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Sample records for cea antigen

  1. Reagents for radioimmunological determination of carcinoembryonic antigen (CEA)

    International Nuclear Information System (INIS)

    The work was undertaken to prepare the reagents for carcinoembryonic antigen (CEA) radioimmunoassay with double antibody method. The CEA standard of high immunoreactivity was prepared and purified. The purified CEA was used for immunozation of goats. The goat anti - CEA sera were received. IgG fraction from normal goat serum was purified and used for the production of horse anti-goat IgG serum which was then used in the radioimmunoassay of CEA. The labelling of CEA with iodine-125 has been carried out be means of the enzymatic method.(Z.R.)

  2. A recombinant vaccinia virus expressing human carcinoembryonic antigen (CEA).

    Science.gov (United States)

    Kaufman, H; Schlom, J; Kantor, J

    1991-07-30

    Carcinoembryonic antigen (CEA) is a 180-kDa glycoprotein expressed on most gastrointestinal carcinomas. A 2.4-kb cDNA clone, containing the complete coding sequence, was isolated from a human colon tumor cell library and inserted into a vaccinia virus genome. This newly developed construct was characterized by Southern blotting, DNA hybridization studies, and polymerase chain reaction analysis. The CEA gene was stably integrated into the vaccinia virus thymidine kinase gene. The recombinant was efficiently replicated upon serial passages in cell cultures and in animals. The recombinant virus expresses on the surface of infected cells a protein product recognized by a monoclonal antibody (COL-I) directed against CEA. Immunization of mice with the vaccinia construct elicited a humoral immune response against CEA. Pilot studies also showed that administration of the recombinant CEA vaccinia construct was able to greatly reduce the growth in mice of a syngeneic murine colon adenocarcinoma which had been transduced with the human CEA gene. The use of this new recombinant CEA vaccinia construct may thus provide an approach in the specific active immunotherapy of human GI cancer and other CEA expressing carcinoma types.

  3. Carcinoembryonic antigen (CEA) dynamics in stomach cancer patients receiving cryotherapy

    International Nuclear Information System (INIS)

    Radioimmunologic assays of blood serum carcinoembryonic antigen (CEA) level were conducted at major stages of treatment of gastric cancer by subtotal stomach resection and gastrectomy with preliminary cryotreatment and thawing of tumor. A short-term rise in CEA level occurred in 53.9 % of cases 3-4 days after combined therapy. A decrease in CEA concentration at discharge from hospital as compared with preoperative level and that registered 3-4 days after operation was observed in 50 and 75 % of cases of combined therapy, respectively, and 47.5 and 37.5 % of controls (surgery without cryotreatment). There was nocorrelation between cryotreatment and changes in CEA level in gastric ulcer patients

  4. Carcinoembryonic antigen (CEA) level, CEA ratio, and treatment outcome of rectal cancer patients receiving pre-operative chemoradiation and surgery

    International Nuclear Information System (INIS)

    To investigate serum carcinoembryonic antigen (CEA) as a prognostic factor for rectal cancer patients receiving pre-operative chemoradiotherapy (CRT). Between 2000 and 2009, 138 patients with advanced rectal cancer receiving CRT before surgery at our hospital were retrospectively classified into 3 groups: pre-CRT CEA <6 ng/ml (group L; n = 87); pre-CRT CEA ≥ 6 ng/ml and post-CRT CEA <6 ng/ml (group H-L; n = 32); and both pre- and post-CRT CEA ≥ 6 ng/ml (group H-H; n = 19). CEA ratio (defined as post-CRT CEA divided by pre-CRT CEA), post-CRT CEA level and other factors were reviewed for prediction of pathologic complete response (pCR). Five-year disease-free survival (DFS) was better in groups L (69.0%) and H-L (74.5%) than in group H-H (44.9%) (p = 0.024). Pathologic complete response was observed in 19.5%, 21.9% and 5.3% of groups L, H-L and H-H respectively (p = 0.281). Multivariate analysis showed that ypN stage and pCR were independent prognostic factors for DFS and that post-CRT CEA level was independently predictive of pCR. As a whole, post-CRT CEA <2.61 ng/ml predicted pCR (sensitivity 76.0%; specificity 58.4%). For those with pre-CRT CEA ≥6 ng/ml, post-CRT CEA and CEA ratio both predicted pCR (sensitivity 87.5%, specificity 76.7%). In patients with pre-CRT serum CEA ≥6 ng/ml, those with “normalized” CEA levels after CRT may have similar DFS to those with “normal” (<6 ng/ml) pre-CRT values. Post-CRT CEA level is a predictor for pCR, especially in those with pre-CRT CEA ≥6 ng/ml

  5. Carcinoembryonic antigen (CEA) as tumor marker in lung cancer

    DEFF Research Database (Denmark)

    Knudsen, Mie Grunnet; Sorensen, J B

    2012-01-01

    The use of CEA as a prognostic and predictive marker in patients with lung cancer is widely debated. The aim of this review was to evaluate the results from studies made on this subject. Using the search words "CEA", "tumor markers in lung cancer", "prognostic significance", "diagnostic...... significance" and "predictive significance", a search was carried out on PubMed. Exclusion criteria was articles never published in English, articles before 1981 and articles evaluating tumor markers in lung cancer not involving CEA. Initially 217 articles were found, and 34 were left after selecting those...... relevant for the present study. Four of these included both Non-Small Cell Lung Cancer (NSCLC) and Small Cell Lung Cancer (SCLC) patients, and 31 dealt solely with NSCLC patients. Regarding SCLC no studies showed that serum level of CEA was a prognostic marker for overall survival (OS). The use of CEA...

  6. CHARACTERIZATION OF MONOCLONAL ANTIBODY CL58 AGAINST CARCINOEMBRYONIC ANTIGEN (CEA) AND STUDY OF ITS BIODISTRIBUTION

    Institute of Scientific and Technical Information of China (English)

    李振甫; 杨志; 张宏; 顾晋

    2002-01-01

    Objective: To study the preparation and characterization of monoclonal antibody (McAb) against carcinoembryonic antigen (CEA). Methods: CEA antigen was extracted from metastasized liver of patients with colorectal cancer and used for the preparation of McAb against CEA by hybridoma technique. Immunoreactivity of McAb to CEA antigen was evaluated using ELISA. Mouse ascites was purified by two steps, high performance liquid chromatography (HPLC) using protein A and high performance hydroxylapatite (HPHT). Normal adult tissues and tumor specimen were used for immunohistochemical evaluation of the McAb. Isotope 99mTc labeled CEA McAb was used for biodistribution in tumor-bearing mouse. Results: Purified CEA antigen was a glycoprotein of 180 kD. Anti-CEA McAb affinity constant was 7.4x109/M. The McAb showed positive staining in 54-88% of colorectal cancer, gastric cancer and lung cancer, while negative for normal tissues. 24 hours after injection of 99mTc labeled McAb, tumor ID%/g was higher than 15% and tumor/blood, tumor/kidney and tumor/liver were 1.82, 1.51 and 2.92 respectively. T/NT ratios of other viscera were over 3.0. Conclusion: Purified CEA antigen had very good immunogenicity. The anti-CEA McAb was highly specific. 99mTc labeled McAb was stabled both in vivo and in vitro. In vivo distribution result was satisfactory. McAb CL58 may be useful for RII and RIGS.

  7. Intensified follow-up in colorectal cancer patients using frequent Carcino-Embryonic Antigen (CEA) measurements and CEA-triggered imaging : Results of the randomized "CEAwatch" trial

    NARCIS (Netherlands)

    Verberne, C. J.; Zhan, Z.; van den Heuvel, E.; Grossmann, I.; Doornbos, P. M.; Havenga, K.; Manusama, E.; Klaase, J.; van der Mijle, H. C. J.; Lamme, B.; Bosscha, K.; Baas, P.; van Ooijen, B.; Nieuwenhuijzen, G.; Marinelli, A.; van der Zaag, E.; Wasowicz, D.; de Bock, G. H.; Wiggers, T.

    2015-01-01

    Aim: The value of frequent Carcino-Embryonic Antigen (CEA) measurements and CEA-triggered imaging for detecting recurrent disease in colorectal cancer (CRC) patients was investigated in search for an evidence-based follow-up protocol. Methods: This is a randomized-controlled multicenter prospective

  8. Characterization of gastric adenocarcinoma cell lines established from CEA424/SV40 T antigen-transgenic mice with or without a human CEA transgene

    International Nuclear Information System (INIS)

    Gastric carcinoma is one of the most frequent cancers worldwide. Patients with gastric cancer at an advanced disease stage have a poor prognosis, due to the limited efficacy of available therapies. Therefore, the development of new therapies, like immunotherapy for the treatment of gastric cancer is of utmost importance. Since the usability of existing preclinical models for the evaluation of immunotherapies for gastric adenocarcinomas is limited, the goal of the present study was to establish murine in vivo models which allow the stepwise improvement of immunotherapies for gastric cancer. Since no murine gastric adenocarcinoma cell lines are available we established four cell lines (424GC, mGC3, mGC5, mGC8) from spontaneously developing tumors of CEA424/SV40 T antigen (CEA424/Tag) mice and three cell lines derived from double-transgenic offsprings of CEA424/Tag mice mated with human carcinoembryonic antigen (CEA)-transgenic (CEA424/Tag-CEA) mice (mGC2CEA, mGC4CEA, mGC11CEA). CEA424/Tag is a transgenic C57BL/6 mouse strain harboring the Tag under the control of a -424/-8 bp CEA gene promoter which leads to the development of invasive adenocarcinoma in the glandular stomach. Tumor cell lines established from CEA424/Tag-CEA mice express the well defined tumor antigen CEA under the control of its natural regulatory elements. The epithelial origin of the tumor cells was proven by morphological criteria including the presence of mucin within the cells and the expression of the cell adhesion molecules EpCAM and CEACAM1. All cell lines consistently express the transgenes CEA and/or Tag and MHC class I molecules leading to their susceptibility to lysis by Tag-specific CTL in vitro. Despite the presentation of CTL-epitopes derived from the transgene products the tumor cell lines were tumorigenic when grafted into C57BL/6, CEA424/Tag or CEA424/Tag-CEA-transgenic hosts and no significant differences in tumor take and tumor growth were observed in the different hosts. Although

  9. Elevated Level of Serum Carcinoembryonic Antigen (CEA) and Search for a Malignancy: A Case Report.

    Science.gov (United States)

    Asad-Ur-Rahman, Fnu; Saif, Muhammad W

    2016-01-01

    Carcinoembryonic antigen (CEA) has been shown to be associated with tumor burden in patients with colorectal cancer. However, it is also elevated to a significant degree in a number of other malignant and non-malignant conditions. We report a case of reversible CEA elevation in a patient using lithium for bipolar disorder. A 58-year-old female with a longstanding smoking history and a past medical history of chronic obstructive pulmonary disease (COPD), bipolar illness, hypothyroidism, and obesity was found to have an elevated CEA level of 11.2 ng/ml (normal level lung cancer; however, it did not reveal any evidence of malignancy. Upon review of her medications, she reported that she had recently started lithium for her bipolar illness. We followed up her CEA level while her dose of lithium was reduced from 450 to 300 mg per day. Her CEA level decreased from 25 mg/dl to 6.1 mg/dl and remained stable over the course of the next eight months. Our case is the first case report that identifies lithium as a potential cause of reversible CEA elevation. The underlying mechanism is yet to be elucidated, but it underscores the importance of investigating the medications as part of the workup. PMID:27446768

  10. Hookah smoking and cancer: carcinoembryonic antigen (CEA) levels in exclusive/ever hookah smokers

    OpenAIRE

    Chaouachi Kamal; Sajid Khan; Mahmood Rubaida

    2008-01-01

    Abstract Background We have recently published some work on CEA levels in hookah (also called narghile, shisha elsewhere) and cigarette smokers. Hookah smokers had higher levels of CEA than non-smokers although mean levels were low compared to cigarette smokers. However some of them were also users of other tobacco products (cigarettes, bidis, etc.). Objectives To find serum CEA levels in ever/exclusive hookah smokers, i.e. those who smoked only hookah (no cigarettes, bidis, etc.), prepared b...

  11. Localization of 131Ilabeled goat and primate anti-carcinoembryonic antigen(CEA) antibodies in patients with cancer

    International Nuclear Information System (INIS)

    Thirty patients with anti-carcinoembryonic antigen (CEA)-producing cancers of the colon, breast, or thyroid were intected with 1 to 2 mCi of Iodine-131 (131I)-labeled, affinity-purified, goat or baboon anti-CEA antibodies. Images were obtained daily for four days. Computerized background subtraction using technetium 99m (99mTC)-labeled compounds was used. Images obtained with and without background subtraction were correlated with other evidence of disease. Activity levels in plasma, urine, and thyroid gland were monitored. Significant deiodination of antibody occurred within the first 24 hours. The mean plasma half-disappearance-time of baboon antibody was significantly longer than the mean half-disappearance-time of goat antibody. With exogenous blockade, total thyroid uptake was less than 0.1% of the injected dose. Without background subtraction, scintigraphic localization of known tumor was possible in one of two patients with colon carcinoma, in three of 20 patients with breast cancer, and in one of five patients with medullary carcinoma of the thyroid. With background subtraction, potential false-positive results could be generated for every patients, depending on the normalization site chosen and the degree of subtraction used. In contrast to results of previous reports, CEA-producing tumor was found to be infrequently localized using highly purified goat or primate radiolabeled anti-CEA. Furthermore, the subtraction technique described by previous investigators may lead to a high false-positive rate

  12. Computed tomography of pulmonary changes in rheumatoid arthritis: carcinoembryonic antigen (CEA) as a marker of airway disease.

    Science.gov (United States)

    Koch, Milene Caroline; Pereira, Ivânio Alves; Nobre, Luiz Felipe Souza; Neves, Fabricio Souza

    2016-04-01

    Rheumatoid arthritis (RA) classically affects the joints, but can present extra-articular manifestations, including pulmonary disease. The present study aimed to identify possible risk factors or laboratory markers for lung involvement in RA, particularly the presence of rheumatoid factor (RF), anti-citrullinated peptide antibodies (ACPA), and tumor markers, by correlating them with changes observed on chest high-resolution computerized tomography (HRCT). This cross-sectional study involved RA patients who were examined and questioned by a specialist physician and later subjected to chest HRCT and blood collection for measurement of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), ACPA (anti-vimentin and/or anti-CCP3), and the tumor markers carcinoembryonic antigen (CEA), CA 125, CA 15-3, and CA 19-9. A total of 96 patients underwent chest HRCT. The most frequent findings were bronchial thickening (27/28.1 %) and bronchiectasis (25/26 %). RF was present in 63.2 % of patients (55/87), and ACPA (anti-vimentin or anti-CCP3) was present in 72.7 % of patients (64/88). CEA levels were high in 14 non-smokers (37.8 %) and 23 smokers (62.2 %). CA-19-9 levels were high in 6 of 86 patients (7.0 %), CA 15-3 levels were high in 3 of 85 patients (3.5 %), and CA 125 levels were high in 4 of 75 patients (5.3 %). Multivariate analysis indicated a statistically significant association between high CEA levels and the presence of airway changes in patients with RA (p = 0.048). CEA can serve as a predictor of lung disease in RA and can help identify individuals who require more detailed examination for the presence of respiratory disorders.

  13. Hookah smoking and cancer: carcinoembryonic antigen (CEA levels in exclusive/ever hookah smokers

    Directory of Open Access Journals (Sweden)

    Chaouachi Kamal

    2008-05-01

    Full Text Available Abstract Background We have recently published some work on CEA levels in hookah (also called narghile, shisha elsewhere and cigarette smokers. Hookah smokers had higher levels of CEA than non-smokers although mean levels were low compared to cigarette smokers. However some of them were also users of other tobacco products (cigarettes, bidis, etc.. Objectives To find serum CEA levels in ever/exclusive hookah smokers, i.e. those who smoked only hookah (no cigarettes, bidis, etc., prepared between 1 and 4 times a day with a quantity of up to 120 g of a tobacco-molasses mixture each (i.e. the tobacco weight equivalent of up to 60 cigarettes of 1 g each and consumed in 1 to 8 sessions. Methods Enhanced chemiluminescent immunometric technique was applied to measure CEA levels in serum samples from 59 exclusive male smokers with age ranging from 20–80 years (mean = 58.8 ± 14.7 years and 8–65 years of smoking (mean = 37.7 ± 16.8. 36 non-smokers served as controls. Subjects were divided into 3 groups according to the number of preparations; the number of sessions and the total daily smoking time: Light (1; 1; ≤ 20 minutes; Medium (1–3; 1–3; >20 min to ≤ 2 hrs and Heavy smokers (2–4; 3–8; >2 hrs to ≤ 6 hrs. Because of the nature of distribution of CEA levels among our individuals, Wilcoxon's rank sum two-sample test was applied to compare the variables. Results The overall CEA levels in exclusive hookah smokers (mean: 3.58 ± 2.61 ng/ml; n = 59 were not significantly different (p ≤ 0.0937 from the levels in non-smokers (2.35 ± 0.71 ng/ml. Mean levels in light, medium and heavy smokers were: 1.06 ± 0.492 ng/ml (n = 5; 2.52 ± 1.15 ng/ml (n = 28 and 5.11 ± 3.08 ng/ml (n = 26 respectively. The levels in medium smokers and non-smokers were also not significantly different (p ≤ 0.9138. In heavy smokers, the CEA levels were significantly higher than in non-smokers (p ≤ 0.0001567. Conclusion Overall CEA levels in exclusive hookah

  14. Expression of a neuroendocrine gene signature in gastric tumor cells from CEA 424-SV40 large T antigen-transgenic mice depends on SV40 large T antigen.

    Directory of Open Access Journals (Sweden)

    Fritz Ihler

    Full Text Available BACKGROUND: A large fraction of murine tumors induced by transgenic expression of SV40 large T antigen (SV40 TAg exhibits a neuroendocrine phenotype. It is unclear whether SV40 TAg induces the neuroendocrine phenotype by preferential transformation of progenitor cells committed to the neuroendocrine lineage or by transcriptional activation of neuroendocrine genes. METHODOLOGY/PRINCIPAL FINDINGS: To address this question we analyzed CEA424-SV40 TAg-transgenic mice that develop spontaneous tumors in the antral stomach region. Immunohistology revealed expression of the neuroendocrine marker chromogranin A in tumor cells. By ELISA an 18-fold higher level of serotonin could be detected in the blood of tumor-bearing mice in comparison to nontransgenic littermates. Transcriptome analyses of antral tumors combined with gene set enrichment analysis showed significant enrichment of genes considered relevant for human neuroendocrine tumor biology. This neuroendocrine gene signature was also expressed in 424GC, a cell line derived from a CEA424-SV40 TAg tumor, indicating that the tumor cells exhibit a similar neuroendocrine phenotype also in vitro. Treatment of 424GC cells with SV40 TAg-specific siRNA downregulated expression of the neuroendocrine gene signature. CONCLUSIONS/SIGNIFICANCE: SV40 TAg thus appears to directly induce a neuroendocrine gene signature in gastric carcinomas of CEA424-SV40 TAg-transgenic mice. This might explain the high incidence of neuroendocrine tumors in other murine SV40 TAg tumor models. Since the oncogenic effect of SV40 TAg is caused by inactivation of the tumor suppressor proteins p53 and RB1 and loss of function of these proteins is commonly observed in human neuroendocrine tumors, a similar mechanism might cause neuroendocrine phenotypes in human tumors.

  15. Anti-CEA monoclonal antibody: technetium-99m labeling and the validation process of a scintigraphic animal model with a non-cellular antigenic implant.

    Science.gov (United States)

    Sapienza, Marcelo Tatit; Marques, Fabio Luiz Navarro; Okamoto, Miriam Roseli Yoshie; Hironaka, Fausto Haruki; Buchpiguel, Carlos Alberto

    2002-07-01

    Animal models are currently used to verify the biodistribution of different radiopharmaceuticals before its clinical application in Nuclear Medicine; however, there may be some limitations. The utilization of labelled anti-tumor monoclonal antibodies (MoAb) in experimental models often requires implant of human antigens (usually a cellular implant), which cannot be achieved in immunocompetent animals. Our purpose was to label an anti-CEA MoAb with technetium-99m (99Tc) and to validate a simplified animal model using a noncellular antigenic implant. MoAb was directly labelled with 99mTc, after reduction with 2-mercaptoethanol. Labeling efficiency was checked by ascending chromatography and immunoreactive fraction was measured in plastic wells sensitized with the antigen. Radiopharmaceutical biodistribution was evaluated by dissection and scintigraphy in 5 mice groups; following the subcutaneous administration of Al(OH)3, CEA adsorbed Al(OH)2 and a control group evaluation. Labeling efficiency was 94+/-3%, which showed to be stable for 24 hr, with immunoreactive fraction above 50%. Invasive biodistribution evaluation showed prolonged blood retention, hepatic and renal uptake. A significant increase in uptake was observed in scintigraphic studies of animals with CEA-adsorbed Al(OH)3 implants compared with the other groups (p<0.05). The non-cellular antigenic implant model simplifies the pre-clinical evaluation of labelled MoAb. PMID:12146705

  16. Fully human IgG and IgM antibodies directed against the carcinoembryonic antigen (CEA Gold 4 epitope and designed for radioimmunotherapy (RIT of colorectal cancers

    Directory of Open Access Journals (Sweden)

    Pugnière Martine

    2004-10-01

    Full Text Available Abstract Background Human monoclonal antibodies (MAbs are needed for colon cancer radioimmunotherapy (RIT to allow for repeated injections. Carcinoembryonic antigen (CEA being the reference antigen for immunotargeting of these tumors, we developed human anti-CEA MAbs. Methods XenoMouse®-G2 animals were immunized with CEA. Among all the antibodies produced, two of them, VG-IgG2κ and VG-IgM, were selected for characterization in vitro in comparison with the human-mouse chimeric anti-CEA MAb X4 using flow cytometry, surface plasmon resonance, and binding to radiolabeled soluble CEA and in vivo in human colon carcinoma LS174T bearing nude mice. Results Flow cytometry analysis demonstrated binding of MAbs on CEA-expressing cells without any binding on NCA-expressing human granulocytes. In a competitive binding assay using five reference MAbs, directed against the five Gold CEA epitopes, VG-IgG2κ and VG-IgM were shown to be directed against the Gold 4 epitope. The affinities of purified VG-IgG2κ and VG-IgM were determined to be 0.19 ± 0.06 × 108 M-1 and 1.30 ± 0.06 × 108 M-1, respectively, as compared with 0.61 ± 0.05 × 108 M-1 for the reference MAb X4. In a soluble phase assay, the binding capacities of VG-IgG2κ and VG-IgM to soluble CEA were clearly lower than that of the control chimeric MAb X4. A human MAb concentration of about 10-7 M was needed to precipitate approximatively 1 ng 125I-rhCEA as compared with 10-9 M for MAb X4, suggesting a preferential binding of the human MAbs to solid phase CEA. In vivo, 24 h post-injection, 125I-VG-IgG2κ demonstrated a high tumor uptake (25.4 ± 7.3%ID/g, close to that of 131I-X4 (21.7 ± 7.2%ID/g. At 72 h post-injection, 125I-VG-IgG2κ was still concentrated in the tumor (28.4 ± 11.0%ID/g whereas the tumor concentration of 131I-X4 was significantly reduced (12.5 ± 4.8%ID/g. At no time after injection was there any accumulation of the radiolabeled MAbs in normal tissues. A pertinent analysis of

  17. CEA (Carcinoembryonic Antigen) Test

    Science.gov (United States)

    ... may also be used as a marker for medullary thyroid cancer and cancers of the rectum, lung , ... ulcerative colitis , rectal polyps , emphysema , and benign breast disease. ^ Back to top Proudly sponsored by ... Learn more ...

  18. A comparative study of mucin-like carcinoma-associated antigen (MCA), CA 125, CA 19-9 and CEA in patients with ovarian cancer.

    Science.gov (United States)

    Koelbl, H; Schieder, K; Neunteufel, W; Bieglmayer, C

    1989-01-01

    A mucin-like carcinoma associated antigen (MCA), which is recognized by the monoclonal antibody b-12, was found to be elevated in sera of breast cancer patients. Since an immunohistochemical reaction of the monoclonal antibody b-12 was found in epithelial tumors of the ovary we investigated MCA serum levels in 50 patients with ovarian cancer (mean age 59 years, range 31-81 years). In addition, CA 125, CA 19-9 and CEA were determined to compare sensitivity, specificity and the predictive value of the positive test of each parameter used in this study. Blood samples were obtained in 20 patients with progressive disease and in 30 patients during disease free intervals. The MCA serum levels of patients with progressive ovarian cancer (mean +/- SD: 14.7 +/- 14.6 U/ml) did not differ significantly from those of patients in remission (mean +/- SD: 8.2 +/- 5.3 U/ml) or from values of a healthy control group (mean +/- SD: 7.7 +/- 3.8 U/ml, n = 70). Women with progressive disease displayed significantly higher CA 125 (p less than 0.0001) and CEA (p less than 0.0063) serum levels than patients in remission. No significant difference was found for CA 19-9 in patients with ovarian cancer, irrespective of the clinical status. Considering marker surge and tumor progression, the highest sensitivity was found for CA 125 (75%). Sensitivities of the other markers were significantly lower and reached only 25-35%. The predictive value of elevated marker levels as well as specificity of the marker substances were similar. Sensitivity could be extended to 90% if elevation of CA 125, CA 19-9, CEA and MCA were taken into consideration, however specificity was lowered by using this marker combination.

  19. Polyethylene glycol (PEG) linked to near infrared (NIR) dyes conjugated to chimeric anti-carcinoembryonic antigen (CEA) antibody enhances imaging of liver metastases in a nude-mouse model of human colon cancer.

    Science.gov (United States)

    Maawy, Ali A; Hiroshima, Yukihiko; Zhang, Yong; Luiken, George A; Hoffman, Robert M; Bouvet, Michael

    2014-01-01

    We report here that polyethylene glycol (PEG) linked to near infrared dyes conjugated to chimeric mouse-human anti-carcinoembryonic antigen (CEA) antibody greatly improves imaging of liver metastases in a nude mouse model of colon-cancer experimental metastases. PEGylated and non-PEGylated DyLight 650 and 750 dyes were conjugated to the chimeric anti-CEA antibody. The dyes were initially injected intravenously into nude mice without tumors. Tissue biodistribution was determined by tissue sonication and analyzing tissue dye concentration profiles over time. PEGylated dyes had significantly lower accumulation in the liver (p = 0.03 for the 650 dyes; p = 0.002 for the 750 dyes) compared to non-PEGylated dyes. In an experimental liver metastasis model of HT-29 colon cancer, PEGylated dyes conjugated to the anti-CEA antibody showed good labeling of metastatic tumors with high contrast between normal and malignant tissue which was not possible with the non-PEGylated dyes since there was so much non-specific accumulation in the liver. PEGylation of the DyLight 650 and 750 NIR dyes significantly altered tissue biodistribution, allowing brighter tissue labeling, decreased accumulation in normal organs, particularly the liver. This enabled high fidelity and high contrast imaging of liver metastases.

  20. Polyethylene glycol (PEG linked to near infrared (NIR dyes conjugated to chimeric anti-carcinoembryonic antigen (CEA antibody enhances imaging of liver metastases in a nude-mouse model of human colon cancer.

    Directory of Open Access Journals (Sweden)

    Ali A Maawy

    Full Text Available We report here that polyethylene glycol (PEG linked to near infrared dyes conjugated to chimeric mouse-human anti-carcinoembryonic antigen (CEA antibody greatly improves imaging of liver metastases in a nude mouse model of colon-cancer experimental metastases. PEGylated and non-PEGylated DyLight 650 and 750 dyes were conjugated to the chimeric anti-CEA antibody. The dyes were initially injected intravenously into nude mice without tumors. Tissue biodistribution was determined by tissue sonication and analyzing tissue dye concentration profiles over time. PEGylated dyes had significantly lower accumulation in the liver (p = 0.03 for the 650 dyes; p = 0.002 for the 750 dyes compared to non-PEGylated dyes. In an experimental liver metastasis model of HT-29 colon cancer, PEGylated dyes conjugated to the anti-CEA antibody showed good labeling of metastatic tumors with high contrast between normal and malignant tissue which was not possible with the non-PEGylated dyes since there was so much non-specific accumulation in the liver. PEGylation of the DyLight 650 and 750 NIR dyes significantly altered tissue biodistribution, allowing brighter tissue labeling, decreased accumulation in normal organs, particularly the liver. This enabled high fidelity and high contrast imaging of liver metastases.

  1. Clinical evaluation of radioassays for calcitonin, CEA-S, AFP and ferritin as tumor markers

    International Nuclear Information System (INIS)

    Clinical usefulness of the radioassays for serum calcitonin, isometric species of carcinoembryonic antigen (CEA-S), alpha-fetoprotein (AFP) and ferritin as the specific tumor markers was evaluated. The calcitonin assay was found to be extremely useful in detecting the cases of familial medullary carcinoma of the thyroid and in monitoring the patients after surgery. The CEA-S assay was proved more specific in titers and positivity for malignancies, especially GI tract carcinomas than CEA assay. The diagnosis of hepatoma could be performed precisely with a combination of 67Ga scintigraphy and assays of CEA and AFP. The serum ferritin levels in malignancies overlapped widely with those of non-malignancies. However, the estimation of ferritin-iron ratio was thought to be a useful means for screening patients with suspicious lesions. (author)

  2. Half-Antibody Functionalized Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery to Carcinoembryonic Antigen (CEA) Presenting Pancreatic Cancer Cells

    Science.gov (United States)

    Hu, Che-Ming Jack; Kaushal, Sharmeela; Tran Cao, Hop S.; Aryal, Santosh; Sartor, Marta; Esener, Sadik; Bouvet, Michael; Zhang, Liangfang

    2010-01-01

    Current chemotherapy regimens against pancreatic cancer are met with little success as poor tumor vascularization significantly limits the delivery of oncological drugs. High-dose targeted drug delivery, through which a drug delivery vehicle releases a large payload upon tumor localization, is thus a promising alternative strategy against this lethal disease. Herein, we synthesize anti-CEA half-antibody conjugated lipid-polymer hybrid nanoparticles and characterize their ligand conjugation yields, physicochemical properties, and targeting ability against pancreatic cancer cells. Under the same drug loading, the half-antibody targeted nanoparticles show enhanced cancer killing effect compared to the corresponding non-targeted nanoparticles. PMID:20394436

  3. CEA Annual report 2007

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities for the year 2007 in these three main areas: science and technology working for nuclear deterrence and global security, the energies without greenhouse effect gases emission against the climatic change, researches in the information sciences and technologies for a better communication and health. The CEA safety, organization, communication and international relations are also presented. (A.L.B.)

  4. CEA financial report 2007

    International Nuclear Information System (INIS)

    This document provides financial data on the CEA for the year 2007. The management report (budget, resources, expenditures) and the accounting are detailed. The main management events of the year 2007 are presented. (A.L.B.)

  5. Stromal CEA immunoreactivity is correlated with lymphatic invasion of human esophageal carcinoma.

    Science.gov (United States)

    Kijima, H; Oshiba, G; Kenmochi, T; Kise, Y; Tanaka, H; Chino, O; Shimada, H; Ueyama, Y; Tanaka, M; Makuuchi, H

    2000-04-01

    Carcinoembryonic antigen (CEA) is a good marker of colorectal cancer. Recent studies have demonstrated that CEA may function as a metastatic potentiator by different pathways; i.e. modulation of immune responses, facilitation of intercellular adhesion and cellular migration. However, expression patterns of CEA have not yet been established in human esophageal carcinomas. In this study, we examined CEA expression in human esophageal squamous cell carcinoma and its clinicopathological significance. CEA immunoreactivity was frequently detected in the cancer cells (cytoplasmic type; 81.1%, 43/53) as well as in the cancer stroma (stromal type; 32.1%, 17/53), regardless of the depth of tumor invasion. Lymphatic invasion of cancer cells was frequently found in the stromal CEA-positive esophageal cancer (44.4%, 16/36), compared to stromal CEA-negative cancer (5.9%, 1/17) (pCEA expression plays important roles in lymphatic invasion of human esophageal squamous cell carcinoma.

  6. CEA 2005 annual report

    International Nuclear Information System (INIS)

    This document is the 2005 activity report of the French atomic energy commission (CEA). The CEA is a main actor of nuclear research, development and innovation and is involved in three main domains: energy, defense/security, and information/health technologies thanks to high quality research works. With a manpower of 15000 researchers and collaborators with internationally acknowledged competences, the CEA is a driving force of industrial innovation and develops partnerships with French and European industries. It also warrants the perenniality of nuclear dissuasion. This report presents these different aspects of the CEA activities: 1 - defense-security: simulation program, opening to the scientific community, nuclear warheads, nuclear propulsion, cleansing of Rhone valley facilities, permanent monitoring of treaties respect, fight against terrorism; 2 - energy: optimization of the industrial park, advances in long lived radioactive wastes management, future nuclear systems, cleansing and dismantling integration, European nuclear energy research, new energy technologies; 3 - information and health technologies: major challenge of micro- and nano-technologies, key role of software technologies and complex systems; 4 - big research facilities opened to the scientific and industrial communities; 5 - scientific status: scientific evaluation process, prices and honors; 6 - programs support: revisited strategic control, confirmed simplification, active employment and training policy, teaching and training, technological valorization, international relations, communication, continuous quality approach, mastery of facilities safety, security, environmental control, a key-year for information systems. A financial report is attached to the document. (J.S.)

  7. CEA 2009 annual report

    International Nuclear Information System (INIS)

    After an indication of several key figures about the activity of the CEA (Centre d'Etudes Atomiques) and its relationship with the academic as well as the industrial field, in France and worldwide, this 2009 annual report presents its various research programs in the field of defence and of global security: basic research (nuclear weapons and propulsion, struggle against proliferation and terrorism) and applied research (nuclear deterrence, national and international security). Then, it presents the programs in the field of de-carbonated energy: basic research (in material science and in life sciences) and applied research (fission energy, fusion energy, new energy technologies). A last group of research programs deals with information and health technologies and concerns life and material sciences, micro- and nano-technologies, software technologies. Interaction with other research institutions and bodies is also evoked. A brief scientific assessment is proposed. Finally, the different structures building the CEA are presented

  8. CEA - Annual report 2006

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in 3 main areas: energy, health care and information technology and defense and security. This annual report presents the CEA activity for the year 2006 in these three main areas: Science and technology working for nuclear deterrence and global security (the simulation programs, the nuclear warheads, the nuclear propulsion, the decommissioning, the fighting against nuclear proliferation and monitoring international treaties, the global security); health and information technology (micro and nano technologies and systems); energy from nuclear fission and fusion and other technologies that do not emit greenhouse gases (progress for the nuclear industry, sustainable management of radioactive materials and waste, nuclear systems of the future, new energy technologies). (A.L.B.)

  9. CEA and mining industry

    International Nuclear Information System (INIS)

    The French atomic energy commission (CEA) is involved in the mining industry in several ways: - in the front-end of the nuclear industry through its daughter companies and participations in the exploration and exploitation of uranium ores, but also of gold and alloy metals with a 26% participation in Eramet company, the world leader of manganese and nickel. This activity is the main occupation of Cogema daughter company, via the Areva holding; - in the back-end of the nuclear fuel cycle with the delicate problem of the management of radioactive wastes; - in parallel with the nuclear industry through an important activity in semiconductor materials (FCI and SMTElectronics); - and finally through various research works on several mineral compounds. This article focusses on the fuel cycle aspects of the CEA activities and concludes with the research works carried out today on thermonuclear fusion. (J.S.)

  10. CEA blood test

    Science.gov (United States)

    Carcinoembryonic antigen blood test ... A blood sample is needed . ... When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging sensation. Afterward, there may be some throbbing or a slight bruise. ...

  11. CEA Annual report 2007; CEA rapport annuel 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities for the year 2007 in these three main areas: science and technology working for nuclear deterrence and global security, the energies without greenhouse effect gases emission against the climatic change, researches in the information sciences and technologies for a better communication and health. The CEA safety, organization, communication and international relations are also presented. (A.L.B.)

  12. CEA sustainable development report 2007; CEA rapport developpement durable 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities in the domain of the sustainable development. The first part is devoted to the environment preservation policy (energy, water, air, chemistry, wastes, transport, buildings). The second part shows the dynamic governance in the domain of the risks management. The last part presents the CEA activities of research for the sustainable development. (A.L.B.)

  13. Clinical significant of combined determination of serum CEA and carbohydrate antigen tumor markers in 85 patients with lung adenocarcinoma%肺腺癌患者血清CEA和糖链抗原肿瘤标记物联合测定的临床意义

    Institute of Scientific and Technical Information of China (English)

    沈粉秧

    2014-01-01

    Objective To explore the clinical significant of combined determination of serum CEA and car-bohydrate antigen (CA125, CA199, CA153) tumor markers in patients with lung adenocarcinoma. Methods The serum CEA and carbohydrate antigen (CA125, CA199 and CA153) levels were determined with chemilumescence immunoassay in 85 patients with lung adenocarcinoma, 35 patients with lung squanous carcinoma, 42 patients with small cell lung cancer and 49 patients with benign lung disease. Results In 162 patients with lung cancer, the ser-um CEA and carbohydrate antigen levels were significantly higher in 85 patients with lung adenocarcinoma, 35 pa-tients with lung squanous carcinoma and 42 patients with small cell lung cancer than those in 49 patients with benign lung disease (P<0. 001) and the level of 85 patients with lung adenocarcinoma was the highest. So did the serum carbohydrate antigen levels. The sensitivity (95. 29%) and accuracy (83. 76%) in the four tumor markers combined determination were significantly higher than those in single item ( P all<0. 01 ) , and the specificity was 75. 23%. Conclusion The combined detection of serum CEA and carbohydrate antigen levels is helpful for the diagnosis of lung adenocarcinoma, with higher sensitivity and accuracy.%目的:为了探讨85例肺腺癌患者血清CEA和糖链抗原肿瘤标记物水平联合测定的临床意义。方法化学发光免疫分析测定85例肺腺癌、35例肺鳞癌、42例小细胞肺癌和49例良性肺部疾病血清CEA和糖链抗原(CA125、CA199和CA153)水平,并进行了比较性研究。结果162例肺癌患者中,85例肺腺癌、35例肺鳞癌、42例小细胞肺癌患者血清CEA水平较之49例良性肺部疾病明显增高( P<0.001、P<0.01和P<0.001),以肺腺癌增高最为明显。血清糖链抗原(CA125、CA199和 CA153)水平亦然,也以肺腺癌血清CA125、CA199和CA153水平增高最为显著。四项肿瘤标记物的联合测定对85例肺腺癌的敏感性为95.29

  14. CEA financial report 2007; CEA rapport financier 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This document provides financial data on the CEA for the year 2007. The management report (budget, resources, expenditures) and the accounting are detailed. The main management events of the year 2007 are presented. (A.L.B.)

  15. CEA - 2014 risk management assessment

    International Nuclear Information System (INIS)

    After introducing presentations of CEA managers in charge of risk management and controls, this document presents and comments the actions undertaken by the CEA and the obtained results in terms of risk management in different fields: protection and control of the environment, installation safety, health, safety and radiation protection, transport of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of legal risks, internal audits and controls. Other topics are addressed like the presentation of the risk management department, and the role of the CEA in the relationship between research and industry

  16. CEA sustainable development report 2007

    International Nuclear Information System (INIS)

    The CEA, a prominent player in research development and innovation, is active in three main domains: energy, health care and information technology, defense and security. This annual report presents the CEA activities in the domain of the sustainable development. The first part is devoted to the environment preservation policy (energy, water, air, chemistry, wastes, transport, buildings). The second part shows the dynamic governance in the domain of the risks management. The last part presents the CEA activities of research for the sustainable development. (A.L.B.)

  17. Scientific evaluation at the CEA; Evaluation scientifique au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-11-01

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1998. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 5 parts dealing successively with: part 1 - the CEA, a public research organization (civil nuclear research, technology research and transfers, defence activities); the scientific and technical evaluation at the CEA (general framework, evaluation of the IPSN and DAM); part 2 - the scientific and technical councils (directions of fuel cycle, of nuclear reactors, and of advanced technologies); part 3 - the scientific councils (directions of matter and of life sciences); the nuclear protection and safety institute; the direction of military applications; part 4 - the corresponding members of the evaluation; part 5 - the list of scientific and technical councils and members. (J.S.)

  18. CEA Annual progress report 1986

    International Nuclear Information System (INIS)

    This annual report presents the general organization of the CEA, the international relations and politics in nuclear field, the activities (military application, nuclear applied research, ANDRA (National Agency for Radioactive Waste Management), nuclear safety and protection, fundamental research, applied research other than nuclear), the industrial group; among topics about men and means, the budget execution of the public establishment of research. In annex, the nuclear power plants around the world and the principal legislative texts related to CEA or atomic energy published in 1986

  19. CEA 2005 annual report; CEA rapport annuel 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This document is the 2005 activity report of the French atomic energy commission (CEA). The CEA is a main actor of nuclear research, development and innovation and is involved in three main domains: energy, defense/security, and information/health technologies thanks to high quality research works. With a manpower of 15000 researchers and collaborators with internationally acknowledged competences, the CEA is a driving force of industrial innovation and develops partnerships with French and European industries. It also warrants the perenniality of nuclear dissuasion. This report presents these different aspects of the CEA activities: 1 - defense-security: simulation program, opening to the scientific community, nuclear warheads, nuclear propulsion, cleansing of Rhone valley facilities, permanent monitoring of treaties respect, fight against terrorism; 2 - energy: optimization of the industrial park, advances in long lived radioactive wastes management, future nuclear systems, cleansing and dismantling integration, European nuclear energy research, new energy technologies; 3 - information and health technologies: major challenge of micro- and nano-technologies, key role of software technologies and complex systems; 4 - big research facilities opened to the scientific and industrial communities; 5 - scientific status: scientific evaluation process, prices and honors; 6 - programs support: revisited strategic control, confirmed simplification, active employment and training policy, teaching and training, technological valorization, international relations, communication, continuous quality approach, mastery of facilities safety, security, environmental control, a key-year for information systems. A financial report is attached to the document. (J.S.)

  20. Combined detection of preoperative serum CEA, CA19-9 and CA242 improve prognostic prediction of surgically treated colorectal cancer patients

    OpenAIRE

    Wang, Jingtao; Xiao WANG; Yu, Fudong; Chen, Jian; Zhao, Senlin; Zhang, Dongyuan; YU, Yang; Liu, Xisheng; Tang, Huamei; Peng, Zhihai

    2015-01-01

    We assessed the prognostic significance of preoperative serum carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9) and carbohydrate antigen 242 (CA242) levels in surgically treated colorectal cancer patients. The relationship of preoperative serum CEA, CA19-9 and CA242 levels with disease characteristics was investigated in 310 patients. Correlation between tumor markers was investigated using Pearson correlation test. Univariate and multivariate survival analyses were used to s...

  1. Construction of CEA siRNA expression vector and its inhibitory effects on the expression of CEA in EC9706 cells%CEA siRNA载体的构建和转染人食管癌EC9706细胞的沉默作用

    Institute of Scientific and Technical Information of China (English)

    Guoqiang Zhao; Sajia Sun; Hong Zheng; Long Feng; Hongyan Yang; Ziming Dong

    2008-01-01

    Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleotide sequences were designed and synthesized according to the encoding sequence of mRNA of CEA. The annealed oligonucleotide fragments were cloned into pRNAT-U6.2 expression vector and identified by sequencing. The recombinant plasmid pRNAT-U6.2-CEA was transfected into EC9706 cells. The expression of CEA in the stable transfected cells was assayed by real time PCR and Western blot. Results: DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pRNAT-U6.2 vector, and CEA expression in the transfected cells was down-regulated significantly by pRNAT-U6.2-CEA at both the mRNA and protein levels. Conclusion: The siRNA expression vector of CEA is successfully constructed and inhibits CEA expression in EC9706 cells. This facilitates further studies of the function of CEA at the molecular level.

  2. INIS, CEA and nuclear terminology; INIS, CEA et terminologie nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Surmont, J.; Brulet, C.; Constant, A.; Guille, N.; Le Blanc, A.; Mouffron, O.; Anguise, P.; Jouve, J.J

    2007-07-01

    This poster, prepared for the fifth edition of the meetings of scientific and technical information professionals (RPIST, Nancy (France)), presents, first, the INIS information system, its content and coverage, the French participation to this system and the role of the CEA-Saclay as France's official representative for this system. Then it presents the INIS thesaurus with its different levels as a terminological tool for the indexing of documents and for searching documents inside the database. Finally, the very first electronic version of the multilingual thesaurus is introduced. Several national INIS centres, including the CEA-Saclay, have contributed to the translation of lists of new terms and of forbidden terms (synonyms). (J.S.)

  3. CEA - Annual report 2006; CEA - Rapport annuel 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    The CEA, a prominent player in research development and innovation, is active in 3 main areas: energy, health care and information technology and defense and security. This annual report presents the CEA activity for the year 2006 in these three main areas: Science and technology working for nuclear deterrence and global security (the simulation programs, the nuclear warheads, the nuclear propulsion, the decommissioning, the fighting against nuclear proliferation and monitoring international treaties, the global security); health and information technology (micro and nano technologies and systems); energy from nuclear fission and fusion and other technologies that do not emit greenhouse gases (progress for the nuclear industry, sustainable management of radioactive materials and waste, nuclear systems of the future, new energy technologies). (A.L.B.)

  4. CEA: risk management assessment 2011

    International Nuclear Information System (INIS)

    This report proposes a qualitative and quantitative overview of CEA activities in the field of risk management during 2011. These activities concerned the impact on the environment, the safety of installations, the management of professional risks (safety and health at work), the radiological protection of workers, the transports of hazardous materials, waste management, protection of sites, installations and heritage, the management of emergency situations, the management of law risks, controls and audits

  5. Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA

    DEFF Research Database (Denmark)

    Høgdall, Claus K; Christensen, Ib J; Stephens, Ross W;

    2002-01-01

    activator (uPAR) and carcinoembryonic antigen (CEA). Significantly shorter survival was found for patients with TN levels below a cut-off point of 7.5 mg/l compared to patients with levels above, as illustrated by Kaplan-Meier curves. By Cox analyses, log TN, log soluble uPAR as well as log CEA were found...

  6. Preparation of 99mTc-Anti CEA MAb and biodistribution test in mice

    International Nuclear Information System (INIS)

    99mTc-antiCEA MAb radiopharmaceutical has been commercially used under the brand name of Zevalin which is used to detect colorectal cancer. Although human MAb is preferred but commercial product is still using murine originated MAb. This antibody binds specifically to carcinoembryonic antigen (CEA) which is overexpressed in colorectal cancer cells. AntiCEA MAb was reduced with diluted 2-mercaptoethanol (1:2000) prior to labelling with 99mTc, and MDP was used as transchelating agent. Labeling efficiency was analysed with chromatography using HSA impregnated ITLC-SG as stationary phase and mixture of ammona-ethanol-water (1:2:5) as eluants to determine polar 99mTc impurities, and ITLC-SG eluted with salin to determine 99mTc-colloid. Stability study was carried out on radio labeled antiCEA MAb stored at room temperature within several hours and on reduced antiCEA MAb stored at -40°C for several weeks. Biodistribution of 99mTc-antiCEA MAb in normal mice was observed 1 hour and 4 hours post injection. Labeling efficiency of antiCEA MAb was 98,53% ± 0.21 % and decreasing to less than 90% after 9 weeks. Radiolabeled anti CEA MAb kept at room temperature was stable within 5 hours post injection, and the frozen kits were stable up to 9 weeks. Biodistribution of 99mTc-antiCEA MAb in normal mice at 1 hour and 4 hours post injection showed high uptake in various organs. (author)

  7. CEA - Assessment of risk management 2013

    International Nuclear Information System (INIS)

    After some introducing texts by CEA managers, this report proposes a rather detailed overview and presentation of CEA activities, objectives and obtained results in different fields: protection and control of the environment, installation safety, health, safety and radiation protection, transports of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of legal risks, internal controls and audits, activity of the risk management department, CEA activities from research to industry

  8. Changes in soluble CEA and TIMP-1 levels during adjuvant chemotherapy for stage III colon cancer

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Christensen, Ib J; Sölétormos, György;

    2010-01-01

    Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested to be a valuable marker in colorectal cancer (CRC), but the effects of chemotherapy on TIMP-1 levels are unknown. The present study evaluated the effect of chemotherapy on TIMP-1 levels in comparison with carcinoembryonic antigen...... (CEA) levels in patients with stage III colon cancer....

  9. Release of carcinoembryonic antigen from human colon cancer cells by phosphatidylinositol-specific phospholipase C.

    OpenAIRE

    Sack, T L; Gum, J R; Low, M G; Y. S. Kim

    1988-01-01

    Carcinoembryonic antigen (CEA) is released from colon cancer cells into the circulation where it is monitored clinically as an indicator of the recurrence or progression of cancer. We have studied the mechanism of CEA membrane attachment and release using the human colonic adenocarcinoma cell line LS-174T, specimens of human colon cancers, and serum from colon cancer patients. CEA release by cells in vitro and in vivo is associated with the conversion of CEA from a membrane-bound, hydrophobic...

  10. A rapid radioimmunoassay for determination of tumor antigens with reference to carcinoembryonic antigen

    International Nuclear Information System (INIS)

    A novel experimental procedure for the determination of carcinoembryonic antigen (CEA) by a solid phase microradioimmunoassay has been developed. Goat anti-CEA antibodies were immobilized by coupling to cyanogen bromide activated filter paper discs. The inhibition of binding of radioiodinated CEA to the discs was proportional to the amount of unlabelled CEA present in the test sample. The experimental procedure involved two steps: (i) the test material containing unlabelled CEA was allowed to react with the antibody coated discs at 37 deg C for 2 hrs., and (ii) a standard amount of 125I-CEA was added to the reaction mixture and incubated for 24 hours at 37 deg C or room temperature. The discs were then washed 4 times and the radioactivity of each disc was determined. The sensitivity of the test in its present state of development was 2.5 ng/ml. (author)

  11. Decontamination and dismantling at the CEA

    International Nuclear Information System (INIS)

    This document presents the dismantling policy at the CEA (French Research Center on the atomic energy), the financing of the decontamination and the dismantling, the regulatory framework, the knowledge and the technology developed at the CEA, the radiation protection, the environment monitoring and the installations. (A.L.B.)

  12. Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein

    International Nuclear Information System (INIS)

    CEA is a tumor-associated antigen abundantly expressed on several cancer types, including those naturally refractory to chemotherapy. The selection and characterization of human anti-CEA single-chain antibody fragments (scFv) is a first step toward the construction of new anticancer monoclonal antibodies designed for optimal blood clearance and tumor penetration. The human MA39 scFv, selected for its ability to recognize a CEA epitope expressed on human colon carcinomas, was first isolated from a large semi-synthetic ETH-2 antibody phage library, panned on human purified CEA protein. Subsequently, by in vitro mutagenesis of a gene encoding for the scFv MA39, a new library was established, and new scFv antibodies with improved affinity towards the CEA cognate epitope were selected and characterized. The scFv MA39 antibody was affinity-maturated by in vitro mutagenesis and the new scFv clone, E8, was isolated, typed for CEA family member recognition and its CEACAM1, 3 and 5 shared epitope characterized for expression in a large panel of human normal and tumor tissues and cells. The binding affinity of the scFv E8 is in a range for efficient, in vivo, antigen capture in tumor cells expressing a shared epitope of the CEACAM1, 3 and 5 proteins. This new immunoreagent meets all criteria for a potential anticancer compound: it is human, hence poorly or not at all immunogenic, and it binds selectively and with good affinity to the CEA epitope expressed by metastatic melanoma and colon and lung carcinomas. Furthermore, its small molecular size should provide for efficient tissue penetration, yet give rapid plasma clearance

  13. Memoirs of a Cea veteran

    International Nuclear Information System (INIS)

    A brief account is given of the way in which nuclear energy has developed in France and elsewhere over the last fifty years: options developed by the French Atomic Energy Commission (CEA), naval propulsion, development of pressurised water reactors, MOX and thorium fuels. Afterwards, the prospectives for the 21. century will be discussed. Considering that natural resources are depleting while releases of both greenhouse gases and world population are increasing, an active energy policy will have to be implemented with due consideration for social equity and solidarity. It is in this context that the developed countries will have to give preference, beyond savings, to renewable sources of energy, including of course, nuclear energy. Nuclear energy can continue to develop in the long term, provided fast breeder technology is developed at some point. As far as transport is concerned, hydrogen technology, which is clean and renewable, is promising, provided it is generated by nuclear energy. (author)

  14. Memoirs of a Cea veteran

    Energy Technology Data Exchange (ETDEWEB)

    Puill, A

    2001-07-01

    A brief account is given of the way in which nuclear energy has developed in France and elsewhere over the last fifty years: options developed by the French Atomic Energy Commission (CEA), naval propulsion, development of pressurised water reactors, MOX and thorium fuels. Afterwards, the prospectives for the 21. century will be discussed. Considering that natural resources are depleting while releases of both greenhouse gases and world population are increasing, an active energy policy will have to be implemented with due consideration for social equity and solidarity. It is in this context that the developed countries will have to give preference, beyond savings, to renewable sources of energy, including of course, nuclear energy. Nuclear energy can continue to develop in the long term, provided fast breeder technology is developed at some point. As far as transport is concerned, hydrogen technology, which is clean and renewable, is promising, provided it is generated by nuclear energy. (author)

  15. CEA - 2012 Annual Report, 2012 Financial Statements

    International Nuclear Information System (INIS)

    In its first part, this report proposes an overview of activities within the CEA. They concern the CEA's programs on low on carbon energies and associated fundamental researches, on defence and global security and associated fundamental researches, on information technologies and associated fundamental researches, on health technologies and associated fundamental researches, and on very large research infrastructures and associated fundamental researches. The second part addresses the scientific assessment, activities related to teaching and training, to innovation towards enterprises, and to support to valorization. It also indicates prices awarded to the CEA. The third part addresses CEA management and institutional relationships, human resources, international relationships, activities related to communication and information diffusion, and risk management. The fourth part describes the CEA organization, its governance and its various bodies. The second volume contains the financial statements for 2012

  16. Prognostic Effect of Pretreatment Serum Carcinoembryonic Antigen Level

    OpenAIRE

    Kim, Chang Hyun; Lee, Soo Young; Kim, Hyeong Rok; Kim, Young Jin

    2015-01-01

    Abstract Many studies have reported the prognostic value of pretreatment serum carcinoembryonic antigen (pre-CEA) levels on colorectal cancer outcomes. However, controversy remains concerning the significance of pre-CEA levels in patients with rectal cancer treated with neoadjuvant chemoradiotherapy (CRT). Our aim in this study was to investigate the prognostic role of the pre-CEA level in patients with locally advanced rectal cancer undergoing neoadjuvant CRT followed by total mesorectal exc...

  17. Metastatic prostate cancer with elevated serum levels of CEA and CA19-9

    Directory of Open Access Journals (Sweden)

    Guang-Dar Juang

    2014-03-01

    Full Text Available Prostate-specific antigen (PSA is well known as a specific tumor marker for prostate cancer, but carcinoembryonic antigen (CEA- and carbohydrate antigen 19-9 (CA19-9-elevating adenocarcinomas originating in the prostate gland are rare. We report a case of metastatic adenocarcinoma of the prostate gland with a high serum level of CEA and CA19-9 in a 78-year-old man in whom prostate cancer (T3N1M1 had been diagnosed 2 years ago and who was treated with androgen deprivation therapy. He visited the emergency department because of a loss of appetite and abdominal pain. The serum CEA and CA19-9 levels were increased to 218.9 ng/mL (normal, <5 ng/mL and 212 ng/mL (normal, <27 ng/mL, respectively. The serum PSA level was slightly elevated (4.41 ng/mL. Computed tomography demonstrated multiple liver metastases, para-aortic lymph node enlargement, and lung metastases. A liver biopsy was performed and the specimen showed high-grade adenocarcinoma with focal positive staining for PSA. Despite chemotherapy with docetaxel, the patient died 3 months after treatment. Based on this case and a review of the literature, an aggressive variant of prostatic carcinoma with a high serum level of CEA and CA19-9 and a low PSA level was shown to progress rapidly with a poor prognosis.

  18. CEA - 2011 annual report, 2011 financial statement

    International Nuclear Information System (INIS)

    The first report, available both in French and English, presents the different current programs: low carbon energies and associated fundamental researches, global defence and safety and associated researches, information technologies and associated researches, technologies for health and associated fundamental researches, very large research infrastructures and associated fundamental researches. It then addresses the CEA openness: assessment, teaching and training, research valorisation, awards, and the support to various programs: steering activity by the CEA, human resources, international relationships, communication, risk management, information systems. The last part describes the CEA organisation. The second report presents the different financial and accounting data and tables

  19. CEA contribution to advanced robotics

    International Nuclear Information System (INIS)

    Due to development of nuclear energy in France, CEA has a long experience in nuclear robotics. It started from remote handling in hot cells and extended to inspections in nuclear reactors, intervention with vehicles, decommissioning of experimental plants, maintenance and intervention in reactors and reprocessing plants. Existing equipments, which are briefly described, show present status of performances for these applications. Research activities are going-on to develop this potential. Main aspects of recent advances in nuclear robotics are detailed to show that each application has some relevance with fusion remote handling needs. In this panel of activities, fusion would profit more directly from reprocessing and reactor maintenance studies, Computer Aided Teleoperation and advanced manipulators developments. But all the projects in nuclear and servicing robotics may have spin-offs for fusion and mention to Eureka projects will underline the necessity for European cooperation in this field. Finally, honor will be rendered to Jean Vertut who had from the first moment a real passion for fusion. (author). 3 refs, 4 figs, 2 tabs

  20. Anti-CEA loaded maghemite nanoparticles as a theragnostic device for colorectal cancer

    Directory of Open Access Journals (Sweden)

    Campos da Paz M

    2012-10-01

    Full Text Available Mariana Campos da Paz,1 Maria de Fátima M Almeida Santos,1 Camila MB Santos,2 Sebastião W da Silva,2 Lincoln Bernardo de Souza,3 Emília CD Lima,3 Renata C Silva,1 Carolina M Lucci,1 Paulo César Morais,2 Ricardo B Azevedo,1 Zulmira GM Lacava11Instituto de Ciências Biológicas; 2Instituto de Física, Universidade de Brasília, Brasília, DF, Brazil; 3Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, BrazilAbstract: Nanosized maghemite particles were synthesized, precoated (with dimercaptosuccinic acid and surface-functionalized with anticarcinoembryonic antigen (anti-CEA and successfully used to target cell lines expressing the CEA, characteristic of colorectal cancer (CRC cells. The as-developed nanosized material device, consisting of surface decorated maghemite nanoparticles suspended as a biocompatible magnetic fluid (MF sample, labeled MF-anti-CEA, was characterized and tested against two cell lines: a high-CEA expressing cell line (LS174T and a low-CEA expressing cell line (HCT116. Whereas X-ray diffraction was used to assess the average core size of the as-synthesized maghemite particles (average 8.3 nm in diameter, dynamic light scattering and electrophoretic mobility measurements were used to obtain the average hydrodynamic diameter (550 nm and the zeta-potential (−38 mV of the as-prepared and maghemite-based nanosized device, respectively. Additionally, surface-enhanced Raman spectroscopy (SERS was used to track the surface decoration of the nanosized maghemite particles from the very first precoating up to the attachment of the anti-CEA moiety. The Raman peak at 1655 cm−1, absent in the free anti-CEA spectrum, is the signature of the anti-CEA binding onto the precoated magnetic nanoparticles. Whereas MTT assay was used to confirm the low cell toxicity of the MF-anti-CEA device, ELISA and Prussian blue iron staining tests performed with both cell lines (LS174T and HCT116 confirm that the as-prepared MF-anti-CEA

  1. CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models.

    Science.gov (United States)

    Tiernan, James P; Ingram, Nicola; Marston, Gemma; Perry, Sarah L; Rushworth, Jo V; Coletta, P Louise; Millner, Paul A; Jayne, David G; Hughes, Thomas A

    2015-01-01

    Fluorescent imaging of colorectal tumor cells would improve tumor localization and allow intra-operative staging, facilitating stratification of surgical resections thereby improving patient outcomes. We aimed to develop and test fluorescent nanoparticles capable of allowing this in vivo. Dye-doped silica nanoparticles were synthesized. Anti-CEA (carcinoembryonic antigen) or control IgGs were conjugated to nanoparticles using various chemical strategies. Binding of CEA-targeted or control nanoparticles to colorectal cancer cells was quantified in vitro, and in vivo after systemic-delivery to murine xenografts. CEA-targeted, polyamidoamine dendrimer-conjugated, nanoparticles, but not control nanoparticles, allowed strong tumor-specific imaging. We are the first to demonstrate live, specific, in vivo imaging of colorectal cancer cells using antibody-targeted fluorescent nanoparticles. These nanoparticles have potential to allow intra-operative fluorescent visualization of tumor cells.

  2. The CEA-Industrie Group of Companies

    International Nuclear Information System (INIS)

    The 1988 financial and technological status of the CEA-Industry Group of Companies is summarized. The activities, technological innovations, and areas of development perspectives of the CEA-Industry Group of Companies, chiefly concentrated in fields relating to nuclear energy, are described. The principal business sectors of the group involve nuclear fuel cycle, nuclear plants and maintenance, computer applications and life science. Some activities of the group are extended to management, construction and financial fields

  3. CEA - Assessment of risk management for 2012

    International Nuclear Information System (INIS)

    This report proposes an overview of the main events, actions performed by the CEA, and facts for 2012 regarding protection and monitoring of the environment, installation safety, occupational health and safety, radiological protection of workers, transportation of hazardous materials, waste management, protection of sites, installations and heritage, emergency situation management, legal risk management, internal controls and audits. It also presents the organisation and action of the risk management department within the CEA

  4. 1999 scientific evaluation at the CEA

    International Nuclear Information System (INIS)

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1999. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 4 parts dealing successively with: 1)the CEA, a public research organization (strategy, research programs, new organization of the CEA activities, civil nuclear research, technology research and transfer, defence activities, transfer of knowledge) 2)the scientific evaluation at the CEA (evaluations of the civil applications of the CEA, IPSN, DAM, INSTN (national institute for nuclear sciences and techniques) 3)synthesis of the 1999 scientific and technical evaluation for each operational directions of the CEA (directions of fuel cycle, of nuclear reactors, of advanced technologies, of materials sciences, of life sciences, of military applications, of the nuclear protection and safety institute and of the national institute for nuclear sciences and techniques) 4)the corresponding members of the evaluation and the list of scientific and technical councils and members

  5. Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients

    Science.gov (United States)

    Tsouma, Aikaterini; Aggeli, Chrysanthi; Lembessis, Panagiotis; Zografos, George N; Korkolis, Dimitris P; Pectasides, Dimitrios; Skondra, Maria; Pissimissis, Nikolaos; Tzonou, Anastasia; Koutsilieris, Michael

    2010-01-01

    AIM: To develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) method detecting circulating tumor cells in the peripheral blood of colorectal cancer (CRC) patients. METHODS: Peripheral blood samples were collected from 88 CRC patients and 40 healthy individuals from the blood donors’ clinic and subsequently analyzed by multiplex RT-RCR for the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) mRNA. The analysis involved determining the detection rates of CEA, CK20 and EGFR transcripts vs disease stage and overall survival. Median follow-up period was 19 mo (range 8-28 mo). RESULTS: Rates of CEA, CK20 and EGFR detection in CRC patients were 95.5%, 78.4% and 19.3%, respectively. CEA transcripts were detected in 3 healthy volunteer samples (7.5%), whereas all control samples were tested negative for CK20 and EGFR transcripts. The increasing number of positive detections for CEA, CK20 and EGFR transcripts in each blood sample was positively correlated with Astler-Coller disease stage (P < 0.001) and preoperative serum levels of CEA (P = 0.029) in CRC patients. Data analysis using Kaplan-Meier estimator documented significant differences in the overall survival of the different CRC patient groups as formed according to the increasing number of positivity for CEA, CK20 and EGFR transcripts. CONCLUSION: These data suggest that multiplex RT-PCR assay can provide useful information concerning disease stage and overall survival of CRC patients. PMID:21157973

  6. Label-free fluorimetric detection of CEA using carbon dots derived from tomato juice.

    Science.gov (United States)

    Miao, Hong; Wang, Lan; Zhuo, Yan; Zhou, Zinan; Yang, Xiaoming

    2016-12-15

    A facile-green strategy to synthesize carbon dots (CDs) with a quantum yield (QY) of nearly 13.9% has been built up, while tomato juice served as the carbon source. Interestingly, not only the precursor of CDs and the whole synthesis procedure were environmental-friendly, but this type of CDs also exhibited multiple advantages including high fluorescent QY, excellent photostability, non-toxicity and satisfactory stability. Significantly, a label-free sensitive assay for detecting carcinoembryonic antigen (CEA) in a continuous and recyclable way has been proposed on the basis of adsorption and desorption of aptamers by the surface of CDs through a competitive mechanism. To be specific, the richness of carboxyl groups of the CDs enabled strong adsorption of ssDNA to the surface of CDs through π-π stacking interactions, resulting in the effective fluorescence quenching by forming CDs-aptamer complexes. The stronger binding affinity between CEA and CEA-aptamer than the π-π stacking interactions has been taken advantage to achieve immediate recovery of the fluorescence of CDs once CEA was introduced. Thereby, quantitative evaluation of CEA concentration in a broad range from 1ngmL(-1) to 0.5ngmL(-1) with the detection limit of 0.3ngmL(-1) was realized in this way. This strategy can be applied in a recyclable way, broadening the sensing application of CDs with biocompatibility. Besides, the CDs were used for cell imaging, potentiating them towards diverse purposes. PMID:27336615

  7. Radioiodination of monoclonal antibody intact anti-CEA

    International Nuclear Information System (INIS)

    The purpose of this study is to examine a convenient system that can be used to iodinate monoclonal antibodies which is rapid, simple, efficient and reproducible, and which can be accomplished in radiopharmaceutical laboratories. It is important to remember that antibodies are sensitive biochemicals, subject to losses of the activity that is essential to their mode of action, namely the ability to bind specific antigen. The advent of solid phase iodination agents has greatly expanded the range of gentle iodination techniques available for iodinating sensitive biological materials. The agent most widely used is the Iodogen (1,3,4,6 tetrachloro-3a-6a diphenylglycoluril) method. Anti-CEA 4C sub(11) IgG sub(2a,k) (prepared in the Ludwig Institute-Sao Paulo-Brazil ) is used as model to evaluate the Iodogen methodology. The miniature chromatographic system, also rapid, accurate, simple, efficient was elaborated to determine the labelling efficiency incorporation of iodine into immunoglobulin, and the radiochemical purity of sup(131)I-anti-CEA. (author)

  8. Identification of a Carcinoembryonic Antigen Gene Family in the Rat

    OpenAIRE

    Kodelja, Vitam; Lucas, Kurt; Barnert, Sabine; Kleist, Sabine von; Thompson, John A.; Zimmermann, Wolfgang

    1989-01-01

    The existence of a carcinoembryonic antigen (CEA)-like gene family in rat has been demonstrated through isolation and sequencing of the N- terminal domain exons of presumably five discrete genes (rnCGM1-5). This finding will allow for the first time the study of functional and clinical aspects of the tumor marker CEA and related antigens in an animal model. Sequence comparison with the corresponding regions of members of the human CEA gene family revealed a relatively low similarity at the am...

  9. Decontamination and dismantling at the CEA; L'assainissement et le demantelement au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This document presents the dismantling policy at the CEA (French Research Center on the atomic energy), the financing of the decontamination and the dismantling, the regulatory framework, the knowledge and the technology developed at the CEA, the radiation protection, the environment monitoring and the installations. (A.L.B.)

  10. Carcinoembryonic antigen continuous epitopes determined by the spot method.

    Science.gov (United States)

    Solassol, I; Granier, C; Pèlegrin, A

    2001-01-01

    Carcinoembryonic antigen (CEA) is a heavily glycosylated tumor-associated protein with an N-A1-B1-A2-B2-A3-B3 domain structure. Circulating CEA immunoassays are used for monitoring digestive cancer patients, and radiolabeled anti-CEA monoclonal antibodies (MAb) are used for the diagnosis and therapy of CEA-positive tumors. The five major nonoverlapping epitopes (Gold 1-5) have been broadly correlated with the domain organization, but there is no precise localization of the epitopes at the sequence level. In an attempt to identify the peptide sequences corresponding to the five Gold epitopes on the CEA molecule, we prepared a set of 227 overlapping fifteen-mer peptides corresponding to the complete CEA sequence with the SPOT method. Using five high affinity MAbs directed against the five CEA Gold epitopes, we demonstrated that none of these epitopes could be mimicked by a fifteen-mer peptide sequence. However, using rabbit and goat anti-CEA sera, we identified six major continuous antigenic regions. All are included in the Ig-like domains of the CEA: two in the A1 domain (residues 120-134 and 153-164), one each in the A2 (329-337) and A3 domains (508-513), one at the junction between the A3 and B3 domains (553-561) and one in the B3 domain (565-573). A very homologous sequence (common residues VSPRL) was mapped in each of the three A domains. Thus, in terms of occurrence of continuous epitopes, the Ig-like domains A1, A2, A3 and B3 seem to be the most antigenic parts of CEA. These peptide sequences should be good candidates for the future development of site-specific anti-CEA MAbs. PMID:11275797

  11. CEA and its radioactive wastes; Le CEA a l'epreuve de ses dechets

    Energy Technology Data Exchange (ETDEWEB)

    Marano, S

    1999-06-01

    CEA annually produces about 3500 tons of radioactive wastes in its 43 basic nuclear installations. CEA ranks third behind EDF and Cogema. Low-level wastes (A wastes) are sent to ANDRA (national agency for the management of nuclear wastes)whereas medium-level wastes (B wastes) are stored by CEA itself. CEA has checked off its storing places and has set up an installation Cedra to process and store ancient and new nuclear wastes. 3 other installations are planned to operate within 6 years: Agate (Cadarache) will treat liquid effluents, Stella (Saclay) will process liquid wastes that are beta or gamma emitters, and Atena (Marcoule) will treat and store radioactive sodium coming from Phenix reactor and IPSN laboratories. The use of plasma torch for vitrifying wastes is detailed, the management of all the nuclear wastes produced by CEA laboratories and installations is presented. (A.C.)

  12. Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients

    Institute of Scientific and Technical Information of China (English)

    Aikaterini; Tsouma; Chrysanthi; Aggeli; Panagiotis; Lembessis; George; N; Zografos; Dimitris; P; Korkolis; Dimitrios; Pectasides; Maria; Skondra; Nikolaos; Pissimissis; Anastasia; Tzonou; Michael; Koutsilieris

    2010-01-01

    AIM: To develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) method detecting cir-culating tumor cells in the peripheral blood of colorectal cancer (CRC) patients. METHODS: Peripheral blood samples were collected from 88 CRC patients and 40 healthy individuals from the blood donors' clinic and subsequently analyzed by multiplex RT-RCR for the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) mRNA. The analysis involved det...

  13. Comparative study of CEA and CA19-9 in esophageal, gastric and colon cancers individually and in combination (ROC curve analysis)

    Institute of Scientific and Technical Information of China (English)

    Bhawna Bagaria; Sadhna Sood; Rameshwaram Sharma; Soniya Lalwani

    2013-01-01

    Objective:To determine the clinical serum levels of carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), individually and in combination, for the diagnosis of 50 healthy subjects and 150 cases of esophageal, gastric, and colon cancers. Methods:The sensitivities of the two markers were compared individually and in combination, with specificity set at 100%. Receiver operating characteristic (ROC) curves were plotted. Results:Serum CEA levels were significantly higher in cancer patients than in the control group. The sensitivity of CEA was determined:in esophageal cancer, sensitivity=28%, negative predictive value (NPV)=61.72%, and AUC=0.742 (SE=0.05), with a significance level of P Conclusion:CEA exhibited the highest sensitivity for colon cancer, and CA19-9 exhibited the highest sensitivity for gastric cancer. Combined analysis indicated an increase in diagnostic sensitivity in esophageal and gastric cancer compared with that in colon cancer.

  14. Variations in Serum CEA and CYFRA21-1 Levels Before and After Surgery Facilitate Prognosis of Non-small Cell Lung Cancer Patients

    OpenAIRE

    Xinchun DUAN; Cui, Yong; Gong, Min; Tian, Feng; Guan SHI; Wu, Bingqun; Liu, Mingliang; Jiayun GUO; Kong, Yuanyuan

    2015-01-01

    Background and objective Serum carcinoembryonic antigen (CEA) and the soluble fragment of cytokeratin 19 (CYFRA21-1) are important tumor markers (TMs) in the preoperative examination of patients with non-small cell lung cancer (NSCLC). However, the prognostic role of these markers in NSCLC patients remains controversial. The aim of the study was to investigate the clinical significance of serum CEA variances and CYFRA21-1 levels for the prognosis of NSCLC patients following surgery. Methods T...

  15. Development of radiopharmaceuticals based on aptamers: selection and characterization of DNA aptamers for CEA

    International Nuclear Information System (INIS)

    Colorectal cancer is among the top four causes of cancer deaths worldwide. Carcinoembryonic antigen (CEA) is a complex intracellular glycoprotein produced by about 90% of colorectal cancers. CEA has been identified as an attractive target for cancer research because of its pattern of expression in the surface cell and its likely functional role in tumorigenesis. Research on the rapid selection of ligands based on the SELEX (systematic evolution of ligands by exponential enrichment) forms the basis for the development of high affinity and high specificity molecules, which can bind to surface determinants of tumour cells, like CEA. The oligonucleotides ligands generated in this technique are called aptamers. Aptamers can potentially find applications as therapeutic or diagnostic tools for many kind of diseases, like a tumor. Aptamers offer low immunogenicity, good tumour penetration, rapid uptake and fast systemic clearance, which favour their application as effective vehicles for radiotherapy. In addition aptamers can be labeled with different radioactive isotopes. The aim of this work was select aptamers binding to the CEA tumor marker. The aptamers are obtained through by SELEX, in which aptamers are selected from a library of random sequences of synthetic DNA by repetitive binding of the oligonucleotides to target molecule (CEA). Analyses of the secondary structure of the aptamers were determined using the m fold toll. Three aptamers were selected to binding assay with target cells. These aptamers were confirmed to have affinity and specific binding for T84 cell line (target cell), showed by confocal imaging. We are currently studying the potential efficacy of these aptamers as targeted radiopharmaceuticals, for use as imaging agents or therapeutic applications. The development of aptamers specific to CEA open new perspectives for colorectal cancer diagnosis and treatment. Acknowledgments: This investigation was supported by the Centro de Desenvolvimento da

  16. Development of radiopharmaceuticals based on aptamers: selection and characterization of DNA aptamers for CEA

    Energy Technology Data Exchange (ETDEWEB)

    Correa, C.R.; Andrade, A.S.R., E-mail: antero@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Augusto-Pinto, L. [BioAptus, Belo Horizonte, MG (Brazil); Goes, A.M., E-mail: goes@icb.ufmg.br [Departamento de Imunologia e Bioquimica. Instituto de Ciencias Biologicas. Universidade Federal de Minas Gerais. Belo Horizonte, MG (Brazil)

    2011-07-01

    Colorectal cancer is among the top four causes of cancer deaths worldwide. Carcinoembryonic antigen (CEA) is a complex intracellular glycoprotein produced by about 90% of colorectal cancers. CEA has been identified as an attractive target for cancer research because of its pattern of expression in the surface cell and its likely functional role in tumorigenesis. Research on the rapid selection of ligands based on the SELEX (systematic evolution of ligands by exponential enrichment) forms the basis for the development of high affinity and high specificity molecules, which can bind to surface determinants of tumour cells, like CEA. The oligonucleotides ligands generated in this technique are called aptamers. Aptamers can potentially find applications as therapeutic or diagnostic tools for many kind of diseases, like a tumor. Aptamers offer low immunogenicity, good tumour penetration, rapid uptake and fast systemic clearance, which favour their application as effective vehicles for radiotherapy. In addition aptamers can be labeled with different radioactive isotopes. The aim of this work was select aptamers binding to the CEA tumor marker. The aptamers are obtained through by SELEX, in which aptamers are selected from a library of random sequences of synthetic DNA by repetitive binding of the oligonucleotides to target molecule (CEA). Analyses of the secondary structure of the aptamers were determined using the m fold toll. Three aptamers were selected to binding assay with target cells. These aptamers were confirmed to have affinity and specific binding for T84 cell line (target cell), showed by confocal imaging. We are currently studying the potential efficacy of these aptamers as targeted radiopharmaceuticals, for use as imaging agents or therapeutic applications. The development of aptamers specific to CEA open new perspectives for colorectal cancer diagnosis and treatment. Acknowledgments: This investigation was supported by the Centro de Desenvolvimento da

  17. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.

    Science.gov (United States)

    Muenzner, Petra; Kengmo Tchoupa, Arnaud; Klauser, Benedikt; Brunner, Thomas; Putze, Johannes; Dobrindt, Ulrich; Hauck, Christof R

    2016-05-01

    Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA) family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa) proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa. PMID:27171273

  18. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.

    Directory of Open Access Journals (Sweden)

    Petra Muenzner

    2016-05-01

    Full Text Available Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa.

  19. Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa

    Science.gov (United States)

    Muenzner, Petra; Kengmo Tchoupa, Arnaud; Klauser, Benedikt; Brunner, Thomas; Putze, Johannes; Dobrindt, Ulrich; Hauck, Christof R.

    2016-01-01

    Attachment to the host mucosa is a key step in bacterial pathogenesis. On the apical surface of epithelial cells, members of the human carcinoembryonic antigen (CEA) family are abundant glycoproteins involved in cell-cell adhesion and modulation of cell signaling. Interestingly, several gram-negative bacterial pathogens target these receptors by specialized adhesins. The prototype of a CEACAM-binding pathogen, Neisseria gonorrhoeae, utilizes colony opacity associated (Opa) proteins to engage CEA, as well as the CEA-related cell adhesion molecules CEACAM1 and CEACAM6 on human epithelial cells. By heterologous expression of neisserial Opa proteins in non-pathogenic E. coli we find that the Opa protein-CEA interaction is sufficient to alter gene expression, to increase integrin activity and to promote matrix adhesion of infected cervical carcinoma cells and immortalized vaginal epithelial cells in vitro. These CEA-triggered events translate in suppression of exfoliation and improved colonization of the urogenital tract by Opa protein-expressing E. coli in CEA-transgenic compared to wildtype mice. Interestingly, uropathogenic E. coli expressing an unrelated CEACAM-binding protein of the Afa/Dr adhesin family recapitulate the in vitro and in vivo phenotype. In contrast, an isogenic strain lacking the CEACAM-binding adhesin shows reduced colonization and does not suppress epithelial exfoliation. These results demonstrate that engagement of human CEACAMs by distinct bacterial adhesins is sufficient to blunt exfoliation and to promote host infection. Our findings provide novel insight into mucosal colonization by a common UPEC pathotype and help to explain why human CEACAMs are a preferred epithelial target structure for diverse gram-negative bacteria to establish a foothold on the human mucosa. PMID:27171273

  20. A prospective study of serum tumour markers carcinoembryonic antigen, carbohydrate antigens 50 and 242, tissue polypeptide antigen and tissue polypeptide specific antigen in the diagnosis of pancreatic cancer with special reference to multivariate diagnostic score.

    OpenAIRE

    Pasanen, P. A.; Eskelinen, M.; Partanen, K.; Pikkarainen, P; Penttilä, I.; Alhava, E

    1994-01-01

    The aim of this study was to assess by a stepwise multivariate discriminant analysis the value of four current serum tumour markers - carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 50 and CA 242 and tissue polypeptide antigen (TPA) - and a new serum tumour marker, tissue polypeptide specific antigen (TPS), in the diagnosis of pancreatic cancer. The serum values were measured in a prospective series of patients with jaundice, with unjaundiced cholestasis and with a suspicion of chro...

  1. Carcino-Embryonic Antigen

    International Nuclear Information System (INIS)

    Tumour marker analysis has increased our understanding of the presence of tumours in the body. Carcino-embryonic antigen, CEA, is one of the best studied tumour markers and has proved an ideal diagnostic adjuvant. It has helped in quantifying the amount of disease present in a patient and thence to make accurate prognosis on the various diagnosed ailments. At UCH, it is observed that there is an increase in cancer related ailments and therefore the need for early diagnosis is more compelling in our environment to mitigate future cost of managing advanced manifestation

  2. Serum and pancreatic juice carcinoembryonic antigen in pancreatic and biliary disease.

    OpenAIRE

    Carr-Locke, D L

    1980-01-01

    Serum and pancreatic juice carcinoembryonic antigen (CEA) concentrations were studied in a group of 144 patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) with a variety of benign and malignant pancreatic and biliary diseases. Serum CEA was found to be a poor diagnostic and discriminating marker for pancreatic disorders and was raised in obstructive jaundice from various causes correlating with serum alkaline phosphatase. A pancreatic juice CEA concentration of greater ...

  3. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    OpenAIRE

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4 − to Au0 o...

  4. CEA, CYFRA 21-1, NSE, and ProGRP in the diagnosis of lung cancer: a multivariate approach

    OpenAIRE

    Gruber, Christine; Hatz, Rudolf; Reinmiedl, Judith; Nagel, Dorothea; Stieber, Petra

    2008-01-01

    We retrospectively studied the single and combined diagnostic value of carcinoembryonic antigen (CEA), cytokeratin fragment 19 (CYFRA 21-1), neuron specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP), which were routinely analysed in patients with lung tumours of unknown origin at the time of admission to hospital. Inclusion criteria were the determination of CEA (AxSYM/Abbott), CYFRA 21-1 (ElecSys/Roche) and NSE (Kryptor/Brahms). We examined 1747 patients, where 1325 suffered f...

  5. Detection of Carcinoembryonic Antigens Using a Surface Plasmon Resonance Biosensor

    Directory of Open Access Journals (Sweden)

    Shin-Ichiro Nishimura

    2008-07-01

    Full Text Available Carcinoembryonic antigen (CEA is an oncofoetal cell-surface glycoprotein that serves as an important tumor marker for colorectal and some other carcinomas. In this work, a CEA immunoassay using a surface plasmon resonance (SPR biosensor has been developed. SPR could provide label-free, real-time detection with high sensitivity, though its ability to detect CEA in human serum was highly dependent on the analytical conditions employed. We investigated the influences of various analytical conditions including immobilization methods for anti-CEA antibody and composition of sensor surface on the selective and sensitive detection of CEA. The results show that anti-CEA antibody immobilized via Protein A or Protein G caused a large increase in the resonance signal upon injection of human serum due to the interactions with IgGs in serum, while direct covalent immobilization of anti-CEA antibody could substantially reduce it. An optimized protocol based on further kinetic analysis and the use of 2nd and 3rd antibodies for the sandwich assay allowed detecting spiked CEA in human serum as low as 25 ng/mL. Furthermore, a self-assembled monolayer of mixed ethylene-glycol terminated alkanethiols on gold was found to have a comparable ability in detecting CEA as CM5 with thick dextran matrix and C1 with short flat layer on gold.

  6. 1999 scientific evaluation at the CEA; L'evaluation scientifique 1999 au CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    This report presents a statement of the scientific and technical activity of the French atomic energy commission (CEA) for the year 1999. This evaluation is made by external and independent experts and requires some specific dispositions for the nuclear protection and safety institute (IPSN) and for the direction of military applications (DAM). The report is divided into 4 parts dealing successively with: 1)the CEA, a public research organization (strategy, research programs, new organization of the CEA activities, civil nuclear research, technology research and transfer, defence activities, transfer of knowledge) 2)the scientific evaluation at the CEA (evaluations of the civil applications of the CEA, IPSN, DAM, INSTN (national institute for nuclear sciences and techniques) 3)synthesis of the 1999 scientific and technical evaluation for each operational directions of the CEA (directions of fuel cycle, of nuclear reactors, of advanced technologies, of materials sciences, of life sciences, of military applications, of the nuclear protection and safety institute and of the national institute for nuclear sciences and techniques) 4)the corresponding members of the evaluation and the list of scientific and technical councils and members.

  7. The CEA contribution to quality control

    International Nuclear Information System (INIS)

    The Commissariat a l'Energie Atomique has developed original methods and techniques for testing delicate components of the primary circuit of pressure water reactors. These techniques make use of a very wide range of non destructive testing methods: Eddy currents, particularly multiple frequencies, for testing steam generator tubes, gudgeon and other pins focused ultrasonics for testing all the welds of the reactor vessel and its cover plate, mixed welds of steam vessels and generators, low welds of the pressurizer and gudgeon pins from the inside. On site use is effected with specific machines intended either for inspecting the tube bundles of steam generators under the responsibility of INTERCONTROLE Co., or for the complete examination of the reactor vessel by mixed CEA/INTERCONTROLE crews under the responsibility of the CEA. All these operations are subjected to a programme of quality assurance that provides the guaranty of execution complying with the procedures in force

  8. CEA: assessment of risk management 2011

    International Nuclear Information System (INIS)

    This report proposes an overview of CEA activities in the field of risk management in different areas: impact on the environment, installation safety, management of occupational risks (occupational health and safety), radiological protection of workers, transportation of hazardous materials, waste management, protection of sites, installations and heritage, management of emergency situations, management of law risks, controls and audits. It finally presents the risk management department

  9. Structural Integrity Analysis of CEA Change Platform

    International Nuclear Information System (INIS)

    The Control Element Assembly Change Platform (CEA CP) is similar to a gantry crane. The CEA CP for Shin-Kori units 3 and 4 (SKN 3 and 4) consists of a bridge, which spans the reactor cavity pool and a gantry superstructure mounted on the bridge. The structure is approximately 8.8 m wide, 4.9 m long and 10.6 m high. The gantry superstructure supports one ton capacity hoist trolley and the bridge supports the In Core Instrumentation (ICI) retrieval cart which moves along the bridge. This paper presents the dynamic and structural analysis of CEA CP which is greater than that of the previous nuclear power plants to verify the structural integrity under the application of the earthquake spectrum. The analysis have been performed using the three orthogonal SSE response spectrum for SKN 3 and 4 which shows much higher acceleration value than OPR- 1000 Plants. In addition, the analyses are performed by 3-dimensional finite element analysis using ANSYS software

  10. High Plasma TIMP-1 and Serum CEA Levels during Combination Chemotherapy for Metastatic Colorectal Cancer Are Significantly Associated with Poor Outcome

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Byström, Per; Berglund, Ake;

    2010-01-01

    Objective: To evaluate whether combination chemotherapy leads to early changes in plasma TIMP-1 and serum carcinoembryonic antigen (CEA) levels in patients with metastatic colorectal cancer (mCRC), and whether such changes relate to subsequent objective response, time to progression (TTP......) and overall survival. Materials and Methods: Eighty-eight patients with mCRC were included. Blood samples were collected before initiation and after 2, 4 and 6 weeks of treatment with an irinotecan-5-fluorouracil combination. Plasma TIMP-1 and serum CEA levels were determined by validated ELISA platforms....... The first response evaluation was performed after 8 weeks of chemotherapy. Results: Median plasma TIMP-1 and serum CEA levels did not change significantly during 6 weeks of treatment. High plasma TIMP-1 and high serum CEA levels before treatment and at weeks 2, 4 and 6 were related to poor objective...

  11. Correlation between Changes in Serum Level of CEA and CYFRA 21-1 and Objective Response of Chemotherapy

    Directory of Open Access Journals (Sweden)

    Xinlin MU

    2009-09-01

    Full Text Available Background and objective Serum levels of tumor markers are associated with tumor metabolism or apoptosis, changes of which after chemotherapy may reflect tumor response to treatment. The aim of this study was to assess the predictive role of changes in serum levels of carcinoembryonic antigen (CEA and cytokeratin 19 fragment (CYFRA 21-1 during chemotherapy in patients with advanced non-small cell lung cancer. Methods Changes in serum levels of CEA and CYFRA 21-1 were investigated retrospectively after one cycle of chemotherapy in 42 patients with advanced NSCLC. Correlations between the changes and radiological objective response were analyzed. Results After two cycles of chemotherapy, radiological objective response rate was 28.6%. At baseline, gender, age, clinical stage, serum levels of CEA and CYFRA 21-1 were not different between patients with objective response (OR and no response (NR. After one cycle of chemotherapy, compared to baseline level, declines in serum levels of CEA and CYFRA 21-1 were observed in patients with OR, but have no statistical significance. In contrast, reduction of CEA and CYFRA 21-1 over baseline after one cycle of chemotherapy showed statistically significant difference between OR and NR. When reduction percentages of CEA and CYFRA 21-1 were used to predict objective response of chemotherapy, the area under the ROC curve (AUC was 0.875 for CEA and 0.919 for CYFRA 21-1. According to the ROC curve, a 22% reduction of CEA yielded a sensitivity of 58.3% and a specificity of 97%, 51% reduction of CYFRA 21-1 with a sensitivity of 83.3% and a specificity of 93.3%. When above reduction percentages were used as cutoffs for prediction of radiological objective response, combination of the CEA and CYFRA 21-1 yielded a sensitivity of 91.7% and a specificity of 86.7%. Conclusion Reduction percentages of CEA and CYFRA 21-1 during chemotherapy could be used to evaluate chemotherapy efficacy in patients with advanced NSCLC. The

  12. The CEA's waste management strategy; La strategie de gestion des dechets du CEA

    Energy Technology Data Exchange (ETDEWEB)

    Behar, Ch. [CEA Saclay, Dir. de l' Energie Nucleaire, 91 - Gif-sur-Yvette (France); Dall' ava, D. [CEA Saclay, Dir. de l' assainissement et du demantelement nucleaire, 91 - Gif-sur-Yvette (France); Fillion, E. [CEA Fontenay-aux-Roses, Direction de la protection et de la surete nucleaire, 92 (France)

    2011-02-15

    The CEA is tasked with carrying out certain research activities: within the Military Applications Division (DAM), research is focused on the nuclear deterrence and, within the Nuclear Energy Division, on developing the industrial nuclear systems of the future and optimising existing nuclear systems in partnership with EDF and AREVA. These major research and development themes entail a need for nuclear research and support facilities which must be maintained at a high level of performance and safety and, also, constantly upgraded to handle the research activities and programmes for which they are used. The CEA strategy is based on the right packaging of the radioactive liquid or solid waste into a form required for its transport, storage or disposal. The Caraibes software allows an efficient traceability of the waste packages. Most of the radioactive effluent processing stations of CEA are being upgraded

  13. Report transparency and nuclear safety 2007 CEA Grenoble; Rapport transparence et securite nucleaire 2007 CEA Grenoble

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Grenoble for the year 2007. Since 2002 the Passage project aims to realize the decontamination and the dismantling of old nuclear installations of the CEA Grenoble. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the year 2007 saw two main steps of the Passage project: the decommissioning of the Siloette reactor, a public consultation about the Lama laboratory dismantling. (A.L.B.)

  14. Remote manipulators and mobile robots in the CEA; Telemanipulation et robotique mobile au CEA

    Energy Technology Data Exchange (ETDEWEB)

    Detriche, J.M. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. des Procedes et Systemes Avances

    1995-05-01

    Thanks to Jean Vertut`s works in the seventies, the CEA has been a pioneer in nuclear remote manipulation and mobile robots conception and has developed innovating systems in response to industry request. During the nineties, improvements in computer control systems have led to the realization of functional demonstrators. In 1995, advances in technology have allowed to consider the first industrial applications of nuclear remote manipulators and robots. This paper gives a review of the development and research works carried out by the CEA in this domain since the seventies, and also some future prospects. (J.S.). 10 figs.

  15. Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site.

    OpenAIRE

    Hefta, S A; Hefta, L J; Lee, T.D. (Taunia D.); Paxton, R J; Shively, J. E.

    1988-01-01

    The COOH-terminal amino acid of carcinoembryonic antigen (CEA) is shown to covalently link with ethanolamine, evidence consistent with the anchorage of CEA to the plasma membrane through a phosphatidylinositol-glycan tail. Purified CEA was digested with trypsin, and the resulting peptides were isolated by reverse-phase HPLC. Tryptic hexapeptide T12, terminating atypically with alanine, corresponded in sequence (Ser-Ile-Thr-Val-Ser-Ala) with the last six residues (637-642) of the third repeati...

  16. Algunas orquidáceas americanas

    OpenAIRE

    Williams, Louis O.

    2012-01-01

    La presente contribución al conocimiento de las Orquidáceas americanas trata de algunas especies de México, América Central, Sur América y las Antillas. Estas notas son el resultado de determinaciones de varios ejemplares colectados en tales regiones, y que se hallan depositados en el Ames Herbarium, Cambridge, Mass., el United States National Museum, Washington, D. C., el New York Botanical Garden Herbarium, Nueva York, o en el Herbarium of the Missouri Botanical Garden, St.Louis, Missouri....

  17. Diagnostic value of combinative detection of serum carcinoembryonic antigen(CEA),adenosine deaminase(ADA),lactate dehydrogenase(LDH) and ESR in differential diagnosis of pleural effusion%联合检测CEA、ADA、LDH及血沉对胸腔积液性质判断的临床意义

    Institute of Scientific and Technical Information of China (English)

    夏静; 闫雪波; 刘荣玉

    2013-01-01

    Objective To establish the diagnostic value of combinative detection of serum CEA,serum and pleural fluid LDH,pleural fluid ADA and ESR in different pleural effusion patients. Methods From December 2011 to June 2012 ,89 cases of in-patients with pleural effusion in the First Affiliated Hospital of Anhui Medical University were chosen,including 25 cases of tuberculous effusion( TE ),42 patients with malignant pleural effusion( MPE ) and 22 cases of inflammatory patients with pleural effusion( IPE ). The levels of serum CEA were examined by electrochemilumin-escence immunoassay method, serum LDH by the enzymatic( L method ), pleural fluid LDH and ADA by rating method and ESR by Westergren. Results The serum CEA of malignant pleural effusion patients is higher than that of TE and IPE patients;The serum and pleural fluid LDH levels in TE,MPE,IPE patients showed no significant difference,but the three pleural effusion LDH levels were significantly higher than the normal range, and the ratio of pleural effusion and serum LDH in patients with TE was significantly higher than that of patients with MPE; The pleural fluid ADA levels of TE were significantly higher than those of the MPE and IPE; The IPE patients ESR levels were significantly higher than those of TE and MPE patients, and ESR levels of TE patients were significantly higher than those of MPE patients. Conclusion Combined detection of serum CEA, pleural fluid and serum LDH, pleural fluid ADA and ESR has great significance in judging the nature of pleural fluid.%目的 探讨联合检测血清癌胚抗原(CEA)、血清及胸水乳酸脱氢酶(LDH)、胸水腺苷脱氨酶(ADA)及血沉对胸腔积液性质判断的临床意义.方法 以安徽医科大学第一附属医院2011年12月至2012年6月89例住院胸腔积液患者为研究对象,其中25例结核性胸腔积液患者,42例恶性胸腔积液患者,22例炎性胸腔积液患者;采用电化学发光免疫分析法测定血清CEA,酶法(L法)测定血

  18. Changes in soluble CEA and TIMP-1 levels during adjuvant chemotherapy for stage III colon cancer

    DEFF Research Database (Denmark)

    Aldulaymi, Bahir; Christensen, Ib Jarle; Sölétormos, György;

    2010-01-01

    Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested to be a valuable marker in colorectal cancer (CRC), but the effects of chemotherapy on TIMP-1 levels are unknown. The present study evaluated the effect of chemotherapy on TIMP-1 levels in comparison with carcinoembryonic antige...... (CEA) levels in patients with stage III colon cancer.......Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been suggested to be a valuable marker in colorectal cancer (CRC), but the effects of chemotherapy on TIMP-1 levels are unknown. The present study evaluated the effect of chemotherapy on TIMP-1 levels in comparison with carcinoembryonic antigen...

  19. Charting a course through the CEAs: diagnosis and management of medullary thyroid cancer.

    Science.gov (United States)

    Rowe, Christopher W; Bendinelli, Cino; McGrath, Shaun

    2016-09-01

    Medullary thyroid cancer (MTC) is an uncommon thyroid cancer that requires a high index of suspicion to facilitate diagnosis of early-stage disease amenable to surgical cure. The challenges of diagnosis, as well as management in the setting of persistent disease, are explored in the context of a case presenting with the incidental finding of elevated carcinoembryonic antigen (CEA) and an (18) F-fluorodeoxyglucose positron emission tomography ((18) F-FDG-PET)-positive thyroid incidentaloma detected following treatment of colorectal cancer. Strategies to individualize prognosis, and emerging PET-based imaging modalities, particularly the potential role of (18) F-DOPA-PET in staging, are reviewed. PMID:27230389

  20. Plasma TIMP-1 and CEA in detection of primary colorectal cancer: a prospective, population based study of 4509 high-risk individuals

    DEFF Research Database (Denmark)

    Nielsen, Hans J; Brünner, Nils; Olsen, Jesper;

    2011-01-01

    Objective. The combination of plasma tissue inhibitor of metalloproteinases-1 (TIMP-1) and carcinoembryonic antigen (CEA) may be valuable biomarkers for early detection of colorectal cancer (CRC). A prospective, population based study was performed to validate this hypothesis. Material and method...

  1. Receiver operating characteristic (ROC) curve analysis of the tumour markers CEA, CA 50 and CA 242 in pancreatic cancer; results from a prospective study.

    OpenAIRE

    Pasanen, P. A.; Eskelinen, M.; Partanen, K.; Pikkarainen, P; Penttilä, I.; Alhava, E

    1993-01-01

    The serum values of the tumour markers carcinoembryonic antigen (CEA), cancer-associated carboanhydrate antigens CA 50 and CA 242 were evaluated in 193 patients with hepatopancreato-biliary diseases by receiver operating characteristic (ROC) curve analysis in order to compare their diagnostic accuracy in pancreatic cancer (n = 26), and to define optimal cut-off levels for the serum values of these tumour markers in the diagnosis of pancreatic cancer. The ROC analysis showed that all marker te...

  2. Peritoneal lavage cytology and carcinoembryonic antigen determination in predicting peritoneal metastasis and prognosis of gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Ji-Kun Li; Miao Zheng; Chuan-Wen Miao; Jian-Hai Zhang; Guang-Han Ding; Wen-Shen Wu

    2005-01-01

    AIM: To evaluate the role of peritoneal lavage cytology (PLC) and carcinoembryonic antigen (CEA) determination of peritoneal washes (pCEA) in predicting the peritoneal metastasis and prognosis after curative resection of gastric cancer.METHODS: PLC and radioimmunoassay of CEA were performed in peritoneal washes from 64 patients with gastric cancer and 8 patients with benign diseases.RESULTS: The positive rate of pCEA (40.6%) was significantly higher than that of PLC (23.4%) (P<0.05).The positive rates of PLC and pCEA correlated with the depth of tumor invasion and lymph node metastasis (P<0.05). pCEA was found to have a higher sensitivity and a lower false-positive rate in predicting peritoneal metastasis after curative resection of gastric cancer as compared to PLC. The 1-, 3-, and 5-year survival rates of patients with positive cytologic findings or positive pCEA results were significantly lower than those of patients with negative cytologic findings or negative pCEA results (P<0.05). Multivariate analysis indicated that pCEA was an independent prognostic factor for the survival of patients with gastric cancer.CONCLUSION: Intraoperative pCEA is a more sensitive and reliable predictor of peritoneal metastasis as well as prognosis in patients with gastric cancer as compared to PLC method.

  3. A carcinoembryonic antigen-secreting adenocarcinoma arising in tailgut cyst: clinical implications of carcinoembryonic antigen.

    Science.gov (United States)

    Cho, Byoung Chul; Kim, Nam Kyu; Lim, Beom Jin; Kang, Sang Ook; Sohn, Ju Hyuk; Roh, Jae Kyung; Choi, Sang Tae; Kim, Sung Ai; Park, Se Eun

    2005-08-31

    Tailgut cysts (TGCs) are rare congenital cysts that occur in the retrorectal or presacral spaces. Although most tailgut cysts have been reported as benign, there have been at least 9 cases associated with malignant change. We report herein on an unusual case of a 40-year-old woman with a carcinoembryonic antigen (CEA)-producing adenocarcinoma arising within a TGC who underwent surgical resection and local radiation therapy. Despite the complete resection, metastatic adenocarcinoma developed five months after surgery. CEA-producing adenocarcinoma from a TGC is extremely rare and only two cases, including this case, have been reported in the English medical literature. Besides CEA, the serum levels of CA 19-9 became markedly elevated in this patient. Given that the serum CEA level decreased to the normal range after complete resection of tumor and that the tumor recurrence was associated with a rebound of the CEA serum level, our case shows that serial measurements of serum CEA can be used for treatment planning and for assessing the patient's treatment response for this rare disease. PMID:16127782

  4. COLONOSCOPY AND CARCINOEMBRYONIC ANTIGEN VARIATIONS

    Directory of Open Access Journals (Sweden)

    Rita G SOUSA

    2014-03-01

    Full Text Available Context Colonoscopy is essential for synchronous and metachronous cancer detection. Carcinoembryonic antigen is a colorectal cancer tumor marker, important as a follow-up tool in patients with previous colorectal cancer. False-positive carcinoembryonic antigen elevation results in multiples exams and in patient anxiety. In literature, there is reference to transient carcinoembryonic antigen increase with colonoscopy. Objective To evaluate the influence of bowel preparation and colonoscopy in carcinoembryonic antigen blood levels. Methods We prospectively studied subjects that underwent routine colonoscopy in our institution. Blood samples were collected (1 before bowel cleaning, (2 before colonoscopy and (3 immediately after colonoscopy. Blood carcinoembryonic antigen levels were determined by “Sandwich” immunoassay. The statistical methods used were the paired t-test and ANOVA. Results Thirty-seven patients (22M/15F were included; age range 28-84 (mean 56 years. Mean carcinoembryonic antigen values were 1.9, 2 and 1.8 for (1, (2 and (3, respectively. An increase in value (2 compared with (1 was observed in 20/37 patients (P = 0.018, mainly in younger patients and in patients requiring more endoluminal interventions. In 29/37 patients, the CEA value decreased from (2 to (3 (P = 1.3x10-7. Conclusions A trend for carcinoembryonic antigen increase after bowel cleaning was observed, especially in younger patients and in patients with more endoluminal interventions, but without clinical meaning.

  5. Radionuclide-Based Cancer Imaging Targeting the Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Hao Hong

    2008-01-01

    Full Text Available Carcinoembryonic antigen (CEA, highly expressed in many cancer types, is an important target for cancer diagnosis and therapy. Radionuclide-based imaging techniques (gamma camera, single photon emission computed tomography [SPECT] and positron emission tomography [PET] have been extensively explored for CEA-targeted cancer imaging both preclinically and clinically. Briefly, these studies can be divided into three major categories: antibody-based, antibody fragment-based and pretargeted imaging. Radiolabeled anti-CEA antibodies, reported the earliest among the three categories, typically gave suboptimal tumor contrast due to the prolonged circulation life time of intact antibodies. Subsequently, a number of engineered anti-CEA antibody fragments (e.g. Fab’, scFv, minibody, diabody and scFv-Fc have been labeled with a variety of radioisotopes for CEA imaging, many of which have entered clinical investigation. CEA-Scan (a 99mTc-labeled anti-CEA Fab’ fragment has already been approved by the United States Food and Drug Administration for cancer imaging. Meanwhile, pretargeting strategies have also been developed for CEA imaging which can give much better tumor contrast than the other two methods, if the system is designed properly. In this review article, we will summarize the current state-of-the-art of radionuclide-based cancer imaging targeting CEA. Generally, isotopes with short half-lives (e.g. 18F and 99mTc are more suitable for labeling small engineered antibody fragments while the isotopes with longer half-lives (e.g. 123I and 111In are needed for antibody labeling to match its relatively long circulation half-life. With further improvement in tumor targeting efficacy and radiolabeling strategies, novel CEA-targeted agents may play an important role in cancer patient management, paving the way to “personalized medicine”.

  6. Simplifying CEA through Excel, VBA, and Subeq

    Science.gov (United States)

    Foster, Ryan

    2004-01-01

    Many people use compound equilibrium programs for very different reasons, varying from refrigerators to light bulbs to rockets. A commonly used equilibrium program is CEA. CEA can take various inputs such as pressure, temperature, and volume along with numerous reactants and run them through equilibrium equations to obtain valuable output information, including products formed and their relative amounts. A little over a year ago, Bonnie McBride created the program subeq with the goal to simplify the calling of CEA. Subeq was also designed to be called by other programs, including Excel, through the use of Visual Basic for Applications (VBA). The largest advantage of using Excel is that it allows the user to input the information in a colorful and user-friendly environment while allowing VBA to run subeq, which is in the form of a FORTRAN DLL (Dynamic Link Library). Calling subeq in this form makes it much faster than if it were converted to VBA. Since subeq requires such large lists of reactant and product names, all of which can't be passed in as an array, subeq had to be changed to accept very long strings of reactants and products. To pass this string and adjust the transfer of input and output parameters, the subeq DLL had to be changed. One program that does this is Compaq Visual FORTRAN, which allows DLLs to be edited, debugged, and compiled. Compaq Visual FORTRAN uses FORTRAN 90/95, which has additional features to that of FORTRAN 77. My goals this summer include finishing up the excel spreadsheet of subeq, which I started last summer, and putting it on the Internet so that others can use it without having to download my spreadsheet. To finish up the spreadsheet I will need to work on debugging current options and problems. I will also work on making it as robust as possible, so that all errors that may arise will be clearly communicated to the user. New features will be added old ones will be changed as I receive comments from people using the spreadsheet

  7. Nomograms for Predicting the Prognostic Value of Pre-Therapeutic CA15-3 and CEA Serum Levels in TNBC Patients.

    Science.gov (United States)

    Dai, Danian; Chen, Bo; Tang, Hailin; Wang, Bin; Zhao, Zhiping; Xie, Xiaoming; Wei, Weidong

    2016-01-01

    Previous studies have indicated that carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3) levels are both independent prognostic factors in breast cancer. However, the utility of CEA and CA15-3 levels as conventional cancer biomarkers in patients with triple-negative breast cancer (TNBC) remains controversial. The current study was performed to explore the predictive value of pre-therapeutic serum CEA and CA15-3 levels, and nomograms were developed including these serum cancer biomarkers to improve the prognostic evaluation of TNBC patients. Pre-therapeutic CA15-3 and CEA concentrations were measured in 247 patients with stage I-IV TNBC. Kaplan-Meier analysis showed that TNBC patients with high levels of both CEA and CA15-3 had shorter overall survival (OS) and disease-free survival (DFS) rates than those in the low-level groups (ppersonal forecasts of OS and DFS for patients with TNBC. These novel nomograms may help physicians to select the optimal treatment plans to ensure the best outcomes for TNBC patients. PMID:27561099

  8. Superconducting quadrupoles for LHC : CERN/CEA-CEN Saclay Collaboration

    CERN Multimedia

    CEA Saclay and CERN Collaboration

    1994-01-01

    Agreement has been signed between CERN and CEA Saclay, concerning the construction of two prototypes of the LHC latest quadrupoles. CEA Saclay was interested with the study, the design, the construction and the testing of this magnet. The fabrication took place at the CEN Laboratory at Saclay in Paris.

  9. Carcinoembryonic antigen promotes colorectal cancer progression by targeting adherens junction complexes

    Energy Technology Data Exchange (ETDEWEB)

    Bajenova, Olga, E-mail: o.bazhenova@spbu.ru [Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation); Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg 199034 (Russian Federation); Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); Chaika, Nina [Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); Tolkunova, Elena; Davydov-Sinitsyn, Alexander [Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064 (Russian Federation); Gapon, Svetlana [Boston Children' s Hospital, Boston, MA 02115 (United States); Thomas, Peter [Department of Surgery and Biomedical Sciences, Creighton University, Omaha, NE 68178 (United States); O’Brien, Stephen [Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg 199034 (Russian Federation)

    2014-06-10

    Oncomarkers play important roles in the detection and management of human malignancies. Carcinoembryonic antigen (CEA, CEACAM5) and epithelial cadherin (E-cadherin) are considered as independent tumor markers in monitoring metastatic colorectal cancer. They are both expressed by cancer cells and can be detected in the blood serum. We investigated the effect of CEA production by MIP101 colorectal carcinoma cell lines on E-cadherin adherens junction (AJ) protein complexes. No direct interaction between E-cadherin and CEA was detected; however, the functional relationships between E-cadherin and its AJ partners: α-, β- and p120 catenins were impaired. We discovered a novel interaction between CEA and beta-catenin protein in the CEA producing cells. It is shown in the current study that CEA overexpression alters the splicing of p120 catenin and triggers the release of soluble E-cadherin. The influence of CEA production by colorectal cancer cells on the function of E-cadherin junction complexes may explain the link between the elevated levels of CEA and the increase in soluble E-cadherin during the progression of colorectal cancer. - Highlights: • Elevated level of CEA increases the release of soluble E-cadherin during the progression of colorectal cancer. • CEA over-expression alters the binding preferences between E-cadherin and its partners: α-, β- and p120 catenins in adherens junction complexes. • CEA produced by colorectal cancer cells interacts with beta-catenin protein. • CEA over-expression triggers the increase in nuclear beta-catenin. • CEA over-expression alters the splicing of p120 catenin protein.

  10. Report transparency and nuclear safety 2007 CEA Saclay; Rapport transparence et securite nucleaire 2007 CEA Saclay

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Saclay for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially two public consultation on release authorizations and the Neurospin installations, the dismantling of the 49 nuclear installation, the shutdown of the learning reactor ULYSSE are detailed. (A.L.B.)

  11. Report transparency and nuclear safety 2007 CEA Cadarache; Rapport transparence et securite nucleaire 2007 CEA Cadarache

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Cadarache for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the report discusses the beginning of the RJH reactor construction, the fourth generation reactors research programs, the implementing of la Rotonde the new radioactive wastes management installation, the renovation of the LECA. (A.L.B.)

  12. Report transparency and nuclear safety 2007 CEA Marcoule; Rapport transparence et securite nucleaire 2007 CEA Marcoule

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Marcoule for the year 2007. Since its creation in 1955 the center realizes industrial and scientific activities relative to the civil and military applications of the radioactivity. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the following two base activities are detailed: Atalante and Phenix. (A.L.B.)

  13. Efficacy of 18F-FDG PET/CT in investigation of elevated CEA without known primary malignancy

    Directory of Open Access Journals (Sweden)

    Simon Sin-man Wong

    2016-01-01

    Full Text Available Aim: To evaluate the efficacy of 18flurodeoxyglucose positron emission tomography/computer tomography (18F-FDG PET/CT in investigating patients with elevated carcinoembryonic antigen (CEA and without known primary malignancy, and the impact of PET/CT findings on patient management. Setting and Design: PET/CT scans done in a tertiary hospital between December 2007 and February 2012 for elevated CEA in patients without known primary malignancy were retrospectively reviewed. Materials and Methods: The PET/CT findings, patients' clinical information, level of CEA, histological diagnosis, and subsequent management were retrieved by the electronic patient record for analysis. Statistical Analysis: Data were analyzed using SPSS version 19. Results: One hundred and one PET/CT scans were performed for patients with elevated CEA. Fifty-eight of these were performed for patients with known primary malignancy and were excluded; 43 PET/CT scans were performed for patients without known primary malignancy and were included. Thirty-three (77% had a positive PET/CT. Among the 32 patients with malignancy, 15 (47% suffered from lung cancer and 8 (25% suffered from colorectal cancer. The sensitivity (97%, specificity (82%, positive predictive value (94%, negative predictive value (90%, and accuracy (93% were calculated. Thirty (91% patients had resultant change in management. The mean CEA level for patients with malignancy (46.1 ng/ml was significantly higher than those without malignancy (3.82 ng/ml (P < 0.05. In predicting the presence of malignancy, a CEA cutoff at 7.55 ng/ml will achieve a sensitivity of 91% and a specificity of 73%. Conclusion: PET/CT, in our study population, appears to be sensitive, specific, and accurate in investigating patients with elevated CEA and without known primary malignancy. In addition to diagnosis of underlying primary malignancy, PET/CT also reveals occult metastases which would affect patient treatment options.Its role in

  14. Transcription Activity of Ectogenic Human Carcinoembryonic Antigen Promoter in Lung Adenocarcinoma Cells A549

    Institute of Scientific and Technical Information of China (English)

    XIONG Weining; FANG Huijuan; XU Yongjian; XIONG Shendao; CAO Yong; SONG Qingfeng; ZENG Daxiong; ZHANG Huilan

    2006-01-01

    The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent protein (GFP) driven by CEA promoter and human cytomegalovirus (CMV) promoter were relatively constructed and named plasmid pCEA-EGFP and pCMV-GFP respectively. The intensity of fluorescence was detected by fluorescence microscope and flow cytometry analysis after the pCEA-GFP and pSNAV-GFP plasmids were transfected into A549 cells through liposome respectively. The results showed (4.08±0.63) % of the A549 cells transfected with pCEA-AFP plasmid expressed, significantly lower than that of the A549 cells transfected with pCMV-GFP [(43.27±3.54) %]. It was suggested that ectogenic human CEA promoter in lung adenocarcinoma cells A549 was weakly expressed. The distinct specificity of CEA promoter in CEA high expression cells was regarded as a tool in selective gene therapy, but the transcription activity of ectogenic human CEA promoter was needed to increase in the future.

  15. Independent prognostic value of preoperative serum markers CA 242, specific tissue polypeptide antigen and human chorionic gonadotrophin beta, but not of carcinoembryonic antigen or tissue polypeptide antigen in colorectal cancer.

    OpenAIRE

    Carpelan-Holmström, M; Haglund, C.; Lundin, J; Alfthan, H.; Stenman, U H; Roberts, P. J.

    1996-01-01

    The prognostic value of preoperative serum concentrations of carcinoembryonic antigen (CEA), CA 242, tissue polypeptide antigen (TPA), specific tissue polypeptide antigen (TPS) and human chorionic gonadotrophin beta (hCG beta) in 251 patients with colorectal cancer (39 Dukes' A, 98 Dukes' B, 56 Dukes' C and 58 Dukes' D) was investigated. When using the cut-off levels recommended for diagnostic purposes, there was a significantly longer overall survival in patients with low tumour marker level...

  16. A combination of preoperative CT findings and postoperative serum CEA levels improves recurrence prediction for stage I lung adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Yamazaki, Motohiko, E-mail: xackey2001@gmail.com [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Ishikawa, Hiroyuki [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Kunii, Ryosuke [Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Tasaki, Akiko; Sato, Suguru; Ikeda, Yohei; Yoshimura, Norihiko [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan); Hashimoto, Takehisa; Tsuchida, Masanori [Division of Thoracic and Cardiovascular Surgery, Niigata University Graduate School of Medical and Dental Sciences (Japan); Aoyama, Hidefumi [Department of Radiology, Niigata University Graduate School of Medical and Dental Sciences (Japan)

    2015-01-15

    Objectives: To assess the prognostic value of combined evaluation of preoperative CT findings and pre/postoperative serum carcinoembryonic antigen (CEA) levels for pathological stage I lung adenocarcinoma. Methods: This retrospective study included 250 consecutive patients who underwent complete resection for ≤3-cm pathological stage I (T1–2aN0M0) adenocarcinomas (132 men, 118 women; mean age, 67.8 years). Radiologists evaluated following CT findings: maximum tumor diameter, percentage of solid component (%solid), air bronchogram, spiculation, adjacency of bullae or interstitial pneumonia (IP) around the tumor, notch, and pleural indent. These CT findings, pre/postoperative CEA levels, age, gender, and Brinkman index were assessed by Cox proportional hazards model to determine the best prognostic model. Prognostic accuracy was examined using the area under the receiver operating characteristic curve (AUC). Results: Median follow-up period was 73.2 months. In multivariate analysis, high %solid, adjacency of bullae or IP around the tumor, and high postoperative CEA levels comprised the best combination for predicting recurrence (P < 0.05). A combination of these three findings had a greater accuracy in predicting 5-year disease-free survival than did %solid alone (AUC = 0.853 versus 0.792; P = 0.023), with a sensitivity of 85.7% and a specificity of 74.3% at the optimal threshold. The best cut-off values of %solid and postoperative CEA levels for predicting high-risk patients were ≥48% and ≥3.7 ng/mL, respectively. Conclusion: Compared to %solid alone, combined evaluation of %solid, adjacency of bullae or IP change around the tumor, and postoperative CEA levels improves recurrence prediction for stage I lung adenocarcinoma.

  17. Serum tumor marker CYFRA 21-1 in the diagnostics of squamous cell lung cancer - comparison with CEA

    International Nuclear Information System (INIS)

    The aim of the study was to test the diagnostic value tumor marker CYFRA 21-1 for squamous cell lung cancer (SQCLC) in comparison with carcinoembryonic antigen (CEA). Ninety-one patients were induced in this study: 56 with SQCLC - Group I, 25 with other types of lung cancer - Group II, 10 with benign respiratory tract diseases - Group III. Median CYFRA 21-1 serum concentration (ng/ml) was: in Group I: 4.52 (0.94 - >16), in Group II: 3.58 (1.72 - >16), in Group III: 2.05 (0.99 - 3.41). Median CEA serum concentration (ng/ml) was: in Group I: 4.49 (076- >20), in Group II: 3.32 (1.17 - >20), in Group III: 3.09 (1.84 - 6.37). There was a highly significant difference between the levels of CYFRA 21-1 in group I and Group III (p < 0.001), but there was no statistically significant difference between the levels of CEA in Group I and III. Sensitivity of CYFRA 21-1 by the cut-off 3.33 ng/ml in the diagnostics of SQCLC was 0.68, specificity 0.090, positive predictive value 0.91, negative predictive value 0.65. Sensitivity of CEA by cut-off 4.61 ng/ml was 0.5 by the same specificity 0.90. CYFRA 21-1 has high sensitivity, specificity and positive predictive value in the diagnostics of SQCLC. Sensitivity of CYFRA 21-1 is significantly higher than sensitivity of CEA in this settings. (author)

  18. Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective:To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Conclusion:This is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.

  19. Clinical value of CEA and CYFRA 21-1 for the differentiation of benign and malignant pleural effusion by Meta analysis

    International Nuclear Information System (INIS)

    Objective: Pleural effusion is a common clinical complication caused by many benign or malignant diseases. Many published papers presented that tumor-marker assays, such as carcinoembryonic antigen (CEA) and cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1), played a vital role on the benign and malignant differentiation. This study aimed to reviewe currently available published papers to evaluate the clinical value of these two markers for the differentiation of benign and malignant pleural effusion. Methods: All published data of pleural effusion CEA and CYFRA 21-1 for the differentiation studies from year 1996 to 2007 were reviewed. By methodological qualities and Q test, 19 papers were selected into this study. The overall diagnostic sensitivity, specificity, and area under curve (AUC) from receiver operating characteristic (ROC) curve were adopted to compare the value of pleural effusion CEA and CYFRA 21-1 for benign and malignant differentiation used Z test. Results: There were 3228 subjects included in this Meta-analysis. The sensitivity and specificity [95% confidence interval (CI)] of CEA were 45.9% (43.2%-48.5%) and 97.0% (96.0%-97.8%), and of CYFRA 21-1, 47.3% (44.0%-50.6%) and 91.8% (89.5%-93.7%). AUC of CEA and CYFRA 21-1 were 0.7691 and 0.8213 respectively (Z=0.45, P>0.05). Conclusion: Both CEA and CYFRA 21-1 are useful for the differentiation of benign and malignant pleural effusion. (authors)

  20. Balance 2003 of the risks control at the Cea

    International Nuclear Information System (INIS)

    As a research center on the energy, the information and health technologies and the defense, the Cea activities are indissociable from the risk control notion. To organize the risks management, the Cea decided to create in july 2003 a special pole of risks control and management. This presentation is based on some major topics of the risks control: the environmental impact control, the occupational risks control, the installations safety control and the hazardous matter transport control. (A.L.B.)

  1. Detection of CEA mRNA on non-small lung cancer and it's significance

    Directory of Open Access Journals (Sweden)

    Guowen WANG

    2008-10-01

    Full Text Available Background and objective In recent years, many studies on micrometastasis in non-small cell lung cancer (NSCLC have been reported, this study is to investigate the effect of operation on micrometastasis from NSCLC andevaluate the relation between micrometastasis and clincopathological parameters. Methods The blood samples were taken from 70 cases of NSCLC and 18 patients with benign diseases at 3 intervals during the operation from peripheral vein. The transcription of carcinoembryonic antigen messenger ribonucleic acid (CEA mRNA was assayed by means of nested reverse transcriptase polymerase chain reaction (RT-PCR and micro-fluid chip. Results The CEA mRNA positive rates of all 3 time spots were as follows: 50% at beginning of the operation (Time 1, 62.8% at ligating the pulmonary vein (Time 2 and 57.1% at 1 h after ligating pulmonary vein (Time 3. There is significant difference between Time 1 and Time 2 (χ2=7.114, P <0.05. The positive rates of well-differentiation and middle-differentiation, stage Ⅰ and state Ⅱ, Tis, T1 and T2, N0 were significant less than non-differentiation and low-differentiation, stage Ⅲ and state Ⅳ, T3 and T4, N1,N2 and N3, respectively. No negative control samples was found to be positive, and no positive control samples was found to be negative. The sensitivity of our test was 10 cells/mL. Conclusion The cancer cells dissemination during operation was demonstrated indirectly in our study, the time of pulmonary vein ligation (earlier or later may affect the quantity of tumor cells released into circulation; The patients with lower differentiation, advanced TNM stage, larger tumor size and metastasis of lymph node have higher rates of metastasis in peripheral, so the detection of CEA mRNA can guide the therapy of NSCLC to a certain extent.

  2. A Carcinoembryonic Antigen-Secreting Adenocarcinoma Arising in Tailgut Cyst : Clinical Implications of Carcinoembryonic Antigen

    OpenAIRE

    Cho, Byoung Chul; Kim, Nam Kyu; Lim, Beom Jin; Kang, Sang Ook; Sohn, Ju Hyuk; Roh, Jae Kyung; Choi, Sang Tae; Kim, Sung Ai; Park, Se Eun

    2005-01-01

    Tailgut cysts (TGCs) are rare congenital cysts that occur in the retrorectal or presacral spaces. Although most tailgut cysts have been reported as benign, there have been at least 9 cases associated with malignant change. We report herein on an unusual case of a 40-year-old woman with a carcinoembryonic antigen (CEA)-producing adenocarcinoma arising within a TGC who underwent surgical resection and local radiation therapy. Despite the complete resection, metastatic adenocarcinoma developed f...

  3. Kinetics of anti-carcinoembryonic antigen antibody internalization: effects of affinity, bivalency, and stability

    Science.gov (United States)

    Schmidt, Michael M.; Thurber, Greg M.

    2010-01-01

    Theoretical analyses suggest that the cellular internalization and catabolism of bound antibodies contribute significantly to poor penetration into tumors. Here we quantitatively assess the internalization of antibodies and antibody fragments against the commonly targeted antigen carcinoembryonic antigen (CEA). Although CEA is often referred to as a non-internalizing or shed antigen, anti-CEA antibodies and antibody fragments are shown to be slowly endocytosed by LS174T cells with a half-time of 10–16 h, a time scale consistent with the metabolic turnover rate of CEA in the absence of antibody. Anti-CEA single chain variable fragments (scFvs) with significant differences in affinity, stability against protease digestion, and valency exhibit similar uptake rates of bound antibody. In contrast, one anti-CEA IgG exhibits unique binding and trafficking properties with twice as many molecules bound per cell at saturation and significantly faster cellular internalization after binding. The internalization rates measured herein can be used in simple computational models to predict the microdistribution of these antibodies in tumor spheroids. PMID:18408925

  4. Fusion technology. Annual report of the. Association Cea/EURATOM

    International Nuclear Information System (INIS)

    In 1996, the French EURATOM-CEA Association made significant contributions to the European technology programme. This work is compiled in this report as follows: the ITER CEA activities and related developments are described in the first section; blankets and material developments for DEMO, long term safety studies are summarised in the second part; the Underlying Technology activities are compiled in the third part of this report. In each section, the tasks are sorted out to respect the European presentation. For an easy reading, appendix 4 gives the list of tasks in alphabetical order with a page reference list. The CEA is in charge of the French Technology programme. Three specific organizational directions of the CEA, located on four sites (see appendix 5) are involves in this programme: Advanced Technologies Direction (DTA), for Material task; Nuclear Reactors Direction (DRN), for Blanket design, Neutronic problems, Safety tasks; Physical Sciences Direction (DSM) uses the competence of the Tore Supra team in the Magnet design and plasma Facing Component field. The CEA programme is completed by collaborations with Technicatome, COMEX-Nucleaire and Ecole Polytechnique. The breakdown of the programme by Directions is presented in figure 1. The allocation of tasks is given in appendix 2 and in appendix 3, the related publications. (author)

  5. Clinical Value of Serum HMGB1 Levels in Early Detection of Recurrent Squamous Cell Carcinoma of Uterine Cervix: Comparison with Serum SCCA, CYFRA21-1, and CEA Levels

    OpenAIRE

    Sheng, Xiugui; Du, Xuelian; Zhang, Xiaoling; Li, Dapeng; Lu, Chunhua; Li, Qinshui; Ma, Zhifang; Song, Quqing; Wang, Cong

    2009-01-01

    Aim To evaluate the clinical value of serum high mobility group box chromosomal protein 1 (HMGB1) levels in making the early diagnosis of recurrent cervical squamous cell carcinomas (CSCC) and compare it with the value of serum squamous cell carcinoma antigen (SCCA), cytokeratin fragment (CYFRA) 21-1, and carcinoembryonic antigen (CEA) levels. Methods Immunohistochemical staining of tissue from 64 patients with recurrent CSCCs, 72 patients with non-recurrent carcinoma,...

  6. A Case of Radiation Fibrosis Appearing as Mass-Like Consolidation after SBRT with Elevation of Serum CEA

    Directory of Open Access Journals (Sweden)

    Kotaro Terashima

    2010-01-01

    Full Text Available We report a case of radiation fibrosis appearing as mass-like consolidation, which was difficult to distinguish from local recurrence. A 72-year-old woman was diagnosed as having primary lung cancer (cT1N0M0 stage IA in the right upper lobe and was treated with SBRT of 48 Gy in 4 fractions. After 12 months, mass-like consolidation appeared around the irradiated area, and after 13 months, it had increased in size. FDG-PET revealed high uptake (SUV max=5.61 for the consolidation. CT-guided biopsy was performed, but we could not confirm the diagnosis. Considering her poor respiratory function and her age, short-interval follow-up was performed. After 15 months, the consolidation enlarged at the dorsal side, and carcinoembryonic antigen (CEA became elevated (14.6 ng/mL. Serum KL-6 (436 U/mL and SP-D (204 ng/mL were also elevated. However, after 16 months, serum CEA slightly decreased. The consolidation gradually retracted on follow-up CT images. CEA, KL-6, and SP-D were also decreased by degrees. After 40 months, there is no evidence of local recurrence.

  7. 2nd CEAS Specialist Conference on Guidance, Navigation and Control

    CERN Document Server

    Mulder, Bob; Choukroun, Daniel; Kampen, Erik-Jan; Visser, Coen; Looye, Gertjan

    2013-01-01

    Following the successful 1st CEAS (Council of European Aerospace Societies) Specialist Conference on Guidance, Navigation and Control (CEAS EuroGNC) held in Munich, Germany in 2011, Delft University of Technology happily accepted the invitation of organizing the 2nd  CEAS EuroGNC in Delft, The Netherlands in 2013. The goal of the conference is to promote new advances in aerospace GNC theory and technologies for enhancing safety, survivability, efficiency, performance, autonomy and intelligence of aerospace systems using on-board sensing, computing and systems. A great push for new developments in GNC are the ever higher safety and sustainability requirements in aviation. Impressive progress was made in new research fields such as sensor and actuator fault detection and diagnosis, reconfigurable and fault tolerant flight control, online safe flight envelop prediction and protection, online global aerodynamic model identification, online global optimization and flight upset recovery. All of these challenges de...

  8. Selection of aptamers to Anti-CEA%癌胚抗原特异性单克隆抗体(Anti-CEA)核酸适配体的筛选

    Institute of Scientific and Technical Information of China (English)

    曾家豫; 万波; 马瑾; 袁红霞; 李星; 赵运旺; 廖世奇

    2015-01-01

    目的:筛选癌胚抗原(carcinoembryonic antigen,CEA)特异性单克隆抗体(Anti-CEA)的核酸适配体(aptamers),为肺癌血清肿瘤标志物核酸适配体的筛选奠定实验基础.方法:利用羧基化琼脂磁珠作为筛选介质,以Anti-CEA为筛选的目标靶分子,通过消减SELEX技术及实时定量PCR技术,从随机ssDNA文库中筛选出与Anti-CEA特异性结合的aptamers,并通过凝胶阻滞实验(EMSA)鉴定筛选到的Anti-CEA-aptamer复合物,然后将得到的第10轮富集文库扩增为双链DNA,通过切胶纯化后,连接PMD18-T载体,转化大肠杆菌DH5α感受态细胞,同时利用交错PCR技术鉴定阳性克隆,经测序,获得aptamers的序列.结果:经过10轮筛选得到了4条与Anti-CEA结合的aptamers,测序结果显示均为不同序列.结论:验证筛选出的与Anti-CEA结合的aptamer,特异性检测结果表明2号aptamer与靶分子结合的特异性很高,且与非特异蛋白无明显吸附,筛选出的aptamers用于识别Anti-CEA,将为肺癌的早期诊断和早期治疗提供新的突破口.

  9. CEA Bolometer Arrays: the First Year in Space

    OpenAIRE

    Billot, Nicolas; Sauvage, M.; RODRIGUEZ, L.; Horeau, B.; Kiss, C.; Aussel, H.; Okumura, K; O. Boulade; Altieri, B.; Poglitsch, A.; Agnese, P.

    2010-01-01

    The CEA/LETI and CEA/SAp started the development of far-infrared filled bolometer arrays for space applications over a decade ago. The unique design of these detectors makes possible the assembling of large focal planes comprising thousands of bolometers running at 300 mK with very low power dissipation. Ten arrays of 16x16 pixels were thoroughly tested on the ground, and integrated in the Herschel/PACS instrument before launch in May 2009. These detectors have been successfully commissioned ...

  10. Remote manipulators and mobile robots in the CEA

    International Nuclear Information System (INIS)

    Thanks to Jean Vertut's works in the seventies, the CEA has been a pioneer in nuclear remote manipulation and mobile robots conception and has developed innovating systems in response to industry request. During the nineties, improvements in computer control systems have led to the realization of functional demonstrators. In 1995, advances in technology have allowed to consider the first industrial applications of nuclear remote manipulators and robots. This paper gives a review of the development and research works carried out by the CEA in this domain since the seventies, and also some future prospects. (J.S.). 10 figs

  11. Value of {sup 18}F-FDG uptake on PET/CT and CEA level to predict epidermal growth factor receptor mutations in pulmonary adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Kai-Hsiung; Hsu, Hsian-He; Chang, Wei-Chou; Hsu, Yi-Chih; Chang, Tsun-Hou [Tri-Service General Hospital and National Defense Medical Center, Department of Radiology, Taipei 114 (China); Huang, Tsai-Wang; Chang, Hung [Tri-Service General Hospital and National Defense Medical Center, Department of Thoracic Surgery, Taipei (China); Gao, Hong-Wei [Tri-Service General Hospital and National Defense Medical Center, Department of Pathology, Taipei (China); Shen, Daniel H.Y. [Tri-Service General Hospital and National Defense Medical Center, Department of Nuclear medicine, Taipei (China); Chu, Chi-Ming [Institute of Public Health, National Defense Medical Center and University, Section of Health Informatics, Taipei (China); Ho, Ching-Liang [Tri-Service General Hospital and National Defense Medical Center, Division of Hematology-Oncology, Department of Internal Medicine, Taipei (China)

    2014-10-15

    The identification of the mutation status of the epidermal growth factor receptor (EGFR) is important for the optimization of treatment in patients with pulmonary adenocarcinoma. The acquisition of adequate tissues for EGFR mutational analysis is sometimes not feasible, especially in advanced-stage patients. The aim of this study was to predict EGFR mutation status in patients with pulmonary adenocarcinoma based on {sup 18}F-fluorodeoxyglucose (FDG) uptake and imaging features in positron emission tomography/computed tomography (PET/CT), as well as on the serum carcinoembryonic antigen (CEA) level. We retrospectively reviewed 132 pulmonary adenocarcinoma patients who underwent EGFR mutation testing, pretreatment FDG PET/CT and serum CEA analysis. The associations between EGFR mutations and patient characteristics, maximal standard uptake value (SUVmax) of primary tumors, serum CEA level and CT imaging features were analyzed. Receiver-operating characteristic (ROC) curve analysis was performed to quantify the predictive value of these factors. EGFR mutations were identified in 69 patients (52.2 %). Patients with SUVmax ≥6 (p = 0.002) and CEA level ≥5 (p = 0.013) were more likely to have EGFR mutations. The CT characteristics of larger tumors (≥3 cm) (p = 0.023) and tumors with a nonspiculated margin (p = 0.026) were also associated with EGFR mutations. Multivariate analysis showed that higher SUVmax and CEA level, never smoking and a nonspiculated tumor margin were the most significant predictors of EGFR mutation. The combined use of these four criteria yielded a higher area under the ROC curve (0.82), suggesting a good discrimination. The combined evaluation of FDG uptake, CEA level, smoking status and tumor margins may be helpful in predicting EGFR mutation status in patients with pulmonary adenocarcinoma, especially when the tumor sample is inadequate for genetic analysis or genetic testing is not available. Further large-scale prospective studies are

  12. Biliary carcinoembryonic antigen levels in diagnosis of occult hepatic metastases from colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Jaques Waisberg; Rog(e)rio T. Palma; Lu(i)s Contim Neto; Lourdes C. Martins; Maur(i)cio S. L. Oliveira; Carlos A. Nagashima; Antonio C. Godoy; Fabio S. Goffi

    2003-01-01

    AIM: To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patients with colorectal carcinoma and the morphological and clinical features of neoplasia and the occurrence of hepatic metastases.METHODS: CEA levels in the gallbladder and peripheral blood were studied in 44 patients with colorectal carcinoma and 10 patients with uncomplicated cholelithiasis. CEA samples were collected from the gallbladder bile and peripheral blood during the operation, immediately before extirpating the colorectal neoplasia or cholecystectomy.Values of up to 5 ng/ml were considered normal for bile and serum CEA.RESULTS: In the 44 patients with colorectal carcinoma who underwent operation with curative intent, the average level of serum CEA was 8.5 ng/ml (range: 0.1 to 111.0 ng/ml) and for bile CEA it was 74.5 ng/ml (range: 0.2 to 571.0ng/ml). In the patients with uncomplicated cholelithiasis who underwent cholecystectomy, the average level of serum CEA was 1.9 ng/ml (range: 1.0 to 3.5 ng/ml) and for bile CEA it was 1.2 ng/ml (range: 0.3 to 2.9 ng/ml).The average duration of follow-up time was 16.5 months (range: 6 to 48 months). Four patients who underwent extirpation of the colorectal carcinoma without evidence of hepatic metastasis and with an average bile CEA value of 213.2 ng/ml presented hepatic metastases between three and seventeen months after removal of the primary colorectal neoplasia. Three of them successfully underwent extirpation of the hepatic lesions.CONCLUSION: High CEA levels in gallbladders of patients undergoing curative operation for colorectal carcinoma may indicate the presence of hepatic metastases. Such patients must be followed up with special attention to the diagnosis of such lesions.

  13. How Packaging Fleet Renewal Fits French CEA Programs

    International Nuclear Information System (INIS)

    CEA's (French Atomic Energy Agency) packaging fleet is dedicated to transportation of test irradiated fuels, of research reactors fuels, of navy propulsion fuels, and of waste coming from and to nuclear plants or facilities. This fleet encompasses more than 30 types of casks ranging from 5 to 30 tons, with either recent designs or other dating back to the seventies. A study has been launched in order to perform a global analysis of the life expectancy of the existing CEA and COGEMA Logistics cask fleets with respect to a 2015 target, in order to anticipate its renewal, while limiting the number of type of cask. Key elements like periodical evolutions of design and transport regulations, lessons learnt of existing casks (design, approval and extensions, operational feedback, maintenance and dismantling) are taken into account in order to ensure compliance and availability of the fleet. Moreover, from design to cask delivery, including regulatory tests, safety analysis report/ CoC, and manufacturing, 3 to 5 years is needed. Therefore cask development should be taken into account earlier of invest and research's programs. The paper will address the current life expectancy study of CEA and COGEMA Logistics packaging fleet, based on lessons learnt and regulation evolution and on general R and D plans by user facilities. It will show how a comprehensive optimized fleet is made available to CEA and other customers. Such a fleet combines optimized investment and uses, thus entailing synergies for well-mastered costs of transports. (authors)

  14. A sensitive label-free amperometric CEA immunosensor based on graphene-nafion nanocomposite film as an enhanced sensing platform.

    Science.gov (United States)

    Li, Yan; Yang, Wei-Kang; Fan, Man-Qi; Liu, Ao

    2011-01-01

    A novel approach to fabricate a label-free amperometric immunosensor for the detection of carcinoembryonic antigen (CEA) was described. Herein, methylene blue (MB), gold nanoparticles (AuNPs) and carcinoembryonic antibody (anti-CEA) were layer-by-layer assembled on the graphene-Nafion nanocomposite film-modified electrode by means of a self-assembling technique and the opposite-charged adsorption. Subsequently, the stepwise self-assembling procedure of the immunosensor was further characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors influencing the performance of the resulting immunosensor were studied in detail. The developed procedure showed improved features, including larger amount and higher immunoactivity of the immobilized antibody and repeatable regeneration of the sensor, as well as direct, rapid and simple determination for the antigen without multiple separation and labeling steps. The immunosensor could detect the target protein in a range of 0.5 to 120 ng/mL with a limit of 0.17 ng/mL (at 3σ). Finally, the immunosensing system was evaluated on several clinical samples. Analytical results were found to be in satisfactory agreement with those detected by the enzyme-linked immunosorbent assay (ELISA) method, indicating that this new method was a promising alternative tool for clinical diagnosis.

  15. Murine carcinoma expressing carcinoembryonic antigen-like protein is restricted by antibody against neem leaf glycoprotein.

    Science.gov (United States)

    Das, Arnab; Barik, Subhasis; Bose, Anamika; Roy, Soumyabrata; Biswas, Jaydip; Baral, Rathindranath; Pal, Smarajit

    2014-11-01

    We have generated a polyclonal antibody against a novel immunomodulator, neem leaf glycoprotein (NLGP) that can react to a specific 47 kDa subunit of NLGP. Generated anti-NLGP antibody (primarily IgG2a) was tested for its anti-tumor activity in murine carcinoma (EC, CT-26), sarcoma (S180) and melanoma (B16Mel) tumor models. Surprisingly, tumor growth restriction was only observed in CT-26 carcinoma models, without any alteration in other tumor systems. Comparative examination of antigenicity between four different tumor models revealed high expression of CEA-like protein on the surface of CT-26 tumors. Subsequent examination of the cross-reactivity of anti-NLGP antibody with purified or cell bound CEA revealed prominent recognition of CEA by anti-NLGP antibody, as detected by ELISA, Western Blotting and immunohistochemistry. This recognition seems to be responsible for anti-tumor function of anti-NLGP antibody only on CEA-like protein expressing CT-26 tumor models, as confirmed by ADCC reaction in CEA(+) tumor systems where dependency to anti-NLGP antibody is equivalent to anti-CEA antibody. Obtained result with enormous therapeutic potential for CEA(+) tumors may be explained in view of the epitope spreading concept, however, further investigation is crucial.

  16. Development of solid phase immunoradiometric assay for determination of carcinoembryonic antigen as a tumor marker

    International Nuclear Information System (INIS)

    Development of solid phase coated tube immunoradiometric assay for estimation of carcinoembryonic antigen (CEA) was the aim of the present study. Labeling of CEA was carried out using Ch-T and iodogen as oxidizing agents and 125I. The tracers were used to test the presence of antibodies produced by immunization. Production of polyclonal antibody was carried out through immunization of four mice. After purification step, the tubes were coated by purified polyclonal antibodies. Immunoradiometric that system was performed using the commercial IZOTOP 125I-anti hCEA tracer then the validity studies were carried out. The results show that the local coated tubes made the assay is more than sufficient to fulfill the clinical requirement of CEA as a tumor marker. (author)

  17. Iridium Oxide Film-Enhanced Impedance Immunosensor for Rapid Detection of Carcinoembyronic Antigen

    Institute of Scientific and Technical Information of China (English)

    DING,Yan-Jun; WANG,Hua; JIANG,Jian-Hui; SHEN,Guo-Li; YU,Ru-Qin

    2007-01-01

    A simple, rapid and sensitive impedance immunosensor based on iridium oxide (IrOx) thin film for the detection of carcinoembyronic antigen (CEA) in human sera has been proposed. Gold electrode was electrochemically modified with IrOx thin film and simultaneously functionalized with protein A (PA) to bind anti-CEA antibodies in an orientated way. It has been found that the antibody loading amount was dependent on the PA concentration and the deposition time of IrOx matrix. Under the optimized experimental conditions, the electron transfer resistances obtained were linearly related to the CEA concentration ranging from 36.2 to 460.0 ng/mL, with a detection limit of 28.0 ng/mL. Analytical results of clinical samples from cancer patients show that the proposed immunoassay is reasonably comparable with the chemiluminescence immunoassay (CLIA), indicating the feasibility of using the proposed method for CEA immunoassay in clinical laboratory.

  18. Evaluation of the nuclear installations safety of the CEA in 1998; Le bilan de la surete des installations nucleaires du CEA en 1998

    Energy Technology Data Exchange (ETDEWEB)

    Laverie, M. [CEA, Dir. de la Surete Nucleaire et de la Qualite, 75 - Paris (France)

    1999-09-01

    Michel Laverie, Director of the nuclear safety and quality at the Cea, took stoke of the CEA nuclear installations in 1998. After a recall of the nuclear safety policy and organization, the author presents the risks factors bound to the CEA activities as the dismantling, the wastes and the human factors. A last part is devoted to the list of the accidents occurred during 1998 in the nuclear installations. Tables and statistics illustrate this analysis. (A.L.B.)

  19. Annual report of the Association EURATOM/Cea

    International Nuclear Information System (INIS)

    This annual report presents research activities, which have been performed in 2002 by the French EURATOM-Cea association in the frame of the European technology program. The first section describes EFDA (European fusion development agreement) activities and related developments carried out by the association. The second one is dedicated to the underlying technology program and finally the third one presents the inertial confinement fusion activities. In each section the tasks are sorted out according to the EFDA main fields: physics (heating and current drive, remote participation, diagnostics), vessel/in-vessel (vessel/blanket, plasma facing components, remote handling), magnet, tritium breeding and materials (water cooled lithium lead blanket, helium cooled pebble bed blanket, helium cooled lithium lead blanket, reduced activation ferritic martensitic steels, advanced materials, neutron source, fuel cycle), safety and environment, system studies (power plant conceptual studies, socio-economic studies) and JET technology activities. The EURATOM-Cea association is involved in all these studies

  20. Overview of CEA research in the field of radionuclides migration

    International Nuclear Information System (INIS)

    This report presents a synthetic status of the researches conducted within the Nuclear Energy Division (CEA/DEN) in the field of radionuclides migration in three specific areas which have been chosen for their representativeness and potential impact: the migration of RN in PWR reactors, the migration of RN from a deep geological repository and the migration processes in the surface environments. In addition, some status is given about more generic research which is conducted in the field of RN speciation in the aqueous phase and at the interfaces and regarding chemistry / transport couplings. Additional information about the human and technical means involved in these fields of research in CEA/DEN is finally given in the Appendix. (authors)

  1. Annual report of the Association EURATOM/Cea

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, Ph.; Le Vagueres, F

    2002-07-01

    This annual report presents research activities, which have been performed in 2002 by the French EURATOM-Cea association in the frame of the European technology program. The first section describes EFDA (European fusion development agreement) activities and related developments carried out by the association. The second one is dedicated to the underlying technology program and finally the third one presents the inertial confinement fusion activities. In each section the tasks are sorted out according to the EFDA main fields: physics (heating and current drive, remote participation, diagnostics), vessel/in-vessel (vessel/blanket, plasma facing components, remote handling), magnet, tritium breeding and materials (water cooled lithium lead blanket, helium cooled pebble bed blanket, helium cooled lithium lead blanket, reduced activation ferritic martensitic steels, advanced materials, neutron source, fuel cycle), safety and environment, system studies (power plant conceptual studies, socio-economic studies) and JET technology activities. The EURATOM-Cea association is involved in all these studies.

  2. Carcinoembryonic Antigen Expression and Resistance to Radiation and 5-Fluorouracil-Induced Apoptosis and Autophagy.

    Science.gov (United States)

    Eftekhar, Ebrahim; Jaberie, Hajar; Naghibalhossaini, Fakhraddin

    2016-01-01

    Understanding the mechanism of tumor resistance is critical for cancer therapy. In this study, we investigated the effect of carcinoembryonic antigen (CEA) overexpression on UV-and 5-fluorouracil (5-FU)-induced apoptosis and autophagy in colorectal cancer cells. We used histone deacetylase (HDAC) inhibitor, NaB and DNA demethylating agent, 5-azacytidine (5-AZA) to induce CEA expression in HT29/219 and SW742 colorectal cancer cell lines. MTT assay was used to measure IC50 value of the cells exposed to graded concentrations of 5- FU with either 0.1 mM NaB or 1 μM 5-AZA for 72 h . Using CHO- and SW742-CEA transfectants, we also investigated the effect of CEA expression on UV- and 5-FU-induced apoptosis and autophagy. Treatment of HT29/219 cell line with NaB and 5-AZA increased CEA expression by 29% and 31%, respectively. Compared with control cells, the IC50 value for 5-FU of NaB and 5-AZA-treated cells increased by 40% and 57%, respectively. Treatment of SW742 cells with NaB or 5-AZA increased neither CEA expression nor the IC50 value for 5-FU. In comparison to parental cells, CEA expression also significantly protected transfected cells against UV-induced apoptosis. Decreased proportions of autophagy and apoptosis were also observed in 5-FU treated SW742- and CHO-CEA transfectants. We conclude that CEA expression can effectively protect colorectal cancer cells against radiation and drug-induced apoptosis and autophagy. PMID:27478804

  3. The CEA and alternative energies. 8 April 2010 press conference

    International Nuclear Information System (INIS)

    This document presents the CEA's strategy in terms of alternative energies and the various implemented research programs which mainly concern the building sector and the transport sector. After a recall of the energy and climate context, a presentation of the NTE program (Nouvelles Technologies de l'Energie, new energy technologies), the different topics and projects are presented: photovoltaic solar energy and its integration in building; batteries, hydrogen and fuel cells for applications in transports; second-generation bio-fuels

  4. Report transparency and nuclear safety 2007 CEA Cadarache

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Cadarache for the year 2007. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. More especially the report discusses the beginning of the RJH reactor construction, the fourth generation reactors research programs, the implementing of la Rotonde the new radioactive wastes management installation, the renovation of the LECA. (A.L.B.)

  5. Facilities offered by the CEA group for training foreign specialists

    International Nuclear Information System (INIS)

    First set up in 1945, the CEA (French Atomic Energy Commission) opened its doors to foreign scientists and technicians as long ago as the 1950s, and particularly so since 1960. Among the 15 000 foreigners concerned, some have come in the capacity of co-workers, while others - approximately 10 000 - have taken courses for the purpose of receiving training. Since the CEA constitutes both a public scientific institution and an industrial group, especially by virtue of its branch organizations and specialized production units, it possesses incomparably varied facilities. This is why almost all the countries of the world, at some time or other, have had nationals at the Saclay, Fontenay-aux-Roses, Grenoble and Cadarache research centres, or the National Institute of Nuclear Science and Technology (INSTN) at Saclay, a specialized establishment set up in 1956, or else at the Razes Information and Further Training Centre (CIPRA), specializing in the uranium ore industry. Most of the trainees received over the last few years by the CEA Group stem from the developing countries, those coming to receive practical technological training being ever more numerous. Several of the training programmes devised have involved participation in industrial operations, either in conjunction with Technicatome, a branch organization specializing in nuclear engineering (Iran, Iraq) or in association with other CEA Group units (South Korea). The trainees usually receive fellowships through the intermediary of French diplomatic missions, in which case it is enough if they continue to receive the salary normally paid to them by the public organization employing them. On the other hand, technological training sometimes involves payment because of the expense involved (intensive supervision and expensive experimental work). Finally, courses in French are organized in France by specialized institutions, but English is acceptable for very brief training periods. (author)

  6. Tumor Expression of the Carcinoembryonic Antigen Correlates with High Mitotic Activity and Cell Pleomorphism Index in Lung Carcinoma

    Directory of Open Access Journals (Sweden)

    Rancés Blanco

    2013-01-01

    Full Text Available At present, some research efforts are focusing on the evaluation of a variety of tumor associated antigens (TAAs for a better understanding of tumor biology and genetics of lung tumors. For this reason, we evaluated the tissue expression of carcinoembryonic antigen (CEA and ior C2 (a cell surface O-linked glycoprotein carbohydrate chain TAA in lung carcinomas, as well as its correlation with a variety of clinicopathological features. The tissue expression of CEA was evidenced in 22/43 (51.16% lung carcinomas and it was correlated with mitotic activity, cell pleomorphism indexes, and age of patients. The expression of ior C2 was observed in 15/43 (34.88% tumors but no correlation with the clinicopathological features mentioned above was obtained. No correlation between both CEA and ior C2 antigens expression and the overall survival (OS of non-small-cell lung cancer patients was also observed. However, CEA-negative patients displayed higher OS rates as compared with positive ones (69.74 versus 58.26 months. Our results seem to be in agreement with the role of CEA expression in tumor cell proliferation, inhibition of cell polarizations and tissue architecture distortion. The significance of ior C2 antigen in these malignancies and it potential use in diagnosis, prognosis, and/or immunotherapy must be reevaluated.

  7. CEA's research programmes on future nuclear power systems

    International Nuclear Information System (INIS)

    The CEA (Commissariat a l'energie atomique et aux energies alternatives) is at the crossroads between sciences and technologies. In collaboration with corporate leaders and academics, it is working to improve the competitiveness of the existing fleet of nuclear facilities (PWR reactors and EPR) and to develop both a fourth generation of nuclear power reactors and technologies for processing spent fuel. Concerning the PWR reactors most of the work focuses on the extension of reactor operating life (till 60 years), a better use of uranium fuel in the reactor core, a better safety standard, a better reliability of fuel assemblies. This work is both theoretical and experimental with the possibility to use irradiation rigs at the OSIRIS reactor. Concerning the EPR most of the work is dedicated to sustain the commissioning and start of the first EPR in France on the Flamanville site. Concerning the fuel cycle, CEA's teams are working to improve every stage of the cycle from uranium extraction to the processing of spent fuels. In the nuclear industry, industrial cycles are very long so it is today's work to prepare the fourth generation of reactors even if the first reactor of the third generation is not yet in operation. CEA's research focuses on 2 types of fast reactors, one is sodium-cooled (with ASTRID prototype) while the other is gas-cooled (with ALLEGRO prototype). (A.C.)

  8. Clinical Significance and Prognostic Value of CA72-4 Compared with CEA and CA19-9 in Patients with Gastric Cancer

    Directory of Open Access Journals (Sweden)

    M. Ychou

    2000-01-01

    Full Text Available Carcinoembryonic antigen (CEA and CA 19-9 are both widely used in the follow up of patients with gastrointestinal cancer. More recently another tumor marker, named CA 72-4 has been identified and characterized using two different monoclonal antibodies B72.3 and CC49. Several reports evaluated CA 72-4 as a serum tumor marker for gastric cancer and compared its clinical utility with that of CEA or CA 19-9; few reports concerned its prognostic value. In the present study, CA 72-4 is evaluated and compared with CEA and CA 19-9 in various populations of patients with gastric cancer and benign disease; for 52 patients with gastric adenocarcinoma and 57 patients without neoplastic disease CEA, CA 19-9 and CA 72-4 were evaluated before treatment. Sensitivity of the tumor markers CA 72-4, CA 19-9 and CEA at the recommended cut-off level in all 52 patients were 58%, 50% the sensitivity increased to 75%. of these markers, for non metastatic patients, multivariate analyses indicated that none of the markers were significant, when adjusted for gender and age (which were indicators of poor prognosis; patients with abnormal values of CA72-4 tended to have shorter survival than patients with normal values (p < 0.07. In the metastatic population, only high values of CA19-9 (p < 0.02 and gender (women (p < 0.03 were indicators of poor prognosis in univariate analysis; multivariate analysis revealed that both CA72-4 (p = 0.034 and CA19-9 p = 0.009, adjusted for gender were independent prognostic factors. However, CA72-4 lost significance (p = 0.41 when adjusted for CA19-9 and gender, indicating that CA19-9 provides more prognostic information than CA72-4.

  9. Radioimmunoscintigraphy of non-small cell lung cancer using Tc-99m ANTI-CEA FAB` (immuRAID CEA)

    Energy Technology Data Exchange (ETDEWEB)

    Fig, L.M. [DVA Medical Center, Ann Arbor, MI (United States); Hughes, L.; Pinsky, C.M. [Immunomedics Inc., Morris Plains, NJ (United States)] [and others

    1994-05-01

    We conducted a Phase II prospective multicenter clinical trial to investigate the safety and efficacy of Tc-99M anti-CEA Fab`(ImmuRAID-CEA) in patients with non-small cell lung cancer. Fifty four patients (42 male; 12 female; age range 40-93 yr) had primary operable (19), primary inoperable (7), occult (9), metastatic (15) or recurrent (4) disease. Patients were injected with 1 mg ImmuRAID-CEA radiolabeled with 20-30 mCi Tc-99m pertechnetate and imaged at 4-8 and 18-24 hr by planar and SPECT techniques; ten were imaged at 15-18 hr only. Despite prominent blood pool activity at 4-8 hr. overall imaging statistics on a per patient basis revealed a sensitivity of tumor detection of 90%, accuracy 85% and positive predictive value 94%. The later images had lower count rates but target to background ratios were improved. On a per lesion basis, sensitivity in the chest was 69% and in liver 88%. Twenty four patients had additional foci of antibody uptake at sites previously believed to be uninvolved; cancer was confirmed in 11, not confirmed in 2 and currently indeterminate in 11. Furthermore, a negative scan confirmed equivocal radiographic studies as true negative in 5 patients. With respect to safety, there were no adverse clinical reactions and four minor, transient changes in hematologic and/or biochemical parameters. Human anti-mouse antibody (HAMA) determinations on 27 patients using ImmuSTRIP{reg_sign} HAMA Fragment assay were negative at 4-6 weeks or 3 months post-infusion. We conclude that imaging with ImmuRAID-CEA is safe and potentially useful for the evaluation and staging of lung cancer patients.

  10. STAT3、CEA在人肺腺癌细胞A594中的相关性研究%Correlation of STAT3, CEA in lung adenocarcinoma cell A549

    Institute of Scientific and Technical Information of China (English)

    Debin Sun; Xiu Lan; Hongcheng Wang

    2012-01-01

    Objective: The purpose of this study was to analyze the relationship between signal transducer and activator of transcription 3 (STAT3) and carcinoembryonic antigen (CEA) in lung adenocarcinoma cell A549, and to explore the value of STAT3 on early diagnosis of lung adenocarcinoma. Methods: The expression of CEA, STAT3 mRNA and it's protein in human lung adenocarcinoma cell A549 and normal human lung cells MRC-5 were tested by immunohistochemistry staining (PV) and quantitative real time fluorescent PCR. The correlation between STAT3 and CEA in human lung adenocarcinoma cell A549 was analyzed. Results: The protein and mRNA levels of STAT3, CEA in lung adenocarcinoma cell A549 were apparently higher than those in normal human lung cells MRC-5. The levels of STAT3 mRNA and it's protein were positively correlated with CEA in lung adenocarcinoma cell A549. Conclusion: STAT3 have the same value in diagnosis of lung adenocarcinoma.

  11. Characterization of a common antigen of colorectal and mucinous ovarian tumors, COTA.

    Science.gov (United States)

    Pant, K D; Zamora, P O; Rhodes, B A; Sachatello, C R; Hagihara, P F; Griffen, W O; van Nagell, J R; Fulks, R; Ram, M D

    1984-01-01

    A new colon cancer antigen is reported. It is designated as COTA, Colon-Ovarian Tumor Antigen, because it is found in mucins produced by both tissues during malignancy. The new antigen was identified by making antibodies against human colon cancer tissue in goats. The antisera were exhaustively absorbed with lyophilized extracts of normal colon, lung, liver, spleen, kidney, plasma, and the well-known colon tumor antigen, carcinoembryonic antigen (CEA). The new antigen was identified by immunodiffusion. Studies of 28 malignant tissue extracts, 10 ovarian adenocarcinoma cyst fluids, 43 normal tissues, and 5 plasma samples revealed that this antigen is found only in colon tumors and mucinous ovarian adenocarcinomas. The antigen was not detected in serous adenocarcinoma of the ovaries, extracts of adenocarcinoma of lung, breast, kidney or stomach nor in the extracts of normal tissues. Other tests show that this antigen is not CEA, Ca 19-9, or CSAp. It is stable to heating at 65 degrees for 5 minutes; it elutes from an ion exchange matrix (DEAE) with 0.3-0.5M NaCl; it migrates to the alpha-2 region on immunoelectrophoresis; and its size, by exclusion chromatography on Sepharose 4B, is 3-15 million daltons. Anti-COTA stains colon cancer tissue sections indicating that COTA is present in goblet-cell mucin.

  12. Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA.

    Science.gov (United States)

    Tian, Lihui; Liu, Li; Li, Yueyuan; Wei, Qin; Cao, Wei

    2016-01-01

    A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and β-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001-100 ng/mL and a low detection limit of 0.27 pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays. PMID:27488806

  13. Diagnostic value of combined determination of serum tumor markers (NSE, CA-242, TPA, CEA) levels in patients with lung cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the diagnostic value of combined determination of serum NSE, CA242, tissue polypeptide antigen (TPA) and CEA levels in patients with primary lung cancer. Methods: Serum NSE, CA242, TPA and CEA levels were determined with ELISA in (1) 102 patients with various types of primary lung carcinoma (adenocarcinoma 38, squamous cell carcinoma 32, small cell lung carcinoma 32) (2) 33 patients with open lung T. B. and (3) 30 controls. Results: (1) In patients with lung cancer, serum levels of all the four markers were increased and significantly higher than their respective values in patients with open lung T.B. and controls. (2) Positive rate of combined any two markers were 75% for adenocarcinoma, 50% for squamous cell carcinoma and 65% for small cell lung carcinoma, while false positive rate was only 9% for T.B patients and none for the controls. (3) The most appropriate single marker for each specific type of lung cancer was: NSE for SCLC (sensitivity 72%, specificity 97%, CA242 for adenocarcinoma sensitivity 62%, specificity 90%). Conclusion: Combined determination of these tumor markers would improve the sensitivity and specificity for diagnosis of primary lung carcinoma. (authors)

  14. Ultrasensitive sandwich-type electrochemical immunosensor based on trimetallic nanocomposite signal amplification strategy for the ultrasensitive detection of CEA

    Science.gov (United States)

    Tian, Lihui; Liu, Li; Li, Yueyuan; Wei, Qin; Cao, Wei

    2016-01-01

    A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This immunosensor was developed by using the trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) nanosheets (NiAuPt-NGs) as excellent labels and β-cyclodextrin functionalized reduced graphene oxide nanosheets (CD-NGs) as the platform. The CD-NGs with high specific surface area good biocompatibility and the ideal dispersibility was used to capture the primary antibodies (Ab1) efficiently. The trimetallic NiAuPt-NGs nanocomposites were used as the labels for signal amplification, showing better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2), which is much better than that the monometallic Pt-NGs, bimetallic NiPt-NGs and AuPt-NGs due to the synergetic effect presented in NiAuPt-NGs. The NiAuPt-NGs nanocomposites consist of tightly coupled nanostructures of Au, Ni and Pt, which have neither an alloy nor a core-shell structure. Under the optimal conditions, a linear range from 0.001–100 ng/mL and a low detection limit of 0.27 pg/mL were obtained for CEA. The proposed electrochemical sandwich-type immunosensor may have a promising application in bioassay and it enriches the electrochemical immunoassays. PMID:27488806

  15. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

  16. The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran.

    Science.gov (United States)

    Heidari, Mohammad Hassan; Porghasem, Mohsen; Mirzaei, Nazanin; Mohseni, Jafar Hesam; Heidari, Matine; Azargashb, Eznollah; Movafagh, Abolfazl; Heidari, Reihane; Molouki, Aidin; Larijani, Leila

    2014-02-01

    Since several high level natural radiation areas (HLNRAs) exist on our planet, considerable attention has been drawn to health issues that may develop as the result of visiting or living in such places. City of Ramsar in Iran is an HNLRA, and is a tourist attraction mainly due to its hot spas. However, the growing awareness over its natural radiation sources has prompted widespread scientific investigation at national level. In this study, using an ELISA method, the level of expression of three tumor markers known as carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and carcino antigen 19-9 (CA19-9) in blood serum of 40 local men of Ramsar (subject group) was investigated and compared to 40 men from the city of Noshahr (control group). Noshahr was previously identified as a normal level natural radiation area (NLNRA) that is some 85 km far from Ramsar. According to statistical analysis, there was a significant difference in the levels of PSA and CA19-9 markers between the two groups (p < 0.001) with those of Ramsar being considerably higher. CEA level did not show any difference. Although some of the volunteers tested positive to the markers, they were in good health as confirmed by the physician. Moreover, the high number of positive markers in Noshahr was considerable. Therefore, future study is needed to further validate this result and to determine the level of positivity to tumor markers in both cities.

  17. Normal carcinoembryonic antigen indicates benefit from perioperative chemotherapy to gastric carcinoma patients

    Institute of Scientific and Technical Information of China (English)

    Shi Chen; Ying-Bo Chen; Yuan-Fang Li; Xing-Yu Feng; Zhi-Wei Zhou; Xiu-Hong Yuan; Chao-Nan Qian

    2012-01-01

    AIM:To evaluate pretreatment serum carcinoembryonic antigen (CEA) as a predictor of survival for patients with locally advanced gastric cancer receiving perioperative chemotherapy.METHODS:We retrospectively studied a cohort of 228gastric cancer patients who underwent D2 gastrectomy combined with chemotherapy at the Sun Yat-sen University Cancer Center between January 2005 and December 2009.Among them,168 patients received 6-12 cycles of oxaliplatin-based adjuvant (post-operative) chemotherapy,while 60 received perioperative chemotherapy (2 cycles of FOLFOX6 or XELOX before surgery and 4-10 cycles after surgery).Serum CEA was measured using an enzyme immunoassay.The followup lasted until December 2010.RESULTS:In the group that had elevated serum CEA,the difference in survival time between patients receiving perioperative chemotherapy and those receiving adjuvant chemotherapy had no statistical significance (P >0.05).However,in the group that had normal serum CEA,patients receiving perioperative chemotherapy had a longer survival time.In multivariate analysis,T staging and lymph node metastatic rate were independent prognostic factors for the patients.Perioperative chemotherapy improved the overall survival of patients who had a normal pretreatment CEA level (P =0.070).CONCLUSION:Normal pretreatment serum CEA is a predictor of survival for patients receiving perioperative chemotherapy.

  18. Aptasensor based on tripetalous cadmium sulfide-graphene electrochemiluminescence for the detection of carcinoembryonic antigen.

    Science.gov (United States)

    Shi, Gui-Fang; Cao, Jun-Tao; Zhang, Jing-Jing; Huang, Ke-Jing; Liu, Yan-Ming; Chen, Yong-Hong; Ren, Shu-Wei

    2014-11-21

    A facile label-free electrochemiluminescence (ECL) aptasensor, based on the ECL of cadmium sulfide-graphene (CdS-GR) nanocomposites with peroxydisulfate as the coreactant, was designed for the detection of carcinoembryonic antigen (CEA). Tripetalous CdS-GR nanocomposites were synthesized through a simple onepot solvothermal method and immobilized on the glassy carbon electrode surface. L-Cystine (L-cys) could largely promote the electron transfer and enhance the ECL intensity. Gold nanoparticles (AuNPs) were assembled onto the L-cys film modified electrode for aptamer immobilization and ECL signal amplification. The aptamer modified with thiol was adsorbed onto the surface of the AuNPs through a Au-S bond. Upon hybridization of the aptamer with the target protein, the sequence could conjugate CEA to form a Y architecture. With CEA as a model analyte, the decreased ECL intensity is proportional to the CEA concentration in the range of 0.01-10.0 ng mL(-1) with a detection limit of 3.8 pg mL(-1) (S/N = 3). The prepared aptasensor was applied to the determination of CEA in human serum samples. The recoveries of CEA in the human serum samples were between 85.0% and 109.5%, and the RSD values were no more than 3.4%. PMID:25209409

  19. Tissue Carcinoembryonic Antigen, Calcium, Copper and Iron Levels in Cancerous Lung Patients

    Directory of Open Access Journals (Sweden)

    Nasar Yousuf ALWAHAIBI

    2011-01-01

    Full Text Available Background and objective The expression of various trace elements and markers in lung cancer is controversial. The aim of this study is to evaluate the presence of calcium (Ca, copper (Cu, iron (Fe and carcinoembryonic antigen (CEA in cancerous untreated lung tissues and to determine a possible association between these markers and lung cancer. Methods Fourty-eight cancerous lung tissue blocks, from Sultan Qaboos University Hospital, Sultanate of Oman, were studied. Fe, Ca, Cu, and CEA were demonstrated in the tissue blocks using Perl's Prussian blue, Von Kossa's, modified rhodanine and immunohistochemical staining methods, respectively. Results Twenty-three of 48 specimens showed positive Fe staining, 2 showed positive Ca staining and Cu was absent in all specimens. 93.7% expressed CEA in varying degree of positivity. 81.25% of these sections showed high expression of CEA. Conclusion Tissue concentrations of trace elements were not elevated in lung cancer and therefore cannot be considered as a potential marker. Despite the low sensitivity and specificity of CEA as previously reported, tissue CEA should be considered as a potential marker in the evaluation of lung cancer.

  20. Tissue Carcinoembryonic Antigen, Calcium, Copper and Iron Levels in Cancerous Lung Patients

    Institute of Scientific and Technical Information of China (English)

    Nasar Yousuf ALWAHAIBI; Jokha Sultan ALGHARIBI; Amna Salim ALSHUKAILI; Ahmed Khalifa ALSHUKAILI

    2011-01-01

    Background and objective The expression of various trace elements and markers in lung cancer is controversial. The aim of this study is to evaluate the presence of calcium (Ca), copper (Cu), iron (Fe) and carcinoembryonic antigen (CEA) in cancerous untreated lung tissues and to determine a possible association between these markers and lung cancer.Methods Fourty-eight cancerous lung tissue blocks, from Sultan Qaboos University Hospital, Sultanate of Oman, were studied. Fe, Ca, Cu, and CEA were demonstrated in the tissue blocks using Perl's Prussian blue, Von Kossa's, modified rhodanine and immunohistochemical staining methods, respectively.Results Twenty-three of 48 specimens showed positive Fe staining, 2 showed positive Ca staining and Cu was absent in all specimens. 93.7% expressed CEA in varying degree of positivity. 81.25% of these sections showed high expression of CEA. Conclusion Tissue concentrations of trace elements were not elevated in lung cancer and therefore cannot be considered as a potential marker. Despite the low sensitivity and specificity of CEA as previously reported, tissue CEA should be considered as a potential marker in the evaluation of lung cancer.

  1. Synuclein gamma predicts poor clinical outcome in colon cancer with normal levels of carcinoembryonic antigen

    Directory of Open Access Journals (Sweden)

    Xing Xiaofang

    2010-07-01

    Full Text Available Abstract Background Synuclein gamma (SNCG, initially identified as a breast cancer specific gene, is aberrantly expressed in many different malignant tumors but rarely expressed in matched nonneoplastic adjacent tissues. In this study, we investigated the prognostic potential of SNCG in colon cancer particularly in the patients with normal carcinoembryonic antigen (CEA levels. Methods SNCG levels were assessed immunohistochemically in cancer tissues from 229 colon adenocarcinoma patients with a mean follow-up of 44 months. Correlations between SNCG levels and clinicopathologic features, preoperative serum CEA level, and clinical outcome were analyzed statistically using SPSS. Results SNCG levels in colon adenocarcinoma were closely associated with intravascular embolus and tumor recurrence but independent of preoperative serum CEA levels. SNCG expression was an independent prognostic factor of a shorter disease-free survival (DFS and overall survival (OS (P P = 0.001, P = 0.001, 0.002 for 97 patients with normal preoperative serum CEA level. Conclusions Our results suggest for the first time that SNCG is a new independent predicator for poor prognosis in patients with colon adenocarcinoma, including those with normal CEA levels. Combination of CEA with SNCG improves prognostic evaluation for patients with colon adenocarcinoma.

  2. Utility of slot-blot-ELISA as a new, fast, and sensitive immunoassay for detection of carcinoembryonic antigen in the urine samples of patients with various gastrointestinal malignancies.

    Science.gov (United States)

    El-Masry, Samir; El-Sayed, Ibrahim H; Lotfy, Mahmoud; Mahmoud, Lamiaa; El-Naggar, Mohamed

    2007-01-01

    Carcinoembryonic antigen (CEA) is the most widely used clinical tumor marker. CEA immunoassay has found acceptance as a diagnostic adjunct in clinical diagnosis of gastrointestinal tumors (GIT). Several immunoassays have been established for detection of CEA in plasma, serum, tissue, feces, and urine of cancer patients using polyclonal or monoclonal antibodies raised against CEA. Some of these assays display both high sensitivity and specificity for the detection of CEA. However, these assays require special and highly expensive equipment and the procedures require long periods for their completion. In the present study, we established a Slot-Blot Enzyme Linked Immunosorbent Assay (SB-ELISA), based on anti-CEA monoclonal antibody (CEA-mAb), as a new, simple, fast, cheap, and non-invasive immunodiagnostic technique for detection of CEA in the urine of GIT patients. Urine and serum samples were collected from 248 GIT patients (58 with pancreatic cancer, 20 with hepatoma, 23 with ampullary carcinoma, 15 with hilar cholangiocarcinoma, 28 with gastric cancer, 14 with esophageal cancer, and 90 with colorectal cancer). Moreover, urine and serum samples were collected from 50 healthy individuals to serve as negative controls. The traditional ELISA technique was used for determination of CEA in the sera of GIT patients using anti-CEA monoclonal antibody. A comparison between the results of both techniques (ELISA and SB-ELISA) was carried out. The traditional ELISA detected CEA in the sera of 154 out of 248 GIT patients with a sensitivity of 59.8%, 51.7% positive predictive value (PPV) and 75.37% negative predictive value (NPV). In addition, it identified 15 false positive cases out of 50 healthy individuals with a specificity of 70%. The urinary CEA was identified by a Western blotting technique and CEA-mAb at a molecular mass of 180 Kda. The developed SB-ELISA showed higher sensitivity, specificity, PPV, and NPV (70.1%, 78%, 62.4%, and 82.13%, respectively) for detection

  3. Poly(o-phenylenediamine) nanosphere-conjugated capture antibody immobilized on a glassy carbon electrode for electrochemical immunoassay of carcinoembryonic antigen

    International Nuclear Information System (INIS)

    We report on a new electrochemical immunosensor for the carcinoembryonic antigen (CEA; a model analyte). First, poly(o-phenylenediamine) nanospheres (PPDNSs) were synthesized by using a wet-chemistry method. The nanospheres were utilized as the support for immobilizing horseradish peroxidase-labeled polyclonal rabbit anti-human CEA antibody (HRP-anti-CEA) on a pretreated glassy carbon electrode (GCE) using glutaraldehyde as a crosslinker. In the presence of target CEA, an antigen-antibody immunocomplex formed on the electrode. This results in a partial inhibition of the active center of HRP and decreases the activity of HRP in terms of H2O2 reduction. The performance and factors influencing the performance of the immunoelectrode were studied. Under optimal conditions, the reduction current obtained from the anti-CEA-conjugated HRP (best at a working voltage of −265 mV vs. Ag/AgCl) is proportional to the CEA concentration in the 0.01 to 60 ng mL−1 range, with a detection limit of 3.2 pg mL−1. Non-specific adsorption was not observed. Relative standard deviations for intra-assay and inter-assay are <8.3 % and <9.7 %, respectively. The method was applied to the analysis of nine human serum samples, and a good relationship was found between the electrochemical immunoassay and the commercialized ELISA kit for human CEA. (author)

  4. Development of a double-antibody radioimmunoassay for detecting ovarian tumor-associated antigen fraction OCA in plasma

    International Nuclear Information System (INIS)

    Ovarian tumor-associated antigen isolated from human tumor tissue was shown to have a different mobility from that of carcinoembryonic antigen (CEA) in both acrylamide gel electrophoresis and immunoelectrophoresis in agarose. The ovarian tumor antigen is composed of six species with different electrophoretic mobility in acrylamide gel electrophoresis. Three of these species were detected in Sephadex G-100 ovarian fraction OCA (from the void volume peak) and the other three species of lower apparent molecular weight were detected in fraction OCD (from the second peak). Fractions OCA and OCD did not share common antigenic determinants as determined by immunodiffusion. CEA was shown to share antigenic determinants with both OCA and OCD. A double-antibody radioimmunoassay capable of detecting nanogram quantities of plasma OCA was developed. In a preliminary study of ovarian cancer patients, OCA appeared to be a more sensitive marker for ovarian cancer than CEA. There was virtually no correlation (r2 = 0.1) between OCA and CEA levels in these patients, as determined by radioimmunoassay

  5. Avaliação dos valores sérico e pleural dos marcadores tumorais CEA, CYFRA21-1 e CA 15-3 em portadores de derrame pleural Evaluation of serum and pleural levels of the tumor markers CEA, CYFRA21-1 and CA 15-3 in patients with pleural effusion

    Directory of Open Access Journals (Sweden)

    Isabella Coimbra Wagner

    2007-04-01

    Full Text Available OBJETIVO: Dosar os marcadores tumorais antígeno carcinoembrionário (CEA, fragmento da citoqueratina 19 (CYFRA21-1 e antígeno glicosídico associado a tumor 15-3 (CA 15-3 em sangue e líquido pleural de portadores de derrames pleurais benignos e malignos, avaliando a sensibilidade de cada um deles nesses fluidos. MÉTODOS: Avaliamos prospectivamente 85 pacientes com derrame pleural. O estudo do líquido pleural obedeceu a critérios determinados pela literatura. A dosagem dos marcadores foi realizada por eletroquimioluminescência. A sensibilidade foi determinada sob a condição de que a especificidade fosse > 90%. RESULTADOS: Foram diagnosticados 36 casos malignos (42,4%, 30 benignos (35,3%; em 19 pacientes (22,3%, o diagnóstico foi inconclusivo. Nos casos malignos, os valores de CEA e CYFRA21-1 foram maiores no líquido pleural do que no sangue, fato não observado para o CA 15-3. Nos casos benignos, os valores do CYFRA21-1 foram maiores no líquido pleural do que no soro, enquanto que para o CEA e o CA 15-3, ocorreu o oposto. Todos os marcadores apresentaram diferença significativa entre os casos malignos e benignos, em líquido pleural e soro. Foi encontrada sensibilidade para CEA, CYFRA21-1 e CA 15-3 no líquido pleural de 69,4%, 69,4% e 66,7%, respectivamente e quando associados, foi 80,6%. No soro, a sensibilidade foi 57,1, 71,4 e 48,6% para CEA, CYFRA21-1 e CA 15-3, respectivamente, e quando associados, foi 77%. CONCLUSÃO: Os resultados sugerem que a utilização desses marcadores pode ser útil na diferenciação entre derrames pleurais malignos e benignos.OBJECTIVE: To determine the levels of the tumor markers carcinoembryonic antigen (CEA, cytokeratin 19 fragment (CYFRA21-1 and carbohydrate antigen 15-3 (CA 15-3 in the blood and pleural fluid of patients with benign or malignant pleural effusion, evaluating the sensitivity of each marker in these fluids. METHODS: We prospectively evaluated 85 patients with pleural effusion. The

  6. Combined effects of 5-Fluorouracil, Folinic acid and Oxaliplatin on the expression of carcinoembryonic antigen in human colon cancer cells: pharmacological basis to develop an active antitumor immunochemotherapy

    Directory of Open Access Journals (Sweden)

    De Vecchis Liana

    2008-05-01

    Full Text Available Abstract Background Five-fluorouracil (FU, mainly associated with leucovorin (L, plays an essential role in chemotherapy of colorectal carcinoma. Moreover, FU ± L has been found to increase the expression of tumor-associated carcinoembryonic antigen (CEA, that may be an important target in therapeutic protocols of active specific immunotherapy. FU + L (FUL are frequently combined with oxaliplatin (OXA in advanced colon cancer patients. Thus, we investigated whether FUL in combination with OXA according to 2 different schedules may influence CEA expression in human colon cancer cells in vitro. Methods CEA protein expression was evaluated by cytofluorimetric and western blot analysis. Relative quantification of CEA mRNA was assessed by real time RT-PCR analysis. Results Levels of CEA protein and transcript were found to be higher in FUL-treated cells than in controls. However, when target cells were exposed to OXA before but not after FUL treatment, the up-regulation of CEA was partially inhibited. Conclusion These results suggest that target cells must be exposed to OXA after but not before treatment with the fluoropyrimidine in order to exploit drug-induced up-regulation of CEA. This finding appears to provide useful information to design chemo-immunotherapy protocols based on FUL + OXA, combined with host's immunity against CEA directed cancer vaccines.

  7. Preoperative serum levels of CEA and CA 242 in colorectal cancer.

    OpenAIRE

    Carpelan-Holmström, M; Haglund, C.; Kuusela, P.; Järvinen, H; Roberts, P. J.

    1995-01-01

    Preoperative serum levels of CEA and CA 242 were determined in 260 patients with colorectal cancer and in 92 patients with benign colorectal diseases. The overall sensitivity of the CEA test was 43% and of the CA 242 test 39%. The corresponding specificities were 90% and 87% respectively, using 5 ng ml-1 as cut-off level for CEA and 20 U ml-1 for CA 242. The sensitivity of CEA was 26%, 32%, 38% and 77% for Dukes A, B, C and D colorectal cancer, and the sensitivity of CA 242 was 26%, 26%, 40% ...

  8. CEA technical-economic activity report - Year 2014

    International Nuclear Information System (INIS)

    This report presents an assessment of activities undertaken by the CEA in the field of energy technique and economy during 2014. Technical-economic studies aims at contributing to national orientations on energy, at R and D program orientations, at highlighting and strengthening synergies between nuclear technologies and new energy technologies, and at strengthening the credibility of these technologies. After a presentation of the organisation of technique and economy within the CEA, of the involved bodies and departments, of the addressed themes (photovoltaic, wind, biomass and biofuels, hydrogen-based systems, mobility, electric systems and grids), the report presents studies performed in relationship with the national energy strategy and with energy scenarios, studies performed in the nuclear field (on fourth-generation reactors, on the front-end and back-end of the fuel cycle), studies performed in the field of new energy technologies and climate (hydrogen and storage, biofuels, climate, mobility, solar, electric systems). The next part addresses academic aspects, methodological studies and modelling studies. Contributions and participations to conferences, and publications are indicated

  9. 3rd CEAS Specialist Conference on Guidance, Navigation and Control

    CERN Document Server

    Drouin, Antoine; Roos, Clément

    2015-01-01

    The two first CEAS (Council of European Aerospace Societies) Specialist Conferences on Guidance, Navigation and Control (CEAS EuroGNC) were held in Munich, Germany in 2011 and in Delft, The Netherlands in 2013. ONERA The French Aerospace Lab, ISAE (Institut Supérieur de l’Aéronautique et de l’Espace) and ENAC (Ecole Nationale de l’Aviation Civile) accepted the challenge of jointly organizing the 3rd edition. The conference aims at promoting new advances in aerospace GNC theory and technologies for enhancing safety, survivability, efficiency, performance, autonomy and intelligence of aerospace systems. It represents a unique forum for communication and information exchange between specialists in the fields of GNC systems design and operation, including air traffic management. This book contains the forty best papers and gives an interesting snapshot of the latest advances over the following topics: l  Control theory, analysis, and design l  Novel navigation, estimation, and tracking methods l  Aircr...

  10. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer

    International Nuclear Information System (INIS)

    Cathepsin B and L (CATB, CATL), urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 play an important role in colorectal cancer invasion. The tumor marker utility and prognostic relevance of these proteases have not been evaluated in the same experimental setting and compared with that of CEA and CA-19-9. Protease, CEA and CA 19-9 serum or plasma levels were determined in 56 patients with colorectal cancer, 25 patients with ulcerative colitis, 26 patients with colorectal adenomas and 35 tumor-free control patients. Protease, CEA, CA 19-9 levels have been determined by ELISA and electrochemiluminescence immunoassay, respectively; their sensitivity, specificity, diagnostic accuracy have been calculated and correlated with clinicopathological staging. The protease antigen levels were significantly higher in colorectal cancer compared with other groups. Sensitivity of PAI-1 (94%), CATB (82%), uPA (69%), CATL (41%) were higher than those of CEA or CA 19-9 (30% and 18%, respectively). PAI-1, CATB and uPA demonstrated a better accuracy than CEA or CA 19-9. A combination of PAI-1 with CATB or uPA exhibited the highest sensitivity value (98%). High CATB, PAI-1, CEA and CA 19-9 levels correlated with advanced Dukes stages. CATB (P = 0.0004), CATL (P = 0.02), PAI-1 (P = 0.01) and CA 19-9 (P = 0.004) had a significant prognostic impact. PAI-1 (P = 0.001), CATB (P = 0.04) and CA 19-9 (P = 0.02) proved as independent prognostic variables. At the time of clinical detection proteases are more sensitive indicators for colorectal cancer than the commonly used tumor markers. Determinations of CATB, CATL and PAI-1 have a major prognostic impact in patients with colorectal cancer

  11. The value of F-18 fluorodeoxyglucose positron emission tomography/computed tomography in asymptomatic examinees with unexplained elevated blood carcinoembryonic antigen levels

    Energy Technology Data Exchange (ETDEWEB)

    Li, Wenfeng [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Radiation Oncology, Wenzhou (China); Yin, Weiwei [The First Affiliated Hospital of Wenzhou Medical University, Division of PET/CT, Department of Radiology, Wenzhou (China); Ou, Rongying [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Gynaecology and Obstetrics, Wenzhou (China); Chen, Ting; Xiong, Lingling; Xu, Yunsheng [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Dermatovenereology, Wenzhou (China); Cheng, Dezhi; Xie, Deyao [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Department of Cardiothoracic Surgery, Wenzhou (China); Zheng, Xiangwu; Zhao, Liang [The First Affiliated Hospital of Wenzhou Medical University, Laboratory for Advanced Interdisciplinary Research, Institutes of Translational Medicine, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Division of PET/CT, Department of Radiology, Wenzhou (China); The First Affiliated Hospital of Wenzhou Medical University, Institutes of Intelligent and Molecular Imaging, Wenzhou (China)

    2016-04-15

    Cancer is still a clinical challenge, with many efforts invested in order to achieve timely detection. Unexplained elevated blood carcinoembryonic antigen levels are occasionally observed in an asymptomatic population and considered as a risk factor of cancers. The purpose of this study was to determine the validity of 18 F-fluorodeoxyglucose-positron emission tomography/computed tomography (F-18 FDG-PET/CT) for detecting cancer in an asymptomatic population with an unexplained elevation in blood carcinoembryonic antigen (CEA) levels. This retrospective study included a total of 1920 asymptomatic examinees conducted from August 2011 through September 2013. The participants underwent CEA assay and conventional medical imaging (CEA-conventional), or CEA assay and F-18 FDG-PET/CT (CEA-PET/CT). The validity of conventional medical imaging and CEA-PET/CT scanning for detecting cancer and early-stage cancer in an asymptomatic population with an unexplained elevation in blood CEA levels were evaluated. Sensitivity, specificity, cancer detection rate, missed cancer detection rate, early-stage cancer detection rate, and early-stage cancer ratio using the CEA-PET/CT scanning were 96.6 %, 100 %, 10.4 %, 0.4 %, 3.7 %, and 34.5 %, respectively. In contrast, the corresponding values obtained using the conventional medical imaging were 50.6 % (P < 0.0001), 100 % (P > 0.9999), 50.6 % (P < 0.0001), 99.9 % (P = 0.055), 2.6 % (P < 0.0001), 2.5 % (P = 0.04), 0.7 % (P = 0.0004), and 14.5 % (P = 0.002), respectively. The F-18 FDG-PET/CT scanning significantly improved the validity of the cancer detection program in the asymptomatic population with an unexplained elevation in CEA levels. (orig.)

  12. The value of F-18 fluorodeoxyglucose positron emission tomography/computed tomography in asymptomatic examinees with unexplained elevated blood carcinoembryonic antigen levels

    International Nuclear Information System (INIS)

    Cancer is still a clinical challenge, with many efforts invested in order to achieve timely detection. Unexplained elevated blood carcinoembryonic antigen levels are occasionally observed in an asymptomatic population and considered as a risk factor of cancers. The purpose of this study was to determine the validity of 18 F-fluorodeoxyglucose-positron emission tomography/computed tomography (F-18 FDG-PET/CT) for detecting cancer in an asymptomatic population with an unexplained elevation in blood carcinoembryonic antigen (CEA) levels. This retrospective study included a total of 1920 asymptomatic examinees conducted from August 2011 through September 2013. The participants underwent CEA assay and conventional medical imaging (CEA-conventional), or CEA assay and F-18 FDG-PET/CT (CEA-PET/CT). The validity of conventional medical imaging and CEA-PET/CT scanning for detecting cancer and early-stage cancer in an asymptomatic population with an unexplained elevation in blood CEA levels were evaluated. Sensitivity, specificity, cancer detection rate, missed cancer detection rate, early-stage cancer detection rate, and early-stage cancer ratio using the CEA-PET/CT scanning were 96.6 %, 100 %, 10.4 %, 0.4 %, 3.7 %, and 34.5 %, respectively. In contrast, the corresponding values obtained using the conventional medical imaging were 50.6 % (P < 0.0001), 100 % (P > 0.9999), 50.6 % (P < 0.0001), 99.9 % (P = 0.055), 2.6 % (P < 0.0001), 2.5 % (P = 0.04), 0.7 % (P = 0.0004), and 14.5 % (P = 0.002), respectively. The F-18 FDG-PET/CT scanning significantly improved the validity of the cancer detection program in the asymptomatic population with an unexplained elevation in CEA levels. (orig.)

  13. Scientific evaluation at the French Atomic Energy Commission (CEA). 2005-2006 annual report; L'evaluation scientifique au CEA. Rapport annuel 2005-2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This report aims at presenting the scientific evaluation activities carried out at the CEA during the years 2005-2006. The evaluation system is described in detail. It comprises two aspects: the evaluation of the scientific policy implemented by the scientific Council and by the visiting committee of the CEA, and the evaluation of the laboratories, performed by a pool of 36 scientific councils. The evaluation by external and independent parties is the key point of this system. This document makes a status of the evaluations performed in 2005 and 2006. It presents a synthesis of the conclusions of the evaluation authorities. The actions implemented by the CEA to take into consideration the recommendations are also reported with the improvements noticed. The two topics examined by the scientific Committee and by the visiting committee were dealing with the energy domain, which is a strategic issue for the CEA. The examination of the researches on future nuclear reactors and on new energy technologies have shown the major role played by the CEA in the recent advances in these domains. About 95% of the laboratories activity was examined during the 2002-2005 period. The richness of the remarks and recommendations made by the scientific councils should allow the CEA to improve the quality and relevance of its research works. The start-up of the 2006-2009 evaluation cycle has been the occasion to modify the evaluation of some research domains in order to take into account the evolution of programs. The evaluation system of the CEA is highly consistent with the AERES principles. Its implementation, adapted to each type of activity (fundamental research, applied research, technological developments) allows the CEA to follow up a permanent improvement approach. (J.S.)

  14. Levels of estrogen, carcinoembryonic antigen and cancer antigen of breast in breast cancer patients

    International Nuclear Information System (INIS)

    This study was conducted during the period from february 2004 to July 2004; with the objective of measuring the levels of estrogen (E2), carcinoembryonic antigen (CEA) and cancer antigen of breast (CA-15.3) so as to facilitate the early diagnosis of breast cancer and determine the involvement of these parameters as risk factors for breast cancer. Ninety blood samples were collected from Sudanese females, divided into two groups; control group and patient groups. The patients group was sixty Sudanese females visiting the Radio Isotope Center, Khartoum (RICK) and they were confirmed as breast cancer patient by histopathology. The levels of the above mentioned parameters were determined by using radioimmunoassay technique. The results showed that, no significant (p=0.05) difference between the levels of the estrogen in patients compared to the control, on the other hand there was non significant (p>0.05) elevation in CEA levels in the patients with breast cancer compared to the control. The level of CA15.3 was significantly (p<0.0001) higher in the breast cancer patients compared to the control.(Author)

  15. Association of Neisseria gonorrhoeae Opa(CEA with dendritic cells suppresses their ability to elicit an HIV-1-specific T cell memory response.

    Directory of Open Access Journals (Sweden)

    Qigui Yu

    Full Text Available Infection with Neisseria gonorrhoeae (N. gonorrhoeae can trigger an intense local inflammatory response at the site of infection, yet there is little specific immune response or development of immune memory. Gonococcal surface epitopes are known to undergo antigenic variation; however, this is unlikely to explain the weak immune response to infection since individuals can be re-infected by the same serotype. Previous studies have demonstrated that the colony opacity-associated (Opa proteins on the N. gonorrhoeae surface can bind human carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1 on CD4⁺ T cells to suppress T cell activation and proliferation. Interesting in this regard, N. gonorrhoeae infection is associated with impaired HIV-1 (human immunodeficiency virus type 1-specific cytotoxic T-lymphocyte (CTL responses and with transient increases in plasma viremia in HIV-1-infected patients, suggesting that N. gonorrhoeae may also subvert immune responses to co-pathogens. Since dendritic cells (DCs are professional antigen presenting cells (APCs that play a key role in the induction of an adaptive immune response, we investigated the effects of N. gonorrhoeae Opa proteins on human DC activation and function. While morphological changes reminiscent of DC maturation were evident upon N. gonorrhoeae infection, we observed a marked downregulation of DC maturation marker CD83 when the gonococci expressing CEACAM1-specific Opa(CEA, but not other Opa variants. Consistent with a gonococcal-induced defect in maturation, Opa(CEA binding to CEACAM1 reduced the DCs' capacity to stimulate an allogeneic T cell proliferative response. Moreover, Opa(CEA-expressing N. gonorrhoeae showed the potential to impair DC-dependent development of specific adaptive immunity, since infection with Opa(CEA-positive gonococci suppressed the ability of DCs to stimulate HIV-1-specific memory CTL responses. These results reveal a novel mechanism to explain

  16. Association of Neisseria gonorrhoeae Opa(CEA) with dendritic cells suppresses their ability to elicit an HIV-1-specific T cell memory response.

    Science.gov (United States)

    Yu, Qigui; Chow, Edith M C; McCaw, Shannon E; Hu, Ningjie; Byrd, Daniel; Amet, Tohti; Hu, Sishun; Ostrowski, Mario A; Gray-Owen, Scott D

    2013-01-01

    Infection with Neisseria gonorrhoeae (N. gonorrhoeae) can trigger an intense local inflammatory response at the site of infection, yet there is little specific immune response or development of immune memory. Gonococcal surface epitopes are known to undergo antigenic variation; however, this is unlikely to explain the weak immune response to infection since individuals can be re-infected by the same serotype. Previous studies have demonstrated that the colony opacity-associated (Opa) proteins on the N. gonorrhoeae surface can bind human carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) on CD4⁺ T cells to suppress T cell activation and proliferation. Interesting in this regard, N. gonorrhoeae infection is associated with impaired HIV-1 (human immunodeficiency virus type 1)-specific cytotoxic T-lymphocyte (CTL) responses and with transient increases in plasma viremia in HIV-1-infected patients, suggesting that N. gonorrhoeae may also subvert immune responses to co-pathogens. Since dendritic cells (DCs) are professional antigen presenting cells (APCs) that play a key role in the induction of an adaptive immune response, we investigated the effects of N. gonorrhoeae Opa proteins on human DC activation and function. While morphological changes reminiscent of DC maturation were evident upon N. gonorrhoeae infection, we observed a marked downregulation of DC maturation marker CD83 when the gonococci expressing CEACAM1-specific Opa(CEA), but not other Opa variants. Consistent with a gonococcal-induced defect in maturation, Opa(CEA) binding to CEACAM1 reduced the DCs' capacity to stimulate an allogeneic T cell proliferative response. Moreover, Opa(CEA)-expressing N. gonorrhoeae showed the potential to impair DC-dependent development of specific adaptive immunity, since infection with Opa(CEA)-positive gonococci suppressed the ability of DCs to stimulate HIV-1-specific memory CTL responses. These results reveal a novel mechanism to explain why

  17. Report on transparency and nuclear safety 2014 - Cadarache CEA centre

    International Nuclear Information System (INIS)

    This document proposes, first, a presentation of the Cadarache CEA centre, of its activities and installations, gives a rather detailed overview of measures related to safety and to radiation protection within these activities and installations. Then it reports significant events related to safety and to radiation protection which occurred in 2014 and have been declared to the ASN. Next, it discusses the results of release measurements (liquid and gaseous effluents, radiological assessment, and chemical assessment for various installations) and the control of the chemical and radiological impact of these gaseous and liquid effluents on the environment. Finally, it addresses the issue of radioactive wastes which are stored in the different nuclear base installations of the Centre, indicates the different measures aimed at limiting the volume of these warehoused wastes and addresses their impact on health and on the environment. Nature and quantities of warehoused wastes are specified. Remarks and recommendations of the CHSCT are given

  18. Cryogenic turbulence test facilities at CEA/SBT

    Science.gov (United States)

    Rousset, B.; Baudet, C.; Bon Mardion, M.; Bourgoin, M.; Braslau, A.; Daviaud, F.; Diribarne, P.; Dubrulle, B.; Gagne, Y.; Gallet, B.; Gibert, M.; Girard, A.; Lehner, T.; Moukharski, I.; Sy, F.

    2015-12-01

    Recently, CEA Grenoble SBT has designed, built and tested three liquid helium facilities dedicated to turbulence studies. All these experiments can operate either in HeI or HeII within the same campaign. The three facilities utilize moving parts inside liquid helium. The SHREK experiment is a von Kármán swirling flow between 0.72 m diameter counterrotating disks equipped with blades. The HeJet facility is used to produce a liquid helium free jet inside a 0.200 m I.D., 0.47 m length stainless steel cylindrical testing chamber. The OGRES experiment consists of an optical cryostat equipped with a particle injection device and an oscillating grid. We detail specific techniques employed to accommodate these stringent specifications. Solutions for operating these facilities without bubbles nor boiling/cavitation are described. Control parameters as well as Reynolds number and temperature ranges are given.

  19. The stationary storage of energy. Available technologies and CEA researches

    International Nuclear Information System (INIS)

    After a discussion of the main challenges related to the stationary storage of energy, this publication proposes an overview of the different available technologies: plant for transfer of energy by pumping, compressed air, energy flywheels, hydrogen, lithium-ion battery, redox-flow battery, thermal storage by sensitive heat, thermal-chemical storage coupled to a thermal solar system, thermal storage by phase change, superconductive inductance storage, super-capacitors. It discusses the criteria of choice of storage technology, either for electric energy storage or for heat storage. It proposes an overview of researches performed within the CEA on storage systems: electrochemical, thermal, and hydrogen-based storages. The final chapter addresses current fundamental researches on storage in the field of lithium-ion batteries, hydrogen as a fuel, and thermoelectricity

  20. Planta de lavado para arena silícea

    OpenAIRE

    Rojo García, Cristina

    2008-01-01

    El proyecto fin de carrera trata sobre la instalación de una planta móvil de lavado para arena silícea. Este recurso está presente en el Monte Público nº 90 denominado “RAJICA DE EN MEDIO” en el Término Municipal de Jumilla (Murcia), donde se ubica la cantera “Los Mochuelos” de la que es concesionaria y explotadora la empresa JUMILLANA DE SÍLICE, S.L, con C.I.F. nº B-25.646.720, con domicilio social en C/ Canalejas nº 83, Bajo, de Jumilla (Murcia). Por ello, el presente proyecto tien...

  1. Development of a PMMA Electrochemical Microfluidic Device for Carcinoembryonic Antigen Detection

    Science.gov (United States)

    Van Anh, Nguyen; Van Trung, Hoang; Tien, Bui Quang; Binh, Nguyen Hai; Ha, Cao Hong; Le Huy, Nguyen; Loc, Nguyen Thai; Thu, Vu Thi; Lam, Tran Dai

    2016-05-01

    In this study, a poly(methyl methacrylate) (PMMA) microfluidic device fabricated by an inexpensive CO2 laser etching system was developed for detection of carcino-embryonic antigens (CEA). The device was capable of working in continuous mode and was designed with the aid of numerical simulation. The detection of target CEA was based on immuno-assay via magnetic particles and electrochemical sensing. The as-prepared microfluidic can be used to detect CEA at the relatively low concentration of 150 pg mL-1. The device could be reused many times, since the capture and removal of magnetic particles in the assay could be manipulated by an external magnetic field. The proposed approach appears to be suitable for high-throughput and automated analysis of large biomolecules such as tumor markers and pathogens.

  2. COTA (colon-ovarian tumor antigen). An immunohistochemical study.

    Science.gov (United States)

    Pant, K D; Fenoglio-Preiser, C M; Berry, C O; Zamora, P O; Ram, M D; Fulks, R M; Rhodes, B A

    1986-07-01

    A goat anti-serum was prepared against mucinous ovarian cyst fluid and absorbed with normal colon and a variety of normal tissues until the only residual immunoreactivity was directed against colon cancer and ovarian tumor mucin. The set of antigenic determinants defined by this anti-serum has been called COTA, standing for colon-ovarian-tumor-antigen. This highly absorbed anti-serum (anti-COTA) was used for immunohistochemical staining of 42 different tissues in parallel with staining with a goat anti-CEA, which was also highly absorbed. The results suggest that COTA is a highly sensitive and specific antigen for colon carcinoma and may have potential for the early detection of malignant changes predictive of cancer of the colon.

  3. Overview of the long-lived radionuclide separation processes developed in connection with the CEA`s spin programme

    Energy Technology Data Exchange (ETDEWEB)

    Madic, C.; Bourges, J.; Dozol, J.F.

    1994-12-31

    One possible strategy for eliminating the long-term potential hazards associated with the storage of vitrified high level waste produced by the processing of irradiated nuclear fuels, is the transmutation of the long-life radionuclides in these wastes into short-life radionuclides by nuclear means, which requires the prior chemical separation of these long-life radionuclides from the nuclear waste mixture. To do this, the French CEA (Atomic Energy Commissionership) launched the SPIN (Separation-Incineration) research programme in 1991, under the law passed by the parliament on december 30, 1991. Research currently under way to develop separation processes for some long-life radionuclides concerns modifications of the Purex process, and the development of new solvent extraction processes. The research directions are briefly summarized here. (authors). 17 refs., 3 tabs.

  4. Carcinoembryonic antigen-producing adrenal adenoma resected using combined lateral and anterior transperitoneal laparoscopic surgery

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A 74-year-old woman presented with symptoms consistent with hyperadrenocorticism and hyperca techolaminism. She had a cushingoid appearance and her cortisol level was elevated. Herserum dopamine and noradrenalin levels were also elevated.Computed tomography detected a left adrenal mass measuring 3.5 cm × 3.0 cm in diameter. Metaiodobenzylguanidine cintigraphy was negative. Unexpectedly, the serum Serum carcinoembryonic antigen (CEA) level was elevated.Fluorodeoxyglucose positron emission tomography showed increased uptake in the adrenal tumor only, with a maximum standardized uptake value of 2.8. Selective venography and blood sampling revealed that the concentrations of cortisol, catecholamines and CEA were significantly elevated in the vein draining the tumor. A diagnosis of CEA-produdng benign adenoma was made. After preoperative management, we performed a combined lateral and anterior transperitoneal laparoscopic adrenectomy. Her vital signs remained stable during surgery. Histopathological examination revealed a benign adenoma. Her cortisol, catecholamine and CEA levels normalized immediately after surgery. We present, to the best of our knowledge, the first case of CEA-roducin gadrenal adenoma, along with a review of the relevant literature, and discuss our laparoscopic surgery techniques.

  5. An ultrasensitive chemiluminescence biosensor for carcinoembryonic antigen based on autocatalytic enlargement of immunogold nanoprobes.

    Science.gov (United States)

    Hao, Minjia; Ma, Zhanfang

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl(4)- to Au0 on their surface in the presence of NH(2)OH·HCl. AuCl(4)-, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL-1 to 1,000 ng∙mL-1, with a detection limit of 20 pg∙mL-1. PMID:23443399

  6. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    Directory of Open Access Journals (Sweden)

    Minjia Hao

    2012-12-01

    Full Text Available A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA detection based on signal amplification with gold nanoparticles (NPs is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4− to Au0 on their surface in the presence of NH2OH·HCl. AuCl4−, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL−1 to 1,000 ng∙mL−1, with a detection limit of 20 pg∙mL−1.

  7. Increased basolateral sorting of carcinoembryonic antigen in a polarized colon carcinoma cell line after cholesterol depletion-Implications for treatment of inflammatory bowel disease

    Institute of Scientific and Technical Information of China (English)

    Robert Ehehalt; Markus Krautter; Martin Zorn; Richard Sparla; Joachim Fūllekrug; Hasan Kulaksiz; Wolfgang Stremmel

    2008-01-01

    AIM:To investigate a possible increase of basolateral expression of carcinoembryonic antigen(CEA)by interfering with the apical transport machinery,we studied the effect of cholesterol depletion on CEA sorting and secretion.METHODS:Cholesterol depletion was performed in polarized Caco-2 cells using Iovastatin and methyl-βcyclodextrin.RESULTS:We show that CEA is predominantly expressed and secreted at the apical surface.Reduction of the cholesterol level of the cell by 40%-50% with Iovastatin and methyl-β-cyclodextrin led to a significant change of the apical-to-basolateral transport ratio towards the basolateral membrane.CONCLUSION:As basolateral expression of CEA has been suggested to have anti-inflamatory properties,Cholesterol depletion of enterocytes might be a potential approach to influence the course of inflammatory bowel disease.

  8. Pre-clinical evaluation of a novel CEA-targeting near-infrared fluorescent tracer delineating colorectal and pancreatic tumors

    Science.gov (United States)

    Boonstra, Martin C.; Tolner, Berend; Schaafsma, Boudewijn E.; Boogerd, Leonora S.F.; Prevoo, Hendrica A.J.M; Bhavsar, Guarav; Kuppen, Peter J.K.; Sier, Cornelis F.M.; Bonsing, Bert A.; Frangioni, John V.; van de Velde, Cornelis J.H.; Chester, Kerry A.; Vahrmeijer, Alexander L.

    2016-01-01

    Surgery is the cornerstone of oncologic therapy with curative intent. However, identification of tumor cells in the resection margins is difficult, resulting in non-radical resections, increased cancer recurrence and subsequent decreased patient survival. Novel imaging techniques that aid in demarcating tumor margins during surgery are needed. Overexpression of carcinoembryonic antigen (CEA) is found in the majority of gastro-intestinal carcinomas, including colorectal and pancreas. We developed ssSM3E/800CW, a novel CEA-targeted near-infrared fluorescent (NIRF) tracer, based on a disulphide stabilized single-chain antibody fragment (ssScFv), to visualize colorectal and pancreatic tumors in a clinically translatable setting. The applicability of the tracer was tested for cell and tissue binding characteristics and dosing using immunohistochemistry, flow cytometry, cell-based plate assays and orthotopic colorectal (HT-29, well differentiated) and pancreatic (BXPC-3, poorly differentiated) xenogeneic human-mouse models. NIRF signals were visualized using the clinically compatible FLARE™ imaging system. Calculated clinically relevant doses of ssSM3E/800CW selectively accumulated in colorectal and pancreatic tumors/cells, with highest tumor-to-background ratios of 5.1±0.6 at 72 h post-injection, which proved suitable for intra-operative detection and delineation of tumor boarders and small (residual) tumor-nodules in mice, between 8 h and 96 h post-injection. Ex vivo fluorescence imaging and pathologic examination confirmed tumor-specificity and the distribution of the tracer. Our results indicate that ssSM3E/800CW shows promise as a diagnostic tool to recognize colorectal and pancreatic cancers for fluorescent-guided surgery applications. If successful translated clinically, this tracer could help improve the completeness of surgery and thus survival. PMID:25895046

  9. Biodistribution and tumor imaging of an anti-CEA single-chain antibody-albumin fusion protein

    Energy Technology Data Exchange (ETDEWEB)

    Yazaki, Paul J. [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States)], E-mail: pyazaki@coh.org; Kassa, Thewodros; Cheung, Chia-wei; Crow, Desiree M. [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States); Sherman, Mark A. [Division of Information Sciences, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States); Bading, James R.; Anderson, Anne-Line J.; Colcher, David; Raubitschek, Andrew [Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010 (United States)

    2008-02-15

    Albumin fusion proteins have demonstrated the ability to prolong the in vivo half-life of small therapeutic proteins/peptides in the circulation and thereby potentially increase their therapeutic efficacy. To evaluate if this format can be employed for antibody-based imaging, an anticarcinoembryonic antigen (CEA) single-chain antibody(scFv)-albumin fusion protein was designed, expressed and radiolabeled for biodistribution and imaging studies in athymic mice bearing human colorectal carcinoma LS-174T xenografts. The [{sup 125}I]-T84.66 fusion protein demonstrated rapid tumor uptake of 12.3% injected dose per gram (ID/g) at 4 h that reached a plateau of 22.7% ID/g by 18 h. This was a dramatic increase in tumor uptake compared to 4.9% ID/g for the scFv alone. The radiometal [{sup 111}In]-labeled version resulted in higher tumor uptake, 37.2% ID/g at 18 h, which persisted at the tumor site with tumor: blood ratios reaching 18:1 and with normal tissues showing limited uptake. Based on these favorable imaging properties, a pilot [{sup 64}Cu]-positron emission tomography imaging study was performed with promising results. The anti-CEA T84.66 scFv-albumin fusion protein demonstrates highly specific tumor uptake that is comparable to cognate recombinant antibody fragments. The radiometal-labeled version, which shows lower normal tissue accumulation than these recombinant antibodies, provides a promising and novel platform for antibody-based imaging agents.

  10. Fusion technology. Annual report of the. Association Cea/EURATOM; Technologie de fusion.Rapport annuel de l`association CEA/Euratom

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, P.; Le Vagueres, F.

    1996-12-31

    In 1996, the French EURATOM-CEA Association made significant contributions to the European technology programme. This work is compiled in this report as follows: the ITER CEA activities and related developments are described in the first section; blankets and material developments for DEMO, long term safety studies are summarised in the second part; the Underlying Technology activities are compiled in the third part of this report. In each section, the tasks are sorted out to respect the European presentation. For an easy reading, appendix 4 gives the list of tasks in alphabetical order with a page reference list. The CEA is in charge of the French Technology programme. Three specific organizational directions of the CEA, located on four sites (see appendix 5) are involves in this programme: Advanced Technologies Direction (DTA), for Material task; Nuclear Reactors Direction (DRN), for Blanket design, Neutronic problems, Safety tasks; Physical Sciences Direction (DSM) uses the competence of the Tore Supra team in the Magnet design and plasma Facing Component field. The CEA programme is completed by collaborations with Technicatome, COMEX-Nucleaire and Ecole Polytechnique. The breakdown of the programme by Directions is presented in figure 1. The allocation of tasks is given in appendix 2 and in appendix 3, the related publications. (author).

  11. Pharmacokinetics and Dosimetry Studies for Optimization of Pretargeted Radioimmunotherapy in CEA-Expressing Advanced Lung Cancer Patients

    Directory of Open Access Journals (Sweden)

    Caroline eBodet-Milin

    2015-11-01

    Full Text Available Objectives. A phase I pretargeted radioimmunotherapy trial (EudractCT 200800603096 was designed in patients with metastatic lung cancer expressing carcinoembryonic antigen (CEA to optimize bispecific antibody and labelled peptide doses, as well as the delay between their injections.Methods. Three cohorts of 3 patients received the anti-CEA x anti-histamine-succinyl-glycine (HSG humanized trivalent bispecific antibody (TF2 and the IMP288 bivalent HSG-peptide. Patients underwent a pre-therapeutic imaging session S1 (44 or 88 nmol/m2 of TF2 followed by 4.4 nmol/m2, 185 MBq, of 111In-labelled IMP288, and, 1-2 weeks later, a therapy session S2 (240 or 480 nmol/m2 of TF2 followed by 24 nmol/m2, 1.1 GBq/m2, 177Lu-labeled IMP288. The pretargeting delay was 24 or 48 hours. The dose schedule was defined based on pre-clinical TF2 pharmacokinetic studies, on our previous clinical data using the previous anti-CEA pretargeting system and on clinical results observed in the first patients injected using the same system in the Netherlands.Results. TF2 pharmacokinetics (PK was represented by a two-compartment model in which the central compartment volume was linearly dependent on the patient's surface area. PK were remarkably similar, with a clearance of 0.33 +/- 0.03 L/h per m2. 111In- and 177Lu-IMP288 PK were also well represented by a two-compartment model. IMP288 PK were faster (clearance 1.4 to 3.3 l/h. The central compartment volume was proportional to body surface area and IMP288clearance depended on the molar ratio of injected IMP288 to circulating TF2 at the time of IMP288 injection. Modelling of image quantification confirmed the dependence of IMP288 kinetics on circulating TF2, but tumour activity PK were variable. Organ absorbed doses were not significantly different in the 3 cohorts, but the tumour dose was significantly higher with the higher molar doses of TF2 (p < 0.002. S1 imaging predicted absorbed doses calculated in S2. Conclusion. The best

  12. Imaging of pharyngeal and laryngeal carcinomas with indium-111-labeled monoclonal anti-CEA antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Kairemo, K.J.; Hopsu, E.V. (Helsinki Univ. Central Hospital (Finland))

    1990-10-01

    Localization of primary tumors, metastases, or recurrences in 13 consecutive patients with histological verification of squamous cell or adenocarcinoma was made with radioimmunodetection using monoclonal radiolabeled anti-CEA antibody. All surgical specimens stained immunohistochemically, except one, were positive for CEA. Of the known 19 tumor sites 17 were visualized in antibody scans. There were two positive findings that did not prove to be positive during 12 month follow-up. The scintigram findings did not correlate with CEA serum concentrations that, with one exception, were normal in all patients.

  13. Correlation of the Serum Level of Carcinoembryonic Antigen and Prolactin with Different Stages of Colorectal Carcinoma According to Dukes' Staging.

    Science.gov (United States)

    Rahman, M R; Sheikh, S H; Lima, I J; Islam, M R; Faisal, M; Islam, M S; Faruk, M O; Jalal, M T

    2016-01-01

    Carcinoembryonic antigen (CEA) is well established tumor marker for colorectal cancers worldwide. Recent studies show that serum prolactin level is also raised in colorectal cancers. The purpose of the study is to evaluate the correlation of serum CEA and Prolactin with Dukes' staging of colorectal carcinomas. Between January 2013 and June 2013, Serum CEA and Serum Prolactin were measured by radioimmunoassay from 103 patients who were histopathologically diagnosed as colorectal carcinomas. Evaluation of the stages of the colorectal cancers was done on the basis of preoperative investigations and postoperative histopathology and correlated with Preoperative Serum CEA and Serum Prolactin. Results were presented as median value, range and percentage. Male to female ratio was 1.4:1 with median age of 42.26 years (range 17-78 years). Most of the patients in this series presented with carcinoma rectum (42%). Most of the patients (52%) were found in Dukes' stage C and 27% and 15% cases were found as Dukes' stage B and Dukes' stage D respectively. Stage of the disease is directly proportionate to percentage of the patient with high serum prolactin except early stage (Dukes' A-50%, Dukes' B-28.6%, Dukes' C-33.3% & Dukes' D-46.7%). Similarly serum CEA level is directly proportionate to tumor stage (Dukes' A-0%, Dukes' B-32%, Dukes' C-40.7% & Dukes' D-74.7%). A preoperative high serum CEA value suggests advanced disease either locally or with distant metastasis. In contrast preoperative high serum prolactin (hyperprolactinaemia) did not suggest advanced disease as it can be elevated even in early stage of disease. Serum CEA and Serum Prolactin both are valuable tumor markers but serum CEA could not be replaced by serum Prolactin. Serum Prolactin may be a helpful marker in earlier stages of the colorectal cancer.

  14. Evaluation of tumor markers carcinoembryonic antigen, cytokeratin 19 fragment and cancer-associated antigen 72-4 in neoplastic and non-neoplastic canine effusions differentiation

    OpenAIRE

    L.V Teixeira; T.A. Guerra; F.O. Conrado; S.R. Terra; D.G. Gerardi; González, F.H.D.

    2014-01-01

    The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1), cancer-associated antigen 72-4 (CA 72-4) and carcinoembryonic antigen (CEA) in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16) and non-neoplastic (n=16) samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistica...

  15. Improved cytotoxic T-lymphocyte immune responses to a tumor antigen by vaccines co-expressing the SLAM-associated adaptor EAT-2.

    Science.gov (United States)

    Aldhamen, Y A; Seregin, S S; Kousa, Y A; Rastall, D P W; Appledorn, D M; Godbehere, S; Schutte, B C; Amalfitano, A

    2013-10-01

    The signaling lymphocytic activation molecule-associated adaptor Ewing's sarcoma's-activated transcript 2 (EAT-2) is primarily expressed in dendritic cells, macrophages and natural killer cells. Including EAT-2 in a vaccination regimen enhanced innate and adaptive immune responses toward pathogen-derived antigens, even in the face of pre-existing vaccine immunity. Herein, we investigate whether co-vaccinations with two recombinant Ad5 (rAd5) vectors, one expressing the carcinoembryonic antigen (CEA) and one expressing EAT-2, can induce more potent CEA-specific cytotoxic T lymphocyte (CTL) and antitumor activity in the therapeutic CEA-expressing MC-38 tumor model. Our results suggest that inclusion of EAT-2 significantly alters the kinetics of Th1-biasing proinflammatory cytokine and chemokine responses, and enhances anti-CEA-specific CTL responses. As a result, rAd5-EAT2-augmented rAd5-CEA vaccinations are more efficient in eliminating CEA-expressing target cells as measured by an in vivo CTL assay. Administration of rAd5-EAT2 vaccines also reduced the rate of growth of MC-38 tumor growth in vivo. Also, an increase in MC-38 tumor cell apoptosis (as measured by hematoxylin and eosin staining, active caspase-3 and granzyme B levels within the tumors) was observed. These data provide evidence that more efficient, CEA-specific effector T cells are generated by rAd5 vaccines expressing CEA, when augmented by rAd5 vaccines expressing EAT-2, and this regimen may be a promising approach for cancer immunotherapy in general.

  16. BDDR, a new CEA technological and operating reactor database

    Energy Technology Data Exchange (ETDEWEB)

    Soldevilla, M.; Salmons, S.; Espinosa, B. [CEA-Saclay, CEA/DEN/DANS/DM2S/SERMA, 91191 Gif-sur-Yvette (France); Clanet, M.; Boudin, X. [CEA-Bruyeres-le-Chatel, 91297 Arpajon (France)

    2013-07-01

    The new application BDDR (Reactor database) has been developed at CEA in order to manage nuclear reactors technological and operating data. This application is a knowledge management tool which meets several internal needs: -) to facilitate scenario studies for any set of reactors, e.g. non-proliferation assessments; -) to make core physics studies easier, whatever the reactor design (PWR-Pressurized Water Reactor-, BWR-Boiling Water Reactor-, MAGNOX- Magnesium Oxide reactor-, CANDU - CANada Deuterium Uranium-, FBR - Fast Breeder Reactor -, etc.); -) to preserve the technological data of all reactors (past and present, power generating or experimental, naval propulsion,...) in a unique repository. Within the application database are enclosed location data and operating history data as well as a tree-like structure containing numerous technological data. These data address all kinds of reactors features and components. A few neutronics data are also included (neutrons fluxes). The BDDR application is based on open-source technologies and thin client/server architecture. The software architecture has been made flexible enough to allow for any change. (authors)

  17. CEA distribution transformer purchasing specifications (DTWG-01,02,03)

    International Nuclear Information System (INIS)

    Purchasing specifications for three types of distribution transformers are presented. The specifications were compiled by the Canadian Electricity Association at the suggestion made in 1989 by the Canadian Utilities Material Management Group. The specifications cover pole mounted single phase distribution transformers (DTWG-01), low-profile, single phase, dead-front pad-mounted distribution transformers (DTWG-02), and three phase, dead-front pad-mounted distribution transformers (DTWG-03). The specifications were compiled by a task force of CEA member utilities, using CSA standards as the governing standards in all three cases. The first edition of the purchasing specifications was issued in 1993. The second edition, consisting mainly of revisions based on experiences learned from using the first edition, and the addition of the appropriate clauses of CSA standards, was published in 1998. Based on a three-year average of the number of transformers purchased annually (about 58,000) at an estimated total cost of $ 120 million, use of the Purchasing Specifications is said to have resulted in savings of about 7 per cent or $ 8.4 million

  18. Report on transparency and nuclear safety - Cadarache CEA centre - 2012

    International Nuclear Information System (INIS)

    A first volume proposes a presentation of the Cadarache CEA centre, of its activities and installations, gives a rather detailed overview of measures related to safety and to radiation protection within these activities and installations. It also reports significant events related to safety and to radiation protection which occurred in 2012 and have been declared to the ASN. It discusses the results of release measurements (liquid and gaseous effluents, radiological assessment, and chemical assessment for various installations) and the control of the chemical and radiological impact of these gaseous and liquid effluents on the environment. It addresses the issue of radioactive wastes which are stored in the different nuclear base installations of the Centre, indicates the different measures aimed at limiting the volume of these warehoused wastes and addresses their impact on health and on the environment. Nature and quantities of warehoused wastes are specified. The second volume concerns some specific installations (INB 32 or ATPu, and INB 54 or LPC) which belong to AREVA NC. The same topics are addressed: presentation of the facilities, arrangements regarding safety and radiation protection, significant events related to safety and radiation protection, measurements of effluents and their impact on the environment, warehoused wastes. Remarks and recommendations of the CHSCT are given

  19. The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran

    International Nuclear Information System (INIS)

    Since several high level natural radiation areas (HLNRAs) exist on our planet, considerable attention has been drawn to health issues that may develop as the result of visiting or living in such places. City of Ramsar in Iran is an HNLRA, and is a tourist attraction mainly due to its hot spas. However, the growing awareness over its natural radiation sources has prompted widespread scientific investigation at national level. In this study, using an ELISA method, the level of expression of three tumor markers known as carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and carcino antigen 19-9 (CA19-9) in blood serum of 40 local men of Ramsar (subject group) was investigated and compared to 40 men from the city of Noshahr (control group). Noshahr was previously identified as a normal level natural radiation area (NLNRA) that is some 85 km far from Ramsar. According to statistical analysis, there was a significant difference in the levels of PSA and CA19-9 markers between the two groups (p < 0.001) with those of Ramsar being considerably higher. CEA level did not show any difference. Although some of the volunteers tested positive to the markers, they were in good health as confirmed by the physician. Moreover, the high number of positive markers in Noshahr was considerable. Therefore, future study is needed to further validate this result and to determine the level of positivity to tumor markers in both cities. -- Highlights: • Expression of three tumor markers was examined in 80 volunteers from Ramsar. • There was a significant difference in the levels of PSA and CA19-9 markers. • Some of the volunteers from the control city of Noshahr also tested positive. • Radiation might have caused an adaptive response in people of Ramsar. • Further study is necessary to re-confirm the positivity to tumor marker

  20. Numerical Platon: A unified linear equation solver interface by CEA for solving open foe scientific applications

    Energy Technology Data Exchange (ETDEWEB)

    Secher, Bernard [French Atomic Energy Commission (CEA), Nuclear Energy Division (DEN) (France); CEA Saclay DM2S/SFME/LGLS, Bat. 454, F-91191 Gif-sur-Yvette Cedex (France)], E-mail: bsecher@cea.fr; Belliard, Michel [French Atomic Energy Commission (CEA), Nuclear Energy Division (DEN) (France); CEA Cadarache DER/SSTH/LMDL, Bat. 238, F-13108 Saint-Paul-lez-Durance Cedex (France); Calvin, Christophe [French Atomic Energy Commission (CEA), Nuclear Energy Division (DEN) (France); CEA Saclay DM2S/SERMA/LLPR, Bat. 470, F-91191 Gif-sur-Yvette Cedex (France)

    2009-01-15

    This paper describes a tool called 'Numerical Platon' developed by the French Atomic Energy Commission (CEA). It provides a freely available (GNU LGPL license) interface for coupling scientific computing applications to various freeware linear solver libraries (essentially PETSc, SuperLU and HyPre), together with some proprietary CEA solvers, for high-performance computers that may be used in industrial software written in various programming languages. This tool was developed as part of considerable efforts by the CEA Nuclear Energy Division in the past years to promote massively parallel software and on-shelf parallel tools to help develop new generation simulation codes. After the presentation of the package architecture and the available algorithms, we show examples of how Numerical Platon is used in sequential and parallel CEA codes. Comparing with in-house solvers, the gain in terms of increases in computation capacities or in terms of parallel performances is notable, without considerable extra development cost.

  1. Comparison of Ablation Predictions for Carbonaceous Materials Using CEA and JANAF-Based Species Thermodynamics

    Science.gov (United States)

    Milos, Frank S.

    2011-01-01

    In most previous work at NASA Ames Research Center, ablation predictions for carbonaceous materials were obtained using a species thermodynamics database developed by Aerotherm Corporation. This database is derived mostly from the JANAF thermochemical tables. However, the CEA thermodynamics database, also used by NASA, is considered more up to date. In this work, the FIAT code was modified to use CEA-based curve fits for species thermodynamics, then analyses using both the JANAF and CEA thermodynamics were performed for carbon and carbon phenolic materials over a range of test conditions. The ablation predictions are comparable at lower heat fluxes where the dominant mechanism is carbon oxidation. However, the predictions begin to diverge in the sublimation regime, with the CEA model predicting lower recession. The disagreement is more significant for carbon phenolic than for carbon, and this difference is attributed to hydrocarbon species that may contribute to the ablation rate.

  2. Numerical Platon: A unified linear equation solver interface by CEA for solving open foe scientific applications

    International Nuclear Information System (INIS)

    This paper describes a tool called 'Numerical Platon' developed by the French Atomic Energy Commission (CEA). It provides a freely available (GNU LGPL license) interface for coupling scientific computing applications to various freeware linear solver libraries (essentially PETSc, SuperLU and HyPre), together with some proprietary CEA solvers, for high-performance computers that may be used in industrial software written in various programming languages. This tool was developed as part of considerable efforts by the CEA Nuclear Energy Division in the past years to promote massively parallel software and on-shelf parallel tools to help develop new generation simulation codes. After the presentation of the package architecture and the available algorithms, we show examples of how Numerical Platon is used in sequential and parallel CEA codes. Comparing with in-house solvers, the gain in terms of increases in computation capacities or in terms of parallel performances is notable, without considerable extra development cost

  3. The CEA and the alternative energies. Press tour 25 and 26 november 1999; Le CEA et les energies alternatives. Voyage de presse les 25 et 26 novembre 1999

    Energy Technology Data Exchange (ETDEWEB)

    Carola, G. [CEA/Grenoble, 38 (France); Ngo, Ch. [CEA, Dir. de la Strategie et de l' Evaluation, 75 - Paris (France); Mermilliod, N.; Serre-Combe, P. [CEA/Grenoble, Dir. des Technologies Avancees, DTA, 38 (France); Sanglan, P. [Air Liquide, 38 - Sassenage (France); De La Graviere, M. [CEA/Cadarache, 13 - Saint-Paul-lez-Durance (France); Dieudonne, O.; Malbranche, Ph. [CEA/Cadarache, Dir. des Reacteurs Nucleaires, DRN, 13 - Saint-Paul-lez-Durance (France)

    1999-11-01

    In the framework of the public information on the CEA center of Cadarache and Grenoble, a presentation of the researches concerning the alternative energies is proposed. The Cea is commissioned by the Public Authorities, to keep the nuclear option open and for the long-dated, to develop renewable energies. In this domain researches on fuel cells and photovoltaic solar energy are performed. The principle and the applications of the fuel cell and the photovoltaic are recalled to introduce the research programs and the partners. (A.L.B.)

  4. Design and cancer-targeting potential of antibody-based molecules directed against carcinoembryonic antigen.

    OpenAIRE

    Huhalov, A.

    2004-01-01

    This thesis examines the use of protein engineering to create antibody-based molecules for cancer treatment. The targeting unit used for these molecules was the single chain Fv antibody fragment MFE-23, which is directed against the tumour-associated marker carcinoembryonic antigen (CEA). It was hypothesised that implementation of molecular design features such as humanisation, high affinity, multivalency and mannose glycosylation to accelerate systemic clearance would result in the favourabl...

  5. Program improvements in the CEA service continuity: report and impact of the ice storm on CEA data

    Energy Technology Data Exchange (ETDEWEB)

    Billinton, R. [Saskatchewan Univ., Saskatoon, SK (Canada)

    1999-09-01

    The Canadian Electrical Association has been collecting statistical data on various aspects of electric power generation, transmission and distribution since 1979, when the Equipment Reliability Information System (ERIS) database has been implemented. Currently, the CEA publishes annual reports and statistics on generation and transmission component outages. Data gathering and reporting is monitored and guided by the Consultative Committee on Outage Statistics (CCOS). The Electric Power System Reliability Assessment (EPSRA) protocols represent the second stage of reliability data development. This system is designed to collect and report on data relating to the overall electricity supply system and the overall performance of the bulk electricity system. (ERIS reporting is concerned with equipment outages). This paper describes the Service Continuity Reporting System segment of the EPSRA protocols. Details of performance in service continuity for 1998 are provided with and without the 1998 ice storms effects. The complete data and indices on each participating utility, together with the overall Canada and total participating utility data, provides a convenient base for general utility comparisons. Comparisons of the various indices of reliability with like statistics from electric utility companies of Australia, New Zealand and the United States are also provided. 2 tabs., 1 fig.

  6. Scientific evaluation at the French Atomic Energy Commission (CEA). 2005-2006 annual report

    International Nuclear Information System (INIS)

    This report aims at presenting the scientific evaluation activities carried out at the CEA during the years 2005-2006. The evaluation system is described in detail. It comprises two aspects: the evaluation of the scientific policy implemented by the scientific Council and by the visiting committee of the CEA, and the evaluation of the laboratories, performed by a pool of 36 scientific councils. The evaluation by external and independent parties is the key point of this system. This document makes a status of the evaluations performed in 2005 and 2006. It presents a synthesis of the conclusions of the evaluation authorities. The actions implemented by the CEA to take into consideration the recommendations are also reported with the improvements noticed. The two topics examined by the scientific Committee and by the visiting committee were dealing with the energy domain, which is a strategic issue for the CEA. The examination of the researches on future nuclear reactors and on new energy technologies have shown the major role played by the CEA in the recent advances in these domains. About 95% of the laboratories activity was examined during the 2002-2005 period. The richness of the remarks and recommendations made by the scientific councils should allow the CEA to improve the quality and relevance of its research works. The start-up of the 2006-2009 evaluation cycle has been the occasion to modify the evaluation of some research domains in order to take into account the evolution of programs. The evaluation system of the CEA is highly consistent with the AERES principles. Its implementation, adapted to each type of activity (fundamental research, applied research, technological developments) allows the CEA to follow up a permanent improvement approach. (J.S.)

  7. Unusual elevation of CEA in a patient with history of colon cancer.

    Science.gov (United States)

    Hsu, Tzu-Chi

    2006-12-01

    A 35-year-old female received right hemicolectomy for a poorly differentiated adenocarcinoma of the ascending colon with lymph node metastasis (1/28) in February 1997. CEA was 1.68 ng/microl prior to colectomy. Adjuvant chemotherapy with weekly 5-FU and leucovorin intravenously was started following surgery and discontinued after 17 doses in May 1997. She received bilateral salpingo-ophorecctomy for metastatic cancer in August 1999. Intravenous chemotherapy was resumed with weekly 5-FU and leucovorin intravenously in August 1999. CEA was 93.8 ng/microl in November 1999. Intravenous chemotherapy was discontinued after 20 doses and oral chemotherapy with futraful and leucovorin was started in January 2000. CEA was found to be 240.3 ng/microl in December 1999 and then elevated to 1521.3 ng/microl in June 2001, which was 10 months after resection of metastatic ovarian cancer. No metastatic lesions could be detected, however, with image studies. The CEA decreased to 396.6 ng/microl three months later. Futraful was switched to uracil-tegafur (UFUR) in September 2001. The CEA for the patient ranged from 68.5 to 298.9 ng/microl for the following 5 years without aggressive chemotherapy. No evidence of recurrence could be demonstrated by imaging studies. The patient is not a smoker and denied exposure to a smoking environment. She was also not known to have persistent infections, inflammatory bowel disease, pancreatitis, cirrhosis of the liver, or any benign tumors. The current case suggested that: (i) elevation of CEA is not necessarily well correlated with presence of metastatic colon cancer; (ii) some patients may live with elevated CEA for years without evidence of recurrence or metastasis; (iii) aggressive chemotherapy may not be necessary in patients with only elevated CEA. PMID:17060406

  8. The Detection Value Analysis of Tumor Markers CEA,CA125 in Cervical Cancer%肿瘤标记物CEA、CA125在宫颈癌中的检测价值分析

    Institute of Scientific and Technical Information of China (English)

    戴伟萍

    2013-01-01

      目的:探讨肿瘤标记物 CEA、CA125在宫颈癌中的检测的临床价值.方法:选取笔者所在医院2011年1月-2012年8月经临床确诊的100例宫颈癌患者(宫颈癌组)及40例进行健康体检的妇女(对照组)为研究对象,测定两组的血清肿瘤标记物癌胚抗原(CEA)、糖抗原(CA125).结果:宫颈癌组的血清 CEA、CA125含量显著高于对照组(P<0.05).在宫颈癌组中,随着临床分期的增加 CEA、CA125的阳性率逐渐增加,Ⅲ、Ⅳ期较Ⅰ、Ⅱ期显著增加(P<0.05).结论:肿瘤标记物 CEA、CA125在宫颈癌中有较高的诊断价值,可辅助判断宫颈癌的临床分期.%Objective:To investigate the clinical value of tumor markers CEA,CA125 detection in cervical cancer and the significance.Methods:100 cases of cervical cancer (the cervical cancer group )that was clinical diagnosis from January 2011 to August 2012 in author’s hospital and 40 cases of healthy women (the control group) were chosen as the research object,two groups were measured in serum tumor marker carcinoembryonic antigen (CEA),carbohydrate antigens (CA125).Results:Serum CEA,CA125 in the cervical cancer group were significantly higher than those in the control group (P<0.05).In the cervical cancer group, CEA,CA125 positive rate increased gradually along with the clinical stagin,stage Ⅲ,Ⅳ were significantly than istage Ⅰ,Ⅱ (P<0.05).Conclusion:There is a high diagnostic value of the tumor markers CEA,CA125 in cervical carcinoma;they can help judge clinical cervical cancer staging.

  9. Relationship between serum carcinoembryonic antigen level and epidermal growth factor receptor mutations with the influence on the prognosis of non-small-cell lung cancer patients

    Directory of Open Access Journals (Sweden)

    Cai ZX

    2016-06-01

    Full Text Available Zuxun Cai Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou City, People’s Republic of China Objective: To investigate the relationship between serum carcinoembryonic antigen (CEA level and epidermal growth factor receptor (EGFR gene mutations in non-small-cell lung cancer (NSCLC patients and to analyze the influence of CEA level on postoperative survival time in lung cancer patients. Methods: A total of 296 patients who were treated in Thoracic Surgery Department of Henan Provincial Chest Hospital from September 2011 to September 2013 were recruited. The level of tumor markers, such as CEA, was determined before the surgery, and EGFR gene mutations were detected after surgery. Thereby, the relationship between tumor makers, including CEA, and EGFR mutation and its influence on prognosis could be investigated. Results: Among 296 patients, the positive rate of EGFR gene mutation was 37.84% (112/296; the mutation occurred more frequently in nonsmokers, adenocarcinoma patients, women, and patients aged <60 years (P<0.05. Both tumor markers and chemosensitivity indicators were related to the profile of EGFR mutations. Elevated squamous cell carcinoma and Cyfra21-1 as well as positively expressed ERCC1 were more common in patients with wild-type EGFR (P<0.05, whereas increased CEA level was observed more frequently in patients with EGFR gene mutation (P=0.012. The positive rate of EGFR gene mutations was higher as the serum CEA level increased, that is, the positive rate in patients with serum CEA level <5, 5–20, and >20 µg/L was 39.81%, 45.32%, and 65.47%, respectively (P=0.004. Logistic regression analysis showed that CEA level was an independent factor in predicting EGFR gene mutations, and serum CEA level was also an independent factor in affecting the prognosis of NSCLC patients, as the overall 2-year survival rate was 73.86% in elevated CEA group and 86.43% in normal group (P<0.01. Conclusion: The prognosis of

  10. Situation 2002: release monitoring and surveillance of environment of Cea centers; Bilan 2002: controle des rejets et surveillance de l'environnement des centres CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This publication renders an account of the situation of the releases of liquid and gaseous radioactive effluents, for the year 2002, as well as the radioactivity levels measured in the vicinity of Cea centers through the systematic surveillance of atmosphere, waters, vegetation and milk. An analysis on five years allows to follow their evolution. (N.C.)

  11. 血清 TSA 和 CEA 联合检测对2型糖尿病合并结直肠癌的诊断价值%Diagnostic value of combined detection of serum TSA and CEA in type 2 diabetes mellitus complicating colorectal cancer

    Institute of Scientific and Technical Information of China (English)

    刘瑞东; 王小丽

    2016-01-01

    目的:探讨血清总唾液酸(TSA)和癌胚抗原(CEA)联合检测对2糖尿病合并结直肠癌患者的诊断价值。方法选取结直肠癌合并2型糖尿病患者60例(A组),单纯结直肠癌患者55例(B组),同期的健康者60例(C组),采用化学发光法检测各组血清TSA和CEA水平;对手术切除治疗的患者进行随访半年,检测血清TSA和CEA水平。结果与B组(34.55%)和C组(1.67%)相比较,A组 TSA和CEA联合检测敏感率最高(78.33%),差异具有统计学意义(P<0.05);且A组患者血清TSA[(791.26±135.90)mg/L]及CEA[(48.45±2.67)ng/mL]水平高于B组和C组,差异有统计学意义(P<0.01);A组和B组血清 TSA和CEA水平均较手术前降低,差异有统计学意义(P<0.05)。结论联合检测血清TSA和CEA水平,能为2型糖尿病合并结直肠癌的诊断、预后、复发提供更早、更灵敏的检测指标。%Objective To investigate the diagnostic value of combined detection of serum total sialic acid (TSA) and carcino‐embryonic antigen (CEA) in type 2 diabetes mellitus (T2DM ) complicating colorectal cancer . Methods Sixty of T2DM complicating colorectal cancer were selected as the group A ,other 55 cases of pure colorec‐tal cancer were selected as the group B and contemporaneous 60 healthy persons as the group C .The levels of serum TSA and CEA in each group were detected by chemiluminescence method;at the same time ,the factors of ages ,body mass index (BMI) and series of tumor biochemical detection were compared among various groups;the cases of sur‐gical resection were followed up for 6 months ,and serum TSA and CEA levels were detected .Results Compared with group B (34 .55% ) and C (1 .67% ) ,the sensitivity of combined detection of TSA and CEA was highest (78 .33% ) ,and the difference had statistical significance (P<0 .05);moreover the levels of serum CEA and TSA in the group A

  12. Histocompatibility antigen test

    Science.gov (United States)

    ... more common in certain autoimmune diseases . For example, HLA-B27 antigen is found in many people (but not ... More Ankylosing spondylitis Autoimmune disorders Bone marrow transplant HLA-B27 antigen Kidney transplant Reactive arthritis Update Date 2/ ...

  13. Biodistribution of anti-CEA F(ab')[sub 2] fragments conjugated with chelating polymers: influence of conjugate electron charge on tumor uptake and blood clearance

    Energy Technology Data Exchange (ETDEWEB)

    Slinkin, M.A.; Curtet, C.; Faivre-Chauvet, A.; Sai-Maurel, C.; Gestin, J.F.; Chatal, J.F. (INSERM U.211, Nantes (France)); Torchilin, V.P. (Massachusetts General Hospital, Boston, MA (United States))

    1993-05-01

    F(ab')[sub 2] fragments of anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb) were modified with three chain-terminal polysine-based chelating polymers so as to carry different electron charges. Immunoreactive conjugates labeled with [sup 111]In up to a specific radioactivity of 120-140 [mu]Ci/[mu]g were injected into nude mice bearing humans colorectal carcinoma, and the biodistribution patterns were compared with each other and with that of an anti-CEA F(ab')[sub 2]-DTPA control. Immunoconjugate modified with positively-charged polymer produced the highest tumor uptake [up to 20% injected dose per gram (ID/g)], with very significant non-specific radioactivity in normal organs (particularly kidneys). When modified with a polymer carrying only a slight negative charge, the immunoconjugate also produced fairly high tumor uptake (up to 18% ID/g), with much lower non-specific radioactivity in normal organs. Highly negatively-charged conjugate produced the lowest tumor uptake (up to 8% ID/g), whereas blood and whole-body clearances were the fastest but slower than those of conventionally labeled F(ab')[sub 2] mAb. The possible mechanisms for the effects described are discussed. (author).

  14. Fitoplancton del Parque Nacional Las Tablas de Daimiel. II. Las cianofíceas, los dinoflagelados, las criptofíceas, las crisofíceas y las xantofíceas

    Directory of Open Access Journals (Sweden)

    Rojo, Carmen

    1999-12-01

    Full Text Available Las Tablas de Daimiel National Park is a semiarid wetland that exhibits a high biodiversity but is suffering an inexorable eutrophication process. The study of the phytoplankton in this wetland is interesting because of its hydrological fluctuations (drought period until 1996 and its trophic condition. This papers shows the taxonomy, dynamic, and ecology of microalgae in five áreas of the Park from 1996 to 1998, which allow comparisons to be made between the present state and the 1992-1993 period. Twelve taxa of Cyanophyta, 4 of Dynophyceae, 8 of Cryptophyceae, 5 of Chrysophyceae and one of Xanthophyceae were found. Almost all species have been already cited in Spain and are typical for eutrophic environments. Cyanophyceae and Cryptophyceae were the most important groups (Planktothrix agardhii, Anabaenopsis elenkinii, Cryptomonas erosa as the most common algae. Moreover, oscillatorial Cyanophyceae (in 1992 have been replaced by N-fixing nostocal ones, related to the high level of pollution found in recent years.El Parque Nacional Las Tablas de Daimiel es un humedal semiárido, importante reserva de biodiversidad que, sin embargo, está sufriendo un inexorable proceso de eutrofización. Su estado trófico, así como los cambios hidrológicos (sequía hasta 1996, hacen especialmente interesante el estudio de su microflora. Por ello, se ha llevado a cabo este trabajo sobre las poblaciones fitoplanctónicas en cinco puntos del Parque desde 1996 a 1998, que permite además la comparación con su estado en 1992-1993. Se encontraron 12 táxones de Cyanophyta, 4 de Dynophyceae, 8 de Cryptophyceae, 5 de Chrysophyceae y uno de Xanthophyceae. Casi todas las especies están citadas en España y son propias de ambientes eutróficos. Los grupos mejor representados fueron las cianobacterias y criptofíceas (Planktothrix agardhii, Anabaenopsis elenkinii y Cryptomonas erosa fueron las especies más conspicuas. Además, se observa un cambio de cianobacterias

  15. Diagnostic sensitivity of serum carcinoembryonic antigen, carbohydrate antigen 19-9, alpha-fetoprotein, and beta-human chorionic gonadotropin in esophageal carcinoma (receiver operating characteristic curve analysis

    Directory of Open Access Journals (Sweden)

    Bhawna Bagaria

    2015-01-01

    Full Text Available Background: Esophageal carcinomas are very lethal disease relatively unresponsive to therapy. The continued development of new and more effective chemotherapeutic agents and regimens offers hope that in the future, this carcinoma may be amenable to either more effective palliative treatment or possibly increased cure. We, therefore, aimed to evaluate the marker with best diagnostic sensitivity in esophageal carcinoma. Materials and Methods: Serum carcinoembryonic antigen (CEA, carbohydrate antigen 19-9 (CA19-9, alpha-fetoprotein (AFP, and beta-human chorionic gonadotropin (β-HCG levels were assessed in healthy subjects (n = 50 and patients (n = 50 initially diagnosed of esophageal carcinoma by endoscopic examination and biopsy before receiving any therapy. The data were analyzed using SPSS software version 10.0 (SPSS Inc. USA and MedCalc to estimate mean ± standard deviation, the significance of the observed differences (P value, for calculating sensitivity and for plotting receiver operating characteristic curves. Results: Sensitivity of CEA, CA19-9, AFP, and β-HCG detected in esophagus cancer was 38%, 18%, 10%, and 26% respectively. Conclusion: From the above studied markers, CEA has the highest sensitivity followed by β-HCG, CA19-9 and AFP. Although the sensitivity of tumor markers in esophagus cancer is low, they may be useful additional parameter in the prediction of neoplasms involved at the early stage of tumor growth.

  16. Evaluation of the reliability concerning the identification of human factors as contributing factors by a computer supported event analysis (CEA)

    International Nuclear Information System (INIS)

    The project's objectives are the evaluation of the reliability concerning the identification of Human Factors as contributing factors by a computer supported event analysis (CEA). CEA is a computer version of SOL (Safety through Organizational Learning). Parts of the first step were interviews with experts from the nuclear power industry and the evaluation of existing computer supported event analysis methods. This information was combined to a requirement profile for the CEA software. The next step contained the implementation of the software in an iterative process of evaluation. The completion of this project was the testing of the CEA software. As a result the testing demonstrated that it is possible to identify contributing factors with CEA validly. In addition, CEA received a very positive feedback from the experts. (orig.)

  17. Clinical significance of joint detection of serum CEA, SCCA, and bFGF in the diagnosis of lung cancer

    OpenAIRE

    Zhao, Wei; Yu, Haixiang; Han, Zhifeng; Gao, Nan; Xue, Jinru; Wang, Yan

    2015-01-01

    Lung cancer is a type of malignant tumor with highest morbidity and mortality. This study tested three tumor marker levels including CEA, SCCA, and bFGF to explore their value in lung cancer diagnosis and pathological type judgment. Venous blood was extracted from lung cancer patients, lung benign lesion patients and healthy control. Electrochemiluminescence immunoassay was applied to detect serum CEA and SCCA content. ELISA was used to test serum bFGF level. Serum CEA, SCCA, and bFGF levels ...

  18. D-Dimer and Carcinoembryonic Antigen Levels: Useful Indicators for Predicting the Tumor Stage and Postoperative Survival.

    Science.gov (United States)

    Tekeşin, Kemal; Bayrak, Savaş; Esatoğlu, Varol; Özdemir, Ebru; Özel, Leyla; Melih Kara, Veli

    2016-01-01

    The purpose of this prospective study is to determine the preoperative plasma D-dimer and serum Carcinoembryonic Antigen (CEA) levels of patients scheduled for curative surgical resection for colorectal cancer and to evaluate the significance of these levels on the prognosis and postoperative survival rate. One hundred sixty-five patients with colorectal cancer, who were scheduled to have elective resection between January 2008 and January 2011, were included in the study. A significant increase was observed in the D-dimer levels, particularly in poorly differentiated tumors. The distance covered by the tumor inside the walls of the colon and rectum (T-stage) was significant for both D-dimer and CEA levels. As the T-stage increased, there was also a significant increase in the D-dimer and CEA levels. A high significance and correlation level was detected between the TNM staging and both D-dimer and CEA. A significant relationship was found between the advanced tumor stage and short postoperative survival rate of patients with colorectal cancer. Therefore, the analysis of preoperative D-dimer and CEA levels can be useful in predicting the stage and differentiation of the tumor and the postoperative survival rate. PMID:27651789

  19. How to Add Value to your Business with CEA: A Practical Approach

    Directory of Open Access Journals (Sweden)

    Daniel Cardenas

    2010-04-01

    Full Text Available Companies are always trying to differentiate themselves from the rest of the pack by applying different strategies such as improving customer service, increasing the efficiency of their operations, or reducing their costs. Most of the time, however, these goals are competing against each other for scarce resources, and managers often need to decide to concentrate on one. A small company can effectively and simultaneously accomplish these goals for a fraction of the cost by implementing communications-enabled business processes or solutions, which are a set of technology components that add real-time networking functionality to applications. One particular implementation of this framework is the one provided by Coral CEA. Coral CEA is a business ecosystem anchored around CEA functionalities that are offered as building blocks, out-of-the-box components that link the capabilities and intelligence of networks platforms with the power of current applications to provide a new set of features and functionalities. In this article, we show how a small company called Rezact, located in the ski resort town of Mont-Tremblant, Quebec, successfully implemented CEA capabilities within its own operations using Coral CEA services.

  20. Construction, Expression and Characterization of a Chimeric Protein Targeting Carcinoembryonic Antigen in Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    LI Yang; HUA Shu-cheng; MA Cheng-yuan; YU Zhen-xiang; XU Li-jun; LI Dan; SUN Li-li; LI Xiao; PENG Li-ping

    2011-01-01

    The carcinoembryonic antigen(CEA) is an oncofetal glycoprotein known as an important clinical tumor marker and is overexpressed in several types of tumors, including colorectal and lung carcinomas. We constructed a chimeric protein that exhibits both specific binding and immune stimulating activities, by fusing staphylococcal enterotoxin A(SEA) to the C-terminus of an anti-CEA single-chain disulfide-stabilized Fv(scdsFv) antibody (single-chain-C-terminus/SEA, SC-C/SEA). The SC-C/SEA protein was expressed in Escherichia coli(E. coli), refolded, and purified on an immobilized Ni2+ affinity chromatography column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot analysis reveal that the target protein was expressed sufficiently. We used immunofluorescence assays to demonstrate that SC-C/SEA could bind specifically to human lung carcinoma cells(A549), but almost human uterine cervix cells(HeLa). We also used the L-lactate dehydrogenase(LDH) release assay to show that SC-C/SEA elicits a strong A549 tumor-specific cytotoxic T lymphocyte(CTL) response in vitro. The results suggest that SC-C/SEA shows specific activity against CEA-positive cells and has potential application in CEA-targeted cancer immunotherapy.

  1. A Sandwich Electrochemical Immunosensor Using Magnetic DNA Nanoprobes for Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Ning Gan

    2011-10-01

    Full Text Available A novel magnetic nanoparticle-based electrochemical immunoassay of carcinoembryonic antigen (CEA was designed as a model using CEA antibody-functionalized magnetic beads [DNA/Fe3O4/ZrO2; Fe3O4 (core/ZrO2 (shell nano particles (ZMPs] as immunosensing probes. To design the immunoassay, the CEA antibody and O-phenylenediamine (OPD were initially immobilized on a chitosan/nano gold composite membrane on a glassy carbon electrode (GCE/CS-nano Au, which was used for CEA recognition. Then, horseradish peroxidase (HRP-labeled anti-CEA antibodies (HRP-CEA Ab2 were bound to the surface of the synthesized magnetic ZMP nanoparticles as signal tag. Thus, the sandwich-type immune complex could be formed between secondary antibody (Ab2 modified DNA/ZMPs nanochains tagged by HRP and GCE/CS-nano Au. Unlike conventional nanoparticle-based electrochemical immunoassays, the recognition elements of this immunoassay included both electron mediators and enzyme labels, which obviously simplifies the electrochemical measurement process. The sandwich-type immunoassay format was used for online formation of the immunocomplex of CEA captured in the detection cell with an external magnet. The electrochemical signals derived from HRP during the reduction of H2O2 with OPD as electron mediator were measured. The method displayed a high sensitivity for CEA detection in the range of 0.008–200 ng/mL, with a detection limit of 5 pg/mL (estimated at a signal-to-noise ratio of 3. The precision, reproducibility, and stability of the immunoassay were good. The use of the assay was evaluated with clinical serum samples, and the results were in excellent accordance with those obtained using the standard enzyme-linked immunosorbent assay (ELISA method. Thus, the magnetic nanoparticle-based assay format is a promising approach for clinical applications, and it could be further developed for the detection of other biomarkers in cancer diagnosis.

  2. Higher importance of interleukin 6 than classic tumor markers (carcinoembryonic antigen and squamous cell cancer antigen) in the diagnosis of esophageal cancer patients.

    Science.gov (United States)

    Łukaszewicz-Zając, M; Mroczko, B; Kozłowski, M; Nikliński, J; Laudański, J; Szmitkowski, M

    2012-04-01

    It has been suggested that interleukin 6 (IL-6) plays a potential role in the growth and progression of tumors, including esophageal cancer (EC). The aim of the study was to compare clinical significance of serum IL-6 with classic tumor markers - carcinoembryonic antigen (CEA) and squamous cell cancer antigen (SCC-Ag) - in EC patients in relation to its histological types - squamous cell carcinoma of esophagus (ESCC) and adenocarcinoma (AD) of esophagus. The study included 53 EC patients and 90 healthy subjects. Serum IL-6 and CEA levels were determined using immunoenzyme assays, while SCC-Ag - chemiluminescent assay. The diagnostic criteria and prognostic values for markers were defined. The levels of all proteins tested in EC, ESCC, and AD were higher than in healthy subjects. The percentage of elevated results was substantially higher for IL-6 (86%) than for CEA (30%) and SCC-Ag (24%) in EC, similarly as in ESCC (87%, 23%, and 33%) and AD (87%, 39%, and 13%, respectively) patients. Concentrations of IL-6 depended on distant metastases and patients' survival in EC and were significantly higher in ESCC patients with more advanced tumor stage and nodal metastases. The IL-6 area under receiver operating characteristic curve (0.92) was larger than for CEA (0.84) and SCC-Ag (0.62) in EC, likewise in ESCC (0.92, 0.87, 0.77) and AD (0.91, 0.79, 0.57, respectively). Our findings indicate better usefulness of IL-6 than classic tumor markers in the diagnosis of EC, especially in patients with ESCC.

  3. Overview of CEA research in the field of radionuclides migration; Syntheses des recherches menees par le CEA sur la migration des radionucleides

    Energy Technology Data Exchange (ETDEWEB)

    Poinssot, Ch.; Trotignon, L.; Tevissen, E

    2006-07-01

    This report presents a synthetic status of the researches conducted within the Nuclear Energy Division (CEA/DEN) in the field of radionuclides migration in three specific areas which have been chosen for their representativeness and potential impact: the migration of RN in PWR reactors, the migration of RN from a deep geological repository and the migration processes in the surface environments. In addition, some status is given about more generic research which is conducted in the field of RN speciation in the aqueous phase and at the interfaces and regarding chemistry / transport couplings. Additional information about the human and technical means involved in these fields of research in CEA/DEN is finally given in the Appendix. (authors)

  4. Combined detection of CEA, CA19-9 and CA125 in the differential diagnosis of benign and malignant ascites%联合检测CEA、CA19-9和CA125对鉴别良恶性腹水的价值

    Institute of Scientific and Technical Information of China (English)

    卢冰贤; 周云英

    2011-01-01

    目的 探讨检测CEA(癌胚抗原)、CA19-9(糖类抗原19-9)和CA125(糖类抗原125)对鉴别良、恶性腹水的临床价值. 方法 化学发光法检测120例患者腹水CEA、CA19-9和CA125的含量. 结果 化学发光法检测恶性腹水中的CEA、CA19-9和CA125分别为(37±16) ng/ml、(236±78) U/ml和(602士211)U/ml,良性腹水分别为(13±2)ng/ml、(34士3)U/ml和(32±3)U/ml,差异均有统计学意义(P<0.05).同时,3种抗原检测的敏感性和特异性各有特点,需要联合检测. 结论 联合检测良、恶性腹水中CEA、CA19-9和CA125水平对鉴别诊断有一定临床意义.%Objective To explore the clinical value of detecting CEA (cancer embryo antigen), CA19-9 (sugar 19-9 antigen), and CA125 (sugar antigen 125) to differentiate benign and malignant ascites. Methods CA19-9, CEA, and CA125 levels were determined by chemiluminescence detection in 120 patients with ascites. Results Chemiluminescence of patients with malignant ascites revealed a CEA level of (37± 16) ng/ml, a CA19-9 level of (236±78) U/ml, and a CA125 level of (602 + 211) U/ml while chemiluminescence of patients with benign ascites revealed a CEA level of (13± 2) ng/ml, a CA19-9 level of (34±3) U/ml and a CA125 level of (32 ± 3) U/ml. Differences were statistically significant (P<0. 05). Testing with 3 different antigens revealed a particular sensitivity and specificity, and combined testing was required. Conclusion Combined testing of CA19-9, CEA, and CA125 levels has some clinical significance in the differential diagnosis of benign and malignant ascites.

  5. Report of transparency and nuclear safety 2007 CEA Fontenay aux Roses; Rapport transparence et securite nucleaire 2007 CEA Fontenay aux Roses

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the activities of the CEA Center of Fontenay aux roses for the year 2007. After many years of decommissioning and dismantling of nuclear installations, the Center is now devoted (since 2005) to the development of research programmes on biology and biomedical technologies. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. (A.L.B.)

  6. Latest pixel size reduction of uncooled IR-FPA at CEA, LETI

    Science.gov (United States)

    Becker, Sebastien; Imperinetti, Pierre; Yon, Jean-Jacques; Ouvrier-Buffet, Jean-Louis; Goudon, Valérie; Hamelin, Antoine; Vialle, Claire; Arnaud, Agnès.

    2012-10-01

    Recent developments at the Infrared Lab (LIR) of CEA, LETI have been concentrated on the pixel size reduction of uncooled infrared detectors. With the support from French company ULIS, we have successfully demonstrated the technological integration of 12μm pixels on a commercial TV-format read-out circuit (VGA-ROIC) supplied by ULIS. The 12μm pixel has been designed, processed and characterized in CEA, LETI and first results showed exceptional performances. This paper presents the characterization and associated imagery results.

  7. Aeroacoustics research in Europe: The CEAS-ASC report on 2003 highlights

    Science.gov (United States)

    Schröder, W.

    2004-11-01

    This is a report on some highlights of aeroacoustics research in Europe in 2003, compiled from information provided to the Aeroacoustics Specialists Committee of the Confederation of European Aerospace Societies (CEAS). The CEAS currently comprises the national Aerospace Societies of France (Association Aéronautique et Astronautique de France), Germany (Deutsche Gesellschaft für Luft- und Raumfahrt), Italy (Associazione Italiana di Aeronautica e Astronautica), The Netherlands (Nederlandse Vereniging voor Luchtvaarttechniek), Spain (Asociación de Ingenieros Aeronáuticos de España), Sweden (Flygtekniska Föreningen), Switzerland (Schweizerische Vereinigung für Flugwissenschaften) and the UK (The Royal Aeronautical Society).

  8. Changes in the legal status of the Commissariat a l'energie atomique (Cea)

    International Nuclear Information System (INIS)

    The Cea a public research institution was re-classified among the energy research establishments (11 december 2008). The Cea, as the result of exceptional circumstances and complex activities, is today entering into a new era and must confront many national and supranational challenges. It is preparing, in effect, for a future nuclear industry (safer, less polluting, non-proliferating and more economical) and is conducting research into other energy solutions within the framework of sustainable development and limited greenhouse gas effects (hydrogen technology, fusion, alternative energy technologies). It also has another goal, to make technological research available to industry, not only by developing micro and nano-technologies or even to contribute technology for the benefit of scientific knowledge of living creatures, matter, climate and the environment. The new classification of the Cea as a research establishment in the field of energy, a heading which includes three other entities (A.N.D.R.A., A.D.E.M.E. and I.F.P.), raises questions about its specialization and diversification. However, no substantive change was made to the drafting of the legislative provisions so it is possible to imagine that the Cea takes initiatives in other fields, at the instigation or with the approval of the government, a matter which is facilitated by its classification in the E.P.I.C. category (public establishment at industrial and commercial character). The Cea also represents France in international organisations in the nuclear sector, such as the OECD Nuclear energy agency (Nea), the International atomic energy agency (IAEA) and the European communities. It also participates in intergovernmental negotiations in the nuclear field and ensures any follow-up required with regard to any resulting agreements. today, the Cea plays a major role in the implementation of French nuclear policy with the aim of making available to countries wishing to develop nuclear energy, a framework

  9. The role of CA-242 and CEA in surveillance following curative resection for colorectal cancer.

    OpenAIRE

    Hall, N. R.; Finan, P. J.; Stephenson, B M; Purves, D. A.; Cooper, E H

    1994-01-01

    This study was undertaken to evaluate the role of a new tumour marker, CA-242, alone or in combination with CEA in the practical management of colorectal cancer patients after potentially curative resection. A cohort of 149 patients who had undergone 'curative' surgery was followed up according to an intensive protocol in order to detect recurrent disease. Over a median tumour marker follow-up period of 24 months there were 25 recurrences in 24 patients. Both CEA and CA-242 alone detected hal...

  10. Performances of Herschel/PACS bolometer arrays and future developments at CEA

    OpenAIRE

    Billot, N.; RODRIGUEZ, L.; Okumura, K; Sauvage, M.; Agnèse, P.

    2009-01-01

    The European Space Agency is building a space telescope to observe the Universe in the Far-IR and sub-millimeter regime of the electromagnetic spectrum. The scientific payload is composed of three instruments. One of them, PACS, is equipped with a novel type of bolometer arrays developed by CEA in the late 90's. We briefly present the PACS Photometer and the architecture of CEA filled bolometer arrays. We accessed the physics of the detectors and thoroughly measured their performances by deve...

  11. The CEA and the alternative energies. Press tour 25 and 26 november 1999

    International Nuclear Information System (INIS)

    In the framework of the public information on the CEA center of Cadarache and Grenoble, a presentation of the researches concerning the alternative energies is proposed. The Cea is commissioned by the Public Authorities, to keep the nuclear option open and for the long-dated, to develop renewable energies. In this domain researches on fuel cells and photovoltaic solar energy are performed. The principle and the applications of the fuel cell and the photovoltaic are recalled to introduce the research programs and the partners. (A.L.B.)

  12. Differential diagnosis of tuberculosis and lung cancer pleural effusion by CEA, CA125,CA50%CEA、CA125、CA50在结核及肺癌性胸水鉴别中的意义

    Institute of Scientific and Technical Information of China (English)

    冯金栋; 赵卫国; 保鹏涛

    2013-01-01

    Objective:To explore the significance of differential diagnosis of pleural effusion by detection of carci-noembryonic antigen (CEA) ,carbohydrate antigen (CA125) and carbohydrate antigen (CA50). Methods: A total of 126 specimens from pleural effusion diagnosed as TB in 70 cases and lung cancer in 56 cases were collected. CEA, CA125,CA50 in the pleural effusion were analyzed. ResuItS:The expression level and sensitivity of CEA and CA50 in pleural effusion of lung cancer was significantly higher than that in tuberculous (P 0.05). The specificity was only 5.71%. Combining CEA with CA125 to diagnose pleural effusion resulted fom lung cancer, the sensitivity was 92. 11% and combining CA125 with CA50 the sensitivity was 95. 24% . When the concetration of CEA was more than 10ng/ml or the concetration of CA125 was more than 2000U/ml,pleural effusion of lung cancer should be highly suspected. Conclusion;The use of CEA.CA125 and CA50 separately,or in combination is helpful to differential diagnosis of pleural effusion resulted from tuberculosis and lung cancer.%目的:探讨癌胚抗原(CEA)、糖类抗原(CA125)、糖类抗原(CA50)在胸水鉴别诊断中的意义.方法:收集已明确胸水性质的标本126例(结核性70例、癌性56例),检测胸水中CEA、CA125、CA50的数值,进行统计分析.结果:肺癌性胸水中CEA、CA50表达水平及敏感度明显高于结核性胸腔积液(P<0.05),特异性分别为95.71%和98.57%.同样CA125在肺癌性胸水中高表达(P<0.05),但两组敏感度无差异(P>0.05),特异性仅为5.71%;CEA和CA125联合诊断肺癌性胸水的敏感度为92.11%,CA125和CA50联合诊断敏感度为95.24%.CEA> 10ng/ml或CA125> 2000U/ml,可初步定为肺癌性胸水.结论:CEA、CA125、CA50单独或联合检测对鉴别诊断结核及肺癌性胸水有重要临床指导意义.

  13. 血清CA125、CEA水平与宫颈癌相关性分析%Association of serum carcinoembryonic antigen and carbohydrate antigen 125 with cervical cancer

    Institute of Scientific and Technical Information of China (English)

    胡跃华

    2012-01-01

    Objective To analyze the association of serum carcinoembryonic antigen ( CEA )and carbohydrate antigen 125 ( CA125 ) with cervical cancer.Methods 100 patients with cervical cancer who had been treated in our hospital during the period of February 2007 to March 2011 were enrolled into a study group.Serum levels of CEA and CA125 were detected and their assoication with cervical cancer was analyzed; and the levels of these markers were compared with those from 60 non-cervical cancer patients ( control group ).Results Serum levels of CEA and CA125 were higher in the study group than in the control group,with a significant difference between 2 groups ( P< 0.05 ) except for stage Ⅰ cervical cancer.The correlation between CA125 and stages of cervical cancer was greater than that between CEA and cervical cancer ( P< 0.05 ).Conclusions Serum levels of CEA and CA125 are associated with cervical cancer at some degree.CA125 has more diagnostic values than CEA.%目的 分析研究血清CA125(糖链抗原125)、CEA(癌胚抗原)水平与宫颈癌的相关性.方法 将我院从2007年2月至2011年3月收治的100例宫颈癌病患设为实验组,进行血清CA125、CEA水平检测,并与宫颈癌分期相分析,将同期收治的60例非宫颈癌病患设为对照组,对比两组血清CA125、CEA水平.结果 实验组血清CA125、CEA水平均高于对照组,除Ⅰ期宫颈癌数据外,差异有统计学意义(P<0.05);血清CA125与宫颈癌分期的相关性高于血清CEA,差异有统计学意义(P<0.05 ).结论 血清CA125、CEA水平与宫颈癌有一定相关性,检测简单方便,且血清CA125比血清CEA更具诊断价值.

  14. Assessment 2000 and regulation and method. Releases control and environmental survey of the CEA Centers; Bilan 2000 et reglementation et methode. Controle des rejets et surveillance de l'environnement des centres CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    The environment quality around the CEA centers is a major interest of its safety policy. These documents contribute to the public information on the radioactive liquid and gaseous releases of the CEA, according to the ministry authorization. The radioactivity monitoring activity, and the survey methods are also presented. Data analysis from 1996 to 2000, allows to follow the evolution. (A.L.B.)

  15. Expression and clinic significance of HSP gp96 and CEA in colorectal carcinoma%HSP gp96和CEA在结直肠癌中的表达及临床意义的研究

    Institute of Scientific and Technical Information of China (English)

    杨松海; 邓跃华; 刘传磊; 李帅; 刘弋

    2012-01-01

    目的 通过检测结直肠癌组织及相应癌旁组织中的热休克蛋白糖蛋白96(Heat Shock Protein glycoprotein 96,HSP gp96)和癌胚抗原(Carcino Embryonic Antigen,CEA)的表达,探讨HSP gp96和CEA在结直肠癌组织中的表达及临床意义.方法 应用免疫组织化学方法检测88例结直肠癌及相应癌旁组织中的HSP gp96和CEA的表达情况.结果 HSP gp96在结直肠癌组织中的表达率明显高于癌旁组织(χ2=47.400,P0.05);CEA在结直肠癌组织中的表达率明显高于癌旁组织(χ2=52.391,P0.05);且 HSP gp96和CEA的表达存在关联(χ2=9.374,P 0.05 ). The expression of CEA in colorectal cancer tissues was significantly higher than that in the adjacent tissues( x2 =52. 391 ,P 0.05 ). Moreover,there was positive correlation between the expression of HSP gp96 and CEA( x2 =9. 374,P <0.05,r = 0. 326 ). Conclusion HSP gp96 and CEA participate in development and metastasis of colorectal cancer; HSP gp96 may be a new tumor marker of colorectal cancer.

  16. Mesothelioma: profile of keratin proteins and carcinoembryonic antigen: an immunoperoxidase study of 20 cases and comparison with pulmonary adenocarcinomas.

    OpenAIRE

    Corson, J M; Pinkus, G. S.

    1982-01-01

    The distribution of keratin proteins and carcinoembryonic antigen (CEA) in 20 diffuse pleural malignant mesotheliomas and 20 adenocarcinomas of the lung was determined with the use of an indirect immunoperoxidase method. Keratin proteins were identified in all of the mesotheliomas, with strong staining observed in 17 of the cases. Tumor cells of various histologic types (tubular, papillary, solid, and spindle) revealed staining for keratin proteins. A variety of staining patterns were observe...

  17. Sensitivity of CA 15-3, CEA and serum HER2 in the early detection of recurrence of breast cancer

    DEFF Research Database (Denmark)

    Pedersen, Ann Christina; Sørensen, Patricia Diana; Jacobsen, Erik Hugger;

    2013-01-01

    The aim of this project was to investigate the sensitivity of CA 15-3, CEA and HER2 in the early diagnosis of metastatic breast cancer.......The aim of this project was to investigate the sensitivity of CA 15-3, CEA and HER2 in the early diagnosis of metastatic breast cancer....

  18. Clinical value of combined detection of serum CEA, CA15-3 and SF in lung cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical value of combined examination of serum CEA, CA15-3 and SF in lung cancer diagnosis. Methods: Serum concentrations of CEA, CA15-3 and SF were measured by chemiluminescent assay (CLA) in 99 patients with lung cancer and 25 patients with benign lung disease. Results: The positive rate of serum CEA, CA15-3 and SF in cases in lung cancer was 50.5%, 31.3% and 69.7%, respectively. Specificity of CEA, CA15-3 and SF measurements was 76%, 78% and 72%, respectively. In combined measurements, positive results of either two parameters revealed sensitivity and specificity of 82.8% and 92%, respectively. Conclusion: Combined measurements of CEA, CA15-3 and SF had evidently improved sensitivity and specificity in diagnosing lung cancer and thus were of good clinical value

  19. A study on the core analysis methodology for SMART CEA ejection accident-I

    Energy Technology Data Exchange (ETDEWEB)

    Zee, Sung Kyun; Lee, Chung Chan; Kim, Kyo Yoon; Cho, Byung Oh

    1999-04-01

    A methodology to analyze the fuel enthalpy is developed based on MASTER that is a time dependent 3 dimensional core analysis code. Using the proposed methodology, SMART CEA ejection accident is analyzed. Moreover, radiation doses are estimated at the exclusion area boundary and low population zone to confirm the criteria for the accident. (Author). 31 refs., 13 tabs., 18 figs.

  20. A study on the core analysis methodology for SMART CEA ejection accident-I

    International Nuclear Information System (INIS)

    A methodology to analyze the fuel enthalpy is developed based on MASTER that is a time dependent 3 dimensional core analysis code. Using the proposed methodology, SMART CEA ejection accident is analyzed. Moreover, radiation doses are estimated at the exclusion area boundary and low population zone to confirm the criteria for the accident. (Author). 31 refs., 13 tabs., 18 figs

  1. Meeting of Directors and Heads of Department from CEA-Saclay, France

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    Left to right: Ph. Pradelles, M. Jacquemet and T. Taylor in discussion during the December visit of Directors and Heads of Department from the Saclay centre of the French atomic energy commission (CEA) to CERN's LHC magnet test facility. Looking on is Yves Lemoigne.

  2. Experience gained at the CEA by thermal effluent utilization for agriculture and fish-breeding

    International Nuclear Information System (INIS)

    Results obtained at the CEA for waste heat utilization are examined. Water at T+100C is used for open air soil heating (forest, agriculture), eel breeding and greenhouse, heating (with the help of heat pumps). Water at T+230C or 300C is used for greenhouses. Facilities are described experience gained and results of the different exploitations are given

  3. Plasma TIMP-1 and CEA as markers for detection of primary colorectal cancer

    DEFF Research Database (Denmark)

    Christensen, Ib Jarle; Brünner, Nils; Dowell, Barry;

    2015-01-01

    endoscopy were prospectively included (N=1965). Baseline data and co-morbidity were recorded. The primary end-point was the detection of CRC. Plasma was obtained before endoscopy and TIMP-1 and CEA levels were determined using an automated analysis platform when all samples were collected. RESULTS: CRC was...

  4. 15 CFR 950.7 - Center for Environmental Assessment Services (CEAS).

    Science.gov (United States)

    2010-01-01

    ... Environmental Assessment Services (CEAS). EDIS assists National decisionmakers in solving problems by providing... experiment design, data analysis, and data management support to project managers and produces merged... data or information as required. Currently the Center is assembling an inventory of cruises and...

  5. Prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma

    Science.gov (United States)

    Wang, Wei; Chen, Xiao-Long; Zhao, Shen-Yu; Xu, Yu-Hui; Zhang, Wei-Han; Liu, Kai; Chen, Xin-Zu; Yang, Kun; Zhang, Bo; Chen, Zhi-Xin; Chen, Jia-Ping; Zhou, Zong-Guang; Hu, Jian-Kun

    2016-01-01

    The prognostic significance of preoperative serum CA125, CA19-9 and CEA in gastric carcinoma (GC) has been widely reported and is still under debate. Here, we evaluated the prognostic significance of preoperative serum CA125, CA19-9 and CEA in patients with GC. 1692 patients with GC who underwent gastrectomy were divided into the training (from January 2005 to December 2011, n = 1024) and the validation (from January 2012 to December 2013, n = 668) cohorts. Positive groups of CA125 (> 13.72 U/ml), CA19-9 (> 23.36 U/ml) and CEA (> 4.28 ng/ml) were significantly associated with more advanced clinicopathological traits and worse outcomes than that of negative groups (all P < 0.01). In Cox regression analysis, tumor size (P < 0.001, P = 0.005), pTNM stage (P < 0.001, P < 0.001) and CA125 (P = 0.026, P = 0.005) were independent prognostic factors both in two cohorts. Nomograms of these two cohorts based on the number of positive serum tumor markers (NPTM) were more accurate in prognostic prediction than TNM stage alone. Our findings suggested that elevated preoperative serum CA125, CA19-9 and CEA were associated with more advanced clinicopathological traits and less favorable outcomes. In addition, CA125 as an independent prognostic factor should be further investigated. Nomogram based on NPTM could accurately predict the prognosis of GC patients. PMID:27097114

  6. Mutational Profiles Reveal an Aberrant TGF-β-CEA Regulated Pathway in Colon Adenomas

    Science.gov (United States)

    Jogunoori, Wilma; Menon, Vipin; Majumdar, Avijit; Chen, Jiun-Sheng; Gi, Young Jin; Jeong, Yun Seong; Phan, Liem; Belkin, Mitchell; Gu, Shoujun; Kundra, Suchin; Mistry, Nipun A.; Zhang, Jianping; Su, Xiaoping; Li, Shulin; Lin, Sue-Hwa; Javle, Milind; McMurray, John S.; Rahlfs, Thomas F.; Mishra, Bibhuti; White, Jon; Rashid, Asif; Beauchemin, Nicole; Weston, Brian R.; Shafi, Mehnaz A.; Stroehlein, John R.; Davila, Marta; Akbani, Rehan; Weinstein, John N.; Wu, Xifeng; Mishra, Lopa

    2016-01-01

    Mutational processes and signatures that drive early tumorigenesis are centrally important for early cancer prevention. Yet, to date, biomarkers and risk factors for polyps (adenomas) that inordinately and rapidly develop into colon cancer remain poorly defined. Here, we describe surprisingly high mutational profiles through whole-genome sequence (WGS) analysis in 2 of 4 pairs of benign colorectal adenoma tissue samples. Unsupervised hierarchical clustered transcriptomic analysis of a further 7 pairs of adenomas reveals distinct mutational signatures regardless of adenoma size. Transitional single nucleotide substitutions of C:G>T:A predominate in the adenoma mutational spectrum. Strikingly, we observe mutations in the TGF-β pathway and CEA-associated genes in 4 out of 11 adenomas, overlapping with the Wnt pathway. Immunohistochemical labeling reveals a nearly 5-fold increase in CEA levels in 23% of adenoma samples with a concomitant loss of TGF-β signaling. We also define a functional role by which the CEA B3 domain interacts with TGFBR1, potentially inactivating the tumor suppressor function of TGF-β signaling. Our study uncovers diverse mutational processes underlying the transition from early adenoma to cancer. This has broad implications for biomarker-driven targeting of CEA/TGF-β in high-risk adenomas and may lead to early detection of aggressive adenoma to CRC progression. PMID:27100181

  7. The study of special killing effect of CD3AK on anti-CEA-positive tumor enhanced by DC loaded with CEA-rV%荷CEA-rV的DC增强CD3AK对CEA阳性肿瘤特异性杀伤作用的研究

    Institute of Scientific and Technical Information of China (English)

    祁岩超; 王新帅; 扬波

    2006-01-01

    Objective: To survey the special killing activity of CD3AK on anti-CEA-positive tumor enhanced by umbilical cord blood dendritic cell (DC) loaded with CEA recombinant vaccinia virus (CEA-rV). Methods: Freshly isolated umbilical blood mononuclear cells (UBMC) were cultivated for 3 h. Suspension cells and attached cells were used to induce CD3AK cells and DC separately. DC was loaded with CEA-rV on the 3rd day to prepare CEA-rV+DC. CD3AK cells were co-cultured with CEA-rV+DC on the 8th day, to prepare CEA-rV+DC+CD3AK. The killing activity of each effector's cell, which included UBMC, CD3AK, DC+CD3AK and CEA-rV+DC+CD3AK, was measured respectively by MTT reduction assay. Results: (1) 4 target cells were confirmed by CEA monoclonal antibody of rabbit anti-human. Lovo and A549 were really CEA positive cell lines, while Bel-7402 and K562 were CEA negative cell lines. (2) It was showed by flow-cytometry that the mature DC cultured at 10th day expressed MHCⅠ, Ⅱ molecules such as CD86, CD80, CD83 and CD40 highly, but CD123 lowly. The expression rates of CD86, CD80, CD83 and CD40 was 82.7%, 51.1%, 57.5% and 69.4%, respectively. The appearances and intra-cellular structures of DC were observed through light and electron microscope. The diameter of mature DC was 15-20 μm presented the irregular morphologic appearance, much prominences and pseudopodium. There were abundant mitochondria and endoplasmic reticulum in DC endochylema. (3) The rates of CD3, CD4, CD8 and CD28 in CD3AK cells group were 2 folds higher than that in UBMC group by FACS.It was said that the numbers of the mature T lymphocyte in CD3AK cells group were much greater than that in UBMC group. (4)The killing activities to 4 target cells of 3 effector's cells, which included CEA-rV+DC+CD3AK, DC+CD3AK and CD3AK, were much greater than that of UBMC (P<0.01). Moreover, comparing with the killing activities of 4 effector's: CEA-rV+DC+CD3AK group > DC+CD3AK group > CD3AK group > UBMC group. It showed that

  8. Dispersion of Radionuclides and Exposure Assessment in Urban Environments: A Joint CEA and LLNL Report

    Energy Technology Data Exchange (ETDEWEB)

    Glascoe, Lee [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gowardhan, Akshay [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Lennox, Kristin [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Simpson, Matthew [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Yu, Kristen [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Armand, Patrick [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Duchenne, Christophe [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Mariotte, Frederic [Alternative Energies and Atomic Energy Commission (CEA), Paris (France); Pectorin, Xavier [Alternative Energies and Atomic Energy Commission (CEA), Paris (France)

    2014-12-19

    In the interest of promoting the international exchange of technical expertise, the US Department of Energy’s Office of Emergency Operations (NA-40) and the French Commissariat à l'Energie Atomique et aux énergies alternatives (CEA) requested that the National Atmospheric Release Advisory Center (NARAC) of Lawrence Livermore National Laboratory (LLNL) in Livermore, California host a joint table top exercise with experts in emergency management and atmospheric transport modeling. In this table top exercise, LLNL and CEA compared each other’s flow and dispersion models. The goal of the comparison is to facilitate the exchange of knowledge, capabilities, and practices, and to demonstrate the utility of modeling dispersal at different levels of computational fidelity. Two modeling approaches were examined, a regional scale modeling approach, appropriate for simple terrain and/or very large releases, and an urban scale modeling approach, appropriate for small releases in a city environment. This report is a summary of LLNL and CEA modeling efforts from this exercise. Two different types of LLNL and CEA models were employed in the analysis: urban-scale models (Aeolus CFD at LLNL/NARAC and Parallel- Micro-SWIFT-SPRAY, PMSS, at CEA) for analysis of a 5,000 Ci radiological release and Lagrangian Particle Dispersion Models (LODI at LLNL/NARAC and PSPRAY at CEA) for analysis of a much larger (500,000 Ci) regional radiological release. Two densely-populated urban locations were chosen: Chicago with its high-rise skyline and gridded street network and Paris with its more consistent, lower building height and complex unaligned street network. Each location was considered under early summer daytime and nighttime conditions. Different levels of fidelity were chosen for each scale: (1) lower fidelity mass-consistent diagnostic, intermediate fidelity Navier-Stokes RANS models, and higher fidelity Navier-Stokes LES for urban-scale analysis, and (2) lower-fidelity single

  9. Development of a kit lyophilized of Anti-CEA to be labeled with Tc-99m, radionuclide obtained by extraction with MEK, complemented with studies of stability

    International Nuclear Information System (INIS)

    supports 50 mCi of Tc-99m in a maximum volume of 5 mL. The percentage of fraction immunoreactive is 78,32 % (Ab-Ag). The percentage of transquelation or dissociation face to the L-cysteine is a 5,56 % for a molar relation MoAb: Cys 1:100 and 7,05 % for 1:1000, after 4 hours of incubation to 37oC. After 24 hours it is a 10, 45 % for 1:100 and 15, 75 % for 1:1000. The biological distribution in healthy mice sacrificed after four hours, demonstrates a percentage of dose of injection in blood 55,98 %, liver 14,90 %; kidneys 3,8 %; intestines 18,07 %; in stomach and thyroid gland he is a minor of 1 %. These experimental lots of lyophilized kit present a stability of 13 months with radiochemical purity more than 93 %; and 99mTc-Anti-CEA it has a stability of up to 4 hours, post-labelling, with radiochemical purity more than 90 %. With these results, shows us that we have developed to a stable lyophilized kit of Anti-CEA to be labelled by Tc-99m (MEK), obtaining 99mTc-Anti-CEA with a high radiochemical purity more than 97 %, values acceptable and comparable with the published reports with Tc-99m, coming from a generator of adsorption. The immunoreactivity test, challenging with L-cysteine, biological distribution and study of stability have demonstrated the high quality of this lyophilized kit that keeps the capacity of biological recognition face its antigen CEA. (author)

  10. Cytokeratin 19 fragment antigen 21-1 as an independent predictor for definitive chemoradiotherapy sensitivity in esophageal squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    YAN Hong-jiang; WANG Ren-ben; ZHU Kun-li; JIANG Shu-mei; ZHAO Wei; XU Xiao-qing; FENG Rui

    2012-01-01

    Background Patients with esophageal squamous cell carcinoma (ESCC) undergoing definitive chemoradiotherapy (CRT) seem to have a disparity in therapeutic response.The identification of CRT sensitivity-related clinicopathological factors would be helpful for selecting patients most likely to benefit from CRT.Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and carcinoembryonic antigen (CEA) have been reported as useful tumor markers for esophageal cancer.The aim of this study was to examine the predictive value of CYFRA21-1 in comparison with CEA and other clinicopathological factors in patients with ESCC treated with definitive CRT.Methods Pretreatment serum CYFRA21-1 and CEA levels were measured by immunoradiometric assays.The relationships between pretreatment clinicopathological factors and the efficacy of CRT were analyzed.Overall survival (OS) was estimated by univariate and multivariate analysis.Results The results from a univariate analysis indicated that the efficacy of CRT was significantly associated with the serum levels of CYFRA21-1 and CEA before treatment (P=0.001 and P=0.023,respectively).It also indicated that the efficacy of CRT was significantly associated with the pretreatment tumor location (P=0.041).By Logistic regression analysis,the independent predictive factor associated with efficacy of CRT was CYFRA21-1 (P=0.002).The OS of the patients with high CYFRA 21-1 levels was worse than that of those with low CYFRA21-1 levels (P=0.001).In multivariate analysis,a low level of CYFRA21-1 was the most significant independent predictor of good OS (P=0.007).Conclusions CEA and tumor location may be useful in predicting the sensitivity of ESCC to CRT.CYFRA21-1 may be an independent predictor for definitive CRT sensitivity in ESCC.

  11. A Label-Free Microelectrode Array Based on One-Step Synthesis of Chitosan–Multi-Walled Carbon Nanotube–Thionine for Ultrasensitive Detection of Carcinoembryonic Antigen

    Directory of Open Access Journals (Sweden)

    Huiren Xu

    2016-07-01

    Full Text Available Carcinoembryonic antigen (CEA has been an extensively used tumor marker responsible for clinical early diagnosis of cervical carcinomas, and pancreatic, colorectal, gastric and lung cancer. Combined with micro-electro mechanical system (MEMS technology, it is important to develop a novel immune microelectrode array (MEA not only for rapid analysis of serum samples, but also for cell detection in vitro and in vivo. In this work, we depict a simple approach to modify chitosan–multi-walled carbon nanotubes–thionine (CS–MWCNTs–THI hybrid film through one-step electrochemical deposition and the CS-MWCNTs-THI hybrid films are successfully employed to immobilize anti-CEA for fabricating simple, label-free, and highly sensitive electro-chemical immune MEAs. The detection principle of immune MEA was based on the fact that the increasing formation of the antigen-antibody immunocomplex resulted in the decreased response currents and the relationship between the current reductions with the corresponding CEA concentrations was directly proportional. Experimental results indicated that the label-free MEA had good selectivity and the limit of detection for CEA is 0.5 pg/mL signal to noise ratio (SNR = 3. A linear calibration plot for the detection of CEA was obtained in a wide concentration range from 1 pg/mL to 100 ng/mL (r = 0.996. This novel MEA has potential applications for detecting CEA for the research on cancer cells and cancer tissue slices as well as for effective early diagnosis.

  12. The evaluation of the nuclear facilities safety at the CEA from 1999 to 2001; Le bilan de la surete des installations nucleaires du CEA du 1999 a 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-11-01

    The aim of this document is the presentation of an evaluation of the problems and the safety methods in the concerned period. The first chapter presents the nuclear safety in the CEA. The second chapter is devoted to the organization and the quality for the safety: liabilities, audits, relations with the safety authorities and with the public. The chapters three and four deal respectively with the methodological and technical abilities supporting the exploitation teams and with the nuclear safety projects. The last chapter presents the experiments and events from 1999 to 2001. (A.L.B.)

  13. Project: Decommissioning and Reuse of Licensed Nuclear Facilities at the CEA Centre, Fontenay-aux-Roses, (CEA-FAR) France. Annex A.I-3

    International Nuclear Information System (INIS)

    CEA-FAR is the CEA's oldest facility and is situated in the southern suburbs of Paris. It was opened on 26 March 1946 to host the first French atomic pile, which was named ZOE, and first went critical on 12 December 1946. The first laboratories were installed in existing buildings on the site. In the early 1950s, a number of nuclear facilities to be used for R and D work were built within the perimeter of the centre. This included the construction and operation of a pilot plant facility for the reprocessing of spent nuclear fuel. As space was restricted, all these installations were decommissioned and demolished to enable the construction of more modern second generation facilities. The Pu Pilot Plant for irradiated fuel reprocessing (the prototype of the Marcoule UP1 Plant) was used from 1953 to 1958. The dismantling of the equipment from the Pu Pilot Plant, the demolition of the buildings and the removal of the grounds to constitute a uniform platform were completed at the end of the 1950s. This operation made it possible to release the grounds on which building 18 was built, and also buildings 91 and 52. These buildings were constructed during the 1960s within the framework of the development of the CEN-FAR programmes. In the late 1950s and early 1960s, in addition to ZOE (NLF 11), which remained in service, the following second generation facilities were built: radio-metallurgy installations (NLF 58 and 59), radiochemistry and chemical engineering installations for the reprocessing of spent fuel (NLF 57), and installations for the treatment and interim storage of solid and liquid waste (NLF 34 and 73), which were required for pursuing the research programmes. These latter buildings are currently being cleaned up and dismantled. ZOE was closed down and partly dismantled in the 1970s to former IAEA Stage 2 and the research areas around the pile were transformed into a museum. The facilities shut down and dismantled in the 1980s were plutonium metallurgy

  14. Continuously tritium monitoring of the pipe of liquid effluents at the Cea Cadarache; Controle en continu du tritium de la conduite des effluents liquides du CEA Cadarache

    Energy Technology Data Exchange (ETDEWEB)

    Pira, Y

    2004-07-01

    This report is oriented toward the radiation protection of environment that is an essential component of radiation protection. It is necessary to detect any solid, liquid or gaseous abnormal release and to find its origin. The present study bears on a detection instrument in continuously to find tritium in liquid effluents of the Cea Cadarache. After having study the functioning principle of this device, an evaluation of its performances has been realised ( back noise, yield, detection limit) and to a checking in real conditions of utilization. (N.C.)

  15. Producing energy without greenhouse effect gases: the CEA action; Produire de l'energie sans gaz a effet de serre: l'action du CEA

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    Major actor in the domain of new energy technologies, the CEA manages the french research on the hydrogen and the fuel cells. It is also implied with INES (National Institute for the Solar Energy) in the photovoltaic and thermal solar. With the IFP (French Petroleum Institute), it manages research on biofuels. Of course the thermonuclear fusion, for the development of the energy of the future, is in its research program too. This information document presents the possibilities of these energies and the associated research programs. (A.L.B.)

  16. The CEA and alternative energies. 8 April 2010 press conference; Le CEA et les energies alternatives. Conference de presse du 8 avril 2010

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This document presents the CEA's strategy in terms of alternative energies and the various implemented research programs which mainly concern the building sector and the transport sector. After a recall of the energy and climate context, a presentation of the NTE program (Nouvelles Technologies de l'Energie, new energy technologies), the different topics and projects are presented: photovoltaic solar energy and its integration in building; batteries, hydrogen and fuel cells for applications in transports; second-generation bio-fuels.

  17. Progress of the BT-EdF-CEA project. The lithium polymer battery; Avancees du projet BT-EdF-CEA. Batterie lithium polymere

    Energy Technology Data Exchange (ETDEWEB)

    Marginedes, D.; Majastre, H. [Bollore Technologies, 29 - Quimper (France); Baudry, P.; Lascaud, S. [Electricite de France, 77 - Moret sur Loing (France). Direction des Etudes et Recherches; Bloch, D.; Lebrun, N. [CEA Grenoble, CEREM, 38 (France)

    1996-12-31

    The lithium-polymer energy storage technology requires the production of thin films of huge surface. The BT-EdF-CEA consortium has studied the various manufacturing techniques of these films and their assembly. The process was chosen according to its productivity, low expensiveness, ecological impact and energy performances with capacities reaching 40 Ah. This paper explains: the objectives and specifications of the project, the advantage of the consortium and the role of the different partners, the results (coating, dry extrusion and battery element manufacturing techniques), and the electrochemical performances of the elements. (J.S.)

  18. Crystal structure of the anti-(carcinoembryonic antigen) single-chain Fv antibody MFE-23 and a model for antigen binding based on intermolecular contacts.

    Science.gov (United States)

    Boehm, M K; Corper, A L; Wan, T; Sohi, M K; Sutton, B J; Thornton, J D; Keep, P A; Chester, K A; Begent, R H; Perkins, S J

    2000-03-01

    MFE-23 is the first single-chain Fv antibody molecule to be used in patients and is used to target colorectal cancer through its high affinity for carcinoembryonic antigen (CEA), a cell-surface member of the immunoglobulin superfamily. MFE-23 contains an N-terminal variable heavy-chain domain joined by a (Gly(4)Ser)(3) linker to a variable light-chain (V(L)) domain (kappa chain) with an 11-residue C-terminal Myc-tag. Its crystal structure was determined at 2.4 A resolution by molecular replacement with an R(cryst) of 19.0%. Five of the six antigen-binding loops, L1, L2, L3, H1 and H2, conformed to known canonical structures. The sixth loop, H3, displayed a unique structure, with a beta-hairpin loop and a bifurcated apex characterized by a buried Thr residue. In the crystal lattice, two MFE-23 molecules were associated back-to-back in a manner not seen before. The antigen-binding site displayed a large acidic region located mainly within the H2 loop and a large hydrophobic region within the H3 loop. Even though this structure is unliganded within the crystal, there is an unusually large region of contact between the H1, H2 and H3 loops and the beta-sheet of the V(L) domain of an adjacent molecule (strands DEBA) as a result of intermolecular packing. These interactions exhibited remarkably high surface and electrostatic complementarity. Of seven MFE-23 residues predicted to make contact with antigen, five participated in these lattice contacts, and this model for antigen binding is consistent with previously reported site-specific mutagenesis of MFE-23 and its effect on CEA binding.

  19. Comparison Study of Cyfra 21-1, Carcinoembryonic Antigen and Telomerase Activity between Non Small Cell and Small Cell Lung Cancer Patients

    Directory of Open Access Journals (Sweden)

    B. Alani

    2008-10-01

    Full Text Available Introduction & Objective: The roles of tumor markers in the prognosis and diagnosis of lung cancer is under investigation. The aim of this study was to examine correlation between serum levels of Carcinoembryonic Antigen (CEA and Cyfra 21-1 with telomerase activity on biopsy samples in patients with lung cancer and controls.Materials & Methods: We studied 50 malignant lung cancer patients (mean age 68.3±13.8 years and 20 normal individuals (mean age 64.9±11.4 years. Serum levels of Cyfra21-1 and CEA were measured with available enzyme immunoassay kits. Telomerase activity was measured by TRAP assay based on PCR-ELISA in lung tumor biopsy. To compare quantitative means of the two groups, t-independent and Man-Whitney analysis were applied.Results: The mean serum levels of CEA and Cyfra21-1 concentration together telomerase activity of biopsy samples in lung cancer patients were significantly higher than controls. There were a strong correlation between Cyfra21-1 and CEA, Telomerase and CEA and Telomerase and Cyfra21-1 in patient group. In patients with small cell carcinoma, the mean serum levels of Cyfra21-1 and CEA were significantly higher than non small cell carcinoma patients. Telomerase activities in biopsy samples of small cell carcinoma were significantly lower than non small cell carcinoma patients.Conclusion: It is speculated that based on this findings, telomerase activity in biopsy samples for small cell carcinoma patients and serum levels of Cyfra 21-1 and CEA are the most useful tumor markers for diagnosis of squamous and adenocarcinoma of non small cell cancers respectively.

  20. Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure

    Energy Technology Data Exchange (ETDEWEB)

    Witherspoon, L.R.; Shuler, S.E.; Alyea, K.; Husserl, F.E.

    1983-10-01

    Heat inactivation has been proposed as an alternative to perchloric acid (PCA) precipitation for the extraction of carcinoembryonic antigen (CEA) from human plasma. A commercial RIA kit using heat inactivation was examined and results compared with those obtained with PCA precipitation. Adequate sensitivity (1.5 ..mu..g CEA/I plasma), satisfactory analytical recovery of CEA added to plasma, and dilutional linearity of samples found to have elevated CEA concentrations, were demonstrated for the heat-inactivation assay. Between-assay precision was better with the heat inactivation than with the PCA assay. Although the absolute concentration of CEA estimated after heat inactivation was consistently lower than that estimated after PCA extraction of plasma specimens, there was excellent correlation between results obtained with the two methods in colon cancer patients free of disease, colon cancer patients with residual or recurrent disease, patients with benign gastrointestinal disease, and in patients with chronic renal failure. The heat-inactivation assay is an excellent alternative to the PCA assay.

  1. Carcinoembryonic antigen: assay following heat compared with perchloric acid extraction in patients with colon cancer, non-neoplastic gastrointestinal diseases, or chronic renal failure.

    Science.gov (United States)

    Witherspoon, L R; Shuler, S E; Alyea, K; Husserl, F E

    1983-10-01

    Heat inactivation has been proposed as an alternative to perchloric acid (PCA) precipitation for the extraction of carcinoembryonic antigen (CEA) from human plasma. We examined a commercial RIA kit using heat inactivation, and compared results with those obtained with PCA precipitation. Adequate sensitivity (1.5 micrograms CEA/l plasma), satisfactory analytical recovery of CEA added to plasma, and dilutional linearity of samples found to have elevated CEA concentrations, were demonstrated for the heat-inactivation assay. Between-assay precision was better with the heat inactivation than with the PCA assay. Although the absolute concentration of CEA estimated after heat inactivation was consistently lower than that estimated after PCA extraction of plasma specimens, there was excellent correlation between results obtained with the two methods in colon cancer patients free of disease, colon cancer patients with residual or recurrent disease, patients with benign gastrointestinal disease, and in patients with chronic renal failure. We conclude that the heat-inactivation assay is an excellent alternative to the PCA assay.

  2. Au-F127 strawberry-like nanospheres as an electrochemical interface for sensitive detection of carcinoembryonic antigen in real sample.

    Science.gov (United States)

    Li, Juan; Xie, Hangqing; Liu, Yuhong; Ren, Hang; Zhao, Wenbo; Huang, Xiaohua

    2015-11-01

    Nanomaterial-based signal-amplification strategies hold a great promise in realizing sensitive biological detection. A simple label-free electrochemical immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed by immobilizing anti-CEA antibodies onto the Au-F127 strawberry-like nanospheres modified glassy carbon electrode (Au-F127/GCE). The Au-F127 strawberry-like nanospheres offered a large surface and multifunctional substrate for the effective immobilization of anti-CEA and the existence of Au could accelerate electron transfer and make the electrochemical signal amplified. The Au-F127 nanocomposites and anti-CEA were characterized by transmission electron microscopy (TEM), polycrystalline electron diffraction ring pattern, ultra-violet visible (UV-vis) spectra and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were employed to verify the stepwise assembly of the immunosensor and evaluated the analytical performance of the fabricated immunosensor, respectively. The immunosensor showed a wide liner response range between 0.01 and 80 ng mL(-1) with a low detection limit of 0.24 pg mL(-1) at a signal-to-noise (S/N) ratio of 3. Additionally, the proposed method was successfully applied to determine CEA in human serum samples with satisfactory results. PMID:26452840

  3. Utility of squamous cell carcinoma antigen, carcinoembryonic antigen, Cyfra 21-1 and neuron specific enolase in lung cancer diagnosis: a prospective study from China

    Institute of Scientific and Technical Information of China (English)

    SONG Wei-an; LIU Xi; TIAN Xiao-dong; WANG Wei; LIANG Chao-yang; ZHANG Tao; GUO Jun-tang; PENG Yang-hong; ZHOU Nai-kang

    2011-01-01

    Background Early detection and diagnosis is urgent for the sake of effective treatment strategy for lung cancer.However,a convenient,economical and relatively precise method is not available.We here report a prospective study to find the possible value of the combined use of four popular tumor markers in the early diagnosis of lung cancer among patients with suspicious nodules in the lung.Methods Six hundred and sixty inpatients with suspicious nodules in the lung were divided into a lung cancer group and a benign pulmonary tumor group according to post-operative histological examinations.Serum levels of four tumor markers including squamous cell carcinoma antigen (SCC),carcinoembryonic antigen (CEA),Cyfra 21-1 and neuron specific enolase (NSE) were assayed for each patient.Receiver operating characteristic (ROC) curves were constructed for each tumor marker.The power of lung cancer diagnosis of each tumor marker,as well as a combination of them were analyzed and compared.Results The serum levels (median,range) of SCC,CEA,Cyfra 21-1 and NSE were 0.44 (0.01-35.70) ng/ml,2.49(0.30-26.78) ng/ml,2.30 (0.82-73.33) ng/ml and 10.54 (0.10-56.41) ng/ml respectively in lung cancer group,and were 0.32 (0.01-0.90) ng/ml,1.60 (0.20-8.93) ng/ml,1.41 (0.72-4.82) ng/ml and 9.36 (6.56-24.24) ng/ml respectively in the benign pulmonary tumor group.The difference in each tumor marker between the two groups was significant (P <0.05).The ROCs of SCC,CEA,Cyfra 21-1 and NSE were 0.702 (95% CI,0.654-0.751),0.611 (95% CI,0.563-0.659),0.650(95% CI,0.601-0.700) and 0.598 (95% CI,0.542-0.654) respectively,indicating very low power of these four tumor markers.When a combination of SCC,CEA,Cyfra 21-1 and NSE were employed,the diagnosis power was strengthened.Conclusion SCC,CEA,Cyfra 21-1 and NSE are valuable in the early diagnosis of lung cancer among suspicious nodules in the lung,especially when they were assayed together for one patient.

  4. DIAGNOSTIC VALUE OF SERUM VEGF, CEA AND NSE IN DIAGNOSIS OF NON-SMALL CELL LUNG CANCER%血清VEGF、CEA、NSE在非小细胞肺癌诊断中应用价值研究

    Institute of Scientific and Technical Information of China (English)

    郑立平; 林海峰

    2011-01-01

    [目的]探讨血清血管内皮生长因子(VEGF)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)在非小细胞肺癌(NSCLC)诊断中的应用价值,旨在为肺癌的诊治提供理论参考.[方法]采用ELISA方法测定100例NSCLC患者和50例健康查体者血清VEGF、CEA、NSE水平,并分析其与病理分型和临床分期的关系.[结果]肺癌组血清CEA、NSE、VEGF水平均显著高于对照组,相比较差异有统计学意义(P<0.05);鳞癌患者血清CEA和NSE水平显著高于腺癌,相比较差异有统计学意义(P<0.05);而血清VEGF水平在鳞癌和腺癌患者中差异无统计学意义(P>0.05);血清VEGF、CEA、NSE水平随着临床分期的递增而显著上升,其血清水平为Ⅳ期>Ⅲ期>I~Ⅱ期,3组之间比较差异有统计学意义(P<0.05).[结论]联合检测肺癌患者血清中VEGF、CEA、NSE水平的变化,对NSCLC的早期诊断和鉴别具有重要的临床价值.%[Objective] To explore diagnostic value of serum vascular endotbelial growth factor (VEGF), earcinoembry-onic antigen (CEA) and neuron-specific enolase (NSE) for diagnosis of the non-small cell lung cancer (NSCLC), and to provide theoretical reference for diagnosis and treatment of NSCLC. [Methods] 100 cases of patients with NSCLC and 50 cases of healthy people were determined by ELJSA, and their relationship with pathological type and clinical stage were analyzed. [ Results ] The serum CEA, NSE and VEGF levels of the lung cancer group were significantly higher than that of control group, the difference was significant (P 0.05); The serum VEGF, CEA and NSE levels significantly increased with increasing clinical stage, the difference was significant among the three groups (P< 0.05). [Conclusion] Combined detection of the serum VEGF, CEA and NSE levels has important clinical value for the early diagnosis and diagnosis of NSCLC.

  5. Adenovirus tumor targeting and hepatic untargeting by a coxsackie/adenovirus receptor ectodomain anti-carcinoembryonic antigen bispecific adapter.

    Science.gov (United States)

    Li, Hua-Jung; Everts, Maaike; Pereboeva, Larisa; Komarova, Svetlana; Idan, Anat; Curiel, David T; Herschman, Harvey R

    2007-06-01

    Adenovirus vectors have a number of advantages for gene therapy. However, because of their lack of tumor tropism and their preference for liver infection following systemic administration, they cannot be used for systemic attack on metastatic disease. Many epithelial tumors (e.g., colon, lung, and breast) express carcinoembryonic antigen (CEA). To block the natural hepatic tropism of adenovirus and to "retarget" the virus to CEA-expressing tumors, we used a bispecific adapter protein (sCAR-MFE), which fuses the ectodomain of the coxsackie/adenovirus receptor (sCAR) with a single-chain anti-CEA antibody (MFE-23). sCAR-MFE untargets adenovirus-directed luciferase transgene expression in the liver by >90% following systemic vector administration. Moreover, sCAR-MFE can "retarget" adenovirus to CEA-positive epithelial tumor cells in cell culture, in s.c. tumor grafts, and in hepatic tumor grafts. The sCAR-MFE bispecific adapter should, therefore, be a powerful agent to retarget adenovirus vectors to epithelial tumor metastases.

  6. Fusion technology. Annual report of the Association CEA/EURATOM 1997

    International Nuclear Information System (INIS)

    The research and development work performed by the French EURATOM-CEA Association for fusion technology is part of the Fusion Programme of the European Community. This report compiles the work carried out during the year 1997 as follows: The ITER CEA activities and related developments are described in the first section (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); The second part is dedicated to the Long Term activities as Blankets and material developments, long term safety, socio-economic problem; The Underlying Technology activities are compiled in the third part of this report (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); And the fourth part describes the inertial confinement studies. (K.A.)

  7. 2004 annual report. Defense, safety, energy, information, health. CEA in the center of big European challenges

    International Nuclear Information System (INIS)

    This document is the 2004 annual report of the French atomic energy commission (CEA). It presents the R and D activities of the CEA in three main domains: 1 - defense and safety, maintaining perenniality of nuclear dissuasion and nuclear safety: supplying nuclear weapons to armies, maintaining dissuasion capability with the simulation program, sharing R and D means with the scientific community and the industrial world, designing and maintaining naval nuclear propulsion reactors, cleansing Marcoule and Pierrelatte facilities, monitoring treaties and fighting against proliferation and terrorism; 2 - energy, developing more competitive and cleaner energy sources: nuclear waste management, optimization of industrial nuclear activities, future nuclear systems and new energy technologies, basic research on energy, radiobiology and toxicology; 3 - information and health, valorizing industry thanks to technological research and supplying new tools for health and medical research: micro- and nano-technologies, software technologies, basic research for industrial innovation, nuclear technologies for health and bio-technologies. (J.S.)

  8. R and D developments on the disposal of radioactive wastes recently carried out at CEA (France)

    International Nuclear Information System (INIS)

    Since the law of december 31, 1991 voted by the French Parliament, disposal in geological formations is not the sole option considered for the management of radioactive wastes. Scientists and engineers, notably within the 'Commissariat a l'Energie Atomique' (CEA), must investigate in parallel two other options, namely i. advanced separation of long-lived radioisotopes and transmutation, and ii. improvement of radioactive waste matrices and long-term storage. Still, the geological disposal of radioactive wastes, which has generated within the last decade an immense amount of Research and Development, remains a major proposed solution to the long-term management of transuranic and high-level wastes. In this paper, we will describe some of the Research and Development carried out within CEA, avoiding the industrial and engineering aspects of the issues dealt with. Rather, we will illustrate major points of our basic research, underlining in particular methodological considerations

  9. Cancer mortality of nuclear workers of CEA and COGEMA from 1969 to 1986

    International Nuclear Information System (INIS)

    Cancer mortality of the nuclear workers of CEA and COGEMA has been collected by the occupational health services of both firms from 1969 to 1986. The data are related only to the workers who died when in activity. Only very few workers left CEA and COGEMA before retirement so we consider this mortality survey as describing correctly the cancer mortality for the age groups less than 60-65 years old. Compared to the national mortality of same sex, age and calendar period, by the method of indirect standardization, the only excess observed was in the female population, linked to breast cancer mortality. The male population demonstrated a high healthy worker effect, even for cancer mortality. This study has now to be completed by an typical epidemiological cohort study in order to test cancer mortality after retirement and to discuss a possible relation with occupational exposure. (author)

  10. Diatomácea marinha Coscinodiscus wailesii Gran et Angst(BACILLARIOPHYCEAE)

    OpenAIRE

    Ascencio, Poliana G. Marson

    2012-01-01

    A diatomácea marinha cêntrica Coscinodiscus wailesii Gran et Angst, considerada uma espécie nociva, vem despertando grande interesse ecológico. Suas florações, vêm prejudicando áreas de pesca e cultivo em diferentes regiões do mundo. Entretanto essa microalga marinha é muito pouco estudada em relação ao seu crescimento celular, composição química, produção e caracterização de polissacarídeos e ácidos graxos. Sendo assim, nesse trabalho foi realizado o isolamento e o cultivo da diatomácea C. w...

  11. Fusion technology. Annual report of the Association CEA/EURATOM 1997

    Energy Technology Data Exchange (ETDEWEB)

    Magaud, P.; Le Vagueres, F

    1998-12-31

    The research and development work performed by the French EURATOM-CEA Association for fusion technology is part of the Fusion Programme of the European Community. This report compiles the work carried out during the year 1997 as follows: The ITER CEA activities and related developments are described in the first section (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); The second part is dedicated to the Long Term activities as Blankets and material developments, long term safety, socio-economic problem; The Underlying Technology activities are compiled in the third part of this report (plasma facing components, vacuum vessel and shield, magnets, remote handling, safety); And the fourth part describes the inertial confinement studies. (K.A.)

  12. Report of transparency and nuclear safety 2007 CEA Fontenay aux Roses

    International Nuclear Information System (INIS)

    This report presents the activities of the CEA Center of Fontenay aux roses for the year 2007. After many years of decommissioning and dismantling of nuclear installations, the Center is now devoted (since 2005) to the development of research programmes on biology and biomedical technologies. The actions concerning the safety, the radiation protection, the significant events, the release control and the environmental impacts and the wastes stored on the center are discussed. (A.L.B.)

  13. Integral experiments performed at CEA to improve the Pu higher isotope nuclear data: PLUTO programme

    International Nuclear Information System (INIS)

    The characteristics and main results of the experimental program PLUTO, developed at CEA to improve the Pu higher isotope nuclear data, are presented. The integral experiments performed aim at providing the information necessary to adjust the multigroup cross sections used for the design calculations. The comparison of the calculation to experiment deviations on the integral parameters before and after the cross-section adjustment show the improvement brought by the PLUTO program. 6 figures, 10 tables

  14. EURATOM-CEA association contributions to the 21st symposium on fusion technology

    International Nuclear Information System (INIS)

    The 27 contributions of EURATOM-Cea association have been gathered with 6 additional papers and 1 invited paper in this document. Most papers concern Tore-Supra and deal with the ergodic divertor, particle injection, impedance concept for ICRF antennas, low hybrid current drive, RF systems, the 118 GHz ECRH experiment, the inner first wall, improved vacuum vessel protection, pellet injection, material activation, and the CIEL project. 3 of the additional papers concern the model coil of ITER

  15. Thermal hydraulic characteristics study of prototype NET and CEA cable-in-conduit conductors (CICCs)

    Energy Technology Data Exchange (ETDEWEB)

    Maekawa, Ryuji

    1995-10-31

    The thermal hydraulic characteristics of low temperature helium in a Cable-in-Conduit Conductor (CICC) significantly affects the overall design and performance of the associated large scale superconducting magnet system. It is essential to understand the transient and steady state behavior of the helium in the conductor. Throughout the development of CICCs, the reduction of flow impedance has been one of the key factors to improving the overall pressure drop. The newly developed CICC for the ITER project has a hybrid cooling scheme: a central channel that is surrounded by bundles, for which the thermal hydraulic characteristics are not well understood. This thesis describes an experimental and analytical investigation of thermal hydraulic characteristics of low temperature helium in conventional and hybrid CICCS. Pressure drop measurements for both NET and CEA conductors have been conducted, using low temperature helium and liquid nitrogen to obtain a range of Reynolds numbers. The results are correlated with classical friction factor and Reynolds number analysis. The flow impedance reduction of the CEA conductor is described by measures of a developed flow model. Thermally induced flow in the CEA conductor has been studied with an inductive heating method. The induced velocity in the central channel is measured by a Pitot tube with steady state Reynolds number up to {approximately}7000. The transient pressure wave propagation has been recorded with pressure transducers placed equally along the conductor. The supercritical helium temperature in the central channel has been measured with the thermometer probe. However, the reduction of the central channel area significantly affects the overall thermal hydraulic characteristics of the conductor. The results suggest the importance of the central channel. A transient heat transfer experiment studied the.transverse heat transfer mechanism in the CEA conductor. The temperatures in the central channel and bundle region

  16. Differentialdiagnose beim Lungenkarzinom durch CEA, CYFRA 21-1, NSE und ProGRP

    OpenAIRE

    Gruber, Christine

    2008-01-01

    Es handelt sich um eine bizentrische, retrospektive Studie am Institut für Klinische Chemie am Klinikum Großhadern, München und an der Asklepioslungenfachklinik Gauting. Anhand von insgesamt 2749 Patienten wurde univariat und multivariat analysiert, inwieweit die Tumormarker CEA, CYFRA 21-1, NSE und ProGRP und zusätzlich die Routinelaborparameter eine Hilfestellung sowohl in der Diagnostik und Differentialdiagnostik eines Lungenrundherdes (benigne Lungenerkrankung, primäres Lungenkarzinom, Lu...

  17. EURATOM-CEA association contributions to the 21st symposium on fusion technology

    Energy Technology Data Exchange (ETDEWEB)

    Garin, P.; Grosman, A.; Beaumont, B. [and others

    2000-11-01

    The 27 contributions of EURATOM-Cea association have been gathered with 6 additional papers and 1 invited paper in this document. Most papers concern Tore-Supra and deal with the ergodic divertor, particle injection, impedance concept for ICRF antennas, low hybrid current drive, RF systems, the 118 GHz ECRH experiment, the inner first wall, improved vacuum vessel protection, pellet injection, material activation, and the CIEL project. 3 of the additional papers concern the model coil of ITER.

  18. Insights into the economic viability of a new CEA system producing hydroponic lettuce

    OpenAIRE

    Ilaslan, Gunes; White, Gerald B.; Langhans, Robert W.

    2002-01-01

    A new CEA (Controlled Environment Agriculture) system growing Boston lettuce, Lactuca Sativa cv. ‘Vivaldi’, achieves the same quality and quantity of production every day of the year. The year-round constant quantity and quality production indicate potential for success. However, our research showed that out of nine selected U.S. locations with different climatic and economic conditions, the Northeast had 25.7 percent higher production costs for the same yield. The analysis indicated that giv...

  19. Estructura factorial y fiabilidad de un cuestionario de estrategias de aprendizaje en universitarios: CEA-U

    OpenAIRE

    Martín Cabrera, Eduardo; García García, Luis Alberto; Torbay Betancor, Ángela; Rodríguez Blanco, Teresa

    2007-01-01

    Se analiza la estructura factorial y la consistencia interna del Cuestionario de Estrategias de Aprendizaje en Universitarios (CEA-U) a partir de las respuestas de 1105 estudiantes de segundo ciclo de carrera universitaria,. El cuestionario está formado por tres escalas: estrategias motivacionales, cognitivas y metacognitivas, en las que el análisis factorial exploratorio arroja once factores empíricos. En un análisis factorial de segundo orden, los once factores se agrupan en tres macrofacto...

  20. Cea strategy in the field of simulation and high-performance computing

    International Nuclear Information System (INIS)

    The full text of the publication follows. Modelling and Simulation are at the heart of research and development activities carried out by Cea. Modelling relies not only on the most advanced theories and on phenomenological experiments that ensured their tuning, but also on more instrumented experiments that deliver both the useful data for the implementation of these models and the elements required for their validation. Simulation not only covers an as close as possible representation of the physical reality, but more generally all numerical experimentation carried out with any model. In particular, there are scientific problems that cannot be solved by simulation alone. Cea makes use of simulation for understanding, for design, and for taking actions. Particle physics, climatology, the analysis and comparison of genomic sequences, the study of dynamics and folding of proteins, material physics or high temperature plasma physics illustrate the theme of simulation 'for understanding'. Designing fission or fusion reactors of the future, or nuclear weapons may use of simulation for design. Real-time simulators that aim at training operators of nuclear power plants, as well as simulations carried out to prove nuclear safety, belong to the third category. Technological evolution in the field of computers, software, or algorithms, or more generally progress made in the field of modelling have enabled Cea and others to advance significantly in regard to simulation. The hierarchization of models and multi-scale simulation techniques permit in fact the use of results stemming from milli-metric, micro-metric or even nano-metric level simulations for models that are representative of the system at the metric level. The same is true for time-scales. The rendering common place of workstations or PC-cluster based department servers, readily offering a performance of the order of a Giga-flop or tens of Giga-flops, makes it possible to meet the large majority of Cea needs. The

  1. Annual report of the Association EURATOM-Cea 2005 (full report)

    International Nuclear Information System (INIS)

    This annual report summarizes activities performed by the EURATOM-Cea association in 2005. The activities carried out in the field 'physics integration' are mainly linked to the ion cyclotron range of frequency antenna development and to the development of diagnostic components. The vacuum vessel studies have mainly focused at welding techniques and at qualification of inspection methods along the vacuum vessel inter-sector weld. On the plasma facing component side investigations have been performed on material knowledge (CuCrZr creep-fatigue studies, neutron effects on material properties of CFC, development and optimisation of Be/CuCrZr joining techniques and studies dedicated to the divertor. In the field 'magnets', EURATOM-Cea association has devoted a major part of its effort to the studies of advanced Nb3Sn strands for the toroidal field coil, and the first full size conductor sample was manufactured. Within the frame of Test Blanket Module (TBM), activities mainly concerned the improvement and completion of the TBM engineering design. Within the frame of the Helium Cooled Pebble Bed concept programmes, studies about the development of Li2TiO3 pebbles are on going. The main objective of 2005 which was to improve the shape of Li2TiO3 pebbles has been successfully achieved. EURATOM-Cea maintained significant involvement in the development of structural materials for a fusion reactor. A strong effort has been made on a program of laser detritiation associated with remote handling. (A.C.)

  2. Value of human chorionic gonadotropin compared to CEA in discriminating benign from malignant effusions.

    Science.gov (United States)

    Lamerz, R; Stoetzer, O J; Mezger, J; Brandt, A; Darsow, M; Wilmanns, W

    1999-01-01

    Human chorionic gonadotropin (HCG) is expressed in germ cell tumors and urothelial, breast, lung and colon cancers. The aim of the study was to investigate if the determination of HCG in comparison with CEA is able to discriminate between malignant and benign effusions. Effusion and partially serum samples of 61 patients with benign (g.i., heart/kidney isnuff.) and 116 patients with malignant diseases (g.i., gynec., lung, misc., CUP) were investigated. HCG was specifically determined by an IRMA using 2 monoclonal antibodies, CEA by a conventional double Ab RIA. Cytological staining was preformed using the Pappenheim-method on cytospin preparations. Significant differences (p gynec. (32.1%) gynec. (60%) pleural effusions with high specificity (90% at 5 IU/l) but low sensitivity of 31% increasing in g.i., lung and gynecologic cases, CEA a more general TM with higher sensitivity of 45% increasing in g.i., gynecologic and lung cases (sp. 100% at 9 ng/ml) both adding significantly to cytology-negative effusions. PMID:10470169

  3. Anti-CEA aptamers labeled with 99mTc: encapsulation studies in long-circulating and pH-sensitive liposomes, biodistribution and imaging

    International Nuclear Information System (INIS)

    Colorectal cancer (CRC) is one of the leading cancers and the carcinoembryonic antigen (CEA) is a tumor marker widely used in diagnosis since it is overexpressed in tumor cells. Acid nucleic aptamers with high affinity and specificity for this antigen become promising molecules for CRC diagnosis by imaging. However, due to the action of nucleases in vivo, they have been investigated for association with liposomes, such as long-circulating and pH-sensitive liposomes (SPHL) that can be destabilized in the tumor region releasing aptamers and contributing to the CRC diagnosis by scintigraphy. In this work, SpHL containing DOPE, CHEMS and mPEG2000-DSPE were characterized by analyzing mean diameter, polydispersity index and zeta potential. The anti-CEA aptamers Apt3 and Apt3-Amino were labeled with technetium-99m and the encapsulation efficiency (EE) of 99mTc-Apt3 in the SpHL by dehydration-rehydration (modified DRV) and freeze-thaw (FT) were analyzed, both in the presence of cryoprotectants. Biodistribution and scintigraphic images were performed at 1h and 4h post-injection of 99mTc-Apt3-amino, 99mTc-Apt3-SpHL or 99mTc-Apt3-amino-SpHL complexes in Balb/c healthy mice. The SpHL dispersions were homogeneous. The radiolabeling yield with technetium-99m was over 90% for all complexes. By the dehydration-rehydration method, the SpHL increased over 200% after encapsulation procedure. By the freeze-thaw method, the SpHL size increased only 13.7%. Free 99mTc-Apt3-amino showed to be cleared by renal via with high levels of radioactivity in the kidney and bladder, however, the 99mTc-Apt3-SpHL and 99mTc-Apt3-amino-SpHL clearly indicated high uptake by liver and spleen. The biodistribution of 99mTc-Apt3-SpHL showed significant uptake of radioactivity by stomach and thyroid indicating less stability of the Apt3 radiolabelling in relation to Apt3-amino. (author)

  4. Anti-CEA aptamers labeled with {sup 99m}Tc: encapsulation studies in long-circulating and pH-sensitive liposomes, biodistribution and imaging

    Energy Technology Data Exchange (ETDEWEB)

    Leonel, M.F.V.; Andrade, A.S.R. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Oliveira, M.C.; Cardoso, V.N.; Barros, A.L.B. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia

    2015-07-01

    Colorectal cancer (CRC) is one of the leading cancers and the carcinoembryonic antigen (CEA) is a tumor marker widely used in diagnosis since it is overexpressed in tumor cells. Acid nucleic aptamers with high affinity and specificity for this antigen become promising molecules for CRC diagnosis by imaging. However, due to the action of nucleases in vivo, they have been investigated for association with liposomes, such as long-circulating and pH-sensitive liposomes (SPHL) that can be destabilized in the tumor region releasing aptamers and contributing to the CRC diagnosis by scintigraphy. In this work, SpHL containing DOPE, CHEMS and mPEG{sub 2000}-DSPE were characterized by analyzing mean diameter, polydispersity index and zeta potential. The anti-CEA aptamers Apt3 and Apt3-Amino were labeled with technetium-99m and the encapsulation efficiency (EE) of {sup 99m}Tc-Apt3 in the SpHL by dehydration-rehydration (modified DRV) and freeze-thaw (FT) were analyzed, both in the presence of cryoprotectants. Biodistribution and scintigraphic images were performed at 1h and 4h post-injection of {sup 99m}Tc-Apt3-amino, {sup 99m}Tc-Apt3-SpHL or {sup 99m}Tc-Apt3-amino-SpHL complexes in Balb/c healthy mice. The SpHL dispersions were homogeneous. The radiolabeling yield with technetium-99m was over 90% for all complexes. By the dehydration-rehydration method, the SpHL increased over 200% after encapsulation procedure. By the freeze-thaw method, the SpHL size increased only 13.7%. Free {sup 99m}Tc-Apt3-amino showed to be cleared by renal via with high levels of radioactivity in the kidney and bladder, however, the {sup 99m}Tc-Apt3-SpHL and {sup 99m}Tc-Apt3-amino-SpHL clearly indicated high uptake by liver and spleen. The biodistribution of {sup 99m}Tc-Apt3-SpHL showed significant uptake of radioactivity by stomach and thyroid indicating less stability of the Apt3 radiolabelling in relation to Apt3-amino. (author)

  5. Detection of hTERT mRNA, CEA, CA19-9 in Pleural Effusion and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    Niangui Zhao; Xiaoqun Ye; Weilan Yang; Yeqing Zou; Chunsong Yan

    2008-01-01

    OBJECTIVE To explore the clinical significance of the human telomerase reverse transcriptase (hTERT) mRNA, CEA and CA19-9 in differential diagnosis of benign and malignant pleural effusions.METHODS Concentrations of CEA and CA19-9 in pleural effusions were assayed using automated chemiluminescence, and expression of hTERT mRNA was detected by RT-PCR.RESULTS The positive rates of hTERT mRNA, CEA and CA19-9 expression in the group with malignant effusions were significantly higher compared to the group with benign effusion(P < 0.05). The sensitivity (%), specificity (%) and diagnostic accordance rates (%) of the 3 tumor markers were as follows: I)hTERT mRNA: 81.8/90.5/86.1; ii) CEA: 52.3/92.9/72.1; iii) CA19-9:34.1/90.5/61.6. The positive rates of hTERT mRNA + CEA (%)expression in the pleural effusions were 97.7.CONCLUSION All of these tumor markers can be helpful for differential diagnosis of pleural effusions, hTERT mRNA had more clinical value in differentiation of the pleural efffusions.CA19-9 is unfit to be as an optimal index. The combined assay of hTERT mRNA and CEA in pleural effusions can further raise the positive detection rate of the tumor markers and can be helpful in producing a diagnosis.

  6. Evaluation of tumor markers carcinoembryonic antigen, cytokeratin 19 fragment and cancer-associated antigen 72-4 in neoplastic and non-neoplastic canine effusions differentiation

    Directory of Open Access Journals (Sweden)

    L.V. Teixeira

    2014-10-01

    Full Text Available The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1, cancer-associated antigen 72-4 (CA 72-4 and carcinoembryonic antigen (CEA in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16 and non-neoplastic (n=16 samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistical difference was found only for the CYFRA 21-1 marker. The levels were significantly higher for the neoplastic group. The lack of significance between groups for markers CA 72-4 and CEA can be explained by the presence of other diseases in the non-neoplastic group, causing elevated levels of these markers. This study concludes that CYFRA 21-1 performed well, showing good sensitivity, specificity and accuracy in the diagnosis of neoplastic effusions in dogs. However, further investigations are necessary in patients with malignancy as those with benign effusions.

  7. CEA develops organic photovoltaic cells that might replace silicon cells; La promesse du plastique. Le CEA developpe des cellules photovoltaiques organiques qui pourraient detroner le silicium

    Energy Technology Data Exchange (ETDEWEB)

    Donnars, O.

    2003-01-01

    The program ''plastic photovoltaic solar cells'' was launched in june 2002 at LCOF (laboratory of functional organic components - CEA). The aim of this program is to show the feasibility of efficient organic photovoltaic cells. At the moment the most efficient organic cells reach a conversion rate of 3,5 % compared to the average value of 16 % for silicon cells. This program sets a term of 4 years to reach a conversion rate of 5 % and an operating life of 5000 hours. Plastics (or polymers) are less expensive than silicon, are available on an unlimited basis and are environment friendly. Plastics are easy to handle and a sole technology (serigraphy) is necessary for all the stages of the fabrication process of the organic cells: serigraphy technology is used for the deposition of the substrate, of the material in which the conversion takes place, and of the protective skin. (A.C.)

  8. Lithium/polymer batteries. Safety approach of the BT-EDF-CEA project; Accumulateurs lithium/polymere. Demarche securite du projet BT-EDF-CEA

    Energy Technology Data Exchange (ETDEWEB)

    Lascaud, S.; Baudry, P. [Electricite de France, 77 - Moret sur Loing (France). Direction des Etudes et Recherches; Majastre, H. [Bollore Technologies, 29 - Quimper (France); Bloch, D. [CEAGrenoble, CEREM, 38 (France)

    1996-12-31

    The BT-EdF-CEA consortium for the development and the performance improvement of lithium/polymer batteries has carried out a safety analysis of the industrial risk and the risk for users linked with this new technology. The process chosen for the manufacturing of lithium/polymer batteries does not generate any particular risk of personnel or environmental contamination. Security tests have permitted to observe and analyze the behaviour of 4 Ah elements during thermal shocks, perforation and crushing, and during external short-circuit on 20 Ah elements. These tests demonstrate the great thermal stability and the excellent behaviour of batteries in the case of partial destruction. (J.S.) 2 refs.

  9. The relationship between chemotherapy tolerance and CA125、CEA levels of patients with advanced NSCLC%晚期NSCLC患者血清CA125、CEA水平与化疗耐受的关系及意义

    Institute of Scientific and Technical Information of China (English)

    张秀萍; 熊新华; 瞿少刚

    2011-01-01

    目的:糖类抗原125(carbohydrate antigen,CAl25)、癌胚抗原(carcinoembryonic antigen,CEA)是最早被应用于临床的肿瘤标志物.对于肺癌患者,监测它们主要用于早期诊断和疗效评价,且两者联合其他指标具有较高的灵敏度和准确性,但其对疗效的具体影响目前还不清楚.为此我们研究了晚期非小细胞肺癌(NSCLC)患者血清CA125、CEA水平及其与化疗耐受次数的关系,从化疗耐受的角度初步探讨其对化疗的影响.方法:应用回顾性分析,观察274例晚期NSCLC患者化疗前血清CA125水平、化疗耐受次数和其中110例患者血清CEA水平,计算患者CA125和CEA阳性率,并将化疗耐受次数与化疗前血清CA125、CEA水平用SPAA10.0软件进行统计学处理.结果:部分晚期NSCLC患者CA125、CEA水平升高,且CA125阳性率(62.4%)高于CEA阳性率(40%);化疗前CA125水平对NSCLC化疗的耐受次数有统计学意义(P0.05).结论:对于晚期NSCLC患者,CA125具有更高的灵敏度,且其化疗前水平在一定程度上可预测患者的化疗耐受,监测CA125比CEA更有利于对患者进行化疗耐受评估,指导临床选择治疗手段.%Objective CA125 and CEA are the first tumor markers to be used for clinical purpose. For patients with lung cancer, These markers are mainly used for early diagnosis and monitoring the efficacy of therapy, and there be high sensitivity and accuracy in combination with other tumor markers ,but their detailed therapeutic effects are unclear. So we study the relationship between chemotherapy tolerance and CA125、CEA levels of patients with advanced NSCLC to find how their impact on chemotherapy from a chemotherapeutic tolerance perspective. Methods By retrospective analysis, we observe the serum CA125 level of 274 advanced NSCLC patients before chemotherapy, tracking the number of their tolerance of chemotherapy, and gather the level of serum CEA of 110 cases among them. Then we calculated the positive rate of CA

  10. Ultrasensitive electrochemical immunoassay for CEA through host-guest interaction of β-cyclodextrin functionalized graphene and Cu@Ag core-shell nanoparticles with adamantine-modified antibody.

    Science.gov (United States)

    Gao, Jian; Guo, Zhankui; Su, Fengjie; Gao, Liang; Pang, Xuehui; Cao, Wei; Du, Bin; Wei, Qin

    2015-01-15

    A novel non-enzymatic immunoassay was designed for ultrasensitive electrochemical detection of carcino-embryonic antigen (CEA) using β-cyclodextrin functionalized Cu@Ag (Cu@Ag-CD) core-shell nanoparticles as labels and β-cyclodextrin functionalized graphene nanosheet (CD-GN) as sensor platform. CD-GN has excellent conductivity which promoted the electric transmission between base solution and electrode surface and enhanced sensitivity of immunosensor. In addition, owing to supramolecular recognition of CD-GN for the guest molecule, quite a few synthesized adamantine-modified primary antibodies (ADA-Ab1) were immobilized on the CD-GN by supramolecular host-guest interaction between CD and ADA. Cu@Ag-CD as a signal tag could be captured by ADA-modified secondary antibody (ADA-Ab2) through a host-guest interaction, leading to a large loading of Cu@Ag nanoparticles with high electrical conductivity and catalytic activity. The fabricated immunosensor exhibits excellent analytical performance for the measurement of CEA with wide range linear (0.0001-20 ng/mL), low detection limit (20 fg/mL), good sensitivity, reproducibility and stability, which provide an enormous application prospect in clinical diagnostics.

  11. AIRIX: an induction accelerator facility developed at CEA for flash radiography in detonics

    Science.gov (United States)

    Cavailler, Claude

    1999-06-01

    AIRIX is an induction linear accelerator which will be used for flash radiography in CEA/DAM. Designed to produce an X-ray dose of more than 500 Rads at 1 meter with an X-ray focal spot size diameter of less than 2 mm (LANL-CEA DAM definition), this facility consists in a 4 MeV/3.5 kA pulsed electron injector and 16 MeV induction accelerator powered by 32 high voltage generators. A prototype of this accelerator, called PIVAIR, has been studied and realized in CEA CESTA near Bordeaux. PIVAIR is a validation step for AIRIX at 8 MeV. It includes an injector (4 MeV, 3.5 kA, 60 ns) and 16 inductor cells supplied by 8 high voltage generators (250 kV, 70 ns). Two different technologies of induction cells have been tested (rexolite insulator or ferrite under vacuum). We have chosen ferrite under vacuum cells technology after comparison of results on beam transport and reliability tests. A focusing experiment at 7.2 MeV of the electron beam as been achieved during summer 1997. We have begun to produce X-rays in October 1997. A dose level of 50 Rad at 1 meter has been achieved with an X-ray spot size diameter of 3.5 to 4 mm (LANL-CEA DAM definition). Static flash radiography of very dense object have been achieved from November 97 until February 98. We have been able to test in situ new kinds of very high sensitive X- ray detectors and to check they had reached our very ambitious goals: (1) quantum efficiency at 5 MeV greater than 50% instead of 1% for luminous screens and film; (2) sensitivity less than 10 (mu) Rad (100 time more sensitive than radiographic luminous screens and films); (3) dynamic range greater than 100; (4) resolution less than 2 mm. We will present in this communication brand new kinds of detection systems, called high stopping power detectors, such as: (1) (gamma) camera with segmented thick crystal of BGO and MCP image intensifier; (2) multistep parallel plate avalanche chamber; (3) pixellized CdTe MeV photoconductor matrix. AIRIX accelerator is being

  12. Annual report of the Association EURATOM-Cea 2004 (full report)

    International Nuclear Information System (INIS)

    This annual report summarizes activities performed by the EURATOM-Cea association in 2004. The activities carried out in the field 'physics integration' are mainly linked to neutral beam developments and to the development of diagnostic components. In particular, in-situ diagnostics of the plasma facing surface have been studied. Concerning 'vessel activities', the manufacturing of the ITER primary first wall panel by HIP forming has been investigated. A dummy mock-up was produced to validate the manufacturing feasibility. A new welding process able to improve welding productivity has been investigated, it is based on a hybrid laser/TIG process called Hybrid Laser Conduction Welding. A ITER first wall mock-up has been successfully manufactured using induction brazing. In the field 'magnets', EURATOM-Cea association was involved to provide input information for establishing the final dimension details of the ITER cryo-plant. EURATOM-Cea is also involved with the design of different parts of the ITER magnet system and the fabrication of mock-ups for some critical parts of the coils. In the field 'tritium breeding and materials', activities have mainly concerned the improvement and completion of the TBM (tritium breeding module) engineering design. A new batch of 1 kilogram of Li2TiO3 pebbles with a size distribution in the range 0.6 to 0.8 mm was produced in 2004. Concerning materials, activities were focused on the EUROFER, a reduced activation martensitic steel. Activities performed in the field 'system studies' are dedicated to the power plant conceptual studies. In 2004, activities were focused on the reactor model AB, based on a helium-cooled lithium-lead blanket. (A.C.)

  13. Magnetic immunoassay coupled with inductively coupled plasma mass spectrometry for simultaneous quantification of alpha-fetoprotein and carcinoembryonic antigen in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xing; Chen, Beibei; He, Man; Zhang, Yiwen; Xiao, Guangyang; Hu, Bin, E-mail: binhu@whu.edu.cn

    2015-04-01

    The absolute quantification of glycoproteins in complex biological samples is a challenge and of great significance. Herein, 4-mercaptophenylboronic acid functionalized magnetic beads were prepared to selectively capture glycoproteins, while antibody conjugated gold and silver nanoparticles were synthesized as element tags to label two different glycoproteins. Based on that, a new approach of magnetic immunoassay-inductively coupled plasma mass spectrometry (ICP-MS) was established for simultaneous quantitative analysis of glycoproteins. Taking biomarkers of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as two model glycoproteins, experimental parameters involved in the immunoassay procedure were carefully optimized and analytical performance of the proposed method was evaluated. The limits of detection (LODs) for AFP and CEA were 0.086 μg L{sup −1} and 0.054 μg L{sup −1} with the relative standard deviations (RSDs, n = 7, c = 5 μg L{sup −1}) of 6.5% and 6.2% for AFP and CEA, respectively. Linear range for both AFP and CEA was 0.2–50 μg L{sup −1}. To validate the applicability of the proposed method, human serum samples were analyzed, and the obtained results were in good agreement with that obtained by the clinical chemiluminescence immunoassay. The developed method exhibited good selectivity and sensitivity for the simultaneous determination of AFP and CEA, and extended the applicability of metal nanoparticle tags based on ICP-MS methodology in multiple glycoprotein quantifications. - Highlights: • 4-Mercaptophenylboronic acid functionalized magnetic beads were prepared and characterized. • ICP-MS based magnetic immunoassay approach was developed for quantification of glycoproteins. • AFP and CEA were quantified simultaneously with Au and Ag NPs as element tags. • The developed method exhibited good selectivity and sensitivity for target glycoproteins.

  14. Magnetic immunoassay coupled with inductively coupled plasma mass spectrometry for simultaneous quantification of alpha-fetoprotein and carcinoembryonic antigen in human serum

    International Nuclear Information System (INIS)

    The absolute quantification of glycoproteins in complex biological samples is a challenge and of great significance. Herein, 4-mercaptophenylboronic acid functionalized magnetic beads were prepared to selectively capture glycoproteins, while antibody conjugated gold and silver nanoparticles were synthesized as element tags to label two different glycoproteins. Based on that, a new approach of magnetic immunoassay-inductively coupled plasma mass spectrometry (ICP-MS) was established for simultaneous quantitative analysis of glycoproteins. Taking biomarkers of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as two model glycoproteins, experimental parameters involved in the immunoassay procedure were carefully optimized and analytical performance of the proposed method was evaluated. The limits of detection (LODs) for AFP and CEA were 0.086 μg L−1 and 0.054 μg L−1 with the relative standard deviations (RSDs, n = 7, c = 5 μg L−1) of 6.5% and 6.2% for AFP and CEA, respectively. Linear range for both AFP and CEA was 0.2–50 μg L−1. To validate the applicability of the proposed method, human serum samples were analyzed, and the obtained results were in good agreement with that obtained by the clinical chemiluminescence immunoassay. The developed method exhibited good selectivity and sensitivity for the simultaneous determination of AFP and CEA, and extended the applicability of metal nanoparticle tags based on ICP-MS methodology in multiple glycoprotein quantifications. - Highlights: • 4-Mercaptophenylboronic acid functionalized magnetic beads were prepared and characterized. • ICP-MS based magnetic immunoassay approach was developed for quantification of glycoproteins. • AFP and CEA were quantified simultaneously with Au and Ag NPs as element tags. • The developed method exhibited good selectivity and sensitivity for target glycoproteins

  15. Increased electrocatalyzed performance through hairpin oligonucleotide aptamer-functionalized gold nanorods labels and graphene-streptavidin nanomatrix: Highly selective and sensitive electrochemical biosensor of carcinoembryonic antigen.

    Science.gov (United States)

    Wen, Wei; Huang, Jing-Yi; Bao, Ting; Zhou, Jun; Xia, Hong-Xing; Zhang, Xiu-Hua; Wang, Sheng-Fu; Zhao, Yuan-Di

    2016-09-15

    We report a triplex signal amplification strategy for sensitive biosensing of cancer biomarker by taking advantage of hairpin-shaped oligonucleotide-functionalized gold nanorods (HO-GNRs), graphene and the avidin-biotin reation. The strategy expands electrochemical detection of carcinoembryonic antigen (CEA) by using an aptamer as biosensor's recognition element and HO-GNRs as signal enhancer. To construct this biosensor, the GNR was used as a carrier of horseradish peroxidase (HRP) and HO aptamer with a biotin at the 3'-end and a thiol at the 5'-end, which amplified the electrochemical response because of a large molar ratio of HRP to HO. In the presence of target CEA, the binding reactions of CEA with the loop portions of the HOs caused HOs' loop-stem structure opened and exposed the biotins, and then HRP-GNRs-HO conjugates were captured on graphene and streptavidin modified electrodes via the reaction between the exposed biotins and preimmobilized streptavidins. The accumulation of HRP effectively catalyzed the hydrogen peroxide-mediated oxidation of o-phenylenediamine to generate an electrochemical reduction current for CEA detection. Under optimal conditions, the electrochemical biosensor exhibited a wide dynamic range of 5pgmL(-1) and 50ngmL(-1) toward CEA standards with a low detection limit of 1.5pgmL(-1) (signal-to-noise ratio of 3). The proposed biosensor accurately detected CEA concentration in 8 human serum samples from patients with lung diseases, showing excellent correlations with standard chemiluminescence immunoassay. Furthermore, these results of target DNA detection made it abundantly clear that the proposed strategy can also be extended for detection of other relative biomarkers using different functional DNA structures, which shows great prospects in single-nucleotide polymorphisms analysis, biomedical sensing and application for accurate clinical diseases diagnostic. PMID:27111123

  16. Ocular rosacea: a review Rosácea ocular: revisão

    Directory of Open Access Journals (Sweden)

    Ana Carolina Cabreira Vieira

    2012-10-01

    Full Text Available Rosacea is a prevalent chronic cutaneous disorder with variable presentation and severity. Although considered a skin disease, rosacea may evolve the eyes in 58-72% of the patients, causing eyelid and ocular surface inflammation. About one third of the patients develop potentially sight-threatening corneal involvement. Untreated rosacea may cause varying degrees of ocular morbidity. The importance of early diagnosis and adequate treatment cannot be overemphasized. There is not yet a diagnostic test for rosacea. The diagnosis of ocular rosacea relies on observation of clinical features, which can be challenging in up to 90% of patients in whom accompanying roseatic skin changes may be subtle or inexistent. In this review, we describe the pathophysiologic mechanisms proposed in the literature, clinical features, diagnosis and management of ocular rosacea, as well as discuss the need for a diagnostic test for the disease.A rosácea é uma condição cutânea crônica, que possui apresentações clínicas variáveis. Apesar de considerada uma doença dermatológica, os olhos podem ser acometidos em 58-72% dos casos, causando inflamação palpebral e da superfície ocular. Aproximadamente um terço dos pacientes desenvolve acometimento corneano, podendo causar baixa visual significativa. Diagnóstico precoce e tratamento adequado são de extrema importância, devido à significativa morbidade ocular que a doença pode causar. Não há, até o momento, um teste diagnóstico para rosácea. O diagnóstico da rosácea ocular depende da observação das manifestações clínicas, o que pode ser bastante desafiador em até 90% dos pacientes, em que os achados cutâneos são discretos ou inexistentes. Nesta revisão, descrevemos os mecanismos fisiopatológicos propostos na literatura, manifestações clínicas, diagnóstico e tratamento da rosácea ocular, assim como abordamos a necessidade de um teste diagnóstico.

  17. Development of recycled plastic composites for structural applications from CEA plastics

    Science.gov (United States)

    Bhalla, Agrim

    Plastic waste from consumer electronic appliances (CEAs) such as computer and printer parts including Polystyrene (PS), Acrylonitrile Butadiene Styrene (ABS), Polystyrene (PS) and PC/ABS were collected using handheld FTIR Spectrophotometer. The blends of these plastics with High Density Polyethylene (HDPE) are manufactured under special processing conditions in a single screw compounding injection molding machine. The blends are thermoplastics have high stiffness and strength, which may enhance the mechanical properties of HDPE like tensile modulus, ultimate tensile strength, tensile break and tensile yield. These composites have a potential to be used for the future application of recycled plastic lumber, thus replacing the traditional wood lumber.

  18. Introduction to CEA studies on future nuclear energy systems and nuclear desalination

    International Nuclear Information System (INIS)

    Nuclear energy has unique assets to meet the requirements for sustainable development in terms of economic competitiveness, safety, environmental friendliness and natural resources saving. The growth in world energy demand and future prospects of the decommissioning of existing power plants has stimulated the development of a new generation of nuclear energy systems intended to be more economically competitive, safer and more sustainable. The French Atomic Energy Commission (CEA) is strongly involved in advancing nuclear energy technology to meet future energy needs. The Generation IV Initiative provides an adequate framework to organise future R and D efforts for the development of innovative nuclear systems able to meet the requirements of a sustainable energy policy in terms of economic competitiveness, safety, environmental friendliness and natural resources saving. While covering most reactor technologies, the effort of the CEA is essentially focused on the development of a three-step set of gas-cooled systems. Besides the development of new concepts, the need to provide potable water to the expanding population in arid regions is potentially an emerging application for nuclear power. Its practical implementation requires parallel efforts to develop the needed technologies and to address specific aspects of their coupling with nuclear power plants. After a short recall on the Gen IV goals for future nuclear energy systems, the paper motivates the selection of a consistent range of high temperature gas cooled modular systems as a major focus for future studies. A preliminary overview of the reference concepts for the innovative set of gas cooled nuclear systems - namely the Very-High-Temperature Reactor (VHTR) and the Gas-Cooled Fast Reactor System (GFR) is given. Finally, the CEA's activities on nuclear desalination are quickly discussed: the EURODESAL project within the 5th R and D Framework Programme of the European Union; the TUNDESAL project, which is a

  19. Annual report of the Association EURATOM-Cea 2004 (executive summary)

    International Nuclear Information System (INIS)

    Progress in fusion technology is constant over the years and this report once again highlights a number of important steps that have been accomplished in this domain. This document is the executive summary of the full annual report, summarizing activities performed by the EURATOM-Cea association. This report has been organized into 10 issues: 1) physics integration, 2) reactor vessel, 3) plasma facing components, 4) remote handling, 5) magnets structures, 6) tritium breeding blankets, 7) structural material, 8) safety and environment, 9) system study, and 10) ITER site preparation

  20. Annual report of the Association EURATOM-Cea 2005 (executive summary)

    International Nuclear Information System (INIS)

    Progress in fusion technology is constant over the years and this report once again highlights a number of important steps that have been accomplished in this domain. This document is the executive summary of the full annual report, summarizing activities performed by the EURATOM-Cea association. This report has been organized into 10 issues: 1) physics integration, 2) reactor vessel, 3) plasma facing components, 4) remote handling, 5) magnets structures, 6) tritium breeding blankets, 7) structural material, 8) safety and environment, 9) system study, and 10) ITER site preparation

  1. Evaluation of the radiology state at the CEA/Fontenay-aux-Roses and its environment

    International Nuclear Information System (INIS)

    Since 1946 the nuclear installations followed one other at the CEA of Fontenay-aux-Roses still their gradual stop the last ten years, except two installations INB34 and INB73, necessary for the wastes management. Today these installations form the subject of a drainage program. The public opinion is regularly informed on this program since 1999. This document presents the stock of the actions realized since this date: the track keeping of the sites activities impacts on the environment and the actions realized since 1999. (A.L.B.)

  2. Murine antigen-induced arthritis.

    NARCIS (Netherlands)

    Berg, W.B. van den; Joosten, L.A.B.; Lent, P.L.E.M. van

    2007-01-01

    Antigen induced arthritis is a unilateral T-cell driven model caused by direct injection of an antigen into the knee joint of a FCA preimmunized animal. The chronicity is determined by antigen retention in avascular structures of the joint through charge mediated binding or antibody mediated trappin

  3. Increased serum carbohydrate antigen 19-9 in relapsed ductal breast carcinoma.

    Science.gov (United States)

    Papantoniou, Vassilios; Tsiouris, Spyridon; Koutsikos, John; Ptohis, Nikolaos; Lazaris, Dimitrios; Zerva, Cherry

    2006-01-01

    Increased serum carbohydrate antigen (CA) 19-9 is a quite uncommon manifestation of breast cancer both on early disease and on relapse. A 53-year-old woman with invasive ductal breast carcinoma underwent left-sided mastectomy. Two years later she palpated a subcutaneous mass at the mastectomy scar, arousing suspicion of local relapse. Surgery and histopathology revealed infiltration by breast adenocarcinoma and she was treated with chemotherapy. At that time serum tumor markers, carcinoembryonic antigen (CEA) and CA 15-3 were within normal range. Over the next six months she displayed an increase of serum CEA while serum CA 15-3 remained within normal range. In an attempt to search for a second neoplasm possibly of gastrointestinal (GI) origin, abdominal computed tomography (CT), magnetic resonance imaging (MRI), magnetic resonance cholangio-pancreatography (MRCP), endoscopy of the upper GI tract and colonoscopy were performed, as well as measurement of serum CA 19-9. While no indication of a GI neoplasm was detected, she displayed an over 10-fold increase of serum CA 19-9. The patient had also an X-ray mammography and technetium-99m hexakis-2-methoxyisobutylisonitrile ((99m)Tc-MIBI) scintimammography (SM). Whilst mammography was negative for contralateral disease recurrence, SM was suggestive of axillary lymph node involvement. Axillary lymph node dissection confirmed an extensive metastatic infiltration of these nodes by breast adenocarcinoma. Three months later serum CA 19-9 and CEA became normal. The interest of this case lies on the unexpected high serum CA 19-9 values found in a breast relapsed adenocarcinoma and in the important contribution of SM in diagnosing the axillary lymph node metastatic infiltration. PMID:16617392

  4. High preoperative and postoperative levels of carcinoembryonic antigen and CYFRA 21-1 indicate poor prognosis in patients with pathological Stage I nonsmall cell lung cancer

    Directory of Open Access Journals (Sweden)

    X Duan

    2015-01-01

    Full Text Available BACKGROUND: Serum carcinoembryonic antigen (CEA and the soluble fragment of cytokeratin 19 (CYFRA 21-1 are supposed to have a prognostic role in patients with nonsmall cell lung cancer (NSCLC after surgery, but it has not been used as an adjunct to the tumor-node-metastasis (TNM staging system to provide therapy options for patients with pathological Stage I NSCLC. This study was designed to investigate the effect of serum levels of CEA and CYFRA 21-1 before and after surgery on the prognosis of patients with Stage I NSCLC. MATERIALS AND METHODS: A retrospective review was performed regarding the medical records and follow-ups of 169 patients with Stage I NSCLC before and after surgery. The patients were divided into three groups based on levels of serum CEA and CYFRA 21-1 before and after surgery: (1 continuously normal-level groups (CEA [NN] and CYFRA 21-1 [NN] groups; (2 declined to normal-level groups (CEA [HN] and CYFRA 21-1 [HN] groups; and (3 continuously high-level groups (CEA [HH] and CYFRA 21-1 [HH] groups. Survival analysis was conducted using the Kaplan-Meier method for each group. The Chi-square or Fisher exact test was employed to compare clinical and pathologic factors at the level of P < 0.05. The prognostic factor was evaluated by the Cox proportional hazards model. RESULTS: Compared with the continuously normal-level groups, the CEA [HN] group was significantly correlated to tumor size (P = 0.011, and the CYFRA 21-1 [HN] group was significantly correlated to tumor type and pathological TNM in addition to tumor size. Five-year survivals were significantly lower (P = 0.004 in the CEA [HH] group (67.3% and the CEA [HN] group (86.5% than in the CEA [NN] group (85.7% and were significantly lower (P < 0.001 in the CYFRA 21-1 [HH] group (47.2% and the CYFRA 21-1 [HN] group (70.1% than in the CYFRA 21-1 [NN] group (90.1%. Multivariate analysis demonstrated that tumor size (21-50 mm, CEA [HH], and CYFRA 21-1 [HH] were independent

  5. Perfil de comercialização das Anonáceas nas Ceasas brasileiras

    Directory of Open Access Journals (Sweden)

    Hélio Satoshi Watanabe

    2014-01-01

    Full Text Available A quantidade de anonáceas comercializadas nas principais centrais de abastecimento está crescendo e concentrada na CEAGESP - 61%. As informações coletadas pelo SIEM da CEAGESP mostram, entre 2011 e 2012, o grande crescimento da oferta de atemoia e de graviola, respectivamente, 35% e 32%, entre 2011 e 2012, e a queda do volume de pinha - 20%, entre 2011 e 2012. A atemoia (54%, a pinha (41% e a graviola (5% são as anonáceas mais importantes comercializadas na CEAGESP. A origem é concentrada nos Estados da Bahia - pinha e graviola, e em Minas Gerais e São Paulo - atemoia. O estudo das causas da diferenciação de valor, entre lotes de atemoia de valores máximo e mínimo, de mesma classificação de tamanho, no mesmo dia, mostrou que a homogeneidade visual de tamanho é a maior responsável pela diferenciação de valor. A melhoria da seleção por tamanho é a melhor estratégia de diferenciação de valor a ser adotada pelo produtor.

  6. 2007-2008 activity report of the Association EURATOM-CEA (Full Report)

    International Nuclear Information System (INIS)

    This document summarizes the activities performed by EURATOM-CEA association in 2007-2008. The activities are various and have involved different issues like the study of dust generation processes and measurement techniques, ICRH antenna design for heating, integration studies inside the port-plug for diagnostics and in-situ divertor thermography, or the helium cooled lithium lead (HCLL) breeding blanket concept. Activities have been made to study the properties of the line defects governing the plastic behavior of iron base materials. Different options have been investigated for the superconducting magnet system. The measurement of in-vessel tritium inventory by laser induced breakdown spectroscopy (LIBS) technique has been investigated. 2007-2008 were also dedicated to the manufacture and complete tests of the AIA (Articulated Inspection Arm). AIA is designed to inspect divertor cassettes and the vacuum vessel first wall. Activities based on safety analysis or tests of ITER safety open issues have been carried out using several CEA facilities and expertise. This document is divided into 5 sections: 1) physics integration, 2) in-vessel, 3) magnet system and cryogenics, 4) tritium breeding and materials, 5) safety and environment, 6) system studies, 7) design support and procurement, 8) JET technology

  7. Aeroacoustics research in Europe: The CEAS-ASC report on 2014 highlights

    Science.gov (United States)

    Detandt, Yves

    2015-11-01

    The Council of European Aerospace Societies (CEAS) Aeroacoustics Specialists Committee (ASC) supports and promotes the interests of the scientific and industrial aeroacoustics community on an European scale and European aeronautics activities internationally. Each year the committee highlights some of the research and development projects in Europe. This paper is the 2014 issue of this collection of Aeroacoustic Highlights, compiled from informations submitted to the CEAS-ASC. The contributions are classified in different topics; the first categories being related to specific aeroacoustic challenges (airframe noise, fan and jet noise, helicopter noise, aircraft interior noise) and two last sections are respectively devoted to recent improvements and emerging techniques and to general advances in aeroacoustics. For each section, the present paper focus on accomplished projects, providing the state of the art in each research category in 2014. A number of research programmes involving aeroacoustics were funded by the European Commission. Some of the highlights from these programmes are summarised in this paper, as well as highlights funded by national programmes or by industry.

  8. Research and development performed at CEA/DMT in the field of nuclear reactors containments

    International Nuclear Information System (INIS)

    The behavior of containment is a key issue with respect to the safety of nuclear power plants. Extensive work has been performed for many years at CEA/DMT (Department of Mechanics and Technology) on this subject particularly on the behavior of containment under severe accidents. Large research and development programs have been carried out and are still under progress to handle the following problems: thermo-mechanical response on the containment under increasing pressure and temperature up to the ultimate behavior of the structure; behavior of the containment in case of hydrogen detonation or steam explosion; modelization and evaluation of containment leaking after a severe accident inducing cracks in the structure; and response of the containment to seismic loads. These studies have been carried out by means of finite element computations. The computer codes have been developed and validated using either analytical solutions or experimental results. Specific developments have been made in order to be able to solve the above mentioned problems: material models for reinforced concrete; fast dynamic algorithms for impacts; seismic analysis techniques and soil-structure interactions; and gas dynamics, shock waves and fluid-structure interaction. More recently, new programs have also been undertaken, concerning the behavior of containment after core melt and vessel break through. Work is in progress at CEA/DMT in the two following areas: conceptual studies concerning core catcher and residual power evacuation and design method development of core catcher, including numerical code development

  9. 2007-2008 activity report of the Association EURATOM-CEA (Executive summary)

    International Nuclear Information System (INIS)

    This document is the executive summary of the full report, summarizing activities performed by EURATOM-CEA association in 2007-2008. The activities are various and have involved different issues like the study of dust generation processes and measurement techniques, ICRH antenna design for heating, integration studies inside the port-plug for diagnostics and in-situ divertor thermography, or the helium cooled lithium lead (HCLL) breeding blanket concept. Activities have been made to study the properties of the line defects governing the plastic behavior of iron base materials. Different options have been investigated for the superconducting magnet system. The measurement of in-vessel tritium inventory by laser induced breakdown spectroscopy (LIBS) technique has been investigated. 2007-2008 were also dedicated to the manufacture and complete tests of the AIA (Articulated Inspection Arm). AIA is designed to inspect divertor cassettes and the vacuum vessel first wall. Activities based on safety analysis or tests of ITER safety open issues have been carried out using several CEA facilities and expertise

  10. Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas.

    Science.gov (United States)

    Ishikawa, Nobuhisa; Takano, Atsushi; Yasui, Wataru; Inai, Kouki; Nishimura, Hitoshi; Ito, Hiroyuki; Miyagi, Yohei; Nakayama, Haruhiko; Fujita, Masahiro; Hosokawa, Masao; Tsuchiya, Eiju; Kohno, Nobuoki; Nakamura, Yusuke; Daigo, Yataro

    2007-12-15

    Gene expression profile analyses of non-small cell lung carcinomas (NSCLC) and esophageal squamous cell carcinomas (ESCC) revealed that lymphocyte antigen 6 complex locus K (LY6K) was specifically expressed in testis and transactivated in a majority of NSCLCs and ESCCs. Immunohistochemical staining using 406 NSCLC and 265 ESCC specimens confirmed that LY6K overexpression was associated with poor prognosis for patients with NSCLC (P = 0.0003), as well as ESCC (P = 0.0278), and multivariate analysis confirmed its independent prognostic value for NSCLC (P = 0.0035). We established an ELISA to measure serum LY6K and found that the proportion of the serum LY6K-positive cases was 38 of 112 (33.9%) NSCLC and 26 of 81 (32.1%) ESCC, whereas only 3 of 74 (4.1%) healthy volunteers were falsely diagnosed. In most cases, there was no correlation between serum LY6K and conventional tumor markers of carcinoembryonic antigen (CEA) and cytokeratin 19-fragment (CYFRA 21-1) values. A combined ELISA for both LY6K and CEA classified 64.7% of lung adenocarcinoma patients as positive, and the use of both LY6K and CYFRA 21-1 increased sensitivity in the detection of lung squamous cell carcinomas and ESCCs up to 70.4% and 52.5%, respectively, whereas the false positive rate was 6.8% to 9.5%. In addition, knocked down of LY6K expression with small interfering RNAs resulted in growth suppression of the lung and esophageal cancer cells. Our data imply that a cancer-testis antigen, LY6K, should be useful as a new type of tumor biomarker and probably as a target for the development of new molecular therapies for cancer treatment.

  11. The new technology in high temperature gas-cooled reactor. The fifth anniversary of CEA/INET cooperation

    International Nuclear Information System (INIS)

    In 2001, CEA and INET signed the first agreement and a series of specific topics of cooperation (STC), which officially launched the cooperated program on HTGR technology between the two sides, Since 2002, INET has sent dozens of researchers to CEA on both short and long term basis to work with their French counterparts. Over the past five years, they have worked together in a variety of fields, such as materials, fuel, components and reactor physics. Besides, both sides sponsored workshops and technical visits to tackle the major problems. This volume with a collection of papers not only keeps a record of technical progress contributed by both sides, but also marks the fifth anniversary cooperation between CEA and INET. (authors)

  12. The use and evolution of the CEA research reactors; Utilisation et evolution des reacteurs de recherche du C.E.A

    Energy Technology Data Exchange (ETDEWEB)

    Rossillon, F.; Chauvez, C. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    The authors successively examine the different research reactors in use in the French C.E.A. Nuclear Centres. They trace briefly their histories, describing how they have been used up to the present, and how they have been adapted to changes in programme by means of certain modifications. They also describe the reasons which have led to the elaboration of the project for the new reactor Osiris. Zoe, the oldest reactor in the CEA, has been in service in the Centre de Fontenay-aux-Roses since 1948. It is used mainly for measurements of absorption cross-sections in graphite, and for various short irradiations which do not require high fluxes. The reactor EL 2, in service since 1952, was used for the first studies on gas cooling. It has also been widely used for the production of radioisotopes and for a large number of experiments in the fields of physics, metallurgy and physical chemistry. The ageing of certain elements of the reactor has led to the decision to close it down in the near future The reactor EL 3 has been widely used for experiments in physics and in the investigation of fuels. The possibilities of the reactor in fast neutron irradiations will be considerably improved by the adoption of a new type of core (the 'snow crystal' structure). Triton-I, a 2 MW swimming-pool reactor, is used for the most part for fast neutron and gamma irradiations. The modifications being carried out on it at present should result in an increase in the power of the reactor up to 4 or 5 MW. In a neighbouring compartment is housed Triton-II which is of the same general structure, as Triton-I, but whose maximum power is 100 kW. Triton-II is used solely for studies on shielding. Melusine, a 2 MW swimming-pool reactor, has been in use in the Centre d'Etudes Nucleaires de Grenoble since 1959. It has supported a very high programme concerned mainly with solid state physics, fundamental research into refractory fissile materials and special graphites, and the study of

  13. Label-Free Electrochemiluminescent Immunosensor for Detection of Carcinoembryonic Antigen Based on Nanocomposites of GO/MWCNTs-COOH/Au@CeO₂.

    Science.gov (United States)

    Pang, Xuehui; Li, Jianxiu; Zhao, Yongbei; Wu, Dan; Zhang, Yong; Du, Bin; Ma, Hongmin; Wei, Qin

    2015-09-01

    A high-sensitivity electrochemiluminescence (ECL) sensor was conducted to detect carcinoembryonic antigen (CEA). Nanocomposites of graphene oxide/carboxylated multiwall carbon nanotubes/gold/cerium oxide nanoparticles (GO/MWCNTs-COOH/Au@CeO2) were used as antibody carriers and sensing platforms to modify on glassy carbon electrodes (GCE). CeO2 nanoparticles were first exploited as an ECL luminescent material and the possible ECL mechanism was proposed in this work. GO/MWCNTs-COOH was used as a loading matrix for CeO2 nanoparticles because of the superior conductivity and large specific surface area. Au nanoparticles were further deposited on this matrix to attach anti-CEA and enhance the sensitivity of immunosensor. The proposed sensing platform showed excellent cathodic ECL performance and sensitive response to CEA. The effects of experimental conditions on the ECL performance were investigated. The proposed immunosensor showed the broad linear range (0.05-100 ng/mL) and the low detection limit (LOD, 0.02 ng/mL, signal-to-noise ratio = 3) according to the selected experimental conditions. The excellent analysis performance for determination of CEA in the human serum samples simplied this immunosensor displayed high sensitivity and excellent repeatability. More importantly, this conducted immunosensor broadens the use scope of CeO2 nanoparticles.

  14. Label-Free Electrochemiluminescent Immunosensor for Detection of Carcinoembryonic Antigen Based on Nanocomposites of GO/MWCNTs-COOH/Au@CeO₂.

    Science.gov (United States)

    Pang, Xuehui; Li, Jianxiu; Zhao, Yongbei; Wu, Dan; Zhang, Yong; Du, Bin; Ma, Hongmin; Wei, Qin

    2015-09-01

    A high-sensitivity electrochemiluminescence (ECL) sensor was conducted to detect carcinoembryonic antigen (CEA). Nanocomposites of graphene oxide/carboxylated multiwall carbon nanotubes/gold/cerium oxide nanoparticles (GO/MWCNTs-COOH/Au@CeO2) were used as antibody carriers and sensing platforms to modify on glassy carbon electrodes (GCE). CeO2 nanoparticles were first exploited as an ECL luminescent material and the possible ECL mechanism was proposed in this work. GO/MWCNTs-COOH was used as a loading matrix for CeO2 nanoparticles because of the superior conductivity and large specific surface area. Au nanoparticles were further deposited on this matrix to attach anti-CEA and enhance the sensitivity of immunosensor. The proposed sensing platform showed excellent cathodic ECL performance and sensitive response to CEA. The effects of experimental conditions on the ECL performance were investigated. The proposed immunosensor showed the broad linear range (0.05-100 ng/mL) and the low detection limit (LOD, 0.02 ng/mL, signal-to-noise ratio = 3) according to the selected experimental conditions. The excellent analysis performance for determination of CEA in the human serum samples simplied this immunosensor displayed high sensitivity and excellent repeatability. More importantly, this conducted immunosensor broadens the use scope of CeO2 nanoparticles. PMID:26271682

  15. Tumor markers CA19-9, CA242 and CEA in the diagnosis of pancreatic cancer: a meta-analysis

    OpenAIRE

    Zhang, Yimin; Yang, Jun; Li, Hongjuan; WU, YIHUA; Zhang, Honghe; Chen, Wenhu

    2015-01-01

    Background: Pancreatic cancer has the worst prognosis and early detection is crucial for improving patient prognosis. Therefore, we performed a meta-analysis to evaluate and compare the sensitivity and specificity of single test of CA19-9, CA242, and CEA, as well as combination test in pancreatic cancer detection. Methods: We searched PubMed, Embase, Medline, and Wanfang databases for studies that evaluated the diagnostic validity of CA19-9, CA242, and CEA between January 1990 and September 2...

  16. Search for CEA-like molecules in polymorphonuclear leukocytes of non-human primates using monoclonal antibodies.

    Science.gov (United States)

    Jantscheff, P; Indzhiia, L V; Micheel, B

    1986-01-01

    The monoclonal anti-CEA antibody ZIK-A42-A/C1 which reacts with NCA of human polymorphonuclear leukocytes was found to bind also to polymorphonuclear blood leukocytes of the following non-human primates tested: hamadryas baboon (Papio hamadryas), stump-tailed monkey (Macaca arctoides), pig-tailed monkey (Macaca nemestrina), and rhesus monkey (Macaca mulata). No binding was observed to mononuclear blood leukocytes. It was concluded that non-human primates contain CEA-like substances in their polymorphonuclear leukocytes as humans do and that these substances carry some identical epitopes.

  17. Clinical significance of CYFRA21-1 and CEA in the early prognosis of patients with locally advanced non-small cell lung cancer%CYFRA21-1和CEA对局部晚期非小细胞肺癌患者早期预后的意义

    Institute of Scientific and Technical Information of China (English)

    张丽娜; 么建立; 郝殿晋; 李洪臣; 王术艺; 张庆波

    2013-01-01

    Objective: To investigate the value of evaluation indicators of carcinoembryonic antigen (CEA) and cy-tokeratin 19 fragment( CYFRA21 - 1) as short - term efficacy in patients with advanced non - small cell lung cancer (NSCLC). Methods: The level of serum CEA and CYFRA21 - 1 were detected by electrochemoluminescence immu-noassay in 85 patients with locally advanced non - small cell lung cancer. All statistical analyses were done with software of SPSS14.0. Results:The level of CYFRA21 - 1 and CEA was associated with the therapeutic effect. The level of CEA and CYFRA21 - 1 of patients achieving partial remission (PR) or complete remission ( CR) after treatment were decreased significantly; On the contrary, the level of CEA and CYFRA21 - 1 of patients achieving SD or PD were basically stable or increased. In a multivariate analysis , the level of CEA and CYFRA21 - 1 after one cycle of chemotherapy were confirmed as independent prognostic factors for therapeutic effect. Conclusion; The level of CY-FRA21 - 1 and CEA are associated with the therapeutic effect. Patients may get better prognosis with the decreasing of serum tumor marker level. The level of CEA and CYFRA21 - 1 after one cycle of chemotherapy are confirmed as independent prognostic factors for therapeutic effect.%目的:研究癌胚抗原(CEA)和细胞角蛋白19片段(CYFRA21-1)作为晚期非小细胞肺癌(NSCLC)短期疗效评价指标的价值.方法:采用电化学发光法测定85例初治的局部晚期NSCLC患者化疗前后血清中CEA、CYFRA21-1的水平,所有统计应用SPSS14.0统计软件进行分析.结果:化疗前CEA、CYFRA21-1在治疗有效组(CR+ PR)和无效组(SD+ PD)的分布无显著性差异.二者的水平在治疗有效组的化疗后明显低于化疗前,比较有显著性差异;在无效组化疗后的表达水平高于化疗前,但比较无显著性差异.多因素分析显示化疗前低水平的血清CEA、CYFRA21-1是近期疗效的保护性因素,化疗

  18. KROTOS FCI experimental programme at CEA Cadarache: new features and status

    Energy Technology Data Exchange (ETDEWEB)

    Bonnet, J.M.; Bullado, Y.; Journeau, C.; Fouquart, P.; Piluso, P.; Sergeant, C.; Magallon, D. [CEA-Cadarache, DTN/STRI/LMA, Bat 708, BP1, 13108 Saint Paul lez Durance cedex (France)

    2005-07-01

    Full text of publication follows: KROTOS facility has been operated by the European Commission at JRC-Ispra for many years until 1999 as part of the FARO/KROTOS programme. The programme had to be stopped at JRC due to new EC priorities, and an agreement was concluded with CEA to transfer the KROTOS facility and know-how to Cadarache to continue investigating the pending FCI issues. By this agreement, CEA became owner of the facility. The main objective of the KROTOS programme at CEA Cadarache is understanding the role of melt properties in steam explosion propagation and energetics and, in particular, steam explosion behaviour of prototypical corium melts. Possible influence of physicochemical processes on explosiveness will also be explored. Actually, analysis of alumina debris produced at Ispra have shown that formation of metastable phases and chemical reaction with water at high temperature may play a significant role in enhancing heat transfer to water in the explosion phase. In order to reach the objective, steam explosion experiments are performed in well characterised conditions for a large spectrum of conditions and melt compositions of interest for both in- and ex-vessel situations. A trigger is applied as a rule. Advanced technology and instrumentation is used to reduce uncertainties on initial conditions and characterise the various phases of an explosion, with emphasis on high energy X-ray cinematography to qualify pre-mixing. This advanced instrumentation will enable the measurement of detailed variables to consolidate the qualification of the FCI codes. The use of high energy X-rays made it necessary to construct a new building to house the facility and its components. The facility should newly become operative early 2005. Main improvements with respect to Ispra concern melt delivery, hydrogen measurement and X-ray imaging. Preliminary studies have demonstrated that identification of the pre-mixture-water interface and coherent melt jet core, and

  19. Big Data solution for CTBT monitoring: CEA-IDC joint global cross correlation project

    Science.gov (United States)

    Bobrov, Dmitry; Bell, Randy; Brachet, Nicolas; Gaillard, Pierre; Kitov, Ivan; Rozhkov, Mikhail

    2014-05-01

    Waveform cross-correlation when applied to historical datasets of seismic records provides dramatic improvements in detection, location, and magnitude estimation of natural and manmade seismic events. With correlation techniques, the amplitude threshold of signal detection can be reduced globally by a factor of 2 to 3 relative to currently standard beamforming and STA/LTA detector. The gain in sensitivity corresponds to a body wave magnitude reduction by 0.3 to 0.4 units and doubles the number of events meeting high quality requirements (e.g. detected by three and more seismic stations of the International Monitoring System (IMS). This gain is crucial for seismic monitoring under the Comprehensive Nuclear-Test-Ban Treaty. The International Data Centre (IDC) dataset includes more than 450,000 seismic events, tens of millions of raw detections and continuous seismic data from the primary IMS stations since 2000. This high-quality dataset is a natural candidate for an extensive cross correlation study and the basis of further enhancements in monitoring capabilities. Without this historical dataset recorded by the permanent IMS Seismic Network any improvements would not be feasible. However, due to the mismatch between the volume of data and the performance of the standard Information Technology infrastructure, it becomes impossible to process all the data within tolerable elapsed time. To tackle this problem known as "BigData", the CEA/DASE is part of the French project "DataScale". One objective is to reanalyze 10 years of waveform data from the IMS network with the cross-correlation technique thanks to a dedicated High Performance Computer (HPC) infrastructure operated by the Centre de Calcul Recherche et Technologie (CCRT) at the CEA of Bruyères-le-Châtel. Within 2 years we are planning to enhance detection and phase association algorithms (also using machine learning and automatic classification) and process about 30 terabytes of data provided by the IDC to

  20. On 28 April, CERN welcomed Jean-Claude Petit, director of programmes of the Commissariat á l'Energie Atomique (CEA) in France, and Olivier Pagezy, finance director of the CEA.

    CERN Multimedia

    Maximilien Brice

    2005-01-01

    They visited the assembly hall for the CMS experiment, the COMPASS experiment and the test facilities for the LHC magnets. They are pictured (no 01) in the ATLAS cavern with Peter Jenni, ATLAS spokesman, and Gabriele Fioni, CEA deputy director of programmes

  1. Cancer testis antigen and immunotherapy

    Directory of Open Access Journals (Sweden)

    Krishnadas DK

    2013-04-01

    Full Text Available Deepa Kolaseri Krishnadas, Fanqi Bai, Kenneth G Lucas Department of Pediatrics, Division of Hematology/Oncology, University of Louisville, KY, USA Abstract: The identification of cancer testis (CT antigens has been an important advance in determining potential targets for cancer immunotherapy. Multiple previous studies have shown that CT antigen vaccines, using both peptides and dendritic cell vaccines, can elicit clinical and immunologic responses in several different tumors. This review details the expression of melanoma antigen family A, 1 (MAGE-A1, melanoma antigen family A, 3 (MAGE-A3, and New York esophageal squamous cell carcinoma-1 (NY-ESO-1 in various malignancies, and presents our current understanding of CT antigen based immunotherapy. Keywords: cancer testis antigens, immunotherapy, vaccine

  2. Human leucocyte antigens in tympanosclerosis.

    Science.gov (United States)

    Dursun, G; Acar, A; Turgay, M; Calgüner, M

    1997-02-01

    This study was designed to evaluate the association between certain HLA antigens and tympanosclerosis. The serum concentrations of HLA antigens were measured by a microlymphocytotoxicity technique in patients with tympanosclerosis and compared with a healthy control group. The serum levels of HLA-B35 and -DR3 were significantly higher in the patients with tympanosclerosis. This result suggests that certain types of HLA antigens may play an important role as an indicator or mediator in the pathogenesis of tympanosclerosis. PMID:9088683

  3. List of reports concerning reactor safety research of BMFT, CEA, EPRI, JSTA and USNRC

    International Nuclear Information System (INIS)

    This list reviews reports from the Federal Republic of Germany, from France, from Japan and from the United States of America concerning special Problems in the field of Reactor Safety Research. According to the cooperation of the Bundesminister fuer Forschung und Technologie (BMFT) with the Commissariat a l' Energie Atomique (CEA), the Japan Science and Technology Agency (JSTA), the Electric Power Research Institute (EPRI) and the United States Nuclear Regulatory Commission, these Reports are available in the Gesellschaft fuer Reaktorsicherheit (GRS). The list pursues the following order: Country of origin, problem area concerned, according to the Reactor Safety Research Programm of the BMFT, reporting organization. The list of reports appears quarterly. (orig./HP)

  4. GEDEON: A joint venture between research (CEA and CNRS) and industry (EDF and FRAMATOME)

    CERN Document Server

    Schapira, J P

    1999-01-01

    Nuclear waste partitioning and transmutation (P and T) are considered in France as an official line of research, in accordance with the Law of December 30, 1991 concerning research in the field of long lived and highly active nuclear waste. A research group called GEDEON (GEstion des DEchets par des Options Nouvelles) has been set up between CEA, CNRS, EDF and FRAMATOME with the aim to carry out basic research related to the use of accelerator driven subcritical systems (ADS) and of thorium as an option to reduce the waste long term impacts. In the partners agreement of GEDEON, the following subjects have been identified: spallation physics, nuclear data, subcritical neutronic studies, materials, thorium, system and scenario studies. The organization as well as the scientific program and activities of GEDEON are presented.

  5. Escala de Afrontamiento del Estrés Académico (A-CEA.

    Directory of Open Access Journals (Sweden)

    Isabel Piñeiro

    2010-01-01

    Full Text Available Assuming that the effects of stressors on students´ health, cognitive functioning and wellbeing depends on the stressors and how these are addressed, this paper attempts to prove the validity of an instrument for the specific evaluation of the coping stress strategies of university students. This work is developed in two phases. For this purpose a bank of items bringing together different coping stress strategies were elaborated and a application with a sample of university students was carried out. Results of this study suggest that the coping scale of academic stress questionnaire (Escala de Afrontamiento del Cuestionario de Estrés académico, A-CEA, that allows to differentiate between social support, cognitive reappraisal and planning, can be a useful instrument in the university scope.

  6. Microflora de Bromeliáceas do estado de Pernambuco, Brasil

    Directory of Open Access Journals (Sweden)

    Luis Tavares de Lyra

    1976-01-01

    Full Text Available O autor estudou a microflora de dois gêneros de BROMELIACEAE: Hoenbergia e Portea. As coletas do material foram feitas em seis regiões do Estado de Pernambuco; 1 Região da Mata-Úmida; 2 Região da Mata-Seca; 3 Região do Agreste Central; 4 Região do Agreste Setentrional; 5 Região do Agreste Meridional; 6 Região do Recife. As seguintes diatomáceas indicadoras de águas poluídas (espécies oligossaprobias foram encontradas nas seis regiões estudadas: Gomphonema parvulum (Kutz Grunow., Hantzschia amphioxys Grunow, Pinnularia borealis Ehr., Pinnularia microstauron (Ehr Cleve, gomphonema gracile Ehr., Nitzschia palea Kutz., Melosira roeseana Rabenh., Navicula mutica Kutz., Navicula cryptocephala Kutz., Eunotia pectinalis (Kutz Rabenh. Foram também observadas CHLOROPHYCEAS nas estações chuvosa e seca nas diversas regiões. Algumas são indicadoras de oligossaprobidade: Scenedesmus quadricauda (Turpin brebisson. Chlorococcum sp., Chlorella sp. Os fatores ecológicos e comentários referentes ás diatomáceas foram anotados no texto. A tabela I indica a frequência das diatomáceas nas seis regiões estudadas. Maior número dessas diatomáceas, registramos nas regiões do Agreste. A tabela II mostra a temperatura e pH da água de Hoenbergia e Portea em ambas as estações do ano (inverno e verão. Observamos a ocorrência de larvas de culex em Portea e hoenbergia, entretanto, raramente encontramos larvas de Anopheles. As coletas foram feitas durante as estações chuvosa e seca em Hoenbergia e Portea. Determinamos 35 espécies provenientes de 78 amostras coletadas durante o período de 26 meses.The author studied the microflora from two genera of Bromeliaceae: Hoenbergia and Portea. The material was collected in six natural regions of Pernambuco State: 1 Humid Forest Region; 2 Dry forest region; 3 Central Agreste Region; 4 Setentrional Agreste Region; 5 Meridional Agreste Region; 6 Recife Region. The following diatoms that indicate polluted

  7. Introduction to Cea studies on future nuclear energy systems and safety related research

    International Nuclear Information System (INIS)

    The growth in world energy demand and future prospects of decommissioning of existing power plants stimulate the development of a new generation of nuclear energy systems intended to be more economical, safer and more sustainable. CEA clearly support the idea that nuclear energy has unique assets to be eligible as one of the major energy sources of the sustainable development in terms of natural resources saving, economic competitiveness and public and environmental friendliness, but the possibility of development of nuclear energy is strongly linked with the public acceptance. Public acceptance of nuclear energy depends strongly on safety, and the publics perception of safety. Consequently, a major emphasis for future generations nuclear systems is to improve the safety and the robustness of the technology, and thus the public confidence in safety. (authors)

  8. TRIPOLI-4®, CEA, EDF and AREVA Reference Monte Carlo Code

    Science.gov (United States)

    2014-06-01

    This paper presents an overview of TRIPOLI-4®, the fourth generation of the 3D continuous-energy Monte Carlo code developed by the Service d'Etudes des Réacteurs et de Mathématiques Appliquées (SERMA) at CEA Saclay. The paper surveys the generic features: programming language, parallel operation, tracked particles, nuclear data, geometry, simulation modes, standard variance reduction techniques, sources, tracking and collision algorithms, tallies, sensitivity studies. Moreover, specific and recent features are also detailed: Doppler broadening of the elastic scattering kernel, neutron and photon material irradiation, advanced variance reduction techniques, Green's functions, cycle correlation correction, nuclear data management and depletion capabilities. The productivity tools (T4G, SALOME TRIPOLI, T4RootTools), the Verification & Validation process and the distribution and licensing policy are finally presented.

  9. Cea-DSM-DAPNIA-SACM contribution to IFMIF KEP phase June 2000 to December 2002

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-05-01

    The international fusion materials irradiation facility (IFMIF) requires the generation by a linear accelerator (LINAC) of 250 mA continuous current of deuterons at a nominal energy of 40 MeV. The basic approach is to provide 2 linac modules, each delivering 125 mA to a common target. The accelerators begin with a deuteron ion source and a low-energy beam transport to a radio-frequency quadrupole (RFQ), a buncher and a pre-accelerator up to 5 MeV. The key element technology phase (KEP) was initiated in 2000 with the objective of reducing some key technology risk factors. The IFMIF KEP is carried out at the Cea and it focuses on 5 issues: the ECR source, the 4-vanes RFQ design, the radio-frequency system, the DTL (drift tube linac) design, and high power diagnostics. The present report reviews progress made in the 5 issues quoted above. (A.C.)

  10. New high density MTR fuel. The CEA-CERCA-COGEMA development program

    International Nuclear Information System (INIS)

    The development of a new generation of LEU, high in density and with reprocessing capacities MTR fuel, is a key issue to provide reactor operators with a smooth operation which is necessary for a long term development of Nuclear Energy. In the RRFM'98 meeting, a joint contribution of CEA, CERCA and COGEMA presented a technical classification of the potential candidates uranium alloys. In this paper this MTR working group presents the development program of a new high density fuel. This program is composed of three main steps: Basic Data analysis and collection, Plate Tests (Irradiation and Post Irradiation Examinations) and Lead Test Assemblies (Irradiation and Post Irradiation Examinations). The goal to be reached is to make this new fuel available before the end of the present US return policy. (author)

  11. Cea-DSM-DAPNIA-SACM contribution to IFMIF KEP phase June 2000 to December 2002

    International Nuclear Information System (INIS)

    The international fusion materials irradiation facility (IFMIF) requires the generation by a linear accelerator (LINAC) of 250 mA continuous current of deuterons at a nominal energy of 40 MeV. The basic approach is to provide 2 linac modules, each delivering 125 mA to a common target. The accelerators begin with a deuteron ion source and a low-energy beam transport to a radio-frequency quadrupole (RFQ), a buncher and a pre-accelerator up to 5 MeV. The key element technology phase (KEP) was initiated in 2000 with the objective of reducing some key technology risk factors. The IFMIF KEP is carried out at the Cea and it focuses on 5 issues: the ECR source, the 4-vanes RFQ design, the radio-frequency system, the DTL (drift tube linac) design, and high power diagnostics. The present report reviews progress made in the 5 issues quoted above. (A.C.)

  12. Highly Sensitive Nanoparticle-based Multifunctional Biosensor for Antigen Detection

    Science.gov (United States)

    Siavoshi, Salome

    electrophoresis technique to assemble the cancer specific anti-PSA, mAb-2C5 and CEA coated nanoparticles to show that the nanoparticle-based biochip can successfully measure low concentrations of various antigen. The principle of operation of these biosensors is the fluorescence based ELISA. Testing results of the nanoparticle-based biochips indicate very high specificity and the detection limit 200 times smaller than the commercially available devices for antigen detection, laying the foundation for early detection of various diseases. The optimized assembly of antibody coated particles and selective assembly techniques introduced in this work provide the necessary tools for fabricating a miniaturized nanoparticle-based in-vivo multiplex biosensor. The antigen detection results show the great potential for early detection of various diseases using the fabricated in-vivo device.

  13. Perioperative cerebral ischemic complications after carotid endarterectomy without shunting. A series of 400 consecutive CEA evaluated by intraoperative monitoring and post-operative diffusion-weighted imaging

    International Nuclear Information System (INIS)

    The use of intraluminal shunting during carotid endarterectomy (CEA) remains controversial. From January 2001 to September 2010, 400 CEAs without shunting were performed under general anesthesia by 14 neurosurgeons (2 consultants, 12 trainees). We used electroencephalography (EEG) and somatosensory evoked potential (SEP) to monitor under selective burst suppression using barbiturate or propofol administration during cross-clamping. In 66 of the 400 CEAs (16.5%), intraoperative monitoring demonstrated abnormalities after cross-clamping. In 26 (6.5%) of the 400 CEAs, new areas of diffusion hyperintensity were identified postoperatively. Within 30 days, the combined mortality and morbidity (symptomatic ischemia) rate was 2.8%. Thirteen (3.3%) patients presented with TIA. CEA without shunting can be safely performed with EEG and SEP monitoring under induced hypertention and selective burst suppression. (author)

  14. Comparison between tissue and serum content of CA 125, CA 19-9, and carcinoembryonic antigen in ovarian tumors.

    Science.gov (United States)

    Breitenecker, G; Neunteufel, W; Bieglmayer, C; Kölbl, H; Schieder, K

    1989-01-01

    Tumor markers CA 125, CA 19-9, and carcinoembryonic antigen (CEA) were detected by immunohistochemistry in paraffin embedded tissue samples obtained from two different locations in 35 ovarian tumors. In addition, serum concentrations of these tumor markers were measured before cytoreductive surgery. The staining reaction was heterogeneous in different parts of the tumor as well as within the parenchyma. Of the marker positive tumors, a staining reaction was observed in both tissue samples in only 10 of 22 cases for CA 125, in eight of 13 cases for CEA, and in three of eight cases for CA 19-9. Eighty-one percent of the patients whose tumor was positive for CA 125 also showed elevated serum levels of this marker. A poor correlation was found between tissue and circulating CA 19-9 levels. CEA was detected in 28% of the tumors and seemed to be valuable only for monitoring in rare cases of ovarian cancer. For purposes of selecting a marker for monitoring of patients with ovarian carcinoma, immunohistochemistry has a predictive value for CA 125 only. In order to better define the marker expressed in a tumor, it is necessary to examine at least two samples of different parts of the malignant tissue.

  15. Detection of carcinoembryonic antigen mRNA in peritoneal washes from gastric cancer patients and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    Yan-Song Zhang; Jun Xu; Guang-Hua Luo; Rong-Chao Wang; Jiang Zhu; Xiao-Ying Zhang; Peter Nilsson-Ehle; Ning Xu

    2006-01-01

    AIM: To establish a more sensitive method for detection of free cancer cells in peritoneal washes from gastric cancer patients during surgery and to evaluate its clinical significance.METHODS: The carcinoembryonic antigen (CEA) mRNA levels in peritoneal washes from 65 cases of gastric cancer were detected by real-time RT-PCR. Peritoneal lavage cytology (PLC) was applied simultaneously to detection of free cancer cells. Negative controls included peritoneal washes from 5 cases of benign gastric disease and blood samples from 5 adult healthy volunteers.RESULTS: There was no CEA mRNA in peritoneal washes from benign gastric disease patients and in blood of adult healthy volunteers. The positive percentage of free cancer cells detected by real-time RT-PCR was 47.7% and only 12.3% by PLC. The positive rate of CEA mRNA was significantly related with serosa invasion between peritoneal metastasis and stage of gastric cancer.CONCLUSION: Real-time RT-PCR is a sensitive and rapid method for the detection of free cancer cells in peritoneal washes. The presence of free cancer cells in peritoneal washes is related to the pathologic stage of gastric cancer.

  16. Immune-scintigraphy of recurrent colorectal tumors with Tc-99m-marked monoclonal carcinoembryonic antigen antibodies

    International Nuclear Information System (INIS)

    After surgery in 24 patients aged 39-80 years with colorectal tumors, immune-scintigraphic examinations with Tc-99m carcinoembryonic antigen (CEA) MAb were carried out. Prior to scintigraphy, the levels of CEA and Ca 19-9 tumor markers were determined. In the majority of cases there was firm clinical evidence of local recurrence or metastases. Twenty-two of the 24 cases were scintigraphically positive. Local recurrence and metastases of the liver were correctly identified in 12 and four cases, respectively. It was difficult to detect widespread metastases of the peritoneum or a growth at the helum of the lung. In three cases, metastases of lymph nodes were confirmed by both surgery and scintigraphy, although the computed tomographic (CT) examinations were negative. In 20 patients, CT and immune-scintigraphic findings correlated. Most positive scans were confirmed surgically and at biopsy. In two patients, none of the diagnostic procedures showed positive results, despite clinical grounds for suspecting a further spread of the disease. A false-positive immune-scintigram was observed in a histologically confirmed case of inflammation of the mucous membrane. In several cases Tc-99m enhancement was detected in almost all parts of the colon. This could be due to the antibody metabolism or to the deposit of free technetium in the bowel. There was no correlation between CEA serum levels and a positive immune-scan

  17. Gnatophyma: a rare form of rosacea Gnatofima: uma forma rara de rosácea

    Directory of Open Access Journals (Sweden)

    Ana Carolina Lisboa de Macedo

    2012-12-01

    Full Text Available Phyma is the last stage of rosacea and is due to chronic inflammation and edema. It can affect nose (rhinophyma, chin (gnatophyma, forehead (metophyma, ears (otophyma and eyelids (blepharophyma. Rhinophyma is the most frequent location and there are few reports about gnatophyma. We report the case of a female patient, 41 years old, who had an infiltrated, erythematous, edematous plaque around the chin and lower lip for two years. Histopathology showed perivascular lymphocytic infiltrate, hypertrophied follicles and sebaceous glands, dilated vessels and fibrosis. She was treated with oral tetracycline, oral ivermectin and metronidazole cream with a satisfactory response. The clinical, histopathological and therapeutic response correlation confirmed the diagnosis of gnatophyma, a rare variant of phyma.Fima é o estágio final da rosácea e ocorre devido ao edema e inflamação crônica. Pode acometer nariz (rinofima, mento (gnatofima, fronte (metofima, orelhas (otofima e pálpebras (blefarofima. Rinofima é a localização mais encontrada e há raros relatos de gnatofima. Relataremos paciente feminina, 41 anos, que apresentava placa infiltrada, eritêmato-edematosa, em todo o mento e lábio inferior há dois anos. Histopatológico com infiltrado linfocitário perianexial e perivascular, folículos e glândulas sebáceas hipertrofiadas, vasos ectasiados e fibrose perianexial. Foi instituído tratamento com tetraciclina via oral, ivermectina via oral e metronidazol creme com resposta satisfatória. Através da correlação clínica, histopatológica e resposta terapêutica confirmou-se o diagnóstico da variante rara de fima, gnatofima.

  18. Colecta y establecimiento de anonáceas en El Salvador

    Directory of Open Access Journals (Sweden)

    Eduardo Cruz

    2000-01-01

    Full Text Available El Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA, a través de la Unidad de Recursos Fitogenéticos, inició en 1998 un diagnóstico geográfico de especies de anonáceas, con el objetivo de explorar, recolectar, caracterizar y establecer colecciones de campo en las Estaciones Experimentales. Las características evaluadas para frutos fueron: peso, largo y diámetro, número de semillas, textura de cáscara y pulpa; forma de fruto y cárpelos, daño por plagas y enfermedades y un análisis bromatológico. Como resultado de este trabajo se estableció la colección de anonáceas con las siguientes especies: squamosa, cherimola, reticulata, muricata, purpurea, diversifolia, holosericea y glabra. En la caracterización morfológica preliminar Annona muricata obtuvo un promedio de 175 semillas/fruto y 1,319 g en peso del fruto. A. cherimola con pulpa muy arenosa y carpelos no prominentes. A. squamosa con pulpa dulce, blanca y cárpelos muy prominentes. De la caracterización bromatológica se determinó que Annona muricata dio contenidos de humedad superiores a 82,8%; fibra cruda de 1,84% y fósforo con 367 ppm. En Annona diversifolia con menor grasa 0,01%. La mayor incidencia por plagas en frutos y semillas fue causado por el perforador Bephrateloides sp. La antracnosis (Colletotrichum sp. en frutos y follaje fue la enfermedad más observada

  19. Las Podocarpáceas de los bosques montanos del noroccidente peruano

    Directory of Open Access Journals (Sweden)

    Edgar E. Vicuña-Miñano

    2013-05-01

    Full Text Available Los bosques montanos del noroccidente peruano son formaciones vegetales que presentan una alta diversidad y endemismos. Lamentablemente, en muchos lugares están sujetos a procesos acelerados de deterioro por la deforestación y colonización, aspecto que los convierte en uno de los ecosistemas más amenazados por el hombre. Lo que ahora queda de estos bosques, alguna vez extensos, son sólo fragmentos. Los bosques montanos relictos son ecosistemas ubicados por encima de los 1000 m de altitud, en la Vertiente Occidental de los Andes. Es en estos bosques, donde se pueden encontrar especies de coníferas nativas de nuestro país, pertenecientes a la familia Podocarpaceae. Estas especies juegan un rol de suma importancia en la dinámica de estos ecosistemas tan frágiles; no obstante, debido a la calidad de sus maderas, están siendo extraídas de manera alarmante. En el noroccidente peruano existen zonas de bosques homogéneos de Podocarpáceas tal es el caso del bosque de Cachil en la provincia de Contumazá, bosque de TongodQuellahorco en la provincia de San Miguel (en los cuales predomina la especie Podocarpus oleifolius y los bosques de San Ignacio en Cajamarca, en los que reportamos cinco especies de Podocarpáceas distribuidas en tres géneros: Podocarpus (P. oleifolius, P. macrostachys, P sprucei, Prumnopitys (P. harmsiana y Nageia (N. rospigliosii, las cuales representan el 50% del total de especies reportadas para el Perú.

  20. Comparing Prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins Glypican-3, Alpha feto protein and Carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients

    OpenAIRE

    Iman A. Abd El Gawad; Mossallam, Ghada I.; Noha H. Radwan; Elzawahry, Heba M; Niveen M. Elhifnawy

    2014-01-01

    Background: Hepatocellular carcinoma (HCC) is usually asymptomatic in the early stage and does not show elevated alpha-feto protein (AFP). AFP shows 60–80% sensitivity in diagnosing HCC. Glypican3 (GPC-3) is an oncofetal protein that is only detected in HCC cells but not in benign liver tissues, while Carcinoembryonic antigen (CEA) is expressed in various neoplasms including HCC. Although, it is not specific for HCC. Prothrombin induced by vitamin K absence-II (PIVKA-II) is an abnormal ...

  1. Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells

    Directory of Open Access Journals (Sweden)

    Lee HC

    2013-06-01

    Full Text Available Hsin-chung Lee,1,2 Qing-Dong Ling,1,3 Wan-Chun Yu,4 Chunh-Ming Hung,4 Ta-Chun Kao,4 Yi-Wei Huang,4 Akon Higuchi3–51Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taoyuan, 2Department of Surgery, Cathay General Hospital, Da'an District, Taipei, 3Cathay Medical Research Institute, Cathay General Hospital, Hsi-Chi City, Taipei, 4Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, Taiwan; 5Department of Reproduction, National Research Institute for Child Health and Development, Okura, Tokyo, JapanPurpose: We evaluated the higher levels of carcinoembryonic antigen (CEA secreted by the LoVo human colon carcinoma cells in a medium containing anticancer drugs. Drug-resistant LoVo cells were analyzed by subcutaneously xenotransplanting them into mice. The aim of this study was to evaluate whether the drug-resistant cells isolated in this study were cancer-initiating cells, known also as cancer stem cells (CSCs.Methods: The production of CEA was investigated in LoVo cells that were cultured with 0–10 mM of anticancer drugs, and we evaluated the increase in CEA production by the LoVo cells that were stimulated by anticancer drug treatment. The expression of several CSC markers in LoVo cells treated with anticancer drugs was also evaluated. Following anticancer drug treatment, LoVo cells were injected subcutaneously into the flanks of severe combined immunodeficiency mice in order to evaluate the CSC fraction.Results: Production of CEA by LoVo cells was stimulated by the addition of anticancer drugs. Drug-resistant LoVo cells expressed lower levels of CSC markers, and LoVo cells treated with any of the anticancer drugs tested did not generate tumors within 8 weeks from when the cells were injected subcutaneously into severe combined immunodeficiency mice. These results suggest that the drug-resistant LoVo cells have a smaller population of CSCs than the

  2. Comparison of CYFRA 21-1, CEA, CA 19-9 and CA 72-4 levels in gastric cancer

    Directory of Open Access Journals (Sweden)

    Gökhan Çakırca

    2013-01-01

    Full Text Available Objective: Gastric cancer is the fourth most common malignancy,and the second most common cause of cancermortality worldwide. The aim of this study was to investigatethe role of biomarkers CYFRA 21-1, CA 19-9, CEA,CA72.4 at diagnosis and throughout the follow-up in patientswith gastric cancer.Methods: 30 patients with gastric cancer diagnosed and31 healthy people as a control group were included in thisstudy. According to the TNM staging system, there were16 patients with stage II and 14 patients with stage III inthe group of patients with gastric cancer. CEA, CA 19-9,CA 72-4 and CYFRA 21-1 levels were studied by elektrochemiluminescenceimmunasay (ECLIA method in theCobas e 601 instrument.Results: Statistically the level of CA 72-4, CA 19-9, CEAand CYFRA 21-1 gastric cancer groups was found significanthigh accaording to healthy control group (p<0.01.Statistically compared with II stage patient group, the levelof CA 19-9, CEA and CYFRA 21-1 of III stage patientgroup was found significant high (p<0.01. The diagnosticcut-off, sensitivity and specificity for CEA were 4.15 ng/mL, 46 % and 96%, respectively; for CA 19-9 were 24.50U/mL, 17 % and 96 %; for CA 72-4 were 2.46 U/mL, 53% and 96% and for CYFRA 21-1 were 3.36 ng/mL, 46%and 100%.Conclusion: Our study demonstrates that the associationof biomarkers CYFRA 21-1, CEA, and CA72.4 providesa valuable contribution in the follow-up of gastriccancer patients.Key words: Gastric cancer, tumor markers, CYFRA 21-1

  3. Antigen antibody interactions

    CERN Document Server

    DeLisi, Charles

    1976-01-01

    1. 1 Organization of the Immune System One of the most important survival mechanisms of vertebrates is their ability to recognize and respond to the onslaught of pathogenic microbes to which they are conti- ously exposed. The collection of host cells and molecules involved in this recognition­ 12 response function constitutes its immune system. In man, it comprises about 10 cells 20 (lymphocytes) and 10 molecules (immunoglobulins). Its ontogenic development is c- strained by the requirement that it be capable of responding to an almost limitless variety of molecular configurations on foreign substances, while simultaneously remaining inert to those on self components. It has thus evolved to discriminate, with exquisite precision, between molecular patterns. The foreign substances which induce a response, called antigens, are typically large molecules such as proteins and polysaccharides. The portions of these with which immunoglobulins interact are called epitopes or determinants. A typical protein epitope m...

  4. Clinical value of CEA and CYFRA21-1 for the differentiation of benign and malignant pleural effusion by Meta analysis%CEA和CYFRA 21-1测定鉴别胸腔积液良恶性的Meta分析

    Institute of Scientific and Technical Information of China (English)

    顾平; 陈虞梅; 黄钢; 朱翠英; 袁济民

    2008-01-01

    目的 用Meta分析方法系统评价近10年来癌胚抗原(CEA)和细胞角蛋白19片段抗原21-1(CYFRA 21-1)在鉴别良恶性胸腔积液中的价值,并进一步比较两者在诊断效能上的差异.方法 收集1996年1月-2007年1月间国内外公开发表的关于CEA和CYFRA 21-1鉴别良恶性胸腔积液的文献,对纳入文献进行方法学质量评估后,运用Q检验评价数据的异质性,合并其灵敏度和特异性,用两者综合受试者工作特征(SROC)曲线下面积(AUC)评价和比较(Z检验)CEA和CYFRA 21-1鉴别良恶性胸腔积液的效能.结果 共有19篇文献纳入,研究对象3228例.CEA汇总的灵敏度和特异性[95%可信区间(CI)]为45.9%(43.2%~48.5%)和97.0%(96.0%~97.8%), CYFRA 21-1汇总的灵敏度和特异性(95%CI)为47.3%(44.0%~50.6%)和91.8%(89.5%~93.7%),其结果均存在明显的异质性(P<0.05).CEA和CYFRA 21-1的AUC分别为0.7691和0.8213,Z检验比较两者AUC差异无统计学意义(Z=0.45,P>0.05).结论 CEA和CYFRA 21-1在诊断良恶性胸腔积液时均具有较好的准确性.%Objective Pleural effusion is a common clinical complication caused by many benign or malignant diseases. Many published papers presented that tumor-marker assays, such as carcinoembryonic antigen (CEA) and cytokeratin 19 fragment antigen 21-1 (CYFRA21-1), played a vital role on the benign and malignant differentiation. This study aimed to reviewe currently available published papers to evaluate the clinical value of those two markers for the differentiation of benign and malignant pleural effusion. Methods All published data of pleural effusion CEA and CYFRA21-1 for the differentiation studies from year 1996 to 2007 were reviewed. By methodological qualities and Q test, 19 papers were selected into this study. The overall diagnostic sensitivity, specificity, and area under curve (AUC) from receiver operating characteristic (ROC) curve were adopted to compare the value of pleural effusion CEA and CYFRA21-1 for

  5. Measurement of serum carcinoembryonic antigen, carbohydrate antigen 19-9, cytokeratin-19 fragment and matrix metalloproteinase-7 for detecting cholangiocarcinoma: a preliminary case-control study.

    Science.gov (United States)

    Lumachi, Franco; Lo Re, Giovanni; Tozzoli, Renato; D'Aurizio, Federica; Facomer, Flavio; Chiara, Giordano B; Basso, Stefano M M

    2014-11-01

    Cholangiocarcinoma is a malignant tumor of the liver arising from the bile duct epithelium, accounting for 10-25% of all primary hepatic cancers. The clinical presentation of this tumor is not specific and the diagnosis of early cholangiocarcinoma is difficult, especially in patients with other biliary diseases. Measurement of serum carbohydrate antigen (CA) 19-9 and carcinoembryonic antigen (CEA) are commonly used to monitor response to therapy, but are also useful for confirming the presence of a cholangiocarcinoma. In this setting, other biomarkers have been previously tested, including cytokeratin-19 fragment (CYFRA 21-1) and the matrix metalloproteinase-7 (MMP7). The purpose of this retrospective study was to determine the clinical usefulness of the assay of serum CEA, CA 19-9, CYFRA 21-1 and MMP7, individually and together, as tumor markers for the diagnosis of cholangiocarcinoma. Twenty-four patients (14 men, 10 women, 62.6±8.2 years of age) with histologically-confirmed cholangiocarcinoma (cases) and 25 age- and sex-matched patients with benign liver disease (controls) underwent measurement of these biomarkers. The mean values of all serum markers of patients with cholangiocarcinoma were significantly higher (pCYFRA 21-1: 76%, 79% and 78%; MMP7: 78%, 77% and 80%, respectively. The combination of all serum markers afforded 92.0% sensitivity and 96% specificity in detecting cholangiocarcinoma, showing the highest diagnostic accuracy (94%). In conclusion, our preliminary results suggest that the measurement of all four biomarkers together can help in the early detection of cholangiocarcinoma. PMID:25368272

  6. Binding of insecticidal lectin Colocasia esculenta tuber agglutinin (CEA) to midgut receptors of Bemisia tabaci and Lipaphis erysimi provides clues to its insecticidal potential.

    Science.gov (United States)

    Roy, Amit; Gupta, Sumanti; Hess, Daniel; Das, Kali Pada; Das, Sampa

    2014-07-01

    The insecticidal potential of Galanthus nivalis agglutinin-related lectins against hemipterans has been experimentally proven. However, the basis behind the toxicity of these lectins against hemipterans remains elusive. The present study elucidates the molecular basis behind insecticidal efficacy of Colocasia esculenta tuber agglutinin (CEA) against Bemisia tabaci and Lipaphis erysimi. Confocal microscopic analyses highlighted the binding of 25 kDa stable homodimeric lectin to insect midgut. Ligand blots followed by LC MS/MS analyses identified binding partners of CEA as vacuolar ATP synthase and sarcoplasmic endoplasmic reticulum type Ca(2+) ATPase from B. tabaci, and ATP synthase, heat shock protein 70 and clathrin heavy chain assembly protein from L. erysimi. Internalization of CEA into hemolymph was confirmed by Western blotting. Glycoprotein nature of the receptors was identified through glycospecific staining. Deglycosylation assay indicated the interaction of CEA with its receptors to be probably glycan mediated. Surface plasmon resonance analysis revealed the interaction kinetics between ATP synthase of B. tabaci with CEA. Pathway prediction study based on Drosophila homologs suggested the interaction of CEA with insect receptors that probably led to disruption of cellular processes causing growth retardation and loss of fecundity of target insects. Thus, the present findings strengthen our current understanding of the entomotoxic potentiality of CEA, which will facilitate its future biotechnological applications.

  7. Binding of insecticidal lectin Colocasia esculenta tuber agglutinin (CEA) to midgut receptors of Bemisia tabaci and Lipaphis erysimi provides clues to its insecticidal potential.

    Science.gov (United States)

    Roy, Amit; Gupta, Sumanti; Hess, Daniel; Das, Kali Pada; Das, Sampa

    2014-07-01

    The insecticidal potential of Galanthus nivalis agglutinin-related lectins against hemipterans has been experimentally proven. However, the basis behind the toxicity of these lectins against hemipterans remains elusive. The present study elucidates the molecular basis behind insecticidal efficacy of Colocasia esculenta tuber agglutinin (CEA) against Bemisia tabaci and Lipaphis erysimi. Confocal microscopic analyses highlighted the binding of 25 kDa stable homodimeric lectin to insect midgut. Ligand blots followed by LC MS/MS analyses identified binding partners of CEA as vacuolar ATP synthase and sarcoplasmic endoplasmic reticulum type Ca(2+) ATPase from B. tabaci, and ATP synthase, heat shock protein 70 and clathrin heavy chain assembly protein from L. erysimi. Internalization of CEA into hemolymph was confirmed by Western blotting. Glycoprotein nature of the receptors was identified through glycospecific staining. Deglycosylation assay indicated the interaction of CEA with its receptors to be probably glycan mediated. Surface plasmon resonance analysis revealed the interaction kinetics between ATP synthase of B. tabaci with CEA. Pathway prediction study based on Drosophila homologs suggested the interaction of CEA with insect receptors that probably led to disruption of cellular processes causing growth retardation and loss of fecundity of target insects. Thus, the present findings strengthen our current understanding of the entomotoxic potentiality of CEA, which will facilitate its future biotechnological applications. PMID:24753494

  8. 血清p53抗体联合CEA、CYFRA21-1检测在肺癌诊断中的价值%Role of combined detection of serum anti-p53 antibody with CEA and CYFRA21-1 in the diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    杜紫燕; 余勇; 张增利; 李凌云; 邢玉斐; 施敏骅

    2013-01-01

    目的 探讨联合检测肺癌患者血清p53抗体与癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)辅助肺癌诊断的可行性.方法 在57例肺癌(肺癌组)和40例肺部良性疾病患者(对照组),采用ELISA法检测血清p53抗体,采用电化学发光免疫分析法检测血清CEA和CYFRA21-1.结果 肺癌组血清p53抗体、CEA和CYFRA21-1水平均高于对照组(P<0.05).p53抗体、CEA、CYFRA21-1曲线下面积(AUC)分别为0.770、0.814、0.779.p53抗体、CEA和CYFRA21-1的诊断界点分别为1.150 U/ml、3.095 ng/ml、3.240 ng/ml,敏感性分别为45.61%、66.67%、68.42%,特异性分别为87.50%、87.50%、85.00%.三项联合检测的AUC为0.902,敏感性为89.47%,特异性为100%.CEA与CYFAR21-1呈正相关(r=0.555,P<0.05).结论 血清p53抗体为独立的肺癌生物标记物,与CEA、CYFRA21-1联合检测有助于提高肺癌诊断水平.%Objective To explore the value of combined detection of serum anti-p53 antibody with carcinoembryonic antigen(CEA) and CYFRA21-1 in the diagnosis of lung cancer. Methods In 57 patients with lung cancer (group A) and 40 patients with benign pulmonary diseases (group B), serum levels of anti-p53 antibody, CEA and CYFRA 21-1 were measured by ELISA and electro-chemiluminescence immunoassay. Results Serum levels of anti-p53 antibody, CEA and CYFRA21-1 were higher in group A than those in group B(P<0. 05). The values of receiver operating characteristic area under the curve(AUC) for anti-p53 antibody,CEA and CYFRA 21-1 were 0. 770, 0. 814 and 0. 779, respectively. The cutoff levels of anti-p53 antibody, CEA and CYFRA 21-1 were 1. 150 U/ml,3. 095 ng/ml and 3. 240 ng/ml, respectively. The sensitivities of those markers for lung cancer detection were 45. 61%,66. 67%, 68. 42%, while the specificities were 87. 50%, 87. 50% and 85. 00% respectively. The value of AUC, sensitivity and specificity were 0. 90, 89. 47% and 100%, respectively,when combined detection of the three markers was

  9. Technical report on the fatigue crack Growth Benchmark based on CEA pipe bending tests

    International Nuclear Information System (INIS)

    In order to improve the estimation methods of surface crack propagation through the thickness of components, CEA has proposed a benchmark to members of the IAGE WG, sub-group on Integrity of metal components and structures. The subject is a simple configuration of a pipe containing an axisymmetric notch and submitted to a cyclic bending load. An experimental data-set form CEA was used to validate three issues in the topic of Leak Before Break. - Crack initiation, - Crack propagation through the thickness, - Crack penetration. All material and geometrical data which are necessary for the simulation were given in the proposal, including experimental results. Due to the peculiar complexity of the problem, it was decided to focus the work on methodologies comparison so as to allow participants to tune up parameters and adjust their models and tools. This report presents all estimations performed by the participants and collected by CEA. They are compared to the experimental results. An analysis of the used procedures is also proposed. This, associated with the study of the accuracy of different methodologies, leads to comments and recommendations on the analysis of fatigue crack growth. The participation in the first step was important: nine participants have proposed analyses, sometimes parametric analysis to estimate crack growth. Results sorted out three estimation methods groups that give results in accordance with experimental ones (these three groups are based on a strain range evaluation and the fatigue curve of the material): - The use of an elastic stress at the notch tip and a fatigue notch concentration factor to determine the strain range. - The use of a KI (or elastic F.E. calculation) and a Neuber rule for the estimation of the strain range at a characteristic distance from the crack tip. - The direct calculation of the strain range at the characteristic distance by an elastic plastic F.E. calculation. Only 4 participants have proposed an estimate of the

  10. Nuclear energy for the future: CEA strategic R and D orientations

    International Nuclear Information System (INIS)

    Worldwide energy consumption is expected to double over the next 50 years. If there is no major change in the trend, this growth should quickly cause depletion of fossil fuel resources, a dramatic increase in the contents of greenhouse effect gases and a serious supply risk for many countries. Beyond energy savings and the development of renewable energies, nuclear energy can provide a sustainable solution to these increased needs. This is the context in which the CEA is developing its nuclear R and D strategy for the future. One objective is to optimize and develop the current nuclear facilities : R and D for nuclear energy should increase the competitiveness of existing nuclear facilities, further improve safety. Water reactors (PWR and BWR), based on design principles that are now almost 40 years old, have been improved over time, but also made more complex due to increasing safety requirements, and more powerful through economic optimization. Enriched uranium fuels are safe and reliable. Pressurized water reactors are expected to become even more competitive in time with the new reactor designs (EPR) and the life extension of existing power stations. Nuclear energy sustainable development will only succeed with public acceptance. Solutions to Plutonium inventory management (see paper nr. 665) must be provided by either using it in fast neutron reactor where it as the most energy production efficiency or burning it out in existing - or future - PWR. Another objective is to bring efficient and acceptable solutions for HLLL waste management (see paper nr. 666) and reinforce knowledge on health and environment impact of nuclear activities. The third objective is to prepare and assess new generations of nuclear energy systems. Water reactors only consume 1% of the initial natural uranium and irradiated fuels still contain 96% of energetic material ; they also have a limited turbine efficiency. Industrial fast neutron reactors have been built. These reactors have

  11. [Antigenic response against PPD and antigen 60 in tubercular patients: single antigen versus the combined test].

    Science.gov (United States)

    Máttar, S; Broquetas, J M; Gea, J; Aran, X; el-Banna, N; Sauleda, J; Torres, J M

    1992-05-01

    We analyze serum samples from 70 patients with pulmonary tuberculosis and 50 healthy individuals. The antigenic activity (IgG) against protein purified antigen (PPD) and antigen 60 (A60) from M. tuberculosis. Thirteen patients were also HIV infected, and three patients had AIDS defined by the presence of disseminated tuberculosis. The test using antigen alone showed a 77% sensitivity and 74% specificity when PPD is used. When A60 was used, both values improved (81% sensitivity, 94% specificity). The use of a combined test (PPD and A60) improves the sensitivity (89%) but reduces the specificity (82%). The HIV infected patients showed similar responses to those of other patients. The combined use of different antigens might be useful for diagnosing tuberculosis. PMID:1390996

  12. Spanish pre-university students' use of English: CEA results from the University Entrance Examination

    Directory of Open Access Journals (Sweden)

    María Belén Díez Bedmar

    2011-12-01

    Full Text Available In this paper an updated overview of the main errors that Spanish students make when writing the English exam in the University Entrance Examination is provided. To do so, a Computer-aided Error Analysis (CEA (Dagneaux, Denness & Granger, 1998 was conducted on a representative sample of the students who took the exam in June 2008 in Jaén, and wrote a composition on the same topic. The use of the most widely-used error taxonomy, the Error Tagging Manual version 1.1. (Dagneaux, Denness, Granger & Meunier, 1996, and the analysis of the results by means of descriptive statistics foster the possibility to replicate this study and move forward in the description of the students’ written command in the foreign language at this stage. The comparison of the findings obtained in this study and those from previous (CEAs on the English exam reveals that some common tendencies may be shown.Este artículo aporta una visión general actualizada de los errores más importantes que los alumnos españoles cometen cuando escriben el examen de inglés de la Prueba de Acceso a la Universidad. Con este fin, se realizó un Análisis de Errores Informatizado (Dagneaux, Denness & Granger, 1998 en una muestra representativa de los estudiantes que realizaron el examen en Junio de 2008 en la Universidad de Jaén, y que escribieron sobre un mismo tema. El uso de la taxonomía de errores más usada, el Error Tagging Manual version 1.1. (Dagneaux, Denness, Granger & Meunier, 1996, y de la estadística descriptiva para analizar los resultados, favorecen la réplica de este estudio y la posibilidad de mejorar la descripción de la destreza escrita en la lengua extranjera. La comparación de los resultados en este artículo y otros Análisis de Errores (Informatizados previos muestran que se pueden exponer algunas tendencias comunes.

  13. 2004 annual report. Defense, safety, energy, information, health. CEA in the center of big European challenges; Rapport annuel 2004. Defense, securite, energie, information, sante. Le CEA au coeur des grands defis europeens

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    This document is the 2004 annual report of the French atomic energy commission (CEA). It presents the R and D activities of the CEA in three main domains: 1 - defense and safety, maintaining perenniality of nuclear dissuasion and nuclear safety: supplying nuclear weapons to armies, maintaining dissuasion capability with the simulation program, sharing R and D means with the scientific community and the industrial world, designing and maintaining naval nuclear propulsion reactors, cleansing Marcoule and Pierrelatte facilities, monitoring treaties and fighting against proliferation and terrorism; 2 - energy, developing more competitive and cleaner energy sources: nuclear waste management, optimization of industrial nuclear activities, future nuclear systems and new energy technologies, basic research on energy, radiobiology and toxicology; 3 - information and health, valorizing industry thanks to technological research and supplying new tools for health and medical research: micro- and nano-technologies, software technologies, basic research for industrial innovation, nuclear technologies for health and bio-technologies. (J.S.)

  14. Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells

    Directory of Open Access Journals (Sweden)

    Claudia Schlimper

    2012-01-01

    Full Text Available Adoptive therapy of malignant diseases with cytokine-induced killer (CIK cells showed promise in a number of trials; the activation of CIK cells from cancer patients towards their autologous cancer cells still needs to be improved. Here, we generated CIK cells ex vivo from blood lymphocytes of colorectal cancer patients and engineered those cells with a chimeric antigen receptor (CAR with an antibody-defined specificity for carcinoembryonic antigen (CEA. CIK cells thereby gained a new specificity as defined by the CAR and showed increase in activation towards CEA+ colon carcinoma cells, but less in presence of CEA− cells, indicated by increased secretion of proinflammatory cytokines. Redirected CIK activation was superior by CAR-mediated CD28-CD3ζ than CD3ζ signaling only. CAR-engineered CIK cells from colon carcinoma patients showed improved activation against their autologous, primary carcinoma cells from biopsies resulting in more efficient tumour cell lysis. We assume that adoptive therapy with CAR-modified CIK cells shows improved selectivity in targeting autologous tumour lesions.

  15. Nuclear systems of the future: international forum generation 4 and research and development projects at the Cea; Systemes nucleaires du futur: forum international generation 4 et projets de R et D du CEA

    Energy Technology Data Exchange (ETDEWEB)

    Carre, F

    2003-07-01

    To advance nuclear energy to meet future energy needs, ten countries have agreed to develop a future generation of nuclear energy systems, known as Generation 4. A technology road map to guide the Generation 4 effort was begun. This document presents the goals for these nuclear systems and the research programs of the Cea on the gas technology, GT-MHR, VHTR and GFR and the other systems as sodium Fast Neutron reactors, supercritical water and space nuclear. (A.L.B.)

  16. Electrochemical immunosensor based on nanoporpus gold loading thionine for carcinoembryonic antigen.

    Science.gov (United States)

    Sun, Xiaobin; Ma, Zhanfang

    2013-05-30

    Nanoporous gold (NPG) has recently received considerable attention in analytical electrochemistry because of its good conductivity and large specific surface area. A facile layer-by-layer assembly technique fabricated NPG was used to construct an electrochemical immunosensor for carcinoembryonic antigen (CEA). NPG was fabricated on glassy carbon (GC) electrode by alternatively assembling gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) using 1,4-benzenedimethanethiol as a cross-linker, and then AgNPs were dissolved with HNO3. The thionine was absorbed into the NPG and then gold nanostructure was electrodeposited on the surface through the electrochemical reduction of gold chloride tetrahydrate (HAuCl4). The anti-CEA was directly adsorbed on gold nanostructure fixed on the GC electrode. The linear range of the immunosensor was from 10 pg mL(-1) to 100 ng mL(-1) with a detection limit of 3 pg mL(-1) (S/N=3). The proposed immunosensor has high sensitivity, wide linear range, low detection limit, and good selectivity. The present method could be widely applied to construct other immunosensors.

  17. EURATOM-CEA association contributions to the 18. IAEA fusion energy conference

    International Nuclear Information System (INIS)

    The 9 contributions of EURATOM-Cea association to the fusion energy conference hold at Sorrento are gathered in this document with 7 additional papers. The different titles are: 1) Ergodic divertor experiments on the route to steady state operation of Tore-Supra, 2) High power lower hybrid current drive experiments in Tore-Supra tokamak, 3) Electron transport and improved confinement on Tore-Supra, 4) ECRH experiments and developments for long pulse in Tore-Supra, 5) Impurity penetration and contamination in Tore-Supra ergodic divertor experiments, 6) Real time plasma feed-back control: an overview of Tore-Supra achievements, 7) Numerical assessment of the ion turbulent thermal transport scaling laws, 8) Design of next step tokamak: consistent analysis of plasma flux consumption and poloidal, 9) Large superconducting conductors and joints for fusion magnets: from conceptual design to test at full size scale, 10) Burst-prone transport in tokamaks with internal transport barriers, 11) Electrostatic turbulence with finite parallel correlation length and radial electric field generation, 12) Theoretical issues in tokamak confinement: internal-edge transport barriers and runaway avalanche confinement, 13) Core and edge confinement studies with different heating methods in JET, 14) Confinement and transport studies of conventional scenarios in ASDEX upgrade, 15) First test results for the ITER central solenoid model coil, and 16) Progress of the ITER central solenoid model coil program

  18. METABOLISMO ÁCIDO DE LAS CRASULÁCEAS Crassulacean Acid Metabolism

    Directory of Open Access Journals (Sweden)

    THOMAS DAVID GEYDAN

    Full Text Available Se presenta una revisión del metabolismo ácido de las Crasuláceas, caracterizado por la ocurrencia, actividad y plasticidad del mecanismo desde un punto de vista fisiológico, bioquímico y molecular, enmarcado por la presencia de las denominadas cuatro fases de dicho metabolismo y su repercusión y expresión por diversas restricciones hídricas a nivel ecológico. Se presentan las principales enzimas y metabolitos básicos para el funcionamiento del metabolismo CAM, así como su modo de acción y control celular. Finalmente, se muestra que la plasticidad fenotípica en patrones de expresión CAM se encuentra mediada por condiciones ambientales y por señalizaciones moleculares.A review of Crassulacean acid metabolism is presented, characterized by showing the occurrence, activity and plasticity of these complex mechanism at the physiological, biochemical and molecular level, framed by the presence of the denominated four phases in CAM and its repercussion and expression due to different stresses in an ecological context. The basic enzymes, and metabolites necessary for the optional functioning of CAM are presented as well as their mode of action and cellular control. Finally, it is shown how environmental conditions and molecular signalling mediate the phenotypic plasticity.

  19. Enxertia herbácea em Myrtaceae nativas do Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Daiane Silva Lattuada

    2010-12-01

    Full Text Available O objetivo deste trabalho foi testar a viabilidade da técnica de enxertia herbácea em frutíferas nativas da família Myrtaceae. O experimento foi realizado em casa de vegetação do Departamento de Horticultura e Silvicultura/ UFRGS, em Porto Alegre. Duas espécies de Myrtaceae nativas foram usadas, tanto como porta-enxerto quanto como enxerto, Eugenia uniflora (Pitangueira e E. involucrata (Cerejeira-do-rio-grande ou Cerejeira-do-mato, totalizando quatro combinações. O método de enxertia adotado foi por garfagem em fenda cheia, em ramos herbáceos, com diâmetro médio do porta-enxerto de 0,1cm, enquanto os ramos dos enxertos apresentavam diâmetro médio entre 0,08 a 0,1cm, para as duas espécies. As análises foram quinzenais e, após 70 dias, foram analisadas estatisticamente a pega e a altura média das brotações emitidas. O delineamento experimental foi o completamente casualizado, com quatro repetições, sendo cada unidade experimental composta de cinco enxertos. Os resultados indicaram pega de 60% na combinação Pitangueira-Pitangueira (porta enxerto - enxerto. As demais combinações mostraram-se ineficientes.

  20. Meeting of Directors and Heads of Department from CEA-Saclay, France

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    Directors and Heads of Department from the Saclay centre of the French atomic energy commission (CEA) visited CERN in December. They are pictured here at the LHC magnet test facility in building SM18. On the left behind the visitors are the test benches where magnets are prepared for installation in String 2: the 120-metre full-scale model of an LHC cell of the regular part of the arc, which can be seen to the right. Photos 01, 02: Left to right: Marc Dejardin, Alain Givernaud, Louis Laurent, François Kircher, Antoine Masurel, Joël Feltesse, André Menez, T. Taylor, Philippe Pradelles, Bernard Sevestre, Michel Roche, Jean-Yves Gascoin, Thierry Lieven, Michel Spiro, Jean-Pierre Pervès, Yves Lemoigne, Béatrice Reder, Georges Carola, Philippe Lebrun, Yves Bourlat, Bertrand Mercier, Jean-Paul Langlois, Patrick Laurent, Didier Moulin, Xavier Vitart, Marc Serre, Jean-Louis Pierrey, Patrick Jarry, Bruno Mansoulié, Stéphane Lequien, Hervé Barbelin, Philippe Pallier, Jean-Yves Guillamot, and Norbert Sioegel.

  1. Report on transparency and nuclear safety - INB unexploited by the Marcoule CEA - 2012

    International Nuclear Information System (INIS)

    This report presents the different nuclear base installations (INB) of the Marcoule CEA centre, gives an overview of measures regarding safety within these installations (organisation, general arrangements, arrangements related to different risks, management of emergency situations, inspections, audits and second-level controls, arrangements and main events specific to the different installations and buildings) and of measures related to radiation protection (organisation and results, main events). It reports the significant events related to safety and radiation protection which occurred in 2012 and were declared to the ASN, and discusses how the return-on-experience has been used. It reports and comments the results of measurements of gaseous and liquid effluents, of their impact on the environment, and of surveys of the environment. It also presents the environmental management approach. The next part addresses the management of radioactive wastes which are warehoused on this site: arrangements aimed at limiting their volume, and at limiting their impact on health and on the environment, nature and quantities of warehoused wastes. Remarks and recommendations of the CHSCT are given

  2. Training and Certification Program for Certified Energy Auditors (CEA) and Certified Building Commissioning Professionals (CBCP)

    Energy Technology Data Exchange (ETDEWEB)

    Kent, Bill

    2012-08-24

    The Association of Energy Engineers (AEE) has offered energy efficiency training and certification programs for over 30 years. During that time AEE has certified more than 22,000 professionals. All of our certification programs are the result of extensive industry research and program development and oversight by certification boards. For this project award, AEE proposed to work with the Department of Energy to utilize and extend existing industry recognized Certified Energy Auditor (CEA) and Certified Building Commissioning Professional (CBCP) programs under this Training Program Development Announcement. These expanded training programs will have significant impact in training professionals for building commissioning and energy auditing to achieve the goal of bringing existing buildings up to their optimal energy performance potential and ensuring that new buildings maintain their expected optimal level of performance. The goals and objectives of the training development project were achieved with the development of new training programs that are now being offered as self-sustaining commercial training and certification programs. These new programs are training and certifying professionals who are accomplishing the goal of increasing building energy performance in both existing and new buildings.

  3. EURATOM-CEA association contributions to the 18. IAEA fusion energy conference

    Energy Technology Data Exchange (ETDEWEB)

    Ghendrih, Ph.; Peysson, Y.; Hoang, G.T. [and others

    2000-12-01

    The 9 contributions of EURATOM-Cea association to the fusion energy conference hold at Sorrento are gathered in this document with 7 additional papers. The different titles are: 1) Ergodic divertor experiments on the route to steady state operation of Tore-Supra, 2) High power lower hybrid current drive experiments in Tore-Supra tokamak, 3) Electron transport and improved confinement on Tore-Supra, 4) ECRH experiments and developments for long pulse in Tore-Supra, 5) Impurity penetration and contamination in Tore-Supra ergodic divertor experiments, 6) Real time plasma feed-back control: an overview of Tore-Supra achievements, 7) Numerical assessment of the ion turbulent thermal transport scaling laws, 8) Design of next step tokamak: consistent analysis of plasma flux consumption and poloidal, 9) Large superconducting conductors and joints for fusion magnets: from conceptual design to test at full size scale, 10) Burst-prone transport in tokamaks with internal transport barriers, 11) Electrostatic turbulence with finite parallel correlation length and radial electric field generation, 12) Theoretical issues in tokamak confinement: internal-edge transport barriers and runaway avalanche confinement, 13) Core and edge confinement studies with different heating methods in JET, 14) Confinement and transport studies of conventional scenarios in ASDEX upgrade, 15) First test results for the ITER central solenoid model coil, and 16) Progress of the ITER central solenoid model coil program.

  4. Estrutura da vegetação herbácea em paisagens ciliares no sul de Santa Catarina, Brasil

    OpenAIRE

    Aline Votri Guislon; Karoline Ceron; Guilherme Alves Elias; Robson Santos; Vanilde Citadini-Zanette

    2016-01-01

    As características estruturais e ecológicas da vegetação herbácea fazem com que ela seja sensível às alterações do ambiente, atuando como indicador da qualidade ambiental. No entanto, o estudo dessa comunidade ainda é incipiente em regiões neotropicais. Este estudo descreve a florística e os aspectos fitossociológicos da vegetação herbácea terrícola na mata ciliar de sete rios, no município de Urussanga, sul do estado de Santa Catarina, além de analisar e quantificar o status do conhecimento ...

  5. Larva migrans within scalp sebaceous gland Larva migrans em glândula sebácea do couro cabeludo

    Directory of Open Access Journals (Sweden)

    Lucinda Calheiros Guimarães

    1999-04-01

    Full Text Available A case of larva migrans or serpiginous linear dermatitis on the scalp of a teenager is reported. An ancylostomid larva was found within a sebaceous gland acinus. The unusual skin site for larva migrans as well as the penetration through the sebaceous gland are highlighted. The probable mechanism by which the parasite reached the skin adnexa is discussed.Relata-se caso de larva migrans ou dermatite linear serpiginosa no couro cabeludo de adolescente, no qual o ancilostomídeo foi encontrado no interior de glândula sebácea. Destaca-se a possibilidade do helminto sediar-se em locais pouco usuais, das glândulas sebáceas serem via de penetração de larvas na pele e discute-se o provável mecanismo pelo qual o agente implantou-se no anexo cutâneo.

  6. Prognostic Value of Pretreatment Carcinoembryonic Antigen After Definitive Radiotherapy With or Without Concurrent Chemotherapy for Squamous Cell Carcinoma of the Uterine Cervix

    International Nuclear Information System (INIS)

    Purpose: To evaluate whether pretreatment carcinoembryonic antigen (CEA) levels have a prognostic role in patients after definitive radiotherapy for squamous cell carcinoma (SCC) of the uterine cervix. Methods and Materials: A retrospective study of 550 patients was performed. The SCC antigen (SCC-Ag) and CEA levels were regarded as elevated when they were ≥2 and ≥5 ng/mL, respectively. A total of 208 patients underwent concurrent chemoradiotherapy (CCRT). The Kaplan-Meier method was used to calculate the distant metastasis (DM), local failure (LF), disease-free survival (DFS), and overall survival (OS) rates. Multivariate analysis was performed using the Cox proportional hazards model. The hazard ratio (HR) with 95% confidence interval (CI) was evaluated for the risk of a poor prognosis. Results: Compared with the patients with normal CEA/SCC-Ag levels, CEA levels ≥10 ng/mL but without elevated SCC-Ag levels was an independent factor for LF (HR, 51.81; 95% CI, 11.51–233.23; p < .001), DM (HR, 6.04; 95% CI, 1.58–23.01; p = .008), DFS (HR, 10.17; 95% CI, 3.18–32.56; p < .001), and OS (HR, 5.75; 95% CI, 1.82–18.18; p = .003) after RT alone. However, no significant role for CEA was noted in patients with SCC-Ag levels ≥2 ng/mL. In patients undergoing CCRT, a CEA level ≥10 ng/mL was an independent factor for LF (HR, 2.50; 95% CI, 1.01–6.21; p = .047), DM (HR, 3.41; 95% CI, 1.56–7.46; p = .002), DFS (HR, 2.73; 95% CI, 1.39–5.36; p = .003), and OS (HR, 3.93; 95% CI 1.99–7.75; p < .001). A SCC-Ag level of ≥40 ng/mL was another prognostic factor for DM, DFS, and OS in patients undergoing not only CCRT, but also RT alone. The 5-year OS rate for CCRT patients with CEA <10 ng/mL and ≥10 ng/mL was 75.3% and 35.8%, respectively (p < .001). CCRT was an independent factor for better OS (HR, 0.69; 95% CI, 0.50–0.97; p = .034). Conclusion: Pretreatment CEA levels in patients with SCC of the uterine cervix provide complementary information for

  7. Comparison of a new tumour marker CA 242 with CA 19-9, CA 50 and carcinoembryonic antigen (CEA) in digestive tract diseases.

    OpenAIRE

    Kuusela, P.; Haglund, C.; Roberts, P. J.

    1991-01-01

    The levels of CA 242, a new tumour marker of carbohydrate nature, were measured in sera of 185 patients with malignancies of the digestive tract and of 123 patients with benign digestive tract diseases. High percentages of elevated CA 242 levels (greater than 20 U ml-1) were recorded in patients with pancreatic and biliary cancers (68%). The sensitivity was somewhat lower than that of CA 19-9 (76%) and CA 50 (73%). On the other hand, in benign pancreatic and biliary tract diseases the CA 242 ...

  8. 一种新型免疫传感器阵列检测癌胚抗原和糖类抗原199技术%A New Type of Sensor Array Detection of Carcinoembryonic Antigen and Carbohydrate Antigen 199 Technology

    Institute of Scientific and Technical Information of China (English)

    周靖; 胡富陶; 余红卫

    2015-01-01

    制备了一种新型的含2个包覆分子印迹聚合物的丝网印刷碳工作电极的电化学发光夹心免疫传感器阵列,通过该阵列实现了肿瘤标志物癌胚抗原(CEA)和糖类抗原199(CA199)的近同时免疫分析。该新型的电化学发光免疫方法将空间分辨技术与分子印迹技术相结合,为 CEA 和CA199的复合免疫分析提供了一条简单、低成本、快速和灵敏的途径。该方法对临床实验中多种蛋白质的近同时测定也显示了较大的潜力。%In this study, a novel electrochemiluminescence (ECL) immunosensor array is engineered consisting of two screen-printed carbon working electrodes coated with molecular imprinting polymer (MIPs). The array is formed to perform multiplexed immunoassay of tumor markers (carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (CA199)). Based on spatial-resolved technique, and coupled with molecularly imprinted technique, this novel ECL strategy provides a simple, low-cost, fast and sensitive approach for multiplexed immunoassay of CEA and CA199. This approach may render promising potential for protein detection in the circumstances of clinical laboratory setting.

  9. Rosácea granulomatosa: relato de caso - enfoque terapêutico Granulomatous rosacea: case report - a therapeutic focus

    Directory of Open Access Journals (Sweden)

    Pedro Bezerra da Trindade Neto

    2006-10-01

    Full Text Available Os autores descrevem um caso de rosácea granulomatosa em um homem de 39 anos de idade, tratado com a associação de limeciclina oral e metronidazol gel tópico. A rosácea granulomatosa é uma variante da rosácea clássica, rara, caracterizada pela presença de pápulas vermelho-acastanhadas ou pequenos nódulos com base eritematosa e infiltrada, surgindo geralmente na superfície lateral da face e no pescoço. O exame histopatológico evidencia granulomas perifoliculares e perivasculares. A evolução é crônica, e o tratamento inclui antibióticos orais, como a tetraciclina e seus derivados, e medicações tópicas, como metronidazol, ácido retinóico, entre outras.The authors describe a case of granolumatous rosacea in a 39-year-old male, successfully treated with oral limecycline and topical gel of metronidazole. Granulomatous rosacea is a rare form of classic rosacea, characterized by brownish-red papules or small nodules on a diffusely reddened background and thickened skin. Lesions generally appear on the lateral surfaces of the face and on the neck. Histopathological examination shows perifollicular and perivascular granulomas. The course is chronic and treatment involves oral antibiotics, such as tetracycline and derivates; and topicals, such as metronidazole and topical retinoids.

  10. The use of tumour markers CEA, CA-195 and CA-242 in evaluating the response to chemotherapy in patients with advanced colorectal cancer.

    OpenAIRE

    Ward, U.; Primrose, J N; Finan, P. J.; Perren, T. J.; Selby, P.; Purves, D. A.; Cooper, E H

    1993-01-01

    Tumour markers CEA, CA-195 and CA-242 were measured in 33 patients undergoing chemotherapy for advanced colorectal cancer. The aim was to determine whether they could be used to accurately monitor the course of the disease, and reduce the need for imaging. Treatment with a 5-fluorouracil based regimen resulted in a partial response in nine patients (27%), whereas the remainder either had disease stabilisation or suffered from progression. Before treatment the CEA was elevated in 85% of patien...

  11. Role of tumor-associated antigen expression in radioimmunoguided surgery for colorectal and breast cancer.

    Science.gov (United States)

    Bertoglio, S; Percivale, P; Schenone, F; Peressini, A; Murolo, C; Badellino, F

    1998-12-01

    One hundred thirty-six patients with colorectal and breast cancer were enrolled in a retrospective study using radioimmunoguided surgery (RIGS) with Iodine-125 (I125) radiolabeled B72.3 (Group A, 73 patients) and F023C5 (Group B, 63 patients) monoclonal antibodies (MAbs). The correlation between intraoperative tumor-to-normal tissue (T/NT) gamma-detecting probe (GDP) counts ratio and the expression of tumor-associated glycoprotein (TAG)-72 (GroupA patients) and carcinoembryonic antigen (CEA; Group B patients) tumor-associated antigens (TAA) expression of 209 resected or biopsy tumor specimens was assessed. Ex vivo radioimmunolocalization index (R.I.) was carried out on the same specimens as a control of intraoperative GDP ratio values. RIGS positive definition of tumor occurred in 80/113 (70.8%) tumor sites of Group A patients and in 84/96 (87.5%) tumor sites of Group B patients. Mean percent B72.3 TAA expression of 113 tumor sites of Group A patients was 62.74 +/- 28.79% vs. 73.00 +/- 26.28% of 96 tumor sites of Group B patients (P < 0.05). The higher incidence of positive RIGS results was observed in tumor sites with the higher expression of the relative TAA. A statistically significant correlation between RIGS ratios and B72.3 and CEA expression was observed in the 113 tumor sites of Group A (P < 0.05) and in the 96 tumor sites of Group B (P < 0.01), respectively. The role of a preoperative evaluation of TAA expression in patients undergoing RIGS is discussed. Its assessment, whenever possible, may help to select those patients who will benefit more from this immunodiagnostic technique.

  12. Estrutura da vegetação herbácea em paisagens ciliares no sul de Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Aline Votri Guislon

    2016-06-01

    Full Text Available As características estruturais e ecológicas da vegetação herbácea fazem com que ela seja sensível às alterações do ambiente, atuando como indicador da qualidade ambiental. No entanto, o estudo dessa comunidade ainda é incipiente em regiões neotropicais. Este estudo descreve a florística e os aspectos fitossociológicos da vegetação herbácea terrícola na mata ciliar de sete rios, no município de Urussanga, sul do estado de Santa Catarina, além de analisar e quantificar o status do conhecimento científico relacionado às herbáceas terrícolas no Brasil. Foram levantadas 320 unidades amostrais de 4 m², nas quais todos os indivíduos foram identificados e dados relacionados aos parâmetros fitossociológicos foram coletados. A amostragem resultou em 58 táxons, 38 pertencentes às angiospermas e 20 às samambaias, distribuídos em 25 famílias botânicas. Poaceae apresentou maior riqueza, com 11 espécies. Estruturalmente, destacaram-se Goeppertia monophylla (Vell. Borchs. e S. Suárez, Blechnum brasiliense Desv. e Heliconia farinosa Raddi. A forma de vida mais frequente foi a hemicriptófita reptante. Os resultados revelam uma elevada riqueza de herbáceas terrícolas adaptadas às condições climáticas das matas ciliares, contribuindo para a diversidade da flora regional. Quanto à produção científica, foram encontrados 50 trabalhos indexados nas bases de dados eletrônicas (1990 a 2015, com destaque ao ano de 2011, que teve maior produção científica enfocando as herbáceas terrícolas. Embora com baixo número, a quantidade de indexações tende a crescer, em virtude do avanço da tecnologia de acesso às bases de dados e pela crescente adesão de periódicos em portais de busca.

  13. Produção integrada de Anonáceas no Brasil

    Directory of Open Access Journals (Sweden)

    Raimundo Braga Sobrinho

    2014-01-01

    Full Text Available Os conceitos técnicos e operacionais contidos na Produção Integrada (PI vêm atender de forma direta às exigências dos órgãos nacionais e internacionais que fiscalizam o comércio de "commodities", dando ênfase à segurança e a qualidade dos alimentos produzidos e consumidos pela população. A garantia da produção de um alimento seguro e rastreável é alcançada mediante o esforço harmônico de todos os integrantes da cadeia produtiva. Esse sistema pressupõe o cumprimento das Normas Técnicas Específicas (NTE para cada produto, permitindo o controle efetivo do sistema produtivo agropecuário por meio do monitoramento de todas as etapas na cadeia produtiva. Esse conceito teve início com o Manejo Integrado de Pragas (MIP e, posteriormente, expandiu-se para uma visão holística, estruturada em quatro pilares de sustentação: organização da base produtiva; sustentabilidade do sistema; monitoramento dos processos; e formação de um banco de dados. Dentro desta visão, insere-se a Produção Integrada de Anonáceas, projeto iniciado em 2010, com apoio do CNPq/MAPA/EMBRAPA e instituições parceiras. As anonáceas representam um nome genérico para designar as plantas da família Annonaceae constituída por cerca de 120 gêneros e em torno de 2.300 espécies. No Brasil, estão registrados 29 gêneros, dentro dos quais cerca de 260 espécies. Entre as espécies de maior importância comercial, destacam-se a graviola (Annona muricata L., pinha (Annona squamosa L., cherimólia (Annona cherimólia, Mill. e a atemoia, hibrido de A. cherimólia e A. squamosa. Essas frutas têm alta aceitação pelo seu sabor e possibilidade de uso para consumo in natura, sucos e geleias. As áreas comercialmente cultivadas são concentradas nos Estados do Nordeste do Brasil. Os Estados de maior concentração: Bahia para graviola e pinha, Alagoas para pinha e São Paulo para atemoia. Os resultados de pesquisa envolvendo toda a cadeia produtiva dessas

  14. Recent Improvements at CEA on Trace Analysis of Actinides in Environmental Samples

    International Nuclear Information System (INIS)

    In this paper, we present some results of R and D works conducted at CEA to improve on the one side the performance of the techniques already in use for detection of undeclared activities, and on the other side to develop new capabilities, either as alternative to the existing techniques or new methods that bring new information, complementary to the isotopic composition. For the trace analysis of plutonium in swipe samples by ICP-MS, we demonstrate that a thorough knowledge of the background in the actinide mass range is highly desirable. In order to avoid false plutonium detection in the femtogram range, correction from polyatomic interferences including mercury, lead or iridium atoms are in some case necessary. Efforts must be put on improving the purification procedure. Micro-Raman spectrometry allows determining the chemical composition of uranium compound at the scale of the microscopic object using a pre-location of the particles thanks to SEM and a relocation of these particles thanks to mathematical calculations. However, particles below 5 μm are hardly relocated and a coupling device between the SEM and the micro-Raman spectrometer for direct Raman analysis after location of a particle of interest is currently under testing. Lastly, laser ablation - ICP-MS is an interesting technique for direct isotopic or elemental analysis of various solid samples and proves to be a suitable alternative technique for particle analysis, although precision over isotopic ratio measurement is strongly limited by the short duration and irregularity of the signals. However, sensitivity and sample throughput are high and more developments are in progress to validate and improve this method. (author)

  15. Cytokeratins and carcinoembryonic antigen in diagnosis, staging and prognosis of colorectal adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Luís C. Fernandes; Su B. Kim; Delcio Matos

    2005-01-01

    AIM: To evaluate the serum levels of cytokeratins and carcinoembryonic antigen (CEA) in diagnosis, staging and prognosis of patients with colorectal adenocarcinoma.METHODS: The sample consisted of 169 patients. One hundred blood donors formed the control group. Radical surgery was performed on 120 patients, with an average follow-up duration of 22.3 mo. Relapses occurred in 23individuals after an average of 18.09 mo. CEA was assayed via the Delfia(R) method with a limit of 5 ng/mL. Cytokeratins were assayed via the LIA-mat(R) TPA-M Prolifigen(R) method with a limit of 72 U/L.RESULTS: In the diagnosis of patients with colorectal adenocarcinoma, CEA showed a sensitivity of 56%, a specificity of 95%, a positive predictive value of 94%, a negative predictive value of 50% and an accuracy of 76.8%.TPA-M had a sensitivity of 70%, a specificity of 96%, a positive predictive value of 97%, a negative predictive value of 66% and an accuracy of 93.6%. The elevation of one of the markers was shown to have a sensitivity of 76.9%, a specificity of 91%, a positive predictive value of 93.5%, a negative predictive value of 70% and an accuracy of 83.6%.There was no variation in the levels of the markers according to the degree of cell differentiation while there was an elevation in their concentrations in accordance with the increase in neoplastic dissemination. There was a statistically significant difference between the patients with stage Ⅳ lesions and those with stages Ⅰ, Ⅱ and Ⅲ tumors.With regard to CEA, the averagelevel was 14.2 ng/mL in patients with stage Ⅰ lesions, 8.5 ng/mL in patients with stage Ⅱ lesions, 8.0 ng/mL in patients with stage Ⅲ lesions and 87.7 ng/mL in patients with stage Ⅳ lesions. In relation to TPA-M, the levels were 153.1 U/L in patients with stage Ⅰtumors, 106.5 U/L in patients with stage Ⅱ tumors, 136.3 U/L in patients with stage Ⅲ tumors and 464.3 U/L in patients with stage Ⅳ tumors. There was a statistical difference in

  16. Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains.

    Science.gov (United States)

    Alvarez-Cienfuegos, Ana; Nuñez-Prado, Natalia; Compte, Marta; Cuesta, Angel M; Blanco-Toribio, Ana; Harwood, Seandean Lykke; Villate, Maider; Merino, Nekane; Bonet, Jaume; Navarro, Rocio; Muñoz-Briones, Clara; Sørensen, Karen Marie Juul; Mølgaard, Kasper; Oliva, Baldo; Sanz, Laura; Blanco, Francisco J; Alvarez-Vallina, Luis

    2016-01-01

    Here, we describe a new strategy that allows the rapid and efficient engineering of mono and multispecific trivalent antibodies. By fusing single-domain antibodies from camelid heavy-chain-only immunoglobulins (VHHs) to the N-terminus of a human collagen XVIII trimerization domain (TIE(XVIII)) we produced monospecific trimerbodies that were efficiently secreted as soluble functional proteins by mammalian cells. The purified VHH-TIE(XVIII) trimerbodies were trimeric in solution and exhibited excellent antigen binding capacity. Furthermore, by connecting with two additional glycine-serine-based linkers three VHH-TIE(XVIII) modules on a single polypeptide chain, we present an approach for the rational design of multispecific tandem trimerbodies with defined stoichiometry and controlled orientation. Using this technology we report here the construction and characterization of a tandem VHH-based trimerbody capable of simultaneously binding to three different antigens: carcinoembryonic antigen (CEA), epidermal growth factor receptor (EGFR) and green fluorescence protein (GFP). Multispecific tandem VHH-based trimerbodies were well expressed in mammalian cells, had good biophysical properties and were capable of simultaneously binding their targeted antigens. Importantly, these antibodies were very effective in inhibiting the proliferation of human epidermoid carcinoma A431 cells. Multispecific VHH-based trimerbodies are therefore ideal candidates for future applications in various therapeutic areas. PMID:27345490

  17. Actinides exposure: review of Ca-DTPA injections inside Cea-Cogema plants; Exposition aux actinides: bilan des injections de Ca-DTPA dans les centres CEA-Cogema

    Energy Technology Data Exchange (ETDEWEB)

    Grappin, L.; Berard, P.; Beau, P.; Carbone, L.; Castagnet, X.; Courtay, C.; Le Goff, J.P.; Menetrier, F.; Neron, M.; Piechowski, J. [CEA Cadarache, Dir. de l' Energie Nucleaire, Dept. de Soutien en surete et securite, Sev. de Sante au Travail, 13 - Saint-Paul-lez-Durance (France)

    2006-07-01

    Ca-DTPA has been used for medical treatment of plutonium and americium contaminations in the CEA and COGEMA plants from 1970 to 2003. This report is a survey of the injections administered of Ca-DTPA as a chelating molecule. This report will be a part of the AMM process for Ca-DTPA by intravenous administration submitted by the Central Pharmacy of the french Army. Out of 1158 injections administered to 469 persons, 548 events of possible or confirmed contaminations were reported. These employees were followed by occupational physicians according to the current regulations. The first part of the report is a synthesis of the most recent findings. Due to its short biological period and its limited action in the blood, Ca-DTPA does not chelate with plutonium and americium as soon as these elements are deposited in the target organs. It justifies an early treatment, even in cases of suspected contamination followed by additional injections if necessary. The second part presents data concerning these 1158 injections (way of contamination, posology, adverse effects...). These incidents took place at work, were most often minor, not requiring follow-up treatment. A study concerning the effectiveness of the product was done on a group of people having received 5 or more injections. These results were compared with effectiveness estimated from theoretical basis. Posologies and therapeutic schemes were proposed based on these observations. Additional studies are needed to confirm these findings. This document is the first synthesis in this field. It is the result of a collective work having mobilized the occupational medicine departments, the laboratories of CEA and COGEMA and a working group CEA-COGEMA-SPRA. (authors)

  18. [Optical Analysis of the Interaction of Mercaptan Derivatives of Nanogold Particles with Carcinoembryonic Antigen].

    Science.gov (United States)

    Zeng, Hong-juan; Zhao, Ran-lin; Wang, De-shun; Li, Cai-xia; Liu, Yi-yao

    2016-02-01

    be prepared. In this paper, novel mercaptan derivative of nanogold particles are prepared and studied using transmission electron microscopy (TEM), ultra-violet-visible absorption spectra (UV-Vis), fluorescence emission (FE) spectrum and infrared spectrum (IR) methods. The UV-Vis and FE results show the presence of new ligands mercaptan, more electrons from the orbit of ligand which can excite to the central ion related orbits and increase fluorescence of gold. Fluorescence sensitization effect was observed when mercaptan derivatives of nanogold interacted with carcinoembryonic antigen (CEA) and no fluorescence sensitization effect was found when nanogold interacted with carcinoembryonic antigen (CEA). The study of CEA hyperchromic mechanism of mercaptan derivatives nanogold and the CEA by the method of infrared spectrum, shows that the randomized OH bonds in the Au-protein interaction, showed more on the outside of the plane of bending vibration after the interaction with the mercaptan derivative nanogold, making the energy transfer from mercaptan derivatives nanogold to protein easy; leading to its fluorescence sensitization effect. PMID:27209753

  19. Evaluation of the radiology state at the CEA/Fontenay-aux-Roses and its environment; Point sur l'etat radiologique du CEA/Fontenay-aux-Roses et de son environnement

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-10-01

    Since 1946 the nuclear installations followed one other at the CEA of Fontenay-aux-Roses still their gradual stop the last ten years, except two installations INB34 and INB73, necessary for the wastes management. Today these installations form the subject of a drainage program. The public opinion is regularly informed on this program since 1999. This document presents the stock of the actions realized since this date: the track keeping of the sites activities impacts on the environment and the actions realized since 1999. (A.L.B.)

  20. Radioactivity and radioprotection: the every day life in a nuclear installation. Press tour at CEA/GRENOBLE 18 november 1999; Radioactivite et radioprotection: la vie quotidienne dans une installation nucleaire. Voyage de presse au Centre CEA/CADARACHE 18 novembre 1999

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-11-01

    In the framework of the public information, this paper gives a general information on the radioactivity and the radioprotection at the CEA/Cadarache center. A first part is devoted to a presentation of the radioactivity with definitions and radiation effects on the human being and the environment. An other part presents the radioprotection activities and regulations. The last part deals with specific activities of the CEA/Cadarache: the CASCAD installations for spent fuels storage, the LECA Laboratory for the Examination of Active Fuels and a dismantling installation for big irradiated objects. Historical aspects of the CEA/Cadarache are also provided. (A.L.B.)

  1. [Farmer's lung antigens in Germany].

    Science.gov (United States)

    Sennekamp, J; Joest, M; Sander, I; Engelhart, S; Raulf-Heimsoth, M

    2012-05-01

    Recent studies suggest that besides the long-known farmer's lung antigen sources Saccharopolyspora rectivirgula (Micropolyspora faeni), Thermoactinomyces vulgaris, and Aspergillus fumigatus, additionally the mold Absidia (Lichtheimia) corymbifera as well as the bacteria Erwinia herbicola (Pantoea agglomerans) and Streptomyces albus may cause farmer's lung in Germany. In this study the sera of 64 farmers with a suspicion of farmer's lung were examined for the following further antigens: Wallemia sebi, Cladosporium herbarum, Aspergillus versicolor, and Eurotium amstelodami. Our results indicate that these molds are not frequent causes of farmer's lung in Germany. PMID:22477566

  2. Positive predictive value of CEA and Ca19-9 as tumor markers for recurrent colorectal cancer in cases where conventional work-up fail to localize disease.

    Directory of Open Access Journals (Sweden)

    Yana Bocheva

    2015-12-01

    Full Text Available Introduction: Routine surveillance of colorectal cancer includes serial measurements of CEA levels. Although not routinely indicated Ca 19-9 is also a tool for recurrence. When any of these serum markers is elevated during follow up, this could represent a recurrence. The management of elevated tumor marker levels include clinical exams, endoscopy and conventional imaging –ultrasound, CT, MRI.Objective: To evaluate the positive predictive value of CEA and Ca19-9 as tumor markers for recurrent colorectal cancer in cases where conventional imaging and endoscopic studies fail to localize disease.Materials and methods: A total of 75 patients with elevated CEA and/or Ca19-9 serum levels and negative endoscopic exam as well as negative abdominal CT and Chest X-ray were included in the study. CEA levels were tested in 50 patients. Ca 19-9 was tested in 65 patients. 34 of the patients had both markers tested. All patients underwent whole body 18F-FDG PET/CT. Patients with negative of equivocal PET scan were further followed up (10 to 24 months.Results: Based on the reference standard – the results from PET/CT, if positive and the results from follow-up in cases of negative or equivocal scans, the positive predictive value of Ca 19-9 was 84% and that of CEA -83%. There was no significant difference in the PPV of Ca19-9 and CEA.Conclusion: Elevated CEA and Ca 19-9 levels in patients under active surveillance after operation for colorectal cancer have high positive predictive value for recurrence, even in cases where conventional work-up – endoscopy and CT don’t localize disease.

  3. Relationship between peripheral and mesenteric serum levels of CEA and CA 242 with staging and histopathological variables in colorectal adenocarcinoma Níveis séricos periféricos e mesentéricos de CEA e CA 242, estadiamento e variáveis histopatológicas no adenocarcinoma colorretal

    Directory of Open Access Journals (Sweden)

    Mauro Lamelas Cardoso

    2009-10-01

    Full Text Available PURPOSE: To compare histopathological variables and staging in colorectal adenocarcinoma cases with CEA and CA 242 in peripheral and mesenteric blood. METHODS: In 169 individuals underwent surgery for colorectal cancer, CEA and CA 242 were analyzed and compared to mesenteric and peripheral blood and correlated with macroscopic tumor's morphology and size, degree of cell differentiation, venous, neural and lymphatic involvement and TNM classification. RESULTS: There was a difference between the mesenteric (M and peripheral (P serum levels of CEA (p=0.020. Higher levels of markers were correlated with venous invasion CEA (P p=0.013, CEA (M p=0.05, CA 242 (M p=0.005 and CA 242 (P p=0.038; with advanced staging CEA (P OBJETIVO: Comparar variáveis histopatológicas e graus de estadiamento do adenocarcinoma colorretal com níveis sanguíneos periféricos e mesentéricos de CEA e CA-242. MÉTODOS: Em 169 doentes submetidos ao tratamento cirúrgico por adenocarcinoma colorretal, CEA e CA-242 foram analisados e comparados quanto aos níveis sanguíneos periféricos e mesentéricos e correlacionados com o tamanho e a morfologia macroscópica do tumor, grau de diferenciação celular, invasões venosa, linfática, neural e a classificação TNM. RESULTADOS: Verificou-se diferença significante entre o nível sérico mesentérico e periférico de CEA (p= 0,02. Níveis séricos mais elevados dos marcadores foram observados e correlacionados com invasão venosa, CEA (P p=0,013, CEA(M, p=0,05, CA-242 (M p=0,005 e CA-242 (P p=0,038. Grau de estadiamento TNM avançado foi associado com CEA(P < CEA(M p<0,05, CA-242(P < CA-242(M p<0,05. Nas maiores dimensões tumorais constatou-se CEA(P < CEA(M p=0,001 e CA 242 (P < CA 242 (M (p < 0.001. O CA 242 periférico e mesentérico aumentados associaram-se com a invasão neural, p=0.014 e p=0.003, respectivamente. CONCLUSÕES: O nível sérico mesentérico de CEA é superior ao nível sérico periférico. Os níveis s

  4. LAS GESNERIÁCEAS DEL DEPARTAMENTO DEL QUINDÍO, COLOMBIA

    Directory of Open Access Journals (Sweden)

    Oscar Humberto Marín-Goméz

    2014-12-01

    Full Text Available Colombia es el país neotropical con mayor diversidad de Gesneriaceae. Sin embargo hay vacíos en el conocimiento de los patrones de distribución y ecología de esta familia, debido a su complejidad taxonómica. En este trabajo se presenta una lista de Gesneriaceae para el departamento del Quindío con datos de hábitat y distribución altitudinal. Se revisaron las colecciones del COL y HUQ y se realizó trabajo de campo entre 2009 y 2014 en 70 localidades del Quindío. Se encontraron 43 especies y 11 géneros, con una mayor representación de Columnea, Besleria y Kohleria; nueve especies fueron nuevos registros para el Quindío, tres fueron endémicas y cinco especies nuevas para la ciencia. La mayor riqueza se encontró en los bosques montanos entre 1800 m y 2400 m, principalmente en interior bosque y cañadas. Los resultados de este trabajo constituyen una línea base para dirigir la investigación en ecología, conservación y su potencial ornamental de las gesneriáceas presentes en el Quindío.ABSTRACTColombia is the most diverse Neotropical country of Gesneriaceae. Nevertheless, there are gaps in knowledge of the distribution patterns and ecology of this family because of their taxonomic complexity. A list of the Gesneriaceae of the Quindío department with data for habitat and altitudinal distribution is presented in this paper. The herbarium collections of COL and HUQ were reviewed and fieldwork was carried out between 2009 and 2014 in 70 localities of Quindío. Forty three species and 11 genera were found, with a larger representation of Columnea, Besleria and Kohleria genera; nine species were new records for the Quindío; three were endemic and five species are new to science. The greatest richness was found in the montane forest between 1800 m and 2400 m, mainly in forested areas and streams. The results of this work constitute a baseline to conduct research in ecology, conservation and ornamental potential of Gesneriaceae

  5. Sandia National Laboratories results for the 2010 criticality accident dosimetry exercise, at the CALIBAN reactor, CEA Valduc France.

    Energy Technology Data Exchange (ETDEWEB)

    Ward, Dann C.

    2011-09-01

    This document describes the personal nuclear accident dosimeter (PNAD) used by Sandia National Laboratories (SNL) and presents PNAD dosimetry results obtained during the Nuclear Accident Dosimeter Intercomparison Study held 20-23 September, 2010, at CEA Valduc, France. SNL PNADs were exposed in two separate irradiations from the CALIBAN reactor. Biases for reported neutron doses ranged from -15% to +0.4% with an average bias of -7.7%. PNADs were also exposed on the back side of phantoms to assess orientation effects.

  6. Fractionated 131I anti-CEA radioimmunotherapy: effects on xenograft tumour growth and haematological toxicity in mice

    OpenAIRE

    Violet, J A; Dearling, J L J; Green, A. J.; Begent, R H J; Pedley, R B

    2008-01-01

    Dose fractionation has been proposed as a method to improve the therapeutic ratio of radioimmunotherapy (RIT). This study compared a single administration of 7.4 MBq 131I-anti-CEA antibody given on day 1 with the same total activity given as fractionated treatment: 3.7 MBq (days 1 and 3), 2.4 MBq (days 1, 3, and 5) or 1.8 MBq (days 1, 3, 5, and 8). Studies in nude mice, bearing the human colorectal xenograft LS174T, showed that increasing the fractionation significantly reduced the efficacy o...

  7. CA 242, a new tumour marker for pancreatic cancer: a comparison with CA 19-9, CA 50 and CEA.

    OpenAIRE

    Haglund, C.; Lundin, J; Kuusela, P.; Roberts, P. J.

    1994-01-01

    The serum expression of a novel tumour marker, CA 242, defined by monoclonal antibody C 242, was studied in 179 patients with pancreatic cancer. The results were compared with CA 19-9, CA 50 and CEA. CA 242 is a carbohydrate closely related, but not identical, to CA 19-9 and CA 50. The overall sensitivity of the CA 242 assay was 74%: 55% in stage I, 83% in stage II-III and 78% in stage IV disease. The specificity calculated from 112 patients with benign diseases was 91%. CA 19-9 had a higher ...

  8. Rapport d'étude : La perception des risques majeurs par les riverains du CEA de Cadarache

    OpenAIRE

    Lopez, Alexia; Régner, Isabelle; Schleyer-Lindenmann, Alexandra

    2015-01-01

    Ce rapport d'étude vient répondre aux interrogations de son commanditaire, la CLI (Commission Local d'Information) du CEA (Commissariat à l'énergie Atomique) de Cadarache. Il s'organise en trois partie : une première partie établit un état des lieux des connaissances des risques majeurs par les habitants. Les risques majeurs officiellement recensés sont : le risque feu de forêt, le risque inondation, le risque sismique, le risque de mouvement de terrain, le risque de rupture de barrage, et le...

  9. Studies of monoclonal antibodies IOR-CEA-1 and IOR-EGF/R3 labelled with 99mTc

    International Nuclear Information System (INIS)

    Nuclear Medicine is a speciality that uses radioisotopes for the diagnosis or treatment of diseases and it is considered one of the best tools among the diagnostic modalities for detection of cancer. 99mTc is one of the main isotopes for labelling antibodies and in Nuclear Medicine in general, due to its adequate physical properties, availability and low cost. Labelled monoclonal antibodies have shown promising results for diagnosis and therapy of cancer and their use has brought great experimental and clinical advances in the field of oncology. The main clinical applications of immunoscintigraphy with monoclonal antibodies are staging and evaluation of tumoral reappearance. The antibodies employed in this work were: OIR-CEA-1, a murine monoclonal antibody that acts directly against CEA expressed in several neoplasia in particular those from the gastrointestinal tract (colorectal cancer) and IOR-EGF/R3, a murine monoclonal antibody that binds to the external domain of EGF-R and it has been used in the diagnosis of tumors of epithelial origin. The objectives of this work were the development and optimization of the reduction and purification processes, the radiolabelling techniques and quality control procedures (radiochemical, immunoreactivity and cystein challenge) and imaging studies of monoclonal antibodies OIR-CEA-1 and IOR-EGF/R3, using the simple, fast and efficient method of direct labelling of the antibody with 99mTc. The final results was the definition of the best conditions for the preparation of lyophilized reactive kits of OIR-CEA-1 and IOR- EGF/R3 for an efficient diagnostic application in Nuclear Medicine. The most adequate conditions for the labelling of the antibodies were: 1.0 mg Ab, 29 μL MDP, 3.0 μg Sn2+, 1 mL of 99mTc and 30 min. reaction time. With these conditions the labelling yield was always higher than 95% and the maximum activity of 99mTc was about 2220 MBq (60 mCi). The evidences of the efficiency and quality of the methods here

  10. Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride.

    Science.gov (United States)

    Wang, Huan; Wang, Yaoguang; Zhang, Yong; Wang, Qi; Ren, Xiang; Wu, Dan; Wei, Qin

    2016-06-06

    Carcinoembryonic antigen (CEA) was used as the model, an ultrasensitive label-free photoelectrochemical immunosensor was developed using 2D TiO2 nanosheets and carboxylated graphitic carbon nitride (g-C3N4) as photoactive materials and ascorbic acid as an efficient electron donor. 2D TiO2 nanosheets was sythsized by surfactant self-assembly method and proved to have higher photoelectrochemical signals than TiO2 nanoparticles. Firstly, carboxylated g-C3N4 could be attached to 2D TiO2 nanosheets through the bond formed between carboxyl group of carboxylated g-C3N4 and TiO2. And the photocurrent of g-C3N4/TiO2 drastically enhances compared to carboxylated g-C3N4 and TiO2. Then, antibody of CEA was bonded to TiO2 through the dentate bond formed between carboxyl group of anti-CEA and TiO2, leading to the decrease of the photocurrents. As proven by PEC experiments and electrochemical impedance spectroscopy (EIS) analysis, the fabrication process of the immunosensor is successful. Under the optimal conditions, the intensity decreased linearly with CEA concentration in the range of 0.01~10 ng/mL. The detection limit is 2.1 pg/mL. The work provides an effective method for the detection of tumor markers and can be extended for the application in food safety and environmental monitoring analysis.

  11. Natural selection promotes antigenic evolvability

    NARCIS (Netherlands)

    Graves, C.J.; Ros, V.I.D.; Stevenson, B.; Sniegowski, P.D.; Brisson, D.

    2013-01-01

    The hypothesis that evolvability - the capacity to evolve by natural selection - is itself the object of natural selection is highly intriguing but remains controversial due in large part to a paucity of direct experimental evidence. The antigenic variation mechanisms of microbial pathogens provide

  12. Oncogenic cancer/testis antigens

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Andersen, Mads H; Ditzel, Henrik J

    2015-01-01

    /testis antigens are immunogenic, highly cancer-specific, and frequently expressed in various types of cancer, which make them promising candidate targets for cancer immunotherapy, including cancer vaccination and adoptive T-cell transfer with chimeric T-cell receptors. Our current understanding of tumor...

  13. The importance of preoperative elevated serum levels of CEA and CA15-3 in patients with breast cancer in predicting its histological type.

    Directory of Open Access Journals (Sweden)

    J Słonina

    2010-06-01

    Full Text Available It is not known whether in patients with breast cancer the occurrence of elevated serum tumour markers depends on its histological type. The aim of the study was to assess relationship between breast cancer histological type and the presence of increased serum levels of CEA and CA 15-3. The study population was 428 patients (all women, mean age 52.5 years, treated at The Department of Surgery of Wroclaw Medical University from 2005 to 2008 due to breast cancer. All of them had their preoperative CA 15-3 and CEA serum concentrations measured. According to the TNM system, 21% of patients were in stage I, 32.5% in stage II, 46.5% in stage III of the disease. In patients with ductal type of the cancer the elevated serum levels of CEA and CA 15-3 were observed in 48.7% and 42.2%, in lobular type in 42.4% and 52.5%, and in non-ductal/tubular types in 48.1% and 40.4% (p=N/S. Stepwise logistic regression analyses showed that ductal breast cancer is related to elevated CEA and normal CA 15-3 serum levels. The histological types of breast cancer are not significantly related to elevated serum levels of CEA and/or CA 15-3.

  14. The clinical significance of preoperative serum CEA,β-HCG and CXs detection in the diagnosis of non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    Xian-Hua Yang; Juan Chen

    2016-01-01

    Objective:To study the clinical significance of preoperative serum CEA,β-HCG and CXs detection in the diagnosis of non-small cell lung cancer and provide reference for clinical diagnosis and disease treatment.Methods:Non-small cell lung cancer patients treated in our hospital from April 2009 to April 2014 were analyzed. Correlation between preoperative serum CEA,β-HCG as well as Cxs and clinical stages as well as prognosis of non-small cell lung cancer was assayed. Healthy subjects in our hospital during the same period were taken as control group.Results:Serum CEA,β-HCG and Cxs had no obvious correlation with patients’ age and gender, but CEA andβ-HCG had negative correlation with the clinical stages and prognosis of non-small cell lung cancer while Connexin43 had positive correlation with the clinical stages and prognosis of non-small cell lung cancer.Conclusions:Preoperative serum CEA combined withβ-HCG and CXs detection can be taken as key molecules in clinical diagnosis and prognosis of non-small cell lung cancer.

  15. Concepts and applications for influenza antigenic cartography

    Science.gov (United States)

    Cai, Zhipeng; Zhang, Tong; Wan, Xiu-Feng

    2011-01-01

    Influenza antigenic cartography projects influenza antigens into a two or three dimensional map based on immunological datasets, such as hemagglutination inhibition and microneutralization assays. A robust antigenic cartography can facilitate influenza vaccine strain selection since the antigenic map can simplify data interpretation through intuitive antigenic map. However, antigenic cartography construction is not trivial due to the challenging features embedded in the immunological data, such as data incompleteness, high noises, and low reactors. To overcome these challenges, we developed a computational method, temporal Matrix Completion-Multidimensional Scaling (MC-MDS), by adapting the low rank MC concept from the movie recommendation system in Netflix and the MDS method from geographic cartography construction. The application on H3N2 and 2009 pandemic H1N1 influenza A viruses demonstrates that temporal MC-MDS is effective and efficient in constructing influenza antigenic cartography. The web sever is available at http://sysbio.cvm.msstate.edu/AntigenMap. PMID:21761589

  16. Genome Scale Identification of Treponema pallidum Antigens

    OpenAIRE

    McKevitt, Matthew; Brinkman, Mary Beth; McLoughlin, Melanie; Perez, Carla; Howell, Jerrilyn K.; Weinstock, George M.; Norris, Steven J; Palzkill, Timothy

    2005-01-01

    Antibody responses for 882 of the 1,039 proteins in the proteome of Treponema pallidum were examined. Sera collected from infected rabbits were used to systematically identify 106 antigenic proteins, including 22 previously identified antigens and 84 novel antigens. Additionally, sera collected from rabbits throughout the course of infection demonstrated a progression in the breadth and intensity of humoral immunoreactivity against a representative panel of T. pallidum antigens.

  17. Relationship between peripheral and mesenteric serum levels of CEA and CA 242 with staging and histopathological variables in colorectal adenocarcinoma Níveis séricos periféricos e mesentéricos de CEA e CA 242, estadiamento e variáveis histopatológicas no adenocarcinoma colorretal

    OpenAIRE

    Mauro Lamelas Cardoso; Luís Cesar Fernandes; Su Bong Kim; Delcio Matos

    2009-01-01

    PURPOSE: To compare histopathological variables and staging in colorectal adenocarcinoma cases with CEA and CA 242 in peripheral and mesenteric blood. METHODS: In 169 individuals underwent surgery for colorectal cancer, CEA and CA 242 were analyzed and compared to mesenteric and peripheral blood and correlated with macroscopic tumor's morphology and size, degree of cell differentiation, venous, neural and lymphatic involvement and TNM classification. RESULTS: There was a difference between th...

  18. Neutron Activation Foil and Thermoluminescent Dosimeter Responses to a Polyethylene Reflected Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [French Atomic Energy Commission (CEA), Saclay (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Authier, Nicolas [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Piot, Jerome [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Jacquet, Xavier [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Rousseau, Guillaume [French Atomic Energy Commission (CEA), Salives (France). Valduc Centre for Nuclear Studies; Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 19, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. The CIDAS detects gammas with a Geiger-Muller tube and the Rocky Flats detects neutrons via charged particles produced in a thin 6LiF disc depositing energy in a Si solid state detector. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  19. Neutron Activation Foil and Thermoluminescent Dosimeter Responses to a Lead Reflected Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Isbell, Kimberly McMahan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Gagnier, Emmanuel [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Authier, Nicolas [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Piot, Jerome [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Jacquet, Xavier [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Rousseau, Guillaume [Commissariat a l' Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France); Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 13, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. The CIDAS detects gammas with a Geiger-Muller tube, and the Rocky Flats detects neutrons via charged particles produced in a thin 6LiF disc, depositing energy in a Si solid-state detector. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  20. Neutron Activation and Thermoluminescent Detector Responses to a Bare Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [ORNL; Isbell, Kimberly McMahan [ORNL; Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette; Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Piot, Jerome [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille; Reynolds, Kevin H. [Y-12 National Security Complex

    2016-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 11, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  1. Neutron Activation and Thermoluminescent Detector Responses to a Bare Pulse of the CEA Valduc SILENE Critical Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Piot, Jerome [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Reynolds, Kevin H. [Oak Ridge Y-12 Plant (Y-12), Oak Ridge, TN (United States)

    2015-09-01

    This benchmark experiment was conducted as a joint venture between the US Department of Energy (DOE) and the French Commissariat à l'Energie Atomique (CEA). Staff at the Oak Ridge National Laboratory (ORNL) in the US and the Centre de Valduc in France planned this experiment. The experiment was conducted on October 11, 2010 in the SILENE critical assembly facility at Valduc. Several other organizations contributed to this experiment and the subsequent evaluation, including CEA Saclay, Lawrence Livermore National Laboratory (LLNL), the Y-12 National Security Complex (NSC), Babcock International Group in the United Kingdom, and Los Alamos National Laboratory (LANL). The goal of this experiment was to measure neutron activation and thermoluminescent dosimeter (TLD) doses from a source similar to a fissile solution critical excursion. The resulting benchmark can be used for validation of computer codes and nuclear data libraries as required when performing analysis of criticality accident alarm systems (CAASs). A secondary goal of this experiment was to qualitatively test performance of two CAAS detectors similar to those currently and formerly in use in some US DOE facilities. The detectors tested were the CIDAS MkX and the Rocky Flats NCD-91. These detectors were being evaluated to determine whether they would alarm, so they were not expected to generate benchmark quality data.

  2. Diagnostic value of CYFRA 21-1 and CEA for predicting lymph node metastasis in operable lung cancer.

    Science.gov (United States)

    Chen, Feng; Yan, Cui-E; Li, Jia; Han, Xiao-Hong; Wang, Hai; Qi, Jun

    2015-01-01

    Tumour markers are used extensively for the management of lung cancer, including diagnosis, evaluating effectiveness of treatments, monitoring recurrence after therapy and for predicting prognosis. However, there exists a knowledge gap regarding potential quantitative correlations between tumour marker levels and the extents of lymph node involvement in primary lung cancer. The current study is comprised of 139 lung cancer patients scheduled to undergo surgical operation. Of the 139 patients, 107 were subsequently diagnosed with lung cancer without lymph node involvement and 32 were diagnosed with malignant disease with lymph node involvement by histological examination. Preoperative tumour marker levels were quantified in each patient. The median tumour marker levels were statistically higher in lung cancer patients with malignant lymph nodes than in those who suffered either benign lung disease or carcinoma in situ (Kruskal-Wallistest; P = 0.001). Tumour marker levels were significantly correlated with clinical stage (ANOVA; P = 0.009). When examined as a dichotomous variable (CYFRA 21-1 ≤ 5.0 and CEA ≤ 5.0 group and CYFRA 21-1 > 5.0 or CEA > 5.0 group), elevated tumour marker levels correlated strongly with the presence of positive lymph nodes (χ(2) test; P = 0.000). This correlation suggests that the tumour marker levels are clinical predictors for the malignant involvement of lymph nodes in operable lung cancer patients. PMID:26309663

  3. Low dose radiation effects: an integrative european approach (Risc-Rad Project) coordinated by the Cea

    International Nuclear Information System (INIS)

    RISC-RAD (Radiosensitivity of Individuals and Susceptibility to Cancer induced by ionizing Radiations) is an Integrated Project funded by the European Commission under 6. Framework Programme / EURATOM. RISC-RAD started on 1. January 2004 for a duration of four years. Coordinated by Cea (Dr Laure Sabatier), it involves 11 European countries (Austria, Denmark, Finland, France, Germany, Ireland, Italy, the Netherlands, Spain, Sweden and the United Kingdom) and 29 research institutions. Objectives: Exposures to low and protracted doses of ionizing radiation are very frequent in normal living environment, at work places, in industry and in medicine. Effects of these exposures on human health cannot be reliably assessed by epidemiological methods, nor is thoroughly understood by biologists. RISC-RAD project proposes to help bridging the gap of scientific knowledge about these effects. To achieve this goal, a necessary key step is to understand the basic mechanisms by which radiation induces cancer. Studying this multistage process in an integrated way, the project offers a new biological approach characterised by and clear-cut and objective-driven scientific policy: the project is focused on the effects of low doses (less than 100 mSv) and protracted doses of radiation. It aims at identifying new parameters that take into account the differences in radiation responses between individuals. A group of modelers works closely with the experimental teams in order to better quantify the risks associated with low and protracted doses. Research work is divided into five work packages interacting closely with each other. WP1 is dedicated to DNA damage. Ionizing Radiation (IR) produce a broad spectrum of base modifications and DNA strand breaks of different kinds, among which double-strand breaks and 'clustered damage' which is thought to be a major feature in biological effectiveness of IR. The aim of Work Package 1 is to improve understanding of the initial DNA damage induced by

  4. Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine

    Institute of Scientific and Technical Information of China (English)

    YUAN Ruo; ZHUO Ying; CHAI YaQin; ZHANG Ying; SUN AiLi

    2007-01-01

    Electrochemical sensing of carcinoembryonic antigen (CEA) on a gold electrode modified by the sequential incorporation of the mediator, thionine (Thi), and gold nanoparticles (nano-Au), through covalent linkage and electrostatic interactions onto a self-assembled monolayer configuration is described in this paper. The enzyme, horseradish peroxidase (HRP), was employed to block the possible remaining active sites of the nano-Au monolayer, avoid the non-specific adsorption, instead of bovine serum albumin (BSA), and amplify the response of the antigen-antibody reaction. Electrochemical experiments indicated highly efficient electron transfer by the imbedded Thi mediator and adsorbed nano-Au. The HRP kept its activity after immobilization, and the studied electrode showed sensitive response to CEA and high stability during a long period of storage. The working range for the system was 2.5 to 80.0 ng/mL with a detection limit of 0.90 ng/mL. The model membrane system in this work is a potential biosensor for mimicking the other immunosensor and enzyme sensor.

  5. Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Electrochemical sensing of carcinoembryonic antigen(CEA)on a gold electrode modified by the se- quential incorporation of the mediator,thionine(Thi),and gold nanoparticles(nano-Au),through co- valent linkage and electrostatic interactions onto a self-assembled monolayer configuration is de- scribed in this paper.The enzyme,horseradish peroxidase(HRP),was employed to block the possible remaining active sites of the nano-Au monolayer,avoid the non-specific adsorption,instead of bovine serum albumin(BSA),and amplify the response of the antigen-antibody reaction.Electrochemical ex- periments indicated highly efficient electron transfer by the imbedded Thi mediator and adsorbed nano-Au.The HRP kept its activity after immobilization,and the studied electrode showed sensitive response to CEA and high stability during a long period of storage.The working range for the system was 2.5 to 80.0 ng/mL with a detection limit of 0.90 ng/mL.The model membrane system in this work is a potential biosensor for mimicking the other immunosensor and enzyme sensor.

  6. Evaluating the value of CEA and CA153 during follow-up in HER-2 overexpressing breast cancer u-sing ROC curves%利用 ROC 曲线评价 CEA、CA153在 HER -2阳性乳腺癌术后随访中的价值

    Institute of Scientific and Technical Information of China (English)

    侯军君; 任立瑾; 高媛媛; 刘潇莲; 蔡莉

    2014-01-01

    目的:用ROC曲线评价CEA及CA153监测HER-2阳性乳腺癌患者术后复发转移的价值,并确立诊断HER-2阳性乳腺癌患者术后复发转移的最佳临界值。方法收集127例HER-2阳性乳腺癌患者CEA及CA153数值,将其分为复发转移及未复发转移两组。绘制ROC曲线,找到最佳临界值,同时比较最佳临界值与常规诊断值诊断复发转移的优劣。结果复发转移组较未复发转移组CEA及CA153水平显著偏高(P<0.0001),CEA、CA153诊断复发转移ROC曲线下面积分别为0.752、0.820,对应的最佳临界值分别为3.5 ng/mL、17.89 U/mL。 CEA和CA153最佳临界值敏感性分别为46.15%、65.38%,CEA和CA153最佳临界值特异性分别为97.33%、89.33%。结论 CEA、CA153在HER-2阳性乳腺癌患者术后判断复发转移中具有中等价值,诊断术后复发转移的最佳临界值分别为3.5 ng/mL、17.89 U/mL。%Objective To explore the value of carcinoembryonic antigen ( CEA) and cancer antigen 153 ( CA153 ) in detecting tumor recurrences and to establish the optimal operating point of diagnosis of recurrences in HER-2 overexpressing breast cancer using ROC curves .Methods A total of 127 HER -2 overexpressing breast cancer patients was enrolled into this study and subdivided into two groups ,the recurrence group and the non-recurrence group .We created a ROC curve and found out the optimal operating point .Then we compared the advantage of detecting recurrences between optimal operating point in the ROC curve and the diagnostic point that we usually used .Results The recurrence groups showed significantly higher CEA and CA 153 levels than the non-recurrence groups(P<0.0001).The area under the curve(AUC)of the CEA and CA153 were 0.752 and 0.820 respectively and the optimal operating point were 3.5 ng/mL and 17.89 U/mL,respectively.The sensitivi-ties of the optimal operating point of CEA and CA 153 were 46.15%and 65.38%respectively

  7. Clinical significance of combined determination of several tumor markers (including CYFRA21-1, NSE, CA-50 and CEA) in patients with pulmonary cancer

    International Nuclear Information System (INIS)

    Objective: To enhance the diagnosis of pulmonary cancer by determination of optimal combinations of various tumor markers. Methods: Serum CYFRA21-1, NSE, CA-50 (with RIA) and CEA (with CLIA) contents were determined in 107 patients with various types of pulmonary cancers, 66 patients with various benign pulmonary diseases and 59 controls. Results: It was revealed that CYFRA21-1 determination was most sensitive for detection of squamous cell carcinoma. The same was true for CEA in the detection of adenocarcinoma. NSE determination was very specific for small cell carcinoma. Combined determinations of either CYFRA21-l + NSE or CYFRA21-1 + NSE + CEA were excellent for general screening. Conclusion: Combined determination of these tumor markers could be applied expediently as supplementary diagnostic measure for pulmonary malignancies. (authors)

  8. Antigen Export Reduces Antigen Presentation and Limits T Cell Control of M. tuberculosis.

    Science.gov (United States)

    Srivastava, Smita; Grace, Patricia S; Ernst, Joel D

    2016-01-13

    Persistence of Mycobacterium tuberculosis results from bacterial strategies that manipulate host adaptive immune responses. Infected dendritic cells (DCs) transport M. tuberculosis to local lymph nodes but activate CD4 T cells poorly, suggesting bacterial manipulation of antigen presentation. However, M. tuberculosis antigens are also exported from infected DCs and taken up and presented by uninfected DCs, possibly overcoming this blockade of antigen presentation by infected cells. Here we show that the first stage of this antigen transfer, antigen export, benefits M. tuberculosis by diverting bacterial proteins from the antigen presentation pathway. Kinesin-2 is required for antigen export and depletion of this microtubule-based motor increases activation of antigen-specific CD4 T cells by infected cells and improves control of intracellular infection. Thus, although antigen transfer enables presentation by bystander cells, it does not compensate for reduced antigen presentation by infected cells and represents a bacterial strategy for CD4 T cell evasion.

  9. Ultrasensitive non-enzymatic immunosensor for carcino-embryonic antigen based on palladium hybrid vanadium pentoxide/multiwalled carbon nanotubes.

    Science.gov (United States)

    Han, Jian; Jiang, Liping; Li, Faying; Wang, Ping; Liu, Qing; Dong, Yunhui; Li, Yueyun; Wei, Qin

    2016-03-15

    A novel and sensitive sandwich-type non-enzymatic electrochemical immunosensor was fabricated for quantitative monitoring of carcino-embryonic antigen (CEA). Nanocomposite of stannic oxide/reduced graphene oxide was used as substrate material to increase the specific surface area and enhance the conductivity of the glassy carbon electrode. Gold nanoparticles (Au NPs) were introduced to link substrate materials and primary antibodies (Ab1) and accelerate the electron transfer in this system. At the same time, the palladium nanoparticles (Pd NPs)-vanadium pentoxide (V2O5)/multiwalled carbon nanotubes (MWCNTs) were used as the label of secondary antibodies (Ab2). This composite label has shown excellent catalytic activity towards the reduction of H2O2. The nanomaterial-based signal amplification can improve the sensitivity and lower the limit of detection. The proposed immunosensor showed wide linear range from 0.5 pgmL(-1) to 25 ngmL(-1) with limit of detection of 0.17 pgmL(-1). This novel immunosensor was used to analyze serum sample. The results indicated that this immunosensor may find huge potential application for quantitative detection of CEA in the clinical diagnosis. PMID:26562331

  10. Diagnostic performance of CD66c in lung adenocarcinoma-associated malignant pleural effusion: comparison with CEA, CA 19-9, and CYFRA 21-1.

    Science.gov (United States)

    Son, Seung-Myoung; Han, Hye-Suk; An, Jin Young; Choe, Kang Hyeon; Lee, Ki Man; Lee, Ki Hyeong; Kim, So-Seul; Lee, Yong-Moon; Lee, Ho-Chang; Song, Hyung Geun; Lee, Ok-Jun

    2015-02-01

    Various tumour markers have been evaluated in malignant pleural effusions, but not CD66c. This study evaluated the diagnostic ability of CD66c in lung adenocarcinoma-associated malignant pleural effusions (LA-MPEs) and compared it with other known tumour markers. Forty-seven cases of LA-MPE and 52 cases of benign pleural effusions were collected. The levels of CD66c, CEA, CA 19-9, and CYFRA 21-1 were measured by enzyme immunoassay. The expression of CD66c, CEA, and CA 19-9 in cell blocks was measured by immunocytochemistry. CEA had the best diagnostic values, with a sensitivity of 87.2% and specificity of 92.3%. Both CD66c and CA 19-9 showed the highest specificity of 98.1%, with sensitivities of 63.8% and 55.3%, respectively. CYFRA 21-1 had a sensitivity of 83.0% and specificity of 76.9%. CEA combined with CA 19-9 reached a sensitivity of 91.5% and a specificity of 98.1%. The sensitivities of immunocytochemical staining for CD66c, CEA, and CA 19-9 were 72.5%, 75%, and 40%, respectively. CD66c showed a diagnostic performance comparable to CYFRA 21-1 and CA 19-9 by enzyme immunoassay. Immunocytochemical study showed that CD66c and CEA were more sensitive than CA19-9. Both studies support CD66c as a potential tumour marker to differentiate LA-MPE from benign effusions. PMID:25551300

  11. Antigenic Variation in Plasmodium falciparum.

    Science.gov (United States)

    Petter, Michaela; Duffy, Michael F

    2015-01-01

    Plasmodium falciparum is the protozoan parasite that causes most malaria-associated morbidity and mortality in humans with over 500,000 deaths annually. The disease symptoms are associated with repeated cycles of invasion and asexual multiplication inside red blood cells of the parasite. Partial, non-sterile immunity to P. falciparum malaria develops only after repeated infections and continuous exposure. The successful evasion of the human immune system relies on the large repertoire of antigenically diverse parasite proteins displayed on the red blood cell surface and on the merozoite membrane where they are exposed to the human immune system. Expression switching of these polymorphic proteins between asexual parasite generations provides an efficient mechanism to adapt to the changing environment in the host and to maintain chronic infection. This chapter discusses antigenic diversity and variation in the malaria parasite and our current understanding of the molecular mechanisms that direct the expression of these proteins. PMID:26537377

  12. [HLA antigens in juvenile rheumatoid arthritis].

    Science.gov (United States)

    Rumba, I V; Sochnev, A M; Kukaĭne, E M; Burshteĭn, A M; Benevolenskaia, L I

    1990-01-01

    Antigens of I class HLA system (locus A and B) were investigated in 67 patients of Latvian nationality suffering from juvenile rheumatoid arthritis (JRA). Associations of HLA antigens with juvenile rheumatoid arthritis partially coincided with the ones revealed earlier. Typing established an increased incidence of antigen B27 (p less than 0.01) and gaplotype A2, B40 (p less than 0.01). Antigen B15 possessed a protective action with respect to JRA. Interlocus combinations demonstrated a closer association with the disease than a single antigen. The authors also revealed markers of various clinico-anatomical variants of JRA.

  13. 联合检测血清CEA、CYFRA21-1、NSE在肺癌诊断、分期、分型中的意义%Clinical Values of Combined Serum CEA,CYFRA21-1 and NSE Detection in Diagnosis,Staging and Predicting Pathologic Typing of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    徐恩赐; 王利利; 周菊英

    2012-01-01

    目的 探讨血清癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)在肺癌诊断、临床分期以及病理分型中的作用.方法 采用化学发光法、放射免疫分析法和酶联免疫吸附法分别检测114 例肺癌患者和89例肺部良性疾病患者血清CEA、CYFRA21-1、NSE水平.结果 肺癌组血清CEA、CYFRA21-1、NSE含量明显高于肺良性疾病组(P<0.05);CEA、CYFRA21-1及NSE诊断肺癌的ROC曲线下面积分别是0.60、0.85、0.82;联合检测对肺癌诊断的灵敏度明显高于任何单一检测(P<0.05);Ⅰ+Ⅱ期肺癌组血清CEA含量明显高于肺良性疾病组(P<0.05);CEA 在腺癌组含量明显高于鳞癌组(P<0.05);NSE在小细胞肺癌组含量明显高于鳞癌组和腺癌组(P<0.05);血清CEA在Ⅰ、Ⅱ、Ⅲ期非小细胞肺癌组含量明显低于Ⅳ期(P<0.05);CYFRA21-1在Ⅰ、Ⅱ期非小细胞肺癌组含量明显低于Ⅲ、Ⅳ期(P<0.05).结论 CEA对肺癌的早期诊断有一定意义;血清CEA对腺癌的灵敏度最佳,NSE对小细胞肺癌的灵敏度最佳;早期肺癌血清CEA、CYFRA21-1水平明显低于晚期肺癌;三种肿瘤标志物联合检测可明显提高肺癌诊断的敏感性,对肺癌的临床诊断、病理分型、临床分期具有重要的临床意义.%Objective To evaluate the value of combined serum carcinoembryonic antigen(CEA),cytokeratin fragment 19(CYFRA21-1) and neuron-specific enolase(NSE) detection for diagnosis,staging and predicting of pathologic types in patients with pulmonary carcinoma.Methods The levels of CEA,CYFRA21-1 and NSE were measured in 114 patients with pulmonary carcinoma and 89 patients with pulmonary benign lesion,using chemoluminescence,RIA and ELISA methods respectively.Results The concentrations of CEA,CYFRA21-1 and NSE in the cancer patients group were obviously higher than those in benign lung diseases group(P < 0.05). In single index the ROC area of serum CEA,CYFRA21-1 and NSE was 0

  14. Las cactáceas del Valle de Tehuacán-Cuicatlán, México: estimación de la calidad del muestreo

    OpenAIRE

    César Miguel-Talonia; Oswaldo Téllez-Valdés; Miguel Murguía-Romero

    2014-01-01

    El Valle de Tehuacán-Cuicatlán (VTC) es conocido, entre otros motivos, por la riqueza y endemismo de cactáceas que alberga, pero ¿cómo se distribuye esta familia en el VTC, los registros disponibles de éstas son representativos de su distribución geográfica? Los objetivos del presente trabajo son describir la distribución espacial de las especies de cactáceas en el VTC y estimar la calidad del muestreo. Con base en la incidencia de las especies dentro de unidades geográficas operativas (UGO) ...

  15. Stable solid-phase Rh antigen.

    Science.gov (United States)

    Yared, M A; Moise, K J; Rodkey, L S

    1997-12-01

    Numerous investigators have attempted to isolate the Rh antigens in a stable, immunologically reactive form since the discovery of the Rh system over 56 years ago. We report here a successful and reproducible approach to solubilizing and adsorbing the human Rh antigen(s) to a solid-phase matrix in an antigenically active form. Similar results were obtained with rabbit A/D/F red blood cell antigens. The antigen preparation was made by dissolution of the red blood cell membrane lipid followed by fragmentation of the residual cytoskeleton in an EDTA solution at low ionic strength. The antigenic activity of the soluble preparations was labile in standard buffers but was stable in zwitterionic buffers for extended periods of time. Further studies showed that the antigenic activity of these preparations was enhanced, as was their affinity for plastic surfaces, in the presence of acidic zwitterionic buffers. Adherence to plastic surfaces at low pH maintained antigenic reactivity and specificity for antibody was retained. The data show that this approach yields a stable form of antigenically active human Rh D antigen that could be used in a red blood cell-free assay for quantitative analysis of Rh D antibody and for Rh D antibody immunoadsorption and purification.

  16. The significance of MT, VEGF, CA125, CEA and MMP-9 combine detection in NSCLC diagnosis%联合检测外周血CEA、CA125、VEGF、MT及MMP-9在晚期非小细胞肺癌诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    丁瑞敏

    2015-01-01

    目的:探讨外周血金属硫蛋白(metallothionein, MT)、血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)、糖链抗原125(carbohydrate antigen 125, CA125)、癌胚抗原(carcinoem-bryonic antigen, CEA)和基质金属蛋白酶-9(matrix metalloprotein-9, MMP-9)5项指标联合检测对晚期非小细胞肺癌(non-small-cell lung carcinoma, NSCLC)的诊断价值。方法选择2012年6月至2014年6月于我院就诊的NSCLC 患者80例及健康对照者72例为研究对象,检测受试者血清 MT、VEGF、CA125、CEA和MMP-9水平,对检测结果进行统计学分析。结果 NSCLC组MT、VEGF、CA125、CEA和MMP-9的检测结果均高于对照组,且差异均有统计学意义(P均<0.05)。5项指标联合检测的灵敏度和特异性均高于各指标单独检测的灵敏度和特异性。结论 MT、VEGF、CA125、CEA和MMP-9的检测可用于晚期NSCLC的辅助诊断,其联合检测可明显提高NSCLC的检出率。%Objective To analysis the significance of peripheral blood metallothionein(MT), vascular endothelial growth factor(VEGF), carbohydrate antigen 125(CA125), carcinoembryonic antigen(CEA), and matrix metalloprotein-9 (MMP-9) combine detection in advanced stage non-small-cell lung carcinoma (NSCLC) diagnosis. Methods 80 cases patients with advanced stage NSCLC and 72 cases healthy control people from June 2012 to June 2014 in our hospital were collected. The MT, VEGF, CA125, CEA and MMP-9 serum levels of all subjects were all detected and the results were analyzed statistically. Results The serum levels of MT, VEGF, CA125, CEA and MMP-9 in NSCLC group were all higher than that of control group, and the differences all had statistical significance (Pall< 0.05). The sensitivity and specificity of 5 index combine detection were all higher than that of each index alone detection. Conclusion The detection of MT, VEGF, CA125, CEA and MMP-9 can be used in the

  17. INIS - International Nuclear Information System. CEA-users relations. Nuclear information in total freedom; INIS - International Nuclear Information System. Relations CEA utilisateurs. L'information nucleaire en toute liberte

    Energy Technology Data Exchange (ETDEWEB)

    Surmont, J.; Paillard, M.D.; Constant, A.; Guille, N.; Le Blanc, A.; Mouffron, O.; Anguise, P.; Jouve, J.J.; Lilin, A

    2009-07-01

    This poster, prepared for the seventh edition of the meetings of scientific and technical information professionals (RPIST, Nancy (France)), presents the principle of operation of the INIS system, based on a cooperative and decentralized preparation of the data which are centralized by the IAEA for the preparation of the INIS products: the database (on Internet and on CD/DVD-Rom), the collection of grey literature documents in full text (microfiches and CD/DVD-roms), and the multilingual thesaurus available in 7 different languages and under various formats. The poster presents these different products, and the role of the INIS members in the distribution of these products towards their national users. One insert presents the French INIS team of the CEA-Saclay and its daily work. (J.S.)

  18. Utility of endoscopic ultrasound, cytology and fluid carcinoembryonic antigen and CA 19-9 levels in pancreatic cystic lesions

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To assess the diagnostic accuracy of endoscopic ultrasound (EUS), fluid tumor markers and cytology in distinguishing benign from (pre)malignant pancreatic cystic lesions.METHODS: 46 consecutive patients, referred to a gastroenterologist and surgeon for a symptomatic or incidental pancreatic cyst, were reviewed. EUS, cytology,and carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19-9) levels were compared with the final diagnosis, based on surgical pathology and/or imaging follow-up of at least 12 mo. Cysts were classified as benign (pseudocyst, serous cystadenoma) or malignant/pre-malignant (mucinous cystic neoplasm). Receiveroperator characteristics (ROC) curve analysis was performed.RESULTS: The mean age was 56 years; 29% were male and median cyst diameter was 3 cm. Final outcome was obtained in 41 (89%) patients. Twenty-three (56%) of these 41 had surgical pathology. Twenty-three (56%)had benign lesions and 18 (44%) had malignant/premalignant lesions. Sensitivity, specificity and positive and negative predictive value of EUS alone to distinguish benign from malignant/premalignant pancreatic cystic lesions were 50%, 56%, 36% and 54% and for cytology were 71%, 96%, 92% and 85%, respectively. The corresponding values for the ROC-derived ideal cutoffs were 75%, 90%, 75%, 90% for CA 19-9 (> 37 U/mL)and 70%, 85%, 79% and 78% for CEA (> 3.1 ng/mL).Subgroup analysis of those with surgical pathology yielded almost identical performance and cutoffs.CONCLUSION: Cytology and cyst fluid tumor marker analysis is a very useful tool in distinguishing benign from (pre)malignant pancreatic cystic lesions.

  19. Origin and evolution of cultivated cucurbits Origem e evolução de cucurbitáceas cultivadas

    Directory of Open Access Journals (Sweden)

    Dilson Antônio Bisognin

    2002-08-01

    Full Text Available Cucurbits (Cucurbitaceae are among the most important plant families supplying humans with edible products and useful fibers. Plants of this family are very similar in above ground development, but they have high genetic diversity for fruit shape and other fruit characteristics, resulting in a variety of uses. The objective of this review was to discuss the origin and evolution of the most important cultivated cucurbits. Understanding the evolutionary history and domestication process increase the possibility for better exploiting the genetic diversity for cultivar development. The domestication selection in cucurbits was for shape, less bitter flesh, larger and fewer seeds, and larger fruit size, resulting in high genetic diversity within and among cultivated species. This variation can be associated with the wide range of uses that require different shape, size and a constant ratio between fruit length and fruit diameter. The discussion of the breeding history indicates how artificial selection could speed up changes in fruit characteristics to attend specific uses and increase adaptation to a variety of environmental conditions in which cucurbits are growing worldwide. Although interspecific hybridization has been employed in cucurbit breeding more than in any other family, there is still a high potential for increasing its application for germplasm and cultivar development.As cucurbitáceas (Cucurbitaceae são uma das mais importantes famílias de plantas utilizadas para produção de alimentos e fibras. Apesar de a parte aérea das plantas desta família ser muito similar em seu desenvolvimento, grande variabilidade genética tem sido mantida para formato e outras características de fruto, o que aumenta o seu potencial de uso. O objetivo desta revisão foi discutir a origem e a evolução das cucurbitáceas cultivadas. A história da evolução e o conhecimento das alterações ocorridas durante este processo podem facilitar a utiliza

  20. Overview of CEA's R&D on GFR fuel element design: from challenges to solutions

    International Nuclear Information System (INIS)

    Over the period 2002-2012, CEA conducted some extensive R&D on the design of GFR fuel elements (together with related material and core/system studies). This paper reviews the challenges raised by this programme, the solutions proposed to address them, and the remaining issues. Studies were performed on the assembly duct, the pin bundle and the fuel pin. The main issues were related to the challenge of using silicon carbide composites (SiC/SiC) for the pin cladding and the assembly duct, as well as mixed uranium-plutonium carbide (UPuC) for the nuclear fuel. Emphasizing the pin design, key achievements are reviewed in this paper regarding such topics as fission product confinement and high burnup performance, for the sake of which original design options were recently patented. (author)

  1. Terahertz Real-Time Imaging Uncooled Arrays Based on Antenna-Coupled Bolometers or FET Developed at CEA-Leti

    Science.gov (United States)

    Simoens, François; Meilhan, Jérôme; Nicolas, Jean-Alain

    2015-10-01

    Sensitive and large-format terahertz focal plane arrays (FPAs) integrated in compact and hand-held cameras that deliver real-time terahertz (THz) imaging are required for many application fields, such as non-destructive testing (NDT), security, quality control of food, and agricultural products industry. Two technologies of uncooled THz arrays that are being studied at CEA-Leti, i.e., bolometer and complementary metal oxide semiconductor (CMOS) field effect transistors (FET), are able to meet these requirements. This paper reminds the followed technological approaches and focuses on the latest modeling and performance analysis. The capabilities of application of these arrays to NDT and security are then demonstrated with experimental tests. In particular, high technological maturity of the THz bolometer camera is illustrated with fast scanning of large field of view of opaque scenes achieved in a complete body scanner prototype.

  2. List of reports in the field of reactor safety research sponsored by BMFT, CEA, EPRI, JSTA and USNRC

    International Nuclear Information System (INIS)

    The list reviews reports from the Federal Republic of Germany, from France, from Japan and from the United States of America concerning single problems in the field of reactor safety research. According to the cooperation of the Federal Minister for Research and Technology (BMFT) with the Commissariat a l'Energie Atomique (CEA), the Japan Science and Technology Agency (JSTA), the Electric Power Research Institute (EPRI) and the United States Nuclear Regulatory Commission, these reports are available in the Gesellschaft fuer Reaktorsicherheit (GRS). The list pursues the following order: Country of origin, problem area concerned, according to the Reactor Safety Research Programme of the BMFT, reporting organization. The list of reports appears quarterly. (orig.) With 142 refs

  3. From basic science to social issue: the CEA's role in nuclear energy and alternative sources of energy

    International Nuclear Information System (INIS)

    By force of circumstance, thoroughgoing changes are under way in the ways we consume and produce energy. Research and development must respond to these changes by making technological innovations and proposing solutions that are safe, competitive, economic with regard to natural resources and respectful of the environment. The French ''Commissariat a l'Energie Atomique et aux energies alternatives'' (CEA) has a leading role to play through its large range of activities: fundamental research and technological research in various fields from biology to nuclear sciences via energy efficiency of buildings and solar energy and through its access to big experimental facilities like Orphee and Osiris reactors or peta-watt Lasers or Soleil synchrotron

  4. List of reports of BMFT, CEA, EPRI, JSTA and USNRC in the field of reactor safety research

    International Nuclear Information System (INIS)

    This list reviews reports from the Federal Republic of Germany, from France, from Japan and from the United States of America concerning special problems in the field of Reactor Safety Research. According to the cooperation of the Bundesminister fuer Forschung und Technologie (BMFT) with the Commisariat a l'Energie Atomique (CEA), the Japan Science and Technology Agency (JSTA), the Electrical Power Research Institute (EPRI) and the United States Nuclear Regulatory Commision, these reports are available in the Gesellschaft fuer Reaktorsicherheit (GRS). The list pursues the following order: Country of origin, problem area concerned, according to the Reactor Safety Research Programm of the BMFT, reporting organization. The list of reports appears quaterly. (orig./HP)

  5. Improvements on Low Level Activity Gamma Measurements and X-ray Spectrometry at the CEA-MADERE Measurement Platform

    Directory of Open Access Journals (Sweden)

    Sergeyeva Victoria

    2016-01-01

    Full Text Available The CEA MADERE platform (Measurement Applied to DosimEtry in REactors is a part of the Instrumentation Sensors and Dosimetry Laboratory (LDCI. This facility is dedicated to the specific activity measurements of solid and radioactive samples using Gamma and X-ray spectrometry. MADERE is a high-performance facility devoted to neutron dosimetry for experimental programs performed in CEA and for the irradiation surveillance programmes of PWR vessels. The MADERE platform is engaged in a continuous improvement process. Recently, two High Efficiency diodes have been integrated to the MADERE platform in order to manage the accurate low level activity measurements (few Bq per sample. This new equipment provides a good level of efficiency over the energy range from 60 keV to 2 MeV. The background continuum is reduced due to the use of a Ultra Low Background (ULB lead shielding. Relative and absolute X-ray measurement techniques have been improved in order to facilitate absolute rhodium activity measurement (Rh103m on solid samples. Additional efforts have been made to increase the accuracy of the relative niobium (Nb93m activity measurement technique. The way of setting up an absolute measurement method for niobium is under investigation. After a presentation of the MADERE's measurement devices, this paper focuses on the technological options taken into account for the design of high efficiency measurement devices. Then, studies performed on X-ray measurement techniques are presented. Some details about the calculation of uncertainties and correction factors are also mentioned. Finally, future research and development axes are exposed.

  6. Concordance of Hypermethylated DNA and the Tumor Markers CA 15-3, CEA, and TPA in Serum during Monitoring of Patients with Advanced Breast Cancer

    DEFF Research Database (Denmark)

    Kristiansen, Søren; Jørgensen, Lars Mønster; Høgh Hansen, Morten;

    2015-01-01

    The serological protein tumor markers CA 15-3, CEA, and TPA are frequently used to monitor tumor burden among metastatic breast cancer patients. Breast cancer is associated with global DNA hypomethylation and hypermethylation of some promoter regions. No monitoring study has yet investigated the ...

  7. Antigen Incorporation on Cryptosporidium parvum Oocyst Walls

    OpenAIRE

    Entrala Emilio; Sbihi Younes; Sánchez-Moreno Manuel; Mascaró Carmen

    2001-01-01

    Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidi...

  8. Histocompatibility antigens in coal miners with pneumoconiosis.

    OpenAIRE

    Soutar, C A; Coutts, I.; Parkes, W R; Dodi, I. A.; Gauld, S; Castro, J E; Turner-Warwick, M

    1983-01-01

    Twenty-five histocompatibility antigens have been measured in 100 coal miners with pneumoconiosis attending a pneumoconiosis medical panel and the results compared with a panel of 200 normal volunteers not exposed to dust. Chest radiographs were read independently by three readers according to the ILO U/C classification. On a combined score, 40 men were thought to have simple pneumoconiosis and 60 men complicated pneumoconiosis. The number of antigens tested and associations between antigens ...

  9. A computational framework for influenza antigenic cartography.

    Directory of Open Access Journals (Sweden)

    Zhipeng Cai

    Full Text Available Influenza viruses have been responsible for large losses of lives around the world and continue to present a great public health challenge. Antigenic characterization based on hemagglutination inhibition (HI assay is one of the routine procedures for influenza vaccine strain selection. However, HI assay is only a crude experiment reflecting the antigenic correlations among testing antigens (viruses and reference antisera (antibodies. Moreover, antigenic characterization is usually based on more than one HI dataset. The combination of multiple datasets results in an incomplete HI matrix with many unobserved entries. This paper proposes a new computational framework for constructing an influenza antigenic cartography from this incomplete matrix, which we refer to as Matrix Completion-Multidimensional Scaling (MC-MDS. In this approach, we first reconstruct the HI matrices with viruses and antibodies using low-rank matrix completion, and then generate the two-dimensional antigenic cartography using multidimensional scaling. Moreover, for influenza HI tables with herd immunity effect (such as those from Human influenza viruses, we propose a temporal model to reduce the inherent temporal bias of HI tables caused by herd immunity. By applying our method in HI datasets containing H3N2 influenza A viruses isolated from 1968 to 2003, we identified eleven clusters of antigenic variants, representing all major antigenic drift events in these 36 years. Our results showed that both the completed HI matrix and the antigenic cartography obtained via MC-MDS are useful in identifying influenza antigenic variants and thus can be used to facilitate influenza vaccine strain selection. The webserver is available at http://sysbio.cvm.msstate.edu/AntigenMap.

  10. A computational framework for influenza antigenic cartography.

    Science.gov (United States)

    Cai, Zhipeng; Zhang, Tong; Wan, Xiu-Feng

    2010-10-07

    Influenza viruses have been responsible for large losses of lives around the world and continue to present a great public health challenge. Antigenic characterization based on hemagglutination inhibition (HI) assay is one of the routine procedures for influenza vaccine strain selection. However, HI assay is only a crude experiment reflecting the antigenic correlations among testing antigens (viruses) and reference antisera (antibodies). Moreover, antigenic characterization is usually based on more than one HI dataset. The combination of multiple datasets results in an incomplete HI matrix with many unobserved entries. This paper proposes a new computational framework for constructing an influenza antigenic cartography from this incomplete matrix, which we refer to as Matrix Completion-Multidimensional Scaling (MC-MDS). In this approach, we first reconstruct the HI matrices with viruses and antibodies using low-rank matrix completion, and then generate the two-dimensional antigenic cartography using multidimensional scaling. Moreover, for influenza HI tables with herd immunity effect (such as those from Human influenza viruses), we propose a temporal model to reduce the inherent temporal bias of HI tables caused by herd immunity. By applying our method in HI datasets containing H3N2 influenza A viruses isolated from 1968 to 2003, we identified eleven clusters of antigenic variants, representing all major antigenic drift events in these 36 years. Our results showed that both the completed HI matrix and the antigenic cartography obtained via MC-MDS are useful in identifying influenza antigenic variants and thus can be used to facilitate influenza vaccine strain selection. The webserver is available at http://sysbio.cvm.msstate.edu/AntigenMap.

  11. Establishment of Immunoradiometric Assay for Carcinoembryonic Antigen

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Two anti-CEA monoclonal antibodies are used, one is coated on the microtiter plate, the other is labeled to make 125I-CEAMcAb. The one-step assay is established based on immunoradiometric assay(IRMA). The sensitivity of the assay is 0.5 μ g/L. The intra-assay CVs and the inter-assay CVs are lower than 10.0% and 15.0%, respectively. The analytical recoveries are ranged from 97.4% to 107.8%. The reference cut-out value of 35 normal serum is lower than

  12. The value of combined detection of serum SCCA,CYFRA21-1 and CEA in diagnosis of lung cancer%血清SCCA、CYFRA21-1、CEA联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    王小芳; 罗玲

    2013-01-01

    目的 评价血清鳞状细胞癌抗原(SCCA)、细胞角蛋白片段21-1(CYFRA21-1)、癌胚抗原(CEA)联合检测在肺癌诊断中的价值.方法 选择重庆市肿瘤研究所2008年1~4月住院患者共63例,收集所有患者血清检测SCCA、CYFRA21-1及CEA.其中肺癌44例,肺部良性病变者19例,肺癌患者中小细胞肺癌5例、鳞癌17例、腺癌15例,未定型肺癌7例,所有肺癌患者均为病理确诊.结果 肺癌组SCCA、CYFRA21-1、CEA水平均高于肺部良性病变组(P<0.01),在不同病理类型中,3种指标升高的程度不等,SCCA 及CYFRA21-1水平在鳞癌组最高,CEA水平在腺癌组最高,与其他组比较差异有统计学意义(P<0.01).结论 血清SCCA、CYFRA21-1、CEA检测在肺癌的诊断、病理类型的推断中具有重要意义,而联合检测更有助于肺癌的诊断.%Objective To evaluate the clinical value of combined determination of tumor markers including serum squamous cell carcinoma antigen(SCCA) ,cytokeratin 19 fragment(CYFRA21-l) , carcino-embryonic antigen(CKA) in diagnosis of lung cancer. Methods 63 cases including 44 cases of lung cancer and 19 benign lesion were collected from inpatients registered in our hospital from January to April in 2008. The lung cancer were pathologically diagnosed including 5 cases of small cell lung cancer, 17 cases of squamous cell carcinoma ,15 cases of adenocarcinoma,and 7 undecided type lung cancer. All cases were bronchoalveolar lavaged and bronchoalveolar lavage fluid were collected to prepare for detect SCCA, CYFRA21-1, CEA. Results The levels of the 3 tumor markers measured in serum were higher in lung cancer group than those in benign lung disease group(P<0. 01). The tumor markers increased to different degrees among the patients in various pathological classifications. The levels of SCCA ,CYFRA21-1 measured in serum were higher in squamous cell carcinoma group, and the levels of CEA were higher in adenocarcinoma group(P< 0. 01). Conclusion The

  13. Virosomes for antigen and DNA delivery

    NARCIS (Netherlands)

    Daemen, T; de Mare, A; Bungener, L; de Jonge, J; Huckriede, A; Wilschut, J

    2005-01-01

    Specific targeting and delivery as well as the display of antigens on the surface of professional antigen-presenting cells (APCs) are key issues in the design and development of new-generation vaccines aimed at the induction of both humoral and cell-mediated immunity. Prophylactic vaccination agains

  14. Protein antigen delivery by gene gun-mediated epidermal antigen incorporation (EAI).

    Science.gov (United States)

    Scheiblhofer, Sandra; Ritter, Uwe; Thalhamer, Josef; Weiss, Richard

    2013-01-01

    The gene gun technology can not only be employed for efficient transfer of gene vaccines into upper layers of the skin, but also for application of protein antigens. As a tissue rich in professional antigen presenting cells, the skin represents an attractive target for immunizations. In this chapter we present a method for delivery of the model antigen ovalbumin into the skin of mice termed epidermal antigen incorporation and describe in detail how antigen-specific proliferation in draining lymph nodes can be followed by flow cytometry.

  15. Efeito da cobertura viva com leguminosas herbáceas perenes na agregação de um argissolo

    Directory of Open Access Journals (Sweden)

    A. Perin

    2002-09-01

    Full Text Available O presente trabalho foi desenvolvido na área do Campo Experimental da Embrapa Agrobiologia, Seropédica (RJ, com o objetivo de avaliar a morfologia e a distribuição de raízes de algumas leguminosas herbáceas perenes; os efeitos da cobertura viva no teor de carbono orgânico, e a agregação de um Argissolo Vermelho-Amarelo distrófico, medida pela estabilidade dos agregados em água. O delineamento experimental adotado foi o de blocos casualizados, com três repetições. Os tratamentos consistiram de três diferentes espécies de leguminosas herbáceas perenes e um tratamento-controle sem cobertura viva (capinado. As leguminosas utilizadas foram amendoim forrageiro (Arachis pintoi, cudzu tropical(Pueraria phaseoloides e siratro (Macroptilium Atropurpureum. Para a estabilidade de agregados, as profundidades de amostragem foram 0-5 e 5-10 cm, enquanto, para a morfologia e distribuição radicular, as avaliações consistiram das profundidades 0-5, 5-10, 10-20 e 20-40 cm. As coberturas com as leguminosas amendoim forrageiro e cudzu tropical propiciaram os maiores valores percentuais na classe de agregados > 2,00 mm, em média 38 % superiores aos obtidos na área capinada. Os valores do diâmetro médio ponderado (DMP dos agregados no solo com cobertura de leguminosas foram superiores aos da área capinada para ambas as camadas, o que demonstra o efeito favorável das coberturas vivas na estabilização dos agregados do solo. A cobertura viva de amendoim forrageiro proporcionou incremento no teor de carbono orgânico no solo. Quanto aos atributos morfológicos das raízes, verificou-se que o amendoim forrageiro apresentou raio radicular intermediário entre as demais espécies e área e massa radicular maiores, o que auxiliou na interpretação do efeito positivo da cobertura viva com essa espécie na agregação do solo.

  16. Cerium oxide-deposited mesoporous silica nanoparticles for the determination of carcinoembryonic antigen in serum using inductively coupled plasma-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Choi, H.W. [Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701 (Korea, Republic of); Lee, K.H.; Hur, N.H. [Department of Chemistry, Sogang University, Shinsu-dong, Mapo-gu, Seoul (Korea, Republic of); Lim, H.B., E-mail: plasma@dankook.ac.kr [Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701 (Korea, Republic of)

    2014-10-17

    Highlights: • Sandwich-type immunoassay using ICP-MS and nanoparticles to determine biomarkers. • CeO{sub 2}-deposited mesoporous silica nanoparticles were synthesized as a probe. • Ratiometric measurement significantly improved the calibration linearity. • Excellent detection limit was achieved by signal amplification. - Abstract: CeO{sub 2}-deposited mesoporous silica nanoparticles were synthesized as a probe to determine carcinoembryonic antigen (CEA) in serum by inductively coupled plasma-mass spectrometry (ICP-MS). The prepared mesoporous nanoparticles were modified and tagged to the target for sandwich-type immunoassay. Fe{sub 3}O{sub 4} magnetic nanoparticles (MNPs) were also synthesized and immobilized with antibody to extract the target biomarker. The calibration curve of the synthesized CeO{sub 2}-deposited silica nanoparticles, which was plotted by the signal ratio of {sup 140}Ce/{sup 57}Fe measured by ICP-MS vs. the concentration of CEA, showed excellent linearity and sensitivity owing to the signal amplification and low spectral interference. Under optimal conditions, the sandwich-type analytical method was applied to determine CEA in serum spiked in the range of 0.001–5 ng mL{sup −1} and showed a limit of detection of 0.36 ng mL{sup −1}. Since the deposited CeO{sub 2} in the mesoporous silica layer can be substituted by other metal compounds, various kinds of metal-deposited nanoparticles can be prepared as probe materials for multiplex detection in bioanalysis.

  17. Expression and clinical significance of CEA-mRNA, CK20-mRNA in peripheral blood of esophageal cancer patients%食管癌患者外周血CEA-mRNA、CK20-mRNA的表达及意义

    Institute of Scientific and Technical Information of China (English)

    金文波; 周乃康; 郑梦利; 祁彦君; 马连君

    2009-01-01

    目的 以CEA-mRNA、CK20-mRNA为靶基因,应用逆转录聚合酶链反应(RT-PCR)的方法,检测食管癌患者肿瘤组织以及食管癌患者外周血微转移,探寻靶基因作为食管癌微转移检测的分子标志物的可行性.方法 应用RT-PCR技术检测53例食管癌组织中CEA-mRNA、CK20-mRNA的表达;检测53例食管癌患者外周血中靶基因的表达,并以10例良性食管疾病患者和20名健康人外周血作为对照.结果 53例食管癌患者病理组织中CEA-mRNA、CK20-mRNA的表达率为96.23%(51/53)、100%(53/53),外周血中CEA-mRNA、CK20-mRNA表达阳性率为52.83%(28/53)、49.06%(26/53);对照组中,10例食管良性病变患者和20名健康人外周血中均有1例CEA-mRNA表达、无CK20-mRNA表达.结论 CEA-mRNA、CK20-mRNA是检测食管癌组织及食管癌外周血微转移良好的分子标志物,两者表达与食管癌TNM分期关系密切.%Objective To establish reverse transcriptase-polymerase chain reaction(RT-PCR) with primers specific for CEA gene and CK20 gene to detect circulating tumor cells in peripheral blood of esophageal cancer pa-tients,and try to find the relationship between the mRNA expression and micrometastasis. Methods The expressions of CEA,CK20 were analyzed by RT-PCR in 53 cases of esophageal tumor tissue and in peripheral blood,compared with 10 patients with benign esophageal disease and 20 healthy volunteers. Results The expressions of CEA-mRNA, CK20-mRNA were 96.23% (51/53), 100% ( 53/53 ) in 53 esophageal tumor tissue and were 52.83% (28/53), 49.06% (26/53) in peripheral blood of 53 esophageal cancer patients. In control group,there was only one expression of CEA-mRNA in peripheral blood of 10 patients with benign esophageal disease,as well as in 20 healthy volunteers. There was no expression of CK20-mRNA in peripheral blood of 10 patients with benign esophageal disease and 20 healthy volunteers. Conclusion CEA-mRNA, CK20-mRNA might be specific and sensitive markers to detect

  18. Further characterization of filarial antigens by SDS polyacrylamide gel electrophoresis

    OpenAIRE

    Dissanayake, S.; Galahitiyawa, S. C.; Ismail, M. M.

    1983-01-01

    SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis of an antigen isolated from sera of Wuchereria bancrofti-infected patients and Setaria digitata antigen SD2-4 is reported. Both antigens showed carbohydrate (glycoprotein) staining. The W. bancrofti antigen had an apparent relative molecular mass of 35 000 while the S. digitata antigen SD2-4 migrated at the marker dye position on SDS-polyacrylamide gel electrophoresis. SDS treatment of these antigens did not abolish the precipita...

  19. Review of nuclear data improvement needs for nuclear radiation measurement techniques used at the CEA experimental reactor facilities

    Directory of Open Access Journals (Sweden)

    Destouches Christophe

    2016-01-01

    Full Text Available The constant improvement of the neutron and gamma calculation codes used in experimental nuclear reactors goes hand in hand with that of the associated nuclear data libraries. The validation of these calculation schemes always requires the confrontation with integral experiments performed in experimental reactors to be completed. Nuclear data of interest, straight as cross sections, or elaborated ones such as reactivity, are always derived from a reaction rate measurement which is the only measurable parameter in a nuclear sensor. So, in order to derive physical parameters from the electric signal of the sensor, one needs specific nuclear data libraries. This paper presents successively the main features of the measurement techniques used in the CEA experimental reactor facilities for the on-line and offline neutron/gamma flux characterizations: reactor dosimetry, neutron flux measurements with miniature fission chambers and Self Power Neutron Detector (SPND and gamma flux measurements with chamber ionization and TLD. For each technique, the nuclear data necessary for their interpretation will be presented, the main identified needs for improvement identified and an analysis of their impact on the quality of the measurement. Finally, a synthesis of the study will be done.

  20. EURATOM-CEA association contributions to the 26. EPS conference on controlled fusion and plasma physics, Maastricht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-10-15

    This report references the EURATOM-CEA association contributions presented at the 26. EPS conference on controlled fusion and plasma physics, in Maastricht (Netherlands) the 14-18 June 1999. Two invited papers and 24 contributed papers are proposed. They deal with: tokamak devices; particle recirculation in ergodic divertor; current profile control and MHD stability in Tore Supra discharges; edge-plasma control by the ergodic divertor; electron heat transport in stochastic magnetic layer; bolometry and radiated power; particle collection by ergodic divertor; study and simulation of pa impurities; line shape modelling for plasma edge conditions; dynamical study of the radial structure of the fluctuations measured by reciprocating Langmuir probe in Tore Supra; up-down asymmetry of density fluctuations; Halo currents in a circular tokamak; real time measurement of the position, density, profile and current profile at Tore Supra; poloidal rotation measurement by reflectometry; interpretation of q-profile dependence of the LH power deposition profile during LHCD experiments; ICFR plasma production and optimization; improved core electron confinement; measurement of hard X-ray emission profile; modelling of shear effects on thermal and particles transport; ion turbulence; current drive generation based on autoresonance and intermittent trapping mechanisms. (A.L.B.)

  1. Caracterização físico-química de algumas tuberosas amiláceas

    Directory of Open Access Journals (Sweden)

    Leonel Magali

    2002-01-01

    Full Text Available As principais fontes de amido comercial no mundo são o milho, a batata e a mandioca. Entretanto, nos últimos anos vem crescendo o interesse em amidos naturais que possam ser utilizados pela indústria alimentícia. Assim, este trabalho teve por objetivo caracterizar, quanto à composição físico-química, açafrão, ahipa, araruta, batata-doce, biri, inhame e mandioquinha-salsa, tuberosas amiláceas potencialmente passíveis de introdução como matérias-primas de interesse comercial. Amostras das tuberosas foram analisadas quanto ao teor de umidade, cinzas, proteína, matéria graxa, açúcares redutores e totais, fibras e amido. Os resultados obtidos mostraram que das tuberosas analisadas as que apresentaram maior teor de amido foram a araruta (Maranta arundinacea, o inhame (Dioscorea sp e o biri (Canna edulis, sendo que o inhame e o biri apresentaram também o maior rendimento potencial em toneladas de amido/hectare.

  2. Review of nuclear data improvement needs for nuclear radiation measurement techniques used at the CEA experimental reactor facilities

    Science.gov (United States)

    Destouches, Christophe

    2016-03-01

    The constant improvement of the neutron and gamma calculation codes used in experimental nuclear reactors goes hand in hand with that of the associated nuclear data libraries. The validation of these calculation schemes always requires the confrontation with integral experiments performed in experimental reactors to be completed. Nuclear data of interest, straight as cross sections, or elaborated ones such as reactivity, are always derived from a reaction rate measurement which is the only measurable parameter in a nuclear sensor. So, in order to derive physical parameters from the electric signal of the sensor, one needs specific nuclear data libraries. This paper presents successively the main features of the measurement techniques used in the CEA experimental reactor facilities for the on-line and offline neutron/gamma flux characterizations: reactor dosimetry, neutron flux measurements with miniature fission chambers and Self Power Neutron Detector (SPND) and gamma flux measurements with chamber ionization and TLD. For each technique, the nuclear data necessary for their interpretation will be presented, the main identified needs for improvement identified and an analysis of their impact on the quality of the measurement. Finally, a synthesis of the study will be done.

  3. Development and manufacturing of a Nb$_{3}$Sn quadrupole magnet Model at CEA/Saclay for TESLA Interaction Region

    CERN Document Server

    Durante, Maria; Fratini, M; Leboeuf, D; Segreti, M; Védrine, Pierre; 10.1109/TASC.2004.829129

    2004-01-01

    One possible application of Nb/sub 3/Sn, whose superconducting properties far exceed those of NbTi, is the fabrication of short and powerful quadrupole magnets for the interaction regions of large particle accelerators. In some projects, as in the future linear collider TESLA, the quadrupole magnets are inside the detector solenoid and must operate in its background field. This situation gives singular Lorentz force distribution in the ends of the magnet. To learn about Nb/sub 3/Sn technology, evaluate fabrication techniques and test the interaction with a solenoidal field, DAPNIA /SACM at CEA/Saclay has started the manufacturing of a 1-m-long, 56- mm-single-aperture quadrupole magnet model. The model relies on the same coil geometry as the LHC arc quadrupole magnets, but has no iron yoke. It will produce a nominal field gradient of 211 T/m at 11,870 A. The coils are wound from Rutherford-type cables insulated with glass fiber tape, before being heat-treated and vacuum-impregnated with epoxy resin. Laminated,...

  4. Kinetics and immunocytochemical dyeing of monoclonal 99mTc-labelled anti-CEA-antibodies against granulocytes after intravenous administration

    International Nuclear Information System (INIS)

    Until now the clinical identification of the affinity of monoclonal 99mTc-anti-CEA antibodies (MAK BW 250/183) on granulocytes was made with tumor cells carrying the same epitope on NCA-95 and human granulocytes in vitro. As this antibody only binds human granulocytes in vitro. As this antibody only binds human granulocytes, animal experiments are impossible. 3 patients had their blood withdrawn within 6 h after injection, another patient had his left hip-joint biopsied after 24 h, the samples undergoing subsequent immunocytochemical dyeing. Dyeing of granulocytes all over the smears was evident whereas lymphocytes, monocytes and erythrocytes did not show any reaction. After 6 h there seemed to be a large difference between a relatively high quantity of 86% unlabelled 99mTc-MAb and 75% of immunocytochemically stainable granulocytes in the blood through an excess of binding epitopes. Six h after injection 27% of the activity were, on average, detectable in whole blood. At this time the activity in blood was reduced to an extent that scintigraphic imaging was feasible. (orig.)

  5. Principais doenças de Anonáceas no Brasil: descrição e controle

    Directory of Open Access Journals (Sweden)

    Nilton Tadeu Vilela Junqueira

    2014-01-01

    Full Text Available Várias doenças podem afetar folhas, caules, flores e frutos de gravioleira, pinheira e atemoia em diferentes estádios de seus desenvolvimentos. Geralmente, as doenças mais importantes são causadas por fungos durante o florescimento e a frutificação. Também podem ocorrer murchas ou podridões de raízes, causadas por nematoides e patógenos do solo. A antracnose (Colletotrichum gloeosporioides, murcha ou podridões de raízes (Rhizoctonia solani, Cylindrocladium clavatum, Phytophthora sp., Pythium sp., Phytophthora nicotianae var. parasitica, cancros (Albonectria rigidiuscula e podridão de frutos (Botryodiplodia theobromae, sin. Lasiodiplodia theobromae são as mais importantes. Por outro lado, a podridão-parda-do-fruto (Rhizopus stolonifer provoca perdas expressivas na produção de graviola. A seguir, são descritas as principais doenças que afetam estas espécies de anonáceas, seus agentes causais e as medidas de controle.

  6. Comparing Prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins Glypican-3, Alpha feto protein and Carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients

    International Nuclear Information System (INIS)

    Background: Hepatocellular carcinoma (HCC) is usually asymptomatic in the early stage and does not show elevated alpha-feto protein (AFP). AFP shows 60-80% sensitivity in diagnosing HCC. Glypican3 (GPC-3) is an oncofetal protein that is only detected in HCC cells but not in benign liver tissues, while Carcinoembryonic antigen (CEA) is expressed in various neoplasms including HCC. Although, it is not specific for HCC. Prothrombin induced by vitamin K absence-II (PIVKA-II) is an abnormal prothrombin protein that is increased in the serum of HCC patients. It has higher sensitivity and specificity compared to AFP. The aim of this study is to compare the clinical utility of PIVKA-II with GPC-3, AFP and CEA in diagnosing HCC. Prothrombin induced by vitamin K absence-II Patients and methods: This study included 40 patients with HCC, 10 patients with cirrhosis as a benign control group, and 10 apparently healthy volunteers as normal controls. Serum samples were subjected to routine laboratory investigations, measurement of CEA, AFP using MEIA technique (Axsym), glypican3, and PIVKA-II using ELISA technique in the sera of all patients and controls. Results: All markers showed the highest results in the HCC group. Higher concentrations of PIVKA- II were detected in patients with splenomegaly, and in tumors with size (>3 cm). Combination of Glypican-3 and PIVKA-II showed the highest sensitivity, while GPC-3 alone and combination of GPC-3 and AFP showed the highest specificity to differentiate HCC from liver cirrhosis and normal controls. GPC-3, PIVKAII, and combination of both showed the highest sensitivity, while GPC-3 alone showed the highest specificity to differentiate HCC from liver cirrhosis. Conclusion: Glypican-3 is the only oncofetal antigen that showed comparable high diagnostic accuracy as PIVKA-II in diagnosing HCC among Egyptian patients.

  7. Presentation of the CEA's crisis national organization: coordination centre in case of crisis, crisis technical teams, intervention means, and so on

    International Nuclear Information System (INIS)

    After having briefly recalled the existence of a legal framework for crisis management organisation, this report briefly describes how the CEA plans the crisis management. This management is based on the definition of critical scenarios, on the building up of a crisis management team, and on the elaboration of crisis management operational documents. It evokes the alert organisation and the triggering of crisis management. Then, it describes the CEA's national crisis organisation with its main crisis management structures, the role and the operation of the Crisis Coordination Centre (CCC, the decision body), the role and operation of the Central Crisis Technical Teams (ETC-C, Equipes Techniques de Crise Centrales), the role of field interveners (various rescue, protection, health care and technical teams) and of other additional intervention actors. It evokes the objectives of the various exercises which are organised every year at the internal, national or international level

  8. The CEA Cadarache site. Additional safety assessment with respect to the accident which occurred in the Fukushima-Daiichi nuclear power station

    International Nuclear Information System (INIS)

    After a presentation of some characteristics of the CEA Cadarache site (internal and external industrial environment, crisis management organization at the CEA level and at the local level), this document reports the identification of structures and equipment concerned by crisis management (site support functions, critical structures and equipment concerned by additional safety assessments). Then, it addresses the different risks: earthquake (sizing of critical structures and equipment, margin assessment), flooding (possible origins, alarm measures), other extreme natural events (extreme meteorological conditions, extreme earthquake with induced flooding, forest fire), and loss of electric supplies and of cooling systems. The last parts address the organization of accident management in situation typically related to additional safety management), and subcontracting conditions and practices

  9. Significance of CEA, CA15-3 and biochemical markers of bone turnover in the diagnosis of bone metastasis from breast cancer

    International Nuclear Information System (INIS)

    Objective: To evaluate the significance of tumor markers CEA and CA15-3, and biochemical markers of bone turnover (total procollagen type Ⅰ amino-terminal propeptide (TP Ⅰ NP), β-isomerized carboxyterminal propeptide (β-CTx), ALP and PTH) in the diagnosis of bone metastasis from breast cancer. Methods: A total of 78 patients (all females) with mean age (56.72 ± 10.76) years, who were diagnosed with breast cancer, were included in this study. The patients were divided into two groups based on radionuclide bone imaging: with bone metastasis (n=32) and without bone metastasis (n=46). The serum concentrations of CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH, ALP were measured. Gleason scores were evaluated. The diagnostic value was evaluated by ROC curve.The two groups were compared using two-sample t test. The correlations between bone metastasis and tumor markers, bone metastasis and biochemical markers of bone turnover were analyzed with Pearson correlation and logistic analysis. Results: The serum levels of CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH and ALP were significantly higher in the group with bone metastasis than those in the group without bone metastasis (t: 4.16-7.56, all P<0.05). For the diagnosis of bone metastasis from breast cancer, the AUC of CEA, CA15-3, TP Ⅰ NP, [β-CTx, PTH and ALP was 0.815, 0.887, 0.869, 0.852, 0.844, 0.731, respectively. Using the cut-off values of 4.18 μg/L for CEA, 0.04 U/L for CA15-3, 49.70 μg/L for TP Ⅰ NP, 0.47 pg/L for β-CTx,54.90 ng/L for PTH and 49.90 U/L for ALP, the sensitivities were 56.3% (18/32), 75.0% (24/32), 78.1% (25/32), 81.3% (26/32), 78.1% (25/32), 68.8% (22/32) and the specificities were 80.4% (37/46), 84.8% (39/46), 76.1% (35/46), 78.3% (36/46), 69.6% (32/46), 58.7% (27/46), respectively. CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH, ALP and Gleason score were positively correlated with the presence of bone metastasis (r: 0.267-0.636, all P<0.05). CEA, CA15-3, TP Ⅰ NP, β-CTx, PTH and Gleason score were independent

  10. Meningococcal vaccine antigen diversity in global databases.

    Science.gov (United States)

    Brehony, Carina; Hill, Dorothea M; Lucidarme, Jay; Borrow, Ray; Maiden, Martin C

    2015-01-01

    The lack of an anti-capsular vaccine against serogroup B meningococcal disease has necessitated the exploration of alternative vaccine candidates, mostly proteins exhibiting varying degrees of antigenic variation. Analysis of variants of antigen-encoding genes is facilitated by publicly accessible online sequence repositories, such as the Neisseria PubMLST database and the associated Meningitis Research Foundation Meningococcus Genome Library (MRF-MGL). We investigated six proposed meningococcal vaccine formulations by deducing the prevalence of their components in the isolates represented in these repositories. Despite high diversity, a limited number of antigenic variants of each of the vaccine antigens were prevalent, with strong associations of particular variant combinations with given serogroups and genotypes. In the MRF-MGL and globally, the highest levels of identical sequences were observed with multicomponent/multivariant vaccines. Our analyses further demonstrated that certain combinations of antigen variants were prevalent over periods of decades in widely differing locations, indicating that vaccine formulations containing a judicious choice of antigen variants have potential for long-term protection across geographic regions. The data further indicated that formulations with multiple variants would be especially relevant at times of low disease incidence, as relative diversity was higher. Continued surveillance is required to monitor the changing prevalence of these vaccine antigens. PMID:26676305

  11. A network signal amplification strategy of ultrasensitive photoelectrochemical immunosensing carcinoembryonic antigen based on CdSe/melamine network as label.

    Science.gov (United States)

    Li, Jiaojiao; Zhang, Yong; Kuang, Xuan; Wang, Zhiling; Wei, Qin

    2016-11-15

    Taking advantage of CdSe/melamine network as label and Au-TiO2 as substrate, this work developed a novel kind of signal amplification strategy for fabricating photoelectrochemical (PEC) immunoassay. The melamine, a star-shaped triamino molecule, was firstly used for readily capturing CdSe QDs and forming a CdSe/melamine network, which was formed through strong interactions between the carboxyl groups of TGA-stabilized CdSe QDs and the three amino groups of each melamine molecule. In this strategy, the primary antibody (Ab1) was immobilized onto Au-TiO2 substrate, which made the photoelectric conversion efficiency increase significantly. After the formed Ab2-CdSe/melamine network labels were captured onto the electrode surface via the specific antibody-antigen interaction, the photoelectric activity could be further enhanced via the interaction between the Au-TiO2 substrate and CdSe/melamine network. Due to this amplification of PEC signals and the special structure of the label, the fabricated PEC immunosensor was applied for sensitive and specific detection of cancer biomarker carcinoembryonic antigen (CEA), and displayed a wide linear range (0.005-1000ngmL(-1)) and low detection limit (5pgmL(-1)). In addition, the immunosensor was performed with good stability and reproducibility, and the results to analyze human serum samples were satisfactory. PMID:27281106

  12. Disolución y génesis del karst en rocas carbonáticas y rocas silíceas : un estudio comparado.

    OpenAIRE

    GALAN, Carlos

    1991-01-01

    Se describen los mecanismos físicos y químicos que   intervienen en la formación del karst en rocas silíceas y   carbonáticas. Los modelados resultantes son comparados. Se   analizan los factores hidrogeológicos, se cuantifica el trabajo   de excavación y se discute la edad de las formas.

  13. Effect of radiotherapy on serum SCC, CEA, CRFRA21-1, TAG72, CA199 and lymphocyte subsets in patients with esophageal squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    Sha Sha; Bo Yu; Zhong-Qin Shu; Xiao-Wei Gu; Wei-Dong Mao; Lin-Yun Xia; Jian-Jun Qin

    2016-01-01

    Objective:To study the effect of radiotherapy on serum SCC, CEA, CRFRA21-1, TAG72, CA199 and lymphocyte subsets in patients with esophageal squamous cell carcinoma. Methods: A total of 60 patients with esophageal squamous cell carcinoma in our hospital from January 2013 to January 2016 were selected as experiment group and 40 healthy subjects were selected as control group. Patients in experiment group were treated with 6MV X-ray radiation therapy. Serum SCC, CEA, CRFRA21-1, TAG72, CA199 and the cell percentage of peripheral blood CD4+, CD8+ were compared in control group and the experimental group before and after 1 month radiotherapy.Results:Before treatment, the levels of serum SCC, CEA and CRFRA21-1 in the experimental group were significantly higher than those in the control group (P0.05). Before treatment, the cell percentage of peripheral blood CD4+, CD8+ and the ratio of CD4+/CD8+ in experimental group was significantly lower than that of the control group, the percentage of peripheral blood CD8+ in the experimental group was significantly higher than that in the control group (P0.05), and in the experimental group, the proportion of CD4+ cells and the tatio of CD4+/CD8+in peripheral blood was significantly lower than that of the control group, the proportion of CD8+ was significantly higher than that of the control group (P<0.05).Conclusions: Radiotherapy can significantly reduce the serum SCC, CEA, CRFRA21-1, TAG72 and CA199 levels of the patients with esophageal squamous cell carcinoma, but have less influence on the T lymphocyte subsets.

  14. Radioimmunoimaging of non-small cell lung cancer with [sup 111]In- and [sup 99m]Tc-labeled monoclonal anti-CEA antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Kairemo, K.J.A. (Dept. of Clinical Chemistry, Helsinki Univ. Central Hospital (Finland)); Aronen, H.J. (Dept. of Radiology, Helsinki Univ. Central Hospital (Finland)); Liewendahl, K. (Dept. of Clinical Chemistry, Helsinki Univ. Central Hospital (Finland)); Paavonen, T. (Dept. of Pathology, Helsinki Univ. Central Hospital (Finland)); Heikkonen, J.J. (Dept. of Radiotherapy and Oncology, Helsinki Univ. Central Hospital (Finland)); Virkkunen, P. (Dept. of Radiotherapy and Oncology, Helsinki Univ. Central Hospital (Finland)); Maeki-Hokkonen, H. (Dept. of Radiotherapy and Oncology, Helsinki Univ. Central Hospital (Finland)); Karonen, S.L. (Dept. of Clinical Chemistry, Helsinki Univ. Central Hospital (Finland)); Brownell, A.L. (Dept. of Clinical Chemistry, Helsinki Univ. Central Hospital (Finland)); Maentylae, M.J. (Dept. of Radiotherapy and Oncology, Helsinki Univ. Central Hospital (Finland))

    1993-01-01

    Radiolabeled monoclonal anti-CEA antibodies were used for radioimmunolocalization (RIL) of non-small cell lung cancer; in 30 patients with [sup 111]In labeled anti CEA F(ab')[sub 2] fragment (BW 431/31) and in 16 with [sup 99m]Tc-labeled intact MoAb (BW 431/26). RIL results were compared with those of other imaging modalities. Paraffin sections from some patients were also studied immunohistochemically using anti-CEA antibody. Patients with [sup 111]In labeled MoAB were imaged twice 1-4 days after injection and for image enhancement pulmonary and liver/spleen subtraction were performed. Twenty-seven of 28 primary tumors were positive and metastases were detected in all patients. The total number of lesions was 78 of which 61 (78%) could be detected by RIL. For verification CT was applied to the study of 46 lesions detected by RIL. We found 6 unknown lesions subsequently verified histologically. Using subtraction techniques we detected 9 lesions in 4 patients, later verified as plumonary metastases, not detected in unprocessed images. Pleural, mediastinal and pericardial lesions were also better delineated in subtracted images than in unprocessed images. Imaging of non-small cell lung cancer with [sup 99m]Tc-labeled MoAB was performed twice 4-24 h after injection. RIL results were compared with other imaging methods; CT US, conventional radiography, and immunohistochemistry. Twelve out of 16 patients with suspected or known lung cancer had positive immunoscintigrams; 19 of 25 lesions could be detected by RIL. There were 5 false positive and 2 true negative findings. Immunoperoxidase (IP) stainings of paraffin sections of the tumours from 7 patients were performed using two different anti-CEA antibodies; BW 431/26 and ZCEA[sub 1]. None of the seven tumors examined by immunohistochemistry were negative when stained by BW 431/26, which was the antibody used for immunoscintigraphy. (orig.).

  15. Tumor localization of radiolabeled antibodies against carcinoembryonic antigen in patients with carcinoma

    International Nuclear Information System (INIS)

    Purified, [131I]-labeled goat antibodies against carcinoembryonic antigen, which have been shown to localize in human carcinoma in nude mice, were injected into 27 patients with carcinoma. Patients were scanned with a scintillation camera at various intervals. In 11 patients, radioactivity was detectable in the tumor 48 h after injection. Computerized subtraction of blood-pool radioactivity provided clearer pictures in positive cases, but in 16 patients the scans remained doubtful or negative. To study the specificity of [131]-antibody localization, we gave some patients simultaneous injections of [125]-labeled normal IgG. Both isotopes were measured by means of scintillation counting in tumors and normal tissues recovered after surgery. The results demonstrated that only the anti-CEA antibodies localized in tumors. However, the total antibody-derived radioactivity in the tumor was only about 0.001 of the injected dose. We conclude that, despite the present demonstration of specificity, this method of tumor detection is not yet clinically useful

  16. Antigen incorporation on Cryptosporidium parvum oocyst walls

    Directory of Open Access Journals (Sweden)

    Entrala Emilio

    2001-01-01

    Full Text Available Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (Merifluor®, Meridian Diagnostic Inc. could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism.

  17. From moon stones to simulation - More than 40 years of experiments on the 7 MV tandem accelerator at Cea/Dam Ile de France; Des pierres de lune a la simulation - Plus de 40 ans d'experiences aupres de l'accelerateur Van de Graaff tandem 7 MV CEA/DAM Ile de France

    Energy Technology Data Exchange (ETDEWEB)

    Bauge, E.; Bersillon, O.; Couillaud, Ch.; Daugas, J.M.; Flament, J.L.; Frehaut, J.; Haouat, G.; Joly, S.; Laborie, J.M.; Ledoux, X.; Marmouget, J.G.; Patin, Y.; Poncy, J.L.; Sigaud, J.; Varignon, C

    2009-07-01

    Following the decommissioning of the 7 MV tandem accelerator of CEA/DAM by the end of 2007, the present report relates the history of the accelerator from its beginning in the sixties and then the nuclear physics studies on fission, (x,xn) reactions, elastic and inelastic scattering, etc and other studies in microelectronics, biology, etc) performed with that installation. (authors)

  18. Prostate-specific antigen (PSA) blood test

    Science.gov (United States)

    Prostate-specific antigen; Prostate cancer screening test; PSA ... PSA testing is an important tool for detecting prostate cancer, but it is not foolproof. Other conditions can cause a rise in PSA, including: A larger prostate ...

  19. Mapping Epitopes on a Protein Antigen by the Proteolysis of Antigen-Antibody Complexes

    Science.gov (United States)

    Jemmerson, Ronald; Paterson, Yvonne

    1986-05-01

    A monoclonal antibody bound to a protein antigen decreases the rate of proteolytic cleavage of the antigen, having the greatest effect on those regions involved in antibody contact. Thus, an epitope can be identified by the ability of the antibody to protect one region of the antigen more than others from proteolysis. By means of this approach, two distinct epitopes, both conformationally well-ordered, were characterized on horse cytochrome c.

  20. Neuropeptide Y (NPY) in the central nucleus of the amygdala (CeA) does not affect ethanol-reinforced responding in binge-drinking, nondependent rats.

    Science.gov (United States)

    Henderson, Angela N; Czachowski, Cristine L

    2012-03-01

    The central nucleus of the amygdala (CeA) has been implicated as having a significant role in mediating alcohol-drinking behavior. Neuropeptide Y (NPY) has been investigated as a potential pharmacotherapeutic due to its ability to attenuate ethanol intake, particularly when administered into the CeA. Previous research suggests, though the evidence is somewhat conflicting, that the efficacy of NPY is contingent upon genetic background and/or prior history of ethanol dependence in rats. However, studies looking at the effects of NPY in nonselected animals lacking a history of ethanol dependence have two factors that could impact the interpretation of the results: ethanol history/selection AND relatively low baseline ethanol intakes as compared to ethanol-dependent and/or genetically selected controls. The purpose of the present study was to generate higher baseline ethanol intakes upon which to examine the effects of NPY on ethanol and sucrose drinking in nonselected rats using a binge drinking model. Long Evans rats were trained to complete a single response requirement resulting in access to either 2% sucrose (Sucrose Group) or 2% sucrose/10% ethanol (Ethanol Group) for a 20-min drinking session. On treatment days, rats were bilaterally microinjected into the CeA with aCSF or one of three doses of NPY (0.25μg, 0.50μg, or 1.00μg/.5μL). Subjects in the Ethanol Group were consuming an average of 1.2g/kg of ethanol (yielding BELs of ~90mg%) during the 20min access period following aCSF treatments. The results revealed that NPY had no effect on either sucrose or ethanol consumption or on appetitive responding (latency to respond). Overall, the findings indicate that even a history of binge-like ethanol consumption is not sufficient to recruit CeA NPY activity, and are consistent with previous studies showing that the role of NPY in regulating ethanol reinforcement in the CeA may be contingent upon a prior history of ethanol dependence.

  1. Tales of Antigen Evasion from CAR Therapy.

    Science.gov (United States)

    Sadelain, Michel

    2016-06-01

    Both T cells bearing chimeric antigen receptors and tumor-specific antibodies can successfully target some malignancies, but antigen escape can lead to relapse. Two articles in this issue of Cancer Immunology Research explore what effective countermeasures may prevent it. Cancer Immunol Res; 4(6); 473-473. ©2016 AACRSee articles by Zah et al., p. 498, and Rufener et al., p. 509. PMID:27252092

  2. Characterization of an antigenically distinct porcine rotavirus.

    OpenAIRE

    Bridger, J C; Clarke, I. N.; McCrae, M A

    1982-01-01

    A porcine virus with rotavirus morphology, which was antigenically unrelated to previously described rotaviruses, is described. Particles with an outer capsid layer measured 75 nm and those lacking the outer layer were 63 nm in diameter. Particles which resembled cores were also identified. The virus was shown to be antigenically distinct from other rotaviruses as judged by immunofluorescence and immune electron microscopy, and it failed to protect piglets from challenge with porcine rotaviru...

  3. Rooting of herbaceous minicuttings of passion fruit/ Enraizamento de miniestacas herbáceas de maracujazeiro amarelo

    Directory of Open Access Journals (Sweden)

    Rudival Faquim

    2007-08-01

    Full Text Available The objective of this work was to evaluate the effect of the basal cut, leaf presence and the treament with a root suspension in the rooting of herbaceous minicuttings of yellow passion fruit. The experiment was realized in a greenhouse using minicuttings with 8 cm of lenght and two nodes, collected from yellow passion fruit seedlings. The longitudinal basal cut in the minicuttings was done until the basal node. The leaves were removed or a half of a single leaf was maintained in the apical node. The minicuttings were treated with a root suspension in water in three concentrations (0, 100 e 200 g L-1. After 58 days, the rooting percentage, the number of roots and the root dry matter produced per minicutting were determined. The entirely randomized experimental design was used in a factorial esqueme (2 x 2 x 3 with three replications. The propagation of the yellow passion fruit by herbaceous minicuttings was viable and the presence of the half of a single leaf in the apical node give a higher production of number and dry matter of roots. The basal cut was only a little favourable to the production of roots and the treatment with a root suspension did not present a benefit to the plant propagation.Este trabalho objetivou avaliar a influência do corte basal, da presença da folha e do tratamento com suspensão de raízes no enraizamento de miniestacas herbáceas de maracujazeiro amarelo. O experimento foi conduzido em casa de vegetação com miniestacas de 8 cm de comprimento e dois nós, obtidas de mudas de maracujazeiro. O corte longitudinal na base das estacas foi feito até o nó basal. Foram retiradas as folhas ou mantida a metade da folha do nó apical. As miniestacas foram tratadas com suspensão de raízes mais água nas concentrações de 0, 100 e 200 g L-1. Após 58 dias, determinou-se a porcentagem de estacas enraizadas, o número de raízes emitidas e a massa seca de raízes por estaca. O delineamento experimental utilizado foi o

  4. Construction of predictive models for radionuclide sorption applied to radioactive waste deep storage: an overview of the CEA research program

    International Nuclear Information System (INIS)

    Full text of publication follows: Deep geological storage is studied in France as one of the three potential options for managing long lived nuclear waste in the framework of the 1991 Law. One of the key topics of research deals with the behaviour of radionuclides (RN) in the geological environment, focusing in particular on the retention at the solid/water interfaces (in the engineered barriers or within the host rock), the diffusion process within the rock as well as the coupling between chemistry and transport processes. The final aim is to develop validated and reliable long-term predictive migration models. These researches are mainly coping with Callovo-Oxfordian argilites and near field materials such as cement and bentonite. Research are dealing both with the near-field environment - which is characterised by its evolution with time in terms of temperature, Eh balance, water composition - and the far-field environment, the chemistry of which is assumed to be roughly constant. Modelling the global RN migration in geological disposal requires having models which are intrinsically able to account for the evolution of the physical and chemical conditions of the environment. From the standpoint of performance assessment it is then necessary to acquire thermodynamic descriptions of the retention processes in order to perform calculations of the reactive transport of radionuclides In a first approach, CEA is developing for more than 15 years experiments and modelling to derive reliable predictive models for the RN migration in the geological disposal. For this purpose, a specific approach entitled the Ion-Exchangers Theory IXT was developed. It allows first to characterise the intrinsic retention properties of the pure minerals, i.e. to get evidence about the mono or multi-site character of the surface, to quantify the site(s) concentration(s) and to study the relative affinity of major solutes generally present in natural waters. This work provided a broad data

  5. Artrite da gota tofácea crônica mimetizando artrite reumatoide Chronic tophaceous gout mimicking rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Juliana F. Sarmento

    2009-12-01

    Full Text Available A gota é um distúrbio no metabolismo das purinas, usualmente associado à ocorrência de crises recorrentes de artrite nas articulações dos membros inferiores em homens entre 40-50 anos, e com o desenvolvimento de tofos subcutâneos nos pacientes com doença de longa evolução. Casos de pacientes com artrite gotosa crônica que mimetizam quadros de artrite reumatoide e vice-versa são raros. Descrevemos o caso de um paciente de 56 anos, com quadro de artrite poliarticular, simétrica e deformante, comprometendo principalmente as articulações de mãos e punhos, com nódulos subcutâneos difusos pelo corpo, alterações radiográficas atípicas e urolitíase, que, após avaliação clínica e dos exames complementares, recebeu diagnóstico de gota tofácea crônica mutilante mimetizando artrite reumatoide.Gout is a disorder of purine metabolism, usually associated with recurrent bouts of arthritis in the joints of the lower limbs, affecting men 40 to 50 years of age, which leads to the development of subcutaneous tophi in patients with long-lasting disease. Cases of patients with chronic gouty arthritis mimicking rheumatoid arthritis, and vice-versa, are rare. This report describes the case of a 56-year old male with symmetric, deforming, and polyarticular arthritis affecting, specially, the joints of the hands and wrists, with diffuse subcutaneous nodules throughout his body, atypical radiographic findings, and urolithiasis. Following clinical evaluation and additional tests, this patient received a diagnosis of chronic tophaceous gout mimicking mutilating rheumatoid arthritis.

  6. 肺癌抗原标志物联合检测的临床应用%Clinical application of joint detection with lung cancer antigen markers

    Institute of Scientific and Technical Information of China (English)

    温仁祝; 戴磊; 张亚男

    2015-01-01

    Objective To explore the best combination of joint detection with lung cancer antigen markers . Methods A random sample of 200 patients diagnosed with primary lung cancer patients ,measuring the level of a wide variety of tumor antigen markers in the blood and statistical analysis . Results In the detection of multiple tumor markers CEA ,NSE ,CYFRA21‐1 and CA125 positive rate is relatively high;serum markers in the expression of related to clinical stage . Conclusion The combined detection of CEA and CA125 ,NSE ,CYFRA21‐1 can im‐prove the accuracy of clinical diagnosis of lung cancer patients .%目的:探讨肺癌抗原标志物联合检测的最佳组合。方法随机抽取200例确诊为原发性肺癌的患者,测定患者血液中多种肿瘤抗原标志物的水平,并进行统计学分析。结果检测的多项肿瘤标志物中CEA、NSE、CYFRA21‐1和CA125的阳性率相对较高;血清各标志物的表达与临床分期有关。结论联合检测CEA、NSE、CYFRA21‐1和CA125能提高肺癌患者的临床诊断准确率。

  7. Tresyl-Based Conjugation of Protein Antigen to Lipid Nanoparticles Increases Antigen Immunogencity

    Science.gov (United States)

    Jain, Anekant; Yan, Weili; Miller, Keith R.; O'Carra, Ronan; Woodward, Jerold G.; Mumper, Russell J.

    2010-01-01

    The present studies were aimed at investigating the engineering of NPs with protein-conjugated-surfactant at their surface. In order to increase the immunogenicity of a protein antigen, Brij 78 was functionalized by tresyl chloride and then further reacted with the primary amine of the model proteins ovalbumin (OVA) or horseradish peroxide (HRP). The reaction yielded Brij 78-OVA and Brij 78-HRP conjugates which were then used directly to form NP-OVA or NP-HRP using a one-step warm oil-in-water microemulsion precursor method with emulsifying wax as the oil phase, and Brij 78 and the Brij 78-OVA or Brij 78-HRP conjugate as surfactants. Similarly, Brij 700 was conjugated to HIV p24 antigen to yield Brij 700-p24 conjugate. The utility of these NPs for enhancing the immune responses to protein-based vaccines was evaluated in vivo using ovalbumin (OVA) as model protein and p24 as a relevant HIV antigen. In separate in vivo studies, female BALB/c mice were immunized by subcutaneous (s.c.) injection with NP-OVA and NP-p24 formulations along with several control formulations. These results suggested that with multiple antigens, covalent attachment of the antigen to the NP significantly enhanced antigen-specific immune responses. This facile covalent conjugation and incorporation method may be utilized to further incorporate other protein antigens, even multiple antigens, into an enhanced vaccine delivery system. PMID:20837122

  8. Tresyl-based conjugation of protein antigen to lipid nanoparticles increases antigen immunogenicity.

    Science.gov (United States)

    Jain, Anekant; Yan, Weili; Miller, Keith R; O'Carra, Ronan; Woodward, Jerold G; Mumper, Russell J

    2010-11-30

    The present studies were aimed at investigating the engineering of NPs with protein-conjugated-surfactant at their surface. In order to increase the immunogenicity of a protein antigen, Brij 78 was functionalized by tresyl chloride and then further reacted with the primary amine of the model proteins ovalbumin (OVA) or horseradish peroxide (HRP). The reaction yielded Brij 78-OVA and Brij 78-HRP conjugates which were then used directly to form NP-OVA or NP-HRP using a one-step warm oil-in-water microemulsion precursor method with emulsifying wax as the oil phase, and Brij 78 and the Brij 78-OVA or Brij 78-HRP conjugate as surfactants. Similarly, Brij 700 was conjugated to HIV p24 antigen to yield Brij 700-p24 conjugate. The utility of these NPs for enhancing the immune responses to protein-based vaccines was evaluated in vivo using ovalbumin (OVA) as model protein and p24 as a relevant HIV antigen. In separate in vivo studies, female BALB/c mice were immunized by subcutaneous (s.c.) injection with NP-OVA and NP-p24 formulations along with several control formulations. These results suggested that with multiple antigens, covalent attachment of the antigen to the NP significantly enhanced antigen-specific immune responses. This facile covalent conjugation and incorporation method may be utilized to further incorporate other protein antigens, even multiple antigens, into an enhanced vaccine delivery system. PMID:20837122

  9. Near infra-red photoimmunotherapy with anti-CEA-IR700 results in extensive tumor lysis and a significant decrease in tumor burden in orthotopic mouse models of pancreatic cancer.

    Directory of Open Access Journals (Sweden)

    Ali A Maawy

    Full Text Available Photoimmunotherapy (PIT of cancer utilizes tumor-specific monoclonal antibodies conjugated to a photosensitizer phthalocyanine dye IR700 which becomes cytotoxic upon irradiation with near infrared light. In this study, we aimed to evaluate the efficacy of PIT on human pancreatic cancer cells in vitro and in vivo in an orthotopic nude mouse model. The binding capacity of anti-CEA antibody to BxPC-3 human pancreatic cancer cells was determined by FACS analysis. An in vitro cytotoxicity assay was used to determine cell death following treatment with PIT. For in vivo determination of PIT efficacy, nude mice were orthotopically implanted with BxPC-3 pancreatic tumors expressing green fluorescent protein (GFP. After tumor engraftment, the mice were divided into two groups: (1 treatment with anti-CEA-IR700 + 690 nm laser and (2 treatment with 690 nm laser only. Anti-CEA-IR700 (100 μg was administered to group (1 via tail vein injection 24 hours prior to therapy. Tumors were then surgically exposed and treated with phototherapy at an intensity of 150 mW/cm2 for 30 minutes. Whole body imaging was done subsequently for 5 weeks using an OV-100 small animal imaging system. Anti-CEA-IR700 antibody bound to the BxPC3 cells to a high degree as shown by FACS analysis. Anti-CEA-IR700 caused extensive cancer cell killing after light activation compared to control cells in cytotoxicity assays. In the orthotopic models of pancreatic cancer, the anti-CEA-IR700 group had significantly smaller tumors than the control after 5 weeks (p<0.001. There was no significant difference in the body weights of mice in the anti-CEA-IR700 and control groups indicating that PIT was well tolerated by the mice.

  10. Evaluation of the concrete shield compositions from the 2010 criticality accident alarm system benchmark experiments at the CEA Valduc SILENE facility

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Thomas Martin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Celik, Cihangir [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dunn, Michael E [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Wagner, John C [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); McMahan, Kimberly L [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Authier, Nicolas [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Jacquet, Xavier [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Rousseau, Guillaume [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Wolff, Herve [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Savanier, Laurence [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Baclet, Nathalie [French Atomic Energy Commission (CEA), Centre de Valduc, Is-sur-Tille (France); Lee, Yi-kang [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Trama, Jean-Christophe [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Masse, Veronique [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Gagnier, Emmanuel [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Naury, Sylvie [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Blanc-Tranchant, Patrick [French Atomic Energy Commission (CEA), Centre de Saclay, Gif sur Yvette (France); Hunter, Richard [Babcock International Group (United Kingdom); Kim, Soon [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Dulik, George Michael [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Reynolds, Kevin H. [Y-12 National Security Complex, Oak Ridge, TN (United States)

    2015-01-01

    In October 2010, a series of benchmark experiments were conducted at the French Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA) Valduc SILENE facility. These experiments were a joint effort between the United States Department of Energy Nuclear Criticality Safety Program and the CEA. The purpose of these experiments was to create three benchmarks for the verification and validation of radiation transport codes and evaluated nuclear data used in the analysis of criticality accident alarm systems. This series of experiments consisted of three single-pulsed experiments with the SILENE reactor. For the first experiment, the reactor was bare (unshielded), whereas in the second and third experiments, it was shielded by lead and polyethylene, respectively. The polyethylene shield of the third experiment had a cadmium liner on its internal and external surfaces, which vertically was located near the fuel region of SILENE. During each experiment, several neutron activation foils and thermoluminescent dosimeters (TLDs) were placed around the reactor. Nearly half of the foils and TLDs had additional high-density magnetite concrete, high-density barite concrete, standard concrete, and/or BoroBond shields. CEA Saclay provided all the concrete, and the US Y-12 National Security Complex provided the BoroBond. Measurement data from the experiments were published at the 2011 International Conference on Nuclear Criticality (ICNC 2011) and the 2013 Nuclear Criticality Safety Division (NCSD 2013) topical meeting. Preliminary computational results for the first experiment were presented in the ICNC 2011 paper, which showed poor agreement between the computational results and the measured values of the foils shielded by concrete. Recently the hydrogen content, boron content, and density of these concrete shields were further investigated within the constraints of the previously available data. New computational results for the first experiment are now available

  11. Changes of Serum Trace Elements, AFP, CEA, SF, T3, T4 and IGF- Ⅱ in Different Periods of Rat Liver Cancer

    Institute of Scientific and Technical Information of China (English)

    Hong-xu Zhang; Dan-dan Liu; Bai-jie Jin; Ya-wei Wang; Qj Liu; Ru-bing Duan; Peng Zhao; Ming-xia Ma

    2011-01-01

    Objective:Based on liver cancer model built in SD rats,the contents of trace elements (Cu,Fe,Zn,Ca and Mg),AFP,CEA,SF,TH and IGF-Ⅱ in serum were measured at different stages to explore the molecular changes during the rat liver cancer development.Methods:The SD rat liver cancer model was built by using diethylnitrosamine (DENA) as the mutagen.During 16 weeks of DENA gavage,blood samples were taken in the 14th,28th,56th,77th,105th and 112th days respectively after the first day of gavage with DENA,then the contents of five trace elements (Cu,Fe,Zn,Ca and Mg),T3,T4,IGF-Ⅱ,AFP,CEA and SF in serum were determined.Results:During the development of the rat liver cancer,in the test group,the Cu content significantly increased in serum,while the contents of Fe,Zn and Ca significantly decreased.The content of Mg showed no significant change.AFP and CEA of the test group showed same expression level with the control group; while the content of SF was lower than that of the control group when cancerization appeared.T3 and T4 increased at the first stage and then went down,and the content of IGF-Ⅱ was always high.Conclusion:Cu,Fe,Zn,Ca,T3,T4,SF and IGF-Ⅱ are closely related to the development of liver cancer.The changes of their contents in the development of cancer could enlighten the researches on cancer pathogenesis and prevention.

  12. Antigenic typing Polish isolates of canine parvovirus

    Energy Technology Data Exchange (ETDEWEB)

    Mizak, B. [National Veterinary Research Institute, Pulawy (Poland); Plucienniczak, A. [Polish Academy ofd Sciences. Microbiology and Virology Center, Lodz (Poland)

    1995-12-31

    Polish strains of canine parvovirus isolated between 1982 and 1993 were examined to determine the extent to which the virus has evolved antigenically and genetically over eleven years. Two CPV isolates obtained in Warsaw in 1982 and Pulawy in 1993, were examined using monoclonal antibody typing, restriction analysis and sequencing VP-2 protein gene. Five other isolates from Warsaw and Pulawy were tested with the panel of monoclonal antibodies specific to CPV-2, CPV-2a and common for canine parvovirus, feline panleukopenia virus and milk enteritis virus. Results of the studies demonstrated that all isolates tested represented CPV-2a antigenic type. Rapid antigenic strain replacement recorded by Parrish and Senda in the U.S.A and Japan was not confirmed in Poland. (author). 30 refs, 2 tabs.

  13. Antigen sampling in the fish intestine.

    Science.gov (United States)

    Løkka, Guro; Koppang, Erling Olaf

    2016-11-01

    Antigen uptake in the gastrointestinal tract may induce tolerance, lead to an immune response and also to infection. In mammals, most pathogens gain access to the host though the gastrointestinal tract, and in fish as well, this route seems to be of significant importance. The epithelial surface faces a considerable challenge, functioning both as a barrier towards the external milieu but simultaneously being the site of absorption of nutrients and fluids. The mechanisms allowing antigen uptake over the epithelial barrier play a central role for maintaining the intestinal homeostasis and regulate appropriate immune responses. Such uptake has been widely studied in mammals, but also in fish, a number of experiments have been reported, seeking to reveal cells and mechanisms involved in antigen sampling. In this paper, we review these studies in addition to addressing our current knowledge of the intestinal barrier in fish and its anatomical construction. PMID:26872546

  14. Idiopathic focal segmental glomerulosclerosis and HLA antigens.

    Science.gov (United States)

    Gerbase-DeLima, M; Pereira-Santos, A; Sesso, R; Temin, J; Aragão, E S; Ajzen, H

    1998-03-01

    The objective of the present study was to investigate a possible association between HLA class II antigens and idiopathic focal segmental glomerulosclerosis (FSGS). HLA-A, -B, -DR and -DQ antigens were determined in 19 Brazilian patients (16 white subjects and three subjects of Japanese origin) with biopsy-proven FSGS. Comparison of the HLA antigen frequencies between white patients and white local controls showed a significant increase in HLA-DR4 frequency among FSGS patients (37.7 vs 17.2%, P < 0.05). In addition, the three patients of Japanese extraction, not included in the statistical analysis, also presented HLA-DR4. In conclusion, our data confirm the association of FSGS with HLA-DR4 previously reported by others, thus providing further evidence for a role of genes of the HLA complex in the susceptibility to this disease. PMID:9698788

  15. Idiopathic focal segmental glomerulosclerosis and HLA antigens

    Directory of Open Access Journals (Sweden)

    M. Gerbase-DeLima

    1998-03-01

    Full Text Available The objective of the present study was to investigate a possible association between HLA class II antigens and idiopathic focal segmental glomerulosclerosis (FSGS. HLA-A, -B, -DR and -DQ antigens were determined in 19 Brazilian patients (16 white subjects and three subjects of Japanese origin with biopsy-proven FSGS. Comparison of the HLA antigen frequencies between white patients and white local controls showed a significant increase in HLA-DR4 frequency among FSGS patients (37.7 vs 17.2%, P<0.05. In addition, the three patients of Japanese extraction, not included in the statistical analysis, also presented HLA-DR4. In conclusion, our data confirm the association of FSGS with HLA-DR4 previously reported by others, thus providing further evidence for a role of genes of the HLA complex in the susceptibility to this disease

  16. Properties of glycolipid-enriched membrane rafts in antigen presentation.

    Science.gov (United States)

    Rodgers, William; Smith, Kenneth

    2005-01-01

    Presentation of antigen to T cells represents one of the central events in the engagement of the immune system toward the defense of the host against pathogens. Accordingly, understanding the mechanisms by which antigen presentation occurs is critical toward our understanding the properties of host defense against foreign antigen, as well as insight into other features of the immune system, such as autoimmune disease. The entire antigen-presentation event is complex, and many features of it remain poorly understood. However, recent studies have provided evidence showing that glycolipid-enriched membrane rafts are important for efficient antigen presentation; the studies suggest that one such function of rafts is trafficking of antigen-MHC II complexes to the presentation site on the surface of the antigen-presenting cell. Here, we present a critical discussion of rafts and their proposed functions in antigen presentation. Emerging topics of rafts and antigen presentation that warrant further investigation are also highlighted.

  17. Vegetación espontánea preferida por hormigas cortadoras en plantaciones de Salicáceas en el Delta del Paraná

    OpenAIRE

    Perri, Daiana; Casaubón, Edgardo; Gorosito, Norma; Gurini, Laura Beatriz; Fernández, Patricia Carina

    2015-01-01

    La vegetación de crecimiento espontáneo, llamada “maleza” es considerada perjudicial para las plantaciones de sauce y álamo. Las hormigas cortadoras de hojas (Acromyrmex sp.) son una de las plagas más importantes en la producción de Salicáceas y son combatidas con insecticidas de síntesis que contaminan el ambiente. El objetivo de este trabajo fue comenzar a evaluar las plantas cortadas por las hormigas en el campo y realizar ensayos de preferencia con el fin de proponer especies como cultivo...

  18. Diagnostic value of Cyfra21-1, SCC and CEA for differentiation of early-stage NSCLC from benign lung disease

    OpenAIRE

    Chen, Feng; Wang, Xiu-Ying; Han, Xiao-Hong; Wang, Hai; Qi, Jun

    2015-01-01

    Non-small cell lung cancer (NSCLC), which account for the most of lung carcinoma, is sometimes difficult to differentiate from benign lung diseases presented with nodular shadow in imaging scan. There is a need to find another non-invasive way to diagnosis early-stage NSCLC. To examine the potential diagnostic value of SCC, CFYRA 21-1 and CEA for the differentiation of early-stage NCSCL from benign lung diseases, we analyzed serum levels of tumor markers in 278 patients, including 248 patient...

  19. Preoperative CYFRA 21-1 and CEA as Prognostic Factors in Patients with Stage I Non-Small Cell Lung Cancer

    OpenAIRE

    Blankenburg, Florian; Hatz, Rudolf; Nagel, Dorothea; Ankerst, Donna Pauler; Reinmiedl, Judith; Gruber, Christine; Seidel, Dietrich; Stieber, Petra

    2008-01-01

    Objective: To validate the prognostic value of preoperative levels of CYFRA 21-1, CEA and the corresponding tumor marker index (TMI) in patients with stage I non-small cell lung cancer (NSCLC). Methods: Two hundred forty stage I NSCLC patients (80 in pT1 and 160 in pT2; 100 squamous cell carcinomas, 91 adenocarcinomas, 32 large-cell carcinomas, 17 with other histologies; 171 males and 69 females) who had complete resection (R0) between 1986 and 2004 were included in the analysis. CYFRA 21-1 a...

  20. Serum tetranectin is an independent prognostic marker in colorectal cancer and weakly correlated with plasma suPAR, plasma PAI-1 and serum CEA

    DEFF Research Database (Denmark)

    Høgdall, Claus K; Christensen, Ib J; Stephens, Ross W;

    2002-01-01

    to have an independent prognostic value for survival (log TN: HR = 0.47, 95% CI: 0.29-0.76); log soluble uPAR: HR = 1.65, 95% CI: 1.18-2.31; log CEA: HR = 1.I1, 95% CI: 1.03-1.20). Based on the multivariate model, a patient with a combination of low levels of TN and PAI-1 and elevated levels of...... prognostic factor in patients with colorectal cancer. TN may be valuable as a prognostic variable in future studies evaluating new treatment strategies for colorectal cancer....

  1. Techniques and devices developed by the CEA for hot cell and in-situ examinations of PWR components and PWR fuel assembliess after irradiation

    International Nuclear Information System (INIS)

    Within the framework of the electro-nuclear development of the PWR system, the CEA has provided itself with facilities for developing techniques for analyzing assemblies, pins and fuels. These are examinations and tests on irradiated heads and assemblies with the aid of the Fuel Examination Module (FEM), of machining of assemblies and examinations in the Celimene hot laboratory or detailed examinations and analyses on fuel elements using eddy currents, the electronic microprobe and the Fisher ''permeascope'' which enables the outline of the oxide coat present on the cladding to be followed

  2. Flow modelling in fractured aquifers, development of multi-continua model (direct and inverse problems) and application to the CEA/Cadarache site

    International Nuclear Information System (INIS)

    This research thesis concerns the modelling of aquifer flows under the CEA/Cadarache site. The author reports the implementation of a numerical simulation tool adapted to large scale flows in fractured media, and its application to the Cadarache nuclear site. After a description of the site geological and hydrogeological characteristics, the author presents the conceptual model on which the modelling is based, presents the inverse model which allows a better definition of parameters, reports the validation of the inverse approach by means of synthetic and semi-synthetic cases. Then, he reports experiments and simulation of the Cadarache site

  3. Produção de mudas de cucurbitáceas utilizando esterco ovino na composição de substratos orgânicos.

    OpenAIRE

    Souza, Enio Gomes Flôr; Santana, Falkner Michael de Sousa; Martins, Bruno Novaes Menezes; Barros Júnior, Aurélio Paes; Silveira, Lindomar Maria da; Pereira, Daniel Lima

    2014-01-01

    Objetivou-se avaliar a emergência e desenvolvimento de mudas de cucurbitáceas em substratos à base de esterco ovino. Foram realizados três experimentos entre novembro de 2010 e abril de 2011, em Serra Talhada-PE. Foi utilizado o delineamento experimental inteiramente casualizado, com quatro repetições. Os tratamentos foram compostos por 10 substratos: substrato comercial (Tropstrato HT®); esterco ovino + areia (1:1); esterco ovino + areia (2:1); esterco ovino + areia (3:1); esterco ovino +...

  4. List of reports in the field of reactor safety research from BMFT, CEA, EPRI, JSTA and USNRC. Reported period: July 1 to December 31, 1993

    International Nuclear Information System (INIS)

    This list reviews reports from the Federal Republic of Germany, from France, from Japan and from the United States of America concerning single problems in the field of Reactor Safety Research. According to the cooperation of the Bundesministerium fuer Forschung und Technologie (BMFT) with the Commissariat a l'Energie Atomique (CEA), the Japan Science and Technologie Agency (ISTA), the Electric Power Research Institute (EPRI) and the United States Nuclear Regulatory Commission, these reports are available in the Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS). The list pursues the following order: Country of origin, problem area concerned, according to the Reactor Safety Research Program of the BMFT, reporting organization. (orig./HP)

  5. List of reports in the field of reactor safety research from BMFT, CEA, EPRI, JSTA and USNRC. Reported period: January 1 to June 30, 1994

    International Nuclear Information System (INIS)

    This list reviews reports from the Federal Republic of Germany, from France, from Japan and from the United States of America concerning single problems in the field of Reactor Safety Research. According to the cooperation of the Bundesministerium fuer Forschung und Technologie (BMFT) with the Commissariat a l'Energie Atomique (CEA), the Japan Science and Technologie Agency (ISTA), the Electric Power Research Institute (EPRI) and the United States Nuclear Regulatory Commission, these reports are available in the Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS). The list pursues the following order: Country of origin, problem area concerned, according to the Reactor Safety Research Program of the BMFT, reporting organization. (orig./HP)

  6. Antigen processing and remodeling of the endosomal pathway: requirements for antigen cross-presentation.

    Directory of Open Access Journals (Sweden)

    Ewoud Bernardus Compeer

    2012-03-01

    Full Text Available The cross-presentation of endocytosed antigen as peptide/class I MHC complexes plays a central role in the elicitation of CD8+ T cell clones that mediate anti-viral and anti-tumor immune responses. While it has been clear that there are specific subsets of professional antigen presenting cells (APC capable of antigen cross-presentation, description of mechanisms involved is still ongoing. Especially amongst dendritic cells (DC, there are specialized subsets that are highly proficient at antigen cross-presentation. We here present a focused survey on the cell biological processes in the endosomal pathway that support antigen cross-presentation. This review highlight DC-intrinsic mechanisms that facilitate the cross-presentation of endocytosed antigen, including receptor-mediated uptake, recycling and maturation including the sorting of membrane proteins, dynamic remodeling of endosomal structures and cell-surface directed endosomal trafficking. We will conclude with description of pathogen-induced deviation of endosomal processing, and discuss how immune evasion strategies pertaining endosomal trafficking may preclude antigen cross-presentation.

  7. Antigen-Antibody Interaction Database (AgAbDb): a compendium of antigen-antibody interactions.

    Science.gov (United States)

    Kulkarni-Kale, Urmila; Raskar-Renuse, Snehal; Natekar-Kalantre, Girija; Saxena, Smita A

    2014-01-01

    Antigen-Antibody Interaction Database (AgAbDb) is an immunoinformatics resource developed at the Bioinformatics Centre, University of Pune, and is available online at http://bioinfo.net.in/AgAbDb.htm. Antigen-antibody interactions are a special class of protein-protein interactions that are characterized by high affinity and strict specificity of antibodies towards their antigens. Several co-crystal structures of antigen-antibody complexes have been solved and are available in the Protein Data Bank (PDB). AgAbDb is a derived knowledgebase developed with an objective to compile, curate, and analyze determinants of interactions between the respective antigen-antibody molecules. AgAbDb lists not only the residues of binding sites of antigens and antibodies, but also interacting residue pairs. It also helps in the identification of interacting residues and buried residues that constitute antibody-binding sites of protein and peptide antigens. The Antigen-Antibody Interaction Finder (AAIF), a program developed in-house, is used to compile the molecular interactions, viz. van der Waals interactions, salt bridges, and hydrogen bonds. A module for curating water-mediated interactions has also been developed. In addition, various residue-level features, viz. accessible surface area, data on epitope segment, and secondary structural state of binding site residues, are also compiled. Apart from the PDB numbering, Wu-Kabat numbering and explicit definitions of complementarity-determining regions are provided for residues of antibodies. The molecular interactions can be visualized using the program Jmol. AgAbDb can be used as a benchmark dataset to validate algorithms for prediction of B-cell epitopes. It can as well be used to improve accuracy of existing algorithms and to design new algorithms. AgAbDb can also be used to design mimotopes representing antigens as well as aid in designing processes leading to humanization of antibodies. PMID:25048123

  8. [Presence of Australia antigen in blood donors].

    Science.gov (United States)

    Gota, F

    1980-01-01

    The differential diagnosis of type A and B viral hepatitis is discussed and guidelines for the prevention of post-transfusional hospital hepatitis are proposed. Methods for the immunological demonstration of HBs antigen are illustrated, together with the respective positivity percentages in blood donors.

  9. HLA antigens and asthma in Greeks.

    Science.gov (United States)

    Apostolakis, J; Toumbis, M; Konstantopoulos, K; Kamaroulias, D; Anagnostakis, J; Georgoulias, V; Fessas, P; Zervas, J

    1996-04-01

    HLA-A and -B antigens were determined in a group of 76 Greek asthmatic patients: 35 children (1.5-15 years) and 41 adults (18-73 years). The results were compared to those of 400 healthy unrelated controls from the same population. The standard NIH lymphocytotoxicity test was applied. When all 76 patients were compared to the controls, a statistically significant lower frequency of HLA-B5 and -B35 antigens was noted. When adults were analysed alone, an increased frequency of HLA-B8 was found. On the other hand, in the asthmatic children sub-group, the HLA-A10 antigen was significantly higher and the HLA-B5 was significantly lower than in the controls. These data imply that different HLA antigens may be involved in the pathogenesis of several clinical forms of asthma and that, in order to study the role of immunogenetic factor(s) in the pathogenesis of this disease, more adequate grouping criteria are needed.

  10. Antigen dynamics of follicular dendritic cells

    NARCIS (Netherlands)

    Heesters, B.A.

    2015-01-01

    Stromal-derived follicular dendritic cells (FDCs) are a major depot for antigen that are essential for formation of germinal centers, the site where memory and effector B cells differentiate and high-affinity antibody production takes place. Historically, FDCs have been characterized as ‘accessory’

  11. Circulating filarial antigen detection in brugian filariasis.

    Science.gov (United States)

    Tripathi, Praveen Kumar; Mahajan, Ramesh Chander; Malla, Nancy; Mewara, Abhishek; Bhattacharya, Shailja Misra; Shenoy, Ranganatha Krishna; Sehgal, Rakesh

    2016-03-01

    Human lymphatic filariasis (LF) is a major cause of disability globally. The success of global elimination programmes for LF depends upon effectiveness of tools for diagnosis and treatment. In this study on stage-specific antigen detection in brugian filariasis, L3, adult worm (AW) and microfilarial antigenaemia were detected in around 90-95% of microfilariae carriers (MF group), 50-70% of adenolymphangitis (ADL) patients, 10-25% of chronic pathology (CP) patients and 10-15% of endemic normal (EN) controls. The sensitivity of the circulating filarial antigen (CFA) detection in serum samples from MF group was up to 95%. In sera from ADL patients, unexpectedly, less antigen reactivity was observed. In CP group all the CFA positive individuals were from CP grade I and II only and none from grade III or IV, suggesting that with chronicity the AWs lose fecundity and start to disintegrate and die. Amongst EN subject, 10-15% had CFA indicating that few of them harbour filarial AWs, thus they might not be truly immune as has been conventionally believed. The specificity for antigen detection was 100% when tested with sera from various other protozoan and non-filarial helminthic infections.

  12. Wegener's granulomatosis and autoantibodies to neutrophil antigens

    OpenAIRE

    McCluskey, D R; Maxwell, A. P.; Watt, L

    1988-01-01

    We report five cases of Wegener's granulomatosis all of whom had clinical and histological evidence of disease activity at presentation and in whom autoantibodies to neutrophil antigens were detected. This test may prove useful for the diagnosis of this serious condition and help to monitor disease activity during treatment.

  13. Antigenic characterisation of lyssaviruses in South Africa

    Directory of Open Access Journals (Sweden)

    Ernest Ngoepe

    2014-02-01

    Full Text Available There are at least six Lyssavirus species that have been isolated in Africa, which include classical rabies virus, Lagos bat virus, Mokola virus, Duvenhage virus, Shimoni bat virus and Ikoma lyssavirus. In this retrospective study, an analysis of the antigenic reactivity patterns of lyssaviruses in South Africa against a panel of 15 anti-nucleoprotein monoclonal antibodies was undertaken. A total of 624 brain specimens, collected between 2005 and 2009, confirmed as containing lyssavirus antigen by direct fluorescent antibody test, were subjected to antigenic differentiation. The lyssaviruses were differentiated into two species, namely rabies virus (99.5% and Mokola virus (0.5%. Furthermore, rabies virus was further delineated into two common rabies biotypes in South Africa: canid and mongoose. Initially, it was found that the canid rabies biotype had two reactivity patterns; differential staining was observed with just one monoclonal antibody. This difference was likely to have been an artefact related to sample quality, as passage in cell culture restored staining. Mongoose rabies viruses were more heterogeneous, with seven antigenic reactivity patterns detected. Although Mokola viruses were identified in this study, prevalence and reservoir host species are yet to be established. These data demonstrate the usefulness of monoclonal antibody typing panels in lyssavirus surveillance with reference to emergence of new species or spread of rabies biotypes to new geographic zones.

  14. Lea blood group antigen on human platelets

    International Nuclear Information System (INIS)

    One- and two-stage radioligand assays were used to determine if human platelets possess the Lea antigen. Goat IgG anti-Lea antibody was purified by multiple adsorptions with Le(a-b-) human red blood cells, followed by affinity chromatography with synthetic Lea substance and labeling with 125I. Human IgG anti-Lea antibody was used either in a two stage radioassay with 125I-labeled mouse monoclonal IgG anti-human IgG as the second antibody or, alternatively, purified by Staph protein A chromatography, labeled with 125I, and used in a one-stage radioassay. Platelets from donors of appropriate red blood cell phenotypes were incubated with the antisera, centrifuged through phthalate esters, and assayed in a gamma scintillation counter. Dose response and saturation curve analysis demonstrate the presence of Lewis a antigen on platelets from Lea+ donors. Furthermore, platelets from an Le(a-b-) donor incubated in Le (a+b-) plasma adsorb Lea antigen in a similar manner to red blood cells. The clinical significance of these antigens in platelet transfusion remains undefined

  15. LTA ,CEA,NSE,Cyfra 21-1 对肺癌诊断价值的探讨%Diagnostic Value of Non-small-cell Lung tumor Antigen (LTA),Carcinoembryonic Anitigen (CEA), Neuron-specific Enolase (NSE)and Cytokeratin Fragment 19(Cyfra21-1) in Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    周彩存; 谈立松

    2001-01-01

    目的探讨LTA、CEA、NSE和Cyfra21-1对肺癌诊断价值.方法清晨空腹抽取临床确诊肺癌患者124例、转移性肺癌9例和良性肺部疾病34例患者静脉血7ml,分别用放射免疫法和乳胶凝集试验法,检测血清中上述癌性标志物水平.结果:LTA、CEA、NSE和Cyfra21-1,在肺癌的阳性率分别为60%、8%、31%和43%;特异性分别为88%、100%、84%和97%,CEA和Cyfra21-1较高;阳性率和敏感性组间差异有统计学意义.LTA对肺癌细胞类型和是否手术无诊断意义.结论LTA对肺癌具有一定的诊断价值,但特异性差于Cyfra21-1;多种癌性标志物同时检测有助提高阳性率.

  16. ACCELERATED AGING OF OLERACEOUS SPECIES SEEDS ENVELHECIMENTO ACELERADO DE SEMENTES DE ESPÉCIES OLERÁCEAS

    Directory of Open Access Journals (Sweden)

    Denis Santiago da Costa

    2011-07-01

    the seedling formation process.

    A qualidade da semente utilizada no processo de produção agrícola é um dos principais fatores considerados para a implantação da cultura. Este trabalho objetivou estudar o efeito do nível de vigor no desempenho de sementes de seis espécies oleráceas. Sementes comerciais de alface, berinjela, brócolis, couve-flor, rúcula e tomate foram submetidas ao envelhecimento acelerado, por 48 e 72 horas, para obtenção de lotes com três níveis de vigor. Obteve-se um lote normal e dois envelhecidos por diferentes períodos. Após a obtenção dos lotes, as sementes foram submetidas a testes de germinação, condutividade elétrica, emergência de plântulas em bandeja, comprimento e massa seca de plântulas. O efeito do envelhecimento acelerado torna-se mais acentuado com o aumento do tempo de exposição das sementes às condições de alta umidade relativa do ar e elevada temperatura. Esta exposição reduz, mais acentuadamente, a qualidade das sementes para algumas espécies, como alface, couve-flor e rúcula, que para outras, como brócolis, tomate e berinjela. Sementes de menor vigor, além de menor germinação e menor emergência, apresentam lentidão no processo de formação de mudas.

  17. Research on immunosensor for the determination of carcinoembryonic antigen and its application%免疫传感器在癌胚抗原检测中的构建与应用研究

    Institute of Scientific and Technical Information of China (English)

    马霄; 莫利明; 虞成; 逯岭松; 杨攀

    2014-01-01

    目的:设计一种新型的基于丝网印刷技术的癌胚抗原(CEA)免疫传感器,用于癌胚抗原浓度的床边检测。方法:在丝网印刷电极(SPE)上层层修饰上多壁碳纳米管(MWNTs)N,N-二甲基甲酰胺(DMF)分散液与壳聚糖纳米二氧化铈(Chitosan-Nano CeO2)溶液两种纳米材料,利用纳米铈固载癌胚抗原抗体(anti-CEA),最后用牛血清白蛋白(BSA)封闭纳米铈上非特异位点,构建一种新型的癌胚抗原免疫传感器。采用透射电镜(TEM)及循环伏安法(CV)对修饰过程进行表征。结果:探讨不同pH、孵育温度及孵育时间对该免疫传感器性能的影响,该传感器在最适条件下对CEA响应良好,其线性范围为0~80 ng/ml,线性相关系数r=0.99825,检出限为0.08 ng/ml。结论:CEA免疫传感器具有稳定性好、灵敏度高、特异性好、结果准确可靠和可再生等优点,可应用于临床检测。%Objective: To design immunosensor for the determination of Carcinoembryonic Antigen(CEA) and be used in the piont-of-care test of the concentration of CEA. Methods:The immunosensor was developed by modifying multi-wall carbon nanotubes(MWNTs)which dispersed in N, N-dimethyl-formamide(DMF)on the Screen-Printed Electrodes (SPE), then Chitosan-NanoCeO2 were immobilized self-assembly. And then the antibody of CEA (anti-CEA) was absorbed on the surface of NanoCeO2 monolayer. Finally, Albumin from bovine serum (BSA) blocked the non-specific adsorption site.The modification process was characterized by transmisCeOn electronmicroscope(TEM)and cyclic voltammetry(CV). The factors possibly influenced the performance of the proposed immunosensor were studied in detail. Results:Under optimal conditions,the obtained immunosensor exhibited good electrochemical behavior to CEA in a linear range of 0 to 80 ng/ml (r=0.99825), with a detection limit of 0.08 ng/ml. Conclusion: The immunosensor prepared using this method has advantages of good

  18. Cysteine proteases as potential antigens in antiparasitic DNA vaccines

    DEFF Research Database (Denmark)

    Jørgensen, Louise von Gersdorff; Buchmann, Kurt

    2011-01-01

    En litteraturgennemgang af muligheder for at bruge cystein proteaser som antigener i antiparasitære vacciner.......En litteraturgennemgang af muligheder for at bruge cystein proteaser som antigener i antiparasitære vacciner....

  19. Dengue viruses cluster antigenically but not as discrete serotypes

    NARCIS (Netherlands)

    L. Katzelnick (Leah); J.M. Fonville (Judith); G.D. Gromowski (Gregory D.); J.B. Arriaga (Jose Bustos); A. Green (Angela); S.L. James (Sarah ); L. Lau (Louis); M. Montoya (Magelda); C. Wang (Chunling); L.A. Van Blargan (Laura A.); C.A. Russell (Colin); H.M. Thu (Hlaing Myat); T.C. Pierson (Theodore C.); P. Buchy (Philippe); J.G. Aaskov (John G.); J.L. Muñoz-Jordán (Jorge L.); N. Vasilakis (Nikos); R.V. Gibbons (Robert V.); R.B. Tesh (Robert B.); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron); A. Durbin (Anna); C.P. Simmons (Cameron P.); E.C. Holmes (Edward C.); E. Harris (Eva); S.S. Whitehead (Stephen S.); D.R. Smith (Derek Richard)

    2015-01-01

    textabstractThe four genetically divergent dengue virus (DENV) types are traditionally classified as serotypes. Antigenic and genetic differences among the DENV types influence disease outcome, vaccine-induced protection, epidemic magnitude, and viral evolution.We scharacterized antigenic diversity

  20. Uso da capilaroscopia ungueal como método diagnóstico e prognóstico de rosácea Nailfold capillaroscopy as a diagnostic and prognostic method in rosacea

    OpenAIRE

    Gabriela Poglia Fonseca; Fabiane Mulinari Brenner; Carolina de Souza Muller; Adma Lima Wojcik

    2011-01-01

    FUNDAMENTOS: Não há um método adequado e fidedigno de avaliação e seguimento da severidade na rosácea. OBJETIVO: Determinar a importância da capilaroscopia periungueal como método diagnóstico e prognóstico em pacientes portadores de rosácea. MÉTODOS: Estudo transversal onde foram submetidos ao exame da capilaroscopia periungueal 8 pacientes com rosácea e 8 controles no período entre maio e julho de 2009. Foram coletados dados clínicos relacionados ao sexo, idade, fototipo, classificação da ro...