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Sample records for celulas hibridas dendriticas

  1. BUDAYA HIBRIDA MASYARAKAT CIREBON

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    M. Alie Humaedi

    2013-10-01

    Full Text Available Cirebon dikenal salah satu simpul utama jejaring distribusi ekonomi di Nusantara. Kota ini juga telah menciptakan jejaring kebudayaannya sendiri sehingga berbagai fenomena kebudayaan beserta transformasi sosialnya seringkali muncul dalam bentuk yang berbeda dengan pusat-pusat kebudayaan mainstream, baik kebudayaan (dan bahasa Sunda ataupun Jawa. Pertanyaannya, bagaimana jejaring kebudayaan hibrida terbentuk seturut pertumbuhan kota? Bagaimana hibriditas kebudayaan dipraktikkan masyarakat ketika terjadi proses silang budaya sebagai warisan genealogis kebudayaannya?. Studi yang menggunakan pendekatan etnografis melalui wawancara mendalam dan pengamatan langsung ini hendak menjelaskan bagaimana masyarakat Cirebon mengartikulasikan pandangan mereka tentang kotanya dan memetakan hubungan entitas etnik di antara mereka, yaitu antara orang Sunda sebagai pemilik kebudayaan mainstream dan orang Jawa Cirebon (wong Jawa Cerbonan-Jawa Koek sebagai pemegang kebudayaan marjinal dalam membangun ruang kehidupannya. Penelitian menemukan bahwa proses pertemuan silang budaya telah menghasilkan hibriditas kebudayaan yang tidak pernah jelas batas-batasnya. Bahkan, dapat melahirkan pola kebudayaan baru yang sifatnya lebih condong kepada kebudayaan mainstream, atau juga pada kebudayaan marginal sebagaimana terlihat pada kasus perkawinan silang budaya yang melahirkan pilihan penggunaan bahasa, yaitu Sunda Koek atau Jawa Koek yang berbeda dengan pusat kebudayaannya masing-masing.

  2. Sifat Anatomi dan Fisis Kelapa Hibrida

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    Hutabarat, Marihot Hamonangan

    2010-01-01

    Penelitian ini bertujuan untuk mengevaluasi sifat anatomis dan fisis batang kelapa hibrida. Ciri anatomi diamati langsung pada sampel dan menggunakan mikroskop untuk pengamatan dimensi seratnya, dan pengujian sifat fisis menggunakan British Standard. Sifat anatomis dan fisis batang kelapa hibrida bervariasi menurut ketinggian dan kedalaman. Struktur anatomis batang kelapa hibrida didominasi oleh vascular bundle dan parenkim. Serat kayu dapat ditemukan didalam vascular bundle dengan rata-r...

  3. Efecto de la inhalacion del pentoxido de vanadio (V2o5) en la morfofisiologia de las celulas dendriticas (CD) del timo de raton /

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    Ustarroz Cano, Martha Luz

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Biológicas, presenta Martha Luz Ustarroz Cano ; asesor Teresa Imelda Fortoul van der Goes62 páginas : ilustracionesDoctorado en Ciencias Biológicas UNAM, Facultad de Medicina, 2012

  4. KARAKTERISTIK FENOTIPE HIBRIDA HUNA BIRU (Cherax albertisii DENGAN HUNA CAPITMERAH (Cherax quadricarinatus

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    Irin Iriana Kusmini

    2016-11-01

    Full Text Available Lobster air tawar (huna adalah spesies endemik dan merupakan komoditas perikanan spesifik lokal Papua dan Australia, yang termasuk famili Parastacidae dan genus Cherax. Secara morfologi ada persamaan bentuk dan warna antara huna biru dengan capitmerah. Diduga terjadi inbreeding yang menyebabkan produksi huna capitmerah mulai menurun. Hibridisasi antar huna bertujuan untuk meningkatkan keragaman genetiknya sehingga diperoleh kualitas benih huna yang lebih baik dalam hal pertumbuhan dan sintasan. Hasil penelitian menunjukkan bahwa pertumbuhan turunan hibrida antara huna capitmerah dengan huna biru sampai 4 bulan relatif sama dengan non hibrida, pada umur 5 bulan pertumbuhan hibrida mulai terlihat lebih cepat dibandingkan dengan yang non hibrida. Hibridisasi jantan huna biru dengan betina huna capit merah (AR menghasilkan hibrida dengan efek heterosis 25% pada kenaikan bobot badannya. Hibridisasi jantan huna capitmerah dengan betina huna biru (RA menghasilkan nilai sintasan yang lebih baik dibandingkan dengan AR

  5. Hybrid membranes PVA/silicon for use in fuel cells; Membranas hibridas de PVA/silica para aplicacao em celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Aguiar, Liz C.V. de; Almeida, Raquel D. de; Gomes, Ailton de S. [Universidade Federal do Rio de Janeiro - UFRJ, Instituto de Macromoleculas Professora Eloisa Mano - IMA, RJ (Brazil)], e-mail: lizcontino@ima.ufrj.br; Ramos Filho, Florencio G. de [Centro Universitario Estadual da Zona Oeste - UEZO, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    Hybrids organic-inorganic membranes PVA-silica have been prepared using sol-gel process in situ with the objective of study the influence of inorganic particles incorporation on the water uptake, pervaporation and proton conductivity of PVA membranes. The silica was constituted of mercaptopropyltrimethoxysilane (MPTMS) with or without the mixture with the tetra ethoxysilane (TEOS). The hybrids membranes were oxidated to convert the -SH groups in -SO{sub 3}H groups, becoming them proton conducting. The hybrids membranes not oxidated showed lesser water uptake and pervaporated material, probably, due to the formation of crosslink that restricted the swell of the PVA membrane. The protonic conductivity of the hybrid membranes after the oxidation was up to 26 times bigger than of the membrane not oxidated. (author)

  6. MODELO PARA UNA RED HIBRIDA ÓPTICA INALÁMBRICA

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    Luis Alejandro Quiroz

    2012-08-01

    Full Text Available Este artículo propone un modelo de red hibrida óptica - inalámbrica mediante la tecnología free space optics (FSO para el desarrollo de una red de acceso de nueva generación, que represente una solución a los proveedores para diferenciación de servicios, ofrecimiento de ancho de banda bajo demanda y uso compartido de infraestructura con otros  proveedores de servicios. Se describe el principio de funcionamiento y las características técnicas de la tecnología FSO, se expone y analiza la arquitectura y el algoritmo de reconfiguración  propuesto.

  7. Hibridação de Verdades Políticas e Midiáticas

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    Maria Helena Weber

    2008-12-01

    Full Text Available O artigo aborda questões vinculadas ao exercício de relações e poderes, entre mídias e política, quando em disputa pela posse da verdade, autoridade, credibilidade e opiniões. Para tanto analisa um texto-espetáculo (campanha publicitária denominado A vida por todos os lados, do jornal Zero Hora, através da aplicação da Estrutura de Hibridação Mediática, cujas estratégias sustentam as mídias. Essa campanha é exemplar no cenário das relações e discursos específicos que demarcaram as ações do ano de 1999 entre dois sujeitos poderosos, o Governo do Estado do RGS, administrado pelo PT e a Rede Brasil Sul de Comunicações, tendo mobilizado disputas em espaços políticos, acadêmicos e institucionais

  8. Melhoramento do cafeeiro: V - Melhoramento por hibridação

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    C. A. Krug

    1952-06-01

    Full Text Available A hibridação como método de melhoramento do cafeeiro, oferece amplas possibilidades que ainda se acham pouco exploradas. Para o caso da espécie C. arabica, a hibridação entre suas variedades, além de fornecer dados seguros sôbre a constituição genética, permite também a verificação de ocorrência da heterose, o melhoramento sem mudança dos caracteres das variedades, ou sintetização de estruturas genéticas novas. Os cruzamentos interespecíficos poderão contribuir decisivamente para a solução do problema da melhoria da qualidade do produto e dar indicações a respeito das relações das várias espécies e da reação dos fatôres genéticos de uma espécie em ambientes genéticos diversos. Cêrca de 2500 híbridos se acham em estudo na Secção de Genética, envolvendo plantas da mesma variedade, plantas de variedades diferentes e cafeeiros pertencentes a espécies distintas. As diversas possibilidades dêsses cruzamentos no melhoramento do cafeeiro são indicadas, chamando-se especial atenção para os híbridos interespecíficos, principalmente aquêles que envolvem a espécie tetraplóide C. arabica e outras espécies diplóides, como C. canephora, C. Dewevrei e C. congensis. Êstes híbridos triplóides, após duplicação do número de cromosômios, poderão constituir fonte de novas formas de Coffea, permitindo a expansão do cultivo do café era São Paulo.Artificial hybridization in coffee breeding offers possibilities that are still unexplored. Inter-varietal hybridization has been used in Coffea arabica in order to get information about the genetic constitution of the varieties, and occurrence of heterosis, to breed without changing the morphological characters of the varieties and also to synthesize new genetic types of economic value. Inter-specific hybridization has been employed for improvement of cup quality and to furnish data about the relationship of various coffee species and the behaviour of known

  9. San Juanico, BCS, Mexico, hybrid electric plant renewable energies in the rural communities development; Planta electrica hibrida San Juanico, B.C.S., Mexico, las energias renovables en el desarrollo de las comunidades rurales

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    Lopez Rios, Serafin [Gerencia de Proyectos Geotermoelectricos, Comision Federal de Electricidad (Mexico)

    1999-08-01

    The hybrid electric plant of San Juanico, B.C.S., Mexico, is described in terms of its environmental goals, operating process and contribution to the development of that rural community of the Baja California Peninsula. San Juanico hybrid electric plant is organized in three electrical generation systems that work in parallel: one uses solar energy, another wind energy and a third one uses diesel fuel. [Spanish] Se describe la planta hibrida de San Juanico, BCS, Mexico, en terminos de los objetivos ambientales que condujeron a su realizacion, asi como de su proceso operativo y de la participacion que tiene en el desarrollo de esa comunidad rural de la peninsula de Baja California, Mexico. La planta hibrida de San Juanico esta constituida por tres sistemas de generacion de electrcicidad que operan en paralelo: uno utiliza energia radiante del sol, otro energia del viento y un tercero utiliza diesel.

  10. Produção de híbridos de amendoim forrageiro por meio de hibridação artificial Production of forage peanut hybrids through artificial hybridization

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    Marilda Augusta Peres Oliveira

    2002-06-01

    Full Text Available O objetivo deste trabalho foi a obtenção de híbridos de amendoim forrageiro por meio da hibridação artificial. O experimento foi realizado na Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, durante a época de florescimento dos acessos de Arachis pintoi Krap. & W. C. Gregory e de A. repens Handro. Cerca de 700 polinizações produziram 27 segmentos de frutos, com taxas de fecundação que variaram entre 1,1 e 12,9%, considerando-se todas as combinações híbridas. Os híbridos intra-específicos de A. pintoi produziram sementes F2, e os interespecíficos não produziram semente. A técnica de hibridação utilizada nas espécies forrageiras necessitou de ajustes, devido a diferenças observadas em relação ao amendoim cultivado, entre elas o hábito de crescimento.The purpose of this work was to obtain forage peanut hybrids through artificial hybridization. The experiment was conducted in a screenhouse at Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia during the flowering period of Arachis pintoi Krap. & W. C. Gregory and A. repens Handro accessions. About 700 pollinations produced 27 fruit segments and the fertilization rates ranged from 1.1 to 12.9% for all cross combinations. The intraspecific hybrids produced F2 seeds, which did not occur to the interspecific hybrids. To effect the hybridization technique, adjustments were necessary to forage Arachis species, in relation to cultivated peanut, since differences in the growth habit were verified.

  11. Studi Pemanfaatan Catu Daya Hibrida PLTS 3,7 kWp Dan PLN Pada Instalasi Pengolahan Air Limbah Desa Pemecutan Kaja Denpasar Bali

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    Aries Arimbawa

    2016-11-01

    Full Text Available Abstract—Pemecutan Kaja waste treatment plant (WTP was a community treatment plant located in one of Denpasar city ward to process sewerage produced by community members. The WTP uses radial flow anaerobic system which mainly consists of rabic pro and up flow tanks. The water produced by the WTP was released to nearby river as it already met envinronmental quality standard. The WTP was driven by an electric pump to circulate sewer material within the process flow. The pump was fed by hybrid power supply combining 3.7 kW solar PV and power from utility grid. The WTP was operated by village council of Pemecutan Kaja. The study presented here was result of firstly, evaluation on the utilization and performance of solar PV plant, and secondly, to propose managerial model that can manage the plant effectively and sustainably. The study found that daily average energy produced bythe PVplant was 23.59 kWh yielding cost of energy at IDR 7,766/kWh. Experiment to clean filters of the plant reduced daily energy consumption from 8.84 kWh to 3.05 kWh or 65%. Household that connected to the plant pay monthly subscription currently at IDR 10,000.  However, for sustainable operation of the plant, the household need to pay IDR 51,654. Keywords: Renewable Energy, Solar PV, Hybrid Power Supply, Waste Treatment, Managerial Model Abstrak—Instalasi Pengolahan Air Limbah (IPAL Desa Pemecutan Kaja adalah IPAL yang terletak di salah satu lingkungan kota Denpasar yang berfungi untuk mengolah air limbah yang dihasilkan oleh anggota masyarakat. IPAL ini meggunakan sistem radial flow anaerobic yang terdiri tangki rabic pro dan tangki up flow filter. Air hasil pengolahan limbah dapat langsung disalurkan ke sungai karena sudah memenuhi baku mutu limbah cair. IPAL ini menggunakan pompa listrik untuk mengalirkan limbah menuju tangki penyaringan. Pompa ini mengunakan catu daya hibrida PLTS 3,7 kW dan PLN. IPAL ini dikelola langsung oleh masyarakat desa Pemecutan Kaja. Hasil

  12. Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization Ocorrência de Listeria monocytogenes em silagens avaliadas por meio da hibridação in situ fluorescente

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    M. Oliveira

    2008-02-01

    Full Text Available Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%. Por meio do protocolo FISH, observou-se que 22 silos (29,7% se encontravam contaminados. O método FISH apresentou precisão (77,0%, especificidade (92,5% e sensibilidade (72,7% elevadas, sendo adequado para a análise microbiológica presuntiva de silagens, uma vez que é um método rápido, fácl de realizar, sensível, reprodutível e pouco dispendioso.

  13. Biologia floral, sistema reprodutivo e métodos artificiais de hibridação de Hemerocallis hybrida Floral biology, reproductive system and artificial methods of hybridization of Hemerocallis hybrida

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    Sara Pereira Menezes

    2011-02-01

    Full Text Available A espécie ornamental Hemerocallis hybrida Hort. é uma planta herbácea perene originária da Ásia. Conduziu-se este trabalho, com o objetivo de estudar métodos artificiais de hibridação, biologia floral e o sistema reprodutivo de plantas de 'lírio-de-São-José', com vistas a gerar subsídios a programas de melhoramento genético dessa espécie ornamental. A técnica de cobertura do estigma de flores de 'lírio-de-São-José', ao invés de se cobrir toda a flor, após a realização do cruzamento artificial, proporciona maior taxa de pegamento e maturação de cápsulas de sementes (p The ornamental species Hemerocallis hybrida Hort. is a perennial herbaceous plant originated in Asia. The objective of the present work to study the artificial methods of hybridization¸ floral biology and reproductive system of daylily plants to support genetic and breeding programs of this species. The technique of covering the stigma of daylily's flowers with aluminum paper instead of covering the whole flower after the artificial crossing, provides higher setting and maturation of seed capsules (p < 0,001; Test ² contingency 2 x 2; correction of Yates. Blooming is the best phenophase for controlled crossings with estigmatic reception of 100%, and amount of viable pollen grains of 45% higher then the phase of the senescence. The studied species is hermaphrodite, essentially allogamous, with self-incompatibility and autogamy indexes equal to zero.

  14. Técnica quirúrgica hibrida de fijación externa descartable (fed y prótesis articulada en la en la corrección de subluxacion tarso crural.

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    Francisco Cavero Alprecht, Manuel Cardenas Rodríguez, Fernando García Bracamonte.

    2006-09-01

    Full Text Available La subluxación tarsocrural en caninos esfrecuente en la practica clínica, seproduce por torceduras o hiperextensiónde la articulación, acompañándose con laruptura o desgarro de uno o ambosligamentos laterales, de la capsulaarticular y en ocasiones con fracturas delastrágalo o uno o ambos maléolos de latibia. La reducción puede ser abierta ocerrada. Las técnicas tradicionales deelección para resolver esta patologíadificultan la recuperación debido altiempo de inmovilización de laarticulación además necesitan unprolongado periodo de rehabilitación,obteniéndose un éxito relativo. Sepresento un canino macho de razaindefinida de 5 meses de edad con unpeso de 10 Kg. Al examen clínico yradiológico se diagnosticó subluxacióntarso crural en el miembro posteriorizquierdo y fractura de tibia del miembroposterior derecho.En la corrección quirúrgica del miembroposterior izquierdo se desarrollo unatécnica hibrida: Fijación esqueléticaexterna descartable (FEED con unabarra stabilizadora de metil metacrilatounida a una prótesis articulada usadapara evitar la interferencia de la fibrosisen la biomecánica articular. Comoresultado se obtuvo temprana reposiciónde la funcionalidad normal sincomplicaciones post-acto quirúrgico. Lamovilización temprana otorgada por laprótesis articulada permitió un normalfuncionamiento de la articulación y unarápida recuperación, evitandoprolongados periodos de rehabilitaciónluego del retiro de la FEED 4 semanasdespués de la cirugía.SummaryThe Tarso-Crural subluxation in dogs isfrequently seen in the daily practice. Thispathology is produced when a sprain orhyperextension of the joint takes place,together with the rupture or tear, of oneor both lateral ligaments and the jointcapsule. Sometimes, this can alsoproduce a fracture of the astragalus orone or both maleolus of the tibia. Thereduction of this kind of fracture can bedone in close or open techniques. Thetraditional technique, usually

  15. Caracterização citogenética, viabilidade de pólen e hibridação artificial em gérbera Chromosome number, pollen viability and gerbera hybridization

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    Raquel DL Cardoso

    2009-03-01

    Full Text Available Este trabalho foi conduzido com o objetivo de confirmar o número de cromossomos em cultivares de Gerbera hybrida Hort., determinar o número de cromossomos em acessos não comerciais de Gerbera sp., avaliar a viabilidade de pólen e a possibilidade de cruzamentos entre cultivares e acessos não comerciais. Foram coletados ápices de raízes e pólen de seis cultivares e de sete acessos não comerciais. O material coletado foi corado com carmim acético a 45%. A contagem dos cromossomos foi realizada em células metafásicas intactas e a estimativa de viabilidade de pólen realizada por meio da contagem do número de grãos de pólen viáveis e não viáveis. A possibilidade de cruzamento entre as cultivares e entre as cultivares e acessos não comerciais foi avaliada por meio da hibridação entre os genitores femininos, cv. Terra Fame e acesso A8, e masculinos, cvs. Cariba e Azteca. Todos os acessos contiveram cinqüenta cromossomos, indicando que a variação morfológica nos capítulos (simples, semidobrado e dobrado não é devida a mutações cromossômicas numéricas ou a poliploidia. A viabilidade do pólen variou de 87,67% a 99,27%. A formação de sementes foi de 4,46% nos cruzamentos entre cultivares, e de 50% entre o A8 e as cultivares. A compatibilidade genômica entre os acessos, a alta viabilidade do pólen e o sucesso na obtenção de sementes entre acessos comercias e não comerciais, revela a possibilidade de produção de híbridos com novas combinações alélicas e transferência de caracteres desejáveis dos acessos não comerciais para os comerciaisThis work was conducted to confirm the chromosomes number of Gerbera hybrida Hort. cultivars, to determine the chromosomes number in the non commercial accessions of Gerbera sp., and to estimate the pollen viability and the possibility of crossings among different accessions. Root-tip and pollen were collected from six cultivars and seven non commercial accessions. The collected

  16. Melhoramento genético da cana-de-açúcar: avaliação de clones provenientes de hibridações efetuadas em 1965 Sugarcane breeding: performance of iac sugarcane clones originated from hybridation

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    Raphael Alvarez

    1987-01-01

    Full Text Available Objetivando estudar dezoito clones de cana-de-açúcar provenientes de hibridações efetuadas em Ubatuba, SP, em 1965, tendo como padrão as variedades comerciais NA56-79 e CB41-76, efetuou-se um experimento em latossolo roxo na Usina Santa Lydia, em Ribeirão Preto, SP. No ensaio, plantado em março de 1973, utilizou-se o delineamento em blocos casualizados com quatro repetições, sendo a análise estatística feita com a média das três colheitas (cana-planta, soca e ressoca. Avaliou-se a produção agrícola, teor de açúcar provável e produtividade de açúcar provável. O clone IAC65-55 apresentou produtividade de açúcar significativamente superior ao padrão CB41-76, enquanto os clones IAC65-220, IAC65-257, IAC65-255, IAC65-155 e IAC65-113 não diferiram significativamente dele. Nenhum desses clones diferiu da variedade NA56-79 em produtividade de açúcar. Em função dessas características, associadas à resistência ao "carvão", os clones IAC65-55, IAC65-257, IAC65-113, IAC65-155 e IAC65-255 poderão constituir alternativas para o cultivo na região de Ribeirão Preto.A group of 18 of the best sugarcane clones obtained from hybridation in 1965, at the Experimental Station of Ubatuba, Instituto Agronômico, were evaluated in one experiment carried out in Santa Lydia mill, Ribeirão Preto, State of São Paulo, Brazil. In 1973, it was started a field trial using as controls the commercial varieties NA56-79, and CB41-76. The experiment design was a randomized complete block with four replications. The cane yield (t/ha, sugar content (kg/t cane, and sugar yield (t/ha were expressed as the average of three harvests: cane plant (18 months, first ratoon (12 months after and second ratoon (12 months after. According to these characteristics the clones IAC65-55, IAC65-257, IAC65-113, IAC65-155 and IAC65-255 were selected, and constitute alternatives of new varieties for the sugarcane cropping in the region of Ribeirão Preto.

  17. Genitores potenciais para hibridações identificados por divergência genética em feijão carioca Bean parents for hybridization identified by genetic divergence in "carioca" bean

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    Nerinéia Dalfollo Ribeiro

    2003-06-01

    Full Text Available Noventa genótipos de feijão carioca (Phaseolus vulgaris L. foram avaliados, em dois anos agrícolas, em Santa Maria, RS, a fim de definir quais características agromorfológicas constituem-se como melhores descritores, realizar agrupamento em função de dissimilaridade genética e de definir quais combinações híbridas mais promissoras serão obtidas para o desenvolvimento de populações segregantes. Dos 20 caracteres agromorfológicos avaliados, apenas nove (ferrugem nos legumes, acamamento, nota geral, cor do tegumento, rendimento de grãos, massa de 100 sementes, altura de inserção do primeiro legume, altura de inserção do último legume e número de sementes por legume apresentaram maior contribuição para a divergência genética. Os genótipos de feijão carioca foram agrupados pelo método hierárquico de ligação completa. Populações segregantes, com variabilidade genética superior, podem ser obtidas com hibridações entre o genótipo ESAL 550 com genótipos do grupo 2 (LH-6, 17-4-32, R-78, H-4-5 e R-102 e/ou com genótipos do grupo 3 (FT 97-188, Cati-Taquari, CII-328, Carioca Precoce, FT 97-41, LH-11, FT 91-4067, Iapar 31, CI 102, Carioca MG, CII-54 e R-102.Carioca bean genotypes (Phaseolus vulgaris L. were evaluated in two growing seasons in Santa Maria, RS, Brazil. The objectives of this work were to evaluate which morpho-agronomic characteristics were the best descriptors, to group the genotypes in relation to genetic diversity and to determine which hybrid combinations are promissing to obtain higher segregation populations in carioca bean. From the 20 morpho-agronomic characteristics evaluated, only seven (pod rust, lodging, general note, colour of seed tegument, grain yield, 100 seed weight, height of first and final pod insertion and number of seeds per pod showed higher contribution to genetic diversity. The evaluated carioca bean genotypes were clustered by the complete linkage method. The following hybrid

  18. Hibridações entre Coffea arabica e C. kapakata. análise citológica de um híbrido triplóide Crossability between C. arabica and C. kapakata and cytological analysis of the hybrid

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    L. C. Mônaco

    1965-01-01

    Full Text Available Os cruzamentos realizados entre Coffea arabica e C. kapakata revelaram que há possibilidade de hibridação entre estas duas espécies e que o pegamento é bem mais elevado quando C. arabica é usada como planta feminina. Os resultados das hibridações realizadas, acrescidos aos já existentes sôbre a relação de compatibilidade de C. kapakata com outras espécies, vêm reforçar a sua inclusão dentro do gênero Coffea. As observações feitas na meiose de um híbrido triplóide entre C. arabica e C. kapakata demonstraram a formação de 1,33III 9,45II e 10,07I em média, para diacinese e primeira metáfase. As separações anafásicas são irregulares, bem como as tétrades produzidas. Os grãos de pólen contêm, em sua maioria, 13 a 17 cromossomos. Os híbridos triplóides são vigorosos e vêm sendo utilizados no melhoramento, visando introduzir nos cultivares de C. arabica a resistência à sêca presente em C. kapakata.The ability of C. kapakata to cross with C. arabica was analysed in the present paper. The ratios between seed-ovules, seedling-seeds and seedling-ovules were taken into account in order to evaluate the kinhood of the two species. It was found that the crossing rate was higher when C. arabica, the tetraploid species, was used as female. In the backcrosses it was found that the hybrid could not be crossed with C. kapakata, while C. arabica crosses in both directions with the F, hybrid. The hybrids were morphologically intermediate to both species. Observations were made about the meiosis of one of the hybrids. The chromosome associations were in average 1.33III 9.45II and 10.07I, for diakinesis and first metaphase. The distribution of the chromosomes during anaphase as well as the tetrades are abnormal. The pollen grains contain more frequently from 13 to 17 chromosomes. The hybrids between C. arabica and C. kapakata have been used as bridge for transferring drought resistance of C. kapakata to the cultivars of C

  19. Pengaruh Pemberian Mikroba Probiotik Lokal terhadap Peningkatan Produksi Jagung Hibrida

    Directory of Open Access Journals (Sweden)

    KARSIDI PERMADI

    2015-09-01

    Full Text Available Local Probiotics Microbial Effect on Improvement of Hybrid Maize Production.Nationalmaize needs continue to increase every year, either for food, feed and industrial raw materials. To meetthis requirement Indonesia have to do import corn because the production at the farm level is still lowdue to poor application of resource integrated crop management (ICM including the use of localprobiotic microbes.This study aims to determine the effect of applying maizeintegrated resourcemanagement ( ICM - maize using Local Probiotic Microbial fertilizer. The experiment was conductedin the village Cicurug, Majalengka Subdistricts, District Majalengka using a Randomized Block DesignComplete (RCBD, with four treatments and six replications. The treatment of local probiotic microbeswere: the dose of 3 l ha-1 Agri Simba, the dose of 3 l ha-1 Agri Simba + 1.500 g of urea, the dose of 6l ha-1 Agri Simba, and a dose of 6 l ha-1 Agri Simba + 3.000 g of urea. The results showed that the doseof applying local probiotic microbes (Agri Simba has significant effect on growth, yield componentsand yield of hybrid maize varieties P-21.The highest yieldof dried shelled beans was 12.20 t ha-1achievedby the dose of 6 l ha-1Agri Simba+ 3.000 g urea.The lowest yield was 8.73 t ha-1 that obtained at doseof 3 l ha-1 Agri Simba. The componentsthat support tne yield of theP-21 hybrid maize wereweightofcob, number of rows per ear and weight of 100 grains.

  20. Hybrid repowering of transformers using aramids; Repotenciacao hibrida de transformadores com utilizacao de aramida

    Energy Technology Data Exchange (ETDEWEB)

    Neri Junior, Almir Laranjeira; Correia, Fidelis Botelho [Petroleo Brasileiro S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil)

    2010-01-15

    Santiago substation of the PETROBRAS, operated with two transformers fabricated in 1971. To preserve the reliability of power supply, it was relaborated a reformation and repowering project of the equipment using aramids, a special polymeric material. This paper describes the phases of the project, and presents the benefits obtained, and also the characteristics of the electric system of the production unit. (author)

  1. Impurezas e Hibridações: Textos em Transformação

    Directory of Open Access Journals (Sweden)

    Maria Nazareth Soares Fonseca

    2002-12-01

    Full Text Available Este ensaio pretende discutir os “lugares da memória”, em que a perda da memória coletiva se configura também como indicação de possíveis formas de se restaurar o passado através de “memórias imaginadas”. A literatura pode se construir com a força da palavra falada, com as modulações das tradições ancestrais, e assumir características dos “lugares de memória”, como se pode ver no diálogo com tradições da oralidade empreendido pelos escritores René Depestre, do Haiti, e pelos angolanos Ruy Duarte de Carvalho e Paula Tavares. Os textos analisados tocam em processos de disseminação de tradições e, cada um a seu modo, constrói uma relação de reverência ao passado ao inseri-lo no mundo contemporâneo.

  2. PERFORMA IKAN PATIN HIBRIDA PASUPATI (PANGASIID DARI INDUK TERSELEKSI PADA SISTEM BUDIDAYA BERBEDA

    Directory of Open Access Journals (Sweden)

    Evi Tahapari

    2016-11-01

    Penelitian ini bertujuan untuk menguji performa ikan patin Pasupati II pada segmen pembesaran yang dilakukan di kolam air tenang (KAT berukuran 50 m2, dan di jaring (berukuran 5 m x 3 m x 1,5 m yang dipasang di tambak air payau (TAP, salinitas < 10 ppt. Ikan uji yang digunakan adalah ikan patin Pasupati II, Pasupati I, dan patin Siam F-1 dengan bobot awal di KAT 11,1-16,1 g/ekor, dan di TAP 21,3-32,5 g/ekor. Sebanyak dua KAT, dan dua jaring di TAP digunakan untuk setiap kelompok ikan. Parameter yang diamati meliputi: pertambahan bobot dan panjang harian, konversi pakan, sintasan, kualitas air pemeliharaan, dan konsentrasi hormon Insuline-like Growth Factor (IGF-I pada plasma darah. Hasil penelitian menunjukkan bahwa performa ikan patin Siam F-1 pada parameter pertambahan bobot memberikan yang terbaik (P<0,05 dibandingkan patin Pasupati I dan II yang dipelihara di KAT dan di TAP. Kemudian performa pertambahan bobot ikan patin Pasupati II lebih baik (P<0,05 daripada patin Pasupati I yang dipelihara di TAP. Hasil analisis ELISA pada beberapa ikan uji yang dipelihara di KAT menunjukkan bahwa konsentrasi hormon IGF- 1 tertinggi terdapat pada ikan patin siam F-1 (4,48 ± 0,81 ng/mL, kemudian diikuti oleh patin Pasupati II (3,96 ± 0,51 ng/mL; dan terendah pada ikan patin Pasupati I (3,93 ± 0,54 ng/mL. Jika dicermati dari data pertumbuhan dan konsentrasi hormon IGF-1 ikan uji ternyata terdapat korelasi yang positif antara pertumbuhan ikan dengan konsentrasi hormon IGF-1, semakin tinggi tingkat pertumbuhan ikan maka semakin tinggi konsentrasi hormon IGF-1 pada ikan uji. In thenursery, Pasupati II catfish hybrid of female Siamese catfish and male Jambal catfish showed the best performance compared to Pasupati I catfish and Siam catfish F-1 generation. The aim of this study was to test performance of Pasupati II on grow out segment in freshwater pond (KAT measure 50 m2 and in net cage (measure 3 m x 5 m x 1.5 m which settled in brackishwater pond (TAP, salinity < 10 ppt. The fish that used were Pasupati II, Pasupati I, and Siam catfish F-1 with body weight of 11.1-16.1 g/fish in KAT, and 21.3-32.5 g/fish in TAP. A total of two KAT, and two TAP were used for each group of fish. Parameters observed were included daily body weight and length, feed conversion ratio (FCR, survival rate, water quality, and concentration of insulin-like growth factor (IGF-1 hormone on plasma. The results showed that the performance of Siamese catfish F-1 growth parameters give the best weight gain (P<0.05 than catfish Pasupati I and II were maintained at KAT and TAP. Then the weight gain performance catfish Pasupati II was better (P<0.05 than that of catfish Pasupati I reared in TAP. Results of ELISA analysis on some of the fish that are reared in the KAT were showed that the concentration of the hormone IGF-1 was highest in F-1 Siamese catfish (4.48 ± 0.81 ng/mL, followed by Pasupati catfish II (3.96 ± 0.51 ng/mL and the lowest in the Pasupati catfish I (3.93 ± 0.54 ng/mL. When the data of growth and IGF-1 hormone concentrations in the tested fish was examined there was a positive correlation, the higher the growth rate of the fish followed the higher concentration of the IGF-1 hormone in the test fish.

  3. PENGEMBANGAN PADI VARIETAS UNGGUL HIBRIDA: PENDEKATAN METODE QUALITY FUNCTION DEVELOPMENT DAN SENSITIVITY PRICE ANALYSIS

    Directory of Open Access Journals (Sweden)

    Agrivinie Rainy Firohmatillah

    2012-06-01

    Full Text Available One of the way to increase the rice production is the development of using hybrid paddy which is can increase 15-20 percent higher than inbrid rice production. This research was aimed at identifying customer wants and preferences of hybrid paddy attributes, applying Quality Function Deployment method in developing hybrid paddy, analyzing price sensitivity hybrid paddy seed at farm gate price. This research was conducted on April to May 2011. Primary data was collected from 30 costumers (rice field farmers that knows better to hybrid paddy and ciherang paddy the competitor and four hybrid paddy breeders in Balai Besar Penelitian Padi. Quality Function Deployment and Price Sensitivity were applied to analyze the evidence. The results show that the quality of hybrid paddy is need more attention on some attributes. However, the first priority on attribute that should be improved to increase the quality of hybrid paddy is the productivity level.

  4. A hibridação interespecífica no melhoramento do cafeeiro

    Directory of Open Access Journals (Sweden)

    A. J. Teixeira Mendes

    1951-12-01

    Full Text Available The cultivation of coffee in the American coffee producing countries is not faced with any serious handicap. The future can not be far distant, however, when it will be necessary to establish new plantations back on the high-priced old exploited lands. Need will be felt for improved varieties. At the same time, it is not known if in a near future the American coffee growers will not be in trouble with diseases as terrible as Hemileia, which the present age of transport cannot restrict to non-American areas. In the program of coffee improvement, interspecific crosses are expected to take a very important place. The introducing of desired characteristics of other species into Coffea arabica by hybridization is made difficult by the fact that, while C. arabica is self-fertile, the other species are self-sterile. It is known, also, that the high cup quality C. arabica species, with 44 somatic chromosomes, gives sterile triploid hybrids (2n = 33 when crossed to the leaf disease resistant Coffea canephora and other diploid (2n = 22 species. Very seldom, however, do the triploids produce seed, as a result of back-cross to C. arabica either through open or controlled pollination. The cytological constitution of the plants obtained from these seeds is variable ; some triploids give rise to seedlings having mostly a chromosome number around 44 ; the progeny plants from other hybrids have this number mostly around 55. Numerous aneuploids have been found among these progenies. Attention has been given to the plants derived from the hybrids as to their developmental and production characteristics. At least two 44-chromosome plants have shown to be interesting in these respects and one of them, productive and self-fertile, showed up characteristics very similar to the maragogipe variety of C. arabica. More detailed comparative observations are necessary ; however, the obtaining of such a plant proves that the triploids are a permanent source of new cytological combinations that can be used in the future in the search for new vigourous and disease resistant coffee plants with high cup quality.

  5. Periode Kritis Kompetisi Gulma pada Dua Varietas Jagung (Zea mays L) Hibrida

    OpenAIRE

    Simaremare, Fitri Susi Yanti

    2011-01-01

    The competition between weed and plant can influence the decreasing of production. The uncorrect weeding can make looses. Critical period of DK 979 maize and P12 wasn’t knew yet in this area. Therefore, a research was held in Tanjung Slamat village,Sunggal, Medan (± 57 m asl) on March until July 2010 that use non factorial randomized with 12 time weeding effects (clean weeding for a week after planting (1 MST) : W1, clean weeding for 3 weeks : W2, clean weeding for 5 weeks : W3, clean weeding...

  6. PERAKITAN VARIETAS HIBRIDA JAGUNG MANIS BERDAYA HASIL TINGGI DAN TAHAN TERHADAP PENYAKIT BULAI

    OpenAIRE

    Sriani Sujiprihati; Muhamad Syukur; R Neny Iriany

    2012-01-01

    Sweet corn is a result of recessive mutation which occurs naturally in gene that controls the conversion of sugar into starch in corn seed endosperm. There are 3 main genes that affect corn sweetness, namely sugary gene (su), sugary enhancer gene (se), and shrunken gene (sh2). This study aims to obtain a general combining ability value for the sweet corn lines, which will be the parent in diallel crosses; obtain the information of general combining ability, specific combining ability, and het...

  7. PERAKITAN VARIETAS HIBRIDA JAGUNG MANIS BERDAYA HASIL TINGGI DAN TAHAN TERHADAP PENYAKIT BULAI

    Directory of Open Access Journals (Sweden)

    Sriani Sujiprihati

    2012-12-01

    Full Text Available Sweet corn is a result of recessive mutation which occurs naturally in gene that controls the conversion of sugar into starch in corn seed endosperm. There are 3 main genes that affect corn sweetness, namely sugary gene (su, sugary enhancer gene (se, and shrunken gene (sh2. This study aims to obtain a general combining ability value for the sweet corn lines, which will be the parent in diallel crosses; obtain the information of general combining ability, specific combining ability, and heterosis value for sweet corn lines; and obtain one or more promising single cross hybrids that will be potential for high yield and resistance to downy mildew disease. The study was conducted from April 2009 until November 2011 at the Experimental Field Centre for Cereal Plant Study (BALITSEREAL, Maros, South Sulawesi. The results showed that five genotypes had moderately high yield: Mr4/SC/BC4-3-1B, Mr14/SC/BC4-6-1B, Mr11/SC/BC4-3-1B, Mr14/SC/BC3-8-1B, and Mr12/SC/BC4-6-1B. The attack level of downy mildew that was classified as resistant has 0 to 7% rate. The combination of crosses that produce better productivity than all the varieties are: C × A (Mr11/SC/BC4-2-1B-1 × Mr12/SC/BC4-5-B-1, A × D (Mr12/SC/BC4-5-B-1 × Mr11/SC/BC4-2-1B-1, E × B (Mr12/SC/BC3-3-1B -1 × Mr14/SC/BC4-6-1B-1, and B × D (Mr11/SC/BC4-2-1B-1 × Mr14/SC/BC4-6-1B-1 . Five hybrids can be continued for the preliminary testing of yields. Twelve candidate varieties do not have yield different than the checked varieties, so it can proceed to a multilocation trials to explore the advantages that can be released as new varieties.

  8. Solution of optimization problems using hybrid architecture; Solucao de problemas de otimizacao utilizando arquitetura hibrida

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, Lelis Tetsuo

    2008-07-01

    Electrical power could be considered as one of the economy propulsion vector of a country, assuming extremely important and strategic role because it makes direct influence to the production capacity. The expansion of electrical energy production could not only be done in a short time because constructions in this area take many years and could require more then a decade depending on the magnitude of them. The national power generation group is constituted mainly by hydro power plants complemented by thermal power plants which use several kinds of fuel which generation cost is high, if compared to hydro power generation, and should be minimized. It is a complex planning issue to supply the future power demand which basically depends on the analysis of two compoundable scenarios: the first one refers to the forecast of future economy growing and in this case, unless un predicted issues occur such as the recent high economy growing experimented by China, the future demand does not show any surprise and is easy to predict; the second one, has inside the uncertainty because the hydro plants productions depends on the water quantity of rivers which depends on the past and current rainfall regimen. The quantity of rainfall is a stochastic data and follows the rules of probability and this drives to the study of cases and its deployments which are numerous causing difficulties to forecast the future. The planning of the electrical area has to examine the future demand and provide the necessary power generation equipment assuming a certain risk. To have it done, simulation models are used to predict the future, combining the two scenarios cited before, and viewing the results promoted by decision took in a step before. The difficult of this task is caused by the big amount of future alternatives provided by the combinatorial phenomena which require, to process the model, a computer with high processing capacity and specialized and specific methods that can resolve this king of problem. Because of the importance and magnitude of this issue, every effort which contributes to the improvement of power planning is welcome and this corroborates with this thesis which has an objective to propose technical, viable and economic solutions to solve the optimization problems with a new approach and has potential to be applied in many others kind of similar problems. (author)

  9. Efikasi Chitosan untuk Memperpanjang Flower Longevity Bunga Anggrek Dendrobium Hibrida dalam Pot (Potted Flower

    Directory of Open Access Journals (Sweden)

    I MADE SUKEWIJAYA

    2015-09-01

    Full Text Available Effication of Chitosan on Lengthening The Flower Longevity of Potted Orchid ofDendrobium Hybrid. The aim of the current research is to investigate general effects of Chitosantreatment on the flowering of Dendrobium orchid and to find out the optimum concentration of Chitosanin lengthening flower longevity of potted orchid of Dendrobium hybrid. Results of the research showedthat Chitosan application significantly affected variables of the number of flower per-plant, the length ofindividual flower, period of time to get full blooming, and the flower longevity. The best results for thoseof variables was achieved with Chitosan concentration of 0.15%.

  10. Stem Cells and Cancer; Celulas madre y cancer

    Energy Technology Data Exchange (ETDEWEB)

    Segrelles, C.; Paraminio, J. M.; Lorz, C.

    2014-04-01

    Stem cell research has thrived over the last years due to their therapeutic and regenerative potential. Scientific breakthroughs in the field are immediately translated from the scientific journals to the mass media, which is not surprising as the characterisation of the molecular mechanisms that regulate the biology of stem cells is crucial for the treatment of degenerative and cardiovascular diseases, as well as cancer. In the Molecular Oncology Unit at Ciemat we work to unravel the role of cancer stem cells in tumour development, and to find new antitumor therapies. (Author)

  11. MECANISMOS DE TRANSDUCCION ESTIMULOS MECANICOS EN CELULAS CILIADAS DE OVIDUCTO.

    OpenAIRE

    ANDRADE MONTERO, YANIRE NATY

    2004-01-01

    Los epitelios mucociliados de las vías respiratorias y del tracto genital femenino de mamíferos, contienen células ciliadas y células secretoras que forman la unidad funcional responsable del transporte mucociliar, cuya principal función es constituir un 133p.

  12. Ensayos triaxiales de rocas en celula de gran capacidad

    OpenAIRE

    Casellas Ferrer, Javier

    2013-01-01

    El trabajo realizado en esta tesina se centra en la realización de una serie de ensayos a probetas de roca para poder determinar sus características mecánicas principales. Se realizan ensayos destinados a estudiar los fenómenos de fractura en el campo de lo smateriales cuasi-frágiles, ensayo Wedge Splitting Test. Adicionalmente en esta tesina serealizarán ensayos de compresión, tanto compresión simple como compresión triaxial en una celda de confinamiento de alta capacidad utilizando una pren...

  13. INTEGRACION DE UNA CELULA FLEXIBLE DE MECANIZADO, DE TIPO DOCENTE

    OpenAIRE

    2007-01-01

    Este trabajo trata sobre la integración de una célula flexible de mecanizado de tipo docente, compuesta por una fresadora didáctica CNC, marca Denford, un brazo robótico articulado de seis ejes, modelo Scorbot -ER Vplus  y un riel deslizante; todos pertenecientes al Laboratorio de Manufactura Integrada por Computador, de la Escuela de Ingeniería Mecánica de la Universidad de Talca. Las tareas que realiza esta célula flexible son controladas por un PC director  a través de un  programa, utiliz...

  14. Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L. Varietas Hibrida BISI-2

    Directory of Open Access Journals (Sweden)

    I GUSTI NGURAH RAKA

    2013-08-01

    Full Text Available This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.

  15. EL EFECTO ANSIOLITICO Y ANTIDEPRESIVO DE LA DESIPRAMINA EVITA Y REVIERTE LOS EFECTOS DEL STRES SOBRE LA MORFOLOGIA DENDRITICA, LA EXPRESION DEL ARNM Y PEPTIDO CRH EN LA AMIGDALA Y EL LECHO DE LA ESTRIA TERMINAL

    OpenAIRE

    SANTIBAÑEZ TORO ; MARCOS ALEJANDRO

    2009-01-01

    La correcta percepción ,. experiencia de las emociones es fundamental para la sobrevivencia en un medio natural y social. Las emociones son fenómenos psicofísiológicos que. desde el punto de vista evolutivo. representan modos eficaces de adaptación a demandas ambientales cambiantes, que constituyen los desafíos adaptativos (oportunidades o amenazas). Las emociones negativas como ansiedad y miedo son ventajosas frente a situaciones amenazantes, mientras que las emociones po...

  16. EL EFECTO ANSIOLITICO Y ANTIDEPRESIVO DE LA DESIPRAMINA EVITA Y REVIERTE LOS EFECTOS DEL ESTRES SOBRE LA MORFOLOGIA DENDRITICA. LA EXPRESION DEL ARNM Y PEPTIDO CRH EN LA AMIGDALA Y EL LECHO DE LA ESTRIA TERMINAL

    OpenAIRE

    SANTIBAÑEZ TORO, MARCOS ALEJANDRO

    2009-01-01

    La correcta percepción y experiencia de las emociones es fundamental para la sobrevivencia en un medio natural y social. Las emociones son fenómenos psicotisiológicos que desde el punto de vista evolutivo, representan modos eficaces de adaptación a demandas ambientales cambiantes, que constituyen los desafíos adaptativos (oportunidades o amenazas). Las emociones negativas como ansiedad y miedo son ventajosas frente a situaciones amenazantes, mientras que las emociones positivas como ...

  17. Biomass gasification and fuel cells: system with PEM fuel cell; Gaseificacao de biomassa e celula a combustivel: sistema com celula tipo PEMFC

    Energy Technology Data Exchange (ETDEWEB)

    Sordi, Alexandre; Lobkov, Dmitri D.; Lopes, Daniel Gabriel; Rodrigues, Jean Robert Pereira [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Fac. de Engenharia Mecanica], e-mail: asordi@fem.unicamp.br, e-mail: lobkov@fem.unicamp.br, e-mail: danielg@fem.unicamp.br, e-mail: jrobert@fem.unicamp.br; Silva, Ennio Peres da [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Fisica Gleb Wataghin], e-mail: Lh2ennio@ifi.unicamp.br

    2006-07-01

    The objective of this paper is to present the operation flow diagram of an electricity generation system based on the biomass integrated gasification fuel cell of the type PEMFC (Proton Exchange Membrane Fuel Cell). The integration between the gasification and a fuel cell of this type consists of the gas methane (CH4) reforming contained in the synthesis gas, the conversion of the carbon monoxide (CO), and the cleaning of the gaseous flow through a PSA (Pressure Swing Adsorption) system. A preliminary analysis was carried out to estimate the efficiency of the system with and without methane gas reforming. The performance was also analyzed for different gasification gas compositions, for larger molar fractions of hydrogen and methane. The system electrical efficiency was 29% respective to the lower heating value of the gasification gas. The larger the molar fraction of hydrogen at the shift reactor exit, the better the PSA exergetic performance. Comparative analysis with small gas turbines exhibited the superiority of the PEMFC system. (author)

  18. Development of solid oxide fuel cells; Desenvolvimento de celulas a combustivel do tipo oxido solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Boaventura, Jaime S.; Alencar, Marcelo Goncalves F. de; Amaral, Alexandre Alves do; Benedicto, Joao Paulo Santos; Silva, Marcos A. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica. Dept. de Fisico-Quimica

    2006-07-01

    Fuel cells allow the energy production without the thermodynamic restriction of the conversion of heat into work. Among their various types, the solid oxide fuel cells (SOFC), operating at high temperatures, allow the methane conversion into electricity directly on the anode. The main element of the SOFC is the structure A/E/C: anode/electrolyte/cathode, all sintered at high temperature as resistant ceramic materials. Dense electrolyte (YSZ: zirconia stabilized for Yttria) separates the anode (Ni+Co/YSZ: cobalt promoted nickel, supported on YSZ) and cathode (LSM: strontium-doped lanthanum manganite), both with porosity obtained by graphite addition. To obtain suitable A/E/C pellets, the layer sintering with appropriate mechanical and textural characteristics is essential, requiring excellent electric junctions between them. The cell performance has been evaluated between 850 and 950 degree C, using hydrogen or methane fuel; the tension and current for different resistance values in the electrical circuit have been measured. The cobalt addition to the cell anode significantly increased its activity for the reform reaction. The beneficial effect was probably due to the easier nickel reduction in cobalt presence. This work had the objectives of developing and evaluating electro-catalysts, as well as the solid oxide fuel cells using these catalysts as anode. Five SOFC models (SOFC 1 to SOFC 5) are described; all of them were developed aiming at improving the preparation of the anode/electrolyte/cathode structure (A/E/C). (author)

  19. Efficiency of SOFC type fuel cells; Eficiencia de celulas combustiveis do tipo SOFC

    Energy Technology Data Exchange (ETDEWEB)

    Amaral, Alexandre Alves do; Matos, Francisco F.; Boaventura, Jaime S.; Benedicto, Joao Paulo S.; Alencar, Marcelo [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica. Dept. de Fisico-Quimica

    2006-07-01

    production of electric energy, mainly due to the fact that it is virtually no pollutant. Typically, the SOFC is constituted of at least seven distinct phases: fuel, anode, electrolyte substrate (separating the two electrodes), cathode, air, and electrical inter connectors (completing the electrical circuit). Thermodynamics clearly shows that electrochemical systems only can be reversible when homogeneous, what it is not case of the Sofc. Therefore, the application of equilibrium thermodynamics to these systems is incorrect. This work proposes that the Sofc can be better depicted from reactions between adsorbed species. The efficiency then is calculated as the ratio between the free energy of these reactions to the combustion heat. Thermodynamic parameter estimative is developed for the global and adsorbed species reactions. (author)

  20. TRANSPORTE DE UDP-GALACTOSA EN EL APARATO DE GOLGI DE CELULAS VEGETALES.

    OpenAIRE

    NORAMBUENA MORALES, LORENA

    2004-01-01

    En vegetales, el aparato de Golgi existe en un gran número de organelos por célula, distribuyéndose homogéneamente por todo el citoplasma. La abundancia de este organelo está en directa relación con la necesidad de depósito de componentes de pared celular 106p.

  1. PROPIEDAD ANTI-ANGIOGENICA DEL FACTOR DE COAGULACION X EN CELULAS ENDOTELIALES

    OpenAIRE

    LANGE SMITH, MARIA SOLEDAD

    2013-01-01

    La angiogénesis es la formación de vasos sanguíneos desde vasos preexistentes y juega un papel importante en el desarrollo embrionario, la formación del cuerpo lúteo y endometrio, la regeneración de tejido y la cicatrización de heridas. También participa en distintas enfermedades como arterioesclerosis, diabetes, soriasis y crecimiento tumoral. Previamente se ha reportado que algunas proteínas involucradas en los sistemas de coagulación y fibrinólisis, como factor tisular, heparin...

  2. Mediastinal large cell lymphoma with sclerosis; Linfoma de grandes celulas com esclerose do mediastino

    Energy Technology Data Exchange (ETDEWEB)

    Franco, Sergio; Pulcheri, Wolmar; Spector, Nelson; Nucci, Marcio; Oliveira, Halley P. de [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Dept. de Clinica Medica; Morais, Jose Carlos [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Dept. de Patologia; Romano, Sergio [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil). Servico de Patologia

    1995-04-01

    Five cases of primary mediastinal large-cell lymphoma with sclerosis diagnosed at the University Hospital Clementino Fraga Filho (Federal University of Rio de Janeiro) between 1986 and 1994 were identified. They were studied on clinical, morphological and immuno-histochemical grounds. Clinically, the disease was characterized by the young age of the patients, mediastinal involvement by bulky disease and compressive symptoms. None of the patients had evidence of extra-thoracic disease as presentation. On morphological grounds they had evidence of extra-thoracic disease at presentation. On morphological grounds they showed a mixture of immuno blasts and large follicular enter cell with sclerosis. Three of five cases proved to be of B-cell origin. Four of five patients were treated with chemotherapy. Cases 1 and with MACOP-B, and cases 3 and 4 with Pro-MACE-cytaBOM and consolidation radiation therapy. All the patients achieved a complete remission, and are alive, free of disease, with a follow-up of 1 to 8 years. (author). 28 refs., 8 figs., 2 tabs.

  3. Membranes for hydrogen and fuel cell technology; Membranas para celula combustivel e separacao de hidrogenio

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Suzana Pereira [GKSS-Forschungszentrum, Geesthacht (Germany)]. E-mail: nunes@gkss.de

    2005-07-01

    Membranes for fuel cell were prepared using as polymer matrix sulfonated polyether ketones. New sulfonated copolymers (poly oxazoles, poly imides and poly ketones) were synthesized. Nano composites using zirconium oxide and phosphates, as well as modified silicates were obtained aiming the application on direct methanol fuel cell (DMFC). The performances of membranes containing fillers with different aspects (spherical, layers, tubular, networks) and surface modification (hydroxy, imidazole, acid oligomers) were compared. The effect of surface modification was much more pronounced than that of the aspect. A good balance of proton conductivity and methanol permeability was obtained with silicates modified with imidazole groups. Good performance in DMFC were obtained with membranes containing zirconium phosphate. Acid oligomers also led to particularly high conductivity values above 100 deg C. Polyimide membranes with H{sub 2}/CH{sub 4} larger than 100 were obtained. (author )

  4. REGULACION DEL METABOLISMO DEL GLUCOGENO TESTICULAR Y SU POSIBLE RELACION CON APOPTOSIS DE CELULAS GERMINALES MASCULINAS

    OpenAIRE

    VILLARROEL ESPINDOLA, FRANZ DOMINIK

    2012-01-01

    El glucógeno es la principal fuente de glucosa para muchos procesos biológicos. Sin embargo, esta molécula podría tener otras funciones, incluso tener efectos negativos sobre la célula. La enzima limitante en la síntesis de glucógeno es la glucógeno sintasa (GS). Es codificada por dos genes, GYSI, expresada en músculo (isoforma muscular, MGS) y otros tejidos, y GYS2, expresada mayoritariamente en hígado (isoforma hepática, LGS). La expresión de GS y su actividad han sido amplia...

  5. Tumor cells diagnostic through fractal dimensions; Diagnostico de celulas tumorais atraves de dimensoes fractais

    Energy Technology Data Exchange (ETDEWEB)

    Timbo, Christiano dos Santos

    2004-07-01

    This method relies on the application of an algorithm for the quantitative and statistic differentiation of a sample of cells stricken by a certain kind of pathology and a sample of healthy cells. This differentiation is made by applying the principles of fractal dimension to digital images of the cells. The algorithm was developed using the the concepts of Object- Oriented Programming, resulting in a simple code, divided in 5 distinct procedures, and a user-friendly interface. To obtain the fractal dimension of the images of the cells, the program processes the image, extracting its border, and uses it to characterize the complexity of the form of the cell in a quantitative way. In order to validate the code, it was used a digitalized image found in an article by W. Bauer, developer of an analog method. The result showed a difference of 6% between the value obtained by Bauer and the value obtained the algorithm developed in this work. (author)

  6. DINAMIC MITOCONDRIAL EN LA REGULACION DEL METABOLISMO DE GLUCOSA DEPENDIENTE DE INSULINA EN CELULAS MUSCULARES ESQUELETICAS

    OpenAIRE

    DEL CAMPO SFEIR, ANDREA ESTEFANIA

    2013-01-01

    El principal objetivo del metabolismo energético es la generación de adenosina trifosfato (ATP) a través de la reducción de nutrientes tanto exógenos como endógenos. El músculo esquelético utiliza una amplia variedad de nutrientes para generar ATP dependiendo del estado en que se encuentre, variadas reacciones regulan la transformación de estos a combustible celular donde la señalización de insulina tiene un papel clave. La insulina es el principal regulador de la respuesta metabólica ...

  7. Serigraphy materialization of bi facial solar cells; Metalizacao serigrafica de celulas solares bifaciais

    Energy Technology Data Exchange (ETDEWEB)

    Moehlecke, Adriano; Zanesco, Izete; Mallmann, Ana P.; Eberhardt, Dario; Pereira, Gabriel F. [Pontificia Univ. Catolica do Rio Grande do Sul, Porto Alegre, RS (Brazil). Centro Brasileiro para Desenvolvimento da Energia Solar Fotovoltaica - CB-Solar]. E-mail: moehleck@pucrs.br

    2006-07-01

    This paper presents the low cost metallization process, based on serigraphy, for the fabrication of bifacial solar cells, optimizing the metallic patches project and the solar cell considering the serigraphic pastes, projected and manufactured a metallization mask, and implanted a screen-printer.

  8. DESARROLLO DE TECNOLOGIAS PARA EL CULTIVO DE CELULAS DE PIEL PARA TRANPLANTE AUTOLOGO

    OpenAIRE

    YOUNG A., MANUEL

    2003-01-01

    En Chile se estima que, cada año se ven afectadas 268.865 personas por quemaduras que requieren atención médica. De estos, 60.494 corresponden a quemaduras del tipo AB y B que requieren de tratamiento quirúrgico y posterior rehabilitación debido a su gravedad, dándose además frecuentemente la situación crítica de decesos. Otras lesiones severas de la piel la constituyen las ulceraciones, siendo la principal causa de amputación no traumática del pie. Para casos como quemaduras, el tratamien...

  9. Asymmetric electrooptic response in a nematic liquid crystal

    Energy Technology Data Exchange (ETDEWEB)

    Dascalu, Constanta [Politechnica University of Bucharest, Bucharest (Romania)

    2001-06-01

    An asymmetric electrooptic response in nematic liquid crystal (LC) has been obtained. The liquid crystal hybrid cell was made by using a standard configuration. One of the ITO (Indium Tin Oxide) electrodes was covered with a surfactant, which induces a homeotropic alignment. The second of the indium tin oxide electrodes was covered by a thin layer of photopolymer, which was previously mixed with an acid, which favours a process of release of protons. Such cations are responsible of electrochemical process in the LC leading to an asymmetric electrooptic response, which depend on the polarity of the applied electric field. This fact is due to an internal field, which change the effective voltage thresholds for the reorientation of the liquid crystal. During the anodic polarization, the optical switching is inhibited because the effective field decreases below the threshold value. On contrary for the opposite polarization the effective field is enough to determine a homeotropic alignment. [Spanish] Se ha obtenido una respuesta electro-optica asimetrica en cristales liquidos neumaticos. La celula hibrida de cristal liquido fue construida utilizando una configuracion estandar. Uno de los electrodos ITO fue cubierto con una pelicula delgada de material organico para inducir una alineacion homeotropa. El otro electrodo ITO fue cubierto con una pelicula delgada de fotopolimero anteriormente mezclada con un acido para favorecer la emision de protones. Estos cationes son responsables del proceso electroquimico en LC, conduciendo a una respuesta electro-optica asimetrica que depende de la polaridad del campo electrico aplicado. Este efecto es originado por un campo interno que cambia el umbral efectivo del voltaje para la reorientacion del cristal liquido. Durante la polarizacion anodica, la conmutacion optica se inhibe debido a que el campo efectivo disminuye abajo del valor del umbral. Por el contrario, para la polarizacion opuesta el campo efectivo es suficiente para

  10. Transference and natural gas distribution system analysis utilizing hybrid modelling; Analise de sistemas de transferencia e distribuicao de gas natural utilizando modelagem hibrida

    Energy Technology Data Exchange (ETDEWEB)

    Calvo, Robson A.; Martinkoski, Ricardo [Centro Federal de Educacao Tecnologica do Parana (CEFET), Curitiba, PR (Brazil); Neves Junior, Flavio [Centro Federal de Educacao Tecnologica do Parana (CEFET), Curitiba, PR (Brazil). Programa de Pos-Graduacao em Engenharia Eletrica e Informatica Industrial

    2003-07-01

    The objective of this article is to apply techniques of formal specification in modelling of natural gas transmission and distribution systems. In this case the formal models are characterized by using hybrid automata. Initially the existent components in the net are modeled and represented by independent hybrid automata. The global dynamics is obtained through the product hybrid automata. Languages representing the desirable states of the system are obtained from the hybrid automata, allowing a hybrid control procedure. An automatic tool as SHIFT must be used to modelling and simulation. (author)

  11. Cold forging stem of total hip prosthesis with hybrid mechanical properties; Forjamento a frio de hastes de protese total de quadril com propriedades mecanicas hibridas

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, E.S.N.; Contieri, R.J.; Cardoso, F.F.; Cremasco, A.; Button, S.T.; Caram, R., E-mail: ederlopes@fem.unicamp.b [Universidade Estadual de Campinas (FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica. Dept. de Engenharia de Materiais

    2010-07-01

    Type {beta} Ti alloy is one of the most versatile groups of materials with regard to mechanical properties. Aspects such as alloying elements selection, mechanical processing and heat treatment routes empower these materials in applications where hybrid mechanical behavior is necessary. The aim of this study is to produce stems of total hip prostheses with hybrid mechanical properties using Ti-Nb alloys. Ingots were produced by using arc melting. Following, samples were subjected to specific heat treatment aiming to make cold forging. Sample characterization includes X-ray diffraction, scanning electron microscopy, Vickers hardness tests and tensile test. The experiments performed allowed to examine the effects of heat treatment parameters on the microstructure and mechanical behavior. Finally, results obtained show that the application of specific heat treatments of quenching and aging makes feasible the manufacturing of orthopedic devices with hybrid mechanical properties with regions where high mechanical strength was prioritized, while in others, low elastic modulus was the main concern. (author)

  12. Hybrid Technology, the best of both worlds. The Toyota case; La Tecnologia hibrida, lo mejor de los mundos. El caso de Toyota

    Energy Technology Data Exchange (ETDEWEB)

    Dijck, K. van

    2010-07-01

    Facing Global Warming, manufacturers are increasingly obliged to offer vehicles ever more efficient and respectful towards the environment in terms of consumption and CO{sub 2} emissions. Toyota in its search towards the ultimate eco-car opts today to develop the Hybrid Technology, by enhancing the performance in electric mode in the Plug-In Hybrid Vehicle. Indeed, the new battery technology ion-litio allows for an adequate autonomy in electric mode that responds to relevant part of the possible mobility needs, whilst offering a real alternative to the conventional vehicles, without requiring the user to compromise on any of his mobility expectations he is accustomed to. The Plug-In Hybrid Vehicle combines the best of both worlds. (Author)

  13. Pre-melhoramento em capsicum: identifica??o de esp?cies, hibrida??o interespec?fica e variabilidade gen?tica em caracteres de sementes

    OpenAIRE

    Alves, Silfran Rog?rio Marialva

    2015-01-01

    A presente pesquisa teve como objetivos avaliar o pot?ncial da espectroscopia no infravermelho pr?ximo (NIR) para distinguir esp?cies do g?nero Capsicum, avaliar a viabilidade de cruzamento entre subamostras de Capsicum chinense Jacq. com Capsicum annuum L. e analisar a variabilidade gen?tica a partir de par?metros gen?ticos para os caracteres: germina??o e vigor de sementes de germoplasma de C. chinense Jacq.. Para o estudo da discrimina??o de esp?cies de Capsicum com espec...

  14. RESPON MASYARAKAT DAN DAMPAKNYA TERHADAP LINGKUNGAN DARI PROGRAM SL-PTT PADI NON HIBRIDA DI KABUPATEN HULU SUNGAI SELATAN PROVINSI KALIMANTAN SELATAN

    Directory of Open Access Journals (Sweden)

    Ria Normanita

    2016-10-01

    Full Text Available Increasing the productivity of rice through the approach of Sekolah Lapang Pengelolaan Tanaman Terpadu (SL-PTT is one of the strategies that is expected to provide a greater contribution to the national rice production. Based on the results of research, the public response to the program of SL-PTT Non Hybrid Rice in Hulu Sungai Selatan was in the high category (85.71%. Factors affecting the public response to the program were the information, the field guides, the assistance component whereas those having no effects to the public response were the education, the practice, and the application of PTT. The environmental impact of the program of SLPTT Non Hybrid Rice was positive in the economic aspect, which could create welfare for farmers implementing the program. The Social aspect giving a positve impact was  through the transmission of technology adoption that could be applied to non-rice farmers who lived around the area of SL-PTT. The negative impact was the social jealousy among the people who could all not get involved in the program of SL-PTT Non Hybrid Rice.

  15. Expresión de las moléculas del Complejo Mayor de Histocompatibilidad clase II y moléculas co-estimuladoras en carcinomas orales in vitro

    OpenAIRE

    Villarroel, Mariana; Speight, Paul M.; Barrett, A. William

    2005-01-01

    El descubrimiento de que el epitelio escamoso estratificado que cubre la mucosa oral podia expresar moleculas del Complejo Mayor de Histocompatibilidad clase II en varias condiciones patologicas de tipo inflamatorio abrio la posibilidad de que los queratinocitos orales sean celulas inmunologicamente activas, las cuales pueden funcionar con .celulas presentadoras de antigenos'ñ. Para una efectiva activacion de los linfocitos T, las celulas presentadoras de antigenos requie...

  16. Evaluación del cultivo de celulas madre mesenquimales y celulas del epitelio pigmentario de la retina en un modelo organotípico de neurorretina de cerdo

    OpenAIRE

    Rodríguez Crespo, David

    2014-01-01

    A partir de los objetivos planteados en este trabajo y tras la discusión de los resultados obtenidos se ha llegado a las siguientes conclusiones: 1- Se ha conseguido poner a punto la metodología necesaria para establecer un cultivo organotípico de neurorretina con la presencia de epitelio en forma de cultivo celular, en el que se han podido cocultivar células madre mesenquimales derivadas de tejido adiposo. 2- Se ha comprobado la seguridad de las células madre derivadas de tejido adipos...

  17. Interaccion de promastigotes de Leishmania donovani con celulas de exudado peritonial de ratones mediante la influencia de la quimotripsina

    Directory of Open Access Journals (Sweden)

    Lucila Arcay

    1986-06-01

    Full Text Available Se hace un estudio de la interacción de promastigotes de Leishmania donovani con células de exudado peritoneal de ratón (c e p mediante la influencia de la quimotripsina. La adhesión de los promastigotes a las c e p fue terminal y marginal, y en observaciones hechas a partir de los 10 minutos de enfrentamiento, esta adhesión fue nula hasta los 30 minutos en el grupo tratado, y sólo a las das horas hubo un pequeño incremento (2.4% con respecto al control. Se observa marcada disminución en todos los parámetro medidos, tales como enlace, penetración, multiplicación intracelular, división de formas flageladas, en el grupo tratado. La quimotripsina favorece la formación de formas intermedias flageladas, heciendose el parásito piriforme y esférico, apareciendo una forma aberrante de extremo anterior cilindrico que semeja a una forma coanoflagelada. Se sospecha que la enzima reduce efectivamente fragmentos proteicos o péptidos, los cuales pueden haber sido tan pequeños como para esconder a otros ligandos relacionados con la adhesión macrófago-parásito.

  18. Photostability Assessment in Amorphous-Silicon Solar Cells; Determinacion de la Fotoestabilidad en Celulas Solares de Silicio Amorfo

    Energy Technology Data Exchange (ETDEWEB)

    Gandia, J. J.; Carabe, J.; Fabero, F.; Jimenez, R.; Rivero, J. M. [Ciemat, Madrid (Spain)

    2000-07-01

    The present status of amorphous-silicon-solar-cell research and development at CIEMAT requires the possibility to characterise the devices prepared from the point of view of their stability against sunlight exposure. Therefore a set of tools providing such a capacity has been developed. Together with an introduction to photovoltaic applications of amorphous silicon and to the photodegradation problem, the present work describes the process of setting up these tools. An indoor controlled-photodegradation facility has been designed and built, and a procedure has been developed for the measurement of J-V characteristics in well established conditions. This method is suitable for a kinds of solar cells, even for those for which no model is still available. The photodegradation and characterisation of some cells has allowed to validate both the new testing facility and method. (Author) 14 refs.

  19. IGF-II Y LA GONADOTROPINA CORIONICA REGULAN LA PROLIFERACION, MIGRACION E INVASION DE CELULAS DE TROFOBLASTO HUMANO.

    Directory of Open Access Journals (Sweden)

    Umana-Perez Adriana

    2011-04-01

    Full Text Available Son conocidas las propiedades del factor de crecimiento similar a la insulina IGF-II y de la hormona Gonadotropina Coriónica (hCG en la implantación y migración trofoblástica, sin embargo los mecanismos a través de los cuales ejercen sus efectos no han sido identificados. El objetivo de este estudio fue establecer la interacción potencial entre los efectos funcionales de hCG y el IGF-II en la regulación de la proliferación, migración e invasión trofoblástica. Utilizando la línea celular HTR-8/SVneo de trofoblasto extravelloso se pudo demostrar que tanto IGF-II como hCG estimulan la proliferación y migración celular sin observarse efectos aditivos en sus acciones, lo cual es indicativo independencia de sus mecanismos. En contraste, la capacidad invasiva del trofoblasto fue regulada por IGF-II y hCG, dando como resultado efectos aditivos o un potencial sinergismo entre las dos hormonas. En conclusión, nuestros resultados muestran la existencia de interacciones entre las acciones biológicas de IGF-II y hCG en la regulación de la capacidad invasiva de células trofoblásticas y contribuyen al entendimiento de la biología del trofoblasto y de su malignización.

  20. The reformation of ethanol and application to fuel cells; A reforma do etanol e sua aplicacao em celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ennio Peres da; Camargo, Joao Carlos [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Mecanica. Dept. de Energia]. E-mails: lh2ennio@ifi.unicamp.br; joaoc@fem.unicamp.br; Carolino, Iaponira Rando [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Quimica]. e-mail: yaponira@hotmail.com

    2002-07-01

    This paper presents the perspectives for using of ethanol (EtOH) obtained from the sugar cane for electric power production, through a integrated system constituted by a hydrogen generator, by using the ethanol reforming associated to a fuel cell feed with the produced hydrogen. The paper also focuses the present re-structuration of the Brazilian electric sector identifying the possibility of implantation that system.

  1. Regeneracion osea por implante de celulas madre mesenquimales derivadas de tejido adiposo humano en un modelo animal

    OpenAIRE

    Linero Segrera, Itali Marcelly

    2012-01-01

    La reconstrucción y restauración de la función esquelética continúa siendo un reto en el campo de la cirugía maxilofacial y la ortopedia. Una alternativa o terapia coadyudante a los tratamientos convencionales es la ingeniería de tejidos que surge como una posibilidad prometedora para la regeneración del tejido óseo. Con el fin de crear un modelo para la aplicación clínica de las células madre mesenquimales derivadas de tejido adiposo humano (Ad-MSCs) desarrollamos una matriz tridimensi...

  2. Studies of water electrolysis in polymeric membrane cells; Estudos de eletrolise aquosa em celulas de membrana polimerica

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira-Silva, M.A.; Linardi, M.; Saliba-Silva, A.M. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Celulas a Combustivel e Hidrogenio

    2010-07-01

    Hydrogen represents great opportunity to be a substitute for fossil fuels in the future. Water as a renewable source of hydrogen is of great interest, since it is abundant and can decompose, producing only pure H{sub 2} and O{sub 2}. This decomposition of water can be accomplished by processes such as electrolysis, thermal decomposition and thermochemical cycles. The membrane electrolysis has been proposed as a viable process for hydrogen production using thermal and electrical energy derived from nuclear energy or any renewable source like solar energy. In this work, within the context of optimization of the electrolysis process, it is intended to develop a mathematical model that can simulate and assist in parameterization of the electrolysis performed by polymer membrane electrolytic cell. The experimental process to produce hydrogen via the cell membrane, aims to optimize the amount of gas produced using renewable energy with non-carbogenic causing no harm by producing gases deleterious to the environment. (author)

  3. Development of solid oxide fuel cells (SOFC); Desenvolvimento de celulas a combustivel do tipo oxido solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Souza, F.M.B. de; Carvalho, L.F.V. de; Alencar, M.G de; Boaventura, J.S. [Universidade Federal da Bahia (DFQ/UFBA), Salvador, BA (Brazil). Dept de Fisico-Quimica. Grupo de energia e Ciencias dos Materiais], e-mail: bventura@ufba.br

    2008-07-01

    The most promising technology for generating electric power, with reduced environmental impact, is the fuel cell. This technology is virtually non-polluting and the fuel supplies can be renewable. Therefore is necessary to study the technique of preparing the entire anode / electrolyte / cathode to optimize its operation. There are still major challenges to making the SOFC economically viable. The key is the improvement of manufacturing of its components and use of materials that can simultaneously reduce costs and reduce the temperature of operation. Among the properties of the cell, was shown the dependence of the efficiency of the device on the properties of the electrolyte, particularly its thickness. The mixture of YSZ with GDC in the composition of the anode and electrolyte aims to obtain a material with greater ionic conductivity. After sintering the cell was characterized by scanning electron microscopy (SEM). (author)

  4. The electrical behaviour of commercial solar cells under low concentration; Comportamiento electrico de celulas solares comerciales a baja concentracion

    Energy Technology Data Exchange (ETDEWEB)

    Martin, N.; Chenlo, F.

    2004-07-01

    The aim of this work is to analyse the electrical behaviour of commercial solar cells under low concentration. It is important to know the ideal concentration and temperature range that can be applied.. For that purpose we have tested the electrical characteristic of several solar cells of different technologies. The temperature during testing has been controlled (25, 40, 55 y 70 degree Celsius) and also the irradiance among 250 W/m (0,25X) and 2500 W/m2 (2,5X). The efficiency curves versus temperature show a maximum near 1000W/m and a decrease tendency caused by the serial resistance of the solar cell. The influence of temperature also function of the irradiance. (Author)

  5. Fuel cells cathode with multiple catalysis and electrocapillary convection; Catodo de celula a combustivel com catalise multipla e conveccao eletrocapilar

    Energy Technology Data Exchange (ETDEWEB)

    Bambace, Luis Antonio Waack; Nishimori, Miriam; Ramos, Fernando Manuel [Instituto Nacional de Pesquisas Espaciais (INPE), Sao Jose dos Campos, SP (Brazil)], e-mail: bambace@dem.inpe.br; Bastos Netto, Demetrio [Instituto Nacional de Pesquisas Espaciais (INPE), Cachoeira Paulista, SP (Brazil)

    2004-07-01

    This paper discusses a mathematical model for the chemical reactions and liquid phase flow processes occurring in a fuel cell cathode through non homogeneous catalysis carried by gold and Prussian Blue. The gold is applied inside the porous walls of micro-tubes, which may be obtained through several methods. The wall porosity ranging from 7 to 30% ensures gas exchange between the interior of a micro-tube and its exterior where gas flow takes place. The Prussian Blue consists of a thin porous layer located between the selective membrane and the micro-tube system, with void fraction in the 70 to 80% range. A porous electricity conducting carbide flux collector is placed between the tube system and the bipolar plates. The system return tubes possess a diameter much larger than one of the micro-tubes. The electric potential differences generated by the ionic currents in the system and its asymmetrical shape are used to generate electrocapillary flows, which are related with the surface tension changes with local potential. The hydrogen peroxide concentration and its transport to the Prussian Blue layer, and the oxygen transport inside the reactive tubular system are analyzed in this work. (author)

  6. INGENIERIA DE PROTEINAS DE UAN BETA-1,3-ENDOGLUCANASA LITICA CAPAZ DE PERMEABILIZAR CELULAS DE LEVADURA

    OpenAIRE

    OLIVERA NAPPA; ALVARO MARIA

    2005-01-01

    La endo-Beta ,3-glucanasa Bgl ll de C. cellulans (O. xanthineolytica) se presenta como una buena alternativa para ser utilizada como enzima modelo de un grupo amplio de glicosil hidrolasas, por lo que los estudios efectuados sobre ella pueden ser fácilmente extrapolables a otras enzimas, dentro de las cuales pueden contarse enzimas de claro interés industrial. Muchos procesos biotecnológicos en los que estas enzimas pueden ser de utilidad operan bajo condiciones de pH no óptimas para su acti...

  7. Electrochemical characterization of proton exchange membrane fuel cells; Caracterizacao eletroquimica de celulas a combustivel de membrana polimerica trocadora de protons

    Energy Technology Data Exchange (ETDEWEB)

    Furtado, Jose Geraldo de Melo; Serra, Eduardo Torres [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)]. E-mail: furtado@cepel.br; Codeceira Neto, Alcides [Companhia HidroEletrica do Sao Francisco (CHESF), Recife, PE (Brazil)

    2008-07-01

    This paper describes the electrochemical behavior of a proton exchange membrane fuel cell in function of temperature and time of operation. Different polarization phenomena are considered in the 30 to 70 deg C temperature range, as well as the degradation of electrochemical behavior of the fuel cell analyzed up to 1260 hours of operation. The results show that there is a tendency for the experimental values approaching the theoretical as it increases the temperature of the membrane electrolyte. The electrochemical behavior of the PEMFC studied proved to be less stable at 70 deg C. On the other hand, at 30 deg C the fuel cell performance proved to be considerably lower than at other temperatures. Also, it was found that in certain current ranges occurs greater overlay in potential-current curves, and in some cases reversing between these curves depending on the electric current required for the data obtained at 60 and 70 deg C, indicating, perhaps, that at 70 deg C the characteristics of the electrolyte are slightly inferior to those at 70 deg C, corresponding to an electrolyte degradation. Additionally, for the system studied, we found that the rate of variation of the potential difference in function of the temperature is quite high at the beginning of the operation process and tends to stabilize in a level of around 2,3-2,5 {mu}V per minute for times greater than 330 hours of operation. (author)

  8. Establecimiento de un cultivo de celulas en suspensión de Eucalyptus cinérea

    Directory of Open Access Journals (Sweden)

    Orozco Sáncnez Fernando

    2002-06-01

    Full Text Available Se desarrolla un protocolo para la obtención y establecimiento de suspensiones celulares de E. cinerea. La concentración de las hormonas 2,4 D Y BAP tienen un efecto significativo en la formación de callos friables de E. cinerea, obteniendo una respuesta periódica en la formación de callos friables con respecto a la concentración de las hormonas. Puede obtenerse hasta un 90% de formación de callos friables con varias combinaciones hormonales; primero, con concentraciones alrededor de 3,0 mg/L de 2,4 D Y 1,0 mg/L de BAp, y segundo, alrededor de 6,0 mg/L de 2,4 D Y1,0 mg/L de BAP. A partir de los callos anteriores, se obtienen suspensiones celulares con un activo crecimiento celular, con tiempos de duplicación entre cuatro y siete días, y alcanzando densidades celulares de 15 g células secas/L. Las suspensiones de E. cinerea ofrecen una herramienta importante para la propagación de esta especie vía embriogénesis somática, estudio de biorreactores, producción de metabolitos secundarios y procesos de biotransformación.

  9. Experimental study of proton exchange membrane fuel cell; Estudo experimental de uma celula a combustivel de membrana polimerica

    Energy Technology Data Exchange (ETDEWEB)

    Acevedo, Luis Evelio Garcia; Oliveira, Amir Antonio Martins [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica], e-mail: evelio@labcet.ufsc.br, e-mail: amirol@emc.ufsc.br

    2006-07-01

    Here we present a thermodynamic and chemical kinetic analysis of the methane steam reforming for production of 5 kw of electrical power in a PEM fuel cell. The equilibrium analysis is based on the method of element potentials to find the state of minimum Gibbs free energy for the system and provides the equilibrium concentration of the reforming products. The objective of this analysis is to obtain the range of reforming temperature, pressure and steam-methane molar ratio that results in maximum hydrogen production subjected to low carbon monoxide production and negligible coke formation. The thermal analysis provides the heat transfer rates associated with the individual processes of steam production, gas-phase superheating and reforming necessary to produce 5 kw of electrical power in a PEM fuel cell and allows for the calculation of thermal efficiencies. Then, the chemical reaction pathways for hydrogen production in steam reforming are discussed and the available chemical, adsorption and equilibrium constants are analyzed in terms of thermodynamic consistency. This analysis provides the framework for the reactor sizing and for establishing the adequate operation conditions. (author)

  10. Experimental analysis of a PEM fuel cell 15 W; Analise experimental de uma celula a combustivel PEM 15W

    Energy Technology Data Exchange (ETDEWEB)

    Miyake, Raphael Guardini; Bazzo, Edson [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica. Lab. de Combustao e Engenharia de Sistemas Termicos], e-mail: miyake@labcet.ufsc.br, e-mail: ebazzo@emc.ufsc.br

    2006-07-01

    Fuel cells have been considered a promising alternative for electric energy generation. In order to contribute with the development of this technology, a PEM fuel cell was installed and new experiments were carried out at LabCET (Laboratory of Combustion and Thermal System Engineering). Previous results have shown polarization curves identifying the need of rigorous controlling of humidification temperature of the fuel cell. In this paper, new results were carried out considering the use of a fan connected to the fuel cell and possible degradation in the electrolyte, after a relative long time operation. New polarization curves were plotted for comparison with previous results. (author)

  11. INTEGRACION DE UNA CELULA FLEXIBLE DE MECANIZADO, DE TIPO DOCENTE INTEGRATION OF A EDUCATIONAL FLEXIBLE MANUFACTURING CELL

    Directory of Open Access Journals (Sweden)

    Abraham Farias F

    2007-04-01

    Full Text Available Este trabajo trata sobre la integración de una célula flexible de mecanizado de tipo docente, compuesta por una fresadora didáctica CNC, marca Denford, un brazo robótico articulado de seis ejes, modelo Scorbot -ER Vplus  y un riel deslizante; todos pertenecientes al Laboratorio de Manufactura Integrada por Computador, de la Escuela de Ingeniería Mecánica de la Universidad de Talca. Las tareas que realiza esta célula flexible son controladas por un PC director  a través de un  programa, utilizando el software y el propio control del robot y su objetivo es realizar el ciclo de carga y descarga de un  trozo de material de 65×65×40 mm. (madera y su posterior mecanización. El problema de comunicación entre los equipos involucrados y la fresadora CNC fue resuelto en cuanto su diseño "stand alone" no contemplaba la integración con otros equipos. Por otro lado, se agregaron algunos sistemas electroneumáticos para que la operación fuera automática, como los de sujeción de piezas y la apertura y cierre de puertas. Con esta célula flexible de mecanizado de tipo docente, la facultad de Ingeniería cuenta con una plataforma básica, a la que se puede adicionar otras funciones, como por ejemplo, dotarla de un sistema automático de alimentación de piezas, de un sistema de visión digital para dar más autonomía al robot, incorporar un torno CNC y  generar un programa para administrar las órdenes de trabajo.This paper describes the integration of an educational flexible manufacturing cell, consisting of a Denford CNC mill with six axes, and a Scorbot - ER Vplus robotic arm that slides along a rail. This equipment is located at the Computer Integrated Manufacture Laboratory, at the School of Mechanical Engineering at Universidad de Talca. The flexible cell is controlled by software in a PC that interacts with the software in the robotic arm. The specific tasks described in this paper are loading and unloading a wooden piece (640×65×40 and to mechanizing the piece. One difficulty solved in this work was the potential communication problem between the control equipment CNC mill, since the original, stand-alone design did not admit integration with other controllers. In addition, electro-pneumatic devices were added to permit automatic operation of the system in situations such as gripping the piece and opening the mill door. This basic platform gives the possibility of adding new functions and equipment, such as automatic loading of work pieces, digital reading systems, a CNC lathe and a work order information system.

  12. Intraabdominal desmoplastic small round cell tumor - case report; Tumor de pequenas celulas redondas desmoplasico intra-abdominal - relato de caso

    Energy Technology Data Exchange (ETDEWEB)

    Westphalen, Antonio Carlos Anderson; Ferreira, Jose Hamilton Pinheiro [Hospital Moinhos de Vento, Porto Alegre, RS (Brazil). Moinhos Centro de Imagem]. E-mail: cpostal@zaz.com.br; Daudt, Alexander Welaussen [Centro de Oncologia Radioterapica do Rio Grande do Sul, Porto Alegre, RS (Brazil); Gaiger, Ana Maria [Pontificia Univ. Catolica do Rio Grande do Sul, Porto Alegre, RS (Brazil). Faculdade de Medicina. Dept. de Patologia

    2001-10-01

    The authors report a case of intraabdominal desmoplastic small round cell tumor in a 21-year-old male patient who presented with abdominal pain, right lower limb thrombosis and obstructive loss of renal function. Initial investigation showed a large lobulated intraabdominal soft tissue mass with internal cystic cavities, located posteriorly to the bladder and causing obstruction of ureteral flow, compression of the right iliac vein and partial occlusion of the rectum. Involvement of intraperitoneal and retroperitoneal nodes was also seen. Ultrasound, computed tomography and magnetic resonance imaging findings, as well as surgical and histopathology macroscopical, microscopical and immunohistochemistry findings are described. (author)

  13. Systems of three generation using fuel cells; Sistemas de trigeracao com o uso de celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    San Martin Diaz, Jose Ignacio; Aperribay Maiztegui, Victor; San Martin Diaz, Jose Javier [Escuela Universitaria de Ingenieria Tecnica Industrial de Eibar, Guipuzcoa, Pais Vasco (Spain); Zamora Belver, Inmaculada; Eguia Lopez, Pablo [Escuela Tecnica Superior de Ingenieria de Bilbao (Spain)

    2010-10-15

    The three generation includes production processes and simultaneous use oe electric power, heat and cold from a fuel source providing global high energy efficiency, reduction of emission of gases in the atmosphere, losses of investments, and more reliability in the supply. This paper approaches the use of fuel cells for three generation, and presents different subsystems of generation with the main characteristics and applications. (author)

  14. DESREGULACION DE LA ACTIVIDAD DEL FACTOR DE TRANSCRIPCION NF-KB MEDIADA POR CALCIO EN CELULAS MUSCULARES DISTROFICAS MDX

    OpenAIRE

    ALTAMIRANO FULLA, FRANCISCO JAVIER; ALTAMIRANO FULLA; FRANCISCO JAVIER

    2012-01-01

    La Distrofia Muscular de Duchenne (DMD) es una enfermedad genética recesiva,ligada al cromosoma X, que afecta a 1 de cada 3500 hombres nacidos. La causa de esta enfermedad es la mutación del gen de la distrofina en humanos y en ratones mdx (modelo animal de DMD). La DMD es una enfermedad progresiva que se caracteriza por daños en la membrana de las fibras musculares, infiltración de células inmunes en el músculo,inflamación crónica, daño de sarcómeros, muerte celular y degeneración severa del...

  15. Technical analysis of fuel cells applied to cogeneration systems; Analise tecnica de celulas combustivel aplicadas em sistemas de cogeracao

    Energy Technology Data Exchange (ETDEWEB)

    Pulgar, Rafael Girardi; Bazzo, Edson [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica]. E-mails: rafael@cet.ufsc.br; ebazzo@emc.ufsc.br; Martinac, Ivo [KTH - Royal Institute of Tech., Stockholm (Sweden). Dept. of Energy Technology]. E-mail: im@egi.kth.se

    2002-07-01

    In the last years the fuel cell technology has been pointed out as a promising alternative for electricity generation. Compared to conventional energy conversion processes it presents great advantages, such as high efficiency, low gases emissions and the possibility to be applied in cogeneration systems, among others. The fuel cell converts the chemical energy of the fuel directly into electricity via an electrochemical process. The waste heat generated in the process can be recovered in cogeneration applications. In this article, preliminary results related to a Phosphoric Acid Fuel cell (PAFC) applied to hotels for power generation and hot water production are presented. The PAFC shows an electrical efficiency of about 40 per cent for power generation. In the cogeneration system proposed in this work, the global thermoelectric efficiency increased up to 73 per cent, when both electrical and thermal energy are considered. (author)

  16. Aspectos cineticos e operacionais da fermentação alcoolica continua em fermentador cascata com celulas livres e imobilizadas

    OpenAIRE

    Aldo Alessandro Milanese Rubilar

    1987-01-01

    Resumo: Foram realizadas fermentações contínuos da levedura Saccharomyces cerevisiae IZ 888 ruim reator multiestágio com células imobilizadas, operando a 33°C e pH entre 2,5 - 3,0. Foi utilizada a técnica de imobilização por adsorção, usando como suporte cubos de 5x5x5 mm de esponja de polietíleno de alta densidade, em leito empacotado. A fim de conseguir características tipo CSTR, cada reator foi dotado de uma bomba centrífuga para recirculação interna . O sistema consta de 6 reatores em ser...

  17. Interaccion de promastigotes de Leishmania donovani con celulas de exudado peritonial de ratones mediante la influencia de la quimotripsina

    OpenAIRE

    Arcay,Lucila; Bruzual,Elizabeth

    1986-01-01

    Se hace un estudio de la interacción de promastigotes de Leishmania donovani con células de exudado peritoneal de ratón (c e p) mediante la influencia de la quimotripsina. La adhesión de los promastigotes a las c e p fue terminal y marginal, y en observaciones hechas a partir de los 10 minutos de enfrentamiento, esta adhesión fue nula hasta los 30 minutos en el grupo tratado, y sólo a las das horas hubo un pequeño incremento (2.4%) con respecto al control. Se observa marcada disminución en to...

  18. Evaluation of sulphonated polycarbonate membranes for fuel cells; Avaliacao de membranas de policarbonato sulfonado para celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Machado, Isabela M.M.; Gomes, Ana C.; Pessan, Luiz A. [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Engenharia de Materiais], e-mail: isabelamuglia@gmail.com; Paranhos, Caio [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica

    2011-07-01

    Fuel cells based on ion conducting polymer membranes offer an alternative for the conventional energetic matrices. Among many advantages of this system, we can mention the reduction on the emission of pollutants, high efficiency and simplicity. This work presents the modification of polycarbonate by sulfonation reaction using acetyl sulfate, in order to increase the conductor ionic character of the membranes used as electrolytes for hydrogen fuel cells. The sulfonated polycarbonate membranes were obtained by casting and then characterized by Fourier transform infrared spectroscopy, water vapor transmission, differential scanning calorimetry, thermogravimetric analysis and viscosimetry. (author)

  19. Perovskites synthesis for solid oxide fuel cells; Sintese de perovsquitas para celulas a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Sibelle F.C.X.; Melo, Dulce M.A.; Pimentel, Patricia M.; Melo, Marcus A. Freitas; Martinelli, Daniele M.H. [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. de Quimica]. E-mail: sibelle.cunha@gmail

    2008-07-01

    This work aims to study on the obtaining powders of lanthanum manganite oxides with partial substitution of La with strontium at 20% for the application as a cathode for solid oxide fuel cell, through a route of synthesis that are similar to the Pechini method, in which gelatin replaces the ethylene glycol as polymerization agent. The method highlights itself due to its simplicity, low cost and capability to obtain crystalline powders with the high purity and good stoichiometric control. The perovskite obtained were characterized by thermogravimetric analysis, X ray diffraction, electronic scanning microscopy and the superficial area by BET method. The deposition of the perovskite on electrolyte/anode system was done through the spin coating technique. The methodology used for the perovskite synthesis was very efficient, considering a monophasic material was obtained and with characteristics that were proper to the application as electrode to solid oxide fuel cells. (author)

  20. Use of fuel cells in residences and commercial establishments; Uso de celulas combustiveis em residencias e estabelecimentos comerciais

    Energy Technology Data Exchange (ETDEWEB)

    Serra, Eduardo T. [Centro de Pesquisas de Energia Eletrica, Rio de Janeiro, RJ (Brazil)

    2003-05-01

    This paper analyses the present technological stage and the economical feasibility of the application of low power fuel cells for meeting of the residence and small commercial units electric power consumption needs.

  1. REGULACION DE LA DINAMICA Y METABOLISMO MITOCONDRIAL POR INCRETINA GLP-1 EN CELULAS DE LA LINEA A7R5

    OpenAIRE

    TORRES RIVERA, GLORIA ANDREA

    2014-01-01

    El péptido similar a glucagón-1 (GLP-1) es una incretina secretada por las células intestinales. Su acción clásica es regular la respuesta secretoria de insulina por el páncreas. Sin embargo, recientemente se ha descrito que GLP-1 posee acciones extrapancreáticas muy similares a la insulina, con mecanismos de señalización tejido-específicos. Por sus muchas acciones fisiológicas, y la mayoría relacionadas con los efectos insulinotrópicos de esta incretina, derivados peptidomiméticos de G...

  2. INTEGRACION DE UNA CELULA FLEXIBLE DE MECANIZADO, DE TIPO DOCENTE INTEGRATION OF A EDUCATIONAL FLEXIBLE MANUFACTURING CELL

    OpenAIRE

    2007-01-01

    Este trabajo trata sobre la integración de una célula flexible de mecanizado de tipo docente, compuesta por una fresadora didáctica CNC, marca Denford, un brazo robótico articulado de seis ejes, modelo Scorbot -ER Vplus  y un riel deslizante; todos pertenecientes al Laboratorio de Manufactura Integrada por Computador, de la Escuela de Ingeniería Mecánica de la Universidad de Talca. Las tareas que realiza esta célula flexible son controladas por un PC director  a través de un  programa, utiliz...

  3. Performance of direct ethanol and methanol fuel cells as function of alcohol concentration applied to the anode of those cells; Desempenho de celulas a combustivel com alimentacao direta de etanol (CCADE) e celulas a combustivel com alimentacao direta de metanol (CCADM) em funcao da concentracao do alcool aplicado ao anodo destas celulas

    Energy Technology Data Exchange (ETDEWEB)

    Belchor, P.M.; Forte, M.M.C. [Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Correa, J.P.; Kuhn, C.K.; Carpenter, D. [FURB -Fundacao Universidade Regional de Blumenau, SC (Brazil)

    2010-07-01

    This paper aimed to compare the performance of a CCADE and a CCADM as function of diminishing of concentration of alcohol applied top the anode of each cell. As result, reaching a diminishing the concentration of each alcohol through the mix of each one with the deionized water, one obtained a improved humidification and sensible reduction of the permeability of cationic membrane, generating a gain of performance of the functioning both cells. (author)

  4. Fuel cell: new electrocatalysts for SOFC (Solid Oxide Fuel Cells) anodes and regulation between cell performance and catalytic activity; Celula a combustivel: novos eletrocatalisadores para anodos de SOFC (Celulas a Combustivel de Oxido Solido) e correlacao entre desempenho da celula e atividade catalitica

    Energy Technology Data Exchange (ETDEWEB)

    Boaventura, Jaime S.; Aguiar, Aurinete B.; Brandao, Soraia T. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil); Frank, Maria Helena Troise; Campos, Michel F. [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2004-07-01

    Electro-catalysts were prepared using new routes. Chemical Ultrasound Deposition (CUD) method: aqueous solution of nickel nitrate and citric acid was ultrasound vaporized and deposited on heated Ytria-stabilized Zirconia (YSZ). Resin impregnation (IPR) method: nickel oxide and YSZ were mixed, added to phenolic resins, precipitated in acidic water and milled. Wet impregnation method (IMP) was used for comparison: YSZ and an aqueous solution of nickel nitrate and citric acid were mixed, followed by evaporation, drying and calcination. The catalysts were evaluated for methane steam reforming in a quartz reactor. The reactions were conducted for one hour with no significant catalytic activity loss. In reactions with 100 mg of catalyst and a mixture consisting of methane and steam (3:1), IPR catalyst showed activity higher and better stability than those by IMP. On other tests, the reform was conducted with 100 mg of catalyst and methane to steam of 10. The IPR catalyst activity was so high that the reaction approached equilibrium conditions. Anode/electrolyte/cathode units (A/E/C) were prepared with the above catalysts as follows: the anode was a catalyst porous layer; the electrolyte an YSZ dense layer; and the cathode an LSM porous layer; graphite powder formed the material porosity. The two first layers, in powder form, were put in a stainless steel cast, pressed to 4000 bars and sinterized. The cathode layer was subsequently added using tape-casting techniques followed by sintering. A/E/C units showed 40% linear contraction and porosity higher than 20%. For fuel cell tests, A/E/C was mounted in alumina plates with platinum current collectors. Unitary SOF cells were loaded with hydrogen diluted in nitrogen showing opened circuit voltage from circa 700 mV, for the CUD anode, to 350 mV, for the IPR anode. The unitary SOFC was loaded with methane for 15 minutes or longer, with no noticeable voltage loss. At 1300 K the SOFC made with IPR or IMP catalysts showed opened current voltages close to 500 mV, while CUD SOFC measured less approximately 300 mV. In conclusion, the SOFC opened current voltage might be related to the reforming activity of the electro-catalyst used as the anode. (author)

  5. Solving optimization problems using hybrid architecture: a strategy for planning the electric power generation; Solucao de problemas de otimizacao utilizando arquitetura hibrida: uma estrategia para o planejamento da geracao de energia eletrica

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, L.T. [Lechare Informatica Ltda., Sao Paulo, SP (Brazil)

    2009-07-01

    Electrical power could be considered as one of the economy propulsion vector of a country, assuming extremely important and strategic role because it makes direct influence to the production capacity. The expansion of electrical energy production could not only be done in a short time because constructions in this area take many years and could require more then a decade depending on the magnitude of them. The national power generation group is constituted mainly by hydro power plants complemented by thermal power plants which use several kinds of fuel which generation cost is high, if compared to hydro power generation, and should be minimized. It is a complex planning issue to supply the future power demand which basically depends on the analysis of two compoundable scenarios: the first one refers to the forecast of future economy growing and in this case, unless unpredictable issues occur such as the recent high economy growing experimented by China, the future demand does not show any surprise and is easy to predict; the second one, has inside the uncertainty because the hydro plants productions depends on the water quantity of rivers which depends on the past and current rainfall regimen. The quantity of rainfall is a stochastic data and follows the rules of probability and this drives to the study of cases and its deployments which are numerous causing difficulties to forecast the future. The planning of the electrical area has to examine the future demand and provide the necessary power generation equipment assuming a certain risk. To have it done, simulation models are used to predict the future, combining the two scenarios cited before, and viewing the results promoted by decision took in a step before. The difficult of this task is caused by the big amount of future alternatives provided by the combinatorial phenomena which require, to process the model, a computer with high processing capacity and specialized and specific methods that can resolve this king of problem. Because of the importance and magnitude of this issue, every effort which contributes to the improvement of power planning is welcome and this corroborates with this thesis which has an objective to propose technical, viable and economic solutions to solve the optimization problems with a new approach and has potential to be applied in many others kind of similar problems.

  6. Somatic hybridization between Citrus sinensis (L. Osbeck and C. grandis (L. Osbeck Hibridação somática entre Citrus sinensis (L. Osbeck e C. grandis (L. Osbeck

    Directory of Open Access Journals (Sweden)

    Marcia Cristina Calixto

    2004-07-01

    Full Text Available This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.O objetivo deste trabalho foi produzir híbridos somáticos entre laranjas doces e toranjas. Após fusão de protoplastos embriogênicos de laranja doce com protoplastos derivados de mesófilo foliar de toranjas, plantas foram regeneradas por embriogênese somática e aclimatizadas em casa de vegetação. Os híbridos laranja 'Hamlin' + toranja 'Indian Red' e laranja 'Hamlin' + toranja 'Singapura' foram confirmados pela morfologia foliar, contagem do número de cromossomos e análise molecular. Esses híbridos apresentam potencial para serem testados como porta-enxertos tolerantes a declínio, tristeza e doenças causadas por Phytophthora, bem como em programas de melhoramento de porta-enxertos.

  7. Deteccion del virus del papiloma humano de alto riesgo por captura hibrida II segun hallazgos citologicos en mujeres tratadas por lesiones escamosas intraepiteliales de cuello uterino, periodo 2006/2010

    Directory of Open Access Journals (Sweden)

    Pamela Mongelos

    2013-03-01

    Full Text Available OBJETIVO: Determinar la frecuencia del virus de papiloma humano de alto riesgo oncogénico (HR-HPV por captura híbrida II (r (CH II(r según hallazgos citológicos en mujeres tratadas por lesiones escamosas intraepiteliales (SIL de cuello uterino. MATERIAL Y MÉTODO: Estudio descriptivo de corte transverso de una serie de casos, en donde se incluyeron 122 mujeres tratadas, 79 (65% por SIL de bajo grado (LSIL y 43 (35% por SIL de alto grado (HSIL que concurrieron al Laboratorio de HPV del Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, para realizarse un control post-tratamiento, periodo 2006/2010. RESULTADOS: Se observó un total del 28% (34/122 de mujeres tratadas por SIL positivas para HR-HPV, detectándose infección viral en un 20% de las mujeres con ausencia de SIL (NSIL (22/108, 83% de las mujeres con LSIL (10/12 y 100% de las mujeres con HSIL (2/2. De las 34 mujeres positivas para HR-HPV, 10 mujeres (29% presentaron valores altos (100 pg/mL o más de carga viral relativa, detectándose un aumento de casos positivos con la severidad de la lesión (28% NSIL, 30% LSIL, 50% HSIL. CONCLUSION: La detección de HR-HPV por CH II(r, así como los valores de carga viral relativa altos, en especial en mujeres con NSIL podrían ayudar a identificar mujeres tratadas con riesgo a desarrollar recidivas, contribuyendo así a fortalecer el programa de prevención de cáncer de cuello uterino.

  8. Exchange of diesel generator's retrofitting by PV-diesel hybrid plants in the south of Morocco; Sustitucion de grupos electrogenos por pequenas centrales hibridas FV-diesel en el sur de Marruecos

    Energy Technology Data Exchange (ETDEWEB)

    Munoz Cano, J.; Narvarte Fernandez, L.; Lorenzo Pigueiras, E.

    2004-07-01

    This paper describes a pilot experience of diesel generator's retrofitting by PV-diesel hybrid plants in two rural villages in the south of Morocco. After two operating years, we are ready to present the obtained results, especially, those that can be useful for future electrification projects based on this technology, such as the introduction of the energy service, the energy consumption of users and the managerial aspects that contribute to the long-term sustainability of these systems. (Author)

  9. Shyntesis and cytotoxicity evaluation in vitro of new compounds with hybrid structures of 8-flavoneacetic acid and quinolones; Sintesis y evaluacion citotoxica in vitro de nuevos compuestos con estructuras hibridas del acido 8-flavonacetico quinolonas

    Energy Technology Data Exchange (ETDEWEB)

    Biaa, M.F.; Castellano, J.M.; Emling, F.; Schlick, E. [Knoll, S.a., Madrid (Spain)

    1994-12-31

    Using the structural similarity between 8-flavoneacetic acid the antitumor quinolones, we have prepared some hybrid compounds on both systems and studied their cytotoxicity. None of the sinthesized compounds have shown sufficient interest for further development. 33 refs.

  10. Hybrid regression trees applied to the monitoring of dynamic safety of isolated networks with large eolic production contribution; Utilizacao de arvores de regressao hibridas na monitorizacao da seguranca dinamica de redes isoladas com grande producao eolica

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, J.A Pecas; Vasconcelos, Maria Helena O.P. de [Instituto de Engenharia de Sistemas e Computadores (INESC), Porto (Portugal). E-mail: jpl@riff.fe.up.pt; hvasconcelos@inescn.pt

    1999-07-01

    This paper describes in a synthetic manner the technology adopted to define structures used in the fast evaluation of dynamic safety of isolated network with high level of eolic production contribution. This methodology uses hybrid regression trees, which allows the quantification the endurance connected to the dynamic behavior of these networks by emulating the frequency minimum deviation that will be experienced by the system when submitted toa pre-defined perturbation. Also, new procedures for data automatic generation are presented, which will be used for construction and measurements of the evaluation structures performance. The paper describes the Terceira island - Acores archipelago network study case.

  11. Técnica quirúrgica hibrida de fijación externa descartable (fed) y prótesis articulada en la en la corrección de subluxacion tarso crural.

    OpenAIRE

    2006-01-01

    La subluxación tarsocrural en caninos esfrecuente en la practica clínica, seproduce por torceduras o hiperextensiónde la articulación, acompañándose con laruptura o desgarro de uno o ambosligamentos laterales, de la capsulaarticular y en ocasiones con fracturas delastrágalo o uno o ambos maléolos de latibia. La reducción puede ser abierta ocerrada. Las técnicas tradicionales deelección para resolver esta patologíadificultan la recuperación debido altiempo de inmovilización de laarticulación a...

  12. Deteccion del virus del papiloma humano de alto riesgo por captura hibrida II segun hallazgos citologicos en mujeres tratadas por lesiones escamosas intraepiteliales de cuello uterino, periodo 2006/2010

    OpenAIRE

    Pamela Mongelos; Malvina Paez; Isabel Rodriguez-Riveros; Graciela Gimenez; Amalia Castro; Laura Mendoza

    2013-01-01

    OBJETIVO: Determinar la frecuencia del virus de papiloma humano de alto riesgo oncogénico (HR-HPV) por captura híbrida II (r) (CH II(r)) según hallazgos citológicos en mujeres tratadas por lesiones escamosas intraepiteliales (SIL) de cuello uterino. MATERIAL Y MÉTODO: Estudio descriptivo de corte transverso de una serie de casos, en donde se incluyeron 122 mujeres tratadas, 79 (65%) por SIL de bajo grado (LSIL) y 43 (35%) por SIL de alto grado (HSIL) que concurrier...

  13. Production of Group II and III base oils by hybrid route using brazilian crude; Producao de oleos basicos lubrificantes dos grupos II e III pela rota hibrida ou mista a partir de petroleo brasileiro

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Wlamir Soares; Fontes, Anita Eleonora Ferreira [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES)

    2004-07-01

    This paper describes a series of pilot plant tests made at PETROBRAS Research Centre, considering hydrotreatment and solvent dewaxing steps, to produce group II and group III lube base oils from Baiano Light crude feeds (Brazilian crude). RLAM Refinery has been using Baiano light crude to produce group I base oils by conventional route and in the pilot plant studies, two types of process scheme were tested. In the first one, an industrial run was performed at RLAM Refinery, including distillation, dewaxing and extraction and the light raffinate was used as a feed for a hydrotreatment pilot plant, followed by a distillation to remove the front ends. In the second scheme, another industrial run was performed, including distillation and dewaxing steps and the medium dewaxed oil was used as a charge for a hydrotreatment followed by distillation and dewaxing pilot plant tests. Products of excellent quality were obtained. Due to their high viscosity indexes (from 96 to 126), low contaminants levels (sulfur < 5 ppm and nitrogen < 5 ppm) and low aromatic content (CA < 2 %), the lube base oils produced are therefore classified as group II and group III. The main advantages of this route are related to the base oils quality improvements with low investment and more flexibility in terms of crude source. (author)

  14. Músicas, cuerpos e identidades hibridas en el caribe: ¿cuál música, cuál cuerpo, cuál identidad, cuál caribe?

    Directory of Open Access Journals (Sweden)

    Lorena Aja

    2014-02-01

    Full Text Available En este artículo se presenta un panorama del Caribe en su identidad a partir de los artefactos culturales que engendran particularidades en su significación: la música y la danza. El cuerpo se retoma corno una amalgama de significados, de expresiones identitarias que devienen en la polifonía del ser caribe. En artículo se toma una postura conceptual sobre la identidad, la cultura y el caribe a partir de la experiencia personal del autor.

  15. Estudos sobre a regeneração do figado - variação do volume nuclear das celulas hepáticas em repouso divisional

    Directory of Open Access Journals (Sweden)

    Fernando Ubatuba

    1948-12-01

    Full Text Available Surgical removal of large amounts of hepatic tissue in male albino rats results in a rapid and conspicuous raise in cellular nuclear volumes. Measurements were made exclusively in resting nuclei. This volume variation is transitory. Nuclear volumes return to the normal value withins 6 days of restoration. The higher value are abserved 48 hours after the hepatic removal, indicating probably that this effect is due to hydration of the nucei, as occurs in the cytoplasm. This hydration could be correlated to the mitotic activity of the renmant tissue since a peak of mitoses parallels the changes in the nuclear volumes.

  16. Linfoma de celulas B de la zona marginal extraganglionar del tejido linfoide asociado a mucosa (linfoma MALT de pulmón

    Directory of Open Access Journals (Sweden)

    Carlos Vergara-Uzcategui

    2014-09-01

    Full Text Available Los Linfomas Pulmonares Primarios son extremadamente raros (0,4% de los linfomas extraganglionares, y generalmente son de tejido linfoide asociado a mucosas (tipo MALT, con ocasionales linfomas de células grandes difusos. Los síntomas son inespecíficos, y casi la mitad de los pacientes son asintomáticos. Se presenta el caso de un paciente masculino de 56 años de edad, quien presentó durante 7 meses accesos diarios de tos seca, de predominio nocturno, asociados en el último mes a fiebre de 39ºC, disnea a medianos esfuerzos y expectoración verduzca. Los exámenes de laboratorio fueron normales. La radiografía de tórax mostró el mediastino ensanchado, un proceso en lóbulo medio con efecto atelectásico, y un nódulo en hemitórax izquierdo. La tomografía computarizada (TC torácica de alta resolución evidenció proceso alveolar derecho en lóbulo medio y un nódulo pulmonar izquierdo calcificado de tipo inespecífico. El estudio inmunohistoquímico de la biopsia pulmonar fue compatible con Linfoma de Células B de la zona marginal extraganglionar del tejido linfoide asociado a mucosa (Linfoma MALT de pulmón. La TC corporal y la Tomografía por Emisión de Positrones (PET evidenciaron importantes hallazgos complementarios para determinar la extensión de la enfermedad. El paciente se trato con quimioterapia y actualmente se encuentra en buenas condiciones, sin recidiva de la sintomatología. Dado lo infrecuente de la patología se presenta este caso y se hace una revisión de la literatura

  17. Granulosa Cell Tumor Juvenile - Case Report-Primary Care Tumor de Celulas da Granulosa Juvenil - Relato de Caso em Atenção Primária

    Directory of Open Access Journals (Sweden)

    Anelise Olmos Grings

    2010-11-01

    Full Text Available

    The authors given an account of children of 2 years and 4 months with ginaecology complaints (white vaginal secretion, diffuse abdominal pain and early pseudo-puberty. Beginning investigation in Unity of Health Care PSF of Porto Alegre, are refer to the secondary levei was diagnosis ovarian tumor (Juvenile Granulosa Cell Tumor Ovary.

    Os autores relatam o caso de urna criança de 2 anos e 4 meses com queixas ginecológicas (secreção vaginal branca, dor abdominal difusa e pseudopuberdade precoce (telarca. Iniciada investigação em Unidade de Saúde PSF de Porto Alegre, sendo referenciada ao nível secundário onde foi diagnosticada neoplasia ovariana (Tumor de Células da Granulosa Juvenil.

  18. Photovoltaic network connection portraits (XIV): The issue of hot cells; Retraso de la conexion fotovoltaica a la red (XIV): El asunto de las celulas calientes

    Energy Technology Data Exchange (ETDEWEB)

    Lorenzo, E.; Martinez, F.; Moreton, R.

    2009-07-01

    In some cases due to plants performance is not as correct as hoped, and in some other, simply because the nice colors of the thermographs resulted very attractive to plenty of people; many photovoltaic generators have been profusely thermography within the current year. It has caused a special interest on hot cells phenomenon, that is to say those cells which operate at a significantly higher temperature than other placed in the same panel. (Author) 4 refs.

  19. Dancoff factors of unit cells in cluster geometry with partial absorption of neutrons; Fatores de Dancoff de celulas unitarias em geometria cluster com absorcao parcial de neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Leticia Jenisch

    2011-01-15

    In its classical formulation, the Dancoff factor for a perfectly absorbing fuel rod is defined as the relative reduction in the incurrent of resonance neutrons into the rod in the presence of neighboring rods, as compared to the incurrent into a single fuel rod immersed in an infinite moderator. Alternatively, this factor can be viewed as the probability that a neutron emerging from the surface of a fuel rod will enter another fuel rod without any collision in the moderator or cladding. For perfectly absorbing fuel these definitions are equivalent. In the last years, several works appeared in literature reporting improvements in the calculation of Dancoff factors, using both the classical and the collision probability definitions. In this work, we step further reporting Dancoff factors for perfectly absorbing (Black) and partially absorbing (Grey) fuel rods calculated by the collision probability method, in cluster cells with square outer boundaries. In order to validate the results, comparisons are made with the equivalent cylindricalized cell in hypothetical test cases. The calculation is performed considering specularly reflecting boundary conditions, for the square lattice, and diffusive reflecting boundary conditions, for the cylindrical geometry. The results show the expected asymptotic behavior of the solution with increasing cell sizes. In addition, Dancoff factors are computed for the Canadian cells CANDU-37 and CANFLEX by the Monte Carlo and Direct methods. Finally, the effective multiplication factors, k{sub eff}, for these cells (cluster cell with square outer boundaries and the equivalent cylindricalized cell) are also computed, and the differences reported for the cases using the perfect and partial absorption assumptions. (author)

  20. Technological study of experimental proton exchange polymer membrane fuel cells; Estudo tecnologico de celulas a combustivel experimentais a membrana polimerica trocadora de protons

    Energy Technology Data Exchange (ETDEWEB)

    Santoro, Thais Aranha de Barros

    2004-07-01

    Experimental studies to achieve an optimized behavior of a unit PEM-fuel cell with an active area of 25cm{sup 2} were carried out. Polarization curves, surface response methodology and regression methodology were used for the analysis. The different methodologies identified the interactions between the parameters that were studied. These parameters were, humidifying temperature, fuel cell operating temperature and the flows of hydrogen and oxygen. MEAs were produced by the spray and hot pressing hybrid method, developed at IPEN. The studies were done with these MEAs and equivalent commercial ones. The MEAs producing method used induced an important variation on the quantity of platinum in the electrodes. This fact has showed to have a great influence in the results. The optimized values were: oxygen flow from 30 to 35% of consume (70 to 65% of excess), fuel cell operating temperature from 60 to 62 deg C and 0.41 mg of platinum in the electrodes. The hydrogen flows and the humidifying temperature have not showed a major influence in the fuel cell behavior, in the studied variable range. (author)

  1. Conductor polymeric membranes with potential for application in PEM type fuel cells; Membranas polimericas condutoras com potencialidades para aplicacao em celulas a combustivel do tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Brioude, Michel de Meireles; Sodre, Livia Farias; Boaventura Filho, Jaime Soares; Jose, Nadia Mamede [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil)

    2006-07-01

    In this work two series of membranes were prepared; they were based on hybrid organic-inorganic or composite materials and presented potentiality for application in Proton Exchange Membrane Fuel Cell, PEMFC. The polymeric phase was constituted of poly(dimethylsiloxane), PDMS, crosslinked with tetra ethoxysilane, TEOS, with a 70%/30% ratio. Phosphotungstic acid (PWA) or the sodium monododecylsulphate (MDS), as proton conductors, were added to the inorganic network, close to the gel point; the mixtures were transferred to a cast. The films were characterized by infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The membranes showed good properties, as flexibility, thermal and mechanical stability with potentiality to be used as conducting membranes in technological applications. (author)

  2. Analise da expressão genica das celulas mononucleares de sangue de cordão umbilical humano : implicação no ciclo celular

    OpenAIRE

    Angela Cristina Malheiros Luzo

    2005-01-01

    Resumo: A reconstituição da linhagem hematopoiética é um procedimento comum no tratamento de várias doenças hematológicas e não hematológicas. A medula óssea tem sido a principal fonte de obtenção de células tronco nos últimos 50 anos. Recentemente, células tronco obtidas de sangue periférico pós-mobilização e células mononucleares de sangue de cordão umbilical humano têm sido utilizadas com sucesso. Entretanto, as células tronco parecem ter características diferentes. Vários estudos foram pu...

  3. Polypropylene (PP) based proton exchange membrane for use in fuel cell; Obtencao de membrana trocadora de protons para uso em celula a combustivel, a base de polipropileno (PP)

    Energy Technology Data Exchange (ETDEWEB)

    Zen, Heloisa Augusto

    2008-07-01

    The radiation-induced graft of styrene onto polypropylene (PP) films was carried out by simultaneous irradiation method in a cobalt-60 source. The PP films were immersed in a solution of styrene with toluene (1:1 and 4:1, v/v) under inert atmosphere and at room temperature and then submitted at 20, 40, 80 and 100 kGy. After graft reaction the films were kept at room temperature under inert atmosphere for periods of 7, 14, 21 and 28 days in order to evaluate the degree of grafting. At the end of each period the films were sulfonated to provide the hydrophilic property to PP. The degree of grafting (DOG) was gravimetric determined and the chemical changes in the grafted and sulfonated films were characterized by Infrared Spectroscopy, Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry and the Ion Exchange Capacity (IEC) was calculated. The greatest DOG was obtained after 21 days of post-irradiation. By TGA the grafted films exhibited a decrease in the thermal stability, while the sulfonated exhibited an increase. By DSC was possible to verify that matrix polymeric did not suffer any drastic change in the melting temperature after grafting and sulfonation reactions. The IEC calculated shows that the new membrane developed has ionic conductivity property. (author)

  4. Photovoltaic cells using blends of poly aniline-DBSA and EPDM; Celulas fotovoltaicas utilizando blendas de polianilina-DBSA e EPDM

    Energy Technology Data Exchange (ETDEWEB)

    Carinhana Junior, Dermeval; Faez, Roselena; Paoli, Marco-Aurelio de [Universidade Estadual de Campinas, SP (Brazil). Inst. de Quimica]. E-mail: derms@iqm.unicamp.br

    1999-07-01

    This paper reports the construction of totally solid photoelectrochemical devices using the PANI-DBSA/EPDM blend and a polymeric electrolyte. The blend presents itself as a photoactive material that can be obtained in a large scale although the observed low efficiency.

  5. Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação

    OpenAIRE

    Alexandre Donizeti Martins Cavagis

    2005-01-01

    Resumo: O objetivo central deste trabalho foi avaliar a modulação de MAPKs em células de leucemia humana induzidas a apoptose e diferenciação. No capítulo 1, estudou-se o efeito da tetrahidroxiquinona (THQ), uma molécula que pode participar de um ciclo redox formando radicais semiquinona e, conseqüentemente, espécies reativas de oxigênio (EROs). A citotoxicidade da THQ, mediada pela formação de EROs, foi revertida por tratamento das células com glutationa e N-acetil-L-cisteína. A produção de ...

  6. An approximated method of calculation of neutron spectra in reactor cells; Um metodo aproximado de calculo do espectro de neutrons em celulas de reatores

    Energy Technology Data Exchange (ETDEWEB)

    Caldeira, Alexandre D. [Centro Tecnico Aeroespacial (CTA-IEAv), Sao Jose dos Campos, SP (Brazil). Inst. de Estudos Avancados

    2000-07-01

    This work deals with the cell neutron spectra calculated with the transport equation for an infinite medium applied to the homogenized cell. Considering a radioisotope production reactor fuel cell, as a sample case, the maximum deviation found between the approximated and the S{sub N} methods was 13%. (author)

  7. Giant cell tumor of the metatarsal bone: case report and review of the literature; Tumor de celulas gigantes do metatarso: relato de caso e revisao da literatura

    Energy Technology Data Exchange (ETDEWEB)

    Benites Filho, Paulo R.; Escuissato, Dante L. [Hospital de Clinicas da Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Servico de Radiologia]. E-mail: danteluiz@onda.com.br; Urban, Linei A.B.D. [DAPI - Diagnostico Avancado por Imagem, Curitiba, PR (Brazil); Gasparetto, Taisa P. Davaus [Hospital Universitario Antonio Pedro (HUAP), Niteroi, RJ (Brazil). Dept. de Radiologia; Sakamoto, Danielle; Ioshii, Sergio [Hospital de Clinicas da Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Servico de Anatomia Patologica; Marchiori, Edson [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia

    2007-07-01

    Giant cell tumor of bone is a rare neoplasm and account for 5% of all primary bone tumors. It is common in the knee and wrist, but rare in the small bones of the foot. The authors report a 32-year old male patient presented with a four-month history of right foot pain. Plain radiographs showed an expansive lytic lesion involving the first right metatarsal bone. Computed tomography scan demonstrated a radiolucent lesion with well-defined borders. Biopsy was performed and the histological diagnostic was giant cell tumor. The authors emphasize the correlation between the imaging and histological findings. (author)

  8. Desmoplastic small round cell tumor: a case report and review of the literature; Tumor desmoplastico de pequenas celulas: relato de caso e revisao da literatura

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Luiz Gustavo Teixeira [Hospital e Maternidade Marieta Konder Bornhausen, Itajai, SC (Brazil). Servico de Medicina Interna; Isberner, Rony Klaus [Hospital e Maternidade Marieta Konder Bornhausen, Itajai, SC (Brazil). Servico de Radiologia]. E-mail: ronyklaus@terra.com.br; Scolaro, Bruno Lorenzo [Universidade do Vale do Itajai, SC (Brazil). Curso de Medicina]. E-mail: brunoscolaro@yahoo.com.br; Sezerino, Daniel Oseias [Universidade do Vale do Itajai, SC (Brazil). Curso de Medicina. Disciplina de Anatomia Patologica; Almeida, Daniel da Silva [Hospital e Maternidade Marieta Konder Bornhausen, Itajai, SC (Brazil). Servico de Radiologia e Diagnostico por Imagem

    2006-10-15

    Desmoplastic small round cell tumor is a rare and highly aggressive neoplasm that predominantly occurs in young adult males. Pain and abdominal distention are frequent, ascites could be observed. His histogenesis is uncertain and the most common location is the peritoneal cavity. This tumor is characterized by presenting a distinct morphology and polyphenotypic differentiation. We present in this report the case of an adolescent admitted in the service of Internal Medicine and Radiology of the Hospital and Maternity Marieta Konder Bornhausen, Itajai, SC, Brazil, in year 2005. (author)

  9. Direct methanol fuel cells: Pt-Ni/C binary electrocatalysts; Celulas a combutivel de metanol direto: eletrocatalisadores binarios de Pt-Ni/C

    Energy Technology Data Exchange (ETDEWEB)

    Salgado, Jose Ricardo Cezar; Antolini, Ermete; Santos, Ana Maria dos; Gonzalez, Ernesto Rafael [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Inst. de Quimica], e-mail: salgado@iqsc.usp.br

    2004-07-01

    Direct methanol fuel cells use platinum alloys as more efficient catalysts than platinum. In the case of binary alloys, the second metal affects several properties of platinum, like the interatomic distance, the electronic density and the capacity of forming oxygenated species at lower potentials. In this work, Pt-Ni catalysts supported on high surface area carbon (Pt-Ni/C) were prepared and characterized, and tested as catalysts in the anode and the cathode in direct methanol fuel cells. In both cases the performance of the material was better than that of Pt/C, and comparing the two situations it was better when the material was used in the cathode. The improved performance in the cathode was attributed to the nickel that forms a true alloy with platinum, while the better performance in the anode was attributed to the presence of nickel oxides. (author)

  10. Interpenetrating polymer network membranes for fuel cells: infrared vibrational spectroscopy; Membranes baseadas dm redes polimericas interpenetrantes para celulas a combustivel: estudo por espectroscopia vibracional no infravermelho

    Energy Technology Data Exchange (ETDEWEB)

    Loureiro, Felipe A.M.; Rocco, Ana Maria [Grupo de Materiais Condutores e Energia, Escola de Quimica, Universidade Federal do Rio de Janeiro, RJ (Brazil)], e-mail: amrocco@eq.ufrj.br; Pereira, Robson Pacheco [Instituto de Ciencias Exatas, Universidade Federal Fluminense (UFF), Volta Redonda, RJ (Brazil)

    2011-07-01

    In the present work, proton conductive membranes based on IPN matrices doped with H{sub 3}PO{sub 4} were developed. The characterization by infrared vibrational spectroscopy evidenced the polymerization of DGEBA and the immobilization of PEI chains, originating a structure containing basic sites suitable for proton coordination and conduction. The FTIR characterization evidenced the polymerization of DGEBA in the presence of PEI thus forming Semi-IPN membranes which, after doped with H{sub 3}PO{sub 4}, exhibited conductivity values of 10{sup -4} W{sup -1}cm{sup -1} at room temperature and 10{sup -3} {omega}{sup -1}cm{sup -1} at 80 degree C, as well as a dependency of conductivity with temperature following the Arrhenius model. The activation energy values (14,33 and 12,96 kJ.mol{sup -1}) indicated a proton conduction mechanism predominantly vehicular in the matrices studied under 100% relative humidity. (author)

  11. Snake venoms components with antitumor activity in murine melanoma cells; Componentes derivados de venenos de serpentes com acao antitumoral em celulas de melanoma murino

    Energy Technology Data Exchange (ETDEWEB)

    Queiroz, Rodrigo Guimaraes

    2012-07-01

    Despite the constant advances in the treatment of cancer, this disease remains one of the main causes of mortality worldwide. So, the development of new treatment modalities is imperative. Snake venom causes a variety of biological effects because they constitute a complex mixture of substances as disintegrins, proteases (serine and metalo), phospholipases A2, L-amino acid oxidases and others. The goal of the present work is to evaluate a anti-tumor activity of some snake venoms fractions. There are several studies of components derived from snake venoms with this kind of activity. After fractionation of snake venoms of the families Viperidae and Elapidae, the fractions were assayed towards murine melanoma cell line B16-F10 and fibroblasts L929. The results showed that the fractions of venom of the snake Notechis ater niger had higher specificity and potential antitumor activity on B16-F10 cell line than the other studied venoms. Since the components of this venom are not explored yet coupled with the potential activity showed in this work, we decided to choose this venom to develop further studies. The cytotoxic fractions were evaluated to identify and characterize the components that showed antitumoral activity. Western blot assays and zymography suggests that these proteins do not belong to the class of metallo and serine proteinases. (author)

  12. EFECTO DEL TIPO Y CONCENTRACION DE LA FUENTE DE CARBONO SOBRE LA SINTESIS Y GLICOSILACION DE TPA PRODUCIDO EN CELULAS CHO

    OpenAIRE

    Berrios, Julio

    2008-01-01

    Los cultivos de células animales han sido ampliamente usados en el último tiempo para la producción de proteínas con uso terapéutico, debido a su habilidad de realizar modificaciones post-traduccionales, incluyendo la glicosilación. La glicosilación de la proteína ha mostrado ser esencial para conseguir la actividad biológica deseada. Sin embargo, dada la alta complejidad de estos sistemas, el conocimiento acerca de los procesos metabólicos y los mecanismos que afectan la producción es aún...

  13. Localized giant cell tumors in the spinal column radiologic presentation. Tumor de celulas gigantes localizado en la columna dorsal: presentacion radiologica poco frecuente

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Echeverria, M.A.; Parra Blanco, J.A.; Pagola Serrano, M.A.; Mellado Santos, J.M.; Bueno Lopez, J.; Gonzalez Tutor, A. (Hospital Marques Valdecilla. Santander (Spain))

    1994-01-01

    Given the uncommonness of the location of giant cell tumors (GCT) in the spinal column and the limited number of studies published, we present a case of GCT located in the spinal column, which involved both vertebral bodies and partially destroyed the adjacent rib. (Author)

  14. Polyethylene (PE) based proton exchange membrane for use in fuel cell; Obtencao de membranas trocadoras de protons a base de polietileno para uso em celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Moraes, Gilberto de Oliveira

    2008-07-01

    The irradiation - induced graft of styrene onto polyethylene (PE) in a Cobalt-60 source was carried out using direct (simultaneous) and indirect (preirradiation and peroxidation) methods at room temperature. The dose applied in both cases varied from 0,5 to 80 kGy; In the direct method, the films were immersed in a solution of styrene: methanol (30:70 v/v) and 30% of sulfuric acid(additive) in glass ampoules of 40 ml under inert atmosphere, and then irradiated. After irradiation process, the samples were kept in solution for 8 hours and taken off the ampoules, dried in oven at 60 deg C for another 8 hour period until constant weight. In pre-irradiation method, the samples were irradiated in dry and sealed ampoules, under inert atmosphere. The solution was then added to the samples, and after 8 hours, taken off, dried, sealed and weighted. In peroxidation method, the difference was not inert atmosphere but atmosphere of air (0{sub 2}).For each samples it was calculated the degree of grafting (DOG). The samples that showed some DOG were sulfonated, characterized (DSC, TGA and infrared) and the ion exchange capacity (IEC) was calculated. The samples processed by indirect method presented no DOG at all. The samples processed by direct method present grafting (best result was 80 kGy). These samples, after sulfonated, presented ion exchange properties. (author)

  15. Manganates of lanthanum and strontium as cathode of fuel cells (SOFC); Manganatos de lantanio e estroncio como catodo de celulas combustiveis (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Marfran C. de; Nascimento, Rubens M. do; Martinelli, Antonio E.; Melo, Dulce M.A. [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2004-07-01

    The fuel cells if constitute currently as a promising alternative modular generation of electric energy to leave of chemical energy. The SOFCs is distinguished as promising for the industry of the oil, therefore they can use the produced natural gas in the platforms as combustible, allowing generation of raised power electric.The material of cathode constituent of the cell are oxides with perovskites structure, normally doped with a earthy-alkaline element (Sr{sup +3}). In this work, two compositions of the La{sub 1-x}Sr{sub x} MnO{sub 3} system were synthesized (x = 0,15 and 0,30), through the Pechini method and after resultants of the process they were characterized by X- Ray diffraction, Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG/DTG) and laser grain size analysis. The systems in study shown similar behavior how much to the results of absorption in the region of infra-red ray and TG/DTG, therefore if it can prove the loss of organic substance with the increase of the temperature.The average particle diameter for the two systems also increased gradually with the temperature. In general way the synthesis method was satisfactory for the formation of the perovskites phase in the two studied compositions. (author)

  16. Electric power generation using photovoltaic solar cells for low income rural population; Geracao de energia eletrica com celula solar fotovoltaica para populacao rural de baixa renda

    Energy Technology Data Exchange (ETDEWEB)

    Gastaldi, Andre Fava; Souza, Teofilo Miguel de; Mesquita, Rafael Pimenta [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Guaratingueta, SP (Brazil). Centro de Energias Renovaveis], e-mail: teofilo@feg.unesp.br

    2004-07-01

    With the growing electric energy use demand and almost not expansion of the energy mesh (basically composed by hydroelectric plants) existing in the country, several others methods of alternative energy generation may be necessary. Beyond that, the usually employed energy is becoming much more costly, rarer and politically more impracticable like burn fuels as oil and coal. The use of renewable approaches of energy, that are 'infinite' energies (as the wind and the light of the sun, for example), can become an excellent alternative. In this new energy group, the solar energy transformed by the use of photo voltage cells is becoming very important. The 'clear' solar radiation tends to be a more intelligent and practical option, and the future technology of energy storage will be able to solve the problem of the regions that have less sunny days. Its main advantages on the other alternative sources of energy are the trustworthiness and the previsibility. Its biggest disadvantage consists on the fact that technical limitations can not allow a solar energy generator to distribute electricity for a city. It is interesting to notice that with the development of projects as this in alternative energy, isolated areas that does not access electricity distribution network (as some far regions), it has become the most viable option of generation of electric energy. Another point is that even with the distribution network it has to be checked if it is possible to use this electricity consulting the company credential that work at those localities of consumption. Moreover, many regions of the country already installed the solar energy system for water heating, confirming that, the existing structure allows the installation of a a solar cells generation energy system without many problems. In this project, we introduce a method for electric energy generation by solar cells for rural population of low gains. This option uses low cost materials but with a good quality level. Also presents a comparative table showing a kit for rural areas employment which is constituted of: 3 fluorescent light bulbs (power of 9 W, 3 hours per day, 7 days per week), 1 T.V. of 14' ' (power of 15 W, 3 hours per day, 7 days per week) and 1 Radio (power of 5 W, 6 hours per day, 5 days per week). (author)

  17. Infiltración meningea por leucemia a celulas plasmáticas y por linfoma no Hodgkin: estudio citomorfologico en el liquido cefalo-raquideo

    Directory of Open Access Journals (Sweden)

    Arnaldo Tabares Olives

    1982-06-01

    Full Text Available Son presentadas las características citomorfológicas anormales encontradas en el LCR de dos pacientes, uno de ellos sufriendo de leucemia aguda a células plasmáticas y otro de linfoma mixto no Hodgkin. Se enfatiza la importancia del estudio del LCR en estas patologias.

  18. EFECTO DE LA SOBRECARGA DE HIERRO SOBRE LA ACTIVACION DE INF-KB EN CELULAS DE ENDOMETRIO HUMANO COMO POSIBLE MECANISMO ASOCIADO AL A PATOGENESIS DE LA ENDOMETRIOSIS

    OpenAIRE

    ALVARADO DIAZ, CARLOS PATRICIO

    2014-01-01

    La endometriosis es una enfermedad en la cual el tejido endometrial crece fuera de la cavidad uterina. El establecimiento de lesiones endometriósicas peritoneales se originaría a partir de células endometriales refluidas a través de las trompas uterinas durante la menstruación. Asociado al desarrollo de la enfermedad se encuentra un ambiente intraperitoneal pro-inflamatorio. Se ha visto que el factor de transcripción pro-inflamatorio nucelar factr-kappaB (NF-KB), en particular el dímero de NF...

  19. Operating Cell Temperature Determination in Flat-Plate Photovoltaic Modules; Calculo de la Temperature de Operacion de Celulas Solares en un Panel Fotovoltaico Plano

    Energy Technology Data Exchange (ETDEWEB)

    Chenlo, F.

    2002-07-01

    Two procedures (simplified and complete) to determine the operating cell temperature in photovoltaic modules operating in real conditions assuming isothermal stationary modules are presented in this work. Some examples are included that show the dependence of this temperature on several environment (sky, ground and ambient temperatures, solar irradiance, wind speed, etc.) and structural (module geometry and size, encapsulating materials, antirreflexive optical coatings, etc) factors and also on electrical module performance. In a further step temperature profiles for non-isothermal modules are analysed besides transitory effects due to variable irradiance and wind gusts. (Author)

  20. Fabrication and tests of anode supported solid oxide fuel cell; Fabricacao e testes de celula a combustivel de oxido solido suportada no anodo

    Energy Technology Data Exchange (ETDEWEB)

    Florio, D.Z. de [UNESP, Araraquara, SP (Brazil)], e-mail: dzflorio@ipen.br; Fonseca, F.C.; Franca, Y.V.; Muccillo, E.N.S.; Muccillo, R. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Berton, M.A.C.; Garcia, C.M. [LACTEC - Instituto de Tecnologia para o Desenvolvimento, Curitiba, PR (Brazil)

    2006-07-01

    A laboratory setup was designed and put into operation for the development of solid oxide fuel cells (SOFCs). Ceramic single cells were fabricated by low-cost methods, and emphasis was given to the use of ready available raw materials. The whole project consisted of the preparation of the component materials - anode, cathode, and electrolyte - and the buildup of a hydrogen leaking-free sample chamber with platinum leads and current collectors for measuring the electrochemical properties of single SOFCs. Anode-supported single SOFCs of the type (ZrO{sub 2}:Y{sub 2}O{sub 3} + NiO) anode / (ZrO{sub 2}:Y{sub 2}O{sub 3}) electrolyte / (La{sub 0.65}Sr{sub 0.35}MnO{sub 3} + ZrO{sub 2}:Y{sub 2}O{sub 3}) cathode have been prepared and tested at 700 deg C and 800 deg C after in situ H{sub 2} anode reduction. The main results show that the slurry coating method resulted in single-cells with good reproducibility and reasonable performance, suggesting that this method can be considered for fabrication of SOFCs. (author)

  1. LA ENZIMA OCRL1 Y LAS PROTEINAS ENDOSOMALES, APPL1 Y WDFY2, PARTICIPAN EN EL TRAFICO DE MEGALINA EN CELULAS EPITELIALES RENALES

    OpenAIRE

    SANDOVAL GALARCE, LISETTE PAMELA

    2013-01-01

    Megalina es un receptor endocítico miembro de la familia de receptores de lipoproteínas de baja densidad (LDLR). Esta proteína se localiza en la superficie apical de varios epitelios y se expresa de manera importante en el epitelio de túbulo proximal renal. La principal función de megalina en el epitelio renal es la reabsorción de proteínas de bajo peso molecular desde el filtrado glomerular. Los ligandos de megalina incluyen calcio, albumina, insulina, leptina, hormona paratiroídea, angio...

  2. Intraabdominal desmoplastic small cell tumor: radiological aspects - a case report; Tumor de pequenas celulas desmoplasico intra-abdominal - relato de um caso

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Leonardo Velloso; Siciliano, Antonio Alexandre O.; D' Annunciacao, Gisele Esteves; Nunes, Vania Cristina S.R. [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Dept. de Radiologia; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia

    1999-06-01

    The authors describe a case of a 21-year-old white female admitted with abdominal pain and distension, as well as bowel movement alteration. A hard, lobulated and painful abdominal mass was found at physical exam and signs of ascitis were also presented. Abdominal and pelvic ultrasound and computed tomography demonstrated and heterogeneous mass, adhering to the anterior abdominal wall, extending from the umbilicus to the pelvis and displacing the urinary bladder inferiorly and bowel loops posteriorly. Cul-the-sac implants were also noted. The major omentum was diffusely infiltrated by nodules as laparotomy. A desmoplastic small cell tumor was found after histopathological analysis of the surgical specimen. (author)

  3. Cogeneration, micro turbines and fuel cells: perspectives for distributed generation in Brazil; Cogeracao, microturbinas e celulas a combustivel: perspectivas para geracao distribuida no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Leite, Marco Antonio Haikal [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES)

    2004-07-01

    Brazil has a large potential to install distributed generation systems, using natural gas or renewable like solar, wind or biomass energy. Regarding urban centers, natural gas fired cogeneration and other distributed energy technologies find economical applications. Cogeneration is defined as the generation of two kinds of useful energy from a single energy source. Usually, electrical energy and thermal energy as steam or hot water are produced. By using the absorption refrigeration cycle, chilled water can also be produced to be used in air conditioned systems, often called tri generation, a good alternative to industries, commercial buildings, shopping centers, hospitals, schools and universities. Micro turbines find utilization whenever natural gas is available, but not electricity, like gas compression installations, unmanned platforms or remote production fields. Fuel cells are used in systems requiring high levels of reliability or wherever the non availability cost is high. This paper describe technical and economical data related to PETROBRAS Research Center (CENPES) 3,200 kW electric energy and 1,000 RT chilled water cogeneration system, 200 kW fuel cell and 30 kW and 60 kW microturbines. (author)

  4. EFECTOS DE 2-METOXIESTRADIOL SOBRE LOS NIVELES DEL FACTOR TISULAR Y EL POTENCIAL INVASIVO DE LAS CELULAS DE CANCER DE MAMA

    OpenAIRE

    QUEZADA BRITO, MARISOL

    2009-01-01

    El 2-metoxiestradiol (2ME) es un metabolito del 17¡3-estradiol que ha emergido como un potencial agente para el tratamiento del cáncer, ya que inhibe la proliferación y suprime el crecimiento de tumores. Aunque se han propuesto varios blancos moleculares para explicar sus efectos, su mecanismo de acción aún es incierto. El estado hipercoagulante y la metástasis del tumor primario hacia los órganos vitales son las principales causas de muerte de los pacientes con cáncer. Se ha postulado que...

  5. Platinum catalysts recovery of the proton exchange membrane fuel cell; Recuperacao de catalisadores de platina da celula a combustibel de membrana polimerica trocadora de protons

    Energy Technology Data Exchange (ETDEWEB)

    Fukurozaki, S.H.; Seo, E.S.M. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Ciencia e Tecnologia de Materiais. Lab. de Processamento de Residuos

    2006-07-01

    Currently, platinum is the most feasible catalyst for the Proton Exchange Membrane Fuel Cells - PEMFC. Along with platinum's significant importance in this energy system are the high cost of this noble metal and its detrimental effects on the environment. Therefore, recycling this material seems as an alternative to decrease its impacts on the environment and, at the same time, to provide a reduction of the system's costs. A search was conducted for literature and studies about platinum recycling methods. However, only two techniques of platinum recovery, which are still in development, were found. In face of this situation, a recovery method of platinum from deactivated Membrane Electrode Assembly - MEA's was developed, with attention to aspects related to the environment and the necessary requirements for its primary recycling. The results found showed a high recovery ratio and a possibility to reintroduce this metal into the production cycle. (author)

  6. Implementation of a photovoltaic/electrolyzer/fuel cell autonomous system; Implementacao de um sistema autonomo fotovoltaico/eletrolisador/celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Ennio Peres da; Apolinario, Fernando Rezende; Furlan, Andre Luis [Universidade Estadual de Campinas (IFGW/UNICAMP), SP (Brazil). Inst. de Fisica Gleb Wataghin. Lab. de Hidrogenio], Emails: lh2ennio@ifi.unicamp.br, rezende@ifi.unicamp.br; Souza, Rubem Cesar Rodrigues [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Centro de Desenvolvimento Energetico Amazonico], Email: rubem_souza@yahoo.com.br; Pinto, Adailton de Souza [Centrais Eletricas do Norte do Brasil S.A. (ELETRONORTE), Brasilia, DF (Brazil)], Email: adailton@eln.gov.br

    2006-07-01

    This article presents a project whose main objective is to analyze the technical feasibility of using a system based on the production of hydrogen (H{sub 2}) for storing the energy generated for photovoltaic systems. The work involves the design, physical implementation and the performance evaluation of a system for hydrogen electrolytic generation with solar-photovoltaic energy, the treatment os gas, its storage and it use in fuel cell systems. This work will be performed in cooperation between researchers from the Laboratorio de Hidrogenio (LH2) of the UNICAMP and of the Centro de Desenvolvimento Energetico Amazonico (CDEAM) of the UFAM, and is financing by ELETRONORTE.

  7. Development of perovskite cathodes for solid oxide fuel cells (SOFC); Desenvolvimento de catodos de perovskitas para celula a combustivel solido de eletrolito solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Perez, J. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica], e-mail: joelma@iq.unesp.br; Pereira, J.T.; Saeki, M.J. [UNESP, Bauru, SP (Brazil). Faculdade de Ciencias

    2006-07-01

    Solid Oxide Fuel Cells (SOFC) are energy conversion systems of great interest for industrial applications because they present a high efficiency for energy generation and several advantages for the environment. In this work, perovskite type oxides La{sub 085}Sr{sub 0,15}MnO{sub 3}, La{sub 0,7} Sr{sub 0,3}MnO{sub 3}, La{sub 0,6}Sr{sub 0,4}MnO{sub 3}, La{sub 0,85}Sr{sub 0,15}CoO{sub 3}, La{sub 0,7}Sr{sub 0,3}CoO{sub 3}, La{sub 0,6}Sr{sub 0,4}CoO{sub 3}, La{sub 0.6}Sr{sub 0,4}Fe{sub 0,8}Co{sub 0,2}O{sub 3} e La{sub 0.6}Sr{sub 0,4}Fe{sub 0,4}Co{sub 0,6}O{sub 3} were prepared by a polymeric method with the purpose of using them as cathodes in SOFCs. The electrochemical cell was mounted utilizing YSZ (ZrO{sub 2} - 8 mol%Y{sub 2}O{sub 3}) disks as electrolyte, where a paste containing Pt was calcined onto one face while the other one was covered with the oxide materials synthesized ('screen printing'). The oxide materials prepared were characterized by X-ray diffraction, transmission electronic microscopy and thermogravimetry. The oxygen reduction reaction was studied by taking polarization curves in oxygen and/or air (800 deg C a 950 deg C). The best performance was obtained for 15 {mu}m thickness electrodes La{sub 0.6}Sr{sub 0,4}MnO{sub 3} and La{sub 0.6}Sr{sub 0,4}MnO{sub 3} with addition of dispersed Pt. (author)

  8. Modelling and essay or the polarization curve of a polymeric membrane fuel cell; Modelagem e ensaio da curva de polarizacao de uma celula a combustivel de membrana polimerica

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Silvio Carlos Anibal de; Xavier, Bruno Domont; Gatti, George Cassani; Ribeiro, Rodrigo Minguita [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia (COPPE). Programa de Engenharia Mecanica]. E-mails: silvioa@gmail.com; brunodomont@gmail.com; gatti_ufrj@yahoo.com.br; rminguita@yahoo.com.br; Furtado, Jose Geraldo de Melo [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil). Dept. de Tecnologias Especiais]. E-mail: furtado@cepel.br

    2006-07-01

    This paper describes the essays performed with a polymeric membrane fuel cell (PEMFC) at the test laboratories of the ELETROBRAS Electric Energy Research Center (CEPEL/ELETROBRAS) manufactured by the Eletrocell, which allows to study the influence of some functional parameters (voltage, current, mass and pressure fluxes)

  9. Synthesis, characterization and electrical properties of solid electrolyte for solid oxide fuel cell; Preparacao, caracterizacao e propriedades eletricas de eletrolito solido para celula a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Berton, Marco Antonio Coelho; Garcia, Carlos Mario; Matos, Jeferson Hrenechen [Instituto de Tecnologia para o Desenvolvimento (LACTEC), Curitiba, PR (Brazil)], Emails: felsky@latec.org.br, garcia@latec.org.br, jeferson.h@latec.org.br

    2010-04-15

    Solid electrolytes of BaCe{sub 08}Gd{sub O29} were prepared by the polymeric precursor method. X-ray diffraction data shows a single phase with orthorhombic crystalline structure. The densification process was followed by scanning electronic microscopy and apparent density measurements. The apparent density was developed for different temperatures of sintering, reaching > 96% for sintered temperature of 1550 {sup 0}C deg . The electrochemical impedance analysis was development in the temperature of 400-700 deg C, in air atmosphere at 700 deg C a value of 30,6 mS.cm{sup -1} was obtained. The results of conductivity have confirmed the gadolinium doped barium cerate has a great potential for use as solid electrolyte for intermediate temperature solid oxide fuel cell, at experimental controlled conditions. (author)

  10. Wind, photovoltaic and fuel cell energy for communication stations; Energia eolica, fotovoltaica e de celula a combustivel para estacoes de comunicacao

    Energy Technology Data Exchange (ETDEWEB)

    Sousa, Iolanda; Pereira, Jose; Alcobia, Hernani [Net Plan Telecomunicacoes e Energia, Lisboa (Portugal); Pereirinha, Paulo [Instituto Politecnico de Coimbra (Portugal); Instituto para Engenharia de Sistemas e Computadores de Coimbra (Portugal)

    2011-10-15

    This paper presents a hybrid system that provides power to a remote and autonomously telecommunications station by means of electrical solar generators, wind and hydrogen fuel cell. In the absence of sufficient sun and wind, a bank of batteries and hydrogen produced on-site guarantee the power supply. The station can still be remote monitored and managed.

  11. Analysis of the thermal and electrical behavior of commercial photovoltaic cells in inverse operation; Analisis del comportamiento termico y electrico de celulas fotovoltaicas comerciales en operacion inversa

    Energy Technology Data Exchange (ETDEWEB)

    Margineda, D.; Alonso-Garcia, M. C.; Sanchez-Friera, P.

    2008-07-01

    In order to analyse the thermal and electrical behaviour of commercial solar cells in reverse operation photovoltaic cells of the same type, but different class (different efficiency in the same type cell) were selected. electrical characterisation of the samples was performed through I-V curve measurements in direct and reverse bias, what gave information about the type of reverse I-V characteristics. besides, infrared characterisation showed temperature distributions along cell surface, pointing out localised heating that could cause hot-spots. From both characterisations (electrical and thermal) it is possible to obtain relations between the class of the cell, the type of reverse characteristics and maximum temperatures in case of partial shading or faulty operation. (Author)

  12. Langerhans cell histiocytosis with multifocal sites. A case report; Histiocitose de celulas de Langerhans com acometimento multifocal. Relato de um caso

    Energy Technology Data Exchange (ETDEWEB)

    Albuquerque, Silvio Cavalcanti; Silveira, Andre; Ribeiro Neto, Joao Vicente [Instituto Materno Infantil de Pernambuco (IMIP), Recife (Brazil)

    1998-05-01

    The authors report a case of Langerhans cell histiocytosis in which the patient, at the time of the diagnosis, had lesions at the central nervous system, mediastinum and long bones, all detectable in simple roentgenogram. The recent reclassification of this disease is discussed together with it`s radiological findings. (author) 4 refs., 6 figs.

  13. CARCINOMA ODONTOGÉNICO DE CELULAS CLARAS. ESTUDIO CLÍNICO, RADIOLÓGICO Y PATOLÓGICO DE UN CASO.

    Directory of Open Access Journals (Sweden)

    Rosaura Rodríguez Flores

    2004-01-01

    Full Text Available Los tumores odontogénicos (TO son un grupo complejo y poco frecuente de neoplasias con características clínicas, radiológicas, histopatológicas y evolución muy variables. El Carcinoma odontogénico de células claras (COCC es una neoplasia maligna, de comportamiento agresivo variable que se presenta en maxilares. Histológicamente se constituye de nidos de células, frecuentemente de citoplasma claro, rico en glucógeno, rodeadas por bandas de colágena. Pertenece al grupo de los tumores odontogénicos epiteliales malignos. Se presenta el caso de un hombre de 57 años con un tumor en hueso y seno maxilar izquierdo, sometido a hemimaxilectomía subtotal izquierda. Los estudios de imagen demostraron una lesión destructiva mixta en maxilar y seno maxilar izquierdo y la histología reveló la imagen clásica de COCC y la inmunohistoquímica mostró inmunoreactividad para Queratina AE1/AE3, EMA y S-100 (focal. Se demostró abudante glucógeno mediante la reacción de PAS en el citoplasma de las células neoplásicas. El paciente ha tenido seguimiento por 5 años y está asintomático después de la cirugía sin tratamiento complementario con radioterapia.

  14. Technological evaluation of fuel cells using natural gas for distributed power generation; Avaliacao tecnologica da utilizacao de gas natural em celulas a combustivel para geracao distribuida de energia

    Energy Technology Data Exchange (ETDEWEB)

    Brandao, Mauricio O. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE). Programa de Engenharia Mecanica; Giannini, Marcio P.; Arouca, Mauricio C. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE). Programa de Planejamento Energetico

    2004-07-01

    The search for sustainable and more rational means of power generation motivates the scientific crew to search for more efficient and cleaner systems. Oil dependence becomes from the kind of development that the humanity had and cannot be dismissed. The question is how to use this source in a more intelligent way. Fuel Cells are electrochemical devices that convert into electric energy the chemical energy from oxi-reduction reactions between a fuel and an oxidant. The current fuel used in a Fuel Cell is hydrogen and oxygen is the oxidant. The great advantage of this device is its efficiency, higher than the one achieved with internal combustion engines. Also Fuel Cells are not limited by Carnot's efficiency. This paper is about the implementation of a distributed generation system using Fuel Cells. Technical aspects are approached together with economical and environmental needs. The already existence of Gas pipelines and the grown production of Natural Gas presented by Brazil turns it into a good market for the implementation of this energy source. The evaluation of this paper shows that is technically possible to use NG in Fuel Cells, mostly in South and Southeast regions, applying the distributed generation of energy concept. The most interesting in a strategic manner is that Brazil already have an indication that it's capable of developing this technology, opening a new market tuning with world's new technological developments. Many research centers develop this technology, not only from the cell composition itself, but also manufacturing techniques. (author)

  15. Synthesis of human prolactin in Chinese hamster ovary (CHO) cells; Sintese de prolactina humana em celulas de ovario de hamster chines (CHO)

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Carlos Roberto Jorge

    2000-07-01

    Three different eukaryotic expression vectors, based on the same selectable gene marker (dhfr), have been used for dhf- CHO cells transfection to rapidly isolate stable cell lines capable of secreting high levels of recombinant human prolactin (rec-hPRL). Two vectors, one codifying a human prolactin (p658-hPRL) and the other a tag-prolactin (p658-tagPRL), contain the complete hepatitis B virus-X (HBV-X) gene coding for a viral transactivator and a sequence derived from the granulocyte-macrophage colony-stimulating factor (GM-CSF) that mediates selective dhfr mRNA degradation. These vectors have the advantage of rapidly obtaining stable cell lines without methotrexate amplification. The highest secretion obtained by these vectors was of approximately 10 {mu}g hPRU10{sup 6} cells/day. The other vector (pEDdc-hPRL) is based on a dicistronic expression system, containing an internal ribosome entry site isolated from the encephalomyocarditis (EMC) virus. This vector before amplification provided secretion levels at least 10 fold lower than that obtained with the other two vectors. However, after three steps of methotrexate amplification, it provided some clones able to secrete up to 30 {mu}g hPRU10{sup 6} cells/day. This is the first report describing the production and purification of rec-hPRL from CHO cells, obtaining secretion levels with both vectors higher than those reported so far for this hormone in other eukaryotic systems. CHO-derived rec-hPRL contained approximately 10 % of the glycosylated form, a value that is consistent with results reported for hPRL purified from the pituitary or from transformed murine C-127 cells. CHO-derived rec-hPRL was purified with good yield, obtaining also a good resolution between non-glycosylated and glycosylated prolactin. The latter, when its potency was determined via an in vitro bioassay, presented a 47 % lower bioactivity. A qualitative and quantitative analysis of these forms was also possible thanks to the setting up of a reversed-phase HPLC technique, for the first time applied to this hormone. A pilot production in a hollow fiber bioreactor provided a highly concentrated medium, though with the presence of considerable amounts of hPRL{sub 11-199} fragments, apparently the result of a proteolytic process. (author)

  16. Pre-surgical staging of renal cell carcinoma using imaging methods; Estadiamento pre-operatorio por imagem do carcinoma de celulas renais

    Energy Technology Data Exchange (ETDEWEB)

    Barroso, Vivian Alvim; Bauab Junior, Tufik [Instituto de Radiodiagnostico Rio Preto, Sao Jose do Rio Preto, SP (Brazil); Barroso Junior, Ubirajara [Universidade Federal de Sao Paulo (UNIFESP), SP (Brazil). Escola Paulista de Medicina. Div. de Urologia

    2000-04-01

    The incidental discovery of renal tumors has become more frequent lately. As a result, a more precise preoperative characterization of such tumors is necessary to allow the selection of the best treatment for each case, such as nepron-sparing surgery, radical nephrectomy, or immunotherapy. Computed tomography has been the most used imaging method to study these tumors. Large tumors, perinephric fat invasion, multicenter tumors, vascular invasion and stage IV are all computed tomography criteria which may exclude nephron-sparing surgery as a curative therapy. Furthermore there are some tomographic pitfalls in the detection of perinephric fat invasion and tumoral nodes. In this paper, the importance and accuracy of computed tomography for renal cancer staging is evaluated. (author)

  17. Stem Cells and Blood: Where have we come from... and where are we going?; Celulas Madre y sangre: De donde venimos y... donde vamos a parar

    Energy Technology Data Exchange (ETDEWEB)

    Bueren, J. A.

    2011-07-01

    Since 1961, they year when the first trial that characterized the behaviour of a stem cell in mice exposed to high doses of radiation was described, research in this field has proceeded at an unpredictable place. Knowledge of the function of hematopoietic stem cells which are responsible for forming blood cells facilitated the development of therapies based on the transplant of bone marrow and other cell source, e. g. blood from the umbilical cord. These breakthroughs, together with the progress of molecular biology and virology, made it possible to manipulate the genome of hematopoietic stem cells so effectively and safely that the transplant of genetically modified cells has become a variable therapeutic alternative for the treatment of certain genetic diseases and also cancer. This brief article describes some of the contributions that our Hematopoiesis and Gene Therapy Division of the CIEMAT and the CIBER for Rare Diseases has been developing in this fascinating field of stem cells and gene therapy, in the context of the international research being carried out in this area. (Author) 34 refs.

  18. Analysis of cogeneration system using fuel cell: cases study; Analise de sistema de cogeracao utilizando celula de combustivel: estudo de casos

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, Jose Luz; Leal, Elisangela Martins [UNESP, Guaratingueta, SP (Brazil). Escola de Engenharia. Dept. de Energia]. E-mails: joseluz@feg.unesp.br; elisange@feg.unesp.br

    2000-07-01

    In this paper, a methodology for the study of a molten carbonate fuel cell cogeneration system associated to an absorption refrigeration system, for the electricity and cold water production, and applied to two establishments, is presented. This system permits the recovery of waste heat, available between 600 deg C e 700 deg C. Initially, some technical information about the most diffusing types of the fuel cell demonstration in the world are presented. In the next step, an energetic, exergetic and economic analysis are carry out, seeking the use of fuel cells, in conditions of prices and interest of Brazil. In conclusion, the fuel cell cogeneration system may have an excellent opportunity to strengthen the decentralized energy production in the Brazilian energy scene. (author)

  19. ESTUDIO DE LA FUNCION DE LA VIA DE SEÑALIZACION SHH/GLI EN LOS PROCESOS DE PROLIFERACION Y DIFERENCIACION DE CELULAS DEL LIGAMENTO PERIODONTAL HUMANO

    OpenAIRE

    MARTINEZ CARDOZO; CONSTANZA

    2011-01-01

    Las células troncales mesenquimales y progenitoras residentes en el ligamento periodontal humano constituyen una población de células troncales adultas con características similares a las células troncales mesenquimales de la medula ósea que son capaces de diferenciarse a varios linajes. Considerando su importante papel en la regeneración periodontal, es de suma importancia la identificación de los factores que controlan su proliferación y diferenciación. Sanie hedgehog (Shh) correspond...

  20. EFECTO DE PMA SOBRE UNA VARIANTE NUCLEAR DE CATEPSINA L Y LA ACTIVACION DEL FACTOR DE TRANSCRIPCION CDP/CUX EN CELULAS CACO-2

    OpenAIRE

    HERMOSILLA AGUAYO, VIVIANA ESTEFANIA

    2012-01-01

    El estudio de los mecanismos moleculares subyacentes al desarrollo del cáncer comprende el uso de compuestos capaces de actuar como promotores tumorales, los que favorecen la proliferación de células incompletamente transformadas. Dentro de ellos, uno de los más ampliamente utilizados es forbol 12-miristato 13-acetato o PMA. Los efectores a nivel celular de este compuesto corresponden a una familia de serina-treonina quinasas, conocidas como "Proteína quinasa C" o PKC, que partici...

  1. Preparation of PVDF and PTFE membranes for fuel cell use; Preparacao de membranas de PVDF e PTFE enxertadas e sulfonadas para uso em celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Geraldes, Adriana N.; Zen, Heloisa A.; Ferreira, Henrique P.; Parra, Duclerc F.; Lugao, Ademar B.; Linardi, Marcelo [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)], e-mail: angeral@ipen.br

    2007-07-01

    Grafting of styrene onto polyvinylidenefluoride (PVDF), polytetrafluoroethylene (PTFE) was studied using styrene/toluene solutions. First, PTFE and PVDF films were prepared and the process was conducted by radiation induced graft polymerization of styrene, by a Co{sup 60} source. Films of PTFE and PVDF were immersed in styrene/toluene at 1:1 (v/v) concentration and then submitted to gamma radiation at 20 kGy doses. After irradiation, the samples were evaluated at the period of 21 days at room temperature in order to observe the grafting degree. Chemical changes in the PVDF and PTFE films after styrene grafting were monitored and the results were evaluated by FTIR, DSC, TGA and degree of grafting (DOG). The ion exchange capacity (IEC) after sulfonation of 1, 2 and 24 hours were also determined. (author)

  2. Radiation therapy for the treatment of feline advanced cutaneous squamous cell carcinoma; A utilizacao da radioterapia no tratamento do carcinoma de celulas escamosas cutaneo felino avancado

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, S.C.S.; Corgozinho, K.B.; Ferreira, A.M.R, E-mail: simonecsc@gmail.com [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil); Carvalho, L.A.V. [Coordenacao dos Programas de Pos-Graduacao em Engenharia (COPPE/UFRJ), RJ (Brazil); Holguin, P.G.

    2014-02-15

    The efficacy of radiation therapy for feline advanced cutaneous squamous cell carcinoma was evaluated. A full course radiation therapy protocol was applied to six cats showing single or multiple facial squamous cell carcinomas, in a total of seven histologically confirmed neoplastic lesions. Of the lesions, one was staged as T{sub 1}, and six as T{sub 4} according to WHO staging system of epidermal tumors. The animals were submitted to twelve radiation fractions of 4 Gy each, on a Monday-Wednesday-Friday schedule, and the equipment used was an orthovoltage unit. Energy used was 120 kV, 15 mA and 2 mm aluminum filter. The cats were evaluated during the treatment and 30 and 60 days after the end of the radiation therapy. In this study, 87% of the lesions had complete remission and 13% partial remission to the treatment. Side effects were considered mild according to Veterinary Radiation Therapy Oncology Group Toxicity criteria, and included erythema, epilation and rhinitis. Radiation Therapy was considered safe for feline cutaneous squamous cell carcinoma, leading to mild side effects and can represent a good therapeutic option. (author)

  3. APOPTOSIS Y REPARACION DEL DAÑO CROMOSOMICO DURANTE G2 EN CELULAS DE PACIENTES CON SINDROME DE NIJMEGEN Y ATAXIA - TELANGIECTASIA

    OpenAIRE

    MARCELAIN CUBILLOS, KATHERINE JENNY

    2003-01-01

    El material genético de las células está expuesto a agentes endógenos y exógenos que causan lesiones en su estructura. La presencia de daño en el DNA, en particular, las fracturas bicatenarias, genero una condición de alta inestabilidad genómica, con el consecuente riesgo de transformación oncogénica. Las células cuentan con sistemas que detectan las lesiones en el DNA, los que desencadenan varios eventos río abajo que en su conjunto se denominan "respuesta al daño en el DNA". ...

  4. Fuel cells using ionic liquids as electrolyte and operating at room temperature; Celulas de combustivel utilizando como eletrolito liquidos ionicos e operando a temperatura ambiente

    Energy Technology Data Exchange (ETDEWEB)

    Botton, Janine Padilha; Souza, Roberto Fernando de; Goncalves, Reinaldo Simoes; Dupont, Jairton [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Inst. de Quimica], e-mail: janine@iq.ufrgs.br

    2004-07-01

    The room temperature imidazolium based ionic liquids, such as 1-n-butyl-3-methylimidazolium tetrafluoroborate (BMI.BF4) are outstanding electrolytes for fuel cells. A 67% overall cell efficiency is achieve using these liquids as supporting electrolytes for a commercially available alkaline fuel cell (AFC) at room temperature operating with air and hydrogen at atmospheric pressure. (author)

  5. The utilization of natural gas in the electricity production through fuel cell; A utilizacao do gas natural na geracao distribuida atraves de celulas combustiveis

    Energy Technology Data Exchange (ETDEWEB)

    Bernardi Junior, Paulo

    2004-07-01

    In function of the necessity of electric energy as input of vital importance for the development of the country, this work aims at to offer plus an energy alternative for Brazil. The exploitation of the natural gas reserves recently discovered can be made through modern methods that give as priority the distributed generation and the low ambient impact. All these aspects can be gotten with the use of fuel cell, working with the remodelled natural gas. Increased to the factor of a low ambient impact in the proper generation, the project suggests that the generating source can be located next the consumer, diminishing still more the problems generated for transmission lines, fuel transport, etc. The fuel cell has received a great attention in the international community and some models, some already in commercial period of training, they have shown excellent possibilities of capsize to be one of the future technologies in the generation of electric energy with low ambient impact. (author)

  6. Polyester synthesis for application in PEMFC type fuel cells; Sintese de poliester para aplicacao em celulas a combustivel do tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Fiuza, R.P.; Souza, D.R. de; Fiuza, R.A.; Jose, N.M.; Boaventura, J.S. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica], e-mail: raigenis@gmail.com

    2006-07-01

    The PEMFC (Proton Exchange Membrane Fuel Cell), along the SOFC (Solid Oxide Fuel Cell), is the most important technology, among the various types of fuels cell. The PEMFC shows a large versatility of applications, both for stationary and mobile use. However the PEMFC presents high manufacture cost, directly impacting in the cost of the produced energy. This work contemplates the previews sulfonation of phtalic acid and its subsequent polymerization with glycerol, using as catalytic tin dibutyl-dilaurate. The obtained material has been characterized by DSC, TGA, FTIR, MEV, DRX and XRF. The gotten results indicated that phtalic acid was sulfonated and the increase of the sulfonation degree significantly increased the crystallinity of the sulfonated ftalico acid. Furthermore, the polymer produced from the sulfonated monomer presented adequate thermal resistance and a high content of conducting groups, necessary conditions for application as electrolyte in PEMFC. All these characteristics, particularly the low cost of the reagents and the ease of production process, make the sulfonated polyester membrane a promising candidate as fuel cell electrolyte. (author)

  7. Hydrogen purification for fuel cells using gold catalysts promoted with copper; Purificacao de hidrogenio para celulas a combustivel utilizando catalisadores de ouro promovidos com cobre

    Energy Technology Data Exchange (ETDEWEB)

    Mozer, Thiago Simonato; Passos, Fabio Barboza [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Engenharia Quimica e de Petroleo. Lab. de Reatores, Cinetica e Catalise (RECAT)]. E-mail: mozer@vm.uff.br

    2008-07-01

    The selective CO oxidation in the presence of H2 was investigated on Au catalysts promoted with different amounts of Cu. Au catalysts were prepared by the deposition-precipitation method and exhibited a satisfactory activity at 50 deg C with adequate selectivity. The addition of Cu to Au/Al{sub 2}O{sub 3} catalysts caused an increase in the selectivity to CO oxidation due to an interaction between Au and Cu on the surface of the catalysts. However, this beneficial effect was limited to an optimal content of Cu. Through the temperature programmed reduction (TPR) and ultraviolet visible diffuse reflectance spectroscopy (UV-vis DRS) techniques, the formation of small bimetallic Au-Cu particles was verified. The best result was obtained with 2.5% Au-0.5% Cu/Al{sub 2}O{sub 3} catalyst, the CO conversion was higher than 95% and the selectivity was around 70% at 50 deg C. The catalysts showed stable catalytic activity during 24 hours time -on-stream. In the presence of H{sub 2}O and CO{sub 2}, a small decrease in the CO conversion was observed in the stability test, probably due to the deactivation caused by the CO{sub 2} presence. (author)

  8. Multi-metallic anodes for solid oxide fuel cell applications; Anodos multi-metalicos para aplicacoes em celulas a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Restivo, T.A. Guisard; Mello-Castanho, S.R.H. [Instituto de Pesquisas Energeticas e Nucleares (CCTM/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Ciencia e Tecnologia dos Materiais; Leite, D. Will [Instituto de Pesquisas e Estudos Industriais (IPEI/FEI), Sao Bernardo do Campo, SP (Brazil). Fac. de Engenharia Industrial

    2009-07-01

    A new method for direct preparation of materials for solid oxide fuel cell anode - Ni- YSZ cermets - based on mechanical alloying (MA) of the original powders is developed, allowing to admix homogeneously any component. Additive metals are selected from thermodynamic criteria, leading to compacts consolidation through sintering by activated surface (SAS). The combined process MA-SSA can reduce the sintering temperature by 300 deg C, yielding porous anodes. Densification mechanisms are discussed from quasi-isothermal sintering kinetics results. Doping with Ag, W, Cu, Mo, Nb, Ta, in descending order, promotes the densification of pellets through liquid phase sintering and evaporation of metals and oxides, which allow reducing the sintering temperature. Powders and pellets characterization by electronic microscopy and X-ray diffraction completes the result analyses. (author)

  9. Fundamental aspects of the freezing of cells, with emphasis on mammalian ova and embryos. (Aspectos fundamentales de la congelacion de celulas, especialmente ovulos y embriones de mamiferos

    Energy Technology Data Exchange (ETDEWEB)

    Mazur, P.

    1980-01-01

    For most cells there exists an optimum cooling rate. Both supraoptimal rates and suboptimal rates can be very damaging. The optimal rate varies enormously from less than or equal to 1/sup 0/C/min for mammalian preimplantation embryos to greater than or equal to 800/sup 0/C/min for the human red cell. Death at supraoptimal rates is the result of the formation of intracellular ice and its recrystallization during warming. Intracellular ice occurs when cells are cooled too rapidly to allow them to equilibrate by the osmotic withdrawal of intracellular water. The definition of too rapid depends chiefly on the size of the cell and its permeability to water. Death at suboptimal rates is a consequence of the major alterations in aqueous solutions produced by ice formation. The chief effects are a major reduction in the fraction of the solution remaining unfrozen at a given temperature and a major increase in the solute concentration of that fraction. Presumably, slow freezing injury is a consequence of one or both of these solution effects. The introduction of molar concentrations of protective solutes (additives) greatly reduces both the fraction frozen and the concentration of electrolytes in the unfrozen channels and in the cell interior. Usually, freezing either kills cells outright or it yields survivors that retain full capacity to function. Although there is the possibility that in some cases survivors may in fact be impaired genetically, all evidence indicates that genetic damage does not occur. But there are clear examples in which freezing does induce nonlethal physiological damage. Particularly striking examples are found in certain mammalian sperm.

  10. DE LA MENTE A LA CELULA: IMPACTO DEL ESTRES EN PSICONEUROINMUNOENDOCRINOLOGIA/ FROM THE MIND TO THE CELL: THE IMPACT OF STRESS ON PSICONEUROIMMUNOENDOCRINOLOGY

    Directory of Open Access Journals (Sweden)

    Manolete S. Moscoso*

    2009-10-01

    Full Text Available Resumen:El presente artículo revisa el concepto del modelo psicobiosocial presentado por Engel y los primeros estudios sobre lanueva ciencia de la psiconeuroinmunoendocrinología, publicados por Solomon en la década del 60', y Ader en la década del70'. Se hace mención a los avances modernos en tecnología médica y de laboratorio como el punto de partida de un extensonúmero de estudios multidisclinarios durante la década del 80' que demuestran la relación entre el cerebro, y los sistemasnervioso, endocrino e inmune en el desarrollo de enfermedades. Se hace un análisis del concepto actual del estrés y unabreve revisión de las respuestas emocional y fisiológica descritas por Lazarus, Cannon, Selye, respectivamente. Una nuevavisión acerca del estrés es presentada y se hacen las distinciones correspondientes en relación a la respuesta comportamentaldel estrés, estilo de vida, y el estrés crónico. Finalmente se discute brevemente acerca del impacto negativo del estréscrónico sobre la salud del individuo.Abstarct:This article reviews the biopsicosocial model provided by Engel and the first studies in the field ofpsychoneuroimmunoendocrinology published by Solomon during the 60's and Ader during the 70's. it is pointed out thatadvances in medical technology and laboratory facilitated the beginning of studies in this field during the 80's. Extensivedata reports conclusive evidence of the interconnections between the brain, the nervous, endocrine and immune systems andthe development of disease. We discussed the emotional and physiological responses to stress as described by Lazarus,Cannon, and Selye. A new vision about stress is presented, taking into account the behavioral response to stress, life style,and chronic stress. Finally, we discussed briefly about the negative impact of chronic stress on health.

  11. CAMBIO FENOTIPICO DE LA CELULA DE LA MUSCULATURA LISA VASCULAR INDUCIDO POR PRODUCTOS DE GLICACION AVANZADA. PARTICIPACION DE LA NADPH OXIDASA.

    OpenAIRE

    SAN MARTIN ALMEYDA, MARIA ALEJANDRA

    2004-01-01

    Los productos de glicación avanzada (AGE) son un grupo heterogéneo de moléculas, resultado de la modificación covalente, irreversible y no enzimática de proteínas por glucosa. Los niveles plasmáticos y locales de AGE se encuentran aumentados en diabéticos 90p.

  12. KERAGAAN PEMIJAHAN BUATAN ANTARA IKAN PATIN SIAM (Pangasianodon hypophthalmus BETINA DAN IKAN PATIN JAMBAL (Pangasius djambal JANTAN DAN IKAN PATIN NASUTUS (Pangasius nasutus JANTAN

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2014-08-01

    Full Text Available Upaya pengembangan budidaya ikan patin jambal (Pangasius djambal dan ikan patin nasutus (P. nasutus sebagai komoditas ekspor ikan patin daging putih sulit direalisasikan, karena kematangan induk-induk betinanya sulit dicapai pada musim kemarau dan keterbatasan fekunditasnya, sehingga produksi massal benihnya terbatas. Salah satu upaya yang dapat dilakukan untuk meningkatkan produktivitas ikan patin daging putih adalah melalui program hibridisasi antara ikan patin siam (Pangasianodon hypophthalmus betina dengan ikan patin jambal jantan (menghasilkan ikan patin hibrida siam x jambal dan atau dengan ikan patin nasutus jantan (menghasilkan ikan patin hibrida siam x nasutus. Potensi budidaya kedua ikan patin hibrida tersebut berkaitan dengan produksi massal benihnya merupakan hal yang penting untuk diketahui. Penelitian ini bertujuan untuk mengevaluasi derajat fertilisasi, derajat penetasan, dan derajat deformitas larva kedua ikan patin hibrida tersebut. Hasil penelitian menunjukkan bahwa derajat fertilisasi ikan patin hibrida siam x nasutus sama (P>0,05 dengan ikan patin siam, ikan patin hibrida siam x jambal dan ikan patin nasutus, sedangkan ikan patin jambal memiliki derajat fertilisasi yang lebih rendah tetapi tidak berbeda (P>0,05 dengan ikan patin nasutus dan ikan patin hibrida siam x jambal. Derajat penetasan ikan patin hibrida siam x nasutus adalah sama (P>0,05 dengan ikan patin siam dan ikan patin hibrida siam x jambal, dan lebih tinggi (P0,05; sedangkan pada ikan patin jambal dan ikan patin nasutus lebih tinggi (P<0,05. Hasil-hasil tersebut menunjukkan bahwa produktivitas (produksi massal benih kedua ikan patin hibrida tersebut tinggi dan sama dengan ikan patin siam, sehingga potensial untuk dikembangkan sebagai ikan budidaya.

  13. EMBRIOGENESIS DAN PERKEMBANGAN LARVA PATIN HASIL HIBRIDISASI ANTARA BETINA IKAN PATIN SIAM ( Pangasianodon hypophthalmus Sauvage, 1878 DENGAN JANTAN IKAN PATIN JAMBAL ( Pangasius djambal Bleeker, 1846 DAN JANTAN PATIN NASUTUS ( Pangasius nasutus Bleeker, 1863

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2011-08-01

    Full Text Available Pengembangan budidaya ikan patin jambal maupun ikan patin nasutus untuk memenuhi permintaan pasar ekspor patin daging putih sulit direalisasikan karena keterbatasan fekunditas dan pematangan gonad induk betinanya. Salah satu upaya yang dapat dilakukan untuk meningkatkan produktivitas patin daging putih adalah melalui hibridisasi, yakni hibridisasi antara betina patin siam dengan jantan patin jambal maupun jantan patin nasutus. Hal ini dikarenakan patin siam memiliki keunggulan fekunditas yang tinggi, sedangkan patin jambal maupun patin nasutus memiliki keunggulan daging yang putih. Oleh karena itu, perlu dilakukan penelitian untuk mengetahui karakteristik patin hibrida tersebut, termasuk pada tahap-tahap awal kehidupannya. Penelitian ini bertujuan untuk mengetahui karakteristik embriogenesis dan perkembangan ontogeni morfologis larva patin hibrida tersebut. Hasil penelitian ini menunjukkan bahwa embriogenesis patin hibrida siam-jambal dan hibrida siam-nasutus serupa, tetapi perkembangan patin hibrida siam-nasutus sedikit lebih cepat. Pada suhu 28oC-29oC, larva patin hibrida siam-nasutus mulai menetas 20 jam setelah fertilisasi, berukuran panjang total 3,34±0,14 mm, dengan kantung kuning telur berukuran 0,71±0,28 mm3 yang terserap 50% pada umur 24 jam dan relatif habis terserap pada umur 54 jam. Larva patin hibrida siam-jambal mulai menetas 21 jam setelah fertilisasi, berukuran panjang total 3,47±0,13 mm, dengan kantung kuning telur berukuran 0,42±0,08 mm3 yang terserap 50% pada umur 30 jam dan habis terserap pada umur 60 jam. Perkembangan larva kedua patin hibrida tersebut hingga menyerupai morfologi ikan patin dewasa juga relatif serupa, tetapi patin hibrida siam-nasutus menunjukkan keragaan pertumbuhan yang lebih bagus, menghasilkan heterosis berdasarkan pertambahan panjang total selama 10 hari masa pemeliharaan sebesar 20,20%, sedangkan pada patin hibrida siam-jambal sebesar -4,15%.

  14. Evaluation of the radio modifier effect of propolis on chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with 60-CO; Avaliacao do efeito radiomodificador da propolis em celulas de ovario de hamster chines (CHO-K1) e em celulas tumorais de prostata (PC3), irradiadas com CO-60

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Geyza Spigoti

    2011-07-01

    In the last decades, it has been given a great interest to investigations concerning natural, effective, nontoxic compounds with radioprotective potential together with the increasing utilization of different types of ionizing radiation for various applications. Among them propolis, a resinous compound produced by honeybees (Apis mellifera), has been considered quite promising, since it presents several advantageous biological characteristics, i. e., anti-inflammatory, antimicrobial, anticarcinogenic, antioxidant and also free radical scavenging action. The purpose of the present study was to evaluate the effect of Brazilian propolis, collected in the State of Rio Grande do Sul, on Chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with {sup 60}Co {gamma} radiation. For this purpose, three interlinked parameters were analyzed: micronucleus induction, cell viability and clonogenic death. The choice of these parameters was justified by their biological significance, in addition to the fact that they are readily observable and measurable in irradiated cells. The cytogenetic data obtained showed a radioprotective effect of propolis (5-100 {mu}g/ml) in the induction of DNA damage for both cell lines, irradiated with doses of 1 - 4 Gy. The cytotoxicity assay, however, showed a prominent antiproliferative effect of propolis (50 - 400{mu}/ml) in PC3 cells irradiated with 5 G{gamma}. The survival curves obtained were adequately fitted by a linear-quadratic model, where the {alpha} coefficient was higher in CHO-K1 cells. Concerning the clonogenic capacity, PC3 cells were more radiosensitive than CHO-K1 cells at the higher doses of the survival curve. Propolis at the concentrations of 30 - 100 {mu}g/ml, did not influence the clonogenic potential of PC3 cells, since the survival curves, associated or not with propolis, were found similar, although the combined treatment in CHO-K1 cells exhibited a stimulating proliferative effect. The data obtained in vitro showed a potential use of propolis AF-08, a natural and nontoxic compound, in the prevention against the adverse effect of ionizing radiation, at the doses and concentrations here analyzed. (author)

  15. Effects on the work of a direct ethanol fuel cell as function of compacting force applied to the cell; Efeitos no funcionamento de uma celula a combustivel com alimentacao direta de etanol (CCADE) em funcao da forca de compactacao aplicada a celula

    Energy Technology Data Exchange (ETDEWEB)

    Belchior, P.M.; Forte, M.M.C. [UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Serafin, R.; Fusiger, V.; Carpenter, D. [FURB - Fundacao Universidade Regional de Blumenau, SC (Brazil)

    2010-07-01

    This experiment aimed to verify the influence due to the variation of the compacting force applied to a DEFC. According to the tests, as the increase of cell compacting, the performance improves significantly. According applied tests, when the high electrical conductivity components are used, small differences of the cell compacting can generate a large difference on the total ohmic resistance. Through the tests one can conclude that with the increase of cell compacting, the contact probably increase among the distribution of reagents and the diffuser layer, improving the cell performance. (author)

  16. [Therapeutic potential of bone marrow stem cells in cerebral infarction].

    Science.gov (United States)

    Sánchez-Cruz, Gilberto; Milián-Rodríguez, Lismary

    2015-05-16

    Introduccion. Las celulas madre constituyen una alternativa terapeutica que se encuentra en fase de experimentacion para el infarto cerebral. Objetivo. Mostrar la evidencia cientifica existente sobre el potencial terapeutico de las celulas madre de la medula osea en esta enfermedad. Desarrollo. El infarto cerebral representa el 80% de las enfermedades cerebrovasculares. La trombolisis constituye la unica terapia aprobada, pero, por su estrecha ventana terapeutica, solo se aplica a un bajo porcentaje de los pacientes. De manera alternativa, los tratamientos neurorrestauradores, como el de celulas madre, pueden aplicarse en periodos mas prolongados. Por esta razon se efectuo una busqueda bibliografica en PubMed con el empleo de las palabras clave 'stem cells', 'bone marrow derived mononuclear cells' y 'stroke'. Se encontraron evidencias de seguridad y eficacia de dichas celulas en diferentes momentos evolutivos del infarto cerebral. Se identificaron estudios que en clinica y preclinica las recolectaron por puncion medular y en sangre periferica, y las trasplantaron directamente en el area infartada o por via intravascular. El efecto terapeutico se relaciona con sus propiedades de plasticidad celular y liberacion de factores troficos. Conclusiones. El concentrado de celulas mononucleares autologas, obtenido en sangre periferica o por puncion de la medula osea, y trasplantado por via intravenosa, es una factible opcion metodologica que permitira rapidamente incrementar el numero de ensayos clinicos en diferentes etapas evolutivas del infarto cerebral. Esta terapia muestra seguridad y eficacia; sin embargo, deben ampliarse las evidencias que avalen su generalizacion en humanos.

  17. [Erythropoietin as a protective factor in rat CNS cells receiving radiotherapy -an in vitro study].

    Science.gov (United States)

    Gomez-De la Riva, Álvaro; Isla-Guerrero, Alberto; García-Grande, Antonio

    2014-03-01

    Objetivo. Investigar el efecto de la eritropoyetina en cultivos celulares de corteza cerebral de ratas cuando se administra radioterapia. Materiales y metodos. El estudio se desarrolla con la obtencion de corteza cerebral de embriones de 17 dias de preñez de ratas Wistar. Las celulas cultivadas despues de 72 horas de la extraccion de la corteza se dividieron en dos grupos, a uno de ellos se le administro eritropoyetina alfa a una concentracion de 30 pM y el otro era el grupo control. A los dos grupos de celulas se les radio con 6 Gy mediante un aparato Phoenix. Tras la radioterapia permanecieron 24 horas en la incubadora antes de fijarlas. Las celulas fueron fijadas con formaldehido al 4%. A continuacion, con la tecnica de TUNEL, se valoro el numero de celulas apoptoticas en los cultivos radiados. Resultados. Se observo un porcentaje de apoptosis del 25,22% del grupo de cultivo sin eritropoyetina, mientras que en el grupo de celulas radiadas con eritropoyetina fue del 15,5%. Las variables cuantitativas se analizaron mediante el test t de Student y el resultado de la comparacion entre los dos grupos fue estadisticamente significativo (p sistema nervioso central de ratas por radiacion. Esto abre nuevos campos para la investigacion del efecto protector del sistema nervioso.

  18. Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Poncã' mandarin Desenvolvimento de embriões globulares de citros provenientes de hibridação entre laranjeira 'Pêra Rio' x tangerineira 'Poncã'

    Directory of Open Access Journals (Sweden)

    Edvan Alves Chagas

    2003-12-01

    Full Text Available This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3 on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1 MT concentrations (0%, 50%, 100%, 150% and 200% supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2 vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200% supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3 activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1 supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1. After the inoculation, the embryos were kept in a growth room for 90 days at 27 ± 1ºC, in a 16-hour photoperiod with 32 µmol.m-2.s-1 of irradiance. The best development of embryos at the globular stage was achieved using 50% and 100% of the MT medium plus 60 g.L-1 and 90 g.L-1 of sucrose, respectively, supplemented with 0.01 mg.L-1 of GA3. The addition of activated charcoal or vitamins in the MT medium has shown to be unnecessary to the development of globular embryos.Objetivou-se estudar a influência de diversas concentrações do meio MT, sacarose, vitaminas, carvão ativado e ácido giberélico no cultivo de embriões imaturos oriundos do cruzamento entre laranjeira 'Pêra Rio' x tangerineira 'PONCÃ' . Os embriões foram excisados sob condições assépticas e inoculados em 15 mL do meio de cultura MT, de acordo com cada experimento a seguir: 1 concentrações do meio de cultura MT (0%, 50%, 100%, 150% e 200% combinados com 0, 30, 60 e 90 g.L-1 de sacarose; 2 concentrações de vitaminas do meio MT (0%, 50%, 100%, 150% e 200% combinados com 0, 30, 60 e 90 g.L-1 de sacarose e; 3 concentrações de carvão ativado (0; 0.5; 1; 1.5 e 2 g.L-1 combinados com GA3 (0; 0.01; 0.1; 1 e 10 mg.L-1. Após a inoculação, os embriões foram mantidos por 90 dias em sala de crescimento à temperatura de 27±1ºC, fotoperíodo de 16 horas e irradiância de 32 µmol.m-2.s-1. A utilização de 50% e 100% do meio MT associado a 60 e 90 g.L-1 de sacarose, respectivamente, acrescido de 0.01 mg.L-1 de GA3, proporcionou melhor desenvolvimento de embriões globulares. Não houve necessidade da adição de carvão ativado e vitaminas no meio MT para o cultivo de embriões globulares.

  19. Evolution of the energy content and emissions of CO{sub 2} associated with Brazilian economic production between 1990 and 2030: a hybrid approach; Evolucao do conteudo energetico e das emissoes de CO{sub 2} associadas a producao economica brasileira entre 1990 e 2030: uma abordagem hibrida

    Energy Technology Data Exchange (ETDEWEB)

    Pereira Junior, Amaro Olimpio; Soares, Jeferson Borghetti; Oliveira, Ricardo Gorini de; Queiroz, Renato Pinto de [Empresa de Pesquisa Energetica (EPE), Rio de Janeiro, RJ (Brazil)], e-mail: amaro.pereira@epe.gov.br, e-mail: jeferson.soares@epe.gov.br, e-mail: ricardo.gorini@epe.gov.br, e-mail: renato.queiroz@epe.gov.br

    2008-07-01

    This study proposes um hybrid approach to analyze the results of the Energy National Plan 2030, performed by EPE, a state owned Enterprise of Energy Research. The model integrates the energy data to macroeconomics, giving them consistence, from the input-output analysis. The resultant matrixes from this methodology are use to analyze the evolution of energy content and the CO{sub 2} emissions associated to the economic production of choose sectors. (author)

  20. Optimization of the production process of hybrid and multivalent formulation Bombesin/RGD for the opportune detection of breast cancer; Optimizacion del proceso de fabricacion de la formulacion hibrida y multivalente Bombesina/RGD para la deteccion oportuna de cancer de mama

    Energy Technology Data Exchange (ETDEWEB)

    Robles M, M.

    2013-07-01

    The radiopharmaceuticals of third generation are used in nuclear medicine to obtain images of specific molecular targets, and they are unique in their capacity to detect in vivo specific biochemical sites as receptors that are over-expressed in diverse illness. In cancer cells several types of receptors are over-expressed, as the integrin s α(v)β(3) and α(v)β(5) that specifically recognize the sequence RGD (Arginine-Glycin-Ac. Aspartic) and gastrin-releasing peptide that recognizes specifically to the peptide Lys{sup 3}-Bombesin. The integrin s α(v)β(3) and α(v)β(5) are involved in the tumor angio genesis processes and the gastrin-releasing peptide is over-expressed in breast and prostate cancer. The molecular recognition of the specific receptors is the basis to be utilized as targets of the radiopharmaceuticals {sup 99m}Tc-HYNIC-Bombesin and {sup 99m}Tc-HYNIC-RGD. In this work was developed a lyophilized pharmaceutical formulation effective, stable and safe for the simultaneous obtaining of the radiopharmaceuticals {sup 99m}Tc-HYNIC-Bombesin ({sup 99m}Tc-EDDA/HYNIC-Lys{sup 3}-Bombesin) and {sup 99m}Tc-HYNIC-RGD ({sup 99m}Tc EDDA/HYNIC-E-[c(RGDfK)]{sub 2}). Later on the production process of the product HYNIC-Bombesin/RGD-Sn was optimized using a factorial design and the formulation was transferred to the production plant of radiopharmaceuticals of the Instituto Nacional de Investigaciones Nucleares (ININ). The optimized formulation is described in the following chart: HYNIC-[Lys{sup 3}]-Bombesin - 12.5 μg; HYNIC-E-c[RGDfK]{sub 2} - 12.5 μg; Stannous chloride (SnCl{sub 2}) - 20 μg; Ethylenediamine diacetic acid (EDDA) - 10 mg; N-tris(hydroxymethyl)methyl glycin (Tricine) - 20 mg; Mannitol - 50 mg. The production process was validated and were carried out the stability studies under refrigeration conditions. (Author)

  1. Biokinetics and dosimetry of a hybrid formulation of {sup 9{sup m}}Tc-BN and {sup 99m}Tc-RGD{sub 2} starting from optic images in a murine model; Biocinetica y dosimetria de una formulacion hibrida de {sup 99m}Tc-BN y {sup 99m}Tc-RGD{sub 2} a partir de imagenes opticas en un modelo murino

    Energy Technology Data Exchange (ETDEWEB)

    Cornejo A, L. G.

    2015-07-01

    This work has the purpose of evaluate the biokinetics and absorbed dose of radiation of hybrid formulation {sup 99m}Tc-BN /{sup 99m}Tc-RGD{sub 2} in a murine model by optical imaging techniques using the multimodal preclinical in vivo image system Xtreme. The used method were the {sup 99m}Tc-BN, {sup 99m}Tc-RGD{sub 2} and {sup 99m}Tc-BN/{sup 99m}Tc-RGD{sub 2} formulas, with specific recognition for GRPr and the integrin s α(v)β(3) and α(v)β(5) respectively, was injected in the vein tail of three nude mousses with induce breast cancer tumors (cell line T-47-D), by the preclinical multimodal imaging system Xtreme (Bruker), optical images in different times was acquired (5, 10, 20 min, 2 and 24 h), using Images Processing Toolbox of MATLAB these images was transform from RGB format to gray scales and sectioned in five independent images corresponding to heart, kidneys, bladder and tumor areas. The intensity of each images was computed in counts per pixel, then those intensities was corrected for background, attenuation and scattering, using different factors for each phenomena previously calculated. Finally the activity values quantified vs time was fitted into a biokinetic model to obtain the disintegrations number and cumulate activities in each organ. With these data the radiation absorbed dose were calculated using MIRD methodology. Results: The number of disintegration and absorbed dose calculated in MBq h/MBq and mGy/MBq, of injected mouse with the {sup 99m}Tc-BN/{sup 99m}Tc-RGD{sub 2} formulation, was: 0.035 ± 0.65 E-02, 0.25 x 10{sub -5} ± 0.46 E-07; 0.393 ± 0.51 E-1, 2.85 E-05 ± 3.7 E-06; 0.306 ± 0.21 E-01, 2.11 E-05 ± 1.45 E-06 and 0.151 ± 0.19 E-01, 1.09 E-05 ± 1.42 E-06 , in heart, kidneys, bladder and tumor, respectively. The number of disintegration obtained in kidneys is comparable to those reported for Trinidad B. 2014 Conclusions: Our results demonstrated that using optical images and a code for image analyses development in MATLAB, could achieve comparable quantitative results as the conventional technic s. (Author)

  2. Study of cross-linking reactions induced by gamma rays in hybrid membranes of Bisphenol-A-Polysulfone and precipitated silica; Estudo da formacao de ligacoes cruzadas por irradiacao gama em membranas hibridas de Polissulfona Bisfenol-A e silica precipitada

    Energy Technology Data Exchange (ETDEWEB)

    Furtado Filho, Acacio Antonio M., E-mail: facacio@ctex.eb.br [Laboratorio de Quimica Militar, CTEx, Rio de Janeiro, RJ (Brazil); Gomes, Ailton de S.; Lopes, Lea; Benzi, Marcia R. [Instituto de Macromoleculas Professora Eloisa Mano, UFRJ, Rio de Janeiro, RJ (Brazil)

    2011-07-01

    In this work the bisphenol-A-polysulfone (PSF) was sulfonated using trimethyl silyl chlorosulfonate [(CH{sub 3}){sub 3}SiSO{sub 3}Cl] as a mild sulfonating agent in a homogeneous solution of dichloroethane. The sulfonation reaction was confirmed by acid-base titration and FTIR-spectroscopy analysis. The hybrid membranes were obtained by casting the sulfonated bisphenol-A-polysulfone (SPSF) and precipitated silica Tixosil{sup R} 333 solutions in N-N-dimethylacetamide. Cross-linking in the hybrid membranes was obtained by irradiation, with doses ranging from 5 to 30 kGy using gamma ray from a {sup 60}Co source. The water uptake and the swelling of the membranes were estimated by measuring the change in weight between dry and wet conditions. The conductivity of the membranes in acid form was measured with the ac impedance technique using a PGSTAT30 frequency response analyzer. The hybrid cross-linked membranes have conductivity close to 10-1 S.cm{sup -1} at 100% RH and 80 deg C. Electrochemical performances, thermo-mechanical stability and low cost make this cross-linked SPSF hybrid membrane an attractive material for fuel cells using a proton exchange membrane. (author)

  3. Tratamento da Osteonecrose da Cabeça Femoral com celulas progenitoras autólogas em anemia falciforme Femoral Head Necrosis treatment with autologous stem cells in sickle cell disease

    Directory of Open Access Journals (Sweden)

    Gildásio Cerqueira Daltro

    2008-01-01

    Full Text Available OBJETIVO: Avaliação da segurança e eficácia do uso de células progenitoras autólogas da medula óssea (CMMO no tratamento da Osteonecrose da Cabeça Femoral (OCF de pacientes portadores de anemia falciforme. MÉTODOS: Foram estudados 8 pacientes portadores de anemia falciforme, com OCF nos estágios I e II (classificação de Ficat e Arlet. As CMMO retiradas da crista ilíaca posterior foram concentradas e reinfundidas na área central da osteonecrose. Os principais parâmetros avaliados foram segurança, sintomas clínicos e progressão da doença, através da avaliação clínica (Harris Hip Score e radiológica. RESULTADOS: A maior parte dos pacientes (7 em 8 referiu melhora dos sintomas após o tratamento. Não houve complicações durante o procedimento anestésico e cirúrgico. A medida do escore (Harris Hip Score no pré-operatório foi 78,5 +/- 6,2 pontos, com aumento significativo destes valores no pós-operatório (98,3 +/- 2,5 pontos (pPURPOSE: To assess the efficacy and safety of autologous bone-marrow mononuclear cells (BMMC implantation in necrotic lesions of the femoral head in patients with sickle cell disease. METHODS: We studied eight patients with stage-I or -II femoral head osteonecrosis according to the system by Ficat and Arlet. BMMCs were harvested and re-infused into the necrotic zone. The primary endpoints studied were safety, clinical symptoms and disease progression, these being assessed according to the Harris hip score (HHS and to X-ray studies. RESULTS: After eight months, seven of the eight patients reported improvement from symptoms. There were no complications during anesthetic and surgery procedures. There was a significant postoperative increase in the HHS (98.3 +/- 2.5 points compared to preoperative HHS (78.5 +/- 6.2 points (p< 0.001. X-ray evaluation and cell parameters were found to be favorable. CONCLUSION: The autologous bone-marrow mononuclear cells implantation seems to be a safe and effective treatment for early stages of femoral head osteonecrosis in patients with sickle cell disease. Although promising, the interpretation of these early results is limited due to the small sample and to the short duration of follow-up. Further studies and advanced cellular assays are required to confirm the results.

  4. Obtainment of TiO{sub 2} powders solar cells photo electrodes dye sensitized; Obtencao de pos de TiO{sub 2} para fotoeletrodos de celulas solares sensibilizados por corante

    Energy Technology Data Exchange (ETDEWEB)

    Forbeck, Guilherme; Folgueras, Marilena V., E-mail: guilhermeforbeck@hotmail.com [Universidade do Estado de Santa Catarina (PGCE/UDESC), SC (Brazil). Programa de Pos Graduacao em Ciencia e Engenharia de Materiais; Chinelatto, Adilson L. [Universidade Estadual de Ponta Grossa (UEPG), PR (Brazil). Programa de Mestrado em Engenharia e Ciencia dos Materiais

    2012-07-01

    Titanium dioxide in its polymorphic anatase phase, presents interesting properties for solar cells photo electrodes dye sensitized such as the forbidden energy band, high refractive index and high constant dielectric. In this study, powders of nanometric titanium dioxide were produced with predominantly the anatase phase and high surface area. We used the sol-gel method, and titanium tetraisopropoxide as a precursor, which was hydrolyzed in nitric acid solution. The obtained powder was heated to 450 ° C, varying the time for each lot (0, 20 or 120 minutes). The powders were characterized by X-ray diffraction, atomic force microscopy and surface area analysis. For all lots nanosized crystallites predominated. It was observed that in the batch with 120min heating an increase rutile content. The TiO{sub 2} with 20min heating showed high surface area, greater than that of TiO{sub 2} as taken reference.

  5. In vitro study of the effects of radio frequency generated for plasma in neoplastic cells HT-29; Estudo in vitro dos efeitos da radiofrequencia gerada por plasmas em celulas neoplasicas HT-29

    Energy Technology Data Exchange (ETDEWEB)

    Andrighetto, Daniela; Dornelles, Eduardo Bortoluzzi; Cruz, Ivana Beatrice Manica da; Lüdke, Everton, E-mail: daniela.andrighetto@hotmail.com, E-mail: dornellesedu@gmail.com, E-mail: ibmcruz@hotmail.com, E-mail: evertonludke@gmail.com [Universidade Federal de Santa Maria (UFSM), RS (BRazil)

    2014-07-01

    The goal of this study is to develop an in vitro irradiation cell system with controllable irradiation intensities of 27 MHz produced by an argon plasma column with variable amplitude modulation in the 100-700 kHz range. This paper presents and discusses a proposed experiment, with toxicity analysis (DNA Picogreen®) and cell viability (MTT assay) in the radiation-induced HT-29 cell line (colon adenocarcinoma). The data allow us to observe that cellular toxicity effects may occur with exposure to fields produced by argon plasma with intensities on the order of at least 3.2 W / cm2 and exposure times above 3.5 hours continuously. An analysis of cell populations for cell toxicity tests using the Student's t-test did not show significant changes (p <0.05) in the amount of DNA released by the action of radiofrequency, although it has been found that cell viability (MTT) is not significantly altered by long exposures to radiation induced plasma RF signals in 27 MHz (p> 0.34). Cytotoxic effects due to the destruction of cell wall by heating the samples were not detected in any of the tests.

  6. Co-sintering of CGO/NIO-CGO bilayers for solid oxide fuel cell; Co-sinterizacao de bi-camadas anodo/eletrolito para celulas a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Neto, P.P.B.; Grilo, J.P.F.; Souza, G.L.; Macedo, D.A.; Paskocimas, C.A.; Nascimento, R.M., E-mail: pbritoneto@gmail.com [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2012-07-01

    Reducing the operating temperature of solid oxide fuel cells (SOFC) for the range between 500 and 700°C is one of the challenges which more has aroused the interest of research in SOFC in recent years. In this context, the bilayer anode/electrolyte composed of a porous support based on Ni-doped ceria (anode) and a ceria doped gadolinia (CGO) electrolyte, presents itself as one of the half-cell configurations of the most interest towards the production of electricity in the operating logic of a SOFC. In this work, CGO films were successfully prepared on NiO-CGO substrates using the resources of the screen-printing technique. The bi-layers were co-sintered between 1350 and 1450 ° C for 4 h and then characterized by scanning electron microscopy and energy dispersive X-ray spectroscopy (EDS). The results showed good adhesion at the film/substrate interface and no cracks in the films. (author)

  7. The proliferation induced by hyperthermia in NB69 cells is offset by a radar-like signal; La proliferacion inducida por hipertermia en celulas NB69 es contrarrestada por una senal de tipo radar

    Energy Technology Data Exchange (ETDEWEB)

    Trillo Ruiz, M. A.; Martinez Pascual, M. A.; Cid Torres, M. A.; Pague de la Vega, J. E.; Chacon Vargas, L.; Ubeda Maeso, A.

    2011-07-01

    The present study describes the proliferative response of the cell line NB69 human neuroblastoma, the simultaneous exposure to two physical agents: mild hyperthermia (+1 degree centigrade) and a pulsed signal RFsubthermal.

  8. Treatment of transparent conductive oxides by laser processes for the development of Silicon photovoltaic cells; Tratamiento de oxidos conductores transparentes por procesos laser para el desarrollo de celulas fotovoltaicas de silicio

    Energy Technology Data Exchange (ETDEWEB)

    Canteli Perez-Caballero, D.

    2015-07-01

    Transparent conductive oxides (TCOs) are heavily doped oxides with high transparency in the visible range of the spectrum and a very low sheet resistance, making them very attractive for applications in optoelectronic devices. TCOs are widely found in many different areas such as low emissivity windows, electric contacts in computers, televisions or portable devices, and, specially, in the photovoltaic (PV) industry. PV industry is mainly based on mono- and multicrystalline silicon, where TCOs are used as anti-reflective coatings, but the search for cheaper, alternative technologies has led to the development of thin film PV technologies, where TCOs are used as transparent contacts. With the maturation of the thin film PV industry, laser sources have become an essential tool, allowing the improvement of some industrial processes and the development of new ones. Because of the interest on a deeper understanding of the interaction processes between laser light and TCOs, the laser ablation of three of the most important TCOs has been studied in depth in the present work. (Author)

  9. Effect of the combined action of gamma radiation and static fields in human cells;Efeito da acao combinada de radiacao gama e campo eletrico estatico em celulas humanas

    Energy Technology Data Exchange (ETDEWEB)

    Moron, Michelle Mendes

    2008-07-01

    Our goal is the study in human cells of the effect resulting from the association of irradiation with exposure to exogenous static electric fields. The T47D cell line of breast cancer cells was irradiated with gammas in the 0 8 Gy doses range. The viability of this T47D cells exposed to both gamma radiation and 1.250 V/cm static electric field (SEF) was about 12% lower than when only irradiated. The sole exposure of the cells to SEF by 24 and 72 hours did not induce toxicity. Immunofluorescence runs carried out in irradiated normal MRC5 cell line of human lung fibroblast have quantified the expression of the g-H2AX histone. The amount of phosphorylated histones was approximately 40% higher after irradiation with 2 Gy plus exposure to a SEF by 1 hour, showing that the electric field negatively interfered in the repairing process of the DNA double strand breaks. The flow cytometry analysis with FACS showed that in T47D cells treated with 1 and 2 Gy by 24 hours the SEF also negatively interfered in the DNA repairing process, as evidenced by the higher accumulation of cells in the S phase. (author)

  10. Polymers and composites synthesis and characterization for application on PEM type fuel cells; Sintese e caracterizacao de polimeros e compositos para aplicacao em celulas a combustivel do tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Fiuza, Raigenis da Paz; Souza, Daniele Ribeiro; Barreto, Ednardo Gomes; Boaventura Filho, Jaime Soares; Jose, Nadia Mamede [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil)]. E-mail: raigenis@gmail.com

    2006-07-01

    The PEM (proton exchanging membrane) type fuel cell presents good potential for the energy production without the residue generation. However, its manufacture presents high costs for commercial application, mainly due to the electrolyte. Sulfonated Peek (polish-ether-ether-ketone) supported or auto immobilized the in a silicone matrix is an interesting alternative as electrolyte for PEM fuel cells. The commercial PEEK in powder form was functionalized with sulfuric acid, giving the SPEEK (Sulfonated PEEK). The membranes were produced by hot pressing the SPEEK immobilized in a silicone matrix produced by the sol-gel process. The membranes obtained were characterized by DRX, FTIR, TGA, MEV, DSC and protonic conductivity measurements. (author)

  11. Synthesis and ceramic processing of zirconia alumina composites for application as solid oxide fuel cell electrolytes; Sintese e processamento de compositos de zirconia-alumina para aplicacao como eletrolito em celulas a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Rafael Henrique Lazzari

    2007-07-01

    The global warmness and the necessity to obtain clean energy from alternative methods than petroleum raises the importance of developing cleaner and more efficient systems of energy generation, among then, the solid oxide fuel cell (SOFC). Cubic stabilized zirconia (CSZ) has been the most studied material as electrolyte in SOFC, due to its ionic conductivity and great stability at operation conditions. However, its low fracture toughness difficulties its application as a thin layer, what could lead to an improvement of cell efficiency. In this sense, the alumina addition in CSZ forms a composite, which can shift its mechanical properties, without compromising its electrical properties. In this work, coprecipitation synthesis route and ceramic processing of zirconia-alumina composites were studied, in order to establish optimum conditions to attain high density, homogeneous microstructure, and better mechanical properties than CSZ, without compromising ionic conductivity. For this purpose, composites containing up to 40 wt % of alumina, in a 9 mol % yttria-stabilized zirconia (9Y-CSZ) matrix were evaluated. In order to optimize the synthesis of the composites, a preliminary study of powder obtaining and processing were carried out, at compositions containing 20 wt % of alumina, in 9Y-CSZ. The ceramic powders were characterized by helium picnometry, X-ray diffraction, scanning electronic microscopy, transmission electronic microscopy, thermogravimetry, differential scanning calorimetry, granulometry by laser diffraction and gas adsorption (BET). The characterization of sinterized compacts were performed by X-ray diffraction, scanning electron microscopy, optical microscopy, density measurements, Vickers indentation and impedance spectroscopy. The obtained results show that the alumina addition, in the 9Y-CSZ matrix powders, raises the specific surface area, promotes deagglomeration of powders and elevates the oxides crystallization temperature, requiring higher energetic thermal treatments to attain high densities. In relation to the sintered products, it was confirmed the excellent homogeneity and crystallinity of microstructure provided by the chosen route, the restriction of grain growth by alumina addition, raise of hardness and fracture toughness, and higher ionic conductivity, even tough a lower bulk conductivity. These results indicate that the addition of 5 wt % alumina in CSZ matrix allows the application of this material as solid oxide fuel cell electrolytes, due to its better fracture toughness and ionic conductivity, compared to yttria-stabilized cubic zirconia ceramics. (author)

  12. Structural and optical properties of electrodeposited culnSe{sub 2} thin films for photovoltaic solar cells; Propiedades estructurales y opticas de laminas delgadas de CulnSe2 electrodepositadas para su aplicacion en celulas solares fotovoltaicas

    Energy Technology Data Exchange (ETDEWEB)

    Guillen, C.; Herrero, J.; Galiano, F.

    1990-07-01

    Optical an structural properties of electrodeposited copper indium diselenide, CulnSe2, thin films were studied for its application in photovoltaic devices. X-ray diffraction patterns showed that thin films were grown in chalcopyrite phase after suitable treatments. Values of Eg for the CulnSe2 thin films showed a dependence on the deposition potential as determined by optical measurements. (Author) 47 refs.

  13. Union of {sup 99m} Tc-HYNIC-TOC at the somatostatin receptors in cells of pancreas cancer; Union del {sup 99m} Tc-HYNIC-TOC a los receptores de somatostatina en celulas de cancer de pancreas

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez C, J. [Facultad de Medicina, UAEM, 50000 Toluca, Estado de Mexico (Mexico); Ramirez I, M.T. [INCMNSZ, Vasco de Quiroga Num. 15, Tlalpan, 14000 Mexico D.F. (Mexico); Ferro F, G.; Pedraza L, M. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2005-07-01

    The radiation toxic effects have been used in therapy however much 50 years. The absorbed radiation dose can be determined at cellular level using cancerous cell cultures. If the deposited In vitro radiation dose coming from similar activities of several therapeutic radiopharmaceuticals it can compare it will be possible to choose the therapeutic radiopharmaceutical that it offers better dosimetric characteristics for the patient. The objective of this original investigation was to determine the union percentage of the octreotide {sup 99m}Tc-HYNlC-TOC to the somatostatin receivers in cells of cancer pancreas as well as the internalization, externalization and cellular viability. It was used the octapeptide, (octreotide, TOC) labelled with {sup 99m}Tc by means of the HYNIC chelating agent (6-hydrazine pyridine-3-carboxylic acid) and 3 cellular lines of murine pancreas cancer (AR42J), of cancer of human pancreas (CAPAN) and of one negative cellular line for somatostatin receivers (WRL-68). The {sup 99m}Tc-HYNIC-TOC was compared against two negative proofs for somatostatin receivers: the peptide {sup 99m}Tc-UBI and the {sup 99m}TcO{sub 4}. The cellular lines were conserved in the synthetic media Dulbecco-Eagle. After 2, 4 and 24 h of exhibition to the radiation, the cells are picked up and its are determined the viability by count in a Neubauer camera using tripan blue. In the same times it was calculated the union percentage of the radiopharmaceutical to the cells and the internalization (union to the cytoplasm) and the externalization (union to membrane receivers). With those figures it was calculated the absorbed radiation dose at cellular level. Results: At 4 hours the union percentage of the {sup 99m}Tc-HYNlC-TOC to the AR42-J cells was 6.83 times greater than for the WRL-68 control cells of human papilloma, (without receivers of the somatostatin) and for the CAPAN them 4 times greater than for the same cells used as negative control, for the case of the {sup 99m}Tc-UBI and the {sup 99m}TcO{sub 4} one doesn't observe a significant difference and the percentage of viability of the AR42-J cells due by the {sup 99m}Tc-HYNlC-TOC it was 20% smaller in comparison with {sup 99m}Tc-UBI and {sup 99m}TcO{sub 4}. The internalization percentage of the AR42-J cells was of 43.28 {+-}1.62 due to the somatostatin receivers that its enter together with the radiopharmaceutical at the interior of the cell and 56.72 {+-}3.25 are together to the cellular membrane. (Author)

  14. Synthesis of precursor the base of indene for polyelectrolyte an membrane production for application in fuel cell; Sintese de precursores a base de indeno para producao de polieletrolitos e membranas para uso em celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Brum, F.J.B.; Laux, F.N.; Haack, M.S.; Forte, M.M.C. [Universidade Federal do Rio Grande do Sul. Escola de Engenharia, Porto Alegre, RS (Brazil)

    2010-07-01

    Monomers with vinyl bond can be polymerized via a cationic mechanism using acid catalysts. This study aimed to obtain homo and copolymers of styrene and indene via cationic mechanism and the functionalization of sulfonic groups to the production of membranes for fuel cells. Polymers and poly electrolytes were characterized by infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and gel permeation chromatography (GPC/SEC). The degree of sulfonation of the polymers was determined by titration and evaluated for these films to the degree of swelling in water, ion exchange capacity and analysis of electrochemical impedance spectroscopy. Membranes prepared with polyindene and PVA were tested in an apparatus of the fuel cell. (author)

  15. Performance of direct ethanol fuel cells as function of using of compressed air; Desempenho de celulas a combustivel com alimentacao direta de etanol em funcao do uso de ar comprimido

    Energy Technology Data Exchange (ETDEWEB)

    Belchor, P.M. [UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Berns, B.A.; Ferreira, R.C.; Goldbach, A.; Carpenter, D. [FURB - Fundacao Universidade Regional de Blumenau, Blumenau, SC (Brazil)

    2010-07-01

    This paper compared the performance of a direct ethanol fuel cell (CCADE) cathode feeding with air replacing the pure oxygen. The results have shown that the small decreasing of the yield of the cell under both practical and experimental situations, by the use of air replacing pure oxygen, it completely acceptable as function of great diminishing of operational costs. (author)

  16. Preparation of gas diffusion layers for PEMFC fuel cells using carbon fibers; Elaboracao de uma camada de difusao de gas a partir de fibras de carbono para aplicacao em celulas combustiveis do tipo PEMFC

    Energy Technology Data Exchange (ETDEWEB)

    Santos, J.N.; Kunsti, S.R.; Malfatti, C.F. [Universidade Federal do Rio Grande do Sul - Departamento de Metalurgia (PPGEM) - Laboratorio de Pesquisa em Corrosao (LAPEC), Porto Alegre, RS (Brazil); Vargas, J.V.C. [Universidade Federal do Parana - Departamento de Engenharia Mecanica, PR (Brazil); Amico, S.C. [Universidade Federal do Rio Grande do Sul - Departamento de Materiais, RS (Brazil)

    2010-07-01

    The electrode/membrane system, called MEA, is the fundamental unit of a PEMFC (proton exchange membrane fuel cell). Within the MEA, the gas diffusion layer (GDL) is the bridge between the flow field and the catalyst layer. One of the important elements in a GDL is the substrate, typically a carbon cloth or paper, that has to be an excellent electrical conductor and show mechanical strength along with thermal and chemical stability. In this work, GDLs were produced from a suspension containing short carbon fibers in water-based polyurethane and poly(vinyl alcohol) (PVA) resins with appropriate characteristics to be used in low temperature fuel cells. The obtained GDL was characterized regarding its wettability, electrical conductivity and morphological aspects, evaluated by SEM. (author)

  17. Low power laser effects in cancer cells and fibroblasts submitted the ionizing radiation; Efeitos do laser de baixa potencia em celulas de linhagem tumoral e fibroblastos submetidos a radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Camila Ramos

    2015-07-01

    Cancer is considered a public health problem worldwide. According to Brazil's the National Cancer Institute (INCA), 576,000 new cases of cancer were estimated for 2015 in Brazil, representing the second leading cause of death. Radiotherapy may be a treatment to several of types of cancer, frequently using ionizing radiation to eradicate or prevent the proliferation of tumor cells. This treatment, however, can lead to death of non-tumor cells around in irradiated tissue. Given this, adjuvant therapies that can minimize the side effects of ionizing radiation are of extremely importance. In this context, low power laser (LPL) may be an alternative to modulate the response of healthy cells to ionizing radiation. In this study, cells of human gingival fibroblasts (FMM1) and breast cancer (MDAMB- 231) were exposed to gamma radiation at doses of 2.5 and 10 Gy. After twenty-four hours, cell were irradiated with LPL ( λ= 660 nm, 40 mW and total area of 0.04 cm²) with energy densities of 30, 60, 90, 120 and 150 J/cm². The cell viability was measured during four days, using the trypan blue technique. The influence of LPL on the cell cycle and on expression of the nuclear antigen of cellular proliferation (PCNA) was evaluated by flow cytometry. The expression of β-Galactosidase was the chosen method to assess cell senescence. Considering our adopted parameters, and focusing on the non-tumor cells, we have observed an increase in: 1) cell viability; 2) cell population in phases S and G{sub 2}/M cell cycle; 3) PCNA expression with decrease in senescence. No alterations were observed in the cell viability, with greater population in phases S and G{sub 2}/M cell cycle, while the number of senescent cells and the expression of PCNA were decreased. Therefore, we have concluded that the LPL promoted effects on both cell lineages, with increased cell viability on FMM1 cells, whether cancer cells maintained a decreased proliferation. (author)

  18. Behavior of Electrochemically Prepared CuInSe{sub 2} as Photovoltaic Absorber in thin Film Solar Cells; Comportamiento del CuInSe{sub 2} Basado en Precursores Electrodepositados como Absorbente Fotovoltaico en Celulas Solares de Lamina Delgada

    Energy Technology Data Exchange (ETDEWEB)

    Guillen, C.; Martinez, M. A.; Dona, J. M.; Herrero, J.; Gutierrez, M. T. [Ciemat.Madrid (Spain)

    2000-07-01

    Two different objective have been pursued in the present investigation: (1) optimization of the CuInSe{sub 2} preparation parameters from electrodeposited precursors, and (2) evaluation of their photovoltaic behavior by preparing and enhancing Mo/CuInSe{sub 2}/CdS/TCO devices. When Cu-In-Se precursors are directly electrodeposited, the applied potential fit is essential to improve the photovoltaic performance. Suitable absorbers have been also obtained by evaporing an In layer onto electrodeposited Cu-Se precursors. In this case, the substrate temperature during evaporation determines the CuInSe{sub 2} quality. Similar results have been reached by substituting typical Mo-Coated glass substrates by flexible Mo foils. Different TCO tested (ZnO and ITO) have been found equivalent as front electrical contact in the devices. Solar cell performance can be improved by annealing in air at 200 degree centigree. (Author) 46 refs.

  19. PAPEL DEL INHIBIDOR DE LA VIA DE FACTOR TISULAR Y CAVEOLINA-1 SOBRE EL POTENCIAL INVASIVO Y LA ACTIVIDAD PROCOAGULANTE DE LAS CELULAS DE CANCER DE MAMA Y ENDOMETRIO.

    OpenAIRE

    HERNANDEZ BARRERA, SOLEDAD PAOLA

    2009-01-01

    El Factor tisular (FT) es una glicoproteína de transmembrana que inicia la vía extrínseca de la cascada de coagulación y actúa como receptor y cofactor enzimático del factor VII. Además el FT ha sido directamente relacionado con la agresividad del cáncer. 96p.

  20. Importance of gallium-67 scintigraphy in primary cutaneous B-cell lymphoma: report of two cases;Importancia da cintilografia com galio-67 no linfoma cutaneo primario de celulas B: relato de dois casos

    Energy Technology Data Exchange (ETDEWEB)

    Attab, Cyomara Sanches, E-mail: rubinha.attab@terra.com.b [Centro Brasileiro de Medicina Nuclear (CEBRAMEN), Goiania, GO (Brazil); Moriguchi, Sonia Marta [Fundacao Pio XII, Barretos, SP (Brazil). Hospital de Cancer; Paton, Eduardo Jose Alencar [Fundacao Pio XII, Barretos, SP (Brazil). Cancer Hospital. Div. of Hemotherapy and Bone-Marrow Transplant; Alencar, Mario Henrique Leite de [Clinica de Prevencao em Cancer, Goiania, GO, (Brazil); Rocha, Euclides Timoteo da [Fundacao Pio XII, Barretos, SP (Brazil). Cancer Hospital. Dept. of Nuclear Medicine

    2010-05-15

    The authors describe two cases of cutaneous B-cell lymphoma where correct staging, treatment and follow-up could be achieved through a combination of conventional imaging studies and gallium-67 scintigraphy. (author)

  1. P{sub N} solutions for the slowing-down and the cell calculation problems in plane geometry; Solucoes P{sub N} para os problemas da moderacao e do calculo de celula em geometria plana

    Energy Technology Data Exchange (ETDEWEB)

    Caldeira, Alexandre David

    1999-07-01

    In this work P{sub N} solutions for the slowing-down and cell problems in slab geometry are developed. To highlight the main contributions of this development, one can mention: the new particular solution developed for the P{sub N} method applied to the slowing-down problem in the multigroup model, originating a new class of polynomials denominated Chandrasekhar generalized polynomials; the treatment of a specific situation, known as a degeneracy, arising from a particularity in the group constants and the first application of the P{sub N} method, for arbitrary N, in criticality calculations at the cell level reported in literature. (author)

  2. Simplified process of manufacture of solar cells with 17% efficiencies using Cz substrata of low resistivity; Monitoramento do processo de fabricacao de celulas solares de silicio de alto rendimento, com passivacao posterior e utilizando silicio Cz de Baixa Resistividade

    Energy Technology Data Exchange (ETDEWEB)

    Stem, N.; Cid, M.; Ramos, C. A.S.; Chenlo, F.

    2008-07-01

    This work describes the fabrication process of solar cells with rear passivated surface using low resistivity Cz silicon of two different manufacturers. the PCD technique allowed to quantify the lifetime, about 0,25 ms (p=2.5{omega}.cm) and 0.35 ms (p=3.3.{omega}.cm) measured at the end of the process, and the implied open-circuit voltages of 652.5mV and 662.6mV, respectively. Efficiencies higher than 20% were predicted for an optical frontal system composed by random texturization with ZnS-MgF{sub 2}. (Author)

  3. Optimal design of a hybrid photovoltaic and fuel cell power generation system, to supply isolated communities in the Brazilian Amazon; Dimensionamento otimo de sistemas hibridos, com geracao fotovoltaica e celula a combustivel, para atendimento a comunidades isoladas na Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Sergio Batista da

    2010-11-15

    The lack of electricity in isolated communities in the Brazilian Amazon has become one of the greatest barrier for the development of the region. Currently, the main technologies that provide electricity to these communities are diesel generators, batteries and dry cells. These non-renewable energy sources may pose serious problems to the environment and human health and have high maintenance and operational costs. Therefore, the search for renewable energy sources, such as water and sunlight, which are highly abundant in the region, has become a great challenge. This thesis presents the studies on application of solar photovoltaic (PV) and fuel cell (FC) technologies to supply electric power in an uninterrupted manner. Outlined are the technical and cost issues of a pilot project set up in an environmentally protected area, next to Bananal island, located in the Southwestern region of the state of Tocantins. The pilot project relies on PV solar power as the primary source of energy for the production of electricity. The surplus energy is stored in the form of hydrogen produced by electrolysis of the water supplied locally, which is reconverted into electric power by fuel cells during periods when there is little or no sunlight. In this context, the aim of the study was to propose a sizing of a hybrid distributed generation system (HDGS), comprised of a PV system, FC and batteries, that optimizes implementation and operational costs, as a potential source of energy for isolated communities in the Amazon. The work was carried out with the help of simulation software HOMER (Hybrid Optimization Model for Electric Renewable) developed by National Renewable Energy Laboratory (NREL). Simulations and a comparative study were carried out of the technologies and potential configurations that meet the needs of these isolated communities. The results showed an optimal solution of HGDS PV-FC batteries with a reduction in the initial cost of the project in about 60% compared to the sizing system only. The HOMER simulated results also indicated that more than 50% of the costs of the PV-FC-battery HDGS regarded the PV system. (author)

  4. Influence of photon recycling effects in the operation and design of GaAs solar cells; Influencia del reciclaje de fotones en el funcionamiento y del diseno de las celulas solares de Arsenico de Galio

    Energy Technology Data Exchange (ETDEWEB)

    Balenzategui Manzanares, J. L.

    2005-07-01

    Photon recycling (PR) is the process by which photons internally emitted in a semiconductor can be re-absorbed by the material, giving as result new electron-hole pairs. Although this process has been receiving some international research from the Sixties, because their effects revealed as relevant in certain devices and materials (as in gallium arsenide), its influence in the operation of solar cells has been scarcely considered in the past. Thus deposited it has been demonstrated that one of its major effects is an enhancement of the radiative carrier lifetine, photon recycling is not usually taken into account in photovoltaic, neither in device modelling and simulation, nor from the perspective of taking advantage of the phenomenon to improve the efficiency of solar cells. This work describes the results of our investigations in the field of photon recycling. (Author)

  5. Study of the combined action of gamma radiation and static electric fields in human cells; Estudo da acao combinada de radiacao gama e campo eletrico estatico em celulas humanas

    Energy Technology Data Exchange (ETDEWEB)

    Moron, Michelle Mendes

    2008-07-01

    The basic principle of radiotherapy is the one of maximizing damage to the tumor, while minimizing it in neighboring health tissues. Several strategies have been worked out aiming at increasing cellular radiosensitivity, and among them is the use of exogenous fields. Our goal in this work is the study in human cells of the effect resulting from the association of irradiation with exposure to exogenous static electric fields. The T47D cell line of breast cancer cells was irradiated with gammas in the 0 - 8 Gy doses range. The corresponding survival curve provided information on the radiosensitivity of this cell line. The rate of cell deaths per Gray in the 0 - 8 Gy range exhibited a maximum at 2 Gy, which corresponds to the most efficient irradiation dose. The viability of this T47D cells exposed to both gamma radiation and 1.250 V/cm static electric field (SEF) was about 12% lower than when only irradiated. The sole exposure of the cells to SEF by 24 and 72 hours didn't induce toxicity. Immunofluorescence runs carried out in irradiated normal MRC5 cell line of human lung fibroblast, without and with exposition to a SEF, have quantified the expression of the y- H2AX histone. The amount of phosphorylated histones was approximately 40% higher after irradiation with 2 Gy plus exposure to a SEF by 1 hour, showing that the electric field negatively interfered in the repairing process of the DNA double strand breaks. The flow cytometry analysis with FACS allowed the investigation of a possible interference of radiation and SEF in the cell distributions among the cellular cycle phases. It was found that in T47D cells treated with 1 and 2 Gy by 24 hours the SEF also negatively interfered in the DNA repairing process, as evidenced by the higher accumulation of cells in the S phase. Therefore, it would be possible to conclude that static and exogenous electric fields are able of negatively interfering in the cellular repair and, presumably, in DNA repair. (author)

  6. Determination of the survival curve of the cell irradiated with low dose rate neutrons; Determinacao da curva de sobrevivencia de celulas irradiadas com neutrons a baixa taxa de dose

    Energy Technology Data Exchange (ETDEWEB)

    Mendes, Bruno M.; Freitas, Marcelo H.A. de; Campos, Tarcisio P.R. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear; Silva, Luciana M.; Dias, Consuelo L.F. [Fundacao Ezequiel Dias, Belo Horizonte, MG (Brazil). Dept. de Pesquisa. Lab. de Bioquimica

    2000-07-01

    The effect of the radiation in tissue and cells are a theme of extreme importance and that is receiving large attention more and more now. This work purposes to evaluate the deleterious effects of the neutrons radiation at low dose rate in lineages of human cancerous cells through the analysis of survival curves. Deleterious effects due to radiation of low dose rate (3.8 mSv/hr) were observed in cells of the lineage HeLa S3. Previous studies, in lineage HN5 of tongue carcinoma, showed survive level to 0.2Sv of 80%, compatible to the value of 88,57% 0.4 Sv obtained for HeLa S3. In a close future, with the molecular biology characterization of a great number or possibly all cancer types (that will be accelerated largely with the conclusion of the human genome project), studies like this, can be much more specific, and could determine the efficiency of the neutrons or any other radiation type for each one of those types. Those data would have of great value for the medicine and they would help to plan the treatments better. (author)

  7. Experimental study of a PEM 15 W fuel cell; Influencia das temperaturas de operacao e umidificacao no desempenho de uma celula combustivel tipo 'PEM' de 15 W

    Energy Technology Data Exchange (ETDEWEB)

    Heusi, Daniel S.; Steidel, Paulo S.; Matelli, Jose A. [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Lab. de Combustao e Engenharia de Sistemas Termicos (LabCET); Bazzo, Edson [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil)

    2004-07-01

    Experimental results are presented for a fuel cell type PEM of 15 W. The influence of the operating temperature, as well as the effect of the humidification temperature in the draining of the gases Hydrogen and oxygen, are considered for analysis. The tests were performed at operating temperature in the range of 30 to 70 deg C and humidification temperature form 30 to 85 deg C. For hydrogen flows fixed to be 200 ml/min and 300 ml/min, as well as for oxygen flow of 165 ml/min, the polarization curves were raised taking into account different combinations of operation and different combinations of humidification temperatures. (author)

  8. Study of a fuel cell insertion in a hybrid system for energy generation; Estudo da insercao de celula a combustivel integrada a sistema hibrido de geracao de eletricidade isolado

    Energy Technology Data Exchange (ETDEWEB)

    Vale, Silvio Bispo do; Bezerra, Ubiratan Holanda; Pinho, Joao Tavares; Pereira, Edinaldo Jose da Silva [Universidade Federal do Para (UFPA), Belem, PA (Brazil). Programa de Pos-Graduacao em Engenharia Eletrica. Grupo de Estudo e Desenvolvimento em Alternativas Energeticas

    2004-07-01

    This paper presents a case study of a fuel cell (FC) insertion in a small hybrid system for energy generation composed by wind (10 kW), photovoltaic (3,2 kW) and diesel (20 kVA) generations, making computer simulations in order to evaluate the contribution of each one of the energy-supply systems involved. This paper intends to contribute for the establishment of parameters to measure the technical and economic viability for using such isolated hybrid systems, trying in the future several combinations of models to hybridize the energy generation in Amazon isolated villages, making easier the use of local energy resources. (author)

  9. Development and testing of the proton exchange membrane fuel cell (PEMFC) for stationary generation; Desenvolvimento e ensaios de uma celula a combustivel de polimero solido (PEMFC) para geracao estacionaria

    Energy Technology Data Exchange (ETDEWEB)

    Ellern, Mara; Boccuzzi, Cyro Vicente [ELETROPAULO, Sao Caetano, SP (Brazil)], e-mail: mara.ellern@aes.com; Ett, Gerhard; Saiki, Gerson Yukio; Janolio, Gilberto [ELECTROCELL, Sao Paulo, SP (Brazil); Jardini, Jose Antonio [Universidade de Sao Paulo (USP), SP (Brazil)

    2004-07-01

    PEM (Proton Exchange Membrane) fuel cell uses a simple chemical reaction to combine hydrogen and oxygen into water, producing electric current in the process. It works something like reversed electrolysis: at the anode, hydrogen molecules give up electrons, forming hydrogen ions (this process is made possible by the platinum catalyst). The proton exchange membrane allows protons to flow through, but not electrons. As a result, the hydrogen ions flow directly through the proton exchange membrane to the cathode, while the electrons flow through an external circuit. As they travel to the cathode through the external circuit, the electrons produce electrical current. At the cathode, the electrons and hydrogen ions combine with oxygen to form water. In a fuel cell, hydrogen's natural tendency to oxidize and form water produces electricity and useful work. No pollution is produced and the only byproducts are water and heat. The huge advance on materials development combined with the growth demand of lower impact on environment is placing the fuel cells on the top of the most promising technologies world-wide. They are becoming in medium term feasible alternatives for energy generators up to energy plants of few MW. (author)

  10. Evaluation of the recombination in somatic cells induced by radiation in different stages of Drosophila larval development; Evaluacion de la recombinacion en celulas somaticas inducida por radiacion en diferentes etapas del desarrollo larvario de Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Cruces, M.P.; Morales R, P. [Instituto nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The mitotic recombination can happen spontaneously and its frequency is very low, however the recombination rate of a cell can be increased by the exposure to agents which cause damage to DNA. This type of agents are knew commonly as recombinogens. The ionizing radiation and a numerous chemical agents can be mentioned (Vogel, 1992). The objective of this work is to determine if the mutation/recombination rate induced by gamma rays varies with the development stage. In order to realize this investigation it was used the mutation and somatic recombination test of Drosophila wing (Graf and col. 1984). The mwh/ mwh and flr{sup 3}/TM3, Ser stocks were used. (Author)

  11. Action of the chlorophyllin before genetic damage induced by gamma radiation in germinal cells of Drosophila; Accion de la clorofilina ante el dano genetico inducido por radiacion gamma en celulas germinales de Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Moreno B, R

    2004-07-01

    The chlorophyllin (CHLN) is a porphyrin of nutritious grade and soluble in water, derived of the chlorophyll. It has been reported that this pigment is a good anti mutagen since it reduces the damage to the DNA caused by physical or chemical agents of direct or indirect action. Their anti carcinogenic action has also been demonstrated when it is administered itself during the induced post-initiation phase by aflatoxins and heterocyclic amines. However in the last decade it has been reported that it also has promoter activity against the genetic damage induced by diverse agents like the alkyl ants of direct and indirect action, the gamma radiation and some heterocyclic amines. This effect has been observed in testing systems like Salmonella, Drosophila, rainbow trout and rodents. In the mouse spermatogonia it has been reported that it reduces the damage to the DNA but with the test of lethal dominant in Drosophila increment the damage induced by gamma radiation. The present study consisted on evaluating the effect of the CHLN in the line germinal masculine of Drosophila by means of the lethal recessive test bound to the sex (LRLS) with the stump Muller 5 and a litters system. Its were pretreated wild males with CHLN and 24 h later were irradiated with 0, 10, 20 and 40 Gy of gamma radiation immediately later were crossed with virgin females of the stump Basc and at 72 h the male was transferred to a cultivation media with three new virgin females, this process repeated three times until completing 3 litters. The F1 it was crossed among itself and in the F2 it was analysed the presence or absence of lethals. The results indicated that the CHLN per se incremented the basal frequency of damage due to the pigment can act as an agent that is inserted to the ADN causing pre mutagenic leisure. Nevertheless with the groups treated with the different doses of gamma radiation the CHLN does not present any protector action, neither promoter except in the litter I of the group treated with 20 Gy where it was observed a promoter effect of genetic damage. (Author)

  12. Action of the chlorophyllin on the genetic damage induced by gamma radiation in germinal cells of Drosophila Melanogaster; Accion de la clorofilina sobre el dano genetico inducido por radiacion gamma en celulas germinales de Drosophila Melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Cruces, M.P.; Pimentel, A.E.; Moreno, A.; Moreno, R. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2003-07-01

    The obtained results using somatic cells, they have evidenced that the chlorophyllin (CHLN) it can act inhibiting or increasing the damage caused by different mutagens. The objective of this investigation is to evaluate the effect of the CHLN on the damage induced by gamma radiation in germinal cells of Drosophila. Two tests were used, the lost of the X chromosome and the conventional test of lethal recessive bound to the sex (LRLS); both with a system of litters. The obtained results in both essays, indicated that the CHLN doesn't reduce the damage induced by the gamma radiation in none of the cellular monitored states. (Author)

  13. Action of the chlorophyllin (CHLN) on the double breaking induced by gamma radiation in germinal cells of Drosophila melanogaster; Accion de la clorofilina (CHLN) sobre los dobles rompimientos inducidos por radiacion gamma en celulas germinales de Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Moreno M, A

    2005-07-01

    The chlorophyllin (CHLN) is a derived of the chlorophyll in the one which the atom of Mg is replaced by Cu. It has been broadly used as preservative in those foods, and in the treatment of geriatric patients. The results using different test systems have demonstrated that the CHLN reduces the DNA damage caused by different physical agents or chemical of direct action or insinuation. Another of the properties of the CHLN is it anti carcinogenic action, because it has been that inhibits the carcinogen activity of B1 (AFB1) aflatoxin and it diminishes the incidence of tumors caused for 2-amine-3-methylimidazo [4- 5f] quinoline (IQ) and it inhibits the development of colon cancer during the post-initiation phase. Recently the reports of the activity promoter of the CHLN have been increased on the genetic damage. This effect observed in Salmonella and later on in Drosophila melanogaster using, physical and chemical agents. Presently study determines the action of the CHLN before the genetic effect induced in germinal cells of Drosophila melanogaster by means of the test of the lost one of the X chromosome in ring using two protocols; the first one consisted on pretreatment with CHLN to the male ones and later on to irradiate them and in the second protocol the pretreatment with CHLN administers to the females, in both protocols its were used a litter systems. (Author)

  14. Study of a hybrid system using solid oxide fuel cells (SOFC) and gas turbine; Estudo de um sistema hibrido empregando celula de combustivel de oxido solido (SOFC) e turbina a gas

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Antonio Carlos Caetano de; Gallo, Giulliano Batelochi; Silveira, Jose Luz [UNESP, Guaratingueta, SP (Brazil). Faculdade de Engenharia. Dept. de Energia], e-mail: caetano@feg.unesp.br

    2004-07-01

    In this paper a hybrid solid oxide fuel cell (SOFC) system, applying a combined cycle using gas turbine for rational decentralized energy production is analyzed. The relative concepts about the fuel cell are presented, followed by some chemical and technical information such as the change of Gibbs free energy in isothermal fuel oxidation directly into electricity. This represents a very high fraction of the lower heating value (LHV) of a hydrocarbon fuel. In the next step a methodology for the study of SOFC and gas turbine system is developed, considering the electricity and steam production for a hospital. This methodology is applied to energetic analysis. Natural gas is considered as a fuel. A Sankey Diagram shows that the hybrid SOFC system is a good opportunity to strengthen the decentralized energy production in Brazil. It is necessary to consider that the cogeneration in this version also is a good technical alternative, demanding special methods of design, equipment selection and contractual deals associated to electricity and fuel supply. (author)

  15. Vitrification of nanotoxic waste (Ru) from the production of nano-catalysts for direct ethanol fuel cells; Vitrificacao de nano-residuos toxicos (Ru) provenientes da producao de nano-catalisadores para celulas a combustivel de etanol direto

    Energy Technology Data Exchange (ETDEWEB)

    Silva, A.C.; Julio-Junior, O.; Mello-Castanho, S.R.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2010-07-01

    Nanostructured catalysts have been developed for ethanol directly use in fuel cells, which due to the economic advantages that should have widespread use in the near future. The catalysts for these devices using nano-structured metal are based, where the toxic nature and environmental risks presented by these metals are largely enhanced by nano-dispersion. Thus, the production of nano-catalysts are potentially generating highly hazardous waste for public health and the environment. This study presents the treatment and inertization of ruthenium (Ru) nanoparticles waste containing by the vitrification technique and consequent attainment of silicate glasses for potential commercial use. Compositions were prepared containing up to about 20 wt % of nano-waste by changing the basic composition of glass soda-lime-borosilicate. After the fusion, at a temperature of 1100 deg C, the glasses were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Infra-red in the Fourier transform (FT-IR) techniques. The chemical stability was evaluated by hydrolytic attack test. The glass containing 20 wt % of nano-residue showed a high chemical stability, similar to a usual soda-lime glass. (author)

  16. Processing of strontium-doped lanthanum manganite suspensions for cathode production of the solid oxide fuel cell; Processamento das suspensoes de manganito de lantanio dopado com estroncio para fabricacao do catodo da celula a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Chiba, R.; Vargas, R.A.; Andreoli, M.; Seo, E.S.M. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Ciencia e Tecnologia de Materiais. Lab. de SOFC - Insumos e Componentes

    2008-07-01

    The ceramic material, strontium-doped lanthanum manganite (La{sub 0,85}Sr{sub 0,15}MnO{sub 3} - LSM), has been used as cathode in Solid Oxide Fuel Cells (SOFCs). The cathode attainment as component of the SOFCs has been studied for diverse routes of synthesis and thin films forming in Yttria-stabilized zirconia (ZrO{sub 2}/Y{sub 2}O{sub 3} - YSZ) electrolyte. In this work, the LSM was synthesized by the citrate technique and deposited in YSZ substrate using the forming technique wet powder spraying. Rheological studies of suspensions and chemical, physical and microstructural characterizations of LSM powders were made, aiming at the deposition for thin films formation until 50 mum. The half unit cells LSM/YSZ sintered were characterized by scanning electron microscopy, for verification of porosity and adherence. In this sense, this work is a contribution for production of porous cathode using the forming technique wet powder spraying in the SOFCs. (author)

  17. Development of a computational model applied to a unitary 144 CM{sup 2} proton exchange membrane fuel cell; Desenvolvimento de um modelo numerico computacional aplicado a uma celula a combustivel unitaria de 144 CM{sup 2} tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Robalinho, Eric

    2009-07-01

    This work presents the development of a numerical computer model and methodology to study and design polymeric exchange membrane fuel cell - PEM. For the validation of experimental results, a sequence of routines, appropriate to fit the data obtained in the laboratory, was described. At the computational implementation it was created a new strategy of coupling two 3-dimensional models to satisfy the requirements of the comprehensive model of the fuel cell, including its various geometries and materials, as well as the various physical and chemical processes simulated. To effective assessment of the real cell analogy with numerical model, numerical studies were carried out. Comparisons with values obtained in the literature, characterization of variables through laboratory experiments and estimates from models already tested in the literature were also performed. Regarding the experimental part, a prototype of a fuel cell unit of 144 cm{sup 2} of geometric area was designed, produced and operated at laboratory with the purpose of validating the numerical computer model proposed, with positive results. The results of simulations for the 2D and 3D geometries proposed are presented in the form of polarization curves, highlighting the catalytic layer model based on the geometry of agglomerates. Parametric and sensitivity studies are presented to illustrate the change in performance of the fuel cell studied. The final model is robust and useful as a tool for design and optimization of PEM type fuel cells in a wide range of operating conditions. (author)

  18. Analysis and development of an ethanol compact reformer for hydrogen production for fuel cell; Analise e modelagem de reformador compacto de etanol para obtencao de hidrodenio para celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Figueroa, P.R.F.; Oliveira, A.A.M. [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica. Lab. de Combustao e Engenharia de Sistemas Termicos], e-mail: renzo@labcet.ufsc.br, e-mail: amirol@emc.ufsc.br

    2006-07-01

    The objective of this work is to analyze the ethanol steam reforming for hydrogen production in a compact and modular reforming unit designed for the generation of 1 kw of electrical power. For this, initially the thermodynamic limits for the steam reforming of ethanol are calculated in order to assess the limits in the production of hydrogen and other by-products and to select the best values of process stoichiometry, temperature and pressure for maximum hydrogen selectivity and minimum coke formation. In the following, a First and second Laws analysis is performed to analyze the equilibrium conditions of the main chemical reactions and to estimate the magnitude of the heat transfer required by the heating, evaporation, superheating and reforming of ethanol. Then, the catalytic reformer reactor is analyzed and sized, basing the analysis into the application of the equation for the conservation of mass of the chemical species and a model for the chemical kinetics. A basic reactor design is then proposed accompanied by the corresponding sizes and operating conditions. (author)

  19. A Hull cell study of a NiW electrolyte and evaluation of its properties; Estudio en celula Hull de un bano electrolitico de NiW y evaluacion de sus propiedades

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Urrutia, I.; Diez, J. A.; Muller, C.; Calvillo, P.

    2009-07-01

    Interest in NiW coatings has grown in recent years due to its favourable properties such as hardness, and resistance to both wear and corrosion, making it one of the actual alternatives to hard chromium coatings. In this work, we have undertaken a Hull cell study, investigating the influence of the metal concentration, temperature, pH and current density on the composition of the alloy formed and its thickness. We have also studied the most important properties of the NiW deposits, including morphology, hardness, and resistance to abrasion and corrosion. (Author) 9 refs.

  20. Uncertainty of efficiency of a fuel cell with the establishing of suitable instrumentation for his measurement; Incerteza da eficiencia de uma celula a combustivel com o estabelecimento da instrumentacao adequada para a sua medicao

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Sergio Pinheiro de; Trota Filho, Jorge; Couto, Paulo Roberto Guimaraes; Rocha, Adriana da Cunha [Instituto Nacional de Metrologia Normalizacao e Qualidade Industrial (INMETRO), Duque de Caxias, RJ (Brazil)]. E-mail: spoliveira@inmetro.gov.br

    2008-07-01

    This work presents and compares the results obtained from the assessment of the uncertainty of measurement of the energetic efficiency calculations of a PEM unit fuel cell and from the total electrical power of a PEM fuel cell stack. This assessment makes use of three different methods of calculation: the Classical method and the Kragten method, both described in the ISO GUM 95 'Guide to the expression of uncertainty in measurement' and the Monte Carlo method, described in the Supplement 1 to the ISO GUM 95. Concerning the energetic efficiency, both the Classical and Kragten methods presented the same uncertainty values, 0.00029 whereas the Monte Carlo method presented a value of 0.00021. The difference between these two values (0.8 x 10{sup -5}) was greater than the {delta} criteria tolerance established in the Supplement 1 (0.5 x 10{sup -5}). This fact though, has not invalidated the results of the ISO GUM 95, as this work focused on the conservative value of the uncertainty. With respect to the uncertainty of the total electrical power of the fuel cell stack it has been observed that the main contribution for the uncertainty calculation, i.e., the higher uncertainty source (92.8%), was given by the accuracy class of the multimeter employed in the measurements. Additionally to the comparative study of the methods for the uncertainty calculations, this work establishes the correct accuracy class of a multimeter used for the measurement of the given/consumed electric potential by a fuel cell stack, aiming the cost/benefit relation of the process. (author)

  1. Performance of a solid oxide fuel cell with cathode containing a functional layer of LSM/YSZ film; Desempenho de uma celula a combustivel de oxido solido com catodo contendo uma camada funcional de filme LSM/YSZ

    Energy Technology Data Exchange (ETDEWEB)

    Pires, Filipe Oliveira; Domingues, Rosana Z.; Brant, Marcia C.; Silva, Charles L.; Matencio, Tulio [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Quimica]. E-mail: filipequiufmg@ufmg.br

    2008-07-01

    Performance of a SOFC may be evaluated by using the AC-Impedance and measuring power (P V x I). The objective of this study was to compare the performance of a fuel cell with LSM as a cathode and another one containing an additional functional composite film LSM/YSZ between the LSM and YSZ. Also it was studied variation in second cell resistance and power according to the temperature, hydrogen flux and operation time. For both cells platinum was used as anode. At 800 deg C was observed, in open current circuit, when the composite layer was introduced a decrease in resistance and high power. These results show an improvement of SOFC cathode performance with the introduction of composite LSM/YSZ layer. The maximum performance of the cell was achieved with 100 mL/min hydrogen flow at 800 deg C. The experiments also showed a performance improvement at 850 deg C. The cell behavior was stable during 318 hours of test. (author)

  2. Performance evaluation of a fuel cell with NiO-YSV anode operating with natural gas; Avaliacao do desempenho de uma celula a combustivel com anodo de NiO YSZ operando com gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Nobrega, Shayenne Diniz da; Vasconcelos, Carmel Suzarte Ayres; Lima, Luiz Rogerio Pinho de Andrade [Universidade Federal da Bahia (UFBa), Salvador, BA (Brazil). Escola Politecnica. Dept. de Ciencia e Tecnologia dos Materiais]. E-mail: shayennedn@yahoo.com.br

    2008-07-01

    Fuel cell is an electrochemical device that converts the chemical energy into electric energy. The natural gas, for its proven improvement in the income of the equipment in relation to other energy ones, has been very used to feed the solid oxide fuel cell (SOFC) in the generation of electric power. Ceramics of Yttria-stabilized zirconia had been used as electrolyte and when supported with nickel oxide they act as anode in the solid oxide fuel cell, due to raised ionic conductivity that these materials present in high temperatures, while lanthanum with strontium and manganite are used as cathode. In the composition of the anode, the concentration of Ni O, acting as catalytic in the YSZ confers high electric conductivity and high electrochemical activity of the reactions, providing the internal reform in the SOFC. In this work, the solid oxide fuel cell, formed by Yttria- stabilized zirconia, nickel oxide, and lanthanum with strontium and manganite were tested in the reform had been prepared samples of electrode/electrolyte for use in SOFC of the natural gas in the presence of low water text, similar condition to the operation of the SOFC, operating in temperatures range from 700 to 800 deg C. This cell also was characterized using the impedance spectroscopy technique. These results allowed the development of components of the current versus voltage. (author)

  3. Effect of {sup 60} Co gamma radiation on trehalose level of Saccharomyces boulardii cells; Efeito da radiacao gama {sup 60} Co nos niveis de trealose de celulas de Saccharomyces boulardii

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Raquel Gouvea dos; Neves, Maria Jose [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil). Nucleo de Radiobiologia; Duarte, Rinaldo; Nicoli, Jacques R. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Microbiologia

    1996-07-01

    The medicine Floratil used for diarrhea treatment is composed by lyophilized yeast cells of Saccharomyces boulardii. The action mechanism of this product is unknown. The efficacy of S. boulardii depends on its viability. The increase in the trehalose level in S. cerevisiae cells submitted to a previous sub-lethal level heat shock results in resistance to a lethal shock. The aim of this work was to study weather the cell trehalose level is involved also in the resistance of gamma irradiation. It was noted that the yeast cells surviving to gamma irradiation had more trehalose level than control cells. Apparently there is a positive correlation between of trehalose level and gamma irradiation resistance of S.boulardii cells. (author)

  4. Study of internalization and viability of multimodal nanoparticles for labeling of human umbilical cord mesenchymal stem cells; Estudo de internalizacao e viabilidade de nanoparticulas multimodal para marcacao de celulas-tronco mesenquimais de cordao umbilical humano

    Energy Technology Data Exchange (ETDEWEB)

    Miyaki, Liza Aya Mabuchi [Faculdade de Enfermagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Sibov, Tatiana Tais; Pavon, Lorena Favaro; Mamani, Javier Bustamante; Gamarra, Lionel Fernel, E-mail: tatianats@einstein.br [Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil)

    2012-04-15

    Objective: To analyze multimodal magnetic nanoparticles-Rhodamine B in culture media for cell labeling, and to establish a study of multimodal magnetic nanoparticles-Rhodamine B detection at labeled cells evaluating they viability at concentrations of 10 {mu}g Fe/mL and 100{mu}g Fe/mL. Methods: We performed the analysis of stability of multimodal magnetic nanoparticles-Rhodamine B in different culture media; the mesenchymal stem cells labeling with multimodal magnetic nanoparticles-Rhodamine B; the intracellular detection of multimodal magnetic nanoparticles-Rhodamine B in mesenchymal stem cells, and assessment of the viability of labeled cells by kinetic proliferation. Results: The stability analysis showed that multimodal magnetic nanoparticles-Rhodamine B had good stability in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium. The mesenchymal stem cell with multimodal magnetic nanoparticles-Rhodamine B described location of intracellular nanoparticles, which were shown as blue granules co-localized in fluorescent clusters, thus characterizing magnetic and fluorescent properties of multimodal magnetic nanoparticles Rhodamine B. Conclusion: The stability of multimodal magnetic nanoparticles-Rhodamine B found in cultured Dulbecco's Modified Eagle's-Low Glucose medium and RPMI 1640 medium assured intracellular mesenchymal stem cells labeling. This cell labeling did not affect viability of labeled mesenchymal stem cells since they continued to proliferate for five days. (author)

  5. Synthesis of yttria-doped zirconia anodes and calcium-doped ceria electrolyte to fuel cell; Sintese de anodos de zirconia dopada com itria e eletrolito de ceria dopada com calcia para celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, G.R.S de; Fagury Neto, E.; Rabelo, A.A., E-mail: grs_gustavo@hotmail.co [Universidade Federal do Para (UFPA), Maraba, PA (Brazil). Fac. de Engenharia de Materiais

    2010-07-01

    From the pursuit of lower operating temperature of fuel cells solid oxide was used polymeric precursor for the synthesis of reactive powder compositions Zr{sub 0,92}Y{sub 0,08}O{sub 2} for the anode and Ce{sub 0,88}Ca{sub 0,12}O{sub 2} for the electrolyte. The solutions were prepared using the metal in much of the composition and citric acid molar ratio of 1:3, under stirring at 60 deg C/1 h. The mixture of metallic citrates was subjected to agitation at a temperature of 80 deg C which was added ethylene glycol in the ratio 60:40 by weight citric acid / ethylene glycol, to form a resin that was pre-calcined at 300 deg C/3 h for to form the expanded resin. The powders were disaggregated in a mortar, screened and calcined at 400, 600 and 800 deg C/2 h. The powders were characterized by standard X-ray diffraction. (author)

  6. Development of solar cells with back surface field made by aluminum paste and belt furnace diffusion; Desenvolvimento de celulas solares com campo retrodifusor formado por pasta de aluminio e difusao em forno de esteira

    Energy Technology Data Exchange (ETDEWEB)

    Marcolino, Juliane Bernardes

    2011-01-15

    Photovoltaics is based on the direct conversion of solar energy into electricity and is a promising alternative to diversify the world's energy matrix. This work aims to develop and analyse the deposition of Al paste by screen printing and firing/diffusion in a belt furnace to produce a BSF region in monocrystalline Si wafers. The diffusion of Al into the substrate was implemented by two different processes. In the first process the diffusion/firing of the Al paste and the firing of the Ag paste was carried out in independent steps. In this case, solar cells with an average efficiency ({eta}{sub average}) of 11.5 % and a maximum of 12.0 % were produced, but with the formation Al clusters in the back surface of the devices. In the second process firing/diffusion of such pastes was done on the same step. In this case, the best results were obtained for a firing/diffusion temperature of 860 deg C and belt furnace speed (V{sub E}) of 150 cm/min and also for 890 deg C and 180 cm/min. For the former parameters, {eta}{sub average} was 12.4 % and the maximum was 12.8 %. For the later, {eta}{sub average} was 12.5 % and the maximum was 12.6 %. Considering a temperature of 900 deg C and V{sub E} of 190 cm/min, {eta}{sub average} was 12.4 %. It was observed that minority carriers diffusion lengths were smaller than the thickness of silicon wafers. Open circuit voltages were 30 mV lower than that from similar cells fabricated at NT-Solar by using high purity Al deposited by e-beam evaporation indicating that the developed process produced low quality BSF. (author)

  7. Stimulation of murine stem cell proliferation by circulating activities produced during the recovery of a radiation-induced hematopoietic injury. Estimulacion proliferativa de celulas madre hematopoyeticas de raton por actividades circulantes producidas durante la recuperacion de un dano hematopoyetico radioinducido

    Energy Technology Data Exchange (ETDEWEB)

    Grande Azanedo, M.T.

    1989-02-01

    The proliferative activity of CFU-S, low in normal steady state, increases after treatment with different aggressors, i.e., radiation. This stimulation has been attributed in part to a local regulation system of stem cell proliferation, and at least in part to a humoral regulatory system. In the present work it has been investigated the role that circulating activities have in the CFU-S stimulation, by means of in vitro and in vivo incubation assays with diffusion chambers. The results show that bone marrow of mice irradiated with 5 Gy produces in vitro diffusible activities capable of stimulating the CFU-S proliferation. As well with this same dose circulating activities are also produced in vivo. In addition we have observed that these activities are only released during the periods of active hematopoietic regeneration that follow irradiation with moderate doses (1.5 and 5 Gy). In another set of experiments we saw that the stimulating activities are also detected in serum of mice irradiated with 5 Gy. These serum activities modify the proliferative state of very primitive precursors (12 d CFU-S). When the serum activities are added to long term bone marrow cultures the CFU-S are also stimulated to proliferate. Finally, we observed that the radiation-induced serum activities stimulate the proliferation of bone marrow CFU-S when injected into normal mice, suggesting that such activities are involved in the regulation of CFU-S proliferation.

  8. Development of a computational model applied to a unitary 144 cm{sup 2} proton exchange membrane fuel cell; Desenvolvimento de um modelo numerico computacional aplicado a uma celula a combustivel unitaria de 144 CM{sup 2} tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Robalinho, Eric

    2009-07-01

    This work presents the development of a numerical computer model and methodology to study and design polymeric exchange membrane fuel cell - PEM. For the validation of experimental results, a sequence of routines, appropriate to fit the data obtained in the laboratory, was described. At the computational implementation it was created a new strategy of coupling two 3-dimensional models to satisfy the requirements of the comprehensive model of the fuel cell, including its various geometries and materials, as well as the various physical and chemical processes simulated. To effective assessment of the real cell analogy with numerical model, numerical studies were carried out. Comparisons with values obtained in the literature, characterization of variables through laboratory experiments and estimates from models already tested in the literature were also performed. Regarding the experimental part, a prototype of a fuel cell unit of 144 cm of geometric area was designed, produced and operated at laboratory with the purpose of validating the numerical computer model proposed, with positive results. The results of simulations for the 2D and 3D geometries proposed are presented in the form of polarization curves, highlighting the catalytic layer model based on the geometry of agglomerates. Parametric and sensitivity studies are presented to illustrate the change in performance of the fuel cell studied. The final model is robust and useful as a tool for design and optimization of PEM type fuel cells in a wide range of operating conditions. (author)

  9. Obtaining of ceria - samaria - gadolinia ceramics for application as solid oxide fuel cell (SOFC) electrolyte; Obtencao de ceramicas de ceria - samaria - gadolinia para aplicacao como eletrolito em celulas a combustivel de oxido solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Arakaki, Alexander Rodrigo

    2010-07-01

    Cerium oxide (CeO{sub 2}) when doped with rare earth oxides has its ionic conductivity enhanced, enabling its use as electrolyte for Intermediate Temperature Solid Oxide Fuel Cell (IT-SOFC), which is operated in temperatures between 500 e 700 degree C. The most effective additives or dopants for ionic conductivity improvement are (samarium oxide - Sm{sub 2}O{sub 3}) and gadolinia (gadolinium oxide - Gd{sub 2}O{sub 3}), fixing the concentration between 10 and 20 molar%. In this work, Ce{sub 0,8}(SmGd){sub 0,2}O{sub 1,9} powders have been synthesized by hydroxide, carbonate and oxalate coprecipitation routes. The hydrothermal treatment has been studied for powders precipitated with ammonium hydroxide. A concentrate of rare earths containing 90wt% of CeO{sub 2} and other containing 51% of Sm{sub 2}O{sub 3} and 30% of Gd{sub 2}O{sub 3}, both prepared from monazite processing, were used as starting materials. These concentrates were used due the lower cost compared to pure commercial materials and the chemical similarity of others rare earth elements. Initially, the coprecipitation and calcination conditions were defined. The process efficiency was verified by ceramic sinterability evaluation. The results showed that powders calcined in the range of 450 and 800 degree C presented high specific surface area (90 - 150 m{sup 2}.g{sup -1}) and fluorite cubic structure, indicating the solid solution formation. It was observed, by scanning electron microscopy, that morphology of particles and agglomerates is a function of precipitant agent. The dilatometric analysis indicated the higher rate of shrinkage at temperatures around 1300-1350 degree C. High densification values (>95% TD) was obtained at temperatures above 1400 degree C. Synthesis by hydroxides coprecipitation followed by hydrothermal treatment demonstrated to be a promising route for crystallization of ceria nano powders at low temperatures (200 degree C). High values of specific surface area were reached with the employment of hydrothermal treatment (about 100 m{sup 2}.g{sup -1}). High density ceramics were obtained at lower temperatures (1400 degree C), compared to those employed for calcined powders. (author)

  10. Transitional cell carcinoma arising from a bladder diverticulum: computed tomography findings - case report; Carcinoma de celulas transicionais em um diverticulo de bexiga: achados na tomografia computadorizada - relato de caso

    Energy Technology Data Exchange (ETDEWEB)

    Almeida Junior, Jose Guiomar de; Santos, Maria Lucia de Oliveira; Vabo, Telmo Pimentel do; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Radiologia]. E-mail: edmarchiori@zipmail.com.br; Torres Neto, Gilberto [Hospital Universitario Pedro Ernesto, Rio de Janeiro, RJ (Brazil). Dept. de Radiologia; Silva, Klezer Gaspar Carvalho da [Hospital de Clinicas de Niteroi, RJ (Brazil)

    2003-03-01

    We report the case of a patient with a transitional cell carcinoma arising from a bladder diverticulum that was diagnosed by computed tomography. Although there is a greater incidence of neoplasms in bladder diverticula, its occurrence is uncommon and the diagnosis is at times difficult. The role, the importance and limitations of computed tomography in the diagnosis of transitional cell carcinoma arising from a bladder diverticulum are discussed. (author)

  11. Development and scale-up of the production process of NovoCell fuel cells; Desenvolvimento e 'scale-up' do processo de producao de celulas a combustivel NovoCell

    Energy Technology Data Exchange (ETDEWEB)

    Azevedo, Dayse Caldas de; Souza, Adler de; Ferreira, Valdemar Stelita [NovoCell Sistemas de Energia S.A., Santa Barbara D' Oeste, SP (Brazil)]. E-mail: dayse.azevedo@novocell.ind.br

    2008-07-01

    Fuel cells present the potentiality to substitute the engines of internal combustion in vehicles and to supply energy for stationary use. This potentiality, however, not yet reflected in its introduction in the market with regular lines of production, because of its high cost and lack of criteria that demonstrate its reliability and durability. These subjects are the main goals of the programs of development of fuel cells worldwide. NovoCell is a Brazilian company whose objective is to develop and to produce hydrogen/air fuel cells for stationary generation. All the project is guided by the use of technologies/processes and materials that allow production in large scale and to a competitive cost, giving support to a continuous program of innovation and development of the product. In this work the technological solutions developed by the company are presented. (author)

  12. Acid membranes of poly(vinyl alcohol) for direct ethanol fuel cell applications; Membranes acidas de poli(alcool vinilico) para aplicacoes em celulas a combustivel via etanol direto

    Energy Technology Data Exchange (ETDEWEB)

    Dutra Filho, Jose C.; Gomes, Ailton S. [Instituto de Macromoleculas Professora Eloisa Mano, Universidade Federal do Rio de Janeiro, RJ (Brazil)], e-mail: asgomes@ima.ufrj.br

    2011-07-01

    Proton-conducting hybrid membranes composed of poly(vinyl alcohol) (PVA), phosphotungstic acid (HPW) and diethylenetriaminepentaacetic acid (DTPA) where prepared. The effect of HPW doping and crosslink with DTPA on the membranes properties such as uptake, pervaporation and proton conductivity was investigated. Uptake and permeated flux decreases with increasing content of HPW and DTPA. Ethanol permeabilities obtained was about two orders of magnitude smaller than Nafion 117. FTIR spectra indicated that HPW was incorporated into the polymer matrix and DTPA acted as crosslink agent. The proton conductivity was in the order of 10-3 S.cm-1 with added 4 wt.% of DTPA and generally increases with the addition of HPW. (author)

  13. Comparative morphocytologic study of native and irradiated crotoxin on CBA/J mice tissues and cells; Estudo morfocitologico comparativo de crotoxina nativa e irradiada em tecidos e celulas de camundongos CBA/J

    Energy Technology Data Exchange (ETDEWEB)

    Cardi, Bruno A.

    1995-12-31

    Snake venoms submitted to gamma radiation are better immunogens, have less toxic activity and maintain immunogenic and antigenic properties, favoring its use in anti-sera production which is the main therapy in human accidents. Severe human accidents attributed to neurotoxicity, are caused by Crotalus durissus terrificus, which main toxin - crotoxin - acts on neuro muscular junctions, as detected by electrophysiological studies and could be detoxified by gamma radiation. In order to evaluate the biological effects of this irradiated protein, we analyzed, at morphological and immunohistochemical levels, organs and cells from CBA/J mice challenged with crude venom or purified crotoxin, in an acute intoxication model. Both toxins were submitted to 2 kGy irradiation with a Co-60 source, in water solution. (author). 140 refs., 10 figs., 1 tab.

  14. Stable expression of human thyrotropin (hTSH) in mammalian cells (CHO) expressing {alpha}2,6 sialyltransferase; Expressao estavel tireotrofina humana (r-hTSH) em celulas de mamifero (CHO) que expressam {alpha}2,6 sialiltransferase

    Energy Technology Data Exchange (ETDEWEB)

    Damiani, Renata

    2009-07-01

    A CHO cell line, previously genetically modified by the introduction of rat {alpha}2,6-sialyltransferase cDNA, generated for the first time a human-like sialylated recombinant hTSH (hlsr-hTSH) more similar to the native hormone, with 61% of {alpha}2,3- and 39% of {alpha}2,6-linked sialic acid residues. The best clone, when submitted to gene amplification with up to 8 {mu}M methotrexate, presented a secretion level of {approx}2 {mu}g hTSH/10{sup 6} cells/day, useful for product purification and characterization. The relative molecular masses (M{sub r}) of the heterodimer and of the {alpha}- and {beta}-subunits of purified hlsr-hTSH, determined by MALDI-TOF mass spectrometry, and the relative hydrophobicities, determined by RP-HPLC, were not remarkably different from those presented by two r-hTSH preparations secreted by normal CHO cells. Some differences were observed, though, in N-glycan composition, with more tri- and much more tetra-sialylated structures in hlsr-hTSH. When analyzed via an in vivo bioassay based on hTSH-induced T{sub 4} release in mice, hlsr-hTSH was shown to be equipotent (p > 0.05) with the commercial preparation of r-hTSH (Thyrogen), and 1.5-fold more potent than native hTSH (p < 0.001). (author)

  15. Optimization by simulation and development of solar cells with aluminium paste rear emitter and diffusion in conveyor furnace; Otimizacao por simulacao e desenvolvimento de celulas solares com emissor posterior formado por pasta de aluminio e difusao em forno de esteira

    Energy Technology Data Exchange (ETDEWEB)

    Mallmann, Ana Paula

    2011-01-15

    Photovoltaic solar energy is the direct conversion of solar energy into electricity and it has low impact to the environment during electric energy production. The main device of this technology is the solar cell and silicon is the substrate most used. The solar cells are electrically connected and encapsulated in order to form the photovoltaic module. The aims of this thesis are to optimize, develop and to analyse n{sup +}np{sup +} solar cells processed in n type Si-PV-FZ and with aluminum rear emitter formed in belt furnace. The optimization of solar cells by simulation is an important step before the device development. The software PC-1D and another program developed using Visual Basic language were used. Considering a metal grid formed by evaporation technique in vacuum ambient an efficiency of 16.8 % may be achieved. With screen printed grid, 15.8 % efficient solar cells were obtained. From the simulation results it was found that the screen printing metallization may become more viable than evaporation technique because there is low difference in the efficiency and the screen printing is a simpler technique. The experimental optimization of silicon wafers texture process resulted in reflectance of 12 %. This value is typical for monocrystalline silicon with textured surface. Experimental optimization of phosphorus front surface field shows a sheet resistance of (36 {+-} 4) {omega}/ for this region. This region was formed in a thermal step in a conventional furnace with POCl{sub 3}. It was found that after the phosphorus diffusion occurred gettering to specific temperature and time. It was verified that the minority carrier lifetime in the final of processing is similar to the initial value. The influence of steps sequence of front silver paste firing and rear diffusion/firing aluminium paste, of surface passivation and the influence of dry air flow during the aluminium paste diffusion/firing, of aluminium paste diffusion/firing temperature and of belt speed under the fabricated devices electric characteristics were analysed. The aluminum rear emitter n{sup +}np{sup +} silicon solar cells resulted in 29 efficiency up to 9,5 %. The best solar cells were processed under 900 deg C to aluminium paste diffusion/firing and belt speed of 140 cm/min. The efficiency is limited by low values of V{sub OC} and FF reached. It was also verified that the local rear emitter formation results in solar cells with higher efficiency than those with homogeneous emitter. (author)

  16. Microstructural development and characterization of lanthanum chromite-based ceramics to application in solid oxide fuel cells; Desenvolvimento microestrutural e caracterizacao de ceramicas a base de cromita de lantanio para aplicacao em celulas a combustivel de oxido solido

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, R.N.; Furtado, J.G. de M.; Soares, C.M.; Serra, E.T. [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)], e-mail: rnunes@cepel.br

    2006-07-01

    This work has for objective to investigate and to characterize the microstructural development of lanthanum chromite-based ceramics (LaCrO{sub 3}) doped with earth alkaline metals, correlating the microstructural parameters (mainly the densification level) and processing parameters with the electrothermal properties reached. Lanthanum chromite-based ceramic systems doped with earth-alkaline metals (Ca, Mg and Sr) had been produced from respective metallic nitrates by solid state reactions process. The phase compositions were evaluated by X-ray diffraction and the densification level by Archimedes method. The microstructural characterization was effected by scanning electron microscopy, energy dispersive X-ray spectroscopy and thermal analysis techniques. Electrical tests were used to evaluate the electrical conductivity of the studied ceramics. The obtained results corroborate the literature comments concerning the difficulty of lanthanum chromite-based ceramics with high densification level and evidence the great influence of the nature of the dopants on the sintering mechanism and the microstructural and electric characteristics of the produced ceramics. The best ones results, in terms of densification and electrical conductivity, had been gotten through multiple doping with calcium and strontium, and in sintering temperature conditions lower that the normally considered to pure or monodoped lanthanum chromite-based ceramics. (author)

  17. Synthesis and characterization of sulfonic acid membranes based on interpenetrating polymer networks for application in fuel cells; Sintese e caracterizacao de membranas sulfonadas baseadas em redes polimericas interpenetrantes para aplicacao em celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Blanco, Lyzed Toloza; Loureiro, Felipe A.M.; Rocco, Ana Maria [Grupo de Materiais Condutores e Energia, Escola de Quimica, Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil)], e-mail: amrocco@eq.ufrj.br; Pereira, Robson Pacheco [Instituto de Ciencias Exatas, Universidade Federal Fluminense (UFF), Volta Redonda, RJ (Brazil)

    2011-07-01

    In the present work, the synthesis and characterization of sulfonic membranes based on interpenetrating polymer networks (IPN). In order to obtain such systems, the diglycidyl ether of bisphenol A (DGEBA) was polymerized in presence of polyethyleneimine (PEI). These membranes were submitted to sulfonation reactions, originating IPN-SO{sub 3}H membranes. The characterization by FTIR evidenced the formation of a Semi-IPN structure, while sulfonation reactions resulted in systems containing -SO{sub 3}H groups covalently bonded to the chains. The membranes exhibited water retention up to 200 degree C, in a temperature range sufficient for application in PEMFC under hydration. (author)

  18. Absence of genotoxic activity from milk and water boiled in microwave oven in somatic cells from Drosophila melanogaster; Ausencia da atividade genotoxica do leite e agua, fervidos com microondas, em celulas somaticas de Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Dias, Cristina das Dores. E-mail: crisddias@yahoo.com.br

    2003-07-01

    This paper reports an experiment for evaluation of the possible genotoxic effects of food prepared in a microwave oven, through the mutation test and somatic recombination, in wings of Drosophila melanogaster. Two crossing have been performed: a standard cross-ST and a high bioactivation cross - HB resulting in marked trans -heterozygote descendents (MH) and balanced heterozygotes (BH). The 72 hours larvas were fed with water and milk boiled both in the microwave oven and in the traditional way. The MH individual wings were analyzed, where the spots can be induced either by mutation or mitotic recombination. The experiment presented negative results related to the genotoxic effects of the water and milk boiled using the microwave oven, in MH descendents of both crossing. Therefore, under these experimental conditions, genotoxic activity were not presented by milk and water boiled in the microwave oven. However, an extensive study using different techniques is necessary to investigate the action of the food prepared in the microwave oven on the genetic material.

  19. Polycrystalline materials by chemical-bath-deposition for their application in thin film solar cells; Materiales policristalino depositados por bano quimico alternativos al sulfuro de cadmio para su aplicacion en celulas solares de lamina delgada

    Energy Technology Data Exchange (ETDEWEB)

    Bayon Cabeza, M. R. [Ciemat. Madrid (Spain)

    2000-07-01

    In the last years, chemical-bath-deposition (CBD) has been proven to be an excellent method for preparing sulphide and selenide-based thin film semiconductors. due to their optoelectronic properties, some of those materials can be used in thin film solar cells either as absorbers or as buffer-window-layers. In this way, the chemical-bath-deposition seems to be the best method for preparing the cadmium sulphide-buffer layer which is present in the thin film solar cells based on chalcopyrite absorbers (CIS or CIGS) because the highest efficiencies (about 19%) have been achieved for devices using buffer layer. Nowadays there has been considerable interest in replacing this compound, said toxic, by other CBD-semiconductors with similar or even better properties than the cadmium sulphide and, if possible, less health hazardous and more environmental friendly. Among all the alternative materials that have been studied, both the indium and the zinc hydroxisulphide have produced the best performance devices leading to efficiencies higher than 14%. Despite the good results obtained using those materials, only a few studies have been found in relation to the chemical bath process, the growth mechanism and the structural, optoelectronic and morphological properties of the thin films. Therefore the aim of this work is to prepare CBD-indium, indium-zinc and indium-cadmium hydroxisulphide thin films in order to study dependence of the film properties on the deposition conditions and to prove the suitability of those films for being used as alternative buffer layers in thin film solar cells. (Author)

  20. Experimental energetic analysis of gas natural-powered fuel cell cogeneration plant; Analise energetica experimental de uma planta de co-geracao com celulas a combustivel e gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Furtado, Jose G.M.; Lopes, Francisco C.; Silva Junior, Fernando R.; Soares, Guilherme F.W.; Serra, Eduardo T. [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)

    2008-07-01

    Power systems based on fuel cells have been considered for residential and commercial applications in energy Distributed Generation (DG) market as these systems can minimize their acquisition, installation and operation high costs. In this work we present an experimental analysis of a power generation system formed by a 5 kW proton exchange membrane fuel cell unit and a natural gas reformer (fuel processor) for hydrogen production, of the CEPEL's Fuel Cell Laboratory. It was determined the electrical performance of the cogeneration system in function of the design and operational power plant parameters. Additionally, it was verified the influence of the activation conditions of the fuel cell electrocatalytic system on the system performance. It also appeared that the use of hydrogen produced from the natural gas catalytic reforming provided the system operation in excellent electrothermal stability conditions resulting in increase of the energy conversion efficiency and of the economy of the cogeneration power plant. The maximum electrical efficiency achieved was around 38% and in all power range unit operated with average potential per single fuel cell higher than 0.60 V. (author)

  1. Development of electrode-membrane-electrode assemblies for proton exchange membrane fuel cells (PEMFC) by Sieve printing; Desenvolvimento de conjuntos eletrodo-membrana-eletrodo para celulas a combustivel a membrana trocadora de protons (PEMFC) por impressao a tela

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Alexandre Bodart de

    2008-07-01

    The Sieve Printing process was studied in this work to apply the catalyst layers onto electrolytes utilized in PEMFC. Initially, 25 cm{sup 2} active area MEAs were built for comparison with others MEAs produced by the Spray technique. The two methods produced MEAs that showed current densities higher than 600 mA.cm{sup -2} at 600 mV. A scaling up study for 144 cm{sup 2} of active area MEAs was carried out. For this purpose, a new cell had to be projected for shelter the MEAs in such dimensions. The profile of the gas distribution channels was developed through the computational fluid dynamic tool 'Comsol Multiphysics'. For the design of the bipolar plates of the cell the 'Auto CAD' was used. The 144 cm{sup 2} MEAs made by Spray and by Sieve Printing methods were confronted with commercials MEAs ones of equal dimensions. These commercials MEAs presented better performance at 600 mV, however they were more costly than the solution developed in this study. The new method was showed to be adequate to fabricate low cost MEAs of different geometries and to produce any amount of MEAs for small scale stacks (up to 10 kW). (author)

  2. Electric energy supply to isolated residences from the network through low power fuel cells; Fronecimento de energia eletrica a residencias isoladas da rede atraves de celulas a combustivel de baixa potencia

    Energy Technology Data Exchange (ETDEWEB)

    Serra, Eduardo Torres; Furtado, Jose Geraldo de Melo [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)], e-mail: etserra@cepel.br, e-mail: furtado@cepel.br

    2008-07-01

    This paper discusses the technical and socio economical aspects of the utilization of fuel cells for supplying electric power to isolated residences under an analysis on the global and brazilian energy scenarios, focusing the ascertaining of the high inequality of electric energy consumption among the various regions and countries in the world and the electric power access viability for the population lesser favoured. The paper performs an energetic analysis allowing an estimate of daily average consumption of electric energy of a low income family, and also discusses an attendance alternative of this demand by using small generation unities consisting of polymeric membranes fuel cells and reformers of primary fuel. Also, the paper analyses the possibilities of using various fuels for the hydrogen production to be used in fuel cell and estimates the cost of electric energy generated as function the main parameters which characterizes the enterprise.

  3. Thermodynamical simulation for solid oxide (SOFC) type fuel cells with ethanol direct internal reforming; Simulacao termodinamica para celulas a combustivel do tipo SOFC com reforma interna direta do etanol

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Aline Lima da; Malfatti, Celia de Fraga; Heck, Nestor Cezar [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Engenharia de Minas, Metalurgica e de Materiais (PPGEM)]. E-mail: als14br2000@yahoo.com.br; Mello, Celso Gustavo [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Engenharia Quimica (PPGEQ); Halmenschlager, Cibele Melo [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Engenharia de Minas, Metalurgica e de Materiais (PPGEM). Lab. de Materiais Ceramicos

    2008-07-01

    In SOFC, high operative temperature allows the direct conversion of ethanol into H{sub 2} to take place in the electrochemical cell. Direct internal reforming of ethanol, however, can produce undesirable products that diminish system efficiency and, in the case of carbon deposition over the anode, may occur the breakdown of the electrode. In this way, thermodynamic analysis is fundamental to predict the product distribution as well as the conditions favorable for carbon to precipitate inside the cell. Equilibrium determinations are performed by the Gibbs energy minimization method, using the GRG algorithm. Thermodynamic conditions for carbon deposition were analyzed, in order to establish temperature ranges and H{sub 2}O/ethanol ratios where carbon precipitation is not feasible. A mathematical relationship between Lagrange multipliers and carbon activity is presented, unveiling the carbon activity in atmosphere. The effect of the type of solid electrolyte (O{sup 2-} or H{sup +} conducting) on carbon formation is also investigated. The results of this work are in agreement with previous results reported in literature using the stoichiometric method. (author)

  4. Hydrogen production through allothermal ethanol reforming for fuel cells application: first generation prototype; Producao de hidrogenio atraves da reforma-vapor do etanol para aplicacoes em celulas a combustivel: prototipo de primeira geracao

    Energy Technology Data Exchange (ETDEWEB)

    Marin Neto, Antonio Jose; Silva, Ennio Peres da; Camargo, Joao Carlos; Neves Junior, Newton Pimenta; Pinto, Cristiano da Silva [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Lab. de Hidrogenio; Pinto, Cristiano da Silva [Centro Nacional de Referencia em Energia do Hidrogenio, Campinas, SP (Brazil)

    2004-07-01

    This paper describes preliminary results obtained with the allothermal ethanol reforming system for synthesis gas (syn-gas) production and hydrogen upgrading and purification for fuel cell applications. The system was designed to supply hydrogen to a 500 W PEM (Proton Exchange Membrane) fuel cell, with an electrical efficiency of 45%, which requires approximately 0.45 m3.h-1 of hydrogen, with a maximum carbon monoxide concentration of 20 {mu}mol.mol-1 (ppm). The study was performed changing the operation temperature and analyzing the resulting syn-gas through gas chromatography for a specific catalyst. This catalyst was tested up to 700 deg C, 1 bar and fixed stoichiometric steam to carbon ratio. The syn-gas, before carbon monoxide shift reactor implementation, was submitted to a two-bed-three-segments purification step composed of chemical and physical molecular sieves for hydrogen purification. The carbon monoxide shift reactor (water gas shift reactor) is under development to improve the efficiency-to-hydrogen and maximize the life of the purification bed. The final results also include a discussion about possible reactions involved in ethanol steam-reforming for such catalyst. (author)

  5. Energetic-economical analysis of a stationary for energy generation with fuel cells and natural gas reforming; Analise energetico-economica de um sistema estacionario de geracao de energia com celulas a combustivel e reforma de gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Furtado, Jose Geraldo de Melo; Silva Junior, Fernando Rodrigues; Silva, Cristiane Abrantes da; Soares, Guilherme Fleury Wanderley; Lopes, Francisco da Costa; Serra, Eduardo Torres [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)]. E-mail: furtado@cepel.br; Codeceira Neto, Alcides [Companhia HidroEletrica do Sao Francisco (CHESF), Recife, PE (Brazil)

    2008-07-01

    Power systems based on fuel cells have been considered for residential and commercial applications in energy Distributed Generation (DG) market as these systems can minimize their acquisition, installation and operation high costs. In this work we present an experimental analysis of a power generation system formed by a 5 kW proton exchange membrane fuel cell unit and a natural gas reformer (fuel processor) for hydrogen production, of the CEPEL's Fuel Cell Laboratory. It was determined the electrical performance of the cogeneration system in function of the design and operational power plant parameters. Additionally, it was verified the influence of the activation conditions of the fuel cell electrocatalytic system on the system performance. It also appeared that the use of hydrogen produced from the natural gas catalytic reforming provided the system operation in excellent electrothermal stability conditions resulting in increase of the energy conversion efficiency and of the economicity of the cogeneration power plant. The maximum electrical efficiency achieved was around 38% and in all power range unit operated with average potential per single fuel cell higher than 0.60 V. (author)

  6. Preparation, characterization and evaluation of electrocatalysts supported on functionalized carbon black for polymer exchange membrane fuel cell applications; Preparacao, caracterizacao e avaliacao de eletrocatalisadores suportados em carbono funcionalizado para aplicacao em celulas a combustivel tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Carmo, Marcelo do

    2008-12-18

    The fuel cell technology associated with the growing exigency of low environmental impact energy became prosperous in the world energy scenery. The fuel cell is basically a device that converts directly the chemical energy of a fuel into electrical and thermal energy with a continuous operation by the constant feed of a fuel. Especially, the carbon black Vulcan XC72 is usually employed as an electro catalyst support, and some factors as an accessible and high surface area in order to get maximum particles dispersion, pore size, adequate pore distribution and the presence of functional groups in the carbon black surface are considered fundamental characteristics for an innovative materials development. However, the Vulcan XC72 still reveals insufficient conditions for these purposes. This study consists in the preparation and in the physical chemical characterization of functionalized carbon black by hydrogen peroxide and by polymeric chains with proton conduction properties, and its posterior utilization as electro catalyst support for PEMFC and DMFC application. After the carbon functionalization, an improvement in the carbon black dispersion in water media was observed, a beneficial effect for electro catalyst preparation. It was also observed, that the functional groups and the polymeric chains worked as stabilizers in the particle growing, producing much more homogeneous electrocatalysts, exhibiting smaller average particle size. Especially, in the case of polymeric chains functionalization, a decrease in the ohmic drop was observed for this system, attributed to an improvement in the proton transference. (author)

  7. Environmental analysis of the proton exchange membrane fuel cell on the subject of life cycle assessment; Analise ambiental da celula a combustivel de membrana trocadora de protons sob o enfoque da avaliacao do ciclo de vida

    Energy Technology Data Exchange (ETDEWEB)

    Fukurozaki, Sandra Harumi

    2006-07-01

    The energy is the fuel of growth and an essential requirement for the socioeconomic development. However, the current production model is based on fossil fuels, considered as threat to man and nature. As for, the relating to the human activities and their effects on the environment, they are handled by the implementation of a more rigid model of environmental control and the mobilization of the society in favor of technologies with less energy impact. In view of this scenario, the Proton Exchange Membrane Fuel Cell - PEMFC has been recognized as a key for the vital need of a clean and efficient energy. Considering the conventional power generation system, their advantages during usage configure its application as an ideal option for several utilities, especially in the mobile sector. Even though, the focus on several environmental evaluations in energy systems is referred back to the initial stage of it use, the employment relating to production of the system and to final destination should be considered, since these also present impacts. In the case of PEMFC, their previous and subsequent phases of use are issues related to the platinum catalysts, which indicates an environmental importance that cannot be overlooked. In this sense, the Life Cycle Assessment has been used to understand and to question the risks and opportunities that are associated to certain product, starting from a systemic concept of their relationships with the environment. It is precisely in this context that the present research intends to present its major contribution, starting from an exploratory study towards the its objectives to provide an environmental analysis of such technology linked to post stage of powder-use of the membrane electrode assembly - MEA, concerning the platinum catalysts, on the subject of Life Cycle Assessment - LCA. To attain such aim, the relationships between energy, environment and development are presented and discussed, as well as, the Fuel Cell technology and the current studies on LCA of PEMFC. Several questions raised up on this issues have contributed in the development of a method of recuperating the PEMFC catalysts and, particularly, for its subsequent environmental evaluation. Among significant results are the importance of LCA, out lined as useful tool for perceiving the weight of environmental matters concerning the platinum and its subsidy strategies relating to the development, consolidation and to the innovation of PEMFC. (author)

  8. Polymeric membranes obtained from S-PEEK for application in PEM fuel cells; Caracterizacao de membranas polimericas obtidas a partir dos S-PEEK para aplicacao em celulas combustiveis do tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Barreto, Ednardo G.; Fiuza, Raildo A.; Catao, Ronei S.; Jose, Nadia M.; Boaventura, Jaime S. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Quimica], e-mail: ednardobarreto@yahoo.com.br, e-mail: raildofiuza@gmail.com, e-mail: roneicatao@ig.com.br, e-mail: nadia@ufba.br, e-mail: bventura@ufba.br; Pepe, Yuri [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Fisica

    2007-07-01

    This work had the objective to develop and to characterize S-PEEK membranes (sulfonated poly ether ether ketone) through chemical and electrochemical analyses. Conductivity test in function of the frequency and tension had been carried through; as well as, the open circuit tension of a fuel cell using the S-PEEK as electrolyte. Additional tests included TGA (Thermogravimetric Analysis), water absorption test, DSC (Differential Scanning Calorimetry), as tools to characterize conducting, thermal and mechanical proprieties of polymeric membrane. (author)

  9. Nepfix: on-demand cloud elastic simulation of networks of evolutionary processors

    OpenAIRE

    2015-01-01

    Una Red de Procesadores Evolutivos o NEP (por sus siglas en ingles), es un modelo computacional inspirado por el modelo evolutivo de las celulas, específicamente por las reglas de multiplicación de las mismas. Esta inspiración hace que el modelo sea una abstracción sintactica de la manipulation de information de las celulas. En particu¬lar, una NEP define una maquina de cómputo teorica capaz de resolver problemas NP completos de manera eficiente en tóerminos de tiempo. En la praóctica, se esp...

  10. "Discovering the Cell": An Educational Game about Cell and Molecular Biology

    Science.gov (United States)

    Spiegel, Carolina N.; Alves, Gutemberg G.; Cardona, Tania da S.; Melim, Leandra M. C.; Luz, Mauricio R. M. P.; Araujo-Jorge, Tania C.; Henriques-Pons, Andrea

    2008-01-01

    The role of games within education becomes clearer as students become more active and are able to take decisions, solve problems and react to the results of those decisions. The educational board game "Discovering the Cell" ("Celula Adentro"), is based on problem-solving learning. This investigative game attempts to stimulate…

  11. The RISC (Reduced Instruction Set Computer) Architecture and Computer Performance Evaluation.

    Science.gov (United States)

    1986-03-01

    8 )1; II;-21(82 ? 1JV , *~A 1*-e eo I Q .f ’ . - . - . .> - Approved for public release; distribution is unlimited. The RISC Architecture and Computer...1000 Lisboa Portugal 6. Manuel Pedrosa de Barros 4 Celula 5 Bloco 5 Lote D, 3 Direito 2795 Linda-a-Velha Portugal t~m " 96" ..... ...... |f

  12. Opioid system manipulation during testicular development: results on sperm production and sertoli cells population = Manipulação do sistema opioidérgico durante o desenvolvimento testicular: consequência sobre a produção espermática e a população de células de sertoli

    Directory of Open Access Journals (Sweden)

    Fernanda Mafra Cajú

    2011-04-01

    Full Text Available The Sertoli cell has fundamental importance to the development andmaintenance of spermatogenesis, as well as it has a directly proportional numerical relationship to sperm production. The proliferative period of this cell in rats occurs between 13 days pre-natal and 21 days pos-natal, when is established the final population in adult animals. The Leydig cell can modulate the Sertoli cell proliferation during fetal and neonatal periodƒn throughƒnƒnƒÒ-endorphin. The manipulation of opioidergic system can promote changes in parameters related to development of nervous, endocrine andreproductive systems. By the way, the main purpose of this present work was to compare the effects of the blockade of opioid receptor blocking in Sertoli cells using naltrexone (50 mg kg-1 during fetal and neonatal period in Wistar rats. According to the results, themanipulation of opioidergic system during pre-natal period reduced the total length of seminiferous tubule and Sertoli cell population in adult rats, but sperm production was normal because this cell has had a compensatory response for spermatozoids support capacity.As celulas de Sertoli tem fundamental importancia para o desenvolvimento e manutencao da espermatogenese, bem como possuem uma relacao numerica diretamente proporcional com a producao espermatica. O periodo proliferativo destas celulas em ratos ocorre entre 13 dias pre-natal e 21 dias pos-natal, resultando na definicao da populacao decelulas de Sertoli nos animais adultos. As celulas de Leydig podem modular a proliferacao das celulas de Sertoli durante o periodo fetal e neonatal por meio da ƒÒ-endorfina. A manipulacao do sistema opioidergico durante esta fase pode promover alteracoes em parametros relacionados com o desenvolvimento dos sistemas nervoso, endocrino ereprodutivo. Em virtude disto, o objetivo do presente trabalho foi comparar os efeitos do bloqueio de receptores opioides nas celulas de Sertoli, utilizando o naltrexone (50 mg kg

  13. [Prognostic implications of folliculo-stellate cells in pituitary adenomas: relationship with tumoral behavior].

    Science.gov (United States)

    Tortosa, F; Pires, M; Ortiz, S

    2016-10-01

    Introduccion. A pesar del progreso en la comprension de su patogenia, no se ha encontrado ningun marcador predictivo independiente del comportamiento agresivo de los adenomas hipofisarios que facilite el tratamiento y seguimiento de pacientes afectados. Objetivo. Analizar la expresion de celulas foliculo-estrelladas, mediante inmunomarcacion con proteina S-100, en una serie de pacientes con adenomas hipofisarios seguidos durante al menos siete años. Pacientes y metodos. Estudio retrospectivo de 51 pacientes diagnosticados de adenoma hipofisario entre 2006 y 2008, segun los criterios vigentes de la Organizacion Mundial de la Salud. Se evaluo inmunohistoquimicamente la expresion de S-100 en celulas foliculo-estrelladas, y se correlaciono con parametros clinicorradiologicos e histopatologicos del tumor y la progresion/recurrencia postoperatoria. Resultados. De 51 tumores, 40 se clasificaron como adenomas hipofisarios tipicos y 11 como atipicos. La mayoria de los tipicos mostro celulas foliculo-estrelladas positivas para S-100 (media: 3,93%); los atipicos tenian pocas o ninguna celula S-100 positivas (media: 0,83%). No hubo diferencias significativas en la expresion de S-100 con respecto a la edad o sexo del paciente, tamaño, invasividad o recidiva tumoral posquirurgica. Conclusiones. En el grupo de estudio, a excepcion de los adenomas no funcionantes inmunopositivos para prolactina, con la media mas baja y mas alta de todos los subtipos en ambos grupos (tipicos, 0,25%, frente a atipicos, 9,24%; p = 0,0028), el factor predictivo de agresividad tumoral para los adenomas hipofisarios no esta representado por un bajo valor de S-100 en las celulas foliculo-estrelladas, lo que no permite seleccionar a pacientes para un tratamiento postoperatorio intensivo.

  14. Establecimiento de una metodología para la caracterización de herramientas de corte para el taladrado estructuras híbridas

    OpenAIRE

    Torres Marchán, Pablo

    2015-01-01

    Establecimiento de una metodología para la caracterización de herramientas de corte para el taladrado estructuras hibridas. Localización del desgaste en la herramienta de corte utilizada en el taladrado de aluminio-fibra de carbono.

  15. Facilitating Subject Matter Expert (SME)-Built Knowledge Bases (KBS)

    Science.gov (United States)

    2004-12-01

    2.85 43 35 150 42.53% 111 102 52.11% 13 11.71% SRI SME Amoeba 2.29 30 30 364 62.28% 601 1292 31.75% 95 15.81% SRI SME Celula 1.68 25 25 554 27.77...Amoeba 0 0 2 2 5 86 SRI SME Celula 0 0 1 69 28 59 SRI SME Iflu 0 0 0 7 53 51 SRI KE sriPN 0 0 3 186 67 57 SRI KE sriAS 0 1 6 97 57 81 SRI SME Vaccinia 0...utilized only the NuSketch sketching tool which may have resulted in less complete answers. D iagnostic Q uestion D Q 1 W hich avenue of approach is explo

  16. Respostas ao défice hídrico em feijão (Phaseolus vulgaris) e caupi (Vigna unguiculata), induzidas por óxido nítrico

    OpenAIRE

    2007-01-01

    O oxido nitrico (NO) atualmente e reconhecido como um mediador intra e intercelular de diversas funcoes em celulas animais e vegetais. Enquanto alguns autores consideram-no como agente indutor de estresse, outros afirmam que o mesmo possua papel protetor agindo principalmente, na interrupcao de cadeias de radicais livres formadas sob estresse oxidativo. Os fatores mais limitantes ao crescimento vegetal sao os estresses ambientais sendo a falta de agua, o mais adverso. Os efe...

  17. [In vitro effect of the S3Pvac vaccine against cysticercosis in human mononucleate cells].

    Science.gov (United States)

    Díaz-Orea, María Alicia; Mijares, José Miguel; Arcega, Raúl; Gómez-Conde, Eduardo; Castellanos-Sánchez, Víctor Omar; Briones-Rojas, Rosendo; Flores-Alonso, Juan Carlos; Marín-Briones, Miguel Ángel; Santos-López, Gerardo

    2013-05-01

    Introduccion. La neurocisticercosis (NCC) es una infeccion parasitaria generada por el establecimiento de cisticercos de Taenia solium en el sistema nervioso central. La fase larvaria del parasito tambien afecta al cerdo, que es el huesped intermediario indispensable para la transmision. Por tal motivo, muchos investigadores se han enfocado en identificar antigenos protectores para prevenir la cisticercosis porcina e interrumpir la transmision. Entre ellos figuran los antigenos de la vacuna S3Pvac, constituida por tres peptidos protectores: KETc1, KETc12 y GK1. Objetivo. Evaluar el efecto de los peptidos vacunales KETc1, KETc12 y GK1 en celulas mononucleares de pacientes con NCC e individuos sanos. Sujetos y metodos. Estudio comparativo, prospectivo y transversal. Se analizo la proliferacion y el perfil de citocinas inducidos por los tres peptidos en celulas mononucleares de tres pacientes con NCC activa, 16 pacientes con NCC calcificada y 16 sujetos sanos. Resultados. KETc1 induce bajos niveles de proliferacion en las celulas de los pacientes con NCC activa y controlada, tanto en linfocitos como en monocitos. KETc12 y GK-1 inducen niveles positivos de proliferacion de monocitos en sujetos sanos. Conclusiones. El peptido KETc1 podria usarse como coadyuvante en el tratamiento de los pacientes con NCC activa, ya que indujo una respuesta Th2; y el peptido GK1, como estimulador del monocito/macrofago en inmunizaciones con otras proteinas.

  18. [Pathogenesis of spinal cord injuries and mechanisms of repair induced by olfactory ensheathing cells].

    Science.gov (United States)

    Botero, Lucía; Gomez, Rosa Margarita; Chaparro, Orlando

    2013-05-16

    Introduccion. La lesion medular es un evento catastrofico, cuyas consecuencias persisten durante toda la vida del paciente. La investigacion en tratamiento se ha basado principalmente en el desarrollo de terapias que reduzcan la discapacidad, pero desde los anos noventa hay un avance significativo y se han probado varios trasplantes celulares en modelos animales de lesion medular, celulas de Schwann, astrocitos y celulas de la glia envolvente olfatoria (CGEO). Objetivo. Hacer un recuento detallado de la patogenia de la lesion medular primaria y secundaria y de los mecanismos por los cuales las CGEO inducirian sus posibles efectos regenerativos descritos en la bibliografia. Desarrollo. Despues del traumatismo, la lesion se desarrolla en dos fases, la primaria se caracteriza por las lesiones de compresion y la secundaria se produce por una serie de factores que se dan en paralelo y que incluyen factores vasculares, celulares, moleculares y formacion de cicatriz glial. La mayoria de los modelos de lesion medular y trasplante con CGEO han comunicado recuperacion funcional, remielinizacion y regeneracion axonal. Estas celulas ejercen su accion de manera indirecta a traves de la produccion de factores de crecimiento y de manera directa induciendo regeneracion neuronal, axonal y remielinizacion. Conclusiones. Las CGEO son una opcion terapeutica en pacientes con lesion medular debido a que inducen de modo directo o indirecto regeneracion neuronal, axonal, remielinizacion de axones, disminucion de cicatriz glial y otros efectos que conducen a la recuperacion funcional.

  19. Study of the oxygen reduction reaction using Pt-Rare earths (La, Ce, Er) electrocatalysts for application of PEM fuel cells; Estudo da reacao de reducao do oxigenio utilizando eletrocatalisadores a base de Pt-terras raras (La, Ce, Er) para aplicacao em celulas a combustivel tipo PEM

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Thiago Bueno

    2013-07-01

    The complexity of the oxygen reduction reaction (ORR) and its potential losses make it responsible for the most part of efficiency losses at the Fuel Cells. For this reaction the electrocatalyst witch is most appropriated and shows better performance is platinum, a noble metal that elevates the cost, raising barriers for Fuel Cells technology to enter the market. First this work focuses on reducing the amount of platinum used in the cathode, by being replaced by rare earths. The most common methods of synthesis involves a large amount of steps and this work proposed to prepare the electrocatalyst through a simpler way that would not take so many steps and time to be done. Using an ultrasound mixer the electrocatalyst was prepared mixing platinum supported on carbon black and the rare earths lanthanum, cerium and erbium oxides to be applied in a half-cell study of the ORR. The Koutecky-Levich plots shows that among the electrocatalysts prepared the Pt80Ce20/C had the catalytic activity close to the commercial BASF platinum on carbon black, suggesting that the reaction was taken by the 4-electron path. As found in some works in literature, among the rare earth used to study the ORR, cerium is the one witch shows the better performance because it is able to store and provide oxygen. This feature is of great interest for the ORR because this reaction is first order to the oxygen concentration. Results show that is possible to reduce the amount of platinum maintaining the same electrocatalyst activity. (author)

  20. Study of synthesis routes and processing of NiO-YSZ ceramic composite for use as anode in solid oxide fuel cell (SOFC); Estudo de rotas de sintese e processamento ceramico do composito NiO-YSZ para aplicacao como anodo em celulas a combustivel do tipo oxido solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Yoshito, Walter Kenji

    2011-07-01

    This study aim the definition of synthesis and ceramic processing conditions of the anodic component suitable for operation of SOFC, i.e, homogeneous distribution of NiO in YSZ matrix and porosity after reduction above 30%. The selected synthesis routes included the co-precipitation in ammonia media, mechanical mixing of powders and combustion reaction from nitrate salts. The characterization techniques of powders included the X-ray diffraction, scanning and transmission electron microscopy, laser diffraction, nitrogen gas adsorption technique (BET) and Helium pycnometry. The obtained results indicated that the loss of Ni{sup 2+} in co-precipitation process, due to the formation of complex [Ni(NH{sub 3}){sub n}]{sup 2+}, can be minimized by controlling the pH around 9.3, keeping the concentration of nickel cation in the solution to be precipitated around 0.1M. In the mechanical mixing method the best condition of powder dispersion, without differential sedimentation, was obtained for zeta potential values at pH around 8.0, fixing the dispersant concentration at 0.8%. For the combustion synthesis it was observed that when stoichiometric and twofold stoichiometric urea was used, amorphous phase was formed and a higher surface area was attained in the final products. Employing the fuel-rich solution condition, crystallization of the powder was observed and the relative intensity of reflections of XRD patterns increased with excess of fuel, due to increasing the reaction temperature. Sinterability studies of pellets prepared from powder synthesized by the three routes described above showed the temperature around 1300 deg C for maximum rate densification and porosity between 6.0 and 14%. Reduction results of the composites confirmed that the reduction kinetics occurs in two steps. The first one with a linear behavior and controlled by chemical reaction on the surface. The second reduction step is the reduction that is controlled by gas diffusion in micro pores, generated by reduction of nickel oxide, decreasing the rate of reduction. (author)

  1. Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line; Marcacao intracelular e processo de quantificacao por imagem por ressonancia magnetica utilizando nanoparticulas magneticas de oxido de ferro em celulas da linhagem C6 de glioma de rato

    Energy Technology Data Exchange (ETDEWEB)

    Mamani, Javier Bustamante; Pavon, Lorena Favaro; Sibov, Tatiana Tais; Rossan, Fabiana; Silveira, Paulo Henrique; Cardenas, Walter Humberto; Gamarra, Lionel Fernel, E-mail: javierbm@einstein.br [Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Miyaki, Liza Aya Mabuchi [Faculdade de Enfermagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Amaro Junior, Edson [Departamento de Diagnostico por Imagem e Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil)

    2012-04-15

    Objective: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. Methods: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T{sub 1} and T{sub 2} weighted phantom images. Results: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxactivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r{sub 1}=(6.1 +- 0.3) x10{sup -5} ms{sup -1}mL/{mu}g, r{sub 2}=(5.3 +- 0.1) x 10{sup -4} ms{sup -1}mL/{mu}g, with a ratio of r{sub 2} / r{sub 1}{approx_equal} 9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R{sub 1}and R{sub 2}) using a mathematical relationship. Conclusions: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r{sub 2} / r{sub 1}{approx_equal} 9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T{sub 2}-weighted imaging techniques. (author)

  2. Application of a method for the determination of yeast cell density to studies on the sedimentation of Saccharomyces cerevisiae in alcoholic fermentation broths; Aplicacao de metodo para a determinacao da densidade de celulas de leveduras a estudos sobre sedimentacao de Aaccharomyces cerecisiae em mostos de fermentacao alcoolica

    Energy Technology Data Exchange (ETDEWEB)

    Maia, Amazile B.R.A. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Engenharia. Dept. de Engenharia Quimica; Nelson, David Lee [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Dept. de Alimentos

    1993-09-01

    A technique that permitted the determination of the Saccharomyces cerevisiae cell density was developed. The average results (1,04 g/ml) attributed to the cells under the conditions of the effected tests, was applied to a previously developed mathematical model for predicting the clarification velocity of alcoholic fermentation broths in a sedimenter prototype designed for accelerating the sedimentation of cells. (author) 19 refs., 1 fig., 4 tabs.

  3. Life cycle assessment (LCA) methodology: importance in the integration of the fuel cell technology type PEMFC (proton exchange membrane fuel cells); Metodologia da analise de ciclo de vida: importancia na insercao da tecnologia de celula a combustivel do tipo PEMFC (membrana polimerica trocadora de protons)

    Energy Technology Data Exchange (ETDEWEB)

    Fukurozaki, Sandra Harumi; Seo, Emilia Satoshi Miyamaru [Instituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)). Centro de Ciencia e Tecnologia de Materiais], e-mail: shfukuro@ipen.br

    2004-07-01

    To improve the standard of society's quality of life, it is necessary to improve the quality of distributed energy and its inherent services within a sustainability process. Among different technological routes that produce more sustainable energy are the fuel cells - also known as combustible batteries. The Global Environment Facility (GEF) has identified the fuel cells as a potential technology to reduce, in the future, the effect of greenhouse gases in both developed and developing countries. Although there are various types of fuel cells, the most used technology for research studies on fuel cells is the Polymer Electrolyte Fuel Cells (FEMFC). However, economic issues - related to the high cost of the membrane's materials and of the catalysts of groups of platinum metals - are still some of the obstacles that need to be overcome for this technology to be more accessible. There are also socio-environmental aspects related to the impacts caused by the extraction, the use and the destination of these metals. Taking in consideration the challenges of complying with the demands of the market and the society as well as with the growing tendency of more rigid patterns of environmental control, the objective of the present work is to show the tool of environmental management - Life Cycle Assessment (LCA) - and its importance on the pursuit for socio-economic and environmental alternatives feasible to the recycling of the catalysts of platinum of the PEMFC. This way, it intends to collaborate to the progress of the knowledge about environmental and socio-economic subjects related to the productive process of the PEMFC. (author)

  4. Preparation and characterization of PVA/SSA membranes with Al{sub 2}O{sub 3} nanoparticles for fuel cell applications; Preparacao de caracterizacao de membranas de PVAL/SSA na presenca de nanoparticulas de Al{sub 2}O{sub 3} para aplicacao em celulas de combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Paula N.; Pires, Alfredo T.N. [Grupo de Estudo em Materiais Polimericos - POLIMAT - UFSC, Florianopolis, SC (Brazil); Catarino, Margarida; Brandao, Lucia; Tanaka, Alfredo; Mendes, Adelio [Faculdade de Engenharia da Universidade do Porto, Porto (Portugal)

    2011-07-01

    In the present study, PVA/SSA membranes were prepared with and without the addition of Al{sub 2}O{sub 3} nanoparticles. Sulfosuccinic acid (SSA) was used as the crosslinking agent. Membranes were prepared with different amounts of SSA (26, 43 and 55 wt.%) and with 5 and 10 wt.% of nanoparticles. Crosslinking was performed at 90 degree C during 1.5 h. Membranes were analyzed by infrared spectroscopy, thermal analysis, water absorption, ion exchange capacity (IEC) and proton conductivity. The results showed that control of the crosslinking conditions, IEC value, water absorption and polymer structure are of significant importance to obtain a set of properties suitable for application in proton exchange membrane fuel cells. (author)

  5. Resistance to the ionizing radiation in cells of human melanoma. Role of the antioxidant enzymes and of the free radicals of the oxygen; Resistencia a la radiacion ionizante en celulas de melanoma humano. Papel de las enzimas antioxidantes y de los radicales libres del oxigeno

    Energy Technology Data Exchange (ETDEWEB)

    Medina, V.; Cricco, G.; Massari, N.; Nunez, M.; Martin, G.; Mohanad, N.; Gutierrez, A.; Bergoc, R.; Rivera, E. [Laboratorio de Radioisotopos, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Buenos Aires (Argentina); Crescenti, E.; Croci, M. [Instituto de Inmunooncologia, Cordoba 3200, Buenos Aires (Argentina)]. e-mail: vmedina@ffyb.uba.ar

    2006-07-01

    The malignant melanoma is a highly aggressive and potentially lethal type of skin cancer. Previously we have reported that the cellular human lines of melanoma WM35 and M15 are resistant to the ionizing radiation (IR). The histamine (HA) although it has a regulator effect of the cellular proliferation in these lines, it is not capable of to modify the response to the IR like it makes with other malignant cellular lines. To investigate the bases of the radioresistance of the melanoma lines we have studied in the WM35 the production of free radicals of oxygen (ROS), the activity of the antioxidant enzymes and their modifications by action of the IR and of the HA. In studies in vitro the cells were treated with HA 10 {mu}M from 20 hs before being irradiated with a dose of 2 Gy (source {sup 137}Cs, dose rate 7.7 Gy/min). The ROS levels, superoxide anion (O{sub 2}{sup -}) and hydrogen peroxide (H{sub 2}O{sub 2}) its were measured by flow cytometry using fluorescent coloring and the activity of dismutase superoxide (SOD), Catalase (CAT) and Glutathion Peroxidase (GPx) its were determined by spectrophotometric techniques and the protein levels by Western blot. The results indicate that in the cells WM35 the HA increases the production of H{sub 2}O{sub 2} in 96% and it diminishes lightly (17%) the levels of O{sub 2}{sup -} . On the contrary, the IR diminishes the levels of H{sub 2}O-2 in 47% and it increases in 46% those of O{sub 2}{sup -}. In the irradiated cells the HA power the decrease of H{sub 2}O{sub 2} produced by the IR. The variation of the enzymes activity is coincident with these changes in the levels of ROS: the treatment with HA increases the activity of SOD and it diminishes that of CAT in cells without irradiating; on the other hand, in the irradiated cells the HA it diminishes the SOD significantly. On the base of these results we can conclude that the levels of H{sub 2}O{sub 2} are directly related with the sensitivity of the cells WM35 to the IR. The HA is able to modulate the activity of the antioxidant enzymes and the levels of ROS. The IR activates cellular mechanisms that take to the radioresistance of these cells and that its interfere and/modifying the biological response mediated by the HA. (Author)

  6. Induction of genetic recombination in the lambda bacteriophage by ultraviolet irradiation of the Escherichia Coli cells. III. Role of the ruvA and recN genes; Induccion de recombinacion genetica en el bacteriofago lambda por irradiacion ultravioleta de las celulas de Escherichia Coli. III. Papel de los genes ruvA and recN

    Energy Technology Data Exchange (ETDEWEB)

    Alcantara D, D. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    1987-05-15

    The objective of this work is to determine the paper of the genes ruvA and recN in the stimulation of the recombination of Lambda for UV irradiation of Escherichia Coli, taking into account that both genes are inducible, they belong to the group of genes that participate in the SOS response and that a deficiency in its expression reduces the capacity to repair and recombiner the DNA. (Author)

  7. Modifier action of the chlorophyllin of the mutagenesis induced by the ethyl-nitroso-urea (ENU) in germinal cells of Drosophila melanogaster; Accion modificadora de la clorofilina de la mutagenesis inducida por la etil-nitroso-urea (ENU) en celulas germinales de Drosophila melanogaster

    Energy Technology Data Exchange (ETDEWEB)

    Morales N, I

    2006-07-01

    The cupro-sodium chlorophyllin (CCS) it is a soluble porphyrin in water that it includes in its it structures a copper atom instead of the magnesium that has the chlorophyll. Diverse experiments have demonstrated that it possesses a potent activity, reducing or inhibiting, the DNA damage caused by physical and chemical agents of direct action or insinuation. Most of the knowledge about their anti genotoxic activity has been obtained using somatic cells of different organisms, on the other hand it is known very little of their effect in germinal cells. At the moment in the Drosophila laboratory of the ININ it is investigating the protective action of the CCS in germinal cells, with these studies has been observed that its administration to females that were crossed with males irradiated with 20 Gy of gamma radiation, promotes the induction of lethal dominant in the embryonic and post-embryonic states causing a diminution in the viability egg-adult. With the test of lethal recessive bound to the sex one has evidence that it increases the basal frequency of lethal recessive and it doesn't reduce those induced by radiation. In contrast, with the present investigation when the CCS was administered to males that later on were treated with ethyl-nitroso-urea (ENU) caused a reduction of the lethal frequency in all the monitored cellular states, but only it was significant in the post-meiotic cells. On the contrary, when the CCS was administered to the female ones and then they crossed with males treaties with ENU, it was observed a tendency to increase the lethal ones in all the cellular types. With both protocols the CCS caused a diminution of the sterility. The fact that the CCS has antagonistic activities, it deserves special attention to investigate with different protocols and systems, the conditions in that this pigment can work as a true antimutagenic and/or anti carcinogenic before being able to him to propose as a chemopreventor. (Author)

  8. Activación de celulas supresoras de la respuesta de hipersensibilidad tardía en ratones BALB/c infectados o vacunados con Leishmania mexicana pifanoi Activation of supressor cells of delayed hypersensitivity response in BALB/C mice infected or immunized with Leishmania mexicana pifanoi

    Directory of Open Access Journals (Sweden)

    Hilda A. Pérez

    1985-12-01

    Full Text Available Los ratones BALB/c inmunizados SC con promastigotes no viables de Leishmania mexicana pifanoi, desarrollaron respuestas de hipersensibilidad tardia (RHT contra el parásito. En contraposición, los ratones BALB/c infectados crónicamente con L.m. pifanoi y aquellos inmunizados IV con una dosis supraóptima de parásitos muertos, mostraron inhibición de la RHT. La supresión de la RHT en los animales infectados, estuvo precedida por un estado transitorio de inmunidad celular, manifestado durante la fase inicial del desarrollo de las lesiones (4-12 semanas. La inhibición de la RHT observada en los animales infectados o inmunizados con dosis supraóptimas, fue causada por un mecanismo activo, transferible a receptores singénicos a través de las células esplénicas de los donantes suprimidos. La población de células supresoras fue no adherente a "nylon", sensible al tratamiento con un suero anti-timocitos de ratón y C', y de acción específica sobre la RHT contra antígenos leishmánicos. Se propone que la ocurrencia de células T supresoras de la RHT en los animales infectados crónicamente o inmunizados con dosis supraóptimas, es causada por el exceso de carga antigénica. Esta última, en el caso de los animales infectados, secundaria a una falla primaria en el control inmunológico de la población parasitaria.A T suppressor cell population that specifically shut down delayed hypersensitivity responses (DHR to the parasite was found in both BALB/c mice chronically infected with Leishmania mexicana pifanoi and in naive mice which had received a single IV supraoptimal dose of killed parasites. At the early phase of infection mice exhibited a transitory state of cell-mediated immunity against the parasite that was abrogated when lesions reached their accelerated phase of growth. Results suggest that in both infected and high-dose immunized mice, the activation of T suppressor cells of DHR is related to antigen overload.

  9. Cost analysis of electrical power from an ethanol reformer and the fuel cell in the development of productive activities in the community Pico do Amor, MT, Brazil; Analise do custo da energia eletrica proveniente de um reformador de etanol e celula a combustivel no desenvolvimento de atividades produtivas na comunidade Pico do Amor/MT

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Davi Gabriel; Teixeira, Andre Frazao; Lopes, Daniel Gabriel; Cavaliero, Carla Kazue Nakao [Universidade Estadual de Campinas (DE/FEM/UNICAMP), SP (Brazil). Faculdade de Engenharia Mecanica. Departamento de Energia; Instituto Aqua Genesis, Campinas, SP (Brazil); Hytron, Campinas, SP (Brazil)

    2010-07-01

    This work has the objective to analyze the impact of the cost of from an ethane reformer / fuel cell in the family income considering the development of two productive activities selected by the community itself: the production and marketing of cassava flour and 'rapadura', a typical brazilian candy. The community energy demand was analyzed to achieve the results; estimated the energy cost from the implemented system and the money from the selling of the cassava flour and 'rapadura' produced with this electricity; the study of sensibility of the ethanol price in the electrical energy cost was done too, and the cassava flour and 'rapadura' in the family funds. From the results, it was verified that the electrical energy cost has a 16,4% impact in the family gross income and a net value around R$ 260,85/family, indicating that the community will have enough funds to pay for the energy and also will rise the amount of money for each family. Besides, the comparative analyze of the cost of the electricity from the ethanol/fuel cell reformer and photovoltaic systems shows that, considering only the maintenance and operation costs, the first one should be more attractive than the second one. (author)

  10. {beta} -carotene effect the induction of the sister chromatid exchanges (ICH) by gamma radiation in mouse radiosensibilized osseous marrow cells In vivo; Efecto del {beta}- caroteno la induccion de intercambios en las cromatidas hermanas (ICH) por radiacion gamma en celulas radiosensibilizadas de la medula osea de raton In vivo

    Energy Technology Data Exchange (ETDEWEB)

    Morales R, P.; Cruz V, V.L. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico). Dept. de Biologia

    1997-07-01

    The effect of {beta}- carotene over the ICH radioinduction in radiosensibilized with BrdU osseous marrow cells of mouse was determined In vivo. The treatment with 50 {mu}g {beta} carotene per se induces a significant increment in the ICH frequency and the pre or post-treatment with the same dose causes an additive effect in the ICH frequency produced by 0.62 Gy of gamma radiation. This implies that {beta}- carotene does not have radioprotective activity, under conditions which was developed this experiment. (Author)

  11. Electrolytic hydrogen production at off-peak consumption and his utilization as energy vector at peak consumption through use of fuel cells; Producao de hidrogenio eletrolitico nos horarios fora de ponta e sua utilizacao como vetor energetico nos horarios de ponta atraves do uso de celulas a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Gambetta, Francielle [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Faculdade de Engenharia Mecanica. Dept. de Energia]. E-mail: fgambetta@fem.unicamp.br; Silva, Ennio Peres da [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Fisica. Lab. de Hidrogenio

    2008-07-01

    Due the fact that consumers have different demand, depending on the pace of economic activities and their daily habits of consumption, there is a different electrical demand throughout the day. Demand, in general, is minimal during the morning and it grows with the move for hours to reach peak consumption between 18:00 and 22:00, then turning to fall. It is old the idea of storing electricity produced in off-peak for its consumption in peak hours. To store large amounts of electrical power is required its conversion into some other form of energy, since it is impossible to store electrical energy as such in large quantities. One way of storing electricity is in the form of hydrogen produced by electrolysis of water which then can be stored, purified and converted into electricity through fuel cells. When there is an increase in demand for electricity, there is a need to expand the supply of energy, which means investments in generation, transmission and distribution. This work was performed a preliminary analysis of the production of electrolytic hydrogen in hours of low demand and its use in the generation of electricity in peak hours, making use of the fuel cells, so that to a certain limit value of increased electricity demand, there is no need for expansion of supply of Foz do Iguacu/PR. Moreover, at this work, the ability to harness the heat generated in fuel cells for heating water in homes was considered. (author)

  12. Synthesis and evaluation in vitro in cancer cells AR42J of the radiopharmaceutical {sup 99m}Tc-Tyr{sup 3}-Octreotide-dendrimer similar of somatostatin; Sintesis y evaluacion in vitro en celulas de cancer AR42J del radiofarmaco {sup 99m}Tc-Tyr{sup 3}-Octreotido-dendrimero analogo de la somatostatina

    Energy Technology Data Exchange (ETDEWEB)

    Orocio R, E.

    2013-07-01

    The objective of this project was preparing a multimeric system through the conjugation of several molecules of the peptide Tyr{sup 3}-Octreotide to a dendrimer molecule based on Poly-amidoamine (PAMAM), as well as radiolabeled with {sup 99m}Tc and evaluating its behavior like new radiopharmaceutical similar of somatostatin. The dendrimer PAMAM generation 3.5 that possesses terminal groups of sodium carboxylate, was functionalized to peptide Tyr{sup 3}-Octreotide through a reaction of peptide coupling with HATU (hexafluorophosphate (V) of 1-oxide-3-(bis(dimethylamino)methylene)-3H-[1,2,3]triazole[4,5-b]pyridine) as activating agent of carboxylate groups using the Size Exclusion Chromatography (Sec) as purification method. The product was characterized by Ultraviolet visible spectrophotometry, Mid-infrared and Far-infrared, Elemental analysis, Energy dispersive X-ray spectroscopy, Scanning electron microscopy, Thermogravimetry and Differential scanning calorimetry. The radiolabeled with {sup 99m}Tc was carried out using a direct method that involves the reduction of the anion TcO{sub 4}{sup -} with stannous chloride, so that the dendrimer is capable of coordinating to the technetium forming a chelate compound. The radiochemical purity of the radiolabeled compound was determined by thin layer chromatography using a sodium chloride solution to 20% (m/v) as mobile phase and was verified by molecular exclusion chromatography. The radiolabeled compound was possible to obtain it with a radiochemical purity superior to 90%. Also, the specific and not specific union was evaluated of the synthesized compound in mouse pancreas cancer cells AR42J, positive to somatostatin receptors, showing specific recognition for this receptors type with high cellular internalization. The biodistribution studies were carried out in BALB/c mice at different post injection times and in nude mice with induced tumors AR42J. The results showed that the {sup 99m}Tc-PAMAM-Tyr{sup 3}-Octreotide is excreted by via renal as hepatobiliary with a tumor capture of 4.12±0.6 D.I.g{sup -1} at the two hours of having been administered, which shows their potential as new radiopharmaceutical similar of somatostatin for the tumors detection of neuroendocrine origin. (Author)

  13. Evidence about chlorophyllin can function as an inhibitor or promoter of induced genetic damage by gamma radiation in Drosophila somatic cells; Evidencia de que la clorofilina puede funcionar como un inhibidor o un promotor del dano genetico inducido por radiacion gamma en celulas somaticas de Drosophila

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, A.E.; Cruces, M.P.; Zimmering, S.I. [Departamento de Biologia, ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    The irradiation of the individuals that had been feed during 24 hours with a chlorophyllin solution at 5 % was delayed from 0 until 4 days. The protector effect of chlorophyllin persisted by 3 days and it appears to coincide with the stage when stopping the mitotic divisions in the imagal disk. Within of the same cellular population, it was demonstrated that chlorophyllin can function as an inhibitor, in the case of simple stains mwh or a potentiator in the case of twin stains and the flr type stains. It was planned an explanation for these results. (Author)

  14. Study and development of membrane electrode assemblies for Proton Exchange Membrane Fuel Cell (PEMFC) with palladium based catalysts; Estudo e desenvolvimento de conjuntos membrana-eletrodos (MEA) para celula a combustivel de eletrolito polimerico condutor de protons (PEMFC) com eletrocatalisadores a base de paladio

    Energy Technology Data Exchange (ETDEWEB)

    Bonifacio, Rafael Nogueira

    2013-07-01

    PEMFC systems are capable of generating electricity with high efficiency and low or no emissions, but durability and cost issues prevent its large commercialization. In this work MEA with palladium based catalysts were developed, Pd/C, Pt/C and alloys PdPt/C catalysts with different ratios between metals and carbon were synthesized and characterized. A study of the ratio between catalyst and Nafion Ionomer for formation of high performance triple-phase reaction was carried out, a mathematical model to implement this adjustment to catalysts with different relations between metal and support taking into account the volumetric aspects of the catalyst layer was developed and then a study of the catalyst layer thickness was performed. X-ray diffraction, Transmission and Scanning Electron Microscopy, X-ray Energy Dispersive, Gas Pycnometry, Mercury Intrusion Porosimetry, Gas adsorption according to the BET and BJH equations, and Thermo Gravimetric Analysis techniques were used for characterization and particle size, specific surface areas and lattice parameters determinations were also carried out. All catalysts were used on MEAs preparation and evaluated in 5 cm{sup 2} single cell from 25 to 100 °C at 1 atm and the best composition was also evaluated at 3 atm. In the study of metals for reactions, to reduce the platinum applied to the electrodes without performance losses, Pd/C and PdPt/C 1:1 were selected for anodes and cathodes, respectively. The developed MEA structure used 0,25 mgPt.cm{sup -2}, showing power densities up to 550 mW.cm{sup -2} and power of 2.2 kW{sub net} per gram of platinum. The estimated costs showed that there was a reduction of up to 64.5 %, compared to the MEA structures previously known. Depending on the temperature and operating pressure, values from US$ 1,475.30 to prepare MEAs for each installed kilowatt were obtained. Taking into account recent studies, it was concluded that the cost of the developed MEA is compatible with PEMFC stationary application. (author)

  15. Study of the Nafion quantity effect in membrane and electrodes assemblies (MEAs) of 50 cm{sup 2} used in type proton exchange membrane (PEM) fuel cell operating with H{sub 2}/Air; Estudo do efeito da quantidade de Nafion em MEAs de 50 cm{sup 2} utilizadas em celula a combustivel tipo PEM operando com H{sub 2}/ar

    Energy Technology Data Exchange (ETDEWEB)

    Profeti, Demetrius; Colmati, Flavio; Carlindo, Adao A.J.; Paganin, Valdecir A.; Gonzalez, Ernesto R.; Ticianelli, Edson A. [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Inst. de Quimica]. E-mail: dprofeti@iqsc.usp.br

    2008-07-01

    The performance of a proton exchange membrane fuel cell (PEMFC) was investigated with the aim at characterizing the effects of the Nafion. content on the scale-up of the electrodes from 5 to 50 cm{sup 2}. It is observed that a diminution of the single cell performance occurred when the electrode area is increased from 5 to 50 cm{sup 2}. The tests carried out with different Nafion. contents, and fuel cell and humidifiers at the same temperature (T{sub cell}=T{sub H2}=T{sub air}=70 deg C) showed a slightly decrease of the fuel cell performance compared to the tests performed at different temperatures (T{sub cell}=70 deg C, T{sub H2}=85 deg C, T{sub air}=75 deg C). In the study of the variation on the Nafion. contents, the higher performance up to a current density of 0.8 A cm-2 is obtained with the 35.5 wt.% Nafion.. On the other hand, at higher current densities values, the performance of the fuel cells is very similar for the 31.0, 35.5 and 39.4 wt.% Nafion contents. (author)

  16. Preliminary analysis for implementation of a hybrid solar-wind system with storage of electrical energy generated through electrolytic hydrogen and fuel cells; Analise preliminar para implementacao de um sistema hibrido solar-eolico com armazenamento da energia eletrica gerada atraves de hidrogenio eletrolitico e celula a combustivel

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Daniel Gabriel; Furlan, Andre Luis; Lopes, Davi Gabriel [Universidade Estadual de Campinas (FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica], e-mail: danielg@fem.unicamp.br; Silva, Ennio Peres; Apolinario, Fernando Resende [Universidade Estadual de Campinas (IFGW/UNICAMP), SP (Brazil). Inst. de Fisica Gleb Wataghin. Lab. de Hidrogenio; Silva, Maria Eugenia Vieira da; Rocha, Paulo Alexandre Costa [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Engenharia Mecanica e de Producao; Codeceira Neto, Alcides [Companhia Hidro Eletrica do Sao Francisco (CHESF), Recife, PE (Brazil)

    2008-07-01

    Due to relevance of the study and applicability of hybrid electric power generation from solar photovoltaic and wind power in Brazil, the present paper aims to introduce briefly the importance of technical and economic comparison of two electrical energy storage technologies (batteries versus H2 + fuel cell) and also to indicate the previous difficulties related to this possible application. In this context, it was intended a partnership between State University of Campinas - UNICAMP, the Federal University of Ceara - UFC and Hydro Electric Company of the Sao Francisco - CHESF in the meaning of makes viable an implementation. (author)

  17. Exploratory study on the integration of fuel cell systems inside the chloro-soda plants for utilization of industrial hydrogen; Estudo exploratorio da integracao de sistemas de celula a combustivel dentro das plantas de cloro-soda para utilizacao do hidrogenio industrial

    Energy Technology Data Exchange (ETDEWEB)

    Braga, Jose Mauro Fernandes [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Escola de Engenharia]. E-mail: jmfbraga@oi.com.br; Seidl, Peter [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil); Pirro e Longo, Waldimir [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Nwcleo de Estudos Estrategicos (NEST)

    2008-07-01

    This work aims to demonstrate the economic viability of the application of industrial hydrogen in fuel cell systems by analyzing the advantages and disadvantages of this investment in hydrochloric acid/ sodium hydroxide plants. Using the Present Liquid Value and the Real Options Theory an economic evaluation will be made of the integration of fuel cell system in these plants, based on investment cost, volatility, among other parameters. (author)

  18. Comparative analysis between two systems to generate electric energy for isolated community in the interior of the Amazon state: fuel cells with natural gas reformer versus diesel generation; Analise comparativa entre dois sistemas de geracao de energia eletrica para a comunidade isolada no interior do estado do Amazonas: celula a combustivel com reformador para gas natural versus gerador diesel

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Paula Duarte; Bergamini, Cristiane Peres; Camargo, Joao Carlos; Lopes, Daniel Gabriel [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Fac. de Engenharia Mecanica; Esteves, Gheisa Roberta Telles [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Nucleo de Pesquisas e Estudos Ambientais; Silva, Ennio Peres da Silva [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Fisica Gleb Wataghin

    2004-07-01

    Although great part of the domestic territory is already supplied with electric energy, still there are many regions where the system is precarious or nonexistent, generically called isolated communities. In the majority of the cases these communities are supplied with Diesel oil generators and the substitution of this fuel for available alternative energy in the localities has been object of study of some institutions of research spread throughout the country. Currently, the use of fuel cells has been strongly argued in the generation of electric energy associated with the local energy necessity, from the use of a regional fuel and this is due to the high efficiency of allied energy conversion to the low ambient impacts that this equipment offers. Most of the different types of fuel cells use hydrogen as a fuel to produce electricity, and it is extracted from renewable or non-renewable sources of energy. Then, the article has the objective of comparing in first analysis the energy efficiency and the cost between the two systems: the ones used currently in the great majority of the isolated communities, constituted of a Diesel engine-generator system, with Natural Gas Reformer System/ Purifier of Hydrogen/ Fuel Cell/ and to analyze if such project presents characteristics that qualifies it to get the carbon credits proposed in the Mechanism of Clean Development. (author)

  19. Evaluation and application of PEMFC fuel cell's technologies developed at IPEN applied to a 500 W{sub e} fuel cell stack; Avaliacao e aplicacao de tecnologias de celulas a combustivel tipo PEMFC desenvolvida no IPEN em um modulo de 500 W{sub e} de potencia nominal

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, Edgar Ferrari da

    2009-07-01

    This work is part of a research project on PEMFC technologies carried out in IPEN to develop and optimize a 500 W{sub e} fuel cell stack. The MEAs scaling up from 25 cm{sup 2} to 144 cm{sup 2} produced by the method of sieve printing; computational fluid dynamics by computer simulation of gas flow channels in bipolar plates using COMSOL{sup R} program and the use of Pt/C electrodes developed by alcohol reduction method in single cells were used to build a stack of 500 W{sub e} nominal power for possible commercial applications, produced with national technology and industrial support. A 100 hours fuel cell's test was carried out in a 144 cm{sup 2} single cell to study the stability of the MEA fabricated by sieve printing method. This single cell showed good stability within this period of time. The developed stack has reached the maximum power of 574 W{sub e} at 100 A (694.4 mA cm{sup -2}). The operating power of 500 W{sub e} was obtained at 77.7 A (540.1 mA cm{sup -2}) and potential of 6.43 V, with efficiency of 43.3%. In terms of cogeneration, the thermal power or generated heat by the stack was 652 W{sub t}. The initial estimated cost for the 500 W{sub e} stack was about R$ 4,500.00, considering only the used materials for its construction. (author)

  20. Preparation and characterization of Nafion - TiO{sub 2} composite electrolytes for application in proton exchange membrane fuel cells; Preparacao e caracterizacao de eletrolitos compositos Nafion - TiO{sub 2} para aplicacao em celulas a combustivel de membrana de troca protonica

    Energy Technology Data Exchange (ETDEWEB)

    Matos, Bruno Ribeiro de

    2008-11-06

    The fabrication and characterization of Nafion - TiO{sub 2} composites, and the use of such electrolytes in PEM (Proton Exchange Membrane) fuel cell operating at high temperature (130 deg C) were studied. The operation of a PEM fuel cell at such high temperature is considered as an effective way to promote fast electrode reaction kinetics, high diffusional transport, and high tolerance to the carbon monoxide fuel contaminant. The polymer Nafion{sup R} is the most used electrolyte in PEM fuel cells due to its high proton conductivity. However, the proton transport in Nafion is dependent on the water content in the polymeric membrane. The need of absorbed water in the polymer structure limits the operation of the fuel cell to temperatures close to 100 deg C, above which Nafion exhibits a fast decrease of the ionic conductivity. In order to increase the performance of the electrolyte operating at high temperatures, Nafion-TiO{sub 2} composites have been prepared by casting. The addition of titania hygroscopic particles to the polymeric matrix aims at the enhancement of the humidification of the electrolyte at temperatures above 100 deg C. Three types of titania particles with different specific surface area and morphology have been investigated. Nafion-based composites with the addition of titania nanoparticles, in the 2.5-15 wt.% range, with nearly spherical shape and specific surface area up to approx. 115 m{sup 2}g{sup -1} were found to have higher glass transition temperature than the polymer. Such an increase improves the stability of the electrolyte during the fuel cell operation at high temperatures. The addition of titania-derived nanotubes results in a pronounced increase of the performance of PEM fuel cell operating at 130 deg C. In this composite, the high specific surface area and the tubular shape of the inorganic phase are responsible for the measured increase of both the absorption and retention of water of the composite electrolyte. Nonetheless, the polarization curves of fuel cell using the composite electrolytes exhibited an increase of the ohmic polarization associated with the addition of the insulating titania particles. As the chemical structure of Nafion was observed to be insensitive to the addition of the inorganic particles, the high performance of the composite electrolytes is a result of competing effects: the decrease of the electrical conductivity and a higher thermal stability or water absorption/retention capacity. The experimental results suggest that the Nafion-TiO{sub 2} composites are promising electrolytes for PEM fuel cells operating at temperatures above approx. 100 deg C. (author)

  1. Application of PtSn/C catalysts and Nafion SiO{sub 2} membranes in direct ethanol fuel cell at high temperatures; Aplicacao de catalisadores PtSn/C e membranas Nafion SiO{sub 2} em celulas a combustivel de etanol direto em elevadas temperaturas

    Energy Technology Data Exchange (ETDEWEB)

    Dresch, Mauro Andre

    2014-07-01

    This work has as objective to evaluate anodes and electrolytes in direct ethanol fuel cells (DEFC) operating at high temperature (130 deg C). As anode materials, electrocatalysts based on Pt Sn/C were prepared by Modified Polyol Method with various Pt:Sn atomic ratios. Such methodology promotes self organized electrocatalysts production with narrow particle size distribution and high alloying degree. The electrocatalysts were characterized by XRD, and CO stripping. The results showed that these materials presented high alloying degree and Eonset CO oxidation at lower potential as commercial materials. As electrolyte, Nafion-SiO{sub 2} hybrids were synthesized by sol-gel reaction, by the incorporation of oxide directly into the ionic aggregates of various kinds of Nafion membranes. The synthesis parameter, such sol-gel solvent, membrane thickness and silicon precursor concentration were studied in terms of silica incorporation degree and hybrid mechanical stability. Finally, the optimized anodes and electrolytes were evaluated in DEFC operating at 80 - 130 deg C temperature range. The results showed a significant improvement of the DEFC performance (122 mW cm{sup -2}), resulted from the acceleration of ethanol oxidation reaction rate due to anode material optimization and high temperature operation once the use of hybrids possibilities the increase of temperature without a significant conductivity loses. In this sense, the combination of optimized electrodes and electrolytes are a promising alternative for the development of these devices. (author)

  2. Evaluation of umbilical cord mesenchymal stem cells labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-Lysine; Avaliacao da marcacao de celulas-tronco mesenquimais de cordao umbilical com nanoparticulas superparamagneticas de oxido de ferro recobertas com Dextran e complexadas a Poli-L-Lisina

    Energy Technology Data Exchange (ETDEWEB)

    Sibov, Tatiana Tais; Mamani, Javier Bustamante; Pavon, Lorena Favaro; Cardenas, Walter Humberto; Gamarra, Lionel Fernel, E-mail: tatianats@einstein.br [Instituto do Cerebro - InCe, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Miyaki, Liza Aya Mabuchi [Faculdade de Enfermagem, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Marti, Luciana Cavalheiro; Sardinha, Luiz Roberto [Centro de Pesquisa Experimental, Hospital Israelita Albert Einstein - HIAE, Sao Paulo, SP (Brazil); Oliveira, Daniela Mara de [Universidade de Brasilia - UnB, Brasilia, DF (Brazil)

    2012-04-15

    Objective: The objective of this study was to evaluate the effect of the labeling of umbilical cord vein derived mesenchymal stem cells with superparamagnetic iron oxide nanoparticles coated with dextran and complexed to a non-viral transfector agent transfector poly-L-lysine. Methods: The labeling of mesenchymal stem cells was performed using the superparamagnetic iron oxide nanoparticles/dextran complexed and not complexed to poly-L-lysine. Superparamagnetic iron oxide nanoparticles/dextran was incubated with poly-L-lysine in an ultrasonic sonicator at 37 deg C for 10 minutes for complex formation superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine by electrostatic interaction. Then, the mesenchymal stem cells were incubated overnight with the complex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine and superparamagnetic iron oxide nanoparticles/dextran. After the incubation period the mesenchymal stem cells were evaluated by internalization of the complex superparamagnetic iron oxide nanoparticles/dextran/polyL-lysine and superparamagnetic iron oxide nanoparticles/dextran by Prussian Blue stain. Cellular viability of labeled mesenchymal stem cells was evaluated by cellular proliferation assay using 5,6-carboxyfluorescein-succinimidyl ester method and apoptosis detection by Annexin V- Propidium Iodide assay. Results: mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles/ dextran without poly-L-lysine not internalized efficiently the superparamagnetic iron oxide nanoparticles due to its low presence detected within cells. Mesenchymal stem cells labeled with the complex superparamagnetic iron oxide nanoparticles/dextran/polyL-lysine efficiently internalized the superparamagnetic iron oxide nanoparticles due to greater presence in the cells interior. The viability and apoptosis assays demonstrated that the mesenchymal stem cells labeled and not labeled respectively with the superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine continue to proliferate over seven days and the percentage of cells in early or late apoptosis is low compared to the percentage of live cells over the three days. Conclusion: Our results showed that the use of poly-L-lysine complexed with superparamagnetic iron oxide nanoparticles/dextran provides better internalization of these superparamagnetic iron oxide nanoparticles in mesenchymal stem cells Thus, we demonstrated that this type of labeling is not cytotoxic to the mesenchymal stem cells, since the viability and apoptosis assays showed that the cells remain alive and proliferating. The efficiency of this type of labeling in mesenchymal stem cells can provide non-invasive methods for monitoring these cells in vivo. (author)

  3. Reduction of greenhouse gases emissions listed in the Kyoto Protocol by the utilization of landfill gas using solid oxide fuel cells; Reducao das emissoes de gases de efeito estufa listados no protocolo de Quioto pelo aproveitamento do gas gerado em aterros sanitarios utilizando celulas a combustivel de oxido solido. Estudo de caso do aterro municipal de Santo Andre, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Paris, Alexandre Gellert

    2007-07-01

    In the last few years, the Kyoto Protocol had been a subject very debated, at first, in a restricted niche, manly academics and professionals related to the area of climate changes. On 16th February 2005 the Kyoto Protocol entered into force and with this a lot of publicity all over the world, so today is common to hear about it at the mass communications media. The extension of the subject is broad, this work discuss the utilization of one the Kyoto's flexibility mechanisms, to contribute to financing the use of the landfill gas in the solid oxide fuel cells. Among the three mechanisms presented in the Kyoto Protocol, the clean development mechanism (CDM) in article 12, is the only one that can be implemented by non-Annex I countries, the case of Brazil. In other hand, the issue of solid waste in Brazil is critical. Even being illegal, most of the solid waste goes to uncontrolled areas in open air places 'lixoes', causing degradation of the environment and the communities around this areas, and also emission of green house gases (GHG), deregulating the global climate system. Decontaminate this areas and the construction of landfills to replace than, considering the landfill as a bioreactor, and the utilization of the biogas to generate power can improve nowadays picture that we are facing. The utilization of an innovative alternative technology as the solid oxide fuel cell (SOFC) instead the conventional technologies will be more efficient and environmentally better. Among other barriers the cost is pointed as the biggest. In this context, the SOFC is the most expensive fuel cell, so the utilization of CDM can contribute to finance the application of this technology. Scenarios were made of 250 kW, 500 kW, 1,000 kW, 5,000 kW and 10,000 kW of installed power using biogas from the Municipal Landfill of Santo Andre. The calculations of the emission factor were done ex ante and ex post according to ACM0002. Comparing the costs of the installed power studied and the calculation of the emission reduction, a potential income, the costs are superior, however, other incomes from the selling of energy and the disposal of solid waste most be taken into account in the process of decision of this type of projects. (author)

  4. Labeling of vasoactive intestinal peptide (VIP) and VIP 10-28 fragment with radioiodine by direct method. Comparative study of the kinetics biodistribution and affinity for neuroendocrine tumor cells; Marcacao do peptideo intestinal vasoativo (VIP) e do fragmento VIP10-28 com radioiodo por metodo direto. Estudo comparativo da cinetica de biodistribuicao e da afinidade por celulas de tumor neuroendocrino

    Energy Technology Data Exchange (ETDEWEB)

    Colturato, Maria Tereza

    2005-07-01

    In the progress of the Nuclear Medicine, many protein based radiopharmaceuticals have been developed in the last years using antibodies and, more recently, biologically active natural peptides or similar synthetic peptides. In the search for agents with specificity for the target tissue in tumors detection, it was verified that small sequences of amino acids may interact with selective sites, with homogenous distribution, fast accumulation in tissues and fast blood clearance when compared to the antibodies. Among the peptides used in the diagnosis of tumors, Vasoactive Intestinal Peptide (VIP) has been studied. VIP labeled with iodine-123 is applied in the images of intestinal adenocarcinoma and endocrine tumors. The molecule of VIP contains two tyrosine residues, in the positions 10 and 22 that are, theoretically, equally susceptible to radioiodination for direct method. The objective of this work was to produce VIP labeled with radioiodine (iodine-123), in order to introduce to the brazilian medical class this radiopharmaceutical of interest for the diagnosis and recurrence of tumors that express specific receptors. In an unpublished way, the work studied the labeling and the kinetic distribution of the VIP fragment (VIP 10-28) and verified its potential as radiopharmaceutical applied in the identification of tumors that express VIP receptors. After the choice of the appropriated technique for labeling VIP and VIP 10-28 with radioiodine, using Ceremonial T as oxidant agent and sodium metabisulfite as reducing agent, the quality control procedures were accomplished (electrophoresis and high performance liquid chromatography, HPLC) for radiochemical purity determination as well as the separation of the radiochemical species obtained. Labeling and quality control procedures applied were efficient and accurate. [{sup 131}I]VIP and [{sup 131}l]VIP 10-28 were obtained with high radiochemical purity (> 95%). The purification studies to remove free radioiodine in the labeling mixture (simple purification) and to produce the radiopharmaceutical with high specific activity (complex purification and HPLC), were both efficient in the separation of the species in the reaction mixtures, as demonstrated by quality control procedures. Biological distribution studies were accomplished by venous administration of the radiopharmaceuticals in laboratory animals: biodistribution study of [{sup 131}I]VIP and [{sup 131}I]VIP 10-28 in normal Swiss mice, [{sup 131}I]VIP not purified and purified in Swiss mice with tumor and [{sup 131}I]VIP and [{sup 131}I]VIP 10-28 in Nude mice with tumor, scintigraphic images of [{sup 131/123}I]VIP and [{sup 123}I]VIP 10-28 in Swiss and Nude mice and Wistar rats with tumor. In vitro studies were accomplished to determine the percentage of [{sup 131}I]VIP and [{sup 131}I]VIP 10-28 bound to plasmatic proteins, stability study of [{sup 131}I]VIP and [{sup 131}I]VIP 10-28 in human plasma and the affinity and internalization of [{sup 131}I]VIP and [{sup 131}I]VIP 10-28 by tumour adenocarcinoma cells of human rectal colon (HT-29). All biological distribution studies demonstrated that both [{sup 131/123}I]VIP and [{sup 131/123}I]VIP 10-28 showed fast blood clearance, low renal and liver uptake, relative uptake in thyroid, showing in vivo dehalogenation and good uptake in tumour. Comparative biological distribution of the radiopharmaceuticals showed high uptake in the stomach for both peptides. The blood clearance of the fragment was slower, and influences the visualization of the tumour mass. The radiopharmaceuticals, [{sup 123}I]VIP and [{sup 123}I]VIP 10-28, were obtained with high radiochemical purity, but with low radiochemical yield when comparing with labeling procedures using iodine-131. Quality control assays of [{sup 123}I]Na indicated that the presence of radiochemical and radionuclide impurities influenced on labeling results. (author)

  5. Analysis for impedance electrochemistry 'on-line' of membrane/electrode assemble (MEA) of protons exchange membrane fuel cells (PEMFC); Analise por impedancia eletroquimica 'on-line' de conjuntos eletrodos/membrana (MEA) de celulas a combustivel a membrana polimetrica (PEMFC)

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Antonio Rodolfo dos

    2007-07-01

    This work reports results of studies and characterization on membrane electrode assemblies (MEAs) for proton exchange membrane fuel cell (PEMFC). Some cell operation conditions and different processes of MEA production were investigated. The electrochemical impedance spectroscopy technique (EIS) (in situ - 0 to 16 A) was used 'on-line' as a tool for diagnosis, concerning the cell performance. The EIS measurements were carried out with a FC350 Fuel Cell EIS System (GAMRY), coupled to a PC4 potentiostat/galvanostat and connected to the electronic load (TDI) for 'on-line' EIS experiments (100 mHz - 10 kHz, dU = 5 mV). MEAs with 25 cm{sup 2} surface area, using PtM/C 20% (M Ru, Sn or Ni) electrocatalysts were manufactured using the alcohol reduction process (ARP). The catalytic ink was applied directly into the carbon cloth (GDL) and pressed in the Nafion membrane (105). MEAs using Pt/C and Pt Ru/C 20% from E-TEK electrocatalysts were manufactured by comparison. All the cathodes were sprayed with Pt/C 20% from E-TEK. The noble metal concentrations used were set to 0.4 mg Pt.cm{sup -2} at the anode and 0.6 mg Pt.cm{sup -2} at the cathode (E-TEK). Nyquist diagrams of the MEAs with Pt/C and PtRu/C from E-TEK or PtM/C (M = Ru, Sn or Ni) ARP showed essentially the same ohmic resistances for the MEAs. This fact can be explained by suppression of agglomerates during the MEA preparation process or by the homogeneity of the anchored electrocatalysts at the carbon surface. It could also be observed, at low current densities, that there was a significant performance difference between the electrocatalysts from E-TEK and those prepared with the alcohol reduction process. The polarization curves results confirmed that the Pt M/C (M = Ru, Sn or Ni) ARP showed an activity increase for the methanol and ethanol fed cells. The technique of EIE was shown efficient for the evaluation of the method preparation of MEAs and the acting of the cell, the results of EIE showed coherence in the choice of the model the electric circuit for MEAs using hydrogen, methanol and ethanol. This coherence indicates that other resistances no considered in the model are not relevant in the total resistance of MEAs. (author)

  6. Fluorescein isothiocyanate labeled, magnetic nanoparticles conjugated D-penicillamine-anti-metadherin and in vitro evaluation on breast cancer cells; Avaliacao do isotiocianato de fluoresceina marcado, das nanoparticulas magneticas conjugadas da D-penicilamina antimetaderina e in vitro nas celulas do cancer de mama

    Energy Technology Data Exchange (ETDEWEB)

    Akca, Ozlet; Unak, Perihan; Medine, E. Ylker [Ege University (Turkey). Institute of Nuclear Sciences. Department of Nuclear Applications; Sakarya, Serhan [Adnan Menderes University (Turkey). ADUBILTEM Science and Technology Research and Development Center; Ozdemir, Caglar; Timur, Suna [Ege University (Turkey). Science Faculty. Department of Nuclear Applications

    2011-07-01

    Silane modified magnetic nanoparticles were prepared after capped with silica generated from the hydrolyzation of tetraethyl orthosilicate (TEOS). Amino silane (SG-Si900) was added to this solution for surface modification of silica coated magnetic particles. Finally, D-penicillamine (D-PA)-antimetadherin (anti-MTDH) was covalently linked to the amine group using glutaraldehyde as cross-linker. Magnetic nanoparticles were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and atomic force microscopy (AFM). AFM results showed that particles are nearly monodisperse, and the average size of particles was 40 to 50 nm. An amino acid derivative D-PA was conjugated anti-MTDH, which results the increase of uptaking potential of a conjugated agent, labelled fluorescein isothiocyanate (FITC) and then conjugated to the magnetic nanoparticles. In vitro evaluation of the conjugated D-PA-anti-MTDH-FITC to magnetic nanoparticle was studied on MCF-7 breast cancer cell lines. Fluorescence microscopy images of cells after incubation of the sample were obtained to monitor the interaction of the sample with the cancerous cells. Incorporation on cells of FITC labeled and magnetic nanoparticles conjugated D-PA-anti-MTDH was found higher than FITC labeled D-PA-anti-MTDH. The results show that magnetic properties and application of magnetic field increased incorporation rates. The obtained D-PA-anti-MTDH-magnetic nanoparticles-FITC complex has been used for in vitro imaging of breast cancer cells. FITC labeled and magnetic nanoparticles conjugated D-PA-anti-MTDH may be useful as a new class of scintigraphic agents. Results of this study are sufficiently encouraging to bring about further evaluation of this and related compounds for ultraviolet magnetic resonance (UV-MR) dual imaging. (author)

  7. Induced thermal ablation with a radiofrequency field in breast cancer cells using gold nanoparticles conjugated to the peptide cycle[RGDfK(C)]; Termoablacion inducida con un campo de radiofrecuencia en celulas de cancer de mama utilizando nanoparticulas de oro conjugadas al peptido ciclo[RGDfK(C)

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez H, L.

    2014-07-01

    The conjugation of peptides to gold nanoparticles (AuNP) produces biocompatible and stable multimeric systems with target-specific molecular recognition. Peptides based on the cyclic Arg-Gly-Asp (RGD) sequence have been reported as high-affinity agents for the α(v)β(3) and α(v)β(v) integrin s over expressed in breast cancer cells. AuNP have also been proposed as localized heat sources for cancer treatment using laser irradiation or radiofrequency (RF). The objective of this research was to evaluate the thermo ablative effect of the AuNP-c [RGDfK(C)] system on MCF7 breast cancer cell viability after exposure to a radiofrequency field and to compare it with that produced by the laser irradiation. The effect of the 13.56 MHz RF (using a power from 0 to 200 W at intervals of 50 W) over the temperature increase in AuNP-colloidal system of 5 and 20 nm at two different concentrations was evaluated. The absorption cross sections (C{sub abs}) of the AuNP-c [RGDfK(C)] nano system when it interacts with low frequency electromagnetic waves (13.56 MHz, λ = 22 m) and optical frequency waves (laser at λ = 532 nm) was analyzed based on the Mi e theory. The effect on the MCF7 cell viability was assessed using two thermal conversion sources (Laser and RF) on AuNP-c [RGDfK(C)] located inside the cytoplasm of the cells. MCF7 cells were treated with AuNP-c [RGDfK(C)] or water after exposure to the RF field (200 W, 100 V/cm) or laser irradiation (Irradiance 0.65 W/cm{sup 2}). In both cases (RF and laser) the presence of nanoparticles internalized inside the cells caused a significant increase in the temperature of the medium (RF: ΔT = 29.9 ± 1.7 grades C for AuNP compared toΔT = 13.0 ± 1.4 grades C for water; laser: ΔT = 13.5 ± 0.7 grades C for AuNP compared to 3.3 ± 0.5 grades C for water). Although RF induced a higher increase in the temperature of the medium with nanoparticles, the largest effect on the cell viability was produced by laser when nanoparticles were located inside the cells (8.7 ± 0.7 % for laser compared to 19.4 ± 0.9 % for RF). The differences obtained in C{sub abs} values (laser: 3.7x10{sup -16} m{sup 2}; RF: 7.9x10{sup -23} m{sup 2}) and the observed effect on MCF7 cell viability support two mechanisms proposed -optical frequency wave energy absorption by AuNP- when laser is used as a thermal conversion source, and -energy attenuation of the low frequency electromagnetic wave propagating through the AuNP-colloidal system- when RF field is applied. The AuNP-c[RGDfK(C)] nano system shows suitable properties to improve hyperthermia treatments under laser irradiation due to a larger heat release inside cells. (Author)

  8. Studies on the efficiency during reactivation of a generation system based on natural gas reformer and a 5 k W fuel cell; Estudos de eficiencia durante reativacao de um sistema de geracao baseado em reformador de gas natural e celula a combustivel de 5 kW

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Francisco da Costa; Furtado, Jose Geraldo de Melo; Silva Junior, Fernando Rodrigues da; Serra, Eduardo Torres [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)]. E-mail: fcl@cepel.br

    2008-07-01

    Fuel cell based power generation systems have been pointing as promising technology for stationary applications mainly to supply power to critical loads. Among several types of fuel cells the Polymer Electrolyte Membrane Fuel Cells (PEMFC) are the main type used around the world. Nowadays reformers are widely employed to produce hydrogen for fuel cells. The Fuel Cell Laboratory of CEPEL has a power plant based on a 5 kW PEMFC and a natural gas reformer. For a long time the PEMFC was inoperable due to reformer malfunctioning and during this time the full power availability of PEMFC was lost due to deactivation of its catalytic sites. In most cases this deactivation is reversible. So it was started a reactivation process aiming to recover the full operational condition of the PEMFC unit. During this process the gas flow relationship and efficiency of the reformer were studied. An analysis of the PEMFC reactivation was conducted where it was noted that the reactivation took place as expected. During the reactivation process the PEMFC and the whole system efficiency were analyzed. The results suggest that the PEMFC can reach efficiency compatible with conventional power generation systems thus allowing PEMFC technology to compete with these energy sources in point of efficiency. (author)

  9. Development of a membrane electrode as assembly production process for proton exchange membrane fuel cell (PEMFC) by sieve printing; Desenvolvimento de processo de producao de conjuntos eletrodo-membrana-eletrodo para celulas a combustivel baseadas no uso de membrana polimerica conditora de protons (PEMFC) por impressa a tela

    Energy Technology Data Exchange (ETDEWEB)

    Bonifacio, Rafael Nogueira

    2010-07-01

    Energy is a resource that presents historical trend of growth in demand. Projections indicate that future energy needs will require a massive use of hydrogen as fuel. The use of systems based on the use of proton exchange membrane fuel cell (PEMFC) has features that allow its application for stationary applications, automotive and portable power generation. The use of hydrogen as fuel for PEMFC has the advantage low pollutants' emission, when compared to fossil fuels. For the reactions in a PEMFC is necessary to build membrane electrode assembly (MEA). And the production of MEAs and its materials are relevant to the final cost of k W of power generated by systems of fuel cell. This represent currently a technological and financial barriers to large-scale application of this technology. In this work a process of MEAs fabrication were developed that showed high reproducibility, rapidity and low cost by sieve printing. The process of sieve printing and the ink composition as a precursor to the catalyst layer were developed, which allow the preparation of electrodes for MEAs fabrication with the implementation of the exact catalyst loading, 0.6 milligrams of platinum per square centimeters (mgPt.cm{sup -2}) suitable for cathodes and 0.4 mgPt.cm{sup -2} for anode in only one application step per electrode. The ink was developed, produced, characterized and used with similar characteristics to ink of sieve printing build for other applications. The MEAs produced had a performance of up to 712 m A.cm{sup -2} by 600 mV to 25 cm{sup 2} MEA area. The MEA cost production for MEAs of 247.86 cm{sup 2}, that can generate 1 kilowatt of energy was estimated to US$ 7,744.14 including cost of equipment, materials and labor. (author)

  10. Cultivation and irradiation of human fibroblasts in a medium enriched with platelet lysate for obtaining feeder layer in epidermal cell culture; Cultivo e irradiacao de fibroblastos humanos em meio enriquecido com lisado de plaquetas para obtencao de camada de sustentacao em culturas de celulas da epiderme

    Energy Technology Data Exchange (ETDEWEB)

    Yoshito, Daniele

    2011-07-01

    For over 30 years, the use of culture medium, enriched with bovine serum, and murines fibroblasts, with the rate of proliferation controlled by irradiation or by share anticarcinogenic drugs, has been playing successfully its role in assisting in the development of keratinocytes in culture, for clinical purposes. However, currently there is a growing concern about the possibility of transmitting prions and animals viruses to transplanted patients. Taking into account this concern, the present work aims to cultivate human fibroblasts in a medium enriched with human platelets lysate and determine the irradiation dose of these cells, for obtaining feeder layer in epidermal cell culture. For carrying out the proposed objective, platelets lysis has standardized, this lysate was used for human fibroblasts cultivation and the irradiation dose enough to inhibit its duplication was evaluated. Human keratinocytes were cultivated in these feeder layers, in culture medium enriched with the lysate. With these results we conclude that the 10% platelets lysate promoted a better adhesion and proliferation of human fibroblasts and in all dose levels tested (60 to 300 Gy), these had their mitotic activity inactivated by ionizing irradiation, being that the feeder layers obtained with doses from 70 to 150 Gy were those that provided the best development of keratinocytes in medium containing 2.5% of human platelet lysate. Therefore, it was possible to standardize both the cultivation of human fibroblasts as its inactivation for use as feeder layer in culture of keratinocytes, so as to eliminate xenobiotics components. (author)

  11. The importance of investigation of anatomic variations in the ethmoid cells by computerized tomography in the planning of endoscopic nasal surgery; A importancia da investigacao de variacoes anatomicas das celulas etmoidais atraves da tomografia computadorizada no planejamento da cirurgia endoscopica nasal

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz, Cristiane Regina; Wafae, Nader; Nascimento, Sergio Ricardo Rios; Camelo, Julio Cesar; Maciel, Luana Teixeira; Consolari, Mario Agostinho Severo [Centro Universitario Sao Camilo, Sao Paulo, SP (Brazil)], e-mail: crisruiz@saocamilo-sp.br, e-mail: riosnascimento@uol.com.br

    2009-01-15

    The ethmoidal cells comprise many cavities that show many anatomical variations. Some of these variations deserve special attention when associated with sinusitis and the need of surgery. Computed tomography, considered a gold standard on the pathological investigation of the nasal cavity and the anatomic description of paranasal sinus, offers information about these cells, essential to minimize the surgical risks. The authors present a bibliographic revision of the importance of computed tomography on the investigation of anatomic variations of ethmoid cells. Not recurrent cells in all individuals, like Haller's cells, Onodi's cells and pneumatization of agger nasi, will do the endoscopic approach with precaution. The ethmoidal roof evaluation will result in more safety on the upper limits of the nasal cavity. The size and pneumatization level of ethmoidal bulla, ethmoidal crest, and middle nasal concha are important on the evaluation of the endoscopic accesses and the gravity of the stroked pathology and even diagnose your origin. (author)

  12. Dramaturgia Híbrida : o corpo que dança e o trabalho com as ações físicas a partir de experiências no Teatro Varasanta = Hybrid Dramaturgy: the boding dance and the work with the physical actions from experiences in Teatro Varasanta

    OpenAIRE

    Daniela Rolim Machado Moreno Zuliani

    2015-01-01

    Tenho como proposta pesquisar processos de hibridação de práticas e linguagens da cena que potencializam corpos criativos nas artes cênicas. Parto do estudo de dois elementos constitutivos da produção cênica contemporânea: o conceito de movimento dançado e o trabalho com as ações físicas, recorrente na prática teatral. Para o acontecimento de processos de hibridação entre esses dois fenômenos durante a criação, a compreensão do desenvolvimento de uma dramaturgia do corpo torna-se essencial pa...

  13. DESEMPEÑO DE LOS PROTOCOLOS DE ENRUTAMIENTO MESH

    Directory of Open Access Journals (Sweden)

    Danilo Alfonso López Sarmiento

    2014-05-01

    Full Text Available En este artículo se presentan los resultados obtenidos de la evaluación del desempeño de los principales protocolos que se podrían utilizar en enrutamiento malla, considerando tasas de entrega de paquetes, retardos y carga de enrutamiento promedio. La investigación estableció que el algoritmo de enrutamiento de fuente dinámico es el que posee mejor rendimiento debido a su naturaleza hibrida

  14. ENSO impacts on the South American rainfall during 1980s: Hadley and Walker circulation

    Energy Technology Data Exchange (ETDEWEB)

    De Souza, E.B.; Ambrizzi, T. [Departamento de Ciencias Atmosfericas, Instituto Astronomico y Geofisico, Sao Paulo, SP (Brazil)

    2002-04-01

    The changes in the Hadley and Walter cells and their respective impacts on the South American rainfall during the ENSO episodes observed in the decade of 80, were investigated through cross-sections analyses of the atmospheric circulation in altitude, averaged in the zonal and meridional planes. Such large-scale cells almost inverted their climatological circulation pattern, during El Nino events (1982-83 and 1986-87). In these years, manifestation of the anomalous descending branch of the Hadley and Walker cells affects most of the north-northeast of South America, which inhibited the convective activity associated to the Inter Tropical Convergence Zone (ITCZ) and caused drought conditions in the rainy seasons of the Guyana, Surinam, French Guyana, center-east of the Amazon and most of the Northeast Brazil. On the other hand, conditions of excess of precipitation observed in the south-southeast of South America, were favored by the anomalous ascending branch of the Hadley cell. During La Nina events (1984-85 and 1988-89), it was observed an intensification of the ascending and descending branches associated to the Walker and Hadley cells. The anomalous large-scale ascending movement associated to these cells, was extended to the Northeast of Brazil and equatorial South Atlantic, favoring ITCZ to become more active than the normal, which resulted in an above normal rainy season in these areas. An intense subsidence was noticed in the mid latitudes of South America, which inhibited the large-scale convection in the region, explaining the deficient rainy season observed in most of the south-southeast of South America. [Spanish] Los cambios en las celulas de Hadley y Walker y sus respectivos impactos en Suramerica, en las lluvias, durante los episodios en ENOS, observados en la decada de los 80, son investigados a traves de analisis de reacciones cruzadas de la circulacion atmosferica en altitudes promediadas en planos zonal y meridional. Tales celulas de gran escala

  15. Central effects of fingolimod.

    Science.gov (United States)

    Cruz, Vítor T; Fonseca, Joaquim

    2014-08-01

    Introduccion. El fingolimod, un modulador del receptor de la esfingosina-1-fosfato (S1P) dotado de un mecanismo de accion novedoso, fue el primer tratamiento oral aprobado para la esclerosis multiple remitente recurrente. Su union a los receptores S1P1 de los linfocitos promueve la retencion selectiva de los linfocitos T virgenes y de memoria central en los tejidos linfoides secundarios, lo que impide su salida hacia el sistema nervioso central (SNC). Asimismo, el fingolimod atraviesa con facilidad la barrera hematoencefalica, y diversos estudios le atribuyen un efecto neuroprotector directo en el SNC. Objetivo. Revisar la informacion disponible acerca de los efectos centrales del fingolimod. Desarrollo. El desequilibrio entre los procesos lesivos y reparadores constituye un reflejo de la desmielinizacion cronica, la degeneracion axonal y la gliosis, y parece contribuir a la discapacidad que la esclerosis multiple acarrea. La facilidad con la que el fingolimod atraviesa la barrera hematoencefalica le permite actuar directamente sobre los receptores S1P localizados en las celulas del SNC. Una vez en el interior del SNC, ocupa los receptores S1P de los oligodendrocitos y de sus celulas precursoras, de los astrocitos, los microgliocitos y las neuronas, fomentando la remielinizacion, la neuroproteccion y los procesos endogenos de regeneracion. La eficacia evidenciada en los ensayos clinicos concuerda con un mecanismo de accion que incluiria efectos directos sobre las celulas del SNC. Conclusiones. Los datos disponibles indican que la eficacia del fingolimod en el tratamiento de la esclerosis multiple se debe a su ambivalencia como molecula inmunomoduladora y moduladora directa de los receptores S1P del SNC. Tanto es asi que estudios recientes le atribuyen efectos neuroprotectores en varios modelos que suscitan expectativas en torno a su posible aplicacion terapeutica en la enfermedad de Alzheimer, el paludismo cerebral y el neuroblastoma, asi como en la neuroproteccion

  16. Study on In-Theater Maintenance Support for POAF F-16

    Science.gov (United States)

    1991-09-01

    0.9 0.4 0.9 1.1 30 0.4 0.4 0.9 1.2 62 z A CO) 00 b, w - Q w V,> CI + Q ) CM 4bS *b 63q AVERAGE DAILY SORTIES FLOWN SCENARIO DAY M N 0 P 1 10.3 10.2 10.1...1990. Permanent Address: Celula C - Lote 58A - 6 C Quinta Grande Alfragide 2700 Amadora, Portugal 79 I Form ApprOvedJ REPORT DOCUMENTATION PAGE om Novd4

  17. Isolating of Target Genes for NKX3.1 in Prostate Carcinogenesis

    Science.gov (United States)

    2005-03-01

    stainin on e te o tesr prostate hist log .(Band - . (C a .. ( 2 (M -P) Ki6 s shows . celula pr lf rto in c( ssi Fg1.prosthate cane r progresin is enh...from PIN or cancer lesions compoundp27kiPz heterozygotes (Figs. 1 Q -Tand 7). Therefore, the of these mice followed by real-time RT-PCR. We found that...9. Wang, S., Gao, J., Lei, Q ., Rozengurt, N., Pritchard, C., Jiao, J., Thomas, G. V., Li, G., 24. Kim, M. J., Bhatia-Gaur, R., Banach-Petrosky, W. A

  18. European Science Notes Information Bulletin Reports on Current European/Middle Eastern Science,

    Science.gov (United States)

    1989-07-01

    are traveling in Europe may also be invited to submit reports. ) Q j) 5 ’ W9, A, AP AR!T C59 4BAS7ACI 21 ABSTRACT SECURITY CLASSIFICATION E QAS 3 N V...ionic strengths and are inter-tem eguatig vaiou celula fuctins.preted as equilibrium shifts between the two main con-tern regulating various cellular...matrices covered the range of purifcation steps for recombinant Q Sepharose HP TM and Supcrdcx 75 pg were used. proteins from the primary separation to

  19. Estudo de 43 pacientes com atrofia muscular espinhal e seu diagnóstico molecular

    OpenAIRE

    Carvalho, Laís Regina Rocha de; Scola, Rosana Herminia

    2003-01-01

    Apendice e Anexos Orientadora: Rosana Herminia Scola Dissertaçao(mestrado)- Universidade Federal do Paraná. Setor de Ciencias da Saúde. Departamento de Clínica Médica. Curso de Pós-graduaçao em Medicina Interna Inclui bibliografia Resumo: A atrofia muscular espinhal e uma doenca degenerativa das celulas do como anterior da medula, com quatro tipos descritos (I, II, IIIa/b, IV). Somente os tipos I, II, Illa e Illb apresentam comprometimento do cromossomo 5ql3, em que dois genes sao...

  20. Cytogenetic and dosimetric effects of {sup 131}I in lymphocyte of patients with differentiated thyroid cancer with and without r-hTSH stimulation. Study in thyroid tumor cells (WRO) treated with {sup 131}I and {sup 60}Co in vitro; Efeitos citogenetico e dosimetrico do {sup 131}I em pacientes com cancer diferenciado da tireoide com e sem estimulacao com r-hTSH. Estudo em celulas tumorais tireoidianas (WRO) tratadas com {sup 131}I e {sup 60}Co in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Valgode, Flavia Gomes Silva

    2015-11-01

    Differentiated thyroid cancer (DTC) represents about 90% of thyroid malignancies with increasing incidence in the recent decades. Treatment modalities include thyroidectomy, {sup 131}I therapy (with or without r-hTSH), radio and chemotherapy. Little is known about the effects of these treatments at the cellular level. This work was proposed in order to assess to what extent radioiodine therapy can cause damage in peripheral lymphocytes of patients with DTC, preceded or not by r-hTSH, taking into account acute, slow and dosimetric effects of {sup 131}I (in vivo study). An in vitro study was also carried out on thyroid tumor target cells (WRO) by cytotoxicity and genotoxicity analysis and radioiodine uptake. For this, blood samples from patients divided into two groups (group A, r-hTSH + {sup 131}I and group B,{sup 131}I only) were collected before, 24 hours, 1 week, 1 month and 1 year after {sup 131}I administration for aberration chromosome analysis (CA). A dose-response curve for {sup 131}I in vitro was developed for estimating the absorbed dose in patients, comparing the dicentric frequencies obtained in vitro with in vivo data by Monte Carlo program. Radioiodine therapy induced an increase in the number of CA in lymphocytes of patients peaking 24 hours after treatment, with gradual decline over time and with more chromosomal damage in group B than in group A, reaching baseline levels one year after radioiodine administration. The frequency of dicentric found inpatient lymphocytes, 24h after treatment, was equivalent to that induced in vitro (0.354 ± 0.153 MBq / mL for group A and 0.309 ± 0.154 MBq / mL for group B), which corresponds to absorbed doses of 0.8 ± 0.3 Gy and 0.7 ± 0.3 Gy for groups A and B, respectively, with no significant difference between the groups. WRO cells showed a cell cycle relatively slow: 96,3h with an unstable karyotype. The genotoxic test showed a relatively high radioresistance (0.07 to 3.70 MBq/mL), with no statistical significance, with or without r-hTSH. However, the cytotoxic assay, showed a tendency to decrease at higher concentrations of 1.85 (p <0.05) and 3.70 MBq/ml (p <0.01) only in the presence of r-hTSH, coincident with the highest level of uptake. WRO cells were also relatively radioresistant to external irradiation of {sup 60}Co in the range of 0.2-8.3 Gy, with a gradual decrease in function of time for higher doses (10,20 and 40Gy).The data obtained showed little cytogenetic damage in patients upon therapeutic exposure, suggesting a safe and effective treatment for both groups of patients. Patients in group A, however, had a better quality of life by using r-hTSH. In vitro studies with internal ({sup 131}I) and external ({sup 60}Co) irradiation, with or without r-hTSH, point to the need for an alternative therapeutic strategy to overcome the loss of ability of thyroid cells (WRO) to concentrate radioiodine, which is responsible for the failure of radioiodine therapy in patients with DTC. (author)

  1. Mineral and/or milk supplementation of fruit beverages helps in the prevention of H2O2-induced oxidative stress in Caco-2 cells La adición de minerales y/o leche a bebidas a base de zumo de frutas ayuda en la prevención del estrés oxidativo inducido por H2O2 en celulas Caco-2

    Directory of Open Access Journals (Sweden)

    A. Cilla

    2011-06-01

    Full Text Available Introduction: Fruit beverages are commonly supplemented with milk, vitamins and/or minerals in order to improve their healthy effects by providing some bioactive components that can act additively or synergistically against oxidative stress. Aims: To test whether iron, zinc, and milk added to fruit beverages do not affect the cytoprotective effect against oxidative damage to Caco-2 cells through GSH-related enzymes induction and cell cycle progression preservation, in comparison with non-supplemented fruit beverage. Methods: Caco-2 cells were incubated 24 h with the bioaccessible fraction (BF of eight fruit beverages with/without iron and/or zinc, and/or milk, and then challenged with H2O2 (5 mmol L-1 -2 h. Mitochondrial enzyme activities (MTT test, GSH-Rd and GSH-Px enzyme activities, cell cycle progression and caspase-3 activity were measured. Results and discussion: Fruit beverages prevented the deleterious effect of H2O2 on cell viability, with almost all samples reaching control basal levels. Only independent iron or zinc supplementation with/without milk exerted positive effects upon GSH-Rd activity. Both minerals with milk, afforded improved preservation of GSH-Px activity. All samples prevented the decrease in the G1 phase of cell cycle induced by H2O2, except iron supplemented samples with/without milk, but none of them avoided the increase in sub-G1 phase. However, this fact was not associated to caspase-3 activity, with a probable positive effect of zinc upon this parameter. Conclusion: Mineral and/or milk supplementation of fruit beverages helps in the prevention of oxidative stress in Caco-2 cells based on cell viability maintenance, GSH-related enzymes activation, cell cycle distribution preservation and inhibition of caspase-3 activation.Introducción: En la actualidad las bebidas a base de zumo de frutas llevan adicionadas leche, vitaminas y/o minerales con objeto de mejorar sus efectos beneficiosos para la salud mediante el aporte de numerosos compuestos bioactivos que pueden actuar de forma aditiva o sinérgica frente al estrés oxidativo. Objetivos: Evaluar si la adición de hierro, cinc y leche a las bebidas a base de zumo de frutas no afecta al efecto cito-protector frente al daño oxidativo en células Caco-2 a través de la inducción de enzimas del ciclo del GSH y la preservación de la progresión del ciclo celular, en comparación con la bebida a base de zumo de frutas no suplementada. Métodos: Las células Caco-2 se incubaron 24 h con las fracciones bioaccesibles (FB de ocho bebidas a base de zumo de frutas con/sin hierro y/o cinc y/o leche, y se sometieron a estrés oxidativo con H2O2 (5 mmol L-1-2 h. Se determinó la actividad enzimática mitocondrial (test MTT, la actividad de las enzimas GSH-Rd y GSH-Px, la progresión del ciclo celular y la actividad de la enzima caspasa-3. Resultados y discusión: Las bebidas a base de zumo de frutas previnieron del efecto perjudicial del H2O2 sobre la viabilidad celular, con casi todas las muestras alcanzando los niveles basales del control. Sólo la adición independiente de hierro o cinc con/sin leche ejerció efectos positivos sobre la actividad de la enzima GSH-Rd. Por otra parte, ambos minerales con leche proporcionaron una mejor preservación en la actividad de la enzima GSH-Px. Todas las muestras previnieron el descenso en la fase G1 del ciclo celular inducido por el H2O2, excepto la muestra adicionada de hierro, pero ninguna de ellas evitó el incremento en la fase subG1 del ciclo celular. Sin embargo, este hecho no estuvo asociado con la actividad de la enzima caspasa-3, con un probable efecto positivo del cinc sobre este parámetro. Conclusión: La adición de minerales y/o leche a las bebidas a base de zumo de frutas ayuda en la prevención de estrés oxidativo en células Caco-2 mediante el mantenimiento de la viabilidad celular, activación de enzimas del ciclo del GSH, preservación de la progresión del ciclo celular e inhibición en la activación de la enzima caspasa-3.

  2. Synthesis and characterization of La{sub 1-x}Sr{sub x}MnO{sub 3} (x=0,1) for cathode application in solid oxide fuel cells (SOFC); Sintese e caracterizacao de La{sub 1-x}Sr{sub x}MnO{sub 3} (x=0,1) visando sua aplicacao como catodo em celulas a combustivel de oxido solido (SOFC)

    Energy Technology Data Exchange (ETDEWEB)

    Tarrago, D.P.; Sousa, V.C., E-mail: dptarrago@gmail.co [Universidade Federal do Rio Grande do Sul (LABIOMAT/PPGEM/UFRGS), Porto Alegre, RS (Brazil). Programa de Pos graduacao em Engenharia de Minas. Lab. de Biomateriais; Malfatti, C.F. [Universidade Federal do Rio Grande do Sul (LAPEC/PPGEM/UFRGS), Porto Alegre, RS (Brazil). Programa de Pos graduacao em Engenharia de Minas. Lab. de Pesquisa em Corrosao

    2010-07-01

    Perovskite powders, with composition La{sub 1-x}Sr{sub x}MnO{sub 3} (x=0,1) were obtained via combustion synthesis using sucrose as fuel. In the X-ray diffraction patterns it was observed that in order to obtain a single phase and well crystallized material a calcination in 750 deg C for 3 hours was necessary. BET analysis detected a specific surface area of 45m{sup 2}/g, considerably higher than when obtained with other fuels. SEM micrographs revealed a spongy aspect with a connected porosity in the agglomerates and though TEM micrographs the presence of pores in the particles was verified. The powder compacted with 125MPa and sintered at 1050 deg C for two hours presented a 31% open porosity and the SEM micrographs showed a fine interconnected porosity. (author)

  3. Evaluation of the radiosensitizing to treatment with {sup 153}Sm-EDTMP, of haematopoietic cells of the bone marrow by means of bromodeoxyuridine incorporation into DNA, in a murine model; Evaluacion de la radiosensibilizacion al tratamiento con {sup 153}Sm-EDTMP, de las celulas hemotopoyeticas de la medula osea mediante la incorporacion de bromodesoxiuridina (BrdU) en el ADN, en un modelo murino

    Energy Technology Data Exchange (ETDEWEB)

    Morales A, E.

    2008-07-01

    Bromodeoxyuridine (BrdU) has been shown to have a radiosensitizing effect, and its incorporation into DNA prior to administration of a bone-seeking radiopharmaceutical could increase the efficiency of bone marrow ablation, and even increase the specificity of radiation exposure for therapeutic purposes. The aim of the present study was to determine the effect of BrdU incorporation into DNA on the genotoxic and cytotoxic effects of samarium-153 ethylenediaminetetra-methylene phosphonate ({sup 153}Sm-EDTMP) in murine bone marrow cells. BALB/c male mice (N = 5 in each experiment) were treated with one of the following substances: a) BrdU (0.25 mg/g) b) {sup 153}-EDTMP (11.5 +- 3 MBq) c) BrdU (0.25 mg/g) plus {sup 153}Sm-EDTMP (11.5 +- MBq), there was also an untreated control. Cytotoxicity and genotoxicity were established by time-response and absorbed dose-response curves of polychromatic erythrocyte (PCE) and micro nucleated polychromatic erythrocyte (MN-PCE) frequencies, respectively, in murine peripheral blood samples in vivo. The significance of the differences between groups was determined by a variation of Dunett test for multiple groups and different-sized groups of a student test. Beta-absorbed dose fractions obtained from MNCP4B Monte Carlo computer code were used for mice bone marrow dosimetry calculations. At an average radiation absorbed dose of 0.38 Gy, 0.56 Gy and 0.82 Gy at 24, 40 and 72 h respectively, cells from animals treated with {sup 153}Sm-EDTMP showed a clear and significant induction of MN-PCE after 24 h, with the maximum response at 40 h, however, cells from group treated with BrdU plus {sup 153}Sm-EDTMP paradoxically showed MN-PCE frequencies only slightly higher than the control at the same absorbed dose. Treatment with {sup 153}Sm-EDTMP caused a slight reduction in PCE frequency, but exposure to BrdU or BrdU plus {sup 153}Sm-EDTMP induced a substantial and significant reduction in PCE frequency from 32 h to the end of the experiment (72 h). The PCE frequencies in the BrdU pretreated and irradiated group were consistently lower than in the BrdU control group, and the difference was significant at the final scored times in the experiment. These effects could be explained by lethal lesions of the accumulation of sublethal damage induced by the irradiation of BrdU-substituted DNA, where this lesion is not easily repaired and subsequently does not permit cell division or micronucleus formation. This research shows the increasing of cytotoxicity when BrdU was administered before the {sup 153}Sm-EDTMP treatment. Further studies are required in order to explore the utility of this strategy for bone marrow ablation or therapy in humans. (Author)

  4. Preparation of PtSn/C, PtRu/C, PtRh/C, PtRuRh/C and PtSnRh/C electrocatalysts using an alcohol-reduction process for methanol and ethanol oxidation; Preparacao e caracterizacao de eletrocatalisadores PtRu, PtSn, PtRh, PtRuRh e PtSnRh para oxidacao direta de alcoois em celulas a combustivel tipo PEM utilizando a metodologia da reducao por alcool

    Energy Technology Data Exchange (ETDEWEB)

    Dias, Ricardo Rodrigues

    2009-07-01

    In this work, Pt/C, PtRh (90:10), PtRh/C (50:50), PtSn/C (50:50), PtRu (50:50)/C, PtRuRh/C (50:40:10) and PtSnRh/C (50:40:10) were prepared by an alcohol-reduction process with metal loading of 20 wt.% using H{sub 2}PtCl{sub 6}.6H{sub 2}O (Aldrich), SnCl{sub 2}.2H{sub 2}O (Aldrich),and RhCl{sub 2}.XH{sub 2}O (Aldrich) as metals sources and Vulcan XC72 as support. The electrocatalysts were characterized by EDX, XRD and cyclic voltammetry (CV). The electro-oxidation of ethanol was studied by CV, chronoamperomety at room temperature in acid medium and tests at 100 deg C on a single cell of a direct methanol or ethanol fuel cell. The EDX analysis showed that the metal atomic ratios of the obtained electrocatalysts were similar to the nominal atomic ratios used in the preparation. The diffractograms of electrocatalysts prepared showed four peaks at approximately 2{theta} =40 deg, 47 deg, 67 deg and 82 deg, which are associated with the (111), (200), (220) and (311) planes, respectively, of a face cubic-centered (fcc) structure characteristic of platinum and platinum alloys. The average crystallite sizes using the Scherrer equation and the calculated values were in the range of 2-3 nm. For Pt Sn/C and PtSnRh/C two additional peaks were observed at 2 = 34 deg and 52 deg that were identified as a SnO{sub 2} phase. Pt Sn/C (50:50) and PtSnRh/C (50:40:10) electro catalyst showed the best performance for ethanol oxidation at room temperature. For methanol oxidation at room temperature Pt Ru/C, Pt Sn/C and PtRuRh/C electrocatalysts showed the best performance. Tests at 100 deg C on a single cell of a direct ethanol fuel cell PtSnRh/C showed the best performance, for methanol oxidation PtRuRh/C showed the best performance. (author)

  5. Estudos sobre tuberculose

    Directory of Open Access Journals (Sweden)

    A. Fontes

    1917-01-01

    Full Text Available Assim em resumo pensamos que a hipersensibilidade na infeção tuberculosa é a expressão duma reação celular, autonoma e individual, consequente á agressão brusca e á destruição (dijestão incompleta dos toxicos bacilares (proteinas, globulinas e lipoides, em combinação intima ou isoladamente; a adição aos toxicos bacilares de lipoides diversos (cholesterina, lecitina, esters e principios mal definidos existentes no oleo de figado de bacalhau, atenua a sua função hipersensibilidade porque torna esses toxicos mais facilmente incorporaveis á celula sensivel por adsorpção da substancia toxica ao lipoide. Esses corpos penetram assim no interior das celulas levando comsigo ativadores dos processos enzimaticos que não so favorecem uma elaboração dijestiva mais perfeita como ainda uma melhor produção de anticorpos. O aparecimento de pequenas modalidades reacionarias no decurso duma immunisação ativa contra os antijenos saturados por lipoides mostra a necessidade dum processo de desintegração para que a função toxica se manifeste.

  6. Estudo comparativo das inclusões do alastrim e da vacina no macaco (Macacus rhesus A comparison of the inclusion bodies of alastrim and vaccinia in the monkey (Macacus rhesus

    Directory of Open Access Journals (Sweden)

    C. Magarinos Torres

    1934-02-01

    Full Text Available Vesiculas e pustulas contendo numerosas inclusões citoplasmicas nas celulas epidermicas, foram regularmente produzidas no macaco (Macacus rhesus, quer com o virus do alastrim, quer com o da vacina, após inoculação endovenosa e sem previa escarificação. O virus do alastrim parece menos virulento para essa especie de macaco que o da vacina. Ao passo que 12 macacos rhesus injetados por via endovenosa com sete amostras diferentes de virus do alastrim, após apresentarem com regularidade um infecção experimental, sobreviveram e se conservaram em boa saúde, a injecção endovenosa do virus da vacina recentemente preparado (polpa bruta produziu a morte em 2, dentre 4 animais experimentados. 2. - Foram notadas diferenças pequenas, mas nitidas, na morfologia das inclusões do alastrim e da vacina, em material fixado no liquido de Helly, incluido em parafina e corado pela hematoxilina-eosina. Dizem elas respeito ao numero de inclusões encontradas em cada celula epidermica e às suas reações de coloração. 3. - As inclusões do alastrim, quando apresentam grandes dimensões, conservam-se unicas ou solitarias no citoplasma das celulas epidermicas do macaco rhesus, e coram-se em tonalidade que varia do azul escuro ao cinzento-azulado. Comtudo, em celulas que sofreram necrose, ou naquelas contendo 2 a 4 inclusões de pequenas dimensões, por vezes elas se mostram coradas em roseo. 4. - As inclusões da vacina, quando em faze adeantada de desenvolvimento, são multiplas nas celulas epidermicas do macaco rhesus e mostram, regularmente, uma policromatofilia caracteristica.1. - Vesicles and pustules containing numerous cytoplasmic inclusion bodies within the epidermal cells were regularly produced in monkeys (Macacus rhesus by intravenous inoculation either of alastrim virus or of recently prepared vaccine emulsion, no previous scarifications being required. Alastrim virus seems less virulent for this species of monkey than the virus of vaccinia is

  7. Determinação de metodologia para oisolamento de protoplastos de tangerina Cleópatra (Citrus reshni Hort.)

    OpenAIRE

    OLIVEIRA, R.P. de; Mendes,B.M.J.; Tulmann Neto,A.

    1995-01-01

    A hibridação somática via fusão de protoplastos vem sendo utilizada no melhoramento de porta-enxertos de citros em diversos países. Nos Estados Unidos, vários estudos demonstram a eficiência de procedimentos no isolamento e cultivo de protoplastos dessa frutífera. O presente trabalho foi realizado com o objetivo de avaliar o efeito do meio de cultivo de calos embriogênicos do porta-enxerto tangerina Cleópatra (Citrus reshni Hort.) sobre o isolamento de protoplastos, bem como sugerir alteraçõe...

  8. UTJECAJ SELENA NA PROIZVODNA OBILJEŽJA I BIOKEMIJSKE POKAZATELJE U KRVI NESILICA

    OpenAIRE

    Gajčević, Zlata; Kralik, Gordana; Šperanda, Marcela; Škrtić, Zoran; Matanić, Ivica

    2008-01-01

    Selen je esencijalni mikroelement potreban životinjama za rast i održavanje normalnih biokemijskih funkcija. Deficit selena u ptica, osobito uz paralelni nedostatak vitamina E, uzrokuje pojavu eksudativne dijateze i encefalomalacije, a utvrđen je i pad otpornosti, smanjenje proizvodnje, opločenosti i valivosti jaja, slabije operjavanje pilića i porast embrionalne smrtnosti. Istraživanjem je obuhvaćeno 480 nesilica hibrida Hy Line Brown u dobi od 26. do 30. tjedna, podijeljenih u četiri skupin...

  9. Diseño de una bicicleta polivalente mediante diseño generativo

    OpenAIRE

    NAVARRO PASCUAL, JOSÉ

    2016-01-01

    [ES] En el siguiente texto se proyectará la idea de una bicicleta hibrida y/o polivalente, es decir, una bicicleta que sirva para circular tanto por asfalto como en terreno arenoso, incluso con gravilla, y pueda ser igual de eficiente en ambos terrenos. Para ello estudiaremos los diferentes tipos de bicicleta existentes en la actualidad, o los modelos más comunes del mercado, así como su historia y evolución. Dentro de cada tipo de bicicleta estudiaremos sus características pri...

  10. La universidad nodo como modelo de vinculación universitaria

    OpenAIRE

    Mauricio Andión Gamboa

    2008-01-01

    A fin de comprender el sentido de los cambios en los modelos universitarios y el significado del concepto universidad nodo como institución universitaria en el marco de la sociedad red, en este ensayo se intentará explicar la morfología este modelo universitario y su sistema de operación. El articulo se estructura como un relato en donde la universidad nodo aparece como una organización hibrida y polimorfa articulada para funcionar como un alternativa para preservar la docencia y la investiga...

  11. Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization

    OpenAIRE

    Oliveira, M.; Guerra, M.; F. Bernardo

    2008-01-01

    Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH) para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%). Por meio do pr...

  12. Utjecaj tretmana sjemena na sadržaj ulja u zrnu

    OpenAIRE

    Šimić, Branimir; Krizmanić, Miroslav; Liović, Ivica; Miljić, Anto; Bilandžić, Marijan; Popović, Ruža

    2006-01-01

    Zrno suncokreta specifičnih je svojstava s obzirom na genotip, proces dorade sjemena, tretman i skladištenje a uslijed toga često dolazi do promjene kemijskog sastava zrna. Ovim istraživanjem utvrđena je promjena kemijskog sastava zrna (sadžaj ulja, %/ST) opadanje energije klijanja i klijavosti sjemena suncokreta, a ispitivanje je obavljeno na netretiranom (kontrola) i tretiranom sjemenu (T1 : Apron 35 DS + Geocid ST-35 i T2 : Apron 35 DS + Chinok 600FS). Analizirano je sjeme dva hibrida sunc...

  13. PSYCHOANALYSIS AND NEUROSCIENCE: A MAP OF THE DEBATES

    OpenAIRE

    MARCIA MORAES DAVIDOVICH

    2009-01-01

    Na presente dissertação elaboramos um mapa dos debates sobre a possibilidade de se articular Psicanálise e Neurociência, em que identificamos três grupos principais, cada um com seus próprios pressupostos, em função dos quais lhes atribuímos diferentes designações: hibridação, isolamento e interlocução. Posicionamo-nos a favor do terceiro grupo - interlocução - e contrariamente aos demais, deixando explícito, ao longo da dissertação, o motivo do nosso posicioname...

  14. Interculturalidade e literatura de almanaques: o caso do almanaque Abril (1975-2006) - Interculturality and literature of almanacs: the case of almanaque Abril (1975-2006)

    OpenAIRE

    Mateus H. F. Pereira

    2011-01-01

    Resumo No presente texto, procuramos refletir sobre a interculturalidade lançando o olhar para uma publicação que pode ser considerada um dos maiores sucessos editoriais do mercado brasileiro contemporâneo: o Almanaque Abril (1975-2006). Conforme mostraremos, a literatura de almanaque nos parece ser uma fonte privilegiada para a apreensão de certas “culturas híbridas”, uma vez que sua própria definição é caracterizada pelo hibridismo. Palavras-chave: literatura de almanaques; hibridação; Alma...

  15. Dinâmica populacional em populações de abelhas africanizadas (Appis mellifera L.) no nordeste brasileiro

    OpenAIRE

    Caroline Julio Moretti

    2014-01-01

    Em sua distribuição autóctone, as abelhas Apis mellifera apresentam diversas diferenciações morfológicas, comportamentais e ecológicas, que as possibilitam habitar os mais variados ambientes, apresentando grande diversidade de subespécies adaptadas a cada região. Com a introdução das abelhas africanas Apis mellifera scutellata no Brasil, em 1956, surgiram populações polí-hibridas denominadas Africanizadas, sendo que essas abelhas se tornaram interessantes para várias atividades econômicas e e...

  16. MENELUSURI IDENTITAS IKAN LELE DUMBO

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2013-12-01

    Full Text Available Lele dumbo merupakan salah satu ikan lele unggul yang budidayanya pernah mengalami perkembangan pesat di Indonesia. Secara umum, ikan lele dumbo dipercaya sebagai ikan lele hibrida hasil hibridisasi antara spesies ikan lele Afrika Clarias gariepinus dengan spesies ikan lele Taiwan C. fuscus. Tetapi, secara morfologis tampaknya ikan lele dumbo tidak berbeda dari strain-strain ikan lele Afrika C. gariepinus yang berikutnya diintroduksi ke Indonesia, sehingga para praktisi perikanan juga menduga bahwa ikan lele dumbo sebenarnya merupakan spesies ikan lele Afrika C. gariepinus. Dengan demikian, identitas ikan lele dumbo tetaplah belum jelas. Hal tersebut dikarenakan belum adanya penelitian yang mengeksplorasi karakteristik ikan lele dumbo secara ilmiah. Makalah ini merupakan ulasan penelusuran identitas ikan lele dumbo berdasarkan publikasi-publikasi yang relevan. Berdasarkan tinjauan terhadap beberapa bukti publikasi dalam ulasan ini, terindikasi bahwa ikan lele dumbo bukanlah ikan lele hibrida hasil hibridisasi antara ikan lele C. gariepinus dengan C. fuscus, tetapi tampaknya lebih merupakan spesies ikan lele Afrika C. gariepinus. Namun demikian, hal tersebut masih merupakan suatu indikasi, sehingga diperlukan penelitian-penelitian lebih lanjut untuk memastikan hal tersebut.

  17. Kajian Pengaruh Konfigurasi Mesin Penggilingan terhadap Rendemen dan Susut Giling beberapa Varietas Padi

    Directory of Open Access Journals (Sweden)

    Rokhani Hasbullah

    2009-10-01

    Full Text Available Rice is the staple food for Indonesian people so that the rice supply will be the barometer for thenational’s food security. In rice production, both of postharvest losses and low milling yield are still theproblems due to lack of postharvest handling. In this study, the effect of rice milling machine configurationon the milling yield and quality of some paddy varieties was examined. Paddy variety of Ciherang, Cibogoand Hybrid were milled using a configuration process of: 1 two husking and two polishing (2H-2P, 2 onehusking, one separating and one polishing (H-S-P, and 3 one husking, two separating and two polishing(H-2S-2P. The results show that the paddy varieties significanly affect milling yield and losses. However,rice milling machine configurations are not significantly affect the milling yield and losses. Paddy variety ofCibogo resulting in the highest milling yield (67.80 % compared to Ciherang (62.61 % and Hibrida (60.78%. Paddy variety of Cibogo resulting in the lowest losses (1.41 % compared to Ciherang (3.43 % andHibrida (3.03 %. The speed of milling process for configuration of 2H-2P, H-S-P and H-2S-2P were 228.1kg/hour, 295.6 kg/h and 263.2 kg/hr respectively.

  18. Study by Monte Carlo simulation of the absorbed dose in cells of breast cancer of the line MDA-MB231, due to sources of {sup 111}In, {sup 177}Lu and {sup 99m}Tc internalized in the nucleus. First results; Estudio por simulacion Monte Carlo de la dosis absorbida en celulas de cancer de seno de la linea MDA-MB231, debida a fuentes de {sup 11I}n, {sup 177}Lu y {sup 99m}Tc internalizadas en el nucleo. Primeros resultados

    Energy Technology Data Exchange (ETDEWEB)

    Rojas C, E. L.; Perez A, M., E-mail: leticia.rojas@inin.gob.mx [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2011-11-15

    The necessity to design innovative treatments and to diagnose the cancer early, has taken to investigate therapies at cellular and molecular level. The design of appropriate radio-molecules to these therapies makes necessary to characterize in way exhaustive radionuclides that they are of accessible production in our country and to study as distributing the dose at cellular level with bio-molecules glued them. In this context, was realized the present work. Using Monte Carlo simulation, the energy deposited in a geometric model of cells of breast cancer was obtained, MDA-MB231, due to different radionuclides. The energy deposited in the nucleus was evaluated, in the cytoplasm and in the membrane of the cell, using the simulation code Monte Carlo Penelope 2008. A punctual source was simulated in the center of the cell nucleus. In each case all the emissions of each radionuclide majors to 400 eV were simulated. The energies deposited by disintegration in the nucleus, cytoplasm, membrane of the cell and in a sphere of 2 cm surrounding the source (in eV) were: 4.30E3, 4.85E2, 1.07E2 and 3.29E4, correspondingly, for the {sup 111}In; 4.46E3, 3.76E3, 1.26E3 and 1.33E5 for the {sup 177}Lu and; 2.12E3, 2.58E2, 9.33E1 and 1.88E4 for the {sup 99m}Tc. We can conclude that if the union of these radionuclides happens to a compound that was internalized to the cell nucleus, the best for therapy at this level is the conjugate with the {sup 177}Lu, followed by that with {sup 111}In and in third place that with {sup 99m}Tc. (Author)

  19. Tyrosine, Tryptophan and Performance

    Science.gov (United States)

    1992-01-22

    a- e PCUMENTATION PAGE F .A q . 4_ Waq _III I u f I I 0’ND-A246 954 0 - Sdofr oATI I . nlpsti r0vn Amo OATU cOVsUD 22 Januarv 1992 ]Annual Jan. 9 -Dec...Indiana, May 15-17, 1991. Deng, M.H., Lynch H.J., Wurtman, R.J. and Lopez G.-Coviella, I. La diferenciacion de celulas de retinoblastoma...100 mg/kg) onou strreata4 25 THE EFFECT OF Ro 40-7592 ON SYSTOLIC BLOOD PRESSURE 220- 0-0 SAL + NET *- COMTI + NE LL 200 (fl T/ 0 V) 180 0 ± W M Q

  20. Aislamiento, purificación y caracterización de la nucleósido difosfato quinasa del hongo patógeno Candida albicans : Comparación con otras especies

    OpenAIRE

    Biondi, Ricardo M

    1996-01-01

    La enzima nucleósido difosfato quinasa (NDP quinasa) es ubicua y cataliza la síntesis de NTPs a partir de sus correspondientes NDPs y un NTP dador (fisiológicamente el ATP). La reacción sigue una cinética de ping-pong con la formación del intermediario de alta energía (P)NDP quinasa. Aparte de su rol fundamental en la síntesis de NTPs, se ha relacionado a esta enzima con a) la activación de proteínas G en la transducción de señales, b) la diferenciación de Drosophila y distintas líneas celula...

  1. Gestão organizacional sob a lógica da competência: aplicação na pequena empresa.

    Directory of Open Access Journals (Sweden)

    Mariana Musetti Munck

    2008-02-01

    Full Text Available Este estudo, sustentado nas premissas teoricas do modelo de gestão por competencias, objetiva explorar, descrever e mudar a sistematica de execução das atividades de uma pequena empresa de consultoria. Orientando-se por autores que propoem a operacionalização do conceito de competencias nas organizacões, construiram-se quadros de referencias para as celulas da organização de modo que houvesse mais transparencia e consistencia nas ações de gestão, e isso representa um grave problema das pequenas empresas. Os quadros são compostos de atributos como missão, atribuições, contribuições, indicadores de resultados e competencias, todos definidos com base nas estrategias e nos objetivos almejados pela organização. Ante a proposta de não apenas explorar e descrever, mas tambem de mudar, o estudo utilizou como orientação metodologica a pesquisa-ação. Esse metodo não se limita apenas a descrever uma situação, mas tambem pretende desencadear, em certos casos, mudancas no seio da coletividade envolvida. O proprio processo de busca e os resultados alcancados demonstraram, a principio, que a estruturação de uma empresa por competencias gera turbulencias iniciais, principalmente pela radicalidade da mudança (neste caso e pelas particularidades do conceito. A nova estrutura tem provocado mudanças culturais, sobretudo na açao dos gestores que tem agora informações claras sobre as atribuições e exigencias cabiveis a cada celula, bem como de seus respectivos indicadores de desempenho.

  2. Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro = Morte celular induzida pelo tamoxifeno em linfócitos humanos cultivados in vitro

    Directory of Open Access Journals (Sweden)

    Selma Candelária Genari

    2010-10-01

    Full Text Available Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3 and elderly women (58-77 years; n = 3 and cultivated for 24 or 48h, with or without TAM (20 ƒÊM. After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemicalanalysis evidenced higher frequency of apoptosis in treated cells, and the ultrastructural study revealed autophagic vacuoles, differing from the controls. In summary, the treated lymphocytes were affected by TAM, leading to cell death by apoptosis and autophagy.Muitos agentes quimioterapicos com potencial contra tumores solidos ou leucemias podem causar linfopenia. O Tamoxifeno (TAM e um agente antiestrogeno nao-esteroidal empregado no tratamento de cancer de mama feminino. O presente trabalho investigou a capacidade do TAM em induzir morte celular em linfocitos humanos cultivados in vitro. Oslinfocitos foram obtidos de mulheres jovens (25-30 anos; n = 3 e idosas (58-77 anos; n = 3 e cultivados por 24 ou 48h, com ou sem TAM (20 ƒÊM. Apos a cultura, foram analisadas a viabilidade celular, a resposta imunocitoquimica e a ultraestrutura. Os resultados indicam que o Tamoxifeno induziu morte celular em linfocitos de ambos os grupos, entretanto, as celulas das mulheres idosas apresentaram-se mais sensiveis ao tratamento. A analise imunocitoquimica mostrou maior frequencia de apoptose nas celulas tratadas e o estudo ultraestrutural revelou vacuolos autofagicos nos linfocitos expostos ao Tamoxifeno. Em conclusao, nosso estudo revelou que o TAM

  3. Isolamento e eficiência de plaqueamento de protoplastos de citros

    OpenAIRE

    COSTA MARIA ANGÉLICA PEREIRA DE CARVALHO; MOURÃO FILHO FRANCISCO DE ASSIS ALVES; MENDES BEATRIZ MADALENA JANUZZI

    2002-01-01

    As recentes ferramentas biotecnológicas para o melhoramento in vitro de citros incluem a hibridação somática por fusão de protoplastos. A otimização dos protocolos de isolamento, plaqueamento e cultura de protoplastos, fundamental para maior eficiência na produção de híbridos somáticos, foi avaliada em 11 variedades cítricas. As soluções enzimáticas testadas foram: 1. celulase Onozuka RS, 1%; macerase R-10, 1%, pectoliase Y-23, 0,2%; 2. celulase Onozuka R-10, 0,2 %; macerase R-10, 0,3%; drise...

  4. In silico analysis of the coffee transcriptome : identification of SNPs and inference of mechanisms of gene expression regulation

    OpenAIRE

    Ramon Oliveira Vidal

    2010-01-01

    Resumo: O café é uma das culturas mais importantes do mundo, sendo consumido mundialmente e com significativa participação na economia em países em desenvolvimento. Coffea arabica e Coffea canephora são responsáveis por 70% e 30% da produção comercial, respectivamente. Análise citogenética indicou que C. arabica é uma planta alotetraploide autógama formada por uma hibridação (1 milhão de anos atrás) entre os diplóides C. canephora e Coffea eugenioides. C. eugenioides é uma espécie silvestr...

  5. de vinculación universitaria

    Directory of Open Access Journals (Sweden)

    Mauricio Andión Gamboa

    2008-01-01

    Full Text Available A fin de comprender el sentido de los cambios en los modelos universitarios y el significado del concepto universidad nodo como institución universitaria en el marco de la sociedad red, en este ensayo se intentará explicar la morfología este modelo universitario y su sistema de operación. El articulo se estructura como un relato en donde la universidad nodo aparece como una organización hibrida y polimorfa articulada para funcionar como un alternativa para preservar la docencia y la investigación académica y protegerlas de las presiones de la globalización, a través del desarrollo de la extensión universitaria como función sustantiva y del compromiso con los valores de autonomía, libertad académica y responsabilidad social.

  6. A clínica, a epidemiologia e a epidemiologia clínica

    OpenAIRE

    Almeida Filho,Naomar de

    1993-01-01

    O presente estudo discute as múltiplas articulações conceituais e metodológicas entre a prática clínica e a epidemiologia, afirmando que o modo de produção do saber epidemiológico é inadequado para a formação do discurso clínico. Posteriormente ele avalia o grau de legitimidade episte-mológica da proposta de hibridação consolidada na chamada "epidemiologia clínica", especialmente no que diz respeito à tentativa de tornar técnica a decisão corrente em abos os campos do diagnóstico e da terapêu...

  7. Melhoramento genético da bananeira: estratégias e tecnologias disponíveis

    Directory of Open Access Journals (Sweden)

    Sebastião de Oliveira e Silva

    2013-09-01

    Full Text Available A banana cultivada 107 países, em uma área de 4,1 milhões de hectares e produção de 95 milhões de toneladas, é segunda fruta mais produzida do mundo. A bananeira é atacada por vírus (CMV e BSV, fungos (Sigatoka amarela e negra, mal-do-Panamá, bactéria (Moko, nematoide e insetos (Broca do rizoma. No entanto, por meio do melhoramento genético é possível obter resistência a maioria das pragas e doenças. O centro de origem de grande parte do germoplasma de Musa spp. é o Continente Asiático, onde são encontradas bananeiras diploides, triploides tetraploides, com genomas de Musa acuminta e M. balbisiana. No melhoramento de banana, feito principalmente para resistência às doenças, são usados os seguintes métodos: introdução e seleção de clones; hibridação (cruzamentos de diploides com diploides, triploides com diploides e diploides com tetraploides; duplicação de cromossomos; mutação e transgenia. Os métodos que envolvem hibridação, embora sejam os mais usados, apresentam limitações como a partenocarpia, a esterilidade; o número variável de ploidia e a baixa produção de sementes. Todo material produzido no programa, é depois avaliado nas regiões produtoras de banana. Atualmente novas técnicas de melhoramento, baseadas em informações genéticas de Musa spp. estão sendo incrementadas.

  8. Caracterização anatômico-fisiológica da compatibilidade reprodutiva de ameixeira-japonesa

    Directory of Open Access Journals (Sweden)

    Daniela de Conti

    2013-09-01

    Full Text Available O objetivo do trabalho foi caracterizar fisiologicamente a compatibilidade reprodutiva de sete cultivares de ameixeira-japonesa (Prunus salicina Lindl por meio de hibridações, análise de frutificação efetiva e do crescimento de tubos polínicos (CTP. O frui set foi determinado 40 dias após as hibridações controladas, realizadas a campo. O grau de compatibilidade foi avaliado invivo, para determinar o CTP. A cv. América apresentou bom fruit set quando polinizada com as cvs. Rosa Mineira (26,7%, Amarelinha (8,7% e Reubennel (12,7%. Os cruzamentos 'Gulf Blaze' x 'Gulf Rubi', 'Gulf Rubi' x 'Gulf Rubi' e 'Gulf Rubi' x 'Gulf Blaze' obtiveram um fruit set de 11,36%, 3,84% e 9,94%, respectivamente. Na polinização em laboratório, os tubos polínicos atingiram o óvulo ou ovário nesses cruzamentos, com exceção da autopolinização da 'Gulf Rubi'. No campo, não houve frutificação efetiva na autopolinização da 'América' e 'Gulf Blaze' e no cruzamento da 'América' x 'Pluma 7'. Os tubos polínicos nesses cruzamentos não chegaram a atingir o óvulo, com exceção da autopolinização da 'Gulf Blaze'. Concluiu-se que apenas os cruzamentos entre 'América' x 'Pluma 7' são incompatíveis, e a cultivar América é autoincompatível.

  9. Diagnóstico citogenético de pacientes com retardo mental idiopático Cytogenetic diagnosis of patients with idiopathic mental retardation

    Directory of Open Access Journals (Sweden)

    Natália Duarte Linhares

    2012-02-01

    Full Text Available O retardo mental é uma condição presente em 2% a 3% da população e mais da metade dos casos ainda são considerados idiopáticos. Sua etiologia é heterogênea e as anomalias cromossômicas têm importante contribuição. A aplicação de técnicas de citogenética clássica e de citogenética molecular tem permitido o diagnóstico preciso em muitos casos, proporcionando melhor acompanhamento clínico e aconselhamento genético. Este trabalho tem como objetivo informar sobre os principais exames atualmente disponíveis para a investigação de rearranjos cromossômicos em pacientes com retardo mental idiopático, incluindo cariótipo com bandeamento G, hibridação in situ fluorescente (FISH, cariotipagem espectral (SKY, amplificação de múltiplas sondas dependente de ligação (MLPA e hibridação genômica comparativa em array (array-CGH.Mental retardation is a condition that affects 2%-3% of the population and more than half of the cases are still deemed idiopathic. Its etiology is heterogeneous and chromosome abnormalities play a significant role. The application of classical cytogenetic and molecular cytogenetic techniques has enabled accurate diagnosis in several cases, which allows better clinical monitoring and genetic counseling. This paper aims at informing about the major tests currently available to investigate chromosome abnormalities in patients with idiopathic mental retardation, including GTG-banded karyotyping, fluorescence in situ hybridization (FISH, spectral karyotyping (SKY, multiplex ligation-dependent probe amplification (MLPA and array-comparative genomic hybridization (array-CGH.

  10. Algumas investigações sobre Schizotrypanum de Quirópteros

    Directory of Open Access Journals (Sweden)

    Emmanuel Dias

    1939-01-01

    Full Text Available Os autores referem as especies de morcegos de diversos países americanos, emque foi pesquisada no sangue a presença de hemoflagelados. Tripanosomas com a morfologia das formas sanguicolas de Schizotrypanum foram encontrados, tendo sido colhidos dados novos sobre a distribuição geografica e a incidencia da infecção em diferentes especies de morcegos. Sómente nos tecidos do Phyllostomus hastatus puderam ser encontradas as formas de multiplicação do flagelado. Alimentando-se exemplares de Triatoma infestans em Eumops bonariensis beckeri infectado, foi obtido desenvolvimento intestinal do parasito. Por inoculação das vinchucas assim infectadas em camondongo, conseguiu-se facilmente transmissão da infecção a este animal. Inoculando-se cultura do Schizotrypanum de Phyllostomus hastatus em coelho, por via subcutanea, verificaram-se no ponto de inoculação, 5 dias depois, celulas contendo tipicas formas de leishmania. São assinalados fatos observados sobre a associação demorcegos com Hemipteros sugadores (Reduvideos e Cimicideos, os quais, pela circunstancia de se poderem infectar pelo flagelado, são os possiveis transmissores da infecção a estes mamiferos.

  11. Criopreservação de formas de cultura do Trypanosoma cruzi Cryopreservation of Trypanosoma cruzi culture form

    Directory of Open Access Journals (Sweden)

    Lúcia Maria C. Galvão

    1981-09-01

    Full Text Available Formas de cultura de diferentes cepas do T.cruzi foram submetidas a vários processos de criopreservação. As percentagens de recuperação, avaliadas pela motilidade dos parasitas, foram consideradas como adequadas com algumas das técnicas empregadas, variando entre 60 a 80%. A estabilidade das características biológicas do material criopreservado foi investigada através do estudo das curvas de crescimento e diferenciação em meio acelular, infectividade para celulas de cultura de tecido ("Vero", diferenciação intracelular em cultura de tecido assim como infectividade e curso da infecção em animais de laboratório. De um modo geral essas características nao foram significativamente alteradas no material congelado e estocado por diferentes períodos de tempo.A systematic study of the cryopreservation of T. cruzi culture forms was per formed using different parasite strains and freezing methods. The recovery rates with some of the methods as evaluated by motility of the thawed parasites were fairly high (60-80%. The following aspects have been used to investigate the stability of the parasites' biological characteristics atter cryopreservation: growth and differentiation in acelular medium, infectivity to tissue culture "Vero" cells, intracellular differentiation and infectivity to animals. Those characteristics had not been significantly changed by the cryopreservation procedures.

  12. [Cervical ischaemic neuronopathy and cardioembolism: another cause of man-in-the-barrel syndrome].

    Science.gov (United States)

    Gonzalez-Usigli, H; Gandarilla, A; Garcia, J J; Serrato, J; Estrada, N

    2016-12-16

    Introduccion. Los infartos de la medula espinal ocurren aproximadamente en el 1% de todos los infartos del sistema nervioso. El cardioembolismo es una causa rara. Las areas comunmente afectadas en infartos medulares son zonas de circulacion limitrofe en la region dorsal o lumbar; sin embargo, se han descrito previamente infartos medulares en la zona cervical asociados a la diseccion de arterias vertebrales. Caso clinico. Se presenta un nuevo caso de sindrome del hombre en el barril asociado con fibrilacion auricular durante un infarto agudo de miocardio, que causo una neuronopatia cervical isquemica (infarto de astas anteriores). Conclusiones. Los hallazgos clasicos de isquemia medular cervical son tetraplejia aguda, disfuncion vesical, intestinal y cardiovascular, asi como perdida de la sensibilidad disociada. La colateralizacion incompleta y las caracteristicas anatomicas de la arteria espinal anterior en la medula cervical hacen de la porcion C3-C5 la region mas vulnerable de daño isquemico, ya que la irrigacion de las arterias radiculares se inicia debajo de C5 y la fisiopatologia de la lisis rapida de un coagulo fresco produce unicamente daño bilateral de las celulas de las astas neuronales.

  13. [First case described of isolated, complete and fluctuating cranial nerve III palsy heralding multiple myeloma].

    Science.gov (United States)

    León-Ruiz, Moisés; Benito-León, Julián; Sierra-Hidalgo, Fernando; García-Soldevilla, Miguel Ángel; Izquierdo-Esteban, Laura; Tejeiro-Martínez, José; Cabrera-Valdivia, Francisco; García-Albea Ristol, Esteban

    2015-02-01

    Introduccion. El mieloma multiple es la neoplasia de celulas plasmaticas mas frecuente. Al ser incurable, el tratamiento persigue obtener el mayor tiempo de supervivencia libre de clinica. Constituye una causa extremadamente rara de afectacion de los nervios craneales y es producido habitualmente por un plasmocitoma intracraneal. Presentamos un caso de mieloma multiple, que asociaba un plasmocitoma intracraneal y que comenzo clinicamente con paralisis aislada, completa y fluctuante del III nervio craneal. Caso clinico. Mujer de 63 años que acudio a urgencias por presentar un cuadro clinico oscilante, consistente en diplopia binocular horizontal y, posteriormente, cefalea. La exploracion neurooftalmologica revelo una paralisis completa del III nervio craneal derecho. Se solicito una tomografia axial computarizada craneal urgente, que revelo multiples lesiones osteoliticas diploicas, asociando una de ellas componente de partes blandas en la hendidura esfenoidal derecha. La paciente fue ingresada, y se le diagnostico posteriormente un mieloma multiple IgA-kappa. Tras recibir induccion quimioterapica y ser sometida a un trasplante autologo de progenitores hematopoyeticos, alcanzo la remision completa. Conclusiones. El mieloma multiple es un trastorno raro de los nervios craneales, una causa muy infrecuente de paralisis aislada y completa del III nervio craneal y menos aun fluctuante, y no se ha encontrado ningun caso publicado con este inicio clinico. Tener en cuenta las posibles manifestaciones neurooftalmologicas del mieloma multiple puede contribuir a un diagnostico precoz y a una incidencia positiva sobre el curso de esta enfermedad.

  14. [Advances in the management of neonatal hypoxia].

    Science.gov (United States)

    Riesgo, Rudimar dos Santos; Becker, Michele M; Ranzan, Josiane; Winckler, Maria Isabel B; Ohlweiler, Lygia

    2013-09-06

    Introduccion. Durante el nacimiento, ocurren cambios fisiologicos en practicamente todos los organos del niño, incluyendo el sistema nervioso central. En esta fase de transicion, es posible un cierto grado de hipoxemia, en general bien tolerado por el neonato. Sin embargo, si la hipoxia neonatal es muy intensa y continuada, puede instalarse una encefalopatia neonatal, lo que caracteriza una situacion critica para el recien nacido. Su abordaje adecuado es imprescindible para garantizar un buen pronostico a largo plazo. Desarrollo. Se actualizan las informaciones acerca de la hipoxia neonatal y se revisan publicaciones recientes acerca de los avances en su abordaje a traves de la medicina basada en evidencias. Conclusiones. La encefalopatia neonatal se puede clasificar desde el punto de vista clinico en tres niveles de intensidad. Usualmente, los casos leves tienen un buen pronostico, los casos de intensidad moderada tienen un 30% de posibilidad de secuelas y los de intensidad grave tienen mas del 70% de mortalidad, pero practicamente todos los supervivientes tendran secuelas. Los avances ocurrieron en dos areas: en el diagnostico, con nuevas tecnicas de EEG y RM, y en el tratamiento, con la aparicion de la hipotermia terapeutica. Existe la posibilidad de un uso futuro para la terapia con celulas madre. El pronostico depende de la clasificacion clinica, de los datos de neuroimagen y del EEG.

  15. [Myelopathy secondary to an aneurysmal bone cyst of thoracic spine].

    Science.gov (United States)

    Navas-García, Marta; Penanes, Juan Ramón; Fraga, Javier; Sola, Rafael G

    2016-02-01

    Introduccion. Los quistes oseos aneurismaticos espinales son lesiones osteoliticas benignas muy infrecuentes constituidas por cavidades hematicas limitadas por septos osteoconectivos y celulas gigantes tipo osteoclastos. Clinicamente se manifiestan con dolor local, sintomas neurologicos secundarios a compresion medular, asi como fracturas, deformidades e inestabilidad vertebral. Presentamos un caso de quiste oseo aneurismatico espinal dorsal con sintomatologia neurologica, tratado mediante una reseccion microquirurgica completa, sin secuelas neurologicas asociadas. Caso clinico. Mujer de 47 años, sin antecedentes traumaticos previos, valorada por presentar un cuadro de parestesias de los miembros inferiores de semanas de evolucion. El estudio radiologico de resonancia magnetica dorsal demostro la existencia de una lesion litica de bordes bien delimitados y esclerosis marginal en D4, con afectacion de los elementos posteriores vertebrales y compresion del cordon medular subyacente. La lesion fue extirpada en su totalidad, con desaparicion de la clinica sensitiva tras la intervencion. El diagnostico anatomopatologico definitivo fue quiste oseo aneurismatico espinal. Conclusion. A pesar de su baja incidencia, los quistes oseos aneurismaticos espinales deben considerarse, en el diagnostico diferencial de los tumores oseos espinales, como una posible causa de mielopatia compresiva subaguda o cronica. La reseccion tumoral completa se considera el tratamiento de eleccion, el cual con frecuencia es curativo y asocia un buen pronostico del paciente a largo plazo.

  16. Modeling and simulation of PEM fuel cell's flow channels using CFD techniques

    Energy Technology Data Exchange (ETDEWEB)

    Cunha, Edgar F.; Andrade, Alexandre B.; Robalinho, Eric; Bejarano, Martha L.M.; Linardi, Marcelo [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)]. E-mails: efcunha@ipen.br; abodart@ipen.br; eric@ipen.br; mmora@ipen.br; mlinardi@ipen.br; Cekinski, Efraim [Instituto de Pesquisas Tecnologicas (IPT-SP), Sao Paulo, SP (Brazil)]. E-mail: cekinski@ipt.br

    2007-07-01

    Fuel cells are one of the most important devices to obtain electrical energy from hydrogen. The Proton Exchange Membrane Fuel Cell (PEMFC) consists of two important parts: the Membrane Electrode Assembly (MEA), where the reactions occur, and the flow field plates. The plates have many functions in a fuel cell: distribute reactant gases (hydrogen and air or oxygen), conduct electrical current, remove heat and water from the electrodes and make the cell robust. The cost of the bipolar plates corresponds up to 45% of the total stack costs. The Computational Fluid Dynamic (CFD) is a very useful tool to simulate hydrogen and oxygen gases flow channels, to reduce the costs of bipolar plates production and to optimize mass transport. Two types of flow channels were studied. The first type was a commercial plate by ELECTROCELL and the other was entirely projected at Programa de Celula a Combustivel (IPEN/CNEN-SP) and the experimental data were compared with modelling results. Optimum values for each set of variables were obtained and the models verification was carried out in order to show the feasibility of this technique to improve fuel cell efficiency. (author)

  17. [Acute optic neuropathy: differential diagnoses].

    Science.gov (United States)

    Buompadre, María Celeste

    2013-09-06

    Introduccion. La alteracion funcional del nervio optico se caracteriza por un deficit en la agudeza visual, en la vision cromatica y en el campo visual, defecto pupilar aferente y, en algunos casos, edema del nervio o atrofia y palidez. Objetivo. Describir el espectro de neuropatias opticas agudas, su clinica, diagnostico y tratamiento, con mayor interes en aquellas de presentacion en la edad pediatrica. Desarrollo. La neuritis optica puede ser monofasica, recurrente o el componente de un cuadro desmielinizante polisintomatico. El objetivo del tratamiento es reducir el numero y la gravedad de los ataques y prevenir discapacidad. La infecciosa es secundaria a diferentes microorganismos (bacterias, virus, hongos y protozoos). El tratamiento depende de la etiologia. La isquemica anterior no arteritica o idiopatica es la forma mas frecuente y es secundaria a enfermedad de pequeños vasos (ciliares posteriores). La neuropatia optica hereditaria o de Leber representa una causa importante de afectacion visual cronica y se caracteriza por la afectacion selectiva de las celulas ganglionares de la retina. Hasta el momento, la terapia solo es de apoyo. En el papiledema asociado a hipertension endocraneal, la agudeza visual generalmente se conserva pero existe aumento de la mancha ciega. El tratamiento se basa en disminuir la hipertension y el factor etiologico si existe. Conclusiones. Las neuropatias opticas agudas constituyen un amplio grupo de entidades, de etiologia diversa y con un pronostico visual variable. La presencia de signos del examen neurologico, fondo de ojo y neuroimagenes pueden orientar hacia el diagnostico y tratamiento oportuno.

  18. Expresión episomal de toxina de pertussis genéticamente inactivada en Bordetella pertussis

    Directory of Open Access Journals (Sweden)

    Ernesto Marcos

    2010-01-01

    Full Text Available Bordetella pertussis es una bacteria Gram negativa, la cual constituye el agente etiologico de la tos ferina. La enfermedad se desencadena por el efecto conjunto de una serie de factores de virulencia expresados por la bacteria, los cuales se encuentran regulados por el sistema bvg. Uno de los factores de virulencia mas importantes es la toxina de pertussis, razon por la cual, se emplea de forma inactivada como el componente principal de las vacunas acelulares contra la enfermedad. La toxina de pertussis posee una estructura del tipo A-B compuesta por seis polipeptidos codificados en un operon unico. El polipeptido S1 constituye la subunidad enzimaticamente activa, la cual cataliza la transferencia de ADP-ribosa del NAD a la subunidad ALPHA de las proteinas G en celulas eucariotas, lo cual genera una serie de efectos biologicos dentro de los que se incluye: sensibilizacion a histamina, incremento de la secrecion de insulina y efectos inmunoestimuladores e inmunosupresores. El presente trabajo describe los procedimientos realizados para la obtencion de cepas de Bordetella pertussis productoras de elevadas concentraciones de toxina pertusica atenuada geneticamente. Para esto, se realizaron las sustituciones aminoacidicas Arg9 por Lys y Glu129 por Gly de la subunidad S1. El operon de la toxina de pertussis mutada se clono en un vector de amplio rango de hospedero bajo la regulacion de un promotor de expresion temprana (fhaB. Los clones obtenidos pudieran ser empleados como sistemas de expresion para produccion de vacunas acelulares en Cuba.

  19. [Neurological disorders and the blood-brain barrier. Strategies and limitations for drug delivery to the brain].

    Science.gov (United States)

    Domínguez, Alazne; Álvarez, Antonia; Suárez-Merino, Blanca; Goñi-de-Cerio, Felipe

    2014-03-01

    Introduccion. La incidencia de enfermedades del sistema nervioso central (SNC) aumenta a causa del envejecimiento de la sociedad. Desgraciadamente, los tratamientos clasicos para tratarlas no resultan efectivos debido a la presencia de la barrera hematoencefalica. Objetivo. Abordar las propiedades de la barrera hematoencefalica que impiden el transporte de los farmacos al cerebro y las principales estrategias para tratar las afecciones neurologicas. Desarrollo. La barrera hematoencefalica esta compuesta principalmente por un endotelio vascular especializado y las celulas de la glia. Esta constituye una herramienta a disposicion del organismo para aislar al SNC del resto del cuerpo. Sin embargo, tambien supone un impedimento para que muchos farmacos alcancen su diana en el cerebro. Conclusiones. Para poder tratar las afecciones neurologicas, los farmacos deben ser capaces de alcanzar el cerebro. Los agentes terapeuticos pueden diseñarse para que sean capaces de atravesar esta barrera, o bien facilitar su entrada mediante el uso de sistemas de liberacion. Para evaluar la efectividad de los tratamientos dirigidos a enfermedades del SNC, se emplean los modelos animales de enfermedades neurologicas asi como modelos in vitro de barrera hematoencefalica.

  20. Linfoma primario del sistema nervioso central en una paciente con lupus eritematoso sistémico

    Directory of Open Access Journals (Sweden)

    C. N. Pisoni

    2003-06-01

    Full Text Available Se presenta una paciente de 36 años con diagnóstico de lupus eritematoso sistémico tratada con prednisona y ciclofosfamida que se internó por cefalea, hemiparesia y hemianestesia braquiocrural derecha de dos semanas de evolución. Se realizó una tomografía computada y una resonancia magnética nuclear de cerebro que mostraron una lesión nodular frontal izquierda. Se efectuó una biopsia a cielo abierto de la lesión cerebral cuyo diagnóstico histopatológico fue linfoma B de celulas grandes, difuso. Se inició radioterapia, no completó el tratamiento por complicaciones y falleció. Son muy pocos los casos publicados de linfoma primario del sistema nervioso central asociado a lupus eritematoso sistémico.A 36 year-old woman with systemic lupus erythematosus was admitted to our hospital with headache, brachiocrural hemiparesis and hemianesthesia. She had been treated with prednisone and cyclophosphamide. CT scan and MRI revealed a 15 mm nodular mass enhanced with gadolinium in left frontal convexity. CNS biopsy was performed and a diffuse large B-cell lymphoma was diagnosed. She was treated with radiation therapy without response and died. There are few reports of erythematosus systemic lupus associated with primary central nervous system lymphoma.

  1. Formalization of common power and efficiency definitions for energy-converting intracellular biochemical processes

    Energy Technology Data Exchange (ETDEWEB)

    Santillan, M.; Angulo-Brown, F.; Chavoya-Aceves, O. [Instituto Politecnico Nacional, Mexico, D. F. (Mexico)

    2001-04-01

    The definitions of power and efficiency for energy-converting intracellular biochemical processes, introduced by Caplan and Essig are studied. These definitions are recovered in the present work with the formalism of De Groot and Mazure for First-Order Irreversible Thermodynamics, rather than the formalism of Prigogine, as done by Caplan and Essig. The approach here employed permits to keep track of all the assumptions in a more clear manner, and to get rid of a very strong restriction in the approach of Caplan and Essig which assumes that the chemical potentials are homogeneous inside the cell. [Spanish] Se estudian las definiciones de potencia y eficiencia para procesos bioquimicos intracelulares convertidores de energia, introducidas por Caplan y Essig. En el presente trabajo, dichas definiciones se recuperan usando el formalismo de De Groot y Mazur para la termodinamica irreversible de primer orden, en vez del formalismo de Prigogine, empleado por Caplan y Essig. El punto de vista empleado en el presente manuscrito permite seguir las suposiciones hechas de una manera mas clara, ademas de que hace innecesaria una suposicion bastante fuerte usada por Caplan y Essig, la cual da por hecho que los potenciales quimicos son homogeneos en el interior de la celula.

  2. Divergência genética entre acessos e cultivares de mamoneira por meio de estatística multivariada Genetic divergence on castor bean accesses and cultivars through multivariate analysis

    Directory of Open Access Journals (Sweden)

    Mauro Nóbrega da Costa

    2006-11-01

    Full Text Available O objetivo deste trabalho foi avaliar a divergência genética entre acessos e cultivares de mamoneira (Ricinus communis L. e utilizá-la como critério na escolha de genitores que viabilizem, a partir de hibridações, a formação de populações segregantes. Os tratamentos foram representados pelos acessos BRA 4871, BRA 2968, BRA 5550 e BRA 7722 Papo-de-gia, e as cultivares BRS 188 Paraguaçu, BRS 149 Nordestina, IAC-80, Mirante-10 e Pernambucana Melhorada. As características analisadas foram: início do florescimento (FR, número de racemos por planta (NRP, comprimento efetivo do racemo primário (CR, altura de planta (AP, potencial produtivo (PP e teor de óleo nas sementes (TO. A divergência genética foi estimada por meio de estatística multivariada, com base em variáveis canônicas e análise de agrupamento, tendo-se empregado a distância euclidiana média. Houve a formação de dois grupos: o grupo I formado por oito genótipos e o grupo II por apenas um genótipo, a cultivar Mirante-10. Apesar de a cultivar Mirante-10 ter sido a mais divergente, não deve ser recomendada para hibridação, por sua baixa média de desempenho. As demais cultivares também apresentam restrições para hibridação, por serem bastante similares. As variáveis que mais contribuíram para a divergência genética foram FR, AP, TO e CR.This work aimed to evaluate genetic divergence among castor bean (Ricinus communis L. cultivars, in order to enable the choice of parents which make the formation of segregating populations possible. Accesses BRA 4871, BRA 2968, BRA 5550 and BRA 7722 Papo-de-gia, and cultivars BRS 188 Paraguaçu, BRS 149 Nordestina, IAC-80, Mirante-10 and Pernambucana Melhorada were evaluated. Characteristics analyzed were: days to flowering, number of racemes per plant, length of pistillate region of main raceme, plant height, potential yield, and seed oil content. The genetic divergence among accesses and cultivars was studied by

  3. Cytogenetics of Triatominae: III - A study on male sterility induced through hybridization of Triatoma sórdida and Triatoma pseudomaculata

    Directory of Open Access Journals (Sweden)

    Giorgio Schreiber

    1975-08-01

    Full Text Available Males from bilateral crosses between Triatoma sórdida and Triatoma pseudomaculata were unable to give offspring, as shown by subsequent backcrosses (BC between hybrid males and parental females. This kind of sterility indueed through interspecific hybridization seems to be due to lack of sperm migration from the bursa copulatrix to the spermateca, thus suggesting primarily failure on the part of hybrid males to produce and/or to incorporate male accessory secretions into the spermatophore bulb. Addicional proof that sterility induced in hybrid males is at the sperm level has been afforded by the spermatogenesis herein studied. The anomalous processes like; 1 prophases of spermatogonia with the chromosomes scattered in the cytoplasm, 2 first metaphases with unpaired tetrades, 3 spermatids differing in size and 4 spermatozoa of abnormal shape and generdlly of giant size, can be taken as an indicator of the degree of departure from the normal course of spermatogenesis.Os machos provenientes de cruzamentos bilaterais entre Triatoma sórdida e Triatoma pseudomaculata revelaram a incapacidade de produzir progênie, como se demonstra nos retrocruzamentos de machos híbridos com fêmeas das espécies paternas. Esta modalidade de esterilidade induzida através da hibridação interespecífica, parece ser devida à ausência de migração do esperma, determinada pela incapacidade dos híbridos machos produzirem a secreção opaca em suas glândulas acessórias e/ou incorporá-la à luz do espermatóforo. A prova adicional de que a incompatibilidade induzida em triatomíneos, através da hibridação inter específica, está no nível do esperma é fornecida pelo tipo anômalo de espermatogênese em híbridos aqui descrito, mostrando deste modo que: as prófases espermatogoniais têm os núcleos disruptos e os cromossomos espelhados no citoplasma. As primeiras metáfases meióticas têm as tétrades desemparelhadas. Os grupos de cromatídes assim obtidos

  4. Melhoramento do cafeeiro: XLIII. seleção de cafeeiros resistentes ao bicho-mineiro Coffee breeding: XLIII. selection of coffee trees resistant to the leaf miner (Perileucoptera coffeella

    Directory of Open Access Journals (Sweden)

    Oliveiro Guerreiro Filho

    1990-01-01

    Full Text Available Estudaram-se diversos parâmetros agronômicos em progênies oriundas de potinização aberta e de hibridações envolvendo os cafeeiros C 1195-5-6-1 e C 1195-5-6-2, resistentes ao bicho-mineiro (Perileucoptera coffeella. Ambos apresentam florescimento abundante e precocidade de maturação, características da espécie Coffea racemosa, da qual se originaram por retrocruzamentos com C. arabica. Hibridações desses dois cafeeiros foram realizadas com os cafés Icatu, Catimor, Híbrido de Timor e Catuaí. Os três primeiros, derivados de retrocruzamentos de C. canephora com C. arabica, são resistentes ao agente da ferrugem (Hemileia vastatrix e, o último, cultivar de grande expressão econômica, tem porte reduzido e elevada capacidade produtiva. A análise das progênies realizada em quatro ensaios revelou que todos esses atributos, presentes em tão diverso germoplasma, são geneticamente transmissíveis e se encontram nas progênies investigadas, em associações diversas. Os dados de produção e o fato de não existirem associações antagônicas entre esses atributos sugerem a possibilidade do desenvolvimento de cultivares produtivos, resistentes ao bicho-mineiro e com outras características desejáveis, principalmente resistência ao agente da ferrugem.Several agronomic characteristics were studied in progenies from open pollinations and hybridizations of coffee trees C1195-5-6-1 and C1195-5-6-2. Both, resistant to the leaf miner (Perileucoptera coffeella, bloom profusely and are early ripening, characteristics of Coffea racemosa from which they originated via backcross to C. arabica. Hybrids of these two individuals with Icatu, Catimor, Híbrido de Timor and Catuaí cultivars were evaluated. The first three cultvars, C. canephora x C. arabica backcross derivatives, are resistant to the leaf rust (Hemileia vastatrix and the last a leading C. arabica cultivar has short stature and high yield capacity. The study of the progenies

  5. Pertunjukan Teater Karo Hip Hop Kontemporer KAI

    Directory of Open Access Journals (Sweden)

    Silvia Anggreni Purba

    2013-11-01

    Full Text Available Pertunjukan ini berawal dari sebuah ide untuk mengkolaborasikan tradisi Karo dengan budaya populer. Dengan cara seperti ini pertunjukan bisa dinikmati tanpa batasan bahasa dan budaya. Proses menggabungkan dua budaya yang berbeda merupakan bentuk budaya hibrida dan terjadi akibat proses globalisasi. Melalui proses pengendapan pengamatan dan kesan yang kuat, pertunjukan ini dibawa ke dalam bentuk Hip Hop. Pertunjukan ini merupakan bagian dari sebuah tragedi modern dengan karakter destruktif, mengeksplorasi emosi dan menyampaikannya kepada penonton. Eksplorasi budaya Karo dan tari Hip Hop sebagai bahasa simbol mampu memperkuat kata-kata. Gerak tidak diungkapkan dengan kata lisan tetapi disajikan melalui gerak tari Hip Hop. Penafsiran legenda dan teks ke dalam gerak, melalui proses pelatihan di laboratorium sebagai proses pencarian dan eksperimentasi diwujudkan dengan mempertimbangkan unsur-unsur dasar dari Hip Hop, unsur budaya Karo dan tontonan. Karo Hip Hop diharapkan menjadi bentuk estetika teater modern yang diinginkan tanpa kehilangan tradisi.Kata kunci: Tari Karo kontemporer, Hip-hop, budaya hibridaABSTRACTPertunjukan Teater Karo Hip Hop Kontemporer KAI. The performance of Karo Theater collaborated with Hip Hop stems from a simple idea to collaborate Karo cultural traditions with popular culture. The performances can be enjoyed without having limitation on the language and culture. The process of combining two different cultures is a form of hybrid culture, and it may occur due to the globalization process. Through the process of deposition of the observations and strong impression, this performance is then brought into the form of Hip Hop as a preferred form which is energetic, personal and global. This performance is part of a modern tragedy with its destructive character which has explored the emotion and has presented it to the audiences. The exploration of Karo cultural tradition and Hip Hop dance as a language of symbols is able to

  6. Comunicação transnacional mediada por computador: uma análise discursiva das identidades de futuros professores de inglês no Facebook

    Directory of Open Access Journals (Sweden)

    Bárbara Cristina Gallardo

    Full Text Available Tomando potencialmente inovadoras as oportunidades de Comunicação Mediada por Computador no âmbito da formação de professores de inglês, o objetivo deste artigo é analisar as representações da identidade nacional constituídas em um contexto virtual. Duas estudantes de Letras estabeleceram contato com estrangeiros no Facebook e com eles interagiram em língua inglesa. Utilizou-se a Análise Crítica do Discurso para desvendar a organização linguística e ideológica dos discursos nacionais acionados na transição para o contexto transnacional online. Os resultados sugerem a hibridação de estratégias do discurso de contextos offline com os recursos multimodais dos contextos online. Os sujeitos adotaram estereótipos oriundos de centros geradores das dinâmicas de globalização, tanto em escala regional (sudeste do Brasil como mundial (os EUA.

  7. SEMIOLOGIA DA PUBLICIDADE: A MENSAGEM PUBLICITÁRIA COMO PRINCIPAL MERCADORIA.

    Directory of Open Access Journals (Sweden)

    Deborah Veviani da Silva

    2013-12-01

    Full Text Available Pensar a própria mensagem publicitária enquanto mercadoria é ao mesmo tempo instigante e desafiador, uma vez que tão cheia de signos e quase sempre elaborada dentro de um viés hedonista, o anúncio publicitário se traveste de arte para vender os produtos de uma determinada marca. Evidentemente esse objetivo é de fácil apreensão, mas a hibridação entre estética e discurso camufla a verdadeira mercadoria que se está vendendo: a mensagem publicitária em si. Essa linha de pensamento se confirma quando ao se analisar uma determinada campanha, percebe-se que caso a temática seja alterada, ou seus produtos sejam alternadamente substituídos por outros (da mesma marca, obviamente, ainda assim pode-se verificar a assinatura da marca. Quase como uma espécie de gene semântico, a marca consegue passar e replicar sua mensagem embutida num discurso publicitário, cuja linguagem se adequa a uma temática que lhe dá forma, embora o conteúdo seja inalterado.

  8. Chromosomal differentiation between populations of Oligosarcus hepsetus (Teleostei, Characidae from small tributaries at opposite margins of the Paraíba do Sul river (Brazil

    Directory of Open Access Journals (Sweden)

    Liano Centofante

    2006-11-01

    Full Text Available Cytogenetic analyses were carried out in two populations of Oligosarcus hepsetus from tributaries at opposite margins of the Paraíba do Sul river. The same diploid number was observed in both populations (2n=50, but they showed remarkable differences related to karyotype formula and distribution of rDNA sites as revealed by silver nitrate staining and in situ hybridization with 18S probes. The results suggested that the main channel of the Paraíba do Sul river acted as barrier to gene flow between populations.Análises citogenéticas foram realizadas em duas populações de Oligosarcus hepsetus provenientes de riachos de margens opostas do rio Paraíba do Sul. O número diplóide foi o mesmo para ambas as populações (2n=50, porém diferenças significativas foram observadas com relação à fórmula cariotípica e distribuição dos sítios de DNAr detectados pela impregnação por prata e pela hibridação "in situ" com sonda 18S. Estes resultados sugerem que a calha principal do rio Paraíba do Sul estaria atuando como uma barreira para o fluxo gênico entre essas populações.

  9. Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus Fusão de protoplastos com microprotoplastos irradiados como ferramenta para painel híbrido de radiação em citros

    Directory of Open Access Journals (Sweden)

    Claudine Maria de Bona

    2009-12-01

    Full Text Available The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.O objetivo deste trabalho foi combinar hibridação somática assimétrica (fusão doador-recipiente ou fusão gama e transferência cromossômica mediada por microprotoplastos como uma ferramenta para o mapeamento cromossômico em Citrus. Microprotoplastos de Swinglea glutinosa foram irradiados com doses de 50, 70, 100 ou 200 raios gama e fusionados com protoplastos de pomelo cv. Ruby Red ou de tangor Murcott. Houve sucesso na formação de colônias de células, e a análise AFLP confirmou a presença de S. glutinosa nos genomas do tangor 'Murcott' e do pomelo 'Ruby Red'.

  10. Herdabilidade da necessidade de calor para a antese e brotação em pessegueiro

    Directory of Open Access Journals (Sweden)

    Citadin Idemir

    2003-01-01

    Full Text Available A herdabilidade da necessidade de calor para antese e brotação em pessegueiro [Prunus persica (L. Batsch] foi estudada em ramos de 16 cultivares e seleções de baixa, média e alta necessidade de calor e 11 progênies oriundas de hibridações entre elas. Os ramos foram submetidos, previamente, a 2 ºC por 500 horas para satisfazer a necessidade de frio. O valor estimado da herdabilidade média para a necessidade de calor em gemas florais foi de 45% e 57%, em 1999 e 2000, respectivamente. Para gemas vegetativas, o valor estimado foi de 30%, em 1999. 'BR-1', 'Barbosa', 'Chula', 'Chinoca' e 'Eldorado' transmitem melhor o caráter necessidade de calor para as progênies do que os demais genótipos estudados. Os registros observados suportam um modelo de herança quantitativa com genes de maior efeito para menor necessidade de calor. A seleção de indivíduos com maior necessidade de calor para floração tende a retardar a floração sem, contudo, retardar com a mesma intensidade a época de brotação.

  11. El carácter fronterizo de las identidades contemporáneas. El caso de Chiapas

    Directory of Open Access Journals (Sweden)

    Moisés Esteban Guitart

    2010-01-01

    Full Text Available La tesis o hipótesis que se sostiene en el artículo es que la puesta en relación de formas explícitas e implícitas de vida compartida en las sociedades contemporáneas conlleva el auge de identidades híbridas, múltiples o complejas. Entendiendo por identidades híbridas la confluencia de múltiples identificaciones en la biografía personal. Siendo la globalización y la pluralización cultural las formas fundamentales de la sociedad contemporánea, la movilidad se convierte en su principal ingrediente. Se describe la presencia de distintos elementos culturales conformadores de identidad en Chiapas. Internet, la emigración a Estados Unidos o el Tratado de Libre Comercio entre México, Estados Unidos y Canadá se hibrida con la heterogeneidad identitaria (religiosa, étnica, lingüística existente en el territorio. Se discute el carácter híbrido de las sociedades contemporáneas y las consecuencias sociales y políticas que conlleva.

  12. Den iscenesatte folkelighed

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    Néstor Garcia Canclini

    1994-09-01

    Full Text Available Hvad er "det folkelige" og hvad er "folkekultur"? Néstor Garcia Canclini har i denne artikel sat sig for at dekonstruere begrebet "det folkelige" og beskrive den måde, forskellige grupper bruger og misbruger begrebet på. Han analyserer tre fremherskende idéer om folkelighed: folkloristernes romantiske idé om det oprindelige, der blev ødelagt af massekulturen, massemediernes popularitetsbegreb og endelig populisternes - både de højre- og venstreorienteredes - iscenesættelse af folkeligheden. Desuden ser han på to marxistiske skolers opfattelse af forholdet mellem de dominerende og undertrykte klasser og forskellige definitioner af folkelig- hed. Ligesom Anamaria Fadul argumenterer Canclini for nødvendigheden af en ny, tværdiciplinær forskning, som opfanger kompleksitet og flerty- digheder i forholdet mellem de undertrykte og dominerende og ser "det folkelige" som en tragisk komedie med modsætninger og flertydighed snarere end en evigt fremadskrivende episk fortælling. Artiklen er en forkortet udgave af kapitel 5 i Canclinis bog fra 1989: "Culturas Hibridas - Estrategias para entrat y salir de la modernidad. Mexico: Grijalbo.

  13. O PAPEL DA ARGUMENTAÇÃO NO ENSINO DE CIÊNCIAS: LIÇÕES DE UM WORKSHOP

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    Daniela Lopes Scarpa

    Full Text Available RESUMO: O objetivo do presente artigo é apresentar e dialogar com os trabalhos produzidos neste número especial, resultado de palestras proferidas no workshopArgumentação no Ensino de Ciências. Os três primeiros artigos discutem mais especificamente a argumentação como prática epistêmica da ciência. O quarto e o quinto propõem elementos orientadores para o planejamento de Ambientes de Aprendizagem argumentativos e, juntamente com os três primeiros, assumem o ensino por investigação/modelagem como abordagem privilegiada para isso. Os três últimos trazem os desafios metodológicos da pesquisa e apresentam perspectivas diferenciadas de análise de situações argumentativas. No diálogo com esses textos, retomo o conceito de hibridação entre cultura escolar e científica, em que a argumentação está na zona de fronteira entre essas culturas; relaciono as etapas da investigação científica com o processo de argumentação e explicito as ações esperadas de professores e alunos na elaboração de atividades argumentativas.

  14. DESIGN OF ALGORITHMS BASED ON BEHAVIOUR FOR THE CONTROL OF MINIBOTS // DISEÑO DE ALGORITMOS BASADOS EN COMPORTAMIENTOS PARA EL CONTROL DE MINIBOTS

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    Maritza Bracho de Rodríguez

    2012-12-01

    Full Text Available Using the Distributed Artificial Intelligence and the Distributed Robotics as a frame of reference, in this work are designed, developed and implemented algorithms to control autonomous, mobile, reactive, rational, proactive and sociable small robots. These minibots are capable to exhibit behaviors inspired in biological societies. Through the development of this work it was found that if the robot has to perform simple tasks, a reactive architecture is more convenient, efficient and effective. While for the performance of tasks of medium or greater complexity, is recommend the use of a hybrid architecture that allows the incorporation of deliberative reasoning.// RESUMEN: Tomando como marco de referencia a la Inteligencia Artificial Distribuida y la Robótica Distribuida, en este trabajo se diseñan, desarrollan e implementan algoritmos para el control de pequeños robots autónomos, móviles, reactivos, racionales, proactivos y sociables. Estos minibots son capaces de exhibir comportamientos inspirados en las sociedades biológicas. En los resultados alcanzados durante el desarrollo de este trabajo se encontró que cuando el robot debe ejecutar tareas simples, la arquitectura reactiva es la más conveniente, eficiente y efectiva, mientras que para la ejecución de tareas de complejidad mediana o mayor, es más recomendable el uso de arquitecturas hibridas que permitan la incorporación de procesos deliberativos.

  15. Regeneração de plantas após fusão de protoplastos de tangelo 'Page' e toranja 'Lau Tau' Plant regeneration after protoplast fusion of 'Page' tangelo and 'Lau Tau' pummelo

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    Dayse Cristina de Carvalho

    2007-08-01

    Full Text Available Buscou-se a hibridação somática entre tangelo 'Page' e toranja 'Lau Tau' visando à produção de porta-enxerto semelhante à laranja-azeda, por esta espécie ser considerada um provável híbrido entre C. reticulata e C. grandis. Após isolamento, fusão e cultivo de protoplastos, obtiveram-se brotações que foram enxertadas in vitro, em laranja 'Hamlin'. Dezessete plantas foram aclimatizadas em casa de vegetação. A análise de citometria de fluxo confirmou a constituição diplóide dessas plantas. Marcadores moleculares RAPD das plantas regeneradas apresentaram padrão de bandas similar ao de tangelo 'Page'. Entretanto, todas as plantas apresentaram conformação fenotípica diferente dos genitores.This work aimed to produce somatic hybrid between 'Page' tangelo and 'Lau Tau' pummelo in an attempt to regenerate a similar rootstock to sour orange, because this species is considered a probable hybrid between C. reticulata and C. grandis. After protoplast isolation, fusion and culture regenerated shoots were in vitro grafted on 'Hamlin' sweet orange. Seventeen plants were acclimatized in a greenhouse. Citometric flow analyses revealed that all plants are diploid. RAPD molecular markers of regenerated plants had the same pattern as compared to 'Page' tangelo. However, all plants had phenotypic traits different from both genitors.

  16. Comunicação e imaginário nos contos do cinema contemporâneo: uma estética em transição

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    Maria Beatriz Furtado Rahde

    2008-09-01

    Full Text Available Este artigo analisa alguns contos do cinema de ficção científica, refletindo sobre hibridações da comunicação visual desse gênero de filme. Estabelece relações entre processos comunicativos dos tradicionais contos de fadas com novas possibilidades tecnológicas pós-modernas em Spielberg e Lucas. Uma nova estética visual se expressa, com modelos humanos fragmentados, cibernéticos, heróis robôs. A aceitação desse gênero pelo espectador confere legitimidade à mudança de estilo, causando, por conseqüência, transições estético/imagísticas. Palavras-chave: Imaginário; cinema; estética; pós-moderno. ABSTRACT This article analyses some tales in science fiction movies, aiming at a reflection on hybridities within visual communication in this kind of movies. It tries to establish relations between communicative processes of traditional fairy tales with new technological post-modern possibilities, in Spielberg and Lucas. A new visual aesthetics is expressed, with fragmented human models, cybernetics, heroic robots. The acceptance of this genre on the part of the spectator grants legitimacy to a change in style, causing therefore, aesthetic/imagistic transitions. Keywords: Imaginary; movie; aesthetic; postmodern.

  17. Experiences with a small scale Solar/Wind pilot installation for basic electrification in the chilean altiplano

    Energy Technology Data Exchange (ETDEWEB)

    Sapiain, Raul; Ovalle, Ricardo; Torres, Ariel; Brockmeyer, Ricarda; Schmidt, Reinhold [Centro de Energias Renovables/Universidad de Tarapaca, Arica, (Chile); Meer, Andreas V. [Solar Institute, Juelich (Germany)

    1997-12-31

    Basic rural electrification programmes are already carried out in the rural areas of northern Chile by local communities and local governments using photovoltaic systems. Solar Home Systems, 12 VDC are installed for individual households while systems for schools, public lighting etc. are realized with bigger systems, 220 VAC. Within a cooperation with the Solar Institute of the Fachhochschule Juelich, Germany, the Renewable Energy Center of the University of Tarapaca designed, installed and evaluated the first solar/wind hybrid installation for basic electrification in northern Chile, realized in Colpitas, a typical small village in the chilean altiplano. The following paper presents results and experiences of this first pilot installation. [Espanol] Ya se estan llevando a cabo programas de electrificacion rural basica en las areas rurales del Norte de Chile por las comunidades y los gobiernos locales, usando sistemas fotovoltaicos. Se instalan Sistemas Domesticos Solares de 12VDC para casas-habitacion individuales, mientras que los sistemas para escuelas, alumbrado publico, etc., se ejecutan con sistemas mas grandes de 220VAC. Con la coperacion del Instituto Solar de la Fachhochschule en Julich, Alemania, el Centro de Energia Renovable de la Universidad de Tarapaca, diseno, instalo y evaluo, la primera instalacion hibrida solar/viento para electrificacion basica en el Norte de Chile, realizado en Colpitas, un pueblo tipico pequeno del altiplano chileno. El siguiete articulo presenta los resultados y experiencias de esta primera instalacion piloto.

  18. Exergoeconomic optimization of tetra-combined trigeneration system

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    Juan Carlos Burbano J.

    2015-01-01

    Full Text Available Se pretende obtener configuraciones óptimas de sistemas de trig eneración para satisfacer demandas de electricidad y cargas tér micas de calentamiento y enfriamiento, evaluando el impacto de los costo s de producción de electricidad, vapor y agua fría. Un sistema de trigeneración produce electricidad, calor y enfriamiento usando esa electrici dad o calor disponible. Se hace énfasis en sistemas que usan re frigeración por absorción, incluyendo uno que usa refrigeración hibrida de abso rción eyectocompresión, llamado sistema tetracombinado de tri generación. La evaluación del desempeño de los sistemas se lleva a cabo med iante el análisis exergético y exergoeconómico de las alternati vas propuestas para determinar la eficiencia exergética y los costos exergétic os de la producción de las utili dades del sistema. Se usó el m étodo de Algoritmos Genéticos para la optimización de las plantas de trigeneración. La optimización muestra beneficios importantes en los costos e xergéticos de los productos por medio de la maximización de la eficiencia exergét ica de los diferentes sistemas.

  19. Atores religiosos populares e midiático-consumismo católico / Popular religious actors and catholic media-consumerism

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    Emerson Sena da Silveira

    2010-01-01

    Full Text Available Propõe-se, com o presente artigo, refletir sobre as implicações da cultura de consumo bem como da mídia sobre os atores religiosos populares, a partir do estudo de uma comunidade católico-carismática de estrato popular. Perpassarão as reflexões questionamentos tais como as mediações entre os atores religiosos, por meio de suas atividades, e a dimensão das forças midiáticas e do consumo. Partindo dessas considerações, constata-se que os fluxos e fronteiras entre mídia, consumo e carismatismo católico põem em pauta novas formas de hibridação entre religião e mundo pós-moderno. / This article aims to reflect upon the implications of the culture of consumerism as well as the media for popular religious actors, starting from the study of a poor Catholic Charismatic community. The reflections will touch questions such as the mediations between the religious actors, through their activities, and the dimension of media forces and consumerism. Starting from these considerations, it is noticed that the flows and boundaries between media, consumerism and Catholic Charismaticism put in debate new forms of hybridization between religion and post-modern world.

  20. Taxa de polinização cruzada em cubiu Natural outcrossing in cocona

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    Waldelice Oliveira de Paiva

    1999-01-01

    Full Text Available Com o objetivo de avaliar a frequência de hibridação natural em Cubiu (Solanum sessiliflorum Dunal foram avaliadas em Manaus, AM, duas cultivares que se diferenciavam pela presença ou ausência de pigmentação de antocianina nos ramos, pecíolos e nervuras das folha. A taxa de polinização cruzada foi avaliada em três épocas do florescimento das plantas e foi estimada em 31%. Durante o desenvolvimento da cultura, observou-se grande presença de abelhas dos gêneros (Apidae Paratrigona, Trigona e Melipona e coleópteros Crysomelidae.In order to evaluate the natural rates of outcrossing in cocona (Solanum sessiliflorum Dunal, two cultivars, one with antocyanin presence and other without antocyanin, were planted in Manaus, AM, Brazil. The percentage of cross-polination was evaluated in three plant flowering periods and was estimated in 31%. During the period of cultivation, high presence of beas of Apidae genus Paratrigona, Trigona and Melipona and beetle Crysomelidae were observed.

  1. Nonhemocyte sources of selected lysosomal enzymes in Biomphalaria glabrata, B. tenagophila and B. straminea (Mollusca: Pulmonata

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    Gary E. Rodrick

    1981-09-01

    Full Text Available The specific activities of acid phosphatase, alkaline phosphatase, β-glucuronidase, lysozymes, glutamate-oxalacetate transaminase and glutamate-pyruvate transaminate were determined in the head-foot and digestive gland of Brazilian Biomphalaria glabrata (Touros, B. tenagophila (Caçapava and B. straminea (Monsenhor Gil. All six enzymes were detected inthe 3000g supernatant. Both cytoplasmic enzymes, glutamate-oxalacetate and glutamate-pyruvate transaminase exhibited the highest specific activities. In the case of the four hydrolytic enzymes assayed, β-glucuronidase exhibited the highest specific activity while lysozyme showed the lowest activity. All six enzymes are thought to be produced by cells within the head-foot and digestive gland of B. glabrata, B. tenagophila and B. straminea.Foram determinadas, na massa cefalopedal e na glândula digestiva de Biomphalaria glabrata de Touros (Rio Grande do Norte B. tenagophila de Cacapava (Sao Paulo e B. straminea de Monsenhor Gil (Piauí, as atividades específicas das seguintes enzimas: fosfatase acida, fosfatase alcalina, beta-glucuronidase, lisozima, transaminase glutâmico-oxalacetica e transaminase glutâmico-piruvica. As seis enzimas referidas foram detectadas no sobrenadante a 3000g. Ambas as enzimas citoplasmaticas - transaminases glutamico-oxalacetica e glutamico-piruvica - mostraram as atividades específicas mais altas. No caso das quatro enzimas hidrolíticas, a beta-glucuronidase revelou a mais alta atividade específica, enquanto a lisozima revelou a mais baixa. E admitido que todas as seis enzimas sao produzidas por celulas presentes na massa cefalopedal e na glândula digestiva das tres especies de moluscos examinadas.

  2. Metaplasia mieloide do baço na Ancilostomose

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    W. O. Cruz

    1934-06-01

    Full Text Available Pesquizamos, no figado e baço de dez casos puros de ancilostomose, elementos hemocitopoieticos; verificamos o peso do baço em 23 casos de individuos com idades compreendidas entre 3 e 60 anos; não encontramos, em nenhum caso, celulas hemoformadoras no figado. Em sete casos, encontramos, no baço, elementos da série vermelha em adiantado estado de evolução (eritroblastos ortocromaticos de nucleo picnotico. Em alguns destes casos observamos megacariocitos e numerosos mielocitos eosinofilos. Os tres casos que não apresentavam metaplasia mieloide no baço, eram os de individuos acima de 50 anos de idade. Entretanto, em outro caso de um individuo com 59 anos esta metaplasia foi verificada. Em individuos acima de 20 anos, o peso médio do baço, em nove casos, mostrou-se igual ao peso normal. Em 14 casos, compreendidos entre 3 e 14 anos, o peso deste orgão foi sempre sensivelmente mais elevado que nos normais de idade correspondente. Estes resultados sugerem a possibilidade de ser a metaplasia mieloide responsavel pelos aumentos de pezo nos baços de individuos jovens, vitimados pela anemia ancilostomica. A notavel proliferação dos eritroblastos ortocromaticos mostra que o grão e a rapidez da regeneração sanguinea, após a administração de ferro, são devidos, essencialmente, á grande quantidade de hemoglobina já preformada no baço e na medula ossea dos organismos ancilostomados.

  3. PROCESO DE INFECCIÓN DE ANTRACNOSIS POR Colletotrichum truncatum EN PAPAYA MARADOL

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    INDIRA ROJO-BÁEZ

    Full Text Available RESUMEN Colletotrichum truncatum es un hongo patógeno causante de antracnosis en fruto de papaya (Carica papaya L. y responsable de pérdidas postcosecha. El objetivo de este trabajo fue inducir el proceso de infección de C. truncatum en frutos de papaya var. Maradol bajo condiciones controladas y decribir el progreso de la enfermedad mediante herramientas histopatológicas y microscopía óptica para elucidar el ciclo de vida del patógeno. La penetración directa de la cutícula por medio de apresorios ocurrió alrededor de las 30 h después de inoculación (hdi; las hifas intramurales crecieron en las paredes celulares de células epidérmicas a las 30-48 hdi; la colonización necrotrófica inició a las 72 hdi; hifas intracelulares crecieron dentro de celulas de parénquima causando una extensiva degradación celular lo cual se asocia a la producción de lesiones típicas de antracnosis. El rompimiendo de la cutícula del hospedero ocurrió entre las 96-148 hdi. El ciclo de vida del patógeno se completó a las 96 hdi con la formación de acérvulos. Las observaciones microscópicas en frutos de papaya revelaron que C. truncatum es un patógeno intramural subcuticular.

  4. [Tropical spastic paraparesis in a non tropical region].

    Science.gov (United States)

    Pias-Peleteiro, L; Pias-Peleteiro, J M; Arias, M

    2015-10-16

    Introduccion. El virus linfotropo humano de celulas T tipo 1 (HTLV-1) es el agente causal de la paraparesia espastica tropical. Su prevalencia, elevada en determinadas areas tropicales, es baja en Europa y Norteamerica. Casos clinicos. Se describen dos casos de paraparesia espastica tropical en varones naturales y residentes en Galicia. Se realizaron estudios analiticos en la sangre y el liquido cefalorraquideo (LCR), examenes neurofisiologicos y resonancia magnetica craneal y medular. En ambos pacientes, la presentacion clinica fue la de una mielopatia cronica, con cuadro torpido y progresivo que evoluciono a paraparesia espastica. Un paciente desarrollo uveitis antes de la clinica neurologica. En los dos casos, el estudio del LCR demostro leve pleocitosis linfoide, ligera hiperproteinorraquia, bandas oligoclonales negativas y anticuerpos anti-HTLV-1 positivos. La reaccion en cadena de la polimerasa para HTLV-1 resulto positiva en ambos casos. La resonancia magnetica raquidea resulto normal en un paciente y mostro en el otro hiperseñal medular dorsal, que desaparecio tras el tratamiento. No se demostraron datos de polineuropatia periferica. Recibieron corticoides e interferon alfa, con leve mejoria y estabilizacion del cuadro clinico. La anamnesis dirigida revelo antecedentes de contactos sexuales de riesgo en regiones endemicas de HTLV-1. Conclusiones. La uveitis asociada a HTLV-1 podria ser predictora de paraparesia espastica tropical. Esta es probablemente una entidad infradiagnosticada (alto porcentaje de portadores asintomaticos, clinica insidiosa y bajo indice de sospecha en areas no endemicas). Debe considerarse su diagnostico en zonas no tropicales que reciben inmigrantes de areas endemicas y tambien en regiones con una tradicional emigracion a regiones tropicales.

  5. [Lymphocytic hypophysitis and hypertrophic pachymeningitis: description of a possible case associated to IgG4 pathologies].

    Science.gov (United States)

    Blanco-Cantó, M Empar; Dávila-González, Pablo; López de Silanes, Carlos; Cuadrado-Pérez, M Luz; Ortega, Gloria; Porta-Etessam, Jesús

    2015-06-01

    Introduccion. Las patologias relacionadas con la infiltracion de linfocitos y celulas inflamatorias se clasifican, segun su topografia, en patologia orbitaria idiopatica, paquimeningitis hipertrofica idiopatica, sindrome de Tolosa-Hunt e hipofisitis linfocitaria. Presentamos a una paciente que comenzo con una hipofisitis linfocitaria hace ocho años y ha presentado varios episodios de paquimeningitis hipertrofica variables en su localizacion. Caso clinico. Mujer de 55 años, en seguimiento por cefalea de dos meses de evolucion, que ingreso por empeoramiento clinico con paralisis del III par derecho completo, lagrimeo y rinorrea. Se realizo una resonancia magnetica cerebral que demostro la presencia de una lesion sellar compatible con hipofisitis linfocitaria. Se inicio tratamiento con corticoides con mejoria inicial, pero, tras dos años, la paciente presento empeoramiento clinico con cefalea y parestesias perioculares derechas. Se repitio la resonancia, donde se observaron varias lesiones extraaxiales, siendo el resto de estudios normales, por lo que se diagnostico paquimeningitis hipertrofica idiopatica y se inicio tratamiento de nuevo con corticoides. En el seguimiento posterior, la paciente presento nuevas lesiones en diferentes localizaciones y mal control algico, por lo que se añadio tratamiento inmunomodulador. Ante la asociacion de hipofisitis linfocitaria y paquimeningitis hipertrofica, se completo el estudio con determinacion de la IgG4 en el suero, que resulto negativa. Conclusiones. La asociacion de hipofisitis linfocitaria y paquimeningitis hipertrofica con las patologias relacionadas con la IgG4 se ha descrito recientemente. A pesar de que en nuestra paciente no se ha confirmado el diagnostico, se debe considerar esta relacion en aquellos casos idiopaticos y, sobre todo, si se asocian otras manifestaciones sistemicas.

  6. [Hypertrophic pachymeningitis secondary to IgG4-related disease: case report and review of the literature].

    Science.gov (United States)

    Rodríguez-Castro, Emilio; Fernández-Lebrero, Aida; López-Dequidt, Iria A; Rodríguez-Osorio, Xiana; López-González, Francisco J; Suárez-Peñaranda, José M; Arias, Manuel

    2015-10-01

    Introduccion. La paquimeningitis hipertrofica es un trastorno infrecuente que produce un engrosamiento focal o difuso de la duramadre. Puede ser idiopatica o secundaria a procesos infecciosos, autoinmunes o neoplasicos. La recientemente descrita 'enfermedad relacionada con IgG4' podria ser la causa de bastantes cuadros considerados criptogenicos. Caso clinico. Mujer de 54 años, con historia de asma bronquial, que consulto por cefalea, vertigo y perdida de audicion por su oido izquierdo. En la resonancia magnetica cerebral con gadolinio se objetivo engrosamiento y realce dural, que se extendia desde la pared lateral del seno cavernoso izquierdo y la parte medial del lobulo temporal al angulo pontocerebeloso y parte del tentorio homolaterales. El liquido cefalorraquideo presentaba 10 leucocitos/µL (90% mononucleares), con 1 g/L de proteinas y sin consumo de glucosa. El estudio anatomopatologico mostro fibrosis y un infiltrado linfoplasmocitario, con 16 celulas plasmaticas IgG4+ por campo de gran aumento. El resto de estudios analiticos y microbiologicos resultaron normales o negativos. La tasa plasmatica de IgG4 estaba dentro de los limites normales. Tratada con esteroides, se produjo mejoria clinica acompañada de la practica desaparicion de las alteraciones detectadas en la neuroimagen. Conclusiones. La paquimeningitis hipertrofica como manifestacion de la enfermedad relacionada con IgG4 puede diagnosticarse basandose en los hallazgos de la resonancia magnetica si la IgG4 plasmatica esta elevada. En casos dudosos, habra que recurrir a la biopsia meningea. La corticoterapia suele ser eficaz y representa la primera linea terapeutica.

  7. [MicroRNAs and their neuroimmunoregulator mechanisms in multiple sclerosis. Development of biomarkers for diagnosis].

    Science.gov (United States)

    Sánchez-Chaparro, M Marisela; Rodríguez-Sánchez, Iram P; Barrera-Saldaña, Hugo A; Martínez-Villarreal, Laura E; Resendez-Pérez, Diana; Gámez-Escobedo, Idalia A

    2015-06-16

    Introduccion. Los microARN (miARN) son moleculas que han generado gran atencion como reguladores de procesos de silenciamiento genico en diferentes organismos. La desregulacion de los mecanismos efectuados por estas moleculas se vincula al desarrollo y progresion de los trastornos relacionados con el sistema inmune. Diferentes estudios exponen que los miARN desempeñan un papel fundamental en procesos neuronales e inmunes, y se relacionan con los mecanismos de las enfermedades que afectan ambos sistemas. La esclerosis multiple (EM) es una enfermedad neurodegenerativa debida a la desmielinizacion axonal causada por procesos autoinmunes. Objetivo. Mostrar la estrecha relacion de las funciones regulatorias de los miARN en vias de señalizacion neuroinmunologicas en el desarrollo de la EM, asi como su estudio como biomarcadores diagnosticos para su uso en pacientes. Desarrollo. En la literatura cientifica se ha estudiado y establecido el papel de los miARN como moduladores de los procesos celulares. Sin embargo, poco se ha abordado sobre su funcionalidad en las celulas gliales dentro de los procesos de plasticidad neuronal, regulacion de la desmielinizacion y reconstitucion axonal, por lo que su revision constituye el proposito de este escrito. Ademas, algunos miARN previamente evaluados se describen para su enfoque diagnostico para la deteccion, curso y tratamiento de la EM, y se encuentran en investigacion o implementacion. Conclusiones. Existe una fuerte evidencia del papel que realizan los miARN en los mecanismos homeostaticos axonales durante la evolucion de la EM. Esto representa un area de estudio para explorar el uso de estas moleculas para la comprension de esta enfermedad, su diagnostico oportuno y la evolucion en los pacientes.

  8. Effects of juvenile hormone analogue on ecdysis prevention induced by precocene in Rhodnius prolixus (Hemiptera: Reduviidae

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    Patricia de Azambuja

    1984-12-01

    Full Text Available Precocene II, added to the meal of fourth-instar larvae of Rhodnius prolixus (25 mug/ml of blood, induced an in crease in the duration of the molting cycle. This effect was related to the decrease of both the nuclear area of the prothoracic gland cells and the mitotic activity in epidermal cellS. juvenile hormone analogue applied topically (60 mug/insect together with Precocene II treatment avoided atrophy of the prothoracic glands and induced a higher number of epidermal mitosis accelerating the time of subsequent ecdysis. A possible relationship between juvenile hormone and production of ecdysone is discussed.Adicionado ao sangue alimentar na dose de 25 mug/ml o precoceno II causou um aumento no período de intermuda em ninfas de 4o. estadio de Rhodnius prolixus. Este atraso da muda foi relacionado com a diminuição da área dos núcleos das celulas das glandulas protoracicas e com a queda da atividade mitotica das células da epiderme do inseto. Um análogo de hormônio juvenil aplicado topicamente (60 mug/inseto junto com o tratamento oral com precoceno II preveniu a atrofia das glândulas protorácicas e induziu um aumento no número de mitoses nas células da epiderme, diminuindo o período de intermuda nestes insetos.A possivel relação entre a ação do hormônio juvenil e a producao de ecdisona pelas glândulas protorácicas e discutida.

  9. Fibroma odontogenico en maxilar inferior. Apropósito de un caso

    Directory of Open Access Journals (Sweden)

    Gustavo Guzmán

    2013-10-01

    Full Text Available Title: Mandibular odontogenic fibroma. A case report.Resumen: Los tumores benignos son neoplasias que se pueden presentar en cualquier parte de la cavidad oral estos pueden ulcerarse, sangrar o interferir con la masticacion o con la adhesion de protesis dentales, tambien presentan un desarrollo lento, una superficie mucosa conservada y compresion de estructuras vecinas. Los fibromas odontogenicos son tumores benigno poco frecuente caracterizado por tejido fibroso con celulas de tejido conectivo, este se puede presentar en cualquier etapa de la vida, no tiene predileccion de sexo y se da mas en la mandibula que en los maxilares. El objetivo de este artículo es describir un caso clínico de este tumor, hacer una breve revisión de la literatura, analizar sus características clínicas e histológicas y la actitud terapéutica a tomar.(DUAZARY 2012 No.2, 190 - 195AbstractBenign tumors are growths that can occur in any part of the oral cavity they can ulcerate, bleed, or interfere with chewing or the adhesion of dental prostheses, also show a slow development, a preserved mucosal surface and compression of adjacent structures. Odontogenic fibroma is a fairly common tumor tissue characterized by fibrous connective tissue cells, this can occur at any stage of life, has no sex predilection an occurs more in the mandible than the maxilla. The aim of this paper is to describe a case of this tumor, a brief review of the literature, analyze their clinical and histological features and therapeutic approach to take.Keywords: Ossifying fibroma; benign neoplasms; fibromas; Jaw Neoplasms.

  10. [Response to everolimus in patients with giant cell astrocytoma associated to tuberous sclerosis complex].

    Science.gov (United States)

    Mateos-González, M Elena; López-Laso, Eduardo; Vicente-Rueda, Josefina; Camino-León, Rafael; Fernández-Ramos, Joaquín A; Baena-Gómez, M Auxiliadora; Peña-Rosa, M José

    2014-12-01

    Introduccion. Los astrocitomas subependimarios de celulas gigantes (SEGA) se presentan en el 5-20% de los pacientes con complejo esclerosis tuberosa (CET) y son los tumores cerebrales mas comunes en el CET. Son tumores benignos, de estirpe glioneural, que se desarrollan fundamentalmente en las primeras dos decadas de la vida, en general cercanos al foramen de Monro, y pueden ocasionar hidrocefalia e hipertension intracraneal. Constituyen la principal causa de muerte en el CET. Recientemente, los inhibidores mTOR han demostrado ser una alternativa terapeutica a la reseccion quirurgica. Objetivo. Describir nuestra experiencia con everolimus para el tratamiento de pacientes con SEGA y CET. Pacientes y metodos. Estudio prospectivo de la respuesta de los pacientes con CET y al menos un SEGA en crecimiento. Resultados. Recibieron tratamiento tres mujeres y tres varones con una edad media de 12,3 años. Un paciente habia sido previamente intervenido quirurgicamente por SEGA con hidrocefalia. El diametro maximo medio del SEGA al inicio del tratamiento era de 15,3 mm (rango: 11,3-24,8 mm). Se inicio tratamiento con everolimus, 2,5 mg/dia por via oral en pacientes con superficie corporal 1,2 m2. Dos pacientes presentaron hipertrigliceridemia; uno, anorexia; otro, un afta; y una paciente, amenorrea. La reduccion media del volumen del SEGA a los tres meses de tratamiento fue del 46%, y la reduccion se mantuvo estable en controles posteriores (6-25 meses). Conclusiones. El tratamiento con everolimus disminuye el tamaño de los SEGA asociados a CET con un perfil de seguridad adecuado, y constituye una alternativa a la cirugia en casos seleccionados.

  11. Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat x maize hybrids

    Directory of Open Access Journals (Sweden)

    Yeshwant R. Mehta

    2000-09-01

    Full Text Available Seven wheat cultivars having some degree of resistance to Bipolaris sorokiniana, Magnaporthe grisea or Xanthomonas campestris pv. undulosa (Xcu provided somaclonal variation for disease resistance. Callus induction varied from 69.4 to 100% across the cultivars, whereas regeneration frequency of R1 plants (regenerated plants of first generation varied between 2.7 and 23.1%. Resistance variation in the R2-regenerated second generation plants was observed for B. sorokiniana and M. grisea but not for Xcu. Attempts were made to fix the resistance characteristics of R3-regenerated third generation somaclones through wheat x maize hybrids. Wheat and maize hybridization of seven wheat somaclones yielded 81 embryos. A total of 11,624 somaclone florets were pollinated, of which 8.4% produced haploid embryos across the seven wheat genotypes. Hybrid embryo production varied between 0 and 25%. The somaclones had a constant chromosome number as observed in their original hexaploid wheat genotypes (2n = 6x = 42 whereas the haploid plants had n = 21. Hybrid embryo production and haploid and dihaploid plant production were affected by 2,4-D concentration, but not by the wheat genotype.Sete cultivares de trigo possuindo certo nível de resistência a Bipolaris sorokiniana, Magnaporthe grisea, Xanthomonas campestris pv. undulosa (Xcu, foram usadas para variação somaclonal, a fim de induzir um nível maior de resistência. A freqüência de indução de calos variou entre 69,4 e 100 entre as cultivares, enquanto que, a freqüência de regeneração de plantas R1 variou entre 2,7 e 23,1, dependendo da cultivar. A variação para resistência entre as plantas de R2 foi observada para B. sorokiniana e M. grisea, mas não para Xcu. Procurou-se fixar as características de resistência dos somaclones (R3 através de hibridação com milho. A hibridação entre trigo e milho produziu 81 embriões zigotos. Um total de 11.624 flores de somaclones foram polinizadas, das

  12. Tesis Doctoral- Síntesis. Estudio sobre la escucha de la voz del locutor con y sin su imagen: análisis del proceso perceptivo y cognitivo del mensaje.

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    Olatz Larrea Estefanía

    2015-11-01

    Full Text Available Este informe presenta una síntesis de la tesis doctoral “Estudio sobre la escucha de la voz del locutor con y sin su imagen” (Larrea, 2015. La  tesis se centra en el estudio comparativo de los procesos perceptivo y cognitivo que experimenta el oyente de radio ante la escucha de una comunicación radiofónica en dos modalidades perceptivas diferenciadas: la voz aislada y la voz acompañada de la imagen de su emisor. Basado en un diseño experimental que hibrida la metodología cuantitativa y la cualitativa, el estudio comprueba empíricamente las diferencias perceptivas y cognitivas del oyente de radio en relación a las sensaciones causadas por la voz, la atribución de efectividad, el proceso de generación de imágenes mentales durante la escucha, la atención prestada al mensaje y su recuerdo, en ambas modalidades de escucha. La investigación ofrece además un amplio recorrido por los procesos psicológicos involucrados en el procesamiento de la información mediada, atendiendo específicamente al tono y el timbre de la voz como principales cualidades de la materia constitutiva de la radio. A continuación se presenta una síntesis de las principales fases del estudio, y una selección de sus resultados y conclusiones.

  13. Eidos y periferia. Rutina y trascendencia in extremis en el horizonte de una humanidad proteica e híbrida

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    César Moreno Márquez

    2012-04-01

    Full Text Available Las interpretaciones ascética y vitalista del eidos son incapaces de pensar a fondo su relevancia fenomenológica. Siempre parece que se valora más su perímetro y cerco, al servicio de la clausura y la consolidación de la unidad (incluso uniformidad: efecto-Procusto, que la diversidad intraeidética, infinita o empíricamente abierta. Más allá de lo típico, las rutinas y asentamientos mundanos (que surten interpretaciones acomodaticias del eidos, la diversidad intraeidética es críticamente eficaz sobre todo en la periferia, al tiempo que propicia la flexibilidad y dilatación interior de cada eidos. Esta problemática es decisiva cuando se trata de pensar la dimensión proteica e hibrida de la cultura humana, así como la posibilidad de diversidad y cordura en un mundo complejo y en expansión.The ascetic and vitalistic interpretations of eidos are unable to think thoroughly its phenomenological relevancy. It always seems that the perimeter and boundary of eidos, at the service of the closure and consolidation of the unity (even uniformity: Procustes effect, is more valued than intraeidetic diversity, infinitely or empirically opened. Beyond the typical, mundane routines and settlements (wich supply accommodating interpretations of eidos, intraeidetic diversity is critically effective especially in the periphery, while enabling the flexibility and inner expansion of each eidos. This problem is crucial when it comes to thinking about the protean and hybrid dimensions of human culture, as well as the possibility of diversity and sanity in a complex and expanding world.

  14. Isolamento e eficiência de plaqueamento de protoplastos de citros

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    COSTA MARIA ANGÉLICA PEREIRA DE CARVALHO

    2002-01-01

    Full Text Available As recentes ferramentas biotecnológicas para o melhoramento in vitro de citros incluem a hibridação somática por fusão de protoplastos. A otimização dos protocolos de isolamento, plaqueamento e cultura de protoplastos, fundamental para maior eficiência na produção de híbridos somáticos, foi avaliada em 11 variedades cítricas. As soluções enzimáticas testadas foram: 1. celulase Onozuka RS, 1%; macerase R-10, 1%, pectoliase Y-23, 0,2%; 2. celulase Onozuka R-10, 0,2 %; macerase R-10, 0,3%; driselase, 0,1%; 3. celulase Onozuka R-10, 1%; macerase R-10, 0,2%; driselase, 0,1%. O plaqueamento dos protoplastos foi realizado em meio de cultura EME 0,7 M, nas densidades de 2 x 10(4; 5 x 10(4; 10(5; 2 x 10(5 e 3 x 10(5 protoplastos.mL-1, no escuro, a 25 ± 1degreesC. A solução enzimática 1 possibilitou melhor rendimento no isolamento de protoplastos para a maioria das variedades, exceto para o limão-'Cravo', onde o melhor rendimento foi obtido na solução enzimática 3, e para as laranjas-doces 'Valência' e 'Succari', que apresentaram maiores rendimentos na solução enzimática 2. A eficiência final de plaqueamento, avaliada aos 90 dias de cultivo, foi superior nas densidades de 10(5 e 2 x 10(5 protoplastos.mL-1, para todas as variedades. O desenvolvimento de embriões somáticos foi observado em todas as variedades, exceto para tangor 'Murcote'.

  15. Comportamento agronômico de progênies F4 de cafeeiros oriundos do cruzamento entre os cultivares Mundo Novo e Catuaí

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    Francisco Carlos Pedro

    2011-06-01

    Full Text Available A espécie Coffea arabica L é cultivada em todas as regiões cafeeiras do Estado de Minas Gerais, com predominância dos cultivares Catuaí e Mundo Novo, por seu vigor vegetativo, alto potencial produtivo e boa qualidade de bebida. Visando a identificar e selecionar progênies de cafeeiro com boas características agronômicas, foi instalado e conduzido um experimento na Fazenda Experimental da EPAMIG, no município de Três Pontas, MG. Foram avaliadas 39 progênies na 4ª geração de autofecundação, após o 2º retrocruzamento entre 'Catuaí' e 'Mundo Novo' (genitor recorrente, desenvolvidas pelo programa de melhoramento genético da Epamig. Os cultivares Catuaí Vermelho MG 99, Rubi MG 1192 e Acaiá Cerrado MG 1474 foram utilizados como testemunhas. Foram analisadas as características: produção de café beneficiado em sc.ha-1 de oito safras (1998/1999 a 2005/2006, vigor vegetativo, percentagem de frutos chochos e classificação quanto à peneira. Ficou evidente que existe variabilidade genética dentro do grupo de progênies estudadas. As maiores produtividades foram encontradas na sétima e oitava colheita. As progênies 1189-9-80-1 e 1189-9-80-3 apresentaram melhor desempenho, em todas as características avaliadas e serão selecionadas para futuros trabalhos de melhoramento genético, sendo lançadas como cultivar ou utilizadas em hibridações.

  16. Inovação, tecnologias de informação e comunicação e processos disruptivos Innovación, tecnologias de información y comunicación y processos disruptivos

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    Maria Jose Vicentini Jorente

    2012-10-01

    Full Text Available A Inovação e seus processos, em especial no campo tecnológico, são de interesse da Ciência da Informação como ciência do humano, pois determinam o estabelecimento de novos hábitos, indicadores socioculturais relevantes para a compreensão da história das culturas. Este artigo reflete sobre as problemáticas: se e como as Tecnologias de Informação e Comunicação tem impactado a vida dos nativos digitais; se o indivíduo comum está preparado para vivenciar criteriosamente o entorno tecnológico; como o sistema vigente poderá preparar gerações futuras de profissionais; e como os adultos que cresceram no século XX, em uma sociedade analógica podem preparar os jovens do século XXI para uma realidade digital, extensivamente diferente da deles. À medida que a sofisticação das tecnologias avança, a sociedade tem que continuamente revisar a maneira como se apropria da informação para se ajustar a essas mudanças. Quanto à relevância das metodologias que conduzem para ações inovadoras disruptivas em realidades hibridas como a brasileira, com nichos de pobreza e prosperidade, pode-se dizer que é em países pobres ou com diferenças sociais tão evidentes como as do Brasil onde se denota a necessidade de mudança de paradigma sócio tecnocultural e de uma ação inovadora.

  17. Operational performance of the Avispa-IIE wind generator in microhybrid systems

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Galarza, Raul; Mejia Neri, Fortino [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    The purpose of this paper is to make a general analysis over the operational performance of the Avispa-IIE wind generator in an Solar-Eolic hybrid installation, at an eco-tourist resort. This work was performed through the monitor of the wind generator and the system itself throughout a year, for the acquisition taking the variables of interest and operational parameters that might allow to characterize and to evaluate the general behavior of the system. Herein are the principals characteristics of the wind generator; its performance curve, the basic configuration of the installation and the control philosophy; Likewise, some technical and human problems which arise during the operation of the system are included, the implementation of improvements in the wind generator and the general results acquired during the time of operation of the wind generator in the cited installations. [Espanol] El proposito de este articulo es el hacer un analisis general del comportamiento operacional del aerogenerador Avispa-IIE en una instalacion hibrida Solar-Eolica en un lugar de veraneo eco-turistico. Este trabajo ha sido llevado a cabo mediante el monitor del aerogenerador y el sistema mismo por espacio de un ano, para la adquisicion de las variables de interes y de los parametros operacionales que pudieran servir para caracterizar y evaluar el comportamiento general del sistema. Aqui se incluyen las caracteristicas principales del aerogenerador; su curva de comportamiento, la configuracion basica de la instalacion y la filosofia del control. De la misma manera se incluyen algunos problemas tecnicos y humanos que se originan durante la operacion del sistema y la puesta en practica de las mejoras del aerogenerador y los resultados generales adquiridos durante el tiempo de operacion del aerogenerador en las instalaciones citadas.

  18. Producción de semilla y cruzamientos entre accesiones de maíz del departamento del Magdalena, Colombia

    Directory of Open Access Journals (Sweden)

    Catherine Pardey Rodríguez

    2015-01-01

    Full Text Available A partir de accesiones de maíz con pasaporte del departamento del Magdalena, Colombia, se inició un programa de cruzamientos bajo las condiciones edafoclimáticas del Centro Experimental Agrícola y Forestal de la Universidad del Magdalena (74° 07' - 74° 12' O y 11° 11' - 11° 15' N ubicado en la ciudad de Santa Marta, Colombia, a 2 m.s.n.m. El objetivo en el trabajo fue obtener líneas endocriadas a partir de 10 accesiones de maíz y formar 45 híbridos F1 directos entre ellas. La producción de las líneas endocriadas sólo fue posible con ocho de las accesiones; la accesión 35 procedente de San Pedro de la Sierra (1397 m.s.n.m. no floreció bajo las condiciones del ensayo y la accesión 91 procedente de Fundación (62 m.s.n.m. floreció, pero no formó semilla. Las accesiones 34 y 90 produjeron poca semilla, a diferencia de la accesión 92, que produjo abundante semilla. En general el desarrollo de las mazorcas producidas por autofecundación fue pobre y con baja formación de grano. La cantidad de semilla en F1 fue diferente entre las cruzas. Las accesiones 3202, 88, 89 y 92 fueron buenos parentales con habilidad para cruzarse y producir semilla hibrida, a diferencia de las accesiones 34, 35 y 91 que mostraron pobre adaptación y escasa habilidad en los cruzamientos. Las condiciones edafoclimáticas del Centro Experimental Agrícola y Forestal de la Universidad del Magdalena no son adecuadas para producir semillas en programas de cruzamientos dirigidos de maíz.

  19. Análise dialélica parcial em gerações avançadas para seleção de populações segregantes de trigo

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    Adérico Júnior Badaró Pimentel

    2013-12-01

    Full Text Available O objetivo deste trabalho foi estimar, em gerações avançadas, os efeitos da capacidade geral e específica de combinação de genitores de trigo, bem como selecionar populações segregantes superiores. Doze genitores e suas 36 populações nas gerações F2 e F3, obtidas em arranjo de dialelo parcial, foram avaliados quanto à produtividade de grãos. Utilizou-se o delineamento em látice 7x7, com duas repetições, mais um tratamento para completar o látice. O efeito de capacidade geral de combinação foi significativo, nas duas gerações. Observou-se alta correlação (0,83 dos efeitos de capacidade geral de combinação entre gerações, mas inexpressiva influência dos efeitos de gerações e de anos na classificação dos cruzamentos. As populações provenientes do cruzamento entre os genitores BRS 254, BRS 264 e IAC 364 (Tucuruí III e os genitores MGS 1 Aliança, VI 98053 e UFVT 1 Pioneiro apresentam maior potencial para obtenção de linhagens superiores, quanto à produtividade de grãos. O uso da análise dialélica parcial em gerações avançadas é promissor para programas de melhoramento de trigo por hibridação.

  20. Poliembrionia e potencial de obtenção de híbridos em citros

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    Walter dos Santos Soares Filho

    2014-12-01

    Full Text Available A ocorrência de poliembrionia, elevada heterozigosidade e longo período pré-reprodutivo são os principais obstáculos à geração de variedades híbridas de citros. Este trabalho teve como objetivo a obtenção de indivíduos com potencial de uso como porta-enxertos através de cruzamentos entre diversas variedades. Observou-se que as hibridações envolvendo a tangerineira 'Sunki da Flórida' (TSKFL como parental feminino possibilitaram a geração de maiores quantidades de híbridos, dando-se o contrário em relação àquelas em que os parentais femininos foram as tangerineiras 'Cleópatra', 'Sunki Maravilha', 'Sunki Tropical' e 'Dancy', assim como o limeiro 'Rugoso da Flórida', ocupando posições intermediárias os limoeiros 'Cravo Santa Cruz' e 'Rugoso CNPMF-001'. Confrontando essas observações com as relativas ao grau de poliembrionia dos parentais femininos utilizados, constatou-se, de modo geral, uma relação inversa entre o grau de poliembrionia e o número de híbridos gerados. Os cruzamentos TSKFL x (limoeiro 'Cravo x Poncirus trifolata, TSKFL x citrange 'Yuma', TSKFL x citrangequat 'Thomasville', TSKFL x Citrus webberi, TSKFL x citrange 'Argentina' e TSKFL x citrange 'Coleman', notadamente os quatro primeiros, permitiram a obtenção de elevadas quantidades de híbridos.

  1. Fluxo gênico recíproco entre cultivares de soja convencional e geneticamente modificada

    Directory of Open Access Journals (Sweden)

    Welison Andrade Pereira

    2012-02-01

    Full Text Available O objetivo deste trabalho foi determinar o fluxo gênico recíproco entre duas cultivares de soja, uma tolerante e outra sensível ao glifosato, além de aplicar estimadores para determinar a taxa de fecundação cruzada na população e o número de sementes híbridas na progênie. O experimento compôs-se de quatro blocos com 40 fileiras de soja, com 20 fileiras de cada cultivar (CD217 e CD219RR. No estádio R8, cinco fileiras, distantes 0, 5, 1, 2, 4 e 8 m da cultivar adjacente, foram colhidas, trilhadas e analisadas quanto à ocorrência de fluxo gênico. Como características marcadoras, foram utilizadas as cores da flor, hipocótilo e pubescência, e a tolerância ao glifosato. As cultivares contrastam quanto às características analisadas, cada uma condicionada por um gene com dois alelos, em interação de dominância completa. Na progênie da cultivar tolerante, a maior taxa de híbridos encontrada foi 0, 27% e, na progênie da cultivar sensível, identificou-se 0, 83%; pela hipótese do efeito diluição, as taxas de hibridação natural populacional seriam 0, 104 e 0, 388%, respectivamente. O fluxo gênico recíproco entre as cultivares CD217 e CD219RR não é o mesmo em ambas as direções. Os estimadores propostos são úteis para determinar a taxa de híbridos em amostras de sementes.

  2. Resistência à podridão parda em pessegueiro

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    Juliano dos Santos

    2012-01-01

    Full Text Available A podridão parda, causada por Monilinia fructicola, é a mais importante doença fúngica do pessegueiro, principalmente em áreas quentes e úmidas, como a região produtora de pêssegos no Sul do Brasil. A resistência genética é a forma mais eficiente de controle da doença, além de reduzir o custo de produção e o impacto ambiental. A cultivar Bolinha, considerada padrão de resistência à doença, produz frutos de baixa qualidade e suas flores não têm a mesma resistência ao fungo. Este trabalho teve como objetivo buscar novas fontes de resistência a esta doença. Além da cultivar Bolinha, foram submetidos à inoculação com suspensão de 1 x 10(5 conídios mL-1 do patógeno, flores de 11 seleções de pessegueiro, frutos de 12 seleções e 20 plantas de cinco "seedlings" oriundos de hibridações. Os resultados indicaram que a seleção Conserva 930 e a cultivar Jubileu foram as que tiveram maior nível de resistência nas flores. Frutos das seleções Conserva 1798, Conserva 1596, Conserva 1218 e Cascata 1493 proporcionaram níveis de resistência semelhantes aos da cultivar Bolinha, e destas, apenas a Conserva 1798 manteve o mesmo desempenho em estádio mais avançado de infecção por M. fructicola.

  3. Tratamiento Superficial de Acero Galvanizado con Películas Híbridas formadas por 3-(trimetoxisililpropil metacrilato (TMSPMA y Tetraetoxisilano (TEOS Surface Treatment of Galvanized Steel with Hybrid Films formed by 3-(trimethoxysilyl methacrylate (TMSPMA and Tetraethoxysilane (TEOS

    Directory of Open Access Journals (Sweden)

    Sandra R Kunst

    2012-01-01

    Full Text Available Acero galvanizado fue revestido con una película híbrida a partir de una solución formada por los precursores silanos 3-(trimetoxisililpropil metacrilato (TMSPMA y tetraetoxisilano (TEOS con adición de cerio. El uso de capas hibridas orgánico-inorgánico representa una alternativa ambientalmente aceptable para mejorar la resistencia de una serie de materiales metálicos frente a los procesos corrosivos. Se emplearon tres tiempos de inmersión (2, 10 y 15 minutos y se caracterizaron las películas mediante microscopia electrónica de barrido, evaluando también el carácter hidrofóbico de las películas. El comportamiento electroquímico de los revestimientos obtenidos fue determinado por el monitoreo del potencial de circuito abierto, polarización potenciodinámica e impedancia electroquímica. Los resultados que se obtuvo evidenciaron el efecto del tiempo de permanencia dentro de la solución en la uniformidad de la película y consecuentemente sobre la resistencia a la corrosión del mismo.Galvanized steel was coated with a hybrid film obtained from a solution formed by silane precursor 3-(trimethoxysilyl methacrylate (TMSPMA and tetraethoxysilane (TEOS with addition of cerium. The use of hybrid organic-inorganic layers represents an environmentally friendly alternative to improve resistance against corrosion of a series of metallic materials. Three immersion times (2, 10 and 15 minutes were used and the films were characterized by scanning electron microscope evaluating film wetability was at the same time. The electrochemical behavior of the coatings obtained was evaluated by open circuit potential, potentiodynamic polarization and electrochemical impedance. The results showed the effect of immersion time in the solution on the film uniformity and consequently on the corrosion resistance of these films.

  4. Teor e composição química do óleo essencial de Hyptis marrubioides Epl., Lamiaceae em função da sazonalidade = Content and chemical composition of Hyptis marrubioides essential oil in function of seasons

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    Suzan Kelly Vilela Bertolucci

    2010-07-01

    Full Text Available Os oleos essenciais sao principios odoriferos armazenados em celulas especiais da planta. O oleo essencial no genero Hyptis e usado como anestesico, antiespasmodico, antiinflamatorio e pode induzir aborto em doses elevadas. O objetivo deste trabalho foi avaliar a influencia da sazonalidade no teor e composicao do oleo essencial de Hyptis marrubioides. O delineamento experimental foi inteiramente casualizado, com quatro tratamentos (inverno, primavera, verao e outono e seis repeticoes, totalizando 60 plantas. O oleo essencial foi extraido por hidrodestilacao, em aparelho de Clevenger. A analise qualitativa do oleo essencial foi por cromatografia em fase gasosa (CG/EM. Na estacao do verao, as folhas das plantas de H. marrubiodes apresentaram os maiores teores de oleo essencial. Nas demais estacoes, os teores de oleo essencial obtidos foram semelhantes entre si. Os componentes majoritarios no oleo essencial foram os monoterpenoides α-tujona e β-tujona. As concentracoes relativas dos picos correspondentes a β-tujona nao apresentaram diferencas consideraveis nas quatro estacoes. Os sesquiterpenoides oxigenados (cedrol e cariofilenol e nao-oxigenados (α-copaeno, β-cariofileno, germacreno D e cadaleno foram encontrados em menores quantidades no oleo essencial, entretanto observam-se diferencas quantitativas ao longo das estacoes.Essential oils are odorous principles stored in special plant cells. The essential oil in the Hyptis genus is traditionally used as an anesthetic, antispasmodic, antiinflammatory, and can induce abortion in high doses. The purpose of this work was to evaluate the influence of seasons in essential oil content and chemical composition of species H. marrubioides. A completely randomized complete design was used with four treatments (winter, spring, summer and autumn and six replications, totaling 60 plants. The essential oil was obtained by hydrodistillation with a Clevenger apparatus. The qualitative analysis of the

  5. In vitro activity of 2-pyridinecarboxylic acid against trypanosomes of the subgenus Schizotrypanum isolated from the bat Phyllostomus hastatus = Atividade in vitro do ácido 2-piridinocarboxílico em tripanossoma do subgênero Schizotrypanum isolado do morcego Phyllostomus hastatus

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    Paulo Roberto Ceridóreo Corrêa

    2011-09-01

    Full Text Available The effect of 2-pyridinecarboxylic acid (picolinic acid on trypanosomes of the subgenus Schizotrypanum isolated from the bat Phyllostomus hastatus was determined in this study. Picolinic acid, at 50 ƒÊg mL-1, inhibited epimastigote growth by 99% after 12 days incubation. In addition, trypomastigote motility decreased by 50% after 6h and completely after 24h in the presence of 50 ƒÊg mL-1 picolinic acid. The 50% cytotoxic concentration on HEp-2 cell line was275 ƒÊg mL-1 after 4 days incubation. Altogether, these results indicate higher toxicity against trypanosomes. The inhibitory effect of picolinic acid on epimastigote growth can be partially reversed by nicotinic acid and L-tryptophan, suggesting a competitive inhibition. Furthermore, two anti-Trypanosoma (Schizotrypanum cruzi drugs were also evaluated with regard to bat trypanosome growth. Benznidazole, at 50 ƒÊg mL-1, inhibited epimastigote growth by 90% after 12 days incubation. Nifurtimox, at the same concentration, caused 96% growth inhibition after four days incubation. Corroborating a previous study, bat trypanosomes are a good model for screening new trypanocidal compounds. Moreover, they can be used to study many biological processes common to human pathogenic trypanosomatids.O efeito do acido 2- piridinocarboxilico (acido picolinico sobre um tripanossoma do subgenero Schizotrypanum isolado do morcego Phyllostomus hastatus foi determinado neste estudo. O acido picolinico, na concentracao de 50 ƒÊg mL-1, inibiu 99% do crescimento de epimastigotas apos 12 dias de incubacao. Alem disso, houve um decrescimo de 50 e 100% na mobilidade dos tripomastigotas apos 6 e 24h, respectivamente, em presenca de acido picolinico na concentracao de 50 ƒÊg mL-1. A concentracao citotoxica 50% para celulas HEp-2 foi de 275 ƒÊg mL-1 apos quatro dias de incubacao. Esses resultados indicam maior toxicidade contra os tripanossomas. O efeito inibitoriodo acido picolinico sobre o crescimento de

  6. Fruit anatomy of species of Solanum sect. Torva (Solanaceae Anatomía del fruto en especies de Solanum sect. Torva (Solanaceae

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    Franco E. Chiarini

    2010-12-01

    Full Text Available The mature fruits of 10 South American species of Solanum sect. Torva were studied. Cross and longitudinal microtome sections, stained with astra blue/basic fuchsin, were made for microscopic examination. All species present an epidermis formed by a unistrate layer of small, isodiametric cells, with dense content and cellulosic walls. Immediately below, a hypodermis is always found, consisting of a well-defined layer of lignified cells with a single calcium oxalate crystal occupying the whole lumen of each cell. This is followed by one layer of cellulosic, isodiametric cells with dense cytoplasm and then several collenchymatous layers, sometimes with sclerified cell walls. The mesocarp comprises two zones histologically differentiated: an external one (formed by regular, vacuolated, medium-sized cells with small intercellular spaces, and an internal one, commonly juicy, and developing proliferations among the seeds. The fruits analyzed are alike, and despite some particularities, they can be classified as berries in the conventional sense. All the traits examined agree with the ornithochorous dispersal syndrome. The homogeneity in fruit traits may be due to shared habit, habitat and sexual system.Se estudiaron los frutos maduros de 10 especies sudamericanas de Solanum sect. Torva. Se examinaron en microscopio cortes microtómicos transversales y longitudinales teñidos con azul astral/fucsina básica. Todas las especies presentaron una epidermis unistrata de células pequeñas, isodiamétricas, de contenido denso y paredes celulósicas. Inmediatamente por debajo se encontró siempre una hipodermis, formada por una capa bien definida de células lignificadas con un cristal de oxalato de calcio en el lúmen de cada célula. A continuación se halló otra capa de celulas isodiamétricas, celulósicas, de contenido denso, y luego varias capas de colénquima, en ocasiones con paredes esclerificadas. El mesocarpo presentó dos zonas histologicamente

  7. Attachment evaluation of embryonic stem cells on a pau-coated non-woven fabric: a potential source for bioartificial assist devices

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    Alejandro Soto Gutiérrez

    2006-01-01

    Full Text Available Las células embrionicas han sido estudiadas recientemente como un recurso atractivo para la construcción de órganos artificiales. La proliferación y subsiguiente diferenciación de las células embrionicas dentro de los mismos dispositivos artificiales deben ser consideras. Recientemente, hemos desarrollado un hígado bioartificial usando una tela sintética con fibras huecas recubiertas con un poliaminouretano, el cual facilita la atracción celular dentro del dispositivo hepático. En esta ocasión hemos evaluado la capacidad de atracción celular de las células embrionicas y de cuerpos embrionicos así como la funcionalidad de hepatocitos derivados de células embrionicas de raton en la fibra sintetica. Para diferenciar un gran numero de hepatocitos derivados de las celulas embrionicas, nosotros formamos cuerpos embrionicos y los expusimos al factor 2 de creciemiento de los fibrobastos y a la forma sintetica del factor de crecimiento de los hepatocitos asi como dimetilsulfoxido y dexametasona. La adherencia celular y morfología sobre la fibra sintetica fue evaluada regularmente usando microscopio electronico (SEM. Para evaluar la diferenciación celular examinamos la expresión del gen de la albúmina. Para probar la función de los hepatocitos derivados in Vitro, nosotros evaluamos la capacidad de metabolizar amonio y lidocaina, encontramos que las células cultivadas sobre la fibra sintética expresan y secretan mayores cantidades de albúmina y metabolizan significativamente mejor que los controles. En este trabajo de investigación hemos demostrado que existe una adecuada atracción de las células embrionicas de ratón cultivadas sobre la fibra sintética y que también son capaces de diferenciarse en hepatocitos funcionales. Por esta razón, el cultivo y subsiguiente diferenciación de las células embrionicas sobre la fibra sintética podría ser útil en el desarrollo de biosoportes para la construcción de hígados bio

  8. Germinomas cerebrais (teratomas atípicos da pineal Cerebral germinomas (atipical teratomas of the pineal region

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    Alexandre Alencar

    1981-12-01

    Full Text Available São estudados três casos de germinomas cerebrais, sendo um tópico, da região pineal, e dois ectópicos, chamados germinomas supra-selares ou quiasmáticos. Estes últimos parecem ser mais agressivos que os seus congeneres tópicos, pois alem de se disseminarem pelas cisternas basais, costumam infiltrar as paredes do III ventrículo, fórnix, hipotálamo, nervos e quiasma ópticos. Caracteristicamente produzem um enorme aumento das proteínas liquóricas, desacompanhado de uma pleocitose proporcional. As proteínas liquóricas aumentadas sao globulinas cujas reações mostram-se fortemente positivas, possivelmente produzidas pelas celulas de aspecto linfocitario que constituem parte desta neoplasia. O aumento das proteinas liquoricas e tao acentuado que em presenca de uma crianca ou adolescente com sindrome de hipertensão intracraniana, hiperproteinoraquia sem pleocitose e sinais clínicos ou radiológicos sugestivos de localização na base do crânio a possibilidade de um germinoma quiasmático deve ser seriamente considerada, pois o quadro e muito sugestivo.Three cases of Cerebral Germinomas, one of them situated in the pineal region (topical and two ectopical of the so called suprasellar or chiasmal Germinomas are studied. These last ones may be more aggressive than the congeneral topical ones, since besides it's dissemination throughout the basal cisternas they usually infilstrate into de walls of the III Ventricle, Fornix, Hypothalamus, Chiasma and Optic nervs. A high level of proteins in the spinal fluid without proportional increase of the cells is characteristic. Globulins are the predominant fraction of the increased spinal fluid proteins, as indicated by the highly positive laboratory test. These globulins are possibly produced by the lymphocytes which are part of this neoplasm. The high level of protein in the spinal fluid without proportional increase of the cells, in the presence of intracranial syndrome in a child or

  9. Flow prediction and heat transfer in a cooling square duch of a gas turbine blade using CFD; Predicciones de flujo y transferencia de calor en un conductor de alabe enfriado de turbina de gas utilizando CFD

    Energy Technology Data Exchange (ETDEWEB)

    Urquiza B, Gustavo [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico); Sierra E, Fernando [Universidad Nacional Autonoma de Mexico, Mexico D.F. (Mexico); Kubiak S, Janusz; Campos A, Rafael [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2003-10-15

    A numerical investigation has been conducted to study the turbulent flow and the heat transfer in a blade turbine coolant passage involving a 180 degrees turn. The study provides a comparison test for two turbulence models. The numerical solution was conducted employing two models for turbulence, the renormalization Group Theory (RNG) and the Reynolds Stress Model (RSM), using a refined grid to model with detail the recirculating flow. Computations are performed with a commercial finite volume code which solves three dimensional, incompressible, Navier-Stokes and Energy equations. At the passage turn, significant secondary flows are present, formed by a pair of symmetrical vortices. Results have been compared against published experimental data for Re = 53 000. Very good agreement is achieved for the prediction of the local static pressure distribution along the passage. A strong effect of rotation has been observed mainly in the flow field as described in detail in the paper. [Spanish] En este trabajo se presenta una investigacion numerica para estudiar el flujo turbulento y la transferencia de calor en un conducto de enfriamiento de un alabes de turbina de gas con giro de 180 grados. El estudio proporciona una comparacion de resultados para dos modelos de turbulencia. La solucion numerica emplea dos modelos de turbulencia: el modelo de esfuerzos de Reynolds (RSM) y el modelo de la teoria del grupo de renormalizacion (RNG), utilizando una malla refinada para modelar con detalle el flujo de recirculacion. Los calculos fueron realizados con un codigo comercial de volumenes finitos el cual resuelve las ecuaciones tridimensionales de Navier-Stoke y de energia para flujo incompresible. En la seccion de giro del conductor, aparecen flujos secundarios significativos, formados por un par de celulas simetricas. Los resultados han sido comparados contra datos experimentales de la literatura para Re = 53 000. se obtuvo un buen acuerdo para la prediccion de la distribucion de

  10. Atividade de três drogas antivirais sobre os herpesvírus bovino tipos 1, 2 e 5 em cultivo celular Activity of three antiviral drugs against bovine herpesviruses 1, 2 and 5 in cell culture

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    Renata Dezengrini

    2010-10-01

    Full Text Available A atividade de três fármacos antivirais (Aciclovir [ACV], Ganciclovir [GCV] e Foscarnet [PFA] foi testada in vitro frente aos herpesvírus bovino tipos 1 (BoHV-1, 2 (BoHV-2 e 5 (BoHV-5. Para isso, utilizou-se o teste de reducao de placas virais em cultivo celular, testando-se diferentes concentracoes dos farmacos frente a 100 doses infectantes para 50% dos cultivos celulares (DICC50 dos respectivos virus. Pelo teste de MTT (3-(4,5-Dimethylthiazol- 2-yl-2,5-diphenyltetrazolium bromide, verificou-se que concentracoes inferiores a 200ƒÊg/mL dos tres antivirais resultaram em indices de viabilidade de celulas MDBK e Hep2 superiores a 80%. Com base na concentracao citotoxica para 50% das celulas (CC50 e na concentracao dos farmacos efetiva para inibir em 50% o numero de placas virais (EC50, calculou-se o indice de seletividade (IS dos antivirais para os tres herpesvirus. Assim, o ACV demonstrou ser moderadamente ativo frente ao BoHV-1 (EC50: 112,9ƒÊg/mL e IS: 4,5, ao BoHV-2 (EC50: 114,2 ƒÊg/mL e IS: 4,5 e BoHV-5 (EC50: 96,9ƒÊg/mL e IS: 5,3. O GCV apresentou atividade moderada frente ao BoHV-2 (EC50: 33,5ƒÊg/mL e IS: 16,6 e, em menor grau, contra o BoHV-5 (EC50: 123,2ƒÊg/mL e IS: 4,5, sendo ineficaz frente ao BoHV-1 (EC50: 335,8ƒÊg/mL e IS: 1,7. O PFA apresentou atividade antiviral mais pronunciada, sendo o unico farmaco que, na concentracao de 100ƒÊg/mL, inibiu completamente a producao de placas pelos tres virus testados. O PFA foi o mais efetivo in vitro frente ao BoHV-1 (EC50: 29,5ƒÊg/mL e IS: 42,2, ao BoHV-2 (EC50: 45,2ƒÊg/mL e IS: 27,6 e ao BoHV-5 (EC50: 7,8ƒÊg/mL e IS: 160,6. Portanto, os resultados obtidos indicam que o PFA pode se constituir em um candidato para terapia experimental de infeccoes pelos herpesvirus de bovinos in vivo.The activity of three anti-herpetic drugs (Acyclovir [ACV], Gancyclovir [GCV] and Foscarnet [PFA] was tested against bovine herpesvirus 1 (BoHV-1, 2 (BoHV-2 and 5 (BoHV-5 in vitro using the

  11. Evaluación tomográfica y radiográfica de la obturación radicular tridimensional de conductos radiculares únicos tratados con tres técnicas de obturación radicular: Estudio in Vitro

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    Mónica Alexandra Pavón Granja

    2016-09-01

    Full Text Available El principal reto durante la terapia endodóntica es la obturación radicular, considerada como fase crítica ya que de esta va a depender el éxito o el fracaso. Por varios años se ha investigado tanto materiales como técnicas que permitan el sellado hermético tridimensional del sistema de conductos radiculares, biocompatibilidad que permita la adecuada reparación de los tejidos periapicales y que la obturación se mantenga con el tiempo proporcionando salud. Objetivo: Evaluar la adaptación tridimensional de tres técnicas de obturación radicular definitiva a nivel del tercio apical de conductos radiculares únicos. Materiales y métodos: Estudio de tipo experimental y aleatorizado, la muestra fue de 42 raíces de dientes uniradiculares divididos en 3 grupos de estudio (n=14, previamente se realizó un registro radiográfico periapical digital de toda la muestra posteriormente fueron instrumentados con técnica hibrida, desinfectados con NaOCl al 5,25% + activación ultrasónica y EDTA al 17%, GA fue sometido a técnica de obturación lateral en frío, GB aplicado técnica de obturación termomecánica McSpadden y GC se obturó con técnica termoplastificada (BeFill-VDW. Resultados: Los grupos C y B observados a través de tomografía computarizada presentaron mejores resultados de obturación radicular que el grupo A que no presentó un buen sellado apical, sin embargo, en el grupo C se observó mejores resultados que en A y B Conclusiones: Las técnicas de obturación radicular definitivas con la técnica termoplastificada mostraron ser ideales para proporcionar un sellado apical hermético durante el tratamiento endodóntico en la fase de obturación

  12. Amor e conjugalidade na contemporaneidade: uma revisão de literatura Amor y conyugalidad en la contemporaneidad: una revisión literaria Contemporary love and conjugality: a literature review

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    Leandro Castro Oltramari

    2009-12-01

    Full Text Available O presente artigo se propõe a realizar uma revisão bibliográfica sobre a temática do amor e da conjugalidade no mundo contemporâneo trazendo contribuições de autores como Bozon, Gagnon e outros. O amor tem sido uma temática de discussão diversificada das ciências humanas e sociais e principalmente da Psicologia. A perspectiva adotada neste trabalho apresenta as contribuições da sociologia da sexualidade em uma revisão que traz o amor como uma perspectiva de interação social vivenciada como sentimento amoroso. Esta análise centra-se principalmente na teoria dos roteiros sexuais, compreendendo o amor como uma prática social, mas vivenciada como sentimento. Esta forma híbrida constitui uma das principais características do amor na contemporaneidade.La propuesta del presente artículo es realizar una revisión bibliográfica sobre el tema del amor y la conyugalidad en el mundo contemporáneo, trayendo contribuciones de autores como Bozon, Gagnon entre otros. El amor ha sido un tema de discusión de las ciencias humanas, sociales y principalmente de la psicología. La perspectiva adoptada en este trabajo trae contribuciones de la sociología de la sexualidad. Esta revisión coloca el amor como una perspectiva de interacción social vivenciada como sentimiento amoroso. Este análisis se centra principalmente en la teoría de los scripts sexuales, comprendiendo el amor como una práctica social vivenciada como sentimiento. Esta forma hibrida constituye una de las principales características del amor en la contemporaneidad.The purpose of this article is to provide a bibliographic review about the theme of love and conjugality in the contemporary world including contributions from authors such as Bozon, Gagnon and others. The theme of love has been discussed not only in human and social science but particularly in psychology. The perspective adopted in this work presents contribution from the sociology of human sexuality. Its revision

  13. Caracterização molecular de uma progênie de tangerineira 'Clementina Fina' e 'Montenegrina' Molecular characterization of a progeny between tangerines 'Clementina Fina' and 'Montenegrina'

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    Roberto Luis Weiler

    2010-07-01

    Full Text Available Os citros apresentam uma taxonomia muito complexa, principalmente com relação ao número de espécies que constituem o gênero Citrus e os gêneros afins. Genótipos classificados como espécies podem ter sido originados por hibridação interespecífica e preservados por meio da embrionia nucelar. O presente trabalho teve como objetivo caracterizar uma população de tangerineiras híbridas oriundas do cruzamento das tangerineiras 'Clementina Fina' (Citrus clementina Hort. ex Tan., genitor feminino, e 'Montenegrina' (Citrus deliciosa Ten., genitor masculino, utilizando marcadores do tipo microssatélites (SSR. Com 12 pares de primers, foi possível diferenciar 93 acessos do estudo e agrupar a F1 em indivíduos mais próximos do genitor feminino e do genitor masculino. O PIC (Conteúdo de Informação de Polimorfismo dos primers variou de 0,27 a 0,65. Toda a progênie do cruzamento entre 'Clementina Fina' e 'Montenegrina' analisada neste estudo é híbrida, e os SSRs foram eficientes para identificar híbridos com maior similaridade genética em relação aos genitores, mostrando a existência de variabilidade genética entre as plantas da população estudada.Citrus have a very complex taxonomy, especially considering the number of species included in genus Citrus and related genera. What is classified as a species may have originated by interespecific hybridization and preserved through nucellar embryony. This research aimed to characterize a population of hybrid tangerines, originated from the cross of 'Clementina Fina' (Citrus clementina Hort. ex Tan. as female and 'Montenegrina' (Citrus deliciosa Ten. as male parents, using microsatellite molecular makers. With 12 pairs of primers it was possible to differentiate 93 of the studied accessions and to group F1 individuals nearer to the male or to the female parent. The primers PIC (Polymorphism Information Content ranged from 0.27 to 0.65. All the analyzed progeny between 'Clementina Fina

  14. Resistência de genótipos de caupi ao caruncho

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    BARRETO PAULO DIÓGENES

    2000-01-01

    Full Text Available A utilização de resistência genética ao ataque de Callosobruchus maculatus (Fabr. tem sido alvo de investigação científica, especialmente no que diz respeito à identificação de fontes de resistência. O presente trabalho objetivou incorporar, ao grupo de caracteres desejáveis para o cultivo de caupi (Vigna unguiculata (L. Walp., resistência genética ao caruncho (C. maculatus. Foram realizadas hibridações dos genótipos IT81D-1045 e IT81D-1064 (portadores de resistência ao inseto com CNCx 252-1E/FB, CNCx 187-22D-1 e BR 1-Poty (capazes de transferir resistência a viroses, tolerância à seca, formação de grãos com padrão comercial, elevado potencial de produção e adaptabilidade a diferentes condições ambientais. Populações segregantes obtidas destes cruzamentos foram conduzidas pelo método SPD (descendência de uma única vagem, e na geração F5 foram realizadas seleções individuais. As linhagens obtidas foram avaliadas em conjunto com materiais de origens diferentes, utilizando-se parâmetros associados à infestação da praga. Foi constatado que os genótipos avaliados apresentaram variabilidade quanto à preferência à postura, número de insetos emergidos e número de sementes danificadas; as linhas EVx 37-15E e EVx 37-2E foram as que sofreram menor dano causado pelo caruncho; as variáveis número de ovos, número de insetos emergidos e número de sementes danificadas mostraram-se positiva e significativamente correlacionadas entre si; o grupo das linhagens que descendem de genitores resistentes apresenta valores significativamente inferiores aos obtidos pelas demais, o que indica que a resistência ao inseto se transmite geneticamente.

  15. Identidades Desafiadas: Individualización, Managerialismo y Trabajo Docente en el Chile Actual Challenged Identities: Individualization, Managerialism, and Teaching Work in Chile Today

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    Vicente Sisto

    2012-11-01

    Full Text Available Los cambios en el trabajo se han ligado a profundas mutaciones en las identidades sociales, las que se encaminarían a una individualización extrema. Sin embargo, esto no ocurre de modo lineal. Este artículo da cuenta de la transformación del trabajo docente en el contexto de la implementación en Chile de la política de evaluación e incentivos al desempeño. Esta instala nuevos referentes basados en competencia técnica, rendición de cuentas, estándares, resultados y emprendimiento individual, interpelando las identidades de los docentes. Se analiza cómo estos se posicionan frente a la implementación de dicha política. Se realizaron entrevistas a una muestra intencionada de 60 profesores de las regiones Metropolitana y de Valparaíso, las que fueron analizadas mediante análisis discursivo de narrativas identitarias. La principal narrativa utilizada por los docentes da cuenta de una política subjetiva y equivocada, ante la cual oponen una versión del trabajo docente que hibrida elementos de las identidades construidas históricamente por la profesión de docentes con otras narrativas propias de las nuevas generaciones. Esto muestra que las transformaciones identitarias que están ocurriendo operan de un modo heterogéneo.The current labor transformations have been linked to profound changes in social identities, which are thought to be moving towards extreme individualization. However, these changes are not linear. This article reports on the transformation of teaching work in the context of the implementation in Chile of a performance evaluation and incentives policy. This policy is installing new reference points based on technical competence, accountability, standards, results and individual entrepreneurship, thus questioning teachers' identities. The signification that they give to the implementation of this policy is analyzed. Interviews were conducted with a purposive sample of 60 teachers of the Metropolitan and Valparaiso

  16. State of the art of technologies thermosolar to concentration; Estado del arte de las tecnologias termosolares a concentracion

    Energy Technology Data Exchange (ETDEWEB)

    Ramos Berume, Carlos; Huacuz Villamar, Jorge M; Medrano Vaca, M. Consolacion [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

    2000-07-01

    Nowadays Research and Development activities on Solar Thermal Power Generating Systems are supported by some countries around the world. Because of their thermal nature each of these plants can be operated only with solar energy or can be hybridized (solar/fossil fuel) and also with thermal storage systems. These types of solar thermal power plants are: Central Receiver, a circular field of tracking mirrors called helisotats reflects the solar energy onto receiver mounted on top of a central tower. Parabolic Trough, use linear parabolic concentrators to reflect sunlight to a receiver along the focal line (metallic pipe). Inside the pipe solar energy is absorbed in a working fluid with temperatures up to 390 Celsius degrees, which is then piped to a central location to power a conventional steam turbine and Parabolic Dish uses a parabolic dish concentrator to focus solar energy to a receiver and a heat engine/generator, located at the focus of the dish, to generate electrical power. In this document, we present the current status of technologies. A description of market opportunities, cost and technological perspectives are also included. [Spanish] Actualmente la Investigacion and Desarrollo para la generacion de potencia electrica mediante el aprovechamiento de la energia solar por la ruta termodinamica esta siendo apoyada por varios paises. Debido a su naturaleza termica estas pueden operar bajo esquemas de operacion solo con energia solar o en forma de plantas hibridas (solar-combustible fosil) y en algunos casos adaptadas con sistemas de almacenamiento termico. Los tipos de plantas solares de generacion electrica son: Receptor Central (RC), utilizan un campo colector con dispositivos llamados heliostatos arreglados en un campo circular y reflejando la radiacion solar sobre un receptor montado en la parte mas alta de una torre. Canal Parabolica (CP), estas plantas utilizan espejos curvados en forma de una parabola que redirigen los rayos del sol sobre su linea

  17. Melhoramento do cafeeiro: XXIX - Produtividade de cafeeiros de porte reduzido Coffee breeding: XXIX - Productivity of dwarf coffee cultivars

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    C.S. Novaes Antunes

    1964-01-01

    Full Text Available Os cultivares de porte pequeno mais promissores da coleção de cafeeiros da Seção de Genética foram plantados em um ensaio comparativo, instalado em 1954, em Campinas. Os dados de sete anos de colheitas dêsse ensaio revelaram que a progênie de prefixo C 1039-48 do cultivar 'São Bernardo' foi a de melhor produção, e a de prefixo C 1034-4 'San Ramon', a de produção mais reduzida. Outras observações foram efetuadas atinentes à altura das plantas, resistência à geada, tipos de sementes produzidas e tamanho das sementes do tipo chato, analisadas pela peneira média, a fim de melhor caracterizar êsses cultivares. Os resultados colhidos dão informações úteis para o trabalho de melhoramento, indicando plantas e progênies a serem utilizadas principalmente nas hibridações com outros cultivares selecionados.The increasing cost of labor is leading the breeders to select coffee strains with low height which give high yield per area and facilitate the harvesting operation. The present paper refers to a trial established to compare the yielding ability of twelve dwarf coffee progenies of 'Caturra Amarelo', 'Caturra Vermelho', 'San Ramon', 'San Ramon x Bourbon', 'San Ramon x Maragogipe' and 'São Bernardo'. After seven consecutive harvests the total weight of coffee cherries was analysed. It was found that only the progeny 'São Bernardo' - C 1039-48 had a significant higher yield than the tester 'Caturra Vermelho' - C 477-8. The two 'Caturra Amarelo' progenies were in the same group of C 1039-48. Small variation was noticed in connection with bean size and percentages of flat, peaberry and elephant beans. The progenies of cultivar 'São Bernardo' had the largest beans and the highest percentages of normal flat beans. Concerning frost resistance some variation was observed in the susceptibility of the progenies to low temperatures. Only the progeny C 1036-36 revealed to be resistant. The best plants selected in this trial will be used in

  18. Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil Surto autóctone de leishmaniose visceral canina no Estado de Santa Catarina

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    Mário Steindel

    2013-04-01

    Full Text Available The present study reports the first outbreak of autochthonous canine visceral leishmaniasis in Florianópolis, Santa Catarina, southern Brazil. Following the report of two cases of CVL, the Control Center of Zoonotic Diseases conducted a serological survey by ELISA and IFAT assays in seven districts of the Santa Catarina Island. Eleven seropositive dogs of autochthonous transmission were used in the present study. Infection by Leishmania sp. was confirmed by parasitological examination of bone marrow, liver, spleen and lymph nodes, culture in Schneider's medium and PCR. Leishmania sp. isolates were characterized by PCR-RFLP and hybridization with specific probes, allowing for the identification of Leishmania infantum. Autochthonous transmission of this disease in an area with high tourist traffic presents a major public health concern and signifies the emergence of an important zoonosis in southern Brazil. Therefore, the implementation of surveillance and control measures is imperative to prevent the spread of the disease among the canine population as well as transmission to the human population.O presente estudo relata o primeiro surto autóctone de leishmaniose visceral canina (LCV em Florianópolis, Santa Catarina, Brasil. Durante levantamento soro-epidemiológico realizado pelo Centro de Controle de Doenças Zoonóticas (CCZ envolvendo 2.124 cães, 29 (1,37% foram soropositivos para VL (ELISA + RIFI. Onze cães positivos por transmissão autóctone foram utilizados no presente estudo. A confirmação da infecção por Leishmania sp. foi realizada pelo exame parasitológico da medula óssea, fígado, baço e linfonodos, cultura em meio Schneider e PCR. Os isolados de Leishmania sp. foram caracterizados por PCR-RFLP e hibridação com sondas específicas, permitindo a identificação de Leishmania infantum. A transmissão autóctone da LCV em uma área com grande fluxo turístico como Florianópolis representa um preocupante risco à saúde p

  19. Desempenho de clones de copa de seringueira resistentes ao mal-das-folhas Performance of rubber tree crown clones resistant to South American leaf blight

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    Vicente Haroldo de Figueiredo Moraes

    2008-11-01

    Full Text Available O objetivo deste trabalho foi avaliar o desempenho de 18 clones de seringueira resistentes ao Microcyclus ulei, usados como copas enxertadas. Foram utilizados oito clones híbridos de Hevea pauciflora x Hevea guianensis var. marginata, oito de H. pauciflora x H. rigidifolia e dois clones de H. pauciflora, enxertados sobre o painel de CNS AM 7905 - seleção primária de H. brasiliensis - e cultivados em Latossolo Amarelo distrófico, em Manaus, AM. Foram avaliados: perímetro do tronco, estado nutricional e produtividade de borracha seca. Copas enxertadas de híbridos H. pauciflora x H. guianensis var. marginata causam crescimento mais rápido do tronco, com redução do período de imaturidade da seringueira. O alto nível de resistência dos híbridos de H. rigidifolia ao percevejo-de-renda (Leptopharsa heveae justifica a introdução de outros genótipos dessa espécie para novas hibridações. Os clones CPAA C 01, 06, 13, 15, 16 e 45 apresentam excelente potencial de produção de borracha seca, nas condições climáticas e de solos de terra firme da Amazônia Tropical Úmida.The objective of this study was to evaluate the performance of 18 rubber tree clones, resistant to Microcyclus ulei and used as budded crowns. Eight hybrid clones of Hevea pauciflora x Hevea guianensis var. marginata, eight of H. pauciflora x H. rigidifolia, and two clones of H. pauciflora were used as budded crowns onto CNS AM 7905 - a primary selection of H. brasiliensis - and cultivated in a Xanthic Ferralsol, in Manaus, Amazônia, Brazil. Trunk girth, nutritional status and rubber productivity were evaluated. The budded crowns of hybrids of H. pauciflora x H. guianensis var. marginata cause fastest trunk girth increment, with shortening of the imaturity period of rubber tree. The high resistance level of H. rigidifolia hybrids to Leptopharsa heveae justifies the introduction of other genotypes of this species, for new hybridizations. The clones CPAA C 01, 06, 13

  20. Características do cultivar iarana de coffea arabica The iarana cultivar of coffea arabica

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    A. Carvalho

    1975-01-01

    Full Text Available Misturas mecânicas de sementes de cafeeiros selecionados portadores de alelos individuais SH1, SH2 , SH3 , SH4 responsáveis pela resistência de Coffea arabica a grupos de raças fisiológicas de Hemileia vastatrix foram distribuídas aos lavradores com as denominações de cultivar iarana-C 73 e iarana-C 74. As sementes foram misturadas nas proporções, em peso, de 2:12:3:1 e 2:8:2:1, para os genotipos SH1SH1, SH2SH2 , SH3,SH3, e SH4SH4 , para os anos de 1973 e 1974, respectivamente. Todos os cafeeiros devem ser portadores dos alelos SH5SH5. A mistura foi distribuida em 1973, a 987 lavradores e, em 1974, a 463 lavradores de todas as regiões cafeeiras do Brasil. O iarana deverá ser plantado isoladamente do resto do cafezal em cada propriedade, para observação e seleção das plantas mais adaptadas para produção de sementes. As hibridações naturais que ocorrem entre plantas de diferentes genotipos darão origem a novas combinações genéticas portadoras de vários dos fatores genéticos, com espectro mais amplo de resistência às raças fisiológicas de H. vastatrix.Mechanical mixtures of seeds of selected plants of Coffea arabica carrying the dominant alleles SH1, SH2, SH3, and SH4 conditioning resistance to different races of Hemileia vastatrix were released to the growers as Iarana C 73 and Iarana C 74 cultivars. The seeds were mixed in the proportion of 2:12:3:1 and 2:8:2:1 for the genotypes SH1SH1, SH2SH2, SH2SH3 and SH4SH4 for 1973 and 1974 crops, respectively. The mixed seeds were distributed to 987 farmers in 1973 and 463 coffee farmers in 1974 from most of the coffee regions in Brazil. Iarana is to be planted in isolated plots for observation and selection of the more adapted plants for seed production. Natural hybridization between plants of different genotypes will give rise to new combinations with broader spectrum of resistance to physiological races of H. vastratrix.

  1. Soybean parent selection based on genetic diversity

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    Valéria Carpentieri-Pípolo

    2000-01-01

    Full Text Available Thirty-four soybean lines were assessed for twelve traits. The genetic distances were estimates using multivariate techniques, to identify parents to be included in breeding programs for hybridization. Grouping by the Tocher method, from generalized Mahalanobis distances, divided the 34 lines into four groups. The most important agronomic traits, weight of seeds per plot, plant height, height of first pod and days to maturity were considered when recommending for crossing. The following crosses were recommended based on the genetic divergence and the key agronomic traits: lines 23, 10, 2, 27 and 25 (group I with genotype 6 (group II and genotype 16 (group III. Thus only ten crosses would be made, representing only 2% of the total crosses which could be made in the partial diallel among the 34 lines assessed, which would allow up to 561 combinations.Trinta e quatro linhagens de soja foram avaliadas para doze características. As distâncias genéticas foram estimadas utilizando técnicas multivariadas com objetivo de identificar parentais a serem incluidos em um programa de melhoramento envolvendo hibridação. O agrupamento pelo método de Tocher, a partir das distâncias generalizadas de Mahalanobis, dividiu as 34 linhagens em 4 grupos. As caracterísiticas agronômicas mais importantes, peso de sementes por parcela, altura de planta, altura da primeira vagem e dias para maturação foram consideradas para a recomendação dos cruzamentos. Os seguintes cruzamentos foram recomendados baseado na divergência genética e nas características agronômicas chave: linhagens 23, 10, 2, 27 e 25 (grupo I com genótipo 6 (grupoII e com o genótipo 16 (grupo III. Portanto somente 10 cruzamentos poderiam ser realizados representando somente 2% do total de cruzamentos qu poderiam ser realizados em um dialelo parcial entre as 34 linhagens avaliadas as quais admitiriam até 561 combinações.

  2. Avaliação da superfície relativa do sistema radicular de cafeeiros Root surface area of coffee plants by the titration method

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    Luis Carlos da Silva Ramos

    1980-01-01

    Full Text Available Avaliou-se a superfície relativa do sistema radicular de cafeeiros dos cultivares Catuaí e Arábica de Coffea arabica, do 'Guarini' de C. canephora e do 'Icatu', descendente de hibridações entre essas espécies, pelo método titulométrico ligeiramente modificado. Outras medidas foram tomadas para a caracterização dos cultivares: peso seco e comprimento das raízes e da parte aérea e diâmetro do caule. O cultivar Guarini, além de apresentar maior superfície relativa, tem, também, maior peso seco de raízes, da parte aérea, maior diâmetro do caule e maior comprimento da parte aérea. Observaram-se altas correlações entre os dados da superfície relativa, peso seco das raízes e peso da parte aérea. Sugere-se que o método da titulação seja aplicável em investigações sobre o estudo do sistema radicular em geral.The root surface areas of 10 months old coffee plants (Coffea arabica L. cvs. Arabica and Catuaí, C. canephora Pierre cv. Guarini, and C. arabica x C. canephora cv. Icatu were evaluated by an adaptation of the WILD & VOIGHT (8 titration method. The root and shoot dry weight, stem diameter, shoot and root lengths were also measured. The plants were assigned in a randomized complete-block design with 5 blocks and 4 replications per plot. They were grown in polyethylene bags in a coffee nursery under 50% sun light. A greater root surface area, dry weight, stem diameter and shoot length was found in the cultivars Guarini. A high correlation between root surface area and root and shoot dry weights was observed for all studied coffee cultivars. The use of the titration method is suggested for measuring effects of physical, chemical and biological agents on root systems of coffee plants as well as other species.

  3. Determinação de metodologia para oisolamento de protoplastos de tangerina Cleópatra (Citrus reshni Hort. Methodology choice for protoplast isolation in Cleopatra mandarin (Citrus reshni Hort.

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    R.P. de Oliveira

    1995-04-01

    Full Text Available A hibridação somática via fusão de protoplastos vem sendo utilizada no melhoramento de porta-enxertos de citros em diversos países. Nos Estados Unidos, vários estudos demonstram a eficiência de procedimentos no isolamento e cultivo de protoplastos dessa frutífera. O presente trabalho foi realizado com o objetivo de avaliar o efeito do meio de cultivo de calos embriogênicos do porta-enxerto tangerina Cleópatra (Citrus reshni Hort. sobre o isolamento de protoplastos, bem como sugerir alterações de procedimento. Os resultados mostram a possibilidade do isolamento de 1.4 x 10(6 a 4.7 x 10(6 protoplastos por grama de calos da espécie estudada. Verificou-se que, o subcultivo dos calos de tangerina Cleópatra em meio de cultura, sem reguladores 1 hora, sob condições de escuro a 120 rpm, proporcionou maior eficiência de isolamento de protoplastos (4.7 x 10(6 protoplastos/g de calo.Somatic hybridization has been used for citrus rootstock breeding in many countries. In USA, many reports had proved the efficiency of procedures for the isolation and culture of citrus protoplasts. This research was conducted to evaluate the efficiency of procedures of protoplast isolation using embryogenic callus of the Cleópatra mandarin rootstock. Alterations were proposed to increase protoplast isolation and culture method. Results show the possibility of a protoplast yield of 1.4 to 4.7 x 10(6 pps/g.f.w. for Cleópatra tangerine rootstock callus. Protoplast yield can be raised to 4.7 x 10(6 pps/g.f.w. if the embryogenic callus are grown in a medium supplemented only with 4% sucrose and pre-treated with 1% w/v macerozyme for 1 hour, at 120 rpm, in dark, is applied before protoplast isolation.

  4. Cytogenetic characterization of Aloysia virgata Ruiz and Pavan (Verbenaceae

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    Aline Dias Brandão

    2009-08-01

    Full Text Available Since previous cytogenetic reports of Aloysia have only described the meiotic behavior and chromosomal number of some species, the aim of this work was to provide detailed cytogenetic description of Aloysia virgata that would contribute to the understanding of the taxonomical organization of the Verbenaceae. Aloysia virgata had a karyotype with 2n = 36 metacentric chromosomes, all with similar size. The large amount of heterochromatin seen after Giemsa staining was confirmed by C-banding. Four nucleolar organizer regions (NORs were detected with an rDNA 45S probe in two homologous pairs and two sites of 5S rDNA located on one chromosomal pair were detected by fluorescence in situ hybridization. The interphase nucleus was classified as semi-reticulate. Meiotic analysis showed a normal chromosomal behavior, with 18 bivalents in some parts of prophase I and in metaphase I. The number of chromosomes, NORs and 5S rDNA segments did not exclude a possible polyploid origin.O gênero Aloysia reúne aproximadamente 30 espécies, porém sua circunscrição tem sido motivo de controvérsia. São poucos os estudos citogenéticos para o gênero, relatando apenas o número e o comportamento cromossômico. O presente trabalho teve como objetivo, identificar os caracteres citogenéticos de Aloysia virgata da flora brasileira que possam ser utilizados para um melhor entendimento e caracterização dos aspectos citogenéticos da família Verbenaceae, que possam contribuir para a organização taxonômica do grupo. Aloysia virgata apresentou cariótipo com 2n = 36 pequenos cromossomos, todos com o mesmo tamanho. Grande quantidade de heterocromatina foi observada com Giemsa e confirmada pela técnica de banda C. Foram detectadas quatro regiões organizadoras de nucléolo (NORs e dois sítios de rDNA 5S pela técnica de hibridação in situ fluorescente (FISH. O núcleo interfásico foi classificado como semireticulado. Foi realizada a caracterização meiótica, na

  5. Elevada incidência de anormalidades cromossômicas numéricas detectadas por FISH multicentromérico em pacientes com mieloma múltiplo High incidence of chromosomal numerical abnormalities by multicentromeric FISH in multiple myeloma patients

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    Maria de Lourdes L. F. Chauffaille

    2007-02-01

    Full Text Available Este estudo objetivou detectar as alterações genéticas em pacientes com mieloma múltiplo (MM, usando o método de hibridação in situ por fluorescência em interfases (FISH interfásico. Para detectar as alterações numéricas foram usadas sondas multicentroméricas e para os rearranjos mais freqüentemente observados na doença foram utilizadas as sondas lócus específicas para IGH, P53, ciclina D1 e RB1. Foram estudados 34 pacientes com MM em estágio avançado, ainda que recém-diagnosticados, 97% dos quais apresentaram anormalidades numéricas detectadas por FISH, sendo 75% hiperdiplóides, 18% hipodiplóides e 3% tri/tetraplóides. Em relação às demais anormalidades, a deleção 13q foi encontrada em 30% dos casos e o rearranjo IGH, em 25%. Agrupando os pacientes com hipodiploidia e com deleção 13q14 (grupo desfavorável e comparando-os com os demais (grupo não-desfavorável, houve tendência a pacientes jovens no grupo desfavorável (p = 0,06 e níveis de hemoglobina (Hb significativamente mais baixos (This study aimed to characterize genetic alterations by interphase multicentromeric FISH focusing on chromosomal numerical abnormalities and using some locus specific probes for the most frequent aberrations found in the disease, in a homogeneous cohort of 34 advanced stage, but recently diagnosed MM patients; 97% had numerical chromosomal abnormalities detected by FISH, being 75% hyperdiploid, 18% hypodiploid and 3% tri/tetraploid. Using locus specific probes, we found 13q deletion in 30% and IGH rearrangement in 25% of cases. Grouping hypodiploid patients together with del13q (unfavorable group and comparing them to the remaining cases (non unfavorable group we found a trend towards younger patients presenting more unfavorable abnormalities (p = 0.06 and significant lower hemoglobin level (Hb < 8.5 mg/dl, p = 0.03.

  6. Epistemology And The Problem Of Cultural Hybridity In Muhammad Iqbal’s Thought

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    Hawasi Hawasi

    2015-12-01

    hasil dari paradigma positivistik  tersebut  yang  kehilangan  kesakralannya  sebagai  ilmu.  Oleh  karena  itu  paradigma  ini mendapatkan  serangan  filosofis  tidak  hanya  dari  pemikir  Barat  pascamodernisme  namun  juga  dari pemikir  pribumi  yang  pernah  mengalami  penjajahan  dan  imperialisme  Barat.  Salah  satu  pemikir Muslim yang mengritik hegemoni pemikiran Barat terhadap kebudayaan dan peradaban Islam adalah Muhammad Iqbal. Bagi Iqbal, selama beberapa abad lamanya pemikir Muslim tidak mampu berfikir secara  Qurani  akibat  cara  pandang  mereka  yang  melihat  Islam  dari  kacamata  filsafat  Yunani  yang cenderung hanya bersifat rasional-spekulatif. Pemikiran Yunani telah menghegemoni pemikiran Islam begitu  lama  sehingga  perlu  didekonstruksi.  Dalam  perspektif  pasca  kolonial  (postcolonial,  usaha Iqbal  untuk  mendekolonisasi  epistemologi  rasional-spekulatif  Barat  sejak  Plato  hingga  Descartes, menghadapi sikap yang ambivalen. Pengalaman penjajahan Inggris di India yang pernah dialami Iqbal membentuk kultur hibrida dalam proyeknya merekonstruksi pemikiran Islam dalam kaitannya dengan pemikiran Barat. Dengan demikian, Iqbal mencoba menjembatani jurang antara pemikiran rasional- spekulatif Barat dengan kecenderungan mistik-religius pemikiran Islam sebagai upaya mencari “Ruang Ketiga” melalui mimikri sebagai konsekuensi dari interaksi budaya antara penjajah dan terjajah. Kata-Kata Kunci: dekolonisasi epistemologi, budaya hibrida, epistemologi Islam, Ruang Ketiga.

  7. Growth of Pediococcus acidilactici on sugar cane blackstrap molasses

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    Ernani S. Sant’Anna

    1998-09-01

    Full Text Available Pediococcus acidilactici (IL01 has grown in MRS (Man, Rogosa and Sharpe broth modified by substitution of glucose by 2.0% (MRS-2, 3.0% (MRS-3, 4.0% (MRS-4 and 5.0% (MRS-5 sugar cane blackstrap molasses. The highest acid production was obtained in MRS-5 broth maintained at a constant pH of 5.0. The highest biomass production was obtained when P. acidilactici was grown in MRS-5 broth at initial pH 6.5, while productivity was higher in MRS-2 broth (28.16%. When the MRS-2 broth was utilized at initial pH 6.5 for a 20-hour fermentation period, the highest growth rate (dx/dt was found in a period of 8 to 16 hours (0.290 g cells/L.h, while the specific growth rate (µ was 0.175 (h-1 for that period, differently from the 0.441 (h-1 obtained for the period comprising the 4th to the 12th hour. The growth in MRS broth was 5.08% (2.95 g/l higher than in MRS-2 broth (2.80 g/l. The data obtained have shown that P. acidilactici has had a significant growth in molasses as the main carbon source, and that it is possible to substitute MRS glucose by this carbon source with the purpose of obtaining a more economical growth medium for the potential large scale productions.Pediococcus acidilactici (IL01 cresceu em caldo MRS (Man, Rogosa and Sharpe modificado por adição de 2,0% (MRS-2, 3,0% (MRS-3, 4,0% (MRS-4 and 5,0% (MRS-5 de melaço de cana de açúcar, em substituição à glicose. A maior produção de ácido ocorreu em caldo MRS-5 com pH constante 5,0. A produção de biomassa foi mais acentuada em caldo MRS-5 com pH inicial de 6,5, embora a produtividade tenha sido maior em caldo MRS-2 (28,16%. Em caldo MRS-2 e em pH inicial de 6,5 durante uma fermentação de 20 horas, a velocidade de crescimento (dx/dt foi maior entre a 8ª e 16ª hora (0,290 g celulas/L.h enquanto a velocidade específica de crescimento µ foi 0,175 (h-1 para este período, diferente de 0,441 (h-1 obtido no período compreendido entre a 4ª e 12ª hora. O crescimento em caldo MRS foi 5

  8. DOI: 10.4025/actascibiolsci.v33i3.8011 Acta Scientiarum. Biological Sciences Maringá, v. 33, n. 3, p. 239-246, 2011 Acute inflammatory response in Nile tilapia fed probiotic Lactobacillus plantarum in the diet = Resposta inflamatória aguda em tilápia do Nilo alimentada com probiótico, Lactobacillus plantarum na dieta

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    Geovana Dotta

    2011-07-01

    alimentacao foi injetado carragenina ou salina. Apos 6h, realizou-se a coleta de exsudato e sangue para determinacao do hematocrito, contagens totais de eritrocitos, leucocitos, contagem diferencial de leucocitos e atividade fagocitaria no sangue. A suplementacao com probiotico na racao nao influenciou o numero total de eritrocitos, o hematocrito e os numeros de linfocitos e basofilos no sangue dos animais. O numero de neutrofilos foi maior nos peixes suplementados com probiotico einjetados com carragenina. A glicose nos peixes suplementados com probiotico nao-injetados foi maior do que nos injetados com salina. A suplementacao com probiotico potencializou a migracao de celulas para o foco inflamatorio nos injetados com o flogogeno carragenina. Empeixes injetados com salina e carragenina, ocorreu maior atividade fagocitaria no sangue em relacao aos demais tratamentos.

  9. Anatomia e ultra-estrutura foliar de Cyperus maritimus Poir. (Cyperaceae: estratégias adaptativas ao ambiente de dunas litorâneas Leaf anatomy and ultrastructure of Cyperus maritimus Poir. (Cyperaceae: adaptive strategies for coastal dune environment

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    Shirley Martins

    2008-06-01

    Full Text Available Foram analisados aspectos anatômicos e ultra-estruturais foliares de Cyperus maritimus Poir. visando identificar caracteres adaptativos ao ambiente de dunas litorâneas. Para isto, indivíduos ocorrentes nas dunas da Praia de Pipa, Rio Grande do Norte, Brasil, tiveram as folhas submetidas a diferentes análises: microscopia de luz, eletrônica de varredura e de transmissão. Em vista frontal, a epiderme possui tricomas unicelulares, corpos de sílica, cutícula estriada e depósitos de cera. Em secção transversal, a epiderme é unisseriada com parede periclinal externa espessa. Os estômatos são paracíticos, podendo o ostíolo estar obstruído por cera. Abaixo da face adaxial observa-se o parênquima aqüífero. Os feixes vasculares ocorrem em múltiplas fileiras, sendo as periféricas compostas por feixes de diâmetro menor que os feixes da fileira central. Foram observados caracteres relacionados à síndrome Kranz do tipo clorociperóide. Aspectos ultra-estruturais, como cloroplastos presentes na bainha do feixe, esses sem grana evidentes, entre outras características, reforçam a possível ocorrência da via C4 nesta espécie. É a primeira citação para o gênero Cyperus de cloroplasto nas celulas do tecido vascular. Neste trabalho foi possível observar vários caracteres importantes para plantas submetidas a estresse hídrico e salino, como: cera, células epidérmicas com parede periclinal externa espessada, parênquima aqüífero e síndrome Kranz.Anatomical and ultrastructural aspects of Cyperus maritimus Poir. were analyzed with the aim of identifying adaptive features for the dune environment. Collections were made at Pipa beach, Rio Grande do Norte, Brazil. Leaves were submitted to different analyses: light microscopy, scanning and transmission electron microscopy. In frontal view, the epidermis has unicellular trichomes, silica bodies, a striate cuticle and wax deposits. In transverse section, the epidermis is uniseriate

  10. Propriedades do pólen e do estigma de Ocimum basilicum L. (cultivar Maria Bonita para aumentar a eficiência de cruzamentos em programas de melhoramento Ocimum basilicum L. (cultivar Maria Bonita pollen and stigma properties to improve the efficiency of crossings in plant breeding programs

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    A.C Brito

    2010-06-01

    Full Text Available Ocimum basilicum, cv. Maria Bonita (Lamiaceae, conhecido como manjericão, é espécie que apresenta propriedades aromáticas, condimentares e medicinais, por ser rico emóleos essenciais. É muito usado nas indústrias farmacêuticas e de alimentos em geral. O objetivo deste trabalho foi estudar as propriedades do pólen e estigma do manjericão (cultivar Maria Bonita identificando procedimentos simples que possam contribuir para programas de melhoramento. Para análise da disponibilidade, viabilidade do pólen e receptividade de estigma, botões florais foram coletados de hora em hora ao longo do dia, e lâminas eram montadas e coradas, para observação em microscópio óptico. Foi verificado que o manjericão apresenta antese diurna, assincrônica e com a maioria das flores se abrindo entre 10:00 e 11:00 horas. Quanto ao estudo do pólen foi verificado que a viabilidade manteve-se elevada ao longo do dia e a conservação por até 90 dias demonstrou bons níveis de viabilidade. O estigma apresentou receptividade desde a pré-antese até a pós-antese. Estas informações são relevantes para os melhoristas que desejam fazer seleção de genótipos ou hibridações em programas de melhoramento, contribuindo para aumentar o potencial da espécie que já se destaca como produtora de óleos essenciais.Known as basil, Ocimum basilicum cv. Maria Bonita (Lamiaceae is a species that presents aromatic, condimental and medicinal properties, since it is rich in essential oils. This species is largely used in pharmaceutical and food industries. The aim of this work was to study basil (cultivar Maria Bonita pollen and stigma properties, identifying simple procedures that can contribute to plant breeding programs. To analyze pollen availability and viability, besides stigma receptivity, flower buds were collected at every hour throughout the day, and slides were mounted, stained and observed under an optical microscope. Basil presented diurnal

  11. Desempenho de clones de seringueira da série IAC 300 selecionados para a região noroeste do Estado de São Paulo Performance of Hevea clones from IAC 300 series selected in the northwestern region of São Paulo State, Brazil

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    Paulo de Souza Gonçalves

    2001-04-01

    Full Text Available É apresentado o desempenho de 19 novos clones de seringueira [Hevea brasiliensis (Willd. exAdr. de Juss. Muell. Arg.], resultantes de hibridações conduzidas no Instituto Agronômico e avaliados em experimento de pequena escala, tendo o clone RRIM 600 como testemunha. O experimento em campo obedeceu ao delineamento em blocos ao acaso, com três repetições. Com relação à produção de borracha seca, o clone IAC 40 apresentou a maior média (62,22 g/árvore/sangria nos três anos de avaliação, seguido pelo IAC 301 (57,67 g/árvore/sangria e pelo IAC 300 (50,61 g/árvore/sangria, com produções 154%, 138% e 123% superiores em relação ao RRIM 600 (41,04 g/árvore/sangria. Todos os clones selecionados foram vigorosos, com perímetro do caule na abertura do painel variando de 37,81 cm (IAC 317 a 50,90 cm (IAC 315. A porcentagem de plantas aptas a sangria variou de 20,0% (IAC 317 a 100% (IAC 315. Todos os clones apresentaram baixas incidências de quebra pelo vento e de secamento do painel. Não foi detectada nenhuma doença foliar em caráter epidêmico. Dos clones estudados, 15 apresentaram alta resistência à antracnose do painel, e foram superiores ao RRIM 600; os outros cinco apresentaram resistência moderada semelhante ao RRIM 600.The present paper shows the performance of 19 rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss. Muell. Arg.] originated from Hevea breeding programme conducted at the Instituto Agronômico de Campinas (IAC and evaluated in a small scale trial in the northwestern of São Paulo State, Brazil. The old popular clone RRIM 600 of Malaysian origin was used as control. The trial was laid out in a randomized block design with three replications. Concerned to yielding the clone IAC 40 recorded highest yield (62.22 g/tree/tap over three years of tapping which was followed by IAC 301 (57.67 g/tree/tap and IAC 300 (50.61 g/tree/tap yielding 154%, 138% and 123% superior to the control clone and RRIM 600 whereas

  12. Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion Análise transcricional dos genes TIMP-1 e NM23-H1 na invasão celular em astrocitoma difuso e glioblastoma multiforme

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    José Augusto Nasser

    2006-09-01

    determinar se TIMP-1 e NM23-H1 estavam relacinados à inibição da invasão tumoral nas neoplasias do sistema nervoso central, quantificando os níveis de RNA dos respectivos genes extraídos diretamente dos tumores. 10 mg por amostra de RNA total foram fracionados de gel de formaldeído e transferidos para os filmes de hibridação. Níveis específicos de RNAs foram determinados na espectrofotometria. Valores das razões entre NM23-H1/GAPDH e TIMP-1/GAPDH foram submetidos à análise de variabilidade das médias. RESULTADOS: A análise da expressão do gene TIMP-1 mostrou supressão em tumores gliais malignos. CONCLUSÃO: Os resultados indicam que existe relação direta entre níveis baixos de TIMP-1 e malignidade dos gliomas. O gene NM23-H1 foi detectado em todas as amostras, mas não foi possível relacionar sua subexpressão ou superexpressão com algum fenótipo de invasividade.

  13. Divergência genética entre progênies de maracujazeiro- amarelo com base em características das plântulas Genetic divergence among yellow passion fruit progenies based on seed traits

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    Jacson Rondinelli da Silva Negreiros

    2008-03-01

    Full Text Available O objetivo do presente trabalho foi avaliar a diversidade genética entre 24 populações de maracujazeiro-amarelo, discriminando os caracteres mais importantes na avaliação da divergência genética, com base em características das plântulas. Foram coletadas sementes de frutos obtidos a partir de polinização natural de vinte e quatro populações segregantes de meios-irmãos de maracujazeiro-amarelo. Utilizou-se delineamento experimental inteiramente casualizado, em vinte e quatro tratamentos (populações segregantes, com quatro repetições, considerando-se como unidade experimental cada grupo de 50 sementes. Aos 28 dias, avaliaram-se a porcentagem de germinação e o índice de velocidade de emergência (IVE. Aos 45 dias, avaliaram-se porcentagem de sobrevivência, altura das plântulas, comprimento de raiz, número de folhas e massa da matéria seca total das plântulas. Os dados obtidos foram submetidos à análise de variância, e as médias foram agrupadas pelo método de Scott & Knott. A diversidade genética foi estudada de acordo com o método de agrupamento de Tocher, baseado na distância de Mahalanobis (D² e variáveis canônicas. As características que mais contribuíram para a divergência genética foram porcentagem de germinação, número de folhas e IVE. A população 20 pode ser recomendada para hibridação com as outras populações devido à sua alta divergência e também altas taxas de germinação e vigor de sementes.The genetic diversity was studied among passion fruit (Passiflora edulis f. flavicarpa progenies, discriminating the characters most important of seed traits for genetic divergence evaluation. Seeds were extracted of fruits gotten by natural pollination of twenty four half-sib segregant populations. The experiment was outlined as an entirely randomized design with twenty four treatments (segregant populations, four replications and 50 seeds per experimental unit. The germination percentage and the

  14. Modelado de Materiales Compuestos por Elementos Finitos usando Restricciones Cinemáticas Finite Element Modeling of Composite Materials using Kinematic Constraints

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    Oscar E. Ruiz

    2009-12-01

    Full Text Available El propósito de este artículo es presentar simulaciones del comportamiento de materiales compuestos basado en restricciones cinemáticas entre las mismas fibras y entre las fibras y la resina circundante. En la revisión de literatura, los autores han encontrado que las restricciones cinemáticas no han sido plenamente explotadas para modelar materiales compuestos, probablemente debido a su alto costo computacional. El propósito de este articulo es exponer la implementación y resultados de tal modelo, usando Análisis por Elementos Finitos de restricciones geométricas prescritas a los nodos de la resina y las fibras. Las descripciones analíticas del comportamiento de materiales compuestos raramente aparecen. Muchas aproximaciones para describir materiales compuestos en capas son basadas en la teoría de funciones C1Z y C0 Z, tal como la Teoría Clásica de Capas (CLT. Estas teorías de funciones contienen significativas simplificaciones del material, especialmente para compuestos tejidos. Una aproximación hibrida para modelar materiales compuestos con Elementos Finitos (FEA fue desarrollada por Sidhu y Averill y adaptada por Li y Sherwood para materiales compuestos tejidos con polipropileno de vidrio.The purpose of this article is to present simulations of the behavior of composite materials based on kinematic restrictions among the fibers themselves and among fibers and the surrounding resine. In the literature review the authors have found that the kinematic restrictions have not been fully exploited for modeling composite materials, probably due to their high computational expense. The purpose of this article is to show the implementation and results of such a model, by using a Finite Element Analysis of geometric restrictions prescribed to the resine and fiber nodes. Closed analytic descriptions on behavior of layered composite materials are very rare. Many approaches to describe layered composite material are based on the theory of

  15. Potencial de obtenção de novos porta-enxertos em cruzamentos envolvendo limoeiro 'Cravo', laranjeira 'Azeda', tangerineira 'Sunki' e híbridos de Poncirus Trifoliata Potential of obtaining new rootstocks in crosses among 'Rangpur' lime, 'Sour' orange, 'Sunki' mandarin and Poncirus Trifoliata

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    Walter dos Santos Soares Filho

    2008-03-01

    Full Text Available O limoeiro 'Cravo' e a laranjeira 'Azeda' são importantes porta-enxertos de citros. Com o objetivo de explorar seu potencial genético, distintas seleções de limoeiro 'Cravo', empregadas como parentais femininos, foram cruzadas com seleções de laranjeira 'Azeda', tangerineira 'Sunki Maravilha' e híbridos de Poncirus trifoliata. Semelhantemente, seleções de 'Azeda' foram hibridadas com seleções de 'Cravo' e híbridos de P. trifoliata. Avaliaram-se a taxa de vingamento de frutos, a porcentagem de híbridos no conjunto total de indivíduos obtidos (zigóticos e nucelares, a altura e o diâmetro do caule destes, em torno dos 12 meses de idade. O cruzamento entre limoeiro 'Cravo', seleções comum e 'Santa Cruz', com tangerineira 'Sunki 'Maravilha' resultou nas maiores taxas de vingamento de frutos (média de 87,5% e maior percentual de híbridos (média de 64,0%, sendo estes relativamente vigorosos, segundo comparações com seedlings nucelares desse limoeiro. Quanto às hibridações envolvendo a laranjeira 'Azeda', mostraram-se promissoras 'Azeda Jacarandá' x (tangerineira 'Cleópatra' x P. trifoliata seleção 'Swingle 245', pela relativamente elevada taxa de vingamento de frutos (50%, boa porcentagem de híbridos (32,3% e possibilidade de obtenção de híbridos vigorosos, e laranjeira 'Azeda' comum x limoeiro 'Cravo' seleções comum e 'Santa Cruz', pela possibilidade de obtenção de híbridos vigorosos e em quantidades relativamente altas, em torno de 40% de plantas híbridas.'Rangpur' lime and 'Sour' orange are important citrus rootstocks. Aiming to explore their genetic potential, several 'Rangpur' lime selections, as female parents, were crossed with 'Sour' orange selections, 'Sunki Maravilha' mandarin and Poncirus trifoliata hybrids. In the same way, 'Sour' orange selections were crossed with 'Rangpur' lime selections and P. trifoliata hybrids. Parameters evaluated were: fruit set, percentage of hybrids out of the total number

  16. VIABILIDADE DO PÓLEN EM VARIEDADES DE LARANJA DOCE POLLEN VIABILITY IN SWEET ORANGE VARIETIES

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    Edson Tobias Domingues

    1999-01-01

    Full Text Available Uma vez que a polinização é um dos pontos decisivos para o crescimento e desenvolvimento do fruto, contribuindo com os gametas masculinos para a fecundação e determinando, na maioria das vezes, a fixação dos frutos em citros, torna-se necessário conhecer o grau de esterilidade masculina nas diferentes variedades de laranja doce para sua possível utilização em programas de melhoramento. A esterilidade é limitante para programas que envolvam a hibridação sexual, por outro lado possui sua importância econômica em citros induzindo menor número de sementes por fruto em certas variedades. Com a finalidade de caracterizar 44 variedades de laranja doce (Citrus sinensis [L.] osbeck quanto à viabilidade do pólen, foram coletadas anteras das variedades enxertadas sobre tangerineira Cleópatra. As variedades estudadas pertencem aos principais grupos de laranja doce: com acidez (como a laranja 'Pêra', de baixa acidez (como a laranja 'Lima', com umbigo (como a laranja 'Bahia' e sangüíneas (como a laranja 'Rubi Blood'. O percentual de pólen viável foi avaliado por meio da coloração com carmim acético a 25% e contagem sob microscópio ótico. Foram observados valores que variaram desde 12,0% para a 'Pêra Sem Sementes' até 88,8% para a variedade 'Hamlin Reserva'. Os clones de laranja 'Hamlin' mostraram maior percentual de pólen viável. Não foi observada presença de pólen para as variedades produtoras de laranjas de umbigo, originadas da variedade Bahia. As variedades 'Pêra', 'Valência' e 'Natal', as quais são as principais cultivares da citricultura paulista e nacional, apresentaram baixos percentuais de pólen viável.Pollination is one of the most critical points in fruit growth and development, contributing with male gametes for fertilization and determine, greatly, fruit setting in citrus. It is necessary to evaluate male sterility in sweet orange varieties for their possible use in breeding programs. The sterility limits

  17. Diversidade e similaridade genéticas em clones de pimenta-do-reino Genetic diversity and similarity in clones of black pepper

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    José M.D. Gaia

    2005-06-01

    Full Text Available Setenta e oito clones de pimenta-do-reino (Piper nigrum L. foram analisados por meio de eletroforese de isozimas para os sistemas ACP, GOT, SKDH, ACO, G6PDH, PGI, 6PGDH e FUM, para avaliar a diversidade por meio da porcentagem de locos polimórficos, número médio de alelos por locos e heterozigosidade média. A similaridade genética foi obtida por meio do coeficiente de semelhança simples e foi resumida num fenograma de média de grupo. Foram detectados 14 locos e 35 alelos. Os locos que apresentaram maior diversidade foram: G6pdh-1, Acp-1 e Skdh-1. A porcentagem de locos polimórficos variou de 3,57% a 64,29%; o número médio de alelos variou de 0,04 a 1,64; e a heterozigosidade média variou de 0,036 a 0,321. Os baixos valores observados no intervalo de variação da heterozigosidade média são consistentes com a estreita base genética dos genótipos e a amplitude deste intervalo pode estar relacionada com o grau de hibridação (artificial ou natural de cada clone. A similaridade genética variou de 65% a 100%, sendo que 70 clones estiveram contidos na faixa de 85% e 100%, o que ratifica o estreitamento da base genética da espécie e conseqüente homogeneidade dos genomas cultivados.Seventy eight black pepper clones (Piper nigrum L. were analyzed by means of isozyme electrophoresis for the systems ACP, GOT, SKDH, ACO, G6PDH, PGI, 6PGDH and FUM, in order to evaluate the diversity through the percentage of polymorphic loci, mean number of alleles per locus and mean heterozigosity. Genetic similarity was calculated by simple matching coefficient and summarized in the group average phenogram. Fourteen loci and 35 alleles were detected. The loci that presented larger diversity were G6pdh-1, Acp-1 and Skdh-1. The percentage of polymorphic loci ranged from 3.57% to 64.29%; the mean number of alleles ranged from 0.04 to 1.64 and; the mean heterozigosity varied from 0.036 to 0.321. The lower values observed in the interval of variation of the

  18. HETEROSE EM ARROZ IRRIGADO HETEROSIS IN IRRIGATED RICE

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    Elcio Perpétuo Guimarães

    2007-09-01

    Full Text Available

    Com o objetivo de medir a magnitude da heterose, heterobeltiose e heterose padrão para oito características em híbridos de arroz (Oryza sativa L. irrigado, foram avaliados 19 híbridos resultantes de cruzamentos entre duas linhagens A e 17 linhagens restauradoras, em experimentos conduzidos em Goianira (GO, no delineamento de blocos ao acaso com três repetições. Observou-se a existência de variabilidade genética entre as restauradoras para a produtividade de grãos. Todos os híbridos apresentaram heterose positiva para produtividade de grãos e os de maiores valores tiveram heterose positiva para diferentes componentes de produtividade, indicando que, para esta característica, a heterose é o resultado da interação de heteroses dos componentes de produtividade. Para perfilhos e panículas por metro quadrado, os valores positivos e negativos foram semelhantes em números e magnitude nos três tipos de heterose. O comprimento das panículas e a massa de 100 grãos foram as variáveis que mais contribuíram para a heterose em produtividade de grãos. Todos os híbridos foram mais precoces que os genitores. A heterose para altura de plantas não alterou significativamente o porte dos híbridos porque todos os genitores eram baixos. A hibridação, mesmo entre cultivares do grupo Indica, aumentou a esterilidade de grãos.

    PALAVRAS-CHAVE: Oryza sativa; arroz híbrido; produtividade de grãos; componentes de rendimento.

    The objective of this study was to evaluate heterosis, heterobeltiosis and standard heterosis for eight characteristics of irrigated rice (Oryza sativa L. hybrids derivated from crosses among two A lines and 17 restorers lines. Field trials were conducted in Goinira, Goiás, Brazil. The experimental

  19. Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida

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    Claudine Maria de Bona

    2009-05-01

    . Protoplastos doadores foram expostos a raios gama e protoplastos receptores foram tratados com 3 mmol L-1 de iodoacetamida (IOA e fusionados para hibridação assimétrica. Embriões assimétricos foram germinados e as brotações produzidas foram enxertadas sobre porta-enxertos de sementes de 'Laranja azeda', 'Limão rugoso' ou citrumelo 'Swingle' (C. paradisi x Poncirus trifoliata x 'Sunki' ou enraizadas após embebição em solução de ácido indol-3-butírico (IBA e aclimatadas em casa de vegetação. A análise de ploidia foi realizada por meio de citometria de fluxo e a hibridicidade foi confirmada por análise de polimorfismo de comprimento de fragmentos amplificados (AFLP de amostras de DNA das plântulas. A melhor combinação doador-recipiente foi de protoplastos de 'Ruby Red' irradiados com 80 Gy, fusionados com protoplastos de 'Succari' tratados por 20 min com IOA. Plantas tetraploides e aneuploides foram produzidas e o problema de recalcitrância ao enraizamento foi resolvido pela introdução das bases das brotações em 3.000 mg L-1 de IBA por 10 min.

  20. A língua inglesa no ensino fundamentl I público: diálogos com Bakhtin por uma formação plurilíngue The english language at the public primary education: dialogue with Bakhtin for a pluralistic pedagogy

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    Cláudia Hilsdorf Rocha

    2009-12-01

    Full Text Available Este artigo tem como objetivo principal a discussão sobre as contribuições das teorias do círculo bakhtiniano à área de ensino-aprendizagem de línguas estrangeiras (HALL et al., 2005, no que diz respeito aos primeiros anos da educação formal no Brasil, mais precisamente, ao Ensino Fundamental I. Até o presente momento as línguas estrangeiras, inclusive a língua inglesa, foco deste trabalho, não fazem parte da Matriz Curricular desse âmbito educacional. Frente à importância do inglês em uma sociedade globalizada e à despeito dos controversos impactos de sua influência nos mais variados campos, temos presenciado, de um modo geral, a crescente expansão da implantação dessa língua nas séries iniciais do ensino público, o que vem ocorrendo de forma irregular e sem apoio de parâmetros oficiais (ROCHA, 2006. Diante do exposto, a relevância deste estudo recai na apresentação de algumas diretrizes para o contexto focalizado, que visam a sustentar um ensino-aprendizagem supostamente mais efetivo, situado e significativo. Assim sendo, com base em uma abordagem plurilinguística e pluricultural frente à educação de línguas, tomam-se aqui os gêneros discursivos como organizadores do processo. Entendemos que, em um ensino de caráter dialógico e trans/intercultural (MAHER, 2007, voltado ao desenvolvimento de multiletramentos (COPE e KALANTZIS, 2000 críticos (COMBER, 2006, possa certamente ocorrer a hibridação de gêneros e de culturas. Nesse contexto, torna-se possível a criação de terceiros espaços (KOSTOGRIZ, 2005; KUMARAVADIVELU, 2008 que, por sua vez, propiciam a realização de uma aprendizagem transformadora. Nessa perspectiva, o inglês é visto como um objeto fronteiriço (STAR e GRIESEMER, 1989, no e pelo qual o plurilinguismo, a diversidade e a polifonia naturalmente se fazem presentes.This article aims at discussing the contributions of the Bakhtinian Circle theories to foreign language teaching and

  1. Heterotic parameterizations of crosses between tropical and temperate lines of popcorn = Parametrizações heteróticas de cruzamentos entre linhagens tropicais e temperadas de milho pipoca.

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    Vanessa Quitete Ribeiro da Silva

    2011-04-01

    os hibridos foram avaliados em delineamento em blocos ao acaso, com tres repeticoes. Para as fontes de variacao linhagens eheterose houve significancia, pelo teste F, para todas as caracteristicas. No desdobramento da fonte de variacao heterose, estimativas de heterose media foram significativas para todas ascaracteristicas. Em relacao a heterose de linhagens e especifica, apenas a caracteristica capacidade de expansao nao expressou diferenca significativa, o que confirma que os efeitos heteroticos naosao favoraveis para sintese de hibridos com superioridade. A relacao direta entre .i e i V. ficou claro, especialmente para as caracteristicas pouco influenciadas pelos efeitos de dominancia. O efeito genico aditivo foi confirmado para capacidade de expansao. As combinacoes hibridas revelaram estimativas positivas de heterose para GY, mas nao para PE. Os hibridos P1 x P2 e P3 x P4 tiveram as melhores respostas para GY e PE. A superioridade da combinacao P1 x P3, demonstra que a adicao de genomas com adaptacao edafoclimaticas diferentes e importante para a obtencao de hibridos superiores.

  2. Genotypes selection of Dendrobium (Orchidaceae in vitro propagation phase/ Seleção de genótipos de Dendrobium (Orchidaceae na fase de propagação in vitro

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    Lúcia Sadayo Assari Takahashi

    2004-05-01

    Full Text Available The process of artificial hibridization in orchids is used to obtain new varieties. The objective of the present paper was to select genotypes of Dendrobium favourable for in vitro propagation for commercial utilization through crossings and self polinization of selected matrice plants. Fifteen different plants with contrasting colors, flower sizes and plant heighs were used; the flowers were artificially polinated and the seeds germinated in vitro on MS medium, with half concentration of nutrients. The following crossings were made D9 x D7; D11 x Df22; D6 x D15; D9 x D2; D16 x D15; D5 x D9; D14 x D7; D7 x D14; D4 x D9; D6 x D9; D3 x D8; and the self polinizations: Df22; D7; D5; D21; D2; D15; D8; D23; D3 and D6. The obtained plants were avaluated for: plant heigh, number of roots, fresh and dry matter weight. The best results was observed in the following crosses D9 x D7; D11 x Df22; D6 x D15; D16 x D15 and D14 x D7 and for self polinizations Df22 and D7.O processo de hibridação artificial em orquídeas é utilizado para obtenção de novas variedades. O trabalho teve como objetivo a seleção de genótipos de Dendrobium favoráveis à propagação in vitro em escala comercial através de cruzamentos e autofecundações de plantas matrizes selecionadas. Foram utilizadas 15 plantas com características contrastantes de coloração, tamanho das flores e altura da planta; as flores polinizadas artificialmente e as sementes germinadas in vitro em meio MS, com metade da concentração dos macronutrientes. Foram realizados os cruzamentos: D9 x D7; D11 x Df22; D6 x D15; D9 x D2; D16 x D15; D5 x D9; D14 x D7; D7 x D14; D4 x D9; D6 x D9; D3 x D8; e as autofecundações: Df22; D7; D5; D21; D2; D15; D8; D23; D3 e D6. As plantas resultantes do cultivo foram avaliadas para as características: altura da planta, número de raízes, peso das massas fresca e seca total. Os melhores resultados para as características analisadas foram observados nos

  3. Interfases adhesivas al utilizar cementos de resina en el interior de los conductos radiculares: Comparación al MEB Adhesive interfaces when using resin cement at the interior of the radicular duct: A SEM comparison

    Directory of Open Access Journals (Sweden)

    V. Valenzuela Aránguiz

    2013-02-01

    Full Text Available Se realizó una comparación micro morfológica de la interfase dentina radicular - resina de cementación de postes de fibra de vidrio con dos sistemas de resinas compuestas, Relyx UnicemTM y ParaCemTM. Veinte premolares unirradiculares, extraídos por indicación ortodóncica fueron divididos al azar en dos grupos de 10 y se les realizó terapia endodóntica convencional. Las muestras fueron desobturadas parcialmente y se cementaron los postes de fibra (Tenax Fiber White, Coltene-Whaledent con los materiales en estudio siguiendo las indicaciones del fabricante. Posteriormente se realizaron cortes en los tercios apical, medio y cervical y se prepararon para la observación al microscopio electrónico de barrido (MEB. Las raíces cuyos postes fueron cementados con Relyx UnicemTM no evidenciaron formación de capa hibrida en ninguno de los tercios. La interfase cemento-dentina se encontró irregular y discontinua, en cambio se encontró una intima unión poste-cemento. Las raíces en las que se utilizó el ParaCem formaron una capa híbrida uniforme y homogénea de un espesor promedio de 2 μm sólo en el tercio cervical, no encontrándose esta estructura en los tercios medio y apical. También se evidenció una intima unión poste-cemento. Conclusiones: El cemento Relyx UnicemTM, no ha formado capa híbrida a lo largo del conducto. En cambio con el ParaCemTM es posible encontrarla aunque solo a nivel cervical. No se observó formación de tags con los cementos probados en este estudio. Ambos cementos han generado unión íntima entre el poste de fibra y la resina cementante.A micro morphological study was performed; this study compared the interface between radicular dentin and resin cement, when fixing fiberglass posts with Relyx UnicemTM and ParaCemTM systems. Twenty single-rooted premolars, extracted by orthodontic indication, were divided randomly into two groups of 10 teeth each. Endodontic treatment using gutta-percha points and Grossman

  4. Ecología y distribución de Senecio pterophorus (Compositae en la Península Ibérica

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    Chamorro, Lourdes

    2006-06-01

    ógica en relación con el hábitat, de modo que en ambientes riparios y ruderales los individuos desarrollan un porte esférico, de menor tamaño, y unas hojas más pequeñas que en ambientes forestales, donde el porte de las plantas es más alargado y el tamaño de las hojas mucho mayor. Esta especie, en creciente expansión, debe ser considerada una planta invasora en la Península Ibérica, ya que ha aumentado considerablemente el número de sus poblaciones en un período relativamente corto. Además, se sugiere que S. pterophorus puede llegar a ser una amenaza para la conservación de la diversidad de especies y de hábitats naturales autóctonos, como ocurre en Australia, donde desplaza a las especies nativas e incluso se hibrida con otras especies de Senecio.

  5. Desempenho de cultivares de cenoura em função da água no solo Performance of carrot cultivars depending on soil water

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    Joaquim A. Lima Junior

    2012-05-01

    Full Text Available RESUMO Objetivou-se, com este estudo, avaliar o efeito de diferentes tensões da água no solo sobre a produção de duas cultivares de cenoura irrigadas por gotejamento. O experimento foi conduzido em Lavras, Minas Gerais (21º 14' S, 45º 00' W e 918,8 m, no período de julho a outubro de 2010. O delineamento experimental utilizado foi em blocos casualizados, em esquema fatorial 2 x 6, com quatro repetições. Os tratamentos se constituíram de duas cultivares de cenoura, cultivar não híbrida Nantes e cultivar hibrida Nayarit F1, além de seis níveis críticos de tensão da água no solo, 15, 25, 35, 45, 60 e 75 kPa. Com os resultados concluiu-se que o híbrido Nayarit F1 apresentou melhores respostas com relação às seguintes características analisadas: produtividade total de raízes, produtividade de raízes comerciais, massa média de raízes comerciais e eficiência no uso da água. Visando à obtenção de maiores produtividades de raízes (total e comercial e maior massa média de raízes comerciais, em ambas as cultivares, deve-se irrigar no momento em que a tensão da água no solo estiver em torno de 15 kPa na profundidade de 0,15 m. O valor mínimo encontrado para a raízes do tipo refugo ocorreu na tensão de 21 kPa, resultando em um valor mínimo de massa raiz de 41,67g.ABSTRACT Different soil water tension values were tested to evaluate the effects on yield of two drip irrigated carrot cultivars. The experiment was carried out at Lavras, in the State of Minas Gerais, Brazil (21º 14' S, 45º 00' W and 918.8 m, from July to October 2008. The statistical design used was randomized blocks in a 2 x 6 factorial scheme with four replications. The treatments consisted of two cultivars of carrot (hibrid in Nayarit F1 and non hybrid Nantes and six critical soil water tension levels (15, 25, 35, 45, 60 and 75 kPa. It can be concluded that the hybrid Nayarit F1 presented the best response in relation to the following analyzed variables

  6. Determinação do modo de reprodução em capim-gordura (Melinis minutiflora Beauv. por padrões enzimáticos Detection of the mode of reproduction in molasses grass (Melinis minutiflora Beauv. by enzyme patterns

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    Lia Rejane Machado Silveira

    1996-04-01

    Full Text Available Com o objetivo de determinar o modo de reprodução do capim-gordura (Melinis minutiflora Beauv., 24 ecótipos da coleção da Universidade Federal de Viçosa foram utilizados em cruzamentos, após emasculação massal por imersão da inflorescência em água quente, testando-se quatro faixas de temperatura. Analisaram-se, comparativamente, os padrões de isozimas dos progenitores e da descendência proveniente de panículas de polinização aberta e de panículas submetidas aos tratamentos de proteção; polinização e proteção; emasculação e proteção; e emasculação, polinização e proteção. A hipótese da apomixia ser o mecanismo reprodutivo operante no capim-gordura foi bastante favorecida pela extensiva uniformidade da progênie e semelhança isozimática com o progenitor feminino. Contribuiu para isso o fato de toda a população amostrada apresentar um padrão de bandas característico de heterozigotos para os sistemas enzimáticos leucina aminopeptidase e malato desidrogenase, uma vez que a apomixia, ao contrário da autofecundação, facilita a manutenção da heterozigosidade. Segregação isozimática entre plantas dos ecótipos CG4 e CG5, relativamente ao sistema fosfatase ácida, sugere, no entanto, a ocorrência de alguma porcentagem de fecundação cruzada, que foi confirmada pela detecção de hibridação entre os ecótipos CG2 e CG13 nas isoesterases. Esses resultados conduzem à conclusão de que um mecanismo facultativo (parcialmente sexual de apomixia atua na reprodução do capim-gordura.Panicles of 24 molasses grass ecotypes were used in an experiment involving: bagging before anthesis; cross-pollination and bagging; emasculation and bagging; and emasculation, cross-pollination and bagging. Emasculation was accomplished by immersing the inflorescences into warm water, four temperature levels, for 12 minutes. The breeding system was accessed by comparing the isozyme patterns of the estereases, acid phosphatases

  7. Repetibilidade da produção de cachos de híbridos interespecíficos entre o caiaué e o dendezeiro Repeatability for bunch production in interspecific hybrids between caiaué and african oil palm

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    Gilson Sánchez Chia

    2009-01-01

    Full Text Available A hibridação interespecífica entre o caiaué (Elaeis oleífera (Kunth Cortés e o dendezeiro (E. guineensis Jacq. tem sido explorada com o objetivo de desenvolver cultivares tão produtivas quanto as de dendezeiro, aliada à resistência a pragas e doenças, principalmente o amarelecimento fatal, elevada taxa de ácidos graxos insaturados e redução de porte características do caiaué. Por ser uma cultura perene com longo ciclo de produção, além dos altos custos para manutenção e avaliação dos experimentos de melhoramento genético, é necessário definir o período mínimo de avaliação para que a seleção dos híbridos seja realizada com eficiência e mínimo dispêndio de tempo e recursos. Este estudo teve como objetivo estimar os coeficientes de repetibilidade dos caracteres número de cachos, peso total de cachos e peso médio de cachos de híbridos interespecíficos e definir o número de anos consecutivos de avaliação necessário para seleção eficiente dos melhores cruzamentos e indivíduos. Os coeficientes de repetibilidade foram estimados pelos métodos da análise de variância, componentes principais com base na matriz de covariância (CPCV e de correlações, e análise estrutural com base na matriz de correlações. O método dos CPCV demonstrou ser o mais adequado para o estudo da repetibilidade da produção de cachos, indicando quatro anos consecutivos de avaliação para selecionar progênies, representadas por dez plantas, com coeficientes de determinação (R² superiores a 85%, e que para seleção individual de plantas são necessários pelo menos seis anos consecutivos de avaliação para atingir R² superior a 80%.Interspecific hybridization between the caiaué (Elaeis oleífera (Kunth Cortés and the african oil palm (E. guineensis Jacq. has been exploited with the objective of developing varieties as productive as African oil palm and with the pest and disease resistance, reduced height and high levels

  8. Technical performance of the Villas Carrousel PV-Wind hybrid systems

    Energy Technology Data Exchange (ETDEWEB)

    Agredano, J.; Munguia, G.; Flores, J. R. [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1997-12-31

    la energia producida se usa mediante lamparas fluorescentes de alta eficiencia de 12V, 13W. Los sistemas se disenaron para producir de 140 a 180 Ampere-horas por dia. Durante el primer mes de operacion se presentaron algunos problemas con la medicion del voltaje de las baterias. Este parametro era anteriormente medido en la barra colectora de CD del tablero de control. Alli se detectaron algunos problemas de corrosion. Este problema dio como resultado que los bancos de baterias no se cargaran completamente, y en varios casos se observo operacion anormal de los aerogeneradores. En general, los sistemas producen la energia demandada por la carga. Esta primera experiencia esta ayudando a promover la tecnologia Mini Hibrida para otras aplicaciones. Este articulo presenta alguno de los resultados del monitoreo del sistema durante el primer ano de operacion, lo que da una idea general del rendimiento del sistema.

  9. Protoplast production and isolation from Etlingera elatior=Produção e isolamento de protoplasto de Etlingera elatior

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    Milene Alves de Figueiredo Carvalho

    2012-01-01

    Full Text Available The technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M. The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh matter. The optimal incubation period was 15 hours. The in vitro leaves presented a greater viability (96% and larger protoplasts (36.7 µm diameter. Greater yields were obtained using a rotatory system with protoplasts incubated in the dark. The best enzymatic combination was 3% Cellulase “Onozuca” R-10 + 2% Meicelase + 1% Driselase + 1% Dextran + 5 mM MES, followed by the addition of 0.6 M mannitol.Com o objetivo de realizar hibridações que auxiliam em programas de melhoramento genético de flores ornamentais, protoplastos foram isolados a partir de diferentes tecidos (folhas in vitro, pseudocaules in vitro e folhas em sistema hidropônico de Etlnigera elatior (Jack R. M. Smith. Foram testados seis diferentes combinações enzimáticas, quatro períodos de incubação, sistema rotatório (40 rpm ou estacionário no escuro, concentrações de manitol (0,5; 0,6 e 0,7 M, o diâmetro e a viabilidade dos protoplastos isolados. A melhor fonte de explante utilizado no isolamento de protoplastos foi folha in vitro, com rendimento de 22 x105 protoplastos g-1 MF. O melhor tempo de incubação foi 15 horas, pois períodos superiores a este causavam diminuição no rendimento e viabilidade dos

  10. Psicanálise e neurociências: um mapa dos debates Psicoanálisis y neurociencias: un mapa de los debates Psychoanalysis and neurosciences: a map of the debates

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    Marcia Moraes Davidovich

    2010-12-01

    Full Text Available Este artigo pretende apresentar um mapa panorâmico dos debates em torno da relação e da possível articulação entre a psicanálise e as neurociências. No campo psicanalítico são identificados três grupos principais, definidos a partir de seus pressupostos: (1 hibridação, (2 isolamento e (3 interlocução. O primeiro grupo entende ser necessária a construção de um campo híbrido, já que as neurociências poderiam fornecer à psicanálise fundamentos e instrumentos metodológicos e conceituais mais sólidos sobre o funcionamento psíquico. Esta proposta vem sendo desenvolvida com a fundação da neuropsicanálise. Já o segundo grupo, marcado fortemente pelo discurso lacaniano, é refratário a qualquer forma de articulação, por considerá-las todas epistemologicamente inviáveis, resultando necessariamente em uma submissão da psicanálise aos ditames cientificistas atuais. Por fim, o terceiro grupo entende ser a interlocução, sem a hierarquização dos modelos em jogo, fonte fértil para a formulação de novas hipóteses teóricas e para a revisão do edifício conceitual da psicanálise.Este artículo pretende presentar un mapa panorámico de los debates sobre la relación y la posible articulación entre Psicoanálisis e Neurociencias. En el campo psicoanalítico, son identificados tres grupos principales, definidos a partir de sus presuposiciones: (1 hibridismo, (2 aislamiento e (3 interlocución. El primer grupo entiende como necesaria la construcción de un campo híbrido, ya que las Neurociencias podrían suministrar a la Psicoanálisis fundamentos e instrumentos metodológicos y conceptuales más sólidos sobre el funcionamiento psíquico. A su vez el segundo grupo, marcado fuertemente por el discurso lacaniano, es refractario a cualquier forma de articulación por considerarla epistemológicamente inviable y resultando necesariamente en una sumisión de la psicoanálisis al dictamen cientificista actual. Por último

  11. Efeito da autofecundação em cultivares de abacaxi Effects of self pollination in pineapple cultivars

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    José Renato Santos Cabral

    2003-04-01

    Full Text Available O elevado nível de heterozigose dos parentais utilizados em hibridações é a principal causa da baixa eficiência dos programas de melhoramento genético do abacaxizeiro em gerar novas cultivares. Por outro lado, os efeitos da autofecundação são pouco conhecidos em abacaxi, mas esta estratégia pode proporcionar avanços significativos no melhoramento desta planta. Assim, este trabalho teve como objetivo observar os efeitos da autofecundação em cultivares de abacaxi. Inflorescências das cultivares Primavera, Perolera, Roxo-de-Tefé, Pérola e Smooth Cayenne foram protegidas antes da antese para possibilitar a ocorrência de autofecundação. As sementes produzidas foram germinadas em câmara de crescimento, utilizando-se como substrato do meio de cultura MS, suplementado com 30 g.L-1 de sacarose e solidificado com 2 g.L-1 de "Phytagel. Durante a germinação, observou-se que cerca de 16% das sementes de 'Roxo-de-Tefé' produziram plântulas albinas. Foram obtidas 43 plantas a partir de 'Primavera', cinco de 'Perolera', onze de 'Roxo-de-Tefé' e nenhuma de 'Pérola' e 'Smooth Cayenne'. Todas as plantas da progênie de 'Primavera' apresentaram folhas sem espinhos nos bordos ("piping", evidenciando homozigose para esta característica, enquanto na progênie de 'Perolera' três plantas evidenciaram folhas sem espinhos nos bordos e duas com folhas espinhosas, segregando para o caráter espinescência. Na descendência de 'Roxo-de-Tefé', oito plantas apresentaram folhas de cor roxa e três com folhas verdes, com segregação para a cor da folha, mas todas evidenciaram folhas com espinhos no bordo. As baixas porcentagens de germinação, o crescimento lento e o baixo vigor observados nas plantas em casa de vegetação, viveiro e campo, evidenciaram a ocorrência de depressão por endogamia nestas fases de desenvolvimento.The high level of heterozygosis of parentals used to generate pineapple hybrids has resulted in low efficiency of the

  12. Caracterização molecular e variabilidade genética de acessos elite de mandioca para fins industriais Molecular characterization and genetic variability of elite cassava accessions for industrial purpose

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    Eduardo Alano Vieira

    2010-12-01

    Full Text Available Marcadores moleculares são ferramentas úteis na caracterização molecular de acessos de mandioca, em razão de apresentarem elevada capacidade de detecção das informações contidas no genoma. O objetivo deste trabalho foi caracterizar, por meio de marcadores RAPD, 20 acessos de mandioca para fins industriais conservados no Banco Regional de Germoplasma de Mandioca do Cerrado (BGMC. Em laboratório, os acessos foram avaliados por meio de marcadores RAPD, sendo posteriormente estimada a matriz de similaridade genética entre os acessos, por meio do índice de Jaccard. A análise, feita através de 11 iniciadores, gerou um total de 120 marcadores RAPD, dos quais 74 (62% foram polimórficos, revelando a presença de elevada variabilidade genética no grupo de acessos avaliados. A análise de agrupamento revelou a formação de apenas um agrupamento forte, formado pelos acessos BGMC 1130, BGMC 788, BGMC 1270 e BGMC 1107, o que indica que, no melhoramento genético de mandioca, não devem ser priorizadas hibridações entre esses acessos, sob pena de efeitos de endogamia. Por sua vez, o acesso BGMC 436 foi o mais divergente em relação aos demais e, como expressa elevado potencial produtivo na região do Cerrado do Brasil Central, representa boa opção como genitor para o melhoramento de mandioca para essa região. O estudo comprovou que os marcadores RAPD são eficientes na determinação da variabilidade genética dos acessos avaliados e que neste grupo existe elevada variabilidade genética passível de ser utilizada no melhoramento genético.Molecular markers are useful tools for the molecular characterization of cassava accessions since they present high capacity to detect information within the genome. The aim of this research was to characterize through RAPD molecular markers, 20 industrial cassava accessions conserved in the Cerrado Cassava Regional Germoplasm Bank ("Banco Regional de Germoplasma de Mandioca do Cerrado"-BGMC. Upon

  13. A new model for commercially sustainable renewable energy-based rural electrification in Indonesia

    Energy Technology Data Exchange (ETDEWEB)

    Walt, Robb [Integrated Power Corporation-Indonesia, (United states)

    1995-12-31

    ] Las rapidas crecientes demandas y los requerimientos de acceso a la electricidad por todas las areas remotas de Indonesia juntamente con los subsidios de mas de $500 millones de dolares para la electrificacion rural han forzado al gobierno de Indonesia a buscar alternativas al modelo convencional de empresa de servicios electricos para la electrificacion rural. En 1992-1993 se llevo a cabo un estudio en colaboracion con la Agencia para la Aplicacion y la Evaluacion de Tecnologia (BPPT)del Gobierno de Indonesia y la Empresa Nacional de Energia Electrica (PLN) para apoyar la busqueda de soluciones sustentables para la electrificacion de comunidades remotas. Este estudio produjo un Nuevo Modelo comercial para la electrificacion de comunidades rurales fuera de la red en Indonesia con servicios de la calidad de empresa electrica. Este nuevo modelo esta caracterizado por el uso de nuevas tecnologias para la generacion, distribucion y ventas de electricidad. La clave del exito del nuevo modelo son las plantas electricas hibridas basadas en energia renovable y en el uso de medidores flexibles suministradores de energia bajo demanda. Las tarifas estimadas para el servicio de electricidad estan basadas en las cantidades que actualmente se pagan por los propietarios de casas rurales por keroseno, velas y servicios de baterias a diferentes niveles de ingreso. El estudio mostro que la mayor parte de los propietarios de casa estan deseosos y tienen la capacidad para pagar cantidades adicionales por valiosos servicios confiables de electricidad del grado de empresa de servicios electricos, tales como mejor alumbrado, entretenimiento de television y para usos productivos (economicos) durante las horas diurnas. Se llevo a cabo una evaluacion financiera en sistemas hibridos de energia electrica en comunidades fuera de la red con ganancias generadas en base a las tarifas del mercado y cobradas mediante nueva tecnologia para la compra de electricidad y pago anticipado en base a un

  14. Análise da recuperação do genitor recorrente em maracujazeiro-azedo por meio de marcadores RAPD Recovery analysis of recurrent genitor in sour passion fruit through RAPD markers

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    Kenia Gracielle da Fonseca

    2009-03-01

    Full Text Available O Brasil é o maior produtor mundial de maracujá, entretanto tem-se observado redução na produtividade do maracujazeiro nos últimos anos, devido, principalmente, a fatores fitossanitários. Na Embrapa Cerrados, a transferência de genes de resistência de espécies silvestres para as comerciais de maracujazeiro tem sido feita por meio de hibridações interespecíficas seguidas de um programa de retrocruzamentos auxiliados por marcadores moleculares. Este trabalho teve por objetivo verificar a recuperação do genoma recorrente nas plantas RC4 e RC5 [(Passiflora edulis x Passiflora setacea x Passiflora edulis ] com base em marcadores RAPD. O estudo foi desenvolvido no Laboratório de Genética e Biologia Molecular da Embrapa Cerrados. Amostras de DNA de cada material genético (17 plantas RC4, 16 plantas RC5, Passiflora edulis e Passiflora setacea foram amplificadas para obtenção de marcadores RAPD. Foram utilizados 12 primers decâmeros para as plantas RC4 e 14 primers decâmeros para as plantas RC5. Os marcadores RAPD gerados foram convertidos em matriz de dados binários. Verificou-se alta porcentagem de marcadores polimórficos em consequência do cruzamento-base interespecífico. A menor similaridade genética foi observada entre as espécies P. edulis e P. setacea, evidenciando a grande distância genética dessas espécies.Brazil is the largest world producer of passion fruit, however, it has been observed a reduction in the productivity in recent years due, mainly, to phytosanitary factors. At Embrapa Cerrados, the transfer of resistance genes from wild to commercial species of passion fruit has been made through interspecific hybridations, followed by a backcrossing molecular marker-assisted program. The objective this work was to verify the recovery of recurrent genome at the plants RC4 and RC5 [(Passiflora edulis x Passiflora setacea x Passiflora edulis] based on RAPD markers. The study was developed at Embrapa Cerrados

  15. Melhoramento do cafeeiro: XXXI - Ensaio de populações F2 de híbridos entre cultivares de Coffea arabica Coffee breeding: XXXI - Results of a field trial comprising several F2 populations derived from hybridization between cultivars of Coffea arabica

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1967-01-01

    Full Text Available São apresentados dados referentes à produção de café cereja em seis anos consecutivos, de um ensaio em que seis populações F2 derivadas dos cultivares 'Mundo Novo', 'Bourbon Amarelo', 'Bourbon Vermelho', 'Caturra Amarelo' e 'Caturra Vermelho' são analisadas. Duas populações destacaram-se pela produtividade e tipo de planta, e alguns de seus representantes foram escolhidos para estudo da F3 e prosseguimento do plano de melhoramento. Dessas populações a mais promissora corresponde à F2 da hibridação entre a planta CP 374-19 de 'Mundo Novo' e CJ 24-6 de 'Bourbon Amarelo'. Analisaram-se as variações da produção em cada um dos seis anos consecutivos, separadamente e em conjunto, e também os dados referentes à produção acumulada de dois e quatro primeiros anos, a fim de compará-los com os dados de produção total dos seis anos de produções consecutivas. Os resultados mostram que a classificação das populações depois do quarto ano é bastante semelhante à obtida após a análise de seis anos de produção. A análise das plantas individuais mostrou que nas populações F2 estudadas é bastante reduzida a proporção de cafeeiros valiosos para prosseguimento da seleção.The existence of several major genetic factors affecting important agronomic characteristics has led the coffee breeders to use them in their breeding program. The gene Ct (reduced growth and xc (yellow pericarp and better out-turn have been used rather extensively in an attempt to reduce the height of the 'Bourbon Amarelo' and improve the out-turn of the 'Mundo Novo' cultivars. Some data of a series of hybrids between 'Bourbon Amarelo', 'Caturra Amarelo', 'Caturra Vermelho', 'Bourbon Vermelho' and 'Mundo Novo' are discussed in the present paper. As expected, due to the origin of these cultivars, the F2 generation showed little genetic variation. Some of the new recombinants carrying the desirable major genetic factors, were shown to be inferior to the

  16. Energy analysis of a solar advanced refrigeration system; Analisis energetico de un sistema de refrigeracion solar avanzado

    Energy Technology Data Exchange (ETDEWEB)

    Velazquez Limon, Nicolas [Universidad Autonoma de Baja California (Mexico); Best y Brown, Roberto [Universidad Nacional Autonoma de Mexico, Temixco, Morelos (Mexico)

    2000-07-01

    In this article is presented a solar refrigeration system that integrates the more recent and important technological advances of the training solar systems (SCS) and of the advanced absorption units. An analysis and evaluation is made of the energy behavior of the absorption system with heat exchanger absorber-generator (GAX), air cooled and assisted by a hybrid power plant natural gas-solar. Given the characteristic of high not-linearity of the resulting system of occupations, the proposed methodology contemplates a calculation sequence for the external currents and an iterative procedure for the internal currents. The unit was designed with a capacity of 10.6 kw (3 tons.) of cooling and uses ammonia-water as working fluid. Giving priority to internal energy integration, an arrangement of the GAX cycle is proposed, that allows 19% of solar contribution at full load, being able to be greater at partial loads. In spite of using as cooling means air at 40 Celsius degrees with a relative humidity of 24%, a COP of 0.86 in the cooling mode was obtained and 1.86 in the heating mode, with an internal energy integration of 1013 kJ/min, 37% more of the energy that is supplied in the generator. The massic flow rates of the GAX cycle were compared with those of a basic cycle, resulting 73% and 62% lower for the circulation rate and for the flow rate, respectively. [Spanish] En este articulo se presenta un sistema de refrigeracion solar que integra los avances tecnologicos mas recientes e importantes de los sistemas de capacitacion solar (SCS) y de las unidades de absorcion avanzadas. Se realiza un analisis y evaluacion del comportamiento energetico del sistema de absorcion con intercambio de calor absorbedor-generador (GAX), enfriado por aire y asistido por una fuente de energia hibrida gas natural-solar. Dada la caracteristica de alta no-linealidad del sistema de ocupaciones resultante, la metodologia propuesta contempla una secuencia de calculo para las corrientes externas

  17. Utilização de marcadores moleculares RAPD e EST's SSR para estudo da variabilidade genética em cana-de-açúcar¹ Use of molecular markers RAPD, and ESTs SSR to study genetic variability in sugarcane

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    João Andrade Dutra Filho

    2013-03-01

    Full Text Available Marcadores moleculares dos tipos RAPD e EST's SSR foram utilizados como ferramentas para avaliar a variabilidade bem como estimar a divergência genética entre variedades comerciais e clones de cana-de-açúcar oriundos via autofecundação. Vinte e três genótipos foram utilizados neste estudo oriundos do Programa de Melhoramento Genético da Cana-de-açúcar da Rede Interuniversitária para o Desenvolvimento do Setor Sucroalcooleiro (PMGCA/RIDESA. A extração do DNA genômico seguiu a metodologia CTAB com modificações para cana-de-açúcar. Foram utilizados 11 oligonucleotídeos RAPD da operon Technologies e 7 EST's SRR obtidos através de uma extensa revisão de literatura. As análises da divergência genética foram realizadas com o auxílio do Programa GENES. Os marcadores RAPD detectaram um alto grau de polimorfismo genético, produzindo 61 bandas, das quais 58 foram polimórficas. Os marcadores EST's SSR amplificaram 38 alelos, sendo 34 polimórficos. Havendo a formação de três grupos, com a população estudada. A maior parte da variação genética foi mantida dentro das progênies, evidenciando a ocorrência de um alto grau de variabilidade genética entre os genótipos de cada progênie para fins de melhoramento. Através da divergência genética estimada foi possível identificar parentais divergentes para trabalhos de hibridação, visando a obtenção de clones superiores com caracteres de interesse à agroindústria canavieira. Os marcadores molecuares RAPD e EST's SSR foram igualmente eficientes para estimar a variabilidade genética nos genótipos testados e elaborar os cruzamentos a serem realizados nos programas de melhoramento.Molecular markers of the type RAPD and ESTs SSR were used as tools to evaluate the variability, and estimate the genetic divergence between commercial varieties and sugarcane clones from self-pollination. Twenty-three genotypes from the Program for the Genetic Improvement of Sugarcane of the

  18. Melhoramento da cana-de-açúcar: IX: evaluation of clones obtained in 1980 and 1981 hybridizations, selected in Ribeirão Preto region, state of São Paulo, Brazil Sugarcane breeding: IX

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    Marcos Guimarães de Andrade Landell

    1995-01-01

    Full Text Available Testaram-se doze clones de cana-de-açúcar, obtidos de hibridações realizadas em Camamu (BA, em 1980 e 1981, em três ensaios na região de Ribeirão Preto (SP. Além do delineamento em blocos ao acaso, com seis repetições, efetuou-se a análise estatística com a média das três colheitas (1.°, 2.° e 3.° cortes, tomando as variedades SP70-1143, NA56-79 e IAC64-257 como testemunhas-padrão. Entre os caracteres agroindustriais avaliados estão: a produtividade de cana e açúcar, pol % cana, fibra %, intensidade de florescimento, índice de infestação de broca-do-colmo (Diatraea saccharalis e a reação à ferrugem (Puccinia melanocephala. O melhor clone foi o IAC80-2094, indicado para início de safra, com boa produção e bom teor de fibra, mas de florescimento intenso e suscetibilidade à ferrugem. O IAC81-2004 também apresentou bons resultados, caracterizando-se como precoce, com bom teor de fibra e boa resistência à broca-do-colmo. Em condições naturais de campo, porém, sua desvantagem é a grande incidência de "chicotes" de carvão. Apesar de ambos os clones apresentarem características agroindustriais vantajosas, desaconselha-se que sejam incluídos no estudo de manejo parietal para outras regiões paulistas, em função dos problemas fitossanitários citados.A number of sugarcane clones obtained in crosses made in 1980 and 1981, was tested in three locations with Oxisol soils at Ribeirão Preto region. The commercial varieties SP70-1143, NA56-79 and IAC64-257 were used as controls in trials and evaluated for agricultural and industrial traits on the average of three harvests. The best clone in the experiments was IAC80-2094, which has been indicated for early harvest period with good yield and fiber content, but with heavy tasseling and susceptibility to rust. Other early maturing clone was IAC81-2004, which showes good fiber content and stem borer tolerance, however it does show, in natural conditions in the field

  19. Eficiência reprodutiva no amendoim cultivado (Arachis hypogaea L. Reproductive efficiency in the peanut (Arachis hypogaea L.

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    Cândida H. T. Mendes Conagin

    1960-01-01

    Full Text Available Cinco variedades de amendoim, abrangendo os três tipos vegetativos conhecidos, foram comparadas quanto à sua eficiência reprodutiva. Para conhecer o resultado final de porcentagem de frutos e sementes, foram estudadas a quantidade de flôres, a duração e as oscilações do florescimento; a formação de frutos e sementes também foi estudada com detalhes. Tendo variado o número de plantas observadas nos diversos itens, em diversos anos, tôdas as informações foram referidas a "uma planta" para poderem ser comparadas quando necessário. O período útil de florescimento dura as dez semanas medianas do ciclo vegetativo da planta, o máximo de flôres sendo atingido na 10.ª semana dêsse ciclo. Há, entretanto, a observar, que as variedades eretas são mais precoces do que as de porte decumbente; o florescimento apresenta bruscas oscilações diárias. É a variedade Tatuí a que produz mais flôres por planta, tendo, entretanto, a mais baixa eficiência reprodutiva. As outras variedades têm eficiência mais alta, mas, mesmo assim, ela é surpreendentemente baixa, em se tratando de uma planta de grande florescimento como é o amendoim. As informações obtidas nestes ensaios são fundamentais para qualquer trabalho de melhoramento por hibridação e já estão sendo utilizadas nos atuais cruzamentos, feitos pela Seção de Citologia.A study of the reproductive efficiency of five agronomical varieties of peanut was made. Determinations were made of fruit and seed percentages in relation to number of flowers procured, length of flowering period, alternation of high and low daily flower frequencies, and number of flowers which had no chance to become fruit?. Data obtained supported lhe following conclusions: (a Varieties of the erect vegetative type were early; they began and ended their vegetative am! flowering cycles before those of the decumbent type. (b The flowering curves showed an abrupt alternation of high and low frequencies. (c The

  20. Interculturalidade e literatura de almanaques: o caso do almanaque Abril (1975-2006 - Interculturality and literature of almanacs: the case of almanaque Abril (1975-2006

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    Mateus H. F. Pereira

    2011-03-01

    Full Text Available Resumo No presente texto, procuramos refletir sobre a interculturalidade lançando o olhar para uma publicação que pode ser considerada um dos maiores sucessos editoriais do mercado brasileiro contemporâneo: o Almanaque Abril (1975-2006. Conforme mostraremos, a literatura de almanaque nos parece ser uma fonte privilegiada para a apreensão de certas “culturas híbridas”, uma vez que sua própria definição é caracterizada pelo hibridismo. Palavras-chave: literatura de almanaques; hibridação; Almanaque Abril.   INTERCULTURALITY AND LITERATURE OF ALMANACS: THE CASE OF ALMANAQUE ABRIL (1975-2006 Abstract In this present text we want to reflect about the interculturality, analyzing one publication, the Almanaque Abril (1975-2006, that could be considered one of the most editorials success of Brazilian contemporary market, because it sold approximately 100 thousands almanacs per year, between 1975 and 2006. As we will say, the almanac literature seems to be a privileged source to get some "hybrid cultures", because this type of literature is for definition hybrid. Keywords: Almanac literature; hybridization; Almanaque Abril.   INTERCULTURALIDAD Y LITERATURA DE ALMANAQUES: EL CASO DEL ALMANAQUE ABRIL (1975-2006 Resumen En el presente texto, procuramos reflexionando sobre la interculturalidad lanzando una mirada para una publicación que puede ser considerada uno de los mayores sucesos editoriales del mercado brasilero contemporáneo: el Almanaque Abril (1975-2006. Conforme mostraremos, la literatura de almanaque nos parece ser una fuente privilegiada para la aprensión de ciertas “culturas híbridas”, una vez que su propia definición es caracterizada pelo hibridismo. Palabras clave: literatura de almanaques; hibridación; Almanaque Abril.   INTERCULTURALITÉ ET LITTÉRATURE D’ALMANACH: LE CAS DE L’ALMANACH AVRIL (1975-2006 Résumé Dans cet texte, on a cherché à réfléchir à l’interculturalité tout en observant une

  1. Origin and evolution of cultivated cucurbits Origem e evolução de cucurbitáceas cultivadas

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    Dilson Antônio Bisognin

    2002-08-01

    ção e a exploração da variabilidade genética para o desenvolvimento de germoplasma e de novas cultivares. A seleção durante o processo de domesticação foi para formato de fruto, redução de gosto amargo na polpa, aumento de tamanho e redução do número de sementes e aumento do tamanho de frutos. Esta seleção permitiu a manutenção de grande variabilidade genética entre e dentro das espécies cultivadas, a qual está associada a uma diversidade de usos que requer diferentes formatos, tamanhos e uma constante relação entre comprimento e diâmetro de fruto. A discussão da história do melhoramento genético de cucurbitáceas mostra como a seleção artificial pode aumentar o ganho de seleção para caraterísticas de fruto, para atender usos específicos, e para adaptabilidade às mais diversas regiões do mundo. Apesar de a hibridação interespecífica ter sido amplamente utilizada no melhoramento genético de cucurbitáceas, ainda existe um grande potencial para aumentar seu uso afim de desenvolver germoplasma e novas cultivares.

  2. Prevalência de papilomavirus humano (HPV anal, genital e oral, em ambulatório geral de coloproctologia Prevalence of anal, genital and oral human papillomavirus (HPV in general ambulatory for diseases of colon and rectum

    Directory of Open Access Journals (Sweden)

    João Carlos Magi

    2006-09-01

    Full Text Available O Papilomavirus Humano (HPV anal tem alta prevalência e incidência na população. O objetivo deste trabalho é verificar a prevalência de HPV anal, bucal e genital em ambulatório público de Coloproctologia, com base no exame de captura hibrida. Foram estudados 64 pacientes atendidos no ambulatório geral de Coloproctologia do Hospital Heliópolis, no período de 22/11/2005 a 20/12/2005, onde foram levantados, de forma prospectiva, dados clínicos, pessoais e comportamentais, identificando o perfil do grupo como não sendo de risco para HPV. Nenhum paciente deste grupo veio à consulta com queixa ou apresentando sinais de HPV ou outra doença sexualmente transmissível. Foram colhidos para cada paciente um esfregaço com escova de boca, outro de vulva/pênis e outro do ânus. O resultado mostrou que 15,62% dos pacientes apresentaram HPV anal, bucal ou genital, sendo que 4,68% do total de pacientes apresentavam HPV anal, um paciente de alto risco associado ao HPV genital, outro misto e outro de baixo risco. Trinta por cento dos pacientes HPV+ apresentavam dor anal como queixa principal. A conclusão é que a prevalência de HPV anal, genital e bucal é elevada, sendo que 4,68% apresentavam HPV anal e daqueles HPV positivos 30% apresentavam dor anal na queixa principal.Anal Human Papilomavirus (HPV has a high prevalence and incidence in the population. The purpose of this project is to verify the prevalence of anal, oral and genital HPV at the Coloproctology Clinic, based on the hybrid capture exam. Sixty four patients were assisted at the clinic from November 22nd to December 20th 2005. Clinical, personal and behavioral data of the patients were set up in a prospective way. The group profile was identified as not being a risky one to HPV. No patient of this group, when was assisted, had complained of or had presented any symptoms of HPV or any other sexually transmitted disease (STD. From each patient it was collected a mouth sample, a vulva

  3. Induction of male sterility through manipulation of genetic mechanisms present in vector species of Triatominae. II. Partial restoration of male fertility

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    Alina Perlowagora-Szumlewicz

    1976-12-01

    their spermatecae. The failure of the sperm to migrate to the spermatecae indicate prezygotic pos-copulation incompatibility, thus the hybrid male can't be used to suppress populations. The female hybrids mated with parent males of either species had reduced fertility and ther sons were sterile as were those of their fertile daughters. However, continous backcrossing of the hybrid females and their female progeny to parental males partially restored fertility of the males and increased fertility of females, as scored by egg hatchability. Fertility of hybrid females, measured by the yield of adults capable to reproduce, indicated that the reproductive perfomance decreased when hybrid females and their daughters were backcrossed additional generations to parental males. It is tentatively suggested that hybrid females could be used for suppression if they compete efficiently with wild females.O desenvolvimento de métodos integrados que indicam a libertação de machos estéreis para suplementar as técnicas convencionais com inseticidas, utilizadas no controle de insetos de importância médica, coloca a questão de os vetores da Doença de Chagas possuírem mecanismos naturais pela manipulação dos quais possam os mesmos serem controlados. Resultados de experiências anteriores, previamente publicados, restringiram-se a informações fragmentares, levando várias questões, cujas respostas tornaram-se viáveis neste estudo. Híbridos interespecfficos originaram-se de cruzamentos recíprocos entre T. pseudomaculata e T. sórdida e de cruzamentos unilaterais entre fêmeas T. pseudomaculata e machos T. infestans. Essas fêmeas cruzaram facilmente, revelando, porém, fecundidade diminuida, ainda que a progênie fosse relativamente abundante. Quando fêmeas T. pseudomaculata foram acasaladas com machos T. brasiliensis, a hibridação foi mais difícil, porque poucas das fêmeas cruzaram e aquelas que o fizeram tiveram sua fertilidade altamente reduzida. De todos os

  4. Uma nova forma de Coffea

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    C. A. Krug

    1950-01-01

    Full Text Available Nos extensos trabalhos de melhoramento do cafeeiro, há 18 anos em realização na Subdivisão de Genética do Instituto Agronômico, tem-se dedicado especial atenção à espécie C. arabica L., pelo fato de todos os nossos cafèzais pertencerem a esta espécie que, sem dúvida, fornece o produto de melhor qualidade. Nas regiões de terras extremamente cansadas, um dos principais fatôres levados em consideração no melhoramento é a rusticidade, caráter êsse, entretanto, encontrado de preferência em outras espécies, tais como o C. canephora e C. Dewevrei, cujos cafés são de má qualidade. A hibridação interespecífica, que poderia reunir em uma só planta caraterísticos de rusticidade e boa qualidade de bebida, tem o inconveniente de dar origem a plantas triplóides, que são estéreis. Daí se deduz que a obtenção artificial de formas que combinassem êsses caraterísticos constitui problema, cuja solução é extremamente demorada. No presente trabalho, apresentam-se os caracteres de uma nova forma de Coffea, encontrada em cafèzal da Fazenda Itaporã, em Terra Roxa, município de Viradouro, que, com algumas ressalvas, oferece a desejada combinação de caracteres. Trata-se, provàvelmente, de um híbrido espontâneo entre C. arabica e C. Dewevrei, com 2n = 44 cromosômios, extremamente rústico e produtivo, cujas sementes fornecem uma bebida que pode ser classificada como boa. Apenas apresenta, como principal defeito, uma auto-esterilidade quase completa. Os seus caraterísticos botânicos são descritos em detalhe. Devido ao seu porte elevado, ramos abundantes e folhas grandes e coriáceas, esse cafeeiro se assemelha ao C. Dewevrei. Os frutos são oval-elípticos, de um vermelho bem escuro quando maduros, e as sementes oblongas, constatan-do-se elevada percentagem do tipo "moca" e "chocha". Quanto à constituição citológica, as pesquisas conduziram à hipótese de este cafeeiro possuir 22 cromosômios de C. arabica e 22 (n

  5. Applications of hydrogen peroxide in electrochemical technology

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez Gallegos, Alberto Armando

    1998-12-01

    oxigeno (COD) fue agotada a niveles por arriba del 85%, indicando una mineralizacion completa de los agentes contaminantes organicos. La vida promedio de del catodo vitreo reticulado de carbon se demostro que era mayor que las 1000 horas durante dos y medio anos de experimentos. Durante este tiempo el funcionamiento del catodo fue muy bueno, conduciendo a resultados reproducibles e indicando que bajo las condiciones experimentales estudiadas el catodo de carbon vitreo reticulado y los componentes de la celula eran quimicamente y electroquimicamente estables.

  6. A new model for commercially sustainable renewable energy-based rural electrification in Indonesia

    Energy Technology Data Exchange (ETDEWEB)

    Walt, Robb [Integrated Power Corporation-Indonesia, (United states)

    1995-12-31

    ] Las rapidas crecientes demandas y los requerimientos de acceso a la electricidad por todas las areas remotas de Indonesia juntamente con los subsidios de mas de $500 millones de dolares para la electrificacion rural han forzado al gobierno de Indonesia a buscar alternativas al modelo convencional de empresa de servicios electricos para la electrificacion rural. En 1992-1993 se llevo a cabo un estudio en colaboracion con la Agencia para la Aplicacion y la Evaluacion de Tecnologia (BPPT)del Gobierno de Indonesia y la Empresa Nacional de Energia Electrica (PLN) para apoyar la busqueda de soluciones sustentables para la electrificacion de comunidades remotas. Este estudio produjo un Nuevo Modelo comercial para la electrificacion de comunidades rurales fuera de la red en Indonesia con servicios de la calidad de empresa electrica. Este nuevo modelo esta caracterizado por el uso de nuevas tecnologias para la generacion, distribucion y ventas de electricidad. La clave del exito del nuevo modelo son las plantas electricas hibridas basadas en energia renovable y en el uso de medidores flexibles suministradores de energia bajo demanda. Las tarifas estimadas para el servicio de electricidad estan basadas en las cantidades que actualmente se pagan por los propietarios de casas rurales por keroseno, velas y servicios de baterias a diferentes niveles de ingreso. El estudio mostro que la mayor parte de los propietarios de casa estan deseosos y tienen la capacidad para pagar cantidades adicionales por valiosos servicios confiables de electricidad del grado de empresa de servicios electricos, tales como mejor alumbrado, entretenimiento de television y para usos productivos (economicos) durante las horas diurnas. Se llevo a cabo una evaluacion financiera en sistemas hibridos de energia electrica en comunidades fuera de la red con ganancias generadas en base a las tarifas del mercado y cobradas mediante nueva tecnologia para la compra de electricidad y pago anticipado en base a un

  7. ¿Hilo de Ariadna o tela de Penélope? Una reflexión crítica sobre la política de vivienda urbana para la población en situación de desplazamiento en Colombia. Ariadne’s thread or Penelope’s shroud? A critical reflection about the urban housing policy for the displaced population in Colombia. Fio de Ariadna ou tecido de Penélope? Uma reflexão crítica sobre a política da vivenda urbana para a população em situação de deslocamento na Colômbia.

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    Jean François Jolly

    2008-06-01

    of the “State in action” and manner of “solving problems.” The article then, reviews the housing public policy for displaced populations, and compares it with the problem it is trying to solve. With this information, it will be possible to assert that the character of the policies is centralist and assistentialist with limited and controlled territoriality. On the other hand, the VIS urban public policy does not allow to think about solving the problems of the displaced population due to violence. The article proposes general principles for a comprehensive housing public policy for displaced populations. Resumo O alto grau de vulnerabilidade da população deslocada e o baixo impacto dos programas dirigidos a essa população, tem trazido como resultado a resolução de problemáticas de vivenda, baixo condições de ilegalidade, originadas pela criação de bairros com baixa qualidade de vivenda em termos de habitabilidade. Em vês de propor uma avaliação da política da vivenda para deslocados pela violência, o artigo propõe uma análise da política pública de vivenda para deslocados. Nesta perspectiva, depois de apresentar a análise das políticas públicas como análise do “Estado em ação” e maneira de “resolver problemas,” se revisa a política pública de vivenda para a população deslocada. Também se compara com o problema que pretende resolver, pelo qual será possível afirmar que o caráter da política é centralista e assistencialista com territorialidade limitada e controlada. Do outro lado, a política pública da VIS urbana se caracteriza como uma política dual e hibrida, caso típico da “sec-territorialidade” e da “governança.” Considerando que as lógicas respectivas das duas políticas públicas não permitam pensar na resolução dos problemas de vivenda da população deslocada, o artigo propõe princípios gerais de uma política pública de vivenda.

  8. Neuralfussy multivariable control applied to the control of velocity, power, and exhaust gas temperature of a turbo gas unit; Control neurodifuso multivariable aplicado al control de velocidad, potencia y temperatura de gases de escape de una unidad turbogas

    Energy Technology Data Exchange (ETDEWEB)

    Segura Ozuna, Victor Octavio

    2004-11-15

    construccion mas cortos, a bajos niveles de emision de contaminantes y costos de operacion competitivos. El sistema de control de las unidades turbogas esta basado en algoritmos de control convencionales del tipo PI [2]. Este esquema de control es destinado para tareas de regulacion y rechazo a perturbaciones, y no para seguimiento de puntos de referencia. Los controladores actuan todos sobre una sola valvula de control, lo cual representa una fuerte interaccion entre los mismos, por ejemplo un ajuste en los parametros del algoritmo del PI digital de temperatura, puede mejorar su desempeno pero tambien puede afectar el desempeno del control de velocidad o el de potencia. La turbina de gas presenta un comportamiento no lineal y variante en el tiempo, principalmente en la etapa de arranque donde se presentan varios disturbios importantes. Actualmente, los controladores utilizados en los esquemas de control de las turbinas son lineales, los cuales son sintonizados para un punto de operacion especifico y son conservados asi por tiempo indefinido. En esta tesis se presenta la formulacion de un controlador prealimentado multivariable, disenado con la combinacion de las tecnologias de logica difusa y redes neuronales con el proposito de mejorar el control de velocidad, potencia y temperatura de la UTG. Este control propuesto es usado en conjuncion con el esquema de control convencional de la UTG existente, para integrar una estrategia de control hibrida prealimentada. El control prealimentado esta compuesto por un sistema de inferencia difuso de multiples entradas y una sola salida disenado con datos de entrada y salida de la planta. El controlador retroalimentado esta compuesto por controladores tipo PI convencionales en este tipo de unidades. Con esta estrategia, el controlador prealimentado provee una mayor contribucion en la senal de control disminuyendo el esfuerzo de control de los controladores PI convencionales y la fuerte interaccion que existe entre ellos. Los controladores

  9. Melhoramento do cafeeiro: XLI. Produtividade do híbrido de timor, de seus derivados e de outras fontes de resistência a Hemileia vastatrix Coffee breeding: XLI. Yield of 'híbrido de timor' progenies, its derivatives and other sources of resistance to Hemileia vastatrix

    Directory of Open Access Journals (Sweden)

    Alcides Carvalho

    1989-01-01

    Full Text Available Progênies do café Híbrido de Timor e F02-F4 oriundas de cruzamentos desse café com outros cultivares resistentes ou não a Hemileia vastatrix e cruzamentos entre outras fontes de resistência ao patógeno, foram avaliadas em três experimentos, em Campinas, para observação de sua produtividade, em relação a alguns cultivares de Coffea arabica tomados como testemunhas. As progênies do Híbrido de Timor apresentaram pequena produtividade, indicando baixa adaptação, com exceção daquelas de prefixos C 1737, C 1738 e C 1699. As progênies derivadas de cruzamentos do Híbrido de Timor com cultivares de porte pequeno, como Caturra Vermelho e Vila Sarchi de Coffea arabica, mostraram-se, também, pouco produtivas. Destacou-se apenas a progênie C 1669, rústica. Das combinações do Híbrido de Timor com outros cultivares de C. arabica com resistência a H. vastatrix, apenas a progênie C 1698 se revelou melhor. As progênies F2 derivadas de cruzamentos do cultivar S 795 portador do fator S H3 de resistência com Mundo Novo, deram produções bastante razoáveis. Notou-se, de modo geral, acentuada variabilidade na produção das progênies, o que é indicado pelos elevados valores dos coeficientes de variação obtidos nos três experimentos. Os dados desses experimentos mostraram a dificuldade de aproveitamento das progênies e dos derivados do Híbrido de Timor analisados. Tratando-se, no entanto, de material de elevado grau de resistência às raças de H. vastatrix, novas hibridações deverão ser sintetizadas, com cultivares comerciais, a fim de se conseguirem linhagens resistentes, vigorosas e mais produtivas.'Híbrido de Timor' coffee is probably derived from a natural cross between Coffea arabica and C. canephora. Selections carried out at the "Centro de Investigação das Ferrugens do Cafeeiro", Portugal, resulted in the isolation of tetraploid self-compatible coffee plants, like CIFC 832/1 and 832/2, with high resistance to coffee

  10. Deleção 22q11.2 em pacientes com defeito cardíaco conotruncal e fenótipo da síndrome da deleção 22q11.2 Deleción 22q11.2 en pacientes con defecto cardiaco conotruncal y fenotipo del síndrome de la deleción 22q11.2 22q11.2 deletion in patients with conotruncal heart defect and del22q syndrome phenotype

    Directory of Open Access Journals (Sweden)

    Sintia Iole Nogueira Belangero

    2009-04-01

    Full Text Available FUNDAMENTO: A síndrome da deleção 22q11.2 é a mais freqüente síndrome de microdeleção humana. O fenótipo é altamente variável e caracterizado por defeito cardíaco conotruncal, dismorfias faciais, insuficiência velofaríngea, dificuldade de aprendizagem e retardo mental. OBJETIVO: O objetivo deste trabalho foi investigar a freqüência da deleção 22q11.2 em uma amostra brasileira de indivíduos portadores de cardiopatia conontrucal isolada e do fenótipo da síndrome da deleção 22q11.2. MÉTODOS: Vinte e nove pacientes foram estudados por meio de citogenética clássica, por hibridação in situ fluorescente (FISH e por técnicas moleculares. RESULTADOS: A análise citogenética por meio de bandamento G revelou cariótipo normal em todos os pacientes, com exceção de um que apresentou cariótipo 47,XX,+idic(22(q11.2. Com o uso de técnicas moleculares, a deleção foi observada em 25% dos pacientes, todos portadores do fenótipo da síndrome da deleção 22q11.2. Em nenhum dos casos, a deleção foi herdada dos pais. A freqüência da deleção 22q11.2 foi maior no grupo de pacientes portadores do espectro clínico da síndrome da deleção 22q11.2 do que no grupo de pacientes com cardiopatia conotruncal isolada. CONCLUSÃO: A investigação da presença da deleção e sua correlação com os dados clínicos dos pacientes podem auxiliar os pacientes e suas famílias a terem um melhor aconselhamento genético e um seguimento clínico mais adequado.FUNDAMENTO: El síndrome de la deleción 22q11.2 es el más frecuente síndrome de microdeleción humana. El fenotipo, altamente variable, se caracteriza por defecto cardiaco conotruncal, dismorfias faciales, insuficiencia velofaríngea, dificultad de aprendizaje y retardo mental. OBJETIVO: El objetivo de este trabajo fue investigar la frecuencia tanto de la deleción 22q11.2 en una muestra brasileña de individuos portadores de cardiopatía conotrucal aislada, como del fenotipo del s

  11. Reliability optimization in compound electric power systems with the aid of evolutionary algorithms; Optimizacion de la confiabilidad en sistemas electricos de potencia compuestos utilizando algoritmos evolucionarios

    Energy Technology Data Exchange (ETDEWEB)

    Gomez Hernandez, Jose Alberto

    2001-11-15

    and branches, that contributes the most to the voltage collapse or singular point. The obtained results show the feasibility of the proposed algorithm for optimization of reliability considering conditions of security. The tested systems used are: Reliability test system of 24 nodes of the IEEE, Roy Billinton test system of 6 nodes and eastern area equivalent of the Mexican grid of 31 nodes of the Comision Federal de Electricidad. [Spanish] El proposito de evaluar la confiabilidad de Sistemas Electricos de Potencia (SEP) es para estimar la habilidad del sistema para desempenar su funcion de llevar la energia de las estaciones generadoras a los puntos de carga. Esto involucra la confiabilidad de fuentes de generacion y medios de transmision que afecta en la transferencia de potencia a traves del sistema de transmision que conlleva a perdida de carga y caidas de voltaje entre la generacion y los centros de consumo. En esta tesis se desarrolla una metodologia hibrida que optimiza la confiabilidad en sistemas compuestos (generacion-transmision) utilizando algoritmos evolucionarios. Esta tecnica de optimizacion determina el numero optimo de componentes (redundancia paralela en lineas de transmision) y asignacion de compensacion en derivacion en nodos de carga de la red necesarios para maximizar la confiabilidad sujeta a restricciones de costo, considerando condiciones de seguridad en estado estacionario usando la tecnica del minimo valor singular (MVS) como indicador a la inestabilidad de voltaje. La funcion objetivo se define como una funcion estocastica, donde la medida de interes es el MVS de la matriz Jacobiana de flujos de potencia del evento mas severo de acuerdo a la evaluacion de confiabilidad del sistema compuesto, esta formulacion es una combinacion de programacion no lineal entera y continua, donde los algoritmos convencionales de programacion matematica presentan dificultades en robustez y en la busqueda del optimo global. Los eventos de falla de las unidades

  12. Produção e qualidade da moranga híbrida em resposta a doses de nitrogênio Yield and quality of hybrid squash (pumpkin in response to nitrogen doses

    Directory of Open Access Journals (Sweden)

    Marinalva W Pedrosa

    2012-06-01

    Full Text Available Foram avaliados os efeitos de doses de N na produção e qualidade da moranga hibrida cv. Tetsukabuto (Takaima F1 em experimento na EPAMIG Centro Oeste, Prudente de Morais (MG, de 25/08 a 03/12/2008. Foram avaliadas cinco doses de nitrogênio (0; 37,5; 75; 150; 300 kg ha-1, na forma de uréia, divididas em quatro aplicações (30% no plantio, 20% aos 20 dias, 30% aos 40 dias e 20% aos 60 dias após a emergência, num delineamento experimental de blocos casualizados com quatro repetições. As doses de N influenciaram significativamente as características avaliadas. O número de frutos aumentou de 2.498 ao ponto de máximo de 6.794 frutos ha-1 com a aplicação de 219 kg ha-1 de N. O diâmetro e a espessura da polpa do fruto aumentaram de 14,97 e 2,33 cm, até atingirem os pontos máximos de 17,74 e 2,80 cm nas doses de 171 e 128 kg ha-1 de N, respectivamente. O teor de sólidos solúveis na polpa de frutos de moranga híbrida aumentou com o aumento das doses de N observando-se com a aplicação de 300 kg ha-1 de N teor de 8ºBrix. A produtividade de frutos foi também influenciada pelas doses de N estimando-se como valor máximo 11,55 t ha-1 de frutos com a aplicação de 300 kg ha-1 de N. A dose estimada de N relativa à máxima eficiência econômica foi obtida com a aplicação de 262 kg ha-1, considerando os preços de R$ 2,41 kg-1 de N e de R$ 560,00 t-1 de moranga.An experiment was carried out at EPAMIG Centro Oeste, Prudente de Morais, Minas Gerais state, Brazil, from August 25 to December 2, 2008, to evaluate the effects of N rates on production and quality of hybrid squash cv. Tetsukabuto (Takaima F1. The treatments were five N rates (0, 37.5, 75, 150, 300 kg ha-1, as urea, split in four applications (30% at planting date, 20% at 20 days, 30% at 40 days and 20% at 60 days after emergence in a randomized complete block design, with four replications. The N rates significantly affected the characteristics evaluated. The number of fruits

  13. Comparação entre métodos para testar a viabilidade de pólen de pessegueiro Comparison among methods for testing pollen viability

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    Patrícia Milech Einhardt

    2006-04-01

    Full Text Available Para o sucesso das hibridações controladas, é importante que o pólen a ser utilizado tenha boa viabilidade (> 30%. Os métodos para testá-la podem ser classificados em quatro tipos: 1- uso de corantes; 2-germinação in vitro; 3- germinação in vivo; 4- porcentagem de frutificação efetiva, obtida com a utilização do pólen em teste. No presente trabalho, realizado em agosto de 2004, fez-se uma comparação entre os três primeiros métodos citados acima, utilizando-se de amostras de pólen de pessegueiro, coletadas em 2003, de cinco cultivares: Esmeralda, Eldorado, Granada, Maciel e Vanguarda. O meio de cultura utilizado para a germinação in vitro foi constituído de 10g de açúcar cristal e 1g de ágar para 100ml de água destilada. Para a germinação in vivo foram emasculadas e polinizadas, em laboratório, flores de ramos coletados no campo e mantidos em frascos com água. Seguiu-se a metodologia utilizada em microscópio de rotina, com corante diferencial a fim de observar os tubos polínicos no estigma ou pistilo. No método de coloração, foi utilizado carmim como corante. Os resultados da análise de variância mostraram que as diferenças entre cultivares, entre métodos e a interação cultivar e método foram altamente significativas. O método de coloração, usando carmin propiônico como corante, foi significativamente superior à germinação in vivo, em todas as cultivares testadas. A germinação in vitro propiciou resultado estatisticamente igual à germinação in vivo, com exceção das cultivares Esmeralda e Granada, nas quais a germinação in vitro foi superior. Entretanto, a maior diferença entre os dois métodos foi de 10,03% na cultivar Esmeralda que, embora estatisticamente significativa, na prática, seria aceitável em testes de rotina. Concluiu-se que o teste in vitro é representativo da situação in vivo, enquanto o método de coloração superestimou a porcentagem de pólen viável.It is important

  14. Predictive factors of breast cancer evaluated by immunohistochemistry Fatores preditivos do câncer de mama avaliados pela imuno-histoqu��mica

    Directory of Open Access Journals (Sweden)

    Helenice Gobbi

    2008-04-01

    melhorar os resultados da IHQ, porém não recupera tecidos com autólise ou com excessiva fixação. A escolha do anticorpo primário para a IHQ, considerando sua sensibilidade e sua especificidade de acordo com a resposta terapêutica, representa uma importante etapa. Além de anticorpos monoclonais de camundongo, novos anticorpos monoclonais de coelho são comercialmente disponíveis, tais como clones SP1 e B644 anti-RE, SP2 e B645 anti-RP, e SP3 e 4B5 anti-Her2. Eles representam uma alternativa para avaliação de receptores hormonais e Her2 através da IHQ. Novos sistemas de detecção poliméricos não-biotinilados também são disponíveis e permitem marcação exata e forte sem marcação estromal ou citoplasmática inespecífica devido à biotina endógena. O cut off mais recomendado para receptor de estrogênio (RE e receptor de progesterona (RP é acima de 1% de células positivas com marcação moderada ou forte (sistema de escore de Allred. Novas recomendações para avaliação de Her2 através da IHQ apontam um cut off de mais de 30% de células positivas com marcação forte (3+, que melhor se relaciona com amplificação gênica. Os casos 2+ são agora considerados indeterminados e devem ser confirmados por hibridação in situ por fluorescência (FISH ou hibridização in situ colorimétrica (CISH. Um controle de qualidade de fases pré-analítica, analítica e pós-analítica da IHQ é recomendado para a otimização dos resultados.

  15. Genética de Coffea XXIV - Mutantes de Coffea arabica procedemtes da Etiópia Genetics of Coffea XXIV - Mutants of Coffea arabica from ethiopia

    Directory of Open Access Journals (Sweden)

    Alcides Carvalho

    1959-01-01

    Full Text Available Vários conjuntos de mudas de café da Etiópia foram recebidos em Campinas em 1952 e 1953, através do Departamento de Agricultura dos Estados Unidos da América do Norte. Entre êsses cafeeiros foram observadas diversas variações, as quais vêm sendo analisadas sob os aspectos morfológico e genético. Verificou-se que o café recebido com a denominação de Eritrean Moca não é um híbrido da variedade mokka de Coffea arabica, mas intientifica-se com o mutante semper-florens e é homozigoto para o par de alelos sfsf. Pelas hibridações realizadas com a variedade murta notou-se que o alelo t, que caracteriza o café Bourbon, é freqüente no material provindo da Etiópia, embora também af ocorram plantas da variedade typica de constituição TTNaNa. Apesar de ainda não se terem identificado os alelos responsáveis pela variedade abyssinica, observou-se que alguns lotes de mudas são típicos dessa variedade e trazem os alelos TT, enquanto em outros nota-se segregação para plantas abyssinica menos características e estas, com freqüência, trazem os alelos tt. Quanto à côr dos brotos, pode-se concluir que o alelo br ocorre com bastante freqüência no material recebido e o alelo Br, em escala menor. Um novo alelo semi-erecta, recessivo, foi encontrado em plantas da Etiópia. Nos mesmos conjuntos também ocorrem plantas com tipo de ramificação semi-erecta não muito característica, provavelmente controlada por outros fatores genéticos. Observaram-se também cafeeiros com frutos e sementes grandes (macrocarpa nada se sabendo, todavia, a respeito de sua constituição genética. Plantas com cálice pouco desenvolvido foram notadas mais freqüentemente nos lotes de café Agaro e Cioiccie. Êste característico não è condicionado pelo alelo sd, da variedade goiaba, tendo-se apenas verificado uma interação entre os dois fatôres responsáveis pelo desenvolvimento foliar do cálice. Alguns conjuntos encerram plantas resistentes

  16. Melhoramento do cafeeiro XV: variabilidade observada em progênies de café Coffee breeding XV: variability observed in coffee progenies

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    A. Carvalho

    1959-01-01

    Full Text Available As análises das produções de cafeeiros constituintes de diversas progênies, principalmente de café Bourbon Vermelho, com produções controladas por 12 a 15 anos. mostraram acentuada variabilidade na produção, o mesmo ocorrendo entre cafeeiros F1 derivados de hibridações entre plantas selecionadas. A fim de avaliar as causas dessa variação foram escolhidas, em quatro progenies S1 e em três híbridos F1, cafeeiros com as mais altas e as mais baixas produções, bem como uma planta em cada progênie com produção correspondente à classe mediana, a fim de servirem de plantas matrizes para derivação de progênies correspondentes às gerações S2 e F2. Estas foram plantadas, em 1953, em um ensaio comparativo, onde também foram incluídas novas progenies S1 e F1 dos mesmos cafeeiros, bem como progênies selecionadas de outras variedades comerciais como Bourbon Amarelo e Mundo Novo. A análise dos resultados das quatro primeiras produções (1955 a 1958 bem como da altura e vigar dos cafeeiros indicou, em todos os conjuntos de progênies examinadas, que a grande variação observada entre as plantas de uma mesma progênie de Bourbon é principalmente devida a influências do ambiente, desde que não se notaram diferenças na produção das progênies S2 e F2 derivadas de plantas S1 e F1 com as mais altas e as mais baixas produções. A mesma condição se verificou em relação à altura das plantas. Os valores das variâncias da produção para as progênies S1 e S2 em cada grupo de progenies não indicaram nenhum efeito específico da autofecundação, reduzindo-os ou aumentando-os. Nas gerações F2 as variâncias se mostraram mais uniformes, porém as diferenças entre as variâncias das gerações F1 e F2 não foram significativas. Pode-se concluir dos dados dêste ensaio que a seleção do café Bourbon Vermelho deve ser baseada na produção média das progenies e que a seleção dentro da progênie não conduz a nenhum

  17. Study of multilevel inverters with extended functions; Estudio de inversores multinivel con funciones ampliadas

    Energy Technology Data Exchange (ETDEWEB)

    Miranda Vidales, Homero

    2007-06-15

    pueden conmutar a muy baja frecuencia entregando a la salida una forma de onda con muy baja distorsion armonica. Un filtro activo de potencia esta disenado para la compensacion de armonicas, regulacion, etcetera. Por lo tanto es necesario realizar un estudio que permita extender las funciones del filtro activo de tal manera que sea un equipo capaz de realizar otro tipo de funciones. En este trabajo se estudia al convertidor multinivel con celdas en cascada para funciones extendidas. En donde la funcion principal esta en la etapa de potencia del filtro activo de corriente. Ademas, se estudian dos posibilidades que permitan extender su funcionamiento como un STATCOM y un filtro con capacidad regenerativa. El estudio implica proponer uno o varios esquemas de control para cada una de las funciones ya sea de manera independiente o en conjunto. El capitulo II presenta un analisis de la topologia hibrida asimetrica con relacion 3:1 en las fuentes de CD. Este convertidor se utiliza en la etapa de potencia del filtro activo. Tambien se propone un esquema para obtener la corriente de referencia. En este capitulo se aborda el diseno de dos esquemas de control los cuales estan basados en la teoria del control lineal y no lineal. En el Capitulo III se estudian las funciones ampliadas del convertidor multinivel. Primero se presenta un estudio sobre la funcion como STATCOM. Para esta funcion se propone un esquema de control el cual permite realizar la funcion de filtro activo y STATCOM a la vez. En la segunda parte se estudia el inversor multinivel con capacidad de regeneracion. En esta parte del capitulo se realiza una modificacion de la estructura que ayuda a desempenar esta funcion. El capitulo IV contiene las discusiones y conclusiones de los resultados obtenidos en la tesis. En la ultima parte del capitulo se comentan algunas lineas de investigacion futuras y finalmente se muestran los articulos derivados de esta tesis. Se incluyen tambien seis apendices que complementan el

  18. Une madone à Fukushima. La condition numérique du portrait de presse

    Directory of Open Access Journals (Sweden)

    Adeline Wrona

    2014-04-01

    escritório encarregado pela produção de informações na Ásia, a identidade do fotógrafo. Todos esses detalhes estão arquivados na página de Facebook consagrada à imagem. Assim, a hibridação dos padrões visuais asiáticos e ocidentais se combina com uma mutação das formas jornalísticas, sob a égide das mídias informatizadas.

  19. Usos de Twitter durante el 15M. El caso de la prensa catalana

    Directory of Open Access Journals (Sweden)

    Juan Linares-Lanzman

    2015-03-01

    de rédaction. Durante as primeiras manifestações do 15M ou movimento dos indignados,milhares de usuários espanhóis se conectaram ao Twitter na medida em quese multiplicam as informações sobre o movimento neste meio. Estas serviram para o 15M organizar e difundir, muitas vezes em tempo real, assuntos relevantes sobre as manifestações. Este artigo é um estudo de caso que mostra alguns usos jornalísticos do Twitter nesse período, aplicado ao caso específico do 15M na Catalunha, já que Barcelona (a Acampada de Barcelona foi um dos principais acampamentos do movimento. O objetivo é explorar como foram as rotinas das redações jornalísticas no Twitter, e se foi produzida e como, uma hibridação entre as funções de jornalistas e usuários da ferramenta. Realizamos entrevistas com jornalistas das principais redações catalãs e combinamos com uma análise de conteúdo de alguns tweets publicados entre os dias 15 de maio e 19 de junho de 2011, período conhecido como o da “explosão do movimento”. Os resultados deste artigo sugerem que, motivados por uma grande necessidade por informações sobre o que estava acontecendo, os jornalistas optaram por buscar no Twitter novas fontes, especialmente da rede do 15M, constituída por nós dos acampamentos e da Democracia Real Ya, a plataforma responsável pelas manifestações do 15 de maio de 2011. No decorrer do 15M se instalou uma rotina jornalística que superou a lógica de simplesmente acessar e dar crédito a fontes de informação previamente reconhecidas, como as agências de notícias e o outras mídias, mas foi utilizada uma nova fórmula, que permitiu aos jornalistas dar mais amplitude e profundidade às notícias e informações sobre o 15M. Este fato leva à necessidade de se pensaros modelos do jornalismo do tipo participativo, em que as mídias sociais e algumas funções jornalísticas tradicionais podem ser exercidas por outros usuários, incluindo desconhecidos, que atuam fora das redações.