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Sample records for catfish pangasianodon hypophthalmus

  1. The effect of environmental hypercapnia and size on nitrite toxicity in the striped catfish (Pangasianodon hypophthalmus)

    DEFF Research Database (Denmark)

    Hvas, Malthe; Damsgaard, Christian; Gam, Le Thi Hong

    2016-01-01

    Striped catfish (Pangasianodon hypophthalmus) are farmed intensively at high stocking densities in Vietnam where they are likely to encounter environmental hypercapnia as well as occasional high levels of aquatic nitrite. Nitrite competes with Cl- for uptake at the branchial HCO3-/Cl- exchanger, ...... the ambient concentration, while small fish did not. Small P. hypophthalmus instead had significantly higher plasma [nitrate], and haemoglobin concentrations, revealing greater capacity for detoxifying nitrite by oxidising it to nitrate.......Striped catfish (Pangasianodon hypophthalmus) are farmed intensively at high stocking densities in Vietnam where they are likely to encounter environmental hypercapnia as well as occasional high levels of aquatic nitrite. Nitrite competes with Cl- for uptake at the branchial HCO3-/Cl- exchanger...... to a reduced nitrite uptake. To assess the effect of hypercapnia on nitrite uptake, fish were cannulated in the dorsal aorta, allowing repeated blood sampling for measurements of haemoglobin derivatives, plasma ions and acid-base status during exposure to 0.9 mM nitrite alone and in combination with acute...

  2. Hypoxia tolerance and partitioning of bimodal respiration in the striped catfish (Pangasianodon hypophthalmus)

    DEFF Research Database (Denmark)

    Lefevre, Sjannie; Huong, Do Thi Thanh; Wang, Tobias

    2011-01-01

    Air-breathing fish are common in the tropics, and their importance in Asian aquaculture is increasing, but the respiratory physiology of some of the key species such as the striped catfish, Pangasianodon hypophthalmus Sauvage 1878 is unstudied. P. hypophthalmus is an interesting species...... to air-breathe. The possibility of reducing air-breathing frequency may be energetically beneficial for the fish, leaving more of the aerobic scope for growth and other activities, due to the proposed energetic costs of surfacing behavior....... as it appears to possess both well-developed gills and a modified swim bladder that functions as an air-breathing organ indicating a high capacity for both aquatic and aerial respiration. Using newly developed bimodal intermittent-closed respirometry, the partitioning of oxygen consumption in normoxia...

  3. Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

    Science.gov (United States)

    Phu, Tran Minh; Phuong, Nguyen Thanh; Scippo, Marie-Louise; Dalsgaard, Anders

    2015-01-01

    Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus) farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials) commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatography Mass Spectrometry was used to analyze concentration of sulfonamides, trimethoprim, amoxicillin, cefalexin and ciprofloxacin whereas concentrations of florfenicol and doxycycline were analyzed by High Performance Liquid Chromatography with UV detection. Results revealed that only 4/11 products with a single antimicrobial and 2/10 products with a mixture of antimicrobials contained active substances within ±10% of the concentration declared on the product label. Two products with antimicrobial mixtures did not contain any of the declared antimicrobials. Comparing two batches, analysis of 11 products revealed that only one product contained a concentration of active compound that varied with less than 10% in both batches. Several product labels provided inadequate information on how to calculate therapeutic dosage and further stated withdrawal time despite lack of pharmacokinetic data on the antimicrobials in catfish. There is an urgent need to strengthen approval procedures and in particular regularly to monitor the quality of antimicrobials used in Vietnamese aquaculture. PMID:25897517

  4. Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus Aquaculture in Vietnam.

    Directory of Open Access Journals (Sweden)

    Tran Minh Phu

    Full Text Available Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatography Mass Spectrometry was used to analyze concentration of sulfonamides, trimethoprim, amoxicillin, cefalexin and ciprofloxacin whereas concentrations of florfenicol and doxycycline were analyzed by High Performance Liquid Chromatography with UV detection. Results revealed that only 4/11 products with a single antimicrobial and 2/10 products with a mixture of antimicrobials contained active substances within ±10% of the concentration declared on the product label. Two products with antimicrobial mixtures did not contain any of the declared antimicrobials. Comparing two batches, analysis of 11 products revealed that only one product contained a concentration of active compound that varied with less than 10% in both batches. Several product labels provided inadequate information on how to calculate therapeutic dosage and further stated withdrawal time despite lack of pharmacokinetic data on the antimicrobials in catfish. There is an urgent need to strengthen approval procedures and in particular regularly to monitor the quality of antimicrobials used in Vietnamese aquaculture.

  5. Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus

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    D. Wahjuningrum

    2007-01-01

    Full Text Available Motile Aeromonad Septicaemia (MAS disease is one of bacterial disease frequently infecting freshwater fishes including patin catfish Pangasianodon hypophthalmus.  This study was performed to determine antimicrobial of Phaleria macrocarpa (PM and its potency against MAS disease caused by Aeromonas hydrophila.  The in vitro susceptibility test was performed by pour plate methods at the dosages of 2, 4, 6, 8, and 10 g/l PM. At the in vivo test, fish were fed with the addition of PM into the diet at a dosage of 6, 12, and 18 g/l and 0 g/l as a control for 8 days. At ninth day, fish were infected with A.hydrophila. For seven days after infection the clinical signs and blood pictures were observed. The in vitro test indicated that PM had an antibacterial effect to A.hydrophila at the dosage of 6 g/l. Addition of PM in the diet for 8 days increased haemoglobine. The results showed that lowest clinical sign and smallest number of in fected fish was found at dosage of 12 g/l PM. PM can be used as a preventive method for MAS. Keywords:  Phaleria macrocarpa, antibacterial, "patin", MAS disease, Aeromonas hydrophila   Abstrak Penyakit MAS (Motile Aeromonad Septicaemia merupakan penyakit bakterial yang banyak menyerang ikan-ikan air tawar termasuk patin Pangasianodon hypophthalmus. Penelitian ini bertujuan untuk melihat kemampuan antibakteri dari mahkota dewa (MD Phaleria macrocarpa terhadap Aeromonas hydrophila penyebab penyakit MAS dan potensinya dalam pencegahan penyakit ini.  Pada uji in vitro dilakukan pengujian aktivitas antibakteri MD terhadap A. hydrophila dengan metode hitungan cawan pada dosis MD 2, 4, 6, 8, dan 10 g/l. Pada uji in vivo, ikan uji diberi pakan yang dicampur MD dengan dosis berbeda yaitu 0 g/l (kontrol +, 6, 12, dan 18 g/l, selama 8 hari. Pada hari kesembilan ikan disuntik dengan A. hydrophila dan pengamatan dilanjutkan selama 7 hari, meliputi pengamatan gejala klinis dan gambaran darah.  Hasil penelitian menunjukkan bahwa MD

  6. Record of Iridescent shark catfish Pangasianodon hypophthalmus Sauvage, 1878 (Siluriformes: Pangasiidae from Madampa-Lake in Southwest Sri Lanka

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    Hareschandra Bandula Jayaneththi

    2015-12-01

    Full Text Available An exotic catfish was recorded at Madampa Lake in Southwest coastal zone of Sri Lanka during an ichthyofaunal survey carried out from 1st of April 2009 to 20th August 2010. The species is identified as Pangasianodon hypophthalmus, which has been described as a potentially problematic species on native fishes in some neighbouring countries of the region. This species naturally occurs in the Mekong River in South-East Asia. The observation of P. hypophthalmus is a novel record in wild habitats of Sri Lanka, where its introduction is probably an accidental release from the ornamental fish trade, but no substantial information is detected as a proof of invasion or a naturalisation of the current population.

  7. Gill remodelling and growth rate of striped catfish Pangasianodon hypophthalmus under impact of hypoxia and temperature

    DEFF Research Database (Denmark)

    Phuong, Le My; Huong, Do Thi Thanh; Nyengaard, Jens Randel

    2017-01-01

    Gill morphometric and gill plasticity of the air-breathing striped catfish (Pangasianodon hypophthalmus) exposed to different temperatures (present day 27 °C and future 33 °C) and different air saturation levels (92% and 35%) during 6 weeks were investigated using vertical sections to estimate...... the respiratory lamellae surface areas, harmonic mean barrier thicknesses, and gill component volumes. Gill respiratory surface area (SA) and harmonic mean water - blood barrier thicknesses (HM) of the fish were strongly affected by both environmental temperature and oxygen level. Thus initial values for 27 °C...... normoxic fish (12.4 ± 0.8 g) were 211.8 ± 21.6 mm2 g−1 and 1.67 ± 0.12 μm for SA and HM respectively. After 5 weeks in same conditions or in the combinations of 33 °C and/or PO2 of 55 mm Hg, this initial surface area scaled allometrically with size for the 33 °C hypoxic group, whereas branchial SA...

  8. Study on preparation and effect of oligoβ-glucan and oligochitosan on immune stimulation white patches in the internal organs disease on Tra catfish (Pangasianodon hypophthalmus)

    International Nuclear Information System (INIS)

    Nguyen Ngoc Duy; Dang Van Phu; Nguyen Thi Kim Lan; Nguyen Quoc Hien; Pham Duy Hai

    2015-01-01

    Oligoβ-glucan and oligochitosan were prepared by gamma Co-60 irradiation of β-glucan/H_2O_2 and chitosan/H_2O_2 solution. The efficiency of the degradation process was determined by gel permeation chromatography (GPC) method. Results showed that the Mw decreased with increasing concentration of H_2O_2 and doses. For oligoβ-glucan, Mw reduced from 56.7 kDa to 7.1 kDa when β-glucan 10%/H_2O_2 1% solution was irradiated at 14 kGy. For oligochitosan, Mw reduced from 45.5 kDa to 5.0 kDa when chitosan 5%/H_2O_2 0.5% solution was irradiated at 21 kGy. Tra catfish (Pangasianodon hypophthalmus) was fed with oligoβ-glucan and oligochitosan in various concentrations of 0, 50, 100, and 200 mg/kg feed for 45 days and then was challenged with Edwardsiella ictaluri bacteria to investigate immune stimulation effect against white patches in the internal organs disease. The results indicated that oligoβ-glucan and oligochitosan exhibited good immune stimulation effect with optimum concentration of 100 mg/kg feed. Survival rate of Tra catfishes fed with oligochitosan and oligoβ-glucan is 47.62 ± 1.96% and 46.67 ± 2.58%, respectively. In addition, the mixture of oligochitosan 50 mg/kg + oligo?-glucan 50 mg/kg showed the highest survival rate (62.22 ± 1.96%). (author)

  9. Rigor index, fillet yield and proximate composition of cultured striped catfish (Pangasianodon hypophthalmus for its suitability in processing industries in Bangladesh

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    Salma Noor-E Islami

    2014-12-01

    Full Text Available Rigor-index in market-size striped catfish (Pangasianodon hypophthalmus, locally called Thai-Pangas was determined to assess fillet yield for production of value-added products. In whole fish, rigor started within 1 hr after death under both iced and room temperature conditions while rigor-index reached a maximum of 72.23% within 8 hr and 85.5% within 5 hr at room temperature and iced condition, respectively, which was fully relaxed after 22 hr under both storage conditions. Post-mortem muscle pH decreased to 6.8 after 2 hr, 6.2 after 8 hr and sharp increase to 6.9 after 9 hr. There was a positive correlation between rigor progress and pH shift in fish fillets. Hand filleting was done post-rigor and fillet yield experiment showed 50.4±2.1% fillet, 8.0±0.2% viscera, 8.0±1.3% skin and 32.0±3.2% carcass could be obtained from Thai-Pangas. Proximate composition analysis of four regions of Thai-Pangas viz., head region, middle region, tail region and viscera revealed moisture 78.36%, 81.14%, 81.45% and 57.33%; protein 15.83%, 15.97%, 16.14% and 17.20%; lipid 4.61%, 1.82%, 1.32% and 24.31% and ash 1.09%, 0.96%, 0.95% and 0.86%, respectively indicating suitability of Thai-Pangas for production of value-added products such as fish fillets.

  10. Effect of ingredient particle sizes and dietary viscosity on digestion and faecal waste of striped catfish (Pangasianodon hypophthalmus)

    NARCIS (Netherlands)

    Tran, Tu; Hien, T.T.T.; Bosma, R.H.; Heinsbroek, L.T.N.; Verreth, J.A.J.; Schrama, J.W.

    2017-01-01

    The ingredients' particle size and dietary viscosity may alter digestion, performance and faecal waste management of fish. This study aimed to assess the effect of grinding screen sizes of feed ingredients and dietary viscosity on digestibility, faecal waste and performance of striped catfish

  11. Effect of ingredient particle sizes and dietary viscosity on digestion and faecal waste of striped catfish (Pangasianodon hypophthalmus)

    NARCIS (Netherlands)

    Tran, Tu; Hien, T.T.T.; Bosma, R.H.; Heinsbroek, L.T.N.; Verreth, J.A.J.; Schrama, J.W.

    2018-01-01

    The ingredients' particle size and dietary viscosity may alter digestion, performance and faecal waste management of fish. This study aimed to assess the effect of grinding screen sizes of feed ingredients and dietary viscosity on digestibility, faecal waste and performance of striped catfish

  12. Two-year intervention trial to control of fish-borne zoonotic trematodes in giant gourami (Osphronemus goramy) and striped catfish (Pangasianodon hypophthalmus) in nursery ponds in the Mekong Delta, Vietnam.

    Science.gov (United States)

    Madsen, H; Thien, P C; Nga, H T N; Clausen, J H; Dalsgaard, A; Murrell, K D

    2015-12-01

    Fish-borne zoonotic trematode parasites (FZT) pose a food safety and public health problem in Vietnam. The transmission cycle is complex as domestic animals, especially dogs, cats, fish-eating birds and pigs together with humans serve as reservoir hosts and contribute to FZT egg contamination of aquaculture ponds and the environment. This intervention trial was conducted to determine the effectiveness of various on-farm interventions, including reduction in FZT egg contamination through treatment of infected people and domestic animals, reduction in snail density through mud removal from aquaculture ponds prior to fish stocking, and various other measures in reducing FZT infection in juvenile striped catfish (Pangasianodon hypophthalmus) and giant gourami (Osphronemus goramy). Interventions were implemented on 5 farms for each fish species during production cycles in 2009 and 2010 while 5 similar farms for each species served as control. For both fish species, both prevalence and intensity of infection did not differ significantly between intervention and non-intervention farms prior to the interventions. The interventions significantly reduced both prevalence and intensity of FZT infection in the juvenile fish compared to control ponds. For giant gourami, odds of infection in intervention ponds was 0.13 (95% CL: 0.09-0.20; p<0.001) of that in non-intervention ponds after the 2009 trial and 0.07 (0.03-0.14; p<0.001) after the 2010 trial. For striped catfish, these figures were 0.17 (0.08-0.35; p<0.001) after the 2009 trial while after the 2010 trial all ponds with interventions were free from infection. Metacercariae intensity (no. of metacercariae/fish) in giant gourami from intervention ponds was 0.16 (0.11-0.23; p<0.001) of that in fish from non-intervention ponds after the 2009 trial and 0.07 (0.04-0.15; p<0.001) after the 2010 trial; for striped catfish these figures were 0.18 (0.09-0.36; p<0.001) and 0.00 (confidence limits not estimated), respectively. The

  13. A Decision Tree Analysis to Support Potential Climate Change Adaptations of Striped Catfish (Pangasianodon hypophthalmus Sauvage) Farming in the Mekong Delta, Vietnam

    NARCIS (Netherlands)

    Nguyen, L.A.; Verreth, J.A.J.; Leemans, H.B.J.; Bosma, R.H.; Silva, De S.

    2016-01-01

    This study uses the decision tree framework to analyse possible climate change impact adaptation options for pangasius (Pangasianodon hypopthalmus Sauvage) farming in the Mekong Delta. Here we present the risks for impacts and the farmers' autonomous and planned public adaptation by using primary

  14. Anoxia and Acidosis Tolerance of the Heart in an Air-Breathing Fish (Pangasianodon hypophthalmus).

    Science.gov (United States)

    Joyce, William; Gesser, Hans; Bayley, Mark; Wang, Tobias

    2015-01-01

    Air breathing has evolved repeatedly in fishes and may protect the heart during stress. We investigated myocardial performance in the air-breathing catfish Pangasianodon hypophthalmus, a species that can withstand prolonged exposure to severe hypoxia and acidosis. Isometric ventricular preparations were exposed to anoxia, lactic acidosis, hypercapnic acidosis, and combinations of these treatments. Ventricular preparations were remarkably tolerant to anoxia, exhibiting an inotropic reduction of only 40%, which fully recovered during reoxygenation. Myocardial anoxia tolerance was unaffected by physiologically relevant elevations of bicarbonate concentration, in contrast to previous results in other fishes. Both lactic acidosis (5 mM; pH 7.10) and hypercapnic acidosis (10% CO2; pH 6.70) elicited a biphasic response, with an initial and transient decrease in force followed by overcompensation above control values. Spongy myocardial preparations were significantly more tolerant to hypercapnic acidosis than compact myocardial preparations. While ventricular preparations were tolerant to the isolated effects of anoxia and acidosis, their combination severely impaired myocardial performance and contraction kinetics. This suggests that air breathing may be a particularly important myocardial oxygen source during combined anoxia and acidosis, which may occur during exercise or environmental stress.

  15. BIOMETRIC ANALYSIS OF THE ARTIFICIAL HYBRIDIZATION BETWEEN Pangasius djambal BLEEKER, 1846 AND Pangasianodon hypophthalmus SAUVAGE, 1878

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    Rudhy Gustiano

    2016-10-01

    Full Text Available It is really important, since the possible use of these pangasiid hybrids in aquaculture faces the problem of potential impact on wild population. Therefore, it is urgently needed to provide quick identification tools in the field. This study investigated morphological characters of Pangasius djambal and Pangasianodon hypophthalmus and their hybrids. A detailed morphological analysis using 32 morphometric measurements and five meristic counts was done on the hybridization of P. djambal and P. hypophthalmus. Morphometric analysis and meristic counts showed that the reciprocal hybrids have intermediate characters except for gill raker number in which lower than that of parental species. In general, the hybrids have tendency to be like P. hypophthalmus rather than P. djambal. The only typical character of P. djambal appeared on hybrids is teeth shape, both vomerine and palatine. It is clearly defined that the true hybrids have seven pelvic fin rays.

  16. Ventilatory and Cardiovascular Regulation in the Air-Breathing Fish Pangasianodon Hypophthalmus

    DEFF Research Database (Denmark)

    Thomsen, Mikkel; Wang, Tobias; Bayley, Mark

    The air-breathing fish Pangasianodon hypophthalmus is abundant in the Mekong river system where it is also intensively cultured. In contrast to most other air-breathing fishes it has well developed gills as well as a highly traberculated swim bladder with a large surface area used for air-breathing...... systems provide information on when gill ventilation is insufficient for oxygen uptake and hence initiate air-breathing. Here we investigate the ventilatory and cardiovascular responses to changes in either in the external media or internally in the blood in resting fish. We found ventilation in P....... Its native waters have been shown to be periodically strongly hypoxic and hypercarbic, forcing P. hypophthalmus to switch from exclusively branchial ventilation to air-breathing to maintain its aerobic metabolism. This ability to switch respiratory media demands that the oxygen- and CO¬2 sensory...

  17. High capacity for extracellular acid-base regulation in the air-breathing fish Pangasianodon hypophthalmus.

    Science.gov (United States)

    Damsgaard, Christian; Gam, Le Thi Hong; Tuong, Dang Diem; Thinh, Phan Vinh; Huong Thanh, Do Thi; Wang, Tobias; Bayley, Mark

    2015-05-01

    The evolution of accessory air-breathing structures is typically associated with reduction of the gills, although branchial ion transport remains pivotal for acid-base and ion regulation. Therefore, air-breathing fishes are believed to have a low capacity for extracellular pH regulation during a respiratory acidosis. In the present study, we investigated acid-base regulation during hypercapnia in the air-breathing fish Pangasianodon hypophthalmus in normoxic and hypoxic water at 28-30°C. Contrary to previous studies, we show that this air-breathing fish has a pronounced ability to regulate extracellular pH (pHe) during hypercapnia, with complete metabolic compensation of pHe within 72 h of exposure to hypoxic hypercapnia with CO2 levels above 34 mmHg. The high capacity for pHe regulation relies on a pronounced ability to increase levels of HCO3(-) in the plasma. Our study illustrates the diversity in the physiology of air-breathing fishes, such that generalizations across phylogenies may be difficult. © 2015. Published by The Company of Biologists Ltd.

  18. STRUKTUR KOMUNITAS DAN RELUNG MAKANAN IKAN PASCA INTRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus DI WADUK MALAHAYU, KABUPATEN BREBES

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    Kunto Purnomo

    2016-06-01

    Full Text Available Studi tentang struktur komunitas ikan dan pembagian sumber daya pakan ikan pasca introduksi ikan patin siam (Pangasianodon hypophthalmus di Waduk Malahayu (620 ha dilakukan dengan tujuan untuk mengetahui komposisi jenis ikan, preferensi makanan, dan luas relung tiap jenis ikan. Penelitian dilaksanakan dengan menggunakan metode survei pada bulan Agustus sampai Nopember 2009 dan bulan Maret sampai Oktober 2010. Hasil penelitian menunjukkan bahwa struktur komunitas ikan terdiri atas 13 jenis ikan, yang didominansi oleh ikan nila (Oreochromis niloticus, udang (Macrobrachium sp., dan gabus (Channa striata. Jenis-jenis sumber daya pakan yang dimanfaatkan oleh ikan adalah fitoplankton (20%, detritus (19%, zooplankton (17%, insekta (11%, tumbuhan air (9%, ikan (9%, udang (9%, dan moluska (6%. Ikan patin siam, mujair (Oreochromis mossambicus, dan beunteur (Puntius binotatus bersifat generalis karena mampu memanfaatkan semua sumber daya pakan alami yang tersedia. Ikan sili (Macrognathus aculeatus dan keting (Mystus nigriceps lebih bersifat spesialis karena hanya memanfaatkan insekta sebagai makanan utamanya. Peluang kompetisi antara ikan patin siam (diintroduksikan tahun 2009 dan ikan nila relatif kecil sebab sumber daya pakan utamanya berbeda, yaitu ikan patin siam memanfaatkan moluska sebagai makanan utamanya sedangkan ikan nila sebagian memanfaatkan fitoplankton. Hasil tangkapan ikan di waduk ini berkisar antara 34,3-1.323,1 ton/tahun dengan rata-rata 157,3 ton/tahun.   Malahayu Reservoir located in Brebes Regency, was impounded in 1930, with a surface area of 620 hectares, a mean water depth of 8 m. Its main function are flood control and irrigation. Study on fish community structure and food resource partitioning of fishes in Malahayu Reservoir were conducted from August to November 2009 and March to October 2010. The aim of the study was to evaluate the existing condition of fish resources, with emphasis on species composition, food preferency, and

  19. Increasing Thai Catfish's Immunity (Pangasius hypophthalmus Fowler Using Ascorbic Acid

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    , Ilmiah

    2007-08-01

    Full Text Available ABSTRACTAn experiment to determine Thai catfish's (Pangasius hypophthalmus Fowler immunity was carried out using different levels of ascorbic acid (0, 1.000, 2.000 and 3.000 mg/kg feed.  Fish of 15-20 g in weight were kept in aquaria for 6 weeks with density of 15 fish/aquaria.  Feed was given at 5-10 % of total biomass with frequency of three times a day.  The blood sampling was taken every week and the challenge test with Aeromonas hydrophila (106cells/mm3 injection intramuscular was done on the 30th day.   The result of this experiment shown that feed with ascorbic acid of 2.000 mg/kg was elevated the cellular responses such as: leucocyte total (34.850 cels/mm3, differential of leucocyte (lymphocyte: 72,2%, monocyte: 8,0%, neutrophyl: 7,8%, phagocytic index (13% and humoral response (titre antibody: 0.829 serum aglutination unit, which at the same time proves high level of survival rate against the artificial injection using A. hydrophila. Key words :  Ascorbic acid, fish immunity, Thai catfish, Pangasius hypophthalmus.   ABSTRAK Suatu penelitian telah dilakukan di laboratorium dengan menggunakan ikan jambal Siam (Pangasius hypophthalmus Fowler untuk melihat tingkat kekebalan ikan dengan menambahkan vitamin C pada pakan (0, 1.000, 2.000 dan 3.000 mg/kg pakan.  Ikan jambal Siam ukuran 15-20 g dipelihara dalam aquarium selama 6 minggu dengan kepadatan 15 ekor/wadah.  Pemberian pakan dilakukan 3 kali sehari sebanyak 5-10% dari bobot biomasa, pengambilan contoh darah dilakukan setiap minggu dan uji tantang dilakukan pada hari ke-30 dengan bakteri Aeromonas hydrophila (106 sel/mm3 secara intramuskular.  Hasil penelitian menunjukkan bahwa penambahan vitamin C sebanyak 2.000 mg/kg pakan menyebabkan meningkatnya respon seluler antara lain: total lekosit (34.850 sel/mm3, jenis lekosit (limfosit: 72,2%, monosit: 8,0%, netrofil: 7,7% dan trombosit: 17,6% indeks fagositik 13% dan respon humoral (titer antibodi: 0,829 unit serum aglutinasi

  20. Embryonic and larvae development of reciprocal crosses between Pangasianodon hypophthalmus (Sauvage, 1878 and Clarias gariepinus (Burchell, 1822

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    V.T. Okomoda

    2017-12-01

    Full Text Available The egg and larval development of reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878 and Clarias gariepinus (Burchell, 1822 were studied under laboratory conditions. Crosses between ♀C. gariepinus × ♂P. hypophthalmus (Clariothalmus and ♀P. hypophthalmus × ♂C. gariepinus (Pangapinus had embryonic stages similar to those of the pure sib, however, unequal cell cleavages were notable in the early development of both crosses, hence, leading to different forms of deformities. The critical stage where highest mortality occurred during the embryogenesis was the somite (21.68% and hatching (48.1% stages (respectively for the Clariothalmus and Pangapinus. However, both crosses produced viable larvae (60.21 vs 13.20% hatchability respectively, which survived (30.3 vs 2.1% respectively until the end of the study (2 weeks. The external morphological features of the larvae were completely formed by the 14th day after hatching. The body forms of the crosses at this time were either phenotypic intermediary of the parent species (92% of Clariothalmus or indistinguishable from the female parent (all Pangapinus and 8% of the Clariothalmus. This study thus laid the groundwork for further comparative studies on hybrid performance and characterization.

  1. High affinity and temperature sensitivity of blood oxygen binding in Pangasianodon hypophthalmus due to lack of chloride-hemoglobin allosteric interaction

    DEFF Research Database (Denmark)

    Damsgaard, Christian; Phuong, Le My; Huong, Do Thi Thanh

    2015-01-01

    Air-breathing fishes represent interesting organisms in terms of understanding the physiological changes associated with the terrestrialization of vertebrates, and, further, are of great socio-economic importance for aquaculture in Southeast Asia. To understand how environmental factors......, such as high temperature, affect O2 transport in air-breathing fishes, this study assessed the effects of temperature on O2 binding of blood and Hb in the economically important air-breathing fish Pangasianodon hypophthalmus. To determine blood O2 binding properties, blood was drawn from resting cannulated...

  2. Trails of river monsters: Detecting critically endangered Mekong giant catfish Pangasianodon gigas using environmental DNA

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    Bellemain Eva

    2016-07-01

    Full Text Available Pressures on freshwater biodiversity in Southeast Asia are accelerating, yet the status and conservation needs of many of the region’s iconic fish species are poorly known. The Mekong is highly species diverse and supports four of the six largest freshwater fish globally, three of which, including Mekong giant catfish (Pangasianodon gigas, are Critically Endangered. Emerging environmental DNA (eDNA techniques have potential for monitoring threatened freshwater biodiversity, yet have not been applied in complex and biodiverse tropical ecosystems such as the Mekong. We developed species-specific primers for amplifying Mekong giant catfish DNA. In situ validation demonstrated that the DNA amplification was successful for all samples taken in reservoirs with known presence of Mekong giant catfish independent of fish density. We collected water samples from six deep pools on the Mekong, identified through Local Ecological Knowledge, in Cambodia, Lao PDR, and Thailand. DNA was extracted and amplified from these samples using the designed primers and probes. Mekong giant catfish DNA was detected from one sample from the species’ presumed spawning grounds on the Mekong mainstream, near the border between northern Thailand and Lao PDR. eDNA sampling using species-specific primers has potential for surveying and monitoring poorly known species from complex tropical aquatic environments. However accounting for false absences is likely to be required for the method to function with precision when applied to extremely rare species that are highly dispersed within a large river system. We recommend that such approach be utilised more widely by freshwater conservation practitioners for specific applications. The method is best suited for baseline biodiversity assessments or to identify and prioritise locations for more rigorous sampling. Our methods are particularly relevant for systems or species with limited baseline data or with physical characteristics

  3. EVALUATION OF HYBRIDIZATION BETWEEN PANGASIUS DJAMBAL BLEEKER 1846 AND PANGASIANODON HYPOPHTHALMUS (SAUVAGE 1878: BIOMETRIC CHARACTERIZATION AND GROWTH ANALYSIS

    Directory of Open Access Journals (Sweden)

    Rudhy Gustiano

    2007-06-01

    Full Text Available Possible use of pangasiid hybrids in aquaculture might generate potential impacts on wild populations. Therefore, rapid identification tools in the field such as growth rate are urgently needed. This study examines morphological characters and growth performance of P. djambal and P. hypophthalmus and their reciprocal hybrids. A detailed morphological study analysed 32 morphometric measurements and 5 meristic counts on hybrids of Pangasius djambal and P. hypophthalmus. Morphometric analysis and meristic counts showed that the reciprocal hybrids have intermediate characters except for gill rakers number which were lower than that of parental species. In general, the hybrids have tendency to be like P. hypophthalmus rather than P. djambal. The only typical character P. djambal appearing in hybrids is teeth shape, both vomerine and palatine. It was shown that the true hybrids have seven pelvic fin rays. Eight months of growth comparison in earthen ponds showed that the hybrids have a better performance for specific growth rate than the parental stock.

  4. Effects of Probiotic Bacillus sp. on Food Convertion and Growth of Catfish Pangasius hypophthalmus

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    Dedi Jusadi

    2007-04-01

    Full Text Available A triplicate experiment was conducted to evaluate the addition of probiotic Bacillus sp. into the diet on feed convertion and growth of catfish Pangasius hypophthalmus. Twenty fish with an initial body weight of 1,85 ± 0,09 g were stocked in a 60-1 aquarium. During rearing period, fish were fed on the diet three times a day at satiation. Prior the feeding, probiotic (contained Bacillus sp. 4,2x106 CFU.ml-1 were added into the diet at a dosage of 0, 5, 15 or 25 ml.kg-1 diet. The probiotic were added once a day at the noon. The results showed that maximum protein retention, lipid retention, growth rate, and minimum feed convertion was found in the group of fish fed on the diet supplemented with 15 ml probiotic kg-1 diet. Irrespective to the dosage of probiotic, food consumption and survival rate of fish were the same among the treatments. Key words : Probiotic. Bacillus sp.. catfish Pangasius hypophthalmus.   ABSTRAK Penelitian ini bertujuan untuk mengetahui dosis yang optimal dari probiotik Bacillus sp. yang ditambahkan pada pakan komersil terhadap konversi pakan dan pertumbuhan benih ikan patin Pangasius hypophthalmus. Dua puluh ekor ikan patin dengan bobot rata-rata 1.85 ± 0,09 g ditebar dalam setiap akuarium frekuensi 50x40x35 cm yang diisi air 60 1. Selama 40 had masa pemeliharaan. ikan diberi pakan buatan berkadar protein 27% dengan frekwensi tiga kali sehari, at satiation. Sebelum diberikan ke ikan, pakan tersebut ditambah produk probiotik (mengandung Bacillus sp. 4,2 x 106 CFU/ml dengan dosis 0, 5, 15 atau 25 ml/kg pakan. Pakan yang mengandung probiotik hanya diberikan sekali setiap hari, yakni pada pukul 13.00. Hasil penelitian menunjukkan bahwa adanya penambahan probiotik dalam pakan sampai dosis 15 ml/kg pakan menyebabkan terjadinya peningkatan retensi protein, retensi lemak dan laju pertumbuhan harian ikan, serta menurunkan konversi pakan. Penambahan probiotik lebih lanjut (25 ml/kg pakan menurunkan kinerja pertumbuhan di atas

  5. Effect of probiotic and sand filtration treatments on water quality and growth of tilapia (Oreochromis niloticus) and pangas (Pangasianodon hypophthalmus) in earthen ponds of southern Bangladesh

    DEFF Research Database (Denmark)

    Mahmud, Sultan; Ali, Mohammad Lokman; Alam, Md Ariful

    2016-01-01

    Effects of water treatment by two probiotic products (PondPlus® and AquaPhoto®) and sand filtration were studied on growth performance of tilapia (Oreochromis niloticus) and pangas (Pangasianodon hypophthalmus) stocked at tilapia:pangas ratio of 5:3 in traditional earthen ponds in Bangladesh....... The fish were stocked at a density of 20,000 fish ha−1 and reared for 7 months. Compared to untreated ponds, treatments of probiotic products or sand filtration in earthen ponds resulted in a higher O2 content, higher water transparency, less ammonium, and fewer cyanobacteria. Weight gain for individual...... tilapia was lowest in the AquaPhoto-treated ponds (177 g), while similar gains (188–194 g) occurred in the other ponds. For pangas, the lowest weight gain (627 g) was obtained in the sand filter-treated ponds, as compared to 690–797 g in the other ponds. Thus, a general positive effect from the treatments...

  6. KERAGAAN PEMIJAHAN BUATAN ANTARA IKAN PATIN SIAM (Pangasianodon hypophthalmus BETINA DAN IKAN PATIN JAMBAL (Pangasius djambal JANTAN DAN IKAN PATIN NASUTUS (Pangasius nasutus JANTAN

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2014-08-01

    Full Text Available Upaya pengembangan budidaya ikan patin jambal (Pangasius djambal dan ikan patin nasutus (P. nasutus sebagai komoditas ekspor ikan patin daging putih sulit direalisasikan, karena kematangan induk-induk betinanya sulit dicapai pada musim kemarau dan keterbatasan fekunditasnya, sehingga produksi massal benihnya terbatas. Salah satu upaya yang dapat dilakukan untuk meningkatkan produktivitas ikan patin daging putih adalah melalui program hibridisasi antara ikan patin siam (Pangasianodon hypophthalmus betina dengan ikan patin jambal jantan (menghasilkan ikan patin hibrida siam x jambal dan atau dengan ikan patin nasutus jantan (menghasilkan ikan patin hibrida siam x nasutus. Potensi budidaya kedua ikan patin hibrida tersebut berkaitan dengan produksi massal benihnya merupakan hal yang penting untuk diketahui. Penelitian ini bertujuan untuk mengevaluasi derajat fertilisasi, derajat penetasan, dan derajat deformitas larva kedua ikan patin hibrida tersebut. Hasil penelitian menunjukkan bahwa derajat fertilisasi ikan patin hibrida siam x nasutus sama (P>0,05 dengan ikan patin siam, ikan patin hibrida siam x jambal dan ikan patin nasutus, sedangkan ikan patin jambal memiliki derajat fertilisasi yang lebih rendah tetapi tidak berbeda (P>0,05 dengan ikan patin nasutus dan ikan patin hibrida siam x jambal. Derajat penetasan ikan patin hibrida siam x nasutus adalah sama (P>0,05 dengan ikan patin siam dan ikan patin hibrida siam x jambal, dan lebih tinggi (P0,05; sedangkan pada ikan patin jambal dan ikan patin nasutus lebih tinggi (P<0,05. Hasil-hasil tersebut menunjukkan bahwa produktivitas (produksi massal benih kedua ikan patin hibrida tersebut tinggi dan sama dengan ikan patin siam, sehingga potensial untuk dikembangkan sebagai ikan budidaya.

  7. Hematologic and plasma chemistry RIs for cultured Striped catfish (Pangasius hypophthalmus) in recirculating aquaculture systems.

    Science.gov (United States)

    Galagarza, Oscar A; Kuhn, David D; Smith, Stephen A; Hrubec, Terry C

    2017-09-01

    Striped catfish (Pangasius hypophthalmus) is a valuable aquaculture fish species produced primarily in Southeast Asia. In the United States, it is bred as an ornamental species. Striped catfish has high productivity and great demand in numerous countries around the world, yet little is known about its normal physiology. The objective of this study was to establish hematologic and blood chemistry RIs for healthy juvenile Striped catfish. Blood samples were collected from 70 Striped catfish raised in recirculating aquaculture systems. Whole blood and plasma samples were analyzed for multiple hematologic and chemistry variables using standard techniques. The RIs for hematology were as follows: PCV 23.5-35.9%, MCV 106.3-156.6 fL, RBC count 1.79-2.75 × 10 6 cells/μL, thrombocytes 26,318-73,333 cells/μL, total WBC count 36,294-94,286 cells/μL, total lymphocytes 18,997-59,998 cells/μL, small lymphocytes 13,763-51,490 cells/μL, large lymphocytes 715-21,200 cells/μL, granulocytes 4504-18,291 cells/μL, and monocytes 0-7549 cells/μL. Plasma chemistry RIs were the following: ALP 32.7-74.6 U/L, AST 20.3-1235.8 U/L, sodium 135.2-147.7 mmol/L, potassium 3.3-5.0 mmol/L, chloride 120.1-133.6 mmol/L, calcium 2.7-3.6 mmol/L, magnesium 0.9-1.3 mmol/L, phosphorous 1.4-2.7 mmol/L, glucose 4.6-7.6 mmol/L, cholesterol 2.8-5.3 mmol/L, total protein 30-42 g/L, albumin 7-11 g/L, globulin 22-32 g/L, albumin:globulin ratio 0.27-0.37, creatinine 0-8 μmol/L, and osmolality 251.8-327.9 mOsm/kg. Reference intervals reported here can help veterinarians and fish health specialists monitor the health status of Striped catfish under recirculating aquaculture conditions for research, exhibition, and production purposes. © 2017 American Society for Veterinary Clinical Pathology.

  8. INFLUENCE OF FEEDING TIME ON FEED UTILIZATION BY SIAMESE CATFISH, Pangasius hypophthalmus JUVENILE

    Directory of Open Access Journals (Sweden)

    Nina Meilisza

    2010-06-01

    Full Text Available Siamese catfish (Pangasius hypophthalmus at juvenile stage is used as ornamental fish by fish hobbies because of their beautiful shape and movement. Feeding time is a part of feeding management to get the best and effective strategy on feed input. This study aimed to determine the growth of Siamese catfish juvenile by looking at the influence of feeding time on feed utilization. The average individual weight of juveniles used was ± 3.2 g stocked 15 fish per aquarium equipped with recirculation system. Two level factors in the factorial design consisted of 4 feeding times (at 8:00, at 12:00, at 16:00, and as control (8:00, 12:00, 16:00 and feeding level (45 g feed kg-1 fish, 90 g feed kg-1 fish. Interaction between feeding time and feeding level resulting eight types of treatments research, namely A (8/45, B (12/45, C (16/45, D (8:12:16/45, E (8:12:16/90, F (8/90, G (12/90, and H (16/90. The results showed that feeding time had significant effect on the specific growth rate, feed conversion, and protein efficiency ratio and were significantly different among the treatments (P<0.05. Besides feeding time control (treatments D and E, treatment C also showed the highest of specific growth rate (2.03%, protein efficiency ratio (1.46, and the lowest feed conversion ratio (1.85.

  9. Utilization of cinnamon Cinnamomum burmannii leaves and shrimp head in the feed on growth performance of catfish Pangasianodon hypopthalmus

    Directory of Open Access Journals (Sweden)

    Suclyadi Dairun

    2018-06-01

    Full Text Available ABSTRACTCatfish farming has confronted with the problem of slow growth to reach the fillet size and less tender of fish fillets. This study aimed to investigate the effect of cinnamon leaf flour and shrimp head flour in formulated diets on growth of catfish Pangasianodon hypopthalamus. Catfish with an initial weight of 207.04 ± 2.70 g/fish reared in 12 cages with a dimension of  2×1×1.5 m3 (L×W×H  and its fish density was 15 fishes for 60 days. Fish were fed with following experimental diets: (1 Control; (2 cinnamon leaf (1% (CL; (3 45% of protein source from shrimp head (SH; (4 CL+SH mix.  The fishes fed twice a day with feeding rate 3.5% of fish biomass. The study found that the use of cinnamon leaf and shrimp head increased the specific growth rate 1.67-1.70%, the feed efficiency 57.55-57.67%, and protein retention 55.61% compared to its control (P<0.05. Triglyceride level, cholesterol, and blood HDL were 416.00-524.05 mg/dL, 139.65-156.68 mg/dL, 73.18-103.70 mg/dL (P>0.05, respectively. HSI value ranged between 0.3-1.9% compared to its control (P<0.05.Keywords: Cinnamomum burmannii, feed, growth, Pangasianodon hypopthalmus, shrimp head.  ABSTRAKBudidaya ikan patin dihadapkan pada permasalahan pertumbuhan dan kualitas daging yaitu pertumbuhan yang lambat untuk mencapai ukuran fillet serta tekstur daging kurang kompak. Tujuan penelitian ini adalah untuk menguji pengaruh penggunaan tepung daun kayu manis dan tepung kepala udang dalam pakan terhadap pertumbuhan ikan patin Pangasianodon hypopthalmus. Ikan patin dengan bobot 207,04 ± 2,70 g/ekordipelihara selama 60 hari di hapa sebanyak 12 buah (berukuran 2×1×1,5 m3dengan padat tebar 15 ekor/jaring. Ikan diberi pakan perlakuan yang terdiri dari: (1 Kontrol; (2 daun kayu manis 1% (DKM; (3 45% sumber protein dari kepala udang (TKU; dan (4 campuran DKM+TKU. Pemberian pakan sebanyak 2 kali sehari dengan feeding rate 3,5% dari biomassa ikan. Hasil dari penelitian penggunaan daun kayu manis

  10. Effect of Linear Alkylbenzene Sulfonate on Mortality, Hatching Rate of Eggs and Abnormality of Catfish (Pangasius hypophthalmus Sauvage Larvae

    Directory of Open Access Journals (Sweden)

    E. Supriyono

    2007-01-01

    Full Text Available Linear alkylbenzene sulfonate (LAS surfactant in the water can affecting fish in all developmental stages.  This study was aimed to observe the effect of LAS on mortality, hatching rate of eggs, and abnormality of patin catfish (Pangasius hypophthalmus Sauvage larvae.   Fertilized eggs were incubated in water containing LAS at the dosages of 0.0, 0.5, 1.5, 3.0, 9.0, and 18.0 mg/L.  Eggs mortality was observed every 6 hours until larvae hatched (24 hours.  The results of study showed that the exposure of 18.0 mg LAS per liter water could put to death all the fertilized eggs and larvae be abnormal.  The exposure of LAS at concentration of 9.0 mg/L could kill 98% of eggs and hatching rate was only 2%.  The abnormality in larvae was bending in the body and tails. Keywords: patin catfish, Pangasius, Linear Alkylbenzene Sulfonate, LAS, abnormality   ABSTRAK Surfaktan Linear Alkylbenzene Sulfonate (LAS yang masuk ke dalam perairan sangat berpengaruh terhadap ikan dari stadia awal hidup ikan sampai dewasa. Penelitian ini bertujuan untuk mengetahui pengaruh LAS terhadap mortalitas, daya tetas telur dan abnormalitas larva ikan patin (Pangasius hypophthalmus Sauvage.  Telur ikan patin yang telah dibuahi di rendam dalam air yang mengandung LAS dengan konsentrasi 0,0; 0,5; 1,5; 3,0; 9,0 dan 18,0 mg/L.  Mortalitas telur dicatat setiap 6 jam sampai menetas (24 jam.  Hasil penelitian menunjukkan bahwa konsentrasi LAS sebesar 18,0 mg/L dapat mengakibatkan mortalitas telur dan abnormalitas pada larva secara total.  Konsentrasi LAS sebesar 9,0 mg/L dapat mematikan telur hingga 98% dan hanya menghasilkan daya tetas sebanyak 2%. Abnormalitas pada larva berupa pembengkokan pada tubuh dan ekor. Kata kunci: ikan patin, Pangasius, Linear Alkylbenzene Sulfonate, LAS dan abnormalitas

  11. Utilization of Cinnamon Leaf and Shrimp Flour as an Enhancer of Catfish Meat Quality

    Directory of Open Access Journals (Sweden)

    Mia Setiawati

    2017-04-01

    Full Text Available Catfish (Pangasianodon hypophthalmus is a freshwater fish that has been produced in the form of a filet. One of the problems in producing good catfish fillet is compactness and brightness of catfish farmed meat. This research aimed to get feed formulation as enhancer meat quality of striped catfish with added Cinnamon leaves flour (Cinnamomum burmannii and used shrimp head meal. A Fish with a weight of 208.98±25.76 g reared in 12 floating nets cage (2x1x1.5 m3 with density of 15 fish/nets for 60 days. As treatment, fish were fed with feed contains 1% cinnamon leaves, 45% shrimp head meal, and combined of cinnamon leaves and shrimp head meal, and as control used feed were formulated without cinnamon leaves and shrimp head meal. Fish were fed 2 times a daily with feeding rate 3.5% of average body weight of fish. The test parameters observed were physical, chemical and organoleptic test of catfish meat. The results showed feed with contains cinnamon leaves and shrimp head meal could decrease level of body fat 14.7% compared than control (p<0.05. Feed with used cinnamon leaves and shrimp head meal gave a texture of fillet fish more compact, elastic and color of fillet fish white. Keywords: Cinnamomum burmannii, fillet, shrimp head meal, feed formulated, Pangasianodon hypophthalmus

  12. Comparison of Lethal Concentrations (LC50-96 H of Cdcl2, Crcl3, and Pb (NO32 in Common Carp (Cyprinus carpio and Sutchi Catfish (Pangasius Hypophthalmus

    Directory of Open Access Journals (Sweden)

    Zeynab Abedi

    2012-11-01

    Full Text Available Background: The present study compared lethal concentrations (LC50-96 h of CdCl2, CrCl3, and Pb (NO32 between two scaled and scaleless freshwater fish species: Cyprinus carpio (Cyprinidae and Pangasius hypophthalmus (Pangasiidae. Methods: The experimental fishes were obtained from fish markets/ponds with average lengths and weights of approximately 11.68 ±1.92 and 9.8±1.9 cm, and 25.92±6.3 and 18.61±3.22 g for C. carpio and P. hypophthalmus, respectively. The fishes were exposed to different concentrations of cadmium (Cd (0, 10, 20, 40, 80, 100, 120, 200 mg L−1 lead (Pb (0, 20, 40, 50, 60, 90, 150 mg L−1, and chromium (Cr (0, 5, 10, 20, 30, 40 mg L−1 for 96 h. Physicochemical parameters, such as dissolved oxygen, pH, and temperature of aquaria as well as mortality rate of the fishes, were monitored daily. Results: The 50% lethal concentrations (LC50-96 h of CdCl2, CrCl3, and Pb (NO32 for P. hypophthalmus were found at 64.89, 7.46, and 48.06 mg L−1, and those of CdCl 2, CrCl 3, Pb (NO32 for C. carpio were detected at 84.8, 17.05, and 77.33 mg L−1. The ratios of heavy metal toxicity factors (TF were greater for common carp compared to those for the catfish. Conclusion: Our preliminary findings suggest that common carp C. carpio with higher LC50 (and LC100 values appears to be more tolerant to heavy metals exposure than the catfish (P. hypophthalmus. This may be due to the resistance to the heavy metals through protection from the carp's scaled body versus scaleless body of the catfish.

  13. Improvement of Nutritional Value of Instant Noodles by Fortifying with Catfish (Pangasius Hypophthalmus) Flour

    OpenAIRE

    Gusriadi, Depri; Sukmiwati, Mery; ', Dahlia

    2014-01-01

    This study was intended to evaluate the nutritional value of instant noodlesfortified with catfish flour. Instant noodles were prepared from a mixture of wheatflour (50%), sweat potato flour (10%), egg (15%), salt (0,7%), water (23%),cooking oil (0,3%) and Carboxy Methyl Cellulose (1%). The noodles wereprepared by fortifying the mixture with catfish flour at a level of 0%, 5%, 10%,and 15 % respectively. Instant noodles was evaluated for sensory quality andproximate composition (moisture, prot...

  14. THE EFFECT OF IMMERSION LENGTH IN PAPAYA LEAF SOLUTION (CARICA PAPAYA L. TOWARD FERTILIZATION AND HATCHING OF IRIDESCENT SHARKS (PANGASIANODON HYPOPHTHALMUS S.

    Directory of Open Access Journals (Sweden)

    Eka S.H.

    2018-02-01

    Full Text Available Iridescent sharks is one kind of freshwater fish that is quite economical. However, the availability of the fish eggs is rather problematic due to the hatcheries, i.e. the adhesive nature of the eggs. Papaya leaves contain papain enzymes that can reduce the adhesive nature of eggs. The purpose of this study was to determine the effect of papaya leaf solution with different immersion period on the successful hatching of the iridescent sharks (P. hypophthalmus. This study was conducted experimentally using a complete randomized design. There were five treatments, i.e. (K 0 seconds, (A 30 seconds, (B 60 seconds, (C 90 seconds, (D 120 seconds, and (E 150 seconds with three-time repetition. The result of this study shows that the best hatching (80.31% happens to the eggs immersed in papaya leaf solution for 60 seconds (treatment B.

  15. Effects of nitrite exposure on functional haemoglobin levels, bimodal respiration, and swimming performance in the facultative air-breathing fish Pangasianodon hypophthalmus

    Energy Technology Data Exchange (ETDEWEB)

    Lefevre, Sjannie, E-mail: sjannie.lefevre@biology.au.dk [Zoophysiology, Department of Biological Sciences, Aarhus University, Aarhus (Denmark); Jensen, Frank B. [Department of Biology, University of Southern Denmark, Odense (Denmark); Huong, Do.T.T. [College of Aquaculture and Fisheries, Can Tho University, Can Tho City (Viet Nam); Wang, Tobias [Zoophysiology, Department of Biological Sciences, Aarhus University, Aarhus (Denmark); Phuong, Nguyen T. [College of Aquaculture and Fisheries, Can Tho University, Can Tho City (Viet Nam); Bayley, Mark [Zoophysiology, Department of Biological Sciences, Aarhus University, Aarhus (Denmark)

    2011-07-15

    In this study we investigated nitrite (NO{sub 2}{sup -}) effects in striped catfish, a facultative air-breather. Fish were exposed to 0, 0.4, and 0.9 mM nitrite for 0, 1, 2, 4, and 7 days, and levels of functional haemoglobin, methaemoglobin (metHb) and nitrosyl haemoglobin (HbNO) were assessed using spectral deconvolution. Plasma concentrations of nitrite, nitrate, chloride, potassium, and sodium were also measured. Partitioning of oxygen consumption was determined to reveal whether elevated metHb (causing functional hypoxia) induced air-breathing. The effects of nitrite on maximum oxygen uptake (MO{sub 2max}) and critical swimming speed (U{sub crit}) were also assessed. Striped catfish was highly tolerant to nitrite exposure, as reflected by a 96 h LC{sub 50} of 1.65 mM and a moderate nitrite uptake into the blood. Plasma levels of nitrite reached a maximum after 1 day of exposure, and then decreased, never exceeding ambient levels. MetHb, HbNO and nitrate (a nitrite detoxification product) also peaked after 1 day and then decreased. Only high levels of nitrite and metHb caused reductions in MO{sub 2max} and U{sub crit}. The response of striped catfish contrasts with that seen in most other fish species and discloses efficient mechanisms of combating nitrite threats. Furthermore, even though striped catfish is an efficient air-breather, this species has the ability to sustain aerobic scope and swimming performance without air-breathing, even when faced with nitrite-induced reductions in blood oxygen carrying capacity. Our study is the first to confirm that high levels of nitrite and metHb reduce MO{sub 2max} and thereby aerobic scope, while more moderate elevations fail to do so. Further studies are needed to elucidate the mechanisms underlying the low nitrite accumulation in striped catfish.

  16. Effects of nitrite exposure on functional haemoglobin levels, bimodal respiration, and swimming performance in the facultative air-breathing fish Pangasianodon hypophthalmus.

    Science.gov (United States)

    Lefevre, Sjannie; Jensen, Frank B; Huong, Do T T; Wang, Tobias; Phuong, Nguyen T; Bayley, Mark

    2011-07-01

    In this study we investigated nitrite (NO₂⁻) effects in striped catfish, a facultative air-breather. Fish were exposed to 0, 0.4, and 0.9 mM nitrite for 0, 1, 2, 4, and 7 days, and levels of functional haemoglobin, methaemoglobin (metHb) and nitrosyl haemoglobin (HbNO) were assessed using spectral deconvolution. Plasma concentrations of nitrite, nitrate, chloride, potassium, and sodium were also measured. Partitioning of oxygen consumption was determined to reveal whether elevated metHb (causing functional hypoxia) induced air-breathing. The effects of nitrite on maximum oxygen uptake (MO(2max)) and critical swimming speed (U(crit)) were also assessed. Striped catfish was highly tolerant to nitrite exposure, as reflected by a 96 h LC₅₀ of 1.65 mM and a moderate nitrite uptake into the blood. Plasma levels of nitrite reached a maximum after 1 day of exposure, and then decreased, never exceeding ambient levels. MetHb, HbNO and nitrate (a nitrite detoxification product) also peaked after 1 day and then decreased. Only high levels of nitrite and metHb caused reductions in MO(2max) and U(crit). The response of striped catfish contrasts with that seen in most other fish species and discloses efficient mechanisms of combating nitrite threats. Furthermore, even though striped catfish is an efficient air-breather, this species has the ability to sustain aerobic scope and swimming performance without air-breathing, even when faced with nitrite-induced reductions in blood oxygen carrying capacity. Our study is the first to confirm that high levels of nitrite and metHb reduce MO(2max) and thereby aerobic scope, while more moderate elevations fail to do so. Further studies are needed to elucidate the mechanisms underlying the low nitrite accumulation in striped catfish. Copyright © 2011 Elsevier B.V. All rights reserved.

  17. Effects of nitrite exposure on functional haemoglobin levels, bimodal respiration, and swimming performance in the facultative air-breathing fish Pangasianodon hypophthalmus

    International Nuclear Information System (INIS)

    Lefevre, Sjannie; Jensen, Frank B.; Huong, Do.T.T.; Wang, Tobias; Phuong, Nguyen T.; Bayley, Mark

    2011-01-01

    In this study we investigated nitrite (NO 2 - ) effects in striped catfish, a facultative air-breather. Fish were exposed to 0, 0.4, and 0.9 mM nitrite for 0, 1, 2, 4, and 7 days, and levels of functional haemoglobin, methaemoglobin (metHb) and nitrosyl haemoglobin (HbNO) were assessed using spectral deconvolution. Plasma concentrations of nitrite, nitrate, chloride, potassium, and sodium were also measured. Partitioning of oxygen consumption was determined to reveal whether elevated metHb (causing functional hypoxia) induced air-breathing. The effects of nitrite on maximum oxygen uptake (MO 2max ) and critical swimming speed (U crit ) were also assessed. Striped catfish was highly tolerant to nitrite exposure, as reflected by a 96 h LC 50 of 1.65 mM and a moderate nitrite uptake into the blood. Plasma levels of nitrite reached a maximum after 1 day of exposure, and then decreased, never exceeding ambient levels. MetHb, HbNO and nitrate (a nitrite detoxification product) also peaked after 1 day and then decreased. Only high levels of nitrite and metHb caused reductions in MO 2max and U crit . The response of striped catfish contrasts with that seen in most other fish species and discloses efficient mechanisms of combating nitrite threats. Furthermore, even though striped catfish is an efficient air-breather, this species has the ability to sustain aerobic scope and swimming performance without air-breathing, even when faced with nitrite-induced reductions in blood oxygen carrying capacity. Our study is the first to confirm that high levels of nitrite and metHb reduce MO 2max and thereby aerobic scope, while more moderate elevations fail to do so. Further studies are needed to elucidate the mechanisms underlying the low nitrite accumulation in striped catfish.

  18. Safety dose of three commercially used growth promoters: nuricell- aqua, hepaprotect-aqua and rapid-grow on growth and survival of Thai pangas (Pangasianodon hypophthalmus

    Directory of Open Access Journals (Sweden)

    Md. Ariful Islam

    2014-02-01

    Full Text Available Objective: To optimize the dose of 3 commonly used growth promoters, viz., Nuricell-Aqua (composition: glucomannan complex and mannose polymer, Hepaprotect-Aqua (composition: β-glucan, mannose polymer and essential oil and Rapid-Grow (composition: organic acid and their salt, β-glucan, mannose oligosaccharide and essential oil, using Thai pangas (Pangasiandon hypophthalmus as cultured species. Methods: Thai pangas fingerlings with an average length and weight of 11 cm and 10 g were reared under laboratory condition and growth promoters were fed after incorporating them with a test diet at a ratio of 10% of their body weight for a period of 28 d. Estimation of data on growth such as weight gain (g, specific growth rate, survivability (% test in each aquarium were conducted and data were analyzed using statistical software. Results: After 28 d of feeding with Nutricell-Aqua, 10 mg/(20 g feed·day, which was the dose recommended by the manufacturer, was found better. When Hepaprotect-Aqua and Rapid-Grow were employed, performance was found to be better with the dose of 60 mg/(20 g feed·day which was 1.5 times higher than the dose recommended by the corresponding manufacturer. Conclusions: These results suggest that chemicals and feed additives marketed in Bangladesh Fish Feed Market need further testing under Bangladesh climatic condition before being marketed.

  19. Effect of LH-RHa Injection trough W/O/W LG (C-14 Emulsion on Gonad Development of Catfish (Pangasious hypophthalmus

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    N. Potalangi

    2007-12-01

    Full Text Available The objective of this research was to study the effect of LHRHa gonad maturity in broodstock of P. hypophthalmus through W/O/W LG (C-14 emulsion injection. The treatments consisted of control (A, 50 µg/kg fish wight (B, and 100 µg/kg fish weight (C, with five replications of each. Fish weight at the beginning of experiment was 2.0 kg/individual. The result showed that LHRHa in W/O/W emulsion had positive effect on egg maturation. This was shown by the value of average of eggs diameter. The maximum size of egg diameter for fish 701.52 ± 17.56 µm. The size of eggs was more homogenous in group B than those of group C and A. it was concluded that injection of LHRHa in W/O/W LG (C-14 emulsion if effective in promoting gonad maturation and oocyte development in the catfish

  20. Amino Acid Profile and Volatile Flavour Compounds of Raw and Steamed Patin Catfish (Pangasius hypophthalmus) and Narrow-barred Spanish Mackerel (Scomberomorus commerson)

    Science.gov (United States)

    Pratama, Rusky I.; Rostini, I.; Rochima, E.

    2018-02-01

    Fish species and processing methods could affect the volatile flavour composition and amino acid profile of fishery commodity. The objectives of this study were to identify volatile components and amino acid profile of two considered predominant fish species in Indonesia which are freshwater Patin catfish (Pangasius hypophthalmus) and marine water fish, Spanish mackerel (Scomberomorus commerson). The methods used in this study were to detect volatile compounds using Gas Chromatography/Mass Spectrometry (GC/MS) on fresh and steamed of both species samples (100°C for 30 minutes) and amino acid profile were also analyzed using High Performance Liquid Chromatography (HPLC). The volatile components analysis successfully detects as much as 29 and 59 volatiles compounds in fresh and steamed Patin catfish respectively, while 37 and 102 compounds were detected in fresh and steamed Spanish mackerel samples. Most of detected components derives from hydrocarbons, aldehydes, alcohols and ketone groups which could affected by their chemical composition and resulted from various thermal involved reaction. The amino acids profile identification results showed that glutamic acid was found higher compared to other amino acids standards in both samples. Glutamic acid is non-essential amino acid which is important in umami taste substances.

  1. THE EFFECTIVITY TEST OF SHEEP RUMEN LIQUOR ENZYME ADDED TO PALM KERNEL MEAL ON ITS DECREASE OF CRUDE FIBER AND APPARENT DIGESTIBILITY COEFFICIENT FOR CATFISH Pangasius hypophthalmus DIET

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    Wahyu Pamungkas

    2011-12-01

    Full Text Available Two experiments were conducted to evaluate the hydrolysis of fiber content in palm kernel meal (PKM by sheep rumen liquor enzyme and to know the apparent digestibility coefficient of hydrolyzed PKM for catfish Pangasius hypophthalmus. The first trial examined effectivity of sheep rumen liquor enzyme to decrease crude fiber content of PKM. The added volume of sheep rumen liquor enzyme was 0, 20, 40, 60, 80, and 100 mL/kg PKM and then it was incubated for 0, 12, and 24 hours. A factorial completely randomized experimental design consisted of 2 variables and triplicates were selected. The second trial was conducted to evaluate the apparent digestibility coefficients of hydrolized PKM for catfish. Apparent digestibility coefficients were determined using chromic oxide indicator added to both reference and test diets. The feed ingredients used in the trial were hydrolyzed PKM (PKMe and unhydrolyzed PKM (PKM. Ten fishes with weighing around 20 g were used in the trial and held in 80 l tanks. Feces were collected from three replicate groups of fish using a fecal collection column attached to fish rearing tank. PKM hydrolyzed with 100 mL/kg and incubated for 24 hour showed the lowest crude fiber content (6.99% among the treatments (P<0.05. Apparent digestibility coefficient of hydrolyzed PKM was 57.57% compared with unhydrolyzed PKM 15.31%. Based on the evaluation in those parameters it was concluded that sheep rumen liquor enzyme added to PKM was effective to decrease crude fiber content of PKM and improve apparent digestibility coefficient of PKM for catfish.

  2. Utilization of Cinnamon Leaf and Shrimp Flour as an Enhancer of Catfish Meat Quality

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    Mia Setiawati

    2017-05-01

    Full Text Available Catfish (Pangasianodon hypophthalmus is a freshwater fish that has been produced in the form of a filet. One of the problems in producing good catfish fillet is compactness and brightness of catfish farmed meat. This research aimed to get feed formulation as enhancer  meat quality of striped catfish with added Cinnamon leaves flour (Cinnamomum burmannii  and used shrimp head meal. A Fish with a weight of  208.98±25.76 g reared in 12 floating nets cage (2x1x1.5 m3 with density of 15 fish/nets for 60 days. As treatment, fish were fed with feed contains 1% cinnamon leaves,  45% shrimp head meal, and combined of cinnamon leaves and shrimp head meal, and as control used feed were formulated without cinnamon leaves and shrimp head meal. Fish were fed 2 times a daily with feeding rate 3.5% of average body weight offish. The test parameters observed were physical, chemical and organoleptic test of catfish meat. The results showed feed with contains cinnamon leaves and shrimp head meal could decrease level of body fat 14.7% compared than control (p<0.05. Feed with used cinnamon leaves and shrimp head meal gave a texture offillet fish more compact,  elastic and color of fillet fish white.

  3. An evaluation of fish health-management practices and occupational health hazards associated with Pangasius catfish (Pangasianodon hypophthalmus) aquaculture in the Mekong Delta, Vietnam

    DEFF Research Database (Denmark)

    Phu, TranMinh; Nguyen Thanh, Phuong; Dung, Tu Thanh

    2016-01-01

    released antimicrobials and resistance development. Small-scale farmers were at particular occupational health risks when handling antimicrobials and other chemicals, e.g. mixing medicated feed with bare hands. There is an urgent need to improve knowledge and use innovative approaches, e.g. private......-public partnerships, to assure a prudent use of chemicals, to improve capacity and access to disease diagnosis, particularly for small-scale grow-out farmers and nurseries. Efforts to control use of antimicrobials in aquaculture should be coordinated with the livestock and human health sectors taking an One-Health...

  4. Two-year intervention trial to control of fish-borne zoonotic trematodes in giant gourami (Osphronemus goramy) and striped catfish (Pangasianodon hypophthalmus) in nursery ponds in the Mekong Delta, Vietnam

    DEFF Research Database (Denmark)

    Madsen, Henry; Thien, P. C.; Nga, H. T. N.

    2015-01-01

    Fish-borne zoonotic trematode parasites (FZT) pose a food safety and public health problem in Vietnam. The transmission cycle is complex as domestic animals, especially dogs, cats, fish-eating birds and pigs together with humans serve as reservoir hosts and contribute to FZT egg contamination of ...

  5. Kajian patogenisitas bakteri Edwardsiella ictaluri pada ikan patin Pangasionodon hypophthalmus

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    Wiwik Susanti

    2016-12-01

    Full Text Available ABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC, bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen and internal organs (liver, kidney, and brain were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05 compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus.  Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC, pathogenicity, striped catfish  ABSTRAK Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini

  6. GFP tagged Vibrio parahaemolyticus Dahv2 infection and the protective effects of the probiotic Bacillus licheniformis Dahb1 on the growth, immune and antioxidant responses in Pangasius hypophthalmus.

    Science.gov (United States)

    Gobi, Narayanan; Malaikozhundan, Balasubramanian; Sekar, Vijayakumar; Shanthi, Sathappan; Vaseeharan, Baskaralingam; Jayakumar, Rengarajan; Khudus Nazar, Abdul

    2016-05-01

    In this study, the pathogenicity of GFP tagged Vibrio parahaemolyticus Dahv2 and the protective effect of the probiotic strain, Bacillus licheniformis Dahb1 was studied on the Asian catfish, Pangasius hypophthalmus. The experiment was carried out for 24 days with three groups and one group served as the control (without treatment). In the first group, P. hypophthalmus was orally infected with 1 mL of GFP tagged V. parahaemolyticus Dahv2 at two different doses (10(5) and 10(7) cfu mL(-1)). In the second group, P. hypophthalmus was orally administrated with 1 ml of the probiotic B. licheniformis Dahb1 at two different doses (10(5) and 10(7) cfu mL(-1)). In the third group, P. hypophthalmus was orally infected first with 1 mL of GFP tagged V. parahaemolyticus Dahv2 followed by the administration of 1 mL of B. licheniformis Dahb1 (combined treatment) at two different doses (10(5) and 10(7) cfu mL(-1)). The growth, immune (myeloperoxidase, respiratory burst, natural complement haemolytic and lysozyme activity) and antioxidant (glutathione-S-transferase, reduced glutathione and total glutathione) responses of P. hypophthalmus were reduced after post infection of GFP tagged V. parahaemolyticus Dahv2 compared to control. However, after administration with the probiotic B. licheniformis Dahb1 at 10(5) cfu mL(-1), P. hypophthalmus showed significant increase in the growth, immune and antioxidant responses compared to 10(7) cfu mL(-1). On the otherhand, the growth, immune and antioxidant responses of P. hypophthalmus infected and administrated with combined GFP tagged Vibrio + Bacillus at 10(5) cfu mL(-1) were relatively higher than that of GFP tagged V. parahaemolyticus Dahv2 and control groups but lower than that of probiotic B. licheniformis Dahb1 groups. The results of the present study conclude that the probiotic B. licheniformis Dahb1 at 10(5) cfu mL(-1) has the potential to protect the P. hypophthalmus against V. parahaemolyticus Dahv2 infection by enhancing the growth

  7. PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp.

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    Irzal Effendi

    2003-05-01

    Full Text Available Development of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae.  The 1 day old larvae (day after hatching, with 3,37-3,97 mm length and 0,62-0,79 mg weight, were reared in aquarium 60x50x40 cm with stocking  density of 20 fish/l.  Larvae were fed  Artemia dan tubificid worms 2-8 dan 7-15 days after hatching (dAH,  respectively (schedule I;  2-6 and  5-15 dAH (schedule II; and 2-4 and 5-15 dAH (schedule III.  Chlorella was ready to eat by larvae at the entirely rearing.  For enzyme assay, larvae were sampled from each aquarium at stages of 1, 2, 3, 5, 7, 10 and 15 dAH.    Protease and lipase activity were detected in digestive tract of  1 dAH larvae.   Digestive enzymes development have a similar pattern in larvae for all feeding schedules.  Protease activity  decreased with the increasing of age until 3 dAH, then increased  until the larvae reached 7 dAH, and sharply decreased until 10 dAH and then slowly decreased thereafter. Lipase activity tended to increase slowly with age up to 3 dAH, and increased sharply until 5 dAH, and then decreased sharply until 7 dAH  before decreased again up to the end of rearing.  Amylase activity in larvae increased slowly with the increasing of age up to 5 dAH, then increased sharply until 7 dAH, and decreased thereafter.  In dimly lighted larvae, amylase activity decreased before increased up to 12 d AH, then decreased thereafter.  The amount of food organisms in larval gut, body weight and length, and survival rate of larvae were also measured and discussed.Key Words:  Digestive enzymes, development, larvae, patin catfish, Pangasius hypophthalmus ABSTRAKPenelitian ini bertujuan untuk mengetahui perkembangan enzim protease, lipase dan amilase saluran pencernaan larva ikan patin akibat perubahan skedul pemberian pakan.  Larva ikan patin (panjang 3,77–3,97 mm dan bobot 0,62-0,79 mg berumur 1 hari dipelihara di akuarium 60x

  8. Effect of chitosan on shelf life of restructured fish products from pangasius (pangasianodon hypophthalmus) surimi during chilled storage.

    Science.gov (United States)

    Jeyakumari A; George Ninan; Joshy C G; Parvathy U; Zynudheen A A; Lalitha K V

    2016-04-01

    In the present study, restructured products were prepared from pangasius surimi and their qualities were analysed under chilled storage. Pangasius surimi had 75.82 % moisture, 16.91 % protein, 2.76 % fat and 0.95 % ash. Restructured products were prepared in three different formulations by incorporating corn starch (10 %) and chitosan (0.75 %). Formulation containing only corn starch (10 %) was served as control. In all the formulations, mono unsaturated fatty acids were higher (45.14 %). The total volatile base nitrogen (TVB-N) showed an increasing trend and it was found to be higher in control (4.8 mg/100 g) on 10(th) day than the chitosan incorporated sample (3.5-4.2 mg/100 g) on 17(th) day during chill storage. Similarly, peroxide value (PV) was found to higher (8.85 milliequivalent of O2/kg) in control than the chitosan incorporated sample (4.5-6.8 milliequivalent of O2/kg) on 10(th) day. All the three formulations had an acceptable level of thiobarbituric acid (TBA) value that ranged between 0.023-0.098 mg of malanoldehyde/kg during chilled storage. Based on the sensory and microbiological analysis, products prepared without chitosan had a shelf life of 10 day whereas, products incorporated with chitosan had an extended shelf life of 17 day.

  9. GONAD REMATURATION ON Pangasionodon hypophthalmus FEMALE THROUGH INJECTION OF PREGNANT MARE SERUM GONADOTROPIN AND HUMAN CHORIONIC GONADOTROPIN

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    Evi Tahapari

    2014-06-01

    Full Text Available The success of spawning is influenced by internal and external factors. One of the factors that affect the var iabi li ty of Pangasianodon hypophthalmus female reproductive is the change of seasons that cause disrupted continuity of the seed availability, especially in the dry season. In the present study, combination of PMSG (pregnant mare serum gonadotropin + HCG (hormone chorionic gonadotropin hormone injections was done to induce gonad development. The treatments in this study were without hormone injections as control (A, injection of PMSG 10 IU/kg + HCG 10 IU/kg (B, and injection of PMSG 20 IU/kg HCG + 10 IU/kg (C. Injections were conducted at intervals of two weeks as many as six times. The results showed that gonad maturation generally occurs 2-4 weeks after estradiol-17 peak. PMSG + HCG hormone injections gave a significant effect on increasing the quantity and quality of eggs production. The fecundity in the A, B, C treatments, were 233,700±220,676; 300,305±24,581 and 488,433±142,228; respectively. Number of larvae produced from the A, B, C treatments, were 156,979±170,838; 229,997±18,081 and 362,713±101,850; respectively. Combination of PMSG 20 IU/kg + HCG 10 IU/kg hormone injection gave the best result on fecundity and the number of larvae production.

  10. HISTOPATOLOGI INSANG IKAN PATIN SIAM (Pangasius hypophthalmus YANG TERINFESTASI TREMATODA MONOGENEA

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    Ida Ayu N. S. Utami

    2017-08-01

    Full Text Available Ikan patin siam (Pangasius hypophthalmus yang terinfestasi parasit trematoda monogenea tidak mudah dikenali gejala klinisnya secara spesifik, mengingat parasit ini menyerang organ insang. Infeksi parasit ini dapat menyebabkan kematian ikan sehingga sangat merugikan budidaya ikan patin siam. Tujuan penelitian adalah memberikan informasi secara histopatologi tentang perubahan jaringan insang ikan patin siam yang terinfestasi parasit trematoda monogenea. Penelitian ini diawali dengan pemeriksaan natif insang patin siam yang terinfestasi parasit trematoda monogenea dan dilanjutkan dengan pemeriksaan histopatologi. Pengambilan sampel ikan patin siam dilakukan sebanyak dua kali pada Mei dan Oktober 2015 di beberapa kolam budidaya. Pengamatan sampel dilakukan secara mikroskopik di Laboratorium Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan, Palembang. Hasil penelitian menunjukkan bahwa dari total 150 sampel yang diamati terdapat 35 sampel atau kasus insang ikan yang terinfestasi parasit trematoda monogenea yaitu: masing-masing sebanyak 10 sampel diperoleh pada Mei dan 25 sampel pada Oktober. Pemeriksaan patologi terhadap organ insang yang terinfestasi trematoda monogenea menunjukkan bahwa lamella insang mengalami pembengkakan dan berwarna merah pucat. Parasit trematoda monogenea pada insang atau yang lebih dikenal dengan cacing insang memiliki panjang tubuh berkisar antara 0,7-0,9 mm dengan lebar 0,05-0,10 mm. Pengamatan histopatologi menunjukkan bahwa jaringan insang yang terinfestasi parasit trematoda monogenea ditandai adanya perubahan yang konsisten, yaitu hiperplasia tulang rawan hyalin, proliferasi sel mukus, hiperplasia lamella sekunder, dan fusi lamella sekunder. Perubahan ini dapat mengakibatkan kematian pada ikan akibat kekurangan oksigen dan perubahan osmoregulasi ion dalam tubuh ikan. The gills of striped catfish (Pangasius hypophthalmus infested with parasitic trematodes monogenea are not easy to observe specifically

  11. Catfish Biology and Farming.

    Science.gov (United States)

    Dunham, Rex A; Elaswad, Ahmed

    2018-02-15

    This article summarizes the biology and culture of ictalurid catfish, an important commercial, aquaculture, and sport fish family in the United States. The history of the propagation as well as spawning of common catfish species in this family is reviewed, with special emphasis on channel catfish and its hybridization with blue catfish. The importance of the channel catfish female×blue catfish male hybrid, including current and future methods of hybrid catfish production, and the potential role it plays in the recovery of the US catfish industry are discussed. Recent advances in catfish culture elements, including environment, management, nutrition, feeding, disease control, culture systems, genetic improvement programs, transgenics, and the application of genome-based approaches in catfish production and welfare, are reviewed. The current status, needs, and future projections are discussed, as well as genetically modified organism developments that are changing the future.

  12. Copper use and accumulation in catfish culture in the Mekong Delta, Vietnam.

    Science.gov (United States)

    Marcussen, Helle; Løjmand, Helle; Dalsgaard, Anders; Hai, Dao M; Holm, Peter E

    2014-01-01

    Aquaculture of Pangasius hypophthalmus (striped catfish) in Vietnam reached 1.1 million tonnes in 2011 and catfish fillets are exported worldwide. The intensive cultures of catfish mainly in earth ponds have made it necessary to apply CuSO4 and other chemicals to control external parasites and other pathogens. However, accumulation of Cu in aquaculture ponds may pose a hazard to growth of fish or to the aquatic environment. The aim of this study was to determine accumulation of Cu in sediment, water and fish in a catfish pond with a history of repeated treatment with CuSO4 in the Mekong Delta, Vietnam. Copper concentrations in pond sediment were in the interval 21.3-45.7 mg kg(-1) dw and did not exceed the Vietnamese values for soil to be used for agricultural production (70 mg kg(-1) dw.). During three samplings the total mean concentration of Cu in pond water (4 μg L(-1)) did not exceed the LC50-value (70 μg L(-1)) for catfish and the mean dissolved concentration of Cu (0.986 μg L(-1)) did not seem to constitute a risk for the stability of the aquatic ecosystem. No significant variation in Cu concentrations between sampling sites in the pond and depth of sediment profile were determined. The accumulation of Cu in catfish was highest in the liver compared to the skin, gills and muscle tissue. With the current practice of removing pond sludge three to four times during a production cycle little if any Cu seems to accumulate in catfish ponds despite repeated anti-parasite treatments with CuSO4. Further studies are needed to assess the eco-toxicity and impact on agricultural production when pond sediment is discharged into aquatic recipients and used as soil fertilizer.

  13. KINERJA REPRODUKSI DENGAN INDUKSI OODEV DALAM VITELOGNESIS PADA REMATURASI INDUK IKAN PATIN (Pangasius hypophthalmus DI DALAM WADAH BUDIDAYA

    Directory of Open Access Journals (Sweden)

    Agustinus Agustinus

    2016-06-01

    Full Text Available The objective of this research was to study the effect of Oodev as know as PMSGad gonad rematurity in infertile broodstock of P. hypophthalmus by Oodev Hormone injection. The treatments consisted of control 0 ml Oodev/kg fish weight (D, 0,3 ml Oodev/kg fish weight (A. 0,5 ml Oodev/kg fish weight (B and 0,ml Oodev/kg fish weight (C. withfourreplications of each. Fish weight at the beginning of experiment was 5 to 7 kg/individual. The result showed that Oodev had positive effect on egg maturation. This was shown by the value of average of eggs diameter. The maximum size of egg diameter for fish with hormone injection were larger than without injection. The best treatment was 0,5 ml Oodev/kg fish weight(B and the largest diameter was 701.52 ± 17.56 μrn. The size of eggs was more homogenous in group B than those of group C and A. It was concluded that injection of Oodev (PMSGad is effective in promoting gonad maturation and oocyte development in the catfish.

  14. Potential contribution of fish feed and phytoplankton to the content of volatile terpenes in cultured Pangasius (Pangasianodon hypophthalmus) and Tilapia (Oreochromis niloticus)

    DEFF Research Database (Denmark)

    Podduturi, Raju; Petersen, Mikael Agerlin; Mahmud, Sultan

    2017-01-01

    Geosmin and 2-methylisoborneol are the most recognized off-flavors in freshwater fish, but terpenes may also contribute off-flavor in fish. We identified six monoterpenes, 11 sesquiterpenes, and three terpene-related compounds in pangasius and tilapia from aquaculture farms in Bangladesh. The con...

  15. Species-specific content of As, Pb, and other elements in pangas (Pangasianodon hypophthalmus) and tilapia (Oreochromis niloticus) from aquaculture ponds in southern Bangladesh

    DEFF Research Database (Denmark)

    Marcussen, Helle; Alam, Md. Ariful; Rahman, Md. Mizanur

    2014-01-01

    Content of arsenic and lead and 10 other potentially toxic elements (Ba, Ce, Cr, Cu, Fe,Mg,Mn, Se, Sr and Zn) was determined in tilapia and pangas from aquaculture ponds in southern Bangladesh. Tilapia had 1.6- to 4.3-fold higher content of Mn, As, Sr, and Ba than pangas, possibly caused...... by different feeding habits of the two fish. The other elements had similar concentrations in both species. Content of As in tilapia and pangas was 0.37 and 0.11 μg g−1, respectively, while Pb made up 0.056 and 0.051 μg g−1, respectively. Water treatment during the farming period (sand filtration and probiotic...... bacteria) and final depuration in groundwater for up to 48 h had no effect on content of the elements. For As, consumption of 100 g fresh fish per day contained 1.3% (pangas) and 5% (tilapia) of the maximum tolerable daily intake according to FAO recommendations. Relative to whole tilapia froma lake near...

  16. Comparison of cadmium and chromium bioconcentration factors between scaled and scale less fish species: common carp and sutchi (striped catfish

    Directory of Open Access Journals (Sweden)

    Sideh Zainab Abedi

    2014-10-01

    Full Text Available Background: Water-borne, indissoluble heavy metals are bioaccumulated in fish (human food source. In this research, chromium (Cr and cadmium (Cd bioconcentration factors (BCFs in the skin, gills, scales, livers and muscles of two widely-consumed fish species, scaled common carp Cyprinus carpio and scaleless catfish Pangasius hypophthalmus were compared. Material and Methods: Lethal concentrations of Cd and Cr (64.89 & 7.46, and 84.8 & 17.05 mg/L for the catfish and carp, respectively were determined during 96 hrs (96 h LC50, and the fishes were exposed for 15 days. Then the tissue samples were chemically digested and the contents of Cd and Cr were determined using atomic absorption. Results: Total contents of Cr and Cd in the catfish's tissues were 2286.11 & 360.73, and those of Cr & Cd in carp were 734.71 & 725.67 μg/g.dw (excluding the scales, respectively. Metal concentrations in the water residues (day 15 revealed lower Cr and Cd (0.059 & 0.0036 mg/L in the catfish than those in the carp media (0.1 & 0.0412 mg/L, respectively. In common carp, BCF of Cd was as liver > skin > gills > muscle > scales, and those in the catfish marked as liver>muscle >gills >skin. The BCF of Cr in common carp ranked as gills>liver>skin>scales>muscle, and the catfish showing a BCF pattern of Cr as liver> muscle>skin>gills. Conclusion: This study signifies that small amounts of cadmium and chromium with high BCFs especially in the muscles of scale less fish (catfish threat consumers’ health.

  17. and Asian catfish, Clarias batrachus

    African Journals Online (AJOL)

    ALEX

    2013-02-27

    Feb 27, 2013 ... crossing Indian catfish, Heteropneustes fossilis (Bloch) and Asian catfish, Clarias batrachus (Linn.) by ... Extensive culture of H. fossilis is prevalent in India, but with less growth, when ..... Kligerman AD, Bloom SE (1977).

  18. Ekstraksi dan karakterisasi minyak dari kulit ikan patin (Pangasius hypophthalmus

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    Nurjanah .

    2014-01-01

    Full Text Available Catfish is playing an important role in aquaculture production; it is one of the primary commodities of inlandfisheries industry. Catfish meat has a yield of 49% and resulted in 51% of waste products. Thus catfish waste product has potency as source of fish oil which riches of omega-3. The Purpose of this study was to evaluate the suitable extaction method to obtain the high quality of fish oil from catfish skin. Based on the oxidative quality analysis data obtained extraction temperature of 60°C gives the best quality of fish oil with parameters such as peroxide value, free fatty acid, p-anisidine value, acid value and total oxidation of 38 meq/kg, 0.45%, 107,5 meq/kg, 895 mg KOH/kg and 187.5, respectively. The best yield was 18.75% which was obtained at extraction temperature of 75 °C. It was concluded that best extraction at 60°C, for 30 minutes, with solvent and sample ratio 1:1.

  19. Cytochrome oxidase-I sequence based studies of commercially available Pangasius hypophthalmus in Italy

    Directory of Open Access Journals (Sweden)

    Federica Bellagamba

    2015-09-01

    Full Text Available Pangasius hypophthalmus is one of the fish consumed in the Italian diet. It is farmed and imported from Mekong delta region of Vietnam. Among several types of Pangasius, Tra (Pangasius hypophthalmus is permitted for sales by the European Union. Since these fish species are often allegedly substituted with other morphologically similar fish due to commercial benefits, authentication of the products in the international markets become often necessary to prevent fraud and safety issues. In addition, this fish is imported as fillets without skin and bone, thus leaving the consumer’s at the risk of buying a substandard nutritional food. In this article we present the molecular approach we developed to identify Pangasius hypophthalmus from other closely related species based on cytochrome oxidase-I (COI mitochondrial barcoding gene and further described the variants in the studied population genetic of this species. Fifty-one samples of Pangasius hypophthalmus fillets labelled as Pangasio were obtained from various markets around Milan and their COI mitochondrial barcoding gene was sequenced and studied in our bioinformatics pipeline. All samples were successfully amplified and Basic Local Alignment Search Tool results of the amplified region confirmed that all sequences analysed belonged to Pangasius hypophthalmus. Based on the variations in their barcoding region single nucleotide polymorphisms were identified and delineative statistics was calculated on the sequences. Although Pangasius hypophthalmus is considered as a monophyly, seven polymorphisms were identified. The neighbour-joining tree and the Median-joining network of haplotypes showed for all the identified haplotypes a unique cluster, with the exception of one sample.

  20. Production and characterization of monoclonal antibodies specific to pangasius catfish, basa, and tra.

    Science.gov (United States)

    Gajewski, K G; Chen, Y-T; Hsieh, Y-H P

    2009-04-01

    Four IgG (subclass IgG1) class monoclonal antibodies (MAbs) strongly reactive to Asian farm-raised Pangasius catfish, tra (Pangasius hypophthalmus) and basa (Pangasius bocourti), have been developed. These MAbs were raised by immunizing an animal with thermal-stable crude sarcoplasmic protein extract of cooked tra. The MAbs were selected by screening hybridoma clones against more than 70 common fish and meat protein extracts. Two MAbs, T7E10 and T1G11, were found to be specific to the Asian Pangasius catfish, tra, and basa, with no cross-reactions with any of the common fish and meat species or with the food additive proteins (bovine serum albumin, soy proteins, milk proteins, egg proteins, and gelatin) tested. MAb T7E10 recognized 2 antigenic proteins (molecular weight approximately 36 and 75 kDa) in raw and cooked tra and basa extracts, while T1G11 bound to several proteins (molecular weight between 13 and 18 kDa) in tra and basa extracts. Two other MAbs, F7B8 and F1G11, recognized a common protein (36 KDa) and cross-reacted with all the fish extracts tested and with several mammalian species. These MAbs can be employed individually or in combination in various formats of immunoassays for rapid identification of Pangasius catfish, either raw or cooked. They can also be used to study the biological, biochemical, and physiological aspects of thermal-stable antigenic proteins. This is the first study identifying these thermal-stable antigenic proteins present in Pangasius catfish as species-specific biomarkers.

  1. Assessment of mercury content in Panga (Pangasius hypophthalmus).

    Science.gov (United States)

    Rodríguez, María; Gutiérrez, Ángel J; Rodríguez, Natividad; Rubio, Carmen; Paz, Soraya; Martín, Verónica; Revert, Consuelo; Hardisson, Arturo

    2018-04-01

    Panga (Pangasius hypophthalmus), originating from Vietnam, is one of the most consumed fish because of its low cost, mild taste and presentation (fillets without skin and bones). Nevertheless, due to the high levels of contamination of the rivers where it lives, and to the fact that the panga is a predator, whereby it will accumulate a higher mercury concentration, the main objectives of the present study were to evaluate the toxic risk from mercury ingestion as a result of the consumption of this fish. A total of 80 frozen panga samples natural and marinade from different commercial establishments have been analyzed using cold vapour atomic absorption spectrophotometry (CV-AAS). The results obtained show a wide range of mercury concentrations between 0.10 and 0.69 mg/kg, with an average value of 0.22 mg/kg. In addition, it has been found that the average mercury concentration in the marinated presentation (0.18 mg/kg) is higher than that obtained in the natural panga (0.16 mg/kg). However, no significant differences were found between commercial establishments or in the presentation formats, with the conclusion that they did not influence the mercury content in the samples. Assuming a weekly consumption of 350 g of panga, the contribution rate to Tolerable Weekly Intake (TWI) of mercury (4 μg/kg bw/week) is 32% and 27.5% for women and men, respectively. Based on the results obtained in this study, an exhaustive control of the mercury content in this type of fish is necessary. In addition, a consumption restriction to children will be established. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Carbohydrate metabolism in catfish

    International Nuclear Information System (INIS)

    Saad, C.R.; Lovell, R.T.

    2002-01-01

    Radiolabeled (U- 14 C)-glucose was incorporated in diets and forced-fed to channel catfish and was observed for a 24 hour period. About 95% of fed labeled (U- 14 C)-glucose was absorbed by catfish, showing a high digestibility of glucose. The amounts of 14 C excreted over 24 h as carbon dioxide were 49% and amounts excreted in urine were 3.5%. The amount retained as protein, fat glycogen and other organic compounds were 8.2, 1.2, 6.5 and 32.1 % respectively, for the 24 hour period. The blood concentration of 14 C reached a maximum 2.5 hour after feeding (U- 14 C)-glucose, then gradually decreased. Based on tissue concentrations of 14 C, glycogen was an immediate storage site for absorbed glucose, but 14 C- glycogen in liver decreased rapidly. Glucose was quickly and heavily converted into triglyceride, indicating that fat is an important intermediate in the metabolism of glucose in channel catfish. 14 C-fat in the serum and liver were transferred to the adipose tissue in the muscle and mesentery about 10 hours after feeding. (Author)

  3. TAXONOMY AND GENETIC RELATIONSHIPS OF PANGASIIDAE, ASIAN CATFISHES, BASED ON MORPHOLOGICAL AND MOLECULAR ANALYSES

    Directory of Open Access Journals (Sweden)

    Rudhy Gustiano

    2007-12-01

    Full Text Available Pangasiids are economically important riverine catfishes generally residing in freshwater from the Indian subcontinent to the Indonesian Archipelago. The systematics of this family are still poorly known. Consequently, lack of such basic information impedes the understanding of the biology of the Pangasiids and the study of their aquaculture potential as well as improvement of seed production and growth performance. The objectives of the present study are to clarify phylogeny of this family based on a biometric analysis and molecular evidence using 12S ribosomal mtDNA on the total of 1070 specimens. The study revealed that 28 species are recognised as valid in Pangasiidae. Four genera are also recognized as Helicophagus Bleeker 1858, Pangasianodon Chevey 1930, Pteropangasius Fowler 1937, and Pangasius Valenciennes 1840 instead of two as reported by previous workers. The phylogenetic analysis demonstrated the recognised genera, and genetic relationships among taxa. Overall, trees from the different analyses show similar topologies and confirm the hypothesis derived from geological history, palaeontology, and similar models in other taxa of fishes from the same area. The oldest genus may already have existed when the Asian mainland was still connected to the islands in the southern part about 20 million years ago.

  4. Catfish Farmers Perception of Training Effectiveness of the ...

    African Journals Online (AJOL)

    ... terms of the communication methods used by the trainers ( x =1.83), clarity in ... In order to enhance the entrepreneurial skills of farmers involved in catfish ... Keywords: catfish production, entrepreneurial skills, training on catfish, West Africa

  5. 76 FR 10433 - Mandatory Inspection of Catfish and Catfish Products

    Science.gov (United States)

    2011-02-24

    ... defining ``catfish'' after reviewing the legislative and regulatory history and scientific classification... sold as whole gutted fish (head on), mainly to restaurants and institutions. Waste materials and... 1991 NJ Restaurant Salmonella 10 6 0 1999 FL Private Home Unknown 2 0 0 1999 FL Restaurant/Deli...

  6. Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

    NARCIS (Netherlands)

    Vischer, HF; Granneman, JCM; Linskens, MHK; Schulz, RW; Bogerd, J

    LH and FSH are heterodimeric glycoprotein hormones, composed of a common alpha-subunit non-covalently associated with a hormone-specific beta-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries.

  7. EMBRIOGENESIS DAN PERKEMBANGAN LARVA PATIN HASIL HIBRIDISASI ANTARA BETINA IKAN PATIN SIAM ( Pangasianodon hypophthalmus Sauvage, 1878 DENGAN JANTAN IKAN PATIN JAMBAL ( Pangasius djambal Bleeker, 1846 DAN JANTAN PATIN NASUTUS ( Pangasius nasutus Bleeker, 1863

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2011-08-01

    Full Text Available Pengembangan budidaya ikan patin jambal maupun ikan patin nasutus untuk memenuhi permintaan pasar ekspor patin daging putih sulit direalisasikan karena keterbatasan fekunditas dan pematangan gonad induk betinanya. Salah satu upaya yang dapat dilakukan untuk meningkatkan produktivitas patin daging putih adalah melalui hibridisasi, yakni hibridisasi antara betina patin siam dengan jantan patin jambal maupun jantan patin nasutus. Hal ini dikarenakan patin siam memiliki keunggulan fekunditas yang tinggi, sedangkan patin jambal maupun patin nasutus memiliki keunggulan daging yang putih. Oleh karena itu, perlu dilakukan penelitian untuk mengetahui karakteristik patin hibrida tersebut, termasuk pada tahap-tahap awal kehidupannya. Penelitian ini bertujuan untuk mengetahui karakteristik embriogenesis dan perkembangan ontogeni morfologis larva patin hibrida tersebut. Hasil penelitian ini menunjukkan bahwa embriogenesis patin hibrida siam-jambal dan hibrida siam-nasutus serupa, tetapi perkembangan patin hibrida siam-nasutus sedikit lebih cepat. Pada suhu 28oC-29oC, larva patin hibrida siam-nasutus mulai menetas 20 jam setelah fertilisasi, berukuran panjang total 3,34±0,14 mm, dengan kantung kuning telur berukuran 0,71±0,28 mm3 yang terserap 50% pada umur 24 jam dan relatif habis terserap pada umur 54 jam. Larva patin hibrida siam-jambal mulai menetas 21 jam setelah fertilisasi, berukuran panjang total 3,47±0,13 mm, dengan kantung kuning telur berukuran 0,42±0,08 mm3 yang terserap 50% pada umur 30 jam dan habis terserap pada umur 60 jam. Perkembangan larva kedua patin hibrida tersebut hingga menyerupai morfologi ikan patin dewasa juga relatif serupa, tetapi patin hibrida siam-nasutus menunjukkan keragaan pertumbuhan yang lebih bagus, menghasilkan heterosis berdasarkan pertambahan panjang total selama 10 hari masa pemeliharaan sebesar 20,20%, sedangkan pada patin hibrida siam-jambal sebesar -4,15%.

  8. Evaluation of stomach tubes and gastric lavage for sampling diets from blue catfish and flathead catfish

    Science.gov (United States)

    Waters, D.S.; Kwak, T.J.; Arnott, J.B.; Pine, William E.

    2004-01-01

    We compared the ability to extract all stomach contents by using stomach tubes or gastric lavage to sample diets from blue catfish Ictalurus furcatus and flathead catfish Pylodictus olivarus. Pulsed gastric lavage (PGL) removed a significantly greater proportion of stomach content mass (95.6%) from blue catfish than did stomach tubes (14.6%). Percent mass of flathead catfish contents removed with PGL (96.0%) was not significantly different from that removed with stomach tubes (86.9%). Based on the greater effectiveness of PGL for blue catfish, combined with a shorter mean time required per sample (69 versus 118 s) and the better preservation of extracted diet material, we recommend using PGL as a nonlethal technique to collect diet samples from large catfishes.

  9. The catfish genome database cBARBEL: an informatic platform for genome biology of ictalurid catfish.

    Science.gov (United States)

    Lu, Jianguo; Peatman, Eric; Yang, Qing; Wang, Shaolin; Hu, Zhiliang; Reecy, James; Kucuktas, Huseyin; Liu, Zhanjiang

    2011-01-01

    The catfish genome database, cBARBEL (abbreviated from catfish Breeder And Researcher Bioinformatics Entry Location) is an online open-access database for genome biology of ictalurid catfish (Ictalurus spp.). It serves as a comprehensive, integrative platform for all aspects of catfish genetics, genomics and related data resources. cBARBEL provides BLAST-based, fuzzy and specific search functions, visualization of catfish linkage, physical and integrated maps, a catfish EST contig viewer with SNP information overlay, and GBrowse-based organization of catfish genomic data based on sequence similarity with zebrafish chromosomes. Subsections of the database are tightly related, allowing a user with a sequence or search string of interest to navigate seamlessly from one area to another. As catfish genome sequencing proceeds and ongoing quantitative trait loci (QTL) projects bear fruit, cBARBEL will allow rapid data integration and dissemination within the catfish research community and to interested stakeholders. cBARBEL can be accessed at http://catfishgenome.org.

  10. The World Demand for Catfish Pangasius

    DEFF Research Database (Denmark)

    Nguyen, Thong Tien; Roth, Eva; Nielsen, Max

    2014-01-01

    flexibilities are less than 1. While there are many demand studies for other whitefish species and salmon, which are strongly competed by catfish in many markets, there is a lack of demand study for Pangasius catfish. Therefore, this study will supplement seafood demand literature and be meaningful for seafood......In this paper we present a world demand system for Pangasius catfish products. We use solely exporting data from Vietnam for estimating a non-linear Almost Ideal Demand System because Vietnam accounts for more than 90% catfish export value of the world and the products exported are mostly...... in filleted form. The demand system includes seven equations representing for most important markets that are ASEAN & EAST ASIA, NORTH AMERICA, OCEANIA, RUSIAN & EASTERN EU, SOUTH & CENTRAL AMERICA, WESTERN EU, and ROW (rest of the world) markets. The monthly data are updating from January 2007 to March 2014...

  11. Induced propagation of African clariid catfish, Heterobranchus ...

    African Journals Online (AJOL)

    SERVER

    2007-12-03

    Dec 3, 2007 ... Our present study which compared the effect of synthe- tic (ovaprim) .... was maintained with RESUN LP- 100 low noise air - pump. A sample of ..... reproduction and fingerling production of the African catfish Clarias gariepinus ...

  12. Organoleptic Characteristics and Chemicals Ilabulo Catfish Fortification

    Directory of Open Access Journals (Sweden)

    Rita Marsuci Harmain

    2017-08-01

    Full Text Available Diversification of traditional food ilabulo made from raw catfish (Pangasius sp. has the potential to be developed in Gorontalo province to substitute chicken viscera. The research aimed to make ilabulo substitute the raw material of chicken viscera with the catfish fortified Kappaphycus alvarezii seaweed and catfish bone meal and to determine the organoleptic and chemical characteristics of ilabulo catfish fortification. Fortified treatment is K. alvarezii  and catfish bone (5 dan 10% ,  B (10 and 15% and  C (15 dan 20%. The organoleptic analysis used a hedonic scale of favorite criteria on the appearance, texture, color, flavor, and taste. The results of organoleptic analysis continued with Bayes test. The chemical analysis used the Association of Official Analytical Chemist method. The result of the hedonic characteristic of ilabulo catfish fortification was on the appearance  neutral criteria – like (5.53–7.03, texture neutral criteria – rather like (5.8–7.1, aroma rather like (6.3–6.73, color neutral – like (6.1–7.03 and taste (6.07–6.53 neutral criteria – rather like. The result of Bayes test obtained by a texture of importance value 5, appearance importance value 5, aroma of importance value 4, color of interest value 3 and taste of importance value 2. Characteristic of ilabulo culture of selected fortified catfish that was the fortification of K. alvarezii seaweed 15% and catfish  bone flour (20% (fortification C obtained by water content 56.46%, ash 11.54%, protein 7.78%, fat 8.91%, coarse fiber 0.61%, carbohydrate 22.07% and calcium 0.315 %.

  13. Catfish stings: A report of two cases

    Directory of Open Access Journals (Sweden)

    Gholamali Dorooshi

    2012-01-01

    Full Text Available Venomous catfish stings are a common environment hazard worldwide. Although these stings are often innocuous, significant morbidity may result from stings, including severe pain, retained foreign bodies, infection, respiratory compromise, arterial hypotension, and cardiac dysrhythmias. Treatment included hot water immersion, analgesia, wound exploration, and prophylactic antibiotics. In this article, two cases of stings by catfish referred to the poison center of Noor Hospital, Isfahan University of Medical Sciences and their treatments have been reported.

  14. Defeating diplostomoid dangers in USA catfish aquaculture.

    Science.gov (United States)

    Overstreet, Robin M; Curran, Stephen S

    2004-06-01

    Diplostomoid digenean metacercariae have caused widescale mortalities of channel catfish, Ictalurus punctatus (Rafinesque), at aquaculture farms in Louisiana, Mississippi and Arkansas, USA. Originally, based on a tentative diagnosis, the industry considered the primary harmful agent to be an introduced species from Europe, Bolbophorus confusus (Krause, 1914), frequently reported from the American white pelican, Pelecanus erythrorhynchos Gmelin. Our group has now shown, using ITS 1-2 plus three more-conservative gene fragments, that two sympatric species of Bolbophorus exist in the American white pelican. One, B. damnificus Overstreet et Curran, 2002, infects the musculature of catfish, and the other, probably not B. confusus, does not infect catfish. However, at least four other pathogenic diplostomoids and a clinostomoid infect the catfish, and they use at least four different snail hosts, including the planorbids Planorbella trivolvis (Say) and Gyraulus parvus (Say), the physid Physella gyrina (Say) and a lymnaeid. Two metacercariae, B. damnificus and Bursacetabulus pelecanus Dronen, Tehrany et Wardle, 1999, infect the catfish and mature in the pelican; two others, Austrodiplostomum compactum (Lutz, 1928) and Hysteromorpha cf. triloba (Rudolphi, 1819), mature in cormorants; one, Diplostomum sp., matures in seagulls and at least one, Clinostomum marginatum (Rudolphi, 1819), matures in herons, egrets and other wading birds. Consequently, management of catfish ponds relative to digenean infections requires considerable biological information on the fish, bird, and snail hosts as well as the parasites.

  15. Profitability analysis of catfish farming in Suleja local government ...

    African Journals Online (AJOL)

    The problem of profitability and scale of production of catfish has not been properly addressed. This study was conducted in Suleja Local Government Area of Niger State to assess the profitability of catfish production. Forty (40) catfish farmers were selected from the study area using simple random sampling techniques.

  16. Profitability analysis of catfish production in Kaduna state, Nigeria ...

    African Journals Online (AJOL)

    This study examined the profitability of catfish production in Kaduna State, Nigeria. The objective was to determine the costs and returns in catfish production and thus the profit. Data for the study were collected through the use of well-structured questionnaire. A total of 60 catfish farmers were randomly selected from four ...

  17. PENAMBAHAN BAHAN PEMBENTUK GEL DALAM PEMBUATAN SURIMI DARI IKAN PATIN (Pangasius hypophthalmus

    Directory of Open Access Journals (Sweden)

    Theresia Dwi Suryaningrum Dwi Suryaningrum

    2014-06-01

    Full Text Available Kajian tentang pengolahan surimi dari ikar patin (P. hypophthalmus dengan menggunakan karaginan atau kalsium laktat 0,05% sebagai bahan pembentuk gel telah dilakukan. Pengamatan dilakukan selama proses pengolahan dan mutu surimi yang dihasilkan. Mutu surimi yang diamati adalah uji daya lipat, kekuatan gel, viskositas, derajat putih, komposisi proksimat, kandungan bakteri, serta uji organoleptik. Hasil penelitian menunjukkan bahwa pengolahan daging ikan patin menjadi surimi menghasilkan rendemen sebesar 23,03% dengan kadar lemak yang masih cukup tinggi yaitu sebesar 13,14% (bk, derajat putih 27,90 serta mengandung benda asing berupa serpihan kulit yang jumlahnya berkisar antara 11-16 serpihan/100 cm². Penambahan karaginan atau kalsium laktat sedikit meningkatkan rendemen surimi dibandingkan dengan kontrol. Penambahan karaginan atau kalsium laktat tidak berpengaruh terhadap daya lipat surimi, yaitu tidak retak ketika dilipat menjadi 4 (grade AA. Surimi yang diberi perlakuan penambahan kalsium laktat menghasilkan derajat putih dan kekentalan yang lebih tinggi dibandingkan dengan surimi yang diberi perlakuan karaginan dan kontrol. Kekuatan gel yang diperoloh dalam penelitian ini berkisar antara 978,93-1095,25 g/cm² dengan kekuatan gel terbaik diperoleh pada surimi yang ditambah karaginan. Penambahan bahan pembentuk gel tidak berpengaruh terhadap kadar air, abu, protein maupun lemak produk. Uji sensori menunjukkan bahwa perlakuan yang diberikan tidak berpengaruh terhadap warna dan bau surimi, tetapi penambahan karaginan menghasilkan surimi dengan nilai tekstur paling tinggi sedangkan penambahan kalsium laktat menghasilkan surimi dengan nilai penampakan paling tinggi. Berdasarkan uji kesukaan, surimi yang diberi penambahan kalsium laktat lebih disukai oleh panelis dibandingkan dengan surimi yang diberi perlakuan karaginan.

  18. Microbial ecology of Vietnamese Tra fish (Pangasius hypophthalmus) fillets during processing.

    Science.gov (United States)

    Tong Thi, Anh Ngoc; Noseda, Bert; Samapundo, Simbarashe; Nguyen, Binh Ly; Broekaert, Katrien; Rasschaert, Geertrui; Heyndrickx, Marc; Devlieghere, Frank

    2013-10-15

    There are numerous factors that can have an impact on the microbial ecology and quality of frozen Pangasius hypophthalmus fillets during processing in Vietnam. The presence of spoilage bacteria along the processing line can shorten the shelf-life of thawed frozen fish products. Therefore, the spoilage microbiota throughout the processing chain of two companies (BC: large scale factory, chlorine-based process, BW: large scale factory, water-based process and SC: small scale factory, chlorine-based process) was identified by culture-dependent techniques and 16S rRNA gene sequencing. The microbiological counts were observed to be insignificantly different (p>0.05) between BC and BW. Surprisingly, chlorine treated fillets from the SC line were revealed to have significantly higher microbial counts than potable water treated fillets at BW line. This was determined to be a result of temperature abuse during processing at SC, with temperatures even greater than 10 °C being recorded from skinning onwards. On the contrary, the microbiota related to spoilage for BC and BW lines was determined by 16S rRNA gene sequencing to be more diverse than that on the SC line. A total of 174 isolates, 20 genera and 38 species were identified along the processing chains. The genera Aeromonas, Acinetobacter, Lactococcus and Enterococcus were prevalent at various processing steps on all the processing lines evaluated. A diverse range of isolates belonging to the Enterobacteriaceae such as Providencia, Shigella, Klebsiella, Enterobacter and Wautersiella were isolated from fillets sampled on the SC line whereas Serratia was only observed on fillets sampled on the BC and BW lines. The results can be used to improve Good Manufacturing Practices for processed Pangasius fillets and to select effective measures to prolong the shelf-life of thawed Vietnamese Pangasius fillets products. © 2013.

  19. Socio-economics of catfish production in Port Harcourt, Rivers State ...

    African Journals Online (AJOL)

    The appraisal of profitability of catfish production in Port Harcourt metropolis Nigeria was carried out by identifying socio-economic characteristics of catfish farmers, cost and returns of catfish farming and the basic problems hindering effective catfish production. Thirty catfish farmers were randomly selected; administered a ...

  20. Critical oxygen tension and the effect of hypoxia on the oxygen consumption of the striped catfish, Pangasius hypophthaimos (Pangasiidae)

    DEFF Research Database (Denmark)

    Lefevre, S.; Bayley, Mark; Wang, Tobias

    2008-01-01

    The striped catfish (Pangasius hypophthalmus) is an air-breathing teleost that uses a modified swim bladder for aerial gas exchange. Pangasius is of enormous importance for aquaculture industry in the Mekong Delta(Vietnam), but little is known about its physiology. We have initiated a series...... consumption (VO2), measured with intermittent closed respirometry, was 67.8 ± 5.1 mLO2/kg/h when the fish were maintained without access to air at 27 °C. The critical oxygen tension (Pcrit) at these conditions was 57.9 ± 8.9 mmHg (N = 7). The metabolic response to aquatic hypoxia was studied in fish subjected....... The ontogenetic effect of environmental PO2 on metabolism is currently under investigation with fish being reared in 30%, 60% and 100% saturation. Data on resting VO2and Pcrit will be presented and discussed for these animals....

  1. The growth performance of F1 transgenic mutiara catfish

    Science.gov (United States)

    Iskandar; Buwono, I. D.; Agung, M. U. K.

    2018-04-01

    The growth of catfish (African or Sangkuriang strain) these days is tend to decreased. One of the solutions due to this problem is to improve the genetics of growth using transgenesis technology, toward more profitable. The specific objective of the research is to detect the transmission of exogenous GH (African catfish GH inserts) inside the F1 transgenic Mutiara catfish using PCR (Polymerase Chain Reaction) method and to evaluate the growth performance of transgenic Mutiara catfish made using the parameters of feed conversion (FCR = Feed Conversion Ratio). Transgenic catfish (strain mutiara) F0 and F1 carried African catfish GH (600 bp) can be produced. Superiority characters of transgenic catfish represented heritability (h2 ) and heterosis (H), indicating that the offspring of hybrid F1 transgenic mutiara catfish had phenotypes rapid growth (h2 = 17.55 % and H = 42.83 %) compared to non-transgenic catfish (h 2 = 10.07 % and H = 18.56 %). Evaluation of the efficiency of feed use parameters feed conversion ratio, shows that F1 transgenic mutiara catfish (FCR = 0.85) more efficient in converting feed into meat.

  2. Monogeneans from Pangasiidae (Siluriformes in Southeast Asia: III. Five new species of Thaparocleidus Jain, 1952 (Ancylodiscoididae from Pangasius bocourti, P. djambal and P. hypophthalmus

    Directory of Open Access Journals (Sweden)

    Pariselle A.M.

    2002-09-01

    Full Text Available The examination of gill parasites from Pangasius bocourti Sauvage, 1880; P. djambal Bleeker, 1846; P. hypophthalmus (Sauvage, 1878 and P. gigas Chevey, 1930 (Siluriformes, Pangasiidae revealed the presence of seven species of Monogenea among which five are considered new species. They all belong to Thaparocleidus Jain, 1952 (Ancylodiscoididae as defined by Lim (1996 and Lim et al. (2001. . P. bocourti: T. combesi n. sp.: T. komarudini n. sp. and T. vietnamensis n. sp. . P. djambal: T. caecus (Mizelle & Kritsky, 1969, T. combesi n. sp., T. euzeti n. sp., T. komarudini n. sp. and T. sadilii n. sp. . P. hypophthalmus: T. caecus, T. siamensis (Lim, 1990 and T. vietnamensis n. sp. . P. gigas : no Monogenea were found on this host species.

  3. Oxygen requirement of separated hybrid catfish eggs

    Science.gov (United States)

    Channel catfish egg masses require hatchery water with over 7.8 ppm dissolved oxygen at 80° F (95% air saturation) to maintain maximum oxygen consumption as they near hatching. This concentration is called the critical oxygen requirement by scientists but for the purpose of this article we will call...

  4. Cellular biomarker responses of bagrid catfish, Chrysichthys ...

    African Journals Online (AJOL)

    An assessment of the pollution status of Agboyi creek, a water body associated with various anthropogenic activities was carried out in order to determine responses induced in Catfishes, Chrysichthys nigrodigitatus inhabiting it. Cellular biomarkers of stress including the antioxidative stress enzyme, catalase (CAT), lipid ...

  5. Effect of L-Ascorbyl-2-Phosphate Magnesium as a Vitamin C Source in Different Doses on Growth of Patin Pangasius Hypophthalmus Fingerlings

    Directory of Open Access Journals (Sweden)

    D. Jusadi

    2007-01-01

    Full Text Available This study was aimed to determine optimum dose of L-Ascorbyl-2-Phosphate Magnesium in the diet of patin, Pangasius hypophthalmus fingerlings.  Five isoprotein and isocaloric diets containing different dose of L-Ascorbyl-2-Phosphate Magnesium, i.e. 0, 25, 75 and 100 mg/kg diet were used in this experiment.  Fish with an average size of 5.00±0.11 g were maintained at a density of 15 fish per aquarium.  Fish were fed three times a day at satiation, for 40 days.  The results of study shows that vitamin C content in fish body increased as the vitamin C level of the diet increased.  Similar pattern to vitamin C content was also found in protein retention, lipid retention, daily growth rate, and feed efficiency (p Keywords: vitamin C, patin, Pangasius hypophthalmus.   ABSTRAK Penelitian ini bertujuan untuk menentukan kadar optimum L-Ascorbyl-2-Phosphate Magnesium dalam pakan ikan patin, Pangasius hypophthalmus ukuran sejari. Lima macam pakan isoprotein dan isokalori mempunyai kandungan L-Ascorbyl-2-Phosphate Magnesium berbeda, berturut-turut 0, 25, 50, 75 dan 100 mg/kg pakan telah digunakan dalam penelitian ini. Benih berukuran rata-rata 5,00±0,11 g dipelihara dalam akuarium dengan kepadatan 15 ekor per akuarium. Ikan diberi pakan tiga kali sehari, secara at satiation, selama 40 hari. Hasil penelitian menunjukkan bahwa kadar vitamin C tubuh meningkat sejalan dengan kadar vitamin C pakan. Demikian pula retensi protein, retensi lemak laju pertumbuhan harian, efisiensi pakan mengikuti pola yang sama seperti kandungan vitamin C tubuh (p Kata kunci: vitamin C, ikan patin, Pangasius hypophthalmus.

  6. Inactivation of Salmonellae in Frozen Catfish by Gamma Irradiation

    International Nuclear Information System (INIS)

    Nouchpramoon, Kovit; Amsiri, Jarurat

    2003-06-01

    The effect of gamma irradiation on salmonellae viability in frozen catfish was investigated using fresh cut of catfish artificially contaminated with stationary phase cells of salmonellae, frozen at-18 οC and irradiated with does ranging from 0.0 to 2.4 kGy. The D 10 values for ten serovars of salmonellae ranged from 0.47 to 0.77 kGy. Salmonella Enteritidis was the most resistant serovars found in frozen catfish. Dosage at 2.5 kGy would be sufficient to kill 10 3 . 2 Salmonella Enteritidis that may occasionally present in frozen catfish

  7. Induced spawning, survival and growth of an African catfish hybrid ...

    African Journals Online (AJOL)

    Induced spawning, survival and growth of an African catfish hybrid (female Clarias gariepinus and male Clarias anguillaris ) fingerlings relative to their parental species in the mount Cameroon region.

  8. Non specific immune response in the African catfish ...

    African Journals Online (AJOL)

    Non specific immune response in the African catfish, Heterobranchus longifilis fed diets fortified with ethanolic extracts of selected traditional medicinal plants and disease resistance against Pseudomonas aeruginosa.

  9. Catfish science: Status and trends in the 21st century

    Science.gov (United States)

    Kwak, Thomas J.; Porath, Mark T.; Michaletz, Paul H.; Travnichek, Vincent H.

    2011-01-01

    Catfish science, the study of the fish order Siluriformes, is a diverse and expanding field in terms of advances and breadth of topics. We compiled literature from primary fisheries journals as an index of interest and advances in catfish science to examine temporal trends in the field. The number of catfish scientific publications varied over the past century with strong peaks during 1975–1979 and 2005–2010, which may be the result of interactive scientific and societal influences. Catfish biology was the predominant publication topic until the late 1990s, when ecology, techniques, and management publications became more prevalent. Articles on catfish ecology were most numerous in both the first and second international catfish symposia, but publications on techniques and conservation were more numerous in the second catfish symposium than the first. We summarize the state of knowledge, recent advances, and areas for future attention among topics in catfish science, including sampling and aging techniques, population dynamics, ecology, fisheries management, species diversity, nonnative catfish, and human dimensions, with an emphasis on the gains in this second symposium. Areas that we expect to be pursued in the future are development of new techniques and validation of existing methods; expansion of research to less-studied catfish species; broadening temporal, spatial, and organizational scales; interdisciplinary approaches; and research on societal views and constituent demands. Meeting these challenges will require scientists to span beyond their professional comfort zones to effectively reach higher standards. We look forward to the coming decade and the many advances in the conservation, ecology, and management of catfish that will be shared.

  10. Oxygen requirements of separated hybrid catfish female Ictalurus punctatus male I. furcatus eggs

    Science.gov (United States)

    Channel catfish Ictalurus punctatus egg masses require ambient water with over 95% air saturation to maintain maximum oxygen consumption as they near hatch. Since hybrid catfish eggs (channel catfish ' X blue catfish I. furcatus ') are often kept separated after fertilization by the addition of full...

  11. PENGGUNAAN EKSTRAK DAUN SIRIH (Piper betle Linn UNTUK MENGHAMBAT BAKTERI Aeromonas hydrophila DAN TOKSISITASNYA PADA IKAN PATIN (Pangasius hypophthalmus (THE USE OF PIPER BETLE LINN EXTRACT ON AEROMONAS HYDROPHILA TO OBSTRUCT AND THE TOKSISITY TO PANGASIUS HYPOPHTHALMUS

    Directory of Open Access Journals (Sweden)

    Siti Aisiah

    2016-06-01

    Full Text Available Tujuan penelitian ini untuk mengetahui   daya  hambat  daun  sirih   yang  paling  besar  terhadap  bakteri Aeromonas Hydrophila, mengetahui konsentrasi minimal ekstrak daun sirih yang mampu menghambat pertumbuhan bakteri  A. hydrophila  dan mengetahui toksisitas konsentrasi efektif dari  ekstrak daun sirih terhadap ikan patin. Rancangan percobaan yang digunakan untuk  uji toksisitas  adalah rancangan acak lengkap, terdiri dari 4 perlakuan yaitu A  = Ikan disuntik dengan ekstrak daun sirih konsentrasi 75%, B  = Ikan disuntik dengan ekstrak daun sirih konsentrasi 25%, C  = Kontrol positif (ikan disuntik dengan akuades steril dan D  = Kontrol negatif (ikan tidak disuntik, diulang 3 kali. Hasil uji sensitivitas antibakteri daun sirih yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila adalah ekstrak daun sirih-metanol. Pengujian MIC menunjukkan bahwa ekstrak daun sirih-metanol memiliki daya hambat minimal 25 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan patin dengan konsentrasi 75% dan 25% menunjukkan bahwa mortalitas yang terjadi tidak mencapai 50%. Pengamatan hematologis pada masing-masing perlakuan menunjukkan hasil yang berpengaruh tidak nyata terhadap kesehatan ikan patin. Parameter kualitas air pada penelitian ini yaitu, , kadar oksigen terlarut,  pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan ikan patin. This research was aimed a finding the part of Piper betle Linn  which had the biggest resistance to Aeromonas hydrophila bacteria and to know the minimal concentrate which could obstruct the growth of A. hydrophila  bacteria and to know effective concentrate toxicity of P. betle Linn  to Pangasius hypophthalmus.  The random sampling used proportionate stratified random sampling. In toxicity test, it had be done 4 treatment, which was given to fish, those were : A = fish was injected with 25 % concentrate of extract  P

  12. Determinação de idade e crescimento do mapará (Hypophthalmus marginatus na Amazônia Central Age and growth of mapará (Hypophthalmus marginatus in the Central Amazon

    Directory of Open Access Journals (Sweden)

    Leocy Cutrim

    2005-01-01

    Full Text Available O presente trabalho visa contribuir para a conservação e manejo do mapará (Hypophthalmus marginatus, um importante recurso pesqueiro de exportação do Amazonas para o qual é necessário conhecer informações essenciais de sua dinâmica de populações. Para contribuir à esta finalidade, o presente trabalho estabeleceu como objetivo identificar o par de otólito mais adequado para leitura de anéis etários, determinar os parâmetros de crescimento do mapará (H. marginatus, Amazônia Central, Amazonas - Brasil. O trabalho foi efetuado através da análise de otólitos coletados no período de dezembro de 1996 á agosto de 1997. Os otólitos escolhidos foram os asteriscus, sendo a leitura efetuada em lupa estereoscópica com monitor acoplado. As marcas de crescimento foram validadas por meio da análise do incremento marginal relativo, sendo encontrado dois anéis/ano. Os valores estimados para os parâmetros no período foram L¥ = 52,63 cm; k = 0,555 ano; to = -0,029 e M = 0,552. O ciclo hidrológico e comportamento reprodutivo estão relacionados com a marcação de anéis etários.The present work seeks to contribute for the conservation and management of the mapará(Hypophthalmus marginatus, an important export fishing resource of Amazonas for which is necessary to know essential information of its population dynamics. To contribute to this purpose, the present work established as objective to identify a structure for reading of age rings, to determine the parameters of growth of the mapará (H. marginatus, Central Amazon, Amazonas - Brazil. The work was made through the otoliths analysis collected in the period of December of 1996 to August of 1997. The otolith chosen for reading was the asteriscus, being the reading made in a stereomicroscope with coupled monitor. The mark structures were validated by means of the analysis of the relative marginal increment, being found two rings. The values estimated for the parameters in the

  13. GWAS analysis using interspecific backcross progenies reveals superior blue catfish alleles responsible for strong resistance against enteric septicemia of catfish.

    Science.gov (United States)

    Tan, Suxu; Zhou, Tao; Wang, Wenwen; Jin, Yulin; Wang, Xiaozhu; Geng, Xin; Luo, Jian; Yuan, Zihao; Yang, Yujia; Shi, Huitong; Gao, Dongya; Dunham, Rex; Liu, Zhanjiang

    2018-05-08

    Infectious diseases pose significant threats to the catfish industry. Enteric septicemia of catfish (ESC) caused by Edwardsiella ictaluri is the most devastating disease for catfish aquaculture, causing huge economic losses annually. Channel catfish and blue catfish exhibit great contrast in resistance against ESC, with channel catfish being highly susceptible and blue catfish being highly resistant. As such, the interspecific backcross progenies provide an ideal system for the identification of quantitative trait locus (QTL). We previously reported one significant QTL on linkage group (LG) 1 using the third-generation backcrosses, but the number of founders used to make the second- and third-generation backcross progenies was very small. Although the third-generation backcross progenies provided a greater power for fine mapping than the first-generation backcrosses, some major QTL for disease resistance may have been missing due to the small numbers of founders used to produce the higher generation backcrosses. In this study, we performed a genome-wide association study using first-generation backcrosses with the catfish 690 K SNP arrays to identify additional ESC disease resistance QTL, especially those at the species level. Two genomic regions on LG1 and LG23 were determined to be significantly associated with ESC resistance as revealed by a mixed linear model and family-based association test. Examination of the resistance alleles indicated their origin from blue catfish, indicating that at least two major disease resistance loci exist among blue catfish populations. Upon further validation, markers linked with major ESC disease resistance QTL should be useful for marker-assisted introgression, allowing development of highly ESC resistant breeds of catfish.

  14. Treating catfish diseases: walking the line between excess and moderation

    Science.gov (United States)

    Cost savings by using a cheaper disease treatment will increase profitability of any catfish farm. This invited producer presentation will discuss costs savings using copper sulfate in catfish production and a summation of our research, specifically in the hatchery. Copper sulfate is not approved ...

  15. Rheological and Functional Properties of Catfish Skin Protein Hydrolysates

    Science.gov (United States)

    Catfish skin is an abundant and underutilized resource that can be used as a unique protein source to make fish skin hydrolysates. The objectives of this study were to: isolating soluble and insoluble proteins from hydrolyzed catfish skin and study the chemical and functional properties of the prote...

  16. Stress in African catfish (clarias gariepinus) following overland transportation

    NARCIS (Netherlands)

    Manuel, R.; Boerrigter, J.; Roques, J.; Heul, van der J.W.; Bos, van den R.; Flik, G.; Vis, van de J.W.

    2014-01-01

    Of the many stressors in aquaculture, transportation of fish has remained poorly studied. The objective of this study was therefore to assess the effects of a (simulated) commercial transportation on stress physiology of market-size African catfish (Clarias gariepinus). Catfish weighing

  17. Profit efficiency among catfish farmers in Benue state, Nigeria | Tsue ...

    African Journals Online (AJOL)

    The study examined profit efficiency among catfish farmers in Benue State of Nigeria using a stochastic profit frontier approach. A multi-stage sampling technique was used to collect data from 143 catfish farmers through a well structured questionnaire. The study used a Cobb-Douglas stochastic profit frontier function to ...

  18. Growth and Survival of Catfish ( Clarias anguillaris ) Juveniles Fed ...

    African Journals Online (AJOL)

    Juveniles of catfish, Clarias anguillaris (mean weight, 119.8g) were fed unconventional diets for ten weeks in outdoor hapas (net cages, 1m3 dimension) and the growth responses and feed utilization by the juveniles were compared. The experimental diets were (i) live maggots, (ii) live tilapia fry, (iii) commercial catfish feed ...

  19. PENGARUH BERBAGAI RASIO ENERGI PROTEIN PADA PAKAN ISO PROTEIN 30% TERHADAP KINERJA PERTUMBUHAN BENIH IKAN PATIN (Pangasius hypophthalmus

    Directory of Open Access Journals (Sweden)

    Mas Bayu Syamsunarno

    2011-04-01

    Full Text Available Tujuan percobaan ini adalah untuk menentukan rasio energi protein optimum yang menghasilkan pertumbuhan maksimum benih ikan patin (Pangasius hypophthalmus. Percobaan menggunakan 5 (lima pakan iso protein dengan rasio energi protein berbeda, yaitu: 8,5; 9,0; 9,5; 10,0; dan 10,5 kkal DE/g protein. Benih patin berukuran 1,84±0,02 g ditebar secara acak ke dalam 15 akuarium (50 cm x 40 cm x 35 cm dengan kepadatan 20 ekor per akuarium. Benih ikan patin tersebut diberi pakan uji dua kali sehari sekenyangnya (satiation selama 40 hari. Hasil percobaan menunjukkan bahwa kandungan protein tubuh tertinggi dihasilkan oleh pakan dengan rasio energi protein 9,0 kkal DE/g protein, sedangkan lemak tubuh terendah dicapai oleh perlakuan 8,5 kkal DE/g protein. Namun, protein karkas adalah sama untuk perlakuan 8,5–9,5 kkal DE/g protein dan kandungan lemak karkas terendah dicapai oleh 8,5 kkal DE/g protein. Konsumsi pakan, retensi protein, dan pertumbuhan tertinggi dihasilkan oleh pakan dengan rasio energi protein 9,0 kkal DE/g protein (P0,05. Oleh karena itu, kandungan optimum rasio energi protein 9,0 kkal DE/g protein memberikan pertumbuhan tertinggi pada benih ikan patin

  20. Morphological description of larvae of the Mapará Hypophthalmus edentatus (Spix (Osteichthyes, Hypophthalmidae in the Itaipu reservoir (Parana River, Brazil

    Directory of Open Access Journals (Sweden)

    Keshiyu Nakatani

    1998-01-01

    Full Text Available The ontogenetic aspects of larvae of Hypophthalmus edentatus (Spix, 1829 from the ichthyoplankton samples collected in the Itaipu reservoir between March 1988 and April 1990 were analysed. Sample were caught by 0.5 mm mesh conic-cylindrical plankton net. Larvae of H. edentatus were identified and described as Siluriforms from the morphological and morphometric development series. They have a small to medium-sized head, long body, small eyes, little pigmentation, mandibular barbules and 53 to 56 myomeres. High values obtained with correlation coefficient (r > 0.97; p < 0.001 show that growth was proportional in different parts of the body.

  1. Investigation of antioxidant potential of peptide fractions from the Tra Catfish by-product-derived hydrolysate using Alcalase® 2.4 L FG

    Science.gov (United States)

    Vo, Tam D. L.; Chung, Duy T. M.; Doan, Kien T.; Le, Duy T.; Trinh, Hung V.

    2017-09-01

    In this study, the antioxidant capacity of peptide fractions isolated from the Tra Catfish (Pangasius hypophthalmus) by-product-derived proteolysate using ultrafiltration centrifugal devices with 5 distinct molecular-weight cutoffs (MWCOs) of 1 kDa, 3 kDa, 5 kDa, 10 kDa, and 30 kDa was investigated. Firstly, the chemical composition of the Tra Catfish by-products was analyzed. The result showed that the Tra Catfish by-products contained 58.5% moisture, 33.9% crude protein, 50.1% crude lipid and 15.8% ash (on dry weight basis). Secondly, the effects of hydrolysis time, enzyme content on the antioxidant potential of the proteolysate were studied using DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method (DPPH• SM) and FRAP (Ferric Reducing Antioxidant Potential) method. Alcalase® 2.4 L FG was used for hydrolysis. The result of antioxidant activity of the hydrolysate showed that the 50% DPPH• inhibition concentration (IC50) of the hydrolysate reached about 6775 µg/mL which was 1645-fold higher than that of vitamin C and 17-fold higher than that of BHT (ButylatedHydroxytoluene) with the degree of hydrolysis (DH) of the hydrolysate of 14.6% when hydrolysis time was 5 hours, enzyme/substrate (E/S) ratio was 30 U/g protein, hydrolysis temperature was 55°C, and pH was 7.5. The antioxidant potential of hydrolysate using FRAP method reached about 52.12 µM Trolox equivalent which was 53-fold and 18-fold lower than those of vitamin C and BHT, respectively, when the hydrolysis time was 5 h, enzyme/substrate ratio was 30 U/g protein, temperature was 500C, and pH level was 8. Next, the proteolysate was further fractionated using MWCOs of 1 kDa, 3 kDa, 5 kDa, 10 kDa, and 30 kDa and the peptide fractions were investigated for their antioxidant activity. The result showed that the <1 kDa fraction showed strongest antioxidant activity with the IC50 of 1313.31 ± 50.65 µg/mL and FRAP value of 906.90 ± 44.32 µM Trolox equivalent. The second strongest fraction

  2. Transgene transmission in South American catfish (Rhamdia quelen ...

    Indian Academy of Sciences (India)

    Prakash

    in this study was to evaluate different sperm-mediated gene transfer (SMGT) methods to obtain transgenic silver catfish. .... by the critical point method, they were observed under a ..... protein is important for the maintenance of sperm quality in.

  3. Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

    Science.gov (United States)

    The mucosal barriers of catfish (Ictalurus spp.) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, including nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catf...

  4. 104 Key words: Moringa, marinade, bacteria, fungi, catfish, smoke ...

    African Journals Online (AJOL)

    Osondu

    2013-01-16

    Jan 16, 2013 ... spoilage thus limiting economic loss and possible heath risk to consumers. Key words: Moringa, marinade, bacteria, fungi, catfish, smoke-dried. Introduction ..... were reared because E. coli is an indicator organism and its ...

  5. The Growth of Catfish (Clarias Gariepinus) with Aquaponic System

    OpenAIRE

    Ridho, Ari; Tang, Usman M; Putra, Iskandar

    2014-01-01

    The research was conducted in May 2013 at the Fish Hatchery and Breeding Laboratory Fisheries and Marine Science Faculty, Riau University. The aim of the research to know the productivity of catfish (Clarias gariepinus) with aquaponic system. The experimental methode with two treatments was applied in the research namely aquaponic and non-aquaponic. The result indicated that aquaponic have differential on growth of catfish (Clarias gariepinus). The best result was achieved by non-aquaponic. T...

  6. Xeroradiographic and radiographic anatomy of the channel catfish, Ictalurus punctatus

    International Nuclear Information System (INIS)

    Smith, S.A.; Smith, B.J.

    1994-01-01

    The purpose of this study was to provide an anatomic reference for the channel catfish (Ictalurus punctatus) using xeroradiography† and conventional radiography. The entire body of three adult fish was radiographed using standard xeroradiographic and conventional radiographic techniques. Two xeroradiographs and their corresponding conventional radiographs were selected, and the xeroradiographs labeled to illustrate the normal skeletal and soft-tissue anatomy of the channel catfish

  7. Analysis of Effective Broodstock Management and Breeding of Patin Siam (Pangasius hypophthalmus in BBAT Jambi

    Directory of Open Access Journals (Sweden)

    Mimid A. Hamid

    2009-01-01

    Full Text Available Aiming to analyze biological character of reproduction and to realize effective breeding activity, annual patin breeding activity 2006 was examined in JFADC Indonesia. Females of two generations, strain of 2001 and 2003 were induced during the year.  Various parameters, such as body weight, collected egg weight, number of 1g egg, harvested larvae, were recorded in every trial of breeding. After collecting all of data in 2006, correlation of productivity with fish size and age were analyzed integrating data. All female were also identified by using PIT tag system and alteration of maturity was monitored monthly, observing development of abdomen, from January to June. Accumulated parameters during the year showed negative correlation of egg somatic index (ESI with fish body weight.  Furthermore, hatching rate showed declined tendency as increasing body weight, suggesting less productivity by too-big female. All of elder female performed low productivity due to the low reading of some parameters such as ESI, hatching rate, larvae number/kg. Proportion of matured female was quite high from January until April. Individual monitoring of maturation showed quick reproduction of gonad in 2 month, from January to March, suggesting active reproductive stimulation on this season. Other many females also performed high gonadal maturity and its duration until April in many case. However, maturation has decreased suddenly on May and low maturation had lasted until November. It suppose due to the seasonal alteration of precipitation and remains as awkward issue for stable patin production in Jambi. Keywords: breeding, Asian catfish, egg somatic index, maturation   ABSTRAK Evaluasi kegiatan pembenihan patin siam Tahun 2006 di BBAT Jambi Indonesia dilakukan dengan tujuan untuk analisa karakter biologi reproduksi ikan patin siam dan untuk mewujudkan kegiatan pembenihan yang efektif.  Betina  dari 2 generasi yaitu generasi 2001 dan 2003 dipijahkan selama Tahun

  8. Verification of otolith identity used by fisheries scientists for aging channel catfish

    Science.gov (United States)

    Long, James M.; Stewart, David R.

    2010-01-01

    Previously published studies of the age estimation of channel catfish Ictalurus punctatus based on otoliths have reported using the sagittae, whereas it is likely they were actually using the lapilli. This confusion may have resulted because in catfishes (ostariophyseans) the lapilli are the largest of the three otoliths, whereas in nonostariophysean fish the sagittae are the largest. Based on (1) scanning electron microscope microphotographs of channel catfish otoliths, (2) X-ray computed tomography scans of a channel catfish head, (3) descriptions of techniques used to removed otoliths from channel catfish reported in the literature, and (4) a sample of channel catfish otoliths received from fisheries biologists from around the country, it is clear that lapilli are most often used for channel catfish aging studies, not sagittae, as has been previously reported. Fisheries scientists who obtain otoliths from channel catfish can use the information in this paper to correctly identify otolith age.

  9. Population characteristics of channel catfish near the northern edge of their distribution: implications for management

    Science.gov (United States)

    Carter-Lynn, K. P.; Quist, Michael C.

    2015-01-01

    Channel catfish, Ictalurus punctatus (Rafinesque), populations in six lakes in northern Idaho, USA, were sampled to describe their population characteristics. During the summers of 2011 and 2012, 4864 channel catfish were sampled. Channel catfish populations had low to moderate catch rates, and length structure was dominated by fish Channel catfish were in good body condition. All populations were maintained by stocking age-1 or age-2 fish. Growth of fish reared in thermally enriched environments prior to stocking was fast compared to other North American channel catfish populations. After stocking, growth of channel catfish declined rapidly. Once stocked, cold water temperatures, prey resources and (or) genetic capabilities limited growth. Total annual mortality of age 2 and older channel catfish was generally channel catfish population dynamics and highlights important considerations associated with their ecology and management.

  10. Cost and returns analysis of catfish marketing in Aba South Local ...

    African Journals Online (AJOL)

    Cost and returns analysis of catfish marketing in Aba South Local Government Area of Abia State, Nigeria. ... The constraints militating against catfish marketing were also identified. ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  11. Evaluation of portable near infrared spectrophotometer to stage maturity in channel catfish

    Science.gov (United States)

    Gonadal maturity of channel catfish varies within the same cohort of fish. Female channel catfish with superior maturity need to be identified and staged for higher success to induce spawn wit ovulating hormones to produce channel x blue hybrid catfish fry in hatcheries. Maturation is not synchron...

  12. Pyrethroid toxicity in silver catfish, Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    Francisco P. Montanha

    2012-12-01

    Full Text Available This study aimed to determine both the lethal and sublethal concentrations of Cypermethrin in young Silver Catfish (Brazilian "Jundiá", Rhamdia quelen on aquatic environment during 96 hours, as well as to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development period of Rhamdia quelen, and to verify their respective rates of fertilization, hatching and survival. Pyrethroid nowadays is a widely used insecticide, which presents a high toxicity to fish. In order to determine lethal and sublethal concentrations, 120 silver catfish were used; each one had an average weight of 59.58±4.50g and an average size of 20.33±2.34cm. Concentrations used were 0, 1.0, 1.5, 2.0, 2.5, 3.0, 5.0, 10.0, 15.0 and 20.0mg of Cypermethrin per liter of water (mg/L. Fish were exposed to the product in 30-liter fish tanks. In each fish tank there were four fishes and the product was applied three times, i.e., a total of twelve fish were exposed to the product at each application, and a total of 120 fish during the entire experiment (n=120. In order to determine the Cypermethrin and Deltamethrin sublethal concentrations during the initial embryonic development, ovulation induction was performed on female fishes using hormones, and then and egg collection was performed. The eggs were then hydrated and fertilized in Cypermethrin and Deltamethrin in different concentrations: 0.001, 0.01, 0.1, 1.0 and 10.0mg/L of Cypermethrin and 0.001, 0.01, 0.1, 0.5 and 1.0mg/L of Deltamethrin, in addition to the control group (0mg/L. After fertilization, the eggs were kept in containers with the respective pesticides of Cypermethrin and Deltamethrin until hatching, when hatching rate was verified. Then the alevins, from the hatching, were kept on their respective concentrations of Cypermethrin and Deltamethrin so that the survival rate could be analyzed regarding the tested insecticides, during both 12-hour and 24-hour periods

  13. PERFORMA PERTUMBUHAN, KOEFISIEN VARIASI, DAN HETEROSIS HASIL PERSILANGAN IKAN PATIN (Pangasius sp. PADA TAHAP PENDEDERAN II

    Directory of Open Access Journals (Sweden)

    Jadmiko Darmawan

    2017-05-01

    Full Text Available Hibridisasi merupakan salah satu teknik pemuliaan ikan dalam rangka mendapatkan varietas unggul sehingga mampu meningkatkan nilai produksi suatu komoditas ikan yang dibudidayakan. Tujuan penelitian ini adalah untuk mengevaluasi performa pertumbuhan, koefisien variasi, dan nilai heterosis dari hasil persilangan tiga spesies ikan patin sebagai tetua, yaitu patin siam (Pangasianodon hypophthalmus, patin jambal (Pangasius djambal, dan patin nasutus (Pangasius nasutus pada tahap pendederan II. Induk ikan patin siam dan patin jambal yang digunakan merupakan ikan yang sudah dirilis sebagai ikan budidaya, sedangkan induk ikan patin nasutus berasal dari perairan umum dan sedang dalam proses domestikasi sebagai ikan budidaya. Persilangan yang dibuat adalah: A f  patin jambal >0,05. Ikan patin SN memiliki performa yang lebih baik dari tetuanya dengan nilai heterosis bobot akhir, LPS bobot, panjang total, LPS panjang total, dan sintasan berturut-turut sebesar 110,87%; 19,78%; 36,14%, 36,09%; dan 15,04%; serta nilai koefisien variasi berkisar antara 0,00-11,08. Bobot akhir, panjang total dan sintasan ikan patin SJ juga lebih baik dari tetuanya dengan nilai heterosis berturut-turut sebesar 46,00%; 11,27%; dan 2,27%; namun untuk heterosis LPS bobot dan LPS panjang total bernilai negatif (-6,65% dan -1,01%, serta nilai koefisien variasi berkisar antara 0,00-12,75. Berdasarkan hasil penelitian tersebut menunjukkan bahwa ikan patin hibrida SN berpotensi sebagai ikan budidaya dalam rangka peningkatan produksi ikan patin daging putih selain dari ikan patin hibrida  SJ (pasupati yang telah dirilis ke masyarakat. Hybridization is a fish breeding technique in order to obtain high yielding varieties as to increase the production value of a farmed fish. The purpose of this study was to evaluate the growth performance, the coefficient of variation and heterosis value of the result of crossing three species of catfish as a parent, which was striped catfish

  14. THE ADDED VALUE OF SHREDDED LELE AND PATIN CATFISH

    Directory of Open Access Journals (Sweden)

    Ristina Siti Sundari

    2017-09-01

    Full Text Available Fish is not only perishable product but also has segmented market. Consumption market such wants the fresh fish and certainty size. The problem is when the size of fish is too big for consumption, so that product is not wanted by consumer anymore. This research aimed at knowing the added value of shredded Lele and Patin catfish agribusiness and increasing prosperity of humanitarian society throughout processing the shredded catfishes. The data of this research was analyzed by Added Value Analysis of Hayami. The result showed that the shredded product of Lele catfish gave the added value IDR 14.295,00 per kilograms with the added value ratio was 25,53 percent and Conversion value was 0,35. Whereas, the shredded product of Patin catfish gave the added value IDR 18.295,00 per kilogram with the added value ratio was 29,04 percent and Conversion value was 0,35. The agribusiness toward processing and marketing of shredded Lele and Patin catfish was innovative agribusiness that could develop business opportunity so that it could move on the economical wheel and increasing humanitarian society prosperity actively. The market demand was still very wide either in town or out of town. The partnership among various not only government but also non government associations would be a good matter toward this agribusiness is running well.

  15. Stress in African catfish (Clarias gariepinus) following overland transportation.

    Science.gov (United States)

    Manuel, Remy; Boerrigter, Jeroen; Roques, Jonathan; van der Heul, Jan; van den Bos, Ruud; Flik, Gert; van de Vis, Hans

    2014-02-01

    Of the many stressors in aquaculture, transportation of fish has remained poorly studied. The objective of this study was therefore to assess the effects of a (simulated) commercial transportation on stress physiology of market-size African catfish (Clarias gariepinus). Catfish weighing approximately 1.25 kg were returned to the farm after 3 h of truck-transportation, and stress-related parameters were measured for up to 72 h following return. Recovery from transportation was assessed through blood samples measuring plasma cortisol, glucose and non-esterified fatty acids (NEFA) and gill histology. Also, the number of skin lesions was compared before and after transport. Pre-transport handling and sorting elevated plasma cortisol levels compared to unhandled animals (before fasting). Plasma cortisol levels were further increased due to transportation. In control fish, plasma cortisol levels returned to baseline values within 6 h, whereas it took 48 h to reach baseline values in transported catfish. Plasma glucose and NEFA levels remained stable and were similar across all groups. Transported catfish did not, on average, have more skin lesions than the handling group, but the number of skin lesions had increased compared to unhandled animals. The macroscopic condition of the gills was similar in control, transported and unhandled catfish; however, light microscopy and immunohistochemistry revealed atypical morphology and chloride cell migration normally associated with adverse water conditions. From our data, we conclude that transportation may be considered a strong stressor to catfish that may add to other stressors and thus inflict upon the welfare of the fish.

  16. Effects of lead, molybdenum, rubidium, arsenic and organochlorines on spermatogenesis in fish: Monitoring at Mekong Delta area and in vitro experiment

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, Sonoko [Laboratory of Fish Reproductive Physiology, Ehime University, Matsuyama, Ehime 790-8566 (Japan); Miura, Chiemi [Laboratory of Fish Reproductive Physiology, Ehime University, Matsuyama, Ehime 790-8566 (Japan); Ito, Aki [Laboratory of Fish Reproductive Physiology, Ehime University, Matsuyama, Ehime 790-8566 (Japan); Agusa, Tetsuro [Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577 (Japan); Iwata, Hisato [Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577 (Japan); Tanabe, Shinsuke [Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577 (Japan); Bui Cach Tuyen [Nong Lam University, Thu Duc Distr., Ho Chi Minh City (Viet Nam); Miura, Takeshi [Laboratory of Fish Reproductive Physiology, Ehime University, Matsuyama, Ehime 790-8566 (Japan)]. E-mail: miutake@agr.ehime-u.ac.jp

    2007-06-05

    To estimate the influence of water contaminants on fish reproduction in the Mekong Delta area, we sampled cultivated male catfish (Pangasianodon hypophthalmus), investigated testicular development, and measured persistent organic pollutants (POPs) and trace element levels in muscle and liver, respectively. Various testes sizes were observed although sampling took place during a short period. Histological analysis revealed that all developmental stages of germ cells were observed in catfish with large testis, whereas only necrotic spermatogonia but no other germ cells were observed in catfish with small testis. In small testis, furthermore, vacuolization and hypertrophy of Sertoli cells were observed. Measurement of POPs in muscle and trace elements in liver demonstrated that there were negative correlations between GSI and the concentrations of Pb, Mo, Rb and As. To clarify possible direct effects of Pb, Mo, Rb and As on spermatogenesis in fish, we investigated the effects of these trace elements on spermatogenesis using in vitro testicular organ culture of Japanese eel (Anguilla japonica). Treatment with each of the trace elements alone did not affect spermatogenesis. However, treatment with 10{sup -7} M of Pb, 10{sup -5} and 10{sup -4} M of Mo, 10{sup -5}-10{sup -3} M of Rb or 10{sup -5} M of As inhibited the spermatogenesis induced by 11-ketotestosterone (11KT). Furthermore, treatment with 10{sup -4} M of As in combination with 11KT caused necrosis of testicular fragments. Taken together, these results are consistent with the hypothesis that Pb, Mo, Rb and As can exert inhibitory effects on spermatogenesis in catfish inhabiting the Mekong Delta area.

  17. Perceived versus real toxicological safety of pangasius catfish: a review modifying market perspectives

    NARCIS (Netherlands)

    Murk, Albertinka J.; Rietjens, I.M.C.M.; Bush, S.R.

    2018-01-01

    Mass media reports suggest that pangasius (Pangasianodon hypophthalmus) is ‘full of poison’ because it is able to survive in the ‘heavily polluted Mekong River’ and contains pesticides and veterinary treatment chemicals. However, most of these claims are not substantiated with scientific evidence.

  18. Designing Customer Education Activities to Change People’s Perception of Catfish

    Directory of Open Access Journals (Sweden)

    Stevanus Pratama Suwito

    2017-06-01

    Full Text Available Most people see catfish as an unhygienic fish, so less people want to consume it. In fact, catfish has better nutrition compared to Gourami. Therefore, catfish producers need to educate people. The purpose of this research is to design customer education activities to change people’s perception towards catfish using marketing communication mix. This research is a qualitative research and it uses in-depth interview method in gathering the required data. Based on the preliminary survey conducted to 50 people in Surabaya, it is known that Surabaya people are considered novice customers. As such, catfish producers can conduct customer education activities until people want to taste the catfish. The company can add picture, video, and oral information about the benefits in consuming catfish or the nutrition and the certificate of hygienic cultivation system. The producers can provide tester for the catfish and show the customers the comparison between good and bad quality catfish. The producers can also add two more marketing communication activities i.e. events and experiences as well as public relations and publicity. The producers need to use reliable sources in informing the catfish’s nutrition facts such as the certificate of catfish breeder, and certification from BPOM.

  19. Dissolved oxygen and aeration in ictalurid catfish aquaculture

    Science.gov (United States)

    Feed-based production of ictalurid catfish in ponds is the largest aquaculture sector in the United States. The feed biochemical oxygen demand (FBOD) typically is 1.1-1.2 kg O2/kg feed. Feed also results in a substantial increase of carbon dioxide, ammonia nitrogen, and phosphate to ponds, and this ...

  20. Effect of Rearing Periods on the Production of Hybrid Catfish ...

    African Journals Online (AJOL)

    Fingerling production of hybrid catfish (Clarias gariepinus x Heterobanchus longifilis) was investigated over three rearing periods of 3 weeks, 4 weeks and 5 weeks in fertilized earthen ponds during the rainy season. At harvest, fingerling mean weight was directly related to length of rearing period, while survival rate and ...

  1. Culture of sharptooth catfish, Clarias gariepinus in southern Africa

    CSIR Research Space (South Africa)

    Hecht, T

    1988-01-01

    Full Text Available This report presents the proceedings of a two-day workshop held on the sharptooth catfish farm “Moirah” of Mr W Uys in the Eastern Transvaal in late January 1988. The overall aim of the workshop was to synthesize the available information...

  2. Hepatic pathologies in the brackish water catfish ( Chrysicthys ...

    African Journals Online (AJOL)

    Hepatic histopathology recorded in livers from feral populations of the brackish water catfish Chrysichthys nigrodigitatus from locations on the Lagos lagoon complex with significant anthropogenic inputs from denizen populations and industries are presented. Liver sections from sixty specimens from two locations on the ...

  3. Welfare of African catfish : effects of stocking density

    NARCIS (Netherlands)

    Nieuwegiessen, van de P.G.

    2009-01-01

    The general aim of this thesis was to determine the impact of stocking density on welfare indicators in African catfish, Clarias gariepinus, cultured in a recirculating aquaculture system. The following factors were studied: 1) the effects of stocking density on physical, physiological, and

  4. Food and feeding habits of the catfish, Bagrus docmak (Forsskal ...

    African Journals Online (AJOL)

    The natural food of the catfish Bagrus docmak was studied from February 1995 to February 1996 in Lake Chamo, Ethiopia. The stomach contents of a total of 534 fish samples, ranging in size from 33—99 cm fork length (LF) and 500—17400 g in total weight (WT) were investigated. The diet of B. docmak was composed of ...

  5. Estimates of age, growth and mortality of spotted catfish, Arius ...

    African Journals Online (AJOL)

    Administrator

    2011-11-02

    Nov 2, 2011 ... the major by-catch species, and hence more attention should be paid to the dynamics of this stock. .... reflect the sea surface temperature of fishing ground (in this study, ... growth curve of spotted catfish is shown in Figure 4.

  6. Semen collection and preservation in African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Viveiros, A.

    2002-01-01

    Stock improvement using quantitative and molecular genetics is an essential part of nowadays production of farm animals and fish. To achieve this in aquaculture, germplasm of both parental sexes should be obtained in a life-saving manner. In captivity, male African catfish,

  7. Study on Feeding Habit of Clariid Catfish ( Clarias Gariepinus ...

    African Journals Online (AJOL)

    A survey was conducted to investigate the feeding habit of catfish (Clarias gariepinus Burchell, 1822) in Otamiri River, South-Eastern Nigeria. Stomach items analyzed include mainly algae, fish scales, annelids, benthic invertebrates, and detritus confirmed the fish as omnivorous species. However, few stomach contents ...

  8. Behavioural and biochemical responses of juvenile catfish ( Clarias ...

    African Journals Online (AJOL)

    The behavioural and serum liver enzyme responses of juvenile catfish (Clarias gariepinus) were evaluated for 72 hours. Thirty-six (36) healthy fishes with standard weight, 20 ± 1.52 g and standard length, 18.25 ± 0.50 cm were used for the experiment in non-renewable bioassay system. The test fish exhibited stressful ...

  9. Lip histology of the adult farmed African Catfish ( Clarias gariepinus ...

    African Journals Online (AJOL)

    The lip histology of the adult farmed African catfish was investigated to fill the knowledge gap in available literature especially those from commercial concrete tanks. This microanatomy is important as the lip is the principal organ involved in prehension. The lip epidermis was lined by stratified squamous epithelium ...

  10. Comparative study on the growth performance of the hybrid catfish ...

    African Journals Online (AJOL)

    Growth performance of the hybrid catfish Heteroclarias reared in concrete and earthen pond systems were investigated in a 92-day experiment. Experiment was conducted using four rectangular ponds (2 concrete and 2 earthen) each measuring 14 × 6 × 1.5 metres in duplicates. The ponds were uniformly limed, fertilized ...

  11. Anaemia in the freshwater catfish Clarias albopunctatus (Teleostei ...

    African Journals Online (AJOL)

    Haematological changes were studied in freshwater catfish Clarias albopunctatus exposed for 20 days to 25%, 50% and 100% concentrations of brewery wastewater prepared by dilution using tap water. Haemoglobin, haematocrit and erythrocyte counts in fish exposed to wastewater were significantly lower than in a ...

  12. Assessment of the nutritional quality of smoked catfish ( Clarias ...

    African Journals Online (AJOL)

    This study examined the nutritional quality of smoked Catfish Clarias gariepinus obtained in three different markets in Lapai, Niger State, Nigeria for a period of three months. Proximate compositions of smoked Clarias gariepinus were determined. The highest protein content (49.27 ± 2.59) was recorded in smoked fish ...

  13. Performance of Catfish ( Clarias Gariepinus Burchell, 1822) Fed ...

    African Journals Online (AJOL)

    The effect of boiled mango seed kernel (MSK) as substitute for maize in diets of catfish (Clarias gariepinus) fingerlings was investigated using four diets were formulated such that T2 , T3 and T4 contained boiled MSK as replacement for maize at 20%, 40% and 60% inclusion levels respectively, while T1 (control) contained ...

  14. Occurrence and vulnerability of a giant sea catfish, Arius thalassinus ...

    African Journals Online (AJOL)

    The distribution and some biological characteristics of commercially important giant sea catfish, Arius thalassinus (Rüppell, 1837) were studied in Mafia Island from October 2010 to October 2011. Samples were collected from artisanal fishing gear, mainly longlines, sharknets and ringnets, which yielded a total of 2,723 kg ...

  15. Nasal cavity of the farmed african catfish Clarias gariepinus ...

    African Journals Online (AJOL)

    The nasal cavity microanatomy of farmed fingerlings of African catfish, Clarias gariepunus was investigated to fill existing knowledge gap on its histology from available literatures. This becomes very important as no gustatory cell was reported from previous researches on the fingerling digestive tract. A slice of nasal cavity of ...

  16. Formulation, production and evaluation of floating catfish feed ...

    African Journals Online (AJOL)

    This trial was carried out to compare the performance of catfish fed with four experimental diets; a reference foreign diet (Multifeed) and three isonitrogenous and isoenergetic locally formulated diets with different palm oil contents (1, 2 and 3 liters) coded as Feed 1, Feed 2, Feed 3 and Feed 4 respectively. The growth trial ...

  17. Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron

    Science.gov (United States)

    Originally reported in 1983, channel catfish anemia (CCA), also ‘white lip’ or ‘no blood,’ is a major idiopathic disease affecting commercial production in the Mississippi Delta region of the USA. Affected individuals are characterized by lethargy, anorexia, extreme pallor, and packed cell volumes o...

  18. 76 FR 26655 - Public Meetings on the Proposed Rule for Mandatory Inspection of Catfish and Catfish Products

    Science.gov (United States)

    2011-05-09

    ... for catfish under provisions of the Agricultural Marketing Act (7 U.S.C. 1622, 1624) and regulations..., large print, audiotape, etc.) should contact USDA's Target Center at (202) 720-2600 (voice and TTY). To... the Web site, FSIS is able to provide information to a much broader, more diverse audience. In...

  19. PRELIMINARY STUDY OF FISH CULTURE IN ABANDONED SAND MINING POOL

    Directory of Open Access Journals (Sweden)

    Bambang Gunadi

    2006-12-01

    Full Text Available One of main problems in freshwater aquaculture development in Indonesia, especially in Java, is unavailability of developing zone. It is important to find an underutilized area that meets for industrial scale freshwater aquaculture, i.e. sufficient water supply, wide area, and located in one area or zone. The abandoned mining (sand, tin, etc. pools distributed along the country might be the potential area for freshwater aquaculture business. For example, there are at least 13 water pools with total surface area of 250 ha at 15 km side of Citarum River in Karawang District (West Java Province. This study was conducted to obtain preliminary data about the prospect and potency of fish culture (tilapia, clariid catfish, and ‘patin’ catfish in abandoned sand-mining pools in Karawang District. Mini floating net cages of 1 x 1 x 1.5 m3 size were used for culturing fish, i.e. patin catfish (Pangasianodon hypophthalmus, nile tilapia (Oreochromis niloticus, and clariid catfish (Clarias gariepinus, separately. Patin catfish were stocked at a size of 2 g with a density of 300 fish per cage, tilapia were stocked at a size of 6 g with a density of 400 fish per cage, while  the clariid catfish were stocked at a size of 1.4 g with a density of 980 fish per cage. A floating commercial feed (30%—32% protein, 3%—5% fat was used at a daily rate of 9% biomass weight at the beginning and reduced gradually to 3% at the final culture period. Observed data showed that patin catfish grew from the initial size of 2.08 g to the final size 299.59 g in 5 months, nile tilapia grew from individual initial size of 5.92 g to the final size of 247.12 g in 14 weeks, and clariid catfish grew from initial size of 1.39 g to the final size of 73.10 g in 8 weeks. These three species were technically prospective for aquaculture development in the abandoned sand-mining pools.

  20. INTRA-PROCESSOR PRICE-SPREAD BEHAVIOR: IS THE U.S. CATFISH PROCESSING INDUSTRY COMPETITIVE?

    OpenAIRE

    Hudson, Darren

    1998-01-01

    An analysis was conducted of price-spread behavior in the catfish-processing sector of the United States. A model of imperfect competition using conjectural variations was used to test for significant deviations from competition. Results show no significant deviation from competitive behavior, suggesting that catfish processor behave competitively. However, this result is limited by the assumption of equal market shares by each catfish-processing firm.

  1. Protein digestibility and ammonia excretion in catfish Clarias gariepinus culture

    Directory of Open Access Journals (Sweden)

    Bambang Gunadi

    2014-01-01

    Full Text Available ABSTRACT A series of experiments was performed to analyze protein digestibility, ammonia excretion, and also heterothropic bacteria and phytoplankton dynamics in the catfish Clarias gariepinus culture. In the digestibility experiment, catfish with an individual initial size of 43.67±0.83 g were stocked into 120 L conical fiberglass tanks at a density of 20 fish per tank. Fish were fed on with commercial diet supplemented with Cr2O3 indicator at a concentration of 1%. In the ammonia excretion experiment, catfish with an individual size of 111.6±9.5 and 40.6±3.4 g, respectively,  were placed into a 10 L chamber filled with 8 L of water. Total ammonia nitrogen (TAN in the chambers were monitored every hour for six consecutive hours. In the bacteria and phytoplankton dynamics experiment, catfish were stocked in the 25 m2 concrete tanks which was divided into two compartments (catfish 10 m2, and heterotrof compartments 15 m2. Catfish with individual size of 42,5±0 g were stocked into the tanks at a density of 100 fish per tank. Water was recirculated from catfish compartments to heterotrophic compartments. Fish were fed with floating feed. Molasses as carbon source for heterotrophic bacteria was applied daily. The experiment was conducted for six weeks. The results showed that the protein digestibility was 61.97±7.24%. Larger fish (size of 111.6 g excreted ammonia at a rate of 0.008±0.003 mg TAN/g fish-weight/hour, which was lower than that of the smaller catfish (size of 40.6 g, i.e. 0.012±0.004 mg TAN/g fish-weight/hour. Keywords: protein digestibility, ammonia excretion, catfish  ABSTRAK Serangkaian penelitian telah dilakukan untuk menganalisis ketercernaan pakan dan protein, ekskresi amonia, serta dinamika bakteri dan fitoplankton pada budidaya ikan lele (Clarias gariepinus. Pada penelitian ketercernaan pakan, ikan lele berukuran 43,67±0,83 g/ekor dipelihara dalam bak fiberglas berbentuk corong berukuran 120 L dengan kepadatan 20

  2. Channel catfish response to ultraviolet-B radiation

    Science.gov (United States)

    Ewing, M.S.; Blazer, V.S.; Fabacher, D.L.; Little, E.E.; Kocan, K.M.

    1999-01-01

    Fingerling channel catfish Ictalurus punctatus exposed to simulated ultraviolet-B radiation at an average daily dose of 2.9 J/cm2 were quite sensitive to the radiation. After a 24-h exposure, thinning of the most dorsal epidermis frequently was accompanied by edema. Compared with epidermis of unexposed fish, mucous cells in exposed fish were less superficial and club cells were less numerous both dorsally and high on the lateral surface of the body. Sunburn cells with pyknotic nuclei were evident in the epidermis of exposed fish. Among fish exposed for 48 h, focal necrosis and sloughing of the outer epidermal layer were widespread. A methanol-extractable skin substance that is associated with resistance to sunburn in other fish species was not detected in channel catfish.

  3. Energy costs of catfish space use as determined by biotelemetry.

    Directory of Open Access Journals (Sweden)

    Ondřej Slavík

    Full Text Available Animals use dispersed resources within their home range (HR during regular day-to-day activities. The high-quality area intensively used by an individual, where critical resources are concentrated, has been designated as the core area (CA. This study aimed to describe how animals utilize energy in the HR and CA assuming that changes would occur according to the size of the used areas. We observed energetic costs of space use in the largest European freshwater predator catfish, Silurus glanis, using physiological sensors. Catfish consumed significantly more energy within the CA compared to the rest of the HR area. In addition, energetic costs of space use within a large area were lower. These results generally indicate that utilization of larger areas is related to less demanding activities, such as patrolling and searching for new resources and mates. In contrast, fish occurrence in small areas appears to be related to energetically demanding use of spatially limited resources.

  4. KOEFISIEN KECERNAAN FRAKSI SERAT BUNGKIL KELAPA SAWIT YANG DIHIDROLISIS DENGAN ENZIM ASAL CAIRAN RUMEN DOMBA SEBAGAI PAKAN BENIH IKAN PATIN SIAM (Pangasius hypophthalmus

    Directory of Open Access Journals (Sweden)

    Wahyu Pamungkas

    2012-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui nilai kecernaan fraksi serat bungkil kelapa sawit yang dihidrolisis dengan enzim cairan rumen domba. Koefisien kecernaan ditentukan dengan menggunakan indikator Cr2O3 yang ditambahkan dalam pakan uji. Bahan pakan yang digunakan adalah bungkil kelapa sawit (BKS yang dihidrolisis dengan enzim asal cairan rumen domba dengan volume 100 mL/kg BKS dan diinkubasi selama 24 jam (BKSe dan yang tidak dihidrolisis (BKS. Pakan yang digunakan dalam penelitian adalah pakan acuan (pakan komersil, pakan uji A (30% BKSe dan B (30% BKS dengan 3 ulangan. Ikan yang digunakan 10 ekor benih ikan patin siam Pangasius hypophthalmus dengan bobot rata-rata 20 g/ekor yang dipelihara dalam fiber dengan volume air 80 liter. Pemberian pakan secara at satiation dengan frekuensi pemberian 3 kali per hari. Feses dikumpulkan selama 15 hari pemeliharaan untuk dianalisis kandungan nutrisinya. Hasil analisis kecernaan menunjukkan bahwa nilai kecernaan fraksi serat menunjukkan adanya perbedaan yang nyata (P<0,05 antar perlakuan di mana pakan A (30% BKSe mempunyai nilai kecernaan fraksi serat lebih tinggi dibandingkan pakan B (30% BKS.

  5. Bioaccumulation of P-32 in bluegill and catfish

    International Nuclear Information System (INIS)

    Kahn, B.; Turgeon, K.S.; Martini, D.K.; Dunkerly, S.J.; El-Shinawy, R.M.K.; Wilson, M.D.; Hammond, R.A.; Uribe, R.; Mizner, A.A.

    1985-02-01

    Bluegill and catfish were fed P-32 at a constant feeding rate per body weight to determine the bioaccummulation factor (BF/sub r/) for P-32 in muscle relative to water. The fish were maintained in flow-through tanks at two feeding levels. The bluegill accumulated P-32 for 51 days, followed by depuration for 28 days. The catfish study had to be teminated after 11 days. Fish were analyzed in triplicte for P-32 and phosphorus at intervals of 1 to 8 days. Additional aquaria experiments were performed to determine the effects of water temperature, feeding rate, and type of food (worms vs. pellets) on P-32 uptake, and to observe P-32 uptake from water by unfed fish (including fish with blocked esophagus). A simple calculational model was used to determine the phosphorus turnover constant from the specific activity in tissue relative to food. This ratio at steady state approaches the BF/sub r/BF ratio (where BF is the phosphorus bioaccumulation factor) if P-32 transfers rapidly from water to food. The bluegill showed a weight gain of 0.2 %/d, a phosphorous turnover constant in muscle of 0.43 %/d, and a BF/sub r//BF ratio of 0.081 at the higher feeding rate, and 0.05 %/d, 0.34 %/d, and 0.064 at the lower feeding rate. Hence, respective P-32 BF/sub r/ values are 6000 and 4000 at a phosphorus BF of 70,000. The BF/sub r/ values for catfish were approximately twice as high. The aquarium experiments suggest that the higher factors are due to a much higher phosphorus intake, higher water temperature, higher retention from pellets than from worms, and possible higher retention by catfish than bluegill under the same conditions. 36 references, 15 figures, 22 tables

  6. Cost Characteristics of the African Catfish Culture in Recirculating ...

    African Journals Online (AJOL)

    Cost Characteristics of the African Catfish Culture in Recirculating Production Facilities in Ibadan, Oyo State, Nigeria. ... Food Conversion Ratio also ranged from 0.60–0.78 for fingerlings, 1.01-‐ 2.08 for juveniles and 1.15-‐1.68 for grow out system. In all cases, variable cost accounts for more than 75% of total cost.

  7. Histopathological changes in the Brain Tissue of Africa Catfish ...

    African Journals Online (AJOL)

    One hundred and twenty normal post juvenile catfish (Clarias gariepinus) of both sexes with a mean weight of 135.44±1.99 g and mean length of 28.32±0.844 cm were purchased from Osayi farm. They were kept in 60 l aquaria at 27.5±0.4 ◦C, pH 7.3, with 12:12 h photoperiod, well aerated, provided with external filtration ...

  8. Pectoral sound generation in the blue catfish Ictalurus furcatus.

    Science.gov (United States)

    Mohajer, Yasha; Ghahramani, Zachary; Fine, Michael L

    2015-03-01

    Catfishes produce pectoral stridulatory sounds by "jerk" movements that rub ridges on the dorsal process against the cleithrum. We recorded sound synchronized with high-speed video to investigate the hypothesis that blue catfish Ictalurus furcatus produce sounds by a slip-stick mechanism, previously described only in invertebrates. Blue catfish produce a variably paced series of sound pulses during abduction sweeps (pulsers) although some individuals (sliders) form longer duration sound units (slides) interspersed with pulses. Typical pulser sounds are evoked by short 1-2 ms movements with a rotation of 2°-3°. Jerks excite sounds that increase in amplitude after motion stops, suggesting constructive interference, which decays before the next jerk. Longer contact of the ridges produces a more steady-state sound in slides. Pulse pattern during stridulation is determined by pauses without movement: the spine moves during about 14 % of the abduction sweep in pulsers (~45 % in sliders) although movement appears continuous to the human eye. Spine rotation parameters do not predict pulse amplitude, but amplitude correlates with pause duration suggesting that force between the dorsal process and cleithrum increases with longer pauses. Sound production, stimulated by a series of rapid movements that set the pectoral girdle into resonance, is caused by a slip-stick mechanism.

  9. Celioscopic liver biopsy in silver catfish (Rhamdia quelen

    Directory of Open Access Journals (Sweden)

    João P.S. Feranti

    2015-01-01

    Full Text Available Endosurgery has been used for assessment of fish celomatic cavity, as well as for obtaining biopsies for organic analysis. Such minimally invasive access may also be used for the analysis of environmental impact on biomarkers of pollution. In Brazil, studies and literature regarding the use of celioscopy in fish are sparse. The purpose of the current study was to develop a two-port celioscopy technique to obtain liver biopsy in silver catfish (Rhamdia quelen. Six adult female silver catfish were used. The animals were anesthetized and the inspection of the celomatic cavity were performed using a telescope and celioscopic-guided liver biopsy were taken using laparoscopic Kelly forceps. On the early postoperative period, the animals were released in a confined water reservoir where mortality could be checked. The liver samples were sent for histological assessment. There were no complications during surgery on early postoperative period. It was possible to visualize meticulously several organs (liver, spleen, stomach, pancreas, swim bladder, ovaries, bowel and transverse septum. In conclusion, the surgical technique and the anesthetic protocol proposed were suitable to perform liver biopsies in silver catfish and provided low morbidity.

  10. African catfish (Clarias gariepinus Burchell, 1822. Thematic bibliography

    Directory of Open Access Journals (Sweden)

    I. Hrytsyniak

    2017-12-01

    Full Text Available Purpose. Forming a thematic bibliographic list of publications on certain issues of African catfish (Clarias gariepinus biology and cultivation in Ukraine and abroad, as well as concerning the effects of rearing conditions on physiological, biochemical and fish-breeding characteristics of clariids. Methods. The complete and selective methods were applied in the process of the systematic search. The bibliographic core have been formed with the publications in Ukrainian, Russian and English from the fund of scientific library of the Institute of Fisheries NAAS of Ukraine. Results. There was composed the thematic list of publications with a total quantity of 112 sources covering the time interval from 1978 to 2017, as well as an article from the "Aquaculture" journal, dated 2018, and including the fish-biological characteristics of African catfish as representative of Siluriformes order, Clariidae family. The literary sources are arranged in alphabetical order by author or title, and described according to DSTU 8302:2015 “ Information and documentation. Bibliographic reference. General principles and rules of composition”, with the amendments (code UKND 01.140.40, as well as in accordance with the requirements of APA style — international standard of references. Practical value. The list may be useful for scientists, practitioners, students, whose area of interests covers the questions of cultivation and study of the biological features of African catfish.

  11. Remediation of uranium-contaminated groundwater by sorption onto hydoxyapatite derived from catfish bones

    Science.gov (United States)

    Hydroxyapatite was prepared from catfish bones, called catfish hydroxyapatite (CFHA), by mechanical and chemical treatment methods and was characterized by x-ray diffraction (X-RD) and scanning electron microscope (SEM) techniques to confirm the presence of hydroxyapatite. The ability of CFHA to rem...

  12. Performance evaluation of intensive, pond-based culture systems for catfish production in Mississippi: year one

    Science.gov (United States)

    Catfish farming is the leading form of aquaculture in the U.S. and has a significant economic impact in the southern states. Increased feed and energy costs, and competition from foreign imports have recently caused catfish farmers to intensify production as a means of improving efficiency and profi...

  13. Destiny-yield relationship for channel catfish reared in a biofloc technology production system

    Science.gov (United States)

    The effect of stocking density on yield of stocker channel catfish and water quality in a biofloc technology production system was studied in this completely randomized design experiment. Fingerling channel catfish (Ictalurus punctatus; 48.0 g/fish, 17.8 cm/fish) were stocked into nine continuously ...

  14. Growth performance, survival, and processing characteristics of hybrid catfish fed pre And probiotics

    Science.gov (United States)

    There has been a lot of interest in the use of pre and probiotics to increase growth and improve disease resistance in the catfish industry. This study aimed to evaluate a commercially available prebiotic and probiotic under conditions simulating commercial production in hybrid catfish. The dietar...

  15. Molecular Cloning and Sequencing of Hemoglobin-Beta Gene of Channel Catfish, Ictalurus Punctatus Rafinesque

    Science.gov (United States)

    : Hemoglobin-y gene of channel catfish , lctalurus punctatus, was cloned and sequenced . Total RNA from head kidneys was isolated, reverse transcribed and amplified . The sequence of the channel catfish hemoglobin-y gene consists of 600 nucleotides . Analysis of the nucleotide sequence reveals one o...

  16. Efficiency of resource-use and elasticity of production among catfish ...

    African Journals Online (AJOL)

    This study employed the use of the Stochastic Frontier Production Function in the empirical analysis of efficiency of resource-use and elasticity of production among catfish farmers in Kaduna, Nigeria. The simple random sampling technique was employed in selecting 60 catfish farmers drawn from the sampling frame ...

  17. Profitability and viability of catfish enterprises in Abia State of Nigeria ...

    African Journals Online (AJOL)

    This study was designed to analyze profitability and viability of catfish farming in Abia State of Nigeria. Simple random sampling technique was used in selecting 50 catfish farmers that provided the data used for the study. The primary data were collected with the aid of structured questionnaire, administered through ...

  18. Effects of Endosulfan on Predator–Prey Interactions Between Catfish and Schistosoma Host Snails

    NARCIS (Netherlands)

    Monde, Concillia; Syampungani, Stephen; Brink, van den Paul J.

    2016-01-01

    The effect of the pesticide endosulfan on predator–prey interactions between catfish and Schistosoma host snails was assessed in static tank experiments. Hybrid catfish (Clarias gariepinus × C. ngamensis) and Bulinus globosus were subjected to various endosulfan concentrations including an

  19. The potential for using red claw crayfish and hybrid African catfish as ...

    African Journals Online (AJOL)

    The potential of red claw crayfish and hybrid African catfish (Clarias gariepinus and Clarias ngamensis) as predators for Schistosoma host snails was evaluated in 2014 by monitoring the consumption of snails by crayfish and catfish in experimental tanks over time under laboratory conditions. After 15 days, both crayfish and ...

  20. Influence of Culture Water Draw-off on Growth of the African Catfish ...

    African Journals Online (AJOL)

    An experiment was carried out over a period of 10 weeks to investigate the influence of culture water draw-‐off on growth and feed utilization of the African catfish. Two similar fish culture tanks (designated A and B) were stocked with juveniles of African catfish at 43 fish per m3. The fish were fed twice daily at 3 % body ...

  1. Effect of Stocking Density on Growth and Survival of African Catfish ...

    African Journals Online (AJOL)

    African catfish (Clarias gariepinus Burchell, 1882) fry were cultured at three different stocking densities in net hapas (submerged volume 0.75 m3) suspended in an outdoor concrete tank of the Aquaculture and Fisheries Department, University of Agriculture, Abeokuta, Nigeria. Catfish fry (mean weight 0.12 ± 0.10 g) were ...

  2. Comparative analysis of catfish BAC end sequences with the zebrafish genome

    Directory of Open Access Journals (Sweden)

    Abernathy Jason

    2009-12-01

    Full Text Available Abstract Background Comparative mapping is a powerful tool to transfer genomic information from sequenced genomes to closely related species for which whole genome sequence data are not yet available. However, such an approach is still very limited in catfish, the most important aquaculture species in the United States. This project was initiated to generate additional BAC end sequences and demonstrate their applications in comparative mapping in catfish. Results We reported the generation of 43,000 BAC end sequences and their applications for comparative genome analysis in catfish. Using these and the additional 20,000 existing BAC end sequences as a resource along with linkage mapping and existing physical map, conserved syntenic regions were identified between the catfish and zebrafish genomes. A total of 10,943 catfish BAC end sequences (17.3% had significant BLAST hits to the zebrafish genome (cutoff value ≤ e-5, of which 3,221 were unique gene hits, providing a platform for comparative mapping based on locations of these genes in catfish and zebrafish. Genetic linkage mapping of microsatellites associated with contigs allowed identification of large conserved genomic segments and construction of super scaffolds. Conclusion BAC end sequences and their associated polymorphic markers are great resources for comparative genome analysis in catfish. Highly conserved chromosomal regions were identified to exist between catfish and zebrafish. However, it appears that the level of conservation at local genomic regions are high while a high level of chromosomal shuffling and rearrangements exist between catfish and zebrafish genomes. Orthologous regions established through comparative analysis should facilitate both structural and functional genome analysis in catfish.

  3. Comparison of Channel Catfish and Blue Catfish Gut Microbiota Assemblages Shows Minimal Effects of Host Genetics on Microbial Structure and Inferred Function

    Directory of Open Access Journals (Sweden)

    Jacob W. Bledsoe

    2018-05-01

    Full Text Available The microbiota of teleost fish has gained a great deal of research attention within the past decade, with experiments suggesting that both host-genetics and environment are strong ecological forces shaping the bacterial assemblages of fish microbiomes. Despite representing great commercial and scientific importance, the catfish within the family Ictaluridae, specifically the blue and channel catfish, have received very little research attention directed toward their gut-associated microbiota using 16S rRNA gene sequencing. Within this study we utilize multiple genetically distinct strains of blue and channel catfish, verified via microsatellite genotyping, to further quantify the role of host-genetics in shaping the bacterial communities in the fish gut, while maintaining environmental and husbandry parameters constant. Comparisons of the gut microbiota among the two catfish species showed no differences in bacterial species richness (observed and Chao1 or overall composition (weighted and unweighted UniFrac and UniFrac distances showed no correlation with host genetic distances (Rst according to Mantel tests. The microbiota of environmental samples (diet and water were found to be significantly more diverse than that of the catfish gut associated samples, suggesting that factors within the host were further regulating the bacterial communities, despite the lack of a clear connection between microbiota composition and host genotype. The catfish gut communities were dominated by the phyla Fusobacteria, Proteobacteria, and Firmicutes; however, differential abundance analysis between the two catfish species using analysis of composition of microbiomes detected two differential genera, Cetobacterium and Clostridium XI. The metagenomic pathway features inferred from our dataset suggests the catfish gut bacterial communities possess pathways beneficial to their host such as those involved in nutrient metabolism and antimicrobial biosynthesis, while

  4. The Usage of Garlic Extract (Allium sativum to Cure Pangasius Fish (Pangasius hypophthalmus Infected by Aeromonas hydrophylla

    Directory of Open Access Journals (Sweden)

    . Muslim

    2009-01-01

    Full Text Available The purpose of this research was to know effectivity of the garlic extract to cure Pangasius catfish that infected by A. hidrophylla bacteria. This research was done on February to April 2007, at Aquaculture Laboratory FP UNSRI. This research used Completely Randomised Design (CRD with five treatments and three replications. The concentration of the garlic extract that used were 0 % (control, 0.2 %, 0.4 %, 0.6 %, 0.8 %. The parameters inspected include SR of fish that infected; SR of fish had been treatment, Relative Percent Survival (RPS, clinical symptom and water quality. The highest survival rate percentage is treatments A4 (0.8 % with average value 66.66 %. The best concentration of garlic extract that can heal the fish until ≥ 50 % (RPS value from totally sample of fish were treatments A4 (0.8 %, A3 (0.6 %, and A2 (0.4 %. The clinical symptom after cure and care as long as fourteen days indicated in control (without garlic extract has been found hard damage bodies organ and the mortality fish effect, but in treatments A1 (0.2 %, A2 (0.4 %, A3 (0.6 %, and A4 (0.8 % recover after submerged with garlic extract. The water quality parameters during experiment in each treatments such as temperature was 27 - 29 oC, pH 6-6.5, Dissolved Oxygen 5.24 - 6.87 mg/l, and Ammonia 0.09 - 0.46 mg/l. Keywords: garlic extract, pangasius fish, A. hydrophylla bacteria   ABSTRAK Tujuan penelitian ini untuk mengetahui efektivitas ekstrak bawang putih untuk mengobati penyakit bakterial pada ikan patin yang disebabkan A. hidrophylla. Penelitian ini dilaksanakan pada bulan Februari - April  2007, di Laboratorium Budidaya Perairan, FP UNSRI. Penelitian ini menggunakan rancangan acak lengkap,  5 perlakuan dan 3 ulangan.  Konsentrasi ekstrak bawang putih yang digunakan adalah  0 % (kontrol, 0,2 %, 0,4 %, 0,6 %, 0,8 %.  Parameter yang diamati meliputi SR ikan yang terinfeksi, SR ikan setelah diberi perlakuan, RPS, gejala klinis serta kualitas air. Persentase SR

  5. DNA barcoding of commercially important catfishes in the Philippines.

    Science.gov (United States)

    Quilang, Jonas P; Yu, Shiny Cathlynne S

    2015-06-01

    Many species of catfish are important resources for human consumption, for sport fishing and for use in aquarium industry. In the Philippines, some species are cultivated and some are caught in the wild for food and a few introduced species have become invasive. In this study, DNA barcoding using the mitochondrial cytochrome c oxidase I (COI) gene was done on commercially and economically important Philippine catfishes. A total of 75 specimens belonging to 11 species and 5 families were DNA barcoded. The genetic distances were computed and Neighbor-Joining (NJ) trees were constructed based on the Kimura 2-Parameter (K2P) method. The average K2P distances within species, genus, family and order were 0.2, 8.2, 12.7 and 21.9%, respectively. COI sequences clustered according to their species designation for 7 of the 11 catfishes. DNA barcoding was not able to discriminate between Arius dispar and A. manillensis and between Pterygoplichthys disjunctivus and P. pardalis. The morphological characters that are used to distinguish between these species do not complement molecular identification through DNA barcoding. DNA barcoding also showed that Clarias batrachus from the Philippines is different from the species found in India and Thailand, which supports earlier suggestions based on morphology that those found in India should be designated as C. magur and those in mainland Southeast Asia as C. aff. batrachus "Indochina". This study has shown that DNA barcoding can be used for species delineation and for tagging some species for further taxonomic investigation, which has implications on proper management and conservation strategies.

  6. Digestible energy of crude glycerol for pacu and silver catfish

    Directory of Open Access Journals (Sweden)

    Rafael Ernesto Balen

    2014-01-01

    Full Text Available The increase in global biodiesel production is originating a glycerol surplus, which has no defined destination. An alternative to overcome this problem is its use as energy source in animal feeding. In Brazil, Pacu (Piaractus mesopotamicus is one of the most farmed native fish species, whereas Silver catfish (Rhamdia quelen is suitable for production in subtropical region. Considering little knowledge about crude glycerol utilization in feeds for Neotropical fish species, it was evaluated the apparent digestibility coefficients (ADCs for energy of crude glycerol for P. mesopotamicus and R. quelen. The digestibility and digestible energy content of crude glycerol can be considered excellent even when compared to energy of common ingredients such as maize and wheat, presenting 0.97 and 0.89 of energy ADCs, and 15.2 and 13.95MJ kg-1 of digestible energy for Pacu and Silver catfish, respectively. In conclusion, crude glycerol is an energetic ingredient with good potential in Brazilian native fish diets.

  7. Plastic debris ingestion by marine catfish: an unexpected fisheries impact.

    Science.gov (United States)

    Possatto, Fernanda E; Barletta, Mário; Costa, Monica F; do Sul, Juliana A Ivar; Dantas, David V

    2011-05-01

    Plastic marine debris is a pervasive type of pollution. River basins and estuaries are a source of plastics pollution for coastal waters and oceans. Estuarine fauna is therefore exposed to chronic plastic pollution. Three important catfish species [Cathorops spixii (N=60), Cathorops agassizii (N=60) and Sciades herzbergii (N=62)] from South Western Atlantic estuaries were investigated in a tropical estuary of the Brazilian Northeast in relation to their accidental ingestion of plastic marine debris. Individuals from all three species had ingested plastics. In C. spixii and C. agassizii, 18% and 33% of individuals had plastic debris in their stomachs, respectively. S. herzbergii showed 18% of individuals were contaminated. All ontogenetic phases (juveniles, sub-adults and adults) were contaminated. Nylon fragments from cables used in fishery activities (subsistence, artisanal and commercial) played a major role in this contamination. These catfish spend their entire life cycles within the estuary and are an important feeding resource for larger, economically important, species. It is not yet possible to quantify the scale and depth of the consequences of this type of pollution. However, plastics are well known threat to living resources in this and other estuaries. Conservation actions will need to from now onto take plastics pollution into consideration. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. cost and returns analysis of catfish marketing in aba south local

    African Journals Online (AJOL)

    The study analyzed the cost and returns of catfish marketing in Aba South ... significantly influenced net income should be considered in policy issues. ... entrepreneur/manager at a profit. .... marketers were well experienced in their business.

  9. The feeding ecology of schilbeid catfishes in river Ase, Niger delta ...

    African Journals Online (AJOL)

    The feeding ecology of schilbeid catfishes in river Ase, Niger delta, Southern Nigeria. ... Open Access DOWNLOAD FULL TEXT ... in feeding intensity were described for Eutropius niloticus and Schilbe mystus which had adequate data.

  10. Draft genome sequences of three Aeromonas hybrophila isolates from catfish and tilapia

    Science.gov (United States)

    Aeromonas hydrophila is a Gram-negative bacteria that is particularly adapted to freshwater environments and can cause severe infections in fish and humans. Here we report the draft genomes of three A. hydrophila catfish and tilapia isolates....

  11. Analysis Of Masamo Catfish Marketing By Analytical Approach At Farmer Group “Sumber Lancar”, Blimbing, Malang City

    OpenAIRE

    Abidin, Zainal; Handayani, Wahyu; Fattah, Mochammad

    2016-01-01

    Masamo as new variety of catfish cultivated by the farmer group "Sumber Lancar" in Blimbing, Malang currently has a lot of demand due to increasing consumers who like to eat fish to meet the need for protein for the body. Increasing of Masamo catfish demand followed by production and marketing efforts. This study wants to know whether the marketing efficient. Therefore, this study uses analytical approach approach in order to identify institutional and channel of Masamo Catfish marketing perf...

  12. A common bottlenose dolphin (Tursiops truncatus) prey handling technique for marine catfish (Ariidae) in the northern Gulf of Mexico.

    Science.gov (United States)

    Ronje, Errol I; Barry, Kevin P; Sinclair, Carrie; Grace, Mark A; Barros, Nélio; Allen, Jason; Balmer, Brian; Panike, Anna; Toms, Christina; Mullin, Keith D; Wells, Randall S

    2017-01-01

    Few accounts describe predator-prey interactions between common bottlenose dolphins (Tursiops truncatus Montagu 1821) and marine catfish (Ariopsis felis Linnaeus 1766, Bagre marinus Mitchill 1815). Over the course of 50,167 sightings of bottlenose dolphin groups in Mississippi Sound and along the Florida coast of the Gulf of Mexico, severed catfish heads were found floating and exhibiting movements at the surface in close proximity to 13 dolphin groups that demonstrated feeding behavior. These observations prompted a multi-disciplinary approach to study the predator-prey relationship between bottlenose dolphins and marine catfish. A review was conducted of bottlenose dolphin visual survey data and dorsal fin photographs from sightings where severed catfish heads were observed. Recovered severed catfish heads were preserved and studied, whole marine catfish were collected and examined, and stranding network pathology reports were reviewed for references to injuries related to fish spines. Photographic identification analysis confirms eight dolphins associated with severed catfish heads were present in three such sightings across an approximately 350 km expanse of coast between the Mississippi Sound and Saint Joseph Bay, FL. An examination of the severed catfish heads indicated interaction with dolphins, and fresh-caught whole hardhead catfish (A. felis) were examined to estimate the presumed total length of the catfish before decapitation. Thirty-eight instances of significant trauma or death in dolphins attributed to ingesting whole marine catfish were documented in stranding records collected from the southeastern United States of America. Bottlenose dolphins typically adhere to a ram-feeding strategy for prey capture followed by whole prey ingestion; however, marine catfish skull morphology may pose a consumption hazard due to rigid spines that can puncture and migrate through soft tissue, prompting a prey handling technique for certain dolphins, facilitating

  13. Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus

    OpenAIRE

    Qorie Astria; Sri Nuryati; Kukuh Nirmala; Alimuddin Alimuddin

    2017-01-01

    ABSTRACT Outbreaks of infectious diseases due to Aeromonas hydrophila in catfish can cause high death rates (80–100%). Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem Musa cavendishii var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections A. hyd...

  14. Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets.

    Science.gov (United States)

    Chen, Bang-Yuan; Pyla, Rajkumar; Kim, Tae-Jo; Silva, Juan L; Jung, Yean-Sung

    2010-08-01

    Catfish skins, intestines, fresh fillets, processing surfaces at different production stages, chiller water and non-food contact surfaces were sampled for Listeria monocytogenes and other Listeria species. Among 315 samples, prevalence of L. monocytogenes, Listeria innocua and a group of Listeria seeligeri-Listeria welshimeri-Listeria ivanovii was 21.6, 13.0 and 29.5%, respectively. No Listeria grayi was detected in this survey. While no L. monocytogenes strains were isolated from catfish skins and intestines, the strains were found with a frequency of 76.7% in chilled fresh catfish fillets and 43.3% in unchilled fillets. L. monocytogenes and Listeria spp. were also detected in fish contact surfaces such as deheading machine, trimming board, chiller water, conveyor belts at different stages, and fillet weighing table. Among L. monocytogenes, 1/2b (47.0%), 3b (16.0%) and 4c (14%) were the predominant serotypes isolated, whereas 4b, 4e, 1/2c and 1/2a were detected at much lower frequencies. Genotype analyses of L. monocytogenes isolates using serotyping, pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC)-PCR revealed that chiller water represented an important contamination source of L. monocytogenes in the chilled catfish fillets of two processing facilities, whereas fillet weighing table significantly contributed to the catfish fillet contamination of the third facility. This study suggests that L. monocytogenes contamination in the processed catfish fillets originates from the processing environment, rather than directly from catfish. Results from this study can aid the catfish industry to develop a plant-specific proper cleaning and sanitation procedure for equipment and the processing environment designed to specifically target L. monocytogenes contamination. Copyright 2010 Elsevier Ltd. All rights reserved.

  15. Output Price Risk, Material Input Price Risk, and Price Margins: Evidence from the US Catfish Industry.

    Directory of Open Access Journals (Sweden)

    David Bouras

    2017-07-01

    Full Text Available Aim/purpose - To develop a conceptual model for analyzing the impact of output price risk and material input price risk on price margins. Design/methodology/approach - To analyze the combined effect of output price risk and material input risk on price margins, we use a series of comparative static analyses, GARCH models, and data ranging from 1990/01 to 2012/12. Findings - The theoretical results indicate that the impact of output price risk and the impact of material input price risk on price margins are ambiguous and, to a great extent, hinge on the correlation between output price and material input price. The empirical results show that whole frozen catfish price risk and live catfish price risk negatively affect the price margin for frozen catfish. The empirical results, however, indicate that the risk of the price of live catfish affects markedly the price margin for frozen whole catfish in contrast to the impact of the risk of the price of frozen whole catfish. Research implications/limitations - The empirical results have significant implications for managerial decision-making especially when crafting strategies for improving price margins. Accordingly, in order to beef up the price margin for frozen whole catfish, catfish processors may consider engaging in vertical integration. This paper has some limitations: first, it assumes that firms operate in competitive markets; second, it assumes that firms produce and sell a single product. Originality/value/contribution - Unlike earlier studies that focused solely on the effect of output price risk on price margins, this paper analyzes theoretically and empirically the impact of output price risk and material input price risk on price margins.

  16. Characteristics Of Smoked Catfish (Hemibagrus Nemurus) Prepared Frompond Culture, Cage And Wild Fish

    OpenAIRE

    Maskilin, Jippo; Hasan, Bustari; Leksono, Tjipto

    2017-01-01

    This study was aimed to evaluate the sensory quality, proximate composition, amino acids and fatty acids profiles of smoked catfish prepared from pond, cage culture and wild fish. Catfish samples 300-350 grams in weight wastaken respectualy from wild (Kampar river), pond and cage culture in Sungai Paku, Kampar. The fish was smoked using hot smoking method, and the smoked fish was evaluated for smoking yield, sensory quality, proximate composition, amino acid and fatty acid profile.The smoking...

  17. Information Flow along Catfish Marketing Channels in Nigeria: Whither the Role of Mobile Telephony?

    OpenAIRE

    Animashaun, Jubril Olayinka; Fakayode, S.B.; Ayinde, Opeyemi Eyitayo

    2013-01-01

    The objectives of this study were two-folds. First, examined the use of mobile phone technology to facilitate catfish marketing and second, identified the determinants of variations in the frequency of mobile phone use for catfish marketing among agents involved in the marketing chain. The study was carried out in Kwara-state, Nigeria. One hundred and forty respondents were stratified and randomly sampled and data was collected through the use of well-designed questionnaires. Descriptive and ...

  18. Genetic structure in the Amazonian catfish Brachyplatystoma rousseauxii : influence of life history strategies

    OpenAIRE

    Carvajal-Vallejos, F. M.; Duponchelle, Fabrice; Desmarais, E.; Cerqueira, F.; Quérouil, Sophie; Nunez Rodriguez, Jesus; Garcia, C.; Renno, Jean-François

    2014-01-01

    The Dorado or Plateado (Gilded catfish) Brachyplatystoma rousseauxii (Pimelodidae, Siluriformes) is a commercially valuable migratory catfish performing the largest migration in freshwaters: from the Amazonian headwaters in the Andean foothills (breeding area) to the Amazon estuary (nursery area). In spite of its importance to inform management and conservation efforts, the genetic variability of this species has only recently begun to be studied. The aim of the present work was to determine ...

  19. Success of cuckoo catfish brood parasitism reflects coevolutionary history and individual experience of their cichlid hosts

    Science.gov (United States)

    Polačik, Matej; Smith, Carl; Honza, Marcel; Reichard, Martin

    2018-01-01

    Obligate brood parasites manipulate other species into raising their offspring. Avian and insect brood parasitic systems demonstrate how interacting species engage in reciprocal coevolutionary arms races through behavioral and morphological adaptations and counteradaptations. Mouthbrooding cichlid fishes are renowned for their remarkable evolutionary radiations and complex behaviors. In Lake Tanganyika, mouthbrooding cichlids are exploited by the only obligate nonavian vertebrate brood parasite, the cuckoo catfish Synodontis multipunctatus. We show that coevolutionary history and individual learning both have a major impact on the success of cuckoo catfish parasitism between coevolved sympatric and evolutionarily naïve allopatric cichlid species. The rate of cuckoo catfish parasitism in coevolved Tanganyikan hosts was 3 to 11 times lower than in evolutionarily naïve cichlids. Moreover, using experimental infections, we demonstrate that parasite egg rejection in sympatric hosts was much higher, leading to seven times greater parasite survival in evolutionarily naïve than sympatric hosts. However, a high rejection frequency of parasitic catfish eggs by coevolved sympatric hosts came at a cost of increased rejection of their own eggs. A significant cost of catfish parasitism was universal, except for coevolved sympatric cichlid species with previous experience of catfish parasitism, demonstrating that learning and individual experience both contribute to a successful host response.

  20. Characterisation of Catfish (Clarias Batrachus) Oil: β-Cyclodextrin Inclusion Complex

    International Nuclear Information System (INIS)

    Zaibunnisa Abdul Haiyee; Nor Izzatul Adyani Yahya; Norizzah Abd Rashid; Dzulkifly Mat Hashim

    2016-01-01

    Catfish is a cheap source of essential omega-3 fatty acids especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Catfish oil was extracted and clean-up using pressurised liquid extraction (PLE) from the viscera of catfish (Clarias batrachus). However, the characteristics of catfish oils are sticky, strong fishy odour and can easily be oxidised with short shelf-life. In this study, catfish oil was converted into powder by formation of inclusion complex with β-cyclodextrin. Inclusion complex was prepared by using co-precipitation and kneading methods and compared with physical mixture. The inclusion complex formed were characterised by using field emission scanning electron microscopy (FESEM), differential scanning calorimeter (DSC) and fourier transform infrared spectroscopy (FTIR). FESEM images revealed that inclusion complex using co-precipitation and kneading methods has smaller in crystal sizes and appeared as different morphology compared to physical mixture. DSC proved that co-precipitation method was able to form new solid phase due to shifting of melting point to higher temperature (145.22 degree Celcius). FTIR supported the result by proving strengthening of carbonyl group (C=O). Therefore, co-precipitation method was able to successfully produce inclusion complex of catfish oil, β-cyclodextrin. (author)

  1. Diversity, threats and conservation of catfish fauna of the Krishna River, Sangli District, Maharashtra, India

    Directory of Open Access Journals (Sweden)

    S.M. Kumbar

    2014-01-01

    Full Text Available The diversity of freshwater catfish species of the Krishna River, Sangli District was studied from June 2009 to July 2012. The study area covered 105km from Karad City where the Koyna tributary joins the Krishna River up to Mhaishal, the state border of Maharashtra. It was divided into three streams for convenience, i.e., the upper stream starts from Karad and goes up to Takari, the middle stream from Takari to Bhilawadi and downstream from Bhilawadi to Mhaishal. A total of 13 species of catfish belonging to five families and 10 genera were recorded. The Bagridae family was dominant with six species, whereas Siluridae, Schilbidae and Clariidae had two species each and Sisoridae with one species. We have provided range extension for an endemic and threatened sisorid catfish Glyptothorax poonaensis. The occurrence and distribution of catfishes was more or less equal in number along the study area. The maximum number of species recorded was nine from the upper stream, whereas the middle and down streams had eight and seven species respectively. The distribution of catfish along the Krishna River system may be due to the slow and steady state water movement and its width that ensure the continuous availability of nutrition. It is suggested that the Krishna River would be a suitable habitat for the conservation of freshwater catfish if the threats are minimized.

  2. Effect of stocking biomass on solids, phytoplankton communities, common off-flavors, and production parameters in a channel catfish biofloc technology production system

    Science.gov (United States)

    The effect of initial channel catfish (Ictalurus punctatus, Rafinesque, 1818) fingerling biomass (1.4, 1.8, or 2.3 kg m-3) on phytoplankton communities, common off-flavors, and stocker catfish production parameters was evaluated in biofloc technology production tanks. Stocker catfish size (145.5 – 1...

  3. Discrepancy between molecular structure and ligand selectivity of a testicular follicle-stimulating hormone receptor of the African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Bogerd, J.; Blomenröhr, M.; Andersson, E.; van der Putten, H.; Tensen, C.P.; Vischer, H F; Granneman, Joke C M; Janssen-Dommerholt, C; Goos, H.J.; Schulz, Rüdiger W

    A putative FSH receptor (FSH-R) cDNA was cloned from African catfish testis. Alignment of the deduced amino acid sequence with other (putative) glycoprotein hormone receptors and analysis of the African catfish gene indicated that the cloned receptor belonged to the FSH receptor subfamily. Catfish

  4. Efeito da estratégia de vida sobre as variações no conteúdo de energia de duas espécies de peixes (Brycon hilarii e Hypophthalmus edentatus, durante o ciclo reprodutivo = Effect of life strategy on the variations of energy content of two fish species (Brycon hilarii e Hypophthalmus edentatus during their reproductive cycle

    Directory of Open Access Journals (Sweden)

    Vanessa Monteiro

    2007-04-01

    Full Text Available Com o objetivo de investigar diferenças no conteúdo de energia durante o ciclo reprodutivo, foram selecionados 54 exemplares de Brycon hilarii (k-estrategista e 82 de Hypophthalmus edentatus (r-estrategista em amostragens realizadas mensalmente, entreoutubro de 2000 e março de 2004, no reservatório de Manso, Estado do Mato Grosso do Norte e em sua área de influência. Determinou-se, para cada indivíduo, o conteúdo calórico, em kcal g-1 de peso seco, com auxílio da bomba calorimétrica (Parr 1261 e, realizaram-se comparações entre os valores calóricos obtidos, o fator de condição (K e a relação gonadossomática. Observou-se que, em geral, os valores médios de K e do conteúdo calórico foram superiores nos machos das duas espécies analisadas, embora não tenham sidoidentificadas diferenças significativas entre os sexos. Constatou-se que os valores calóricos de H. edentatus foram superiores aos de B. hilarii, enquanto os valores do fator de condição foram contrários, apresentando-se superiores para B. hilarii. Diferenças significativas foram constatadas nas comparações realizadas entre o conteúdo energético e o fator de condição, de indivíduos de ambas as espécies. Enquanto B. hilarii apresentou as mais elevadas concentrações de energia nos músculos, durante o máximo desenvolvimento gonadal, emH. edentatus, isso ocorreu quando iniciava o processo de maturação gonadal. Os resultados descritos para cada espécie durante o ciclo reprodutivo permitem confirmar a hipótese de que as duas espécies de peixes, B. hilarii e H. edentatus, com diferentes estratégias de vida,acumulam e mobilizam energia de forma distinta durante o período de maturação gonadal.The aim of this work was to investigate differences in the energy content during reproductive cycle of two species of fish with different life strategies: Brycon hilarii (k-strategist and Hypophthalmus edentatus (r-strategist. Fifty-four samples of B. hilarii

  5. Effect of alcohol and formaldehyde on the ä13C and ä15N isotopic composition of Plagioscion squamosissimus and Hypophthalmus edentatus (Pisces, Osteichthyes = Efeito do alcool e formol sobre a composicao isotopica de ƒÂ13C e de ƒÂ15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes

    Directory of Open Access Journals (Sweden)

    Gislaine Iachstel Manetta

    2011-09-01

    Full Text Available The present study investigates the ƒÂ13C and ƒÂ15N isotopic composition in frozen samples (control, samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze . control group (-15oC, conservation in alcohol 70%and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for ƒÂ13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish.O presente estudo investiga a composicao isotopica de ƒÂ13C e ƒÂ15N entre as amostras congeladas (controle, em alcool e em formol de Plagioscion squamosissimus e Hypophthalmus edentatus. De cada individuo foi extraida uma faixa de musculo localizada na regiao acima da linha lateral, na base da nadadeira dorsal, a qual foi subdividida em tres partes iguais, sendo cada uma delas submetida a um tipo de tratamento: congelamento - grupo control (-15oC, conservacao em alcool 70% e fixacao em formol 4%. As amostras foram mantidas nos

  6. Biofilm formation by Salmonella spp. in catfish mucus extract under industrial conditions.

    Science.gov (United States)

    Dhowlaghar, Nitin; De Abrew Abeysundara, Piumi; Nannapaneni, Ramakrishna; Schilling, Mark W; Chang, Sam; Cheng, Wen-Hsing; Sharma, Chander S

    2018-04-01

    The objective of this study was to determine the effect of strain and temperature on the growth and biofilm formation of Salmonella spp. in high and low concentrations of catfish mucus extract on different food-contact surfaces at 22 °C and 10 °C. The second objective of this study was to evaluate the efficacy of disinfectants at recommended concentrations and contact times for removing Salmonella biofilms cells on a stainless steel surface containing catfish mucus extract. Growth and biofilm formation of all Salmonella strains increased with higher concentrations of catfish mucus extract at both 10 °C and 22 °C. In 15 μg/ml of catfish mucus extract inoculated with 3 log CFU/ml, the biofilm levels of Salmonella on stainless steel surface reached to 3.5 log CFU/cm 2 at 10 °C or 5.5 log CFU/cm 2 at 22 °C in 7 days. In 375 μg/ml of catfish mucus extract inoculated with 3 log CFU/ml, the biofilm levels of Salmonella on the stainless steel surface reached 4.5 log CFU/cm 2 at 10 °C and 6.5 log CFU/cm 2 at 22 °C in 7 days. No differences were observed between Salmonella strains tested for biofilm formation in catfish mucus extract on the stainless steel surface. The biofilm formation by Salmonella Blockley (7175) in catfish mucus extract was less (P stainless steel, polyethylene and polyurethane surfaces. Salmonella biofilm cells were not detectable on the stainless steel surface after treatment with a mixture of disinfectants but were still present when single compound disinfectants were used. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Emergence of Edwardsiella piscicida in farmed channel femal, Ictalurus punctatus X blue male, Ictalurus furcatus, hybrid catfish cultured in Mississippi

    Science.gov (United States)

    here is a trend toward the increased incidence and prevalence of Edwardsiella piscicida septicemia in US catfish aquaculture, particularly in channel ', Ictalurus punctatus, × blue ', I. furcatus, hybrid catfish. From 2013 to 2017, a total of 3242 disease case submissions were made to the Aquatic Re...

  8. Evaluation of potassium permanganate against an experimental subacute infection of Flavobacterium columnare in channel catfish, Icatlurus punctatus

    Science.gov (United States)

    The efficacy of potassium permanganate (KMnO4) as a prophylactic and therapeutic treatment for subacute infection of Flavobacterium columnare was demonstrated in experimentally infected channel catfish, Ictalurus punctatus. Catfish experimentally infected with F. columnare to mimic a subacute infec...

  9. Dioxin congener patterns in commercial catfish from the United States and the indication of mineral clays as the potential source

    Science.gov (United States)

    Since 1991 the U.S. Department of Agriculture (USDA) has conducted annual surveys of pesticide residues in foods under the Agricultural Marketing Service’s Pesticide Data Program (PDP). To assess chemical residues in domestically marketed catfish products, 1479 catfish samples were collected during ...

  10. The potential for using red claw crayfish and hybrid African catfish as biological control agents for Schistosoma host snails

    NARCIS (Netherlands)

    Monde, C.; Syampungani, S.; Rico, A.; Brink, van den P.J.

    2017-01-01

    The potential of red claw crayfish and hybrid African catfish (Clarias gariepinus and Clarias ngamensis) as predators for Schistosoma host snails was evaluated in 2014 by monitoring the consumption of snails by crayfish and catfish in experimental tanks over time under laboratory conditions. After

  11. Blood flukes (Digenea: Aporocotylidae) of walking catfishes (Siluriformes: Clariidae): new genus and species from the Mekong River (Vietnam) with comments on related catfish aporocotylids.

    Science.gov (United States)

    Truong, Triet Nhat; Bullard, Stephen A

    2013-07-01

    Nomasanguinicola canthoensis gen. et sp. n. infects the branchial vessels of bighead catfish, Clarias macrocephalus Günther (Siluriformes: Clariidae), in the Mekong River near Can Tho, southern Vietnam. Nomasanguinicola differs from all other genera of fish blood flukes (Digenea: Aporocotylidae) by the combination of lacking body spines and by having an anterior sucker with two flanking columns of large denticles, an intestine comprising several short papilla-like caeca, an inverse U-shaped uterus, and an ootype located near the separate genital pores. The new species has an ootype that is posterior to the level of the female genital pore. That feature most easily differentiates it from the only other putative aporocotylid species having an anterior sucker with two flanking columns of large denticles, Plehniella dentata Paperna, 1964 and Sanguinicola clarias Imam, Marzouk, Hassan et Itman, 1984, which have an ootype that is lateral (P. dentata) or anterior (S. clarias) to the level of the female genital pore. These two species apparently lack extant type materials, infect North African catfish, Clarias gariepinus (Burchell), and herein are considered incertae sedis, but likely comprise species of Nomasanguinicola. An updated list of hosts, sites of infection and geographic localities for the six species and three genera of blood flukes that mature in catfishes is provided. The new species is the first fish blood fluke recorded from Vietnam and only the third reported from a walking catfish (Clariidae).

  12. Capability of Catfish (Clarias gariepinus to Accumulate Hg2+ From Water

    Directory of Open Access Journals (Sweden)

    Heny Suseno

    2015-12-01

    Full Text Available Mercury is hazardous contaminant that can be accumulated by aquatic organisms such as fishes, mussels etc. Catfish is one of source of animal protein but it also can accumulate Hg2+ from water that used in aquaculture. Due to less information about capability of catfish to accumulate Hg2+, therefore we studied bioaccumulation of Hg2+ that used biokinetic approach (aqueous uptake-rate, and elimination-rate.  Nuclear application technique was applied in this study by using radiotracer of 203Hg.  A simple kinetic model was then constructed to predict the bioaccumulation capability of   by catfish. The result of experiments were shown that the uptake rate of difference Hg2+ concentration were 79.90 to 101.22 ml.g-1.d-1. Strong correlation between uptake rates with increasing Hg2+concentration. In addition, the elimination rates were range 0.080 – 0.081 day-1. The biology half time (t1/2b of Hg2+ in whole body catfish were 8.50 – 8.63 days.  However, no clear correlation  between elimination rate with increasing concentration of Hg2+. The calculation of Bio Concentration Factor (BCF shown catfish have capability to accumulated Hg maximum 1242.69 time than its concentration in water

  13. Protein Hydrolysis from Catfish Prepared by Papain Enzyme and Antioxidant Activity of Hydrolyzate

    Directory of Open Access Journals (Sweden)

    Ace Baehaki

    2015-12-01

    Full Text Available The objective of this research was to make a protein hydrolysates from catfish (Pangasius pangasius enzymatically using papain enzyme and analyzed the antioxidant activity of protein hydrolysates produced. The research used the method completely randomized design with two replications the treatment were the difference concentration of the papain enzyme (0%, 1%, 2%, 3%, 4%, 5%, and 6%. The parameters of research were antioxidative activity using DPPH (2,2-difenil-1–pikrilhidrazil, protein content, and molecular weight using SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. The results showed that catfish protein hydrolysates prepared by papain enzyme has antioxidative activity. The highest degree of hydrolysis was 71.98% at enzyme concentration of 6%. Based on the DPPH scavenging method catfish protein hydrolysates has the antioxidative activity with the value 37.85-67.62%. The protein content of catfish protein hydrolysates were 20.86-54.47 mg/ml. The molecular weight of catfish protein hydrolyzates were 11.90-65.20 kDa.

  14. The efficacy of mass-marking channel catfish fingerlings by immersion in oxytetracycline

    Science.gov (United States)

    Stewart, David R.

    2011-01-01

    Oxytetracycline (OTC) has been extensively used for marking a variety of fish species, but has never been successfully used to mark channel catfish Ictalurus punctatus. Channel catfish fingerlings (~ 25 mm TL) obtained from the Oklahoma Department of Wildlife Conservation at Byron Fish Hatchery were kept in Living Streams (791 to 1,018 L) equipped with recirculation units. Marking trials consisted of immersing channel catfish in one of three concentrations (250, 450, and 700 mg/L) OTC hydrochloride [HCl] for 6 hours. Samples of channel catfish were obtained from each group at 1-week and 4-week postimmersion. Lapilli otoliths and pectoral spines were removed to assess mark presence with an epi-fluorescent compound microscope. After one week, no marks were detected on pectoral spines for all treatments, mark detection on otoliths depended on concentration, but never exceeded 43% (700 mg/L). After four weeks, all otoliths and pectoral spines were determined marked for 700 mg/L OTC, 20% for fish immersed in 450 mg/L OTC, and 0% were marked after four weeks at the 250 mg/L OTC. Results show, channel catfish fingerlings can be successfully marked with immersion in OTC at 700 mg/L for at least 6 hours.

  15. Age, growth and fall diet of channel catfish in Cheat Lake, West Virginia

    Science.gov (United States)

    Hilling, Corbin D.; Welsh, Stuart A.; Smith, Dustin M.

    2016-01-01

    Acidification has historically impaired Cheat Lake's fish community, but recent mitigation efforts within the Cheat River watershed have improved water quality and species richness. Presently, channel catfish Ictalurus punctatus are abundant and attain desirable sizes for anglers. We evaluated the age, growth, and fall diet of the population. We collected a sample of 155 channel catfish from Cheat Lake from 5 August to 4 December 2014, a subset of which we aged (n = 148) using lapillus otoliths. We fit four growth models (von Bertalanffy, logistic, Gompertz, and power) to length-at-age data and compared models using an information theoretic approach. We collected fall diets from 55 fish sampled from 13 October to 4 December 2014. Total lengths of individuals in the sample ranged from 154 to 721 mm and ages ranged from 2 to 19 y. We AICc-selected the von Bertalanffy growth model as the best approximating model, and the power and Gompertz models also had considerable support. Diets were numerically dominated by Diptera larvae, specifically Chironomidae and Chaoboridae, while 39% of stomachs contained terrestrial food items. This study provides baseline data for management of Cheat Lake's channel catfish population. Further, this study fills a knowledge gap in the scientific literature on channel catfish, because few previously published studies have examined the population ecology of channel catfish in the Central Appalachian region.

  16. Seasonal food habits of introduced blue catfish in Lake Oconee, Georgia

    Science.gov (United States)

    Jennings, Cecil A.; Mitchell, Geoffrey E.; Nelson, Chris

    2018-01-01

    Blue catfish (Ictalurus furcatus) are native to the Coosa River drainage in northwest Georgia but have been widely introduced outside of this range including Lake Oconee, a 7677-ha impoundment on the Oconee River in central Georgia. Blue catfish abundance and growth rates have increased dramatically since their introduction in Lake Oconee, but their food habits are unknown. Therefore, food habits of blue catfish in this impoundment were determined by examining the stomachs of 808 specimens in the reservoir’s upper and lower regions across all seasons from summer 2012 to summer 2013. Diet was summarized using the Relative Importance of specific prey by weight. In the upper region of the reservoir, Asian clams (Corbicula fluminea) were the dominant prey item during the summer (75.7%), fall (66.4%), and winter (37.6%); whereas crappie (Pomoxis spp.) was the dominant prey item in the spring (38.7%). Asian clams also were the dominant prey items in the lower region during the fall (68.4%), winter (33.9%), and spring (36.4%). Blue catfish seemed to feed opportunistically on seasonally abundant prey items in both the upper riverine and lower lacustrine portions of the reservoir. Of the many sportfishes in the reservoir, only crappie was an important prey item, and then only in the upper region during the spring. Our results do not support concerns that blue catfish are an apex predator that would decimate the sportfish assemblage in this recently colonized reservoir.

  17. Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies

    Energy Technology Data Exchange (ETDEWEB)

    Catfish Genome Consortium; Wang, Shaolin; Peatman, Eric; Abernathy, Jason; Waldbieser, Geoff; Lindquist, Erika; Richardson, Paul; Lucas, Susan; Wang, Mei; Li, Ping; Thimmapuram, Jyothi; Liu, Lei; Vullaganti, Deepika; Kucuktas, Huseyin; Murdock, Christopher; Small, Brian C; Wilson, Melanie; Liu, Hong; Jiang, Yanliang; Lee, Yoona; Chen, Fei; Lu, Jianguo; Wang, Wenqi; Xu, Peng; Somridhivej, Benjaporn; Baoprasertkul, Puttharat; Quilang, Jonas; Sha, Zhenxia; Bao, Baolong; Wang, Yaping; Wang, Qun; Takano, Tomokazu; Nandi, Samiran; Liu, Shikai; Wong, Lilian; Kaltenboeck, Ludmilla; Quiniou, Sylvie; Bengten, Eva; Miller, Norman; Trant, John; Rokhsar, Daniel; Liu, Zhanjiang

    2010-03-23

    Background-Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpose of SNP identification. Results-A total of 438,321 quality ESTs were generated from 8 channel catfish (Ictalurus punctatus) and 4 blue catfish (Ictalurus furcatus) libraries, bringing the number of catfish ESTs to nearly 500,000. Assembly of all catfish ESTs resulted in 45,306 contigs and 66,272 singletons. Over 35percent of the unique sequences had significant similarities to known genes, allowing the identification of 14,776 unique genes in catfish. Over 300,000 putative SNPs have been identified, of which approximately 48,000 are high-quality SNPs identified from contigs with at least four sequences and the minor allele presence of at least two sequences in the contig. The EST resource should be valuable for identification of microsatellites, genome annotation, large-scale expression analysis, and comparative genome analysis. Conclusions-This project generated a large EST resource for catfish that captured the majority of the catfish transcriptome. The parallel analysis of ESTs from two closely related Ictalurid catfishes should also provide powerful means for the evaluation of ancient and recent gene duplications, and for the development of high-density microarrays in catfish. The inter- and intra-specific SNPs identified from all catfish EST dataset assembly will greatly benefit the catfish introgression breeding program and whole genome association studies.

  18. Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.

    Directory of Open Access Journals (Sweden)

    Ali Akgul

    Full Text Available Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7 were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.

  19. The physico-chemical properties of pangas catfish (Pangasius pangasius) skin gelatin

    Science.gov (United States)

    Pradarameswari, K. A.; Zaelani, K.; Waluyo, E.; Nurdiani, R.

    2018-04-01

    Gelatin can be used as emulsifier and stabilizer in food products. Until now, the most widely used raw materials for the production of gelatin industry are cow bone, cow skin and pig skin. Fish gelatin has been highlighted as a better alternative to replace mammals gelatin based on ethical and religious perspective. Fish gelatin was extracted from Pangas catfish skin to determine its physico-chemical properties. Different temperatures (45 °C, 50 °C, 55 °C) were employed during gelatin extraction. Higher temperature increased the yield and fat contents of Pangas catfish skin gelatin. In contrary, higher water, protein, ash contents were observed during lower temperature. Temperature significantly (p fish skin gelatin. Based on the FTIR spectrum catfish skin gelatin functional groups can be identified as N-H, O-H, C = H, C-O and C-H.

  20. Spatial variability and macro‐scale drivers of growth for native and introduced Flathead Catfish populations

    Science.gov (United States)

    Massie, Danielle L.; Smith, Geoffrey; Bonvechio, Timothy F.; Bunch, Aaron J.; Lucchesi, David O.; Wagner, Tyler

    2018-01-01

    Quantifying spatial variability in fish growth and identifying large‐scale drivers of growth are fundamental to many conservation and management decisions. Although fish growth studies often focus on a single population, it is becoming increasingly clear that large‐scale studies are likely needed for addressing transboundary management needs. This is particularly true for species with high recreational value and for those with negative ecological consequences when introduced outside of their native range, such as the Flathead Catfish Pylodictis olivaris. This study quantified growth variability of the Flathead Catfish across a large portion of its contemporary range to determine whether growth differences existed between habitat types (i.e., reservoirs and rivers) and between native and introduced populations. Additionally, we investigated whether growth parameters varied as a function of latitude and time since introduction (for introduced populations). Length‐at‐age data from 26 populations across 11 states in the USA were modeled using a Bayesian hierarchical von Bertalanffy growth model. Population‐specific growth trajectories revealed large variation in Flathead Catfish growth and relatively high uncertainty in growth parameters for some populations. Relatively high uncertainty was also evident when comparing populations and when quantifying large‐scale patterns. Growth parameters (Brody growth coefficient [K] and theoretical maximum average length [L∞]) were not different (based on overlapping 90% credible intervals) between habitat types or between native and introduced populations. For populations within the introduced range of Flathead Catfish, latitude was negatively correlated with K. For native populations, we estimated an 85% probability that L∞ estimates were negatively correlated with latitude. Contrary to predictions, time since introduction was not correlated with growth parameters in introduced populations of Flathead Catfish

  1. Sound production and pectoral spine locking in a Neotropical catfish (Iheringichthys labrosus, Pimelodidae

    Directory of Open Access Journals (Sweden)

    Javier S. Tellechea

    Full Text Available Catfishes may have two sonic organs: pectoral spines for stridulation and swimbladder drumming muscles. The aim of this study was to characterize the sound production of the catfish Iheringichthys labrosus. The I. labrosus male and female emits two different types of sounds: stridulatory sounds (655.8 + 230 Hz consisting of a train of pulses, and drumming sounds (220 + 46 Hz, which are composed of single-pulse harmonic signals. Stridulatory sounds are emitted during abduction of the pectoral spine. At the base of the spine there is a dorsal process that bears a series of ridges on its latero-ventral surface, and by pressing the ridges against the groove (with an unspecialized rough surface during a fin sweep, the animal produce a series of short pulses. Drumming sound is produced by an extrinsic sonic muscle, originated on a flat tendon of the transverse process of the fourth vertebra and inserted on the rostral and ventral surface of the swimbladder. The sounds emitted by both mechanisms are emitted in distress situation. Distress was induced by manipulating fish in a laboratory tank while sounds were recorded. Our results indicate that the catfish initially emits a stridulatory sound, which is followed by a drumming sound. Simultaneous production of stridulatory and drumming sounds was also observed. The catfish drumming sounds were lower in dominant frequency than stridulatory sounds, and also exhibited a small degree of dominant frequency modulation. Another behaviour observed in this catfish was the pectoral spine locking. This reaction was always observed before the distress sound production. Like other authors outline, our results suggest that in the catfish I. labrosus stridulatory and drumming sounds may function primarily as a distress call.

  2. Complete mitochondrial genome of the Freshwater Catfish Rita rita (Siluriformes, Bagridae).

    Science.gov (United States)

    Lashari, Punhal; Laghari, Muhammad Younis; Xu, Peng; Zhao, Zixia; Jiang, Li; Narejo, Naeem Tariq; Deng, Yulin; Sun, Xiaowen; Zhang, Yan

    2015-01-01

    The complete mitochondrial genome of Catfish, Rita rita, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red Listed species. The complete mitogenome was 16,449 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 33.40% A, 27.43% C, 14.26% G and 24.89% T. The complete mitochondrial genome of catfish, Rita rita provides the basis for genetic breeding and conservation studies.

  3. Streptococcus agalactiae impairs cerebral bioenergetics in experimentally infected silver catfish.

    Science.gov (United States)

    Baldissera, Matheus D; Souza, Carine F; Parmeggiani, Belisa S; Santos, Roberto C V; Leipnitz, Guilhian; Moreira, Karen L S; da Rocha, Maria Izabel U M; da Veiga, Marcelo L; Baldisserotto, Bernardo

    2017-10-01

    It is becoming evident that bacterial infectious diseases affect brain energy metabolism, where alterations of enzymatic complexes of the mitochondrial respiratory chain and creatine kinase (CK) lead to an impairment of cerebral bioenergetics which contribute to disease pathogenesis in the central nervous system (CNS). Based on this evidence, the aim of this study was to evaluate whether alterations in the activity of complex IV of the respiratory chain and CK contribute to impairment of cerebral bioenergetics during Streptococcus agalactiae infection in silver catfish (Rhamdia quelen). The activity of complex IV of the respiratory chain in brain increased, while the CK activity decreased in infected animals compared to uninfected animals. Brain histopathology revealed inflammatory demyelination, gliosis of the brain and intercellular edema in infected animals. Based on this evidence, S. agalactiae infection causes an impairment in cerebral bioenergetics through the augmentation of complex IV activity, which may be considered an adaptive response to maintain proper functioning of the electron respiratory chain, as well as to ensure ongoing electron flow through the electron transport chain. Moreover, inhibition of cerebral CK activity contributes to lower availability of ATP, contributing to impairment of cerebral energy homeostasis. In summary, these alterations contribute to disease pathogenesis linked to the CNS. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Reproductive strategy of the Patagonian catfish Hatcheria macraei.

    Science.gov (United States)

    Chiarello-Sosa, J M; Battini, M A; Barriga, J P

    2016-09-01

    This study describes the reproductive strategy of the stream-dwelling catfish Hatcheria macraei in the Pichileufu River, Argentina. Gonad maturity phases, classified on the basis of histological analysis, stages of gamete development and the frequency distribution of oocyte size, were correlated with macroscopic features of the gonads. Hatcheria macraei has a cystovarian ovary, asynchronous oocyte development and lobular testes. Five oocyte and four spermatogenic stages were identified and related to macroscopic gonad characteristics, making it possible to divide gonad development into five phases for females and males. Mature oocyte diameter ranged from 922 to 1935 µm. Absolute fecundity in mature females varied from 115 to 480 oocytes. Hatcheria macraei has multiple spawning during a protracted reproductive season that extends from December to April. This, together with its small size, is characteristic of an opportunistic reproductive strategy, commonly found in species that inhabit adverse and unpredictable environments, such as the low-order rivers of Patagonia. © 2016 The Fisheries Society of the British Isles.

  5. Population structure of the Southeast Asian river catfish Mystus nemurus.

    Science.gov (United States)

    Usmani, S; Tan, S G; Siraj, S S; Yusoff, K

    2003-12-01

    A total of 143 microsatellites were isolated from Mystus nemurus using a 5' anchored polymerase chain reaction technique or the random amplified hybridization microsatellite method, the first set of microsatellite markers developed for the Southeast Asian river catfish. Twenty polymorphic microsatellite loci were used as markers for population characterization of M. nemurus from six different geographical locations in Malaysia (Perak, Kedah, Johor, UPM, Sarawak and Terengganu). The number of alleles per locus ranged from 2 to 11 with 6.3 as the average number of alleles per locus. Characterization of the populations showed relatively high levels of genetic variation compared with previous studies using allozyme markers. The highest genetic similarity was found between Perak and Kedah, while the highest genetic distance was found between Terengganu and Kedah. The majority of clustering was in accordance with geographical locations and the histories of the populations. Microsatellite analysis indicated that the Sarawak population might be genetically closer to the Peninsular Malaysian populations than has been previously shown by other molecular marker studies.

  6. Proteomic profiling of white muscle from freshwater catfish Rita rita.

    Science.gov (United States)

    Mohanty, Bimal Prasanna; Mitra, Tandrima; Banerjee, Sudeshna; Bhattacharjee, Soma; Mahanty, Arabinda; Ganguly, Satabdi; Purohit, Gopal Krishna; Karunakaran, Dhanasekar; Mohanty, Sasmita

    2015-06-01

    Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for many aspects of aquaculture including fish physiology and growth, flesh quality, food safety and aquatic environmental monitoring. The freshwater catfish Rita rita of the family Bagridae inhabiting the tropical rivers and estuaries is an important food fish with high nutritive value and is also considered a species of choice in riverine pollution monitoring. Omics information that could enhance utility of this species in molecular research is meager. Therefore, in the present study, proteomic analysis of Rita rita muscle has been carried out and functional genomics data have been generated. A reference muscle proteome has been developed, and 23 protein spots, representing 18 proteins, have been identified by MALDI-TOF/TOF-MS and LC-MS/MS. Besides, transcript information on a battery of heat shock proteins (Hsps) has been generated. The functional genomics information generated could act as the baseline data for further molecular research on this species.

  7. Antibacterial Resistance in African Catfish Aquaculture: a Review

    Directory of Open Access Journals (Sweden)

    Madubuike U. ANYANWU

    2016-03-01

    Full Text Available Antibacterial resistance (AR is currently one of the greatest threats to mankind as it constitutes health crisis. Extensive use of antibacterial agents in human and veterinary medicine, and farm crops have resulted in emergence of antibacterial-resistant organisms in different environmental settings including aquaculture. Antibacterial resistance in aquaculture is a serious global concern because antibacterial resistance genes (ARGs can be transferred easily from aquaculture setting to other ecosystems and the food chain. African catfish (ACF aquaculture has increased at a phenomenal rate through a continuous process of intensification, expansion and diversification. Risk of bacterial diseases has also increased and consequently there is increased use of antibacterial agents for treatment. Antibacterial resistance in ACF aquaculture has huge impact on the food chain and thus represents risk to public and animal health. In “one health” approach of curbing AR, knowledge of the sources, mechanisms and magnitude of AR in ACF aquaculture and its potential impact on the food chain is important in designing and prioritizing monitoring programs that may generate data that would be relevant for performing quantitative risk assessments, implementation of antibacterial stewardship plans, and developing effective treatment strategies for the control of ACF disease and reducing risk to public health. This review provides insight on the sources, mechanisms, prevalence and impact of antibacterial resistance in ACF aquaculture environment, a setting where the impact of AR is neglected or underestimated.

  8. Earthy and musty off-flavor episodes in catfish split-pond aquaculture systems

    Science.gov (United States)

    The interest and use of variations of partitioned aquaculture systems (PAS) by the southeastern U.S. catfish farming industry continues to grow. Split-pond systems, one type of PAS, are designed to improve management of dissolved oxygen levels and fish waste products (e.g., ammonia) compared to conv...

  9. Maternal Transfer of Vitamin C in Channel Catfish (Ictalurus Punctatus) Effects Reproduction and Progeny Performance

    Science.gov (United States)

    Two routes of maternal transfer of vitamin C in channel catfish female broodfish prior to spawning were explored as a strategy to incorporate the vitamin to determine its effect on reproduction and subsequent performance of the progeny. Accumulation of vitamin C was higher (p<0.05) in ovarian tissu...

  10. Biofilm formation by Salmonella spp. in catfish mucus extract under industrial conditions

    Science.gov (United States)

    The objective of this study was to determine the effect of strain and temperature on the growth and biofilm formation of Salmonella spp. in high and low concentrations of catfish mucus extract on different food-contact surfaces at 22°C and 10°C. The second objective of this study was to evaluate the...

  11. Food and condition of the catfish Synodontis in Upper Benue River ...

    African Journals Online (AJOL)

    Investigation was carried out on food and condition of the Catfish, Synodontis species in the Upper Benue River Basin, Nigeria from March to June, 2000. Fish samples were obtained twice monthly from different fish landing sites. Analysis showed that there was no significant difference between the sizes of males and ...

  12. Biomarker assessment of the effects of strip mine contamination on channel catfish

    International Nuclear Information System (INIS)

    Martin, L.K. Jr.; Black, M.C.

    1994-01-01

    A suite of biomarkers were used to evaluate acute (1 day) to semi-chronic (3 month) heavy metal-induced toxicity in channel catfish, Ictalurus punctatus, caged at an abandoned strip mine and a noncontaminated reference site. Assays performed include indicators of metabolic, hematological, osmoregulatory, and genotoxic stress. Two cage designs were utilized to evaluate the importance of exposure routes; one allowing exclusive contact with the water column and the other allowing contact with water and sediments. Significant DNA strand breakage was observed in catfish exposed in both regimes, but DNA repair was observed only in water-exposed catfish. Transient increases in hemoglobin, ALAD, and hematocrit levels were observed at 1 month for both exposure regimes, followed by a return to control levels for the duration of the study. Environmental conditions (i.e., changes in water quality and temperature) may have contributed to the variable plasma chloride and glucose levels observed in all catfish exposed to strip mine wastes. Further analysis of the data with non-metric clustering techniques and correlations with tissue metal residues may yield more insights into causal relationships

  13. In situ assessment of two catfish species (pisces, Ariidae) to evaluate pollution in a harbor

    Science.gov (United States)

    Neta, Raimunda Nonata Fortes Carvalho; Junior, Audalio Rebelo Torres; Sousa, Débora Batista Pinheiro; de Sousa de Oliveira Mota Andrade, Ticianne; Torres, Hetty Salvino; da Silva Castro, Jonatas; da Silva de Almeida, Zafira; Santos, Débora Martins Silva; Tchaicka, Lígia

    2016-12-01

    A histopathological and biometric database for the catfish Sciades herzbergii and Bagre bagre from São Luís Island (Harbor area) and Caranguejos Island (reference area) in Brazil is presented. Branchial and hepatic lesions were classified into three reaction patterns: 1) circulatory or inflammatory disturbances; 2) regressive changes; 3) progressive changes. The total length (Lt), standard length (Ls), furcal length (Lf), total weight (Wt), and gonad weight (Wg) of each fish were recorded. As expected, most populations of catfish considered in this study are highly heterogeneous, with lengths and weights deviating from the reference sample. No histopathological lesions were observed in Sciades herzbergii examined at the reference site (Caranguejos Island). In contrast, 90% of the catfish S. herzbergii from sites located in the Harbor Area (São Luís Island) had one or more types of branchial and hepatic lesions. As opposed to what was observed in S. herzbergii, more than 86.33% of Bagre bagre individuals showed histopathological alterations in both areas. The utility of histopathological lesions and biometric data as sensitive indicators of the health of wild catfish populations has been demonstrated. Sciades herzbergii proved to be a better species for biomonitoring because it was able to differentiate one impacted site (Port Area/ São Luís Island) from a region relatively free of contaminants (Reference Area/ Caranguejos Island).

  14. Structure of the Helminth Assemblage of and Endemic Madtom Catfish (Noturus Lachneri)

    Science.gov (United States)

    Riccardo A. Fiorillo; R. Brent Thomas; Melvin L. Warren; Christopher M. Taylor

    1999-01-01

    The Ouachita madtom, Noturus lachneri, is a small, uniformly-colored catfish endemic to the upper Saline and Ouachita river drainages in central Arkansas (Robison and Buchanan, 1988), where it is often found in shallow pools associated with clear, high gradient, rock-bottomed streams (Robison and Harp, 1985). Distribution, habitat, diet, and conservation status of...

  15. Utilization of solid catfish manure waste as carbon and nutrient source for lactic acid production.

    Science.gov (United States)

    Shi, Suan; Li, Jing; Blersch, David M

    2018-04-19

    The aim of this work was to study the solid waste (manure) produced by catfish as a potential feedstock for the production of lactic acid (LA) via fermentation. The solid waste contains high levels of both carbohydrates and nutrients that are sufficient for LA bacteria. Simultaneous saccharification and co-fermentation (SSCF) was applied using enzyme and Lactobacillus pentosus, and different loadings of enzyme and solid waste were tested. Results showed LA concentrations of 35.7 g/L were obtained at 15% solids content of catfish waste. Because of the high nutrient content in the fish waste, it could also be used as supplementary substrate for nitrogen and carbon sources with other lignocellulosic materials. A combined feedstock of catfish waste and paper mill sludge was tested, increasing the final LA concentration to 43.1 g/L at 12% solids loading. The catfish waste was shown to be a potential feedstock to provide both carbon and nutrients for LA production, suggesting its use as a sole substrate or in combination with other lignocellulosic materials.

  16. The South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 (Pisces, Siluriformes, Loricariidae)

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1976-01-01

    Two species of South American Mailed Catfishes of the genus Pseudoloricaria Bleeker, 1862 are redescribed and figured from type-specimens and additional material: Pseudoloricaria laeviuscula (Valenciennes, 1840), and Pseudoloricaria punctata (Regan, 1904). Since the provenance of the holotype of P.

  17. Three new South American mailed catfishes of the genera Rineloricaria and Loricariichthys (Pisces, Siluriformes, Loricariide)

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1979-01-01

    Three new species belonging to two different genera of South American mailed catfishes of the subfamily Loricariinae are described and figured. A discussion of and comparative notes on related species are added. Rineloricaria formosa n. sp. is described from the Río Inírida/Río Orinoco drainage in

  18. Stress Effects in Channel Catfish (Ictalurus punctatus) Fry on Pond Survival

    Science.gov (United States)

    Results from previous studies suggested that channel catfish (Ictalurus punctatus) fry that were housed in very high traffic areas from hatch until reaching 9 g in size were able to survive experimental challenge with Edwardsiella ictaluri better than siblings who were hatched and raised in very low...

  19. Critical thermal maxima of two geographic strains of channel and hybrid catfish

    Science.gov (United States)

    Critical thermal maxima have been used extensively to provide physiologically and ecologically valuable reference points that identify early signs of thermal stress. In catfish pond culture, daily temperature maxima up to 36'C and daily fluctuations of as much as 6'Care observed. These extreme condi...

  20. The African catfish, Clarias gariepinus, a model for the study of reproductive endocrinology in teleosts

    NARCIS (Netherlands)

    Oordt, P.G.W.J. van; Goos, H.J.Th.

    1987-01-01

    In their natural habitat African catfish, Clarias gariepinus, show a discontinuous reproductive cycle. This cycle follows changes in the gonadotropic activity of the pituitary. Gonadotropin release has been shown to be under dual hypothalamic control, i.e. a gonadotropin-releasing hormone (GnRH) and

  1. Streptococcal Arthritis, Osteolysis, Myositis, and Spinal Meningitis in Channel Catfish Ictalurus punctatus Broodstock

    Science.gov (United States)

    This report details findings of an investigation into complaints by commercial fingerling producers of low-grade mortalities, poor reproductive success, emaciation, skin lesions, and severely arched backs among broodstock of channel catfish Ictalurus punctatus. Gross lesions involved the jaw, fin ba...

  2. Treating a natural outbreak of columnaris in channel catfish with copper sulfate and potassium permanganate

    Science.gov (United States)

    An F. Columnare-exclusive epizootic occurred in fingerling channel catfish (Ictalurus punctatus) during normal tank culture practices at SNARC. Fish were transferred to the ultra low-flow system and 2.1 mg/L copper sulfate or 3 mg/L potassium permanganate was administered; an untreated control was ...

  3. Effectiveness of copper sulfate and potassium permanganate on channel catfish infected with Flavobacterium columnare

    Science.gov (United States)

    Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were evaluated for their effectiveness to curtail mortality and decrease bacterial load in fish tissues and water in channel catfish Ictalurus punctatus naturally infected with Flavobacterium columnare, the causative agent of columnaris. Fis...

  4. Sequence genomic organization and expression of two channel catfish Ictalurus punctatus Ghrelin receptors

    Science.gov (United States)

    Two ghrelin receptor (GHS-R) genes were isolated from channel catfish tissue and a bacterial artificial chromosome (BAC) library. The two receptors were characterized by determining tissue distribution, ontogeny of receptor mRNA expression, and effects of exogenous homologous ghrelin administration ...

  5. Influence of native catfish mucus on Flavobacterium columnare growth and proteolytic activity

    Science.gov (United States)

    Flavobacterium columnare causes columnaris disease of farmed and wild freshwater fish. Skin mucus is an important factor in early stages of columnaris pathogenesis, albeit little studied. Our objectives were to 1) characterize the terminal glycosylation pattern (TGP) of catfish mucus, 2) determine t...

  6. Impact of Copper Sulfate on Plankton in Channel Catfish Nursery Ponds

    Science.gov (United States)

    Many fish culturists are interested in applying copper sulfate pentahydrate (CSP) to channel catfish, Ictalurus punctatus, nursery ponds as a prophylactic treatment for trematode infection and proliferative gill disease by killing snails and Dero sp., respectively, before stocking fry. However, copp...

  7. CHANNEL CATFISH INDUSTRY IN THE USA AND THE OFF-FLAVOR PROBLEM

    Directory of Open Access Journals (Sweden)

    Nikola Fijan

    2000-03-01

    Full Text Available The history, the production technology in channel catfish pond farming industry as well as the statistical data on production, processing and product value during past 15 years are presented. The trend of increasing consumption by the population and the presently low prices of grain and soybeans are conductive to further expansion of production. The steady growth of the industry is stimulated by several factors: innovative efforts by farmers, research at the universities and at government institutions some of which have numerous experimental ponds, cooperative extension service for farmers, modern marketing, activities of catfish farming associations, high quality of products from processing plants and vertical integration. The off-flavor in catfish caused by algal metabolites is a major problem in the industry. Genera of algae producing such metabolites, their accumulation in other fish and occurrence in drinking water reservoirs as well as the current emphasis on preventing the entrance of off-flavor contaminated catfish onto the market were reviewed. The main undesirable algal metyabolites are volatile alcohols geosmin and 2-methylisoborneol (MIB. The need for less expensive and quick methods of identifying major off-flavor compounds was pointed out. Research at the University of Arkansas at Pine Bluff, USA, on control of off-flavor algae in experimental ponds by filter-feeding silver carp (Hypophthalmichthys molitrix Val. and tilapias confined in cages showed this approach to be rather promising.

  8. Effects of oxytocin on semen release response in African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Viveiros, A.T.M.; Jatzkowski, A.; Komen, J.

    2003-01-01

    In silurid fishes, semen collection is practically impossible, even after hormonal stimulation. Instead, males are killed and testes macerated to obtain sperm. To understand the endocrine control of semen release in catfishes, we investigated the role of smooth muscle contractors in semen release

  9. MORPHOLOGICAL CHARACTERIZATION OF THE AFRICAN CATFISH (Clarias gariepinus BURCHELL, 1822 STRAINS INTRODUCED TO INDONESIA

    Directory of Open Access Journals (Sweden)

    Bambang Iswanto

    2015-12-01

    Full Text Available African catfish (Clarias gariepinus Burchell, 1822 has become a great important species in Indonesian aquaculture. Several strains of the African catfish have been introduced to Indonesia for aquaculture purposes, initiated by Dumbo strain from Taiwan in 1985, followed by Paiton strain from Thailand in 1998, then Egypt strain from Egypt in 2007, Masamo strain from Thailand in 2010 and later Kenya strain from Kenya in 2011. Since its introductions, there were no reports yet on their characterization studies. The present study was conducted to morphologically characterize the strains of African catfish introduced to Indonesia, i.e. Dumbo, Paiton, Egypt, Masamo, and Kenya strains. Morphometric and meristic data obtained were analyzed using Principal Component Analysis. Results of the morphometric characterization in the present study revealed that Dumbo, Paiton, Masamo, and Kenya strains were indistinguishable, while morphometric characteristic of Egypt strain was more or less different from those of the other strains. On the other hand, results of the meristic characterization suggested that meristic characteristics of all strains of the introduced African catfish were not different from each other. Therefore, to keep the genetic purity of those introduced strains, they should be properly maintained in isolated places.

  10. Utilization of laserpuncture induction as spawning stimulation in catfish (Clarias spp. crossbreeding toward egg quality

    Directory of Open Access Journals (Sweden)

    Pungky S.W. Kusuma

    2015-12-01

    Full Text Available The induction of laserpuncture on the reproductive acupoint of catfish can accelerate gonadotropin hormone formation from the pituitary especially gonadotropin II (GTH-II which has a role in the final stage of oocyte maturation, ovulation and spawning stimulation. The purpose of this study is to evaluate the effects of laserpuncture induction toward the egg quality from crossbreeding catfish male var. Paiton and female var. Sangkuriang. The egg quality was measured by the following parameters: fertilization rate (FR, egg hatching rate (HR, and larvae survival rate (SR. The research treatments were conducted using two levels along with eight repetitions. The results show that the crossbreed catfish using laserpuncture induction affected the parameters by increasing the mean value of fertilization rate, egg hatching rate and larvae survival rate significantly (P < 0.05 compared with mean value of fish without induction. This study concluded that laserpuncture induction on the crossbreeding between broodstock of male catfish var. Paiton and female var. Sangkuriang will increase FR, HR and SR.

  11. Gender nor sex hormones alter the disease susceptibility of channel catfish to Edwardsiella ictaluri

    Science.gov (United States)

    The use of monosex populations for aquaculture is becoming widely used for several species. Monosex populations are not in wide use in the catfish industry but techniques to develop all male populations have been developed. These studies were conducted to determine if there were any differences be...

  12. Zinc effects on the embryos and larvae of the sharptooth catfish, Claias gariepinus (Burchell, 1822)

    CSIR Research Space (South Africa)

    Viljoen, A

    2003-05-01

    Full Text Available The aim of this study was to assess the sensitivity of early life stages of Sharp tooth catfish, Clarias gariepinus (Burchell), to determine which stage to use during effluent assessment. The target water quality of zinc was used ranging from 0...

  13. Dietary copper effects survival of channel catfish challenged with Flavobacterium columnare

    Science.gov (United States)

    Columnaris disease is one of the most important bacterial diseases of channel catfish, Ictalurus punctatus, commercially grown in the US. Copper sulfate (CuSO4) has also been shown to be both therapeutic and prophylactic as a water treatment for columnaris disease. As copper is an essential dietar...

  14. Governance Strategies and Welfare Effects: Vertical Integration and Contracts in the Catfish Sector in Vietnam

    DEFF Research Database (Denmark)

    Trifkovic, Neda

    2014-01-01

    Using an original dataset from the Vietnamese catfish sector, we study the impact of vertical coordination options on household welfare and the implications of different stages of vertical coordination for the success of the whole sector. The welfare gain from contract farming and employment on p...

  15. The Case Against Using Organic Fertilizers in Channel Catfish Ictalurus punctatus Nursery Ponds

    Science.gov (United States)

    This paper reviews the assumed advantages and disadvantages of organic fertilizers and presents the case that the risks outweigh the benefits for channel catfish, Ictalurus punctatus, nursery pond fertilization. Under certain conditions, organic fertilizers may be beneficial to provide forage for z...

  16. A dynamic simulation model for growth of the African catfish, Clarias gariepinus (Burchell 1822)

    NARCIS (Netherlands)

    Machiels, M.A.M.

    1987-01-01

    In the early '70 it was tried to identify new fish species for aquaculture in Africa. Amongst the most promising candidates was the African catfish. Clariasgariepinus (Burchell 1822). It is an omnivorous fish. which means a wide feeding spectrum. The

  17. Comparison of phytoplankton communities in catfish split-pond aquaculture systems with conventional ponds

    Science.gov (United States)

    There has been a growing interest and use of variations of partitioned aquaculture systems (PAS) in recent years by the southeastern United States of America farmed catfish industry. Split-pond systems, one type of PAS, are designed to better manage fish waste byproducts (e.g., ammonia) and dissolv...

  18. Scanning electron microscopy of Salmonella biofilms on various food-contact surfaces in catfish mucus

    Science.gov (United States)

    The objective of this study was to determine the growth and survival of Salmonella enterica in the presence of high and low concentrations (375 µg/ml and 15 µg/ml) of catfish mucus extract at 10 °C and 22 °C for 63 days. The second objective of this study was to investigate the biofilm formation of ...

  19. Developing a low cortisol responsive line of channel catfish, Ictalurus punctatus

    Science.gov (United States)

    Nagaraj G. Chatakondi and Brian C. Peterson USDA ARS Warmwater Aquaculture Research Unit, 141 Experiment Station Road, Stoneville, MS 38776. nagaraj.chatakondi@ars.usda.gov Channel catfish, Ictalurus punctatus is the most important farm-raised aquacultured species in the USA. Stressors in aquacultu...

  20. Plasma cortisol stress response in channel catfish, Ictalurus punctatus influences susceptibility to Edwardeseilla ictaluri

    Science.gov (United States)

    Cortisol is a primary stress hormone in fish as its plasma variations correlate with the occurrence of various stressful situations. Past studies have demonstrated that fish subjected to handling stress or poor water quality had a reduced ability to resist pathogens. Channel catfish fingerlings th...

  1. Efficacy of Sodium Carbonate Peroxyhydrate as A Catfish Egg Disinfectant and Comparison to Hydrogen Peroxide

    Science.gov (United States)

    Two experiments were conducted to evaluate the efficacy of sodium carbonate peroxyhydrate (SCP) for improving channel catfish Ictalurus punctatus hatching success when used as a prophylactic chemotherapeutant during egg incubation. In the first experiment, efficacy of SCP was evaluated in 379-L, al...

  2. PCDD/Fs, PCBs, and PBDEs in catfish from U.S. Commerce

    Science.gov (United States)

    The U.S. Department of Agriculture (USDA) is responsible for the safety of meat, poultry, egg products, and farm-raised catfish marketed in the United States. As such the USDA conducts statistical surveys to determine chemical residue concentrations in these domestic products. Little background data...

  3. Acute toxicity of cassava mill effluent to the African catfish fingerlings ...

    African Journals Online (AJOL)

    A bioassay test was performed on the toxic effect of Cassava Mill Effluent to the African Catfish - Heteroclarias Hybrid of Heterobranchus bidorsalis (Male) and Clarias gariepinus (Female). The 96-h LC50 was determined as 50. 12 mgl -1. Exposed fish became darker in colour and showed signs of respiratory distress, ...

  4. The effects of brood stock size on the economy of catfish ( Clarias ...

    African Journals Online (AJOL)

    Eighty nine ripe female brooders of the catfish, Clarias anguillaris (Body weight. range: 150 1,200 g) were induced to spawn by hormone (Ovaprim®) induced natural spawning technique over a period of 10 weeks. Matching ripe males were used for pairing the females at the ratio of two males to a female. Six ranges of ...

  5. Bagrichthys vaillantii (Popta, 1906), a valid species of bagrid catfish from eastern Borneo (Teleostei: Siluriformes)

    NARCIS (Netherlands)

    Ng, H.H.

    2000-01-01

    Bagrichthys vaillantii (Popta, 1906), a species of bagrid catfish previously considered a junior synonym of B. macracanthus Bleeker, 1854, is found to be a valid species distinct from the latter. It can be differentiated from B. macracanthus in having a shorter dorsal spine, smaller eye and steeper

  6. Toxicity of organophosphorus pesticide sumithion on larval stages of stinging catfish Heteropneustes fossilis

    NARCIS (Netherlands)

    Shahjahan, M.; Kabir, M.F.; Sumon, Kizar Ahmed; Bhowmik, Lipi Rani; Rashid, Harunur

    2017-01-01

    Sumithion is widely used to control brittle in paddy fields and tiger bug in fish larval rearing ponds. The objective of this study was to elucidate the toxic effects of sumithion on larval stages of stinging catfish Heteropneustes fossilis. Larvae were exposed to two concentrations (150 and 250

  7. The use of trilene- as anesthesia and triquilizer for catfish Clarias ...

    African Journals Online (AJOL)

    The culture of the African catfish Clarias gariepinus is developing fast that more easier way of handling bigger fish is required both in the transportation and hypophysation/breeding. Such inputs like tranquilizer/anesthesia would help to reduce handling stress thereby reduce injury and death after operation. Trilene ...

  8. Chemical alarm cues in juvenile African catfish, Clarias gariepinus Burchell: A potential stressor in aquaculture?

    NARCIS (Netherlands)

    Nieuwegiessen, van de P.G.; Zhao, H.; Verreth, J.A.J.; Schrama, J.W.

    2009-01-01

    Previous studies on the effects of stocking density on the behaviour of African catfish have shown that at low densities, especially directly after restocking of tanks, increased aggression might occur. This aggression may directly affect the welfare of the fish. In addition, the resulting skin

  9. The use of cold shock in inducing triploidy in African mud catfish ...

    African Journals Online (AJOL)

    study was conducted to induce triploidy in African mud catfish Clarias gariepinus using cold shock. The fertilized eggs were exposed at one temperature regime of 0°C with varied shock treatments of 0, 15, 25 and 30 min. Some 3 min after fertilization, the success of triploidy was determined by the presence or absence of ...

  10. Growth Performance of Catfish (Clarias Gariepinus Burchell, 1822) Cultured in High Density on the Biofloc System

    Science.gov (United States)

    Basuki, Fajar; Yuniarti, Tristiana; Harwanto, Dicky; Susilowati, Titik

    2018-02-01

    Start your The fish farmers and researchers have made efforts to increase catfish production through high levels of dispersal and application of biofloc systems. This system, in addition to increase production, can also predict when the harvest is done, control the quality of water causing death so that the Survival Rate (SR) is high. The purpose of this study is to determine the growth performance and predict the harvest weight and catfish survival rate (SR) that are cultured with different high density on the biofloc system. The research method used is experimental with test fish in the form of catfish seeds weighing of 8 ± 0.2 gram/fish with each density as treatment: P1. Density of 1,100 fish/m3; P2. Density of 1,200 fish/m3 and P3. Density of 1,300 fish/m3. Pond: The pond used is made of 3m in diameter of tarpaulin, equipped with a 6mm diameter of iron frame. Total volume of water in each pond is 3 m3. Catfish seeds have been adapted and put into the pond at random with the length of cultivation period is 4 weeks. Growth performance analysis uses the SPSS regression statistic series 20. The research results showed that the growth performance for 4 weeks showed that P1 generates the regression equation Y1 = 6.46 +7.81 X with r2 = 0.971 means that if the harvest is done at week 12th then the weight of catfish has reached 100 grams; P2 generates the regression equation Y2 = 6.53 + 6.0 X den r2 = 0.969 means if the harvest is done at week 12th then the weight of new catfish has reached 78.53 grams and P3 generates the regression equation Y3 = 6.71 + 5.94 X with r2 = 0.976 means if harvest is done on week 12th then the weight of new catfish has reached 77.99 grams. Survival rate at P1 = 97.73%; P2 = 95.91% and P3 = 94.55% while the oxygenated water quality is more than 3.5 ppm; pH ranged from 6.7-7 and ammonia content at week 3 is P1 = 0.04 mg/l; P2 = 0.13 mg/l and P3 = 0.27 mg/l.

  11. Chlorella vulgaris as Protein Source in the Diets of African Catfish Clarias gariepinus

    Directory of Open Access Journals (Sweden)

    Uchechukwu D. Enyidi

    2017-10-01

    Full Text Available Plant proteins substitutes of fishmeal in aquafeed are usually lacking in some essential amino acids and fatty acids. The microalgae Chlorella vulgaris has good-quality protein with amino acids rich in methionine, lysine and alanine. Four novel diets having C. vulgaris as the main source of protein were produced for African catfish Clarias gariepinus with an initial average weight of 1.09 ± 0.05 g. The diets were labeled Feed 1 (F1 to feed 4 (F4. The treatment diets were included 25% (F1, 15% (F2, 5% (F3 and 0% (F4 green algae meal. The basal ingredients of the feed were corn (maize included as F1, 40%, F2, 43%, F3, 53% and F4, 43%; and millet meal, which varied in F1 as 23%, F2, 30%, F3, 30% and F4, 30%. The ingredients were preconditioned at 110 °C and pelleted. Post-fingerling African catfish were stocked at 10 fish per aquarium. There were three replicate aquariums for each feed type and the fish were fed for 60 d. The specific growth rate was best for the catfish fed with 25% C. vulgaris diet 7.86 ± 0% day−1, and worst at 6.77 ± 0.07% day−1 for the control group F4, 0% algal meal. The food conversion ratio (FCR was lowest (1.88 ± 0.02 for 25% algal meal diet (F1 and highest (2.98 ± 0.01 for the 0% algal meal diet F4. Similarly, catfish had average weight gain of 121.02 ± 0.04 g for those fed with F1 compared to 62.50 ± 0.0 g for those fed with 0% algae F4. Protein efficiency ratio was highest for the F1-fed fish (2.46 ± 0.22 and lowest for those fed with F4 (2.02 ± 0.09. The hepatosomatic index was lowest for F1-fed fish (1.48 ± 0.01 and highest for catfish fed with F4 (2.50 ± 0.59. Based on the results, C. vulgaris is a good protein source for African catfish and can also substitute fishmeal in the catfish diets.

  12. Air breathing and aquatic gas exchange during hypoxia in armoured catfish.

    Science.gov (United States)

    Scott, Graham R; Matey, Victoria; Mendoza, Julie-Anne; Gilmour, Kathleen M; Perry, Steve F; Almeida-Val, Vera M F; Val, Adalberto L

    2017-01-01

    Air breathing in fish is commonly believed to have arisen as an adaptation to aquatic hypoxia. The effectiveness of air breathing for tissue O 2 supply depends on the ability to avoid O 2 loss as oxygenated blood from the air-breathing organ passes through the gills. Here, we evaluated whether the armoured catfish (Hypostomus aff. pyreneusi)-a facultative air breather-can avoid branchial O 2 loss while air breathing in aquatic hypoxia, and we measured various other respiratory and metabolic traits important for O 2 supply and utilization. Fish were instrumented with opercular catheters to measure the O 2 tension (PO 2 ) of expired water, and air breathing and aquatic respiration were measured during progressive stepwise hypoxia in the water. Armoured catfish exhibited relatively low rates of O 2 consumption and gill ventilation, and gill ventilation increased in hypoxia due primarily to increases in ventilatory stroke volume. Armoured catfish began air breathing at a water PO 2 of 2.5 kPa, and both air-breathing frequency and hypoxia tolerance (as reflected by PO 2 at loss of equilibrium, LOE) was greater in individuals with a larger body mass. Branchial O 2 loss, as reflected by higher PO 2 in expired than in inspired water, was observed in a minority (4/11) of individuals as water PO 2 approached that at LOE. Armoured catfish also exhibited a gill morphology characterized by short filaments bearing short fused lamellae, large interlamellar cell masses, low surface area, and a thick epithelium that increased water-to-blood diffusion distance. Armoured catfish had a relatively low blood-O 2 binding affinity when sampled in normoxia (P 50 of 3.1 kPa at pH 7.4), but were able to rapidly increase binding affinity during progressive hypoxia exposure (to a P 50 of 1.8 kPa). Armoured catfish also had low activities of several metabolic enzymes in white muscle, liver, and brain. Therefore, low rates of metabolism and gill ventilation, and a reduction in branchial gas

  13. FEATURES OF DEVELOPMENT OF SOME ORGANS OF AIRBREATHING CATFISH (CLARIIDAE IN EARLY ONTOGENESIS

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    A. V. Pirog

    2015-01-01

    Full Text Available Aim. Airbreathing catfish (CLARIIDAE - is one of the most promising specie of artificial breeding. However, for the reproduction of this specie it is required to conduct appropriate studies. In particular, it is necessary to review the features of morphogenesis during early ontogeny of this type of fish. We investigated prelarvae of airbreathing catfish (CLARIIBAE in 2,3 and 4 days of life. In the embryos, the spinal cord was formed on the 2nd day of life. At this stage, organs of vision were sufficiently developed. On the 4th day, we found hyaline cartilage at the base of the gill arch, arcs were covered with gill filaments; we also discovered fully formed mesonephros, renal corpuscles, renal tubules and intratubular liquid in the kidneys. The heart of the embryo consisted of atrium and voluminous ventricle.Methods. The research of development of airbreathing catfish in early ontogenesis has been carried out on the basis of "RENTOP Agro-5" Ltd. under the conditions of RAS in the Krasnodar region in the spring – summer period of years 2013-14. For morphological analysis we used serial histological sections on prelarvae of 40 airbreathing catfish at age 2, 3 and 4 days developing normally and atypically. The research combines some complex biological methods: ichthyologic, histological, physiological. Materials for the research were handled with the methods of classical histology. Viewing and imaging of the sections were made using Mikmed 6 microscope with a digital camera for imaging and computer analysis.Results. The finding of the study has been the analysis of the morphological structure of airbreathing catfish during early ontogeny, which can contribute to improving the biotechnology of breeding of airbreathing catfish under RAS.Main conclusions. First, in the early ontogenesis of airbreathing catfish we have discovered heterochrony in the development of basic systems: rapidly developing central nervous system and the digestive system

  14. Evaluation of a modified pH-shift process to reduce 2-methylisoborneol and geosmin in spiked catfish and produce a consumer acceptable fried catfish nugget-like product.

    Science.gov (United States)

    Brown, T M; Cerruto-Noya, C A; Schrader, K K; Kleinholz, C W; DeWitt, C A Mireles

    2012-10-01

    Muddy and/or musty off-flavors in farmed-raised catfish occur as a result of the absorption of geosmin (GEO) and 2-methylisoborneol (MIB), compounds produced by algae. Previous research suggests the acid pH-shift method may be able to reduce off-flavors to produce a consumer acceptable product. The objective of this research was to evaluate application of the acid pH-shift method using a shaker sieve for protein recovery and to evaluate consumer acceptability of a resultant batter-coated fried nugget-like catfish product. Farm-raised catfish were either allowed to depurate (control) or treated with 1 ppb GEO or MIB. Fillets from each replicate were collected and ground and treated by the acid pH-shift process. Samples from all treatments and replicates were evaluated for residual GEO and MIB. In addition, batter-coated fried catfish samples were prepared for a consumer sensory evaluation. Results demonstrated that the pH-shift process decreased moisture, ash, and collagen content of catfish fillet tissue (P evaluated for removing off-flavors from catfish. Difficulties were encountered with regard to protein recovery using the sieve and suggestions are made to, perhaps, make the process more applicable for a sieve-based recovery step. The process as described reduced off-flavors, but only 2-fold suggesting the process would work best on catfish near or just over off-flavor thresholds. Results also indicated the pH-shift process could be used to improve texture of a fried catfish product designed to be similar to chicken nuggets. © 2012 Institute of Food Technologists®

  15. 6-Desaturase-Like Encoding Gene Introduction in Catfish (Clarias gariepinus

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    Anny Hary Ayu

    2017-11-01

    Full Text Available African catfish (Clarias gariepinus is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce F0 transgenic catfish carrying masou salmon Δ6-desaturase-like (OmΔ6FAD gene. The Δ6-desaturase enzyme is involved in highly unsaturated fatty acid biosynthesis. Transgenic catfish was produced by sperm-mediated gene transfer using electroporation method. In this study, as the first step, sperms were electroporated with three different OmΔ6FAD concentration (25, 50, and 100 µg mL-1 to have the highest sperm viability after electroporation (125 V/cm, pulse frequency 5 times, pulse length 30 millisecond, pulse interval 0.1 second. The highest sperm viability and sperm carrying OmΔ6FAD were obtain at 100 µg mL-1. This concentration was then used to produce F0 transgenic catfish in the second step. Sperm motility, sperm viability, fertilization rate, hatching rate, and larval survival at 14 days after hatching were the same as the controls (p>0.05. Genomic DNA was extracted from caudal fin and then used as template to identify transgenic F0 by PCR method using specific primer for OmΔ6FAD gene. The PCR result showed that 53.84% of F0 carried OmΔ6FAD gene. The result of fatty acid analysis showed that EPA and DHA contents of F0 transgenic fish and non-transgenic fish were similar.   Keywords: catfish, Δ6-desaturase-like gene, fatty acids, electroporation

  16. Feeding for larvae of catfish Pangasionodon sp. larvae in different ages

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    Muhammad Agus Suprayudi

    2015-05-01

    Full Text Available ABSTRACT Sludge worm (Tubifex sp. as natural feed on catfish (Pangasionodon sp. larvae rearing is available in limited amount especially during rainy season. It becomes a constraint factor for larvae rearing sector. This research was conducted to evaluate the appropriate initial age of catfish larvae to get artificial feed as sludge worm replacement. Evaluation was conducted on the growth and survival of catfish larvae in 14 days of culture. There were four treatments of feeding in triplicates i.e. larvae were given natural feed without artificial feed, given artificial feed started from d3, d6, and d9 with three replications. The results showed that larvae fed on artificial feed on d3 had the lowest growth compared to the other treatments, whereas the survival was not significantly different (P>0.05 among the treatments. As a conclusion, artificial feed could be used to replace natural feed for catfish larvae started at the age of nine days. Keywords: sludge worm, catfish larvae, artificial feed  ABSTRAK Cacing sutra (Tubifex sp. tersedia dalam jumlah terbatas terutama pada musim penghujan sebagai pakan alami dalam usaha pembenihan ikan patin (Pangasionodon sp.. Ini menjadi kendala dalam usaha pembenihan. Penelitian ini dilakukan untuk mengevaluasi umur larva ikan patin yang tepat untuk mulai diberi pakan buatan menggantikan cacing sutra. Evaluasi dilakukan pada pertumbuhan dan kelangsungan hidup larva ikan patin umur 14 hari. Selama pemeliharaan, larva diberi pakan dengan empat perlakuan; pemberian pakan alami tanpa pakan buatan, pemberian pakan buatan mulai d3, d6, dan d9 dengan tiga ulangan untuk masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan pemberian pakan buatan mulai d3 memiliki pertumbuhan panjang yang terkecil dibandingkan perlakuan lain, sedangkan tingkat kelangsungan hidup larva tidak berbeda nyata (P>0,05 antarperlakuan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pakan buatan dapat digunakan

  17. Development and performance evaluation of air fine bubbles on water quality of thai catfish rearing

    Science.gov (United States)

    Subhan, Ujang; Muthukannan, Vanitha; Azhary, Sundoro Yoga; Mulhadi, Muhammad Fakhri; Rochima, Emma; Panatarani, Camellia; Joni, I. Made

    2018-02-01

    The efficiency and productivity of aquaculture strongly depends on the development of advanced technology for water quality management system. The most important factor for the success of intensive aquaculture system is controlling the water quality of fish rearing media. This paper reports the design of fine bubbles (FBs) generator and performance evaluation of the system to improve water quality in thai catfish media (10 g/ind) with density (16.66 ind./L). The FBs generator was designed to control the size distribution of bubble by controlling its air flow rate entry to the mixing chamber of the generator. The performance of the system was evaluated based on the produced debit, dissolved oxygen rate and ammonia content in the catfish medium. The size distribution was observed by using a high speed camera image followed by processing using ImageJ. freeware application. The results show that air flow rate 0.05 L/min and 0.1 L/min received average bubble size of 29 µm and 31 µm respectively. The generator produced bubbles with capacity of 6 L/min and dissolved oxygen rate 0.2 ppm/min/L. The obtained DO growth was 0.455 ppm/second/L while the average decay rate was 0.20 ppm/second/L. (0.011/0.005 fold). In contrast, the recieved DO growth rate is faster compared to the DO consumption rate of the Thai catfish. This results indicated that the potential application of FBs enhanced the density of thai catfish seed rearing. In addition, ammonia can be reduced at 0.0358 ppm/hour/L and it is also observed that the inhibition of bacterial growth of air FBs is postive to Aeromonas hydrophila bacteria compared to the negative control. It is concluded that as-developed FBs system can be potentially applied for intensive thai catfish culture and expected to improve the feeding efficiency rate.

  18. Portable canvas tanks for culture of hybrid catfish (Clarias gariepinus x Clarias macrocephalus) by small-scale farmers in Malaysia

    OpenAIRE

    Yaakob, W.A.A.; Ali, A.B.

    1994-01-01

    This paper describes the trials made with a simple portable canvas-tarpaulin tank system developed at the University Sains Malaysia for culture of hybrid catfish (Clarias gariepinus x Clarias macrocephalus) by Malaysia small scale farmers.

  19. Empowering Farmer’s Wife Makes Catfish Feed (Pellet) in Nagari Limau Gadang Pesisir Selatan District, Indonesia

    Science.gov (United States)

    Armen, A.

    2018-04-01

    The life of Nagari Limau Gadang, Pesisir Selatan District, West Sumatra, Indonesia is poor. They conduct economic activities in Kerinci Seblat National Park (TNKS), so that TNKS become damaged. Catfish farming can serve as the main effort to increase family income. Economically catfish farming is very profitable. Catfish farming is not difficult and can be done by farmers who do not usually cultivate freshwater fish. The right family member is empowered to improve the family economy through the cultivation of catfish is the farmer's wife. Farmers' wives in Nagari Limau Gadang tend not to improve the family economically, they play the role of taking care of children, doing housework and sometimes delivering food to the fields and to the fields. How to research, selected 20 poor farmer’s wife, then trained to make ponds and pellets. The pool made are the main pond, spawning pond, nursery pool and pond enlargement. Pellet made from raw trash fish, quail feces, fine bran and cassava. The pellet formulation is made of 4 kinds. Each formulation produces a quality pellet. Based on laboratory test produced pellet contains very good nutrition for enlargement catfish. The nutritional range of protein is 21,79%-34,60%, fat is 1,95%-2,32% and carbohydrate is 26,55%-39,65%. The goal to be achieved in this research is for the wife of farmers can contribute to improve the family economy. Specific targets to be achieved in this study is the wife of skilled farmers cultivate catfish.

  20. Effects of dietary sweet potato leaf meal on the growth, non-specific immune responses, total phenols and antioxidant capacity in channel catfish (Ictalurus punctatus).

    Science.gov (United States)

    Lochmann, Rebecca T; Islam, Shahidul; Phillips, Harold; Adam, Zelalem; Everette, Jace

    2013-04-01

    Traditional energy sources in catfish diets have become costly, and economical alternatives are needed. Sweet potato leaves are underutilised agricultural by-products that provide energy and substantial amounts of phenols, which affect animal and human health. There is little information on the effects of these compounds on catfish, or the capacity of catfish to accumulate dietary phenols. Catfish enriched with phenols have marketing potential as functional foods. This study investigated the effects of diets with sweet potato leaf meal (SPLM) on growth performance, health and total phenolic compounds in catfish. SPLM was substituted for wheat middlings in three diets fed to groups of juvenile catfish for 10 weeks. Weight gain, feed conversion, survival, alternative complement activity and lysozyme activity were similar among diets. Haematocrit was lower in fish fed diets with SPLM, but within the normal range. Total phenols and antioxidant capacity in the whole body were similar among treatments. SPLM was an effective energy source for catfish up to the maximum level tested (230 g kg(-1) diet). SPLM did not enhance total phenols in catfish, but there were no apparent antinutritional effects of the meal on catfish growth, health or survival. © 2012 Society of Chemical Industry.

  1. KANDUNGAN ASAM LEMAK DAN KARAKTERISTIK FISIKO-KIMIA MINYAK IKAN LELE DAN MINYAK IKAN LELE TERFERMENTASI (FATTY ACID CONTENTS AND PHYSICO-CHEMICAL CHARACTERISTICS OF CATFISH OIL AND FERMENTED CATFISH OIL

    Directory of Open Access Journals (Sweden)

    Iskari Ngadiarti

    2013-06-01

    Full Text Available ABSTRACT Catfish oil and fermented catfish oil have not been developed and commercially produced as catfish derived products. Various processing of catfish oil will change both the composition and physico-chemical characteristics. The objective of this study was to identify the composition of fatty acids and physico-chemical characteristics of both catfish oil (MIL and fermented catfish oil (MILT. Results showed that fatty acid composition of catfish oil was MUFA (36.12%> PUFA> (32.43%> SFA (31.45%, while the composition of fermented catfish oil was MUFA (42.96%> SFA (42.32%> PUFA (15.39%. The fermentation process with lactic acid bacteria increased the content of stearic acid but decreased the content of linoleic acid and linolenic acid. Physical and chemical characteristics of MIL and MILT were almost similar, they indicated oxidation process, based on melting point, viscosity, and thiobarbituric acid values. Therefore, in the production of catfish oil or fermented cat fish oil, it is necessary to add spices and/or nutrient as antioxidants sources. Keywords: catfish oil, PUFA, MUFA, SFA and CLA  ABSTRAK Minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT sebagai produk turunan dari ikan lele masih belum banyak dikembangkan dan diproduksi secara komersial. Berbagai proses pengolahan minyak dapat mengubah komposisi dan karakteristik fisiko-kimia minyak ikan lele. Tujuan dari penelitian ini adalah untuk mengidentifikasi komposisi asam lemak dan karakteristik fisiko-kimia dari minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT. Komposisi asam lemak pada MIL secara berurutan adalah MUFA (36,12% > PUFA (32,43% > SFA (31,45%, sedangkan setelah difermentasi menjadi MILT terjadi perubahan yaitu MUFA (42,96% > SFA (42,32% > PUFA (15,39%. Jenis asam lemak jenuh yang mengalami peningkatan  pada MILT adalah asam lemak stearat, sedangkan asam lemak tidak jenuh ganda yang mengalami penurunan adalah asam linoleat dan linolenat yang

  2. Novel transcripts of the estrogen receptor α gene in channel catfish

    Science.gov (United States)

    Patino, Reynaldo; Xia, Zhenfang; Gale, William L.; Wu, Chunfa; Maule, Alec G.; Chang, Xiaotian

    2000-01-01

    Complementary DNA libraries from liver and ovary of an immature female channel catfish were screened with a homologous ERα cDNA probe. The hepatic library yielded two new channel catfish ER cDNAs that encode N-terminal ERα variants of different sizes. Relative to the catfish ERα (medium size; 581 residues) previously reported, these new cDNAs encode Long-ERα (36 residues longer) and Short-ERα (389 residues shorter). The 5′-end of Long-ERα cDNA is identical to that of Medium-ERα but has an additional 503-bp segment with an upstream, in-frame translation-start codon. Recombinant Long-ERα binds estrogen with high affinity (Kd = 3.4 nM), similar to that previously reported for Medium-ERα but lower than reported for catfish ERβ. Short-ERα cDNA encodes a protein that lacks most of the receptor protein and does not bind estrogen. Northern hybridization confirmed the existence of multiple hepatic ERα RNAs that include the size range of the ERα cDNAs obtained from the libraries as well as additional sizes. Using primers for RT-PCR that target locations internal to the protein-coding sequence, we also established the presence of several ERα cDNA variants with in-frame insertions in the ligand-binding and DNA-binding domains and in-frame or out-of-frame deletions in the ligand-binding domain. These internal variants showed patterns of expression that differed between the ovary and liver. Further, the ovarian library yielded a full-length, ERα antisense cDNA containing a poly(A) signal and tail. A limited survey of histological preparations from juvenile catfish by in situ hybridization using directionally synthesized cRNA probes also suggested the expression of ERα antisense RNA in a tissue-specific manner. In conclusion, channel catfish seemingly have three broad classes of ERα mRNA variants: those encoding N-terminal truncated variants, those encoding internal variants (including C-terminal truncated variants), and antisense mRNA. The sense variants may

  3. Evaluation of integrated sludge worm and catfish farming with biofloc system

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    Dedi Pardiansyah

    2015-05-01

    Full Text Available ABSTRACTCurrently, supply of sludge worm (Tubifex sp. merely came from wild, so that it could not meet demand for fish hatchery. Additionally, harvest from the wild did not have a quality assurance, due to the possibility of sludge worm becoming an agent of disease. This study was conducted to evaluate the production of sludge worm culture by utilizing catfish culture waste (Clarias sp. in bioflok system. In this system, water from catfish culture media flowed into sludge worm culture media using recirculation systems. This study used a completely randomized design with four treatments and two replications, in which treatment A (addition of catfish culture waste from intensive system, treatment B (addition of catfish culture waste from biofloc system, treatment C (the addition of fermented chicken manure at the beginning of experiment as negative control, and treatment D (addition of fermented chicken manure at the beginning of experiment and then every five days as positive control. The results showed that the highest production was obtained by treatment B at biomass growth of 0.97 kg/m2 and sludge worm density of 388.000 individu/m2. Keywords: sludge worm, biofloc, chicken manure, catfish, catfish culture waste  ABSTRAK Saat ini pasokan cacing sutra (Tubifex sp. hanya berasal dari alam, sehingga belum mencukupi permintaan untuk kegiatan pembenihan ikan. Selain itu, hasil tangkapan dari alam tidak memiliki jaminan kualitas, karena cacing sutra dapat menjadi agen penyakit. Penelitian ini dilakukan untuk mengevaluasi hasil produksi budidaya cacing sutra dengan memanfaatkan limbah budidaya ikan lele (Clarias sp. sistem bioflok. Pada sistem ini, air dari media budidaya ikan lele dialirkan ke media pemeliharaan cacing sutra menggunakan sistem resirkulasi. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan dua ulangan, yaitu perlakuan A (pemberian limbah ikan lele sistem intensif, perlakuan B (pemberian limbah ikan lele

  4. Detection of Off-Flavor in Catfish Using a Conducting Polymer Electronic-Nose Technology

    Science.gov (United States)

    Wilson, Alphus D.; Oberle, Charisse S.; Oberle, Daniel F.

    2013-01-01

    The Aromascan A32S conducting polymer electronic nose was evaluated for the capability of detecting the presence of off-flavor malodorous compounds in catfish meat fillets to assess meat quality for potential merchantability. Sensor array outputs indicated that the aroma profiles of good-flavor (on-flavor) and off-flavor fillets were strongly different as confirmed by a Principal Component Analysis (PCA) and a Quality Factor value (QF > 7.9) indicating a significant difference at (P 90%) and with relatively low rates (≤5%) of unknown or indecisive determinations in three trials. This A32S e-nose instrument also was capable of detecting the incidence of mild off-flavor in fillets at levels lower than the threshold of human olfactory detection. Potential applications of e-nose technologies for pre- and post-harvest management of production and meat-quality downgrade problems associated with catfish off-flavor are discussed. PMID:24287526

  5. Catfish thrombocytes express an integrin-like CD41/CD61 complex.

    Science.gov (United States)

    Passer, B J; Chen, C H; Miller, N W; Cooper, M D

    1997-08-01

    A thrombocyte-specific antigen was identified in two closely related catfish, Ictalurus punctatus and Ictalurus furcatus, by monoclonal antibodies 4-20 and 7-2. The antibodies immunoprecipitate two noncovalently associated glycoprotein chains of Mr 180,000 and Mr 95,000. Under reducing conditions the Mr 180,000 chain is resolved into Mr 150,000 and 32,000 subcomponents. Analysis of N-terminal amino acid sequences indicates homology of the Mr 95,000 chain with the beta3 integrin subunit and homology of the Mr 150,000 chain with the alphaIIb integrin subunit. These antibodies induce catfish thrombocyte aggregation and alteration of cell shape. The data indicate conservation of the megakaryocyte/platelet-restricted CD41/CD61 complex in bony fish.

  6. Survival and behavior of silver catfish, Rhamdia quelen, submitted to antibiotics and sodium chloride treatments

    Directory of Open Access Journals (Sweden)

    Andrade Luciana Segura de

    2006-01-01

    Full Text Available In order to evaluate the use of antibiotics and NaCl on the behavior and survival of silver catfish, Rhamdia quelen, infested by Ichthyophthirius multifiliis and infected with Aeromonas hydrophila, juveniles were treated with chloramphenicol, chloramphenicol + salt, oxytetracycline, oxytetracycline + salt and water alone (control. Fish survival in the treatments with chloramphenicol + salt and oxytetracycline + salt was significantly higher than in the other treatments. The treatment with chloramphenicol presented higher survival than the treatment with oxytetracycline and both showed significantly higher survival than control. Swimming activity was higher in the fish treated with antibiotics and salt compared to control fish. A combination of the studied antibiotics plus salt is more effective to treat both A. hydrophila infection and I. multifiliis infestation in silver catfish, but since the use of chloramphenicol is not allowed in Brazil, oxytetracycline plus salt seems to be the best treatment option.

  7. Production of Generation-2 Mekong giant catfish (Pangasinodon gigas cultured with Spirulina sp.

    Directory of Open Access Journals (Sweden)

    Kriangsak Meng-umphan

    2008-11-01

    Full Text Available The purpose of this study is to evaluate the treatment of Spirulina-supplemented pellet feed to 5-year-old F1 groups of Mekong giant catfish (Pangasinodon gigas from the brood stock and intended for use as breeders. The effects on their growth and maturation when cultured in an earthen pond were observed. Results revealed that, compared to control, there was more gain in weight while the feed conversion ratio was lower. The number of red blood cells was also higher while that of white blood cells was lower, compared to control. Out of 18 treated fish (9 males and 9 females, 6 males and 2 females gave sperms and eggs while none from control group did. It was concluded that Spirulina supplemented in pellet feed can improve growth and maturation performance to the brood stock of Mekong giant catfish.

  8. Electric Organ Discharges of Mormyrid Fish as a Possible Cue for Predatory Catfish

    Science.gov (United States)

    Hanika, S.; Kramer, B.

    During reproductive migration the electroreceptive African sharptooth catfish, Clarias gariepinus (Siluriformes), preys mainly on a weakly electric fish, the bulldog Marcusenius macrolepidotus (Mormyridae; Merron 1993). This is puzzling because the electric organ discharges of known Marcusenius species are pulses of a duration (system (optimum sensitivity, 10-30Hz Peters and Bretschneider 1981). On the recent discovery that M. macrolepidotus males emit discharges lasting approximately ten times longer than those of females (Kramer 1997a) we determined behavioral thresholds for discharges of both sexes, using synthetic playbacks of field-recorded discharges. C. gariepinus detected M. macrolepidotus male discharges down to a field gradient of 103μVpeak-peak/cm and up to a distance of 1.5m at natural field conditions. In contrast, thresholds for female discharges were not reached with our setup, and we presume the bulldogs eaten by catfish are predominantly male.

  9. Longitudinal patterns in flathead catfish relative abundance and length at age within a large river: Effects of an urban gradient

    Science.gov (United States)

    Paukert, C.P.; Makinster, A.S.

    2009-01-01

    We investigated the spatial variation of flathead catfish (Pylodictis olivaris) relative abundance and growth in the 274 km long Kansas River to determine if population dynamics of catfish are related to urbanization. Electrofishing was conducted at 462 random sites throughout the river in summer, 2005-2006 to collect fish. Relative abundance of age 1 fish (???200mm), subadult (>200-400mm) and adult fish (>400 mm) ranged from 0.34 to 14.67 fish h-1, mean length at age 1 was 165 (range: 128-195) mm total length (TL) and mean length at age 3 was 376 mm TL (range: 293-419mm TL). The proportion of land use within 200 m of the river edge was between 0 and 0.54 urban. River reaches with high relative abundance of age 1 flathead catfish had high relative abundance of subadult and adult catfish. River reaches with fast flathead catfish growth to age 1 had fast growth to age 3. High urban land use and riprap in the riparian area were evident in river reaches near the heavily populated Kansas City and Topeka, Kansas, USA. Reaches with increased number of log jams and islands had decreased riparian agriculture. Areas of low urbanization had faster flathead catfish growth (r = 0.67, p = 0.005). Relative abundance of flathead catfish was higher in more agricultural areas (r = -0.57, p = 0.02). Changes in land use in riverine environments may alter population dynamics of a fish species within a river. Spatial differences in population dynamics need to be considered when evaluating riverine fish populations. Published in 2008 by John Wiley & Sons Ltd.

  10. Distribution and relative abundance of the marine catfish (Siluriformes, Ariidae) in Sepetiba Bay, Rio de Janeiro

    OpenAIRE

    Azevedo, Márcia Cristina Costa de; Araújo, Francisco Gerson; Cruz Filho, Antônio Gomes da; Santos, Alexandre Clístenes de Alcântara

    1998-01-01

    Marine catfish (Ariidae) are abundant resources in otter trawl fisheries carried out at Sepetiba Bay, Rio de Janeiro (Lat. 22º54, 23º04'S; Long. 43º34 44º10'W). Relative abundance and distribution were assessed, based in 158 fishing sampling at seven sites in the Bay, between July-1993 e June-1996. Five species were recorded in the following abundance rank order: Genidens genidens (Valenciennes, 1839), Caihorops spixii (Agassiz,1829), Sciadeichthys lunisculis (Valenciennes, 1840), Nelunia bar...

  11. Stress and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) in a Coupled Aquaponic System

    OpenAIRE

    Björn Baßmann; Matthias Brenner; Harry W. Palm

    2017-01-01

    African catfish (Clarias gariepinus) were investigated for physiological stress parameters to assess the influence of different cultivation techniques on fish welfare. The fish were reared in two conceptually identical recirculating aquaculture systems (RAS) constructed in a greenhouse. One system was combined with a floating raft hydroponic unit to culture cucumbers (Cucumis sativus) only using the process water. The second system remained without the plant cultivation unit and functioned as...

  12. Revision of Wenyonia Woodland, 1923 (Cestoda: Caryophyllidea) from catfishes (Siluriformes) in Africa

    Czech Academy of Sciences Publication Activity Database

    Schaeffner, Bjoern C.; Jirků, Miloslav; Mahmoud, Z. N.; Scholz, Tomáš

    2011-01-01

    Roč. 79, č. 2 (2011), s. 83-107 ISSN 0165-5752 R&D Projects: GA ČR GA524/08/0885; GA AV ČR KJB600960813; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Wenyonia * catfishes * Africa * key to the identification * phylogenetic analysis Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.250, year: 2011

  13. Burrowing by Sailfin Catfish (Pterygoplichthys sp.): A Potential Cause of Erosion in Disturbed Environments

    Science.gov (United States)

    2014-03-01

    originates at a large levee, which was built to create a water retention (to control excess water) and water bird management area. The levee and canal...each sample was dried in stainless steel trays in a 60 °C convection oven . Two methods of quantitative analysis were performed to determine the...ERDC/TN ANSRP-14-1 March 2014 Approved for public release; distribution is unlimited. Burrowing by Sailfin Catfish (Pterygoplichthys sp.): A

  14. Reproductive potential of male catfish treated with gel extract of Aloe ...

    African Journals Online (AJOL)

    The reproductive potential of male catfish treated with gel extract of Aloe vera plant was studied using twelve male fish weighing 500-560g.The fish were divided into 3 groups; A, B and C with four fish in each group. Group A was treated with 2% Aloe vera gel while group B was treated with 3% and Group C the control was ...

  15. Effect of bait and gear type on channel catfish catch and turtle bycatch in a reservoir

    Science.gov (United States)

    Cartabiano, Evan C.; Stewart, David R.; Long, James M.

    2014-01-01

    Hoop nets have become the preferred gear choice to sample channel catfish Ictalurus punctatus but the degree of bycatch can be high, especially due to the incidental capture of aquatic turtles. While exclusion and escapement devices have been developed and evaluated, few have examined bait choice as a method to reduce turtle bycatch. The use of Zote™ soap has shown considerable promise to reduce bycatch of aquatic turtles when used with trotlines but its effectiveness in hoop nets has not been evaluated. We sought to determine the effectiveness of hoop nets baited with cheese bait or Zote™ soap and trotlines baited with shad or Zote™ soap as a way to sample channel catfish and prevent capture of aquatic turtles. We used a repeated-measures experimental design and treatment combinations were randomly assigned using a Latin-square arrangement. Eight sampling locations were systematically selected and then sampled with either hoop nets or trotlines using Zote™ soap (both gears), waste cheese (hoop nets), or cut shad (trotlines). Catch rates did not statistically differ among the gear–bait-type combinations. Size bias was evident with trotlines consistently capturing larger sized channel catfish compared to hoop nets. Results from a Monte Carlo bootstrapping procedure estimated the number of samples needed to reach predetermined levels of sampling precision to be lowest for trotlines baited with soap. Moreover, trotlines baited with soap caught no aquatic turtles, while hoop nets captured many turtles and had high mortality rates. We suggest that Zote™ soap used in combination with multiple hook sizes on trotlines may be a viable alternative to sample channel catfish and reduce bycatch of aquatic turtles.

  16. Invasion of South American suckermouth armoured catfishes Pterygoplichthys spp. (Loricariidae) in Kerala, India - a case study

    OpenAIRE

    A. Bijukumar; R. Smrithy; U. Sureshkumar; S. George

    2015-01-01

    This paper documents the occurrence of the exotic South American suckermouth armoured catfishes (Loricariidae) of the genus Pterygoplichthys spp. in the drainages of Thiruvananthapuram City, Kerala.  The morphological taxonomy revealed that the specimens are closely related to Pterygoplichthys disjunctivus (Weber, 1991) and P. pardalis (Castelnau, 1855), in addition to intermediary forms of unknown identity.  DNA barcoding using the mitochondrial DNA cytochrome c oxidase 1 (CO1) also failed t...

  17. Responses of Antioxidant Enzymes in Catfish Exposed to Liquid Crystals from E-Waste

    Directory of Open Access Journals (Sweden)

    Hongtao Yu

    2008-06-01

    Full Text Available Liquid crystals (LCs are typically elongated organic molecules with a non-uniform distribution of electrical charges leading to a dipole. LCs are widely used in displays of computers and other electronic devices. The rapid obsolescence rate of electronics results in large amounts of liquid crystal displays (LCDs entering the environment. Data on health effects of LCs on living creatures are currently limited to some acute toxicity tests by a few major LC manufacturers. These tests concluded that the vast majority of LCs are not acutely toxic. Since the amount of LCs in electronic devices is very small, the health effects of LCs at low concentrations or doses become important. Catfish were used as the test animals in this study. Four major enzymes of the fish’s antioxidant defense system catalase (CAT, superoxide dismutase (SOD, selenium-dependent glutathione peroxidase (Se-GPx, and glutathione-Stransferase (GST were chosen as biomarkers to examine effects of LCs, which were taken from obsolete laptop personal computers made in the early 1990s. The catfish were fed with food containing different contents of LCs for 40 days. Activities of the four chosen enzymes in fish livers were assayed. The results showed that there were significant inductions of CAT, SOD, and Se-GPx activities in response to the LC doses. The plots of the enzyme activities versus LC doses suggested an occurrence of oxidative stress when the dose reached about 20 μg LC/g fish·d. It was concluded that LCs can cause pollutant-induced stress to catfish at low doses. CAT, SOD and Se-GPx are effective biomarkers to give early warning on potential health effects of LCs on some aquatic lives including catfish.

  18. Histopathology of the gill and liver tissues of the African catfish ...

    African Journals Online (AJOL)

    An investigation on the effect of the heavy metal, lead (Pb) on the gill and liver of the African catfish Clarias gariepinus was carried out in the laboratory. One hundred and sixty (160) fingerlings of the fish were exposed to continuous exposure to sub-lethal concentrations (0.006 mg/l and 0.008 mg/l) of lead for a period of ...

  19. Detection of Off-Flavor in Catfish Using a Conducting Polymer Electronic-Nose Technology

    Directory of Open Access Journals (Sweden)

    Alphus D. Wilson

    2013-11-01

    Full Text Available The Aromascan A32S conducting polymer electronic nose was evaluated for the capability of detecting the presence of off-flavor malodorous compounds in catfish meat fillets to assess meat quality for potential merchantability. Sensor array outputs indicated that the aroma profiles of good-flavor (on-flavor and off-flavor fillets were strongly different as confirmed by a Principal Component Analysis (PCA and a Quality Factor value (QF > 7.9 indicating a significant difference at (P < 0.05. The A32S e-nose effectively discriminated between good-flavor and off-flavor catfish at high levels of accuracy (>90% and with relatively low rates (≤5% of unknown or indecisive determinations in three trials. This A32S e-nose instrument also was capable of detecting the incidence of mild off-flavor in fillets at levels lower than the threshold of human olfactory detection. Potential applications of e-nose technologies for pre- and post-harvest management of production and meat-quality downgrade problems associated with catfish off-flavor are discussed.

  20. Survival strategies of sharptooth catfish Clarias gariepinus in desiccating pans in the northern Kruger National Park

    Directory of Open Access Journals (Sweden)

    B.C.W. Van der Waal

    1998-08-01

    Full Text Available Observations in drying out pans showed that small (26-37 cm sharptooth catfish (Clarias gariepinus can hide at the bottom of small pools filled with sticky mud whereas larger fish stay afloat at the surface in larger pools with sloppy mud, where they easily become prey or succumb to heat stress. The inability of larger fish to keep down in the sloppy mud of up to 40 cm depth is the result of their large bulk and high density of the mud. This may indicate a survival advantage for smaller fish in the final dry-out phase of pools and is supported by the presence of only small fish remains in the last drying up pools of dry pans. Another adaptation of smaller fish includes the temporary congregation outside the water enabling concealment under dense vegetation as a means to escape adverse environmental conditions, including high water temperatures and avian predation. The advantage small fish have over larger catfish under these extreme conditions may explain why catfish are known to show a wide variation in growth rate under natural and aquaculture conditions.

  1. Inside the guts of wood-eating catfishes: can they digest wood?

    Science.gov (United States)

    German, Donovan P

    2009-11-01

    To better understand the structure and function of the gastrointestinal (GI) tracts of wood-eating catfishes, the gross morphology, length, and microvilli surface area (MVSA) of the intestines of wild-caught Panaque nocturnus, P. cf. nigrolineatus "Marañon", and Hypostomus pyrineusi were measured, and contrasted against these same metrics of a closely related detritivore, Pterygoplichthys disjunctivus. All four species had anatomically unspecialized intestines with no kinks, valves, or ceca of any kind. The wood-eating catfishes had body size-corrected intestinal lengths that were 35% shorter than the detritivore. The MVSA of all four species decreased distally in the intestine, indicating that nutrient absorption preferentially takes place in the proximal and mid-intestine, consistent with digestive enzyme activity and luminal carbohydrate profiles for these same species. Wild-caught Pt. disjunctivus, and P. nigrolineatus obtained via the aquarium trade, poorly digested wood cellulose (<33% digestibility) in laboratory feeding trials, lost weight when consuming wood, and passed stained wood through their digestive tracts in less than 4 h. Furthermore, no selective retention of small particles was observed in either species in any region of the gut. Collectively, these results corroborate digestive enzyme activity profiles and gastrointestinal fermentation levels in the fishes' GI tracts, suggesting that the wood-eating catfishes are not true xylivores such as beavers and termites, but rather, are detritivores like so many other fishes from the family Loricariidae.

  2. Evaluation of sodium carbonate peroxyhydrate as a potential catfish egg disinfectant.

    Science.gov (United States)

    Small, Brian C

    2009-06-01

    Two experiments were conducted to evaluate the efficacy of sodium carbonate peroxyhydrate (SCP) in improving the hatching success of channel catfish Ictalurus punctatus when used as a prophylactic chemotherapeutant during egg incubation. In the first experiment, the efficacy of SCP was evaluated in 379-L aluminum incubation troughs similar to those used in commercial hatcheries. Egg masses treated daily with 254 mg of SCP/L of water had significantly higher mean hatching success than untreated controls, and a pathogen-inhibiting effect was also evident (i.e., no gross infection was observed on the treated egg masses). In the second experiment, the hatching success of egg masses treated daily with 254 mg/L was compared with that of egg masses treated daily with hydrogen peroxide (70 mg/L). The effects of both treatments on the pH, dissolved oxygen, and hydrogen peroxide concentrations in the trough were also examined. Both SCP and hydrogen peroxide significantly improved hatching success. Unlike in the treatment with hydrogen peroxide, water pH increased during the treatment with SCP; however, no negative effects on hatching success were observed. The results of this research suggest that SCP acts similarly to hydrogen peroxide in improving channel catfish hatching success and warrants further research to determine whether it could be a practical and effective alternative for managing catfish egg infections in commercial hatcheries.

  3. Impact of a widely cultivated tree (moringa oleifera) on the health of commercially important hybrid catfish

    International Nuclear Information System (INIS)

    Adamu, K.M.; Ahmed, I.M.

    2014-01-01

    Plantations of the tree Moringa oleifera often lead to increase levels of leaf dust in nearby freshwater environments, and there is concern that, this could have a negative impact on catfish, which are important for aquaculture. Therefore, this study, determined the biochemical alterations in serum, liver and kidney of hybrid catfish (Clarias gariepinus ( ) Heterobranchus bidorsalis ( ) exposed to sub-lethal concentrations of M. oleifera leaf dust in a static renewable bioassay system during a medium term exposure period. The fish (mean length, 16.33 cm, mean weight, 9.90 g) were exposed to 0.16, 0.12, 0.08, 0.04 and 0.00 mg/L concentrations of the plant leaf dust in triplicate exposure. After 21-days of exposure period, the fish were sacrificed for the biochemical parameters: glucose, total protein, albumin, globulin, cholesterol, triglyceride, g-glutamyltransferase, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase. Fish exposed to leaf dust showed significant differences (P 0.05), in the liver and kidney, respectively, in the test fish. Other parameters were not significantly different (P>0.05) in their respective tissue-organs. Ipso-facto, the alteration in biochemical parameters of hybrid catfish exposed to M. oleifera leaf dust was concentration dependent with 0.16 mg/L showing the highest negative alterations thus fish exposed to concentrations above 0.16 mg/L for longer durations may suffer impaired health effect. (author)

  4. Antibiotic Application and Emergence of Multiple Antibiotic Resistance (MAR) in Global Catfish Aquaculture.

    Science.gov (United States)

    Chuah, Li-Oon; Effarizah, M E; Goni, Abatcha Mustapha; Rusul, Gulam

    2016-06-01

    Catfish is one of the most cultivated species worldwide. Antibiotics are usually used in catfish farming as therapeutic and prophylactic agents. In the USA, only oxytetracycline, a combination of sulfadimethoxine and ormetoprim, and florfenicol are approved by the Food Drug Administration for specific fish species (e.g., catfish and salmonids) and their specific diseases. Misuse of antibiotics as prophylactic agents in disease prevention, however, is common and contributes in the development of antibiotic resistance. Various studies had reported on antibiotic residues and/or resistance in farmed species, feral fish, water column, sediments, and, in a lesser content, among farm workers. Ninety percent of the world aquaculture production is carried out in developing countries, which lack regulations and enforcement on the use of antibiotics. Hence, efforts are needed to promote the development and enforcement of such a regulatory structure. Alternatives to antibiotics such as antibacterial vaccines, bacteriophages and their lysins, and probiotics have been applied to curtail the increasing emergence of antibiotic-resistant bacteria due to the imprudent application of antibiotics in aquaculture.

  5. Comparing the efficacy of four immersion flush and static hydorgen peroxide and copper sulfate treatments on channel catfish eggs infected with water molds

    Science.gov (United States)

    Water mold infestations on channel catfish eggs lower the hatch rate (egg survival) and ultimately the number of catfish fry available for stocking in production ponds. This study compared the potential of two hydrogen peroxide (HP) and two copper sulfate pentahydrate (CSP) treatments to increase c...

  6. Pond-raised hybrid catfish, male Ictalurus punctatus X female Ictalurus furcatus, do not respond to microbial phytase “super-dosing”

    Science.gov (United States)

    Two experiments were conducted in consecutive years to evaluate responses of hybrid catfish, male Ictalurus punctatus X female Ictalurus furcatus, to “super-dosing” of 6-phytase added to existing commercial catfish feeds. In each experiment, two diets with or without a phytase super-dose (2,500 and ...

  7. Example of the application the microsatellite DNA fragments in the study of farmed European catfish (Silurus glanis, L. broodstock

    Directory of Open Access Journals (Sweden)

    Kuciñski Marcin

    2015-06-01

    Full Text Available European catfish, Silurus glanis L., is the second largest freshwater fish in Europe. The species is very popular among the farmers, as it is one of the most promising European aquaculture species. Despite the growing importance of European catfish in freshwater aquaculture, the genetic data available on this species are still limited. The main purpose of the present study was to develop a reliable, feasible genetic protocol for future studies on European catfish populations and broodstocks in Poland. The genetic characteristics of the tested fish group were based on genetic parameters such as the polymorphism information content (PIC, the effective population size (Ne, the inbreeding coefficient (Fis, and the Garza-Williamson index (M, among others. Additionally, the potential effects of a genetic bottleneck on the genetic variation of the broodstock were examined. The genetic analysis protocol described in this study can be used to establish genetic-based records for European catfish broodstocks, including for sperm cryobanking. This approach will be useful for elaborating the selection procedures that allow for optimal assemblages of spawning pairs in artificial reproduction. The application of the genetic analysis protocol in practice will permit maintaining high quality in European catfish broodstocks.

  8. Frequency and gravity of human envenomations caused by marine catfish (suborder siluroidei): a clinical and epidemiological study.

    Science.gov (United States)

    Haddad, Vidal; Martins, Itamar Alves

    2006-06-15

    Catfish occur in marine and freshwater environments worldwide. They have three serrated venomous bony stings in the dorsal and pectoral fins that are used for defence against predators and are refilled by glandular tissues under the epithelium. However, some catfishes do not have poisonous glands next to the sting and cause traumatic wounds without poisoning. The objective of this study was to provide data for, and comment on, the epidemiological and clinical problems caused by marine catfish. The authors have observed, followed and documented 127 injuries caused by marine catfish stings during different phases of the envenoming over a time period of 8 years at three points along the Western Atlantic Ocean coast. The patients presented intense pain during the acute phase of envenoming and complications, such as bacterial and fungi infections and retention of bony fragments, in the later phase. Immersion of the affected extremity in hot water was used in about 20% of cases with excellent results. Injuries caused by marine catfish are common (about 20% of injuries caused by marine animals in a series of more than 700 injuries recorded by the author) and cause intense pain and later complications. Immersion of the affected extremity in hot water results in improvement in the acute phase, but does not prevent the appearance of secondary infection or foreign body reactions.

  9. Determination and health risk assessment of enrofloxacin, flumequine and sulfamethoxazole in imported Pangasius catfish products in Thailand.

    Science.gov (United States)

    Jansomboon, Worawat; Boontanon, Suwanna Kitpati; Boontanon, Narin; Polprasert, Chongrak

    2018-02-01

    The goals of this study were to determine the levels of three antibiotics - enrofloxacin, flumequine and sulfamethoxazole - in Pangasius catfish products imported into Thailand and to assess the health risks from consumption. To extract these antibiotic residues, acetonitrile, methanol and a small amount of formic acid were used as solvents. Determination of the antibiotics after extraction steps was carried out by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) technique. The results showed that 14 and 3 samples of Pangasius catfish products were contaminated with enrofloxacin and sulfamethoxazole, respectively. No flumequine residue was found. While the concentration levels of these antibiotics in most contaminated samples were lower than the European Union (EU) standard, one sample was found to contain sulfamethoxazole at 245.91 µg kg -1 , which was higher than the EU standard (100 µg kg -1 ), indicating the likelihood that some contaminated freshwater fish products are widely distributed in Thai markets. Notably, the concentration levels of enrofloxacin in samples of Pangasius catfish with skin were higher than in non-skin products, suggesting that products with skin might retain more antibiotic residues than non-skin products. Although the hazard quotient showed that consuming imported Pangasius catfish products, based on the current consumption rate, will not adversely affect consumer health, antibiotic residues in Pangasius catfish products imported into Thailand should be continually monitored.

  10. Potential interactions between metazoan parasites of the Mayan catfish Ariopsis assimilis and chemical pollution in Chetumal Bay, Mexico.

    Science.gov (United States)

    Vidal-Martínez, V M; Aguirre-Macedo, M L; Noreña-Barroso, E; Gold-Bouchot, G; Caballero-Pinzón, P I

    2003-06-01

    The effect of pollutants on the intensity of infection of metazoan parasites in the Mayan catfish, Ariopsis assimilis was investigated. Data were collected on pollutants and metazoan parasites from 76 catfish from five localities in Chetumal Bay in October, 1996. Nineteen pollutants (pesticides, polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs)) were found in the catfish livers. Heavy metal content was not determined. Nineteen metazoan parasite species were recovered. After controlling for fish length and sampling station, there was a significant negative linear relationship between the intensity of the larval digenean Mesostephanus appendiculatoides and 1,1,1,-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) concentrations. This negative relationship may be explained either by the effect of the pesticide on the mortality of (i) free-living larval forms, (ii) metacercariae in the fish, (iii) infected fish or (iv) intermediate host snails. There were significant differences between fish parasitized and not parasitized with M. appendiculatoides with respect to their DDT concentrations. There were also significant differences between the variances of the mean Clark's coefficient of condition values between catfish parasitized and not parasitized by M. appendiculatoides, with the variance of non-parasitized catfish being significantly larger. The results provided statistical evidence that DDT has a detrimental effect on M. appendiculatoides infection intensity. Furthermore, the significantly larger variance value of Clark's coefficient for non-parasitized fish suggested that DDT affects both the parasite and general host condition.

  11. A comparison of dioxins, dibenzofurans and coplanar PCBs in uncooked and broiled ground beef, catfish and bacon.

    Science.gov (United States)

    Schecter, A; Dellarco, M; Päpke, O; Olson, J

    1998-01-01

    The primary source of dioxins (PCDDs), dibenzofurans (PCDFs) and coplanar PCBs for the general population is food, especially meat, fish, and dairy products. However, most data on the levels of these chemicals is from food in the raw or uncooked state. We report here the effect of one type of cooking (broiling) on the levels of PCDDs, PCDFs, and coplanar PCBs in ground beef (hamburger), bacon and catfish. Samples of hamburger, bacon, and catfish were broiled and compared to uncooked samples in order to measure changes in the amounts of dioxins in cooked food. The total amount of PCDD, PCDF, and coplanar PCB TEQ decreased by approximately 50% on average for each portion as a result of broiling the hamburger, bacon and catfish specimens. The mean concentration (pg TEQ/kg, wet weight) of PCDDs, PCDFs, and coplanar PCBs, however, remained the same in the hamburger, increased by 83% in the bacon, and decreased by 34% in the catfish. On average, the total measured concentration (pg/kg) of the congeners of PCDDs, PCDFs, and coplanar PCBs increased 14% in the hamburger, increased 29% in the bacon, and decreased 33% in the catfish.

  12. Evaluation of the flathead catfish population and fishery on Lake Carl Blackwell, Oklahoma, with emphasis on the effects of noodling

    Science.gov (United States)

    Winkelman, Dana L.; Michaletz, Paul H.; Travnichek, Vincent H.

    2011-01-01

    I conducted a 3-year study at Lake Carl Blackwell, Oklahoma to estimate effects of various fishing gears on the flathead catfish Pylodictis olivaris population. Managers were particularly interested in the effect of handfishing or noodling on this population. I used a phone survey to assess angler effort and electrofishing and gill nets to calculate standard population metrics to assess composition of the current population. Survey data indicated that fishing effort and harvest were highest for trotlines and juglines and lowest for noodling. Size distribution of fish harvested by noodlers was not different from sizes that were available in the fishery and was similar to those fish harvested with other gears. Flathead catfish Sampled in Lake Carl Blackwell ranged in size from 38 to 1,220 mm total length, and 77% of the population was less than 508 mm (minimum legal size). Estimated total annual mortality was about 11%. Proportional size distribution (PSD) of flathead catfish for Lake Carl Blackwell indicates that about 70% of legal-sized flathead catfish were over the preferred size of 710 mm. Overall, the Lake Carl Blackwell flathead catfish population appeared healthy. There were a wide range of sizes and ages in the population, and PSD indicated a well-balanced population with many preferred and memorable-sized fish. Due to its rarity, noodling is probably not adversely influencing the population. Additionally, noodling at Lake Carl Blackwell does not appear to be as size-selective as previously thought. 

  13. Exploration of indigenous bacteria in an intensive aquaculture system of African catfish (Clarias sp.) in Banyuwangi, Indonesia

    Science.gov (United States)

    Prayogo; Rahardja, B. S.; Asshanti, A. N.; Dewi, N. N.; Santanumurti, M. B.

    2018-04-01

    Intensive African catfish culture in tarpaulin pond was popular in Banyuwangi, Indonesia since the government supported the fisheries sector. Unfortunately, the failure of African catfish culture still occurred since the waste from fish metabolite process and feed residue decreased the water quality. Bacteria in the water could be the solution to increase the success rate of aquaculture by improving the water quality. This study purpose was to obtained indigenous bacteria in intensive aquaculture system of African catfish to improve water quality. This study successfully isolated bacteria contained with amylase, protease and lipase characteristic. Isolated bacteria in this study were identified as Pseudomonas pseudomallei (97.81%), Bacillus subtilis (95.81%) and Pseudomonas stutzeri (61.21%).

  14. The complete mitochondrial genome of the threatened Neotropical catfish Lophiosilurus alexandri (Silurifomes: Pseudopimelodidae and phylogenomic analysis indicate monophyly of Pimelodoidea

    Directory of Open Access Journals (Sweden)

    Daniel Cardoso Carvalho

    Full Text Available Abstract Lophiosilurus alexandri is an endemic catfish from the São Francisco River Basin (Brazil popularly known as pacamã, which has economic potential for aquaculture farming. The mitochondrial genome was sequenced for the threatened Neotropical catfish L. alexandri. Assembly into scaffolds using MIRA and MITObim software produced the whole, circularized mitochondrial genome, which comprises 16,445 bp and presents the typical gene arrangement of Teleostei mitochondria. A phylogenomic analysis was performed after the concatenation of all proteins obtained from whole mitogenomes of 20 Siluriformes and two outgroups. The results confirmed the monophyly of nine families of catfishes and also clustered L. alexandri as a sister group to the family Pimelodidae, thus confirming the monophyly of the superfamily Pimelodoidea. This is the first mitochondrial phylogenomics study for Pimelodoidea and the first mitogenome described for the Pseudopimelodidae family, representing an important resource for phylogeography, evolutionary biology, and conservation genetics studies in Neotropical fishes.

  15. Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco) and Its Active Site for Chemotaxis

    Science.gov (United States)

    Dawar, Farman Ullah; Tu, Jiagang; Xiong, Yang; Lan, Jiangfeng; Dong, Xing Xing; Liu, Xiaoling; Khattak, Muhammad Nasir Khan; Mei, Jie; Lin, Li

    2016-01-01

    Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA), a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase) activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we have discovered several novel immune-relevant proteins in yellow catfish skin mucus by mass spectrometry (MS). The CypA present among them was further detected by Western blot. Moreover, the CypA present in the skin mucus displayed strong chemotactic activity for yellow catfish leucocytes. Interestingly, asparagine (like arginine in mammals) at position 69 was the critical site in yellow catfish CypA involved in leucocyte attraction. These novel efforts do not only highlight the enzymatic texture of skin mucus, but signify CypA to be targeted for anti-inflammatory therapeutics. PMID:27589721

  16. Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco and Its Active Site for Chemotaxis

    Directory of Open Access Journals (Sweden)

    Farman Ullah Dawar

    2016-08-01

    Full Text Available Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA, a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we have discovered several novel immune-relevant proteins in yellow catfish skin mucus by mass spectrometry (MS. The CypA present among them was further detected by Western blot. Moreover, the CypA present in the skin mucus displayed strong chemotactic activity for yellow catfish leucocytes. Interestingly, asparagine (like arginine in mammals at position 69 was the critical site in yellow catfish CypA involved in leucocyte attraction. These novel efforts do not only highlight the enzymatic texture of skin mucus, but signify CypA to be targeted for anti-inflammatory therapeutics.

  17. A model to environmental monitoring based on glutathione-S-transferase activity and branchial lesions in catfish

    Science.gov (United States)

    Neta, Raimunda Nonata Fortes Carvalho; Torres, Audalio Rebelo

    2017-11-01

    In this work, we validate the glutathione-S-transferase and branchial lesions as biomarkers in catfish Sciades herzbergii to obtain a predictive model of the environmental impact effects in a harbor of Brazil. The catfish were sampled from a port known to be contaminated with heavy metals and organic compounds and from a natural reserve in São Marcos Bay, Maranhão. Two biomarkers, hepatic glutathione S-transferase (GST) activity and branchial lesions were analyzed. The values for GST activity were modeled with the occurrence of branchial lesions by fitting a third order polynomial. Results from the mathematical model indicate that GST activity has a strong polynomial relationship with the occurrence of branchial lesions in both the wet and the dry seasons, but only at the polluted port site. Our mathematic model indicates that when the GST ceases to act, serious branchial lesions are observed in the catfish of the contaminated port area.

  18. Length-weight relationship of eleven species of marine catfishes from the northern Arabian Sea coast of Pakistan

    Science.gov (United States)

    Farooq, Noureen; Qamar, Nazia; Rashid, Shahnaz; Panhwar, Sher Khan

    2017-09-01

    This study records length-weight relationships (LWRs) for eleven commercially important marine catfish species of the family Ariidae (sea catfishes) and Plotosidae (eel catfishes) from the northern Arabian Sea coast of Pakistan. The specimens were sampled from December 2014 to November 2015, using bottom trawls with various mesh sizes by commercial vessels. The species were Nemapteryx caelatus, Sciades sona, Arius gagora, Batrachocephalus mino, Netuma thalassina, N. bilineata, Osteogeneiosus militaris, Plicofollis dussumieri, P. tenuispinis, Plotosus limbatus, and P. lineatus. Of the eleven species, two species A. gagora and N. bilineata were recorded for the first time and LWRs for four species A. gagora, N. bilineata, S. sona, and B. mino still have no data in the FishBase database. In addition, new maximum length for each of the three species N. bilineata, O. militaris and B. mino was also found.

  19. Interactions between non-native armored suckermouth catfish (Loricariidae: Pterygoplichthys) and native Florida manatee (Trichechus manatus latirostris) in artesian springs

    Science.gov (United States)

    Nico, Leo G.; Loftus, William F.; Reid, James P.

    2009-01-01

    Non-native suckermouth armored catfishes (Loricariidae) of the genus Pterygoplichthys are now common throughout much of peninsular Florida. In this paper, we present preliminary observations on interactions between a Pterygoplichthys species, tentatively identified as P. disjunctivus (Weber, 1991), and endangered native Florida manatees, Trichechus manatus latirostris (Harlan, 1824), in artesian spring systems in Florida's St. Johns River drainage. The introduced catfish have become abundant in spring habitats, sites used by manatees as winter thermal refuges. In the spring runs, Pterygoplichthys regularly attaches to manatees and grazes the epibiota on their skin. On occasion, dozens of Pterygoplichthys congregate on individual manatees. Manatee responses varied widely; some did not react visibly to attached catfish whereas others appeared agitated and attempted to dislodge the fish. The costs and/or benefits of this interaction to manatees remain unclear.

  20. Spatial and seasonal variations on Henneguya exilis prevalence on cage intensive cultured channel catfish (Ictalurus punctatus, in Tamaulipas, Mexico

    Directory of Open Access Journals (Sweden)

    Jaime Luis Rábago-Castro

    2013-03-01

    Full Text Available Diseases are of particular importance for aquaculture worldwide, particularly in intensive culture. In Mexico, intensive culture of channel catfish is mainly done in floating cages. The aim of the present study was to determine the presence of the myxozoan Henneguya and the effect of site, period and host length on its prevalence in cage-cultured channel catfish. Over a year, fish were examined on six different sites. Results showed the presence of Henneguya exilis in all the farms. However, no significant effects were observed for site and season on prevalence, nor was there a correlation between host length and infection prevalence.

  1. Prevalence of pansteatitis in African sharptooth catfish, Clarias gariepinus (Burchell, in the Kruger National Park, South Africa

    Directory of Open Access Journals (Sweden)

    K. David A. Huchzermeyer

    2012-04-01

    Full Text Available Pansteatitis was confirmed in sharptooth catfish, Clarias gariepinus (Burchell, from three main locations within the Kruger National Park (KNP; the Olifants River Gorge, Engelhard Dam on the Letaba River and from the Sabie River in the Sabiepoort. An increasing prevalence of pansteatitis was observed in catfish during repeated samplings from the Olifants Gorge from 2009 to 2011 and co-existence of old and recent lesions indicated on-going incitement of pansteatitis. Only a low prevalence of pansteatitis was observed in catfish sampled from the Olifants River upstream of the Gorge in the KNP and no pansteatitis was observed in catfish sampled from a rain-filled dam not connected to the Olifants River. Common to both the Olifants Gorge and the Sabiepoort is the damming of the rivers in Mozambique to form lakes Massingir and Corumana respectively. Anthropogenic activities resulting in potential pollution of the rivers differ greatly between these two catchments, providing argument against a primary pollution-related aetiology of the pansteatitis found at these two sites. Compared with other sites, analysis of stomach contents of catfish from the Olifants Gorge and the Sabiepoort strongly suggested that consumption of a predominantly fish diet was associated with the development of pansteatitis in these fish. In a farmed population of catfish used as positive control, development of pansteatitis could be ascribed to consumption of rancid fish waste from a trout slaughterhouse. In the Olifants Gorge, alien invasive silver carp, Hypophthalmychthys molitrix (Valenciennes, seasonally migrate upstream out of Lake Massingir to spawn. This schooling species is an obligate phytoplankton feeder with consequent high levels of adipose tissue n-3 polyunsaturated fatty acids. In the Olifants Gorge, at least, this may explain seasonal exposure to levels of polyunsaturated fats in the diets of catfish and crocodiles to which these animals are not adapted. The

  2. Prevalence of pansteatitis in African sharptooth catfish, Clarias gariepinus (Burchell, in the Kruger National Park, South Africa

    Directory of Open Access Journals (Sweden)

    K. David A. Huchzermeyer

    2012-11-01

    Full Text Available Pansteatitis was confirmed in sharptooth catfish, Clarias gariepinus (Burchell, from three main locations within the Kruger National Park (KNP; the Olifants River Gorge, Engelhard Dam on the Letaba River and from the Sabie River in the Sabiepoort. An increasing prevalence of pansteatitis was observed in catfish during repeated samplings from the Olifants Gorge from 2009 to 2011 and co-existence of old and recent lesions indicated on-going incitement of pansteatitis. Only a low prevalence of pansteatitis was observed in catfish sampled from the Olifants River upstream of the Gorge in the KNP and no pansteatitis was observed in catfish sampled from a rain-filled dam not connected to the Olifants River. Common to both the Olifants Gorge and the Sabiepoort is the damming of the rivers in Mozambique to form lakes Massingir and Corumana respectively. Anthropogenic activities resulting in potential pollution of the rivers differ greatly between these two catchments, providing argument against a primary pollution-related aetiology of the pansteatitis found at these two sites. Compared with other sites, analysis of stomach contents of catfish from the Olifants Gorge and the Sabiepoort strongly suggested that consumption of a predominantly fish diet was associated with the development of pansteatitis in these fish. In a farmed population of catfish used as positive control, development of pansteatitis could be ascribed to consumption of rancid fish waste from a trout slaughterhouse. In the Olifants Gorge, alien invasive silver carp, Hypophthalmychthys molitrix (Valenciennes, seasonally migrate upstream out of Lake Massingir to spawn. This schooling species is an obligate phytoplankton feeder with consequent high levels of adipose tissue n-3 polyunsaturated fatty acids. In the Olifants Gorge, at least, this may explain seasonal exposure to levels of polyunsaturated fats in the diets of catfish and crocodiles to which these animals are not adapted. The

  3. Prevalence of pansteatitis in African sharptooth catfish, Clarias gariepinus (Burchell), in the Kruger National Park, South Africa.

    Science.gov (United States)

    Huchzermeyer, K David A

    2012-11-09

    Pansteatitis was confirmed in sharptooth catfish, Clarias gariepinus (Burchell), from three main locations within the Kruger National Park (KNP); the Olifants River Gorge, Engelhard Dam on the Letaba River and from the Sabie River in the Sabiepoort. An increasing prevalence of pansteatitis was observed in catfish during repeated samplings from the Olifants Gorge from 2009 to 2011 and co-existence of old and recent lesions indicated on-going incitement of pansteatitis. Only a low prevalence of pansteatitis was observed in catfish sampled from the Olifants River upstream of the Gorge in the KNP and no pansteatitis was observed in catfish sampled from a rain-filled dam not connected to the Olifants River. Common to both the Olifants Gorge and the Sabiepoort is the damming of the rivers in Mozambique to form lakes Massingir and Corumana respectively. Anthropogenic activities resulting in potential pollution of the rivers differ greatly between these two catchments, providing argument against a primary pollution-related aetiology of the pansteatitis found at these two sites. Compared with other sites, analysis of stomach contents of catfish from the Olifants Gorge and the Sabiepoort strongly suggested that consumption of a predominantly fish diet was associated with the development of pansteatitis in these fish. In a farmed population of catfish used as positive control, development of pansteatitis could be ascribed to consumption of rancid fish waste from a trout slaughterhouse. In the Olifants Gorge, alien invasive silver carp, Hypophthalmychthys molitrix (Valenciennes), seasonally migrate upstream out of Lake Massingir to spawn. This schooling species is an obligate phytoplankton feeder with consequent high levels of adipose tissue n-3 polyunsaturated fatty acids. In the Olifants Gorge, at least, this may explain seasonal exposure to levels of polyunsaturated fats in the diets of catfish and crocodiles to which these animals are not adapted. The possible roles of

  4. The influence of supplemented Curcuma in feed formulation to improve growth rate and feed efficiency of catfish (Clarias sp.)

    Science.gov (United States)

    Ulum, M. M.; Zubaidah, M.; Arief, M.; Prayogo

    2018-04-01

    Catfish (Clarias sp.) is very potential to be developed as a food fish. The use of the feed additive in feed intended to improve the health, productivity and compliance with animal nutrition. Feed additive is to use ginger are derived from nature.The purpose of this study was to determine the potential increase in feed formulation. The Curcuma effect on growth rate and feed efficiency of catfish (Clarias sp.). The method used experimental methods and design complete random with 4 treatments and 5 replications. The parameters examined in this study aregrowth rate and efficiency feed.The research showed that the ANOVA markedly dissimilar ( Padditive on formulations feed.

  5. Impacts of Catfish Effluents on Water Quality Parameters of Majidun Stream, South-West, Nigeria

    Directory of Open Access Journals (Sweden)

    O. E. Omofunmi

    2017-06-01

    Full Text Available There has been a great concern about the level of safety of surface waters, especially in developing countries where there is an exponential increase in water pollution and water-borne diseases. The aim of this study was to assess the effect of catfish pond effluents on water quality of stream water where five catfish farms were located. Water samples were taken on monthly basis, 20 cm of below water surface from the streams that receive effluents from neighboring fishponds. Water quality indicators like dissolved oxygen, biochemical oxygen demand (BOD5, nitrate, nitrite, water temperature, ammonia and Hydrogen ion Concentration (pH were examined in the sampled waters in accordance with the American Public Health Association standards. The average values of water quality indicators examined at effluents and non-effluents discharged sites of the stream indicated that water (24.6 ± 0.2, 24.2 ±0.1, (7.29±0.30, 7.30±0.10, (6.90±0.4, 7.07±0.1 mg/l, (0.40±0.04, 0.27±0.01, (3.77±0.26, 2.34±0.16 mg/l, (3.59±0.11, 2.80±0.02 mg/l and (3.51±0.24, 2.46±0.21 mg/l at (p≥0.05 respectively for temperature, pH, dissolved oxygen, total ammonia, total nitrogen, total phosphorus, and BODs. They were significant differences (P 0.05 excepts temperature and pH, between values obtained at effluents discharged and non-effluents discharged sites, indicating that improper discharges of catfish pond effluents could resulted into environmental contamination

  6. THE ABILITY OF FAST-GROWING TRANSGENIC AFRICAN CATFISH (Clarias gariepinus ON PREDATOR AVOIDANCE

    Directory of Open Access Journals (Sweden)

    Huria Marnis

    2016-12-01

    Full Text Available Research Institute for Fish Breeding has produced transgenic African catfish (Clarias gariepinus containing stripped catfish growth hormone gene (PccBA-PhGH with growth 19.86% faster than that of non-transgenic fish. This fish has high potential to be released and utilized for fish farming sector to increase national production. However, there is not yet information about environmental risk of this fish. One of the major fitness traits determining potential environmental risk is predator avoidance. This study aimed to determine the predator avoidance ability of transgenic African catfish in an experimental laboratory condition. In this study, thirty five individuals each of transgenic and non-transgenic with body weight of about 0.1 ± 0.019 g were communally stocked in 60 cm x 40 cm x 40 cm aquarium with limited feeding frequency (ad libitum twice a day. One day after the fish were stocked, the predators were added to each aquarium. The non-transgenic and transgenic with body weight of 1.0 ± 0.024 g were stocked as predators as many as five individual in each aquarium. After approximately two weeks of predation, all remaining fish were collected for transgenic verification by PCR method. Genomic DNA was isolated from fin tissue of individually survivors. The results of this study showed that the transgenic fish had worse predator avoidance and lower cannibal than non-transgenic (P0.05 in limited food. The transgenic fish may have lower fitness than non-transgenic.

  7. Bioaccumulation factor for 32P measured in bluegill, Lepomis macrochirus, and catfish, Ictalurus punctatus

    International Nuclear Information System (INIS)

    Kahn, B.; Turgeon, K.S.; Martini, D.K.; Dunkerly, S.J.; el-Shinawy, R.M.; Wilson, M.D.

    1987-01-01

    The ratio of the bioaccumulation factors for 32 P and phosphorus was determined for edible tissue in two species of freshwater fish by measuring the specific activity ( 32 P activity per milligram phosphorus) in muscle relative to feed. The 32 P tracer was added to the feed at a uniform level throughout the study. Feeding was at two levels: ad libitum and at a lower but constant intake per body weight. In the main experiment, bluegill were maintained in a large flow-through tank and sacrificed at approximately weekly intervals for 51 d of 32 P accumulation and 28 d of depuration to compare the specific activity with values predicted with a calculational model. In experiments performed in smaller aquaria, the specific activity in bluegill and catfish muscle was compared at two feeding levels and two temperatures. In addition, unfed fish were exposed to 32 P in water at a known specific activity to determine the extent of phosphorus uptake directly from water. The pattern of specific activity increase and decrease in fish muscle during the accumulation/depuration experiment was consistent with a one-compartment model, so that specific activity ratios at steady state could be predicted from measurements during relatively brief exposures. On this basis, the ratio of the bioaccumulation factors of 32 P and phosphorus in fish feeding ad libitum was 0.081 for bluegill and 0.17 for catfish. Hence, at a mean phosphorus bioaccumulation factor of 70,000, the factors for 32 P are 6000 and 12,000, respectively. The ratios were less at lower phosphorus intakes associated with lower feeding rates; moreover, the lesser value for bluegill occurred at a much lower phosphorus intake than by catfish

  8. Constraints to adoption of improved hatchery management practices among catfish farmers in Lagos State

    Directory of Open Access Journals (Sweden)

    Oghenetejiri DIGUN-AWETO

    2017-11-01

    Full Text Available Aquaculture has shown capacities to serve as means of livelihood, improve living standards, provide employment and generate foreign exchange in many countries. Recent investment in Nigerian aquaculture has been target towards catfish farming. However, small quantity and poor quality fish seeds are one of the problems limiting production. Consequently, Lagos State government introduced improved breeding and hatchery management practices as a package to fish hatchery operators with the aim of improving fish seed quantity and quality in the state. Nevertheless, the dissemination of the package has not yielded the desired result. This study assessed the constraints to adoption of improved hatchery management practices among catfish farmers in Lagos State. With structured questionnaire, 150 catfish farmers, randomly selected from 12 local government areas spread across Lagos State were interviewed. Despite that majority of the respondents strongly agreed or agreed to the fact that improved hatchery management practices have positive impacts on breeding, hatching, and survival of fish fry, majority of them affirmed that high cost of acquisition, high technicality in using the improved management practices as well as inadequate information about the improved management practices are primary reasons for non-adoption of some of the improved practices. Some of the limitations faced by the respondents include insufficient capital, lack of technical expertise to use the methods adequately, non-availability of inputs, expensive cost of facility maintenance, poor information dissemination and insufficient technical support from the extension agents and the state government. Although the adoption of improved practices has not been total, due to these constraints, the farmers’ knowledge of the improved hatchery management practices is broad. There is a need for the state government to subsidize the improved hatchery technologies and inputs, in addition to

  9. MOTIVATIONS FOR INVOLVEMENT IN URBAN CATFISH FARMING AND SUSTAINABLE FOOD SECURITY IN NIGERIA

    Directory of Open Access Journals (Sweden)

    Afolabi Monisola TUNDE

    2017-10-01

    Full Text Available Fish farming is known as one of the ways of ensuring food security and alleviating poverty through income generation. It is very common in the urban areas of Nigeria because of great demand for fish. This study determines motivations for involvement in urban catfish farming and sustainable food security in Ilorin, Kwara State, Nigeria. Specifically, the study identifies fish farmers and their socio-demographic characteristics in the study area; it examines motives of fish farming; examines the contributions of fish farming to household food security; and assesses the challenges faced by fish farmers for increased production. Both primary and secondary sources of information were employed and a total of 120 fish farmers were sampled randomly with copies of structured questionnaire. Frequency counts, percentages, tabulations and matrix ranking were employed to analyze the gathered data. The findings revealed that catfish production has actually contributed immensely to sustainable food security by making fish available, accessible and stable within the study area. The main motive for involvement in fish farming by the farmers includes food security (with a mean value of 3.80. This was followed by income supplement (x = 3.50, source of employment (x = 3.42 and increase in price of meat (x = 3.37. It can therefore be concluded that catfish farming in the study area has not only improve practitioners’ food security but has also contributed immensely to their income. The paper recommends the need to include fish farming in urban planning and that it should be encouraged in both rural and urban areas so as to have sustainable food security in the country as a whole.

  10. MICROSATELLITE GENETIC VARIATION IN CULTURED POPULATIONS OF AFRICAN CATFISH (Clarias gariepinus IN INDONESIA

    Directory of Open Access Journals (Sweden)

    Imron Imron

    2011-06-01

    Full Text Available African catfish, Clarias gariepinus, is one of economically important farmed species in Indonesia. To support the development of aquaculture industry, high genetic quality of both broodstock and seeds is required and breeding program is considered as viable option. Information on genetic variation of the populations being considered to form a base population may give insight toward the appropriate strategy to be implemented in breeding program. This study was aimed to assess genetic variation in farmed populations of catfish in Indonesia using microsatellite markers with special emphasis on their use to develop breeding program. Three populations of farmed catfish, namely Dumbo, Paiton, and Sangkuriang were collected. Fifteen individuals representing each population were screened for microsatellite variability using seven primer sets (cga01, cga02, cga03, cga05, cga06, cga09, cga10. Results found that with exception of two loci (cga01 and cg02 which had a slight increase, the other four loci showed reduction in the number of alleles ranging from 35% to 80% depending on loci. Farmed populations also showed heterozygote deficient and inbreeding level, being the highest was found in Sangkuriang and the least was observed in Dumbo population. Individuals within populations contributed most (95% while interpopulation variation accounted for only 5% of the total genetic variation. Populations of Dumbo and Sangkuriang were genetically similar while populations of Paiton were genetically different from both Dumbo and Sangkuriang. Viewed from genetic perspective, by combining all information emerging from this study, the best possible strategy to establish a base population with broad genetic base and less inbreeding would be to combine all the populations into a synthetic base population.

  11. Digestive enzyme activities and gastrointestinal fermentation in wood-eating catfishes.

    Science.gov (United States)

    German, Donovan P; Bittong, Rosalie A

    2009-11-01

    To determine what capabilities wood-eating and detritivorous catfishes have for the digestion of refractory polysaccharides with the aid of an endosymbiotic microbial community, the pH, redox potentials, concentrations of short-chain fatty acids (SCFAs), and the activity levels of 14 digestive enzymes were measured along the gastrointestinal (GI) tracts of three wood-eating taxa (Panaque cf. nigrolineatus "Marañon", Panaque nocturnus, and Hypostomus pyrineusi) and one detritivorous species (Pterygoplichthys disjunctivus) from the family Loricariidae. Negative redox potentials (-600 mV) were observed in the intestinal fluids of the fish, suggesting that fermentative digestion was possible. However, SCFA concentrations were low (<3 mM in any intestinal region), indicating that little GI fermentation occurs in the fishes' GI tracts. Cellulase and xylanase activities were low (<0.03 U g(-1)), and generally decreased distally in the intestine, whereas amylolytic and laminarinase activities were five and two orders of magnitude greater, respectively, than cellulase and xylanase activities, suggesting that the fish more readily digest soluble polysaccharides. Furthermore, the Michaelis-Menten constants (K(m)) of the fishes' beta-glucosidase and N-acetyl-beta-D-glucosaminidase enzymes were significantly lower than the K(m) values of microbial enzymes ingested with their food, further suggesting that the fish efficiently digest soluble components of their detrital diet rather than refractory polysaccharides. Coupled with rapid gut transit and poor cellulose digestibility, the wood-eating catfishes appear to be detritivores reliant on endogenous digestive mechanisms, as are other loricariid catfishes. This stands in contrast to truly "xylivorous" taxa (e.g., beavers, termites), which are reliant on an endosymbiotic community of microorganisms to digest refractory polysaccharides.

  12. Does the hearing sensitivity in thorny catfishes depend on swim bladder morphology?

    Directory of Open Access Journals (Sweden)

    Angelika Zebedin

    Full Text Available BACKGROUND: Thorny catfishes exhibit large variations in swim bladder morphology. These organs are of different sizes, forms and may have simple or branched diverticula. The swim bladder plays an important role in otophysans because it enhances their hearing sensitivity by transmitting sound pressure fluctuations via ossicles to the inner ear. METHODOLOGY/PRINCIPAL FINDINGS: To investigate if a form-function relationship exists, the swim bladder morphology and hearing ability were analyzed in six species. The morphology was quantified by measuring the length, width and height and calculating a standardized swim bladder length (sSBL, which was then used to calculate the relative swim bladder length (rSBL. Hearing was measured using the auditory evoked potential (AEP recording technique. Two species had simple apple-shaped and four species heart-shaped (cordiform bladders. One of the latter species had short unbranched diverticula on the terminal margin, two had a secondary bladder and two had many long, branched diverticula. The rSBL differed significantly between most of the species. All species were able to detect frequencies between 70 Hz and 6 kHz, with lowest thresholds found between 0.5 and 1 kHz (60 dB re 1 µPa. Hearing curves were U-shaped except in Hemidoras morrisi in which it was ramp-like. Mean hearing thresholds of species possessing smaller rSBLs were slightly lower (maximum 8.5 dB than those of species having larger rSBLs. CONCLUSIONS/SIGNIFICANCE: The current findings reveal a relationship between swim bladder form and its function among thorny catfishes. Relatively smaller swim bladders resulted in relatively better hearing. This is in contrast to a prior inter-familial study on catfishes in which species with large unpaired bladders possessed higher sensitivity at higher frequencies than species having tiny paired and encapsulated bladders.

  13. Microglanis pataxo, a new catfish from southern Bahia coastal rivers, northeastern Brazil (Siluriformes: Pseudopimelodidae)

    OpenAIRE

    Sarmento-Soares,Luisa M.; Martins-Pinheiro,Ronaldo F.; Aranda,Arion T.; Chamon,Carine C.

    2006-01-01

    A new pseudopimelodid catfish of the genus Microglanis, collected in small rivers at the lower and middle Peruípe, middle Jucuruçu and Cahy basins, in the southeastern coast of the Bahia state, is described. The new species has a comparatively high number of anal fin proximal radials, 12, shaped as thin tubes, the last one bearing a laminar extension; a pectoral fin spine with a bony point and with a smaller number of serrations on its posterior border; seven pleural ribs and a narrow head wi...

  14. In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

    DEFF Research Database (Denmark)

    Steffensen, John Fleng

    2012-01-01

    of the transparent glass catfish (Kryptopterus bichirris), it is possible to measure near realtime oxygen partial pressure in vivo. We used a commercially-available digital single-lens reflex camera mounted with an optical long pass filter (II = 490 nm) and excited the phosphorescent dye in the microspheres inside...... the fish with externally-mounted blue light emitting diodes (lip = 470 nm) to image the oxygen partial pressure. This method makes it possible to investigate oxygen partial pressures in the vascular system and different tissues of fish without having to insert any probes into the animal. After injection...

  15. Molecular analysis reveals hidden diversity in Zungaro (Siluriformes: Pimelodidade): a genus of giant South American catfish.

    Science.gov (United States)

    Pires, Antonio A; Ramirez, Jorge L; Galetti, Pedro M; Troy, Waldo P; Freitas, Patricia D

    2017-06-01

    The genus Zungaro contains some of the largest catfish in South America. Two valid species are currently recognized: Zungaro jahu, inhabiting the Paraná and Paraguay basins, and Zungaro zungaro, occurring in the Amazonas and Orinoco basins. Analysing Zungaro specimens from the Amazonas, Orinoco, Paraguay and Paraná basins, based on the sequencing of COI and D-loop, we found at least three MOTUs, indicating the existence of hidden diversity within this fish group. Considering the ecological and economic values of this fish, our results are surely welcomed for its conservation, disclosing new findings on its diversity and pointing out the necessity for a detailed taxonomic revision.

  16. Toxicity of Buprofezin on the Survival of Embryo and Larvae of African Catfish, Clarias gariepinus (Bloch)

    OpenAIRE

    Marimuthu, Kasi; Muthu, Narmataa; Xavier, Rathinam; Arockiaraj, Jesu; Rahman, M. Aminur; Subramaniam, Sreeramanan

    2013-01-01

    Buprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus) as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0.05, 0.5, 5, 25, 50 and 100 mg/L) of buprofezin. Each concentration was assessed in five replicates. Eggs were artificially fertilized and 200 eggs and larvae were subjected to a static bath treatme...

  17. Role of various fishmeal ingredients on sangkuriang catfish Clarias sp. growth

    Directory of Open Access Journals (Sweden)

    Nur Bambang Priyo Utomo

    2015-05-01

    Full Text Available ABSTRACT This research was aimed to evaluate the role of fish meal from different raw materials in improving growth performance of sangkuriang catfish Clarias sp. The source of raw materials used were derived from trash fish, salted fish, and head fish eal. Fish reared for 44 days and were given feed test three times per day at 08.00, 12.00, and 16.00. Biomass weight of the experimental fish used were 38.49±1.61 g. The fish reared on the aquarium of 60x50x40 cm3 and by using recirculation system. The results showed that fish meal derived from trash fish provide the best result for feed consumption, daily growth rate, absolute length, biomass, retention of protein, retention of fat, feed efficiency, feed conversion (P>0.05. However, it does not significantly different on the survival of fish (P>0.05. The data showed that the use of trash fish meal resulted on the best growth performance of sangkuriang catfish. It was indicated that the trash-fish meal might be used as the main protein source of sangkuriang catfish feed. Keywords: catfish, growth, at satiation, fish meal  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pakan uji yang menggunakan sumber tepung ikan dari bahan baku berbeda dalam meningkatkan kinerja pertumbuhan lele sangkuriang Clarias sp. Sumber tepung ikan yang digunakan berasal dari ikan rucah, ikan asin, dan kepala ikan, yang memiliki umum digunakan sebagai salah satu bahan baku pakan lokal. Ikan diberi pakan at satiation selama 44 hari dengan frekuensi tiga kali sehari, yaitu pada pukul 08.00, 12.00, dan 16.00. Biomassa rata-rata ikan uji yang digunakan adalah 38,49±1,61 g. Ikan dipelihara dalam akuarium berukuran 60x50x40 cm3 dan dengan menerapkan sistem resirkulasi. Hasil penelitian menunjukkan bahwa tepung ikan yang berasal dari ikan rucah mampu memberikan hasil terbaik dalam meningkatkan kinerja pertumbuhan ikan uji. Hal ini ditunjukkan oleh nilai kinerja pertumbuhan seperti jumlah konsumsi pakan, laju

  18. Effects of vitamin C on percent neurulation, hatch, growth, and survival of hybrid catfish challenged with virulent Edwardsiella ictaluri

    Science.gov (United States)

    Vitamin C (ascorbic acid) is an essential micronutrient involved in several physiological processes such as growth, reproduction, and immune response. Requirements of vitamin C during gamete formation and early life stages of catfish are not known. Ascorbic acid (1 mg/mL/kg BW) was administered in...

  19. Association of Cocaine- and Amphetamine-Regulated Transcript (CART) Messenger RNA Level, Food Intake, and Growth in Channel Catfish

    Science.gov (United States)

    Cocaine-and Amphetamine-Regulated Transcript (CART) is a potent hypothalamic anorectic peptide in mammals and fish. We hypothesized that increased food intake is associated with changes in expression of CART mRNA within the brain of channel catfish. Objectives were to clone the CART gene, examine ...

  20. Draft genome sequence of Pseudomonas mosselii Gil3, isolated from catfish and antagonistic against hypervirulent Aeromonas hydrophila

    Science.gov (United States)

    Pseudomonas mosselii Gil3 was isolated from a catfish that survived from lethal challenge with hypervirulent Aeromonas hydrophila (vAh). When assayed in vitro, the bacterium showed antagonism against vAh. Sequence analysis revealed that the genome of P. mosselii Gil3 encodes numerous aromatic metabo...

  1. Partial and total fish meal replacement by agricultural products in the diets improve sperm quality in African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Nyina-wamwiza, L.; Milla, S.; Pierrard, M.A.; Rurangwa, E.; Mandiki, S.N.M.; Look, van K.J.W.; Kestemont, P.

    2012-01-01

    This study investigated the long-term effects of total and partial replacement of dietary fish meal (FM) by a mixture of agricultural products on sperm quality of African catfish Clarias gariepinus. Four isonitrogenous and isoenergetic diets were formulated containing graded levels of either 50% FM

  2. Effects of Ichthyophthirius multifiliis parasitism on the survival, hematology and bacterial load in channel catfish previously exposed to Edwardsiella ictaluri

    Science.gov (United States)

    The effect of Ichthyophthirius multifiliis (Ich) parasitism on survival, hematology and bacterial load in channel catfish, Ictalurus punctatus, previously exposed to Edwardsiella ictaluri was studied. Fish were exposed to E. ictaluri one day prior to Ich in the following treatments: 1)- infected by...

  3. HPLC Determination and MS Confirmation of Malachite Green, Gentian Violet, and Their Leuco Metabolites in Catfish Muscle

    Science.gov (United States)

    Residues of malachite green (MG), gentian violet (GV), and their leuco metabolites in catfish muscle were individually determined by HPLC using visible and fluorescence detectors. This detection scheme obviated a PbO2 column that converts leuco forms to chromatic forms for visible detection, thus el...

  4. Comparative effects of copper sulfate or potassium permanganate on channel catfish concurrently infected with Flavobacterium columnare and Ichthyobodo necator

    Science.gov (United States)

    An opportunistic study was conducted to determine the effects of two chemical therapeutants on channel catfish (CCF) Ictalurus punctatus concurrently infected Flavobacterium columnare and Ichthyobodo necator. Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were investigated for their abil...

  5. Effect of Pre-cooking and Addition of Phosphate on the Quality of Microwave Cooked Catfish Fillets

    Science.gov (United States)

    In the US market place there are many examples of precooked poultry products designed to be reheated in a microwave oven and to a lesser extent fish products such as tilapia. However, there are few US catfish products designed to be microwave cooked or reheated in the market place. The first objecti...

  6. Genome-wide identification, characterization and phylogenetic analysis of 50 catfish ATP-binding cassette (ABC) transporter genes.

    Science.gov (United States)

    Liu, Shikai; Li, Qi; Liu, Zhanjiang

    2013-01-01

    Although a large set of full-length transcripts was recently assembled in catfish, annotation of large gene families, especially those with duplications, is still a great challenge. Most often, complexities in annotation cause mis-identification and thereby much confusion in the scientific literature. As such, detailed phylogenetic analysis and/or orthology analysis are required for annotation of genes involved in gene families. The ATP-binding cassette (ABC) transporter gene superfamily is a large gene family that encodes membrane proteins that transport a diverse set of substrates across membranes, playing important roles in protecting organisms from diverse environment. In this work, we identified a set of 50 ABC transporters in catfish genome. Phylogenetic analysis allowed their identification and annotation into seven subfamilies, including 9 ABCA genes, 12 ABCB genes, 12 ABCC genes, 5 ABCD genes, 2 ABCE genes, 4 ABCF genes and 6 ABCG genes. Most ABC transporters are conserved among vertebrates, though cases of recent gene duplications and gene losses do exist. Gene duplications in catfish were found for ABCA1, ABCB3, ABCB6, ABCC5, ABCD3, ABCE1, ABCF2 and ABCG2. The whole set of catfish ABC transporters provide the essential genomic resources for future biochemical, toxicological and physiological studies of ABC drug efflux transporters. The establishment of orthologies should allow functional inferences with the information from model species, though the function of lineage-specific genes can be distinct because of specific living environment with different selection pressure.

  7. Rapid screening of oxytetracycline residue in catfish muscle by dispersive liquid-liquid microextraction and europium-sensitized luminescence

    Science.gov (United States)

    Oxytetracycline (OTC) residue in catfish muscle was screened by dispersive liquid-liquid microextraction (DLLME) and europium-sensitized luminescence (ESL). After extraction in EDTA, HCl, and acetonitrile, cleanup was carried out by DLLME, and ESL was measured at microgram = 385 nm and wavelength = ...

  8. Can reduced stocking rates and natural forage utilization produce market sized catfish from fingerlings in one growing season

    Science.gov (United States)

    The purpose of this study was to determine the feasibility of growing marketable channel catfish Ictalurus punctatus from pond-run fingerlings (15.9 g/fish) using low stocking densities (7,413 or 14,826 fish/ha) and electrified bug lights to enhance natural forage available to fish. Even at low sto...

  9. Effect of supplemental taurine on juvenile channel catfish Ictalurus punctatus growth and survival after challenge with Edwardsiella ictaluri

    Science.gov (United States)

    Juvenile channel catfish, Ictalurus punctatus, were fed a basal diet that contained major protein (soybean meal, cottonseed meal) and energy (ground corn grain) ingredients that were derived from plant sources. Plant-source ingredients are considered to be low (taurine content. In add...

  10. Laboratory investigation of daily food intake and gut evacuation in larvae of African catfish Clarias gariepinus under different feeding conditions

    NARCIS (Netherlands)

    García-Ortega, A.; Verreth, J.A.J.; Vermis, K.; Nelis, H.J.; Sorgeloos, P.; Verstegen, M.W.A.

    2010-01-01

    Abstract Temporary accumulation of ascorbic acid 2-sulfate (AAS) was measured to estimate food intake and gut evacuation in larvae of African catfish. Fish larvae were fed decapsulated cysts of Artemia containing AAS. In a first experiment it was found that no biosynthesis of AAS occurs in the

  11. Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptome.

    Directory of Open Access Journals (Sweden)

    Fanyue Sun

    Full Text Available BACKGROUND: Catfish has a male-heterogametic (XY sex determination system, but genes involved in gonadogenesis, spermatogenesis, testicular determination, and sex determination are poorly understood. As a first step of understanding the transcriptome of the testis, here, we conducted RNA-Seq analysis using high throughput Illumina sequencing. METHODOLOGY/PRINCIPAL FINDINGS: A total of 269.6 million high quality reads were assembled into 193,462 contigs with a N50 length of 806 bp. Of these contigs, 67,923 contigs had hits to a set of 25,307 unigenes, including 167 unique genes that had not been previously identified in catfish. A meta-analysis of expressed genes in the testis and in the gynogen (double haploid female allowed the identification of 5,450 genes that are preferentially expressed in the testis, providing a pool of putative male-biased genes. Gene ontology and annotation analysis suggested that many of these male-biased genes were involved in gonadogenesis, spermatogenesis, testicular determination, gametogenesis, gonad differentiation, and possibly sex determination. CONCLUSION/SIGNIFICANCE: We provide the first transcriptome-level analysis of the catfish testis. Our analysis would lay the basis for sequential follow-up studies of genes involved in sex determination and differentiation in catfish.

  12. Head-only electrical stunning and bleeding of African catfish (Clarias gariepinus): assessment of loss of consciousness

    NARCIS (Netherlands)

    Lambooij, E.; Kloosterboer, R.J.; Gerritzen, M.A.; Vis, van de J.W.

    2004-01-01

    The objective was to evaluate the welfare implications of electrical stunning prior to gill-cutting of farmed African catfish as an alternative to live chilling in combination with gutting. Electroencephalogram (EEG) and electrocardiogram (ECG) recordings, in combination with observation of

  13. Retention of health-related beneficial components during household preparation of selenium-enriched African catfish (Clarias gariepinus) fillets

    NARCIS (Netherlands)

    Mierke-Klemeyer, S.; Larsen, R.; Oehlenschlaeger, J.; Nunes, M.L.; Schram, E.; Luten, J.B.

    2008-01-01

    Industrial processing and heat treatment of fish muscle generally lead to losses of water-soluble components, some of which may have beneficial health effects. The aim of this work was to determine the retention of taurine, selenium and n-3 polyunsaturated fatty acids when preparing African catfish

  14. Effect of refrigeration time on the lipid oxidation and fatty acid profiles of catfish (Arius maculatus) commercialized in Cameroon

    International Nuclear Information System (INIS)

    Tenyang, N.; Womeni, H.M.; Tiencheu, B.; Villeneuve, P.; Linder, M.

    2017-01-01

    The effects of refrigeration at 4 °C during 9 days on the quality and stability of catfish oil were evaluated using a change in fatty acid composition by gas chromatography (GC), commonly used analytical indexes (acid and peroxide values), and analysis by Fourier transform infrared (FTIR) spectroscopy. The results revealed that lipid deterioration, hydrolysis and oxidation occurred throughout the cold storage (4 °C). Refrigeration induced the lipolysis of triglycerides by lipases and phospholipases. It also affected the fatty acids composition of the catfish. The progressive loss of unsaturation was monitored by the decrease in the absorbance band at 3012 cm−1on FTIR spectra and the lowest value was observed in the catfish muscle at 9 days of refrigeration. Eicosapentaenoic C20:5ω3 (EPA) and docosahexaenoic C22:6ω3 (DHA) acids were the polyunsaturated fatty acids most affected during refrigeration. Refrigeration for less than 5 days was found to be the best conditions for the preservation of the catfish. [es

  15. Seasonal changes in steroid metabolism in the male reproductive organ-system of the African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Resink, J.W.; Schoonen, W.G.E.J.; Hurk, R. van den; Viveen, W.J.A.R.; Lambert, J.G.D.

    1987-01-01

    Steroid and steroid glucuronide synthesis in feral male African catfish was investigated in vitro by incubating testes with [3H]-pregnenolone and seminal vesicles with [3H]-androstenedione. In testes, the capacity to form progestins, androgens, especially 11-oxygenated ones, and steroid glucuronides

  16. Extensive jaw mobility in suckermouth armored catfishes (Loricariidae): a morphological and kinematic analysis of substrate scraping mode of feeding.

    Science.gov (United States)

    Adriaens, Dominique; Geerinckx, Tom; Vlassenbroeck, Jelle; Van Hoorebeke, Luc; Herrel, Anthony

    2009-01-01

    Loricariidae, or suckermouth armored catfishes, possess upper and lower jaws that are ventrally oriented and that bear teeth that touch the substrate from which algae and other food items are scraped. The ventral orientation and the highly specialized morphology of the jaws, characterized by protrusible upper jaws and left-right decoupled lower jaws, are observed in Pterygoplichthys disjunctivus, the species investigated here. Kinematic data of the scraping feeding movements, obtained by external high-speed and x-ray recordings, are used to quantify jaw movement, especially to test for upper jaw mobility and versatility during substrate scraping. Our results show that the mobility of the jaws is indeed high compared with what is standard for catfishes. The upper jaw's ability to perform a substantial degree of rostrocaudal movement is quite unique for catfishes. The ventromedially oriented lower jaws, with the teeth and the coronoid process at opposite sides, display an extensive mobility: they rotate around the suspensorial articulation and around their longitudinal axis, resulting in an extended scraping movement and thereby covering a large surface area. The lower jaws also show a left-right asymmetry in their movements during scraping. Thus, our results suggest that the extreme morphological specializations of the jaws in loricariid catfishes are linked to an increased mobility and functional versatility, allowing these animals to efficiently scrape algae from substrates with irregular surfaces.

  17. In vivo activity of native GnRHs and their analogues on ovulation in the American catfish, Clarias gariepinus (Burchell)

    Czech Academy of Sciences Publication Activity Database

    Szabó, T.; Radics, F.; Barth, Tomislav; Horváth, L.

    2007-01-01

    Roč. 38, č. 2 (2007), s. 140-146 ISSN 1355-557X R&D Projects: GA MZe QF3028; GA MZe QF3029 Institutional research plan: CEZ:AV0Z40550506 Keywords : GnRH * ovulation * African catfish Subject RIV: CC - Organic Chemistry Impact factor: 1.067, year: 2007

  18. Dietary Carnitine maintains energy reserves and delays fatigue of exercised African catfish (Clarias Gariepinus) fed high fat diets

    NARCIS (Netherlands)

    Ozorio, R.; Ginneken, van V.J.T.; Thillart, van den G.; Verstegen, M.W.A.; Verreth, J.A.J.

    2005-01-01

    Lipids, together with proteins, are traditionally considered as primary fuels during aerobic swimming. The effects of dietary fat and carnitine supplements and exercise on the energy metabolism of juvenile fish were investigated. One hundred African catfish (Clarias gariepinus) were fed four

  19. Nitric oxide permits hypoxia-induced lymphatic perfusion by controlling arterial-lymphatic conduits in zebrafish and glass catfish

    DEFF Research Database (Denmark)

    Dahl Ejby Jensen, Lasse; Cao, Renhai; Hedlund, Eva-Maria

    2009-01-01

    and lymphatic perfusion. Here we show in the adult zebrafish and glass catfish (Kryptopterus bicirrhis) that blood-lymphatic conduits directly connect arterial vessels to the lymphatic system. Under hypoxic conditions, arterial-lymphatic conduits (ALCs) became highly dilated and linearized by NO...

  20. Steroidogenesis in the testes and seminal vesicles of spawning and non-spawning African catfish, Clarias gariepinus

    NARCIS (Netherlands)

    Schoonen, W.G.E.J.; Granneman, J.C.M.; Lambert, J.G.D.; Oordt, P.G.W.J. van

    1987-01-01

    The in vitro biosynthesis of steroids was studied in testes as well as seminal vesicles of non-spawning pond and spawning feral African catfish, collected during the breeding season. In testes of non-spawners the conversion of [3H]-pregnenolone was directed towards 11-oxygenated androgens and

  1. Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: A negative regulator of immunoglobulin gene transcription?

    NARCIS (Netherlands)

    Lennard, M.L.; Hikima, J.I.; Ross, D.A.; Kruiswijk, C.P.; Wilson, M.R.; Miller, N.W.; Warr, G.W.

    2007-01-01

    Background - The enhancer (E¿3') of the immunoglobulin heavy chain locus (IGH) of the channel catfish (Ictalurus punctatus) has been well characterized. The functional core region consists of two variant Oct transcription factor binding octamer motifs and one E-protein binding ¿E5 site. An

  2. Effects of acute stress on aggression and the cortisol response in the African sharptooth catfish Clarias gariepinus

    NARCIS (Netherlands)

    Manuel, R.; Boerrigter, J.G.J.; Cloosterman, M.; Gorissen, M.; Flik, G.; Bos, van den R.; Vis, van de H.

    2016-01-01

    African sharptooth catfish Clarias gariepinus were housed under continuous dim light (1lx) or 12L:12D (350-0lx) cycles. The number of skin lesions, as indicator of aggressive acts, and plasma cortisol levels, as indicator of stress-axis activity, were measured at baseline as well as following a

  3. Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp.

    Directory of Open Access Journals (Sweden)

    Enita Romasni Turnip

    2018-05-01

    Full Text Available ABSTRACT This study aimed to select lactic acid bacteria (LAB as a potential probiotic that producing anti‒microbial compounds in order to treat motile aeromonads septicemia diseases caused by Aeromonas hydrophila on catfish Clarias sp. and evaluated its performance on gnotobiotic catfish. The in vitro assay was done to select several LAB isolates based on antagonistic activity against pathogenic bacteria. The selected isolate was tested in vivo to observe their ability to improve growth performances of catfish. The study was conducted with five treatments consists of K‒ (normal catfish without addition probiotic, without challenge test, K+ (normal catfish without addition of probiotic, with challenge test, Np (normal catfish with addition of probiotic and challenge test, G (gnoto catfish without addition of probiotic, with challenge test, and Gp (gnoto catfish with addition of probiotic and challenge test. The results showed that the addition of Pediococcus pentosaceus E2211 as selected probiotic could increase survival rate, specific growth rate, and immune response towards infection of A. hydrophila. The best survival rate after challenge test was obtained in Np and Gp treatments (88.46%, followed by G treatment (65.38%, while the K+ was only 53.84%. The conclusion of this study was P. pentosaceus E2211 potentially used as a probiotic candidate for normal and gnotobiotic catfish. The presence of normal microflora with P. pentosaceus E2211 in Np treatment showed the best probiotic performance with daily growth rate 3.28%, feed conversion ratio 1.79, and total intestinal bacteria reached 108 CFU/mL significantly different from other treatments (P<0.05.Keywords: Aeromonas hydrophila, catfish, LAB, probiotic, screening  ABSTRAKTujuan penelitian ini adalah menyeleksi bakteri asam laktat (BAL sebagai probiotik potensial penghasil senyawa antimikrob guna menanggulangi penyakit motile aeromonad septicemia akibat Aeromonas hydrophila pada ikan

  4. Gene expression analysis in gonads and brain of catfish Clarias batrachus after the exposure of malathion.

    Science.gov (United States)

    Prathibha, Y; Murugananthkumar, R; Rajakumar, A; Laldinsangi, C; Sudhakumari, C C; Mamta, S K; Dutta-Gupta, A; Senthilkumaran, B

    2014-04-01

    Pesticides like malathion have the potential to disrupt development and reproduction of aquatic organisms including fishes. To investigate the likely consequences of malathion exposure at low doses in juvenile catfish, Clarias batrachus, we studied the expression pattern of genes encoding certain transcription factors, activin A, sex steroid or orphan nuclear receptors and steroidogenic enzymes which are known to be involved in gonadal development along with histological changes. To compare further, we also analyzed certain brain specific genes related to gonadal axis. Fifty days post hatch catfish fingerlings were exposed continuously to 1 and 10 µg/L of malathion for 21 days. Results from these experiments indicated that transcript levels of various genes were altered by the treatments, which may further affect the gonadal development either directly or indirectly through brain. Histological analysis revealed slow progression of spermatogenesis in testis, while in ovary, the oil droplet oocytes were found to be higher after treatment (10 µg/L). Our findings revealed that the exposure of malathion, even at low doses, hinder or modulate early gonadal development differentially by targeting gene expression pattern of transcription factors, activin A, sex steroid or orphan nuclear receptors and steroidogenic enzymes with an evidence on histological changes. Further, some of the genes showed differential expression at the level of brain in male and female sex after the exposure of malathion. Copyright © 2014. Published by Elsevier Inc.

  5. Intestinal synthesis and absorption of vitamin B-12 in channel catfish

    International Nuclear Information System (INIS)

    Limsuwan, T.; Lovell, R.T.

    1981-01-01

    A feeding experiment conducted in a controlled environment and using a vitamin B12-deficient, but otherwise nutritionally complete, purified diet revealed that intestinal microorganisms in channel catfish synthesized approximately 1.4 ng of vitamin B12 per gram of bodyweight per day. Removal of cobalt from the diet or supplementation with an antibiotic (succinylsulfathiazole) significantly reduced the rate of intestinal synthesis and liver stores of vitamin B12. Radiolabeled vitamin B12 in the blood, liver, kidneys, and spleen of fish fed 60Co in the diet indicated that the intestinally synthesized vitamin was absorbed by the fish. The primary route of absorption was directly from the digestive tract into the blood because coprophagy was prevented in the rearing aquariums and the amount of vitamin B12 dissolved in the aquarium water was too low for gill absorption. Dietary supplementation of vitamin B12 was not necessary for normal growth and erythrocyte formation in channel catfish in a 24-week feeding period. A longer period, however, may have caused a vitamin deficiency since liver-stored vitamin B 12 decreased between the 2nd and 24th weeks

  6. Elimination of {sup 137}Cs from Japanese Catfish Acutely Contaminated by Labelled Food

    Energy Technology Data Exchange (ETDEWEB)

    Malek, M. A. [Atomic Energy Center, Dhaka (Bangladesh); Nakahara, M. [Laboratory for Radioecology, National Institute of Radiological Sciences, Isozaki, Hitachinaka City, Ibaraki-ken (Japan)

    2013-07-15

    The effective half-life of {sup 137}Cs in acutely contaminated Japanese catfish was determined. The fish were fed with fish meal incorporated with {sup 137}CsCl and then released into aquaria. To assess the {sup 137}Cs activity retained in the fish body, the whole body radioactivity of each fish was measured at regular intervals for up to 157 days. The data were plotted with relative counts on the y-axis and corresponding elapsed time on the x-axis. Relative count plotted against elapsed time was found to consist of three components, namely the first fast, second fast and the slow component. The true elimination curves of the first components were calculated by the peeling off method. The effective half-life of the fast components was determined from the slope of the true curves. The effective half-life of {sup 137}Cs for the first fast, second fast and the slow component in Japanese catfish was found to be 1.27, 5.76 and 251 days, respectively. (author)

  7. Convergence among cave catfishes: long-branch attraction and a Bayesian relative rates test.

    Science.gov (United States)

    Wilcox, T P; García de León, F J; Hendrickson, D A; Hillis, D M

    2004-06-01

    Convergence has long been of interest to evolutionary biologists. Cave organisms appear to be ideal candidates for studying convergence in morphological, physiological, and developmental traits. Here we report apparent convergence in two cave-catfishes that were described on morphological grounds as congeners: Prietella phreatophila and Prietella lundbergi. We collected mitochondrial DNA sequence data from 10 species of catfishes, representing five of the seven genera in Ictaluridae, as well as seven species from a broad range of siluriform outgroups. Analysis of the sequence data under parsimony supports a monophyletic Prietella. However, both maximum-likelihood and Bayesian analyses support polyphyly of the genus, with P. lundbergi sister to Ictalurus and P. phreatophila sister to Ameiurus. The topological difference between parsimony and the other methods appears to result from long-branch attraction between the Prietella species. Similarly, the sequence data do not support several other relationships within Ictaluridae supported by morphology. We develop a new Bayesian method for examining variation in molecular rates of evolution across a phylogeny.

  8. Effect of Salinity Adaptation Technique on Survival and Growth Rate of Patin Catfish, Pangasius sp.

    Directory of Open Access Journals (Sweden)

    K. Nirmala

    2007-01-01

    Full Text Available This study was carried out to determine the effect of salinity adaptation techniques on growth and survival of patin catfish Pangasius sp. fry.  Fry of 1.5-2.0 inch in length were reared in the water with different of the initial salinity of 1, 2, 3, 4 and 5 ppt.  Salinity was then daily increased by duplicated the initial water salinity until fish died.  The results of study showed that fry could survive by initial salinity adaptation of 1 ppt and then increasing the salinity by 1 ppt/day to reach 27 ppt.  In the other treatments, all fry died after the salinity reach 18-25 ppt. Keywords: patin catfish, Pangasius, adaptation, salinity   ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh teknik adaptasi salinitas terhadap pertumbuhan dan kelangsungan hidup benih ikan patin Pangasius sp.  Benih patin ukuran 1,5-2 inci dipelihara pada salinitas awal berbeda, yaitu 1, 2, 3, 4 dan 5 ppt. Salinitas air pemeliharaan ditingkatkan kelipatan dari salinitas awal setiap hari hingga ikan mati.  Hasil penelitian menunjukkan bahwa adaptasi salinitas awal 1 ppt dan peningkatan sebesar 1ppt/hari menyebabkan ikan dapat bertahan hidup sampai pada salinitas 27 ppt. Pada perlakuan lainnya, benih ikan mengalami kematian masal ketika salinitas mencapai 18-25 ppt. Kata kunci: ikan patin, Pangasius, adaptasi, salinitas

  9. Preliminary study on element mass fraction determination on catfish samples from Paraguay

    International Nuclear Information System (INIS)

    Moreira, Edson G.; Catharino, Marilia G.M.; Vasconcellos, Marina B.A.; Frutos, Sixto A.; Insaurralde, Mario S.

    2013-01-01

    South American catfish (Pseudoplatystoma), commonly known in Spanish as atigrado or surubi and in Portuguese as surubim or pintado is a large fish that typically reaches 1 m long and weighs 60 kg to 80 kg and may be found at the basins of the Amazon, the Sao Francisco and de la Plata rivers, usually in riverbeds and deep wells. Being a much appreciated fish for human consumption, it is quite sought after by fishermen who have been contributing to the reduction of the stocks. This fact attracted the attention of the Paraguayan authorities to the point of imposing restrictions to free fishing and commercialization. This study aims to assist the conservation efforts towards this fish by investigating its exposure to possible pollutants. Preliminary results on element determination on six samples of catfish from Paraguayan rivers are presented. Cs, Co, Fe, Se and Zn were determined by applying an Instrumental Neutron Activation Analysis method. While these element levels were lower than the legislation for human consumption, the elements As, Cr e La were not detected in the samples as they are below the detection limit of the method employed. Atomic Absorption Spectrometry was used to investigate the presence of Cd, Hg and Pb in the samples. Hg was detected in the samples while Cd and Pb were below the detection limit of the method. (author)

  10. Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus)

    Science.gov (United States)

    Sarker, Mohamed Zaidul Islam; Selamat, Jinap; Habib, Abu Sayem Md. Ahsan; Ferdosh, Sahena; Akanda, Mohamed Jahurul Haque; Jaffri, Juliana Mohamed

    2012-01-01

    Fish oil was extracted from the viscera of African Catfish using supercritical carbon dioxide (SC-CO2). A Central Composite Design of Response Surface methodology (RSM) was employed to optimize the SC-CO2 extraction parameters. The oil yield (Y) as response variable was executed against the four independent variables, namely pressure, temperature, flow rate and soaking time. The oil yield varied with the linear, quadratic and interaction of pressure, temperature, flow rate and soaking time. Optimum points were observed within the variables of temperature from 35 °C to 80 °C, pressure from 10 MPa to 40 MPa, flow rate from 1 mL/min to 3 mL/min and soaking time from 1 h to 4 h. However, the extraction parameters were found to be optimized at temperature 57.5 °C, pressure 40 MPa, flow rate 2.0 mL/min and soaking time 2.5 h. At this optimized condition, the highest oil yields were found to be 67.0% (g oil/100 g sample on dry basis) in the viscera of catfish which was reasonable to the yields of 78.0% extracted using the Soxhlet method. PMID:23109854

  11. Estimates of population genetic diversity in brown bullhead catfish by DNA fingerprinting

    Energy Technology Data Exchange (ETDEWEB)

    Roth, A.C.; Wessendarp, T.K.; Gordon, D.A.; Smith, M.K. [Environmental Protection Agency, Cincinnati, OH (United States); Lattier, D.L. [Oak Ridge Inst. for Science and Education, Cincinnati, OH (United States); Hertzberg, V.; Leonard, A. [Univ. of Cincinnati, OH (United States). Dept. of Environmental Health

    1994-12-31

    Estimates of population genetic diversity may be a sensitive indicator of environmental impact, since limiting the effective breeding population by any means will result in loss of some variant genotypes, as has been demonstrated by allozyme analysis. DNA fingerprinting techniques are also coming into use for population analyses, and the authors chose to apply fingerprinting analysis three populations of brown bullhead catfish collected in Northern Ohio. DNA was isolated from the red blood cells of individual fish. Purified DNAs were digested with EcoR1 restriction enzyme; the digests were then sized on a 1% agarose gel, transferred to nylon membranes and probed with a radiolabeled M13 probe using the Westneat hybridization protocol (Southern blotting). This method effects fragments containing VNTR (variable number of tandem repeat) sequences complementary to the M13, which are highly variable among individual catfish. Hybridized bands were visualized by a Molecular Dynamics phosphorimager and recorded and analyzed with its proprietary Imagequant image analysis program, Excel and SAS. A total of 10 variable bands were identified and their presence or absence scored in each individual. These data were analyzed to determine between and within-population similarity indices as well as population heterozygosity and genetic diversity measures.

  12. Antibacterial efficacies of some plant extracts against Aeromonas and Pseudomonas diseases of farmed catfish (Heterobranchus longifilis

    Directory of Open Access Journals (Sweden)

    Albert P. Ekanem

    2011-11-01

    Full Text Available Aeromonas and Pseudomonas diseases are responsible for mortalities of some farmed catfish in Nigeria. The objective of the study is to investigate the efficacies of extracts of some plants against Aeromonas and Pseudomonas diseases of Heterobranchus longifilis. Ethanol extracts of Phyllanthus amarus, Allium sativum, Artemisia annua, Citrus limon, Moringa oleifera, Allium cepa and Azadirachta indica were tested against Aeromonas hydrophila and Pseudomonas flourescens of H. longifilis by disc diffusion assay. Extracts of P. amarus, A. sativum, A. annua and C. limon were significantly (P<0.05 more sensitive to A. hydrophila and P. flourescens than M. oleifera, A. cepa and A. indica which were effective against P. flourescens. Minimum inhibitory concentrations (MIC of the extracts were 25mg/ml for P. amarus and A. annua; 25 and 100mg/ml for C. lemon and A. cepa respectively and 50mg/ml for A. indica.  Alkaloid was demonstrated in all plants except A. annua by qualitative methods. Moderate amount (++ of cardiac glycosides was demonstrated in A. sativum, M. oleifera and P. amarus. Saponin (+++ was present in M. oleifera and A. indica while, tannin (++ was present in M. oleifera, P. amarus and A. indica respectively. Phlobatanins and Anthraquinones (++ were present in P. amarus and M. oleifera respectively.  Extracts of aforementioned plants have potentials as therapy against Aeromonas hydrophila and Pseudomonas flourescens of farmed catfish.

  13. First record of Trichodina heterodentata (Ciliophora: Trichodinidae from channel catfish, Ictalurus punctatus cultivated in Brazil

    Directory of Open Access Journals (Sweden)

    ML. Martins

    Full Text Available This study characterises morphologically Trichodina heterodentata Duncan, 1977 from channel catfish, Ictalurus punctatus (Rafinesque, 1818 in the State of Santa Catarina, Brazil. Body and gill smears were air-dried at room temperature, impregnated with silver nitrate and/or stained with Giemsa. Ten characteristics were selected to compare the present material with other morphological characterisations of T. heterodentata. Prevalence rate was 100%, mean intensity 89,333.70 (3,125 to 299,100 parasites per host. Trichodina heterodentata was considered medium-sized trichodinid with mean body diameter 59.4 ± 8.5 μm, denticulate ring 38.5 ± 4.5 μm, adhesive disc 60.2 ± 6.7 μm diameter and 24.4 ± 1.6 denticles. In relation to previous reports of T. heterodentata this material resembles in 90% of the analysed characters. This work confirms the biometrical variation that exists in the different populations of T. heterodentata. A list of hosts and comparative measurements of T. heterodentata are presented and the channel catfish is considered a new host.

  14. Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch.

    Directory of Open Access Journals (Sweden)

    Kasi Marimuthu

    Full Text Available Buprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0.05, 0.5, 5, 25, 50 and 100 mg/L of buprofezin. Each concentration was assessed in five replicates. Eggs were artificially fertilized and 200 eggs and larvae were subjected to a static bath treatment for all the concentrations. The mortality of embryos was significantly increased with increasing buprofezin concentrations from 5 to 100 mg/L (p0.05 in the LC50 values obtained at 24 and 48 h exposure times. Malformations were observed when the embryos and larvae exposed to more than 5 mg/L. The results emerged from the study suggest that even the low concentration (5 mg/L of buprofezin in the aquatic environment may have adverse effect on the early embryonic and larval development of African catfish.

  15. Preliminary study on element mass fraction determination on catfish samples from Paraguay

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Edson G.; Catharino, Marilia G.M.; Vasconcellos, Marina B.A., E-mail: emoreira@ipen.br, E-mail: mbvascon@ipen.br, E-mail: mariliasemmler@uol.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Frutos, Sixto A.; Insaurralde, Mario S., E-mail: tony8013@hotmail.com, E-mail: insaurraldemar9@hotmail.com [Universidad Nacional de Asuncion (FCV/UNA), San Lorenzo (Paraguay). Facultad de Ciencias Veterinarias. Departamento de Pesca y Acuicultura

    2013-07-01

    South American catfish (Pseudoplatystoma), commonly known in Spanish as atigrado or surubi and in Portuguese as surubim or pintado is a large fish that typically reaches 1 m long and weighs 60 kg to 80 kg and may be found at the basins of the Amazon, the Sao Francisco and de la Plata rivers, usually in riverbeds and deep wells. Being a much appreciated fish for human consumption, it is quite sought after by fishermen who have been contributing to the reduction of the stocks. This fact attracted the attention of the Paraguayan authorities to the point of imposing restrictions to free fishing and commercialization. This study aims to assist the conservation efforts towards this fish by investigating its exposure to possible pollutants. Preliminary results on element determination on six samples of catfish from Paraguayan rivers are presented. Cs, Co, Fe, Se and Zn were determined by applying an Instrumental Neutron Activation Analysis method. While these element levels were lower than the legislation for human consumption, the elements As, Cr e La were not detected in the samples as they are below the detection limit of the method employed. Atomic Absorption Spectrometry was used to investigate the presence of Cd, Hg and Pb in the samples. Hg was detected in the samples while Cd and Pb were below the detection limit of the method. (author)

  16. Molecular phylogeny of the highly diversified catfish subfamily Loricariinae (Siluriformes, Loricariidae) reveals incongruences with morphological classification.

    Science.gov (United States)

    Covain, Raphaël; Fisch-Muller, Sonia; Oliveira, Claudio; Mol, Jan H; Montoya-Burgos, Juan I; Dray, Stéphane

    2016-01-01

    The Loricariinae belong to the Neotropical mailed catfish family Loricariidae, the most species-rich catfish family. Among loricariids, members of the Loricariinae are united by a long and flattened caudal peduncle and the absence of an adipose fin. Despite numerous studies of the Loricariidae, there is no comprehensive phylogeny of this morphologically highly diversified subfamily. To fill this gap, we present a molecular phylogeny of this group, including 350 representatives, based on the analysis of mitochondrial and nuclear genes (8426 positions). The resulting phylogeny indicates that Loricariinae are distributed into two sister tribes: Harttiini and Loricariini. The Harttiini tribe, as classically defined, constitutes a paraphyletic assemblage and is here restricted to the three genera Harttia, Cteniloricaria, and Harttiella. Two subtribes are distinguished within Loricariini: Farlowellina and Loricariina. Within Farlowellina, the nominal genus formed a paraphyletic group, as did Sturisoma and Sturisomatichthys. Within Loricariina, Loricaria, Crossoloricaria, and Apistoloricaria are also paraphyletic. To solve these issues, and given the lack of clear morphological diagnostic features, we propose here to synonymize several genera (Quiritixys with Harttia; East Andean members of Crossoloricaria, and Apistoloricaria with Rhadinoloricaria; Ixinandria, Hemiloricaria, Fonchiiichthys, and Leliella with Rineloricaria), to restrict others (Crossoloricaria, and Sturisomatichthys to the West Andean members, and Sturisoma to the East Andean species), and to revalidate the genus Proloricaria. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Parasites of two coexisting invasive sailfin catfishes (Siluriformes: Loricariidae in a tropical region of Mexico

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    María Amparo Rodríguez-Santiago

    Full Text Available ABSTRACT Currently many species of Amazon sailfin catfishes (Loricariidae have been introduced to wild environments outside their native range. There is, however, little knowledge about their role as vectors of parasites that can infect native fish or even humans through its consumption. The aim of the present study was to determine the parasitic fauna of the invasive sailfin catfish species Pterygoplichthys pardalis (leopard pleco and P. disjunctivus (vermiculated pleco from freshwater systems in the southeast of Mexico. Four ectoparasite species were found in P. pardalis (1 protozoan: Ichthyophthirius multifiliis ; 2 monogeneans: Urocleidoides vaginoclastrum and Heteropriapulus heterotylus ; 1 digenean: Clinostomum sp., and only one in Heteropriapulus disjunctivus (H. heterotylus . No endoparasites were found. Ichthyophthirius multifiliis , U. vaginoclaustrum and Clinostomum sp. , were considered as rare species (prevalence <5% since they were found in a single individual of P. pardalis . H. heterotylus was the only species shared among both host species and it occurs throughout the year. This monogenean species represents 96% of total parasites recorded in P. pardalis and 100% in P. disjunctivus. Monthly values of prevalence, intensity and abundance of H. heterotylus in both host species showed important intra-annual variations, but not differ significantly between both hosts.

  18. Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus

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    Mohamed Zaidul Islam Sarker

    2012-09-01

    Full Text Available Fish oil was extracted from the viscera of African Catfish using supercritical carbon dioxide (SC-CO2. A Central Composite Design of Response Surface methodology (RSM was employed to optimize the SC-CO2 extraction parameters. The oil yield (Y as response variable was executed against the four independent variables, namely pressure, temperature, flow rate and soaking time. The oil yield varied with the linear, quadratic and interaction of pressure, temperature, flow rate and soaking time. Optimum points were observed within the variables of temperature from 35 °C to 80 °C, pressure from 10 MPa to 40 MPa, flow rate from 1 mL/min to 3 mL/min and soaking time from 1 h to 4 h. However, the extraction parameters were found to be optimized at temperature 57.5 °C, pressure 40 MPa, flow rate 2.0 mL/min and soaking time 2.5 h. At this optimized condition, the highest oil yields were found to be 67.0% (g oil/100 g sample on dry basis in the viscera of catfish which was reasonable to the yields of 78.0% extracted using the Soxhlet method.

  19. Genetic structure in the Amazonian catfish Brachyplatystoma rousseauxii: influence of life history strategies.

    Science.gov (United States)

    Carvajal-Vallejos, F M; Duponchelle, F; Desmarais, E; Cerqueira, F; Querouil, S; Nuñez, J; García, C; Renno, J-F

    2014-08-01

    The Dorado or Plateado (Gilded catfish) Brachyplatystoma rousseauxii (Pimelodidae, Siluriformes) is a commercially valuable migratory catfish performing the largest migration in freshwaters: from the Amazonian headwaters in the Andean foothills (breeding area) to the Amazon estuary (nursery area). In spite of its importance to inform management and conservation efforts, the genetic variability of this species has only recently begun to be studied. The aim of the present work was to determine the population genetic structure of B. rousseauxii in two regions: the Upper Madera Basin (five locations in the Bolivian Amazon) and the Western Amazon Basin (one regional sample from the Uyucalí-Napo-Marañon-Amazon basin, Peru). Length polymorphism at nine microsatellite loci (284 individuals) was used to determine genetic variability and to identify the most probable panmictic units (using a Bayesian approach), after a significant departure from Hardy-Weinberg equilibrium was observed in the overall dataset (Western Amazon + Upper Madera). Bayesian analyses revealed at least three clusters in admixture in the five locations sampled in the Bolivian Amazon, whereas only two of these clusters were observed in the Western Amazon. Considering the migratory behaviour of B. rousseauxii, different life history strategies, including homing, are proposed to explain the cluster distribution. Our results are discussed in the light of the numerous threats to the species survival in the Madera basin, in particular dam and reservoir construction.

  20. Spontaneous behavior of basal Copionodontinae cave catfishes from Brazil (Teleostei, Siluriformes, Trichomycteridae

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    Bianca Rantin

    2015-08-01

    Full Text Available Cave animals are particularly interesting due to their behavioral specializations, resultant from evolution in isolation. We present data from a spontaneous behavior study (spatial distribution and preference for microhabitats of two troglobitic catfish from Brazil: Glaphyropoma spinosum and a new species of Copionodon. We compared the data with those obtained of a sympatric epigean species, Copionodon pecten. These Trichomycteridae species belong to a basal and apparently monophyletic subfamily – Copionodontinae, endemic to Chapada Diamantina, central Bahia state, eastern Brazil. We observed the fishes in natural and laboratory conditions through ad libitum and focal animal methods. Each spatial behavioral category (hidden, bottom, midwater, surface and wall swimming and stationary in the bottom was timed individually, with a sample of 12 specimens per species. Unlike most troglobitic fishes, cave copionodontines tested herein did not extend exploratory behavior to midwater, with benthonic and thigmotactic-related exploratory behavior. This behavior is possibly related to its feeding behavior specializations, strong territorialism and photophobic behavior. The epigean Copionodon species is also benthonic. The spatial behavior of the cave Copionodontinae could be interpreted as a retained and plesiomorphic character-state in relation to other trichomycterid catfishes.

  1. Anesthetic activity of Brazilian native plants in silver catfish (Rhamdia quelen

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    Lenise de Lima Silva

    Full Text Available There is an increasing demand for inexpensive and safe anesthetics that can reduce fish stress caused by some procedures such as capture and handling. In this context, the present study evaluated the potential of essential oils (EO of three Brazilian native plants (Hesperozygis ringens, Lippia sidoides and Ocotea acutifolia as anesthetics for the silver catfish - Rhamdia quelen. Moreover, an analysis was made of the chemical composition of these oils and their influence on stress parameter. EO of H. ringens and O. acutifolia were effective as anesthetics, without behavioral side effects. EO of O. acutifolia (150 µL L-1 promoted an increase in blood glucose level. Regarding to the composition, pulegone accounts for 96.63% of the EO of H. ringens, and caryophyllene oxide amounts to 56.90% of the EO of O. acutifolia. Two chemotypes, thymol and carvacrol (68.40% and 67.89%, respectively were verified for EO of L. sidoides. Both samples of EO of L. sidoides showed anesthetic activity in silver catfish, but exposure also caused loss of mucus and mortality. Thus, only the EO of H. ringens and O. acutifolia are advised for anesthetic use

  2. Effect injection of Ginger Extract on Development and Nucleus position of “Sangkuriang” Catfish Clarias sp. eggs

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    M. Zairin Junior

    2007-07-01

    Full Text Available One of methods could be applied to continuously meet the need of fish fry including catfish (Clarias sp. is artificial propagation by inducing ovulation and spawning.   As an alternative of existing method, in this study, ginger extract was intramuscularly injected to induce development of catfish eggs.  Ginger is known as an important regulator of the balance of arachidonat cycle.  The dose of ginger extract injected was 0, 0.5, 1.0 and 1.5 mL/kg broodstock.  The results of study showed that injection 100% of ginger extracts in all doses was insignificantly inducing development of egg diameter and its nucleus position of catfish.  Other chemicals exist in ginger extract might be functions as an obstacle for egg development of catfish. Keywords: catfish, Clarias sp., ginger, ovulation, egg   ABSTRAK Salah satu cara untuk memenuhi kebutuhan benih ikan termasuk lele (Clarias sp. secara kontinyu adalah penggunaan teknologi pembiakan buatan melalui perangsangan ovulasi dan pemijahan. Sebagai alternatif teknik yang sudah ada, dicobakan perangsangan perkembangan telur ikan lele menggunakan bahan ekstrak jahe yang dilakukan melalui penyuntikan secara intramuskular. Jahe telah dikenal sebagai suatu pengatur penting atas keseimbangan siklus arakidonat. Dosis ekstrak jahe yang disuntikkan adalah 0, 0,5, 1 dan 1,5 mL/kg induk. Penyuntikan 100% ekstrak jahe pada semua dosis perlakuan belum dapat merangsang perkembangan diameter dan posisi inti sel telur ikan lele sangkuriang. Adanya bahan lain yang terdapat pada jahe diduga sebagai penghambat bagi perkembangan telur ikan lele. Kata kunci: Ikan lele, Clarias sp., jahe, ovulasi,  sel telur

  3. Using 1-D 1H and 2-D 1H J-resolved NMR metabolomics to understand the effects of anemia in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  4. Using 1-D and 2-D J-resolved 1H-NMR metabolomics to understand the effects of anemia in channel catfish Ictalurus punctatus

    Science.gov (United States)

    Anemia is a widespread hematological disorder in vertebrates. In channel catfish (Ictalurus punctatus), anemia is a persistent problem in culture environments, however, its causes and physiological impacts are not well understood. To better understand the symptoms and characterize associated biomark...

  5. Diverse Early Life-History Strategies in Migratory Amazonian Catfish: Implications for Conservation and Management.

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    Jens C Hegg

    Full Text Available Animal migrations provide important ecological functions and can allow for increased biodiversity through habitat and niche diversification. However, aquatic migrations in general, and those of the world's largest fish in particular, are imperiled worldwide and are often poorly understood. Several species of large Amazonian catfish carry out some of the longest freshwater fish migrations in the world, travelling from the Amazon River estuary to the Andes foothills. These species are important apex predators in the main stem rivers of the Amazon Basin and make up the region's largest fishery. They are also the only species to utilize the entire Amazon Basin to complete their life cycle. Studies indicate both that the fisheries may be declining due to overfishing, and that the proposed and completed dams in their upstream range threaten spawning migrations. Despite this, surprisingly little is known about the details of these species' migrations, or their life history. Otolith microchemistry has been an effective method for quantifying and reconstructing fish migrations worldwide across multiple spatial scales and may provide a powerful tool to understand the movements of Amazonian migratory catfish. Our objective was to describe the migratory behaviors of the three most populous and commercially important migratory catfish species, Dourada (Brachyplatystoma rousseauxii, Piramutaba (Brachyplatystoma vaillantii, and Piraíba (Brachyplatystoma filamentosum. We collected fish from the mouth of the Amazon River and the Central Amazon and used strontium isotope signatures ((87Sr/(86Sr recorded in their otoliths to determine the location of early rearing and subsequent. Fish location was determined through discriminant function classification, using water chemistry data from the literature as a training set. Where water chemistry data was unavailable, we successfully in predicted (87Sr/(86Sr isotope values using a regression-based approach that related

  6. Diverse Early Life-History Strategies in Migratory Amazonian Catfish: Implications for Conservation and Management.

    Science.gov (United States)

    Hegg, Jens C; Giarrizzo, Tommaso; Kennedy, Brian P

    2015-01-01

    Animal migrations provide important ecological functions and can allow for increased biodiversity through habitat and niche diversification. However, aquatic migrations in general, and those of the world's largest fish in particular, are imperiled worldwide and are often poorly understood. Several species of large Amazonian catfish carry out some of the longest freshwater fish migrations in the world, travelling from the Amazon River estuary to the Andes foothills. These species are important apex predators in the main stem rivers of the Amazon Basin and make up the region's largest fishery. They are also the only species to utilize the entire Amazon Basin to complete their life cycle. Studies indicate both that the fisheries may be declining due to overfishing, and that the proposed and completed dams in their upstream range threaten spawning migrations. Despite this, surprisingly little is known about the details of these species' migrations, or their life history. Otolith microchemistry has been an effective method for quantifying and reconstructing fish migrations worldwide across multiple spatial scales and may provide a powerful tool to understand the movements of Amazonian migratory catfish. Our objective was to describe the migratory behaviors of the three most populous and commercially important migratory catfish species, Dourada (Brachyplatystoma rousseauxii), Piramutaba (Brachyplatystoma vaillantii), and Piraíba (Brachyplatystoma filamentosum). We collected fish from the mouth of the Amazon River and the Central Amazon and used strontium isotope signatures ((87)Sr/(86)Sr) recorded in their otoliths to determine the location of early rearing and subsequent. Fish location was determined through discriminant function classification, using water chemistry data from the literature as a training set. Where water chemistry data was unavailable, we successfully in predicted (87)Sr/(86)Sr isotope values using a regression-based approach that related the geology

  7. Evaluation of Local Feed Ingredients Based Diets on Growth Performance of African Catfish, Clarias gariepinus

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    Farahiyah, I. J.

    2016-12-01

    Full Text Available Formulating the right feed based on the requirements of the fish species is the main solver to reduce the high cost of aquaculture production. In formulating suitable diet, factors such as the sustainability or availability of the feed sources used, quality of the feeds and price of raw materials, need to be taken into account as well so that the diets can be formulated at least cost. Low cost diets using local feed ingredients were formulated in pelleted and extruded forms and fed to juvenile African catfish, Clarias gariepinus, to determine their effect on the growth performance of the fish. Two formulations were tested: Diet A - Formula 1 MARDI and Diet B - Formula 2 MARDI, which were formulated to be iso-caloric and iso-nitrogenous with 32 % digestible protein and energy value of 14 MJ/kg. A commercial diet (Diet C was included as a control. At 15 wk of culture, fish fed diet C showed the highest growth performance in terms of weight gain, feed conversion ratio (FCR and specific growth rate (SGR. There were no significant differences (P>0.05 found among diets for weight gain and feed intake. However, FCR and SGR of Diet C were significantly better (P<0.05 compared to the experimental diets. The best FCR was recorded in Diet C at 1.17, followed by Diet A (1.42 and Diet B (1.46. Diet C also had the highest SGR with 1.99%/day followed by Diet B (1.70 and Diet A (1.67. Cost of both diets, A and B, was RM 2.50/kg compared to RM 3.50/kg for Diet C. Although the FCR of the commercial feed was lower than the experimental diets, the production cost of fish was RM4.11/kg for the commercial feed compared to RM 3.54-3.65/kg for the experimental diets. It is concluded that local feed ingredients can be used in formulating diets for catfish and they have no detrimental effect on the growth of African catfish.

  8. Effect of nutrient restriction and re-feeding on calpain family genes in skeletal muscle of channel catfish (Ictalurus punctatus.

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    Elena Preziosa

    Full Text Available BACKGROUND: Calpains, a superfamily of intracellular calcium-dependent cysteine proteases, are involved in the cytoskeletal remodeling and wasting of skeletal muscle. Calpains are generated as inactive proenzymes which are activated by N-terminal autolysis induced by calcium-ions. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we characterized the full-length cDNA sequences of three calpain genes, clpn1, clpn2, and clpn3 in channel catfish, and assessed the effect of nutrient restriction and subsequent re-feeding on the expression of these genes in skeletal muscle. The clpn1 cDNA sequence encodes a protein of 704 amino acids, Clpn2 of 696 amino acids, and Clpn3 of 741 amino acids. Phylogenetic analysis of deduced amino acid sequences indicate that catfish Clpn1 and Clpn2 share a sequence similarity of 61%; catfish Clpn1 and Clpn3 of 48%, and Clpn2 and Clpn3 of only 45%. The domain structure architectures of all three calpain genes in channel catfish are similar to those of other vertebrates, further supported by strong bootstrap values during phylogenetic analyses. Starvation of channel catfish (average weight, 15-20 g for 35 days influenced the expression of clpn1 (2.3-fold decrease, P<0.05, clpn2 (1.3-fold increase, P<0.05, and clpn3 (13.0-fold decrease, P<0.05, whereas the subsequent refeeding did not change the expression of these genes as measured by quantitative real-time PCR analysis. Calpain catalytic activity in channel catfish skeletal muscle showed significant differences only during the starvation period, with a 1.2- and 1.4- fold increase (P<0.01 after 17 and 35 days of starvation, respectively. CONCLUSION/SIGNIFICANCE: We have assessed that fasting and refeeding may provide a suitable experimental model to provide us insight into the role of calpains during fish muscle atrophy and how they respond to changes in nutrient supply.

  9. Discovery of South American suckermouth armored catfishes (Loricariidae, Pterygoplichthys spp.) in the Santa Fe River drainage, Suwannee River basin, USA

    Science.gov (United States)

    Nico, Leo G.; Butt, Peter L.; Johnston, Gerald R.; Jelks, Howard L.; Kail, Matthew; Walsh, Stephen J.

    2012-01-01

    We report on the occurrence of South American suckermouth armored catfishes (Loricariidae) in the Suwannee River basin, southeastern USA. Over the past few years (2009-2012), loricariid catfishes have been observed at various sites in the Santa Fe River drainage, a major tributary of the Suwannee in the state of Florida. Similar to other introduced populations of Pterygoplichthys, there is high likelihood of hybridization. To date, we have captured nine specimens (270-585 mm, standard length) in the Santa Fe River drainage. One specimen taken from Poe Spring best agrees with Pterygoplichthys gibbiceps (Kner, 1854) or may be a hybrid with either P. pardalis or P. disjunctivus. The other specimens were taken from several sites in the drainage and include seven that best agree with Pterygoplichthys disjunctivus (Weber, 1991); and one a possible P. disjunctivus x P. pardalis hybrid. We observed additional individuals, either these or similar appearing loricariids, in Hornsby and Poe springs and at various sites upstream and downstream of the long (> 4 km) subterranean portion of the Santa Fe River. These specimens represent the first confirmed records of Pterygoplichthys in the Suwannee River basin. The P. gibbiceps specimen represents the first documented record of an adult or near adult of this species in open waters of North America. Pterygoplichthys disjunctivus or its hybrids (perhaps hybrid swarms) are already abundant and widespread in other parts of peninsular Florida, but the Santa Fe River represents a northern extension of the catfish in the state. Pterygoplichthys are still relatively uncommon in the Santa Fe drainage and successful reproduction not yet documented. However, in May 2012 we captured five adult catfish (two mature or maturing males and three gravid females) from a single riverine swallet pool. One male was stationed at a nest burrow (no eggs present). To survive the occasional harsh Florida winters, these South American catfish apparently use

  10. Histomorphological and mucin histochemical study of the alimentary canal of pangas catfish, Pangasius pangasius (Hamilton 1822

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    Javd Sadeghinezhad

    2017-06-01

    Full Text Available The present study describes the histological and mucin histochemical properties of the alimentary canal (AC of the pangas catfish, Pangasius pangasius. The results revealed that the mucosa of the oesophagus was lined by a stratified epithelium containing chloride cells and taste buds which suggested mechanic, gustatory and physiologic roles of the oesophagus in this species. The stomach mucosa was lined by a simple columnar epithelium. The lamina propria-submucosa in cardiac and fundic stomach contained gastric glands. The pyloric stomach had the thickest muscularis layer among all the parts of the AC. The villi showed the maximum height and width in the middle intestine. The tunica muscularis and serosa showed the thinnest thickness among all parts of AC. The mucin histochemistry showed that the goblet cells of oesophagus and intestine contained both neutral and acidic with carboxylated and sulfated mucins and there was not acidic mucins in epithelial cells of the stomach.

  11. Microglanis pataxo, a new catfish from southern Bahia coastal rivers, northeastern Brazil (Siluriformes: Pseudopimelodidae

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    Luisa M. Sarmento-Soares

    Full Text Available A new pseudopimelodid catfish of the genus Microglanis, collected in small rivers at the lower and middle Peruípe, middle Jucuruçu and Cahy basins, in the southeastern coast of the Bahia state, is described. The new species has a comparatively high number of anal fin proximal radials, 12, shaped as thin tubes, the last one bearing a laminar extension; a pectoral fin spine with a bony point and with a smaller number of serrations on its posterior border; seven pleural ribs and a narrow head width. We provide information on the external morphology and also on some osteological features for the new species. A key to species is presented for the coastal drainages between Rio de Janeiro and Bahia states.

  12. Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus

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    Qorie Astria

    2017-07-01

    Full Text Available ABSTRACT Outbreaks of infectious diseases due to Aeromonas hydrophila in catfish can cause high death rates (80–100%. Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem Musa cavendishii var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections A. hydrophila. The observed parameters were a total performance of production, hematological test, and water quality. Immersion of catfish seeds with stem juice was performed at a concentration of 5 mL/L, 13 mL/L, and 21 mL/L for 30 minutes. Each treatment consisted of three replications. A  total of 15 fishes were immersed in 1.5 L water. At day-9 after immersion, fish was infected by A. hydrophila bacteria at a dose of 104 cfu/mL. The results showed that fish treated with banana stem juice at a concentration of 13 mL/L had the survival rate of 53.33±6.67% which is higher than positive control (33.33±6.67%. Based on hematology observations on the 3rd day post-challenge test (H12 with A. hydrophila, total erythrocytes, hemoglobin, total leukocytes, phagocytic activity, differential leukocyte, and lysozyme activity was highest in treatment13 mL/L. Lower feed conversion ratio (1.08±0.04 were also obtained in treatment 13 mL/L. Thus Immersion of seeds in stem juice can boost the immune system against infections A. hydrophila. Keywords: Aeromonas hydrophila, ambon banana stem juice, soaking, catfish, immune system  ABSTRAK Wabah penyakit akibat infeksi Aeromonas hydrophila pada ikan lele dapat menyebabkan tingkat kematian yang tinggi (80–100%. Penanggulangan penyakit ikan dapat dilakukan menggunakan fitofarmaka untuk mencegah ataupun mengobati penyakit ikan. Salah satu fitofarmaka yang dapat digunakan dalam upaya pencegahan penyakit ikan adalah batang

  13. Harttia merevari, a new species of catfish (Siluriformes: Loricariidae from Venezuela

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    Francisco Provenzano R.

    Full Text Available Harttia merevari, a new species of loricariid catfish, is described from eight specimens captured in the upper Caura River, Orinoco River basin, Venezuela. The new species is recognized by the following combination of characters: abdomen naked; two or three preanal plates; a bony plate before each branchial opening; seven lateral plates between the pectoral and pelvic fins; maxillary barbel short and attached to the oral disk by a fleshy fold; head dorsal surface and anterior portion of the body light or dark yellow with numerous, round black spots; posterior region of the body light or dark yellow with five black transverse bands, dorsal central area of the two anterior bands diffused. The discovery of this new species extends the distribution of the genus northwest to include the Orinoco River basin on the northern slope of the Guyana shield.

  14. De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

    Science.gov (United States)

    Schulze, Thomas T.; Ali, Jonathan M.; Bartlett, Maggie L.; McFarland, Madalyn M.; Clement, Emalie J.; Won, Harim I.; Sanford, Austin G.; Monzingo, Elyssa B.; Martens, Matthew C.; Hemsley, Ryan M.; Kumar, Sidharta; Gouin, Nicolas; Kolok, Alan S.; Davis, Paul H.

    2016-01-01

    Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina® platform and processed using PRINSEQ. The transcriptome assembly was performed using TRINITY assembler. Transcriptome validation was performed by functional characterization with KOG, KEGG, and GO analyses. Additionally, differential expression analysis highlights sex-specific expression patterns, and a list of endocrine and oxidative stress related transcripts are included. PMID:27672404

  15. Henneguya exilis Kudo associated with granulomatous branchitis of channel catfish Ictalurus punctatus (Rafinesque).

    Science.gov (United States)

    Duhamel, G E; Kent, M L; Dybdal, N O; Hedrick, R P

    1986-07-01

    Outbreaks of a chronic branchitis in channel catfish Ictalurus punctatus (Rafinesque) were observed on four fish farms throughout the state of California from November 1982 to April 1984. Severe granulomatous inflammation of the gill filaments with necrosis of the cartilage of the gill ray and diffuse epithelial hyperplasia, resulting in extensive fusion of gill lamellae, was present on histologic examination of gill specimens from 35 out of 44 fish examined. Numerous, small trophozoites morphologically consistent with presporogonic myxosporean parasites were consistently associated with the inflammatory process. Mature spores of Henneguya exilis Kudo were present in large numbers in gill specimens from two fish and only occasionally in 22 others. Similar cases referred to as "Hamburger Gill Disease" or "proliferative gill disease" have been known to occur in the south-central United States. This report describes the morphologic changes of this condition and discusses its possible pathogenesis.

  16. Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue

    Science.gov (United States)

    Meinertz, J.R.; Dawson, V.K.; Gingerich, W.H.; Cheng, B.; Tubergen, M.M.

    1994-01-01

    A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues i n channel catfish (ictalurus punctatus) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile=water (1 + 1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 Mum filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile-methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), Centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a c18 column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 To 104%, and relative standard deviations ranged from 1.06 To 5.58% In samples spiked at concentrations of 10.0-863.8 Ng/g. The method detection limit for sarafloxacin was 1.4 Ng/g.

  17. Exposure to sublethal concentrations of copper changes biochemistry parameters in silver catfish, Rhamdia quelen, Quoy & Gaimard.

    Science.gov (United States)

    Pretto, Alexandra; Loro, Vania Lucia; Silva, Vera M Machado; Salbego, Joseânia; de Menezes, Charlene Cavalheiro; Souza, Carine de Freitas; Gioda, Carolina Rosa; Baldisserotto, Bernardo

    2014-04-01

    The effects of Cu exposure on catalase (CAT) and acetylcholinesterase (AChE) activity, formation of thiobarbituric acid-reactive species (TBARS) and metabolic parameters were evaluated in silver catfish (Rhamdia quelen). The fish were exposed for 45 days to 0, 16 and 29 μg/L Cu. The fish that were exposed to Cu exhibited lower TBARS levels in the muscle and higher TBARS levels in the liver. They also showed lower CAT activity in the liver and lower AChE activity in the brain and muscle. Higher glucose and lactate and lower protein plasma levels were observed in the fish exposed to Cu. The changes in the hepatic metabolic parameters were Cu concentration dependent. In the muscle, lower glycogen and higher lactate levels were observed in the fish exposed to Cu. Alterations in the metabolic parameters showed a preference for the anaerobic pathway of energy production and liver protein catabolism to supply the energy demand.

  18. Invasion of South American suckermouth armoured catfishes Pterygoplichthys spp. (Loricariidae in Kerala, India - a case study

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    A. Bijukumar

    2015-03-01

    Full Text Available This paper documents the occurrence of the exotic South American suckermouth armoured catfishes (Loricariidae of the genus Pterygoplichthys spp. in the drainages of Thiruvananthapuram City, Kerala.  The morphological taxonomy revealed that the specimens are closely related to Pterygoplichthys disjunctivus (Weber, 1991 and P. pardalis (Castelnau, 1855, in addition to intermediary forms of unknown identity.  DNA barcoding using the mitochondrial DNA cytochrome c oxidase 1 (CO1 also failed to establish the identity of the invaded species.  There may be possible hybridisation in aquariums or in fish farms or in the wild, but this needs to be found out aided by detailed studies incorporating different molecular markers and with sequences of topotypes.  The possible threats due to Pterygoplichthys spp. invasion and management options are discussed in the paper. 

  19. Catalysis of Silver catfish Major Hepatic Glutathione Transferase proceeds via rapid equilibrium sequential random Mechanism

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    Ayodele O. Kolawole

    Full Text Available Fish hepatic glutathione transferases are connected with the elimination of intracellular pollutants and detoxification of organic micro-pollutants in their aquatic ecosystem. The two-substrate steady state kinetic mechanism of Silver catfish (Synodontis eupterus major hepatic glutathione transferases purified to apparent homogeneity was explored. The enzyme was dimeric enzyme with a monomeric size of 25.6 kDa. Initial-velocity studies and Product inhibition patterns by methyl glutathione and chloride with respect to GSH-CDNB; GSH-ρ-nitrophenylacetate; and GSH-Ethacrynic acid all conforms to a rapid equilibrium sequential random Bi Bi kinetic mechanism rather than steady state sequential random Bi Bi kinetic. α was 2.96 ± 0.35 for the model. The pH profile of Vmax/KM (with saturating 1-chloro-2,4-dinitrobenzene and variable GSH concentrations showed apparent pKa value of 6.88 and 9.86. Inhibition studies as a function of inhibitor concentration show that the enzyme is a homodimer and near neutral GST. The enzyme poorly conjugates 4-hydroxylnonenal and cumene hydroperoxide and may not be involved in oxidative stress protection. The seGST is unique and overwhelmingly shows characteristics similar to those of homodimeric class Pi GSTs, as was indicated by its kinetic mechanism, substrate specificity and inhibition studies. The rate- limiting step, probably the product release, of the reaction is viscosity-dependent and is consequential if macro-viscosogen or micro-viscosogen. Keywords: Silver catfish, Glutathione transferase, Steady-state, Kinetic mechanism, Inhibition

  20. Pentachlorophenol-Induced Cytotoxic, Mitogenic, and Endocrine-Disrupting Activities in Channel Catfish, Ictalurus punctatus

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    Paul B. Tchounwou

    2004-09-01

    Full Text Available Pentachlorophenol (PCP is an organochlorine compound that has been widely used as a biocide in several industrial, agricultural, and domestic applications. Although it has been shown to induce systemic toxicity and carcinogenesis in several experimental studies, the literature is scarce regarding its toxic mechanisms of action at the cellular and molecular levels. Recent investigations in our laboratory have shown that PCP induces cytotoxicity and transcriptionally activates stress genes in human liver carcinoma (HepG2 cells [1]. In this research, we hypothesize that environmental exposure to PCP may trigger cytotoxic, mitogenic, and endocrine-disrupting activities in aquatic organisms including fish. To test this hypothesis, we carried out in vitro cultures of male channel catfish hepatocytes, and performed the fluorescein diacetate assay (FDA to assess for cell viability, and the Western Blot analysis to assess for vitellogenin expression following exposure to PCP. Data obtained from FDA experiments indicated a strong dose-response relationship with respect to PCP cytotoxicity. Upon 48 hrs of exposure, the chemical dose required to cause 50% reduction in cell viability (LD50 was computed to be 1,987.0 + 9.6 μg PCP/mL. The NOAEL and LOAEL were 62.5 + 10.3 μg PCP/mL and 125.0+15.2 μg PCP/mL, respectively. At lower levels of exposure, PCP was found to be mitogenic, showing a strong dose- and time-dependent response with regard to cell proliferation. Western Blot analysis demonstrated the potential of PCP to cause endocrine-disrupting activity, as evidenced by the up regulation of the 125-kDa vitellogenin protein the hepatocytes of male channel catfish.

  1. Temperature dependence of cardiac sarcoplasmic reticulum and sarcolemma in the ventricle of catfish (Clarias gariepinus

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    El-Sabry Abu-Amra

    2015-10-01

    The sarcolemmal Ca2+ contribution of activator Ca2+ was greater at a test temperature of 30 °C as assessed by verapamil. Whereas the SR-Ca2+ contribution was higher at 20 and 30 °C and a frequency rate of 0.2 and 0.4 Hz as assessed by caffeine and adrenaline, respectively. Bradykinin potentiating factor (BPF7 which was isolated from jelly fish (Cassiopea andromeda decreased the cardiac force developed at a frequency rate of 0.2 Hz and a temperature of 20 °C, whereas it increased the force developed at frequency rates of 0.2 and 0.4 Hz at 30 °C. These results indicate that BPF7 may act like verapamil in reducing the cardiac force through blocking the sarcolemmal Ca2+ channels at low temperature and like adrenaline in an increase of the cardiac force developed at warm temperature and the high frequency rate through stimulation of SR-Ca2+ activator. Therefore, this study indicates that the sarcolemmal Ca2+ influx and the SR-Ca2+ release contributors of activator Ca2+ for cardiac force development in the catfish heart were significantly greater at warm temperature and at the pacing frequency rates of 0.2 and 0.4 Hz as assessed by verapamil, adrenaline, caffeine and BPF7. However, the relative contribution of the sarcolemmal Ca2+ influx in the development of cardiac force in the catfish heart was greater than that of SR-Ca2+ release.

  2. Growth performance of African catfish (Clarias sp. juvenile fed on the diets containing various chromium content

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    H. Aryansyah

    2007-07-01

    Full Text Available This experiment was conducted to determine the effect of different dietary chromium level on the growth performance of catfish (Clarias sp. juvenile. Five experimental diets contain different chromium level, namely diet A (as a control diet 0.01; B 1.30; C 2.60; D 3.90 and E 5.20 mg/kg diet were used in this experiment. Fish with body weight of  5.57 ± 0.01 g/ind, fed on the experimental diet, twice a day, at satiation. Feeding trial was conducted for 60 days. Based on the evaluation of protein level of the whole body, protein and lipid retention, daily growth rate and feed efficiency, it was concluded that the optimum dietary chromium level for catfish  juvenile was 2.60 mg/kg diet. Keywords: chromium, growth, Clarias sp.   ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh pemberian kromium dengan level berbeda terhadap pertumbuhan ikan lele (Clarias sp. juvenil.  Lima jenis pakan yang mengandung kromium berbeda, yaitu pakan A  (kontrol 0,01; B 1,30; C 2,60; D 3,90 dan pakan E 5,20 mg/kg digunakan dalam penelitian ini. Ikan dengan bobot 5,57 ± 0,01 g/ind., diberi pakan 2 kali sehari secara satiasi.  Pemeliharaan ikan dilakukan selama 60 hari.  Berdasarkan analisa retensi protein, kandungan protein dan retensi lemak dalam tubuh ikan lele, disimpulkan bahwa kadar kromium yang optimum untuk juvenil ikan lele adalah 2,60 mg/kg pakan. Kata kunci: kromium, pertumbuhan, Clarias sp.

  3. Effects of strains on growth performances of triploid bighead catfish, Clarias macrocephalus Günther, 1864

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    Satid Chatchaiphan

    2016-07-01

    Full Text Available Despite its importance, limited empirical information is available on the effects of parental strains on the performance of triploid aquatic animals, especially of tropical species. The present study was conducted to evaluate the impacts of strains on the growth performance of the triploid bighead catfish (Clarias macrocephalus Günther, 1864 which was produced using cold shock (7 °C, 25 min shock duration, applied to newly fertilized eggs obtained from all possible crosses between two catfish strains. The fingerlings of each group were reared until they were age 240 d. At age 60 d, the triploid fish had a lower specific growth rate (SGR, absolute growth rate (AGR, body length and body weight than the diploid counterparts. However, at harvest (age 240 d body length and body weight were not different between the two groups while SGR of the cold shock group was higher than the diploid during age 121–240 d. The results revealed that parental strains did not show significant direct effects on the growth performance of triploids, but the interaction between paternal strain × shock was significant for the following traits: body length at age 90 d, body weight at age 90 d and 120 d, AGR for age 61–90 d, and SGR for age 61–90 d and 91–120 d. The interaction between paternal × maternal strain × shock was significant for body length, body weight at age 180 d and AGR for age 121–180 d. These results suggested that the growth performance of triploid C. macrocephalus cannot be enhanced by improving the parental strains; rather, improvement may be accomplished by selecting the parental strains based on triploid performance.

  4. Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch).

    Science.gov (United States)

    Marimuthu, Kasi; Muthu, Narmataa; Xavier, Rathinam; Arockiaraj, Jesu; Rahman, M Aminur; Subramaniam, Sreeramanan

    2013-01-01

    Buprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus) as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0.05, 0.5, 5, 25, 50 and 100 mg/L) of buprofezin. Each concentration was assessed in five replicates. Eggs were artificially fertilized and 200 eggs and larvae were subjected to a static bath treatment for all the concentrations. The mortality of embryos was significantly increased with increasing buprofezin concentrations from 5 to 100 mg/L (pbuprofezin acute toxicity tests were evaluated using probit analysis. The 24 h LC50 value (with 95% confidence limits) of buprofezin for embryos was estimated to be 6.725 (3.167-15.017) mg/L. The hatching of fish embryos was recorded as 68.8, 68.9, 66.9, 66.4, 26.9, 25.1 and 0.12% in response to 7 different concentrations of buprofezin, respectively. The mortality rate of larvae significantly (pbuprofezin concentrations exposed to 24-48 h. The 24 and 48 h LC50 values (with 95% confidence limits) of buprofezin for the larvae was estimated to be 5.702 (3.198-8.898) and 4.642 (3.264-6.287) mg/L respectively. There were no significant differences (p>0.05) in the LC50 values obtained at 24 and 48 h exposure times. Malformations were observed when the embryos and larvae exposed to more than 5 mg/L. The results emerged from the study suggest that even the low concentration (5 mg/L) of buprofezin in the aquatic environment may have adverse effect on the early embryonic and larval development of African catfish.

  5. Molecular systematic and historical biogeography of the armored Neotropical catfishes Hypoptopomatinae and Neoplecostominae (Siluriformes: Loricariidae).

    Science.gov (United States)

    Chiachio, Márcio Cesar; Oliveira, Claudio; Montoya-Burgos, Juan I

    2008-11-01

    The Neotropics possess the greatest freshwater fish diversity of the world, rendering the study of their evolutionary history extremely challenging. Loricariidae catfishes are one of the most diverse components of the Neotropical ichthyofauna and despite a long history of classification, major issues still need elucidation. Based on a nuclear gene, we present a robust phylogeny of two former loricariid subfamilies: Hypoptopomatinae and Neoplecostominae. Our results show that Neoplecostominae is nested within Hypoptopomatinae, and is the sister group to the former Otothyrini tribe. According to our results, supplemented by morphological observations, we erect two new subfamilies, the Otothyrinae and a new Hypoptopomatinae, and modify the Neoplecostominae by including the genus Pseudotocinclus. The uncovered evolutionary relationships allow a detailed analysis of their historical biogeography. We tested two Dispersal-Extinction-Cladogenesis models for inferring the distribution range evolution of the new subfamilies, and show that the model having no constrains performs better than a model constraining long-range dispersal. The Maximum Likelihood reconstructions of ancestral ranges showed a marked division between the Amazonian origin of the Hypoptopomatinae and the eastern coastal Brazil+Upper Paraná origin of the Neoplecostominae and Otothyrinae. Markedly few instances of dispersal across the border separating the Amazon basin and the Paraná-Paraguay+eastern coastal Brazil+Uruguay were reconstructed. This result is in clear contrast with the historical biogeography of many Neotropical fishes, including other Loricariidae. Part of the dispersal limitation may be explained by divergent ecological specialization: lowland rivers versus mountain streams habitats. Moreover, because most species of the new subfamilies are small, we hypothesize that body size-related effects might limit their dispersal, like predation and energetic cost to migration. Finally

  6. Biochemistry, cytogenetics and bioaccumulation in silver catfish (Rhamdia quelen) exposed to different thorium concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Lizelia M; Kochhann, Daiani; Becker, Alexssandro G; Pavanato, Maria A [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Llesuy, Susana F [Departamento de Quimica Analitica y Fisicoquimica, Universidad de Buenos Aires, Buenos Aires (Argentina); Loro, Vania L; Raabe, Alice [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Mesko, Marcia F [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Polo em Cruz Alta, Universidade Estadual do Rio Grande do Sul, 98025-810 Cruz Alta, RS (Brazil); Flores, Erico M.M.; Dressler, Valderi L [Departamento de Quimica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil); Baldisserotto, Bernardo [Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS (Brazil)

    2008-07-30

    The objective of this study was to evaluate the effect of thorium (Th) bioaccumulation on the metabolism of silver catfish (Rhamdia quelen) through biochemical parameters of the muscle (glycogen, glucose, lactate, protein, and ammonia). In addition, lipidic peroxidation levels (TBARS), catalase (CAT) and glutathione-S-transferase (GST) in the gills and in hepatic and muscular tissues were also analyzed. Cytogenetic parameters were studied through the evaluation of nuclear abnormalities in red blood cells. Silver catfish juveniles were exposed to different waterborne Th levels (in {mu}g L{sup -1}): 0 (control), 25.3 {+-} 3.2, 69.2 {+-} 2.73, 209.5 {+-} 17.6, and 608.7 {+-} 61.1 for 15 days. The organs that accumulated the highest Th levels were the gills and skin. The increase of waterborne Th concentration corresponded to a progressive increase of Th levels in the gills, liver, skin and kidneys, with the highest accumulation in the gills and skin. Metabolic intermediates in the muscle were altered by Th exposure, but no clear relationship was found. CAT and GST activities in the hepatic and muscular tissues of this species suggest that the enzymatic activities can be stimulated at the lowest Th levels and inhibited at the higher levels (mainly in 608.7 {mu}g L{sup -1}). The results of the cytogenetic assay contribute to this hypothesis because the higher toxicity in blood samples was found in juveniles exposed to 69.2 and 209.5 {mu}g L{sup -1} Th.

  7. Distribution, Innervation, and Cellular Organization of Taste Buds in the Sea Catfish, Plotosus japonicus.

    Science.gov (United States)

    Nakamura, Tatsufumi; Matsuyama, Naoki; Kirino, Masato; Kasai, Masanori; Kiyohara, Sadao; Ikenaga, Takanori

    2017-01-01

    The gustatory system of the sea catfish Plotosus japonicus, like that of other catfishes, is highly developed. To clarify the details of the morphology of the peripheral gustatory system of Plotosus, we used whole-mount immunohistochemistry to investigate the distribution and innervation of the taste buds within multiple organs including the barbels, oropharyngeal cavity, fins (pectoral, dorsal, and caudal), and trunk. Labeled taste buds could be observed in all the organs examined. The density of the taste buds was higher along the leading edges of the barbels and fins; this likely increases the chance of detecting food. In all the fins, the taste buds were distributed in linear arrays parallel to the fin rays. Labeling of nerve fibers by anti-acetylated tubulin antibody showed that the taste buds within each sensory field are innervated in different ways. In the barbels, large nerve bundles run along the length of the organ, with fascicles branching off to innervate polygonally organized groups of taste buds. In the fins, nerve bundles run along the axis of fin rays to innervate taste buds lying in a line. In each case, small fascicles of fibers branch from large bundles and terminate within the basal portions of the taste buds. Serotonin immunohistochemistry demonstrated that most of the taste buds in all the organs examined contained disk-shaped serotonin-immunopositive cells in their basal region. This indicates a similar organization of the taste buds, in terms of the existence of serotonin-immunopositive basal cells, across the different sensory fields in this species. © 2017 S. Karger AG, Basel.

  8. Sound production and pectoral spine locking in a Neotropical catfish (Iheringichthys labrosus, Pimelodidae

    Directory of Open Access Journals (Sweden)

    Javier S. Tellechea

    2011-01-01

    Full Text Available Catfishes may have two sonic organs: pectoral spines for stridulation and swimbladder drumming muscles. The aim of this study was to characterize the sound production of the catfish Iheringichthys labrosus. The I. labrosus male and female emits two different types of sounds: stridulatory sounds (655.8 + 230 Hz consisting of a train of pulses, and drumming sounds (220 + 46 Hz, which are composed of single-pulse harmonic signals. Stridulatory sounds are emitted during abduction of the pectoral spine. At the base of the spine there is a dorsal process that bears a series of ridges on its latero-ventral surface, and by pressing the ridges against the groove (with an unspecialized rough surface during a fin sweep, the animal produce a series of short pulses. Drumming sound is produced by an extrinsic sonic muscle, originated on a flat tendon of the transverse process of the fourth vertebra and inserted on the rostral and ventral surface of the swimbladder. The sounds emitted by both mechanisms are emitted in distress situation. Distress was induced by manipulating fish in a laboratory tank while sounds were recorded. Our results indicate that the catfish initially emits a stridulatory sound, which is followed by a drumming sound. Simultaneous production of stridulatory and drumming sounds was also observed. The catfish drumming sounds were lower in dominant frequency than stridulatory sounds, and also exhibited a small degree of dominant frequency modulation. Another behaviour observed in this catfish was the pectoral spine locking. This reaction was always observed before the distress sound production. Like other authors outline, our results suggest that in the catfish I. labrosus stridulatory and drumming sounds may function primarily as a distress call.Bagres podem apresentar dois órgãos sonoros: o espinho peitoral para o som peitoral ou estridulatório e o músculo sonoro da bexiga natatória. O objetivo deste trabalho foi caracterizar a produ

  9. Isolation and initial characterisation of complement components C3 and C4 of the nurse shark and the channel catfish.

    Science.gov (United States)

    Dodds, A W; Smith, S L; Levine, R P; Willis, A C

    1998-01-01

    Complement components C3 and C4 have been isolated from the serum of the nurse shark (Ginglymostoma cirratum) and of the channel catfish (Ictalurus punctatus). As in the higher vertebrates, the fish C4 proteins have three-chain structures while the C3 proteins have two-chain structures. All four proteins have intra-chain thioesters located within their highest molecular mass polypeptides. N-terminal sequence analysis of the polypeptides has confirmed the identity of the proteins. In all cases except the catfish C3 alpha-chain, which appears to have a blocked N-terminus, sequence similarities are apparent in comparisons with the chains of C3 and C4 from higher vertebrates. We have confirmed that the activity/protein previously designated C2n is the nurse shark analogue of mammalian C4. This is the first report of structural evidence for C4 in both the bony and cartilaginous fish.

  10. Persistent organic pollutants and histological lesions in Mayan catfish Ariopsis assimilis from the Bay of Chetumal, Mexico.

    Science.gov (United States)

    Noreña-Barroso, E; Simá-Alvarez, R; Gold-Bouchot, G; Zapata-Pérez, O

    2004-02-01

    Livers of catfish (Ariopsis assimilis) from the Bay of Chetumal were analyzed for organochlorine compounds and hydrocarbons as part of a study to diagnose the environmental health of the Bay after a catfish mass mortality that occurred in 1996. The presence of histological lesions in several organs of the fish as result of chemical exposure was also evaluated. The concentrations of organic pollutants found in the Bay may be considered high if compared to the levels reported for sites affected by chemical pollution. High prevalences of cellular alteration histopathologies were found in liver, including hepatic tumors. The presence of some lesions may be related statistically to environmental pollution in the Bay, specially with chlorinated compounds.

  11. Changes in brain monoamine levels and monoamine oxidase activity in the catfish, Clarias batrachus, during chronic treatments with mercurials

    International Nuclear Information System (INIS)

    Kirubagaran, R.; Joy, K.P.

    1990-01-01

    In mammals, the central nervous system is the primary target for CH 3 Hg poisoning which is clinically known as Minamata disease. Hg is a widely recognized neurotoxin and has been reported to impair brain monoamine neurotransmitter metabolism. Reports on effects of Hg on brain monoamine activity in fishes are scarce. In the present study, therefore, changes in the brain monoamine levels and the degradation enzyme, monoamine oxidase (MAO), are described in the catfish, Clarias batrachus, exposed to sublethal concentrations of mercuric chloride (HgCl 2 -inorganic Hg), methylmercuric chloride (CH 3 HgCl-organic Hg), and a commercial mercurial fungicide formulation, emisan 6 (methoxyethyl Hg-organic Hg) for 45, 90 and 180 d during gonadal recrudescence. These intervals correspond to late preparatory, prespawning and spawning phases, respectively, of the annual reproductive cycle of the catfish

  12. Discovering the African freshwater "torpedo": legendary Ethiopia, religious controversies, and a catfish capable of reanimating dead fish.

    Science.gov (United States)

    Piccolino, Marco; Finger, Stanley; Barbara, Jean-Gaël

    2011-07-01

    The electric catfishes of African rivers and lakes, once depicted on Egyptian tomb art, have been largely overlooked in histories and reviews of electric fish biology and animal electricity. This article examines how Westerners, especially Dominican and Jesuit missionaries, discovered them in Ethiopia and other parts of Africa at the beginning of the seventeenth century. What transpired took place against the backdrop of tales involving the Bible, Prester John's mythical empire, and imaginary animals with fabulous powers. In effect, how they were found is related to attempts to convert Ethiopian Christians to true Catholicism, hopes of discovering great riches, and opportunities to trade, and not with the efforts of skilled natural philosophers to document and conduct experiments on the wildlife of this continent. Nevertheless, the early descriptions by Europeans circulated, and during the next century these catfishes began to be used in experiments that helped to make animal electricity a reality.

  13. Henneguya laterocapsulata Landsberg, 1987 (Myxosporea, Myxozoa) in cultured hybrid African catfish: Ultrastructure of the parasite-host interface.

    Science.gov (United States)

    Obiekezie, A; Schmahl, G

    1993-02-19

    The ultrastructure of the host-parasite interface was studied in Henneguya laterocapsulata, parasitizing the skin of hybrid catfishes (Clarias gariepinus × Heterobranchus bidorsalis) in Nigeria. The plasmodia were located between malpighian cells, which are the main elements of the multilayered fish epidermis, and were bordered by a single cell membrane. The desmosomal junctions between the malpighian cells were forced apart by finger-like protrusions of the Plasmodium. These plasmodial protrusions finally ran into the host cell without disrupting of the host cell membrane and formed network-like extensions. At the margin of the plasmodium an extensive vacuolization occurred, leading to a wavy surface. Infections with H. laterocapsulata may be an adverse factor in the large-scale production of hybrid catfish fingerlings used for aquaculture in Africa. Copyright © 1993 Gustav Fischer Verlag · Stuttgart · Jena · New York. Published by Elsevier GmbH.. All rights reserved.

  14. First record of the Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787 from Turkish marine waters

    Directory of Open Access Journals (Sweden)

    Servet Ahmet Doğdu

    2016-05-01

    Full Text Available Three specimens of striped eel catfish, Plotosus lineatus (Thunberg, 1787 were caught by a spear gun at depths of 20 m on 4 April 2016 from Iskenderun Bay (Çevlik harbour, Northeastern Mediterranean coast of Turkey. With the present study, P. lineatus is the first time reported from Turkish marine waters, in the Iskenderun Bay, the Northeastern Mediterranean and also this is the first Plotosid species recorded from Turkish Seas.

  15. The Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787, (Osteichtyes: Siluriformes, a new record from the Mediterranean

    Directory of Open Access Journals (Sweden)

    Daniel Golani

    2002-09-01

    Full Text Available The striped eel catfish, Plotosus lineatus (Thunberg, 1787, is recorded for the first time from the eastern Mediterranean coast of Israel. Seventeen specimens of this highly-venomous fish were caught by a commercial trawler at depths of 20 m. This species´ occurrence in the Mediterranean is the result of migration from the Red Sea via the Suez Canal (Lessepsian migration.

  16. Gelatin hydrolysates from farmed Giant catfish skin using alkaline proteases and its antioxidative function of simulated gastro-intestinal digestion.

    Science.gov (United States)

    Ketnawa, Sunantha; Martínez-Alvarez, Oscar; Benjakul, Soottawat; Rawdkuen, Saroat

    2016-02-01

    This work aims to evaluate the ability of different alkaline proteases to prepare active gelatin hydrolysates. Fish skin gelatin was hydrolysed by visceral alkaline-proteases from Giant catfish, commercial trypsin, and Izyme AL®. All antioxidant activity indices of the hydrolysates increased with increasing degree of hydrolysis (Pfish skin, could serve as a potential source of functional food ingredients for health promotion. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Sex-biased miRNAs in gonad and their potential roles for testis development in yellow catfish.

    Science.gov (United States)

    Jing, Jing; Wu, Junjie; Liu, Wei; Xiong, Shuting; Ma, Wenge; Zhang, Jin; Wang, Weimin; Gui, Jian-Fang; Mei, Jie

    2014-01-01

    Recently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. We observed relative lower expression of several miR-200 family members, including miR-141 and miR-429 in YY testis compared with XY testis. Histological analysis indicated a higher degree of testis maturity in YY super-males compared with XY males, as shown by larger spermatogenic cyst, more spermatids and fewer spermatocytes in the spermatogenic cyst. Moreover, five miR-200 family members were significantly up-regulated in testis when treated by 17α-ethinylestradiol (EE2), high dose of which will impair testis development and cell proliferation. The down-regulation of miR-141 and 429 coincides with the progression of testis development in both yellow catfish and human. At last, the expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs, some of that are potentially involved in testis development and spermatogenesis.

  18. Expression profiling of c-kit and its impact after esiRNA silencing during gonadal development in catfish.

    Science.gov (United States)

    Laldinsangi, C; Senthilkumaran, B

    2018-04-03

    C-kit receptor is a member of a family of growth factor receptors that have tyrosine kinase activity, and are involved in the transduction of growth regulatory signals across plasma membrane by activation of its ligand, kitl/scf. The present study analysed mRNA and protein expression profiles of c-kit in the gonads of catfish, Clarias gariepinus, using real time PCR, in situ hybridization and immunohistochemistry. Tissue distribution analysis revealed higher expression mainly in the catfish gonads. Ontogeny studies showed minimal expression during early developmental stages and highest during 50-75 days post hatch, and the dimorphic expression in gonads decreased gradually till adulthood, which might suggest an important role for this gene around later stages of sex differentiation and gonadal development. Expression of C-kit was analysed at various phases of gonadal cycle in both male and female, which showed minimal expression during the resting phase, and higher expression in male compared to females during the pre-spawning phase. In vitro and in vivo induction using human chorionic gonadotropin elevated the expression of c-kit indicating the regulatory influence of hypothalamo-hypophyseal axis. In vivo transient gene silencing using c-kit-esiRNA in adult catfish during gonadal recrudescence showed a decrease in c-kit expression, which affected the expression level of germ cell meiotic marker sycp3, as well as several factors and steroidogenic enzyme genes involved in germ cell development. Decrease in the levels of serum 11-KT and T were also observed after esiRNA silencing. The findings of this study suggest that c-kit has an important role in the process of germ cell proliferation, development and maturation during gonadal development and recrudescence in catfish. Copyright © 2018. Published by Elsevier Inc.

  19. Stable carbon and nitrogen incorporation in blood and fin tissue of the catfish Pterygoplichthys disjunctivus (Siluriformes, Loricariidae)

    OpenAIRE

    German, Donovan P.; Miles, Richard D.

    2010-01-01

    A feeding trial was performed in the laboratory with the catfish species Pterygoplichthys disjunctivus to determine stable carbon (13C) and nitrogen (15 N) turnover rates and discrimination factors in non-lethally sampled tissues (red blood cells, plasma solutes, and fin). A second feeding trial was conducted to determine what P. disjunctivus could assimilate from low-quality wood-detritus—refractory polysaccharides (e.g., cellulose), or soluble wood-degradation products inherent in wood-detr...

  20. CHARACTERIZATION OF THE RECEPTOR FOR GONADOTROPIN-RELEASING HORMONE IN THE PITUITARY OF THE AFRICAN CATFISH, CLARIAS-GARIEPINUS

    NARCIS (Netherlands)

    de Leeuw, R.; Conn, P. M.; van't Veer, C.; Goos, H. J.; van Oordt, P. G.

    1988-01-01

    Receptors for gonadotropin-releasing hormone (GnRH) were characterized using a radioligand prepared from a superactive analog of salmon GnRH (sGnRH), D-Arg(6)-Pro(9)-sGnRH-NEt (sGnRHa). Binding of(125)I-sGnRHa to catfish pituitary membrane fractions reached equilibrium after 2 h incubation at 4°C.

  1. Redescription of Proteocephalus glanduligerus (Cestoda: Proteocephalidea), a parasite of clariid catfishes in Africa with a unique glandular apical organ

    Czech Academy of Sciences Publication Activity Database

    Scholz, Tomáš; de Chambrier, A.; Beletew, M.; Mahmoud, Z. N.

    2009-01-01

    Roč. 95, č. 2 (2009), s. 443-449 ISSN 0022-3395 R&D Projects: GA ČR GA524/04/0342; GA ČR GA524/08/0885; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Proteocephalidae * Africa * catfishes * apical organ * morphology * redescription Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.195, year: 2009

  2. Determination of Lactic Acid Bacteria Viability in the Small Intestine of Catfish (Pangasius djambal by Using the 32P Radioisotope

    Directory of Open Access Journals (Sweden)

    I. Sugoro

    2015-04-01

    Full Text Available The viability of probiotics is important to be determined, as is its probiotic potency in the small instestine of fish. The result can be used as a basis to determine the feeding frequency of the probiotics to the fish.The aim of this study is to gain information about the viability of lactic acid bacteria (LAB in the small intestine of fish by using the 32P isotope technique. Catfish (Pangasius djambal was used as a test fish, and the LAB with the code of P2.1 PTB was the subject of the experiment. Before its viability was tested, the LAB had been labelled with radioisotope 32P, then mixed into catfish feed. Its viability could be determined by counting the activity of 32P. The results showed that the percentage of LAB viability in the small intestine of catfish declined until day 7. The percentage of LAB viability was decreased at an amount of 30% at day 3. Based on this result, the feeding frequency of LAB P2.1 PTB is every 3 days.

  3. Determination of Lactic Acid Bacteria Viability in the Small Intestine of Catfish (Pangasius djambal by Using the 32P Radioisotope

    Directory of Open Access Journals (Sweden)

    I. Sugoro

    2015-10-01

    Full Text Available The viability of probiotics is important to be determined, as is its probiotic potency in the small instestine of fish. The result can be used as a basis to determine the feeding frequency of the probiotics to the fish.The aim of this study is to gain information about the viability of lactic acid bacteria (LAB in the small intestine of fish by using the 32P isotope technique. Catfish (Pangasius djambal was used as a test fish, and the LAB with the code of P2.1 PTB was the subject of the experiment. Before its viability was tested, the LAB had been labelled with radioisotope 32P, then mixed into catfish feed. Its viability could be determined by counting the activity of 32P. The results showed that the percentage of LAB viability in the small intestine of catfish declined until day 7. The percentage of LAB viability was decreased at an amount of 30% at day 3. Based on this result, the feeding frequency of LAB P2.1 PTB is every 3 days. Received: 04 October 2014 Revised: 26 March 2015; Accepted: 05 April 2015

  4. Dietary beta-1,3 glucan potentiates innate immunity and disease resistance of Asian catfish, Clarias batrachus (L.).

    Science.gov (United States)

    Kumari, J; Sahoo, P K

    2006-02-01

    This study investigated the effects of short and prolonged administration of a yeast beta-glucan on non-specific immune parameters, growth rate and the disease resistance of Asian catfish, Clarias batrachus. Fish fed with a basal diet (control) and test diet (basal diet supplemented with 0.1% glucan) for 1, 2 and 3 weeks were assayed for superoxide production, serum myeloperoxidase (MPO) content, natural haemagglutinin level, complement and lysozyme activities. Fish were weighed at weekly intervals and specific growth rate (SGR, % increase in body weight per day) was determined. After each week, fish were challenged with Aeromonas hydrophila to measure the level of protection. Results showed that glucan administration at 0.1% in feed, significantly (Pcomplement activity and SGR were not affected by the dietary supplementation of yeast glucan. As glucan feeding at 0.1% for 1 week is able to enhance the non-specific immunity and disease resistance of catfish efficiently, short-term feeding might be used in farmed catfish diets to enhance disease resistance.

  5. Partial replacement of protein in soybean meal by moringa seed cake (Moringa oleifera in bocourti’s catfish (Pangasius bocourti

    Directory of Open Access Journals (Sweden)

    Bundit Yuangsoi

    2014-04-01

    Full Text Available The present study was undertaken in order to determine the effect of a dietary of moringa seed cake on digestibility, growth performance, blood chemistry and histopathologic of bocourti’s catfish. Fish were fed with diets formulated by 0, 250, 500, 750, and 1000 g kg-1 of moringa seed cake to replace protein in soybean meal. Fish with mean wet weights of 21.50± 0.25 g per fish were fed experimental diets for 8 weeks. Significant differences (p<0.05 in weight gain, average daily gain and specific growth rate were detected between bocourti’s catfish given the experimental diets. All fish grew normally and no significant difference was observed for survival rate and feed conversion ratio among fish fed tested diets. The highest FCR was generally observed that as moringa seed cake inclusion increased in the diets that were noted to exhibit slightly poor growth performance, feed utilization and pepsin digestibility tested. Blood chemistry and hepatosomatic index did not differ significantly for any of the diet treatments. No histopathological changes were found in distal intestines and liver. The study indicated that the dietary moringa seed cake contains ingredients that could be used for bocourti’s catfish diets possibly not over up to for 500 g kg-1 soybean protein replacement without negative effect on growth, digestibility and histology.

  6. Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus.

    Science.gov (United States)

    Zhao, Honggang; Li, Chao; Beck, Benjamin H; Zhang, Ran; Thongda, Wilawan; Davis, D Allen; Peatman, Eric

    2015-10-01

    One of the highest priority areas for improvement in aquaculture is the development of dietary additives and formulations which provide for complete mucosal health and protection of fish raised in intensive systems. Far greater attention has been paid to dietary impact on gut health than to protective effects at other mucosal surfaces such as skin and gill. These exterior surfaces, however, are important primary targets for pathogen attachment and invasion. Flavobacterium columnare, the causative agent of columnaris disease, is among the most prevalent of all freshwater disease-causing bacteria, impacting global aquaculture of catfish, salmonids, baitfish and aquaria-trade species among others. This study evaluated whether the feeding of a standard catfish diet supplemented with Alltech dietary additives Actigen(®), a concentrated source of yeast cell wall-derived material and/or Allzyme(®) SSF, a fermented strain of Aspergillus niger, could offer protection against F. columnare mortality. A nine-week feeding trial of channel catfish fingerlings with basal diet (B), B + Allzyme(®) SSF, B + Actigen(®) and B + Actigen(®)+Allzyme(®) SSF revealed good growth in all conditions (FCR additives may provide protection extending beyond the gut to surface mucosa. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. Determination of lactic acid bacteria viability in the small intestine of catfish (Pangasius Djambal) by using the 32P radioisotope

    International Nuclear Information System (INIS)

    Sugoro, I.; Citraresmini, A.; Murni, A.P.; Fairuz, D.

    2015-01-01

    The viability of probiotics is important to be determined, as is its probiotics potency in the small intestine of fish. The result can be used as a basis to determine the feeding frequency of the probiotics to the fish. The aim of this study is to gain information about the viability of lactic acid bacteria (LAB) in the small intestine of fish by using the 32 P isotope technique. Catfish (Pangasius djambal) was used as a test fish, and the LAB with the code of P2.1 PTB was the subject of the experiment. Before its viability was tested, the LAB had been labelled with radioisotope 32 P, then mixed into catfish feed. Its viability could be determined by counting the activity of 32 P. The results showed that the percentage of LAB viability in the small intestine of catfish declined until day 7. The percentage of LAB viability was decreased at an amount of 30% at day 3. Based on this result, the feeding frequency of LAB P2.1 PTB is every 3 days. (author)

  8. Applications of the Addition of extract and cinnamon leaf flour in the Diet on the Quality of Meat of Catfish

    Directory of Open Access Journals (Sweden)

    Suardi Laheng

    2016-04-01

    Full Text Available This study aimed to evaluate the effect of extract and cinnamon (Cinnamomum burmannii leaf flour in the diet in increasing quality of meat of catfish (Pangsianodon hypopthalmus. Catfish with a weight of 319.64 ± 35.99 g/nets reared in 9 nets with dimension 2x1x1,5 m3 at a density of 15 fish/nets for 60 days of maintenance. The fish were fed with leaves of cinnamon at a dose that was: 0% cinnamon leaf, 0.1% cinnamon leaf extract, and 1% cinnamon leaf flour. The fish were fed 2 times a day with the feeding rate of 3% of the average weight of the body. The results showed the treatment of leaf extract and flour, cinnamon capable of decreasing levels of body fat, fat meat by 10,31-12,27%, 37,26-50,23%, respectively, compared to controls (p<0,05, however, cinnamon leaf extract treatment is more effective in improving the quality of meat catfish that looks meat texture compact, white flesh color and a slightly sweet taste.

  9. Applications of the Addition of extract and cinnamon leaf flour in the Diet on the Quality of Meat of Catfish

    Directory of Open Access Journals (Sweden)

    Suardi Laheng

    2016-04-01

    Full Text Available This study aimed to evaluate the effect of extract and cinnamon (Cinnamomum burmannii leaf flourin the diet in increasing quality of meat of catfish (Pangsianodon hypopthalmus. Catfish with a weight of319.64 ± 35.99 g/nets reared in 9 nets with dimension 2x1x1,5 m3 at a density of 15 fish/nets for 60 daysof maintenance. The fish were fed with leaves of cinnamon at a dose that was: 0% cinnamon leaf, 0.1%cinnamon leaf extract, and 1% cinnamon leaf flour. The fish were fed 2 times a day with the feeding rate of3% of the average weight of the body. The results showed the treatment of leaf extract and flour, cinnamoncapable of decreasing levels of body fat, fat meat by 10,31-12,27%, 37,26-50,23%, respectively, compared tocontrols (p<0,05, however, cinnamon leaf extract treatment is more effective in improving the quality ofmeat catfish that looks meat texture compact, white flesh color and a slightly sweet taste.Keywords: cinnamon leaf, meat quality, Pangsianodon hypopthalmus

  10. Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens.

    Science.gov (United States)

    Iwanowicz, Luke R; Stafford, James L; Patiño, Reynaldo; Bengten, Eva; Miller, Norman W; Blazer, Vicki S

    2014-09-01

    Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that channel catfish (Ictalurus punctaus) leukocytes express ERα and ERβ2. Transcripts of these isoforms were detected in tissue-associated leukocyte populations by PCR, but ERβ2 was rarely detected in PBLs. Expression of these receptors was temporally regulated in PBLs following polyclonal activation by concanavalin A, lipopolysaccharide or alloantigen based on evaluation by quantitative and end-point PCR. Examination of long-term leukocyte cell lines demonstrated that these receptors are differentially expressed depending on leukocyte lineage and phenotype. Expression of ERs was also temporally dynamic in some leukocyte lineages and may reflect stage of cell maturity. Estrogens affect the responsiveness of channel catfish peripheral blood leukocytes (PBLs) to mitogens in vitro. Similarly, bactericidal activity and phorbol 12-myristate 13-acetate induced respiratory burst was modulated by 17β-estradiol. These actions were blocked by the pure ER antagonist ICI 182780 indicating that response is, in part, mediated via ERα. In summary, estrogen receptors are expressed in channel catfish leukocytes and participate in the regulation of the immune response. This is the first time leukocyte lineage expression has been reported in teleost cell lines. Published by Elsevier Ltd.

  11. Goliath catfish spawning in the far western Amazon confirmed by the distribution of mature adults, drifting larvae and migrating juveniles.

    Science.gov (United States)

    Barthem, Ronaldo B; Goulding, Michael; Leite, Rosseval G; Cañas, Carlos; Forsberg, Bruce; Venticinque, Eduardo; Petry, Paulo; Ribeiro, Mauro L de B; Chuctaya, Junior; Mercado, Armando

    2017-02-06

    We mapped the inferred long-distance migrations of four species of Amazonian goliath catfishes (Brachyplatystoma rousseauxii, B. platynemum, B. juruense and B. vaillantii) based on the presence of individuals with mature gonads and conducted statistical analysis of the expected long-distance downstream migrations of their larvae and juveniles. By linking the distribution of larval, juvenile and mature adult size classes across the Amazon, the results showed: (i) that the main spawning regions of these goliath catfish species are in the western Amazon; (ii) at least three species-B. rousseauxii, B. platynemum, and B. juruense-spawn partially or mainly as far upstream as the Andes; (iii) the main spawning area of B. rousseauxii is in or near the Andes; and (iv) the life history migration distances of B. rousseauxii are the longest strictly freshwater fish migrations in the world. These results provide an empirical baseline for tagging experiments, life histories extrapolated from otolith microchemistry interpretations and other methods to establish goliath catfish migratory routes, their seasonal timing and possible return (homing) to western headwater tributaries where they were born.

  12. The effects of diets containing standard soybean oil, soybean oil enhanced with conjugated linoleic acids, menhaden fish oil, or an algal docosahexaenoic acid supplement on juvenile channel catfish performance, hematology

    Science.gov (United States)

    Current commercial diets for Channel Catfish contain little or no marine fish oil to reduce diet cost and address environmental concerns. However, there is conflicting data on the effects of fish oil and other lipid sources in juvenile Channel Catfish, and some novel lipids have not been tested agai...

  13. Environmental comparison of intensive and integrated agriculture-aquaculture systems for striped catfish production in the Mekong Delta, Vietnam, based on two existing case studies using life cycle assessment

    NARCIS (Netherlands)

    Kluts, I.N.; Potting, J.M.B.; Bosma, R.H.; Phong, L.T.; Udo, H.M.J.

    2012-01-01

    Vietnam is the largest producer for the export of striped catfish. Traditionally striped catfish production in the Mekong Delta took place in integrated agriculture–aquaculture systems, but has shifted recently to intensive systems to meet increasing export demands. A recent study quantified the

  14. Determination of quarantine period in African catfish (Clarias gariepinus) fed with pig (Sus sp.) offal to assure compliance with halal standards.

    Science.gov (United States)

    Wan Norhana, M N; Dykes, G A; Padilah, B; Ahmad Hazizi, A A; Masazurah, A R

    2012-12-01

    Pig (Sus sp.) and pig by-products are considered as najasa (impurities) in Islam and forbidden in Muslim consumer products. Animals fed on najasa are categorised as al-jallālah (contaminated animals) which are allowed to be consumed as long as they have been quarantined for a certain period of time. During this quarantine period the animals will have undergone a natural purification process or istihālah. African catfish (Clarias gariepinus) are commonly consumed in Malaysia and may be fed on najasa. This study was carried out to estimate the istihālah period for catfish after feeding with pig offal, based on the absence of pig DNA in catfish gut and to suggest the quarantine period in catfish fed with pig offal. The results indicated that the maximum istihālah period could reach 36h in the stomach, 6h in the midgut and less than 2h in the hindgut although in many cases shorter periods were observed. Based on these results it is estimated that the minimum quarantine period for catfish fed with pig offal is 1.5days. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. Isolation and Identification of Aeromonas hydrophila and Saprolegnia sp. on Catfish (Clarias gariepinus) in Floating cages in Bozem Moro Krembangan Surabaya

    Science.gov (United States)

    Kusdarwati, Rahayu; Kismiyati; Sudarno; Kurniawan, Hendi; Teguh Prayogi, Yudha

    2017-02-01

    Catfish (Clarias gariepinus) is one of the familiar freshwater fish cultured in Indonesia farmer. One of the reason is the high mortality of the catfish infected by Aeromonas hydrophila and Saprolegnia sp. Motile Aeromonas septicemia (MAS) is a common bacterial disease, caused by Aeromonas hydrophila, which affects freshwater fish. In Southeast Asia, the outbreak of this disease was firstly reported from West Java in 1980, when a total of 82.5 tons a month of catfish were lost, while in Central Java in 1984, the total loss was 1.6 tons. Saprolegniosis can cause economic loss due to high mortality from its case reaching 10% to 50%. This research aimed to identify and determine the percentage of A. hydrophila and Saprolegnia sp. the catfish farmed in bozem Moro Krembangan, Surabaya, East Java. Meanwhile, a supporting parameter in this research is the value of water quality parameter including pH, temperature, ammonia and dissolved oxygen that were measured during sampling. The results showed that of the 20 samples taken from the two cages, 19 fish were positively infected by A. drophila. percentage of infections of A. hydrophila that infect umbo catfish in Moro Krembangan, was 95%, while the percentage Saprolegnia sp. was 90%.

  16. Experimental induction of motile Aeromonas septicemia in channel catfish (Ictalurus punctatus by waterborne challenge with virulent Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    Dunhua Zhang

    2016-05-01

    Full Text Available Motile Aeromonas septicemia (MAS, caused by virulent clonal isolates of Aeromonas hydrophila (vAh, is emerging as a major disease in catfish (Ictalurus punctatus aquaculture in the Southeastern United States. Predisposing conditions leading to vAh infection in catfish were however largely unknown. The objective of this study was to investigate factors that predispose catfish to vAh infection and establish a waterborne challenge model that mimics natural occurrence of MAS. Results of this study indicated that wounding on the fish body surface was one of the key factors that predisposed catfish to vAh infection via waterborne route. Relatively uniform wounds were created by clipping part of the fish adipose fin. Adipose fin clipped (Af-clipped fish behaved normally in terms of swimming and feeding and no mortality occurred in the control treatment (a mock challenge. When subjected to challenge in vAh-infected water, Af-clipped fish were highly susceptible, showing typical symptoms of MAS observed in the field. The mortality rate of Af-clipped fish was significantly associated with vAh concentration, challenge time and water temperature. About 90% mortality occurred within 48 h when Af-clipped fish were challenged for 1 h with vAh at the concentration of 2 × 107 colony forming units per mL of water (27 ± 1 °C. The waterborne challenge model was further tested using four field isolates including A. hydrophila and A. veronii. All vAh isolates caused about 90% mortality of Af-clipped fish and one isolate of Aeromonas veronii caused no mortality under the same challenge conditions. The waterborne challenge model described in this study would facilitate urgently-needed studies of MAS prevention (such as wound avoidance and healing and control (such as prophylactic vaccination; antibiotics treatment and probiotics screening. Keywords: Adipose fin, Channel catfish, Motile Aeromonas septicemia, Virulent Aeromonas hydrophila, Waterborne challenge

  17. Effects of dietary Tenebrio molitor meal on the growth performance, immune response and disease resistance of yellow catfish (Pelteobagrus fulvidraco).

    Science.gov (United States)

    Su, Jingzhi; Gong, Yulong; Cao, Shenping; Lu, Fei; Han, Dong; Liu, Haokun; Jin, Junyan; Yang, Yunxia; Zhu, Xiaoming; Xie, Shouqi

    2017-10-01

    This study evaluated the influence of diets containing mealworm (Tenebrio molitor) meal in partial substitution of fishmeal on growth performance and immune responses of juvenile yellow catfish (Pelteobagrus fulvidraco). Four diets were formulated to contain 0 (the control diet), 9, 18 and 27 g mealworm meal per 100 g diet with 0%, 25%, 50% and 75% of fishmeal replacement, respectively. Yellow catfish were randomly divided into 4 groups with 3 replicates in each group. The fish in each group were fed with one of the four experimental diets for 5 weeks. Growth performance, plasma parameters (SOD, MDA, IgM, C3, lysozyme) and immune related genes (MHC II, IL-1, CypA, IgM, HE) of yellow catfish were determined at the end of the feeding trial, as well as 24 h post bacterial (Edwardsiella ictaluri) challenge. The present results showed that dietary inclusion of mealworm meal (MW) had no negative effects on the growth performance of the juvenile yellow catfish, compared to the control group. At the end of the feeding trial, plasma MDA contents of MW supplemented groups were significant lower than the control group. Plasma SOD activities increased significantly with the increasing dietary MW contents at the end of feeding trial (pre-challenge) and 24 h post challenge with E. ictaluri. Significant increase of plasma lysozyme activity was found in MW supplemented groups compared to the control group 24 h post bacterial challenge. Plasma IgM levels increased significantly with the increasing dietary MW contents at the end of feeding trial. Compared with the control group, the immune related genes of MHC II, IL-1, IgM and HE of the fish in the MW supplemented groups significantly upregulated pre-challenge or 24 h post bacterial challenge. Finally, it was observed that the survival rate of the 27% MW group was significant higher (P < 0.05) than the control group but was not significantly differed from the 18% MW group. The present results indicated that dietary

  18. sharptooth catfish

    African Journals Online (AJOL)

    2010-02-28

    Feb 28, 2010 ... metal toxicity (Muramoto, 1980; James et al., 1998). Effects of ...... non-nutritive molecules (Hopson and Wessels, 1990; Sorensen,. 1991; Kimball, 1983) ..... DURAL M, GÖKSU MZL and ÖZAK AA (2007) Investigation of heavy metal ... HEM JD (1977) Reactions of metal ions at surfaces of hydrous iron oxide.

  19. Endosulfan and flutamide impair testicular development in the juvenile Asian catfish, Clarias batrachus

    Energy Technology Data Exchange (ETDEWEB)

    Rajakumar, A.; Singh, R.; Chakrabarty, S.; Murugananthkumar, R.; Laldinsangi, C.; Prathibha, Y.; Sudhakumari, C.C.; Dutta-Gupta, A. [Department of Animal Sciences, School of Life Sciences-Centre for Advanced Studies, University of Hyderabad, P. O. Central University, Hyderabad 500046, Andhra Pradesh (India); Senthilkumaran, B., E-mail: bsksl@uohyd.ernet.in [Department of Animal Sciences, School of Life Sciences-Centre for Advanced Studies, University of Hyderabad, P. O. Central University, Hyderabad 500046, Andhra Pradesh (India)

    2012-04-15

    Highlights: Black-Right-Pointing-Pointer Endosulfan and/or flutamide retard(s) testicular differentiation in juvenile fish. Black-Right-Pointing-Pointer Endosulfan and/or flutamide target(s) cfGnRH-Tph2 axis, either directly or indirectly. Black-Right-Pointing-Pointer Effects of endosulfan and/or flutamide on plasma androgen levels. Black-Right-Pointing-Pointer Compared changes in testis-related transcripts with testis germ cell distribution. Black-Right-Pointing-Pointer Our study on endosulfan endorse its prompt and complete phase-out/ban. - Abstract: Endosulfan and flutamide, a widely used pesticide and a prostate cancer/infertility drug, respectively, have an increased risk of causing endocrine disruption if they reach water bodies. Though many studies are available on neurotoxicity/bioaccumulation of endosulfan and receptor antagonism of flutamide, only little is known about their impact on testicular steroidogenesis at molecular level. Sex steroids play an important role in sex differentiation of lower vertebrates including fishes. Hence, a small change in their levels caused by endocrine disruptors affects the gonadal development of aquatic vertebrates significantly. The aim of this study was to evaluate the effects of endosulfan and flutamide on testis-related transcription factor and steroidogenic enzyme genes with a comparison on the levels of androgens during critical period of catfish testicular development. We also analyzed the correlation between the above-mentioned genes and catfish gonadotropin-releasing hormone (cfGnRH)-tryptophan hydroxylase2 (tph2). The Asian catfish, Clarias batrachus males at 50 days post hatch (dph) were exposed to very low dose of endosulfan (2.5 {mu}g/L) and flutamide (33 {mu}g/L), alone and in combination for 50 days. The doses used in this study were far less than those used in the previous studies of flutamide and reported levels of endosulfan in surface water and sediments. Sampling was done at end of the treatments (100

  20. Growth and survival of silver catfish larvae, Rhamdia quelen (Heptapteridae, at different calcium and magnesium concentrations

    Directory of Open Access Journals (Sweden)

    Lenise Vargas Flores da Silva

    Full Text Available Since the relative ratios of Ca2+ and Mg2+ can vary greatly from one water body to another, and lime used for the increase of water hardness or pH can have different ratios of Ca2+ and Mg2+ in its composition, the objective of this study was to analyze the growth and survival of silver catfish, Rhamdia quelen, larvae at different calcium and magnesium concentrations. After fertilization, eggs were randomly divided into 4 treatments (three replicates per treatment with different concentrations of Ca2+ and Mg2+ at hardness values of 70 mg.L-1 CaCO3 (mg.L-1 : 5.2 Ca2+ and 14.12 Mg2+; 13.11 Ca2+ and 7.11 Mg2+; 20.26 Ca2+ and 2.86 Mg2+; 24.95 Ca2+ and 0.95 Mg2+ and 150 mg.L-1 CaCO3 (mg.L-1 : 5.2 Ca2+ and 32.70 Mg2+; 28.63 Ca2+ and 16.44 Mg2+; 44.68 Ca2+and 6.44 Mg2+; 62.78 Ca2+ and 0.95 Mg2+. There was also another group exposed to water hardness of 20 mg.L-1 CaCO3 (Ca2+ 5.2 mg.L-1 and Mg2+ 0.95 mg.L-1 at both experiments. The post-hatch larvae were transferred to continuously aerated 40 L polyethylene aquaria (400 larvae/tank containing the same water as used for incubation. Samples of larvae were collected on days 0, 7, 14, and 21, and the length, weight, and specific growth rate were determined for each collection. Survival and biomass were calculated on day 21. At water hardness of 70 mg.L-1 CaCO3, the best survival and growth of silver catfish larvae was observed at water with 20.26 mg.L-1 Ca2+ and 2.89 mg.L-1 Mg2+, with similar results to the group exposed to water hardness of 20 mg.L-1 CaCO3. However, compared to the group exposed to water hardness of 20 mg.L-1 CaCO3, survival and growth were lower at 150 mg.L-1 CaCO3. Therefore, a hardness range of 20 to 70 mg.L-1 CaCO3 is recommended for silver catfish larviculture, but with 20.26 mg.L-1 Ca2+ and 2.89 mg.L-1 Mg2+ at 70 mg.L-1 CaCO3. Water hardness of 150 mg.L-1 CaCO3 is not recommended for this species.

  1. A deletion in the Hermansky-Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish.

    Science.gov (United States)

    Li, Yueru; Geng, Xin; Bao, Lisui; Elaswad, Ahmed; Huggins, Kevin W; Dunham, Rex; Liu, Zhanjiang

    2017-06-01

    Albinism is caused by a series of genetic abnormalities leading to reduction of melanin production. Albinism is quite frequent in catfish, but the causative gene and the molecular basis were unknown. In this study, we conducted a genome-wide association study (GWAS) using the 250 K SNP array. The GWAS analysis allowed mapping of the albino phenotype in the Hermansky-Pudlak syndrome 4 (Hps4) gene, which is known to be involved in melanosome biosynthesis. Sequencing analysis revealed that a 99-bp deletion was present in all analyzed albino catfish at the intron 2 and exon 3 junction. This deletion led to the skipping of the entire exon 3 which was confirmed by RT-PCR. Therefore, Hps4 was determined to be the candidate gene of the catfish albinism.

  2. POTENTIAL FOR GREAT EGRETS (ARDEA ALBA) TO TRANSMIT A VIRULENT STRAIN OF AEROMONAS HYDROPHILA AMONG CHANNEL CATFISH (ICTALURUS PUNCTATUS) CULTURE PONDS.

    Science.gov (United States)

    Jubirt, Madison M; Hanson, Larry A; Hanson-Dorr, Katie C; Ford, Lorelei; Lemmons, Scott; Fioranelli, Paul; Cunningham, Fred L

    2015-07-01

    Aeromonas hydrophila is a gram-negative, rod-shaped, facultative, anaerobic bacterium that is ubiquitous in freshwater and slightly brackish aquatic environments and infects fish, humans, reptiles, and birds. Recent severe outbreaks of disease in commercial channel catfish (Ictalurus punctatus) aquaculture ponds have been associated with a highly virulent A. hydrophila strain (VAH), which is genetically distinct from less-virulent strains. The epidemiology of this disease has not been determined. Given that A. hydrophila infects birds, we hypothesized that fish-eating birds may serve as a reservoir for VAH and spread the pathogen by flying to uninfected ponds. Great Egrets (Ardea alba) were used in this transmission model because these wading birds frequently prey on farmed catfish. Great Egrets that were fed VAH-infected catfish shed VAH in feces demonstrating their potential to spread VAH.

  3. Histological development of the digestive system of the Amazonian pimelodid catfish Pseudoplatystoma punctifer.

    Science.gov (United States)

    Gisbert, E; Moreira, C; Castro-Ruiz, D; Oztürk, S; Fernández, C; Gilles, S; Nuñez, J; Duponchelle, F; Tello, S; Renno, J F; García-Dávila, C; Darias, M J

    2014-11-01

    The organogenesis of the digestive system was described in the Amazonian pimelodid catfish species Pseudoplatystoma punctifer from hatching (3.5 mm total length, TL) to 41 days post-fertilization (dpf) (58.1 mm TL) reared at 28°C. Newly hatched larvae showed a simple digestive tract, which appeared as a straight undifferentiated and unfolded tube lined by a single layer of columnar epithelial cells (future enterocytes). During the endogenous feeding period, comprised between 20 and 96 h post-fertilization (3.5 to 6.1 mm TL), the larval digestive system experienced a fast transformation with the almost complete development and differentiation of most of digestive organs (buccopahrynx, oesophagus, intestine, liver and exocrine pancreas). Yolk reserves were not completely depleted at the onset of exogenous feeding (4 dpf, 6.1 mm TL), and a period of mixed nutrition was observed up to 6 to 7 dpf (6.8 to 7.3 mm TL) when yolk was definitively exhausted. The stomach was the organ that latest achieved its complete differentiation, characterized by the development of abundant gastric glands in the fundic stomach between 10 and 15 dpf (10.9 to 15.8 mm TL) and the formation of the pyloric sphincter at the junction of the pyloric stomach and the anterior intestine at 15 dpf (15.8 mm TL). The above-mentioned morphological and histological features observed suggested the achievement of a digestive system characteristic of P. punctifer juveniles and adults. The ontogeny of the digestive system in P. punctifer followed the same general pattern as in most Siluriform species so far, although some species-specific differences in the timing of differentiation of several digestive structures were noted, which might be related to different reproductive guilds, egg and larval size or even different larval rearing practices. According to present findings on the histological development of the digestive system in P. punctifer, some recommendations regarding the rearing practices of this

  4. Lens proteome map and alpha-crystallin profile of the catfish Rita rita.

    Science.gov (United States)

    Mohanty, Bimal Prasanna; Bhattacharjee, Soma; Das, Manas Kumar

    2011-02-01

    Crystallins are a diverse group of proteins that constitute nearly 90% of the total soluble proteins of the vertebrate eye lens and these tightly packed crystallins are responsible for transparency of the lens. These proteins have been studied in different model and non-model species for understanding the modifications they undergo with ageing that lead to cataract, a disease of protein aggregation. In the present investigation, we studied the lens crystallin profile of the tropical freshwater catfish Rita rita. Profiles of lens crystallins were analyzed and crystallin proteome maps of Rita rita were generated for the first time. alphaA-crystallins, member of the alpha-crystallin family, which are molecular chaperons and play crucial role in maintaining lens transparency were identified by 1- and 2-D immunoblot analysis with anti-alphaA-crystallin antibody. Two protein bands of 19-20 kDa were identified as alphaA-crystallins on 1-D immunoblots and these bands separated into 10 discrete spots on 2-D immunoblot. However, anti-alphaB-crystallin and antiphospho-alphaB-crystallin antibodies were not able to detect any immunoreactive bands on 1- and 2-D immunoblots, indicating alphaB-crystallin was either absent or present in extremely low concentration in Rita rita lens. Thus, Rita rita alpha-crystallins are more like that of the catfish Clarias batrachus and the mammal kangaroo in its alphaA- and alphaB-crystallin content (contain low amount from 5-9% of alphaB-crystallin) and unlike the dogfish, zebrafish, human, bovine and mouse alpha-crystallins (contain higher amount of alphaB-crystallin from 25% in mouse and bovine to 85% in dogfish). Results of the present study can be the baseline information for stimulating further investigation on Rita rita lens crystallins for comparative lens proteomics. Comparing and contrasting the alpha-crystallins of the dogfish and Rita rita may provide valuable information on the functional attributes of alphaA- and alphaB-isoforms, as

  5. Efeito da estratégia de vida sobre as variações no conteúdo de energia de duas espécies de peixes (Brycon hilarii e Hypophthalmus edentatus, durante o ciclo reprodutivo - DOI: 10.4025/actascibiolsci.v29i2.521 Effect of life strategy on the variations of energy content of two fish species (Brycon hilarii e Hypophthalmus edentatus during their reproductive cycle - DOI: 10.4025/actascibiolsci.v29i2.521

    Directory of Open Access Journals (Sweden)

    Evanilde Benedito

    2007-12-01

    Full Text Available Com o objetivo de investigar diferenças no conteúdo de energia durante o ciclo reprodutivo, foram selecionados 54 exemplares de Brycon hilarii (k-estrategista e 82 de Hypophthalmus edentatus (r-estrategista em amostragens realizadas mensalmente, entre outubro de 2000 e março de 2004, no reservatório de Manso, Estado do Mato Grosso do Norte e em sua área de influência. Determinou-se, para cada indivíduo, o conteúdo calórico, em kcal g-1 de peso seco, com auxílio da bomba calorimétrica (Parr 1261 e, realizaram-se comparações entre os valores calóricos obtidos, o fator de condição (K e a relação gonadossomática. Observou-se que, em geral, os valores médios de K e do conteúdo calórico foram superiores nos machos das duas espécies analisadas, embora não tenham sido identificadas diferenças significativas entre os sexos. Constatou-se que os valores calóricos de H. edentatus foram superiores aos de B. hilarii, enquanto os valores do fator de condição foram contrários, apresentando-se superiores para B. hilarii. Diferenças significativas foram constatadas nas comparações realizadas entre o conteúdo energético e o fator de condição, de indivíduos de ambas as espécies. Enquanto B. hilarii apresentou as mais elevadas concentrações de energia nos músculos, durante o máximo desenvolvimento gonadal, em H. edentatus, isso ocorreuquando iniciava o processo de maturação gonadal. Os resultados descritos para cada espécie durante o ciclo reprodutivo permitem confirmar a hipótese de que as duas espécies de peixes, B. hilarii e H. edentatus, com diferentes estratégias de vida, acumulam e mobilizam energia de forma distinta durante o período de maturação gonadal.The aim of this work was to investigate differences in the energy content during reproductive cycle of two species of fish with different life strategies: Brycon hilarii (k-strategist and Hypophthalmus edentatus (r-strategist. Fifty-four samples of B. hilarii

  6. Determination of niclosamide residues in rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus) fillet tissue by high-performance liquid chromatography

    Science.gov (United States)

    Schreier, Theresa M.; Dawson, V.K.; Cho, Yirang; Spanjers, N.J.; Boogaard, M.A.

    2000-01-01

    Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combination with 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributary to the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed for the determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM during sea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow trout fillet tissue with a series of acetone extractions and cleaned up on C-18 solid-phase extraction cartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm for rainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04 mu g/g was 77 +/- 6.5% and from channel catfish (n = 7) fortified at 0.02 mu g/g was 113 +/- 11%. NIC detection limit was 0.0107 mu g/g for rainbow trout and 0.0063 mu g/g for catfish. Percent recovery of incurred radioactive residues by this method from catfish exposed to [C-14]NIC was 89.3 +/- 4.1%. Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3) analyzed at 5 and 7.5 month periods were 78 +/- 5.1 and 68 +/- 2.4%, respectively. Percent recoveries of NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and 7.5 month periods were 88 +/- 13 and 76 +/- 21%, respectively.

  7. Pemberian Pakan Yang Difermentasikan Dengan Probiotik Untuk Pemeliharaan Ikan Lele Dumbo (Clarias Gariepinus) Pada Teknologi Bioflok Feed Fermented Using Probiotic for African Catfish in Biofloc Technology

    OpenAIRE

    Yulianingrum, Triwahyu; Pamukas, Niken Ayu; Putra, Iskandar

    2017-01-01

    This study aimed to determine the effect of feeding fermented with probiotic on, absolute body weight, absolute body length, specific growth rate, survival, feed efficiency and feed conversion rate of African catfish preserved biofloc techniques. The treatment in this study was the dose used for the fermentation of feed are 2 ml / kg (P1), 4 ml / kg (P2), 6 ml / kg (P3), and control (feed without fermented). Each treatment was repeated 3 times. 4-5 cm catfish were kept in the plastic tank wit...

  8. Procamallanus (Spirocamallanus) chetumalensis n. sp. (Nematoda: Camallanidae) from the Mayan sea catfish, Ariopsis assimilis, off the Caribbean coast of México.

    Science.gov (United States)

    González-Solís, David; Moravec, Frantisek; Vidal-Martínez, Victor M

    2002-08-01

    Procamallanus (Spirocamallanus) chetumalensis n. sp. is described from the stomach and intestine of the Mayan sea catfish Ariopsis assimilis (Günther, 1864), from the Bay of Chetumal, Quintana Roo, México. It is characterized by bifurcate deirids; males have 3 pairs of preanal papillae, 6 pairs of postanal papillae, 2 pairs of transverse elongate adanal papillae surrounding the cloacal aperture, wide caudal alae, spicules of unequal length, and a gubernaculum, and females have a rounded tail bearing a digit-like process terminating in 2 spines. This is the seventh Procamallanus (Spirocamallanus) species reported from fishes in Mexico and the first one recorded in sea catfishes of the Ariidae.

  9. Records of the endemic and threatened catfish, Hemibagrus punctuates from the southern Western Ghats with notes on its distribution, ecology and conservation status

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    A. Ali

    2013-07-01

    Full Text Available The Nilgiri Mystus, Hemibagrus punctatus, a rare bagrid catfish endemic to the Western Ghats, has been currently listed in the IUCN Red List, as Critically Endangered with a possibility that it could be extinct. The last validated record of H. punctatus was known to be in 1998, and several surveys since then have not been able to collect the species from its native range. In this paper, we provide information on new records of this rare catfish from the Western Ghats after a period of 14 years, and discuss its distribution, ecology and conservation. An updated conservation assessment of this species following the IUCN Red List Criteria is also provided.

  10. Age and growth of the Duckbill Catfish (Sorubim cf. lima in the Pantanal

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    J. M. F. Penha

    Full Text Available The Duckbill Catfish, Sorubim lima, is a predator of large South American rivers. The age and growth of S. lima were studied based on the pectoral fin-spines of samples collected from the Cuiabá River, Pantanal. The samples were taken from commercial and experimental hook-and-line fishing. An analysis of the marginal increment suggests that the growth rings are formed once a year during the dry season, from July to September (ANOVA type I: F = 4.183; g.l. = 3 and 104; p = 0.008. The estimate of the parameters that describe von Bertalanffy's growth curve by nonlinear regression of the observed lengths in the age were: L¥ = 56.0 cm (fork length; k = 0.245 year-1; t o = -2.605 years. The animals were estimated to have a life span of 9.6 years. The findings indicate that the fork length is a good predictor of the age of individuals of this fish species.

  11. Sound production to electric discharge: sonic muscle evolution in progress in Synodontis spp. catfishes (Mochokidae).

    Science.gov (United States)

    Boyle, Kelly S; Colleye, Orphal; Parmentier, Eric

    2014-09-22

    Elucidating the origins of complex biological structures has been one of the major challenges of evolutionary studies. Within vertebrates, the capacity to produce regular coordinated electric organ discharges (EODs) has evolved independently in different fish lineages. Intermediate stages, however, are not known. We show that, within a single catfish genus, some species are able to produce sounds, electric discharges or both signals (though not simultaneously). We highlight that both acoustic and electric communication result from actions of the same muscle. In parallel to their abilities, the studied species show different degrees of myofibril development in the sonic and electric muscle. The lowest myofibril density was observed in Synodontis nigriventris, which produced EODs but no swim bladder sounds, whereas the greatest myofibril density was observed in Synodontis grandiops, the species that produced the longest sound trains but did not emit EODs. Additionally, S. grandiops exhibited the lowest auditory thresholds. Swim bladder sounds were similar among species, while EODs were distinctive at the species level. We hypothesize that communication with conspecifics favoured the development of species-specific EOD signals and suggest an evolutionary explanation for the transition from a fast sonic muscle to electrocytes. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

  12. Effect of artificial photoperiod on the blood cell indices of the catfish, Clarias batrachus

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    S Srivastava

    2010-04-01

    Full Text Available The present study is aimed to assess the influence of artificial photoperiod on the blood cell indices of an Indian catfish, Clarias batrachus(Linn.. Blood samples taken from adult fishes exposed to artificial photoperiod of 24L:0D and 0L:24D for a short period of 24 hrs, were analyzed for total RBC, total WBC, differential leukocyte count (DLC and some physiological variables – glucose and chloride. The total RBC and WBC counts were unaffected by both the artificial photoperiod regimes. However, lymphopenia (p<.05 and neutrophilia (p<.05 were observed under 24L:0D photoperiod. Blood chloride levels were significantly higher (p<.05 in 24L:0D whereas blood glucose levels remained unchanged in both the photoperiod treatments. The 0L:24D photoperiod did not produce any significant change in the blood cell indices neither in the physiological variables. The findings indicate that exposure to continuous light for as short a duration as of 24 hrs elicits stress responses in the leukocyte profile of this nocturnal fish and highlights the role of leukocyte profile as potential stress biomarkers in vertebrates.

  13. Stress and Welfare of African Catfish (Clarias gariepinus Burchell, 1822 in a Coupled Aquaponic System

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    Björn Baßmann

    2017-07-01

    Full Text Available African catfish (Clarias gariepinus were investigated for physiological stress parameters to assess the influence of different cultivation techniques on fish welfare. The fish were reared in two conceptually identical recirculating aquaculture systems (RAS constructed in a greenhouse. One system was combined with a floating raft hydroponic unit to culture cucumbers (Cucumis sativus only using the process water. The second system remained without the plant cultivation unit and functioned as the control. Water quality was monitored regularly by measuring dissolved nutrients and physical water parameters. During 87 days, a total of 71 fish from each system (n = 142 were studied, with a final sampling of all fish (n = 107 at the end of the experiment. Blood from the caudal vein was analyzed for the stress hormone cortisol and the glucose concentration. In addition, fish were investigated for external injuries on their skin, fins and barbels. The results demonstrate that the system design had no influence on fish mortalities and growth rates. Furthermore, cortisol and blood glucose levels did not differ significantly between the two systems. However, the number of external injuries was significantly lower in the aquaponics, providing evidence that co-cultivation of fish and plants might offer benefits to the welfare of the fish.

  14. Growth, feeding and reproduction of the catfish Eremophilus mutisii (Pisces: Trichomycteridae), from artificial reservoirs in Colombia.

    Science.gov (United States)

    Pinilla, Gabriel; Abril, Maritza; González, Esperanza

    2006-06-01

    The catfish, Eremophilus mutisii, was cultured under different densities in artificial rainfall reservoirs at Caldas, Boyacá, Colombia. At capture, the average total length of the animals was 10 cm and the weight ranged between 9 and 10 g. These fish were in initial stages of sexual development. After a year in the reservoirs, they had increased in length and weight as follows (densities in parentheses): 5.22 cm and 27.23 g (0.5 individuals/m2); 1.98 cm and 8.79 g (1 individual/m2), and 2.45 cm and 0.82 g (2 individuals/m2). They were at the early stages of sexual maturity, and their condition factor fluctuated between 0.61 and 0.96. They preferably ingested insect larvae that were found in benthic and littoral communities of the reservoirs. The benthos was dominated by ephippia of Daphnia, lumbriculids, and chironomid larvae. The biomass of benthic organisms ranged between 0.1126 and 1.3847 g/m2. In the littoral community aquatic insects were the most important component, especially the hemipterans. The biomass of the littoral fauna ranged between 0.14 and 4.05 g/m2.

  15. Impact of Red Water System (RWS) application on water quality of catfish culture using aquaponics

    Science.gov (United States)

    Zahidah; Dhahiyat, Y.; Andriani, Y.; Sahidin, A.; Farizi, I.

    2018-03-01

    This study aim was to analyze the effect of Red Water System (RWS) probiotics application on water quality in aquaponic system. The research used experimental method using Completely Randomized Design (CRD) with five treatments and three replications. Treatment A: RWS 7.5 μL·L-1/week without aquaponic probiotic, Treatment B: aquaponic without RWS probiotics, treatment C: RWS probiotic addition in aquaponic media at 7.5 μL·L-1/week, treatment D: addition of RWS probiotics in aquaponic media at 10 μL·L-1/week and treatment E: addition of RWS probiotics in in aquaponic media at 12.8 μL·L-1/week. Parameters measured were pH, temperature, ammonia, nitrate and phosphate. The results showed that water temperature and pH relatively unchanged in all treatments. The addition of RWS probiotics did not improve the concentration of ammonia, nitrate and phosphate. In fact, the catfish culture with only aquaponic resulted lower concentration of ammonia, nitrate and phosphate than other treatment. The lowest value of ammonia, nitrate and phosphates was obtained in the experimental groups of aquaponic with RWS of 10 μL·L-1/week (Treatment D). Treatment D has the lowest average ammonia of 0.50 ppm, reduced nitrate up to 60.78 % and temperature and pH relatively unchanged.

  16. Prediction of Toxin Genes from Chinese Yellow Catfish Based on Transcriptomic and Proteomic Sequencing

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    Bing Xie

    2016-04-01

    Full Text Available Fish venom remains a virtually untapped resource. There are so few fish toxin sequences for reference, which increases the difficulty to study toxins from venomous fish and to develop efficient and fast methods to dig out toxin genes or proteins. Here, we utilized Chinese yellow catfish (Pelteobagrus fulvidraco as our research object, since it is a representative species in Siluriformes with its venom glands embedded in the pectoral and dorsal fins. In this study, we set up an in-house toxin database and a novel toxin-discovering protocol to dig out precise toxin genes by combination of transcriptomic and proteomic sequencing. Finally, we obtained 15 putative toxin proteins distributed in five groups, namely Veficolin, Ink toxin, Adamalysin, Za2G and CRISP toxin. It seems that we have developed a novel bioinformatics method, through which we could identify toxin proteins with high confidence. Meanwhile, these toxins can also be useful for comparative studies in other fish and development of potential drugs.

  17. Ecological implications of floods on the parasite communities of two freshwater catfishes in a Neotropical floodplain.

    Science.gov (United States)

    Yamada, Priscilla de Oliveira Fadel; Yamada, Fabio Hideki; da Silva, Reinaldo José; Anjos, Luciano Alves Dos

    2017-06-01

    The parasite communities of two freshwater catfishes (Auchenipterus osteomystax and Trachelyopterus galeatus) were analyzed during the dry and rainy seasons in a Neotropical floodplain. The nature of river-floodplain systems places specific demands on parasite community structure, due to changes in the host environment. It was therefore hypothesized that flood conditions lead to an increase in the richness and abundance of fish parasites in the rainy season at the mouth of Aguapeí River floodplain. The Auchenipterus osteomystax parasite community was richer (11 vs. eight) in species than T. galeatus, although the latter exhibited a greater parasite burden (F1,108 = 126.99, PFlood conditions during the rainy season caused a change in the composition and structure of the parasitic communities. The results corroborate the hypothesis that floods are one of the most significant influences on shaping the parasite communities of fish in floodplains. Our greatest concern is the reduction these dynamics and effects bring about on local biota and, consequently, in host-parasite interaction. We would therefore like to take this opportunity to warn environmental agencies and hydroelectric companies about the importance of the conservation of the diversity of this location.

  18. A fossil loricariid catfish (Siluriformes: Loricarioidea from the Taubaté Basin, eastern Brazil

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    Maria Claudia Malabarba

    Full Text Available A new loricariid catfish is described from the Tremembé Formation (Late Oligocene to Early Miocene sediments of the Taubaté Basin in eastern São Paulo State, Brazil. Taubateia paraiba, new genus and species, is based on a single specimen preserved as a ventral-side impression of an articulated partial neurocranium, dorsal elements of the pectoral girdle and anterior vertebrae. The fossil is identified as belonging to family Loricariidae based on obvious overall similarity and the presence of diagnostic derived characters such as: odontodes, dorsal margin of metapterygoid contacting lateral ethmoid, presence of mesethmoid disk (condyle, and compound pterotic-supracleithrum bone. Also, as in most loricariids, the ossified transcapular (Baudelot's ligament plus basiocciptal lateral process form a prominent transverse wall at the occiput. Other derived characters preserved in Taubateia are synapomorphies at different levels within Loricariidae, including a wide and low parasphenoid, form of pterotic-supracleithrum, shape and position of the mesethmoid disk, a triangular lateral ethmoid with expanded posterolateral corner and a rounded and low ridge articulating with the metapterygoid, and a pointed distal margin of transverse process of the Weberian compound centrum. The derived characters recognized in this fossil are a distinctive combination for diagnosing a new genus and species but not for its unambiguous placements in any of the currently recognized loricariid subfamilies.

  19. Generation of viable progeny from dead brooders of endangered catfish Clarias magur (Hamilton, 1822

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    Sullip Kumar Majhi

    2017-10-01

    Full Text Available The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C. magur. Three hours after death, the gonads were excised, macroscopically examined and gamete viability was evaluated. Artificial fertilization was performed by mixing the sperm suspension with the eggs. Water was added after 1 min of mixing to activate the fertilization process. We observed 85%-93% fertilization success from gametes derived from dead donors as opposed to 90%-95% from those derived from live control donors. The embryos showed normal development and resulted in the generation of 88%-92% viable progeny, which was similar to the progeny derived from control donors (92%-93%. The results obtained in this study will have profound implications in enhancing the seed production of endangered C. magur and could potentially be applied to other key commercially or endangered fish species. Keywords: Biological sciences, Developmental biology, Zoology

  20. Ostracism of an albino individual by a group of pigmented catfish.

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    Ondřej Slavík

    Full Text Available Physiological and behavioural constraints hinder albino individuals. Albino animals are rare in the wild; this trait is associated with easy detection by predators, non-native or damaged environments, and exclusively aphotic environments in total darkness. The social aspect of albinism is reported only for human beings, and the effect is distinguishable in time and space when social benefits, are used to a limited the extent. Thus far, the social consequences of albinism for animals remain unknown. We used socially established groups of the pigmented catfish, (Silurus glanis, to observe space and temporal distance detachment of albino specimens in laboratory conditions. The albino fish were separated at larger distances from the group than pigmented individuals with the same social status determined by familiarity, and this asymmetry also varied in time. Albinism-related ostracism results in a solitary existence, usually followed by enhanced predation risk. The motivation for an individual's exclusion from a group appears to be the avoidance of the predation risk that increases not only for an odd individual but also for conspecifics within a group. Our findings indicate a role for albinism in behavioural processes related to sociality in a group of conspecifics.

  1. Otocinclus batmani, a new species of hypoptopomatine catfish (Siluriformes: Loricariidae from Colombia and Peru

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    Pablo Lehmann A.

    Full Text Available A new species of the hypoptopomatine catfish genus Otocinclus is described from two localities in the upper Río Amazonas basin: a tributary of the Río Puré in Colombia and two tributaries to the Río Amazonas near Iquitos in Peru. The new taxon can be easily distinguished from all congeners, except Otocinclus cocama, by having a single, intensely pigmented, vertical W-shaped caudal fin spot and by having three discrete dark bands on dorsum, between the dorsal-fin base and the caudal fin. Otocinclus batmani differs from O. cocama by the absence of vertically elongated blotches from the dorsal midline to the ventral border of flanks, and by lacking a posterior extension of black pigmentation on the base of two central caudal-fin rays. Phylogenetic relationships of the new species are investigated and it is possibly more closely related to a clade formed by O. huaorani, O. mariae, O. bororo, O. mura, and O. cocama.

  2. A novel male-associated marker for sex identification in Ussuri catfish Pseudobagrus ussuriensis

    Science.gov (United States)

    Zhu, Chuankun; Pan, Zhengjun; Wang, Hui; Chang, Guoliang; Ding, Huaiyu

    2017-08-01

    The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2-3 times faster than females. Therefore, all-male breeding could increase production of this fish, and sex-linked markers would be useful during the breeding process. In this study, a male-associated marker named PuGT54 was isolated in P. ussuriensis from a previously constructed microsatellite library. A total of 185 P. ussuriensis individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified, indicating the high reliability and accuracy of this marker for sex identification. Furthermore, the male-specific fragment amplified by this maker was cloned and sequenced, which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn, detailed information of the male-specific fragment is unknown presently. Nevertheless, this marker would be helpful in conservation, breeding and ecological study of P. ussuriensis.

  3. Health Food Supplements (Health Food Highly Nutritious From Chlorella And Oil Catfish (Pangasius hypopthalmus

    Directory of Open Access Journals (Sweden)

    Syahrul Syahrul

    2017-02-01

    Full Text Available AbstractThe utilization of microalgae as a food ingredient considered effective, because in addition to alternative food sources also contains nutrients chlorella microalgae in particular is very good for health. This microalgae rich in protein (60.5%, fat (11%, carbohydrates (20.1%, water, dietary fiber, vitamins and minerals Besides these microalgae contain pigments (chlorophyll, tocopherol and the active component (antimicrobial and antioxidants. This is what underlies microalgae is very useful to be used as a source of raw materials of health food supplements. Currently the health food supplements have become a necessity for people to maintain their health in order to remain vibrant. This study aims to produce high nutritious health food supplements from raw material chlorella enriched with fish protein concentrate and oil catfish. The method used in the manufacture of high nutritious health food supplement is a method of microencapsulation with different formulations. The results showed that the best formulations based on the profile of amino acids, fatty acids and standards AAE per day especially essential fatty acids oleic and linoleic is formulation B (chlorella 2%, 1% fish oil and fish protein concentrate 1%.

  4. Health Food Supplements (“Health Food” Highly Nutritious From Chlorella And Oil Catfish (Pangasius hypopthalmus

    Directory of Open Access Journals (Sweden)

    Syahrul Syahrul

    2016-12-01

    Full Text Available The utilization of microalgae as a food ingredient considered effective, because in addition to alternativefood sources also contains nutrients chlorella microalgae in particular is very good for health. This microalgaerich in protein (60.5%, fat (11%, carbohydrates (20.1%, water, dietary fiber, vitamins and minerals Besidesthese microalgae contain pigments (chlorophyll, tocopherol and the active component (antimicrobial andantioxidants. This is what underlies microalgae is very useful to be used as a source of raw materials ofhealth food supplements. Currently the health food supplements have become a necessity for people tomaintain their health in order to remain vibrant. This study aims to produce high nutritious health foodsupplements from raw material chlorella enriched with fish protein concentrate and oil catfish. The methodused in the manufacture of high nutritious health food supplement is a method of microencapsulation withdifferent formulations. The results showed that the best formulations based on the profile of amino acids,fatty acids and standards AAE per day especially essential fatty acids oleic and linoleic is formulation B(chlorella 2%, 1% fish oil and fish protein concentrate 1%.

  5. A new genus of Neotropical rheophilic catfishes, with four new species (Teleostei: Siluriformes: Pseudopimelodidae

    Directory of Open Access Journals (Sweden)

    Oscar Akio Shibatta

    2017-06-01

    Full Text Available ABSTRACT Rhyacoglanis, a new genus of the South American freshwater catfish family Pseudopimelodidae is described from cis-Andean portions of the continent. Rhyacoglanis is distinguished from other genera of the family by three synapomorphies: presence of a light blotch on the cheek; a connection between the middle of the dark caudal-fin stripe and the dark caudal-peduncle pigmentation; and 30-35 total vertebrae. Species of Rhyacoglanis are rheophilic and strongly associated with rapids and other swift-flowing waters. A phylogenetic analysis based on 41 morphological characters yields a hypothesis of monophyly of the Pseudopimelodidae and Rhyacoglanis. Pimelodus pulcher Boulenger, 1887, from the western Amazon basin is designated as type-species of the new genus and redescribed. Four new species are described: Rhyacoglanis annulatus, from the río Orinoco basin, with a nearly ringed dark band on the caudal peduncle, and a larger distance between anus and anal-fin origin; R. epiblepsis, from the rio Madeira basin, with numerous dark spots scattered on the body, and rounded caudal-fin lobes; R. paranensis, from the upper rio Paraná basin, with three distinct dark bands on the body, and 31-33 total vertebrae; and R. seminiger, from the rio Juruena basin, with subdorsal and subadipose dark bands fused anteroposteriorly, and a separate dark band on the caudal peduncle.

  6. Carbohydrate management, anaerobic metabolism, and adenosine levels in the armoured catfish, Liposarcus pardalis (castelnau), during hypoxia.

    Science.gov (United States)

    Maccormack, Tyson James; Lewis, Johanne Mari; Almeida-Val, Vera Maria Fonseca; Val, Adalberto Luis; Driedzic, William Robert

    2006-04-01

    The armoured catfish, Liposarcus pardalis, tolerates severe hypoxia at high temperatures. Although this species can breathe air, it also has a strong anaerobic metabolism. We assessed tissue to plasma glucose ratios and glycogen and lactate in a number of tissues under "natural" pond hypoxia, and severe aquarium hypoxia without aerial respiration. Armour lactate content and adenosine in brain and heart were also investigated. During normoxia, tissue to plasma glucose ratios in gill, brain, and heart were close to one. Hypoxia increased plasma glucose and decreased tissue to plasma ratios to less than one, suggesting glucose phosphorylation is activated more than uptake. High normoxic white muscle glucose relative to plasma suggests gluconeogenesis or active glucose uptake. Excess muscle glucose may serve as a metabolic reserve since hypoxia decreased muscle to plasma glucose ratios. Mild pond hypoxia changed glucose management in the absence of lactate accumulation. Lactate was elevated in all tissues except armour following aquarium hypoxia; however, confinement in aquaria increased armour lactate, even under normoxia. A stress-associated acidosis may contribute to armour lactate sequestration. High plasma lactate levels were associated with brain adenosine accumulation. An increase in heart adenosine was triggered by confinement in aquaria, although not by hypoxia alone.

  7. Tapeworms (Cestoda: Proteocephalidea) of firewood catfish Sorubimichthys planiceps (Siluriformes: Pimelodidae) from the Amazon River.

    Science.gov (United States)

    de Chambrier, Alain; Scholz, Tomás

    2008-03-01

    A survey of proteocephalidean cestodes found in the firewood catfish Sorubimichthys planiceps (Spix et Agassiz) from the Amazon River is provided. The following taxa parasitic in S. planiceps are redescribed on the basis of their type specimens and material collected recently in the Amazon River, near the type localities in Brazil, and in Iquitos, Peru: Monticellia lenha Woodland, 1933; Nomimoscolex lenha (Woodland, 1933) (syn. Proteocephalus lenha Woodland, 1933); and Monticellia megacephala Woodland, 1934, for which a new genus, Lenhataenia, is proposed, with L. megacephala (Woodland, 1934) comb. n. as its type and only species. The new genus is a member of the Monticelliinae, i.e. has all genital organs in the cortex, and is most similar to Chambriella in possessing biloculate suckers and lacking a metascolex. It differs in the morphology of the cirrus-sac that contains a strongly coiled, thick-walled internal sperm duct (vas deferens) and a muscular cirrus of the appearance typical of most proteocephalideans, whereas that of Chambriella is sigmoid, with voluminous, tightly sinuous thick-walled internal sperm duct. In addition, Lenhataenia possesses a well developed internal musculature, whereas the internal musculature of Chambriella is weakly developed, formed by a low number of muscle fibres. The scolex morphology and distribution of microtriches of Peltidocotyle lenha (Woodland, 1933) (syn. Othinoscolex lenha Woodland, 1933 and Othinoscolex myzofer Woodland, 1933), Chambriella sp. and Choanoscolex sp. are described using scanning electron microscopy. The two latter taxa may be new for science and are reported from S. planiceps for the first time.

  8. Morphological observation and length-weight relationship of critically endangered riverine catfish Rita rita (Hamilton).

    Science.gov (United States)

    Amin, M R; Mollah, M F A; Taslima, K; Muhammadullah

    2014-01-15

    The experiment was conducted to investigate the morphological status of the critically endangered riverine catfish Rita rita using morphometric and meristic traits. About 158 species of Rita were collected from the old Brahmaputra river in Mymensingh district and were studied in the laboratory of the Fisheries Biology and Genetics Department, Bangladesh Agricultural University. Measurement of length and weight of Rita were recorded by using measuring scale and electric balance respectively. Significant curvilinear relationship existed between total length and other morphometric characters and between head length and other characters of the head. Relationships between total length and various body measurements of the fish were highly significant (p < 0.01) except the relationship between total length and pelvic fin length of male fish (p < 0.05). In case of meristic characters-dorsal fin rays, pelvic fin rays, pectoral fin rays, anal fin rays, caudal fin rays, number of vertebrae and branchiostegal rays were found to be more or less similar except slight differences. The values of condition factors (k) in the total length body-weight relationships for female and male were found to be 0.41 and 0.38, respectively. The mean values of relative condition factors (kn) were 1.0 and 1.005 for female and male, respectively.

  9. Characterization of dermal plates from armored catfish Pterygoplichthys pardalis reveals sandwich-like nanocomposite structure.

    Science.gov (United States)

    Ebenstein, Donna; Calderon, Carlos; Troncoso, Omar P; Torres, Fernando G

    2015-05-01

    Dermal plates from armored catfish are bony structures that cover their body. In this paper we characterized structural, chemical, and nanomechanical properties of the dermal plates from the Amazonian fish Pterygoplichthys pardalis. Analysis of the morphology of the plates using scanning electron microscopy (SEM) revealed that the dermal plates have a sandwich-like structure composed of an inner porous matrix surrounded by two external dense layers. This is different from the plywood-like laminated structure of elasmoid fish scales but similar to the structure of osteoderms found in the dermal armour of some reptiles and mammals. Chemical analysis performed using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and X-ray diffraction (XRD) results revealed similarities between the composition of P. pardalis plates and the elasmoid fish scales of Arapaima gigas. Reduced moduli of P. pardalis plates measured using nanoindentation were also consistent with reported values for A. gigas scales, but further revealed that the dermal plate is an anisotropic and heterogeneous material, similar to many other fish scales and osteoderms. It is postulated that the sandwich-like structure of the dermal plates provides a lightweight and tough protective layer. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Bioaccumulation of cesium-137 in yellow bullhead catfish (Ameiurus natalis) inhabiting an abandoned nuclear reactor reservoir

    International Nuclear Information System (INIS)

    McCreedy, C.D.; Glickman, L.T.; Jagoe, C.H.; Brisbin, I.L. Jr.

    1997-01-01

    Bioaccumulation of 137 Cs was investigated in yellow bullhead catfish (Ameiurus natalis) inhabiting an abandoned reactor reservoir, Pond B, Savannah River Site, Barnwell Co., South Carolina. The authors collected fish by trap-netting, and determined ages from pectoral spines. Muscle and other tissues were assayed for 137 Cs by NaI-scintillation. Music 137 Cs was unrelated to sex or mass of fish, but was related to age. Examination of least-squares means suggested that 137 Cs in muscle increased up to about age 3, but did not increase with greater age. A modified Richards model showed equilibrium 137 Cs concentration in muscle was acquired in approximately 2.4 years. Growth differed between sexes and the time to asymptotic body mass was longer than the time to attain equilibrium 137 Cs concentration. Males attained an asymptotic mass of 577 g in approximately 6.3 years; females attained an asymptotic mass of 438 g in approximately 5.9 years. The cumulative 137 Cs burden of the population was 4.9 x 10 6 Bq, representing 137 Cs inventory of the reservoir. Concentration of 137 Cs varied among tissues with gill and muscle the lowest and highest. Concentration of 137 Cs in ovaries declined with increasing ovary mass. Until equilibrium is attained in these fish, 137 Cs concentration is directly related to increasing age rather than size

  11. Diel dualism in the energy consumption of the European catfish Silurus glanis.

    Science.gov (United States)

    Slavík, O; Horký, P

    2012-12-01

    Twenty individuals of the largest European freshwater predator, the European catfish Silurus glanis, were tagged with electromyogram (EMG) physiological telemetry sensors. The fish were observed during diel cycles during the spring and summer in the Elbe and Berounka Rivers, Czech Republic. The purpose of this study was to determine whether diel dualism in the activity of S. glanis occurs naturally or is induced by the laboratory environment and by the conditions occurring in aquaculture. The results obtained from the riverine environment tended to show dualism in the use of the light and dark phases of the day because 35% of the individuals varied from a site-specific common diel activity pattern. The EMG values increased in accordance with the mass (M) of the fish. To eliminate the influence of M on individual energy consumption, the EMG records were analysed in terms of the EMG:M ratios. High individual variability was found in these ratios. The diel activity pattern of the individuals with relatively high energy consumption differed from the common diel activity pattern. In contrast, the fish that adopted the common diel activity pattern displayed relatively low energy consumption. The results of this study indicated that dualism and energy consumption are related. The EMG values also varied with the values of the environmental variables. Increasing temperature was associated with high EMG values, whereas the EMG values decreased with increasing flow. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  12. Determinação do sexo em catfish de canal (Ictalurus punctatus utilizando endoscopia

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    Fernando Souza Mendonça De Lima

    2011-06-01

    Full Text Available A identificação precoce do sexo em peixes é um procedimento importante para o desenvolvimento de estratégias que facilitem o manejo no plantel de reprodutores. A endoscopia pode ser utilizada para essa identificação, embora em peixes seu uso ainda seja restrito. Diante disso, o objetivo deste trabalho foi validar o uso do endoscópio rígido via poro urogenital para determinação do sexo do catfish de canal (Ictalurus punctatus. O equipamento utilizado foi um endoscópio Multipurpose Rigid™ 30º com 2,7 mm de diâmetro e 18 cm de comprimento. Foram examinados 60 peixes com peso variando entre 86 e 1.032 gramas nos quais foi introduzido o endoscópio via poro genital e, logo em seguida, eutanaziados para confirmação do sexo. Amostras de tecido gonadal foram coletadas para avaliação histológica do estádio de maturação e confirmação do sexo. Os resultados mostraram que dos 60 peixes examinados, 57 tiveram confirmação positiva do sexo via endoscopia, o que corresponde a 95% da amostra. A utilização da endoscopia para determinação do sexo em I. punctatus via poro genital é viável.

  13. Salinity tolerance of non-native suckermouth armoured catfish (Loricariidae: Pterygoplichthys sp.) from Kerala, India

    Science.gov (United States)

    Kumar, A. Biju; Schofield, Pam; Raj, Smrithy; Satheesh, Sima

    2018-01-01

    Loricariid catfishes of the genus Pterygoplichthys are native to South America and have been introduced in many localities around the world. They are freshwater fishes, but may also use low-salinity habitats such as estuaries for feeding or dispersal. Here we report results of a field survey and salinity-tolerance experiments for a population of Pterygoplichthys sp. collected in Kerala, India. In both chronic and acute salinity-tolerance trials, fish were able to withstand salinities up to 12 ppt with no mortality; however, fish transferred to salinities > 12 ppt did not survive. The experimental results provide evidence that nonnative Pterygoplichthys sp. are able to tolerate mesohaline conditions for extended periods, and can easily invade the brackish water ecosystems of the state. Further, Pterygoplichthys sp. from Kerala have greater salinity tolerance than other congeners. These data are vital to predicting the invasion of non-native fishes such as Pterygoplichthys spp. into coastal systems in Kerala and worldwide. This is particularly important as estuarine ecosystems are under threat of global climate change and sea-level rise. In light of the results of the present study and considering the reports of negative impacts of the species in invaded water bodies, management authorities may consider controlling populations and/or instituting awareness programmes to prevent the spread of this nuisance aquatic invasive species in Kerala.

  14. Regulation and mechanism of leptin on lipid metabolism in ovarian follicle cells from yellow catfish Pelteobagrus fulvidraco.

    Science.gov (United States)

    Zhang, Li-Han; Tan, Xiao-Ying; Wu, Kun; Zhuo, Mei-Qin; Song, Yu-Feng; Chen, Qing-Ling

    2015-10-01

    The present study was conducted to determine the effect of leptin on lipid metabolism in ovarian follicle cells of yellow catfish Pelteobagrus fulvidraco. For that purpose, primary ovarian follicle cells were isolated from yellow catfish, cultured and subjected to different treatments (control, 0.1% DMSO, 500ng/ml leptin, 500ng/ml leptin plus 100μM wortmannin, 500ng/ml leptin plus 50nM AG490, respectively) for 48h. Intracellular triglyceride (TG) content, the activities (CPT I, FAS, G6PD, and 6PGD) and/or expression level of several enzymes (CPT I, FAS, G6PD, 6PGD, ACCa and ACCb), as well as the mRNA expression of transcription factors (PPARα, PPARγ and SREBP-1) involved in lipid metabolism were determined. Recombinant human leptin (rt-hLEP) incubation significantly reduced intracellular TG content, activities and mRNA levels of FAS, G6PD and 6PGD, SREBP-1 and PPARγ, but enhanced activity and mRNA level of CPT I, PPARα and ACCa. Specific inhibitors AG490 and wortmannin of JAK-STAT and IRS-PI3K signaling pathways prevented leptin-induced changes, indicating that JAK-STAT and IRS-PI3K signaling pathways were involved in the process of leptin-induced changes of lipid metabolism. Based on these observations above, for the first time, our study indicated that leptin reduced lipid deposition by activating lipolysis and suppressing lipogenesis in ovarian follicles of yellow catfish, and both JAK-STAT and IRS-PI3K signaling pathways were involved in the changes of leptin-induced lipid metabolism. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Determination of cyanuric acid residues in catfish, trout, tilapia, salmon and shrimp by liquid chromatography-tandem mass spectrometry

    International Nuclear Information System (INIS)

    Karbiwnyk, Christine M.; Andersen, Wendy C.; Turnipseed, Sherri B.; Storey, Joseph M.; Madson, Mark R.; Miller, Keith E.; Gieseker, Charles M.; Miller, Ron A.; Rummel, Nathan G.; Reimschuessel, Renate

    2009-01-01

    In May 2007, investigators discovered that waste material from the pet food manufacturing process contaminated with melamine (MEL) and/or cyanuric acid (CYA) had been added to hog and chicken feeds. At this time, investigators also learned that adulterated wheat gluten had been used in the manufacture of aquaculture feeds. Concern that the contaminated feed had been used in aquaculture and could enter the human food supply prompted the development of a method for the determination of CYA residues in the edible tissues of fish and shrimp. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed as a sensitive technique for the analysis of CYA in catfish, tilapia, salmon, trout and shrimp tissue. CYA was extracted from ground fish or shrimp with an acetic acid solution, defatted with hexane, and isolated with a graphitic carbon black solid-phase extraction column. Residues were separated from matrix components using a porous graphitic carbon LC column, and then analyzed with electrospray ionization in negative ion mode on a triple quadrupole mass spectrometer. Selective reaction monitoring was performed on the [M-H] - m/z 128 ion resulting in the product ions m/z 85 and 42. Recoveries from catfish, tilapia and trout fortified with 10-100 μg kg -1 of CYA averaged 67% with a relative standard deviation (R.S.D.) of 18% (n = 107). The average method detection limit (MDL) for catfish, tilapia and trout is 3.5 μg kg -1 . An internal standard, 13 C 3 -labeled CYA, was used in the salmon and shrimp extractions. Average recovery of CYA from salmon was 91% (R.S.D. = 15%, n = 18) with an MDL of 7.4 μg kg -1 . Average recovery of CYA from shrimp was 85% (R.S.D. = 10%, n = 13) with an MDL of 3.5 μg kg -1

  16. Extraction by Dry Rendering Methode and Characterization Fish Oil of Catfish Viscera Fat by Product of Smooked Fish Processing

    Directory of Open Access Journals (Sweden)

    Kamini Kamini

    2017-02-01

    Full Text Available AbstractThe catfish viscera fat, is cathfish processing by-products, has potential to be used as a source of rawmaterial for production of fish oil. This study aimed to analyze the value of proximate, heavy metal contentand fatty acid profile of catfish viscera fat (Pangasius hypopthalmus and characterized fish oil extracted bydry rendering in various temperature and time than compared it to fish oil extracted by stove heating toobtain the best treatment. Proximate, heavy metal residue, and the fatty acid profile analysis were conductedfor characterizing catfish viscera fat. Fish oil extraction was conducted by dry rendering in varioustemperatures of 50, 60, 70, 80 °C for 1, 2, and 3 hours. Fish oil quality was determined by the chemicalcharacteristics i.e. PV, FFA, anisidin and TOTOX. The results of the study showed that fat content of catfishfat viscera was 88.19 %, the heavy metals content was below SNI standart to be consumed, and fatty acidprofile composition was SFA>MUFA>PUFA. The highest fatty acid content was oleic acid. The best fish oilquality was resulted on temperature extraction of 50°C for 2 hours with yield value, PV, FFA, anisidin, andTOTOX were 45.17 %, 2.77 meq/kg, 0.83 %, 2.86 meq/kg, 8.39 meq/kg respectively. This result was notsignificantly different with fish oil extracted by the stove heating expect for yield and PV were 80.11% and6.52 meq/kg, respectively.

  17. Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824).

    Science.gov (United States)

    Lovatto, Naglezi de Menezes; Goulart, Fernanda Rodrigues; de Freitas, Silvandro Tonetto; Mombach, Patricia Inês; Loureiro, Bruno Bianch; Bender, Ana Betine Beutinger; Boligon, Aline Augusti; Radünz Neto, João; da Silva, Leila Picolli

    2015-12-01

    An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50% of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0.46 g, were distributed into 20 tanks. For data four orthogonal contrasts were applied: control diet versus PSM diets; control diets versus PPCPS diets; control versus other diets; PSM diets versus PPCPS diets. The results indicated that the fish fed PSM diets had lower weight gain when compared to either control diet or PPCPS. The PPCPS do not affect growth and protein efficiency ratio. Lower albumin contents were found for the control diet fish for the contrasts control diet versus PPCPS diet and control diet versus other diets. The hepatic ALAT enzyme activity was higher in the fish fed the control diet (P < 0.05). The hepatic ALP was most active in fish that received the PPCPS diets, when comparing control diet versus PPCPS diets and control diet versus other diets. The hepatosomatic index was higher for fish fed the PPCPS. Our results indicated that PPCPS presents relevant nutritional quality for fish and can replace the fish meal protein up to 50% without affecting growth, PER and intermediate metabolites in silver catfish.

  18. Additional lime of CaO in rearing medium with salinity for growing of catfish Pangasius hypopthalmus

    Directory of Open Access Journals (Sweden)

    Yuni Puji Hastuti

    2013-11-01

    Full Text Available Catfish juvenile quality improvements sought through the addition of lime to the availability of minerals in the body. Media maintenance is set at 4‰ salinity aims to reduce the level of osmotic work to reduce energy used for osmoregulation and can be allocated to the growth of catfish. Juvenile-sized fish used 2.59±0.02 cm and weights 0.13±0.01 g. Aquarium measuring 20×20×20 cm3 used 15 units, filled with water salinity of 4% as 6 L per unit, and added lime CaO appropriate treatment. During the study, the fish were fed with 28% protein content three times a day with FR 5%. Addition of CaO treatment dose 0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L result in the survival rate of 100%, 100%, 100%, 96.29%, and 100% (p>0.05. The growth rate of daily weight 5.196%, 5.212%, 6.596%, 5.35% and 5.101% (p>0.05. Long absolute yield significantly different values are 1.451 cm, 1.518 cm, 1.964 cm, 1.71 cm, and 1.546 cm (p<0.05. During the study, the water quality is within the range that can be tolerated by catfish juvenile. The use of lime in the salinity of 4% is suggested as many as 40 mg/L CaO with the turnover of water every day and feeding enough.Keywords: CaO, salinity, growth, Pangasius hypopthalmus

  19. Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes.

    Science.gov (United States)

    Silva, M A; Costa, G M J; Lacerda, S M S N; Brandão-Dias, P F P; Kalapothakis, E; Silva Júnior, A F; Alvarenga, E R; França, L R

    2016-05-01

    Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n=8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Effects of temperature on sound production and auditory abilities in the Striped Raphael catfish Platydoras armatulus (Family Doradidae.

    Directory of Open Access Journals (Sweden)

    Sandra Papes

    Full Text Available Sound production and hearing sensitivity of ectothermic animals are affected by the ambient temperature. This is the first study investigating the influence of temperature on both sound production and on hearing abilities in a fish species, namely the neotropical Striped Raphael catfish Platydoras armatulus.Doradid catfishes produce stridulation sounds by rubbing the pectoral spines in the shoulder girdle and drumming sounds by an elastic spring mechanism which vibrates the swimbladder. Eight fish were acclimated for at least three weeks to 22°, then to 30° and again to 22°C. Sounds were recorded in distress situations when fish were hand-held. The stridulation sounds became shorter at the higher temperature, whereas pulse number, maximum pulse period and sound pressure level did not change with temperature. The dominant frequency increased when the temperature was raised to 30°C and the minimum pulse period became longer when the temperature decreased again. The fundamental frequency of drumming sounds increased at the higher temperature. Using the auditory evoked potential (AEP recording technique, the hearing thresholds were tested at six different frequencies from 0.1 to 4 kHz. The temporal resolution was determined by analyzing the minimum resolvable click period (0.3-5 ms. The hearing sensitivity was higher at the higher temperature and differences were more pronounced at higher frequencies. In general, latencies of AEPs in response to single clicks became shorter at the higher temperature, whereas temporal resolution in response to double-clicks did not change.These data indicate that sound characteristics as well as hearing abilities are affected by temperatures in fishes. Constraints imposed on hearing sensitivity at different temperatures cannot be compensated even by longer acclimation periods. These changes in sound production and detection suggest that acoustic orientation and communication are affected by temperature changes in

  1. DNA barcode identification of commercial fish sold in Mexican markets.

    Science.gov (United States)

    Sarmiento-Camacho, Stephanie; Valdez-Moreno, Martha

    2018-04-24

    The substitution of high-value fish species for those of lower value is common practice. Although numerous studies have addressed this issue, few have been conducted in Mexico. In this study, we sought to identify fresh fillets of fish, sharks, and rays using DNA barcodes. We analyzed material from "La Viga" in Mexico City, and other markets located on the Gulf and Caribbean coasts of Mexico. From 134 samples, we obtained sequences from 129, identified to 9 orders, 28 families, 38 genera, and 44 species. The most common species were Seriola dumerili, Pangasianodon hypophthalmus, Carcharhinus falciformis, Carcharhinus brevipinna, and Hypanus americanus. Pangasianodon hypophthalmus was most commonly used as a substitute for higher-value species. The substitution rate was 18% of the total. A review of the conservation status of the specimens identified against the IUNC list enabled us to establish that some species marketed in Mexico are threatened: Makaira nigricans, Lachnolaimus maximus, Hyporthodus flavolimbatus, and Isurus oxyrinchus are classified as vulnerable; Lopholatilus chamaeleonticeps and Sphyrna lewini are endangered; and the status of Hyporthodus nigritus is critical. These results will demonstrate to the Mexican authorities that DNA barcoding is a reliable tool for species identification, even when morphological identification is difficult or impossible.

  2. Analysis of eco-innovation with triple helix approach: case-study of biofloc catfish farming in Yogyakarta

    Science.gov (United States)

    Purwadi, D.; Nurlaily, I.

    2018-03-01

    Concerning environmental into focus of innovation process will expand the number of actor involved. Eco-innovation and triple helix are often frameworks applied to analyse how environmental concern are integrated in innovation process and how different stakeholder groups are having inter relation. Case study from biofloc catfish farming in Yogyakarta is presented to demonstrate a possible approach for researching the success of triple helix frameworks. This case is considered on basic of the result of a survey among farmers, academician and government. The paper concludes the creating of full triple helix encounters problem in practice. It also includes suggestion for further research on fisheries development.

  3. Utilization Of The Visceral Organsof Catfish (Pangasius Hypoptalmus) Added To Banana Peel (Musa Acuminata Balbisiana) To Produceliquid Organic Fertilizer

    OpenAIRE

    Fryathama, Ilham; Sukmiwati, Mery; ', Sumarto

    2017-01-01

    This study aimed to obtain liquid organic fertilizer produced from the visceral organs of catfish added to banana skin for enriching the macro elements N, P, and K.The method used in this study was the experimental. Parameters used were the value of pH, nitrogen, phosphorus and potassium. The experimentwas composed as completely randomized design (CRD), and the treatment was addition of banana peel which varied into 4 different amoun, namely: without the banana peel (K0), banana peel 100 g (K...

  4. Neoparaseuratum travasssosi, n. g., n. sp. (Nematoda: Quimperiidae, a new parasite from thorny catfish Pterododas granulosus in Brazil

    Directory of Open Access Journals (Sweden)

    F. Moravec

    1992-01-01

    Full Text Available A new nematode genus and species. Neoparaseuratum travassosi n. g., n. sp., is described from the intestine of the freshwater thorny catfish, Pterodoras granulosus (Valenciennes, from the Paraná River, Brazil. This seuratoid nematode species represents a new genus of the family Quimperiidae, being characterized mainly by the presence of numerous narrow longitudinal bands of inflated cuticle extending along the cephalic region of the body, small deirids, postoesophageal position of the excretory pore, relatively short (0.159-0.303 mm, equal spicules and a gubernaculum, the absence of caudal alae and preanal sucker in the male, and by some other features.

  5. Industrial dry electro-stunning followed by chilling and decapitation as a slaughter method in Claresse (Heteroclarias sp.) and African catfish (Clarius gariepinus)

    NARCIS (Netherlands)

    Sattari, A.; Lambooij, E.; Sharifi, H.; Abbink, W.; Reimert, H.; Vis, van de J.W.

    2010-01-01

    This study evaluated the industrial application of dry electro-stunning for Claresse® and African catfish via monitoring of brain and heart activities, behavioral responses to noxious stimuli and meat quality. Dry electro-stunning (150 V, AC + DC, 100 Hz) of Claresse® for 0.7 ± 0.2 s (mean ± SD)

  6. Enrichment of the African catfish Clarias gariepinus (Burchell) with functional selenium originating from garlic: effect of enrichment period and depuration on total selenium level and sensory properties

    NARCIS (Netherlands)

    Schram, E.; Schelvis-Smit, A.A.M.; Heul, van der J.W.; Luten, J.B.

    2010-01-01

    We wanted to optimize the procedure for the selenium enrichment of farmed African catfish, using garlic as dietary selenium source. In the first experiment we established the relation between the length of the selenium enrichment period and the resulting total selenium level in the fillet of the

  7. The effect of temperature on the mucosal IgM antibody response to DNP-KLH in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    Bath immersion remains a practical route for immunizing against disease in channel catfish; however research efforts in this area have revealed variable results when activating mucosal Ab responses with different antigens. This is likely due to a number of factors including the individual species, ...

  8. Bioaccumulation of Stentorin, the Probable Causative Agent for Discolored ("Purple") Eggs and Ovaries in Blue Catfish (Ictalurus furcatus) from Eufaula Lake, Oklahoma, USA.

    Science.gov (United States)

    Gale, Robert W; Papoulias, Diana M; Schmitt, Christopher J

    2015-08-18

    Observations of reddish to "purple" discolored eggs in the ovaries of adult female blue catfish (Ictalurus furcatus) from the northern arm of Eufaula Lake, a eutrophic multiuse impoundment in east-central Oklahoma, were first reported in 2006. Blue catfish eggs are normally cream to light yellow. Reports peaked in 2007-2008 and declined through 2009-2010; purple eggs have not been reported between 2010 and 2014. In the laboratory, all tissues and fluids of affected fish were strongly orange-red fluorescent under UV illumination, with the fluorescence most apparent in the lipid-rich ovaries and eggs. The causative agent was isolated chromatographically and confirmed by mass spectrometry as stentorin (1,3,4,6,8,10,11,13-octahydroxy-2,5-diisopropyl-phenanthro[1,10,9,8,o,p,q,r,a]perylene-7,14-dione), the fluorescent, lipophilic pigment associated with the photoreceptor protein of the ciliated protozoan Stentor coeruleus (Heterotrichea; Stentoridae). Larval medaka (Orizias latipes) readily consumed S. coeruleus in the laboratory and were observed to fluoresce in the same manner as the affected blue catfish. Potential deleterious effects of stentorin bioaccumulation remain to be determined, as do the geographic extent and the identities of other fluorescent compounds isolated from catfish eggs and ovaries.

  9. Bioaccumulation of stentorin, the probable causative agent for discolored (“purple”) eggs and ovaries in blue catfish (Ictalurus furcatus) from Eufaula Lake, Oklahoma, USA

    Science.gov (United States)

    Gale, Robert W.; Papoulias, Diana M.; Schmitt, Christopher J.

    2015-01-01

    Observations of reddish to “purple” discolored eggs in the ovaries of adult female blue catfish (Ictalurus furcatus) from the northern arm of Eufaula Lake, a eutrophic multiuse impoundment in east-central Oklahoma, were first reported in 2006. Blue catfish eggs are normally cream to light yellow. Reports peaked in 2007–2008 and declined through 2009–2010; purple eggs have not been reported between 2010 and 2014. In the laboratory, all tissues and fluids of affected fish were strongly orange-red fluorescent under UV illumination, with the fluorescence most apparent in the lipid-rich ovaries and eggs. The causative agent was isolated chromatographically and confirmed by mass spectrometry as stentorin (1,3,4,6,8,10,11,13-octahydroxy-2,5-diisopropyl-phenanthro[1,10,9,8,o,p,q,r,a]perylene-7,14-dione), the fluorescent, lipophilic pigment associated with the photoreceptor protein of the ciliated protozoan Stentor coeruleus (Heterotrichea; Stentoridae). Larval medaka (Orizias latipes) readily consumed S. coeruleus in the laboratory and were observed to fluoresce in the same manner as the affected blue catfish. Potential deleterious effects of stentorin bioaccumulation remain to be determined, as do the geographic extent and the identities of other fluorescent compounds isolated from catfish eggs and ovaries.

  10. Changes in fatty acid concentrations in tissues of African catfish, Clarias gariepinus, as a consequence of dietary carnitine, lysine and lipid supplements

    NARCIS (Netherlands)

    Ozorio, E.O.A.; Uktolseja, J.L.A.; Huisman, E.A.; Verreth, J.A.J.

    2001-01-01

    A study was undertaken to examine the effect of different dietary carnitine (200 and 1000 mg/kg diet) and fat (90 and 190 g/kg diet) supplementation on growth and fatty acid concentrations of fish fed either with a low- (13 g/kg) or a high-lysine (21 g/kg) diet. African catfish (22?7 g/fish),

  11. THE PREVALENCE OF Salmonella sp., Listeria sp. AND Aeromonas spp. IN CATFISH (CLARIAS (Clarias gariepinus AND TILAPIA (Tilapia mossambica BY PELLETING METHOD

    Directory of Open Access Journals (Sweden)

    Titik Budiati

    2013-06-01

    Full Text Available The aim of this study was to modify the isolation methods of Aeromonas sp., Salmonella spp., and Listeria sp. in catfish (Clarias gariepinus and tilapia (Tilapia mossambica obtained from wet markets and ponds in Malaysia by pelleting the sample. A total of 108 samples (32 catfish intestines, 32 tilapia intestines, and 44 water samples were obtained from nine wet markets and eight ponds. The modified method was employed by pelleting the samples and followed by either implementing pre-enrichment or without pre-enrichment on the isolation of Salmonella and Listeria spp. The modified method (by pelleting the sample in combination with pre-enrichment was the most efficient for Salmonella and Listeria isolation. The sensitivity of the modified Salmonella isolation method was 0.53 and 0.73 for fish and water samples, respectively. The sensitivity of the modified Listeria method was 1 and 0.92 for fish and water samples, respectively. However, the sensitivity of the method by pelleting the sample was similar to those of non-pelleting the sample on Aeromonas isolation. Five species of Aeromonas spp., seven serovars of Salmonella sp., and four species of Listeria sp. were observed in catfish, tilapia and water samples. Overall, by pelleting the sample offered the beneficial to isolate Aeromonas spp., Salmonella sp. and Listeria spp. in catfish, tilapia and water.

  12. Evaluation of the therapeutic effect of potassium permanganate at early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    The efficacy of potassium permanganate (KMnO4) against early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus) was evaluated. Fish were experimentally challenged, by waterborne exposure for 2 h to F. columnare after cutaneous abrasion, an...

  13. Isoenergetic Replacement of Fat by Starch in Diets for African Catfish (Clarias gariepinus): Effect on Water Fluxes in the Gastro Intestinal Tract

    NARCIS (Netherlands)

    Harter, T.S.; Verreth, J.A.J.; Heinsbroek, L.T.N.; Schrama, J.W.

    2013-01-01

    The effect of an isoenergetic replacement of dietary fat by starch, on chyme characteristics and water fluxes in the gastro intestinal tract (GIT) was assessed. Adult African catfish (Clarias gariepinus) were fed a starch (SD) or fat (FD) diet and groups of fish were dissected at 2, 5 and 8 h after

  14. Effect of Pre-cooking and Addition of Phosphate on the Quality of Catfish Fillets Cooked in Pouch in Boiling Water

    Science.gov (United States)

    Cooking or reheating food in a vacuum sealed bag has been a common method of preparing vegetables, meat and poultry products. There are very few examples of vacuum sealed bags designed for cooking or reheating catfish fillets. The objective of the present study was to examine the properties of raw f...

  15. Effects of feeding frequency on apparent energy and nutrient digestibility/availability of channel catfish, Ictalurus punctatus, reared at optimal and suboptimal temperatures

    Science.gov (United States)

    This study examined the effects of feeding frequency (daily versus every other day [EOD]) on nutrient digestibility/availability of channel catfish, Ictalurus punctatus, reared at optimal (30 C) and suboptimal (24 C) temperatures. A 28% protein practical diet was used as the test diet, and chromic o...

  16. Effects of exercise on l-carnitine and lipid metabolism in African catfish (Clarias gariepinus) fed different dietary l-carnitine and lipid levels

    NARCIS (Netherlands)

    Ozorio, R.O.A.; Ginneken, van V.J.T.; Bessa, R.J.B.; Verstegen, M.W.A.; Verreth, J.A.J.; Huisman, E.A.

    2010-01-01

    African catfish (Clarias gariepinus) were fed four isonitrogenous diets (34 % crude protein), each containing one of two lipid (100 or 180 g/kg) and two l-carnitine (15 or 1000 mg/kg) levels. After 81 d of feeding, thirty-two fish (body weight 32 g) from each dietary group were randomly selected,

  17. Development of phytoplankton communities and common off-flavors in a biofloc technology system used to culture channel catfish Ictalurus punctatus

    Science.gov (United States)

    The use of biofloc technology production systems continues to increase in the aquaculture industry worldwide. Recent research demonstrated that outdoor biofloc systems can be used to produce high yields of channel catfish (Ictalurus punctatus). However, studies have not yet been performed to deter...

  18. Development of phytoplankton communities and common off-flavors in a biofloc technology system used for the culture of channel catfish (Ictalurus punctatus)

    Science.gov (United States)

    The use of biofloc technology production systems continues to increase in the aquaculture industry worldwide. Recent research demonstrated that outdoor biofloc systems can be used to produce high yields of channel catfish (Ictalurus punctatus). However, studies have not yet been performed to deter...

  19. New records of the mailed catfish Planiloricaria cryptodon from the upper Amazon in Peru, Brazil and Bolivia, with a key to the genera of the Planiloricariina

    NARCIS (Netherlands)

    Isbrücker, I.J.H.; Nijssen, H.

    1986-01-01

    The mailed catfish Planiloricaria cryptodon (Isbrücker, 1971) was hitherto only known from the holotype from Río Ucayali, Peru. New material from Rio Purus (Est. Acre, Brazil) and from Río Mamoré (Prov. Beni, Bolivia) is described and compared with the holotype. One of the new specimens is

  20. Life cycle assessment of intensive striped catfish farming in the Mekong Delta for screening hotspots as input to environmental policy and research agenda

    NARCIS (Netherlands)

    Bosma, R.H.; Pham Thi Ahn,; Potting, J.

    2011-01-01

    Purpose Intensive striped catfish production in the Mekong Delta has, in recent years, raised environmental concerns. We conducted a stakeholder-based screening life cycle assessment (LCA) of the intensive farming system to determine the critical environmental impact and their causative processes in

  1. Salt Sensitive Tet-Off-Like Systems to Knockdown Primordial Germ Cell Genes for Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus.

    Science.gov (United States)

    Li, Hanbo; Su, Baofeng; Qin, Guyu; Ye, Zhi; Alsaqufi, Ahmed; Perera, Dayan A; Shang, Mei; Odin, Ramjie; Vo, Khoi; Drescher, David; Robinson, Dalton; Zhang, Dan; Abass, Nermeen; Dunham, Rex A

    2017-05-31

    Repressible knockdown approaches were investigated for transgenic sterilization in channel catfish, Ictalurus punctatus . Two primordial germ cell (PGC) marker genes, nanos and dead end , were targeted for knockdown, and an off-target gene, vasa , was monitored. Two potentially salt sensitive repressible promoters, zebrafish adenylosuccinate synthase 2 (ADSS) and zebrafish racemase (Rm), were each coupled with four knockdown strategies: ds-sh RNA targeting the 5' end (N1) or 3' end (N2) of channel catfish nanos , full-length cDNA sequence of channel catfish nanos for overexpression (cDNA) and ds-sh RNA targeting channel catfish dead end (DND). Each construct had an untreated group and treated group with sodium chloride as the repressor compound. Spawning rates of full-sibling P₁ fish exposed or not exposed to the constructs as treated and untreated embryos were 93% and 59%, respectively, indicating potential sterilization of fish and repression of the constructs. Although the mRNA expression data of PGC marker genes were inconsistent in P₁ fish, most F₁ individuals were able to downregulate the target genes in untreated groups and repress the knockdown process in treated groups. The results indicate that repressible transgenic sterilization is feasible for reproductive control of fish, but more data from F₂ or F₃ are needed for evaluation.

  2. Salt Sensitive Tet-Off-Like Systems to Knockdown Primordial Germ Cell Genes for Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus

    Directory of Open Access Journals (Sweden)

    Hanbo Li

    2017-05-01

    Full Text Available Repressible knockdown approaches were investigated for transgenic sterilization in channel catfish, Ictalurus punctatus. Two primordial germ cell (PGC marker genes, nanos and dead end, were targeted for knockdown, and an off-target gene, vasa, was monitored. Two potentially salt sensitive repressible promoters, zebrafish adenylosuccinate synthase 2 (ADSS and zebrafish racemase (Rm, were each coupled with four knockdown strategies: ds-sh RNA targeting the 5′ end (N1 or 3′ end (N2 of channel catfish nanos, full-length cDNA sequence of channel catfish nanos for overexpression (cDNA and ds-sh RNA targeting channel catfish dead end (DND. Each construct had an untreated group and treated group with sodium chloride as the repressor compound. Spawning rates of full-sibling P1 fish exposed or not exposed to the constructs as treated and untreated embryos were 93% and 59%, respectively, indicating potential sterilization of fish and repression of the constructs. Although the mRNA expression data of PGC marker genes were inconsistent in P1 fish, most F1 individuals were able to downregulate the target genes in untreated groups and repress the knockdown process in treated groups. The results indicate that repressible transgenic sterilization is feasible for reproductive control of fish, but more data from F2 or F3 are needed for evaluation.

  3. Assessment of electrical stunning in fresh water of African Catfish (Clarias gariepinus) and chilling in ice water for loss of consciousness and sensibility

    NARCIS (Netherlands)

    Lambooij, E.; Kloosterboer, R.J.; Gerritzen, M.A.; Vis, van de J.W.

    2006-01-01

    The overall objective of the study was to evaluate loss of consciousness and sensibility after electrical stunning in fresh water and live chilling in ice water for slaughter of African catfish using measurement of electrical brain and heart activity. To provoke immediate loss of consciousness and

  4. COMPARISON OF POND AND RACEWAY PRODUCTION METHODS ON TEXTURE OF CHANNEL CATFISH (Ictalurus punctatus) FILLETS, SHOWING A DEPENDENCY ON SIZE AND FILLET POSITION

    Science.gov (United States)

    The objective of this study was to compare the effect of the production environment (pond vs in-pond raceway) on the chemical composition, color, and textural properties of channel catfish fillets. Compositional analysis consisted of percent moisture, lipid, protein, and ash content. Additional sa...

  5. Increased immunoglobulin production in silver catfish (Rhamdia quelen exposed to agrichemicals

    Directory of Open Access Journals (Sweden)

    L.C. Kreutz

    2014-06-01

    Full Text Available Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.4° or 21.3±0.3°C. The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h on specific serum antibodies were also investigated. Antibodies to A. hydrophila were detected as early as 7 days post-inoculation and increased steadily up to 35 days. The kinetics of antibody production were similar in fish kept at 17.4±0.4° and 21.3±0.3°C, and reinoculation of antigen at 21 days after priming failed to increase specific antibody levels. Intriguingly, we found that, in fish exposed to atrazine and glyphosate, the secretion of specific antibodies was higher than in non-exposed inoculated fish. These findings are important for the design of vaccines and vaccination strategies in Neotropical fish species. However, because atrazine and glyphosate are widespread contaminants of soil and water, their immune-stimulating effect could be harmful, in that fish living in herbicide-contaminated water might have increased concentrations of nonspecific antibodies that could mediate tissue injury.

  6. Lower than expected mercury concentration in piscivorous African sharptooth catfish Clarias gariepinus (Burchell)

    International Nuclear Information System (INIS)

    Desta, Zerihun; Borgstrom, Reidar; Rosseland, Bjorn Olav; Dadebo, Elias

    2007-01-01

    The concentrations of total mercury (THg), stable isotopes of nitrogen (δ 15 N) and carbon (δ 13 C), and the diet of the African sharptooth catfish Clarias gariepinus in Lake Awassa, Ethiopia, were studied from January 2003 to February 2004. Values of the δ 15 N were used as an index of trophic position in four length groups and compared to actual stomach contents. The diet of C. gariepinus within the length range of 201-600 mm L T mainly consisted of the small barb Barbus paludinosus, aquatic insects, and mollusks. The proportion of fish prey in the diet was 60% by volume, irrespective of fish size. The differences in δ 15 N values of individuals within and between length classes were less than 3 per mille , and were not significantly related to total length, showing the similarity in trophic niche of the different sizes, which corresponded to recorded stomach contents. Mercury concentrations were in the range of 0.002-0.154 mg kg -1 ww, and had no significant relationship to total length. Hence, even large specimens of C. gariepinus have Hg values below the WHO threshold of 0.2 mg kg -1 ww. The slope of the regression line between log [Hg] and δ 15 N was small, 0.06, indicating the absence of trophic shifts and biomagnification of Hg in larger specimens in our samples. The low Hg concentrations in C. gariepinus compared to the Hg concentrations in other piscivorous fish species in Lake Awassa, such as Barbus intermedius and B. paludinosus, may be due to its dependence on invertebrate preys at small size, diet switching towards low Hg prey fish at larger size, and growth biodilution owing to higher growth rate

  7. Retention and removal 137Cs in Japanese catfish (Silurus asotus Linnaeus) in dressing and cooking method

    International Nuclear Information System (INIS)

    Malek, M.A.; Nakahara, N.; Nakamura, R.

    2004-01-01

    Japanese catfish contaminated by 137 Cs have been used to investigate how the dressing and cook-ing method affect the removal of radioactivity from the fish. During the dressing, 6.0% of the initial 137 Cs activity was removed by washing the live fish, further 30.3% of the activity relative to washed fish was removed by discarding the non-edible body parts (skeletons, fins, visceral mass, liver, kidney etc.) and washing the chopped fish. Fish curry was cooked using various spices, green-stuffs and vegetable oil following a method commonly used in South East and East Asian countries. The cooking process removed a further 61.6% of the 137 Cs activity relative to the activity in dressed fish. Taken together, this normal domestic fish dressing and culinary process removed 74.7% of the initial 137 Cs activity present in the live fish. During the cook-ing, the radioactivity removed from the fish pieces was found to distributed over the ingredients of the curry. The cooked fish pieces retained an average, 38.5% of the radioactivity present in the dressed raw fish pieces. Among the ingredients, the gravy was found to contain an average of 34.8% of the activity of the dressed fish. The activity in green-stuffs was found to vary from 4.0% (in cauliflower) to 7.2% (in potato). It may be concluded that normal home preparation and culinary processes removed the radio-activity from the fish to a great extent. In addition, discarding the gravy and green-stuffs of the curry further reduce the radioactivity intake into the human body. (author)

  8. Uptake, retention and organ/tissue distribution of 137Cs by Japanese catfish (Silurus asotus Linnaeus)

    International Nuclear Information System (INIS)

    Malek, M.A.; Nakahara, M.; Nakamura, R.

    2004-01-01

    The work describes the uptake, retention/biological elimination and organ/tissue distribution of 137 Cs by freshwater Japanese catfish (Silurus asotus Linnaeus) under laboratory conditions. The fish were divided into three groups based on their size and age and reared in 137 Cs-spiked water. The concentration of 137 Cs in the whole body of the live fish was measured at regular intervals up to 60 days. A significant accumulation of 137 Cs was found, but a steady state condition was not achieved by the end of the experiment. The bioaccumulation factors at steady state and the required time to reach steady state were estimated to be 1.55 and 255 days, 1.76 and 180 days and 1.99 and 160 days for large, medium and small size fish, respectively. To determine the effective half-life of 137 Cs, the fish were transferred and reared in the non-contaminated host water. The concentration of the remaining 137 Cs in the whole body of the live fish was measured up to 66 days. The average effective half-life of 137 Cs in the fish species was found to be ∼142 days for fish of all sizes. The distribution of 137 Cs in different organs/tissues of the fish was determined. Accumulation of 137 Cs in muscle/flesh of the fish was found to be ∼75% of whole body accumulation. The uptake rate and the retention capability of juvenile fish were found to be higher and therefore, these were more susceptible to 137 Cs than adult and old fish, and could be an important source of 137 Cs in the human food chain

  9. Trophic diversity in the evolution and community assembly of loricariid catfishes

    Directory of Open Access Journals (Sweden)

    Lujan Nathan K

    2012-07-01

    Full Text Available Abstract Background The Neotropical catfish family Loricariidae contains over 830 species that display extraordinary variation in jaw morphologies but nonetheless reveal little interspecific variation from a generalized diet of detritus and algae. To investigate this paradox, we collected δ13C and δ15N stable isotope signatures from 649 specimens representing 32 loricariid genera and 82 species from 19 local assemblages distributed across South America. We calculated vectors representing the distance and direction of each specimen relative to the δ15N/δ13C centroid for its local assemblage, and then examined the evolutionary diversification of loricariids across assemblage isotope niche space by regressing the mean vector for each genus in each assemblage onto a phylogeny reconstructed from osteological characters. Results Loricariids displayed a total range of δ15N assemblage centroid deviation spanning 4.9‰, which is within the tissue–diet discrimination range known for Loricariidae, indicating that they feed at a similar trophic level and that δ15N largely reflects differences in their dietary protein content. Total range of δ13C deviation spanned 7.4‰, which is less than the minimum range reported for neotropical river fish communities, suggesting that loricariids selectively assimilate a restricted subset of the full basal resource spectrum available to fishes. Phylogenetic regression of assemblage centroid-standardized vectors for δ15N and δ13C revealed that loricariid genera with allopatric distributions in disjunct river basins partition basal resources in an evolutionarily conserved manner concordant with patterns of jaw morphological specialization and with evolutionary diversification via ecological radiation. Conclusions Trophic partitioning along elemental/nutritional gradients may provide an important mechanism of dietary segregation and evolutionary diversification among loricariids and perhaps other taxonomic

  10. Ionoregulatory Impairment in African Catfish (Clarias gariepinus Exposed to Combined Stressors of Lead and Cypermethrin

    Directory of Open Access Journals (Sweden)

    Omowumi O ADEWALE

    2014-03-01

    Full Text Available The contamination of aquatic ecosystem with heavy metals and pesticides has increased dramatically over the years, and this has generated a lot of attention by environmental experts. There are reports on impacts of different single toxicants on aquatic organisms but not much has been done to investigate the impacts of mixtures of toxicants on aquatic life. In this study we investigated the effects of multiple stressors of Pb and cypermethrin on ionoregulation in the African catfish (Clarias gariepinus. Adults C. gariepinus were exposed to either borehole water (control, 2 mg/L Pb, 0.5 μg/L cypermetrin or a mixture of 2 mg/L Pb and 0.5 μg/L cypermethrin for 96 h, after which the levels of plasma Na+, Cl- and K+ were determined as a measure of branchial ionoregulatory disturbance. The exposure of C. gariepinus to 0.5 μg/L cypermethrin resulted in a significant reduction in plasma Na+ (43% decrease and Cl- (31% decrease but a 2-fold increase in plasma K+ concentrations. Exposure to a mixture of cypermethrin and Pb resulted in 53% decrease in plasma Na+, 40% decrease in plasma Cl- and a 3- fold increase in plasma K+. There was no significant difference between the control and those exposed to just only 2 mg/L Pb for all the parameters measured. The results of this study indicated that exposure of aquatic organisms to combined stressors of heavy metals pollution as well as pesticide contamination could have a serious effect on the physiology especially on ionic regulation in the exposed fish.

  11. Multilocus molecular phylogeny of the suckermouth armored catfishes (Siluriformes: Loricariidae) with a focus on subfamily Hypostominae.

    Science.gov (United States)

    Lujan, Nathan K; Armbruster, Jonathan W; Lovejoy, Nathan R; López-Fernández, Hernán

    2015-01-01

    The Neotropical catfish family Loricariidae is the fifth most species-rich vertebrate family on Earth, with over 800 valid species. The Hypostominae is its most species-rich, geographically widespread, and ecomorphologically diverse subfamily. Here, we provide a comprehensive molecular phylogenetic reappraisal of genus-level relationships in the Hypostominae based on our sequencing and analysis of two mitochondrial and three nuclear loci (4293bp total). Our most striking large-scale systematic discovery was that the tribe Hypostomini, which has traditionally been recognized as sister to tribe Ancistrini based on morphological data, was nested within Ancistrini. This required recognition of seven additional tribe-level clades: the Chaetostoma Clade, the Pseudancistrus Clade, the Lithoxus Clade, the 'Pseudancistrus' Clade, the Acanthicus Clade, the Hemiancistrus Clade, and the Peckoltia Clade. Results of our analysis, which included type- and non-type species for every valid genus in Hypostominae, support the reevaluation and restriction of several historically problematic genera, including Baryancistrus, Cordylancistrus, Hemiancistrus, and Peckoltia. Much of the deep lineage diversity in Hypostominae is restricted to Guiana Shield and northern Andean drainages, with three tribe-level clades still largely restricted to the Guiana Shield. Of the six geographically widespread clades, a paraphyletic assemblage of three contain lineages restricted to drainages west of the Andes Mountains, suggesting that early diversification of the Hypostominae predated the late Miocene surge in Andean uplift. Our results also highlight examples of trophic ecological diversification and convergence in the Loricariidae, including support for three independent origins of highly similar and globally unique morphological specializations for eating wood. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. CATFISH KECIL UNIK, Corydoras sp. UNTUK AKUARIUM, TINGKAH LAKU BIOLOGI DAN REPRODUKSINYA

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    Darti Satyani

    2008-12-01

    Full Text Available Corydoras sp. termasuk dalam familia Collichthyidae, kelas Siluridae dan sangat dikenal olah para hobiis ikan hias air tawar. Genus Corydoras yang berasal dari Amerika Selatan ini mempunyai banyak spesies tetapi yang banyak beredar dan sudah dibudidayakan ada 10 spesies yaitu C. aeneus, C. adolfoi, C. barbatus, C. paleatus, C. panda, C. pygmaeus, C. rabauti, C. septentrionalis, C. sterbai, dan C. sychri. Selain ukurannya yang umumnya kecil (maksimum 7,5 cm dibandingkan dengan jenis catfish lain, jenis ini mempunyai dua baris sisik keras. Bentuk badannya kompak agak pipih ke samping dengan mulut menghadap ke bawah. Hidup merayap di dasar pada suhu 24°C--28°C (tergantung spesiesnya; pH 7,0--7,5; dan hardness sekitar 10° dH. Disebut “tukang bersih-bersih” karena senang membersihkan dinding akuarium dengan mulutnya. Tingkah laku reproduksinya amat unik. Sebelum ovulasi induk betina akan menempatkan mulutnya kearah genital induk jantan yang dikenal dengan “posisi T” dan akan mengisap spermanya. Sperma ini akan dilepas melewati usus bersama dengan lepasnya telur kedalam “kantong” yang dibentuk oleh kedua sirip perutnya. Pembuahan efektif terjadi di sini. Kemudian telur akan dilekatkan ke substrat atau objek (daun, batu datar, dan sebagainya yang sebelumnya telah dibersihkan oleh induk jantannya. Telur yang ditinggalkan akan menetas di substrat bila kondisi airnya sesuai dan cukup baik.

  13. Removal of 137Cs in Japanese catfish during preparation for consumption

    International Nuclear Information System (INIS)

    Malek, M.A.; Nakahara, M.; Nakamura, R.

    2004-01-01

    Japanese catfish contaminated by 137 Cs have been used to investigate how dressing and cooking methods affect the removal of radioactivity from the fish. During the dressing, 6.0% of the initial 137 Cs activity in live fish was removed by washing them, and a further 30.3% of this activity relative to the washed fish was removed by discarding the nonedible body parts (such as the skeleton, fins, visceral mass, liver, and kidney) and by washing the chopped edible remains. Fish curry was cooked with various spices, vegetable oil, and greens and other vegetables following a method commonly used in Southeast Asian and East Asian countries. The cooking process removed a further 61.6% of the 137 Cs activity relative to the activity in dressed fish. Taken together, this normal domestic fish dressing and culinary process removed 74.7% of the initial 137 Cs activity that had been present in the live fish. During the cooking, the radioactivity removed from the fish pieces was found to be distributed throughout the ingredients of the curry. The cooked pieces retained, on average, 38.5% of the radioactivity present in the raw dressed pieces. Among the ingredients, the gravy was found to contain an average of 34.8% of the activity of the dressed fish. The activity in greens and vegetables was found to vary from 4.0% (in cauliflower) to 7.2% (in potatoes). It may be concluded that normal home preparation and culinary processes removed much of the radioactivity from the fish. (author)

  14. Survival and growth of catfish Pangasionodon sp. larvae fed on vitamin C-enriched Artemia

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    Mia Setiawati

    2015-05-01

    Full Text Available ABSTRACT The research was conducted to evaluate effect of the administration of Artemia enriched with vitamin C on the survival, total body length, and average body weight of Pangasionodon sp. larvae. Artemia were enriched with either 0, 50, 100, or 150 mg/L vitamin C immersed in the culture medium for 13 hours. Two days old larvae were fed on enriched Artemia 12 times/day for seven days. At the end of feeding experiment, it was found that fish fed on Artemia enriched with 100 mg/L culture medium had the highest survival, total length and body weight, respectively 76.17±12.78%, 1.63±0.34 cm, and 20.6±3.8 mg. Keywords: survival, growth, Artemia, vitamin C, catfish, Pangasionodon sp.  ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh Artemia yang diperkaya dengan vitamin C dosis berbeda terhadap kelangsungan hidup, panjang total, dan bobot tubuh larva ikan patin Pangasionodon sp. Pada penelitian ini, Artemia diperkaya dengan vitamin C dosis 0, 50, 100, atau 150 mg/L media pengayaan. Larva yang berumur dua hari diberi pakan Artemia hasil pengayaan sebanyak 12 kali sehari selama tujuh hari masa pemeliharaan. Pada akhir percobaan, didapatkan bahwa larva ikan yang diberi Artemia diperkaya vitamin C dosis 100 mg/L media pengayaan memberikan kelangsungan hidup 76,17±12,78%, panjang total 1,63±0,34 cm, dan bobot tubuh paling tinggi 20,6±3,8 mg. Kata kunci: kelangsungan hidup, pertumbuhan, Artemia, vitamin C, ikan patin, Pangasionodon sp. 

  15. Molecular cloning, expression analysis and transcript localization of testicular orphan nuclear receptor 2 in the male catfish, Clarias batrachus.

    Science.gov (United States)

    Murugananthkumar, R; Akhila, M V; Rajakumar, A; Mamta, S K; Sudhakumari, C C; Senthilkumaran, B

    2016-12-01

    Testicular receptor 2 (TR2; also known as Nr2c1) is one of the first orphan nuclear receptors identified and known to regulate various physiological process with or without any ligand. In this study, we report the cloning of full length nr2c1 and its expression analysis during gonadal development, seasonal testicular cycle and after human chorionic gonadotropin (hCG) induction. In addition, in situ hybridization (ISH) was performed to localize nr2c1 transcripts in adult testis and whole catfish (1day post hatch). Tissue distribution and gonadal ontogeny studies revealed high expression of nr2c1 in developing and adult testis. Early embryonic stage-wise expression of nr2c1 seems to emphasize its importance in cellular differentiation and development. Substantial expression of nr2c1 during pre-spawning phase and localization of nr2c1 transcripts in sperm/spermatids were observed. Significant upregulation after hCG induction indicate that nr2c1 is under the regulation of gonadotropins. Whole mount ISH analysis displayed nr2c1 expression in notochord indicating its role in normal vertebrate development. Taken together, our findings suggest that nr2c1 may have a plausible role in the testicular and embryonic development of catfish. Copyright © 2015. Published by Elsevier Inc.

  16. Dorsal light reflex is absent in the postural control system of the upside-down swimming catfish, Synodontis nigriventris

    Science.gov (United States)

    Ohnishi, T.; Ohnishi, K.; Okamoto, N.; Yamamoto, T.; Hosoi, H.; Takahashi, A.; Kawai, H.

    A kind of catfish, Synodontis nigriventris, has a unique habit of maintaining an upside-down posture under normal gravity conditions (1 G). We exposed S. nigriventris to a microgravity environment provided by the parabolic flights of an aircraft and observed the dorsal light reflex (DLR), which is well known to be an important visually guided postural reaction in fish. In general, fish directs its back to an illuminated direction, depending on DLR: DLR is observed more clearly under microgravity as compared with 1 G. Interestingly, S. nigriventris exhibited no DLR response even under microgravity. In contrast, clear DLR was observed under microgravity in two other species, which have an upside-up swimming habit, Synodontis multipunctatus, belonging to the same Synodontis family, and Corydoras paleatus, belonging to a different catfish family. Our parabolic flight experiments have confirmed for the first time that S. nigriventris has a novel balance sensation which does not induce DLR. This allows us to address a new and attractive strategy for the analysis of the postural control mechanism of vertebrate.

  17. Growth and contribution of stocked channel catfish, Ictalurus punctatus (Rafinesque, 1818): the importance of measuring post-stocking performance

    Science.gov (United States)

    Stewart, David R.; Long, James M.

    2015-01-01

    In this study it was sought to quantify post-stocking growth, survival, and contribution of advanced size (178 mm total length [TL]) channel catfish Ictalurus punctatus fingerlings, something rarely done. Channel catfish populations were evaluated before (May 2010) and after (May to August 2011 and 2012) stocking. Relative abundance, stocking contribution, and growth were different (P stocked in Lake Lone Chimney, stocking contribution was lower (3–35%), and average length and weight of stocked fish by age-2 reached 230 mm TL and 85 g, whereas the stocking contribution (84–98%) and growth in length (340 mm TL) and weight (280 g) were higher by age-2 in Lake Greenleaf. Given these unambiguous differences of post-stocking performance, benchmark metrics that represent population-level information such as relative abundance and average length and weight of the sample masked these significant differences, highlighting the importance of marking hatchery-fish and then following them through time to determine the effectiveness of stocking. These results suggest that stock enhancement programmes would benefit from studies that quantify post-stocking performance of hatchery fish.

  18. Effects of diets containing vegetable protein concentrates on performance and activity of digestive enzymes in silver catfish (Rhamdia quelen

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    Naglezi de Menezes Lovatto

    2014-02-01

    Full Text Available The purpose of study was to evaluate the effect of using protein concentrates crambe and sunflower meal in the diet of silver catfish juveniles, as substitute for animal protein source. A total of 300 silver catfish had been separate in 15 experimental units of 280 L, totaling five treatments with three replications. We evaluated two levels (25% and 50% replacement of the meat and bone meal by protein concentrates of crambe and sunflower meals. Evaluated growth parameters, biological index and digestive enzymes in fish. There was no statistical difference for mass (g and standard length (cm, but the fish diet CPFCr-25% had greater total length (cm. No difference in dry matter, crude protein and total protein deposited (calculated. However, there was a higher concentration of ash in the carcass of the animals fed the control diet and CPFCr-50% in relation to diet CPFG- 50%, in addition, higher levels of lipids in fish fed diet CPFG-50%. No significant differences for hepatosomatic index, digestive somatic index and intestinal quotient of animals subjected to different treatments. The activity of digestive enzymes trypsin and chymotrypsin did not change. There was increased activity of acid protease. The quantitative and qualitative increase in protein concentration from this fraction allows the use of bran protein concentrates crambe and sunflower as substitutes for animal protein source.

  19. Aeromonas caviae alters the cytosolic and mitochondrial creatine kinase activities in experimentally infected silver catfish: Impairment on renal bioenergetics.

    Science.gov (United States)

    Baldissera, Matheus D; Souza, Carine F; Júnior, Guerino B; Verdi, Camila Marina; Moreira, Karen L S; da Rocha, Maria Izabel U M; da Veiga, Marcelo L; Santos, Roberto C V; Vizzotto, Bruno S; Baldisserotto, Bernardo

    2017-09-01

    Cytosolic and mitochondrial creatine kinases (CK), through the creatine kinase-phosphocreatine (CK/PCr) system, provide a temporal and spatial energy buffer to maintain cellular energy homeostasis. However, the effects of bacterial infections on the kidney remain poorly understood and are limited only to histopathological analyses. Thus, the aim of this study was to investigate the involvement of cytosolic and mitochondrial CK activities in renal energetic homeostasis in silver catfish experimentally infected with Aeromonas caviae. Cytosolic CK activity decreased in infected animals, while mitochondrial CK activity increased compared to uninfected animals. Moreover, the activity of the sodium-potassium pump (Na + , K + -ATPase) decreased in infected animals compared to uninfected animals. Based on this evidence, it can be concluded that the inhibition of cytosolic CK activity by A. caviae causes an impairment on renal energy homeostasis through the depletion of adenosine triphosphate (ATP) levels. This contributes to the inhibition of Na + , K + -ATPase activity, although the mitochondrial CK activity acted in an attempt to restore the cytosolic ATP levels through a feedback mechanism. In summary, A. caviae infection causes a severe energetic imbalance in infected silver catfish, which may contribute to disease pathogenesis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Morphology of the epidermis of the neotropical catfish Pimelodella lateristriga (Lichtenstein, 1823 with emphasis in club cells.

    Directory of Open Access Journals (Sweden)

    Eduardo Medeiros Damasceno

    Full Text Available The epidermis of Ostariophysi fish is composed of 4 main cell types: epidermal cells (or filament containing cells, mucous cells, granular cells and club cells. The morphological analysis of the epidermis of the catfish Pimelodella lateristriga revealed the presence of only two types of cells: epidermal and club cells. The latter were evident in the middle layer of the epidermis, being the largest cells within the epithelium. Few organelles were located in the perinuclear region, while the rest of the cytoplasm was filled with a non-vesicular fibrillar substance. Club cells contained two irregular nuclei with evident nucleoli and high compacted peripheral chromatin. Histochemical analysis detected prevalence of protein within the cytoplasm other than carbohydrates, which were absent. These characteristics are similar to those described to most Ostariophysi studied so far. On the other hand, the epidermal cells differ from what is found in the literature. The present study described three distinct types, as follows: superficial, abundant and dense cells. Differences among them were restricted to their cytoplasm and nucleus morphology. Mucous cells were found in all Ostariophysi studied so far, although they were absent in P. lateristriga, along with granular cells, also typical of other catfish epidermis. The preset study corroborates the observations on club cells' morphology in Siluriformes specimens, and shows important differences in epidermis composition and cell structure of P. lateristriga regarding the literature data.