated women is a priority. This paper proposes an overview of the plausible impact of new technologies in cervical cancer screening in the near future and in the vaccinated cohorts.

  12. 宫颈病变筛查策略的效果分析%The Evaluation of the Effect of Cervical Lesions Screening Strategies

    Institute of Scientific and Technical Information of China (English)

    龙腾飞

    2011-01-01

    Cervical cancer is one of the most common female malignant cancers , with an increasing incidence and earlier onset ages. In developed countries, the systematic screening programmes have reduced the morbidity and mortality resulting from cervical cancer. The screening program is the main means to prevent and control cervical cancer. The main screening tools are visual inspection, cytology, human papillomavirus testing and colposcopy. The scholars all over the world made great efforts to seek appropriate cervical cancer screening solutions in either single or comhines use of various methods. Here is to review on the effert of cervical lesions screening strategies in recent years inside and outside China.%宫颈癌是妇科常见的恶性肿瘤之一,其发病率逐年升高,且发病年龄有年轻化的趋势.对宫颈癌的筛查是预防和控制宫颈癌的主要手段,也是极大限度防治宫颈癌的最好途径.宫颈癌筛查方法包括肉眼观察、宫颈细胞学、人类乳头瘤病毒检测及阴道镜检查等.世界各地学者做了许多努力来寻求适宜的宫颈癌筛查方案,包括各种方法的单独及联合使用.现就近年来国内外宫颈病变筛查策略的效果予以综述.

  13. P73、ER、PR在食管癌及癌前病变中的表达及临床意义%Expression of p73, ER and PR in esophageal carcinoma and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    樊圣; 艾杰; 王军

    2006-01-01

    Objective: To test the expression of mutant p73, p53, ER and PR proteins in the esophageal normal mucosa, hyperplasia, dysplasia and squamous cell carcinoma, and research the clinically pathological significance and the correlation, for the early diagnosis, prognostic measure and therapy in clinic. Methods: With Immunohistochemistry, it was examined to show these tumor markers' expression in different epithelial lesions of 40 esophageal squamous cell carcinomas, 14 dysplasias, 14 hyperplasias and 14 normal mucosas. Results: The expression of p73 was 55%, 21%, 0% and 0% in the esophageal carcinoma, dysplasia, hyperplasia and normal mucosa, respectively. The significant difference in expression of p73 (P<0.001)was observed between the esophageal normal mucosia, hyperplasia, dysplasia and esophageal squamous cell carcinoma with Fisher's exact test. Difference in expression of p73 (P<0.05) was observed between the esophageal squamous cell carcinoma and dysplasia with χ2 test. The expression of p73 showed non-correlation with the patient's age, sex, tumor's grade, lymph-node metastasis and invasive depth (P>0.05); Similarly, the expression of mutant p53 was 67.5%, 35.7%, 7% and 0%, respectively; In like manner, the expression of ER was 55%, 21.4%, 14.2% and 0%, respectively; The expression of PR was 57.5%,14.28%, 0%and 0%, respectively. The significant difference in expression of PR (P<0.001) was observed with Fisher's exact test. Difference in expression of PR (P<0.05) was observed between the esophageal squamous cell carcinoma and dysplasia with χ2 test. The expression of PR (P<0.05) was correlated with lymph-node metastasis, and showed non-correlation with the patient's age, sex, tumor's grade, and invasive depth (P>0.05). Moreover, over-expression of mutant p53 and p73 showed significant correlation with ER and PR protein's positive expression (P<0.05). Conclusion: P73 protein may become a new tumor's marker to diagnose esophageal squamous cell

  14. 电子染色内镜对早期胃癌及癌前病变边界的评估价值%The Assessment Value of Electronic Staining Endoscopy for Early Gastric Cancer and Precancerous Lesions Border

    Institute of Scientific and Technical Information of China (English)

    夏阳

    2014-01-01

    Objective Discuss the assessment value of electronic staining endoscopy for early gastric cancer and precan -cerous lesions border .Methods 212 patients with gastroscope inspection were selected ,patients were divided into group A ( e-lectron staining endoscopy group ) and group B (normal endoscopy group ),each 106 cases.compared two groups of gastroscope inspection result .Results Electron staining endoscopy and common endoscopic detection of atrophic gastritis and intestinal meta -plasia was no statistically difference in diagnosis accuracy rate ,P>0.05.Electron staining endoscopy diagnosis of atypical hyper-plasia and early gastric cancer was significantly higher than that of ordinary endoscopy ,P<0.05.Conclusion Using electronic dyeing endoscopic biopsy under the guidance can enhance the detection rate of dysplasia and early gastric cancer .%目的:探讨电子染色内镜对早期胃癌及癌前病变边界的评估价值。方法选择在我院进行胃镜检查的患者共212例,根据检查方法不同分为A组(电子染色内镜组)及B组(普通内镜组),每组106例,对比2组胃镜检测效果。结果电子染色内镜与普通内镜检测萎缩性胃炎及肠上皮化生的诊断准确率差异无统计学意义,P>0.05。电子染色内镜检测不典型增生和早期胃癌的准确率显著高于普通内镜,P<0.05。结论在电子染色内镜指导下进行活检,可以提高不典型增生和早期胃癌的检出率。

  15. [Unstable traumatic lesions of the lower cervical spine without neurologic deficiency. Our experience at the Fondation Jeanne Ebori in Libreville (Gabon)].

    Science.gov (United States)

    Loembe, P M; Chouteau, Y; Bouger, D

    1991-04-01

    23 cases of unstable traumatic lesions of the lower cervical spine, without neurologic complication, were treated over a period of eight years. These cases consisted of eight dislocations, five subluxations, nine fracture-dislocations and one "tear drop" or fracture of the vertebral body. Six cases were treated conservatively and fifteen operatively. Two patients died in skeletal traction while awaiting surgical intervention. The surgical approaches were anterior (6 cases), posterior (8 cases) and combined (1 case). There was one case of transitory neurologic deficit immediately after surgery. One case required re-intervention. Overall they obtained satisfactory osteoligamentary consolidation. PMID:2055985

  16. Papanicolau smear chances to be diagnostic for cervical squamous intraepithelial lesions (SIL) with or without detectable HPV DNA at in situ hybridization analysis.

    Science.gov (United States)

    Sopracordevole, F; Cadorin, L; Muffato, G; De Benetti, L; Parin, A

    1993-01-01

    The Authors have correlated 39 cervical diagnostic biopsies for squamous intraepithelial lesions (SILs) with correspective Papanicolau smears (PS), with relation to the presence or the absence of HPV of oncogenic type (HPV-one) detected by in situ hybridization (ISH). Agreement between cytological and histological diagnosis was present in 14 of 16 cases with detectable HPV-one and only in 12 of 23 cases without detectable HPV-one at ISH. The importance of the HPV type in the SILs with relation to the diagnostic accuracy of Papanicolaou smears has been discussed.

  17. Human papillomavirus (HPV detected in restored plasma DNA from women diagnosed with pre-invasive lesions and invasive cervical cancer

    Directory of Open Access Journals (Sweden)

    Edward Fabián Carrillo

    2010-06-01

    Full Text Available Objective: To improve the sensitivity of Human Papillomavirus (HPV detection in plasma from high-grade cervical neoplasia patients (CIN III and cervical cancer (CC evaluating any likely correlation with disease stage.Method: We subjected plasma DNA isolates from 112 patients (CIN and ICC to a pre-PCR restoration treatment to improve detection sensitivity. HPV-specific sequences were detected by conventional PCR both in cervical scrapes and plasma DNA obtained from each patient. For every single DNA sample, both non-restored and restored isolates were PCR analyzed.Results: We detected HPV in plasma DNA isolates with significantly higher efficiency on restored plasma-DNA as compared to each non-restored equivalent, still maintaining close correlation with the clinical stage of the cases. By analyzing plasma-DNA isolates we could classify as HPV positive >50.0% of the cases that were previously known to be positive from the cervical scrape based assay. Interestingly, 100% of the cases in which subtype HPV18 was detected in cervical scrapes were also positive in plasma DNA.Conclusions: Restoration of plasma DNA from cervical cancer patients allows a more sensitive PCR-based HPV detection, maintaining the correlation to disease stage traditionally observed.

  18. 幽门螺杆菌感染在喉癌及癌前病变中与COX-2表达的相关研究%The relevant research of helicobacter pylori infection in laryngeal cancer and precancerous lesions and cox-2 expression

    Institute of Scientific and Technical Information of China (English)

    杨艳青; 张丽萍; 薛振伟; 王立坤; 刁玉华

    2014-01-01

    目的:检测喉癌及癌前病变组织中的HP(幽门螺杆菌)感染及环氧化酶-2(coX-2)表达,为寻找更有效的喉癌预防措施提供理论依据。方法免疫组化法和硼酸亚甲蓝法分别检测50例喉单纯增生、喉癌前病变、喉癌中HP感染情况和coX-2表达。结果喉单纯性增生、喉癌前病变、喉癌HP感染阳性率分别为:32%、66%、24%,P<0.05;coX-2表达阳性率分别为:18%、46%、80%,P<0.05;HP阳性组coX-2表达均高于HP阴性组,其中,HP阳性组喉癌前病变coX-2表达高于HP阴性组,P<0.05。结论1、HP感染可能是喉癌发生的一个早期事件;将为喉癌前期病变的干预性治疗提供一个临床的参考指标。2、coX-2的表达与喉癌的发生、发展可能有关。3、HP感染后诱导喉癌前病变中coX-2的表达,可能是HP致癌机制之一。%Objective By detecting the relationship between helicobacter pylori(HP) infection and the expression of cox-2 in laryngeal cancer and precancerous tissue,as to provide theoretical basis for looking for effective laryngeal cancer prevention measures. Methods the specimens from 50 cases of laryngeal simple hyperplastic , lesion before larynx cancer and laryngeal cancer ,were detected the diversity of positive expression rates of cox-2 by immunohistochemical method and detection of HP infection by improved borate methylene blue method. Result in throat simple hyperplastic,lesion before larynx cancer and laryngeal cancer:the positive rate of HP infection is respectively:32%,66%, 24%,P<0.05;the positive rate of cox-2 is respectively:18%,46%80%,P<0.05;Positive expression of cox-2 in HP positive group were higher than in HP negative group,among them,positive expression of cox-2 in HP positive group were higher than in HP negative group in the lesion before larynx cancer,P<0.05.Conclusion 1, HP infection may be an early event of laryngeal cancer and provide a clinical reference index with the

  19. Age trends in the prevalence of cervical squamous intraepithelial lesions among HIV-positive women in Cameroon: a cross-sectional study

    LENUS (Irish Health Repository)

    Atashili, Julius

    2012-10-29

    AbstractBackgroundCervical squamous intra-epithelial lesions (SIL) are more frequent in HIV-positive women overall. However the appropriate age at which to begin and end cervical cancer screening for early detection of lesions in HIV-positive women is not clear. We assessed the age-specific prevalence of any SIL and SIL requiring colposcopy in HIV-positive women in Cameroon.MethodsWe enrolled, interviewed and conducted conventional cervical cytology in 282 women, aged 19--68 years, initiating antiretroviral therapy in three clinics in Cameroon. In bivariable analyses, the crude relationship between age and the presence of lesions was assessed using locally weighted regression (LOWESS) methods. In multivariate analyses, generalized linear models with prevalence as the outcome, an identity link and a binomial distribution, were used to estimate prevalence differences. Bias analyses were conducted to assess the potential effect of inaccuracies in cytology.ResultsSIL were detected in 43.5% of the 276 women with satisfactory samples, 17.8% of whom had ASC-H\\/HSIL. On average, women aged 26 to 59 tended to have a slightly higher prevalence of any SIL than other women (Prevalence difference PD: 6.5%; 95%CI: -11.4, 24.4%). This PD was a function of CD4 count (heterogeneity test p-value =0.09): amongst patients with CD4 counts less than 200cells\\/uL, the prevalence was higher in patients aged 26--59, while there was essentially no difference amongst women with CD4 counts greater than 200 cells\\/uL. ASC-H\\/HSIL were present in women as young as 19 and as old as 62. Overall the prevalence of ASC-H\\/HSIL increased by 0.7% (95%CI: -3.8%, 5.1%) per decade increase in age.ConclusionBoth severe and less severe lesions were prevalent at all ages suggesting little utility of age-targeted screening among HIV-positive women. Nevertheless, the long-term evolution of these lesions needs to be assessed in prospective studies.

  20. Clinical interest of postural and vestibulo-ocular reflex changes induced by cervical muscles and skull vibration in compensated unilateral vestibular lesion patients.

    Science.gov (United States)

    Dumas, Georges; Lion, Alexis; Gauchard, Gérome C; Herpin, Guillaume; Magnusson, Måns; Perrin, Philippe P

    2013-01-01

    Skull vibration induces nystagmus in unilateral vestibular lesion (UVL) patients. Vibration of skull, posterior cervical muscles or inferior limb muscles alters posture in recent UVL patients. This study aimed to investigate the postural effect of vibration in chronic compensated UVL patients. Vibration was applied successively to vertex, each mastoid, each side of posterior cervical muscles and of triceps surae in 12 UVL patients and 9 healthy subjects. Eye movements were recorded with videonystagmography. Postural control was evaluated in eyes open (EO) and eyes closed (EC) conditions. Sway area, sway path, anteroposterior and medio-lateral sways were recorded.A vibration induced nystagmus (VIN) beating toward the healthy side was obtained for each UVL patient during mastoid vibration. In EO, only sway path was higher in UVL group during vibration of mastoids and posterior cervical muscles.The EO postural impairments of UVL patients could be related to the eye movements or VIN, leading to visual perturbations, or to a proprioceptive error signal, providing an erroneous representation of head position. The vibration-induced sway was too small to be clinically useful. Vestibulo-ocular reflex observed with videonystagmography during mastoid vibration seems more relevant to reveal chronic UVL than vestibulo-spinal reflex observed with posturography.

  1. Diagnosing Cervical Dysplasia Using Visual Inspection of the Cervix with Acetic Acid in a Woman in Rural Haiti

    Directory of Open Access Journals (Sweden)

    Elizabeth Roger

    2014-11-01

    Full Text Available Cervical cancer remains a significant cause of morbidity and mortality for women in developing countries, despite the fact that inexpensive, simple and effective screening methods are available. Visual inspection of the cervix with acetic acid (VIA can be used as part of a “screen and treat” program to identify precancerous lesions for cryotherapy treatment. This case report details how the VIA screening test was incorporated into the care of a patient presenting to a maternal health clinic in Thomonde, Haiti which was staffed by doctors and medical students from Emory University School of Medicine in collaboration with Haiti Medishare. As demonstrated here, the VIA test requires minimal materials, can be efficiently incorporated into a physical exams, provides immediate results, and is easily demonstrated to and performed by local healthcare providers. The straightforward and sensitive VIA technique is an ideal cervical cancer screening method for resource poor areas.

  2. 浙江沿海地区人群TTV和HPV混合感染研究%TTV and HPV co-infection in cervical smears of patients with cervical lesions in littoral of Zhejiang province

    Institute of Scientific and Technical Information of China (English)

    郑美云; 林颖; 李丹军; 阮海波; 陈怡; 吴婷婷

    2010-01-01

    目的 了解浙江沿海地区人群TTV与HPV混合感染状况,探讨TTV传播途径.方法 建立巢式聚合酶链反应方法(nPCR),对健康体检和患宫颈疾病妇女150例宫颈病变细胞学标本及其平行97例血清标本进行TTV DNA及TTV病毒滴度的nPCR检测;应用导流杂交方法检测95例宫颈病变细胞学标本的HPV基因型.结果 TTV DNA在55例健康妇女宫颈细胞标本中检出率52.7%(29/55),与其平行42例血清样本中检出率50.0%(21/42).在患有疾病妇女宫颈细胞中TTV DNA检出率(74.7%)高于健康体检对照组(P=0.005).TTV DNA在患者血清样本中检出率51%(28/55).在宫颈细胞及其平行血清中均检测出TTV基因亚型G1b°TTV病毒滴度在宫颈细胞中高于在其平行血清10~1000倍.HPV在患者组中检出率98.9%(94/95),在健康组中检出率27.3%(15/55).HPV基因型是高危型HPV16、18、33和低危型HPV6.HPV阳性标本TTV DNA检测率明显高于HPV阴性标本(P=0.02).结论 TTV在宫颈细胞中具有高检出率,在宫颈细胞中TTV病毒滴度高于其平行血清.TTV与HPV随性传播感染人群,并在女性生殖道内繁殖.TTV与HPV协同作用有待研究.%Objective To investigate the prevalence of transfusion-transmitted virus (TTV) and human papillomavirus (HPV) co-infection in cervical smears of patients with cervical lesions in littoral of Zhejiang province and analysis of transmitted route.Methods Nested polymerase chain reaction (nPCR) was established.TTV DNA were tested by nPCR in cervical smears of 95 patients with cervical lesions and 55 healthy women,paired serum samples were available from 55 and 42 women,and their viral titer.The genotypes of 95 specimens of cervical cytology were detected with HybriMax.The phylogenetic group of TTV was determined by means of nPCR with N22 primers.Results The prevalence of TTV DNA in cervical smears of patients with cervical lesions and healthy women was 52.7% (29/55) and was comparable with that in paired serum

  3. Clinical Value of LCT and HR-HPV in the Diagnosis of Cervical Lesions%LCT及HR-HPV诊断宫颈病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    李玉环; 戴红英; 闫丽萍; 丁朝霞

    2015-01-01

    Objective To study liquid-based cervical cytology and high risk human papilloma virus testing and bi⁃opsy in the diagnosis, discusses the cervical liquid based cytology and high-risk human papilloma virus detection value to the diagnosis of cervical lesions. Methods Retrospective analysis of the Affiliated Hospital of Qingdao University outpa⁃tient 20479 cases of LCT screening from January 2013 to January 2014. LCT positives were further investigated by HR-HPV testing and colposcopy cervical biopsy pathology, compare the accuracy of the LCT positive results and HR-HPV test results with cervical biopsy and colposcopy results with pathology detection results as the gold standard of diagnosis. Results 1)The consistence rates of ASCUS, LSIL, ASCH, HSIL by LCT and colposcopic multiple biopsies were 54.55%, 62.65%, 88.24%, 92.99%. HR- HPV infection rates of ASCUS, LSIL, ASCH and HSIL respectively were 70.00%, 88.71%, 84.91%, 89.66%. There was seriously statistical significance(P<0.001).2)HR-HPV positive and nega⁃tive groups compared with the high-level cervical lesion detection rates were 48.14%, 17.39%(P<0.001). There was se⁃riously statistical significance. Conclusion Liquid-based cytology and high-risk human papillomavirus detection is an effective screening method for detecting cervical lesions, higher diagnostic accuracy of both joint detection. High-risk hu⁃man papillomavirus detection for cytologic diagnosis of atypical squamous cells of patients has significant shunt value.%目的:探讨LCT和HR-HPV对宫颈病变的诊断价值。方法对20479例患者行LCT检查,LCT阳性者行HR-HPV检测及电子阴道镜下宫颈活检组织病理检查,以组织病理学结果作为诊断的金标准,比较LCT阳性结果、HR-HPV检测结果与阴道镜下宫颈活检病理结果的符合率。结果1)LCT结果为ASCUS、LSIL、ASCH、HSIL与阴道镜下宫颈活检病理结果的符合率分别为54.55%、62.65%)、88.24%、92.99%

  4. 人乳头瘤病毒衣壳蛋白与宫颈病变%Human Papillomavirus′ Capsid Proteins and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    黄成琳; 张淑兰

    2014-01-01

    宫颈癌严重危害妇女健康,人乳头瘤病毒(HPV)感染是其首要病因。临床医师一直致力于寻找一种能有效判断宫颈病变级别及预测预后的诊断方法。 HPV衣壳蛋白包括主要衣壳蛋白(L1壳蛋白)和次要衣壳蛋白(L2壳蛋白),这两种蛋白在组装成病毒颗粒、协助病毒入胞及引起机体免疫反应等多个方面发挥重要作用。近年研究表明, L1壳蛋白可用于预测宫颈病变的进展与消退。以L1及L2壳蛋白为基础研发的HPV预防性疫苗在临床试验中得到了很好的预防效果。综述HPV生物学特点及近年来有关HPV衣壳蛋白在宫颈病变的研究进展。%Cervical cancer seriously endangers women′s health,and human papillomavirus (HPV) is considered to be the primary cause. Doctors have been striving to find an effective diagnostic method for judging cervical lesions level and predicting its prognosis. HPV capsid proteins comprise the major capsid protein (L1 capsid protein) and the minor capsid protein (L2 capsid protein),and these two proteins play an important role in assembling into virus particles,trafficking HPV to the cell,and causing the host′s immune reactions. In recent years,studies have shown that the L1 capsid protein can be used to predict the progress and subsidence of cervical lesions. HPV prophylactic vaccines ,which are exploited on the basis of the L1 and L2 capsid protein,are proved to get a good preventive effect in clinical trials. This paper reviews the biological characteristics of HPV and researches progress on HPV capsid protein in cervical lesions in recent years.

  5. 人乳头瘤病毒衣壳蛋白与宫颈病变%Human Papillomavirus′ Capsid Proteins and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    黄成琳; 张淑兰

    2014-01-01

    Cervical cancer seriously endangers women′s health,and human papillomavirus (HPV) is considered to be the primary cause. Doctors have been striving to find an effective diagnostic method for judging cervical lesions level and predicting its prognosis. HPV capsid proteins comprise the major capsid protein (L1 capsid protein) and the minor capsid protein (L2 capsid protein),and these two proteins play an important role in assembling into virus particles,trafficking HPV to the cell,and causing the host′s immune reactions. In recent years,studies have shown that the L1 capsid protein can be used to predict the progress and subsidence of cervical lesions. HPV prophylactic vaccines ,which are exploited on the basis of the L1 and L2 capsid protein,are proved to get a good preventive effect in clinical trials. This paper reviews the biological characteristics of HPV and researches progress on HPV capsid protein in cervical lesions in recent years.%宫颈癌严重危害妇女健康,人乳头瘤病毒(HPV)感染是其首要病因。临床医师一直致力于寻找一种能有效判断宫颈病变级别及预测预后的诊断方法。 HPV衣壳蛋白包括主要衣壳蛋白(L1壳蛋白)和次要衣壳蛋白(L2壳蛋白),这两种蛋白在组装成病毒颗粒、协助病毒入胞及引起机体免疫反应等多个方面发挥重要作用。近年研究表明, L1壳蛋白可用于预测宫颈病变的进展与消退。以L1及L2壳蛋白为基础研发的HPV预防性疫苗在临床试验中得到了很好的预防效果。综述HPV生物学特点及近年来有关HPV衣壳蛋白在宫颈病变的研究进展。

  6. Detection of Human Papilloma Virus (HPV in oral mucosa of women with cervical lesions and their relation to oral sex practices

    Directory of Open Access Journals (Sweden)

    Martinez-Martinez Alejandro

    2010-12-01

    Full Text Available Abstract Background Previous studies have either investigated the relationship of HPV with oral cancer or the prevalence of HPV on the oral cavity. The purpose of this investigation was to study the prevalence of HPV in oral cavity of women with oral sex practices and cervical lesions. Methods Forty six (46 non-smokers and non-alcoholic patients attended the "Clínica de Displasias" of "Ciudad Juarez" were sampled. This population had a CIN diagnosis sometime between the previous six months. On previous consent they filled out a questionnaire related to their oral sex practices. Afterwards one swab from cheeks and another from palate/gum were taken; PCR was used to determine generic HPV, HPV16 and HPV18. Results Seventy two percent (72% of the patients stated to have oral sex practices regularly which all of them were positive to HPV either in oral mucus, palate/gum or both. The total of the given results showed that 35% had HPV16; among those distributed in 26% with regular oral sex practices and 9% stated as never practiced oral sex. An association was found between oral HPV16 positivity and progression to cervical CIN advanced lesions. On the other hand HPV18 was not detected. The frequency of HPV16 was higher in buccal mucosa (23% versus palate/gum (16%. Conclusions This study suggests that buccal HPV16 infection is associated with CIN progression.

  7. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion

    Science.gov (United States)

    de Abreu, André LP; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria DB; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia EL

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  8. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.

    Science.gov (United States)

    de Abreu, André Lp; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria Db; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia El

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  9. The SNP at −592 of human IL-10 gene is associated with serum IL-10 levels and increased risk for human papillomavirus cervical lesion development

    Directory of Open Access Journals (Sweden)

    Torres-Poveda Kirvis

    2012-11-01

    Full Text Available Abstract Background Women with Human Papilloma Virus (HPV persistence are characterized by high levels of IL-10 at cervix. We have determined whether polymorphisms of IL-10 gene promoter might be associated with increased risk of squamous intraepithelial cervical lesions (SICL and whether exist significative differences of IL-10 mRNA expression at cervix and systemic and serum IL-10 protein between SICL cases and non-Cervical Lesions (NCL. Methods Peripheral blood samples from SICL (n = 204 and NCL (n = 166 were used to detect IL-10 promoter polymorphisms at loci -592A/C (rs1800872, -819C/T (rs1800871, -1082A/G (rs1800896, -1352A/G (rs1800893, by allelic discrimination and to evaluate serum IL-10 protein. Cervical epithelial scrapings from NCL and biopsies from SICLs were used for HPV-typing and to evaluate IL-10 mRNA expression level. The systemic and local IL-10 mRNA expression levels were measured by real time-PCR. Genotypic and allelic frequencies of the selected polymorphisms were analyzed by logistic regression, adjusting by age and HPV-genotype, to determine the association with SICL. Results No significant differences were found between genotype frequencies at loci −819, -1082, and −1352. Individuals carrying at least one copy of risk allele A of polymorphism −592 had a two-fold increased risk of developing SICL [adjusted odds ratio (OR, 2.02 (95% CI, 1.26-3.25, p = 0.003], compared to NCL. The IL-10 mRNA expression and serum IL-10 protein, were significantly higher in SICL cases (p  Conclusions The −592 polymorphism is associated with increased risk of SICL and can serve as a marker of genetic susceptibility to SICL among Mexican women. According to IL-10 levels found in SICL, IL-10 can be relevant factor for viral persistence and progression disease.

  10. Clinical value of TCT combined with HPV-DNA detection in cervical cancer screening%液基薄层细胞学联合 HPV-DNA检测对宫颈癌患者筛查的临床价值

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2016-01-01

    Objective:To explore clinical value ThinPrep cytology test ( TCT) and HPV detection in cervical cancer screen-ing. Methods:The clinical data of the patients in the screening of cervical precancerous lesions were collected. Each patient was given TCT combined with HPV detection. Finally, after colposcopy cervical biopsy, the sensitivity, accuracy and false positive rate of single TCT, single HPV and TCT combined with HPV in the diagnosis of chronic cervical inflammation, cervical precancerous lesions and cervical cancer were compared. Results:The cervical cancer and precancerous lesions screening under colposcopy biopsy was done in 360 patients. Among the 3 screening methods, the combined application of TCT and HPV had the highest sensitivity and specificity, and could be used for diagnosing severe atypical hyperplasia ( CIN-III) and cervical carcinoma without missed diagnosis;however, it might miss some moderate dysplasia ( CIN-II) and mild atypical hyperplasia ( CIN-I) patients. Conclusions:The TCT combined with HPV-DNA detection can be used actively in the cervical precancerous lesions screening, and improve the accuracy of clinical diagno-sis. Moreover, combined with colposcopy biopsy of cervical cancer and precancerous lesions, it can provide a diagnostic basis for the clinical treatment, and is worthy of clinical application.%目的::探讨液基薄层细胞学与HPV检测联合应用后在宫颈癌患者筛查中的临床应用价值。方法:分析行宫颈癌前病变筛查的患者共360例临床资料,每位患者给予薄层液基细胞学联合HPV检测,最后经阴道镜下宫颈活检,比较单独TCT检查、单独HPV检查及两种检查联合检测在诊断患者宫颈慢性炎症、宫颈癌前病变及宫颈癌的敏感程度、准确性和假阳性率。结果:宫颈癌及癌前病变筛查患者,行阴道镜下活检的患者360例。3种筛选方法中,联合应用TCT及HPV的敏感性、特异性最

  11. The association between methylated CDKN2A and cervical carcinogenesis, and its diagnostic value in cervical cancer: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Li J

    2016-08-01

    Full Text Available Jinyun Li,1,2,* Chongchang Zhou,1,* Haojie Zhou,3,* Tianlian Bao,1 Tengjiao Gao,1 Xiangling Jiang,1 Meng Ye1,2 1Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, 2Department of Medical Oncology, Affiliated Hospital, Ningbo University, 3Department of Molecular Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, Zhejiang, People’s Republic of China *These authors are co-first authors of this work Background: Cervical cancer is the second deadliest gynecologic malignancy, characterized by apparently precancerous lesions and cervical intraepithelial neoplasia (CIN, and having a long course from the development of CIN to cervical cancer. Cyclin-dependent kinase inhibitor 2A (CDKN2A is a well-documented tumor suppressor gene and is commonly methylated in cervical cancer. However, the relationship between methylated CDKN2A and carcinogenesis in cervical cancer is inconsistent, and the diagnostic accuracy of methylated CDKN2A is underinvestigated. In this study, we attempted to quantify the association between CDKN2A methylation and the carcinogenesis of cervical cancer, and its diagnostic power.Methods: We systematically reviewed four electronic databases and identified 26 studies involving 1,490 cervical cancers, 1,291 CINs, and 964 controls. A pooled odds ratio (OR with corresponding 95% confidence intervals (95% CI was calculated to evaluate the association between methylated CDKN2A and the carcinogenesis of cervical cancer. Specificity, sensitivity, the area under the receiver operating characteristic curve, and the diagnostic odds ratio were computed to assess the effect of methylated CDKN2A in the diagnosis of cervical cancer.Results: Our results indicated an upward trend in the methylation frequency of CDKN2A in the carcinogenesis of cervical cancer (cancer vs control: OR =23.67, 95% CI =15.54–36.06; cancer vs CIN: OR =2.53, 95% CI =1.79–3.5; CIN vs control: OR =9.68, 95% CI =5.82–16.02. The

  12. Study on correlation of pathological changes of chronic atrophic gastritis gastric mucosal precancerous lesions with TCM syndrome type and TRPV1, TRPM8%慢性萎缩性胃炎胃黏膜癌前病变病理变化与中医证型及 TRPV1、TRPM8的相关性研究

    Institute of Scientific and Technical Information of China (English)

    王俊; 黄雅慧

    2014-01-01

    Objective It is to observe the correlation of pathological changes of chronic atrophic gastritis ( CAG) precan-cerous lesions with TCM syndrome type and transient receptor potential (TRPV1, TRPM8), and provide more objective basis of TCM syndrome differentiation for CAG .Methods317 patients with CAG diagnosed by endoscopy and CAG with atypical hy-perplasia or different levels intestinal metaplasia diagnosed by pathology were selected ;10 cases with asymptomatic superficial gastritis found by regular physical examination were selected as a control .The detection of TRPV1 receptors , TRPM8 expres-sion and the CAG syndrome types of TCM syndrome differentiation were done , and the correlation of pathological changes of CAG with TCM syndrome type and the correlation of TRPV 1, TRPM8 expression were analyzed ,Results①The TCM syndrome type of CAG precancerous lesions were accorded to the sort of spleen deficiency and Qi stagnation >Spleen stomach damp heat >weakness of spleen and stomach >Liver stomach disharmony >Stomach yin deficiency >stomach collaterals stasis resistance .②There were significant difference in the expression of TRPV 1 and TRPM8 between the six TCM syndrome types . The syndrome types were sorted to Spleen stomach damp heat >Stomach yin deficiency >Liver stomach disharmony >stom-ach collaterals stasis resistance >spleen deficiency and Qi stagnation >weakness of spleen and stomach for TRPV 1 expres-sion and sorted to weakness of spleen and stomach >spleen deficiency and Qi stagnation >stomach collaterals stasis resist-ance >Liver stomach disharmony >Stomach yin deficiency >Spleen stomach damp heat for TRPM 8 expression .TRPV1 was expressed obviously in Spleen stomach damp heat type at 415 bp, TRPM8was expressed obviously in weakness of spleen and stomach type at387 bp.stomach collaterals stasis resistance type was similar the expression of superficial gastritis gastric mu -cosa.③There was significant difference in the same syndrome among different

  13. Correlation of DNA Ploidy with Progression of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    M. Singh

    2008-01-01

    Full Text Available The majority of squamous cell carcinomas of cervix are preceded by visible changes in the cervix, most often detected by cervical smear. As cervical cancer is preceded by long precancerous stages, identification of the high-risk population through detection of DNA ploidy may be of importance in effective management of this disease. Here we attempted to correlate aneuploid DNA patterns and their influence on biological behavior of flow-cytometry analysis of DNA ploidy which was carried out in cytologically diagnosed cases of mild (79, moderate (36, and severe (12 dysplasia, as well as “atypical squamous cells of unknown significance (ASCUS” (57 along with controls (69, in order to understand its importance in malignant progression of disease. Cytologically diagnosed dysplasias, which were employed for DNA ploidy studies, 39 mild, 28 moderate, and 11 severe dysplasia cases were found to be aneuploid. Out of the 69 control subjects, 6 cases showed aneuploidy pattern and the rest 63 subjects were diploid. An aneuploidy pattern was observed in 8 out of 57 cases of cytologically evaluated ASCUS. The results of the followup studies showed that aberrant DNA content reliably predicts the occurrence of squamous cell carcinoma in cervical smear. Flow cytometric analysis of DNA ploidy may provide a strategic diagnostic tool for early detection of carcinoma cervix. Therefore, it is a concept of an HPV screening with reflex cytology in combination with DNA flow cytometry to detect progressive lesions with the greatest possible sensitivity and specificity.

  14. HPV infection subtype distribution and its relation with cervical lesions%HPV 感染亚型分布及其与宫颈病变关系的研究

    Institute of Scientific and Technical Information of China (English)

    段山红; 安瑞芳

    2015-01-01

    among gynecological outpatients in Xianyang district and its relation with cervical lesions.Methods Between January 2009 and February 2013, totally 3 573 outpatients undergoing HPV genotyping and liquid-based cytology check were selected in three tertiary hospitals in Xianyang district.There were 966 cases with HPV genotype positive ( including 19 kinds of high-and low-risk subtype) and 572 cases with cytological positive ( ASCUS) .Both positive results were found in 354 cases.For 1 169 patients with any positive results, colposcopy and cervical biopsy were performed.The correlation between subtypes of HPV infection and cervical lesions was analyzed according to pathological examination results.Results HPV testing positive rate was 27.04%.HR-HPV positive rate was 24.55%, and HPV16 positive rate was 12.26% accounting for 45.34% of HR-HPV.The highest positive rate of HPV was found among women aged below 30.With age increasing, HPV positive rate declined gradually.There were significant differences in HPV positive rate among different age groups except for group aged≤20 years and group aged 21-30 years (χ2 value was 5.08, 8.93, 37.48 and 4.28, respectively, all P<0.05).The detection rates of HPV16 in CINⅡ, CINⅢand squamous cell cancer (SCC) were highest, and they were 50.59%, 78.13%and 81.82%, respectively.Compared with HPV58, HPV18, HPV52 and HPV33, HPV16 caused CINⅡor above more easily, and the difference was statistically significant (χ2 value was 53.93,62.84,93.01 and 93.01, respectively, all P<0.05).Conclusion The most common high-risk HPV genotypes infected in outpatients in Xianyang district are HPV16, HPV52, HPV58, HPV18 and HPV33.HPV16 plays a key role in the genesis and development of cervical cancer and precancerous lesions.HPV58, HPV18, HPV52, and HPV33 are also pathogenic in this area.High-risk HPV genotyping is therefore very important in cervical cancer screening and the process of prevention and diagnosis.

  15. Characterization of High Grade Intraepithelial Cervical Lesion among Adolescents and Young Women Caracterización de las lesiones intra-epiteliales cervicales de alto grado en adolescentes y mujeres jóvenes

    Directory of Open Access Journals (Sweden)

    Carlos Eric Daudinot Cos

    2011-11-01

    Full Text Available Introduction: considering the relationship between human papilomavirus and cervical cancer, the current increase in sexually transmitted diseases constitutes the biggest threaten to adolescents’ health. Objective: to characterize high-degree intraepithelial cervical lesion in adolescents and young women. Method: a descriptive and observational study was conducted. It included 52 patients, all of them younger than 24 years old, who had been diagnosed with high-degree cervical pathology and were treated in the cervix pathology consultation of the "Ramón González Coro" Teaching Gynecologic and Obstetric Hospital from January 2007 to December 2008. Risk factors and diagnosis variables were studied and processed using the SPSS 11.5 system for Windows, through the determination of absolute and relative frequencies. Results: 78,8 % of patients had from 20 to 24 years old, 65,3 % of them began having sexual relationships between 15 and 17 years old, 62,2 % had had 3 or more sexual partners and 67,3 % used no contraceptive method. Cyto- histological correlation was of 100 % for intraepithelial cervical lesion II and III. Conclusion: the results of the present study compromise us to highlight the need of extended sexual education among young people.Introducción: el actual incremento de las Infecciones de Transmisión Sexual constituye la mayor amenaza para la salud de los adolescentes, si se conoce la asociación del Virus del Papiloma Humano con el cáncer cervical. Objetivo: caracterizar las lesiones intra-epiteliales cervicales de alto grado en adolescentes y mujeres jóvenes. Método: estudio observacional, descriptivo, en 52 pacientes menores de 24 años con diagnostico de lesión cervical de alto grado, atendidas en la consulta de patología de cuello del Hospital Ginecobstétrico ¨Ramón González Coro¨, de

  16. ANTIGEN MG7 IN GASTRIC CANCER AND GASTRIC PRECANCEROUS LESIONS

    Institute of Scientific and Technical Information of China (English)

    郭冬丽; 宁佩芳; 袁媛

    2004-01-01

    Objective: To study the dynamic change and its diagnostic significance of MG7 expression in the process of gastric cancer development. Methods: The expression level of antigen MG7 was determined by immunohistochemistry method in 406 cases of gastric mucosa. The classification of intestinal metaplasia of gastric mucosa was determined by histochemistry method in 82 cases. Results: The positive rate of MG7 expression in normal gastric mucosa, intestinal metaplasia and dysplasia of gastric mucosa and gastric cancer were increased gradually (P<0.01). The positive rate of MG7 expression in superficial gastritis, atrophic gastritis and gastric cancer were increased on sequence (P<0.01). The positive rate of antigen MG7 expression in type Ⅲ intestinal metaplasia of gastric mucosa had significant difference,compared with that in type Ⅰ an Ⅱ intestinal metaplasia (P<0.05). Conclusion: MG7 antigen had close relationship with gastric cancer. Type Ⅲ intestinal metaplasia, atrophic gastritis and dysplasia should be followed up in order to improve the early detection of gastric cancer. MG7 antigen had great clinical value in the dynamic follow-up of gastric precursors.

  17. Mitochondrial microsatellite instability in gastric cancer and its precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    Xian-Long Ling; Dian-Chun Fang; Rong-Quan Wang; Shi-Ming Yang; Li Fang

    2004-01-01

    AIM: To evaluate the role of mitochondrial microsatelliteinstability (mtMSI) in gastric carcinogenesis. METHODS: MtMSI was measured with PCR-single strand conformation polymophism (PCR-SSCP) in 68 cases of advanced gastric cancer, 40 cases of chronic gastritis, 30 cases of intestinal metaplasia and 20 cases of dysplasia.RESULTS: MtMSI was observed in 12.5% (5 of 40) of chronic gastritis, 20.0% (6 of 30) of intestinal metaplasia, 25.0% (5 of 20) of dysplasia and 38.2% (26 of 68) of gastric cancer. These findings showed a sequential accumulation of mtMSI in the histological progression from chonic gastritis to gastric cancer. An association of mtMSI with intestinal histological type and distal location was found (P=0.001 and P=0.002), whereas no significant correlation was found between mtMSI and age at diagnosis, sex, tumor size, depthof invasion, lymph node spread and clinical stages (P>0.05).CONCLUSION: MtMSI may play an early and importantrole in the gastric carcinogenesis pathway, especially in the intestinal type and distal gastric cancer.

  18. Early Detection of Oral Pre-Cancerous Lesions: Recent Advances

    Directory of Open Access Journals (Sweden)

    Anita Meena

    2012-07-01

    Full Text Available The high mortality rate in cancer such as oral squamous cell carcinoma is commonly attributed to the negligence in detection of the disease at an early treatable stage. A number of promising recent technologies have been proposed to improve the effectiveness of early oral cancer detection. The goal of a cancer-screening is to detect tumors at a stage early enough so that treatment is likely to be successful. Screening tools are needed that exhibit the combined features of high sensitivity and specificity. Moreover, the screening tool must be sufficiently noninvasive and inexpensive to allow widespread applicability. This paper will attempt to overview the recent advances in the dentistry with special emphasis on detection of oral cancer in early stages and also aim to familiarize, the various diagnostic tools among undergraduates’ and postgraduates’ from different medical fields.

  19. Pre-cancerous (DNA and chromosomal lesions in professional sports

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    Radhika Sharma

    2012-01-01

    Conclusion: Significantly increased genomic instability in players of both sports was observed. Both repaired and repairable genetic damage cells were observed in different tissues of the same subject. The presence of such genetic damage implies that these players are at an individual risk from cancer- and age-related diseases.

  20. Are histological alterations observed in the gallbladder precancerous lesions?

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    Adriana Lúcia Agnelli Meirelles-Costa

    2010-01-01

    Full Text Available INTRODUCTION: Gallbladder cancer, which is characterized by rapid progression and a poor prognosis, is a complex disease to treat. Unfortunately, little is known currently about its etiology or pathogenesis. A better understanding of its carcinogenesis and determining risk factors that lead to its development could help improve the available treatment options. METHOD: Based on this better understanding, the histological alterations (such as acute cholecystitis, adenomyomatosis, xanthogranulomatous cholecystitis, polyps, pyloric metaplasia, intestinal metaplasia, dysplasia, cancer and others in gallbladders from 1,689 patients who underwent laparoscopic cholecystectomy for cholecystolithiasis were analyzed. The association of these gallbladder histological alterations with clinical data was studied. RESULTS: Gender analysis revealed a greater incidence of inflammatory changes in males, while dysplasia and cancer were only found in women. The incidence of cholesterolosis was greater in the patients 60 years of age and under, and the incidence of adenomyomatosis and gangrene was greater in the elderly patients. A progressive increase in the average age was observed as alterations progressed through pyloric metaplasia, intestinal metaplasia, dysplasia and then cancer, suggesting that the metaplasia-dysplasia-carcinoma sequence may occur in gallbladder cancer. Gallbladder histological alterations were also observed in asymptomatic patients. CONCLUSION: The results of this study suggest that there could be an association between some histological alterations of gallbladder and cancer, and they also suggest that the metaplasia-dysplasia-carcinoma sequence could in fact be true in the case of gallbladder cancer. Nevertheless, further studies directed towards a perfect understanding of gallbladder carcinogenesis are required.

  1. Targeting immune response with therapeutic vaccines in premalignant lesions and cervical cancer: hope or reality from clinical studies.

    Science.gov (United States)

    Vici, P; Pizzuti, L; Mariani, L; Zampa, G; Santini, D; Di Lauro, L; Gamucci, T; Natoli, C; Marchetti, P; Barba, M; Maugeri-Saccà, M; Sergi, D; Tomao, F; Vizza, E; Di Filippo, S; Paolini, F; Curzio, G; Corrado, G; Michelotti, A; Sanguineti, G; Giordano, A; De Maria, R; Venuti, A

    2016-10-01

    Human papillomavirus (HPV) is widely known as a cause of cervical cancer (CC) and cervical intraepithelial neoplasia (CIN). HPVs related to cancer express two main oncogenes, i.e. E6 and E7, considered as tumorigenic genes; their integration into the host genome results in the abnormal regulation of cell cycle control. Due to their peculiarities, these oncogenes represent an excellent target for cancer immunotherapy. In this work the authors highlight the potential use of therapeutic vaccines as safe and effective pharmacological tools in cervical disease, focusing on vaccines that have reached the clinical trial phase. Many therapeutic HPV vaccines have been tested in clinical trials with promising results. Adoptive T-cell therapy showed clinical activity in a phase II trial involving advanced CC patients. A phase II randomized trial showed clinical activity of a nucleic acid-based vaccine in HPV16 or HPV18 positive CIN. Several trials involving peptide-protein-based vaccines and live-vector based vaccines demonstrated that these approaches are effective in CIN as well as in advanced CC patients. HPV therapeutic vaccines must be regarded as a therapeutic option in cervical disease. The synergic combination of HPV therapeutic vaccines with radiotherapy, chemotherapy, immunomodulators or immune checkpoint inhibitors opens a new and interesting scenario in this disease.

  2. PA03.11. Evaluation of socioeconomic status and other factors with special reference to cervical cancer A survey based study

    OpenAIRE

    Panwar, Aruna; ,

    2013-01-01

    Purpose: Cervical cancer is cancer that starts in the cervix, the lower part of the uterus (womb) that opens at the top of the vagina. It is the third most common type of cancer in women. Cervical cancers start in the cells on the surface of the cervix. Most cervical cancers are from squamous cells. It starts as a precancerous condition called dysplasia. This precancerous condition can be detected by a Pap smear and is 100% treatable. It can take years for precancerous changes to turn into ce...

  3. Risk of cervical cancer and precancerous lesions in patients infected with human papilloma virus%人乳头状瘤病毒感染致宫颈癌及癌前病变风险研究

    Institute of Scientific and Technical Information of China (English)

    严敏婵; 张晓雪; 陈东; 陈星; 袁兆林; 卢洪胜; 沈波

    2010-01-01

    目的 探讨感染人乳头状瘤病毒(human papilloma virus,HPV)后患宫颈癌及癌前病变风险.方法 采用HPV-DNA核酸扩增及导流杂交技术,对7 234例来我院就诊的宫颈异常患者,进行HPV21种亚型的基因检测和宫颈液基薄层细胞学(TCT)检测,对细胞学检测结果为非典型鳞状细胞(ASCUS)以上和/或HPV-DNA检测阳性的患者,行活组织病理检查,对HPV感染后患宫颈癌及癌前病变风险进行分析.结果 HPV总感染率为27.37%,检出率前四位依次是HPV 16型6.97%(504/7 234)、52型5.68%(411/7 234)、58型5.27%(381/7 234)和33型3.33%(241/7 234),其中高危型病毒(HR-HPV)感染占78.16%(2147/2747).宫颈癌患者HPV的检出率为73.33%(33/45).单型HPV感染后患轻度鳞状上皮内瘤变(LSIL)及高度鳞状上皮内瘤变(HSIL)和宫颈癌的风险分别是HPV阴性者的4.055倍(95% CI 2.528~6.505)和5.103倍(95% CI 3.838~6.786),多型混合HPV感染后患宫颈LSIL及HSIL和宫颈癌的风险分别是HPV阴性者的7.313倍(95% CI 4.314~12.397)和7.550倍(95%CI 5.395~10.564).结论 单型HPV感染致宫颈HSIL或宫颈癌的风险是HPV阴性者的5.1倍,多型混合HPV感染致宫颈HSIL或宫颈癌的风险是HPV阴性者的7.6倍.

  4. Expression of Pepsinogen C in Gastric Cancer and Precancerous Diseases and its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    PeifangNing; UpingSun; HuijieLiu; YuanYuan

    2004-01-01

    OBJECTIVE To investigate the active expression of pepsinogen C (PGC) and its value in detection of precancerous diseases and gastric cancer.METHODS Immunohistochemistry was used to examine the expression of pepsinogen C in 424 specimens of gastric mucosa collected by gastroscopy.RIFSULTS The positive rate of PGC expression in 54 cases of normal gastric mucosa was 100 % and 2.4% in 124 cases of gastric cancer. The positive rate of PGC expression in superficial gastritis, gastric ulcer or erosion, atrophic gastritis or gastric dysplasia and gastric cancer decreased significantly in the sequence indicated (P<0.05).CONCLUSION The expression of PGC is negatively correlated with the degree of malignancy of gastric mucosa and with development of gastric lesions. PGC expression has a high sensitivity and specificity for diagnosis of precancerous diseases which can lead to gastric cancer and may be a good indicator for screening and diagnosis of gastric cancer and precursors of gastric cancer.

  5. Investigation of non-carious cervical lesions of populations in Guangzhou area%广州市中高收入人群楔状缺损调查分析

    Institute of Scientific and Technical Information of China (English)

    闫文娟

    2012-01-01

    Objective To survey the prevalence, characteristics of non-carious cervical lesion and analyze its relationship with life style. Methods Eight hundred and eighty-five subjects including 490 males and 395 females who underwent oral health assessment in Nanfang hospital were surveyed. The shape, size, location of non-carious cervical lesion , tooth brushing habits, hand preference and bruxism were recorded for analysis. Results Non-carious cervical lesions were found in 76. 61% of subjects without gender difference. 70.17% affected teeth demonstrated v-shape lesion. Non-carious cervical lesions were mostly on posterior maxillary teeth and first premolars (34.29% ) were mostly affected. Non-carious cervical lesions were more common in right-hand tooth brusher. Senior patients were more likely to have non carious cervical lesions. Conclusion The prevalence of non-carious cervical lesions in Guangzhou area was high and the knowledge on this disease need to be improved.%目的 调查广州市部分中高收入人群的牙颈部楔状缺损患病率、病变特征及病因,为制定楔状缺损防治措施提供依据.方法 对885名中高收入人员楔状缺损状况进行调查,以楔状缺损牙数、缺损形状、好发牙位、刷牙方式等情况作为统计指标,对所得数据进行统计学分析.结果 该人群楔状缺损患病率为76.61%,男女差异无统计学意义(P>0.05);70.17%的楔状缺损为V形,主要发生在上颌后牙,以上颌第一前磨牙最常见,患病率为34.29%.年龄越大、习惯横向刷牙及爱吃硬食的人,楔状缺损患病率更高.调查人群中楔状缺损的就诊率为20.64%.结论 广州市部分中高收入人群的楔状缺损患病率较高,有喜欢吃硬食、横向刷牙等不良习惯的人群患病率更高.

  6. Risk factors of neurological lesions in low cervical spine fractures and dislocations Fatores de risco de lesão neurológica nas fraturas e luxações da coluna cervical baixa

    Directory of Open Access Journals (Sweden)

    DANILO GONÇALVES COELHO

    2000-12-01

    Full Text Available Eighty-nine patients with lower cervical spine fractures or dislocations were evaluated for risk factors of neurological lesion. The age, sex, level and pattern of fracture and sagittal diameter of the spinal canal were analysed. There were no significant differences on the age, gender, level and Torg's ratio between intact patients and those with nerve root injury, incomplete or complete spinal cord injuries. Bilateral facet dislocations and burst fractures are a significant risk factor of spinal cord injury.O risco de lesão neurológica foi avaliado em 89 pacientes com fraturas ou luxações da coluna cervical baixa. Foram analisados o sexo, a idade, o diâmetro sagital do canal vertebral, o nível e o tipo de fratura ou luxação. Não foram encontradas diferenças significativas para a idade, sexo, nível da fratura ou luxação e razão de Torg entre os pacientes intactos e aqueles com lesão radicular, lesão medular incompleta e lesão medular completa. Luxações bilaterais de facetas articulares e fraturas em explosão são um fator de risco significativo de lesão neurológica.

  7. Promoter hypermethylation-mediated inactivation of multiple Slit-Robo pathway genes in cervical cancer progression

    Directory of Open Access Journals (Sweden)

    Mansukhani Mahesh

    2006-05-01

    Full Text Available Abstract Background Cervical Cancer (CC exhibits highly complex genomic alterations. These include hemizygous deletions at 4p15.3, 10q24, 5q35, 3p12.3, and 11q24, the chromosomal sites of Slit-Robo pathway genes. However, no candidate tumor suppressor genes at these regions have been identified so far. Slit family of secreted proteins modulates chemokine-induced cell migration of distinct somatic cell types. Slit genes mediate their effect by binding to its receptor Roundabout (Robo. These genes have shown to be inactivated by promoter hypermethylation in a number of human cancers. Results To test whether Slit-Robo pathway genes are targets of inactivation at these sites of deletion, we examined promoter hypermethylation of SLIT1, SLIT2, SLIT3, ROBO1, and ROBO3 genes in invasive CC and its precursor lesions. We identified a high frequency of promoter hypermethylation in all the Slit-Robo genes resulting in down regulated gene expression in invasive CC, but the inhibitors of DNA methylation and histone deacetylases (HDACs in CC cell lines failed to effectively reactivate the down-regulated expression. These results suggest a complex mechanism of inactivation in the Slit-Robo pathway in CC. By analysis of cervical precancerous lesions, we further show that promoter hypermethylation of Slit-Robo pathway occurs early in tumor progression. Conclusion Taken together, these findings suggest that epigenetic alterations of Slit-Robo pathway genes (i play a role in CC development, (ii further delineation of molecular basis of promoter methylation-mediated gene regulation provides a potential basis for epigenetic-based therapy in advanced stage CC, and (iii form epigenetic signatures to identify precancerous lesions at risk to progression.

  8. Correlation of plasma nitrite/nitrate levels and inducible nitric oxide gene expression among women with cervical abnormalities and cancer.

    Science.gov (United States)

    Sowjanya, A Pavani; Rao, Meera; Vedantham, Haripriya; Kalpana, Basany; Poli, Usha Rani; Marks, Morgan A; Sujatha, M

    2016-01-30

    Cervical cancer is caused by infection with high risk human papillomavirus (HR-HPV). Inducible nitric oxide synthase (iNOS), a soluble factor involved in chronic inflammation, may modulate cervical cancer risk among HPV infected women. The aim of the study was to measure and correlate plasma nitrite/nitrate levels with tissue specific expression of iNOS mRNA among women with different grades of cervical lesions and cervical cancer. Tissue biopsy and plasma specimens were collected from 120 women with cervical neoplasia or cancer (ASCUS, LSIL, HSIL and invasive cancer) and 35 women without cervical abnormalities. Inducible nitric oxide synthase (iNOS) mRNA from biopsy and plasma nitrite/nitrate levels of the same study subjects were measured. Single nucleotide polymorphism (SNP) analysis was performed on the promoter region and Ser608Leu (rs2297518) in exon 16 of the iNOS gene. Differences in iNOS gene expression and plasma nitrite/nitrate levels were compared across disease stage using linear and logistic regression analysis. Compared to normal controls, women diagnosed with HSIL or invasive cancer had a significantly higher concentration of plasma nitrite/nitrate and a higher median fold-change in iNOS mRNA gene expression. Genotyping of the promoter region showed three different variations: A pentanucleotide repeat (CCTTT) n, -1026T > G (rs2779249) and a novel variant -1153T > A. These variants were associated with increased levels of plasma nitrite/nitrate across all disease stages. The higher expression of iNOS mRNA and plasma nitrite/nitrate among women with pre-cancerous lesions suggests a role for nitric oxide in the natural history of cervical cancer. PMID:26435258

  9. Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil

    Directory of Open Access Journals (Sweden)

    Geni NL Camara

    2003-10-01

    Full Text Available As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061 were less frequent (= or < 2.5% each. The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.

  10. Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells.

    Science.gov (United States)

    Kumar, Devbrat; Basu, Soumya; Parija, Lucy; Rout, Deeptimayee; Manna, Sanjeet; Dandapat, Jagneshwar; Debata, Priya Ranjan

    2016-07-01

    Cervical cancer and precancerous lesions of the cervix continue to be a global health issue, and the medication for the treatment for chronic HPV infection so far has not been effective. Potential anticancer and anti HPV activities of two known phytochemicals, Curcumin and Ellagic acid were evaluated in HeLa cervical cancer cells. Curcumin is a natural compound found in the root of Curcuma longa plant and Ellagic acid a polyphenol found in fruits of strawberries, raspberries and walnuts. The combination of Curcumin and Ellagic acid at various concentrations showed better anticancer properties than either of the drug when used alone as evidenced by MTT assay. Besides this, Curcumin and Ellagic acid also restore p53, induce ROS formation and DNA damage. Mechanistic study further indicated that Curcumin and Ellagic acid show anti-HPV activity as evidenced by decrease in the HPV E6 oncoprotein on HeLa cells. PMID:27261574

  11. 人乳头状瘤病毒基因亚型与宫颈病变的关系%The Relationship between Human Papillomavirus Subgenotype and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    张淑群

    2014-01-01

    Objective To study the relationship between human papilloma virus ( HPV-DNA) subgenotype infection and severity of cervical lesions .Methods 140 cases of HPV infection were selected as the study object .Cervical secretions were taken for viral genotyping ,21 kinds of HPV-DNA subtype analysis was performed by rapid hybridization using nucleic acid mole-cule genotyping technology ,all subjects received lower cervical TCT detection (LCT)and colposcopical multiple biopsy pathology . Under pathological diagnosis ,patients were divided into with normal or inflammation group ,low-grade squamous intraepithelial le-sion (LSIL)group,high-grade squamous intraepithelial lesion (HSIL)group.The relationship between HPV infection and cervical lesions was analyzed according to distribution of HPV-DNA subgenotype .Results 16 kinds of high-risk HPV mRNA isoforms were detected.The common genetic subtypes were HPV-16,58,52,18,33,31,CP8304,and HPV-16 had the highest detection rate.HPV-16 detection rates of the normal or inflammation group ,LSIL group and HSIL group were 19.23%,23.53%,65.22%, detection rate of HPV-16 in HSIL group was significantly higher than those of the other 2 groups .5 kinds of low-risk HPV genetic subtypes were detected,and HPV-11 was the most common.Detection rate of multiple HPV infection in HSIL group was 54.35%, which was significantly higher than that of the other 2 groups,P<0.05,there had statistical significance .Conclusion HPV-16, 58,52,18,33,31,CP8304 infection are the most common in cervical lesions .HPV genotyping assay is of great value for the diag-nosis and prognosis of cervical lesions ,and it is worthy of clinical application .%目的:研究人乳头状瘤病毒基因亚型( HPV-DNA)感染情况及其与宫颈病变程度的关系。方法选取140例HPV感染患者作为临床研究对象。取患者宫颈分泌物作病毒分型,采用核酸分子快速杂交基因分型技术进行21种HPV-DNA亚型分析,所有研究对象均行宫

  12. 子宫颈CIN病变切缘阳性的临床病理因素分析%Clinical pathological factors of margin-positive in cervical CIN lesions

    Institute of Scientific and Technical Information of China (English)

    喇建英; 张凡

    2014-01-01

    Objective:to study the cervical CIN lesions related factors of the cone cutting after cutting edge posi-tive.Methods:A retrospective analysis was performed for vaginal mirror cervical biopsy diagnosis of cervical intraepi-thelial neoplasia(CIN)and accept the cervical cone cut method.All 4527 cases of patients,the patients'age,degree of pathological changes,lesions scope,operation method and whether menopause and the cervical cone cutting and cut-ting edge positive rate for single factor and multiple factors analysis.Results:Multiariable logistic regression analysis found that the operation method is the scope,degree of disease,menopausal status,pathological changes and the cut edge positive highly relevant factors,the relative risk of 7.8735,5.0352,4.1055,4.5571,respectively.Conclusion:Cervical lesion degree of serious,large range of lesions,postmenopausal is cervical LEEP cone cutting operation cut-ting edge positive risk factors ,in the cervical cone cutting operation method choice should attach importance to it.%目的:探讨子宫颈CIN病变锥切术后切缘阳性的相关因素。方法:回顾性分析经阴道镜子宫颈活检诊断为宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)并接受宫颈锥切术的患者4527例,对患者年龄、病变程度、病变范围、手术方式及是否绝经与子宫颈锥切术切缘阳性率作单因素和多因素分析。结果:多因素logistic回归分析发现,手术方式、病变程度、是否绝经、病变范围是与切缘阳性高度相关的因素,相对危险度分别为7.8735、5.0352、4.1055、4.5571。结论:宫颈病变程度严重、病变范围大、LEEP、绝经后是宫颈锥切术切缘阳性的高危因素,在宫颈锥切手术方式选择时应予以重视。

  13. Value of Cervical Smear Cytology for Cervical Cancer Screening%宫颈刮片脱落细胞学检查对宫颈癌筛选的价值

    Institute of Scientific and Technical Information of China (English)

    黄瑞虹

    2013-01-01

    Objective:To explore the clinical value of cervical smear cytology for cervical cancer screening.Methods:5963 cases of gynecological examination of women underwent the cervical scraping levy cytology and TCT detection,the positive rate was compared.The TCT test result showed positive or suspected positive underwent colposcopy sampling biopsy,and was compared with the results of TCT test.Results:The positive rate of TCT detection of cervical lesions was significantly higher than that in the control group(P<0.01).There were a total of 538 cases of positive or suspected positive subjects by TCT detected, 449 cases of cervical precancerous lesions detected by biopsy,the detection rate was 83.46%.Conclusion:TCT detection of cervical precancerous lesions can help to improve clinical detection rate,the more significant of TCT diagnosis of abnormalities,the detection rate of biopsy is higher.%  目的:探讨宫颈刮片脱落细胞学检查对宫颈癌筛查的临床价值。方法:5963例妇科体检妇女均行宫颈刮片脱落细胞学检查及TCT检测,比较阳性率;TCT检测结果为阳性或疑似阳性受检者行阴道镜取样病理活检,并与TCT检测结果比较。结果:TCT检测宫颈病变的阳性率明显高于对照组(P<0.01);TCT检测共发现阳性或疑似阳性受检者538例,病理活检共检出宫颈癌前病变449例,检出率83.46%。结论:TCT检测有利于提高宫颈癌前病变的临床检出率,TCT诊断的异常性越显著,病理活检的检出率越高。

  14. 胃癌及癌前病变中COX-2和NF-κBp65蛋白表达及其相关性研究%Study on the protein expression of COX-2 and of NF-κBp65 and its correlation in gastric cancer and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    刘小玉; 冯义朝

    2013-01-01

    目的 观察幽门螺杆菌(Hp)、NF-κBp65、COX-2在胃黏膜癌变过程中的表达,探讨其表达与胃癌发生、发展的关系.方法 用免疫组化方法测定病例中COX-2、NF-κBp65蛋白的表达,用亚甲兰染色检测Hp.结果 ①慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、不典型增生(DYS)的Hp感染率与慢性浅表性胃炎(CSG)相比差异有显著性(x2=6.94,x2=6.71,x2=10.60,P<0.01).胃癌(GC)的Hp感染率与CSG相比差异无显著性(x2=1.37,P>0.05).②CAG、IM、DYS的COX-2表达率与CSG相比差异有显著性(x2 =4.81,x2=5.96,x2=16.57,P<0.05).GC的COX-2表达率与CSG相比差异亦有显著性(x2=34.50,P<0.01).③CAG、IM、DYS的NF-kBp65表达率与CSG相比差异有显著性(x2=4.71,x2=9.02,x2=20.74,P<0.05).GC的NF-kBp65表达率与CSG相比差异亦有显著性(x2=30.71,P<0.01).结论 Hp感染诱导COX-2和NF-kB表达发生在胃癌形成的早期阶段.COX-2和NF-kBp65参与了胃癌的发生发展过程,这些可能为胃癌的早期诊断及临床判断预后提供理论依据.%Objective To observe the expression of NF-kBp65 and COX-2 in gastric cancer and precancerous lesions, and to investigate the relationship between NF-kBp65 COX-2 expression and the pathogenesis of gastric cancer. Methods Slides were used for HE and sp immunohistochemical staining or Methylene Blue staining, the latter was used to detect the expression of NFkBp65, COX-2 and Hp infection. Results NF-kB and COX-2 expression is highly related with Hp infection. The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). Conclusion The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). NF-kBp65 and COX-2 expression is highly related with Hp infection. NF-kBp65 and COX-2 play important roles in the development progression of gastric carcinoma and NF-kBp65 may promote the expression of COX-2.

  15. 经阴道彩色多普勒超声在诊断宫颈占位中的价值%Value of Transvaginal Color Doppler Ultrasound in the Diagnosis of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    李萍; 王丹; 李利琼

    2014-01-01

    Objective To investigate the value of transvaginal color Doppler ultrasound in the diagnosis of patients with cervical lesions. Methods 179 patients with suspected cervical lesions in our hospital from December 2010 to December 2013 were selected in this study. All cases received transvaginal color Doppler ultrasound. All cases were confirmed by operation and histopathological examination, including 114 cases of cervical lesion, and 65 cases of non cervical lesion. The value of transvaginal color Doppler ultrasound in diagnosis of cervical lesions was analyzed. Results The sensitivity, specificity, and accuracy of transvaginal color Doppler ultrasound were 98.2%, 98.5%and 98.3%respectively. 112 patients with cervical lesions diagnozed by transvaginal color Doppler ultrasound included 37 cases of cervical cancer, 23 cases of cervical myoma, 41 cases of cervical polyps, 10 cases of ubmucosal myoma of uterus and one case of cervical pregnancy. Conclusions Transvaginal color Doppler ultrasound is an effective method in diagnosis of cervical lesions, which has high sensitivity, specificity and accuracy. Transvaginal color Doppler ultrasound can provide scientific basis for diagnosis and treatment of patients, which is worthy of clinical application.%目的:探讨经阴道彩色多普勒超声在诊断宫颈占位中的价值。方法选取本院2010年12月至2013年12月疑似宫颈占位病变患者179例,均行经阴道彩色多普勒超声检查,后经手术及病理组织学检查确诊,宫颈占位病变患者114例,非宫颈占位病变患者65例,分析经阴道彩色多普勒超声在诊断宫颈占位中的价值。结果经阴道彩色多普勒超声检查的灵敏度、特异度、准确度分别为98.2%、98.5%、98.3%。经阴道彩色多普勒超声诊断的112例宫颈占位病变患者中,宫颈癌37例、宫颈肌瘤23例、宫颈息肉41例、黏膜下子宫肌瘤10例、宫颈妊娠1例。结论经阴道彩色多普勒超声

  16. Application research of HPV test in screening of cervical lesions%HPV检测在宫颈病变筛查中的应用价值研究

    Institute of Scientific and Technical Information of China (English)

    揭艳频

    2014-01-01

    目的:探讨HPV检测在宫颈病变筛查中的应用价值。方法选择2011年2月-2013年6月本院门诊及体检的500例女性HPV检测的患者,分别给予HPV的TCT和DNA检测,并于病理组织检查结果进行比较。结果随着病变等级的升高, HPV DNA感染率逐渐升高。 TCT检测的阳性率为15.4%,HPV DNA检测的阳性率为35.2%,病理检测的阳性率为19.6%;TCT检测的灵敏度为44.90%,HPV DNA检测的灵敏度为83.67%,两组间差异有统计学意义(P<0.05)。结论 HPV DNA检测的灵敏度高于TCT检测,但两者各有优势,在宫颈病变筛查中的应用价值较高。%Objective To explore the application of HPV test in screening of cervical lesions. Methods 500 cases of female pa-tients with HPV tested in our hospital outpatient service and the physical examination from February 2011 to June 2013were se-lected, they were given the HPV TCT and DNA tested respectively, the examination results was compared with histopathologic. Results With the increase of level of lesion, the HPV DNA infection rate increased. TCT detection positive rate was 15.4%, HPV DNA detection positive rate was 35.2%, the pathological d detection positive rate was 19.6%;TCT detection sensitivity of 44.90%, the HPV DNA sensitivity of 83.67%, the differences between the two groups was statistically significant (P<0.05). Conclusion HPV DNA detection sensitivity is higher than the TCT detection, but they have their own advantages and high application value in screening of cervical lesions.

  17. Diagnostic Concordance Characteristics of Oral Cavity Lesions

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    Ufuk Tatli

    2013-01-01

    Full Text Available Purpose. The objective of this study was to evaluate the diagnostic concordance characteristics of oral cavity lesions by comparing the clinical diagnosis of the lesions with the histopathologic diagnosis. Material and Method. A retrospective analysis was conducted on the patients, who were admitted with oral cavity pathology and underwent biopsy procedure between 2007 and 2011. The oral cavity lesions were classified into 6 different groups as odontogenic cysts, nonodontogenic cysts, odontogenic tumors, nonodontogenic tumors, malignant tumors, and precancerous lesions in accordance with the 2005 WHO classification. The diagnoses were also recategorized into 3 groups expressing prognostic implications as benign, precancerous, and malignant. The initial clinical diagnoses were compared with the histopathologic diagnoses. Data were analyzed statistically. Results. A total of 2718 cases were included. Histopathologic diagnosis did not match the clinical diagnosis in 6.7% of the cases. Nonodontogenic tumors and malignant tumors had the highest misdiagnosis rates (11.5% and 9%, resp., followed by odontogenic tumors (7.7%, precancerous lesions (6.9%, and odontogenic cysts (4.4%. Clinicians were excelled in diagnosis of benign and precancerous lesions in clinical setting. Conclusion. The detailed discordance characteristics for each specific lesion should be considered during oral pathology practice to provide early detection without delay.

  18. Apoptosis induction in Jurkat cells and sCD95 levels in women's sera are related with the risk of developing cervical cancer

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    Bravo-Cuellar Alejandro

    2008-04-01

    Full Text Available Abstract Background Currently, there is clear evidence that apoptosis plays an important role in the development and progression of tumors. One of the best characterized apoptosis triggering systems is the CD95/Fas/APO-1 pathway; previous reports have demonstrated high levels of soluble CD95 (sCD95 in serum of patients with some types of cancer. Cervical cancer is the second most common cancer among women worldwide. As a first step in an attempt to design a minimally invasive test to predict the risk of developing cervical cancer in patients with precancerous lesions, we used a simple assay based on the capacity of human serum to induce apoptosis in Jurkat cells. We evaluated the relationship between sCD95 levels and the ability to induce apoptosis in Jurkat cells in cervical cancer patients and controls. Methods Jurkat cells were exposed to serum from 63 women (20 healthy volunteers, 21 with cervical intraepithelial neoplasia grade I [CIN 1] and 22 with cervical-uterine carcinoma. The apoptotic rate was measured by flow cytometry using Annexin-V-Fluos and Propidium Iodide as markers. Serum levels of sCD95 and soluble CD95 ligand (sCD95L were measured by ELISA kits. Results We found that serum from almost all healthy women induced apoptosis in Jurkat cells, while only fifty percent of the sera from women with CIN 1 induced cell death in Jurkat cells. Interestingly, only one serum sample from a patient with cervical-uterine cancer was able to induce apoptosis, the rest of the sera protected Jurkat cells from this killing. We were able to demonstrate that elimination of Jurkat cells was mediated by the CD95/Fas/Apo-1 apoptotic pathway. Furthermore, the serum levels of sCD95 measured by ELISA were significantly higher in women with cervical cancer. Conclusion Our results demonstrate that there is a strong correlation between low levels of sCD95 in serum of normal women and higher apoptosis induction in Jurkat cells. We suggest that an analysis of

  19. Atypical glandular cells in conventional cervical smears: Incidence and follow-up

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    Knolle Ulrich

    2004-07-01

    Full Text Available Abstract Background Atypical glandular cells on cervical smears are often associated with clinically significant uterine lesions. The frequency and accuracy of AGC-NOS (i.e. atypical glandular cells, not otherwise specified diagnoses, regardless of the gland cell type or the degree of suspicion, and their outcome were investigated. Methods From January 1, 1990 to December 31, 1999 a total of 261 patients had an AGC-NOS diagnosis made by conventional cervical Papanicolaou smear interpretation representing 0.05% of all Pap-smears analyzed at the national level. 191 (73.2% patients had a subsequent histological examination, 8 samples were not representative by origin and were excluded. Results Out of 183 AGC-NOS diagnosed, 56.3% (103/183 were associated with tissue-proven precancerous and/or cancerous lesions, 44% being of endocervical and 56% of endometrial origin. 75% of all AGC-patients were asymptomatic. 66.7% (6/9 of the patients with subsequent invasive endocervical adenocarcinoma (AC and 56% (28/50 of those patients with invasive endometrial AC were without clinical symptoms. 3 patients out of 9 with an invasive endocervical AC were 35 years of age or less. 10.1% and 12.3% of all 'new' tissue-proven invasive endocervical or endometrial AC respectively recorded by the national Morphologic Tumour Registry (MTR were first identified by a cytological AGC-NOS diagnosis. Conclusion Our findings emphasize the importance of the cytological AGC-category even in the absence of a precise origin or cell type specification. 56% of the AGC-diagnoses being associated with significant cancerous or precancerous conditions, a complete and careful evaluation is required.

  20. p14ARF与子宫颈癌及癌前病变关系的研究进展%Research progress of p14ARF in cervical carcinoma and cervical intraepithelial neoplasia

    Institute of Scientific and Technical Information of China (English)

    马晓洁

    2014-01-01

    Cervical cancer is one of the most common gynecological malignancies and is the only one which has certain pathogenesis in all malignancies currently.p14ARF is one of the tumor suppressor gene discovered recently and highly expressed in almost all cervical cancer.p14ARF has high specificity and sensitivity and it is related to invasion and prognosis of cervical cancer.Therefore,p14ARF is an ideal maker that can be used to early diagnose,screening precancerous lesions and predict prognosis in cervical cancer.%子宫颈癌是妇科最常见的恶性肿瘤之一,是目前所有恶性肿瘤中唯一病因明确、可进行早期预防和治疗控制的疾病.p14ARF是新近发现的一种抑癌基因,几乎在所有的子宫颈癌及癌前病变中高表达,且具有较高的特异性和敏感性,与侵袭及预后密切相关.因此,p14ARF是一种理想的标志物,可用于辅助子宫颈癌及其癌前病变的临床筛查诊断及判断预后.

  1. Clinical significance of Thinprep cytology test in screening of cervical lesions%液基薄层细胞学在筛查宫颈病变中的临床意义

    Institute of Scientific and Technical Information of China (English)

    张江菊; 黄寅虎; 郭建新; 李力; 郑秀惠

    2013-01-01

    Objective:To explore the clinical significance of Thinprep cytology test (TCT) in screening of cervical lesions.Methods:TCT screening was conducted among 18 904 patients from outpatient department of Daping Hospital Affiliated to the Third Military Medical University from January to December in 2012,and 688 patients were found with cytological abnormalities (diagnosis of TBS system ≥ ASC-US),then 416 patients underwent colposcopy and biopsy ; the clinical significance of TCT in screening of cervical lesions was evaluated with pathological examination as gold standard.Results:A total of 416 patients were found with cytological abnormalities,including 195 patients with ASC,65 patients with LSIL,138 patients with HSIL,and 52 patients with cervical cancer; 128 patients with cervicitis,106 patients with cervical intraepithelial neoplasia (CIN) Ⅰ,78 patients with CIN Ⅱ,52 patients with CIN Ⅲ,and 52 patients with cervical invasive carcinoma (including one patient with cervical adenocarcinoma) were found by pathological examination; the coincidence rates of diagnosis between pathological examination and TCT were 48.21% (ASC),81.54% (LSIL),89.13% (HSIL),and 100.00% (cervical cancer),respectively; the sensitivity and specificity of TCT for cervical lesions (≥ CIN Ⅱ) were 90.66% and 76.07%,respectively.Conclusion:TCT can improve the detection rate of cervical lesions and it has a high concordance with the histopathological results with high sensitivity,TCT is an effective method for screening cervical lesions and missed diagnosis can be reduced.%目的:探讨液基薄层细胞学在筛查子宫颈病变中的临床意义.方法:对2012年1月~12月第三军医大学大坪医院18 904例门诊患者进行TCT筛查,发现细胞学异常(TBS系统诊断≥ASC-US)的患者688例,其中416例进行了阴道镜检查并取活检,以病理学为标准,评价TCT筛查子宫颈病变的临床意义.结果:细胞学异常的416例患者中,ASC 195例,LSIL65

  2. 内镜智能分光比色技术对早期食管癌及癌前病变的诊断价值%Flexible spectral imaging color enhancement for diagnosis of early esophageal carcinomas and precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    李艳霞; 余世界; 沈磊; 罗和生

    2012-01-01

    目的 探讨内镜智能分光比色技术(FICE)对早期食管癌及癌前病变的诊断价值.方法 257例食管可疑病变患者分别接受FICE染色内镜、FICE染色放大内镜、2% Lugol液染色内镜、2% Lugol液染色放大内镜检查,并将内镜检查结果与活检病理结果进行对比分析.结果 FCIE染色内镜诊断早期食管癌的阳性率为92.6%(25/27),Lugol液染色内镜诊断早期食管癌的阳性率为88.9% (24/27),两者比较差异无统计学意义(P=0.642);FICE染色放大内镜诊断早期食管癌的阳性率为96.3%(26/27),Lugol染色放大内镜诊断早期食管癌的阳性率为92.6% (25/27),两者比较差异亦无统计学意义(P =0.556).FICE染色放大内镜可清晰观察乳头内毛细血管袢(IPCL)形态并进行分型,早期食管癌和高级别上皮内瘤变IPCL分型主要为Ⅳ和Ⅴ型,低级别上皮内瘤变和食管炎主要为Ⅱ和Ⅲ型,正常食管主要为Ⅰ型;而2% Lugol液染色放大内镜尚不能清晰观察IPCL分型.FICE染色内镜模式下无不良反应发生;2% Lugol液染色内镜下不良反应发生率为12.8% (33/257).结论 FICE染色放大内镜能准确判断早期食管癌病理分型,提高食管癌及癌前病变的诊断率,是Lugol液染色内镜的有效补充.%Objective To evaluate the flexible spectral imaging color enhancement (FICE) system in the diagnosis of early esophageal carcinoma and precancerous lesions.Methods A total of 257 patients with suspicious esophageal lesions were examined successively by FICE,magnifying FICE,iodine dyeing endoscopy and magnifying iodine dyeing endoscopy.Findings were compared with the pathologic diagnosis.Results The positive rates of early esophageal carcinoma by FICE (92.6%,25/27) and iodine dyeing endoscopy (88.9%,24/27) were not significantly different (P =0.642),nor were those of magnifying FICE (96.3%,26/27) and magnifying iodine dyeing endoscopy (92.6%,25/27),(P =0.556).The magnifying FICE

  3. Prevalence of human papillomavirus infection, distribution of viral types and risk factors in cervical samples from human immunodeficiency virus-positive women attending three human immunodeficiency virus-acquired immune deficiency syndrome reference centres in northeastern Brazil

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    Albert Eduardo Silva Martins

    2014-09-01

    Full Text Available Human immunodeficiency virus (HIV-positive patients have a greater prevalence of coinfection with human papillomavirus (HPV is of high oncogenic risk. Indeed, the presence of the virus favours intraepithelial squamous cell lesion progression and may induce cancer. The aim of this study was to evaluate the prevalence of HPV infection, distribution of HPV types and risk factors among HIV-positive patients. Cervical samples from 450 HIV-positive patients were analysed with regard to oncotic cytology, colposcopy and HPV presence and type by means of polymerase chain reaction and sequencing. The results were analysed by comparing demographic data and data relating to HPV and HIV infection. The prevalence of HPV was 47.5%. Among the HPV-positive samples, 59% included viral types of high oncogenic risk. Multivariate analysis showed an association between HPV infection and the presence of cytological alterations (p = 0.003, age greater than or equal to 35 years (p = 0.002, number of partners greater than three (p = 0.002, CD4+ lymphocyte count < 200/mm3 (p = 0.041 and alcohol abuse (p = 0.004. Although high-risk HPV was present in the majority of the lesions studied, the low frequency of HPV 16 (3.3%, low occurrence of cervical lesions and preserved immunological state in most of the HIV-positive patients were factors that may explain the low occurrence of precancerous cervical lesions in this population.

  4. Expenditure and resource utilisation for cervical screening in Australia

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    Lew Jie-Bin

    2012-12-01

    Full Text Available Abstract Background The National Cervical Screening Program in Australia currently recommends that women aged 18–69 years are screened with conventional cytology every 2 years. Publicly funded HPV vaccination was introduced in 2007, and partly as a consequence, a renewal of the screening program that includes a review of screening recommendations has recently been announced. This study aimed to provide a baseline for such a review by quantifying screening program resource utilisation and costs in 2010. Methods A detailed model of current cervical screening practice in Australia was constructed and we used data from the Victorian Cervical Cytology Registry to model age-specific compliance with screening and follow-up. We applied model-derived rate estimates to the 2010 Australian female population to calculate costs and numbers of colposcopies, biopsies, treatments for precancer and cervical cancers in that year, assuming that the numbers of these procedures were not yet substantially impacted by vaccination. Results The total cost of the screening program in 2010 (excluding administrative program overheads was estimated to be A$194.8M. We estimated that a total of 1.7 million primary screening smears costing $96.7M were conducted, a further 188,900 smears costing $10.9M were conducted to follow-up low grade abnormalities, 70,900 colposcopy and 34,100 histological evaluations together costing $21.2M were conducted, and about 18,900 treatments for precancerous lesions were performed (including retreatments, associated with a cost of $45.5M for treatment and post-treatment follow-up. We also estimated that $20.5M was spent on work-up and treatment for approximately 761 women diagnosed with invasive cervical cancer. Overall, an estimated $23 was spent in 2010 for each adult woman in Australia on cervical screening program-related activities. Conclusions Approximately half of the total cost of the screening program is spent on delivery of primary

  5. Three-year clinical performance of a HEMA-free one-step self-etch adhesive in non-carious cervical lesions.

    Science.gov (United States)

    Van Landuyt, Kirsten L; Peumans, Marleen; De Munck, Jan; Cardoso, Marcio V; Ermis, Banu; Van Meerbeek, Bart

    2011-12-01

    Despite the fact that one-step adhesives are currently used routinely in clinical practice, long-term studies on their clinical performance are scarce. The objective of this randomized controlled clinical trial was to test the hypothesis that a 2-hydroxyethyl methacrylate (HEMA)-free one-step self-etch adhesive performs worse than a conventional multistep etch-and-rinse adhesive. Two-hundred and seventy-six non-carious cervical lesions in 52 patients were restored with a micro-hybrid composite (Gradia Direct; GC). These restorations were bonded in random order either with the HEMA-free one-step adhesive G-Bond (GC) or with the 'gold-standard' (control) three-step adhesive Optibond FL (Kerr). The restorations were evaluated after 6, 12, 24, and 36 months of clinical service regarding retention, marginal adaptation, microleakage, caries occurrence, and sensitivity. After a medium-long period of 3 yr, similar success in clinical performance was observed for the simplified all-in-one adhesive and the conventional three-step adhesive. However, the one-step adhesive exhibited significantly more incisal marginal defects and discolorations. Whereas marginal degradation appeared to arrest for the multistep etch-and-rinse adhesive after 12 months, the enamel margins of the restorations bonded with the one-step self-etch adhesive continued to deteriorate. These incisal marginal defects were, however, small and could easily be removed by polishing. For both adhesives, large and sclerosed lesions appeared to be at higher risk of retention loss. PMID:22112039

  6. Application of liquid -based cytology with colposcopy in diagnosis of cervical lesions%液基细胞学结合阴道镜检查在诊断宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    周晓花

    2011-01-01

    Objective To explore and evaluate the liquid -based cytology(TCT) and colposcopy for cervical lesions.Methods The diagnostic criteria for pathological results in our hospital colposcopy, biopsy and the cervical lesions TCT test results were analyzed.Results TCT compared with the biopsy tissue, benign coincidence rate was 98.4% , LSIL, HSIL, cancer diagnosis rate was 85.4% ,97% and 100%; Colposcopy compared with histologica findings, CIN or more colposcopy cervical lesions was 97.8% compliance.Conclusions TCT consistent with colposcopy was no significant difference( P > 0.05 ), application of TCT assisted colposcopy can improve cervical lesions and cancer detection rate.%目的 探讨和评价液基细胞学(TCT)结合阴道镜对宫颈病变的诊断价值.方法 以病理学结果为诊断标准,对在永城市人民医院进行阴道镜、活检及TCT的宫颈病变患者的检查结果进行分析.结果 TCT与活检病理组织学相比,良性符合率为98.4%,LSIL、HSIL、癌的诊断符合率分别为85.4%、97%和100%;阴道镜检查结果与组织学相比,CIN以上的宫颈病变阴道镜符合率为97.8%.结论 TCT与阴道镜检查符合率无统计学意义(P>0.05),应用TCT辅助阴道镜检查可提高宫颈癌前病变和癌变的检出率.

  7. One year comparative clinical evaluation of EQUIA with resin-modified glass ionomer and a nanohybrid composite in noncarious cervical lesions

    Directory of Open Access Journals (Sweden)

    Deepa Sunil Vaid

    2015-01-01

    Full Text Available Aims: Comparative evaluation of EQUIA with a resin-modified glass ionomer cement (RMGIC; GC Gold Label glass ionomer light cured universal restorative cement and a nanohybrid composite (Tetric N-Ceram in noncarious cervical lesions (NCCLs. Background: To establish the most suitable material for the restoration of NCCLs. Settings and Design: In vivo study. Materials and Methods: Eighty-seven NCCLs were randomly restored with EQUIA, a RMGIC, and a nanohybrid composite. Clinical evaluation of the restorations was done following the Unites States Public Health criteria by a single-blinded investigator. Data were formulated, and statistical analysis was done by Chi-square test. Statistical Analysis Used: Chi-square test. Results: No significant difference was found between EQUIA, RMGIC, and nanohybrid composite at 1-month, at 6 months, and at 1-year (P > 0.05. Conclusions: EQUIA, resin-modified glass ionomer, and nanohybrid composite performed equally at 1-month, 6 months, and 1-year follow-up periods.

  8. 多种HPV亚型检测在宫颈病变诊治中的意义%Significances of detections of multiple human papillomavirus subtypes in diagnosis and treatment of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    李娟; 尹格平; 陈铭; 朱彤宇; 崔晓宁; 提松梅

    2012-01-01

    目的:通过研究不同HPV亚型与宫颈病变之间的关系,为宫颈病变的筛查和防治提供理论基础.方法:采用导流杂交基因芯片技术对1 430例宫颈病变患者进行21种HPV亚型检测,包括16种高危亚型(HR - HPV)和5种低危亚型(LR- HPV),对阳性患者行宫颈多点活体组织检查(活检),以组织病理学诊断作为宫颈病变确诊的金标准.结果:①1 430例宫颈病变患者中检测到HPV阳性患者210例,阳性率为14.68%,其中,感染HR - HPV 174例(82.86%),感染LR - HPV 36例(17.14%);居前6位的感染亚型是:HPV 16 (43.81%)、52 (15.71%)、11 (10.00%)、58 (8.57%)、31 (7.62%)、33(7.14%).其中单一感染176例(83.81%),多重感染34例(16.19%).②HR - HPV感染致CIN和宫颈癌的发病率明显高于LR - HPV; HR - HPV的感染率随宫颈病变程度的加重而逐渐增高,其中,宫颈癌组HR - HPV感染率与对照组比较差异有统计学意义(P<0.05).③宫颈癌组HPV多重感染率与对照组比较差异有统计学意义(P<0.05).结论:不同HPV亚型感染可导致不同的宫颈病变,并且与宫颈病变的不同程度密切相关.HPV多重感染与宫颈癌的发病密切相关.多种HPV亚型检测在宫颈病变筛查和防治过程中具有重要意义.%Objective: To provide theoretical basis for screening, prevention, and treatment of cervical lesions by investigating the relationship between different human papillomavirus ( HPV) subtypes and cervical lesions. Methods; Flow - through hybridization gene chip technique was used to detect 21 kinds of HPV subtypes among 1 430 patients with cervical lesions, 16 kinds of high risk HPV subtypes and 5 kinds of low risk HPV subtypes were included, the positive patients received multiple punch cervical biopsy, histopathological diagnosis was used as gold standard of cervical lesions. Results; Among 1 430 patients with cervical lesions, 210 patients were found with HPV, the positive rate was 14. 68

  9. 微创手术及传统手术治疗声门型早期喉癌及癌前病变的临床效果比较%Comparing of the Clinical Effect of Minimally Invasive Operation and Traditional Operation in Treatment of Early Glottic Carcinoma and Precancerous Lesions XIAO Qi-zhen.(Department of ENT, People′s Hospital of Jianyang,Jianyang 641400,China)

    Institute of Scientific and Technical Information of China (English)

    肖其珍

    2015-01-01

    目的:比较微创低温等离子射频消融术与传统喉切开手术治疗方式在治疗声门型早期及癌前病变的治疗效果。方法将简阳市人民医院耳鼻喉科于2013年2月至2014年5月收治的早期喉癌(声门型)及癌前病变患者85例,根据随机数字表法分为低温等离子射频消融术组( M 组)43例,传统喉切开治疗手术组( T组)42例,于术后1个月对患者进行电子喉镜复检。比较两组患者短期治疗效果及复发率,视觉模拟评分法( VAS)疼痛评估,及治疗满意度。结果 M 组平均手术时间明显较T组平均手术时间短[(11.1±2.4) min 比(108.9±13.8) min,P<0.05]。两组患者治疗后1、3、5 d VAS 评分均逐渐下降,但均高于治疗前[ M 组:(3.24±1.03)分、(2.97±0.96)分、(1.03±0.38)分比(0.56±0.15)分;T 组:(8.47±1.58)分、(8.02±2.64)分、(5.85±2.02)分比(0.57±0.13)分](P<0.05),M组的VAS评分低于T组(P<0.05)。1年后T组复发5例,M组复发1例。结论与传统喉切开手术治疗方式相比,微创低温等离子射频消融术具有手术时间短、术后恢复效果好、复发率低的优点,可显著提高患者生活质量。%Objective To compare curative effect of minimally invasive radiofrequency ablation with traditional open operation in the treatment of early laryngeal glottic type cancer and precancerous lesions . Methods A total of 85 early laryngeal glottic type cancer and precancerous lesions patients in department of ENT Jianyang People′s Hospital from Feb.2013 to May 2014 were divided into minimally invasive radiofre-quency ablation group (group M,43 cases)and traditional open operation group (group T,42 cases), according to the random number table method.After one month the patients were reexamined with electronic laryngoscope.The short-term therapeutic efficacy and recurrence rate, VAS pain assessment, and the satis

  10. Cytological diagnosis in cervical cancer

    OpenAIRE

    Mariana Bratu; Florentina Pricop; Ovidiu Toma; Dragos Crauciuc; Eduard Crauciuc

    2010-01-01

    Aim. The cytological test has multiple valences, allowing the early discovery and location of feminine genital cancer. Material and methods. In the period of time between 2001 and 2009, the study made within the Obstetrics and Gynecology Department of „Sf. Apostol Andrei” Emergency Hospital in Galaţi, revealed that from 415 cases with a changed PAP smear, the cytological diagnosis showed cancerous and pre-cancerous lesions in 53 patients (12.8%). We harvested cytological smears fo...

  11. Clinical Value of Liquid-based Cytology in the Diagnosis of Cervical Lesions%液基细胞学检查在诊断宫颈病变中的临床价值

    Institute of Scientific and Technical Information of China (English)

    姜丽红; 杨滨

    2011-01-01

    [目的]探讨宫颈液基细胞学检查(TCT)对宫颈病变的诊断价值及意义.[方法]回顾性分析中国医科大学附属盛京医院门诊57 784例TCT筛查结果.细胞学诊断采用TBS(2001)分级报告系统,阳性诊断包括意义不明确的不典型鳞状细胞(ASC-US)及以上病变,其中阳性者554例行阴道镜下宫颈活组织检查,其中317例同时又进行了宫颈高危型人乳头瘤病毒DNA(HPV-DNA)检测,对三者进行了对比分析.另外对经宫颈活检证实为宫颈癌并同时有TCT检测结果的103例患者结果也进行了对比分析.[结果]①TCT检查阳性结果与宫颈活检诊断符合率:鳞状细胞癌(SCC)3例及腺癌4例均符合组织病理学诊断;高级别鳞状上皮内病变(HSIL)符合率为86.67%(91/105),低级别鳞状上皮内瘤变(LSIL)符合率为33.93% (38/112);②经宫颈活检证实为原位癌和SCC患者TCT与宫颈活检诊断的符合率为28.57%(26/91),假阴性率38.46%(35/91),经宫颈活检证实为腺癌患者TCT与宫颈活检诊断的符合率为50.00%(6/12),假阴性率41.67%(5/12);③TCT提示HPV感染与宫颈HPV-DNA检测的符合率为79.00%(173/219);④宫颈高危型HPV-DNA 阳性检出率与组织病理学分级正相关.[结论]TCT用于宫颈病变筛查,能够及时发现大量的宫颈病变,TCT结果为HSIL及宫颈癌的患者与宫颈活检诊断的符合率较高.TCT提示HPV感染有较高的准确性,但TCT对宫颈癌筛查有较高的假阴性率.对于TCT检查阴性、而临床高度可疑恶性病变、年龄较大的患者应予以重视,进一步做阴道镜检查及高危型HPV-DNA检测和宫颈活组织检查,尤其是宫颈管诊刮,以降低宫颈癌的漏诊率.高危型HPV-DNA检测可以作为TCT诊断的客观控制指标,还可作为ASC-US及LSIL患者分流无病与患病人群的检查.%[Objective] To explore the value of liquid-based cervical cytology(TCT) for the diagnosis of cervical lesions and its significance. [Methods] The TCT

  12. Clinical effectiveness of a one-step self-etch adhesive in non-carious cervical lesions at 2 years.

    Science.gov (United States)

    Ermis, R Banu; Van Landuyt, Kirsten L; Cardoso, Marcio Vivan; De Munck, Jan; Van Meerbeek, Bart; Peumans, Marleen

    2012-06-01

    A 2-year randomized, controlled prospective study evaluated the clinical effectiveness of a one-step self-etch adhesive and a "gold-standard" three-step etch-and-rinse adhesive in non-carious Class-V lesions. The null hypothesis tested was that the one-step self-etch adhesive does perform clinically equally well as the three-step etch-and-rinse adhesive. A total of 161 lesions in 26 patients were restored with Clearfil AP-X (Kuraray). The restorations were bonded either with the "all-in-one" adhesive Clearfil S3 Bond (Kuraray) or with the three-step etch-and-rinse adhesive Optibond FL (Kerr). The restorations were evaluated at baseline and after 6 months, 1 and 2 years, regarding their retention, marginal adapation, marginal discoloration, caries occurrence, preservation of tooth vitality and post-operative sensivity. Retention loss, severe marginal defects and/or discoloration that needed intervention (repair or replacement) and the occurrence of caries were considered as clinical failures. The recall rate at 2 years was 93.8%. Only one Clearfil S3 Bond restoration was lost at the 2-year recall. All other restorations were clinically acceptable. The number of restorations with defect-free margins decreased severely during the 2-year study period (to 6.7% and 25.3% for Clearfil S3 Bond and Optibond FL, respectively). The Clearfil S3 Bond restorations presented significantly more small marginal defects at the enamel side than the Optibond FL restorations (Clearfil S3 Bond: 93.3%; Optibond FL: 73.3%; p = 0.000). Superficial marginal discoloration increased in both groups (to 53.3% and 36% for Clearfil S3 Bond and Optibond FL, respectively) and was also more pronounced in the Clearfil S3 Bond group (p = 0.007). After 2 years, the simplified one-step self-etch adhesive Clearfil S3 Bond and the three-step etch-and-rinse adhesive Optibond FL were clinically equally successful, even though both adhesives were characterized by progressive degradation in marginal

  13. Serial type-specific human papillomavirus (HPV) load measurement allows differentiation between regressing cervical lesions and serial virion productive transient infections

    International Nuclear Information System (INIS)

    Persistent high-risk human papillomavirus (HPV) infection is strongly associated with the development of high-grade cervical intraepithelial neoplasia (CIN) or cancer. Not all persistent infections lead to cancer. Viral load measured at a single time-point is a poor predictor of the natural history of HPV infections. However the profile of viral load evolution over time could distinguish nonprogressive from progressive (carcinogenic) infections. A retrospective natural history study was set up using a Belgian laboratory database including more than 800,000 liquid cytology specimens. All samples were submitted to qPCR identifying E6/E7 genes of 18 HPV types. Viral load changes over time were assessed by the linear regression slope. Database search identified 261 untreated women with persistent type-specific HPV DNA detected (270 infections) in at least three of the last smears for a average period of 3.2 years. Using the coefficient of determination (R²) infections could be subdivided in a latency group (n = 143; R² < 0.85) and a regressing group (n = 127; R² ≥ 0.85). In (≥3) serial viral load measurements, serial transient infections with latency is characterized by a nonlinear limited difference in decrease or increase of type-specific viral load (R² < 0.85 and slopes between 2 measurements 0.0010 and −0.0010 HPV copies/cell per day) over a longer period of time (1553 days), whereas regression of a clonal cell population is characterized by a linear (R² ≥ 0.85) decrease (−0.0033 HPV copies/cell per day) over a shorter period of time (708 days; P < 0.001). Using serial HPV type-specific viral load measurements we could for the first time identify regressing CIN2 and CIN3 lesions. Evolution of the viral load is an objective measurable indicator of the natural history of HPV infections and could be used for future triage in HPV-based cervical screening programs

  14. 孕期雌激素水平在宫颈癌早期病变中的影响%The Influence of Estrogen Level during Pregnancy in Cervical Cancer Early Lesion

    Institute of Scientific and Technical Information of China (English)

    陆建军

    2015-01-01

    Objective:To analyze the effect of estrogen level during pregnancy in cervical cancer early lesion.Method:220 cases of postpartum outpatients with cervical liquid-based cytology technology (LCT) and 250 cases of cervical cancer screening in our hospital from March 2012 to December 2014 were randomly selected,and then analyzed the pathlogy reports.Result:The positive rate of postpartum outpatients group was 10.0%,the cervical cancer screening group was 5.2%.The difference was statistically significant(P<0.05).Conclusion:Elevated level of estrogen has promoting effect in the diagnosis of cervical cancer early lesion.%目的:分析孕期妇女雌激素变化在宫颈癌早期病变中的影响。方法:随机选择2012年3月-2014年12月笔者所在医院220例产后门诊与同期250例两癌筛查项目中宫颈癌筛查的液基细胞学检查,并分析病理报告。结果:产后门诊组阳性率为10.0%,同期宫颈癌筛查组阳性率为5.2%,差异有统计学意义(P<0.05)。结论:孕期雌激素的剧烈变化对宫颈癌早期病变诊断有促进作用。

  15. DNA甲基转移酶1与甲基-CpG-结合蛋白2异常表达在宫颈病变中的作用及相互关系%Effect of DNA methyltransferase 1 and methyl-CpG-binding protein 2 abnormal expression on cervical lesions and related interaction

    Institute of Scientific and Technical Information of China (English)

    康慧杰; 王金桃; 高晨菲; 白丽霞; 丁玲; 许娟; 吴婷婷; 白兰

    2013-01-01

    目的 探讨DNA甲基转移酶l(DNMT)和甲基-CpG-结合蛋白2(MeCP2)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者74例、低度宫颈上皮内瘤样变(CINⅠ)患者52例、高度宫颈上皮内瘤样变(CINⅡ-Ⅲ)患者60例和宫颈炎(CI)患者58例为研究对象.在收集全部对象人口学特征、人乳头瘤病毒感染、生殖因素等相关资料同时,检测DNMT1和MeCP2蛋白(Western blot法)和mRNA (real-time PCR法)的相对表达量.采用SPSS 17.0分析软件,计算相关资料的t检验、x2检验、因素与疾病之间关联强度指标(OR值及其95%CI)及其交互作用.结果 随着宫颈病变的加重,DNMT1和MeCP2蛋白表达水平逐渐增高(H=94.33,P<0.001;F=21.580,P<0.001),DNMTl和MeCP2 mRNA的表达水平亦逐渐增高(F=4.758,P=0.003; F=7.804,P<0.001).相关分析表明,DNMT1与MeCP2(r=0.287,P<0.001)和rnRNA(r=0.179,P=0.005)表达均存在正相关关系.交互作用分析显示,DNMT1与MeCP2蛋白和mRNA高表达在SCC组和CINⅡ-Ⅲ组存在正相加交互作用.结论 DNMT1及MeCP2高表达可增加宫颈癌和癌前病变发生的风险,两者在CIN Ⅱ-Ⅲ组和SCC组存在正相加交互作用.%Objective To explore the effect of DNA methyltransferase 1 (DNMT1) and methyl-CpG-binding protein 2 (MeCP2) on cervical cancer and cervix precancerous lesion.Methods 74 patients with cervix squamous cell carcinoma (SCC),52 patients with cervical intraepithelial neoplasm Ⅰ (CIN Ⅰ),60 patients with cervical intraepithelial neoplasm Ⅱ-Ⅲ(CIN Ⅱ-Ⅲ) and 58 patients with histologically diagnosed cervix inflammation (CI),were included in this study.Information as demography,reproductive history,life style,HPV infection were collected.Western Blot were used to detect the expression of DNMT1 protein and MeCP2 protein.Real-time PCR was used to detect the expression of DNMT 1 and MeCP2 mRNA.Results Levels of DNMT1 and MeCP2

  16. Emprego da submucosa de intestino delgado porcina no reparo de lesões do esôfago cervical. Estudo experimental em cães Small intestine submucosa for repair of cervical esophageal lesions in a dog model

    Directory of Open Access Journals (Sweden)

    Zacarias Alves de Souza Filho

    2003-06-01

    Full Text Available OBJETIVO: Pesquisar a eficácia da submucosa de intestino delgado (SID porcina como enxerto para reparo de lesões em esôfago cervical de cães. MÉTODOS: Foram operados 10 cães. Ressecou-se parcialmente uma porção da parede anterior do esôfago que foi substituída por um enxerto de SID. Avaliaram-se a resistência tensiométrica, o aspecto macroscópico, a regeneração tecidual e a concentração de colágeno da porção esofágica onde se implantou o enxerto. RESULTADOS: Não houve infecção, fístula ou estenose. Verificou-se ganho semelhante de resistência do esôfago operado em relação ao controle: 31.84N contra 28.60N em média (p=0,593. A macroscopia revelou cicatrização completa com pouca retração tecidual. O estudo anátomo-patológico por HE mostrou re-epitelização completa da mucosa, proliferação vascular discreta a moderada e proliferação fibroblástica intensa. Na análise do colágeno pelo Sirus-red obteve-se em média 54.04% de colágeno tipo I, 16,04% de colágeno tipo III e 71.58% de colágeno total. CONCLUSÃO: A SID mostrou ser, no cão, um enxerto eficaz no reparo de lesões maiores do esôfago, apresentando-se resistente à infecção e à rejeição. A SID deve ser, portanto, considerada opção importante no tratamento destas lesões.PURPOSE: Study of porcine small intestinal submucosa (SIS efficiency as a graft in the handling of lesions of the cervical esophagus in dogs. METHODS: Ten dogs were operated. A resection of a portion of the anterior wall of the esophagus was performed, followed by its repair with a SIS graft. The tensiometric resistance, macroscopic appearance regeneration process and collagen contents of the esophagus tissue were evaluated. RESULTS: No cases of fistula, stenosis or infection occured. Tensiometric evaluation showed a similar resistance in the operated esophagus when compared to the control (an average of 31.84N against 28.60N. Macroscopic analysis revealed complete healing

  17. Gain of human telomerase RNA gene is associated with progression of cervical intraepithelial neoplasia grade Ⅰ or Ⅱ

    Institute of Scientific and Technical Information of China (English)

    LAN Yong-lian; YU Lan; JIA Chan-wei; WU Yu-mei; WANG Shu-yu

    2012-01-01

    Background The 3q26 chromosome region,where the human telomerase RNA gene (hTERC) is located,is a biomarker for cervical cancer and precancerous lesions.The aim of this study was to confirm the value of measuring hTERC gene gain in predicting the progression of cervical intraepithelial neoplasia grade Ⅰ or Ⅱ (CIN-Ⅰ and -Ⅱ,respectively) to CIN-Ⅲ and cervical cancer.Methods Liquid-based cytological samples from 54 patients with CIN-Ⅰ or CIN-Ⅱ lesions were enrolled in this study.Follow-up was performed with colposcopy and biopsy within 24 months after the diagnosis of CIN-Ⅰ or CIN-Ⅱ.Copy numbers of the hTERC gene were measured by fluorescence in situ hybridization with a dual-color probe mix containing the hTERC gene probe (labeled red) and the control,the chromosome 3 centromere-specific probe (labeled green).Results All patients whose lesions progressed from CIN-Ⅰ or CIN-Ⅱ to CIN-Ⅲ displayed a gain of the hTERC gene,whereas patients where the hTERC gene was not amplified did not subsequently progress to CIN-Ⅲ or cervical cancer.The signal ratio pattem per cell was recorded as N∶N (green∶ red).The numbers of cells with the signal ratio pattern of 4∶4or N∶≥5 in patients whose lesions progressed to CIN-Ⅲ were significantly higher than those whose lesions did not progress.Significantly,none of the patients with a 4∶4 signal ratio pattern regressed spontaneously.Conclusions In conclusion,measurement of hTERC gene gain in CIN-Ⅰ or CIN-Ⅱ patients using liquid-based cytological samples could be a useful biomarker to predict the progression of such cervical lesions.In addition,a 4∶4 or N∶≥5 signal ratio pattern may indicate the unlikeness of spontaneous regression of CIN-Ⅰ or CIN-Ⅱ lesions.

  18. A strategic assessment of cervical cancer prevention and treatment services in 3 districts of Uttar Pradesh, India

    Directory of Open Access Journals (Sweden)

    Dzuba Ilana

    2005-12-01

    Full Text Available Abstract Background Despite being a preventable disease, cervical cancer claims the lives of almost half a million women worldwide each year. India bears one-fifth of the global burden of the disease, with approximately 130,000 new cases a year. In an effort to assess the need and potential for improving the quality of cervical cancer prevention and treatment services in Uttar Pradesh, a strategic assessment was conducted in three of the state's districts: Agra, Lucknow, and Saharanpur. Methods Using an adaptation of stage one of the World Health Organization's Strategic Approach to Improving Reproductive Health Policies and Programmes, an assessment of the quality of cervical cancer services was carried out by a multidisciplinary team of stakeholders. The assessment included a review of the available literature, observations of services, collection of hospital statistics and the conduct of qualitative research (in-depth interviews and focus group discussions to assess the perspectives of women, providers, policy makers and community members. Results There were gaps in provider knowledge and practices, potentially attributable to limited provider training and professional development opportunities. In the absence of a state policy on cervical cancer, screening of asymptomatic women was practically absent, except in the military sector. Cytology-based cancer screening tests (i.e. pap smears were often used to help diagnose women with symptoms of reproductive tract infections but not routinely screen asymptomatic women. Access to appropriate treatment of precancerous lesions was limited and often inappropriately managed by hysterectomy in many urban centers. Cancer treatment facilities were well equipped but mostly inaccessible for women in need. Finally, policy makers, community members and clients were mostly unaware about cervical cancer and its preventable nature, although with information, expressed a strong interest in having services

  19. 深入分析LEEP活检术在宫颈病变中的诊断价值%In-depth analysis of the diagnostic value of LEEP biopsy in cervical lesions

    Institute of Scientific and Technical Information of China (English)

    吴悦; 孙跃峰

    2014-01-01

    Objective to study the application value of leeP biopsy in patients with cervical lesions. Methods the data of 31 cases of patients with cervical lesions in our hospital were retrospectively analyzed. they were detected by liquid-based cytology test of multiple biopsies with colposcopy showing low diagnostic rate and also they were positive HPV test patients. then they were tested by leeP biopsy.Results The ifndings in patients with cervical lesions for LEEP biopsy showed four cases with inlfammation, 5 cases with low-grade squamous intraepithelial lesions of CNⅠ, 10 cases of cnⅡ, 8 cases of cnⅢ, 1 case with squamous cell carcinoma, three cases with adenocarcinoma. also, of 31 cases with positive HPV test, 12 cases of low-risk patients, 19 cases of high-risk patients. The high-risk rates were:inlfammation 0, 40%of low-grade squamous intraepithelial lesions of cnⅠ, 75%of cnⅡ, 87.5%of cnⅢ, 100%of squamous cell carcinoma, adenocarcinoma 0. Conclusion leeP biopsy accurately diagnoses patients with cervical lesions, which can be widely distributed in clinical applications.%目的:研究应用leeP活检术在宫颈病变患者检查中的应用价值。方法我院的31例宫颈病变患者的案例进行回顾性分析,31例宫颈病变患者均为阴道镜下多点活检比液基薄层细胞检测结果低诊且HPV检测阳性的患者,进行leeP活检。结果经leeP活检的宫颈病变患者的检查结果为:炎症4例,鳞状上皮内低度病变cnⅠ5例,cnⅡ10例, cnⅢ8例,鳞状细胞癌1例,腺癌3例;31例HPV检测阳性患者中,低危型12例患者,高危型19例患者,各项高危率分别为:炎症0,鳞状上皮内低度病变cnⅠ40%,cnⅡ75%,cnⅢ87.5%,鳞状细胞癌100%,腺癌0。

  20. Associação entre o polimorfismo no códon 72 da p53 e as lesões pré-malignas e malignas cervicais Association of codon 72 polymorphism with premalignant and malignant cervical lesions

    Directory of Open Access Journals (Sweden)

    Fernando Anschau

    2005-10-01

    Full Text Available OBJETIVOS: testar a hipótese de que o polimorfismo no códon 72 do gene TP53 é fator de risco para as lesões pré-malignas e malignas cervicais associadas ou não ao papilomavírus humano (HPV. MÉTODOS: foram incluídas amostras de cérvice uterina, para pesquisa de DNA de HPV e do polimorfismo no códon 72 da p53 com o uso da reação em cadeia da polimerase (PCR, de 155 pacientes que se submeteram à biópsia cervical. Foram formados três grupos de acordo com o diagnóstico histológico: lesão escamosa intra-epitelial de baixo grau (LSIL, lesão escamosa intra-epitelial de alto grau (HSIL e carcinoma cervical. Aquelas pacientes sem alterações displásicas, citológicas e histológicas, foram consideradas controles. Para testar a associação entre o polimorfismo no códon 72 do gene TP53 e os grupos, foi utilizado o teste de chi2. Considerou-se como significativo o intervalo de confiança no nível de 95% (alfa=0,05. RESULTADOS: quarenta pacientes tiveram o diagnóstico histológico de carcinoma cervical, 18 tinham HSIL, 24 tinham LSIL e 73 foram consideradas controles. O genótipo Arg/Arg p53 foi encontrado em 60,0% das pacientes com câncer, 50,0% dos casos com HSIL, 45,8% dos casos com LSIL e em 45,2% dos controles. Não houve diferença significativa entre as proporções de cada genótipo da p53 nos diferentes grupos independente da presença do HPV (chi2: 3,7; p=0,716. CONCLUSÕES: nossos dados não suportam a hipótese de que o polimorfismo no códon 72 do gene TP53 é importante no desenvolvimento de lesões cervicais pré-malignas e malignas associadas ou não ao HPV.PURPOSE: to test the hypothesis that gene TP53 codon 72 polymorphism is a risk factor for premalignant and malignant cervical lesions associated or not with human papillomavirus (HPV. METHODS: uterine cervical samples were collected for HPV DNA and TP53 codon 72 polymorphism tests from 155 patients who underwent cervical biopsy. Three groups were formed according

  1. 电子阴道镜下定位活检诊断宫颈病变的临床意义%Clinical significance of diagnosis of cervical lesions under colposcopy biopsy

    Institute of Scientific and Technical Information of China (English)

    张敖

    2011-01-01

    目的 探讨电子阴道镜下定位活检诊断宫颈病变的临床意义.方法 扬州市妇幼保健院2006年12月至2008年11月经液基细胞学诊断为非典型鳞状细胞(atypicalsquamous cells,ASC)及其以上患者229例,应用电子阴道镜定位活检,评价电子阴道镜下诊断宫颈病变的价值.结果 本组经TCT筛查的细胞学阳性患者229例,其中ASCUS69例,LSIL124例,HSIL31例,SCC5例;经阴道镜下定位活检结果为宫颈炎70例;CIN 153例.其中CIN I 101例,CINII39例,CINIII 13例;浸润癌6例.结论 阴道镜下对子宫颈进行定位活检可提高CINII和CINⅢ及早期子宫颈癌的准确性和检出率,而对于子宫颈癌前期病变,尤其是早期子宫颈癌的诊断有重要的临床价值.%Objective To investigate the electronic colposcopic biopsy diagnosis of cervical lesions of clinical significance. Methods The hospital in December 2006 - November 2008 for the liquid - based cytology atypical squamous cells (atypicalsquamous cells, ASC) and above were 229 cases , the application of colposcopy biopsy, evaluation of electronic colposcopy and the value of the diagnosis of cervical lesions. Results The cytological screening by the TCT - positive patients 229 cases, of which ASCIIS 69 case, LSIL 124 cases, HSIL31 cases, SCC5 cases; by colposcopic biopsy results cervicitis 70 cases; CIN 153 cases, including CIN I 101 cases, CINII39 cases, CINIII 13 cases; invasive carcinoma in 6 cases. Conclusions Colposcopic biopsy of the cervix can be improved CINII and CIN M and early cervical cancer detection rate and accuracy, and for cervical cancer precursor lesions , especially in the early diagnosis of cervical cancer has important clinical value.

  2. AgNOR polymorphism association with squamous intraepithelial lesions and invasive carcinoma with HPV infection Asociación de los polimorfismos AgNORs con lesiones intraepiteliales escamosas, carcinoma cervical e infección por VPH

    Directory of Open Access Journals (Sweden)

    Luz del Carmen Alarcón-Romero

    2009-04-01

    Full Text Available OBJECTIVE: Evaluate the relationships between AgNORs polymorphisms and squamous intraepithelial lesions (SIL and squamous cell carcinoma (SCC with HPV infection. MATERIALS AND METHODS: A study was carried out on sixty women from the state of Guerrero, Mexico. HPV detection was performed by PCR. AgNORs were identified by argentic impregnation. One hundred cells per slide were counted and classified according to the polymorphism of AgNORs dots; typical (spherical and atypical (large, kidney-shaped and clustered. RESULTS: A total of 100% of the cases were positive for HPV infection. Nine different high-risk HPV genotypes were found, type16 was the most common (48.6%. The AgNORs showed a significant decrease in spherical shape according to neoplastic development. The three atypical shapes showed a significant increase in SIL and SCC (p-trendOBJETIVO: Evaluar la relación entre los polimorfismos de AgNORs con las lesiones intraepiteliales escamosas (LIE y carcinoma de células escamosas (CCE. MATERIAL Y MÉTODOS: Se estudiaron sesenta mujeres del estado de Guerrero, México. La detección del VPH fue por PCR y los AgNORs por impregnación argéntica; se contaron 100 células y se clasificaron por tipo de polimorfismo de AgNORs: típico (esférico y atípicos (largo, forma de riñón o de racimo. RESULTADOS: El 100% de los casos presentaron infección por VPH, se encontraron nueve genotipos diferentes de VPH de alto riesgo, el 16 fue el más común (48.6%. La forma esférica de los polimorfismos de AgNORs mostró una disminución con el desarrollo neoplásico y las atípicas incrementaron progresivamente con SIL y SCC (p-tendencia<0.001. CONCLUSIONES: Los polimorfismos AgNORs se incrementan progresivamente con el grado de lesión histológica, y pueden ser útiles en el pronóstico de progresión del carcinoma cervical.

  3. Study of the relevance of HPV 16 physical states and the degree of Cervical Lesions%人乳头瘤病毒HPV16整合与宫颈病变程度的研究

    Institute of Scientific and Technical Information of China (English)

    朱义保; 谢彤; 杨亮亮; 万磊

    2015-01-01

    目的:研究HPV 16整合与宫颈病变程度的关系,探讨反应宫颈病变的指标。方法收集感染HPV16的标本,慢性宫颈炎/CINⅠ25例(慢性宫颈炎21例,CINⅠ4例),CINⅡ22例,CIN Ⅲ23例,宫颈癌28例,对不同HPV16质粒浓度的E2和E7基因进行定量PCR分析,制作标准曲线,得出游离型和混合型HPV16 E2/E7的临界值,并对样本进行扩增,根据临界值,分析HPV16整合在不同宫颈病变的分布情况。结果标准曲线制作良好,相关系数均在0.97以上;不同HPV16质粒浓度的E2/E7比值显示,0.77为游离型和混合型HPV16的临界值;根据该临界值,发现慢性宫颈炎/CINⅠ、CINⅡ、CINⅢ、宫颈癌的HPV16的整合率分别为4.00%、9.09%、13.04%、10.71%,统计显示HPV16整合率在不同宫颈病变中没有统计学差异(P>0.05),因混合型HPV16也存在病毒整合,把整合型和混合型HPV16合并,得出HPV16总整合率在慢性宫颈炎/CINⅠ、CINⅡ、CINⅢ、宫颈癌分别为48.00%、59.09%、65.22%、75.00%,分析显示HPV16总整合率在各宫颈病变程度间亦不显著(P>0.05),HPV16总整合率在宫颈病变的分布亦无差异。结论 HPV16整合与宫颈病变程度的关系不明显,病毒整合是宫颈病变的早期事件,不能反应宫颈病变程度的变化。%Objective To study the connection of HPV 16 integration and the degree of Cervical Lesions, and investigate the in-dex of Cervical Lesions. Methods HPV16 infected samples were collected, including 21 chronic cervicitis, 4 CINⅠ, 22 CINⅡ, 23 CINⅢand 28 cervical carcinoma. Standard curves were made by amplifing E2 and E7 gene of HPV16 plasmid with different con-certration by Quantitative PCR, and the cut-off value was obtained. Then samples were detected and the distribution of HPV 16 in-tegration was analyzed in different Cervical Lesions according the cut-off value. Results The standard curves were well made, corre-lation coefficients

  4. The character and significance of the subtypes of human papillomaviruses in the cervical lesions%宫颈病变中HPV亚型感染特点及意义

    Institute of Scientific and Technical Information of China (English)

    陶琨; 杨静; 杨华; 郭振华; 陈向宇; 孟晓彦; 王春蕾; 唐龙英; 左绪磊

    2011-01-01

    Objective: To investigate the character and significance of the subtypes of human papillomaviruses in the cervical lesions. Methods: The genotypes of the HPVs in the samples of 277 cases were collected and measured by colposcopic biopsy, the HPVs in the cervical cells were analyzed and quantitatively measured by using the Hybrid Capture Ⅱ. Results: The single infection of the HPV in creased as the cervical lesions became more seriously, but the multi - infections decreased. The difference between CIN Ⅰ and CIN Ⅲ and squamous cell carcinoma were statistically significant ( P <0.05 ) . The most common subtypes of the HPVs were type 16, type 58, type 33,type 52, type 31 and type 18. Both the classification of the HPVs and the detective of the Hybrid Capture Ⅱ demonstrated that the infection rate of the HPVs increased as the cervical lesions became seriously, but the difference between the two detective methods was not statistically significant (P > 0.05) . Conclusion: The detectives of the HPV subtypes can be used in scanning the cervical lesions of the high risk population for the early diagnosis and management. The understanding of the distribution of the subtypes of the HPVs will aid the research and development of the vaccines of the human papilloma viruses.%目的:探讨宫颈病变中HPV亚型感染特点及意义.方法:收集277例阴道镜活检病例行HPV基因型测定;相应的宫颈细胞行HPV杂交捕获定量(HCⅡ)检测.结果:随着宫颈病变程度加重,HPV一重感染逐步上升,多重感染逐步下降,CIN Ⅰ组与CINⅢ组、鳞癌组比较差异有统计学意义(P<0.05);HPV亚型感染频度前六位依次为16、58、33、52、31、18;HPV分型与HCⅡ均显示随着宫颈病变程度的加重,HPV感染率上升,但两种方法检测结果差异无统计学意义(P>0.05).结论:HPV亚型检测可以用于高危人群的宫颈病变筛查,可达到早发现、早治疗的目的,并且明确HPV亚型的分布特点,有助于HPV疫苗的开发应用.

  5. 阴道镜检查及活检诊断宫颈病变240例分析%Analysis of colposcopy and biopsy in the diagnosis of cervical lesions in 240 cases

    Institute of Scientific and Technical Information of China (English)

    古丽尼沙·特海

    2015-01-01

    Objective:To explore the diagnosis value of colposcopy and biopsy in the diagnosis of cervical lesions.Methods:240 patients with cervical lesions were selected.They were given colposcopy and biopsy.Results:The colposcopy and vaginal biopsy were compared with pathological diagnosis,and the diagnose accordance rate of colposcopy was higher than that of vaginal biopsy. Conclusion:Colposcopy is better than vaginal biopsy,so colposcopy can be used as an important method of cervical disease screening.%目的:探讨阴道镜检查及活检诊断宫颈病变的诊断价值。方法:收治宫颈病变患者240例,均采用阴道镜检查及活检。结果:阴道镜检查、阴道活检与病理诊断情况比较,阴道镜检查的诊断符合率高于阴道活检。结论:阴道镜检查优于阴道活检,阴道镜检查可以作为筛查宫颈疾病的重要方法。

  6. Human papilloma virus infection in patients with cervical lesions and distribution of its subtypes%宫颈病变患者人乳头状瘤病毒感染状况及其亚型分布的研究

    Institute of Scientific and Technical Information of China (English)

    方美玉; 屠巧凤; 沈宝珠

    2012-01-01

    OBJECTIVE To investigate human papilloma virus (HPV) infections and the distribution of its subtypes in patients with cervical lesions and put forward counter measures for prevention and treatment of the disease. METHODS Cervical exfoliated cells collected from a total of 600 patients with cervical lesions were detected with Flow-through hybridization and gene chip technology, and followed by a complete evaluation of the HPV infection in related to different subtypes of cervical lesions. RESULTS The total HPV infection rate reached 78. 0%. The high-risk and low-risk HPV infection rates of patients with cervicitis/ condyloma acuminatum were 73. 1% and 18. 1%, respectively. The difference was statistically significant (P<0. 05). The high-risk and low-risk HPV infection rates of patients with cervical intraepithelial neoplasia (CIN) were 82. 6% and 7. 9%, respectively, the difference was statistically significant (P<0. 01). The high-risk and low-risk HPV infection rates of patients with cervical cancer were 96. 0% and 0, respectively, the difference was statistically significant (P<0. 01). The high-risk HPV infection rate increased with the degree of exacerbating of cervical lesions. The rate of single-type HPV infection was significantly higher than that of multi-type HPV infection in all kinds of cervical lesions (P<0. 01), while the rate of multi-type HPV infection increased with the increasing severity of cervical lesions. Different subtypes of HPV distributed differently in cervix lesions. CONCLUSION Detection of HPV subtypes is helpful for screening of the cervical lesions and diagnosis of the condition. For those patients infected especially with high-risk HPV, it is critical to conduct the early diagnosis, timely treatment and close follow-up to block the persistent HPV infection and prevent cervical cancer.%目的 探讨宫颈病变患者人乳头状瘤病毒(HPV)的感染状态及其亚型分布,提出预防治疗疾病的干预措施.方法 采用导流

  7. RELATIONSHIP BETWEEN ETIOLOGY OF CONTACT VAGINAL BLEEDING AND CERVICAL LESIONS%接触性阴道出血的病因分析及与宫颈病变的关系

    Institute of Scientific and Technical Information of China (English)

    李巧云; 刘秀荣; 李桂荣; 冯玉珍

    2015-01-01

    目的:通过对接触性阴道出血的病因进行分析,研究其与宫颈病变的关系。方法选择2012年10月—2014年10月在该院接受治疗的150例妇女作为研究对象,给予常规的妇科检查与细胞学检查。结果妇科检查结果为宫颈糜烂(62.67%)所占的比例明显高于检查的其他结果如宫颈息肉(19.33%)、阴道炎(7.33%)、尖锐湿疣(3.33%)、宫颈癌(2.67%)等,宫颈涂片细胞学检查结果为巴氏Ⅱ级所占的比例(61.33%)明显高于巴氏Ⅰ级(29.33%)、Ⅲ级(5.33%)、Ⅳ级(2.67%)与Ⅴ级(1.33%),Ⅲ~Ⅴ级患者阴道镜活检结果宫颈上皮内瘤样变Ⅰ级(35.71%)明显高于宫颈原癌(14.29%)、宫颈鳞癌(7.14%)、腺癌1例(7.14%)及宫颈上皮内瘤样变Ⅱ级(21.43%)与Ⅲ级(14.29%),差异具有统计学意义(P <0.05)。结论接触性出血病因错综复杂,多数检查结果为良性,但部分为宫颈癌的早期病兆,针对接触性出血患者建议早期检查,避免宫颈病变,减少宫颈癌的发生。%Objective To study the correlation between etiology of vaginal contact bleeding and cervical le-sions by an etiological analysis.Methods A total of 150 cases of women who achieved treatment from Oc-tober 2012 to October 2014 in author's hospital were selected as the research subjects,they were given the gynecological examination and routine cytologic examination.Results In these subjects,the gynecological examination results showed that cervical erosion (62.67%)proportion was obvious higher than that of oth-er examination results such as cervical polyp (19.33%),vaginitis (7.33%),condyloma acuminatum (3.33%),cervical cancer (2.67%).Papanicolaou grade Ⅱ accounted for the proportion of (61.33%)was obviously higher than the PAP Ⅰ grade (29.33%),Ⅲ (5.33%),Ⅳ(2.67%)and Ⅴ (1.33%).Ⅲ ~ Ⅴlevel colposcopy biopsy results in patients with cervical intraepithelial neoplasia grade I (35.71%)was sig-nificantly higher than that of primary cervical cancer (14

  8. Cervical dysplasia

    Science.gov (United States)

    ... by your provider. Make sure to get the HPV vaccine when it is offered to you. This vaccine prevents many cervical cancers. ... Early diagnosis and prompt treatment cures most cases of cervical ... severe cervical dysplasia may change into cervical cancer.

  9. 宫颈脱落细胞中的DNA甲基化研究%Study of DNA Methylation in Cervical Cytology

    Institute of Scientific and Technical Information of China (English)

    仲肇基

    2012-01-01

    在宫颈病变发生、发展的过程中,DNA甲基化水平发生一系列改变.以往的研究多是基于宫颈组织的,而近来检测宫颈脱落细胞甲基化的研究越来越多.与宫颈组织相比,用宫颈脱落细胞作为研究对象进行检测安全无创,更方便临床应用,可能成为筛查、诊断宫颈病变和判断宫颈病变预后的有利工具.讨论研究较多的宫颈脱落细胞中基因的甲基化水平与宫颈病变等级的关系及其临床应用.%Aberrant promoter methylation has been detected in pathogenesis of many cancers,including cervical cancer. During cervical carcinogenesis,there is a series of alteration in the methylation status of host genes. Recently,the role of promoter hypermethylation as a diagnostic tool for cervical cancer screening has been examined in liquid-based cytology samples. Detection of DNA methylation in exfoliated cervical cytology samples is more convenient and minimally invasive than in biopsy tissues. In those samples .researchers are searching for DNA methylation markers selectively indicating high-grade precancerous lesions, in order to increase the accuracy of cervical cytological diagnosis. The methylation markers might be used as a potential biomarker for cancer screening,diagnosis and predicting clinical outcomes. Here,we discuss methylation status of host genes in foliated cervical cytology samples during cervical carcinogenesis and its clinical impact.

  10. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women

    Science.gov (United States)

    Mandal, Paramita; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India. PMID:27198009

  11. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women

    OpenAIRE

    Mandal, Paramita; Bhattacharjee, Bornali; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan; Sengupta, Sharmila

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India.

  12. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women.

    Science.gov (United States)

    Mandal, Paramita; Bhattacharjee, Bornali; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan; Sengupta, Sharmila

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India. PMID:27198009

  13. Expression of miR-106 a in fresh and paraffin-embedded tissues and in precancerous lesions of gastric cancer%胃癌新鲜和石蜡组织及癌前病变组织中miR-106a的表达

    Institute of Scientific and Technical Information of China (English)

    朱萌; 张宁

    2015-01-01

    Purpose To investigate the comparison of miR-106a in fresh and paraffin-embedded tissues and its expression in precan-cerous 1esions of gastric cancer. Methods Human gastric cancer tissues inc1uding 30 coup1es of fresh and 40 coup1es of paraffin-em-bedded samp1es were co11ected,quantitative rea1-time PCR was used to detect the expression of miR-106a in these two samp1es. Anoth-er paraffin-embedded samp1es inc1uding 20 cases of precancerous 1esions and 40 cases of gastric adenocarcinoma were a1so co11ected,in situ hybridization was used to assess the expression of miR-106a in these stages. Results The re1ative expression of miR-106a in fresh tissues was 3. 25 ± 1. 99,in paraffin-embedded tissues was 3. 18 ± 2. 14,indicating that the expression of miR-106a in these two sam-p1es has no statistica1 difference(P>0. 05),corre1ation ana1ysis showed that there was a significant corre1ation of miR-106a expression in these two samp1es(rs =0. 998,P0.05),两组样本miR-106a的表达具有显著相关性(rs =0.998,P<0.001)。在胃癌前病变阶段可以检测到miR-106a的表达,阳性率为70%,阳性颗粒位于不典型增生的上皮细胞内,呈深蓝色细颗粒状物;胃癌组织中miR-106a的阳性率为87.5%,阳性表达范围和强度较癌前病变明显扩大和增强。结论胃癌新鲜组织和石蜡组织中miR-106a的表达呈显著正相关,应用石蜡组织检测miR-106a在胃癌前病变中出现的早期表达变化,可以为胃癌的早期诊断提供有价值的信息。

  14. The prevalence and genotype of human papillomavirus on cervical samples from an Irish female population with external genital warts.

    LENUS (Irish Health Repository)

    Cremin, Suzanne M

    2012-07-01

    The aim of this study was to determine the cervical genotype profile of females who presented to an STI Clinic with external genital warts (EGW); and to determine the potential vaccine coverage prior to the uptake of the HPV vaccines. Sixty-one cervical scrapings were taken from females aged 18-35 y who had external genital warts or a history of external genital warts. The resulting 50 samples that were positive for HPV-DNA were subjected to genotype identification. Forty-six of these samples had detectable genotypes by LIPA analysis and most (78%, 36\\/46) had multiple low risk (LR) and high risk (HR) genotypes on the cervix. Twenty-five of these samples (54%) had more than 1 HR genotype. Of the 36 patients who had any HR genotypes, 18 (50%) were identified to have the most oncogenic HPV genotypes, namely 16 and 18. Three of these samples had both 16 and 18 on the cervix. The presence of multiple HR genotypes on the majority of cervical samples from a self-referred population of females with EGW is presented. This study is of importance since persistent HR-HPV is the necessary risk factor in the development of precancerous and cancerous lesions of the cervix. Gardisil, the quadrivalent HPV vaccine would have been useful in the prevention of 28% (13\\/46) of these infections.

  15. Nuevos paradigmas y desafíos en la prevención y control del cáncer de cuello uterino en América Latina New paradigms and challenges in cervical cancer prevention and control in Latin America

    Directory of Open Access Journals (Sweden)

    Maribel Almonte

    2010-12-01

    Full Text Available El cáncer de cuello uterino sigue siendo un problema de salud pública en Latinoamérica. El uso de la citología para la detección de lesiones pre-cancerosas no ha tenido mayor impacto en las tasas de incidencia y mortalidad, que aún se mantienen altas en la región. La disponibilidad de nuevas técnicas de tamizaje para la detección de lesiones pre-cancerosas y de vacunas altamente eficaces que previenen casi todas las lesiones relacionadas con VPH-16 y VPH-18 en mujeres no expuestas previamente al virus representan una gran oportunidad para la prevención del cáncer de cuello uterino en la región. En este manuscrito resumimos la evidencia científica y la experiencia de la región en i el uso de pruebas de VPH y de la inspección visual después del ácido acético (IVAA en tamizaje primario, y ii la implementación de programas de vacunación en adolescentes. Finalmente enumeramos una serie de recomendaciones adecuadas para distintos escenarios. La factibilidad de implementar un programa nacional de prevención de cáncer de cuello uterino exitoso y sostenible en países latinoamericanos dependerá de las prioridades de salud, la infraestructura y personal de salud disponible, determinadas luego de un riguroso análisis situacional local.Cervical cancer continues to be a significant health problem in Latin America. The use of conventional cytology to detect precancerous cervical lesions has had almost no major impact on reducing cervical cancer incidence and mortality rates, which are still high in the region. The availability of new screening tools to detect precancerous lesions provide great opportunities for cervical cancer prevention in the region, as do highly efficacious HPV vaccines able to prevent nearly all lesions associated with HPV-16 and -18 when applied before viral exposure. This paper summarizes the scientific evidence and regional experiences related to: i the use of HPV testing and visual inspection after the

  16. The expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before%P16INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达

    Institute of Scientific and Technical Information of China (English)

    王志新; 肖明明

    2011-01-01

    目的:探讨P16 INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达情况.方法:采用免疫组织化学方法检测P16 INK4A与MIB-1在63例宫颈组织及38例宫颈涂片中的表达.结果:P16在正常宫颈上皮中均无表达.随着宫颈病变级别的增高,其阳性表达率逐渐增大,其中,宫颈癌中P16的表达增强明显.MIB-1随着宫颈病变级别的增高,表达增强,其中,宫颈癌中MIB-1的表达增强明显.在宫颈病变组织中,随着P16阳性表达率的增大,MIB-1表达也相应增高,具有相关性.结论:宫颈上皮内病变中P16及MIB-1过表达,提示两者可以作为宫颈病变的诊断标志物.在宫颈病变及宫颈癌的确切诊断中,P16及MIB-1可以作为诊断依据之一.%Objective: To evaluate the expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before.Methods: Immunohistochemistry methods were used to detect the expression of P16 INK4A and MIB-1 in 63 cases of cervical tissue and 38 cases of cerical smear.Results: No positive staining of P16 was observed in the normal cervical epithelium.With increasing severity of cervical intraepithelial neoplasias (CIN), the P16 expression increased progressively, significant up-regulation of P16 was observed in carcinoma cervix.MIB-1 was observed with increasing severity of CIN, and significant overexpression of MIB-1 was observed in carcinoma cervix.The expression of MIB-1 was increased with consistently increasing of P16, there was a correlation between P16 and MIB-1 in cervical lesions organization.Conclusion: The overexpression of P16 and MIB-1 demonstrates that they can be used as a diagnostic marker for cervical lesions.Therefore, in the exact diagnosis of cervical lesions and cervical cancer, P16 and MIB-1 markers can be used as one of the diagnostic bases.

  17. Prevalence and Distribution of High-Risk Genotypes of HPV in Women with Severe Cervical Lesions in Madrid, Spain: Importance of Detecting Genotype 16 and Other High-Risk Genotypes.

    Science.gov (United States)

    Mateos Lindemann, Maria Luisa; Sánchez Calvo, Juan Manuel; Chacón de Antonio, Jesús; Sanz, Itziar; Diaz, Esperanza; Rubio, Maria Dolores; de la Morena, Maria Luisa

    2011-01-01

    Background. Persistent infection with high-risk human papillomavirus (HR-HPV) has been demonstrated to be the necessary causal factor for developing cervical cancer. To know the most prevalent HR-HPV in different geographical areas is important to design diagnostic tests and implementation of vaccines. Objectives. The goal of this study is to evaluate the prevalence of HR-HPV in a total of 1001 patients, 198 with normal cytology results, 498 with low-grade squamous intraepithelial lesion (LSIL), and 205 with high-grade squamous intraepithelial lesion (HSIL) who attended our gynaecology department for opportunistic screening of HPV infection. Study design. Cervical samples were taken in a PreservCyt vial (Cytyc Corporation, Boxborough, MA). Hybrid capture assay was carried out following the manufacturer's instructions (Digene Corp., Gaithersburg, MD). All samples were further studied with polymerase chain reaction (PCR) (Linear Array HPV Genotyping Test, Roche Diagnostics, Mannheim, Germany). Results. Genotype 16 was the most prevalent HR-HPV in the three groups, 17.8% in the patients with normal cytology results, 22.3% in the LSIL group, and 60% in the HSIL group. Genotype 18 had a very low prevalence in all groups. Other HR-HPV genotypes such as genotype 31, genotype 58 and genotype 52 were found in significant numbers in HSIL patients. Discussion. Our data show that genotypes 16, 31, 58, and 52 are the most prevalent HR-HPV in cervical samples with severe intraepithelial lesion in Spain. There may be some geographical variation in prevalence of carcinogenic types, and it must be considered for designing diagnostic tests and vaccine.

  18. Value of Cervical Fluid Based Cytology and Vaginal Examination in Early Screening for Cervical Lesions%宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值探析

    Institute of Scientific and Technical Information of China (English)

    张书霞

    2015-01-01

    目的 研究和探析宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值. 方法 收集2013年1月-2014年12月到该院妇科门诊行宫颈检查的患者共1 037例,所有患者均接受宫颈液基细胞学检测,对检查结果呈阳性者进行阴道镜检查. 结果 37例(3.6%)患者宫颈液基细胞学检测呈阳性,经过阴道镜检查后,23例显示阳性,其中炎症12例(32.4%),宫颈上皮内瘤样病变Ⅰ期14例(37.8%), 宫颈上皮内瘤样病变Ⅱ~Ⅲ6例(16.2%). 阴道镜下的活检组织病理学检查结果与宫颈液基细胞学的检查结果的差异有统计学意义(P<0.05).结论 在宫颈病变早期筛查中,宫颈液基细胞学和阴道镜活组织病理检查联合使用,有助于尽早发现宫颈病变,值得推广应用.%Objective To study the application value of cervical fluid based cytology and vaginal examination in early screening for cervical lesions. Methods 1037 gynecological outpatients who underwent inspection of the cervix in the hospital between January 2013 and December 2014 were included. All of them received cervical fluid based cytology, and for those with positive results vaginoscopy was carried out. Results Out of 37 patients (3.6%) who showed positive result from cervical fluid based cytology, 23 showed positive result after vaginoscopy, including 12 (32.4%) cases of f inflammation and 14(37.8%) cases of stage I cervical in-traepithelial neoplasia, and 6 (16.2%) cases of stage II-III cervical intraepithelial neoplasia. Therefore, there was statistically sig-nificant difference in the outcomes between vaginoscopy and cervical fluid based cytology, P<0.05. Conclusion In the early screening of cervical lesions, the cervical fluid based cytology combined with pathological examination of biopsy under vaginoscope is beneficial to the early detection of cervical lesions, therefore it is worthy of popularization and application.

  19. STUDY OF HIGH RISK CASES FOR EARLY DETECTION OF CERVICAL CANCER BY PAP’S SMEAR AND VISUAL INSPECTION BY LUGOL’S IODINE METHOD.

    Directory of Open Access Journals (Sweden)

    Harshad Ladola

    2013-01-01

    Full Text Available INTRODUCTION:Cervical cancer is the commonest genital tract cancer among Indian women. Screening programmes have claimed to reduce incidence and mortality of cervical carcinoma significantly, for which sensitization of women is required through community based approach.OBJECTIVES: Comparison of VILI and cytology by PAP smear for detection of low grade squamous intraepithelial lesion, high grade squamous intraepithelial lesion, carcinoma of cervix and correlation of results with the reference standards(colposcopy and biopsyMATERIAL & METHODS:Study was carried out in 50 women of high risk group, aged 20 to 65 years in the year of May 2008 to May 2010 in our hospital. All women were investigated with colposcopy and biopsy were taken who had abnormal colposcopy.RESULT:Sensitivity, Specificity, Positive predictive value, Negative predictive value of PAP test was 80%, 97%, 80%, 97% respectively, compared with reference standards.Sensitivity, Specificity, Positive predictive value, Negative predictive value of VILI test was 80%, 91.11%,50%,97.6% respectively, compared with reference standards.CONCLUSION:VILI and PAP test can be used effectively for detection of precancerous lesion of cervix at hospital set up as well as community level.

  20. Avaliação da conduta conservadora na lesão intraepitelial cervical de alto grau Evaluación de la conducta conservadora en la lesión intraepitelial cervical de alto grado Evaluation of conservative management of high-grade cervical squamous intraepithelial lesion

    Directory of Open Access Journals (Sweden)

    Nelson Shozo Uchimura

    2012-06-01

    transversal y retrospectivo realizado con 509 mujeres (15-76 años atendidas en el período de 1996 a 2006, con colpocitología oncótica alterada, en un servicio público de referencia en Maringá, Sur de Brasil. Los datos fueron colectados de los prontuarios médicos y se estudiaron las variables diagnóstico definitivo, tipos de tratamiento, ocurrencia de la lesión y reincidencias y se analizaron por medio de pruebas de asociación de chi-cuadrado de Pearson y exacto de Fisher. RESULTADOS: La lesión intraepitelial cervical de alto grado ocurrió en 168 casos; de estos, 31 mujeres se sometieron a la amputación cónica, 104 a cirugías de alta frecuencia, nueve a histerectomías y 24 recibieron conducta conservadora. Entre las mujeres con lesión de alto grado y tratadas de forma conservadora, ocho (33,3% reincidieron, mientras que de las sometidas a la conducta no conservadora, diez (6,9% reincidieron, siendo esta diferencia estadísticamente significativa (p=0,0009, RP=4,8 (IC95% 2,11;10,93. En aquellas que hicieron seguimiento clínico-citológico, tres (30,0% y, entre las cauterizadas, cinco (35,7% reincidieron en el plazo de tres años, sin diferencia significativa (p=0,5611. La reincidencia por debajo y por encima de los 30 años ocurrió, respectivamente, en siete (13,8% y 11 (12,2% mujeres (p=0,9955. CONCLUSIONES: La edad de la mujer no influencia el pronóstico de la reincidencia. El tratamiento conservador debe ser indicado como conducta de excepción, dada la alta tasa de reincidencia, y el seguimiento debe ser riguroso, con acompañamiento citológico y colposcópico por inclusive tres años, período en el que ocurre la mayoría de las reincidencias.OBJECTIVE: To assess the association between conservative management of high-grade cervical squamous intraepithelial lesions and recurrence rates and age groups. METHODS: Cross-sectional, retrospective, analytical observational study of 509 women (aged 15 to 76 with abnormal Pap smears attending a public reference

  1. CD28-, CD45RAnull/dim and natural killer-like CD8+ T cells are increased in peripheral blood of women with low-grade cervical lesions

    OpenAIRE

    Pita-Lopez, Maria Luisa; Ortiz-Lazareno, Pablo Cesar; Navarro-Meza, Monica; Santoyo-Telles, Felipe; Peralta-Zaragoza, Oscar

    2014-01-01

    Background In response to antigen naive CD8+, T cells differentiate into effector cells, which express Natural killer (NK) receptors, lose CD28 expression, and die by apoptosis. However, in smaller quantities, the cells are retained for subsequent exposure to the same antigen. Knowledge is limited regarding whether the percentages of CD28-, Effector memory (EMRAnull/dim), and the CD16+/CD56 + CD8+ T cells of women with low-grade cervical lesions are altered at a systemic level. Methods We enr...

  2. Augmented serum level of major histocompatibility complex class I-related chain A (MICA) protein and reduced NKG2D expression on NK and T cells in patients with cervical cancer and precursor lesions

    International Nuclear Information System (INIS)

    Cervical cancer is the second most common cancer in women worldwide. NK and cytotoxic T cells play an important role in the elimination of virus-infected and tumor cells through NKG2D activating receptors, which can promote the lysis of target cells by binding to the major histocompatibility complex class I-related chain A (MICA) proteins. Increased serum levels of MICA have been found in patients with epithelial tumors. The aim of this study was to compare the levels of soluble MICA (sMICA) and NKG2D-expressing NK and T cells in blood samples from patients with cervical cancer or precursor lesions with those from healthy donors. Peripheral blood with or without heparin was collected to obtain mononuclear cells or sera, respectively. Serum sMICA levels were measured by ELISA and NKG2D-expressing immune cells were analyzed by flow cytometry. Also, a correlation analysis was performed to associate sMICA levels with either NKG2D expression or with the stage of the lesion. Significant amounts of sMICA were detected in sera from nearly all patients. We found a decrease in the number of NKG2D-expressing NK and T cells in both cervical cancer and lesion groups when compared to healthy donors. Pearson analysis showed a negative correlation between sMICA and NKG2D-expressing T cells; however, we did not find a significant correlation when the analysis was applied to sMICA and NKG2D expression on NK cells. Our results show for the first time that high sMICA levels are found in sera from patients with both cervical cancer and precursor lesions when compared with healthy donors. We also observed a diminution in the number of NKG2D-expressing NK and T cells in the patient samples; however, a significant negative correlation between sMICA and NKG2D expression was only seen in T cells

  3. Triage of women with minor cervical lesions: data suggesting a "test and treat" approach for HPV E6/E7 mRNA testing.

    Directory of Open Access Journals (Sweden)

    Sveinung Wergeland Sørbye

    Full Text Available BACKGROUND: Human papillomavirus (HPV testing is included in the cervical cancer screening program in the triage of women with equivocal (ASC-US or low-grade (LSIL cytological lesions. These women have an increased risk for developing high grade dysplasia and cancer (CIN2+ compared to women with normal cytology. However, in order to avoid unnecessary follow-up, as well as overtreatment, a high positive predictive value (PPV of the triage test is important. METHODOLOGY/PRINCIPAL FINDINGS: The HPV test PreTect HPV-Proofer, detecting E6/E7 mRNA from the HPV types 16, 18, 31, 33 and 45, is used as triage test together with repeat cytology. PPV data for HPV E6/E7 mRNA testing during the period from January 2006 up to June 2009 are reported. In total, 406 of 2099 women (19.3% had a positive HPV test result. Of the women with a positive test result and with a histological diagnosis (n = 347, 243 women had histological high-grade dysplasia or cancer (CIN2+, giving a PPV of 70.0% (95% confidence interval [CI], 65.2%-74.8%. For HPV 16 or HPV 33 positive women above 40 years of age, the PPV was 83.7% (95% CI, 73.3%-94.0% and 84.6% (95% CI, 65.0%-100.0% respectively. The PPV of test positive women with HSIL cytology was 94.2% (95% CI, 88.7%-99.7%. CONCLUSIONS: When the result in triage is HPV mRNA positive, our data suggest direct treatment for women above 40 years of age or for women with a concurrent cytological HSIL diagnosis, contributing to better clinical safety for these women. In addition, by decreasing the time to treatment, thereby reducing the number of recalls, the patient management algorithm will be considerably improved, in turn reducing follow-up costs as well as unnecessary psychological stress among patients.

  4. 18356例宫颈液基细胞学与活检组织病理学结果分析%Analysis the Results of 18 356 Cases of Liquid-based Cervical Cytology and the Histopathology of Colposcopic Biopsy in Cervical Screening/

    Institute of Scientific and Technical Information of China (English)

    江淑萍; 孔友明; 陈冰

    2013-01-01

      目的:探讨宫颈液基细胞学与活检组织病理学在宫颈病变筛查中的价值.方法:对18356例经宫颈液基细胞学检查发现异常者在阴道镜下活检的患者临床及病理资料进行回顾性分析.结果:LCT 显示:18356例中,ASCUS 以上的有1256例,阳性率为6.8%,其中 ASCUS 占51.8%,LSIL占29.5%,HSIL 占10.6%.宫颈活检:慢性炎症占39.7%,CIN Ⅰ占37.3%,CIN Ⅱ占13.6%,CIN Ⅲ占8.9%,宫颈癌占0.4%.结论:LCT 液基细胞学检测系统是一种有效的宫颈癌前病变筛查方法.对 LCT 阳性病例行阴道镜下活检,能大大提高宫颈癌前病变及宫颈癌早期检出率,减少漏诊的发生.%Objective:To evaluate the value of liquid-based cytology (LCT) and histopathology of colposcopic biopsy in cervical screening.Methods:The cervical LCT results of 18 356 cases were analyzed,follow up diagnoses of colposcopic biopsy were available for those cytological abnormalities,then we performed a retrospective analysis of those clinical and pathological data.Results:Among 18 356 patients receiving LCT,1256 patients were diagnosed as Above-ASCUS pathological changes,the positive detection rate was 6.8%.Among these,ASCUS,LSIL and HSIL were 51.8%,29.5% and 10.6%,respectively. Among 1256 patients receiving colposcopic biopsy,39.7% were diagnosed as chronic inflammation,and cervical intraepithelial neoplasiaⅠ(CINⅠ) were 37.3%, CIN Ⅱ were 13.6%,CIN Ⅲ were 8.9% and cervical cancer were 0.4%.Conclusion:LCT liquid-based cytology technology is an effective method in screening of cervical precancerous disease.Combining with colposcopic biopsy among patients with abnormal LCT results,can not only improve the detection rate of cervical precancerous lesions and early stage of cervical cancer,but also reduce the incidence of missed diagnosis,significantly.

  5. The value of TCT and HPV-DNA detection in screening of cervical lesions%TCT和HR-HPV DNA检测对宫颈病变筛查的价值

    Institute of Scientific and Technical Information of China (English)

    王琼; 刘勇; 徐子顺; 吴易红

    2015-01-01

    Objective:To investigate the value of thinprep cytology test (TCT) and high-risk human papilloma virus DNA (HR-HPV DNA) detection in the screening of cervical lesions so as to provide a theoretical basis for the government to formulate the vaccine prevention policy. Methods:Two hundred and forty-three women diagnosed or examined in our hospital were selected, to whom TCT and the detection of HR-HPV DNA were performed and their results were analyzed. Results:The speciifcity, positive predictive value and diagnosis rate were obviously higher by the combined detection of TCT and HR-HPV DNA than by single detection of HR-HPV DNA and their differences had statistical significance (P<0.05). Conclusion:The combined detection of TCT and HR-HPV DNA can improve the diagnosis level of cervical lesions and early cervical cancer, reduce the rate of misdiagnosis and has important signiifcance for the early screening of cervical lesions.%目的:探讨液基薄层细胞学检测(TCT)和高危型人乳头瘤状病毒DNA(HR-HPV DNA)检测对宫颈病变筛查的价值,为政府制定疫苗的预防政策提供理论依据。方法:选取在我院就诊或体检的妇女243例,均行TCT、HR-HPV DNA检测,分析其检测结果。结果:TCT+HR-HPV DNA联合检测的特异性、阳性预测值和诊断符合率均明显高于HR-HPV DNA单项检测,差异具有统计学意义(P<0.05)。结论:TCT和HR-HPV DNA联合检测可提高宫颈病变及早期宫颈癌的诊断水平,降低漏诊率,对宫颈病变的早期筛查具有重要意义。

  6. Research Advancement of the Correlation between Human Papillomavirus and Cervical Cancer%人乳头瘤病毒感染与宫颈癌关系的研究进展

    Institute of Scientific and Technical Information of China (English)

    王芳; 王玉欢

    2013-01-01

    宫颈癌是全球妇女中最为常见的恶性肿瘤,近年来其发病率呈明显的上升趋势,是威胁妇女健康、导致妇女死亡的主要恶性肿瘤之一.人乳头瘤病毒感染是宫颈癌发生的最关键致病因素,通常从HPV感染到宫颈癌的发生需经历3 ~8年的时间,因此通过有效的干预措施,包括筛查,处理HPV感染和癌前病变,可以有效地阻止病情的发展,从而预防宫颈癌的发生.%Cervical cancer is the most common malignant tumor in women worldwide, and in recent years its incidence shows significantly rising trend,which is one of the major malignant tumors threatening women's health or even resulting in death.Human papillomavirus infection is the main pathogenic factor of cervical cancer,and it often takes 3 to 8 years from the HPV infection to the occurrence of cervical cancer,therefore effective intervention measures,including screening,treatment of HPV infection and precancerous lesions,can effectively prevent the development of the disease, so as to prevent the occurrence of cervical cancer.

  7. Clinical study on influence of human papillomavirus infection on cervical lesions%人乳头状瘤病毒感染对宫颈病变影响的临床研究

    Institute of Scientific and Technical Information of China (English)

    赵颖辉; 梁贤; 李慧; 高凤萍

    2014-01-01

    目的:研究人乳头状瘤病毒单一感染与多重感染对宫颈病变的影响,以期为临床诊治宫颈病变提供参考依据。方法选取2012年1月-2013年3月入院诊治并应用导流杂交人乳头状瘤病毒分型检测技术确诊的人乳头状瘤病毒感染患者120例,回顾性分析其临床资料,分为人乳头状瘤病毒单一感染组73例与多重感染组47例,观察并比较两组感染对宫颈病变的影响,采用SPSS16.0进行统计分析。结果 HPV单一感染与多重感染高危型亚型分布均以16型为主,分别占35.62%、25.53%,各型分布差异有统计学意义( P<0.05),细胞学结果,诊断高度鳞状上皮内病变单一感染组与多重感染组分别占23.29%与8.51%,差异有统计学意义( P<0.05);病理学结果,单一感染组与多重感染组在诊断患者正常、CIN病变病理学结果差异有统计学意义( P<0.05)。结论单一高危人乳头状瘤病毒亚型对宫颈病变的严重程度有一定的诊断意义;多重人乳头状瘤病毒亚型感染多变现象为低级别宫颈病变。%OBJECTIVE To study the influence of single and multiple infections with human papillomavirus on cervi‐cal lesions ,in order to provide a reference for the clinical diagnosis of cervical lesions .METHODS Totally 120 patients who were admitted to hospital during Jan .2012 to Mar .2013 and confirmed with human papillomavirus infection by flow‐through hybridization detection of human papillomavirus genotyping were enrolled and their clini‐cal data were retrospectively analyzed .The patients were divided into the single infection group (n=73) and the multi‐infection group (n=47) .The two groups were observed and compared for influence of infections on cervical lesions .The software SPSS16 .0 was used for statistical analysis .RESULTS The HPV high‐risk subtype of type 16 was predominant ,accounting for 31 .67% and 25 .53% in

  8. 深圳社区女性宫颈病变防治认知情况调查分析%Questionnaires survey on the cognition of prevention and treatment for cervical lesions among females from Shenzhen communities

    Institute of Scientific and Technical Information of China (English)

    周艳秋; 杨将; 吴瑞芳

    2016-01-01

    higher in the females who were in the age of 31-40 years old, with more than 30 thousand income, with college education or above degree, civil servants or office clerks (P<0.05).Conclusions The females from communities have some knowledge about the relationship between cervical cancer and HPV, but lack comprehensive understanding of prevention and treatment of cervical cancer. Especially we need to enhance the diverse health knowledge spread in females who are young, with low-education background, with low income and labors or farmers, improving the awareness rate of prevention and treatment for cervical lesions.

  9. The research progress of vagina lactobacillus is associated with cervical lesions%阴道乳杆菌与宫颈病变相关的研究进展

    Institute of Scientific and Technical Information of China (English)

    辛秀团

    2015-01-01

    The cervical lesion is one of the major diseases threatening the health of women, in the context of its incidence, second only to breast cancer in female malignant tumor in the whole world, in some countries even than the breast cancer incidence of a disease. Mainly concentrated in cervical lesions in patients with age between 30 to 45 years old, and as the patient age incidence is obviously rising trend. At home and abroad at present, many scholars believe that the cervical lesions have direct links to the fall in the number of lactobacillus in the vagina, aimed at this situation, this study from the antitumor mechanism and original microbial antagonism disease-resistant two aspects of lactobacillus in the vagina and cervix lesion of correlation analysis, the peers to play the role of reference and reference.%宫颈病变是威胁妇女健康的主要疾病之一,就其发病率来看,在全球女性恶性肿瘤中仅次于乳腺癌,在部分国家中发病率甚至超过了乳腺癌。宫颈病变患者年龄主要集中在30-45岁之间,而且随着患者年龄的增长发病率呈明显上升趋势。目前,国内外诸多学者认为宫颈病变与阴道乳杆菌数量下降有着直接的联系,针对这一现状,本研究从抗肿瘤机制以及拮抗病原微生物两个方面对阴道乳杆菌与宫颈病变的相关性进行分析,希望能够对广大同行起到借鉴与参考的作用。

  10. 宫颈病变患者人乳头瘤病毒16亚型E2基因多态性检测%Detection of E2 gene polymorphism of human papillomavirus type 16 in patients with cervical lesions and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    邱峰; 袁丽娜; 黄宪章

    2012-01-01

    Objective To investigate the incidence of human papillomavirus type 16 (HPV16) infection in patients with cervical diseases and analyze E2 gene mutations and its correlation with cervical lesions. Methods Specific primers were designed according to the full-length E2 gene sequence of HPV16 from the GenBank. PCR was used to analyze the incidence of HFV16 infection in the exfoliated cell samples from 379 patients with different cervical diseases positive for high-risk HPV. The polymorphisms of E2 gene of HPV16 were analyzed by high-resolution melting. Results Of the 379 exfoliated cell samples from the cervix, 78 were positive for HPV16 infection. The positivity rates of HPV16 infection was 44,8% in patients with cervical cancer, 31.5% in patients with CIN Ⅱ-Ⅲ, 241% in patients with CIM I and 9.6% in patients with cervical inflammation. The frequencies of E2 gene 68C and 133G variations were significantly higher in patients with CIN Ⅱ-Ⅲ and those with cervical cancer than in those with CIN I and those with cervical inflammation (P<0.05). Conclusion HPV16 is an important cause of cervical cancer, and HPV16 infection rate increases with the pathological progression of the cervical lesions. The naturally occurring sequence variations of HPV16 E2 gene may cause alterations uf the carcinogenic potential of the virus,%目的 分析宫颈病变中人乳头瘤病毒(HPV) 16亚型E2基因的多态与宫颈病变的关系,了解E2基因变异情况及其与宫颈病变的相关性.方法 应用PCR和高分辨率熔解曲线方法针对379例HPV高危亚型阳性宫颈脱落细胞样本进行HPV16感染情况及HPV16亚型E2基因68位点和133位点多态分布情况进行检测.结果 379例HPV高危亚型阳性宫颈标本共检出78例HPV 16亚型附性,其在宫颈癌、CINⅡ~Ⅲ、CIN Ⅰ、炎症患者中的检岀率分别为44.8%、31.5%、24.1%和9.6%;HPV16 E2基因68C和133G的频率在中重度宫颈内瘤样病变和宫颈癌中明显高于轻度

  11. Analysis of the Relationship Between HPV Infection and Cervical Lesions and the Related Risk Factors%HPV感染与宫颈病变的关系及相关危险因素分析

    Institute of Scientific and Technical Information of China (English)

    张桢华; 孙翠梅; 刘春君

    2016-01-01

    目的:探讨HPV感染与宫颈病变关系,分析HPV感染的相关危险因素。方法选取2013年5月~2015年5月就诊于我院妇科并行阴道镜检查、TCT筛查、宫颈活组织检查的216例患者为研究对象。依据宫颈活检病理学诊断,分为研究组(患有宫颈病变,141例)和对照组(宫颈正常,75例),并对同时期就诊于我院妇科的196例签署知情同意书的患者进行临床资料查阅及问卷调查,回顾分析所有患者的临床、生化及问卷调查资料。结果正常宫颈、CINⅠ级、CINⅡ~CINⅢ级、宫颈癌患者HPV感染率分别为16.0%、67.1%、83.7%、95.5%,经统计分析显示正常宫颈组与宫颈病变组各病理级别HPV感染率差异有统计学意义(P<0.05),宫颈病变不同病理级别间HPV感染率比较差异也有统计学意义(P<0.05);对患者一般资料与HPV感染的关系进行分析,结果显示:年龄、受教育程度、首次性生活年龄、避孕方式差异均具有统计学意义(P<0.05)。结论HPV感染与宫颈病变病理级别呈正相关;年龄较大(>50岁)、受教育程度低(高中以下)、首次性生活年龄小(≤20岁)是HPV感染的危险因素,非避孕套避孕可增加HPV感染可能性;抗HPV生物蛋白敷料可用于治疗HPV感染,对于宫颈高危HPV感染及其引起的低级别宫颈病变均有明显治疗效果。%Objective To explore the relationship between HPV infection and cervical lesions, and analyze the related risk factors for HPV infection. Methods216 cases of routine vaginal examination, TCT screening, cervical biopsy were selected from May 2013 to May 2015 in our hospital, TCT screening. Based on cervical biopsy pathology, is divided into research group (suffering from cervical lesions, 141 cases) with cervical lesions, and the control group (normal cervix, 75 cases), and at the same time to see a doctor in the hospital of department of

  12. Performance of visual inspection with acetic acid and human papillomavirus testing for detection of high-grade cervical lesions in HIV positive and HIV negative Tanzanian women

    DEFF Research Database (Denmark)

    Dartell, Myassa Arkam; Rasch, Vibeke; Iftner, Thomas;

    2014-01-01

    The aim of this cross sectional study was to assess type distribution of human papillomavirus (HPV) among HIV positive and HIV negative women who underwent cervical cancer screening, and to examine the ability of visual inspection with acetic acid (VIA), the standard detection method in Tanzania...

  13. A shift to a peripheral Th2-type cytokine pattern during the carcinogenesis of cervical cancer becomes manifest in CIN III lesions

    NARCIS (Netherlands)

    A.G. Bais (Aagje); I. Beckmann (Ilse); J. Lindemans (Jan); P.C. Ewing (Patricia); C.J.L.M. Meijer (Chris); T.J.M. Helmerhorst (Theo); P.J.L.M. Snijders (Pieter)

    2005-01-01

    textabstractBACKGROUND: A shifted balance between T helper 1 (Th1)-type and Th2-type cytokines has been hypothesised in cervical dysplasia. AIMS: To evaluate possible deregulation of the cytokine network by estimating the expression of peripheral cytokines in different stages of ce

  14. Breast lesions in reduction mammaplasty specimens: a histopathological pattern in 534 patients

    OpenAIRE

    Tadler, M; G. Vlastos; Pelte, M-F; Tille, J-C; BOUCHARDY, C; Usel, M; Pittet-Cuénod, B.; Modarressi, A.

    2013-01-01

    Background: The prevalence of breast lesions (benign, precancerous and cancer lesions) in reduction mammaplasty (RM) specimens has rarely been reported in Europe and never in the Swiss population. Methods: Personal and histopathological data from 534 female patients who underwent RM were reviewed. Results: Benign and/or malignant lesions were detected in 76.2% of all patients. Benign breast lesions associated with an increased risk of developing breast cancer represented 2.8% of all lesions. ...

  15. 高危型人乳头瘤病毒阳性与宫颈病变关系的研究%The Research of High-risk Human Papillomavirus and Cervical Lesions Positive Relationship

    Institute of Scientific and Technical Information of China (English)

    王西兵

    2014-01-01

    Objective In recent years female cervical lesions continued to rise on, a serious threat to the women's physical and mental health, clinical began inducing factors of cervical lesions and treatment. Analysis of the relationship between positive for high-risk human papillomavirus and cervical lesions, and provide reliable suggestions for clinical treatment, the accumulation of clinical experience in the treatment of more. Methods The study selection for the 2010 June-2013 year in June, our hospital included female patient data, through the clinical comprehensive diagnosis of cervical lesions, the implementation of the necessary checks on high-risk human papillomavirus positive, to determine the specific situation of the cervical lesions. In addition to pathological examination, but also the implementation of a comprehensive examination of cervical cancer patients, mainly state the clinical disease, to detect the high-risk human papillomavirus types as the basis of practical treatment options. Results ①the case for relates to the number of 6096 cases, high-risk human papillomavirus positive were 1351 cases, the positive rate of the statistical results of high-risk human papillomavirus 22.3%;look from the age, the positive rate from high to low according to the arrangement of 25-29 years, 55-59 years, 40-45 years old, the positive rate were 29.9%, 26%, 15.3%;the positive rate of comprehensive comparison of several age groups, the results have significant difference(P<0.01).②according to the statistical results, the high-risk human papillomavirus positive cases, choose the colposcopic biopsy of 1187 cases, the final results showed 54 cases of normal cervical organizers, 833 cases of chronic cervicitis, 297 cases of cervical intraepithelial neoplasia. Take the same colposcopy examination, according to the diagnostic criteria of Chinese Medical Association Rules, the passing rate of 94.7%;in accordance with the pathology compared with other detection results theory

  16. Molecular epidemiology of human papillomavirus infections among cervical lesions women in Quzhou area%衢州地区宫颈病变女性人乳头状瘤病毒感染分子流行病学研究

    Institute of Scientific and Technical Information of China (English)

    余晓; 夏利花; 徐小敏; 周燕

    2013-01-01

    OBJECTIVE To study the molecular epidemiological characteristics of 21 genotypes of human papillomavirus (HPV) among 1280 women with cervical lesions in Quzhou area. METHODS The 21 HPV types were determined among 1280 women who suffered from cervical lesions in Quzhou area. The molecular epidemiological characteristics of 21 HPV genotypes were compared. RESULTS Of 1280 cases with cervical lesions, HPV infections occurred in 708 cases with the infection rate of 55. 31% , the patients with high-risk HPV infections accounted for 38. 83% , and the patients with low-risk HPV infections accounted for 16. 48%. HPV16, 58 and 52 were the most common high-risk genotypes, and HPV6 and 11 were the most common low-risk genotypes. The total HPV infection rate and the high-risk infection rate of the patients aged between 31 and 40 years were the highest, which were 67. 39% and 50. 58% , followed by the patients aged between 21 and 30 years, which were 61. 75% and 45. 78% respectively, the differences in the proportion of HPV infections and high-risk HPV infections between the various age groups of patients were statistically significant. CONCLUSION This study has offered the molecular epidemiological characteristics of 21 genotypes of HPV among 1280 cervical lesions women, providing reliable and scientific evidence for the clinical prevention of cervical cancer, it is of great value in development and application of HPV vaccine.%目的 分析衢州地区1280例宫颈病变女性中人乳头状瘤病毒(HPV) 21种基因型的分子流行病学特点.方法 对衢州地区1280例宫颈病变的女性进行21种HPV基因型分型检测,比较不同基因型的流行病学特点.结果 1280例宫颈病变病例中HPV感染708例,感染率为55.31%,其中高危型HPV感染率为38.83%,低危型HPV感染率为16.48%,高危型最常见类型为HPV16、58、52,低危型最常见类型为HPV6、11;31~40岁组HPV总感染率及高危型感染率最高,分别占67.39%和50.58

  17. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    Sara Vázquez-Corzo

    2003-10-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

  18. 人乳头瘤病毒基因型与宫颈病变的相关性分析%Correlation analysis of human papilloma virus genotypes and cervical lesions

    Institute of Scientific and Technical Information of China (English)

    黄秀珍; 姚心韵; 陈文学; 吕赛平

    2015-01-01

    目的:探讨不同基因型人乳头瘤病毒(human papilloma virus, HPV)感染与宫颈病变的相关性。方法采用导流杂交基因芯片技术(Hybrimax)对924例妇女进行21种HPV基因型检测,同期进行宫颈活检病理检查。按病理学诊断结果分成6组:宫颈鳞癌组287例;宫颈上皮内瘤变(CIN)Ⅲ组78例;CINⅡ组61例;CINⅠ组153例;宫颈湿疣组40例;正常或炎症组305例。结果⑴503例检测到HPV感染,阳性率为54.4%。所有标本共检测到19种HPV基因型,未检测到HPV43、44型。不同病变组HPV阳性率差异有统计学意义(P<0.05),宫颈鳞癌组与宫颈湿疣组HPV阳性率显著高于CINⅢ组(P<0.05),CINⅢ组HPV阳性率显著高于CINⅡ组与CINⅠ组(P<0.05),CINⅡ组与CINⅠ组HPV阳性率显著高于正常或炎症组(P<0.05)。⑵不同病变组HPV多重感染率差异有统计学意义(P<0.05),宫颈鳞癌组、CINⅢ组、CINⅡ组、CINⅠ组HPV多重感染率显著高于湿疣组与正常或炎症组(P<0.05)。结论不同类型宫颈病变与HPV感染率、HPV感染型别有着一定的关系, HPV多重感染可能是宫颈鳞癌以及宫颈上皮内瘤变的危险因素。%Objective To investigate the correlation between the infection of different genotypes of human papilloma virus (HPV) and cervical lesions. Methods Twenty-one kinds of HPV genotypes were detected in 924 cases of women by flow-through hybridization and gene chip technology, and the cervical biopsy was performed at the same time. According to the results of patho-logical diagnosis, these women were divided into 6 groups, including cervical squamous cell carcinoma group (287 cases), cervical intraepithelial neoplasia (CIN)Ⅲ group(78 cases), CINⅡ group(61 cases), CINⅠ group (153 cases), cervical condyloma group (40 cases), normal or inflammation group (305 cases). Results A total of 503 cases were HPV-positive, and the positive rate was 54.4%. 19 kinds of HPV genotypes

  19. 液基薄层细胞学检测在宫颈上皮内瘤变和宫颈癌诊断中的应用价值分析%The application of TCT in the diagnosis of cervical epithelial lesions and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    韩一栩; 曾新

    2015-01-01

    Objectives:To investigate the application value of TCT in the diagnosis of cervical epithelial le-sions and cervical cancer.Methods:681 patients that received cervical lesions screening in our department from June 201 2 to December 201 3 were selected and were tested by TCT and colposcopy cervical biopsy.The relationship between age and incidence rate was explored,and results of TCT cytological and histological diagnosis were com-pared.Results:The positive rate of TCT was higher in patients of 36 ~45 years old.There were ASC -H (60 ca-ses),ASC -US (222 cases),LSIL(89 cases),HSIC (1 4 cases),SCC (5 cases)and AGC (2 cases)diagnosed by TCT cytology,and 1 78 cases of CINI,1 1 4 cases of CIN II, 33 cases of CIN III and 1 3 cases of SCC diagnosed by TCT.Choosing histological diagnosis as the gold standard,the coincidence rate of ASC -H,ASC -US,LSIL, HSIC and SCC were 91 .67% (55 /60),34.23% (76 /222),87.64% (78 /89),92.86% (1 3 /1 4)and 1 00%(2 /2)respectively.Conclusion:With a high positive rate,TCT is an important screening means for cervical intra-epithelial lesions,which is conducive to early diagnosis and treatment of cervical intraepithelial neoplasia and cervi-cal carcinoma.%目的:探讨液基薄层细胞学技术(TCT)在诊断宫颈上皮内瘤变及宫颈癌中的应用价值。方法:选取2012年6月至2013年12月期间在我院接受宫颈病变筛查的681例患者作为研究对象,行 TCT检查及阴道镜下宫颈活检,探讨年龄与发病率的关系,同时对比研究 TCT 细胞学与组织学诊断结果。结果:TCT 阳性率多见于36~45岁年龄段;TCT 细胞学诊断 ASC -H 60例,ASC -US 222例,LSIL 89例,HSIC 14例,SCC 5例,AGC 2例;组织学诊断 CINI 178例,CINⅡ114例,CINⅢ33例,SCC 13例,以组织学诊断为金标准,ASC -H 符合率为91.67%(55/60),ASC -US 为34.23%(76/222),LSIL 为87.64%(78/89),HSIC 为92.86%(13/14),SCC 为100%(2/2)。

  20. p16INK4a在宫颈病变中的研究进展%Progress of P16INK4a in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    刘莹; 周瑾

    2011-01-01

    宫颈癌严重威胁女性健康和安全,是导致女性死亡的主要恶性肿瘤之一.近年来我国宫颈癌的发病率正以每年2%~3%的速度增长,因此早期诊断对于预防和治疗宫颈癌具有决定性的意义.p16INK4a是近年来发现的肿瘤抑制基因,是一种细胞周期蛋白D依赖性激酶的抑制剂,研究表明p16INK4a的表达与宫颈癌的发生发展密切相关.目前宫颈癌筛查中的细胞学检查及hr-HPV检测方法 都具有一定的局限性,寻求新的筛查方法 已成为研究热点.p16INK4a的检测技术易于普及、操作简便,不仅能提高宫颈癌的早期诊断率、预测宫颈癌的发生发展,且较HC-Ⅱ检测更能区分是否存在病变,从而降低传统宫颈癌筛查的假阳性率及假阴性率,提高筛查的灵敏度和特异度,为宫颈癌的筛查开辟了新途径,值得进一步深入研究.%Cervical cancer was a serious threat to women's health and safety , which was the leading cause of cancer death for women. In recent years,the incidence of cervical cancer increased 2% -3% per year in China,so an early diagnosis to prevent cervical cancer and the treatment was of decisive importance. As a tumor suppressor gene,pl6INK4a was a recently discovered inhibitor of cyclin D-dependent kinase; studies had shown that the expression of pl6INK4a was closely linked to the development of cervical cancer. Current screening of cervical cytology and detection methods hr-HPV had certain limitations,to seek new screening method had become a research hotspot.pl6INK4a detection technology was easy to spread,easy to use,which can not only improve the early diagnosis of cervical cancer to predict the impact of the development of cervical cancer, and was better than the HC- II test to distinguish the existence of the disease,thus reducing false positives traditional cancer screening cervical rate and false negative rates,and improving the sensitivity and specificity of screening for cervical

  1. 液基薄层细胞检测系统与阴道镜下活检在诊断子宫颈病变中的价值%Value of Liquid-Based Thin-Layer Cell Detection System and Cervical Biopsy Vaginoscopy in Diagnosis of Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    王美玲; 王薇; 周新娥; 吴逢霞; 蒋亚男

    2009-01-01

    Objective To study the diagnostic value of liquid-based thin-layer cell detction system(LCT)and cervical biopsy vagionscopy for cervical intraepithelial neoplasia(CIN).Method The clinical information of 7118 patients that received LCT and 778 cases of positive cytological result that received cervical biopsy were retrospectively analyzed.167 patients with negative result in cytological test received cervical biopsies vaginoscopy because of their high-grade clinical suspicion.Results In 7118 patients,429 cases was diagnosed as CIN by cervical biopsy,the diagnosis rate was 6.03%(429/7118).In 167 patients,78 cases of 778 cases that were suggested as untypical squamous cell lesions by LCT were diagnosed as CIN by cervical biopsy.The total diagnosis rate is 7.12%[(429+78)/7118].There was a significant difference between them(P<0.01).Conclusions LCT could detect cervical lesion in its early stage.The patients whose cytological test results are positive or clinical high-grade suspicious should receive cervical biopsy vaginoscopy,which may benefit to the increase of detection rate of CIN.%目的 探讨液基薄层细胞检测系统(LCT)联合阴道镜及阴道镜下宫颈活检对宫颈上皮内瘤变(CIN)的诊断价值.方法 回顾性分析7118例LCT筛查结果及其中778例细胞学阳性行阴道镜下活检的病理结果,167例虽细胞学阴性,但临床高度可疑,行阴道镜检出并取活检.结果 7118例患者中,778例LCT检查提示不典型鳞状细胞以上病变者,再行阴道镜下活检发现CIN以上病变429例,检出率为6.03%(429/7118).167例LCT检查阴性但临床高度可疑病例,行镜下活检CIN以上病变78例,总检出率为7.12%[(429+78)/7118].两者比较差异显著(P<0.01).结论 采用LCT筛查可早期发现宫颈病变,细胞学阳性或临床可疑者应配合阴道镜检查及镜下活检可提高CIN的检出率,但如果同时进行阴道镜检查可提高CIN的检出率.

  2. Towards a new understanding of cervical glandular epithelial lesions:pathological perspectives%宫颈腺上皮病变病理学相关问题及其研究进展

    Institute of Scientific and Technical Information of China (English)

    刘从容

    2016-01-01

    In May 2014, World Health Organization (WHO ) released WHO (2014 ) Classification of Tumor of the Female Reproductive Organs.According to the classification of tumors precursor lesion of cervical adenocarcinoma of female reproductive system in WHO (2014 ) Classification of Tumor of the Female Reproductive Organs,and the latest fundamental research progress of human papillomavirus (HPV)new developments,historical perspectives and controversial issues about the cervical glandular epithelial precursors will be presented.%结合2014年5月,世界卫生组织(WHO)发布的《WHO(2014)女性生殖系统肿瘤分类》中,宫颈腺癌前驱病变分类与人乳头状瘤病毒(HPV)感染最新基础研究进展,对宫颈腺上皮病变的相关病理学问题及其研究进展进行简要介绍。

  3. Infecção por Chlamydia em pacientes com e sem lesões intra-epiteliais cervicais Chlamydia infection in patients with and without cervical intra-epithelial lesions

    Directory of Open Access Journals (Sweden)

    Micheline de Lucena Oliveira

    2008-12-01

    and without intraepithelial cervical lesions attended at specialized ambulatory in Recife (2007 and its association with biologic and demographic variables, habits, reproductive, clinical and gynecologic characteristics. METHODS: A cross-sectional study was conducted including 70 women (35 with cytological cervical lesions and 35 with normal smears. Colposcopy and biopsy when necessary were performed and Chlamydia infection was determined by direct immunofluorescence. Analysis variables were age, race, precedence, schooling, marital status, menarche, age at first sexual intercourse, parity, number of sexual partners, vaginal discharge, previous Pap smear, STD episodes, cervical cauterization, contraceptive methods, familial antecedents of cervical cancer, alcohol intake, use of illicit and immunosuppressive drugs, Papanicolaou result and cervical Chlamydia l infection. To determine the strength of association prevalence ratio (PR and its 95% confidence interval were calculated. Multivariate analysis was performed for controlling potentially confounding variables. RESULTS: the frequency of Chlamydia l infection was significantly greater in patients with intraepithelial cervical lesions (80.0% vs. 14.3%; PR = 5.60; 95% CI = 2,44 - 12,82. When the factors associated with Chlamydia l infection were analyzed, the only variable that remained significantly associated after multivariate analysis was previous episode of STD (OR=63,47; 95% CI = 13,93 - 289,09. CONCLUSION: Chlamydia trachomatis infection is associated with intraepithelial cervical lesions and history of STD should be considered for treatment and follow-up of these patients.

  4. 女性不同人群宫颈病变发病状况调查及危险因素分析%Incidence and Risk Factors of Cervical Lesions among Different Famale People

    Institute of Scientific and Technical Information of China (English)

    徐又先; 袁林; 陈广莉

    2012-01-01

    Objective To investigate the incidence of cervical lesions in urban,non-resident and rural population of Liuzhou area and to analyze its relationship with living and sanitation habits. Methods Living and sanitation habit were examined using questionnaire and investigation of the high risk factor of the general condition, the result of gynecology physical examination,analysis of the risk factors and the pathological types of cervical lesions. Results Education level,using tubbing,number of sex partner,smoking, drinking,vaginal douching, washing vulva during menstruation, frequency of vitamin food consumption, were statistically different among three populations. The incidence of CIN was 6. 11%, 11.60% and 10. 37% .respectively. Nonresident and rural females were more popular in urban women, and HPV infection was highest in rural female (29. 0%). The difference of the incidences of HPV and CIN were found among three populations (P<0. 05). After multiple factor non-conditional Logistic regression analysis, early age of first intercourse,multiple sexual partner were the risk factors of cervical lesions. Smoking and drinking showed no correlation with cervical lesions. Conclusion Different living and sanitation habit of urban,rural and non-resident populations may contribute to the difference in incidence of CIN. Individual behavior intervene, especially douching forbidden should be done to reduce the prevalence of CIN.%目的 了解城市人群、流动人群、农村人群宫颈病变发病状况及不同生活、卫生行为对宫颈病变发病的影响.方法 对三组人群行宫颈病变调查和妇检,并行高危因素分析.结果 三组教育程度、盆浴、性伴侣数、吸烟、饮酒、阴道冲洗、经期清洗外阴和食维生素类食物情况差异有统计学意义;宫颈病变检出率分别为6.11%、11.60%、10.37%,流动和农村组高于城市组;HPV感染农村人群(29.0%)和流动人群(21.7%)明显高于城市人群(11.0

  5. 液基薄层细胞学检查配合阴道镜检查对子宫颈病变的诊断价值%The diagnostic value of thinprep cytology combined with colposcopy for cervical lesions

    Institute of Scientific and Technical Information of China (English)

    白杰

    2014-01-01

    Objective To investigate the diagnostic value of ThinPrep cytology(TCT) combined with colposcopy for cervical lesions.Methods 980 patients with suspected cervical lesions were selected and received TCT combined with colposcopy examination.Among the 980 patients,102 patients with positive smear were referred for colposcopy and were diagnosed by colposcopy biopsy.Results Of 102 patients with positive smear,the detection rates of the atypical squamous cells (ASC),low-grade squamous intraepithelial lesions (LSIL),cervical intraepithelial neoplasia (HSIL) and atypical glandular cells (AGC) were 6.1%,0.6%,2.1% and 1.5%,respectively.Compared with the colposcopy biopsy for cytology samples,the test results were as follows:LSIL was 72.4% (21/29),HSIL was 93.8% (15/16).Conclusion The method of TCT could increase the screening rate of positive diagnosis of cervical lesions and for the highly suspected patients,the combination of vaginal colposcopy and biopsy could further improve the accuracy of diagnosis,in order to provide scientific solutions for clinical treatment.%目的 探讨液基薄层细胞学检查(TCT)联合阴道镜检查对子宫颈病变的临床诊断应用价值.方法 选取疑似为宫颈病变的患者980例,采用TCT联合阴道镜检查,其中,送检样本阳性涂片患者102例,均行阴道镜检查,于阴道镜下取组织活检.结果 980例患者的102例阳性患者中,不典型鳞状上皮细胞(ASC)、低度鳞状上皮内病变(LSIL)、宫颈癌前病变(HSIL)及不典型腺细胞(AGC)的检出率分别为6.1%、0.6%、2.1%和1.5%;对细胞学阳性样本进行阴道镜组织活检,两种检测结果比较:LSIL为72.4% (21/29),HSIL为93.8% (15/16).结论 TCT筛查可增加宫颈病变阳性诊断率,对于高度疑似患者配合阴道镜检查及阴道组织活检可以进一步提高诊断的准确度,以便为临床治疗提供科学的方案.

  6. A 7-year randomized prospective study of a one-step self-etching adhesive in non-carious cervical lesions. The effect of curing modes and restorative material

    DEFF Research Database (Denmark)

    van Dijken, Jan W V; Pallesen, Ulla

    2012-01-01

    Objective: The aim of this study was to evaluate the clinical retention of a one-step self-etching adhesive system (Xeno III) in Class V non-carious cervical lesions and the effect of restorative material and curing techniques on longevity of the restorations. Material and methods: A total of 139......-start or a pulse-delay curing mode. The restorations were evaluated at baseline, 6, 12, 18 and 24 months and then yearly during a 7 years follow-up with modified USPHS criteria. Dentin bonding efficiency was determined by the percentage of lost restorations. Results: During the 7 years, 135 restorations could...... be evaluated. No post-operative sensitivity was reported by the participants. Overall relative cumulative loss rate frequencies for the adhesive system at 6, 18 and 7 years, independent of curing technique and restorative material, were 0.8%, 6.9% and 23.0%, respectively. The self-etching adhesive fulfilled...

  7. A deficit in biopsying potentially premalignant oral lesions in Puerto Rico

    OpenAIRE

    Morse, Douglas E.; Psoter, Walter J.; Cuadrado, Lumarie; Jean, Yves A.; Phelan, Joan; Mittal, Khush; Buxó, Carmen J; CRUZ, GUSTAVO D.; Elias, Augusto

    2009-01-01

    The study findings suggest that there is a substantial shortfall in the biopsying of potentially precancerous oral lesions in Puerto Rico. Persons living in Puerto Rico who have intraoral lesions suspicious for oral cancer or precancer are most likely to be biopsied only after developing an invasive cancer and therefore are not receiving the benefits of early detection.

  8. Expressions of Toll-like receptors 3, 4, 7, and 9 in cervical lesions and their correlation with HPV16 infection in Uighur women

    Institute of Scientific and Technical Information of China (English)

    Ayshamgu Hasimu; Lin Ge; Qiao-Zhi Li; Rui-Ping Zhang; Xia Guo

    2011-01-01

    Recent findings show that Toil-like receptors (TLRs) expressed in immune cells play a crucial role in the innate immune response and the subsequent induction of adaptive immune responses against microbial infection on tissue injury. Furthermore, expression of TLRs in cancer cells is associated with tumor proliferation and invasion. To explore the role of TLRs expression in cervical carcinogenesis in Uighur women, we detected the expressions of TLR3, TLR4, TLR7, and TLR9 in 25 normal cervical tissues, 64 cervical intraepithelial neoplasia (CIN) tissues, and 63 cervical squamous cell carcinoma (CSCC) tissues using immunohistochemical staining, as well as human papillomavirus type 16 (HPV16) infection using PCR. All samples used in this study were from Xinjiang Uighur women. We found the expression levels of TLR4, TLR7, and TLR9 were significantly higher in CIN and CSCC than in normal controls (P < 0.05). Up-regulation of TLR4 and TLR7 were correlated with tumor differentiation but not FIGO stage or lymph node metastasis (P > 0.05). Up-regulation of TLR9 was correlated with lymph node metastasis (P < 0.05) but not tumor differentiation or FIGO stage (P > 0.05). We also analyzed the correlation between the expressions of TLRs and HPV16 infection and found that the expressions of TLR4 and TLR9 significantly correlated with HPV16 infection in CIN (r = 7.434, P = 0.006; r = 7.123, P = 0.008) and CSCC (r = 6.423, P = 0.001; r = 8.478, P = 0.004), whereas the expression of TLR3 was not significantly different in any of the three groups and had no significant correlation with HPV16 infection. Our results suggest that high expression of TLR4, TLR7, and TLR9 may play important roles in the development and progression of ClN and CSCC in Uighur women, and the expressions of TLR4 and TLR9 can be up-regulated by HPV16 infection.

  9. 电子阴道镜检与TCT检测在宫颈病变筛查中的作用%Electronic Colposcopy and TCT Detected in The Screening of Scervical Lesions

    Institute of Scientific and Technical Information of China (English)

    高晓娅

    2012-01-01

      Objective:Explore digital colposcope and the membrane thin layer of liquid-based cytology (Thin-prep cytoLogy test,TCT) in the diagnosis of cervical disease, the clinical value. Methods:3980 cases of patients of colposcopy and TCT check and the results analyzed. Results:The electronic colposcopy chronic cervicitis, cervical polyps, genital warts and cervical cancer (cervical intraepithelial neoplasia-like lesions, CIN) CINⅠ, CIN II, CINⅢand pathological diagnosis coincidence rate of 94.1%, 100%, 93.5%, 92.3%, 81.4%, 79.9%and 54.1%;whole group of 3980 cases of TCT method and pathological examination method detection CINⅠof CIN II of CIN III and cervical cancer were 20.6%and 20.0%,. Both results are highly significant difference p<0.01 (x2=25.826). Conclusion:The digital colposcope and TCT on the diagnostic accuracy of cervical disease, especially the significance of cervical precancerous lesions and early cervical cancer screening.%  目的:探讨电子阴道镜和膜式薄层液基细胞学检查(Thin-prep cytoLogy test,TCT)在宫颈疾病诊断中的临床价值.方法:对3980例患者行电子阴道镜检查和TCT检查并对其结果进行分析.结果:电子阴道镜对慢性宫颈炎、宫颈息肉、尖锐湿疣、宫颈癌、(宫颈上皮内瘤样病变,CIN)CINⅠ、CINⅡ、CINⅢ的诊断与病理诊断符合率分别达94.1%、100.0%、93.5%、92.3%、81.4%、79.9%和54.1%;全组3980例TCT法和病理检查法检出CINⅠ、CINⅡ、CINⅢ和宫颈癌分别为17.4%和20.0%,二者结果比较有高度显著性差异p<0.01(x2=25.826).结论:电子阴道镜和TCT对宫颈疾病的诊断准确性高,尤其是对宫颈癌前病变和早期宫颈癌筛查具有重要意义.

  10. Screening results on cervical lesions with DNA quantitative cytology and liquid-based cytology%DNA定量细胞学配合液基细胞学对宫颈病变筛查的价值

    Institute of Scientific and Technical Information of China (English)

    曹红英; 武卫华; 许振; 许艳梅

    2011-01-01

    OBJECTIVE: To evaluate the value of the DNA quantitative cytology with liquid-based cytology in cervical cancer prevention and treatment. METHODS: 4 352 cases of patients in our hospital outpatient gynecologic from 01-01-2009 to 10-31-2010 were involved in this study. All the 504 cases which were recommended biopsy were conducted colposcopy and biopsy. The pathological changes were observed after the routine HE staining. Then the positive coincidence rate of the liquid-based cytology was calculated with routine HE staining and DNA Feulgen staining with routine HE staining respectively. RESULTS: The positive rate was 52. 18% (263/504) with the diagnosis of the TBS biopsy criteria. The positive rate was 66.67%(336/504) with the diagnosis of the DNA Feulgen staining biopsy criteria, While the positive rate was 81.75 % (412/504) with the diagnosis of the two combined cytological method biopsy criteria. There was significant difference among the three methods (P<0. 05). CONCLUSIONS: DNA Feulgen staining compared with the single liquid-based cytology, the rate of early detection of cervical lesions is improved significantly. The false negative rate of thc liquid-based cytology can be reduced by the combination of the two, but also the detection rate of cervical lesions of early can be improved. It plays a posive role in order to prevent further development of lesions of early cervical cancer.%目的:评价DNA定量细胞学配合液基细胞学检查在宫颈癌防治中的价值.方法:收集2009-01-01-2010-10-31在我院妇科门诊行液基细胞学及DNA定量检查的患者4 352例,对检查结果建议为活检的病例504例行阴道镜检查及活体组织检查,常规HE染色后观察病变程度,分别计算其与液基细胞学和DNA Feulgen染色后的阳性率.结果:以TBS活检标准行活检病例的阳性检出率为52.18%(263/504),以DNA定量分析结果活检标准行活检的病例阳性检出率为66.67%(336/504),经2种细胞学方法联

  11. Investigation of diagnosis and treatment method of cervical intraepithelial neoplasiaUAN Xiao-yan%探讨宫颈上皮内瘤样病变的诊断及治疗方法

    Institute of Scientific and Technical Information of China (English)

    袁小燕

    2014-01-01

    Cervical intraepithelial neoplasia (CIN) refers to continuously precancerous lesions process from cervical epithelial atypical hyperplasia to carcinoma in situ. In recent years, the morbidity rates of CIN and cervical cancer have increased, and the patients tend to be younger. Therefore, it is important to have CIN correctly diagnosed and properly treated. The traditional Pap smear, thinprep cytology test (TCT), examination of human papilloma virus (HPV), colposcopy test, and cervical biopsy are the main diagnosing methods of CIN. Due to the features of high accuracy, non-invasion, and repeatable operation, colposcopy is the major method in diagnosis of cervical lesions. The common CIN treatment methods include physical therapy and operation therapy. CO2 laser therapy, cryotherapy, and electric coagulation therapy are included in the physical therapy, and cervical conization and hysterectomy are the operation therapy. Loop electrosurgical excision procedure (LEEP) has become the main treatment method for precancerous lesions of cervical cancer and early-stage cervical cancer, because of its characteristics of short treatment time, simple operation, less damage, deeper and wider range of lesions treatment, and pathological examination of the resected tissues.%宫颈上皮内瘤样病变(CIN)是指子宫颈上皮非典型增生至原位癌这一系列癌前病变的连续过程,近年CIN及宫颈癌的发病率增加且患者年龄趋于年轻化,因此及时正确诊断CIN并得到合适的治疗尤为重要。CIN的诊断有传统巴氏涂片法、液基细胞学(TCT)检查、人乳头瘤病毒(HPV)检查、阴道镜检查、宫颈活检等多种方法,其中阴道镜因其准确性高、无创伤、可重复操作等特点,是目前诊断宫颈病变的重要方法。常用的CIN治疗方法有物理治疗和手术治疗。物理治疗方法包括CO2激光治疗、冷冻治疗、电凝治疗等。手术治疗包括宫颈锥切术和全子宫切除术等

  12. Lesões precursoras do câncer cervicouterino: evolução histórica e subsídios para consulta de enfermagem ginecológica Lesiones precursoras del cáncer cervical-útero: evolución histórica consolidando la consulta de enfermería ginecológica Cervical-uterine cancer precursor lesions: historical evolution supporting the gynecological nursing consultation

    Directory of Open Access Journals (Sweden)

    Maria Cristina de Melo Pessanha Carvalho

    2010-09-01

    Full Text Available Estudo emergido de recorte de dissertação de mestrado, ilustrando a evolução histórica das lesões precursoras do câncer cervicouterino (LPCCU. Trata-se da história das LPCCU, delineando a relevância do conhecimento para prática do enfermeiro na área da saúde da mulher. O conceito de LPCCU inicia-se a partir do século XIX, dando início aos estudos das células alteradas. Objetivo: descrever as diversas fases da evolução histórica das alterações cervicais. Estudo qualitativo, descritivo-analítico, recorte temporal no período de 1940 a 2008. Dados levantados mediante bibliografia de fonte primária e recurso BIREME. Pontuaram-se as classificações que já existiram, destacando a Nomenclatura Brasileira, importante para corresponder às necessidades e o perfil da saúde das mulheres do Brasil. Este estudo é o ponto de partida para respaldar as práticas de consulta de enfermagem ginecológica com abordagens educativas, contemplando a população feminina em ações preventivas e incentivo ao tratamento.Estudio emergido de recorte de disertación de máster, ilustrando la evolución histórica de las lesiones precursoras del cáncer cervical-uterino (LPCCU. Se trata de la historia de las LPCCU, delineando la relevancia del conocimiento para la práctica del enfermero en cuidados en la salud de la mujer. El concepto de LPCCU se inicia a partir del siglo XIX, empezando los estudios de las células alteradas. Como objetivo: describir las diversas fases históricas de las alteraciones cervicales. Estudio cualitativo, descriptivo-analítico, recorte temporal en el período de 1940 hasta 2008. Datos acrecentados mediante bibliografía de fuente primaria y recurso BIREME. Se puntuó las diferentes clasificaciones que ya existieron, conde destaque para la nomenclatura brasileña, importante para corresponder a las necesidades y el perfil de la salud de las mujeres del Brasil. Esto estudio es el ponto de partida para respaldar las pr

  13. Application of colposcopy in diagnosing cervical intraepithelial neoplasia and cervical cancer%阴道镜检查在宫颈上皮内瘤变及宫颈癌诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    袁靖君

    2012-01-01

    Objective: To explore the value of colposcopy in the diagnosis of cervical diseases. Methods; A total of 5,833 patients were examined with Thinprep Cytologic Test(TCT) in the Third Hospital of Datong Colliery Corporation, Shanxi Province from Jul. 2006 to Oct. 2010. Two hundred and sixty-three patients with TCT positive were selected to do the electronic colposcopy test and the pathological examination of biopsy at the same time. Results: Among two hundred and sixty-three patients with TCT positive and cervical lesions, there were 37 cases of chronic cervicitis; 181 cases of cervical intraepithelial neoplasia (CIN) including 93 cases of CINⅠ, 50 cases of CINⅡ and 38 cases of CINⅢ; and 45 cases of cervical cancer according to colposcopy examination diagnosis. There were 36 cases of chronic cervicitis; 180 cases of CIN including 97 cases of CINⅠ, 46 cases of CINⅡ and 37 cases of CINⅢ; and 47 cases of cervical cancer according to the pathological examination results. Colposcopy examination in the diagnosis of CIN and cervical cancer showed that sensitivity was 97. 8%, specificity 94. 4%, accuracy 92. 2%, positive predictive 99. 1% and negative predictive 91. 9%. For patients with cervical cancer and CIN, the diagnostic accordance rate with colposcopy and pathological examination was more than 90%. Conclusions: Colposcopy has the great value in early diagnosis and treatment of cervical precancerous lesions, reducing the cervical cancer incidence.%目的 探讨阴道镜检查在宫颈疾病诊断中的应用价值. 方法 对2006年7月至2010年10月我院5,833例进行阴道脱落细胞液基薄层细胞学检查(TCT)呈阳性的患者263例行电子阴道镜检查,并在阴道镜下行定点多点宫颈活组织病理学检查. 结果 263例TCT阳性宫颈病变患者中,阴道镜检查诊断结果为慢性宫颈炎37例;宫颈上皮内瘤变(CIN)181例,其中CIN Ⅰ 93例,CIN Ⅱ 50例,CIN Ⅲ 38例;宫颈癌45例.经宫颈活组织病理学检查

  14. Utilidad en la combinación de oligonucleótidos universales para la detección del virus del papiloma humano en cáncer cervicouterino y lesiones premalignas Usefulness of combining universal oligonucleotides in detecting human papillomavirus in cervical cancer and premalignant lesions

    Directory of Open Access Journals (Sweden)

    Adela Carrillo

    2004-02-01

    Full Text Available OBJETIVO: Determinar la frecuencia y distribución del virus del papiloma humano en los diferentes estadios que conforman la historia natural del cáncer cérvico uterino, y optimizar la detección mediante el uso de diferentes oligonucleótidos universales. MATERIAL Y MÉTODOS: Se trata de un estudio transversal, descriptivo, en el que las muestras fueron colectadas durante enero a diciembre de 1999. El procesamiento de las muestras y el análisis de los datos se realizaron en el Instituto Nacional de Cancerología en la Ciudad de México. Se hizo análisis comparativo con t de Student para valores continuos y con ji cuadrada para proporciones, y análisis de concordancia entre biopsia y exudado cervical con la prueba estadística de Kappa. Para la detección del virus se utilizó la técnica de la reacción en cadena de la polimerasa (PCR con oligonucleótidos universales los cuales reconocen diferentes regiones del gen L1 (MY09/11; GP5/6; L1C1/2, y oligonucleótidos específicos para el VPH 16 y el VPH 18, así como secuenciación directa de los productos de la PCR. RESULTADOS: Se analizaron 154 muestras: 65 (42.2% citologías normales, 45 (29.2% lesiones de alto y bajo grado, y 44 (28.6% de cáncer invasor. El VPH fue detectado en 95.5% de los casos de cáncer invasor, en 91.6% de lesiones de alto grado, en 66.7% de lesiones de bajo grado y en 23.1% de citologías normales, por la PCR con al menos uno de los juegos de oligonucleótidos utilizados. La detección fue más eficiente en las muestras obtenidas por biopsia que en los exudados cervicovaginales. El porcentaje total de detección del VPH con un juego de oligonucleótidos universales (37.6% aumentó sustancialmente (60.4% al combinarlo con otros dos juegos de oligonucleótidos universales. CONCLUSIONES: La presencia del VPH de alto riesgo es elevada inclusive en mujeres con epitelios cervicales con diagnóstico citológico normal. La detección del VPH mejora al utilizar distintos

  15. HPV prevalence and type-distribution in cervical cancer and premalignant lesions of the cervix: A population-based study from Northern Ireland.

    Science.gov (United States)

    Anderson, Lesley A; O'Rorke, Michael A; Wilson, Robbie; Jamison, Jackie; Gavin, Anna T

    2016-07-01

    Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV genotypes predominating in pre-malignant and cervical cancers in Northern Ireland (NI) before the vaccination campaign has effect. Formalin fixed paraffin embedded tissue blocks from 2,303 women aged 16-93 years throughout NI were collated between April 2011 and February 2013. HPV DNA was amplified by PCR and HPV genotyping undertaken using the Roche(®) linear array detection kit. In total, 1,241 out of 1,830 eligible samples (68.0%) tested positive for HPV, with the majority of these [1,181/1,830 (64.5%)] having high-risk (HR) HPV infection; 37.4% were positive for HPV-16 (n = 684) and 5.1% for HPV-18 (n = 93). HPV type-specific prevalence was 48.1%, 65.9%, 81.3%, 92.2%, and 64.3% among cervical intraepithelial neoplasias (CIN) Grades I-III, squamous cell carcinomas (SCC) and adenocarcinoma (AC) cases, respectively. Most SCC cases (81.3%) had only one HPV genotype detected and almost a third (32.0%) of all cervical pathologies were HPV negative including 51.9% of CIN I (n = 283), 34.1% CIN II (n = 145), 18.7% of CIN III (n = 146), 7.8% of SCC (n = 5), and 35.7% of AC (n = 5) cases. This study provides important baseline data for monitoring the effect of HPV vaccination in NI and for comparison with other UK regions. The coverage of other HR-HPV genotypes apart from 16 and 18, including HPV-45, 31, 39, and 52, and the potential for cross protection, should be considered when considering future polyvalent vaccines. J. Med. Virol. 88:1262-1270, 2016. © 2015 Wiley Periodicals, Inc. PMID:26680281

  16. Correlative analysis between abnormal cervical cytology and pathology of vaginoscopic biopsy or conization

    Institute of Scientific and Technical Information of China (English)

    Li Cai-juan; Lang Jing-he; Cheng Xue-mei; Wang You-fang

    2006-01-01

    Objectives: To evaluate the predictive value of cervical intraepithelial neoplasia (CIN)Ⅲ/ carcinoma in situ (CIS) by correlating analysis between abnormal cervical cytologic findings and pathological diagnosis of vaginoscopic biopsies or conization.Methods: Routine cervical cytology screening was performed in 31,634 cases by fluid-based thin-layer method (ThinPrep cytology test, TCT), 948 patients had both abnormal squamous cell appearance by TCT and pathological diagnosis of vaginoscopic biopsies and /or cervical conization. The predictive value of CINⅢ/CIS were studied retrospectively by correlating analysis of different cytology abnormalities and pathology diagnosis.Results: Cytologically, 1,260 out of 31,634 TCT tests showed abnormal squamous cells appearance, including atypical squamous cell of undetermined significance (ASCUS) 675 cases(2.13%), low squamous intraepithelial lesion (L-SIL) 379 cases(1.20%), high squamous intraepithelial lesion (H-SIL)176 cases(0.56%),cancer 30 cases (0.09%). Among 948 patients with pathological diagnosis, there were CINⅡ-Ⅲ in 70 cases(7.38%) and CINⅢ/CIS in 56 cases.(5.91%). The relative risks (RR) of different precancerous TCT results in predicting CIN Ⅲ/CIS validated by pathology are as follow: AUSCUS 14.7% (95% confidence interval (CI) 8.0-27.0), Lsil 13.9% (95% CI 6.3-30.9), Hsil 126.1 (95% CI 60.6-218.6). The RR of AUSCUS group is not significantly different from that of L-SIL group(P =0.951). However, the RR of CINⅢ/CIS morbidity between the H-SIL or cancer group and the ASCUS or L-SIL group are significantly different (all P value <0.01).Conclusion: Vaginoscopic biopsy could verify pathological CINⅡ-Ⅲ and CINⅢ/CIS from cases with abnormal TCT results. There is significantly greater risk of being CINⅢ/CIS validated by vaginoscopic biopsy in the H-SILpatients,while ASCUS and L-SIL group have the coequal risk.

  17. Analysis of cervical diseases in 2 333 married women aged 35-59 years%2333例35~59岁已婚妇女宫颈疾病分析

    Institute of Scientific and Technical Information of China (English)

    梁海虹; 徐旦; 吴美飞

    2015-01-01

    Objective To understand the incidence status of cervical diseases among rural (fishery) women in Xiangshan area, so as to improve the early diagnosis and treatment of cervical cancer and improve the self health care consciousness and health level of rural ( fishery) women.Methods Cervical cells were collected by pelvic examination to perform liquid based thin-layer cytology test ( TCT) , and colposcopy examination, HPV test and histopathological examination were further performed for cases with abnormal cytological results. Results Gynecological examination showed that 57.35%of rural ( fishery) women were diagnosed with cervical diseases and the incidence of cervical erosion was highest (21.35%).TCT examination revealed that the incidence of abnormal cervical cytology results, precancerous lesions and cervical cancer was 3.26%, 120/10 000 and 8.57/10 000, respectively.Conclusion TCT diagnosis revealed by TBS reporting system was superior to traditional cervical smear pap cytology report in detecting of precancerous lesions.Women of childbearing age ranging 40-50 years are high-risk group developing cervical cancer, and the cases with persistent infection of HPV are the objects of key intervention and testing.Strengthening rural ( fishery ) women ’ s health education and improving their self health care consciousness will contribute to the early prevention of cervical disease.%目的:了解象山海岛地区农(渔)村妇女宫颈疾病的发病状况,提高宫颈癌的早诊和早治率,提升广大农(渔)村妇女的自我保健意识和健康水平。方法采用妇科内诊检查,采集宫颈细胞进行液基薄层细胞学检查( TCT),对细胞学检查异常的患者进一步行阴道镜检查、人乳头瘤病毒( HPV)检测、宫颈组织病理学检查。结果通过妇科检查发现57.35%的农(渔)村妇女患有宫颈疾病,其中以宫颈糜烂发病率最高(21.35%);通过TCT检查发现

  18. Folate receptor and Ki-67 nucleoprotein expressions in cervical cancer tissue and their correlation

    Institute of Scientific and Technical Information of China (English)

    Ran Yan; Feng Li

    2016-01-01

    Objective:To detect the expression of both FR-α protein and ki-67 in cervical cancer tissues, and discuss the relationship between them and clinical significance.Methods:Using immunohistochemical method test normal cervical tissue and cervical cancer tissue before FR-α protein expression and the expression of Ki-67.Results:FR- protein expression in normal cervical tissues was positive for 7.0% while in cervical cancer tissue the positive rate was 82.1%. The difference was statistically significant. Ki-67 protein expression in normal cervical tissues was 0% while in cervical cancer tissue the positive rate was 80.2%. The difference was statistically significant. The two protein expression in cervical cancer stageⅠ,Ⅱ and stageⅢ were different, but the difference was not statistically significant. In cervical cancer tissues, both the two protein were positively correlated. There are correlations between them. Difference was statistically significant.Conclusion:FR-α elevated protein expression is involved in the pathogenesis of cervical cancer. FR-α protein expression in cervical cancer and precancerous tissue has correlation with Ki-67, FR-α protein maybe participate in the occurrence and development of the cell proliferation in cervical cancer.

  19. 两种排龈方法在楔状缺损修复中的应用研究%Study of two gingival retraction techniques used in restoring cervical abrasion lesions

    Institute of Scientific and Technical Information of China (English)

    王聪

    2012-01-01

    Objective To investigate the influence of two gingival retraction techniques on the periodontium and clinical performance before the restoration of cervical lesion. Methods 150 teeth with cervical lesion of 50 patients were selected. The teeth were divided into 3 groups randomly. In the experiment group, a conventional retraction cord ( Ultrapaks) and a paste-like material (Expasyls) were applied on the buccal aspects of each subject. In the control group, no gingival retraction techniques was applied before restoration and filled directly. Bleeding on probing and the pain were assessed after application. The retention, marginal adaptation, the presence of secondary caries and the GI after one year were evaluated . Data were analysed using Chi-Square and Kruskal - Wallis tests (a = 0. 05). Results There were significant difference between the rates of subjective feeling of the Ultrapaks and Expasyls. Bleeding was only induced by Ultrapaks in five subjects. After one year, the differences between the experimental and control groups in GI and marginal adaptation were of statistical significance ( P < 0.05 ). While there were no significant difference between the two control groups. The retention of the restoration and the presence of secondary caries were not statistically significant among the 3 groups. Conclusion Utilization of gingival retraction techniques before the restoration of cervical lesions can efficiently improve the quality of the restoration and the two gingival retraction methods were not statistically significant. The cordless gingival retraction technique is more easy, feasible, rapid than the retraction cord.%目的 研究在楔状缺损修复前使用两种排龈方法对其牙周组织及充填效果的影响.方法 选择50例全口至少有3个楔状缺损位于龈缘或龈下的患者(150颗患牙),每例患者的患牙随机分为3组,对照组直接树脂修复,实验组分别用ULTRA排龈线,Expusyl排龈膏排龈后再充填.分别

  20. [European guideline for the management of precancerous conditions in the stomach and it's application to Japan].

    Science.gov (United States)

    Haruma, Ken; Kamada, Tomoari; Murao, Takahisa; Yamanaka, Yoshiyuki; Ohsawa, Motoyasu; Shiotani, Akiko; Inoue, Kazuhiko

    2013-08-01

    Atrophic gastritis, intestinal metaplasia, and Helicobacter pylori (H. pylori) infection are commonly recognized as the risk factor of gastric cancer. In Japan mass screening by the X-ray examination or endoscopy has been performed for a long time in general population or in work place because of the high death rate and high incidence of gastric cancer. Periodic endoscopy has been recommended for the subjects with atrophic gastritis and/or intestinal metaplasia to detect gastric cancer in early stage. On the other hand, there was no guideline to manage premalignant conditions such as atrophic gastritis, intestinal metaplasia, and dysplasia in foreign countries. Recently the guideline for the management of precancerous conditions and lesions in the stomach (MAPS) has been published by the combined efforts of the European Society of Gastrointestinal Endoscopy, European Helicobacter Study Group, European Society of Pathology, and the Sociedade Portuguesa de Endoscopia Digestiva. In this article the main statements have been discussed on comparing the understandings as the premalignant conditions in Japan.

  1. A evaluation on cervical intraepithelial lesion and cervical cancer screening by DNA quantitative analysis and liquid-based monolayer cytology%液基薄层细胞学联合DNA定量方法对宫颈病变诊断试验的评价

    Institute of Scientific and Technical Information of China (English)

    侯安丽; 张玉娟; 李秀芬; 邵雪斋; 王杏茶

    2012-01-01

    mistake diagnostic rate,omission diagnostic rate and negative likelihood ratio arc lower than liquid-based monolaycr cytology. The combining sensitivity and the combining specificity of parallel tests between DNA quantitative analysis and liquid-based monolaycr cytology were 99. 56%、44. 52% respectively. The combining sensitivity and the combining specificity of serial tests between the two methods were 83. 78% \\89. 61 %, respectively. The sensitivity of parallel tests is the highest, The specificity of serial tests is the highest. Conclusion Liquid-based monolaycr cytology combined with quantitative DNA analysis may increase the sensitivity and specificity of cervical prccanccrous lesions and early cervical carcinoma.

  2. Cervical myositis ossificans traumatica: a rare location

    Energy Technology Data Exchange (ETDEWEB)

    Baysal, T.; Sarac, K.; Kutlu, R. [Dept. of Radiology, Inonu University, Malatya (Turkey); Baysal, O.; Ersoy, Y. [Dept. of Physical Therapy and Rehabilitation, Inonu Univ., Malatya (Turkey); Elmali, N. [Dept. of Orthopedics and Traumatology, Inonu Univ., Malatya (Turkey)

    1999-05-01

    An unusual case of myositis ossificans traumatica lesion located in the paraspinal region is reported. Despite the contiguity of the lesion with the cervical vertebrae and ominous appearance of the biopsy material, the history of antecedent trauma and computed tomography findings allowed preoperative accurate diagnosis. To our knowledge, myositis ossificans traumatica located in the cervical paraspinal region is very rare. (orig.) With 4 figs., 16 refs.

  3. 5-type HPV mRNA versus 14-type HPV DNA test: test performance, over-diagnosis and overtreatment in triage of women with minor cervical lesions

    OpenAIRE

    Westre, Bjørn; Giske, Anita; Guttormsen, Hilde; Sørbye, Sveinung Wergeland; Skjeldestad, Finn Egil

    2016-01-01

    Background Repeat cytology and HPV testing is used in triage of women with minor cytological lesions. The objective of this study was to evaluate 14-type HPV DNA and 5-type HPV mRNA testing in delayed triage of women with ASC-US/LSIL. Methods We compared a DNA test (Roche Cobas 4800) and an 5-type mRNA test (PreTect HPV-Proofer). In total 564 women were included in the study. Results The sensitivity among solved cases for CIN3+ were 100 % (15/15) for both tests. The sensitivity for CIN2+ of t...

  4. Expresi??n de ant??genos HLA clase I e infecci??n por papilomavirus humano tipo 16 en la neoplasia cervical uterina y lesiones precursoras

    OpenAIRE

    Torres Garc??a, Luis Manuel

    1992-01-01

    Sobre una casu??stica de 109 diagn??sticos de diferentes entidades nosologicas de cervix uterino, se determina la presencia de ant??genos hla clase i mediante la t??cnica de la inmunoperoxidasa, encontrando que tanto el epitelio normal como los condilomas y las lesiones intraepiteliales conservan la expresi??n de estos ant??genos, mientras que los tumores invasores pierden esta capacidad en el 15% de los casos, correlacion??ndose con la invasi??n del estroma y un aumento de su malignidad. Se ...

  5. PA03.11. Evaluation of socioeconomic status and other factors with special reference to cervical cancer A survey based study

    Science.gov (United States)

    Panwar, Aruna

    2013-01-01

    Purpose: Cervical cancer is cancer that starts in the cervix, the lower part of the uterus (womb) that opens at the top of the vagina. It is the third most common type of cancer in women. Cervical cancers start in the cells on the surface of the cervix. Most cervical cancers are from squamous cells. It starts as a precancerous condition called dysplasia. This precancerous condition can be detected by a Pap smear and is 100% treatable. It can take years for precancerous changes to turn into cervical cancer. The Papanicolaou test (also called Pap smear, Pap test, cervical smear, or smear test) is a screening test used to detect potentially precancerous and cancerous processes in the endocervical canal (transformation zone) of the female reproductive system. The test aims to detect potentially precancerous changes (called cervical intraepithelial neoplasia (CIN) or cervical dysplasia), which are usually caused by sexually transmitted human papillomaviruses. The present study was planned in the direction to evaluate. Method: In the present work, overall 50 patients of age group between 2050 yrs were selected to study their abnormal pap smear reports in relation to socioeconomic status, smoking, parity, and oral contraceptive and their relationship with abnormal cellular changes. So the patients were randomly selected for Pap smear from IPD or OPD of prasuti tantra and stri roga department of sdmca Hassan. Result: Out of 50 patients,10 patients belongs to higher class, 20 patients belongs to middle class,10 patients belongs lower middle class and 10 patients belongs to lower class. Conclusion: Cervical cancer is ranked as the most frequent cancer in women in India. India has a population of approximately 365.71 million women above 15 years of age, who are at risk of developing cervical cancer. Getting regular Pap smears can help detect precancerous changes, which can be treated before they turn into cervical cancer. Pap smears effectively spot such changes, but they

  6. Liquid-based Cytology with Colposcopy in the Clinical Diagnostic Application of Cervical Lesions%液基细胞学联合阴道镜检诊断宫颈病变

    Institute of Scientific and Technical Information of China (English)

    陈宁静; 涂干卿

    2010-01-01

    目的 研究液基薄层细胞学(TCT)检查联合阴道镜检对宫颈病变诊断的价值.方法 选择1 407例患者进行TCT、阴道镜检查和活组织病理检查.结果 281例单独行阴道镜检和699例单独行TCT检查提示活检的,分别有39.50%和42.63%属CIN Ⅰ级以上病变;427例TCT检查结合阴道镜检提示活检的,有86.42%属CIN Ⅰ级以上病变.结论 TCT检查结合阴道镜检定位活检可提高宫颈病变的准确诊断率.%Objective Studying the clinical diagnostic value of liquid-based thin-layer cytology(TCT)combined colpoacopic examination for cervical lesions.Methods In May 2006~August 2007 choose the 1407 patients coming the hospital who was done cytology examination(TCT)and coiposcopy examination and biopsy.Results Among 281 cases of eolposcopy prompt biopsy 39.50% of them suffered from CIN Ⅰ.So did 42.63 % of 699 cases of TCT examinations.427 cases of TCT combined Colposcopy were prompted they need to do biopsy.86.42% of them suffered from CIN Ⅰ or above lesions.Conclusion TCT combination of colposcopy and endoscopic biopsy can increase the rate of accurate diagnosis of cervicallesions.

  7. Cervical radiculopathy.

    Science.gov (United States)

    Iyer, Sravisht; Kim, Han Jo

    2016-09-01

    Cervical radiculopathy is a common clinical scenario. Patients with radiculopathy typically present with neck pain, arm pain, or both. We review the epidemiology of cervical radiculopathy and discuss the diagnosis of this condition. This includes an overview of the pertinent findings on the patient history and physical examination. We also discuss relevant clinical syndromes that must be considered in the differential diagnosis including peripheral nerve entrapment syndromes and shoulder pathology. The natural history of cervical radiculopathy is reviewed and options for management are discussed. These options include conservative management, non-operative modalities such as physical therapy, steroid injections, and operative intervention. While the exact indications for surgical intervention have not yet been elucidated, we provide an overview of the available literature regarding indications and discuss the timing of intervention. The surgical outcomes of anterior cervical decompression and fusion (ACDF), cervical disc arthroplasty (CDA), and posterior cervical foraminotomy (PCF) are discussed. PMID:27250042

  8. The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions.

    Science.gov (United States)

    Nuovo, Allison J; Garofalo, Michela; Mikhail, Alexandria; Nicol, Alcina F; Vianna-Andrade, Cecilia; Nuovo, Gerard J

    2013-09-01

    Formalin-fixed, paraffin-embedded tissues are widely used in biomedical research but little is known about the effect of the age of the block or unstained slides on the in situ hybridization or immunohistochemistry signal. We compared the in situ-based and immunohistochemistry-based signals for cervical intraepithelial neoplasia samples that ranged from 0 to 15 years of age. There was a progressive and statistically significant decrease in the strength of the p16 signal when comparing tissues prepared from recent unstained slides (0 to 1 y old, mean score of 92%) to those of intermediate age (5 to 7 y old, mean score of 49%) to old unstained slides (cut 13 to 15 y ago, mean score of 10%). Equivalent, progressive, and significant decreases in the intensity of the signals for microRNAs, CD45, and human papillomavirus DNA were seen in tissues stored on slides from 5 to 7 years and 13 to 15 years, respectively. However, the diminution of signal was much less, although still statistically significant, if the sections from the 13- to 15-year-old paraffin blocks were prepared in 2012. The data likely does not represent degradation of the targets as extraction of several microRNA from the old blocks showed no detectable degradation, despite the markedly weakened in situ hybridization signal. It is concluded that in situ-based signal for DNA, microRNAs, and proteins in paraffin-embedded tissues are significantly reduced over time, especially when stored long term on glass slides which, in turn, can lead to a significant underestimation of the amount and presence of the nucleic acid or protein target.

  9. 液基细胞结合阴道镜下活钳在宫颈病变中的应用%Application of Liquid Based Cytology Combined with Colposcopy Biopsy in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    税锦华; 李晓冬

    2014-01-01

    目的:了解液基细胞结合阴道镜下活钳对诊断宫颈病变的临床价值。方法对我院2010年1月1日至2013年12月31日妇科门诊自愿作TCT的6718例已婚妇女作TCT检测,结果异常的再作阴道镜活钳。TCT检查:用特制的塑料刷在宫颈内口磷柱状上皮交界处旋转6~8周,放入液基细胞保存液瓶中,经自动制片机制成簿片,经巴氏染色封片,由专业病检医生读片。阴道镜检查由专业从事阴道镜的医生,在子宫颈转化区3、6、9、12点处活钳或采用醋酸涂宫颈在醋酸白上皮区取材行宫颈活钳。