WorldWideScience

Sample records for cell demonstration program

  1. USEPA Arsenic Demonstration Program

    Science.gov (United States)

    The presentation provides background information on the USEPA arsenic removal program. The summary includes information on the history of the program, sites and technology selected, and a summary of the data collected from two completed projects.

  2. Overview about the fuel cell bus demonstration programs CUTE, ECTOS and STEP

    International Nuclear Information System (INIS)

    'Full text:' The paper will give an overview about the CUTE, ECTOS and STEP projects. The aim of the projects is to develop and demonstrate a emission-free and low-noise transport system, including the accompanying energy infrastructure, which has great potential for reducing the global greenhouse effect according to the Kyoto protocol, improving the quality of the atmosphere and life in densely populated areas and conserving fossil resources. For this purpose the application of the innovative hydrogen-based fuel cell technology is established by using fuel cell powered buses in an urban environment together with novel hydrogen production and support systems as part of a European Union wide demonstration scheme. The project demonstrates also to European Society the availability of the FC technology as a safe and reliable transportation technology. The major objectives are as follows: Demonstration of more than 20 fuel cell powered regular service buses over a period of two years in several European inner city areas to illustrate the different operating conditions to be found in Europe; Design, construction and operation of the necessary infrastructure for hydrogen production, including the required refuelling stations; Collection of findings concerning the construction and operating behaviour of hydrogen production for mobile use, and exchange of experiences including bus operation under differing conditions among the numerous participating companies; and, the research work of IKP and PE comprises the ecological analysis of the entire life cycle and comparison with conventional alternatives (diesel driven buses, CNG-buses). It also includes the economical analysis of the hydrogen infrastructure. First experiences from CUTE and ECTOS were presented. (author)

  3. Electric thermal storage demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    1992-02-01

    In early 1989, MMWEC, a joint action agency comprised of 30 municipal light departments in Massachusetts and on affiliate in Rhode Island, responded to a DOE request to proposal for the Least Cost Utility Planning program. The MMWEC submission was for the development of a program, focused on small rural electric utilities, to promote the use of electric thermal storage heating systems in residential applications. This report discusses the demonstration of ETS equipment at four member light departments.

  4. Electric thermal storage demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    In early 1989, MMWEC, a joint action agency comprised of 30 municipal light departments in Massachusetts and on affiliate in Rhode Island, responded to a DOE request to proposal for the Least Cost Utility Planning program. The MMWEC submission was for the development of a program, focused on small rural electric utilities, to promote the use of electric thermal storage heating systems in residential applications. This report discusses the demonstration of ETS equipment at four member light departments.

  5. Propane Vehicle Demonstration Grant Program

    Energy Technology Data Exchange (ETDEWEB)

    Jack Mallinger

    2004-08-27

    Project Description: Propane Vehicle Demonstration Grants The Propane Vehicle Demonstration Grants was established to demonstrate the benefits of new propane equipment. The US Department of Energy, the Propane Education & Research Council (PERC) and the Propane Vehicle Council (PVC) partnered in this program. The project impacted ten different states, 179 vehicles, and 15 new propane fueling facilities. Based on estimates provided, this project generated a minimum of 1,441,000 new gallons of propane sold for the vehicle market annually. Additionally, two new off-road engines were brought to the market. Projects originally funded under this project were the City of Portland, Colorado, Kansas City, Impco Technologies, Jasper Engines, Maricopa County, New Jersey State, Port of Houston, Salt Lake City Newspaper, Suburban Propane, Mutual Liquid Propane and Ted Johnson.

  6. The MAJORANA Demonstrator Radioassay Program

    Energy Technology Data Exchange (ETDEWEB)

    Abgrall, N.; Arnquist, Isaac J.; Avignone, F. T.; Back, Henning O.; Barabash, Alexander S.; Bertrand, F.; Boswell, M.; Bradley, A. W.; Brudanin, V.; Busch, Matthew; Buuck, M.; Byram, D.; Caldwell, A. S.; Chan, Yuen-Dat; Christofferson, C. D.; Chu, P. H.; Cuesta, C.; Detwiler, Jason A.; Dunmore, J. A.; Efremenko, Yuri; Ejiri, H.; Elliott, S.; Finnerty, P.; Galindo-Uribarri, A.; Gehman, Victor M.; Gilliss, T.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guinn, I.; Guiseppe, V. E.; Henning, R.; Hoppe, Eric W.; Howard, S.; Howe, M. A.; Jasinski, B. R.; Johnson, R. A.; Keeter, K.; Kidd, M. F.; Kochetov, Oleg; Konovalov, S.; Kouzes, Richard T.; Laferriere, Brian D.; Leon, Jonathan D.; Loach, J. C.; MacMullin, J.; MacMullin, S.; Martin, R. D.; Massarcyk, R.; Meijer, S. J.; Mertens, S.; Miller, M. L.; Orrell, John L.; O' Shaughnessy, C.; Overman, Nicole R.; Poon, Alan W.; Pushkin, K.; Radford, D. C.; Rager, J.; Rielage, Keith; Robertson, R. G. H.; Romero-Romero, E.; Ronquest, M. C.; Schubert, Alexis G.; Shanks, B.; Shirchenko, M.; Snavely, Kyle J.; Snyder, N.; Steele, David; Suriano, Anne-Marie; Tedeschi, D.; Trimble, J. E.; Varner, R. L.; Vasilyev, Sergey; Vetter, K.; Vorren, Kris R.; White, B. R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Yu, Chang-Hong; Yumatov, Vladimir; Zhitnikov, I.

    2016-05-03

    The Majorana collaboration is constructing the Majorana Demonstrator at the Sanford Underground Research Facility at the Homestake gold mine, in Lead, SD. The apparatus will use Ge detectors, enriched in isotope 76Ge, to demonstrate the feasibility of a large-scale Ge detector experiment to search for neutrinoless double beta decay. The long half-life of this postulated process requires that the apparatus be extremely low in radioactive isotopes whose decays may produce backgrounds to the search. The radioassay program conducted by the collaboration to ensure that the materials comprising the apparatus are suffciently pure is described. The resulting measurements of the radioactiveisotope contamination for a number of materials studied for use in the detector are reported.

  7. The MAJORANA DEMONSTRATOR radioassay program

    Science.gov (United States)

    Abgrall, N.; Arnquist, I. J.; Avignone, F. T.; Back, H. O.; Barabash, A. S.; Bertrand, F. E.; Boswell, M.; Bradley, A. W.; Brudanin, V.; Busch, M.; Buuck, M.; Byram, D.; Caldwell, A. S.; Chan, Y.-D.; Christofferson, C. D.; Chu, P.-H.; Cuesta, C.; Detwiler, J. A.; Dunmore, J. A.; Efremenko, Yu.; Ejiri, H.; Elliott, S. R.; Finnerty, P.; Galindo-Uribarri, A.; Gehman, V. M.; Gilliss, T.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guinn, I. S.; Guiseppe, V. E.; Henning, R.; Hoppe, E. W.; Howard, S.; Howe, M. A.; Jasinski, B. R.; Johnson, R. A.; Keeter, K. J.; Kidd, M. F.; Kochetov, O.; Konovalov, S. I.; Kouzes, R. T.; LaFerriere, B. D.; Leon, J.; Loach, J. C.; MacMullin, J.; MacMullin, S.; Martin, R. D.; Massarczyk, R.; Meijer, S.; Mertens, S.; Miller, M. L.; Orrell, J. L.; O'Shaughnessy, C.; Overman, N. R.; Poon, A. W. P.; Pushkin, K.; Radford, D. C.; Rager, J.; Rielage, K.; Robertson, R. G. H.; Romero-Romero, E.; Ronquest, M. C.; Schubert, A. G.; Shanks, B.; Shirchenko, M.; Snavely, K. J.; Snyder, N.; Steele, D.; Suriano, A. M.; Tedeschi, D.; Trimble, J. E.; Varner, R. L.; Vasilyev, S.; Vetter, K.; Vorren, K.; White, B. R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Yu, C.-H.; Yumatov, V.; Zhitnikov, I.

    2016-08-01

    The MAJORANA collaboration is constructing the MAJORANA DEMONSTRATOR at the Sanford Underground Research Facility at the Homestake gold mine, in Lead, SD. The apparatus will use Ge detectors, enriched in isotope 76Ge, to demonstrate the feasibility of a large-scale Ge detector experiment to search for neutrinoless double beta decay. The long half-life of this postulated process requires that the apparatus be extremely low in radioactive isotopes whose decays may produce backgrounds to the search. The radioassay program conducted by the collaboration to ensure that the materials comprising the apparatus are sufficiently pure is described. The resulting measurements from gamma-ray counting, neutron activation and mass spectroscopy of the radioactive-isotope contamination for the materials studied for use in the detector are reported. We interpret these numbers in the context of the expected background for the experiment.

  8. 76 FR 79193 - Medicare Program; Independence at Home Demonstration Program

    Science.gov (United States)

    2011-12-21

    ... HUMAN SERVICES Centers for Medicare & Medicaid Services Medicare Program; Independence at Home Demonstration Program AGENCY: Centers for Medicare & Medicaid Services (CMS), HHS. ACTION: Notice. SUMMARY: This notice creates a new demonstration program for chronically ill Medicare beneficiaries to test a...

  9. Clean Coal Technology Demonstration Program: Program Update 2000

    Energy Technology Data Exchange (ETDEWEB)

    Assistant Secretary for Fossil Energy

    2001-04-01

    Annual report on the Clean Coal Technology Demonstration Program (CCT Program). The report address the role of the CCT Program, implementation, funding and costs, accomplishments, project descriptions, legislative history, program history, environmental aspects, and project contacts. The project descriptions describe the technology and provides a brief summary of the demonstration results.

  10. Clean Coal Technology Demonstration Program: Program Update 2001

    Energy Technology Data Exchange (ETDEWEB)

    Assistant Secretary for Fossil Energy

    2002-07-30

    Annual report on the Clean Coal Technology Demonstration Program (CCT Program). The report address the role of the CCT Program, implementation, funding and costs, accomplishments, project descriptions, legislative history, program history, environmental aspects, and project contacts. The project descriptions describe the technology and provides a brief summary of the demonstration results. Also includes Power Plant Improvement Initiative Projects.

  11. Clean Coal Technology Demonstration Program: Program Update 1998

    Energy Technology Data Exchange (ETDEWEB)

    Assistant Secretary for Fossil Energy

    1999-03-01

    Annual report on the Clean Coal Technology Demonstration Program (CCT Program). The report address the role of the CCT Program, implementation, funding and costs, accomplishments, project descriptions, legislative history, program history, environmental aspects, and project contacts. The project descriptions describe the technology and provides a brief summary of the demonstration results.

  12. Think City Electric Vehicle Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Ford Motor Company

    2005-03-01

    The THINK city Electric Vehicle (EV) Demonstration Program Project, initiated late 2001, has been successfully completed as of April 2005. US. Partners include Federal, State and Municipal agencies as well as commercial partners. Phase I, consisting of placement of the vehicles in demonstration programs, was completed in 2002. Phase II, the monitoring of these programs was completed in 2004. Phase III, the decommissioning and/or exporting of vehicles concluded in 2005. Phase I--the Program successfully assigned 192 EV's with customers (including Hertz) in the state of California, 109 in New York (including loaner and demo vehicles), 16 in Georgia, 7 to customers outside of the US and 52 in Ford's internal operations in Dearborn Michigan for a total of 376 vehicles. The Program was the largest operating Urban EV Demonstration Project in the United States. Phase II--the monitoring of the operational fleet was ongoing and completed in 2004, and all vehicles were returned throughout 2004 and 2005. The Department of Energy (DOE) was involved with the monitoring of the New York Power Authority/THINK Clean Commute Program units through partnership with Electric Transportation Engineering Corporation (ETEC), which filed separate reports to DOE. The remainder of the field fleet was monitored through Ford's internal operations. Vehicles were retired from lease operation throughout the program for various operator reasons. Some of the vehicles were involved in re-leasing operations. At the end of the program, 376 vehicles had been involved, 372 of which were available for customer use while 4 were engineering prototype and study vehicles. Phase III--decommissioning and/or export of vehicles. In accordance with the NHTSA requirement, City vehicles could not remain in the United States past their three-year allowed program timeframe. At the end of leases, City vehicles have been decommissioned and/or exported to KamKorp in Norway.

  13. Fuel Gas Demonstration Plant Program. Volume I. Demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    The objective of this project is for Babcock Contractors Inc. (BCI) to provide process designs, and gasifier retort design for a fuel gas demonstration plant for Erie Mining Company at Hoyt Lake, Minnesota. The fuel gas produced will be used to supplement natural gas and fuel oil for iron ore pellet induration. The fuel gas demonstration plant will consist of five stirred, two-stage fixed-bed gasifier retorts capable of handling caking and non-caking coals, and provisions for the installation of a sixth retort. The process and unit design has been based on operation with caking coals; however, the retorts have been designed for easy conversion to handle non-caking coals. The demonstration unit has been designed to provide for expansion to a commercial plant (described in Commercial Plant Package) in an economical manner.

  14. Clean Coal Technology Demonstration Program: Program update 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-03-01

    The Clean Coal Technology Demonstration Program (also referred to as the CCT Program) is a $6.9 billion cost-shared industry/government technology development effort. The program is to demonstrate a new generation of advanced coal-based technologies, with the most promising technologies being moved into the domestic and international marketplace. Technology has a vital role in ensuring that coal can continue to serve U.S. energy interests and enhance opportunities for economic growth and employment while meeting the national committment to a clean and healthy global environment. These technologies are being advanced through the CCT Program. The CCT Program supports three substantive national objectives: ensuring a sustainable environment through technology; enhancing energy efficiency and reliability; providing opportunities for economic growth and employment. The technologies being demonstrated under the CCT Program reduce the emissions of sulfur oxides, nitrogen oxides, greenhouse gases, hazardous air pollutants, solid and liquid wastes, and other emissions resulting from coal use or conversion to other fuel forms. These emissions reductions are achieved with efficiencies greater than or equal to currently available technologies.

  15. Clean Coal Technology Demonstration Program. Program update 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-04-01

    The Clean Coal Technology Demonstration Program (CCT Program) is a $7.14 billion cost-shared industry/government technology development effort. The program is to demonstrate a new generation of advanced coal-based technologies, with the most promising technologies being moved into the domestic and international marketplace. Clean coal technologies being demonstrated under the CCT program are creating the technology base that allows the nation to meet its energy and environmental goals efficiently and reliably. The fact that most of the demonstrations are being conducted at commercial scale, in actual user environments, and under conditions typical of commercial operations allows the potential of the technologies to be evaluated in their intended commercial applications. The technologies are categorized into four market sectors: advanced electric power generation systems; environmental control devices; coal processing equipment for clean fuels; and industrial technologies. Sections of this report describe the following: Role of the Program; Program implementation; Funding and costs; The road to commercial realization; Results from completed projects; Results and accomplishments from ongoing projects; and Project fact sheets. Projects include fluidized-bed combustion, integrated gasification combined-cycle power plants, advanced combustion and heat engines, nitrogen oxide control technologies, sulfur dioxide control technologies, combined SO{sub 2} and NO{sub x} technologies, coal preparation techniques, mild gasification, and indirect liquefaction. Industrial applications include injection systems for blast furnaces, coke oven gas cleaning systems, power generation from coal/ore reduction, a cyclone combustor with S, N, and ash control, cement kiln flue gas scrubber, and pulse combustion for steam coal gasification.

  16. Clean coal technology demonstration program: Program update 1996-97

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-10-01

    The Clean Coal Technology Demonstration Program (known as the CCT Program) reached a significant milestone in 1996 with the completion of 20 of the 39 active projects. The CCT Program is responding to a need to demonstrate and deploy a portfolio of technologies that will assure the U.S. recoverable coal reserves of 297 billion tons could continue to supply the nation`s energy needs economically and in a manner that meets the nation`s environmental objectives. This portfolio of technologies includes environmental control devices that contributed to meeting the accords on transboundary air pollution recommended by the Special Envoys on Acid Rain in 1986. Operational, technical, environmental, and economic performance information and data are now flowing from highly efficient, low-emission, advanced power generation technologies that will enable coal to retain its prominent role into the next millennium. Further, advanced technologies are emerging that will enhance the competitive use of coal in the industrial sector, such as in steelmaking. Coal processing technologies will enable the entire coal resource base to be used while complying with environmental requirements. These technologies are producing products used by utilities and industrial processes. The capability to coproduce products, such as liquid and solid fuels, electricity, and chemicals, is being demonstrated at a commercial scale by projects in the CCT Program. In summary, this portfolio of technologies is satisfying the national need to maintain a multifuel energy mix in which coal is a key component because of its low-cost, availability, and abundant supply within the nation`s borders.

  17. A simple demonstration of corrosion cells

    Science.gov (United States)

    Guichelaar, Philip J.; Williams, Molly W.

    1990-01-01

    The objective is to reinforce and enhance the understanding of galvanic cells, anode cathode reactions and polarization phenomena. Complete instructions are given for laboratory demonstration to be performed by students.

  18. Detonation duct gas generator demonstration program

    Science.gov (United States)

    Wortman, Andrew; Brinlee, Gayl A.; Othmer, Peter; Whelan, Michael A.

    1991-01-01

    The feasibility of the generation of detonation waves moving periodically across high speed channel flow is experimentally demonstrated. Such waves are essential to the concept of compressing requirements and increasing the engine pressure compressor with the objective of reducing conventional compressor requirements and increasing the engine thermodynamic efficiency through isochoric energy addition. By generating transient transverse waves, rather than standing waves, shock wave losses are reduced by an order of magnitude. The ultimate objective is to use such detonation ducts downstream of a low pressure gas turbine compressor to produce a high overall pressure ratio thermodynamic cycle. A 4 foot long, 1 inch x 12 inch cross-section, detonation duct was operated in a blow-down mode using compressed air reservoirs. Liquid or vapor propane was injected through injectors or solenoid valves located in the plenum or the duct itself. Detonation waves were generated when the mixture was ignited by a row of spark plugs in the duct wall. Problems with fuel injection and mixing limited the air speeds to about Mach 0.5, frequencies to below 10 Hz, and measured pressure ratios of about 5 to 6. The feasibility of the gas dynamic compression was demonstrated and the critical problem areas were identified.

  19. Tubular solid oxide fuel cell demonstration activities

    Energy Technology Data Exchange (ETDEWEB)

    Veyo, S.E.

    1995-08-01

    The development of a viable fuel cell driven electrical power generation system involves not only the development of cell and stack technology, but also the development of the overall system concept, the strategy for control, and the ancillary subsystems. The design requirements used to guide system development must reflect a customer focus in order to evolve a commercial product. In order to obtain useful customer feedback, Westinghouse has practiced the deployment with customers of fully integrated, automatically controlled, packaged solid oxide fuel cell power generation systems. These field units have served to demonstrate to customers first hand the beneficial attributes of the SOFC, to expose deficiencies through experience in order to guide continued development, and to garner real world feedback and data concerning not only cell and stack parameters, but also transportation, installation, permitting and licensing, start-up and shutdown, system alarming, fault detection, fault response, and operator interaction.

  20. Demonstration of Passive Fuel Cell Thermal Management Technology

    Science.gov (United States)

    Burke, Kenneth A.; Jakupca, Ian; Colozza, Anthony; Wynne, Robert; Miller, Michael; Meyer, Al; Smith, William

    2012-01-01

    The NASA Glenn Research Center is developing advanced passive thermal management technology to reduce the mass and improve the reliability of space fuel cell systems for the NASA Exploration program. The passive thermal management system relies on heat conduction within highly thermally conductive cooling plates to move the heat from the central portion of the cell stack out to the edges of the fuel cell stack. Using the passive approach eliminates the need for a coolant pump and other cooling loop components within the fuel cell system which reduces mass and improves overall system reliability. Previous development demonstrated the performance of suitable highly thermally conductive cooling plates and integrated heat exchanger technology to collect the heat from the cooling plates (Ref. 1). The next step in the development of this passive thermal approach was the demonstration of the control of the heat removal process and the demonstration of the passive thermal control technology in actual fuel cell stacks. Tests were run with a simulated fuel cell stack passive thermal management system outfitted with passive cooling plates, an integrated heat exchanger and two types of cooling flow control valves. The tests were run to demonstrate the controllability of the passive thermal control approach. Finally, successful demonstrations of passive thermal control technology were conducted with fuel cell stacks from two fuel cell stack vendors.

  1. CHP Fuel Cell Durability Demonstration - Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Petrecky, James; Ashley, Christopher J

    2014-07-21

    Plug Power has managed a demonstration project that has tested multiple units of its high-temperature, PEM fuel cell system in micro-combined heat and power (μ-CHP) applications in California. The specific objective of the demonstration project was to substantiate the durability of GenSys Blue, and, thereby, verify its technology and commercial readiness for the marketplace. In the demonstration project, Plug Power, in partnership with the National Fuel Cell Research Center (NFCRC) at the University of California, Irvine (UCI), and Sempra, will execute two major tasks: • Task 1: Internal durability/reliability fleet testing. Six GenSys Blue units will be built and will undergo an internal test regimen to estimate failure rates. This task was modified to include 3 GenSys Blue units installed in a lab at UCI. • Task 2: External customer testing. Combined heat and power units will be installed and tested in real-world residential and/or light commercial end user locations in California.

  2. National Fuel Cell Electric Vehicle Learning Demonstration Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Wipke, K.; Sprik, S.; Kurtz, J.; Ramsden, T.; Ainscough, C.; Saur, G.

    2012-07-01

    This report discusses key analysis results based on data from early 2005 through September 2011 from the U.S. Department of Energy's (DOE's) Controlled Hydrogen Fleet and Infrastructure Validation and Demonstration Project, also referred to as the National Fuel Cell Electric Vehicle (FCEV) Learning Demonstration. This report serves as one of many mechanisms to help transfer knowledge and lessons learned within various parts of DOE's Fuel Cell Technologies Program, as well as externally to other stakeholders. It is the fifth and final such report in a series, with previous reports being published in July 2007, November 2007, April 2008, and September 2010.

  3. Alternative-fueled truck demonstration natural gas program: Caterpillar G3406LE development and demonstration

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-06-01

    In 1990, the California Energy Commission, the South Coast Air Quality Management District, and the Southern California Gas Company joined together to sponsor the development and demonstration of compressed natural gas engines for Class 8 heavy-duty line-haul trucking applications. This program became part of an overall Alternative-Fueled Truck Demonstration Program, with the goal of advancing the technological development of alternative-fueled engines. The demonstration showed natural gas to be a technically viable fuel for Class 8 truck engines.

  4. Clean coal technologies: Research, development, and demonstration program plan

    Energy Technology Data Exchange (ETDEWEB)

    1993-12-01

    The US Department of Energy, Office of Fossil Energy, has structured an integrated program for research, development, and demonstration of clean coal technologies that will enable the nation to use its plentiful domestic coal resources while meeting environmental quality requirements. The program provides the basis for making coal a low-cost, environmentally sound energy choice for electric power generation and fuels production. These programs are briefly described.

  5. Programmed cell death

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    The purpose of this conference to provide a multidisciplinary forum for exchange of state-of-the-art information on the role programmed cell death plays in normal development and homeostasis of many organisms. This volume contains abstracts of papers in the following areas: invertebrate development; immunology/neurology; bcl-2 family; biochemistry; programmed cell death in viruses; oncogenesis; vertebrate development; and diseases.

  6. INTEGRATED GASIFICATION COMBINED CYCLE PROJECT 2 MW FUEL CELL DEMONSTRATION

    Energy Technology Data Exchange (ETDEWEB)

    FuelCell Energy

    2005-05-16

    With about 50% of power generation in the United States derived from coal and projections indicating that coal will continue to be the primary fuel for power generation in the next two decades, the Department of Energy (DOE) Clean Coal Technology Demonstration Program (CCTDP) has been conducted since 1985 to develop innovative, environmentally friendly processes for the world energy market place. The 2 MW Fuel Cell Demonstration was part of the Kentucky Pioneer Energy (KPE) Integrated Gasification Combined Cycle (IGCC) project selected by DOE under Round Five of the Clean Coal Technology Demonstration Program. The participant in the CCTDP V Project was Kentucky Pioneer Energy for the IGCC plant. FuelCell Energy, Inc. (FCE), under subcontract to KPE, was responsible for the design, construction and operation of the 2 MW fuel cell power plant. Duke Fluor Daniel provided engineering design and procurement support for the balance-of-plant skids. Colt Engineering Corporation provided engineering design, fabrication and procurement of the syngas processing skids. Jacobs Applied Technology provided the fabrication of the fuel cell module vessels. Wabash River Energy Ltd (WREL) provided the test site. The 2 MW fuel cell power plant utilizes FuelCell Energy's Direct Fuel Cell (DFC) technology, which is based on the internally reforming carbonate fuel cell. This plant is capable of operating on coal-derived syngas as well as natural gas. Prior testing (1992) of a subscale 20 kW carbonate fuel cell stack at the Louisiana Gasification Technology Inc. (LGTI) site using the Dow/Destec gasification plant indicated that operation on coal derived gas provided normal performance and stable operation. Duke Fluor Daniel and FuelCell Energy developed a commercial plant design for the 2 MW fuel cell. The plant was designed to be modular, factory assembled and truck shippable to the site. Five balance-of-plant skids incorporating fuel processing, anode gas oxidation, heat recovery

  7. Start-Up of the Colorectal Cancer Screening Demonstration Program

    Directory of Open Access Journals (Sweden)

    Amy DeGroff, MPH

    2008-04-01

    Full Text Available IntroductionIn 2005, the Centers for Disease Control and Prevention funded five sites to implement the Colorectal Cancer Screening Demonstration Program (CRCSDP. An evaluation is being conducted that includes a multiple case study. Case study results for the start-up period, the time between initial funding and screening initiation, provide details about the program models and start-up process and reveal important lessons learned.MethodsThe multiple case study includes all five CRCSDP sites, each representing a unique case. Data were collected from August 2005 through September 2006 from documents, observations, and more than 70 interviews with program staff and stakeholders.ResultsSites differed by geographic service area, screening modality selected, and service delivery structure. Program models were influenced by two factors: preexisting infrastructure and the need to adapt programs to fit local service delivery structures. Several sites modeled program components after their National Breast and Cervical Cancer Early Detection Program. Medical advisory boards convened by all sites provided clinical support for developing program policies and quality assurance plans. Partnerships with comprehensive cancer control programs facilitated access to financial and in-kind resources.ConclusionThe program models developed by the CRCSDP sites offer a range of prototypes. Case study results suggest benefits in employing a multidisciplinary staff team, assembling a medical advisory board, collaborating with local partners, using preexisting resources, designing programs that are easily incorporated into existing service delivery systems, and planning for adequate start-up time.

  8. Direct Methanol Fuel Cell Prototype Demonstration for Consumer Electronics Applications

    Energy Technology Data Exchange (ETDEWEB)

    Carlstrom, Charles, M., Jr.

    2009-07-07

    This report is the final technical report for DOE Program DE-FC36-04GO14301 titled “Direct Methanol Fuel Cell Prototype Demonstration for Consumer Electronics Applications”. Due to the public nature of this report some of the content reported in confidential reports and meetings to the DOE is not covered in detail in this report and some of the content has been normalized to not show actual values. There is a comparison of the projects accomplishments with the objectives, an overview of some of the key subsystem work, and a review of the three levels of prototypes demonstrated during the program. There is also a description of the eventual commercial product and market this work is leading towards. The work completed under this program has significantly increased the understanding of how Direct Methanol Fuel Cells (DMFC) can be deployed successfully to power consumer electronic devices. The prototype testing has demonstrated the benefits a direct methanol fuel cell system has over batteries typically used for powering consumer electronic devices. Three generations of prototypes have been developed and tested for performance, robustness and life. The technologies researched and utilized in the fuel cell stack and related subsystems for these prototypes are leveraged from advances in other industries such as the hydrogen fueled PEM fuel cell industry. The work under this program advanced the state of the art of direct methanol fuel cells. The system developed by MTI micro fuel cells aided by this program differs significantly from conventional DMFC designs and offers compelling advantages in the areas of performance, life, size, and simplicity. The program has progressed as planned resulting in the completion of the scope of work and available funding in December 2008. All 18 of the final P3 prototypes builds have been tested and the results showed significant improvements over P2 prototypes in build yield, initial performance, and durability. The systems have

  9. Hybrid Propulsion Demonstration Program 250K Hybrid Motor

    Science.gov (United States)

    Story, George; Zoladz, Tom; Arves, Joe; Kearney, Darren; Abel, Terry; Park, O.

    2003-01-01

    The Hybrid Propulsion Demonstration Program (HPDP) program was formed to mature hybrid propulsion technology to a readiness level sufficient to enable commercialization for various space launch applications. The goal of the HPDP was to develop and test a 250,000 pound vacuum thrust hybrid booster in order to demonstrate hybrid propulsion technology and enable manufacturing of large hybrid boosters for current and future space launch vehicles. The HPDP has successfully conducted four tests of the 250,000 pound thrust hybrid rocket motor at NASA's Stennis Space Center. This paper documents the test series.

  10. The role of a technology demonstration program for future reactors

    International Nuclear Information System (INIS)

    A comprehensive technology demonstration program is seen as an important component of the overall safety case, especially for a novel technology. The objective of such a program is defined as providing objective and auditable evidence that the technology will meet or exceed the relevant requirements. Various aspects of such a program are identified and then discussed in some details in this presentation. We will show how the need for such a program is anchored in fundamental safety principles. Attributes of the program, means of achieving its objective, roles of participants, as well as key steps are all elaborated. It will be argued that to prove a novel technology, the designer will have to combine several activities such as the use of operational experience, prototyping of the technology elements, conduct of experiments and tests under representative conditions, as well as modeling and analysis. Importance of availability of experimental facilities and qualified scientific and technical staff is emphasized. A solid technology demonstration program will facilitate and speed up regulatory evaluations of licensing applications. (author)

  11. Development of a Federally Funded Demonstration Colorectal Cancer Screening Program

    Directory of Open Access Journals (Sweden)

    Janet Royalty, MS

    2008-04-01

    Full Text Available Colorectal cancer is the second leading cause of cancer-related mortality among U.S. adults. In 2004, treatment costs for colorectal cancer were $8.4 billion.There is substantial evidence that colorectal cancer incidence and mortality are reduced with regular screening. The natural history of this disease is also well described: most colorectal cancers develop slowly from preexisting polyps. This slow development provides an opportunity to intervene with screening tests, which can either prevent colorectal cancer through the removal of polyps or detect it at an early stage. However, much less is known about how best to implement an effective colorectal cancer screening program. Screening rates are low, and uninsured persons, low-income persons, and persons who have not visited a physician within a year are least likely to be screened.Although the Centers for Disease Control and Prevention (CDC has 15 years of experience supporting the National Breast and Cervical Cancer Early Detection Program for the underserved population, a similar national program for colorectal cancer is not in place. To explore the feasibility of implementing a national program for the underserved U.S. population and to learn which settings and which program models are most viable and cost-effective, CDC began a 3-year colorectal cancer screening demonstration program in 2005.This article describes briefly this demonstration program and the process CDC used to design it and to select program sites. The multiple-methods evaluation now under way to assess the program’s feasibility and describe key outcomes is also detailed. Evaluation results will be used to inform future activities related to organized screening for colorectal cancer.

  12. Texas LPG fuel cell development and demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2004-07-26

    The State Energy Conservation Office has executed its first Fuel Cell Project which was awarded under a Department of Energy competitive grant process. The Texas LPG Fuel Processor Development and Fuel Cell Demonstration Program is a broad-based public/private partnership led by the Texas State Energy Conservation Office (SECO). Partners include the Alternative Fuels Research and Education Division (AFRED) of the Railroad Commission of Texas; Plug Power, Inc., Latham, NY, UOP/HyRadix, Des Plaines, IL; Southwest Research Institute (SwRI), San Antonio, TX; the Texas Natural Resource Conservation Commission (TNRCC), and the Texas Department of Transportation (TxDOT). The team proposes to mount a development and demonstration program to field-test and evaluate markets for HyRadix's LPG fuel processor system integrated into Plug Power's residential-scale GenSys(TM) 5C (5 kW) PEM fuel cell system in a variety of building types and conditions of service. The program's primary goal is to develop, test, and install a prototype propane-fueled residential fuel cell power system supplied by Plug Power and HyRadix in Texas. The propane industry is currently funding development of an optimized propane fuel processor by project partner UOP/HyRadix through its national checkoff program, the Propane Education and Research Council (PERC). Following integration and independent verification of performance by Southwest Research Institute, Plug Power and HyRadix will produce a production-ready prototype unit for use in a field demonstration. The demonstration unit produced during this task will be delivered and installed at the Texas Department of Transportation's TransGuide headquarters in San Antonio, Texas. Simultaneously, the team will undertake a market study aimed at identifying and quantifying early-entry customers, technical and regulatory requirements, and other challenges and opportunities that need to be addressed in planning commercialization of the units

  13. FUEL CELL BUS DEMONSTRATION IN MEXICO CITY

    Science.gov (United States)

    The report discusses the performance of a cull-size, zero-emission, Proton Exchange Membrane (PEM) fuel-cell-powered transit bus in the atmospheric environment of Mexico City. To address the air quality problems caused by vehicle emissions in Mexico City, a seminar on clean vehic...

  14. Early Site Permit Demonstration Program: Plant parameters envelope report

    International Nuclear Information System (INIS)

    The Early Site Permit (ESP) Demonstration Program is the nuclear industry's initiative for piloting the early resolution of siting-related issues before the detailed design proceedings of the combined operating license review. The ESP Demonstration Program consists of three phases. The plant parameters envelopes task is part of Phase 1, which addresses the generic review of applicable federal regulations and develops criteria for safety and environmental assessment of potential sites. The plant parameters envelopes identify parameters that characterize the interface between an ALWR design and a potential site, and quantify the interface through values selected from the Utility Requirements Documents, vendor design information, or engineering assessments. When augmented with site-specific information, the plant parameters envelopes provide sufficient information to allow ESPs to be granted based on individual ALWR design information or enveloping design information for the evolutionary, passive, or generic ALWR plants. This document is expected to become a living document when used by future applicants

  15. An inspection demonstration program/a quality system

    Energy Technology Data Exchange (ETDEWEB)

    Cox, L.D.

    1995-12-31

    Mobil relies on tubular manufacturers to consistently supply OCTG which complies with all material requirements of API Specification 5CT. This paper details the Mobil Inspection Demonstration Program; Mobil`s method for qualifying ultrasonic inspection systems for use during Mobil receiving inspections. Additionally, the basic fundamentals of the Mobil quality system and acceptance sampling are presented with an emphasis on tracking short-term and long-term supplier performance.

  16. NUCLEAR HYDROGEN DEVERLOPMENT AND DEMONSTRATION PROGRAM IN KOREA

    Institute of Scientific and Technical Information of China (English)

    林昌奎; 朴原奭; 张锺和

    2004-01-01

    The Korea Atomic Energy Research Institute (KAERI) launched a nuclear hydrogen program to develop and demonstrate a hydrogen mass production system by 2019. The key feature of the system is to produce the hydrogen from water using nuclear power. No fossil fuel (energy) will be used and no greenhouse gas will be emitted. The high temperature gas-cooled reactor (HTGR), which has high safety characteristics with competitive economy, is being developed to provide a high temperature heat (about 1000℃) requir...

  17. Pecan Street Grid Demonstration Program. Final technology performance report

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2015-02-10

    This document represents the final Regional Demonstration Project Technical Performance Report (TPR) for Pecan Street Inc.’s (Pecan Street) Smart Grid Demonstration Program, DE-OE-0000219. Pecan Street is a 501(c)(3) smart grid/clean energy research and development organization headquartered at The University of Texas at Austin (UT). Pecan Street worked in collaboration with Austin Energy, UT, Environmental Defense Fund (EDF), the City of Austin, the Austin Chamber of Commerce and selected consultants, contractors, and vendors to take a more detailed look at the energy load of residential and small commercial properties while the power industry is undergoing modernization. The Pecan Street Smart Grid Demonstration Program signed-up over 1,000 participants who are sharing their home or businesses’s electricity consumption data with the project via green button protocols, smart meters, and/or a home energy monitoring system (HEMS). Pecan Street completed the installation of HEMS in 750 homes and 25 commercial properties. The program provided incentives to increase the installed base of roof-top solar photovoltaic (PV) systems, plug-in electric vehicles with Level 2 charging, and smart appliances. Over 200 participants within a one square mile area took advantage of Austin Energy and Pecan Street’s joint PV incentive program and installed roof-top PV as part of this project. Of these homes, 69 purchased or leased an electric vehicle through Pecan Street’s PV rebate program and received a Level 2 charger from Pecan Street. Pecan Street studied the impacts of these technologies along with a variety of consumer behavior interventions, including pricing models, real-time feedback on energy use, incentive programs, and messaging, as well as the corresponding impacts on Austin Energy’s distribution assets.The primary demonstration site was the Mueller community in Austin, Texas. The Mueller development, located less than three miles from the Texas State Capitol

  18. Milliken Clean Coal Technology Demonstration Project. Project performance summary, Clean Coal Technology Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2002-11-30

    The New York State Electric & Gas Corporation (NYSEG) demonstrated a combination of technologies at its Milliken Station in Lansing, New York, designed to: (1) achieve high sulfur dioxide (SO2) capture efficiency, (2) bring nitrogen oxide (NOx) emissions into compliance with Clean Air Act Amendments of 1990 (CAAA), (3) maintain high station efficiency, and (4) eliminate waste water discharge. This project is part of the U.S. Department of Energy's (DOE) Clean Coal Technology Demonstration Program (CCTDP) established to address energy and environmental concerns related to coal use. DOE sought cost-shared partnerships with industry through five nationally competed solicitations to accelerate commercialization of the most promising advance coal-based power generation and pollution control technologies. The CCTDP, valued at over five billion dollars, has significantly leveraged federal funding by forging effective partnerships founded on sound principles. For every federal dollar invested, CCTDP participants have invested two dollars. These participants include utilities, technology developers, state governments, and research organizations. The project presented here was one of nine selected in January 1991 from 33 proposals submitted in response to the program's fourth solicitation.

  19. Photovoltaic test and demonstration project. [residential energy program

    Science.gov (United States)

    Forestieri, A. F.; Brandhorst, H. W., Jr.; Deyo, J. N.

    1976-01-01

    The considered project consists of three subprojects related to applications, device performance and diagnostics, and endurance testing. The objectives of the applications subproject include the determination of the operating characteristics for a variety of photovoltaic conversion systems. A system test facility is being constructed in this connection and a prototype residence experiment is to be conducted. Market demand for solar cells is to be stimulated by demonstrating suitability of solar cells for specific near-term applications. Activities conducted in connection with device performance studies and diagnostics are also discussed along with developments in the area of endurance testing.

  20. The current status of performance demonstration program in Taiwan

    International Nuclear Information System (INIS)

    The Institute of Nuclear Energy Research is holding an ultrasonic testing (UT) performance demonstration (PD) program for the nondestructive testing crew in nuclear power plants. This program is for qualifying personnel's manual detection skills on stainless steel piping and carbon steel piping welds. Artificial flaws embedded in welds comprise two types of circumferential and axial ones. Examinees are required to detect and size flaw lengths. Statistical analyses, including accuracy rates, detection rates and relative sizing errors, are made over examinees' detection data. Based upon eighteen examinees' output in six PD sessions, the overall accuracy rate is about 90%, with stainless steel and carbon steel piping yielding similar results. The length sizing errors, however, depend on piping materials and flaw orientations. The statistical results can provide both regulatory authorities and utilities with a conservative evaluation of the confidence level of realistic UT results in nuclear power plants. (authors)

  1. Northwest Hazardous Waste Research, Development, and Demonstration Center: Program Plan

    International Nuclear Information System (INIS)

    The Northwest Hazardous Waste Research, Development, and Demonstration Center was created as part of an ongoing federal effort to provide technologies and methods that protect human health and welfare and environment from hazardous wastes. The Center was established by the Superfund Amendments and Reauthorization Act (SARA) to develop and adapt innovative technologies and methods for assessing the impacts of and remediating inactive hazardous and radioactive mixed-waste sites. The Superfund legislation authorized $10 million for Pacific Northwest Laboratory to establish and operate the Center over a 5-year period. Under this legislation, Congress authorized $10 million each to support research, development, and demonstration (RD and D) on hazardous and radioactive mixed-waste problems in Idaho, Montana, Oregon, and Washington, including the Hanford Site. In 1987, the Center initiated its RD and D activities and prepared this Program Plan that presents the framework within which the Center will carry out its mission. Section 1.0 describes the Center, its mission, objectives, organization, and relationship to other programs. Section 2.0 describes the Center's RD and D strategy and contains the RD and D objectives, priorities, and process to be used to select specific projects. Section 3.0 contains the Center's FY 1988 operating plan and describes the specific RD and D projects to be carried out and their budgets and schedules. 9 refs., 18 figs., 5 tabs

  2. Performance Demonstration Program Plan for Analysis of Simulated Headspace Gases

    Energy Technology Data Exchange (ETDEWEB)

    Carlsbad Field Office

    2007-11-13

    The Performance Demonstration Program (PDP) for headspace gases distributes blind audit samples in a gas matrix for analysis of volatile organic compounds (VOCs). Participating measurement facilities (i.e., fixed laboratories, mobile analysis systems, and on-line analytical systems) are located across the United States. Each sample distribution is termed a PDP cycle. These evaluation cycles provide an objective measure of the reliability of measurements performed for transuranic (TRU) waste characterization. The primary documents governing the conduct of the PDP are the Quality Assurance Program Document (QAPD) (DOE/CBFO-94-1012) and the Waste Isolation Pilot Plant (WIPP) Waste Analysis Plan (WAP) contained in the Hazardous Waste Facility Permit (NM4890139088-TSDF) issued by the New Mexico Environment Department (NMED). The WAP requires participation in the PDP; the PDP must comply with the QAPD and the WAP. This plan implements the general requirements of the QAPD and the applicable requirements of the WAP for the Headspace Gas (HSG) PDP. Participating measurement facilities analyze blind audit samples of simulated TRU waste package headspace gases according to the criteria set by this PDP Plan. Blind audit samples (hereafter referred to as PDP samples) are used as an independent means to assess each measurement facility’s compliance with the WAP quality assurance objectives (QAOs). To the extent possible, the concentrations of VOC analytes in the PDP samples encompass the range of concentrations anticipated in actual TRU waste package headspace gas samples. Analyses of headspace gases are required by the WIPP to demonstrate compliance with regulatory requirements. These analyses must be performed by measurement facilities that have demonstrated acceptable performance in this PDP. These analyses are referred to as WIPP analyses and the TRU waste package headspace gas samples on which they are performed are referred to as WIPP samples in this document

  3. Performance Demonstration Program Plan for Analysis of Simulated Headspace Gases

    Energy Technology Data Exchange (ETDEWEB)

    Carlsbad Field Office

    2007-11-19

    The Performance Demonstration Program (PDP) for headspace gases distributes blind audit samples in a gas matrix for analysis of volatile organic compounds (VOCs). Participating measurement facilities (i.e., fixed laboratories, mobile analysis systems, and on-line analytical systems) are located across the United States. Each sample distribution is termed a PDP cycle. These evaluation cycles provide an objective measure of the reliability of measurements performed for transuranic (TRU) waste characterization. The primary documents governing the conduct of the PDP are the Quality Assurance Program Document (QAPD) (DOE/CBFO-94-1012) and the Waste Isolation Pilot Plant (WIPP) Waste Analysis Plan (WAP) contained in the Hazardous Waste Facility Permit (NM4890139088-TSDF) issued by the New Mexico Environment Department (NMED). The WAP requires participation in the PDP; the PDP must comply with the QAPD and the WAP. This plan implements the general requirements of the QAPD and the applicable requirements of the WAP for the Headspace Gas (HSG) PDP. Participating measurement facilities analyze blind audit samples of simulated TRU waste package headspace gases according to the criteria set by this PDP Plan. Blind audit samples (hereafter referred to as PDP samples) are used as an independent means to assess each measurement facility’s compliance with the WAP quality assurance objectives (QAOs). To the extent possible, the concentrations of VOC analytes in the PDP samples encompass the range of concentrations anticipated in actual TRU waste package headspace gas samples. Analyses of headspace gases are required by the WIPP to demonstrate compliance with regulatory requirements. These analyses must be performed by measurement facilities that have demonstrated acceptable performance in this PDP. These analyses are referred to as WIPP analyses and the TRU waste package headspace gas samples on which they are performed are referred to as WIPP samples in this document

  4. Performance Demonstration Program Plan for Analysis of Simulated Headspace Gases

    International Nuclear Information System (INIS)

    The Performance Demonstration Program (PDP) for headspace gases distributes sample gases of volatile organic compounds (VOCs) for analysis. Participating measurement facilities (i.e., fixed laboratories, mobile analysis systems, and on-line analytical systems) are located across the United States. Each sample distribution is termed a PDP cycle. These evaluation cycles provide an objective measure of the reliability of measurements performed for transuranic (TRU) waste characterization. The primary documents governing the conduct of the PDP are the Quality Assurance Program Document (QAPD) (DOE/CBFO-94-1012) and the Waste Isolation Pilot Plant (WIPP) Waste Analysis Plan (WAP) contained in the Hazardous Waste Facility Permit (NM4890139088-TSDF) issued by the New Mexico Environment Department (NMED). The WAP requires participation in the PDP; the PDP must comply with the QAPD and the WAP. This plan implements the general requirements of the QAPD and the applicable requirements of the WAP for the Headspace Gas (HSG) PDP. Participating measurement facilities analyze blind audit samples of simulated TRU waste package headspace gases according to the criteria set by this PDP Plan. Blind audit samples (hereafter referred to as PDP samples) are used as an independent means to assess each measurement facility's compliance with the WAP quality assurance objectives (QAOs). To the extent possible, the concentrations of VOC analytes in the PDP samples encompass the range of concentrations anticipated in actual TRU waste package headspace gas samples. Analyses of headspace gases are required by the WIPP to demonstrate compliance with regulatory requirements. These analyses must be performed by measurement facilities that have demonstrated acceptable performance in this PDP. These analyses are referred to as WIPP analyses and the TRU waste package headspace gas samples on which they are performed are referred to as WIPP samples in this document. Participating measurement

  5. Performance in the WIPP nondestructive assay performance demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    Marcinkiewicz, C.J. [Consolidated Technical Services, Inc., Frederick, MD (United States); Connolly, M.J.; Becker, G.K. [Lockheed Martin Idaho Technologies Company, Idaho Falls, ID (United States)

    1997-11-01

    Measurement facilities performing nondestructive assay (NDA) of wastes intended for disposal at the United States Department of Energy (DOE) Waste Isolation Pilot Plant (WIPP) are required to demonstrate their ability to meet specific Quality Assurance Objectives (QAOs). This demonstration is performed, in part, by participation in the NDA Performance Demonstration Program (PDP). The PDP is funded and managed by the Carlsbad Area Office (CAO) of DOE and is conducted by the Idaho National Engineering Laboratory. It tests the characteristics of precision, system bias and/or total uncertainty through the measurement of variable, blind combinations of simulated waste drums and certified radioactive standards. Each facility must successfully participate in the PDP using each different type of measurement system planned for use in waste characterization. The first cycle of the PDP using each different type of measurement system planned for use in waste characterization. The first cycle of the PDP was completed in July 1996 and the second is scheduled for completion by December 1996. Seven sites reported data in cycle 1 for 11 different measurement systems. This paper describes the design and operation of the PDP and provides the performance data from cycle 1. It also describes the preliminary results from cycle 2 and updates the status and future plans for the NDA PDP. 4 refs., 9 figs., 11 tabs.

  6. Lunar and Planetary Science XXXV: Education Programs Demonstrations

    Science.gov (United States)

    2004-01-01

    Reports from the session on Education Programs Demonstration include:Hands-On Activities for Exploring the Solar System in K-14; Formal Education and Informal Settings;Making Earth and Space Science and Exploration Accessible; New Thematic Solar System Exploration Products for Scientists and Educators Engaging Students of All Ages with Research-related Activities: Using the Levers of Museum Reach and Media Attention to Current Events; Astronomy Village: Use of Planetary Images in Educational Multimedia; ACUMEN: Astronomy Classes Unleashed: Meaningful Experiences for Neophytes; Unusual Guidebook to Terrestrial Field Work Studies: Microenvironmental Studies by Landers on Planetary Surfaces (New Atlas in the Series of the Solar System Notebooks on E tv s University, Hungary); and The NASA ADS: Searching, Linking and More.

  7. Thin-Layer Fuel Cell for Teaching and Classroom Demonstrations

    Science.gov (United States)

    Shirkhanzadeh, M.

    2009-01-01

    A thin-layer fuel cell is described that is simple and easy to set up and is particularly useful for teaching and classroom demonstrations. The cell is both an electrolyzer and a fuel cell and operates using a thin layer of electrolyte with a thickness of approximately 127 micrometers and a volume of approximately 40 microliters. As an…

  8. Jersey City energy conservation demonstration program. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Newbold, R.F.

    1978-08-01

    The Aerospace Corporation, the City Government, and the Board of Education of Jersey City have conducted a group of energy-conservation experiments to explore a number of conservation techniques believed to offer quick payback and to be of wide applicability. Experiments include the updating and/or rehabilitation of the energy-consuming features of old buildings and installation of devices designed to minimize energy losses caused by human error or laxity. Specific examples include: upgrading of the deteriorated and inefficient steam-distribution system of the city hall (originally constructed in 1894); an extensive program of reducing infiltration in an old school building; use of several timing devices in connection with heating, ventilation, and lighting systems to encourage energy-conservation practices; retrofit of school classrooms with high-pressure sodium lamps; and demonstration of practical and cost-effective ways of increasing the efficiency of conventional steam boilers. The report presents: the nature of the selected experiments; technical, human, and organizational factors that proved significant in performing and evaluating the experiments; discussions of observations and lessons learned; and general recommendations for an extended program of energy conservation in local governments. It is emphasized that, in retrofit of existing buildings, the unexpected is commonplace; and the habits and attitudes of building occupants are elements of the system that must always be taken into account. This report shows the benefits of energy saving, cost saving, and added comfort that may be attained by retrofitting old buildings, noting typical complications that arise. The effectiveness of the conservation methods is presented in terms of costs relative to effective payback periods calculated from results of their application in Jersey City.

  9. Clean Coal Technology Demonstration Program: Program update 1991 (as of December 31, 1991)

    International Nuclear Information System (INIS)

    The Clean Coal Technology Demonstration Program (also referred to as the CCT Program) is a government and industry cofunded technology development effort to demonstrate a new generation of innovative coal utilization processes in a series of large-scale ''showcase'' facilities built across the country. The program takes the most promising advanced coal-based technologies and moves them into the commercial marketplace through demonstration. These demonstrations are on a scale large enough to generate all the data, from design, construction and operation, that are necessary for the private sector to judge commercial potential and make informed, confident decisions on commercial readiness. The CCT Program has been identified in the National Energy Strategy as major initiative supporting the strategy's overall goals to: increase efficiency of energy use; secure future energy supplies; enhance environmental quality; fortify foundations. The technologies being demonstrated under the CCT Program when commercially available will enable coal to reach its full potential as a source of energy for the nation and the international marketplace. The goal of the program is to furnish the US and international energy marketplaces with a number of advanced, highly efficient, and environmentally acceptable coal-using technologies

  10. Montana Integrated Carbon to Liquids (ICTL) Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Fiato, Rocco; Sharma, Ramesh; Allen, Mark; Peyton, Brent; Macur, Richard; Cameron, Jemima

    2013-09-30

    Integrated carbon-to-liquids technology (ICTL) incorporates three basic processes for the conversion of a wide range of feedstocks to distillate liquid fuels: (1) Direct Microcatalytic Coal Liquefaction (MCL) is coupled with biomass liquefaction via (2) Catalytic Hydrodeoxygenation and Isomerization (CHI) of fatty acid methyl esters (FAME) or trigylceride fatty acids (TGFA) to produce liquid fuels, with process derived (3) CO{sub 2} Capture and Utilization (CCU) via algae production and use in BioFertilizer for added terrestrial sequestration of CO{sub 2}, or as a feedstock for MCL and/or CHI. This novel approach enables synthetic fuels production while simultaneously meeting EISA 2007 Section 526 targets, minimizing land use and water consumption, and providing cost competitive fuels at current day petroleum prices. ICTL was demonstrated with Montana Crow sub-bituminous coal in MCL pilot scale operations at the Energy and Environmental Research Center at the University of North Dakota (EERC), with related pilot scale CHI studies conducted at the University of Pittsburgh Applied Research Center (PARC). Coal-Biomass to Liquid (CBTL) Fuel samples were evaluated at the US Air Force Research Labs (AFRL) in Dayton and greenhouse tests of algae based BioFertilizer conducted at Montana State University (MSU). Econometric modeling studies were also conducted on the use of algae based BioFertilizer in a wheat-camelina crop rotation cycle. We find that the combined operation is not only able to help boost crop yields, but also to provide added crop yields and associated profits from TGFA (from crop production) for use an ICTL plant feedstock. This program demonstrated the overall viability of ICTL in pilot scale operations. Related work on the Life Cycle Assessment (LCA) of a Montana project indicated that CCU could be employed very effectively to reduce the overall carbon footprint of the MCL/CHI process. Plans are currently being made to conduct larger-scale process

  11. Montana Integrated Carbon to Liquids (ICTL) Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Fiato, Rocco; Sharma, Ramesh; Allen, Mark; Peyton, Brent; Macur, Richard; Cameron, Jemima

    2013-09-30

    Integrated carbon‐to‐liquids technology (ICTL) incorporates three basic processes for the conversion of a wide range of feedstocks to distillate liquid fuels: (1) Direct Microcatalytic Coal Liquefaction (MCL) is coupled with biomass liquefaction via (2) Catalytic Hydrodeoxygenation and Isomerization (CHI) of fatty acid methyl esters (FAME) or trigylceride fatty acids (TGFA) to produce liquid fuels, with process derived (3) CO{sub 2} Capture and Utilization (CCU) via algae production and use in BioFertilizer for added terrestrial sequestration of CO{sub 2}, or as a feedstock for MCL and/or CHI. This novel approach enables synthetic fuels production while simultaneously meeting EISA 2007 Section 526 targets, minimizing land use and water consumption, and providing cost competitive fuels at current day petroleum prices. ICTL was demonstrated with Montana Crow sub‐bituminous coal in MCL pilot scale operations at the Energy and Environmental Research Center at the University of North Dakota (EERC), with related pilot scale CHI studies conducted at the University of Pittsburgh Applied Research Center (PARC). Coal‐Biomass to Liquid (CBTL) Fuel samples were evaluated at the US Air Force Research Labs (AFRL) in Dayton and greenhouse tests of algae based BioFertilizer conducted at Montana State University (MSU). Econometric modeling studies were also conducted on the use of algae based BioFertilizer in a wheat‐camelina crop rotation cycle. We find that the combined operation is not only able to help boost crop yields, but also to provide added crop yields and associated profits from TGFA (from crop production) for use an ICTL plant feedstock. This program demonstrated the overall viability of ICTL in pilot scale operations. Related work on the Life Cycle Assessment (LCA) of a Montana project indicated that CCU could be employed very effectively to reduce the overall carbon footprint of the MCL/CHI process. Plans are currently being made to conduct larger

  12. Osmotic Pressure, Bacterial Cell Walls, and Penicillin: A Demonstration.

    Science.gov (United States)

    Lennox, John E.

    1984-01-01

    An easily constructed apparatus that models the effect of penicillin on the structure of bacterial cells is described. Background information and procedures for using the apparatus during a classroom demonstration are included. (JN)

  13. Cooperative Demonstration Program To Train Aviation Maintenance Technicians. Final Report.

    Science.gov (United States)

    Alabama Aviation and Technical Coll., Ozark.

    The Alabama Aviation and Technical College, working with representatives of the aviation industry, the military, the Alabama Department of Aeronautics, and the Federal Aviation Administration, developed a training program for aviation maintenance technicians. The program also aimed to emphasize and expand opportunities for minorities, females, and…

  14. Geothermal energy, research, development and demonstration program. Third annual report

    Energy Technology Data Exchange (ETDEWEB)

    1979-03-01

    The following topics are covered: the geothermal resource potential in the U.S., national geothermal utilization estimates, the Federal geothermal development strategy and program, Federal progress and achievements FY 1978, regional progress FY 1978, and Federal program plans for FY 1979. (MHR)

  15. FY-94 buried waste integrated demonstration program report

    Energy Technology Data Exchange (ETDEWEB)

    1994-11-01

    The Buried Waste Integrated Demonstration (BWID) supports the applied research, development, demonstration, and evaluation of a multitude of advanced technologies. These technologies are being integrated to form a comprehensive remediation system for the effective and efficient remediation of buried waste. These efforts are identified and coordinated in support of the U.S. Department of Energy (DOE), Environmental Restoration and Waste Management (ER/WM) needs and objectives. This document summarizes previous demonstrations and describes the FY-94 BWID technology development and demonstration activities. Sponsored by the DOE Office of Technology Development (OTD), BWID works with universities and private industry to develop these technologies, which are being transferred to the private sector for use nationally and internationally. A public participation policy has been established to provide stakeholders with timely and accurate information and meaningful opportunities for involvement in the technology development and demonstration process.

  16. FY-94 buried waste integrated demonstration program report

    International Nuclear Information System (INIS)

    The Buried Waste Integrated Demonstration (BWID) supports the applied research, development, demonstration, and evaluation of a multitude of advanced technologies. These technologies are being integrated to form a comprehensive remediation system for the effective and efficient remediation of buried waste. These efforts are identified and coordinated in support of the U.S. Department of Energy (DOE), Environmental Restoration and Waste Management (ER/WM) needs and objectives. This document summarizes previous demonstrations and describes the FY-94 BWID technology development and demonstration activities. Sponsored by the DOE Office of Technology Development (OTD), BWID works with universities and private industry to develop these technologies, which are being transferred to the private sector for use nationally and internationally. A public participation policy has been established to provide stakeholders with timely and accurate information and meaningful opportunities for involvement in the technology development and demonstration process

  17. Santa Clara County Planar Solid Oxide Fuel Cell Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Fred Mitlitsky; Sara Mulhauser; David Chien; Deepak Shukla; David Weingaertner

    2009-11-14

    The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project demonstrated the technical viability of pre-commercial PSOFC technology at the County 911 Communications headquarters, as well as the input fuel flexibility of the PSOFC. PSOFC operation was demonstrated on natural gas and denatured ethanol. The Santa Clara County Planar Solid Oxide Fuel Cell (PSOFC) project goals were to acquire, site, and demonstrate the technical viability of a pre-commercial PSOFC technology at the County 911 Communications headquarters. Additional goals included educating local permit approval authorities, and other governmental entities about PSOFC technology, existing fuel cell standards and specific code requirements. The project demonstrated the Bloom Energy (BE) PSOFC technology in grid parallel mode, delivering a minimum 15 kW over 8760 operational hours. The PSOFC system demonstrated greater than 81% electricity availability and 41% electrical efficiency (LHV net AC), providing reliable, stable power to a critical, sensitive 911 communications system that serves geographical boundaries of the entire Santa Clara County. The project also demonstrated input fuel flexibility. BE developed and demonstrated the capability to run its prototype PSOFC system on ethanol. BE designed the hardware necessary to deliver ethanol into its existing PSOFC system. Operational parameters were determined for running the system on ethanol, natural gas (NG), and a combination of both. Required modeling was performed to determine viable operational regimes and regimes where coking could occur.

  18. Low-E Retrofit Demonstration and Educational Program

    Energy Technology Data Exchange (ETDEWEB)

    Culp, Thomas D [Birch Point Consulting LLC; Wiehagen, Joseph [Home Innovation Research Labs, Inc.; Drumheller, S Craig [Home Innovation Research Labs, Inc.; Siegel, John [Quanta Technologies Inc.; Stratmoen, Todd [Larson Manufacturing

    2013-11-16

    The objective of this project was to demonstrate the capability of low-emissivity (low-E) storm windows / panels and low-E retrofit glazing systems to significantly and cost effectively improve the energy efficiency of both existing residential and commercial buildings. The key outcomes are listed below: RESIDENTIAL CASE STUDIES: (a) A residential case study in two large multifamily apartment buildings in Philadelphia showed a substantial 18-22% reduction in heating energy use and a 9% reduction in cooling energy use by replacing old clear glass storm windows with modern low-E storm windows. Furthermore, the new low-E storm windows reduced the overall apartment air leakage by an average of 10%. (b) Air leakage testing on interior low-E panels installed in a New York City multifamily building over windows with and without AC units showed that the effective leakage area of the windows was reduced by 77-95%. (c) To study the use of low-E storm windows in a warmer mixed climate with a balance of both heating and cooling, 10 older homes near Atlanta with single pane windows were tested with three types of exterior storm windows: clear glass, low-E glass with high solar heat gain, and low-E glass with lower solar heat gain. The storm windows significantly reduced the overall home air leakage by an average of 17%, or 3.7 ACH50. Considerably high variability in the data made it difficult to draw strong conclusions about the overall energy usage, but for heating periods, the low-E storm windows showed approximately 15% heating energy savings, whereas clear storm windows were neutral in performance. For cooling periods, the low-E storm windows showed a wide range of performance from 2% to over 30% cooling energy savings. Overall, the study showed the potential for significantly more energy savings from using low-E glass versus no storm window or clear glass storm windows in warmer mixed climates, but it is difficult to conclusively say whether one type of low-E performed

  19. Industrial Fuel Gas Demonstration Plant Program. Volume 1. Demonstration plant environmental analysis (Deliverable No. 27)

    Energy Technology Data Exchange (ETDEWEB)

    Gray, Robert W.; Swift, Richard J.; Krause, Arthur J.; Berkey, Edgar

    1979-08-01

    This environmental report describes the proposed action to construct, test and operate a coal gasification demonstration plant in Memphis, Tennessee, under the co-sponsorship of the Memphis Light, Gas and Water Division (MLGW) and the US Department of Energy (DOE). This document is Volume I of a three-volume Environmental Report. Volume I consists of the Summary, Introduction and the Description of the Proposed Action. Volume II consists of the Description of the Existing Environment. Volume III contains the Environmental Impacts of the Proposed Action, Mitigating Measures and Alternatives to the Proposed Action.

  20. Industrial Fuel Gas Demonstration Plant Program. Volume III. Demonstration plant environmental analysis (Deliverable No. 27)

    Energy Technology Data Exchange (ETDEWEB)

    1979-08-01

    An Environmental Report on the Memphis Light, Gas and Water Division Industrial Fuel Demonstration Plant was prepared for submission to the US Department of Energy under Contract ET-77-C-01-2582. This document is Volume III of a three-volume Environmental Report. Volume I consists of the Summary, Introduction and the Description of the Proposed Action. Volume II consists of the Description of the Existing Environment. Volume III contains the Environmental Impacts of the Proposed Action, Mitigating Measures and Alternatives to the Proposed Action.

  1. National Fuel Cell Electric Vehicle Learning Demonstration Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Wipke, K. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sprik, S. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Kurtz, J. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ramsden, T. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ainscough, C. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Saur, G. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2012-07-01

    This report discusses key analysis results based on data from early 2005 through September 2011 from the U.S. Department of Energy’s (DOE’s) Controlled Hydrogen Fleet and Infrastructure Validation and Demonstration Project, also referred to as the National Fuel Cell Electric Vehicle (FCEV) Learning Demonstration. It is the fifth and final such report in a series, with previous reports being published in July 2007, November 2007, April 2008, and September 2010.

  2. Hybrid fuel cell bus demonstration: advanced technology moves bus forward

    International Nuclear Information System (INIS)

    The Province of Manitoba has been actively pursuing hydrogen since 2001 as one part of a portfolio of renewable energy alternatives. Six priority hydrogen actions have been underway covering a variety of opportunities, including two recently completed major transit bus and refueling demonstrations. A brief overview of Manitoba's activities on hydrogen will be provided, emphasizing the lessons learned from recent projects such as the hydrogen Hybrid Fuel Cell Bus demonstration, and in particular implications for the research community. (author)

  3. Efficient Swath Mapping Laser Altimetry Demonstration Instrument Incubator Program

    Science.gov (United States)

    Yu, Anthony W.; Krainak, Michael A,; Harding, David J.; Abshire, James B.; Sun, Xiaoli; Cavanaugh, John; Valett, Susan

    2010-01-01

    In this paper we will discuss our eighteen-month progress of a three-year Instrument Incubator Program (IIP) funded by NASA Earth Science Technology Office (ESTO) on swath mapping laser altimetry system. This paper will discuss the system approach, enabling technologies and instrument concept for the swath mapping laser altimetry.

  4. 78 FR 39759 - Rental Assistance Demonstration: Final Program Notice

    Science.gov (United States)

    2013-07-02

    ... voucher program; or if a qualifying mortgage pre-payment would trigger the provision of enhanced vouchers... that HUD published on March 8, 2012, at 77 FR 14029, RAD has two separate components: First Component... may apply to HUD to convert to one of two forms of Section 8 Housing Assistance Payment...

  5. Demonstration of an E-mailed Worksite Nutrition Intervention Program

    Directory of Open Access Journals (Sweden)

    Gladys Block

    2004-10-01

    Full Text Available Introduction Dietary fat and low fruit and vegetable intake are linked to many chronic diseases, and U.S. population intake does not meet recommendations. Interventions are needed that incorporate effective behavior-change principles and that can be delivered inexpensively to large segments of the population. Methods Employees at a corporate worksite were invited to participate in a program, delivered entirely by e-mail, to reduce dietary fat and increase fruit and vegetable intake. Behavior-change principles underlying the intervention included tailoring to the participant’s dietary lifestyle, baseline assessment and feedback about dietary intake, family participation, and goal setting. Assessment, tailoring, and delivery was fully automated. The program was delivered weekly to participants’ e-mail inboxes for 12 weeks. Each e-mail included information on nutrition or on the relationship between diet and health, dietary tips tailored to the individual, and small goals to try for the next week. In this nonrandomized pilot study, we assessed technical feasibility, acceptability to employees, improvement in Stage of Change, increase in fruit and vegetable consumption, and decrease in fat intake. Results Approximately one third (n = 84 of employees who were offered the 12-week program signed up for it, and satisfaction was high. There was significant improvement in Stage of Change: 74% of those not already at the top had forward movement (P < .001. In addition, results suggest significant increase in fruit and vegetable consumption (0.73 times/day, P < .001 and significant decrease in intake of fat sources (-0.39 times/day, P < .001. Conclusion This inexpensive program is feasible and appears to be effective. A randomized controlled trial is needed.

  6. DEMONSTRATION OF FUEL CELLS TO RECOVER ENERGY FROM LANDFILL GAS - PHASE III. DEMONSTRATION TESTS - PHASE IV. GUIDELINES AND RECOMMENDATIONS- VOLUME 1. TECHNICAL REPORT

    Science.gov (United States)

    The report summarizes the results of a four-phase program to demonstrate that fuel cell energy recovery using a commercial phosphoric acid fuel cell is both environmentally sound and commercially feasible. Phase I, a conceptual design and evaluation study, addressed the technical...

  7. Alternate retrieval technology demonstrations program - test report (ARD Environmental, Inc.)

    International Nuclear Information System (INIS)

    A prototype vehicle, control system, and waste and water scavenging system were designed and fabricated with essentially the full capabilities of the vehicle system proposed by ARD Environmental. A test tank mockup, including riser and decontamination chamber were designed and fabricated, and approximately 830 cubic feet of six varieties of waste simulants poured. The tests were performed by ARD Environmental personnel at its site in Laurel, Maryland, from 4/22/97 through 5/2/97. The capabilities tested were deployment and retrieval, extended mobility and productivity, the ability to operate the system using video viewing only, retrieval after simulated failure, and retrieval and decontamination. Testing commenced with deployment of the vehicle into the tank. Deployment was accomplished using a crane and auxiliary winch to position the vehicle and lower it through the decontamination chamber, into the 36'' diameter x 6' high riser, and touch down on the waste field in the tank. The initial mobility tests were conducted immediately after deployment, prior to sluicing, as the waste field exhibited the greatest amount of variation at this time. This test demonstrated the ability of the vehicle to maneuver over the simulated waste field, and the ability of the operator to work with only video viewing available. In addition, the ability of the vehicle to right itself after being turned on its side was demonstrated. The production rate was evaluated daily through the testing period by measuring the surface and estimating the amount of material removed. The test demonstrated the ability of the vehicle to reduce the waste surface using 400 psi (nominal) water jets, scavenge water and material from the work area, and move to any location, even in the relatively confined space of the 20' diameter test tank. In addition, the ability to sluice to a remote scavenging module was demonstrated. The failure mode test demonstrated the ability to retrieve a stuck vehicle by pulling

  8. 75 FR 82567 - Federal Acquisition Regulation; Repeal of the Small Business Competitiveness Demonstration Program

    Science.gov (United States)

    2010-12-30

    ... of the Small Business Competitiveness Demonstration Program, to meet the requirements of section 1335... (15 U.S.C. 644 note), the Small Business Competitiveness Demonstration Program. In accordance with the Act, the repeal of the Small Business Competitiveness Demonstration Program became...

  9. Caterpillar MorElectric DOE Idle Reduction Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    John Bernardi

    2007-09-30

    This project titled 'Demonstration of the New MorElectric{trademark} Technology as an Idle Reduction Solution' is one of four demonstration projects awarded by the US Department of Energy in 2002. The goal of these demonstration and evaluation projects was to gather objective in-use information on the performance of available idle reduction technologies by characterizing the cost; fuel, maintenance, and engine life savings; payback; and user impressions of various systems and techniques. In brief, the Caterpillar Inc. project involved applying electrically driven accessories for cab comfort during engine-off stops and for reducing fuel consumption during on-highway operation. Caterpillar had equipped and operated five new trucks with the technology in conjunction with International Truck and Engine Corporation and COX Transfer. The most significant result of the project was a demonstrated average idle reduction of 13.8% for the 5 truck MEI fleet over the control fleet. It should be noted that the control fleet trucks were also equipped with an idle reduction device that would start and stop the main engine automatically in order to maintain cab temperature. The control fleet idle usage would have been reduced by 3858 hours over the 2 year period with the MEI system installed, or approximately 2315 gallons of fuel less (calculations assume a fuel consumption of 0.6 gallons per hour for the 13 liter engine at idle). The fuel saved will be significantly larger for higher displacement engines without idle reduction equipment such as the engine auto start/stop device used by COX Transfer. It is common for engines to consume 1.0 gallons per hour which would increase the fuel savings to approximately 1260 gallons per truck per year of typical idling (1800 hours idle/yr).

  10. Intergovernmental Advanced Stationary PEM Fuel Cell System Demonstration Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Rich Chartrand

    2011-08-31

    A program to complete the design, construction and demonstration of a PEMFC system fuelled by Ethanol, LPG or NG for telecom applications was initiated in October 2007. Early in the program the economics for Ethanol were shown to be unfeasible and permission was given by DOE to focus on LPG only. The design and construction of a prototype unit was completed in Jun 2009 using commercially available PEM FC stack from Ballard Power Systems. During the course of testing, the high pressure drop of the stack was shown to be problematic in terms of control and stability of the reformer. Also, due to the power requirements for air compression the overall efficiency of the system was shown to be lower than a similar system using internally developed low pressure drop FC stack. In Q3 2009, the decision was made to change to the Plug power stack and a second prototype was built and tested. Overall net efficiency was shown to be 31.5% at 3 kW output. Total output of the system is 6 kW. Using the new stack hardware, material cost reduction of 63% was achieved over the previous Alpha design. During a November 2009 review meeting Plug Power proposed and was granted permission, to demonstrate the new, commercial version of Plug Power's telecom system at CERL. As this product was also being tested as part of a DOE Topic 7A program, this part of the program was transferred to the Topic 7A program. In Q32008, the scope of work of this program was expanded to include a National Grid demonstration project of a micro-CHP system using hightemperature PEM technology. The Gensys Blue system was cleared for unattended operation, grid connection, and power generation in Aug 2009 at Union College in NY state. The system continues to operate providing power and heat to Beuth House. The system is being continually evaluated and improvements to hardware and controls will be implemented as more is learned about the system's operation. The program is instrumental in improving the

  11. Ansaldo programs on fuel cell vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Marcenaro, B.G.; Federici, F. [Ansaldo Ricerche Srl, Genova (Italy)

    1996-12-31

    The growth in traffic and the importance of maintaining a stable ecology at the global scale, particularly with regard to atmospheric pollution, raises the necessity to realize a new generation of vehicles which are more efficient, more economical and compatible with the environment. At European level, the Car of Tomorrow task force has identified fuel cells as a promising alternative propulsion system. Ansaldo Ricerche has been involved in the development of fuel cell vehicles since the early nineties. Current ongoing programs relates to: (1) Fuel cell bus demonstrator (EQHEPP BUS) Test in 1996 (2) Fuel cell boat demonstrator (EQHHPP BOAT) Test in 1997 (3) Fuel cell passenger car prototype (FEVER) Test in 1997 (4) 2nd generation Fuel cell bus (FCBUS) 1996-1999 (5) 2nd generation Fuel cell passenger car (HYDRO-GEN) 1996-1999.

  12. Municipal solid waste combustor ash demonstration program `the boathouse`

    Energy Technology Data Exchange (ETDEWEB)

    Roethel, F.J.; Breslin, V.T.

    1995-08-01

    The report presents the results of a research program designed to examine the engineering and environmental acceptability of using municipal solid waste (MSW) combustor ash as an aggregate substitute in the manufacture of construction quality cement blocks. 350 tons of MSW combustor ash was combined with Portland and Cement to form standard hollow masonary blocks. These stabilized combustor ash (SCA) blocks were used to construct a boathouse on the campus of the University at Stony Brook. Air samples collected within the boathouse were examined and compared to ambient air samples for the presence and concentrations of suspended particulate, and vapor phase PCDD/PCDF, volatile and semi-volatile organic compounds and volatile mercury. Rainwater samples following contact with the boathouse walls were collected and analyzed for the presence of trace elements. Soil samples were collected prior to and following the construction of the boathouse.

  13. FAST-Net optical interconnection prototype demonstration program

    Science.gov (United States)

    Haney, Michael W.; Christensen, Marc P.; Milojkovik, P.; Ekman, Jeremy T.; Chandramani, Premanand; Rozier, Richard G.; Kiamilev, Fouad E.; Liu, Yue; Hibbs-Brenner, Mary K.; Nohava, Jim; Kalweit, Edith; Bounnak, Sommy; Marta, Terry; Walterson, B.

    1998-05-01

    This paper reports progress toward the experimental demonstration of a smart pixel based optical interconnection prototype currently being developed under the Free-space Accelerator for Switching Terabit Networks (FAST-Net) project. The prototype system incorporates 2D arrays of monolithically integrated high- bandwidth vertical cavity surface emitting lasers (VCSELs) and photodetectors (PDs). A key aspect of the FAST-Net concept is that all smart pixels are distributed across a single multi-chip plane. This plane is connected to itself via an optical system that consists of an array of matched lenses (one for each smart pixel chip position) and a mirror. The optical interconnect system implements a global point-to-point shuffle pattern. The interleaved 2D arrays of VCSELs and PDs in the prototype are arranged on a clustered self-similar grid pattern with a closest element pitch of 100 micrometers . The circular VCSEL elements have a diameter of 10 micrometers and the square PDs have an active region that is 50 micrometers wide. These arrays are packaged and mounted on circuit boards along with the CMOS driver, receiver, and FPGA controller chips. Micro-positioning mounts are used to effect alignment that is consistent with current MCM chip placement accuracy. Shuffled optical data links between the multiple ICs have been demonstrated in preliminary evaluation of this system. These results suggest that a multi-Terabit optically interconnected MCM module is feasible.

  14. DOE standard compliance demonstration program: An office building example

    Energy Technology Data Exchange (ETDEWEB)

    Bailey, S.A.; Keller, J.M.; Wrench, L.E.; Williams, C.J.

    1993-06-01

    The US Department of Energy (DOE) issued interim new building energy standards (10 CFR 435 1989) to achieve maximum energy efficiency in the designs of new buildings. DOE then entered into a project to demonstrate and assess the impact of these standards on the design community. One area of focus was a test to see how a less conventional design-focused building would meet the standards` requirements -- DOE wanted to demonstrate that compliance with energy standards does not mean compromising the architectural intent of a building. This study, which was initiated at Pacific Northwest Laboratory (PNL), illustrated the process by which compliance with the standards can be proven for a highly {open_quotes}design-oriented{close_quotes} office building. The study also assessed the impact of the whole building simulation compliance alternatives on design. This report documents the compliance requirements, gives a description of the sample building chosen for the study, provides general guidance for the compliance process, documents the method of compliance that was undertaken for the sample building, presents the results of the study, and provides a recommendation on how the compliance requirements could be improved to reflect more realistic use types.

  15. Demonstration of a PC 25 Fuel Cell in Russia

    Energy Technology Data Exchange (ETDEWEB)

    John C. Trocciola; Thomas N. Pompa; Linda S. Boyd

    2004-09-01

    This project involved the installation of a 200kW PC25C{trademark} phosphoric-acid fuel cell power plant at Orgenergogaz, a Gazprom industrial site in Russia. In April 1997, a PC25C{trademark} was sold by ONSI Corporation to Orgenergogaz, a subsidiary of the Russian company ''Gazprom''. Due to instabilities in the Russian financial markets, at that time, the unit was never installed and started by Orgenergogaz. In October of 2001 International Fuel Cells (IFC), now known as UTC Fuel Cells (UTCFC), received a financial assistance award from the United States Department of Energy (DOE) entitled ''Demonstration of PC 25 Fuel Cell in Russia''. Three major tasks were part of this award: the inspection of the proposed site and system, start-up assistance, and installation and operation of the powerplant.

  16. Automotive Fuel Processor Development and Demonstration with Fuel Cell Systems

    Energy Technology Data Exchange (ETDEWEB)

    Nuvera Fuel Cells

    2005-04-15

    The potential for fuel cell systems to improve energy efficiency and reduce emissions over conventional power systems has generated significant interest in fuel cell technologies. While fuel cells are being investigated for use in many applications such as stationary power generation and small portable devices, transportation applications present some unique challenges for fuel cell technology. Due to their lower operating temperature and non-brittle materials, most transportation work is focusing on fuel cells using proton exchange membrane (PEM) technology. Since PEM fuel cells are fueled by hydrogen, major obstacles to their widespread use are the lack of an available hydrogen fueling infrastructure and hydrogen's relatively low energy storage density, which leads to a much lower driving range than conventional vehicles. One potential solution to the hydrogen infrastructure and storage density issues is to convert a conventional fuel such as gasoline into hydrogen onboard the vehicle using a fuel processor. Figure 2 shows that gasoline stores roughly 7 times more energy per volume than pressurized hydrogen gas at 700 bar and 4 times more than liquid hydrogen. If integrated properly, the fuel processor/fuel cell system would also be more efficient than traditional engines and would give a fuel economy benefit while hydrogen storage and distribution issues are being investigated. Widespread implementation of fuel processor/fuel cell systems requires improvements in several aspects of the technology, including size, startup time, transient response time, and cost. In addition, the ability to operate on a number of hydrocarbon fuels that are available through the existing infrastructure is a key enabler for commercializing these systems. In this program, Nuvera Fuel Cells collaborated with the Department of Energy (DOE) to develop efficient, low-emission, multi-fuel processors for transportation applications. Nuvera's focus was on (1) developing fuel

  17. 75 FR 14582 - Office of Special Education and Rehabilitative Services-Special Demonstration Programs-Model...

    Science.gov (United States)

    2010-03-26

    ... Office of Special Education and Rehabilitative Services--Special Demonstration Programs--Model Demonstration Projects To Improve Outcomes for Individuals Receiving Social Security Disability Insurance (SSDI... identify, develop, and implement model demonstration projects to improve outcomes for individuals...

  18. 75 FR 47798 - Office of Special Education and Rehabilitative Services-Special Demonstration Programs-Model...

    Science.gov (United States)

    2010-08-09

    ... Office of Special Education and Rehabilitative Services--Special Demonstration Programs--Model Demonstration Project To Improve Outcomes for Individuals Receiving Social Security Disability Insurance (SSDI..., develop, and implement a model demonstration project to improve outcomes for individuals receiving...

  19. Photovoltaic Test and Demonstration Project. [for solar cell power systems

    Science.gov (United States)

    Forestieri, A. F.; Brandhorst, H. W., Jr.; Deyo, J. N.

    1976-01-01

    The Photovoltaic Test and Demonstration Project was initiated by NASA in June, 1975, to develop economically feasible photovoltaic power systems suitable for a variety of terrestrial applications. Objectives include the determination of operating characteristic and lifetimes of a variety of solar cell systems and components and development of methodology and techniques for accurate measurements of solar cell and array performance and diagnostic measurements for solar power systems. Initial work will be concerned with residential applications, with testing of the first prototype system scheduled for June, 1976. An outdoor 10 kW array for testing solar power systems is under construction.

  20. Performance Demonstration Program Plan for Nondestructive Assay of Drummed Wastes for the TRU Waste Characterization Program

    International Nuclear Information System (INIS)

    Each testing and analytical facility performing waste characterization activities for the Waste Isolation Pilot Plant (WIPP) participates in the Performance Demonstration Program (PDP) to comply with the Transuranic Waste Acceptance Criteria for the Waste Isolation Pilot Plant (WAC) (DOE/WIPP-02-3122) and the Quality Assurance Program Document (QAPD) (CBFO-94-1012). The PDP serves as a quality control check for data generated in the characterization of waste destined for WIPP. Single blind audit samples are prepared and distributed to each of the facilities participating in the PDP. The PDP evaluates analyses of simulated headspace gases, constituents of the Resource Conservation and Recovery Act (RCRA), and transuranic (TRU) radionuclides using nondestructive assay (NDA) techniques.

  1. Industrial Fuel Gas Demonstration Plant Program: environmental permit compliance plan

    Energy Technology Data Exchange (ETDEWEB)

    Bodamer, Jr., James W.; Bocchino, Robert M.

    1979-11-01

    This Environmental Permit Compliance Plan is intended to assist the Memphis Light, Gas and Water Division in acquiring the necessary environmental permits for their proposed Industrial Fuel Gas Demonstration Plant in a time frame consistent with the construction schedule. Permits included are those required for installation and/or operation of gaseous, liquid and solid waste sources and disposal areas. Only those permits presently established by final regulations are described. The compliance plan describes procedures for obtaining each permit from identified federal, state and local agencies. The information needed for the permit application is presented, and the stepwise procedure to follow when filing the permit application is described. Information given in this plan was obtained by reviewing applicable laws and regulations and from telephone conversations with agency personnel on the federal, state and local levels. This Plan also presents a recommended schedule for beginning the work necessary to obtain the required environmental permits in order to begin dredging operations in October, 1980 and construction of the plant in September, 1981. Activity for several key permits should begin as soon as possible.

  2. Early Site Permit Demonstration Program: Regulatory criteria evaluation report

    International Nuclear Information System (INIS)

    The primary objective of the ESPDP is to demonstrate successfully the use of 10CFR52 to obtain ESPs for one or more US sites for one (or more) ALWR nuclear power plants. It is anticipated that preparation of the ESP application and interaction with NRC during the application review process will result not only in an ESP for the applicant(s) but also in the development of criteria and definition of processes, setting the precedent that facilitates ESPs for subsequent ESP applications. Because siting regulatory processes and acceptance criteria are contained in over 100 separate documents, comprehensive licensing and technical reviews were performed to establish whether the requirements and documentation are self-consistent, whether the acceptance criteria are sufficiently well-defined and clear, and whether the licensing process leading to the issuance of an ESP is unambiguously specified. The results of the technical and licensing evaluations are presented in this report. The purpose, background, and organization of the ESPDP is delineated in Section 1. Section 11 contains flowcharts defining siting application requirements, environmental report requirements, and emergency planning/preparedness requirements for ALWRS. The licensing and technical review results are presented in Section III

  3. Current status of demonstration for stationary fuel cell in Korea

    Energy Technology Data Exchange (ETDEWEB)

    Park, D.R. [Korea Gas Co., Ansan (Korea, Republic of). Research and Development Div.

    2009-07-01

    In 2006, the Korean Government launched the Residential Fuel Cell Monitoring Project to address the barriers to the widespread commercialization of fuel cells for residential power generation (RPG). This presentation reviewed the status on the development and dissemination of the residential fuel cell in Korea, with particular reference to a domestically developed proton exchange membrane (PEM) fuel cell for RPG. The purpose of the project was to find a commercial product that is suitable to Korean life style and to solve reliability issues regarding grid connection. The paper showed that although residential fuel cell systems are available in Korea, their capital cost remains high and system durability is not sufficient. This large-scale demonstration test project for residential fuel cell systems was launched in an effort to reduce the cost to levels that are acceptable for wide scale use. A total of 210 unit were tested in order to evaluate the technical level and to investigate the problems that must be solved for mass market introduction.

  4. Early Site Permit Demonstration Program: Recommendations for communication activities and public participation in the Early Site Permit Demonstration Program

    International Nuclear Information System (INIS)

    On October 24, 1992, President Bush signed into law the National Energy Policy Act of 1992. The bill is a sweeping, comprehensive overhaul of the Nation's energy laws, the first in more than a decade. Among other provisions, the National Energy Policy Act reforms the licensing process for new nuclear power plants by adopting a new approach developed by the US Nuclear Regulatory Commission (NRC) in 1989, and upheld in court in 1992. The NRC 10 CFR Part 52 rule is a three-step process that guarantees public participation at each step. The steps are: early site permit approval; standard design certifications; and, combined construction/operating licenses for nuclear power reactors. Licensing reform increases an organization's ability to respond to future baseload electricity generation needs with less financial risk for ratepayers and the organization. Costly delays can be avoided because design, safety and siting issues will be resolved before a company starts to build a plant. Specifically, early site permit approval allows for site suitability and acceptability issues to be addressed prior to an organization's commitment to build a plant. Responsibility for site-specific activities, including communications and public participation, rests with those organizations selected to try out early site approval. This plan has been prepared to assist those companies (referred to as sponsoring organizations) in planning their communications and public involvement programs. It provides research findings, information and recommendations to be used by organizations as a resource and starting point in developing their own plans

  5. Early Site Permit Demonstration Program: Recommendations for communication activities and public participation in the Early Site Permit Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-01-27

    On October 24, 1992, President Bush signed into law the National Energy Policy Act of 1992. The bill is a sweeping, comprehensive overhaul of the Nation`s energy laws, the first in more than a decade. Among other provisions, the National Energy Policy Act reforms the licensing process for new nuclear power plants by adopting a new approach developed by the US Nuclear Regulatory Commission (NRC) in 1989, and upheld in court in 1992. The NRC 10 CFR Part 52 rule is a three-step process that guarantees public participation at each step. The steps are: early site permit approval; standard design certifications; and, combined construction/operating licenses for nuclear power reactors. Licensing reform increases an organization`s ability to respond to future baseload electricity generation needs with less financial risk for ratepayers and the organization. Costly delays can be avoided because design, safety and siting issues will be resolved before a company starts to build a plant. Specifically, early site permit approval allows for site suitability and acceptability issues to be addressed prior to an organization`s commitment to build a plant. Responsibility for site-specific activities, including communications and public participation, rests with those organizations selected to try out early site approval. This plan has been prepared to assist those companies (referred to as sponsoring organizations) in planning their communications and public involvement programs. It provides research findings, information and recommendations to be used by organizations as a resource and starting point in developing their own plans.

  6. Low-E Retrofit Demonstration and Educational Program

    Energy Technology Data Exchange (ETDEWEB)

    Culp, Thomas D [Birch Point Consulting LLC; Wiehagen, Joseph [Home Innovation Research Labs, Inc.; Drumheller, S Craig [Home Innovation Research Labs, Inc.; Siegel, John [Quanta Technologies Inc.; Stratmoen, Todd [Larson Manufacturing

    2013-11-16

    The objective of this project was to demonstrate the capability of low-emissivity (low-E) storm windows / panels and low-E retrofit glazing systems to significantly and cost effectively improve the energy efficiency of both existing residential and commercial buildings. The key outcomes are listed below: RESIDENTIAL CASE STUDIES: (a) A residential case study in two large multifamily apartment buildings in Philadelphia showed a substantial 18-22% reduction in heating energy use and a 9% reduction in cooling energy use by replacing old clear glass storm windows with modern low-E storm windows. Furthermore, the new low-E storm windows reduced the overall apartment air leakage by an average of 10%. (b) Air leakage testing on interior low-E panels installed in a New York City multifamily building over windows with and without AC units showed that the effective leakage area of the windows was reduced by 77-95%. (c) To study the use of low-E storm windows in a warmer mixed climate with a balance of both heating and cooling, 10 older homes near Atlanta with single pane windows were tested with three types of exterior storm windows: clear glass, low-E glass with high solar heat gain, and low-E glass with lower solar heat gain. The storm windows significantly reduced the overall home air leakage by an average of 17%, or 3.7 ACH50. Considerably high variability in the data made it difficult to draw strong conclusions about the overall energy usage, but for heating periods, the low-E storm windows showed approximately 15% heating energy savings, whereas clear storm windows were neutral in performance. For cooling periods, the low-E storm windows showed a wide range of performance from 2% to over 30% cooling energy savings. Overall, the study showed the potential for significantly more energy savings from using low-E glass versus no storm window or clear glass storm windows in warmer mixed climates, but it is difficult to conclusively say whether one type of low-E performed

  7. Diesel fueled ship propulsion fuel cell demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    Kumm, W.H. [Arctic Energies Ltd., Severna Park, MD (United States)

    1996-12-31

    The paper describes the work underway to adapt a former US Navy diesel electric drive ship as a 2.4 Megawatt fuel cell powered, US Coast Guard operated, demonstrator. The Project will design the new configuration, and then remove the four 600 kW diesel electric generators and auxiliaries. It will design, build and install fourteen or more nominal 180 kW diesel fueled molten carbonate internal reforming direct fuel cells (DFCs). The USCG cutter VINDICATOR has been chosen. The adaptation will be carried out at the USCG shipyard at Curtis Bay, MD. A multi-agency (state and federal) cooperative project is now underway. The USCG prime contractor, AEL, is performing the work under a Phase III Small Business Innovation Research (SBIR) award. This follows their successful completion of Phases I and II under contract to the US Naval Sea Systems (NAVSEA) from 1989 through 1993 which successfully demonstrated the feasibility of diesel fueled DFCs. The demonstrated marine propulsion of a USCG cutter will lead to commercial, naval ship and submarine applications as well as on-land applications such as diesel fueled locomotives.

  8. Aqueous semi-solid flow cell: demonstration and analysis

    Energy Technology Data Exchange (ETDEWEB)

    Li, Z; Smith, KC; Dong, YJ; Baram, N; Fan, FY; Xie, J; Limthongkul, P; Carter, WC; Chiang, YM

    2013-01-01

    An aqueous Li-ion flow cell using suspension-based flow electrodes based on the LiTi2(PO4)(3)-LiFePO4 couple is demonstrated. Unlike conventional flow batteries, the semi-solid approach utilizes fluid electrodes that are electronically conductive. A model of simultaneous advection and electrochemical transport is developed and used to separate flow-induced losses from those due to underlying side reactions. The importance of plug flow to achieving high energy efficiency in flow batteries utilizing highly non-Newtonian flow electrodes is emphasized.

  9. City of Chula Vista hydrogen fuel cell bus demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    Gustafson, B.; Bamberger, B.

    1996-10-01

    Hydrogen as an energy carrier and fuel has potential for various uses including electricity, commercial, residential, transportation, and industrial. It is an energy carrier that can be produced from a variety of primary sources and potentially can accomplish these various uses while significantly reducing pollution by substituting for or reducing the use of fossil fuels. One of the most immediate and potentially viable roles for hydrogen as an energy carrier will be its use as a transportation fuel, especially in densely populated urban areas where automotive emissions contribute significantly to air pollution. The Department of Energy`s commitment to research and development of hydrogen as an alternative fuel, and California`s Zero Emission Vehicle (ZEV) requirements, both provide the impetus and favorable circumstance for demonstrating hydrogen as a transportation fuel on an urban bus system. The purpose of this project is to demonstrate the feasibility of using solid polymer fuel cells in a hydrogen-powered electric drive system for an urban transit bus application. Fuel cell buses use hydrogen fuel and oxygen from the air to produce electrical power with the only byproduct being pure water. Proton Exchange Membrane (PEM) fuel cells are proposed for this project. Current evidence suggests that fuel cells, which rely on hydrogen and a process known as proton exchange to generate their power, appear to have an infinite life span. All exhaust pollution is completely eliminated, resulting in a Zero Emission Vehicle (ZEV). An urban bus system offers the potential for developing a market for the production of hydrogen propulsion technology due to extensive vehicular use in densely populated areas experiencing pollution from numerous sources, and because the central garaging facilities or the bus system facilitates fueling and maintenance functions.

  10. 75 FR 43178 - Medicare Program; Solicitation for Proposals for the Medicare Imaging Demonstration

    Science.gov (United States)

    2010-07-23

    ... HUMAN SERVICES Centers for Medicare & Medicaid Services Medicare Program; Solicitation for Proposals for the Medicare Imaging Demonstration AGENCY: Centers for Medicare & Medicaid Services (CMS), HHS. ACTION... an opportunity to apply to participate in the Medicare Imaging Demonstration (MID) that...

  11. Performance Demonstration Program Plan for Nondestructive Assay of Drummed Wastes for the TRU Waste Characterization Program

    International Nuclear Information System (INIS)

    The Performance Demonstration Program (PDP) for Nondestructive Assay (NDA) is a test program designed to yield data on measurement system capability to characterize drummed transuranic (TRU) waste generated throughout the Department of Energy (DOE) complex. The tests are conducted periodically and provide a mechanism for the independent and objective assessment of NDA system performance and capability relative to the radiological characterization objectives and criteria of the Office of Characterization and Transportation (OCT). The primary documents requiring an NDA PDP are the Waste Acceptance Criteria for the Waste Isolation Pilot Plant (WAC), which requires annual characterization facility participation in the PDP, and the Quality Assurance Program Document (QAPD). This NDA PDP implements the general requirements of the QAPD and applicable requirements of the WAC. Measurement facilities must demonstrate acceptable radiological characterization performance through measurement of test samples comprised of pre-specified PDP matrix drum/radioactive source configurations. Measurement facilities are required to analyze the NDA PDP drum samples using the same procedures approved and implemented for routine operational waste characterization activities. The test samples provide an independent means to assess NDA measurement system performance and compliance per criteria delineated in the NDA PDP Plan. General inter-comparison of NDA measurement system performance among DOE measurement facilities and commercial NDA services can also be evaluated using measurement results on similar NDA PDP test samples. A PDP test sample consists of a 55-gallon matrix drum containing a waste matrix type representative of a particular category of the DOE waste inventory and nuclear material standards of known radionuclide and isotopic composition typical of DOE radioactive material. The PDP sample components are made available to participating measurement facilities as designated by the

  12. Programming Surface Chemistry with Engineered Cells.

    Science.gov (United States)

    Zhang, Ruihua; Heyde, Keith C; Scott, Felicia Y; Paek, Sung-Ho; Ruder, Warren C

    2016-09-16

    We have developed synthetic gene networks that enable engineered cells to selectively program surface chemistry. E. coli were engineered to upregulate biotin synthase, and therefore biotin synthesis, upon biochemical induction. Additionally, two different functionalized surfaces were developed that utilized binding between biotin and streptavidin to regulate enzyme assembly on programmable surfaces. When combined, the interactions between engineered cells and surfaces demonstrated that synthetic biology can be used to engineer cells that selectively control and modify molecular assembly by exploiting surface chemistry. Our system is highly modular and has the potential to influence fields ranging from tissue engineering to drug development and delivery.

  13. A Case Study of Technical Assistance to Demonstration Programs for Young Handicapped Children. Part III.

    Science.gov (United States)

    Behr, Shirley K.; And Others

    The report describes the third and final year of a 3-year case study of the technical assistance process as implemented by the Technical Assistance Development System (TADS) for the staffs of two demonstration programs for preschool handicapped children and their families. Following a review of TADS and the two demonstration programs, the…

  14. 20 CFR 416.250 - Experimental, pilot, and demonstration projects in the SSI program.

    Science.gov (United States)

    2010-04-01

    ... projects in the SSI program. 416.250 Section 416.250 Employees' Benefits SOCIAL SECURITY ADMINISTRATION... Because of Essential Persons § 416.250 Experimental, pilot, and demonstration projects in the SSI program... administration of the SSI program. These projects will test the advantages of altering certain...

  15. Safety analysis report for packaging (onsite) transuranic performance demonstration program sample packaging

    International Nuclear Information System (INIS)

    The Transuranic Performance Demonstration Program (TPDP) sample packaging is used to transport highway route controlled quantities of weapons grade (WG) plutonium samples from the Plutonium Finishing Plant (PFP) to the Waste Receiving and Processing (WRAP) facility and back. The purpose of these shipments is to test the nondestructive assay equipment in the WRAP facility as part of the Nondestructive Waste Assay PDP. The PDP is part of the U. S. Department of Energy (DOE) National TRU Program managed by the U. S. Department of Energy, Carlsbad Area Office, Carlsbad, New Mexico. Details of this program are found in CAO-94-1045, Performance Demonstration Program Plan for Nondestructive Assay for the TRU Waste Characterization Program (CAO 1994); INEL-96/0129, Design of Benign Matrix Drums for the Non-Destructive Assay Performance Demonstration Program for the National TRU Program (INEL 1996a); and INEL-96/0245, Design of Phase 1 Radioactive Working Reference Materials for the Nondestructive Assay Performance Demonstration Program for the National TRU Program (INEL 1996b). Other program documentation is maintained by the national TRU program and each DOE site participating in the program. This safety analysis report for packaging (SARP) provides the analyses and evaluations necessary to demonstrate that the TRU PDP sample packaging meets the onsite transportation safety requirements of WHC-CM-2-14, Hazardous Material Packaging and Shipping, for an onsite Transportation Hazard Indicator (THI) 2 packaging. This SARP, however, does not include evaluation of any operations within the PFP or WRAP facilities, including handling, maintenance, storage, or operating requirements, except as they apply directly to transportation between the gate of PFP and the gate of the WRAP facility. All other activities are subject to the requirements of the facility safety analysis reports (FSAR) of the PFP or WRAP facility and requirements of the PDP

  16. Secondary Schools Demonstration Project: Program Effects of School-Based Interventions on Antisocial Behaviour

    Science.gov (United States)

    Wright, Robin; Offord, David; John, Lindsay; Duku, Eric; DeWit, David

    2005-01-01

    This article describes the methodology and program effects of the Secondary Schools Demonstration Project (SSDP) conducted in four Ontario schools. The objective of the study was to evaluate the extent to which a universal program model of three interventions--cooperative learning; classroom management; and peer-helping approaches that included…

  17. Light microscopical demonstration and zonal distribution of parasinusoidal cells (Ito cells) in normal human liver

    DEFF Research Database (Denmark)

    Horn, T; Junge, Jette; Nielsen, O;

    1988-01-01

    The parasinusoidal cells of the liver (Ito cells) were demonstrated light microscopically in autopsy specimens fixed in formalin and stained with Oil red O after dichromate treatment. The method allows examination of large samples containing numerous acini. Quantitative assessment showed a zonal...

  18. Fuel Cell Vehicle Learning Demonstration: Spring 2008 Results; Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Wipke, K.; Sprik, S.; Kurtz, J.; Garbak, J.

    2008-04-01

    Conference paper presented at the 2008 National Hydrogen Association Meeting that describes the spring, 2008 results of the Controlled Hydrogen Fleet and Infrastructure Demonstration and Validation Project.

  19. DESIGN OF SMALL AUTOMATION WORK CELL SYSTEM DEMONSTRATIONS

    Energy Technology Data Exchange (ETDEWEB)

    C. TURNER; J. PEHL; ET AL

    2000-12-01

    The introduction of automation systems into many of the facilities dealing with the production, use and disposition of nuclear materials has been an ongoing objective. Many previous attempts have been made, using a variety of monolithic and, in some cases, modular technologies. Many of these attempts were less than successful, owing to the difficulty of the problem, the lack of maturity of the technology, and over optimism about the capabilities of a particular system. Consequently, it is not surprising that suggestions that automation can reduce worker Occupational Radiation Exposure (ORE) levels are often met with skepticism and caution. The development of effective demonstrations of these technologies is of vital importance if automation is to become an acceptable option for nuclear material processing environments. The University of Texas Robotics Research Group (UTRRG) has been pursuing the development of technologies to support modular small automation systems (each of less than 5 degrees-of-freedom) and the design of those systems for more than two decades. Properly designed and implemented, these technologies have a potential to reduce the worker ORE associated with work in nuclear materials processing facilities. Successful development of systems for these applications requires the development of technologies that meet the requirements of the applications. These application requirements form a general set of rules that applicable technologies and approaches need to adhere to, but in and of themselves are generally insufficient for the design of a specific automation system. For the design of an appropriate system, the associated task specifications and relationships need to be defined. These task specifications also provide a means by which appropriate technology demonstrations can be defined. Based on the requirements and specifications of the operations of the Advanced Recovery and Integrated Extraction System (ARIES) pilot line at Los Alamos National

  20. Waste immobilization demonstration program for the Hanford Site's Mixed Waste Facility

    International Nuclear Information System (INIS)

    This paper presents an overview of the Waste Receiving and Processing facility, Module 2A> waste immobilization demonstration program, focusing on the cooperation between Hanford Site, commercial, and international participants. Important highlights of the development and demonstration activities is discussed from the standpoint of findings that have had significant from the standpoint of findings that have had significant impact on the evolution of the facility design. A brief description of the future direction of the program is presented, with emphasis on the key aspects of the technologies that call for further detailed investigation

  1. 2015 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    Popovich, Neil

    2015-12-01

    In the past year, the DOE Hydrogen Program (the Program) made substantial progress toward its goals and objectives. The Program has conducted comprehensive and focused efforts to enable the widespread commercialization of hydrogen and fuel cell technologies in diverse sectors of the economy. With emphasis on applications that will effectively strengthen our nation's energy security and improve our stewardship of the environment, the Program engages in research, development, and demonstration of critical improvements in the technologies. Highlights of the Program's accomplishments can be found in the sub-program chapters of this report.

  2. 2012 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    2012-12-01

    In the past year, the DOE Hydrogen Program (the Program) made substantial progress toward its goals and objectives. The Program has conducted comprehensive and focused efforts to enable the widespread commercialization of hydrogen and fuel cell technologies in diverse sectors of the economy. With emphasis on applications that will effectively strengthen our nation's energy security and improve our stewardship of the environment, the Program engages in research, development, and demonstration of critical improvements in the technologies. Highlights of the Program's accomplishments can be found in the sub-program chapters of this report.

  3. Office of Technology Development Program for Research, Development, Demonstration, Testing, and Evaluation. FY 1993 Program Summary

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-01

    DOE has set a goal to clean up its complex and to bring all sites into compliance with applicable environmental regulations. This initiative is slated for completion by the year 2019. Four years ago there was no coordinated plan for identifying or cleaning these contaminated sites. Since 1989, DOE`s Office of Environmental Restoration and Waste Management has invested time, money, and manpower to establish a wide range of programs to meet this immense challenge. DOE is responsible for waste management and clean up of more than 100 contaminated installations in 36 states and territories. This includes 3,700 sites: over 26,000 acres, with hazardous or radioactive contaminated surface or groundwater, soil, or structures; over 26,000 acres requiring remediation, with the number growing as new sites are defined; 500 surplus facilities awaiting decontamination and decommissioning and approximately 5,000 peripheral properties (residences, businesses) that have soil contaminated with uranium tailings.

  4. Human pancreatic islet progenitor cells demonstrate phenotypic plasticity in vitro

    Indian Academy of Sciences (India)

    Maithili P Dalvi; Malati R Umrani; Mugdha V Joglekar; Anandwardhan A Hardikar

    2009-10-01

    Phenotypic plasticity is a phenomenon that describes the occurrence of 2 or more distinct phenotypes under diverse conditions. This article discusses the work carried out over the past few years in understanding the potential of human pancreatic islet-derived progenitors for cell replacement therapy in diabetes. The phenotypic plasticity exhibited by pancreatic progenitors during reversible epithelial-to-mesenchymal transition (EMT) and possible role of microRNAs in regulation of this process is also presented herein.

  5. Regulatory requirements of the integrated technology demonstration program, Savannah River Site (U)

    International Nuclear Information System (INIS)

    The integrated demonstration program at the Savannah River Site (SRS) involves demonstration, testing and evaluation of new characterization, monitoring, drilling and remediation technologies for soils and groundwater impacted by organic solvent contamination. The regulatory success of the demonstration program has developed as a result of open communications between the regulators and the technical teams involved. This open dialogue is an attempt to allow timely completion of applied environmental restoration demonstrations while meeting all applicable regulatory requirements. Simultaneous processing of multiple regulatory documents (satisfying RCRA, CERCLA, NEPA and various state regulations) has streamlined the overall permitting process. Public involvement is achieved as various regulatory documents are advertised for public comment consistent with the site's community relations plan. The SRS integrated demonstration has been permitted and endorsed by regulatory agencies, including the Environmental Protection Agency (EPA) and the South Carolina Department of Health and Environmental Control. EPA headquarters and regional offices are involved in DOE's integrated Demonstration Program. This relationship allows for rapid regulatory acceptance while reducing federal funding and time requirements. (author)

  6. A Comparison of Types of Robot Control for Programming by Demonstration

    DEFF Research Database (Denmark)

    Fischer, Kerstin; Kirstein, Franziska; Jensen, Lars Christian;

    2016-01-01

    Programming by Demonstration (PbD) is an efficient way for non-experts to teach new skills to a robot. PbD can be carried out in different ways, for instance, by kinesthetic guidance, teleoperation or by using external controls. In this paper, we compare these three ways of controlling a robot...

  7. A Case Study of Technical Assistance to Demonstration Programs for Young Handicapped Children. Part II.

    Science.gov (United States)

    Behr, Shirley K.; And Others

    A project to provide technical assistance to staffs of two demonstration programs for preschool handicapped children and their families is described. The focus was technical assistance developed and exercised by the Technical Assistance Development System (TADS), which assisted in the development of the Handicapped Children's Early Education…

  8. A Case Study of Technical Assistance to Demonstration Programs for Young Handicapped Children. Part I.

    Science.gov (United States)

    Behr, Shirley K.; And Others

    The interim report presents an evaluation of TADS (Technical Assistance Development System) through case studies of TADS technical assistance to the staffs of two demonstration programs for preschool handicapped children and their families. Purposes of the study included obtaining indepth descriptions of the technical assistance process to…

  9. Startup, testing, and operation of the Santa Clara 2MW direct carbonate fuel cell demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    Skok, A.J.; Leo, A.J. [Fuel Cell Engineering Corp., Danbury, CT (United States); O`Shea, T.P. [Santa Clara Demonstration Project, CA (United States)

    1996-12-31

    The Santa Clara Demonstration Project (SCDP) is a collaboration between several utility organizations, Fuel Cell Engineering Corporation (FCE), and the U.S. Dept. Of Energy aimed at the demonstration of Energy Research Corporation`s (ERC) direct carbonate fuel cell (DFC) technology. ERC has been pursuing the development of the DFC for commercialization near the end of this decade, and this project is an integral part of the ERC commercialization effort. The objective of the Santa Clara Demonstration Project is to provide the first full, commercial scale demonstration of this technology. The approach ERC has taken in the commercialization of the DFC is described in detail elsewhere. An aggressive core technology development program is in place which is focused by ongoing interaction with customers and vendors to optimize the design of the commercial power plant. ERC has selected a 2.85 MW power plant unit for initial market entry. Two ERC subsidiaries are supporting the commercialization effort: the Fuel Cell Manufacturing Corporation (FCMC) and the Fuel Cell Engineering Corporation (FCE). FCMC manufactures carbonate stacks and multi-stack modules, currently from its production facility in Torrington, CT. FCE is responsible for power plant design, integration of all subsystems, sales/marketing, and client services. FCE is serving as the prime contractor for the design, construction, and testing of the SCDP Plant. FCMC has manufactured the multi-stack submodules used in the DC power section of the plant. Fluor Daniel Inc. (FDI) served as the architect-engineer subcontractor for the design and construction of the plant and provided support to the design of the multi-stack submodules. FDI is also assisting the ERC companies in commercial power plant design.

  10. NASA solar dynamic ground test demonstration (GTD) program and its application to space nuclear power

    Science.gov (United States)

    Harper, William B.; Shaltens, Richard K.

    1993-01-01

    Closed Brayton cycle power conversion systems are readily adaptable to any heat source contemplated for space application. The inert gas working fluid can be used directly in gas-cooled reactors and coupled to a variety of heat sources (reactor, isotope or solar) by a heat exchanger. This point is demonstrated by the incorporation in the NASA 2 kWe Solar Dynamic (SD) Space Power Ground Test Demonstration (GTD) Program of the turboalternator-compressor and recuperator from the Brayton Isotope Power System (BIPS) program. This paper will review the goals and status of the SD GTD Program, initiated in April 1992. The performance of the BIPS isotope-heated system will be compared to the solar-heated GTD system incorporating the BIPS components and the applicability of the GTD test bed to dynamics space nuclear power R&D will be discussed.

  11. Intertechnology Corporation proposed test and evaluation plan, commercial buildings. National Solar Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    1976-09-01

    This report has three major parts. The first of these derives the requirements for the Test and Evaluation plan from the System Level Plan which is summarized in Section II. The second part contains the proposed plan to fill these requirements and includes hardware and software recommendations as well as procedures and management considerations. Primary emphasis has been given to the remote site because this is the area in which the commercial part of the demonstration is most unique. Finally, some pre-demonstration activities are described. The pilot program is intended to resolve a number of issues which arose in the course of the T and E plan. These relate to choice of scan frequencies, compression algorithms, etc. It is also intended to confirm performance and cost effectiveness of the site data collection package. The base line measurements of attitudes, etc. provide a reference mark against which one can measure the non-technical effectiveness of the demonstration program. (WDM)

  12. A data-gathering method for use in modeling energy research, development and demonstration programs

    Science.gov (United States)

    Meyer, M. A.; Booker, J. M.; Cullingford, H. S.; Peaslee, A. T., Jr.

    The development and testing of a data-gathering method for use in a computer program designed to model energy research, development, and demonstration programs for decisionmakers are described. The data-gathering method consists of face-to-face interviews with the scientists working on the projects that will be modeled by the computer program. The basic information gained from an interview includes time estimates for reaching certain project goals and the probability of achieving those goals within the times estimated. The interview method is based on decision analysis techniques. The Magnetic Fusion Energy program of the US Department of Energy was selected as the test case. The data gathering method was used at five fusion projects to determine whether it could meet its design criteria. Extensive statistical analysis was performed to learn how much the expert's answers agreed, what factors were likely to enter into their estimates, and how their estimates corresponded.

  13. Alternative fuels for vehicles fleet demonstration program final report. Volume 1: Summary

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The Alternative Fuels for Vehicles Fleet Demonstration Program (AFV-FDP) was a multiyear effort to collect technical data for use in determining the costs and benefits of alternative-fuel vehicles in typical applications in New York State. During 3 years of collecting data, 7.3 million miles of driving were accumulated, 1,003 chassis-dynamometer emissions tests were performed, 862,000 gallons of conventional fuel were saved, and unique information was developed about garage safety recommendations, vehicle performance, and other topics. Findings are organized by vehicle and fuel type. For light-duty compressed natural gas (CNG) vehicles, technology has evolved rapidly and closed-loop, electronically-controlled fuel systems provide performance and emissions advantages over open-loop, mechanical systems. The best CNG technology produces consistently low tailpipe emissions versus gasoline, and can eliminate evaporative emissions. Reduced driving range remains the largest physical drawback. Fuel cost is low ($/Btu) but capital costs are high, indicating that economics are best with vehicles that are used intensively. Propane produces impacts similar to CNG and is less expensive to implement, but fuel cost is higher than gasoline and safety codes limit use in urban areas. Light-duty methanol/ethanol vehicles provide performance and emissions benefits over gasoline with little impact on capital costs, but fuel costs are high. Heavy-duty CNG engines are evolving rapidly and provide large reductions in emissions versus diesel. Capital costs are high for CNG buses and fuel efficiency is reduced, but the fuel is less expensive and overall operating costs are about equal to those of diesel buses. Methanol buses provide performance and emissions benefits versus diesel, but fuel costs are high. Other emerging technologies were also evaluated, including electric vehicles, hybrid-electric vehicles, and fuel cells.

  14. DEMONSTRATION OF FUEL CELLS TO RECOVER ENERGY FROM ANAEROBIC DIGESTER GAS - PHASE I. CONCEPTUAL DESIGN, PRELIMINARY COST, AND EVALUATION STUDY

    Science.gov (United States)

    The report discusses Phase I (a conceptual design, preliminary cost, and evaluation study) of a program to demonstrate the recovery of energy from waste methane produced by anaerobic digestion of waste water treatment sludge. The fuel cell is being used for this application becau...

  15. 1986 fuel cell seminar: Program and abstracts

    Energy Technology Data Exchange (ETDEWEB)

    None

    1986-10-01

    Ninety nine brief papers are arranged under the following session headings: gas industry's 40 kw program, solid oxide fuel cell technology, phosphoric acid fuel cell technology, molten carbonate fuel cell technology, phosphoric acid fuel cell systems, power plants technology, fuel cell power plant designs, unconventional fuels, fuel cell application and economic assessments, and plans for commerical development. The papers are processed separately for the data base. (DLC)

  16. DOE's Innovative Treatment Remediation Demonstration Program accelerating the implementation of innovative technologies

    International Nuclear Information System (INIS)

    A program to help accelerate the adoption and implementation of new and innovative remediation technologies has been initiated by the Department of Energy's (DOE) Environmental Restoration Program Office (EM40). Developed as a Public-Private Partnership program in cooperation with the US Environmental Protection Agency's (EPA) Technology Innovation Office (TIO) and coordinated by Sandia National Laboratories, the Innovative Treatment Remediation Demonstration (ITRD) Program attempts to reduce many of the classic barriers to the use of new technologies by involving government, industry, and regulatory agencies in the assessment, implementation, and validation of innovative technologies. In this program, DOE facilities work cooperatively with EPA, industry, national laboratories, and state and federal regulatory agencies to establish remediation demonstrations using applicable innovative technologies at their sites. Selected innovative technologies are used to remediate small, one to two acre, sites to generate the full-scale and real-world operating, treatment performance, and cost data needed to validate these technologies and gain acceptance by industry and regulatory agencies, thus accelerating their use nationwide. Each ITRD project developed at a DOE site is designed to address a typical soil or groundwater contamination issue facing both DOE and industry. This includes sites with volatile organic compound (VOC), semi-VOC, heavy metal, explosive residue, and complex or multiple constituent contamination. Projects are presently underway at three DOE facilities, while additional projects are under consideration for initiation in FY96 at several additional DOE sites. A brief overview of the ITRD Program, program plans, and the status and progress of existing ITRD projects are reviewed in this paper

  17. Programmed Cell Death During Caenorhabditis elegans Development.

    Science.gov (United States)

    Conradt, Barbara; Wu, Yi-Chun; Xue, Ding

    2016-08-01

    Programmed cell death is an integral component of Caenorhabditis elegans development. Genetic and reverse genetic studies in C. elegans have led to the identification of many genes and conserved cell death pathways that are important for the specification of which cells should live or die, the activation of the suicide program, and the dismantling and removal of dying cells. Molecular, cell biological, and biochemical studies have revealed the underlying mechanisms that control these three phases of programmed cell death. In particular, the interplay of transcriptional regulatory cascades and networks involving multiple transcriptional regulators is crucial in activating the expression of the key death-inducing gene egl-1 and, in some cases, the ced-3 gene in cells destined to die. A protein interaction cascade involving EGL-1, CED-9, CED-4, and CED-3 results in the activation of the key cell death protease CED-3, which is tightly controlled by multiple positive and negative regulators. The activation of the CED-3 caspase then initiates the cell disassembly process by cleaving and activating or inactivating crucial CED-3 substrates; leading to activation of multiple cell death execution events, including nuclear DNA fragmentation, mitochondrial elimination, phosphatidylserine externalization, inactivation of survival signals, and clearance of apoptotic cells. Further studies of programmed cell death in C. elegans will continue to advance our understanding of how programmed cell death is regulated, activated, and executed in general. PMID:27516615

  18. Early Site Permit Demonstration Program: Plant parameters envelope report. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1993-03-01

    The Early Site Permit (ESP) Demonstration Program is the nuclear industry`s initiative for piloting the early resolution of siting-related issues before the detailed design proceedings of the combined operating license review. The ESP Demonstration Program consists of three phases. The plant parameters envelopes task is part of Phase 1, which addresses the generic review of applicable federal regulations and develops criteria for safety and environmental assessment of potential sites. The plant parameters envelopes identify parameters that characterize the interface between an ALWR design and a potential site, and quantify the interface through values selected from the Utility Requirements Documents, vendor design information, or engineering assessments. When augmented with site-specific information, the plant parameters envelopes provide sufficient information to allow ESPs to be granted based on individual ALWR design information or enveloping design information for the evolutionary, passive, or generic ALWR plants. This document is expected to become a living document when used by future applicants.

  19. Rio Grande Erosion Potential Demonstration - Report for the National Border Technology Program; TOPICAL

    International Nuclear Information System (INIS)

    This demonstration project is a collaboration among DOE, Sandia National Laboratories, the University of Texas, El Paso (UTEP), the International Boundary and Water Commission (IBWC), and the US Army Corps of Engineers (USACE). Sandia deployed and demonstrated a field measurement technology that enables the determination of erosion and transport potential of sediments in the Rio Grande. The technology deployed was the Mobile High Shear Stress Flume. This unique device was developed by Sandia's Carlsbad Programs for the USACE and has been used extensively in collaborative efforts on near shore and river systems throughout the United States. Since surface water quantity and quality along with human health is an important part of the National Border Technology Program, technologies that aid in characterizing, managing, and protecting this valuable resource from possible contamination sources is imperative

  20. City of Las Vegas Plug-in Hybrid Electric Vehicle Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-12-31

    The City of Las Vegas was awarded Department of Energy (DOE) project funding in 2009, for the City of Las Vegas Plug-in Hybrid Electric Vehicle Demonstration Program. This project allowed the City of Las Vegas to purchase electric and plug-in hybrid electric vehicles and associated electric vehicle charging infrastructure. The City anticipated the electric vehicles having lower overall operating costs and emissions similar to traditional and hybrid vehicles.

  1. Cobalt-60 heat source demonstration program. Phase III. Fabrication. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1973-06-01

    Significant accomplishments completed during Phase III of the /sup 60/Co Heat Source Demonstration program include the following: encapsulation of 2 MCi of /sup 60/Co; fabrication of the heat source, including the ASME coded pressure vessel/core assembly, and biological shielding; endurance testing of a prototype heat pipe for a period of 28 months; fabrication and qualification of the heat pipe emergency cooling subsystem; issue of the safety evaluation report, reference 3, and the operations manual, reference 4; and heat source assembly. The planned demonstration test program was modified to include testing of a total power system. Based on an evaluation of available power conversion systems, which included the closed-cycle Brayton and organic Rankine systems, the closed-cycle Brayton system was selected for use. Selection was based on advantages offered by the direct coupling of this conversion system with the gas-cooled heat source. In implementing the test program, the AiResearch BCD power conversion system was to be coupled to the heat source following initial heat source performance testing and part way through the endurance test. In accordance with the program redirection the following Phase IV checkout operations were completed to evaluate procedural and hardware acceptability: heat source dummy fueling; fueling cask sielding survey; and heat source shielding survey (single pin). Completion of these latter activities verified the acceptability of critical characteristics of the heat source and its supporting equipment.

  2. Demonstrating a small utility approach to demand-side program implementation

    International Nuclear Information System (INIS)

    The US DOE awarded a grant to the Burlington Electric Department (B.E.D.) to test a demand-side management (DSM) demonstration program designed to quickly save a significant amount of power with little disruption to the utility's customers or its normal operations. B.E.D. is a small municipal utility located in northern Vermont, with a lengthy history of successful DSM involvement. In our grant application, we proposed to develop a replicable program and approach to DSM that might be useful to other small utilities and to write a report to enable such replication. We believe that this DSM program and/or individual program components are replicable. This report is designed to allow other utilities interested in DSM to replicate this program or specific program design features to meet their DSM goals. We also wanted to use the opportunity of this grant to test the waters of residential heating fuel-switching. We hoped to test the application of one fuel-switching technology, and to benefit from the lessons learned in developing a full-scale DSM program for this end- use. To this end the pilot effort has been very successful. In the pilot pressure we installed direct-vent gas fired space heaters sized as supplemental heating units in 44 residences heated solely by electric resistance heat. We installed the gas space heating units at no cost to the owners or residents. We surveyed participating customers. The results of those surveys are included in this report and preliminary estimates of winter peak capacity load reductions are also noted in this report

  3. Demonstrating a small utility approach to demand-side program implementation

    Energy Technology Data Exchange (ETDEWEB)

    1991-10-01

    The US DOE awarded a grant to the Burlington Electric Department (B.E.D.) to test a demand-side management (DSM) demonstration program designed to quickly save a significant amount of power with little disruption to the utility's customers or its normal operations. B.E.D. is a small municipal utility located in northern Vermont, with a lengthy history of successful DSM involvement. In our grant application, we proposed to develop a replicable program and approach to DSM that might be useful to other small utilities and to write a report to enable such replication. We believe that this DSM program and/or individual program components are replicable. This report is designed to allow other utilities interested in DSM to replicate this program or specific program design features to meet their DSM goals. We also wanted to use the opportunity of this grant to test the waters of residential heating fuel-switching. We hoped to test the application of one fuel-switching technology, and to benefit from the lessons learned in developing a full-scale DSM program for this end- use. To this end the pilot effort has been very successful. In the pilot pressure we installed direct-vent gas fired space heaters sized as supplemental heating units in 44 residences heated solely by electric resistance heat. We installed the gas space heating units at no cost to the owners or residents. We surveyed participating customers. The results of those surveys are included in this report and preliminary estimates of winter peak capacity load reductions are also noted in this report.

  4. Industrial Fuel Gas Demonstration Plant Program. Task III, Demonstration plant safety, industrial hygiene, and major disaster plan (Deliverable No. 35)

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-03-01

    This Health and Safety Plan has been adopted by the IFG Demonstration Plant managed by Memphis Light, Gas and Water at Memphis, Tennessee. The plan encompasses the following areas of concern: Safety Plan Administration, Industrial Health, Industrial Safety, First Aid, Fire Protection (including fire prevention and control), and Control of Safety Related Losses. The primary objective of this plan is to achieve adequate control of all potentially hazardous activities to assure the health and safety of all employees and eliminate lost work time to both the employees and the company. The second objective is to achieve compliance with all Federal, state and local laws, regulations and codes. Some thirty specific safe practice instruction items are included.

  5. Status of the demonstration irradiation program of the new fuel bundle CANFLEX-NU in Korea

    International Nuclear Information System (INIS)

    In the late part of 1999, the Korea Electric Power Corporation has initiated a program CANFLEX-NU (Natural Uranium) fuel in the Wolsong Generating Station (WGS) - no.1 which has been operating since 1983, because the CANFLEX could be used to recover some of a CANDU heat transport system operation margins that had decreased due to The Korea Ministry of Science and Technology (MOST) has recognized the successful demonstration irradiation of 24 CANFLEX bundles at the Pt. Lepreau Generating Station in Canada, as final verification of the CANFLEX design in preparation for full core conversion. Therefore, MOST has pushed and gave a financial support to a KEPRI/KAERI Joint Industrialization Program of CANFLEX-NU Fuel, which will be for 3 years from 2000 November, to validate CANFLEX-NU fuel bundle performance in direct conditions of relevance under the Korean licensing requirements as well as to evaluate the fuel fabrication capability, and to produce a safety analysis report for the full-core implementation. The economic benefits of CANFLEX-NU fuel are directly dependent on the thermalhydraulic performance. Switching from the existing 37-element fuel to the CANFLEX fuel will be largely driven by the economic benefits to be realized. Showing a positive result in the economic evaluation as well as successfully demonstrating the CANFLEX fuel irradiation in WGS-no. 1, the full-core implementation of the fuel at the WGS-no.1 in Korea will proceed by starting the licensing process at around 2003 April because the safety report for the full-core conversion will be ready by 2003 March. This paper describes the status of CANFLEX-NU fuel industrialization program in Korea, as well as the fuel design features. It summarizes the plan of CANFLEX-NU fuel demonstration irradiation at the WGS-no. 1 in Korea and the status of documentation for the demonstration irradiation as well as for the CANFLEX-NU full-core implementation. (author)

  6. Programmed Cell Death in Unicellular Phytoplankton.

    Science.gov (United States)

    Bidle, Kay D

    2016-07-11

    Unicellular, planktonic, prokaryotic and eukaryotic photoautotrophs (phytoplankton) have an ancient evolutionary history on Earth during which time they have played key roles in the regulation of marine food webs, biogeochemical cycles, and Earth's climate. Since they represent the basis of aquatic ecosystems, the manner in which phytoplankton die critically determines the flow and fate of photosynthetically fixed organic matter (and associated elements), ultimately constraining nutrient flow. Programmed cell death (PCD) and associated pathway genes, which are triggered by a variety of abiotic (nutrient, light, osmotic) and biotic (virus infection, allelopathy) environmental stresses, have an integral grip on cell fate, and have shaped the ecological success and evolutionary trajectory of diverse phytoplankton lineages. A combination of physiological, biochemical, and genetic techniques in model algal systems has demonstrated a conserved molecular and mechanistic framework of stress surveillance, signaling, and death activation pathways, involving collective and coordinated participation of organelles, redox enzymes, metabolites, and caspase-like proteases. This mechanistic understanding has provided insight into the integration of sensing and transduction of stress signals into cellular responses, and the mechanistic interfaces between PCD, cell stress and virus infection pathways. It has also provided insight into the evolution of PCD in unicellular photoautotrophs, the impact of PCD on the fate of natural phytoplankton assemblages and its role in aquatic biogeochemical cycles. PMID:27404255

  7. Programmed cell death and cell extrusion in rat duodenum

    DEFF Research Database (Denmark)

    Schauser, Kirsten; Larsson, Lars-Inge

    2005-01-01

    The small intestinal epithelium is continously renewed through a balance between cell division and cell loss. How this balance is achieved is uncertain. Thus, it is unknown to what extent programmed cell death (PCD) contributes to intestinal epithelial cell loss. We have used a battery of techniq...

  8. The relationship of fluidized bed technology to the U.S. Clean Coal Technology demonstration program

    International Nuclear Information System (INIS)

    Fluidized Bed Combustion projects (both AFBCs and PFBCs) have a prominent role in the US DOE Clean Coal Technology (CCT) Program. This program has the successful commercialization of these technologies as its primary objective and this is the basic criterion for government funding and participation in the development and demonstration of the technologies. Under the CCT program the US DOE is actively involved in the development and operation of three Fluidized Bed Technology projects, NUCLA, TIDD, and SPORN, and is in the negotiation stage on others, Dairyland, Nichols and Tallahassee. All of these projects, along with the operating information on fluidized beds in the industrial sector, will provide a basis for evaluating future utilization of Fluidized Bed Technology in the market place. Impacting upon further utilization will be the time-frame and the Clean Air Act Amendments of 1990. This paper presents the results of a study to ascertain the commercial readiness of Fluidized Bed Technology to meet the emissions and time-frame requirements of the Clean Air Act Amendments of 1990. Specifically addressed are: Commercialization criteria/factors which candidate and/or existing CCTs must achieve in order to gain market acceptance. The status of Fluidized Bed Technology in achieving these commercialization criteria for market acceptance (industrial and utility) consistent with the time frame of the Clean Air Act Amendments of 1990. Recommendations of commercialization criteria for future fluidized bed CCT demonstration projects

  9. Fast Cycled Magnet demonstrator program at CERN: Instrumentation and measurement campaign

    CERN Document Server

    Willering, G; Borgnolutti, F; Bottura, L; Datskov, V; Deferne, G; Feuvrier, J; Fiscarelli, L; Giloux, C; Guinchard, M; Roger, V

    2013-01-01

    In an effort to develop economical magnets for an upgrade of the LHC injector complex, CERN started an R&D program on superconducting Fast Cycled Magnets (FCM) in 2009. One of the challenges in this program was to develop a test station, which started working in summer 2012 when the FCM dipole demonstrator was tested. The magnet contains several important features, like forced-flow cooling of supercritical He and it has a protection scheme based direct voltage measurement with co-wound voltage tap wires. In this paper we report on the cryogenic and powering requirements and operation, the quench protection system, the temperature and mechanical measurements. The functioning of the test station and instrumentation are evaluated and we will discuss the measurements on a detailed level.

  10. Isotope Brayton ground demonstration testing and flight qualification. Volume 1. Technical program

    Energy Technology Data Exchange (ETDEWEB)

    1974-12-09

    A program is proposed for the ground demonstration, development, and flight qualification of a radioisotope nuclear heated dynamic power system for use on space missions beginning in the 1980's. This type of electrical power system is based upon and combines two aerospace technologies currently under intense development; namely, the MHW isotope heat source and the closed Brayton cycle gas turbine. This power system represents the next generation of reliable, efficient economic electrical power equipment for space, and will be capable of providing 0.5 to 2.0 kW of electric power to a wide variety of spacecraft for earth orbital and interplanetary missions. The immediate design will be based upon the requirements for the Air Force SURVSATCOM mission. The proposal is presented in three volumes plus an Executive Summary. This volume describes the tasks in the technical program.

  11. Criticality safety analyses for the Spent-Fuel Handling and Packaging Program Demonstration

    International Nuclear Information System (INIS)

    The major process steps performed to place the spent-fuel assemblies into storage were described to provide a perspective of the overall operation conducted at E-MAD. Using both numerical calculations and published data, it is concluded that no single accident can result in the achievement of criticality. Based on the evaluation of postulated double accident conditions, it is concluded that no double accident having any reasonable probability of occurrence can result in criticality at the E-MAD site. Therefore, all proposed fuel handling and storage activities in the Spent-Fuel Handling and Packaging Program Demonstration meet basic nuclear criticality safety standards. 9 figures

  12. CLIMATE CHANGE FUEL CELL PROGRAM

    Energy Technology Data Exchange (ETDEWEB)

    Steven A. Gabrielle

    2004-12-03

    This report discusses the first year of operation of a fuel cell power plant located at the Sheraton Edison Hotel, Edison, New Jersey. PPL EnergyPlus, LLC installed the plant under a contract with the Starwood Hotels & Resorts Worldwide, Inc. A DFC{reg_sign}300 fuel cell, manufactured by FuelCell Energy, Inc. of Danbury, CT was selected for the project. The fuel cell successfully operated from June 2003 to May 2004. This report discusses the performance of the plant during this period.

  13. Spent nuclear fuel removal program at the West Valley Demonstration Project: Topical report

    Energy Technology Data Exchange (ETDEWEB)

    Connors, B. J.; Golden, M. P.; Valenti, P. J.; Winkel, J. J.

    1987-03-01

    The spent nuclear fuel removal program at the West Valley Demonstration Project (WVDP) consisted of removing the spent nuclear fuel (SNF) assemblies from the storage pool in the plant, loading them in shielded casks, and preparing the casks for transportation. So far, four fuel removal campaigns have been completed with the return of 625 spent nuclear fuel assemblies to their four utility owners. A fifth campaign, which is not yet completed, will transfer the remaining 125 fuel assemblies to a government site in Idaho. A spent fuel rod consolidation demonstration has been completed, and the storage canisters and their racks are being removed from the fuel receiving and storage pool to make way for installation of the size reduction equipment. A brief history of the West Valley reprocessing plant and the events leading to the storage and ownership of the spent nuclear fuel assemblies and their subsequent removal from West Valley are also recorded as background information. 3 refs., 16 figs., 9 tabs.

  14. Spent nuclear fuel removal program at the West Valley Demonstration Project: Topical report

    International Nuclear Information System (INIS)

    The spent nuclear fuel removal program at the West Valley Demonstration Project (WVDP) consisted of removing the spent nuclear fuel (SNF) assemblies from the storage pool in the plant, loading them in shielded casks, and preparing the casks for transportation. So far, four fuel removal campaigns have been completed with the return of 625 spent nuclear fuel assemblies to their four utility owners. A fifth campaign, which is not yet completed, will transfer the remaining 125 fuel assemblies to a government site in Idaho. A spent fuel rod consolidation demonstration has been completed, and the storage canisters and their racks are being removed from the fuel receiving and storage pool to make way for installation of the size reduction equipment. A brief history of the West Valley reprocessing plant and the events leading to the storage and ownership of the spent nuclear fuel assemblies and their subsequent removal from West Valley are also recorded as background information. 3 refs., 16 figs., 9 tabs

  15. Multiple Curricula for B Cell Developmental Programming.

    Science.gov (United States)

    Rothenberg, Ellen V

    2016-09-20

    B-1 B cells differ from conventional B-2 B cells functionally, but how these differences relate to the ontogeny of these lineages has been unclear. Two recent Immunity articles, Kristiansen et al. (2016) and Montecino-Rodriguez et al. (2016), now provide insight into the origins of B-1 and B-2 B cells, revealing a multi-layered developmental program and successive waves of B cell precursors.

  16. Multiple Curricula for B Cell Developmental Programming.

    Science.gov (United States)

    Rothenberg, Ellen V

    2016-09-20

    B-1 B cells differ from conventional B-2 B cells functionally, but how these differences relate to the ontogeny of these lineages has been unclear. Two recent Immunity articles, Kristiansen et al. (2016) and Montecino-Rodriguez et al. (2016), now provide insight into the origins of B-1 and B-2 B cells, revealing a multi-layered developmental program and successive waves of B cell precursors. PMID:27653594

  17. Integrated Demonstration program -- Applying innovative technologies to VOC remediation in arid environments

    International Nuclear Information System (INIS)

    Historical activities at US DOE facilities around the United States during World War II, including development of a nuclear deterrent, resulted in the discharge of chemical and radioactive materials to the environment. The DOE has now focused a major technical effort on the mitigation of the effects of those discharges through an environmental restoration program. The DOE Office of Technology Development (OTD) launched a major initiative to develop and demonstrate more cost-effective and safer means of restoring contaminated areas. This initiative integrates available conventional technologies and innovative and/or emerging technologies with ongoing environmental restoration activities. The ultimate goal of the OTD initiative is to have demonstrated technologies available for commercial application when they are needed at DOE sites. A key element of the initiative is the creation of Integrated Demonstrations (IDs). The VOC Arid-ID is providing the mechanism for developing and deploying cradle-to-grave technical solutions using a creative approach for solving environmental cleanup problems at the Hanford site. Within the last 10 months, and from it's inception, the ID has been successful in deploying demonstrated technologies from monitoring and characterization through site remediation. The creation of IDs has provided DOE with a mechanism to accelerate the Hanford site cleanup by bringing technology forward in a timely manner to meet the needs of the stakeholders

  18. Non-Flow-Through Fuel Cell System Test Results and Demonstration on the SCARAB Rover

    Science.gov (United States)

    Scheidegger, Brianne, T.; Burke, Kenneth A.; Jakupca, Ian J.

    2012-01-01

    This paper describes the results of the demonstration of a non-flow-through PEM fuel cell as part of a power system on the SCARAB rover. A 16-cell non-flow-through fuel cell stack from Infinity Fuel Cell and Hydrogen, Inc. was incorporated into a power system designed to act as a range extender by providing power to the rover s hotel loads. This work represents the first attempt at a ground demonstration of this new technology aboard a mobile test platform. Development and demonstration were supported by the Office of the Chief Technologist s Space Power Systems Project and the Advanced Exploration System Modular Power Systems Project.

  19. Northwest Hazardous Waste Research, Development, and Demonstration Center: Program Plan. [Contains glossary

    Energy Technology Data Exchange (ETDEWEB)

    1988-02-01

    The Northwest Hazardous Waste Research, Development, and Demonstration Center was created as part of an ongoing federal effort to provide technologies and methods that protect human health and welfare and environment from hazardous wastes. The Center was established by the Superfund Amendments and Reauthorization Act (SARA) to develop and adapt innovative technologies and methods for assessing the impacts of and remediating inactive hazardous and radioactive mixed-waste sites. The Superfund legislation authorized $10 million for Pacific Northwest Laboratory to establish and operate the Center over a 5-year period. Under this legislation, Congress authorized $10 million each to support research, development, and demonstration (RD and D) on hazardous and radioactive mixed-waste problems in Idaho, Montana, Oregon, and Washington, including the Hanford Site. In 1987, the Center initiated its RD and D activities and prepared this Program Plan that presents the framework within which the Center will carry out its mission. Section 1.0 describes the Center, its mission, objectives, organization, and relationship to other programs. Section 2.0 describes the Center's RD and D strategy and contains the RD and D objectives, priorities, and process to be used to select specific projects. Section 3.0 contains the Center's FY 1988 operating plan and describes the specific RD and D projects to be carried out and their budgets and schedules. 9 refs., 18 figs., 5 tabs.

  20. Lawrence Livermore National Laboratory's Atomic Vapor Laser Isotope Separation program: laser technology and demonstration facilities

    International Nuclear Information System (INIS)

    The Atomic Vapor Laser Isotope Separation (AVLIS) program at Lawrence Livermore National Laboratory (LLNL) is developing a large-scale process for photophysically enriching isotopes of several elements. The program now deploys plant prototypical hardware to demonstrate this enrichment process at full scale. The Laser Demonstration Facility, a fully integrated laser system has been constructed and when complete will produce more than 5000 W of tunable average power at a pulse repetition frequency >10 kHz. In AVLIS, an atomic vapor of mixed isotopes is irradiated with laser light precisely tuned to photoionize only desired isotopes. The resulting electrically charged photoions are deflected in an electric field to collector (or extractor) plates where they condense as enriched products. The AVLIS laser system consists of a series of high-average-power dye lasers optically pumped by an array of copper lasers. The dye lasers are used to do the three-step photoionization process. These lasers are well suited for the AVLIS process for both technical and economic reasons. They provide the precise narrowband (<100-MHz), short-pulse (<100-nsec), high-average-power, high-repetition-frequency laser light required by the atomic spectroscopy and vapor flow properties. Equally important, the capital and operating costs of this laser system in its present state of development are consistent with a commercially viable process deployment

  1. Climate Change Fuel Cell Program

    Energy Technology Data Exchange (ETDEWEB)

    Paul Belard

    2006-09-21

    Verizon is presently operating the largest Distributed Generation Fuel Cell project in the USA. Situated in Long Island, NY, the power plant is composed of seven (7) fuel cells operating in parallel with the Utility grid from the Long Island Power Authority (LIPA). Each fuel cell has an output of 200 kW, for a total of 1.4 mW generated from the on-site plant. The remaining power to meet the facility demand is purchased from LIPA. The fuel cell plant is utilized as a co-generation system. A by-product of the fuel cell electric generation process is high temperature water. The heat content of this water is recovered from the fuel cells and used to drive two absorption chillers in the summer and a steam generator in the winter. Cost savings from the operations of the fuel cells are forecasted to be in excess of $250,000 per year. Annual NOx emissions reductions are equivalent to removing 1020 motor vehicles from roadways. Further, approximately 5.45 million metric tons (5 millions tons) of CO2 per year will not be generated as a result of this clean power generation. The project was partially financed with grants from the New York State Energy R&D Authority (NYSERDA) and from Federal Government Departments of Defense and Energy.

  2. Programmed Cell Death in Neurospora crassa

    Directory of Open Access Journals (Sweden)

    A. Pedro Gonçalves

    2014-01-01

    Full Text Available Programmed cell death has been studied for decades in mammalian cells, but simpler organisms, including prokaryotes, plants, and fungi, also undergo regulated forms of cell death. We highlight the usefulness of the filamentous fungus Neurospora crassa as a model organism for the study of programmed cell death. In N. crassa, cell death can be triggered genetically due to hyphal fusion between individuals with different allelic specificities at het loci, in a process called “heterokaryon incompatibility.” Chemical induction of cell death can also be achieved upon exposure to death-inducing agents like staurosporine, phytosphingosine, or hydrogen peroxide. A summary of the recent advances made by our and other groups on the discovery of the mechanisms and mediators underlying the process of cell death in N. crassa is presented.

  3. Results of 200 KW fuel cell evaluation programs

    Energy Technology Data Exchange (ETDEWEB)

    Torrey, J.M.; Merten, G.P. [SAIC, San Diego, CA (United States); Binder, M.J. [Army Construction Engineering Research Labs., Champaign, IL (United States)] [and others

    1996-12-31

    Science Applications International Corporation (SAIC) has installed six monitoring systems on ONSI Corporation 200 kW phosphoric acid fuel cells. Three of the systems were installed for the U.S. Army Construction Engineering Research Laboratories (USACERL) which is coordinating the Department of Defense (DoD) fuel cell Demonstration Program and three were installed under a contract with the New York State Energy Research and Development Authority (NYSERDA). Monitoring of the three NYSERDA sites has been completed. Monitoring systems for the DoD fuel cells were installed in August, 1996 and thus no operating data was available at the time of this writing, but will be presented at the Fuel Cell Seminar. This paper will present the monitoring configuration and research approach for each program. Additionally, summary performance data is presented for the completed NYSERDA program.

  4. Clean Coal Technology Demonstration Program: Project fact sheets 2000, status as of June 30, 2000

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-09-01

    The Clean Coal Technology Demonstration Program (CCT Program), a model of government and industry cooperation, responds to the Department of Energy's (DOE) mission to foster a secure and reliable energy system that is environmentally and economically sustainable. The CCT Program represents an investment of over $5.2 billion in advanced coal-based technology, with industry and state governments providing an unprecedented 66 percent of the funding. With 26 of the 38 active projects having completed operations, the CCT Program has yielded clean coal technologies (CCTs) that are capable of meeting existing and emerging environmental regulations and competing in a deregulated electric power marketplace. The CCT Program is providing a portfolio of technologies that will assure that U.S. recoverable coal reserves of 274 billion tons can continue to supply the nation's energy needs economically and in an environmentally sound manner. As the nation embarks on a new millennium, many of the clean coal technologies have realized commercial application. Industry stands ready to respond to the energy and environmental demands of the 21st century, both domestically and internationally, For existing power plants, there are cost-effective environmental control devices to control sulfur dioxide (S02), nitrogen oxides (NO,), and particulate matter (PM). Also ready is a new generation of technologies that can produce electricity and other commodities, such as steam and synthetic gas, and provide efficiencies and environmental performance responsive to global climate change concerns. The CCT Program took a pollution prevention approach as well, demonstrating technologies that remove pollutants or their precursors from coal-based fuels before combustion. Finally, new technologies were introduced into the major coal-based industries, such as steel production, to enhance environmental performance. Thanks in part to the CCT Program, coal--abundant, secure, and economical

  5. Uptake during an oral cholera vaccine pilot demonstration program, Odisha, India.

    Science.gov (United States)

    Kar, Shantanu K; Pach, Alfred; Sah, Binod; Kerketta, Anna S; Patnaik, Bikash; Mogasale, VijayaLaxmi; Kim, Yang Hee; Rath, Shyam Bandhu; Shin, Sunheang; Khuntia, Hemant K; Bhattachan, Anuj; Puri, Mahesh K; Wierzba, Thomas F; Kaljee, Linda M

    2014-01-01

    Approximately 30% of reported global cholera cases occur in India. In 2011, a household survey was conducted 4 months after an oral cholera vaccine pilot demonstration project in Odisha India to assess factors associated with vaccine up-take and exposure to a communication and social mobilization campaign. Nine villages were purposefully selected based on socio-demographics and demonstration participation rates. Households were stratified by level of participation and randomly selected. Bivariate and ordered logistic regression analyses were conducted. 517/600 (86%) selected households were surveyed. At the household level, participant compared to non-participant households were more likely to use the local primary health centers for general healthcare (P communication, at the household level verbal communication was reported to be more useful than written communication. However written communication was perceived to be more useful by respondents in low-participating villages compared to average-participating villages (p = 0.007) These data on participation in an oral cholera vaccine demonstration program are important in light of the World Health Organization's (WHO) recommendations for pre-emptive use of cholera vaccine among vulnerable populations in endemic settings. Continued research is needed to further delineate barriers to vaccine up-take within and across targeted communities in low- and middle-income countries. PMID:25483631

  6. Grid-Optimization Program for Photovoltaic Cells

    Science.gov (United States)

    Daniel, R. E.; Lee, T. S.

    1986-01-01

    CELLOPT program developed to assist in designing grid pattern of current-conducting material on photovoltaic cell. Analyzes parasitic resistance losses and shadow loss associated with metallized grid pattern on both round and rectangular solar cells. Though performs sensitivity studies, used primarily to optimize grid design in terms of bus bar and grid lines by minimizing power loss. CELLOPT written in APL.

  7. 34 CFR 425.1 - What is the Demonstration Projects for the Integration of Vocational and Academic Learning Program?

    Science.gov (United States)

    2010-07-01

    ... of Vocational and Academic Learning Program? 425.1 Section 425.1 Education Regulations of the Offices... EDUCATION DEMONSTRATION PROJECTS FOR THE INTEGRATION OF VOCATIONAL AND ACADEMIC LEARNING PROGRAM General § 425.1 What is the Demonstration Projects for the Integration of Vocational and Academic...

  8. 48 CFR 52.219-19 - Small Business Concern Representation for the Small Business Competitiveness Demonstration Program.

    Science.gov (United States)

    2010-10-01

    ... Representation for the Small Business Competitiveness Demonstration Program. 52.219-19 Section 52.219-19 Federal... Representation for the Small Business Competitiveness Demonstration Program. As prescribed in 19.1008(a), insert the following provision: Small Business Concern Representation for the Small Business...

  9. Fuel Cell Seminar, 1992: Program and abstracts

    Energy Technology Data Exchange (ETDEWEB)

    1992-12-31

    This year`s theme, ``Fuel Cells: Realizing the Potential,`` focuses on progress being made toward commercial manufacture and use of fuel cell products. Fuel cell power plants are competing for market share in some applications and demonstrations of market entry power plants are proceeding for additional applications. Development activity on fuel cells for transportation is also increasing; fuel cell products have potential in energy and transportation industries, with very favorable environmental impacts. This Seminar has the purpose of fostering communication by providing a forum for the international community interested in development, application, and business opportunities related fuel cells. Over 190 technical papers are included, the majority being processed for the data base.

  10. Final Technical Report: Residential Fuel Cell Demonstration by the Delaware County Electric Cooperative, Inc.

    Energy Technology Data Exchange (ETDEWEB)

    Mark Hilson Schneider

    2007-06-06

    This demonstration project contributes to the knowledge base in the area of fuel cells in stationary applications, propane fuel cells, edge-of-grid applications for fuel cells, and energy storage in combination with fuel cells. The project demonstrated that it is technically feasible to meet the whole-house electrical energy needs of a typical upstate New York residence with a 5-kW fuel cell in combination with in-home energy storage without any major modifications to the residence or modifications to the consumption patterns of the residents of the home. The use of a fuel cell at constant output power through a 120-Volt inverter leads to system performance issues including: • relatively poor power quality as quantified by the IEEE-defined short term flicker parameter • relatively low overall system efficiency Each of these issues is discussed in detail in the text of this report. The fuel cell performed well over the 1-year demonstration period in terms of availability and efficiency of conversion from chemical energy (propane) to electrical energy at the fuel cell output terminals. Another strength of fuel cell performance in the demonstration was the low requirements for maintenance and repair on the fuel cell. The project uncovered a new and important installation consideration for propane fuel cells. Alcohol added to new propane storage tanks is preferentially absorbed on the surface of some fuel cell reformer desulfurization filters. The experience on this project indicates that special attention must be paid to the volume and composition of propane tank additives. Size, composition, and replacement schedules for the de-sulfurization filter bed should be adjusted to account for propane tank additives to avoid sulfur poisoning of fuel cell stacks. Despite good overall technical performance of the fuel cell and the whole energy system, the demonstration showed that such a system is not economically feasible as compared to other commercially available

  11. Experimental and Demonstration Program, Copper Country Intermediate School District. End of Budget Period Reports 1972 and 1973.

    Science.gov (United States)

    Michigan State Dept. of Education, Lansing.

    End of budget period reports are presented for an experimental and demonstration program aimed at increasing the learning process and achievement of K-4 children and to effect internal and external change in teachers' attitudes and behavior. The program, instituted in a rural, low income area, consists of an Orff music program and a theater…

  12. 75 FR 27808 - Section 8 Housing Choice Voucher Program-Demonstration Project of Small Area Fair Market Rents in...

    Science.gov (United States)

    2010-05-18

    ... are to determine payment standard amounts for the Housing Choice Voucher (HCV) program, to determine... the flexibilities available in the voucher program, to set voucher payment standards at varying and... URBAN DEVELOPMENT Section 8 Housing Choice Voucher Program--Demonstration Project of Small Area...

  13. General Motors automotive fuel cell program

    Energy Technology Data Exchange (ETDEWEB)

    Fronk, M.H.

    1995-08-01

    The objectives of the second phase of the GM/DOE fuel cell program is to develop and test a 30 kW fuel cell powerplant. This powerplant will be based on a methanol fuel processor and a proton exchange membrane PM fuel cell stack. In addition, the 10 kW system developed during phase I will be used as a {open_quotes}mule{close_quotes} to test automotive components and other ancillaries, needed for transient operation.

  14. Programmed cell death during quinoa perisperm development

    OpenAIRE

    López-Fernández, María Paula; Maldonado, Sara

    2013-01-01

    At seed maturity, quinoa (Chenopodium quinoa Willd.) perisperm consists of uniform, non-living, thin-walled cells full of starch grains. The objective of the present study was to study quinoa perisperm development and describe the programme of cell death that affects the entire tissue. A number of parameters typically measured during programmed cell death (PCD), such as cellular morphological changes in nuclei and cytoplasm, endoreduplication, DNA fragmentation, and the participation of nucle...

  15. Apoptosis: A Review of Programmed Cell Death

    OpenAIRE

    Elmore, Susan

    2007-01-01

    The process of programmed cell death, or apoptosis, is generally characterized by distinct morphological characteristics and energy-dependent biochemical mechanisms. Apoptosis is considered a vital component of various processes including normal cell turnover, proper development and functioning of the immune system, hormone-dependent atrophy, embryonic development and chemical-induced cell death. Inappropriate apoptosis (either too little or too much) is a factor in many human conditions incl...

  16. Programmed cell death and hybrid incompatibility.

    Science.gov (United States)

    Frank, S A; Barr, C M

    2003-01-01

    We propose a new theory to explain developmental aberrations in plant hybrids. In our theory, hybrid incompatibilities arise from imbalances in the mechanisms that cause male sterility in hermaphroditic plants. Mitochondria often cause male sterility by killing the tapetal tissue that nurtures pollen mother cells. Recent evidence suggests that mitochondria destroy the tapetum by triggering standard pathways of programmed cell death. Some nuclear genotypes repress mitochondrial male sterility and restore pollen fertility. Normal regulation of tapetal development therefore arises from a delicate balance between the disruptive effects of mitochondria and the defensive countermeasures of the nuclear genes. In hybrids, incompatibilities between male-sterile mitochondria and nuclear restorers may frequently upset the regulatory control of programmed cell death, causing tapetal abnormalities and male sterility. We propose that hybrid misregulation of programmed cell death may also spill over into other tissues, explaining various developmental aberrations observed in hybrids.

  17. Alternative fuels for vehicles fleet demonstration program. Final report, volume 2: Appendices

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-06-01

    The Alternative Fuels for Vehicles Fleet Demonstration Program (AFV-FDP) was a multiyear effort to collect technical data for use in determining the costs and benefits of alternative-fuel vehicles (AFVs) in typical applications in New York State. This report, Volume 2, includes 13 appendices to Volume 1 that expand upon issues raised therein. Volume 1 provides: (1) Information about the purpose and scope of the AFV-FDP; (2) A summary of AFV-FDP findings organized on the basis of vehicle type and fuel type; (3) A short review of the status of AFV technology development, including examples of companies in the State that are active in developing AFVs and AFV components; and (4) A brief overview of the status of AFV deployment in the State. Volume 3 provides expanded reporting of AFV-FDP technical details, including the complete texts of the brochure Garage Guidelines for Alternative Fuels and the technical report Fleet Experience Survey Report, plus an extensive glossary of AFV terminology. The appendices cover a wide range of issues including: emissions regulations in New York State; production and health effects of ozone; vehicle emissions and control systems; emissions from heavy-duty engines; reformulated gasoline; greenhouse gases; production and characteristics of alternative fuels; the Energy Policy Act of 1992; the Clean Fuel Fleet Program; garage design guidelines for alternative fuels; surveys of fleet managers using alternative fuels; taxes on conventional and alternative fuels; and zero-emission vehicle technology.

  18. DC-9 Flight Demonstration Program with Refanned JT8D Engines. Volume 3; Performance and Analysis

    Science.gov (United States)

    1975-01-01

    The JT8D-109 engine has a sea level static, standard day bare engine takeoff thrust of 73,840 N. At sea level standard day conditions the additional thrust of the JT8D-109 results in 2,040 kg additional takeoff gross weight capability for a given field length. Range loss of the DC-9 Refan airplane for long range cruise was determined. The Refan airplane demonstrated stall, static longitudinal stability, longitudinal control, longitudinal trim, minimum control speeds, and directional control characteristics similar to the DC-9-30 production airplane and complied with airworthiness requirements. Cruise, climb, and thrust reverser performance were evaluated. Structural and dynamic ground test, flight test and analytical results substantiate Refan Program requirements that the nacelle, thrust reverser hardware, and the airplane structural modifications are flightworthy and certifiable and that the airplane meets flutter speed margins. Estimated unit cost of a DC-9 Refan retrofit program is 1.338 million in mid-1975 dollars with about an equal split in cost between airframe and engine.

  19. Support and power plant documentation for the gas turbine powered bus demonstration program

    Science.gov (United States)

    Nigro, D. N.; Stewart, R. G.; Apple, S. A.

    1982-01-01

    The operational experience obtained for the GT404-4 gas turbine engines in the intercity and intracity Bus Demonstration Programs is described for the period January 1980 through September 1981. Support for the engines and automatic transmissions involved in this program provided engineering and field service, spare parts and tools, training, and factory overhauls. the Greyhound (intercity) coaches accumulated 183,054 mi (294,595 km) and 5154 hr of total operation. The Baltimore Transit (intracity) coaches accumulated 40,567 mi (65,285 km) and 1840 hr of total operation. In service, the turbine powered Greyhound and Transit coaches achieved approximately 25% and 40% lower fuel mileage, respectively, than did the production diesel powered coaches. The gas turbine engine will require the advanced ceramic development currently being sponsored by the DOE and NASA to achieve fuel economy equivalent not only to that of today's diesel engines but also to the projected fuel economy of the advanced diesel engines of the 1990s. Sufficient experience was not achieved with the coaches prior to the start of service to identify and eliminate many of the problems associated with the startup of new equipment. Because of these problems, the mean miles between incident were unacceptably low. The future gas turbine system should be developed sufficiently to establish satisfactory durability prior to evaluation in revenue service. Commercialization of the gas turbine bus engine remains a viable goal for the future.

  20. Occupational Safety and Health Program at the West Valley Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Calderon

    1999-04-30

    The West Valley Nuclear Services Co. LLC (WVNS) is committed to provide a safe, clean, working environment for employees, and to implement U.S. Department of Energy (DOE) requirements affecting worker safety. The West Valley Demonstration Project (WVDP) Occupational Safety and Health Program is designed to protect the safety, health, and well-being of WVDP employees by identifying, evaluating, and controlling biological, chemical, and physical hazards in the work place. Hazards are controlled within the requirements set forth in the reference section at the end of this report. It is the intent of the WVDP Occupational Safety and Health Program to assure that each employee is provided with a safe and healthy work environment. This report shows the logical path toward ensuring employee safety in planning work at the WVDP. In general, planning work to be performed safely includes: combining requirements from specific programs such as occupational safety, industrial hygiene, radiological control, nuclear safety, fire safety, environmental protection, etc.; including WVDP employees in the safety decision-making processes; pre-planning using safety support re-sources; and integrating the safety processes into the work instructions. Safety management principles help to define the path forward for the WVDP Occupational Safety and Health Program. Roles, responsibilities, and authority of personnel stem from these ideals. WVNS and its subcontractors are guided by the following fundamental safety management principles: ''Protection of the environment, workers, and the public is the highest priority. The safety and well-being of our employees, the public, and the environment must never be compromised in the aggressive pursuit of results and accomplishment of work product. A graded approach to environment, safety, and health in design, construction, operation, maintenance, and deactivation is incorporated to ensure the protection of the workers, the public, and the

  1. Occupational Safety and Health Program at the West Valley Demonstration Project

    International Nuclear Information System (INIS)

    The West Valley Nuclear Services Co. LLC (WVNS) is committed to provide a safe, clean, working environment for employees, and to implement U.S. Department of Energy (DOE) requirements affecting worker safety. The West Valley Demonstration Project (WVDP) Occupational Safety and Health Program is designed to protect the safety, health, and well-being of WVDP employees by identifying, evaluating, and controlling biological, chemical, and physical hazards in the work place. Hazards are controlled within the requirements set forth in the reference section at the end of this report. It is the intent of the WVDP Occupational Safety and Health Program to assure that each employee is provided with a safe and healthy work environment. This report shows the logical path toward ensuring employee safety in planning work at the WVDP. In general, planning work to be performed safely includes: combining requirements from specific programs such as occupational safety, industrial hygiene, radiological control, nuclear safety, fire safety, environmental protection, etc.; including WVDP employees in the safety decision-making processes; pre-planning using safety support re-sources; and integrating the safety processes into the work instructions. Safety management principles help to define the path forward for the WVDP Occupational Safety and Health Program. Roles, responsibilities, and authority of personnel stem from these ideals. WVNS and its subcontractors are guided by the following fundamental safety management principles: ''Protection of the environment, workers, and the public is the highest priority. The safety and well-being of our employees, the public, and the environment must never be compromised in the aggressive pursuit of results and accomplishment of work product. A graded approach to environment, safety, and health in design, construction, operation, maintenance, and deactivation is incorporated to ensure the protection of the workers, the public, and the environment

  2. Specific insulin binding in bovine chromaffin cells; demonstration of preferential binding to adrenalin-storing cells

    Energy Technology Data Exchange (ETDEWEB)

    Serck-Hanssen, G.; Soevik, O.

    1987-12-28

    Insulin binding was studied in subpopulations of bovine chromaffin cells enriched in adrenalin-producing cells (A-cells) or noradrenalin-producing cells (NA-cells). Binding of /sup 125/I-insulin was carried out at 15/sup 0/C for 3 hrs in the absence or presence of excess unlabeled hormone. Four fractions of cells were obtained by centrifugation on a stepwise bovine serum albumin gradient. The four fractions were all shown to bind insulin in a specific manner and the highest binding was measured in the cell layers of higher densities, containing mainly A-cells. The difference in binding of insulin to the four subpopulations of chromaffin cells seemed to be related to differences in numbers of receptors as opposed to receptor affinities. The authors conclude that bovine chromaffin cells possess high affinity binding sites for insulin and that these binding sites are mainly confined to A-cells. 24 references, 2 figures, 1 table.

  3. Recovery Act. Solid Oxide Fuel Cell Diesel Auxilliary Power Unit Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Geiger, Gail E. [Delphi Automotive Systems, LLC., Gillingham (United Kingdom)

    2013-09-30

    Solid Oxide Fuel Cell Diesel Auxilliary Power Unit Demonstration Project. Summarizing development of Delphi’s next generation SOFC system as the core power plant to prove the viability of the market opportunity for a 3-5 kW diesel SOFC system. Report includes test and demonstration results from testing the diesel APU in a high visibility fleet customer vehicle application.

  4. Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential.

    Science.gov (United States)

    Nazarov, Igor; Lee, Jae W; Soupene, Eric; Etemad, Sara; Knapik, Derrick; Green, William; Bashkirova, Elizaveta; Fang, Xiaohui; Matthay, Michael A; Kuypers, Frans A; Serikov, Vladimir B

    2012-05-01

    We describe human chorionic mesenchymal stem cell (hCMSC) lines obtained from the chorion of human term placenta with high therapeutic potential in human organ pathology. hCMSCs propagated for more than 100 doublings without a decrease in telomere length and with no telomerase activity. Cells were highly positive for the embryonic stem cell markers OCT-4, NANOG, SSEA-3, and TRA-1-60. In vitro, cells could be differentiated into neuron-like cells (ectoderm), adipocytes, osteoblasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm)-derivatives of all three germ layers. hCMSCs effectively facilitated repair of injured epithelium as demonstrated in an ex vivo-perfused human lung preparation injured by Escherichia coli endotoxin and in in vitro human lung epithelial cultures. We conclude that the chorion of human term placenta is an abundant source of multipotent stem cells that are promising candidates for cell-based therapies. PMID:23197815

  5. Programmed cell death in cereal aleurone.

    Science.gov (United States)

    Fath, A; Bethke, P; Lonsdale, J; Meza-Romero, R; Jones, R

    2000-10-01

    Progress in understanding programmed cell death (PCD) in the cereal aleurone is described. Cereal aleurone cells are specialized endosperm cells that function to synthesize and secrete hydrolytic enzymes that break down reserves in the starchy endosperm. Unlike the cells of the starchy endosperm, aleurone cells are viable in mature grain but undergo PCD when germination is triggered or when isolated aleurone layers or protoplasts are incubated in gibberellic acid (GA). Abscisic acid (ABA) slows down the process of aleurone cell death and isolated aleurone protoplasts can be kept alive in media containing ABA for up to 6 months. Cell death in barley aleurone occurs only after cells become highly vacuolated and is manifested in an abrupt loss of plasma membrane integrity. Aleurone cell death does not follow the apoptotic pathway found in many animal cells. The hallmarks of apoptosis, including internucleosomal DNA cleavage, plasma membrane and nuclear blebbing and formation of apoptotic bodies, are not observed in dying aleurone cells. PCD in barley aleurone cells is accompanied by the accumulation of a spectrum of nuclease and protease activities and the loss of organelles as a result of cellular autolysis.

  6. Zero Emission Bay Area (ZEBA) Fuel Cell Bus Demonstration Results. Fourth Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2015-07-02

    This report presents results of a demonstration of fuel cell electric buses (FCEB) operating in Oakland, California. Alameda-Contra Costa Transit District (AC Transit) leads the Zero Emission Bay Area (ZEBA) demonstration, which includes 12 advanced-design fuel cell buses and two hydrogen fueling stations. The FCEBs in service at AC Transit are 40-foot, low-floor buses built by Van Hool with a hybrid electric propulsion system that includes a US Hybrid fuel cell power system and EnerDel lithium-based energy storage system. The buses began revenue service in May 2010.

  7. Photovoltaic Power System and Power Distribution Demonstration for the Desert RATS Program

    Science.gov (United States)

    Colozza, Anthony; Jakupca, Ian; Mintz, Toby; Herlacher, Mike; Hussey, Sam

    2012-01-01

    A stand alone, mobile photovoltaic power system along with a cable deployment system was designed and constructed to take part in the Desert Research And Technology Studies (RATS) lunar surface human interaction evaluation program at Cinder Lake, Arizona. The power system consisted of a photovoltaic array/battery system. It is capable of providing 1 kW of electrical power. The system outputs were 48 V DC, 110 V AC, and 220 V AC. A cable reel with 200 m of power cable was used to provide power from the trailer to a remote location. The cable reel was installed on a small trailer. The reel was powered to provide low to no tension deployment of the cable. The cable was connected to the 220 V AC output of the power system trailer. The power was then converted back to 110 V AC on the cable deployment trailer for use at the remote site. The Scout lunar rover demonstration vehicle was used to tow the cable trailer and deploy the power cable. This deployment was performed under a number of operational scenarios, manned operation, remote operation and tele-robotically. Once deployed, the cable was used to provide power, from the power system trailer, to run various operational tasks at the remote location.

  8. DOE complex buried waste characterization assessment. Buried Waste Integrated Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Kaae, P.S.; Holter, G.M.; Garrett, S.M.K.

    1993-01-01

    The work described in this report was conducted by Pacific Northwest Laboratory to provide information to the Buried Waste Integrated Demonstration (BWID) program. The information in this report is intended to provide a complex-wide planning base for th.e BWID to ensure that BWID activities are appropriately focused to address the range of remediation problems existing across the US Department of Energy (DOE) complex. This report contains information characterizing the 2.1 million m{sup 3} of buried and stored wastes and their associated sites at six major DOE facilities. Approximately 85% of this waste is low-level waste, with about 12% TRU or TRU mixed waste; the remaining 3% is low-level mixed waste. In addition, the report describes soil contamination sites across the complex. Some of the details that would be useful in further characterizing the buried wastes and contaminated soil sites across the DOE complex are either unavailable or difficult to locate. Several options for accessing this information and/or improving the information that is available are identified in the report. This document is a companion to Technology Needs for Remediation: Hanford and Other DOE Sites, PNL-8328 (Stapp 1993).

  9. SPACE-R Thermionic Space Nuclear Power System: Design and Technology Demonstration Program

    Science.gov (United States)

    1993-05-01

    This semiannual technical progress report summarizes the technical progress and accomplishments for the Thermionic Space Nuclear Power System (TI-SNPS) Design and Technology Demonstration Program of the prime contractor, Space Power Incorporated (SPI), its subcontractors, and supporting national laboratories during the first half of the government fiscal year (GFY) 1993. SPI's subcontractors and supporting national laboratories include: Babcock & Wilcox for the reactor core and externals; Space Systems/Loral for the spacecraft integration; Thermocore for the radiator heat pipes and the heat exchanger; INERTEK of CIS for the TFE, core elements, and nuclear tests; Argonne National Laboratories for nuclear safety, physics, and control verification; and Oak Ridge National laboratories for materials testing. Parametric trade studies are near completion. However, technical input from INERTEK has yet to be provided to determine some of the baseline design configurations. The INERTEK subcontract is expected to be initiated soon. The point design task has been initiated. The thermionic fuel element (TFE) is undergoing several design iterations. The reactor core vessel analysis and design has also been started.

  10. Bluetooth wireless monitoring, diagnosis and calibration interface for control system of fuel cell bus in Olympic demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Hua, Jianfeng; Lin, Xinfan; Xu, Liangfei; Li, Jianqiu; Ouyang, Minggao [Tsinghua University, State Key Laboratory of Automotive Safety and Energy, Beijing100084 (China)

    2009-01-15

    With the worldwide deterioration of the natural environment and the fossil fuel crisis, the possible commercialization of fuel cell vehicles has become a hot topic. In July 2008, Beijing started a clean public transportation plan for the 29th Olympic games. Three fuel cell city buses and 497 other low-emission vehicles are now serving the Olympic core area and Beijing urban areas. The fuel cell buses will operate along a fixed bus line for 1 year as a public demonstration of green energy vehicles. Due to the specialized nature of fuel cell engines and electrified power-train systems, measurement, monitoring and calibration devices are indispensable. Based on the latest Bluetooth wireless technology, a novel Bluetooth universal data interface was developed for the control system of the fuel cell city bus. On this platform, a series of wireless portable control auxiliary systems have been implemented, including wireless calibration, a monitoring system and an in-system programming platform, all of which are ensuring normal operation of the fuel cell buses used in the demonstration. (author)

  11. Bluetooth wireless monitoring, diagnosis and calibration interface for control system of fuel cell bus in Olympic demonstration

    Science.gov (United States)

    Hua, Jianfeng; Lin, Xinfan; Xu, Liangfei; Li, Jianqiu; Ouyang, Minggao

    With the worldwide deterioration of the natural environment and the fossil fuel crisis, the possible commercialization of fuel cell vehicles has become a hot topic. In July 2008, Beijing started a clean public transportation plan for the 29th Olympic games. Three fuel cell city buses and 497 other low-emission vehicles are now serving the Olympic core area and Beijing urban areas. The fuel cell buses will operate along a fixed bus line for 1 year as a public demonstration of green energy vehicles. Due to the specialized nature of fuel cell engines and electrified power-train systems, measurement, monitoring and calibration devices are indispensable. Based on the latest Bluetooth wireless technology, a novel Bluetooth universal data interface was developed for the control system of the fuel cell city bus. On this platform, a series of wireless portable control auxiliary systems have been implemented, including wireless calibration, a monitoring system and an in-system programming platform, all of which are ensuring normal operation of the fuel cell buses used in the demonstration.

  12. Realistic outcomes: lessons from community-based research and demonstration programs for the prevention of cardiovascular diseases.

    Science.gov (United States)

    Mittelmark, M B; Hunt, M K; Heath, G W; Schmid, T L

    1993-01-01

    Public health departments nation-wide are implementing community-based cardiovascular disease (CVD) prevention programs. Many such programs are turning for guidance to three research and demonstration projects: the Stanford Five City Project, the Pawtucket Heart Health Program, and the Minnesota Heart Health Program. This article summarizes some of the lessons learned in these projects and recommends strategies for the new generation of CVD prevention programs. The core of a successful program is the community organization process. This involves identification and activation of key community leaders, stimulation of citizens and organizations to volunteer time and offer resources to CVD prevention, and the promotion of prevention as a community theme. A wide range of intervention settings are available for health promotion. As is true for the workplace, places of worship are receptive to health promotion programs and have access to large numbers of people. Mass media are effective when used in conjunction with complementary messages delivered through other channels, such as school programs, adult education programs, and self-help programs. Community health professionals play a vital role in providing program endorsement and stimulating the participation of other community leaders. School-based programs promote long-term behavior change and reach beyond the school to actively involve parents. Innovative health promotion contests have widespread appeal and promote participation in other community interventions. In the area of evaluation, health program participation rates are appropriate primary outcome measures in most community-oriented prevention programs. Other program evaluation priorities include community analysis and formative evaluation, providing data to fine-tune interventions and define the needs and preferences of the community. It is premature to comment conclusively on the effectiveness of community-based CVD prevention programs in reducing population

  13. Tubular solid oxide fuel cell development program

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This paper presents an overview of the Westinghouse Solid Oxide Fuel Cell (SOFC) development activities and current program status. The Westinghouse goal is to develop a cost effective cell that can operate for 50,000 to 100,000 hours. Progress toward this goal will be discussed and test results presented for multiple single cell tests which have now successfully exceeded 56,000 hours of continuous power operation at temperature. Results of development efforts to reduce cost and increase power output of tubular SOFCs are described.

  14. National Program for Solar Heating and Cooling of Buildings. Project Date Summaries. Vol. I: Commercial and Residential Demonstrations.

    Science.gov (United States)

    Energy Research and Development Administration, Washington, DC. Div. of Solar Energy.

    Three volumes present brief abstracts of projects funded by the Energy Research and Development Administration (ERDA) and conducted under the National Program for Solar Heating and Cooling of Buildings through July 1976. The overall federal program includes demonstrations of heating and/or combined cooling for residential and commercial buildings…

  15. Effectiveness of a publicly-funded demonstration program to promote management of dryland salinity.

    Science.gov (United States)

    Robertson, M J; Measham, T G; Batchelor, G; George, R; Kingwell, R; Hosking, K

    2009-07-01

    Community and catchment-based approaches to salinity management continue to attract interest in Australia. In one such approach, Catchment Demonstration Initiative (CDI) projects were established by the Western Australian (WA) Government in 2000 for targeted investment in large-scale catchment-based demonstrations of integrated salinity management practices. The aim was to promote a process for technically-informed salinity management by landholders. This paper offers an evaluation of the effectiveness of one CDI project in the central wheatbelt of WA, covering issues including: its role in fostering adoption of salinity management options, the role of research and the technical requirements for design and implementation of on-ground works, the role of monitoring and evaluation, the identification and measurement of public and private benefits, comparison and identification of the place and value of plant-based and engineering-based options, reliance on social processes and impacts of constraints on capacity, management of governance and administration requirements and an appreciation of the value of group-based approaches. A number of factors may reduce the effectiveness of CDI-type approaches in facilitating landholder action to address salinity, many of these are socially-based. Such approaches can create considerable demands on landholders, can be expensive (because of the planning and accountability required) on the basis of dollars per hectare impacted, and can be difficult to garner ownership from all involved. An additional problem could be that few community groups would have the capacity to run such programs and disseminate the new knowledge so that the CDI-type projects can impact outside the focus catchment. In common with many publicly-funded approaches to salinity, we found that direct benefits on public assets are smaller than planned and that results from science-based requirements of monitoring and evaluation have long lead times, causing farmers

  16. Zero Emission Bay Area (ZEBA) Fuel Cell Bus Demonstration Results: Fifth Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, Leslie [National Renewable Energy Lab. (NREL), Golden, CO (United States); Post, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States); Jeffers, Matthew [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-06-01

    This report presents results of a demonstration of fuel cell electric buses (FCEB) operating in Oakland, California. Alameda-Contra Costa Transit District (AC Transit) leads the Zero Emission Bay Area (ZEBA) demonstration, which includes 13 advanced-design fuel cell buses and two hydrogen fueling stations. The ZEBA partners are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory (NREL) to evaluate the buses in revenue service. NREL has published four previous reports describing operation of these buses. This report presents new and updated results covering data from January 2015 through December 2015.

  17. Continued maturing of SOFC cell production technology and development and demonstration of SOFC stacks. Final report

    Energy Technology Data Exchange (ETDEWEB)

    2008-08-15

    The overall objective of the 6385 project was to develop stack materials, components and stack technology including industrial relevant manufacturing methods for cells components and stacks. Furthermore, the project should include testing and demonstration of the stacks under relevant operating conditions. A production of 6.829 cells, twenty 75-cell stacks and a number of small stacks was achieved. Major improvements were also made in the manufacturing methods and in stack design. Two test and demonstration activities were included in the project. The first test unit was established at H.C. OErsted power plant at the Copenhagen waterfront in order to perform test of SOFC stacks. The unit will be used for tests in other projects. The second demonstration unit is the alpha prototype demonstration in a system running on natural gas in Finland. The alpha prototype demonstration system with 24 TOFC (Topsoe Fuel Cell) stacks was established and started running in October 2007 and operational experience was gained in the period from October 2007 to February 2008. (auther)

  18. Chrysler Pentastar direct hydrogen fuel cell program

    Energy Technology Data Exchange (ETDEWEB)

    Kimble, M.; Deloney, D.

    1995-08-01

    The Chrysler Pentastar Electronics, Inc. Direct Hydrogen Fueled PEM Fuel Cell Hybrid Vehicle Program (DPHV) was initiated 1 July, 1994 with the following mission, {open_quotes}Design, fabricate, and test a Direct Hydrogen Fueled Proton Exchange Membrane (PEM) Fuel Cell System including onboard hydrogen storage, an efficient lightweight fuel cell, a gas management system, peak power augmentation and a complete system controls that can be economically mass produced and comply with all safety environmental and consumer requirements for vehicle applications for the 21st century.{close_quotes} The Conceptual Design for the entire system based upon the selection of an applicable vehicle and performance requirements that are consistent with the PNGV goals will be discussed. A Hydrogen Storage system that has been selected, packaged, and partially tested in accordance with perceived Hydrogen Safety and Infrastructure requirements will be discussed in addition to our Fuel Cell approach along with design of the {open_quotes}real{close_quotes} module. The Gas Management System and the Load Leveling System have been designed and the software programs have been developed and will be discussed along with a complete fuel cell test station that has the capability to test up to a 60 kW fuel cell system.

  19. The Solar Heating and Cooling Commercial Demonstration Program at Marshall Space Flight Center - Some problems and conclusions

    Science.gov (United States)

    Middleton, R. L.

    1978-01-01

    The origin and evolution of the Solar Heating and Cooling Commercial Demonstration Program by the Department of Energy and the Marshall Space Flight Center activities supporting this program from its conception are defined and discussed. Problems are summarized in the design and financial areas. It is concluded that the program has significantly assisted the creation of a viable solar testing and cooling industry. The cost effective procedures evolving from the program are expected to make a major contribution to reducing the effective life cycle cost of solar installation.

  20. National Program for Solar Heating and Cooling of Buildings. Project Data Summaries. Vol. II: Demonstration Support.

    Science.gov (United States)

    Energy Research and Development Administration, Washington, DC. Div. of Solar Energy.

    Brief abstracts of projects funded by the Energy Research and Development Administration (ERDA) and conducted under the National Program for Solar Heating and Cooling of Buildings are presented in three volumes. This, the second volume, identifies the major efforts currently underway in support of the national program. The National Aeronautics and…

  1. 77 FR 11677 - Medicaid Program; Review and Approval Process for Section 1115 Demonstrations

    Science.gov (United States)

    2012-02-27

    ... of the Social Security Act relating to Medicaid and the Children's Health Insurance Program (CHIP... 1115 of the Social Security Act (the Act) allows the Secretary of the Department of Health and Human... the Children's Health Insurance Program (CHIP) in title XXI of the Act in the same manner as...

  2. DOE Hydrogen and Fuel Cells Program Plan (September 2011)

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2011-09-01

    The Department of Energy Hydrogen and Fuel Cells Program Plan outlines the strategy, activities, and plans of the DOE Hydrogen and Fuel Cells Program, which includes hydrogen and fuel cell activities within the EERE Fuel Cell Technologies Program and the DOE offices of Nuclear Energy, Fossil Energy, and Science.

  3. Amnion-Epithelial-Cell-Derived Exosomes Demonstrate Physiologic State of Cell under Oxidative Stress.

    Science.gov (United States)

    Sheller, Samantha; Papaconstantinou, John; Urrabaz-Garza, Rheanna; Richardson, Lauren; Saade, George; Salomon, Carlos; Menon, Ramkumar

    2016-01-01

    At term, the signals of fetal maturity and feto-placental tissue aging prompt uterine readiness for delivery by transitioning quiescent myometrium to an active stage. It is still unclear how the signals reach the distant myometrium. Exosomes are a specific type of extracellular vesicle (EVs) that transport molecular signals between cells, and are released from a wide range of cells, including the maternal and fetal cells. In this study, we hypothesize that i) exosomes act as carriers of signals in utero-placental compartments and ii) exosomes reflect the physiologic status of the origin cells. The primary aims of this study were to determine exosomal contents in exosomes derived from primary amnion epithelial cells (AEC). We also determined the effect of oxidative stress on AEC derived exosomal cargo contents. AEC were isolated from amniotic membrane obtained from normal, term, not in labor placentae at delivery, and culture under standard conditions. Oxidative stress was induced using cigarette smoke extract for 48 hours. AEC-conditioned media were collected and exosomes isolated by differential centrifugations. Both growth conditions (normal and oxidative stress induced) produced cup shaped exosomes of around 50 nm, expressed exosomes enriched markers, such as CD9, CD63, CD81 and HSC70, embryonic stem cell marker Nanog, and contained similar amounts of cell free AEC DNA. Using confocal microscopy, the colocalization of histone (H) 3, heat shock protein (HSP) 70 and activated form of pro-senescence and term parturition associated marker p38 mitogen activated protein kinase (MAPK) (P-p38 MAPK) co-localized with exosome enrich marker CD9. HSP70 and P-p38 MAPK were significantly higher in exosomes from AEC grown under oxidative stress conditions than standard conditions (pmass spectrometry and bioinformatics analysis identified 221 different proteins involved in immunomodulatory response and cell-to-cell communication. This study determined AEC exosome

  4. DEMONSTRATION OF FUEL CELLS TO RECOVER ENERGY FROM LANDFILL GAS: PHASE II. PRETREATMENT SYSTEM PERFORMANCE MEASUREMENT

    Science.gov (United States)

    The report describes Phase II of a demonstration of the utilization of commercial phosphoric acid fuel cells to recover energy from landfill gas. This phase consisted primarily of the construction and testing of a Gas Pretreatment Unit (GPU) whose function is to remove those impu...

  5. Demonstration of Electrochemical Cell Properties by a Simple, Colorful Oxidation-reduction Experiment.

    Science.gov (United States)

    Hendricks, Lloyd J.; And Others

    1982-01-01

    Describes apparatus/methodology and provides background information for an experiment demonstrating electrochemical concepts and properties of electrochemical cells. The color of a solution close to an electrode is changed from that of the bulk solution to either of two contrasting colors depending on whether the reaction is oxidation or…

  6. Electrochemical Polishing of Silverware: A Demonstration of Voltaic and Galvanic Cells

    Science.gov (United States)

    Ivey, Michelle M.; Smith, Eugene T.

    2008-01-01

    In this demonstration, the students use their knowledge of electrochemistry to determine that tarnish can be removed from silverware by electrochemically converting it back to silver using items commonly available in the kitchen: aluminum foil and baking soda. In addition to using this system as an example of a galvanic cell, an electrolytic cell…

  7. Power conversion and quality of the Santa Clara 2 MW direct carbonate fuel cell demonstration plant

    Energy Technology Data Exchange (ETDEWEB)

    Skok, A.J. [Fuel Cell Engineering Corp., Danbury, CT (United States); Abueg, R.Z. [Basic Measuring Instruments, Santa Clara, CA (United States); Schwartz, P. [Fluor Daniel, Inc., Irvine, CA (United States)] [and others

    1996-12-31

    The Santa Clara Demonstration Project (SCDP) is the first application of a commercial-scale carbonate fuel cell power plant on a US electric utility system. It is also the largest fuel cell power plant ever operated in the United States. The 2MW plant, located in Santa Clara, California, utilizes carbonate fuel cell technology developed by Energy Research Corporation (ERC) of Danbury, Connecticut. The ultimate goal of a fuel cell power plant is to deliver usable power into an electrical distribution system. The power conversion sub-system does this for the Santa Clara Demonstration Plant. A description of this sub-system and its capabilities follows. The sub-system has demonstrated the capability to deliver real power, reactive power and to absorb reactive power on a utility grid. The sub-system can be operated in the same manner as a conventional rotating generator except with enhanced capabilities for reactive power. Measurements demonstrated the power quality from the plant in various operating modes was high quality utility grade power.

  8. Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour

    Science.gov (United States)

    Kazantseva, Jekaterina; Ivanov, Roman; Gasik, Michael; Neuman, Toomas; Hussainova, Irina

    2016-07-01

    Three-dimensional (3D) customized scaffolds capable to mimic a native extracellular matrix open new frontiers in cells manipulation and advanced therapy. The major challenge is in a proper substrate for in vitro models on engineered scaffolds, capable to modulate cells differentiation. Here for the first time we demonstrate novel design and functionality of the 3D porous scaffolds of aligned, self-assembled ceramic nanofibers of ultra-high anisotropy ratio (~107), augmented into graphene shells. This unique hybrid nano-network allows an exceptional combination of selective guidance stimuli of stem cells differentiation, immune reactions variations, and local immobilization of cancer cells, which was not available before. The scaffolds were shown to be able to direct human mesenchymal stem cells (important for stimulation of neuronal and muscle cells) preferential orientation, to suppress major inflammatory factors, and to localize cancer cells; all without additions of specific culture media. The selective downregulation of specific cytokines is anticipated as a new tool for understanding of human immune system and ways of treatment of associated diseases. The effects observed are self-regulated by cells only, without side effects, usually arising from use of external factors. New scaffolds may open new horizons for stem cells fate control such as towards axons and neurites regeneration (Alzheimer’s disease) as well as cancer therapy development.

  9. Amnion-Epithelial-Cell-Derived Exosomes Demonstrate Physiologic State of Cell under Oxidative Stress

    Science.gov (United States)

    Sheller, Samantha; Papaconstantinou, John; Urrabaz-Garza, Rheanna; Richardson, Lauren; Saade, George; Salomon, Carlos; Menon, Ramkumar

    2016-01-01

    At term, the signals of fetal maturity and feto-placental tissue aging prompt uterine readiness for delivery by transitioning quiescent myometrium to an active stage. It is still unclear how the signals reach the distant myometrium. Exosomes are a specific type of extracellular vesicle (EVs) that transport molecular signals between cells, and are released from a wide range of cells, including the maternal and fetal cells. In this study, we hypothesize that i) exosomes act as carriers of signals in utero-placental compartments and ii) exosomes reflect the physiologic status of the origin cells. The primary aims of this study were to determine exosomal contents in exosomes derived from primary amnion epithelial cells (AEC). We also determined the effect of oxidative stress on AEC derived exosomal cargo contents. AEC were isolated from amniotic membrane obtained from normal, term, not in labor placentae at delivery, and culture under standard conditions. Oxidative stress was induced using cigarette smoke extract for 48 hours. AEC-conditioned media were collected and exosomes isolated by differential centrifugations. Both growth conditions (normal and oxidative stress induced) produced cup shaped exosomes of around 50 nm, expressed exosomes enriched markers, such as CD9, CD63, CD81 and HSC70, embryonic stem cell marker Nanog, and contained similar amounts of cell free AEC DNA. Using confocal microscopy, the colocalization of histone (H) 3, heat shock protein (HSP) 70 and activated form of pro-senescence and term parturition associated marker p38 mitogen activated protein kinase (MAPK) (P-p38 MAPK) co-localized with exosome enrich marker CD9. HSP70 and P-p38 MAPK were significantly higher in exosomes from AEC grown under oxidative stress conditions than standard conditions (pexosome characteristics and their cargo reflected the physiologic status of the cell of origin and suggests that AEC-derived exosomal p38 MAPK plays a major role in determining the fate of pregnancy

  10. 2011 Annual Progress Report: DOE Hydrogen and Fuel Cells Program (Book)

    Energy Technology Data Exchange (ETDEWEB)

    2011-11-01

    In the past year, the DOE Hydrogen and Fuel Cells Program (the Program) made substantial progress toward its goals and objectives. The Program has conducted comprehensive and focused efforts to enable the widespread commercialization of hydrogen and fuel cell technologies in diverse sectors of the economy. With emphasis on applications that will effectively strengthen our nation's energy security and improve our stewardship of the environment, the Program engages in research, development, and demonstration of critical improvements in the technologies. Highlights of the Program's accomplishments can be found in the sub-program chapters of this report.

  11. US Polycrystalline Thin Film Solar Cells Program

    Science.gov (United States)

    Ullal, Harin S.; Zweibel, Kenneth; Mitchell, Richard L.

    1989-11-01

    The Polycrystalline Thin Film Solar Cells Program, part of the United States National Photovoltaic Program, performs R and D on copper indium diselenide and cadmium telluride thin films. The objective of the program is to support research to develop cells and modules that meet the U.S. Department of Energy's long-term goals by achieving high efficiencies (15 to 20 percent), low-cost ($50/m(sup 2)), and long-time reliability (30 years). The importance of work in this area is due to the fact that the polycrystalline thin-film CuInSe2 and CdTe solar cells and modules have made rapid advances. They have become the leading thin films for PV in terms of efficiency and stability. The U.S. Department of Energy has increased its funding through an initiative through the Solar Energy Research Institute in CuInSe2 and CdTe with subcontracts to start in spring 1990.

  12. US polycrystalline thin film solar cells program

    Energy Technology Data Exchange (ETDEWEB)

    Ullal, H S; Zweibel, K; Mitchell, R L [Solar Energy Research Inst., Golden, CO (USA)

    1989-11-01

    The Polycrystalline Thin Film Solar Cells Program, part of the United States National Photovoltaic Program, performs R D on copper indium diselenide and cadmium telluride thin films. The objective of the Program is to support research to develop cells and modules that meet the US Department of Energy's long-term goals by achieving high efficiencies (15%-20%), low-cost ($50/m{sup 2}), and long-time reliability (30 years). The importance of work in this area is due to the fact that the polycrystalline thin-film CuInSe{sub 2} and CdTe solar cells and modules have made rapid advances. They have become the leading thin films for PV in terms of efficiency and stability. The US Department of Energy has increased its funding through an initiative through the Solar Energy Research Institute in CuInSe{sub 2} and CdTe with subcontracts to start in Spring 1990. 23 refs., 5 figs.

  13. TNX GeoSiphon Cell (TGSC-1) Phase II Single Cell Deployment/Demonstration Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Phifer, M.A.

    1999-04-15

    This Phase II final report documents the Phase II testing conducted from June 18, 1998 through November 13, 1998, and it focuses on the application of the siphon technology as a sub-component of the overall GeoSiphon Cell technology. [Q-TPL-T-00004

  14. 75 FR 56946 - Medicaid Program; Review and Approval Process for Section 1115 Demonstrations

    Science.gov (United States)

    2010-09-17

    ... features. An estimate of the expected increase or decrease in annual aggregate expenditures by population... demonstration costs which would not otherwise be considered as expenditures under the Medicaid State plan, when... demonstration projects. In the September 27, 1994 Federal Register on (59 FR 49249), the Department of...

  15. 76 FR 67154 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Program

    Science.gov (United States)

    2011-10-31

    ... of the Secretary Science and Technology Reinvention Laboratory Personnel Management Demonstration... Register notice, 73 FR 73248-73252, to record amendments to eight legacy Science and Technology Reinvention Laboratory (STRL) Personnel Management Demonstration (demo) Project Plans resulting from section 1107(c)...

  16. Fast programming of peg-in-hole actions by human demonstration

    DEFF Research Database (Denmark)

    Yang, Yang; Lin, Linglong; Song, Y. T.;

    2014-01-01

    Peg-in-Hole actions play an important role in industrial assembly. In this paper, we present a system which performs such actions after learning by human demonstration. Strategies for getting robust human-demonstrated PiH trajectories are investigated, and a number of PiH experiments are conducte...

  17. Commercialization strategy for the Department of Energy's Solar Buildings Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    1981-01-01

    Efforts reported include: conducting a survey of existing commercial demonstration projects, categorizing these projects, determining criteria for evaluating the feasibility of continuing/aborting demonstration projects, and applying the criteria and making recommendations for which projects to continue and abort. It is recommended to refurbish 114 of the projects examined and to abandon 41. (LEW)

  18. The Clean Coal Technology Program 100 MWe demonstration of gas suspension absorption for flue gas desulfurization

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, F.E.; Hedenhag, J.G. [AirPol Inc., Teterboro, NJ (United States); Marchant, S.K.; Pukanic, G.W. [Dept. of Energy, Pittsburgh, PA (United States). Pittsburgh Energy Technology Center; Norwood, V.M.; Burnett, T.A. [Tennessee Valley Authority, Chattanooga, TN (United States)

    1997-12-31

    AirPol Inc., with the cooperation of the Tennessee Valley Authority (TVA) under a Cooperative Agreement with the United States Department of Energy, installed and tested a 10 MWe Gas Suspension Absorption (GSA) Demonstration system at TVA`s Shawnee Fossil Plant near Paducah, Kentucky. This low-cost retrofit project demonstrated that the GSA system can remove more than 90% of the sulfur dioxide from high-sulfur coal-fired flue gas, while achieving a relatively high utilization of reagent lime. This paper presents a detailed technical description of the Clean Coal Technology demonstration project. Test results and data analysis from the preliminary testing, factorial tests, air toxics texts, 28-day continuous demonstration run of GSA/electrostatic precipitator (ESP), and 14-day continuous demonstration run of GSA/pulse jet baghouse (PJBH) are also discussed within this paper.

  19. An analysis of the transition of the Objective Individual Combat Weapon (OICW) from advanced technology demonstration to acquisition program

    OpenAIRE

    Webb, Erik C.

    2002-01-01

    Approved for public release, distribution is unlimited The OICW is envisioned to be a lightweight, shoulder-fired weapon having a dual munitions capability and an advanced day/night fire control. The OICW is expected to provide substantial improvements in lethality over the predecessor rifle and carbine families of weapons. The Office of the Program Manager for Small Arms assessed the OICW Advanced Technology Demonstration process and program progress in 1998 and concluded the ATD process ...

  20. Simulated microgravity allows to demonstrate cell-to-cell communication in bacteria

    Science.gov (United States)

    Mastroleo, Felice; van Houdt, Rob; Mergeay, Max; Hendrickx, Larissa; Wattiez, Ruddy; Leys, Natalie

    Through the MELiSSA project, the European Space Agency aims to develop a closed life support system for oxygen, water and food production to support human life in space in forth-coming long term space exploration missions. This production is based on the recycling of the missions organic waste, including CO2 and minerals. The photosynthetic bacterium Rhodospir-illum rubrum S1H is used in MELiSSA to degrade organics with light energy and is the first MELiSSA organism that has been studied in space related environmental conditions (Mastroleo et al., 2009). It was tested in actual space flight to the International Space Station (ISS) as well as in ground simulations of ISS-like ionizing radiation and microgravity. In the present study, R. rubrum S1H was cultured in liquid medium in 2 devices simulating microgravity conditions, i.e. the Rotating Wall Vessel (RWV) and the Random Positioning Machine (RPM). The re-sponse of the bacterium was evaluated at both the transcriptomic and proteomic levels using respectively a dedicated whole-genome microarray and high-throughput gel-free quantitative proteomics. Both at transcriptomic and proteomic level, the bacterium showed a significant response to cultivation in simulated microgravity. The response to low fluid shear modeled microgravity in RWV was different than to randomized microgravity in RPM. Nevertheless, both tests pointed out a change in and a likely interrelation between cell-to-cell communica-tion (i.e. quorum sensing) and cell pigmentation (i.e. photosynthesis) for R. rubrum S1H in microgravity conditions. A new type of cell-to-cell communication molecule in R. rubrum S1H was discovered and characterized. It is hypothised that the lack of convection currents and the fluid quiescence in (simulated) microgravity limits communications molecules to be spread throughout the medium. Cultivation in this new artificial environment of simulated micro-gravity has showed new properties of this well know bacterium

  1. Eosinophilic enteritis demonstrated on 99mTc HMPAO white cell imaging

    International Nuclear Information System (INIS)

    Full text: A 46-year-old male with a 10-year history of biopsy proven Eosinophilic Gastroenteritis presented with a refractory exacerbation consisting of symptoms of severe lower abdominal pain, bloating and intermittent diarrhoea. Previous investigations during attacks had shown a blood eosinophilia, and earlier serial barium studies had demonstrated small bowel thickening and narrowing. The condition had previously responded to oral steroids. A 99mTc HMPAO labelled white cell scan was performed to assess for an inflammatory focus. The standard cell labelling technique was performed using 55ml of patient's whole blood, and labelled white cells reinjected approximately 2.5 hours later. Imaging was performed using a Siemens Ecam dual head gamma camera. Anterior, Posterior, right and left lateral images were acquired at 90 minutes and 3 hours post injection. All images were acquired using a 128W matrix for 5 minutes, with a 140kev peak and 20% window. Images demonstrated diffuse increased activity in the ileum on both the 90-minute and 3 hours images consistent with inflammatory changes. A recent barium swallow and follow through study was normal. The patient was successfully treated with a course of high dose intravenous steroids. Eosinophilic Enteritis is an extremely rare and poorly understood condition of unknown aetiology. This case study demonstrates that Nuclear Medicine white cell imaging may play a role in the diagnosis and management of patients diagnosed with this condition. Copyright (2002) The Australian and New Zealand Society of Nuclear Medicine Inc

  2. Evaluation of battery converters based on 4. 8-MW fuel cell demonstrator inverter. Final report. [Contains brief glossary

    Energy Technology Data Exchange (ETDEWEB)

    1980-10-01

    Electrical power conditioning is a critical element in the development of advanced electrochemical energy storage systems. This program evaluates the use of existing self-commutated converter technology (as developed by the Power Systems Division of United Technologies for the 4.8-MW Fuel Cell Demonstrator) with modification for use in battery energy storage systems. The program consists of three parts: evaluation of the cost and performance of a self-commutated converter modified to maintain production commonality between battery and fuel cell power conditioners, demonstration of the principal characteristics required for the battery application in MW-scale hardware, and investigation of the technical requirements of operation isolated from the utility system. A power-conditioning system consisting of a self-commutated converter augmented with a phase-controlled rectifier was selected and a preliminary design, prepared. A principal factor in this selection was production commonality with the fuel cell inverter system. Additional types of augmentation, and the use of a self-commutated converter system without augmentation, were also considered. A survey of advanced battery manufacturers was used to establish the dc interface characteristics. The principal characteristics of self-commutated converter operation required for battery application were demonstrated with the aid of an available 0.5-MW development system. A survey of five REA and municipal utilities and three A and E firms was conducted to determine technical requirements for operation in a mode isolated from the utility. Definitive requirements for this application were not established because of the limited scope of this study. 63 figures, 37 tables.

  3. NAS battery demonstration at American Electric Power:a study for the DOE energy storage program.

    Energy Technology Data Exchange (ETDEWEB)

    Newmiller, Jeff (Endecon Engineering, San Ramon, CA); Norris, Benjamin L. (Norris Energy Consulting Company, Martinez, CA); Peek, Georgianne Huff

    2006-03-01

    The first U.S. demonstration of the NGK sodium/sulfur battery technology was launched in August 2002 when a prototype system was installed at a commercial office building in Gahanna, Ohio. American Electric Power served as the host utility that provided the office space and technical support throughout the project. The system was used to both reduce demand peaks (peak-shaving operation) and to mitigate grid power disturbances (power quality operation) at the demonstration site. This report documents the results of the demonstration, provides an economic analysis of a commercial sodium/sulfur battery energy storage system at a typical site, and describes a side-by-side demonstration of the capabilities of the sodium/sulfur battery system, a lead-acid battery system, and a flywheel-based energy storage system in a power quality application.

  4. RDandD Programme 2010. Programme for research, development and demonstration of methods for the management and disposal of nuclear waste; Fud-program 2010. Program foer forskning, utveckling och demonstration av metoder foer hantering och slutfoervaring av kaernavfall

    Energy Technology Data Exchange (ETDEWEB)

    2010-09-15

    The RD and D programme 2010 gives an account of SKB's plans for research, development and demonstration during the period 2011-2016. SKB's activities are divided into two main areas - the programme for Low and Intermediate Level Waste (the Loma program) and the Nuclear Fuel Program. The RD and D Programme 2010 consists of five parts: Part I: Overall Plan, Part II: Loma program, Part III: Nuclear Fuel Program, Part IV: Research on analysis of long-term safety, Part V: Social Science Research. The 2007 RD and D programme was focused primarily on technology development to realize the final repository for spent nuclear fuel. The actions described were aimed at increasing awareness of long-term safety and to obtain technical data for application under the Nuclear Activities Act for the final repository for spent fuel and under the Environmental Code of the repository system. Many important results from these efforts are reported in this program. An overall account of the results will be given in the Licensing application in early 2011. The authorities' review of RD and D programme in 2007 and completion of the program called for clarification of plans and programs for the final repository for short-lived radioactive waste, SFR, and the final repository for waste, SFL. This RD and D program describes these plans in a more detailed way

  5. Zero Emission Bay Area (ZEBA) Fuel Cell Bus Demonstration: First Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K.; Eudy, L.

    2011-08-01

    This report documents the early implementation experience for the Zero Emission Bay Area (ZEBA) Demonstration, the largest fleet of fuel cell buses in the United States. The ZEBA Demonstration group includes five participating transit agencies: AC Transit (lead transit agency), Santa Clara Valley Transportation Authority (VTA), Golden Gate Transit (GGT), San Mateo County Transit District (SamTrans), and San Francisco Municipal Railway (Muni). The ZEBA partners are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory (NREL) to evaluate the buses in revenue service.

  6. 75 FR 15693 - Extension of Web-Based TRICARE Assistance Program Demonstration Project

    Science.gov (United States)

    2010-03-30

    ... (TRIAP), the other a permanent benefit (Telemental Health). Significant program changes days prior to... extension. SUMMARY: This notice is to advise interested parties of an extension to the Military Health... referenced in the original Federal Register (FR) Notice, 74 FR 3667, dated July 24, 2009. The...

  7. Cost-Effectiveness Analysis of Early Reading Programs: A Demonstration with Recommendations for Future Research

    Science.gov (United States)

    Hollands, Fiona M.; Kieffer, Michael J.; Shand, Robert; Pan, Yilin; Cheng, Henan; Levin, Henry M.

    2016-01-01

    We review the value of cost-effectiveness analysis for evaluation and decision making with respect to educational programs and discuss its application to early reading interventions. We describe the conditions for a rigorous cost-effectiveness analysis and illustrate the challenges of applying the method in practice, providing examples of programs…

  8. 77 FR 46439 - Medicare Program; Prior Authorization for Power Mobility Device (PMD) Demonstration

    Science.gov (United States)

    2012-08-03

    ... in 60-day and 30-day Federal Register notices that published on February 7, 2012 (77 FR 6124) and May 29, 2012 (77 FR 31616), respectively. The information collection requirements are approved under OMB... HUMAN SERVICES Centers for Medicare & Medicaid Services Medicare Program; Prior Authorization for...

  9. Toyota/Skyline Technical Education Network. Cooperative Demonstration Program. Final Performance Report.

    Science.gov (United States)

    Skyline Coll., San Bruno, CA.

    A joint project was conducted between Toyota Motor Sales and Skyline College (in the San Francisco, California, area) to create an automotive technician training program that would serve the needs of working adults. During the project, a model high technology curriculum suitable for adults was developed, the quality of instruction available for…

  10. Wildfire ignition resistant home design(WIRHD) program: Full-scale testing and demonstration final report.

    Energy Technology Data Exchange (ETDEWEB)

    Quarles, Stephen, L.; Sindelar, Melissa

    2011-12-13

    The primary goal of the Wildfire ignition resistant home design(WIRHD) program was to develop a home evaluation tool that could assess the ignition potential of a structure subjected to wildfire exposures. This report describes the tests that were conducted, summarizes the results, and discusses the implications of these results with regard to the vulnerabilities to homes and buildings.

  11. Statewide Demonstration of Not On Tobacco: A Gender-Sensitive Teen Smoking Cessation Program.

    Science.gov (United States)

    Dino, Geri A.; Horn, Kimberly A.; Goldcamp, Jennifer; Maniar, Sameep D.; Fernandes, Ancilla; Massey, Catherine J.

    2001-01-01

    Evaluated Not On Tobacco, a gender-sensitive, smoking cessation program for adolescents, comparing experimental group and brief intervention (BI) group students. Overall, Not On Tobacco females were four times more likely to quit than BI females. Approximately 84 percent of experimental group teens quit or reduced smoking, compared to 55 percent…

  12. SITE PROGRAM DEMONSTRATION OF THE SBP TECHNOLOGIES, INC. MEMBRANE FILTRATION SYSTEM ON CREOSOTE-CONTAMINATED WATER

    Science.gov (United States)

    The formed-in-place, membrane filtration system offered by SBP Technologies, Inc. of Stone Mountain, Georgia was evaluated by the U.S. EPA Superfund Inno- vative Technology Evaluation (SITE) Program. The evaluation lasted six days; ap- proximately 1000 gallons per day of water co...

  13. A Peer-Assisted Learning Program and Its Effect on Student Skill Demonstration

    Science.gov (United States)

    Carr, W. David; Volberding, Jennifer; Vardiman, Phillip

    2011-01-01

    Objective: To explore the effect of an intentional Peer-Assisted Learning (PAL) program on peer-tutors and peer-tutees for performance on specific psychomotor skills. Design and Setting: Randomized, pretest-posttest experimental design. Participants: Undergraduate students (N = 69, 42 females and 27 males, all participants were 18 to 22 years old,…

  14. Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN: an imaging demonstration

    Directory of Open Access Journals (Sweden)

    Yang ZS

    2014-03-01

    Full Text Available Zhuo-Shun Yang,1,* Xiang-Jun Tang,2,* Xing-Rong Guo,1 Dan-Dan Zou,1 Xu-Yong Sun,3 Jing-Bo Feng,1 Jie Luo,1 Long-Jun Dai,1,4 Garth L Warnock4 1Hubei Key Laboratory of Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China; 2Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China; 3Guangxi Key Laboratory for Transplant Medicine, 303 Hospital of PLA, Nanning, People’s Republic of China; 4Department of Surgery, University of British Columbia, Vancouver, BC, Canada *These authors contributed equally to this work Background: Mesenchymal stem cells (MSCs have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN engineering on MSCs’ capacity for cancer cell-oriented migration. Methods: MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system. Results: The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed. Conclusion: MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs’ tropism post-anticancer gene engineering. Keywords: gene therapy, mesenchymal stem cells, phosphatase and tensin homolog, cancer

  15. Simulation of Organic Solar Cells Using AMPS-1D Program

    Directory of Open Access Journals (Sweden)

    Samah G. Babiker

    2012-03-01

    Full Text Available The analysis of microelectronic and photonic structure in one dimension program [AMPS-1D] program has been successfully used to study inorganic solar cells. In this work the program has been used to optimize the performance of the organic solar cells. The cells considered consist of poly(2-methoxy-5-(3,7- dimethyloctyloxy-1,4-phenylenevinylene [MDMO-PPV

  16. Immunological Signatures after Bordetella pertussis Infection Demonstrate Importance of Pulmonary Innate Immune Cells

    Science.gov (United States)

    Brummelman, Jolanda; van der Maas, Larissa; Tilstra, Wichard; Pennings, Jeroen L. A.; Han, Wanda G. H.; van Els, Cécile A. C. M.; van Riet, Elly; Kersten, Gideon F. A.; Metz, Bernard

    2016-01-01

    Effective immunity against Bordetella pertussis is currently under discussion following the stacking evidence of pertussis resurgence in the vaccinated population. Natural immunity is more effective than vaccine-induced immunity indicating that knowledge on infection-induced responses may contribute to improve vaccination strategies. We applied a systems biology approach comprising microarray, flow cytometry and multiplex immunoassays to unravel the molecular and cellular signatures in unprotected mice and protected mice with infection-induced immunity, around a B. pertussis challenge. Pre-existing systemic memory Th1/Th17 cells, memory B-cells, and mucosal IgA specific for Ptx, Vag8, Fim2/3 were detected in the protected mice 56 days after an experimental infection. In addition, pre-existing high activity and reactivation of pulmonary innate cells such as alveolar macrophages, M-cells and goblet cells was detected. The pro-inflammatory responses in the lungs and serum, and neutrophil recruitment in the spleen upon an infectious challenge of unprotected mice were absent in protected mice. Instead, fast pulmonary immune responses in protected mice led to efficient bacterial clearance and harbored potential new gene markers that contribute to immunity against B. pertussis. These responses comprised of innate makers, such as Clca3, Retlna, Glycam1, Gp2, and Umod, next to adaptive markers, such as CCR6+ B-cells, CCR6+ Th17 cells and CXCR6+ T-cells as demonstrated by transcriptome analysis. In conclusion, besides effective Th1/Th17 and mucosal IgA responses, the primary infection-induced immunity benefits from activation of pulmonary resident innate immune cells, achieved by local pathogen-recognition. These molecular signatures of primary infection-induced immunity provided potential markers to improve vaccine-induced immunity against B. pertussis. PMID:27711188

  17. Test program to demonstrate the stability of hydrazine in propellant tanks

    Science.gov (United States)

    Moran, C. M.; Bjorklund, R. A.

    1983-01-01

    A 24-month coupon test program to evaluate the decomposition of propellant tanks is reported. The propellant fuel evaluated was monopropellant-grade hydrazine (N2H4), which is normally a colorless, fuming, corrosive, strongly reducing liquid. The degree of hydrazine decomposition was determined by means of chemical analyses of the liquid and evolved gases at the end of the test program. The experimental rates of hydrazine decomposition were determined to be within acceptable limits. The propellant tank materials and material combinations were not degraded by a 2-year exposure to hydrazine propellant. This was verified using change-of-weight determinations and microscopic examination of the specimen surface before and after exposure, and by posttest chemical analyses of hydrazine liquid for residual metal content.

  18. 20 CFR 404.1599 - Work incentive experiments and rehabilitation demonstration projects in the disability program.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Work incentive experiments and rehabilitation... Continuing Or Stopping Disability § 404.1599 Work incentive experiments and rehabilitation demonstration... Disability Amendments of 1980, Pub. L. 96-265, directs the Commissioner to develop and conduct...

  19. 77 FR 8837 - Termination of the Department of Defense Web-Based TRICARE Assistance Program Demonstration

    Science.gov (United States)

    2012-02-15

    ... family difficulties and pressures, crisis intervention, anxiety, self-esteem, loneliness, and critical... effective August 1, 2009, as referenced in the original Federal Register Notice, 74 FR 36676, July 24, 2009. The demonstration was extended to March 31, 2011, as referenced by Federal Register Notice, 75...

  20. Development and demonstration flight of a fuel cell system for high-altitude balloons

    Energy Technology Data Exchange (ETDEWEB)

    Uno, Masatoshi; Shimada, Takanobu; Sone, Yoshitsugu; Saito, Yoshitaka [Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 3-1-1 Yoshinodai, Sagamihara, Kanagawa 229-8510 (Japan); Ariyama, Yusuke; Fukuzawa, Naoya; Noguchi, Daisuke [Hosei University, Koganei, Tokyo 184-8584 (Japan); Ogawa, Keita [Advanced Engineering Services, Tsukuba, Ibaraki 305-0032 (Japan); Naito, Hitoshi [Aerospace Research and Development Directorate, Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505 (Japan)

    2009-09-05

    Proton exchange membrane fuel cell offers higher energy density than the existing battery technologies for high-energy applications, and it is a promising power source for various industries including aerospace vehicles. We have been developing and testing a non-external humidified fuel cell system for high-altitude balloons, which require simple, light, and easy-to-operate power systems. This system consists of three major subsystems - a fuel cell stack, a reactant supply subsystem, and an electrical control subsystem. Ground performance testing in a vacuum chamber simulating the high-altitude vacuum condition was performed before the flight. Then, a demonstration flight of the fuel cell system was launched using a large balloon for verifying its performance under practical high-altitude conditions. Cell voltage variations synchronized with oxygen pressure spikes were observed that were probably caused by condensed product water plugging the flow passages of the back pressure regulator. Flight results indicated that the fuel cell system operated better when water was expelled as vapor, rather than in the liquid form. In addition, a back pressure regulator should be installed to avoid the accumulation of water droplets for realizing a stable performance. (author)

  1. Zero Emission Bay Area (ZEBA) Fuel Cell Bus Demonstration: Second Results Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Chandler, K.

    2012-07-01

    This report presents results of a demonstration of 12 new fuel cell electric buses (FCEB) operating in Oakland, California. The 12 FCEBs operate as a part of the Zero Emission Bay Area (ZEBA) Demonstration, which also includes two new hydrogen fueling stations. This effort is the largest FCEB demonstration in the United States and involves five participating transit agencies. The ZEBA partners are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory (NREL) to evaluate the buses in revenue service. The first results report was published in August 2011, describing operation of these new FCEBs from September 2010 through May 2011. New results in this report provide an update through April 2012.

  2. Zero Emission Bay Area (ZEBA) Fuel Cell Bus Demonstration Results: Third Report

    Energy Technology Data Exchange (ETDEWEB)

    Eudy, L.; Post, M.

    2014-05-01

    This report presents results of a demonstration of 12 fuel cell electric buses (FCEB) operating in Oakland, California. The 12 FCEBs operate as a part of the Zero Emission Bay Area (ZEBA) Demonstration, which also includes two new hydrogen fueling stations. This effort is the largest FCEB demonstration in the United States and involves five participating transit agencies. The ZEBA partners are collaborating with the U.S. Department of Energy (DOE) and DOE's National Renewable Energy Laboratory (NREL) to evaluate the buses in revenue service. NREL has published two previous reports, in August 2011 and July 2012, describing operation of these buses. New results in this report provide an update covering eight months through October 2013.

  3. Antisense bcl-2 treatment increases programmed cell death in non-small cell lung cancer cell lines.

    Science.gov (United States)

    Koty, P P; Zhang, H; Levitt, M L

    1999-02-01

    Programmed cell death (PCD) is a genetically regulated pathway that is altered in many cancers. This process is, in part, regulated by the ratio of PCD inducers (Bax) or inhibitors (Bcl-2). An abnormally high ratio of Bcl-2 to Bax prevents PCD, thus contributing to resistance to chemotherapeutic agents, many of which are capable of inducing PCD. Non-small cell lung cancer (NSCLC) cells demonstrate resistance to these PCD-inducing agents. If Bcl-2 prevents NSCLC cells from entering the PCD pathway, then reducing the amount of endogenous Bcl-2 product may allow these cells to spontaneously enter the PCD pathway. Our purpose was to determine the effects of bcl-2 antisense treatment on the levels of programmed cell death in NSCLC cells. First, we determined whether bcl-2 and bax mRNA were expressed in three morphologically distinct NSCLC cell lines: NCI-H226 (squamous), NCI-H358 (adenocarcinoma), and NCI-H596 (adenosquamous). Cells were then exposed to synthetic antisense bcl-2 oligonucleotide treatment, after which programmed cell death was determined, as evidenced by DNA fragmentation. Bcl-2 protein expression was detected immunohistochemically. All three NSCLC cell lines expressed both bcl-2 and bax mRNA and had functional PCD pathways. Synthetic antisense bcl-2 oligonucleotide treatment resulted in decreased Bcl-2 levels, reduced cell proliferation, decreased cell viability, and increased levels of spontaneous PCD. This represents the first evidence that decreasing Bcl-2 in three morphologically distinct NSCLC cell lines allows the cells to spontaneously enter a PCD pathway. It also indicates the potential therapeutic use of antisense bcl-2 in the treatment of NSCLC. PMID:10217615

  4. General Electric Company study for defining the number of residential and non-residential projects, National Solar Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    1976-04-01

    The methodology used to perform the ''Parametric Study'' to define a recommended demonstration program involved a decision making process. In this approach, selective solar buying factors were quantitatively evaluated for influencing key decision makers to install solar HVAC equipment. The selection of the recommended demo level also considered the probability of a decision maker actually seeing a demonstration as a function of how far he is located from a demo and how far he is willing to travel to see one. Demonstration levels of 200, 400, 800, 1600 and 3200 were assumed to determine the effects on Solar HVAC market penetration. The 800 demo level program is recommended to effectively stimulate the private sector to install solar HVAC systems. (WDM)

  5. The ISS as a Testbed for Future Large Astronomical Observatories: The OpTIIX Demonstration Program

    Science.gov (United States)

    Burdick, G.; Callen, P.; Ess, K.; Liu, F.; Postman, M.; Sparks, W.; Seery, B.; Thronson, H.

    2012-01-01

    Future large (diameters in excess of approx. 10 m) astronomical observatories in space will need to employ advanced technologies if they are to be affordable. Many of these technologies are ready to be validated on orbit and the International Space Station (ISS) provides a suitable platform for such demonstrations. These technologies include low-cost, low-density, highly deformable mirror segments, coupled with advanced sensing and control methods. In addition, the ISS offers available telerobotic assembly techniques to build an optical testbed that embodies this new cost-effective approach to assemble and achieve diffraction-limited optical performance for very large space telescopes. Given the importance that NASA attaches to the recommendations of the National Academy of Sciences "Decadal Survey" process, essential capabilities and technologies will be demonstrated well in advance of the next Survey, which commences in 2019. To achieve this objective, the Jet Propulsion Laboratory (JPL), NASA Johnson Space Center (JSC), NASA Goddard Space Flight Center (GSFC), and the Space Telescope Science Institute (STScI) are carrying out a Phase A/B study of the Optical Testbed and Integration on ISS eXperiment (OpTIIX). The overarching goal is to demonstrate well before the end of this decade key capabilities intended to enable very large optical systems in the decade of the 2020s. Such a demonstration will retire technical risk in the assembly, alignment, calibration, and operation of future space observatories. The OpTIIX system, as currently designed, is a six-hexagon element, segmented visual-wavelength telescope with an edge-to-edge aperture of 1.4 m, operating at its diffraction limit,

  6. Learning Trajectories for Robot Programing by Demonstration Using a Coordinated Mixture of Factor Analyzers.

    Science.gov (United States)

    Field, Matthew; Stirling, David; Pan, Zengxi; Naghdy, Fazel

    2016-03-01

    This paper presents an approach for learning robust models of humanoid robot trajectories from demonstration. In this formulation, a model of the joint space trajectory is represented as a sequence of motion primitives where a nonlinear dynamical system is learned by constructing a hidden Markov model (HMM) predicting the probability of residing in each motion primitive. With a coordinated mixture of factor analyzers as the emission probability density of the HMM, we are able to synthesize motion from a dynamic system acting along a manifold shared by both demonstrator and robot. This provides significant advantages in model complexity for kinematically redundant robots and can reduce the number of corresponding observations required for further learning. A stability analysis shows that the system is robust to deviations from the expected trajectory as well as transitional motion between manifolds. This approach is demonstrated experimentally by recording human motion with inertial sensors, learning a motion primitive model and correspondence map between the human and robot, and synthesizing motion from the manifold to control a 19 degree-of-freedom humanoid robot. PMID:25826815

  7. Programmed cell death in plants and caspase-like activities

    NARCIS (Netherlands)

    Gaussand, Gwénael Martial Daniel Jean-Marie

    2007-01-01

    The development of multicellular organisms involves an important balance between cell growth, cell division and cell death. In animals, programmed cell death (PCD) plays a key role by forming and deleting structures, controlling cell numbers and eliminating abnormal damaged cells. Caspases were foun

  8. Utility Advanced Turbine Systems Program (ATS) Technical Readiness Testing and Pre-Commercial Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Siemens Westinghouse

    2001-09-30

    The objective of the ATS program is to develop ultra-high efficiency, environmentally superior and cost competitive gas turbine systems for base load application in utility, independent power producer and industrial markets. Specific performance targets have been set using natural gas as the primary fuel: (1) System efficiency that will exceed 60% (lower heating value basis) on natural gas for large scale utility turbine systems; for industrial applications, systems that will result in a 15% improvement in heat rate compared to currently available gas turbine systems. (2) An environmentally superior system that will not require the use of post combustion emissions controls under full load operating conditions. (3) Busbar energy costs that are 10% less than current state-of-the-art turbine systems, while meeting the same environmental requirements. (4) Fuel-flexible designs that will operate on natural gas but are capable of being adapted to operate on coal-derived or biomass fuels. (5) Reliability-Availability-Maintainability (RAM) that is equivalent to the current turbine systems. (6) Water consumption minimized to levels consistent with cost and efficiency goals. (7) Commercial systems that will enter the market in the year 2000. In Phase I of the ATS program, Siemens Westinghouse found that efficiency significantly increases when the traditional combined-cycle power plant is reconfigured with closed-loop steam cooling of the hot gas path. Phase II activities involved the development of a 318MW natural gas fired turbine conceptual design with the flexibility to burn coal-derived and biomass fuels. Phases I and II of the ATS program have been completed. Phase III, the current phase, completes the research and development activities and develops hardware specifications from the Phase II conceptual design. This report summarizes Phase III Extension activities for a three month period. Additional details may be found in monthly technical progress reports covering the

  9. Novel "hybrid" iron chelators derived from aroylhydrazones and thiosemicarbazones demonstrate selective antiproliferative activity against tumor cells.

    Science.gov (United States)

    Lovejoy, David B; Richardson, Des R

    2002-07-15

    We previously demonstrated that 2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone (311) and other aroylhydrazone chelators possess potent antineoplastic activity because of their ability to bind iron (Fe). From these studies, we identified structural components of the hydrazones that provide antineoplastic activity, namely the salicylaldehyde and 2-hydroxy-1-naphthylaldehyde moieties. A related group of chelators known as the thiosemicarbazones also show pronounced antitumor activity because of their ability to inhibit ribonucleotide reductase. Considering this, we designed a new series of "hybrid ligands" by condensation of the aldehydes described above with a range of thiosemicarbazides. The parent compound of these ligands is 2-hydroxy-1-naphthylaldehyde thiosemicarbazone (NT). Of 8 NT analogues, 3 chelators, namely NT, N4mT (2-hydroxy-1-naphthylaldehyde-4-methyl-3-thiosemicarbazone), and N44mT (2-hydroxy-1-naphthylaldehyde-4,4-dimethyl-3-thiosemicarbazone), showed high antiproliferative activity against SK-N-MC neuroepithelioma cells (50% inhibitory concentration [IC(50)] = 0.5-1.5 microM). Indeed, their activity was significantly (P <.0001) greater than that of desferrioxamine (DFO) (IC(50) = 22 microM). We demonstrate that 311, a 311 analogue (311m), and several NT-series chelators have significantly (P <.001) greater antiproliferative activity against tumor cells than against a range of normal cell types. For example, the IC(50) values of NT and N4mT in SK-N-MC neuroepithelioma cells were 0.5 microM, whereas for fibroblasts the IC(50) values were greater than 25 microM. Further, the effect of one of the most potent chelators (311m) on preventing the growth of bone marrow stem cell cultures was far less than that of doxorubicin and similar to that of cisplatin. These studies support the further development of these chelators as antiproliferative agents. PMID:12091363

  10. DEMONSTRATION OF THE GLYCOLIC-FORMIC FLOWSHEET IN THE SRNL SHIELDED CELLS USING ACTUAL WASTE

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, D.; Pareizs, J.; Click, D.

    2011-11-07

    Glycolic acid was effective at dissolving many metals, including iron, during processing with simulants. Criticality constraints take credit for the insolubility of iron during processing to prevent criticality of fissile materials. Testing with actual waste was needed to determine the extent of iron and fissile isotope dissolution during Chemical Process Cell (CPC) processing. The Alternate Reductant Project was initiated by the Savannah River Remediation (SRR) Company to explore options for the replacement of the nitric-formic flowsheet used for the CPC at the Defense Waste Processing Facility (DWPF). The goals of the Alternate Reductant Project are to reduce CPC cycle time, increase mass throughput of the facility, and reduce operational hazards. In order to achieve these goals, several different reductants were considered during initial evaluations conducted by Savannah River National Laboratory (SRNL). After review of the reductants by SRR, SRNL, and Energy Solutions (ES) Vitreous State Laboratory (VSL), two flowsheets were further developed in parallel. The two flowsheet options included a nitric-formic-glycolic flowsheet, and a nitric-formic-sugar flowsheet. As of July 2011, SRNL and ES/VSL have completed the initial flowsheet development work for the nitric-formic-glycolic flowsheet and nitric-formic-sugar flowsheet, respectively. On July 12th and July 13th, SRR conducted a Systems Engineering Evaluation (SEE) to down select the alternate reductant flowsheet. The SEE team selected the Formic-Glycolic Flowsheet for further development. Two risks were identified in SEE for expedited research. The first risk is related to iron and plutonium solubility during the CPC process with respect to criticality. Currently, DWPF credits iron as a poison for the fissile components of the sludge. Due to the high iron solubility observed during the flowsheet demonstrations with simulants, it was necessary to determine if the plutonium in the radioactive sludge slurry

  11. Development of analytical cell support for vitrification at the West Valley Demonstration Project. Topical report

    Energy Technology Data Exchange (ETDEWEB)

    Barber, F.H.; Borek, T.T.; Christopher, J.Z. [and others

    1997-12-01

    Analytical and Process Chemistry (A&PC) support is essential to the high-level waste vitrification campaign at the West Valley Demonstration Project (WVDP). A&PC characterizes the waste, providing information necessary to formulate the recipe for the target radioactive glass product. High-level waste (HLW) samples are prepared and analyzed in the analytical cells (ACs) and Sample Storage Cell (SSC) on the third floor of the main plant. The high levels of radioactivity in the samples require handling them in the shielded cells with remote manipulators. The analytical hot cells and third floor laboratories were refurbished to ensure optimal uninterrupted operation during the vitrification campaign. New and modified instrumentation, tools, sample preparation and analysis techniques, and equipment and training were required for A&PC to support vitrification. Analytical Cell Mockup Units (ACMUs) were designed to facilitate method development, scientist and technician training, and planning for analytical process flow. The ACMUs were fabricated and installed to simulate the analytical cell environment and dimensions. New techniques, equipment, and tools could be evaluated m in the ACMUs without the consequences of generating or handling radioactive waste. Tools were fabricated, handling and disposal of wastes was addressed, and spatial arrangements for equipment were refined. As a result of the work at the ACMUs the remote preparation and analysis methods and the equipment and tools were ready for installation into the ACs and SSC m in July 1995. Before use m in the hot cells, all remote methods had been validated and four to eight technicians were trained on each. Fine tuning of the procedures has been ongoing at the ACs based on input from A&PC technicians. Working at the ACs presents greater challenges than had development at the ACMUs. The ACMU work and further refinements m in the ACs have resulted m in a reduction m in analysis turnaround time (TAT).

  12. Development of analytical cell support for vitrification at the West Valley Demonstration Project. Topical report

    International Nuclear Information System (INIS)

    Analytical and Process Chemistry (A ampersand PC) support is essential to the high-level waste vitrification campaign at the West Valley Demonstration Project (WVDP). A ampersand PC characterizes the waste, providing information necessary to formulate the recipe for the target radioactive glass product. High-level waste (HLW) samples are prepared and analyzed in the analytical cells (ACs) and Sample Storage Cell (SSC) on the third floor of the main plant. The high levels of radioactivity in the samples require handling them in the shielded cells with remote manipulators. The analytical hot cells and third floor laboratories were refurbished to ensure optimal uninterrupted operation during the vitrification campaign. New and modified instrumentation, tools, sample preparation and analysis techniques, and equipment and training were required for A ampersand PC to support vitrification. Analytical Cell Mockup Units (ACMUs) were designed to facilitate method development, scientist and technician training, and planning for analytical process flow. The ACMUs were fabricated and installed to simulate the analytical cell environment and dimensions. New techniques, equipment, and tools could be evaluated m in the ACMUs without the consequences of generating or handling radioactive waste. Tools were fabricated, handling and disposal of wastes was addressed, and spatial arrangements for equipment were refined. As a result of the work at the ACMUs the remote preparation and analysis methods and the equipment and tools were ready for installation into the ACs and SSC m in July 1995. Before use m in the hot cells, all remote methods had been validated and four to eight technicians were trained on each. Fine tuning of the procedures has been ongoing at the ACs based on input from A ampersand PC technicians. Working at the ACs presents greater challenges than had development at the ACMUs. The ACMU work and further refinements m in the ACs have resulted m in a reduction m in

  13. Office of Technology Development Program for Research, Development, Demonstration, Testing, and Evaluation

    International Nuclear Information System (INIS)

    DOE has set a goal to clean up its complex and to bring all sites into compliance with applicable environmental regulations. This initiative is slated for completion by the year 2019. Four years ago there was no coordinated plan for identifying or cleaning these contaminated sites. Since 1989, DOE's Office of Environmental Restoration and Waste Management has invested time, money, and manpower to establish a wide range of programs to meet this immense challenge. DOE is responsible for waste management and clean up of more than 100 contaminated installations in 36 states and territories. This includes 3,700 sites: over 26,000 acres, with hazardous or radioactive contaminated surface or groundwater, soil, or structures; over 26,000 acres requiring remediation, with the number growing as new sites are defined; 500 surplus facilities awaiting decontamination and decommissioning and approximately 5,000 peripheral properties (residences, businesses) that have soil contaminated with uranium tailings

  14. Industrial Fuel Gas Demonstration Plant Program. Bid packages for materials (Deliverable No. 28)

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-01-01

    Fixed-price supply type bid packages for materials and/or service essentially are comprised of two parts, namely: (1) a technical requisition of the material, equipment, or service to be supplied; and (2) commercial and legal requirements, normally referred to as terms and conditions. Requisitions, providing technical requirements, for all equipment items identified for the Industrial Fuel Gas Demonstration Plant may be found in the 12 volumes of the Demonstration Plant Mechanical Design. The requisitions have been included within separate sections of the design report, sorted by appropriate plant unit. Combined with any General Notes Requisition and the necessary FWEC Job Standards, these various item requisitions provide all technical information for the prospective vendor to furnish his bid. The terms and conditions (boiler plate) to be included in the bid package identify all the contractual requirements which will be imposed upon the bidder. These requirements cover the conditions he must meet to bid on the particular item as well as the clauses to be included within the eventual purchase order/subcontract. A typical package of such terms and conditions is included.

  15. Waste management technology development and demonstration programs at Brookhaven National Laboratory

    Science.gov (United States)

    Kalb, Paul D.; Colombo, Peter

    1991-01-01

    Two thermoplastic processes for improved treatment of radioactive, hazardous, and mixed wastes were developed from bench scale through technology demonstration: polyethylene encapsulation and modified sulfur cement encapsulation. The steps required to bring technologies from the research and development stage through full scale implementation are described. Both systems result in durable waste forms that meet current Nuclear Regulatory Commission and Environmental Protection Agency regulatory criteria and provide significant improvements over conventional solidification systems such as hydraulic cement. For example, the polyethylene process can encapsulate up to 70 wt pct. nitrate salt, compared with a maximum of about 20 wt pct. for the best hydraulic cement formulation. Modified sulfur cement waste forms containing as much as 43 wt pct. incinerator fly ash were formulated, whereas the maximum quantity of this waste in hydraulic cement is 16 wt pct.

  16. Industrial fuel gas demonstration plant program. Construction permit. Compliance plan. (Deliverable No. 31)

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    The objective of this compliance plan is to insure that all required permits are filed and obtained prior to the start of construction of the U-gas demonstration plant. This plan addresses the permits in the following areas: construction, electrical, mechanical, plumbing, federal aviation lighting, and as-required permits. Each permit area is introduced by a brief summary of the permits required and the significant circumstances and/or conditions affecting permit acquisition. Each permit is then discussed in detail according to a format which includes the following: brief introduction of permit, responsible regulator agency, other potential reviewing agency(s), information needed for permit, filing procedures, normal review period, permit duration period, and permit fees. Copies of the actual application forms, guidelines for completing the applications, statements on required information and agency contacts are contained in the Appendices.

  17. EPA program to demonstrate mitigation measures for indoor radon: initial results

    International Nuclear Information System (INIS)

    EPA has installed radon mitigation techniques in 18 concrete block basement homes in the Reading Prong region of eastern Pennsylvania. Three alternative active soil ventilation approaches were tested: suction on the void network within the concrete block basement walls; suction on the footing drain tile system; and suction on the aggregate underneath the concrete slab. The initial 18 mitigation installations were designed to demonstrate techniques which would have low to moderate installation and operating costs. Where effective closure of major openings in the block walls is possible, suction on the wall voids has proved to be extremely effective, able to reduce homes having very high radon Working Levels (up to 7 WL) to 0.02 WL and less. However, where inaccessible major openings are concealed within the wall, it is more difficult and/or more expensive to develop adequate suction on the void network, and performance is reduced. Testing is continuing to demonstrate the steps required to achieve high performance with wall suction in homes with such difficult-to close walls. Drain tile suction can be very effective where the drain tiles completely surround the home; drain tile suction is the least expensive and most aesthetic of the active soil ventilation approaches, but appears susceptible to spikes in radon levels when the basement is depressurized. Sub-slab suction as tested in this study - with one or two individual suction points in the slab - does not appear adequate to ensure sustained high levels of reduction on block wall basement homes; it appears to effectively treat slab-related soil gas entry routes so long as a uniform layer of aggregate is present, but it does not appear to effectively treat the wall-related entry routes. Closure of major openings might have improved sub-slab suction performance. 5 figures, 3 tables

  18. Identification of sites for the low-level waste disposal development and demonstration program

    International Nuclear Information System (INIS)

    This report presents the results of site selection studies for potential low-level radioactive waste disposal sites on the Oak Ridge Reservation (ORR). Summaries of the site selection procedures used and results of previous site selection studies on the ORR are included. This report includes recommendations of sites for demonstration of shallow land burial using engineered trench designs and demonstration of above-grade disposal using design concepts similar to those used in tumulus disposal. The site selection study, like its predecessor (ORNL/TM-9717, Use of DOE Site Selection Criteria for Screening Low-Level Waste Disposal Sites on the Oak Ridge Reservation), involved application of exclusionary site screening criteria to the region of interest to eliminate unacceptable areas from consideration. Also like the previous study, the region of interest for this study was limited to the Oak Ridge Department of Energy Reservation. Reconnaissance-level environmental data were used in the study, and field inspections of candidate sites were made to verify the available reconnaissance data. Five candidate sites, all underlain by Knox dolomite residuum and bedrock, were identified for possible development of shallow land burial facilities. Of the five candidate sites, the West Chestnut site was judged to be best suited for deployment of the shallow land burial technology. Three candidate sites, all underlain by the Conasauga Group in Bear Creek Valley, were identified for possible development of above-grade disposal technologies. Of the three sites identified, the Central Bear Creek Valley site lying between State Route 95 and Gum Hollow Road was ranked most favorable for deployment of the above-grade disposal technology

  19. Acetylsalicylic acid induces programmed cell death in Arabidopsis cell cultures.

    Science.gov (United States)

    García-Heredia, José M; Hervás, Manuel; De la Rosa, Miguel A; Navarro, José A

    2008-06-01

    Acetylsalicylic acid (ASA), a derivative from the plant hormone salicylic acid (SA), is a commonly used drug that has a dual role in animal organisms as an anti-inflammatory and anticancer agent. It acts as an inhibitor of cyclooxygenases (COXs), which catalyze prostaglandins production. It is known that ASA serves as an apoptotic agent on cancer cells through the inhibition of the COX-2 enzyme. Here, we provide evidences that ASA also behaves as an agent inducing programmed cell death (PCD) in cell cultures of the model plant Arabidopsis thaliana, in a similar way than the well-established PCD-inducing agent H(2)O(2), although the induction of PCD by ASA requires much lower inducer concentrations. Moreover, ASA is herein shown to be a more efficient PCD-inducing agent than salicylic acid. ASA treatment of Arabidopsis cells induces typical PCD-linked morphological and biochemical changes, namely cell shrinkage, nuclear DNA degradation, loss of mitochondrial membrane potential, cytochrome c release from mitochondria and induction of caspase-like activity. However, the ASA effect can be partially reverted by jasmonic acid. Taking together, these results reveal the existence of common features in ASA-induced animal apoptosis and plant PCD, and also suggest that there are similarities between the pathways of synthesis and function of prostanoid-like lipid mediators in animal and plant organisms.

  20. Binary Optical True-Time Delay Based on the White Cell: Design and Demonstration

    Science.gov (United States)

    Anderson, Betty Lise; Rabb, David J.; Warnky, Carolyn M.; Abou-Galala, Feras

    2006-04-01

    An optical true-time delay device that uses a binary counting system in a modified White cell is demonstrated. The switching engine uses four spherical mirrors and a three-state digital micromirror array. The delay part, as designed, provides 6 bits of delay ranging from 78 ps to 5 ns, using a combination of dielectric blocks for short delays and lens trains for longer ones. Long lens trains are folded for compactness. The authors describe the design and demonstrate two of the 6 bits of delays experimentally. Delays were accurate to within the measurement resolution of 1.25 ps. The insertion loss varied from 3.1-5.2 dB, depending on delay. It was found that the micromirrors do not contribute significantly to the loss.

  1. Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans.

    Science.gov (United States)

    Tang, Huangqi; Chen, Liangwen; Liu, Jialu; Shi, Jue; Li, Qingqing; Wang, Ting; Wu, Lijun; Zhan, Furu; Bian, Po

    2016-04-01

    Reproductive cell death (RCD) occurs after one or more cell divisions resulting from an insult such as radiation exposure or other treatments with carcinogens or mutagens. The radioadaptive response for RCD is usually investigated by in vitro or in vivo clonogenic assay. To date, this has not been demonstrated in the vulval tissue in Caenorhabditis elegans ( C. elegans ), which is a well established in vivo model for radiation-induced RCD. In this study to determine whether radioadaptive response occurs in the vulval tissue model of C. elegans , early larval worms were gamma irradiated with lower adaptive doses, followed by higher challenge doses. The ratio of protruding vulva was used to assess the RCD of vulval cells. The results of this study showed that the radioadaptive response for RCD in this vulval tissue model could be well induced by dose combinations of 5 + 75 Gy and 5 + 100 Gy at the time point of 14-16 h in worm development. In addition, the time course analysis indicated that radioresistance in vulval cells developed within 1.75 h after an adaptive dose and persisted for only a short period of time (2-4 h). DNA damage checkpoint and non-homologous end joining were involved in the radioadaptive response, exhibiting induction of protruding vulva in worms deficient in these two pathways similar to their controls. Interestingly, the DNA damage checkpoint was not active in the somatic vulval cells, and it was therefore suggested that the DNA damage checkpoint might mediate the radioadaptive response in a cell nonautonomous manner. Here, we show evidence of the occurrence of a radioadaptive response for RCD in the vulval tissue model of C. elegans . This finding provides a potential opportunity to gain further insight into the underlying mechanisms of the radioadaptive response for RCD, in view of the abundant genetic resources of C. elegans . PMID:27023260

  2. A1 demonstrates restricted tissue distribution during embryonic development and functions to protect against cell death.

    Science.gov (United States)

    Carrió, R.; López-Hoyos, M.; Jimeno, J.; Benedict, M. A.; Merino, R.; Benito, A.; Fernández-Luna, J. L.; Núñez, G.; García-Porrero, J. A.; Merino, J.

    1996-01-01

    Members of the bcl-2 gene family are essential regulators of cell survival in a wide range of biological processes. A1, a member of the family, is known to be expressed in certain adult tissues. However, the precise tissue distribution and function of A1 remains poorly understood. We show here that A1 is expressed in multiple tissues during murine embryonic development. In the embryo, A1 was detected first at embryonic day 11.5 in liver, brain, and limbs. At day 13.5 of gestation, A1 expression was observed in the central nervous system, liver, perichondrium, and digital zones of developing limbs in a pattern different from that of bcl-X. In the central nervous system of 15.5-day embryos, A1 was expressed at high levels in the ventricular zone and cortical plate of brain cortex. Significantly, the interdigital zones of limbs and the intermediate region of the developing brain cortex, two sites associated with extensive cell death, were devoid of A1 and bcl-X. The expression of A1 was retained in many adult tissues. To assess the ability of A1 to modulate cell death, stable transfectants expressing different amounts of A1 protein were generated in K562 cells. Expression of A1 was associated with retardation of apoptotic cell death induced by actinomycin D and cycloheximide as well as by okadaic acid. Confocal microscopy showed that the A1 protein was localized to the cytoplasm in a pattern similar to that of Bcl-2. These results demonstrate that the expression of A1 is wider than previously reported in adult tissues. Furthermore, its distribution in multiple tissues of the embryo suggests that A1 plays a role in the regulation of physiological cell death during embryonic development. Images Figure 1 Figure 2 Figure 3 Figure 5 PMID:8952545

  3. DC-9 flight demonstration program with refanned JT8D engines. Volume 1: Summary

    Science.gov (United States)

    1975-01-01

    The design, analysis, fabrication, and ground and flight testing of DC-9 airframe/nacelle hardware with prototype JT8D-109 engines are discussed. The installation of the JT8D-109 engine on the DC-9 Refan airplane required new or modified hardware for the pylon, nacelle, and fuselage. The acoustic material used in the nose cowl was bonded aluminum honeycomb sandwich and the exhaust duct acoustic material was Inconel 625 Stresskin. The sea level static, standard day bare engine takeoff thrust, the cruise TSFC and the maximum available cruise thrust for the JT8D-109 engine were compared with those of the JT8D-9 engine. The range capabilities of the DC-9 Refan and the production DC-9 airplane were also compared. The Refan airplane demonstrated flight characteristics similar to the production DC-9-30 and satisfied airworthiness requirements. Flyover noise levels were determined for the DC-9 Refan and the DC-9 C-9A airplane for takeoff and landing conditions. Cost estimates were also made.

  4. DARPA counter-sniper program: Phase 1 Acoustic Systems Demonstration results

    Science.gov (United States)

    Carapezza, Edward M.; Law, David B.; Csanadi, Christina J.

    1997-02-01

    During October 1995 through May 1996, the Defense Advanced Research Projects Agency sponsored the development of prototype systems that exploit acoustic muzzle blast and ballistic shock wave signatures to accurately predict the location of gunfire events and associated shooter locations using either single or multiple volumetric arrays. The output of these acoustic systems is an estimate of the shooter location and a classification estimate of the caliber of the shooter's weapon. A portable display and control unit provides both graphical and alphanumeric shooter location related information integrated on a two- dimensional digital map of the defended area. The final Phase I Acoustic Systems Demonstration field tests were completed in May. These these tests were held at USMC Base Camp Pendleton Military Operations Urban Training (MOUT) facility. These tests were structured to provide challenging gunfire related scenarios with significant reverberation and multi-path conditions. Special shot geometries and false alarms were included in these tests to probe potential system vulnerabilities and to determine the performance and robustness of the systems. Five prototypes developed by U.S. companies and one Israeli developed prototype were tested. This analysis quantifies the spatial resolution estimation capability (azimuth, elevation and range) of these prototypes and describes their ability to accurately classify the type of bullet fired in a challenging urban- like setting.

  5. Testing Educational Tools to Demonstrate Returns to Work for Children Aging Out of the SSI-Disabled Children Program

    OpenAIRE

    Richard V. Burkhauser; Daly, Mary C.

    2011-01-01

    A substantial fraction of children receiving Supplemental Security Income benefits for disability (SSI-child) transition directly onto the SSI-adult program at age 18 without attempting to enter the labor market. Once this transition is complete, very few attempt to work while receiving SSI-adult benefits. The MDRC SSA Youth Transition Demonstration (YTD) Project report (2008) identified lack of information about financial incentives/disincentives as a potential barrier to work for these yout...

  6. Physical studies of transmutation scenarios. The Muse program with the Masurca facility: a step towards an hybrid demonstrator?

    International Nuclear Information System (INIS)

    The Muse research program, which started in 1995, is a contribution to the development of a dedicated subcritical accelerator driven system (ADS) for the transmutation of minor actinides produced by conventional nuclear power plants. The Muse experiments aim at making a parametric study of different reactor core compositions with different subcritical levels and supplied by different sources in order to demonstrate that the measurement techniques and the calculation charts established for critical FBRs remain valid with an hybrid system. The 4. phase of the Muse program concerns the design, realization and installation of the Genepi (generator of intense pulse neutrons) deutons accelerator at the Masurca facility of Cadarache (France) for the understanding of the neutronic behaviour of an ADS, the definition of a reference calculation scheme, and the development of specific experimental techniques for dynamical measurements. This document brings together the presentations (transparencies) given at the SFEN technical meeting of May 30, 2002 about the Muse program. (J.S.)

  7. Senescence and programmed cell death : substance or semantics?

    NARCIS (Netherlands)

    Doorn, van W.G.; Woltering, E.J.

    2004-01-01

    The terms senescence and programmed cell death (PCD) have led to some confusion. Senescence as visibly observed in, for example, leaf yellowing and petal wilting, has often been taken to be synonymous with the programmed death of the constituent cells. PCD also obviously refers to cells, which show

  8. TRUEX hot demonstration

    International Nuclear Information System (INIS)

    In FY 1987, a program was initiated to demonstrate technology for recovering transuranic (TRU) elements from defense wastes. This hot demonstration was to be carried out with solution from the dissolution of irradiated fuels. This recovery would be accomplished with both PUREX and TRUEX solvent extraction processes. Work planned for this program included preparation of a shielded-cell facility for the receipt and storage of spent fuel from commercial power reactors, dissolution of this fuel, operation of a PUREX process to produce specific feeds for the TRUEX process, operation of a TRUEX process to remove residual actinide elements from PUREX process raffinates, and processing and disposal of waste and product streams. This report documents the work completed in planning and starting up this program. It is meant to serve as a guide for anyone planning similar demonstrations of TRUEX or other solvent extraction processing in a shielded-cell facility

  9. The U.S. Department of Energy`s integrated gasification combined cycle research, development and demonstration program

    Energy Technology Data Exchange (ETDEWEB)

    Brdar, R.D.; Cicero, D.C.

    1996-07-01

    Historically, coal has played a major role as a fuel source for power generation both domestically and abroad. Despite increasingly stringent environmental constraints and affordable natural gas, coal will remain one of the primary fuels for producing electricity. This is due to its abundance throughout the world, low price, ease of transport an export, decreasing capital cost for coal-based systems, and the need to maintain fuel diversity. Recognizing the role coal will continue to play, the US Department of Energy (DOE) is working in partnership with industry to develop ways to use this abundant fuel resource in a manner that is more economical, more efficient and environmentally superior to conventional means to burn coal. The most promising of these technologies is integrated gasification combined cycle (IGCC) systems. Although IGCC systems offer many advantages, there are still several hurdles that must be overcome before the technology achieves widespread commercial acceptance. The major hurdles to commercialization include reducing capital and operating costs, reducing technical risk, demonstrating environmental and technical performance at commercial scale, and demonstrating system reliability and operability. Overcoming these hurdles, as well as continued progress in improving system efficiency, are the goals of the DOE IGCC research, development and demonstrate (RD and D) program. This paper provides an overview of this integrated RD and D program and describes fundamental areas of technology development, key research projects and their related demonstration scale activities.

  10. Advanced industrial gas turbine technology readiness demonstration program. Phase II. Final report: compressor rig fabrication assembly and test

    Energy Technology Data Exchange (ETDEWEB)

    Schweitzer, J. K.; Smith, J. D.

    1981-03-01

    The results of a component technology demonstration program to fabricate, assemble and test an advanced axial/centrifugal compressor are presented. This work was conducted to demonstrate the utilization of advanced aircraft gas turbine cooling and high pressure compressor technology to improve the performance and reliability of future industrial gas turbines. Specific objectives of the compressor component testing were to demonstrate 18:1 pressure ratio on a single spool at 90% polytropic efficiency with 80% fewer airfoils as compared to current industrial gas turbine compressors. The compressor design configuration utilizes low aspect ratio/highly-loaded axial compressor blading combined with a centrifugal backend stage to achieve the 18:1 design pressure ratio in only 7 stages and 281 axial compressor airfoils. Initial testing of the compressor test rig was conducted with a vaneless centrifugal stage diffuser to allow documentation of the axial compressor performance. Peak design speed axial compressor performance demonstrated was 91.8% polytropic efficiency at 6.5:1 pressure ratio. Subsequent documentation of the combined axial/centrifugal performance with a centrifugal stage pipe diffuser resulted in the demonstration of 91.5% polytropic efficiency and 14% stall margin at the 18:1 overall compressor design pressure ratio. The demonstrated performance not only exceeded the contract performance goals, but also represents the highest known demonstrated compressor performance in this pressure ratio and flow class. The performance demonstrated is particularly significant in that it was accomplished at airfoil loading levels approximately 15% higher than that of current production engine compressor designs. The test results provide conclusive verification of the advanced low aspect ratio axial compressor and centrifugal stage technologies utilized.

  11. "Dedicated To The Continued Education, Training and Demonstration of PEM Fuel Cell Powered Lift Trucks In Real-World Applications."

    Energy Technology Data Exchange (ETDEWEB)

    Dever, Thomas J.

    2011-11-29

    The project objective was to further assist in the commercialization of fuel cell and H2 technology by building further upon the successful fuel cell lift truck deployments that were executed by LiftOne in 2007, with longer deployments of this technology in real-world applications. We involved facilities management, operators, maintenance personnel, safety groups, and Authorities Having Jurisdiction. LiftOne strived to educate a broad group from many areas of industry and the community as to the benefits of this technology. Included were First Responders from the local areas. We conducted month long deployments with end-users to validate the value proposition and the market requirements for fuel cell powered lift trucks. Management, lift truck operators, Authorities Having Jurisdiction and the general public experienced 'hands on' fuel cell experience in the material handling applications. We partnered with Hydrogenics in the execution of the deployment segment of the program. Air Products supplied the compressed H2 gas and the mobile fueler. Data from the Fuel Cell Power Packs and the mobile fueler was sent to the DOE and NREL as required. Also, LiftOne conducted the H2 Education Seminars on a rotating basis at their locations for lift trucks users and for other selected segments of the community over the project's 36 month duration. Executive Summary The technology employed during the deployments program was not new, as the equipment had been used in several previous demos and early adoptions within the material handling industry. This was the case with the new HyPx Series PEM - Fuel Cell Power Packs used, which had been demo'd before during the 2007 Greater Columbia Fuel Cell Challenge. The Air Products HF-150 Fueler was used outdoors during the deployments and had similarly been used for many previous demo programs. The methods used centered on providing this technology as the power for electric sit-down lift trucks at high profile companies

  12. Demonstrating hydrogen production from ammonia using lithium imide - Powering a small proton exchange membrane fuel cell

    Science.gov (United States)

    Hunter, Hazel M. A.; Makepeace, Joshua W.; Wood, Thomas J.; Mylius, O. Simon; Kibble, Mark G.; Nutter, Jamie B.; Jones, Martin O.; David, William I. F.

    2016-10-01

    Accessing the intrinsic hydrogen content within ammonia, NH3, has the potential to play a very significant role in the future of a CO2-free sustainable energy supply. Inexpensive light metal imides and amides are effective at decomposing ammonia to hydrogen and nitrogen (2NH3 → 3H2 + N2), at modest temperatures, and thus represent a low-cost approach to on-demand hydrogen production. Building upon this discovery, this paper describes the integration of an ammonia cracking unit with a post-reactor gas purification system and a small-scale PEM fuel cell to create a first bench-top demonstrator for the production of hydrogen using light metal imides.

  13. Assessment of Carbon Tetrachloride Groundwater Transport in Support of the Hanford Carbon Tetrachloride Innovative Technology Demonstration Program

    Energy Technology Data Exchange (ETDEWEB)

    Truex, Michael J.; Murray, Christopher J.; Cole, Charles R.; Cameron, Richard J.; Johnson, Michael D.; Skeen, Rodney S.; Johnson, Christian D.

    2001-07-13

    Groundwater modeling was performed in support of the Hanford Carbon Tetrachloride Innovative Treatment Remediation Demonstration (ITRD) Program. The ITRD program is facilitated by Sandia National Laboratory for the Department of Energy Office of Science and Technology. This report was prepared to document the results of the modeling effort and facilitate discussion of characterization and remediation options for the carbon tetrachloride plume among the ITRD participants. As a first step toward implementation of innovative technologies for remediation of the carbon tetrachloride (CT) plume underlying the 200-West Area, this modeling was performed to provide an indication of the potential impact of the CT source on the compliance boundary approximately 5000 m distant. The primary results of the modeling bracket the amount of CT source that will most likely result in compliance/non-compliance at the boundary and the relative influence of the various modeling parameters.

  14. CELLFS: TAKING THE "DMA" OUT OF CELL PROGRAMMING

    Energy Technology Data Exchange (ETDEWEB)

    IONKOV, LATCHESAR A. [Los Alamos National Laboratory; MIRTCHOVSKI, ANDREY A. [Los Alamos National Laboratory; NYRHINEN, AKI M. [Los Alamos National Laboratory

    2007-01-09

    In this paper we present a new programming model for the Cell BE architecture of scalar multiprocessors. They call this programming model CellFS. CellFS aims at simplifying the task of managing I/O between the local store of the processing units and main memory. The CellFS support library provides the means for transferring data via simple file I/O operations between the PPU and the SPU.

  15. Programmed cell death in plants: A chloroplastic connection

    OpenAIRE

    Ambastha, Vivek; Tripathy, Baishnab C; Tiwari, Budhi Sagar

    2015-01-01

    Programmed cell death (PCD) is an integral cellular program by which targeted cells culminate to demise under certain developmental and pathological conditions. It is essential for controlling cell number, removing unwanted diseased or damaged cells and maintaining the cellular homeostasis. The details of PCD process has been very well elucidated and characterized in animals but similar understanding of the process in plants has not been achieved rather the field is still in its infancy that ...

  16. Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells.

    Science.gov (United States)

    Appelgren, Henrik; Kniola, Barbara; Ekwall, Karl

    2003-10-01

    Fission yeast (Saccharomyces pombe) centromere DNA is organized in a central core region flanked on either side by a region of outer repeat (otr) sequences. The otr region is known to be heterochromatic and bound by the Swi6 protein whereas the central core region contains an unusual chromatin structure involving the histone H3 variant Cnp1 (S. pombe CENP-A). The central core is the base for formation of the kinetochore structure whereas the flanking region is important for sister centromere cohesion. We have previously shown that the ultrastructural domain structure of S. pombe centromeres in interphase is similar to that of human centromeres. Here we demonstrate that S. pombe centromeres are organized in cytologically distinct domains even in mitosis. Fluorescence in situ hybridization of fixed metaphase cells revealed that the otr regions of the centromere were still held together by cohesion even after the sister kinetochores had separated. In live cells, the central cores and kinetochores of sister chromosomes could be distinguished from one another when they were subjected to mitotic tension. The function of the different centromeric domains was addressed. Transacting mutations affecting the kinetochore (nuf2) central core domain (mis6) and the heterochromatin domain (rik1) were analyzed in live cells. In interphase, both nuf2 and mis6 caused declustering of centromeres from the spindle pole body whereas centromere clustering was normal in rik1 despite an apparent decondensation defect. The declustering of centromeres in mis6 cells correlated with loss the Ndc80 kinetochore marker protein from the centromeres. Interestingly the declustered centromeres were still restricted to the nuclear periphery thus revealing a kinetochore-independent peripheral localization mechanism for heterochromatin. Time-lapse microscopy of live mis6 and nuf2-1 mutant cells in mitosis showed similar severe misaggregation phenotypes whereas the rik1 mutants showed a mild cohesion

  17. Environment, safety and health, management and organization compliance assessment, West Valley Demonstration Program, West Valley, New York

    International Nuclear Information System (INIS)

    An Environment, Safety and Health ''Tiger Team'' Assessment was conducted at the West Valley Demonstration Project. The Tiger Team was chartered to conduct an onsite, independent assessment of WVDP's environment, safety and health (ES ampersand H) programs to assure compliance with applicable Federal and State laws, regulations, and standards, and Department of Energy Orders. The objective is to provide to the Secretary of Energy the following information: current ES ampersand H compliance status of each facility; specific noncompliance items; ''root causes'' for noncompliance items; evaluation of the adequacy of ES ampersand H organization and resources (DOE and contractor) and needed modifications; and where warranted, recommendations for addressing identified problem areas

  18. Plant programmed cell death, ethylene and flower senescence

    NARCIS (Netherlands)

    Woltering, E.J.; Jong, de A.; Hoeberichts, F.A.; Iakimova, E.T.; Kapchina, V.

    2005-01-01

    Programmed cell death (PCD) applies to cell death that is part of the normal life of multicellular organisms. PCD is found throughout the animal and plant kingdoms; it is an active process in which a cell suicide pathway is activated resulting in controlled disassembly of the cell. Most cases of PCD

  19. Programmed Cell Death and Postharvest Deterioration of Horticultural Produce

    NARCIS (Netherlands)

    Woltering, E.J.; Iakimova, E.T.

    2010-01-01

    Programmed cell death (PCD) is a process where cells or tissues are broken down in an orderly and predictable manner, whereby nutrients are re-used by other cells, tissues or plant parts. The process of (petal) senescence shows many similarities to autophagic PCD in animal cells including a massive

  20. Programmed Cell-to-Cell Variability in Ras Activity Triggers Emergent Behaviors during Mammary Epithelial Morphogenesis

    Directory of Open Access Journals (Sweden)

    Jennifer S. Liu

    2012-11-01

    Full Text Available Variability in signaling pathway activation between neighboring epithelial cells can arise from local differences in the microenvironment, noisy gene expression, or acquired genetic changes. To investigate the consequences of this cell-to-cell variability in signaling pathway activation on coordinated multicellular processes such as morphogenesis, we use DNA-programmed assembly to construct three-dimensional MCF10A microtissues that are mosaic for low-level expression of activated H-Ras. We find two emergent behaviors in mosaic microtissues: cells with activated H-Ras are basally extruded or lead motile multicellular protrusions that direct the collective motility of their wild-type neighbors. Remarkably, these behaviors are not observed in homogeneous microtissues in which all cells express the activated Ras protein, indicating that heterogeneity in Ras activity, rather than the total amount of Ras activity, is critical for these processes. Our results directly demonstrate that cell-to-cell variability in pathway activation within local populations of epithelial cells can drive emergent behaviors during epithelial morphogenesis.

  1. A school-based human papillomavirus vaccination program in barretos, Brazil: final results of a demonstrative study.

    Directory of Open Access Journals (Sweden)

    José Humberto Tavares Guerreiro Fregnani

    Full Text Available INTRODUCTION: The implementation of a public HPV vaccination program in several developing countries, especially in Latin America, is a great challenge for health care specialists. AIM: To evaluate the uptake and the three-dose completion rates of a school-based HPV vaccination program in Barretos (Brazil. METHODS: THE STUDY INCLUDED GIRLS WHO WERE ENROLLED IN PUBLIC AND PRIVATE SCHOOLS AND WHO REGULARLY ATTENDED THE SIXTH AND SEVENTH GRADES OF ELEMENTARY SCHOOL (MEAN AGE: 11.9 years. A meeting with the parents or guardians occurred approximately one week before the vaccination in order to explain the project and clarify the doubts. The quadrivalent vaccine was administered using the same schedule as in the product package (0-2-6 months. The school visits for regular vaccination occurred on previously scheduled dates. The vaccine was also made available at Barretos Cancer Hospital for the girls who could not be vaccinated on the day when the team visited the school. RESULTS: Among the potential candidates for vaccination (n = 1,574, the parents or guardians of 1,513 girls (96.1% responded to the invitation to participate in the study. A total of 1,389 parents or guardians agreed to participate in the program (acceptance rate = 91.8%. The main reason for refusing to participate in the vaccination program was fear of adverse events. The vaccine uptake rates for the first, second, and third doses were 87.5%, 86.3% and 85.0%, respectively. The three-dose completion rate was 97.2%. CONCLUSIONS: This demonstrative study achieved high rates of vaccination uptake and completion of three vaccine doses in children 10-16 years old from Brazil. The feasibility and success of an HPV vaccination program for adolescents in a developing country may depend on the integration between the public health and schooling systems.

  2. 2015 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-12-23

    The 2015 Annual Progress Report summarizes fiscal year 2015 activities and accomplishments by projects funded by the DOE Hydrogen and Fuel Cells Program. It covers the program areas of hydrogen production; hydrogen delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes and standards; systems analysis; and market transformation.

  3. Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death

    Institute of Scientific and Technical Information of China (English)

    Shan Wang; Zhen Guo; Peng Xia; Tingting Liu; Jufang Wang; Shan Li; Lihua Sun; Jianxin Lu; Qian Wen; Mingqian Zhou; Li Ma; Xia Ding; Xiaoning Wang; Xuebiao Yao

    2009-01-01

    Cytotoxic lymphocytes are key players in the orchestration of immune response and elimination of defective cells. We have previously reported that natural killer (NK) cells enter target tumor cells, leading to either target cell death or self-destruction within tumor cells. However, it has remained elusive as to the fate of NK cells after internaliza-tion and whether the heterotypic cell-in-cell process is different from that of the homotypic cell-in-cell event recently named entosis. Here, we show that NK cells undergo a cell-in-cell process with the ultimate fate of apoptosis within tumor cells and reveal that the internalization process requires the actin cytoskeletal regulator, ezrin. To visualize how NK cells enter into tumor cells, we carried out real-time dual color imaging analyses of NK cell internalization into tumor cells. Surprisingly, most NK cells commit to programmed cell death after their entry into tumor cells, which is distinctively different from entosis observed in the homotypic cell-in-cell process. The apoptotic cell death of the internalized NK cells was evident by activation of caspase 3 and DNA fragmentation. Furthermore, NK cell death after internalization is attenuated by the caspase inhibitor, Z-VAD-FMK, confirming apoptosis as the mode of NK cell death within tumor cells. To determine protein factors essential for the entry of NK cells into tumor cells, we car-ried out siRNA-based knockdown analysis and discovered a critical role of ezrin in NK cell internalization. Impor-tantly, PKA-mediated phosphorylation of ezrin promotes the NK cell internalization process. Our findings suggest a novel regulatory mechanism by which ezrin governs NK cell internalization into tumor cells.

  4. 1990 fuel cell seminar: Program and abstracts

    Energy Technology Data Exchange (ETDEWEB)

    1990-12-31

    This volume contains author prepared short resumes of the presentations at the 1990 Fuel Cell Seminar held November 25-28, 1990 in Phoenix, Arizona. Contained herein are 134 short descriptions organized into topic areas entitled An Environmental Overview, Transportation Applications, Technology Advancements for Molten Carbonate Fuel Cells, Technology Advancements for Solid Fuel Cells, Component Technologies and Systems Analysis, Stationary Power Applications, Marine and Space Applications, Technology Advancements for Acid Type Fuel Cells, and Technology Advancement for Solid Oxide Fuel Cells.

  5. Dynamic Memory Cells Using MoS2 Field-Effect Transistors Demonstrating Femtoampere Leakage Currents.

    Science.gov (United States)

    Kshirsagar, Chaitanya U; Xu, Weichao; Su, Yang; Robbins, Matthew C; Kim, Chris H; Koester, Steven J

    2016-09-27

    Two-dimensional semiconductors such as transition-metal dichalcogenides (TMDs) are of tremendous interest for scaled logic and memory applications. One of the most promising TMDs for scaled transistors is molybdenum disulfide (MoS2), and several recent reports have shown excellent performance and scalability for MoS2 MOSFETs. An often overlooked feature of MoS2 is that its wide band gap (1.8 eV in monolayer) and high effective masses should lead to extremely low off-state leakage currents. These features could be extremely important for dynamic memory applications where the refresh rate is the primary factor affecting the power consumption. Theoretical predictions suggest that leakage currents in the 10(-18) to 10(-15) A/μm range could be possible, even in scaled transistor geometries. Here, we demonstrate the operation of one- and two-transistor dynamic memory circuits using MoS2 MOSFETs. We characterize the retention times in these circuits and show that the two-transistor memory cell reveals MoS2 MOSFETs leakage currents as low as 1.7 × 10(-15) A/μm, a value that is below the noise floor of conventional DC measurements. These results have important implications for the future use of MoS2 MOSFETs in low-power circuit applications.

  6. Adipose-Derived Mesenchymal Stem Cells from Ventral Hernia Repair Patients Demonstrate Decreased Vasculogenesis

    Directory of Open Access Journals (Sweden)

    Jeffrey Lisiecki

    2014-01-01

    Full Text Available Introduction. In adipose tissue healing, angiogenesis is stimulated by adipose-derived stromal stem cells (ASCs. Ventral hernia repair (VHR patients are at high risk for wound infections. We hypothesize that ASCs from VHR patients are less vasculogenic than ASCs from healthy controls. Methods. ASCs were harvested from the subcutaneous fat of patients undergoing VHR by the component separation technique and from matched abdominoplasty patients. RNA and protein were harvested on culture days 0 and 3. Both groups of ASCs were subjected to hypoxic conditions for 12 and 24 hours. RNA was analyzed using qRT-PCR, and protein was used for western blotting. ASCs were also grown in Matrigel under hypoxic conditions and assayed for tubule formation after 24 hours. Results. Hernia patient ASCs demonstrated decreased levels of VEGF-A protein and vasculogenic RNA at 3 days of growth in differentiation media. There were also decreases in VEGF-A protein and vasculogenic RNA after growth in hypoxic conditions compared to control ASCs. After 24 hours in hypoxia, VHR ASCs formed fewer tubules in Matrigel than in control patient ASCs. Conclusion. ASCs derived from VHR patients appear to express fewer vasculogenic markers and form fewer tubules in Matrigel than ASCs from abdominoplasty patients, suggesting decreased vasculogenic activity.

  7. Bachelor of Science-Engineering Technology Program and Fuel Cell Education Program Concentration

    Energy Technology Data Exchange (ETDEWEB)

    Block, David L. [Florida Solar Energy Center, Cocoa, FL (United States); Sleiti, Ahmad [Univ. of North Carolina, Charlotte, NC (United States)

    2011-09-19

    The Hydrogen and Fuel Cell Technology education project has addressed DOE goals by supplying readily available, objective, technical, and accurate information that is available to students, industry and the public. In addition, the program has supplied educated trainers and training opportunities for the next generation workforce needed for research, development, and demonstration activities in government, industry, and academia. The project has successfully developed courses and associated laboratories, taught the new courses and labs and integrated the HFCT option into the accredited engineering technology and mechanical engineering programs at the University of North Carolina at Charlotte (UNCC). The project has also established ongoing collaborations with the UNCC energy related centers of the Energy Production & Infrastructure Center (EPIC), the NC Motorsports and Automotive Research Center (NCMARC) and the Infrastructure, Design, Environment and Sustainability Center (IDEAS). The results of the project activities are presented as two major areas – (1) course and laboratory development, offerings and delivery, and (2) program recruitment, promotions and collaborations. Over the project period, the primary activity has been the development and offering of 11 HFCT courses and accompanying laboratories. This process has taken three years with the courses first being developed and then offered each year over the timeframe.

  8. Extended burnup demonstration: reactor fuel program. Pre-irradiation characterization and summary of pre-program poolside examinations. Big Rock Point extended burnup fuel

    International Nuclear Information System (INIS)

    This report is a resource document characterizing the 64 fuel rods being irradiated at the Big Rock Point reactor as part of the Extended Burnup Demonstration being sponsored jointly by the US Department of Energy, Consumers Power Company, Exxon Nuclear Company, and General Public Utilities. The program entails extending the exposure of standard BWR fuel to a discharge average of 38,000 MWD/MTU to demonstrate the feasibility of operating fuel of standard design to levels significantly above current limits. The fabrication characteristics of the Big Rock Point EBD fuel are presented along with measurement of rod length, rod diameter, pellet stack height, and fuel rod withdrawal force taken at poolside at burnups up to 26,200 MWD/MTU. A review of the fuel examination data indicates no performance characteristics which might restrict the continued irradiation of the fuel

  9. Temporal rhythm of petal programmed cell death in Ipomoea purpurea.

    Science.gov (United States)

    Gui, M-Y; Ni, X-L; Wang, H-B; Liu, W-Z

    2016-09-01

    Flowers are the main sexual reproductive organs in plants. The shapes, colours and scents of corolla of plant flowers are involved in attracting insect pollinators and increasing reproductive success. The process of corolla senescence was investigated in Ipomoea purpurea (Convolvulaceae) in this study. In the research methods of plant anatomy, cytology, cell chemistry and molecular biology were used. The results showed that at the flowering stage cells already began to show distortion, chromatin condensation, mitochondrial membrane degradation and tonoplast dissolution and rupture. At this stage genomic DNA underwent massive but gradual random degradation. However, judging from the shape and structure, aging characteristics did not appear until the early flower senescence stage. The senescence process was slow, and it was completed at the late stage of flower senescence with a withering corolla. We may safely arrive at the conclusion that corolla senescence of I. purpurea was mediated by programmed cell death (PCD) that occurred at the flowering stage. The corolla senescence exhibited an obvious temporal rhythm, which demonstrated a high degree of coordination with pollination and fertilization. PMID:27259176

  10. Waste management system functional requirements for Interim Waste Management Facilities (IWMFs) and technology demonstrations, LLWDDD [Low-Level Disposal Development and Demonstration] Program

    International Nuclear Information System (INIS)

    The purpose of this report is to build upon the preceding decisions and body of information to prepare draft system functional requirements for each classification of waste disposal currently proposed for Low-Level Waste Disposal Development Demonstration (LLWDDD) projects. Functional requirements identify specific information and data needs necessary to satisfy engineering design criteria/objectives for Interim Waste Management Facilities. This draft will suppor the alternatives evaluation process and will continue to evolve as strategy is implemented, regulatory limits are established, technical and economic uncertainties are resolved, and waste management plans are being implemented. This document will become the planning basis for the new generation of solid LLW management facilities on new sites on the Reservation. Eighteen (18) general system requirements are identified which are applicable to all four Low-Level Waste (LLW) disposal classifications. Each classification of LLW disposal is individually addressed with respect ot waste characteristics, site considerations, facility operations, facility closure/post-closure, intruder barriers, institutional control, and performance monitoring requirements. Three initial LLW disposal sites have been proposed as locations on the ORR for the first demonstrations

  11. Plant programmed cell death and the point of no return

    NARCIS (Netherlands)

    Doorn, van W.G.

    2005-01-01

    The point of no return during programmed cell death (PCD) is defined as the step beyond which the cell is irreversibly committed to die. Some plant cells can be saved before this point by inducing the formation of functional chloroplasts. A visibly senescent tissue will then become green again and l

  12. DoD Climate Change Fuel Cell Program

    Energy Technology Data Exchange (ETDEWEB)

    Ken Olsen

    2006-09-15

    This report discusses the first year of operation of a fuel cell power plant located at the Ocean County College, Toms River, New Jersey. PPL EnergyPlus, LLC installed the plant under a contract with Ocean County College. A DFC{reg_sign}300 fuel cell, manufactured by Fuel Cell Energy, Inc. of Danbury, CT was selected for the project. The fuel cell successfully operated from January 1, 2004 to December 31, 2004. This report discusses the performance of the plant during this period. Ocean County College's decision to contract for use of a fuel cell at the college reflects the institution's commitment to managing energy costs, exercising environmental leadership, and leveraging innovative technologies to accomplish its energy and environmental goals. Ocean County College's director of facilities was interested in finding new energy cost reduction opportunities that could build on the institution's growing reputation for commitment to energy efficiency and environmental quality while exploring new technologies. This combination of goals positioned Ocean County College to value the prospect of installing a fuel cell as a demonstration project that could deliver on its commitment. PPL EnergyPlus, LLC developed the project and Millennium Builders, a PPL company, was chosen as the general contractor for the project. PPL and Ocean County College worked very closely with Jersey Central Power and Light (JCP&L) and New Jersey Natural Gas (NJNG) Company to assure integration of the fuel cell with the local utilities. The 250 kW molten carbonate fuel cell (MCFC) and its balance of plant is contained in an all-weather container located just outside the college's Instructional Building on a cement pad in a fenced-in 30 x 50 foot area in close proximity to the college's boiler and electrical rooms. Cables and piping bring power and hot water from the fuel cell into these interior control areas. The unit's electrical output is fed onto the college

  13. A complete process for production of flexible large area polymer solar cells entirely using screen printing-First public demonstration

    DEFF Research Database (Denmark)

    Krebs, Frederik C; Jørgensen, Mikkel; Norrman, Kion;

    2009-01-01

    A complete polymer solar cell module prepared in the ambient atmosphere under industrial conditions is presented. The versatility of the polymer solar cell technology is demonstrated through the use of abstract forms for the active area, a flexible substrate, processing entirely from solution, co...

  14. FY 1994 program summary: Office of Technology Development, Office of Research and Development, Office of Demonstration, Testing, and Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    1994-10-01

    The US Department of Energy (DOE) Office of Environmental Management, formerly the Office of Environmental Restoration and Waste Management (EM), was established in November 1989 as the first step toward correcting contamination problems resulting from nearly 50 years of nuclear weapons production and fuel processing activities. EM consolidates several DOE organizations previously responsible for the handling, treatment, and disposition of radioactive and hazardous waste. Within EM, the Office of Technology Development (OTD/EM-50) is responsible for developing technologies to meet DOE`s goal for environmental restoration. OTD manages an aggressive national program of applied research, development, demonstration, testing, and evaluation (RDDT and E) for environmental cleanup, waste management, and related technologies. The program is designed to resolve major technical issues, to rapidly advanced beyond current technologies for environmental restoration and waste management operations, and to expedite compliance with applicable environmental laws and regulations. This report summarizes Fiscal Year 1994 (FY94) programmatic information, accomplishments, and planned activities relevant to the individual activities within OTD`s RDDT and E.

  15. FY 1994 program summary: Office of Technology Development, Office of Research and Development, Office of Demonstration, Testing, and Evaluation

    International Nuclear Information System (INIS)

    The US Department of Energy (DOE) Office of Environmental Management, formerly the Office of Environmental Restoration and Waste Management (EM), was established in November 1989 as the first step toward correcting contamination problems resulting from nearly 50 years of nuclear weapons production and fuel processing activities. EM consolidates several DOE organizations previously responsible for the handling, treatment, and disposition of radioactive and hazardous waste. Within EM, the Office of Technology Development (OTD/EM-50) is responsible for developing technologies to meet DOE's goal for environmental restoration. OTD manages an aggressive national program of applied research, development, demonstration, testing, and evaluation (RDDT and E) for environmental cleanup, waste management, and related technologies. The program is designed to resolve major technical issues, to rapidly advanced beyond current technologies for environmental restoration and waste management operations, and to expedite compliance with applicable environmental laws and regulations. This report summarizes Fiscal Year 1994 (FY94) programmatic information, accomplishments, and planned activities relevant to the individual activities within OTD's RDDT and E

  16. FY13 GLYCOLIC-NITRIC ACID FLOWSHEET DEMONSTRATIONS OF THE DWPF CHEMICAL PROCESS CELL WITH SIMULANTS

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, D.; Zamecnik, J.; Best, D.

    2014-03-13

    Savannah River Remediation is evaluating changes to its current Defense Waste Processing Facility flowsheet to replace formic acid with glycolic acid in order to improve processing cycle times and decrease by approximately 100x the production of hydrogen, a potentially flammable gas. Higher throughput is needed in the Chemical Processing Cell since the installation of the bubblers into the melter has increased melt rate. Due to the significant maintenance required for the safety significant gas chromatographs and the potential for production of flammable quantities of hydrogen, eliminating the use of formic acid is highly desirable. Previous testing at the Savannah River National Laboratory has shown that replacing formic acid with glycolic acid allows the reduction and removal of mercury without significant catalytic hydrogen generation. Five back-to-back Sludge Receipt and Adjustment Tank (SRAT) cycles and four back-to-back Slurry Mix Evaporator (SME) cycles were successful in demonstrating the viability of the nitric/glycolic acid flowsheet. The testing was completed in FY13 to determine the impact of process heels (approximately 25% of the material is left behind after transfers). In addition, back-to-back experiments might identify longer-term processing problems. The testing was designed to be prototypic by including sludge simulant, Actinide Removal Product simulant, nitric acid, glycolic acid, and Strip Effluent simulant containing Next Generation Solvent in the SRAT processing and SRAT product simulant, decontamination frit slurry, and process frit slurry in the SME processing. A heel was produced in the first cycle and each subsequent cycle utilized the remaining heel from the previous cycle. Lower SRAT purges were utilized due to the low hydrogen generation. Design basis addition rates and boilup rates were used so the processing time was shorter than current processing rates.

  17. Research, development and demonstration of a fuel cell/battery powered bus system. Phase 1, Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1990-02-28

    Purpose of the Phase I effort was to demonstrate feasibility of the fuel cell/battery system for powering a small bus (under 30 ft or 9 m) on an urban bus route. A brassboard powerplant was specified, designed, fabricated, and tested to demonstrate feasibility in the laboratory. The proof-of-concept bus, with a powerplant scaled up from the brassboard, will be demonstrated under Phase II.

  18. Music Generated by a Zn/Cu Electrochemical Cell, a Lemon Cell, and a Solar Cell: A Demonstration for General Chemistry

    Science.gov (United States)

    Cady, Susan G.

    2014-01-01

    The circuit board found in a commercial musical greeting card is used to supply music for electrochemical cell demonstrations. Similar to a voltmeter, the "modified" musical device is connected to a chemical reaction that produces electricity. The commercial 1 V battery inside the greeting card circuit board can be replaced with an…

  19. Program description for the program Demonstration program for electric vehicles 2011-2015; Programbeskrivning foer programmet Demonstrationsprogram foer elfordon 2011-2015

    Energy Technology Data Exchange (ETDEWEB)

    2011-04-15

    The program's efforts aim at, firstly, from a user perspective identify and possibly eliminate barriers for large-scale introduction of electric vehicles on the Swedish market. Through various research activities based in the real environment experience may be drawn from both vehicle use and different types of behavior. Infrastructure tests on a sufficient scale give the combined effect of real-life test environments for the combination of vehicles and charging environments as well as a base for experiences of softer issues around electric vehicles. Precisely these behavioral experiences are difficult to reach via the related but more technically focused programs such as, FFI (Vehicle strategic research and innovation) and Energy efficient road vehicles. Several systems for electrification of vehicles exist today, and further initiatives for solutions are on the way. It is therefore very important to examine which systems give the highest total energy efficiency. It is also relevant for these different systems in a real environment to explore the potentials and difficulties that may arise in larger implementations

  20. Early Site Permit Demonstration Program: Siting Guide, Site selection and evaluation criteria for an early site permit application. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    1993-03-24

    In August 1991, the Joint Contractors came to agreement with Sandia National Laboratories (SNL) and the Department of Energy (DOE) on a workscope for the cost-shared Early Site Permit Demonstration Program. One task within the scope was the development of a guide for site selection criteria and procedures. A generic Siting Guide his been prepared that is a roadmap and tool for applicants to use developing detailed siting plans for their specific region of the country. The guide presents three fundamental principles that, if used, ensure a high degree of success for an ESP applicant. First, the site selection process should take into consideration environmentally diverse site locations within a given region of interest. Second, the process should contain appropriate opportunities for input from the public. Third, the process should be applied so that it is clearly reasonable to an impartial observer, based on appropriately selected criteria, including criteria which demonstrate that the site can host an advanced light water reactor (ALWR). The Siting Guide provides for a systematic, comprehensive site selection process in which three basic types of criteria (exclusionary, avoidance, and suitability) are presented via a four-step procedure. It provides a check list of the criteria for each one of these steps. Criteria are applied qualitatively, as well as presented numerically, within the guide. The applicant should use the generic guide as an exhaustive checklist, customizing the guide to his individual situation.

  1. Early Site Permit Demonstration Program: Siting Guide, Site selection and evaluation criteria for an early site permit application

    International Nuclear Information System (INIS)

    In August 1991, the Joint Contractors came to agreement with Sandia National Laboratories (SNL) and the Department of Energy (DOE) on a workscope for the cost-shared Early Site Permit Demonstration Program. One task within the scope was the development of a guide for site selection criteria and procedures. A generic Siting Guide his been prepared that is a roadmap and tool for applicants to use developing detailed siting plans for their specific region of the country. The guide presents three fundamental principles that, if used, ensure a high degree of success for an ESP applicant. First, the site selection process should take into consideration environmentally diverse site locations within a given region of interest. Second, the process should contain appropriate opportunities for input from the public. Third, the process should be applied so that it is clearly reasonable to an impartial observer, based on appropriately selected criteria, including criteria which demonstrate that the site can host an advanced light water reactor (ALWR). The Siting Guide provides for a systematic, comprehensive site selection process in which three basic types of criteria (exclusionary, avoidance, and suitability) are presented via a four-step procedure. It provides a check list of the criteria for each one of these steps. Criteria are applied qualitatively, as well as presented numerically, within the guide. The applicant should use the generic guide as an exhaustive checklist, customizing the guide to his individual situation

  2. Induction of multiple programmed cell death pathways by IFN-beta in human non-small-cell lung cancer cell lines.

    Science.gov (United States)

    Zhang, H; Koty, P P; Mayotte, J; Levitt, M L

    1999-02-25

    Tissue transglutaminase (tTG) and keratinocyte transglutaminase (kTG), as well as the cross-linked envelopes (CLE) that they form, have been associated with squamous differentiation and programmed cell death in epithelial cells. When interferon-beta (IFN-beta) was used to stimulate differentiation and programmed cell death in the human lung cancer cell lines NCI-H596 and NCI-H226, the cells underwent a decrease in cellular density. In NCI-H596 IFN-beta caused an increase in kTG activity and DNA fragmentation in the lower density cells, which were significantly slower growing than control cells. However, in the higher density cells, which were only slightly slower growing than control cells, IFN-beta caused an increase in tTG activity and CLE competence. Dual-parameter flow cytometry demonstrated that IFN-beta-induced squamous differentiation preceded programmed cell death. Treatment of NCI-H596 cells with monodansylcadaverine, a transglutaminase inhibitor, prevented the increase in CLE competence, but did not inhibit DNA fragmentation. These results suggest that IFN-beta can induce NCI-H596 cells to enter multiple cell death pathways and that these pathways are not only differentiation related, but may also be growth driven. PMID:10047455

  3. ENVIRONMENTAL TECHNOLOGY VERIFICATION (ETV) PROGRAM: FUEL CELLS

    Science.gov (United States)

    The U.S. Environmental Protection Agency (EPA) Environmental Technology Verification (ETV) Program evaluates the performance of innovative air, water, pollution prevention and monitoring technologies that have the potential to improve human health and the environment. This techno...

  4. Mitochondrial Dynamics Controls T Cell Fate through Metabolic Programming.

    Science.gov (United States)

    Buck, Michael D; O'Sullivan, David; Klein Geltink, Ramon I; Curtis, Jonathan D; Chang, Chih-Hao; Sanin, David E; Qiu, Jing; Kretz, Oliver; Braas, Daniel; van der Windt, Gerritje J W; Chen, Qiongyu; Huang, Stanley Ching-Cheng; O'Neill, Christina M; Edelson, Brian T; Pearce, Edward J; Sesaki, Hiromi; Huber, Tobias B; Rambold, Angelika S; Pearce, Erika L

    2016-06-30

    Activated effector T (TE) cells augment anabolic pathways of metabolism, such as aerobic glycolysis, while memory T (TM) cells engage catabolic pathways, like fatty acid oxidation (FAO). However, signals that drive these differences remain unclear. Mitochondria are metabolic organelles that actively transform their ultrastructure. Therefore, we questioned whether mitochondrial dynamics controls T cell metabolism. We show that TE cells have punctate mitochondria, while TM cells maintain fused networks. The fusion protein Opa1 is required for TM, but not TE cells after infection, and enforcing fusion in TE cells imposes TM cell characteristics and enhances antitumor function. Our data suggest that, by altering cristae morphology, fusion in TM cells configures electron transport chain (ETC) complex associations favoring oxidative phosphorylation (OXPHOS) and FAO, while fission in TE cells leads to cristae expansion, reducing ETC efficiency and promoting aerobic glycolysis. Thus, mitochondrial remodeling is a signaling mechanism that instructs T cell metabolic programming. PMID:27293185

  5. DNAM-1 Expression Marks an Alternative Program of NK Cell Maturation

    Directory of Open Access Journals (Sweden)

    Ludovic Martinet

    2015-04-01

    Full Text Available Natural killer (NK cells comprise a heterogeneous population of cells important for pathogen defense and cancer surveillance. However, the functional significance of this diversity is not fully understood. Here, we demonstrate through transcriptional profiling and functional studies that the activating receptor DNAM-1 (CD226 identifies two distinct NK cell functional subsets: DNAM-1+ and DNAM-1− NK cells. DNAM-1+ NK cells produce high levels of inflammatory cytokines, have enhanced interleukin 15 signaling, and proliferate vigorously. By contrast, DNAM-1− NK cells that differentiate from DNAM-1+ NK cells have greater expression of NK-cell-receptor-related genes and are higher producers of MIP1 chemokines. Collectively, our data reveal the existence of a functional program of NK cell maturation marked by DNAM-1 expression.

  6. Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens

    OpenAIRE

    Takami Matsuyama; Yoshito Eizuru; Takuro Kanekura; Yoshifumi Kawano; Shuji Izumo; Xinshan Jia; Katsuyuki Aozasa; Taku Nagai; Jia Wang; Kazuhisa Hasui

    2012-01-01

    Immunohistochemistry (IHC) for detecting key signal molecules involved in programmed cell death (PCD) in archival human pathology specimens is fairly well established. Detection of cleaved caspase-3 in lymphocytes in rheumatoid arthritis (RA) and gastric surface foveolar glandular epithelia but not in synoviocytes in RA, gastric fundic glandular epithelia, or nasal NK/T-cell lymphoma (NKTCL) cells suggests anti-apoptotic mechanisms in cell differentiation and in oncogenesis such as the induct...

  7. CIF2Cell: Generating geometries for electronic structure programs

    Science.gov (United States)

    Björkman, Torbjörn

    2011-05-01

    The CIF2Cell program generates the geometrical setup for a number of electronic structure programs based on the crystallographic information in a Crystallographic Information Framework (CIF) file. The program will retrieve the space group number, Wyckoff positions and crystallographic parameters, make a sensible choice for Bravais lattice vectors (primitive or principal cell) and generate all atomic positions. Supercells can be generated and alloys are handled gracefully. The code currently has output interfaces to the electronic structure programs ABINIT, CASTEP, CPMD, Crystal, Elk, Exciting, EMTO, Fleur, RSPt, Siesta and VASP. Program summaryProgram title: CIF2Cell Catalogue identifier: AEIM_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEIM_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: GNU GPL version 3 No. of lines in distributed program, including test data, etc.: 12 691 No. of bytes in distributed program, including test data, etc.: 74 933 Distribution format: tar.gz Programming language: Python (versions 2.4-2.7) Computer: Any computer that can run Python (versions 2.4-2.7) Operating system: Any operating system that can run Python (versions 2.4-2.7) Classification: 7.3, 7.8, 8 External routines: PyCIFRW [1] Nature of problem: Generate the geometrical setup of a crystallographic cell for a variety of electronic structure programs from data contained in a CIF file. Solution method: The CIF file is parsed using routines contained in the library PyCIFRW [1], and crystallographic as well as bibliographic information is extracted. The program then generates the principal cell from symmetry information, crystal parameters, space group number and Wyckoff sites. Reduction to a primitive cell is then performed, and the resulting cell is output to suitably named files along with documentation of the information source generated from any bibliographic information contained in the CIF

  8. An Audiovisual Program in Cell Biology

    Science.gov (United States)

    Fedoroff, Sergey; Opel, William

    1978-01-01

    A subtopic of cell biology, the structure and function of cell membranes, has been developed as a series of seven self-instructional slide-tape units and tested in five medical schools. Organization of advisers, analysis and definition of objectives and content, and development and evaluation of scripts and storyboards are discussed. (Author/LBH)

  9. Genetic regulation of programmed cell death in Drosophila

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Programmed cell death plays an important role in maintaining homeostasis during animal development, and has been conserved in animals as different as nematodes and humans. Recent studies of Drosophila have provided valuable information toward our understanding of genetic regulation of death. Different signals trigger the novel death regulators rpr, hid, and grim, that utilize the evolutionarily conserved iap and ark genes to modulate caspase function. Subsequent removal of dying cells also appears to be accomplished by conserved mechanisms. The similarity between Drosophila and human in cell death signaling pathways illustrate the promise of fruit flies as a model system to elucidate the mechanisms underlying regulation of programmed cell death.

  10. Final proceedings of the second solar heating and cooling commercial demonstration program contractors' review. Volume 1. Summary and analysis

    Energy Technology Data Exchange (ETDEWEB)

    1979-07-01

    The Second Solar Heating and Cooling Commercial Demonstration Program Contractors' Review was attended by over 300 representatives from demonstration projects, the Department of Energy (DOE), other Federal agencies, and Government contractors. This volume presents a thorough study of the 140 project papers printed in the three-volume proceedings. A comprehensive table describing the 140 sites is included. This table presents a description of the sites not only in terms of equipment and performance, but also those regarding their problems, successes, and other experiences. The panel discussions on demonstration projects, the National Solar Data Program and the Commercial Demonstration Program are summarized and analyzed. The results of a special survey of Commercial Demonstration Program contractors are presented. Finally, the results of a polling of Review participants on the effectiveness of the meeting are detailed. (WHK)

  11. Argyrophilic cells in the larynx of the guinea-pig demonstrated by the method of Grimelius

    DEFF Research Database (Denmark)

    Kirkeby, S; Romert, P

    1977-01-01

    Argyrophilic cells with branching processes are shown in both the surface epithelium and in the glands in the larynx of adult guinea-pigs using the Grimelius silver technique after GPA fixation. When Bouin's fixative or neutral formalin were used as fixatives argrophilic cells could not be identi......Argyrophilic cells with branching processes are shown in both the surface epithelium and in the glands in the larynx of adult guinea-pigs using the Grimelius silver technique after GPA fixation. When Bouin's fixative or neutral formalin were used as fixatives argrophilic cells could...

  12. Programmed cell death and its role in inflammation

    Institute of Scientific and Technical Information of China (English)

    Yong Yang; Ge-Ning Jiang; Peng Zhang; Jie Fan

    2015-01-01

    Cell death plays an important role in the regulation of inflammation and may be the result of inflammation. The maintenance of tissue homeostasis necessitates both the recognition and removal of invading microbial pathogens as well as the clearance of dying cells. In the past few decades, emerging knowledge on cell death and inflammation has enriched our molecular understanding of the signaling pathways that mediate various programs of cell death and multiple types of inflammatory responses. This review provides an overview of the major types of cell death related to inflammation. Modification of cell death pathways is likely to be a logical therapeutic target for inflammatory diseases.

  13. Quantum algorithm for programmed cell death of Caenorhabditis elegans

    International Nuclear Information System (INIS)

    During the development of Caenorhabditis elegans, through cell divisions, a total of exactly 1090 cells are generated, 131 of which undergo programmed cell death (PCD) to result in an adult organism comprising 959 cells. Of those 131, exactly 113 undergo PCD during embryogenesis, subdivided across the cell lineages in the following fashion: 98 for AB lineage; 14 for MS lineage; and 1 for C lineage. Is there a law underlying these numbers, and if there is, what could it be? Here we wish to show that the count of the cells undergoing PCD complies with the cipher laws related to the algorithms of Shor and of Grover

  14. Treatment plan for aqueous/organic/decontamination wastes under the Oak Ridge Reservation FFCA Development, Demonstration, Testing, and Evaluation Program

    International Nuclear Information System (INIS)

    The U.S. Department of Energy (DOE) Oak Ridge Operations Office and the U.S. Environmental Protection Agency (EPA)-Region IV have entered into a Federal Facility Compliance Agreement (FFCA) which seeks to facilitate the treatment of low-level mixed wastes currently stored at the Oak Ridge Reservation (ORR) in violation of the Resource, Conservation and Recovery Act Land Disposal Restrictions. The FFCA establishes schedules for DOE to identify treatment for wastes, referred to as Appendix B wastes, that current have no identified or existing capacity for treatment. A development, demonstration, testing, and evaluation (DDT ampersand E) program was established to provide the support necessary to identify treatment methods for mixed was meeting the Appendix B criteria. The Program has assembled project teams to address treatment development needs for major categories of the Appendix B wastes based on the waste characteristics and possible treatment technologies. The Aqueous, Organic, and Decontamination (A OE D) project team was established to identify pretreatment options for aqueous and organic wastes which will render the waste acceptable for treatment in existing waste treatment facilities and to identify the processes to decontaminate heterogeneous debris waste. In addition, the project must also address the treatment of secondary waste generated by other DDT ampersand E projects. This report details the activities to be performed under the A OE D Project in support of the identification, selection, and evaluation of treatment processes. The goals of this plan are (1) to determine the major aqueous and organic waste streams requiring treatment, (2) to determine the treatment steps necessary to make the aqueous and organic waste acceptable for treatment in existing treatment facilities on the ORR or off-site, and (3) to determine the processes necessary to decontaminate heterogeneous wastes that are considered debris

  15. Treatment plan for aqueous/organic/decontamination wastes under the Oak Ridge Reservation FFCA Development, Demonstration, Testing, and Evaluation Program

    Energy Technology Data Exchange (ETDEWEB)

    Backus, P.M.; Benson, C.E.; Gilbert, V.P.

    1994-08-01

    The U.S. Department of Energy (DOE) Oak Ridge Operations Office and the U.S. Environmental Protection Agency (EPA)-Region IV have entered into a Federal Facility Compliance Agreement (FFCA) which seeks to facilitate the treatment of low-level mixed wastes currently stored at the Oak Ridge Reservation (ORR) in violation of the Resource, Conservation and Recovery Act Land Disposal Restrictions. The FFCA establishes schedules for DOE to identify treatment for wastes, referred to as Appendix B wastes, that current have no identified or existing capacity for treatment. A development, demonstration, testing, and evaluation (DDT&E) program was established to provide the support necessary to identify treatment methods for mixed was meeting the Appendix B criteria. The Program has assembled project teams to address treatment development needs for major categories of the Appendix B wastes based on the waste characteristics and possible treatment technologies. The Aqueous, Organic, and Decontamination (A/O/D) project team was established to identify pretreatment options for aqueous and organic wastes which will render the waste acceptable for treatment in existing waste treatment facilities and to identify the processes to decontaminate heterogeneous debris waste. In addition, the project must also address the treatment of secondary waste generated by other DDT&E projects. This report details the activities to be performed under the A/O/D Project in support of the identification, selection, and evaluation of treatment processes. The goals of this plan are (1) to determine the major aqueous and organic waste streams requiring treatment, (2) to determine the treatment steps necessary to make the aqueous and organic waste acceptable for treatment in existing treatment facilities on the ORR or off-site, and (3) to determine the processes necessary to decontaminate heterogeneous wastes that are considered debris.

  16. DEMONSTRATION OF FUEL CELLS TO RECOVER ENERGY FROM LANDFILL GAS - PHASE I FINAL REPORT: CONCEPTUAL STUDY

    Science.gov (United States)

    The report discusses results of a conceptual design, cost, and evaluation study of energy recovery from landfill gas using a commercial phosphoric acid fuel cell power plant. The conceptual design of the fuel cell energy recovery system is described, and its economic and environm...

  17. 2014 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2014-11-01

    The 2014 Annual Progress Report summarizes fiscal year 2014 activities and accomplishments by projects funded by the DOE Hydrogen Program. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing; technology validation; safety, codes and standards; market transformation; and systems analysis.

  18. 2011 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    Satyapal, Sunita [Office of Energy Efficiency and Renewable Energy (EERE), Washington, DC (United States)

    2011-11-01

    The 2011 Annual Progress Report summarizes fiscal year 2011 activities and accomplishments by projects funded by the DOE Hydrogen Program. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing; technology validation; safety, codes and standards; education; market transformation; and systems analysis.

  19. 2013 Annual Progress Report: DOE Hydrogen and Fuel Cells Program

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2013-12-01

    The 2013 Annual Progress Report summarizes fiscal year 2013 activities and accomplishments by projects funded by the DOE Hydrogen Program. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing; technology validation; safety, codes and standards; market transformation; and systems analysis.

  20. Stability of RNA and DNA in Bone Marrow Cells, Demonstrated with Tritiated Cytidine and Thymidine

    International Nuclear Information System (INIS)

    DNA and RNA metabolism was studied using tritiated thymidine (H3Th), a specific precursor for DNA, and tritiated cytidine (H3C), a common precursor for both RNA and DNA. With H3C, differential incorporation into RNA, DNA or the soluble pool was determined autoradiographically in the single cell, and/or chemically for cell populations by means of differential extraction using appropriate treatment with perchloric acid. Initial turnover studies in the Hela cell with H3C indicated the precursor role of nuclear RNA for cytoplasmic RNA. Conservation and distribution of label in the RNA fraction was consistent with major macromolecular RNA stability, and continued incorporation of label into the DNA fraction was consistent with the presence of a late precursor for DNA. Similar findings were observed in the immature bone marrow cells of the rat studied over a period of several days after intravenous administration of H3C. The amount of tritium activity in the acid-soluble' RNA and DNA fractions was followed chemically and/or autoradiographically. The three curves were found to be parallel from the first day after injection and parallel to curves for tritium label in DNA following H3Th administration. The expected rate of fall off in label, calculated from kinetics of the rat bone marrow cell populations studied separately by H3Th and autoradiography, assuming no turnover of RNA or DNA and loss of label only by loss of marrow cells by division and maturation, was in agreement with the slopes obtained. The results indicate that, once synthesized, soluble and macromolecular RNA is retained by the bone marrow cell in a manner similar to DNA. Newly formed RNA and DNA are diluted in the cells only through cell division. (author)

  1. Solar cells. Danish strategy for research, development, demonstration and distribution. Part 1 - Solar cell strategy 2010; Solceller. Dansk strategi for forskning, udvikling, demonstration og udbredelse. Del I - Solcellestrategi 2010

    Energy Technology Data Exchange (ETDEWEB)

    2009-07-01

    The areas for strategic action within Danish solar cells R and D range from a developmental effort that can take effect in a relatively short term to longer-term research and development efforts aimed at increasing solar cell efficiency and the development of new building components with integrated photovoltaic systems. In non-priority order the following actions are recommended: A. Silicon solar cells / modules. Development of silicon materials, solar cells and modules to the extent that a Danish effort is likely to have value and impact both nationally and internationally. B. New advanced solar cell types such as PEC, polymer cells and nano-structured cells. This is long term R and D, which is directed at producing solar cells that are significantly cheaper than today's solar cells. C. Power Conversion - inverter technology. Power conversion by inverters are an important part of grid-connected photovoltaic systems. D. System technology, including the integration into the power system. Grid-connected photovoltaic systems in Denmark are expected in the near future primarily to decentralized, often placed in each building as an integral part of the building's power installation and coupled to the low voltage grid. E. Photovoltaics in buildings (BIPV). Building integrated photovoltaic systems is expected to be a key area for development of the use of photovoltaic systems. F. Design and aesthetics, especially regarding multi-functional building components. This will be a special development area for building integrated solutions. G. Demonstration activities. Includes demonstration projects and activities that will maintain general Danish interest in solar cell technology and promote the transition from R and D to industrial processes, supporting Danish products and helping to market maturation. H. Dissemination activities. I. International cooperation. Participation in international cooperation in the solar cell field, including standardization work, IEA, EU

  2. Report for the ASC CSSE L2 Milestone (4873) - Demonstration of Local Failure Local Recovery Resilient Programming Model.

    Energy Technology Data Exchange (ETDEWEB)

    Heroux, Michael Allen; Teranishi, Keita

    2014-06-01

    Recovery from process loss during the execution of a distributed memory parallel application is presently achieved by restarting the program, typically from a checkpoint file. Future computer system trends indicate that the size of data to checkpoint, the lack of improvement in parallel file system performance and the increase in process failure rates will lead to situations where checkpoint restart becomes infeasible. In this report we describe and prototype the use of a new application level resilient computing model that manages persistent storage of local state for each process such that, if a process fails, recovery can be performed locally without requiring access to a global checkpoint file. LFLR provides application developers with an ability to recover locally and continue application execution when a process is lost. This report discusses what features are required from the hardware, OS and runtime layers, and what approaches application developers might use in the design of future codes, including a demonstration of LFLR-enabled MiniFE code from the Matenvo mini-application suite.

  3. Soils, surficial geology, and geomorphology of the Bear Creek Valley Low-Level Waste Disposal Development and Demonstration Program site

    Energy Technology Data Exchange (ETDEWEB)

    Lietzke, D.A.; Lee, S.Y.; Lambert, R.E.

    1988-04-01

    An intensive soil survey was conducted on the proposed Low-Level Waste Disposal Development and Demonstration Program site (LLWDDD) in Bear Creek Valley. Soils on the site were related to the underlying residuum and to the surficial colluvium and alluvium. Within any particular geologic formation, soils were subdivided based mostly on the degree of weathering, as reflected by saprolite weathering and morphologic features of the soils. Degree of weathering was related both to slope shape and gradient and to the joint-fracture system. Erosion classes were also used to make further subdivisions of any particular soil. Deep pits were dug in each of the major Conasauga Group formations (Pumpkin Valley, Rogersville, Maryville, and Nolichucky) for soil and saprolite characterization. Because of the widespread presence of alluvium and colluvium, which are potential sources of fill and final cover material, pits and trenches were dug to characterize the properties of these soils and to try to understand the past geomorphic history of the site. The results of the soil survey investigation indicated that the deeply weathered Pumpkin Valley residuum has good potential for the construction of tumuli or other types of belowground or aboveground burial of prepackaged compacted waste. 11 refs., 30 figs., 3 tabs.

  4. Soils, surficial geology, and geomorphology of the Bear Creek Valley Low-Level Waste Disposal Development and Demonstration Program site

    International Nuclear Information System (INIS)

    An intensive soil survey was conducted on the proposed Low-Level Waste Disposal Development and Demonstration Program site (LLWDDD) in Bear Creek Valley. Soils on the site were related to the underlying residuum and to the surficial colluvium and alluvium. Within any particular geologic formation, soils were subdivided based mostly on the degree of weathering, as reflected by saprolite weathering and morphologic features of the soils. Degree of weathering was related both to slope shape and gradient and to the joint-fracture system. Erosion classes were also used to make further subdivisions of any particular soil. Deep pits were dug in each of the major Conasauga Group formations (Pumpkin Valley, Rogersville, Maryville, and Nolichucky) for soil and saprolite characterization. Because of the widespread presence of alluvium and colluvium, which are potential sources of fill and final cover material, pits and trenches were dug to characterize the properties of these soils and to try to understand the past geomorphic history of the site. The results of the soil survey investigation indicated that the deeply weathered Pumpkin Valley residuum has good potential for the construction of tumuli or other types of belowground or aboveground burial of prepackaged compacted waste. 11 refs., 30 figs., 3 tabs

  5. Environmental considerations for inclusion in program opportunity notices, requests for proposals, and environmental reports: fossil energy demonstration plants

    Energy Technology Data Exchange (ETDEWEB)

    Salk, M.S.; Baird, J.N.; Barnthouse, L.W.; Berry, L.G.; Roop, R.D.; Sanders, F.S.

    1978-01-01

    This document was prepared for the Department of Energy, Division of Coal Conversion, for use in developing Program Opportunity Notices (PON), Requests for Proposals (RFP), and Environmental Reports (ER). Environmental considerations to be addressed by Offerors and Contractors are divided into three parts that correspond to the separate stages of project development: (1) the Offeror's response to the PON or RFP; (2) the Contractor's performance of baseline data collection and environmental impact assessment that results in an Environmental Report (ER) for use by DOE in the preparation of an Environmental Impact Statement (EIS); and (3) the Contractor's continuance of environmental monitoring and assessment during construction and operation. Environmental analysis and environmental protection are an integral part of the Offeror's or Contractor's performance under contract to DOE. Consideration should be given to the health and socioeconomic status of man as well as to the physical and biological environment. The Offeror's or Contractor's consideration of and commitment to avoiding adverse environmental impacts must be demonstrated at every stage of the process from planning through construction and operation.

  6. Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

    OpenAIRE

    Podolsky, D K; Fournier, D A; Lynch, K E

    1986-01-01

    We studied glycoprotein content of human colonic goblet cells, using a library of monoclonal antibodies (MAbs) directed against purified human colonic mucin (HCM). Using indirect immunofluorescence (IIF), we found that 17 of 23 anti-HCM MAbs stained some or all goblet cells of normal human colonic mucosa. We observed a variety of cellular staining patterns, including (a) diffuse (homogeneous) staining of intracellular mucin, (b) speckled (inhomogeneous) staining of mucin droplets, (c) periphe...

  7. Osteoclast cytomorphometry demonstrates an abnormal population in B cell malignancies but not in multiple myeloma.

    Science.gov (United States)

    Chappard, D; Rossi, J F; Bataille, R; Alexandre, C

    1991-01-01

    Increased bone resorption in the vicinity of myeloma cells is mediated by local stimulating factors. Other malignancies of the B cell lineage are also able to produce resorbing factors responsible for increased bone resorption. We have studied three groups of subjects: 10 patients with overt multiple myeloma, 10 patients with a B cell malignancy, and 10 healthy human subjects as controls. Patients were studied at the time of diagnosis and had a transiliac bone biopsy. Osteoclasts were evident on histological sections by their acid phosphatase activity. A software was developed on an automatic image analyzer (Leitz TAS+) for measuring the maximal Feret's diameter (Oc.Le) of each osteoclast (corresponding to the osteoclast length). The histogram of Oc.Le frequency distribution was supplied in each group. In myeloma patients, the Oc.Le frequency distribution was similar to that in normal subjects and showed the histogram to be asymetric with a positive skew (maximum peak at 20-25 microns). With a graphical analysis, this distribution was shown to follow a lognormal distribution corresponding to a homogeneous osteoclast population. In other B cell malignancies, Oc.Le displayed a bimodal distribution with a peak at 20-25 microns and a lower peak at 10-15 microns. The graphical analysis showed that small (mononucleated?) osteoclasts are present in B cell malignancies with normal osteoclasts. This might reflect the secretion of different soluble factors by malignant cells of the B lymphocyte lineage. PMID:1706639

  8. Wheat enolase demonstrates potential as a non-toxic cryopreservation agent for liver and pancreatic cells.

    Science.gov (United States)

    Grondin, Mélanie; Chow-Shi-Yée, Mélanie; Ouellet, François; Averill-Bates, Diana A

    2015-05-01

    Cryopreservation is essential for long-term storage of cells and tissues, which can be used for clinical applications such as drug toxicity testing, human transplantation, reproductive, regenerative and transfusion medicine. It requires use of cryoprotectants (e.g. dimethyl disulfoxide (DMSO), glycerol) that protect cells and tissues from dehydration and damage caused by formation of intracellular ice during freezing. As an alternative to these cytotoxic cryoprotectants, we are developing new technology using natural substances produced by plants that survive freezing conditions. We previously showed that soluble protein extracts such as wheat protein extract (WPE) prepared from winter wheat plants can substitute for DMSO as a cryoprotectant for certain mammalian cell types. To identify novel cryoactive proteins, WPE was separated using different chromatographic procedures and cryoactive fractions were analyzed by mass spectrometry. The analysis revealed enolase as a potential wheat protein candidate. A recombinant enolase protein was prepared and was able to successfully cryopreserve rat hepatocytes and insulin-secreting INS832/13 pancreatic cells. Post-thaw cells had high viability and levels of metabolic activities. Cryopreserved cells were plateable and had good adherence and morphological properties. These results indicate that individual plant proteins such as enolase have promising potential as new, non-toxic technology for cryopreservation protocols used for clinical applications. PMID:25740431

  9. Chemical- and pathogen-induced programmed cell death in plants

    NARCIS (Netherlands)

    Iakimova, E.T.; Atanassov, A.; Woltering, E.J.

    2005-01-01

    This review focuses on recent update in the understanding of programmed cell death regarding the differences and similarities between the diverse types of cell death in animal and plant systems and describes the morphological and some biochemical determinants. The role of PCD in plant development an

  10. Fuel cell energy service Enron`s commerical program

    Energy Technology Data Exchange (ETDEWEB)

    Jacobson, M.W.

    1996-04-01

    Enron, the premier provider of clean fuels worldwide, has launched a unique energy service based on fuel cell technology. The goal of this program is to bring the benefits of fuel cell power to the broad commercial marketplace. Enron`s Energy Service is currently based on a 200 kilowatt phosphoric acid power plant manufactured by ONSI Corporation. This plant is fueled by natural gas or propane, and exhibits superior performance. Enron offers a `no hassle` package that provides customers with immediate benefits with no upfront capital or technical risks. This paper describes Enron`s fuel cell commercial program.

  11. Demonstration of the presence of the "deleted" MIR122 gene in HepG2 cells.

    Science.gov (United States)

    Hamad, Ibrahim A Y; Fei, Yue; Kalea, Anastasia Z; Yin, Dan; Smith, Andrew J P; Palmen, Jutta; Humphries, Steve E; Talmud, Philippa J; Walker, Ann P

    2015-01-01

    MicroRNA 122 (miR-122) is highly expressed in the liver where it influences diverse biological processes and pathways, including hepatitis C virus replication and metabolism of iron and cholesterol. It is processed from a long non-coding primary transcript (~7.5 kb) and the gene has two evolutionarily-conserved regions containing the pri-mir-122 promoter and pre-mir-122 hairpin region. Several groups reported that the widely-used hepatocytic cell line HepG2 had deficient expression of miR-122, previously ascribed to deletion of the pre-mir-122 stem-loop region. We aimed to characterise this deletion by direct sequencing of 6078 bp containing the pri-mir-122 promoter and pre-mir-122 stem-loop region in HepG2 and Huh-7, a control hepatocytic cell line reported to express miR-122, supported by sequence analysis of cloned genomic DNA. In contrast to previous findings, the entire sequence was present in both cell lines. Ten SNPs were heterozygous in HepG2 indicating that DNA was present in two copies. Three validation isolates of HepG2 were sequenced, showing identical genotype to the original in two, whereas the third was different. Investigation of promoter chromatin status by FAIRE showed that Huh-7 cells had 6.2 ± 0.19- and 2.7 ± 0.01- fold more accessible chromatin at the proximal (HNF4α-binding) and distal DR1 transcription factor sites, compared to HepG2 cells (p=0.03 and 0.001, respectively). This was substantiated by ENCODE genome annotations, which showed a DNAse I hypersensitive site in the pri-mir-122 promoter in Huh-7 that was absent in HepG2 cells. While the origin of the reported deletion is unclear, cell lines should be obtained from a reputable source and used at low passage number to avoid discrepant results. Deficiency of miR-122 expression in HepG2 cells may be related to a relative deficiency of accessible promoter chromatin in HepG2 versus Huh-7 cells. PMID:25811611

  12. Demonstration of the presence of the "deleted" MIR122 gene in HepG2 cells.

    Directory of Open Access Journals (Sweden)

    Ibrahim A Y Hamad

    Full Text Available MicroRNA 122 (miR-122 is highly expressed in the liver where it influences diverse biological processes and pathways, including hepatitis C virus replication and metabolism of iron and cholesterol. It is processed from a long non-coding primary transcript (~7.5 kb and the gene has two evolutionarily-conserved regions containing the pri-mir-122 promoter and pre-mir-122 hairpin region. Several groups reported that the widely-used hepatocytic cell line HepG2 had deficient expression of miR-122, previously ascribed to deletion of the pre-mir-122 stem-loop region. We aimed to characterise this deletion by direct sequencing of 6078 bp containing the pri-mir-122 promoter and pre-mir-122 stem-loop region in HepG2 and Huh-7, a control hepatocytic cell line reported to express miR-122, supported by sequence analysis of cloned genomic DNA. In contrast to previous findings, the entire sequence was present in both cell lines. Ten SNPs were heterozygous in HepG2 indicating that DNA was present in two copies. Three validation isolates of HepG2 were sequenced, showing identical genotype to the original in two, whereas the third was different. Investigation of promoter chromatin status by FAIRE showed that Huh-7 cells had 6.2 ± 0.19- and 2.7 ± 0.01- fold more accessible chromatin at the proximal (HNF4α-binding and distal DR1 transcription factor sites, compared to HepG2 cells (p=0.03 and 0.001, respectively. This was substantiated by ENCODE genome annotations, which showed a DNAse I hypersensitive site in the pri-mir-122 promoter in Huh-7 that was absent in HepG2 cells. While the origin of the reported deletion is unclear, cell lines should be obtained from a reputable source and used at low passage number to avoid discrepant results. Deficiency of miR-122 expression in HepG2 cells may be related to a relative deficiency of accessible promoter chromatin in HepG2 versus Huh-7 cells.

  13. Gas-phase decontamination demonstration on PORTS cell X-25-4-2. Final technology status report

    International Nuclear Information System (INIS)

    The Long-Term, Low Temperature (LTLT) process is a gas-phase in situ decontamination technique which has been tested by LMES/K-25 personnel on the laboratory scale with promising results. The purpose of the Gas-Phase Decontamination Demonstration at PORTS was to evaluate the LTLT process on an actual diffusion cascade cell at conditions similar to those used in the laboratory testing. The demonstration was conducted on PORTS diffusion cell X-25-4-2 which was one of the X-326 Building cells which was permanently shutdown as part of the Suspension of HEU Production at PORTS. The demonstration full-scale test consisted of rendering the cell leak-tight through the installation of Dresser seals onto the process seals, exposing the cell to the oxidants ClF3 and F2 for a period of 105 days and evaluating the effect of the clean-up treatment on cell samples and coupons representing the major diffusion cascade materials of construction. The results were extrapolated to determine the effectiveness of LTLT decontamination over the range of historical uranium isotope assays present in the diffusion complex. It was determined that acceptable surface contamination levels could be obtained in all of the equipment in the lower assay cascades which represents the bulk of the equipment contained in the diffusion complex

  14. Antiproliferative activity of Eremanthus crotonoides extracts and centratherin demonstrated in brain tumor cell lines

    Directory of Open Access Journals (Sweden)

    Jonathas F. R. Lobo

    2012-12-01

    Full Text Available The genus Eremanthus is recognized by the predominance of sesquiterpene lactones from the furanoheliangolide type, a class of substances extensively tested against cancer cell lines. Thus, the species E. crotonoides (DC. Sch. Bip., Asteraceae, obtained on "restinga" vegetation was evaluated against U251 and U87-MG glioma cell lines using the MTT colorimetric assay. Dichloromethane fraction was cytotoxic to both glioblastoma multiforme cell lines. We then conducted UPLC-PDA-ESI-MS/MS analysis of the dichloromethane fraction, which allowed the identification of the sesquiterpene lactones centratherin and goyazensolide. The isolation of centratherin was performed using chromatographic techniques and the identification of this substance was confirmed according to NMR data. Cytotoxic activity of centratherin alone was also evaluated against both U251 and U87-MG cells, which showed IC50 values comparable with those obtained for the commercial anticancer drug doxorubicin. All the tested samples showed cytotoxic activity against glioblastoma multiforme cells which suggests that E. crotonoides extracts may be important sources of antiproliferative substances and that the centratherin may serve as prototype for developing new antiglioblastoma drugs.

  15. Programmed Cell Death During Female Gametophyte Development

    Energy Technology Data Exchange (ETDEWEB)

    Drews, Gary, N.

    2004-09-15

    Endosperm is a storage tissue in the angiosperm seed that is important both biologically and agriculturally. Endosperm is biologically important because it provides nutrients to the embryo during seed development and agriculturally important because it is a significant source of food, feed, and industrial raw materials. Approximately two-thirds of human calories are derived from endosperm, either directly or indirectly through animal feed. Furthermore, endosperm is used as a raw material for numerous industrial products including ethanol. A major event in endosperm development is the transition between the syncytial phase, during which the endosperm nuclei undergo many rounds of mitosis without cytokinesis, and the cellularized phase, during which cell walls form around the endosperm nuclei. Understanding how the syncytial-cellular transition is regulated is agriculturally important because it influences seed size, seed sink strength, and grain weight. However, the molecular processes controlling this transition are not understood. This project led to the identification of the AGL62 gene that regulates the syncytial-cellular transition during endosperm development. AGL62 is expressed during the syncytial phase and suppresses endosperm cellularization during this period. AGL62 most likely does so by suppressing the expression of genes required for cellularization. At the end of the syncytial phase, the FIS PcG complex suppresses AGL62 expression, which allows expression of the cellularization genes and triggers the initiation of the cellularized phase. Endosperm arises following fertilization of the central cell within the female gametophyte. This project also led to the identification of the AGL80 gene that is required for development of the central cell into the endosperm. Within the ovule and seed, AGL80 is expressed exclusively in the central cell and uncellularized endosperm. AGL80 is required for expression of several central cell-expressed genes, including

  16. Programmed cell death: a way of life for plants.

    OpenAIRE

    Greenberg, J T

    1996-01-01

    Cell death in higher plants has been widely observed in predictable patterns throughout development and in response to pathogenic infection. Genetic, biochemical, and morphological evidence suggests that these cell deaths occur as active processes and can be defined formally as examples of programmed cell death (PCD). Intriguingly, plants have at least two types of PCD, an observation that is also true of PCD in animals [Schwartz, L. M., Smith, W.W., Jones, M. E. E. & Osborne, B. A. (1993) Pr...

  17. Programming CD8+ T cells for effective immunotherapy

    OpenAIRE

    Hinrichs, Christian S.; Gattinoni, Luca; Restifo, Nicholas P

    2006-01-01

    The differentiation state of CD8+ T cells has emerged as a crucial determinant of their ability to respond to tumor and infection. Signals from T-cell receptors, co-stimulatory molecules and cytokine receptors direct the differentiation process. These signals ‘program’ sustained and heritable gene expression patterns that govern progressive differentiation and lineage commitment. The epigenetic mechanisms by which T cells are programmed are just beginning to be elucidated. Understanding the m...

  18. Changes in Food Choices of Participants in the Special Diabetes Program for Indians–Diabetes Prevention Demonstration Project, 2006–2010

    OpenAIRE

    Teufel-Shone, Nicolette I; Jiang, Luohua; Beals, Janette; Henderson, William G.; Acton, Kelly J.; Roubideaux, Yvette; Manson, Spero M.

    2015-01-01

    Introduction American Indians/Alaska Natives (AI/ANs) have a disproportionately high rate of type 2 diabetes. Changing food choices plays a key role in preventing diabetes. This study documented changes in the food choices of AI/ANs with diagnosed prediabetes who participated in a diabetes prevention program. Methods The Special Diabetes Program for Indians–Diabetes Prevention Demonstration Project implemented the evidence-based Diabetes Prevention Program (DPP) lifestyle intervention in 36 h...

  19. Manufacture, integration and demonstration of polymer solar cells in a lamp for the Lighting Africa initiative

    DEFF Research Database (Denmark)

    Krebs, Frederik C; Damgaard Nielsen, Torben; Fyenbo, Jan;

    2010-01-01

    Semitransparent flexible polymer solar cells were manufactured in a full roll-to-roll process under ambient conditions. After encapsulation a silver based circuit was printed onto the back side of the polymer solar cell module followed by sheeting and application of discrete components and vias...... mm. A hole with a ring was punched in one corner to enable mechanical fixation or tying. The lamp has two states. In the charging state it has a completely flat outline and will charge the battery when illuminated from either side while the front side illumination is preferable. When used as a lamp...

  20. Solar cells. Danish strategy for research, development, demonstration and distribution. Part 2 - Background paper; Solceller. Dansk strategi for forskning, udvikling, demonstration og udbredelse. Del II - Baggrundsnotat

    Energy Technology Data Exchange (ETDEWEB)

    2009-07-01

    The background paper to the strategy for Danish R and D within the solar cell field describes the background and references for solar cell technology and its development, both nationally and internationally. Especially, the potentials for use of solar cells in Denmark together with the primary Danish actors and strongholds are presented.

  1. In vitro reactivity to implant metals demonstrates a person-dependent association with both T-cell and B-cell activation.

    Science.gov (United States)

    Hallab, Nadim James; Caicedo, Marco; Epstein, Rachel; McAllister, Kyron; Jacobs, Joshua J

    2010-02-01

    Hypersensitivity to metallic implants remains relatively unpredictable and poorly understood. We initially hypothesized that metal-induced lymphocyte proliferation responses to soluble metal challenge (ions) are mediated exclusively by early T-cell activation (not B-cells), typical of a delayed-type-hypersensitivity response. We tested this by comparing proliferation (6 days) of primary lymphocytes with early T-cell and B-cell activation (48 h) in three groups of subjects likely to demonstrate elevated metal reactivity: group 1 (n = 12) history of metal sensitivity with no implant; group 2a (n = 6) well performing metal-on-metal THRs, and group 2b (n = 20) subjects with poorly performing metal-on-polymer total joint arthroplasties (TJA). Group 1 showed 100% (12/12) metal reactivity (stimulation index > 2) to Ni. Groups 2a and 2b were 83% (5/6) and 75% (15/22) metal reactive (to Co, Cr, or Ni), respectively. Of the n = 32 metal-reactive subjects to Co, Cr, or Ni (SI > 2), n = 22/32 demonstrated >2-fold elevations in % of T-cell or B-cell activation (CD25+, CD69+) to metal challenge when compared with untreated control. 18/22 metal-activated subjects demonstrated an exclusively T-cell or B-cell activation response to metal challenge, where 6/18 demonstrated exclusively B-cell activation and 12/18 demonstrated a T-cell only response, as measured by surface activation markers CD25+ and CD69+. However, there was no direct correlation (R(2) metal reactivity than did subject-dependent results of flow-cytometry analysis of T-cell or B-cell activation. The high incidence of lymphocyte reactivity and activation indicate that more complex than initially hypothesized immune responses may contribute to the etiology of debris-induced osteolysis in metal-sensitive individuals.

  2. An introduction on the demonstration performance of fuel cell buses (FCB) in Beijing

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Since the 1990’s, hydrogen has found broad use in the traffic segment. Compared with conventional ones, hydrogen fuelled vehicles, a new generation of clean vehicles, produce no pollutants, with higher energy efficiency. In today’s world where the pollution is tougher, the "Zero Pollution" fuel cell buses display a

  3. Teaching the Toolkit: A Laboratory Series to Demonstrate the Evolutionary Conservation of Metazoan Cell Signaling Pathways

    Science.gov (United States)

    LeClair, Elizabeth E.

    2008-01-01

    A major finding of comparative genomics and developmental genetics is that metazoans share certain conserved, embryonically deployed signaling pathways that instruct cells as to their ultimate fate. Because the DNA encoding these pathways predates the evolutionary split of most animal groups, it should in principle be possible to clone…

  4. Experiences from Swedish demonstration projects with phosphoric acid fuel cells; Erfarenheter fraan svenska demonstrationsprojekt med fosforsyrabraensleceller

    Energy Technology Data Exchange (ETDEWEB)

    Carlsson, Per [Sycon Energikonsult AB, Stockholm (Sweden); Sarkoezi, Laszlo [Vattenfall Utveckling AB, Stockholm (Sweden)

    1999-10-01

    In Sweden, there are today two phosphoric acid fuel cells installed, one PC25A which have been in operation in more than 4 years, and one PC25C which have been in operation for two years. The aim with this project has been two compare operation characteristics, performance, and operation experiences for these two models.

  5. A shift to organismal stress resistance in programmed cell death mutants.

    Directory of Open Access Journals (Sweden)

    Meredith E Judy

    Full Text Available Animals have many ways of protecting themselves against stress; for example, they can induce animal-wide, stress-protective pathways and they can kill damaged cells via apoptosis. We have discovered an unexpected regulatory relationship between these two types of stress responses. We find that C. elegans mutations blocking the normal course of programmed cell death and clearance confer animal-wide resistance to a specific set of environmental stressors; namely, ER, heat and osmotic stress. Remarkably, this pattern of stress resistance is induced by mutations that affect cell death in different ways, including ced-3 (cell death defective mutations, which block programmed cell death, ced-1 and ced-2 mutations, which prevent the engulfment of dying cells, and progranulin (pgrn-1 mutations, which accelerate the clearance of apoptotic cells. Stress resistance conferred by ced and pgrn-1 mutations is not additive and these mutants share altered patterns of gene expression, suggesting that they may act within the same pathway to achieve stress resistance. Together, our findings demonstrate that programmed cell death effectors influence the degree to which C. elegans tolerates environmental stress. While the mechanism is not entirely clear, it is intriguing that animals lacking the ability to efficiently and correctly remove dying cells should switch to a more global animal-wide system of stress resistance.

  6. [Selective "death programs" or pleiotropic"life programs"? Looking for programmed cell death in the light of evolution].

    Science.gov (United States)

    Ameisen, Jean-Claude

    2005-01-01

    "Nothing in biology makes sense except in the light of evolution", wrote Theodosius Dobzhansky, one of the founders of the Modern Synthesis that led to the unification of evolutionary theory and genetics in the midst of the 20th century. Programmed cell death is a genetically regulated process of cell suicide that is central to the development, homeostasis and integrity of multicellular organisms. Conversely, the dysregulation of mechanisms controlling cell suicide plays a role in the pathogenesis of a wide range of diseases. While great progress has been achieved in the unveiling of the molecular mechanisms of programmed cell death, a new, and somehow puzzling level of complexity has recently begun to emerge, suggesting i) that several different self destruction pathways may exist and operate in parallel in our cells, and ii) that molecular effectors of cell suicide might also perform other functions unrelated to cell death induction and crucial to cell survival, such as cell differentiation, metabolism, and the regulation of the cell cycle. These new findings, with important physiopathological and therapeutic implications, seem at odds with the paradigm of programmed cell death derived from the studies of Caenorhabditis elegans, which led to the concept of the existence of selective, bona fide death genes that emerged and became selected for their sole capacity to execute or repress cell death. In this review, I will argue that this new level of complexity might only make sense and be understood when considered in a broader evolutionary context than that of our phylogenetic divergence from C. elegans. A new view of the regulated cell death pathways emerges when one attempts to ask the question of when and how they may have become selected during a timeline of 4 billion years, at the level of ancestral single-celled organisms, including the bacteria. I will argue that there may be no such thing as a bona fide genetic cell death program. Rather, in the framework of

  7. Passivation ability of graphene oxide demonstrated by two-different-metal solar cells

    OpenAIRE

    Hsu, Wen-Tzu; Tsai, Zong-Sian; Chen, Liang-Chun; Chen, Guan-Yu; Lin, Chun-Chieh; Chen, Mei-Hsin; Song, Jenn-Ming; Lin, Chu-Hsuan

    2014-01-01

    The study on graphene oxide (GO) grows rapidly in recent years. We find that graphene oxide could act as the passivation material in photovoltaic applications. Graphene oxide has been applied on Si two-different-metal solar cells. The suitable introduction of graphene oxide could result in obvious enhancement on the efficiency. The simple chemical process to deposit graphene oxide makes low thermal budget, large-area deposition, and fast production of surface passivation possible. The differe...

  8. Heterogeneity of cell-associated CP5 expression on Staphylococcus aureus strains demonstrated by flow cytometry.

    OpenAIRE

    POUTREL, B.; Rainard, P; Sarradin, P.

    1997-01-01

    It was reported previously that two capsular polysaccharides, types 5 and 8 (CP5 and CP8), account for 70 to 80% of Staphylococcus aureus strains isolated from human and animal sources. The capsular material has been shown to play a part in virulence and in resistance to phagocytosis. With a view to investigating the role that CP plays in pathogenicity or protection, relative measurement of cell-associated CP is desirable. Flow cytometry, which permits the analysis of individual bacteria, was...

  9. Office of Technology Development`s Research, Development, Demonstration, Testing and Evaluation Mid-Year Program Review. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This document presents brief summaries of waste management, remedial action, decommissioning/decontamination, and waste processing programs and issues currently being developed at Department of Energy Facilities.

  10. Office of Technology Development's Research, Development, Demonstration, Testing and Evaluation Mid-Year Program Review. Volume 1

    International Nuclear Information System (INIS)

    This document presents brief summaries of waste management, remedial action, decommissioning/decontamination, and waste processing programs and issues currently being developed at Department of Energy Facilities

  11. Synthetic biology: programming cells for biomedical applications.

    Science.gov (United States)

    Hörner, Maximilian; Reischmann, Nadine; Weber, Wilfried

    2012-01-01

    The emerging field of synthetic biology is a novel biological discipline at the interface between traditional biology, chemistry, and engineering sciences. Synthetic biology aims at the rational design of complex synthetic biological devices and systems with desired properties by combining compatible, modular biological parts in a systematic manner. While the first engineered systems were mainly proof-of-principle studies to demonstrate the power of the modular engineering approach of synthetic biology, subsequent systems focus on applications in the health, environmental, and energy sectors. This review describes recent approaches for biomedical applications that were developed along the synthetic biology design hierarchy, at the level of individual parts, of devices, and of complex multicellular systems. It describes how synthetic biological parts can be used for the synthesis of drug-delivery tools, how synthetic biological devices can facilitate the discovery of novel drugs, and how multicellular synthetic ecosystems can give insight into population dynamics of parasites and hosts. These examples demonstrate how this new discipline could contribute to novel solutions in the biopharmaceutical industry. PMID:23502560

  12. Towards sustainable urban transportation: Test, demonstration and development of fuel cell and hybrid-electric buses

    International Nuclear Information System (INIS)

    Several aspects make today's transport system non-sustainable: - Production, transport and combustion of fossil fuels lead to global and local environmental problems. - Oil dependency in the transport sector may lead to economical and political instability. - Air pollution, noise, congestion and land-use may jeopardise public health and quality of life, especially in urban areas. In a sustainable urban transport system most trips are made with public transport because high convenience and comfort makes travelling with public transport attractive. In terms of emissions, including noise, the vehicles are environmentally sustainable, locally as well as globally. Vehicles are energy-efficient and the primary energy stems from renewable sources. Costs are reasonable for all involved, from passengers, bus operators and transport authorities to vehicle manufacturers. The system is thus commercially viable on its own merits. This thesis presents the results from three projects involving different concept buses, all with different powertrains. The first two projects included technical evaluations, including tests, of two different fuel cell buses. The third project focussed on development of a series hybrid-bus with internal combustion engine intended for production around 2010. The research on the fuel cell buses included evaluations of the energy efficiency improvement potential using energy mapping and vehicle simulations. Attitudes to hydrogen fuel cell buses among passengers, bus drivers and bus operators were investigated. Safety aspects of hydrogen as a vehicle fuel were analysed and the use of hydrogen compared to electrical energy storage were also investigated. One main conclusion is that a city bus should be considered as one energy system, because auxiliaries contribute largely to the energy use. Focussing only on the powertrain is not sufficient. The importance of mitigating losses far down an energy conversion chain is emphasised. The Scania hybrid fuel cell

  13. Evaluation and demonstration of methods for improved fuel utilization. Fort Calhoun Poolside Inspection Programs: end-of-cycles 4 and 5

    Energy Technology Data Exchange (ETDEWEB)

    LaVake, J.C.; Smith, G.P.

    1980-08-01

    The purpose of this program is to demonstrate two techniques for improving fuel utilization in current Pressurized Water Reactors (PWR): (1) more efficient fuel management, and (2) high burnup. These improvements are being demonstrated in the Fort Calhoun reactor, a 1420 Mwt PWR. A more efficient fuel management scheme called SAVFUEL (Shimmed And Very Flexible Uranium Element Loading), will be demonstrated which reduces neutron leakage and is expected to reduce uranium requirements. Another part of the demonstration program will increase the fuel burnup of the current 14 x 14 fuel design to further reduce uranium requirements. The program is a joint Department of Energy (DOE), Omaha Public Power District (OPPD) and Combustion Engineering (C-E) endeavor and is part of a national effort to improve uranium utilization in light water reactors.

  14. Evaluation and demonstration of methods for improved fuel utilization. Fort Calhoun Poolside Inspection Programs: end-of-cycles 4 and 5

    International Nuclear Information System (INIS)

    The purpose of this program is to demonstrate two techniques for improving fuel utilization in current Pressurized Water Reactors (PWR): (1) more efficient fuel management, and (2) high burnup. These improvements are being demonstrated in the Fort Calhoun reactor, a 1420 Mwt PWR. A more efficient fuel management scheme called SAVFUEL (Shimmed And Very Flexible Uranium Element Loading), will be demonstrated which reduces neutron leakage and is expected to reduce uranium requirements. Another part of the demonstration program will increase the fuel burnup of the current 14 x 14 fuel design to further reduce uranium requirements. The program is a joint Department of Energy (DOE), Omaha Public Power District (OPPD) and Combustion Engineering (C-E) endeavor and is part of a national effort to improve uranium utilization in light water reactors

  15. Niclosamide, an old antihelminthic agent, demonstrates antitumor activity by blocking multiple signaling pathways of cancer stem cells

    OpenAIRE

    Pan, Jing-Xuan; Ding, Ke; Wang, Cheng-Yan

    2012-01-01

    Niclosamide, an oral antihelminthic drug, has been used to treat tapeworm infection for about 50 years. Niclosamide is also used as a molluscicide for water treatment in schistosomiasis control programs. Recently, several groups have independently discovered that niclosamide is also active against cancer cells, but its precise mechanism of antitumor action is not fully understood. Evidence supports that niclosamide targets multiple signaling pathways (NF-κB, Wnt/β-catenin, Notch, ROS, mTORC1,...

  16. Liquid Tin Anode Direct Coal Fuel Cell Final Program Report

    Energy Technology Data Exchange (ETDEWEB)

    Tao, Thomas

    2012-01-26

    This SBIR program will result in improved LTA cell technology which is the fundamental building block of the Direct Coal ECL concept. As described below, ECL can make enormous efficiency and cost contributions to utility scale coal power. This program will improve LTA cells for small scale power generation. As described in the Commercialization section, there are important intermediate military and commercial markets for LTA generators that will provide an important bridge to the coal power application. The specific technical information from this program relating to YSZ electrolyte durability will be broadly applicable SOFC developers working on coal based SOFC generally. This is an area about which very little is currently known and will be critical for successfully applying fuel cells to coal power generation.

  17. Genetic mutations in accordance with a low malignant potential tumour are not demonstrated in clear cell papillary renal cell carcinoma.

    Science.gov (United States)

    Raspollini, Maria Rosaria; Castiglione, Francesca; Cheng, Liang; Montironi, Rodolfo; Lopez-Beltran, Antonio

    2016-06-01

    Clear cell papillary renal cell carcinoma (CCPRCC) cases were evaluated for mutations on the following genes: KRAS, NRAS, BRAF, PIK3CA, ALK, ERBB2, DDR2, MAP2K1, RET and EGFR. Four male and three female patients of age 42-74 years were evaluated. All cases were incidentally detected by ultrasound and ranged 1.8-3.5 cm. Microscopic examination showed variably tubulopapillary, tubular acinar, cystic architecture and the characteristic linear arrangement of nuclei. The cells were reactive with CK7 (strong), CA IX (cup-shape) and 34 β E12. CD10, AMACR/RACEMASE and GATA3 were negative. There were no mutations on any of the investigated genes. This preliminary observation supports the concept that CCPRCC might be indeed an indolent tumour worth it to be named as clear cell papillary neoplasm of low potential. PMID:26941183

  18. SLUDGE WASHING AND DEMONSTRATION OF THE DWPF FLOWSHEET IN THE SRNL SHIELDED CELLS FOR SLUDGE BATCH 7A QUALIFICATION

    Energy Technology Data Exchange (ETDEWEB)

    Pareizs, J.; Billings, A.; Click, D.

    2011-07-08

    Waste Solidification Engineering (WSE) has requested that characterization and a radioactive demonstration of the next batch of sludge slurry (Sludge Batch 7a*) be completed in the Shielded Cells Facility of the Savannah River National Laboratory (SRNL) via a Technical Task Request (TTR). This characterization and demonstration, or sludge batch qualification process, is required prior to transfer of the sludge from Tank 51 to the Defense Waste Processing Facility (DWPF) feed tank (Tank 40). The current WSE practice is to prepare sludge batches in Tank 51 by transferring sludge from other tanks. Discharges of nuclear materials from H Canyon are often added to Tank 51 during sludge batch preparation. The sludge is washed and transferred to Tank 40, the current DWPF feed tank. Prior to transfer of Tank 51 to Tank 40, SRNL simulates the Tank Farm and DWPF processes with a Tank 51 sample (referred to as the qualification sample). Sludge Batch 7a (SB7a) is composed of portions of Tanks 4, 7, and 12; the Sludge Batch 6 heel in Tank 51; and a plutonium stream from H Canyon. SRNL received the Tank 51 qualification sample (sample ID HTF-51-10-125) following sludge additions to Tank 51. This report documents: (1) The washing (addition of water to dilute the sludge supernate) and concentration (decanting of supernate) of the SB7a - Tank 51 qualification sample to adjust sodium content and weight percent insoluble solids to Tank Farm projections. (2) The performance of a DWPF Chemical Process Cell (CPC) simulation using the washed Tank 51 sample. The simulation included a Sludge Receipt and Adjustment Tank (SRAT) cycle, where acid was added to the sludge to destroy nitrite and reduce mercury, and a Slurry Mix Evaporator (SME) cycle, where glass frit was added to the sludge in preparation for vitrification. The SME cycle also included replication of five canister decontamination additions and concentrations. Processing parameters were based on work with a non

  19. The US Army Foreign Comparative Test fuel cell program

    Science.gov (United States)

    Bostic, Elizabeth; Sifer, Nicholas; Bolton, Christopher; Ritter, Uli; Dubois, Terry

    The US Army RDECOM initiated a Foreign Comparative Test (FCT) Program to acquire lightweight, high-energy dense fuel cell systems from across the globe for evaluation as portable power sources in military applications. Five foreign companies, including NovArs, Smart Fuel Cell, Intelligent Energy, Ballard Power Systems, and Hydrogenics, Inc., were awarded competitive contracts under the RDECOM effort. This paper will report on the status of the program as well as the experimental results obtained from one of the units. The US Army has interests in evaluating and deploying a variety of fuel cell systems, where these systems show added value when compared to current power sources in use. For low-power applications, fuel cells utilizing high-energy dense fuels offer significant weight savings over current battery technologies. This helps reduce the load a solider must carry for longer missions. For high-power applications, the low operating signatures (acoustic and thermal) of fuel cell systems make them ideal power generators in stealth operations. Recent testing has been completed on the Smart Fuel Cell A25 system that was procured through the FCT program. The "A-25" is a direct methanol fuel cell hybrid and was evaluated as a potential candidate for soldier and sensor power applications.

  20. Demonstration of the BNL Continuous Dual Trap Analyzer to Detect Perfluorocarbon Tracers for the Tag, Track and Location Program

    Energy Technology Data Exchange (ETDEWEB)

    Heiser,J.H.; Adams, J.; Dietz, R..; Milian, L.; Watson, T.

    2008-10-07

    The Tag, Track and Location System (TTL) Program is investigating methods of tracking an asset using perfluorocarbon tracers (PFT). The success of any TTL method requires sound detection/location instrumentation. Tracer Detection Technologies Corp (TDT), through a contract with the Office of Naval Research (ONR), is investigating different detection systems. The detections systems generally fall into two categories; proximity detectors and standoff detectors. Proximity detectors, as the name implies, need to be in close proximity (e.g., meter to 10's of meters) to the PFT source. Standoff detection searches for the PFT from a greater distance away from the source (e.g., 100's of meters to kilometers). Gas Chromatographs (GC) are generally considered a proximity detection systems, but in the case of PFTs should be considered for both proximity and standoff detection with the caveat that in standoff use the GC needs to be somewhere in the PFT plume, i.e., generally downwind of the source. With a properly sized PFT source, the right GC can afford fairly large standoff (distance from the source) distances; 100's of meters to kilometers downwind. Brookhaven National Laboratory (BNL) has such a GC system and offered to demonstrate the CDTA for TTL as a no cost addition to the TDTTTL project, of which BNL was a participant. BNL is a leading authority on the sampling, collection, release and detection of PFTs. In addition, the BNL team has extensive background in atmospheric dispersion, the application of PFTs to such studies and the development of applications utilizing PFTs such as building infiltration measurements, control room integrity determination, leak location and environmental investigations. This experience and expertise is essential in developing any PFT application were dispersion, dilution and overcoming environmental conditions and interferences are integral to success. BNL has developed sophisticated gas chromatography methods and

  1. The cause of cancer: biochemical defects in the cancer cell demonstrated by the effects of electromagnetic radiation, glucose and oxygen.

    Science.gov (United States)

    Holt, J A

    1979-01-01

    The responses of a person carrying a cancer to 434 MHz electromagnetic radiation are such that they demonstrate that each human cell contains at least two separate respiratory pathways which can convert glucose to energy. The first pathway produces energy by an anaerobic mechanism and it can be demonstrated that this energy is used for the purpose of cell replication. The second pathway(s) produces energy from glucose by aerobic oxidative processes which can be shown to energise other cellular functions. One of these functions is that of controlling its own cell division. Other demonstrable functions of this aerobic energy producing mechanism are concerned with individual cellular repair processes, multicellular organisation and repair and the preservation of gross anatomical perfection. Release of the first or anaerobic system from supervisory control by the second or aerobic sytem(s) permits the unlimited cell division which is the phenomenon known as cancer. The available circumstantial evidence suggests that this is not the result of nuclear or chromosomal defects or mutations but is due to direct irreversible sublethal damage to the cellular aerobic glucose metabolic system whilst the anaerobic system remains intact. PMID:459964

  2. Single cell analysis demonstrating somatic mosaicism involving 11p in a patient with paternal isodisomy and Beckwith-Wiedemann Syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Bischoff, F.Z.; McCaskill, C.; Subramanian, S. [Baylor College of Medicine, Houston, TX (United States)] [and others

    1994-09-01

    Beckwith-Wiedemann Syndrome (BWS) is characterized by numerous growth abnormalities including exomphalos, macroglossia, gigantism, and hemihypertrophy or hemihyperplasia. The {open_quotes}BWS gene{close_quotes} appears to be maternally repressed and is suspected to function as a growth factor or regulator of somatic growth, since activation of this gene through a variety of mechanisms appears to result in somatic overgrowth and tumor development. Mosaic paternal isodisomy of 11p has been observed previously by others in patients with BWS by Southern blot analysis of genomic DNA. The interpretation of these results was primarily based on the intensities of the hybridization signals for the different alleles. In our study, we demonstrate somatic mosaicism directly through PCR and single cell analysis. Peripheral blood was obtained from a patient with BWS and initial genomic DNA analysis by PCR was suggestive of somatic mosaicism for paternal isodisomy of 11p. Through micromanipulation, single cells were isolated and subjected to primer extention preamplification. Locus-specific microsatellite marker analyses by PCR were performed to determine the chromosome 11 origins in the preamplified individual cells. Two populations of cells were detected, a population of cells with normal biparental inheritance and a population of cells with paternal isodisomy of 11p and biparental disomy of 11q. Using the powerful approach of single cell analysis, the detected somatic mosaicism provides evidence for a mitotic recombinational event that has resulted in loss of the maternal 11p region and gain of a second copy of paternal 11p in some cells. The direct demonstration of mosaicism may explain the variable phenotypes and hemihypertrophy often observed in BWS.

  3. Demonstration of neutron detection utilizing open cell foam and noble gas scintillation

    Energy Technology Data Exchange (ETDEWEB)

    Lavelle, C. M., E-mail: christopher.lavelle@jhuapl.edu; Miller, E. C. [The Johns Hopkins University Applied Physics Laboratory, Asymmetric Operations Department, Laurel, Maryland 20723 (United States); Coplan, M. [Institute for Physical Science and Technology, University of Maryland College Park, Maryland 20142 (United States); Thompson, Alan K.; Vest, Robert E.; Yue, A. T. [National Institute of Standards and Technology, Gaithersburg, Maryland 20899 (United States); Kowler, A. L. [Department of Chemical Physics, University of Maryland, College Park, Maryland 20142 (United States); Koeth, T. [Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, Maryland 20142 (United States); Al-Sheikhly, M. [Department of Materials Science and Engineering, University of Maryland, College Park, Maryland 20742 (United States); Clark, Charles W. [Institute for Physical Science and Technology, University of Maryland College Park, Maryland 20142 (United States); National Institute of Standards and Technology, Gaithersburg, Maryland 20899 (United States); Joint Quantum Institute, National Institute of Standards and Technology and University of Maryland, Gaithersburg, Maryland 20899 (United States)

    2015-03-02

    We present results demonstrating neutron detection via a closely spaced converter structure coupled to low pressure noble gas scintillation instrumented by a single photo-multiplier tube (PMT). The converter is dispersed throughout the gas volume using a reticulated vitreous carbon foam coated with boron carbide (B{sub 4}C). A calibrated cold neutron beam is used to measure the neutron detection properties, using a thin film of enriched {sup 10}B as a reference standard. Monte Carlo computations of the ion energy deposition are discussed, including treatment of the foam random network. Results from this study indicate that the foam shadows a significant portion of the scintillation light from the PMT. The high scintillation yield of Xe appears to overcome the light loss, facilitating neutron detection and presenting interesting opportunities for neutron detector design.

  4. Extensive gastric varices demonstrated by technetium-99m red blood cell scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Domstad, P.A.; Loh, F.G.; Pulmano, C.

    1987-04-01

    An alcohol abuse patient complicated by chronic pancreatitis had splenic vein thrombosis leading to gastric varices and underwent abdominal Tc-99m red blood cell scintigraphy. First pass study, sequential images up to 1 hour, and a 2.5 hour image showed abnormal radioactivity in the left side of the abdomen and midabdomen. In 24 hour images, the high level of activity in the left side persisted; in addition, there was accumulation of radioactivity in the cecum, ascending, transverse colon, the splenic flexure, and descending colon. A splenectomy was performed and during the surgical procedure, a large dilated vein in the greater omentum was noted. It is reemphasized that delayed imaging up to 24 hours is important when the results of earlier images are equivocal or negative.

  5. Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection.

    Science.gov (United States)

    Melo, T C; Araldi, R P; Pessoa, N S D; de-Sá-Júnior, P L; Carvalho, R F; Beçak, W; Stocco, R C

    2015-01-01

    Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive virus infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas and blood were collected from bovines with BPV infection and five samples of peripheral blood and one sample of normal tissue were collected from a calf without BPV infection. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and examined by reverse transcription-polymerase chain reaction, immunofluorescence, in situ hybridization, and electron microscopy. The tissue samples were examined for histopathological and immunohistochemical features. The skin papillomas showed the presence of DNA sequences of BPV-2, BPV-11, and a putative virus type. The blood samples showed DNA sequences of BPV-1, 2, and 4 simultaneously. Immunohistochemistry showed BPV L1 protein in both epithelium and stroma and BPV E2 protein in koilocytes. In situ hybridization confirmed the presence of BPV DNA in PBMCs and immunofluorescence showed nuclear labeling of E2 and L1 BPV proteins in PBMCs. The transcription analysis revealed transcripts of BPV-1 L1, BPV-2 L2, and BPV-4 E7 in blood and papilloma samples of BPV-infected cattle. The comet assay revealed high levels of host cell DNA damage upon BPV infection. Electron microscopy analysis of PBMCs identified the presence of particles in the cytoplasm that are consistent with papillomavirus in size and shape. The productive infection of PBMCs with BPV has been previously discussed and this study provides evidence indicating that PBMCs are a target of BPV. PMID:26681018

  6. Control of T cell antigen reactivity via programmed TCR downregulation.

    Science.gov (United States)

    Gallegos, Alena M; Xiong, Huizhong; Leiner, Ingrid M; Sušac, Bože; Glickman, Michael S; Pamer, Eric G; van Heijst, Jeroen W J

    2016-04-01

    The T cell antigen receptor (TCR) is unique in that its affinity for ligand is unknown before encounter and can vary by orders of magnitude. How the immune system regulates individual T cells that display very different reactivity to antigen remains unclear. Here we found that activated CD4(+) T cells, at the peak of clonal expansion, persistently downregulated their TCR expression in proportion to the strength of the initial antigen recognition. This programmed response increased the threshold for cytokine production and recall proliferation in a clone-specific manner and ultimately excluded clones with the highest antigen reactivity. Thus, programmed downregulation of TCR expression represents a negative feedback mechanism for constraining T cell effector function with a suitable time delay to thereby allow pathogen control while avoiding excess inflammatory damage. PMID:26901151

  7. Current status of Westinghouse tubular solid oxide fuel cell program

    Energy Technology Data Exchange (ETDEWEB)

    Parker, W.G. [Westinghouse Science and Technology Center, Pittsburgh, PA (United States)

    1996-04-01

    In the last ten years the solid oxide fuel cell (SOFC) development program at Westinghouse has evolved from a focus on basic material science to the engineering of fully integrated electric power systems. Our endurance for this cell is 5 to 10 years. To date we have successfully operated at power for over six years. For power plants it is our goal to have operated before the end of this decade a MW class power plant. Progress toward these goals is described.

  8. Office of Technology Development's Research, Development, Demonstration, Testing and Evaluation Mid-Year Program Review. Volume 2

    International Nuclear Information System (INIS)

    This document, Volume 2, presents brief summaries of programs being investigated at USDOE sites for waste processing, remedial action, underground storage tank remediation, and robotic applications in waste management

  9. Office of Technology Development`s Research, Development, Demonstration, Testing and Evaluation Mid-Year Program Review. Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This document, Volume 2, presents brief summaries of programs being investigated at USDOE sites for waste processing, remedial action, underground storage tank remediation, and robotic applications in waste management.

  10. A Recently Established Murine Model of Nasal Polyps Demonstrates Activation of B Cells, as Occurs in Human Nasal Polyps.

    Science.gov (United States)

    Kim, Dong-Young; Lee, Sun Hye; Carter, Roderick G; Kato, Atsushi; Schleimer, Robert P; Cho, Seong H

    2016-08-01

    Animal model systems are invaluable for examining human diseases. Our laboratory recently established a mouse model of nasal polyps (NPs) and investigated similarities and differences between this mouse model and human NPs. We especially focus on the hypothesis that B cell activation occurs during NP generation in the murine model. After induction of ovalbumin-induced allergic rhinosinusitis, 6% ovalbumin and Staphylococcus aureus enterotoxin B (10 ng) were instilled into the nasal cavity of mice three times per week for 8 weeks. The development of structures that somewhat resemble NPs (which we will refer to as NPs) was confirmed by hematoxylin and eosin staining. The mRNA and protein levels of various inflammatory cell markers and mediators were measured by real-time PCR in nasal tissue and by ELISA in nasal lavage fluid (NLF), respectively. Total Ig isotype levels in NLF were also quantitated using the Mouse Ig Isotyping Multiplex kit (EMD Millipore, Billerica, MA) on a Luminex 200 instrument (Life Technologies, Grand Island, NY). Similar to human NPs, there were significant increases in gene expression of inflammatory cell markers, such as CD19, CD138, CD11c, and mast cell protease-6 in nasal tissue samples of the NP group compared with those of the control group. In further investigations of B cell activation, mRNA expressions of B cell activating factor and a proliferation-inducing ligand were found to be significantly increased in mouse NP tissue. B cell-activating factor protein concentration and IgA and IgG1 levels in NLF were significantly higher in the NP group compared with the control group. In this study, the NP mouse model demonstrated enhanced B cell responses, which are reminiscent of B cell responses in human NPs. PMID:27163839

  11. Workshop on programming beta cell development, impairment and regeneration

    DEFF Research Database (Denmark)

    Heller, Scott; Nielsen, Jens Høiriis

    2012-01-01

    Helsingør, the city of Hamlet in Denmark, provided the site for the workshop "Programming Beta Cell Development, Impairment and Regeneration" on October 23-26th, 2011. The same location has held two EASD Islet study group meetings, while the previous three workshops were held in Helsinki, Finland...

  12. Application of laser-accelerated protons to the demonstration of DNA double-strand breaks in human cancer cells

    Science.gov (United States)

    Yogo, A.; Sato, K.; Nishikino, M.; Mori, M.; Teshima, T.; Numasaki, H.; Murakami, M.; Demizu, Y.; Akagi, S.; Nagayama, S.; Ogura, K.; Sagisaka, A.; Orimo, S.; Nishiuchi, M.; Pirozhkov, A. S.; Ikegami, M.; Tampo, M.; Sakaki, H.; Suzuki, M.; Daito, I.; Oishi, Y.; Sugiyama, H.; Kiriyama, H.; Okada, H.; Kanazawa, S.; Kondo, S.; Shimomura, T.; Nakai, Y.; Tanoue, M.; Sasao, H.; Wakai, D.; Bolton, P. R.; Daido, H.

    2009-05-01

    We report the demonstrated irradiation effect of laser-accelerated protons on human cancer cells. In vitro (living) A549 cells are irradiated with quasimonoenergetic proton bunches of 0.8-2.4 MeV with a single bunch duration of 15 ns. Irradiation with the proton dose of 20 Gy results in a distinct formation of γ-H2AX foci as an indicator of DNA double-strand breaks generated in the cancer cells. This is a pioneering result that points to future investigations of the radiobiological effects of laser-driven ion beams. Unique high-current and short-bunch features make laser-driven proton bunches an excitation source for time-resolved determination of radical yields.

  13. Treatment and disposal of radioactive wastes from nuclear power plants. Program for encapsulation, deep geologic deposition and research, development and demonstration; Kaernkraftavfallets behandling och slutfoervaring. Program foer inkapsling, geologisk djupfoervaring samt forskning, utveckling och demonstration

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    Programs for RD and D concerning disposal of radioactive waste are presented. Main topics include: Design, testing and manufacture of canisters for the spent fuels; Design of equipment for deposition of waste canisters; Material and process for backfilling rock caverns; Evaluation of accuracy and validation of methods for safety analyses; Development of methods for defining scenarios for the safety analyses. 471 refs, 67 figs, 21 tabs.

  14. Concise review: programming human pluripotent stem cells into blood.

    Science.gov (United States)

    Easterbrook, Jennifer; Fidanza, Antonella; Forrester, Lesley M

    2016-06-01

    Blood disorders are treated with cell therapies including haematopoietic stem cell (HSC) transplantation as well as platelet and red blood cell transfusions. However the source of cells is entirely dependent on donors, procedures are susceptible to transfusion-transmitted infections and serious complications can arise in recipients due to immunological incompatibility. These problems could be alleviated if it was possible to produce haematopoietic cells in vitro from an autologous and renewable cell source. The production of haematopoietic cells in the laboratory from human induced pluripotent stem cells (iPSCs) may provide a route to realize this goal but it has proven challenging to generate long-term reconstituting HSCs. To date, the optimization of differentiation protocols has mostly relied on the manipulation of extrinsic signals to mimic the in vivo environment. We review studies that have taken an alternative approach to modulate intrinsic signals by enforced expression of transcription factors. Single and combinations of multiple transcription factors have been used in a variety of contexts to enhance the production of haematopoietic cells from human pluripotent stem cells. This programming approach, together with the recent advances in the production and use of synthetic transcription factors, holds great promise for the production of fully functional HSCs in the future. PMID:26996518

  15. Concise review: programming human pluripotent stem cells into blood.

    Science.gov (United States)

    Easterbrook, Jennifer; Fidanza, Antonella; Forrester, Lesley M

    2016-06-01

    Blood disorders are treated with cell therapies including haematopoietic stem cell (HSC) transplantation as well as platelet and red blood cell transfusions. However the source of cells is entirely dependent on donors, procedures are susceptible to transfusion-transmitted infections and serious complications can arise in recipients due to immunological incompatibility. These problems could be alleviated if it was possible to produce haematopoietic cells in vitro from an autologous and renewable cell source. The production of haematopoietic cells in the laboratory from human induced pluripotent stem cells (iPSCs) may provide a route to realize this goal but it has proven challenging to generate long-term reconstituting HSCs. To date, the optimization of differentiation protocols has mostly relied on the manipulation of extrinsic signals to mimic the in vivo environment. We review studies that have taken an alternative approach to modulate intrinsic signals by enforced expression of transcription factors. Single and combinations of multiple transcription factors have been used in a variety of contexts to enhance the production of haematopoietic cells from human pluripotent stem cells. This programming approach, together with the recent advances in the production and use of synthetic transcription factors, holds great promise for the production of fully functional HSCs in the future.

  16. SOLID STATE ENERGY CONVERSION ALLIANCE (SECA) SOLID OXIDE FUEL CELL PROGRAM

    Energy Technology Data Exchange (ETDEWEB)

    Unknown

    2003-06-01

    This report summarizes the progress made during the September 2001-March 2002 reporting period under Cooperative Agreement DE-FC26-01NT41245 for the U. S. Department of Energy, National Energy Technology Laboratory (DOE/NETL) entitled ''Solid State Energy Conversion Alliance (SECA) Solid Oxide Fuel Cell Program''. The program focuses on the development of a low-cost, high-performance 3-to-10-kW solid oxide fuel cell (SOFC) system suitable for a broad spectrum of power-generation applications. The overall objective of the program is to demonstrate a modular SOFC system that can be configured to create highly efficient, cost-competitive, and environmentally benign power plants tailored to specific markets. When fully developed, the system will meet the efficiency, performance, life, and cost goals for future commercial power plants.

  17. Vehicle Technologies and Fuel Cell Technologies Program: Prospective Benefits Assessment Report for Fiscal Year 2016

    Energy Technology Data Exchange (ETDEWEB)

    Stephens, T. S. [Argonne National Lab. (ANL), Argonne, IL (United States); Taylor, C. H. [TA Engineering, Inc., Catonsville, MD (United States); Moore, J. S. [TA Engineering, Inc., Catonsville, MD (United States); Ward, J. [United States Department of Energy, Washington, DC (United States). Office of Energy Efficiency and Renewable Energy

    2016-02-23

    Under a diverse set of programs, the Vehicle Technologies and Fuel Cell Technologies offices of DOE’s Office of Energy Efficiency and Renewable Energy invest in research, development, demonstration, and deployment of advanced vehicle, hydrogen production, delivery and storage, and fuel cell technologies. This report estimates the benefits of successfully developing and deploying these technologies (a “Program Success” case) relative to a base case (the “No Program” case). The Program Success case represents the future with completely successful deployment of Vehicle Technologies Office (VTO) and Fuel Cell Technologies Office (FCTO) technologies. The No Program case represents a future in which there is no contribution after FY 2016 by the VTO or FCTO to these technologies. The benefits of advanced vehicle, hydrogen production, delivery and storage, and fuel cell technologies were estimated on the basis of differences in fuel use, primary energy use, and greenhouse gas (GHG) emissions from light-, medium- and heavy-duty vehicles, including energy and emissions from fuel production, between the base case and the Program Success case. Improvements in fuel economy of various vehicle types, growth in the stock of fuel cell vehicles and other advanced technology vehicles, and decreased GHG intensity of hydrogen production and delivery in the Program Success case over the No Program case were projected to result in savings in petroleum use and GHG emissions. Benefits were disaggregated by individual program technology areas, which included the FCTO program and the VTO subprograms of batteries and electric drives; advanced combustion engines; fuels and lubricants; materials (for reduction in vehicle mass, or “lightweighting”); and, for medium- and heavy-duty vehicles, reduction in rolling and aerodynamic resistance. Projections for the Program Success case indicate that by 2035, the average fuel economy of on-road, light-duty vehicle stock could be 47% to 76

  18. An application programming interface for CellNetAnalyzer.

    Science.gov (United States)

    Klamt, Steffen; von Kamp, Axel

    2011-08-01

    CellNetAnalyzer (CNA) is a MATLAB toolbox providing computational methods for studying structure and function of metabolic and cellular signaling networks. In order to allow non-experts to use these methods easily, CNA provides GUI-based interactive network maps as a means of parameter input and result visualization. However, with the availability of high-throughput data, there is a need to make CNA's functionality also accessible in batch mode for automatic data processing. Furthermore, as some algorithms of CNA are of general relevance for network analysis it would be desirable if they could be called as sub-routines by other applications. For this purpose, we developed an API (application programming interface) for CNA allowing users (i) to access the content of network models in CNA, (ii) to use CNA's network analysis capabilities independent of the GUI, and (iii) to interact with the GUI to facilitate the development of graphical plugins. Here we describe the organization of network projects in CNA and the application of the new API functions to these projects. This includes the creation of network projects from scratch, loading and saving of projects and scenarios, and the application of the actual analysis methods. Furthermore, API functions for the import/export of metabolic models in SBML format and for accessing the GUI are described. Lastly, two example applications demonstrate the use and versatile applicability of CNA's API. CNA is freely available for academic use and can be downloaded from http://www.mpi-magdeburg.mpg.de/projects/cna/cna.html.

  19. An application programming interface for CellNetAnalyzer.

    Science.gov (United States)

    Klamt, Steffen; von Kamp, Axel

    2011-08-01

    CellNetAnalyzer (CNA) is a MATLAB toolbox providing computational methods for studying structure and function of metabolic and cellular signaling networks. In order to allow non-experts to use these methods easily, CNA provides GUI-based interactive network maps as a means of parameter input and result visualization. However, with the availability of high-throughput data, there is a need to make CNA's functionality also accessible in batch mode for automatic data processing. Furthermore, as some algorithms of CNA are of general relevance for network analysis it would be desirable if they could be called as sub-routines by other applications. For this purpose, we developed an API (application programming interface) for CNA allowing users (i) to access the content of network models in CNA, (ii) to use CNA's network analysis capabilities independent of the GUI, and (iii) to interact with the GUI to facilitate the development of graphical plugins. Here we describe the organization of network projects in CNA and the application of the new API functions to these projects. This includes the creation of network projects from scratch, loading and saving of projects and scenarios, and the application of the actual analysis methods. Furthermore, API functions for the import/export of metabolic models in SBML format and for accessing the GUI are described. Lastly, two example applications demonstrate the use and versatile applicability of CNA's API. CNA is freely available for academic use and can be downloaded from http://www.mpi-magdeburg.mpg.de/projects/cna/cna.html. PMID:21315797

  20. Erythropoietin retards DNA breakdown and prevents programmed death in erythroid progenitor cells

    Energy Technology Data Exchange (ETDEWEB)

    Koury, M.J.; Bondurant, M.C. (Vanderbilt Univ. Medical Center, Nashville, TN (USA) Veterans Administration Medical Center, Nashville, TN (USA))

    1990-04-20

    The mechanism by which erythropoietin controls mammalian erythrocyte production is unknown. Labeling experiments in vitro with ({sup 3}H) thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. Thus, apoptosis is a major component of normal erythropoiesis, and erythropoietin controls erythrocyte production by retarding DNA breakdown and preventing apoptosis in erythroid progenitor cells.

  1. Maturing of SOFC cell and stack production technology and preparation for demonstration of SOFC stacks. Part 2

    Energy Technology Data Exchange (ETDEWEB)

    2006-07-01

    The TOFC/Riso pilot plant production facility for the manufacture of anode-supported cells has been further up-scaled with an automated continuous spraying process and an extra sintering capacity resulting in production capacity exceeding 15,000 standard cells (12x12 cm2) in 2006 with a success rate of about 85% in the cell production. All processing steps such as tape-casting, spraying, screen-printing and atmospheric air sintering in the cell production have been selected on condition that up-scaling and cost effective, flexible, industrial mass production are feasible. The standard cell size is currently being increased to 18x18 cm2, and 150 cells of this size have been produced in 2006 for our further stack development. To improve quality and lower production cost, a new screen printing line is under establishment. TOFC's stack design is an ultra compact multilayer assembly of cells (including contact layers), metallic interconnects, spacer frames and glass seals. The compactness ensures minimized material consumption and low cost. Standard stacks with cross flow configuration contains 75 cells (12x12cm2) delivering about 1.2 kW at optimal operation conditions with pre-reformed NG as fuel. Stable performance has been demonstrated for 500-1000 hours. Significantly improved materials, especially concerning the metallic interconnect and the coatings have been introduced during the last year. Small stacks (5-10 cells) exhibit no detectable stack degradation using our latest cells and stack materials during test periods of 500-1000 hours. Larger stacks (50-75 cells) suffer from mal-distribution of gas and air inside the stacks, gas leakage, gas cross-over, pressure drop, and a certain loss of internal electrical contact during operation cycles. Measures have been taken to find solutions during the following development work. The stack production facilities have been improved and up-scaled. In 2006, 5 standard stacks have been assembled and burned in based on

  2. Human induced pluripotent cells resemble embryonic stem cells demonstrating enhanced levels of DNA repair and efficacy of nonhomologous end-joining

    Energy Technology Data Exchange (ETDEWEB)

    Fan Jinshui; Robert, Carine [Department of Radiation Oncology, University of Maryland School of Medicine, 655 West Baltimore Street, BRB 7-023A, Baltimore, MD 21201 (United States); Jang, Yoon-Young; Liu Hua; Sharkis, Saul; Baylin, Stephen Bruce [Johns Hopkins University School of Medicine, Department of Oncology, Baltimore, MD 21231-1000 (United States); Rassool, Feyruz Virgilia, E-mail: frassool@som.umaryland.edu [Department of Radiation Oncology, University of Maryland School of Medicine, 655 West Baltimore Street, BRB 7-023A, Baltimore, MD 21201 (United States)

    2011-08-01

    Highlights: {yields} iPSC and hESC demonstrate a similar cell cycle profile, with increased S phase cells and decreased G0/G1. {yields} iPSC and hESC increased ROS and decreased DSBs, compared with differentiated parental cells. {yields} iPSC and hESC demonstrate elevated DSB repair activity, including nonhomologous end-joining, compared with differentiated parental cells. {yields} iPSC however show a partial apoptotic response to DNA damage, compared to hESC. {yields} DNA damage responses may constitute important markers for the efficacy of iPSC reprogramming. - Abstract: To maintain the integrity of the organism, embryonic stem cells (ESC) need to maintain their genomic integrity in response to DNA damage. DNA double strand breaks (DSBs) are one of the most lethal forms of DNA damage and can have disastrous consequences if not repaired correctly, leading to cell death, genomic instability and cancer. How human ESC (hESC) maintain genomic integrity in response to agents that cause DSBs is relatively unclear. Adult somatic cells can be induced to 'dedifferentiate' into induced pluripotent stem cells (iPSC) and reprogram into cells of all three germ layers. Whether iPSC have reprogrammed the DNA damage response is a critical question in regenerative medicine. Here, we show that hESC demonstrate high levels of endogenous reactive oxygen species (ROS) which can contribute to DNA damage and may arise from high levels of metabolic activity. To potentially counter genomic instability caused by DNA damage, we find that hESC employ two strategies: First, these cells have enhanced levels of DNA repair proteins, including those involved in repair of DSBs, and they demonstrate elevated nonhomologous end-joining (NHEJ) activity and repair efficacy, one of the main pathways for repairing DSBs. Second, they are hypersensitive to DNA damaging agents, as evidenced by a high level of apoptosis upon irradiation. Importantly, iPSC, unlike the parent cells they are derived

  3. Demonstration and Research Pest Control: A Training Program for the Certification of Pesticide Applicators, Manual No. 10.

    Science.gov (United States)

    Rust, Harry K.; And Others

    This manual is intended to provide the information necessary to meet EPA standards for demonstration and research pest control and prepare for the written examination required for certification. Emphasis is placed on the principles of safe pesticide use. Chapters are included on pesticide applicator certification in Virginia, basics of pest…

  4. Generation of cardiac pacemaker cells by programming and differentiation.

    Science.gov (United States)

    Husse, Britta; Franz, Wolfgang-Michael

    2016-07-01

    A number of diseases are caused by faulty function of the cardiac pacemaker and described as "sick sinus syndrome". The medical treatment of sick sinus syndrome with electrical pacemaker implants in the diseased heart includes risks. These problems may be overcome via "biological pacemaker" derived from different adult cardiac cells or pluripotent stem cells. The generation of cardiac pacemaker cells requires the understanding of the pacing automaticity. Two characteristic phenomena the "membrane-clock" and the "Ca(2+)-clock" are responsible for the modulation of the pacemaker activity. Processes in the "membrane-clock" generating the spontaneous pacemaker firing are based on the voltage-sensitive membrane ion channel activity starting with slow diastolic depolarization and discharging in the action potential. The influence of the intracellular Ca(2+) modulating the pacemaker activity is characterized by the "Ca(2+)-clock". The generation of pacemaker cells started with the reprogramming of adult cardiac cells by targeted induction of one pacemaker function like HCN1-4 overexpression and enclosed in an activation of single pacemaker specific transcription factors. Reprogramming of adult cardiac cells with the transcription factor Tbx18 created cardiac cells with characteristic features of cardiac pacemaker cells. Another key transcription factor is Tbx3 specifically expressed in the cardiac conduction system including the sinoatrial node and sufficient for the induction of the cardiac pacemaker gene program. For a successful cell therapeutic practice, the generated cells should have all regulating mechanisms of cardiac pacemaker cells. Otherwise, the generated pacemaker cells serve only as investigating model for the fundamental research or as drug testing model for new antiarrhythmics. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.

  5. Generation of cardiac pacemaker cells by programming and differentiation.

    Science.gov (United States)

    Husse, Britta; Franz, Wolfgang-Michael

    2016-07-01

    A number of diseases are caused by faulty function of the cardiac pacemaker and described as "sick sinus syndrome". The medical treatment of sick sinus syndrome with electrical pacemaker implants in the diseased heart includes risks. These problems may be overcome via "biological pacemaker" derived from different adult cardiac cells or pluripotent stem cells. The generation of cardiac pacemaker cells requires the understanding of the pacing automaticity. Two characteristic phenomena the "membrane-clock" and the "Ca(2+)-clock" are responsible for the modulation of the pacemaker activity. Processes in the "membrane-clock" generating the spontaneous pacemaker firing are based on the voltage-sensitive membrane ion channel activity starting with slow diastolic depolarization and discharging in the action potential. The influence of the intracellular Ca(2+) modulating the pacemaker activity is characterized by the "Ca(2+)-clock". The generation of pacemaker cells started with the reprogramming of adult cardiac cells by targeted induction of one pacemaker function like HCN1-4 overexpression and enclosed in an activation of single pacemaker specific transcription factors. Reprogramming of adult cardiac cells with the transcription factor Tbx18 created cardiac cells with characteristic features of cardiac pacemaker cells. Another key transcription factor is Tbx3 specifically expressed in the cardiac conduction system including the sinoatrial node and sufficient for the induction of the cardiac pacemaker gene program. For a successful cell therapeutic practice, the generated cells should have all regulating mechanisms of cardiac pacemaker cells. Otherwise, the generated pacemaker cells serve only as investigating model for the fundamental research or as drug testing model for new antiarrhythmics. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel

  6. NASA Bioreactor Demonstration System

    Science.gov (United States)

    2002-01-01

    Leland W. K. Chung (left), Director, Molecular Urology Therapeutics Program at the Winship Cancer Institute at Emory University, is principal investigator for the NASA bioreactor demonstration system (BDS-05). With him is Dr. Jun Shu, an assistant professor of Orthopedics Surgery from Kuming Medical University China. The NASA Bioreactor provides a low turbulence culture environment which promotes the formation of large, three-dimensional cell clusters. Due to their high level of cellular organization and specialization, samples constructed in the bioreactor more closely resemble the original tumor or tissue found in the body. The Bioreactor is rotated to provide gentle mixing of fresh and spent nutrient without inducing shear forces that would damage the cells. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: Emory University.

  7. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1979-01-01

    Presents two demonstrations which are intended for chemistry college students. These demonstrations are: (1) enhancement of concentration quenching by micelles; and (2) the thermite lecture demonstration. (HM)

  8. Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens

    Directory of Open Access Journals (Sweden)

    Takami Matsuyama

    2012-05-01

    Full Text Available Immunohistochemistry (IHC for detecting key signal molecules involved in programmed cell death (PCD in archival human pathology specimens is fairly well established. Detection of cleaved caspase-3 in lymphocytes in rheumatoid arthritis (RA and gastric surface foveolar glandular epithelia but not in synoviocytes in RA, gastric fundic glandular epithelia, or nasal NK/T-cell lymphoma (NKTCL cells suggests anti-apoptotic mechanisms in cell differentiation and in oncogenesis such as the induction of survivin. Enzymatically pretreated and ultra-super sensitive detection of beclin-1 in synoviocytes in RA and gastric fundic glandular epithelia suggests enhanced autophagy. The deposition of beclin-1 in fibrinoid necrosis in RA and expression of beclin-1 in detached gastric fundic glandular cells suggest that enhanced autophagy undergoes autophagic cell death (ACD. NKTCL exhibited enhanced autophagy through LC3 labeling and showed densely LC3 labeled cell-debris in regions of peculiar necrosis without deposition of beclin-1, indicating massive ACD in NKTCL and the alternative pathway enhancing autophagy following autophagic vesicle nucleation. Autophagy progression was monitored by labeling aggregated mitochondria and cathepsin D. The cell-debris in massive ACD in NKTCL were positive for 8-hydroxydeoxyguanosine, suggesting DNA oxidation occurred in ACD. Immunohistochemical autophagy and PCD analysis in archival human pathology specimens may offer new insights into autophagy in humans.

  9. Developmental cell death programs license cytotoxic cells to eliminate histocompatible partners.

    Science.gov (United States)

    Corey, Daniel M; Rosental, Benyamin; Kowarsky, Mark; Sinha, Rahul; Ishizuka, Katherine J; Palmeri, Karla J; Quake, Stephen R; Voskoboynik, Ayelet; Weissman, Irving L

    2016-06-01

    In a primitive chordate model of natural chimerism, one chimeric partner is often eliminated in a process of allogeneic resorption. Here, we identify the cellular framework underlying loss of tolerance to one partner within a natural Botryllus schlosseri chimera. We show that the principal cell type mediating chimeric partner elimination is a cytotoxic morula cell (MC). Proinflammatory, developmental cell death programs render MCs cytotoxic and, in collaboration with activated phagocytes, eliminate chimeric partners during the "takeover" phase of blastogenic development. Among these genes, the proinflammatory cytokine IL-17 enhances cytotoxicity in allorecognition assays. Cellular transfer of FACS-purified MCs from allogeneic donors into recipients shows that the resorption response can be adoptively acquired. Transfer of 1 × 10(5) allogeneic MCs eliminated 33 of 78 (42%) recipient primary buds and 20 of 76 (20.5%) adult parental adult organisms (zooids) by 14 d whereas transfer of allogeneic cell populations lacking MCs had only minimal effects on recipient colonies. Furthermore, reactivity of transferred cells coincided with the onset of developmental-regulated cell death programs and disproportionately affected developing tissues within a chimera. Among chimeric partner "losers," severe developmental defects were observed in asexually propagating tissues, reflecting a pathologic switch in gene expression in developmental programs. These studies provide evidence that elimination of one partner in a chimera is an immune cell-based rejection that operates within histocompatible pairs and that maximal allogeneic responses involve the coordination of both phagocytic programs and the "arming" of cytotoxic cells. PMID:27217570

  10. Development and Demonstration of a New Generation High Efficiency 10kW Stationary Fuel Cell System

    Energy Technology Data Exchange (ETDEWEB)

    Howell, Thomas Russell

    2013-04-30

    The overall project objective is to develop and demonstrate a polymer electrolyte membrane fuel cell combined heat and power (PEMFC CHP) system that provides the foundation for commercial, mass produced units which achieve over 40% electrical efficiency (fuel to electric conversion) from 50-100% load, greater than 70% overall efficiency (fuel to electric energy + usable waste heat energy conversion), have the potential to achieve 40,000 hours durability on all major process components, and can be produced in high volumes at under $400/kW (revised to $750/kW per 2011 DOE estimates) capital cost.

  11. Light Water Reactor Sustainability Program Risk Informed Safety Margin Characterization (RISMC) Advanced Test Reactor Demonstration Case Study

    Energy Technology Data Exchange (ETDEWEB)

    Curtis Smith; David Schwieder; Cherie Phelan; Anh Bui; Paul Bayless

    2012-08-01

    Safety is central to the design, licensing, operation, and economics of Nuclear Power Plants (NPPs). Consequently, the ability to better characterize and quantify safety margin holds the key to improved decision making about LWR design, operation, and plant life extension. A systematic approach to characterization of safety margins and the subsequent margins management options represents a vital input to the licensee and regulatory analysis and decision making that will be involved. The purpose of the RISMC Pathway R&D is to support plant decisions for risk-informed margins management with the aim to improve economics, reliability, and sustain safety of current NPPs. Goals of the RISMC Pathway are twofold: (1) Develop and demonstrate a risk-assessment method coupled to safety margin quantification that can be used by NPP decision makers as part of their margin recovery strategies. (2) Create an advanced “RISMC toolkit” that enables more accurate representation of NPP safety margin. This report describes the RISMC methodology demonstration where the Advanced Test Reactor (ATR) was used as a test-bed for purposes of determining safety margins. As part of the demonstration, we describe how both the thermal-hydraulics and probabilistic safety calculations are integrated and used to quantify margin management strategies.

  12. Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death.

    Directory of Open Access Journals (Sweden)

    Takashi Kadono

    Full Text Available BACKGROUND: Ozone is a major secondary air pollutant often reaching high concentrations in urban areas under strong daylight, high temperature and stagnant high-pressure systems. Ozone in the troposphere is a pollutant that is harmful to the plant. PRINCIPAL FINDINGS: By exposing cells to a strong pulse of ozonized air, an acute cell death was observed in suspension cells of Arabidopsis thaliana used as a model. We demonstrated that O(3 treatment induced the activation of a plasma membrane anion channel that is an early prerequisite of O(3-induced cell death in A. thaliana. Our data further suggest interplay of anion channel activation with well known plant responses to O(3, Ca(2+ influx and NADPH-oxidase generated reactive oxygen species (ROS in mediating the oxidative cell death. This interplay might be fuelled by several mechanisms in addition to the direct ROS generation by O(3; namely, H(2O(2 generation by salicylic and abscisic acids. Anion channel activation was also shown to promote the accumulation of transcripts encoding vacuolar processing enzymes, a family of proteases previously reported to contribute to the disruption of vacuole integrity observed during programmed cell death. SIGNIFICANCE: Collectively, our data indicate that anion efflux is an early key component of morphological and biochemical events leading to O(3-induced programmed cell death. Because ion channels and more specifically anion channels assume a crucial position in cells, an understanding about the underlying role(s for ion channels in the signalling pathway leading to programmed cell death is a subject that warrants future investigation.

  13. In vivo detection and imaging of phosphatidylserine expression during programmed cell death

    Science.gov (United States)

    Blankenberg, Francis G.; Katsikis, Peter D.; Tait, Jonathan F.; Davis, R. Eric; Naumovski, Louis; Ohtsuki, Katsuichi; Kopiwoda, Susan; Abrams, Michael J.; Darkes, Marilyn; Robbins, Robert C.; Maecker, Holden T.; Strauss, H.W.

    1998-01-01

    One of the earliest events in programmed cell death is the externalization of phosphatidylserine, a membrane phospholipid normally restricted to the inner leaflet of the lipid bilayer. Annexin V, an endogenous human protein with a high affinity for membrane bound phosphatidylserine, can be used in vitro to detect apoptosis before other well described morphologic or nuclear changes associated with programmed cell death. We tested the ability of exogenously administered radiolabeled annexin V to concentrate at sites of apoptotic cell death in vivo. After derivatization with hydrazinonicotinamide, annexin V was radiolabeled with technetium 99m. In vivo localization of technetium 99m hydrazinonicotinamide-annexin V was tested in three models: fuminant hepatic apoptosis induced by anti-Fas antibody injection in BALB/c mice; acute rejection in ACI rats with transplanted heterotopic PVG cardiac allografts; and cyclophosphamide treatment of transplanted 38C13 murine B cell lymphomas. External radionuclide imaging showed a two- to sixfold increase in the uptake of radiolabeled annexin V at sites of apoptosis in all three models. Immunohistochemical staining of cardiac allografts for exogenously administered annexin V revealed intense staining of numerous myocytes at the periphery of mononuclear infiltrates of which only a few demonstrated positive apoptotic nuclei by the terminal deoxynucleotidyltransferase-mediated UTP end labeling method. These results suggest that radiolabeled annexin V can be used in vivo as a noninvasive means to detect and serially image tissues and organs undergoing programmed cell death. PMID:9600968

  14. Lithium-ion Battery Demonstration for the 2007 NASA Desert Research and Technology Studies (Desert RATS) Program

    Science.gov (United States)

    Bennett, William; Baldwin, Richard

    2007-01-01

    The NASA Glenn Research Center (GRC) Electrochemistry Branch designed and produced five lithium-ion battery packs for demonstration in a portable life support system (PLSS) on spacesuit simulators. The experimental batteries incorporated advanced, NASA-developed electrolytes and included internal protection against over-current, over-discharge and over-temperature. The 500-gram batteries were designed to deliver a constant power of 38 watts over 103 minutes of discharge time (130 Wh/kg). Battery design details are described and field and laboratory test results are summarized.

  15. Office of Technology Development FY 1993 program summary: Office of Research and Development, Office of Demonstration, Testing and Evaluation. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-02-01

    This report summarizes significant FY93 programmatic information and accomplishments relevant to the individual activities within the Office of Technology Development Program for Research, Development, Demonstration, Testing, and Evaluation (RDDT&E). A brief discussion of the mission of the Office of Environmental Restoration and Waste Management (EM) and the Office of Technology Development is presented. An overview is presented of the major problem areas confronting DOE. These problem areas include: groundwater and soils cleanup; waste retrieval and processing; and pollution prevention. The organizational elements within EM are highlighted. An EM-50 Funding Summary for FY92 and FY93 is also provided. RDDT&E programs are discussed and their key problem areas are summarized. Three salient program-formulating concepts are explained. They are: Integrated Demonstrations, Integrated Programs, and the technology window of opportunity. Detailed information for each of the programs within RDDT&E is presented and includes a fact sheet, a list of technical task plans and an accomplishments and objectives section.

  16. Office of Technology Development FY 1993 program summary: Office of Research and Development, Office of Demonstration, Testing and Evaluation. Revision 1

    International Nuclear Information System (INIS)

    This report summarizes significant FY93 programmatic information and accomplishments relevant to the individual activities within the Office of Technology Development Program for Research, Development, Demonstration, Testing, and Evaluation (RDDT ampersand E). A brief discussion of the mission of the Office of Environmental Restoration and Waste Management (EM) and the Office of Technology Development is presented. An overview is presented of the major problem areas confronting DOE. These problem areas include: groundwater and soils cleanup; waste retrieval and processing; and pollution prevention. The organizational elements within EM are highlighted. An EM-50 Funding Summary for FY92 and FY93 is also provided. RDDT ampersand E programs are discussed and their key problem areas are summarized. Three salient program-formulating concepts are explained. They are: Integrated Demonstrations, Integrated Programs, and the technology window of opportunity. Detailed information for each of the programs within RDDT ampersand E is presented and includes a fact sheet, a list of technical task plans and an accomplishments and objectives section

  17. Programmed cell death during development of cowpea (Vigna unguiculata (L.) Walp.) seed coat.

    Science.gov (United States)

    Lima, Nathália Bastos; Trindade, Fernanda Gomes; da Cunha, Maura; Oliveira, Antônia Elenir Amâncio; Topping, Jennifer; Lindsey, Keith; Fernandes, Kátia Valevski Sales

    2015-04-01

    The seed coat develops primarily from maternal tissues and comprises multiple cell layers at maturity, providing a metabolically dynamic interface between the developing embryo and the environment during embryogenesis, dormancy and germination of seeds. Seed coat development involves dramatic cellular changes, and the aim of this research was to investigate the role of programmed cell death (PCD) events during the development of seed coats of cowpea [Vigna unguiculata (L.) Walp.]. We demonstrate that cells of the developing cowpea seed coats undergo a programme of autolytic cell death, detected as cellular morphological changes in nuclei, mitochondria, chloroplasts and vacuoles, DNA fragmentation and oligonucleosome accumulation in the cytoplasm, and loss of membrane viability. We show for the first time that classes 6 and 8 caspase-like enzymes are active during seed coat development, and that these activities may be compartmentalized by translocation between vacuoles and cytoplasm during PCD events. PMID:25142352

  18. New Technology Demonstration Program - Results of an Attempted Field Test of Full-Spectrum Polarized Lighting in a Mail Processing/Office Space

    Energy Technology Data Exchange (ETDEWEB)

    Richman, Eric E.

    2001-06-14

    An assessment of the potential energy savings associated with the use of full-spectrum polarized lighting in a work space was initiated as part of the Department of Energy's (DOE) Federal Energy Management Program (FEMP) New Technology Demonstration Program (NTDP) in 1997. This project was intended to provide information on the effectiveness and application of this technology that could help federal energy managers and other interested individuals determine whether this technology had benefits for their occupied spaces. The use of an actual mail processing/office work area provided the capability of evaluating the technologies effectiveness in the real world.

  19. New Technology Demonstration Program - Results of an Attempted Field Test of Multi-Layer Light Polarizing Panels in an Office Space

    Energy Technology Data Exchange (ETDEWEB)

    Richman, Eric E.

    2001-06-14

    An assessment of the potential energy savings associated with the use of multi-layer light polarizing panels in an office space was initiated as part of the Department of Energy's (DOE) Federal Energy Management Program (FEMP) New Technology Demonstration Program (NTDP) in 1997. This project was intended to provide information on the effectiveness and application of this technology that could help federal energy managers and other interested individuals determine whether this technology had benefits for their occupied spaces. The use of an actual working office area provided the capability of evaluating the technology's effectiveness in the real world.

  20. Industrial Fuel Gas Demonstration-Plant Program. Volume II. The environment (Deliverable No. 27). [Baseline environmental data

    Energy Technology Data Exchange (ETDEWEB)

    1979-08-01

    The proposed site of the Industrial Fuel Gas Demonstration Plant (IFGDP) is located on a small peninsula extending eastward into Lake McKeller from the south shore. The peninsula is located west-southwest of the City of Memphis near the confluence of Lake McKeller and the Mississippi River. The environmental setting of this site and the region around this site is reported in terms of physical, biological, and human descriptions. Within the physical description, this report divides the environmental setting into sections on physiography, geology, hydrology, water quality, climatology, air quality, and ambient noise. The biological description is divided into sections on aquatic and terrestrial ecology. Finally, the human environment description is reported in sections on land use, demography, socioeconomics, culture, and visual features. This section concludes with a discussion of physical environmental constraints.

  1. Using optimization models to demonstrate the need for structural changes in training programs for surgical medical residents.

    Science.gov (United States)

    Turner, Jonathan; Kim, Kibaek; Mehrotra, Sanjay; DaRosa, Debra A; Daskin, Mark S; Rodriguez, Heron E

    2013-09-01

    The primary goal of a residency program is to prepare trainees for unsupervised care. Duty hour restrictions imposed throughout the prior decade require that residents work significantly fewer hours. Moreover, various stakeholders (e.g. the hospital, mentors, other residents, educators, and patients) require them to prioritize very different activities, often conflicting with their learning goals. Surgical residents' learning goals include providing continuity throughout a patient's pre-, peri-, and post-operative care as well as achieving sufficient surgical experience levels in various procedure types and participating in various formal educational activities, among other things. To complicate matters, senior residents often compete with other residents for surgical experience. This paper features experiments using an optimization model and a real dataset. The experiments test the viability of achieving the above goals at a major academic center using existing models of delivering medical education and training to surgical residents. It develops a detailed multi-objective, two-stage stochastic optimization model with anticipatory capabilities solved over a rolling time horizon. A novel feature of the models is the incorporation of learning curve theory in the objection function. Using a deterministic version of the model, we identify bounds on the achievement of learning goals under existing training paradigms. The computational results highlight the structural problems in the current surgical resident educational system. These results further corroborate earlier findings and suggest an educational system redesign is necessary for surgical medical residents.

  2. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1981-01-01

    Provides procedures for demonstrations: (1) the ferrioxalate actinometer, which demonstrates a photochemical reaction; and (2) the silver mirror, which demonstrates the reduction of a metal salt to the metal and/or the reducing power of sugars. (CS)

  3. The immunohistochemical demonstration of parafollicular cells and evaluation of calcium-phosphate balance in patients with thyroid hemiagenesis

    Directory of Open Access Journals (Sweden)

    Jerzy Sowinski

    2011-07-01

    Full Text Available Thyroid hemiagenesis (TH is characterized by the congenital absence of one thyroid lobe. The aim of this study was to evaluate the calcium-phosphate balance in TH. Twenty patients with TH and 20 controls with a bilobed thyroid were studied. Serum concentrations of total calcium, parathormon and calcitonin were measured. Additionally, the immunohistochemical expression of calcitonin, chromogranin A (chA, neuron-specific enolase (NSE and calcitonin gene-related peptide (CGRP was evaluated in surgical specimens from patients with TH and controls. There were no significant differences in biochemical parameters between TH and controls. Positive staining for calcitonin was demonstrated in 3/8 thyroid sections from three patients with TH, but only in 2/33 sections from four controls (p < 0.005. All sections from patients with TH positive for calcitonin also expressed chA, NSE and CGRP. Two sections from controls positive for calcitonin presented an additionally positive reaction for chA, and one of them also for NSE. None presented positive staining for CGRP. Of three TH sections, in one, hyperplasia of C cells of medium grade, and in another hyperplasia of C cells of high grade, could be detected. In the controls, hyperplasia of C cells of low and medium grade was observed. TH was associated with slightly enhanced C cells hyperplasia compared to controls, which might indicate compensatory proliferation. However, the calcium-phosphate balance does not seem to be significantly affected. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 299–305

  4. Ion channels involved in cell volume regulation: effects on migration, proliferation, and programmed cell death in non adherent EAT cells and adherent ELA cells.

    Science.gov (United States)

    Hoffmann, Else Kay

    2011-01-01

    This mini review outlines studies of cell volume regulation in two closely related mammalian cell lines: nonadherent Ehrlich ascites tumour cells (EATC) and adherent Ehrlich Lettre ascites (ELA) cells. Focus is on the regulatory volume decrease (RVD) that occurs after cell swelling, the volume regulatory ion channels involved, and the mechanisms (cellular signalling pathways) that regulate these channels. Finally, I shall also briefly review current investigations in these two cell lines that focuses on how changes in cell volume can regulate cell functions such as cell migration, proliferation, and programmed cell death.

  5. DARPA/USAF/USN J-UCAS X-45A System Demonstration Program: A Review of Flight Test Site Processes and Personnel

    Science.gov (United States)

    Cosentino, Gary B.

    2008-01-01

    The Joint Unmanned Combat Air Systems (J-UCAS) program is a collaborative effort between the Defense Advanced Research Project Agency (DARPA), the US Air Force (USAF) and the US Navy (USN). Together they have reviewed X-45A flight test site processes and personnel as part of a system demonstration program for the UCAV-ATD Flight Test Program. The goal was to provide a disciplined controlled process for system integration and testing and demonstration flight tests. NASA's Dryden Flight Research Center (DFRC) acted as the project manager during this effort and was tasked with the responsibilities of range and ground safety, the provision of flight test support and infrastructure and the monitoring of technical and engineering tasks. DFRC also contributed their engineering knowledge through their contributions in the areas of autonomous ground taxi control development, structural dynamics testing and analysis and the provision of other flight test support including telemetry data, tracking radars, and communications and control support equipment. The Air Force Flight Test Center acted at the Deputy Project Manager in this effort and was responsible for the provision of system safety support and airfield management and air traffic control services, among other supporting roles. The T-33 served as a J-UCAS surrogate aircraft and demonstrated flight characteristics similar to that of the the X-45A. The surrogate served as a significant risk reduction resource providing mission planning verification, range safety mission assessment and team training, among other contributions.

  6. Evaluation of the proposed pilot groundwater pump and treat demonstration for the Paducah Gaseous Diffusion Plant. Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    Bodenstein, G.W.; Bonczek, R.R.; Early, T.O.; Hale, T.B.; Huff, D.D.; Nickelson, M.D.; Rightmire, C.T.

    1992-11-01

    This report contains the evaluation and recommendations of a Groundwater Corrective Actions Review Team. The primary goal is to evaluate the technical merit of and the need to implement a proposed groundwater pump-and-treat demonstration project for the Northwest contaminant plume at Paducah, Kentucky. A key distinction recognized by the review team is that the proposed project is intended to be a full-scale hydraulic containment of contaminants migrating from the sources of the plume, not plume remediation. The key questions incorporated into this plan are whether (1) dense, nonaqueous-phase liquids (DNAPLS) are present in the Regional Gravel Aquifer (RGA) at the source of the plume and (2) {sup 99}Tc removal must be included as part of any groundwater treatment process. The first question cannot be answered until the contaminant sources are better defined; the second question requires further risk assessment and/or a policy decision by DOE. Technical evaluation by the review team suggests that the recommended course of action be to modify the proposed work plan to include accurate identification of the sources of contaminants and vertical distribution of contaminants within the Northwest plume before a decision is made on the preferred source-control option. If DNAPLs are not present in the RGA, removal or containment of the sources is recommended. If DNAPLs are present, then hydraulic containment will be required. Finally, the review team recognizes that it is necessary to initiate a more comprehensive analysis of sitewide remediation needs to create links between action taken for the Northwest plume and action taken for other contamination sites at PGPD.

  7. Cyclosporin A inhibits programmed cell death and cytochrome c release induced by fusicoccin in sycamore cells.

    Science.gov (United States)

    Contran, N; Cerana, R; Crosti, P; Malerba, M

    2007-01-01

    Programmed cell death plays a vital role in normal plant development, response to environmental stresses, and defense against pathogen attack. Different types of programmed cell death occur in plants and the involvement of mitochondria is still under investigation. In sycamore (Acer pseudoplatanus L.) cultured cells, the phytotoxin fusicoccin induces cell death that shows apoptotic features, including chromatin condensation, DNA fragmentation, and release of cytochrome c from mitochondria. In this work, we show that cyclosporin A, an inhibitor of the permeability transition pore of animal mitochondria, inhibits the cell death, DNA fragmentation, and cytochrome c release induced by fusicoccin. In addition, we show that fusicoccin induces a change in the shape of mitochondria which is not prevented by cyclosporin A. These results suggest that the release of cytochrome c induced by fusicoccin occurs through a cyclosporin A-sensitive system that is similar to the permeability transition pore of animal mitochondria and they make it tempting to speculate that this release may be involved in the phytotoxin-induced programmed cell death of sycamore cells.

  8. The NASA Airborne Snow Observatory: Demonstration Mission-3 and the Path Forward to a Broader ASO Program

    Science.gov (United States)

    Painter, T. H.

    2015-12-01

    The NASA Airborne Snow Observatory (ASO), an imaging spectrometer and imaging LiDAR system, to quantify snow water equivalent and snow albedo, provide unprecedented knowledge of snow properties, and provide complete, robust inputs to snowmelt runoff models, water management models, and systems of the future. This talk presents results from the third Demonstration Mission that occurred during the intense California drought of spring 2015, a snow year far worse than the previously worst snow year on record of 2014, and an overview of the various analyses that are finally available due to the uniqueness of the ASO data. In 2015, ASO provided complete basin coverage for the Tuolumne, Merced, Lakes, Rush Creek, and Middle+South Forks of Kings River Basins in the California Sierra Nevada and the Upper Rio Grande, Conejos, and Uncompahgre Basins in the Colorado Rocky Mountains. ASO performed its first wintertime acquisitions in the Tuolumne Basin in response to water managers' needs to quantify SWE volume in what was already realized as dire conditions. Analyses show that with ASO data, river flows and reservoir inflows from the ASO acquisition date to 1 July can be estimated with uncertainties of less than 2%. These results provide enormous value in management operational flexibility for the diversity of needs, and provide strong scientific constraints on the physical processes controlling snowmelt runoff. Snowmelt runoff models are markedly better constrained due to the now accurate knowledge of the distribution of snow water equivalent. With the ASO high-resolution spectrometer and lidar data for a snow-free acquisition, we can determine surface classifications, vegetation heights, and river networks. These data allow runoff models to be accurately and rapidly developed with unprecedented accuracy. These data are now being used to constrain models of varying complexity. Finally, we discuss the path forward on expanding ASO to cover the entire Sierra Nevada and the

  9. Programmed cell death in barley aleurone cells is not directly stimulated by reactive oxygen species produced in response to gibberellin.

    Science.gov (United States)

    Aoki, Nozomi; Ishibashi, Yushi; Kai, Kyohei; Tomokiyo, Reisa; Yuasa, Takashi; Iwaya-Inoue, Mari

    2014-05-01

    The cereal aleurone layer is a secretory tissue that produces enzymes to hydrolyze the starchy endosperm during germination. We recently demonstrated that reactive oxygen species (ROS), produced in response to gibberellins (GA), promoted GAMyb expression, which induces α-amylase expression in barley aleurone cells. On the other hand, ROS levels increase during programmed cell death (PCD) in barley aleurone cells, and GAMyb is involved in PCD of these cells. In this study, we investigated whether the ROS produced in response to GA regulate PCD directly by using mutants of Slender1 (SLN1), a DELLA protein that negatively regulates GA signaling. The wild-type, the sln1c mutant (which exhibits gibberellin-type signaling even in the absence of GA), and the Sln1d mutant (which is gibberellin-insensitive with respect to α-amylase production) all produced ROS in response to GA, suggesting that ROS production in aleurone cells in response to GA is independent of GA signaling through this DELLA protein. Exogenous GA promoted PCD in the wild-type. PCD in sln1c was induced even without exogenous GA (and so without induction of ROS), whereas PCD in Sln1d was not induced in the presence of exogenous GA, even though the ROS content increased significantly in response to GA. These results suggest that PCD in barley aleurone cells is not directly stimulated by ROS produced in response to GA but is regulated by GA signaling through DELLA protein.

  10. DEMONSTRATION OF THE DWPF FLOWSHEET IN THE SRNL SHIELDED CELLS WITH TANK 40 AND H CANYON NEPTUNIUM

    Energy Technology Data Exchange (ETDEWEB)

    Pareizs, J; Bradley Pickenheim, B; Cj Bannochie, C; Michael Stone, M

    2009-04-28

    The Defense Waste Processing Facility (DWPF) is currently processing Sludge Batch 5 (SB5) from Tank 40. SB5 contains the contents of Tank 51 from November 2008, qualified by the Savannah River National Laboratory (SRNL) and the heel in Tank 40 remaining from Sludge Batch 4. Current Liquid Waste Operations (LWO) plans are to (1) decant supernatant from Tank 40 to remove excess liquid caused by a leaking slurry pump and (2) receive a Np stream from H Canyon It should be noted that the Np stream contains significant nitrate requiring addition of nitrite to Tank 40 to maintain a high nitrite to nitrate ratio for corrosion control. SRNL has been requested to qualify the proposed changes; determine the impact on DWPF processability in terms of hydrogen generation, rheology, etc.; evaluate antifoam addition strategy; and evaluate mercury stripping. Therefore, SRNL received a 3 L sample of Tank 40 following the transfer of Tank 51 to Tank 40 (Tank Farm Sample HTF-40-08-157 to be used in testing and to perform the required Waste Acceptance Product Specifications radionuclide analyses). Based on Tank Farm projections, SRNL decanted a portion* of the sample, added sodium nitrite, and added a Np solution from H Canyon representative of the Np to be dispositioned to Tank 40 (neutralized to 0.6 M excess hydroxide). The resulting material was used in a DWPF Chemical Process Cell (CPC) demonstration -- a Sludge Receipt and Adjustment Tank (SRAT) cycle and a Slurry Mix Evaporator (SME) cycle. Preliminary data from the demonstration has been reported previously. This report includes discussion of these results and additional results, including comparisons to Tank Farm projections and the SB5 demonstration.

  11. Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy

    Directory of Open Access Journals (Sweden)

    Julio C. Aguila

    2012-01-01

    success of clinical applications depends on our ability to steer pluripotent stem cells towards the right neuronal identity. In Parkinson disease, the loss of dopamine neurons is more pronounced in the ventrolateral population that projects to the sensorimotor striatum. Because synapses are highly specific, only neurons with this precise identity will contribute, upon transplantation, to the synaptic reconstruction of the dorsal striatum. Thus, understanding the developmental cell program of the mesostriatal dopamine neurons is critical for the identification of the extrinsic signals and cell-intrinsic factors that instruct and, ultimately, determine cell identity. Here, we review how extrinsic signals and transcription factors act together during development to shape midbrain cell fates. Further, we discuss how these same factors can be applied in vitro to induce, select, and reprogram cells to the mesostriatal dopamine fate.

  12. The Australian Hydrogen and Fuel Cells Education Program

    International Nuclear Information System (INIS)

    The next generation of engineers and scientists will face great technical, economic and political challenges to satisfy increasing demands for a secure, reliable and affordable global energy system that maintains and enhances current standards of living. The Australian Hydrogen and Fuel Cells Education Program aims to bolster the quality and relevance of primary and secondary school teaching in emerging areas of science, technology and environmental/sustainability studies using hydrogen, in its capacity as a versatile energy carrier, as the educational basis for teacher and student learning. Critical advances in specific areas of hydrogen production, distribution, storage and end-use technologies arise when students are engaged to develop and apply a broad range of disciplinary and interdisciplinary knowledge and practical skills. A comprehensive hydrogen and fuel cell technology teaching module will be developed to complement existing fuels and energy curricula across Australian schools. The pilot program will be delivered via the collaboration of nine trial schools, a broad range of technical and pedagogy experts and representatives of professional bodies and industry. The program features essential and extensive teacher consultation, a professional learning and development course, industry site visits and a dedicated research and evaluation study. This initiative aims to bolster teacher literacy and student participation in the design, construction and operation of various hydrogen and fuel cell devices and extended activities. Students will reflect on and formally present their learning experiences via several dedicated fora including an awards ceremony where outstanding performance of leading schools, teachers and student groups within the cluster will be acknowledged. (authors)

  13. The Australian Hydrogen and Fuel Cells Education Program

    Energy Technology Data Exchange (ETDEWEB)

    Luigi Bonadio [Senior Consultant Luigi Bonadio and Associates (Australia)

    2006-07-01

    The next generation of engineers and scientists will face great technical, economic and political challenges to satisfy increasing demands for a secure, reliable and affordable global energy system that maintains and enhances current standards of living. The Australian Hydrogen and Fuel Cells Education Program aims to bolster the quality and relevance of primary and secondary school teaching in emerging areas of science, technology and environmental/sustainability studies using hydrogen, in its capacity as a versatile energy carrier, as the educational basis for teacher and student learning. Critical advances in specific areas of hydrogen production, distribution, storage and end-use technologies arise when students are engaged to develop and apply a broad range of disciplinary and interdisciplinary knowledge and practical skills. A comprehensive hydrogen and fuel cell technology teaching module will be developed to complement existing fuels and energy curricula across Australian schools. The pilot program will be delivered via the collaboration of nine trial schools, a broad range of technical and pedagogy experts and representatives of professional bodies and industry. The program features essential and extensive teacher consultation, a professional learning and development course, industry site visits and a dedicated research and evaluation study. This initiative aims to bolster teacher literacy and student participation in the design, construction and operation of various hydrogen and fuel cell devices and extended activities. Students will reflect on and formally present their learning experiences via several dedicated fora including an awards ceremony where outstanding performance of leading schools, teachers and student groups within the cluster will be acknowledged. (authors)

  14. Methicillin resistant Staphylococcus aureus adhesion to human umbilical vein endothelial cells demonstrates wall shear stress dependent behaviour

    Directory of Open Access Journals (Sweden)

    Martinuzzi Robert M

    2011-03-01

    Full Text Available Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA is an increasingly prevalent pathogen capable of causing severe vascular infections. The goal of this work was to investigate the role of shear stress in early adhesion events. Methods Human umbilical vein endothelial cells (HUVEC were exposed to MRSA for 15-60 minutes and shear stresses of 0-1.2 Pa in a parallel plate flow chamber system. Confocal microscopy stacks were captured and analyzed to assess the number of MRSA. Flow chamber parameters were validated using micro-particle image velocimetry (PIV and computational fluid dynamics modelling (CFD. Results Under static conditions, MRSA adhered to, and were internalized by, more than 80% of HUVEC at 15 minutes, and almost 100% of the cells at 1 hour. At 30 minutes, there was no change in the percent HUVEC infected between static and low flow (0.24 Pa, but a 15% decrease was seen at 1.2 Pa. The average number of MRSA per HUVEC decreased 22% between static and 0.24 Pa, and 37% between 0.24 Pa and 1.2 Pa. However, when corrected for changes in bacterial concentration near the surface due to flow, bacteria per area was shown to increase at 0.24 Pa compared to static, with a subsequent decline at 1.2 Pa. Conclusions This study demonstrates that MRSA adhesion to endothelial cells is strongly influenced by flow conditions and time, and that MSRA adhere in greater numbers to regions of low shear stress. These areas are common in arterial bifurcations, locations also susceptible to generation of atherosclerosis.

  15. Immunohistochemical demonstration of EphA2 processing by MT1-MMP in invasive cutaneous squamous cell carcinoma.

    Science.gov (United States)

    Tatsukawa, Ryoko; Koga, Kaori; Aoki, Mikiko; Koshikawa, Naohiko; Imafuku, Shinichi; Nakayama, Juichiro; Nabeshima, Kazuki

    2016-07-01

    Erythropoietin-producing hepatocellular receptor-2 (EphA2) overexpression is prevalent in many types of human cancers, and it has been reported that high EphA2 expression is correlated with malignancy. Recent studies revealed that processing of EphA2 by cleaving off the N-terminal portion by membrane-type 1 matrix metalloproteinase (MT1-MMP) promotes invasion via stimulation of Ras in cancer cells in vitro. The objectives of this study were to investigate the presence and role of EphA2 processing in cutaneous squamous cell carcinoma (SCC) tissues. EphA2 (C-terminal and N-terminal) and MT1-MMP expression patterns and levels were analyzed immunohistochemically in SCC (n = 70) and Bowen disease (BD; n = 20). Levels of MT1-MMP and EphA2 expression were evaluated using digital image analysis. Proximity between MT1-MMP and EphA2 in cancer cells and its effect on EphA2 processing were investigated using a combination of in situ proximity ligation assay (PLA) and Western blotting. Immunohistochemical analyses showed that levels of EphA2 N-terminal expression were significantly lower than those of EphA2 C-terminal expression in SCC, whereas levels of EphA2 C- and N-terminal expression were similar in BD. Western blotting showed processed EphA2 fragments in human SCC tissues. Expression levels of MT1-MMP, EphA2, and processed EphA2 fragments were higher in SCC than BD. Proximity between MT1-MMP and EphA2 in SCC was demonstrated by in situ PLA. Our results suggest possible involvement of MT1-MMP processing of EphA2 in invasiveness of cutaneous SCC. PMID:27056569

  16. Catching up with solid tumor oncology: what is the evidence for a prognostic role of programmed cell death-ligand 1/programmed cell death-1 expression in B-cell lymphomas?

    Science.gov (United States)

    McClanahan, Fabienne; Sharp, Thomas G.; Gribben, John G.

    2016-01-01

    Therapeutic strategies targeting the programmed cell death-ligand 1/programmed cell death-1 pathway have shown significant responses and good tolerability in solid malignancies. Although preclinical studies suggest that inhibiting programmed cell death-ligand 1/programmed cell death-1 interactions might also be highly effective in hematological malignancies, remarkably few clinical trials have been published. Determining patients who will benefit most from programmed cell death-ligand 1/programmed cell death-1-directed immunotherapy and whether programmed cell death-ligand 1/programmed cell death-1 are adequate prognostic markers becomes an increasingly important clinical question, especially as aberrant programmed cell death-ligand 1/programmed cell death-1 expression are key mediators of impaired anti-tumor immune responses in a range of B-cell lymphomas. Herein, we systematically review the published literature on the expression and prognostic value of programmed cell death-ligand 1/programmed cell death-1 in these patients and identify considerable differences in expression patterns, distribution and numbers of programmed cell death-ligand 1+/programmed cell death-1+cells, both between and within lymphoma subtypes, which is reflected in conflicting findings regarding the prognostic value of programmed cell death-ligand 1+/programmed cell death-1+ cells. This can be partly explained by differences in methodologies (techniques, protocols, cutoff values) and definitions of positivity. Moreover, lymphomagenesis, disease progression, and prognosis appear to be determined not only by the presence, numbers and distribution of specific subtypes of T cells, but also by other cells and additional immune checkpoints. Collectively, our findings indicate that programmed cell death-ligand 1/programmed cell death-1 interactions play an essential role in B-cell lymphoma biology and are of clinical importance, but that the overall outcome is determined by additional components

  17. Programmed Cell Death Progresses Differentially in Epidermal and Mesophyll Cells of Lily Petals.

    Directory of Open Access Journals (Sweden)

    Hiroko Mochizuki-Kawai

    Full Text Available In the petals of some species of flowers, programmed cell death (PCD begins earlier in mesophyll cells than in epidermal cells. However, PCD progression in each cell type has not been characterized in detail. We separately constructed a time course of biochemical signs and expression patterns of PCD-associated genes in epidermal and mesophyll cells in Lilium cv. Yelloween petals. Before visible signs of senescence could be observed, we found signs of PCD, including DNA degradation and decreased protein content in mesophyll cells only. In these cells, the total proteinase activity increased on the day after anthesis. Within 3 days after anthesis, the protein content decreased by 61.8%, and 22.8% of mesophyll cells was lost. A second peak of proteinase activity was observed on day 6, and the number of mesophyll cells decreased again from days 4 to 7. These biochemical and morphological results suggest that PCD progressed in steps during flower life in the mesophyll cells. PCD began in epidermal cells on day 5, in temporal synchrony with the time course of visible senescence. In the mesophyll cells, the KDEL-tailed cysteine proteinase (LoCYP and S1/P1 nuclease (LoNUC genes were upregulated before petal wilting, earlier than in epidermal cells. In contrast, relative to that in the mesophyll cells, the expression of the SAG12 cysteine proteinase homolog (LoSAG12 drastically increased in epidermal cells in the final stage of senescence. These results suggest that multiple PCD-associated genes differentially contribute to the time lag of PCD progression between epidermal and mesophyll cells of lily petals.

  18. Programmed cell death of Ulmus pumila L. seeds during aging

    Institute of Scientific and Technical Information of China (English)

    Yulan ZHANG; Ming ZHANG; Fang LI; Xiaofeng WANG

    2008-01-01

    The programmed cell death (PCD) character-istics of Ulmus pumila L. seeds were investigated. The seeds were treated at a high temperature of 37℃ and 100% relative humidity for six days. DAPI (4'6-diami-dino-2-phenylindole) staining revealed that the aging treatment induced condensation and margination of chro-matin, as well as the formation of apoptotic bodies. DNA electrophoresis results of U. pumila seeds on an agarose gel showed a characteristic "ladder" pattern. Levels of electrolyte leakage of seed cells showed that membranes retained their integral form during almost the entire aging time. There was an immediate increase in the production rate of superoxide anion (O2-) and in the amount of hydrogen peroxide (H2O2), which remained at a μmol level. All of these common characteristics indicate that seed aging can be classified as PCD.

  19. Program Area of Interest: Fuel Transformer Solid Oxide Fuel Cell

    Energy Technology Data Exchange (ETDEWEB)

    Norman Bessette; Douglas S. Schmidt; Jolyon Rawson; Rhys Foster; Anthony Litka

    2006-02-01

    The following report documents the technical approach and conclusions made by Acumentrics Corporation during latest budget period toward the development of a low cost 10kW tubular SOFC power system. The present program, guided under direction from the National Energy Technology Laboratory of the US DOE, is a nine-year cost shared Cooperative Agreement totaling close to $74M funded both by the US DOE as well as Acumentrics Corporation and its partners. The latest budget period ran from July of 2005 through December 2005. Work focused on cell technology enhancements as well as BOP and power electronics improvements and overall system design. Significant progress was made in increasing cell power enhancements as well as decreasing material cost in a drive to meet the SECA cost targets. The following report documents these accomplishments in detail as well as the layout plans for further progress in next budget period.

  20. Using microfluidics to study programmed cell death: A new approach

    DEFF Research Database (Denmark)

    Mark, Christina; Zor, Kinga; Heiskanen, Arto;

    This project focuses on applying microfluidic tissue culture for electrochemical or optical measurements during programmed cell death (PCD) in barley aleurone layer to increase understanding of the underlying mechanisms of PCD in plants. Microfluidic tissue culture enables in vitro experiments......, we are optimising an intracellular whole-cell redox activity assay3 that detects changes in redox activity in barley aleurone layer during PCD. The assay uses a double mediator-system to electrochemically measure redox activity via changes in the NADP:NADPH ratio. Initial experiments assay show...... that the redox activity changes depending on phytohormone activation or inactivation of aleurone layer metabolism and subsequent PCD. This is similar to H2O2 concentration changes observed recently by Ishibashi et al4. We have also successfully detected PCD induced by phytohormones in barley aleurone layer using...

  1. Anomalous cell surface structure of sickle cell anemia erythrocytes as demonstrated by cell surface labeling and endo-beta-galactosidase treatment

    Energy Technology Data Exchange (ETDEWEB)

    Fukuda, M.; Fukuda, M.N.; Hakomori, S.; Papayannopoulou, T.

    1981-01-01

    Erythrocyte surface glycoproteins from patients with various types of sickle cell anemia have been analyzed and compared with those from normal individuals. By hemagglutination with various anti-carbohydrate antibodies, sickle cells showed profound increase of i antigens and moderate increase of GlcNAc beta 1 leads to 3Gal beta 1 leads to 3 Glc structure, whereas antigenicity toward globosidic structure was unchanged. In parallel to these findings, erythrocytes of sickle cell patients have additional sialylated lactosaminoglycan in Band 3. Thus, it can be concluded that erythrocytes of sickle cell patients are characterized by an altered cell surface structure which does not appear to be due to topographical changes of cell surface membrane. It is possible that the anemia or the ''stress'' hematopoiesis in these patients is responsible for these changes.

  2. Modulation of Programmed Cell Death in a Model System of Xylogenic Zinnia (Zinnia Elegans) Cell Culture

    NARCIS (Netherlands)

    Yakimova, E.T.; Woltering, E.J.

    2009-01-01

    Programmed cell death is an integral part of the latest stage of differentiation of the tracheary elements of plant xylem vascular system. In this study, by applying a pharmacological approach with specific peptide inhibitors, we have elucidated the involvement of plant caspase-like proteases in cel

  3. Calculus Demonstrations Using MATLAB

    Science.gov (United States)

    Dunn, Peter K.; Harman, Chris

    2002-01-01

    The note discusses ways in which technology can be used in the calculus learning process. In particular, five MATLAB programs are detailed for use by instructors or students that demonstrate important concepts in introductory calculus: Newton's method, differentiation and integration. Two of the programs are animated. The programs and the…

  4. Photodynamic therapy-induced programmed cell death in carcinoma cell lines

    Science.gov (United States)

    He, Xiao-Yan; Sikes, Robert A.; Thomsen, Sharon L.; Chung, L.; Jacques, Steven L.

    1993-06-01

    The mode of cell death following photodynamic therapy (PDT) was investigated from the perspective of programmed cell death (apoptosis). Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a), and rat mammary carcinoma (MTF7) were treated by PDT following sensitization with dihematoporphyrin ether (DHE). The response of these carcinoma cell lines to PDT was variable. An examination of extracted cellular DNA by gel electrophoresis showed the characteristic DNA ladder pattern indicative of internucleosomal cleavage of DNA during apoptosis. MTF7 and PC3 responded to PDT by inducing apoptosis while H322a had no apoptotic response. The magnitude of the response and the PDT dosage required to induce the effect were different in PC3 and MTF7. MTF7 cells responded with rapid apoptosis at the dose of light and drug that yielded 50% cell death (LD50). In contrast, PC3 showed only marginal apoptosis at the LD50 but had a marked response at the LD85. Furthermore, the onset of apoptosis followed slower kinetics in PC3 (2 hr - 4 hr) than in MTF7 (cells were killed by PDT but failed to exhibit any apoptotic response. This study indicates that apoptosis may occur during PDT induced cell death, but this pathway is not universal for all cancer cell lines.

  5. Fuel cell program - Overview reports 2007; Programm Brennstoffzellen inkl. Wasserstoff - Ueberblicksberichte der BFE-Programmleiter 2007

    Energy Technology Data Exchange (ETDEWEB)

    Luzzi, A.; Spirig, M.

    2008-07-01

    This report for the Swiss Federal Office of Energy (SFOE) presents the overview reports made by SFOE Heads of Program on work done in 2007. Projects reported on in the natural gas-fired fuel cell area include the EU-project REAL-SFOC, the long-term testing of anode-supported SOFC stacks, intermediate-temperature fuel cells based on proton conducting electrolytes, the interdisciplinary ONEBAT project and lifetime-enhancement of SOFC stacks for CHP applications. In the polymer-electrolyte fuel cell (PEFC) area, projects concerning proton-conducting polymer membranes, factors limiting the lifetime of fuel cell membranes, a new highly active oxygen reduction electrode for PEM fuel cell and zinc/air battery applications, the enhancement of PEFC durability and reliability, model-based investigation of PEFC performance, and local gas analysis of PE fuel cells are briefly reported on. Long-term research activities in the hydrogen technology area reported on include those concerning the photo-chemical conversion and storage of solar energy and the storage of hydrogen in metallic and complex hydrides. Further projects reported on include those concerning the physical aspects of hydrides for system integration and safety and new, complex metal hydrides. Swiss national and international co-ordination is reviewed in the areas of fuel cell technology and hydrogen technology. Work done in several projects run within the framework of the IEA's Advanced Fuel Cells Program is reviewed. Several pilot and demonstration (P and D) projects are also reported on in the natural-gas SOFC and PEFC areas. Comments on the 2007 results and a review of work to be done in 2008, along with a list of R, D, P and D projects, complete the report.

  6. Apocynin attenuates cholesterol oxidation product-induced programmed cell death by suppressing NF-κB-mediated cell death process in differentiated PC12 cells.

    Science.gov (United States)

    Lee, Da Hee; Nam, Yoon Jeong; Lee, Chung Soo

    2015-10-01

    Cholesterol oxidation products are suggested to be involved in neuronal degeneration. Apocynin has demonstrated to have anti-inflammatory and anti-oxidant effects. We assessed the effect of apocynin on the cholesterol oxidation product-induced programmed cell death in neuronal cells using differentiated PC12 cells in relation to NF-κB-mediated cell death process. 7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3). 7-Ketocholesterol caused an increase in the levels of cytosolic and nuclear NF-κB p65, cytosolic NF-κB p50 and cytosolic phospho-IκB-α, which was inhibited by the addition of 0.5 μM Bay11-7085 (an inhibitor of NF-κB activation). Apocynin attenuated the cholesterol oxidation product-induced changes in the programmed cell death-related protein levels, NF-κB activation, production of reactive oxygen species, and depletion of GSH. The results show that apocynin appears to attenuate the cholesterol oxidation product-induced programmed cell death in PC12 cells by suppressing the activation of the mitochondrial pathway and the caspase-8- and Bid-dependent pathways that are mediated by NF-κB activation. The preventive effect appears to be associated with the inhibitory effect on the production of reactive oxygen species and depletion of GSH.

  7. 324 and 325 Building hot cell cleanout program: Decontamination of C-Cell

    International Nuclear Information System (INIS)

    During FY 1989 the decontamination of C-Cell of Hanford's 324 Building was completed as part of the 324 and 325 Building Hot Cell Cleanout Program sponsored by the DOE Nuclear Energy's Surplus Facilities Management Program. The decontamination effort was completed using a series of remote and contact decontamination techniques. Initial radiation readings in C-Cell averaged 50 rad/hr and were reduced remotely to less than 200 mrad/hr using an alkaline foam cleaner followed by a 5000-psi water flush. Contact decontamination was then permissible using ultra high-pressure water, at 36,000 psi, further reducing the average radiation level in the cell to less than 86 mrem/hr. The approach used in decontaminating C-Cell resulted in a savings in radiation exposure of 87% and a cost savings of 39% compared to a hands-on procedure used in A-Cell, 324 Building in 1987. The radiation dose and the costs to achieve a 244-fold reduction in radiation contamination were 1.65 mrem per ft2 and $96 per ft2 of cell surface area. 14 figs., 4 tabs

  8. 324 and 325 Building hot cell cleanout program: Decontamination of C-Cell

    Energy Technology Data Exchange (ETDEWEB)

    Katayama, Y.B.; Holton, L.K. Jr.

    1989-10-01

    During FY 1989 the decontamination of C-Cell of Hanford's 324 Building was completed as part of the 324 and 325 Building Hot Cell Cleanout Program sponsored by the DOE Nuclear Energy's Surplus Facilities Management Program. The decontamination effort was completed using a series of remote and contact decontamination techniques. Initial radiation readings in C-Cell averaged 50 rad/hr and were reduced remotely to less than 200 mrad/hr using an alkaline foam cleaner followed by a 5000-psi water flush. Contact decontamination was then permissible using ultra high-pressure water, at 36,000 psi, further reducing the average radiation level in the cell to less than 86 mrem/hr. The approach used in decontaminating C-Cell resulted in a savings in radiation exposure of 87% and a cost savings of 39% compared to a hands-on procedure used in A-Cell, 324 Building in 1987. The radiation dose and the costs to achieve a 244-fold reduction in radiation contamination were 1.65 mrem per ft{sup 2} and $96 per ft{sup 2} of cell surface area. 14 figs., 4 tabs.

  9. Solid State Energy Conversion Alliance (SECA) Solid Oxide Fuel Cell Program

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen Minh

    2006-07-31

    This report summarizes the work performed for Phase I (October 2001 - August 2006) under Cooperative Agreement DE-FC26-01NT41245 for the U. S. Department of Energy, National Energy Technology Laboratory (DOE/NETL) entitled 'Solid State Energy Conversion Alliance (SECA) Solid Oxide Fuel Cell Program'. The program focuses on the development of a low-cost, high-performance 3-to-10-kW solid oxide fuel cell (SOFC) system suitable for a broad spectrum of power-generation applications. During Phase I of the program significant progress has been made in the area of SOFC technology. A high-efficiency low-cost system was designed and supporting technology developed such as fuel processing, controls, thermal management, and power electronics. Phase I culminated in the successful demonstration of a prototype system that achieved a peak efficiency of 41%, a high-volume cost of $724/kW, a peak power of 5.4 kW, and a degradation rate of 1.8% per 500 hours. . An improved prototype system was designed, assembled, and delivered to DOE/NETL at the end of the program. This prototype achieved an extraordinary peak efficiency of 49.6%.

  10. The Majorana Demonstrator Radioassay Program

    CERN Document Server

    Abgrall, N; Avignone, F T; Back, H O; Barabash, A S; Bertrand, F E; Boswell, M; Bradley, A W; Brudanin, V; Busch, M; Buuck, M; Byram, D; Caldwell, A S; Chan, Y-D; Christofferson, C D; Chu, P -H; Cuesta, C; Detwiler, J A; Dunmore, J A; Efremenko, Yu; Ejiri, H; Elliott, S R; Finnerty, P; Galindo-Uribarri, A; Gehman, V M; Gilliss, T; Giovanetti, G K; Goett, J; Green, M P; Gruszko, J; Guinn, I S; Guiseppe, V E; Henning, R; Hoppe, E W; Howard, S; Howe, M A; Jasinski, B R; Johnson, R A; Keeter, K J; Kidd, M F; Kochetoi, O; Konovalov, S I; Kouzes, R T; LaFerriere, B D; Leon, J; Loach, J C; MacMullin, J; MacMullin, S; Martin, R D; Massarczyk, R; Meijer, S; Mertens, S; Miller, M L; Orrell, J L; OShaughnessy, C; Overman, N R; Poon, A W P; Pushkin, K; Radford, D C; Rager, J; Rielage, K; Robertson, R G H; Romero-Romero, E; Ronquest, M C; Schubert, A G; Shanks, B; Shirchenko, M; Snavely, K J; Snyder, N; Steele, D; Suriano, A M; Tedeschi, D; Trimble, J E; Varner, R L; Vasilyev, S; Vetter, K; Vorren, K; White, B R; Wilkerson, J F; Wiseman, C; Xu, W; Yakushev, E; Yu, C -H; Yumatov, V; Zhitnikov, I

    2016-01-01

    The MAJORANA collaboration is constructing the MAJORANA DEMONSTATOR at the Sanford Underground Research Facility at the Homestake gold mine, in Lead, SD. The apparatus will use Ge detectors, enriched in isotope \

  11. A role for programmed cell death in the microbial loop.

    Directory of Open Access Journals (Sweden)

    Mónica V Orellana

    Full Text Available The microbial loop is the conventional model by which nutrients and minerals are recycled in aquatic eco-systems. Biochemical pathways in different organisms become metabolically inter-connected such that nutrients are utilized, processed, released and re-utilized by others. The result is that unrelated individuals end up impacting each others' fitness directly through their metabolic activities. This study focused on the impact of programmed cell death (PCD on a population's growth as well as its role in the exchange of carbon between two naturally co-occurring halophilic organisms. Flow cytometric, biochemical, ¹⁴C radioisotope tracing assays, and global transcriptomic analyses show that organic algal photosynthate released by Dunalliela salina cells undergoing PCD complements the nutritional needs of other non-PCD D. salina cells. This occurs in vitro in a carbon limited environment and enhances the growth of the population. In addition, a co-occurring heterotroph Halobacterium salinarum re-mineralizes the carbon providing elemental nutrients for the mixoheterotrophic chlorophyte. The significance of this is uncertain and the archaeon can also subsist entirely on the lysate of apoptotic algae. PCD is now well established in unicellular organisms; however its ecological relevance has been difficult to decipher. In this study we found that PCD in D. salina causes the release of organic nutrients such as glycerol, which can be used by others in the population as well as a co-occurring halophilic archaeon. H. salinarum also re-mineralizes the dissolved material promoting algal growth. PCD in D. salina was the mechanism for the flow of dissolved photosynthate between unrelated organisms. Ironically, programmed death plays a central role in an organism's own population growth and in the exchange of nutrients in the microbial loop.

  12. Targeted Gene Deletion Demonstrates that Cell Adhesion MoleculeICAM-4 is Critical for Erythroblastic Island Formation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Gloria; Lo, Annie; Short, Sarah A.; Mankelow, Tosti J.; Spring, Frances; Parsons, Stephen F.; Mohandas, Narla; Anstee, David J.; Chasis, Joel Anne

    2006-02-15

    Erythroid progenitors differentiate in erythroblastic islands, bone marrow niches composed of erythroblasts surrounding a central macrophage. Evidence suggests that within islands adhesive interactions regulate erythropoiesis and apoptosis. We are exploring whether erythroid intercellular adhesion molecule-4 (ICAM-4), animmunoglobulin superfamily member, participates in island formation. Earlier, we identified alpha V integrins as ICAM-4 counter receptors. Since macrophages express alpha V, ICAM-4 potentially mediates island attachments. To test this, we generated ICAM-4 knockout mice and developed quantitative, live cell techniques for harvesting intact islands and for reforming islands in vitro. We observed a 47 percent decrease in islands reconstituted from ICAM-4 null marrow compared to wild type. We also found a striking decrease in islands formed in vivo in knockout mice. Further, peptides that block ICAM-4 alpha V adhesion produced a 53-57 percent decrease in reconstituted islands, strongly suggesting that ICAM-4 binding to macrophage alpha V functions in island integrity. Importantly, we documented that alpha V integrin is expressed in macrophages isolated from erythro blastic islands. Collectively, these data provide convincing evidence that ICAM-4 is critical in erythroblastic island formation via ICAM-4/alpha V adhesion and also demonstrate that the novel experimental strategies we developed will be valuable in exploring molecular mechanisms of erythroblastic island formation and their functional role in regulating erythropoiesis.

  13. Sludge Washing And Demonstration Of The DWPF Flowsheet In The SRNL Shielded Cells For Sludge Batch 8 Qualification

    Energy Technology Data Exchange (ETDEWEB)

    Pareizs, J. M.; Crawford, C. L.

    2013-04-26

    The current Waste Solidification Engineering (WSE) practice is to prepare sludge batches in Tank 51 by transferring sludge from other tanks to Tank 51. Tank 51 sludge is washed and transferred to Tank 40, the current Defense Waste Processing Facility (DWPF) feed tank. Prior to transfer of Tank 51 to Tank 40, the Savannah River National Laboratory (SRNL) typically simulates the Tank Farm and DWPF processes using a Tank 51 sample (referred to as the qualification sample). WSE requested the SRNL to perform characterization on a Sludge Batch 8 (SB8) sample and demonstrate the DWPF flowsheet in the SRNL shielded cells for SB8 as the final qualification process required prior to SB8 transfer from Tank 51 to Tank 40. A 3-L sample from Tank 51 (the SB8 qualification sample; Tank Farm sample HTF-51-12-80) was received by SRNL on September 20, 2012. The as-received sample was characterized prior to being washed. The washed material was further characterized and used as the material for the DWPF process simulation including a Sludge Receipt and Adjustment Tank (SRAT) cycle, a Slurry Mix Evaporator (SME) cycle, and glass fabrication and chemical durability measurements.

  14. The U.S. Department of Energy, Office of Fossil Energy Stationary Fuel Cell Program

    Science.gov (United States)

    Williams, Mark C.; Strakey, Joseph P.; Surdoval, Wayne A.

    The U.S. Department of Energy (DOE) Office of Fossil Energy's (FE) National Energy Technology Laboratory (NETL), in partnership with private industries, is leading a program for the development and demonstration of high efficiency solid oxide fuel cells (SOFCs) and fuel cell/turbine hybrid power generation systems for near-term distributed generation markets, with emphasis on premium power and high reliability. NETL is partnering with Pacific Northwest National Laboratory (PNNL) in developing new directions for research under the Solid State Energy Conversion Alliance (SECA) initiative to develop and commercialize modular, low cost, and fuel flexible SOFC systems. Through advanced materials, processing and system integration research and development (R&D), the SECA initiative will reduce the fuel cell cost to $400 kW -1 for stationary and auxiliary power unit markets. The SECA industry teams and core program have made significant progress in scale-up and performance. Presidential initiatives are focusing research toward a new hydrogen economy. The movement to a hydrogen economy would accomplish several strategic goals, namely that SOFCs have no emissions, and hence figure significantly in DOE strategies. The SOFC hybrid is a key part of the FutureGen plant, a major new DOE FE initiative to produce hydrogen from coal. The highly efficient SOFC hybrid plant will produce electric power while other parts of the plant could produce hydrogen and sequester CO 2. The produced hydrogen can be used in fuel cell cars and for SOFC distributed generation applications.

  15. Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells.

    Science.gov (United States)

    Monteiro, Rui; Pinheiro, Philip; Joseph, Nicola; Peterkin, Tessa; Koth, Jana; Repapi, Emmanouela; Bonkhofer, Florian; Kirmizitas, Arif; Patient, Roger

    2016-08-22

    Hematopoietic stem cells (HSCs) are self-renewing multipotent stem cells that generate mature blood lineages throughout life. They, together with hematopoietic progenitor cells (collectively known as HSPCs), emerge from hemogenic endothelium in the floor of the embryonic dorsal aorta by an endothelial-to-hematopoietic transition (EHT). Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). The requirement for TGFβ is two fold and sequential: autocrine via Tgfβ1a and Tgfβ1b produced in the endothelial cells themselves, followed by a paracrine input of Tgfβ3 from the notochord, suggesting that the former programs the hemogenic endothelium and the latter drives EHT. Our findings have important implications for the generation of HSPCs from pluripotent cells in vitro. PMID:27499523

  16. SLUDGE WASHING AND DEMONSTRATION OF THE DWPF FLOWSHEET IN THE SRNL SHIELDED CELLS FOR SLUDGE BATCH 6 QUALIFICATION

    Energy Technology Data Exchange (ETDEWEB)

    Pareizs, J.; Pickenheim, B.; Bannochie, C.; Billings, A.; Bibler, N.; Click, D.

    2010-10-01

    Prior to initiating a new sludge batch in the Defense Waste Processing Facility (DWPF), Savannah River National Laboratory (SRNL) is required to simulate this processing, including Chemical Process Cell (CPC) simulation, waste glass fabrication, and chemical durability testing. This report documents this simulation for the next sludge batch, Sludge Batch 6 (SB6). SB6 consists of Tank 12 material that has been transferred to Tank 51 and subjected to Low Temperature Aluminum Dissolution (LTAD), Tank 4 sludge, and H-Canyon Pu solutions. Following LTAD and the Tank 4 addition, Liquid Waste Operations (LWO) provided SRNL a 3 L sample of Tank 51 sludge for SB6 qualification. Pu solution from H Canyon was also received. SB6 qualification included washing the sample per LWO plans/projections (including the addition of Pu from H Canyon), DWPF CPC simulations, waste glass fabrication (vitrification), and waste glass characterization and chemical durability evaluation. The following are significant observations from this demonstration. Sludge settling improved slightly as the sludge was washed. SRNL recommended (and the Tank Farm implemented) one less wash based on evaluations of Tank 40 heel projections and projections of the glass composition following transfer of Tank 51 to Tank 40. Thorium was detected in significant quantities (>0.1 wt % of total solids) in the sludge. In past sludge batches, thorium has been determined by Inductively Coupled Plasma-Mass Spectroscopy (ICP-MS), seen in small quantities, and reported with the radionuclides. As a result of the high thorium, SRNL-AD has added thorium to their suite of Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES) elements. The acid stoichiometry for the DWPF Sludge Receipt and Adjustment Tank (SRAT) processing of 115%, or 1.3 mol acid per liter of SRAT receipt slurry, was adequate to accomplish some of the goals of SRAT processing: nitrite was destroyed to below 1,000 mg/kg and mercury was removed to

  17. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1986-01-01

    Provides three descriptions of demonstrations used in various chemistry courses. Includes the use of a simple demonstration model to illustrate principles of chromatography, techniques for using balloons to teach about the behavior of gases, and the use of small concentrations of synthetic polyelectrolytes to induce the flocculation hydrophobic…

  18. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1987-01-01

    Describes two laboratory demonstrations in chemistry. One uses dry ice, freon, and freezer bags to demonstrate volume changes, vapor-liquid equilibrium, a simulation of a rain forest, and vaporization. The other uses the clock reaction technique to illustrate fast reactions and kinetic problems in releasing carbon dioxide during respiration. (TW)

  19. Complete Demonstration.

    Science.gov (United States)

    Yelon, Stephen; Maddocks, Peg

    1986-01-01

    Describes four-step approach to educational demonstration: tell learners they will have to perform; what they should notice; describe each step before doing it; and require memorization of steps. Examples illustrate use of this process to demonstrate a general mental strategy, and industrial design, supervisory, fine motor, and specific…

  20. Electrodril system field test program. Phase II: Task C-1-deep drilling system demonstration. Final report for Phase II: Task C-1

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, P D

    1981-04-01

    The Electrodril Deep Drilling System field test demonstrations were aborted in July 1979, due to connector problems. Subsequent post test analyses concluded that the field replacable connectors were the probable cause of the problems encountered. The designs for both the male and female connectors, together with their manufacturing processes, were subsequently modified, as was the acceptance test procedures. A total of nine male and nine female connectors were manufactured and delivered during the 2nd Quarter 1980. Exhaustive testing was then conducted on each connector as a precursor to formal qualification testing conducted during the month of October 1980, at the Brown Oil Tool test facility located in Houston, Texas. With this report, requirements under Phase II, Task C-1 are satisfied. The report documents the results of the connector qualification test program which was successfully completed October 28, 1980. In general, it was concluded that connector qualification had been achieved and plans are now in progress to resume the field test demonstration program so that Electrodril System performance predictions and economic viability can be evaluated.

  1. Cystoisospora canis (Apicomplexa: Sarcocystidae): development of monozoic tissue cysts in human cells, demonstration of egress of zoites from tissue cysts, and demonstration of repeat monozoic tissue cyst formation by zoites.

    Science.gov (United States)

    Houk, Alice E; Lindsay, David S

    2013-11-01

    Sporozoites of Cystoisospora canis penetrated and developed to monozoic tissue cysts in 4 human, 1 monkey, 1 bovine and 2 canine cell lines. No asexual division was documented although multiple infection of a single cell was observed. Examination of cultures using transmission electron microscopy demonstrated that they were monozoic tissue cysts and contained a single sporozoite. The appearance of monozoic tissue cysts in all cell lines was similar but the parasitophorous vacuole surrounding some sporozoites in DH82 dog macrophages was swollen. Monozoic tissue cysts were observed for up to 127 days in human pigmented retinal epithelial cells. Treatment of cell cultures containing monozoic tissue cysts with 0.75 sodium taurocholic acid and 0.25% trypsin stimulated egress of zoites (former sporozoites) from tissue cysts. Zoites collected from monozoic tissue cysts were able to penetrate and develop to monozoic tissue cysts in new host cells. Monozoic tissue cysts survived exposure to acid pepsin solution indicating that they would be orally infectious. The tissue cyst wall surrounding zoites did not autofluoresce as did oocyst and sporocyst walls exposed to UV light. We believe that C. canis can be used as a model system to study extra-intestinal monozoic tissue cysts stages of Cystoisospora belli of humans.

  2. The Bacillus cereus spoIIS programmed cell death system

    Directory of Open Access Journals (Sweden)

    Jana eMelnicakova

    2015-08-01

    Full Text Available Programmed cell death in bacteria is generally associated with two¬ component toxin antitoxin systems. The SpoIIS toxin-antitoxin system, consisting of a membrane bound SpoIISA toxin and a small, cytosolic antitoxin SpoIISB, was originally identified in Bacillus subtilis. In this work we describe the Bacillus cereus SpoIIS system which is a three-component system, harbouring an additional gene spoIISC. Its protein product serves as an antitoxin, and similarly as SpoIISB, is able to bind SpoIISA and abolish its toxic effect. Our results indicate that SpoIISC seems to be present not only in B. cereus but also in other Bacilli containing a SpoIIS toxin antitoxin system. In addition, we show that B. cereus SpoIISA can form higher oligomers and we discuss the possible role of this multimerization for the protein’s toxic function.

  3. Programmed cell death in developing human fetal CNS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The spatial and temporal distributions of programmed cell death (PCD) in developing central nervous system (CNS) of human fetuses ranging from 12 to 39 weeks of gestation were investigated using techniques of flow cytometry and terminal transferase-mediated nick end labeling (TUNEL). The results showed that PCD did occur in every representative brain region of all fetuses examined in different stages. It was found that there were two peaks of PCD appearing at the 12th and 39th weeks respectively, which suggested that the first peak of apoptosis may be involved in the selective elimination of neurons overproduced during the early development and the second may play an important role in establishing the correct neuronal circuitry.

  4. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L.

    1990-01-01

    Included are three demonstrations that include the phase change of ice when under pressure, viscoelasticity and colloid systems, and flame tests for metal ions. The materials, procedures, probable results, and applications to real life situations are included. (KR)

  5. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1987-01-01

    Presents three demonstrations suitable for undergraduate chemistry classes. Focuses on experiments with calcium carbide, the induction by iron of the oxidation of iodide by dichromate, and the classical iodine clock reaction. (ML)

  6. Irreversible dual inhibitory mode: the novel Btk inhibitor PLS-123 demonstrates promising anti-tumor activity in human B-cell lymphoma

    OpenAIRE

    Ding, Ning; Li, Xitao; Shi, Yunfei; Ping, Lingyan; Wu, Lina; Fu, Kai; Feng, Lixia; Zheng, Xiaohui; Song, Yuqin; Pan, Zhengying; Zhu, Jun

    2015-01-01

    The B-cell receptor (BCR) signaling pathway has gained significant attention as a therapeutic target in B-cell malignancies. Recently, several drugs that target the BCR signaling pathway, especially the Btk inhibitor ibrutinib, have demonstrated notable therapeutic effects in relapsed/refractory patients, which indicates that pharmacological inhibition of BCR pathway holds promise in B-cell lymphoma treatment. Here we present a novel covalent irreversible Btk inhibitor PLS-123 with more poten...

  7. The Molecular Ecophysiology of Programmed Cell Death in Marine Phytoplankton

    Science.gov (United States)

    Bidle, Kay D.

    2015-01-01

    Planktonic, prokaryotic, and eukaryotic photoautotrophs (phytoplankton) share a diverse and ancient evolutionary history, during which time they have played key roles in regulating marine food webs, biogeochemical cycles, and Earth's climate. Because phytoplankton represent the basis of marine ecosystems, the manner in which they die critically determines the flow and fate of photosynthetically fixed organic matter (and associated elements), ultimately constraining upper-ocean biogeochemistry. Programmed cell death (PCD) and associated pathway genes, which are triggered by a variety of nutrient stressors and are employed by parasitic viruses, play an integral role in determining the cell fate of diverse photoautotrophs in the modern ocean. Indeed, these multifaceted death pathways continue to shape the success and evolutionary trajectory of diverse phytoplankton lineages at sea. Research over the past two decades has employed physiological, biochemical, and genetic techniques to provide a novel, comprehensive, mechanistic understanding of the factors controlling this key process. Here, I discuss the current understanding of the genetics, activation, and regulation of PCD pathways in marine model systems; how PCD evolved in unicellular photoautotrophs; how it mechanistically interfaces with viral infection pathways; how stress signals are sensed and transduced into cellular responses; and how novel molecular and biochemical tools are revealing the impact of PCD genes on the fate of natural phytoplankton assemblages.

  8. Anhydrobiosis and programmed cell death in plants: Commonalities and Differences

    Directory of Open Access Journals (Sweden)

    Samer Singh

    2015-05-01

    Full Text Available Anhydrobiosis is an adaptive strategy of certain organisms or specialised propagules to survive in the absence of water while programmed cell death (PCD is a finely tuned cellular process of the selective elimination of targeted cell during developmental programme and perturbed biotic and abiotic conditions. Particularly during water stress both the strategies serve single purpose i.e., survival indicating PCD may also function as an adaptive process under certain conditions. During stress conditions PCD cause targeted cells death in order to keep the homeostatic balance required for the organism survival, whereas anhydrobiosis suspends cellular metabolic functions mimicking a state similar to death until reestablishment of the favourable conditions. Anhydrobiosis is commonly observed among organisms that have ability to revive their metabolism on rehydration after removal of all or almost all cellular water without damage. This feature is widely represented in terrestrial cyanobacteria and bryophytes where it is very common in both vegetative and reproductive stages of life-cycle. In the course of evolution, with the development of advanced vascular system in higher plants, anhydrobiosis was gradually lost from the vegetative phase of life-cycle. Though it is retained in resurrection plants that primarily belong to thallophytes and a small group of vascular angiosperm, it can be mostly found restricted in orthodox seeds of higher plants. On the contrary, PCD is a common process in all eukaryotes from unicellular to multicellular organisms including higher plants and mammals. In this review we discuss physiological and biochemical commonalities and differences between anhydrobiosis and PCD.

  9. Hepatoma cell line HepG2.2.15 demonstrates distinct biological features compared with parental HepG2

    Institute of Scientific and Technical Information of China (English)

    Ran Zhao; Tian-Zhen Wang; Dan Kong; Lei Zhang; Hong-Xue Meng; Yang Jiang; Yi-Qi Wu; Zu-Xi Yu; Xiao-Ming Jin

    2011-01-01

    AIM: To investigate the biological features of hepatitis B virus (HBV)-transfected HepG2.2.15 cells. METHODS: The cell ultrastructure, cell cycle and apop-tosis, and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy, flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay. Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice, and the pathological analysis of tumor formation was performed. Two cytoskeletal proteins were detected by Western blotting.RESULTS: Compared with HepG2 cells, HepG2.2.15 cells showed organelle degeneration and filopodia disappear-ance under electron microscope. HepG2.2.15 cells pro-liferated and migrated slowly in vitro, and hardly formed tumor and lung metastasis in nude mice. Flow cytom-etry showed that the majority of HepG2.2.15 cells were arrested in G1 phase, and apoptosis was minor in both cell lines. Furthermore, the levels of cytoskeletal pro-teins F-actin and Ezrin were decreased in HepG2.2.15 cells.CONCLUSION: HepG2.2.15 cells demonstrated a low-er proliferation and invasion ability than the HepG2 cells due to HBV transfection.

  10. Programmed cell death features in apple suspension cells under low oxygen culture

    Institute of Scientific and Technical Information of China (English)

    XU Chang-jie(徐昌杰); CHEN Kun-song(陈昆松); FERGUSON Ian B.

    2004-01-01

    Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Protoplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.

  11. Tested Demonstrations.

    Science.gov (United States)

    Gilbert, George L., Ed.

    1985-01-01

    Background information, procedures, and typical results obtained are provided for two demonstrations. The first involves the colorful complexes of copper(II). The second involves reverse-phase separation of Food, Drug, and Cosmetic (FD & C) dyes using a solvent gradient. (JN)

  12. Pathways to Commercial Success. Technologies and Products Supported by the Fuel Cell Technologies Program

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2010-08-01

    This report identifies the commercial and near-commercial (emerging) hydrogen and fuel cell technologies and products that resulted from Department of Energy support through the Fuel Cell Technologies Program in the Office of Energy Efficiency and Renewable Energy.

  13. High consistency forming process for paper making. Part 1. A research, development, and demonstration program plan for the US Papermaking Industry. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1980-04-01

    The subject of research, development, and demonstration (RD and D) of energy conserving technologies applicable to papermaking operations downstream of the pulping process is addressed. An RD and D Program Plan is presented based on a survey of leading representatives of the papermaking industry, equipment manufacturers, consulting engineering firms, the American Paper Institute, and the Technical Association of the Pulp and Paper Industry. For perspective, the program plan is presented against the general background of the industry's current issues and concerns. The second part of the paper, Phase I, final report, deals with papermaking test facilities. The case for a centralized test facility is discussed. The results of a survey of existing pilot paper machines are presented. The energy saving potential of high consistency forming is considered and related to existing evidence. Simple theoretical models for the press nip action and the drying process are developed to predict where high consistency forming will reduce energy consumption. A special dynamic former has been designed, fabricated, and commissioned to allow development of a laboratory high consistency headbox. The design and construction of a low speed headbox has been completed and the complete system operated. Special equipment and techniques for the measurement of the water and air permeability of sheet samples have been developed and are described.

  14. SF Treg cells transcribing high levels of Bcl-2 and microRNA-21 demonstrate limited apoptosis in RA

    NARCIS (Netherlands)

    van der Geest, Kornelis S. M.; Smigielska, Katarzyna; Park, Ji-Ah; Abdulahad, Wayel H.; Kim, Hye-Won; Kroesen, Bart-Jan; van den Berg, Anke; Boots, Annemieke M. H.; Lee, Eun-Bong; Brouwer, Elisabeth

    2015-01-01

    Objective. The aim of this study was to investigate the turnover of Treg cells in the SF of RA patients. Methods. Treg cells were enumerated in peripheral blood and SF of RA patients and analysed by flow cytometry for expression of the proliferation marker Ki-67 and binding of the apoptosis marker a

  15. Demonstration of a visual cell-based assay for screening glucose transporter 4 translocation modulators in real time

    Indian Academy of Sciences (India)

    Maleppillil Vavachan Vijayakumar; Amrendra Kumar Ajay; Manoj Kumar Bhat

    2010-12-01

    Insulin-stimulated translocation of glucose transporter 4 (GLUT4) to cell membrane leading to glucose uptake is the rate-limiting step in diabetes. It is also a defined target of antidiabetic drug research. Existing GLUT4 translocation assays are based on time-consuming immunoassays and are hampered by assay variability and low sensitivity. We describe a real-time, visual, cell-based qualitative GLUT4 translocation assay using CHO-HIRc-myc-GLUT4eGFP cells that stably express myc- and eGFP-tagged GLUT4 in addition to human insulin receptor (HIRc). GLUT4 translocation is visualized by live cell imaging based on GFP fluorescence by employing a cooled charge-coupled device camera attached to a fluorescent microscope. This video imaging method and further quantitative analysis of GLUT4 on the cell membrane provide rapid and foolproof visual evidence that this method is suitable for screening GLUT4 translocation modulators.

  16. Clonal heterogeneity of small-cell anaplastic carcinoma of the lung demonstrated by flow-cytometric DNA analysis

    DEFF Research Database (Denmark)

    Vindeløv, L L; Hansen, H H; Christensen, I J;

    1980-01-01

    Flow-cytometric DNA analysis yields information on ploidy and proliferative characteristics of a cell population. The analysis was implemented on small-cell anaplastic carcinoma of the lung using a rapid detergent technique for the preparation of fine-needle aspirates for DNA determination...... of contamination of the aspirates with normal cells was determined by differential counts. The ratio of the peak channel numbers for the G1 phase of the tumor cells to that of the diploid standard (DNA index) was calculated and used for ploidy identification. Twenty-nine patients were evaluable with respect to DNA...... of the detection limit set by the methodology used and the restricted number of samples studied in each patient indicate that the true occurrence of clonal heterogeneity in small-cell carcinoma of the lung may be much higher....

  17. Energy audit of three energy-conserving devices in a steel-industry demonstration program. Task I. Hague forge furnaces. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Lownie, H.W.; Holden, F.C.

    1982-06-01

    A program to demonstrate to industry the benefits of installing particular types of energy-conserving devices and equipment was carried out. One of these types of equipment and the results obtained under production conditions in commercial plants are described. The equipment under consideration includes improved forge furnaces and associated heat-recovery components. They are used to heat steel to about 2300 F prior to hot forging. The energy-conserving devices include improved insulation, automatic air-fuel ratio control, and a ceramic recuperator that recovers heat from hot combustion gases and delivers preheated air to high-temperature recirculating burners. Twelve Hague furnaces and retrofit packages were purchased and installed by eleven host forge shops that agree to furnish performance data for the purpose of demonstrating the energy and economic savings that can be achieved in comparison with existing equipment. Fuel savings were reported by comparing the specific energy consumption (Btu's per pound of steel heated) for each Hague furnace with that of a comparison furnace. Economic comparisons were made using payback period based on annual after-tax cash flow. Payback periods for the Hague equipment varied from less than two years to five years or more. In several cases, payback times were high only because the units were operated at a small fraction of their available capacity.

  18. Potential for cell therapy in Parkinson's disease using genetically programmed human embryonic stem cell-derived neural progenitor cells.

    Science.gov (United States)

    Ambasudhan, Rajesh; Dolatabadi, Nima; Nutter, Anthony; Masliah, Eliezer; Mckercher, Scott R; Lipton, Stuart A

    2014-08-15

    Neural transplantation is a promising strategy for restoring dopaminergic dysfunction and modifying disease progression in Parkinson's disease (PD). Human embryonic stem cells (hESCs) are a potential resource in this regard because of their ability to provide a virtually limitless supply of homogenous dopaminergic progenitors and neurons of appropriate lineage. The recent advances in developing robust cell culture protocols for directed differentiation of hESCs to near pure populations of ventral mesencephalic (A9-type) dopaminergic neurons has heightened the prospects for PD cell therapy. Here, we focus our review on current state-of-the-art techniques for harnessing hESC-based strategies toward development of a stem cell therapeutic for PD. Importantly, we also briefly describe a novel genetic-programming approach that may address many of the key challenges that remain in the field and that may hasten clinical translation.

  19. Mesenchymal stem cells from cortical bone demonstrate increased clonal incidence, potency, and developmental capacity compared to their bone marrow–derived counterparts

    Directory of Open Access Journals (Sweden)

    Daniel Blashki

    2016-08-01

    Full Text Available In this study, we show that matrix dense cortical bone is the more potent compartment of bone than bone marrow as a stromal source for mesenchymal stem cells as isolated from adult rats. Lineage-depleted cortical bone-mesenchymal stem cells demonstrated >150-fold enrichment of colony forming unit–fibroblasts per cell incidence. compared to lineage-depleted bone marrow-mesenchymal stem cells, corresponding to a 70-fold increase in absolute recovered colony forming unit–fibroblasts. The composite phenotype Lin−/CD45−/CD31−/VLA-1+/Thy-1+ enriched for clonogenic mesenchymal stem cells solely from cortical bone–derived cells from which 70% of clones spontaneously differentiated into all lineages of bone, cartilage, and adipose. Both populations generated vascularized bone tissue within subcutaneous implanted collagen scaffolds; however, cortical bone–derived cells formed significantly more osteoid than bone marrow counterparts, quantified by histology. The data demonstrate that our isolation protocol identifies and validates mesenchymal stem cells with superior clonal, proliferative, and developmental potential from cortical bone compared to the bone marrow niche although marrow persists as the typical source for mesenchymal stem cells both in the literature and current pre-clinical therapies.

  20. Mesenchymal stem cells from cortical bone demonstrate increased clonal incidence, potency, and developmental capacity compared to their bone marrow–derived counterparts

    Science.gov (United States)

    Blashki, Daniel; Murphy, Matthew B; Ferrari, Mauro; Simmons, Paul J; Tasciotti, Ennio

    2016-01-01

    In this study, we show that matrix dense cortical bone is the more potent compartment of bone than bone marrow as a stromal source for mesenchymal stem cells as isolated from adult rats. Lineage-depleted cortical bone-mesenchymal stem cells demonstrated >150-fold enrichment of colony forming unit–fibroblasts per cell incidence. compared to lineage-depleted bone marrow-mesenchymal stem cells, corresponding to a 70-fold increase in absolute recovered colony forming unit–fibroblasts. The composite phenotype Lin−/CD45−/CD31−/VLA-1+/Thy-1+ enriched for clonogenic mesenchymal stem cells solely from cortical bone–derived cells from which 70% of clones spontaneously differentiated into all lineages of bone, cartilage, and adipose. Both populations generated vascularized bone tissue within subcutaneous implanted collagen scaffolds; however, cortical bone–derived cells formed significantly more osteoid than bone marrow counterparts, quantified by histology. The data demonstrate that our isolation protocol identifies and validates mesenchymal stem cells with superior clonal, proliferative, and developmental potential from cortical bone compared to the bone marrow niche although marrow persists as the typical source for mesenchymal stem cells both in the literature and current pre-clinical therapies. PMID:27579159

  1. Antibody-Dependent Cell-Mediated Cytotoxicity Effector-Enhanced EphA2 Agonist Monoclonal Antibody Demonstrates Potent Activity against Human Tumors

    Directory of Open Access Journals (Sweden)

    Elizabeth M. Bruckheimer

    2009-06-01

    Full Text Available EphA2 is a receptor tyrosine kinase that has been shown to be overexpressed in a variety of human tumor types. Previous studies demonstrated that agonist monoclonal antibodies targeting EphA2 induced the internalization and degradation of the receptor, thereby abolishing its oncogenic effects. In this study, the in vitro and in vivo antibody-dependent cell-mediated cytotoxicity (ADCC activity of EphA2 effector-enhanced agonist monoclonal antibodies was evaluated. With tumor cell lines and healthy human peripheral blood monocytes, the EphA2 antibodies demonstrated ∼80% tumor cell killing. In a dose-dependent manner, natural killer (NK cells were required for the in vitro ADCC activity and became activated as demonstrated by the induction of cell surface expression of CD107a. To assess the role of NK cells on antitumor efficacy in vivo, the EphA2 antibodies were evaluated in xenograft models in severe compromised immunodeficient (SCID mice (which have functional NK cells and monocytes and SCID nonobese diabetic (NOD mice (which largely lack functional NK cells and monocytes. Dosing of EphA2 antibody in the SCID murine tumor model resulted in a 6.2-fold reduction in tumor volume, whereas the SCID/nonobese diabetic model showed a 1.6-fold reduction over the isotype controls. Together, these results demonstrate that the anti-EphA2 monoclonal antibodies may function through at least two mechanisms of action: EphA2 receptor activation and ADCC-mediated activity. These novel EphA2 monoclonal antibodies provide additional means by which host effector mechanisms can be activated for selective destruction of EphA2-expressing tumor cells.

  2. Programmed cell death features in apple suspension cells under low oxygen culture

    Institute of Scientific and Technical Information of China (English)

    徐昌杰; 陈昆松; FERGUSONIanB

    2004-01-01

    Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Protoplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple celi death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.

  3. The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity.

    Science.gov (United States)

    Hanschen, Erik R; Marriage, Tara N; Ferris, Patrick J; Hamaji, Takashi; Toyoda, Atsushi; Fujiyama, Asao; Neme, Rafik; Noguchi, Hideki; Minakuchi, Yohei; Suzuki, Masahiro; Kawai-Toyooka, Hiroko; Smith, David R; Sparks, Halle; Anderson, Jaden; Bakarić, Robert; Luria, Victor; Karger, Amir; Kirschner, Marc W; Durand, Pierre M; Michod, Richard E; Nozaki, Hisayoshi; Olson, Bradley J S C

    2016-01-01

    The transition to multicellularity has occurred numerous times in all domains of life, yet its initial steps are poorly understood. The volvocine green algae are a tractable system for understanding the genetic basis of multicellularity including the initial formation of cooperative cell groups. Here we report the genome sequence of the undifferentiated colonial alga, Gonium pectorale, where group formation evolved by co-option of the retinoblastoma cell cycle regulatory pathway. Significantly, expression of the Gonium retinoblastoma cell cycle regulator in unicellular Chlamydomonas causes it to become colonial. The presence of these changes in undifferentiated Gonium indicates extensive group-level adaptation during the initial step in the evolution of multicellularity. These results emphasize an early and formative step in the evolution of multicellularity, the evolution of cell cycle regulation, one that may shed light on the evolutionary history of other multicellular innovations and evolutionary transitions. PMID:27102219

  4. Cardiac Sarcoidosis or Giant Cell Myocarditis? On Treatment Improvement of Fulminant Myocarditis as Demonstrated by Cardiovascular Magnetic Resonance Imaging

    Science.gov (United States)

    Bogabathina, Hari; Olson, Peter; Rathi, Vikas K.; Biederman, Robert W. W.

    2012-01-01

    Giant cell myocarditis, but not cardiac sarcoidosis, is known to cause fulminant myocarditis resulting in severe heart failure. However, giant cell myocarditis and cardiac sarcoidosis are pathologically similar, and attempts at pathological differentiation between the two remain difficult. We are presenting a case of fulminant myocarditis that has pathological features suggestive of cardiac sarcoidosis, but clinically mimicking giant cell myocarditis. This patient was treated with cyclosporine and prednisone and recovered well. This case we believe challenges our current understanding of these intertwined conditions. By obtaining a sense of severity of cardiac involvement via delayed hyperenhancement of cardiac magnetic resonance imaging, we were more inclined to treat this patient as giant cell myocarditis with cyclosporine. This resulted in excellent improvement of patient's cardiac function as shown by delayed hyperenhancement images, early perfusion images, and SSFP videos. PMID:24826266

  5. Cardiac Sarcoidosis or Giant Cell Myocarditis? On Treatment Improvement of Fulminant Myocarditis as Demonstrated by Cardiovascular Magnetic Resonance Imaging

    Directory of Open Access Journals (Sweden)

    Hari Bogabathina

    2012-01-01

    Full Text Available Giant cell myocarditis, but not cardiac sarcoidosis, is known to cause fulminant myocarditis resulting in severe heart failure. However, giant cell myocarditis and cardiac sarcoidosis are pathologically similar, and attempts at pathological differentiation between the two remain difficult. We are presenting a case of fulminant myocarditis that has pathological features suggestive of cardiac sarcoidosis, but clinically mimicking giant cell myocarditis. This patient was treated with cyclosporine and prednisone and recovered well. This case we believe challenges our current understanding of these intertwined conditions. By obtaining a sense of severity of cardiac involvement via delayed hyperenhancement of cardiac magnetic resonance imaging, we were more inclined to treat this patient as giant cell myocarditis with cyclosporine. This resulted in excellent improvement of patient’s cardiac function as shown by delayed hyperenhancement images, early perfusion images, and SSFP videos.

  6. Eosinophilic granuloma of bone and biochemical demonstration of 49-kDa CD1a molecule expression by Langerhans-cell histiocytosis.

    Science.gov (United States)

    Cambazard, F; Dezutter-Dambuyant, C; Staquet, M J; Schmitt, D; Thivolet, J

    1991-09-01

    Histiocytic cells infiltrating the lesions in eosinophilic granuloma of bone as well as in cutaneous histiocytosis X were studied using a murine monoclonal antibody (MA) produced with proliferating cells from an eosinophilic granuloma of bone. This MA reacts with Langerhans cells (LC) of normal human skin or mucous membranes and with proliferating cells of eosinophilic granuloma of bone and skin lesions of Letter-Siwe disease, as shown by immunohistochemistry and immunogold labelling. As other murine MA's obtained after immunization with human cortical thymocytes, this MA immunoprecipitates the 49-kDa CD1a antigen found on human LC and thymic-cell surfaces but not its breakdown product after treatment with trypsin, as demonstrated by analysis of immunoelectron labelling, cytofluorometry and gel electrophoresis. This first production of a CD1a MA from an eosinophilic granuloma supports the concept of Langerhans-cell histiocytosis.

  7. Regulation of programmed cell death by plasminogen activator inhibitor type 1 (PAI-1)

    DEFF Research Database (Denmark)

    Lademann, Ulrik Axel; Rømer, Maria Unni Koefoed

    2008-01-01

    PA) observed in tumours; however, several lines of evidence suggest that PAI-1 may contribute directly to the pathology of the disease. PAI-1 has been reported to have an effect on most of the basic cellular processes including cell adhesion, cell migration, cell invasion, and cell proliferation and increasing...... numbers of reports suggest that PAI-1 also can regulate programmed cell death (PCD) in cancer cells and normal cells. A number of reports suggest that PAI-1 can inhibit PCD through its pro-adhesive/anti-proteolytic property whereas other reports suggest that PAI-1 induces PCD through its anti......-adhesive property.Furthermore,it has been suggested that PAI-1 can either induce or inhibit PCD though activation of cell signalling pathways.This review will focus on the regulation of programmed cell death by PAI-1 in both normal cells and cancer cells....

  8. Commissioning the Majorana Demonstrator

    Science.gov (United States)

    Xu, Wenqin; Majorana Collaboration

    2016-03-01

    The Majorana Demonstrator deploys high purity germanium (HPGe) detector modules to search for neutrinoless double beta (0 νββ) decay in 76Ge. The experiment is aimed at demonstrating the technical feasibility and low backgrounds for a next generation Ge-based BBz experiment. The program of testing and commissioning the Demonstrator modules is a critical step to debug and improve the experimental apparatus, to establish and refine operational procedures, and to develop data analysis tools. In this talk, we will discuss our experience commissioning the Demonstrator modules and show how this program leads to successful data-taking. This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Nuclear Physics, the Particle Astrophysics and Nuclear Physics Programs of the National Science Foundation, and the Sanford Underground Research Facility.

  9. Early interferon-γ production in human lymphocyte subsets in response to nontyphoidal Salmonella demonstrates inherent capacity in innate cells.

    Directory of Open Access Journals (Sweden)

    Tonney S Nyirenda

    Full Text Available BACKGROUND: Nontyphoidal Salmonellae frequently cause life-threatening bacteremia in sub-Saharan Africa. Young children and HIV-infected adults are particularly susceptible. High case-fatality rates and increasing antibiotic resistance require new approaches to the management of this disease. Impaired cellular immunity caused by defects in the T helper 1 pathway lead to intracellular disease with Salmonella that can be countered by IFNγ administration. This report identifies the lymphocyte subsets that produce IFNγ early in Salmonella infection. METHODOLOGY: Intracellular cytokine staining was used to identify IFNγ production in blood lymphocyte subsets of ten healthy adults with antibodies to Salmonella (as evidence of immunity to Salmonella, in response to stimulation with live and heat-killed preparations of the D23580 invasive African isolate of Salmonella Typhimurium. The absolute number of IFNγ-producing cells in innate, innate-like and adaptive lymphocyte subpopulations was determined. PRINCIPAL FINDINGS: Early IFNγ production was found in the innate/innate-like lymphocyte subsets: γδ-T cells, NK cells and NK-like T cells. Significantly higher percentages of such cells produced IFNγ compared to adaptive αβ-T cells (Student's t test, P<0.001 and ≤0.02 for each innate subset compared, respectively, with CD4(+- and CD8(+-T cells. The absolute numbers of IFNγ-producing cells showed similar differences. The proportion of IFNγ-producing γδ-T cells, but not other lymphocytes, was significantly higher when stimulated with live compared with heat-killed bacteria (P<0.0001. CONCLUSION/SIGNIFICANCE: Our findings indicate an inherent capacity of innate/innate-like lymphocyte subsets to produce IFNγ early in the response to Salmonella infection. This may serve to control intracellular infection and reduce the threat of extracellular spread of disease with bacteremia which becomes life-threatening in the absence of protective antibody

  10. In vivo demonstration of red cell-endothelial interaction, sickling and altered microvascular response to oxygen in the sickle transgenic mouse.

    OpenAIRE

    Kaul, D K; Fabry, M E; Costantini, F; E. M. Rubin; Nagel, R L

    1995-01-01

    Intravascular sickling, red cell-endothelium interaction, and altered microvascular responses have been suggested to contribute to the pathophysiology of human sickle cell disease, but have never been demonstrated under in vivo flow. To address this issue, we have examined a transgenic mouse line, alphaHbetaSbetaS-Antilles [betaMDD] which has a combined high (78%) expression of beta S and beta S-Antilles globins. In vivo microcirculatory studies using the cremaster muscle preparation showed a...

  11. 2015 DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Report

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2015-10-01

    This report summarizes comments from the Peer Review Panel at the 2015 DOE Hydrogen and Fuel Cells Program Annual Merit Review, held on June 8-12, 2015, in Arlington, Virginia. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes, and standards; market transformation; and systems analysis.

  12. 2014 DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Report

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2014-10-01

    This report summarizes comments from the Peer Review Panel at the 2014 DOE Hydrogen and Fuel Cells Program Annual Merit Review, held on June 16-20, 2014, in Washington, DC. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes, and standards; market transformation; and systems analysis.

  13. 2011 DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Report

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2011-09-01

    This report summarizes comments from the Peer Review Panel at the 2011 DOE Hydrogen and Fuel Cells Program Annual Merit Review, held on May 9-13, 2011, in Arlington, Virginia. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes, and standards; education; market transformation; and systems analysis.

  14. 2013 DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Report

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2013-10-01

    This report summarizes comments from the Peer Review Panel at the 2013 DOE Hydrogen and Fuel Cells Program Annual Merit Review, held on May 13-17, 2013, in Arlington, Virginia. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes, and standards; market transformation; and systems analysis.

  15. 2012 DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Report

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2012-09-01

    This report summarizes comments from the Peer Review Panel at the 2012 DOE Hydrogen and Fuel Cells Program Annual Merit Review, held on May 14-18, 2012, in Arlington, Virginia. It covers the program areas of hydrogen production and delivery; hydrogen storage; fuel cells; manufacturing R&D; technology validation; safety, codes, and standards; education; market transformation; and systems analysis.

  16. Demonstration of the Presence of the “Deleted” MIR122 Gene in HepG2 Cells

    Science.gov (United States)

    Hamad, Ibrahim A. Y.; Fei, Yue; Kalea, Anastasia Z.; Yin, Dan; Smith, Andrew J. P.; Palmen, Jutta; Humphries, Steve E.; Talmud, Philippa J.; Walker, Ann P.

    2015-01-01

    MicroRNA 122 (miR-122) is highly expressed in the liver where it influences diverse biological processes and pathways, including hepatitis C virus replication and metabolism of iron and cholesterol. It is processed from a long non-coding primary transcript (~7.5 kb) and the gene has two evolutionarily-conserved regions containing the pri-mir-122 promoter and pre-mir-122 hairpin region. Several groups reported that the widely-used hepatocytic cell line HepG2 had deficient expression of miR-122, previously ascribed to deletion of the pre-mir-122 stem-loop region. We aimed to characterise this deletion by direct sequencing of 6078 bp containing the pri-mir-122 promoter and pre-mir-122 stem-loop region in HepG2 and Huh-7, a control hepatocytic cell line reported to express miR-122, supported by sequence analysis of cloned genomic DNA. In contrast to previous findings, the entire sequence was present in both cell lines. Ten SNPs were heterozygous in HepG2 indicating that DNA was present in two copies. Three validation isolates of HepG2 were sequenced, showing identical genotype to the original in two, whereas the third was different. Investigation of promoter chromatin status by FAIRE showed that Huh-7 cells had 6.2 ± 0.19- and 2.7 ± 0.01- fold more accessible chromatin at the proximal (HNF4α-binding) and distal DR1 transcription factor sites, compared to HepG2 cells (p=0.03 and 0.001, respectively). This was substantiated by ENCODE genome annotations, which showed a DNAse I hypersensitive site in the pri-mir-122 promoter in Huh-7 that was absent in HepG2 cells. While the origin of the reported deletion is unclear, cell lines should be obtained from a reputable source and used at low passage number to avoid discrepant results. Deficiency of miR-122 expression in HepG2 cells may be related to a relative deficiency of accessible promoter chromatin in HepG2 versus Huh-7 cells. PMID:25811611

  17. Death of mitochondria during programmed cell death of leaf mesophyll cells.

    Science.gov (United States)

    Selga, Tūrs; Selga, Maija; Pāvila, Vineta

    2005-12-01

    The role of plant mitochondria in the programmed cell death (PCD) is widely discussed. However, spectrum and sequence of mitochondrial structural changes during different types of PCD in leaves are poorly described. Pea, cucumber and rye plants were grown under controlled growing conditions. A part of them were sprinkled with ethylene releaser to accelerate cell death. During yellowing the palisade parenchyma mitochondria were attracted to nuclear envelope. Mitochondrial matrix became electron translucent. Mitochondria entered vacuole by invagination of tonoplast and formed multivesicular bodies. Ethephon treatment increased the frequency of sticking of mitochondria to the nuclear envelope or chloroplasts and peroxisomes. Mitochondria divided by different mechanisms and became enclosed in Golgi and ER derived authopagic vacuoles or in the central vacuole. Several fold increase of the diameter of cristae became typical. In all cases mitochondria were attached to nuclear envelope. It can be considered as structural mechanism of promoting of PCD.

  18. Phosphoproteome analysis demonstrates the potential role of THRAP3 phosphorylation in androgen-independent prostate cancer cell growth.

    Science.gov (United States)

    Ino, Yoko; Arakawa, Noriaki; Ishiguro, Hitoshi; Uemura, Hiroji; Kubota, Yoshinobu; Hirano, Hisashi; Toda, Tosifusa

    2016-04-01

    Elucidating the androgen-independent growth mechanism is critical for developing effective treatment strategies to combat androgen-independent prostate cancer. We performed a comparative phosphoproteome analysis using a prostate cancer cell line, LNCaP, and an LNCaP-derived androgen-independent cell line, LNCaP-AI, to identify phosphoproteins involved in this mechanism. We performed quantitative comparisons of the phosphopeptide levels in tryptic digests of protein extracts from these cell lines using MS. We found that the levels of 69 phosphopeptides in 66 proteins significantly differed between LNCaP and LNCaP-AI. In particular, we focused on thyroid hormone receptor associated protein 3 (THRAP3), which is a known transcriptional coactivator of the androgen receptor. The phosphorylation level of THRAP3 was significantly lower at S248 and S253 in LNCaP-AI cells. Furthermore, pull-down assays showed that 32 proteins uniquely bound to the nonphosphorylatable mutant form of THRAP3, whereas 31 other proteins uniquely bound to the phosphorylation-mimic form. Many of the differentially interacting proteins were identified as being involved with RNA splicing and processing. These results suggest that the phosphorylation state of THRAP3 at S248 and S253 might be involved in the mechanism of androgen-independent prostate cancer cell growth by changing the interaction partners.

  19. Design and experimental demonstration of low-power CMOS magnetic cell manipulation platform using charge recycling technique

    Science.gov (United States)

    Niitsu, Kiichi; Yoshida, Kohei; Nakazato, Kazuo

    2016-03-01

    We present the world’s first charge-recycling-based low-power technique of complementary metal-oxide-semiconductor (CMOS) magnetic cell manipulation. CMOS magnetic cell manipulation associated with magnetic beads is a promissing tool for on-chip biomedical-analysis applications such as drug screening because CMOS can integrate control electronics and electro-chemical sensors. However, the conventional CMOS cell manipulation requires considerable power consumption. In this work, by concatenating multiple unit circuits and recycling electric charge among them, power consumption is reduced by a factor of the number of the concatenated unit circuits (1/N). For verifying the effectiveness, test chip was fabricated in a 0.6-µm CMOS. The chip successfully manipulates magnetic microbeads with achieving 49% power reduction (from 51 to 26.2 mW). Even considering the additional serial resistance of the concatenated inductors, nearly theoretical power reduction effect can be confirmed.

  20. Lessons learned from implementation of a demonstration program to reduce the burden of anemia and hookworm in women in Yen Bai Province, Viet Nam

    Directory of Open Access Journals (Sweden)

    Thach Tran D

    2009-07-01

    Full Text Available Abstract Background Iron deficiency, anemia and hookworm disease are important public health problems for women of reproductive age living in developing countries and affect the health of newborns and infants. Iron supplementation and deworming treatment are effective in addressing these problems in both pregnant and non-pregnant women. Daily iron supplementation and deworming after the first trimester is recommended for pregnant women although these programs usually do not operate efficiently or effectively. Weekly iron-folic acid supplementation and regular deworming for non-pregnant women may be a viable approach for improving iron status and preventing anemia during the reproductive years. Addressing these diseases at a population level before women become pregnant could significantly improve women's health before and during pregnancy, as well as their infants' growth and development. Methods and Results This paper describes the major processes undertaken in a demonstration intervention of preventive weekly iron-folic acid supplementation with regular deworming for all 52,000 women aged 15–45 years in two districts of Yen Bai province, in northern Viet Nam. The intervention strategy included extensive consultation with community leaders and village, commune, district and provincial health staff, and training for village health workers. Distribution of the drugs was integrated with the existing health service infrastructure and the village health workers were the direct point of contact with women. Iron-folic acid tablets and deworming treatment were provided free of charge from May 2006. An independent Vietnamese NGO was commissioned to evaluate compliance and identify potential problems. The program resulted in effective distribution of iron-folic acid tablets and deworming treatment to all villages in the target districts, with full or partial compliance of 85%. Conclusion Training for health staff, the strong commitment of all partners

  1. Within a smoking-cessation program, what impact does genetic information on lung cancer need to have to demonstrate cost-effectiveness?

    Directory of Open Access Journals (Sweden)

    Gordon Louisa G

    2010-09-01

    Full Text Available Abstract Background Many smoking-cessation programs and pharmaceutical aids demonstrate substantial health gains for a relatively low allocation of resources. Genetic information represents a type of individualized or personal feedback regarding the risk of developing lung cancer, and hence the potential benefits from stopping smoking, may motivate the person to remain smoke-free. The purpose of this study was to explore what the impact of a genetic test needs to have within a typical smoking-cessation program aimed at heavy smokers in order to be cost-effective. Methods Two strategies were modelled for a hypothetical cohort of heavy smokers aged 50 years; individuals either received or did not receive a genetic test within the course of a usual smoking-cessation intervention comprising nicotine replacement therapy (NRT and counselling. A Markov model was constructed using evidence from published randomized controlled trials and meta-analyses for estimates on 12-month quit rates and long-term relapse rates. Epidemiological data were used for estimates on lung cancer risk stratified by time since quitting and smoking patterns. Extensive sensitivity analyses were used to explore parameter uncertainty. Results The discounted incremental cost per QALY was AU$34,687 (95% CI $12,483, $87,734 over 35 years. At a willingness-to-pay of AU$20,000 per QALY gained, the genetic testing strategy needs to produce a 12-month quit rate of at least 12.4% or a relapse rate 12% lower than NRT and counselling alone for it to be equally cost-effective. The likelihood that adding a genetic test to the usual smoking-cessation intervention is cost-effective was 20.6% however cost-effectiveness ratios were favourable in certain situations (e.g., applied to men only, a 60 year old cohort. Conclusions The findings were sensitive to small changes in critical variables such as the 12-month quit rates and relapse rates. As such, the cost-effectiveness of the genetic testing

  2. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation

    Institute of Scientific and Technical Information of China (English)

    Jing Jiang; Wenjian Lv; Xiaoying Ye; Lingbo Wang; Man Zhang; Hui Yang; Maja Okuka

    2013-01-01

    Induced pluripotent stem (iPS) cells generated using Yamanaka factors have great potential for use in autologous cell therapy.However,genomic abnormalities exist in human iPS cells,and most mouse iPS cells are not fully pluripotent,as evaluated by the tetraploid complementation assay (TCA); this is most likely associated with the DNA damage response (DDR) occurred in early reprogramming induced by Yamanaka factors.In contrast,nuclear transfer can faithfully reprogram somatic cells into embryonic stem (ES) cells that satisfy the TCA.We thus hypothesized that factors involved in oocyte-induced reprogramming may stabilize the somatic genome during reprogramming,and improve the quality of the resultant iPS cells.To test this hypothesis,we screened for factors that could decrease DDR signals during iPS cell induction.We determined that Zscan4,in combination with the Yamanaka factors,not only remarkably reduced the DDR but also markedly promoted the efficiency of iPS cell generation.The inclusion of Zscan4 stabilized the genomic DNA,resulting in p53 downregulation.Furthermore,Zscan4 also enhanced telomere lengthening as early as 3 days post-infection through a telomere recombination-based mechanism.As a result,iPS cells generated with addition of Zscan4 exhibited longer telomeres than classical iPS cells.Strikingly,more than 50%of iPS cell lines (11/19) produced via this "Zscan4 protocol" gave rise to live-borne all-iPS cell mice as determined by TCA,compared to 1/12 for lines produced using the classical Yamanaka factors.Our findings provide the first demonstration that maintaining genomic stability during reprogramming promotes the generation of high quality iPS cells.

  3. Conditionally immortalized human pancreatic stellate cell lines demonstrate enhanced proliferation and migration in response to IGF-I

    Energy Technology Data Exchange (ETDEWEB)

    Rosendahl, Ann H., E-mail: ann.rosendahl@med.lu.se [Lund University, Department of Clinical Sciences Lund, Division of Surgery, Lund (Sweden); Lund University and Skåne University Hospital, Department of Clinical Sciences Lund, Division of Oncology and Pathology, Lund (Sweden); Gundewar, Chinmay; Said Hilmersson, Katarzyna [Lund University, Department of Clinical Sciences Lund, Division of Surgery, Lund (Sweden); Ni, Lan; Saleem, Moin A. [University of Bristol, School of Clinical Sciences, Children' s Renal Unit and Academic Renal Unit, Bristol (United Kingdom); Andersson, Roland [Lund University, Department of Clinical Sciences Lund, Division of Surgery, Lund (Sweden)

    2015-01-15

    Pancreatic stellate cells (PSCs) play a key role in the dense desmoplastic stroma associated with pancreatic ductal adenocarcinoma. Studies on human PSCs have been minimal due to difficulty in maintaining primary PSC in culture. We have generated the first conditionally immortalized human non-tumor (NPSC) and tumor-derived (TPSC) pancreatic stellate cells via transformation with the temperature-sensitive SV40 large T antigen and human telomerase (hTERT). These cells proliferate at 33°C. After transfer to 37°C, the SV40LT is switched off and the cells regain their primary PSC phenotype and growth characteristics. NPSC contained cytoplasmic vitamin A-storing lipid droplets, while both NPSC and TPSC expressed the characteristic markers αSMA, vimentin, desmin and GFAP. Proteome array analysis revealed that of the 55 evaluated proteins, 27 (49%) were upregulated ≥3-fold in TPSC compared to NPSC, including uPA, pentraxin-3, endoglin and endothelin-1. Two insulin-like growth factor binding proteins (IGFBPs) were inversely expressed. Although discordant IGFBP-2 and IGFBP-3 levels, IGF-I was found to stimulate proliferation of both NPSC and TPSC. Both basal and IGF-I stimulated motility was significantly enhanced in TPSC compared to NPSC. In conclusion, these cells provide a unique resource that will facilitate further study of the active stroma compartment associated with pancreatic cancer. - Highlights: • Generation of human conditionally immortalized human pancreatic stellate cell lines. • Temperature-sensitive SV40LT allows switch to primary PSC phenotype characteristics. • Proteome profiling revealed distinct expression patterns between TPSC and NPSC. • Enhanced IGF-I-stimulated proliferation and motility by TPSC compared to NPSC.

  4. R and D, demonstration and market introduction of SOFC technology at Topsoe Fuel Cell A/S

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, J.B.; Christiansen, N. [Haldor Topsoe A/S and Topsoe Fuel Cell A/S, Lyngby (Denmark)

    2008-07-01

    Solid Oxide Fuel Cell Technology is very promising because it is fuel flexible and can use CH{sub 4} as well as CO directly as fuel in addition to hydrogen. The SOFC technology at Topsoe Fuel Cell A/S and Risoe/ National Laboratory is based on an integrated approach ranging from manufacturing of planar anode-supported cells and compact stacks, development of tailor made fuel processing catalysts to analysis of total systems. The direct use of CH{sub 4} is possible because the anode material is active for steam reforming. The endothermic reforming reaction aids in removing the irreversibly generated heat from the stack and in effect upgrades waste heat to chemical energy in the form of hydrogen. The standard SOFC cells are thin and robust with dimensions of 12 x 12 cm{sup 2} or 18 x 18 cm{sup 2} foot prints. The stack design has been tested for more than 13000 hours with a degradation rate of less than 1% per 1000 hours. Several 50 or 75 cell stacks in the 1+kW power range have been tested successfully at a fuel utilization of up to 92%. Multi stack modules consisting of four 75 cell stacks have been tested for more than 4000 hours with pre-reformed natural gas. The degradation rate has been reduced to below 0.5% per 1000 hours by improvement of metal alloy interconnects and coatings. In collaboration with Waertsilae, a 24-stack prototype based on natural gas is being tested. For methanol based systems the methanol is methanated upstream the anode using a newly developed catalyst. Additional fuels studied include LPG, methanol, DME, diesel and ammonia. The studies predict system electrical efficiencies from 40-56% (AC out/LHV fuel in), depending on the fuel used and the size of the system. The range of fuels has now been extended to include ethanol and synthesis gas from biomass or coal. (orig.)

  5. Cyclophilin A regulates HIV-1 infectivity, as demonstrated by gene targeting in human T cells

    OpenAIRE

    Braaten, Douglas; Luban, Jeremy

    2001-01-01

    The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein binds most members of the cyclophilin family of peptidyl-prolyl isomerases. Of 15 known human cyclophilins, cyclophilin A (CypA) has been the focus of investigation because it was detected in HIV-1 virions. To determine whether CypA promotes HIV-1 replication, we deleted the gene encoding CypA (PPIA) in human CD4+ T cells by homologous recombination. HIV-1 replication in PPIA–/– cells was decreased and not inhibited further by cy...

  6. Drug-induced trafficking of p-glycoprotein in human brain capillary endothelial cells as demonstrated by exposure to mitomycin C.

    OpenAIRE

    Noack, Andreas; Noack, Sandra; Hoffmann, Andrea; Maalouf, Katia; Buettner, Manuela; Couraud, Pierre-Olivier; Romero, Ignacio A.; Weksler, Babette; Alms, Dana; Römermann, Kerstin; Naim, Hassan Y; Löscher, Wolfgang

    2014-01-01

    P-glycoprotein (Pgp; ABCB1/MDR1) is a major efflux transporter at the blood-brain barrier (BBB), restricting the penetration of various compounds. In other tissues, trafficking of Pgp from subcellular stores to the cell surface has been demonstrated and may constitute a rapid way of the cell to respond to toxic compounds by functional membrane insertion of the transporter. It is not known whether drug-induced Pgp trafficking also occurs in brain capillary endothelial cells that form the BBB. ...

  7. Medicare, Medicaid, and Children's Health Insurance Programs: Announcement of the Provider Enrollment Moratoria Access Waiver Demonstration of Part B Non-Emergency Ground Ambulance Suppliers and Home Health Agencies in Moratoria-Designated Geographic Locations. Implementation of the waiver demonstration.

    Science.gov (United States)

    2016-08-01

    This notice announces the Provider Enrollment Moratoria Access Waiver Demonstration of Part B Non-Emergency Ground Ambulance Suppliers and Home Health Agencies in 6 states. The demonstration is being implemented in accordance with section 402 of the Social Security Amendments of 1967 and gives CMS the authority to grant waivers to the statewide enrollment moratoria on a case-by-case basis in response to access to care issues, and to subject providers and suppliers enrolling via such waivers to heightened screening, oversight, and investigations. PMID:27487580

  8. Proteome analysis demonstrates profound alterations in human dendritic cell nature by TX527, an analogue of vitamin D

    DEFF Research Database (Denmark)

    Ferreira, G. B.; van Etten, E.; Lage, K.;

    2009-01-01

    of a tolerogenic cell. In the present study, we aimed to explore the global protein changes induced by the analogue in immature DC (iDC) and mature human DC and to correlate them with alterations in DC morphology and function. Human CD14(+) monocytes were differentiated toward iDC or mature DCs, in the presence...

  9. Cardenolide-Induced Lysosomal Membrane Permeabilization Demonstrates Therapeutic Benefits in Experimental Human Non-Small Cell Lung Cancers

    Directory of Open Access Journals (Sweden)

    Tatjana Mijatovic

    2006-05-01

    Full Text Available Non-small cell lung cancers (NSCLCs are the leading cause of cancer deaths in most developed countries. Targeting heat shock protein 70 (Hsp70 expression and function, together with the induction of lysosomal membrane permeabilization (LMP, could overcome the multiple anti-cell death mechanisms evidenced in NSCLCs that are responsible for the failure of currently used chemotherapeutic drugs. Because cardenolides bind to the sodium pump, they affect multiple signaling pathways and thus have a number of marked effects on tumor cell behavior. The aim of the present study was to characterize in vitro and in vivo the antitumor effects of a new cardenolide (UNBS1450 on experimental human NSCLCs. UNBS1450 is a potent source of in vivo antitumor activity in the case of paclitaxeland oxaliplatin-resistant subcutaneous human NCIH727 and orthotopic A549 xenografts in nude mice. In vitro UNBS1450-mediated antitumor activity results from the induction of nonapoptotic cell death. UNBS1450 mediates the decrease of Hsp70 at both mRNA and protein levels, and this is at least partly due to UNBS1450-induced downregulation of NFAT5/ TonEBP (a factor responsible for the transcriptional control of Hsp70. These effects were paralleled by the induction of LMP, as evidenced by acridine orange staining and immunofluorescence analysis for cathepsin B accumulation.

  10. Grouping annotations on the subcellular layered interactome demonstrates enhanced autophagy activity in a recurrent experimental autoimmune uveitis T cell line.

    Directory of Open Access Journals (Sweden)

    Xiuzhi Jia

    Full Text Available Human uveitis is a type of T cell-mediated autoimmune disease that often shows relapse-remitting courses affecting multiple biological processes. As a cytoplasmic process, autophagy has been seen as an adaptive response to cell death and survival, yet the link between autophagy and T cell-mediated autoimmunity is not certain. In this study, based on the differentially expressed genes (GSE19652 between the recurrent versus monophasic T cell lines, whose adoptive transfer to susceptible animals may result in respective recurrent or monophasic uveitis, we proposed grouping annotations on a subcellular layered interactome framework to analyze the specific bioprocesses that are linked to the recurrence of T cell autoimmunity. That is, the subcellular layered interactome was established by the Cytoscape and Cerebral plugin based on differential expression, global interactome, and subcellular localization information. Then, the layered interactomes were grouping annotated by the ClueGO plugin based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. The analysis showed that significant bioprocesses with autophagy were orchestrated in the cytoplasmic layered interactome and that mTOR may have a regulatory role in it. Furthermore, by setting up recurrent and monophasic uveitis in Lewis rats, we confirmed by transmission electron microscopy that, in comparison to the monophasic disease, recurrent uveitis in vivo showed significantly increased autophagy activity and extended lymphocyte infiltration to the affected retina. In summary, our framework methodology is a useful tool to disclose specific bioprocesses and molecular targets that can be attributed to a certain disease. Our results indicated that targeted inhibition of autophagy pathways may perturb the recurrence of uveitis.

  11. SPACE-R Thermionic Space Nuclear Power System: Design and Technology Demonstration Program. Semiannual technical progress report for period ending March 1993

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    This Semiannual Technical Progress Report summarizes the technical progress and accomplishments for the Thermionic Space Nuclear Power System (TI-SNPS) Design and Technology Demonstration Program of the Prime Contractor, Space Power Incorporated (SPI), its subcontractors and supporting National Laboratories during the first half of the Government Fiscal Year (GFY) 1993. SPI`s subcontractors and supporting National Laboratories include: Babcock & Wilcox for the reactor core and externals; Space Systems/Loral for the spacecraft integration; Thermocore for the radiator heat pipes and the heat exchanger; INERTEK of CIS for the TFE, core elements and nuclear tests; Argonne National Laboratories for nuclear safety, physics and control verification; and Oak Ridge National laboratories for materials testing. Parametric trade studies are near completion. However, technical input from INERTEK has yet to be provided to determine some of the baseline design configurations. The INERTEK subcontract is expected to be initiated soon. The Point Design task has been initiated. The thermionic fuel element (TFE) is undergoing several design iterations. The reactor core vessel analysis and design has also been started.

  12. The role of reservoir characterization in the reservoir management process (as reflected in the Department of Energy`s reservoir management demonstration program)

    Energy Technology Data Exchange (ETDEWEB)

    Fowler, M.L. [BDM-Petroleum Technologies, Bartlesville, OK (United States); Young, M.A.; Madden, M.P. [BDM-Oklahoma, Bartlesville, OK (United States)] [and others

    1997-08-01

    Optimum reservoir recovery and profitability result from guidance of reservoir practices provided by an effective reservoir management plan. Success in developing the best, most appropriate reservoir management plan requires knowledge and consideration of (1) the reservoir system including rocks, and rock-fluid interactions (i.e., a characterization of the reservoir) as well as wellbores and associated equipment and surface facilities; (2) the technologies available to describe, analyze, and exploit the reservoir; and (3) the business environment under which the plan will be developed and implemented. Reservoir characterization is the essential to gain needed knowledge of the reservoir for reservoir management plan building. Reservoir characterization efforts can be appropriately scaled by considering the reservoir management context under which the plan is being built. Reservoir management plans de-optimize with time as technology and the business environment change or as new reservoir information indicates the reservoir characterization models on which the current plan is based are inadequate. BDM-Oklahoma and the Department of Energy have implemented a program of reservoir management demonstrations to encourage operators with limited resources and experience to learn, implement, and disperse sound reservoir management techniques through cooperative research and development projects whose objectives are to develop reservoir management plans. In each of the three projects currently underway, careful attention to reservoir management context assures a reservoir characterization approach that is sufficient, but not in excess of what is necessary, to devise and implement an effective reservoir management plan.

  13. GASIS demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Vidas, E.H. [Energy and Environmental Analysis, Inc., Arlington, VA (United States)

    1995-04-01

    A prototype of the GASIS database and retrieval software has been developed and is the subject of this poster session and computer demonstration. The prototype consists of test or preliminary versions of the GASIS Reservoir Data System and Source Directory datasets and the software for query and retrieval. The prototype reservoir database covers the Rocky Mountain region and contains the full GASIS data matrix (all GASIS data elements) that will eventually be included on the CD-ROM. It is populated for development purposes primarily by the information included in the Rocky Mountain Gas Atlas. The software has been developed specifically for GASIS using Foxpro for Windows. The application is an executable file that does not require Foxpro to run. The reservoir database software includes query and retrieval, screen display, report generation, and data export functions. Basic queries by state, basin, or field name will be assisted by scrolling selection lists. A detailed query screen will allow record selection on the basis of any data field, such as depth, cumulative production, or geological age. Logical operators can be applied to any-numeric data element or combination of elements. Screen display includes a {open_quotes}browse{close_quotes} display with one record per row and a detailed single record display. Datasets can be exported in standard formats for manipulation with other software packages. The Source Directory software will allow record retrieval by database type or subject area.

  14. Establishment and characterization of pleomorphic adenoma cell systems: an in-vitro demonstration of carcinomas arising secondarily from adenomas in the salivary gland

    International Nuclear Information System (INIS)

    Among the salivary gland carcinomas, carcinoma in pleomorphic adenoma has been regarded as a representative carcinoma type which arises secondarily in the background of a pre-existent benign pleomorphic adenoma. It is still poorly understood how and which benign pleomorphic adenoma cells transform into its malignant form, carcinoma ex pleomorphic adenoma. We have established five cell systems from a benign pleomorphic adenoma of the parotid gland of a 61-year-old woman. They were characterized by immunofluorescence, classical cytogenetics, p53 gene mutational analysis, fluorescence in-situ hybridization, and histopathological and immunohistochemical examinations of their xenografts, to demonstrate their potency of secondary transformation. We established and characterized five cell systems (designated as SM-AP1 to SM-AP5) from a benign pleomorphic adenoma of the parotid gland. SM-AP1 to SM-AP3 showed polygonal cell shapes while SM-AP4 and SM-AP5 were spindle-shaped. SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. Chromosome analyses showed numeral abnormalities such as 5n ploidies and various kinds of structural abnormalities, such as deletions, translocations, derivatives and isodicentric chromosomes. Among them, der(9)t(9;13)(p13.3;q12.3) was shared by all five of the cell systems. In addition, they all had a common deletion of the last base G of codon 249 (AGG to AG-) of the p53 gene, which resulted in generation of its nonsense gene product. Transplanted cells in nude mice formed subcutaneous tumors, which had histological features of squamous cell carcinoma with apparent keratinizing tendencies. In addition, they had ductal arrangements or plasmacytoid appearances of tumor cells and myxoid or hyaline stromata, indicating some characteristics of pleomorphic adenoma

  15. Demonstration of the dorsal pancreatic artery by CTA to facilitate superselective arterial infusion of stem cells into the pancreas

    International Nuclear Information System (INIS)

    Purpose: To investigate the diagnostic performance of 64-section CTA in the detection of dorsal pancreatic artery before interventional therapy for patients with diabetes. Materials and methods: The study was approved by the institutional ethics committee; written informed consent was obtained. Forty-two consecutive patients with diabetes received an experimental treatment of autologous bone marrow-derived stem cell transplantation by means of infusion into the dorsal pancreatic artery. All cases underwent abdominal CTA before angiography of pancreatic arteries in order to locate the origin and course of dorsal pancreatic artery. Angiography of coeliac artery, splenic artery, common hepatic artery and superior mesenteric artery were performed both in CTA and DSA. Superselective catheterization of dorsal pancreatic artery was carried out for the infusion of stem cell. Sensitivity, specificity and accuracy for the detection of dorsal pancreatic artery with CTA were calculated using DSA images as the reference standard. Results: Thirty-five and thirty-six dorsal pancreatic arteries were detected by CTA and DSA respectively. Dorsal pancreatic artery was not visualized in either CTA or DSA in 5 patients. The sensitivity, specificity and accuracy for CTA were 94.4%, 83.3% and 92.9%. Conclusion: 64-section CTA is accurate for the detection of dorsal pancreatic artery. It may be useful for the facilitation of superselective arterial infusion of stem cells to pancreas.

  16. Simulated Coal-Gas-Fueled Molten Carbonate Fuel Cell Development Program. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1992-08-01

    This final report summarizes the technical work performed under Department of Energy Contract DE-AC21-91MC27393, ``Simulated Coal- Gas-Fueled Molten Carbonate Fuel Cell Development Program.`` This work consists of five major tasks and their respective subtasks as listed below. A brief description of each task is also provided. The Stack Design Requirements task focused on requirements and specification for designing, constructing, and testing a nominal 100-kilowatt integrated stack and on requirements for the balance-of-plant equipment to support a 1000-kilowatt integrated stack demonstrator. The Stack Design Preparation task focused on the mechanical design of a 100-kilowatt stack comprised of 8-ft{sup 2} cells incorporating the new cell configuration and component technology improvements developed in the previous DOE MCFC contract. Electrode Casting focused on developing a faster drying solvent for use in the electrode tape casting process. Electrode Heat Treatment was directed at scaling up the laboratory continuous debinding process to a new full-size IFC debinding oven coupled to a continuous belt furnace that will both debind and sinter the electrodes in one continuous process train. Repeat Part Quality Assurance and Testing provided the appropriate effort to ensure consistent, high-quality, reproducible and comparable repeat parts.

  17. Simulated Coal-Gas-Fueled Molten Carbonate Fuel Cell Development Program

    Energy Technology Data Exchange (ETDEWEB)

    1992-08-01

    This final report summarizes the technical work performed under Department of Energy Contract DE-AC21-91MC27393, Simulated Coal- Gas-Fueled Molten Carbonate Fuel Cell Development Program.'' This work consists of five major tasks and their respective subtasks as listed below. A brief description of each task is also provided. The Stack Design Requirements task focused on requirements and specification for designing, constructing, and testing a nominal 100-kilowatt integrated stack and on requirements for the balance-of-plant equipment to support a 1000-kilowatt integrated stack demonstrator. The Stack Design Preparation task focused on the mechanical design of a 100-kilowatt stack comprised of 8-ft[sup 2] cells incorporating the new cell configuration and component technology improvements developed in the previous DOE MCFC contract. Electrode Casting focused on developing a faster drying solvent for use in the electrode tape casting process. Electrode Heat Treatment was directed at scaling up the laboratory continuous debinding process to a new full-size IFC debinding oven coupled to a continuous belt furnace that will both debind and sinter the electrodes in one continuous process train. Repeat Part Quality Assurance and Testing provided the appropriate effort to ensure consistent, high-quality, reproducible and comparable repeat parts.

  18. Reactive oxygen species do not contribute to ObgE*-mediated programmed cell death.

    Science.gov (United States)

    Dewachter, Liselot; Herpels, Pauline; Verstraeten, Natalie; Fauvart, Maarten; Michiels, Jan

    2016-01-01

    Programmed cell death (PCD) in bacteria is considered an important target for developing novel antimicrobials. Development of PCD-specific therapies requires a deeper understanding of what drives this process. We recently discovered a new mode of PCD in Escherichia coli that is triggered by expression of a mutant isoform of the essential ObgE protein, ObgE*. Our previous findings demonstrate that ObgE*-mediated cell death shares key characteristics with apoptosis in eukaryotic cells. It is well-known that reactive oxygen species (ROS) are formed during PCD in eukaryotes and play a pivotal role as signaling molecules in the progression of apoptosis. Therefore, we explored a possible role for ROS in bacterial killing by ObgE*. Using fluorescent probes and genetic reporters, we found that expression of ObgE* induces formation of ROS. Neutralizing ROS by chemical scavenging or by overproduction of ROS-neutralizing enzymes did not influence toxicity of ObgE*. Moreover, expression of ObgE* under anaerobic conditions proved to be as detrimental to bacterial viability as expression under aerobic conditions. In conclusion, ROS are byproducts of ObgE* expression that do not play a role in the execution or progression of ObgE*-mediated PCD. Targeted therapies should therefore look to exploit other aspects of ObgE*-mediated PCD. PMID:27641546

  19. On-Orbit Demonstration Of Thin-Film Multi-Junction Solar Cells And Lithium-Ion Capacitors As Bus Components

    Science.gov (United States)

    Kukita, Akio; Takahashi, Masato; Shimazaki, Kazunori; Toyota, Hiroyuki; Imaizumi, Mitsuru; Kobayashi, Yuki; Takamoto, Tatsuya; Uno, Masatoshi; Shimada, Takanobu

    2011-10-01

    This paper describes an on-orbit demonstration plan for a lightweight solar panel using thin-film multi-junction (MJ) solar cells and aluminum-laminated lithium-ion capacitors (LICs). Thin-film MJ solar cells such as inverted metamorphic InGaP/GaAs/InGaAs 3J cells have flexibility as well as conversion efficiencies superior to conventional rigid 3J solar cells. A substantial reduction of satellite mass is achieved by the combination of thin-film MJ solar cells and light flexible paddles. An LIC is a hybrid-type capacitor that uses activated carbon as the cathode and carbon material pre-doped with lithium ion as the anode. LICs can be rapidly charged and discharged, and can operate in a wide temperature range for long periods. LICs are therefore suitable for long-term missions such as planetary explorations. Although these devices are very promising, so far there has been no opportunity to demonstrate their use in orbit. A lightweight thin solar panel with thin-film MJ solar cells will be installed on the Small Scientific Satellite Platform for Rapid Investigation and Test-A (SPRINT-A) satellite, which will be launched on the Epsilon launch vehicle in 2013. Utilizing the capacitor-like voltage behavior of LICs, we will employ a simple constant-power charging circuit without feedback control.

  20. Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death.

    Science.gov (United States)

    Darshan, N; Manonmani, H K

    2016-12-01

    The antimicrobial activity of prodigiosin from Serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The mode of action of prodigiosin was studied. Prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of Programmed cell death (PCD). PCD involves DNA fragmentation, generation of ROS, and expression of a protein with caspase-like substrate specificity in bacterial cells. Prodigiosin was observed to be internalized into bacterial cells and was localized predominantly in the membrane and the nuclear fraction, thus, facilitating intracellular trafficking and then binding of prodigiosin to the bacterial DNA. Corresponding to an increasing concentration of prodigiosin, the level of certain proteases were observed to increase in bacteria studied, thus initiating the onset of PCD. Prodigiosin at a sub-inhibitory concentration inhibits motility of pathogens. Our observations indicated that prodigiosin could be a promising antibacterial agent and could be used in the prevention of bacterial infections. PMID:27460563

  1. Interplay between autophagy and programmed cell death in mammalian neural stem cells

    Directory of Open Access Journals (Sweden)

    Kyung Min Chung

    2013-08-01

    Full Text Available Mammalian neural stem cells (NSCs are of particular interestbecause of their role in brain development and function. Recentfindings suggest the intimate involvement of programmed celldeath (PCD in the turnover of NSCs. However, the underlyingmechanisms of PCD are largely unknown. Although apoptosis isthe best-defined form of PCD, accumulating evidence hasrevealed a wide spectrum of PCD encompassing apoptosis,autophagic cell death (ACD and necrosis. This mini-reviewaims to illustrate a unique regulation of PCD in NSCs. Theresults of our recent studies on autophagic death of adulthippocampal neural stem (HCN cells are also discussed. HCNcell death following insulin withdrawal clearly provides areliable model that can be used to analyze the molecularmechanisms of ACD in the larger context of PCD. Moreresearch efforts are needed to increase our understanding of themolecular basis of NSC turnover under degenerating conditions,such as aging, stress and neurological diseases. Efforts aimed atprotecting and harnessing endogenous NSCs will offer novelopportunities for the development of new therapeutic strategiesfor neuropathologies. [BMB Reports 2013; 46(8: 383-390

  2. Salt Stress-induced Programmed Cell Death in Rice Root Tip Cells

    Institute of Scientific and Technical Information of China (English)

    Jian-You Li; Ai-Liang Jiang; Wei Zhang

    2007-01-01

    Salt stressed rice root tips were used to investigate the changes of reactive oxygen species (ROS) and antioxidant enzymes at the early stages of programmed cell death (PCD). The results indicated that 500 mmol/L NaCl treatment could lead to specific features of PCD in root tips, such as DNA ladder, nuclear condense and deformation, and transferase mediated dUTP nick end labeling positive reaction, which were initiated at 4 h of treatment and progressed thereafter. Cytochrome c release from mitochondria into cytoplasm was also observed, which occurred at 2 h and was earlier than the above nuclear events. In the very early phase of PCD, an immediate burst in hydrogen peroxide and superoxide anion production rate was accompanied by two-phase changes of superoxide dismutases and ascorbate peroxidase. A short period of increase in the activity was followed by prolonged impairment. Thus,we conclude that salt can induce PCD in rice root tip cells, and propose that in the early phase of rice root tip cell PCD, salt stress-induced oxidative burst increased the antioxidant enzyme activity, which, In turn, scavenged the ROS and abrogated PCD. Also, when the stress is prolonged, the antioxidant system is damaged and accumulated ROS induces the PCD process, which leads to cytochrome c release and nuclear change.

  3. Photoacoustic spectral analysis to sense programmed erythrocyte cell death (eryptosis) for monitoring cancer response to treatment

    Science.gov (United States)

    Fadhel, Muhannad N.; Kibria, Fayruz; Kolios, Michael C.

    2016-03-01

    Many types of cancer therapies target the tumor microenvironment, causing biochemical and morphological changes in tissues. In therapies using ultrasound activated microbubbles, vascular collapse is typically reported. Red blood cells (RBCs) that leak out of the vasculature become exposed to the ceramide that is released from damaged endothelial cells. Ceramide can induce programmed cell death in RBCs (eryptosis), and is characterized by cell shrinkage, membrane blebbing and scrambling. Since the effect of eryptotic cells on generated photoacoustics (PA) signals has not been reported, we investigated the potential PA may have for cancer treatment monitoring by using PA spectral analysis to sense eryptosis. To induce eryptosis, C2-ceramide was added to RBC suspensions and that were incubated for 24 hours at 37°C. A control and ceramide-induced sample was imaged in a vessel phantom using a high frequency PA system (VevoLAZR, 10 - 45 MHz bandwidth) irradiated with multiple wavelengths ranging from 680 to 900 nm. PA spectral parameters were measured and linked to changes in RBCs as it underwent eryptosis. These samples were examined using optical microscopy, a blood gas analyzer and an integrating sphere setup to measure optical properties (wavelengths 600 - 900 nm). The results of the experiment demonstrate how PA spectral analysis can be used to identify eryptosis at a depth of more than 1 cm into the phantom using ultrasound derived the y-intercept and mid bandfit (MBF) parameters at optical wavelengths of 800 - 900 nm. These parameters were correlated to the morphological and biochemical changes that eryptotic RBCs display. The results establish the potential of PA in cancer treatment monitoring through sensing treatment induced eryptosis.

  4. The development of a state-of-the-art experimental setup demonstrated by the investigation of fuel cell reactions in alkaline electrolyte

    Energy Technology Data Exchange (ETDEWEB)

    Wiberg, Gustav Karl Henrik

    2010-10-04

    The objectives of this work can be separated into three different topics: the design and development of a state-of-the art electrochemical experiment setup, which is then followed by two separate experimental studies in alkaline electrolyte. These studies demonstrate the capabilities of the experimental setup, and each focus on separate model catalysts. The first study investigated the influence of Pt oxide formation on the measured catalytic activity of FC relevant reactions on polycrystalline Pt in alkaline electrolyte. The second study focused on the characterisation of the ORR for a non-platinum catalyst, in this case Ag, by adapting the established thin-film RDE methodology employed for characterising Pt based electrocatalysts. A state-of-the-art electrochemical experimental setup comprises of a largely automated setup that allows meticulous control over experimental parameters such as potential, temperature, purging gas and solution convection. In order to realise such a setup, both experimental hardware and software were developed. In particular, a custom built analogue potentiostat optimised for single working electrode measurements was constructed. The potentiostat features R{sub sol}-compensation which can be monitored online due to its fully analogue design, allowing the precise current and potential relationship to be measured. In addition, the experimental throughput was enhanced by fabricating a modular add-on device, the MWE, which allows simultaneous electrochemical measurement on up to 8 parallel working electrodes. The MWE device is compatible with any single channel potentiostat, enhancing existing instrumentation. Several Teflon cells were designed for electrochemical investigations in acid and alkaline electrolytes, and were adapted to work using either the RDE or MWE. A gas changer was also assembled, which enabled computer controlled switching of electrolyte purge gas. Furthermore, in order to control the potentiostat and the accessory

  5. Demonstration of an Electrochemical Liquid Cell for Operando Transmission Electron Microscopy Observation of the Lithiation/Delithiation Behavior of Si Nanowire Battery Anodes

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Meng [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Parent, Lucas R. [Univ. of California, Davis, CA (United States); Mehdi, Beata L. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Unocic, Raymond R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McDowell, Matthew T. [Stanford Univ., CA (United States); Sacci, Robert L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Xu, Wu [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Connell, Justin G. [Northwestern Univ., Evanston, IL (United States); Xu, Pinghong [Univ. of California, Davis, CA (United States); Abellan Baeza, Patricia [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Chen, Xilin [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhang, Yaohui [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Harbin Inst. of Technology (China); Perea, Daniel E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Evans, James E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lauhon, Lincoln [Northwestern Univ., Evanston, IL (United States); Zhang, Jiguang [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Liu, Jun [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Browning, Nigel D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Cui, Yi [Stanford Univ., CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States); Arslan, Ilke [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Wang, Chong M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2013-11-13

    Over the last few years, in-situ transmission electron microscopy (TEM) studies of lithium ion batteries using an open-cell configuration have helped us to gain fundamental insights into the structural and chemical evolution of the electrode materials in real time. In the standard open-cell configuration, the electrolyte is either solid lithium oxide or an ionic liquid, which is point-contacted with the electrode. This cell design is inherently different from a real battery, where liquid electrolyte forms conformal contact with electrode materials. The knowledge learnt from open cells can deviate significantly from the real battery, calling for operando TEM technique with conformal liquid electrolyte contact. In this paper, we developed an operando TEM electrochemical liquid cell to meet this need, providing the configuration of a real battery and in a relevant liquid electrolyte. To demonstrate this novel technique, we studied the lithiation/delithiation behavior of single Si nanowires. Some of lithiation/delithation behaviors of Si obtained using the liquid-cell are consistent with the results from the open-cell studies. However, we also discovered new insights different from the open cell configuration - the dynamics of the electrolyte and, potentially, a future quantitative characterization of the SEI layer formation and structural and chemical evolution.

  6. Aged Muscle Demonstrates Fiber-Type Adaptations in Response to Mechanical Overload, in the Absence of Myofiber Hypertrophy, Independent of Satellite Cell Abundance.

    Science.gov (United States)

    Lee, Jonah D; Fry, Christopher S; Mula, Jyothi; Kirby, Tyler J; Jackson, Janna R; Liu, Fujun; Yang, Lin; Dupont-Versteegden, Esther E; McCarthy, John J; Peterson, Charlotte A

    2016-04-01

    Although sarcopenia, age-associated loss of muscle mass and strength, is neither accelerated nor exacerbated by depletion of muscle stem cells, satellite cells, we hypothesized that adaptation in sarcopenic muscle would be compromised. To test this hypothesis, we depleted satellite cells with tamoxifen treatment of Pax7(CreER)-DTA mice at 4 months of age, and 20 months later subjected the plantaris muscle to 2 weeks of mechanical overload. We found myofiber hypertrophy was impaired in aged mice regardless of satellite cell content. Even in the absence of growth, vehicle-treated mice mounted a regenerative response, not apparent in tamoxifen-treated mice. Further, myonuclear accretion occurred in the absence of growth, which was prevented by satellite cell depletion, demonstrating that myonuclear addition is insufficient to drive myofiber hypertrophy. Satellite cell depletion increased extracellular matrix content of aged muscle that was exacerbated by overload, potentially limiting myofiber growth. These results support the idea that satellite cells regulate the muscle environment, and that their loss during aging may contribute to fibrosis, particularly during periods of remodeling. Overload induced a fiber-type composition improvement, independent of satellite cells, suggesting that aged muscle is very responsive to exercise-induced enhancement in oxidative capacity, even with an impaired hypertrophic response. PMID:25878030

  7. TNX GeoSiphon Cell (TGSC-1) Phase II Minimum Flushing Velocity Deployment/Demonstration Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Phifer, M.A.

    1999-10-25

    The TNX Area is a semi-works facility for the Savannah River Technology Center (SRTC), which is located one-quarter mile from the Savannah river at the Savannah River Site. As the result of TNX operation, groundwater contamination has occurred. The predominant contaminants detected in the flood plain downgradient from TNX are trichloroethylene (TCE) and nitrate.Treatability studies into the applicability of a groundwater remediation system combining GeoSiphon Cell and zero-valent iron technologies for treatment of the TCE-contaminated groundwater at TNX have been conducted. These treatability studies have been conducted by SRTC under the sponsorship of the Environmental Restoration Department.

  8. Focal Adhesion Kinase Inhibitors in Combination with Erlotinib Demonstrate Enhanced Anti-Tumor Activity in Non-Small Cell Lung Cancer.

    Directory of Open Access Journals (Sweden)

    Grant A Howe

    Full Text Available Blockade of epidermal growth factor receptor (EGFR activity has been a primary therapeutic target for non-small cell lung cancers (NSCLC. As patients with wild-type EGFR have demonstrated only modest benefit from EGFR tyrosine kinase inhibitors (TKIs, there is a need for additional therapeutic approaches in patients with wild-type EGFR. As a key component of downstream integrin signalling and known receptor cross-talk with EGFR, we hypothesized that targeting focal adhesion kinase (FAK activity, which has also been shown to correlate with aggressive stage in NSCLC, would lead to enhanced activity of EGFR TKIs. As such, EGFR TKI-resistant NSCLC cells (A549, H1299, H1975 were treated with the EGFR TKI erlotinib and FAK inhibitors (PF-573,228 or PF-562,271 both as single agents and in combination. We determined cell viability, apoptosis and 3-dimensional growth in vitro and assessed tumor growth in vivo. Treatment of EGFR TKI-resistant NSCLC cells with FAK inhibitor alone effectively inhibited cell viability in all cell lines tested; however, its use in combination with the EGFR TKI erlotinib was more effective at reducing cell viability than either treatment alone when tested in both 2- and 3-dimensional assays in vitro, with enhanced benefit seen in A549 cells. This increased efficacy may be due in part to the observed inhibition of Akt phosphorylation when the drugs were used in combination, where again A549 cells demonstrated the most inhibition following treatment with the drug combination. Combining erlotinib with FAK inhibitor was also potent in vivo as evidenced by reduced tumor growth in the A549 mouse xenograft model. We further ascertained that the enhanced sensitivity was irrespective of the LKB1 mutational status. In summary, we demonstrate the effectiveness of combining erlotinib and FAK inhibitors for use in known EGFR wild-type, EGFR TKI resistant cells, with the potential that a subset of cell types, which includes A549, could be

  9. A hot-electron thermophotonic solar cell demonstrated by thermal up-conversion of sub-bandgap photons

    Science.gov (United States)

    Farrell, Daniel J.; Sodabanlu, Hassanet; Wang, Yunpeng; Sugiyama, Masakazu; Okada, Yoshitaka

    2015-01-01

    The direct conversion of solar energy to electricity can be broadly separated into two main categories: photovoltaics and thermal photovoltaics, where the former utilizes gradients in electrical potential and the latter thermal gradients. Conventional thermal photovoltaics has a high theoretical efficiency limit (84%) but in practice cannot be easily miniaturized and is limited by the engineering challenges of sustaining large (>1,000 K) temperature gradients. Here we show a hot-carrier-based thermophotonic solar cell, which combines the compact nature of photovoltaic devices with the potential to reach the high-efficiency regime of thermal photovoltaics. In the device, a thermal gradient of 500 K is established by hot electrons, under Stokes illumination, rather than by raising the temperature of the material itself. Under anti-Stokes (sub-bandgap) illumination we observe a thermal gradient of ∼20 K, which is maintained by steady-state Auger heating of carriers and corresponds to a internal thermal up-conversion efficiency of 30% between the collector and solar cell. PMID:26541415

  10. Education Payload Operation - Demonstrations

    Science.gov (United States)

    Keil, Matthew

    2009-01-01

    Education Payload Operation - Demonstrations (EPO-Demos) are recorded video education demonstrations performed on the International Space Station (ISS) by crewmembers using hardware already onboard the ISS. EPO-Demos are videotaped, edited, and used to enhance existing NASA education resources and programs for educators and students in grades K-12. EPO-Demos are designed to support the NASA mission to inspire the next generation of explorers.

  11. Safety demonstration tests of hypothetical explosive burning in the cell and air ventilation system in a nuclear fuel reprocessing plant

    International Nuclear Information System (INIS)

    This paper reports on a nuclear fuel reprocessing plant equipped with an air ventilation system consisting of cells, ducts, dampers, high-efficiency particulate air filters, and blowers. This ventilation system is required to have multiple safeguards in order to confine airborne radioactive materials within the plant in the event of fire, explosion, and criticality. To evaluate these safeguards, three kinds of explosive burning tests are performed using a large-scale facility simulating the ventilation system of a reprocessing plant. In the boilover test, an organic solvent is burned on a layer of water in a burning pan to determine the magnitude of the burning caused by the sudden boiling of the water under the solvent. The optimum conditions for boilover burning are determined by the relationship between the pan size and the ventilation rate

  12. Costimulation of CD3/TcR complex with either integrin or nonintegrin ligands protects CD4+ allergen-specific T-cell clones from programmed cell death.

    Science.gov (United States)

    Agea, E; Bistoni, O; Bini, P; Migliorati, G; Nicoletti, I; Bassotti, G; Riccardi, C; Bertotto, A; Spinozzi, F

    1995-08-01

    An optimal stimulation of CD4+ cells in an immune response requires not only signals transduced via the TcR/CD3 complex, but also costimulatory signals delivered as a consequence of interactions between T-cell surface-associated costimulatory receptors and their counterparts on antigen-presenting cells (APC). The intercellular adhesion molecule-1 (ICAM-1, CD54) efficiently costimulates proliferation of resting, but not antigen-specific, T cells. In contrast, CD28 and CD2 support interleukin (IL)-2 synthesis and proliferation of antigen-specific T cells more efficiently than those of resting T cells. The molecular basis for this differential costimulation of T cells is poorly understood. Cypress-specific T-cell clones (TCC) were generated from four allergic subjects during in vivo seasonal exposure to the allergen. Purified cypress extract was produced directly from fresh collected pollen and incubated with the patients' mononuclear cells. Repeated allergen stimulation was performed in T-cell cultures supplemented with purified extract and autologous APC. The limiting-dilution technique was then adopted to generate allergen-specific TCC, which were also characterized by their cytokine secretion pattern as Th0 (IL-4 plus interferon-gamma) or Th2 (IL-4). Costimulation-induced proliferation or apoptosis was measured by propidium iodide cytofluorometric assay. By cross-linking cypress-specific CD4+ and CD8+ T-cell clones with either anti-CD3 or anti-CD2, anti-CD28, and anti-CD54 monoclonal antibodies, we demonstrated that CD4+ clones (with Th0- or Th2-type cytokine production pattern) undergo programmed cell death only after anti-CD3 stimulation, whereas costimulation with either anti-CD54 or anti-CD28 protects target cells from apoptosis.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7503404

  13. Both the caspase CSP-1 and a caspase-independent pathway promote programmed cell death in parallel to the canonical pathway for apoptosis in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Daniel P Denning

    Full Text Available Caspases are cysteine proteases that can drive apoptosis in metazoans and have critical functions in the elimination of cells during development, the maintenance of tissue homeostasis, and responses to cellular damage. Although a growing body of research suggests that programmed cell death can occur in the absence of caspases, mammalian studies of caspase-independent apoptosis are confounded by the existence of at least seven caspase homologs that can function redundantly to promote cell death. Caspase-independent programmed cell death is also thought to occur in the invertebrate nematode Caenorhabditis elegans. The C. elegans genome contains four caspase genes (ced-3, csp-1, csp-2, and csp-3, of which only ced-3 has been demonstrated to promote apoptosis. Here, we show that CSP-1 is a pro-apoptotic caspase that promotes programmed cell death in a subset of cells fated to die during C. elegans embryogenesis. csp-1 is expressed robustly in late pachytene nuclei of the germline and is required maternally for its role in embryonic programmed cell deaths. Unlike CED-3, CSP-1 is not regulated by the APAF-1 homolog CED-4 or the BCL-2 homolog CED-9, revealing that csp-1 functions independently of the canonical genetic pathway for apoptosis. Previously we demonstrated that embryos lacking all four caspases can eliminate cells through an extrusion mechanism and that these cells are apoptotic. Extruded cells differ from cells that normally undergo programmed cell death not only by being extruded but also by not being engulfed by neighboring cells. In this study, we identify in csp-3; csp-1; csp-2 ced-3 quadruple mutants apoptotic cell corpses that fully resemble wild-type cell corpses: these caspase-deficient cell corpses are morphologically apoptotic, are not extruded, and are internalized by engulfing cells. We conclude that both caspase-dependent and caspase-independent pathways promote apoptotic programmed cell death and the phagocytosis of cell

  14. Involvement of ethylene and lipid signalling in cadmium-induced programmed cell death in tomato suspension cells.

    Science.gov (United States)

    Yakimova, E T; Kapchina-Toteva, V M; Laarhoven, L-J; Harren, F M; Woltering, E J

    2006-10-01

    Cadmium-induced cell death was studied in suspension-cultured tomato (Lycopersicon esculentum Mill.) cells (line MsK8) treated with CdSO(4). Within 24 h, cadmium treatment induced cell death in a concentration-dependent manner. Cell cultures showed recovery after 2-3 days which indicates the existence of an adaptation mechanism. Cadmium-induced cell death was alleviated by the addition of sub muM concentrations of peptide inhibitors specific to human caspases indicating that cell death proceeds through a mechanism with similarities to animal programmed cell death (PCD, apoptosis). Cadmium-induced cell death was accompanied by an increased production of hydrogen peroxide (H(2)O(2)) and simultaneous addition of antioxidants greatly reduced cell death. Inhibitors of phospholipase C (PLC) and phospholipase D (PLD) signalling pathway intermediates reduced cadmium-induced cell death. Treatment with the G-protein activator mastoparan and a cell permeable analogue of the lipid signal second messenger phosphatidic acid (PA) induced cell death. Ethylene, while not inducing cell death when applied alone, stimulated cadmium-induced cell death. Application of the ethylene biosynthesis inhibitor aminoethoxy vinylglycine (AVG) reduced cadmium-induced cell death, and this effect was alleviated by simultaneous treatment with ethylene. Together the results show that cadmium induces PCD exhibiting apoptotic-like features. The cell death process requires increased H(2)O(2) production and activation of PLC, PLD and ethylene signalling pathways.

  15. Differential programming of B cells in AID deficient mice.

    Directory of Open Access Journals (Sweden)

    Marc A Hogenbirk

    Full Text Available The Aicda locus encodes the activation induced cytidine deaminase (AID and is highly expressed in germinal center (GC B cells to initiate somatic hypermutation (SHM and class switch recombination (CSR of immunoglobulin (Ig genes. Besides these Ig specific activities in B cells, AID has been implicated in active DNA demethylation in non-B cell systems. We here determined a potential role of AID as an epigenetic eraser and transcriptional regulator in B cells. RNA-Seq on different B cell subsets revealed that Aicda(-/- B cells are developmentally affected. However as shown by RNA-Seq, MethylCap-Seq, and SNP analysis these transcriptome alterations may not relate to AID, but alternatively to a CBA mouse strain derived region around the targeted Aicda locus. These unexpected confounding parameters provide alternative, AID-independent interpretations on genotype-phenotype correlations previously reported in numerous studies on AID using the Aicda(-/- mouse strain.

  16. DoD Climate Change Fuel Cell Program

    Energy Technology Data Exchange (ETDEWEB)

    Steven A. Gabrielle

    2007-04-30

    A grant was awarded to PPL EnergyPlus, LLC for two (2) 250kW Molten Carbonate Fuel Cells at Pepperidge Farm, Inc. on 9/30/03. Pepperidge Farm subsequently signed a contract for one 250kW fuel cell. A request was made and granted to apply the award for the second fuel cell to the Sheraton New York Hotel & Towers (see attached email). This report discusses the first year of operation of a fuel cell power plant located at Pepperidge Farm, Inc., Bloomfield, Connecticut and a fuel cell power plant located at Sheraton New York Hotel & Towers, New York, New York. PPL EnergyPlus, LLC installed the plants under a contract with Pepperidge Farm and Starwood Hotels & Resorts Worldwide, Inc. Two DFC 300 fuel cells, manufactured by FuelCell Energy, Inc. of Danbury, CT were selected for the project. The fuel cell located at Pepperidge Farm successfully operated from January 16, 2006 to January 15, 2007. The fuel cell located at Sheraton New York Hotel & Tower successfully operated from May 19, 2005 to May 18, 2006.This report discusses the performance of these plants during these periods.

  17. A gibberellin-induced nuclease is localized in the nucleus of wheat aleurone cells undergoing programmed cell death

    OpenAIRE

    Domínguez, Fernándo; Moreno Onorato, Francisco Javier; Cejudo Fernández, Francisco Javier

    2003-01-01

    The aleurone layer of cereal grains undergoes a gibberellin-regulated process of programmed cell death (PCD) following germination. We have applied a combination of ultrastructural and biochemical approaches to analyze aleurone PCD in intact wheat grains. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay revealed that PCD was initiated in aleurone cells proximal to the embryo and then extended to distal cells. DNA fragmentation and terminal deoxynucleotidyl trans...

  18. Deep Sequencing of the T-cell Receptor Repertoire Demonstrates Polyclonal T-cell Infiltrates in Psoriasis [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Jamie L. Harden

    2015-08-01

    Full Text Available It is well known that infiltration of pathogenic T-cells plays an important role in psoriasis pathogenesis. However, the antigen specificity of these activated T-cells is relatively unknown. Previous studies using T-cell receptor polymerase chain reaction technology (TCR-PCR have suggested there are expanded T-cell receptor (TCR clones in psoriatic skin, suggesting a response to an unknown psoriatic antigen. Here we describe the results of high-throughput deep sequencing of the entire αβ- and γδ- TCR repertoire in normal healthy skin and psoriatic lesional and non-lesional skin. From this study, we were able to determine that there is a significant increase in the abundance of unique β- and γ- TCR sequences in psoriatic lesional skin compared to non-lesional and normal skin, and that the entire T-cell repertoire in psoriasis is polyclonal, with similar diversity to normal and non-lesional skin. Comparison of the αβ- and γδ- TCR repertoire in paired non-lesional and lesional samples showed many common clones within a patient, and these close were often equally abundant in non-lesional and lesional skin, again suggesting a diverse T-cell repertoire. Although there were similar (and low amounts of shared β-chain sequences between different patient samples, there was significantly increased sequence sharing of the γ-chain in psoriatic skin from different individuals compared to those without psoriasis. This suggests that although the T-cell response in psoriasis is highly polyclonal, particular γδ- T-cell subsets may be associated with this disease. Overall, our findings present the feasibility of this technology to determine the entire αβ- and γδ- T-cell repertoire in skin, and that psoriasis contains polyclonal and diverse αβ- and γδ- T-cell populations.

  19. Design of virtual manufacturing cells : a mathematical programming approach

    NARCIS (Netherlands)

    Slomp, J.; Chowdary, B.V.; Suresh, N.

    2005-01-01

    In this paper, a new type of virtual cellular manufacturing (CM) system is considered, and a multi-objective design procedure is developed for designing such cells in real time. Retaining the functional layout, virtual cells are addressed as temporary groupings of machines, jobs and workers to reali

  20. Tetracycline regulator expression alters the transcriptional program of mammalian cells

    OpenAIRE

    Hackl, Hubert; Rommer, Anna; Konrad, Torsten A; Nassimbeni, Christine; Wieser, Rotraud

    2010-01-01

    Tetracycline regulated ectopic gene expression is a widely used tool to study gene function. However, the tetracycline regulator (tetR) itself has been reported to cause certain phenotypic changes in mammalian cells. We, therefore, asked whether human myeloid U937 cells expressing the tetR in an autoregulated manner would exhibit alterations in gene expression upon removal of tetracycline.