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Sample records for ceruloplasmin

  1. Study on the synthesis of ceruloplasmin as a functional radioprotector

    International Nuclear Information System (INIS)

    Ceruloplasmin is a normal, copper-containing plasma protein that has extracellular antioxidant properties in human and mammals. The in vivo physiological function has not yet been clarified, however, it has been proposed that ceruloplasmin may have a role in oxidation of Fe2+ to Fe3+ (ferroxidase) and promotion of lipoprotein oxidation under some circumstances. In this investigation, in addition to the many already known functions of ceruloplasmin, we would like to propose that ceruloplasmin has a thio-linked peroxidase function. The results are followings. 1) Human ceruloplasmin exhibited different antioxidant effects according to the electron donors in a metal-catalyzed oxidation system. 2) Purified ceruloplasmin did not plat a significant role in the protection of DNA stand breaks in the ascorbate/Fe3+/O2 system. However, when ascorbate were replaced with a thiol-reducing equivalent such as dithiothreitol, DNA strand breaks were significantly prevented by the same amount of ceruloplasmin. 3) Human ceruloplasmin showed a potent peroxidase ability to destroy H2O2 in the presence reduced glutathione. 4) Structural integrity may be needed for the glutathione-linked peroxidase activity of human ceruloplasmin. Intact human ceruloplasmin has a potent peroxidase properties to decompose H2O2 in the presence of reduced glutathione

  2. Study on the synthesis of ceruloplasmin as a functional radioprotector

    Energy Technology Data Exchange (ETDEWEB)

    Kim, In Gyu; Kim, Kug Chan; Shim, Hae Won; Lee, Chang Woo; Park, Hyo Kook; Park, Seon Young

    1998-12-01

    Ceruloplasmin is a normal, copper-containing plasma protein that has extracellular antioxidant properties in human and mammals. The in vivo physiological function has not yet been clarified, however, it has been proposed that ceruloplasmin may have a role in oxidation of Fe{sup 2+} to Fe{sup 3+} (ferroxidase) and promotion of lipoprotein oxidation under some circumstances. In this investigation, in addition to the many already known functions of ceruloplasmin, we would like to propose that ceruloplasmin has a thio-linked peroxidase function. The results are followings. 1) Human ceruloplasmin exhibited different antioxidant effects according to the electron donors in a metal-catalyzed oxidation system. 2) Purified ceruloplasmin did not plat a significant role in the protection of DNA stand breaks in the ascorbate/Fe{sup 3+}/O{sub 2} system. However, when ascorbate were replaced with a thiol-reducing equivalent such as dithiothreitol, DNA strand breaks were significantly prevented by the same amount of ceruloplasmin. 3) Human ceruloplasmin showed a potent peroxidase ability to destroy H{sub 2}O{sub 2} in the presence reduced glutathione. 4) Structural integrity may be needed for the glutathione-linked peroxidase activity of human ceruloplasmin. Intact human ceruloplasmin has a potent peroxidase properties to decompose H{sub 2}O{sub 2} in the presence of reduced glutathione.

  3. Correlation of serum ceruloplasmin with preeclampsia

    OpenAIRE

    Ghaseminejad A; Khodadai N; Nouri jaliani K; Shariat M; Hoshemian E

    2009-01-01

    "nBackground: Preeclampsia is a disorder of pregnancy with increased maternal and perinatal morbidity and mortality. An imbalance between free radical induced lipid peroxidation and antioxidant system has been suggested as possible pathogenesis of preeclapsia. It has been shown correlation of some serum antioxidant agents (for example ceruloplasmin) with preeclampsia and its severity. The purpose of this study was to evaluate of this correlation. "nMethods: In a analytic case-contro...

  4. Evaluation of serum ceruloplasmin in aggressive and chronic periodontitis patients

    OpenAIRE

    Harshavardhana, B.; Rath, S K; Mukherjee, Manish

    2013-01-01

    Background: Pro-inflammatory markers are seen to increase in inflammatory diseases like periodontitis. Detecting an increase in these markers is one of the diagnostic modality. One such marker, which can be detected, is the ceruloplasmin. Ceruloplasmin induces hypoxia and generates oxygen radicals at the site of aggressive periodontitis. It also causes a state of hypoferremia leading to increase in the natural resistance of the body. The aim of this study was to evaluate the serum levels of c...

  5. Ceruloplasmin and serum MDA levels in hypothyroid patients

    OpenAIRE

    Bhattacharya, Aruna; Saha, Rama; Mondal, Tridbeshwar; Choudhuri, Subhadeep; Gupta, Soma

    2014-01-01

    The present study was designed to investigate the relationship between serum levels of MDA which is a marker of oxidative stress and Ceruloplasmin with thyroid hormone status in hypothyroid females pre &posttreatment.The study group comprised of 46 patients with primary hypothyroidism, The patients were reevaluated after 6 months of L-thyroxine therapy. The patients were compared with equal number of normal healthy controls. Serum MDA and Ceruloplasmin were measured according to an enzyma...

  6. Diagnostic Value of Ceruloplasmin in the Diagnosis of Pediatric Wilson's Disease

    OpenAIRE

    Kim, Jung Ah; Kim, Hyun Jin; Cho, Jin Min; Oh, Seak Hee; Lee, Beom Hee; Kim, Gu-Hwan; Choi, Jin-Ho; Kim, Kyung Mo; Yoo, Han-Wook

    2015-01-01

    Purpose Measurement of serum ceruloplasmin level is the first step in screening for Wilson's disease (WD). Despite the rarity of WD in the general population, ceruloplasmin levels are routinely measured through hepatitis screening in both adults and children. Herein, we evaluated the diagnostic value of ceruloplasmin for the diagnosis of WD among children with hepatitis. Methods We retrospectively reviewed data on serum ceruloplasmin levels measured as a serologic marker for patients with hep...

  7. X-ray diffraction study of highly purified human ceruloplasmin

    International Nuclear Information System (INIS)

    The three-dimensional structure of ceruloplasmin (CP) with unoccupied labile metal-binding sites and the structure of CP containing Ni2+ in the labile sites were solved for the first time at 2.6 and 2.95 A resolution, respectively. Crystallization was performed with the use of storage-stable CP, which was prepared in the presence of proteinase inhibitors and purified from (pre)proteinases. Ceruloplasmin with Ni2+ crystallized in the orthorhombic space group, which had been earlier unknown for CP. Ceruloplasmin with the unoccupied labile sites crystallized in the trigonal crystal form. The differences in intermolecular contacts observed in the trigonal and orthorhombic crystal structures of CP are considered. The conformational changes attendant upon Ni2+ binding are described. It was suggested that the labile sites are multifunctional and can both bind metal ions potentially toxic to organisms and be involved in electron transfer from substrates to the active site.

  8. Ceruloplasmin-ferroportin system of iron traffic in vertebrates

    Institute of Scientific and Technical Information of China (English)

    Giovanni; Musci; Fabio; Polticelli; Maria; Carmela; Bonaccorsi; di; Patti

    2014-01-01

    Safe trafficking of iron across the cell membrane is a delicate process that requires specific protein carriers. While many proteins involved in iron uptake by cells are known, only one cellular iron export protein has been identified in mammals: ferroportin(SLC40A1). Ceruloplasmin is a multicopper enzyme endowed with ferroxidase activity that is found as a soluble isoform in plasma or as a membrane-associated isoform in specific cell types. According to the currently accepted view, ferrous iron transported out of the cell by ferroportin would be safely oxidized by ceruloplasmin to facilitate loading on transferrin. Therefore, the ceruloplasminferroportin system represents the main pathway for cellular iron egress and it is responsible for physiological regulation of cellular iron levels. The most recent findings regarding the structural and functional features of ceruloplasmin and ferroportin and their relationship will be described in this review.

  9. Human ceruloplasmin. Intramolecular electron transfer kinetics and equilibration

    DEFF Research Database (Denmark)

    Farver, O; Bendahl, L; Skov, L K;

    1999-01-01

    Pulse radiolytic reduction of disulfide bridges in ceruloplasmin yielding RSSR(-) radicals induces a cascade of intramolecular electron transfer (ET) processes. Based on the three-dimensional structure of ceruloplasmin identification of individual kinetically active disulfide groups and type 1 (T1...... with a rate constant of 3.9 +/- 0.8. No reoxidation of T1B Cu(I) could be resolved. It appears that the third T1 center (T1C of domain 2) is not participating in intramolecular ET, as it seems to be in a reduced state in the resting enzyme....

  10. CA-125 and Ceruloplasmin Levels in Ovarian Cancer Patients

    Directory of Open Access Journals (Sweden)

    Mangala Hegde

    2015-09-01

    Conclusion: Serum ceruloplasmin as well CA-125 level decline after treatment, and have been associated with efficacy and safety of novel therapeutic strategy to improve diagnosis and treatment for cancer. [Cukurova Med J 2015; 40(3.000: 510-516

  11. Serum Selenium and Ceruloplasmin in Nigerians with Peripartum Cardiomyopathy

    Directory of Open Access Journals (Sweden)

    Kamilu M. Karaye

    2015-04-01

    Full Text Available The study aimed to determine if selenium deficiency, serum ceruloplasmin and traditional birth practices are risk factors for peripartum cardiomyopathy (PPCM, in Kano, Nigeria. This is a case-control study carried out in three hospitals, and PPCM patients were followed up for six months. Critically low serum selenium concentration was defined as <70 µg/L. A total of 39 PPCM patients and 50 controls were consecutively recruited after satisfying the inclusion criteria. Mean serum selenium in patients (61.7 ± 14.9 µg/L was significantly lower than in controls (118.4 ± 45.6 µg/L (p < 0.001. The prevalence of serum selenium <70 µg/L was significantly higher among patients (76.9% than controls (22.0% (p < 0.001. The mean ceruloplasmin and prevalence of socio-economic indices, multiparity, pregnancy-induced hypertension, obesity and twin pregnancy were not different between the groups (p > 0.05. Logistic regression showed that rural residency significantly increased the odds for serum selenium <70 µg/L by 2.773-fold (p = 0.037. Baseline serum levels of selenium and ceruloplasmin were not associated with six-month mortality. This study has shown that selenium deficiency is a risk factor for PPCM in Kano, Nigeria, and is related to rural residency. However, serum ceruloplasmin, customary birth practices and some other characteristics were not associated with PPCM in the study area.

  12. Milk ceruloplasmin and its expression by mammary gland and liver in pigs.

    Science.gov (United States)

    Cerveza, P J; Mehrbod, F; Cotton, S J; Lomeli, N; Linder, M C; Fonda, E G; Wickler, S J

    2000-01-15

    Concentrations of ceruloplasmin and copper in milk and blood plasma, the nature of milk ceruloplasmin, and the effects of lactation and gestation on these parameters, as well as the expression of ceruloplasmin mRNA by the mammary gland, were examined in pigs. As seen previously in humans, ceruloplasmin and copper concentrations in sow milk were much higher a few days after birth than 1 month later, averaging 26.5 and 6.6 mg ceruloplasmin/L (by immunoassay) and 1.67 and 0.34 mg total Cu/L, on days 3 and 33 postpartum, respectively. Values for ceruloplasmin oxidase activity (measured with p-phenylene diamine) were 7.8 and 1.3 nmol/min/L, respectively. Daily milk ceruloplasmin production went from 61 to 22 mg/day and daily copper output from 38 to 12 mg/day. In contrast, there was little or no variation in serum ceruloplasmin concentration during lactation or gestation, although total plasma copper was high at the end of gestation. Milk ceruloplasmin was of the same apparent size as serum ceruloplasmin, as determined by SDS-PAGE and immunoblotting, and ceruloplasmin mRNAs of liver and mammary gland were indistinguishable by Northern analysis and RT-PCR of the various exons. Expression of total RNA and ceruloplasmin mRNA, as detected in biopsies of mammary gland, increased markedly upon onset of lactation and then declined during the next month in conjunction with a drop in milk ceruloplasmin production. The results indicate that milk ceruloplasmin, while being the same protein as in plasma, is not derived from the plasma but is produced by the mammary gland. PMID:10620372

  13. The relationship of ceruloplasmin and neural tube defects

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    Çağlar Yazıcıoğlu

    2010-06-01

    Full Text Available Objective: To compare the levels of ceruloplasmin (cp in the amniotic fluids and maternal bloods of second trimester fetuses with and without neural tube defects (NTD.Materials and Methods: 66 pregnant women were included in the study. Amniocentesis was performed in 32 women in a patient group diagnosed as NTD or anencephaly and 34 pregnants in a control group with positive Down Syndrome screening test. Maternal bloods were also taken. Cp was measured with Erel’s ceruloplasmin measurement method.Results: The cp levels of the amniotic fluid of patients and controls were not statistically different (p>0.05. The cp levels of the maternal bloods were not different in two groups (p>0.05.Conclusion: As an antioxidant, no relation was found between cp and NTD.

  14. Effect of estrogen on plasma ceruloplasmin level in rats exposed to acute stress

    OpenAIRE

    Ganaraja B; Pavithran P; Ghosh S

    2004-01-01

    BACKGROUND: Plasma ceruloplasmin, a copper containing protein, belongs to a class called acute phase proteins. Reduced level of ceruloplasmin was associated with Wilson′s disease and Menke′s kinky hair disease in man, primarily affecting copper metabolism. Stress was known to increase Ceruloplasmin. Several stress associated changes were commonly observed in women at menopause and also those who underwent overiectomy. Present experiment investigated the effect of estrogen on cer...

  15. Molecular responses of ceruloplasmin to Edwardsiella ictaluri infection and iron overload in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Ceruloplasmin is a serum ferroxidase that carries more than 90% of the copper in plasma and has documented roles in iron homeostasis as well as antioxidative functions. In our previous studies, it has been shown that the ceruloplasmin gene is strongly up-regulated in catfish during challenge with Ed...

  16. Effect of estrogen on plasma ceruloplasmin level in rats exposed to acute stress

    Directory of Open Access Journals (Sweden)

    Ganaraja B

    2004-04-01

    Full Text Available BACKGROUND: Plasma ceruloplasmin, a copper containing protein, belongs to a class called acute phase proteins. Reduced level of ceruloplasmin was associated with Wilson′s disease and Menke′s kinky hair disease in man, primarily affecting copper metabolism. Stress was known to increase Ceruloplasmin. Several stress associated changes were commonly observed in women at menopause and also those who underwent overiectomy. Present experiment investigated the effect of estrogen on ceruloplasmin level in acute stress. AIMS: To assess the estradiol induced changes in plasma ceruloplasmin concentration on exposure of the rats to acute stress. SETTINGS AND DESIGN: Acute stress was induced by forcing the rats to swim till exhaustion. The rats were overiectomised bilaterally to remove the primary source of sex hormones. And hormone replacement was done later. MATERIAL AND METHODS: Wistar albino female rats were used. Acute stress was induced before overiectomy, following recovery from surgery, and again after Estradiol Valerate injection (for 10 days in same group of rats. The plasma ceruloplasmin was estimated immediately after stress during each stage - that is preoperative control, stressed control, after overiectomy and then following treatment with Estradiol Valerate. STATISTICAL ANALYSIS USED: Paired sample T test was applied to analyze the findings. Results: We found lowest ceruloplasmin level after stress in overiectomised animals, while on substitution of estradiol the trend appeared to be reversed. CONCLUSION: The result suggested a direct effect of estrogen on hepatic ceruloplasmin production/release and this could account for some of the beneficial effects of hormone replacement therapy.

  17. Ceruloplasmin Oxidation, a Feature of Parkinson's Disease CSF, Inhibits Ferroxidase Activity and Promotes Cellular Iron Retention

    KAUST Repository

    Olivieri, S.

    2011-12-14

    Parkinson\\'s disease is a neurodegenerative disorder characterized by oxidative stress and CNS iron deposition. Ceruloplasmin is an extracellular ferroxidase that regulates cellular iron loading and export, and hence protects tissues from oxidative damage. Using two-dimensional electrophoresis, we investigated ceruloplasmin patterns in the CSF of human Parkinson\\'s disease patients. Parkinson\\'s disease ceruloplasmin profiles proved more acidic than those found in healthy controls and in other human neurological diseases (peripheral neuropathies, amyotrophic lateral sclerosis, and Alzheimer\\'s disease); degrees of acidity correlated with patients\\' pathological grading. Applying an unsupervised pattern recognition procedure to the two-dimensional electrophoresis images, we identified representative pathological clusters. In vitro oxidation of CSF in two-dimensional electrophoresis generated a ceruloplasmin shift resembling that observed in Parkinson\\'s disease and co-occurred with an increase in protein carbonylation. Likewise, increased protein carbonylation was observed in Parkinson\\'s disease CSF, and the same modification was directly identified in these samples on ceruloplasmin. These results indicate that ceruloplasmin oxidation contributes to pattern modification in Parkinson\\'s disease. From the functional point of view, ceruloplasmin oxidation caused a decrease in ferroxidase activity, which in turn promotes intracellular iron retention in neuronal cell lines as well as in primary neurons, which are more sensitive to iron accumulation. Accordingly, the presence of oxidized ceruloplasmin in Parkinson\\'s disease CSF might be used as a marker for oxidative damage and might provide new insights into the underlying pathological mechanisms.

  18. Lipoperoxides, alpha-tocopherol and ceruloplasmin in gamma-irradiated blood plasma

    International Nuclear Information System (INIS)

    Ceruloplasmin, alpha-tocopherol and lipid peroxide concentrations are evaluated in blood plasma for transfusion following exposure to irradiation with 60Co gamma rays at doses 23, 50, 100 and 200 Gy. In plasma exposed to irradiation an increase in lipid peroxides and decrease in alpha-tocopherol and ceruloplasmin are observed. The addition of 2.3 U/ml ceruloplasmin to plasma prior to irradiation reduces the quantity of lipid peroxides and protects alpha-tocopherol. The possible explanation is that the metal helates prevent the formation of free hydroxyl radicals and thus inhibit the oxidation of lipid membranes. 15 refs., 1 tab. (author)

  19. Isolation and characterization of a processed gene for human ceruloplasmin

    International Nuclear Information System (INIS)

    A processed pseudogene for human ceruloplasmin has been isolated that contains DNA corresponding to the functional gene sequence encoding the carboxy-terminal 563 amino acid residues and the 3' untranslated region. The pseudogene appears to have arisen from a processed RNA species, since intervening sequences coincident with those of the functional gene have been removed, with the exception of a short segment of intronic sequence which denotes the 5' boundary of the pseudogene. The nucleotide sequence of the pseudogene is highly homologous (97% sequence identity) with that of the wild-type gene, suggesting that pseudogene formation was a relatively recent evolutionary event. In addition to single base substitutions, there is a large 213 base pair (bp) deletion in the pseudogene sequence which corresponds to the location of an intron-exon junction in the functional gene. A 4 bp duplication that occurs at amino acid residue 683 of the wild-type coding sequence results in a frameshift mutation and introduces a premature translational termination codon at this point. This is concordant with the inability to detect a human liver transcript corresponding to the pseudogene by nuclease S1 mapping analysis. The 3' end of the pseudogene is characterized by a 62 bp segment composed mainly of repeated TC dinucleotides. On the basis of genomic Southern blot analysis performed under high-stringency conditions, the pseudogene that the authors have identified seems to comprise the only sequence in the human genome that is closely related to the wild-type gene. Using somatic cell hybridization, they have mapped the pseudogene to human chromosome 8. This differs from the site of the wild-type ceruloplasmin locus, which has been assigned to chromosome 3

  20. Role of serum copper and ceruloplasmin level in patients with dysfunctional uterine bleeding

    Directory of Open Access Journals (Sweden)

    Ketki P. Khandhadiya

    2014-04-01

    Conclusions: This study has concluded that serum copper and ceruloplasmin could be a sensitive indicator of angiogenesis in endometrium leading to DUB in females. [Int J Reprod Contracept Obstet Gynecol 2014; 3(2.000: 330-334

  1. Ceruloplasmin Alters the Tissue Disposition and Neurotoxicity of Manganese, but not its Loading onto Transferrin

    OpenAIRE

    Jursa, Thomas; Smith, Donald R.

    2008-01-01

    Manganese (Mn) is a redox-active element, and whereas its uptake, disposition, and toxicity in mammals may depend in part on its oxidation state, the proteins affecting manganese oxidation state and speciation in vivo are not well known. Studies have suggested that the oxidase protein ceruloplasmin (Cp) mediates iron and manganese oxidation and loading onto plasma transferrin (Tf), as well as cellular iron efflux. We hypothesized that ceruloplasmin may also affect the tissue distribution and ...

  2. Inhibition of cell mediated immune responses by copper, ceruloplasmin and oral contraceptives

    International Nuclear Information System (INIS)

    We have shown that free copper inhibited lymphocyte transformation in the whole blood lymphocyte cultures. Ceruloplasmin also inhibited lymphocyte transformation in the above tests as well as another test involving purified protein derivative from Mycobacterium tuberculosis. Women on oral contraceptives have elevated serum copper and ceruloplasmin levels, as do cancer patients. We hypothesize that women on oral contraceptives may have decreased cell mediated immune responses and may thus be at higher risk for cancer induction

  3. Immune mechanisms of the radioprotective effects of ceruloplasmin

    International Nuclear Information System (INIS)

    Immunotropic features of ceruloplasmin (Cp) were examined and its capacity as radioprotector was estimated. Pulse radiolysis method was used to set the mechanism of elementary reactions, which were responsible for antioxidant activity and to demonstrate a particular role of reversible oxycomplexes Cp...O2. Within in vitro test-systems the effects of Cp were examined on the model of interaction between lymphocytes from Shelter staff which constantly contacts with ionizing radiation and autologous erythrocytes in rosset-forming phenomenon. Expression of this Index in the presence of antigens with known polyspecificity was determined (antigens were taken from cortical and pyramid sections of kidney, liver, lungs, myocard, pancreatic gland, grey matter and aorta). Simultaneously the presence of analogous autoantibodies was determined. It has been shown that Cp can reprogram the level of expression of immunovaluable receptors towards to norm. It has been also defined that Cp presence in blood irradiated in vitro (1-15 rem) promotes the masking of active centers of autoantibodies of different tissue specificity

  4. Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis

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    Rajesha Rupaimoole

    2015-12-01

    Full Text Available Long noncoding RNAs (lncRNAs significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer.

  5. The molecular mechanism for interaction of ceruloplasmin and myeloperoxidase

    Science.gov (United States)

    Bakhautdin, Bakytzhan; Bakhautdin, Esen Göksöy

    2016-04-01

    Ceruloplasmin (Cp) is a copper-containing ferroxidase with potent antioxidant activity. Cp is expressed by hepatocytes and activated macrophages and has been known as physiologic inhibitor of myeloperoxidase (MPO). Enzymatic activity of MPO produces anti-microbial agents and strong prooxidants such as hypochlorous acid and has a potential to damage host tissue at the sites of inflammation and infection. Thus Cp-MPO interaction and inhibition of MPO has previously been suggested as an important control mechanism of excessive MPO activity. Our aim in this study was to identify minimal Cp domain or peptide that interacts with MPO. We first confirmed Cp-MPO interaction by ELISA and surface plasmon resonance (SPR). SPR analysis of the interaction yielded 30 nM affinity between Cp and MPO. We then designed and synthesized 87 overlapping peptides spanning the entire amino acid sequence of Cp. Each of the peptides was tested whether it binds to MPO by direct binding ELISA. Two of the 87 peptides, P18 and P76 strongly interacted with MPO. Amino acid sequence analysis of identified peptides revealed high sequence and structural homology between them. Further structural analysis of Cp's crystal structure by PyMOL software unfolded that both peptides represent surface-exposed sites of Cp and face nearly the same direction. To confirm our finding we raised anti-P18 antisera in rabbit and demonstrated that this antisera disrupts Cp-MPO binding and rescues MPO activity. Collectively, our results confirm Cp-MPO interaction and identify two nearly identical sites on Cp that specifically bind MPO. We propose that inhibition of MPO by Cp requires two nearly identical sites on Cp to bind homodimeric MPO simultaneously and at an angle of at least 120 degrees, which, in turn, exerts tension on MPO and results in conformational change.

  6. Changes in Serum Zinc, Copper and Ceruloplasmin Levels of Whole Body Gamma Irradiated Rats

    International Nuclear Information System (INIS)

    Rats are whole body irradiated with different Gamma radiation doses. Zinc and Copper, two important trace elements in the biological processes and Ceruloplasmin, a protein which carries more than 95% of serum Cu and has important roles in many vital processes are followed up in the irradiated rat sera. This work aimed to determine the changes in the serum levels of the three parameters (Zinc, Copper and Ceruloplasmin) through eight weeks follow up period (1st, 2nd, 3rd, 4th, 6th, and 8th week) post whole body gamma irradiation with three sub-lethal doses (2, 3.5 and 5 Gy) of rats. All the experimental animals did not receive any medical treatment. Zinc and Copper were measured using discrete nebulization flame atomic absorption spectrometry. Ceruloplasmin was measured using a colorimetric method. The statistical analyses of the results show that the Zinc levels of the irradiated groups decreased significantly post irradiation and then were recovered at the 6th week post irradiation. The Copper levels of the irradiated groups increased significantly and then were recovered at 6th week post irradiation. The levels of Ceruloplasmin in the same groups increased significantly throughout the whole follow up period. The conclusion is that, Zinc, Copper and Ceruloplasmin levels changed significantly in the irradiated groups compared to the control group with a maximum effect noted in the groups irradiated with the higher doses and that the lower dose irradiated groups recover earlier than the higher ones. Also the correlation between Copper and Zinc is reversible at different doses and that between Copper and Ceruloplasmin is direct

  7. Mechanism of Copper Uptake from Blood Plasma Ceruloplasmin by Mammalian Cells.

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    Danny Ramos

    Full Text Available Ceruloplasmin, the main copper binding protein in blood plasma, has been of particular interest for its role in efflux of iron from cells, but has additional functions. Here we tested the hypothesis that it releases its copper for cell uptake by interacting with a cell surface reductase and transporters, producing apoceruloplasmin. Uptake and transepithelial transport of copper from ceruloplasmin was demonstrated with mammary epithelial cell monolayers (PMC42 with tight junctions grown in bicameral chambers, and purified human 64Cu-labeled ceruloplasmin secreted by HepG2 cells. Monolayers took up virtually all the 64Cu over 16h and secreted half into the apical (milk fluid. This was partly inhibited by Ag(I. The 64Cu in ceruloplasmin purified from plasma of 64Cu-injected mice accumulated linearly in mouse embryonic fibroblasts (MEFs over 3-6h. Rates were somewhat higher in Ctr1+/+ versus Ctr1-/- cells, and 3-fold lower at 2°C. The ceruloplasmin-derived 64Cu could not be removed by extensive washing or trypsin treatment, and most was recovered in the cytosol. Actual cell copper (determined by furnace atomic absorption increased markedly upon 24h exposure to holoceruloplasmin. This was accompanied by a conversion of holo to apoceruloplasmin in the culture medium and did not occur during incubation in the absence of cells. Four different endocytosis inhibitors failed to prevent 64Cu uptake from ceruloplasmin. High concentrations of non-radioactive Cu(II- or Fe(III-NTA (substrates for cell surface reductases, or Cu(I-NTA (to compete for transporter uptake almost eliminated uptake of 64Cu from ceruloplasmin. MEFs had cell surface reductase activity and expressed Steap 2 (but not Steaps 3 and 4 or dCytB. However, six-day siRNA treatment was insufficient to reduce activity or uptake. We conclude that ceruloplasmin is a circulating copper transport protein that may interact with Steap2 on the cell surface, forming apoceruloplasmin, and Cu(I that enters

  8. [Ceruloplasmin and peptides of medium molecular weight as parameters in development of acute myeloblastic leukemia].

    Science.gov (United States)

    Kovtunova, M E; Pan'kov, V N; Perevalova, N N

    2003-05-01

    A dynamic content of ceruloplasmin and medium-molecular peptides were studied in the blood serum of 28 patients with an acute clinical course of myeloblast-type leucosis during a scheduled chemotherapy. The mentioned biochemical criteria were shown to be able to characterize indirectly an intensity of the clinical course, functional liver condition and the efficiency of chemotherapy. PMID:12822312

  9. Role of serum copper and ceruloplasmin level in patients with dysfunctional uterine bleeding

    OpenAIRE

    Ketki P. Khandhadiya; Yousef Rezaei Chianeh; Pragna Rao

    2014-01-01

    Background: Objective of current study was to study serum copper and ceruloplasmin levels in abnormal endometrial angiogenesis observed in dysfunctional uterine bleeding patients. Methods: Design: The present cross sectional study was undertaken in the departments of biochemistry and department of OBG, Kasturba medical college, Manipal University, Manipal, India. Population: This study was done in 40 females age between 18-45 years with history of, bleeding excessively for more than 3 mont...

  10. Plasma Glucose and Serum Ceruloplasmin in Metabolic Syndrome and Diabetes Mellitus Type 2

    OpenAIRE

    Ashok Kumar Jeppu; Asha Augusthy; Kavitha Ashok Kumar

    2016-01-01

    Diabetes mellitus type 2 and metabolic syndrome are conditions associated with insulin resistance and hyperglycemia. Metabolic syndrome is a risk factor for diabetes mellitus type 2. Plasma glucose (fasting/postprandial) and serum ceruloplasmin levels and their relationship were studied. Study population consisted of 150 individuals—50 individuals with diabetes mellitus type 2, 50 individuals with metabolic syndrome, and 50 age- and sex-matched healthy controls. Plasma levels of fasting and p...

  11. Association of bilirubin and protein thiols in relation to copper and ceruloplasmin in hyperbilirubinemic patients

    Institute of Scientific and Technical Information of China (English)

    Mungli Prakash; Jeevan K Shetty; Roshan D'Souza; Suhasa Upadhya; Vijay Kumar

    2009-01-01

    Objective:Bilirubin is a double edged sword in biological system,acting as a toxic molecule and cytoprotecrant.Unconjugated bilirubin is proved to show antioxidant activity in vitro and in vivo.In the current work we tried to know the relationship between both conjugated and unconjugated bilirubin with copper and protein thiols in patients with hyperbilirnbinemia.Methods:Study was conducted on 56 hyperbilirubinemic cases and 56 healthy controls.Serum copper,ceruloplasmin,protein thiols,total bilirubin,conjugated and unconjugated bilirubin,unconjugated bilimbin/albumin ratio,total protein,albumin,AST,ALT and ALP were estimated.Results:There was significant increase in serum copper,total bilirubin,conjugated and unconjugated bilimbin.uriconjugated bilirubin/albumin ratio,AST,ALT,and ALP,and decrease in serum ceruloplasmin,protein thiols,total protein,and albumin in hyperbilimbinemic cases when compared to healthy controls.Conjugated bilimbin correlated positively with liver enzymes AST and ALP,and negatively with protein thials,total protein and albumin.Unconjugated bilirubin correlated positively with ALT.Protein thiols correlated negatively with copper and positively with ceruloplasmin,and also correlated negativelv with liver enzymes like AST,ALT and ALP,and positively with total protein and albumin.Conclusion:Combination of elevated levels of trace elements like copper and availability of reducing agent like bilimbin may prove deleterious by generating free radicals.

  12. A STUDY ON RELATIONSHIP BETWEEN FASTING PLASMA GLUCOSE, COPPER AND CERULOPLASMIN LEVELS IN TYPE 2 DIABETES MELLITUS

    Directory of Open Access Journals (Sweden)

    Rangaswamy

    2015-08-01

    Full Text Available INTRODUCTION: Diabetes mellitus (DM is an endocrine disease associated with hyperglycemia characterized by both insulin resistance and defective insulin secretion. Copper a transition metal is present in many tissues like liver, muscle etc., It can oxidize proteins and lipids which lead to increased production of free radical compounds. Ceruloplasmin an alpha 2 globulin is an acute phase copper containing plasma protein synthesized mainly by hepatic parenchymal cells, lymphocytes etc., Reactive oxygen species (ROS/ free radicals production due to hyperglycemia in diabetes mellitus can directly or indirectly alter the integrity and physiological function of cells. Present study was undertaken to study the relationship b etween fasting plasma glucose (FPG, copper and ceruloplasmin in type 2 Diabetes mellitus. MATERIALS AND METHODOLOGY: The study group consisted of a total 100 subjects which included non - diabetic healthy control subjects (n = 50 and type 2 diabetic patien ts (n = 50. Fasting blood samples were collected and analysed for estimation of fasting plasma glucose, serum copper and ceruloplasmin. RESULTS : Results shows increase in levels of copper and FPG (P<0.001 and decrease in ceruloplasmin (P<0.001 in type 2 DM patients compared to healthy controls. CONCLUSION: Our study shows an increase in copper and FPG with decreased levels of ceruloplasmin which may be due to generation of ROS which leads to increased consumption of available antioxidants in the body .

  13. Ceruloplasmin reduces DNA double strand breaks and improves cell survival in lymphoblastoid cells

    International Nuclear Information System (INIS)

    Full text: Ionizing radiation through oxidative free radical production causes dose-dependent oxidative damage to biological macromolecules. To reduce the oxidative stress from ionizing radiation, use of antioxidants has been suggested as a prophylactic and early remedy of pathogenic therapy. Ceruloplasmin (Cp), a plasma protein produced by the liver, belongs to a class of multi copper ferroxidases known for their role in iron metabolism in vertebrates, including humans. Functions of Cp include copper transport, ferroxidase and aminooxidase activities. Serum Cp concentration fluctuates during inflammation, infection, trauma and irradiation. The role of Cp as an antioxidant after irradiation is not fully understood. Our aim was to investigate the radioprotective efficacy of Cp. We studied the effect of ceruloplasmin on the in-vitro radiosensitivity of lymphoblastoid cells lines after gamma-ray irradiation. We used radiosensitive cell lines LB0003, LB0004 and LB 0005, established from individuals who developed late radiation necrosis following curative radiotherapy and non-sensitive cell lines Masci and LB0001 as controls. The cell lines were irradiated with doses from 0 - 60 Gy. Genomic DNA was extracted at 0 - 24h after irradiation and subjected to PFGE to analyse the initial quantity of DNA DSBs and the quantity of unrepaired DSBs to evaluate the kinetics of DSB rejoining. Human Cp (0.05 or 0.5mg/ml) was added to cell cultures 30 min before or 5 min after irradiation. In the presence of Cp cell survival was increased and the level of DBSs reduced demonstrating its radioprotective effect and the potential mechanism of its protection against radiation effects. Its radioprotective efficacy on dose and time of administration of Cp. The radiosensitive cell lines differ from controls by kinetics of DSBs repair. Importantly, in presence of ceruloplasmin the level of DNA DSB was reduced and the kinetics of DNA DSB repair became comparable to that in controls

  14. Determination of Serum Ceruloplasmin Concentration in Patients with Primary Open Angle Glaucoma with Cataract and Patients with Cataract Only: A Pilot Study

    Science.gov (United States)

    Sarnat-Kucharczyk, Monika; Rokicki, Wojciech; Zalejska-Fiolka, Jolanta; Pojda-Wilczek, Dorota; Mrukwa-Kominek, Ewa

    2016-01-01

    Background The aim of this article was to describe the role of ceruloplasmin and to report preliminary results of ceruloplasmin concentrations in patients with primary open-angle glaucoma (POAG) with cataract and in patients with only cataract. Glaucoma, a neurodegenerative disease, is a heterogeneous group of conditions characterized by loss of retinal ganglion cells (RGC), their axons, progressive optic nerve damage, and visual field deterioration. Material/Methods The POAG group included 30 patients and the cataract group included 25 patients. Results Ceruloplasmin plays an essential role in iron metabolism and inactivating free radicals. In the presented pilot study, serum ceruloplasmin level was lower in the POAG group in comparison to the group with only cataract. Conclusions In treating persistent inflammation in the course of glaucoma, antiglaucoma drugs may increase the permeability of the blood-ocular barrier, which may be connected with the lower concentration of serum ceruloplasmin in the glaucoma patients group. PMID:27109647

  15. Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites

    International Nuclear Information System (INIS)

    The three-dimensional molecular structure of human serum ceruloplasmin has been reinvestigated using X-ray synchrotron data collected at 100 K from a crystal frozen to liquid-nitrogen temperature. The three-dimensional molecular structure of human serum ceruloplasmin has been reinvestigated using X-ray synchrotron data collected at 100 K from a crystal frozen to liquid-nitrogen temperature. The resulting model, with an increase in resolution from 3.1 to 2.8 Å, gives an overall improvement of the molecular structure, in particular the side chains. In addition, it enables the clear definition of previously unidentified Ca2+-binding and Na+-binding sites. The Ca2+ cation is located in domain 1 in a configuration very similar to that found in the activated bovine factor Va. The Na+ sites appear to play a structural role in providing rigidity to the three protuberances on the top surface of the molecule. These features probably help to steer substrates towards the mononuclear copper sites prior to their oxidation and to restrict the size of the approaching substrate. The trinuclear copper centre appears to differ from the room-temperature structure in that a dioxygen moiety is bound in a similar way to that found in the endospore coat protein CotA from Bacillus subtilis

  16. Isolation and characterization of ceruloplasmin-like protein from, serum of a patient with Wilson-Konovalov disease

    International Nuclear Information System (INIS)

    Results are presented of comparitive investigation into ceruloplasmin (CP) isolated by the affined chromatography method from the serum of a healthy person and a patient with the Wilson-Konovalov disease. In this case violation of the CP gene expression is mnifested in synthesis and secretion of anomalous CP. This CP differs from the normal CP in physicochemical, immunological and catalytic properties

  17. Role of liver endothelium in the binding and uptake of ceruloplasmin: studies with colloidal gold probe

    International Nuclear Information System (INIS)

    To determine the mode of uptake of ceruloplasmin (CP) by liver, the protein was labeled with colloidal gold and infused into the portal vein. In cold almost all probes bound to the sinusoidal endothelium, and at 370C internalization via a system of coated pits and vesicles occurred. Only rarely did the probe appear to bypass the endothelium, moving to the albuminal side through the gaps between endothelial cells. In the endothelial cytoplasm, the probe was seen in coated vesicles, endosomes, tubules, and large vesicles which may have formed by fusion of endosomes and tubules. Moreover, externalization of the probe to the abluminal side was noted, and this also occurred via a system of coated vesicles. The findings suggest that the uptake of CP in the liver may be primarily a transendothelial phenomenon (transcytosis)

  18. Induction of ceruloplasmin synthesis by interleukin-1 in copper deficient and copper sufficient rats

    International Nuclear Information System (INIS)

    Ceruloplasmin (Cp) is a copper-containing plasma protein important in the body's acute phase defense system. In copper sufficient rats given two injections of interleukin-1 (IL-1) at 0 and 8 h, ceruloplasmin activity began to significantly increase within 6 h, but did not peak until at least 24 h. The 24 h stimulated activity was 84 +/- 2 umole p-phenylene diamine (pPD) oxidized x min-1 x L-1 compared to a control of 43 +/- 5. These rats were injected with 100uCi 3H-leucine (ip) 2 h before sacrifice to label newly synthesized proteins. When the 3H immunoprecipitated by rabbit anti-rat Cp serum is expressed as a percent of the 3H precipitated by trichloroacetic acid (TCA), the basal Cp synthesis rate was 3% of the total serum protein synthesis. The rate of Cp synthesis peaked 12 h after IL-1 injection at 7% of total serum protein synthesis and by 24 h was back to the basal rate. In copper deficient rats, IL-1 given with copper induced pPD oxidase activity, while IL-1 given alone did not stimulate activity. The basal Cp synthesis rate in these rats was 3%, the same as in the copper sufficient rats. In copper deficient rats, the Cp synthesis rate was induced by IL-1 with or without an injection of copper. Therefore, if dietary copper is in short supply, then although Cp synthesis is induced by this mediator of host defense mechanisms, Cp cannot carry out its functions

  19. Fibrinogen and ceruloplasmin in plasma and milk from dairy cows with subclinical and clinical mastitis.

    Science.gov (United States)

    Tabrizi, A Davasaz; Batavani, R A; Rezaei, S Asri; Ahmadi, M

    2008-02-15

    The potential using of Acute Phase Proteins (APPs) in the assessment of mammary gland health was studied by examining the levels of Fibrinogen (Fb) and Ceruloplasmin (Cp) in plasma and milk from dairy cows with different grades of mastitis. Plasma samples were taken from jugular vein and milk samples were collected from quarters of cows with subclinical and clinical mastitis, as well as healthy controls. California Mastitis Test (CMT) were performed on each udder quarter of cows for detection of CMT2+ and CMT3+ quarters. CMT (0) and culture negative cases were considered healthy cows. Clinical mastitis, was graded as mild (clots in milk) or moderate (clots in milk and visible signs of inflammation in the mammary gland/s). The concentrations of Fb in the plasma of the cows with subclinical and clinical mastitis were higher than in the plasma of the healthy cows (p0.05), but differences between clinical and healthy groups were significant (pmastitis were higher than in the milk of the healthy cows (pmastitis in dairy cows. PMID:18817128

  20. Serum Levels of Copper, Ceruloplasmin and Angiotensin Converting Enzyme among Silicotic and Non-Silicotic Workers

    Science.gov (United States)

    Beshir, Safia; Aziz, Hisham; Shaheen, Weam; Eltahlawy, Eman

    2015-01-01

    BACKGROUND: Silicosis is the most frequently occurring pneumoconiosis. AIM: Measurement of serum levels of Angiotensin converting enzyme (ACE), Copper (Cu) and Ceruloplasmin (Cp) in cement workers occupationally exposed to silica dust as biomarkers of exposure rather than biomarkers of effect for silicosis. METHODS: Plain chest X-ray & pulmonary functions were done for 30 silicotic and 42 non-silicotic workers and 42 controls. CT scan was done for the exposed groups. Serum levels of Cu, Cp and ACE were estimated. RESULTS: The results showed a higher significant difference between the exposed groups and controls, and between the two exposed groups regarding the mean levels of all measured biochemical parameters. The pulmonary functions were significantly lower among silicotic workers than controls and non-silicotic groups. There was a significant positive correlation between duration of employment and serum ACE and Cu. CONCLUSION: Since respirable dust exposure-linked lung fibrosis disease is non-curable, the biochemical parameters (Cu, ACE and Cp) can be used as exposure biomarkers to silica dust, providing a better way for early diagnosis of this deadly disease. Down regulating the inflammatory responses could potentially reduce the adverse clinical pulmonary effects of air pollution.

  1. Copper balance and ceruloplasmin in chronic hepatitis in a Wilson disease animal model, LEC rats

    Energy Technology Data Exchange (ETDEWEB)

    Komatsu, Yutaka; Ogra, Yasumitsu; Suzuki, Kazuo T. [Graduate School of Pharmaceutical Sciences, Chiba University, Inage, Chiba 263-8522 (Japan)

    2002-09-01

    In an animal model of Wilson disease, Long-Evans rats with cinnamon-colored coat (LEC rats), copper (Cu) accumulates in the liver with age up to the onset of acute hepatitis owing to a hereditary defective transporter for the efflux of Cu, ATP7B. The plasma Cu concentration is low in LEC rats because of the excretion of apo-ceruloplasmin (apo-Cp). However, toward and after the onset of chronic hepatitis, plasma Cu concentration increases in the form of holo-Cp, while the liver Cu concentration is maintained at a constant level without the occurrence of fulminant hepatitis. In the present study, the material balance of Cu was studied in LEC rats with chronic hepatitis in order to elucidate the mechanisms underlying the increase of holo-Cp in plasma and the maintenance of Cu at a constant level in the liver. The relationship between the Cu concentration and ferroxidase activity of Cp was analyzed in the plasma of LEC rats of different ages and of Wistar rats fed a Cu-deficient diet for different durations. Cu was suggested to be delivered to Cp in an all-or-nothing manner, resulting in the excretion of fully Cu-occupied holo-Cp (Cu{sub 6}-Cp) or totally Cu-unoccupied Cu{sub 0}-Cp (apo-Cp), but not partially Cu-occupied Cu{sub n}-Cp (where n=1-5). The increase of holo-Cp in acute and chronic hepatitis in LEC rats was explained by the delivery of Cu, accumulating in the non-metallothionein-bound form, to Cp outside the Golgi apparatus of the liver. The plasma Cu concentration and ferroxidase activity were proposed to be specific indicators of the appearance of non-metallothionein-bound Cu in the liver of LEC rats. (orig.)

  2. Dynamics of ceruloplasmin amd of some blood parameters in piglets suffering from acute radiation disease

    International Nuclear Information System (INIS)

    Changes in oxidase activity of ceruloplasmin (CP) were followed up after treatment with gamma rays of 60Co with a dose of 4 Ey (dose power 0.68 Ey/min) in the blood serum of male piglets of an average life weight of 18+-3 kg. CP level from 217 E/l raised to 376 E/l on the 16th hour after radiation and on the 16th day decreased to 296 e/l. Moreover, serum copper increased from 25 μmol/l to 42.3 μmol/l on the 16th hour following irradiation but on the 14th day its level dropped to 32.5 μmol/l. Erythrocyte and leucocyte count decreased from 5.83x1012/l and 19.94x1012/l to 2.47x1012/l and 2.0x109/l, respectively. Besides, serum albumin decreased, but α-and β-globulins increased. Examination of ailing piglets showed a paraclinical pattern of an inflammatory process by acute radiation disease. CP as an antioxidant participates in reactions with toxic radiacals on radiation. By the rising of its level on radiation it again was characterized as an acute-phase protein. Serum CP was determined according to the method of Ravin using as substrate P-phenylendiamine dihydrochloride; serum copper - by the test of Boehringer Mannheim; serum proteins were studied electrophoretically on agarose gels using veronal buffer, pH 8.6

  3. Variations in plasma ceruloplasmin and whole body retention of sup 67 Cu in guinea pigs recovering from vitamin C deficiency

    Energy Technology Data Exchange (ETDEWEB)

    Hosestenbach, R.D. Jr.; Harris, E.D. (Texas A and M Univ., College Station (United States))

    1991-03-15

    Parallels may be drawn between the symptoms of scurvy and copper deficiency. This realization led the authors to examine the effects of ascorbic acid supplementation on plasma ceruloplasmin and whole body turnover of copper in scorbutic guinea pigs. Weanling guinea pigs were fed an ascorbate free semi-purified diet for 10-14 days then randomly divided into 3 treatment groups receiving oral supplementation of ascorbic acid at levels: deficient, normal, and excess. In two experiments with different groups of animals, the plasma ceruloplasmin IU, measured by p-phenylenediamine oxidase activity, was significantly higher in the deficient groups, 53.5 {plus minus} 7.7 and 41.2 {plus minus} 9.2, than in the normal and excess groups, 18.3 {plus minus} 7.7, 21.6 {plus minus} 2.6 and 30.2 {plus minus} 9.2, 18.3 {plus minus} 2.6, respectively. {sup 67}Cu was administered intraperitoneally and whole body gamma radiation was measured at 24 h intervals to determine excretion and retention rates of the 3 treatment groups. A higher retention of {sup 67}Cu was observed in the deficient group, t1/2 = 4.8 days compared to 2.6 and 1.6 in the normal and excess groups, respectively. The affect of ascorbic acid on the regulatory mechanism of copper retention, either directly or indirectly, and the increase in plasma ceruloplasmin activity indicates ascorbic acid may perform a functional role in copper utilization in a biological system.

  4. Plasma protein thiols, ceruloplasmin, C-reactive protein and red blood cell acetylcholinesterase in patients undergoing intrauterine insemination

    OpenAIRE

    Krishnananda Prabhu; Pratap Kumar; Satish Kumar Adiga; Anjali Rao; Anupama Lanka; Jaipal Singh

    2009-01-01

    Objective: To estimate acetylcholinesterase (AChE), protein thiols (PT), ceruloplasmin (CP) and C-reactive proteins (CRPs) to assess any change in their levels following intrauterine insemination (IUI). Materials and Methods: Forty-two patients aged 31 ± 4.65 years (mean ± SD) with primary infertility selected for IUI. All of them had induced ovulation with clomiphene citrate 50 mg from day 2 to day 6. After taking the consent, 2 ml of blood was withdrawn before and after 24 h of IUI for bio...

  5. Mutations in SLC33A1 cause a lethal autosomal-recessive disorder with congenital cataracts, hearing loss, and low serum copper and ceruloplasmin

    DEFF Research Database (Denmark)

    Huppke, Peter; Brendel, Cornelia; Kalscheuer, Vera;

    2012-01-01

    compound heterozygous mutations for all affected subjects in SLC33A1 encoding a highly conserved acetylCoA transporter (AT-1) required for acetylation of multiple gangliosides and glycoproteins. The mutations were found to cause reduced or absent AT-1 expression and abnormal intracellular localization of...... the protein. We also showed that AT-1 knockdown in HepG2 cells leads to reduced ceruloplasmin secretion, indicating that the low copper in serum is due to reduced ceruloplasmin levels and is not a sign of copper deficiency. The severity of the phenotype implies an essential role of AT-1 in proper...

  6. Role of hypoxia-inducible factor-1 in transcriptional activation of ceruloplasmin by iron deficiency

    Science.gov (United States)

    Mukhopadhyay, C. K.; Mazumder, B.; Fox, P. L.

    2000-01-01

    A role of the copper protein ceruloplasmin (Cp) in iron metabolism is suggested by its ferroxidase activity and by the tissue iron overload in hereditary Cp deficiency patients. In addition, plasma Cp increases markedly in several conditions of anemia, e.g. iron deficiency, hemorrhage, renal failure, sickle cell disease, pregnancy, and inflammation. However, little is known about the cellular and molecular mechanism(s) involved. We have reported that iron chelators increase Cp mRNA expression and protein synthesis in human hepatocarcinoma HepG2 cells. Furthermore, we have shown that the increase in Cp mRNA is due to increased rate of transcription. We here report the results of new studies designed to elucidate the molecular mechanism underlying transcriptional activation of Cp by iron deficiency. The 5'-flanking region of the Cp gene was cloned from a human genomic library. A 4774-base pair segment of the Cp promoter/enhancer driving a luciferase reporter was transfected into HepG2 or Hep3B cells. Iron deficiency or hypoxia increased luciferase activity by 5-10-fold compared with untreated cells. Examination of the sequence showed three pairs of consensus hypoxia-responsive elements (HREs). Deletion and mutation analysis showed that a single HRE was necessary and sufficient for gene activation. The involvement of hypoxia-inducible factor-1 (HIF-1) was shown by gel-shift and supershift experiments that showed HIF-1alpha and HIF-1beta binding to a radiolabeled oligonucleotide containing the Cp promoter HRE. Furthermore, iron deficiency (and hypoxia) did not activate Cp gene expression in Hepa c4 hepatoma cells deficient in HIF-1beta, as shown functionally by the inactivity of a transfected Cp promoter-luciferase construct and by the failure of HIF-1 to bind the Cp HRE in nuclear extracts from these cells. These results are consistent with in vivo findings that iron deficiency increases plasma Cp and provides a molecular mechanism that may help to understand these

  7. The acute phase protein ceruloplasmin as a non-invasive marker of pseudopregnancy, pregnancy, and pregnancy loss in the giant panda.

    Directory of Open Access Journals (Sweden)

    Erin L Willis

    Full Text Available After ovulation, non-pregnant female giant pandas experience pseudopregnancy. During pseudopregnancy, non-pregnant females exhibit physiological and behavioral changes similar to pregnancy. Monitoring hormonal patterns that are usually different in pregnant mammals are not effective at determining pregnancy status in many animals that undergo pseudopregnancy, including the giant panda. Therefore, a physiological test to distinguish between pregnancy and pseudopregnancy in pandas has eluded scientists for decades. We examined other potential markers of pregnancy and found that activity of the acute phase protein ceruloplasmin increases in urine of giant pandas in response to pregnancy. Results indicate that in term pregnancies, levels of active urinary ceruloplasmin were elevated the first week of pregnancy and remain elevated until 20-24 days prior to parturition, while no increase was observed during the luteal phase in known pseudopregnancies. Active ceruloplasmin also increased during ultrasound-confirmed lost pregnancies; however, the pattern was different compared to term pregnancies, particularly during the late luteal phase. In four out of the five additional reproductive cycles included in the current study where females were bred but no birth occurred, active ceruloplasmin in urine increased during the luteal phase. Similar to the known lost pregnancies, the temporal pattern of change in urinary ceruloplasmin during the luteal phase deviated from the term pregnancies suggesting that these cycles may have also been lost pregnancies. Among giant pandas in captivity, it has been presumed that there is a high rate of pregnancy loss and our results are the first to provide evidence supporting this notion.

  8. Plasma protein thiols, ceruloplasmin, C-reactive protein and red blood cell acetylcholinesterase in patients undergoing intrauterine insemination

    Directory of Open Access Journals (Sweden)

    Krishnananda Prabhu

    2009-01-01

    Full Text Available Objective: To estimate acetylcholinesterase (AChE, protein thiols (PT, ceruloplasmin (CP and C-reactive proteins (CRPs to assess any change in their levels following intrauterine insemination (IUI. Materials and Methods: Forty-two patients aged 31 ± 4.65 years (mean ± SD with primary infertility selected for IUI. All of them had induced ovulation with clomiphene citrate 50 mg from day 2 to day 6. After taking the consent, 2 ml of blood was withdrawn before and after 24 h of IUI for biochemical estimations. Results: We observed a significant decrease in plasma CP, PT and RBC AChE ( P < 0.001 following IUI compared with the respective pre-procedure levels. Highly sensitive CRP showed a marginal increase after IUI. Conclusion: Fluctuations in levels of the above parameters point to their role in the female reproductive system and in the outcome of the IUI.

  9. Significance of detection of serum ceruloplasmin in diagnosis of MODS patients%MODS患者血清铜蓝蛋白测定的意义

    Institute of Scientific and Technical Information of China (English)

    胡志华; 陈勉; 饶平

    2012-01-01

    Objective To investigate the significance of dynamic changes of serum ceruloplasmin in multiple organ dysfunction syndrome (MODS)for early diagnosis and treatment of the cases. Methods The levels of serum ceruloplasmin (CP),high-sensitivity C-reactive protein (CRCP), α-acid glycoprotein (AAG in 60 MODS patients,MODS group)on the day of admission and 30 cases of non-MODS patients (control group)were determined and analyzed. The APACHE Ⅱ ,MODS score were compared between two groups. Results The APACHE Ⅱ , MODS score, based on the levels of serum ceruloplasmin,high-sensitivity C-reactive protein and α -acid glyeoprotein in MODS group were significantly different compared to the control group (P<0.05). Compared with non-infection group,ceruloplasmin and C-reactive protein were significantly increased,the difference was significant (P<0.05). Conclusion In MODS patients,ceruloplasmin and C-reactive protein test can help early diagnose the syndrome; ceruloplasmin and C-reactive protein monitoring also can help determine the cause of MODS useful for guiding treatment of MODS patients.%目的 探讨血清中铜蓝蛋白动态变化对多器官功能障碍综合症(MODS)患者早期诊断和病因判断的价值.方法 于入院当天分别检测60例MODS患者(MODS组)、30例非MODS患者(对照组)血清铜蓝蛋白(CP)、超敏C反应蛋白(CRCP)、α-酸性糖蛋白(AAG)的含量,并对入选的住院患者进行APACHEⅡ和MODS评分.结果 MODS组与对照组相比其APACHEⅡ、MODS评分、血清铜蓝蛋白(CP)、超敏C反应蛋白(cRCP)、α-酸性糖蛋白(AAG)的比较,差异有统计学意义(P<0.05).非感染组与感染组相比较,铜蓝蛋白与超敏C反应蛋白均明显升高,差异有统计学意义(P<0.05).结论 MODS患者铜蓝蛋白、超敏C反应蛋白、α-酸性糖蛋白的检测有助于MODS早期诊断;铜蓝蛋白及超敏C反应蛋白的监测有助于MODS病因的初步判断.

  10. Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows

    OpenAIRE

    Akalın Pınar Peker; Ataseven Veysel Soydal; Fırat Doğan; Ergün Yaşar; Başpınar Nuri; Özcan Oğuzhan

    2015-01-01

    The aim of this study was to determine the ceruloplasmin (Cp) and vitamin C concentrations, the total antioxidant status (TAS), and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV). Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL), whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST) (P = 0.003) and Cp concentrations (P = 0.03) decreas...

  11. Myeloperoxidase-Related Chlorination Activity Is Positively Associated with Circulating Ceruloplasmin in Chronic Heart Failure Patients: Relationship with Neurohormonal, Inflammatory, and Nutritional Parameters

    Directory of Open Access Journals (Sweden)

    Aderville Cabassi

    2015-01-01

    Full Text Available Rationale. Heart failure (HF is accompanied by the development of an imbalance between oxygen- and nitric oxide-derived free radical production leading to protein nitration. Both chlorinating and peroxidase cycle of Myeloperoxidase (MPO contribute to oxidative and nitrosative stress and are involved in tyrosine nitration of protein. Ceruloplasmin (Cp has antioxidant function through its ferroxidase I (FeOxI activity and has recently been proposed as a physiological defense mechanism against MPO inappropriate actions. Objective. We investigated the relationship between plasma MPO-related chlorinating activity, Cp and FeOxI, and nitrosative stress, inflammatory, neurohormonal, and nutritional biomarkers in HF patients. Methods and Results. In chronic HF patients (n=81, 76 ± 9 years, NYHA Class II (26; Class III (29; Class IV (26 and age-matched controls (n=17, 75 ± 11 years, CTR, plasma MPO chlorinating activity, Cp, FeOxI, nitrated protein, free Malondialdehyde, BNP, norepinephrine, hsCRP, albumin, and prealbumin were measured. Plasma MPO chlorinating activity, Cp, BNP, norepinephrine, and hsCRP were increased in HF versus CTR. FeOxI, albumin, and prealbumin were decreased in HF. MPO-related chlorinating activity was positively related to Cp (r= 0.363, P<0.001, nitrated protein, hsCRP, and BNP and inversely to albumin. Conclusions. Plasma MPO chlorinated activity is increased in elderly chronic HF patients and positively associated with Cp, inflammatory, neurohormonal, and nitrosative parameters suggesting a role in HF progression.

  12. Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows

    Directory of Open Access Journals (Sweden)

    Akalın Pınar Peker

    2015-09-01

    Full Text Available The aim of this study was to determine the ceruloplasmin (Cp and vitamin C concentrations, the total antioxidant status (TAS, and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV. Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL, whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST (P = 0.003 and Cp concentrations (P = 0.03 decreased (65.17 ± 5.03 and 7.70 ± 0.72 respectively in BLV-infected cows, as compared to healthy animals (100.67 ± 11.50 and 10.40 ± 0.70 respectively. A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unknown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.

  13. Functional link between ferroxidase activity of ceruloplasmin and protective effect of apo-lactoferrin: studying rats kept on a silver chloride diet.

    Science.gov (United States)

    Kostevich, Valeria A; Sokolov, Alexey V; Kozlov, Stanislav O; Vlasenko, Anna Yu; Kolmakov, Nikolay N; Zakharova, Elena T; Vasilyev, Vadim B

    2016-08-01

    Strongly pronounced argyrosis caused by adding AgCl to the feed of laboratory rats efficiently mimics the deficiency of ceruloplasmin (CP) ferroxidase activity. Bringing the concentration of AgCl in the feedstuff of lactating rats to 250 mg % and keeping their progeny (Ag-rats) for 3 months on the same silver-containing feed provided the serum iron content 1.4 times lower than that in the control group. Besides, the ferroxidase activity of CP dropped to zero. In CP purified from sera of Ag-rats two copper ions were substituted with two silver ions. Using rat models of both post-hemorrhagic and hemolytic anemia we showed that the deficiency of CP ferroxidase activity in Ag-rats affects the iron content in serum, though does not prevent the recovery of hemoglobin level accompanied by exhaustion of iron caches in liver and spleen. When apo-lactoferrin (apo-LF) was administered to Ag-rats suffering from either post-hemorrhagic or hemolytic anemia, both hemoglobin and serum iron were restored more rapidly than in the control animals. In independent experiments Ag-rats were compared with those fed on regular diet and the former displayed a prolonged 3-day stabilization of hypoxia-inducible factors 1 and 2 alpha (HIF-1a and HIF-2a) along with an increased serum concentration of erythropoietin. Introduction to Ag-rats of active CP separately or together with apo-LF reduced that effect to 1 day only. It is concluded that saturation of apo-LF with iron, provided by active CP, can strongly affect its protective capacity. PMID:27377930

  14. Delayed translational silencing of ceruloplasmin transcript in gamma interferon-activated U937 monocytic cells: role of the 3' untranslated region

    Science.gov (United States)

    Mazumder, B.; Fox, P. L.

    1999-01-01

    Ceruloplasmin (Cp) is an acute-phase protein with ferroxidase, amine oxidase, and pro- and antioxidant activities. The primary site of Cp synthesis in human adults is the liver, but it is also synthesized by cells of monocytic origin. We have shown that gamma interferon (IFN-gamma) induces the synthesis of Cp mRNA and protein in monocytic cells. We now report that the induced synthesis of Cp is terminated by a mechanism involving transcript-specific translational repression. Cp protein synthesis in U937 cells ceased after 16 h even in the presence of abundant Cp mRNA. RNA isolated from cells treated with IFN-gamma for 24 h exhibited a high in vitro translation rate, suggesting that the transcript was not defective. Ribosomal association of Cp mRNA was examined by sucrose centrifugation. When Cp synthesis was high, i.e., after 8 h of IFN-gamma treatment, Cp mRNA was primarily associated with polyribosomes. However, after 24 h, when Cp synthesis was low, Cp mRNA was primarily in the nonpolyribosomal fraction. Cytosolic extracts from cells treated with IFN-gamma for 24 h, but not for 8 h, contained a factor which blocked in vitro Cp translation. Inhibitor expression was cell type specific and present in extracts of human cells of myeloid origin, but not in several nonmyeloid cells. The inhibitory factor bound to the 3' untranslated region (3'-UTR) of Cp mRNA, as shown by restoration of in vitro translation by synthetic 3'-UTR added as a "decoy" and detection of a binding complex by RNA gel shift analysis. Deletion mapping of the Cp 3'-UTR indicated an internal 100-nucleotide region of the Cp 3'-UTR that was required for complex formation as well as for silencing of translation. Although transcript-specific translational control is common during development and differentiation and global translational control occurs during responses to cytokines and stress, to our knowledge, this is the first report of translational silencing of a specific transcript following cytokine

  15. Avaliação dos níveis de hiper-homocisteinemia, vitamina E, selênio, cobre, ceruloplasmina e ferritina em pacientes com diagnóstico de acidente vascular cerebral isquêmico Assesment of homocysteine, vitamin E, selenium, copper, ceruloplasmin and ferritin levels in patients with ischemic stroke diagnosis

    Directory of Open Access Journals (Sweden)

    Lillian Harboe-Gonçalves

    2007-02-01

    Full Text Available OBJETIVO: Avaliar níveis sangüíneos de hiper-homocisteinemia, vitamina E, selênio, cobre, ceruloplasmina e ferritina em pacientes com diagnóstico de acidente vascular cerebral isquêmico (AVCI. CASUÍSTICA, MATERIAIS E MÉTODOS: Estudo transversal analítico de 104 pacientes diagnosticados com AVCI e 98 controles. Dosagem de homocisteína e vitamina E feita por cromatografia líquida de alta performance. O selênio foi dosado por tecnologia de espectrometria de massa por plasma indutivamente acoplado; o cobre o foi por colorimetria; a ceruloplasmina, por nefelometria; e a ferritina, por imunoensaio enzimático conjugado à fluorescência (Elfa. Análise estatística feita com testes t de Student e análise de variância. RESULTADOS: Encontrada hiper-homocisteinemia em 43% dos pacientes e 13% dos controles. Os valores obtidos nas dosagens de vitamina E, cobre, ceruloplasmina e ferritina foram significativamente maiores no grupo paciente do que no grupo controle. Quanto ao selênio, a diferença entre os dois grupos não foi significativa. DISCUSSÃO: Níveis de hiper-homocisteinemia foram maiores que os encontrados na literatura, podendo ser conseqüência da genética ou dos hábitos alimentares da população. Os resultados encontrados para vitamina E, selênio, cobre, ceruloplasmina e ferritina coincidem com os de alguns trabalhos publicados, mas o modelo deste estudo não avalia se os níveis de vitamina E e selênio foram alterados pelo AVCI ou por mudanças nos hábitos dos pacientes. A ferritina e a ceruloplasmina podem ser marcadores de AVCI, e não fator causal da sua patogênese. CONCLUSÃO: A hiper-homocisteinemia é mais prevalente nos pacientes diagnosticados com AVCI. Os níveis de vitamina E, cobre, ceruloplasmina e ferritina mais elevados encontrados nos pacientes não podem ser atribuídos à ocorrência de AVCI.OBJECTIVE: To assess blood levels of hyperhomocysteinaemia, vitamin E, selenium, copper, ceruloplasmin and

  16. Interaction of [Rh2(O2CCH3)4(H2O)2] and [Rh2(O2CCH(OH)Ph)2(phen)2(H2O)2](O2C-CH(OH)Ph)2 With Sulfhydryl Compounds and Ceruloplasmin

    OpenAIRE

    Jakimowicz, Piotr; Ostropolska, Lucja; Pruchnik, Florian P.

    2000-01-01

    The interaction of binuclear rhodium(II) complexes [Rh2(OOCCH3)4(H2O)2], [Rh2{OOCCH(OH)Ph}2(phen)2(H2O)2] {OOCCH(OH)Ph}2, [Rh2(OOCCH3)2(bpy)2(H2O)2](OOCCH3)2 and [Rh2Cl2(OOCMe)2(bpy)2](3H2O) with ceruloplasmin, cysteine, glutathione and coenzyme A have been investigated using. UV-Vis and CD spectroscopies. The complexes containing phen or bpy at pH = 7.4 and 4.0 are readily reduced with sulfhydryl compounds, while rhodium(II) acetate is relatively stable in these conditions. Complex [Rh2{OOCC...

  17. Ceruloplasmina y su importancia clínica como factor indicador del riesgo cardiovascular en una población de escolares de Granada Ceruloplasmin and its clinical relevance as an indactor of cardiovascular risk factor in a school population of Granada

    Directory of Open Access Journals (Sweden)

    Mª J. Aguilar Cordero

    2011-06-01

    Full Text Available La ceruloplasmina también conocida como ferroxidasa, pertenece a la familia de las proteínas sensibles a la inflamación, siendo su función principal la de transportar el cobre en la sangre. Si bien, además de esta función transportadora, en la actualidad, son numerosos los estudios que han intentado hacer uso de la determinación de sus concentraciones séricas, como un indicador predictivo del riesgo de padecer trastornos cardiovasculares en pacientes que presentan sobrepeso u obesidad. Los resultados obtenidos en este estudio confirman la existencia de una correlación significativa entre los niveles séricos de ceruloplasmina y el estado nutricional de los sujetos, lo que significa que para la población de escolares valorada, las concentraciones séricas de esta proteína suponen un importante factor para predecir el riesgo de padecer trastornos cardiovasculares.Also known as ferroxidase ceruloplasmin, belongs to the family of inflammation-sensitive proteins, and its main function to transport copper in the blood. Although, in addition to this transport function, at present, there are numerous studies that have attempted to use the determination of serum concentrations as a predictive indicator of cardiovascular risk in patients who are overweight or obese. The results of this study confirm the existence of a significant correlation between serum ceruloplasmin and nutritional status of the subjects, which means that for the population of students assessed, serum levels of this protein are an important predictor the risk of cardiovascular disease.

  18. Determinação sérica de haptoglobina, ceruloplasmina e alfa-glicoproteína ácida em cães com gastrenterite hemorrágica Determination of serum haptoglobin, ceruloplasmin and acid alpha-glycoprotein in dogs with haemorrhagic gastroenteritis

    Directory of Open Access Journals (Sweden)

    Márcia Mery Kogika

    2003-06-01

    confidence for the ceruloplasmin (p <0.0478 when compared to the control group. In conclusion, dogs with haemorrhagic gastroenteritis during the leukopenic phase showed high serum levels of haptoglobin, ceruloplasmin and alpha-acid-glycoprotein.

  19. Estudio de los niveles séricos de leptina, ceruloplasmina y lipoproteína (a como indicadores del riesgo cardiovascular en una población de adolescentes de Granada (España Study of the serum levels of leptin, ceruloplasmin and lipoprotein (a as indicators of cardiovascular risk in a population of adolescents in Granada (Spain

    Directory of Open Access Journals (Sweden)

    Mª J. Aguilar Cordero

    2011-10-01

    Full Text Available Han sido numerosos los estudios orientados a establecer una relación entre los valores séricos de biomoléculas como la leptina, ceruloplasmina, lipoproteína (a y el estado nutricional y niveles de presión arterial sistólica y diastólica en sujetos con problemas de sobrepeso u obesidad, no siendo concluyentes en muchos casos los datos alcanzados. Los resultados obtenidos en este estudio confirman la existencia de una asociación estadísticamente significativa entre los niveles séricos de dichas biomoléculas el estado nutricional de los sujetos y los niveles de presión arterial sistólica y diastólica. Resultado de ello y para la población de adolescentes con sobrepeso y obesidad estudiada, la valoración de las concentraciones séricas de estas biomoléculas resultó ser un importante instrumento para identificar aquellos sujetos con un riesgo elevado de padecer trastornos cardiovasculares, fundamentalmente derivados de un estatus hipertensivo.Numerous studies have focused on establishing a relation between the serum values of biomolecules such as leptin, ceruloplasmin, and lipoprotein (a, and the nutritional state and levels of diastolic and systolic blood pressure in subjects with problems of overweight or obesity. However, in many cases, the results obtained have not been conclusive. The results of our study confirm the existence of a statistically significant association between the serum levels of these biomolecules, the nutritional state of the subjects, and levels of diastolic and systolic blood pressure. For the population of overweight and obese adolescents studied, the evaluation of the serum concentrations of these biomolecules was found to be an important instrument that could be used to identify those subjects with an elevated risk of suffering cardiovascular disorders basically derived from a hypertensive status.

  20. Determinação sérica de haptoglobina, ceruloplasmina, α1-glicoproteína ácida, transferrina e α1-antitripsina, em equinos com cólica Determination of serum haptoglobin, ceruloplasmin, α1-acid glycoprotein, transferrin and α1-antitrypsin in colic horses

    Directory of Open Access Journals (Sweden)

    Paula Alessandra Di Filippo

    2011-12-01

    Full Text Available Foram examinados 46 equinos adultos, 6 hígidos (G1 e 40 com cólica, submetidos à laparotomia. Vinte apresentavam lesões no intestino grosso (G2 e 20 no intestino delgado (G3. Avaliaram-se os teores séricos das proteínas de fase aguda: haptoglobina, ceruloplasmina, antitripsina, transferrina e glicoproteína ácida, antes e até sete dias após a laparotomia. Após centrifugação e fracionamento das amostras, as proteínas de fase aguda foram separadas por eletroforese em gel de poliacrilamida contendo SDS-PAGE, e suas concentrações determinadas por densitometria computadorizada. Constatou-se elevação dos valores das proteínas de fase aguda nos animais com cólica, antes e após laparotomia, porém com valores mais elevados, precoces e persistentes nos animais do G3. Os resultados deveram-se ao processo inflamatório intestinal, desencadeado pela lesão entérica e indicam que o proteinograma sérico pode auxiliar na identificação do segmento intestinal obstruído e, consequentemente, na elaboração do prognóstico de equinos com cólica.Forty six equines were examined, 6 were healthy (G1 and 40 with colic, submitted to laparotomy. Twenty were showing lesions on the large intestine (G2 and 20 lesions on the small intestine (G3. The serum concentrations of acute phase proteins: haptoglobin, ceruloplasmin, antitrypsin, transferrin, and - acid glycoprotein before and until 7 days after laparotomy. After centrifugation and fractioning of the samples, the acute phase proteins were individualized by electrophoresis on polyacrylamide gel containing SDS-PAGE, and the concentrations were determined by computerized densitometry. The increase was verified on the levels of acute phase proteins from animals with colic, before and after laparotomy, however with higher levels, premature and persistent on animals from G3. The results were due to the process of intestinal inflammation, caused by enteric injury and indicate that the serum protein

  1. CA-125 and Ceruloplasmin Levels in Ovarian Cancer Patients

    OpenAIRE

    Mangala Hegde; Yousef Rezaei Chianeh; Jeevan Shetty; Donald J. Fernandes; Pragna Rao

    2015-01-01

    Purpose: The initial stage of proliferation of epithelial ovarian carcinoma (EOCa) is usually asymptomatic. Due to the lack of sensitive and reliable markers in majority of patients the disease is widespread at the time of diagnosis. The reliable serum biomarkers currently accepted is CA125 but there is limitation in case of sensitivity of CA125 as it is detectable only in 50% of patients in stage I and 80% of patients with advanced stage. We have investigated a correlation between serum CA12...

  2. Transfer of copper from a chelated 67Cu-antibody conjugate to ceruloplasmin in lymphoma patients

    International Nuclear Information System (INIS)

    The Lym-1 monoclonal antibody was conjugated with the bifunctional chelating agent 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N',N'',N' -tetraacetic acid (BAT), using 2IT as a linker, and radiolabeled with 67Cu to make the radiopharmaceutical, 67Cu-2IT-BAT-Lym-1. Ten patients received a total of 18 doses of 67Cu-2IT-BAT-Lym-1 as targeted, systemic radiotherapy. The beta phase of blood clearance, when corrected for 67Cu decay, was positive or flat, a phenomenon not observed in similar patients treated with 131I-Lym-1. The flat beta phase of blood clearance suggested recycling of 67Cu from 67Cu-2IT-BAT-Lym-1 to another plasma protein. Therefore, the amount of 67Cu transferred from the radiopharmaceutical to CP, Alb, and TF was measured using affinity-purified polyclonal antibodies. The fraction of plasma 67Cu precipitated by anti-human CP increased daily; most blood radioactivity was 67Cu-CP after a median of 4 days (range 2-7 days). The transfer of 67Cu to CP was observed in all patients and was consistent from dose to dose within the same patient. An average of 2.8±1.5% (range 0.8-7.8%) of the 67Cu dose (%ID) was transferred to CP. The release rate of 67Cu-CP from the liver into the blood was 0.9±0.4 %ID/day for the first 3 days. The 67Cu-CP effective clearance half-life was 3.7 ± 0.7 days. Subtraction of the 67Cu-CP activity from the total blood radioactivity yielded a biphasic blood clearance similar to that obtained for patients given 131I-Lym-1. Cu-67-CP increased the AUC for whole blood by 24 ± 10%. The %ID of 67Cu recycled correlated with GGT, ALT, and alkaline phosphatase levels; r=0.958 (p67Cu-2IT-BAT-Lym-1 and recycles a small fraction of the 67Cu, transferring it to CP

  3. Serum Levels of Copper, Ceruloplasmin and Angiotensin Converting Enzyme among Silicotic and Non-Silicotic Workers

    Directory of Open Access Journals (Sweden)

    Safia Beshir

    2015-06-01

    CONCLUSION: Since respirable dust exposure-linked lung fibrosis disease is non-curable, the biochemical parameters (Cu, ACE and Cp can be used as exposure biomarkers to silica dust, providing a better way for early diagnosis of this deadly disease. Down regulating the inflammatory responses could potentially reduce the adverse clinical pulmonary effects of air pollution.

  4. The Effects of Plasma Vitamin C and Ceruloplasmin Levels and Exogen Vitamin C Supplementation on Reproduction in Sheep

    OpenAIRE

    HALİLOĞLU, Seyfullah; SERPEK, Behiç

    2000-01-01

    The effects of vitamin C on progesterone and oestradiol 17b synthesis, and fertility rate were studied in Merino sheep. A total of 60 sheep, aged between 2 and 7 years, of which 30 had low, and the other 30 had high plasma vitamin C levels were used. Each low and high vitamin C group was divided into 2 subgroups. Each subgroup had equal numbers of sheep. Subgroups 2 and 4 served as controls in the low and high vitamin C groups, respectively. 500 mg of vitamin C was injected intramuscularly...

  5. Effect of uv laser radiation on copper-proteins

    Science.gov (United States)

    Bacci, M.; Fabeni, P.; Linari, R.; Pazzi, G. P.

    The results of irradiation studies of human ceruloplasmin, mushroom tyrosinase and Limuluspolyphemus hemocyanin with a nitrogen laser (Λ = 337.1 nm) are presented. In all the proteins we have considered the absorption band at 330 nm, characteristic of dimer Type 3 copper centres, decreases, while an increase of the absorbance at 280 nm is observed. Moreover a new band at about 400 nm appears in irradiated ceruloplasmin. Finally sharp oscillations of the absorbance at 610 and 794 nm are recorded in ceruloplasmin, when the irradiation is switched off.

  6. Estudio de los niveles séricos de leptina, ceruloplasmina y lipoproteína (a) como indicadores del riesgo cardiovascular en una población de adolescentes de Granada (España) Study of the serum levels of leptin, ceruloplasmin and lipoprotein (a) as indicators of cardiovascular risk in a population of adolescents in Granada (Spain)

    OpenAIRE

    Mª. J. Aguilar Cordero; E. González Jiménez; J. Álvarez Ferré; C.A. Padilla López; F. Rivas García; Perona, J. S.; R. García Aguilar

    2011-01-01

    Han sido numerosos los estudios orientados a establecer una relación entre los valores séricos de biomoléculas como la leptina, ceruloplasmina, lipoproteína (a) y el estado nutricional y niveles de presión arterial sistólica y diastólica en sujetos con problemas de sobrepeso u obesidad, no siendo concluyentes en muchos casos los datos alcanzados. Los resultados obtenidos en este estudio confirman la existencia de una asociación estadísticamente significativa entre los niveles séricos de dicha...

  7. Copper Test

    Science.gov (United States)

    ... mean? Copper test results must be evaluated in context and are usually compared to ceruloplasmin levels . Abnormal ... Health Professionals ©2001 - by American Association for Clinical Chemistry • Contact Us | Terms of Use | Privacy We comply ...

  8. Hepatic but not brain iron is rapidly chelated by deferasirox in aceruloplasminemia due to a novel gene mutation

    OpenAIRE

    Finkenstedt, Armin; Wolf, Elisabeth; Höfner, Elmar; Gasser, Bethina Isasi; Bösch, Sylvia; Bakry, Rania; Creus, Marc; Kremser, Christian; Schocke, Michael; Theurl, Milan; Moser, Patrizia; Schranz, Melanie; Bonn, Guenther; Poewe, Werner; Vogel, Wolfgang

    2010-01-01

    Background & Aims Aceruloplasminemia is a rare autosomal recessive neurodegenerative disease associated with brain and liver iron accumulation which typically presents with movement disorders, retinal degeneration, and diabetes mellitus. Ceruloplasmin is a multi-copper ferroxidase that is secreted into plasma and facilitates cellular iron export and iron binding to transferrin. Results A novel homozygous ceruloplasmin gene mutation, c.2554+1G>T, was identified as the cause of aceruloplasminem...

  9. Copper transport.

    Science.gov (United States)

    Linder, M C; Wooten, L; Cerveza, P; Cotton, S; Shulze, R; Lomeli, N

    1998-05-01

    In adult humans, the net absorption of dietary copper is approximately 1 mg/d. Dietary copper joins some 4-5 mg of endogenous copper flowing into the gastrointestinal tract through various digestive juices. Most of this copper returns to the circulation and to the tissues (including liver) that formed them. Much lower amounts of copper flow into and out of other major parts of the body (including heart, skeletal muscle, and brain). Newly absorbed copper is transported to body tissues in two phases, borne primarily by plasma protein carriers (albumin, transcuprein, and ceruloplasmin). In the first phase, copper goes from the intestine to the liver and kidney; in the second phase, copper usually goes from the liver (and perhaps also the kidney) to other organs. Ceruloplasmin plays a role in this second phase. Alternatively, liver copper can also exit via the bile, and in a form that is less easily reabsorbed. Copper is also present in and transported by other body fluids, including those bathing the brain and central nervous system and surrounding the fetus in the amniotic sac. Ceruloplasmin is present in these fluids and may also be involved in copper transport there. The concentrations of copper and ceruloplasmin in milk vary with lactational stage. Parallel changes occur in ceruloplasmin messenger RNA expression in the mammary gland (as determined in pigs). Copper in milk ceruloplasmin appears to be particularly available for absorption, at least in rats. PMID:9587137

  10. 营养补充对运动性低血色素大鼠血清、组织铜蓝蛋白及血清铁等铁代谢指标变化规律的研究%Effect of nutrition supplement on serum and tissue ceruloplasmin, serum iron and other index related to iron metabolism when sports hypochrosis happens in rats

    Institute of Scientific and Technical Information of China (English)

    曹建民; 田野; 赵杰修; 金丽; 郑弘溶; 李进挺; 赵宁宁

    2005-01-01

    为了探寻营养补充对运动性低血色素大鼠铜蓝蛋白与铁代谢指标变化的规律,以大鼠为实验对象,采用跑台作为运动方式建立运动性低血色素大鼠模型,并对大鼠进行营养干预,对其血清、组织中的铜蓝蛋白以及铁代谢相关指标进行测定.实验结果表明:长时间的运动训练导致运动性低血色素大鼠血清铜蓝蛋白、转铁蛋白明显上升,铁蛋白明显下降,而且与血清铁浓度具有较高的相关性,说明血清铜蓝蛋白、铁蛋白、转铁蛋白可以反映机体铁代谢情况;运动训练导致运动性低血色素大鼠组织铜蓝蛋白含量与血清铜蓝蛋白变化不一致;营养补剂的干预显著降低了运动性低血色素大鼠血清铜蓝蛋白、转铁蛋白含量,提高了血清铁及铁蛋白含量,表明抗贫血铁制剂复合营养补剂对提高大鼠铁贮备和维持大鼠铁代谢稳定具有积极作用.

  11. On the transfer of serum proteins to the rat intestinal juice

    DEFF Research Database (Denmark)

    Andersen, Vibeke; Hansen, G H; Olsen, J;

    1994-01-01

    The in vivo pattern of serum proteins in the rat small-intestinal juice was characterized by crossed immunoelectrophoresis. Immunoglobulins and albumin, alpha-1-antitrypsin, transferrin, and orosomucoid were present. Larger serum proteins were absent (ceruloplasmin, haptoglobin, alpha-1-macroglob......The in vivo pattern of serum proteins in the rat small-intestinal juice was characterized by crossed immunoelectrophoresis. Immunoglobulins and albumin, alpha-1-antitrypsin, transferrin, and orosomucoid were present. Larger serum proteins were absent (ceruloplasmin, haptoglobin, alpha-1...... proteins in the intestinal juice is a selective passage through the capillary wall followed by passive intercellular transport via delivery of the serum in the interstitial space during disintegration of the enterocytes....

  12. Chemical and enzymatic oxidative coupling of 5-hydroxy-N,N-dimethyltryptamine with amines.

    Science.gov (United States)

    Babin, F; Huynh-Dinh, T

    1987-07-01

    As part of a program aiming to obtain a covalent labeling of serotoninergic receptors we have studied the oxidative coupling of serotonin derivatives with amino compounds. The oxidation of bufotenine (2) by MnO2 and human ceruloplasmin followed by the Michael type addition with dansylcadaverine and dansyllysine gave a fluorescent adduct identified as fused oxazole structure 4. PMID:3599029

  13. Copper transport in rats involving a new plasma protein

    International Nuclear Information System (INIS)

    The time course of distribution of high-specific activity 67CuCl2 to tissues and plasma components was followed in adult, female rats. Immediately after intubation or injection, tracer 67Cu associated with two components of the blood plasma separable on columns of Sephadex G-150: albumin and another (larger) component, which was not ceruloplasmin. The latter, tentatively named transcuprein, had an apparent molecular weight of 270,000 and a high affinity for Cu2+, as judged by processing through Chelex-100, dilution, and exchange with albumin copper, in vitro and in vivo. It was capable of donating copper to tumor cells in serum-free medium. Analysis of ''cold'' plasma by furnace atomic absorption confirmed the presence of 10-15% of plasma copper in this peak. Plots of percent dose and 67Cu specific activity against time showed that copper followed a very specific pathway after binding to albumin and transcuprein, entering mainly the liver, then reappearing in the plasma on ceruloplasmin, and then achieving peak distribution in peripheral tissues (muscles, brain, etc.). 67Cu disappeared from liver and kidney with an apparent half-life of 4.5 days, the same exponential rate found for whole body turnover. Apparent turnover of ceruloplasmin copper was more rapid. Even after 7-12 days, tracer copper in plasma was still found exclusively with ceruloplasmin. The results indicate that copper follows a carefully prescribed path, on entering the blood and binding to a new transport protein

  14. Neonatal erythroderma as a first manifestation of Menkes disease

    DEFF Research Database (Denmark)

    Galve, Javier; Vicente, Asunción; González-Enseñat, María Antonia;

    2012-01-01

    Menkes disease is an X-linked recessive lethal multisystemic disorder of copper metabolism. Progressive neurodegeneration, connective tissue disturbances, and peculiar kinky hair are the main manifestations. The low serum copper and ceruloplasmin suggests the diagnosis, which is confirmed by muta...... possible to establish prompt treatment with parenteral copper-histidine supplementation to improve prognosis....

  15. Rapid and Sensitive Detection of Protein Biomarker Using a Portable Fluorescence Biosensor based on Quantum Dots and a Lateral Flow Test Strip

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhaohui; Wang, Ying; Wang, Jun; Tang, Zhiwen; Pounds, Joel G.; Lin, Yuehe

    2010-08-15

    A portable fluorescence biosensor with rapid and ultrasensitive response for trace protein has been built up with quantum dots and lateral flow test strip. The superior signal brightness and high photostability of quantum dots are combined with the promising advantages of lateral flow test strip and resulted in high sensitivity, selectivity and speedy for protein detection. Nitrated ceruloplasmin, a significant biomarker for cardiovascular disease, lung cancer and stress response to smoking, was used as model protein to demonstrate the good performances of this proposed Qdot-based lateral flow test strip. Quantitative detection of nitrated ceruloplasmin was realized by recording the fluorescence intensity of quantum dots captured on the test line. Under optimal conditions, this portable fluorescence biosensor displays rapid responses for nitrated ceruloplasmin in wide dynamic range with a detection limit of 0.1ng/mL (S/N=3). Furthermore, the biosensor was successfully utilized for spiked human plasma sample detection with the concentration as low as 1ng/mL. The results demonstrate that the quantum dot-based lateral flow test strip is capable for rapid, sensitive, and quantitative detection of nitrated ceruloplasmin and hold a great promise for point-of-care and in field analysis of other protein biomarkers.

  16. Copper transport from mother to fetus, at the end of gestation, in rats

    International Nuclear Information System (INIS)

    The authors have followed the fate of 67Cu in pregnant rats, from 1-4 days before the end of gestation, after its intravenous infusion in the form of in vivo labeled ceruloplasmin (Cp) or 67Cu(II)-treated rat serum. One and 4 hours after administration, radioactivity administered as Cp was still with Cp in the maternal circulation and that of ionic copper was still associated with transcuprein and albumin (Tc and Alb). On the basis of % dose, Cp appeared to be more effective than Tc + Alb as a source of copper for the placenta and fetus. In contrast, maternal liver took up less 67Cu from Cp than from Tc + Alb. Four hours after administration, about 8% of the 67Cu administered as Cp had been taken up by the placenta and fetuses compared with about 2% in the case of 67Cu from Tc + Alb. The amounts of 67Cu in fetuses and fetal liver increased with time. Initial trials indicated that Cu administered as Cp became attached mainly to Tc in the fetal circulation, but was also associated with Alb and with Cp, at least by 4 hours. mRNA for ceruloplasmin was detected in maternal liver, placenta, and fetal liver, where it was present primarily on ER-bound (rather than free) polyribosomes. They conclude that normally ceruloplasmin is probably the main source of copper for the fetus, at least in the rat, although transcuprein and albumin can substitute as sources; and after entering the placenta, copper from ceruloplasmin may be transferred to transcuprein and albumin in the fetal circulation, from whence it enters the liver for storage and incorporation into fetal ceruloplasmin

  17. 一例Wilson病的影像学表现%Imaging findings of liver in the Wilson's disease:a case report

    Institute of Scientific and Technical Information of China (English)

    孔文韬; 张炜炜; 桑剑锋; 陈骏

    2011-01-01

    @@ Introduction Wilson's disease is a rare inherited disorder of copper metabolism which is characterized by the inability to secrete the copper-ceruloplasmin complex into plasma and free copper into the bile.In the liver,the copper deposits in the periportal regions and incites an inflammatory reaction, resulting in chronic liver disease[1].%IntroductionWilson 's disease is a rare inherited disorder of copper metabolism which is characterized by the inability to secrete the copper ceruloplasmin complex into plasma and free copper into the bile.In the liver,the copper deposits in the peliportal regiorks and incites an inflammatory reaction,resulting in chronic liver disease[ 1].Untreated Wilson 's disease is progressive and fatal,so the early diagnosis is important for working out treatment strategy [2].

  18. R778L, H1069Q, and I1102T mutation study in neurologic Wilson disease

    OpenAIRE

    Kalita Jayantee; Somarajan Bindu; Misra Usha; Mittal Balraj

    2010-01-01

    There is paucity of the studies on mutations in neurologic Wilson disease (WD) in India. We studied H1069Q, R778L, I1102T mutations in 26 patients with neurologic WD from 25 families in north India. The basis of diagnosis of neurologic WD was clinical, Kayser-Fleischer (KF) ring, and ceruloplasmin. Data collected included: family history, clinical characteristics, laboratory data, ultrasound findings, magnetic resonance imaging (MRI) findings, and severity of the disease. DNA was isolated fro...

  19. Severe neuropsychiatric presentation of Wilson's disease

    OpenAIRE

    Chakor, Rahul T.; Santhosh, N. S.

    2011-01-01

    Wilson's disease (WD) is a relatively rare disease of copper metabolism. The diagnosis is often missed initially. The presentation is usually neurologic or hepatic, seen in 40% of patients. Psychiatric presentation of WD is reported in only 15% of patients. We present a 32-year-old patient with severe psychiatric manifestations. On examination, he had mild rest and postural tremors and a KF ring was seen. Serum ceruloplasmin was low and 24-hour urinary copper was elevated. The patient respond...

  20. Efficacy of topical honey therapy against silver sulphadiazine treatment in burns: A biochemical study

    OpenAIRE

    Nagane, N. S.; Ganu, J. V.; Bhagwat, V. R.; Subramanium, M.

    2004-01-01

    Thermal injury is associated with biochemical changes. The present study was undertaken to investigate relation of oxidative free radical generation and related biochemical parameters in burn trauma. The specific aim was to compare the levels of serum lipid peroxide, Ceruloplasmin and Uric Acid in burn patients during treatment with Silver Sulfadiazine Cream and honey therapy. It is a single blind prospective controlled study involving comparison of biochemical changes after treatment with si...

  1. Effect of dietary restriction on sperm characteristic and oxidative status on testicular tissue in young rats exposed to long-term heat stress.

    Science.gov (United States)

    Aydilek, N; Varisli, O; Kocyigit, A; Taskin, A; Kaya, M S

    2015-11-01

    This study was conducted to evaluate the effects of dietary restriction on oxidative status and sperm parameters in rats exposed to long-term heat stress. Forty healthy Sprague-Dawley rats, aged 2.5 month, were divided into four groups of 10 with respect to feeding and temperature regimen (room temperature (22 °C)-ad libitum, room temperature-dietary restriction (40%), high temperature (38 °C)-ad libitum, high temperature-dietary restriction). At the end of the 9th week, some oxidants (lipid hydroperoxide, total oxidant status, oxidative stress index) and antioxidants (total antioxidant status, sulfhydryl groups, ceruloplasmin, paraoxonase and arylesterase activities) were measured in the testis tissue. The concentration, motility, volume, abnormal sperm count, acrosome and membrane integrity of epididymal spermatozoon and intratesticular testosterone levels were evaluated. High temperature did not change oxidative and antioxidative parameters except for sulfhydryl groups and ceruloplasmin, yet it impaired all sperm values. Neither sperm values nor oxidative status apart from sulfhydryl groups, ceruloplasmin and arylesterase was affected by dietary restriction in the testis tissue. These results suggest that long-term heat stress does not have a significant effect on testicular oxidative status, while the spermatozoa are sensitive to heat stress in young rats. Dietary restriction failed to improve the sperm quality and oxidative status except some individual antioxidant parameters; conversely, it decreased intratesticular testosterone level in the young rats exposed to long-term heat stress. PMID:25418546

  2. Assesment of mineral metabolism in patients with HIV-infection, chronic hepatitis с and co-infection HIV/ chronic hepatitis С

    Directory of Open Access Journals (Sweden)

    Yurko Е.М.

    2014-12-01

    Full Text Available Research Objective: Evaluate the indicators of mineral metabolism in HIV-infected patients, patients with chronic hepatitis С and co-infection HIV/HCV. Material and Methods: The content of microelements (zinc (Zn, copper (Cu, iron (Fe in serum was determined by atomic absorption spectrophotometry. The study involved 99 patients: with chronic hepatitis С — 32 patients, with HIV infection — 34 and co-infection of HIV/HCV — 33 patients. Results: Microelements and metal-dependent proteins metabolic disorders as a reduction of the zinc, haptoglobin contents, and an increase of copper, iron, and ceruloplasmin contents were identified in patients with chronic hepatitis С In HIV-infected patients and patients with co-infection HIV/HCV were identified reducing zinc, copper, iron, haptoglobin, ceruloplasmin. in patients with co-infection HIV/HCV compared HIV-infected patients lower zinc, haptoglobin, ceruloplasmin contents (p<0,001 were detected. In patients with co-infected HIV/HCV, as compared with a group of chronic hepatitis С were set lower values of all parameters (p<0,001. Conclusion: Integrated assessment of the degree of deviation from the control of the trace element content and activity metal-dependent enzymes showed that its highest significance was typical for patients with co-infected HIV/HCV, which is higher than that of HIV-infected patients in the 1,2-fold and 2,2-fold in patients with chronic hepatitis С

  3. Neocortex and allocortex respond differentially to cellular stress in vitro and aging in vivo.

    Directory of Open Access Journals (Sweden)

    Jessica M Posimo

    Full Text Available In Parkinson's and Alzheimer's diseases, the allocortex accumulates aggregated proteins such as synuclein and tau well before neocortex. We present a new high-throughput model of this topographic difference by microdissecting neocortex and allocortex from the postnatal rat and treating them in parallel fashion with toxins. Allocortical cultures were more vulnerable to low concentrations of the proteasome inhibitors MG132 and PSI but not the oxidative poison H2O2. The proteasome appeared to be more impaired in allocortex because MG132 raised ubiquitin-conjugated proteins and lowered proteasome activity in allocortex more than neocortex. Allocortex cultures were more vulnerable to MG132 despite greater MG132-induced rises in heat shock protein 70, heme oxygenase 1, and catalase. Proteasome subunits PA700 and PA28 were also higher in allocortex cultures, suggesting compensatory adaptations to greater proteasome impairment. Glutathione and ceruloplasmin were not robustly MG132-responsive and were basally higher in neocortical cultures. Notably, neocortex cultures became as vulnerable to MG132 as allocortex when glutathione synthesis or autophagic defenses were inhibited. Conversely, the glutathione precursor N-acetyl cysteine rendered allocortex resilient to MG132. Glutathione and ceruloplasmin levels were then examined in vivo as a function of age because aging is a natural model of proteasome inhibition and oxidative stress. Allocortical glutathione levels rose linearly with age but were similar to neocortex in whole tissue lysates. In contrast, ceruloplasmin levels were strikingly higher in neocortex at all ages and rose linearly until middle age. PA28 levels rose with age and were higher in allocortex in vivo, also paralleling in vitro data. These neo- and allocortical differences have implications for the many studies that treat the telencephalic mantle as a single unit. Our observations suggest that the topographic progression of protein

  4. Blood plasma proteins and protein fractions in roe deer Capreolus capreolus L.

    Directory of Open Access Journals (Sweden)

    Dorota CYGAN-SZCZEGIELNIAK

    2015-09-01

    Full Text Available The aim of the research was to investigate some selected biochemical blood parameters in roe deer (Capreolus capreolus L.. The experiment covered 15 from 2 to 3-year-old bucks from Kuyavian-Pomeranian Voivodeship. The animals were shot by individual hunters on the shooting grounds during the hunting season of 2008/2009 (in the accordance with the Journal of Laws No 48. The material for the research was blood plasma obtained after centrifuging full, nonhemolyzed blood. The blood was collected from the zygomatic vein directly to the test tubes with EDTA and transported in cooling conditions to the laboratory. After transporting the samples of blood to a certified analytical laboratory, the following elements of the obtained blood plasma were examined: ceruloplasmin . using turbidimetric method; transferrin . using immunoturbimetric method; troponin- using a third generation assay on an Elecsys; total protein, albumin, globulin . using spectrophotometric method and total iron . using colorimetric method. The results were statistically analyzed, i.e. the correlation between the parameters was measured by means of Pearsonfs correlation coefficient. The analysis of the results revealed a number of statistically significant relations between the parameters under the investigation, especially among the compounds directly responsible for metabolism of iron and copper. A statistically important positive correlation was observed between ceruloplasmin and ferritin (r = 0.563; P.0.05 and a negative one between transferrin and troponin (r = -0.609; P.0.05. Moreover, the content of transferrin . an iron-binding protein . was 0.17 g/l, while the concentration of iron was 58 ƒĘmol/l. The content of ceruloplasmin . a protein responsible for metabolism of copper . was very low (0.036 g/l. The level of proteins in the blood plasma of the animals under the research was approximately 72 g/l, with the share of albumins about 46%. The albumin-globulin ratio was 0.86.

  5. Studies with copper-67 and copper-64 in conditioned copper-deficient ruminants: Preliminary results

    International Nuclear Information System (INIS)

    Conditioned copper deficiency was induced in ruminants by feeding a diet of ground alfalfa hay containing, on a dried matter basis, 50 ppm of molybdenum and 0.4% of sulphhate in the diet for cattle and 75 ppm of molybdenum and a similar level of sulphate in the diet for sheep. Several parameters of copper metabolism were studied in control and deficient animals by means of 67Cu and 64Cu. The plasma clearance of 67Cu showed that deficient animals removed the tracer at a slower rate than control animals, particularly in cattle. Normal cattle evidenced a higher rate of ceruloplasmin production than normal sheep, although the half-time of ceruloplasmin in the circulation of cattle seems to be shorter than in sheep. These differences in the kinetics of ceruloplasmin between cattle and sheep could explain the higher susceptibility of cattle to dietary molybdenum and sulphate. Apparent absorption of 67Cu from the gut of control and deficient sheep was similar, but the endogenous excretion of the injected tracer was higher in the deficient sheep. These results agree with previous data obtained with 64Cu, nevertheless further determinations are needed before drawing definitive conclusions on this aspect of copper metabolism. The intracellular distribution of 64Cu in the liver samples of control and deficient steers, as well as in normal sheep, showed differences among several fractions. The lack of previous data in normal animals, as well as the limited number of animals studied in the present work, do not allow a valid interpretation of these results. However, such data suggest that further studies in this field could explain the disturbances of copper metabolism in the liver demonstrated in previous research. (author)

  6. Influence of ferulic acid on γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes

    International Nuclear Information System (INIS)

    Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species (ROS) resulting in imbalance of the pro-oxidant and antioxidant activities ultimately resulting in cell death. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn, and ricebran. FA exhibit a wide range of pharmacological effects including antiageing, anti-inflammatory, anticancer, antidiabetic, antiapoptotic, and neuroprotective. The present work is aimed at evaluating the radioprotective effect of FA, on γ-radiation induced toxicity in primary cultures of isolated rat hepatocytes. Hepatocytes were isolated from the liver of rats by collagenase perfusion. The cellular changes were estimated using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS), the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH), ceruloplasmin, Vitamins A, E and C and uric acid. DNA damage was analyzed by single cell gel electrophoresis (comet assay). An increase in the severity of DNA damage was observed with increasing dose (1, 2 and 4 Gy) of γ-radiation in cultured hepatocytes. TBARS were increased significantly, whereas the levels of GSH, Vitamins C, E and A, ceruloplasmin, uric acid and antioxidant enzymes were significantly decreased in γ-irradiated groups. The maximum damage to hepatocytes was observed at 4 Gy irradiation. Pretreatment with FA (1, 5 and 10 μg/ml) significantly decrease the levels of TBARS and DNA damage. In addition, pretreatment with FA significantly increased antioxidant enzymes, GSH, Vitamins A, E and C, uric acid and ceruloplasmin levels. The maximum protection of hepatocytes was observed at 10 μg/ml of FA pretreatment. Thus, pretreatment with FA helps in protecting the hepatocytes against γ-radiation induced cellular damage and can be developed as a effective radioprotector during radiotherapy

  7. Electroencephalographic Fractal Dimension in Healthy Ageing and Alzheimer’s Disease

    Science.gov (United States)

    Cottone, Carlo; Cancelli, Andrea; Rossini, Paolo Maria; Tecchio, Franca

    2016-01-01

    Brain activity is complex; a reflection of its structural and functional organization. Among other measures of complexity, the fractal dimension is emerging as being sensitive to neuronal damage secondary to neurological and psychiatric diseases. Here, we calculated Higuchi’s fractal dimension (HFD) in resting-state eyes-closed electroencephalography (EEG) recordings from 41 healthy controls (age: 20–89 years) and 67 Alzheimer’s Disease (AD) patients (age: 50–88 years), to investigate whether HFD is sensitive to brain activity changes typical in healthy aging and in AD. Additionally, we considered whether AD-accelerating effects of the copper fraction not bound to ceruloplasmin (also called “free” copper) are reflected in HFD fluctuations. The HFD measure showed an inverted U-shaped relationship with age in healthy people (R2 = .575, p < .001). Onset of HFD decline appeared around the age of 60, and was most evident in central-parietal regions. In this region, HFD decreased with aging stronger in the right than in the left hemisphere (p = .006). AD patients demonstrated reduced HFD compared to age- and education-matched healthy controls, especially in temporal-occipital regions. This was associated with decreasing cognitive status as assessed by mini-mental state examination, and with higher levels of non-ceruloplasmin copper. Taken together, our findings show that resting-state EEG complexity increases from youth to maturity and declines in healthy, aging individuals. In AD, brain activity complexity is further reduced in correlation with cognitive impairment. In addition, elevated levels of non-ceruloplasmin copper appear to accelerate the reduction of neural activity complexity. Overall, HDF appears to be a proper indicator for monitoring EEG-derived brain activity complexity in healthy and pathological aging. PMID:26872349

  8. Electroencephalographic Fractal Dimension in Healthy Ageing and Alzheimer's Disease.

    Science.gov (United States)

    Smits, Fenne Margreeth; Porcaro, Camillo; Cottone, Carlo; Cancelli, Andrea; Rossini, Paolo Maria; Tecchio, Franca

    2016-01-01

    Brain activity is complex; a reflection of its structural and functional organization. Among other measures of complexity, the fractal dimension is emerging as being sensitive to neuronal damage secondary to neurological and psychiatric diseases. Here, we calculated Higuchi's fractal dimension (HFD) in resting-state eyes-closed electroencephalography (EEG) recordings from 41 healthy controls (age: 20-89 years) and 67 Alzheimer's Disease (AD) patients (age: 50-88 years), to investigate whether HFD is sensitive to brain activity changes typical in healthy aging and in AD. Additionally, we considered whether AD-accelerating effects of the copper fraction not bound to ceruloplasmin (also called "free" copper) are reflected in HFD fluctuations. The HFD measure showed an inverted U-shaped relationship with age in healthy people (R2 = .575, p < .001). Onset of HFD decline appeared around the age of 60, and was most evident in central-parietal regions. In this region, HFD decreased with aging stronger in the right than in the left hemisphere (p = .006). AD patients demonstrated reduced HFD compared to age- and education-matched healthy controls, especially in temporal-occipital regions. This was associated with decreasing cognitive status as assessed by mini-mental state examination, and with higher levels of non-ceruloplasmin copper. Taken together, our findings show that resting-state EEG complexity increases from youth to maturity and declines in healthy, aging individuals. In AD, brain activity complexity is further reduced in correlation with cognitive impairment. In addition, elevated levels of non-ceruloplasmin copper appear to accelerate the reduction of neural activity complexity. Overall, HDF appears to be a proper indicator for monitoring EEG-derived brain activity complexity in healthy and pathological aging. PMID:26872349

  9. Copper binding components of blood plasma and organs, and their responses to influx of large doses of 65Cu, in the mouse

    OpenAIRE

    Cabrera, Anthony; Alonzo, Erin; Sauble, Eric; Chu, Yu Ling; MARIA C LINDER; Sato, Dee S.; Mason, Andrew Z.

    2008-01-01

    To establish for the first time how mice might differ from rats and humans in terms of copper transport, excretion, and copper binding proteins, plasma and organ cytosols from adult female C57CL6 mice were fractionated and analyzed by directly coupled size exclusion HPLC-ICP-MS, before and after i.p. injection of large doses of 65Cu. Plasma from untreated mice had different proportions of Cu associated with transcuprein/macroglobulin, ceruloplasmin and albumin than in humans and rats, and two...

  10. Screening for Wilson disease in acute liver failure: a comparison of currently available diagnostic tests

    DEFF Research Database (Denmark)

    Korman, J.D.; Volenberg, I.; Balko, J.;

    2008-01-01

    Acute liver failure (ALF) due to Wilson disease (WD) is invariably fatal without emergency liver transplantation. Therefore, rapid diagnosis of WD should aid prompt transplant listing. To identify the best method for diagnosis of ALF due to WD (ALF-WD), data and serum were collected from 140 ALF...... patients (16 with WD), 29 with other chronic liver diseases and 17 with treated chronic WD. Ceruloplasmin (Cp) was measured by both oxidase activity and nephelometry and serum copper levels by atomic absorption spectroscopy. In patients with ALF, a serum Cp <20 mg/dL by the oxidase method provided a...

  11. A combination of serum iron, ferritin and transferrin predicts outcome in patients with intracerebral hemorrhage

    OpenAIRE

    Guang Yang; Rong Hu; Chao Zhang; Christopher Qian; Qian-Qian Luo; Wing-Ho Yung; Ya Ke; Hua Feng; Zhong-Ming Qian

    2016-01-01

    Association of a high-serum ferritin with poor outcome showed that iron might play a detrimental role in the brain after intracerebral hemorrhage (ICH). Here, we investigated changes in serum iron, ferritin, transferrin (Tf) and ceruloplasmin (CP) in patients with ICH (n = 100) at day 1 (admission), 3, 7, 14 and 21 and those in control subjects (n = 75). The hematoma and edema volumes were also determined in ICH-patients on admission and at day 3. The Modified Rankin Scale (mRS) of 59 patient...

  12. Increased Copper-Mediated Oxidation of Membrane Phosphatidylethanolamine in Autism

    Directory of Open Access Journals (Sweden)

    Abha Chauhan

    2008-01-01

    Full Text Available We have previously reported that levels of phosphatidylethanolamine (PE in the erythrocyte membrane and of ceruloplasmin, a copper-transport antioxidant protein, in the serum are lower in children with autism than in control subjects. In the present study, we report that (a copper oxidizes and reduces the levels of membrane PE and (b copper-mediated oxidation of PE is higher in lymphoblasts from autistic subjects than from control subjects. The effect of copper was examined on the oxidation of liposomes composed of brain lipids from mice and also on the lymphoblasts from autism and control subjects. Among the various metal cations (copper, iron, calcium, cadmium and zinc, only copper was found to oxidize and decrease the levels of PE. The metal cations did not affect the levels of other phospholipids. The action of copper on PE oxidation was time-dependent and concentration-dependent. No difference was observed between copper-mediated oxidation of diacyl-PE and alkenyl-PE (plasmalogen, suggesting that plasmalogenic and non-plasmalogenic PE are equally oxidized by copper. Together, these studies suggest that ceruloplasmin and copper may contribute to oxidative stress and to reduced levels of membrane PE in autism.

  13. ACUTE PHASE REACTANCTS IN PERICARDIAL FLUID ARE INDICATORS OF CORONARY ARTERY DISEASE

    Directory of Open Access Journals (Sweden)

    Yılmaz Mehmet Ali

    2012-12-01

    Full Text Available Inflammation in formation of atherosclerosis, and acute phase reactants in the site of inflammation have major functions. Thus, do the acute phase reactants constitute the biggest risk factor for coronary artery disease? 55 patients are included in the study. Patients with coronary artery bypass surgery are included in Group I (38 patients and patients with valve operation are included in Group II (17 patients. CABG patients are further divided into two sub-groups as on-pump and off-pump. In both groups, homocystein, high sensitivity C reactive protein, ceruloplasmin, lipoprotein A and serum amyloid A protein levels are analyzed from blood and pericardial fluid. In patients with coronary artery disease, the measured high specific C- reactive protein levels from blood and pericardial fluid are found to be significantly high compared to patients with valve operation.Homocystein levels of pericardial fluids of patients with CABG are found to be higher than patients with valve operation and it is confirmed that the situation is correlated with blood homocystein levels. Although there are lots studies expressing the relation between coronary artery disease and lipoprotein A, ceruloplasmin and serum amyloid A protein levels; no significant difference for those parameters was obtained in our study. We determined that other phase reactants are higher in patients with coronary artery disease, in accordance with the literature. We aimed to state that acute phase reactants not only increase as a result of disease, but their levels are also elevated beforehand, as an indicator of the disease.

  14. Experimental caprine coccidiosis: the pattern of changes in antioxidant micronutrients and vitamins

    Directory of Open Access Journals (Sweden)

    Ehsan Rakhshandehroo

    2014-09-01

    Full Text Available This study aimed at investigating the status of non-enzymatic antioxidant agents during experimental caprine coccidiosis. A total of 20 newborn kids were selected and allocated into 2 (diseased and healthy groups. Ten of the kids were infected with sporulated oocysts of the most pathogenic species of Eimeria and ten served as controls. Blood samples were taken at 0 (before inoculation, 3, 7, 14, 21, 28 and 35 days post infection (dpi and the concentrations of antioxidant trace elements, antioxidant vitamins and ceruloplasmin were measured. Our data showed remarkable reductions in serum concentrations of some antioxidant trace elements (zinc, manganese and selenium and vitamins (vitamin C in the diseased kids, however, significant increases were observed in the serum level of ceruloplasmin in infected animals. These alterations became more prominent at 14 to 21 dpi. These observations suggest that Eimeria parasites can significantly interfere with the levels of some antioxidant trace elements and vitamins during caprine coccidiosis. These changes indicate the overproduction of oxidative radicals during the pathogenesis of Eimeria species that may account for extensive oxidative damage in infected animals.

  15. Serum Trace Element Concentrations in Rheumatoid Arthritis.

    Science.gov (United States)

    Sahebari, Maryam; Ayati, Razie; Mirzaei, Hamed; Sahebkar, Amirhossein; Hejazi, Sepideh; Saghafi, Massoud; Saadati, Nayyereh; Ferns, Gordon A; Ghayour-Mobarhan, Majid

    2016-06-01

    Rheumatoid arthritis (RA) is a condition that is associated with oxidative stress. Serum trace elements and their related transport proteins, e.g., albumin and ceruloplasmin, play an important role in the antioxidant defense. Trace element status may therefore be involved in the pathogenesis of RA or be affected by the disease activity of this chronic inflammatory condition. The study participants were 110 patients with RA and 100 sex- and age-matched healthy volunteers. Serum concentrations of albumin, ceruloplasmin, selenium, zinc, copper, and zinc/copper ratio were measured in all subjects. The relationship between these parameters and disease activity score was also assessed. Lower concentrations of serum Alb, Zn, and Se were independently related to disease activity index. High concentrations of serum copper were associated with the presence of RA. Serum Cu concentrations were positively related to disease activity as assessed by the disease activity score. Low serum concentrations of Zn and Se, and high serum Cu concentrations may be associated with the presence of RA or be a consequence of this condition. Of the trace elements that were investigated in the present study, only serum Cu was positively correlated with disease activity. PMID:26450515

  16. Abnormal copper metabolism in Menke's steely-hair syndrome.

    Science.gov (United States)

    Lott, I T; Dipaolo, R; Raghavan, S S; Clopath, P; Milunsky, A; Robertson, W C; kanfer, J N

    1979-07-01

    Copper (Cu) metabolism was selectively studied in seven infants with Menke's steely-hair syndrome (SHS). A daily oral regimen of CuSO4 (584 microgram Cu/kg) and L-histidine (100 mg/kg) in three infants produced an increase in serum Cu concentrations ranging from 33-95% of normal, but without the formation of ceruloplasmin. Cohn serum protein fractionation after oral Cu/L-histidine loading showed a disproportionate accumulation of Cu in the albumin fraction (V). The electron spin resonance spectrum of fraction V showed a heightened signal for the SHS patients, suggesting that an increased concentration of a radical Cu species is present after oral loading. The Sephadex G-150 chromatographic profile of serum fraction V in SHS did not differ significantly from controls. These results suggest that, in SHS, Cu absorbed in the presence of L-histidine is in an abnormal complex involving albumin, which does not allow for holoceruloplasmin biosynthesis. Cu and ceruloplasmin concentrations in the cord blood specimen of an infant who went on to develop SHS were normal, a finding which may account for the transient period of seemingly normal development after birth in SHS patients. An almost 6-fold difference in mean Cu concentration was observed in SHS fibroblasts compared to controls. Fibroblast Cu concentration was elevated in one to two possible maternal heterozygotes, a finding which may permit diagnosis of the carrier state for some SHS heterozygotes. PMID:481958

  17. Cp/Heph mutant mice have iron-induced neurodegeneration diminished by deferiprone.

    Science.gov (United States)

    Zhao, Liangliang; Hadziahmetovic, Majda; Wang, Chenguang; Xu, Xueying; Song, Ying; Jinnah, H A; Wodzinska, Jolanta; Iacovelli, Jared; Wolkow, Natalie; Krajacic, Predrag; Weissberger, Alyssa Cwanger; Connelly, John; Spino, Michael; Lee, Michael K; Connor, James; Giasson, Benoit; Harris, Z Leah; Dunaief, Joshua L

    2015-12-01

    Brain iron accumulates in several neurodegenerative diseases and can cause oxidative damage, but mechanisms of brain iron homeostasis are incompletely understood. Patients with mutations in the cellular iron-exporting ferroxidase ceruloplasmin (Cp) have brain iron accumulation causing neurodegeneration. Here, we assessed the brains of mice with combined mutation of Cp and its homolog hephaestin. Compared to single mutants, brain iron accumulation was accelerated in double mutants in the cerebellum, substantia nigra, and hippocampus. Iron accumulated within glia, while neurons were iron deficient. There was loss of both neurons and glia. Mice developed ataxia and tremor, and most died by 9 months. Treatment with the oral iron chelator deferiprone diminished brain iron levels, protected against neuron loss, and extended lifespan. Ferroxidases play important, partially overlapping roles in brain iron homeostasis by facilitating iron export from glia, making iron available to neurons. Above: Iron (Fe) normally moves from capillaries to glia to neurons. It is exported from the glia by ferroportin (Fpn) with ferroxidases ceruloplasmin (Cp) and/or Hephaestin (Heph). Below: In mice with mutation of Cp and Heph, iron accumulates in glia, while neurons have low iron levels. Both neurons and glia degenerate and mice become ataxic unless given an iron chelator. PMID:26303407

  18. Role of hepcidin and its downstream proteins in early brain injury after experimental subarachnoid hemorrhage in rats.

    Science.gov (United States)

    Tan, Guanping; Liu, Liu; He, Zhaohui; Sun, Jiujun; Xing, Wenli; Sun, Xiaochuan

    2016-07-01

    Early brain injury (EBI) is a major cause of mortality from subarachnoid hemorrhage (SAH). We aimed to study the pathophysiology of EBI and explore the role of hepcidin, a protein involved in iron homeostatic regulation, and its downstream proteins. One hundred and thirty-two male Sprague-Dawley rats were assigned into groups (n = 24/group): sham, SAH, SAH + hepcidin, SAH + hepcidin-targeting small interfering ribonucleic acid (siRNA), and SAH + scramble siRNA. Three hepcidin-targeting siRNAs and one scramble siRNA for hepcidin were injected 24 h before hemorrhage induction, and hepcidin protein was injected 30 min before induction. The rats were neurologically evaluated at 24 h and euthanized at 72 h. Hepcidin, ferroportin-1, and ceruloplasmin protein expression were measured by immunohistochemistry and Western blotting. Brain water content, blood-brain barrier (BBB) leakage, non-heme tissue iron and Garcia scale were evaluated. Hepcidin expression increased in the cerebral cortex and hippocampus after experimental SAH (P Hepcidin injection lowered the expression of ferroportin-1 and ceruloplasmin further but siRNA reduced the levels of hepcidin (P hepcidin, but decreased by siRNA (P hepcidin enhanced iron-dependent oxidative damage and may be the potential mechanism of SAH. PMID:27250827

  19. Protection of DNA strand breakage by radiation exposure

    International Nuclear Information System (INIS)

    Human ceruloplasmin, the plasma copper containing protein, is thought to play an essential role in iron metabolism, but it also has antioxidant properties. Ceruloplasmin directly scavenged hydroxyl radicals (.OH) generated in dithiothreitol/FeCl3 system besides inhibitory function of hydroxyl radical formation and lipid peroxidation. Polyamines, spermidine and spermine, significantly protected the supercoiled DNA strand breakage by hydroxyl radicals and DNA strand breakage by UV was highly protected by all four polyamines used in this study. In polyamine deficient mutant KL527. It was shown that cell survivability following UV irradiation was slightly increased by exogenous polyamines putrescine and spermidine supplement. However the cell survivability of wild type (MG 1655) was not influenced by polyamine supplement. In γ-irradiated cells, cell survivability of polyamine-deficient mutant strain KL527 was significantly increased by exogenous putrescine supplement and that of wild type strain MG1655 was similar irrespective of polyamine supplement. These results implicate the possibility that polyamines play a potent role in radioprotection of cell and DNA level. (author). 32 refs., 8 figs

  20. In-vitro use of radiominerals to determine mineral imbalances in farm animals. Part of a coordinated programme on the use of isotopes to diagnose moderate mineral imbalances in farm animals

    International Nuclear Information System (INIS)

    This research programme involved the use of in vitro radioisotopic procedures as well as stable element analysis and enzyme determinations to evaluate selenium, copper and zinc deficiency states in sheep in Turkey. Uptake of radioactive selenium by red blood cells was effective in determining the selenium status of sheep. Animals on a selenium deficient diet had red cell selenium uptakes significantly greater than those of sheep fed normal levels of selenium. When sheep on a selenium deficient diet were tested and then supplemented with selenium for several weeks, the red cell uptakes of radioselenium after supplementation were significantly less than before supplementation. A similar technique was used to study the zinc status of sheep. Uptake of radioactive zinc by red cells was greater in deficient sheep than in zinc-supplemented animals. Zinc deficient sheep also had lower serum zinc levels than in supplemented animals, and alkaline phosphatase activity levels. Assays of erythrocyte uptake, using a scintillation counter may be useful in the absence of equipment for total mineral analysis or enzyme assays. In another study the serum copper levels of sheep of different genetic types were compared to serum ceruloplasmin levels and to the incidence of enzootic ataxia in offspring of these animals. A definite correlation was found between haemoglobulin type, serum copper level and ceruloplasmin level. Comparisons of these parameters in sheep with copper and molybdenum levels in forage samples indicated that copper deficiency in sheep in Turkey is apparently a secondary copper deficiency due to high levels of molybdenum in forage

  1. Protection of DNA strand breakage by radiation exposure

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jeong Ho; Kim, In Gyu; Lee, Kang Suk; Kim, Kug Chan; Shim, Hae Won

    1997-12-01

    Human ceruloplasmin, the plasma copper containing protein, is thought to play an essential role in iron metabolism, but it also has antioxidant properties. Ceruloplasmin directly scavenged hydroxyl radicals (.OH) generated in dithiothreitol/FeCl{sub 3} system besides inhibitory function of hydroxyl radical formation and lipid peroxidation. Polyamines, spermidine and spermine, significantly protected the supercoiled DNA strand breakage by hydroxyl radicals and DNA strand breakage by UV was highly protected by all four polyamines used in this study. In polyamine deficient mutant KL527. It was shown that cell survivability following UV irradiation was slightly increased by exogenous polyamines putrescine and spermidine supplement. However the cell survivability of wild type (MG 1655) was not influenced by polyamine supplement. In {gamma}-irradiated cells, cell survivability of polyamine-deficient mutant strain KL527 was significantly increased by exogenous putrescine supplement and that of wild type strain MG1655 was similar irrespective of polyamine supplement. These results implicate the possibility that polyamines play a potent role in radioprotection of cell and DNA level. (author). 32 refs., 8 figs

  2. Serum ferritin and stomach cancer risk among A-bomb survivors

    International Nuclear Information System (INIS)

    Using stored serum samples collected from 1970-72 and/or from 1977-79, serum ferritin, transferrin, and ceruloplasmin levels were immunologically determined for 233 stomach cancer and 84 lung cancer cases diagnosed from 1973-83 and for 385 matched controls from a fixed population of Hiroshima and Nagasaki atomic bomb survivors. Elevated stomach cancer risk was associated with low serum ferritin levels, with more than a threefold excess among those in the lowest quintile as compared to the highest ferritin quintile. The average serum ferritin concentration was 8% lower in the stomach cancer cases than in the controls. Risk did not vary with the time between blood collection and stomach cancer onset, remaining high among those with low ferritin levels five or more years before cancer diagnosis. Low ferritin combined with achlorhydria, diagnosed about 10 years before the blood collection and up to 25 years before cancer diagnosis, was an exceptionally strong marker of increased stomach cancer risk. No effect of transferrin or ceruloplasmin independent of ferritin was observed on gastric cancer risk. Lung cancer risk was not related to these three serum proteins. (author)

  3. A study on the radiation and environment safety -Development of technology for biological dosimetry-

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kang Suk; Kim, Kook Chan; Kim, In Kyoo; Kim, Jin Kyoo; Chun Kee Jung; Park, Hyo Kook; Kim, Sang Bok; Park Sun Yung [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-07-01

    Adult rats were treated a single, whole body exposure to a dose of 0.1, 0.5, 1.0, 2.0, 3.0 Gy. The animals were sacrificed 6, 24, 48, 72, 96 hours following exposure. The amount of serum acute phase proteins(haptoglobin, ceruloplasmin, C-reactive protein, alpha-1 antitrypsin, alpha-1 acid glycoprotein, transferrin) were measured by competitive ELISA. In the 0.1 Gy irradiated rats, serum haptoglobin, C-reactive protein and alpha-1 antitrypsin were 400% higher and serum transferrin was 50% lower as compared to controls, 96 hours after irradiation. Ceruloplasmin increased by 400%, 24 hours after irradiation, but 96 hours after irradiation, the concentration of this protein in rat returned to normal level. On the other hand, no changes were observed in the case of alpha-1 acid glycoprotein. In the group of the 3.0 Gy irradiated rats, transferrin increased by 200%, 96 hours after irradiation. These biochemical responses to radiation did not show dose-dependent relation, but the sensitivity of the indicators was high enough to detect absorbed dose of 0.1 Gy. The above results can be applied to the measurements of acute phase reactants in human serum for the assessment of exposure doses in radiation workers and patients under radiation therapy. 39 figs, 72 refs. (Author).

  4. [Copper deficiency with pancytopenia, bradycardia and neurologic symptoms].

    Science.gov (United States)

    Miki, Hirokazu; Kuwayama, Yasuharu; Hara, Tomoko; Oaki, Keiji; Kanezaki, Yoshiko; Yoshida, Tomonori; Shintani, Yasumi; Miya, Keiko; Goto, Tetsuya

    2007-03-01

    A 48-year-old man was referred to our hospital in December, 2005 because of general fatigue, gait disturbance and bradycardia. He had a history of polysurgery due to recurrent ileus and had been treated with home total parenteral nutrition for the short-bowel syndrome since 2003. Clinical findings on admission included marked emaciation and severe weakness of the extremities. Pancytopenia was noted in the peripheral blood. The serum levels of copper and ceruloplasmin were 3 microg/dl and 3 mg/dl, respectively, while Vit. B12 and folate were within the normal range. The bone marrow demonstrated cytoplasmic vacuolation in the myeloid and megakaryocytic series, and sideroblastic changes. No evidence of hematologic malignancies was presented. The diagnosis was copper deficiency and the patient was treated with copper supplementation. Four weeks after copper therapy, the serum level of copper rose to 50 microg/dl and ceruloplasmin to 14 mg/dl. Significant improvements in the hematologic profile, ECG findings and weakness of extremities were noted. Although bicytopenia (anemia and neutropenia) is considered to be a feature of hematologic disorders caused by copper deficiency, the present case showed pancytopenia. The exact mechanism of the unusual association of thrombocytopenia and other abnormalities with copper deficiency remains to be elucidated. PMID:17441478

  5. Studies of Internal Environment of Psoriasis and Its Therapy

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@  Psoriasis is a kind of refractory disease, though it is less serious than cancer. The etiology is not clear, the morbidity is high, and it cannot be radically cured. It is an urgent problem that needs to be solved. Since 1980, the authors have studied the pathogenic mechanisms of psoriasis from aspects of pathology, biochemistry, pharmacology, and immunology. It was proved that abnormalities in biochemical components, neural media, immunological state and cell metabolism existed in psoriatic cases. We have published more than ten articles, and formulated two anti-psoriasis drugs, which is reported as follows:   1. The levels of serum zinc, copper, calcium, magnesium, iron, selenium, ceruloplasmin, vitamin A and vitamin E in 138 psoriatic patients were measured by atomic absorption spectrometry. The results showed that serum copper level in the progressive and stable stage was decreased significantly (P<0.01). The levels of serum calcium and vitamin E lowered too. The levels of serum ceruloplasmin, selenium and iron were considerably higher in psoriatic than those in the normal control. This is an evidence that there are biochemical abnormalities in the internal environment of psoriatic patients.

  6. Studies of Internal Environment of Psoriasis and Its Therapy

    Institute of Scientific and Technical Information of China (English)

    XU; Han-qing

    2001-01-01

    Psoriasis is a kind of refractory disease, though it is less serious than cancer. The etiology is not clear, the morbidity is high, and it cannot be radically cured. It is an urgent problem that needs to be solved. Since 1980, the authors have studied the pathogenic mechanisms of psoriasis from aspects of pathology, biochemistry, pharmacology, and immunology. It was proved that abnormalities in biochemical components, neural media, immunological state and cell metabolism existed in psoriatic cases. We have published more than ten articles, and formulated two anti-psoriasis drugs, which is reported as follows:    1. The levels of serum zinc, copper, calcium, magnesium, iron, selenium, ceruloplasmin, vitamin A and vitamin E in 138 psoriatic patients were measured by atomic absorption spectrometry. The results showed that serum copper level in the progressive and stable stage was decreased significantly (P<0.01). The levels of serum calcium and vitamin E lowered too. The levels of serum ceruloplasmin, selenium and iron were considerably higher in psoriatic than those in the normal control. This is an evidence that there are biochemical abnormalities in the internal environment of psoriatic patients.……

  7. Genetic effects on acute phase protein response to the stresses of weaning and transportation in beef calves.

    Science.gov (United States)

    Qiu, X; Arthington, J D; Riley, D G; Chase, C C; Phillips, W A; Coleman, S W; Olson, T A

    2007-10-01

    The objective herein was to estimate heterosis and breed effects in purebred and crossbred Romosinuano, Brahman, and Angus calves on acute phase protein response to weaning and transportation. Calves (n = 1,032) were weaned in September of 2002, 2003, and 2004 at approximately 7 mo of age. Approximately 28 d after weaning, steer calves (n = 482) were transported 1,800 km (20 h) to Oklahoma. Concentrations of 3 acute phase proteins (ceruloplasmin, fibrinogen, and haptoglobin) were measured in blood samples. Calves (steers and heifers) were sampled at weaning, and 24 and 72 h postweaning. For separate analyses, steers sent to Oklahoma were sampled before shipment, upon arrival, and 24 and 72 h after arrival. Combinations of the following fixed effects were investigated: sire breed, dam breed, sampling time, birth location, calf sex (weaning only), year, cow age, and interactions. Effects of special interest were sire breed x dam breed as an indication of breed group of calf, and the interaction of sire and dam breeds with sampling time. Weaning age and BW were investigated as linear and quadratic covariates. Sire of calf within sire breed was a random term. The correlation structure of repeated measures was determined by comparison of information criterion values for different structures within each analysis. In general, plasma acute phase protein concentrations in weaned calves increased with sampling time. Concentrations in the transported steers increased through sampling at 24 h after arrival, and were lower at 72 h. Significant estimates of heterosis were detected for Brahman-Angus haptoglobin concentrations at weaning (0.38 +/- 0.14 mg/dL x 100; 44%), and for Romosinuano-Angus fibrinogen concentrations at weaning (11.4 +/- 5.5 mg/dL; 10%) and in transported steers (22.5 +/- 8.4 mg/dL; 20%). The direct effect of Romosinuano was to increase (P Angus was to lower ceruloplasmin concentrations in weaned calves (-3.9 +/- 1.2; P = 0.001). Significant maternal effects

  8. Clinical perspective on C-reactive protein in prognostication of major adverse cardiac events in the elderly with established coronary heart disease

    Institute of Scientific and Technical Information of China (English)

    Olabode Oladeinde

    2006-01-01

    @@ The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).

  9. Alcoholic Stem Extract of Coscinium fenestratum Regulates Carbohydrate Metabolism and Improves Antioxidant Status in Streptozotocin–Nicotinamide Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    I. R. Punitha

    2005-01-01

    Full Text Available Alcoholic extract of the stems of Coscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin–nicotinamide induced type 2 diabetic rats. Oral administration of C. fenestratum stem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity of C. fenestratum.

  10. Wilson's disease: start with psychiatric symptoms. Brain magnetic resonance findings

    International Nuclear Information System (INIS)

    Wilson's disease - hepatolenticular degeneration - is an autosomal recessive genetic disorder, characterized by an excessive and toxic accumulation of cooper in different tissues. This accumulation is produced by an inherited defect in cooper's biliary excretion. This rare disorder affects approximately one on 30.000 individuals. Signs and symptoms of hepatic, neurologic and psychiatric disease are the most common clinical presentations of symptomatic Wilson's disease. The diagnosis can usually be made by laboratory tests that find a decreased cooper binding protein in blood called ceruloplasmin, an increase in the excretion of cooper in 24 hour urine and the appearance of corneal Kayser-Fleischer ring. We present a 28 years patient who began with depression and panic attacks, followed by neurologic symptoms. Brain MRI was performed and showed different alterations suggesting the diagnosis of this infrequent sickness. (author)

  11. Good response with zinc acetate monotherapy in an adolescent affected by severe Wilson disease

    Directory of Open Access Journals (Sweden)

    M.G. Marazzi

    2012-04-01

    Full Text Available We describe a 17-year-old girl with haemolytic anaemia as presentation of Wilson disease. The diagnosis was based on the findings of < 20 mg/dl ceruloplasmin serum level, Kayser-Fleischer ring and Coombs-negative haemolytic anaemia. Genetic testing revealed the presence of the H1069Q heterozygous mutation. The patient was treated with Zinc acetate monotherapy, with good response, maintened after 22 months. This case emphasizes the importance of recognizing atypical clinical presentation of Wilson disease, which must always be considered in patients with Coombs-negative haemolytic anaemia. The good clinical response to treatment with zinc acetate monotherapy in our case might lend to consider the use of zinc monotherapy as initial therapy also in symptomatic patients with Wilson disease under close clinical observation. Clinical trials are needed to provide evidence for use of zinc monotherapy as first-line therapy in symptomatic patients with Wilson disease.

  12. Deregulation of proteins involved in iron metabolism in hepcidin-deficient mice.

    Science.gov (United States)

    Viatte, Lydie; Lesbordes-Brion, Jeanne-Claire; Lou, Dan-Qing; Bennoun, Myriam; Nicolas, Gaël; Kahn, Axel; Canonne-Hergaux, François; Vaulont, Sophie

    2005-06-15

    Evidence is accumulating that hepcidin, a liver regulatory peptide, could be the common pathogenetic denominator of all forms of iron overload syndromes including HFE-related hemochromatosis, the most prevalent genetic disorder characterized by inappropriate iron absorption. To understand the mechanisms whereby hepcidin controls iron homeostasis in vivo, we have analyzed the level of iron-related proteins by Western blot and immunohistochemistry in hepcidin-deficient mice, a mouse model of severe hemochromatosis. These mice showed important increased levels of duodenal cytochrome b (Dcytb), divalent metal transporter 1 (DMT1), and ferroportin compared with control mice. Interestingly, the level of ferroportin was coordinately up-regulated in the duodenum, the spleen, and the liver (predominantly in the Kupffer cells). Finally, we also evidenced a decrease of ceruloplasmin in the liver of hepcidin-deficient mice. We hypothesized that the deregulation of these proteins might be central in the pathogenesis of iron overload, providing key therapeutic targets for iron disorders. PMID:15713792

  13. A review on laboratory liver function tests

    Directory of Open Access Journals (Sweden)

    Shruthi Kulkarni

    2009-11-01

    Full Text Available Laboratory liver tests are broadly defined as tests useful in the evaluation and treatment of patients with hepatic dysfunction. The liver carries out metabolism of carbohydrate, protein and fats. Some of the enzymes and the end products of the metabolic pathway which are very sensitive for the abnormality occurred may be considered as biochemical marker of liver dysfunction. Some of the biochemical markers such as serum bilirubin, alanine amino transferase, aspartate amino transferase, ratio of aminotransferases, alkaline phosphatase, gamma glutamyl transferase, 5´ nucleotidase, ceruloplasmin, alpha-fetoprotein are considered in this article. An isolated or conjugated alteration of biochemical markers of liver damage in patients can challenge the clinicians during the diagnosis of disease related to liver directly or with some other organs. The term “liver chemistry tests” is a frequently used but poorly defined phrase that encompasses the numerous serum chemistries that can be assayed to assess hepatic function and/or injury.

  14. Application of Glow Discharge Aes for Investigation of Metal Ions and Water in Biology and Medicine

    CERN Document Server

    Bregadze, Vasil G; Tsakadze, Ketevan J

    2007-01-01

    AES VHF inductively coupled plasmatron may be applied to wide range of studies. It enables rapid microanalysis of various solutions including biological objects and peripheral blood serum. In addition, it may be used for investigation of water desorption from solid bodies and for determination of energetic metal-macromolecule complexes. Study of hydration energy and hydration number by kinetic curves of water glow discharge atomic spectral analysis of hydrogen (GD EAS analysis of hydrogen) desorption from Na-DNA humidified fibers allowed to reveal that structural and conformational changes in activation energy of hydrated water molecules increases by 0.65kcal/Mole of water. The developed method of analysis of elements in solutions containing high concentrations of organic materials allows systematic study of practically healthy persons and reveals risk factors for several diseases. Microelemental content of blood serum fractions showed that amount of not bounded with ceruloplasmin copper was three times more ...

  15. Aceruloplasminaemia: a rare but important cause of iron overload.

    Science.gov (United States)

    Doyle, Adam; Rusli, Ferry; Bhathal, Prithi

    2015-01-01

    We present a case of a 20-year-old man referred to our service with iron overload and mildly deranged liver biochemistry. Although liver histopathology was consistent with haemochromatosis, iron studies were not consistent with this diagnosis. Serum ceruloplasmin levels were undetectable, leading to a diagnosis of aceruloplasminaemia. Unlike other iron overload disorders, neurological complications are a unique feature of this illness, and often irreversible, once established. The patient was treated with iron chelation prior to the onset of neurological injury, and experienced progressive normalisation of his ferritin and liver biochemistry. This is one of the youngest diagnosed cases in the published literature and, crucially, was a rare case of diagnosis and treatment prior to the onset of neurological sequelae. This is presented alongside a review of previously published cases of aceruloplasminaemia, including responses to iron chelation therapy. PMID:25976187

  16. Different dietary lifestyles and serum zinc and copper in women of reproductive age

    Energy Technology Data Exchange (ETDEWEB)

    Breskin, M.W.; Worthington-Roberts, B.S.; Monsen, E.R.

    1986-03-01

    Nutrient intakes and biochemical measures of zinc and copper were compared in non-pregnant young women representing different dietary lifestyles, viz, those who habitually ate red meat (RM), fish or poultry (FP), or lacto-ovo-vegetarian (LV) sources of protein. All were in good health and concerned about their diets; none was using supplements or oral contraceptives. Three-day food records were analyzed for nutrient content. Serum and drinking water samples were assayed for zinc and copper (AAS), and serum, for ceruloplasmin (RID). Sign. diff. in dietary Zn or Cu content were not reflected by serum(Zn) or (Cu), but the incidence of serum(Zn) < the 95% CI for RM eaters was sign. higher in FP and LV groups (X/sup 2/ = 20.65, p < 0.001). Thus, use of diets limited in animal protein sources may be associated with an increase risk of low serum (Zn).

  17. Acute phase protein response in Alpine ibex with sarcoptic mange.

    Science.gov (United States)

    Rahman, Md Mizanur; Lecchi, Cristina; Fraquelli, Cristina; Sartorelli, Paola; Ceciliani, Fabrizio

    2010-03-25

    The acute phase proteins (APP) are a group of serum proteins that change their concentration in animals following external or internal challenges, such as infection, inflammation or stress. The concentrations of four APPs, including serum amyloid A (SAA), haptoglobin (Hp), alpha(1)-acid glycoprotein (AGP) and ceruloplasmin (Cp) were determined in serum collected from healthy Alpine ibexes (Capra ibex) and ibexes with Sarcoptes scabiei mange. Primary structures of all four APPs were determined by cDNA sequencing. The concentrations of all four APPs were higher in serum of animals with clinical signs of sarcoptic mange when compared to healthy animals. Two of the APPs, including SAA and AGP, acted as major APPs, since their serum concentrations were increased more than 10-folds when compared to healthy animals (Psarcoptic mange in free ranging animals. PMID:20036058

  18. Dithiothreitol inhibits the acceleration of DNA strand breaks by human serum albumin in a metal-catalyzed oxidation and γ-irradiation system

    International Nuclear Information System (INIS)

    Intact or Cys-34 modified human serum albumin (HSA) was significantly damaged and degraded in the metal-catalyzed oxidation (MCO) system containing dithiothreitol (DTT) as an electron donor and, while it lasted, this protein might have prohibited the hydroxy radical OH or H2O2 from attacking DNA. However, in the glutathione (GSH) and ascorbate (non-thiol) MCO system, HSA was not sacrificially degraded and accelerated rather than prevented DNA strand breaks. In contrast, human ceruloplasmin was not sacrificially degraded but significantly prevented DNA strand breaks in both GSH and DTT MCO system. In γ-irradiation system, only DTT attenuated the acceleration of DNA strand breaks by HSA. This protein did not remove more H2O2 in the presence of reduced GSH (thiol-linked peroxidase) than in the absence of GSH. Therefore it seems that in an MCO system, the antioxidant activity of HSA is dependent on the effectiveness of reducing equivalents to induce the exposure of a functional group scavenging hydroxy radical OH or H2O2 by reduction of disulfide bonds in HSA. In the presence of DTT, disulfide bonds in HSA were completely reduced to cysteinyl residues but not significantly converted into cysteines by ascorbate or GSH. On the other hand, human ceruloplasmin has a resistance to reduction. In conclusion, the antioxidant activity of HSA in the DTT MCO system is thought not to be due to peroxidase function by thiol specificity, but due to sacrificial antioxidant activity by the functional groups, such as hydrophobic compounds exposed to the protein surface by DTT. (author)

  19. Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males

    International Nuclear Information System (INIS)

    Objectives: To evaluate the efficacy of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 serum levels and improving antioxidant function in young healthy males during exhaustive exercise. Methods: The study was conducted at Ardabil University of Medical Sciences, Iran, in 2010-11 and comprised 28 healthy subjects in 18-24 age bracket. They were randomly divided into control and supplemented groups. One cup of pomegranate juice and one cup of tap water were given to supplemented and control groups daily for two weeks respectively. Fasting blood samples were taken at baseline and at the end of two weeks of intervention. The subjects were given one exhaustive exercise and then fasting blood samples were taken for testing blood glutathione peroxidase and superoxide dismutase and serum levels of high sensitivity C-reactive protein, zinc, ceruloplasmin, matrix metalloproteinases 2 and 9, malondialdehyde and total antioxidant capacity. Data was analysed using descriptive statistical tests, paired and independent sample t-test. Results: The blood levels of glutathione peroxidase and superoxide dismutase and serum levels of total antioxidant capacity after exhaustive exercise in the supplemented group were significantly increased (p<0.05), while the content of matrix metalloproteinases 2 and 9, ceruloplasmin and malondialdehyde showed a significant decrease in comparison to the control group (p<0.05). Besides, there were no significant changes in other biochemical factors. Conclusion: Regular intake of pomegranate juice significantly modulates matrix metalloproteinases 2 and 9 serum levels of some inflammatory factors and thus protects against exhaustive exercise-induced oxidative injury in young healthy males. (author)

  20. The influence of homocysteine and oxidative stress on pregnancy outcome.

    Science.gov (United States)

    Micle, O; Muresan, M; Antal, L; Bodog, F; Bodog, A

    2012-02-22

    Oxidative stress in utero-placental tissues plays an important role in the development of placental-related diseases. Maternal hiperhomocysteinemia is associated with placental mediated diseases, such as preeclampsia, spontaneous abortion and placental abruption. The aim of our study is to appreciate the clinical usefulness of the dosage serum homocysteine and malondialdehyde, as an oxidative stress marker, in the pregnancies complicated with risk of abortion or preterm birth. The study was performed at the Obstetric Gynecology Clinical Hospital Oradea from December 2009 until April 2010. It included 18 patients with risk of abortion (group 1), 22 with preterm birth (group 2). The results were compared with a control group composed by 14 healthy pregnant women. Serum homocysteine level was measured by an enzymatic method, on the instrument Hitachi 912, Roche, reagent: Axis-Shield Enzymatic. For proving the oxidative stress we established the level of malondialdehyde using a method with thiobarbituric acid TBA (Kei Satoh 1978) and the level of ceruloplasmin with the Ravin method .Also AST, ALT,CRP, iron, uric acid, urea were assessed.High level of homocysteine in both groups of study in comparison with the control group was found. The concentration of MDA was significantly higher in pregnancies complicated with risk of abortion and preterm birth compared to the control group (p=0.040, p=0.031). Considerable differences of ceruloplasmin concentration between group 1 and group 2 (p=0.045), and between group 2 and control group (p=0.034), was noticed but not any important differences between group 1 and control group (p=0.683). In women with risk of abortion or with preterm birth an oxidative stress and a hyperhomocysteinemia are present. PMID:22574089

  1. Pre-conditioning induces the precocious differentiation of neonatal astrocytes to enhance their neuroprotective properties

    Directory of Open Access Journals (Sweden)

    Sandra J Hewett

    2011-07-01

    Full Text Available Hypoxic preconditioning reprogrammes the brain's response to subsequent H/I (hypoxia–ischaemia injury by enhancing neuroprotective mechanisms. Given that astrocytes normally support neuronal survival and function, the purpose of the present study was to test the hypothesis that a hypoxic preconditioning stimulus would activate an adaptive astrocytic response. We analysed several functional parameters 24 h after exposing rat pups to 3 h of systemic hypoxia (8% O2. Hypoxia increased neocortical astrocyte maturation as evidenced by the loss of GFAP (glial fibrillary acidic protein-positive cells with radial morphologies and the acquisition of multipolar GFAP-positive cells. Interestingly, many of these astrocytes had nuclear S100B. Accompanying their differentiation, there was increased expression of GFAP, GS (glutamine synthetase, EAAT-1 (excitatory amino acid transporter-1; also known as GLAST, MCT-1 (monocarboxylate transporter-1 and ceruloplasmin. A subsequent H/I insult did not result in any further astrocyte activation. Some responses were cell autonomous, as levels of GS and MCT-1 increased subsequent to hypoxia in cultured forebrain astrocytes. In contrast, the expression of GFAP, GLAST and ceruloplasmin remained unaltered. Additional experiments utilized astrocytes exposed to exogenous dbcAMP (dibutyryl-cAMP, which mimicked several aspects of the preconditioning response, to determine whether activated astrocytes could protect neurons from subsequent excitotoxic injury. dbcAMP treatment increased GS and glutamate transporter expression and function, and as hypothesized, protected neurons from glutamate excitotoxicity. Taken altogether, these results indicate that a preconditioning stimulus causes the precocious differentiation of astrocytes and increases the acquisition of multiple astrocytic functions that will contribute to the neuroprotection conferred by a sublethal preconditioning stress.

  2. A selected reaction monitoring-based analysis of acute phase proteins in interstitial fluids from experimental equine wounds healing by secondary intention.

    Science.gov (United States)

    Bundgaard, Louise; Bendixen, Emøke; Sørensen, Mette Aa; Harman, Victoria M; Beynon, Robert J; Petersen, Lars J; Jacobsen, Stine

    2016-05-01

    In horses, pathological healing with formation of exuberant granulation tissue (EGT) is a particular problem in limb wounds, whereas body wounds tend to heal without complications. Chronic inflammation has been proposed to be central to the pathogenesis of EGT. This study aimed to investigate levels of inflammatory acute phase proteins (APPs) in interstitial fluid from wounds in horses. A novel approach for absolute quantification of proteins, selected reaction monitoring (SRM)-based mass spectrometry in combination with a quantification concatamer (QconCAT), was used for the quantification of five established equine APPs (fibrinogen, serum amyloid A, ceruloplasmin, haptoglobin, and plasminogen) and three proposed equine APPs (prothrombin, α-2-macroglobulin, and α-1-antitrypsin). Wound interstitial fluid was recovered by large pore microdialysis from experimental body and limb wounds from five horses at days 1, 2, 7, and 14 after wounding and healing without (body) and with (limb) the formation of EGT. The QconCAT included proteotypic peptides representing each of the protein targets and was used to direct the design of a gene, which was expressed in Escherichia coli in a media supplemented with stable isotopes for metabolically labeling of standard peptides. Co-analysis of wound interstitial fluid samples with the stable isotope-labeled QconCAT tryptic peptides in known amounts enabled quantification of the APPs in absolute terms. The concentrations of fibrinogen, haptoglobin, ceruloplasmin, prothrombin, and α-1-antitrypsin in dialysate from limb wounds were significantly higher than in dialysate from body wounds. This is the first report of simultaneous analysis of a panel of APPs using the QconCAT-SRM technology. The microdialysis technique in combination with the QconCAT-SRM-based approach proved useful for quantification of the investigated proteins in the wound interstitial fluid, and the results indicated that there is a state of sustained inflammation in

  3. Systemic Inflammation in Cardiovascular and Periodontal Disease: Comparative Study

    Science.gov (United States)

    Glurich, Ingrid; Grossi, Sara; Albini, Boris; Ho, Alex; Shah, Rashesh; Zeid, Mohamed; Baumann, Heinz; Genco, Robert J.; De Nardin, Ernesto

    2002-01-01

    Epidemiological studies have implicated periodontal disease (PD) as a risk factor for the development of cardiovascular disease (CVD). These studies addressed the premise that local infection may perturb the levels of systemic inflammatory mediators, thereby promoting mechanisms of atherosclerosis. Levels of inflammatory mediators in the sera of subjects with only PD, only CVD, both diseases, or neither condition were compared. Subjects were assessed for levels of C-reactive protein (CRP), serum amyloid A (SAA), ceruloplasmin, α1-acid-glycoprotein (AAG), α1-antichymotrypsin (ACT), and the soluble cellular adhesion molecules sICAM-1 and sVCAM by enzyme-linked immunoabsorbent and/or radial immunodiffusion assays. CRP levels in subjects with either condition alone were elevated twofold above subjects with neither disease, whereas a threefold increase was noted in subjects with both diseases (P = 0.0389). Statistically significant increases in SAA and ACT were noted in subjects with both conditions compared to those with one or neither condition (P = 0.0162 and 0.0408, respectively). Ceruloplasmin levels were increased in subjects with only CVD (P = 0.0001). Increases in sVCAM levels were noted in all subjects with CVD (P = 0.0054). No differences in sICAM levels were noted among subject groups. A trend toward higher levels of AAG was noted in subjects with both conditions and for ACT in subjects with only PD. Immunohistochemical examination of endarterectomy specimens of carotid arteries from subjects with atherosclerosis documented SAA and CRP deposition in association with atheromatous lesions. The data support the hypothesis that localized persistent infection may influence systemic levels of inflammatory mediators. Changes in inflammatory mediator levels potentially impact inflammation-associated atherosclerotic processes. PMID:11874889

  4. Western United States field trials and radioisotopic investigations on the molybdenum-copper interactions in livestock

    International Nuclear Information System (INIS)

    A review of western United States field trials on the molybdenum-copper interaction in cattle is presented. Historically, investigations in the western United States of America have focused on techniques to improve livestock production by copper supplementation. Initially, to evaluate copper-responsive situations, the molybdenum content of plant tissues was explored, and techniques were developed to reduce molybdenum levels in forage. Later, molybdenum levels were evaluated in combination with dietary copper and sulphate levels, and their relation to the occurrence of molybdenosis in livestock. Efforts are now directed at determining efficient methods of direct copper supplementation of livestock. Present techniques used in the western United States for copper supplementation include addition of copper sulphate to the diet or injection of copper glycinate. Current concepts of the Mo-Cu-SO4 interactions are discussed. Based on a review of radioisotopic investigations in ruminants, a mechanism for molybdenum-antagonized copper utilization by the liver includes the hypothesis that molybdenum does not interfere with copper transport to the liver, but interferes with the synthesis of the plasma enzyme, ceruloplasmin (Cp). The resultant apoceruloplasmin-like substances, with fewer copper atoms per molecule and less enzyme activity, are catabolized more rapidly than holoceruloplasmin. Such compounds have been reported in the literature. The higher levels of 64Cu in both the plasma and faeces of Mo-treated animals are possibly the result of a greater utilization of 64Cu in the synthesis of ceruloplasmin molecules with some apoproteins, which are more rapidly catabolized and subsequently excreted via bile to faeces. In this case, faecal loss of absorbed copper would be a major route of copper loss in molybdenum-induced copper deficiencies. (author)

  5. A synthetic peptide with the putative iron binding motif of amyloid precursor protein (APP does not catalytically oxidize iron.

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    Kourosh Honarmand Ebrahimi

    Full Text Available The β-amyloid precursor protein (APP, which is a key player in Alzheimer's disease, was recently reported to possess an Fe(II binding site within its E2 domain which exhibits ferroxidase activity [Duce et al. 2010, Cell 142: 857]. The putative ligands of this site were compared to those in the ferroxidase site of ferritin. The activity was indirectly measured using transferrin, which scavenges the Fe(III product of the reaction. A 22-residue synthetic peptide, named FD1, with the putative ferroxidase site of APP, and the E2 domain of APP were each reported to exhibit 40% of the ferroxidase activity of APP and of ceruloplasmin. It was also claimed that the ferroxidase activity of APP is inhibited by Zn(II just as in ferritin. We measured the ferroxidase activity indirectly (i by the incorporation of the Fe(III product of the ferroxidase reaction into transferrin and directly (ii by monitoring consumption of the substrate molecular oxygen. The results with the FD1 peptide were compared to the established ferroxidase activities of human H-chain ferritin and of ceruloplasmin. For FD1 we observed no activity above the background of non-enzymatic Fe(II oxidation by molecular oxygen. Zn(II binds to transferrin and diminishes its Fe(III incorporation capacity and rate but it does not specifically bind to a putative ferroxidase site of FD1. Based on these results, and on comparison of the putative ligands of the ferroxidase site of APP with those of ferritin, we conclude that the previously reported results for ferroxidase activity of FD1 and - by implication - of APP should be re-evaluated.

  6. CHANGING METABOLIC FUNCTIONS IN EXPERIMENTAL ANIMALS AFTER INTRODUCTION OF THE XENOBIOTIC, IMMUNOTROPIC DRUG AND PROBIOTIC

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    Zvyagintseva O.V.

    2015-05-01

    Full Text Available The aim of the study was to evaluate in vivo changes in metabolic and barrier function of the resistance factors (activity of enzymes of neutrophils, the efficiency of phagocytosis, some biochemical parameters (concentration of ceruloplasmin and haptoglobin and proliferate activity in vitro cells after introduction of copper sulfate, probiotics and immunostimulant "Fungidol" the experimental animals. Material and methods. The in vivo experiments were performed on 6-month-old male rats of Wistar line. Identified the following groups: group 1 - control animals, which were intraperitoneally injected with saline (n = 5; group 2 - animals that were administered saline per os and 48 hours a solution of copper sulphate intraperitoneally (n = 5; group 3 - animals, which were injected with immunotropic drug "Fungidol" per os and 48 hours a solution of copper sulphate intraperitoneally (n = 5; group 4 animals, which were injected with a solution of probiotics per os and 48 hours a solution of copper sulphate intraperitoneally (n = 5. As a probiotic used capsules firm Yogurt that contains active Lactobacillus acidophilus, Lactobacillus rhamnosus, Streptococcus thermophillus, Lactobacillus bulgaricus. The concentration of haptoglobin and ceruloplasmin were determined spectrophotometrically. Oxygen-dependent metabolism of neutrophils was investigated by microscopy according to their ability to absorb nitroblue tetrazolium (NBT-test and restore it to deformazione in the form of granules blue color under the influence of superoxide anion, which is formed in the NADP-oxidase reaction, initiating the process of stimulation of phagocytosis (NBT-test. To determine the barrier function of phagocytic cells by light microscopy to evaluate the activity of phagocytosis of neutrophilic granulocytes with subsequent determination of phagocytic index, phagocytic number and the index of completeness of phagocytosis. As a microbial agent used is a suspension culture of

  7. Wilson's disease in children: clinical and diagnostic features

    International Nuclear Information System (INIS)

    Objective: To study the clinical and diagnostic laboratory features of Wilsons disease in children and adolescents. Design: A prospective cohort study. Place and Duration of study: The study included patients diagnosed as Wilson s disease at the Department of Pediatrics Allied Hospital, Punjab medical College, Faisalabad from May 1997 to June 2001. Patients and methods: Patients presenting with liver or suggestive neurological disease were investigated. Others were diagnosed as a result of family screening. Diagnosis of neurologic disease was made if two of the following were present: Typical neurological findings, Kayser Fleischer corneal rings and low serum ceruloplasmin (100 mu gm) free serum copper (>10 mu gm/dl). In other forms and for family screening, 24 hours. Urinary copper (> 100 mu gm), free serum copper (>10 mu gm/dl), and wherever possible liver biopsy for histopathology and cytochemical staining by rubeanic acid was also done. Results: Twenty-seven patients with a mean age of 10.2 years were diagnosed as suffering from Wilson disease. Mean age for hepatic and neurological disease was 9 years and 11.5 years respectively. Youngest patient (neurologic) was 6 years old. 48% cases presented with neurological, 41% with hepatic and 4% with skeletal manifestations while 7 % were asymptomatic. Mean duration of symptoms before diagnosis was 6.1 months. Dysarthria (84.6%), tremors (69.2%), rigidity and poor school performance and hand writing (61.5%), dysphagia (46.1%) and dystonia (38.5%), were the most common neurologic findings. Chronic liver disease was seen in 73 % while acute forms were seen in 27 % cases. Two cases presented with fulminant hepatic failure. Consanguineous marriage of the parents was found in 70 % and family history of disease was present in 65 % cases. K-F (Kayser Fleischer) rings and low serum ceruloplasmin(<20 mg/dl) was found in 85% of all patients. In non neurologic types other tests of copper metabolism were done. Elevated urinary

  8. Relationship of concentrations of cortisol in hair with health, biomarkers in blood, and reproductive status in dairy cows.

    Science.gov (United States)

    Burnett, Tracy A; Madureira, Augusto M L; Silper, Bruna F; Tahmasbi, Abdolmansour; Nadalin, Audrey; Veira, Douglas M; Cerri, Ronaldo L A

    2015-07-01

    Hair cortisol has been used to measure chronic stress in dairy cows as it offers the advantage of being noninvasive, fast, and able to indicate levels of cortisol over long periods. The aim of this study was to determine the associations between hair cortisol with clinical disorders, reproductive status, and the development of subclinical endometritis in dairy cows. Furthermore, we aimed to determine the association between hair cortisol concentrations and blood markers associated with metabolic status and acute inflammation. In experiment 1, cows (n=64) were hair sampled every 3wk from the tail switch beginning at calving (d 0) until d 126 for cortisol analysis; blood samples were collected every 3wk from d 0 until 42 for β-hydroxybutyrate and glucose analysis. In experiment 2, cows (n=54) were chosen retrospectively by diagnosis of subclinical endometritis (END), subclinical endometritis and at least 1 clinical disease (END+CLIN), or as healthy (control) using a cytobrush and ultrasonography at 30±3d in milk. At the same time, animals were hair sampled for cortisol analysis and blood sampled for haptoglobin and ceruloplasmin analysis. Health records were recorded throughout both experimental periods. Animals with clinical disease presented higher cortisol concentrations than clinically healthy animals in experiment 1 [geometric mean (95% confidence interval); 8.8 (7.8, 9.9) vs. 10.7 (9.6, 12.0) pg/mg]; however, animals diagnosed with subclinical endometritis in experiment 2 did not differ in hair cortisol concentrations [11.7 (9.8, 14.0), 12.2 (9.3, 15.9), 10.5 (8.1, 13.6) pg/mg for control, END, and END+CLIN, respectively]. In experiment 1, an effect of sample day was noted, where d 21 had higher cortisol concentrations than d 42, 84, and 126, but not from d 0 for both parities. Within both experiments, a parity effect was present where multiparous animals consistently had higher cortisol concentrations than primiparous animals. Multiparous cows that became

  9. Effects of grain, fructose, and histidine feeding on endotoxin and oxidative stress measures in dairy heifers.

    Science.gov (United States)

    Golder, H M; Lean, I J; Rabiee, A R; King, R; Celi, P

    2013-01-01

    Ruminal endotoxin and plasma oxidative stress biomarker concentrations were studied in dairy heifers challenged with grain, fructose, and histidine in a partial factorial study. Holstein-Friesian heifers [n=30; average body weight (BW) of 359.3±47.3 kg] were randomly allocated to 5 treatment groups: (1) control (no grain); (2) grain [crushed triticale at 1.2% of BW dry matter intake (DMI)]; (3) grain (0.8% of BW DMI) + fructose (0.4% of BW DMI); (4) grain (1.2% of BW DMI) + histidine (6g/head); and (5) grain (0.8% of BW DMI) + fructose (0.4% of BW DMI) + histidine (6 g/head). Rumen samples were collected by stomach tube 5, 65, 115, 165, and 215 min after diet consumption and blood samples at 5 and 215 min after consumption. Rumen fluid was analyzed for endotoxin concentrations. Plasma was analyzed for concentrations of the following oxidative stress biomarkers: reactive oxygen metabolites (dROM), biological antioxidant potential (BAP), advanced oxidation protein products, and ceruloplasmin, and activity of glutathione peroxidase. Dietary treatment had no effect on concentrations of endotoxin or oxidative stress biomarkers. We observed no interactions of treatment by time. Ruminal concentrations of endotoxin decreased during the sampling period from 1.12×10(5) ± 0.06 to 0.92×10(5) endotoxin units/mL ± 0.05 (5 and 215 min after diet consumption, respectively). Concentrations of dROM and the oxidative stress index (dROM/BAP × 100) increased over the sampling period, from 108.7 to 123.5 Carratelli units (Carr U), and from 4.1 to 4.8, respectively. Ceruloplasmin concentrations markedly declined 5 min after the consumption of diets, from 190 to 90 mg/L over the 215-min sampling period. Overall, a single feeding challenge for dairy cattle with grain, fructose, and histidine, and combinations thereof, may not be sufficient to induce marked changes in endotoxin or oxidative stress biomarker concentrations. PMID:24119801

  10. Leucograma e teores plasmáticos de proteínas de fase aguda de eqüinos portadores de abdômen agudo e submetidos à laparotomia Leukogram and plasma acute phase protein concentrations in horses with acute abdomen submitted to treatment by laparotomy

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    J.J. Fagliari

    2008-04-01

    Full Text Available Foram examinados 20 eqüinos adultos portadores de abdômen agudo e submetidos à laparotomia. Dez recuperaram-se sem intercorrência pós-operatória (G1 e 10 foram a óbito sete a 10 dias após a cirurgia, com sinais de choque séptico (G2. Avaliaram-se temperatura retal, freqüências cardíaca e respiratória, tempo de preenchimento capilar e teores plasmáticos das proteínas de fase aguda - fibrinogênio, ceruloplasmina, proteína C-reativa, antitripsina, haptoglobina e glicoproteína ácida -, antes e até sete dias após a laparotomia. As leucometrias às 72h e no sétimo dia pós-operatório dos eqüinos que foram a óbito foram, respectivamente, 34,6% e 57,1%, mais altas que a dos animais curados. Os maiores valores de proteína de fase aguda ocorreram no sétimo dia após a cirurgia; os percentuais de elevação de fibrinogênio, antitripsina, glicoproteina ácida, proteína C-reativa, ceruloplasmina e haptoglobina de eqüinos do G2 em relação ao G1 foram 46,8%, 67,9%, 91,9%, 112,2%, 126,9% e 186,2%, respectivamente.Twenty adult horses with acute abdomen were examined and submitted to treatment by laparotomy; ten had no postoperative complication (group 1, and ten showed septic shock symptom and died from seven to ten days after surgery (group 2. Body temperature, heart and respiratory rates, filling capillary time, and plasma acute phase protein concentrations - fibrinogen, ceruloplasmin, C-reactive protein, antitrypsin, haptoglobin, and acid glycoprotein - were evaluated before laparotomy and until seven days after surgery. White blood cell counts at 72h and seven days after surgery in group 2 animals were, respectively, 34.6% and 57.1%, and were higher than those measured in group 1 horses. The highest values of acute phase proteins occurred on the seventh day after surgery. The increase percentages of fibrinogen, antitrypsin, acid glycoprotein, C-reactive protein, ceruloplasmin, and haptoglobin plasma concentrations in group 2

  11. Associação da concentração plasmática de cobre com metaloproteínas cobre-dependentes em atletas de elite Association between copper plasma concentration and copper-dependent metaloproteins in elite athletes

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    Josely Correa Koury

    2007-08-01

    Full Text Available O cobre é um elemento-traço essencial para a manutenção de vários processos biológicos, tais como metabolismo energético, homeostase de ferro e mecanismos de proteção antioxidante através da atividade da cobre-zinco superóxido dismutase (Cu-Zn SOD, da ceruloplasmina e da metalotioneína. No entanto, o cobre também participa de reações oxidativas que promovem a liberação de radicais livres, podendo prejudicar a integridade e a funcionalidade celular. A atividade física afeta a homeostase do cobre e promove maior utilização de oxigênio, favorecendo a instalação do estresse oxidativo quando mecanismos naturais de proteção antioxidante, incluindo os dependentes de cobre, não atuam adequadamente. Não há relatos na literatura sobre a associação de diferentes níveis de concentração plasmática de cobre com indicadores antioxidantes cobre-dependentes em atletas de elite. O presente estudo objetivou verificar a associação entre diferentes níveis plasmáticos de cobre e metaloproteínas cobre-dependentes, com atividade antioxidante, em atletas de elite. Os indicadores bioquímicos (metalotioneína e Cu-Zn SOD eritrocitárias, ceruloplasmina e cobre plasmáticos foram avaliados em 50 atletas, homens e adultos, utilizando metodologias já consolidadas. Os resultados mostraram que 32% dos atletas apresentaram níveis de cobre plasmático inferiores a 11µmol/L, 38% entre 11-13µmol/L e 30% > 13µmol/L. As associações encontradas entre cobre plasmático e ceruloplasmina (r = 0,31; p = 0,04 e Cu-Zn SOD (r = 0,32, p = 0,02; metalotioneína eritrocitária e ceruloplasmina (r = 0,40, p = 0,006 e Cu-Zn SOD (0,73, p = 0,001 e entre Cu-Zn SOD e ceruloplasmina (r = 0,37, p Copper is a trace element essential in several biological processes, some of them important for physical activity, such as energy metabolism, iron homeostasis and antioxidant protection through the plasma ceruloplasmin, erythrocyte Cu-Zn superoxide dismutase (Cu

  12. Serial CT scans and Menkes' kinky hair disease

    International Nuclear Information System (INIS)

    Menkes' kinky hair disease is a sex-linked recessive disorder of copper metabolism, characterized by progressive psychomotor deterioration, seizures, and peculiar hair structure. We examined serial CT scans of patients with this disease. A 2,210-g male infant was delivered after an uneventful gestation of 36 weeks. His one-minute Apgar score was 9. His uncle had died at 1 year of age. His first cousin was also diagnosed as having Menkes' kinky hair disease when our patient was 2 years old. Shortly after birth he had mild respiratory distress. At 5 days of age, he developed setting-sun signs. The first CT scan, at 10 days of age, revealed mild posterior fossa hemorrhages. At 3 months of age, myoclonic seizures began, and the CT scan revealed subdural effusion and mild brain atrophy. The seizures were controllable by using phenobarbital, valproic acid, and nitrazepam. He did not follow light or a fixate, but the fundi were normal. He was diffusely hypotonic. At 9 months of age, the seizures became uncontrollable. The CT scans at 15 and 31 months of age showed subdural hemorrhage and/or brain atrophy. At 15 months of age, the serum copper level was 42 μ g/dl, while the serum ceruloplasmin level was 3.2 mg/dl. He exhibited severe developmental failure. At 4 years of age, he died. (author)

  13. Serum protein concentrations in calves with experimentally induced pneumonic pasteurellosis

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    Fagliari J.J.

    2003-01-01

    Full Text Available Ten healthy 2 to 4-week-old Holstein calves were randomly allotted into control and infected groups. Control calves (n=5 were inoculated intrabronchially with 5ml of Dulbecco's phosphate-buffered saline solution (DPBSS. Infected calves (n=5 were inoculated intrabronchially with 5x10(9 log-phase Mannheimia haemolytica organisms suspended in 5ml of DPBSS. Blood samples were obtained 15 minutes before and one, two, four and six hours after inoculation. Serum protein concentrations were determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Serum concentrations of proteins with molecular weights of 125,000 D (ceruloplasmin, 60,000 D (a 1-antitrypsin, 45,000 D (haptoglobin, and 40,000 D (acid glycoprotein were significantly increased in calves with pneumonic pasteurellosis, compared with concentrations in control calves. Results indicate that acute phase proteins increase more rapidly after the onset of inflammation than previously thought. Measurement of serum protein concentrations may be useful in monitoring the progression of the induced pneumonic pasteurellosis in calves.

  14. Oral Zinc Supplementation Decreases the Serum Iron Concentration in Healthy Schoolchildren: A Pilot Study

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    Naira Josele Neves de Brito

    2014-09-01

    Full Text Available The recognized antagonistic actions between zinc and iron prompted us to study this subject in children. A convenience sample was used. Thirty healthy children between 8 and 9 years of age were studied with the aim of establishing the effect of a 3-mo oral zinc supplementation on iron status. Fifteen individuals were given a placebo (control group, and 15 were given 10 mg Zn/day (experimental group. Blood samples were collected at 0, 60, 120, 180 and 210 min after a 12-h overnight fast, before and after placebo or zinc supplementation. This supplementation was associated with significant improvements in energy, protein, fat, carbohydrate, fiber, calcium, iron, and zinc intake in accordance with the recommendations for age and sex. The basal serum zinc concentration significantly increased after oral zinc supplementation (p < 0.001. However, basal serum iron concentrations and area under the iron curves significantly decreased in the experimental group (p < 0.0001 and remained at the same level throughout the 210-min study. The values obtained for hemoglobin, mean corpuscular volume, ferritin, transferrin, transferrin saturation, ceruloplasmin and total protein were within normal reference ranges. In conclusion, the decrease in serum iron was likely due to the effects of chronic zinc administration, and the decrease in serum iron was not sufficient to cause anemia.

  15. Copper and Copper Proteins in Parkinson’s Disease

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    Sergio Montes

    2014-01-01

    Full Text Available Copper is a transition metal that has been linked to pathological and beneficial effects in neurodegenerative diseases. In Parkinson’s disease, free copper is related to increased oxidative stress, alpha-synuclein oligomerization, and Lewy body formation. Decreased copper along with increased iron has been found in substantia nigra and caudate nucleus of Parkinson’s disease patients. Copper influences iron content in the brain through ferroxidase ceruloplasmin activity; therefore decreased protein-bound copper in brain may enhance iron accumulation and the associated oxidative stress. The function of other copper-binding proteins such as Cu/Zn-SOD and metallothioneins is also beneficial to prevent neurodegeneration. Copper may regulate neurotransmission since it is released after neuronal stimulus and the metal is able to modulate the function of NMDA and GABA A receptors. Some of the proteins involved in copper transport are the transporters CTR1, ATP7A, and ATP7B and the chaperone ATOX1. There is limited information about the role of those biomolecules in the pathophysiology of Parkinson’s disease; for instance, it is known that CTR1 is decreased in substantia nigra pars compacta in Parkinson’s disease and that a mutation in ATP7B could be associated with Parkinson’s disease. Regarding copper-related therapies, copper supplementation can represent a plausible alternative, while copper chelation may even aggravate the pathology.

  16. GST null genotype and antioxidants: Risk indicators for oral pre-cancer and cancer

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    Bathi Renuka

    2009-01-01

    Full Text Available Objectives : This study was undertaken to detect the gene polymorphism of detoxification enzymes and estimate the antioxidant enzyme status in patients with oral cancer, oral leukoplakia and oral submucous fibrosis (OSF. Materials and Methods : The GSTM1 and GSTT1 gene polymorphism was evaluated using polymerase chain reaction; the antioxidant enzyme was estimated using biochemical methods. Statistical analyses were performed using student t-test and odds-ratio to estimate relative risk (RR. Results : The RR at 95% confidence interval (CI for GSTM1 and GSTT1 was statistically significant for all groups. The mean values of glutathione were significantly raised in all groups. The mean values of ceruloplasmin and malonaldehyde was statistically significant among cancer and OSF patients but was insignificant in smokers and cases with leukoplakia. Conclusion : Several genes perform the same function which implies the need to test for several genetic polymorphisms to identify individuals at high risk. The level of antioxidant enzymes correlate with the degree of oxidative damage. The need for further studies is emphasised.

  17. Electrophoretic variants of blood proteins in japanese, 5

    International Nuclear Information System (INIS)

    The plasma ceruloplasmin (CP) of 22,367 children of atomic bomb survivors in Hiroshima and Nagasaki was examined for variants by electrophoresis. The sample was composed of 14,964 unrelated children and 7,403 siblings of the unrelated persons. A total of seven types of electrophoretic variants were detected; four migrating anodally and three cathodally to the normal B band. We have reported two of these variants, CP A sub(NG1) and CP C sub(NG1), previously but the other five, CP A sub(NG2), CP A sub(HR1), CP A sub(HR2), CP C sub(HR1), and CP C sub(HR2), are newly identified. The allelic frequency of CP*CNG1 was 0.00916, so that the variant is considered to be a polymorphic allele. Homozygosity for the CP*CNG1 allele was detected in five individuals. This is the first report of a homozygous phenotype for a CP variant in a Japanese population. Family study of the new five variants all demonstrated patterns of codominant inheritance. (author)

  18. Urinary proteomic shotgun approach for identification of potential acute rejection biomarkers in renal transplant recipients

    Science.gov (United States)

    2012-01-01

    Background Acute rejection (AR) episodes in renal transplant recipients are suspected when plasma creatinine is elevated and other potential causes out ruled. Graft biopsies are however needed for definite diagnosis. Non-invasive AR-biomarkers is an unmet clinical need. The urinary proteome is an interesting source in the search for such a biomarker in this population. Methods In this proof of principle study, serial urine samples in the early post transplant phase from 6 patients with biopsy verified acute rejections and 6 age-matched controls without clinical signs of rejection were analyzed by shotgun proteomics. Results Eleven proteins fulfilled predefined criteria for regulation in association with AR. They presented detectable regulation already several days before clinical suspicion of AR (increased plasma creatinine). The regulated proteins could be grouped by their biological function; proteins related to growth and proteins related to immune response. Growth-related proteins (IGFBP7, Vasorin, EGF and Galectin-3-binding protein) were significantly up-regulated in association with AR (P = 0.03) while proteins related to immune response (MASP2, C3, CD59, Ceruloplasmin, PiGR and CD74) tended to be up-regulated ( P = 0.13). Conclusion The use of shotgun proteomics provides a robust and sensitive method for identification of potentially predictive urinary biomarkers of AR. Further validation of the current findings is needed to establish their potential clinical role with regards to clinical AR diagnosis. Trial registration ClinicalTrials.gov number NCT00139009 PMID:23369437

  19. Development of technology for biological dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Kim, In Gyu; Kim, Kug Chan; Lee, Kang Sik; Kim, Jin Kyu; Chun, Ki Jung; Shim, Hye Won; Park, Seon Young

    1997-07-01

    Adult male rats were treated a single, whole body exposure to a dose of 0.1, 0.5, 1, 2, 3, 5, 7 and 9 Gy. The animal were sacrificed 6, 24, 48, 72, 96 and 212 hours following exposure. Enzyme activity changes such as alkaline phosphatase, GOT, creating kinase, MDH and LDH in rat serum as biochemical indicators useful for evaluating radiation exposure were measured. An competitive enzyme linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum after gamma-irradiation. Rat alpha-2 macroglobulin, alpha-1 acid glycoprotein, haptoglobin, ceruloplasmin, C-reactive protein and alpha-1 antitrypsin were purified from the plasma of turpentine treated rats. In vitro somatic mutation induced by gamma-irradiation and pentachlorophenol (PCP) which is representative of chemical pollutant was measured at the hypoxanthine-guanine phosphorybosyl transferase (HPRT) locus in human T-lymphocytes by a cell cloning assay. Mutant cells were selected by their ability to form a clone in the presence of purine analogue 6-thioguanine. Reverse transcriptase/polymerase chain reaction technique was needed for the mutation spectrum to discriminate combined exposure to radiation and chemicals. (author). 98 refs., 7 tabs., 47 figs.

  20. Acute phase proteins in serum and cerebrospinal fluid in the course of bacterial meningitis.

    Science.gov (United States)

    Paradowski, M; Lobos, M; Kuydowicz, J; Krakowiak, M; Kubasiewicz-Ujma, B

    1995-08-01

    We carried out estimations of the following acute phase proteins: C-reactive protein (CRP), alpha-1-antitrypsin (AAT), alpha-1-acid glycoprotein (AAG), alpha-2-ceruloplasmin (CER), and alpha-2-haptoglobin (HPT) in serum and in cerebrospinal fluid (CSF) in patients with bacterial meningitis (BM, n = 30) and viral meningitis (VM, n = 30). We have shown that determinations of concentrations of AAG and CRP in serum and CER in CSF are useful in differentiation between BM and VM. The diagnostic power of these three tests (the areas under their ROC curves equal 0.942, 0.929, and 0.931, respectively) is bigger, though statistically not significantly, than that of traditional parameters of BM in CSF, i.e., total protein concentration and white blood cell count. Determination of AAG, CRP, and AAT in serum is a valuable monitoring marker in the course of BM treatment. Convenience of serum sampling constitutes an advantage over traditional BM parameters in CSF. PMID:8521602

  1. Importance and significance of liver echotomography in Wilson disease childhood

    International Nuclear Information System (INIS)

    We studed the hepatic echography patterns of young patients suffering from Wilson's Disease and compared these with results obtained from laboratory tests and parenchymal biopsy. Eight children, aged between 5 and 12 years (4 males and 4 females), were examined. The symptomatological pattern showed hepatomegaly and liver dysfunction with undetectable or very low serum ceruloplasmine levels and typical aspects of organic copper metabolism. In 50% of cases echography revealed a variable hyperreflection of the whole parenchyma, in connection with the presence of steatosis and aspects of intralobular inflammation. Fine and linear echoes were present, in 50% of the cases, with varying degrees of brightness, which was associated with the presence of fibrotic component or copper granules. Further echographic modifications were less frequent. Echography in close relatives of our patients was observed hepatomegaly and alteration of hepatic reflection associated with fibrotic component. In the evaluation of liver involvement in Wilson's Disease, these findings show that echotomography, when compared to hepatic biopsy, is less efficient in detecting different types of hepatic lesion but on a clinical basis it offers the possibility of evaluating their presence and gravity during the evolutive phase of the illness. This examination, unlike other methods, is completely non invasive; it may easily be repeated and may, in the future, come to be used as more precise diagnostic complement. 30 refs

  2. P wave dispersion is prolonged in patients with Wilson's disease

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    AIM: To investigate the P wave dispersion as a noninvasive marker of intra-atrial conduction disturbances in patients with Wilson's disease. METHODS: We compared Wilson's disease patients (n=18) with age matched healthy subjects (n=15) as controls. The diagnosis was based on clinical symptoms, laboratory tests (ceruloplasmin, urinary and hepatic copper concentrations). P wave dispersion, a measurement of the heterogeneity of atrial depolarization, was measured as the difference between the duration of the longest and the shortest P-waves in 12 lead electrocardiography. RESULTS: All the patients were asymptomatic on cardiological examination and have sinusal rhythm in electrocardiography. Left ventricular and left atrial diameters, left ventricular ejection fraction and left ventricular mass index were similar in both groups. The Wilson's disease patients had a significantly higher P wave dispersion compared with the controls (44.7+5.8vs 25.7±2.5,P<0.01=. CONCLUSION: There was an increase in P wave dispersion in cardiologically asymptomatic Wilson's disease patients which probably represents an early stage of cardiac involvement.

  3. Aceruloplasminemia With Psychomotor Excitement and Neurological Sign Was Improved by Minocycline (Case Report).

    Science.gov (United States)

    Hayashida, Maiko; Hashioka, Sadayuki; Miki, Hiroyuki; Nagahama, Michiharu; Wake, Rei; Miyaoka, Tsuyoshi; Horiguchi, Jun

    2016-05-01

    Aceruloplasminemia is an autosomal recessive disorder of iron metabolism caused by mutations in the ceruloplasmin gene. Its prevalence is 1 in 2,000,000 people in Japan. This is a disorder of neurodegeneration with iron accumulation in the brain revealed by MRI. The iron overload induces oxidative stress and generation of reactive oxygen species, which triggers a cascade of pathological events that lead to neuronal death. Intravenous administration of an iron chelator, deferoxamine has been proposed as a method of inhibiting the accumulation of iron.The patient was a 46-year-old Japanese woman. She was diagnosed at the age of 33 years. Deferoxamine was administrated for 6 months but was discontinued due to adverse effects. On admission at the age of 46, psychomotor excitement was acute in onset. The extrapyramidal symptoms reflected iron deposition in the basal ganglia and substantia nigra in the midbrain. Ataxia and a wide-based gate reflected iron deposition in the dentate nuclei of the cerebellum. An antibiotic, minocycline at 150 mg/day successfully ameliorated the clinical symptoms.Minocycline, a second generation tetracycline, has a direct radical scavenging property due to its chemical structure. It has been reported that minocycline is similar to deferoxamine in its ability to chelate iron. Minocycline is also involved in preventing the upregulation of proinflammatory cytokines. The iron-chelating, antioxidant, and anti-inflammatory effects of minocycline were involved in this case. PMID:27175663

  4. Minocycline Attenuates Iron-Induced Brain Injury.

    Science.gov (United States)

    Zhao, Fan; Xi, Guohua; Liu, Wenqaun; Keep, Richard F; Hua, Ya

    2016-01-01

    Iron plays an important role in brain injury after intracerebral hemorrhage (ICH). Our previous study found minocycline reduces iron overload after ICH. The present study examined the effects of minocycline on the subacute brain injury induced by iron. Rats had an intracaudate injection of 50 μl of saline, iron, or iron + minocycline. All the animals were euthanized at day 3. Rat brains were used for immunohistochemistry (n = 5-6 per each group) and Western blotting assay (n = 4). Brain swelling, blood-brain barrier (BBB) disruption, and iron-handling proteins were measured. We found that intracerebral injection of iron resulted in brain swelling, BBB disruption, and brain iron-handling protein upregulation (p < 0.05). The co-injection of minocycline with iron significantly reduced iron-induced brain swelling (n = 5, p < 0.01). Albumin, a marker of BBB disruption, was measured by Western blot analysis. Minocycline significantly decreased albumin protein levels in the ipsilateral basal ganglia (p < 0.01). Iron-handling protein levels in the brain, including ceruloplasmin and transferrin, were reduced in the minocycline co-injected animals. In conclusion, the present study suggests that minocycline attenuates brain swelling and BBB disruption via an iron-chelation mechanism. PMID:26463975

  5. Effect of pumpkin-seed oil on the level of free radical scavengers induced during adjuvant-arthritis in rats.

    Science.gov (United States)

    Fahim, A T; Abd-el Fattah, A A; Agha, A M; Gad, M Z

    1995-01-01

    Pumpkin-seed oil (PSO), a natural supplement rich with antioxidant ingredients, was given to rats in which arthritis was induced using Freund's complete adjuvant. Its effect was compared with that of indomethacin, as a classical anti-inflammatory agent. Two experimental patterns were studied, an acute phase that was applied only with PSO and a chronic phase applied for both PSO and indomethacin. Compared to normal untreated rats, it was shown that the induction of arthritis caused a decrease in serum sulphhydryl groups, with an increase in serum ceruloplasmin in both phases. Blood glutathione was first elevated in the acute phase, then its level was reduced in the chronic phase. Serum N-acetyl-beta-D-glucosaminidase activity was elevated only at the acute phase, while plasma total proteins and albumin were reduced at the chronic phase. Liver glucose-6-phosphate dehydrogenase activity was markedly increased, while no changes were observed in the levels of liver lipid peroxides and glutathione. These changes in the studied parameters were attributed to the superoxides and free radicals during arthritic inflammation. Administration of PSO succeeded in modulating most of the altered parameters affected during arthritis, especially at the chronic phase. Also, a remarkable inhibition of paw oedema was observed. A similar pattern was obtained upon treatment with indomethacin except that indomethacin markedly elevated liver lipid peroxides levels. Concurrent administration of PSO with indomethacin caused no changes in the parameters studied compared to that induced by treatment with indomethacin alone. PMID:7784309

  6. 99Mo metabolism in sheep after the intravenous injection of [99Mo] thiomolybdates

    International Nuclear Information System (INIS)

    [99Mo]di-, tri-, and tetrathiomolybdate (5.4 to 62.5 mg of Mo) were given by intravenous injection to sheep maintained on a sulfur-supplemented (3 g of S/kg) diet. All the compounds were metabolized very rapidly over the first 15 min postinjection, but relatively slowly thereafter, with a t1/2 of about 30 hr for dithiomolybdate and 40 h for tri- and tetrathiomolybdate. The [99Mo] metabolites present in plasma were identified by Sephadex G-25 chromatography. The main fate of the compounds injected appeared to be stepwise transformation to molybdate and subsequent rapid urinary elimination. Over 90-100 hr more than 90% of the radioactivity was excreted in urine, compared to less than 5% in the feces. The trichloroacetic acid (TCA) solubility of plasma copper and the diamine oxidase activity of ceruloplasmin was depressed, particularly over the first 15 min postinjection and a more persistant TCA-insoluble Cu fraction was apparent

  7. Proteinograma sérico de bezerros recém-nascidos da raça Holandesa obtido por eletroforese em gel de poliacrilamida Serum protein concentration in newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis

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    J.J. Fagliari

    2006-06-01

    Full Text Available The serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15% of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW ranging from 28,000 D to 170,000D (proteinogram 1; 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1; and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3. The three groups presented similar IgG levels. The highest serum concentration of ceruloplasmin were verified in proteinogram 3, which had the lowest serum level of protein with MW 58,000D. It was verified a1-antitrypsin only in proteinogram 2, which had no proteins with MW of 42,000 D and 37,000D. The highest serum concentrations of IgA and protein with MW 58,000 D, and the lowest serum levels of transferrin, haptoglobin, and acid glycoprotein were verified in proteinogram 3. Measurement of serum protein concentrations by SDS-PAGE may be useful in monitoring the occurrence of hypogammaglobulinemia and the neonatal disease in calves.

  8. Postmortem Menkes diagnosis from carrier testing of female relatives.

    Science.gov (United States)

    Tønnesen, T; Silengo, M; Gerdes, A M; Hansen, J C; Reske-Nielsen, E; Franceschini, P; Horn, N

    1987-12-01

    A boy who died at 6 months of age was noted to have sparse, stubby and light hair, pili torti were observed microscopically, and his skin was dry and redundant. As a suspicion of Menkes disease was first raised after his death, serum copper and ceruloplasmin in serum were not measured. Unfortunately, no fibroblasts were available - only fixed and paraffin-embedded samples of brain, spleen and liver. The copper contents of the brain and the liver were lower than in an age-matched control. Fibroblast cultures from the mother, the maternal grandmother, and a maternal aunt of the index patient were analysed for 64Cu-uptake. All these females showed the uptake values expected for Menkes carriers, thus supporting the clinical suspicion of Menkes disease in the index patient. From the above-mentioned results it was highly likely that the index patient had suffered from Menkes disease. Adequate genetic counseling could thus be offered to the family, and in the next pregnancy a first trimester prenatal diagnosis was performed. PMID:3436089

  9. Speciation of human serum proteins based on trace metal mapping analysis by CIM monolithic disk column HPLC/ICP-MS in complement with off-line MALDI-TOF-MS analysis

    International Nuclear Information System (INIS)

    A multiply hyphenated HPLC/UV/ICP-MS system equipped with a fraction collection valve for subsequent MALDI-TOF-MS analysis was constructed, and its applicability to the speciation of metalloproteins in human serum was evaluated. A weak anion-exchange CIM monolithic disk column was employed for protein separation, in which gradient elution of proteins with volatile salts was carried out while taking into account the compatibility with ICP-MS instrument. As a result of optimization, representative serum proteins, such as γ-globulin (γGb), albumin (Ab), ceruloplasmin (Cp), transferrin (Tf), and α2-macroglobulin (α2Mgb), were separated on the disk column with a linear concentration gradient of CH3COONH4 in 20 mM Tris-HCl buffer (pH 7.4). These proteins, which were primarily assigned by UV absorption at 280 nm, were clearly identified with on-line multielement detection using ICP-MS as well as with off-line MALDI-TOF-MS analysis of each successive fraction collected. It was concluded that the present multiply hyphenated system will be useful for identifying proteins in biological samples. (author)

  10. Environmental temperature and stocking density effects on acute phase proteins, heat shock protein 70, circulating corticosterone and performance in broiler chickens

    Science.gov (United States)

    Najafi, Pardis; Zulkifli, Idrus; Amat Jajuli, Nurfarahin; Farjam, Abdoreza Soleimani; Ramiah, Suriya Kumari; Amir, Anna Aryani; O'Reily, Emily; Eckersall, David

    2015-11-01

    An experiment was conducted to determine the effect of different stocking densities on serum corticosterone (CORT), ovotransferrin (OVT), α1-acid glycoprotein (AGP) and ceruloplasmin (CP) concentrations, brain heat shock protein (HSP) 70 expression and performance in broiler chickens exposed to unheated and heated conditions. Day-old chicks were stocked at 0.100 m2/bird (low density (LD)) or 0.063 m2/bird (high density (HD)), in battery cages and housed in environmentally controlled rooms. From 21 to 35 days of age, birds from each stocking density group were exposed to either 24 or 32 °C. Growth performance was recorded during the heat treatment period, and blood and brain samples were collected to determine CORT, OVT, AGP, CP and HSP 70 levels on day 35. Heat treatment but not stocking density was detrimental to growth performance. There were significant temperature × density interactions for CORT, CP and OVT on day 35. Although HD elevated CORT, CP and OVT when compared to LD, the effects of the former were more obvious under heated condition. Both temperature and density had significant effect on AGP and HSP 70. In conclusion, irrespective of temperature, high stocking density was physiologically stressful to broiler chickens, as indicated by CORT, AGP, CP, OVT and HSP 70, but not detrimental to growth performance and survivability. As it was shown in the present study, AGP, CP and OVT could be useful biomarkers to determine the effect of overcrowding and high temperature on the welfare of broiler chickens.

  11. Enzimas no testículo de ratos em algumas condições experimentais

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    Helion Póvoa Junior

    1980-01-01

    Full Text Available Dosou-se a atividade da GOT, GPT e ceruloplasmina no testículo de ratos normais (a atividade testicular destas enzimas é relativamente baixa e injetados com cloreto de cádmio ( que lesa o testículo, assim como em animais com este órgão lesado e que se administrou testosterona, estradiol, progesterona e gonadotrofina coriônica. Pudemos observar alterações significativas das enzimas por ação destes diferentes hormônios. Infelizmente, como pouco se sabe sobre a função destas enzimas neste órgão, torna-se difícil interpretar os nossos achados.GOT, GPT and ceruloplasmin activities have been determined in testicle of normal rats. Low activities were found for every enzyme. In animals injected with cadmium chloride, there were significant alterations in enzyme activities. Accentuated alterations in enzyme activities were also found in animals with destroyed testicles after injection of several hormones (testosterone, estradiol, progesterone and chorionic gonadotrophin.

  12. Hypothalamic digoxin-mediated model for Parkinson's disease.

    Science.gov (United States)

    Kurup, Ravi Kumar; Kurup, Parameswara Achutha

    2003-04-01

    The isoprenoid pathway produces four key metabolites important in cellular function--digoxin (endogenous membrane Na(+)-K+ ATPase inhibitor), dolichol (important in N-glycosylation of proteins), ubiquinone (free-radical scavenger), and cholesterol (component of cellular membranes). This study assessed the changes in the isoprenoid pathway and the consequences of its dysfunction in Parkinson's disease (PD). There was an elevation in plasma HMG CoA reductase activity, serum digoxin and dolichol levels, and a reduction in serum magnesium, RBC membrane Na(+)-K+ ATPase activity, and serum ubiquinone levels. Serum tryptophan, serotonin, strychnine, nicotine, and quinolinic acid were elevated, while tyrosine, morphine, dopamine, and noradrenaline were decreased. The total serum glycosaminoglycans (GAG) and glycosaminoglycan fractions (except chondroitin sulphates and hyaluronic acid), the activity of GAG degrading enzymes, carbohydrate residues of serum glycoproteins, the activity of glycohydrolase-beta galactosidase, and serum glycolipids were elevated. HDL cholesterol was reduced and free fatty acids increased. The RBC membrane glycosaminoglycans, hexose and fucose residues of glycoproteins and cholesterol were reduced, while phospholipid was increased. The activity of all serum free-radical scavenging enzymes, concentration of glutathione, alpha tocopherol, iron binding capacity, and ceruloplasmin decreased significantly in PD, while the concentration of serum lipid peroxidation products and nitric oxide increased. A dysfunctional isoprenoid pathway and related cascade are important in the pathogenesis of Parkinson's disease. A hypothalamic digoxin mediated model for Parkinson's disease is also postulated. PMID:12856480

  13. Cerebellar ataxia of early onset

    International Nuclear Information System (INIS)

    Eight cases of childhood cerebellar ataxia were reported. All these cases showed chronic cerebellar ataxia with early onset, and the other diseases of cerebellum such as infections, neoplasms and storage diseases were excluded by clinical symptoms and laboratory findings including blood counts, blood chemistry, lactate, pyruvate, ceruloplasmine, urinalysis, serum immunoglobulins, amino acid analysis in blood and urine, CSF analysis, leukocyte lysosomal enzymes, MCV, EMG, EEG and brain X-CT. Two pairs of siblings were included in this study. The clinical diagnosis were cerebellar type (5), spinocerebellar type (1), one Marinesco-Sjoegren syndrome and undetermined type (1). The age of onset was 1 to 5 years. The chief complaint was motor developmental delay in 6 cases; among them 5 patients could walk alone at the ages of 2 to 3 years'. Mental retardation was observed in 7 cases and epilepsy in 2. TRH was effective in 5 cases. The MRI study revealed that the area of medial sagittal slice of the cerebellum was reduced significantly in all cases and also that of pons was reduced in 5 cases. Different from typical adult onset spinocerebellar degenerations, most of the present cases have achieved slow developmental milestones and the clinical course was not progressive. Genetic factors are suspected in the pathogenesis of this disease in some cases. (author)

  14. Urinary Biomarkers in the Assessment of Early Diabetic Nephropathy

    Science.gov (United States)

    Gluhovschi, Gheorghe; Petrica, Ligia; Timar, Romulus; Velciov, Silvia; Ionita, Ioana; Kaycsa, Adriana; Timar, Bogdan

    2016-01-01

    Diabetic nephropathy (DN) is a frequent and severe complication of diabetes mellitus (DM). Its diagnosis in incipient stages may allow prompt interventions and an improved prognosis. Towards this aim, biomarkers for detecting early DN can be used. Microalbuminuria has been proven a remarkably useful biomarker, being used for diagnosis of DN, for assessing its associated condition—mainly cardiovascular ones—and for monitoring its progression. New researches are pointing that some of these biomarkers (i.e., glomerular, tubular, inflammation markers, and biomarkers of oxidative stress) precede albuminuria in some patients. However, their usefulness is widely debated in the literature and has not yet led to the validation of a new “gold standard” biomarker for the early diagnosis of DN. Currently, microalbuminuria is an important biomarker for both glomerular and tubular injury. Other glomerular biomarkers (transferrin and ceruloplasmin) are under evaluation. Tubular biomarkers in DN seem to be of a paramount importance in the early diagnosis of DN since tubular lesions occur early. Additionally, biomarkers of inflammation, oxidative stress, podocyte biomarkers, and vascular biomarkers have been employed for assessing early DN. The purpose of this review is to provide an overview of the current biomarkers used for the diagnosis of early DN.

  15. Wilson disease with thrombocytopenia (case report).

    Science.gov (United States)

    Zhvania, M; Gogberashvili, K; Gagoshidze, M; Uberi, E

    2014-12-01

    We present an adolescent patient with WD accompanied with secondary amenorrhea, and thrombocytopenia. NK, a 14 year-old girl, had amenorrhea for 5 months despite having had regular menses for 2 years. An abdominal ultrasound scan revealed ascitis and some ovarian cysts. On physical examination: slight jaundice, edema of lower extremities, skin purpuric rash, enlarged abdomen, dry skin. She had no hepatomegaly and no splenomegaly. Breast and pubic hair development was concomitant with Tanner stage 4. There was performed laboratory and instrumental investigations. The patient was diagnosed as WD owing to the low level of ceruloplasmin, with increased level of copper in 24-hour urine excretion and in dry liver tissue. The needle biopsy of liver showed severe hepatocellular necrosis, inflammatory changes and fibrosis. The platelet count was found to be low with lack of increased number of megakaryocytes in the bone marrow aspiration suggesting the thrombocytopenia was not exclusively owing to hypersplenism. The absence of antithrombocyte and other autoimmune and viral antibodies excluded respectively the diagnosis of autoimmune thrombocytopenia, other autoimmune diseases and viral infections. Thus, we support the recommendation that adolescents with amenorrhea or children with thrombocytopenia without any obvious cause should be evaluated for WD, because the early detection and treatment of WD is capable of reversing described changes and restoring a normal liver function. PMID:25617103

  16. Oxidant stress, antioxidants and nitric oxide in traffic police of Hyderabad, India.

    Science.gov (United States)

    Suresh, Y; Sailaja Devi, M M; Manjari, V; Das, U N

    2000-08-01

    Exposure to environmental pollutants is known to be harmful to health, in general, and to lungs in particular. In this respect, traffic police are at particular risk due to the nature of their job, since they are exposed to emissions from the vehicles. Here, we show that in the traffic police of Hyderabad city, India, the plasma levels of lipid peroxides are high, whereas the concentrations of the nitric oxide are low. In addition, the levels of various antioxidants in the RBC lysate such as catalase, superoxide dismutase and glutathione peroxidase were found to be low with no significant alteration in plasma ceruloplasmin levels. These results suggest that exposure to air pollutants, a major portion of which is due to emissions from the vehicles, can increase oxidant stress, decrease the levels of antioxidants and nitric oxide. This imbalance in the oxidant/antioxidant system may lead to lung damage and is likely to cause respiratory problems in individuals exposed to air pollution. PMID:15092903

  17. Biological research for the radiation protection

    International Nuclear Information System (INIS)

    The work scope of 'Biological Research for the Radiation Protection' had contained the research about polyamine effect on cell death triggered ionizing radiation, H2O2 and toxic agents. In this paper, to elucidate the role of polyamines as mediator in lysosomal damage and stress(H2O2)- induced apoptosis, we utilized α-DiFluoroMethylOrnithine (DFMO), which inhibited ornithine decarboxylase and depleted intracellular putrescine, and investigated the effects of polyamine on the apoptosis caused by H2O2, ionizing radiation and paraquat. We also showed that MGBG, inhibitor of polyamine biosynthesis, treatment affected intracellular redox steady states, intracellular ROS levels and protein oxidation. Thereafter we also investigated whether MGBG may enhance the cytotoxic efficacy of tumor cells caused by ionizing radiation or H2O2 because such compounds are able to potentiate the cell-killing effects. In addition, ceruloplasmin and thioredoxin, possible antioxidant proteins, were shown to have protective effect on radiation- or H2O2(or chemicals)-induced macromolecular damage or cell death

  18. Portal copper transport in rats by albumin

    International Nuclear Information System (INIS)

    The distribution of newly absorbed copper among serum proteins obtained from the portal circulation of rats was examined by conventional and high-performance gel filtration chromatography, affinity chromatography, and Western blotting. Within 10-30 min after being administered by gavage or directly into the intestine, 67Cu and 64Cu, respectively, were recovered in the albumin fraction. By 8 h after administration of the radionuclides, virtually all of the radioactivity was found with ceruloplasmin. Affigel blue fractionation and subsequent Superose-6 chromatography further demonstrated that all of the copper in the albumin-containing fractions was in fact bound to this protein rather than high molecular weight moieties. Vascular perfusion of the isolated rat intestine, where 64Cu was infused into the lumen, showed that newly absorbed 64Cu in the vascular perfusate collected from the cannulated portal vein was associated with albumin. Uptake of radioactivity by isolated rat liver parenchymal cells from medium containing rat serum with 67Cu bound to albumin was demonstrated. In vitro binding of 64Cu to serum proteins that were transferred to nitrocellulose by Western blotting techniques showed that albumin is essentially the only protein that binds appreciable amounts of copper. The data suggest that albumin is the plasma protein that is responsible for the initial transport of copper after absorption

  19. Serum protein concentrations, including acute phase proteins, in calves experimentally infected with Salmonella Dublin

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    Daniela Gomes da Silva

    2011-07-01

    Full Text Available The aim of this study was to evaluate serum protein concentrations in calves experimentally inoculated with Salmonella Dublin. Twelve healthy 10 to 15-day-old Holstein calves were randomly allotted into two groups, control and infected with 10(8 CFU of Salmonella Dublin orally. The calves were subjected to physical evaluation and blood samples were collected shortly before administration of the bacteria and also 24, 48, 72, 96, 120 and 168 hours post-infection. The concentration of serum proteins was determined through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE. Thirty serum proteins ranging from molecular weight of 24,000 Da to molecular weight of 236,000 Da were detected. Serum concentrations of ceruloplasmin (125,000 Da, haptoglobin (45,000 Da, acid glycoprotein (40,000 Da and a 34,000 Da protein were significantly increased in the experimentally infected calves, when compared with their concentrations in the control animals. Therefore, this study showed that S. Dublin infection could lead to the increase of certain serum proteins in calves.

  20. Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice

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    Lina M. Ruiz

    2015-01-01

    Full Text Available Quercetin, a dietary flavonoid used as a food supplement, showed powerful antioxidant effects in different cellular models. However, recent in vitro and in vivo studies in mammals have suggested a prooxidant effect of quercetin and described an interaction with mitochondria causing an increase in O2∙- production, a decrease in ATP levels, and impairment of respiratory chain in liver tissue. Therefore, because of its dual actions, we studied the effect of quercetin in vivo to analyze heart mitochondrial function and erythropoiesis. Mice were injected with 50 mg/kg of quercetin for 15 days. Treatment with quercetin decreased body weight, serum insulin, and ceruloplasmin levels as compared with untreated mice. Along with an impaired antioxidant capacity in plasma, quercetin-treated mice showed a significant delay on erythropoiesis progression. Heart mitochondrial function was also impaired displaying more protein oxidation and less activity for IV, respectively, than no-treated mice. In addition, a significant reduction in the protein expression levels of Mitofusin 2 and Voltage-Dependent Anion Carrier was observed. All these results suggest that quercetin affects erythropoiesis and mitochondrial function and then its potential use as a dietary supplement should be reexamined.

  1. Menkes disease: importance of diagnosis with molecular analysis in the neonatal period

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    Larissa Sampaio de Athayde Costa

    2015-10-01

    Full Text Available Summary Menkes disease is a congenital disorder caused by changes in copper metabolism derived from mutations in the ATP7A gene. It is characterized by physical and neurological alterations. In the neonatal period, these alterations can be nonspecific, which makes early diagnosis a challenge. Diagnosis can be suspected when there are low levels of ceruloplasmin and serum copper. Molecular analysis confirms the diagnosis. Treatment is parenteral administration of copper histidine. We report a familial case with molecular confirmation. The proband had clinical and biochemical suspicious. Treatment with copper histidine was indicated, but initiated at the age of 2 months and 27 days only. He did not present improvements and died at 6 months. The mother became pregnant again, a male fetus was identified and copper histidine was manufactured during pregnancy. He was born healthy, biochemical markers were reduced and treatment was indicated. Molecular analysis was performed confirming mutation in both the mother and the proband, while the other son did not have mutation, so treatment was discontinued. We support the clinical relevance of molecular confirmation for the correct diagnosis and genetic counseling, once clinical findings in the neonatal period are nonspecific and early treatment with parenteral copper histidine must be indicated.

  2. Effects of Pregnancy and Lactation on Iron Metabolism in Rats

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    Guofen Gao

    2015-01-01

    Full Text Available In female, inadequate iron supply is a highly prevalent problem that often leads to iron-deficiency anemia. This study aimed to understand the effects of pregnancy and lactation on iron metabolism. Rats with different days of gestation and lactation were used to determine the variations in iron stores and serum iron level and the changes in expression of iron metabolism-related proteins, including ferritin, ferroportin 1 (FPN1, ceruloplasmin (Cp, divalent metal transporter 1 (DMT1, transferrin receptor 1 (TfR1, and the major iron-regulatory molecule—hepcidin. We found that iron stores decline dramatically at late-pregnancy period, and the low iron store status persists throughout the lactation period. The significantly increased FPN1 level in small intestine facilitates digestive iron absorption, which maintains the serum iron concentration at a near-normal level to meet the increase of iron requirements. Moreover, a significant decrease of hepcidin expression is observed during late-pregnancy and early-lactation stages, suggesting the important regulatory role that hepcidin plays in iron metabolism during pregnancy and lactation. These results are fundamental to the understanding of iron homeostasis during pregnancy and lactation and may provide experimental bases for future studies to identify key molecules expressed during these special periods that regulate the expression of hepcidin, to eventually improve the iron-deficiency status.

  3. New novel mutation of the ATP7B gene in a family with Wilson disease.

    Science.gov (United States)

    Lee, Jun-Young; Kim, Young-Hyun; Kim, Tae-Woo; Oh, Sun-Young; Kim, Dal-Sik; Shin, Byoung-Soo

    2012-02-15

    Wilson disease (WD) is an autosomal recessive disorder of copper metabolism. The WD gene codes for a copper transporting P-type ATPase (ATP7B) are located on chromosome 13q14.3. Mutation of this gene disrupts copper homeostasis, resulting in the accumulation of copper in the liver, brain, kidneys and corneas and copper toxication at these sites. Since the detection of the WD gene in 1993, approximately 300 disease-specific muations have been identified. We recently evaluated a Korean family with WD. The proband, a 17-year-old boy, visited our hospital due to abnormal behaviors including generalized slow movement, dysphagia, drooling and ataxia. Laboratory results revealed decreases in serum copper and ceruloplasmin and an increase in urinary excretion of copper. He had liver cirrhosis, brain lesions and Kayser-Fleischer corenal rings. Molecular genetic analysis of the ATP7B gene demonstrated that he was heterozygous for deletion mutation c.2697_2723del27 in exon 11. Further study of family members revealed that his father and younger brother had the same mutation. The c.2697_2723del27 deletion mutation in exon 11 has not yet been reported as a causative muation of WD and is an in-frame deletion not expected to lead to a frame shift. Therefore, we report a novel mutation of the ATP7B gene in a family with WD. PMID:22075048

  4. Antiinflammatory reactivity of copper(I)-thionein

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    Miesel, R.; Hartmann, H.J.; Weser, U. (Universitaet Tuebingen (Germany, F.R.))

    1990-10-01

    In unseparated human blood the reactivity of yeast copper (I)-thionein on TPA-activated polymorphonuclear leukocytes was evaluated and compared with low Mr copper chelates exerting Cu2Zn2 superoxide dismutase mimetic activity. Cu, 18 microM, in the form of Cu-thionein was sufficient to inhibit the superoxide production of activated human blood phagocytes by 50%. Furthermore, the scavenging of hydroxyl radicals and singlet oxygen by Cu(I)-thionein was determined, using the 2-deoxyribose fragmentation assay induced by decaying K3CrO8 and the NADPH oxidation caused by UVA illuminated psoralen, respectively. The inhibitory reactivity of Cu-thionein in both assays was compared with that of serum proteins including albumin, ceruloplasmin, transferrin, and ferritin. The galactosamine/endotoxin-induced hepatitis in male NMRI mice was used to evaluate the antiinflammatory reactivity of Cu-thionein in vivo. The serum copper, superoxide dismutase, and sorbitol dehydrogenase concentrations, as well as the activity of polymorphonuclear leukocytes in unseparated blood seemed most appropriate to quantify the protective capacity of Cu-thionein in the course of an oxidative stress-dependent liver injury. The intraperitoneal application of 32.5 mumols/kg thionein-Cu limited this damage to 45%.

  5. Modulation of oxidative stress and microinflammatory status by colloids in refractory dialytic hypotension

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    Rostoker Guy

    2011-10-01

    Full Text Available Abstract Background Intradialytic hypotension may adversely affect the outcome of chronic hemodialysis. Therapeutic albumin has powerful anti-oxidant and anti-inflammatory properties. We have recently shown that systematic colloid infusion during hemodialysis sessions improves hemodynamic parameters in most dialysis hypotension-prone patients unresponsive to usual of preventive measures. We postulated that frequent hypotensive episodes may lead to a noxious inflammatory response mediated by oxidative stress induced by ischemia-reperfusion. The aim of this study was therefore to analyze the effect of 20% albumin and 4% gelatin infusions on oxidative stress and microinflammatory status in hypotension-prone patients unresponsive to usual preventive measures. Methods Prospective cross-over study (lasting 20 weeks of routine infusion of 200 ml of 20% albumin versus 200 ml of 4% gelatin in 10 patients with refractory intradialytic hypotension. We analyzed the effect of 20% albumin and 4% gelatin on microinflammatory status, oxidative stress, serum nitrite and nitrate levels by analysis of variance. Results A significant decrease in serum ceruloplasmin and serum C3 was observed during the albumin period (p Conclusions We conclude that the improvement in microinflammatory status observed during colloid infusion in hypotension-prone dialysis patients may be related to a decrease in ischemia-reperfusion of noble organs, together with a specific reduction in oxidative stress by albumin. Trial registration ISRCTN 20957055

  6. Decreased T2 signal in the thalami may be a sign of lysosomal storage disease

    International Nuclear Information System (INIS)

    Lysosomal disorders are rare and are caused by genetically transmitted lysosomal enzyme deficiencies. A decreased T2 signal in the thalamus has occasionally been reported. Because the finding of bilateral abnormal signal intensity of the thalamus on T2-weighted images has not been systematically reviewed, and its value as a diagnostic tool critically evaluated, we carried out a systematic review of the literature. Articles in English with 30 trios of keywords were collected from PubMed. Exclusion criteria were lack of conventional T2-weighted images in the protocol and not being a human study. Finally, 111 articles were included. The thalamus was considered affected only if mentioned in the text or in the figure legends. Some 117 patients with various lysosomal diseases and five patients with ceruloplasmin deficiency were reported to have a bilateral decrease in T2 signal intensity. At least one article reported a bilateral decrease in signal intensity of the thalami on T2-weighted images in association with GM1 and GM2 gangliosidosis and with Krabbe's disease, aspartylglucosaminuria, mannosidosis, fucosidosis, and mucolipidosis IV. Furthermore, thalamic alteration was a consistent finding in several types of neuronal ceroid lipofuscinosis (NCL) including CLN1 (infantile NCL), CLN2 (classic late infantile NCL), CLN3 (juvenile NCL), CLN5 (Finnish variant late infantile NCL), and CLN7 (Turkish variant late infantile NCL). A decrease in T2 signal intensity in the thalami seems to be a sign of lysosomal disease. (orig.)

  7. Advancement in stationary phase for peptide separation helps in protein identification: application to atheroma plaque proteomics using nano-chip liquid chromatography and mass spectrometry.

    Science.gov (United States)

    Delporte, Cédric; Noyon, Caroline; Raynal, Pierre; Dufour, Damien; Nève, Jean; Abts, Frederic; Haex, Martin; Zouaoui Boudjeltia, Karim; Van Antwerpen, Pierre

    2015-03-13

    In the last decades, proteomics has largely progressed. Mass spectrometry and liquid chromatography (LC) are generally used in proteomics. These techniques enable proper separation of peptides and good identification and/or quantification of them. Later, nano-scaled liquid chromatography, improvements of mass spectrometry resolution and sensitivity brought huge advancements. Enhancements in chemistry of chromatographic columns also brought interesting results. In the present work, the potency of identification of proteins by different nano-chip columns was studied and compared with classical LC column. The present study was applied to cardiovascular field where proteomics has shown to be highly helpful in research of new biomarkers. Protein extracts from atheroma plaques were used and proteomics data were compared. Results show that fewer spectra were acquired by the mass spectrometer when nano-chip columns were used instead of the classical ones. However, approximately 40% more unique peptides were identified by the recently optimized chip named Polaris-HR-chip-3C18 column, and 20% more proteins were identified. This fact leads to the identification of more low-abundance proteins. Many of them are involved in atheroma plaque development such as apolipoproteins, ceruloplasmin, etc. In conclusion, present data shows that recent developments of nanoLC column chemistry and dimensions enabled the improved detection and identification of low-abundance proteins in atheroma plaques. Several of them are of major interest in the field of cardiovascular disease. PMID:25680550

  8. effect of interaction of praziquantel and an organophosphorus pesticide on some biochemical parameters in murine schistosomiasis mansoni

    International Nuclear Information System (INIS)

    in egypt schistosomiasis is still a major public health problem and PZQ is the drug of choice for its treatment, whereas DZN is globally used as pesticide for controlling pests. they adversely affect the environment . therefore, this investigation aimed to study the effect of 1/20 LD50 DZN given orally to healthy and S. mansoni infected mice, for 5 successive days up to 9 and 17 weeks coupled with a therapeutic dose (2 x 500 mg/kg b wt) of PZQ , 2 weeks before sacrificing . the results showed that the different treatments significantly reduced the activity of liver ALT. the highest inhibition was recorded in the infected-DZN - PZQ group at week 9. the maximum inhibition of brain ACHE activity was noticed in DZN-PZQ treated group after 9 and 17 weeks. although,the different treatments did not significantly affect the levels of plasma TP,ALB,G,and A/G ratio, significant differences were revealed from the G- fractions α1, α2, β1 , β2 and γ in addition to plasma ceruloplasmin

  9. Can antioxidants predispose to cancer recurrence?

    Institute of Scientific and Technical Information of China (English)

    Krishnananda Prabhu; Gummadi Maheshwar Reddy; Anjali Rao

    2010-01-01

    Objective:To measure and compare pre-and post-treatment levels of serum total glutathione-S-transferase (GST) in stage IV cervical cancer patients and erythrocytic glutathione (GSH) and malondialdehyde (MDA) and plasma ceruloplasmin (CP) and total GST in stage IV oral cancer patients and to correlate with the response to treatment during a two year follow-up period in respective cancer groups. Methods:Thirty-four biopsy-proven stage IV oral cancer and cervical cancer patients (n=17 in each group) who underwent same mode of treatment were chosen for this study. Erythrocytic MDA and GSH, CP and serum total GST were measured in all patients before the onset of treatment, and the GST level was only measured in cervical cancer patients after radiotherapy. The levels were compared with their respective prognosis in 2 years. Results:Oral cancer patients with higher pretreatment levels of GSH, CP and GST came with cancer recurrence within 2 years after the onset of treatment. Cervical cancer patients with higher post radiotherapy levels of serum total GST had higher recurrence tate. Conclusions:This study indicates there may be a role for these antioxidant parameters namely GST, GSH, CP in assessment of long term survival and prognosis of cancer patients.

  10. Review: Insights into molecular mechanisms of disease in neurodegeneration with brain iron accumulation: unifying theories.

    Science.gov (United States)

    Arber, C E; Li, A; Houlden, H; Wray, S

    2016-04-01

    Neurodegeneration with brain iron accumulation (NBIA) is a group of disorders characterized by dystonia, parkinsonism and spasticity. Iron accumulates in the basal ganglia and may be accompanied by Lewy bodies, axonal swellings and hyperphosphorylated tau depending on NBIA subtype. Mutations in 10 genes have been associated with NBIA that include Ceruloplasmin (Cp) and ferritin light chain (FTL), both directly involved in iron homeostasis, as well as Pantothenate Kinase 2 (PANK2), Phospholipase A2 group 6 (PLA2G6), Fatty acid hydroxylase 2 (FA2H), Coenzyme A synthase (COASY), C19orf12, WDR45 and DCAF17 (C2orf37). These genes are involved in seemingly unrelated cellular pathways, such as lipid metabolism, Coenzyme A synthesis and autophagy. A greater understanding of the cellular pathways that link these genes and the disease mechanisms leading to iron dyshomeostasis is needed. Additionally, the major overlap seen between NBIA and more common neurodegenerative diseases may highlight conserved disease processes. In this review, we will discuss clinical and pathological findings for each NBIA-related gene, discuss proposed disease mechanisms such as mitochondrial health, oxidative damage, autophagy/mitophagy and iron homeostasis, and speculate the potential overlap between NBIA subtypes. PMID:25870938

  11. Effect of vitamin C on copper retention in young men

    International Nuclear Information System (INIS)

    Previous work suggests that supplemental ascorbic acid (AA) may inhibit intestinal copper absorption by stabilizing the less absorbable cuprous state. The authors studied copper absorption in healthy men (age 19-32) fed a constant diet with different amounts of ascorbic acid supplements. The 6 men were confined to a metabolic unit for the entire 14 week study. The basal diet consisted of a 7 day rotating menu which provided an average of 2.1 mg Cu/d and was adequate in all other nutrients except AA (5 mg/d). The basal diet was supplemented with either zero, 60, or 600 mg of AA daily, added to grape juice and consumed at each meal. All feces were collected. Blood was taken weekly for monitoring AA and copper status. Copper absorption was determined by both balance and 65Cu stable isotope techniques. As determined by fecal Cu excretion, varying intakes of AA between 0.1 to 10 times the RDA had no significant effect on copper retention. This is consistent with the lack of change in serum ceruloplasmin and serum Cu throughout the study

  12. CT findings in Menkes' kinky hair disease

    International Nuclear Information System (INIS)

    Menkes' kinky hair disease is a disorder of copper metabolism with clinical features of motor and mental retardation, convulsive seizures and characteristic hair deformities. We followed CT findings in a case of kinky hair disease. The male infant weighting 3,240 g at birth after 37 weeks' gestation, developed convulsions at 3 months of age, suffered from pneumonia at 8 months and was admitted to our hospital because of frequent convulsions. On admission, he had rough, short, brown and curly hair. Serum copper and serum ceruloplasmin levels were low, and there was no response to oral loading of copper sulfate. CT scan revealed enlargement of ventricles, atrophy of cerebral hemispheres and spinal cord at 4 months, and subdural high density effusion at 9 months. A remarkable subdural hematoma that shows higher density than that of the effusions on CT scan appeared at 10 month, which was removed surgically. Xanthochromic effusion and subdural hematoma with septum were found during the opreation. It was concluded that these changes of CT scans were caused by reccurent minimal hemorrhage, and these hemorrhage was caused by rupture of bridging vein due to fragility of the veins and progressive brain atrophy. (author)

  13. Nutrititional Status Assessment of International Space Station Crew Members

    Science.gov (United States)

    Smith, S. M.; Zwart, S. R.; Block, G.; Rice, B. I.; Davis-Street, J. F.

    2005-01-01

    Defining optimal nutrient requirements is imperative to ensure crew health on long-duration space exploration missions. To date, nutrient requirement data have been extremely limited because of small sample sizes and difficulties associated with collecting biological samples. In this study, we examined changes in body composition, bone metabolism, hematology, general blood chemistry, and blood levels of selected vitamins and minerals after long-duration (128-195 d) space flight aboard the International Space Station. Crew members consumed an average of 80% of the recommended energy intakes, and on landing day their body weight had decreased (P=0.051). After flight, hematocrit was less, and serum femtin was greater than before flight (PSerum iron, ferritin saturation, and transferrin had decreased after flight. The finding that other acute-phase proteins, including ceruloplasmin, retinol binding protein, transthyretin, and albumin were not changed after flight suggests that the changes in iron metabolism may not be strictly due to an inflammatory response. Urinary 8- hydroxy-2'-deoxyguanosine concentration was greater and superoxide dismutase was less after flight, indicating that oxidative damage had increased (Pserum 25-hydroxyvitamin D was significantly decreased after flight (Pserum concentration of bone-specific alkaline phosphatase, was elevated only in crew members who landed in Russia, probably because of the longer time lapse between landing and sample collection. These data provide evidence that bone loss, compromised vitamin D status, and oxidative damage remain critical concerns for long-duration space flight.

  14. Responses of weanling pigs to dietary supplementation with vitamin C or carbadox.

    Science.gov (United States)

    Yen, J T; Pond, W G

    1984-01-01

    A 2 X 2 factorial arrangement with two levels (0, 660 ppm) of vitamin C and two levels (0, 55 ppm) of carbadox supplementation was used in two experiments with 112 crossbred pigs weaned between 4 and 5 wk of age. An 18% protein corn-soybean meal-oats-dried whey starter diet was used as the basal diet. Each diet was fed ad libitum for a 4-wk period to three replicates of four pigs in Exp. 1 and to four replicates of four pigs in Exp. 2. Vitamin C supplementation produced a significantly higher plasma vitamin C concentration in weanling pigs, but, contrary to results of our previous study, failed to improve average daily gain of the pigs. Daily gain was, however, improved significantly by carbadox supplementation. Carbadox also produced a significantly higher plasma vitamin C concentration in pigs after a 7-d lag period. Plasma Fe concentration of pigs was not affected by supplemental vitamin C, but was significantly higher in those fed carbadox-supplemented diets. Plasma ceruloplasmin concentration increased significantly in all treatment groups from the initial sampling period (d 0) to subsequent periods. No interactions between supplemental vitamin C and carbadox were observed in daily gain, feed efficiency and the measured plasma constituents. PMID:6698894

  15. Oral zinc supplementation decreases the serum iron concentration in healthy schoolchildren: a pilot study.

    Science.gov (United States)

    de Brito, Naira Josele Neves; Rocha, Érika Dantas; de Araújo Silva, Alfredo; Costa, João Batista Sousa; França, Mardone Cavalcante; das Graças Almeida, Maria; Brandão-Neto, José

    2014-09-01

    The recognized antagonistic actions between zinc and iron prompted us to study this subject in children. A convenience sample was used. Thirty healthy children between 8 and 9 years of age were studied with the aim of establishing the effect of a 3-mo oral zinc supplementation on iron status. Fifteen individuals were given a placebo (control group), and 15 were given 10 mg Zn/day (experimental group). Blood samples were collected at 0, 60, 120, 180 and 210 min after a 12-h overnight fast, before and after placebo or zinc supplementation. This supplementation was associated with significant improvements in energy, protein, fat, carbohydrate, fiber, calcium, iron, and zinc intake in accordance with the recommendations for age and sex. The basal serum zinc concentration significantly increased after oral zinc supplementation (p < 0.001). However, basal serum iron concentrations and area under the iron curves significantly decreased in the experimental group (p < 0.0001) and remained at the same level throughout the 210-min study. The values obtained for hemoglobin, mean corpuscular volume, ferritin, transferrin, transferrin saturation, ceruloplasmin and total protein were within normal reference ranges. In conclusion, the decrease in serum iron was likely due to the effects of chronic zinc administration, and the decrease in serum iron was not sufficient to cause anemia. PMID:25192026

  16. Antioxidative defense

    Directory of Open Access Journals (Sweden)

    Stevanović Jelka

    2011-01-01

    Full Text Available Free radicals occur constantly during metabolism and take part in numerous physiological processes, such as: intra-cellular and inter-cellular signalization, gene expression, removal of damaged or senescent cells, and control of the tone of blood vessels. However, there is an increased quantity of free radicals in situations of so-called oxidative stress, when they cause serious damage to cellular membranes (peroxidation of their lipids, damage of membrane proteins, and similar, to interior cellular protein molecules, as well as DNA molecules and carbohydrates. This is precisely why the organism has developed numerous mechanisms for removing free radicals and/or preventing their production. Some of these are enzyme-related and include superoxide-dismutase, catalase, glutathione-peroxidase, and others. Other, non-enzyme mechanisms, imply antioxidative activities of vitamins E and C, provitamin A, coenzyme Q, reduced glutation, and others. Since free radicals can leave the cell that has produced them and become dispersed throughout the body, in addition to antioxidative defense that functions within cellular structures, antioxidant extra-cellular defense has also been developed. This is comprised by: transferrin, lactoferrin, haptoglobin, hemopexin, ceruloplasmin, albumins, extra-cellular isoform SOD, extracellular glutathione-peroxidase, glucose, bilirubin, urates, and many other molecules.

  17. [Metabolic therapy and pulmonary disfunction in patients with obstetric sepsis].

    Science.gov (United States)

    Iakovlev, A Iu; Zaĭtsev, P M; Zubeev, P S; Mokrov, K B; Balandina, A V; Gushchina, N N; Kucherenko, V E

    2011-01-01

    The role of reamberin, a succinate-containing infusion preparation in correlation of pulmonary metabolic and respiratory disturbances in patients with obstetric puerperal sepsis was estimated. The prospective randomized study enrolled 43 patients with puerperal obstetric sepsis complicated by polyorganic deficiency (SOFA 8-10). Nineteen patients of the 1st group and 24 patients of the 2nd group were additionally treated with reamberin in a dose of 800 ml/day for 8 days. The venous and arterial difference by glucose, lactate, pyruvate, diene conjugates, malondialdehyde and ceruloplasmin was investigated. The blood gases were determined with the Ciba Corning 45 apparatus. Lower metabolic activity of the lungs with prevalence of the glucose anaerobic metabolism and lower activity of the intrapulmonary antioxidant protection were observed in the patients with obstetric sepsis. The use of reamberin in the complex therapy of obstetric sepsis promoted maintenance of the initial balance and anaeroibic and aerobic pulmonary metabolism, thus providing shorter terms of the decompensation and recovery of the lungs respiratory function. PMID:21913408

  18. Quantification of horse plasma proteins altered by xylazine using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry

    Science.gov (United States)

    MORI, Miwako; ICHIBANGASE, Tomoko; YAMASHITA, Shozo; KIJIMA-SUDA, Isao; KAWAHARA, Masahiro; IMAI, Kazuhiro

    2016-01-01

    ABSTRACT In the doping tests currently used in horse racing, prohibited substances or their metabolites are usually directly detected in urine or blood samples. However, despite their lasting pharmaceutical effects, some prohibited substances are rapidly eliminated from horse urine and blood, making them difficult to detect. Therefore, new indirect biomarkers for doping, such as plasma proteins that are increased by the prohibited substances, have recently attracted much attention. Here, a fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS) method was adopted for horse plasma proteomics analysis, in order to identify plasma proteins whose concentrations were altered in response to xylazine in Thoroughbred horses. Xylazine, which is rapidly absorbed and eliminated and has possibility of the change in the levels of plasma proteins, was selected as a model drug. Of the ten plasma proteins identified, four proteins, including three acute phase proteins (haptoglobin, ceruloplasmin, and α-2-macroglobulin-like), were significantly increased after xylazine administration. Therefore, our present approach might be useful in identifying indirect biomarkers of drug administration. PMID:26858580

  19. Serum lipids and proteins during treatment with a new oral contraceptive combination containing desogestrel.

    Science.gov (United States)

    Penttilä, I M; Bergink, E W; Holma, P; Hulkko, S; Makkonen, M; Pyörälä, T; Castrén, O

    1983-12-01

    The present study was carried out to measure lipid and protein levels in serum of healthy women during treatment with a new oral contraceptive combination containing 0.075 mg desogestrel (Org 2969, 17 alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol) plus 0.050 mg ethinyloestradiol per tablet. All 30 volunteers took 1 tablet daily for 21 consecutive days, followed by a tablet-free period of 7 days. Treatment lasted 3 months. At the end of treatment serum total cholesterol had increased by 0.26 mmol/l (5.0%), high-density lipoprotein-cholesterol by 0.22 mmol/l (15.2%) and triglycerides by 0.43 mmol/l (50%); the calculated low-density lipoprotein cholesterol had decreased by 0.16 mmol/l (4.9%). All lipid concentrations had returned to initial levels 2 months after treatment stopped. After 3 months treatment serum ceruloplasmin, cortisol-binding globulin capacity, sex-hormone-binding globulin capacity and thyroxine-binding globulin had significantly increased by 85.2, 133, 206 and 101%, respectively. All protein levels returned to normal 2 months after treatment stopped. The relationship between serum lipids and hormone-binding proteins has been discussed, as well as the significance of the high-density lipoprotein level with regard to contraceptive treatment. PMID:6232161

  20. Wilson's Disease

    Directory of Open Access Journals (Sweden)

    MH Amirian

    1986-07-01

    Full Text Available The reported case is a 9-year old female who was presented with following clinical signs and symptoms: Excessive salivation, difficulty in speaking and writing, deteriorating school performance, spasticity, dysarthria, dysphagia, tremor of the wrists and shoulders. The initial impression was Wilson's disease. Increased copper in the urine after 24 hour treatment with D-penicillamine, low serum copper level and low ceruloplasmin and presence of kayser-fleischer rings in ophthalmologic slit lamp examination confirmed the diagnosis of Wilson's disease and treatment with D-penicillamine was started. It is important to keep Wilson's disease in mind in evaluating patients with presentation similar to that of this patient. Wilson's disease is a recessively inherited defect of copper metabolism. In 70-805 of cases, the presenting clinical manifestations are hepatic or neurological. kayser-fleischer ring is present in 75% of children who present with hepatic symptoms and in all children with neurological changes. The histologic appearance of liver biopsy in Wilson's disease is not pathognomonic. The early diagnosis and treatment of Wilson's disease is extremely important, as effective therapy is available. D-penicillamine is the most effective drug in treating Wilson's disease.

  1. Wilson's disease with peculiar MR-CT imaging

    International Nuclear Information System (INIS)

    A 15-year-old boy was admitted to our hospital complaining of progressive right hemiparesis and dysarthria. The diagnosis of Wilson's disease was established by Kayser-Fleischer rings and biochemical examinations, including low plasma copper and ceruloplasmin concertration. A paternal cousin was known to be Wilson's disease and died at 10 years of age. The brain CT scan demonstrated low density areas in bilateral thalami and lentiform nuclei. In addition, bilateral frontal lobes, and left temporal lobe showed a large low density area. The brain MR-CT demonstrated long T1 (dark) area on IR scans and long T2 (light) area on SE scans in bilateral thalami, and lentiform nuclei. The gross leisions in bilateral frontal lobes and left temporal lobe were imaged as dark areas on IR as well as on SE scans. We suggested that the latter may reflect the formation of gross cavities corresponding to the loss of excessive amount of white matter, rarely reported in the autopsy cases in the literature. (author)

  2. Bilateral symmetrical low density areas in the striatum

    International Nuclear Information System (INIS)

    A 10-year-old boy, who showed low density areas at bilateral striatal portion on brain CT, was reported. Characteristic clinical features were summarized as follows: 1. Onset in childhood (3 years old), 2. gait disturbance, dysarthria, involuntaly movement such as choreoathetosis and dystonia, 3. mild mental retardation (IQ 70), and 4. slowly progressive course over several years. Family history was unremarkable. His parents were not consanguineous. He was well until 3 years old, when he developed gait disturbance. At the age of 4, CT showed hypodensity lesions in the bilateral putamens, and right caudate was involved at 7, followed by bilateral caudate involvements at 10. Laboratory findings including blood lactate, pyruvate, serum copper, ceruloplasmin, aminoacids, urine and CSF catecholamines were within normal limits. TRH and thiamine therapies were ineffective L-dopa was slightly effective in movements, but symptoms were slowly progressive. We reviewed fourteen reported cases which were similar to our case in their onset, symptoms, clinical course and CT findings. Although the etiology was unknown, this case is possibly a new disease entity. (author)

  3. Development of technology for biological dosimetry

    International Nuclear Information System (INIS)

    Adult male rats were treated a single, whole body exposure to a dose of 0.1, 0.5, 1, 2, 3, 5, 7 and 9 Gy. The animal were sacrificed 6, 24, 48, 72, 96 and 212 hours following exposure. Enzyme activity changes such as alkaline phosphatase, GOT, creating kinase, MDH and LDH in rat serum as biochemical indicators useful for evaluating radiation exposure were measured. An competitive enzyme linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum after gamma-irradiation. Rat alpha-2 macroglobulin, alpha-1 acid glycoprotein, haptoglobin, ceruloplasmin, C-reactive protein and alpha-1 antitrypsin were purified from the plasma of turpentine treated rats. In vitro somatic mutation induced by gamma-irradiation and pentachlorophenol (PCP) which is representative of chemical pollutant was measured at the hypoxanthine-guanine phosphorybosyl transferase (HPRT) locus in human T-lymphocytes by a cell cloning assay. Mutant cells were selected by their ability to form a clone in the presence of purine analogue 6-thioguanine. Reverse transcriptase/polymerase chain reaction technique was needed for the mutation spectrum to discriminate combined exposure to radiation and chemicals. (author). 98 refs., 7 tabs., 47 figs

  4. Anti-inflammatory activity of copper-protein complexes with reference to rheumatoid arthritis

    International Nuclear Information System (INIS)

    Work was carried out on the serum of 100 healthy subjects and hundred Rheumatoid Arthritus patients by electrophoresis and atomic absorption model Hitachi-180-80. The different proteins fractions separated by electrophoresis were subjected for estimation of Cu-Concentration. It was found that only 5% copper is present in albumin which remains constant in normal and Rheumatoid Arthritus patients. The other important group of proteins were a2-globulins, in which Cu concentration was found high in Rheumatoid Arthritus patients as compared to normal subject. There was no copper detected in beta and gamma globulins. The study can be used for diagnostic purposes. Copper complexes of alpha 2-Globulins were found anti inflammatory in nature and this quality of self defense can bring revolution in the history of all infectious diseases including Rheumatoid Arthritus simply by separating the copper complexes of alpha 2-globulins (most probably ceruloplasmin) from the blood of donors and injecting into the body of patients. It is possible by this method that the disease-Rheumatoid Arthritus may be eradicated completely. These complexes have also shown antibiotic activity in gynae patients. (author)

  5. [The effect of dietotherapy on the clinico-immunological indices of rheumatoid arthritis patients].

    Science.gov (United States)

    Sharafetdinov, Kh Kh; Denisov, L N; Samsonov, G R; Pokrovskaia, G R; Voĭtko, N E

    1990-01-01

    The main requirements for the dietotherapy of rheumatoid arthritis (RA) patients have been formulated, and for the first time a pathogenetically balanced diet has been developed that produces antiinflammatory and immunocorrective effects. The effectiveness of the dietotherapy included into the combined treatment was studied in 41 patients with classic and proved RA. The authors have studied the influence of the dietotherapy on the clinical signs characterizing the activity of inflammation in the joints, and laboratory immunological parameters (IgG, IgM, IgA, IgD, the complement components: C1-inhibitor, C3, C4, C5, C9; haptoglobin, orrhozomucoid, transferrin, ceruloplasmin, alpha 1-antitrypsin, alpha 2-macroglobulin, prealbumin). The results of the study have shown that the dietotherapy is not only conducive to the improvement of the clinical picture of the disease, that permits diminishing the amount of nonsteroid antiinflammatory drugs used, but also produces a favourable effect on the RA patients' immunological state. It has been concluded that inclusion of the dietotherapy into the combined treatment of RA patients is advisable. PMID:2346005

  6. Urine proteomic profiling of uranium nephrotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Malard, V.; Gaillard, J.C.; Sage, N. [CEA, DSV, IBEB, SBTN, Laboratoire de Biochimie des Systemes Perturbes (LBSP), Bagnols-sur-Ceze, F-30207 (France); Berenguer, F. [CEA, DSV, IBEB, SBTN, Laboratoire d' Etude des Proteines Cibles (LEPC), Bagnols-sur-Ceze, F-30207 (France); Quemeneur, E. [CEA, DSV, IBEB, SBTN, Bagnols-sur-Ceze, F-30207 (France)

    2009-07-01

    Uranium is used in many chemical forms in civilian and military industries and is a known nephro-toxicant. A key issue in monitoring occupational exposure is to be able to evaluate the potential damage to the body, particularly the kidney. In this study we used innovative proteomic techniques to analyse urinary protein modulation associated with acute uranium exposure in rats. Given that the rat urinary proteome has rarely been studied, we first identified 102 different proteins in normal urine, expanding the current proteome data set for this central animal in toxicology. Rats were exposed intravenously to uranyl nitrate at 2.5 and 5 mg/kg and samples were collected 24 h later. Using two complementary proteomic methods, a classic 2-DE approach and semi-quantitative SDS-PAGE-LC-MS/MS, 14 modulated proteins (7 with increased levels and 7 with decreased levels) were identified in urine after uranium exposure. Modulation of three of them was confirmed by western blot. Some of the modulated proteins corresponded to proteins already described in case of nephrotoxicity, and indicated a loss of glomerular permeability (albumin, alpha-1-anti-proteinase, sero-transferrin). Others revealed tubular damage, such as EGF and vitamin D-binding protein. A third category included proteins never described in urine as being associated with metal stress, such as ceruloplasmin. Urinary proteomics is thus a valuable tool to profile uranium toxicity non-invasively and could be very useful in follow-up in case of accidental exposure to uranium. (authors)

  7. BIOCHEMICAL MECHANISMS OF MIXED EFFECT OF ELECTROMAGNETIC RADIATION AND LOW POSITIVE TEMPERATURE ON ANIMALS’ ORGANISM

    Directory of Open Access Journals (Sweden)

    Litovchenko O.L.

    2015-05-01

    Full Text Available At present, biochemical mechanisms of mixed effects of electromagnetic radiation (EMR and cold on the body are not adequately studied, so this problem is urgent for modern medicine. Purpose of study. Establishing pathognomonic criteria and biochemical mechanisms of adverse effect of EMR on the organism of laboratory animals in conditions of cold stress. Materials and methods. The laboratory subacute experiment was carried out on mature white male rats of WAG line, weighing 190-220 g for 1 month. The animals were divided into 4 groups of 10 animals in each group. The first group was subjected to the isolated action of electromagnetic radiation (frequency 70 kHz, tension 600 V/m at a comfortable air temperature of 25 ± 2 ° C. The second group was subjected to the mixed action of EMR and low temperature 4 ± 2°C. The third group served as a control with regard to the first group, and the fourth group - with regard to the second, at air temperature of 25 ± 2°C. Expositions were carried out 5 times a week (for 4:00 every day. To identify changes in biochemical parameters studied during the experiments, blood sampling was performed at the stages of 5, 15, 30 days and urine sampling – at the stages of 15, 30 days in dynamics. Blood serum was used as biomaterial. It was determined the content of malondialdehyde (MDA, conjugated diene, content of SH-groups, superoxide dismutase, ceruloplasmin, cholesterol, high density lipoprotein, low density lipoprotein, very low density lipoprotein (VLDL, triglycerides, atherogenic index was determined, the level of urea, alkaline phosphatase, acid phosphatase, content of chlorides, calcium, magnesium, phosphorus, total protein, glucose, and catalase activity. Renal function was studied by the content of creatinine, cholinesterase, urea, uric acid, chlorides, potassium, sodium, calcium, phosphorus and glucose in urine. Results and discussion. The findings showed that the isolated action of EMR only led to a

  8. Metabolizable protein supply modulated the acute-phase response following vaccination of beef steers.

    Science.gov (United States)

    Moriel, P; Arthington, J D

    2013-12-01

    Our objective was to evaluate the effects of MP supply, through RUP supplementation, on the acute-phase response of beef steers following vaccination. On d 0, Brangus-crossbred steers (n = 24; 173 ± 31 kg; 175 ± 16 d of age) were randomly assigned to receive 1 of 3 isocaloric diets formulated to provide 85, 100, and 115% of the daily MP requirements of a beef steer gaining 0.66 kg of BW daily. Diets were limit-fed at 1.8% of BW (DM basis) and individually provided to steers once daily (0800 h) from d 0 to 29. Steers were weighed on d 0 and 29, following a 12-h period of feed and water withdrawal. On d 7, steers were vaccinated against Mannheimia haemolytica (OneShot, Pfizer), and blood samples were collected on d 0, 7, 8, 10, 14, 21, and 30. Plasma metabolites were analyzed as repeated measures using the MIXED procedure of SAS. Final BW and ADG were similar (P ≥ 0.50) among treatments (mean = 184 ± 9 kg and 0.5 ± 0.08 kg/d, respectively). Effects of time were detected (P < 0.01) for plasma concentrations of all acute-phase proteins, which peaked between d 7 to 14, returning to baseline concentrations by d 29. Treatment effects were not detected (P ≥ 0.19) for plasma concentrations of acid-soluble protein, albumin, fibrinogen, IGF-1 and serum amyloid-A. Plasma concentrations of total protein (TP) and plasma urea nitrogen (PUN) increased (P ≤ 0.05) with increasing supply of MP (87.1, 89.6, and 90.1 ± 1.09 mg TP/mL and 6.1, 8.3, and 10.3 ± 0.41 mg PUN/dL for 85, 100, and 115% MP steers, respectively). From d 10 to 29, steers provided 115% MP had less (P < 0.001) plasma concentrations of ceruloplasmin than steers fed 85 and 100% MP, which had similar plasma ceruloplasmin concentrations. On d 14, plasma concentrations of haptoglobin were greatest (P ≤ 0.06) for steers fed 115% MP, intermediate for 100% MP, and least for 85% MP (0.98, 0.71 and 0.44 ± 0.099 mg/mL, respectively). On d 10, plasma concentrations of creatinine were greater (P = 0.01) for steers

  9. Plasma protein profiling of mild cognitive impairment and Alzheimer's disease across two independent cohorts.

    Science.gov (United States)

    Muenchhoff, Julia; Poljak, Anne; Song, Fei; Raftery, Mark; Brodaty, Henry; Duncan, Mark; McEvoy, Mark; Attia, John; Schofield, Peter W; Sachdev, Perminder S

    2015-01-01

    To unlock the full potential of disease modifying treatments, it is essential to develop early biomarkers for Alzheimer's disease (AD). For practical reasons, blood-based markers that could provide a signal at the stage of mild cognitive impairment (MCI) or even earlier would be ideal. Using the proteomic approach of isobaric tagging for relative and absolute quantitation (iTRAQ), we compared the plasma protein profiles of MCI, AD, and cognitively normal control subjects from two independent cohorts: the Sydney Memory and Ageing Study (261 MCI subjects, 24 AD subjects, 411 controls) and the Hunter Community Study (180 MCI subjects, 153 controls). The objective was to identify any proteins that are differentially abundant in MCI and AD plasma in both cohorts, since they might be of interest as potential biomarkers, or could help direct future mechanistic studies. Proteins representative of biological processes relevant to AD pathology, such as the complement system, the coagulation cascade, lipid metabolism, and metal and vitamin D and E transport, were found to differ in abundance in MCI. In particular, levels of complement regulators C1 inhibitor and factor H, fibronectin, ceruloplasmin, and vitamin D-binding protein were significantly decreased in MCI participants from both cohorts. Several apolipoproteins, including apolipoprotein AIV, B-100, and H were also significantly decreased in MCI. Most of these proteins have previously been reported as potential biomarkers for AD; however, we show for the first time that a significant decrease in plasma levels of two potential biomarkers (fibronectin and C1 inhibitor) is evident at the MCI stage. PMID:25159666

  10. ANAESTHETIC MANAGEMENT OF A PATIENT WITH WILSON’S DISEASE POSTED FOR EMERGENCY LSCS: A CASE REPORT

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    Kousalya

    2015-06-01

    Full Text Available Wilson’s disease (WD, or hepatolenticular degeneration, is a rare autosomal recessive disorder with a prevalence of 1:50,000 - 1:100,000 live births . 1 The genetic disorder causes a reduction in the synthesis of the copper transporting protein ceruloplasmin. Clinical presentation may vary from asymptomatic patients to those presenting with fulminant liver disease or neurological symptoms or psychiatric illness. The course of events in a pregnant patient depends upon the preconception status of the disease spectrum. The neuro - psychiatric illness and hepatorenal involvement in antenatal period may get worsene d. The risk of ascitis, Gatrointestinal bleeding and Varicial rupture is increased in pregnant women with pre - existing cirrhosis. Elevated serum copper levels are associated with post - partum depression (PPD. Copper accumulation may lead to erythrocyte hemolysis resulting in Coombs negative haemolytic anaemia (10 – 15%. There is also an increased risk of Hypertensive disorders in Pregnancy, HELLP syndrome and placental abruption. The disease status at the time of presentation for surgery should determine the mode of Anaesthesia, intraoperative management and post - operative care. Hepatic dysfunction can cause delayed recovery with General anaesthesia. Pre - existing neuro psychological problems may get aggravated by the hypnotic and sedative drugs. Excessive copper may interfere with neuromuscular transmission. Pre - existing neurological deficiency must be documented before attempting an Epidural or a subarachnoid block. We report a case of a primigravida with previously diagnosed Wilson’s disease initially presented with neurological symptoms and muscular weakness in the lower limbs. Though treated for 3yrs with trientene and zinc, she was on irregular treatment when posted for Emergency Caesarean section. After a proper Preanesthetic evaluation of the patient, LSCS was conducted under subarachnoid block. A good intra operative

  11. [Neuropsychiatric disorders associated with streptococci: a case report].

    Science.gov (United States)

    Fernández Ibieta, M; Ramos Amador, J T; Auñón Martínc, I; Marín, M A; González Tomé, M I; Simón de Las Heras, R

    2005-05-01

    Compulsive neuropsychiatric disorders are common in children and the most frequent is Gilles de la Tourette syndrome. Recently, a new disease has been described: the PANDAS syndrome (pediatric autoimmune neuropsychiatric disorders associated with streptococci). The etiology of this syndrome is uncertain but it has been associated with recent group A streptococcal infection (GAS). After an episode of pharyngitis, a boy aged 3 years and 9 months showed abrupt onset of a variety of neurobehavioral problems such as tics (consisting of elevation of the head and ipsilateral shoulder, winking, and grimaces) and compulsions (such as repeatedly hitting objects). A complete biochemical study was performed, including Cu and ceruloplasmin (which had normal values), antistreptolysin O (ASLO) and anti-DNAse (showing elevated values). Pharyngeal culture revealed GAS. The child was initially treated with valproic acid, but his subsequent improvement coincided with penicillin treatment for 10 days. Three months afterwards, ASLO values were reduced and at the 6-month follow-up the improvement was maintained even after suspension of valproic acid. Immunohistochemical studies were requested. PANDAS syndrome was first described in 1998 in a group of children who presented 1) obsessive compulsive disorders and/or tics, 2) episodic course with abrupt exacerbations, 3) abnormal results of neurologic examination (choreiform movements), and 4) temporal relation between GAS infection and onset of symptoms. The etiology of this syndrome is unclear, and it has been postulated that certain streptococcal antigens trigger antibodies which, through a process of molecular mimicry, cross-react with epitopes on the basal ganglia of susceptible hosts, such as the B8/17 antigen, among others. Current recommendations include penicillin treatment of each exacerbation with positive throat culture, and more aggressive therapies (intravenous immunoglobulin or plasmapheresis) when symptoms are severe. We

  12. Metallothionein and antioxidant enzymes in Long-Evans Cinnamon rats treated with zinc

    Energy Technology Data Exchange (ETDEWEB)

    Medici, Valentina; Sturniolo, Giacomo Carlo; D' Inca, Renata [Department of Surgical and Gastroenterological Sciences, University of Padua, Padua (Italy); Santon, Alessandro; Giannetto, Sabrina; Albergoni, Vincenzo; Irato, Paola [Department of Biology, University of Padua, via U. Bassi 58/B, 35131 Padua (Italy)

    2002-09-01

    The Long-Evans Cinnamon (LEC) rat is a mutant animal model for Wilson's disease. It is known that an abnormal accumulation of Cu and Fe in the liver and low concentrations of both ceruloplasmin and Cu in the serum occur in these rats. The accumulation of Cu is explained by the defective expression of the Cu-transporting P-type ATPase gene, homologous to the gene for Wilson's disease (ATP7B). The aim of this work was to clarify the action mechanism of Zn, and to verify the role that this metal plays in LEC rats in short-term treatment experiments (1 and 2 weeks) on concentrations of Cu, Zn, Fe, metallothionein (MT), 8-hydroxy-2'-deoxyguanosine (oh{sup 8}dG) and on the activity of antioxidant enzymes. It is well known that Zn induces MT and has the ability to prevent redox-active metals, Cu and Fe, binding to and causing oxidative damage at active sites of Zn metalloenzymes and nonspecific binding sites on proteins. Zn administration reduces Cu and Fe transport from mucosal to serosal intestinal sides through competitive mechanisms. Our findings show that treatment with zinc acetate increases tissue Zn and MT contents and decreases Cu and Fe concentrations in the liver and kidneys, even if hepatic Zn and MT concentrations decrease with treatment period. Induction of MT synthesis by Zn contributes to the reduction in free radicals produced by Cu and Fe. We also observed that the superoxide dismutase (SOD)activity in liver decreases with treatment duration in association with the Cu and Fe liver decrease. However, the SOD activity in kidney increases in untreated rats at 2 weeks relative to those untreated for 1 week. (orig.)

  13. Free Radicals and Antioxidant Status in Protein Energy Malnutrition

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    M. Khare

    2014-01-01

    Full Text Available Background/Objectives. The aim of this study was to evaluate oxidant and antioxidant status in children with different grades of Protein Energy Malnutrition (PEM. Subjects/Methods. A total of two hundred fifty (250 children (age range: 6 months to 5 years living in eastern UP, India, were recruited. One hundred and ninety-three (193 of these children had different grades of PEM (sixty-five (65 children belong to mild, sixty (60 to moderate, and sixty-eight (68 to severe group. Grading in group was done after standardization in weight and height measurements. Fifty-seven (57 children who are age and and sex matched, healthy, and well-nourished were recruited from the local community and used as controls after checking their protein status (clinical nutritional status with height and weight standardization. Redox homeostasis was assessed using spectrophotometric/colorimetric methods. Results. In our study, erythrocyte glutathione (GSH, plasma Cu, Zn-superoxide dismutase (Cu,Zn-SOD,EC 1.15.1.1, ceruloplasmin (Cp, and ascorbic acid were significantly (P<0.001 more decreased in children with malnutrition than controls. Plasma malondialdehyde (MDA, and protein carbonyl (PC were significantly (P<0.001 raised in cases as compared to controls. Conclusion. Stress is created as a result of PEM which is responsible for the overproduction of reactive oxygen species (ROSs. These ROSs will lead to membrane oxidation and thus an increase in lipid peroxidation byproducts such as MDA and protein oxidation byproducts such as PC mainly. Decrease in level of antioxidants suggests an increased defense against oxidant damage. Changes in oxidant and antioxidant levels may be responsible for grading in PEM.

  14. Oxidative Stress and Cell Apoptosis in Caprine Liver Induced by Molybdenum and Cadmium in Combination.

    Science.gov (United States)

    Yang, Fan; Zhang, Caiying; Zhuang, Yu; Gu, Xiaolong; Xiao, Qingyang; Guo, Xiaoquan; Hu, Guoliang; Cao, Huabin

    2016-09-01

    To investigate the effects of co-exposure to molybdenum (Mo) and cadmium (Cd) on oxidative stress and cell apoptosis in caprine livers, 36 Boer goats were randomly divided into four groups with nine goats in each group. Three groups were randomly assigned with one of three oral treatments of CdCl2 (0.5 mg Cd kg(-1)·BW) and [(NH4)6Mo7O24·4H2O] (15 mg Mo kg(-1)·BW, 30 mg Mo kg(-1)·BW, 45 mg Mo kg(-1)·BW), while the control group received deionized water. Liver tissues on days 0, 25, and 50 were subjected to determine antioxidant activity indexes and the messenger RNA (mRNA) expression levels of ceruloplasmin (CP), cysteinyl aspartate-specific proteinase-3 (caspase-3), second mitochondria-derived activator of caspases (Smac), and cytochrome-C (Cyt-C) genes. The results showed that significant reductions were observed in total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activities (P < 0.05), while activities or contents of malondialdehyde (MDA), nitric oxide (NO), and nitric oxide synthase (NOS) were increased (P < 0.05). The mRNA expression levels of CP, caspase-3, Smac, and Cyt-C genes were upregulated (P < 0.05). In addition, histopathological lesions showed different degrees of vacuolar degeneration and edematous and mitochondrial swelling. The results suggest that co-exposure to Mo and Cd could induce oxidative stress and cell apoptosis possibly associated with mitochondrial intrinsic pathway in goat liver and show possible synergistic effects between the two elements. PMID:26883837

  15. Searching for early breast cancer biomarkers by serum protein profiling of pre-diagnostic serum; a nested case-control study

    International Nuclear Information System (INIS)

    Serum protein profiles have been investigated frequently to discover early biomarkers for breast cancer. So far, these studies used biological samples collected at or after diagnosis. This may limit these studies' value in the search for cancer biomarkers because of the often advanced tumor stage, and consequently risk of reverse causality. We present for the first time pre-diagnostic serum protein profiles in relation to breast cancer, using the Prospect-EPIC (European Prospective Investigation into Cancer and nutrition) cohort. In a nested case-control design we compared 68 women diagnosed with breast cancer within three years after enrollment, with 68 matched controls for differences in serum protein profiles. All samples were analyzed with SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry). In a subset of 20 case-control pairs, the serum proteome was identified and relatively quantified using isobaric Tags for Relative and Absolute Quantification (iTRAQ) and online two-dimensional nano-liquid chromatography coupled with tandem MS (2D-nanoLC-MS/MS). Two SELDI-TOF MS peaks with m/z 3323 and 8939, which probably represent doubly charged apolipoprotein C-I and C3a des-arginine anaphylatoxin (C3adesArg), were higher in pre-diagnostic breast cancer serum (p = 0.02 and p = 0.06, respectively). With 2D-nanoLC-MS/MS, afamin, apolipoprotein E and isoform 1 of inter-alpha trypsin inhibitor heavy chain H4 (ITIH4) were found to be higher in pre-diagnostic breast cancer (p < 0.05), while alpha-2-macroglobulin and ceruloplasmin were lower (p < 0.05). C3adesArg and ITIH4 have previously been related to the presence of symptomatic and/or mammographically detectable breast cancer. We show that serum protein profiles are already altered up to three years before breast cancer detection

  16. Whole-body cryostimulation--potential beneficial treatment for improving antioxidant capacity in healthy men--significance of the number of sessions.

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    Anna Lubkowska

    Full Text Available It is claimed that WBC (whole-body cryotherapy enhances the resistance of the human body, also thanks to the beneficial effect on the antioxidant system. Accordingly, this research aimed to evaluate the effect of a series of whole-body cryostimulations on the level of non-enzymatic antioxidants and the activity of antioxidant enzymes in healthy men. The study was carried out on 30 young and healthy men aged 27.8±6.1 years with average body mass index and peak oxygen consumption (46.34±6.15 ml kg(-1 •min(-1. The participants were daily exposed for 3 minutes to cryogenic temperatures (-130°C. Blood samples were obtained in the morning before cryostimulation, again 30 min after exposure and the following day in the morning, during the 1(st, 10(th and 20(th session. Analysis concerned changes in plasma concentrations of total protein, albumin, glucose, uric acid and ceruloplasmin, and the most important components of the antioxidant system in red blood cells: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, reduced and oxidized glutathione. To assess the oxidative stress level the 8-isoprostane concentration in plasma was measured. The obtained results indicate that cryogenic temperatures in repeated daily treatments result in changes in the peroxidant and antioxidant status. These changes seem to depend on the number of cryostimulations. After 20 daily treatments there was an increase in SOD, SOD:CAT ratio, a decrease in the concentration of reduced and oxidized glutathione and in the activity of GPx. It could be possible that differences in the activity of GSSG-R after 20 treatments depended on the body mass index of participants.

  17. [CHANGES IN LIFESTYLE FACTORS AFFECT THE LEVELS OF NEUROPEPTIDES, INVOLVED IN THE CONTROL OF EATING BEHAVIOR, INSULIN RESISTANCE AND LEVEL OF CHRONIC SYSTEMIC INFLAMMATION IN YOUNG OVERWEIGHT PERSONS].

    Science.gov (United States)

    Shevchenko, Yu; Mamontova, T; Baranova, A; Vesnina, L; Kaidashev, I

    2015-11-01

    The continuous rise of overweight and obesity is a major concern for present and future healthcare management. The aim of our study was to evaluate the influence of lifestyle changes on central regulatory mechanisms of maintaining energy homeostasis, insulin resistance and chronic systemic inflammation in 18-25 year old overweight patients. The anthropometric measurements were done for each patient, including body weight, height, waist circumference (WC), hip circumference (HC), waist to hip ratio, and body mass index (BMI). Patients were divided into two groups according to their BMI. 20 males and 21 females with BMI > 25 kg/m2 were included in the first, study, group. The second, control, group consisted of 16 males and 11 females with BMI - 18.5 - 24.9 kg/m 2. Lipid profile, fasting glucose and insulin levels, HOMA-IR, TNF-α, ceruloplasmin, neuropeptides Agouti-related protein - AgRP and kokain- and amphetamine-mediated transcript - CART were examined at the beginning of the study (Day 1) and after 10 week treatment period (Day 70). During the 10 week treatment period, people in the study group underwent physical exercise programs of 40-60 minute duration three times per week, and brisk walking during the remaining days. The mean calorie expenditure during the exercise was 500 kcal/day. Each patient in the study group was given balanced, healthy diet with 20% calorie restriction from baseline diet. Our study reveled that negative energy balance caused a statistically significant decrease in AgRP, reduction of visceral fat stores, increase of HDL, reduction of TNF-α and dissolution of insulin resistance signs. Significant but not statistically significant reduction of CART level was observed after increased physical activity program and calorie restriction diet, which might play an important role in the mechanism of decreasing weight. However, further investigations are required in order to determine its place in body weight regulation. PMID:26656551

  18. Effects of hemochromatosis and transferrin gene mutations on peripheral iron dyshomeostasis in Mild Cognitive Impairment and Alzheimer’s and Parkinson’s diseases

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    Stefania eMariani

    2013-08-01

    Full Text Available Deregulation of iron metabolism has been observed in patients with neurodegenerative diseases. We have carried out a molecular analysis investigating the interaction between iron specific gene variants [transferrin (TF, P589S, hemochromatosis (HFE C282Y and H63D], iron biochemical variables [iron, Tf, ceruloplasmin (Cp, Cp:Tf ratio and % of Tf saturation (% Tf-sat] Impairment (MCI, 78 Parkinson’s disease (PD patients and 139 healthy controls to investigate mechanisms of iron regulation or toxicity. No difference in genetic variant distributions between patients and controls was found in our Italian sample, but the stratification for the APOE e4 allele revealed that among the APOE e4 carriers was higher the frequency of those carriers of at least a mutated TF P589S allele. Decreased Tf in both AD and MCI and increased Cp:Tf ratio in AD vs. controls were detected. A multinomial logistic regression model revealed that increased iron and Cp:Tf ratio and being man instead of woman increased the risk of having PD, that increased values of Cp:Tf ratio corresponded to a 4-fold increase of the relative risk of having MCI, while higher Cp levels were protective for PD and MCI. Our study has some limitations: the small size of the sample, one ethnic group considered, the rarity of some alleles which prevent the statistical power of some genetic analysis. Even though they need confirmation in larger cohorts, our data suggest the hypothesis that deregulation of iron metabolism, in addition to other factors, has some effect on the PD disease risk.

  19. CT findings of Wilson's disease

    International Nuclear Information System (INIS)

    Thirteen cases of Wilson's disease were examined by computerized tomography. Two of them were latent cases. The other 11 were typical cases with a Kayser-Fleisher ring and neurological signs, and in which the ceruloplasmin level in serum was low. The caudate heads were measured by Barr's method using two ratios, FH/CC and CC/OT. The CT findings were as follows: (1) caudate head atrophy (10 cases), (2) cerebral atrophy and/or ventricular dilatation (7 cases), (3) symmetrical low density of thalamus (3 cases), (4) symmetrical low density of pallidum (2 cases), (5) low density of midbrain (2 cases), (6) symmetrical low density of putamen (1 case), (7) pons atrophy (1 case), (8) cerebellar atrophy (1 case), (9) low density of r-temporal area (1 case). All of them except for the two latent cases showed some abnormal findings on CT. Only one symptomatic case showed no caudate atrophy one year after the onset, though two other cases already showed marked atrophy after only 10 months. It was stressed that the low-density lesions in the thalamic area were found with a high frequency. There was no correlation between the duration of illness and the degree of caudate atrophy among the patients with Wilson's disease as compared with those with Huntington's chorea. As in a previous study of pneumoencephalography, we failed also to distinguish the two diseases by measuring the ratios on CT films. It may be valuable to study the progression of the CNS lesions of Wilson's disease by using CT repeatedly. (author)

  20. Acute phase protein response in cows with staphylococcus aureus subclinical mastitis

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    Kovačević-Filipović Milica

    2010-01-01

    Full Text Available Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30 were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group, second group were cows with one quarter infected with Staph. aureus (SaQ1 and third group were cows with two quarters infected (SaQ2. Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA and somatic cell count (SCC. Serum samples where analyzed for SAA, haptoglobin (Hp, ceruloplasmin (Cp and albumin concentration. Also, complete blood count (CBC was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved.

  1. Identification of genomic biomarkers for concurrent diagnosis of drug-induced renal tubular injury using a large-scale toxicogenomics database

    International Nuclear Information System (INIS)

    Drug-induced renal tubular injury is one of the major concerns in preclinical safety evaluations. Toxicogenomics is becoming a generally accepted approach for identifying chemicals with potential safety problems. In the present study, we analyzed 33 nephrotoxicants and 8 non-nephrotoxic hepatotoxicants to elucidate time- and dose-dependent global gene expression changes associated with proximal tubular toxicity. The compounds were administered orally or intravenously once daily to male Sprague-Dawley rats. The animals were exposed to four different doses of the compounds, and kidney tissues were collected on days 4, 8, 15, and 29. Gene expression profiles were generated from kidney RNA by using Affymetrix GeneChips and analyzed in conjunction with the histopathological changes. We used the filter-type gene selection algorithm based on t-statistics conjugated with the SVM classifier, and achieved a sensitivity of 90% with a selectivity of 90%. Then, 92 genes were extracted as the genomic biomarker candidates that were used to construct the classifier. The gene list contains well-known biomarkers, such as Kidney injury molecule 1, Ceruloplasmin, Clusterin, Tissue inhibitor of metallopeptidase 1, and also novel biomarker candidates. Most of the genes involved in tissue remodeling, the immune/inflammatory response, cell adhesion/proliferation/migration, and metabolism were predominantly up-regulated. Down-regulated genes participated in cell adhesion/proliferation/migration, membrane transport, and signal transduction. Our classifier has better prediction accuracy than any of the well-known biomarkers. Therefore, the toxicogenomics approach would be useful for concurrent diagnosis of renal tubular injury.

  2. INFLUENCE OF ACUTE EXERCISE ON OXIDATIVE STRESS IN CHRONIC SMOKERS

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    Zehra Serdar

    2003-09-01

    Full Text Available The relative oxidative insult caused by exercise and smoking on biological systems are well documented, however, their cumulative influence needs to be clarified. In order to examine the collective effects of exercise and smoking on oxidant and antioxidant parameters, young male smokers (n=10 and non-smokers (n=10 made to perform a negative slope (10% cycling exercise for 30 minutes at individual load equivalent to 60% maximal oxygen consumption (VO2max. Pre- and post-exercise (post-ex haematocrit, haemoglobin, white blood cells, plasma malondialdehyde (MDA levels, protein carbonyl formation and non-HDL oxidation, erythrocyte superoxide dismutase (SOD and glutathione peroxidase (GPX activities, serum ceruloplasmin (CER and urinary cotinine concentrations were evaluated. Pre-ex CER and urinary cotinine concentrations of smokers were significantly higher (p<0.05 and p<0.01, respectively compared to that of non-smokers and pre-ex CER concentrations were significantly correlated with cotinine levels in all subjects (p<0.05. Significant (p<0.01 increases were observed in non-HDL oxidation following the exercise in both groups and the elevations were more pronounced in smokers. Pre-ex SOD and GPX activities were not different between the two groups, however post-ex enzyme activities were significantly reduced in smokers (p<0.05. MDA and protein carbonyl concentrations were not different between the two groups and there were not any significant changes due to exercise.In conclusion, according to the results of the present study, we suggest that erythrocyte antioxidants SOD and GPX and plasma non-HDL are more prone to the possible oxidant damage of acute physical exercise in chronic smokers.

  3. High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats.

    Science.gov (United States)

    Ha, Jung-Heun; Doguer, Caglar; Wang, Xiaoyu; Flores, Shireen R; Collins, James F

    2016-01-01

    Iron-copper interactions were described decades ago; however, molecular mechanisms linking the two essential minerals remain largely undefined. Investigations in humans and other mammals noted that copper levels increase in the intestinal mucosa, liver and blood during iron deficiency, tissues all important for iron homeostasis. The current study was undertaken to test the hypothesis that dietary copper influences iron homeostasis during iron deficiency and iron overload. We thus fed weanling, male Sprague-Dawley rats (n = 6-11/group) AIN-93G-based diets containing high (~8800 ppm), adequate (~80) or low (~11) iron in combination with high (~183), adequate (~8) or low (~0.9) copper for 5 weeks. Subsequently, the iron- and copper-related phenotype of the rats was assessed. Rats fed the low-iron diets grew slower than controls, with changes in dietary copper not further influencing growth. Unexpectedly, however, high-iron (HFe) feeding also impaired growth. Furthermore, consumption of the HFe diet caused cardiac hypertrophy, anemia, low serum and tissue copper levels and decreased circulating ceruloplasmin activity. Intriguingly, these physiologic perturbations were prevented by adding extra copper to the HFe diet. Furthermore, higher copper levels in the HFe diet increased serum nonheme iron concentration and transferrin saturation, exacerbated hepatic nonheme iron loading and attenuated splenic nonheme iron accumulation. Moreover, serum erythropoietin levels, and splenic erythroferrone and hepatic hepcidin mRNA levels were altered by the dietary treatments in unanticipated ways, providing insight into how iron and copper influence expression of these hormones. We conclude that high-iron feeding of weanling rats causes systemic copper deficiency, and further, that copper influences the iron-overload phenotype. PMID:27537180

  4. Hypothalamic digoxin, hemispheric chemical dominance, and oncogenesis: evidence from multiple myeloma.

    Science.gov (United States)

    Kurup, Ravi Kumar; Kurup, Paramesware Achutha

    2003-12-01

    This study assessed the changes in the isoprenoid pathway and its metabolites digoxin, dolichol, and ubiquinone in multiple myeloma. The isoprenoid pathway and digoxin status were also studied for comparison in individuals of differing hemispheric dominance to find out the rote of cerebral dominance in the genesis of multiple myeloma and neoplasms. The following parameters were assessed: isoprenoid pathway metabolites, tyrosine and tryptophan catabolites, glycoconjugate metabolism, RBC membrane composition, and free radical metabolism--in multiple myeloma, as well as in individuals of differing hemispheric dominance. There was elevation in plasma HMG CoA reductase activity, serum digoxin, and dolichol, and a reduction in RBC membrane Na(+)-K+ ATPase activity, serum ubiquinone, and magnesium levels. Serum tryptophan, serotonin, nicotine, strychnine, and quinolinic acid were elevated, while tyrosine, dopamine, noradrenaline, and morphine were decreased. The total serum glycosaminoglycans and glycosaminoglycan fractions, the activity of GAG degrading enzymes and glycohydrolases, carbohydrate residues of glycoproteins, and serum glycolipids were elevated. The RBC membrane glycosaminoglycans, hexose, and fucose residues of glycoproteins, cholesterol, and phospholipids were reduced. The activity of all free-radical scavenging enzymes, concentration of glutathione, iron binding capacity, and ceruloplasmin decreased significantly, while the concentration of lipid peroxidation products and nitric oxide increased. Hyperdigoxinemia-related altered intracellular Ca++/Mg++ ratios mediated oncogene activation, dolichol-induced altered glycoconjugate metabolism, and ubiquinone deficiency-related mitochondrial dysfunction can contribute to the pathogenesis of multiple myeloma. The biochemical patterns obtained in multiple myeloma are similar to those obtained in left-handed/right hemispheric chemically dominant individuals by the dichotic listening test. But all the patients with

  5. Correlation between the serum and tissue levels of oxidative stress markers and the extent of inflammation in acute appendicitis

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    Ersin Gürkan Dumlu

    2014-12-01

    Full Text Available OBJECTIVES: To determine the serum and tissue levels of markers of impaired oxidative metabolism and correlate these levels with the histopathology and Alvarado score of acute appendicitis patients. METHOD: Sixty-five acute appendicitis patients (mean age, 31.4±12.06 years; male/female, 30/35 and 30 healthy control subjects were studied. The Alvarado score was recorded. Serum samples were obtained before surgery and 12 hours postoperatively to examine the total antioxidant status, total oxidant status, paraoxonase, stimulated paraoxonase, arylesterase, catalase, myeloperoxidase, ceruloplasmin, oxidative stress markers (advanced oxidized protein products and total thiol level and ischemia-modified albumin. Surgical specimens were also evaluated. RESULTS: The diagnoses were acute appendicitis (n = 37, perforated appendicitis (n = 8, phlegmonous appendicitis (n = 12, perforated+phlegmonous appendicitis (n = 4, or no appendicitis (n = 4. The Alvarado score of the acute appendicitis group was significantly lower than that of the perforated+phlegmonous appendicitis group (p = 0.004. The serum total antioxidant status, total thiol level, advanced oxidized protein products, total oxidant status, catalase, arylesterase, and ischemia-modified albumin levels were significantly different between the acute appendicitis and control groups. There was no correlation between the pathological extent of acute appendicitis and the tissue levels of the markers; additionally, there was no correlation between the tissue and serum levels of any of the parameters. CONCLUSIONS: The imbalance of oxidant/antioxidant systems plays a role in the pathogenesis acute appendicitis. The Alvarado score can successfully predict the presence and extent of acute appendicitis.

  6. Menkes disease – An important cause of early onset refractory seizures

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    Puneet Jain

    2014-01-01

    Full Text Available Context: Menkes disease is an X-linked multisystem disorder characterized by early onset of cerebral and cerebellar neurodegeneration, fair skin, hypopigmented sparse hair and connective tissue abnormalities. Aims: We aimed to evaluate the clinical, electrophysiological and radiological features of children with Menkes disease seen at our institute. Setting/Design: The medical records of children diagnosed with Menkes disease admitted in the pediatric neurology ward or attending the special pediatric neurology clinic at a tertiary care and a referral hospital in North India, from January 2010 to December 2012, were retrospectively reviewed. The clinical data of each case was subsequently summarized and reported. Statistical analysis used: Descriptive statistics were used. Results: During the study period, 1174 children were seen. Out of these, 6 cases were diagnosed as Menkes disease on the basis of clinical phenotype, low serum copper and ceruloplasmin and supportive neuroimaging. All the children were males and had disease onset within 3 months of age, with 4 children presenting in the neonatal period. Global developmental delay and refractory seizures were the predominant clinical symptoms. Two children had symptomatic West syndrome. Other seizure semiologies included tonic-clonic (4, myoclonic (2 and tonic seizures (1. The electroencephalographic abnormalities included hypsarrythmia (2 and multifocal epileptiform discharges (3. The salient radiological features included white matter changes, temporal lobe abnormalities, global atrophy, subdural hygromas and tortuous cerebral blood vessels. Conclusions: Menkes disease should be suspected in a case of refractory early onset seizures especially in the presence of subtle clinical clues. The neuroimaging findings may further support the diagnosis.

  7. Experimental Investigation of the Ferrum (III Hydroxide Polymaltosate Preparation Influence Peculiarities on the Lipid Peroxidation Values

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    Lovtsova L.V.

    2010-09-01

    Full Text Available Aim of investigation is a study of the ferrum (III hydroxide polymaltosate preparation influence peculiarities on the lipid peroxidation and antioxidant protection values in blood of experimental animals at the background of a ferrodeficient anemia. Materials and Methods. The experiment is made on 120 nonlinear male-rats. The values of induced biochemiluminescence, a content of malone dialdehyde, an activity of superoxidedismutase and catalase, a concentration of ceruloplasmin in blood of experimental animals at infusion of a preparation, containing a trivalent ferrum, — a ferrum (III hydroxide polymaltosate (maltofer — in equitherapeutic dose of 17.14 mg/kg a day (calculating the elementary ferrum have been detected during investigation for 30 days. Results. A trivalent ferrum (III hydroxide polymaltosate preparation (maltofer stipulates at the early stages an insignificant, and at the later stages — expressed influence on the pointed process, which is confirmed by an increase of a free-radical oxidation process abatement rate in blood plasma of experimental animals, and also a decrease of a superoxidedismutase activity (in a day of its infusion, a decrease of a malone dialdehyde concentration (in 5 days, a decrease of the antioxidant and superoxidedismutase activity (in 10 days, an increase of a plasma free-radical activity and a free-radical oxidation process abatement rate (in 20 days, a decrease of a malone dialdehyde content (in 30 days. Conclusion. The investigating trivalent ferrum preparation activates a process of lipid peroxidation. Its distinctive feature is a more expressed influence on the pointed process at the late stages of its infusion.

  8. Creutzfeldt-Jakob disease, Heidenhain variant: case report with MRI (DWI) findings

    International Nuclear Information System (INIS)

    Creutzfeldt-Jakob disease (CJD) is a pre senile dementia characterized by rapidly progressive mental deterioration, myoclonic jerking, and other less common neurological signs. Few accentuates cases have been described in Brazil. A 54-year-old white woman, was admitted in our service with a month history of progressive, bilateral cortical blindness. After admission, she developed right partial motor seizures (right facial, upper and lower limbs), she became progressively aphasic (mixed aphasia). Seizures were controlled with phenytoine, but she developed choreoathetotic movements on her right dimidium, with partial control after introduction of chlorpromazine 25 mg q/d. She could no longer stand up or walk due to severe ataxia. The first EEG (October, 2001) showed left hemisphere severe seizure activity (status epilepticus partial is). She was delivered home with enteral nutrition, phenytoine, chlorpromazine and mepacrine 100 mg q d. The following laboratory tests were negative or normal: blood series, platelets, ESR, kidney and liver function, copper, ceruloplasmin, Vedril, HIV, HTLV-1, lactate, and cerebral Dsa (performed in other service). A spinal tap with normal opening pressure was perform and CSFR examination was normal. CSFR 14-3-3 protein was positive, CSF specific neuronal enolase 7.5 ng/ml(normal). Genetic study of PRNP gene did not disclosed any known mutation. A MRI (October, 2001) showed areas of hyperintense signal (T 2 and FLAIR) without Gd-enhancement on T1, in the left temporal lobe and in both occipital lobes; basal ganglia have a normal appearance. DWI imaging showed bright areas at the same sites. An EEG (March, 2002) disclosed a periodical sharp triphasic waves pattern, suggestive of CJD. A second MRI (April, 2002) showed mild generalized atrophy, no ventricular dilatation, and the hyperintense sites disappeared. She remained clinically stable and under use of chlorpromazine and mepacrine until she died due to pulmonary complications on April

  9. A Review and Current Perspective on Wilson Disease

    Science.gov (United States)

    Patil, Mallikarjun; Sheth, Keyur A.; Krishnamurthy, Adarsh C.; Devarbhavi, Harshad

    2013-01-01

    Wilson disease is a rare, inherited autosomal recessive disease of copper metabolism and may be more common where consanguinity is prevalent. Much has been known about the disease after it was first described by Kinnier Wilson as ‘progressive lenticular degeneration in 1912. Over 500 mutations of the ATP7B gene has been identified with no clear genotype to phenotype correlation. Loss of ATP7B function leads various grades of reduced biliary excretion of copper and reduced incorporation of copper into ceruloplasmin; accumulation and toxicity of copper in the liver, brain and other tissues results in liver toxicity and other myriad manifestations of the disease. The clinical features may vary from asymptomatic state to chronic liver disease, acute liver failure, neuropsychiatric manifestations and hemolytic anemia. Diagnosis is based on the combination of clinical sign's, biochemical features, histologic findings and mutation analysis of ATP7B gene. Subtle geographical differences exist with a disproportionate proportion of children presenting with acute liver failure. A high index of suspicion is needed for an early diagnosis. Ratios of biochemical indices for early diagnosis need validation across geographical regions and may not be particularly applicable in children. Better biomarkers or the need for tests for early detection of ALF persists. Drugs used in the treatment of Wilson disease include copper chelating agents such as d-Penicillamine, trientine and zinc salt. Untreated Wilson disease uniformly leads to death from liver disease or severe neurological disability. Early recognition and treatment has excellent prognosis. Liver transplantation is indicated in acute liver failure and end stage liver disease. Family screening in order to detect the disorder in the first-degree relatives is warranted. This review provides an overview of different aspects of Wilson disease including geographical differences in presentations and clinical management and the

  10. Urinary proteomic shotgun approach for identification of potential acute rejection biomarkers in renal transplant recipients

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    Loftheim Håvard

    2012-08-01

    Full Text Available Abstract Background Acute rejection (AR episodes in renal transplant recipients are suspected when plasma creatinine is elevated and other potential causes out ruled. Graft biopsies are however needed for definite diagnosis. Non-invasive AR-biomarkers is an unmet clinical need. The urinary proteome is an interesting source in the search for such a biomarker in this population. Methods In this proof of principle study, serial urine samples in the early post transplant phase from 6 patients with biopsy verified acute rejections and 6 age-matched controls without clinical signs of rejection were analyzed by shotgun proteomics. Results Eleven proteins fulfilled predefined criteria for regulation in association with AR. They presented detectable regulation already several days before clinical suspicion of AR (increased plasma creatinine. The regulated proteins could be grouped by their biological function; proteins related to growth and proteins related to immune response. Growth-related proteins (IGFBP7, Vasorin, EGF and Galectin-3-binding protein were significantly up-regulated in association with AR (P = 0.03 while proteins related to immune response (MASP2, C3, CD59, Ceruloplasmin, PiGR and CD74 tended to be up-regulated ( P = 0.13. Conclusion The use of shotgun proteomics provides a robust and sensitive method for identification of potentially predictive urinary biomarkers of AR. Further validation of the current findings is needed to establish their potential clinical role with regards to clinical AR diagnosis. Trial registration ClinicalTrials.gov number NCT00139009

  11. Subacute sclerosing leucoencephalitis: an epidemiological, clinical and biochemical study of 31 cases

    Directory of Open Access Journals (Sweden)

    Horácio M. Canelas

    1967-12-01

    Full Text Available Thirty-one cases of SSLE are reported. The diagnosis was based on the clinical picture, the marked increase of gammaglobulins in the cerebrospinal fluid, the typical electroencephalographic pattern, and the pathological examination. Intracellular inclusion bodies were found at the postmortem examination of 8 among 13 cases and in 1 of 7 cerebral biopsies. The mean age was 11.7 years, with the limits of 3 and 22 years. Males prevailed markedly over females (23:8. Patients from rural regions were the bulk of the series (77.4%. No definite regional predominance was demonstrated by the analysis of the geographic distribution. A seasonal influence was not evidenced. In 16 patients followed up to death, the average duration of the disease was 7 months, with a range from 2 to 20 months. In the clinical picture the constancy of myoclonus or losses of tonus, and psychiatric disorders was evidenced. The first symptom was the psychiatric alteration in 55.6% of the cases, myoclonus in 35.5% and grand mal seizures in 33.3%. Rigidity and pyramidal signs were very frequent, while the cerebellar syndrome was rare. Ophtalmoscopic alterations (edematous or pale disk, choroidits were found in a third of the cases. In two cases an epizootic of household chicken was coincident with the onset of the disease. The blood copper concentrations determined in 8 cases were always above the normal range, while ceruloplasmin remained within the normal limits. The blood sulfur levels were high in 3 patients studied. The blood magnesium levels were variable in 6 patients studied.

  12. Varying Dietary Levels of Molybdenum Inducing Cell Apoptosis of Spleen Under Cadmium Stress in Caprine.

    Science.gov (United States)

    Xiao, Qingyang; Zhang, Caiying; Gu, Xiaolong; Zhuang, Yu; Luo, Junrong; Liu, Ping; Guo, Xiaoquan; Hu, Guoliang; Cao, Huabin

    2016-07-01

    The present experiment aims at evaluating chronic toxic effects of the combination of cadmium (Cd) and molybdenum (Mo) according to residual element contents, apoptosis gene expression, and ultrastructure and histopathology changes of caprine spleen. In total, 36 Boer goats were randomly divided into four groups with the equal number in each group. The control group was orally administered with deionized water while the experimental groups I, II, and III were administered with the equal quantity of CdCl2 (1 mg kg(-1) BW) and (NH4)6·Mo7O24·4H2O including 15, 30, and 45 mg·Mo kg(-1) BW, respectively. Three individuals from each group were treated with euthanasia on days 0, 25, and 50. The data showed that the content of splenic residual Mo and Cd increased (P < 0.05) in the experimental groups on days 25 and 50, while no significant difference was observed in the content of Cu. The apoptosis-related gene expression levels including Bcl-2, Bax, Caspase-3, Smac, and ceruloplasmin (CP) were also determined. Results showed that significant reductions were observed in Bcl-2 and CP expressions (P < 0.01), while Caspase-3 gene was up-regulated (P < 0.05). However, no significant difference was observed in Smac and Bax expressions. Furthermore, on day 50, spleen tissues were presented to observe ultrastructural changes in lesions by means of transmission electron microscopy, with fragmentized nucleus, vesiculation of cytoplasm, mitochondria hyperplasia, and increasing lysosomes included. In addition, histopathology results corroborated the toxicity by showing cell hemorrhage, thickening central arteries, and enhanced capsule thickness. To sum up, our study revealed that the combination of Cd and Mo could induce remarkable damage to the spleen of goats by promoting cell apoptosis in the mitochondrial pathway and affecting the deposition of Mo and Cd. PMID:26585322

  13. Creutzfeldt-Jakob disease, Heidenhain variant: case report with MRI (DWI) findings; Doenca de Creutzfeldt-Jakob forma Heidenhain: relato de caso com achados de ressonancia magnetica e DWI

    Energy Technology Data Exchange (ETDEWEB)

    Arruda, Walter Oleschko; Bordignon, Kelly C.; Milano, Jeronimo B.; Ramina, Ricardo [Instituto de Neurologia de Curitiba, PR (Brazil)]. E-mail: warruda@speednet.com.br

    2004-06-01

    Creutzfeldt-Jakob disease (CJD) is a pre senile dementia characterized by rapidly progressive mental deterioration, myoclonic jerking, and other less common neurological signs. Few accentuates cases have been described in Brazil. A 54-year-old white woman, was admitted in our service with a month history of progressive, bilateral cortical blindness. After admission, she developed right partial motor seizures (right facial, upper and lower limbs), she became progressively aphasic (mixed aphasia). Seizures were controlled with phenytoine, but she developed choreoathetotic movements on her right dimidium, with partial control after introduction of chlorpromazine 25 mg q/d. She could no longer stand up or walk due to severe ataxia. The first EEG (October, 2001) showed left hemisphere severe seizure activity (status epilepticus partial is). She was delivered home with enteral nutrition, phenytoine, chlorpromazine and mepacrine 100 mg q d. The following laboratory tests were negative or normal: blood series, platelets, ESR, kidney and liver function, copper, ceruloplasmin, Vedril, HIV, HTLV-1, lactate, and cerebral Dsa (performed in other service). A spinal tap with normal opening pressure was perform and CSFR examination was normal. CSFR 14-3-3 protein was positive, CSF specific neuronal enolase 7.5 ng/ml(normal). Genetic study of PRNP gene did not disclosed any known mutation. A MRI (October, 2001) showed areas of hyperintense signal (T 2 and FLAIR) without Gd-enhancement on T1, in the left temporal lobe and in both occipital lobes; basal ganglia have a normal appearance. DWI imaging showed bright areas at the same sites. An EEG (March, 2002) disclosed a periodical sharp triphasic waves pattern, suggestive of CJD. A second MRI (April, 2002) showed mild generalized atrophy, no ventricular dilatation, and the hyperintense sites disappeared. She remained clinically stable and under use of chlorpromazine and mepacrine until she died due to pulmonary complications on April

  14. Proteomic Analysis of Serum from Patients with Major Depressive Disorder to Compare Their Depressive and Remission Statuses

    Science.gov (United States)

    Lee, Jiyeong; Joo, Eun-Jeong; Lim, Hee-Joung; Park, Jong-Moon; Lee, Kyu Young; Park, Arum; Seok, AeEun

    2015-01-01

    Objective Currently, there are a few biological markers to aid in the diagnosis and treatment of depression. However, it is not sufficient for diagnosis. We attempted to identify differentially expressed proteins during depressive moods as putative diagnostic biomarkers by using quantitative proteomic analysis of serum. Methods Blood samples were collected twice from five patients with major depressive disorder (MDD) at depressive status before treatment and at remission status during treatment. Samples were individually analyzed by liquid chromatography-tandem mass spectrometry for protein profiling. Differentially expressed proteins were analyzed by label-free quantification. Enzyme-linked immunosorbent assay (ELISA) results and receiver-operating characteristic (ROC) curves were used to validate the differentially expressed proteins. For validation, 8 patients with MDD including 3 additional patients and 8 matched normal controls were analyzed. Results The quantitative proteomic studies identified 10 proteins that were consistently upregulated or downregulated in 5 MDD patients. ELISA yielded results consistent with the proteomic analysis for 3 proteins. Expression levels were significantly different between normal controls and MDD patients. The 3 proteins were ceruloplasmin, inter-alpha-trypsin inhibitor heavy chain H4 and complement component 1qC, which were upregulated during the depressive status. The depressive status could be distinguished from the euthymic status from the ROC curves for these proteins, and this discrimination was enhanced when all 3 proteins were analyzed together. Conclusion This is the first proteomic study in MDD patients to compare intra-individual differences dependent on mood. This technique could be a useful approach to identify MDD biomarkers, but requires additional proteomic studies for validation. PMID:25866527

  15. The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish (Carassius auratus L.).

    Science.gov (United States)

    Kovacevic, Nikolina; Hagen, Mariel O; Xie, Jiasong; Belosevic, Miodrag

    2015-11-01

    The expression of genes encoding the acute phase proteins (APP) during the course of Trypanasoma carassii infection in the goldfish was determined using quantitative PCR. Significant changes in the mRNA levels of ceruloplasmin (Cp), C-reactive protein (CRP), transferrin (Tf), hemopexin (Hx) and serum amyloid A (SAA) were observed in the kidney, liver and spleen at various days post infection (dpi). Of the five acute phase protein genes examined, CRP and SAA exhibited the highest expression in the tissues during the acute infection. Cp and Tf were up-regulated throughout the acute course of infection in the liver. During the chronic phase of the infection, APP expression in the liver was similar to that in the non-infected control fish. At 7 dpi, Cp, Tf and Hx were down-regulated in the spleen, and Cp and Tf kidney, but their mRNA levels gradually returned to those of control non-infected fish. In contrast, during the chronic phase of the infection, there was an up-regulation of Cp, Hx and Tf in the spleen, and Tf and SAA in the kidney. The goldfish CRP was cloned and functionally characterized. CRP was differentially expressed in normal goldfish immune cells, with highest expression in monocytes and lowest expression in mature macrophages. A recombinant goldfish CRP (rgfCRP) was generated using prokaryotic expression. rgfCRP enhanced complement-mediated killing of trypanosomes in vitro, and the lysis increased after addition of immune serum. rgfCRP did not affect the production of reactive oxygen and nitrogen intermediates by monocytes and macrophages, respectively. PMID:26116443

  16. Immunometabolic Status during the Peripartum Period Is Enhanced with Supplemental Zn, Mn, and Cu from Amino Acid Complexes and Co from Co Glucoheptonate.

    Directory of Open Access Journals (Sweden)

    Fernanda Batistel

    Full Text Available The peripartum (or transition period is the most-critical phase in the productive life of lactating dairy cows and optimal supply of trace minerals through more bioavailable forms could minimize the negative effects associated with this phase. Twenty Holstein cows received a common prepartal diet and postpartal diet. Both diets were partially supplemented with an inorganic (INO mix of Zn, Mn, and Cu to supply 35, 45, and 6 ppm, respectively, of the diet dry matter (DM. Cows were assigned to treatments in a randomized completed block design, receiving an daily oral bolus with INO or organic trace minerals (AAC Zn, Mn, Cu, and Co to achieve 75, 65, 11, and 1 ppm supplemental, respectively, in the diet DM. Liver tissue and blood samples were collected throughout the experiment. The lower glutamic-oxaloacetic transaminase concentration after 15 days in milk in AAC cows indicate lower hepatic cell damage. The concentration of cholesterol and albumin increased, while IL-6 decreased over time in AAC cows compared with INO indicating a lower degree of inflammation and better liver function. Although the acute-phase protein ceruloplasmin tended to be lower in AAC cows and corresponded with the reduction in the inflammatory status, the tendency for greater serum amyloid A concentration in AAC indicated an inconsistent response on acute-phase proteins. Oxygen radical absorbance capacity increased over time in AAC cows. Furthermore, the concentrations of nitric oxide, nitrite, nitrate, and the ferric reducing ability of plasma decreased with AAC indicating a lower oxidative stress status. The expression of IL10 and ALB in liver tissue was greater overall in AAC cows reinforcing the anti-inflammatory response detected in plasma. The greater overall expression of PCK1 in AAC cows indicated a greater gluconeogenic capacity, and partly explained the greater milk production response over time. Overall, feeding organic trace minerals as complexed with amino acids

  17. Immunometabolic Status during the Peripartum Period Is Enhanced with Supplemental Zn, Mn, and Cu from Amino Acid Complexes and Co from Co Glucoheptonate.

    Science.gov (United States)

    Batistel, Fernanda; Osorio, Johan S; Ferrari, Annarita; Trevisi, Erminio; Socha, Michael T; Loor, Juan J

    2016-01-01

    The peripartum (or transition) period is the most-critical phase in the productive life of lactating dairy cows and optimal supply of trace minerals through more bioavailable forms could minimize the negative effects associated with this phase. Twenty Holstein cows received a common prepartal diet and postpartal diet. Both diets were partially supplemented with an inorganic (INO) mix of Zn, Mn, and Cu to supply 35, 45, and 6 ppm, respectively, of the diet dry matter (DM). Cows were assigned to treatments in a randomized completed block design, receiving an daily oral bolus with INO or organic trace minerals (AAC) Zn, Mn, Cu, and Co to achieve 75, 65, 11, and 1 ppm supplemental, respectively, in the diet DM. Liver tissue and blood samples were collected throughout the experiment. The lower glutamic-oxaloacetic transaminase concentration after 15 days in milk in AAC cows indicate lower hepatic cell damage. The concentration of cholesterol and albumin increased, while IL-6 decreased over time in AAC cows compared with INO indicating a lower degree of inflammation and better liver function. Although the acute-phase protein ceruloplasmin tended to be lower in AAC cows and corresponded with the reduction in the inflammatory status, the tendency for greater serum amyloid A concentration in AAC indicated an inconsistent response on acute-phase proteins. Oxygen radical absorbance capacity increased over time in AAC cows. Furthermore, the concentrations of nitric oxide, nitrite, nitrate, and the ferric reducing ability of plasma decreased with AAC indicating a lower oxidative stress status. The expression of IL10 and ALB in liver tissue was greater overall in AAC cows reinforcing the anti-inflammatory response detected in plasma. The greater overall expression of PCK1 in AAC cows indicated a greater gluconeogenic capacity, and partly explained the greater milk production response over time. Overall, feeding organic trace minerals as complexed with amino acids during the

  18. Interactions between copper deficiency, selenium deficiency and adriamycin toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, J.; Tackett, R.; Johnson, M.A. (Univ. of Georgia, Athens (United States))

    1991-03-15

    The objective of this study was to test the hypothesis that there are interactions between copper (Cu) and selenium (Se) status, and adriamycin (ADR) toxicity. Male Sprague Dawley rats were fed Cu,Se adequate; Cu deficient, Se adequate ({minus}Cu); Cu adequate, Se deficient; or Cu,Se deficient diets for 38-41 days. ADR or saline (SAL) were administered weekly for the last 4 weeks of the study. Cu deficiency was confirmed by a 3-fold decrease in liver Cu,Zn-superoxide dismutase and liver Cu, and a 5-fold decrease in RBC Cu,Zn-SOD. Se deficiency was confirmed by a 10-fold decrease in liver glutathione peroxidase (GSH-Px). ADR, Cu deficiency and Se deficiency all caused EKG abnormalities. However, Cu and Se deficiencies did not enhance ADR's influence on EKGs. ADR increased lipid peroxidation in liver by 15% and in heart by 18% (NS). Cu deficiency decreased ADR-induced lipid peroxidation in heart tissue by 25%. ADR influenced Se status by significantly increasing heart GSH-Px, and Cu status by increasing liver Cu, plasma ceruloplasmin and liver Cu, Zn-SOD. These elevations in Cu,Zn-SOD and GSH-Px may be a consequence of the increased lipid peroxidation initiated by ADR. In {minus}Cu rats, ADR caused severe hemolytic anemia characterized by a 19% decrease in hematocrit and a 17-fold increase in splenic Fe. These data suggest that there are numerous interactions between ADR toxicity and Cu and Se status.

  19. Evaluation of antiinflammatory activity ofTephrosia purpurea in rats

    Institute of Scientific and Technical Information of China (English)

    Shenoy Smita; Shwetha K; Prabhu K; Maradi R; Bairy KL; Shanbhag T

    2010-01-01

    Objective:To evaluate the antiinflammatory activity of orally administered ethanolic extract of Tephrosia purpurea in acute and subacute inflammation in rats.Methods: An ethanolic extract of Tephrosia purpurea was prepared. Carrageenan induced paw edema and cotton pellet granuloma were the models for acute and subacute inflammation respectively. Four groups of rats in each model were treated orally with 2% gum acacia, 100 mg /kg of aspirin, 500 mg/kg and 1 000 mg/kg of ethanolic extract ofTephrosia purpurea respectively. In carrageenan induced paw edema model, subplantar injection of 1% carrageenan was made into the hind paw of the rats sixty minutes after the administration of the respective drugs. The paw volume was measured immediately after injection of carrageenan, at 3 hours and at 6 hours. Then percentage inhibition of edema was calculated. In the cotton pellet granuloma model, animals were administered drugs for six days after placing cotton pellets in the axilla on each side. On the 7th day, dry weight of granuloma was calculated.Results:The rats treated withTephrosia purpurea did not exhibit any significant decrease in paw volume and serum ceruloplasmin levels as compared to the control and aspirin treated groups in the acute inflammation model; while, there was a significant (P < 0.01) decrease in the weight of granuloma inTephrosia purpurea and aspirin treated groups as compared to control in subacute inflammation.Conclusions:The ethanolic extract of orally administered Tephrosia purpurea shows significant antiinflammatory effect in subacute inflammation but not in acute inflammation in rats.

  20. Response to dietary supplementation of L-glutamine and L-glutamate in broiler chickens reared at different stocking densities under hot, humid tropical conditions.

    Science.gov (United States)

    Shakeri, M; Zulkifli, I; Soleimani, A F; O'Reilly, E L; Eckersall, P D; Anna, A A; Kumari, S; Abdullah, F F J

    2014-11-01

    A study was conducted to determine whether supplementing AminoGut (a commercial dietary supplement containing a mixture of l-glutamine and l-glutamic acid) to broiler chickens stocked at 2 different densities affected performance, physiological stress responses, foot pad dermatitis incidence, and intestinal morphology and microflora. A randomized design in a factorial arrangement with 4 diets [basal diet, basal diet + 0.5% AminoGut from d 1 to 21, basal diet + 0.5% AminoGut from d 1 to 42, and basal diet + virginiamycin (0.02%) for d 1 to 42] and 2 stocking densities [0.100 m(2)/bird (23 birds/pen; LD) or 0.067 m(2)/bird (35 birds/pen; HD)]. Results showed that villi length and crypt depth were not changed by different dietary treatments. However, birds in the HD group had smaller villi (P = 0.03) compared with those of the LD group. Regardless of diet, HD consistently increased the serum concentrations of ceruloplasmin, α-1 acid glycoprotein, ovotransferin, and corticosterone (P = 0.0007), and elevated heterophil to lymphocyte ratio (0.0005). Neither AminoGut supplementation nor stocking density affected cecal microflora counts. In conclusion, under the conditions of this study, dietary supplementation of AminoGut, irrespective of stocking density, had no beneficial effect on growth performance, intestinal morphology, and physiological adaptive responses of broiler chickens raised under hot and humid tropical conditions. However, AminoGut supplementation from d 1 to 42 was beneficial in reducing mortality rate. Also, the increased serum concentrations of a wide range of acute phase proteins together with elevated corticosterone and heterophil to lymphocyte ratio suggested that high stocking density induced an acute phase response either indirectly as a result of increased incidence of inflammatory diseases such as foot pad dermatitis or possibly as a direct physiological response to the stress of high stocking density. PMID:25143595

  1. Nutritional Assessment During a 14-d Saturation Dive: the NASA Extreme Environment Mission Operation V Project

    Science.gov (United States)

    Smith, S. M.; Davis-Street, J. E.; Fesperman, J. V.; Smith, M. D.; Rice, B. L.; Zwart, S. R.

    2006-01-01

    Ground-based analogs of spaceflight are an important means of studying physiological and nutritional changes associated with space travel, particularly since exploration missions are anticipated, and flight research opportunities are limited. A clinical nutritional assessment of the NASA Extreme Environment Mission Operation V (NEEMO) crew (4 M, 2 F) was conducted before, during, and after the 14-d saturation dive. Blood and urine samples were collected before (D-12 and D-1), during (MD 7 and MD 12), and after (R + 0 and R + 7) the dive. The foods were typical of the spaceflight food system. A number of physiological changes were reported both during the dive and post dive that are also commonly observed during spaceflight. Serum hemoglobin and hematocrit were decreased (P less than 0.05) post dive. Serum ferritin and ceruloplasmin significantly increased during the dive, while transferring receptors tended to go down during the dive and were significantly decreased by the last day (R + 0). Along with significant hematological changes, there was also evidence for increased oxidative damage and stress during the dive. 8-hydroxydeoxyguanosine was elevated (P less than 0.05) during the dive, while glutathione peroxidase and superoxide disrnutase activities were decreased (P less than 0.05) during the dive. Serum C-reactive protein (CRP) concentration also tended to increase during the dive, suggesting the presence of a stress-induced inflammatory response, Decreased leptin during the dive (P less than 0.05) may also be related to the increased stress. Similar to what is observed during spaceflight, subjects had decreased energy intake and weight loss during the dive. Together, these similarities to spaceflight provide a model to further define the physiological effects of spaceflight and investigate potential countermeasures.

  2. Fe and Cu isotope mass balances in the human body

    Science.gov (United States)

    Balter, V.; Albarede, F.; Jaouen, K.

    2011-12-01

    The ranges of the Fe and Cu isotope compositions in the human body are large, i.e. ~3% and ~2%, respectively. Both isotopic fractionations appear to be mainly controlled by redox conditions. The Fe and Cu isotope compositions of the tissues analyzed so far plot on a mixing hyperbolae between a reduced and an oxidized metals pools. The reduced metals pool is composed by erythrocytes, where Fe is bounded to hemoglobin as Fe(II) and Cu to superoxide-dismutase as Cu(I). The oxidized metals pool is composed by hepatocytes, where Fe and Cu are stored as Fe(III) ferritin and as Cu(II) ceruloplasmine, respectively. The position of each biological component in the δ56Fe-δ65Cu diagram therefore reflects the oxidation state of Fe and Cu of the predominant metal carrier protein and allows to quantify Fe and Cu fluxes between organs using mass balance calculations. For instance, serum and clot Fe and Cu isotope compositions show that current biological models of erythropoiesis violates mass conservation requirements, and suggest hidden Fe and Cu pathways during red blood cells synthesis. The results also show that a coupled Fe-Cu strong gender isotopic effect is observed in various organs. The isotopic difference between men and women is unlikely to be due to differential dietary uptake or endometrium loss, but rather reflects the effect of menstrual losses and a correlative solicitation of hepatic stores. We speculate that thorough studies of the metabolism of stable isotopes in normal conditions is a prerequisite for the understanding of the pathological dysregulations.

  3. Oxidative stress

    Directory of Open Access Journals (Sweden)

    Stevanović Jelka

    2012-01-01

    Full Text Available The unceasing need for oxygen is in contradiction to the fact that it is in fact toxic to mammals. Namely, its monovalent reduction can have as a consequence the production of short-living, chemically very active free radicals and certain non-radical agents (nitrogen-oxide, superoxide-anion-radicals, hydroxyl radicals, peroxyl radicals, singlet oxygen, peroxynitrite, hydrogen peroxide, hypochlorous acid, and others. There is no doubt that they have numerous positive roles, but when their production is stepped up to such an extent that the organism cannot eliminate them with its antioxidants (superoxide-dismutase, glutathione-peroxidase, catalase, transferrin, ceruloplasmin, reduced glutathion, and others, a series of disorders is developed that are jointly called „oxidative stress.“ The reactive oxygen species which characterize oxidative stress are capable of attacking all main classes of biological macromolecules, actually proteins, DNA and RNA molecules, and in particular lipids. The free radicals influence lipid peroxidation in cellular membranes, oxidative damage to DNA and RNA molecules, the development of genetic mutations, fragmentation, and the altered function of various protein molecules. All of this results in the following consequences: disrupted permeability of cellular membranes, disrupted cellular signalization and ion homeostasis, reduced or loss of function of damaged proteins, and similar. That is why the free radicals that are released during oxidative stress are considered pathogenic agents of numerous diseases and ageing. The type of damage that will occur, and when it will take place, depends on the nature of the free radicals, their site of action and their source. [Projekat Ministarstva nauke Republike Srbije, br. 173034, br. 175061 i br. 31085

  4. Interaction of phytic acid and zinc affecting copper bioavailability in rats

    International Nuclear Information System (INIS)

    The objectives of this investigation were to develop a protocol to measure Cu bioavailability using four different indices of Cu status, and to evaluate the effect of phytic acid on Cu bioavailability using these indices in the rat. Ninety-six Sprague-Dawley male weanling rats were fed a Cu-deficient diet for four weeks. The Cu-depleted rats were divided into twelve groups and fed test diets containing 1, 2, 3, and 10 ug Cu and 0, 0.4 and 0.8% phytic acid at each Cu level. After 3 days of Cu repletion, liver copper (LCu), liver superoxide dismutase (LSOD) activity, serum copper (SCu), and serum ceruloplasmin (CP) were measured. There was a significant decrease in SCu and LCu in rats fed 30 ug Zn/g or 230 ug Zn/g compared to animals fed 12 ug Zn/g. Phytic acid increased the level of Cu in the sera and livers of rats fed the diet with 30 ug Zn/g, but those of rats fed 12 or 230 ug Zn/g did not. Dietary phytic acid appeared to reduce the synthesis of two intestinal proteins: a high molecular weight protein and metallothionein. The incorporation of 35S-cysteine into both proteins increased with increasing levels of dietary Zn. The results of these studied indicated: (1) LCu and SCu are reliable indices of Cu for absorption and utilization in the rat, and (2) phytic acid increases Cu bioavailability by binding dietary Zn and reduces the level of Zn-induced mucosal proteins which allows Cu to be more efficiently absorbed

  5. Evolution of exchangeable copper and relative exchangeable copper through the course of Wilson's disease in the Long Evans Cinnamon rat.

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    Françoise Schmitt

    Full Text Available BACKGROUND: Wilson's disease (WD is an inherited disorder of copper metabolism leading to liver failure and/or neurological impairment. Its diagnosis often remains difficult even with genetic testing. Relative exchangeable copper (REC has recently been described as a reliable serum diagnostic marker for WD. METHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to validate the use of REC in the Long Evans Cinnamon (LEC rat, an animal model for WD, and to study its relevance under different conditions in comparison with conventional markers. Two groups of LEC rats and one group of Long-Evans (LE rats were clinically and biologically monitored from 6 to 28 weeks of age. One group of LEC rats was given copper-free food. The other groups had normal food. Blood samples were collected each month and different serum markers for WD (namely ceruloplasmin oxidase activity, exchangeable copper (CuEXC, total serum copper and REC and acute liver failure (serum transaminases and bilirubinemia were tested. Every LEC rat under normal food developed acute liver failure (ALF, with 40% global mortality. Serum transaminases and bilirubinemia along with total serum copper and exchangeable copper levels increased with the onset of acute liver failure. A correlation was observed between CuEXC values and the severity of ALF. Cut-off values were different between young and adult rats and evolved because of age and/or liver failure. Only REC, with values >19%, was able to discriminate LEC groups from the LE control group at every time point in the study. REC sensitivity and specificity reached 100% in adults rats. CONCLUSIONS/SIGNIFICANCE: REC appears to be independent of demographic or clinical data in LEC rats. It is a very simple and reliable blood test for the diagnosis of copper toxicosis owing to a lack of ATP7B function. CuEXC can be used as an accurate biomarker of copper overload.

  6. Absorption of dietary manganese by dairy cows and the role of plasma proteins and the liver in its homeostasis

    International Nuclear Information System (INIS)

    The concentration of manganese in the systemic blood plasma of cattle is maintained close to 5 μg/1 whether their diet contains 50 or 1000 ppm manganese. The gut absorbs approximately 1% of this dietary manganese irrespective of its dietary concentration. The homeostasis of plasma manganese concentration must therefore be achieved by an excretory method. In vitro experiments have shown that manganese in plasma became bound to two proteins - to α2-macroglobulin, in its divalent Mn2+ state, and to transferrin, in its trivalent Mn3+ state. The proportions of 54Mn bound to these two proteins depended strongly on the temperature of incubation of 54Mn with plasma, and the temperature and pH at which electrophoresis was subsequently performed. 54Mn2+ was bound to transferrin only in the presence of an oxidizing agent such as molecular oxygen, ceruloplasmin or permanganate, and α2-macroglobulin was not involved in this process. In vivo experiments using cows with permanently indwelling mesenteric, portal and hepatic venous cannulae have shown that the liver cleared the portal blood quantitatively of free Mn2+ ions, removed approximately 75% of Mn2+ bound to α2-macroglobulin, but removed practically none of the Mn3+ transferrin complex. These results suggest that manganese may be absorbed through the gut as Mn2+ ions; some of these become bound to α2-macroblobulin while any excess free ions are extracted by the liver and excreted. Some of those bound to α2-macroglobulin enter the systemic circulation and are oxidized, either in the plasma or in tissue, to the Mn3+ state and become bound to transferring, the form in which manganese is circulated for metabolic purposes. (author)

  7. Early weaning alters the acute-phase reaction to an endotoxin challenge in beef calves.

    Science.gov (United States)

    Carroll, J A; Arthington, J D; Chase, C C

    2009-12-01

    Previous research indicates that early weaning before shipment can reduce transportation-induced increases in acute-phase proteins (APP) and can increase feedlot performance in beef calves. These data suggest that the combination of weaning and transport stress may compromise the immune system of calves, thus hindering subsequent performance and health. Therefore, our objective was to determine if the innate immune response of early weaned calves (EW; 80 d of age) differed from normal-weaned calves (NW; 250 d of age) in response to an endotoxin challenge. Eighteen Brahman x Angus calves (8 and 10 EW and NW, respectively; 233 +/- 5 kg of BW) were used. Calves were maintained on pasture with supplement and then moved into individual pens for 1 wk of acclimation before the start of the study. Calves were fitted with an indwelling jugular catheter 1 d before LPS challenge (0 h; 1.0 microg/kg of BW, intravenously). Blood samples were collected at 30-min intervals from -2 to 8 h. Serum samples were stored at -80 degrees C until analyzed for cortisol, tumor necrosis factor-alpha (TNF), IL-1 beta, IL-6, interferon-gamma (IFN), ceruloplasmin, and haptoglobin. Whereas LPS increased serum cortisol (P or= 0.15) was observed. A weaning age x time interaction (P x time interaction (P

  8. Valor de alguns exames complementares na Coréia de Sydenha

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    Aron J. Diament

    1972-09-01

    Full Text Available Sixty eight cases of Sydenham's chorea (SC were studied with the purpose of characterizing biologically the choreic individual by means of some laboratorial data. Based on antecedents, on the presence of recent infectious disease, on clinical examination, on electrocardiographs and x-rays of the heart, and according to a modified Jones criteria the patients were initially divided in three groups: aGroup 1 — 30 patients (case 1 to 30 which presented SC associated with active rheumatic fever (RF; b Group 2 — 20 patients (cases 31 to 50 which presented SC associated with a previou or present infectious state without active RF; c Group 3 — 18 patients (cases 51 to 68 which presented "pure" SC, not having anything in their antecedents, or in present history, nor in their physical examinations that could justify calling them "rheumatic" or "infectious". However the analysis of the clinical data, by means of the homogenization tests (qui square or the exact Fisher test and Goodman's contrast test showed the artificiality of this grouping, which could not be longer sustained. From the 68 cases studied the average age group was 9.9 years, with the maximum age being 17 years, and the minimum age being 4.5 years. 47 of the cases were females as compared to 21 males (2.2 to 1; 60 patients were white, 7 dark-skinned and one negro. The average evolution time of the choreic syndrome, at the time of the first consultation, was 6 months and 7 days, with a minimum of 13 days and a maximum of 60 months. The incidence of the outbreak as far as the season of the year is concerned, was as follows: 31 cases between autumn and winter; 14 cases in spring and 22 in summer. The following laboratory examinations have been made: a"classical acute phase serum reagents" (APSR: sedimentation rate, differential blood count, Weltmann reaction, mucoproteins, C reactive protein, antistreptolysin-O titter, electrophoresis of serum proteins; b copper and ceruloplasmin

  9. Valor de alguns exames complementares na Coréia de Sydenha The value of some laboratorial data in Sydenham's chorea

    Directory of Open Access Journals (Sweden)

    Aron J. Diament

    1972-09-01

    Full Text Available Sixty eight cases of Sydenham's chorea (SC were studied with the purpose of characterizing biologically the choreic individual by means of some laboratorial data. Based on antecedents, on the presence of recent infectious disease, on clinical examination, on electrocardiographs and x-rays of the heart, and according to a modified Jones criteria the patients were initially divided in three groups: aGroup 1 — 30 patients (case 1 to 30 which presented SC associated with active rheumatic fever (RF; b Group 2 — 20 patients (cases 31 to 50 which presented SC associated with a previou or present infectious state without active RF; c Group 3 — 18 patients (cases 51 to 68 which presented "pure" SC, not having anything in their antecedents, or in present history, nor in their physical examinations that could justify calling them "rheumatic" or "infectious". However the analysis of the clinical data, by means of the homogenization tests (qui square or the exact Fisher test and Goodman's contrast test showed the artificiality of this grouping, which could not be longer sustained. From the 68 cases studied the average age group was 9.9 years, with the maximum age being 17 years, and the minimum age being 4.5 years. 47 of the cases were females as compared to 21 males (2.2 to 1; 60 patients were white, 7 dark-skinned and one negro. The average evolution time of the choreic syndrome, at the time of the first consultation, was 6 months and 7 days, with a minimum of 13 days and a maximum of 60 months. The incidence of the outbreak as far as the season of the year is concerned, was as follows: 31 cases between autumn and winter; 14 cases in spring and 22 in summer. The following laboratory examinations have been made: a"classical acute phase serum reagents" (APSR: sedimentation rate, differential blood count, Weltmann reaction, mucoproteins, C reactive protein, antistreptolysin-O titter, electrophoresis of serum proteins; b copper and ceruloplasmin

  10. Acute phase response, inflammation and metabolic syndrome biomarkers of Libby asbestos exposure

    International Nuclear Information System (INIS)

    Identification of biomarkers assists in the diagnosis of disease and the assessment of health risks from environmental exposures. We hypothesized that rats exposed to Libby amphibole (LA) would present with a unique serum proteomic profile which could help elucidate epidemiologically-relevant biomarkers. In four experiments spanning varied protocols and temporality, healthy (Wistar Kyoto, WKY; and F344) and cardiovascular compromised (CVD) rat models (spontaneously hypertensive, SH; and SH heart failure, SHHF) were intratracheally instilled with saline (control) or LA. Serum biomarkers of cancer, inflammation, metabolic syndrome (MetS), and the acute phase response (APR) were analyzed. All rat strains exhibited acute increases in α-2-macroglobulin, and α1-acid glycoprotein. Among markers of inflammation, lipocalin-2 was induced in WKY, SH and SHHF and osteopontin only in WKY after LA exposure. While rat strain- and age-related changes were apparent in MetS biomarkers, no LA effects were evident. The cancer marker mesothelin was increased only slightly at 1 month in WKY in one of the studies. Quantitative Intact Proteomic profiling of WKY serum at 1 day or 4 weeks after 4 weekly LA instillations indicated no oxidative protein modifications, however APR proteins were significantly increased. Those included serine protease inhibitor, apolipoprotein E, α-2-HS-glycoprotein, t-kininogen 1 and 2, ceruloplasmin, vitamin D binding protein, serum amyloid P, and more 1 day after last LA exposure. All changes were reversible after a short recovery regardless of the acute or long-term exposures. Thus, LA exposure induces an APR and systemic inflammatory biomarkers that could have implications in systemic and pulmonary disease in individuals exposed to LA. -- Highlights: ► Biomarkers of asbestos exposure are required for disease diagnosis. ► Libby amphibole exposure is associated with increased human mortality. ► Libby amphibole increases circulating proteins involved

  11. Obesidad y su relación con marcadores de inflamación y ácidos grasos de eritrocito en un grupo de adolescentes obesos Obesity and its relation with markers of inflammation and erythrocyte fatty acids in a group of overweight adolescents

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    M. J. Aguilar Cordero

    2012-02-01

    Full Text Available En la actualidad se acepta que la obesidad constituye un proceso eminentemente inflamatorio. Ello tiene su justificación en las alteraciones descritas en sujetos obesos a nivel de la secreción de ciertas citoquinas tales como ceruloplasmina, leptina, adiponectina e interleuquina 6 (IL-6, entre otras. Asimismo y en modo paralelo, otros trabajos apuntan además hacia alteraciones en la composición de ácidos grasos en la membrana de los eritrocitos de adultos obesos. Los resultados obtenidos en este estudio confirman la existencia de una correlación significativa entre los niveles séricos de algunas de las citokinas estudiadas y el estado nutricional de los sujetos, lo que significa que para la población de escolares valorada, las concentraciones séricas de estas biomoléculas pueden constituir una importante herramienta para predecir el riesgo cardiovascular en la edad adulta. Además, se han encontrado diferencias significativas respecto de la composición en ácidos grasos saturados en la membrana de los eritrocitos.There is a general consensus that obesity is an eminently inflammatory process. This is justified by alterations observed in obese patients, which affect the secretion of certain cytokines such as ceruloplasmin, leptin, adiponectin, and interleukin-6 (IL-6, among others. In a parallel way, other research has also pointed out alterations in the composition of fatty acids in the erythrocyte membrane of overweight adults. The results obtained in our study confirm the existence of a significant correlation between the serum levels of some of the cytokines studied and the nutritional state of the sample studied. This means that for the population of children evaluated in our study, the serum concentrations of these biomolecules can be an important tool for the prediction of cardiovascular risk when they become adults. Furthermore significant differences were found regarding the composition of saturated fatty acids in the

  12. Micronutrientes e capacidade antioxidante em adolescentes sedentários e corredores Micronutrients and antioxidant capacity in sedentary adolescents and runners

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    Karla de Jesus Fernandes de Oliveira

    2007-04-01

    Full Text Available OBJETIVO: Este estudo objetivou comparar a composição corporal, a ingestão dietética, os índices bioquímicos de micronutrientes antioxidantes e a capacidade antioxidante em adolescentes sedentários (n=15 e corredores (n=18, pós-púberes. MÉTODOS: A composição corporal foi aferida por meio das dobras cutâneas, massa corporal total e estatura; a ingestão de micronutrientes foi determinada através de freqüência de consumo alimentar e os indicadores bioquímicos por coleta de sangue em jejum. Em sangue total foram determinados hematócrito e hemoglobina; em plasma, testosterona, alfa-tocoferol, cobre, zinco, e ceruloplasmina; em eritrócitos, fragilidade osmótica, zinco, Cu-Zn superóxido dismutase e metalotioneína. RESULTADOS: A capacidade antioxidante, a ingestão dietética e a composição corporal foram similares, exceto o somatório de dobras cutâneas, que foi menor nos corredores (pOBJECTIVE: The aim of this study was to compare body composition, biochemical indices of antioxidant micronutrients, intake and nutritional status and antioxidant capacity in post-puberty sedentary adolescents (n=15 and runners (n=18. METHODS: Skin-fold measurements, total body mass and height were used for anthropometric evaluation and a food frequency questionnaire for assessment of micronutrient intake. Biochemical indices measured after an overnight fast included: blood hemoglobin and hematocrit; plasma testosterone, a-tocopherol, copper, zinc and ceruloplasmin; and in erythrocytes, osmotic fragility, zinc, Cu-Zn superoxide dismutase and metallothionein. RESULTS: Indices of antioxidant capacity, dietary intake, and body composition were not different between the groups, except for the sum of skin-folds that was lower in runners (p<0.05. Most adolescents had vitamin E intakes lower than nutritional recommendations. Plasma levels of copper and zinc were, on average, adequate. Copper levels were similar in both groups and zinc levels were higher

  13. Studies on serum protein fractions of patients with maxillary sinus cancer undergoing a combination of radiotherapy and chemotherapy

    International Nuclear Information System (INIS)

    39 cases of maxillary sinus cancer were treated with combined intra-arterial infusion of bleomycin and external irradiation of 60Coγ-ray. Serum protein fractions in the sera of 39 cases of maxillary sinus cancer were examined through the radial immunodiffusion method, 1) before radiation treatment was given, 2) at the completion of radiation therapy and 3) after radiation therapy, ranging from 1 month to 36 months. The sera obtained from patients with maxillary sinus cancer before treatment had concentrations of: α1-acid glycoprotein (α1AG), α1-antitrypsin (α1AT), α1-chymotrypsin (α1X), haptoglobin (Hp), ceruloplasmin (Cp), β1C/A glycoprotein (C3), hemopexin (Hx), IgG and of IgA which were elevated compared with those of normal controls: However, the serum concentrations of: prealbumin (Prealb), albumin (Alb), transferrin (Tf), α2-macroglobulin (α2M), HS-glycoprotein (α2HS) and of IgM were reduced significantly. At the completion of radiation therapy, the concentrations of: Prealb, Tf, α1AG, α1X, Hx, C3, IgG, IgA and of IgM rose slightly as compared with levels before radiation therapy, and the concentrations of Alb, of α2HS and of α1AT were reduced. However, there was no significant difference between the former and the latter data. In patients who died within 12 months after treatment, the Prealb, Alb, Tf, α2HS, and IgM levels examined before radiation therapy were reduced and the α1AG level was elevated compared with those who had lived more than 13 months. The Prealb, Alb, Tf, and IgM levels, after radiation therapy were reduced; while the α1AG, Hp, and α1X levels were elevated. In patients who had lived more than 13 months, after treatment, concentrations of Prealb, of Alb, of Tf, of C3, of Hx, of IgG, of IgA and of IgM rose before and after radiation therapy, compared with those who died within 12 months. (author)

  14. Global serum glycoform profiling for the investigation of dystroglycanopathies & Congenital Disorders of Glycosylation.

    Science.gov (United States)

    Heywood, Wendy E; Bliss, Emily; Mills, Philippa; Yuzugulen, Jale; Carreno, Gabriela; Clayton, Peter T; Muntoni, Francesco; Worthington, Viki C; Torelli, Silvia; Sebire, Neil J; Mills, Kevin; Grunewald, Stephanie

    2016-06-01

    The Congenital Disorders of Glycosylation (CDG) are an expanding group of genetic disorders which encompass a spectrum of glycosylation defects of protein and lipids, including N- & O-linked defects and among the latter are the muscular dystroglycanopathies (MD). Initial screening of CDG is usually based on the investigation of the glycoproteins transferrin, and/or apolipoprotein CIII. These biomarkers do not always detect complex or subtle defects present in older patients, therefore there is a need to investigate additional glycoproteins in some cases. We describe a sensitive 2D-Differential Gel Electrophoresis (DIGE) method that provides a global analysis of the serum glycoproteome. Patient samples from PMM2-CDG (n = 5), CDG-II (n = 7), MD and known complex N- & O-linked glycosylation defects (n = 3) were analysed by 2D DIGE. Using this technique we demonstrated characteristic changes in mass and charge in PMM2-CDG and in charge in CDG-II for α1-antitrypsin, α1-antichymotrypsin, α2-HS-glycoprotein, ceruloplasmin, and α1-acid glycoproteins 1&2. Analysis of the samples with known N- & O-linked defects identified a lower molecular weight glycoform of C1-esterase inhibitor that was not observed in the N-linked glycosylation disorders indicating the change is likely due to affected O-glycosylation. In addition, we could identify abnormal serum glycoproteins in LARGE and B3GALNT2-deficient muscular dystrophies. The results demonstrate that the glycoform pattern is varied for some CDG patients not all glycoproteins are consistently affected and analysis of more than one protein in complex cases is warranted. 2D DIGE is an ideal method to investigate the global glycoproteome and is a potentially powerful tool and secondary test for aiding the complex diagnosis and sub classification of CDG. The technique has further potential in monitoring patients for future treatment strategies. In an era of shifting emphasis from gel- to mass-spectral based proteomics

  15. Effect of dietary trace mineral concentration and source (inorganic vs. chelated) on performance, mineral status, and fecal mineral excretion in pigs from weaning through finishing.

    Science.gov (United States)

    Creech, B L; Spears, J W; Flowers, W L; Hill, G M; Lloyd, K E; Armstrong, T A; Engle, T E

    2004-07-01

    Two hundred and sixteen weanling gilts (6.65+/-0.08 kg) were used to determine the effects of decreasing supplemental concentrations of Zn, Cu, Fe, and Mn, and trace mineral source (inorganic vs. chelated) on growth performance, mineral status, and fecal mineral concentrations from weaning through development. The study was conducted over three trials with 72 pigs in each trial. Gilts were blocked by weight and randomly assigned to either 1) control, 2) reduced inorganic, or 3) reduced chelated trace minerals. The control diet was supplemented with 25, 150, 180, and 60 mg/kg of Cu, Zn, Fe, and Mn (in sulfate forms), respectively, during the nursery phase and 15, 100, 100, and 40 mg/kg of supplemental Cu, Zn, Fe, and Mn, respectively, during the growing and gilt-developer phases. Reduced inorganic and reduced chelated treatments were supplemented during all phases with 5, 25, 25, and 10 mg/kg of Cu, Zn, Fe, and Mn, respectively. The reduced chelated treatment supplied 50% of the supplemental Cu, Zn, Fe, and Mn in the form of metal proteinates, with the remainder from sulfate forms. Performance by control pigs did not differ from pigs fed the reduced trace mineral treatments during the nursery and grower-development periods. Gain:feed was lower (P < 0.05) for pigs fed the reduced inorganic compared with those fed the reduced chelated treatment during the nursery period. Trace mineral source did not affect performance during the growing or gilt-developer phase. Plasma Zn concentration and alkaline phosphatase activity were higher (P < 0.01) in control pigs than in those receiving reduced trace minerals during the nursery and growing phases. Plasma Cu concentration and ceruloplasmin activity were generally not affected by treatment. Hemoglobin concentrations were lower (P < 0.05) for the reduced inorganic compared with the reduced chelated treatment in the nursery phase. Fecal concentrations of Cu, Zn, and Mn were lower (P < 0.05) in pigs fed reduced trace minerals

  16. Acute phase response, inflammation and metabolic syndrome biomarkers of Libby asbestos exposure

    Energy Technology Data Exchange (ETDEWEB)

    Shannahan, Jonathan H. [Curriculum in Toxicology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599 (United States); Alzate, Oscar [Systems Proteomics Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599 (United States); Winnik, Witold M.; Andrews, Debora [Proteomics Core, Research Core Unit, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711 (United States); Schladweiler, Mette C. [Cardiopulmonary and Immunotoxicology Branch, Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711 (United States); Ghio, Andrew J. [Clinical Research Branch, Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Chapel Hill, NC 27599 (United States); Gavett, Stephen H. [Cardiopulmonary and Immunotoxicology Branch, Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711 (United States); Kodavanti, Urmila P., E-mail: Kodavanti.Urmila@epa.gov [Cardiopulmonary and Immunotoxicology Branch, Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711 (United States)

    2012-04-15

    Identification of biomarkers assists in the diagnosis of disease and the assessment of health risks from environmental exposures. We hypothesized that rats exposed to Libby amphibole (LA) would present with a unique serum proteomic profile which could help elucidate epidemiologically-relevant biomarkers. In four experiments spanning varied protocols and temporality, healthy (Wistar Kyoto, WKY; and F344) and cardiovascular compromised (CVD) rat models (spontaneously hypertensive, SH; and SH heart failure, SHHF) were intratracheally instilled with saline (control) or LA. Serum biomarkers of cancer, inflammation, metabolic syndrome (MetS), and the acute phase response (APR) were analyzed. All rat strains exhibited acute increases in α-2-macroglobulin, and α1-acid glycoprotein. Among markers of inflammation, lipocalin-2 was induced in WKY, SH and SHHF and osteopontin only in WKY after LA exposure. While rat strain- and age-related changes were apparent in MetS biomarkers, no LA effects were evident. The cancer marker mesothelin was increased only slightly at 1 month in WKY in one of the studies. Quantitative Intact Proteomic profiling of WKY serum at 1 day or 4 weeks after 4 weekly LA instillations indicated no oxidative protein modifications, however APR proteins were significantly increased. Those included serine protease inhibitor, apolipoprotein E, α-2-HS-glycoprotein, t-kininogen 1 and 2, ceruloplasmin, vitamin D binding protein, serum amyloid P, and more 1 day after last LA exposure. All changes were reversible after a short recovery regardless of the acute or long-term exposures. Thus, LA exposure induces an APR and systemic inflammatory biomarkers that could have implications in systemic and pulmonary disease in individuals exposed to LA. -- Highlights: ► Biomarkers of asbestos exposure are required for disease diagnosis. ► Libby amphibole exposure is associated with increased human mortality. ► Libby amphibole increases circulating proteins involved

  17. Competition between copper and silver in Fischer rats with a normal copper metabolism and in Long-Evans Cinnamon rats with an abnormal copper metabolism.

    Science.gov (United States)

    Sugawara, N; Sugawara, C

    2000-07-01

    Long-Evans Cinnamon (LEC) rats inherently lacking in serum ceruloplasmin (CP) activity and biliary Cu excretion were established from a closed colony of Long-Evans rats. These deficiencies, linked to a dysfunction of P-type ATPase, stimulate deposition of Cu and then of Cu metallothionein (MT) in the liver. Male LEC and Fischer rats were injected subcutaneously with Ag (AgNO3), which is an antagonist to Cu. They were operated on 24 h after the injection while under anesthesia. Total uptake of Ag into the liver was not stimulated, but its uptake into the MT fraction increased significantly in the LEC rats. Ag injection notably decreased the activity of serum CP in the Fischer rats, but not in the LEC rats. The decrease was accompanied by a reduction of serum Cu. In Fischer rat serum treated with Ag, Ag was detected mainly in the albumin region and partly in the CP fraction. In LEC rat serum, however, the Ag concentration was about 1/20 of that in the Fischer rats, and Ag was not detected in the CP fraction. Ag injection decreased the biliary excretion of Cu in the Fischer rats (0.183-0.052 microg Cu/20 min sampling), but not in the LEC rats (0.014-0.014 microg Cu/20 min sampling). On the other hand, biliary excretion of Ag was much greater in the Fischer rats (1.25 microg Ag/20 min) than in the LEC rats (0.04 microg Ag/20 min). Our results suggest that uptake of Ag into the liver is not dependent on the hepatic Cu content and status, but that biliary excretion of Ag from the liver is affected by these. Hepatic MT is not a transporter of hepatobiliary excretion of Cu and Ag. It seems likely that, unlike Cu excretion, Ag is excreted by not only the CP route but also by another route into the serum. Ag may compete with Cu in the uptake into CP (conversion of apo-CP to holo-CP). PMID:10959791

  18. Alteration of biochemical parameters after supplementation of multivitamins and minerals of sprayers on grape gardens of Western Maharashtra (India

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    Jyotsna A. Patil

    2014-01-01

    , Albumin, Globulin, A/G ratio and Ceruloplasmin were not altered significantly as compared to before vitamin A to Z and multi minerals supplementation. Conclusions: Alteration of these biochemical parameters may be due to ameliorating effects of vitamin A to Z and multi minerals. The vitamins or minerals present in A to Z tablets may improve the hepatocellular damage caused by various pesticides, also beneficial for either decrease free radical formation or increased antioxidant status and increased AChE level may be decreased inhibition of AChE by pesticides of sprayers of grape gardens.

  19. Epilepsy secondary to Menkes' disease: five cases report and review of literature

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    Pei-yuan ZHANG

    2014-12-01

    Full Text Available Objective To study the clinical features of patients with Menkes' disease (MD that initiate from seizures, and to reinforce the knowledge of etiological diagnosis of secondary epilepsy.  Methods The clinical and laboratory features of 5 MD patients who were admitted from December 1992 to March 2014 were retrospectively analyzed.  Results All cases were male infants including two brothers, and the age of onset was 1-5 months after birth. Their clinical manifestations started from tonic (N = 3 and focal clonic (N = 2 seizures. The focal clonic seizures became more frequent and progressively worsened. Four cases showed abnormal background EEG rhythm; 3 cases revealed interictal focal or multifocal epileptiform discharges over occipital region predominantly; 2 cases revealed epileptic discharge in fit period EEG. All the cases manifested severe mental retardation accompanied with light complexion and curly hair. Low serum copper and ceruloplasmin levels were found in 4 cases and lead to the diagnosis of MD. The exceptional one was made clinical diagnosis of MD due to his typical manifestation and the diagnosis of his elder brother. Three cases received MRI examination, which showed extensive cerebral atrophy and symmetric encephalomalacia foci. One case revealed tortuosity of main artery and diminution of distal artery by MRA. One case was treated with phenobarbital, 2 cases with topiramate, one case with topiramate and levetiracetam and clonazepam. One case died of respiratory failure at 24 h after admission. The other 4 cases were followed up for 1-9 months after being discharged, 3 of whom died, and the left one converted to intractable infantile spasms and severe mental retardation, with interictal EEG changing to hypsarrhythmia.  Conclusions The onset of patients with epilepsy secondary to MD often occurs in early infancy, manifesting tonic and focal clonic seizures predominantly with interictal epileptiform discharges over the posterior

  20. The effect of early weaning on feedlot performance and measures of stress in beef calves.

    Science.gov (United States)

    Arthington, J D; Spears, J W; Miller, D C

    2005-04-01

    Forty crossbred steers (Brahman x English) were categorized into two groups: 1) early weaned (EW; n = 20); and 2) normal weaned (NW; n = 20). Calves were 89 and 300 d of age at the time of EW and NW, respectively; SEM = 4.4. Early-weaned calves were kept on-site (University of Florida, Ona), provided supplement (1% of BW), and grazed on annual and perennial pastures until NW. At the time of normal weaning, all calves were loaded on a commercial livestock trailer and transported to the North Carolina State University Research Feedlot in Butner (approximately 1,200 km). Upon arrival, calves were stratified by BW and randomly allotted to four pens per weaning age treatment. Individual calf BW and blood samples were collected at the time of normal weaning, on arrival at the feedlot (d 1; 24 h following weaning), and on d 3, 7, 14, 21, and 28 of the receiving period. Individual BW was collected at the start and end of the growing and finishing periods, and feed intake by pen was measured daily. As an estimate of stress during the receiving period, plasma was collected and analyzed for the acute-phase proteins, haptoglobin and ceruloplasmin. Early-weaned calves were lighter (P = 0.03) at normal weaning than NW calves (221 vs. 269 kg; SEM = 10.6). By d 28, EW calves tended (P = 0.12) to be lighter than NW calves (242 vs. 282 kg, respectively). Gain:feed was improved for EW compared with NW calves during both the receiving (G:F = 0.157 vs. 0.081) and growing (0.159 vs. 0.136) periods. There tended (P yield grade, marbling score, and LM area, did not differ between treatments. These data imply that EW calves, which are maintained onsite before shipping, may be more tolerant to the stressors associated with transportation and feed yard entry. Early weaned calves, managed within the system described in this study, may have improved G:F. PMID:15753350

  1. Stay-green ranking and maturity of corn hybrids: 2. Effects on the performance of lactating dairy cows.

    Science.gov (United States)

    Arriola, K G; Kim, S C; Staples, C R; Adesogan, A T

    2012-02-01

    ceruloplasmin concentration, and increased the efficiency of milk production. Except for increasing yeast and mold counts, adding water to the HSG hybrid harvested at 27% DM did not have adverse effects but was associated with greater starch intake, CP digestibility, and ruminal total VFA concentration, and decreased acetate to propionate ratio in dairy cows. PMID:22281361

  2. Ocular copper deposition associated with monoclonal gammopathy of undetermined significance: case report Depósito ocular de cobre associado a gamopatia monoclonal de significância indeterminada: relato de caso

    Directory of Open Access Journals (Sweden)

    Patrick F. Tzelikis

    2005-08-01

    Full Text Available To report a case of ocular copper deposition in both eyes at the level of Descemet's membrane associated with a monoclonal gammopathy of undetermined significance (MGUS. A 49-year-old white woman had golden-brown metallic dust-like deposits on Descemet's membrane of both eyes. A systemic examination revealed an elevated serum copper, normal serum ceruloplasmin and a normal level of total protein. Serum protein electrophoresis demonstrated a single peak (M-spike in the gamma region (M-protein in serum = 11 g/l. Flow cytometric analysis of the marrow aspirate identified a monoclonal plasma cell population that represents approximately 2% of the total marrow cells consistent with the diagnosis of monoclonal gammopathy of undetermined significance. Copper deposits at the level of Descemet's membrane may be a finding in a patient with monoclonal gammopathy of undetermined significance.Relatar um caso de depósito corneano de cobre em ambos os olhos a nível da membrana de Descemet associado a gamopatia monoclonal de significância indeterminada (GMSI. Paciente feminina, 49 anos, leucodérmica, apresentando depósito corneano de aspecto marrom-ouro a nível da membrana de Descemet em ambos os olhos. Exame sistêmico revelou cobre sérico elevado, ceruloplasmina sérica normal, e proteína total normal. Eletroforese de proteínas séricas demonstrou um pico único na região gama (proteína M = 11 g/l. Análise citométrica de aspirado medular evidenciou uma população de células plasmáticas monoclonais de aproximadamente 2% do total de células medulares consistente com o diagnóstico de gamopatia monoclonal de significância indeterminada. Depósitos de cobre a nível da membrana de Descemet podem ser encontrados em pacientes com gamopatia monoclonal de significância indeterminada.

  3. Involvement of activated leukocytes in the regulation of plasma levels of acute phase proteins in microgravity simulation experiments

    Science.gov (United States)

    Larina, Olga; Bekker, Anna; Turin-Kuzmin, Alexey

    2016-07-01

    Earth-based studies of microgravity effects showed the induction of the mechanisms of acute phase reaction (APR). APR comprises the transition of stress-sensitive protein kinases of macrophages and other responsive cells into the active state and the phosphorylation of transcription factors which in turn stimulate the production of acute-phase reaction cytokines. Leukocyte activation is accompanied by the acceleration of the formation of oxygen radicals which can serve a functional indice of leukocyte cell state. The series of events at acute phase response result in selective changes in the synthesis of a number of secretory blood proteins (acute phase proteins, APPs) in liver cells thus contributing the recovery of homeostasis state in the organism. Earlier experiment with head-down tilt showed the increase in plasma concentrations of two cytokine mediators of acute phase response, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) being the outcome of the activation of producer cells, foremost, leukocytes. In experiment with 4-day dry immersion chemiluminescent (ChL) reply of the whole blood samples to a test stimulus were studied along with the measurements of plasma levels of APPs, namely, alpha1-antitrypsin (alpha1-AT), alpha1-acid glycoprotein (alpha1-AGP), alpha2-macroglobulin (alpha2-M), ceruloplasmin (Cer), haptoglobin (Hp), C3-complement component (C3), C-reactive protein (CRP). Eight individuals aged 21.2 ± 3.2 years were the test subjects in the investigation. Protein studies showed a noticeable increase in the mean plasma levels of all APPs measured in experiment thus producing the evidence of the activation of acute phase response mechanisms while individual patterns revealed variability during the immersion period. The overall trends were similar to these in the previous immersion series. The augment in the strength of signal in stimulated light emission tests was higher after 1- and 2-day of immersion exposure than before the

  4. Maternal rumen-protected methionine supplementation and its effect on blood and liver biomarkers of energy metabolism, inflammation, and oxidative stress in neonatal Holstein calves.

    Science.gov (United States)

    Jacometo, C B; Zhou, Z; Luchini, D; Trevisi, E; Corrêa, M N; Loor, J J

    2016-08-01

    In nonruminants, nutrition during pregnancy can program offspring development, metabolism, and health in later life. Rumen-protected Met (RPM) supplementation during the prepartum period improves liver function and immune response in dairy cows. Our aim was to investigate the effects of RPM during late pregnancy on blood biomarkers (23 targets) and the liver transcriptome (24 genes) in neonatal calves from cows fed RPM at 0.08% of diet dry matter/d (MET) for the last 21 d before calving or controls (CON). Blood (n=12 calves per diet) was collected at birth before receiving colostrum (baseline), 24 h after receiving colostrum, 14, 28, and 50 d (post-weaning) of age. Liver was sampled (n=8 calves per diet) via biopsy on d 4, 14, 28, and 50 of age. Growth and health were not affected by maternal diet. The MET calves had greater overall plasma insulin concentration and lower glucose and ratios of glucose-to-insulin and fatty acids-to-insulin, indicating greater systemic insulin sensitivity. Lower concentration of reactive oxygen metabolites at 14 d of age along with a tendency for lower overall concentration of ceruloplasmin in MET calves indicated a lesser degree of stress. Greater expression on d 4 of fructose-bisphosphatase 1 (FBP1), phosphoenolpyruvate carboxykinase 1 (PCK1), and the facilitated bidirectional glucose transporter SLC2A2 in MET calves indicated alterations in gluconeogenesis and glucose uptake and release. The data agree with the greater expression of the glucocorticoid receptor (GR). Greater expression on d 4 of the insulin receptor (INSR) and insulin-responsive serine/threonine-protein kinase (AKT2) in MET calves indicated alterations in insulin signaling. In that context, the similar expression of sterol regulatory element-binding transcription factor 1 (SREBF1) in CON and MET during the preweaning period followed by the marked upregulation regardless of diet after weaning (d 50) support the idea of changes in hepatic insulin sensitivity during

  5. Mortes súbitas em bovinos associadas à carência de cobre Sudden deaths in cattle associated with copper deficiency

    Directory of Open Access Journals (Sweden)

    Ana Paula Marques

    2003-01-01

    -d.m. and S (0.2%±0.03-d.m., but high levels of Fe (522±122 ppm-d.m.. One group of 10 heifers was supplemented with Cu subcutaneously. This group and a similar control group were maintained in the same area where the outbreak occurred. Serum levels of Cu, S, Fe, Mo and ceruloplasmin were determined bimonthly in both groups during one year. Mean serum levels of Cu (1.76±1.06 to 10.34±3.1 µmol/l for the control group and 3.86±1.53 to 10.61±1.34 µmol/l for the treated group and ceruloplasmin (6.59±3.93 to 18.61±4.14 mg/l for the control group and 10.31±5.48 to 32.49±6.05 mg/l for the treated group were significantly higher in the supplemented group (P=0.0046 for Cu and P=0.0001 for ceruloplasmin, but they were below normal levels in most samples of both groups. Serum levels of Cu and ceruloplasmin were correlated (r=0.67; P=00.5. In both groups serum levels of Fe (40.09±5.25 to 78.48±28.23 µmol/l were higher than normal levels. Samples of forage were collected bimonthly for determination of Cu, Mo, S, Fe and protein in 7 points of the paddock where the outbreak occurred. Levels of Cu (1.36±0.56 to 4.76±1.15 ppm-d.m. were below the normal ranges. The concentration of Mo (0.17±0.06 to 0.96±0.47 ppm-d.m. was within normal ranges. Levels of S (0.21±0.04% to 0.5±0.17% and Fe (172.92±62.64 to 437.24±205.44 ppm-d.m. were occasionally within toxic level. Levels of protein varied from 7.77±2.6% to 13.16±3.02%. Six samples of water and six of submersed pastures were collected at the end of the experiment when the paddock was flooded. High levels of iron (169.23±83.49 ppm-d.m. and S (0.06±0.03% were found in the water. Levels of Fe and S in the submerse pasture were of 469.3±218.28 ppm and 0.19±0.05%, respectively. The results obtained suggest that the sudden deaths are due to Cu deficiency. Three factors seem to be responsible for the Cu deficiency: 1 high levels of iron in pastures and water; 2 occasionally low levels of copper in the pastures; and 3 the

  6. Drug- not carrier-dependent haematological and biochemical changes in a repeated dose study of cyclosporine encapsulated polyester nano- and micro-particles: Size does not matter

    International Nuclear Information System (INIS)

    Highlights: • The particulate delivery allows an increase in dose range without accrual of toxicities. • The altered haematological and biochemical changes are drug, but not particle dependent. • PLGA nano/microparticles are safe on subacute peroral dosing over 28 days. • Nano-toxicology, drug needs to be considered. - Abstract: Biodegradable nanoparticles are being considered more often as drug carriers to address pharmacokinetic/pharmacodynamic issues, yet nano-product safety has not been systematically proven. In this study, haematological, biochemical and histological parameters were examined on 28 day daily dosing of rats with nano- or micro-particle encapsulated cyclosporine (CsA) to confirm if any changes observed were drug or carrier dependent. CsA encapsulated poly(lactide-co-glycolide) [PLGA] nano- (nCsA) and micro-particles (mCsA) were prepared by emulsion techniques. CsA (15, 30, 45 mg/kg) were administered by oral gavage to Sprague Dawley (SD) rats over 28 days. Haematological and biochemical metrics were followed with tissue histology performed on sacrifice. Whether presented as nCsA or mCsA, 45 mg/kg dose caused significant loss of body weight and lowered food consumption compared to untreated control. Across the doses, both nCsA and mCsA produce significant decreases in lymphocyte numbers compared to controls, commensurate with the proprietary product, Neoral® 15. Dosing with nCsA showed higher serum drug levels than mCsA presumably owing to the smaller particle size facilitating absorption. The treatment had no noticeable effects on inflammatory/oxidative stress markers or antioxidant enzyme levels, except an increase in ceruloplasmin (CP) levels for high dose nCsA/mCsA group. Further, only subtle, sub-lethal changes were observed in histology of nCsA/mCsA treated rat organs. Blank (drug-free) particles did not induce changes in the parameters studied. Therefore, it is extremely important that the encapsulated drug in the nano-products is

  7. Effects of occupational exposure to tobacco smoke: is there a link between environmental exposure and disease?

    Science.gov (United States)

    Pacheco, Solange A; Torres, Vukosava M; Louro, Henriqueta; Gomes, Filomena; Lopes, Carlos; Marçal, Nelson; Fragoso, Elsa; Martins, Carla; Oliveira, Cátia L; Hagenfeldt, Manuela; Bugalho-Almeida, António; Penque, Deborah; Simões, Tânia

    2013-01-01

    In a previous study, evidence was provided that indoor secondhand tobacco smoke (SHS) air pollution remains high in Lisbon restaurants where smoking is allowed, regardless of the protective measures used. The aim of this study was to determine in these locations the levels of polycyclic aromatic hydrocarbons (PAH) associated with the particulate phase of SHS (PPAH), a fraction that contains recognized carginogens, such as benzo[a]pyrene (BaP). Data showed that restaurant smoking areas might contain PPAH levels as high as 110 ng/m(3), a value significantly higher than that estimated for nonsmoking areas (30 ng/m(3)) or smoke-free restaurants (22 ng/m(3)). The effective exposure to SHS components in restaurant smoking rooms was confirmed as cotinine levels found in workers' urine. Considering that all workers exhibited normal lung function, eventual molecular changes in blood that might be associated with occupational exposure to SHS and SHS-associated PPAH were investigated by measurement of two oxidative markers, total antioxidant status (TAS) and 8-hydroxyguanosine (8-OHdG) in plasma and serum, respectively. SHS-exposed workers exhibited higher mean levels of serum 8-OHdG than nonexposed workers, regardless of smoking status. By using a proteomics approach based on 2D-DIGE-MS, it was possible to identify nine differentially expressed proteins in the plasma of SHS-exposed nonsmoker workers. Two acute-phase inflammation proteins, ceruloplasmin and inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), were predominant. These two proteins presented a high number of isoforms modulated by SHS exposure with the high-molecular-weight (high-MW) isoforms decreased in abundance while low-MW isoforms were increased in abundance. Whether these expression profiles are due to (1) a specific proteolytic cleavage, (2) a change on protein stability, or (3) alterations on post-translational modification pattern of these proteins remains to be investigated. Considering that these

  8. Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy Leucograma e concentrações séricas de proteínas de fase aguda de gatas submetidas à ovariectomia convencional ou por videolaparoscopia

    Directory of Open Access Journals (Sweden)

    A.E. Alves

    2010-02-01

    Full Text Available Thirty health queens were submitted to ovariectomy by conventional technique or by videolaparoscopy. In order to study the intensity of inflammatory response by means of acute phase protein analysis and white blood cell count, serum samples were taken before and until 144 hours after the surgical procedures. The protein concentrations that were significantly increased 24 hours after surgical procedures were: ceruloplasmin, hemopexin, haptoglobin, and α1-acid glycoprotein, 69.8%, 103.5%, 117.3%, and 199.0%, respectively, for conventional ovariectomy; and 22.3%, 46.1%, 79.8%, and 74.6%, respectively, for laparoscopic ovariectomy. Therefore, inflammatory response was more intense in queens submitted to conventional ovariectomy. Results indicate that the increase or decrease in acute phase proteins, as well as in white blood cells count, may be useful in the evaluation of inflammatory response induced by these surgical procedures.Trinta gatas, saudáveis, foram submetidas à ovariectomia pela técnica convencional e por videolaparoscopia. Amostras de sangue foram obtidas com o objetivo de verificar a intensidade da resposta inflamatória por meio da análise das concentrações de proteinas de fase aguda e contagem de leucócitos antes e até 144 horas após procedimento cirúrgico. As proteínas que apresentaram aumento significativo 24 horas após a cirurgia foram: ceruloplasmina, hemopexina, haptoglobina e α1-glicoproteína ácida, 69,8%, 103,5%, 117,3% e 199,0%, respectivamente, para ovariectomia convencional, e 22,3%, 46,1%, 79,8% e 74,6%, respectivamente, para ovariectomia por videolaparoscopia. A resposta inflamatória foi mais evidente nas gatas submetidas à ovariectomia convencional. Os resultados mostram aumento e diminuição na concentração de proteínas de fase aguda e na contagem de leucócitos, podendo ser utilizados na avaliação da resposta inflamatória induzida por procedimentos cirúrgicos.

  9. Proteinograma sérico de bovinos da raça Curraleir Serum protein concentration in Curraleiro bovine breed

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    R.S. Juliano

    2009-06-01

    Full Text Available Estudou-se o perfil eletroforético das proteínas séricas de bovinos sadios da raça Curraleiro por meio da técnica de eletroforese em gel de acrilamida contendo dodecil sulfato de sódio. Utilizaram-se amostras de soro sanguíneo de 228 bovinos da raça Curraleiro, 51 machos e 177 fêmeas, com idades entre sete meses e 12 anos, pertencentes a dois rebanhos localizados nos Estados de Goiás e Tocantins. Foram quantificadas proteína total e concentração plasmática de fibrinogênio. Verificaram-se variações nas concentrações das diferentes frações proteicas. Foram detectadas 26 proteínas e identificadas 10 delas. A ceruloplasmina esteve ausente em 78,1% dos indivíduos, e a α-antitripsina não foi detectada em nenhum animal. Proteína total, globulina, IgA, IgG e fibrinogênio aumentaram com a idade e houve correlação positiva entre os níveis séricos de haptoglobina e α1-glicoproteína ácida.The eletrophoretic serum protein profile in healthy Curraleiro bovine breed was studied by dodecyl sulphate-polyacrylamide gel electrophoresis. A total of 228 serum samples from Curraleiro cattle, being 51 males and 177 females were analyzed. They were from seven month to 12-year-old and were raised in two farms of Goiás and Tocantins states. Total protein and plasma fibrinogen quantification were performed. It was possible to verify variation in proteins fractions concentration. Twenty-six proteins were detected and ten of them were identified. Ceruloplasmin was absente in 78,10% of animals and α-antitrypsin was not detected. The total protein, globulin, IgA, IgG, and fibrinogen increased with age and there was a positive correlaction between haptoglobin and α1-acid glycoprotein.

  10. Inflammatory mediators and immune response in Mexican adolescents Mediadores inflamatorios y respuesta inmunitaria en adolescentes mexicanos

    Directory of Open Access Journals (Sweden)

    R. V. Pardo Morales

    2011-10-01

    Full Text Available Introduction: Low-grade inflammation and increased immunity related to cardiovascular diseases have been described in children and adults, however, studies in Mexican adolescents are being done at present. Objective: To evaluate inflammatory proteins and indicators of immunity in adolescents by gender and body mass index. Material and methods: 115 Mexican adolescents, 15-18 years old (36 men, were divided into non-overweight, risk of overweight and overweight by CDC pediatric criteria by body mass index. Serum concentrations of ceruloplasmin, C3 and C4 were quantified by nephelometry; IL-6 and TNF-α from stimulated supernatant were analyzed with Human Th1-Th2 cytokine CBA II kit (BD Biosciences Pharmigen, San Diego, CA, and detected by flow cytometry. Data were analysed by Mann-Whitney U. Results: Gender differences were found in C3 (men: median 118.8, mean rank: 41.0; women: median: 143.9, mean rank: 65.7, p = 0.001 and ceruloplasmin (men: median: 31.01, mean rank: 47.06; women: median: 31.0, mean rank: 62.9, p = 0.015. Differences by BMI were found in C3 (women non-overweight: median: 137.00 mena rank: 36.52; women with risk of overweight/overweight: median: 175.80, mean rank: 57.69, p = 0.002 and C4 (men non-overweight: median: 23.40, mean rank: 16.60; men with risk of overweight/overweight: median: 26.40, mean rank: 26.36, p = 0.028; women non-overweight: median: 24.25, mean rank: 37.16 and women with risk of overweight/overweight: median: 32.80, mean rank: 54.42, p = 0.013. Conclusion: Inflammatory proteins are increased in adolescents with risk of overweight and overweight, particularly in women.Introducción: La inflamación de bajo grado y el aumento en la inmunidad relaciondas con las enfermedades cardiovasculares se han descrito en niños y adultos, sin embargo, estudios en adolescentes mexicanos se están haciendo en la actualidad. Objetivo: Evaluar las proteínas inflamatorias e indicadores de la inmunidad en los adolescentes por g

  11. 肝移植治疗Wilson病8例分析%Liver transplantation in treatment of Wilson's disease: a report of 8 cases

    Institute of Scientific and Technical Information of China (English)

    司马辉; 蔡文科; 邵卓; 杨宁; 杨广顺

    2011-01-01

    Objective To determine the efficacy and prognosis of liver transplantation for Wilson's disease (WD). Methods From October 2002 to August 2008, 8 cases (3 men and 5 women) with a mean age of 22.6 years (12 - 42 years) underwent liver transplantation for WD in our department. All the recipients had low serum levels of ceruloplasmin and copper before transplantation, and 5 of them demonstrated the neurological manifestations. Results There were 3 orthotopic liver transplantation (OLT) and 2 living donor liver transplantation (LDLT) in this study. Seven recipients recovered satisfactorily with excellent liver functions, while 1 patient died of acute liver steatosis in 1 month after transplantation. Discharged patients were well followed up for a mean duration of 79.7 months (35- 124 months). Compared to the levels before transplantation, the total bilirubin levels were significantly declined (P0.05). The neurological manifestations were also partly relieved or disappeared after liver transplantation. No K-P loop was found in the patients 12 months after operation. Conclusion OLT and LRLT are effective treatments for patients with WD, which not only improving their copper metabolism but also significantly relieving the severe neurological manifestations and improving life quality. (Shanghai Med J, 2011, 34: 656-659)%目的 探讨Wilson病患者接受肝移植治疗后的效果及预后.方法 回顾性分析2002年10月-2008年8月因Wilson病在第二军医大学附属东方肝胆外科医院接受肝移植手术的8例患者的临床资料,其中男3例,女5例,年龄为12~42岁,平均年龄为(22.6±10.3)岁.术前所有患者的血清铜蓝蛋白和血铜水平均低于正常值(血清铜蓝蛋白为200~600 mg/L,血铜为11~24.4μmol/L),其中5例术前存在神经病学症状.结果 5例患者接受原位肝移植术,3例接受活体肝移植术.除1例患者于术后1个月死于急性肝脂肪变肝功能衰竭外,其余患者术后肝功

  12. Biomarcadores del estado inflamatorio: nexo de unión con la obesidad y complicaciones asociadas Inflammatory biomarkers: the link between obesity and associated pathologies

    Directory of Open Access Journals (Sweden)

    M.ª A. Zulet

    2007-10-01

    humanos, así como los factores implicados en su regulación.The objetive of this article is to review biomarkers that have been suggested in recent years as the link between inflammation, obesity and associated co-morbidities, as well as some questions that yet remain unclear. Increasing evidence indicates the important role of inflammation in the etiology of major public health problems. In the last years, several studies have proposed that obesity might be a inflammatory disorder. In addition, oxidative stress has been suggested as a potential inductor of inflammatory status and susceptibility to obesity and related disorders. Several biomarkers are being suggested as the link between obesity, insulin resistance, cardiovascular disease and metabolic syndrome, such as tumor necrosis factor alfa, interleukin-6 and -18, angiotensinogen, transforming grow factor beta, plasminogen activator inhibitor-1, leptin, resistin, C-reactive protein, serum amyloid A, sialic acid, fibrinogen, markers of endothelial dysfunction (von Willebrand factor, ICAMs, VCAMs, complement factor 3, haptoglobin, Zinc-alpha2-glycoprotein, eotaxin, visfatin, apelin, alpha1- antitrypsin, vaspin, omentin, retinol binding protein 4, ceruloplasmin, adiponectin and desnutrin. Some of this biomarkers are good predictors of cardiovascular risk (plasminogen activator inhibitor-1, sialic acid, fribrinogen, complement factor 3, C-reactive protein, adiposity (leptin, visfatin, resistin, haptoglobin and/or insulin resistance (sialic acid, C-reactive protein, plasminogen activator inhibitor-1, von Willebrand factor. However, it is currently unclear the role of many of them concerning inflammatory processes in humans, as well as the factors involved in their regulation.

  13. The evaluation significance of ceruloplasmindetermination in multiple organ dysfunction syndrome (MODS) prognosis%血清铜蓝蛋白测定对MODS预后的评估意义

    Institute of Scientific and Technical Information of China (English)

    胡志华; 陈勉; 陈伟

    2012-01-01

    OBJECTIVE To explore the ceruloplasmin (CP) dynamic change in MODS patients to cause judgment and the value of the prognosis assessment. METHODS Determinedceruloplasmin, high sensitivity C-reactive protein (CRCP), α-acid glycoprotein (AAG) contents of 60 cases of MODS patients (MODS group) , 30 cases of patients with non-MODS (control group) when they were admitted into hospital, and contrasted in the MODS group, evaluated APACHE II and MODS score of all inpatients. RESULTS (1) There were differences in APACHE II, MODS score, CP, CRCP, AAG between MODS group and control group (P< 0.05); (2) comparison within MODS group: CP level was significantly different in patients with 3 and above organ damages and two organ damages (P< 0.05) , and there was no obvious relationship with liver damage. (3) CP and RCRP increased obviously in infection group and non-infection group (P< 0.05). CONCLUSION (1) CP, RCRP, AAG determinations helps early diagnosis in MODS. (2) With the number of organ damage increases, the CP levels increases to different extent, it is helpful to MODS prognostic diagnosis. (3) CP and RCRP monitoring helps preliminary judgment in causes of MODS, and has a certain value to specific therapy of MODS.%目的 探讨血清中铜蓝蛋白动态变化对MODS患者病因判断和预后评估的价值.方法 于入院当天分别检测60例MODS患者(MODS组)、30例非MODS患者(对照组)血清铜蓝蛋白(CP)、超敏C反应蛋白(CRCP)、α-酸性糖蛋白(AAG)乳酸的含量,并对MODS组进行组内比较,入选的住院患者均进行APACHEⅡ和MODS评分.结果 (1) MODS组与对照组相比其APACHEⅡ、MODS评分、血清铜蓝蛋白(CP)、超敏C反应蛋白(CRCP)、α-酸性糖蛋白(AAG)的比较,差异有统计学意义(P<0.05); (2) MODS组内比较:3个及3个以上器官损害与2个器官损害患者铜蓝蛋白水平比较,差异有统计学意义(P<0.05),且与是否有肝损害无明显关系; (3)非感染组与感染组相比较,铜

  14. Genetic characterization of a brangus-ibage cattle population: biochemical polymorphisms and reproductive efficiency Caracterização genética de uma população de bovinos brangus-ibagé: polimorfismos bioquímicos e eficiência reprodutiva

    Directory of Open Access Journals (Sweden)

    Luiz Ernani Henkes

    2000-10-01

    Full Text Available Biochemical techniques were used to investigate the genetic variability in a Brangus-Ibage population by determining allele frequencies of 18 blood protein systems: Hemogloin beta-Chain (Hb, Albumin (Alb, Amylase (Am, Transferrin (Tf, Carbonic Anhydrase (CA, Ceruloplasmin (Cp, Malic Enzyme (ME, Diaphorase I and II (Dia I and Dia II, Slow Alpha 2 Macroglobulin (Ap, Acid Phosphatase (ACP, Esterase B and D (EstB and EstD, Phosphogluconate Dehydrogenase (PGD, Glucose-6-Phosphate Dehydrogenase (G-6-PD, Glucose-Phosphate-Isomerase (GPI, Superoxide Dismutase (SOD and Glyoxalase I (GLO. The percentage of polymorphic loci were estimated at 0.27, the mean number of alleles was 1.33 and the mean heterozygosity was 0.07. There was a good agreement between expected and observed heterozygosity values. The population was in agreement with Hardy-Weinberg expectations in all systems. Reproductive records allowed to estimate three parameters of reproductive efficiency: mean age at first calving (1152.15 ± 166.60 days, mean calving interval (539.23 ± 124.10 days and mean weight at first calving (391.02 ± 37.59kg. No relationship was found between reproductive efficiency and genetic systems.Técnicas bioquímicas foram utilizadas para determinar a variabilidade genética numa população de bovinos da raça Brangus-Ibagé com relação a 18 sistemas protéicos sangüíneos: Hemoglobina - Cadeia beta (Hb, Albumina (Alb, Amilase (Am, Transferrina (Tf, Anidrase Carbônica (CA, Ceruloplasmina (Cp, Enzima Málica (ME, Diaforase I and II (Dia I and Dia II, Macroglobulina alfa2 lenta (Ap, Fosfatase Ácida (ACP, Esterase B and D (EstB and EstD, Fosfogliconato Desidrogenase (PGD, Glicose-6-Fosfato Desidrogenase (G-6-PD, Glicose-Fosfato-Isomerase (GPI, Superóxido Dismutase (SOD e Glioxalase I (GLO. O percentual de locos polimórficos foi estimado em 0,27, o número médio de alelos foi 1,33 e a heterozigosidade média foi de 0,07. Houve boa concordância entre a

  15. Health effects following long-term exposure to thorium dusts. A fourteen-year follow-up study in China

    International Nuclear Information System (INIS)

    A fourteen-year follow-up study was carried out at Baiyan Obo Rare-earth Iron Mine in China. It has been operating for forty one years. It's ore contains ThO2 at a percentage of 0.04. The purpose of this study is to investigate the possible health effects of 2903 dust-inhaled miners following long-term exposure to thorium dusts. An electrostatic collection system was used to measure the exhaled thoron activity of each examinee, through simple calculation, each examinee's thorium lung burden obtained. Health status was ascertained through questionnaire, physical examination and clinical laboratory tests. An epidemiological study on the mortality rates of lung cancers of the dust-inhaled miners and internal controls was also carried out. Results showed that the highest thorium lung burden for 1158 measurements of 638 miners was at a value of 11.11 Bq, one tenth of the maximum thorium lung burden. In a ten-year investigation on the four haematological parameters and four hepatic parameters of 638 exposed miners, no adverse effects were observed. The incidence of severe breathlessness, lung function tests, pneumoconiosis of stage O+ and the concentration of ceruloplasmin in the serum of high thorium lung burden group are much higher than that of low thorium lung burden group. However, owing to the concentration of SiO2 (10%) are much higher than that of the ThO2 (0.04%) in the dust, the disorders of respiratory tract are mainly due to the fibrogenic effects of SiO2, Epidemiology study showed that both the SMRs of the dust-inhaled miners and the dust-free miners are all greater than one (5.15 vs. 2.30). The SMR of the dust miners was much higher than that of the controls. However, there was no statistic significant difference between these two SMRs (X2=3.75 P>0.05). This means that the high SMR of the dust-inhaled miners was not solely due to the long-term inhaled thorium and short-lived thoron daughters. Because of very high percentage of smokers (- 80%) among both the

  16. The malarial impact on the nutritional status of Amazonian adult subjects Impacto da malaria no estado nutricional de doentes adultos da Amazônia

    Directory of Open Access Journals (Sweden)

    Paulo C. M. Pereira

    1995-02-01

    Full Text Available The anthropometric (body weight, height, upper arm circumference, triceps and subescapular skinfolds; Quetelet index and arm muscle circunference and blood biochemistry (proteins and lipids parameters were evaluated in 93 males and 27 females, 17-72 years old voluntaries living in the malarial endemic area of Humaita city (southwest Amazon. According to their malarial history they were assembled in four different groups: G1-controls without malarial history (n:30; G2 - controls with malarial history but without actual manifestation of the disease (n:40; G3 - patients with Plasmodium vivax (n:19 and G4 - patients with Plasmodium falciparum (n:31. The malarial status was stablished by clinical and laboratory findings. The overall data of anthropometry and blood biochemistry discriminated the groups differently. The anthropometric data were low sensitive and contrasted only the two extremes (G1>G4 whereas the biochemistry differentiated two big groups, the healthy (G1+G2 and the patients (G3+G4. The nutritional status of the P. falciparum patients was highly depressed for most of the studied indices but none was sensitive enough to differentiate this group from the P. vivax group (G3. On the other hand the two healthy groups could be differentiated through the levels of ceruloplasmin (G1G2. Thus it seems that the malaria-malnourishment state exists and the results could be framed either as a consequence of nutrient sink and/or the infection stress both motivated by the parasite.A avaliação antropométrica (pêso, altura, circunferência branquial, prega cutânea tricipital, prega cutânea subescapular, índice de Quetelet e circunferência muscular do braço e bioquímica (proteínas e lipides foi realizado em 120 indivíduos (93 masculinos e 27 do sexo feminino, de 17 a 72 anos de idade, moradores de área endêmica de malária (Humaitá -AM. De acordo com a história da doença (malária eles foram divididos em 4 grupos: G1 - controle (n = 30

  17. Quadro seroproteico como auxílio diagnóstico na anemia hemolítica imunomediada em cães Serum proteic profile as diagnosis aids in immune-mediated hemolytic anemia in dogs

    Directory of Open Access Journals (Sweden)

    Patrícia Mendes Pereira

    2010-04-01

    maior nestes. Tais achados analisados em conjunto agregam informações adicionais úteis à elucidação das AHIMs em cães.This assay aimed to determine the serum protein - via polyacrylamide gel electrophoresis, which contained duodecil sodium sulfate (SDS-PAGE - in 120 dogs, with different breeds and ages, seen by the Veterinary Hospital "Governor Laudo Natel." These animals were grouped into five experimental groups: Group 1 - group control with 20 dogs, group 2 - 28 dogs with regenerative anemia; group 3 - 27 dogs with arregenarative; anemia group 4 - 10 dogs with primary immune-mediated hemolytic anemia (AHIM 1.rd; group 5 - 35 dogs with secondary immune-mediated hemolytic anemia (AHIM 2.rd. The technique allowed the SDS-PAGE fractionation of 24 protein, whose molecular weights (PM ranged from 18,000 to 165,000 daltons (Da. The dogs with 1st and 2nd AHIM showed 24 protein fractions in their tracks electrophoretic, while other groups of dogs showed 23 fractions of protein, whose molecular protein weight of 68,000Da was not found. Thus, twenty-three proteins were common to proteinograms of the five experimental groups. From these, it was possible to identify eleven protein fractions nominally, and others were identified by their molecular weights. For control dogs, the anemic (groups 2, 3, 4 and 5 showed higher concentrations of serum transferrin and between them, the animals carrying the primary IMHA. All groups of dogs showed anemic levels of serum haptoglobin and phosphorylase significantly higher than the control dogs, while the serum ceruloplasmin was lower in anemic dogs. These findings provide additional information to the elucidation of the AHIMs in dogs.

  18. Endothelial cells the site of the regulation of IgG LEVEL by salvaging and transcytosis of immunoglobulin

    International Nuclear Information System (INIS)

    ), transferrin (Tavassoli et al 1986), ceruloplasmin (Tarsio et al 1987), transcobalamin II (Soda et al 1985) the complex biochemical events taking place at the blood-vessel wall interface are not fully understood. This is the case of IgG transfer from mother to fetus. The process is thought to involve specific receptors that bind to the Fc region of the IgG molecule namely the MHC class I related receptor FcRn (Brambel 1970, Rodewald 1980). Until now, the reported data showed that FcRn was occasional (Kristofersen et al 1996) low level (Leach et al 1996) or not at all expressed (Simister et al 1996) in fetal endothelial cells. Taking advantage of the successful isolation and cultivation of microvascular endothelial cells from human term placenta (Jinga V et al 1999) we can now appropriately investigate the presence, distribution and function of FcRn in placental endothelial cells.Transfer of maternal gammaglobulin (IgG) to the fetus provides the neonate with humoral immunity during early lifetime. The mechanism of selective transport of IgG from the placental stroma to the lumen of the fetal blood vessels has not been elucidated, yet. It was postulated that the specific transport as well as the regulation of IgG concentration in the blood, involves the MHC class I related receptor - FcRn - for the Fc domain of IgG. We questioned whether, human placental endothelial cells (HPEC) express FcRn, and if present, whether it is in a functionally active form. The experiments were performed on cultured HPEC; as positive control, cultured human trophoblastic cells (JEG3) and mouse SV-40 transformed EC line (SVEC) shown to express mRNA for the human FcRn, were used. The double chamber system. The permeability coefficient expression of FcRn was tested by indirect immunofluorescence. The role of FcRn was assessed on HPEC grown on filters in a double chamber system by quantifying the transcytosis of [125 I]-human IgG or [125 I]-rF(ab')2 fragments (as control) from the apical to basolateral

  19. Electrophoresis of Serum Proteins in Patients with Hepatitis B Virus - associated Hepatocellular Carcinoma%HBSAg阳性原发性肝癌患者血清蛋白电泳图谱分析

    Institute of Scientific and Technical Information of China (English)

    高国生; 徐晓珍

    2012-01-01

    . Meantime, serum albumin (ALB),α1-antitrypsin(AAT), al-phafetoprotein (AFP), haptoglobin(Hp), Ceruloplasmin (Gp),transferrin(TRF), complement C3(C3), immu no globulin G(IgG), immu-noglobulin A(IgA),immunoglobulin M(IgM) and C-reactive protein(CRP) were detected. Then a statistical analysis was made on these results. Results In all groups,the percent of albumin in patients with hepatitis B virus-associated hepatocellular carcinoma was lowest, and the difference compared with healthy adults and patients with chronic hepatitis B had statistical significant (P<0.05). The percent of α1-globin was highest, and the difference compared with healthy adults and patients with posthepatitic cirrhosis had statistical significance(P<0.05), The percent of α2-globin was higher than other patients. And the level of serum AAT,AFP, Cp and CRP was significantly higher than that in other groups (P<0.05). The level of serum Hp and C3 was significantly higher than that in other patients (just a little lower than that in healthy adults).Conclusion The changes of serum protein electrophoresis in patients with hepatitis B virus-associated hepatocellular carcinoma were mainly presented with the decreased level in albumin and increased percent of α1 and α2-glo-bins. Increased percent of α-globins markedly related with increased positive acute phase proteins and AFP. Serum protein electrophoresis combined with detection of specific proteins was important for the diagnosis of disease and the assessment of disease progression.

  20. Neurodegeneration with Brain Iron Accumulation: An Overview

    Directory of Open Access Journals (Sweden)

    Seyed Hassan TONEKABONI*

    2014-12-01

    . Absence of an orphan mitochondrial protein, c19orf12 with brain iron accumulation, Am J Hum Genet 2011;89: 543-550.Najim al-Din AS, Wriekat A, Mubaidin A, et al. Pallidopyramidal degeneration, supraneuclearupgaze paresis and dementia: Kufor- Rakeb syndrome. Acta Neurol Scand 2011;89: 347-352.Tobias B Hoak, Penelope Hogarth, Micheal C Kruer et al. Am J Hum Genet 2012 Dec 7; 91 (6: 1144-49.Chummery PF, Crompton DE, Bircholl D, et al. Clinical features and matural history of Neuroferritinopathy caused by the FTL1 gene mutation. Brain 2007;130:110-119.Mc Neil A, Bircholl D, Hayflich SJ, et al. T2 and FSE MRI distinguishes L subtypes of NBIA, Neurology 2008;70: 1614- 1619. McNeil A , Pandolfo M, Kuhn J,et al.The Neurological presentation of ceruloplasmin gene  mutations. Eur Neurol 2008;60:200-205.Dusi S, Valletta L, Hoach TB, et al. Exone sequencing reveals mutations in Co A synthtas as a cause of neurodegeneration with brain iron accumulation: Am J Hum Genetic Jan2, 2014. Aras M Alazim, Amir Alsaif, Abdulaziz Al-Semari, et al. mutation in C2 orf 37, cause hypogonadism, diabetes Melitus, Mental retardation and extrapyramidal syndrome: Am J Hum Genetic. 2008 Dec 12; 83(6: 684-691.

  1. Citrin缺陷所致婴儿肝内胆汁淤积症43例分析%Intrahepatic cholestasis caused by citrin deficiency:a clinical analysis of 43 infants

    Institute of Scientific and Technical Information of China (English)

    姜涛; 姚蔚; 欧阳文献; 谭艳芳; 李双杰

    2014-01-01

    Objective To analyze the clinical characteristics and prognosis of neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD)in infants.Methods The clinical data of 43 infants with NICCD in our hospital from July 201 1 to April 2014 were collected.The diagnosis was confirmed by tandem mass spectrometry (MS-MS)analysis of blood,gas chromatography-mass spectrometry (GC-MS)a-nalysis of urine,and genetic testing,and an analysis was performed with reference to clinical manifestations and laboratory results.Results Most patients with NICCD developed jaundice early,with a round,fat face,hepatomegaly,and growth retardation.Laboratory examina-tions showed increased bilirubin,total bile acids,blood lactic acid,alpha-fetoprotein,and procalcitonin in all patients,increased alanine aminotransferase,gamma-glutamyl transpeptidase,and blood ammonia,decreased albumin and blood glucose,and dyslipidemia and coag-ulation disorders in most patients,and abnormal liver fibrosis markers (20/24)and low ceruloplasmin (17/20)in some patients.Abdomi-nal ultrasound showed hepatomegaly in 32 patients.MS-MS analysis of blood samples revealed distinctive elevation of methionine,citrul-line,tyrosine,threonine,and a variety of acyl carnitine in most patients.GC-MS analysis of urine samples revealed elevated galactose and galactitol in 20 patients,elevated 4-hydroxyphenyllactic acid and 4-hydroxyphenylpyruvic acid in 10 patients,and no abnormalities in 6 patients.Genetic testing revealed 851del4,1638ins23,and IVS6+5G>A mutations,especially 851del4 mutation.After being treated by giving lactose-free diet,strengthening medium-chain fatty acid diet,protecting the liver,and relieving cholestasis,most patients had nor-malized indices 2 months later,2 cases had liver cirrhosis,and 7 cases died.Conclusion The clinical manifestations of NICCD vary in children.MS-MS analysis of blood,GC-MS analysis of urine,and genetic testing should be performed early in children with clinically suspected NICCD

  2. Mutation and Copy Number Variation Analysis of ATP7A Gene in Patients with Menkes Disease%Menkes病临床及ATP7A基因突变和拷贝数改变分析

    Institute of Scientific and Technical Information of China (English)

    黄琼辉; 王静敏; 吴晔; 邓艳华; 熊晖; 张月华; 杨艳玲; 姜玉武

    2012-01-01

    Objective To analyze ATP7A gene mutations and copy number variation( CNV) in 13 Chinese patients with classical Menkes disease (MD) and discuss the relationship of genotype and phenotype. Methods Thirteen patients clinically diagnosed Menkes disease were included. All 23 exons and exon - intron boundaries of ATP7A gene were amplified by polymerase chain reaction ( PCR) and directly se-quenced for genomic DNA. The mutation had been proved by DNA restriction enzyme digestion of PCR - amplified fragments. Multiplex liga-tion - dependent probe amplification( MLPA) assays were performed to detect ATP7 A copy number variation. Results All of 13 patients were boys. The symptoms onset occurred between 3 days to 7 months. The manifestations were typical hair changes, seizures, hypotonia, neurodevelo-pmental delays and inguinal hernias. Twelve cases(100% ,n = 12)patients had low serum ceruloplasmin concentration;6 cases(75% ,n = 8) patients had low serum copper concentration;4 cases ( 100% , n = 4) patients had " corkscrew" appearance of cerebral vessels in MR angiography. Five patients had been found dead before 14 months old during follow - up. One patient was 47XXY/46XY chimerism;Six mutations were identified in 8 of 13 patients through DNA sequencing,including 2 missense mutations ( c. 3028A > C,c. 2179G > A) ;2 frame - shift mutations (one complicated deletion mutation:c. 2589delTGAAGGA + c. 2598delTT + c.2601delT + c. 2603delT + c. 2605delGTAGATGA, one simple deletion:c. 3045delT) and 2 splicing mutations (e.4005 +1G >C,c. 3658 + 1G > A); three of these were novel. MLPA assays identified one case with deletion covered whole exon 10 which was proved by long -fragment PCR as c.2173 -346_2407 -346del,p. F725_ K802 del. With the same mutations c. 2179G > A( p. G727R) ,P1 which was 47XXY/46XY,had earlier onset and heavier clinical symptoms than P2 which was the normal karyotype. Conclusions MLPA is a rapid and reliable technique to detect the big fragment or whole gene

  3. Radicais livres de oxigênio e exercício: mecanismos de formação e adaptação ao treinamento físico Radicales libres de oxigeno y ejercicio: mecanismos de formación y adaptación al entrenamiento Oxygen free radicals and exercise: mechanisms of synthesis and adaptation to the physical training

    Directory of Open Access Journals (Sweden)

    Cláudia Dornelles Schneider

    2004-08-01

    formación de los RLO y de adaptación al estrés oxidativo crónico provocado por el entrenamiento físico.The interest about the mechanisms of generation and adaptation of oxygen free radicals (OFR to exercise has increased significantly from the demonstration of its relation with the oxygen intake. The OFR are formed through the incomplete reduction of oxygen, generating species presenting high reactivity to other biomolecules, especially lipids and proteins of the cell membranes and even DNA. The injuries caused by the oxidative stress present accumulative effects, being related to several diseases such as cancer, arteriosclerosis and diabetes. The acute physical exercise furthers the increase on the formation of OFR in function of the increment on the oxygen intake. However, the physical training generates adaptations able to soften the harmful effects caused by OFR. These adaptations are related to several systems, among which the most important are the enzymatic system, composed by the superoxide dysmutase, catalase and glutathione peroxidase; and the non-enzymatic system, composed by the ceruloplasmine, the sexual hormones, co-enzyme Q, uric acid, thermal shock proteins, among others. Such adaptations, despite the controversies about the mechanisms involved, further a higher tissue resistance and oxidative challenges such as those provided by long-duration high intensity exercises. The evaluations techniques of the oxidative stress, most times are not able to detect injuries in short-duration exercises. Thus, studies of physical efforts performed for long periods or until exhaustion have been conducted. New lesion markers by OFR action have been discovered and new techniques for its determination have been created. The objective of this work is discuss the formation mechanisms of OFR and the adaptations to the chronic oxidative stress caused by physical training.