WorldWideScience

Sample records for causadas por candida

  1. Ptose palpebral causada por Paquidermoperiostose

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    Patricia Regina de Pinho Tavares

    2014-08-01

    Full Text Available A paquidermoperiostose é uma síndrome caracterizada por acometimento cutâneo e ósseo, e em alguns casos ocorre comprometimento palpebral leve. É uma síndrome rara, idiopática ou hereditária, com provável herança autossômica dominante de penetrância variável. Descreve-se o caso de um paciente com ptose grave por paquidermoperiostose elucidando sua fisiopatologia e conduta cirúrgica aplicada.

  2. Muerte cerebral causada por consumo de psicofármacos

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    Iglesias Rozas, José Rafael, 1942-

    1987-01-01

    Tres imágenes de una biopsia de un cerebro en estado de muerte cerebral causada por consumo de psicofármacos. Three pictures of a biopsy of a brain that has died because of consumption of psychoactive drugs.

  3. Endoftalmite por Candida albicans Candida albicans endophthalmitis

    OpenAIRE

    Pedro Duraes Serracarbassa; Patrícia Dotto

    2003-01-01

    O autor descreve os aspectos epidemiológicos, histopatológicos e clínicos da endoftalmite endógena por Candida albicans. Apresenta ainda novos métodos diagnósticos e opções terapêuticas utilizadas no tratamento das infecções fúngicas intra-oculares, por meio de revisão bibliográfica.The author describes epidemiological, histopathological and clinical aspects of endogenous Candida albicans endophthalmitis. He also presents new diagnostic methods and therapeutical options to treat intraocular f...

  4. Endoftalmite por Candida albicans Candida albicans endophthalmitis

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    Pedro Duraes Serracarbassa

    2003-10-01

    Full Text Available O autor descreve os aspectos epidemiológicos, histopatológicos e clínicos da endoftalmite endógena por Candida albicans. Apresenta ainda novos métodos diagnósticos e opções terapêuticas utilizadas no tratamento das infecções fúngicas intra-oculares, por meio de revisão bibliográfica.The author describes epidemiological, histopathological and clinical aspects of endogenous Candida albicans endophthalmitis. He also presents new diagnostic methods and therapeutical options to treat intraocular fungal infections, based on literature review.

  5. Mancha areolada causada por Thanatephorus cucumeris em mogno africano

    OpenAIRE

    GAPAROTTO LUADIR; HANADA ROGÉRIO E.; ALBUQUERQUE FERNANDO C.; DUARTE MARIA DE LOURDES R.

    2001-01-01

    O mogno africano (Khaya ivorensis) foi introduzido no Brasil para substituir o mogno brasileiro (Swietenia macrophylla) devido a sua alta resistência ao microlepidóptero Hypsiphyla grandella, a principal praga do mogno brasileiro. Em 1999, observou-se alta incidência da mancha areolada, causada por Thanatephorus cucumeris em viveiros e áreas de plantios definitivos de mogno africano nos Estados do Amazonas e Pará, causando lesões em folhas maduras e 100% de queda das folhas jovens. Nas folhas...

  6. Neuropatia Óptica Tóxica causada por Dissulfiram

    OpenAIRE

    Lemos, José Alberto; Ribeiro, Isabel; Martins, João; Menezes, Carlos; Gonçalves, Rita; Coelho, Pedro; Maio, Tiago

    2015-01-01

    Objetivos: O dissulfiram é utilizado no tratamento do alcoolismo crónico há mais de 50 anos. Este fármaco interfere no metabolismo do etanol e encoraja a abstinência por causar sintomas desagradáveis com a ingestão concomitante de álcool. Efeitos adversos incluem casos raros de neuropatia óptica bilateral. O objetivo deste artigo é descrever um caso clínico de neuropatia óptica tóxica causada por dissulfiram. Métodos: Descrição do caso clínico e breve revisão bibliográfica. Resultados: Report...

  7. Tratamiento con caspofungina de endocarditis por Candida tropicalis resistente a fluconazol Treatment with caspofungin of Candida tropicalis endocarditis resistant to fluconazol

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    Marcelo del Castillo

    2004-04-01

    Full Text Available Las endocarditis causadas por hongos, (Candida en particular, requieren tratamiento médico-quirúrgico, siendo la anfotericina B la droga de elección. Caspofungina es una equinocandina con gran actividad sobre Candida y Aspergillus. Se presenta un paciente con una endocarditis por Candida tropicalis resistente a fluconazol tratado con caspofungina bajo un esquema de salvataje, luego de haber presentado efectos adversos por anfotericina B. El paciente tuvo respuesta microbiológica.Fungal endocarditis, in particular due to Candida species, requires medical and surgical treatment and amphotericin B is the drug of choice. Caspofungin is an echinocandin very effective against Candida and Aspergillus. We present a patient with Candida tropicalis endocarditis, fluconazol resistant, treated with caspofungin, on a compassional basis as a result of adverse effects with amphotericin B. The patient had a microbiological response.

  8. Síndrome oculoglandular de Parinaud causada por esporotricose Parinaud's oculoglandular syndrome caused by Sporotrichosis

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    Alexandre Sampaio de Abreu Ribeiro; Tiago Bisol; Marcela Sant'Ana Menezes

    2010-01-01

    A síndrome oculoglandular de Parinaud é uma doença ocular rara causada por diferentes agentes etiológicos, entre eles bactérias, vírus e fungos. É caracterizada por uma conjuntivite granulomatosa, acompanhada de linfadenopatia pré-auricular adjacente e pode trazer sequelas caso não seja prontamente tratada. Neste artigo é relatado o caso de uma jovem técnica de enfermagem e estudante de medicina veterinária apresentando a síndrome oculoglandular de Parinaud causada pelo fungo Sporothrix schen...

  9. Candida albicans skin abscess Abscesso de pele por Candida albicans

    OpenAIRE

    Felipe Francisco Tuon; Antonio Carlos Nicodemo

    2006-01-01

    Subcutaneous candidal abscess is a very rare infection even in immunocompromised patients. Some cases are reported when breakdown in the skin occurs, as bacterial cellulites or abscess, iatrogenic procedures, trauma and parenteral substance abuse. We describe a case of Candida albicans subcutaneous abscess without fungemia, which can be associated with central venous catheter.Abscesso subcutâneo por Candida é infecção muito rara mesmo em pacientes imunocomprometidos. Alguns casos são relatado...

  10. Candida albicans skin abscess Abscesso de pele por Candida albicans

    Directory of Open Access Journals (Sweden)

    Felipe Francisco Tuon

    2006-10-01

    Full Text Available Subcutaneous candidal abscess is a very rare infection even in immunocompromised patients. Some cases are reported when breakdown in the skin occurs, as bacterial cellulites or abscess, iatrogenic procedures, trauma and parenteral substance abuse. We describe a case of Candida albicans subcutaneous abscess without fungemia, which can be associated with central venous catheter.Abscesso subcutâneo por Candida é infecção muito rara mesmo em pacientes imunocomprometidos. Alguns casos são relatados quando ocorre dano na pele, como celulite bacteriana ou abscesso, procedimentos iatrogênicos, trauma e abuso de substância parenteral. Relatamos caso de abscesso subcutâneo por Candida albicans sem fungemia, que pode estar associado com cateter venoso central.

  11. Danos na soja causada por míldio

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    Olavo Correia da Silva

    2016-03-01

    Full Text Available RESUMO: O míldio da soja é disseminado em todo mundo. Entretanto, o dano que ele causa não tem sido estudado no Brasil. O objetivo deste trabalho foi avaliar quais componentes de rendimento da soja são afetados por essa doença e determinar o coeficiente de dano. Dois experimentos foram conduzidos, um em Castro (2006/07 e outro em Ponta Grossa (2007/08, no Estado do Paraná, Brasil. O delineamento experimental foi de blocos ao acaso com (i seis tratamentos e cinco repetições para os experimentos de campo, safras 2006/07, e (ii oito tratamentos e quatro repetições para 2007/08. Foram aplicados fosfito de potássio (750g ia ha-1, propamocarb + fenamidona (900g ia ha-1 e mancozeb (2,400g ia ha-1. Em todos os tratamentos, a severidade máxima da infecção pelo míldio ocorreu no estádio R5.3 e a porcentagem de severidade variou entre 0 e 43%. O gradiente da doença foi obtido apenas na segunda safra. Equações de danos foram geradas para o peso de grãos e produtividade. O número de grãos por vagem e o número de vagens por planta não foram afetados pelo míldio mas o peso de 1000 sementes por planta foi reduzido linearmente com o aumento da severidade do míldio.

  12. Resistencia y control químico en la Antracnosis del olivo causada por Colletotrichum spp.

    OpenAIRE

    Xavier, Carlos José

    2015-01-01

    La Antracnosis o Aceituna Jabonosa, es la enfermedad más importante del fruto del olivo y origina pérdidas estimadas en unos 75 millones de euros en España. La Antracnosis está causada por varias especies de hongos del género Colletotrichum pertenecientes a los complejos Colletotrichum acutatum sensu lato (s.l) y Colleotrichum gloesoporiodes s.l. En Andalucía, las especies más frecuentes asociadas con la Antracnosis pertenecen al complejo C. acutatum s.l. La enfermedad presenta...

  13. Tratamento laparoscópico de periapendicite causada por dispositivo intra-uterino

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    Coelho Júlio Cezar Uili

    2003-01-01

    Full Text Available RACIONAL: Dispositivo intra-uterino pode perfurar o útero e causar várias complicações. OBJETIVO: Descrever um caso de tratamento laparoscópico de periapendicite causada por dispositivo intra-uterino. MATERIAL E MÉTODOS: Jovem do sexo feminino apresentou dor em fossa ilíaca direita de 4 dias de duração. No exame físico, apresentava dor abdominal localizada em fossa ilíaca direita, com discreta renitência de parede e dor à percussão e descompressão brusca na região. A ultra-sonografia evidenciou dispositivo intra-uterino fora do útero, na fossa ilíaca direita. RESULTADOS: À laparoscopia, observou-se massa inflamatória que consistia do dispositivo intra-uterino totalmente bloqueado pelo mesoapêndice e apêndice. Apendicectomia e retirada do dispositivo foram realizadas. CONCLUSÃO: A laparoscopia permite o tratamento adequado da periapendicite causada por dispositivo intra-uterino, inclusive com avaliação adequada de toda a região pélvica para excluir lesões associadas.

  14. Mastite micótica em ruminantes causada por leveduras Mycotic mastitis in ruminants caused by yeasts

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    Andréia Spanamberg

    2009-02-01

    Full Text Available A ocorrência de casos esporádicos de mastite causados por microrganismos de origem ambiental pode ser considerada como emergente e as leveduras, os fungos leveduriformes e os filamentosos são alguns dos principais agentes envolvidos. Em relação à mastite micótica, as leveduras são freqüentemente causas de infecções da glândula mamária em animais produtores de leite. A maior parte dos casos ocorre sob a forma de surtos localizados e/ou após tratamento com antimicrobianos. Os principais gêneros envolvidos são Candida e Cryptococcus, além de outros como Geotrichum, Pichia e Trichosporon. O objetivo do trabalho é revisar os aspectos gerais sobre a mastite micótica causada por leveduras e fungos leveduriformes, tais como etiologia, causas predisponentes, patogenicidade, diagnóstico, tratamento e profilaxia por meio de uma abordagem cronológica dos primeiros relatos, mas com enfoque principal nos resultados descritos recentemente na literatura veterinária nacional e internacional. O controle da mastite micótica é fundamentado em métodos preventivos, como adequado manejo da ordenha, correta higienização dos equipamentos e das instalações, com a finalidade de reduzir o número de animais acometidos, além de garantir a qualidade e a inocuidade dos produtos lácteos.The veterinary literature registers sporadic cases of infections caused by environmental microorganisms in which the main agents involved are yeasts, yeasts-like and filamentous fungi. In relation to dairy animals, the yeasts are more frequently incriminated as the aetiological agents of mycotic mastitis. Most cases occur under the form of localized outbreaks and / or after treatment with antimicrobial agents. The main genera involved in mastitis are Candida and Cryptococcus, besides others such as Geotrichum, Pichia and Trichosporon. The purpose of this review is to point out the main aspects of mastitis caused by yeasts and yeast-like fungi, such as etiology

  15. Síndrome oculoglandular de Parinaud causada por esporotricose Parinaud's oculoglandular syndrome caused by Sporotrichosis

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    Alexandre Sampaio de Abreu Ribeiro

    2010-10-01

    Full Text Available A síndrome oculoglandular de Parinaud é uma doença ocular rara causada por diferentes agentes etiológicos, entre eles bactérias, vírus e fungos. É caracterizada por uma conjuntivite granulomatosa, acompanhada de linfadenopatia pré-auricular adjacente e pode trazer sequelas caso não seja prontamente tratada. Neste artigo é relatado o caso de uma jovem técnica de enfermagem e estudante de medicina veterinária apresentando a síndrome oculoglandular de Parinaud causada pelo fungo Sporothrix schenkii após contaminação com gatos infectados. Sua apresentação clínica e evolução desfavorável até o correto diagnóstico etiológico e instituição do tratamento específico, ressaltam a importância da investigação laboratorial em casos de evolução atípica da doença.Parinaud's oculoglandular syndrome is a rare eye disease caused by different pathogens, including bacteria, viruses and fungi. It is characterized by a granulomatous conjunctivitis with adjacent preauricular lympha-denopathy and can cause sequelae if not promptly treated. We report a case of a young nurse assistant and veterinary student showing Parinaud's oculoglandular syndrome caused by the fungus Sporothrix schenkii after contamination with infected cats. Its clinical presentation and negative outcome until the correct ethiological diagnosis, in addition to specific treatment, emphasize the importance of laboratory investigations in cases of atypical development of the disease.

  16. Uso de compuestos fenólicos para el tratamiento de patologías causadas por Helicobacter pylori

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    Carrascosa, Alfonso V.; Martínez-Rodríguez, Adolfo J.; López-Brea, Manuel; Alarcón, Teresa; Rodrigo, Sandra

    2010-01-01

    La presente invención se refiere a una serie de compuestos fenólicos que pueden ser extraídos de la uva o del vino y que poseen capacidad bacericida contra Helicobacter pylori, por lo que dichos compuestos son útiles para la fabricación de un medicamento para el tratamiento y/o prevención de patologías causadas por esta bacteria o como aditivos en alimentos funcionales o productos alimentarios.

  17. Uso de compuestos fenólicos para el tratamiento de patologías causadas por helicobacter pylori

    OpenAIRE

    Carrascosa, Alfonso V.; Martínez-Rodríguez, Adolfo J.; López-Brea, Manuel; Alarcón, Teresa; Rodrigo, Sandra

    2010-01-01

    [ES] La presente invención se refiere a una serie de compuestos fenólicos que pueden ser extraídos de la uva o del vino y que poseen capacidad bactericida contra Helicobacter pylori, por lo que dichos compuestos son útiles para la fabricación de un medicamento para el tratamiento y/o prevención de patologías causadas por esta bacteria o como aditivos en alimentos funcionales o productos alimentarios.

  18. Primer caso de onicomicosis causada por Cylindrocarpon lichenicola, en un paciente inmunosuprimido en Costa Rica

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    Mauricio Lizano-Calvo

    2013-12-01

    Full Text Available Antecedentes: la onicomicosis es una infección fúngica de las uñas, que puede ser causada por dermatofitos, levaduras y hongos filamentosos no dermatofitos. El objetivo del presente trabajo fue identificar -morfológica y molecularmente- un aislamiento realizado a partir de la uña del dedo medio de la mano derecha de un paciente inmunosuprimido con trasplante renal. Métodos: a la muestra se le realizó un examen microscópico directo en KOH 40%, y las características de la colonia fueron evaluadas en medio de cultivo Agar Sabouraud Glucosado y Agar Mycosel. El hongo aislado se identificó molecularmente mediante la amplificación, por reacción en cadena de la polimerasa (PCR y secuenciación de la región espaciadora transcrita (ITS de los genes ribosomales y de una porción del gen del factor de elongación 1alfa (EF-1α. La secuencia de nucleótidos de cada región fue comparada con las secuencias publicadas en la base de datos del Banco de Genes del Centro Nacional para la Información Biotecnológica (NCBI. Resultados: las características macro y microscópicas de la colonia concordaron con las descritas para Cylindrocarpon lichenicola (sinónimo Fusarium lichenicola. La secuencia de nucleótidos, tanto de la región ITS (544 pb como de la porción del gen EF-1α (710 pb, presentó un 100% de similitud con secuencias de F. lichenicola. Conclusión: se identificó por primera vez en Costa Rica C. lichenicola asociado con onicomicosis en un paciente inmunosuprimido. Este tipo de pacientes deben ser instruidos sobre las posibles fuentes de agentes infecciosos.

  19. PUDRICIÓN BASAL CAUSADA POR Phytophthora capsici EN PLANTAS DE CHILE TRATADAS CON VERMICOMPOST

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    Lidieth Uribe-Lor\\u00EDo

    2014-01-01

    Full Text Available Pudrición basal causada por Phytophthora capcisi en plantas de chile tratadas con vermicompost. El objetivo de este trabajo fue evaluar a nivel de invernadero el efecto de vermicompost sobre la incidencia y severidad de Phytophthora capcisi en plantas de chile (Capsicum annuum. Esta investigación se realizó entre febrero y marzo del 2012 en el Centro de Investigaciones Agronómicas, Sabanilla, San José, Costa Rica. Se utilizó un diseño completamente al azar con cuatro repeticiones y ocho plantas por unidad experimental en un arreglo factorial con los factores de dosis (0, 25% y 50% v/v vermicompost:suelo e inoculación (0 y 500 zoosporas por gramo de suelo. Se trasplantaron plántulas de 35 días, que fueron inoculadas dos semanas después del trasplante. La aplicación de vermicompost provocó un aumento significativo del peso fresco foliar y del peso seco foliar y radical; a mayor dosis, mayor fue el incremento. Las plantas de todos los tratamientos que incluyeron inoculación con P. capsici presentaron síntomas de la enfermedad en la raíz. Los valores de incidencia y severidad fueron mayores para el tratamiento con 50% de abono. En este tratamiento se presentaron síntomas de marchitez. La ausencia de diferencias en las variables de peso fresco y seco entre los tratamientos inoculados y sin inocular, a los que se adicionó abono al 25%, sugiere que esta dosis podría compensar el daño causado por el patógeno. Se observó una menor concentración de nutrimentos en los tejidos de las plantas inoculadas lo que indica que el daño causado a la raíz pudo haber afectado la adquisición de nutrimentos.

  20. Fístula broncopleural cutânea causada por Eikenella corrodens Bronchopleural cutaneous fistula due to Eikenella corrodens

    OpenAIRE

    Kin-Sun Wong; Yhu-Chering Huang

    2005-01-01

    OBJETIVO: Fazer uma revisão sobre fistula cutânea broncopleural causada por Eikenella corrodens e relatar e discutir o caso de uma paciente. DESCRIÇÃO: Paciente do sexo feminino, 16 anos de idade, foi encaminhada ao nosso hospital em estado febril com histórico de escarro com estrias de sangue há 2 meses; apresentava seqüelas neurológicas de encefalite herpética e estava confinada ao leito desde os cinco anos de idade. A mãe relatou ter detectado, dias antes, uma massa mole paraespinhal longi...

  1. Mancha areolada causada por Thanatephorus cucumeris em mogno africano Target leaf spot caused by Thanatephorus cucumeris on African mahogany

    OpenAIRE

    LUADIR GAPAROTTO; ROGÉRIO E. HANADA; FERNANDO C. ALBUQUERQUE; MARIA DE LOURDES R. DUARTE

    2001-01-01

    O mogno africano (Khaya ivorensis) foi introduzido no Brasil para substituir o mogno brasileiro (Swietenia macrophylla) devido a sua alta resistência ao microlepidóptero Hypsiphyla grandella, a principal praga do mogno brasileiro. Em 1999, observou-se alta incidência da mancha areolada, causada por Thanatephorus cucumeris em viveiros e áreas de plantios definitivos de mogno africano nos Estados do Amazonas e Pará, causando lesões em folhas maduras e 100% de queda das folhas jovens. Nas folhas...

  2. Nuevas aportaciones al diagnóstico de las enfermedades causadas por las micobacterias

    OpenAIRE

    Manterola Martija, Joxe Mari

    2004-01-01

    Las micobacterias se pueden detectar por examen microscópico, por demostración de su ADN o rRNA específico tras amplificación del ácido nucleico correspondiente o por cultivo. Para aislarlas por cultivo, hace falta tratar las muestras con flora comensal (esputos, orinas, etc.) con diferentes sustancias, proceso llamado descontaminación.El objetivo de la Tesis ha consistido en evaluar: a) un nuevo método de descontaminación de muestras clínicas con C18-carboxypropilbetaína (CB-18) y compararl...

  3. Salmonella causada por reptiles y anfibios en guarderías (Salmonella Infections Caused by Reptiles and Amphibians in Childcare Centers)

    Centers for Disease Control (CDC) Podcasts

    2013-02-07

    Traducción al español de la entrevista con el Dr. Neil Vora, funcionario del EIS de los CDC, sobre su artículo acerca de las infecciones por Salmonella causadas por reptiles y anfibios en guarderías.  Created: 2/7/2013 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 2/26/2013.

  4. SISTEMAS DE SIEMBRA EN TRIGO: ENCARANDO LA ESCASEZ DE AGUA CAUSADA POR EL CALENTAMIENTO GLOBAL

    OpenAIRE

    Héctor Eduardo Villaseñor Mir; Agustín Limón Ortega; Eduardo Espitia Rangel; Luis Antonio Mariscal Amaro

    2011-01-01

    En 80% de las regiones productoras de trigo de temporal hay deficiencias de humedad, por lo que la disponibilidad de agua y la eficiencia en su uso son los factores más importantes, para mejorar la rentabilidad del cultivo, y más aún, cuando se prevé que gran parte de México, en los próximos años, enfrentará temporales más escasos por el calentamiento global. La siembra de trigo en camas, con y sin microcuencas, ha sido ideada para una captación efectiva del agua de lluvia, que permite mayor ...

  5. Surdez súbita causada por hemorragia intralabiríntica Sudden hearing loss caused by labyrinthine hemorrhage

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    Raquel Salomone

    2008-10-01

    Full Text Available A surdez súbita sensorioneural é uma perda auditiva súbita ou rapidamente progressiva. Na maioria dos casos a etiologia não é descoberta. Uma das causas possíveis de surdez súbita é a hemorragia intralabiríntica que, antes do surgimento da ressonância magnética, não era corretamente diagnosticada. O objetivo deste trabalho é relatar um caso de surdez súbita causada por hemorragia intralabiríntica e realizar uma revisão da literatura sobre este assunto.Sudden sensorineural hearing loss is relatively frequent. In most cases, the etiology is not discovered. One of the possible causes for sudden deafness is inner labyrinth bleeding, which was difficult to diagnose before the advent of magnetic resonance imaging. The purpose of this paper is to report a case of sudden hearing loss caused by a labyrinthine hemorrhage, and to present a review of the literature on this topic.

  6. Clasificación de malformaciones craneales causadas por craneosinostosis primaria utilizando kernels no lineales

    OpenAIRE

    Salvador Ruiz-Correa; Yerania Campos-Silvestre

    2010-01-01

    La craneosinostosis primaria (CP) es una condición patológica asociada a la fusión prematura de una sutura del calvario. La fusión temprana produce malformaciones craneales severas que se asocian frecuentemente con discapacidades cognitivas, del lenguaje, el aprendizaje y el comportamiento social en general. Por estas razones, la CP representa un área de investigación importante que requiere de métodos efectivos para caracterizar la morfología del complejo craneofacial. En este trabajo, se es...

  7. ANTRACNOSE DO CAQUIZEIRO CAUSADA POR Colletotrichum horii: INCIDÊNCIA EM RAMOS, FOLHAS, FLORES E FRUTOS EM CAMPO

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    RENATO REZENDE YOUNG BLOOD

    2015-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a incidência em várias partes da planta e o desenvolvimento da antracnose do caquizeiro causada por Colletotrichum horiinas cultivares Fuyu e Kakimel produzidas sob os sistemas orgânico e convencional, respectivamente, nas safras de 2010/2011 e 2011/2012, em Campina Grande do Sul, Paraná, Brasil. Em cada pomar, 10 plantas foram selecionadas aleatoriamente, e 10 ramos do ano por planta, distribuídos ao redor dacopa, foram marcados para as avaliações de incidência de antracnose em ramos e folhas, a cada 15 dias. A quantificação de frutos caídos com ou sem sintomas da doença foi a cada 15 dias, durante o período de crescimento de frutos até a colheita, relacionada com a produção total. C. horii em flores foi avaliada em campo e também após incubação no laboratório. Infecção latente foi observada em frutos verdes coletados aos 90; 120 e 150 dias após a floração. Em ambas as safras, observou-se a queda de frutos com sintomas de antracnose entre os meses de janeiro e abril. C. horii é capaz de infectar flores, permanecer latente e provocar sintomas de antracnose em frutos imaturos. Frutos colhidos sadios apresentaram sintomas em pós-colheita após a incubação.

  8. Pancreatite intersticial crônica em bovino causada por Eurytrema coelomaticum

    OpenAIRE

    Pedro Souza Quevedo; Mariana Mendes; Felipe Geraldo Pappen; Mauro Pereira Soares; Gertrud Muller; Nara Amélia da Rosa Farias

    2013-01-01

    Descrevem-se os achados clínicos e patológicos atribuídos à infecção maciça por trematódeos pancreáticos do gênero Eurytrema (Loos, 1907) em uma novilha da raça Jersey, procedente do município de Seara, região oeste do estado de Santa Catarina, Brasil. O animal de dois anos de idade apresentava desenvolvimento insuficiente, emagrecimento crônico, caquexia, decúbito prolongado e anemia, sendo submetido à eutanásia in extremis. Na necropsia observou-se aumento de volume do pâncreas, que estava ...

  9. Comportamiento de la diarrea causada por virus y bacterias en regiones cercanas a la zona ecuatorial.

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    María Fernanda Gutiérrez

    2009-11-01

    Full Text Available INTRODUCCIÓN: La enfermedad diarreica aguda (EDA es un problema de salud a nivel mundial que afecta a la población infantil de distintas regiones. Casi todos los estudios epidemiológicos se han hecho en países con estaciones y poco se informa su comportamiento en países sin estaciones, donde la EDA es endémica con picos epidémicos. OBJETIVOS: Contribuir a conocer la conducta de EDA en Colombia y determinar si su comportamiento es diferente en niños menores de cinco años en dos regiones distintas entre sí en geografía y clima. MATERIALES Y MÉTODOS: Se hizo un estudio descriptivo en dos localidades colombianas. Una en la costa atlántica y otra en el centro del país. La muestra se obtuvo en menores de cinco años que consultaron por diarrea a centros asistenciales de cada región. Los microorganismos bacterianos se identificaron mediante pruebas bioquímicas y los virus con técnicas inmunoenzimáticas. En el análisis estadístico se siguieron un ensayo bivariado y pruebas Z de normalidad para verificar si el clima modifica el comportamiento de EDA y si se presenta de manera distinta en las dos regiones. RESULTADOS: En ambas zonas (Cartagena, Bolívar y Facatativa, Cundinamarca predominó la diarrea viral, frente a la EDA bacteriana. También en ambas el rotavirus fue prevalente. Fue mucho más baja la presencia de astrovirus y adenovirus. No hubo datos con significación estadística para demostrar que las condiciones ambientales y las propias de los niños, alteran el comportamiento de la EDA, pero sí se observó que la EDA por rotavirus se comporta de manera diferente al analizar en forma comparativa las dos regiones del estudio.

  10. Pancreatite intersticial crônica em bovino causada por Eurytrema coelomaticum

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    Pedro de Souza Quevedo

    2013-08-01

    Full Text Available Descrevem-se os achados clínicos e patológicos atribuídos à infecção maciça por trematódeos pancreáticos do gênero Eurytrema (Loos, 1907 em uma novilha da raça Jersey, procedente do município de Seara, região oeste do estado de Santa Catarina, Brasil. O animal de dois anos de idade apresentava desenvolvimento insuficiente, emagrecimento crônico, caquexia, decúbito prolongado e anemia, sendo submetido à eutanásia in extremis. Na necropsia observou-se aumento de volume do pâncreas, que estava esbranquiçado, crepitante, com a superfície de corte irregular, onde foram visualizados inúmeros trematódeos emergindo ativamente do órgão. Histologicamente havia pancreatite intersticial crônica. O parasito foi identificado como Eurytrema coelomaticum (Giard & Billet, 1882. Trata-se do primeiro registro de euritrematose clínica em bovino no estado de Santa Catarina.

  11. Oftalmomiíase externa causada por Dermatobia hominis Dermatobia hominis provoking external ophthalmomyiasis

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    Abelardo de Souza Couto Junior

    2010-10-01

    Full Text Available Relato de caso de oftalmomiíase externa em uma paciente de 82 anos, diabética e hipertensa, residente na zona rural, que inicialmente apresentava um quadro sugestivo de celulite pré-septal e hordéolo, com dor, edema, eritema e nodulação localizada na pálpebra superior e bulbo ocular normal. Foi então tratada com antibiótico e corticóide local, que levou a redução do processo inflamatório, possibilitando a realização de adequado exame físico, com a eversão da pálpebra superior, sendo estabelecido o diagnóstico de oftalmomiíase externa. Após tentativa frustrada de retirada ambulatorial da larva, foi indicado o procedimento cirúrgico por uma incisão vertical no tarso, a qual possibilitou a remoção de larva de 6mm de comprimento, com características da espécie Dermatobia hominis. Diante do exposto, é importante destacar, os vários diagnósticos diferenciais de oftalmomiíase, a particularidade de cada agente, as formas possíveis de tratamento, os fatores de risco e aspectos profiláticos.A 82 years old women reported an external opthalmomyiasis. She was a diabetic and with high blood pressure carrier, living in rural area. Initially a she had pain with swelling, erythema and nodulation located on the upper eyelid and normal eye and a pre-septal cellulitis was suspected and antibiotic and local steroid were introduced, which led to a reduction of inflammatory process and to perform an appropriate physical examination with the eversion of the upper eyelid, which established the diagnosis of an external opthalmomyiasis. After the unsuccessful attempt to outpatient withdrawal of the worm, was appointed the surgical procedure by a vertical incision in the tarsus, which allowed the removal of a larva 6 mm long, with characteristics of the species Dermatobia hominis. Considering the foregoing, it is important to emphasize, the various differencial diagnostics of opthalmomyiasis, the particularity of each agent, possible forms

  12. Costo económico de la discapacidad causada por lesiones de tránsito en México durante 2012

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    Patricia G. Sánchez-Vallejo

    2015-04-01

    Full Text Available Para estimar el costo económico de la discapacidad permanente causada por lesiones de tránsito en México durante 2012, desde las perspectivas del Sistema de Salud y de la Sociedad, se realizó un estudio de costos que utiliza metodología bottom-up, considerando costos directos médicos (hospitalización, consultas ambulatorias y de rehabilitación y prótesis, y costos indirectos (pérdida de productividad del lesionado y cuidador con una aproximación de capital humano. La discapacidad causada por lesiones de tránsito tiene un alto costo para el sistema de salud y la sociedad mexicana. Desde la perspectiva del sistema de salud, el costo en pesos mexicanos de la discapacidad permanente fue de US$269.529.480,72, equivalente a US$1.496,33 por persona. Desde la perspectiva de la sociedad, se estimaron US$3.445,45 durante el primer año. En promedio, se estimó un costo total de US$4.941,77 por persona, resultando en un total de US$1.119.761.632,53 en 2012. Los resultados de este estudio evidencian la necesidad de diseñar e implementar políticas más enérgicas y eficientes para el control de las lesiones de tránsito en México

  13. Jejunal perforation caused by abdominal angiostrongyliasis Perfuração jejunal causada por angiostrongilíase abdominal

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    Jaques WAISBERG

    1999-09-01

    Full Text Available The authors describe a case of abdominal angiostrongyliasis in an adult patient presenting acute abdominal pain caused by jejunal perforation. The case was unusual, as this affliction habitually involves the terminal ileum, appendix, cecum or ascending colon. The disease is caused by the nematode Angiostrongylus costaricensis, whose definitive hosts are forest rodents while snails and slugs are its intermediate hosts. Infection in humans is accidental and occurs via the ingestion of snail or slug mucoid secretions found on vegetables, or by direct contact with the mucus. Abdominal angiostrongyliasis is clinically characterized by prolonged fever, anorexia, abdominal pain in the right-lower quadrant, and peripheral blood eosinophilia. Although usually of a benign nature, its course may evolve to more complicated forms such as intestinal obstruction or perforation likely to require a surgical approach. Currently, no efficient medication for the treatment of abdominal angiostrongyliasis is known to be available. In this study, the authors provide a review on the subject, considering its etiopathogeny, clinical picture, diagnosis and treatment.Os autores descrevem caso de angiostrongilíase abdominal em doente adulto que se manifestou como abdômen agudo devido à perfuração de alça jejunal, evento raro, uma vez que esta afecção geralmente envolve o íleo terminal, apêndice, ceco ou cólon ascendente. A doença é causada pelo nematódeo Angiostrongylus costaricensis cujos hospedeiros definitivos são roedores silvestres e os hospedeiros intermediários são caracóis e caramujos. A infecção em humanos é acidental e ocorre pela ingestão de secreção mucóide destes invertebrados presentes em vegetais ou por contato direto com o muco. A angiostrongilíase abdominal é clinicamente caracterizada pela presença de febre prolongada, anorexia, dor no quadrante inferior direito do abdômen e eosinofilia periférica. Embora a doença seja de

  14. Fístula broncopleural cutânea causada por Eikenella corrodens Bronchopleural cutaneous fistula due to Eikenella corrodens

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    Kin-Sun Wong

    2005-06-01

    Full Text Available OBJETIVO: Fazer uma revisão sobre fistula cutânea broncopleural causada por Eikenella corrodens e relatar e discutir o caso de uma paciente. DESCRIÇÃO: Paciente do sexo feminino, 16 anos de idade, foi encaminhada ao nosso hospital em estado febril com histórico de escarro com estrias de sangue há 2 meses; apresentava seqüelas neurológicas de encefalite herpética e estava confinada ao leito desde os cinco anos de idade. A mãe relatou ter detectado, dias antes, uma massa mole paraespinhal longitudinal. A paciente recebia alimentação via oral apesar da ocorrência de freqüentes engasgamentos nos últimos anos. À apalpação, a massa podia ser pressionada até encontrar menor resistência do espaço subcutâneo, estendendo-se longitudinalmente até a região torácica inferior. A tomografia torácica revelou pneumonia necrotizante do lobo direito inferior e uma fistula bronco pleural formando áreas de cavitação subcutânea. A paciente foi submetida a tratamento prolongado com antibióticos com decrescentes coletas de ar por 8 semanas. COMENTÁRIOS: A Eikenella corrodens tem sido indicada como potencial patógeno causador de infecções pleuropulmonares. Não há relatos de fístula pleurocutânea e formação de abscessos como complicadores de empiema e pneumonia necrotizante causados por infecção de E. corrodens. A presença de lesão torácica proeminente que aumenta e diminui com a respiração pode ser uma indicação de fistula pleurocutânea. O tratamento do empiema de Eikenella com antibióticos sem decorticação cirúrgica exige um tratamento prolongado com antibioticoterapia.OBJECTIVE: The aim of this paper is to review the subject and to report on and discuss a case of bronchopleural cutaneous fistula due to Eikenella corrodens. DESCRIPTION: A 16-year-old girl was brought to our hospital with fever and blood-tinged sputum 2 weeks prior to her admission. She suffered from neurologic sequelae of herpetic encephalitis and

  15. Feo-hifomicose causada por Veronaea bothryosa: relato de dois casos Subcutaneous Phaeohyphomycosis caused by Veronaea bothryosa: report of 2 cases

    OpenAIRE

    Roberto Rheingantz da Cunha Filho; Joel Schwartz; Mariane Rehn; Gerson Vettorato; Maria Aparecida de Resende

    2005-01-01

    Feo-hifomicose causada por Veronaea bothryosa é muito rara. Os autores relatam dois casos, que acreditam ser o primeiro e o segundo no continente americano, mas quarto e quinto da literatura mundial, até o momento. Trata-se de dois pacientes, um transplantado renal e outro imunossuprimido não transplantado. Ambos apresentavam lesões no dorso do pé após trauma. O primeiro respondeu moderadamente ao tratamento com itraconazol, e o segundo respondeu muito bem à excisão cirúrgica.Phaeohyphomycosi...

  16. An unusual gangrenous goat mastitis caused by Staphylococcus aureus, Clostridium perfringens and Escherichia coli co-infection Mastite gangrenosa caprina atípica causada por co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli

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    M.G. Ribeiro

    2007-06-01

    Full Text Available Relata-se, pela primeira vez no Brasil, a ocorrência de mastite gangrenosa caprina atípica causada pela co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli em uma cabra da raça Boer, na segunda semana de lactação. Descrevem-se os achados clínicos, os procedimentos de diagnóstico microbiológico e a conduta terapêutica.

  17. An unusual gangrenous goat mastitis caused by Staphylococcus aureus, Clostridium perfringens and Escherichia coli co-infection Mastite gangrenosa caprina atípica causada por co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli

    OpenAIRE

    M.G. Ribeiro; G.H.B. Lara; S.D. Bicudo; A.V.G. Souza; T. Salerno; A.K. Siqueira; J.S. Geraldo

    2007-01-01

    Relata-se, pela primeira vez no Brasil, a ocorrência de mastite gangrenosa caprina atípica causada pela co-infecção por Staphylococcus aureus, Clostridium perfringens e Escherichia coli em uma cabra da raça Boer, na segunda semana de lactação. Descrevem-se os achados clínicos, os procedimentos de diagnóstico microbiológico e a conduta terapêutica.

  18. Fatores de risco associados à mastite bovina causada por Prototheca zopfii Risk factors associated with bovine mastitis caused by Prototheca zopfii

    OpenAIRE

    Aline Artioli Machado Yamamura; Ernst Eckhardt Müller; Roberta Lemos Freire; Júlio César de Freitas; Lucienne Garcia Pretto-Giordano; Roberta dos Santos Toledo; Márcio Garcia Ribeiro

    2008-01-01

    Este trabalho teve como objetivo o estudo de fatores de risco associados à mastite bovina causada por Prototheca zopfii. Foram analisadas 13 propriedades leiteiras dos Estados do Paraná e de São Paulo, segundo os seguintes critérios de seleção: confirmação prévia de casos de mastite por Prototheca spp., triagem pela pesquisa de Prototheca spp. em tanques de expansão e latões e rebanhos com contagem de células somáticas acima de 5x105cel mL-1. As amostras coletadas consistiram de: leite, água,...

  19. Reação de cultivares de batata à podridão mole causada por Pectobacterium carotovorum subsp. atrosepticum, por P. carotovorum subsp. carotovorum e por P. chrysanthemi Reaction of potato cultivars to soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, P. carotovorum subsp. carotovorum and P. chrysanthemi

    OpenAIRE

    Adilce I. H. Benelli; Norimar D. Denardin; CARLOS A. FORCELINI; Valmir Duarte

    2004-01-01

    A podridão mole em tubérculos de batata (Solanum tuberosum), causada por Pectobacterium carotovorum subsp. atrosepticum, por Pectobacterium carotovorum subsp. carotovorum e por P. chrysanthemi, é uma preocupante doença que causa danos expressivos à cultura em todo o mundo. Como inexiste tratamento eficiente para a podridão mole, o desenvolvimento de cultivares resistentes é considerado o método mais eficaz para a redução de perdas causadas pela doença. Nesse sentido, quatro cultivares de bata...

  20. Podridão de raízes causada por Pythium aphanidermatum, em cultivares de alface produzidas em sistema hidropônico

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    Zayame Vegette Pinto

    2011-12-01

    Full Text Available A podridão de raízes, causada por Pythium aphanidermatum e outras espécies de Pythium, é a principal doença da alface cultivada em sistemas hidropônicos no Brasil. O presente trabalho teve como objetivo avaliar quatro cultivares comerciais de alface em relação à sensibilidade a podridão de raízes, causada por P. aphanidermatum. Os estudos foram realizados em placas de Petri contendo ágar-água com plântulas de alface das cultivares crespa (Vera e Verônica e lisa (Regina e Elisa, infestadas ou não com o patógeno. Com as mesmas cultivares foram realizados quatro experimentos em sistemas hidropônicos (Nutrient Film Technique, sendo dois em estufa coberta com plástico e sombrite e dois em estufa coberta apenas com plástico. As plântulas, infectadas ou não com P. aphanidermatum, foram transplantadas para os sistemas infestados ou não. Foi avaliada a severidade da doença e o desenvolvimento das plantas. Todas as cultivares foram suscetíveis à podridão de raízes nos experimentos realizados in vitro e in vivo. A presença do sombrite não reduziu a podridão de raízes em cultivares de alface produzidas no sistema hidropônico. A cultivar Regina apresentou maior massa de matéria seca da parte aérea e das raízes, na presença ou ausência do patógeno, sendo a mais indicada para o cultivo hidropônico na época mais quente do ano.

  1. Primeira ocorrência de ferrugem em capim-limão causada por Puccinia cymbopogonis no Brasil First report of the lemongrass rust fungi caused by Puccinia cymbopogonis in Brazil

    OpenAIRE

    João Batista Vida; Aníbal Alves de Carvalho Júnior; Jaqueline Rosemeire Verzignassi

    2006-01-01

    È registrada a primeira ocorrência de ferrugem em capim-limão (Cymbopogon citratus (DC.) Stapf), causada por Puccinia cymbopogonis Mass., no Brasil, Estado do Paraná.It is reported the first occurrence of the lemongrass (Cymbopogon citratus (DC.) Stapf) rust fungi caused by Puccinia cymbopogonis Mass. in Brazil, State of Paraná.

  2. Primeira ocorrência de ferrugem em capim-limão causada por Puccinia cymbopogonis no Brasil First report of the lemongrass rust fungi caused by Puccinia cymbopogonis in Brazil

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    João Batista Vida

    2006-03-01

    Full Text Available È registrada a primeira ocorrência de ferrugem em capim-limão (Cymbopogon citratus (DC. Stapf, causada por Puccinia cymbopogonis Mass., no Brasil, Estado do Paraná.It is reported the first occurrence of the lemongrass (Cymbopogon citratus (DC. Stapf rust fungi caused by Puccinia cymbopogonis Mass. in Brazil, State of Paraná.

  3. Candida esophagitis: species distribution and risk factors for infection Esofagite por Candida: distribuição da espécie e fatores de risco para a infecção

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    Dimas Alexandre Kliemann

    2008-10-01

    Full Text Available Although Candida albicans is the main cause of fungal esophagitis, other species such as C. tropicalis, C. krusei and C. stellatoidea have also been implicated. Several studies have identified risk factors for C. albicans esophagitis. However, data for non-C. albicans species is still sparse. The aim of this study was to determine the etiology of Candida esophagitis in our medical centre over an 18-month period. Additionally, we aimed to investigate predisposing conditions for esophageal candidosis caused by different Candida species. A total of 21,248 upper gastroscopies were performed in Santa Casa Complexo Hospitalar between January 2005 and July 2006. The prevalence of Candida esophagitis was 0.74% (n = 158. C. albicans caused the vast majority of infections (96.2%, followed by C. tropicalis (2.5%, C. lusitaniae (0.6% and C. glabrata (0.6%. There were 81 women (51.3% and 77 men (48.7%. No case of mixed infection occurred. Concomitant oral candidosis was documented for 10.8% (n = 17. Most of cases (55.1% involved outpatients. Around one fifth of patients in our cohort had no identifiable risk factors for esophageal candidosis (20.8%. Since nearly all infections were caused by C. albicans we were not able to determine risk factors for esophagitis caused by other Candida species.Embora Candida albicans seja a principal causa de esofagite fúngica, outras espécies como C. tropicalis, C. krusei e C. stellatoidea também têm sido implicadas. O objetivo desse estudo foi descrever espécies causadoras de esofagite fúngica em nosso centro durante um período de 18 meses, além de comparar condições predisponentes para candidose esofágica causadas por diferentes espécies de Candida. De janeiro de 2005 a julho de 2006, 21.248 endoscopias digestivas altas foram realizadas no Complexo Hospitalar Santa Casa (Porto Alegre, Brasil. A prevalência de esofagite por Candida foi de 0,74% (n = 158. C. albicans foi a causadora da maioria das infec

  4. Conidiobolomicose causada por Conidiobolus lamprauges em ovinos no Estado de Santa Catarina Conidiobolomycosis caused by Conidiobolus lamprauges in sheep in the state of Santa Catarina, Brazil

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    Fernando Henrique Furlan

    2010-07-01

    Full Text Available Descreve-se um surto de conidiobolomicose em ovinos no Estado de Santa Catarina. O surto ocorreu entre os meses de dezembro e março de 2006, no município de Santo Amaro da Imperatriz, região litorânea do Estado. A propriedade possuía 75 ovinos da raça Santa Inês e seis desses animais adoeceram. Clinicamente os animais doentes apresentavam dificuldade respiratória, corrimento nasal seroso a mucossanguinolento e, por vezes exolftalmia. Na necropsia verificou-se uma massa amarelada na região etmoidal e adjacências que, às vezes, atingia os linfonodos regionais, cérebro, globo ocular e pleura. Microscopicamente a massa caracterizava-se por infiltrado inflamatório granulomatoso com áreas necróticas associadas a hifas largas pouco ramificadas. Através de exame molecular detectou-se DNA de Conidiobolus lamprauges. Os aspectos clínicos, epidemiológicos, macroscópicos, microscópicos e moleculares caracterizam a conidiobolomicose causada por Conidiobolus lamprauges em ovinos.An outbreak of conidiobolomycosis affecting sheep in the State of Santa Catarina, Southern Brazil is reported. The disease occurred in six Santa Inês breed sheep from a flock of 75 during the rainy season. Common clinical signs were noisy respiration and dyspnea, serous to mucosanguineous nasal discharge and exophthalmus. At necropsy there was a dense yellow mass in the nasopharyngeal area affecting the ethmoidal region, turbinate bones and occasionally limph nodes, central nervous system and pleura. Histopathologycally there was multifocal granulomas whith an eosinophilic necrotic reaction containing ribbon type hyphae similar to zygomycetous fungi. At molecular examination Conidiobolus lamprauges DNA was detected. The clinical, epidemiological, macroscopical, microscopical and molecular aspects characterize conidiobolomycosis caused by Conidiobolus lamprauges in sheep.

  5. Patrones electrocardiográficos en pacientes con hipertrofia ventricular causada por vasculopatía hipertensiva

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    Miguel Moncada

    2001-04-01

    Full Text Available La prueba de oro para el diagnóstico de hipertrofia ventricular
    (HV es el ecocardiograma pero la electrocardiografía ha propuesto
    criterios diagnósticos tanto para HVI como para HVD, aunque la sensibilidad y especificidad no han sido óptimas, dejando siempre a este examen paraclínico por debajo del ecocardiograma en lo que a diagnóstico de HV se refiere (2.

  6. Feo-hifomicose causada por Veronaea bothryosa: relato de dois casos Subcutaneous Phaeohyphomycosis caused by Veronaea bothryosa: report of 2 cases

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    Roberto Rheingantz da Cunha Filho

    2005-02-01

    Full Text Available Feo-hifomicose causada por Veronaea bothryosa é muito rara. Os autores relatam dois casos, que acreditam ser o primeiro e o segundo no continente americano, mas quarto e quinto da literatura mundial, até o momento. Trata-se de dois pacientes, um transplantado renal e outro imunossuprimido não transplantado. Ambos apresentavam lesões no dorso do pé após trauma. O primeiro respondeu moderadamente ao tratamento com itraconazol, e o segundo respondeu muito bem à excisão cirúrgica.Phaeohyphomycosis caused by Veronaea bothryosa is very rare. We report two cases. To our knowledge, these are the first and second cases to be reported on the American continent, and fourth and fifth cases in the world literature. We report one case in a kidney transplant recipient, and another case in an immunosuppressed non-transplant patient. Both patients presented with a lesion on the dorsal aspect of the foot, following trauma. One patient responded moderately well to treatment with itraconazole. The other responded well to a surgical excision.

  7. El estado de las lesiones causadas por el tránsito en México: evidencias para fortalecer la estrategia mexicana de seguridad vial

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    Ricardo Pérez-Núñez

    2014-05-01

    Full Text Available Mediante la revisión de información secundaria oficial, publicaciones científicas y el análisis de la ENSANut-2012, este trabajo busca proveer una visión integral de la magnitud actual de las lesiones causadas por el tránsito (LCT en México y analizar críticamente la respuesta social organizada. Las altas tasas de choques, lesiones, muertes y discapacidad atribuidas a este problema de salud pública, representan un elevado costo para la sociedad mexicana, en especial para los hogares. Se discute cómo la respuesta mexicana se ha enfocado a los automovilistas, dejando fuera a usuarios vulnerables de la vía pública y se ha privilegiado la implementación de estrategias poco efectivas. A pesar de que se dispone de legislación para los principales factores de riesgo, se ha descuidado su correcta aplicación. La estrategia mexicana podría fortalecerse implementando estrategias de movilidad seguras, equitativas, saludables y sostenibles para los diferentes actores de la vía pública. A pesar de los avances que se han dado en términos de prevención de LCT, existen aún retos y oportunidades para el país.

  8. Colonización oral por Candida en pacientes que asisten a una clinica dental in Braga, Portugal

    OpenAIRE

    Martins, M; Henriques, Mariana; A.P Ribeiro; R.M. Fernandes; Gonçalves, Vânia; Seabra, A.; Azeredo, Joana; Oliveira, Rosário

    2010-01-01

    Antecedentes: La capacidad que poseen las diferentes especies de Candida de colonizar las superficies, puede ser considerada como un factor de riesgo para la infección oral. Objetivos: El objetivo de este estudio fue establecer la colonización oral por Candida en pacientes que asisten a una clinica dental en Braga, Portugal. Métodos: Un total de 97 pacientes fueron estudiados. Se colectaron muestras bucales con hisopo y fueron cultivadas directamente en CHROMagar Candida. Las leva...

  9. Desórdenes y enfermedad psiquiátrica causada por el uso de alcohol en Colombia

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    Hernan G. Rincon-Hoyos

    2016-04-01

    Full Text Available Antecedentes: Una comprensión precisa de la co-ocurrencia y la comorbilidad de los trastornos por consumo de alcohol (AUD en Colombia es crucial para la salud pública.Objetivo: Se realizó un análisis secundario, utilizando una base de datos gubernamental de población 2003/2004 para determinar las asociaciones de vida entre los trastornos por consumo de alcohol y otros trastornos mentales y adictivos en personas de edades comprendidas entre 18 a 65 años de Colombia.Métodos: Varios análisis estadísticos se realizaron: pruebas de diferencia en la prevalencia de los trastornos mentales en función de si el individuo tenía un AUD; un análisis estratificado por sexo y análisis de regresión logística teniendo en cuenta las diferencias en las variables del estado de salud, demográficas, socioeconómicas, de comportamiento y de auto-reporte.Resultados: las personas con AUD fueron el 9% de la población, de los cuales 88% eran varones y en promedio tenían 37 años. Estas personas tenían trabajo y eran fumadores activos; y tenían menos probabilidad de estar en casa o ser jubilados. La población con AUD tenía mayores posibilidades de cumplir con los criterios de todos los trastornos, excepto el trastorno depresivo menor, el trastorno de estrés post-traumático, la dependencia a la nicotina, y el trastorno oposicional desafiante.Conclusión: Este estudio demuestra una alta prevalencia de trastornos mentales en la población adulta con trastornos por consumo de alcohol en Colombia. Los resultados destacan la importancia de la comorbilidad como un signo de gravedad de la enfermedad e impacto en la salud pública y apoyan la necesidad de formación de más profesionales y el desarrollo de intervenciones y servicios apropiados.

  10. Pérdidas Humanas y Económicas Causadas por el Vulcanismo en Costa Rica entre 1953 y 2005

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    Irene Aguilar

    2014-12-01

    Full Text Available Se presenta por primera vez una aproximación integrada de los efectos de las erupciones volcánicas en el periodo comprendido entre los años 1953 y el 2005. El detalle de los daños en (puentes, casas, ferrocarril, fábricas, las pérdidas económicas (agricultura, ganadería, suministro de agua potable, interrupción de las actividades, pérdidas de vidas humanas y las inversiones (donaciones, medidas paliativas, se presenta en los correspondientes cuadros, los que se centran en los desastres y efectos económicos, sin embargo los efectos sociológicos y psicológicos no fueron evaluados. Tres emergencias nacionales se han producido, una debido a la erupción del Irazú en 1963-1965, la otra con el Arenal en 1968 y la del volcán Poás en 1990. Pero también varias crisis volcánicas ocurrieron en otros volcanes como el Poás (1953-1955, 1989-1994, Rincón de la Vieja (1967,1991, 1995 y 1998 y de nuevo en Arenal (1975, 1988 y 2000, que produjo las pérdidas económicas debidas a la lluvia acida y/o lahares, otras personas murieron debido a depósitos de flujos piroclásticos. La actividad volcánica es la responsable de al menos 48,7 millones dólares en el último siglo, cantidad que será necesaria escalar en un futuro estudio. En el presente siglo, el daño por la lluvia ácida es de más de $200 mil. Por el momento, se tiene certeza que 103 personas fueron muertas por la actividad volcánica en el siglo XX; no hay informes adicionales de muertes o personas heridas desde 1700. Este documento es la base para un trabajo futuro más centrado en la evaluación socioeconómica, el análisis del riesgo y su proyección hacia el futuro. Si una nueva erupción similar a la del Irazú en 1963-1965 o del Arenal en 1968, sucediera durante el presente siglo, las pérdidas económicas serán significativamente mayores que las anteriores.

  11. Pérdidas Humanas y Económicas Causadas por el Vulcanismo en Costa Rica entre 1953 y 2005

    OpenAIRE

    Irene Aguilar; Guillermo E. Alvarado

    2014-01-01

    Se presenta por primera vez una aproximación integrada de los efectos de las erupciones volcánicas en el periodo comprendido entre los años 1953 y el 2005. El detalle de los daños en (puentes, casas, ferrocarril, fábricas), las pérdidas económicas (agricultura, ganadería, suministro de agua potable), interrupción de las actividades, pérdidas de vidas humanas y las inversiones (donaciones, medidas paliativas), se presenta en los correspondientes cuadros, los que se centran en los desastres y e...

  12. Síndrome respiratória aguda grave causada por influenza A (subtipo H1N1) Severe acute respiratory syndrome caused by the influenza A (H1N1) virus

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    Sandra Aparecida Ribeiro; Graziela Sgreccia Brasileiro; Luciana Novaes Campello Soleiman; Cristiano Cruz Silva; Cláudio Shoki Kavaguti

    2010-01-01

    Frente à pandemia causada por um novo vírus, influenza A (H1N1), descrevemos o caso de um paciente de 56 anos com síndrome respiratória aguda grave causada por influenza A (H1N1) sem fatores de risco importantes. Os resultados dos exames laboratoriais e de imagem (radiografia e TC de tórax) são apresentados aqui. O paciente teve boa evolução e recebeu alta hospitalar em 14 dias.In view of the pandemic caused by a new virus, influenza A (H1N1), we report the case of a 56-year-old patient witho...

  13. La marea roja causada por el dinoflagelado Alexandrium tamarense en la costa Pacífica colombiana (2001

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    Ingrid García-Hansen

    2004-09-01

    Full Text Available El 26 de marzo de 2001 se registró por primera vez en las aguas del Pacífico colombiano, en el área de la Ensenada de Tumaco, la aparición de una marea roja producida por la especie Alexandrium tamarense con valores que superaron las 7.5 x 10(6 céls l-1 , la marea se mantuvo hacia la región oceánica, derivando en sentido sur-norte por efecto de las corrientes, hasta ser vista por ultima vez cerca a la Isla Gorgona, casi tres semanas después. Un año mas tarde, en marzo de 2002, una segunda proliferación, conformada por la misma especie, se reportó cerca de Cabo Corrientes con una concentración de 1.6 x 10(6 céls l -1 . Ambos casos estuvieron relacionados con la presencia de bajas temperaturas en la superficie del mar y no estuvieron asociadas con fenómenos de intoxicación y mortalidadFrom April 26th to May 15th 2001, a large algae bloom was observed off Tumaco Bay on the Pacific coast of Colombia. This was the first harmful algae bloom (HAB reported in the region, and reached Gorgona Island, about 120 km north. Ayear later, starting March 2002, an offshore HAB developed from Cabo Corrientes North to Solano Bay. The typical abundance during the blooms reached 7.5 x 10(6 cells l-1 for the 2001 event and 1.6 x 10(6 cells l-1 for the 2002 event. During both events, low temperature and high salinity were recorded. Typical measurements in the area are 27-27.5°C and 30-31.5 psu. Values observed during the two events were 24-24.6°C and 33-34 psu; 3°C below normal and more than 2.5 psu above average values. These conditions are indicative of local upwelling processes at the time of the events. On both occasions, cells corresponding to the Alexandrium catenella/fundeyense/tamarense complex represented 99-100% of the biomass. It was difficult to differentiate the cells from A. catenella, but the presence of short chains of only 4 cells (single cells represented most of the biomass was suggestive of A. tamarense. Shape, dimensions, and

  14. Fatores de risco associados à mastite bovina causada por Prototheca zopfii Risk factors associated with bovine mastitis caused by Prototheca zopfii

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    Aline Artioli Machado Yamamura

    2008-06-01

    Full Text Available Este trabalho teve como objetivo o estudo de fatores de risco associados à mastite bovina causada por Prototheca zopfii. Foram analisadas 13 propriedades leiteiras dos Estados do Paraná e de São Paulo, segundo os seguintes critérios de seleção: confirmação prévia de casos de mastite por Prototheca spp., triagem pela pesquisa de Prototheca spp. em tanques de expansão e latões e rebanhos com contagem de células somáticas acima de 5x105cel mL-1. As amostras coletadas consistiram de: leite, água, solo, fezes e swab de teteiras. Prototheca spp. foi isolada de amostras de leite dos quartos mamários com mastite clínica ou subclínica em uma propriedade e de amostras de leite e do ambiente em quatro propriedades, nas quais foi isolada em amostras de: água de bebedouro, abastecimento, esgoto, empoçada no piso de estábulo e sala de ordenha, solo de piquete e pasto, teteiras, fezes de bezerros e suínos. Do total de 383 vacas examinadas, Prototheca spp. foi isolada em 20 (5,2% vacas, sendo caracterizada como P. zopfii em 18. Os fatores de risco associados à mastite causada por P. zopfii foram: criação das vacas a pasto, alimentação dos animais com pasto e silagem, realização de ordenha mecânica em estábulo, permanência das vacas após ordenha em piquete sem alimento, criação de suínos próxima às instalações dos bovinos, existência de cães, gatos e roedores, falta de higienização dos tetos com água, pré-imersão dos tetos em aplicador com retorno e sem a troca do anti-séptico, alimentação dos bezerros com leite de vacas com mastite clínica e serem as vacas da raça holandesa.This research had as objective the study of risk factors associated with bovine mastitis caused by Prototheca zopfii. Thirteen dairy herds in Paraná and São Paulo states were analyzed and selected according to the following criteria: previous confirmation of Prototheca spp. mastitis cases, screening of Prototheca spp. in bulk tanks and

  15. Cura espontânea da leishmaniose causada por Leishmania Viannia Braziliensis em lesões cutâneas

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    Jackson Maurício Lopes Costa

    1990-12-01

    Full Text Available Os autores relatam que durante 14 anos de trabalho clínico em campo, realizado nas comunidades de Três Braços e Corte de Pedra, Bahia, acompanharam 1.416 pacientes portadores de Leishmaniose Tegumentar Americana, cuja espécie envolvida na transmissão, é predominantemente a Leishmania Viannia brasilienses. A terapêutica utilizada rotineiramente nos casos é o antimoniato-N-metilglucamina (Glucantime. Contudo, 16 pacientes do sexo masculino recusaram-se a utilizar a medicação e 6 do sexo feminino encontravam-se em período gestacional, portanto não utilizaram o medicamento. Estes pacientes foram acompanhados por um período entre 4 a 12 anos, a partir do diagnóstico. Observou-se que em 9 pacientes (40,9% desta casuística, o tempo de cicatrizaçâo após o aparecimento da lesão, pode ser calculado em 6 meses de evolução. Quando se eleva a observação para 12 meses, temos que 19 pacientes (86,3% cicatrizaram suas lesões neste período. Em 3 casos (13,6% as lesões permaneceram ativas por mais de 12 meses. Conclui-se que os determinantes da cicatrizaçâo natural das lesões produzidas por Leishmania Viannia Braziliensis permanecem desconhecidos, dificultando para nós entendermos e compararmos aos efeitos das drogas utilizadas no tratamento da leishmaniose tegumentar.In field clinics in the comunities of Três Braços and Corte de Pedra, Bahia, we have attended 1.416 patients with tegumentary leishmaniasis in fourteen years, the predomi nant species in transmission is Leishmania Viannia brasiliensis (LVB. Because of the danger of metastasis with this infection treatment was routinely recomended with Glucantime. However sixteen patients refused injection therapy and six women were pregnant when seen and not treated. All patients were followed up in our clinic. All these patients closed their skin ulcers although one subsequently relapsed. Patients were followed up for variable periods (four to twelve years, after the diagnosis. In

  16. Mancha de mirotécio em algodoeiro causada por Myrothecium roridum Myrothecium leaf spot of cotton caused by Myrothecium roridum

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    Maurício Conrado Meyer

    2006-09-01

    Full Text Available A mancha de mirotécio causada por Myrothecium roridum Tode ex Fr. foi observada em lavouras de algodão no sul do Maranhão, causando reduções de produtividade de até 60%. Os sintomas da doença são lesões necróticas, circulares, com estruturas salientes, os esporodóquios, de distribuição irregular. Foram observadas lesões nos pecíolos, brácteas, folhas e maçãs de algodoeiro cv. Deltapine Acala 90, Fibermax 966 e Sure Grow 821. O isolamento do fungo foi realizado em meio de cultura batata-dextrose-ágar (BDA. O teste de patogenicidade foi realizado em maçãs sadias, destacadas de algodoeiro cv. Fibermax 966, no estádio vegetativo R6, previamente desinfestadas. Foram testados 13 isolados de M. roridum, oito provenientes de algodão e cinco de soja. Avaliações das estruturas fúngicas foram realizadas com auxílio de microscópio óptico equipado com um micrômetro ocular. Os isolados causaram infecções em maçãs de algodão e destacou-se como mais agressivo o MA-75, proveniente de algodão, apresentando diâmetro médio de lesão de 1,3cm, aos sete DAI e 2,7cm aos 14 DAI. Todos os isolados formaram esporodóquios dispostos concenticamente em meio BDA. Os conídios são unicelulares, hialinos a oliváceos, abundantemente produzidos em massa verde-oliva a preta. Os conídios de isolados provenientes de algodão mediram, em média, 5,1µm x 1,5µm, e os obtidos de soja, 5,8µm x 1,5µm. Estes resultados relatam a ocorrência da mancha de mirotécio, causada por M. roridum, em lavouras comerciais de algodão no Brasil.Myrothecium leaf spot caused by Myrothecium roridum Tode ex Fr. was observed on cotton fields in Maranhão State, Brazil, causing yield reduction of up to 60%. Disease symptoms are lesions with concentric necrotic rings, with salient structures (sporodochia irregularly distributed. Symptoms were observed on petioles, bracts, leaves and bolls of cotton cultivars Deltapine Acala 90, Fibermax 966 and Sure Grow 821

  17. ALTERAÇÕES MICROMETEOROLÓGICAS CAUSADAS POR TÚNEIS BAIXOS DE TELA PLÁSTICA PRETA CULTIVADOS COM ALFACE

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    Galileo Adeli Buriot

    1994-01-01

    Full Text Available RESUMO Foram determinadas alterações micrometeorológicas causadas por tela plástica preta com 18, 30 e 50% de atenuação da radiação solar incidente, durante os meses de verão, em Santa Maria, RS. O experimento foi conduzido no Campo Experimental do Departamento de Fitotecnia da UFSM, onde foram cultivados quatro canteiros com alface, três deles cobertos com túneis de tela plástica. O estudo foi realizado em 3 períodos: de 30/11/90 a 17/01/91, de 06/02/91 a 25/03/91 e de 10/12/91 a 01/03/92. Mediu-se a radiação solar global, radiação solar na faixa de comprimento de onda do infravermelho e a luminosidade, temperatura diurna do solo, temperatura mínima e diurna do ar e umidade do solo e do ar. Os resultados mostraram que a temperatura do solo e do ar e a perda de água do solo são menores sob os túneis do que no exterior. As diferenças cresceram com o aumento da atenuação e a temperatura mínima do ar e a umidade relativa do ar são semelhantes no interior e exterior dos túneis e entre os túneis com diferentes percentagens de atenuação.

  18. Toxinas killer e produção de enzimas por Candida albicans isoladas da mucosa bucal de pacientes com câncer Killer toxin sensitiviy and production of enzymes by Candida albicans isolated from the oral mucosa of patients with cancer

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    Elida Elias de Oliveira

    1998-12-01

    Full Text Available Infecções oportunistas da cavidade bucal são primariamente causadas por fungos do gênero Candida e freqüentemente ocorrem em pacientes com câncer que estão sobtratamento quimioterápico e antibacteriano. De 44 amostras coletadas da mucosa oral de pacientes com câncer, observou-se o isolamento de 25 leveduras do gênero Candida em cultivo realizado em ágar Sabouraud-dextrose. Foram identificados Candida albicans em 24 (96% isolados e C. krusei em 1 (4%. As características fenotípicas das amostras de Candida albicans mostraram que todos os isolados foram fortemente proteolíticos, capazes de produzir fosfolipases e possuíam os biotipos caracterizados como 811(95,8% e 511 (4,2% em relação a susceptibilidade às toxinas killer.Opportunistic infections of the oral cavity are primarily caused by Candida and frequently occur in patients with cancer who are undergoing chemotherapy and antibiotic treatment. Of the specimens received from the oral mucosa of 44 patients with cancer, 25 (56.8% yielded Candida on culture in Sabouraud agar. Twenty four of these isolates were identified as C. albicans (96% and 1 as C. krusei (4%. The phenotypic characteristics of these isolates showed that all of them were strongly proteolytic, had a high ability to produce phospholipase, and presented the byotypes characterized as 811 (95.8% and 511 (4.2% in terms of susceptibility to killer toxins.

  19. Utilização da vacina Escherichia coli J5 na imunização de vacas leiteiras contra mastites causadas por E. coli

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    Lívio R. Molina

    2013-03-01

    Full Text Available Avaliou-se a utilização da vacina Escherichia coli J5, na imunização de vacas leiteiras, para prevenção e controle da mastite causada por E. coli através da análise da prevalência das infecções intramamárias (IMM no pós-parto, ocorrência e intensidade dos casos clínicos de mastite nos primeiros 100 dias de lactação, influência na contagem de células somáticas (CCS e produção de leite. O grupo experimental foi composto de 187 animais, divididos em 2 grupos, vacas vacinadas e não vacinadas. As imunizações ocorreram 60 dias antes do parto, 30 dias antes do parto e na primeira semana pós-parto. No dia da secagem e sete dias após o parto foram coletadas amostras para diagnóstico microbiológico dos patógenos causadores de mastite. A ocorrência de casos clínicos foi verificada pelo teste da caneca durante as ordenhas sendo registrados os dados relacionados à intensidade. Amostras foram coletadas mensalmente, a partir do décimo dia de lactação, para avaliação da CCS. A produção de leite foi registrada mensalmente nos primeiros 100 dias de lactação. Verificou-se no grupo vacinado, redução na prevalência de E. coli no pós-parto, na ocorrência de casos clínicos por E.coli nos primeiros 100 dias de lactação bem como na intensidade destes casos clínicos. Não foram observadas diferenças estatísticas significativas na CCS, entretanto vacas vacinadas apresentaram maior produção de leite, comparadas às vacas não vacinadas. A vacinação com E. coli J5 foi eficaz em reduzir a prevalência de infecções intramamárias (IMM ao parto, ocorrência e intensidade dos casos clínicos e aumento na produção de leite nos primeiros 100 dias de lactação.

  20. Método FAMACHA para detectar anemia clínica causada por Haemonchus contortus em cordeiros lactentes e ovelhas em lactação

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    Maria Angela M. Fernandes

    2015-06-01

    Full Text Available Resumo: O controle das endoparasitoses gastrintestinais em pequenos ruminantes tem sido tradicionalmente realizado por meio do uso indiscriminado dos anti-helmínticos, porém essa prática tem resultado em grande pressão de seleção de parasitas resistentes. Métodos seletivos (ex: FAMACHA/FMC e contagem de ovos nas fezes/OPG e que apresentem boa sensibilidade para identificar os animais que necessitam receber o tratamento químico são importantes alternativas aos métodos tradicionais. O objetivo desse trabalho foi determinar a sensibilidade e a especificidade do método FMC, usado para detectar anemia causada por Haemonchus contortus em ovinos da raça Suffolk, em duas categorias de alta suscetibilidade; cordeiros lactentes e ovelhas durante a fase de lactação. A conjuntiva ocular de 42 cordeiros lactentes e 35 ovelhas em lactação foi avaliada usando o método FMC, em intervalos de 14 dias, durante cinco meses. O hematócrito (Ht foi utilizado como padrão-ouro para avaliação clínica de anemia dos animais. Para o cálculo de sensibilidade e especificidade, diferentes critérios foram utilizados: animais classificados como 4 e 5 ou 3, 4 e 5 e anêmicos pelo Ht (teste positivo; animais classificados como 1, 2 e 3 ou 1 e 2 e não anêmicos pelo Ht (teste negativo. Três valores de corte para Ht (≤22%, ≤19% ou ≤15% foram utilizados para confirmar a anemia. Entre os gêneros observados nas coproculturas, H. contortus (61,5% e Ostertagiasp (21,3% foram os mais prevalentes. Em ambas as categorias, a inclusão do FMC 3 como teste positivo, elevou a sensibilidade mas reduziu a especificidade do método. Quando o FMC 3 foi considerado anêmico, o percentual de falsos negativos foi próximo a zero, no entanto, houve aumento no número de tratamentos aplicados em animais não anêmicos (falso positivo. Durante as avaliações, a maior parte dos cordeiros (73% e das ovelhas (53% permaneceu nas categorias de FMC 1 e 2. Apenas 5% dos

  1. Incidência da varíola, causada por Asperisporium caricae, em folhas de mamoeiros submetidos ao manejo orgânico, em diferentes ambientes de cultivo

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    Luiz Aurélio Peres Martelleto

    2009-12-01

    Full Text Available Mudas de mamoeiro da cultivar Baixinho de Santa Amália foram transplantadas para covas de 40x60x40 cm, em áreas de três estruturas contíguas: (a estufa sombreada (cobertura de plástico, (b estufa sombreada + sombrite (cobertura adicional de sombrite com 30% de sombreamento sobre o plástico e (c telado (cobertura exclusiva de sombrite 30%. Ao lado de tais estruturas foi implantada uma área de cultivo de mamoeiro em ambiente natural. Os tratos culturais aplicados foram os condizentes às normas técnicas vigentes na agricultura orgânica. As irrigações foram procedidas com mangueira plástica, evitando-se molhar folhas e frutos. Aos 45 dias pós-transplantio e, subseqüentemente, a intervalos mensais, as plantas foram inspecionadas em relação à incidência de lesões foliares causada pelo fungo Asperisporium caricae. Para efeito de análise estatística, após o teste de homogeneidade das variâncias, foram consideradas quatro repetições por ambiente (tratamento, com seis plantas úteis por parcela. O modelo de quantificação da doença indicou efeito altamente significativo dos ambientes protegidos, estufa e estufa sombreada, quanto à incidência de sintomas, em comparação com ambientes de telado e em área natural de cultivo. Durante os 12 meses de avaliações foi constatada alta correlação entre incidência da doença e pluviosidade e umidade relativa do ar. As estruturas cobertas com plástico demonstraram alto potencial de controle de A. caricae, sendo, portanto, recomendáveis no sistema orgânico de produção do mamoeiro.Transplants of Baixinho de Santa Amália papaya cultivar were grown in three neighboring plant growth facilities: a greenhouse (covered with plastic sheet; b shaded greenhouse (with sombrite 30% black screen over the plastic sheet; c screenhouse (covered with sombrite 30% only. Next to such structures a plot was settled for cultivation under natural environment. Cultural practices were conducted

  2. Candida bloodstream infection: data from a teaching hospital in Mato Grosso do Sul, Brazil Infecção na corrente sangüínea por Candida spp. dados de um hospital universitário em Mato Grosso do Sul, Brasil

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    Marilene Rodrigues Chang

    2008-10-01

    Full Text Available The incidence of Candida bloodstream infection has increased over the past years. In the Center-West region of Brazil, data on candidemia are scarce. This paper reports a retrospective analysis of 96 cases of Candida bloodstream infection at a Brazilian tertiary-care teaching hospital in the state of Mato Grosso do Sul, from January 1998 to December 2006. Demographic, clinical and laboratory data were collected from medical records and from the hospital's laboratory database. Patients' ages ranged from three days to 92 years, with 53 (55.2% adults and 43 (44.8% children. Of the latter, 25 (58.1% were newborns. The risk conditions most often found were: long period of hospitalization, utilization of venous central catheter, and previous use of antibiotics. Fifty-eight (60.4% patients died during the hospitalization period and eight (13.7% of them died 30 days after the diagnosis of candidemia. Candida albicans (45.8% was the most prevalent species, followed by C. parapsilosis (34.4%, C. tropicalis (14.6% and C. glabrata (5.2%. This is the first report of Candida bloodstream infection in the state of Mato Grosso do Sul and it highlights the importance of considering the possibility of invasive Candida infection in patients exposed to risk factors, particularly among neonates and the elderly.RESUMO A incidência de infecções na corrente sangüínea causada por Candida spp. tem aumentado nos últimos anos. Na região Centro-Oeste do Brasil, os dados sobre candidemia são escassos. Realizamos uma análise retrospectiva de casos de infecção na corrente sangüínea por Candida em um hospital terciário de ensino de Mato Grosso do Sul. Noventa e seis episódios diagnosticados de janeiro de 1998 a dezembro de 2006 foram incluídos no estudo. Os dados demográficos e clínicos foram obtidos de prontuários; os dados laboratoriais provieram de registros do laboratório hospitalar. Dos pacientes, 43 (44,8% eram crianças e 53 (55,2% adultos, com idades

  3. La necrosis bacteriana de la vid, causada por xylophilus ampelinus. Detección serológica, distribución en Aragón y sensibilidad varietal.

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    CAMBRA ALVAREZ, MIGUEL

    2011-01-01

    La necrosis bacteriana de la vid, causada por la bacteria de cuarentena Xylophilus ampelinus, es una enfermedad de difícil control químico y que ha causado en España importantes pérdidas económicas. Actualmente, es endémica en distintas zonas de Aragón y Galicia y se han identificado focos aislados en La Rioja. Ante la carencia de métodos sensibles y específicos de detección de esta bacteria que se puedan utilizar para el análisis rutinario, se ha puesto a punto un método de ex...

  4. Atividade antifúngica de óleos essenciais sobre espécies de Candida Antifungal activity from essential oils on Candida species

    OpenAIRE

    Igara de Oliveira Lima; Rinalda de Araújo Guerra Oliveira; Edeltrudes de Oliveira Lima; Nilma Maria Porto de Farias; Evandro Leite de Souza

    2006-01-01

    A candidíase apresenta-se como uma infecção fúngica superficial ou profunda causada por leveduras pertencentes ao gênero Candida, sendo considerada a principal infecção micótica em ambiente nosocomial. O objetivo do presente estudo foi determinar a concentração inibitória mínima - CIM do óleo essencial de Cinnamomum zeylanicum Blume, Citrus limon Risso, Eucalyptus citriodora HK, Eugenia uniflora L., Peumus boldus Benth e de Rosmarinus officinialis L. sobre cepas de Candida albicans, C. guilli...

  5. Perdas de rendimento de grãos de soja causadas por interferência de picão-preto e guanxuma Soybean grain yield losses due to interference by beggatticks and arrowleaf sida

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    Mauro Antônio Rizzardi

    2003-08-01

    Full Text Available O grau de interferência mútua entre plantas daninhas e cultivadas pode ser afetado pelas espécies concorrentes e por seu nível populacional. O objetivo deste trabalho foi avaliar a possibilidade de uso do modelo da hipérbole retangular na previsão das perdas de rendimento de grãos de soja causadas por picão-preto (Bidens spp. e guanxuma (Sida rhombifolia L.. Para isso, foram realizados dois experimentos em campo no Município de Eldorado do Sul-RS, nos anos de 1999/00 e 2000/01. Os tratamentos constaram de densidades de plantas de picão-preto ou de guanxuma, as quais foram alocadas de acordo com níveis populacionais de ervas encontrados naturalmente na área experimental. Constatou-se que as perdas de rendimento causadas por incrementos nas densidades de picão-preto e de guanxuma aumentaram de forma não linear. O modelo da hipérbole retangular propicia melhores ajustes quando utiliza perdas do rendimento de grãos do que perdas da matéria seca da soja no florescimento ou do rendimento biológico da soja. por meio da comparação dos parâmetros obtidos para o modelo testado, conclui-se que picão-preto interfere mais negativamente na cultura do que guanxuma.The level of reciprocal interference between weeds and crops is affected by the competing species and plant populations involved. This research was conducted in order to evaluate the possibility of using the retangular hyperbolic model for predicting soybean grain yield losses caused by beggarticks (Bidens spp. and arrowleaf sida (Sida rhombifolia L. infestations. To accomplish this, there were carried out two field experiments in Eldorado do Sul, RS, Brazil, during the 1999/00 and 2000/01 growing seasons. Treatments consisted of beggarticks and arrowleaf densities, which were located according to naturally ocurring population levels in the research area. Was detected that grain yield losses due to increasing densities of these weeds increased non-linearly. The retangular

  6. HLA in Brazilian Ashkenazic Jews with chronic dermatophytosis caused by Trichophyton rubrum Antígenos Leucocitários Humanos (HLA em Judeus Ashkenazitas Brasileiros portadores de dermatofitose crônica causada por Trichophyton rubrum

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    Aya Sadahiro

    2004-06-01

    Full Text Available The frequency of HLA (Human Leucocyte Antigens was analyzed in 25 non-consanguineous Brazilian Ashkenazic Jews, resident in the city of São Paulo, Brazil, suffering from chronic dermatophytosis caused by T. rubrum, and in 25 non-infected individuals belonging to the same ethnic group. Statistically significant values (pA freqüência dos HLA foi analisada em 25 Judeus Ashkenazitas, não consangüíneos, residentes em São Paulo, Brasil, com dermatofitose crônica causada por T. rubrum e em 25 indivíduos sadios, pertencentes ao mesmo grupo étnico dos pacientes. Observou-se valor estatisticamente significante (p<0,05 para HLA-B14 associado a resistência à dermatofitose crônica enquanto HLA-DQB1*06 (p=0,05 possivelmente relacionado a susceptibilidade. Estes achados indicam que o desenvolvimento da dermatofitose crônica pode ser influenciado por genes localizados no cromossomo 6, na região do complexo principal de histocompatibilidade.

  7. OCCURRENCE OF LEAF SPOTS CAUSED BY FUNGI AND A BACTERIUM ON COTTON CULTIVARS OCORRÊNCIA DE MANCHAS FOLIARES CAUSADAS POR FUNGOS E BACTÉRIA EM CULTIVARES DE ALGODOEIRO

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    Miguel Ferreira Soria

    2008-11-01

    Full Text Available

    The cotton leaf spot diseases caused by fungi and bacteria are a growing problem, leading to an increasing use of fungicides. During the 2000/2001 and 2001/2002 growing seasons, field experiments were carried out in a randomized block design, with five replications, aiming to evaluate the behavior of the cotton cultivars Ita-90, CD-401, CD-402, CD-403, CD-404, Fibermax 986, DeltaOpal, and Facual, as related to leaf spot diseases caused by Stemphylium spp., Alternaria spp., Ramularia areola, and the bacterium Xanthomonas axonopodis pv. malvacearum. The cotton cultivars Ita-90 and Facual were the less susceptible to the Stemphylium/Alternaria complex and R. areola. However, Ita-90 was more susceptible to X. axonopodis pv. Malvacearum. The cultivars with the least incidence of X. axonopodis were CD-403, Fibermax 986, and DeltaOpal. The cotton cultivar Facual presented the lowest yield, while the remaining cultivars showed similar yields.

    KEY-WORDS: Xanthomonas axonopodis pv. Malvacearum; Stemphylium spp; Alternaria spp; Ramularia areola.

    As manchas foliares causadas por fungos e bactérias no algodoeiro são um problema cada vez mais grave, levando à utilização, cada vez maior, de fungicidas para controlá-las. Nas safras de 2000/2001 e 2001/2002, foram realizados experimentos m campo, em delineamento de blocos ao acaso, com cinco epetições, objetivando avaliar o comportamento das

  8. Aspectos epidemiológicos de pacientes com lesões ungueais e cutâneas causadas por Scytalidium spp Epidemiological aspects of patients with ungual and cutaneous lesions caused by Scytalidium spp

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    Ana Paula Martins Xavier

    2010-12-01

    Full Text Available FUNDAMENTO: As dermatomicoses causadas por fungos filamentosos não dermatófitos são infecções raras, exceto as onicomicoses, cuja prevalência vem crescendo nos últimos anos. Dentre esses agentes etiológicos destacam-se o Scytalidium dimidiatum e o S. hyalinum, fungos emergentes responsáveis por micoses em unhas e pele. OBJETIVO: Investigar as características epidemiológicas das onicomicoses e micoses de outras localizações causadas pelos fungos do gênero Scytalidium, utilizando-se como parâmetros sexo, idade e localizações das lesões. MÉTODOS: Avaliaram-se 81 amostras com cultura positiva para o gênero em estudo, oriundas de 74 pacientes encaminhados ao Laboratório de Investigação em Dermatologia (ID situado na cidade do Rio de Janeiro (RJ, no período de 1997 a 2006. As amostras foram submetidas a confirmação diagnóstica por exame direto e cultura. RESULTADOS: A prevalência de onicomicoses por Scytalidium spp. foi de 0,87%, entre as idades de 41 e 60 anos (48,64%. Em relação à localização das lesões, os pés foram mais acometidos (91,36%, com predomínio do hálux esquerdo. No exame direto, as estruturas mais encontradas foram hifas hialinas; na cultura, a espécie S. dimidiatum foi a mais frequente. CONCLUSÃO: As onicomicoses por Scytalidium spp. são raras e o S. dimidiatum foi a espécie mais isolada neste laboratório no período em estudo.BACKGROUND: Dermatomycoses caused by non-dermatophyte filamentous fungi are rare infections, except for onychomycosis, whose prevalence has increased over the past few years. Among these etiologic agents, we highlight Scytalidium dimidiatum and S. hyalinum, emergent fungi that cause mycoses that affect the nails and skin. OBJECTIVE: To investigate the characteristics of onychomycosis and other mycoses caused by the fungi Scytalidium spp, using sex, age and site of infection as parameters. METHODS: Eighty-one samples were evaluated showing positive culture for Scytalidium

  9. Restrições causadas pela incontinência urinária à vida da mulher Restricciones ocasionadas por la incontinencia urinaria en la vida de la mujer Urinary incontinence restrictions in women's life

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    Maria Helena Baena de Moraes Lopes

    2006-03-01

    Full Text Available O objetivo deste estudo foi identificar as restrições causadas pela incontinência urinária (IU à vida da mulher, considerando-se o tipo de incontinência, e verificar como manejam este problema. Trata-se de uma análise secundária de dados obtidos em estudo anterior, coletados por meio de entrevista com perguntas abertas e fechadas. A amostra foi composta por 164 mulheres com queixa de IU, internadas em clínicas de ginecologia e urologia de dois hospitais-escola da cidade de Campinas, SP. Apresentavam idade entre 25 e 85 anos e 104 (64% referiram uma ou mais restrições. Citaram alterações nas atividades sexuais (40,9%, sociais (33,5%, domésticas (18,9% e ocupacionais (15,2%. A incontinência urinária mista e a de urgência forem as que mais afetavam a vida das mulheres. A estratégia mais utilizada para minimizar a IU foi o uso de forro e/ou absorvente higiênico. Concluiu-se que a IU tem implicações negativas no cotidiano dessas mulheres.En este estudio se tuvo como objetivo identificar las restricciones causadas por la incontinencia urinaria (IU en la vida de la mujer, considerándose el tipo de incontinencia, y verificar cómo manejan este problema. Se trata de un análisis secundario de datos obtenidos en un estudio anterior, recolectados por medio de entrevista con preguntas abiertas y cerradas. La muestra estuvo compuesta por 164 mujeres con queja de IU, internadas en clínicas de ginecología y urología de dos hospitales docentes de la ciudad de Campinas, SP. Las mujeres tenían entre 25 y 85 años de edad, habiendo relatado 104 (64% de ellas una o más restricciones. Citaron alteraciones en las actividades sexuales (40,9%, sociales (33,5%, domésticas (18,9% y ocupacionales (15,2%. La incontinencia urinaria mixta y la de urgencia fueron las que más afectaban la vida de las mujeres. La estrategia más utilizada para minimizar la IU fue el uso de paños y/o toallas higiénicas. Se concluyó que la IU tiene implicancias

  10. Evaluación de la mutagenicidad causada por metales pesados presentes en agua del río Cauca en la ciudad de Cali, Colombia

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    Aura H. Vivas

    2015-09-01

    Full Text Available Se evaluó la mutagenicidad del agua delrío Cauca debida a la presencia de metalespesados en la zona urbana de la Ciudadde Santiago de Cali, a partir de muestrastomadas en la temporada seca y lluviosaen el año 2013. Los metales se extrajeronpasando el agua por la resina AmberliteXAD-16. Las concentraciones de los metalespesados se midieron por absorción atómicay la mutagenicidad se evaluó por mediodel test de Ames, con las cepas TA98 yTA100 de Salmonella typhimurium con ysin activador enzimático S9. Los resultadosmostraron índices de mutagenicidad (IMpositivos (IM > 2,0 para muestras colectadasen temporada lluviosa en tres de los cincopuntos evaluados: puente El Hormiguero(IM = 3,6, desembocadura del CanalColector Sur (IM = 2,9 y desembocaduradel río Cali (IM = 2,7, todos con la cepaTA98 sin S9. Estos sitios presentaron a suvez las mayores concentraciones totales demetales pesados en sus extractos. El análisisde la variación espacio-temporal del índicemutagénico se realizó haciendo un análisis devarianza multifactorial del IM. Los resultadosencontrados indican que la época demuestreo contribuye significativamente a lavariabilidad del IM , mientras que los puntosde muestreo no.

  11. Reação de cultivares de batata à podridão mole causada por Pectobacterium carotovorum subsp. atrosepticum, por P. carotovorum subsp. carotovorum e por P. chrysanthemi Reaction of potato cultivars to soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, P. carotovorum subsp. carotovorum and P. chrysanthemi

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    Adilce I. H. Benelli

    2004-04-01

    Full Text Available A podridão mole em tubérculos de batata (Solanum tuberosum, causada por Pectobacterium carotovorum subsp. atrosepticum, por Pectobacterium carotovorum subsp. carotovorum e por P. chrysanthemi, é uma preocupante doença que causa danos expressivos à cultura em todo o mundo. Como inexiste tratamento eficiente para a podridão mole, o desenvolvimento de cultivares resistentes é considerado o método mais eficaz para a redução de perdas causadas pela doença. Nesse sentido, quatro cultivares de batata foram avaliados quanto à resistência natural às pectobactérias, mediante redução de massa de tubérculos após 20, 24, 48, 72 e 96 h de inoculação com suspensões bacterianas. O delineamento experimental constou de um esquema fatorial com quatro cultivares, três bactérias e quatro repetições. Os resultados foram transformados em proporção e integralizados como área abaixo da curva de progresso da doença (AACPD. Para as três bactérias estudadas, a cultivar Asterix mostrou-se o menos suscetível à podridão mole, diferindo significativamente dos demais.Soft rot caused by Pectobacterium carotovorum subsp. atrosepticum, Pectobacterium carotovorum subsp. carotovorum and P. chrysanthemi is a severe disease of potato (Solanum tuberosum worldwide. Since there is no efficient treatment for soft rot, the use of resistant cultivars is considered the most effective method to reduce crop losses due to this disease. In this research, four potato cultivars were evaluated regarding their resistance to pectobacteria. Tubers inoculated with bacterial suspensions were assessed for mass reduction at five periods (20, 24, 48, 72, and 96 h after inoculation. The experimental design consisted of a factorial scheme with four cultivars, three bacteria, and four replications. The data on tuber mass loss were transformed into proportion and integrated as the area under the disease progress curve. For all three bacteria, the cultivar Asterix was the less

  12. Endoftalmite por Candida albicans após transplante penetrante de córnea: relato de caso Candida albicans endophthalmitis following penetrating corneal graft: case report

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    Glaucio de Godoy

    2004-04-01

    Full Text Available Os autores relatam o caso de uma paciente submetida a transplante penetrante de córnea por ceratocone que evoluiu com resposta inflamatória exacerbada durante o período pós-operatório, suspeitando-se, inicialmente, de rejeição atípica e intensa ao botão doador. Houve períodos de melhora, seguidos por períodos de piora a cada tentativa de se reduzir o corticóide tópico. Iniciou-se terapia imunossupressora com ciclosporina via oral e altas doses de corticóide tópico, porém, a paciente desenvolveu endoftalmite por Candida albicans com acometimento do segmento anterior do olho e do corpo vítreo, mas sem surgimento de lesões coriorretinianas. Evoluiu de forma satisfatória após três injeções de anfotericina B 5µg intravítrea, cetoconazol 400 mg/dia via oral e clotrimazol tópico de 4/4 horas. Os autores discutem também as principais possibilidades de contaminação da paciente, sendo o quadro infeccioso provavelmente relacionado ao procedimento cirúrgico.The authors report a case of a patient submitted to penetrating corneal graft for keratoconus with an exaggerated inflammatory response during the postoperative period. The patient was suspected of having an atypical rejection to the donor button. There were periods of improvement, followed by periods of worsening of the inflammation at each attempt of reducing the topical corticosteroid. After initiating immunosuppressive therapy with cyclosporin and high doses of topical corticosteroid, the patient developed Candida albicans endophthalmitis affecting the anterior segment of the eye and the vitreous body, but sparing the chorioretina. The patient improved after three injections of 5 µg intravitreal amphotericin B, 400 mg oral ketoconazole daily and topical clotrimazole every four hours. The authors also discuss the main possibilities of contamination of the patient, the infectious picture probably being related to the surgical procedure.

  13. Anemia hemolítica causada por Indigofera suffruticosa (Leg. Papilionoideae em bovinos Hemolytic anemia caused by Indigofera suffruticosa (Leg. Papilionoideae in cattle

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    José Diomedes Barbosa Neto

    2001-03-01

    Full Text Available As partes aéreas de Indigofera suffruticosa Mill. (família Leg. Papilionoideae, planta incriminada pelos criadores de diversas áreas do Nordeste por doença caracterizada por hemoglobinúria em bovinos, foram administradas por via oral a seis bovinos, em doses diárias repetidas de 10 a 40 g/kg, Todos os animais experimentais apresentaram hemoglobinúria, porém passageira, apesar continuidade da administração da planta. Dois desses bovinos não apresentaram manifestações adicionais, um terceiro animal evidenciou manifestações leves, e os três outros, sintomas adicionais de intensidade moderada: apatia, mucosas visíveis de coloração esbranquiçada, pêlos arrepiados, anorexia, diminuição da freqüência e intensidade dos movimentos ruminais, taquicardia, pulso venoso positivo e dispnéia. Antes da crise hemolítica a urina apresentava coloração verde azulada. Nenhum animal experimental morreu, porém um foi sacrificado durante a fase hemoglobinúrica. À necropsia observaram-se anemia, bexiga contendo urina cor de vinho tinto, rins aumentados de volume com coloração marrom-escura, fígado, na superfície e ao corte, de coloração azulada com lobulação perceptível. As principais alterações histológicas foram verificadas no fígado, sob forma de necrose coagulativa e tumefação e/ou microvacuolização citoplasmática dos hepatócitos, e no rim representadas por acentuada nefrose, associada a grande quantidade de filtrado e/ou hemoglobina nos espaços de Bowman dentro de túbulos e do citoplasma das células epiteliais.The aereal parts of Indigofera suffruticosa Mill. (family Leg. Papilionoideae were force-fed fresh to 6 bovines in daily doses of 10 to 40 g/kg. Cattle breeders of various parts of the Northeast of Brazil accuse this plant as the cause of a non-fatal disease characterized by hemoglobinuria. The disease occurs only in years when the plant proliferates well invading the native pastures. All experimental

  14. Tumorogênese em plantas causadas por espécies de Agrobacterium Tumorigenesis in plants induced by species of Agrobacterium

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    Reginaldo da Silva Romeiro; José Roberto Vieira Júnior; Sérgio Hermínio Brommonschenkel

    2007-01-01

    Tumores - sintomas hiperplásicos em plantas - incitados por espécies de Agrobacterium sp. sempre exerceram fascínio sobre fitopatologistas desde o início do Século XX, quando Erwin Smith e colaboradores demonstraram serem eles de etiologia bacteriana. No início, imaginava-se que os tumores eram decorrentes de alterações hormonais na planta provocadas pela bactéria. Contudo, até recentemente, a microbiologia e a biologia molecular não eram suficientemente avançadas para que os cientistas pudes...

  15. Tizón foliar de la teca en vivero causada por Ralstonia solanacearum Biovar 4 en Tabasco, México

    OpenAIRE

    Omar Alejandro Pérez Vera; Silvia Edith García Díaz; Leopold Fucikovsky-Zak; Oscar Hernández Colula; José Tulio Méndez Montiel

    2013-01-01

    La teca (Tectona grandis) se utiliza en plantaciones comerciales por su rápido crecimiento y la alta calidad de su madera. En Huimanguillo, Tabasco, México, en 2009, se detectó una enfermedad en plantas de teca que provocó daños estimados de 30% de los viveros con dicha especie. Los síntomas característicos de esta enfermedad son marchitez y atizonamiento del follaje. De hojas se aisló una bacteria, se purificó y se inoculó en la planta de teca de cinco meses de edad; hubo marchitez, necrosis...

  16. Thermolysed and active yeast to reduce the toxicity of aflatoxin Formas termolisada e viva de leveduras na redução de toxicidade causada por aflatoxinas

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    Antonio Sampaio Baptista

    2002-06-01

    Full Text Available Aflatoxins are hepatotoxic metabolites produced by Aspergillus flavus and A. parasiticus on a number of agricultural commodities. This research was carried out to evaluate the ability of thermolysed and active Saccharomyces cerevisiae to attenuate liver damage caused by aflatoxin. Diets were prepared containing 0 aflatoxin; 400 mug kg-1 aflatoxin; 400 mug kg-1 aflatoxin plus 1% of dehydrated active yeast, and 400 mug kg-1 aflatoxin plus 1% of thermolysed yeast. A bioassay with Wistar rats was conducted for 28 days, and body organs were weighted and analyses of the liver tissue of the animals were performed. The relative weight of heart, kidneys and liver from animals submitted to the different treatments did not show any difference, and liver tissue of animals feeding on the aflatoxin-free diet was adopted as a toxicity-free pattern. Hepatic tissue of animals feeding on diets containing 400 mug kg-1 aflatoxin or the diet supplemented with 1% thermolysed yeast showed clear signs of toxicity and damage. Hepatic tissue of animals feeding on the diet containing 1% of dehydrated active yeast showed less toxicity signs and damage than those receiving the diet containing 400 mug kg-1 aflatoxin. Active, dehydrated yeast had the ability to reduce toxic effects caused by aflatoxin, but thermolysed yeast was not able to alleviate the effects of aflatoxin toxicity.As aflatoxinas são metabólitos hepatotóxicos produzidos por algumas linhagens de Aspergillus flavus, A. parasiticus e, eventualmente, por A. nomius sobre grande número de produtos agrícolas. Esta pesquisa foi conduzida para avaliar a capacidade de Saccharomyces cerevisiae, nas formas termolisada e desidratada viva, em reduzir os danos causados por aflatoxinas. Para tal, foi preparada uma dieta básica e desta se obtiveram quatro formulações: uma como controle; as demais contaminadas com aflatoxinas na concentração de 400 mig kg-1, sendo duas com posterior adição de 1% de leveduras, uma

  17. Resistência de clones e híbridos de porta-enxertos de citros à gomose de tronco causada por Phytophthora parasitica

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    Medina Filho Herculano P.

    2003-01-01

    Full Text Available Sete grupos de híbridos entre porta-enxertos elite de citros (Citrus sp. e seus genitores foram estudados quanto a reação à infecção de tronco por Phytophthora parasitica, em plantas adultas no campo. Inocularam-se artificialmente, em duas posições do tronco, 132 plantas nucelares dos genitores e 486 híbridos entre limão (Citrus limonia Cravo 'Limeira' (C, Poncirus trifoliata 'Davis A' (T, tangerina (C.sunki 'Sunki' (S e laranja Azeda (C. aurantium 'São Paulo' (A. A classificação do grau de resistência à gomose de tronco em plantas adultas no campo foi possível somente quando baseada na média do tamanho de lesões de mais de 30 plantas nucelares, devido às grandes variações observadas em plantas individuais do mesmo clone, impossibilitando a seleção precoce de híbridos baseada em valores de plantas individuais. O trifoliata e a laranja Azeda mostraram-se bastante resistentes, desenvolvendo, em geral, lesões de tamanho reduzido. A tangerina Sunki e o limão Cravo desenvolveram lesões maiores, embora a tangerina Sunki tenha mostrado uma tendência em desenvolver lesões maiores que às do limão Cravo. Híbridos de trifoliata apresentaram, no geral, lesões intermediárias. Os híbridos de Azeda apresentaram lesões de tamanhos bastante variáveis, porém a maioria, com lesões grandes. Da mesma forma comportaram-se os híbridos recíprocos entre Sunki e Cravo.

  18. Mortality of the outbreak defoliator Ormiscodes amphimone (Lepidoptera: Saturniidae caused by natural enemies in northwestern Patagonia, Argentina Mortalidad del defoliador epidémico Ormiscodes amphimone (Lepidoptera: Saturniidae causada por enemigos naturales en el noroeste de la Patagonia Argentina

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    JUAN PARITSIS

    2012-03-01

    Full Text Available Outbreaks of the defoliator moth Ormiscodes amphimone are occurring more frequently in numerous Nothofagus forests of Patagonia. However, little is known about the life history of this species including its natural enemies as mortality agents. In this work we quantified mortality by parasitoids and generalist predators at the egg, larval and pupal life stages of O. amphimone in a Nothofagus pumilio (Poepp. & Endl. Krasser forest in northwestern Patagonia. Parasitism of eggs was relatively low (ca. 11 %, and we did not record significant larval predation by insectivorous birds. However, we recorded elevated mortality caused by larval parasitoids (ca. 50 % in third instar larvae and pupal predators (ca. 75 %, which suggests that these natural enemies could play a significant role in regulating O. amphimone populations. Our research is an initial step towards understanding the influence of natural enemies on O. amphimone population dynamics.Las irrupciones poblacionales de la polilla defoliadora Ormiscodes amphimone están ocurriendo de manera más frecuente en numerosos bosques de Nothofagus de la Patagonia. Sin embargo, se sabe poco de la historia natural de esta especie y, particularmente, de sus enemigos naturales. En este estudio se cuantificó la depredación de huevos, larvas y pupas de O. amphimone por parasitoides y otros depredadores en un bosque de Nothofagus pumilio (Poepp. & Endl. Krasser en el noroeste de la Patagonia. Se encontró que el parasitismo de huevos fue relativamente bajo (ca. 11 % y no se registró depredación significativa de larvas por aves insectívoras. No obstante, registramos niveles elevados de mortalidad causada por parasitoides de larvas (ca. 50 % en larvas en tercer instar y por depredadores de pupas (ca. 75 %, lo que parece indicar que estos enemigos naturales desempeñan un papel significativo en la regulación de poblaciones de O. amphimone. Este estudio representa un paso inicial hacia el entendimiento de la

  19. Tratamento das ulcerações traumáticas bucais causadas por aparelhos ortodônticos Treatment of the mouth traumatic ulcers caused by orthodontic appliances

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    Antonio Adilson Soares de Lima

    2005-10-01

    Full Text Available As lesões de aspecto ulcerado na mucosa bucal são um achado comum na clínica odontológica e geralmente são ocasionados por trauma mecânico devido a próteses mal adaptadas, aparelhos ortodônticos e dentes com coroas ou restaurações fraturadas. Contudo, os indivíduos nos quais a lesão persiste por mais de três semanas deverão ser submetidos à biopsia ou outros exames para excluir algumas lesões de caráter neoplásico ou outras condições, tais como as infecções crônicas e as doenças de caráter imunológico. Durante o tratamento ortodôntico, a dor e o desconforto da mucosa bucal podem acontecer como resultado do traumatismo mecânico dos aparelhos, ocasionado pela fricção aumentada entre o tecido da mucosa e a superfície dos braquetes. Atualmente, o ortodontista possui poucos recursos para prevenir ou aliviar esta irritação na mucosa. O objetivo deste artigo é orientar o ortodontista para o manejo mais adequado destas lesões visando o seu diagnóstico correto, o alívio dos sintomas que estas costumam provocar e o tratamento mais adequado para cada tipo de caso.Mouth ulcers are one of the most commom complaints in the dental clinic. They are, in general, caused for mechanic traumatism induced by non-fitted dentures, orthodontic appliances and fracturated crowns and fillings. Nevertheless, those lesions that have persisted for more than three weeks should be submitted to biopsy or other exams to exclude neoplasic, infectious or immunologic lesions. During the orthodontic treatment, pain and soreness of the oral mucosa can occur as the result of mechanic traumatism caused by increased friction between soft tissues and brackets. Nowadays, the professional owns few resources to prevention or relieve this irritation on the oral mucosa. The aim of this article is to guide the orthodontist to realize the correct diagnoses of the mouth ulcers, the relieve the symptoms and the exact treatment for each type of case.

  20. Leishmaniose tegumentar americana causada por Leishmania (Viannia braziliensis, em área de treinamento militar na Zona da Mata de Pernambuco

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    Andrade Maria S.

    2005-01-01

    Full Text Available Este estudo tem como objetivo geral caracterizar a epidemiologia da leishmaniose tegumentar americana em unidade de treinamento militar, localizada no Estado de Pernambuco. Entre 2002 e 2003, vinte e três casos foram diagnosticados através de exame clínico, detecção do parasita e teste de intradermoarreação de Montenegro. Sete amostras de Leishmania (Viannia braziliensis foram isoladas destes pacientes, identificadas através de reações com anticorpos monoclonais específicos e perfil eletroforético com isoenzimas. Um inquérito epidemiológico de prevalência da infecção por IDRM foi realizado na população que realizou treinamento neste período, no qual foi identificada uma prevalência de 25,3% de infecção. Os dados obtidos, associados com achados prévios nesta área, apresentam evidências da manutenção de um ciclo enzoótico, com a ocorrência de surtos periódicos de leishmaniose tegumentar americana posteriormente à realização de treinamentos nas áreas de floresta Atlântica remanescente.

  1. ALTERACIONES HISTOPATOLOGICAS EN Lymnaea columella (PULMONATA: LYMNAEIDAE, HUESPED INTERMEDIARIO DE Fasciola hepatica (LINNEAEUS, 1758 (TREMATODA: FASCIOLIDAE, CAUSADAS POR Euphorbia splendens var. hislopii N.E.B.

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    EDWIN PILE

    1998-07-01

    Full Text Available RESUMEN El trabajo registra los cambios histopatológicos en Lymnaea columella huésped intermediario de Fasciola hepatica causados por el látex de Euphorbia splendens. Los caracoles fueron expuestos al látex en períodos diferentes siendo posteriormente procesados através de técnicas tradicionales. Los resultados demostraron necrosis en la glándula digestiva y gónadas ocurriendo pocas horas después del contacto con el productoHISTOPATHOLOGIC CHANGES IN Lymnaea columella (SAY, 1817 (PULMONATA: LYMNAEIDAE, INTERMEDIARY HOST OF Fasciola hepatica (LINNAEUS, 1758 (TREMATODA: FASCIOLIDAE, CAUSED FOR Euphorbia splendens var. hislopii N.E.B. The paper registers the histopathologic changes in Lymnaea columella intermediary host of Fasciola hepatica caused by the latex of Euphorbia splendens. The snails were exposed to the latex in different period being processed later on through of traditional techniques. The results demonstrated necrosis in the digestive gland and gonads happening few hours after the contact with the product

  2. Ulceração de anastomose esôfago-entérica causada por alendronato Esophagus-enteric anastomosis ulceration caused by alendronate

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    Pedro DUQUES

    2001-04-01

    Full Text Available Introdução — O alendronato de sódio, um aminobifosfonado indicado para o tratamento da osteoporose em mulheres pós-menopausadas, tem sido associado em vários relatos à esofagite. Estenose esofágica e sintomas gastrointestinais como dispepsia, náuseas, vômitos e dor abdominal também podem estar presentes. Objetivo — Relato do caso de paciente submetida a gastrectomia total com anastomose em Y de Roux devido a carcinóide gástrico que desenvolveu ulceração em anastomose esôfago-entérica após tratamento com esta droga. Material e Método — Paciente de 63 anos de idade, sexo feminino, iniciou terapia com alendronato na dose diária de 10 mg pela manhã, vindo a apresentar sintomas disfágicos e dor abdominal um mês após. Submetida a exame endoscópico, demonstrou-se ulceração no esôfago, na mucosa da anastomose esôfago-entérica e estenose esofágica. O tratamento medicamentoso foi descontinuado, tendo o sintoma de dor abdominal desaparecido e a disfagia diminuído de intensidade. Resultados — Houve remissão das lesões ulceradas, entretanto a estenose esofágica permaneceu. A paciente subseqüentemente foi tratada por dilatação endoscópica, evoluindo com melhora clínica e estando atualmente livre de sintomas. Conclusão — O alendronato de sódio tanto pode causar lesões na porção inferior do esôfago, como em segmentos mais distais do tubo digestivo nos pacientes cujo trânsito gastrointestinal é acelerado. Especial atenção deve ser dada aos pacientes gastrectomizados que fazem uso desta droga, pela possibilidade do desenvolvimento de lesões na mucosa entérica anastomosada e do surgimento de complicações temíveis como a estenose.Background — Alendronate sodium is an aminobisphosphonate indicated for the treatment of osteoporosis in post-menopausal women and has been associated with esophagitis in many reports. Esophageal stenosis, gastrointestinal symptoms as dyspepsia, nausea, vomiting and abdominal pain

  3. Mejoramiento Genético de la Papa en Colombia, para Resistencia a la "Gota" Causada por el PhytophthoraInfestans, (Mont. de Bary

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    Estrada Ramos Nelson

    1954-06-01

    Full Text Available Se hace una revisión de la literatura más importante y reciente publicada en EE. UU., Inglaterra, Holanda, Alemania y Rusia, países que más sehan preocupado por resolver el problema del Phytophthora en la papa, y la relacionada especialmente con el aspecto de pérdidas que causa, razas del parásito, métodos genéticos para la obtención de variedades resistentes y sistemas de prueba de la resistencia. Además se incluyen datos estadísticos para demostrar la importancia del Cultivo en el país. Se indican los métodos seguidos en Colombia para el mejoramiento, partiendo especialmente de líneas de la especie silvestre. S. demíssumcombinándola con las variedades nativas cultivadas del tipo andigenumadaptadas a grandes alturas y a los días cortos de los trópicos. Los resultados hasta la fecha indican la posibilidad de obtener después de 3 ó4 generaciones de retrocruzamientos, una buena variedad para cultivo y altamente resistente a la enfermedad. También se aconseja el empleo de líneas y variedades extranjeras resistentes, una vez que se hayan probado a las razas de Phytophthorapropias de Colombia. . Se indica que ya existe material de fitomejoramiento bastante avanzado que es prácticamente inmune pero que requiere subsiguiente mejoramiento. Hay probabilidades así, de obtener variedades inmunes o altamente resistentes a las razas actualmente existentes aunque su identidad parece no corresponder exactamente con las de otros países como Holanda, Inglaterra y Estados Unidos. Se establece una falta de identidad entre las pruebas de campo y las del laboratorio aunque pueden complementarse. Puede suponerse, de acuerdo con las reacciones obtenidas en el material extranjero resistente, la posibilidad de existencia de varias razas, pero no tan virulentas como en los países que tienen variedades resistentes. Es aconsejable el mejoramiento permanente de las variedades, para poder combatir los nuevos biotipos del pat

  4. AVALIAÇÃO DE PERDAS CAUSADAS POR Oebalus spp. (HEMIPTERA: PENTATOMIDAE EM ARROZ DE TERRAS ALTAS EVALUATION OF UPLAND RICE LOSSES CAUSED BY Oebalus spp. (HEMIPTERA: PENTATOMIDAE

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    Daniela Rézio e Silva

    2007-09-01

    Full Text Available

    Para determinar o efeito dos percevejos Oebalus poecilus e O. ypsilongriseus sobre o rendimento e a qualidade dos grãos de arroz de terras altas, panículas de cinco cultivares foram infestadas, isoladamente, pelos seguintes níveis populacionais: zero (tratamento testemunha, dois insetos adultos da primeira espécie, dois insetos adultos da segunda espécie e um inseto adulto de cada espécie. A comparação das cultivares pelas porcentagens de perda de massa por espigueta e de espiguetas vazias não apresentou efeitos diferenciados entre tratamentos, nem interação "tratamentos x cultivares" significativa. A avaliação da atividade alimentar, pela presença de bainhas de estilete, mostrou que o percevejo O. ypsilongriseus foi mais ativo que O. poecilus. Esses afetaram de modo diferente o vigor das cultivares, entretanto, não influenciaram o teor de amilose. Os percevejos também reduziram o rendimento total de arroz beneficiado e aumentaram o número de grãos inteiros manchados, apesar de não terem alterado o número total de grãos inteiros.

    PALAVRAS-CHAVE: Inseto; percevejo das panículas; bainha de estilete.

    To determine the effect of Oebalus poecilus and O. ypsilongriseus on grain yield and quality of five upland rice cultivars, isolated panicles were subjected to four levels of infestation (number of adult insects: zero (control treatment, two O. poecilus, two O. ypsilongriseus and one O. poecilus plus one O. ypsilongriseus. Neither the treatments nor the interaction of the treatments with cultivars had significant effects on percentages of weight loss and empty spikelets. Insect activity, evaluated by the presence of feeding sheaths in the spikelets, demonstrated that O. ypsilongriseus was more active than O. poecilus. Plant vigor was also affected differently

  5. Tumorogênese em plantas causadas por espécies de Agrobacterium Tumorigenesis in plants induced by species of Agrobacterium

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    Reginaldo da Silva Romeiro

    2007-03-01

    Full Text Available Tumores - sintomas hiperplásicos em plantas - incitados por espécies de Agrobacterium sp. sempre exerceram fascínio sobre fitopatologistas desde o início do Século XX, quando Erwin Smith e colaboradores demonstraram serem eles de etiologia bacteriana. No início, imaginava-se que os tumores eram decorrentes de alterações hormonais na planta provocadas pela bactéria. Contudo, até recentemente, a microbiologia e a biologia molecular não eram suficientemente avançadas para que os cientistas pudessem compreender e deduzir a forma através da qual o patógeno incitava os tumores. Demorou quase um século para que se deslindassem os complexos mecanismos bioquímicos, genéticos e fi­siológicos através dos quais o patógeno transforma a planta, inserindo no genoma desta uma região de seu megaplasmídeo de modo a criar para si mesmo um nicho ecológico espe­cífico. Neste trabalho é apresentada uma súmula histórica da evolução do conhecimento a respeito, das características genômicas do plasmídeo Ti, dos eventos e requerimentos ati­nentes ao processo infectivo bem como é discutida a dinâmica da transformação da planta pelo patógeno.Tumors - the plant hyperplasia symptoms- - induced by species of Agrobacterium sp. have deeply impressed plant pathologists since early 20th Century when Erwin Smith and his co-workers demonstrated that such tumors had a bacterial etiology. Nevertheless, until recently the state of art of Microbiology and Molecular Biology was not developed enough for scientists to realize and to elucidate the complexes biochemical, genetic and physiologic mechanisms by which the pathogen transforms the plant by inserting a region of its own plasmid into the genome of the latter, creating an specific ecological niche for itself. In this paper its is showed a historical brief on the evolution of knowledge about the genomic characteristics of the Ti Plasmid, events and requirements needed for infection to take

  6. Patrón de alteraciones en la radiografía de tórax de niños hospitalizados por infección causada por virus influenza A (H1N1) Pattern of chest radiographic abnormalities in children hospitalized because of influenza H1N1 virus infection

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    KARLA MOËNNE B; XIMENA ORTEGA F; JAIME CORDERO T; ALBERTO VIDAL G; JUAN A ESCAFFI J

    2010-01-01

    El año 2009 aparecieron los primeros casos de influenza humana causada por virus influenza A H1N1, propagándose como pandemia. En nuestra institución se observó 88,5% de aumento en consultas de urgencia, adultos y niños; se diagnosticaron 10.048 pacientes como influenza A H1N1 (45,6% confirmación de laboratorio). La media de edad fue 13 años. Se hospitalizaron 59 niños (edad: 1 mes - 15 años 7 meses), 33 niñas y 26 niños. El tiempo promedio de hospitalización fue de 3,9 días; 9 pacientes requ...

  7. Avaliação lectino-histoquímica de fígado e rim de ovinos com fotossensibilização causada por Brachiaria decumbens

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    Samay Z.R. Costa

    2013-08-01

    Full Text Available Embora sejam as forrageiras mais importantes para a pecuária de corte (bovinocultura de corte no Brasil, em certas épocas ou condições, Brachiaria spp. podem ser tóxicas e causar surtos de fotossensibilização hepatógena que determinam significativas perdas econômicas. Animais que se alimentam em pastos de Brachiaria spp. comumente apresentam macrófagos espumosos isolados ou agrupados, além de cristais no interior de ductos biliares. Saponinas esteroidais têm sido identificadas nestes cristais e são responsabilizadas por lesar o fígado levando ao acúmulo de filoeritrina. Por vezes, imagens negativas desses cristais podem estar presentes no citoplasma de macrófagos espumosos. A patogênese de formação e o tipo de material armazenado nas células espumosas ainda são desconhecidos. A técnica de lectino-histoquímica visa auxiliar na detecção desses macrófagos e, consequentemente, no diagnóstico, além de identificar quais os resíduos de açúcares específicos que estão presentes no citoplasma das células espumosas. Assim, este trabalho teve por objetivo identificar quais lectinas são mais indicadas na detecção de saponinas esteroidais no fígado e rim de ovinos com fotossensibilização causada por Brachiaria decumbens. Fragmentos de fígado e rim de quinze ovinos, de ambos os sexos e idade variável, oriundos de Conceição do Mato Dentro, Minas Gerais, e um ovino mantido em pastagem livre de Brachiaria spp. foram avaliados pela técnica de lectino-histoquímica. Quatorze lectinas foram utilizadas (Con-A, DBA, SBA, PNA, SJA, RCA-I, UEA-I, WGA, SWGA, GSL, PSA, PHA-L, PHA-E e LCA. Verificou-se que, no fígado de ovinos com fotossensibilização provocada pela ingestão de Brachiaria decumbens, a lectina PNA apresentou especificidade e acentuada reatividade aos macrófagos espumosos, bem como especificidade e leve reatividade aos hepatócitos; a lectina WGA teve especificidade e moderada reatividade aos macr

  8. Mastite clinica caprina causada por Arcanobacterium pyogenes

    OpenAIRE

    F. Garino Jr; R.A.T. Matos; E.G Miranda Neto; J.N.N. Bernardino; E.D. Santos; G.M.N. Aguiar

    2012-01-01

    This paper reports the occurrence of goat mastitis due to Arcanobacterium pyogenes in Paraiba State - Brazil. The diagnosis was confirmed by clinical symptoms and microbiological culture. Amastectomywasperformedasclinical treatment.

  9. Mastite clinica caprina causada por Arcanobacterium pyogenes

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    F. Garino Jr

    2012-08-01

    Full Text Available This paper reports the occurrence of goat mastitis due to Arcanobacterium pyogenes in Paraiba State - Brazil. The diagnosis was confirmed by clinical symptoms and microbiological culture. Amastectomywasperformedasclinical treatment.

  10. Desarrollo de una metodología para el estudio de las inundaciones causadas por encharcamiento de agua de lluvia en zonas urbanas relativamente planas. Area de estudio: Montería (Colombia

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    Padilla Agamez, Manuel

    2015-06-01

    Full Text Available In this paper a methodology for the analysis of the relationship between rainfall and flooding caused by ponding of rainfall water in urban area of Monteria (Colombia is developed. Rainfall water excesses over the soil were estimated from field measurements of the soil infiltration capacity for different soil units of the studied zone. The results obtained are presented in form of statistical correlations for calculating water rainfall excesses over the soils and maps of susceptibility and vulnerability for floods. The maps were used for a brief socioeconomic discussion of the effects of flood events in the selected zone.En el presente trabajo se desarrolla una metodología para el análisis de la relación existente entre las precipitaciones y las inundaciones causadas por encharcamiento de agua de lluvia en la zona urbana de Montería (Colombia. Se realizaron mediciones en campo de la capacidad de infiltración de agua para diferentes unidades de suelos de la zona de estudio, con el fin de estimar los excesos de agua de lluvia sobre el suelo. Los resultados obtenidos se presentan en forma de correlaciones estadísticas para el cálculo de los excesos de agua de lluvia y mapas de susceptibilidad y vulnerabilidad a las inundaciones, mediante los cuales se hace una breve discusión socioeconómica de los efectos de esta clase de inundaciones en la zona de estudio. [fr] Dans le présent travail est analysé la relation existant entre les précipitations et des inondations par accumulation d eau pluie sur le sol dans la zone urbaine de Montería (Colombie. Avec la réalisation de mesures en champ de la capacité d’infiltration pour différents unities de sol de la zone d’étude, on a estimé les excès d’eau pluie sur le sol. Les résultats obtenus sont présentés sous la forme de corrélations statistiques pour le calcul des excès d’eau pluie et de cartes de susceptibilité et vulnérabilité aux inondations. Les cartes ont été employ

  11. Enzima convertidora frente a quimasa mastocitaria en la infiltración leucocitaria inducida por angiotensina-I : papel de fractalquina en la inflamación vascular causada por humo de tabaco

    OpenAIRE

    Company de la Calle, Chantal

    2011-01-01

    En la presente Tesis Doctoral se han abordado dos objetivos: el estudio de la contribución de la enzima convertidora (ECA) frente a la quimasa mastocitaria en la acumulación leucocitaria inducida por la síntesis endógena de Ang-II in vivo, y el estudio del papel de fractalquina (FRK) en la inflamación sistémica inducida por el extracto de humo de tabaco (EHT). La enzima convertidora de la angiotensina (ECA) es la principal enzima implicada en la formación de Ang-II a partir de Ang-I. Sin...

  12. Surtos epidêmicos de dermatite causada por mariposas do gênero Hylesia (Lepidóptera: Hemileucidae) no Estado de São Paulo, Brasil Epidemic outbreaks of dermatitis caused by Hylesia (Lepidoptera: Hemileucidae), in S. Paulo State, Brazil

    OpenAIRE

    Carmen Moreno Glasser; João Luiz Cardoso; Glória Cristina Carréri-Bruno; Maria de Fátima Domingos; Roberto Henrique Pinto Moraes; Ricardo Mário de Carvalho Ciaravolo

    1993-01-01

    Surtos epidêmicos de dermatite causada por Hylesia sp foram assinalados no litoral do Estado de São Paulo, no período de dezembro de 1989 a dezembro de 1991. O fenômeno assumiu sua maior intensidade em Bertioga, Município de Santos, onde foram registrados 612 casos. Foram atingidos outros 12 municípios da região, estimando-se que algumas centenas de casos procuraram os serviços de saúde locais. Na maioria dos casos verificou-se lesões eritemato-pápulo-pruriginosas, que regrediram em média de ...

  13. Mortalidad por bacteriemia causada por Escherichia coli y Klebsiella spp. productoras de beta lactamasas de espectro extendido: cohorte retrospectiva en un hospital de Lima, Perú Mortality caused by bacteremia Escherichia coli and Klebsiella spp. extended-spectrum beta-lactamase- producers: a retrospective cohort from a hospital in Lima, Peru

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    Diego Adrianzén

    2013-03-01

    Full Text Available Objetivos. Evaluar los factores asociados a la mortalidad causada por bacteriemias por Escherichia coli y Klebsiella spp. productoras de beta lactamasas de espectro extendido (BLEE. Materiales y Métodos. Se realizó un estudio de cohortes retrospectivo, que incluyó 85 pacientes mayores de 16 años con diagnóstico de bacteriemia por Escherichia coli o Klebsiella spp. hospitalizados entre 2006 y 2008 en el Hospital Nacional Cayetano Heredia. Las cohortes se clasificaron de acuerdo a la producción de BLEE según los resultados de los hemocultivos. Se evaluaron los factores asociados a la mortalidad cruda y atribuible empleando regresión de Poisson en un modelo multivariado, con lo que se obtuvo riesgos relativos ajustados (RRa. Además, se construyeron curvas de mortalidad. Resultados. Se encontró que el 35,3% de las bacteriemias fueron debidas a cepas productoras de BLEE. El análisis de la mortalidad cruda mostró una mayor mortalidad en el grupo de cepas productoras de BLEE (63,3%. El RRa fue de 1,5 (IC95%: 1,02-2,3. En el caso de mortalidad atribuible, la proporción también fue mayor (63,3%, el RRa fue de 1,9 (IC95%: 1,2-2,9. El uso de catéter venoso central fue otro factor asociado tanto a la mortalidad cruda (RRa= 2,4; IC95%: 1,2- 4,8 como a la mortalidad atribuible (RRa= 3,8; IC95%: 1,6-8,8. Conclusiones. La producción de BLEE es un factor de riesgo independiente para mortalidad por bacteriemia causada por E. coli y Klebsiella spp. Su presencia debe evaluarse tras la sospecha diagnóstica y la elaboración de la terapéutica inicial, lo que podría disminuir la mortalidad por esta causaObjectives. To evaluate the factors associated to mortality caused by bacteremia due to Escherichia coli and Klebsiella spp. producers of extended-spectrum beta-lactamase (ESBL. Materials and methods. We performed a retrospective cohort study, including 85 patients older than 16 and diagnosed with bacteremia by Escherichia coli or Klebsiella spp

  14. Resíduos orgânicos para o controle das doenças do feijoeiro causadas por Sclerotium rolfsii / Organic residues for the control of bean diseases caused by Sclerotium rolfsii

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    Vanessa Nataline Tomazeli

    2011-04-01

    Full Text Available A incorporação de matéria orgânica ao solo tem se mostrado eficiente para o controle de alguns fitopatógenos habitantes do solo. O efeito dessa prática agrícola, principalmente sobre patógenos formadores de escleródios, como Sclerotium rolfsii, ainda não é completamente conhecido. O experimento, em dois anos de cultivo, foi conduzido no campo experimental da Universidade Tecnológica Federal do Paraná (UTFPR, em parcelas de um metro quadrado, contendo solo infestado com 100 g por parcela do substrato (arroz em casca contendo o patógeno. Os tratamentos foram chorume de suínos, cama de aviário, repolho triturado e testemunha, apenas patógeno, com quatro repetições. A semeadura de 80 sementes de feijão por parcela foi efetuada após uma semana da aplicação dos respectivos tratamentos. A intensidade da doença foi avaliada pela emergência, tombamento, incidência e severidade da doença. Os fatores como atividade microbiana, níveis de fertilidade do solo, número e sobrevivência de escleródios e massa das plantas fresca foram avaliados no fim do cultivo. O composto orgânico, cama de aviário, apresentou uma maior redução da incidência e a severidade da doença e, consequentemente, o tombamento de plântulas. Porém, apesar de reduzir a intensidade da doença, não houve aumento da emergência nos dois primeiros cultivos. Esse efeito benéfico da cama-de-aviário (CA pode estar associado ao aumento da matéria orgânica do solo e, consequentemente, da atividade microbiana, principal responsável pela redução da patogenicidade do fungo. Como consequência ao impacto provocado pela incorporação de CA ao solo, houve aumento da massa fresca da planta e redução do número de escleródios. Assim, com base nesses resultados, pode-se concluir que a CA apresenta efeito supressivo sobre as doenças causadas por S. rolfsii.AbstractOrganic matter incorporation into the soil has proved effective for the control of some soil

  15. Cerebral aspergillosis due to Aspergillus fumigatus in AIDS patient: first culture - proven case reported in Brazil Aspergilosis cerebral causada por Aspergillus fumigatus en paciente con SIDA: primer reporte de caso demostrado por cultivo en Brasil

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    José E. Vidal

    2005-06-01

    Full Text Available Cerebral aspergillosis is a rare cause of brain expansive lesion in AIDS patients. We report the first culture-proven case of brain abscess due to Aspergillus fumigatus in a Brazilian AIDS patient. The patient, a 26 year-old male with human immunodeficiency virus (HIV infection and history of pulmonary tuberculosis and cerebral toxoplasmosis, had fever, cough, dyspnea, and two episodes of seizures. The brain computerized tomography (CT showed a bi-parietal and parasagittal hypodense lesion with peripheral enhancement, and significant mass effect. There was started anti-Toxoplasma treatment. Three weeks later, the patient presented mental confusion, and a new brain CT evidenced increase in the lesion. He underwent brain biopsy, draining 10 mL of purulent material. The direct mycological examination revealed septated and hyaline hyphae. There was started amphotericin B deoxycholate. The culture of the material demonstrated presence of the Aspergillus fumigatus. The following two months, the patient was submitted to three surgeries, with insertion of drainage catheter and administration of amphotericin B intralesional. Three months after hospital admission, his neurological condition suffered discrete changes. However, he died due to intrahospital pneumonia. Brain abscess caused by Aspergillus fumigatus must be considered in the differential diagnosis of the brain expansive lesions in AIDS patients in Brazil.La aspergilosis cerebral es una causa rara de lesión expansiva cerebral en pacientes con SIDA. Presentamos el primer reporte de un absceso cerebral causado por Aspergillus fumigatus en un paciente brasileño con SIDA. El paciente, de 26 años de edad, presentaba antecedentes de infección por el virus de la inmunodeficiencia humana (VIH, tuberculosis pulmonar y toxoplasmosis cerebral. Manifestó fiebre, tos, disnea y dos episódios de convulsiones. La tomografía computadorizada (TC demostró una lesión hipodensa parasagital y bi-parietal con

  16. Cytomegalovirus and other herpesviruses infections in heart and bone marrow transplant recipients Infecções causadas por citomegalovírus e outros vírus do grupo herpes em transplantados cardíacos e de medula óssea

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    Adriana Weinberg

    1990-10-01

    Full Text Available From January 1988 to January 1989 all the heart transplant and bone marrow recipients at the Instituto do Coração of the Hospital das Clínicas of the University of São Paulo Medical School were studied for the incidence and morbidity associated with herpesviruses infections after transplantation. Five bone marrow and 5 heart transplant recipients were followed for a mean of 4.2 months post-transplantation. All the patients were seropositive for cytomegalovirus (CMV before admission and 80% experienced one or more recurrences during the observation period. Of the 12 episodes of CMV infection, that were identified in this study, 83% were accompanied by clinical or laboratory abnormalities. However, there was only one case of severe disease. The overall incidence of infection for herpes simplex (HSV was 50%. Although most of HSV reactivations were oral or genital, one case of HSV hepatitis occurred. One of the 6 episodes of HSV infections that were treated with acyclovir showed an unsatisfactory response and was successfully managed with ganciclovir. All the individuals had anti-varicella zoster virus antibodies, but none of them developed infection. The study emphasizes the importance of active diagnostic surveillance of herpesvirus infections in transplant patients. Both CMV and HSV reactivations showed high incidence and important morbidity and thus, deserve prophylactic therapy.De janeiro de 1988 a janeiro de 1989 todos os pacientes submetidos a transplante de coração ou de medula óssea no Instituto do Coração do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo foram estudados quanto à incidência e morbidade das infecções pós-transplante causadas por vírus do grupo herpes. Cinco recipientes de medula óssea e 5 transplantados cardíacos foram observados por um período médio de 4.2 meses após o transplante. Todos os pacientes tinham sorologia positiva para citomegalovírus (CMV antes do transplante

  17. RESOLUCIÓN CINÉTICA DE (R,S)-MANDELATO DE METILO POR PREPARACIONES INMOVILIZADAS DE LIPASA DE Candida antarctica B KINETIC RESOLUTION OF (R,S)-METHYL MANDELATE BY IMMOBILIZED LIPASE PREPARATIONS FROM Candida antarctica B

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    Andrés ESCORCIA; Jenniffer CRUZ; Torres, Rodrigo; Ortiz, Claudia

    2011-01-01

    El desarrollo de métodos para la obtención de compuestos quirales constituye uno de los grandes desafíos de la química actual. En este contexto, la resolución cinética catalizada por lipasas representa una excelente alternativa. En medios homogéneos, estas enzimas presentan equilibrio entre dos conformaciones (abierta y cerrada), el cual es posible desplazar hacia la conformación abierta (forma activa) en presencia de soportes hidrofóbicos. En este trabajo, la lipasa de Candida antarctica B (...

  18. Dried powders of velvetbean and pine bark added to soil reduce Rhizoctonia solani-induced disease on soybean Pós secos de mucuna e casca de pinus adicionados ao solo reduzem a doença causada por Rhizoctonia solani em soja

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    Luiz E. B. Blum

    2006-06-01

    Full Text Available Diseases induced by Rhizoctonia solani, like damping-off and root and stem rot on soybean (Glycine max, are a serious problem around the world. The addition of some organic material to soil is an alternative control for these diseases. In this study, benzaldehyde and dried powders of kudzu (Pueraria lobata, velvetbean or mucuna (Mucuna deeringiana, and pine bark (Pinus spp. were used in an attempt to improve soybean plant growth and to reduce the disease R. solani (AG-4 causes on soybean. Benzaldehyde (0.1-0.4 mL/kg of soil and velvetbean (25-100 g/kg significantly (P As doenças em soja (Glycine max causadas por Rhizoctonia solani são um sério problema ao redor do mundo. A incorporação ao solo de resíduos orgânicos é uma alternativa para o controle destas doenças. Neste estudo, benzaldeido e pós-secos de kudzu (Pueraria lobata, mucuna (Mucuna deeringiana e casca de pinus (Pinus spp. foram usados com o objetivo de melhorar o crescimento de plantas de soja e de diminuir a doença causada por R. solani (AG-4. Benzaldehyde (0,1-0,4 mL/kg de solo e mucuna (25-100 g/kg reduziram significativamente (P < 0.05 o crescimento micelial de R. solani em experimentos de laboratório. Em experimentos conduzidos em casa de vegetação a porcentagem de plantas sobreviventes foi maior em solo com casca de pinus e mucuna (50-100 g/kg. Em solo tratado com kudzu (r²=0,91 ou mucuna (r²=0,94, houve tendência significativa em aumentar a massa fresca das plantas de soja. Em microparcelas de campo solos com mucuna (r²=0,85 ou com casca de pinus (r²=0,61 reduziram significativamente a quantidade de doença. A quantidade de Bacillus megaterium (r²=0,87 e Trichoderma hamatum (r²=0,92 e a hidrólise de diacetato fluoresceina (r²=0,91 foram maiores em solo com doses crescentes de mucuna, indicando uma maior atividade microbiana. Neste estudo conclui-se que pós-secos de mucuna e casca de pinus, incorporados ao solo, podem reduzir a doença causada por R

  19. Tratamiento opcional de la diarrea producida por Cándida albicans en el cerdito (Optional treatment of scout caused for Candida albicans in piglets

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    Montejo Cuenca, Emilio

    2007-11-01

    Full Text Available Resumen Con el objetivo de comparar la eficacia de los tratamientos con la tintura de la hojas de guayaba (Psidium guajava L., tintura de las flores de manzanilla (Chrysantellum americanum L y el Hefrotrin 120 en las diarreas producidas por Candida albicans en el cerdito. Se trataron 80 crías porcinas entre 7 y 33 días de nacidas, afectadas por diarreas producidas por Candida albicans. Los resultados se analizaron utilizándose una comparación de proporciones mediante un análisis de varianza para %. El agente etiológico se aisló en el 90% de las muestras de heces fecales antes del tratamiento y en 100% de las muestras de pienso enviadas al laboratorio. Las diarreas se caracterizaron por 72,5% de heces líquidas y amarillas. El 45% de los animales estaba deshidratado y el 90% con anorexia. Se demostró que el tratamiento con la tintura de las hojas de guayaba y con la tintura de las flores de manzanilla es efectivo en la terapéutica de los trastornos gastroentéricos originados por la Cándida albicans en el cerdo; se observaron resultados similares a los obtenidos con Hefrotrin 120. Abstract The objective of the survey was to compare the effectiveness of a treatment using guava tincture, manzanilla tincture and Hefrotrine-120. In diarrheas produced by Candida Albicans were treated eighty piglets from 7 to 33 days affected by this diarrhea. The results were analized using a proportion comparation by means a variance analisys as %. The etiologic agent was isolated in the 90% of the feces samples before treatment and in three feed samples send to the lab. The scoun was characterized for 72,5% of liquid and yellow feces. The 45% of the animal were dehydrated and the 90% with anorexia. It was shown that the treatment with guava leaves tincture and chamomile flowers was clearly effective in the therapy in the gastrointestinal disturbances caused for Candida albicans in swine. The results obtained were similar to the ones got by using Hefrotrin

  20. Líquido cefalorraqueano em infecção do sistema nervoso central por leveduras do gênero Candida: análise de 11 casos

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    J. A. Livramento

    1989-09-01

    Full Text Available Foram estudadas 44 amostras de liqüido cefalorraqueano (LCR de 11 pacientes em que foi possível fazer diagnóstico etiológico de comprometimento do sistema nervoso central (SNC por leveduras do gênero Candida. Todos os pacientes apresentavam fatores de risco para a afecção. Cinco apresentavam quadro de meningite crônica a esclarecer; dois, síndrome de imunodeficiência adquirida; dois, traumatismo crânioencefálico seguido de meningite crônica; um, coagulação intravascular disseminada seguida de septicemia; um, candi-díase sistêmica pós-transplante renal. O diagnóstico etiológico foi feito pelo exame do LCR, possibilitando em 9 o isolamento da levedura por crescimento em meio de cultura. Em dois o diagnóstico foi feito pela presença das leveduras ao exame direto e, em um deles, também pela positividade da reação de fixação de complemento para Candida realizada em três amostras sucessivas de LCR. Discute-se a importância das alterações encontradas no exame do LCR na candidíase do SNC.

  1. Actividad antifúngica del aceite esencial de Eugenia caryophyllata sobre cepas de Candida tropicalis de aislados clínicos

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    Juliana MOURA MENDES; Felipe Queiroga SARMENTO GUERRA; de Oliveira Pereira, Fillipe; Janiere PEREIRA de SOUSA; Vinicius NOGUEIRA TRAJANO; de Oliveira Lima, Edeltrudes

    2012-01-01

    La candidiasis es una infección fúngica oportunista causada por levaduras del género Candida. En Brasil, la especie C. tropicalis esta siendo aislada frecuentemente, es el segundo microorganismo más aislado después de C. albicans. La aparición de cepas resistentes a los antifúngicos convencionales ha aumentado la búsqueda de nuevas alternativas provenientes de productos naturales, especialmente los aceites esenciales. En este estudio se investigó la actividad de los aceites esenciales contra ...

  2. Ocorrência de nova doença do algodoeiro irrigado, no Brasil, causada por Sclerotinia sclerotiorum Occurrence of a new disease of irrigated cotton, in Brazil, caused by Sclerotinia sclerotiorum

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    Maria José D´avila Charchar

    1999-06-01

    Full Text Available Em 1996, uma nova doença causada pelo fungo Sclerotinia sclerotiorum (Lib. de Bary foi observada em algodoeiro (Gossypium hirsutum L., cultivar Deltapine, irrigado sob pivô central, em Paracatu, MG. Os sintomas apresentados foram murcha e podridão da haste, do pecíolo da folha e da maçã, além de serem observados no interior do capulho micélio branco e escleródios escuros do patógeno. O teste de patogenicidade foi efetuado em algodoeiro, nas cultivares Deltapine e IAC 22, e em feijoeiro e quiabeiro, aos 14 dias de idade. As plantas foram incubadas em alta umidade durante 48 horas, a 25ºC. Três dias após a inoculação, verificaram-se sintomas severos de murcha e necrose dos tecidos, de onde o patógeno foi reisolado, completando-se, assim, os postulados de Koch. Este é o primeiro relato da ocorrência natural de S. sclerotiorum em algodoeiro no Brasil.In 1996, a new disease caused by Sclerotinia sclerotiorum (Lib. de Bary was observed on cotton (Gossypium hirsutum L. Deltapine cultivar irrigated under central pivot at Paracatu, MG, in the Savana region of Central Brazil. The symptoms were wilt, necrosis and rot. White mycelium and black sclerotia developed inside the boll. The pathogenicity test was done with two-week old cotton seedling cultivars Deltapine and IAC 22 and bean and okra seedlings. The plants were incubated at high humidity for 48 hours at 25ºC. Three days after inoculation, severe symptoms of wilt and necrosis were observed. S. sclerotiorum was reisolated from the damaged plant tissues, and Koch´s postulates were completed. This is the first report of S. sclerotiorum natural occurrence on cotton in Brazil.

  3. Patrón de alteraciones en la radiografía de tórax de niños hospitalizados por infección causada por virus influenza A (H1N1 Pattern of chest radiographic abnormalities in children hospitalized because of influenza H1N1 virus infection

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    KARLA MOËNNE B

    2010-09-01

    Full Text Available El año 2009 aparecieron los primeros casos de influenza humana causada por virus influenza A H1N1, propagándose como pandemia. En nuestra institución se observó 88,5% de aumento en consultas de urgencia, adultos y niños; se diagnosticaron 10.048 pacientes como influenza A H1N1 (45,6% confirmación de laboratorio. La media de edad fue 13 años. Se hospitalizaron 59 niños (edad: 1 mes - 15 años 7 meses, 33 niñas y 26 niños. El tiempo promedio de hospitalización fue de 3,9 días; 9 pacientes requirieron UTIy 4 ventilación mecánica. No hubo mortalidad en esta serie. Se demostró sobreinfección por VRS (5, infección bacteriana (9 y Mycoplasma (1. El patrón radiológico predominante en los niños hospitalizados correspondió a compromiso intersticial (72% y el 28% presentó hiperinsuflación pulmonar. Los niños con infección bacteriana asociada presentaron mayoritariamente (78% patrones radiológicos mixtos y de relleno alveolar. El propósito de esta revisión es conocer los patrones radiológicos en los niños que requirieron hospitalización por infección virus influenza A H1N1 en nuestra institución, durante la epidemia del año recién pasado.The first cases of H1N1- type Influenza virus infection in humans were reported in 2009, and since then, it rapidly expanded and became pandemic. At that time, an 88.5% increase in emergency consultations was observed in our institution, including adults and children and H1N1- type Influenza virus infection was clinically diagnosed in 10,048patients, 45.6% of them with laboratory confirmation. A total of 59 child, 33 girls and 26 boys, aged between 1 month and 15.5 years old, needed hospitalization. The average of hospitalization time was 3.9 days, 9 patients required intensive unit care and 4 of them mechanical ventilation. No fatal cases were registered in this series. Associated infection was confirmed in 15 patients: VRS (5, bacterial (9 and Mycoplasma (1. The most frequent

  4. Produção de fatores de virulência in vitro por espécies patogênicas do gênero Candida Production of virulence factors in vitro by pathogenic species of the genus Candida

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    Kelly Cristina Ortolan Rörig

    2009-04-01

    Full Text Available Avaliou-se, in vitro, a capacidade de crescimento em 39ºC e 42ºC, a produção de enzimas hidrolíticas e a atividade hemolítica de 21 cepas clínicas e de referência de sete espécies de Candida spp, Candida dubliniensis e Candida krusei demonstraram menor potencial de virulência e Candida albicans maior.The growth capacity at 39ºC and 42ºC, production of hydrolytic enzymes and hemolytic activity of 21 clinical and reference strains of seven species of Candida spp were evaluated in vitro.Candida dubliniensis and Candida krusei demonstrated lower virulence potential and Candida albicans higher potential.

  5. Lama glama con signología y lesiones compatibles con paratuberculosis causadas por Mycobacterium avium subespecie avium Lama glama with signology and lesion compatible with paratuberculosis and injuries caused by Mycobacterium avium subspecies avium

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    M.C Jorge

    2008-06-01

    Full Text Available Los camélidos sudamericanos (CS incluyen cuatro especies, guanaco, vicuña, alpaca y llama (Lama glama. En Argentina las llamas eran consideradas fauna y actualmente ganado, revalorizando su carne, fibra, cueros y pieles, también son un medio de subsistencia. Los CS son susceptibles a las enfermedades ocasionadas por micobacterias. El diagnóstico presuntivo se realiza por los signos clínicos y los hallazgos de necropsia y se confirma por técnicas bacteriológicas, moleculares e histopatología. El objetivo de este trabajo es describir un caso clínico con signos compatibles de paratuberculosis y el diagnóstico de laboratorio en una llama en cautiverio perteneciente a un zoológico de Olavarría, Provincia de Buenos Aires. En la necropsia se observaron lesiones granulomatosas en yeyuno, íleon y linfonodos mesentéricos compatibles con paratuberculosis, en los frotis directos y en la histopatología se observaron bacilos ácido-alcohol resistentes en cluster. Se confirmó la presencia de Mycobacterium avium subespecie avium por bacteriología y por PCR fue detectada la IS1245 característica de este agente, no detectando la IS900 correspondiente a Mycobacterium avium subespecie paratuberculosis. Esto permitió arribar al diagnóstico etiológico, combinando técnicas, de un caso de enteritis granulomatosa en llamas causado por Mycobacterium avium subespecie avium con signología y lesiones compatibles con paratuberculosis.Guanaco, vicuña, alpaca and llama (Lama glama are also known as Sudamerican camelids (SC. In Argentina llama was considered non profitable wildlife specie but now it is considered a mean for surviving because their meat, wool, leather and skin is valuable. SC are susceptible hosts of mycobacterial infections. A presumptive diagnosis is based on clinical and necropsy findings and is confirmed with bacterial isolation, molecular identification and histopathology. The objective of this publication is to describe a clinical

  6. Resíduos orgânicos para o controle das doenças do feijoeiro causadas por Sclerotium rolfsii / Organic residues for the control of bean diseases caused by Sclerotium rolfsii

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    Vanessa Nataline Tomazeli; Idalmir Santos; Rafael Gustavo Ferreira Morales

    2011-01-01

    A incorporação de matéria orgânica ao solo tem se mostrado eficiente para o controle de alguns fitopatógenos habitantes do solo. O efeito dessa prática agrícola, principalmente sobre patógenos formadores de escleródios, como Sclerotium rolfsii, ainda não é completamente conhecido. O experimento, em dois anos de cultivo, foi conduzido no campo experimental da Universidade Tecnológica Federal do Paraná (UTFPR), em parcelas de um metro quadrado, contendo solo infestado com 100 g por parcela do s...

  7. Epidemiología de la babesiosis de los bovinos causada por Babesia bigemina (Smith y Kilborne, 1893) en el sudoeste de la provincia del Chaco

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    Mastropaolo, Mariano

    2014-01-01

    Babesia bigemina es uno de los agentes etiológicos de la babesiosis de los bovinos y en Argentina es transmitida solamente por ninfas y adultos de la garrapata Rhipicephalus (B.) microplus. Los objetivos de este trabajo fueron evaluar el estatus epidemiológico y factores asociados para B. bigemina en rodeos del sudoeste de la provincia del Chaco y evaluar la incidencia de infección y la proporción de garrapatas infectadas en dos establecimientos ganaderos (AT y VA) de la región. Se determinó ...

  8. Relación entre Condiciones de Suelo y Manejo de Huertas de Nogal Pecanero Carya illinoensis Koch con la Dinámica de la Pudrición Texana, causada por Phymatotrichum omnivorum Duggar

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    José Alfredo Samaniego Gaxiola; Teodoro Herrera Pérez; Aurelio Pedroza Sandoval; Jesús Santamaría César

    2001-01-01

    De 1995 a 1998, 26 lotes de tres ha cada uno fueron seleccionadas en huertas comerciales de nogal pecanero. El objetivo del muestreo fue relacionar la dinámica de la Pudrición Texana con el manejo de las huertas y condición de suelo. Las variables en el manejo del cultivo fueron: edad de los árboles, distancia de plantación, árboles por hectárea, manejo de plagas, número de riegos e incidencia de árboles enfermos (en 1995); para el factor suelo se consideraron tres profundidades (0-30, 30-60 ...

  9. Reações adversas causadas por fármacos que atuam no sistema nervoso: análise de registros de um centro de farmacovigilância do Brasil Adverse reaction caused by drugs acting in nervous system: records analysis of a farmacovigilance center in Brazil

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    Marta Maria de França Fonteles

    2009-01-01

    Full Text Available CONTEXTO: A morbimortalidade por uso de medicamentos é um grande problema de saúde. As reações adversas a medicamentos podem resultar em óbito, aumento de internações hospitalares e dos custos com a saúde. OBJETIVOS: Descrever e analisar as notificações de suspeitas de reações adversas causadas por medicamentos que atuam no sistema nervoso (RAM-SN, registradas no Centro de Farmacovigilância do Ceará, de janeiro de 1997 a março de 2008. MÉTODOS: As RAM-SN foram classificadas segundo os critérios da Organização Mundial da Saúde. Uma relação de causalidade entre o fármaco administrado e a reação adversa identificada foi realizada, bem como a análise da reação quanto à gravidade. RESULTADOS: Foram registradas 176 notificações de RAM-SN. A maioria (n = 145; 82,4% ocorreu no ambiente hospitalar. O principal notificador foi o farmacêutico. As RAM-SN foram classificadas como: possíveis (n = 110, prováveis (n = 37 e definidas (n = 17. Quanto à gravidade, foram consideradas: leves (n = 21, moderadas (n = 127, graves (n = 15 e fatais (n = 1. O caso fatal foi notificado por médico e envolveu medicamentos anestésicos. Geralmente, as reações adversas observadas foram causadas predominantemente por analgésicos, anestésicos e antiepilépticos. DISCUSSÃO: Os dados demonstram o valor potencial de se ter acesso a sistemas de farmacovigilância local para registrar possíveis riscos com o uso de fármacos.BACKGROUND: The morbi-mortality by the use of medicines is a major health problem. The drug adverse reactions may result in death, increased hospitalizations and healthcare costs. OBJECTIVES: Describe and analyze reports of suspected adverse reactions caused by drugs that act on the nervous system (SN-ADR, registered in the database of the Pharmacovigilance Centre of Ceará, from January 1997 to March 2008. METHODS: All the NS-ADRs were classified according to criteria of the World Health Organization. The causality

  10. Insuficiência respiratória aguda causada por pneumonia em organização secundária à terapia antineoplásica para linfoma não Hodgkin

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    Adriell Ramalho Santana; Fábio Ferreira Amorim; Paulo Henrique Alves Soares; Edmilson Bastos de Moura; Marcelo de Oliveira Maia

    2012-01-01

    Doenças difusas do parênquima pulmonar pertencem a um grupo de doenças de evolução geralmente subaguda ou crônica, mas que podem determinar insuficiência respiratória aguda. Paciente masculino, 37 anos, em terapia para linfoma não Hodgkin, admitido com tosse seca, febre, dispneia e insuficiência respiratória aguda hipoxêmica. Iniciadas ventilação mecânica e antibioticoterapia, porém houve evolução desfavorável. Tomografia computadorizada de tórax mostrava opacidades pulmonares em "vidro fosco...

  11. Meningoencefalite em bovinos causada por herpesvírus bovino-5 no Mato Grosso do Sul e São Paulo Meningoencephalitis in cattle caused by bovine herpesvirus-5 in Mato Grosso do Sul and São Paulo

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    Sandro César Salvador

    1998-04-01

    Full Text Available Quinze focos de meningoencefalite por herpesvírus bovino-5 (BHV-5 foram diagnosticados entre agosto de 1993 e dezembro de 1996, sendo 14 provenientes do estado do Mato Grosso do Sul e um do estado de São Paulo. A doença ocorreu em diversos municípios e em diferentes épocas do ano. Foram afetados bovinos de 6 a 60 meses de idade, com uma morbidade de 0,05% a 5% e letalidade próxima a 100%. Os sinais clínicos foram exclusivamente nervosos e o curso da enfermidade variou de 1 a 15 dias. As principais lesões histológicas detectadas foram meningite e encefalite difusa com malacia do córtex cerebral e presença de corpúsculos de inclusão intranucleares em astrócitos e neurônios. O vírus foi isolado do cérebro de 11 de um total de 12 animais, e sua identidade confirmada por imunoperoxidase, utilizando-se anticorpos monoclonais específicos. Os surtos de encefalite por BHV-5 representam 5% dos diagnósticos realizados em bovinos pelo Hospital Veterinário da Universidade Federal do Mato Grosso do Sul. Os resultados deste trabalho evidenciam a importância da doença no Mato Grosso do Sul e indicam a necessidade de incluir a encefalite por BHV-5 no diagnóstico diferencial de outras doenças do sistema nervoso de bovinos frequentes no Estado.Fifteen outbreaks of bovine herpesvirus-type 5 (BHV-5 infection were diagnosed from August 1993 to December 1996. Fourteen outbreaks occurred in the State of Mato Grosso do Sul and one in the State of São Paulo. Cattle 6 to 60 months old were affected. Morbidity reached 0.05% to 5% and case fatality rate was nearly 100%. The disease occurred in different municipalities and at different times of the year. Clinical signs were exclusively nervous, and the clinical course varied from 1 to 15 days. The main histologic lesions were meningitis, diffuse encephalitis and necrosis of the cerebral cortex with intranuclear inclusion bodies in astrocytes and neurons. BHV-5 was isolated from 11 out of 12 brains of

  12. Human pseudomyiasis caused by Eristalis tenax (Linnaeus (Diptera: Syrphidae in Goiás Pseudomiíase humana causada por Eristalis tenax (Linnaeus (Diptera: Syrphidae em Goiás

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    Marco Tulio A. Garcia-Zapata

    2005-04-01

    Full Text Available The objective of the present work was to register the first proven cases of human pseudomyiasis due to Eristalis tenax in Goiás State, Brazil, underscoring their clinical manifestations and direct relationship with hygiene. The taxonomic identification of the instars was done according to the descriptions and keys presented by James (1947, Hartley (1961 and Guimarães & Papavero (1999. Two cases were observed. In both cases there was no evidence of apparent mental disturbance. The clinical picture of these cases was self limited. The water supply, sewer system, socioeconomic level and habits of the suspect species of the flies are criteria that should be investigated.O presente trabalho visa registrar os primeiros casos evidenciados de pseudomiíases humanos por Eristalis tenax no estado de Goiás, Brasil, destacando suas manifestações clínicas e suas relações diretas com os hábitos higiênicos. A identificação taxonômica das larvas foi realizada com base nas descrições e chaves apresentadas por James (1947, Hartley (1961 e Guimarães & Papavero (1999. Observaram-se dois casos. Em ambos não havia evidência de pertubações mentais claras. O quadro clínico de ambos os casos era mesmo limitado. O abastecimento de água, o nível sócio-econômico e o hábito das espécies das moscas são critérios que devem ser considerados na investigação.

  13. Efeito do chorume líquido de suínos na podridão do colo e tombamento de plântulas de feijoeiro causadas por Sclerotium rolfsii Effect of liquid swine manure on collar rot and damping-off of bean plantlets caused by Sclerotium rolfsii

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    Rafael G.F. Morales

    2007-10-01

    Full Text Available O chorume líquido de suínos (CLS pode ser utilizado como fonte de nutrientes e de matéria orgânica para algumas culturas agrícolas e interferir na ocorrência de doenças de plantas, causadas por fitopatógenos habitantes do solo. Assim, foi estudado o efeito do CLS, sobre as doenças do feijoeiro (Phaseolus vulgaris causadas por Sclerotium rolfsii. Em parcelas de 1 m², foi incorporado o CLS nas doses equivalentes a 0, 20, 40, 60 e 80 m³ ha-1. O solo foi infestado dois meses antes da aplicação do CLS, com 100 g do substrato (arroz em casca colonizado pelo patógeno. A semeadura de 80 sementes de feijão por parcela foi efetuada em dois cultivos sucessivos, 1 dia e 45 dias após a aplicação do CLS. A intensidade da doença foi avaliada através da emergência, estande final de plântulas e severidade da doença, nos dois cultivos, sendo que a atividade microbiana, a concentração de amônia na camada superficial do solo e os níveis de fertilidade foram avaliados apenas no segundo cultivo. Com o aumento das doses de CLS foi verificada a redução da intensidade da doença e, entre as características avaliadas, o aumento da atividade microbiana, da concentração de amônia e dos níveis de cobre e zinco são os que melhor explicam essa redução.Liquid swine manure (LSM can be used as a source of nutrients and organic matter for some agricultural crops, and may interfere in plant diseases caused by soil-borne plant pathogens. Thus, the effect of LSM on bean (Phaseolus vulgaris diseases caused by Sclerotium rolfsii was studied. LSM was incorporated into 1 m² plots at doses equivalent to 0, 20, 40, 60, and 80 m³ ha-1. The soil was infested two months prior to LSM application with 100 g of substrate (unhulled rice colonized by the pathogen. Eighty bean seeds were sown per plot in two successive cultivations, 1 day and 45 days after LSM application. Intensity of the disease was evaluated based on plant emergence, final stand, and

  14. Epidemiology of extended spectrum β-lactamase producing Enterobacter bacteremia in a brazilian hospital Epidemiologia de bacteremia causadas por Enterobacter produtores de β-lactamases de espectro estendido em um hospital brasileiro

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    Felipe Francisco Tuon

    2010-08-01

    Full Text Available INTRODUCTION: Enterobacter can be included in the group of extended spectrum β-lactamases (EBSL-producing bacteria, though few studies exist evaluating risk factors associated with this microorganism. A retrospective cohort study was conducted to determine risk factors associated with ESBL-producing-Enterobacter and mortality METHODS: A retrospective cohort study with 58 bacteremia caused by ESBL-producing-Enterobacter (28 cases and non-ESBL (30 cases RESULTS: Risk factors associated with ESBL-Enterobacter were trauma, length of hospitalization, admission to the intensive care unit, urinary catheter and elective surgery (pINTRODUÇÃO: Enterobacter pode ser incluído no grupo de bactérias produtoras de β-lactamases de espectro estendido (ESBL, mas existem poucos estudos avaliando fatores de risco para ESBL. Nós realizamos uma coorte retrospective para determiner fatores de risco associados com Enterobacter produtores de ESBL MÉTODOS: Uma coorte retrospectiva com 58 bacteremias por Enterobacter ESBL (28 casos e não-ESBL (30 casos RESULTADOS: Fatores de risco para ESBL-Enterobacter foram trauma, tempo de internação, admissão em UTI, sonda vesical e cirurgia eletiva (p<0.05. A mortalidade foi similar entre ESBL e não-ESBL CONCLUSÕES: Enterobacter produtor de ESBL é prevalente e a curva de mortalidade foi semelhante com o grupo não-ESBL.

  15. Insuficiência respiratória aguda causada por pneumonia em organização secundária à terapia antineoplásica para linfoma não Hodgkin

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    Adriell Ramalho Santana

    2012-12-01

    Full Text Available Doenças difusas do parênquima pulmonar pertencem a um grupo de doenças de evolução geralmente subaguda ou crônica, mas que podem determinar insuficiência respiratória aguda. Paciente masculino, 37 anos, em terapia para linfoma não Hodgkin, admitido com tosse seca, febre, dispneia e insuficiência respiratória aguda hipoxêmica. Iniciadas ventilação mecânica e antibioticoterapia, porém houve evolução desfavorável. Tomografia computadorizada de tórax mostrava opacidades pulmonares em "vidro fosco" bilaterais. Devido ao paciente ter feito uso de três drogas relacionadas à pneumonia em organização (ciclofosfamida, doxorrubicina e rituximabe e quadros clínico e radiológico serem sugestivos, iniciou-se pulsoterapia com metilprednisolona com boa resposta. Pneumonia em organização pode ser idiopática ou associada a colagenoses, drogas e neoplasias, e geralmente responde bem a corticoterapia. O diagnóstico é anatomopatológico, mas condições clínicas do paciente não permitiam a realização de biópsia pulmonar. Pneumonia em organização deve ser diagnóstico diferencial em pacientes com aparente pneumonia de evolução desfavorável ao tratamento antimicrobiano.

  16. Impact of the Haemophilus influenzae type b vaccination program on HIB meningitis in Brazil Impacto do programa de vacinação contra meningites causadas por Haemophilus influenzae tipo b no Brasil

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    Sybelle de Souza Castro Miranzi

    2007-07-01

    Full Text Available This study aimed to evaluate the impact of vaccination against Haemophilus influenzae type b (HIB in Brazil on the morbidity, mortality, and case fatality of HIB meningitis, using the Ministry of Health database and population data from the Brazilian Institute of Geography and Statistics (Instituto Brasileiro de Geografia e Estatística - IBGE. Impact was evaluated through a time series analysis (1983-2002, using regression forecasting (RF by dividing the time series into two periods: (a historical (1983-1998 and (b validation (1999-2002. Impact of the vaccination was positive, although more significant for incidence and mortality than for case fatality rates.A proposta deste trabalho foi avaliar o impacto da vacinação contra Haemophilus influenzae tipo b (HIB no Brasil sobre a morbi-mortalidade e a letalidade das meningites por HIB, a partir de base de dados fornecida pelo Ministério da Saúde e as estimativas populacionais provenientes do Instituto Brasileiro de Geografia e Estatística (IBGE. Para a avaliação do impacto utilizou-se análise de tendência temporal (1983-2002, aplicando-se a técnica RF (regression forecasting, dividindo-se a série em dois períodos: (a período histórico (1983-1998 e (b período de estimação (1999-2002. O impacto da vacinação foi positivo, embora tenha se revelado mais expressivo sobre a morbi-mortalidade que sobre a letalidade.

  17. Alternativa de Convivencia con el Problema de la Salinidad del Agua de Irrigación causada por Presas Subterráneas Alternative of Coexistence with the Salinity Probiem of Irrigation Water caused by Underground Dams

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    José Wilmar  da S Neto

    2011-01-01

    Full Text Available Se presenta una alternativa de convivencia con el problema de salinidad del agua de irrigación causado por presas subterráneas. Se analiza la calidad del agua de irrigación comparando el crecimiento vegetativo de las gramíneas Capim Vetiver, Sempre Verde; Cana-de-agúcar, Capim Elefante cv. Napier y Capim Elefante cv. Mercker, aguas arriba y abajo de la presa subterránea localizada en el municipio de Ibicuitinga, estado de Ceará en Brasil. El análisis de muestras de aguas subterráneas aguas arriba y abajo de la presa han mostrado que ambas poseen un grado de salinidad elevado para fines de irrigación, recomendándose el uso de cultivos halófitos. Se ha realizado el análisis de varianza para variable crecimiento en altura y se ha aplicado la prueba de Tukey. A partir de esta prueba se ha verificado que, de entre las gramíneas probadas, tan sólo la Capim Elefante cv. Mercker ha diferido estadísticamente.The objective of this paper is to show an alternative for the coexistence with the salinity probiem of irrigation water caused by underground dams. The quality of irrigation water is analyzed by comparing the vegetative growth of Capim Vetiver, Sempre Verde, Cana-de-Acucar, Capim Elefante cv. Napier and Capim Elefante cv. Mercker, upstream and downstream of the underground dam located in Ibicuitinga, Ceará state in Brazil. The analysis of groundwater samples upstream and downstream of the dam have shown that both have high salinity for irrigation, recommending the use of halophytes crops. The study includes an analysis of variance for height growth variable and the Tukey test was applied. From this test it was verified that among the gramineous plants tested, only the Capim Elefante cv. Mercker was found statistically different.

  18. La marchitez tardía del maíz (Zea mays L. causada por Cephalosporium maydis en la Península Ibérica, y otros hongos asociados

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    Carmen Maria Ortiz-Bustos

    2015-06-01

    Full Text Available Las especies de hongos de suelo asociadas a Cephalosporium maydis como agente causal de la marchitez tardía del maíz en la Península Ibérica se identificaron muestreando 19 campos con síntomas de marchitez en las principales zonas de cultivo entre 2011 y 2012. En el 47% de los campos no se identificó C. maydis, pero sí Fusarium graminearum, F. verticillioides, F. equiseti, F. proliferatum, Macrophomina phaseolina, Rhizoctonia solani y Trichoderma harzianum infectando las plantas de maíz. En los campos restantes, junto a C. maydis se identificaron otros hongos de suelo en porcentajes apreciables: F. verticillioides (19%, F. proliferatum (19%, F.equiseti (9%, F. oxysporum (9% y Pythium oligandrum (9%. El crecimiento vascular de C. maydis y de otras especies fúngicas en plantas de maíz se confirmó analizando plantas con marchitez procedentes de tres campos diferentes. Tanto C. maydis como F. graminearum, F. equiseti, F. proliferatum y T. harzianum se aislaron de la inserción entre la raíz y tallo y a 10 cm de altura en el tallo de las plantas. El efecto de la infección por C. maydis sobre la producción de las plantas de maíz se cuantificó en macetas y condiciones seminaturales en el 2011. En plantas inoculadas se obtuvo una reducción del peso de las mazorcas del 54%, además de pesos de raíz y de parte aérea (tallo y hojas significativamente menores en comparación con el control no inoculado, lo que sugiere el gran impacto económico que puede tener la marchitez tardía en condiciones naturales. Asimismo este trabajo pone de manifiesto el grado de complejidad de la etiología de la marchitez tardía, que debería ser estudiado mediante la confirmación de la patogenicidad de los hongos de suelo identificados en maíz, con el fin de determinar el papel que puede jugar cada una de estas especies en el desarrollo de la enfermedad y/o severidad de los síntomas.

  19. Controle químico da queima de folhas e da mela de estacas de eucalipto, causadas por Rhizoctonia spp. Chemical control of leaf scorch and web blight of eucalypt cuttings, caused by Rhizoctonia spp.

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    Silvaldo Felipe da Silveira

    2003-12-01

    Full Text Available Visando ao controle químico da queima de folhas e mela de estacas de eucalipto (Eucalyptus spp. em viveiros florestais, avaliou-se a eficiência de 12 fungicidas em inibir in vitro o crescimento micelial de um isolado epifítico de Rhizoctonia solani AG1-IB (RH-2. Sete fungicidas que inibiram totalmente o crescimento micelial do fungo, a concentrações inferiores a 100 ppm, foram pré-selecionados: methyl-tolclophos, benomyl, pencycuron, iprodione, thiabendazol, thiram e captan. Avaliou-se, ainda, a sensibilidade (EC50 = dose provável que inibe o crescimento micelial em 50% aos fungicidas methyl-tolclophos, benomyl, iprodione e pencycuron de mais oito isolados patogênicos ao eucalipto, que diferem entre si quanto a virulência, morfologia, grupo de anastomose, número de núcleos por célula vegetativa e padrões eletroforéticos de proteínas e isoenzimas. Embora variações nos valores de EC50 entre algumas combinações de fungicidas e isolados tenham ocorrido, todos os isolados foram sensíveis aos quatro fungicidas testados (EC50 To achieve the chemical control of leaf scorch and web blight of eucalyptus (Eucalyptus spp. cuttings in forest nurseries, 12 fungicides were assayed against one isolate of Rhizoctonia solani AG1-IB (RH-2. Seven fungicides (methyl-tolclophos, benomyl, pencycuron, iprodione, thiabendazol and captan completely inhibited mycelial growth under concentrations lower than 100 ppm of active ingredient on PDA culture. Additionally, the sensibility (EC50 = estimated dose for 50% inhibition of mycelial growth to methyl-tolclophos, benomyl, pencycuron and iprodione of another eight isolates differing in virulence, morphology, anastomosis groups, and proteins and isozyme profiles was evaluated. Little differences in sensitivity was observed in some combinations of fungicides-isolates. However, the eight isolates were sensitive to the four tested fungicides (EC50 < 11 ppm. Under artificial conditions of inoculations, sprays

  20. CELULITIS FACIAL ODONTOGÉNICA SEVERA INFRECUENTE CAUSADA POR CITROBACTER FREUNDII PRODUCTORA DE AMPC EN UN PACIENTE CON DIABETES MELLITUS 2. REPORTE DE CASO CELULITE FACIAL ODONTOGÊNICA SEVERA INFREQUENTE CAUSADA POR CITROBACTER FREUNDII PRODUTORA DE AMPC EM UM PACIENTE COM DIABETES MELLITUS 2. RELATÓRIO DE CASO UNCOMMON SEVERE ODONTOGENIC FACIAL CELLULITIS CAUSED BY AMPCPRODUCING CITROBACTER FREUNDII IN A PATIENT WITH TYPE 2 DIABETES MELLITUS. CASE REPORT

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    CAMILO ESLAVA

    2012-06-01

    Full Text Available El manejo de la celulitis facial odontogénica no deja de ser un tema controversial en el campo de la cirugía oral y maxilofacial; los principios quirúrgicos y terapéuticos han sido sometidos a modificacio nes basadas en los hallazgos clínicos, imagenológicos y microbiológicos a través del tiempo. En pacientes con diabetes mellitus 2 se incrementa el riesgo a sufrir infecciones bacterianas oportunistas con tiempos de hospitalización más prolongados que la población no diabética. La literatura es clara estableciendo las diferencias clínicas y microbiológicas de la celulitis facial odontogénica en este grupo de pacientes, sin embargo, no existe un protocolo médico quirúrgico destinado a ellos. El microorganismo comúnmente aislado es Klebsiella pneumoniae, mientras Citrobacter freundii es inusual en las infecciones odontogénicas, su capacidad para producir betalactamasas de amplio espectro (AmpC le permite bloquear la acción de los antibióticos de uso empírico en Cirugía Oral y Maxilofacial. A continuación, presentamos el caso de una paciente de 61 años con diabetes Mellitus tipo 2 y celulitis facial odontogénica por Citrobacter freundii productora de AmpC.O tratamento da celulite facial odontogênica não deixa de ser um tema controverso no campo da Cirurgia Oral e Maxilofacial; os princípios cirúrgicos e terapêuticos foram submetidos a modificações baseadas nos descobrimentos clínicos, imagenológicos e microbiológicos através do tempo. Em pacientes com Diabetes Mellitus 2 aumenta o risco de sofrer infecções bacterianas oportunistas com tempos de hospitalização mais prolongados que na população não diabética. A literatura é clara estabelecendo as diferenças clínicas e microbiológicas da Celulite Facial Odontogênica neste grupo de pacientes; porém, não existe um protocolo médico cirúrgico destinado a eles. O microrganismo comunmente isolado é o Klebsiella pneumoniae, enquanto que o Citrobacter

  1. Efeitos da esplenectomia na peritonite causada por lesão traumática do cólon: estudo em ratos Effects of splenectomy on peritonitis produced by a colonic injury: study in rats

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    Luís Sérgio Nassif

    2004-09-01

    Full Text Available OBJETIVO: Estudar o efeito da esplenectomia na infecção intra-abdominal com bactérias da flora enteral, liberadas para a cavidade abdominal através de uma lesão induzida no cólon de ratos Wistar. MÉTODOS: Foram utilizados 64 animais, sendo 20 do Grupo A1 (normais sem sutura da lesão, 22 do Grupo A2 (normais com sutura da lesão e 22 do Grupo B (esplenectomizados e com sutura da lesão. Os animais foram submetidos à laparotomia mediana e a indução da peritonite intra-operatória foi obtida através de lesão do cólon previamente distendido pela introdução de uma sonda naso-gástrica via retal e injeção de 2 ml de soro fisiológico. Foram realizados exames bacteriológicos de lavado abdominal obtido por swab esterelizado e exame microscópico de segmento suturado do cólon de amostras obtidas dos grupos A2 e B com 48hs, 96hs e 12 dias de pós-operatório. Todos os animais foram submetidos a necropsia por ocasião do óbito ou no 12º. dia de pós-operatório quando os sobreviventes foram sacrificados. RESULTADOS: Agentes bacterianos semelhantes foram encontrados nos três grupos: E.coli (100%; Enterococcus faecalis (97%; P. mirabilis (90%; Klebsiela pneumoniae (70%; Citobacter freundi (70% e Enterobacter aglomerans (63%. O exame microscópico revelou menor reação inflamatória no grupo esplenectomizado. A causa da morte na maioria foi peritonite nas primeiras 96hs. Houve alto índice de significância de mortalidade entre os animais do Grupo B (80% em relação ao Grupo A2 (sem mortalidade e em relação ao Grupo A1 (35%. CONCLUSÃO: Houve alto índice de significância de mortalidade em vigência de peritonite nos animais esplenectomizados em relação aos animais que não foram esplenectomizados.BACKGROUND: Study the effects of splenectomy on the intra-abdominal infection by bowel flora, consequent to a colonic injury in Wistar rats. METHODS: We used 64 animals, 20 for Group A1 (normal with colon lesion left open, 22 for Group

  2. Resultados de una encuesta sobre tratamiento de la cifosis torácica causada por enfermedad de Scheuermann Resultados de uma pesquisa sobre o tratamento da cifose torácica de Scheuermann Results of a survey on the treatment of thoracic Scheuermann´s kyphosis

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    Claudio Silveri

    2012-06-01

    Full Text Available OBJETIVO: Conocer que parámetros se deben considerar para encarar el tratamiento de la cifosis torácica causada por la enfermedad de Scheuermann. MÉTODO: Se realizó una recolección de informaciones consistente en aplicar un cuestionario a 25 informantes calificados, colegas de diferentes países de Íbero/Latinoamérica miembros de SILACO (Sociedad Íbero/Latinoamericana de Columna y se analizaron los resultados de las mismas. RESULTADOS: Como tratamiento ortopédico, la mayoría se inclinó por el uso del corsé de Milwaukee, indicado en pacientes prepúberes y pospúberes con curvas mayores de 60º y en curvas de menos de 60º si tienen dolor. El tiempo de uso: 22 hrs por día, su mantenimiento hasta llegar a Risser 4 o 5. Se observó una tasa de abandono de 48% al plan terapéutico. En lo referente a tratamiento quirúrgico, las indicaciones surgen: por localización de la deformidad, el valor angular, dolor, edad y estética. La extensión del área a fusionar fue determinada con los espinogramas y radiografías dinámicas. Se eligió la vía posterior como vía de abordaje con barras y tornillos, y en algunos casos la combinación con ganchos. Se evaluaron los resultados del tratamiento quirúrgico (de acuerdo a la opinión del médico, que fueron catalogados como buenos en un 64%. CONCLUSIONES: Se realizó una encuesta de opinión sobre el tratamiento de la cifosis torácica causada por enfermedad de Scheuermann .y se analizaron 25 formularios de respuestas de calificados cirujanos de columna, de diferentes países de Ibero/Latinoamérica miembros de SILACO. La encuesta ha permitido establecer normas de conducta terapéuticas (ortopédicas y quirúrgicas para el tratamiento de esta afección.OBJETIVO: Obter os parâmetros a serem considerados para realizar o tratamento da cifose torácica de Scheuermann. MÉTODOS: Os dados foram obtidos por meio de um questionário que foi respondido por 25 membros da SILACO (Sociedad Ibero

  3. A novel postharvest rot of okra pods caused by Rhizoctonia solani in Brazil Uma nova podridão pós-colheita de frutos de quiabo causada por Rhizoctonia solani

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    Gilmar P. Henz

    2007-06-01

    ngulos quase retos com constrições na base, células da hifa com 6-10 µm de largura com um septo perto da base. Cinco isolados foram obtidos dos frutos infectados e identificados como sendo do grupo de anastomose AG 1-IB. Frutos de quiabo cv. Santa Cruz 47 inoculados com discos de micélio de R. solani com e sem ferimentos e mantidos em câmaras úmidas, a 25 ºC, por sete dias ficaram completamente apodrecidos pelo patógeno, com cor marrom, enquanto somente os frutos com ferimentos mantidos a 12 ºC apresentaram lesões pequenas, variando de 0,6 a 1,0 mm de diâmetro. Em outro experimento, foi demonstrado que o patógeno foi capaz de crescer na superfície de diferentes materiais usados na confecção de embalagens de produtos hortícolas, como madeira de pinus, papelão corrugado, plástico, isopor e folhas de jornal mantidos em câmara úmida (24 ºC, 96 % UR. A ocorrência da doença está relacionada com manuseio pós-colheita inadequado, e a transmissão de propágulos do fungo junto com restos culturais ou partículas de solo. Este é o primeiro relato de R. solani causando podridão pós-colheita em frutos de quiabo no Brasil.

  4. Estudos histomorfométrico e histológico das lesões ósseas causadas por flúor em aves Histomorphometric and histological evaluations of the bone lesions caused by fluoride in chickens

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    Margarida Buss Raffi

    1997-04-01

    Full Text Available Fluoreto de sódio foi administrado na água de 2 grupos de 25 aves, fêmeas, da raça Shaver. O grupo A recebeu flúor do 1 ao 120 dia de idade e o grupo B do 61 ao 120 dia de idade. Cada grupo foi dividido em 5 níveis de tratamento: 0, 25, 50, 200 e 400 ppm de flúor, com 5 animais por tratamento. Todos os animais foram sacrificados aos 120 dias de idade. Para o estudo histomorfométrico foram coletados o fêmur e a tíbia esquerdos, e para o estudo histológico foram coletados fêmur e tíbia direitos. No estudo histomorfométrico do osso compacto observou-se um discreto aumento da porosidade cortical, que não foi estatisticamente significativo. A espessura do osso cortical aumentou nos animais tratados com flúor (pSodium fluoride was administered in the water to 2 groups of 25 Shaver female poultry. Group A received fluoride from 1 to 120 days of age and group B from 61 to 120 days. Each group was divided into 5 treatments, with 5 chickens each, which received 0, 25, 50, 200, and 400 ppm of fluoride in the water, respectively. All animals were killed at 120 days of age. For histomorphometric studies the left femur and tibia were used, and for histologic studies the right femur and tibia. In the cortical bone, cortical porosity was slightly increased by fluoride, but the differences with the control group were not significant. Cortical thickness increased in the animals treated with fluoride (p<0,05, r2 = 0,59 . In the trabecular bone, of group A, trabecular thickness (TT (p<0,05, r2 = 0,63 and trabecular bone volume (TBV (p<0,05, r2 = 0,72 increased in treatments receiving 25-200 ppm, and decreased in the 400 ppm treatment, as it was demonstrated by quadratic regression analysis. In group B there was a positive linear correlation on TT (p<0,05, r2 = 0,98 and TBV (p<0,05, r2 = 0,77 with fluoride ingestion.The osteoid surface was also positively correlated with the amount of fluoride ingested by the animals (p<0,001, r2 = 0,80. These

  5. Freqüência e atividade enzimática de Candida spp. na cavidade oral de pacientes diabéticos do serviço de endocrinologia de um hospital de Fortaleza-CE Frequency and enzymatic activity of Candida spp. oral cavity of diabetic patients of the service of endocrinology of a hospital of Fortaleza-CE

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    Everardo Albuquerque Menezes

    2007-08-01

    Full Text Available O diabetes mellitus (DM, uma doença endócrino-metabólica de alta e crescente prevalência, é citada como responsável pela ocorrência das candidíases orais. A candidíase constitui um espectro de infecções causadas por fungos do gênero Candida, sendo o seu agente mais comum a Candida albicans, embora outras espécies tenham sido identificadas (Candida tropicalis, Candida guillermondii, Candida glabrata, Candida krusei. O objetivo deste trabalho foi avaliar a freqüência e a atividade enzimática de Candida spp. na cavidade oral de pacientes diabéticos atendidos no Serviço de Endocrinologia do Hospital Universitário Walter Cantídio da Universidade Federal do Ceará (HUWC/UFC. Foram coletadas amostras de 48 pacientes diabéticos, de ambos os sexos, com situações variáveis de controle glicêmico. Os materiais clínicos foram colhidos com ajuda de swabs e semeados em placas de Petri contendo ágar-Sabouraud dextrose com cloranfenicol e incubado a 37°C. Os crescimentos foram identificados pelas provas clássicas usadas em micologia. Depois, essas cepas de Candida foram submetidas a provas de detecção de enzimas fosfolipase e proteinase. Destas, 15 amostras (31,25% apresentaram cultura positiva para o gênero Candida. A espécie mais freqüente foi a C. albicans, com 80%, seguida de C. tropicalis (13,3% e C. guillermondii (6,7%. Quanto à pesquisa da atividade enzimática de Candida spp., foi observado que 86,6% delas apresentaram atividade de proteinase e 80%, de fosfolipase. Conclui-se com tais resultados que a C. albicans é a mais freqüente e que as espécies de Candida isoladas possuem fortes atividades enzimáticas.Diabetes mellitus, a endocrine-metabolic disease, of high and increasing prevalence, is cited as responsible by the occurrence of oral candidiasis. Candidiasis constitutes a specter of infections caused by fungi of genera Candida; the most common agent is Candida albicans, but other species have also been

  6. Frecuencia y descripción de lesiones óseas en el tronco y su correlación con las lesiones en tejidos blandos, causadas por proyectil de arma de fuego, en una muestra de necropsias realizadas en el Instituto Nacional de Medicina Legal y Ciencias Forenses, regional Bogotá 2008–2009 / Frequency and description of bony injuries in the trunk and his correlation with the injuries in soft tissues, caused by missile of firearm, in a sample of autopsies realized in the National Institute of Legal Medicine and Forensic Sciences, regional Bogotá 2008-2009

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    Garzón Rodríguez, Néstor Raúl

    2009-01-01

    Este estudio presenta los resultados de la revisión de 306 reportes de necropsias medicolegales practicadas a cadáveres cuyas muertes fueron causadas por proyectil de arma de fuego, con el objetivo de determinar la frecuencia y las características de las lesiones sufridas en el tronco. La muestra correspondía en un 94.5% a cuerpos de genero masculino, con grupos de edad predominante entre 20 y 39 años. El número de disparos vario entre 1 y 14 impactos por cada cuerpo, siendo los mas frecuente...

  7. Candida famata (Candida flareri).

    Science.gov (United States)

    Dmytruk, Kostyantyn V; Sibirny, Andriy A

    2012-11-01

    Candida famata (Candida flareri) belongs to the group of so-called 'flavinogenic yeasts', capable of riboflavin oversynthesis under condition of iron starvation. Some strains of C. famata belong to the most flavinogenic organisms known and were used for industrial production of riboflavin for a long time in the USA. C. famata is characterized by high salt tolerance, growing at NaCl concentrations of up to 2.5  M. Development of basic tools for the metabolic engineering of C. famata, such as a transformation system, selective markers, insertional mutagenesis, a reporter system and others, are described. The developed tools were used for cloning and identification of structural and regulatory genes of riboflavin synthesis. The construction of improved yeast strains producing riboflavin, FMN and FAD, based on the industrial riboflavin-producing strain dep8 and its non-reverting analogue AF4, is also described. PMID:23108915

  8. Candida esophagitis: species distribution and risk factors for infection Esofagite por Candida: distribuição da espécie e fatores de risco para a infecção

    OpenAIRE

    Dimas Alexandre Kliemann; Alessandro Comarú Pasqualotto; Maicon Falavigna; Thiane Giaretta; Luiz Carlos Severo

    2008-01-01

    Although Candida albicans is the main cause of fungal esophagitis, other species such as C. tropicalis, C. krusei and C. stellatoidea have also been implicated. Several studies have identified risk factors for C. albicans esophagitis. However, data for non-C. albicans species is still sparse. The aim of this study was to determine the etiology of Candida esophagitis in our medical centre over an 18-month period. Additionally, we aimed to investigate predisposing conditions for esophageal cand...

  9. Aproveitamento de subproduto industrial de óleos vegetais para produção de riboflavina por Candida guilliermondii DM 644 Utilization industrial waste from vegetal oils riboflavine production by candida guilliermondii DM 644

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    Maria de Lourdes Andrade Pessoa

    2003-12-01

    Full Text Available A produção e consumo de alimentos industrializados têm aumentado a preocupação com suplementação e enriquecimento de alimentos com vitaminas e sais minerais, visando repor as possíveis perdas durante os processos de fabricação, principalmente das vitaminas hidrossolúveis, mais especificamente da vitamina B2 ou riboflavina. Assim sendo, a proposta deste trabalho foi utilizar como componente principal do meio, para produção da riboflavina, um subproduto do refino de óleos vegetais e o microrganismo Candida guillermondii DM 644. A produção da vitamina B2 foi realizada por fermentação em batelada utilizando Erlenmeyer. As condições empregadas foram agitação orbital, ausência de luz, 30°C, e 24h de incubação. A otimização da produção de riboflavina foi realizada através de Delineamento Fatorial Fracionário, para avaliar os efeitos da concentração de matéria graxa, fonte de nitrogênio, pH, velocidade de agitação, fonte de fósforo e extrato de levedura e as possíveis interações. A concentração máxima de riboflavina foi 19,12mg/mL. Os fatores mais importantes para produção de riboflavina foram a concentração de matéria graxa e a fonte de nitrogênio, enquanto que a fonte de fósforo e o extrato de leveduras não estimularam sua biossíntese. A máxima produção foi obtida com matéria graxa a 10g/L, uréia a 2,5g/L e pH 5,0. A velocidade de agitação (200 e 400rpm não interferiu no processo biotecnológico.The ever growing production and consumption of industrialized foods have increased the concern about supplementation and enrichment of food with vitamins and minerals, attempting the replenishment of the possible losses during their production processes, mainly the hydrosoluble vitamins, more specifically B2 vitamin or riboflavin. A subproduct of vegetal oil refining and the microorganism Candida guilliermondii DM 644 were used as the main substrate. The vitamin B2 was produced by the batch

  10. Alteraciones dentales y periodontales causadas por el stripping en ortodoncia.

    OpenAIRE

    Aparicio Merchán, Helena

    2015-01-01

    Desde hace más de setenta años el desgaste interproximal o stripping forma parte de las opciones de las que disponemos en los tratamientos de ortodoncia. Inicialmente fue un procedimiento indicado solo para pacientes adultos principalmente para el tratamiento de las discrepancias dentarias y la prevención de las recidivas mediante la estabilización de los puntos de contacto. Además comenzó a emplearse como alternativa en los casos dudosos de extracción, la eliminación de triángulos n...

  11. Surto de mastite bovina causada por Arcanobacterium pyogenes

    Directory of Open Access Journals (Sweden)

    R.G. Motta

    2011-06-01

    Full Text Available An uncommon outbreak of mastitis caused by Arcanobacterium pyogenes in 26 cows is reported. The epidemiological findings, clinical signs, microbiological exams, somatic cell count, in vitro susceptibility profile of strains, efficacy of intramammary treatment and control measures were discussed. Florfenicol (96.2%, cefoperazona (92.3%, cefaloxin (84.6% and ceftiofur (84.6% were the most effective antimicrobials, and neomicin (27.0% and enrofloxacin (17.4% the least effective antimicrobials.

  12. Atividade antifúngica de óleos essenciais sobre espécies de Candida Antifungal activity from essential oils on Candida species

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    Igara de Oliveira Lima

    2006-06-01

    Full Text Available A candidíase apresenta-se como uma infecção fúngica superficial ou profunda causada por leveduras pertencentes ao gênero Candida, sendo considerada a principal infecção micótica em ambiente nosocomial. O objetivo do presente estudo foi determinar a concentração inibitória mínima - CIM do óleo essencial de Cinnamomum zeylanicum Blume, Citrus limon Risso, Eucalyptus citriodora HK, Eugenia uniflora L., Peumus boldus Benth e de Rosmarinus officinialis L. sobre cepas de Candida albicans, C. guilliermondii, C. krusei, C. parapsilosis, C. stellatoidea e C. tropicalis. Os ensaios de atividade antifúngica foram realizados através da técnica de difusão em meio sólido. Os óleos essenciais de C. zeylanicum e P. boldus mostraram os mais destacáveis resultados, visto que inibiram o crescimento de 58% das cepas ensaiadas e apresentaram CIM de 4%.Candidiasis occurs as a superficial or profound fungal infection caused by yeasts belonging to the Candida genus. This infectious has been considered the main mycotic infection in the nosocomial environment. The aim of this study was to determine the minimum inhibitory concentration - MIC of Cinnamomum zeylanicum Blume, Citrus limon Risso, Eucalyptus citriodora HK, Eugenia uniflora L., Peumus boldus Benth and Rosmarinus officinialis L. essential oils against Candida albicans, C. guilliermondii, C. krusei, C. parapsilosis, C. stellatoidea e C. tropicalis strains. The antifungal activity assays were carried out by solid medium diffusion technique. C. zeylanicum and P. boldus essential oils showed the most prominent results which inhibited the growth of 58% of the assayed yeasts strains and presented MIC of 4%.

  13. Effect of topical 0.5% povidone-iodine compared to 5% natamycin in fungal keratitis caused by Fusarium solani in a rabbit model: a pilot study Efeito tópico de iodo-povidona 0,5% comparado com natamicina 5% em ceratites fúngicas causadas por Fusarium solani em um modelo animal de coelhos: estudo piloto

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    Lauro Augusto de Oliveira

    2008-12-01

    tratados por 3 dias e sacrificados em seguida. As córneas foram removidas cirurgicamente, maceradas e incubadas em meio BHI. Semeou-se culturas em placas de ágar Sabouraud, diariamente, durante 7 dias, e contou-se o número de unidades formadoras de colônias (UFC. Os coelhos foram avaliados clinicamente durante o período de tratamento. RESULTADOS: Os grupos iodo-povidona e natamicina demonstraram melhor eficácia do que o grupo controle considerando-se o número de coelhos nos quais não houve crescimento de colônias. Entretanto, não houve diferença estatística significante entre os 3 grupos quando se analizou o número de UFC (p>0,05. CONCLUSÃO: Este estudo demonstrou considerações metodológicas importantes na utilização de modelos animais para o teste de agentes antifúngicos. Usando a metodologia de contar UFC e com este tamanho amostral, administração tópica de iodo-povidona 0,5% não demonstrou benefício do tratamento de ceratite fúngica experimental causada por Fusarium solani quando comparado com a administração tópica de natamicina 5%.

  14. Surtos epidêmicos de dermatite causada por mariposas do gênero Hylesia (Lepidóptera: Hemileucidae no Estado de São Paulo, Brasil Epidemic outbreaks of dermatitis caused by Hylesia (Lepidoptera: Hemileucidae, in S. Paulo State, Brazil

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    Carmen Moreno Glasser

    1993-06-01

    Full Text Available Surtos epidêmicos de dermatite causada por Hylesia sp foram assinalados no litoral do Estado de São Paulo, no período de dezembro de 1989 a dezembro de 1991. O fenômeno assumiu sua maior intensidade em Bertioga, Município de Santos, onde foram registrados 612 casos. Foram atingidos outros 12 municípios da região, estimando-se que algumas centenas de casos procuraram os serviços de saúde locais. Na maioria dos casos verificou-se lesões eritemato-pápulo-pruriginosas, que regrediram em média de 7 a 14 dias. Para tratamento foram utilizados anti-histamímicos sistêmicos corticosteróides tópicos e compressas frias. Ocorreram três episódios epidêmicos no período citado, todos eles coincidindo com o início da estação chuvosa (novembro a janeiro. A cada episódio verificou-se um deslocamento do fenômeno no sentido Norte-Sul. A principal medida profilática utilizada foi a divulgação, junto à população, das medidas de redução de exposição ao agente. Para prédios com elevada infestação por mariposas, foi estudada a efetividade da aplicação de inseticida residual, como medida de redução dos níveis de infestação por mariposas. Obteve-se resultados satisfatórios com deltametrina na dose de 50 mg/m² de parede.Epidemic outbreaks of dermatitis caused by Hylesia sp wich occurred in the coastal region of the State of S. Paulo during the period of December 1989 to December 1991 are confirmed. The incident assumed its greatest intensity in Bertioga, in Santos County, where 612 cases were registered. The outbreak also affected 12 other counties in that region and it was estimated that hundred of affected persons sought the Local Health Service Care. The majority presented with erythematous and prutiginous lesions and papula which lasted 7 to 14 days on average. Treatment consisted of systemic administration of antihistamines, and the use of topic corticosteroides and cold compresses. During the abovementioned period, three

  15. Avaliação de Passifloraceas, fungicidas e Trichoderma para o manejo da Podridão-do-colo do maracujazeiro, causada por Nectria haematococca Evaluation of Passifloraceas, fungicides and Trichoderma for passion fruit collar rot handling, caused by Nectria haematococca

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    Ivan Herman Fischer

    2010-09-01

    Full Text Available A baixa produtividade do maracujazeiro-amarelo é devida, muitas vezes, a problemas fitossanitários, sendo a Podridão-do-colo, causada por Nectria haematococca, um dos principais problemas na maioria dos Estados produtores do Brasil. O controle desta doença é basicamente preventivo, evitando a introdução do agente patogênico na área. Sendo assim, o presente trabalho teve como objetivos: a avaliar o comportamento do maracujazeiro-amarelo 'Afruvec', 'IAC 275' e 'Maguary', do tipo pé-franco, e 'Afruvec' enxertado em cinco espécies de Passiflora (P. alata, P. maliformis, P. morifolia, P. mucronata e P. suberosa, em pomar com histórico de Podridão-do-colo; e b avaliar a eficiência de produtos químicos (oxicloreto de cobre, procloraz e tiabendazol e biológicos (Trichoderma harzianum e Trichoderma sp. no controle da Podridão-do-colo em maracujazeiro-amarelo 'Afruvec', sob condições de campo. As espécies P. maliformis, P. suberosa e P. alata, empregadas como porta-enxerto, apresentaram maior resistência à Podridão-do-colo do maracujazeiro em relação às plantas não enxertadas. Os produtos químicos e biológicos, aplicados em intervalos mensais ou quinzenais no colo da planta (500 mL de calda, não foram eficientes no controle da doença.The low productivity of yellow passion fruit is due often to the phytosanitary problems, being the Collar rot caused by Nectria haematococca, one of the main problems in the majority of the producing States of Brazil. The disease control is basically preventive, avoiding the introduction of the pathogen in the area. So, the present work aimed at: a evaluating the behavior of ungrafted yellow passion fruit cultivars 'Afruvec', 'IAC 275' and 'Maguary' and 'Afruvec' passion fruit grafted on five Passiflora species (Passiflora alata, P. maliformis, P. morifolia, P. mucronata and P. suberosa in orchard where the disease is frequent; and b evaluating the efficiency of chemical (copper oxychloride

  16. AVALIAÇÃO DA AÇÃO DE EXTRATOS VEGETAIS SOBRE A FORMAÇÃO DE BIOFILMES POR Candida albicans

    Directory of Open Access Journals (Sweden)

    Noara Taline Pereira dos Reis

    2011-01-01

    Full Text Available The biofilm consists of a diverse community of microorganisms attached on biotic and abiotic surfaces, formed by Candida albicans. In the oral cavity, the presence of biofilm is the first step towards the development of dental caries and periodontal disease, which makes it necessary to assess the effectiveness of new alternatives, such as the use of plant extracts, to promote prevention. We evaluated the inhibitory activities of the extract of the jabuticabeira leaf (Myrciaria cauliflora and leaf extract of guava (Psidium guajava L in biofilm formation by Candida albicans, as this yeast is generally associated with diseases of the mouth. Microplates were used for yeast cultivation and to assess the degree of biofilm formation inhibition through the tack checked by turbidity in the ELISA reader. None of the extracts inhibited biofilm formation at the concentrations used.

  17. Reação de híbridos, linhagens e progênies de pimentão à requeima causada por Phytophthora capsici e ao mosaico amarelo causado por Pepper yellow mosaic vírus (PepYMV Reaction of hybrids, lines and progenies of sweet pepper the blight caused by Phytophthora capsici and to Pepper yellow mosaic virus (PepYMV

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    Ildon Rodrigues do Nascimento

    2007-02-01

    Full Text Available A requeima do pimentão (Capsicum annuum L. causada por Phytophthora capsici e o mosaico amarelo causado por PepYMV são prioridades nos programas de melhoramento de pimentão em andamento no Brasil. Foram avaliados três híbridos comerciais (Magali R, Atenas F1 e Fortuna Super F1, cinco linhagens, 20 híbridos experimentais e duas progênies F2:4 do programa de melhoramento da Universidade Federal de Lavras/HortiAgro Sementes quanto à reação a P. capsici e a PepYMV. Os experimentos foram montados independentemente em blocos casualizados, com quatro e duas repetições, respectivamente. Cada parcela foi constituída de oito plantas instaladas em bandejas de 128 células, que foram mantidas em estufa com cobertura plástica e laterais teladas. As avaliações foram feitas do 4º ao 14º e do 15º ao 40º dias após a inoculação (DAI para P. capsici e PepYMV, respectivamente. A inoculação com P. capsici foi feita pormeio da distribuição de 5 mL de suspensão com 10(4 zoósporos/mL no solo ao redor do colo de cada planta. A inoculação mecânica de PepYMV foi feita a partir de macerados de folhas de Nicotiana tabacum cv. TNN, previamente infectadas. Os híbridos Magali R e Fortuna Super, foram suscetíveis a P. capsici, enquanto que o acesso Criollo de Morellos 334, a linhagem PIM-013 e as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 (ambas originadas do cruzamento com Criollo de Morellos, foram resistentes. Reação de resistência a P. capsici foi também observada para os híbridos experimentais que tiveram PIM-013 como uma das linhagens parentais. Criollo de Morellos 334, as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3, o híbrido comercial Magali R e outros 6 híbridos experimentais que tinham como um dos genitores a linhagem MYR-29 forma ressitentes ao PepYMV. As progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 são fontes promissoras de resistência a ambos os patógenos para serem desenvolvidas e exploradas em programas de melhoramento

  18. Terapia de resgate com amiodarona em crianças com grave disfunção ventricular esquerda causada por veneno de escorpião Terapia de rescate con amiodarona en niños con severa disfunción ventricular izquierda ocasionada por veneno de escorpión Antiadrenergic rescue therapy with amiodarone in children with severe left ventricular dysfunction secondary to scorpion envenomation

    Directory of Open Access Journals (Sweden)

    Justo J. Santiago

    2010-01-01

    Full Text Available FUNDAMENTO: As crianças picadas por escorpião, pressintam ativação maciça do sistema nervoso simpática com vários graus de disfunção sistólica ventricular esquerda. OBJETIVO: Testar um protocolo de resgate em crianças com grave disfunção ventricular esquerda causada por picada de escorpião. Métodos: Quatro crianças após serem picadas por escorpião foram submetidas a: Encubação endotraqueal e suporte respiratório, eletrocardiograma, radiografia de tórax, ecocardiograma e determinação sérica da norepinefrina e troponina I. As análises foram repetidas após 12, 24 e 48 horas. As seguintes medicações intravenosas foram administradas: dobutamina 4-6 μg/kg/min; amiodarona 3 mg/kg durante duas horas, com dose de manutenção de 5 mg/kg/dia; e furosemida 0,5 mg/kg. Amiodarona, dobutamina e furosemida foram administradas durante as primeiras 48 horas. Bloqueadores beta-adrenérgicos e inibidores da enzima conversora da angiotensina foram administrados até 48 após a internação, uma vez que o estado clínico havia melhorado e a fração de ejeção ventricular esquerda encontrava-se acima de 0,35%. RESULTADOS: Na admissão, a dosagem da norepinefrina foi 1.727,50± 794,96 pg/ml, a de troponina I 24,53 ± 14,09 ng/ml e a fração de ejeção do ventrículo esquerdo foi 0,20 ± 0,056. Após 12 horas, os níveis séricos de norepinefrina e de troponina I diminuíram para a metade dos valores iniciais e a fração de ejeção aumentou para 0,32 ± 0,059. Durante as 24 e 48 horas subseqüentes, a fração de ejeção elevou-se para 0,46 ± 0,045 (pFUNDAMENTO: Los niños con picaduras de escorpión sufren activación masiva del sistema nervioso simpático con varios grados de disfunción sistólica ventricular izquierda. OBJETIVO: Probar un protocolo de rescate en niños con disfunción ventricular severa izquierda ocasionada por picadura de escorpión. MÉTODOS: Cuatro niños tras un escorpión picarlas se sometieron a: incubaci

  19. Candida albicans isoladas da cavidade bucal de crianças com síndrome de Down: ocorrência e inibição do crescimento por Streptomyces sp Candida albicans isolated from buccal cavity of children with Down's syndrome: occurrence and growth inhibition by Streptomyces sp

    Directory of Open Access Journals (Sweden)

    José Daniel Gonçalves Vieira

    2005-10-01

    Full Text Available Comparação entre a presença de leveduras de Candida na cavidade bucal de crianças sem e com síndrome de Down mostrou-se estatisticamente significante no caso de crianças afetadas por esta cromossomopatia, tornando-as mais predispostas à candidíase bucal, provavelmente favorecida pelas alterações anátomo-fisiológicas da boca em decorrência da trissomia do cromossomo 21. Recidivas constantes de candidíase bucal em crianças portadoras desta alteração cromossômica levou a busca de prováveis alternativas terapêuticas. Visando determinar a atividade antifúngica de Streptomyces sp isolados de diferentes solos brasileiros, 5 cepas foram testadas frente a Candida albicans, oriundas da cavidade bucal de crianças com síndrome de Down. Observou-se que os isolados apresentaram uma diversidade de tamanho dos halos (9-31mm de diâmetro de inibição de crescimento das leveduras, sugerindo uma possível utilização em terapêutica antifúngica.Comparison of the presence of Candida yeasts in the buccal cavity of children without and with Down's syndrome showed a statistically significant difference in the case of children that were affected by this chromosomopathy, rendering them more predisposed to buccal candidiasis, probably due to anatomicophysiologic alterations of the mouth resulting from trisomy of chromosome 21. Constant recurrence of buccal candidiasis in children with this chromosomal alteration lead to the search for a possible therapeutic alternative. Seeking to determine the antifungal activity of Streptomyces sp isolated from various Brazilian soils, 5 strains have been tested for Candida albicans isolated from the buccal cavity of children with Down's syndrome. It was observed that the isolate presented a diversity in the size of the halos (9-31mm in diameter of growth inhibition of the yeasts, suggesting a possible use as a therapeutic antifungal.

  20. Human cutaneous leishmaniasis caused by Leishmania (Viannia braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas

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    C.A. Cuba Cuba

    1996-12-01

    pacientes humanos com lesões cutâneas de leishmaniose na Província de Santiago del Estero, no Norte da Argentina. Os procedimentos de diagnóstico foram: biópsias de lesões para utilização em esfregaços e inoculação em hamster; aspiração (com agulha de úlceras, para cultura "in vitro". As técnicas imunodiagnósticas empregadas foram a IFAT-IgG e o teste intradérmico de Montenegro. Oito cepas de parasitas foram isoladas, sendo estas obtidas de pacientes com lesões ativas. Todas as cepas foram inicialmente caracterizadas biologicamente por seu comportamento na infecção experimental do hamster, mensuração dos amastigotas e promastigotas e crescimento "in vitro". As mesmas oito cepas foram logo identificadas e caracterizadas a nível de espécie, devido a sua reatividade frente a um painel de anticorpos monoclonais subgênero e espécie-específicos. Isso foi realizado utilizando o teste de Imunofluorescência Indireta (IFAT/MAbs e de um procedimento de Dot-ELISA. Nós concluímos a partir da análise de serodema dos isolados argentinos que: MHOM/AR/92/SE-1; SE-2; SE-4; SE-8; SE-8-I; SE-30; SE-34 e SE-36 são Leishmania (Viannia braziliensis. Entretanto, três dos isolados de Leishmania (SE-1; SE-2 e SE-30 não foram reconhecidos quando testados com um anticorpo monoclonal de reconhecida alta espécie-especificidade (clone B-18, marcador consagrado de Leishmania (Viannia braziliensis, revelando a existência de dois tipos de serodemas entre as cepas estudadas. Cinco dos oito extratos solúveis de Leishmania foram submetidos à eletroforese em gel de amido de camada fina e subseqüentemente examinadas a fim de constatar a atividade das enzimas MPI, MDH, 6PGD, NH, NH-D, SOD, GPI e ES. Fundamentados nos estudos dos corridos eletroforéticos obtidos nos ensaios isoenzimáticos chegamos à conclusão que as cepas MHOM/AR/92 SE-1; SE-2; SE-4; SE-8 e SE-8-I são Leishmania (Viannia braziliensis. É necessário analisar mais enzimas antes de enquadrá-los nos

  1. Avaliação das perdas causadas por vírus na produção da batata I: Vírus do enrolamento da fôlha Evaluation of yield losses induced by potato leaf roll

    Directory of Open Access Journals (Sweden)

    F. P. Cupertino

    1970-01-01

    Full Text Available A comparação entre as produções de 50 pares de plantas sadias e afetadas, de nove variedades de batata, mostra que a redução na produção causada pela infecção secundária do vírus do enrolamento da fôlha foi, em média, de 60,8% no pêso total dos tubérculos produzidos e de 75,5% no pêso dos tubérculos do tipo graúdo ("especial" mais "primeira". As perdas na produção total variaram de 44,6% (variedade Patrones a 73,4% (variedade Aquila, e na de tubérculos graúdos, de 49,8% (Delta A a 86,0% (Gunda.A comparison of 50 pairs of field healthy and leaf roll infected potato plants of nine varieties indicated that the total yield reduction was on the average 60.7 per cent. Yield reduction of the two largest and most marketable potato sizes ("especial" and "primeira" reached 75.6 per cent. Aquila and Gunda had the highest yield losses (73.4 and 72.0 per cent; Delta A and Patrones had the lowest (47.6 and 44.6 per cent. Reduction in the yield of the larger tuber sizes was generally greater than total losses.

  2. Virulence factors of non-Candida albicans Candida species

    OpenAIRE

    Silva, Sónia Carina; Negri, M.; Monteiro, D. R.; Henriques, Mariana; Oliveira, Rosário; Azeredo, Joana

    2012-01-01

    Infections caused by Candida species (candidosis) have greatly increased over recent years, mainly due to the escalation of the AIDS epidemic, population ageing, increasing number of immunocompromised patients and the more widespread use of indwelling medical devices. Besides Candida albicans, non-Candida albicans Candida (NCAC) species such as Candida glabrata, Candida tropicalis and Candida parapsilosis are now frequently identified as potential human pathogens. Candida species pathogenicit...

  3. Bilateral polymicrobial osteomyelitis with Candida tropicalis and Candida krusei

    DEFF Research Database (Denmark)

    Kaldau, Niels Christian; Brorson, Stig; Jensen, Poul Einar;

    2012-01-01

    We present a case of bilateral polymicrobial osteomyelitis with Candida tropicalis and Candida krusei, and review the literature on Candida osteomyelitis.......We present a case of bilateral polymicrobial osteomyelitis with Candida tropicalis and Candida krusei, and review the literature on Candida osteomyelitis....

  4. Características epidemiológicas de las nuevas infecciones causadas por el VIH comparadas con los casos de sida: La epidemia de VIH/ sida en el País Vasco Epidemiological characteristics of new HIV infections compared with AIDS cases: The HIV/AIDS epidemic in the Basque Country [Spain

    OpenAIRE

    Daniel Zulaika; Koldo Agirrebengoa; Ander Andía; Julio Arrizabalaga; José María Bustillo; Mari Mar Cámara; Jesús Corral; Mari Carmen Orive; Julio Goikoetxea; José Antonio Iribarren; Josefina López de Munain; José Manuel Lorenzo; María José Martín Gudino; Eduardo Martínez; José Mayo

    2004-01-01

    Objetivo: Describir las características epidemiológicas de los nuevos diagnósticos de infección por el VIH en el período 1997-2001 y comparlas con los casos de sida (1991-2001). Métodos: Se han recogido retrospectivamente los datos de las nuevas infecciones por el VIH ocurridas en el País Vasco (1997-2001) y se han comparado con los casos de sida (1991-2001). Resultados: Se han diagnosticado 912 nuevas infecciones por el VIH. El diagnóstico de VIH coincidió con el de sida en 299 (32,8%) de la...

  5. Candida Albicans

    OpenAIRE

    Dr. Maria Magdalena Simatupang

    2009-01-01

    義歯性口内炎患者のデンチャープラーク中には、多数の真菌が認められることから、これら真菌が衰症の原因菌の一つとされている。このようなデンチャープラーク中の真菌には、Candida属が高頻度に検出され、中でもCandida albicansの検出率が著しく高いことが知られている。本真菌は、酵母(Y)型並びにフィラメント(F)型の二つの形態をとる二形性真菌であり、種々の因子によりその形態が変化することが、古くから知られている。しかし、その詳細な機構については未だ不明な点が多い。著者は、C.albicansが培地中のビオテン濃度により形態変化を受ける事実に着目し、本菌の二形性と脂質代謝との間に、なんらかの関連性があるのではないかとの作業仮設のもとに、以下の実験を行った。 本研究は、Candida albicans A IFO 1385株を用いて行った。使用培地は、サブローグルコース培地(2% グルコース、1% ペプトン、 0.5% イーストエキス)(medium A)並びにメチオニン含有合成培地(medium B)である。培養温度は、それぞれY型薗並びにF型菌を得るために、25℃...

  6. Características epidemiológicas de las nuevas infecciones causadas por el VIH comparadas con los casos de sida: La epidemia de VIH/ sida en el País Vasco Epidemiological characteristics of new HIV infections compared with AIDS cases: The HIV/AIDS epidemic in the Basque Country [Spain

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    Daniel Zulaika

    2004-04-01

    Full Text Available Objetivo: Describir las características epidemiológicas de los nuevos diagnósticos de infección por el VIH en el período 1997-2001 y comparlas con los casos de sida (1991-2001. Métodos: Se han recogido retrospectivamente los datos de las nuevas infecciones por el VIH ocurridas en el País Vasco (1997-2001 y se han comparado con los casos de sida (1991-2001. Resultados: Se han diagnosticado 912 nuevas infecciones por el VIH. El diagnóstico de VIH coincidió con el de sida en 299 (32,8% de las nuevas infecciones. Las relaciones heterosexuales han sido el mecanismo de transmisión más frecuente, seguido de la transmisión por vía parenteral y las relaciones homosexuales y bisexuales, con diferencias significativas (pObjective: To describe the epidemiological characteristics of new cases of HIV infection diagnosed from 1997-2001 and compare them with AIDS cases (1991-2001. Methods: Data were retrospectively collected on new cases of HIV infection detectad in the Basque Country (1997-2001 and were compared with AIDS cases (1991-2001. Results: A total of 912 new cases of HIV infection were diagnosed. In 299 of the new cases (32.8%, HIV and AIDS were diagnosed simultaneously. The most common mechanism of transmission was heterosexual transmission, followed by intravenous and homo/bisexual transmission. Significant epidemiological differences (p<0.001 were found with regard to AIDS cases. Conclusions: Sexual transmission has replaced intravenous drug use as the most common mechanism of HIV transmission. A large percentage of patients were simultaneously diagnosed with HIV and AIDS, indicating the need for new prevention strategies.

  7. Diagnosis of neonatal group B Streptococcus sepsis by nested-PCR of residual urine samples Diagnóstico de sepse neonatal causada pelo estreptococo do grupo B por meio de dupla amplificação de amostras residuais de urina

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    Bruno Nicolino Cezarino

    2008-03-01

    four patients. Moreover, PCR has enabled us to use residue volumes of urine samples collected by non invasive, non sterile methods, what is technically adequate as GBS is not part of the normal urine flora, thus avoiding invasive procedures such as suprapubic bladder punction or transurethral catheterization. At the same time, the use of urine instead of blood samples could help preventing newborns blood spoliation.O estreptococo do grupo B (GBS constitui a causa mais freqüente de sepse neonatal precoce. O teste de referência continua sendo o isolamento em cultura, apesar de apresentar problemas de sensibilidade. O objetivo do presente estudo foi validar uma técnica de dupla amplificação e determinar a possibilidade do uso de amostras residuais de urina colhidas por método não invasivo, não estéril, para a confirmação da sepse por GBS em recém-nascidos. As amostras foram amplificadas com primers do principal gene de superfície do GBS. A insuficiência de volume de material biológico para a realização de exames para suporte de vida, além de outros necessários à identificação do agente etiológico de infecções é muito freqüente em recém-nascidos. Mesmo assim, decidimos definir critérios bastante rigorosos para a inclusão de pacientes na casuística: os recém-nascidos deveriam apresentar sinais e sintomas compatíveis com infecção pelo GBS; deveriam ter tido ao menos uma amostra enviada para cultura, podendo ser sangue, urina ou líquor; disponibilidade de volumes residuais dessas amostras, ou de outras colhidas no dia da hospitalização, antes da introdução da antibioticoterapia, de forma a possibilitar a análise por PCR, e evolução favorável com a antibioticoterapia empírica. Em apenas um dos quatro recém-nascidos a infecção foi confirmada por cultura, enquanto nos outros três casos a infecção foi considerada presuntiva (pacientes preencheram os critérios de inclusão, mas o GBS não foi isolado. De um total de 12 amostras

  8. Reducción de la concentración de ion potasio en las vinazas de destilación de alcohol por medio de la propagación de Candida utilis

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    Miguel A. Otero-Rambla

    2012-01-01

    Full Text Available Las levaduras son capaces de acumular cantidades apreciables de la mayoría de los minerales presentes en su medio de crecimiento. Una buena proporción de los contenidos de cenizas (7,5-8,1 % encontrados en la levadura panadera o en la recuperada de la fabricación de cerveza, está constituida por fosfato de potasio. Adicionalmente, la levadura posee la habilidad de acumular, en elevadas concentraciones, otros iones presentes en los sustratos en los que crecen, aun cuando no les sean necesarios. Las vinazasde destilación de alcohol contienen cerca de 70 % del potasio, proveniente de los fertilizantes químicos empleados en los campos de caña; así, la fertilización y el riego con estas aguas residuales debe ser calculada cuidadosamente para evitar la salinización del suelo. Cuando la levadura Candida utilis crece en un medio compuesto por vinazas de destilación, sales nutrientes (fosfato y sulfato de amonio y un estimulador de crecimiento microbiano, muestra una gran resistencia a la concentración de potasio en las condiciones de cultivo continuo. En los estudios experimentales realizados, las células de levadura fueron propagadas bajo las condiciones mencionadas en el párrafo anterior, en presencia de concentraciones crecientes de K2O en el medio de propagación, desde 2,5 gl-1 ( correspondientes a vinazas procedentes de la fermentación de melazas de caña hasta 25 gl-1. La tasa máxima de crecimiento (μmax varió desde 0,32 a 0,28 h-1 para los valores extremos señalados, en tanto que el rendimiento biomasa-sustrato se encontró en el rango de 0,23 a 0,18. Estos resultados sugieren que la levadura propagada sobre vinazas de destilación pueden reducir significativamente el contenido de potasio de estos residuales, haciéndolos más adecuados para propósitos de irrigación. De acuerdo con los informes de evaluación sobre la utilización de esta levadura en la alimentación animal, no se encontraron efectos negativos sobre la salud

  9. Influência do fotoperíodo e da temperatura na intensidade de doença causada por Fusarium graminearum em Egeria densa e E. najas Influence of the photoperiod and temperature on the intensity of disease caused by Fusarium graminearum in Egeria densa and E. najas

    OpenAIRE

    C.R. Borges Neto; C.Q. Gorgati; R.A. Pitelli

    2005-01-01

    Um isolado de Fusarium graminearum vem sendo estudado na UNESP, campus de Jaboticabal, como agente de controle biológico de Egeria densa e de E. najas, plantas aquáticas submersas que causam problemas em reservatórios de hidrelétricas. O presente trabalho teve por objetivo estudar os efeitos do fotoperíodo e da temperatura no controle dessas plantas em condições de laboratório. A cada dois dias foram avaliados os sintomas nas plantas inoculadas com F. graminearum, atribuindo-se notas de sever...

  10. Avaliação de produtos químicos comerciais, in vitro e in vivo, no controle da doença foliar, mancha branca do milho, causada por Pantoea ananatis Evaluation of commercial chemical products, in vitro and in vivo in the control of foliar disease, maize white spot, caused by Pantoea ananais

    OpenAIRE

    Cleide Aparecida Bomfeti; Walter Fernandes Meirelles; Edneia Aparecida Souza-Paccola; Carlos Roberto Casela; Alexandre da Silva Ferreira; Ivanildo Evódio Marriel; Luzia Doretto Paccola-Meirelles

    2007-01-01

    Uma bactéria identificada como Pantoea ananatis foi recentemente isolada de lesões jovens da doença mancha branca do milho de plantas naturalmente infectadas. Esta bateria reproduziu sintomas semelhantes aos da doença quando inoculada em plantas de milho em casa de vegetação. Estudos anteriores realizados por outros autores demonstraram que o controle desta doença em condições de campo foi obtido pelo uso de fungicidas, principalmente o Mancozeb, nas fases iniciais de seu desenvolvimento. O o...

  11. Immunohistochemical changes in kidney glomerular and tubular proteins caused by rattlesnake (Crotalus vegrandis venom Cambios inmunohistoquímicos en proteínas de túbulo y glomérulo renal causadas por el veneno de la serpiente de cascabel (Crotalus vegrandis

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    María E. Girón

    2003-10-01

    Full Text Available Renal damage is an important cause of death in patients who have survived the early effects of severe crotalid envenomation. Extracellular matrix of renal tissue is altered by Crotalus toxin activities. The aim of this study was to describe how cytoskeletal proteins and basal membrane components undergo substantial alterations under the action of Crotalus vegrandis crude venom and its hemorrhagic fraction (Uracoina-1 in mice. To detect the proteins in question, the immunoperoxidase method with monoclonal and polyclonal antibodies was used. Cell types within renal lesions were characterized by phenotypic identification, by means of immunohistologic analysis of marker proteins using different primary antibodies against mesangial cells, endothelial cells, cytoskeletal proteins (intermediate filament, extracellular matrix and basal membranes. Samples for morphological study by standard procedures (biotin-streptavidin-peroxidase technique using light microscopy were processed. Positive and negative controls for each antigen tested in the staining assay were included. After crude venom and hemorrhagic fraction inoculation of mice, the disappearance of cytoskeletal vimentin and desmin and collagen proteins in the kidney was observed. In extracellular matrix and basal membranes, collagen type IV from envenomed animals tends to disappear from 24 h to 120 h after venom injection.El daño renal es una causa importante de muerte en pacientes que sobreviven a los efectos iniciales de los severos envenenamientos crotálicos. El objetivo de este estudio ha sido el describir como las proteínas del citoesqueleto y los componentes de membrana basal muestran alteraciones importantes en su manifestación, bajo la acción del veneno crudo de Crotalus vegrandis y una fracción hemorrágica (Uracoina-1 del mismo veneno ya que, la matriz extracelular del tejido renal es alterada por la actividad de estas toxinas. Para detectar las proteínas en cuestión se utiliz

  12. The histopathology of cutaneous leishmaniasis due to Leishmania (Leishmania mexicana in the Yucatan peninsula, Mexico Histopatologia de la leishmaniasis cutánea causada por Leishmania (Leishmania mexicana en la península de Yucatán, México

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    Fernando J. Andrade-Narvaez

    2005-08-01

    Full Text Available Localized Cutaneous Leishmaniasis (LCL known as "chiclero's ulcer" in southeast Mexico, was described by SEIDELIN in 1912. Since then the sylvatic region of the Yucatan peninsula has been documented as an endemic focus of LCL. This study of 73 biopsies from parasitological confirmed lesions of LCL cases of Leishmania (Leishmania mexicana infection was undertaken: 1 to examine host response at tissue level; and 2 to relate manifestations of this response to some characteristics of clinical presentation. Based on Magalhães' classification we found that the most common pattern in our LCL cases caused by L. (L. mexicana was predominantly characterized by the presence of unorganized granuloma without necrosis, (43.8%. Another important finding to be highlighted is the fact that in 50/73 (68.5% parasite identification was positive. There was direct relation between the size of the lesion and time of evolution (r s = 0.3079, p = 0.03, and inverse correlation between size of the lesion and abundance of amastigotes (r s = -0.2467, p = 0.03. In view of the complexity of clinical and histopathological findings, cell-mediated immune response of the disease related to clinical and histopathological features, as so genetic background should be studied.La Leishmaniosis Cutánea Localizada (LCL mejor conocida como "úlcera del chiclero" en el sureste de México fue descrita por SEIDELIN en 1912. Desde entonces la región selvática de la península de Yucatán ha sido identificada como un área endémica de LCL. En el presente estudio se analizaron 73 biopsias de lesiones de casos de LCL causados por Leishmania (Leishmania mexicana con el fin de: 1 examinar la respuesta a nivel tisular; y 2 relacionar las manifestaciones de esta respuesta con ciertas características de la presentación clínica. Con base en la clasificación histopatológica de Magalhães el patrón histopatológico más frecuente se caracterizó por la presencia de granuloma desorganizado y

  13. Influência do fotoperíodo e da temperatura na intensidade de doença causada por Fusarium graminearum em Egeria densa e E. najas Influence of the photoperiod and temperature on the intensity of disease caused by Fusarium graminearum in Egeria densa and E. najas

    Directory of Open Access Journals (Sweden)

    C.R. Borges Neto

    2005-09-01

    Full Text Available Um isolado de Fusarium graminearum vem sendo estudado na UNESP, campus de Jaboticabal, como agente de controle biológico de Egeria densa e de E. najas, plantas aquáticas submersas que causam problemas em reservatórios de hidrelétricas. O presente trabalho teve por objetivo estudar os efeitos do fotoperíodo e da temperatura no controle dessas plantas em condições de laboratório. A cada dois dias foram avaliados os sintomas nas plantas inoculadas com F. graminearum, atribuindo-se notas de severidade da doença, por um período de oito dias após a inoculação. Também foi avaliado o crescimento das plantas por meio do ganho de massa fresca, expresso em porcentagem. A maior severidade da doença foi observada quando ambas as espécies foram mantidas no escuro, e a menor, em fotoperíodo de 12 horas. A temperatura de 30 ºC proporcionou maior severidade de doença em ambas as espécies. A espécie E. densa apresentou maior produção de massa fresca no regime de 12 horas de luz e de temperaturas abaixo de 25 ºC e menor produção no regime de escuro total e nas temperaturas de 30 e 35 ºC. Por sua vez, E. najas apresentou menor produção de massa fresca no regime de escuro e nas temperaturas de 25 a 35 ºC.A promising Fusarium graminearum isolate has been evaluated as a potential biocontrol agent of two important aquatic weeds, Egeria densa and E. najas. This work aimed to study the effects of photoperiod and temperature on the control of these plants under laboratory conditions. The symptons in the plants inoculated with F. graminearum were evaluated every two days, with disease severity being evaluated through a grade scale and plant growth by fresh weight gain, expressed in percentage. The highest severity grades were observed whin both species were kept in the dark and the lowest under 12 hours photoperiod. The temperature of 30 ºC provided the higest disease severity for both species. The species E. densa presented the highest fresh

  14. Fotossensibilização primária em eqüídeos e ruminantes no semi-árido causada por Froelichia humboldtiana (Amaranthaceae Primary photosensitization in equidae and ruminants in the Brazilian semi-arid caused by Froelichia humboldtiana (Amaranthaceae

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    Luciano A. Pimentel

    2007-01-01

    Full Text Available Fotossensibilização é freqüente em eqüinos no semi-árido da região Nordeste, mas jumentos, mulas, ovinos e bovinos são, também, afetados. A dermatite afeta, principalmente, áreas de pele despigmentadas e os animais se recuperam após serem retirados das pastagens. Para comprovar a etiologia da enfermidade Froelichia humboldtiana (Roem. et Schult. Seub., coletada no campo foi administrada no mesmo dia da colheita ou após ser mantida em refrigerador por 1-4 dias, por períodos de 30 ou mais dias, ad libitum como único alimento, a 2 jumentos e um ovino branco e, como único alimento volumoso, a um eqüino branco. Esses animais não manifestaram sinais clínicos e as atividades séricas de gama-glutamiltransferase (GGT, aspartato-aminotransferase (AST e alanino-aminotransferase (ALT ficaram dentro dos valores normais. Em outro experimento, um ovino foi colocado a pastar diariamente, durante o dia, preso por uma corda em uma área que tinha exclusivamente F. humboldtiana, por um período de 26 dias. Lesões características de fotossensibilização foram observadas 4-5 dias após o início do experimento. Após cessar o consumo da planta, no 26º dia, o ovino recuperou-se totalmente em 30 dias. Em outro experimento, 4 ovinos foram também colocados, presos por cordas, na mesma área. Outros 4 permaneceram como controles em uma pastagem vizinha, mas sem F. humboldtiana. Lesões de pele, características de fotossensibilização foram observadas após 11-25 dias de consumo de F. humboldtiana. As atividades séricas de AST e GGT, e os níveis de bilirrubina sérica permaneceram dentro dos valores normais. No final do período de permanência em pastagens de F. humboldtiana, 2 ovinos foram abatidos e 2 foram retirados da pastagem. Os que foram abatidos não apresentaram lesões macroscópicas nem histológicas do fígado; os outros dois se recuperaram das lesões da pele 17 e 20 dias após o fim do pastejo. Uma égua e seu potro foram

  15. Identificação de enfermidades agudas causadas por animais e plantas em ambientes rurais e litorâneos: auxílio à prática dermatológica Identification of acute diseases caused by animals and plants in wild environments: contribution to dermatologic practice

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    Vidal Haddad Junior

    2009-08-01

    Full Text Available FUNDAMENTOS: Nos últimos anos, existe um aumento progressivo do contato de moradores urbanos com ambientes selvagens devido a atividades de lazer. Com isso, algumas dermatites pouco conhecidas podem ser observadas nas clínicas privadas e ambulatórios dermatológicos, especialmente nos inícios de semana e finais de férias. OBJETIVOS: Obter e fornecer informações para dermatologistas sobre o problema. PACIENTES E MÉTODOS: O autor observou, em adultos e crianças, dermatites agudas associadas a plantas ou animais em Ubatuba, cidade litorânea de São Paulo, por dois meses (junho/julho de 2006 e na Faculdade de Medicina de Botucatu, também por dois meses (junho/julho de 2007. RESULTADOS: Foram observados 25 pacientes na área rural e 43 na área litorânea nas condições estabelecidas. Em áreas rurais, foram mais observadas fitofotodermatites e picadas de insetos, enquanto em áreas litorâneas traumas por ouriços-do-mar e fitofotodermatites predominaram; entretanto, em ambas as áreas ocorreram outros acidentes de difícil identificação na prática diária. CONCLUSÕES: Devemos estar atentos ao fato de o paciente procurar o dermatologista somente após as fases agudas dos acidentes. Informações sobre as enfermidades mais comuns e suas características podem ser muito úteis para a prática nos consultórios. O autor sugere uma tabela algorítmica para auxílio diagnóstico.BACKGROUND: In recent years, there has been increasing contact between human beings that live in urban regions and the wild environment due to a series of activities. As a result, some poorly known dermatitis may present in private and dermatological clinics, especially early in the week and at the end of vacation periods. OBJECTIVES: To obtain and provide information for dermatologists on the problem. PATIENTS AND METHODS: The author observed adult and pediatric patients with acute dermatitis associated with plants or animals in Ubatuba, coastal city of Sao Paulo

  16. Influência do fotoperíodo e da temperatura na intensidade de doença causada por Fusarium graminearum em Egeria densa e E. najas Effects of photoperiod and temperature in the development of disease caused Fusarium graminearum on Egeria densa and E. najas

    Directory of Open Access Journals (Sweden)

    Carlos R. Borges Neto

    2004-06-01

    Full Text Available Um isolado de Fusarium graminearum vem sendo estudado na UNESP, Campus de Jaboticabal, como agente de controle biológico de Egeria densa e E. najas, macrófitas aquáticas submersas, muito problemáticas em reservatórios de hidrelétricas. O presente trabalho teve por objetivo estudar os efeitos do fotoperíodo (0; 4; 8 e 12 h diárias de luz e da temperatura (15 ºC, 20 ºC, 25 ºC, 30 ºC e 35 ºC no controle destas plantas em condições de laboratório. A cada dois dias foram avaliados os sintomas nas plantas inoculadas com F. graminearum, atribuindo-se notas de severidade de doença, por um período de oito dias após a inoculação. Também foi avaliado o crescimento das plantas por meio do ganho de massa fresca, expresso em porcentagem. A maior severidade de doença foi observada quando ambas as espécies foram mantidas no escuro e a menor em fotoperíodo de 12 h. A temperatura de 30 ºC proporcionou maior severidade de doença em ambas espécies. Egeria densa apresentou maior produção de massa fresca no regime de 12 h de luz e de temperaturas abaixo de 25 ºC e menor produção no regime de escuro total e nas temperaturas de 30 ºC e 35 ºC. Egeria najas apresentou menor produção de massa fresca no regime de escuro total e nas temperaturas de 25 ºC a 35 ºC.A very promising Fusarium graminearum isolate was evaluated as a potential biocontrol agent for two important submerged aquatic weeds, Egeria densa and E. najas. In previous studies, the fungus showed high control of the species and satisfactory specificity. However, under some conditions, the results were unstable, probably due to abiotic factors affecting the pathogenesis. In order to evaluate the influences of the photoperiod (0; 4; 8 and 12 daily hours of light and temperature (15 ºC, 20 ºC, 25 ºC, 30 ºC and 35 ºC, studies were carried-out under laboratory conditions in completely randomized plots. The photoperiod effects were evaluated under 25 ºC and pH 7,0. The

  17. Thrush and Other Candida Infections

    Science.gov (United States)

    ... Text Size Email Print Share Thrush and Other Candida Infections Page Content Article Body The fungus Candida is ... thrush, frequently occurs in infants and toddlers. If Candida infections become chronic or occur in the mouth of ...

  18. Omeprazole and misoprostol for preventing gastric mucosa effects caused by indomethacin and celecoxib in rats Omeprazol e misoprostol na prevenção de lesões de mucosa gástrica causadas por indometacina e celecoxib em ratos

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    Míriam Elias Cavallini

    2006-06-01

    ástrica com omeprazol e misoprostol. MÉTODOS: A amostragem consistiu 150 ratos machos da raça Wistar, com peso médio de 200g, divididos em quatro grupos, a saber: grupo A, subdividido em grupos A1 e A2 - pré-tratamento com omeprazol (20 mg/rato durante sete dias, e no oitavo dia receberam o AINEs, sendo A1 (20 ratos receberam celecoxib (1mg/rato e A2 (20 ratos receberam indometacina (12,5mg/rato. O grupo B, subdividido em grupo B1 e B2 - pré-tratamento com misoprostol (20ìg/rato durante sete dias e no oitavo dia receberam AINEs, sendo B1 (20 ratos receberam celecoxib (1mg/rato e B2 (20 ratos receberam indometacina (12,5mg/rato. O grupo C não recebeu citoproteção durante sete dias e no oitavo dia recebeu AINEs, sendo C1 (20 ratos receberam celecoxib (1mg/rato , C2 (20ratos receberam indometacina (12,5mg/rato, C3 (20 ratos receberam celecoxib e grupo D - grupo controle, no qual dez ratos foram observados recebendo ração e água ad libitum. A seguir, no 9º dia (de todos os grupos, os estômagos eram removidos e avaliados macro e microscopicamente para a identificação das lesões gástricas. RESULTADOS: Na análise macroscópica, os grupos A2, B2 e C2 apresentaram número de lesões por cm²/animal significativamente elevados, sendo respectivamente 18,55 lesões por cm²/animal, 16,25 lesões por cm²/animal e 13,55 lesões por cm²/animal. Na análise microscópica, a porcentagem da mucosa com lesão mostrou diferença significativa entre os grupos A1, B1, C1 quando comparados com os grupos A2, B2 e C2 (p<0,0001. Os resultados da média da extensão/lesão e da média da profundidade das lesões não mostraram diferenças estatísticas significativas entre os grupos A2, B2 e C2. A média do edema mostrou diferença estatística significativa entre os grupos A2 e D; B2 e C2 e entre C2 e D (p<0,05. CONCLUSÕES: A indometacina na concentração empregada provoca número significativo de lesões macro e microscópicas na mucosa gástrica de ratos quando comparadas ao

  19. Síndrome do ápice orbitário causada por herpes zóster oftálmico: relato de caso e revisão da literatura Herpes zoster ophthalmicus and orbital apex syndrome: case report and literature review

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    Kenzo Hokazono

    2009-10-01

    Full Text Available OHerpes Zoster Oftálmico (HZO decorre da infecção pelo vírus da varicela-zoster que permanece latente no gânglio de Gasser até que seja reativado e comprometa a divisão oftálmica do nervo trigêmeo. HZO freqüentemente causa manifestações oftalmológicas como lesões vesiculares palpebrais, ceratoconjuntivite, esclerite, uveíte, paralisia oculomotora, miosite orbitária e neurite óptica. Raramente o acometimento do ápice da órbita pode ser a manifestação inicial desta grave afecção. Este trabalho relata um caso de síndrome do ápice orbitário associado à meningite, causado por HZO e que foi tratado com corticosteróide e aciclovir sistêmicos.Herpes Zoster ophthalmicus (HZO is caused by a varicella-zoster virus infection which remains latent in the ganglion of Gasser until it is reactivated and compromise the ophthalmic division of the trigeminal nerve. HZO commonly causes neuro-ophthalmic complications such as vesicular lesions in the eyelids, keratoconjunctivitis, sclertis, uveitis, ocular palsy, orbital miositis and optic neuritis. HZO rarely presents as an orbital apex syndrome. This paper describes a patient with of orbital apex syndrome associate and meningitis caused by HZO which was treated with systemic steroids and acyclovir.

  20. Glycemic acute changes in type 2 diabetics caused by low and high glycemic index diets Las variaciones de la glucosa aguda en individuos con diabetes tipo 2 causada por las dietas de bajo y alto índice glucémico

    Directory of Open Access Journals (Sweden)

    C. E. Gonçalves Reis

    2011-06-01

    Full Text Available Introduction: Low-glycemic index diets may improve the glycemic control in type 2 diabetes but the debate over their effectiveness continues. Objectives: To test the effects of low-glycemic index diets on acute glycemic control (2 days by measuring capillary blood glucose in patients with type 2 diabetes. Methods: This was a crossover randomized clinical trial with 12 type 2 diabetics which were randomly divided into 2 groups and targeted the following draft diets for low and high glycemic index (LGI and HGI for 2 consecutive days in 2 consecutive weeks. Group 1 followed an LGI diet in week 1 and an HGI diet in week 2, group 2 adopted the contrary. They were oriented to maintain medication and lifestyle and to follow the recommendations. Measurements were made of glycemia capillaries in 2 days (fasting, before lunch, post-prandial lunch and before dinner and one last in fasting on day 3. A food record during the days and the counting of carbohydrates meals was made. The software SigmaStat (version 2.03 was used, with a statistical significance criterion of p Introducción: Dietas de bajo índice glucémico pueden mejorar el control glucémico en la diabetes tipo 2, pero sigue el debate sobre su eficacia. Objetivos: Evaluar los efectos de las dietas bajas en el índice glucémico en el control glucémico agudo (2 días por la medición de glucosa en sangre capilar en pacientes con diabetes tipo 2. Métodos: Se realizó un ensayo clínico cruzado y aleatorizado con 12 pacientes diabéticos tipo 2. Fueron divididos en 2 grupos: dietas de bajo y alto índice glucémico (BIG y AIG. Las dietas fueron consumidas por 2 días consecutivos, en 2 semanas distintas. Para el grupo 1 fue administrado la dieta BIG en la semana 1 seguida de la dieta AIG en la semana 2. En contrario se dio para el grupo 2. Se recomendó a los pacientes que mantuviesen la medicación y el estilo de vida estables. Se midió y registro la glucemia capilar en 2 días (en ayunas

  1. Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae transmitted virus Mancha clorótica do Clerodendrum, uma enfermidade causada por um vírus do tipo nuclear, transmitido pelo ácaro Brevipalpus phoenicis (Acari:Tenuipalpidae

    Directory of Open Access Journals (Sweden)

    Elliot Watanabe Kitajima

    2008-02-01

    por ácaros Brevipalpus (VTB. Brevipalpus phoenicis coletados de C. x speciosum sintomático e transferidos para plantas sadias de C. x speciosum reproduziram as lesões. O ácaro também transmitiu o patógeno para C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi e Tetragonia expansa, as quais exibiram manchas cloróticas e/ou necróticas. O vírus também foi transmitido mecanicamente para Chenopodium amaranticolor, C. quinoa, G. globosa, H. cannabinus, H. coccineus e T. expansa, além de C. x speciosum. Plantas de C. amaranticolor e C. quinoa mantidas a 28 - 30ºC desenvolveram infecção sistêmica. Em todos os tecidos sintomáticos das plantas-teste inoculadas, examinados ao microscópio eletrônico, foram encontrados efeitos citopáticos do tipo nuclear causado por VTB. O vírus foi purificado a partir de folhas com infecção sistêmica de C. amaranticolor e C. quinoa. Injeções de preparações purificadas em coelho geraram um anti-soro policlonal que reagiu especificamente com o antígeno homólogo em teste de ELISA. As evidências obtidas indicam que as manchas cloróticas do Clerodendrum estão associadas a um VTB do tipo nuclear, tentativamente denominado de vírus da mancha clorótica do Clerodendrum (Clerodendrum chlorotic spot virus- ClCSV.

  2. Reaction of arracacha genotypes to the root soft rot caused by Pectobacterium chrysanthemi Reação de genótipos de mandioquinha-salsa à podridão-mole das raízes causada por Pectobacterium chrysanthemi

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    Gilmar Paulo Henz

    2005-01-01

    Full Text Available The purpose of this paper was to screen thirty-two arracacha genotypes for their reaction to root soft rot. Twenty roots of each genotype were inoculated with two Pectobacterium chrysanthemi isolates in a randomized experiment (10 roots/isolate. After inoculation, roots were individually wrapped with PVC film and kept at 26ºC in closed plastic bags. Soft rot lesions were recorded after 36 hours and genotypes were grouped in four classes of susceptibility by cluster analysis: 10 were less susceptible, 16 intermediate, 3 susceptible and 3 very susceptible. All the tested arracacha genotypes showed only variation in the degree of susceptibility.O objetivo deste trabalho foi avaliar a reação de 32 genótipos de mandioquinha-salsa à podridão-mole das raízes. Vinte raízes de cada genótipo foram inoculadas com dois isolados de Pectobacterium chrysanthemi em um experimento casualizado (10 raízes/isolado. Após a inoculação, as raízes foram embaladas com filmes de PVC e mantidas a 26ºC em sacos de plástico. As lesões de podridão-mole foram avaliadas após 36 horas e os genótipos agrupados em quatro classes de suscetibilidade por análise de agrupamento: 10 foram menos suscetíveis, 16 intermediários, 3 suscetíveis e 3 muito suscetíveis. Todos os genótipos avaliados demonstraram apenas variação no grau de suscetibilidade.

  3. Insuficiência respiratória aguda causada por pneumonia em organização secundária à terapia antineoplásica para linfoma não Hodgkin Acute respiratory failure caused by organizing pneumonia secondary to antineoplastic therapy for non-Hodgkin's lymphoma

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    Adriell Ramalho Santana

    2012-12-01

    Full Text Available Doenças difusas do parênquima pulmonar pertencem a um grupo de doenças de evolução geralmente subaguda ou crônica, mas que podem determinar insuficiência respiratória aguda. Paciente masculino, 37 anos, em terapia para linfoma não Hodgkin, admitido com tosse seca, febre, dispneia e insuficiência respiratória aguda hipoxêmica. Iniciadas ventilação mecânica e antibioticoterapia, porém houve evolução desfavorável. Tomografia computadorizada de tórax mostrava opacidades pulmonares em "vidro fosco" bilaterais. Devido ao paciente ter feito uso de três drogas relacionadas à pneumonia em organização (ciclofosfamida, doxorrubicina e rituximabe e quadros clínico e radiológico serem sugestivos, iniciou-se pulsoterapia com metilprednisolona com boa resposta. Pneumonia em organização pode ser idiopática ou associada a colagenoses, drogas e neoplasias, e geralmente responde bem a corticoterapia. O diagnóstico é anatomopatológico, mas condições clínicas do paciente não permitiam a realização de biópsia pulmonar. Pneumonia em organização deve ser diagnóstico diferencial em pacientes com aparente pneumonia de evolução desfavorável ao tratamento antimicrobiano.Interstitial lung diseases belong to a group of diseases that typically exhibit a subacute or chronic progression but that may cause acute respiratory failure. The male patient, who was 37 years of age and undergoing therapy for non-Hodgkin's lymphoma, was admitted with cough, fever, dyspnea and acute hypoxemic respiratory failure. Mechanical ventilation and antibiotic therapy were initiated but were associated with unfavorable progression. Thoracic computed tomography showed bilateral pulmonary "ground glass" opacities. Methylprednisolone pulse therapy was initiated with satisfactory response because the patient had used three drugs related to organizing pneumonia (cyclophosphamide, doxorubicin and rituximab, and the clinical and radiological symptoms were

  4. Avaliação de produtos químicos comerciais, in vitro e in vivo, no controle da doença foliar, mancha branca do milho, causada por Pantoea ananatis Evaluation of commercial chemical products, in vitro and in vivo in the control of foliar disease, maize white spot, caused by Pantoea ananais

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    Cleide Aparecida Bomfeti

    2007-03-01

    Full Text Available Uma bactéria identificada como Pantoea ananatis foi recentemente isolada de lesões jovens da doença mancha branca do milho de plantas naturalmente infectadas. Esta bateria reproduziu sintomas semelhantes aos da doença quando inoculada em plantas de milho em casa de vegetação. Estudos anteriores realizados por outros autores demonstraram que o controle desta doença em condições de campo foi obtido pelo uso de fungicidas, principalmente o Mancozeb, nas fases iniciais de seu desenvolvimento. O objetivo deste estudo foi avaliar a freqüência de isolamento da bactéria P. ananatis a partir de plantas infectadas coletadas na região de Londrina, Estado do Paraná, e reproduzir sintomas da doença através de inoculações artificiais em plantas de milho em casa de vegetação. Utilizando os produtos químicos testados anteriormente por outros autores para o controle desta doença a campo, foi também objetivo deste trabalho avaliar o potencial destes produtos na inibição da bactéria tanto em condições de laboratório como em condições de infecção natural. Os resultados mostraram que P. ananatis foi isolada em 40% das lesões jovens coletadas a campo e quando inoculada em casa de vegetação sob condições controladas reproduziu sintomas semelhantes aos observados a campo. Entre os produtos químicos testados, o fungicida Mancozeb mostrou-se eficiente no controle da doença a campo, em concordância com os relatos anteriores. Este produto inibiu completamente o crescimento da bactéria em laboratório, explicando os resultados obtidos a campo. Os demais produtos não foram eficientes no controle a campo e eles também não inibiram a bactéria em laboratório. Estes resultados representam evidências adicionais de que a bactéria P. ananatis é o agente causal da doença mancha branca do milho.A bacterium identified as Pantoea ananatis was recently isolated from young lesions of the disease maize white spot from infected plants in

  5. Onychomycosis by Scytalidium dimidiatum: report of two cases in Santa Catarina, Brazil Onicomicose por Scytalidium dimidiatum: relato de dois casos em Santa Catarina, Brasil

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    Lucíola Nascimento Pontarelli

    2005-12-01

    Full Text Available Onychomycoses constitute pathologies frequently seen in dermatological practice worldwide. Usually, they are caused by two groups of pathogenic fungi: dermatophytes and yeasts of the Candida genus. However, in a small fraction of the cases, the etiologic agents comprise nondermatophyte molds, belonging to several genera and species. The objective of this study was to present two cases of onychomycosis associated to the mold Scytalidium dimidiatum in patients residing in two cities of Santa Catarina State, Brazil. Aspects of fungal pathogenesis, as well as the epidemiological characteristics and laboratory diagnosis, are discussed.Onicomicoses se apresentam como infecções fúngicas localizadas, muito freqüentes na prática dermatológica. Na grande maioria das vezes, são causadas por dois grupos: dermatófitos e leveduras do gênero Candida. Entretanto, em um pequeno percentual dos casos, os agentes etiológicos compreendem fungos filamentosos não-dermatófitos, pertencentes a vários gêneros e espécies. O objetivo deste trabalho foi o de apresentar dois casos de onicomicose associados à espécie Scytalidium dimidiatum em pacientes residentes em dois municípios do estado de Santa Catarina, Brasil. São discutidos aspectos relacionados a sua patogênese, epidemiologia, diagnóstico laboratorial e tratamento.

  6. Relationships of systemic IgG antibody response and lesions caused by Oestrus ovis L. larvae (Diptera: Oestridae in infected goats - Interacciones de la respuesta sistémica de anticuerpos IgG y las lesiones causadas por larvas de Oestrus ovis L. (Diptera: Oestridae en cabras infectadas

    Directory of Open Access Journals (Sweden)

    Angulo-Valadez, Carlos E

    2009-11-01

    la glándula salival para elserodiagnóstico de la estrosis caprina usando la prueba de ELISA. Secapturaron larvas de tercer estadio (L3, se disectaron para removerla glándula salival y obtener la fuente de antígenos (CGS. Seexaminaron en necropsias 250 cabras. El peso, sexo y edad fueronregistrados. Los senos frontales y cavidades cornuales se examinaronpara la presencia de O. ovis. La intensidad de las lesiones en cabrasinfectadas (n=38 se registraron de acuerdo con una tabla deseveridad. Los sueros (n=125 se analizaron por ELISA para detectarla respuesta humoral de IgG. La prevalencia anual de la estrosiscaprina fue 73.9%. Una asociación baja positiva (r=0.38, P<0.05 seobservó entre la carga larvaria y la severidad de las lesionesencontradas. En general, se observaron alta sensibilidad (90.82% ybaja especificidad (25.93% en la prueba de ELISA. Comoconclusiones, los daños patológicos mayores se asociaron al númeroy presencia de larvas tempranas L2 y L3, probablemente inducidospor la muda larvaria. Los antígenos CGS demostraron ser valiosos enla prueba de ELISA para el diagnóstico de la estrosis caprina.

  7. Insights into Candida world : the extracellular milieu

    OpenAIRE

    Martins, Margarida

    2010-01-01

    Over the last years fungi have emerged as a major cause of human disease. Candida albicans is the most common cause of opportunistic mycoses, albeit Non-Candida albicans Candida (NCAC) species, namely Candida dubliniensis, Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis, are emerging as pathogens. Candida species factors that might influence the pathogenesis of infection include the ability to: undergo a reversible conversion between yeast and fil...

  8. Intestinal intussusception and occlusion caused by small bowel polyps in the Peutz-Jeghers syndrome. Management by combined intraoperative enteroscopy and resection through minimal enterostomy: case report Intussuscepção intestinal e oclusão intestinal causada por pólipos em intestino delgado na síndrome Peutz-Jeghers. Tratamento com ressecção por enterotomia associado a enteroscopia intra-operatória: relato de caso

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    Joaquim J. Gama-Rodrigues

    2000-12-01

    Full Text Available The Peutz-Jeghers syndrome is a hereditary disease that requires frequent endoscopic and surgical intervention, leading to secondary complications such as short bowel syndrome. CASE REPORT: This paper reports on a 15-year-old male patient with a family history of the disease, who underwent surgery for treatment of an intestinal occlusion due to a small intestine intussusception. DISCUSSION: An intra-operative fiberscopic procedure was included for the detection and treatment of numerous polyps distributed along the small intestine. Enterotomy was performed to treat only the larger polyps, therefore limiting the intestinal resection to smaller segments. The postoperative follow-up was uneventful. CONCLUSION: We point out the importance of conservative treatment for patients with this syndrome, especially those who will undergo repeated surgical interventions because of clinical manifestation while they are still young.A síndrome de Peutz-Jeghers é uma doença de caráter hereditário que freqüentemente requer intervenções endoscópicas e cirúrgicas repetidas, levando a complicações secundárias como, por exemplo, a síndrome do intestino curto. RELATO DE CASO: Relatamos neste artigo o caso de paciente de 15 anos, masculino, com história familiar para a doença, que foi submetido ao procedimento cirúrgico para tratar oclusão devido a intussucepção de intestino delgado. DISCUSSÃO: Associou-se método fibroscópico intra-operatório com a finalidade de detecção e ressecção de numerosos pólipos distribuídos em todo o intestino delgado. Realizaram-se enterotomias para a retirada dos pólipos maiores, restringindo-se, portanto, a ressecção intestinal a segmentos menores. A evolução clínica foi boa. CONCLUSÃO: Ressalta-se a importância do manejo de forma conservadora dos pacientes portadores desta síndrome, principalmente naqueles cujas manifestações clínicas de importância cirúrgica aparecem precocemente.

  9. Podridão de frutos de melancia causada por Sclerotium rolfsii no estado de Alagoas

    OpenAIRE

    QUEIROZ FÁTIMA M.; MUNIZ M. F. S.; MENEZES MARIA; BARROS PERICLES G.

    2002-01-01

    A new fruit rot disease caused by Sclerotium rolfsii on watermelon (Citrullus lanatus) was observed in the State of Alagoas. The pathogenicity of the fungus was confirmed by artificial inoculations.This is the first report of S. rolfsii causing fruit rot in watermelon in Northeast of Brazil.

  10. Enoftalmia causada por uso tópico unilateral de Bimatoprost

    OpenAIRE

    Guilherme Herzog Neto

    2016-01-01

    RESUMO O autor descreve o caso de uma paciente que apresenta o olho direito com o sulco palpebral profundo e enoftalmia deste lado, tratado durante uma década com Bimatoprost tópica. Concluem que os achados clínicos são provavelmente efeitos colaterais do medicamento.

  11. Antibioticoterapia intravenosa domiciliaria en el tratamiento de las infecciones causadas por microorganismos multirresistentes

    OpenAIRE

    Oltean, Dan-Marian

    2012-01-01

    L'objectiu d'aquest treball és descriure els tres primers anys d'experiència en teràpia ambulatoria domiciliària endovenosa (TADE) en infeccions per microorganismes multiresistents a la nostra Unitat d'Hospitalització a Domicili alhora que avaluar la seva seguretat i eficacia. El grup control són la resta dels pacients amb TADE ingressats a la Unitat. En el grup estudi hi ha hagut més tornades inesperades i reingressos, probablement en relació a la major comorbilitat i dependència fisica dels...

  12. Caída de la flor en kiwi causada por bacterias fitopatógenas

    OpenAIRE

    González, A.J.

    2010-01-01

    La cáida de flor en kiwi causa pérdidas importantes de producción cuando las condiciones climatológicas son favorables al desarrollo de las bacterias. En Asturias se han descrito dos especies bacterianas, "Pseudomonas viridiflava" y "P. syringae", como agentes causales de este problema

  13. Control cultural de la podredumbre radical causada por Phytophthora cinnamomi en dehesas de encina

    OpenAIRE

    Serrano Moral, Socorro

    2012-01-01

    En este trabajo se ha estudiado el efecto in vitro de varios productos de Ca2+ y K+ (CaO, CaCO3, CaCl2, Ca(NO3)2, CaSO4, KOH, KNO3, KCl, KIO3 y K2SO4) sobre el crecimiento micelial, producción de esporangios y clamidosporas (esporas de resistencia) y germinación de esporangios (producción de zoosporas infectivas) de P. cinnamomi. Aunque ninguno de los productos testados a pH~6 inhibió el crecimiento micelial del patógeno, el CaO, CaCO3, CaSO4, KOH y KIO3 inhibieron de forma eficaz...

  14. Oftalmomiíase externa causada por Dermatobia hominis Dermatobia hominis provoking external ophthalmomyiasis

    OpenAIRE

    Abelardo de Souza Couto Junior; Jociana Paludo; Fernanda de Souza Santana; Márcio Neves Leão; Maria de Fátima Pinheiro Gonçalves

    2010-01-01

    Relato de caso de oftalmomiíase externa em uma paciente de 82 anos, diabética e hipertensa, residente na zona rural, que inicialmente apresentava um quadro sugestivo de celulite pré-septal e hordéolo, com dor, edema, eritema e nodulação localizada na pálpebra superior e bulbo ocular normal. Foi então tratada com antibiótico e corticóide local, que levou a redução do processo inflamatório, possibilitando a realização de adequado exame físico, com a eversão da pálpebra superior, sendo estabelec...

  15. Candida Infection of the Bloodstream - Candidemia

    Science.gov (United States)

    Candida Infection of the Bloodstream– Candidemia Fungal Disease Series #4 Candida is the single most important cause of ... Where in my body can I get a Candida infection? Candida infection can happen in almost any part ...

  16. Genetics of Candida albicans.

    OpenAIRE

    Scherer, S.; Magee, P T

    1990-01-01

    Candida albicans is among the most common fungal pathogens. Infections caused by C. albicans and other Candida species can be life threatening in individuals with impaired immune function. Genetic analysis of C. albicans pathogenesis is complicated by the diploid nature of the species and the absence of a known sexual cycle. Through a combination of parasexual techniques and molecular approaches, an effective genetic system has been developed. The close relationship of C. albicans to the more...

  17. Anti-Candida activity of Quercus infectoria gall extracts against Candida species

    OpenAIRE

    Nur Saeida Baharuddin; Hasmah Abdullah; Wan Nor Amilah Wan Abdul Wahab

    2015-01-01

    Background: Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species. Objective: This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species. Materials and Methods: Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida t...

  18. Candida infection of the skin

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/000880.htm Candida infection of the skin To use the sharing features ... of the warm, moist conditions inside the diaper. Candida infection is particularly common in people with diabetes and ...

  19. Compactação causada pelo manejo de plantas invasoras em latossolo vermelho-amarelo cultivado com cafeeiros

    Directory of Open Access Journals (Sweden)

    Paula Sant'Anna Moreira Pais

    2011-12-01

    Full Text Available O manejo de plantas invasoras é considerado uma das principais atividades que promovem degradação da estrutura do solo em lavouras cafeeiras, devido à compactação do solo causada pelas operações de controle de plantas invasoras. O objetivo deste estudo foi determinar o modelo de capacidade de suporte de carga para o manejo de plantas invasoras sem capina, bem como, utilizando esse modelo, qual manejo de plantas invasoras causa menor ou maior compactação do solo. Este estudo foi realizado em um Latossolo Vermelho-Amarelo (LVA cultivado com cafeeiros da cutivar Topázio MG 1190 desde 2006, localizado na Fazenda Experimental da EPAMIG, na comunidade Farias, em Lavras-MG. Os manejos de plantas invasoras avaliados foram: na linha de tráfego da entrelinha - grade de discos, herbicida de pós-emergência, herbicida de pré-emergência, roçadora e trincha; e no centro da entrelinha, onde não houve tráfego - amendoim forrageiro (Arachis pintoi, braquiária (Brachiaria decumbens, capina manual, crotalária (Crotalaria juncea e soja (Glicine max L. A amostragem consistiu de duas etapas: uma para determinar o modelo de capacidade de suporte de carga para o manejo de plantas invasoras sem capina; e outra para avaliar a compactação promovida pelos outros manejos de plantas invasoras. A fim de determinar o modelo de capacidade de suporte de carga para o manejo sem capina, foram coletadas no centro da entrelinha 20 amostras com estrutura indeformada nas profundidades de 0-3, 10-13 e 25-28 cm, totalizando 60 amostras. Essas amostras foram submetidas ao ensaio de compressão uniaxial para obter as pressões de pré-consolidação e as umidades volumétricas, que foram usadas para determinar o modelo de capacidade de suporte de carga. Para determinar a compactação causada pelos manejos de plantas invasoras, realizados por meio do controle mecânico, foram coletadas em janeiro de 2010, nas linhas de tráfego das entrelinhas, 180 amostras com

  20. Performance of Candida ID, a New Chromogenic Medium for Presumptive Identification of Candida Species, in Comparison to CHROMagar Candida

    OpenAIRE

    Willinger, Birgit; Hillowoth, Cornelia; Selitsch, Brigitte; Manafi, Mammad

    2001-01-01

    Candida ID agar allows identification of Candida albicans and differentiation of other Candida species. In comparison with CHROMagar Candida, we evaluated the performance of this medium directly from 596 clinical specimens. In particular, detection of C. albicans after 24 h of incubation was easier on Candida ID (sensitivity, 96.8%) than on CHROMagar (sensitivity, 49.6%).

  1. Choque séptico por ehrliquiosis

    OpenAIRE

    Viviana Hernández-de Mezerville; Juan I Padilla-Cuadra

    2007-01-01

    La ehrliquiosis humana es una infección zoonótica transmitida por "garrapatas duras" causada por Anaplasma sp y Ehrlichia sp. Puede presentarse como un cuadro pseudogripal con fiebre, malestar general, mialgias y cefalea o hasta un choque séptico con falla multiorgánica y muerte. La mitad de los pacientes requieren atención hospitalaria en algún momento y se reporta una mortalidad de hasta el 5% secundaria a enfermedades oportunistas o a hemorragias. El diagnóstico se puede sospechar visualiz...

  2. Reclassification of Candida guilliermondii FTI 20037 as Candida tropicalis based on molecular phylogenetic analysis Reclassificação de Candida guilliermondii FTI 20037 como Candida tropicalis baseada na análise filogenética molecular

    Directory of Open Access Journals (Sweden)

    Luanne Helena Augusto Lima

    2003-11-01

    Full Text Available Yeasts of the genus Candida are of clinical importance and also have many industrial applications, mainly in the food industry. The yeast Candida guilliermondii FTI 20037 has been extensively studied in order to establish a biotechnological process for the production of xylitol. The goal of this study was to verify the taxonomic classification of this strain based on the analysis of rDNA sequences and the xyl1 gene. DNA fragments from these sequences were amplified by PCR and BLAST analysis revealed strong identity with the corresponding sequences from Candida tropicalis. Based on these results, we propose that C. guilliermondii FTI 20037 must be reclassified as C. tropicalis.As leveduras do gênero Candida possuem tanto importância clínica como diversas aplicações industriais, principalmente na indústria de alimentos. A levedura Candida guilliermondii FTI 20037 tem sido exaustivamente estudada pois pretende-se utilizá-la no estabelecimento de um processo biotecnológico para a produção de xilitol. O objetivo deste trabalho foi verificar a classificação taxonômica desta levedura por análise de sequências do rDNA e do gene xyl1. Fragmentos correspondentes a estas regiões foram amplificados por PCR e a análise destas sequências por BLAST revelou alta identidade com sequências correspondentes de Candida tropicalis. Estes resultados nos levam a propor que C. guilliermondii FTI 20037 deva ser reclassificada como C. tropicalis.

  3. La responsabilidad del Estado por el hecho del legislador

    OpenAIRE

    María Consuelo Alonso; Eric Leiva Ramírez

    2011-01-01

    Analizar el fenómeno de la responsabilidad del Estado causada por los hechos del legislador ha sido un trabajo desarrollado tanto por la doctrina como por la jurisprudencia internacional a mediados del siglo xx. En Francia, por ejemplo, se presentó el primer antecedentejurisprudencial en el año de 1934. En España, su estudio empezó a gestionarse con la promulgación de la Constitución de 1978, dándose el primer fallo judicial en el año de 1993. Para el caso colombiano, la Constitución de 1991 ...

  4. Candida's arranged marriage.

    Science.gov (United States)

    Gow, N A; Brown, A J; Odds, F C

    2000-07-14

    Biologists who study the fungus Candida albicans have always assumed that this organism reproduces asexually because they have not found evidence of mating, meiosis, or a haploid stage of the life cycle. However, as Gow et al. explain in a Perspective, sequencing of the C. albicans genome has revealed the existence of a possible mating type locus. This finding has now been extended to demonstrate actual mating in the fungus (Hull et al., Magee and Magee). PMID:10917850

  5. Determinação das propriedades catalíticas em meio aquoso e orgânico da lipase de Candida rugosa imobilizada em celulignina quimicamente modificada por carbonildiimidazol Assessment of catalytic properties in aqueous and organic media of lipase from Candida rugosa immobilized on wood cellulignin activated with carbonyldiimidazole

    Directory of Open Access Journals (Sweden)

    Fabrício M. Gomes

    2006-07-01

    Full Text Available Microbial lipase from Candida rugosa was immobilized by covalent binding on wood cellulignin (Eucaliptus grandis chemically modified with carbonyldiimidazole. The immobilized system was fully evaluated in aqueous (olive oil hydrolysis and organic (ester synthesis media. A comparative study between free and immobilized lipase was carried out in terms of pH, temperature and thermal stability. A higher pH value (8.0 was found optimal for the immobilized lipase. The optimal reaction temperature shifted from 37 °C for the free lipase to 45 °C for the immobilized lipase. The pattern of heat stability indicated that the immobilization process tends to stabilize the enzyme. Kinetics tests at 37 °C following the hydrolysis of olive oil obeyed the Michaelis-Menten rate equation. Values for Km = 924.9 mM and Vmax = 198.3 U/mg were lower than for free lipase, suggesting that the affinity towards the substrate changed and the activity of the immobilized lipase decreased during the course of immobilization. The immobilized derivative was also tested in the ester synthesis from several alcohols and carboxylic acids.

  6. Compactação de um latossolo vermelho causada pelo tráfego do "forwarder"

    Directory of Open Access Journals (Sweden)

    Fernandes Haroldo Carlos

    2003-01-01

    Full Text Available A mecanização tem sido, atualmente, um poderoso instrumento para suprimento das necessidades da indústria agroflorestal, estando presente nos processos de produção, colheita e transporte florestal. Esta utilização intensiva de máquinas pode, entretanto, acarretar danos ao solo, principalmente em termos de compactação, podendo refletir negativamente na produtividade. Tendo em vista a escassez de informações sobre a compactação do solo causada pelas máquinas florestais, o objetivo principal deste trabalho foi a avaliação dos níveis de compactação do solo causada pelo tráfego do trator florestal autocarregável (Forwarder. As variáveis físicas do solo analisadas foram a densidade e a resistência à penetração, tendo as amostras de solo sido retiradas em cinco pontos igualmente espaçados ao longo das trilhas, em três níveis de profundidades (0-15 cm, 15-30 cm e 30-50 cm. A análise estatística dos resultados de cada variável foi efetuada por meio da análise de variância para parcelas subdivididas, em que foram testados o efeito da máquina, o efeito da profundidade e o efeito da interação entre a máquina e a profundidade. A análise dos resultados obtidos permitiu constatar que o Forwarder provocou uma pequena compactação no solo, ou seja, incrementos de 0,06 g/cm³ na densidade e 1,00 MPa na resistência à penetração do solo. Os valores máximos da densidade do solo e resistência à penetração, depois da última passada da máquina, foram 1,12 g/cm³ e 2,87 Mpa, respectivamente.

  7. Inhibitory effect of acetic acid on bioconversion of xylose in xylitol by Candida guilliermondii in sugarcane bagasse hydrolysate Efeito inibitório do ácido acético na bioconversão de xilose em xilitol por Candida guilliermondii em hidrolisado de bagaço de cana

    Directory of Open Access Journals (Sweden)

    Débora D.V. Silva

    2004-09-01

    Full Text Available Sugarcane bagasse hydrolysate (initial acetic acid concentration = 3.5g/L, was used as a fermentation medium for conversion of xylose into xylitol by the yeast Candida guilliermondii FTI 20037. Acetic acid (2.0g/L was added to the medium at different times of fermentation, with the aim of evaluating its effects on the bioconversion process. The addition of acetic acid to the medium after 12h of fermentation resulted in the strongest inhibition of the yeast metabolism. In this case, the xylose consumption and cell growth were, respectively, 23.22 and 11.24% lower than when acid was added to the medium at the beginning of fermentation. As a consequence of the inhibitory effect, lower values of the xylitol yield (0.39g/g and productivity (0.22g/L.h were observed, corresponding to a reduction of 36 and 48%, respectively, in relation to the values obtained with the addition of acetic acid after other fermentation times. The results obtained allowed to conclude that, under the experimental conditions employed in this work, the inhibitory effect of acetic acid on the xylose-xylitol bioconversion depends on the fermentation time when this acid was added, and not only on its concentration in the medium.Hidrolisado de bagaço de cana-de-açúcar contendo uma concentração inicial de ácido acético de 3,5g/L foi utilizado como meio de fermentação para a bioconversão de xilose em xilitol pela levedura Candida guilliermondii FTI 20037. Ácido acético (2,0g/L foi adicionado ao meio em diferentes tempos de fermentação, com o objetivo de avaliar o efeito deste ácido neste bioprocesso. O maior efeito inibitório deste ácido na bioconversão de xilose em xilitol pela levedura ocorreu quando este foi adicionado ao meio após 12h de fermentação. Nesta condição observou-se uma redução de 23,22% e 11,24%, respectivamente, no consumo de xilose e no crescimento celular em relação à fermentação em que a adição deste ácido ocorreu no tempo inicial

  8. Abiotic alterations caused by forest fragmentation affect tree regeneration: a shade and drought tolerance gradient in the remnants of Coastal Maulino Forest Alteraciones abióticas causadas por la fragmentación del bosque afectan la regeneración arbórea: un gradiente de tolerancia a la sombra y la sequía en los remanentes del Bosque Maulino Costero

    Directory of Open Access Journals (Sweden)

    PABLO C GUERRERO

    2009-01-01

    by regeneration niche attributes for implementing conservation actions and ecological restoration.La regeneración en plantas está determinada por las diferencias de luz y humedad del suelo entre hábitats, ambas variables son modificadas por la fragmentación de bosques a gran escala. Varios estudios consideran esas alteraciones como parte del mecanismo involucrado en el cambio comunitario en bosques tropicales. Sin embargo, en bosques mediterráneos y deciduos esa tendencia podría ser más severa, debido a que las plantas presentan un "trade off" entre la tolerancia a la sombra y a la sequía. Nuestro estudio fue realizado en el Bosque Maulino Costero: Reserva Nacional Los Queules (RNLQ y fragmentos aledaños de bosque. Hipotetizamos que Aristotelia chilensis (sombra intolerante pero tolerante a la sequía debería aumentar su regeneración en fragmentos pequeños de bosque, como consecuencia del cambio en la idoneidad del habitat (i.e. más luminoso y seco, mientras que, Cryptocarya alba (sombra tolerante pero sequía intolerante debería reducir su regeneración en fragmentos pequeños de bosque. Nosotros además esperamos que Nothofagus glauca y N. obliqua, ambas con tolerancias a la sombra y a la sequía intermedias entre A. chilensis y C. alba, deberían responder en menor medida a la fragmentación del bosque. En este estudio utilizamos dos evaluaciones: (i la abundancia de plántulas y juveniles a través de observaciones de campo y (ii la germinación y establecimiento de plántulas vía un experimento de campo. Los patrones de regeneración natural de C. alba indican una reducción en la regeneración dentro de los fragmentos pequeños de bosque comparado con la RNLQ, a pesar de que en el experimento de campo, la proporción de semillas germinadas, de plántulas establecidas y reclutadas no variaron entre hábitats. Por el contrario, la regeneración de A. chilensis y de N. glauca fueron favorecidas por la fragmentación del bosque, con un aumento en las

  9. Genetic susceptibility to Candida infections

    NARCIS (Netherlands)

    Smeekens, S.P.; Veerdonk, F.L. van de; Kullberg, B.J.; Netea, M.G.

    2013-01-01

    Candida spp. are medically important fungi causing severe mucosal and life-threatening invasive infections, especially in immunocompromised hosts. However, not all individuals at risk develop Candida infections, and it is believed that genetic variation plays an important role in host susceptibility

  10. Espondilodiscitis y absceso epidural candidiásico Candida spondylodiscitis and epidural abscess

    Directory of Open Access Journals (Sweden)

    Gisela Di Stilio

    2006-08-01

    Full Text Available La espondilodiscitis candidiásica asociada a absceso epidural es una enfermedad de aparición excepcional. Se presenta el caso de un paciente con linfoma de Hodgkin en tratamiento quimioterápico que desarrolló candidiasis sistémica complicada con espondilodiscitis y absceso epidural por dicho germen.Candida spondylodiscitis associatd with epidural abscess is rarely seen. We present a patient with Hodgkin lymphoma who received chemotherapy and developed systemic Candida infection, which was complicated by Candida spondylodiscitis and epidural abscess.

  11. Pansinusitis y afectación intracraneal por implante dental Pansinusitis and intracranial impact of a dental implant

    OpenAIRE

    Josep Rubio-Palau; Jordi García-Linares; Javier Gutiérrez-Santamaría; Juan Antonio Hueto-Madrid; Mitchel Chávez-Gatty; Eduard Ferrés-Padró

    2012-01-01

    Las sinusitis odontógenas son una patología relativamente frecuente causada por infecciones dentales, quistes periapicales así como tras procedimientos bucodentales como una endodoncia, una elevación sinusal o la colocación de un implante. A continuación se presenta un caso extremo de una pansinusitis derecha con fistulización a espacio epidural causada por un implante osteointegrado. Ante la sospecha de una sinusitis maxilar de origen odontogénico se debe iniciar rápidamente un tratamiento a...

  12. Diagnósticos e atendimentos para surdocegos por rubéola congênita

    OpenAIRE

    Susana Maria Mana Aráoz

    2012-01-01

    Neste artigo, damos ênfase ao diagnóstico e posterior atendimento da surdocegueira causada por Rubéola Congênita. Destacamos as particularidades inerentes a este quadro em âmbito geral, através do uso de pesquisa para este fim.

  13. La responsabilidad del Estado por la actividad del legislador

    OpenAIRE

    María Consuelo Alonso García; Eric Leiva Ramírez

    2012-01-01

    Analizar el fenómeno de la responsabilidad del Estado causada por los hechos del legislador ha sido un trabajo desarrollado tanto por la doctrina y la jurisprudencia internacional a mediados del siglo xx. En Francia, por ejemplo, se presentó el primer antecedente jurisprudencial en el año de 1934. En España, su estudio empezó a gestionarse con la promulgación de la Constitución de 1978, presentándose el primer fallo judicial en el año de 1993. Para el caso colombiano, la Constitución de 1991 ...

  14. Pathogenesis of Candida vulvovaginitis.

    Science.gov (United States)

    Sobel, J D

    1989-01-01

    The occurrence of candida vulvovaginitis (CVV) has been estimated based on statistical data from Great Britain to be an increase to 200/100,000 over 10 years to 1984. CVV in the US is the 2nd commonest cause of vaginal infection, with bacterial vaginosis occurring twice as often. 85-90% of the yeasts isolated from the vagina are candida albicans, based on biotyping rather that the newer methods of DNA hybridization. The pathogenesis of CVV is discussed in terms of the microbiology (virulence factors, adherence, germ tube and mycelium formation, proteinase secretion, and switching colonies), asymptomatic vaginal colonization, transformation to symptomatic vaginitis, host predisposing factors (pregnancy, oral contraceptives, diabetes mellitus, antimicrobes, and other), vaginal defense mechanisms (humoral system, phagocytic system, cell mediated immunity, vaginal flora, other), and pathogenesis of recurrent and chronic CVV (internal reservoir, sexual transmission, vaginal relapse, and experimental models) The discussion of the development of virulent symptoms is capsuled in the following comments. Vaginal cell receptivity varies among individuals, but all strains of C. Albicans adhere to both exfoliated vaginal and buccal epithelial cells, or mucosal surfaces, through the yeast surface mannoprotein. It is suggested from in vitro studies that germ tube and mycelium formation facilitates vaginal mucosal invasion. Exogenous and endogenous factors may enhance germination and precipitate symptomatic vaginitis, or inhibit germination. Increased proteinase secretion may be a result of the transformation from the blastoconidium/colonization phase to the germinated invasive vaginitis stage or an independent virulence factor. It is reported that hereditable spontaneous switching may occur spontaneously in vivo also. Colonizing yeasts with a change in environment can transform to a more virulent phase. Colonization rates vary from 10-25%, and the critical issue is understanding

  15. EPIDEMIOLOGICAL ASPECTS OF LEAF BLIGHT OF CARROT (Daucus carota L. CAUSED BY Alternaria dauci (KÜHN GROVES & SKOLKO: SURVIVAL OF Alternaria dauci IN VEGETAL RESIDUES OF CARROT (Daucus carota L. CROP ASPECTOS EPIDEMIOLÓGICOS DA QUEIMA DAS FOLHAS DA CENOURA (Daucus carota L. CAUSADA POR Alternaria dauci (Kühn GROVES & SKOLKO: Sobrevivência de Alternaria dauci (Kühn Groves & SKOLKO em restos culturais da cenoura (Daucus carota L.

    Directory of Open Access Journals (Sweden)

    Geraldo Martins Chaves

    2007-09-01

    Full Text Available

    The spore viability of Alternaria dauci (Kühn Groves & Skolko in carrot (Daucus carota L. debris which were kept at different levels of temperature and relative humidity, without light, was studied. The temperature levels tested were 8, 16, 24 and 32°C, and the relative humidity levels 3, 18, 51, 73 and 100%. The treatments were different combinations of these levels. Humidity was the more important factor in preserving the fungus spore ability to germinate and its infectivity. Under conditions of high relative humidity and temperatures of 24 and 32°C, the spores rapidly lost their viability. This did not happen when the relative humidity was equal to, or below, 51%. The survival of fungus mycelium on carrot petioles was studied in non-treated organic soil, at different humidity levels, at three different depths, during a period of 120 days. Soil humidity was the factor of major significance in the persistence of the mycelium, followed by depths and time factors. In petioles kept at depths of 10 and 20 cm in humid soil, the survival was greatly reduced, while survival was markedly higher in those kept at the surface of dry soil. The effect of humidity on the viability of mycelium was studied also under soiless condition. Petioles were maintained in relative humidity controlled chamber, at 24°C. In saturated atmosphere (100% humidity, the mycelium viability was lost in less than 20 days. It was concluded that both, the conidial and mycelial form of Alternaria dauci are quite sensitive to high humidity levels.

    Estudou-se a sobrevivência das formas conidial e miceliana de Alternaria dauci, respectivamente, sobre folhas necrosadas e pecíolos de cenoura, sob diferentes condições mesológicas. Os esporos do fungo sobre conidióforos em folhas necrosadas de cenoura foram mantidos por 120 dias sob condi

  16. Genetic susceptibility to Candida infections

    OpenAIRE

    Smeekens, S P; Veerdonk, F.L. van de; Kullberg, B J; Netea, M.G.

    2013-01-01

    Candida spp. are medically important fungi causing severe mucosal and life-threatening invasive infections, especially in immunocompromised hosts. However, not all individuals at risk develop Candida infections, and it is believed that genetic variation plays an important role in host susceptibility. On the one hand, severe fungal infections are associated with monogenic primary immunodeficiencies such as defects in STAT1, STAT3 or CARD9, recently discovered as novel clinical entities. On the...

  17. Eficacia in vitro de la moxifloxacina frente a Candida albicans en enfermedad periodontal

    Directory of Open Access Journals (Sweden)

    C.M. Ardila Medina

    2014-04-01

    Full Text Available Introducción: La periodontitis crónica está asociada a una compleja y diversa flora microbiana que incluye posiblemente especies de Candida. Objetivo: Evaluar la presencia de Candida y su susceptibilidad in vitro a la moxifloxacina en pacientes con periodontitis crónica. Materiales y métodos: Se usó una técnica de diluciones seriales para evaluar la susceptibilidad de la Candida a la moxifloxacina. Se utilizó el porcentaje de individuos con al menos una bolsa infectada para describir la presencia de individuos positivos a especies de Candida. Resultados y conclusiones: se detectaron especies de Candida en 13,2% de 76 pacientes con periodontitis crónica. La moxifloxacina inhibió significativamente el crecimiento de Candida (90%. La moxifloxacina puede ser una buena alternativa para el tratamiento de Candida debido a que en situaciones específicas la sobreinfección por este microorganismo puede responder inadecuadamente a tratamientos periodontales convencionales.

  18. Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina.

    Science.gov (United States)

    Khunnamwong, Pannida; Lertwattanasakul, Noppon; Jindamorakot, Sasitorn; Limtong, Savitree; Lachance, Marc-André

    2015-12-01

    Three strains (DMKU-RE28, DMKU-RE43T and DMKU-RE123) of a novel anamorphic yeast species were isolated from rice leaf tissue collected in Thailand. DNA sequence analysis demonstrated that the species forms a sister pair with Candida ranongensis CBS 10861T but differs by 24-30 substitutions in the LSU rRNA gene D1/D2 domains and 30-35 substitutions in the ITS region. A phylogenetic analysis based on both the small and the large rRNA gene subunits confirmed this connection and demonstrated the presence of a clade that also includes Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida rugosa and Candida scorzettiae. The clade is not closely affiliated to any known teleomorphic genus, and forms a well-separated lineage from currently recognized genera of the Saccharomycetales. Hence, the genus Diutina gen. nov. is proposed to accommodate members of the clade, including Diutina siamensis f.a. sp. nov. and the preceding seven Candida species. The type strain is DMKU-RE43T ( = CBS 13388T = BCC 61183T = NBRC 109695T). PMID:26410375

  19. Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate

    OpenAIRE

    Apurva K Pathak; Sanjay Sharma; Pallavi Shrivastva

    2012-01-01

    OBJECTIVE: In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. MATERIAL AND METHODS: The species of Candida, isolated from multiple species oral...

  20. HIPERAMONEMIA NEONATAL CAUSADA POR DEFECTOS DEL CICLO DE LA UREA Neonatal hyperammonemia in urea cycle disorders patients

    Directory of Open Access Journals (Sweden)

    Yolanda Cifuentes C

    2010-12-01

    Full Text Available Los defectos del ciclo de la úrea se deben a deficiencias de diferentes enzimas; las manifestaciones clínicas son similares y están relacionadas con la hiperamonemia. Se presentan las historias clínicas de tres neonatos a término, sin evidencia de alteración al nacimiento. Se les detectó hiperamonemia y se sospechó enfermedad metabólica. La cromatografía de aminoácidos sugirió defectos del ciclo de la úrea. El manejo incluyó dieta con restricción de proteínas, administración de benzoato de sodio, exsanguinotransfusión y diálisis peritoneal pese a lo cual fallecieron. Se revisan las causas de hiperamonemia en el neonato y se propone una secuencia para su diagnósticoThe urea cycle disorders result from deficiency of activity of enzymes N-acetyl glutamate synthetase, carbamyl phosphate synthase, ornithine transcarbamylase, argininosuccinic acid synthetase, argininosuccinic acid lyase and arginase. Except for the last one, the clinical features are similar and related with the hiperammonaemia. It reports three full term, newborn cases, they had encephalopathy and needed respiratory support after be well in neonatal period. They had hyperammonemia as inborn error. The thin layer amino acids chromatography showed alanine and glutamine, in the siblings appeared citruline, suggesting urea cycle disorders. Despite protein restriction diet, sodium benzoate administration, blood exchange and peritoneal dialysis,babies died. High argininosuccinic acid levels in the first case and high citrulline levels with argininosuccinic acid absence in the third case, which was diagnosed as argininosuccinic aciduria with citrullinemia. This report provide an overview of neonatal hyperammonemia causes and propose a secuency for diagnosis

  1. Avaliação da resistência do eucalipto à murcha-bacteriana causada por Ralstonia solanacearum

    OpenAIRE

    Reginaldo Gonçalves Mafia; Acelino Couto Alfenas; Maria Alves Ferreira

    2014-01-01

    O plantio de clones de eucalipto resistentes constitui a principal estratégia para o controle de doenças no campo. Assim, este trabalho objetivou testar métodos de inoculação de Ralstonia solanacearum visando selecionar eucalipto resistente à murcha-bacteriana. Os métodos de inoculação foram selecionados em função da facilidade operacional e testados na avaliação de quatro clones (híbridos E. urophylla x E. grandis): i) aplicação de 5 mL de inóculo (10(8) ufc/mL) na região do coleto de mudas;...

  2. Puberdade precoce causada por tumores intracranianos: relato de 4 casos Pubertas praecox due to intracranial tumors: report of 4 cases

    Directory of Open Access Journals (Sweden)

    Reynaldo A. Brandt

    1974-12-01

    Full Text Available São relatados 4 casos de puberdade precoce conseqüentes a tumores intracranianos: um teratoma adulto do ventrículo lateral, um astrocitoma protoplasmático do terceiro ventrículo e diencéfalo, um astroblastoma do ter- ceiro ventrículo e diencéfalo e um hamartoma hipotalâmico. São apresentadas teorias que tentam explicar os mecanismos hormonais envolvidos e é salientada a importância do diagnóstico neuroradiológico preciso, pela possibilidade de cura em alguns casos e de tratamento satisfatório nos demais.Four cases of precocious puberty secondary to intracranial tumors are reported. A 3 year and 11 month old boy had a teratoma in the left lateral ventricle and total surgical excision was followed by recovery. An 8 year old girl with a protoplasmic astrocytoma of the third ventricle diagnosed by biopsy, followed by shunting and cobalt therapy was discharged improved. A five year old boy with an extensive astroblastoma of the diencephalon died following biopsy and shunting of cerebrospinal fluid. A posterior hypothalamic hamartoma totally excised from a 3 year old girl was followed by remission of the precocious puberty. The theories of hormonal mechanisms leading to precocious puberty are presented. It is stressed the importance of pneumoencephalography in the diagnosis of intracranial hamartomas, for they lack neurological signs.

  3. Cervical celullitis in broiler chickens for Escherichia coli/ Celulite cervical em frangos de corte causada por Escherichia coli

    Directory of Open Access Journals (Sweden)

    Ivens Gomes Guimarães

    2002-05-01

    Full Text Available In this paper was report the isolation of Escherichia coli in broiler chickens with cellulitis in the cervical region. It was carried through the isolation of E. coli of the lesion of cellulitis from broilers and carried through histopathological examination of skin that had characterized the lesion. Focal ulcerations of epidermis, fibrin in dermis and difuse infiltrated by lymphocytes and heterophils on subcutaneous tissues.Neste trabalho, relata-se o isolamento de Escherichia coli em frangos de corte apresentando lesão de celulite na região cervical. Foi realizado o isolamento de E. coli da lesão de celulite e realizado exames histopatológicos que caracterizaram a lesão. Na epiderme foram verificadas lesões ulcerativas, presença de fibrina na derme e infiltração difusa de linfócitos e heterófilos no tecido subcutâneo.

  4. Cervical celullitis in broiler chickens for Escherichia coli/
    Celulite cervical em frangos de corte causada por Escherichia coli

    OpenAIRE

    Ivens Gomes Guimarães; Milene Martins Berbel; Marcia Marinho Luzardo; Werner Okano; Claudia Yurika Tamehiro; Benito Guimarães de Brito

    2002-01-01

    In this paper was report the isolation of Escherichia coli in broiler chickens with cellulitis in the cervical region. It was carried through the isolation of E. coli of the lesion of cellulitis from broilers and carried through histopathological examination of skin that had characterized the lesion. Focal ulcerations of epidermis, fibrin in dermis and difuse infiltrated by lymphocytes and heterophils on subcutaneous tissues.Neste trabalho, relata-se o isolamento de Escherichia coli em frango...

  5. Jejunal perforation caused by abdominal angiostrongyliasis Perfuração jejunal causada por angiostrongilíase abdominal

    OpenAIRE

    Jaques WAISBERG; Carlos Eduardo CORSI; Marisa Valente REBELO; Vilma Therezinha Trench VIEIRA; Sansom Henrique BROMBERG; Paulo Amaral dos SANTOS; Monteiro, Rodrigo

    1999-01-01

    The authors describe a case of abdominal angiostrongyliasis in an adult patient presenting acute abdominal pain caused by jejunal perforation. The case was unusual, as this affliction habitually involves the terminal ileum, appendix, cecum or ascending colon. The disease is caused by the nematode Angiostrongylus costaricensis, whose definitive hosts are forest rodents while snails and slugs are its intermediate hosts. Infection in humans is accidental and occurs via the ingestion of snail or ...

  6. Candida famata (Debaryomyces hansenii)

    Science.gov (United States)

    Sibirny, Andriy A.; Voronovsky, Andriy Y.

    Debaryomyces hansenii (teleomorph of asporogenous strains known as Candida famata ) belongs to the group of so named ‘ flavinogenic yeasts ’ capable of riboflavin oversynthesis during starvation for iron. Some strains of C. famata belong to the most flavinogenic organisms known (accumulate 20 mg of riboflavin in 1 ml of the medium) and were used for industrial production of riboflavin in USA for long time. Many strains of D. hansenii are characterized by high salt tolerance and are used for ageing of cheeses whereas some others are able to convert xylose to xylitol, anti-caries sweetener. Transformation system has been developed for D. hansenii. It includes collection of host recipient strains, vectors with complementation and dominant markers and several transformation protocols based on protoplasting and electroporation. Besides, methods of multicopy gene insertion and insertional mutagenesis have been developed and several strong constitutive and regulatable promoters have been cloned. All structural genes of riboflavin synthesis and some regulatory genes involved in this process have been identified. Genome of D. hansenii has been sequenced in the frame of French National program ‘Genolevure’ and is opened for public access

  7. Urinary tract infections and Candida albicans

    OpenAIRE

    BEHZADI, Payam; BEHZADI, Elham; Ranjbar, Reza

    2015-01-01

    Introduction Urinary tract candidiasis is known as the most frequent nosocomial fungal infection worldwide. Candida albicans is the most common cause of nosocomial fungal urinary tract infections; however, a rapid change in the distribution of Candida species is undergoing. Simultaneously, the increase of urinary tract candidiasis has led to the appearance of antifungal resistant Candida species. In this review, we have an in depth look into Candida albicans uropathogenesis and distribution o...

  8. Comparison of the extracellular polymeric substances of Candida albicans and Candida dubliniensis biofilms

    OpenAIRE

    Henriques, Mariana; M.C. Fernandes; Azeredo, Joana; Oliveira, Rosário

    2006-01-01

    Candida albicans and Candida dubliniensis live as benign commensal organisms in the oral cavity of both healthy and unhealthy individuals behaving, under certain conditions, as opportunistic pathogens, causing candidiasis. These two Candida species have been mismatched for years, but recently Candida dubliniensis was recovered from the mouth of imunnosupressed patients and identified as a different species. Candidiasis is usually related with the Candida capacity of forming biofilms on inert ...

  9. Farnesol : beyond morphogenesis control in non-candida albicans candida species

    OpenAIRE

    Martins, M.; Henriques, Mariana; Azeredo, Joana; Oliveira, Rosário

    2007-01-01

    During the last decade the incidence of candidiasis increased dramatically. Although Candida albicans remains the most frequent cause of infections, non-Candida albicans candida (NCAC) species are emerging as new pathogens. Candida infections are often associated with biofilms that can develop on natural surfaces and medical devices. In a similar manner to other microorganisms, signalling pathways may control the diversity and distribution of Candida species within biofilms. E,...

  10. Effect of Candida albicans and Candida dubliniensis planktonic/biofilm quorum sensing molecules on yeast morphogenesis

    OpenAIRE

    Henriques, Mariana; Martins, M.; Azeredo, Joana; Oliveira, Rosário

    2006-01-01

    One of the aims of this work was to study the effect of farnesol, a quorum sensing molecule for Candida albicans, on morphologic inhibition of Candida dubliniensis. The second goal of this work was to confirm if Candida dubliniensis also excreted quorum sensing molecules, on both planktonic and biofilm forms. The results clearly demonstrate that Candida dubliniensis undergoes morphological alterations triggered by farnesol. It was also found that supernatants of Candida dubliniensis and Ca...

  11. Candida in mouth or on dummy?

    OpenAIRE

    Manning, D J; Coughlin, R P; Poskitt, E M

    1985-01-01

    Mouth and dummy swabs for Candida spp. were obtained from 100 children under 18 months old admitted with acute medical conditions. Forty four per cent of dummies were colonised by Candida spp. Children who sucked dummies had clinical thrush and positive mouth swabs for candida more frequently than those who did not.

  12. [An update on Candida dubliniensis].

    Science.gov (United States)

    Paugam, A; Baixench, M-T; Viguié, C

    2008-01-01

    Eleven years ago, Irish authors, using molecular biology, demonstrated the existence of Candida dubliniensis, a new species of Candida close to Candida albicans. Initially isolated from AIDS patients with oral candidiasis, this species was detected, even in immunocompetent patients. Recently, with new, easy to implement identification tests (latex, immunochromatography), numerous epidemiological studies were undertaken. In most studies, C. dubliniensis was most often identified in the oral cavity. In the absence of HIV infection, the proportion C. dubliniensis/C. albicans ranged from 1 to 5% but it increased to 15-20% in case of HIV infection. It should be stressed that, from an experimental point of view, the acquisition of a secondary resistance to fluconazole is more quickly obtained with C. dubliniensis that with C. albicans, this resistance remains exceptionally observed in clinical observations. PMID:18065177

  13. Genetic susceptibility to Candida infections.

    Science.gov (United States)

    Smeekens, Sanne P; van de Veerdonk, Frank L; Kullberg, Bart Jan; Netea, Mihai G

    2013-06-01

    Candida spp. are medically important fungi causing severe mucosal and life-threatening invasive infections, especially in immunocompromised hosts. However, not all individuals at risk develop Candida infections, and it is believed that genetic variation plays an important role in host susceptibility. On the one hand, severe fungal infections are associated with monogenic primary immunodeficiencies such as defects in STAT1, STAT3 or CARD9, recently discovered as novel clinical entities. On the other hand, more common polymorphisms in genes of the immune system have also been associated with fungal infections such as recurrent vulvovaginal candidiasis and candidemia. The discovery of the genetic susceptibility to Candida infections can lead to a better understanding of the pathogenesis of the disease, as well as to the design of novel immunotherapeutic strategies. This review is part of the review series on host-pathogen interactions. See more reviews from this series. PMID:23629947

  14. Onycholysis caused by Candida Krusei

    Directory of Open Access Journals (Sweden)

    Rao S

    2004-01-01

    Full Text Available Onycholysis caused by Candida krusei is rare. A 21 years old male patient presented with grayish discolouration and elevation of all fingernails since one year. Patient was refractory to treatment with fluconazole. Potassium hydroxide preparation of subungual debris revealed fungal elements. Growth on Sabouraud dextrose agar was identified by cultural characteristics, morphotyping, microscopy and biochemical tests as Candida krusei. The isolate was resistant to fluconazole and amphotericin-B but susceptible to nystatin and clotrimazole. Patient responded well to clotrimazole and terbinafine.

  15. Acetiltransferasas de histonas en Candida albicans: análisis del perfil transcripcional

    OpenAIRE

    Dégano Blázquez, Rosa María

    2011-01-01

    [ES] Candida albicans es un hongo oportunista capaz de crecer adoptando diferentes morfologías según las condiciones del medio por lo que además resulta útil como modelo en estudios de diferenciación molecular.

  16. Molecular screening for Candida orthopsilosis and Candida metapsilosis among Danish Candida parapsilosis group blood culture isolates: proposal of a new RFLP profile for differentiation

    DEFF Research Database (Denmark)

    Mirhendi, Hossein; Bruun, Brita; Schønheyder, Henrik Carl;

    2010-01-01

    Candida orthopsilosis and Candida metapsilosis are recently described species phenotypically indistinguishable from Candida parapsilosis . We evaluated phenotyping and molecular methods for the detection of these species among 79 unique blood culture isolates of the C. parapsilosis group obtained...

  17. Synthetic arylquinuclidine derivatives exhibit antifungal activity against Candida albicans, Candida tropicalis and Candida parapsilopsis

    Directory of Open Access Journals (Sweden)

    Gilbert Ian

    2011-01-01

    Full Text Available Abstract Background Sterol biosynthesis is an essential pathway for fungal survival, and is the biochemical target of many antifungal agents. The antifungal drugs most widely used to treated fungal infections are compounds that inhibit cytochrome P450-dependent C14α-demethylase (CYP51, but other enzymes of this pathway, such as squalene synthase (SQS which catalyses the first committed step in sterol biosynthesis, could be viable targets. The aim of this study was to evaluate the antifungal activity of SQS inhibitors on Candida albicans, Candida tropicalis and Candida parapsilopsis strains. Methods Ten arylquinuclidines that act as SQS inhibitors were tested as antiproliferative agents against three ATCC strains and 54 clinical isolates of Candida albicans, Candida tropicalis and Candida parapsilopsis. Also, the morphological alterations induced in the yeasts by the experimental compounds were evaluated by fluorescence and transmission electron microscopy. Results The most potent arylquinuclidine derivative (3-[1'-{4'-(benzyloxy-phenyl}]-quinuclidine-2-ene (WSP1267 had a MIC50 of 2 μg/ml for all species tested and MIC90 varying from 4 μg/ml to 8 μg/ml. Ultrathin sections of C. albicans treated with 1 μg/ml of WSP1267 showed several ultrastructural alterations, including (a loss of cell wall integrity, (b detachment of the plasma membrane from the fungal cell wall, (c accumulation of small vesicles in the periplasmic region, (d presence of large electron-dense vacuoles and (e significantly increased cell size and cell wall thickness. In addition, fluorescence microscopy of cells labelled with Nile Red showed an accumulation of lipid droplets in the cytoplasm of treated yeasts. Nuclear staining with DAPI revealed the appearance of uncommon yeast buds without a nucleus or with two nuclei. Conclusion Taken together, our data demonstrate that arylquinuclidine derivatives could be useful as lead compounds for the rational synthesis of new

  18. Antifungigrama para comprovar o potencial de ação dos extratos vegetais hidroglicólicos sobre Candida sp. (Berkhout) Etest to confirm the action potential of plant hydroglycol extracts on Candida sp. (Berkhout)

    OpenAIRE

    E.A.V. Glehn; G.P.S. Rodrigues

    2012-01-01

    A candidíase vaginal é uma doença causada, na maioria das vezes, pelo fungo do gênero Candida sp, que habita o trato gastrintestinal e geniturinário da espécie humana e pode tornar-se patogênico sob determinadas condições. A maioria dos indivíduos desenvolve defesas imunológicas que impedem a proliferação e desenvolvimento de candidíase localizada ou disseminada. Embora a causa exata do aumento de espécies não-albicans seja desconhecida, há evidências de que a própria terapia antifúngica poss...

  19. Sepsis neonatal por Estreptococos Pyogenes

    Directory of Open Access Journals (Sweden)

    Gilberto Rodríguez-Herrera

    2009-09-01

    Full Text Available Se presenta el caso de un paciente masculino, recién nacido a término adecuado para la edad gestacional, quien nace por parto vaginal, con el antecedente de fiebre en la madre durante el periodo de postparto inmediato. Los padres consultan a los 2 días de vida pues le notan dificultad respiratoria, hipoactividad y rechazo a la leche materna. El paciente se interna y se aborda como una sepsis. Durante su estancia en el servicio de neonatología del Hospital Nacional de Niños asocia fallo respiratorio que amerita ventilación mecánica asistida por varios días en diferentes ocasiones, derrame pleural exudativo, convulsiones de origen hipóxico isquémico. Con reporte de hemocultivos positivos por Estreptococos pyogenes. El Estreptococos pyogenes o estreptococo β-hemolνtico del grupo A, fue un problema en los comienzos del siglo pasado, siendo frecuente en las infecciones puerperales y del reciιn nacido. En la actualidad es un germen sumamente raro en los procesos de sepsis neonatal.2 La gravedad de la enfermedad causada por este microorganismo en el periodo neonatal varνa desde una onfalitis crónica de bajo grado a una septicemia, una meningitis fulminante y la muerte.1 El presente artículo pretende hacer un resumen del paciente, con su evolución clínica, radiológica y además ejemplificar todas las complicaciones que tuvimos con este germen tan poco frecuente en la actualidad en sepsis neonatal.

  20. Alterações na dispersão de sementes por carnívoros causadas por alterações no habitat

    OpenAIRE

    Teixeira, Isa Sofia de Sá, 1990-

    2014-01-01

    Tese de mestrado. Biologia (Ecologia e Gestão Ambiental). Universidade de Lisboa, Faculdade de Ciências, 2014 A dispersão de sementes é um processo de ecossistema fundamental que determina a distribuição espacial, a expansão e a manutenção das populações e comunidades vegetais. Diversos grupos taxonómicos cumprem o papel de dispersores de sementes, entre os quais os mamíferos carnívoros, particularmente importantes em ecossistemas mediterrâneos. O montado é um sistema agro-florestal, inte...

  1. Occurrence and Diversity of Candida Genus in Marine Environments

    Institute of Scientific and Technical Information of China (English)

    WANG Lin; CHI Zhenming; YUE Lixi; CHI Zhe; ZHANG Dechao

    2008-01-01

    A total of 317 yeast isolates from seawater,sediments,mud of salterns,guts of marine fishes and marine algae wereobtained.The results of routine identification and molecular characterization showed that six isolates among these marine yeastsbelonged to Candida genus as Candida interrnedia for YA01a,Candida parapsilosis for 3eA2,Candida quercitrusa for JHSb,Can-die rugosa for wl8,Candida zeylanoides for TJY13a,and Candida membranifaciens for W14-3.Isolates YA01a (Candida interme-die),wl8 (Candida rugosa),3eA2 (Candida parapsilosis),and JHSb (Candida quercitrusa) were found producing cell-bound lipase,while isolate W14-3 (Candida membranifaciens) producing riboflavin.These marine yeast Candida spp.Seem to have wide potentialapplications in biotechnology.

  2. The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces

    NARCIS (Netherlands)

    T. Pereira-Cenci; D.M. Deng; E.A. Kraneveld; E.M.M. Manders; A.A. Del Bel Cury; J.M. ten Cate; W. Crielaard

    2008-01-01

    Although Candida containing biofilms contribute to the development of oral candidosis, the characteristics of multi-species Candida biofilms and how oral bacteria modulate these biofilms is poorly understood. The aim of this study was to investigate interactions between Candida albicans and either C

  3. Penetration of Candida Biofilms by Antifungal Agents

    OpenAIRE

    Al-Fattani, Mohammed A.; Douglas, L. Julia

    2004-01-01

    A filter disk assay was used to investigate the penetration of antifungal agents through biofilms containing single and mixed-species biofilms containing Candida. Fluconazole permeated all single-species Candida biofilms more rapidly than flucytosine. The rates of diffusion of either drug through biofilms of three strains of Candida albicans were similar. However, the rates of drug diffusion through biofilms of C. glabrata or C. krusei were faster than those through biofilms of C. parapsilosi...

  4. Candida Infections of the Genitourinary Tract

    OpenAIRE

    Achkar, Jacqueline M.; Fries, Bettina C.

    2010-01-01

    Summary: All humans are colonized with Candida species, mostly Candida albicans, yet some develop diseases due to Candida, among which genitourinary manifestations are extremely common. The forms of genitourinary candidiasis are distinct from each other and affect different populations. While vulvovaginal candidiasis affects mostly healthy women, candiduria occurs typically in elderly, hospitalized, or immunocompromised patients and in neonates. Despite its high incidence and clinical relevan...

  5. Susceptibility characterisation of Candida spp. to four essential oils

    OpenAIRE

    Rath, C. C.; Mohapatra, S.

    2015-01-01

    In the present investigation, anti-Candida activity of four essential oils i.e. Black cumin (Nigella sativa), Curry leaf (Murraya koienigii), Ajwain (Trachiyspirum ammi), and Betel leaf (Piper betel) were screened against four human pathogenic species of Candida viz. Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. The minimum inhibitory concentration (MIC) values of the oils ranged between 15.62 and 250 μl/ml while studied through tube dilution method. The oi...

  6. GAp permeases in Candida albicans

    Czech Academy of Sciences Publication Activity Database

    Kraidlová, Lucie; Sychrová, Hana; Van Dijck, P.

    Fyziologický ústav AV ČR, v. v. i.. Roč. 57, č. 4 (2008), 79P-79P ISSN 0862-8408. [PhD Student Workshop of Institute of Physiology. 02.06.2008-04.06.2008, Seč] R&D Projects: GA MŠk(CZ) LC531 Institutional research plan: CEZ:AV0Z50110509 Keywords : cpr1 * Candida albicans * amino-acid uptake * GAP permease Subject RIV: EE - Microbiology, Virology

  7. Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos

    Directory of Open Access Journals (Sweden)

    Alessandra S. Belo Reis

    2011-09-01

    Full Text Available Foram realizadas pesquisas sobre a natureza e causa de lesões de pele em equídeos em uma propriedade no município de Castanhal, região Nordeste do Estado do Pará. Foram realizadas visitas técnicas, estudos epidemiológicos, coletas de sangue, biópsias de pele afetada e a inspeção da pastagem. O estudo incluiu 25 equídeos, dos quais 14 machos e 11 fêmeas, de seis meses e oito anos de idade. Os animais apresentaram lesões ulcerativas, de bordos irregulares, na cabeça (narinas, focinho, lábios superiores e inferiores e chanfro, na cavidade oral (vestíbulo bucal e gengiva e nos membros (boletos, metacarpos e metatarsos e articulação escápulo-umeral. No exame histopatológico foram observados focos de erosões cutâneas, caracterizados por perda e necrose da epiderme, com espongiose, degeneração vesicular da epiderme remanescente e leve infiltrado inflamatório na derme subjacente, constituído predominantemente por macrófagos e, em menor grau, eosinófilos. Na inspeção da pastagem, constituída de Brachiaria humidicola, foi constatada grande invasão de duas plantas providas de espinhos, Mimosa pudica e Mimosa debilis, ambas da família Leg. Mimosoideae. Concluiu-se, que as lesões de pele foram causadas pela ação traumática dos espinhos de Mimosa pudica e Mimosa debilis.

  8. Estudo da protecção de lesões dopaminérgicas exercidas por meios condicionados por células estaminais e por astrócitos

    OpenAIRE

    Parente, Ana Rita Nunes

    2010-01-01

    A doença de Parkinson (DP) é causada por uma progressiva e selectiva perda de neurónios dopaminérgicos mesencefálicos da via nigroestriatal. Para além da perda de neurónios dopaminérgicos, inclusões proteicas intraneurais citoplasmáticas denominadas por “Corpos de Lewy”, contendo α-sinucleína e ubiquitina, são também características desta patologia. Os mecanismos moleculares e celulares responsáveis pelo desenvolvimento da DP não se encontram totalmente compreendidos. Estudos anteriores suger...

  9. Evaluation of Bichro-Dubli Fumouze to distinguish Candida dubliniensis from Candida albicans.

    Science.gov (United States)

    Sahand, Ismail H; Moragues, María D; Robert, Raymond; Quindós, Guillermo; Pontón, José

    2006-06-01

    We have evaluated the ability of the Bichro-Dubli Fumouze (Fumouze Diagnostics, Levallois-Perret, France) latex agglutination test to identify colonies of Candida dubliniensis grown on different media. The test was positive for 103 of 106 isolates of C. dubliniensis and negative for Candida albicans and other Candida species studied. The sensitivity and specificity of the test were 97.1% and 100%, respectively. The test is very rapid, simple, and reliable giving the same results independently of whether the colonies are grown previously on Sabouraud dextrose agar, CHROMagar Candida medium, Candida ID2 medium, or CHROMagar-Pal's medium. PMID:16529902

  10. Papel da oxidase alternativa na resistência ao stress oxidativo em Candida krusei

    OpenAIRE

    Barbosa, Matilde de Oliveira

    2008-01-01

    Tal como Candida albicans, C. krusei está associada a infecções fúngicas invasivas em indivíduos imunodeprimidos por SIDA ou por quimioterapia. Estas infecções fúngicas invasivas têm assumido particular relevância dado que na sua grande maioria se observam casos de resistência ao fluconazol e outros azois. Por outro lado, os mecanismos de patogenicidade de C. krusei não estão documentados sabendo-se que não se relacionam com os descritos para C. albicans. Na mitocôndria de todas as c...

  11. Raza:factor de riesgo para el desarrollo de la fiebre hemorrágica por dengue:implicación de la respuesta inmune celular

    OpenAIRE

    Sierra Vázquez, Beatriz de la Caridad

    2011-01-01

    La enfermedad por virus dengue es la más importante enfermedad viral transmitida por artrópodos. Es causada por uno de cuatro serotipos del virus y produce un espectro clínico que va desde formas asintomáticas, pasando por formas leves de fiebre clásica (Fiebre dengue: FD) hasta las formas más severas de Fiebre Hemorrágica del Dengue/Síndrome de Choque por Dengue (FHD/SCD). El análisis de las epidemias cubanas de dengue ha permitido detectar varios factores de riesgo asociados a la susceptibi...

  12. Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA.

    OpenAIRE

    Williams, D W; Wilson, M. J.; Lewis, M. A.; Potts, A J

    1995-01-01

    The PCR was used to amplify a targeted region of the ribosomal DNA from 84 Candida isolates. Unique product sizes were obtained for Candida guilliermondii, Candida (Torulopsis) glabrata, and Candida pseudotropicalis. Isolates of Candida albicans, Candida tropicalis, Candida stellatoidea, Candida parapsilosis, and Candida krusei could be identified following restriction digestion of the PCR products.

  13. Novel strategies to fight Candida species infection.

    Science.gov (United States)

    Rodrigues, Maria Elisa; Silva, Sónia; Azeredo, Joana; Henriques, Mariana

    2016-08-01

    In recent years, there has been a significant increase in the incidence of human fungal infections. The increase in cases of infection caused by Candida species, and the consequent excessive use of antimicrobials, has favored the emergence of resistance to conventional antifungal agents over the past decades. Consequently, Candida infections morbidity and mortality are also increasing. Therefore, new approaches are needed to improve the outcome of patients suffering from Candida infections, because it seems unlikely that the established standard treatments will drastically lower the morbidity of mucocutaneous Candida infections and the high mortality associated with invasive candidiasis. This review aims to present the last advances in the traditional antifungal therapy, and present an overview of novel strategies that are being explored for the treatment of Candida infections, with a special focus on combined antifungal agents, antifungal therapies with alternative compounds (plant extracts and essential oils), adjuvant immunotherapy, photodynamic therapy and laser therapy. PMID:25383647

  14. Toxicidade ocular causada pelo tamoxifeno: relato de caso Ocular toxicity caused by tamoxifen: case report

    Directory of Open Access Journals (Sweden)

    Eliane Terumi Inada

    2005-08-01

    Full Text Available O objetivo desse trabalho é relatar um caso de toxicidade ocular pelo tamoxifeno. Para isso, aferiu-se a melhor acuidade visual corrigida de ambos os olhos em tabela de Snellen. Foram realizados biomicroscopia do segmento anterior, refração, oftalmoscopia, angiofluoresceinografia e retinografia numa paciente de 63 anos, sexo feminino, cor branca, em uso de tamoxifeno 20 mg/dia há 4 anos, com acuidade visual corrigida de 20/70 e 20/40. A biomicroscopia do segmento anterior apresentava ceratopatia verticilata e catarata nuclear e cortical posterior de 1+/4 em ambos os olhos. À oftalmoscopia, foi verificado alteração do brilho macular de ambos os olhos. E a angiofluoresceinografia mostrou hiperfluorescência na área macular em fase precoce (defeito em janela. Relata-se um caso de ceratopatia e maculopatia causadas pelo tamoxifeno.To report tamoxifen ocular toxicity. The best visual acuity was measured in both eyes with Snellen chart, slit-lamp examination of anterior segment, refraction, dilated fundus examination, fluorescein angiography and retinography in a 63-year-old patient, female, white, using tamoxifen 20 mg/day for 4 years, with 20/70 and 20/40 corrected visual acuity. The anterior segment examination showed corneal linear subepithelial opacity inferior to the visual axis and nuclear and posterior cortical cataract (1+/4 in both eyes. Fundus examination showed alteration of macular color in both eyes. Fluorescein angiography presented hyperfluorescence in the macular area at an early phase (window defect. Report of keratopathy and maculopathy caused by tamoxifen.

  15. Anti-Candida activity of Quercus infectoria gall extracts against Candida species

    Directory of Open Access Journals (Sweden)

    Nur Saeida Baharuddin

    2015-01-01

    Full Text Available Background: Galls of Quercus infectoria have been traditionally used to treat common ailments, including yeast infections caused by Candida species. Objective: This study aimed to evaluate the in vitro anti-Candida activity of Q. infectoria gall extracts against selected Candida species. Materials and Methods: Methanol and aqueous extracts of Q. infectoria galls were tested for anti-Candida activity against Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis. The minimum inhibitory concentrations were determined using the two-fold serial dilution technique of concentrations ranging from 16 mg/ml to 0.03 mg/ml. After 24 h, the minimum fungicidal concentrations were determined by subculturing the wells, which showed no turbidity on the agar plate. Potential phytochemical group in the crude extracts was screened by phytochemical qualitative tests and subsequently subjected to the gas chromatography-mass spectrometry analysis. Results: Both methanol and aqueous extracts displayed substantial anti-Candida activity and pyrogallol was the major component of both crude extracts. Conclusions: Data from current study suggested that Q. infectoria gall extracts are a potential source to be developed as anti-candidiasis.

  16. Performance of chromogenic media for Candida in rapid presumptive identification of Candida species from clinical materials

    Directory of Open Access Journals (Sweden)

    M V Pravin Charles

    2015-01-01

    Full Text Available Background: In perspective of the worldwide increase in a number of immunocompromised patients, the need for identification of Candida species has become a major concern. The development of chromogenic differential media, introduced recently, facilitate rapid speciation. However, it can be employed for routine mycology workup only after an exhaustive evaluation of its benefit and cost effectiveness. This study was undertaken to evaluate the benefit and cost effectiveness of chromogenic media for speciation of Candida clinical isolates. Materials and Methods: Sputum samples of 382 patients were screened for the presence of Candida spp. by Gram stain and culture on sabouraud dextrose agar. Candida species were identified using Gram stain morphology, germ tube formation, cornmeal agar with Tween-80, sugar fermentation tests and morphology on HiCrome Candida differential agar. All the Candida isolates were inoculated on HiCrome Candida agar (HiMedia, Mumbai, India. Results: The sensitivity and specificity of HiCrome agar for identification of Candida albicans were 90% and 96.42%, respectively whereas sensitivity and specificity of carbohydrate fermentation test were 86.67% and 74.07%, respectively. Sensitivity and specificity values of HiCrome agar for detection of C. albicans, Candida parapsilosis and Candida glabrata were above 90%. Conclusions: We found HiCrome agar has high sensitivity and specificity comparable to that of the conventional method. In addition, use of this differential media could significantly cut down the turnaround time as well as cost of sample processing.

  17. Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate

    Directory of Open Access Journals (Sweden)

    Apurva K Pathak

    2012-02-01

    Full Text Available OBJECTIVE: In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. MATERIAL AND METHODS: The species of Candida, isolated from multiple species oral candidiasis of the neutropenic patients, were used for the experiment. Organisms were cultured on Sabouraud dextrose broth with 8% glucose (SDB. Biofilm production on the acrylic resins strips was determined by crystal violet assay. Student's t-test and ANOVA were used to compare in vitro biofilm formation for the individual species of Candida and its different multi-species combinations. RESULTS: In the present study, differences between the mean values of the biofilm-forming ability of individual species (C. glabrata>C. krusei>C. tropicalis>C. albicans and in its multi-species' combinations (the highest for C. albicans with C. glabrata and the lowest for all the four species combination were reported. CONCLUSIONS: The findings of this study showed that biofilm-forming ability was found greater for non-Candida albicans Candida species (NCAC than for C. albicans species with intra-species variation. Presence of C. albicans in multi-species biofilms increased, whereas; C. tropicalis decreased the biofilm production with all other NCAC species.

  18. Definición de estrategias de operación en procesos biotecnológicos mediante el uso de técnicas de monitorización y modelización. Aplicación a la producción de lipasas por Candida rugosa

    OpenAIRE

    Montesinos Seguí, José Luis

    1993-01-01

    En aquest treball es presenten el resultats obtinguts en l'estudi de la producció de lipases per Candida rugosa amb l'objectiu de definir estratègies d'operació que millorin la productivitat. A més s'han desenvolupat metodologies de treball que poden ser aplicables a un ampli ventall de processos biotecnològics.La selecció del medi de cultiu i mode d'operació adients han permès millorar en 25 vegades el rendiment lipasa/substrat original. Aquesta millora ha estat possible gràcies a la definic...

  19. Estudio de quitridiomicosis por Batrachochytrium dendrobatidis en anfibios anuros del Litoral, Cuyo y Patagonia Argentina

    OpenAIRE

    Ghirardi, Romina

    2012-01-01

    La quitridiomicosis es una enfermedad emergente que en los últimos años ha sido vinculada a la muerte masiva de los anfibios. Esta enfermedad es causada por el hongo acuático zoospórico Batrachochytrium dendrobatidis -Bd- (Orden Rhyzophydiales). La infección de los anfibios comienza cuando las zoosporas móviles contactan un animal susceptible y penetran en su piel. Las alteraciones producidas por esta infección interfieren con varias funciones epiteliales de los anfibios como la circulación y...

  20. Priones y encefalopatías espongiformes transmisibles: un recorrido por su historia

    OpenAIRE

    Pena, Irene

    2011-01-01

    Las enfermedades causadas por priones son un grupo de enfermedades neurodegenerativas, producidas por el metabolismo aberrante de una proteína, que afectan tanto a los animales como a la especie humana. Presentan un prolongado período de incubación, alta transmisibilidad y una evolución clínica fatal y careceden de tratamiento. Se ha utilizado el término de prionpatías para denominarlas y debido a la espongiosis que producen en el sistema nervioso también se les conoce con el nombre de encefa...

  1. Estudio del daño en el ADN inducido por compuestos polifenólicos de la dieta

    OpenAIRE

    Burgos Morón, Estefanía

    2015-01-01

    El cáncer es una enfermedad causada por la acumulación de daño en el ADN . Las células cancerosas presentan numerosas alteraciones genéticas, epigenéticas y cromosómicas, que juegan un papel fundamental en el desarrollo de la enfermedad . Estas alteraciones pueden producirse como consecuencia del propio funcionamiento celular. Por ejemplo, se sabe que las especies reactivas de oxígeno generadas durante la respiración celular y los errores cometidos por las enzimas ADN polimerasas durante la r...

  2. Metal Ions May Suppress or Enhance Cellular Differentiation in Candida albicans and Candida tropicalis Biofilms▿ †

    OpenAIRE

    Harrison, Joe J.; Ceri, Howard; Yerly, Jerome; Rabiei, Maryam; Hu, Yaoping; Martinuzzi, Robert; Turner, Raymond J.

    2007-01-01

    Candida albicans and Candida tropicalis are polymorphic fungi that develop antimicrobial-resistant biofilm communities that are characterized by multiple cell morphotypes. This study investigated cell type interconversion and drug and metal resistance as well as community organization in biofilms of these microorganisms that were exposed to metal ions. To study this, Candida biofilms were grown either in microtiter plates containing gradient arrays of metal ions or in the Calgary Biofilm Devi...

  3. Isolated Candida infection of the lung

    Directory of Open Access Journals (Sweden)

    Yousef Shweihat

    2015-01-01

    Full Text Available Candida pneumonia is a rare infection of the lungs, with the majority of cases occurring secondary to hematological dissemination of Candida organisms from a distant site, usually the gastrointestinal tract or skin. We report a case of a 77-year-old male who is life-long smoker with a history of rheumatoid arthritis and polymyalgia rheumatica, but did not take immunosuppressants for those conditions. Here, we present an extremely rare case of isolated pulmonary parenchymal Candida infection in the form pulmonary nodules without evidence of systemic disease which has only been described in a few previous reports.

  4. Candida albicans osteomyelitis of the cervical spine

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Jang-Gyu; Hong, Hyun-Sook [Soonchunhyang University Bucheon Hospital, Department of Radiology, Bucheon-Si, Gyeonggi-Do (Korea); Koh, Yoon-Woo [Soonchunhyang University Bucheon Hospital, Department of Otolaryngology - Head and Neck Surgery, Bucheon-Si, Gyeonggi-Do (Korea); Kim, Hee-Kyung [Soonchunhyang University Bucheon Hospital, Department of Pathology, Bucheon-Si, Gyeonggi-Do (Korea); Park, Jung-Mi [Soonchunhyang University Bucheon Hospital, Department of Nuclear Medicine, Bucheon-Si, Gyeonggi-Do (Korea)

    2008-04-15

    Fungal osteomyelitis is a rare infection that usually develops in immunocompromised patients. Additionally, involvement of the cervical spine by Candida albicans is extremely rare; only three previous cases of Candida vertebral osteomyelitis have been reported in the literature. The diagnosis may be delayed due to nonspecific radiologic findings and a slow progression. We report the CT, MRI, bone scan, and PET-CT findings in a patient who developed Candida osteomyelitis, which was initially misdiagnosed as metastasis, at the atlas and axis following treatment for nasopharyngeal cancer. (orig.)

  5. Candida albicans osteomyelitis of the cervical spine

    International Nuclear Information System (INIS)

    Fungal osteomyelitis is a rare infection that usually develops in immunocompromised patients. Additionally, involvement of the cervical spine by Candida albicans is extremely rare; only three previous cases of Candida vertebral osteomyelitis have been reported in the literature. The diagnosis may be delayed due to nonspecific radiologic findings and a slow progression. We report the CT, MRI, bone scan, and PET-CT findings in a patient who developed Candida osteomyelitis, which was initially misdiagnosed as metastasis, at the atlas and axis following treatment for nasopharyngeal cancer. (orig.)

  6. Biotechnological production of xylitol with Candida yeasts

    OpenAIRE

    Granström, Tom

    2002-01-01

    The aim of this study was to develop a biotechnological production process for xylitol. The xylitol production characteristics of Candida millerii, Candida guilliermondii and Candida tropicalis were compared. C. tropicalis was the best xylitol producer. A volumetric productivity of 5.7 g xylitol L-1 h-1 was achieved with 69 % yield from D-xylose on a mineral medium with a modified repeated fed batch production method. The xylitol production mechanism was confirmed by chemostat cultivation stu...

  7. Compactação causada pelo manejo de plantas invasoras em latossolo vermelho-amarelo cultivado com cafeeiros

    OpenAIRE

    Paula Sant'Anna Moreira Pais; Moacir de Souza Dias Junior; Gislene Aparecida dos Santos; Adriana Cristina Dias; Paulo Tácito Gontijo Guimarães; Elifas Nunes Alcântara

    2011-01-01

    O manejo de plantas invasoras é considerado uma das principais atividades que promovem degradação da estrutura do solo em lavouras cafeeiras, devido à compactação do solo causada pelas operações de controle de plantas invasoras. O objetivo deste estudo foi determinar o modelo de capacidade de suporte de carga para o manejo de plantas invasoras sem capina, bem como, utilizando esse modelo, qual manejo de plantas invasoras causa menor ou maior compactação do solo. Este estudo foi realizado em u...

  8. Antibiofilm activity of carboxymethyl chitosan on the biofilms of non-Candida albicans Candida species.

    Science.gov (United States)

    Tan, Yulong; Leonhard, Matthias; Moser, Doris; Schneider-Stickler, Berit

    2016-09-20

    Although most cases of candidiasis have been attributed to Candida albicans, non-C. albicans Candida species have been isolated in increasing numbers in patients. In this study, we determined the inhibition of carboxymethyl chitosan (CM-chitosan) on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata. Biofilm by all tested species in microtiter plates were inhibited nearly 70%. CM-chitosan inhibited mixed species biofilm in microtiter plates and also on medical materials surfaces. To investigate the mechanism, the effect of CM-chitosan on cell viability and biofilm growth was employed. CM-chitosan inhibited Candida planktonic growth as well as adhesion. Further biofilm formation was inhibited with CM-chitosan added at 90min, 12h or 24h after biofilm initiation. CM-chitosan was not only able to inhibit the metabolic activity of Candida cells, but was also active upon the establishment and the development of biofilms. PMID:27261732

  9. Candida glabrata among Candida spp. from environmental health practitioners of a Brazilian Hospital

    OpenAIRE

    Catarina Savastano; Elisa de Oliveira Silva; Lindyanne Lemos Gonçalves; Jéssica Maria Nery; Naiara Chaves Silva; Amanda Latercia Tranches Dias

    2016-01-01

    Abstract The incidence of the species Candida albicans and non-albicans Candida was evaluated in a Brazilian Tertiary Hospital from the environment and health practitioners. In a 12-month period we had a total positivity of 19.65% of Candida spp. The most recurring non-albicans Candida species was C. glabrata (37.62%), generally considered a species of low virulence, but with a higher mortality rate than C. albicans. Subsequently, C. parapsilosis (25.74%) and C. tropicalis (16.86%) were the s...

  10. Atividade antifúngica do extrato aquoso de Arctium minus (Hill) Bernh. (Asteraceae) sobre espécies orais de Candida Antifungal activity of the aqueous extract from Arctium minus (Hill) Bernh. (Asteraceae) on oral Candida species

    OpenAIRE

    C.T Lubian; J.M. Teixeira; R.G Lund; P.S. Nascente; F.A.B Del Pino

    2010-01-01

    Este estudo teve como objetivo avaliar a atividade antifúngica do extrato aquoso de folhas de "bardana" [Arctium minus (Hill) Bernh.], por meio da determinação da Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) sobre diferentes espécies e linhagens do gênero Candida. Foram incluídas no trabalho as espécies de Candida albicans, C. tropicalis, C. glabrata, C. stellatoidea, C. dubliniensis e C. krusei. Utilizaram-se quatro linhagens de C. albicans e três de C.tropicali...

  11. Skin Immunity to Candida albicans.

    Science.gov (United States)

    Kashem, Sakeen W; Kaplan, Daniel H

    2016-07-01

    Candida albicans is a dimorphic commensal fungus that colonizes healthy human skin, mucosa, and the reproductive tract. C. albicans is also a predominantly opportunistic fungal pathogen, leading to disease manifestations such as disseminated candidiasis and chronic mucocutaneous candidiasis (CMC). The differing host susceptibilities for the sites of C. albicans infection have revealed tissue compartmentalization with tailoring of immune responses based on the site of infection. Furthermore, extensive studies of host genetics in rare cases of CMC have identified conserved genetic pathways involved in immune recognition and the response to the extracellular pathogen. We focus here on human and mouse skin as a site of C. albicans infection, and we review established and newly discovered insights into the cellular pathways that promote cutaneous antifungal immunity. PMID:27178391

  12. Xylitol production by Candida parapsilosis under fed-batch culture

    Directory of Open Access Journals (Sweden)

    Sandra A. Furlan

    2001-06-01

    Full Text Available Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose or mixed (xylose and glucose sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.A produção de xylitol por Candida parapsilosis foi investigada em regime de batelada alimentada, usando substratos açucarados de composição simples (xilose ou composta (xilose e glicose. A presença de glicose no meio induziu a formação de etanol como metabólito secundário. A suplementação fracionada do meio de alimentação numa concentração fixa de açúcar não resultou em aumento da produtividade em relação àquela alcançada em batelada simples.

  13. Development of DNA probes for Candida albicans

    International Nuclear Information System (INIS)

    An attempt was made to produce DNA probes that could be used as a rapid and efficient means of detecting candidiasis (invasive Candida infection) in immunocompromised patients. Whole DNA from Candida albicans was digested with restriction endonuclease, and the resulting fragments were randomly cloned into a plasmid vector. Several recombinant plasmids were evaluated for cross-hybridization to various other Candida species, other fungal DNAs, and to nonfungal DNAs. Cross reactions were observed between the probes and different yeasts, but none with unrelated DNAs. Some recombinants were genus-specific, and two of these were applied to the analysis of C. albicans growth curves. It became evident that, although both 32P- and biotin-labelled probes could be made quite sensitive, a possible limitation in their diagnostic potential was the poor liberation of Candida DNA from cells. Thus, better methods of treatment of clinical specimens will be required before such probes will be useful in routine diagnosis

  14. Candida infection of a prosthetic shoulder joint

    International Nuclear Information System (INIS)

    A heroin addict developed a Candida parapsilosis infection in a prosthetic shoulder joint. Radiographs showed loose fragments of cement with prosthetic loosening. The patient was treated with removal of the prosthesis and intravenous amphotericin B followed by oral ketoconazole. (orig.)

  15. Development of DNA probes for Candida albicans

    Energy Technology Data Exchange (ETDEWEB)

    Cheung, L.L.; Hudson, J.B.

    1988-07-01

    An attempt was made to produce DNA probes that could be used as a rapid and efficient means of detecting candidiasis (invasive Candida infection) in immunocompromised patients. Whole DNA from Candida albicans was digested with restriction endonuclease, and the resulting fragments were randomly cloned into a plasmid vector. Several recombinant plasmids were evaluated for cross-hybridization to various other Candida species, other fungal DNAs, and to nonfungal DNAs. Cross reactions were observed between the probes and different yeasts, but none with unrelated DNAs. Some recombinants were genus-specific, and two of these were applied to the analysis of C. albicans growth curves. It became evident that, although both /sup 32/P- and biotin-labelled probes could be made quite sensitive, a possible limitation in their diagnostic potential was the poor liberation of Candida DNA from cells. Thus, better methods of treatment of clinical specimens will be required before such probes will be useful in routine diagnosis.

  16. Sequence resources at the Candida Genome Database

    OpenAIRE

    Arnaud, Martha B.; Costanzo, Maria C.; Skrzypek, Marek S.; Shah, Prachi; Binkley, Gail; Lane, Christopher; Miyasato, Stuart R.; SHERLOCK, Gavin

    2006-01-01

    The Candida Genome Database (CGD, ) contains a curated collection of genomic information and community resources for researchers who are interested in the molecular biology of the opportunistic pathogen Candida albicans. With the recent release of a new assembly of the C.albicans genome, Assembly 20, C.albicans genomics has entered a new era. Although the C.albicans genome assembly continues to undergo refinement, multiple assemblies and gene nomenclatures will remain in widespread use by the...

  17. Candida Sepsis Following Transcervical Chorionic Villi Sampling

    OpenAIRE

    Israel Potasman; Roni Gonen; Alona Paz

    2001-01-01

    Background: The use of invasive devices and broad spectrum antibiotics has increased the rate of candidal superinfections.Candida sepsis associated with pregnancy is rare. Candida sepsis following chorionic villi sampling (CVS) has never been reported. Case: A 31-year-old pregnant woman presented with signs of sepsis one day after undergoing transcervical CVS. Blood culture and curettage material yielded C. albicans. She was treated with 400 mg of fluconazole daily for 4 weeks and completely ...

  18. Oral Candida infections--a review.

    OpenAIRE

    Samaranayake L; Nair R

    1995-01-01

    Candida species are the commonest agents of oral mycoses. They cause a variety of diseases including the new variant, erythematous candidosis, which is frequently described in HIV infection. Due to these and other reasons the classification of oral candidosis has been recently revised, and further more new therapeutic regimes have been described. Hence in this article an overview of oral Candida infections is presented with special emphasis on current concepts related to classification and tr...

  19. Characterization of Mucosal Candida albicans Biofilms

    OpenAIRE

    Dongari-Bagtzoglou, Anna; Kashleva, Helena; Dwivedi, Prabhat; Diaz, Patricia; Vasilakos, John

    2009-01-01

    C. albicans triggers recurrent infections of the alimentary tract mucosa that result from biofilm growth. Although the ability of C. albicans to form a biofilm on abiotic surfaces has been well documented in recent years, no information exists on biofilms that form directly on mucosal surfaces. The objectives of this study were to characterize the structure and composition of Candida biofilms forming on the oral mucosa. We found that oral Candida biofilms consist of yeast, hyphae, and commens...

  20. Candida species isolated from various clinical specimens

    OpenAIRE

    Uraz, Güven; Arslan, Seza; Ekener, Serpil

    2000-01-01

    Objectives: The various culture specimens of patients were investigated in our research. Material and methods: 123 Candida strains were isolated. In identification of Candida species that were isolated, germ tube test, growth in Cornmeal-Tween 80 agar and formation of clamydospore, presence of pseudohyphae, carbonhytrate fermentation and assimilation tests, formation of membranes in sabouraud medium, and the tests of ascospore in the mediums with cycloheximide and the test of nitrate were...

  1. Molecular screening for Candida orthopsilosis and Candida metapsilosis among Danish Candida parapsilosis group blood culture isolates: proposal of a new RFLP profile for differentiation

    DEFF Research Database (Denmark)

    Mirhendi, Hossein; Bruun, Brita; Schønheyder, Henrik Carl;

    2010-01-01

    Candida orthopsilosis and Candida metapsilosis are recently described species phenotypically indistinguishable from Candida parapsilosis . We evaluated phenotyping and molecular methods for the detection of these species among 79 unique blood culture isolates of the C. parapsilosis group obtained...... number of invasive infections in Denmark....

  2. PET-CT manifestation of Candida esophagitis

    Energy Technology Data Exchange (ETDEWEB)

    Bahk, Yong Whee [Sung-Ae Hospital, Seoul (Korea, Republic of); O, Joo Hyun [Kangnam St. Mary' s Hospital, Catholic University Medical School, Seoul (Korea, Republic of)

    2007-04-15

    Candida esophagitis (moniliasis) is the most common infection of the gullet and has generally been attributed to as a complication of immune suppressed state. However, as the current case. Holt found the disease to occur in 3 of his 13 patients without predisposing condition. Predisposing factors other than immune deficient conditions include aplastic anemia, alcoholism and Parkinson's disease and age, diabetes mellitus, and disruption of mucosal integrity. Growing prevalence of Candida esophagitis in recent years is accounted for by an increase in the number of patients with organ transplantation, malignancy and AIDS as well as populrization of endoscopy. Microorganisms that reached the esophagus in oral secretions are rarely cultured from the esophageal surface. Of many species C. albicans is the most common offender although C. tropicalis has also been isolated with high prevalence, particularly in the patients with cancer and disseminated candidiasis. Clinically, the patients with Candida esophagitis seek medical care for esophageal or retrosternal pain, dysphagia or distress. Candida esophagitis may be the extension from oropharyngeal infection but in the majority the esophagus is the sole site of infection. The middle and lower thirds of the esophagus are more typically affected than the upper third. Diagnosis can be indicated by double contrast esophagography or endoscopy and confirmed by potassium hydroxide (KOH) stain or biopsy. It is to be noted that the more presence of Candida in smear or cultured specimen cannot indict Candida as definitive offender. Differential diagnosis includes herpes simplex infection, cytomegalovirus infection, reflux esophagitis or radiation esophagitis.

  3. PET-CT manifestation of Candida esophagitis

    International Nuclear Information System (INIS)

    Candida esophagitis (moniliasis) is the most common infection of the gullet and has generally been attributed to as a complication of immune suppressed state. However, as the current case. Holt found the disease to occur in 3 of his 13 patients without predisposing condition. Predisposing factors other than immune deficient conditions include aplastic anemia, alcoholism and Parkinson's disease and age, diabetes mellitus, and disruption of mucosal integrity. Growing prevalence of Candida esophagitis in recent years is accounted for by an increase in the number of patients with organ transplantation, malignancy and AIDS as well as populrization of endoscopy. Microorganisms that reached the esophagus in oral secretions are rarely cultured from the esophageal surface. Of many species C. albicans is the most common offender although C. tropicalis has also been isolated with high prevalence, particularly in the patients with cancer and disseminated candidiasis. Clinically, the patients with Candida esophagitis seek medical care for esophageal or retrosternal pain, dysphagia or distress. Candida esophagitis may be the extension from oropharyngeal infection but in the majority the esophagus is the sole site of infection. The middle and lower thirds of the esophagus are more typically affected than the upper third. Diagnosis can be indicated by double contrast esophagography or endoscopy and confirmed by potassium hydroxide (KOH) stain or biopsy. It is to be noted that the more presence of Candida in smear or cultured specimen cannot indict Candida as definitive offender. Differential diagnosis includes herpes simplex infection, cytomegalovirus infection, reflux esophagitis or radiation esophagitis

  4. Multilocus sequence typing confirms synonymy but highlights differences between Candida albicans and Candida stellatoidea.

    NARCIS (Netherlands)

    Jacobsen, M.D.; Boekhout, T.; Odds, F.C.

    2008-01-01

    We used multi-locus sequence typing (MLST) to investigate 35 yeast isolates representing the two genome-sequenced strains plus the type strain of Candida albicans, four isolates originally identified as Candida stellatoidea type I and 28 representing type strains of other species now regarded as syn

  5. Identificación y cuantificación de los efectos y consecuencias de los impactos ambientales generados por la mineria de extracción de oro por lixiviación de cianuro. Caso Mineria Yanacocha SRL, en Cajamarca, Perú

    OpenAIRE

    Yacoub López, Cristina

    2007-01-01

    En el proyecto realizado se ha estudiado la situación de vulnerabilidad medioambiental, específicamente del recurso hídrico, causada por la empresa Minera Yanacocha S.R.L (MYSRL) situada en la región de Cajamarca, en el Perú. La empresa minera es origen de conflictos en relación al agua, por su situación (cabecera de cuatro cuencas hidrográficas) y por la contaminación en la cantidad y calidad de sus aguas percibida por la población local. Para conocer los posibles impactos del...

  6. Choque séptico por ehrliquiosis

    Directory of Open Access Journals (Sweden)

    Viviana Hernández-de Mezerville

    2007-04-01

    Full Text Available La ehrliquiosis humana es una infección zoonótica transmitida por "garrapatas duras" causada por Anaplasma sp y Ehrlichia sp. Puede presentarse como un cuadro pseudogripal con fiebre, malestar general, mialgias y cefalea o hasta un choque séptico con falla multiorgánica y muerte. La mitad de los pacientes requieren atención hospitalaria en algún momento y se reporta una mortalidad de hasta el 5% secundaria a enfermedades oportunistas o a hemorragias. El diagnóstico se puede sospechar visualizando las mórulas intracitoplasmáticas en las células afectadas en frotis de sangre periférica, y se confirma con serología, con cultivos o con detección molecular. El tratamiento de elección es la doxiciclina con buena respuesta clínica. Se reporta un caso probable de ehrliquiosis humana asociado a choque séptico y falla multiorgánica tratado exitosamente en el Hospital Dr. Rafael Angel Calderón Guardia.

  7. por valores

    Directory of Open Access Journals (Sweden)

    Jazmín Díaz-Barrios

    2005-01-01

    Full Text Available La era actual se caracteriza por una búsqueda de la identidad del hombre, donde el pivote del cambio organizacional es el individuo; cambiar en este entorno depende, en gran medida, de los valores que imperen en la cultura de la organización. Este trabajo documental intenta identificar aquellos valores, condición sine-qua-non, para el logro de los objetivos de cambio y analizarlos a fin de determinar sus indicadores, con el objeto de presentar una herramienta a las organizaciones que les facilite adaptarse a los nuevos tiempos. Se encontró que los valores: Delegación, Comunicación, Colaboración, Participación y Aprendizaje, son esenciales en los cambios integrales de esta era. Se determinaron 20 indicadores de su presencia. Se concluye que con esta información cada ente puede elaborar instrumentos que le permitan saber si los valores requeridos están presentes y en caso contrario tomar decisiones que alineen a la organización alrededor de los valores humanistas planteados, incrementando así las posibilidades de éxito en el proceso de cambio y por ende, las posibilidades de supervivencia en esta nueva época.

  8. Description of an injury in a human caused by a false tocandira (Dinoponera gigantea, Perty, 1833 with a revision on folkloric, pharmacological and clinical aspects of the giant ants of the genera Paraponera and Dinoponera (sub-family Ponerinae Descrição de injúria humana causada por falsa tocandira (Dinoponera gigantea, Perty, 1833 com revisão dos aspectos folclóricos, farmacológicos e clínicos das formigas gigantes do gênero Paraponera e Dinoponera (sub-família Ponerinae

    Directory of Open Access Journals (Sweden)

    Vidal Haddad Junior

    2005-08-01

    Full Text Available The authors observed an injury caused by the sting of a false tocandira ant in the hand of an amateur fisherman and they describe the clinical findings and the evolution of the envenoming, which presented an acute and violent pain, cold sweating, nausea, a vomiting episode, malaise, tachycardia and left axillary's lymphadenopathy. About three hours after the accident, still feeling intense pain in the place of the sting, he presented an episode of great amount of blood in the feces with no history of digestive, hematological or vascular problems. The intense pain decreased after eight hours, but the place stayed moderately painful for about 24 hours. In that moment, he presented small grade of local edema and erythema. The authors still present the folkloric, pharmacological and clinical aspects related to the tocandiras stings, a very interesting family of ants, which presents the largest and more venomous ants of the world.A partir de um acidente causado pela picada de uma formiga falsa tocandira na mão de um pescador amador, os autores descrevem os achados clínicos locais observados, tais como edema, eritema e dor excruciante e a evolução do envenenamento, que cursou com fenômenos sistêmicos imediatos, como sudorese fria, náuseas, vômitos, mal estar, taquicardia e linfadenopatia axilar à esquerda. Após três horas, a dor intensa persistia e o paciente apresentou um episódio de hematoquesia, sem história anterior de enfermidades do trato digestivo, hematológicas ou vasculares. O uso de analgésicos (Tramal® 300 mg/dia, água quente e gelo não melhorou a dor, que arrefeceu em oito horas, tendo permanecido por cerca de 24 horas. São apresentados ainda os aspectos folclóricos, farmacológicos e clínicos relacionados às picadas de tocandiras.

  9. Levels of (1→3-β-D-glucan, Candida mannan and Candida DNA in serum samples of pediatric cancer patients colonized with Candida species

    Directory of Open Access Journals (Sweden)

    Khan Zia U

    2010-10-01

    Full Text Available Abstract Background Surveillance cultures may be helpful in identifying patients at increased risk of developing invasive candidiasis. However, only scant information exists on the effect of Candida colonization on serum levels of diagnostic biomarkers. This prospective surveillance study determined the extent of Candida colonization among pediatric cancer patients and its possible impact on serum levels of (1-3-β-D-glucan (BDG, Candida mannan and Candida DNA. Methods A total of 1075 swabs originating from oropharynx (n = 294, nostrils (n = 600, rectum (n = 28, groin (n = 50, ear (n = 54, and axilla (n = 49 of 63 pediatric cancer patients were cultured for the isolation of Candida spp. Patients yielding Candida spp. from any sites were considered as colonized. Serum samples were collected from patients at the time of first surveillance culture for detection of BDG by Fungitell kit and Candida mannan by Platelia Candida Ag. Candida DNA was detected by using panfungal primers and identification was carried out by using species-specific primers and DNA sequencing. Results Seventy-five (7.6% swab cultures from 35 (55.5% patients yielded Candida spp. These isolates included C. albicans (n = 62, C. dubliniensis (n = 8, C. glabrata and C. tropicalis (n = 2 each and C. krusei (n = 1. Eleven patients were colonized at three or more sites. Eight of 36 serum samples from 6 colonized patients yielded BDG values higher than the currently recommended cut-off value of ≥80 pg/ml. However, none of the serum samples yielded Candida mannan levels ≥0.5 ng/ml and PCR test for Candida DNA was also negative in all the serum samples of colonized patients. During the study period, only two colonized patients subsequently developed candidemia due to C. tropicalis. Besides positive blood cultures, C. tropicalis DNA, BDG and Candida mannan were also detected in serum samples of both the patients. Conclusions The present study demonstrates that while mucosal

  10. Isolation and Identification of Candida from the Oral Cavity

    OpenAIRE

    Shashanka Rajappa; Smitha Byadarahally Raju

    2011-01-01

    Various techniques are available for the isolation of Candida within the oral cavity. Such methods play an important role in the diagnosis and management of oral candidosis. The growing importance of Candida is in part related to the emergence of HIV infection and the more widespread use of immunosuppressive chemotherapy. Along with the Candida albicans there has been a greater recognition of the importance of the nonalbicans Candida species in oral candidosis. Identification of infecting str...

  11. Non-albicans Candida Infection: An Emerging Threat

    OpenAIRE

    Deorukhkar, Sachin C.; Santosh Saini; Stephen Mathew

    2014-01-01

    The very nature of infectious diseases has undergone profound changes in the past few decades. Fungi once considered as nonpathogenic or less virulent are now recognized as a primary cause of morbidity and mortality in immunocompromised and severely ill patients. Candida spp. are among the most common fungal pathogens. Candida albicans was the predominant cause of candidiasis. However, a shift toward non-albicans Candida species has been recently observed. These non-albicans Candida species d...

  12. The Host’s Reply to Candida Biofilm

    OpenAIRE

    Nett, Jeniel E.

    2016-01-01

    Candida spp. are among the most common nosocomial fungal pathogens and are notorious for their propensity toward biofilm formation. When growing on a medical device or mucosal surface, these organisms reside as communities embedded in a protective matrix, resisting host defenses. The host responds to Candida biofilm by depositing a variety of proteins that become incorporated into the biofilm matrix. Compared to free-floating Candida, leukocytes are less effective against Candida within a bio...

  13. Screening of Tanzanian medicinal plants for anti-Candida activity

    OpenAIRE

    Joseph Cosam C; Ngassapa Olipa D; Matee Mecky IN; Runyoro Deborah KB; Mbwambo Zakaria H

    2006-01-01

    Abstract Background Candida albicans has become resistant to the already limited, toxic and expensive anti-Candida agents available in the market. These factors necessitate the search for new anti-fungal agents. Methods Sixty-three plant extracts, from 56 Tanzanian plant species obtained through the literature and interviews with traditional healers, were evaluated for anti-Candida activity. Aqueous methanolic extracts were screened for anti-Candida activity by bioautography agar overlay meth...

  14. Comparison Between Virulence Factors of Candida albicans and Non-Albicans Species of Candida Isolated from Genitourinary Tract

    OpenAIRE

    Udayalaxmi,; Jacob, Shani; D’Souza, Diney

    2014-01-01

    Background: Candida spp. is frequently isolated from cases of vulvovaginal candidiasis and catheter associated UTI. C.albicans is the most frequently isolated species but non-albicans species of candida are gaining clinical significance.

  15. Severe Candida spp. infections: new insights into natural immunity.

    NARCIS (Netherlands)

    Meer, J.W.M. van der; Veerdonk, F.L. van de; Joosten, L.A.B.; Kullberg, B.J.; Netea, M.G.

    2010-01-01

    Invasive infections caused by Candida spp. are associated with high mortality. Colonisation by Candida spp. and the capacity of the host to recognise them as potential pathogens are essential steps in the development of these infections. The major pathogen-associated molecular patterns of Candida ar

  16. Treinamento resistido controla a pressão arterial de ratos hipertensos induzidos por l-NAME

    OpenAIRE

    Ayslan Jorge Santos de Araujo; Anne Carolline Veríssimo dos Santos; Karine dos Santos Souza; Marlúcia Bastos Aires; Valter Joviniano Santana-Filho; Emerson Ticona Fioretto; Marcelo Mendonça Mota; Márcio Roberto Viana Santos

    2013-01-01

    FUNDAMENTO: A hipertensão arterial é uma síndrome multifatorial, crônica, causada tanto por fatores congênitos ou adquiridos. OBJETIVO: Avaliar os efeitos do treinamento físico resistido (TR) sobre pressão arterial, reatividade e morfologia vascular de ratos hipertensos induzidos por L-NAME. MÉTODOS: Ratos Wistar machos (200-250 g) foram divididos em 3 grupos: normotenso sedentário (NS), hipertenso sedentário (HS) e hipertenso treinado (HT). A hipertensão foi induzida pela administração de L-...

  17. Optimização da detecção de Pneumocystis jirovecii por citometria de fluxo

    OpenAIRE

    Bragada, Cláudia Sofia Pássaro

    2008-01-01

    A pneumonia por Pneumocystis (PCP) em humanos é causada por um fungo actualmente denominado Pneumocystis jirovecii, anteriormente conhecido como Pneumocystis carinii. Trata-se da infecção pulmonar oportunista mais prevalente em doentes imunodeprimidos infectados com o vírus da imunodeficiência adquirida, com patologias hematológicas de natureza oncológica, doenças inflamatórias, deficiências primárias da imunidade celular ou sob terapêuticas imunossupressoras (como transplantados de órgãos só...

  18. Infestación por garrapatas en el ganado criollo lechero tropical en Veracruz, México

    OpenAIRE

    González Cerón, Fernando

    2012-01-01

    El presente estudio tuvo como objetivo caracterizar la infestación por garrapatas en un hato experimental de ganado Criollo Lechero Tropical (CLT) ubicado en la región central del estado de Veracruz, México; la información generada permitiría iniciar el registro sistemático de la infestación causada por éstos ectoparásitos para considerarla como una posible característica a ser incluida en un programa de mejora genética a mediano plazo. A 28 vaquillas (655±35 días de edad) y 16 toretes (741±8...

  19. Modelo de probabilidad para predecir una neumonía nosocomial por Staphylococcus aureus resistente a meticilina

    OpenAIRE

    Tejero García, Rocio

    2010-01-01

    La neumonía mosocomial, es una de las principales infecciones hospitalarias y la de mayor morbimortalidad. Causada por una gran variedad de microorganismos, aumentando cada vez más los microorganismos multirresistentes como principales causantes, entre ellos, Staphylococcus aureus meticilín resistente. Se pretende con este estudio identificar una serie de factores de riesgo, con los que crear un modelo de probabilidad y unos criterios pronósticos para predecir la neumonía mosocomial por Staph...

  20. Perfil clínico y parasitológico de la malaria por Plasmodium falciparum y Plasmodium vivax no complicada en Córdoba, Colombia

    OpenAIRE

    Angélica Knudson-Ospina; Ricardo Sánchez-Pedraza; Manuel Alberto Pérez-Mazorra; Liliana Jazmín Cortés-Cortés; Ángela Patricia Guerra-Vega; Rubén Santiago Nicholls-Orejuela

    2015-01-01

    Antecedentes. En Colombia existen pocos estudios que buscan encontrar diferencias clínicas y parasitológicas en la malaria causada por Plasmodium falciparum y Plasmodium vivax. Objetivo. Describir el perfil clínico y parasitológico de las malarias por Plasmodium falciparum y Plasmodium vivax no complicadas en Tierralta, Córdoba, Colombia. Materiales y métodos. Se evaluaron pacientes con paludismo no complicado por Plasmodium falciparum y Plasmodium vivax según los protocolos estandarizados po...

  1. How to Use the Candida Genome Database.

    Science.gov (United States)

    Skrzypek, Marek S; Binkley, Jonathan; Sherlock, Gavin

    2016-01-01

    Studying Candida biology requires access to genomic sequence data in conjunction with experimental information that provides functional context to genes and proteins. The Candida Genome Database (CGD) integrates functional information about Candida genes and their products with a set of analysis tools that facilitate searching for sets of genes and exploring their biological roles. This chapter describes how the various types of information available at CGD can be searched, retrieved, and analyzed. Starting with the guided tour of the CGD Home page and Locus Summary page, this unit shows how to navigate the various assemblies of the C. albicans genome, how to use Gene Ontology tools to make sense of large-scale data, and how to access the microarray data archived at CGD. PMID:26519061

  2. How to use the Candida Genome Database

    Science.gov (United States)

    Skrzypek, Marek S.; Binkley, Jonathan; Sherlock, Gavin

    2016-01-01

    Summary Studying Candida biology requires access to genomic sequence data in conjunction with experimental information that provides functional context to genes and proteins. The Candida Genome Database (CGD) integrates functional information about Candida genes and their products with a set of analysis tools that facilitate searching for sets of genes and exploring their biological roles. This chapter describes how the various types of information available at CGD can be searched, retrieved, and analyzed. Starting with the guided tour of the CGD Home page and Locus Summary page, this unit shows how to navigate the various assemblies of the C. albicans genome, how to use Gene Ontology tools to make sense of large-scale data, and how to access the microarray data archived at CGD. PMID:26519061

  3. Colonização e espécies de Candida em pacientes submetidos à radioterapia cervicofacial Candida colonization and species in patients submitted to head and neck radiotherapy

    Directory of Open Access Journals (Sweden)

    Paulo Rogério Ferreti Bonan

    2007-12-01

    Full Text Available Os objetivos deste estudo foram investigar a colonização por Candida associada à redução do fluxo salivar e identificar as espécies antes, durante e após a radioterapia em 20 pacientes portadores de carcinomas de células escamosas, comparando com dados obtidos em dois grupos-controle, um formado por 24 pacientes saudáveis e o outro, por nove pacientes submetidos à radioterapia por pelo menos um ano. A saliva total de todos os pacientes foi coletada pelo método não-estimulado. As unidades formadoras de colônia de Candida foram quantificadas e identificadas pelos testes bioquímicos, CHROMagar e Api 20 Aux. Os resultados evidenciaram, no grupo de estudo, redução progressiva do fluxo salivar entre a primeira e a terceira coleta (p = 0,001 e aumento da positividade de espécies, com predominância das espécies C. albicans e C. tropicalis, bem como de sua diversificação. Houve correlação significativa entre redução do fluxo salivar e colonização por Candida (p = 0,009. Após 12 meses, o fluxo salivar apresentavase baixo e houve maior incidência das espécies C. albicans e C. tropicalis. Em suma, os pacientes irradiados com doses tumoricidas em campos cervicofaciais apresentaram redução do fluxo salivar, aumento na colonização fúngica e diversificação das espécies durante e no final do tratamento.The aims of this study were to investigate Candida carriage associated with reduced salivary flow and to identify Candida species in 20 patients with squamous cell carcinoma before, during and after head and neck radiotherapy, comparing with 2 control groups, one compounded by 24 healthful patients and the other, by nine patients submitted to head and neck radiotherapy one year at least. Whole non stimulated saliva were collected from all patients. Candida unit form colonies were quantified and identified using biochemistry tests, CHROMagar and API 20 Aux. The results showed progressive reduction of salivary flow during

  4. La responsabilidad del Estado por la actividad del legislador

    Directory of Open Access Journals (Sweden)

    María Consuelo Alonso García

    2012-12-01

    Full Text Available Analizar el fenómeno de la responsabilidad del Estado causada por los hechos del legislador ha sido un trabajo desarrollado tanto por la doctrina y la jurisprudencia internacional a mediados del siglo xx. En Francia, por ejemplo, se presentó el primer antecedente jurisprudencial en el año de 1934. En España, su estudio empezó a gestionarse con la promulgación de la Constitución de 1978, presentándose el primer fallo judicial en el año de 1993. Para el caso colombiano, la Constitución de 1991 introdujo en su artículo 90 el fundamento constitucional de la responsabilidad patrimonial del Estado, sin que esto significase que la jurisprudencia del Consejo de Estado encontrase las bases de esta responsabilidad en diferentes disposiciones de la Constitución. Sin embargo, los primeros fallos relacionados con este título de imputación en Colombia fueron emitidos por el máximo tribunal de lo contencioso administrativo en 1998, y por la Corte Constitucional a través de la sentencia C-038 de 2006, providencia judicial que estableció algunas de las características que posee esta figura jurídica.

  5. Commensal Oral Candida in Asian Cohorts

    Institute of Scientific and Technical Information of China (English)

    Lakshman Samaranayake

    2009-01-01

    The oral carriage rate of Candida in healthy humans ranges from 40% to 60%. However for a prolonged period, the oral candidal prevalence in humans was documented essentially using data from studies in the West as their prevalence in inhabitants in different regions of the world, including Asia was not known. Yet, recent reports from a number of studies indicate the quality, quantity and prevalence of oral yeasts differ between Asia and other regions for reason that are still unclear. This mini review on such data from Asian studies on oral carriage of Candida provides another intriguing facet of the behavior of this ubiquitous yeast.

  6. Caspofungin Etest susceptibility testing of Candida species

    DEFF Research Database (Denmark)

    Arendrup, Maiken Cavling; Pfaller, Michael A; Rosenvinge, Flemming Schønning

    2012-01-01

    The purpose of this study was to evaluate the performance of caspofungin Etest and the recently revised CLSI breakpoints. A total of 497 blood isolates, of which 496 were wild-type isolates, were included. A total of 65/496 susceptible isolates (13.1%) were misclassified as intermediate (I) or re...... resistant (R). Such misclassifications were most commonly observed for Candida krusei (73.1%) and Candida glabrata (33.1%). The revised breakpoints cannot be safely adopted for these two species....

  7. The Host’s Reply to Candida Biofilm

    Directory of Open Access Journals (Sweden)

    Jeniel E. Nett

    2016-03-01

    Full Text Available Candida spp. are among the most common nosocomial fungal pathogens and are notorious for their propensity toward biofilm formation. When growing on a medical device or mucosal surface, these organisms reside as communities embedded in a protective matrix, resisting host defenses. The host responds to Candida biofilm by depositing a variety of proteins that become incorporated into the biofilm matrix. Compared to free-floating Candida, leukocytes are less effective against Candida within a biofilm. This review highlights recent advances describing the host’s response to Candida biofilms using ex vivo and in vivo models of mucosal and device-associated biofilm infections.

  8. Artritis séptica por streptococcus pneumoniae: reporte de un caso

    OpenAIRE

    Novoa, C.; López, R.E.; Gómez Balboa, P.; Blas Dobón, J.A.; Bonet, B.; Rodrigo Pérez, José Luís

    2015-01-01

    La artritis neumocócica es infrecuente en adultos, predomina en pacientes pediátricos. La neumonía y la bacteriemia son las manifestaciones más frecuentes, la afectación articular según series recientes tiene una prevalencia menor al 1% en sujetos menores de 50 años. Se presenta el caso de una paciente de 60 años con LES (Lupus Eritematoso Sistémico) la cual presentó monoartritis séptica de rodilla derecha causada por Streptococcus pneumoniae

  9. Susceptibility characterisation of Candida spp. to four essential oils.

    Science.gov (United States)

    Rath, C C; Mohapatra, S

    2015-02-01

    In the present investigation, anti-Candida activity of four essential oils i.e. Black cumin (Nigella sativa), Curry leaf (Murraya koienigii), Ajwain (Trachiyspirum ammi), and Betel leaf (Piper betel) were screened against four human pathogenic species of Candida viz. Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. The minimum inhibitory concentration (MIC) values of the oils ranged between 15.62 and 250 μl/ml while studied through tube dilution method. The oils retained their anti-Candida activities even after heat treatment (at 45ΊC, 60ΊC, 100ΊC for 1 hour) and also on autoclaving. Both Ajwain and Black Cumin leaf oils showed better anti-Candida activity against Candida albicans, resulting in an irreversible damage to the cells. The anti-Candida activity of these essential oils could be attributable to the membrane inhibition mechanism. The activity of the oils is reported to be microbicidal (Candida-cidal). PMID:25657164

  10. Susceptibility characterisation of Candida spp. to four essential oils

    Directory of Open Access Journals (Sweden)

    C C Rath

    2015-01-01

    Full Text Available In the present investigation, anti-Candida activity of four essential oils i.e. Black cumin (Nigella sativa, Curry leaf (Murraya koienigii, Ajwain (Trachiyspirum ammi, and Betel leaf (Piper betel were screened against four human pathogenic species of Candida viz. Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. The minimum inhibitory concentration (MIC values of the oils ranged between 15.62 and 250 μl/ml while studied through tube dilution method. The oils retained their anti-Candida activities even after heat treatment (at 45ΊC, 60ΊC, 100ΊC for 1 hour and also on autoclaving. Both Ajwain and Black Cumin leaf oils showed better anti-Candida activity against Candida albicans, resulting in an irreversible damage to the cells. The anti-Candida activity of these essential oils could be attributable to the membrane inhibition mechanism. The activity of the oils is reported to be microbicidal (Candida-cidal.

  11. The Candida Genome Database (CGD), a community resource for Candida albicans gene and protein information

    OpenAIRE

    Arnaud, Martha B.; Costanzo, Maria C.; Skrzypek, Marek S.; Binkley, Gail; Lane, Christopher; Miyasato, Stuart R.; SHERLOCK, Gavin

    2004-01-01

    The Candida Genome Database (CGD) is a new database that contains genomic information about the opportunistic fungal pathogen Candida albicans. CGD is a public resource for the research community that is interested in the molecular biology of this fungus. CGD curators are in the process of combing the scientific literature to collect all C.albicans gene names and aliases; to assign gene ontology terms that describe the molecular function, biological process, and subcellular localization of ea...

  12. Silver colloidal nanoparticles : antifungal effect against Candida albicans and Candida glabrata adhered cells and biofilms

    OpenAIRE

    Monteiro, D. R.; Gorup, L. F.; Silva, Sónia Carina; Negri, M.; E. R. Camargo; Oliveira, Rosário; Barbosa, D. B.; Henriques, Mariana

    2011-01-01

    The aim of this study was to evaluate the effect of silver nanoparticles (SN) against Candida albicans and Candida glabrata adhered cells and biofilms. SN (average diameter 5 nm) were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. Minimal inhibitory concentration (MIC) tests were performed for C. albicans (n = 2) and C. glabrata (n = 2) grown in suspension following the Clinical Laboratory Standards Institute microbroth dilution method. SN were applie...

  13. Hydrophobic interaction in Candida albicans and Candida tropicalis adherence to various denture base resin materials.

    OpenAIRE

    Minagi, S; Miyake, Y; Inagaki, K; Tsuru, H; Suginaka, H

    1985-01-01

    The effects of hydrophobicities of substrate surfaces on microbial adherence were examined by using Candida albicans and Candida tropicalis and 21 denture base resin materials. With increasing surface free energy of resin plates, increasing adherence of C. albicans and decreasing adherence of C. tropicalis were observed. The surface free energy of C. albicans is higher than that of all resin material surfaces, and C. tropicalis has surface free energy lower than that of all materials used. In...

  14. Production of anti-Candida antibodies in mice with gut colonization of Candida albicans.

    OpenAIRE

    Yasuo Ono; Osamu Koshio; Nobuo Suegara; Tatsuo Ikeda; Kayoko Wada; Masayasu Mitsuya; Hiroko Ishibashi; Shigeru Abe; Shigeru Tansho; Hideyo Yamaguchi

    2004-01-01

    BACKGROUND: Production of antibodies that are specific for allergens is an important pathological process in inflammatory allergic diseases. These contain the antibodies against antigens of Candida albicans, one of the normal microbial flora in an intestinal tract. We studied the effects of the prednisolone administration on the production of anti-Candida antibodies in the gastrointestinally C. albicans-colonized mice. METHODS AND MATERIALS: BALB/c mice, treated with antibacterial antibiotics...

  15. Candida albicans versus Candida dubliniensis: Why Is C. albicans More Pathogenic?

    OpenAIRE

    Moran, Gary P; Coleman, David C.; Sullivan, Derek J.

    2011-01-01

    Candida albicans and Candida dubliniensis are highly related pathogenic yeast species. However, C. albicans is far more prevalent in human infection and has been shown to be more pathogenic in a wide range of infection models. Comparison of the genomes of the two species has revealed that they are very similar although there are some significant differences, largely due to the expansion of virulence-related gene families (e.g., ALS and SAP) in C. albicans, and increased levels of pseudogenisa...

  16. Perbedaan Efek Ekstrak Jintan Hitam terhadap Candida albicans Denture Stomatitis dan Candida albicans (ATCC® 10231™)

    OpenAIRE

    Carey, Steffi

    2015-01-01

    Jintan hitam mempunyai efek fungistatis dan fungisidal. Hal ini disebabkan adanya senyawa berupa timokuinon, timol, dan karvakrol. Penelitian ini bertujuan untuk mengetahui berapa konsentrasi Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) dari ekstrak jintan hitam terhadap Candida albicans denture stomatitis dan Candida albicans (ATCC® 10231™), serta untuk mengetahui apakah terdapat perbedaan efek ekstrak jintan hitam terhadap kedua jenis fungi tersebut. Jenis penelitian eksperiment...

  17. Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis

    OpenAIRE

    Hazen, Kevin C.; Wu, Jean G.; Masuoka, James

    2001-01-01

    Although Candida dubliniensis is a close genetic relative of Candida albicans, it colonizes and infects fewer sites. Nearly all instances of candidiasis caused by C. dubliniensis are restricted to the oral cavity. As cell surface hydrophobicity (CSH) influences virulence of C. albicans, CSH properties of C. dubliniensis were investigated and compared to C. albicans. Growth temperature is one factor which affects the CSH status of stationary-phase C. albicans. However, C. dubliniensis, similar...

  18. Influence of growth conditions on cell surface hydrophobicity of Candida albicans and Candida glabrata.

    OpenAIRE

    Hazen, K C; Plotkin, B. J.; Klimas, D M

    1986-01-01

    The effect of cultural conditions on cell surface hydrophobicity of Candida albicans and Candida glabrata was tested. C. albicans cells grown at room temperature were more hydrophobic than cells grown at 37 degrees C. No consistent pattern was observed with C. glabrata. Relative hydrophobicity was found to vary with the growth phase and growth medium for both species. The implications for pathogenesis studies are discussed.

  19. Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans

    OpenAIRE

    Jackson, Andrew P; Gamble, John A.; Yeomans, Tim; Moran, Gary P.; Saunders, David; Harris, David; Aslett, Martin; Barrell, Jamie F.; Butler, Geraldine; Citiulo, Francesco; Coleman, David C.; de Groot, Piet W. J.; Goodwin, Tim J.; Quail, Michael A.; McQuillan, Jacqueline

    2009-01-01

    Candida dubliniensis is the closest known relative of Candida albicans, the most pathogenic yeast species in humans. However, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, C. dubliniensis is a significantly less virulent and less versatile pathogen. Therefore, to identify C. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the C. dubliniensis genome and compared it with the ...

  20. Structure-based specificity mapping of secreted aspartic proteases of Candida parapsilosis, Candida albicans, and Candida tropicalis using peptidomimetic inhibitors and homology modeling

    Czech Academy of Sciences Publication Activity Database

    Majer, F.; Pavlíčková, Libuše; Majer, P.; Hradilek, Martin; Dolejší, Elena; Hrušková-Heidingsfeldová, Olga; Pichová, Iva

    2006-01-01

    Roč. 387, č. 9 (2006), s. 1247-1254. ISSN 1431-6730 R&D Projects: GA ČR(CZ) GA303/04/0432; GA MŠk(CZ) LC531 Institutional research plan: CEZ:AV0Z40550506 Keywords : Candida albicans * Candida parapsilosis * Candida inhibitors * secreted aspartic protease Subject RIV: CE - Biochemistry Impact factor: 2.752, year: 2006

  1. Candida Esophagitis in an Immunocompetent Pregnant Woman

    Directory of Open Access Journals (Sweden)

    Jeffrey S. Greenspoon

    1993-01-01

    Full Text Available Background: Nausea and vomiting are common during the first half of pregnancy and usually require only supportive measures. When symptoms are progressive and weight loss occurs, treatable causes should be sought by means of upper gastrointestinal endoscopy. We report a case of an immunocompetent gravida with invasive Candida albicans esophagitis.

  2. Intracellular aspartic protease of Candida albicans

    Czech Academy of Sciences Publication Activity Database

    Bauerová, Václava; Pichová, Iva; Hrušková-Heidingsfeldová, Olga

    Mátraháza : -, 2007. s. 43. [Alexander Von Humboldt Workshop on Structure Based Approaches Towards Disease Control. 22.05.2007-27.05.2007, Mátraháza] Institutional research plan: CEZ:AV0Z40550506 Keywords : Candida parapsilosis * intracellular * aspartic protease Subject RIV: CE - Biochemistry

  3. Intracellular aspartic protease ACP of Candida albicans

    Czech Academy of Sciences Publication Activity Database

    Bauerová, Václava; Dolejší, Elena; Hrušková-Heidingsfeldová, Olga; Pichová, Iva

    Patras : University of Patras, 2007. s. 306. [General Meeting of the International Proteolysis Society /5./. 20.10.2007-24.10.2007, Patras] R&D Projects: GA ČR GA203/05/0038; GA MŠk(CZ) LC531 Institutional research plan: CEZ:AV0Z40550506 Keywords : Candida albicans * ACP Subject RIV: CE - Biochemistry

  4. 21 CFR 173.160 - Candida guilliermondii.

    Science.gov (United States)

    2010-04-01

    ... citric acid produced conforms to the specifications of the “Food Chemicals Codex,” 3d Ed. (1981), under... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Candida guilliermondii. 173.160 Section 173.160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED)...

  5. Imunidade conferida por vacinas anti-meningocócicas

    Directory of Open Access Journals (Sweden)

    Milagres Lucimar Gonçalves

    1993-01-01

    Full Text Available Em razão da recente epidemia de doença meningocócica causada por N. meningitidis B na Grande São Paulo, Brasil, foi feita revisão das epidemias dessa doença ocorridas no Brasil desde o início do século e uma análise das vacinas atuais contra N. meningitidis A, C, Y e W135. Também são discutidos os mais recentes avanços no desenvolvimento e aplicação de vacina contra M meningitidis B, um desafio constante para os maiores centros de pesquisa de todo o mundo.

  6. Mastite por leveduras em bovinos leiteiros do Sul do Estado de Minas Gerais, Brasil Mastitis caused by yeasts in dairy herds in the South of the Minas Gerais State, Brazil

    Directory of Open Access Journals (Sweden)

    Geraldo Márcio da Costa

    2008-10-01

    Full Text Available O objetivo deste trabalho foi estudar a freqüência de infecções intramamárias ocasionadas por leveduras a partir de amostras de leite (n=1710 coletadas em 40 rebanhos leiteiros do Estado de Minas Gerais. Cinqüenta e seis estirpes de leveduras do gênero Candida e uma linhagem de Trichosporon loubieri foram isoladas. Candida albicans foi a espécie dominante (28,1% das cepas, seguida por Candida parapsilosis (19,3%, Candida catenulata (14,0%, Candida glabrata (14,0% e Candida tropicalis (8,8%. Infecções mistas foram detectadas em 29,8% das vacas levedura-positivas. Amostras positivas para leveduras foram predominantemente obtidas (84% de vacas com mastite subclínica. A baixa taxa de isolamento de leveduras sugere que estes microrganismos não são relevantes para mastite bovina na região estudada.The objective of this study was to report the frequency of intramammary infections by yeasts, in Minas Gerais State, from milk samples (n = 1710 collected in 40 dairy herds. Fifty six yeast strains of the genus Candida and one strain of Trichosporon loubieri were isolated. Candida albicans was the dominant species (28.1% of the strains, followed by Candida parapsilosis (19.3%, Candida catenulata (14.0%, Candida glabrata (14.0%, Candida tropicalis (8.8%. Mixed infections were detected in 29.8% of yeast-positive cows. The yeast infection was more frequent (84% in cows with subclinical mastitis. The low rate of isolation of yeasts suggests that these microorganisms are not relevant to bovine mastitis in the studied region.

  7. Incidência da varíola, causada por Asperisporium caricae, em folhas de mamoeiros submetidos ao manejo orgânico, em diferentes ambientes de cultivo.

    OpenAIRE

    Luiz Aurélio Peres Martelleto; Raul de Lucena Duarte Ribeiro; Margarida Goréte Ferreira do Carmo; Mariluci Sudo-Martelleto; Antonio de Goes

    2009-01-01

    Mudas de mamoeiro da cultivar Baixinho de Santa Amália foram transplantadas para covas de 40x60x40 cm, em áreas de três estruturas contíguas: (a) estufa sombreada (cobertura de plástico), (b) estufa sombreada + sombrite (cobertura adicional de sombrite com 30% de sombreamento sobre o plástico) e (c) telado (cobertura exclusiva de sombrite 30%). Ao lado de tais estruturas foi implantada uma área de cultivo de mamoeiro em ambiente natural. Os tratos culturais aplicados foram os condizentes às n...

  8. Boro, nitrogenio concentraçao de inóculo de pH na expressao da doença causada por Plasmodiophora brassicae

    OpenAIRE

    Schuta, Lucimeris Ruaro

    2013-01-01

    Resumo: A hérnia das cruciferas (Plasmodiophora brassicae Woron.) é a mais importante doença que incide sobre as variedades de Brassica oleracea L. representadas pelo repolho, couve-flor, brócolis, mostarda, couve-chinesa e rúcula. A calagem para elevar o pH dos solos é a medida recomendada mais aceita pelos produtores, contudo, nem sempre esta medida, é suficiente para impedir ou reduzir o ataque do patógeno nos solos da região metropolitana de Curitiba. O que sugere, que outros fatores poss...

  9. REAÇÃO DE CULTIVARES DO FEIJOEIRO COMUM ÀS PODRIDÕES RADICULARES CAUSADAS POR Rhizoctonia solani E Fusarium solani f. sp. phaseoli

    Directory of Open Access Journals (Sweden)

    Braycia Afonso de Miranda

    2007-12-01

    Full Text Available REACTION OF COMMON BEAN CULTIVARS TOROOT ROT CAUSED BY Rhizoctonia solaniAND Fusarium solani f. sp. phaseoliRhizoctonia solani Kuhn is a necrotrophic fungus andsoil inhabitant that attacks great number of vegetable species. Inbean plants, R. solani can induce different symptoms includingtoppling, root and colon plant rottenness. Fusarium solani (MartSacc. f. sp. phaseoli (Burcholder occurs in practically all beanproducing areas in Brazil and can cause root rots and death tobean plants. This study evaluated the reaction of commercialcommon bean cultivars growing in R. solani or F. solani f. sp.phaseoli infested soil, under green house conditions. Theexperimental design was entirely randomized, with 24 treatmentsand four replications. A Oxisol was infested with trituratedsorghum grains that were previously colonized by R. solani (1.0g/1.4 L of soil or F. solani f. sp phaseoli (8.0 g/1.4 L of soil. Thedisease evaluations were accomplished 21 days after planting.All plants were carefully removed, their root systems werewashed in running water and the disease severity was evaluatedaccording to Abawi & Pastor-Corrales (1990 scale for diseaseseverity. The cultivar behavior ranged according to the inoculatedpathogen, all cultivars were susceptible with different degrees ofsusceptibility. Among evaluated common bean cultivars Pérolawas less susceptible to R. solani, and Radiante was lesssusceptible to F. solani f. sp. phaseoli.

  10. Podridão de frutos de melancia causada por Sclerotium rolfsii no estado de Alagoas Watermelon fruit rot caused by Sclerotium rolfsii in the State of Alagoas

    Directory of Open Access Journals (Sweden)

    FÁTIMA M. QUEIROZ

    2002-09-01

    Full Text Available A new fruit rot disease caused by Sclerotium rolfsii on watermelon (Citrullus lanatus was observed in the State of Alagoas. The pathogenicity of the fungus was confirmed by artificial inoculations.This is the first report of S. rolfsii causing fruit rot in watermelon in Northeast of Brazil.

  11. Podridão de frutos de melancia causada por Sclerotium rolfsii no estado de Alagoas Watermelon fruit rot caused by Sclerotium rolfsii in the State of Alagoas

    OpenAIRE

    Fátima M. Queiroz; MUNIZ M. F. S.; MARIA MENEZES; PERICLES G. BARROS

    2002-01-01

    A new fruit rot disease caused by Sclerotium rolfsii on watermelon (Citrullus lanatus) was observed in the State of Alagoas. The pathogenicity of the fungus was confirmed by artificial inoculations.This is the first report of S. rolfsii causing fruit rot in watermelon in Northeast of Brazil.

  12. Cerebelite aguda causada por vírus Epstein-Barr: relato de caso Acute cerebellitis caused by Epstein-Barr virus: case report

    Directory of Open Access Journals (Sweden)

    Hélio A.G. Teive

    2001-09-01

    Full Text Available A cerebelite aguda pode ocorrer em associação a infecção pelo vírus da varicela-zoster, enterovirus, caxumba, micoplasma e outros agentes infecciosos. A cerebelite aguda é uma complicação rara da infecção pelo vírus Epstein-Barr (EBV. Relatamos o caso de uma mulher de 21 anos com história de 12 dias de evolução com náuseas, vômitos, ataxia de marcha e membros, tremor cefálico e de membros, opsoclono, mioclonias e rash cutâneo. Sorologia para EBV foi positiva. A infecção pelo EBV, com complicações neurológicas, pode não se apresentar com os sinais e sintomas clássicos da mononucleose infeciosa.Acute cerebellitis can occur in association with varicella-zoster virus, enterovirus, mumps, mycoplasma, and other infective organisms. Acute cerebellitis is a rare complication of Epstein-Barr virus (EBV infection. We report the case of a 21-year-old woman with a 12-day history of nausea and vomiting, gait and limbs ataxia, myoclonus, tremor of head and all four limbs, opsoclonus and cutaneous rash. Anti-EBV IgG and IgM antibodies against antiviral capsid were positive and anti-EBV against virus-associated nuclear antigen was also positive. EBV infection in association with neurological findings can occur without the classic signs and symptoms of infectious mononucleosis.

  13. Subcutaneous phaeohyphomycosis caused by Bipolaris hawaiiensis: a case report Feohifomicose subcutânea causada por Bipolaris hawaiiensis: registro de um caso

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    Alcyr Ribeiro Costa

    1991-02-01

    Full Text Available A case of phaeohyphomycosis caused by Bipolaris hawaiiensis is reported. The patient, an immunocompetent host, presented a verrucous lesion on the first finger of the left foot. Dematiaceous septate hyphae and yeast-like elements were seen in direct and histological examination. The isolated strain was identified on the basis of micro and macromorphological aspects. Treated with electrocoagulation, the lesions healed and presented no relapse after two years follow-up.Os autores descrevem um caso de feohifomicose com lesão verrucosa no hálux esquerdo. O paciente não apresentava sinais clínicos de deficiência imunológica. Os exames direto e histopatológico mostraram células leveduriformes e poucas hifas septadas, demacióides, essenciais ao diagnóstico desta micose. O cultivo em lâmina permitiu identificar o hifomiceto como Bipolaris hawaiiensis. A eletrocoagulação foi usada como tratamento, com cura do processo, não havendo recidiva após dois anos.

  14. Primeiro relato de mancha foliar em Cedrela odorata causada por Pseudobeltrania cedrelae First report of leaf spot on Cedrela odorata caused by Pseudobeltrania cedrelae

    Directory of Open Access Journals (Sweden)

    Rogério E. Hanada

    2005-06-01

    Full Text Available Manchas foliares e desfolha foram constatadas em árvores de cedro (Cedrela odorata estabelecidas em uma área experimental da Embrapa Amazônia Ocidental, Manaus-AM. Inicialmente nas folhas novas surgem halos cloróticos rodeando pequenas lesões. Posteriormente surgem lesões necróticas, circulares a angulares, A disease causing leaf spot and defoliation on Cedrela odorata trees planted in an Embrapa experimental area near Manaus, Brazil, is first reported here. Lesions start as yellow halos surrounding small light-brown points. Later, necrotic brown lesions are observed exhibiting many black small structures in the central portion, corresponding to fructifications of Pseudobeltrania cedrelae and Colletotrichum gloeosporioides. When P. cedrelae was inoculated the leaf disease was reproduced.

  15. Aportaciones a la epidemiologia y a las manifestaciones clínicas de las sobredosis causadas por la cocaina y las nuevas drogas de diseño

    OpenAIRE

    Galicia Paredes, Miguel Ángel

    2010-01-01

    [spa] De los datos disponibles en la actualidad, se puede concluir que el consumo de sustancias de abuso genera un número importante de problemas de salud, de presentación aguda o crónica. Las consecuencias del consumo prolongado de diferentes sustancias, y su repercusión sobre la salud del consumidor o de su entorno social son bien conocidas (enfermedades en ADVP, marginalidad de zonas de comercio de sustancias, políticas dirigidas a disminuir el tráfico de sustancias). Sin embargo no hay ta...

  16. Gomose da acácia-negra causada por Ceratocystis fimbriata Ell. & Halst. Gummosis of Acacia decurrens Willd. Caused by Ceratocystis fimbriata Ell. & Halst.

    OpenAIRE

    Ivan José Antunes Ribeiro; Margarida Fumikoito; Osvaldo Paradela Filho; Jairo Lopes de Castro

    1988-01-01

    É relatada a ocorrência, pela primeira vez no Brasil, de Ceratocystis fimbriata Ell. & Halst. sobre acácia-negra. Testes de inoculação cruzada foram efetuados em casa de vegetação com o isolado obtido de acácia-negra (Acacia decurrens Willd.) e outro de mangueira (Mangifera indica L.). Observou-se que ambos os isolados foram patogênicos à acácia-negra e à mangueira.Several plants of Acacia decurrens showed wilting and subsequent branche drying. The branches showed wood splitting and gum exuda...

  17. El régimen internacional de la bioinvasión marina causada por agua de lastre: especial referencia a la República de Colombia

    OpenAIRE

    Ramírez Cabrales, Fabián

    2015-01-01

    El agua de lastre de los buques es considerada como el más importante vector para el desplazamiento transoceánico de especies marinas propias de aguas superficiales a nuevos ambientes marinos. Se ha estimado que anualmente se transfieren de 3 a 5 billones de toneladas de agua de lastre a nivel global. Ello plantea un serio riesgo ambiental internacional. Las especies invasivas están extendiendo sus ámbitos naturales a nivel global y cada nueve semanas se notifica una nueva invasión biológica ...

  18. Bases moleculares para la prevención de una enfermedad causada por Bourdella pertussis, un patógeno re emergente

    OpenAIRE

    Bottero, Daniela

    2010-01-01

    En Argentina la vacunación contra la tos convulsa comienza en los años setenta con la incorporación masiva de la vacuna celular triple bacteriana (DTP – Difteria, Tétanos y Pertussis) en el Calendario Nacional de Vacunación con tres dosis a los 2, 4 y 6 meses y un refuerzo a los 18 meses. La situación epidemiológica de la enfermedad motivó en los años ochenta la incorporación de un refuerzo al ingreso escolar y en el año 2009 un refuerzo en la población adolescente. Se espera que estas estrat...

  19. REAÇÃO DE CULTIVARES DO FEIJOEIRO COMUM ÀS PODRIDÕES RADICULARES CAUSADAS POR Rhizoctonia solani E Fusarium solani f. sp. phaseoli

    OpenAIRE

    Braycia Afonso de Miranda; Murillo Lobo Júnior; Marcos Gomes Cunha

    2007-01-01

    REACTION OF COMMON BEAN CULTIVARS TOROOT ROT CAUSED BY Rhizoctonia solaniAND Fusarium solani f. sp. phaseoliRhizoctonia solani Kuhn is a necrotrophic fungus andsoil inhabitant that attacks great number of vegetable species. Inbean plants, R. solani can induce different symptoms includingtoppling, root and colon plant rottenness. Fusarium solani (Mart)Sacc. f. sp. phaseoli (Burcholder) occurs in practically all beanproducing areas in Brazil and can cause root rots and death tobean plants. This...

  20. Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

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    Helio Langoni

    2015-06-01

    Full Text Available Resumo: A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado de São Paulo. Foram examinadas 1148 vacas em lactação, totalizando 4584 glândulas mamárias. Foram considerados os casos, em que houve isolamento de estafilococos coagulase positiva (SCP e estafilococos coagulase negativa (SCN. Os resultados revelaram microbiota com vários patógenos e diferentes espécies de SCN (128 casos e SCP (45, Staphylococcus aureus(90, Streptococcus agalactiae(70, Streptococcus dysgalactiae (69, Streptococcus uberis(29, Corynebacteriumspp. (230, Klebsiella pneumoniae(28, Klebsiella oxytoca(2, Escherichia coli(15, Enterobactersp. (3. Os resultados de contagem de células somáticas (CCS relacionados aos SCP e SCN não mostraram diferenças entre as propriedades avaliadas, entretanto com diferenças significantes ao se avaliar a CCS entre os dois grupos de estafilococos, como pode ser evidenciado ao comparar SCN Discreto e SCP exuberante (P<0,01, SCP Discreto e SCP exuberante (P<0,001 e SCN moderado e SCP exuberante (P<0,01. A avaliação da CCS relacionada à intensidade da infecção, considerando-se como crescimento discreto o isolamento de até nove colônias, moderado de dez a 29 colônias e exuberante, com 30 ou mais colônias, revelou para ambos os grupos de estafilococos que quanto maior o número de unidades formadoras de colônias (UFC, a CCS é mais elevada, sendo sempre maior nos casos de SCP. Conclui-se que quando há maior número de UFC, há concomitantemente maior CCS/mL de leite, no caso dos SCP e SCN, o que mostra relação direta da intensidade do processo infeccioso com a resposta da celularidade do leite, bem como pela relevância desses na etiologia das mastites e dos aspectos negativos tanto para a produção, quanto na qualidade do leite produzido nas propriedades.

  1. In vitro activity of Caspofungin combined with Fluconazole on mixed Candida albicans and Candida glabrata biofilm.

    Science.gov (United States)

    Pesee, Siripen; Angkananuwat, Chayanit; Tancharoensukjit, Sudarat; Muanmai, Somporn; Sirivan, Pattaraporn; Bubphawas, Manita; Tanarerkchai, Nissara

    2016-05-01

    The objective of this study was to evaluate the antifungal effect of caspofungin (CAS) combined with fluconazole (FLU) on the biofilm biomass and cultivable viability and microstructure ofCandida albicansandCandida glabratamixed biofilmin vitro.Biofilms were formed in a 96-well microtiter plate for crystal violet assay and colony forming unit (CFU) method and grown on plastic coverslip disks for scanning electron microscopy. MIC50of CAS and FLU against singleCandida spp.and mixedCandida spp.biofilms were evaluated using crystal violet assay. Additional,C. albicansandC. glabratamixed biofilms were incubated with subinhibitory CAS concentration plus FLU and their percentages ofCandidabiofilm reduction were calculated. We found that percentages of biofilm reduction were significantly decreased when CAS at 0.25MIC and FLU (0.25 or 0.5MIC) were combined (P< .05) but not different when CAS at 0.5 MIC combined with FLU at 0.25 or 0.5MIC, compared to CAS treatment alone. Structural analyses revealed that CAS/FLU combination-treated biofilms showed less hyphae and blastospores with some aberrant cells compared to control group. Although it was evident that a greater CFU ofCandida glabratawere demonstrated in every group, the total viable cells derived from CAS/FLU combination-treated biofilms at any ratio were not significantly different from positive control. Overall, CAS/FLU combinations appeared to affect the quantity and cell architecture, but number of viable cell, ofCandida albicansandCandida glabratamixed biofilm. This antifungal effect was CAS concentration dependent. PMID:26768371

  2. Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle Isolamento de Candida spp da microbiota vaginal de fêmeas caninas hígidas durante o ciclo estral

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    Marlete Brum Cleff

    2005-06-01

    Full Text Available Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and other mucosa in mammalians. Candida spp has been frequently isolated from domestic and sylvan animals. Yeast isolation from vaginal mucus is frequent in women, especially of Candida genus. In canine females, however, studies about vaginal fungal microbiota are rare and the connection with estrous cycle is unknown. The purpose of this study was to isolate yeast 5 of Candida genus from healthy canine females and identify the isolated species, establishing their connection with estrous cycle phases. Two hundred twenty-four samples taken from vaginal mucus of 14 canine females were analyzed in this study. Candida spp was observed in 83 (37% from these samples; nine samples were taken in proestrus, 14 in oestrus, 31 in dioestrus, 24 in anoestrus and five samples were obtained during pregnancy. Candida parapsilosis the most frequently isolated specie (21.7%, followed by C. guillermondii (8.4%, C. kefir (6% and C. albicans (4.8%. We concluded that Candida spp is a commion component of healthy canine females' vaginal microbiota, and isolation varies according to the estrous cycle phase.As leveduras são organismos comensais da pele, trato genital e gastrointestinal, e de outras mucosas de mamíferos. O gênero Candida vem sendo isolado freqüentemente de animais domésticos e silvestres. O isolamento de Candida spp da mucosa vaginal de mulheres é freqüente, porém na espécie canina são escassos os estudos referentes à microbiota fúngica vaginal, especialmente do gênero candida, não se tendo conhecimento de sua relação com o ciclo reprodutivo. O presente trabalho teve como objetivo isolar leveduras do gênero Candida em fêmeas caninas hígidas e identificar as espécies isoladas, relacionando-as com as diferentes fases do ciclo estral. Foram analisadas 224 amostras obtidas da mucosa vaginal de 14 fêmeas caninas. Candida spp foi observada em 83 (37

  3. Candida heliconiae sp. nov., Candida picinguabensis sp. nov. and Candida saopaulonensis sp. nov., three ascomycetous yeasts from Heliconia velloziana (Heliconiaceae).

    Science.gov (United States)

    Ruivo, Carla C C; Lachance, Marc-André; Rosa, Carlos A; Bacci, Maurício; Pagnocca, Fernando C

    2006-05-01

    Strains belonging to three novel yeast species, Candida heliconiae (four isolates), Candida picinguabensis (three isolates) and Candida saopaulonensis (two isolates), were recovered in the year 2000 from water of flower bracts of Heliconia velloziana L. Emigd. (Heliconiaceae) found in a forest ecosystem site in an Atlantic rainforest of south-eastern Brazil. C. picinguabensis and C. saopaulonensis were nearly identical in morphology and physiology, but sequence divergence in the D1/D2 domain of the large-subunit rDNA indicated that they should be regarded as different species. They belong to the Metschnikowiaceae clade. C. heliconiae had affinities to Pichia mexicana and related species, but was genetically isolated from all currently accepted species in that group. The type strains are C. heliconiae UNESP 00-91C1T (=CBS 10000T=NRRL Y-27813T), C. picinguabensis UNESP 00-89T (=CBS 9999T=NRRL Y-27814T) and C. saopaulonensis UNESP 00-99T (=CBS 10001T=NRRL Y-27815T). PMID:16627669

  4. Identification and characterization of nine atypical Candida dubliniensis clinical isolates.

    Science.gov (United States)

    Albaina, Olatz; Sahand, Ismail H; Brusca, María I; Sullivan, Derek J; Fernández de Larrinoa, Iñigo; Moragues, María D

    2015-02-01

    Candida dubliniensis is a pathogenic yeast of the genus Candida closely related to Candida albicans. The phenotypic similarity of these two species often leads to misidentification of C. dubliniensis isolates in clinical samples. DNA-based methods continue to be the most effective means of discriminating accurately between the two species. Here, we report on the identification of nine unusual Candida isolates that showed ambiguous identification patterns on the basis of their phenotypic and immunological traits. The isolates were categorized into two groups. Group I isolates were unable to produce germ tubes and chlamydospores, and to agglutinate commercial latex particles coated with a mAb highly specific for C. dubliniensis. Group II isolates grew as pink and white colonies on CHROMagar Candida and ChromID Candida, respectively. Carbohydrate assimilation profiles obtained with API/ID32C together with PCR amplification with specific primers and DNA sequencing allowed reliable identification of the nine unusual clinical isolates as C. dubliniensis. PMID:25480879

  5. Screening of Tanzanian medicinal plants for anti-Candida activity

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    Joseph Cosam C

    2006-03-01

    Full Text Available Abstract Background Candida albicans has become resistant to the already limited, toxic and expensive anti-Candida agents available in the market. These factors necessitate the search for new anti-fungal agents. Methods Sixty-three plant extracts, from 56 Tanzanian plant species obtained through the literature and interviews with traditional healers, were evaluated for anti-Candida activity. Aqueous methanolic extracts were screened for anti-Candida activity by bioautography agar overlay method, using a standard strain of Candida albicans (ATCC 90028. Results Twenty- seven (48% out of the 56 plants were found to be active. Extracts of the root barks of Albizia anthelmintica and Balanites aegyptiaca, and roots of Plectranthus barbatus showed strong activity. Conclusion The extracts that showed strong anti-Candida activity are worth of further investigation in order to isolate and identify the active compounds.

  6. Infecção por Treponema pallidum: análise serológica e pesquisa de DNA

    OpenAIRE

    Ferreira, Lino José Manuel

    2013-01-01

    RESUMO: A sífilis é uma infecção causada por T. pallidum que pode ser transmitida por via vertical, por contacto sexual ou por sangue, e cujo diagnóstico se baseia na associação entre manifestações clinicas e testes serológicos. Neste estudo utilizaram-se os testes serológicos RPR, TPHA, FTA-Abs, um teste rápido não comercializado (Signal-Spirolipin) que pesquisa anticorpos treponémicos (CDC-T) e não treponémicos (CDC-2) em simultâneo no mesmo dispositivo e uma técnica de PC...

  7. Candida Arthritis: Analysis of 112 Pediatric and Adult Cases

    OpenAIRE

    Gamaletsou, Maria N; Rammaert, Blandine; Bueno, Marimelle A.; Sipsas, Nikolaos V.; Moriyama, Brad; Kontoyiannis, Dimitrios P.; Roilides, Emmanuel; Zeller, Valerie; Taj-Aldeen, Saad J; Miller, Andy O.; Petraitiene, Ruta; Lortholary, Olivier; Thomas J Walsh

    2015-01-01

    Background.  Candida arthritis is a debilitating form of deeply invasive candidiasis. However, its epidemiology, clinical manifestations, management, and outcome are not well understood. Methods.  Cases of Candida arthritis were reviewed from 1967 through 2014. Variables included Candida spp in joint and/or adjacent bone, underlying conditions, clinical manifestations, inflammatory biomarkers, diagnostic imaging, management, and outcome. Results.  Among 112 evaluable cases, 62% were males and...

  8. Crystal violet staining to quantity Candida adhesion to epithelial cells

    OpenAIRE

    Negri, M.; Gonçalves, Vera M.; Silva, Sónia Carina; Henriques, Mariana; Azeredo, Joana; Oliveira, Rosário

    2010-01-01

    In vitro studies of adhesion capability are essential to characterise the virulence of Candida species. However, the assessment of adhesion by traditional methods is timeconsuming. The aim of the present study is the development of a simple methodology using crystal violet staining to quantify in vitro adhesion of different Candida species to epithelial cells. The experiments are performed using Candida albicans (ATCC 90028), C. glabrata (ATCC 2001), C. parapsilosis (ATCC 22019) and C. tropic...

  9. Identification of Candida spp. by phenotypic tests and PCR

    OpenAIRE

    Sandra Aparecida Marinho; Alice Becker Teixeira; Otávio Silveira Santos; Ricardo Flores Cazanova; Carlos Alexandre Sanchez Ferreira; Karen Cherubini; Sílvia Dias de Oliveira

    2010-01-01

    The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production,...

  10. Candida bracarensis Bloodstream Infection in an Immunocompromised Patient ▿

    OpenAIRE

    Warren, Thomas A.; McTaggart, Lisa; Richardson, Susan E.; Zhang, Sean X.

    2010-01-01

    Candida bracarensis is a recently described Candida species which is phenotypically similar to Candida glabrata. A case of C. bracarensis bloodstream infection in a bone marrow transplant patient is described and confirms this organism as an opportunistic human pathogen. The organism can be distinguished from C. glabrata by its white color on CHROMagar and by DNA sequence analysis using D1/D2 and internal transcribed spacer (ITS) primers.

  11. Activity of phenolic compounds from plant origin against Candida species

    OpenAIRE

    Martins, Natália; Barros, Lillian; Henriques, Mariana; Silva, Sónia; Ferreira, Isabel C. F. R.

    2015-01-01

    Candida albicans and other Candida species have been highly associated with several opportunistic fungal infections. Their ability to develop host infections is incited by different determinants, being virulence factors the most highlighted. Molecular targets of the antifungal drugs are crucial components for determination of yeast survival. Ergosterol, nucleic acids and glucan are the most studied molecular targets to destroy Candida species, being considered the basis of the development of ...

  12. Roles of Candida albicans Sfl1 in Hyphal Development▿

    OpenAIRE

    Li, Yandong; Su, Chang; Mao, Xuming; Cao, Fang; Chen, Jiangye

    2007-01-01

    The ability to switch between different morphological forms is an important feature of Candida albicans and is relevant to its pathogenesis. Many conserved positive and negative transcription factors are involved in morphogenetic regulation of the two dimorphic fungi Candida albicans and Saccharomyces cerevisiae. In S. cerevisiae, the transcriptional repressor Sfl1 and the activator Flo8 function antagonistically in invasive and filamentous growth. We have previously reported that Candida alb...

  13. Oral candidiasis-adhesion of non-albicans Candida species

    OpenAIRE

    Bokor-Bratić Marija B.

    2008-01-01

    Oral candidiasis is an opportunistic infection caused primarily by Candida albicans. However, in recent years, species of non-albicans Candida have been implicated more frequently in mucosal infection. Candida species usually reside as commensal organisms and are part of normal oral microflora. Determining exactly how transformation from commensal to pathogen takes place and how it can be prevented is continuous challenge for clinical doctors. Candidal adherence to mucosal surfaces is conside...

  14. Rat Indwelling Urinary Catheter Model of Candida albicans Biofilm Infection

    OpenAIRE

    Nett, Jeniel E.; Brooks, Erin G.; Cabezas-Olcoz, Jonathan; Sanchez, Hiram; Zarnowski, Robert; Marchillo, Karen; Andes, David R.

    2014-01-01

    Indwelling urinary catheters are commonly used in the management of hospitalized patients. Candida can adhere to the device surface and propagate as a biofilm. These Candida biofilm communities differ from free-floating Candida, exhibiting high tolerance to antifungal therapy. The significance of catheter-associated candiduria is often unclear, and treatment may be problematic considering the biofilm drug-resistant phenotype. Here we describe a rodent model for the study of urinary catheter-a...

  15. Absence of Amphotericin B-Tolerant Persister Cells in Biofilms of Some Candida Species▿

    OpenAIRE

    Al-Dhaheri, Rawya S.; Douglas, L. Julia

    2008-01-01

    Biofilms and planktonic cells of five Candida species were surveyed for the presence of persister (drug-tolerant) cell populations after exposure to amphotericin B. None of the planktonic cultures (exponential or stationary phase) contained persister cells. However, persisters were found in biofilms of one of two strains of Candida albicans tested and in biofilms of Candida krusei and Candida parapsilosis, but not in biofilms of Candida glabrata or Candida tropicalis. These results suggest th...

  16. Investigative Methods for Studying the Adhesion and Cell Surface Hydrophobicity of Candida Species: An Overview

    OpenAIRE

    Ellepola, Arjuna N. B.; Samaranayake, Lakshman P.

    2011-01-01

    Candidal infections are common opportunistic infections in the compromized and manifest both as super? cial and systemic diseases. The super?ficial infections are by far the commonest form of the disease. Although Candida albicans is the most common Candida species isolated from humans and is responsible for the majority of super? cial yeast infections, non-albicans species such as Candida krusei, Candida glabrata, Candida parapsilosis and Candida tropicalis are regularly isolated but to a le...

  17. VULVOVAGINAL CANDIDA INFECTION PREVALENCE IN TASHKENT

    Directory of Open Access Journals (Sweden)

    Uktam Ziyadullaev

    2013-10-01

    Full Text Available Background: The information on the prevalence of vulvovaginal candidiasis does not always reflect real situation regarding this disease, since the frequency of patients’ self- treatment remains high, as evidenced by the results of the studies based on anonymous surveys. The prevalence of this disease is growing steadily both in Uzbekistan and elsewhere in the world.  Accumulated problems have provided grounds to conduct the research on the prevalence of Candida vulvovaginitis in the juvenile age population of Tashkent city. Method: The study included examining of 2107 adolescent aged girls of high schools, lyceums and colleges of Tashkent city. Results: Thus, in the studied region the prevalence of Candida vulvovaginitis in adolescent population is high, which in turn requires to take steps to further improve treatment and prevention.  

  18. Choque séptico por ehrliquiosis

    Directory of Open Access Journals (Sweden)

    Viviana Hernández-de Mezerville

    2007-04-01

    Full Text Available La ehrliquiosis humana es una infección zoonótica transmitida por "garrapatas duras" causada por Anaplasma sp y Ehrlichia sp. Puede presentarse como un cuadro pseudogripal con fiebre, malestar general, mialgias y cefalea o hasta un choque séptico con falla multiorgánica y muerte. La mitad de los pacientes requieren atención hospitalaria en algún momento y se reporta una mortalidad de hasta el 5% secundaria a enfermedades oportunistas o a hemorragias. El diagnóstico se puede sospechar visualizando las mórulas intracitoplasmáticas en las células afectadas en frotis de sangre periférica, y se confirma con serología, con cultivos o con detección molecular. El tratamiento de elección es la doxiciclina con buena respuesta clínica. Se reporta un caso probable de ehrliquiosis humana asociado a choque séptico y falla multiorgánica tratado exitosamente en el Hospital Dr. Rafael Angel Calderón Guardia.Human ehrlichiosis is a tick-borne infectious disease caused by Anaplasma sp and Ehrlichia sp. The presentation can range from a flu-like syndrome with fever, malaise, myalgia and headache to a septic shock state with multiorganic dysfunction. Half the patients require in- hospital care and the mortality rate is 5% as a result of opportunistic infections or hemorrhage. The diagnosis can be made by the visualization of intracytoplasmatic morulae in the blood smear, serology, cultives or polymerase chain reaction. The treatment of choice is doxycicline and generally carries a good response. We report a case of human ehrlichiosis associated with septic shock and multiorganic dysfunction successfully treated at the Calderon Guardia Hospital.

  19. Proinflammatory Chemokines during Candida albicans Keratitis

    OpenAIRE

    Yuan, Xiaoyong; Hua, Xia; Wilhelmus, Kirk R.

    2009-01-01

    Chemotactic cytokines mediate the recruitment of leukocytes into infected tissues. This study investigated the profile of chemokines during experimental Candida albicans keratitis and determined the effects of chemokine inhibition on leukocyte infiltration and fungal growth during murine keratomycosis. Scarified corneas of BALB/c mice were topically inoculated with C. albicans and monitored daily over one week for fungal keratitis. After a gene microarray for murine chemokines compared infect...

  20. Tetracycline Effects on Candida Albicans Virulence Factors

    OpenAIRE

    Logan McCool; Hanh Mai; Michael Essmann; Bryan Larsen

    2008-01-01

    Object. To determine if tetracycline, previously reported to increase the probability of developing symptomatic vaginal yeast infections, has a direct effect on Candida albicans growth or induction of virulent phenotypes. Method. In vitro, clinical isolates of yeast were cultivated with sublethal concentrations of tetracycline and yeast cell counts, hyphal formation, drug efflux pump activity, biofilm production, and hemolysin production were determined by previously reported methods. Resul...

  1. Pharmacotherapy of Candida Infections with Echinocandins

    OpenAIRE

    Ana Espinel-Ingroff; Emilia Canton; Estrella Martin-Mazuelos; Javier Pemán

    2009-01-01

    The classic recommended antifungal agents for the treatment of invasive Candida infections were amphotericin B, a lipid formulation of amphotericin B and fluconazole in both neutropenic or nonneutropenic patients as either primary or alternative therapies. Voriconazole has been recommended when additional coverage for filamentous fungi is needed (e.g. neutropenic patients). More recently and based on well designed comparative clinical trials, the three echinocandins, caspofungin, anidulafungi...

  2. Candida Esophagitis in an Immunocompetent Pregnant Woman

    OpenAIRE

    Greenspoon, Jeffrey S.; Seth Kivnick

    1993-01-01

    Background: Nausea and vomiting are common during the first half of pregnancy and usually require only supportive measures. When symptoms are progressive and weight loss occurs, treatable causes should be sought by means of upper gastrointestinal endoscopy. We report a case of an immunocompetent gravida with invasive Candida albicans esophagitis. Case: The immunocompetent primigravida developed progressive nausea, vomiting, epigastric pain, and a 4.1 kg weight loss during the second trimester...

  3. VULVOVAGINAL CANDIDA INFECTION PREVALENCE IN TASHKENT

    OpenAIRE

    Uktam Ziyadullaev

    2013-01-01

    Background: The information on the prevalence of vulvovaginal candidiasis does not always reflect real situation regarding this disease, since the frequency of patients’ self- treatment remains high, as evidenced by the results of the studies based on anonymous surveys. The prevalence of this disease is growing steadily both in Uzbekistan and elsewhere in the world.  Accumulated problems have provided grounds to conduct the research on the prevalence of Candida vulvovaginitis in the juvenile ...

  4. Candida parapsilosis Biofilm Identification by Raman Spectroscopy

    OpenAIRE

    Ota Samek; Katarina Mlynariková; Silvie Bernatová; Jan Ježek; Vladislav Krzyžánek; Martin Šiler; Pavel Zemánek; Filip Růžička; Veronika Holá; Martina Mahelová

    2014-01-01

    Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to...

  5. Laminin receptors on Candida albicans germ tubes.

    OpenAIRE

    Bouchara, J P; Tronchin, G; Annaix, V; Robert, R; Senet, J M

    1990-01-01

    Recent evidence for the role of laminin in cell adhesion and in the pathogenesis of several bacterial infections has led us to investigate the existence of receptors for this extracellular matrix component in Candida albicans. At first, immunofluorescence demonstrated the presence of laminin-binding sites at the surface of germ tubes. Electron microscopy confirmed this result and permitted precise localization of the binding sites on the outermost fibrillar layer of the germ tube cell wall. B...

  6. The diploid genome sequence of Candida albicans

    OpenAIRE

    Jones, Ted; Federspiel, Nancy A.; Chibana, Hiroji; Dungan, Jan; Kalman, Sue; Magee, B. B.; Newport, George; Thorstenson, Yvonne R.; Agabian, Nina; Magee, P T; Davis, Ronald W.; Scherer, Stewart

    2004-01-01

    We present the diploid genome sequence of the fungal pathogen Candida albicans. Because C. albicans has no known haploid or homozygous form, sequencing was performed as a whole-genome shotgun of the heterozygous diploid genome in strain SC5314, a clinical isolate that is the parent of strains widely used for molecular analysis. We developed computational methods to assemble a diploid genome sequence in good agreement with available physical mapping data. We provide a whole-genome description ...

  7. Vacuolar trafficking and Candida albicans pathogenesis

    OpenAIRE

    Palmer, Glen E.

    2011-01-01

    The vacuole is likely to play a variety of roles in supporting host colonization and infection by pathogenic species of fungi. In the human pathogen Candida albicans, the vacuole undergoes dynamic morphological shifts during the production of the tissue invasive hyphal form, and this organelle is required for virulence. Recent efforts in my lab have focused on defining which vacuolar trafficking pathways are required for C. albicans hyphal growth and pathogenesis. Our results indicate that th...

  8. Milestones in Candida albicans Gene Manipulation

    OpenAIRE

    Samaranayake, Dhanushki P.; Hanes, Steven D.

    2011-01-01

    In the United States, candidemia is one of the most common hospital-acquired infections and is estimated to cause 10,000 deaths per year. The species Candida albicans is responsible for the majority of these cases. As C. albicans is capable of developing resistance against the currently available drugs, understanding the molecular basis of drug resistance, finding new cellular targets, and further understanding the overall mechanism of C. albicans pathogenesis are important goals. To study th...

  9. Candida famata-induced fulminating cholecystitis

    Directory of Open Access Journals (Sweden)

    Paulo Sergio Ramos de Araujo

    2013-12-01

    Full Text Available Lithiasic cholecystitis is classically associated with the presence of enterobacteria, such as Escherichia coli, Enterococcus, Klebsiella, and Enterobacter, in the gallbladder. Cholecystitis associated with fungal infections is a rare event related to underlying conditions such as diabetes mellitus, steroid use, and broad-spectrum antibiotic use for prolonged periods, as well as pancreatitis and surgery of the digestive tract. Here, we present the first reported case of a gallbladder infection caused by Candida famata.

  10. Triclosan antagonises fluconazole activity against Candida albicans

    OpenAIRE

    2012-01-01

    Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L) triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1? and cdr2? strains. Triclosan did not affect fluconazole uptake or alter total m...

  11. Triclosan Antagonizes Fluconazole Activity against Candida albicans

    OpenAIRE

    Higgins, J.; Pinjon, E.; Oltean, H.N.; White, T. C.; Kelly, S.L.; Martel, C.M.; Sullivan, D. J.; Coleman, D C; MORAN, G.P

    2012-01-01

    Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total ...

  12. Candida albicans Biofilm-Defective Mutants

    OpenAIRE

    Richard, Mathias L.; Nobile, Clarissa J.; Bruno, Vincent M; Mitchell, Aaron P.

    2005-01-01

    Biofilm formation plays a key role in the life cycles and subsistence of many microorganisms. For the human fungal pathogen Candida albicans, biofilm development is arguably a virulence trait, because medical implants that serve as biofilm substrates are significant risk factors for infection. The development of C. albicans biofilms in vitro proceeds through an early phase, in which yeast cells populate a substrate, an intermediate phase, in which pseudohyphal and hyphal cell types are produc...

  13. Mechanisms of Candida biofilm drug resistance

    OpenAIRE

    Taff, Heather T.; Mitchell, Kaitlin F.; Edward, Jessica A; Andes, David R.

    2013-01-01

    Candida commonly adheres to implanted medical devices, growing as a resilient biofilm capable of withstanding extraordinarily high antifungal concentrations. As currently available antifungals have minimal activity against biofilms, new drugs to treat these recalcitrant infections are urgently needed. Recent investigations have begun to shed light on the mechanisms behind the profound resistance associated with the biofilm mode of growth. This resistance appears to be multifactorial, involvin...

  14. White-opaque switching in Candida albicans

    OpenAIRE

    Lohse, Matthew B.; Johnson, Alexander D.

    2009-01-01

    The human commensal yeast Candida albicans undergoes an epigenetic switch between two distinct types of cells, referred to as white and opaque. These two cell types differ in many respects, including their cell and colony morphologies, their metabolic states, their mating behaviors, their preferred niches in the host, and their interactions with the host immune system. Each of the two cell types is heritable for many generations and switching between them appears stochastic; however, environm...

  15. DIARE YANG DISEBABKAN OLEH CANDIDA PADA ANAK DI UJUNG PANDANG

    Directory of Open Access Journals (Sweden)

    Muhammad Asaad Maidin

    2012-09-01

    Full Text Available Candida spp were isolated from 18 out of 38 (47,4 % children under five years with acute diarrhoea and from 10 out of35 (28,6% children under five years without diarrhoea. Statistical tests showed no difference between the presence of Candida species and the occurence of diarrhoea of the two groups of children (p >0,05. Mycelia were isolated from one diarrhoeal patient (1/387 or 2,63%. From the diarrhoeal patients, the Candida species found, were Candida albicans 61,1% (11/18, Candida parapsilosis 5,6% (1/18, Candida pseudotropicalis 22,2%(4/18, and Candida tropicalis 11,1 %(2/18. While from the non diarrhoeal patients 30% (3/10 were Candida Albicans, 20% (2/10 were Can­dida pseudotropicalis, and 50%(5/10 were Candida tropicalis. Bacterial identification of rectal swabs from diarrhoeal patients showed that 2,63% (1/38 had Vibrio Cholera 2,63% had Shigella, 94,7% (36/38 had E. coli, 21,1% (8/38 had Proteus sp, 7,9% (3/38 had Providentia, and 2,6 % (1/38 had Arizona. The clinical symptoms of the diarrhoeal patients were 97,4% had watery diarrhoea, 63,2% de­fecated more than 10 times, and 78,9% had vomiting.

  16. Intestinal colonization with Candida albicans and mucosal immunity

    Institute of Scientific and Technical Information of China (English)

    Xiao-Dong Bai; Xian-Hua Liu; Qing-Ying Tong

    2004-01-01

    AIM: To observe the relationship between intestinal lumen colonization with Candida albicans and mucosal secretory IgA (sIgA).METHODS: A total of 82 specific-pathogen-free mice were divided randomly into control and colonization groups. After Candida albicans were inoculated into specific-pathogenfree mice, the number of Candida albicans adhering to cecum and mucosal membrane was counted. The lymphocyte proliferation in Peyer's patch and in lamina propria was shown by BrdU incorporation, while mucosal sIgA (surface membrane) isotype switch in Peyer's patch was investigated. IgA plasma cells in lamina propria were observed by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.RESULTS: From d 3 to d 14 after Candida albicans gavaging to mice, the number of Candida albicans colonizing in lumen and adhering to mucosal membrane was sharply reduced.Candida albicans translocation to mesenteric lymph nodes occurred at early time points following gavage administration and disappeared at later time points. Meanwhile, the content of specific IgA was increased obviously. Proliferation and differentiation of lymphocytes in lamina propria were also increased.CONCLUSION: Lymphocytes in lamina propria play an important role in intestinal mucosal immunity of specificpathogen-free mice when they are first inoculated with Candida albicans. The decreasing number of Candida albicans in intestine is related to the increased level of specific IgA antibodies in the intestinal mucus.

  17. Biosorption of 241Am by Candida sp

    International Nuclear Information System (INIS)

    The biosorption of radionuclide 241Am from solutions by Candida sp., and the influences of experimental conditions on the adsorption were studied. The results showed that the adsorption equilibrium was achieved within 4h and the optimum pH=2. No significant differences on 241Am biosorption were observed at 10-45 degree C, or challenged with Au3+ or Ag+, even 1500 times or 4500 times over 241Am, respectively. The adsorption rate could reach 97.8% by dry Candida sp. of 0.82 g/L in 241Am solutions (pH=2) of 5.6-111 MBq/L (44.04-873.0 μg/L) (C0), with maximum adsorption capacity (W) of 63.5 MBq/g (501.8 μg/g), implying that the removal of 241Am by Candida sp. from solutions was feasible. The relationship between activities (C0) and adsorption capacities (W) of 241Am indicated that the biosorption process could be described by Langmuir adsorption isotherm

  18. Endogenous Candida endophthalmitis after induced abortion.

    Science.gov (United States)

    Chen, S J; Chung, Y M; Liu, J H

    1998-06-01

    Reported, in this article, are the cases of two young women who developed endogenous Candida endophthalmitis after induced abortion. Both women experienced transient fever, chills, and abdominal pain after the abortion and were given antibiotics. The diagnosis of endophthalmitis was established on the basis of typical fundus appearance, positive vaginal culture, and (in one case) positive vitreous culture. In the first woman, who received vitrectomy and intravitreal amphotericin B injection, the affected eye had a best corrected visual acuity of 20/200. In the second woman, who was given systemic corticosteroid treatment before the correct diagnosis was reached, recurrent retinal detachment developed and the best corrected visual acuity was counting fingers. It appears that Candida organisms harbored in the genital tract are directly inoculated into the venous system during induced abortion. Once in the blood, if sufficient fungal load is present, Candida albicans tends to localize in the choroid and to spread toward the retina and vitreous cavity. The immunosuppressive effect of corticosteroids further increases the risk of endophthalmitis. PMID:9645729

  19. Ocronose exógena induzida por hidroquinona: relato de quatro casos Exogenous ochronosis hydroquinone induced: a report of four cases

    OpenAIRE

    Jonas Ribas; Antonio Pedro Mendes Schettini; Melissa de Sousa Melo Cavalcante

    2010-01-01

    A ocronose exógena é uma dermatose, aparentemente pouco frequente, caracterizada por hiperpigmentação negro-azulada fuliginosa, localizada na região onde foi aplicado o agente causador. Pode ser causada por uso de medicamentos sistêmicos, os antimaláricos e de uso tópico, como fenol, resorcinol, benzeno, ácido pícrico e a hidroquinona - que é um composto fenólico, com propriedade despigmentante, muito utilizado em formulações dermatológicas para o tratamento de melasma e outras hiperpigmentaç...

  20. Edema pulmonar por presión negativa: a propósito de 3 casos Negative pressure pulmonary edema: 3 cases report

    OpenAIRE

    J.R. Ortiz-Gómez; I. Plaja-Marti; F. Sos-Ortigosa; J.J. Pérez-Cajaraville; M. A. Arteche-Andrés; Bengoechea, C.; J. Lobo-Palanco; M. Ahmad-Al-Ghool

    2006-01-01

    El edema agudo pulmonar por presión negativa es una complicación descrita desde 1977 tras la obstrucción de la vía aérea respiratoria, tanto en niños como en adultos. Aunque su etiopatogenia es multifactorial, destaca especialmente la excesiva presión intratorácica negativa causada por la inspiración forzada espontánea de un paciente con la glotis cerrada, que resulta en trasudación de líquido de los capilares pulmonares hacia el espacio alveolointersticial. El edema pulmonar resultante puede...

  1. Histopatologia da leishmaniose tegumentar por Leishmania braziliensis braziliensis: 1. Padrões histopatológicos e estudo evolutivo das lesões

    OpenAIRE

    Albino Verbosa de Magalhães; Mario A. P. Moraes; Alberto N. Raick; Alejandro Llanos-Cuentas; Jackson M. L. Costa; Cesar C. Cuba; Philip D. Marsden

    1986-01-01

    Os autores analisam material de biópsias de 378 casos de Leishmaniose Tegumentar, causada por Leishmania braziliensis braziliensis, da localidade endêmica de Três Braços (Estado da Bahia). O parásitos, embora escassos, foram encontrados em 63,7% dos casos da forma cutânea e em 37,5% dos casos da forma mucosa. As alterações dérmicas ou do córion da mucosa permitiram identificar cinco padrões histopatológicos: 1) Reação Exsudativa Celular, constituída por um infiltrado histiolinfoplasmocitário;...

  2. Efecto de plantas usadas etnomédicamente sobre la actividad hemorrágica y proteolítica inducida por Bothrops asper

    OpenAIRE

    Beatriz Badilla Baltodano; Fernando Chaves Mora; Luis Jorge Poveda Álvarez; Sandra Jiménez Castro; Gina Rodríguez Rodríguez

    2006-01-01

    Se evaluó la capacidad para neutralizar la acción hemorrágica y proteolítica causada por el veneno de la serpiente Bothrops asper de extractos acuosos de 5 plantas utilizadas etnomédicamente para tratar este problema. Este trabajo forma parte de un estudio de tamizaje farmacológico sobre las plantas usadas por los curanderos para tratar las mordeduras de serpientes. Se usaron extractos liofilizados de las hojas de Buddleja americana H.B.& K. (Buddlejaceae), Mikania guaco Humb. & Bonpl. (Aster...

  3. Quantificação da floculação de Saccharomyces cerevisiae por bactérias contaminantes da fermentação alcoólica

    OpenAIRE

    LUDWIG K.M.; OLIVA-NETO P.; D. F. Angelis

    2001-01-01

    O assentamento de células de leveduras no fundo das dornas e perdas de células nas centrífugas podem ser causadas por bactérias floculantes, contaminantes naturais da fermentação alcoólica industrial. Estes problemas levam a queda no rendimento e produtividade do etanol. O presente trabalho visa a caracterização da floculação de Saccharomyces cerevisiae por Lactobacillus fermentum CCT 1396. As células de leveduras e bactérias foram misturadas e a floculação das células quantificadas por espec...

  4. Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida

    OpenAIRE

    Horvath Lynn L; Floyd Karon L; Beckius Miriam L; Hospenthal Duane R; Murray Clinton K

    2006-01-01

    Abstract Background CHROMagar Candida (CaC) is increasingly being reported as a medium used to differentiate Candida albicans from non-albicans Candida (NAC) species. Rapid identification of NAC can assist the clinician in selecting appropriate antifungal therapy. CaC is a differential chromogenic medium designed to identify C. albicans, C. krusei, and C. tropicalis based on colony color and morphology. Some reports have proposed that CaC can also reliably identify C. dubliniensis and C. glab...

  5. Performance comparison of phenotypic and molecular methods for detection and differentiation of Candida albicans and Candida dubliniensis

    OpenAIRE

    Ahmad Suhail; Khan Ziauddin; Asadzadeh Mohammad; Theyyathel Ajmal; Chandy Rachel

    2012-01-01

    Abstract Background Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, however, C. dubliniensis has the propensity to develop resistance against azoles more easily. W...

  6. Comparison of cell wall proteins in putative Candida albicans & Candida dubliniensis by using modified staining method & SDS-PAGE

    OpenAIRE

    Yazdanparast, Seyed Amir; Nezarati, Seyedeh Shahrzad Mahdavi; Heshmati, Fariba; Hamzehlou, Sepideh

    2012-01-01

    Background Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C. albicans is very polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they enable yeast to adhere to host cells and begin pathogenesis. Therefore, we decided to extract these prot...

  7. Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts

    OpenAIRE

    Kozik, Andrzej; Karkowska-Kuleta, Justyna; Zajac, Dorota; Bochenska, Oliwia; Kedracka-Krok, Sylwia; Jankowska, Urszula; Rapala-Kozik, Maria

    2015-01-01

    Background Candida parapsilosis and C. tropicalis increasingly compete with C. albicans—the most common fungal pathogen in humans—as causative agents of severe candidiasis in immunocompromised patients. In contrast to C. albicans, the pathogenic mechanisms of these two non-albicans Candida species are poorly understood. Adhesion of Candida yeast to host cells and the extracellular matrix is critical for fungal invasion of hosts. Methods The fungal proteins involved in interactions with extrac...

  8. Fagoterapia frente a infecciones por Staphylococcus aureus meticilino resistente en ratones

    Directory of Open Access Journals (Sweden)

    Jesús H Tamariz

    2014-03-01

    Full Text Available Objetivos: Evaluar la actividad de los bacteriófagos frente a infecciones localizadas y sistémicas producidas por Staphylococcus aureus resistente a meticilina (MRSA Materiales y métodos. Se realizó un estudio de tipo experimental en 45 ratones de la cepa Balb/c divididos en nueve grupos de cinco individuos. Se aislaron diez bacteriófagos nativos a partir de muestras clínicas y efluentes hospitalarios, se evaluó su capacidad lítica y su espectro de actividad, en base a lo cual se seleccionaron seis fagos para los ensayos de fagoterapia. Adicionalmente, se empleó un bacteriófago de origen comercial. La fagoterapia fue evaluada mediante profilaxis y terapia de infecciones localizadas y sistémicas causadas por la inoculación de MRSA por vía subcutánea y endovenosa respectivamente. Se probó la efectividad de tres esquemas terapéuticos: monoterapia, cóctel de fagos en múltiples dosis y de cóctel de fagos en una sola dosis. También se comparó la actividad terapéutica de los fagos frente a vancomicina y clindamicina. Resultados. El cóctel de fagos y la terapia a diversas dosis fueron efectivos para prevenir y controlar infecciones localizadas por MRSA, su actividad fue similar a la de vancomicina y clindamicina. La dosis única del cóctel de fagos no logró controlar la infección localizada; asimismo, la fagoterapia no resultó efectiva en infecciones sistémicas. Conclusiones. La fagoterapia se proyecta como una alternativa viable frente a infecciones causadas por MRSA. Se requieren estudios que evalúen aspectos relacionados con la inocuidad de los fagos frente al paciente

  9. Fagoterapia frente a infecciones por Staphylococcus aureus meticilino resistente en ratones

    Directory of Open Access Journals (Sweden)

    Jesús H Tamariz

    2014-01-01

    Full Text Available Objetivos: Evaluar la actividad de los bacteriófagos frente a infecciones localizadas y sistémicas producidas por Staphylococcus aureus resistente a meticilina (MRSA Materiales y métodos. Se realizó un estudio de tipo experimental en 45 ratones de la cepa Balb/c divididos en nueve grupos de cinco individuos. Se aislaron diez bacteriófagos nativos a partir de muestras clínicas y efluentes hospitalarios, se evaluó su capacidad lítica y su espectro de actividad, en base a lo cual se seleccionaron seis fagos para los ensayos de fagoterapia. Adicionalmente, se empleó un bacteriófago de origen comercial. La fagoterapia fue evaluada mediante profilaxis y terapia de infecciones localizadas y sistémicas causadas por la inoculación de MRSA por vía subcutánea y endovenosa respectivamente. Se probó la efectividad de tres esquemas terapéuticos: monoterapia, cóctel de fagos en múltiples dosis y de cóctel de fagos en una sola dosis. También se comparó la actividad terapéutica de los fagos frente a vancomicina y clindamicina. Resultados. El cóctel de fagos y la terapia a diversas dosis fueron efectivos para prevenir y controlar infecciones localizadas por MRSA, su actividad fue similar a la de vancomicina y clindamicina. La dosis única del cóctel de fagos no logró controlar la infección localizada; asimismo, la fagoterapia no resultó efectiva en infecciones sistémicas. Conclusiones. La fagoterapia se proyecta como una alternativa viable frente a infecciones causadas por MRSA. Se requieren estudios que evalúen aspectos relacionados con la inocuidad de los fagos frente al paciente

  10. La responsabilidad del Estado por el hecho del legislador

    Directory of Open Access Journals (Sweden)

    María Consuelo Alonso

    2011-12-01

    Full Text Available Analizar el fenómeno de la responsabilidad del Estado causada por los hechos del legislador ha sido un trabajo desarrollado tanto por la doctrina como por la jurisprudencia internacional a mediados del siglo xx. En Francia, por ejemplo, se presentó el primer antecedentejurisprudencial en el año de 1934. En España, su estudio empezó a gestionarse con la promulgación de la Constitución de 1978, dándose el primer fallo judicial en el año de 1993. Para el caso colombiano, la Constitución de 1991 introdujo en su artículo 90 el fundamento constitucional de la responsabilidad patrimonial del Estado, sinque esto significase que la jurisprudencia del Consejo de Estado encontrase las bases de esta responsabilidad en diferentes disposiciones de la Constitución de 1886, tales como los artículos 2º, 16 y 30, que consagró el principio de legalidad del Estado para proteger la vida, honra y bienes de los ciudadanos, y garantizar la propiedad privada y los demás derechos adquiridos con el título de derecho. Sin embargo, los primeros fallos relacionados con este título de imputación en Colombia fueron emitidos por el máximo Tribunal de lo Contencioso Administrativo colombiano en el año de 1998 y por la Corte Constitucional a través de la Sentencia C-038 de 2006, providencia judicial que estableció algunas de las características que posee esta figura jurídica.

  11. Evaluation of Candida Colonization and Specific Humoral Responses against Candida albicans in Patients with Atopic Dermatitis

    OpenAIRE

    Ghaffari Javad; Mehdi Taheri Sarvtin; Mohammad Taghi Hedayati; Zohreh Hajheydari; Jamshid Yazdani; Tahereh Shokohi 2

    2015-01-01

    The aim of this study was to assess the candidal colonization and specific humoral responses against Candida albicans in patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagar Candida medium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodie...

  12. Diagnostico por PCR del complejo Sigatoka en Colombia.

    Directory of Open Access Journals (Sweden)

    Romero Magally

    1999-06-01

    Full Text Available Las Sigatokas negra y amarilla son enfermedades causadas por Mycosphaerella fijiensis y Mycosphaerella musicola respectivamente. Estas dos especies de hongos estrechamente relacionadas y morfológicamente similares, causan necrosis severa en las hojas de banano y plátano disminuyendo el área de tejido fotosintético y acelerando la maduración del fruto. Las enfermedades producidas por estos hongos afectan la mayoría de las áreas cultivadas de banano y plátano a nivel mundial, generando un gran problema económico y ambiental. Los síntomas que produce cada uno de estos patógenos en el cultivo son similares, por lo que su correcta identificación en campo se hace difícil. Con el fin de hacer una identificación precisa de cada uno de ellos decidimos estandarizar una prueba diagnóstica basada en la reacción en cadena de la polimerasa (PCR utilizando dos oligonucleótidos de 21 bases (MF 137 y MM 137 específicos para cada una de las especies M. fijiensis y M. musicola respectivamente. MF 137 y MM 137 codifican regiones variables identificadas en las secuencias interespaciadoras (ITS del DNA ribosomal.

  13. Influence of probiotics on Candida presence and IgA anti-Candida in the oral cavity

    Directory of Open Access Journals (Sweden)

    Agda Lima dos Santos

    2009-12-01

    Full Text Available Probiotics are defined as microorganisms that promote benefits to host health, mainly by regulating resident microbiota. Disequilibrium in microbiota can favor the growth of opportunist microorganisms and the development of pathologies, like candidosis caused by yeasts of the Candida genus. This work evaluated whether probiotics consumption was able to influence a specific immunological response to Candida and the presence of these yeasts in the oral cavity. Saliva samples were collected from healthy individuals and plated in Dextrose Saboraud Agar with chloramphenicol. Individuals presenting Candida in the oral cavity used the probiotic Yakult LBâ for 20 days, after which new collections and identifications were performed. Anti-Candida IgA analysis was conducted using the ELISA technique. Analysis of the results showed a significant reduction in Candida prevalence (46% and mean Candida CFU/mL counts (65%. The Candida species identified were C. albicans (98% and C.tropicalis (2%, before and after probiotics consumption. Immunological analysis demonstrated a significant reduction in anti-Candida IgA levels after probiotics use, probably due to less antigenic stimulation. In conclusion, in the individuals studied, probiotics use significantly reduced the amount of Candida in the oral cavity, possibly due to competition between the yeasts rather than by specific secretory immune response stimulation.

  14. Compactação causada pelo tráfego de trator em diferentes manejos de solo = Soil compaction induced by tractor traffic in different soil managements

    Directory of Open Access Journals (Sweden)

    João Paulo Arantes Rodrigues da Cunha

    2009-07-01

    Full Text Available O objetivo deste trabalho foi avaliar a compactação do solo causada pelo tráfego de trator, em um Latossolo Vermelho distroférrico submetido a diferentes tipos de preparo. O delineamento experimental utilizado foi o de blocos casualizados, com arranjo de parcelassubdivididas, considerando os tipos de preparo de solo como tratamentos principais e o número de passadas do trator como tratamentos secundários. Como tratamentos de preparo de solo, além de uma testemunha sem movimentação, foram avaliados: aração com 18 cmde profundidade, aração com 18 cm de profundidade, seguida de gradagem a 10 cm e subsolagem a 40 cm de profundidade. Após o preparo, foi avaliada a resistência do solo à penetração, por meio de um penetrômetro eletrônico, antes da passagem do trator e apósuma, três, cinco e sete vezes a passagem do trator na mesma linha de tráfego. Também se realizou a avaliação de densidade do solo. Concluiu-se que quanto maior a pulverização do solo, maior foi o potencial de compactação. Solos recentemente arados e gradeados forammais susceptíveis à compactação do que solos somente arados ou subsolados. O efeito do tráfego do trator concentrou-se principalmente na camada superficial, e a primeira passada foi a que mais provocou compactação do solo.The objective of this work was to evaluate soil compaction induced bytractor traffic in a Red Latosol (Typic Acrustox, submitted to different soil managements. The experiment was arranged in a subdivided parcel scheme, with types of soil tillage as theprimary soil treatments and the number of tractor passes as secondary treatments. In addition to a control treatment without soil tillage, the treatments were: tilling to a depth of 18 cm, a tilling to a depth of 18 cm followed by harrow use at a depth of 10 cm, and a 40-cm deep subsoil tilling. After preparing the parcels, soil penetration resistance was measured with an electronic penetrometer, before tractor passes and

  15. Isolation Frequency Characteristics of Candida Species from Clinical Specimens

    Science.gov (United States)

    Kim, Ga-Yeon; Jeon, Jae-Sik

    2016-01-01

    Candida spp. is an invasive infectious fungus, a major risk factor that can increase morbidity and mortality in hospitalized patients. In this study, 2,508 Candida spp. were isolated from various clinical specimens collected from university hospitals from July 2011 to October 2014. They were identified in order to determine isolation frequencies and characteristics by specimen, gender, age group, year, season, and month. The strain-specific isolation rate of Candida spp. is in the order of Candida albicans (1,218 strains, 48.56%), Candida glabrata (416 strains, 16.59%), Candida utilis (305 strains, 12.16%), Candida tropicalis (304 strains, 12.12%), and Candida parapsilosis (116 strains, 4.63%) and these five species accounted for more than 94% of the total strains. Of the specimens, Candida spp. were most frequently isolated from urine-catheter, followed by urine-voided, blood, sputum, other, open pus, vaginal discharge, Tip, ear discharge, bronchial aspiration and bile, in that order. Looking at the age distribution, the detection rate of patients in their 60s and older was significantly higher at 75.8% (1,900/2,508). The detection rate of patients in their 20s and younger was shown to be very low at 2.55% (64/2,508). By year, the detection rate of non-albicans Candida spp. showed a tendency to gradually increase each year compared with C. albicans. As isolation of Candida spp. from clinical samples at the specie level can vary depending on characteristics of the patient, sample, season, etc., continual studies are required. PMID:27433120

  16. Terapia fotodinámica in vitro con Hipericina en hongos causantes de micosis cutáneas

    OpenAIRE

    Paz Cristóbal, Manuel Pablo de la; Pardo Jimeno, Julián; Gilaberte Calzada, Yolanda; Rezusta López, Antonio

    2013-01-01

    Las infecciones cutáneas o micosis son muy frecuentes, especialmente las causadas por bacterias y hongos. Dentro de las del segundo tipo, denominadas micosis, se encuentran las producidas por hongos dermatofitos o las causadas por levaduras, especialmente por Candida spp. (15). Un uso abusivo de las sustancias antibióticas clásicas, junto con la inapropiada prescripción médica promueven la generación de resistencias de estos microorganismos frente a los agentes antifúngicos. Además, en los úl...

  17. Linfadenitis intratorácica, falla respiratoria y muerte por tuberculosis Fatal respiratory failure due to tuberculous intrathoracic lymphadenitis

    OpenAIRE

    Lazaro Vélez

    1989-01-01

    La Iinfadenitis tuberculosa del adulto afecta los ganglios intratorácicos sólo en 5-7% de los casos y generalmente produce poco compromiso sistémico. Se presenta el caso de una mujer de 21 anos que murió en Insuficiencia respiratoria debida a la obstrucción bronquial causada por grandes adenopatias hiliares y mediastinales y derrame pleural masi...

  18. Candida Arthritis: Analysis of 112 Pediatric and Adult Cases.

    Science.gov (United States)

    Gamaletsou, Maria N; Rammaert, Blandine; Bueno, Marimelle A; Sipsas, Nikolaos V; Moriyama, Brad; Kontoyiannis, Dimitrios P; Roilides, Emmanuel; Zeller, Valerie; Taj-Aldeen, Saad J; Miller, Andy O; Petraitiene, Ruta; Lortholary, Olivier; Walsh, Thomas J

    2016-01-01

    Background.  Candida arthritis is a debilitating form of deeply invasive candidiasis. However, its epidemiology, clinical manifestations, management, and outcome are not well understood. Methods.  Cases of Candida arthritis were reviewed from 1967 through 2014. Variables included Candida spp in joint and/or adjacent bone, underlying conditions, clinical manifestations, inflammatory biomarkers, diagnostic imaging, management, and outcome. Results.  Among 112 evaluable cases, 62% were males and 36% were pediatric. Median age was 40 years (range, osteomyelitis was present in 30% of cases. Candida albicans constituted 63%, Candida tropicalis 14%, and Candida parapsilosis 11%. Most cases (66%) arose de novo, whereas 34% emerged during antifungal therapy. Osteolysis occurred in 42%, joint-effusion in 31%, and soft tissue extension in 21%. Amphotericin and fluconazole were the most commonly used agents. Surgical interventions included debridement in 25%, irrigation 10%, and drainage 12%. Complete or partial response was achieved in 96% and relapse in 16%. Conclusion.  Candida arthritis mainly emerges as a de novo infection in usually non-immunosuppressed patients with hips and knees being most commonly infected. Localizing symptoms are frequent, and the most common etiologic agents are C albicans, C tropicalis, and C parapsilosis. Management of Candida arthritis remains challenging with a clear risk of relapse, despite antifungal therapy. PMID:26858961

  19. Preparation of Candida albicans Biofilms for Transmission Electron Microscopy

    OpenAIRE

    Taff, Heather T.; Andes, David R.

    2013-01-01

    Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For Candida albicans biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix Candida albicans biofilms in preparation for Transmission Electron Microscopy.

  20. Genetic Relationship between Human and Animal Isolates of Candida albicans

    OpenAIRE

    Edelmann, Anke; Krüger, Monika; SCHMID, JAN

    2005-01-01

    Analyzing Candida albicans isolates from different human and animal individuals by Ca3 fingerprinting, we obtained no evidence for host-specific genotypes and for the existence of species-specific lineages, even though a certain degree of separation between human and animal isolates was found. Therefore, animals could potentially serve as reservoirs for human Candida infection.

  1. A Rare Cause of Persistent Nausea and Vomiting: Candida Duodenitis

    Directory of Open Access Journals (Sweden)

    Mustafa Yildirim

    2014-03-01

    Full Text Available Ižntestinal bowel lesions caused by Candida species are uncommon. These lesions may be  plaques or ulcers form. In this article, We presented endoscopic images  Candida duodenitis as a cause of persistent nausea, vomiting in a lung cancer patient treated with chemotherapy.  

  2. A Rare Cause of Persistent Nausea and Vomiting: Candida Duodenitis

    OpenAIRE

    Mustafa Yildirim

    2014-01-01

    Intestinal bowel lesions caused by Candida species are uncommon. These lesions may be  plaques or ulcers form. In this article, We presented endoscopic images  Candida duodenitis as a cause of persistent nausea, vomiting in a lung cancer patient treated with chemotherapy.  

  3. Candida vaccines development from point view of US patent application.

    Science.gov (United States)

    Wang, Shyh-Jen

    2011-11-01

    Candidiasis is the fourth most common bloodstream infection in hospitalized patients in the United States. Moreover, the mortality rate from Candida infections remains high, even after treatment with antifungal therapy. Vaccination would be a promising strategy for prevention of invasive fungal infections. In order to examine the main trends in anticandidal vaccine patenting activity, we conducted an analysis for anticandidal vaccine patents. We find 190 issued patent and 940 patent application documents containing the keywords Candida and vaccine within claims in the USA. Candida vaccines development, as evidenced by the numbers of issued patents, has decreased since the year 2002. Furthermore, the number of patent applications in Candida vaccines may indicate the strength of engaged resources were also in the status of stagnation during 2005-2007 and even a decline in 2008. Academic and nonprofit research institutions not only account for a large share of Candida vaccines patents but also apply for patents continually. Based on this analysis, the strength of Candida vaccines resources seems to remain stagnant in recent years due to patent prosecution or technical barrier in the filed of Candida vaccines. Therefore, we consider that Candida vaccines technology to still be under development and the researchers are still looking for scientific breakthrough in the filed. PMID:22048114

  4. Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.

    OpenAIRE

    Fiss, E.; Buckley, H R

    1987-01-01

    Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. The C. albicans 48-kilodalton protein recognized by sera from patients with invasive candidiasis was shown by DEAE chromatography and immunoblotting not to be identical with the purified C. albicans actin.

  5. Molecular typing of Candida albicans strains isolated from nosocomial candidemia Tipificação molecular de espécies de Candida albicans isoladas de candidemia hospitalar

    Directory of Open Access Journals (Sweden)

    Maria Luiza Moretti Branchini

    1995-12-01

    Full Text Available Yeasts of the genus Candida have been recognized as important microorganisms responsible for nosocomial fungemia. Six blood-stream and two intravenous central catheter C. albicans strains were isolated from eight patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed-field gel electrophoresis. Seven chromosomal DNA profiles were identified. Two patients showed isolates with the same profile, suggesting nosocomial transmission. Karyotyping of C. albicans revealed an excellent discriminatory power among the isolates and may therefore be useful in the study of nosocomial candidemia.Leveduras do gênero Candida têm sido reconhecidas como importantes causadoras de fungemias hospitalares. Foram estudados os DNA cromossômicos de oito cepas de C. albicans, obtidas de oito pacientes com fungemia hospitalar, por cariotipagem eletroforética através de "pulsed-field gel electrophoresis". As cepas foram obtidas pelo isolamento da levedura em seis hemoculturas e duas infecções relacionadas ao uso de cateter intra-venoso central. Foram identificados sete perfis de DNA cromossômico. Dois pacientes mostraram cepas com o mesmo perfil de DNA sugerindo transmissão nosocomial. A cariotipagem eletroforética revelou excelente capacidade discriminatória entre os isolados sendo útil no estudo das candidemias hospitalares.

  6. Oral candidiasis-adhesion of non-albicans Candida species

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    Bokor-Bratić Marija B.

    2008-01-01

    Full Text Available Oral candidiasis is an opportunistic infection caused primarily by Candida albicans. However, in recent years, species of non-albicans Candida have been implicated more frequently in mucosal infection. Candida species usually reside as commensal organisms and are part of normal oral microflora. Determining exactly how transformation from commensal to pathogen takes place and how it can be prevented is continuous challenge for clinical doctors. Candidal adherence to mucosal surfaces is considered as a critical initial step in the pathogenesis of oral candidiasis. Acrylic dentures, acting as reservoirs, play an important role in increasing the risk from Candida colonisation. Thus, this review discusses what is currently known about the adhesion of non-albicans Candida species of oral origin to buccal epithelial cells and denture acrylics.

  7. Candida albicans and Candida parapsilosis induce different T-cell responses in human peripheral blood mononuclear cells

    NARCIS (Netherlands)

    Toth, A.; Csonka, K.; Jacobs, C.; Vagvolgyi, C.; Nosanchuk, J.D.; Netea, M.G.; Gacser, A.

    2013-01-01

    Candida parapsilosis is the third most frequent cause of candidemia. Despite its clinical importance, little is known about the human immunological response to C. parapsilosis. In this study, we compared the cytokine responses evoked by Candida albicans and C. parapsilosis. C. parapsilosis-stimulate

  8. Pharmacotherapy of Candida Infections with Echinocandins

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    Ana Espinel-Ingroff

    2009-01-01

    Full Text Available The classic recommended antifungal agents for the treatment of invasive Candida infections were amphotericin B, a lipid formulation of amphotericin B and fluconazole in both neutropenic or nonneutropenic patients as either primary or alternative therapies. Voriconazole has been recommended when additional coverage for filamentous fungi is needed (e.g. neutropenic patients. More recently and based on well designed comparative clinical trials, the three echinocandins, caspofungin, anidulafungin and micafungin have been added as primary or alternative therapies especially for critically ill or neutropenic patients. In general, the echinocandins are most useful when patients have previously been exposed to an azole or are unstable.

  9. Melittin induces apoptotic features in Candida albicans

    International Nuclear Information System (INIS)

    Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.

  10. A Candida albicans PeptideAtlas

    OpenAIRE

    Vialas, Vital; Sun, Zhi; Loureiro y Penha, Carla Verónica; Carrascal, Montserrat; Abián, Joaquín; Monteoliva, Lucía; Deutsch, Eric W.; Aebersold, Ruedi; Moritz, Robert L.; Gil, Concha

    2014-01-01

    Candida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained coverage of 41% of the C. albicans open reading frame sequences (ORFs) in the database used for the se...

  11. Melittin induces apoptotic features in Candida albicans

    Energy Technology Data Exchange (ETDEWEB)

    Park, Cana [School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701 (Korea, Republic of); Lee, Dong Gun, E-mail: dglee222@knu.ac.kr [School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701 (Korea, Republic of)

    2010-03-26

    Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.

  12. Compactação causada pelo tráfego de trator em diferentes manejos de solo = Soil compaction induced by tractor traffic in different soil managements

    OpenAIRE

    João Paulo Arantes Rodrigues da Cunha; Vinicius Nery Cascão; Elton Fialho dos Reis

    2009-01-01

    O objetivo deste trabalho foi avaliar a compactação do solo causada pelo tráfego de trator, em um Latossolo Vermelho distroférrico submetido a diferentes tipos de preparo. O delineamento experimental utilizado foi o de blocos casualizados, com arranjo de parcelassubdivididas, considerando os tipos de preparo de solo como tratamentos principais e o número de passadas do trator como tratamentos secundários. Como tratamentos de preparo de solo, além de uma testemunha sem movimentação, foram aval...

  13. Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

    Directory of Open Access Journals (Sweden)

    Nadăş George Cosmin

    2014-06-01

    Full Text Available The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP. The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93% strains, followed by Candida krusei - 17 (29.31% strains, Candida famata - 10 (17.24% strains, Candida parapsilosis - five (8.62% strains, and Candida kefyr - four (6.89% strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

  14. Osteomalacia inducida por tumor: hemangiopericitoma rinosinusal Tumor-induced osteomalacia: rhinosinusal hemangiopericytoma

    Directory of Open Access Journals (Sweden)

    Enriqueta M. Serafini

    2013-02-01

    Full Text Available La osteomalacia inducida por tumor es una rara enfermedad del metabolismo óseo caracterizada por el aumento en la excreción de fosfato a nivel renal seguido de hipofosfatemia. Es causada por agentes fosfatúricos producidos por determinados tumores. La resección total del tumor resulta en la completa reversión de las anormalidades bioquímicas, la desaparición de las manifestaciones clínicas y los hallazgos en los estudios por imágenes. Presentamos el caso de un varón de 61 años con cuadro clínico y laboratorio compatibles con osteomalacia oncogénica inducida por tumor mesenquimático de localización rinosinusal. En nuestro caso el diagnóstico histológico correspondió a una neoplasia de tipo vascular: hemangiopericitoma.Tumor-induced osteomalacia is a rare disease of bone metabolism. The characteristic of this disease is an increase in phosphate excretion followed by hypophosphatemia, due to phosphaturic agents produced by different types of tumors. Tumor resection results in complete resolution of clinical, biochemical and radiological abnormalities. We present the case of a 61 year old man with signs, symptoms and laboratory findings consistent with oncogenic osteomalacia due to a rhino-sinusal mesenchymal tumor. The histological diagnosis showed a vascular neoplasm: hemangiopericytoma.

  15. Activity of Polyphenolic Compounds against Candida glabrata

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    Ricardo Salazar-Aranda

    2015-09-01

    Full Text Available Opportunistic mycoses increase the morbidity and mortality of immuno-compromised patients. Five Candida species have been shown to be responsible for 97% of worldwide cases of invasive candidiasis. Resistance of C. glabrata and C. krusei to azoles has been reported, and new, improved antifungal agents are needed. The current study was designed to evaluatethe activity of various polyphenolic compounds against Candida species. Antifungal activity was evaluated following the M27-A3 protocol of the Clinical and Laboratory Standards Institute, and antioxidant activity was determined using the DPPH assay. Myricetin and baicalein inhibited the growth of all species tested. This effect was strongest against C. glabrata, for which the minimum inhibitory concentration (MIC value was lower than that of fluconazole. The MIC values against C. glabrata for myricitrin, luteolin, quercetin, 3-hydroxyflavone, and fisetin were similar to that of fluconazole. The antioxidant activity of all compounds was confirmed, and polyphenolic compounds with antioxidant activity had the greatest activity against C. glabrata. The structure and position of their hydroxyl groups appear to influence their activity against C. glabrata.

  16. Candida Parapsilosis Arthritis Involving the Ankle in a Diabetes Patient: A Case Report

    International Nuclear Information System (INIS)

    Candida parapsilosis is a rare opportunistic fungal pathogen of the musculoskeletal region. Immune function of almost all patients is severely disturbed. Most reported cases of septic arthritis of joints by Candida involve the knee, especially Candida parapsilosis. To our knowledge, there has been only one case report of Candida parapsilosis involving the ankle presented on only plain radiography. We report a case of Candida parapsilosis arthritis involving the ankle in a diabetes patient which was shown on MR imaging.

  17. Candida Parapsilosis Arthritis Involving the Ankle in a Diabetes Patient: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Sung, Jin Kyeong; Chun, Kyung Ah [Dept. of Radiology, The Catholic University of Korea Uijeongbu St. Mary' s Hospital, Uijeongbu (Korea, Republic of)

    2011-06-15

    Candida parapsilosis is a rare opportunistic fungal pathogen of the musculoskeletal region. Immune function of almost all patients is severely disturbed. Most reported cases of septic arthritis of joints by Candida involve the knee, especially Candida parapsilosis. To our knowledge, there has been only one case report of Candida parapsilosis involving the ankle presented on only plain radiography. We report a case of Candida parapsilosis arthritis involving the ankle in a diabetes patient which was shown on MR imaging.

  18. Interactions of Candida albicans with host epithelial surfaces

    Directory of Open Access Journals (Sweden)

    David W. Williams

    2013-10-01

    Full Text Available Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. The organism is however, commonly encountered as a commensal in healthy individuals where it is a component of the normal microflora. The key determinant in the type of relationship that Candida has with its host is how it interacts with the epithelial surface it colonises. A delicate balance clearly exists between the potentially damaging effects of Candida virulence factors and the nature of the immune response elicited by the host. Frequently, it is changes in host factors that lead to Candida seemingly changing from a commensal to pathogenic existence. However, given the often reported heterogeneity in morphological and biochemical factors that exist between Candida species and indeed strains of C. albicans, it may also be the fact that colonising strains differ in the way they exploit resources to allow persistence at mucosal surfaces and as a consequence this too may affect the way Candida interacts with epithelial cells. The aim of this review is to provide an overview of some of the possible interactions that may occur between C. albicans and host epithelial surfaces that may in turn dictate whether Candida removal, its commensal persistence or infection follows.

  19. ISOLASI SPESIES CANDIDA DARI TINJA PENDERITA HIV/AIDS

    Directory of Open Access Journals (Sweden)

    Pudji K Sjarifuddin

    2002-12-01

    Full Text Available Candida is a saprophyte in the human respiratory tract, gastro intestinal tract and also in the debris under the nail. Inpatients with compromised immunity such as HIV-AIDS, Candida is able to cause infection, in this case oral candidosisor esophagitis. In this study fungi were isolated from the stools of HIV/AIDS patients. Samples consisting of 95diarrheic stools from HIV/AIDS patients were investigated for the yeast especially Candida spp. The stools were inoculated onto Sabouraud dextrose agar then the fungi were identified using morphological methods and Chromagarmedium. Yeast colonies were found in 71 (74,74% out of 95 samples from which Candida was 42 44,21%, Geotrichum 24 (25,26%, and mixed of Candida and Geotrichum 3 (3,16%, Rhodotorula and Trichosporon 1(1,05% each. Species of Candida were identified as C. albicans, C. tropicalis, C. krusei, C. guilliermondii, C. glabrata, C. lusitaniae and C. kefyr. Although Candida could be isolated from the diarrheic stools of HIV/AIDS patients but its role on the cause of diarrhea is still questionable.

  20. Prevalence of oral Candida in the first year of life.

    Science.gov (United States)

    Stecksén-Blicks, C; Granström, E; Silfverdal, S A; West, C E

    2015-09-01

    Colonisation of the gastrointestinal tract is influenced by primary microbial exposure and bioactive factors in breastmilk. The aim was to explore the prevalence of oral Candida in the first year of life in relation to selected exposures. Oral Candida was studied in 100 healthy infants at 4 and 8 weeks, 3, 6 and 12 months of age and related to delivery mode, birth weight, infant health and feeding, antibiotics, antimycotics, steroids and probiotics in mother and infant, living conditions, maternal smoking and infections The association between lactoferrin and antisecretory factor in breastmilk and maternal serum haemoglobin, transferrin, and ferritin levels in relation to oral Candida was also explored. About 11% to 15% of the infants had oral Candida at the respective age. Colonisation was fairly stable until 6 months of age. There was no conclusive impact of the investigated exposures at entry. Infants with a furry pet at home had a lower frequency of Candida at 3 months, (P < 0.05) whereas all but one colonised infant had older siblings at 12 months (P < 0.01). Lactoferrin in breastmilk was negatively associated with colonisation at 6 months of age. It is concluded that 11 to 15% had oral Candida. Exposure to furry pets and siblings impacted oral Candida. PMID:26214300

  1. Development of a membrane based detection of Candida albicans

    OpenAIRE

    Almeida, Catarina Guerreiro Silva de

    2015-01-01

    Tese de mestrado integrado em Engenharia Biomédica e Biofísica , apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2015 Candida é uma família de fungos, normalmente, presente na flora gastrointestinal, nos orgãos genitais, no sistema respiratório e na pele de pessoas saudáveis e, até determinada quantidade, não trazem nenhum risco. Apenas 17 espécies de Candida podem ser consideradas como patogénicas para o ser humano e, dentro deste grupo, Candida albicans é a esp...

  2. Candida flosculorum sp. nov. and Candida floris sp. nov., two yeast species associated with tropical flowers.

    Science.gov (United States)

    Rosa, Carlos A; Pagnocca, Fernando C; Lachance, Marc-André; Ruivo, Carla C C; Medeiros, Adriana O; Pimentel, Mariana R C; Fontenelle, Julio C R; Martins, Rogério P

    2007-12-01

    Two ascomycetous yeast species, Candida flosculorum sp. nov. and Candida floris sp. nov., were isolated from tropical flowers and their associated insects. C. flosculorum was isolated from flower bracts of Heliconia velloziana and Heliconia episcopalis (Heliconiaceae) collected from two Atlantic rain forest sites in Brazil. C. floris was isolated from flowers of Ipomoea sp. (Convolvulaceae) growing on the banks of the river Paraguai in the pantanal ecosystem in Brazil and from an adult of the stingless bee Trigona sp. and a flower of Merremia quinquefolia (Convolvulaceae) in Costa Rica. C. flosculorum belongs to the Metschnikowiaceae clade and C. floris belongs to the Starmerella clade. The type strain of C. flosculorum is UFMG-JL13(T) (=CBS 10566(T)=NRRL Y-48258(T)) and the type strain of C. floris is UWO(PS) 00-226.2(T) (=CBS 10593(T)=NRRL Y-48255(T)). PMID:18048759

  3. Sunflower seed husk agar: A new medium for the differentiation of Candida dubliniensis from Candida albicans

    Directory of Open Access Journals (Sweden)

    Khan Z

    2005-01-01

    Full Text Available A sunflower ( Helianthus annuus seed husk agar medium has been developed and evaluated for differentiation of Candida dubliniensis from Candida albicans on the basis of colony morphology and chlamydospore production. All C. dubliniensis isolates (n=40 produced rough colonies with hyphal fringes and abundant chlamydospores whereas 101 of 105 (96.2% C. albicans isolates produced smooth colonies with no evidence of chlamydospore production. Since this medium is free from oil droplets, chlamydospores can be examined with greater clarity by Dalmau plate technique. This medium provides a simple and cost-effective tool for the presumptive differentiation of C. dubliniensis from C. albicans and is particularly suited for clinical microbiology laboratories where biochemical or molecular methods for the differentiation of these two species are not available.

  4. Candida albicans versus Candida dubliniensis: Why Is C. albicans More Pathogenic?

    LENUS (Irish Health Repository)

    Moran, Gary P

    2012-01-01

    Candida albicans and Candida dubliniensis are highly related pathogenic yeast species. However, C. albicans is far more prevalent in human infection and has been shown to be more pathogenic in a wide range of infection models. Comparison of the genomes of the two species has revealed that they are very similar although there are some significant differences, largely due to the expansion of virulence-related gene families (e.g., ALS and SAP) in C. albicans, and increased levels of pseudogenisation in C. dubliniensis. Comparative global gene expression analyses have also been used to investigate differences in the ability of the two species to tolerate environmental stress and to produce hyphae, two traits that are likely to play a role in the lower virulence of C. dubliniensis. Taken together, these data suggest that C. dubliniensis is in the process of undergoing reductive evolution and may have become adapted for growth in a specialized anatomic niche.

  5. Three novel species of the anamorphic yeast genus Candida in the Candida intermedia clade found in Japan, Thailand and Taiwan.

    Science.gov (United States)

    Nakase, Takashi; Jindamorakot, Sasitorn; Am-In, Somit; Lee, Ching-Fu; Imanishi, Yumi; Limtong, Savitree

    2011-01-01

    Four strains of yeasts isolated in Japan, Thailand and Taiwan were found to represent three novel species of the genus Candida. The three species are located in a clade including Candida tsuchiyae, Candida thailandica and Candida akabanensis in a tree based on the D1/D2 domain sequences of the large subunit rRNA genes but clearly differentiated from these relative species. Three novel species are proposed for these strains, i. e., Candida berkhoutiae sp. nov., for strains ST-49(T) (=BCC 7749(T)=NBRC 106733(T)=CBS 11722(T)) isolated from insect frass in Thailand and SA13S01 (=NBRC 106053) isolated from soil in Taiwan, Candida ezoensis sp. nov., for strain Y07-1601-2(T) (=NBRC 105019(T)=CBS 11753(T)) isolated from forest soil in Japan, and Candida inulinophila sp. nov., for ST-369(T) (=BCC 15081(T)=NBRC 106735(T)=CBS 11725(T)) isolated from an unidentified wild mushroom from Thailand. PMID:21606608

  6. Evaluation of a rapid immunochromatographic assay for identification of Candida albicans and Candida dubliniensis.

    Science.gov (United States)

    Marot-Leblond, Agnes; Grimaud, Linda; David, Sandrine; Sullivan, Derek J; Coleman, David C; Ponton, Jose; Robert, Raymond

    2004-11-01

    Candida dubliniensis was first established as a novel yeast species in 1995. It is particularly associated with recurrent episodes of oral candidosis in human immunodeficiency virus (HIV)-infected patients, but it has also been detected at other anatomical sites and at a low incidence level in non-HIV-infected patients. It shares so many phenotypic characteristics with C. albicans that it is easily misidentified as such. No rapid, simple, and commercial test that allows differentiation between C. dubliniensis and C. albicans has been developed, until now. Accurate species identification requires the use of genotype-based techniques that are not routinely available in most clinical microbiology diagnostic laboratories. The present study was designed to evaluate the efficiency of a new test (the immunochromatographic membrane [ICM] albi-dubli test; SR2B, Avrille, France) to differentiate between C. albicans and C. dubliniensis. The organisms evaluated were strains whose identities had previously been confirmed by PCR tests and freshly isolated clinical strains and included 58 C. albicans isolates, 60 C. dubliniensis isolates, and 82 isolates belonging to other species of yeast. The ICM albi-dubli test is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies that recognize two unrelated epitopes expressed by both species or specific to only one species. The assay requires no complex instrumentation for analysis and can be recommended for routine use in clinical microbiology laboratories. Results are obtained within 2 h and 30 min and are easy to interpret. This evaluation demonstrated the good performance of this immunochromatographic test for C. albicans and C. dubliniensis isolated on Sabouraud dextrose agar, CHOROMagar Candida, and CandidaSelect, with sensitivities and specificities ranging from 93.1 to 100%. These parameters decreased, however, to 91.4% when the test was performed with yeast isolated

  7. Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis

    OpenAIRE

    Marot-Leblond, Agnes; Grimaud, Linda; David, Sandrine; Sullivan, Derek J.; Coleman, David C.; Ponton, Jose; Robert, Raymond

    2004-01-01

    Candida dubliniensis was first established as a novel yeast species in 1995. It is particularly associated with recurrent episodes of oral candidosis in human immunodeficiency virus (HIV)-infected patients, but it has also been detected at other anatomical sites and at a low incidence level in non-HIV-infected patients. It shares so many phenotypic characteristics with C. albicans that it is easily misidentified as such. No rapid, simple, and commercial test that allows differentiation betwee...

  8. Prevenção de injúrias causadas por glyphosate em soja RR por meio do uso de aminoácido Prevention of RR soybean injuries caused by exogenous supply of aminoacids

    OpenAIRE

    L.H.S. Zobiole; R.S. Oliveira Jr.; J. Constantin; D.F. Biffe

    2011-01-01

    A área de soja resistente ao glyphosate (RR) tem aumentado a cada ano. No entanto, essa expansão com a tecnologia da soja RR aumentou o uso de glyphosate significativamente, e muitos agricultores têm notado que alguns cultivares RR apresentam injúrias visuais logo após a aplicação do glyphosate em pós-emergência. Dessa forma, dois experimentos foram instalados em anos distintos, com diferentes objetivos: o primeiro, visando avaliar a influência do glyphosate na soja RR nas variáveis fotossint...

  9. Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media.

    LENUS (Irish Health Repository)

    Citiulo, Francesco

    2009-10-01

    Candida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are >30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.

  10. Global Transcriptome Sequencing Identifies Chlamydospore Specific Markers in Candida albicans and Candida dubliniensis

    LENUS (Irish Health Repository)

    Palige, Katja

    2013-04-15

    Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.

  11. Antifungigrama para comprovar o potencial de ação dos extratos vegetais hidroglicólicos sobre Candida sp. (Berkhout Etest to confirm the action potential of plant hydroglycol extracts on Candida sp. (Berkhout

    Directory of Open Access Journals (Sweden)

    E.A.V. Glehn

    2012-01-01

    Full Text Available A candidíase vaginal é uma doença causada, na maioria das vezes, pelo fungo do gênero Candida sp, que habita o trato gastrintestinal e geniturinário da espécie humana e pode tornar-se patogênico sob determinadas condições. A maioria dos indivíduos desenvolve defesas imunológicas que impedem a proliferação e desenvolvimento de candidíase localizada ou disseminada. Embora a causa exata do aumento de espécies não-albicans seja desconhecida, há evidências de que a própria terapia antifúngica possa estar contribuindo para o processo. Linhagens de C. glabrata são mais resistentes aos imidazólicos do que a C. albicans, sendo necessária uma concentração 10 vezes superior de miconazol para eliminar a C. glabrata quando comparada a C. albicans. Foi realizado um antifungigrama testando o potencial de ação de produtos vegetais sobre o fungo Candida sp. Foi observado que, ocorreu inibição do fungo no contato com os extratos hidroglicólicos das plantas Arctium lappa L., Calendula officinalis L., Stryphnodendron adstringens (Mart. Coville e Tabebuia avellanedae Lorentz ex Griseb. A importância deste trabalho reside na possibilidade de desenvolvimento de tratamento complementar, menos agressivo, de menor custo e sem toxidade, o que possibilitaria melhor qualidade de vida para portadoras de candidíase vaginal recorrente ou não.Vaginal candidiasis is a disease caused, in most cases, by the fungus of the genus Candida sp., which inhabits the gastrointestinal and genitourinary tracts of the human species and can become pathogenic under certain conditions. Most individuals develop immune defenses that prevent the proliferation and the development of localized or disseminated candidiasis. Although the exact cause of the increase in non-albicans species is unknown, there is evidence that antifungal therapy itself may have contributed to it. Strains of C. glabrata are more resistant to imidazole than C. albicans, and a 10-fold higher

  12. Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity Diferenças na produção de exoenzimas e habilidade de aderência entre isolados de espécies de Candida provenientes do cateter, sangue e cavidade bucal

    Directory of Open Access Journals (Sweden)

    Carolina Rodrigues Costa

    2010-06-01

    Full Text Available Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.A produção de proteinase e fosfolipase e habilidade de adesão à célula epitelial bucal de 112 isolados de Candida originadas da cavidade bucal de pacientes infectados pelo HIV e de sangue e cateter de pacientes hospitalizados foram investigados. A produção de proteinase foi detectada por inoculação em ágar soro albumina bovina e a atividade de fosfolipase foi realizada usando emulsão de gema de ovo. A suspensão de levedura de cada isolado foi incubada com célula epitelial e o número de leveduras aderidas a c

  13. Treinamento resistido controla a pressão arterial de ratos hipertensos induzidos por l-NAME Resistance training controls arterial blood pressure in rats with L-NAME- induced hypertension

    OpenAIRE

    Ayslan Jorge Santos de Araujo; Anne Carolline Veríssimo dos Santos; Karine dos Santos Souza; Marlúcia Bastos Aires; Valter Joviniano Santana-Filho; Emerson Ticona Fioretto; Marcelo Mendonça Mota; Márcio Roberto Viana Santos

    2013-01-01

    FUNDAMENTO: A hipertensão arterial é uma síndrome multifatorial, crônica, causada tanto por fatores congênitos ou adquiridos. OBJETIVO: Avaliar os efeitos do treinamento físico resistido (TR) sobre pressão arterial, reatividade e morfologia vascular de ratos hipertensos induzidos por L-NAME. MÉTODOS: Ratos Wistar machos (200-250 g) foram divididos em 3 grupos: normotenso sedentário (NS), hipertenso sedentário (HS) e hipertenso treinado (HT). A hipertensão foi induzida pela administração de L-...

  14. Relación entre ruido por carga vehicular, molestia y atención escolar en estudiantes de nivel básico de la ciudad de Toluca 2004

    Directory of Open Access Journals (Sweden)

    Martha Elva Campuzano González

    2010-01-01

    Full Text Available Dos escuelas públicas de la ciudad de Toluca con diferente afluencia vehicular fueron seleccionadas para el estudio. Se realizaron medidas internas y externas de los niveles de ruido; los niveles de ruido encontrados sobrepasan los recomendados por la oms. Se aplicaron dos test de atención a los alumnos de las escuelas. El nivel de atención fue mejor para la escuela con menos tránsito. En el personal de ambas escuelas hubo molestia causada por el ruido vehicular.

  15. Relación entre ruido por carga vehicular, molestia y atención escolar en estudiantes de nivel básico de la ciudad de Toluca 2004

    OpenAIRE

    Martha Elva Campuzano González; Lilia Patricia Bustamante Montes; Miguel Angel Karam Calderón; Ninfa Ramírez Durán

    2010-01-01

    Dos escuelas públicas de la ciudad de Toluca con diferente afluencia vehicular fueron seleccionadas para el estudio. Se realizaron medidas internas y externas de los niveles de ruido; los niveles de ruido encontrados sobrepasan los recomendados por la oms. Se aplicaron dos test de atención a los alumnos de las escuelas. El nivel de atención fue mejor para la escuela con menos tránsito. En el personal de ambas escuelas hubo molestia causada por el ruido vehicular.

  16. Efectos de la administración de un inhibidor de la recaptación de serotonina, Sertralina, sobre los cambios inducidos por el estrés prenatal

    OpenAIRE

    Figueiredo Pereira de, Inês

    2016-01-01

    [ES] En las postrimerías del siglo pasado, se constató una vez más que las alteraciones causadas por estrés y ansiedad en la sociedad moderna, se encuentran entre los problemas de salud más comunes. Según la Organización Mundial de Salud (OMS), al menos el 25% de los adultos sufrirá alguna forma de trastornos por ansiedad en algún momento de su vida (Gordon y Hen, 2004; Anacker y cols., 2010), siendo las mujeres más susceptibles a este tipo de trastornos (Olff y cols., 2007). ...

  17. Intoxicação exógena por “chumbinho” como forma de autoextermínio no Estado de Goiás, 2003 - 2007

    OpenAIRE

    Anna Carolina Sousa da Silva; Fábio Paulo Vilela; Graciela Mara Ordones do Nascimento Brandão

    2010-01-01

    A intoxicação exógena por chumbinho é considerada importante causa de morbidade e mortalidade no Brasil devido ao fácil acesso, baixo custo e por sua eficácia frente às tentativas de suicídio, tornando-se um problema de Saúde Pública. No Estado de Goiás não existem levantamentos sobre esse tipo de intoxicação, causadas intencionalmente, designando esta investigação. Trata-se de um estudo descritivo, retrospectivo documental com abordagem quantitativa, com o objetivo de estabelecer o perfil da...

  18. Pansinusitis y afectación intracraneal por implante dental Pansinusitis and intracranial impact of a dental implant

    Directory of Open Access Journals (Sweden)

    Josep Rubio-Palau

    2012-03-01

    Full Text Available Las sinusitis odontógenas son una patología relativamente frecuente causada por infecciones dentales, quistes periapicales así como tras procedimientos bucodentales como una endodoncia, una elevación sinusal o la colocación de un implante. A continuación se presenta un caso extremo de una pansinusitis derecha con fistulización a espacio epidural causada por un implante osteointegrado. Ante la sospecha de una sinusitis maxilar de origen odontogénico se debe iniciar rápidamente un tratamiento antibiótico correcto y un seguimiento estrecho ya que pueden tener consecuencias fatales como la pérdida de un ojo, abscesos cerebrales o incluso la muerte.Odontogenic sinusitis is a relatively common disease caused by dental infections, periapical cysts and oral procedures such as root canal, sinus lift or implant placement. We report an extreme case of a right pansinusitis with an epidural space fistula caused by osseointegrated implants. When maxillary sinusitis of odontogenic origin is suspected, we should quickly start effective antibiotic treatment and monitor the patient closely because odontogenic sinusitis can have serious consequences, such as the loss of an eye, brain abscess or death.

  19. Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species

    Science.gov (United States)

    Zhang, Jing; Hung, Guo-Chiuan; Nagamine, Kenjiro; Li, Bingjie; Tsai, Shien; Lo, Shyh-Ching

    2016-01-01

    Culture-based identification methods have been the gold standard for the diagnosis of fungal infection. Currently, molecular technologies such as real-time PCR assays with short turnaround time can provide desirable alternatives for the rapid detection of Candida microbes. However, most of the published PCR primer sets are not Candida specific and likely to amplify DNA from common environmental contaminants, such as Aspergillus microbes. In this study, we designed pan-Candida primer sets based on the ribosomal DNA-coding regions conserved within Candida but distinct from those of Aspergillus and Penicillium. We demonstrate that the final two selected pan-Candida primer sets would not amplify Aspergillus DNA and could be used to differentiate eight medically important Candida pathogens in real-time PCR assays based on their melting profiles, with a sensitivity of detection as low as 10 fg of Candida genomic DNA. Moreover, we further evaluated and selected species-specific primer sets covering Candida albicans, Candida glabrata, Candida tropicalis, and Candida dubliniensis and show that they had high sensitivity and specificity. These real-time PCR primer sets could potentially be assembled into a single PCR array for the rapid detection of Candida species in various clinical settings, such as corneal transplantation. PMID:27103821

  20. Candida albicans escapes from mouse neutrophils

    DEFF Research Database (Denmark)

    Ermert, David; Niemiec, Maria J; Röhm, Marc;

    2013-01-01

    Candida albicans, the most commonly isolated human fungal pathogen, is able to grow as budding yeasts or filamentous forms, such as hyphae. The ability to switch morphology has been attributed a crucial role for the pathogenesis of C. albicans. To mimic disseminated candidiasis in humans, the mouse...... is the most widely used model organism. Neutrophils are essential immune cells to prevent opportunistic mycoses. To explore potential differences between the rodent infection model and the human host, we compared the interactions of C. albicans with neutrophil granulocytes from mice and humans. We...... revealed that murine neutrophils exhibited a significantly lower ability to kill C. albicans than their human counterparts. Strikingly, C. albicans yeast cells formed germ tubes upon internalization by murine neutrophils, eventually rupturing the neutrophil membrane and thereby, killing the phagocyte. On...

  1. Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans

    Directory of Open Access Journals (Sweden)

    Jesús M. Egido

    1997-12-01

    Full Text Available We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripherial blood (HCPB, using commercially available phycoerythrin-conjugated latex particles of 1µm diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984 standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripherial blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.Se da cuenta de un método rápido para la cuantización del flujo citométrico de la fagocitosis en sangre periférica completamente heparinizada (HCPB, mediante la utilización de partículas de látex phycoerythrin-conjugadas de 1µm de diámetro disponibles comercialmente. El método es más rápido y presenta mayor reproducibilidad que la técnica estandar de Bjerknes' (1984 utilizando propidium iodide-teñida Candida albicans, aplicada convencionalmente a la capa leucocitica de sangre periférica pero modificada por HCPB. Tambien damos cuenta de una modificación de Bjerknes' Intracellular Killing Test para permitir su aplicación a HCPB.

  2. Laser induced breakdown spectroscopy for the discrimination of Candida strains.

    Science.gov (United States)

    Manzoor, S; Ugena, L; Tornero-Lopéz, J; Martín, H; Molina, M; Camacho, J J; Cáceres, J O

    2016-08-01

    The present study reports the evaluation of Laser Induced Breakdown Spectroscopy (LIBS) and Neural Networks (NN) for the discrimination of different strains of various species of Candida. This genus of yeast was selected due to its medical relevance as it is commonly found in cases of fungal infection in humans. Twenty one strains belonging to seven species of Candida were included in the study. Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy (SEM-EDS) was employed as a complementary technique to provide information about elemental composition of Candida cells. The use of LIBS spectra in combination with optimized NN models provided reliable discrimination among the distinct Candida strains with a high spectral correlation index for the samples analyzed, without any false positive or false negative. Therefore, this study indicates that LIBS-NN based methodology has the potential to be used as fast fungal identification or even diagnostic method. PMID:27216662

  3. Recurrent Candida albicans Ventriculitis Treated with Intraventricular Liposomal Amphotericin B

    Directory of Open Access Journals (Sweden)

    Demet Toprak

    2015-01-01

    Full Text Available Central nervous system (CNS infection with Candida is rare but significant because of its high morbidity and mortality. When present, it is commonly seen among immunocompromised and hospitalized patients. Herein, we describe a case of a four-year-old boy with acute lymphoblastic leukemia (ALL who experienced recurrent Candida albicans meningitis. The patient was treated successfully with intravenous liposomal amphotericin B at first attack, but 25 days after discharge he was readmitted to hospital with symptoms of meningitis. Candida albicans was grown in CFS culture again and cranial magnetic resonance imaging (MRI showed ventriculitis. We administered liposomal amphotericin B both intravenously and intraventricularly and favorable result was achieved without any adverse effects. Intraventricular amphotericin B may be considered for the treatment of recurrent CNS Candida infections in addition to intravenous administration.

  4. Antifungal Activity of Local Anesthetics Against Candida Species

    Directory of Open Access Journals (Sweden)

    Acácio Gonçalves Rodrigues

    2000-01-01

    Full Text Available Objective: To evaluate the activity of benzydamine, lidocaine, and bupivacaine, three drugs with local anesthetic activity, against Candida albicans and non-albicans strains and to clarify their mechanism of activity.

  5. Candida dubliniensis in children´s dental plaque

    Czech Academy of Sciences Publication Activity Database

    Růžička, F.; Holá, V.; Kukletová, M.; Zacková, L.; Kuklová, J.; Horká, Marie

    Istanbul, 2008. s. 58. [International Congress of Mycology /12./. 05.08.2008-09.08.2008, Istanbul] R&D Projects: GA AV ČR IAAX00310701 Keywords : Candida dubliniensis * dental plaque * CIEF Subject RIV: CB - Analytical Chemistry, Separation

  6. Activation of isoenzyme Sapp 1p from Candida parapsilosis

    Czech Academy of Sciences Publication Activity Database

    Dostál, Jiří; Dlouhá, Helena; Maloň, Petr; Pichová, Iva; Hrušková-Heidingsfeldová, Olga

    Budapest : -, 2005. s. 15. [EMBO/HHMI. 07.02.2005-09.02.2005, Budapest] Institutional research plan: CEZ:AV0Z40550506 Keywords : Candida parapsilosis * aspartic proteases * processing and self-processing Subject RIV: CE - Biochemistry

  7. Antifungal activity of plant extracts against candida species from oral lesions

    Directory of Open Access Journals (Sweden)

    Prabhakar K

    2008-01-01

    Full Text Available Seventy five patients with oral lesions attending the different departments of Rajah Muthiah Medical College and Hospital, Annamalai University were screened for Candida . Forty six (61.3% Candida strains were isolated from the oral lesions. Of the 46 Candida strains, Candida albicans accounted for 35 (76.08%, Candida glabrata for 5 (10.86%, Candida tropicalis and Candida krusei for 2 (4.34% each and Candida parapsilosis and Candida guilliermondii for one (2.17% each. Antifungal activity of ethanol extracts of five plant species that included Syzygium jambolanum, Cassia siamea, Odina wodier, Momordica charantia and Melia azedarach and two algal species, Sargassum wightii and Caulerpa scalpelliformis were tested against 25 isolated strains by disc diffusion method. Antifungal activity was observed at 100 mg/ml for Syzygium jambolanum, Cassia siamea and Caulerpa scalpelliformis and at 10 mg/ml for Sargassum wightii .

  8. Analysis of gene evolution and metabolic pathways using the Candida Gene Order Browser

    LENUS (Irish Health Repository)

    Fitzpatrick, David A

    2010-05-10

    Abstract Background Candida species are the most common cause of opportunistic fungal infection worldwide. Recent sequencing efforts have provided a wealth of Candida genomic data. We have developed the Candida Gene Order Browser (CGOB), an online tool that aids comparative syntenic analyses of Candida species. CGOB incorporates all available Candida clade genome sequences including two Candida albicans isolates (SC5314 and WO-1) and 8 closely related species (Candida dubliniensis, Candida tropicalis, Candida parapsilosis, Lodderomyces elongisporus, Debaryomyces hansenii, Pichia stipitis, Candida guilliermondii and Candida lusitaniae). Saccharomyces cerevisiae is also included as a reference genome. Results CGOB assignments of homology were manually curated based on sequence similarity and synteny. In total CGOB includes 65617 genes arranged into 13625 homology columns. We have also generated improved Candida gene sets by merging\\/removing partial genes in each genome. Interrogation of CGOB revealed that the majority of tandemly duplicated genes are under strong purifying selection in all Candida species. We identified clusters of adjacent genes involved in the same metabolic pathways (such as catabolism of biotin, galactose and N-acetyl glucosamine) and we showed that some clusters are species or lineage-specific. We also identified one example of intron gain in C. albicans. Conclusions Our analysis provides an important resource that is now available for the Candida community. CGOB is available at http:\\/\\/cgob.ucd.ie.

  9. Using routine laboratory techniques for differentiation of pathogenic Candida strains

    Czech Academy of Sciences Publication Activity Database

    Vykydalová, Marie; Horká, Marie; Růžička, F.; Kahle, Vladislav

    Toruń: Adam Marszałek, 2012 - (Węgłowska, J.). s. 380 ISBN 978-83-7780-440-7. [International Symposium on Chromatography /29./. 09.09.2012-13.02.2012, Toruń] R&D Projects: GA MŠk(CZ) CZ.1.07/2.3.00/20.0182 Institutional support: RVO:68081715 Keywords : Candida parasilosis * Candida metapsilosis * biofilm * SDS-PAGE Subject RIV: CB - Analytical Chemistry, Separation

  10. Biofilm formation among Candida albicans isolated from vagina

    OpenAIRE

    2014-01-01

    Purpose: Study was conducted in a rural tertiary care hospital with a purpose to demonstrate the biofilm forming abilities of C. albicans isolated from cases of vulvovaginal candidiasis and asymptomatic carriers.Material and Methods: C. albicans was isolated and identified by standard laboratory techniques. Biofilm formation in vitro was tested using the 96 well microtitre plate method with crystal violet staining.Results: Overall rate of Candida isolation in study subjects was 40%. Candida i...

  11. Candida parapsilosis meningitis associated with Gliadel (BCNU) wafer implants.

    LENUS (Irish Health Repository)

    O'Brien, Deirdre

    2012-02-01

    A 58-year old male presented with meningitis associated with subgaleal and subdural collections 6 weeks following a temporal craniotomy for resection of recurrent glioblastoma multiforme and Gliadel wafer implantation. Candida parapsilosis was cultured from cerebrospinal fluid (CSF) and Gliadel wafers removed during surgical debridement. He was successfully treated with liposomal amphotericin B. To our knowledge, this is the first reported case of Candida parapsilosis meningitis secondary to Gliadel wafer placement.

  12. Candida parapsilosis meningitis associated with Gliadel (BCNU) wafer implants.

    LENUS (Irish Health Repository)

    O'brien, Deirdre

    2010-12-15

    A 58-year old male presented with meningitis associated with subgaleal and subdural collections 6 weeks following a temporal craniotomy for resection of recurrent glioblastoma multiforme and Gliadel wafer implantation. Candida parapsilosis was cultured from cerebrospinal fluid (CSF) and Gliadel wafers removed during surgical debridement. He was successfully treated with liposomal amphotericin B. To our knowledge, this is the first reported case of Candida parapsilosis meningitis secondary to Gliadel wafer placement.

  13. Successful management of presumed Candida endogenous endophthalmitis with oral voriconazole

    OpenAIRE

    Biju, Raju; Sushil, Daniel; Georgy, Nainan K

    2009-01-01

    Endogenous fungal endophthalmitis is most commonly caused by Candida species and usually occurs in patients with chronic diseases such as diabetes mellitus and renal insufficiency. Voriconazole, a broad-spectrum triazole antifungal agent, attains therapeutically significant concentrations in the vitreous cavity after systemic administration. We report, the successful management of presumed endogenous Candida endophthalmitis in a patient with multiple diseases and unstable systemic status with...

  14. Candida glabrata infection following total hip arthroplasty: A case report

    OpenAIRE

    Zhu, Yun; Yue, Chen; Huang, Zeyu; Pei, Fuxing

    2013-01-01

    Candida glabrata infection following total hip arthroplasty is rare and, due to the insufficiency of standardized clinical and evidence-based guidelines, there is no appropriate therapeutic schedule. The present study reports the case of a 44-year-old patient with Candida glabrata infection following a total hip arthroplasty. The patient was successfully treated by administration of intravenous and oral voriconazole without removal of the prosthesis. This case illustrates the significance of ...

  15. The Detection of Candida Species in Patients with Halitosis

    OpenAIRE

    2014-01-01

    To examine the effects of Candida on halitosis, the carrier state of Candida was examined in patients who made a visit with a chief complaint of halitosis. Methods. Subjects were 123 patients (42 males and 81 females) who visited our clinic, with a chief complaint of halitosis. Their average age was 45.8 years. To examine halitosis, an organoleptic test was conducted, and volatile sulfur compounds (VSCs) were measured by gas chromatography. Tongue-coating samples collected at the initial visi...

  16. Candida glabrata : a review of its features and resistance

    OpenAIRE

    Rodrigues, Célia F.; Silva, Sónia Carina; Henriques, Mariana

    2014-01-01

    Candida species belong to the normal microbiota of the oral cavity and gastrointestinal and vaginal tracts, and are responsible for several clinical manifestations, from mucocutaneous overgrowth to bloodstream infections. Once believed to be non-pathogenic, Candida glabrata was rapidly blamable for many human diseases. Year after year, these pathological circumstances are more recurrent and problematic to treat, especially when patients reveal any level of immunosuppression. These difficultie...

  17. Evolution of pathogenicity and sexual reproduction in eight Candida genomes

    OpenAIRE

    Butler, Geraldine; Rasmussen, Matthew D.; Lin, Michael F.; Santos, Manuel A. S.; Sakthikumar, Sharadha; Munro, Carol A; Rheinbay, Esther; Grabherr, Manfred; Forche, Anja; Reedy, Jennifer L.; Agrafioti, Ino; Arnaud, Martha B.; Bates, Steven; Brown, Alistair J. P.; Brunke, Sascha

    2009-01-01

    Candida species are the most common cause of opportunistic fungal infection worldwide. We report the genome sequences of six Candida species and compare these and related pathogens and nonpathogens. There are significant expansions of cell wall, secreted, and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diploid species, possibly resulting from recent recombination events. Surprisingly, key compo...

  18. Fermentation of fruit juices by the osmotolerant yeast Candida magnoliae

    OpenAIRE

    Medeiros, Andreia Sofia Soares de

    2014-01-01

    This study focuses on the assessment of the fermentation conditions required to modulate the metabolic flux in the osmotolerant yeast Candida magnoliae and evaluate its potential to produce low-alcoholic and low-caloric fermented beverages. For that purpose, two strains, PYCC 2903 and PYCC 3191, were used and fermentation conditions as oxygenation, sugar concentration and the ratio of glucose to fructose were studied using synthetic culture media. Candida magnoliae PYCC 2903 was subsequently ...

  19. Candida albicans Secreted Aspartyl Proteinases in Virulence and Pathogenesis

    OpenAIRE

    Naglik, Julian R.; Challacombe, Stephen J; Hube, Bernhard

    2003-01-01

    Candida albicans is the most common fungal pathogen of humans and has developed an extensive repertoire of putative virulence mechanisms that allows successful colonization and infection of the host under suitable predisposing conditions. Extracellular proteolytic activity plays a central role in Candida pathogenicity and is produced by a family of 10 secreted aspartyl proteinases (Sap proteins). Although the consequences of proteinase secretion during human infections is not precisely known,...

  20. Three distinct secreted aspartyl proteinases in Candida albicans.

    OpenAIRE

    White, T C; Miyasaki, S H; Agabian, N

    1993-01-01

    The secreted aspartyl proteinases of Candida albicans (products of the SAP genes) are thought to contribute to virulence through their effects on Candida adherence, invasion, and pathogenicity. From a single strain of C. albicans (WO-1) which expresses a phenotypic switching system, three secreted aspartyl proteinases have been identified as determined by molecular weight and N-terminal sequence. Each of the three identified proteins represents the mature form of one of three distinct protein...

  1. Interactions of Candida albicans with host epithelial surfaces

    OpenAIRE

    David W. Williams; Jordan, Rachael P. C.; Wei, Xiao-qing; Alves, Carlos T.; Wise, Matt P; Wilson, Melanie J.; Michael A. O. Lewis

    2013-01-01

    Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. The organism is however, commonly encountered as a commensal in healthy individuals where it is a component of the normal microflora. The key determinant in the type of relationship that Candida has with its host is how it interacts with the epithelial surface it colonises. A delicate balance clearly exis...

  2. Characterization of Candida isolates from pediatric burn patients.

    OpenAIRE

    Neely, A N; Odds, F.C.; Basatia, B K; Holder, I A

    1988-01-01

    To provide more detailed information about Candida epidemiology and pathogenesis in pediatric burn patients, Candida isolates from 113 patients collected over 3 years were identified at the species level and the serotypes and biotypes of the C. albicans isolates were determined. A total of 85% of the patients were colonized or infected by C. albicans, 18% by C. tropicalis, and 11% by C. parapsilosis. Although colonization or infection often was found at multiple sites and times, 87% of the pa...

  3. Aislamiento y caracterización de ABG1, un gen esencial del hongo patógeno oportunista Candida Albicans.

    OpenAIRE

    Veses Jiménez, Verónica

    2005-01-01

    RESUMEN Mediante el inmunorrastreo de una genoteca de expresión de Candida albicans con un anticuerpo policlonal específico de la forma micelial de dicho hongo (PAb anti-gt), se ha aislado un nuevo gen de este microorganismo. La represión del gen aislado ocasionó un severo defecto en la morfogénesis de C. albicans, caracterizado por la formación de cadenas de levaduras, de tamaño decreciente en dirección al extremo apical de la cadena, por lo que el gen fue denominado ABG1 (del inglés, alt...

  4. Miocarditis por dengue

    OpenAIRE

    Ricardo Amador García Hernández; Fidel Espinosa Rivera; Lianet Rivero Seriel

    2013-01-01

    El dengue es un arbovirus transmitido por el Aedes aegypti, produce los cuadros clínicos de dengue clásico, dengue hemorrágico y síndrome de choque por dengue, aisladamente se reportan casos de miocarditis. Se presentó un paciente con antecedentes de dengue clásico que manifestó dolor precordial asociado a cambios electrocardiográficos y fue ingresado en este hospital por síndrome coronario agudo probable, al cual se le diagnosticó clínicamente miocarditis por dengue.

  5. Mecanismos de modulación de la respuesta inmune por Chlamydia trachomatis asociados a infertilidad

    Directory of Open Access Journals (Sweden)

    Tatiana Marcela López-Castro

    2012-12-01

    Full Text Available Introducción: la infertilidad, problema clínico y social que afecta del 13 al 15% de las parejas en el mundo, es causada, entre otros, por la enfermedad pélvica inflamatoria ocasionada por varios agentes infecciosos entre los cuales se destaca la Chlamydia trachomatis. Este agente infeccioso posee mecanismos moleculares con los cuales modula la respuesta inmune del huésped y produce cambios en la célula infectada para permitir su supervivencia, ocasionando que la respuesta del sistema inmunológico se establezca en forma crónica, con la consecuente inflamación permanente y con ello secuelas como cicatrices y obstrucción de la trompa de Falopio. El objetivo de esta revisión es ofrecer una actualización de conocimiento en inmunobiología de la infección por Chlamydia trachomatis y su relación con la infertilidad. Materiales y métodos: se realizó una revisión de la literatura en diferentes bases de datos: PubMed/ Medline, Science Direct, Ovid, desde enero del año 1995 a enero del 2012, incluyendo artículos de revisión y estudios clínicos. Resultados: en la actualidad se sostiene que la inmunomodulación que caracteriza la infección por Chlamydia trachomatis, los mediadores inflamatorios implicados en la respuesta inmune, y la posible aunque poco estudiada susceptibilidad genética del huésped, se relacionan estrechamente con la génesis de la infertilidad por factor tubárico. Conclusión: la infertilidad causada por Chlamydia trachomatis tiene su origen en la respuesta inmunológica del huésped y en la modulación por parte de este agente infeccioso, lo que lleva a inflamación crónica, cicatrización y obstrucción de la trompa de Falopio.

  6. Mortalidad por envenenamiento en niños

    Directory of Open Access Journals (Sweden)

    Híjar Martha

    1998-01-01

    Full Text Available Objetivo. Conocer el panorama de las muertes por envenenamiento en niños de 0-14 años ocurridas en la República mexicana, entre 1979 y 1994. Material y métodos. Se utilizaron fuentes secundarias. Las variables analizadas fueron: edad, sexo, año, causa externa de traumatismos y envenenamientos, de la IX Clasificación Internacional de Enfermedades: E850-E858, E860-E869 y E905. Mediante un modelo de regresión Poisson se analizaron tendencias por causa específica y se obtuvieron riesgos relativos según edad, sexo y entidad federativa. Resultados. Hubo un total de 11 272 defunciones en menores de 15 años; las principales causas fueron el envenenamiento y las reacciones tóxicas causadas por plantas y animales venenosos (E905, el envenenamiento accidental por gas de uso doméstico y por monóxido de carbono (E868 y el envenenamiento accidental por otras drogas (E858. El grupo de edad que presentó los mayores riesgos, para las causas mencionadas, fue el de menores de un año con un riesgo relativo (RR de 29.6, IC95% 29.2-33.4; RR 3.47, IC95% 2.86-4.22, y RR 31.86, IC95% 24.8-40.9. El riesgo fue similar en ambos sexos, salvo para la causa E905. El estado de Aguascalientes se situó sistemáticamente entre los de mayor riesgo para todas las causas analizadas, mientras que Nuevo León siempre se ubicó entre los de riesgo más bajo. Conclusiones. El envenenamiento constituye una importante causa de muerte en los niños; el riesgo se incrementa al disminuir la edad. Considerando que esas muertes son potencialmente evitables y que la mayor parte de los envenenamientos ocurren en el hogar, para prevenirlos, se recomienda a los familiares vigilar y mantener fuera de peligro al niño. Por otra parte, la multicausalidad del fenómeno requiere que su prevención se realice desde una perspectiva multidisciplinaria que genere una cultura y un ambiente de seguridad en la sociedad.

  7. The importance of genus Candida in human samples

    Directory of Open Access Journals (Sweden)

    Bojić-Miličević Gordana M.

    2008-01-01

    Full Text Available Microbiology is a rapidly changing field. As new researches and experiences broaden our knowledge, changes in the approach to diagnosis and therapy have become necessary and appropriate. Recommended dosage of drugs, method and duration of administration, as well as contraindications to use, evolve over time all drugs. Over the last 2 decades, Candida species have emerged as causes of substantial morbidity and mortality in hospitalized individuals. Isolation of Candida from blood or other sterile sites, excluding the urinary tract, defines invasive candidiasis. Candida species are currently the fourth most common cause of bloodstream infections (that is, candidemia in U.S. hospitals and occur primarily in the intensive care unit (ICU, where candidemia is recognized in up to 1% of patients and where deep-seated Candida infections are recognized in an additional 1 to 2% of patients. Despite the introduction of newer anti-Candida agents, invasive candidiasis continues to have an attributable mortality rate of 40 to 49%; excess ICU and hospital stays of 12.7 days and 15.5 days, respectively, and increased care costs. Postmortem studies suggest that death rates related to invasive candidiasis might, in fact, be higher than those described because of undiagnosed and therefore untreated infection. The diagnosis of invasive candidiasis remains challenging for both clinicians and microbiologists. Reasons for missed diagnoses include nonspecific risk factors and clinical manifestations, low sensitivity of microbiological culture techniques, and unavailability of deep tissue cultures because of risks associated with the invasive procedures used to obtain them. Thus, a substantial proportion of invasive candidiasis in patients in the ICU is assumed to be undiagnosed and untreated. Yet even when invasive candidiasis is diagnosed, culture diagnosis delays treatment for 2 to 3 days, which contributes to mortality. Interventions that do not rely on a specific

  8. Influence of probiotics on Candida presence and IgA anti-Candida in the oral cavity

    OpenAIRE

    dos Santos, Agda Lima; Antônio Olavo Cardoso JORGE; dos Santos, Silvana Soléo Ferreira; Célia Regina Gonçalves e Silva; Leão, Mariella Vieira Pereira

    2009-01-01

    Probiotics are defined as microorganisms that promote benefits to host health, mainly by regulating resident microbiota. Disequilibrium in microbiota can favor the growth of opportunist microorganisms and the development of pathologies, like candidosis caused by yeasts of the Candida genus. This work evaluated whether probiotics consumption was able to influence a specific immunological response to Candida and the presence of these yeasts in the oral cavity. Saliva samples were collected from...

  9. Intoxicación por ciguatera: Estudio de 227 pacientes durante el periodo 1999 al 2005 Ciguatera poisoning: Study of 277 patients in the 1999-2005 period.

    OpenAIRE

    Carlos Manuel Maya Entenza; Miladys Martín Labrador; Marisol Monteagudo Torres

    2007-01-01

    La ciguatera es la intoxicación alimentaría causada por el consumo de pescados ciguatóxicos, cuyos efectos sobre el ser humano pueden ser graves y conducir a la muerte. Se describieron las principales características epidemiológicas, clínicas y del tratamiento en los pacientes con ciguatera. La investigación incluyó 227 pacientes con ciguatera estudiados de forma prospectiva en el Hospital Clínico-Quirúrgico Universitario “Comandante Manuel Fajardo” de Ciudad de La Habana entre lo...

  10. Pancreatite aguda devida a hematoma intramural do duodeno por uso de anticoagulante Acute pancreatitis due to intramural hematoma of the duodenum by use of anticoagulant therapy

    OpenAIRE

    Samer FARHOUD; Simone Moraes STEPHANI; Sansom Henrique BROMBERG

    2001-01-01

    Racional - A hemorragia intramural espontânea do duodeno causada por complicações da terapêutica anticoagulante é rara e seu tratamento controverso. Objetivo -- Apresentar a experiência advinda do tratamento de doente com essa condição clínica. Relato do caso - Expõe-se o caso de uma mulher de 71 anos de idade, que há 3 meses fazia uso de anticoagulante oral para tratamento de trombose venosa profunda dos membros inferiores. Apresentou-se com cefaléia e dores abdominais intensas no andar supe...

  11. Natural extracts from wild flowers used in Portuguese folk medicine like a new antifungal agents against Candida species

    OpenAIRE

    Alves, Carlos; Ferreira, Isabel C. F. R.; Barros, Lillian; Silva, Sónia Carina; Oliveira, Rosário; Henriques, Mariana

    2012-01-01

    The prevalence of opportunistic fungal infections has been increasing dramatically over the recent decades mainly due to the boom of the AIDS epidemic, increasing number of immunocompromised patients and the commonly use of indwelling medical devices. Although Candida albicans has been regarded as the most common causative agent of fungal infection in humans, nowadays other non-Candida albicans Candida (NCAC) species such as Candida glabrata, Candida tropicalis and Candida parapsilosis, are e...

  12. Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso Mycological aspects and susceptibility in vitro the yeast of the genus Candida from HIV-positive patients in the State of Mato Grosso

    Directory of Open Access Journals (Sweden)

    Olivia Cometti Favalessa

    2010-12-01

    Full Text Available INTRODUÇÃO: A candidíase é uma das infecções fúngicas mais frequentes entre os pacientes infectados pelo vírus da imunodeficiência humana. O presente estudo objetivou a caracterização das leveduras do gênero Candida de distintas amostras clínicas, provenientes de pacientes HIV - positivos, assim como a determinação do perfil de suscetibilidade in vitro a cinco drogas antifúngicas. MÉTODOS: A caracterização dos isolados de Candida sp foi realizada através da metodologia clássica, testes bioquímicos (zimograma e auxanograma e morfológicos (prova do tubo germinativo e microcultivo em lâmina. Também, foram realizadas a técnica genotípica (PCR e identificação pelo método comercial API 20C AUX (BioMeriéux. Para a determinação do perfil de suscetibilidade in vitro, foram utilizadas cinco drogas antifúngicas (cetoconazol, fluconazol, itraconazol, voriconazol e anfotericina B, através do método comercialmente disponível - Etest. RESULTADOS: Foram identificados 105 isolados de leveduras do gênero Candida provenientes de 102 pacientes infectados pelo vírus HIV. Destes, foram caracterizadas 82 (78,1% Candida albicans, 8 (7,6% Candida parapsilosis, 8 (7,6% Candida tropicalis, 4 (3,8% Candida krusei, 2 (1,9% Candida glabrata e 1 (1% Candida guilliermondii. CONCLUSÕES: Considerando o perfil geral de sensibilidade, 60% dos isolados foram suscetíveis a todos os antifúngicos testados, porém as espécies C. tropicalis e C. krusei demonstraram uma tendência a valores mais elevados de CIMs para os azóis do que os encontrados paraC. albicans, sugerindo resistência.INTRODUCTION: Candidiasis is one of the most common fungal infections among patients infected by human immunodeficiency virus. The present study aimed to characterize yeasts of the genus Candida from distinct clinical samples from HIV-positive patients and determine the in vitro susceptibility profile to five antifungal drugs. METHODS: Characterization of

  13. Estudo de hemoparasitas transmitidos por vectores, em cães de canil, Setúbal, Portugal

    OpenAIRE

    Silva, Inês Picanço Castanheira da

    2011-01-01

    As doenças transmitidas por vectores são causadas por parasitas, bactérias ou vírus e são veiculadas pela picada de artrópodes (principalmente ixodídeos e mosquitos). Estas doenças afectam cães a nível mundial e a maioria delas têm potencial zoonótico. Nestas estão incluídas as doenças sob estudo: Erliquiose, Babesiose, Riquetsiose, Anaplasmose e Leishmaniose. A patogénese das doenças sob estudo, baseia-se fundamentalmente na resposta humoral exagerada e não protectora desenvolvida pelo ho...

  14. Medical treatment of a pacemaker endocarditis due to Candida albicans and to Candida glabrata.

    Science.gov (United States)

    Roger, P M; Boissy, C; Gari-Toussaint, M; Foucher, R; Mondain, V; Vandenbos, F; le Fichoux, Y; Michiels, J F; Dellamonica, P

    2000-09-01

    We describe a case of pacemaker infection due to two fungal species: Candida albicans and C. glabrata. Transthoracic echocardiography showed a large vegetation on the intraventricular wires. Because of severe underlying diseases, surgery was believed to be contraindicated. The patient was treated using high dose of fluconazole, resulting in clinical improvement and negative blood cultures. However, 2 months later, the patient underwent a fatal stroke. At autopsy, a large vegetation was found only all along the wires. Postmortem culture of the infected material was positive for both C. albicans and C. glabrata. PMID:11023765

  15. SUSCEPTIBILITY OF CANDIDA SPECIES TO ANTIFUNGAL DRUGS IN WESTERN INDIA

    Directory of Open Access Journals (Sweden)

    Geeta M Vaghela

    2015-06-01

    Full Text Available Introduction: The increase in candidaemia is associated with high mortality. A shift has been observed in the relative frequency of each Candida spp. isolated from blood. Options of the antifungal drugs available for treatment of systemic and invasive candidiasis are restricted to polyenes, allylamines, azoles and recently developed echinocandin class of molecules. A rise in the incidence of antifungal resistance to Candida spp. has also been reported over the past decade. Studies on prevalence of infections and antifungal susceptibility testing are useful in deciding clinical strategies. Aims: To do species level identification and detect resistance, if any, among Indian clinical isolates of C. albicans. Methodology: From total 135 patients from a tertiary care hospital of Gujarat, Candida species were isolated from different clinical specimens. The growth of Candida on Sabouraud's dextrose agar was confirmed by Gram staining in which gram positive budding fungal cells were observed. Then its growth was examined for colony morphology on Sabouraud's dextrose agar and chlamydospore production on Corn meal tween 80 agar. Germ tube tests and other biochemical tests like sugar fermentation, sugar assimilation and urease test were performed to identify the species of Candida. Antifungal susceptibility testing was performed by NCCLS M44-A Disc diffusion method. Results: Out of total 135 samples, C. Albicans were isolated from 52 (38.5%. Among Non Albican Candid (NAC, Candida glabrata was 36 (26.7% followed by Candida tropicalis 25(18.5%. C. albicans was found resistant to Fluconazole, Itraconazole and Amphotericine B in 3.8%, 3.8% and 1.9% cases respectively. For NAC, resistance of Fluconazole, Itraconazole and Amphotericine B was found in 4.8%, 3.6% and 2.4% cases respectively. [Natl J Med Res 2015; 5(2.000: 122-126

  16. Proinflammatory chemokines during Candida albicans keratitis.

    Science.gov (United States)

    Yuan, Xiaoyong; Hua, Xia; Wilhelmus, Kirk R

    2010-03-01

    Chemotactic cytokines mediate the recruitment of leukocytes into infected tissues. This study investigated the profile of chemokines during experimental Candida albicans keratitis and determined the effects of chemokine inhibition on leukocyte infiltration and fungal growth during murine keratomycosis. Scarified corneas of BALB/c mice were topically inoculated with C. albicans and monitored daily over one week for fungal keratitis. After a gene microarray for murine chemokines compared infected corneas to controls, real-time reverse transcription polymerase chain reaction (RT-PCR) and immunostaining assessed chemokine expression in infected and mock-inoculated corneas. An anti-chemokine antibody was then administered subconjunctivally and evaluated for effects on clinical severity, corneal inflammation, fungal recovery, and cytokine expression. Of 33 chemokine genes examined by microarray, 6 CC chemokines and 6 CXC chemokines were significantly (Pamount of recoverable fungi was not significantly (P=0.4) affected. Anti-CCL3 treatment significantly (P=0.01) reduced the expression of tumor necrosis factor and interleukin-1beta in infected corneas. These results indicate that chemokines, especially the CC chemokine CCL3, play important roles in the acute inflammatory response to C. albicans corneal infection. PMID:20005222

  17. Studies of Immune Responses in Candida vaginitis.

    Science.gov (United States)

    De Bernardis, Flavia; Arancia, Silvia; Sandini, Silvia; Graziani, Sofia; Norelli, Sandro

    2015-01-01

    The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs) and antibody (Abs)-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7) was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2) (PEV7), has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis. PMID:26473934

  18. Studies of Immune Responses in Candida vaginitis

    Directory of Open Access Journals (Sweden)

    Flavia De Bernardis

    2015-10-01

    Full Text Available The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs and antibody (Abs-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7 was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2 (PEV7, has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis.

  19. Triclosan antagonizes fluconazole activity against Candida albicans.

    LENUS (Irish Health Repository)

    Higgins, J

    2012-01-01

    Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg\\/L. However, at subinhibitory concentrations (0.5-2 mg\\/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.

  20. Farnesol-induced apoptosis in Candida albicans.

    Science.gov (United States)

    Shirtliff, Mark E; Krom, Bastiaan P; Meijering, Roelien A M; Peters, Brian M; Zhu, Jingsong; Scheper, Mark A; Harris, Megan L; Jabra-Rizk, Mary Ann

    2009-06-01

    Farnesol, a precursor in the isoprenoid/sterol pathway, was recently identified as a quorum-sensing molecule produced by the fungal pathogen Candida albicans. Farnesol is involved in the inhibition of germination and biofilm formation by C. albicans and can be cytotoxic at certain concentrations. In addition, we have shown that farnesol can trigger apoptosis in mammalian cells via the classical apoptotic pathways. In order to elucidate the mechanism behind farnesol cytotoxicity in C. albicans, the response to farnesol was investigated, using proteomic analysis. Global protein expression profiles demonstrated significant changes in protein expression resulting from farnesol exposure. Among the downregulated proteins were those involved in metabolism, glycolysis, protein synthesis, and mitochondrial electron transport and the respiratory chain, whereas proteins involved in folding, protection against environmental and oxidative stress, actin cytoskeleton reorganization, and apoptosis were upregulated. Cellular changes that accompany apoptosis (regulated cell death) were further analyzed using fluorescent microscopy and gene expression analysis. The results indicated reactive oxygen species accumulation, mitochondrial degradation, and positive terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) in the farnesol-exposed cells concurrent with increased expression of antioxidant-encoding and drug response genes. More importantly, the results demonstrated farnesol-induced upregulation of the caspase gene MCA1 and the intracellular presence of activated caspases. In conclusion, this study demonstrated that farnesol promotes apoptosis in C. albicans through caspase activation, implying an important physiological role for farnesol in the fungal cell life cycle with important implications for adaptation and survival. PMID:19364863

  1. Studies of Immune Responses in Candida vaginitis

    Science.gov (United States)

    De Bernardis, Flavia; Arancia, Silvia; Sandini, Silvia; Graziani, Sofia; Norelli, Sandro

    2015-01-01

    The widespread occurrence of vaginal candidiasis and the development of resistance against anti-fungal agents has stimulated interest in understanding the pathogenesis of this disease. The aim of our work was to characterize, in an animal model of vaginal candidiasis, the mechanisms that play a role in the induction of mucosal immunity against C. albicans and the interaction between innate and adaptive immunity. Our studies evidenced the elicitation of cell-mediated immunity (CMIs) and antibody (Abs)-mediated immunity with a Th1 protective immunity. An immune response of this magnitude in the vagina was very encouraging to identify the proper targets for new strategies for vaccination or immunotherapy of vaginal candidiasis. Overall, our data provide clear evidence that it is possible to prevent C. albicans vaginal infection by active intravaginal immunization with aspartyl proteinase expressed as recombinant protein. This opens the way to a modality for anti-Candida protection at the mucosa. The recombinant protein Sap2 was assembled with virosomes, and a vaccine PEVION7 (PEV7) was obtained. The results have given evidence that the vaccine, constituted of virosomes and Secretory aspartyl proteinase 2 (Sap2) (PEV7), has an encouraging therapeutic potential for the treatment of recurrent vulvovaginal candidiasis. PMID:26473934

  2. Diarrea crónica refractaria y malabsorción secundaria a hipogammaglobulinemia común variable, infestación crónica por giardia lamblia y gastrectomía total por adenocarcinoma gástrico: un manejo nutricional complejo Chonic diarrhea and malabsorption due to common variable immunodeficiency, gastrectomy and giardiasis infection: a difficult nutritional management

    OpenAIRE

    M. E. Domínguez-López; I. González-Molero; C. P. Ramírez-Plaza; Soriguer, F. (Federico); G. Olveira

    2011-01-01

    El adenocarcinoma gástrico es una de las causas más frecuentes de mortalidad en el mundo, siendo la cirugía el único tratamiento potencialmente curativo, aunque los efectos adversos digestivos y nutricionales son frecuentes y abundantes. La hipogammaglobulinemia variable común es causa de frecuentes manifestaciones digestivas, derivándose las más importantes en diarrea crónica causada por giardiasis, hiperplasia nodular linfoide o atrofia vellosa, siendo frecuente la malabsorción y la desnutr...

  3. Coaggregation of oral Candida isolates with bacteria from bone marrow transplant recipients.

    OpenAIRE

    Hsu, L Y; Minah, G E; Peterson, D. E.; Wingard, J R; Merz, W G; Altomonte, V; Tylenda, C A

    1990-01-01

    In vitro coaggregation between Candida species isolated from immunosuppressed bone marrow transplant recipients and oral bacteria was investigated. Each Candida strain showed a different pattern of coaggregation with the 22 bacterial strains studied. Two strains of Lactobacillus amylovorus isolated from separate bone marrow transplant patients and Fusobacterium nucleatum (VPI 10197) coaggregated with all Candida strains. Ten bacterial strains showed no coaggregation with the Candida strains. ...

  4. Influence of saliva and mucin on the adhesion of Candida oral clinical isolates

    OpenAIRE

    Seabra, C.; Botelho, C.M.; Oliveira, Ana C. N.; Henriques, Mariana

    2015-01-01

    Objectives: This research work intends to clarify the role of artificial saliva, in particularly the role of mucin, a salivary protein, on the surface properties and adhesion ability of Candida spp. oral clinical isolates to abiotic surfaces. Methods: Four oral clinical isolates of Candida spp. were used: two Candida albicans strains (AC; AM) and two Candida parapsilosis strains (AD; AM2). The strains were isolated from patients using oral prosthesis. The microorganisms were cultured in the a...

  5. Effect of Amylase, Papaein and Pepsin enzyme solutions on Candida biofilm formed on acrylic resin plates

    OpenAIRE

    A Jafari Nodoushan; A Fallah Tafti; Emami, P; H Ashoori

    2013-01-01

    Abstract:     Background and Aim: Denture stomatitis results from colonization of oral Candida on the surface of denture acrylic base. To control this infection,Candida biofilm formation must be prevented using mechanical and chemical decontamination. The purpose of the present study was to evaluate the effect of Amylase, Papaein and Pepsin solutions on removal of Candida Albicans plaques formed on acrylic resin plates.   Materials and Methods : In this experimental study Candida biofilm was ...

  6. Effect of Amylase, Papaein and Pepsin enzyme solutions on Candida biofilm formed on acrylic resin plates

    OpenAIRE

    AA jafari_nodoushan; FalahTafti A; Emmami P; Ashouri

    2013-01-01

    Background and Aim: Denture stomatitis results from colonization of oral Candida on the surface of denture acrylic base. To control this infection,Candida biofilm formation must be prevented using mechanical and chemical decontamination. The purpose of the present study was to evaluate the effect of Amylase, Papaein and Pepsin solutions on removal of Candida Albicans plaques formed on acrylic resin plates. Materials and Methods: In this experimental study Candida biofilm was formed on 220 ...

  7. Eucalyptus globulus L. extract as an antifungal agent against Candida species

    OpenAIRE

    Martins, Natália; Ferreira, Isabel C. F. R.; Barros, Lillian; Henriques, Mariana; Silva, Sónia

    2014-01-01

    Hundreds of botanical preparations have been prepared and used for a wide variety of conditions. Different studies have been carried out towards to confirm the effective multitude of health benefits provided by medicinal plants [I]. In parallel with these studies, in the last years, high rates of opportunistic fungal infections have been observed, namely involving Candida species. Candida albicans is the most common but, currently, other non-Candida albicans Candida (NCAC) species have also b...

  8. In vitro evaluation of proteinase, phospholipase and haemolysin activities of Candida species isolated from clinical specimens

    OpenAIRE

    Sachin C.D; Ruchi K; Santosh S.

    2012-01-01

    Background: Virulence attributes of Candida species include adherence to host tissues, morphological changes and secretion of extracellular hydrolytic enzymes. These enzymes play pivotal roles in pathogenicity of candida infection. Aim: The present study aimed to determine phospholipase, proteinase and haemolysin activities in Candida species isolated from various clinical samples. Material and Method: A total of 110 Candida species isolated from various clinical specimens were identified up ...

  9. Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans.

    LENUS (Irish Health Repository)

    Jackson, Andrew P

    2009-12-01

    Candida dubliniensis is the closest known relative of Candida albicans, the most pathogenic yeast species in humans. However, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, C. dubliniensis is a significantly less virulent and less versatile pathogen. Therefore, to identify C. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the C. dubliniensis genome and compared it with the known C. albicans genome sequence. Although the two genome sequences are highly similar and synteny is conserved throughout, 168 species-specific genes are identified, including some encoding known hyphal-specific virulence factors, such as the aspartyl proteinases Sap4 and Sap5 and the proposed invasin Als3. Among the 115 pseudogenes confirmed in C. dubliniensis are orthologs of several filamentous growth regulator (FGR) genes that also have suspected roles in pathogenesis. However, the principal differences in genomic repertoire concern expansion of the TLO gene family of putative transcription factors and the IFA family of putative transmembrane proteins in C. albicans, which represent novel candidate virulence-associated factors. The results suggest that the recent evolutionary histories of C. albicans and C. dubliniensis are quite different. While gene families instrumental in pathogenesis have been elaborated in C. albicans, C. dubliniensis has lost genomic capacity and key pathogenic functions. This could explain why C. albicans is a more potent pathogen in humans than C. dubliniensis.

  10. Evaluation of Urinary Tract Infections Due to Candida Species

    Directory of Open Access Journals (Sweden)

    Yeser Karaca Derici

    2016-02-01

    Full Text Available Aim: Although urinary tract infections often caused by bacteria, fungal etiology is detected in a significant number of infections in which Candida is the leading cause. In this study we aimed to evaluate the distribution of Candida strains isolated from urine samples in our hospital. Material and Method: Candida species were identified based on germ tube test, colony morphology on chrom agar Candida (Biomerieux, France and API ID32C AUX (Biomerieux, France commercial kit. Data were analyzed with SPSS 15.0 software for data analysis. Results: During March 2011-March 2014 a total of 109662 urine cultures were evaluated and 24364 samples revealed significant growth. Of the significant growth detected 24364 (22% samples 1096 (4.5% were defined as yeasts. The isolates most frequently detected in this study were C. albicans (50.5%, C. tropicalis (15.9%, C. glabrata (12.7%, C. parapsilosis (7.2%, C. kefyr (5.8%, C. krusei (5.5%. The highest yeast growth was observed in anesthesia intensive care unit. Discussion: In our study, the most frequently isolated species of yeast in the urine was C. albicans. Determination of Candida species and their clinical distributions in hospitals is very important in terms of giving direction to the treatment and measures to be taken.

  11. Candida-Bacteria Interactions: Their Impact on Human Disease.

    Science.gov (United States)

    Allison, Devon L; Willems, Hubertine M E; Jayatilake, J A M S; Bruno, Vincent M; Peters, Brian M; Shirtliff, Mark E

    2016-06-01

    Candida species are the most common infectious fungal species in humans; out of the approximately 150 known species, Candida albicans is the leading pathogenic species, largely affecting immunocompromised individuals. Apart from its role as the primary etiology for various types of candidiasis, C. albicans is known to contribute to polymicrobial infections. Polymicrobial interactions, particularly between C. albicans and bacterial species, have gained recent interest in which polymicrobial biofilm virulence mechanisms have been studied including adhesion, invasion, quorum sensing, and development of antimicrobial resistance. These trans-kingdom interactions, either synergistic or antagonistic, may help modulate the virulence and pathogenicity of both Candida and bacteria while uniquely impacting the pathogen-host immune response. As antibiotic and antifungal resistance increases, there is a great need to explore the intermicrobial cross-talk with a focus on the treatment of Candida-associated polymicrobial infections. This article explores the current literature on the interactions between Candida and clinically important bacteria and evaluates these interactions in the context of pathogenesis, diagnosis, and disease management. PMID:27337476

  12. Successful treatment of Candida parapsilosis (fluconazole-resistant) osteomyelitis with caspofungin in a HIV patient.

    Science.gov (United States)

    Legout, L; Assal, M; Rohner, P; Lew, D; Bernard, L; Hoffmeyer, P

    2006-01-01

    Treating Candida arthritis is challenging. We report a case of Candida parapsilosis arthritis successfully treated with caspofungin. We illustrate the likelihood of severe infections due fluconazole resistant C. parapsilosis after extensive fluconazole use and discuss the role of newer antifungal agents in the treatment of arthritis due to Candida spp. PMID:16857628

  13. The influence of chemical composition of commercial lemon essential oils on the growth of Candida strains.

    Science.gov (United States)

    Białoń, M; Krzyśko-Łupicka, T; Koszałkowska, M; Wieczorek, P P

    2014-02-01

    Candida yeasts are saprophytes naturally present in the environment and forming colonies on human mucous membranes and skin. They are opportunistic fungi that cause severe and even fatal infections in immunocompromised individuals. Several essential oils, including eucalyptus, pine, cinnamon and lemon, have been shown to be effective against Candida strains. This study addresses the chemical composition of some commercial lemon essential oils and their antifungal potential against selected Candida yeast strains. Antifungal potential and minimum inhibitory concentrations were determined for six commercial lemon essential oils against five Candida yeast strains (Candida albicans 31, Candida tropicalis 32, Candida glabrata 33, Candida glabrata 35 and Candida glabrata 38). On the basis of the GCMS analysis, it was found that the tested lemon essential oils had different chemical compositions, but mostly, they contained almost exclusively terpenes and oxygenated terpenes. The tests show that antifungal potential of lemon essential oils against Candida yeast strains was related to the high content of monoterpenoids and the type of Candida strains. From six tested commercial oils, only four (ETJA, Vera-Nord, Avicenna-Oil and Aromatic Art) shows antifungal potential against three Candida species (C. albicans, C. tropicalis and C. glabrata). Vera-Nord and Avicenna-Oil show the best activity and effectively inhibit the growth of the C. albicans strain across the full range of the concentrations used. Our study characterises lemon essential oils, which could be used as very effective natural remedies against candidiasis caused by C. albicans. PMID:24436010

  14. Candida in saliva of Brazilian hemophilic patients Candida na saliva de pacientes hemofílicos brasileiros

    Directory of Open Access Journals (Sweden)

    Claudio Maranhão Pereira

    2004-12-01

    Full Text Available Hemophilia is a common hereditary hemorrhagic disorder, however little is known about the oral microflora of hemophilic patients. The aim of this study was to quantify the Candida and identify its species in non-stimulated saliva of hemophilic patients, and consider its relationship with clinical factors influencing Candida carriage. This study comprised evaluation of 86 hemophilic patients of the Hematology Center/UNICAMP and 43 healthy subjects as controls. All patients were submitted to anamnesis, intraoral examination and unstimulated saliva collection. Candida counts and species identification were performed in salivary samples. Candida was present in 64% of the hemophilic patients and in 44% of the healthy controls. C. albicans represented 65% and 68% of the isolated species, in hemophiliacs and control group respectively, and C. tropicalis was the second most common species in both groups. These results indicate that hemophilic patients carry Candida more frequently and in higher counts than healthy controls, independently of oral clinical parameter considered, as viral infections, complete dentures, transfusions of hemoderivatives, and salivary flow.Hemofilia é uma alteração hemorrágica hereditária comum, entretanto pouco se sabe a respeito da microbiota oral destes indivíduos. O objetivo deste estudo foi quantificar a presença de Candida e identificar as suas espécies na saliva de hemofílicos, correlacionando os resultados com fatores clínicos que possam influenciar a presença deste fungo. Foram avaliados 86 hemofílicos do Hemocentro/UNICAMP e 43 indivíduos saudáveis. Todos os pacientes foram submetidos a anamnese, exame clínico intra-oral e coleta de saliva de forma não estimulada. A quantificação e identificação das espécies de Candida foram realizadas nas amostras de saliva. Candida estava presente em 64% dos hemofílicos e em 44% dos indivíduos saudáveis. C. albicans representou 65% e 68% das esp

  15. Mechanisms of antifungal drug resistance in Candida dubliniensis.

    LENUS (Irish Health Repository)

    Coleman, David C

    2010-06-01

    Candida dubliniensis was first described in 1995 and is the most closely related species to the predominant human fungal pathogen Candida albicans. C. dubliniensis is significantly less prevalent and less pathogenic than C. albicans and is primarily associated with infections in HIV-infected individuals and other immunocompromised cohorts. The population structure of C. dubliniensis consists of three well-defined major clades and is significantly less diverse than C. albicans. The majority of C. dubliniensis isolates are susceptible to antifungal drugs commonly used to treat Candida infections. To date only two major patterns of antifungal drug resistance have been identified and the molecular mechanisms of these are very similar to the resistance mechanisms that have been described previously in C. albicans. However, significant differences are evident in the predominant antifungal drug mechanisms employed by C. dubliniensis, differences that reflect its more clonal nature, its lower prevalence and characteristics of its genome, the complete sequence of which has only recently been determined.

  16. Estudo terapêutico aberto com sulfato de aminosidine na leishmaniose mucosa causada for Leishmania (viannia braziliensis

    Directory of Open Access Journals (Sweden)

    Gustavo A.S. Romero

    1996-12-01

    Full Text Available No período de setembro a novembro de 1994 foram tratados 21 pacientes com leishmaniose mucosa ativa, predominantemente adultos lavradores do sexo masculino, com sulfato de aminosidine intramuscular, I6mg/kg/dia por 20 dias. Treze pacientes eram virgens de tratamento e 8 haviam sido tratados sem sucesso com Glucantime®". O diagnóstico baseou-se inicialmente em crítêrios epidemiolôgicos, clínicos e nos resultados da intrademoireação de Montenegro e a imunofluorescência indireta para anticoipos séricos antileishmânia e durante o acompanhamento nos resultados dos estudos parasitológicos. Sessenta e sete por cento dos pacientes tiveram diagnóstico parasitológico confirmado sendo a inoculação do material de biópsia das lesões em hamsters o método mais sensível. O tempo médio de acompanhamento foi de 12,6 meses. A adesão ao tratamento foi de 100%. Os efeitos colaterais foram dor no local da injeção (86%, proteinúria leve (24%, elevação do nível sérico de creatinina (5% e perda auditiva subclínica em um dos dois pacientes que realizaram audiometria. Obsevou-se cura clínica em 48% dos pacientes e a percentagem acumulada de recidiva foi de 29% (4/14pacientes durante o acompanhamento.

  17. FLUCONAZOLE SUSCEPTIBILITY TESTING OF CANDIDA SPECI ES BY DISC DIFFUSION AND AGAR DILUTION METHOD

    Directory of Open Access Journals (Sweden)

    Supriya

    2012-10-01

    Full Text Available PURPOSE: Fungal infections with candida species are an import ant cause of morbidity and mortality 1 . Situation is further worsened by increasing resis tance to antifungal drugs 2 .The objective of this study was to evaluate antifu ngal susceptibility pattern of Candida species to fluconazole by disc diffusion and agar di lution method and compare these two methods as far as fluconazole is concerned. MATERIAL & METHODS: Between January 2006 to December 2006, 119 Candida species were isolated fro m 225 sputum samples of patients of pulmonary tuberculosis and immunocomprised patients. RESULTS: Of the 119 Candida isolates 33 (27.73% were Candida albicans, 32 (26.89% were Candida gullermondi, 27 (22.68% Candida tropicalis, 14 (11.76% Candida pseudotropica lis, 7 (5.88% Candida parapsilosis and 6 (5.04% were Candida krusei. The Candida isolates w ere tested for fluconazole by disc diffusion method on Muller hinton agar with 2% glucose and 0. 5 microgram of methylene blue 3 .Amongst the 119 Candida isolates, 17 (14.28% isolates were resistant to fluconazole by disc diffusion method. MIC done by agar dilution method using CLSI guidelines 4. Candida isolates showed growth of 15 (88 .23% Candida strains upto 8 micro gram / ml with 80% inhibition of growth whereas 2 (1.7% isolates showed MIC upto 64 microgr am/ ml. CONCLUSIONS: Candida albicans though a common species other species were i solated in significant number. The results of disc diffusion for fluconazole do not co rrelate with agar dilution method. To cater the need of resource constrained laboratories, it is im portant to have results that correspond to the clinical outcome of antifungal treatment and show c o-relation to those obtained with the reference method as recommended by CLSI guidelines and also be reproducible 5 . Further critical studies are needed

  18. Native valve candida endocarditis complicating pregnancy after abdominal surgery

    Directory of Open Access Journals (Sweden)

    P Saphina

    2015-01-01

    Full Text Available Candida endocarditis is an emerging infectious disease, usually involving patients with intravascular prosthetic devices, and associated with substantial morbidity and mortality. A 28-year-old primigravida at 32 weeks of gestation was admitted with low-grade fever and lower abdominal pain for 2 weeks. She had undergone open appendicectomy 2 months before admission. Echocardiogram showed a pedunculated 24 mm × 21 mm mass attached to the undersurface of anterior mitral leaflet near the tip and moderate mitral regurgitation. Repeated blood cultures showed growth of nonalbicans candida. She was immediately started on liposomal amphotericin and was taken up for surgery, but despite all efforts she succumbed to her illness.

  19. Invasive Candida Infections in the ICU: Diagnosis and Therapy

    Directory of Open Access Journals (Sweden)

    Hankovszky Péter

    2015-10-01

    Full Text Available Invasive fungal infections have become a serious problem in the critically ill. One of the main reasons is the development of an immunocompromised condition. The most frequently found pathogens are Candida species. In order to provide adequate treatment, understanding this potentially life-threatening infection is mandatory. The aim of this summary is to view Candida infections from a different perspective and to give an overview on epidemiology, the range of pathophysiology from colonization to the invasive infections, and its impact on mortality. New therapeutic options will also be discussed and how these relate to current guidelines. Finally, the key issue of the choice of antifungal agents will be evaluated.

  20. A radiolabel release microassay for phagocytic killing of Candida albicans

    International Nuclear Information System (INIS)

    The chromium-51 release technique for quantifying intracellular killing of radiolabelled Candida albicans particles was exploited in a microassay in which murine and human phagocytes acted as effectors under peculiarly simple conditions. At appropriate effector: target ratios and with a 4 h incubation, up to 50% specific chromium release could be detected in the supernatant with no need for opsonization or lysis of phagocytes. This simple microassay permits easy-to-perform, simultaneous testing of a variety of different phagocytes even if only available in limited amounts, and provides an objective measurement of intracellular killing of Candida albicans. (Auth.)

  1. Glucanase Induces Filamentation of the Fungal Pathogen Candida albicans

    OpenAIRE

    Xu, H.; Nobile, CJ; Dongari-Bagtzoglou, A.

    2013-01-01

    Candida albicans is the most common human fungal pathogen. Many organisms, including C. albicans, secrete glucanases under different environmental conditions. Here, we report a novel role for beta-1, 3- glucanase in inducing Candida albicans to form filaments at 22°C and enhancing filamentation at 37°C in nutrient-rich medium. Quorum sensing, the efg1-signaling and cek1 MAP kinase pathways are involved in this process. Our data suggest that the natural antifungal agent beta-glucanase may supp...

  2. Performance of commercial latex agglutination tests for the differentiation of Candida dubliniensis and Candida albicans in routine diagnostics.

    Science.gov (United States)

    Chryssanthou, E; Fernandez, V; Petrini, B

    2007-11-01

    Candida dubliniensis is phenotypically similar to Candida albicans and may therefore be underdiagnosed in the clinical microbiology laboratory. The performance of Bichro-Dubli latex agglutination test for rapid species identification of C. dubliniensis was prospectively evaluated on 111 vaginal and 118 respiratory isolates. These had presumptively been identified as C. albicans/C. dubliniensis by their green colonies on CHROMagar Candida plates. Bichro-Dubli test identifed 2 (1.8%) vaginal and 6 (5.1%) respiratory isolates as C. dubliniensis. The test was also positive for 37 C. dubliniensis control strains characterised by 18S-28S DNA-sequencing. Bichro-Dubli test is thus a sensitive and accurate tool for rapid diagnostics in routine laboratories. PMID:18092961

  3. Growth and acid production of Candida species in human saliva supplemented with glucose.

    Science.gov (United States)

    Samaranayake, L P; Hughes, A; Weetman, D A; MacFarlane, T W

    1986-05-01

    Growth characteristic and acid production of oral isolates of Candida albicans and Candida glabrata in glucose supplemented and glucose-free, pooled, human whole saliva were examined. Both Candida species exhibited sigmoidal growth curves in batch cultures of mixed saliva, supplemented with glucose. The growth of Candida in saliva was accompanied by a rapid decline in pH from 7.5 to 3.2 over 48 h and the major acidic components initiating and sustaining this pH drop were pyruvates and acetates. These acidic metabolites may play an important role in the pathogenesis of oral Candida infections. PMID:3091791

  4. The Spermicidal Compound Nonoxynol-9 Increases Adhesion of Candida Species to Human Epithelial Cells In Vitro

    OpenAIRE

    1990-01-01

    All 25 strains of Candida spp. tested were able to grow in medium supplemented with 25% nonoxynol-9 in vitro. Adhesion of Candida spp. to HeLa cells was found to increase in the presence of 5% nonoxynol-9 (2.2- to 6.6-fold; P less than 0.001) and, to a lesser extent, in 12.5% nonoxynol-9. Adhesion of Candida strains cultured in medium supplemented with nonoxynol-9 varied, with five of six strains of Candida albicans and the single strain of Candida tropicalis demonstrating increases of 1.4 to...

  5. Candida and candidaemia. Susceptibility and epidemiology.

    Science.gov (United States)

    Arendrup, Maiken Cavling

    2013-11-01

    In our part of the world invasive fungal infections include invasive yeast infections with Candida as the absolutely dominating pathogen and invasive mould infections with Aspergillus as the main organism. Yeasts are part of our normal micro-flora and invasive infections arise only when barrier leakage or impaired immune function occurs. On the contrary, moulds are ubiquitous in the nature and environment and their conidia inhaled at a daily basis. Hence invasive mould infections typically arise from the airways whereas invasive yeast infections typically enter the bloodstream causing fungaemia. Candida is by far the most common fungal blood stream pathogen; hence this genus has been the main focus of this thesis. As neither the Danish epidemiology nor the susceptibility of fungal pathogens was well described when we initiated our studies we initially wanted to be able to include animal models in our work. Therefore, a comprehensive animal study was undertaken comparing the virulence in a haematogenous mouse model of eight different Candida species including the five most common ones in human infections (C. albicans, C. glabrata, C. krusei, C. parapsilosis and C. tropicalis and in addition three rarer species C. guilliermondii, C. lusitaniae and C. kefyr). We found remarkable differences in the virulence among these species and were able to group the species according to decreasing virulence in three groups I: C. albicans and C. tropicalis, II: C. glabrata, C. lusitaniae and C. kefyr, and III: C. krusei, C. parapsilosis and C. guilliermondii. Apart from being necessary for our subsequent animal experiments exploring in vivo antifungal susceptibility, these findings also helped us understand at least part of the reason for the differences in the epidemiology and the pitfalls associated with the establishment of genus rather than species specific breakpoints. In example, it was less surprising that C. albicans has been the dominant pathogen and associated with a

  6. Performance comparison of phenotypic and molecular methods for detection and differentiation of Candida albicans and Candida dubliniensis

    Directory of Open Access Journals (Sweden)

    Ahmad Suhail

    2012-09-01

    Full Text Available Abstract Background Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, however, C. dubliniensis has the propensity to develop resistance against azoles more easily. We developed a duplex PCR assay for rapid detection and differentiation of C. albicans from C. dubliniensis for resource-poor settings equipped with basic PCR technology and compared its performance with three phenotypic methods. Methods Duplex PCR was performed on 122 germ tube positive and 12 germ tube negative isolates of Candida species previously identified by assimilation profiles on Vitek 2 ID-YST system. Typical morphologic characteristics on simplified sunflower seed agar (SSA, and reaction with a commercial (Bichro-Dubli latex agglutination test were also performed. The assay was further applied on 239 clinical yeast and yeast-like fungi and results were confirmed by DNA sequencing of internal transcribed spacer (ITS region of rDNA. Results The results of duplex PCR assay for 122 germ tube positive and 12 germ tube negative isolates of Candida species were comparable to their identification by Vitek 2 ID-YST system, colony characteristics on SSA and latex agglutination test. Application of duplex PCR also correctly identified all 148 C. albicans and 50 C. dubliniensis strains among 239 yeast-like fungi. Conclusions The data show that both, duplex PCR and Bichro-Dubli are reliable tests for rapid (within few hours identification of clinical yeast isolates as C. dubliniensis or C. albicans. However, duplex PCR may be applied directly on clinical yeast isolates for their identification as C. dubliniensis or C. albicans as it does not require prior

  7. Daya hambat xylitol dan nistation terhadap pertumbuhan Candida albicans (in vitro) (Inhibition effect of xylitol and nistatin combination on Candida albicans growth (in vitro))

    OpenAIRE

    Sarah Kartimah Djajusman; Udijanto Tedjosasongko; Irmawati Irmawati

    2014-01-01

    Background: The growth of Candida albicans can be controlled by using antifungal such as nystatin. These days we found that using antifungal is not enough to control Candida albicans, we also have to control the intake of sugar by using xylitol. Purpose: Purpose of the study was to determine the optimal inhibitory concentration of xylitol-nystatin in the Candida albicans growth. Methods: This was an in-vitro study using an antimicrobial test of serial dilution with xylitol-nystatin and sucros...

  8. Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV-Infected Patients

    OpenAIRE

    Suelen Balero de Paula; Thais Fernanda Bartelli; Vanessa Di Raimo; Jussevania Pereira Santos; Alexandre Tadachi Morey; Marina Andrea Bosini; Celso Vataru Nakamura; Lucy Megumi Yamauchi; Sueli Fumie Yamada-Ogatta

    2014-01-01

    Most Candida spp. infections are associated with biofilm formation on host surfaces. Cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. The present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity of Candida dubliniensis and Candida tropicalis isolated from the oral cavity of HIV-infected patients. All is...

  9. Anticandidal efficacy of cinnamon oil against planktonic and biofilm cultures of Candida parapsilosis and Candida orthopsilosis.

    Science.gov (United States)

    Pires, Regina Helena; Montanari, Lilian Bueno; Martins, Carlos Henrique G; Zaia, José Eduardo; Almeida, Ana Marisa Fusco; Matsumoto, Marcelo T; Mendes-Giannini, Maria José S

    2011-12-01

    Candida parapsilosis is yeast capable of forming biofilms on medical devices. Novel approaches for the prevention and eradication of the biofilms are desired. This study investigated the anticandidal activity of sixteen essential oils on planktonic and biofilm cultures of C. parapsilosis complex. We used molecular tools, enumeration of colony-forming units, the colourimetric MTT assay, scanning electron microscopy (SEM) and a chequerboard assay coupled with software analyses to evaluate the growth kinetics, architecture, inhibition and reduction in biofilms formed from environmental isolates of the Candida parapsilosis complex; further, we also evaluated whether essential oils would interact synergistically with amphotericin B to increase their anticandidal activities. Of the environmental C. parapsilosis isolates examined, C. parapsilosis and C. orthopsilosis were identified. Biofilm growth on polystyrene substrates peaked within 48 h, after which growth remained relatively stable up to 72 h, when it began to decline. Details of the architectural analysis assessed by SEM showed that C. parapsilosis complex formed less complex biofilms compared with C. albicans biofilms. The most active essential oil was cinnamon oil (CO), which showed anticandidal activity against C. orthopsilosis and C. parapsilosis in both suspension (minimum inhibitory concentration-MIC-250 and 500 μg/ml) and biofilm (minimum biofilm reduction concentration-MBRC-1,000 and 2,000 μg/ml) cultures. CO also inhibited biofilm formation (MBIC) at concentrations above 250 μg/ml for both species tested. However, synergism with amphotericin B was not observed. Thus, CO is a natural anticandidal agent that can be effectively utilised for the control of the yeasts tested. PMID:21761153

  10. Conservación de cepas de Candida utilis en agua destilada estéril

    Directory of Open Access Journals (Sweden)

    Aidín Martínez

    2009-01-01

    Full Text Available Algunos métodos han sido utilizados para el mantenimiento de la colección de levaduras. La elección de uno u otro tiene como objetivo garantizar la estabilidad de los cultivos; teniendo en cuenta las dimensiones y posibilidades económicas de la colección. En este trabajo se empleó la preservación de levaduras en agua destilada estéril por ser un método sencillo, económico, seguro y, capaz de garantizar la viabilidad de los cultivos por un período prolongado. Se conservaron 8 cepas de Candida utilis y se aseguró el 100% de supervivencia de las mismas en dicho sustrato durante tres años, a la vez que no se observaron cambios en las características fisiológicas y morfológicas de los cultivos. Palabras clave: preservación, levaduras, agua destilada.

  11. In Vitro Antifungal Activity of Various Persian Cultivars of Punica granatum L. Extracts Against Candida species

    Directory of Open Access Journals (Sweden)

    Bassiri-Jahromi

    2015-08-01

    Full Text Available Background Resistance of Candida species to antifungal agents has potentially serious implications for management of infections. Candida species are now the fourth most common organisms isolated from hospitalized patients. Prevention and control of these infections will require new antimicrobial agents. Plant-derived antifungal agents have always been a source of novel therapeutics. Objectives The aim of this study was to investigate the antifungal effect of pomegranate peel and pulp extracts against Candida species. Materials and Methods Pomegranate pulp and peel were dried and powdered separately. The dried powders were extracted using a soxhlet extractor. The antifungal effect of pomegranate peel and pulp extracts were determined in vitro by using the minimum inhibitory concentration (MIC against five standard species, including Candida albicans (ATCC 10231, Candida parapsilosis (ATCC 22019, Candida tropicalis (ATCC 750, Candida glabrata (PTCC 5297 and Candida krusei (PTCC 5295. Results Maximum inhibitions were attributed to peel extract of the pomegranate cultivar against Candida species. The greatest antifungal inhibition among the eight different cultivars was observed for sour malas, sour white peel and sour summer extracts respectively, against the five Candida strains. The antifungal activity of pulp extracts against Candida species was somewhat negative. Conclusions Our work suggested that pomegranate (Punica granatum L. peel has potential antifungal activity against Candidiasis, and it is an attractive option for the development of new management strategies for candidiasis.

  12. Supressão da resposta imunitária humoral causada pela citrinina Humoral immunosupression caused by citrinin

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    C.A. Carvalho

    2005-04-01

    Full Text Available Avaliou-se o efeito imunotóxico causado por exposição a baixas doses de citrinina (2,5mg kg-1 em camundongos albinos expostos à micotoxina antes (n=15, durante (n=15 e após (n=15 a imunização com antígeno inerte, representado por eritrócitos de carneiro - sheep red blood cells (SRBC. Quinze camundongos foram usados como controle (não intoxicados. Sete dias após o tratamento, os animais foram sangrados e os títulos de anticorpos anti-SRBC e de complemento foram determinados. A citrinina diminuiu os títulos de anticorpos primários em todos os grupos intoxicados. A intoxicação antes e após a imunização provocou diminuição em 87,5% nos títulos médios de anticorpos específicos. A exposição simultânea à imunização gerou diminuição de 75%. Houve acentuada redução nos níveis de complemento circulante, detectada nos animais previamente intoxicados (93,8%, ou intoxicados juntamente com a imunização (87,5%.The immunotoxic effect caused by citrinin was evaluated in albino mice exposed to a single dose of 2.5mg.kg-1 before (n=15, concomitantly (n=15 and after (n=15 immunization with an inert antigen represented by sheep red blood cells (SRBC. The animals were bleed seven days following the mycotoxin exposure for antibodies anti-SRBC and complement titration and compared to results obtained from non-exposed controls (n=15. It was detected a decreasing antibodies titration in all the intoxicated animals. Those animals that received citrinin before and after SRBC sensitization equally presented a lowering of 87.5% on the primary antibodies level. The exposure to the mycotoxin simultaneously to the SRBC sensitization caused a decrease equivalent to 75%. A remarkable effect was also demonstrated for the circulating complement in both groups of animals, with a decrease of 87.5% on those intoxicated before and of 93.8% on those treated concomitantly to the SRBC sensitization.

  13. Erratum: Evaluation of CHROMagar Candida, VITEK2 YST and VITEK® MS for identification of Candida strains isolated from blood cultures.

    Science.gov (United States)

    Mutlu Sariguzel, Fatma; Berk, Elife; Nedret Koc, Ayse; Sav, Hafize; Aydemir, Gonca

    2016-03-01

    Erratum Following publication of the original article (Infez Med. volume 23, issue 4, pages 318-322, year 2015) we became aware of the following errors which we wish to correct. These corrections have no impact over the study results, their interpretation or conclusions. Title The correct title is the following: Evaluation of chromagenic agar, VITEK2 YST and VITEK® MS for identification of Candida strains isolated from blood cultures Text In the whole text CHROMOMagar Candida shoul be read as chromogenic agar. PMID:27031906

  14. Mecanismos fisiopatogênicos e diagnóstico laboratorial da infecção causada pela Entamoeba histolytica Physiopathogenic mechanisms and laboratorial diagnosis of Entamoeba histolytica infection

    Directory of Open Access Journals (Sweden)

    Fred Luciano Neves Santos

    2008-08-01

    Full Text Available A amebíase é a segunda causa de morte entre as doenças parasitárias no mundo. Seu agente etiológico é o protozoário Entamoeba histolytica, que através da secreção de proteinases é capazes de destruir o tecido hospedeiro, matando as células-alvo por contato e fagocitando eritrócitos. Dessa forma, os trofozoítos invadem a mucosa intestinal, provocando a colite amebiana. Em alguns casos atravessam a mucosa e, através da circulação porta, chegam ao fígado, onde causam necrose constituída por poucos trofozoítos rodeados de hepatócitos mortos e debris celulares liquefeitos. Essa invasão está diretamente relacionada com a capacidade de síntese e a secreção de moléculas responsáveis pela virulência dos trofozoítos, como os amebaporos, as lectinas e as cisteína proteinases. O diagnóstico da infecção causada pelo patógeno é rotineiramente realizado através da microscopia óptica de amostras frescas ou espécimes fixados. Entretanto essa metodologia apresenta limitações, sendo incapaz de distinguir as espécies pertencentes ao complexo E. histolytica/E. dispar. A pesquisa de coproantígenos e a reação em cadeia da polimerase (PCR têm sido utilizadas para diferenciação desses protozoários em amostras fecais. No entanto, estudos mais aprofundados são necessários para maior compreensão sobre a relação parasita/hospedeiro, a proteômica e a genômica do protozoário, o desenvolvimento de vacinas e a real prevalência dessa infecção no Brasil e no mundo.Amebiasis is the second cause of death among parasitary diseases in the world. Its etiologic agent is the protozoan Entamoeba histolytica, which destroys the host tissue by means of the secretion of proteinases, kills the target-cells by contact and phagocytizes erythrocytes. Accordingly, the trophozoites invade the intestinal mucosa, what causes amoebaean colitis. In some cases, they pass through the mucosa and reach the liver through the portal system, where

  15. Caracterización de accidentes de trabajo por caída de objetos en empresas del sector hidrocarburos

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    Gloria A. Jaramillo

    2014-12-01

    Full Text Available En una multinacional del sector de hidrocarburos prestadora de servicios de perforación y workover, que opera en las zonas de Tolima y Santander Colombia, se pudo desarrollar una investigación con el objetivo de describir la situación de accidentalidad causada por caída de objetos que se presenta en el área de taladros de perforación. La información se recolectó de los reportes de accidentes e incidentes que reposaban en la base de operaciones en Sopó entre el periodo 2010 y 2011. Al analizar los hallazgos se pudo evidenciar que son cuatro los agentes causales más significativos, dentro de los cuales están los denominados Golpes por objetos, ocupando un 68%; atrapado por o entre un objeto con 41%; caída de personas con un 30% y caída de objetos con 21 %. Este último hallazgo se deriva por la actividad propia de la perforación por la manipulación de llaves hidráulicas, objetos adicionales de acero, martillos, entre otros y su causa básica fue la falta de supervisión, liderazgo y/o planificación con 39%, seguida de procedimientos de trabajo inadecuados con el 22% y el mantenimiento inadecuado con el 11%, lo que concluye que en su mayoría son errores humanos.

  16. Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate

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    Débora Danielle Virgínio da Silva

    2007-03-01

    Full Text Available The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5. Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g-1 and volumetric productivity (0.53 g l-1.h-1 were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%.O efeito da glicose na bioconversão de xilose em xilitol por Candida guilliermondii foi avaliado em hidrolisado hemicelulósico de bagaço de cana com diferentes relações glicose:xilose (1:25, 1:12, 1:5 and 1:2,5. Sob as condições experimentais, o aumento da relação glicose:xilose favoreceu a assimilação da xilose presente no hidrolisado, resultando em aumento da biomassa celular e aumento da formação de xilitol e dos sub-produtos glicerol e etanol. Os valores máximos do fator de conversão de xilose em xilitol (0,59 g g-1 e da produtividade volumétrica de xilitol (0,53 g l-1.h-1 foram obtidos com a relação glicose:xilose 1:5, resultando na maior eficiência de conversão (64,3%.

  17. DAYA HAMBAT EKSTRAK BUAH MENGKUDU TERHADAP PERTUMBUHAN CANDIDA ALBICANS

    OpenAIRE

    Muhammad, Ilyas

    2008-01-01

    Mengkudu mengandung saponin, flavonoid, minyak atsiri dan alkaloid yang dinyatakan sebagai antibakteri dan antijamur. Penelitian ini merupakan Eksperimen Laboratories dengan rancangan Time Series Design menggunakan isolat ???Candida Albicans??? yang telah diremajakan, adapun tempat pelaksanaannya pada Laboratorium Mikrobiologi Fakultas Kedokteran Unhas. Tujuan penelitian ini yaitu untuk mengetahui konsentrasi hambat minimal dan daya hambat sari buah mengkudu berdasakan konsentarsi terhadap...

  18. Molecular and cellular mechanisms that lead to Candida biofilm formation

    NARCIS (Netherlands)

    J.M. ten Cate; F.M. Klis; T. Pereira-Cenci; W. Crielaard; P.W.J. de Groot

    2009-01-01

    Fungal infections in the oral cavity are mainly caused by C. albicans, but other Candida species are also frequently identified. They are increasing in prevalence, especially in denture-wearers and aging people, and may lead to invasive infections, which have a high mortality rate. Attachment to muc

  19. Candida albicans susceptibility to lactoperoxidase-generated hypoiodite

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    Mohamed Ahariz

    2010-08-01

    Full Text Available Mohamed Ahariz1, Philippe Courtois21Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium; 2Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium and UER de Biologie Médicale, Haute Ecole Francisco Ferrer, Brussels, BelgiumAbstract: In vivo, lactoperoxidase produces hypothiocyanite (OSCN- from thiocyanate (SCN- in the presence of hydrogen peroxide (H2O2; in vitro, iodide (I- can be oxidized into hypoiodite (OI- by this enzyme. The aim of this study was to compare in vitro the anti-Candida effect of iodide versus thiocyanate used as lactoperoxidase substrate to prevent Candida biofilms development. Candida albicans ATCC 10231 susceptibility upon both peroxidase systems was tested in three different experimental designs: (i in a liquid culture medium, (ii in an interface model between solid culture medium and gel containing the enzymic systems, (iii in a biofilm model onto titanium and acrylic resin. Yeast growth in liquid medium was monitored by turbidimetry at 600 nm. Material-adherent yeast biomass was evaluated by the tetrazolium salt MTT method. The iodide-peroxidase system has been shown to inhibit Candida biofilm formation at lower substrate concentrations (~200 fold less H2O2 donor and for longer incubation periods than the thiocyanate-peroxidase system. In conclusion, efficiency of lactoperoxidase-generated OI- to prevent C. albicans biofilm development allows refining iodine antifungal use in ex vivo conditions.Keywords: denture, iodide, oral, peroxidase, saliva, titanium

  20. Is Candida stellatoidea disappearing from the vaginal mucosa?

    OpenAIRE

    Kwon-Chung, K J; Wickes, B L; Salkin, I F; Kotz, H L; Sobel, J D

    1990-01-01

    A total of 681 vaginal isolates of germ tube-positive or germ tube-untested white, yeastlike fungi obtained from patients in various cities of the United States were tested for the presence of Candida stellatoidea (type I). Only 1 of the 681 isolates was identified as C. stellatoidea.