Sample records for carbuncle

  1. Situación carbunclo rural en la Argentina 2011 -Condition of rural carbuncle in argentina 2011

    Directory of Open Access Journals (Sweden)

    Noseda, Ramon P.


    Full Text Available RESUMENDesde hace 8 años se elabora este informe sobre la Situación Anual del Carbunclo Rural producido por la bacteria esporulada Bacillus anthracis, siendo una zoonosis, enferma a animales y hombres relacionados con la labor ganadera.SUMMARYThis report of the Annual Condition of Rural Carbuncle produced by the spore bacteria Bacillus anthracis has been created every year since 2004. This zoonosis infects animals and people related to cattle labor.

  2. 姜树民教授从痈论治幽门螺杆菌阳性消化性溃疡经验%Experience of Professor JIANG Shu-min Treating Peptic Ulcer with Positive Hp Infection as Carbuncle

    Institute of Scientific and Technical Information of China (English)



    Professor JIANG Shu-min brings up the idea that treat peptic ulcer with positive Helicobacter pylori infection as carbuncle, he thinks peptic ulcer with positive Helicobacter pylori infection as internal-carbuncle, then makes treatments based on syndrome differentiation by using herbs which can regulate qi and activate blood circulation, and clear heat and detoxicate.%姜树民教授提出从痈论治幽门螺杆菌阳性的消化性溃疡,认为幽门螺杆菌阳性消化性溃疡乃内痈,运用理气活血、清热解毒药辨证施治.

  3. 溶血性巴斯德菌致面痈1例%A Case of Face Carbuncle Caused by Pasteurella Haemolytica

    Institute of Scientific and Technical Information of China (English)



    患者男,34岁.5年前因关节肿胀、疼痛被诊断为风湿性关节炎,不规范使用激素治疗半年.就诊时面部皮肤大部分有深红色炎性硬块,表面有多个穿孔,流出脓液及坏死组织,形成连片瘢痕.患者脓液检测出溶血性巴斯德菌.诊断:溶血性巴斯德菌感染致面痈.%A 34-year-old man had taken hormone therapy for half a year, because he was diagnosed as rheumatoid arthritis for joint swelling five years ago. When he came to hospital, most part of his face were affected by deep red and hard plaque lesions with multiple perforation, pus flow and necrotic tissues on surface, where the scar formed. Abscess culture obtained from the patient revealed pasteurella haemolytica. Diagnosis of pasteurella haemolytica caused face carbuncle was made

  4. 磁共振T2WI压脂及T1WI压脂增强序列在肛痈及肛漏诊断中的价值%The Value of Mri T2WI Pressure Grease and Fat Enhanced T1WI Pressure Sequence in Diagnosis of Anus Carbuncle and Anal Leakage

    Institute of Scientific and Technical Information of China (English)



    目的:分析磁共振T2WI压脂及T1WI压脂增强序列在肛痈及肛漏诊断中的价值。方法:41例肛痈及肛漏患者均行常规及Gd-DTPA增强磁共振检查,经手术证实,对以上各磁共振扫描序列组合对各种类型肛痈、肛漏及其内口的检出率进行统计分析。结果:肛痈32个,肛漏26个。对于肛痈、肛漏检出率,矢状位平扫及增强T1Wl压脂序列与手术结果比较差异均有统计学意义(P0.05)。结论:轴位联合冠状位T2WI压脂序列及T1WI压脂增强扫描序列可明显提高肛痈、肛漏临床诊断正确率,为手术方式的最佳选择提供科学信息。%Objective:To analyze the value of mri T2WI pressure grease and fat enhanced T1WI pressure sequence in diagnosis of anus carbuncle and anal leakage.Method:41 patients with anal carbuncle and anal leakage both conventional and Gd-DTPA enhanced magnetic resonance examination,confirmed by surgery,the combination of all mri scanning sequences of various types of anal abscess in mouth,anus leakage and its detection rate were analyzed. Result:Anal carbuncle was 32,anal leakage was 26.For abscess,anal leakage inspection rate,sagittal scan and enhanced T1Wl pressure grease series all had significant difference compared with surgical results(P0.05).Conclusion:Shaft joint coronary bits fat T2WI pressure fat sequence and T1WI enhancement scan sequence can obviously improve the anus carbuncle,anal leakage clinical diagnostic accuracy, provide scientific information for the best choice of the surgical procedure.

  5. Application of Muscle-Generating Powder in TCM Surgery for Sores and Carbuncle%生肌散在痈疡外科的应用

    Institute of Scientific and Technical Information of China (English)

    潘丽华; 杨启发


    @@ 历家医家对生肌散在痈疡外科中的应用均所有论述.笔者在多年的临床实践中,体会到该药药源广泛,取材方便,效果显著.并在原方的基础上,加味组成数种加味生肌散如下.

  6. The Touchstone to Test Human Nature--Read "The Great Carbuncle"%人性的"试金石"--《大红宝石》解读

    Institute of Scientific and Technical Information of China (English)

    方文开; 刘英男



  7. Drug: D07029 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ine formulations 52 Traditional Chinese medicines 520 Traditional Chinese 5200 Traditional Chinese medicines D07029 Hainosankyuto Traditional Chinese Medi...n; Carbuncle; Furuncle Therapeutic category: 5200 Therapeutic category of drugs in Japan [BR:br08301] 5 Crude drugs and Chinese medic

  8. Simulation of Compressible Multi-Phase Turbulent Reacting Flows (United States)


    positivity and entropy preservation. The known instability of this solver to odd-even decoupling and carbuncle phenomenon is cured by employing the HLLE...Two-Shock Riemann Solver and the Roe Riemann solver with Harten-Hyman entropy corrections showed very strong sensitiveness to the instability. The...Paper 2005–0314, 2005. [23] B. Fryxell, K. Olson, P. Ricker, F. X. Timmes, M. Zingale, D. Q. Lamb , P. Mac- Neice, R. Rosner, J. W. Truran, and H. Tufo

  9. 1,001 words and phrases you never knew you didn't know hopperdozer, hoecake, ear trumpet, dort, and other nearly forgotten terms and expressions

    CERN Document Server

    Runyan, W R


    As times change so do the things we use and what we call them. W.R. Runyan has compiled a fascinating collection of words and phrases that, once common everywhere in America, are little known today. This book is a celebration of our linguistic past that will be a treat from word lovers everywhere. Do you know any of these: Snaffle, Steelie, Gully Washer, Cabriolet, Calk, Hardpan, Carbuncle, Pie Bird, Fid, or Hame? Did you know that a "Horse Apple" is actually an orange? Or that "Rolley Holley" is a marble game? With over one hundred illustrations, this is a book that will take you on

  10. Effect of permeation enhancers on the release and permeation kinetics of Lincomycin hydrochloride gel formulations through mouse skin

    Directory of Open Access Journals (Sweden)

    Panigrahi L


    Full Text Available Lincomycin hydrochloride is a systemic antibiotic, which is active against most common gram positive bacteria. It has proved to be excellent for infectious diseases like acne, anthrax, pneumonia, and also for the treatment of furunculosis, carbuncles, impetigo, burns and wounds, carrying to gram positive bacteria. Gels were prepared using carbopol 940 as gelling agent, and isopropyl myristate and dimethyl sulfoxide as permeation enhancer. The formulations were evaluated for drug content, viscosity, pH, extrudability, homogeneity, skin irritation test, spreadability, and gel strength. A formulation containing 1.5% carbopol with 10% isopropyl myristate, showed better in vitro skin permeation through abdominal mouse skin, and was found to be the best.

  11. The pharmacological effects of Salvia species on the central nervous system. (United States)

    Imanshahidi, Mohsen; Hosseinzadeh, Hossein


    Salvia is an important genus consisting of about 900 species in the family Lamiaceae. Some species of Salvia have been cultivated world wide for use in folk medicine and for culinary purposes. The dried root of Salvia miltiorrhiza, for example, has been used extensively for the treatment of coronary and cerebrovascular disease, sleep disorders, hepatitis, hepatocirrhosis, chronic renal failure, dysmenorrhea, amenorrhea, carbuncles and ulcers. S. officinalis, S. leriifolia, S. haematodes, S. triloba and S. divinorum are other species with important pharmacological effects. In this review, the pharmacological effects of Salvia species on the central nervous system will be reviewed. These include sedative and hypnotic, hallucinogenic, skeletal muscle relaxant, analgesic, memory enhancing, anticonvulsant, neuroprotective and antiparkinsonian activity, as well as the inhibition of ethanol and morphine withdrawal syndrome.

  12. Adolpho Lutz and the origins of medical entomology in Brazil. (United States)

    Benchimol, J L


    Adolpho Lutz (1855-1940) formed a bridge between the Bahian Tropicalist School and post-Mansonian medicine. Before taking over as head of the São Paulo Bacteriological Institute (1893), Lutz traveled through a variety of regions and delved into various disciplines. In the 1880s, he was already arguing that leprosy was transmitted by mosquitoes. Carbuncles, cholera, and typhoid fever were then the accepted models for investigating the etiology of infectious diseases. Following the discovery of how malaria was transmitted, attention turned to hematophagous diptera. Physicians, bacteriologists, zoologists, and veterinarians reshaped the network of actors involved in the 'hunt' for the agents and transmitters of diseases, as they began relying on analogies with malaria and yellow fever. Edwin Ray Lankester, director of the British Museum (Natural History), launched then a worldwide investigation into species that might be linked to human disease. The species described by Lutz and his proposed classification system were vital to Frederick Theobald's fundamental work in medical entomology, published in the early twentieth century. In 1908, Lutz brought with him to the Oswaldo Cruz Institute a remarkable quantity of research and experiments in all branches of the newly created "tropical medicine," devoted especially to entomology.

  13. Cognitive-Enhancing Effect of Dianthus superbus var. Longicalycinus on Scopolamine-Induced Memory Impairment in Mice. (United States)

    Weon, Jin Bae; Jung, Youn Sik; Ma, Choong Je


    Dianthus superbus (D. superbus) is a traditional crude drug used for the treatment of urethritis, carbuncles and carcinomas. The objective of this study was to confirm the cognitive enhancing effect of D. superbus in memory impairment induced mice and to elucidate the possible potential mechanism. Effect of D. superbus on scopolamine induced memory impairment on mice was evaluated using the Morris water maze and passive avoidance tests. We also investigated acetylcholinesterase (AChE) activity and brain-derived neurotropic factor (BDNF) expression in scopolamine-induced mice. HPLC-DAD analysis was performed to identify active compounds in D. superbus. The results revealed that D. superbus attenuated the learning and memory impairment induced by scopolamine. D. superbus also inhibited AChE levels in the hippocampi of the scopolamine-injected mice. Moreover, D. superbus increased BDNF expression in the hippocampus. Eight compounds were identified using HPLC-DAD analysis. The content of 4-hydroxyphenyl acetic acid was higher than contents of other compounds. These results indicated that D. superbus improved memory functioning accompanied by inhibition of AChE and upregulation of BDNF, suggesting that D. superbus may be a useful therapeutic agent for the prevention or treatment of Alzheimer's disease.

  14. Organisms causing pyoderma and their susceptibility patterns

    Directory of Open Access Journals (Sweden)

    Baslas R


    Full Text Available Five hundred and seventy cases of pyoderma were studied clinically and bacteriologically. Of these, 58.8% cases were of primary pyoderma, and the rest were secondary pyoderma. Primary pyoderma consituted impetigo contagiosa (21.4%, bullous impetigo (3.3%, ecthyma (4.4%, superficial folliculitis (12.3%, chronic folliculitis of legs (8.8%; forunculosis (3.7% carbuncle (1.8%, folliculitis decalvans (0.4%, sycosis barbae (0.4% and abscess (2.5%. Secondary pyderma cases were infected scabies (23.9%, infected wound (1.1%, infectious eczematoid dermatitis (12.6%, intertrigo (0.4% and miscellaneous (3.3%. In 85 samples, no organism was isolated. Out of 485 samples, 75.9% grew a single organism and the rest (24.1% gave multiple organisms. Among the 603 strains collected, 73.6% were staphylococcus aureus, 25.0% were beta-haemolytic streptococcus and 0.7% each were alpha-haemolytic streptococcus and Gram negative bacilli. Eighty eight per cent strains of Staphylococcus aureous were susceptible to cephaloridine and 27.4% to ampicillin while 97.4% beta haemolytic streptococcus were susceptible to cephaloridine and 23.2% to pencillin.

  15. Bacteriological study of pyoderma with special reference to antibiotic susceptibility to newer antibiotics

    Directory of Open Access Journals (Sweden)

    Ghadage D


    Full Text Available Five hundred and forty-two cases of pyoderma were investigated to study bacterial aetiology and their antibiotic susceptibility patterns. Of these 65.87% cases were of primary pyoderma and the rest were of secondary pyoderma. Maximum cases were of impetigo (38.78% followed by folliculitis (12.92%, furunculosis (2.95%, ecthyma (3.5%, carbuncle (1.5% and sycosis barbae (0.4%. Secondary pyoderma constituted infected trophic ulcer (18.82%, infected pemphigus (7.2%, infected contact dermatitis (6.27%, and infected scabies (1.8%. Single organism was isolated from 46.9% cases and more than one type of organisms in 65.46% of cases. No organism was isolated in 5% of cases. Staphylococcus (67.34% was the predominant species isolated followed by beta-haemolytic streptococcus (21.77%. Maximum strains of Staph. aureus were susceptible to amikacin (75%, co-trimoxazole (72%, cefotaxime (65%, chloramphenicol (62%, ciprofloxacin (61% and clindamycin (61%. There was low susceptibility to cephaloridin (11%, gentamicin (12% and penicillin (21%. Streptococcus betahaemolyticus was highly sensitive to most of the antibiotics and less sensitive to cefotaxime (7%, co-trimoxazole (11% and penicillin (27%. Most of the strains were found to be resistant to one or more antibiotics.

  16. A robust HLLC-type Riemann solver for strong shock (United States)

    Shen, Zhijun; Yan, Wei; Yuan, Guangwei


    It is well known that for the Eulerian equations the numerical schemes that can accurately capture contact discontinuity usually suffer from some disastrous carbuncle phenomenon, while some more dissipative schemes, such as the HLL scheme, are free from this kind of shock instability. Hybrid schemes to combine a dissipative flux with a less dissipative flux can cure the shock instability, but also may lead to other problems, such as certain arbitrariness of choosing switching parameters or contact interface becoming smeared. In order to overcome these drawbacks, this paper proposes a simple and robust HLLC-type Riemann solver for inviscid, compressible gas flows, which is capable of preserving sharp contact surface and is free from instability. The main work is to construct a HLL-type Riemann solver and a HLLC-type Riemann solver by modifying the shear viscosity of the original HLL and HLLC methods. Both of the two new schemes are positively conservative under some typical wavespeed estimations. Moreover, a linear matrix stability analysis for the proposed schemes is accomplished, which illustrates the HLLC-type solver with shear viscosity is stable whereas the HLL-type solver with vorticity wave is unstable. Our arguments and numerical experiments demonstrate that the inadequate dissipation associated to the shear wave may be a unique reason to cause the instability.

  17. Surgical infection in art. (United States)

    Meakins, J L


    The earliest images of medicine and surgery in Western art are from the late Middle Ages. Although often attractive, at that time they were illustrative and mirrored the text on how to diagnose or treat a specific condition. These drawings in medieval manuscripts represent management of abscesses, perianal infection and fistulas, amputation, and wound dressings. With the Renaissance, art in all its forms flourished, and surgeons were represented at work draining carbuncles, infected bursae, and mastoiditis; managing ulcers, scrofula, and skin infections; and performing amputations. Specific diagnosis can be made, such as streptococcal infection in the discarded leg of the miraculous transplantation performed by Saints Cosmas and Damian and in the works of Rembrandt van Rijn and Frederic Bazille. Evocations of cytokine activity are evident in works by Albrecht Dürer, Edvard Munch, and James Tissot. The iconography of society's view of a surgeon is apparent and often not complimentary. The surgeon's art is a visual art. Astute observation leads to early diagnosis and better results in surgical infection and the septic state. Learning to see what we look at enhances our appreciation of the world around us but, quite specifically, makes us better clinicians.

  18. 浅析建筑材料在绿色建筑文化中的地位与作用%Position and Function of Building Materials in Green Architectural Culture

    Institute of Scientific and Technical Information of China (English)



    病态建筑(Sick building)是高速发展的毒痈,病态建筑综合症(Sick building syndrome)是人们享受城市生活的深痛.建筑的本源是给广大城市居民提供一个舒适、健康、充满活力、可持续发展的城市文化环境,是为提高人民小康生活的质量,是城市文化建设的根本出发点,病态建筑综合症给建筑必须绿色建绿色材料的选用问题上,拉响了警报.%Sick building is the high-speed development of the poison carbuncle. Sick building syndrome is the deep pain that people enjoy the city life. The building is to provide a comfortable, healthy, full of energy and sustainable development of the city culture environment, is to improve the quality of people's well-off life, is essential point of city culture construction. Sick building syndrome sounds the alarm in the selection of green architecture green material.

  19. Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice

    Institute of Scientific and Technical Information of China (English)

    XIAO Chenwen; HU Songhua; Rajput Zahid Iqbal


    The seed of the Momordica cochinchinensis (Lour.)Spreng as a traditional Chinese medicine has been utilized in China for more than 1 200 years.It is traditionally used for the treatment of inflammatory swelling,scrofula,tinea,diarrhea as well as suppurative skin infections such as sore,carbuncles,furuncles and boils in both humans and animals.In this study,an extract from Cochinchina momordica seeds(ECMS)is evaluated for its adjuvant effect and safety.The results suggest that when co-administered with ECMS in Balb/c mice,ovalbumin(OVA,10μg)may induce significantly higher specific antibody production than OVA used alone(P<0.05).Analysis of antibody isotypes indicates that the ECMS can promote the production of both IgG1 and IgG2a,concanavalin A,lipopolysaccharides or OVA are significantly higher in mice immunized with OVA mixed with ECMS than immunized with OVA alone or mixed with aluminum hydroxide(P<0.05).No local reactions and negative effects on the body weight gain occurred after the injection of OVA mixed with various amounts of ECMS in mice.Therefore,the ECMS is safe for injection and can be used as a potential vaccine adjuvant biasing the production of IgG2a in mice.

  20. Evidence for increasing severity of community-onset boils and abscesses in UK General Practice. (United States)

    Shallcross, L J; Hayward, A C; Johnson, A M; Petersen, I


    In England, hospital admissions for severe staphylococcal boils and abscesses trebled between 1989 and 2004. We investigated this trend using routine data from primary and secondary care. We used The Health Improvement Network (THIN), a large primary-care database and national data on hospital admissions from Hospital Episode Statistics (HES). Time trends in the incidence of primary-care consultations for boils and abscesses were estimated for 1995-2010. HES data were used to calculate age-standardized hospital admission rates for boils, abscesses and cellulitis. The incidence of boil or abscess was 450 [95% confidence interval (CI) 447-452] per 100 000 person-years and increased slightly over the study period (incidence rate ratio 1·005, 95% CI 1·004-1·007). The rate of repeat consultation for a boil or abscess increased from 66 (95% CI 59-73) per 100 000 person-years in 1995 to peak at 97 (95% CI 94-101) per 100 000 person-years in 2006, remaining stable thereafter. Hospital admissions for abscesses, carbuncles, furuncles and cellulitis almost doubled, from 123 admissions per 100 000 in 1998/1999 to 236 admissions per 100 000 in 2010/2011. Rising hospitalization and recurrence rates set against a background of stable community incidence suggests increased disease severity. Patients may be experiencing more severe and recurrent staphylococcal skin disease with limited treatment options.

  1. Related Study of SSTIs' ( Skin and Soft Tissue Infections ) External Therapy with Herbs%中药外敷治疗皮肤与软组织感染相关研究

    Institute of Scientific and Technical Information of China (English)

    张思嘉; 李萍; 王臬


    Objective: To summarize the related study of SSTIs' external therapy with traditional Chinese medicine in recent ten years.Methods: Using SSTIs, folliculitis, furuncle, carbuncle, lymphangitis, acute cellulites etc in western medicine while YONG, JUN, DING, JIE in tradition medicine as search words,1990-2010 as search years, CNKI, VIP, PubMed as search database, to search related literature about SSTIs, summarize and classify the therapy.Results: It is found that more than 50 literatures including clinical studies, experiment reports, representative cases.Conclusion: It is superiority to using external therapy with herbs to cure SSTIs.%目的:对近10年来中药外敷对皮肤软组织感染的治疗近况进行综述.方法:以西医:SSTIs、毛囊炎、疖、痈、淋巴管炎、急性蜂窝织炎、烧伤创面感染、手术后切口感染及褥疮感染;中医:痈、疽、疔、疖;中医外治等,为检索词,检索年限为1990-2010年,检索了中国知网数据库、重庆维普、清华同方、PubMed等,检索中医药对皮肤软组织感染的治疗近况文献,对其治疗方法进行分类并总结.结果:共搜索到50余篇中医文献.包括皮肤与软组织感染临床研究、实验报道、典型病例等.结论:中药外敷对皮肤与软组织感染的治疗有一定的优势.

  2. Adaptive finite-volume WENO schemes on dynamically redistributed grids for compressible Euler equations (United States)

    Pathak, Harshavardhana S.; Shukla, Ratnesh K.


    A high-order adaptive finite-volume method is presented for simulating inviscid compressible flows on time-dependent redistributed grids. The method achieves dynamic adaptation through a combination of time-dependent mesh node clustering in regions characterized by strong solution gradients and an optimal selection of the order of accuracy and the associated reconstruction stencil in a conservative finite-volume framework. This combined approach maximizes spatial resolution in discontinuous regions that require low-order approximations for oscillation-free shock capturing. Over smooth regions, high-order discretization through finite-volume WENO schemes minimizes numerical dissipation and provides excellent resolution of intricate flow features. The method including the moving mesh equations and the compressible flow solver is formulated entirely on a transformed time-independent computational domain discretized using a simple uniform Cartesian mesh. Approximations for the metric terms that enforce discrete geometric conservation law while preserving the fourth-order accuracy of the two-point Gaussian quadrature rule are developed. Spurious Cartesian grid induced shock instabilities such as carbuncles that feature in a local one-dimensional contact capturing treatment along the cell face normals are effectively eliminated through upwind flux calculation using a rotated Hartex-Lax-van Leer contact resolving (HLLC) approximate Riemann solver for the Euler equations in generalized coordinates. Numerical experiments with the fifth and ninth-order WENO reconstructions at the two-point Gaussian quadrature nodes, over a range of challenging test cases, indicate that the redistributed mesh effectively adapts to the dynamic flow gradients thereby improving the solution accuracy substantially even when the initial starting mesh is non-adaptive. The high adaptivity combined with the fifth and especially the ninth-order WENO reconstruction allows remarkably sharp capture of

  3. Assessment of nutritional status among adolescents: a hospital based cross sectional study

    Directory of Open Access Journals (Sweden)

    J. P. Singh


    Full Text Available Background: Adolescence, a period of transition between childhood and adulthood, occupies a crucial position in the life of human beings. The primary causes of under nutrition in India are its large population, socio-economic differences and inadequate access to health facilities. Nutritional assessments among adolescents are important as they are the future parents and constitute a potentially susceptible group. Studies on the assessment of nutritional status of adolescents are less in number and a National database has not yet been developed. Methods: The present hospital based cross sectional study was conducted in year 2013 among 344 rural adolescents of 10-19 years age (166 boys and 178 girls attending the outpatient department at rural health training centre (RHTC Dhaura Tanda, district Bareilly, Uttar Pradesh, belonging to the Muslim and Hindu caste communities. The nutritional status was assessed in terms of under nutrition (weight-for-age below 3rd percentile, stunting (Height-for-age below 3rd percentile and thinness (BMI-for-age below 5th percentile. Diseases were accepted as such as diagnosed by pediatrician, skin specialist and medical officer. Results: The prevalence of underweight, stunting and thinness were found to be 32.8%, 19.5% and 26.7% respectively. The maximum prevalence of malnutrition was observed among early adolescents (28%-47% and the most common morbidities were URTI (38.6%, diarrhea (16.8%, carbuncle / furuncle (16% and scabies (9.30%. Conclusion: The study concluded that the most common morbidities among adolescents were related to nutrition and personal hygiene. Regular health programmes should focus to educate and promote health among adolescent. [Int J Res Med Sci 2014; 2(2.000: 620-624

  4. Public awareness of testicular cancer and testicular self-examination in academic environments: a lost opportunity

    Directory of Open Access Journals (Sweden)

    Henry A. A. Ugboma


    Full Text Available BACKGROUND: Although testicular cancer is the most common cancer among 18- to 50-year-old males, healthcare providers seldom teach testicular self-examination techniques to clients, thus potentially missing opportunities for early detection. This form of cancer is easily diagnosable by testicular self-examination and is 96% curable if detected early. Periodic self-examination must be performed for early detection. Knowledge deficits and sociocultural norms contribute to low levels of health-related knowledge in most patients, resulting in undue delays before seeking medical advice. OBJECTIVE: Our aim is to assess the level of awareness of testicular cancer and the prevalence of the practice of testicular self-examination in academic environments to enable appropriate interventions. METHOD: A cross-sectional survey was administered to 750 consecutive males aged 18-50 years in three tertiary institutions in Port Harcourt from October 2008 to April 2009. RESULT: Knowledge or awareness of testicular cancer was poor. Almost all of the respondents were unaware that testicular lumps may be signs of cancer. A lump was typically construed as a benign carbuncle or something that could resolve spontaneously. The main factor contributing to respondents' lack of knowledge of testicular cancer was that few reported that they were "ever taught about testicular self-examination." CONCLUSION: Young adult men are unaware of their risk for testicular cancer, which is the most common neoplasm in this age group. Healthcare providers are not informing them of this risk, nor are they teaching them the simple early detection technique of self-examination of the testes.

  5. Suppressive effects of an ethanol extract of Gleditsia sinensis thorns on human SNU-5 gastric cancer cells. (United States)

    Lee, Se-Jung; Ryu, Dong Hee; Jang, Lee Chan; Cho, Seok-Cheol; Kim, Wun-Jae; Moon, Sung-Kwon


    The thorns of Gleditsia sinensis are a traditional Oriental medicine used for the treatment of swelling, suppuration, carbuncle and skin diseases. In the present study, we identified a novel molecular mechanism by which an ethanol extract of Gleditsia sinensis thorns (EEGS) inhibits the growth of the SNU-5 human gastric cancer cell line. EEGS treatment inhibited cell growth and was associated with G1 phase cell cycle arrest at a concentration of 400 µg/ml (IC50) in SNU-5 cells. Treatment with EEGS also stimulated p21WAF1 expression, which significantly decreased the expression of cyclins and cyclin-dependent kinases (CDKs). Further study suggested that p38 MAP kinase pathways may be involved in the inhibition of cell proliferation through p21WAF1‑dependent G1 phase cell cycle arrest in EEGS-treated cells. In addition, NF-κB and AP-1 transcription factor binding sites were identified as the cis-elements for tumor necrosis factor-α (TNF-α)-induced matrix metalloproteinase-9 (MMP-9) expression in SNU-5 cells, as determined by gel-shift assay. Treatment of cells with EEGS suppressed MMP-9 expression induced by TNF-α via a decrease in the binding activity of both NF-κB and AP-1 motifs. These data demonstrate that EEGS-mediated inhibition of cell growth appears to involve the activation of p38 MAP kinase, subsequently leading to the induction of p21WAF1 and the downregulation of cyclin D1/CDK4 and cyclin E/CDK2 complexes. Moreover, EEGS strongly inhibited TNF-α-induced MMP-9 expression by impeding the DNA binding activity of NF-κB and AP-1. Overall, these results provide a potential mechanism for EEGS in the treatment of gastric cancer.

  6. Development of an inviscid flux scheme for thermochemical nonequilibrium flow (United States)

    Campbell, Charles Hugh

    -Warming method is that stagnation region carbuncles are generated as a result of the flux errors identified in this work. Both of these hypothesis remain open for consideration, and are not validated in this thesis. Axisymmetric numerical simulations for a sphere at hypersonic reentry conditions are assessed for implementations of the Modified Steger-Warming and Flux Consistent inviscid flux schemes.

  7. New exploration on the types and forms of stone needle%砭石类型与形制新探

    Institute of Scientific and Technical Information of China (English)



    The basic use of stone needles in curing disease is cutting the carbuncles and purulences and pricking and draining off the sores. As a result, the forms of stone needle need to have a sharp cutting edge or slender point. Combining with corresponding unearthed material objects of archeology, the microlith-ie stone needle of mainly consists of a scraper, tip-like device and stone arrowheads. The stone needle of polished stone implements can be divided into three categories according to different forms and functions, that is conical-tip stone needles for pricking, e.g. lithostyle, coup de poing and stone arrowhead etc. ; sick-le-shaped needle stone for cutting, e.g. stone knife, stone sickle, stone adze and stone chisel etc; spear-shaped stone needle for both cutting and pricking, e.g. stone spear, stone sword, jade(stone) spear etc. From the usage cases recorded in the ancient literature, the conical-tip stone needle for pricking ought to be the most used of the ancient stone needles. Use of the stone needle for hot stroking, massage and knoc-king at the body surface, which was raised by some related scholars, does not belong to the domain of ancient stone needles.%砭石在治疗疾病中的基本用途是切割痈脓和刺泻瘀血,因而在形制上需具有锋利的刃口或细长的尖锋.结合相应的考古出土实物,细石器中的砭石,主要为刮削器、尖状器和石镞.磨制石器中的砭石,按照形制和功能可以分为三大类,即用于锥刺的锥尖状砭石,如石针、石锥、石镞等;用于切割的刀镰状砭石,如石刀、石镰、石锛、石凿等;兼具切割和锥刺功能的戈矛状砭石,如石矛、石剑、玉(石)戈等.从古代文献记载的使用情况来看,其中用于锥刺的锥尖状砭石应是古代砭石的主流.有关学者提出的用于熨烫、按摩及扣击体表的砭石,并不属于古代砭石的范畴.

  8. 《山海经》的医学地理学价值%The medical geographical value of the classic book Shan Hai Jing

    Institute of Scientific and Technical Information of China (English)

    龚胜生; 罗碧波


    《山海经》是我国现存最早的区域地理著作,《山经》各列山系中记载的疾病、药物一定程度上反映了当时疾病和药物的地理分布情况.大体面言,传染病主要流行于黄河中下游地区;精神与神经性疾病在豫西山地及南粤山地较为集中;地甲病和皮肤病主要分布在秦岭山脉;黄土高原外围的贺兰山、阴山、秦岭山脉分布着痈、疽、瘘、疣、痤等肿瘤、疮疡;湿病则主要分布在岭南地区和渭河南岸地区.《山海经》记载的药物和疾病,数量上可与《神农本草经》和《皇帝内经》相媲美,具有重要的医学地理学价值.%Shan Hai Jing is the existing earliest book of regional geography in China. As the most important part of the book, Shan Jing, which described the environment of series of mountains, recorded then many kinds of diseases and medicines (mainly herbs) , their places in series of mountains to some extent reflected their geographic distribution. In general, infectious diseases mainly occurred in middle and lower reaches of the Yellow River. Mental diseases and neurological diseases were more concentrated in the western mountains of Henan province and in the mountains of Guangdong province? Endemic goitre and skin diseases were mainly distributed in the Qinling Mountains, tumors such as carbuncle, gangrene, fistula, warts, acne, and sores were mainly distributed in Helanshan, Yinshan, Qinling Mountains around Loess Plateau. In brief, Shan Hai Jinghas important value of medical geography due to the number of recorded medicines and diseases which could be comparable with the both books Shen Nong's Herbal Classic and The Yellow Emperor's Classic of Internal Medicine.

  9. Flower-shaped ZnO nanoparticles synthesized by a novel approach at near-room temperatures with antibacterial and antifungal properties

    Directory of Open Access Journals (Sweden)

    Khan MF


    . Highly significant antimicrobial activity against medically important Gram-positive (Staphylococcus aureus and Gram-negative (Escherichia coli bacteria and fungi (Candida albicans by these ZNPs was also revealed. As S. aureus and C. albicans are responsible for many contagious dermal infections such as abscesses, furuncles, carbuncles, cellulitis, and candidiasis, we can postulate that our fabricated ZNPs may be useful as antimicrobial agents in antiseptic creams and lotions for the treatment of skin diseases. Keywords: antimicrobial activity, cetyl trimethyl ammonium bromide, flower-shape zinc oxide nanoparticles, near-room temperature, sol–gel method, skin disease

  10. 中医辨证论治肠痈38例疗效观察

    Institute of Scientific and Technical Information of China (English)



    The doctor of traditional Chinese medicine thinks intestinal carbuncle for“hot poison cohesion,blood stasis”in the colon.Clinical manifestation to “fever,abdominal pain,bag piece”as the primary symptom.Toxic heat accumulate bowel license performance “right lower abdomen tenderness,rebound ten-derness,red tongue moss yellow greasy,pulse number”,often in making“abscess detumescence,qingrejiedu,soft firm purge”.And qi stagnation and blood stasis is more performance “fixed do not move the right lower abdomen pain,often abdominal distention nausea,nausea,tongue ecchymosis,pulse string shibuya”,often in making “clear heat lishi,qi”;Toxic heat flourishing more performance “acute abdominal pain,fever,constipation,red tongue is purple and dry,pulse string”. Often in making \\“liver qi,qingrejiedu”.%中医认为肠痈多因“热毒内聚,瘀结肠中”而生。临床表现以“发热、腹痛、包块”为主症。热毒蕴肠证者多表现“右少腹触痛、反跳痛、舌红苔黄腻、脉滑数”,施治常以“排脓消肿、清热解毒、软坚通便”;气滞血瘀者多表现“右少腹疼痛固定不移、脘腹胀闷、恶心、舌有瘀斑、脉弦涩”,施治常以“清热利湿、行气活血”;热毒炽盛者多表现“腹痛剧烈、高热、大便秘结、舌红绛而干、脉弦数”。施治常以“疏肝理气、清热解毒”。

  11. Apoptotic effects of extract from Cnidium monnieri (L.) Cusson by adenosine monosphosphate-activated protein kinase-independent pathway in HCT116 colon cancer cells. (United States)

    Lim, Eun Gyeong; Kim, Guen Tae; Lee, Se Hee; Kim, Sang-Yong; Kim, Young Min


    Colon cancer, a common malignancy, can occur due to poor eating habits and increasing age. Consequently, careful regulation of eating habits may serve as a possible method for preventing the occurrence or progression of colon cancer. Extracts of the fruit of Cnidium monnieri (L.) Cusson are well‑known as an effective herbal medicine for the treatment of pain in female genitalia and carbuncle. However, there have been no studies on the apoptotic effects of Cnidium monnieri (L.) Cusson (CME). Adenosine monophosphate‑activated protein kinase (AMPK), the major regulator of energy metabolism, is activated by metabolic stress, including hypoxia and glucose deprivation. Activation of AMPK inhibits cell proliferation and induces apoptosis through the inhibition of phosphorylated (p)‑Akt and control of B‑cell lymphoma 2 (Bcl‑2) family members. The pro‑apoptotic proteins Bcl‑2‑associated X protein (Bax) and Bcl‑2‑homologous antagonist killer (Bak), are activated by their translocation to mitochondria from the cytosol. Translocation of Bax/Bak induces outer membrane permeabilization and is likely to lead to apoptosis through cytochrome C release and caspase activity. In the present study, the apoptotic effects and influence on mitochondria‑mediated apoptotic proteins of CME in HCT116 cells were assessed. We hypothesized that CME may have an effect on the inhibition of p‑Akt in an AMPK‑independent pathway. The present study demonstrated that CME induced the release of LDH and apoptosis through its inhibition of p‑Akt to control Bcl‑2 and activate Bax and Bak. Co‑treatment with CME and AMPK inhibitors showed that CME‑induced apoptosis does not occurr through a AMPK‑dependent pathway. Therefore, the present study determined, for the first time, that CME induced apoptosis as a result of causing metabolic stresses due to directly regulation of the de‑phosphorylation of Akt, whereas it did not control the AMPK-dependent pathway in HCT116

  12. 朱丹溪脾胃观源流与特征初探%A Preliminary Study on the Origins and Characteristics of Zhu Danxi's Concept of Spleen-Stomach

    Institute of Scientific and Technical Information of China (English)



    Objective] The article analyzed the origins of Zhu Danxi's concept of the spleen-stomach and the characteristics of the prescription, and explored the content of the academic research of Zhu Danxi. [Methods] Through literature method, the control analysis of the formation of succession context of Zhu Danxi's concept of the spleen-stomach, from three aspects, it analyzes the main characteristics of the view of the spleen and stomach on the physiology and pathology of spleen-stomach, spleen and stomach disease treatment, and treatment through the spleen-stomach.[Results] Zhu Danxi's concept of the spleen-stomach had the close relation with apprentice teacher and famous experts. The basic viewpoints origin from Liu Wansu, draws expelling spleen and stomach drug laws of Li Dongyuan, critically to retain the Zhang Congzheng's spleen-stomach academics, fully inherit the clinical experience of Luo Zhiti, and then form the concept of the spleen-stomach on physiological and the characteristics including the emphasis on physiological and pathological of the spleen-stomach and the prescription by protecting spleen-stomach. The treatment methods through the spleen-stomach to treat the exogenous and miscellaneous disease, including the tonifying spleen and regulating qi to dispel six stagnation, tonifying spleen and strengthening qi to remove exogenous, tonifying spleen and nourishing blood to resolute carbuncle and abscess, tonifying spleen and sweat to eliminate malaria cough, applicable law of clearing stomach and intestinal. [Conclusion] The formation and characteristics of Zhu Danxi's concept of the spleen-stomach is of great practical significance for guiding the clinical application and the cultivation of traditional Chinese medicine.%[目的]探讨朱丹溪脾胃观形成源流及其理法方药规律与特点,拓展丹溪学术研究内容。[方法]以文献学的方法,对照分析朱丹溪脾胃观形成的师承脉络,从脾胃的生理

  13. 糖尿病与火毒%Diabetes and Fire Toxin

    Institute of Scientific and Technical Information of China (English)

    黄世敬; 王永炎


    The objective of the paper is to explore the etiology,prevention and treatment of diabetes and fire toxin. Through literature analysis and clinical practice,it had been summarized that the formation and development of fire toxin presented in the entire progression of diabetes. Fire is classified as the physiological fire and pathological fire and the toxin is as the exogenous toxin and the endogenous toxin. Fire toxin in diabe-tes is related with improper diet(overeating greasy,sweet and strong flavor food,over - indulgence of food in fire toxin property,irregular food intake and unclean food),over - emotional changes(such as depression,an-ger,liver stagnation transforming into fire,grief,worry,hyperactivity of heart fire)and improper living condi-tion. Fire toxin is yang heat in nature and consumes qi. Flaring - up of fire toxin produces wind and stirs blood,disturbs heart mind and results in carbuncles. The upper jiao injury by fire toxin causes the upper xi-ao,the retention of fire toxin in the middle jiao causes the middle xiao and in the lower jiao causes diabetes of the lower xiao. Additionally,the transformations of fire toxin lead to the complications of diabetes. The proper diet is the origin of the disease prevention and the regular living habit protects the genuine qi. Clearing heat, eliminating toxin combined with nourishing yin,and reducing fire and removing toxin to consolidate the root are the treatment principles. All of these provide the reference for the study on the prevention and treatment of diabetes.%文章旨在探讨糖尿病火毒的成因与防治。通过文献分析,结合临床实践,总结出火毒的形成与演变贯穿于糖尿病始终。火有生理之火和病理之火之分,毒有外来之毒与内生之毒之别。糖尿病火毒形成与饮食不节(如过食肥甘厚味、偏食火毒性食物、饥饱无度、饮食不洁),情志过极(如郁闷恼怒、肝郁化火,忧愁思虑、心火亢盛)以及生

  14. 试析仲景《金匮要略》下法的应用特点%Application characteristics of the purgative method in Synopsis of Golden Chamber of Zhang Zhong-jing

    Institute of Scientific and Technical Information of China (English)

    田丽楠; 王新佩


    Purgative method, as one of the eight therapeutic methods of traditional Chinese medi-cine ( TCM) with many characteristics and advantages such as wide application and relatively quick effect. There are a lot of records and discussions on it in the ancient books of TCM. For example, there are more than 50 articles about purgative method and a total of 25 relevant prescriptions are involved in Synopsis of Golden Chamber. The relevant prescriptions includes Dachengqi Decoction, Xiaochengqi Decoction, Dahuang Xiaoshi Decoction, Zhizi Dahuang Decoction, Yinchenhao Decoction, Xiexin Decoction, Linggan Wuwei Decoction modified with Jiangxin Banxing Dahuang, Dahuang Gancao Decoction, Maziren Pill, Zhugaofa Decoction, Zouma Decoction, Dahuang Fuzi Decoction, Dahuang Zhechong Pill, Xiayuxue De-coction, Dahuang Mudan Decoction, Didang Decoction, Jiegengbai Powder, Shizao Decoction, Jijiao Li-huang Pill, Dahuang Gansui Decoction, Houpo Sanwu Decoction, Houpo Qiwu Decoction, Houpo Dahuang Decoction and Dachaihu Decoction. Besides, there are 17 kinds of diseases are treated with such method, including chest turgor, stomach turgor, bloody phlegm, jaundice, acute appendicitis, maternal abdominal pain, maternal intestinal carbuncle and maternal congestion of water and blood in blood chamber, so it is widely applied. This paper summed up the the provisions, diseases and syndromes, therapies, and pre-scriptions involving purgative method based on Synopsis of Golden Chamber, and discussed the application characteristics of the purgative method of ZHANG Zhong-jing from the following 6 aspects, such as nature of disease, diseased parts, exterior and interior of Yin and Yang, and medication features, in order to provide some reference for the diagnosis and treatment of the clinical diseases in modern TCM.%下法作为中医八大治疗方法之一,具有应用广泛、见效相对迅速的特点及优势。在中医古籍中有丰富的记载与论述。《金匮要略》中涉及下

  15. - Bacillus anthracis, utilización de un Sistema de Información Geográfico (SIG, para el análisis espacio temporal de 54 brotes de carbunclo rural en el partido de Azul, Bs. As., Argentina (Bacillus anthracis, use of a Geographical infoormation Service (GIS, for the temporary space analysis of 54 outbreaks of rural anthax in the county of Azul, Province of Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    Vazquez P.


    Full Text Available Resumen. El objeto de este trabajo es utilizar un Sistema de Información Geográfico (SIG para el análisis epidemiológico del Carbunclo Rural aplicado a un ecosistema ganadero, integrado por 618.000 bovinos, distribuidos en 1.350 establecimientos del partido de Azul, Provincia de Buenos Aires, Argentina. Esto permitió estudiar hechos epidemiológicos de una enfermedad de antigua data como es el Carbunclo Rural. Su agente causal el Bacillus anthracis, posee la característica de formar esporos que permite mantenerse durante decenas de años en el ambiente com capacidad de transmitir la enfermedad a otrois animales susceptibles. Se describe la metodología para su diagnóstico y los distintos elementos que integran el Sistema de Información Geográfico. Los 54 brotes identificados entre los años 1989 / 2005 fueron georreferenciados para posteriormente relacionarlos con las vías de avenamiento de aguas del partido de Azul, lãs características de sus suelos y la ubicación de las poblaciones de las poblaciones rurales que comparten la zona de isorriesgo. Se plantea un sistema de alerta epidemiológico temprano para establecimientos ganaderos que comparten vías de avenamiento de agua en común con el brote de Carbunclo diagnosticado. El 93 % de los brotes ocurrieron en establecimientos que comparten vías de avenamiento, mientras que el 7 % restante en establecimientos ganaderos ubicados fuera del área de influencia de estas vías Summary. The objjective of this study was the use of a Geographical Information Service (GIS for the epidemiologiacal analysis of rural Anthrax applied to a catle ecosystem, which includes 618.000 cattle, distributed on 1.350 farms in the county of Azul, Province of Buenos Aires, Argentina. This allowed studying long past epidemiological occurrences of a disease such as rural carbuncle Its causing egent is Bacillus anthracis, which has the property of forming spores That permit being maintained dozens of years in

  16. The Characteristics and the Modern Research of Potency Cold%药性寒的特点及现代研究

    Institute of Scientific and Technical Information of China (English)

    肖开; 苗明三


    Objective:To investigate the relationship between the potency cold and functionality,the pharmacological effects,Chinese medicine attending,modern medicine attending,Smell,Meridian,chemical composition.Methods:The application of "traditional Chinese medicine functions-pharmacology-clinical database system",the query" cold "with the frequency of association between the pharmacological effects,Smell,Meridian,the function,Chinese medicine attending,modern medicine attending,chemical composition,based on the number of the frequency,take the highest frequency covers 2/3 as the basic category,the highest frequency covers the remaining 2/ 3 as joint areas,remaining as expansion areas.Results:the basic functions of the potency cold are heat,detoxification,cooling blood,swelling;basic pharmacological effects of potency cold are anti-bacterial,anti-inflammatory,anti-tumor,antipyretic,diuretic,affect the cardiovascular system,lowering blood pressure,sedation,analgesic,anti-cancer; the TCM Indications of potency cold are carbuncles,sore throat,jaundice,erysipelas,external treatment of eczema,boils,eczema,Wind hot cold,the hot leaching,the hot leaching astringent pain,pharyngitis,nose bleeding,red eyes,sore,vomiting blood,Mumps,blood in the stool,edema fullness; the modern medicine attending of potency cold are burns,eczema,psoriasis,dysentery,hepatitis,coronary heart disease,hypertension,hyperlipidemia,constipation gallbladder inflammation,herpes zoster,cervical erosion,flat warts,gastric ulcer,chronic bronchitis,chronic bronchitis,urticaria,mumps,whooping cough;the basic Smell often combined with potency cold is sweet and bitter; the basic Meridian of potency cold are liver,lung,stomach,heart,kidney; the basic chemical composition of potency cold are organic acids,alkaloids,carbohydrates,inorganic substances,amino acids,glycosides,llavonoids,esters,tannins,volatile oils.Conclusion:To determine the relationship between the potency cold and functionality,the pharmacological

  17. 清热药的特点及现代研究%Modern Research and Relationship of Heat-clearing Traditional Chinese Medicine

    Institute of Scientific and Technical Information of China (English)

    李瑞奇; 白明; 苗明三


    Objective:To explore relationship between heat-c1earing traditional Chinese medicine and pharmacological action,traditional Chinese medical indications,modern medical indications,medicine properties,flavour of a medicine,channel tropism,chemical composition.Methods:Use the " properties and flavour of traditional Chinese medicine database systems ",inquiry associated frequency between function " Heat-clearing" and pharmacological action,traditional Chinese medical indications,modern medical indications,medicine properties,flavour of a medicine,channel tropism,chemical composition,according to the frequency,take cover 2/3 of most high frequency for basic area,take cover 2/3 of remaining most high frequency for joint area,remaining for extended area.Results:Basic pharmacological action of heat-clearing traditional Chinese medicine are antibacterial,anti-inflammatory,antitumor,antipyretic,calm,affecting the cardiovascular system,diuresis; TCM basic indications are carbuncle swollen,jaundice,sore throat pain and swelling,boils,vomiting blood,eyes red swelling and pain,heat astringent pain,nose bleeding,edema,inflammation of the throat,erysipelas,headache,snake bite,wind heat cold ; modern medicine basic indications are eczema,bacillary dysentery,hypertension,chronic bronchitis,colds,burns,whooping cough,stomach ulcers,hepatitis,mumps,pneumonia,herpes zoster,edema,pain and swelling of the throat,duodenal ulcer,suppurative otitis media,dermatitis,headache,coronary heart disease,bronchitis,verruca,acute tonsillitis,diarrhea,hyperlipidemia,cough,jaundice;basic medicine properties of heat-cleating TCM are cold,micro-cold;basic flavour of a medicine combined with heatclearing traditional Chinese medicine are bitter,sweet; basic channel tropisms are liver,lung,stomach,large intestine,heart;the basic chemical compositions of heat-cleating traditional Chinese medicine are organic acid,flavonoids,glycosides,alkaloids,sugars,tannins,amino acids,esters,volatile oil

  18. 朱槿花苞提取物治疗皮肤疖痈溃疡及其机制研究%Research in skin healing and repair function and mechanism of Hibiscus rosa-sinensis Linn bud extract

    Institute of Scientific and Technical Information of China (English)

    姜霁洋; 徐涵; 白雪婷; 程訸; 林艳玲; 凌震; 陈一村; 石刚刚


    目的:研究朱槿花苞提取物对小鼠皮肤疖痈溃疡模型的治疗作用,并对其可能作用机制进行探讨。方法将昆明小鼠分为模型组、阳性药组和朱槿花苞正丁醇萃取物( HrBN)组,采用小鼠疖痈模型,观察给药后实验结果。将昆明小鼠分为模型组、阳性药组和HrBN低、中、高剂量组,采用小鼠全层切除模型,观察给药后实验结果。通过体外抗菌实验检测朱槿花苞提取物抗菌MIC和抑菌圈。通过碳粒廓清实验测定朱槿花苞提取物对小鼠巨噬细胞廓清作用的影响,计算 K 和α值。结果 HrBN 对疖痈溃疡修复效果明显,并呈现量效关系。 HrBN、朱槿花苞乙酸乙酯萃取物( HrBE)及AB-8树脂体积分数0.3乙醇段、0.6乙醇段的抗菌作用明显。 HrBN及朱槿花苞乙醇提物( HrBA)高剂量能够增加K和α值。结论 HrBN在动物实验中体现了良好的皮肤疖痈溃疡修复作用,其疗效与抗菌活性和免疫增强作用可能有关。%Aim To test the skin healing and repairing efficacy and the mechanism of Hibiscus rosa-sinensis L bud extract by using the animal models. Methods KM mice were randomly divided into three groups:the model group, the positive control group, and the n-bu-tyl alcohol extract ( HrBN) group. Using the boils and carbuncles model, the healing condition of all the animals were observed. KM mice were kept in the SPF condition room and divided into five groups: the model group, the positive control group, and the low, middle, high dose groups. Using the full-thickness loss model, the repairing results of all the mice were ob-served. Through the antimicrobial test, the results of MIC and inhibition zone were obtained. The carbon clearance test was used to collect the blood at the time 5min and 15min, and get the liver and spleen, and the results of K andαwere obtained. Results In vivo ex-periments showed there was significant difference be-tween groups;the Hr

  19. 茉莉酸甲酯诱导大戟三萜类代谢的研究%Triterpene biosynthesis in Euphorbia pekinensis induced by methyl jasmonate

    Institute of Scientific and Technical Information of China (English)

    张文娟; 曹小迎; 蒋继宏


    京大戟是多年生草本药用植物,入药部分是其干燥根,但可入药的京大戟资源由于生长缓慢以及环境污染的加剧而越发匮乏,因此解决大戟资源日益紧张的问题是当今药用植物资源开发与利用方向的重要课题。京大戟含有三萜类、二萜类、黄酮类等丰富的活性成分,一些常见药用植物的有效成分是三萜类化合物,其在抗病毒、抗肿瘤、免疫调节等方面具有很好的活性。对植物萜类物质代谢起重要作用的关键酶,如3-羟基,3-甲基戊二酰辅酶 A 还原酶(hmgr)、鲨烯合酶(sqs)、法尼基焦磷酸合酶(fps)的基因克隆及活性研究取得了进展和突破,但通过调控萜类物质代谢途径中关键酶基因的表达来诱导终产物合成的研究鲜有报道。通过研究大戟萜类物质代谢途径进而利用基因工程手段提升目的物质的产量来解决京大戟药源短缺问题具有重要意义。该研究以大戟愈伤组织为材料,使用茉莉酸甲酯分别按时间梯度和浓度梯度进行诱导,将诱导后的愈伤组织分为两部分:一部分提取其总 RNA,以 actin 为内参基因进行反转录,实时定量 RT-PCR 分析大戟三萜类代谢途径中 hmgr、sqs 与 fps 基因的相对表达差异;另一部分用于提取其总三萜并使用分光光度法进行含量测定。实时定量 RT-PCR 分析结果表明,茉莉酸甲酯可诱导3个基因的表达,但其表达模式不一样。相应的京大戟愈伤组织中总三萜的含量明显提高,最高可较未处理样品增加27%。研究结果可为茉莉酸甲酯促进药用植物大戟三萜类物质积累的分子机制研究提供参考。%Euphorbia pekinensis is a perennial herb.The part of medicine is the dried root and is our traditional Chi-nese medicine.E .pekinensis have distributed in many provinces in China except for Tibet and Xinjiang.In recent years,medicine studies have showed that it can also be used on carbuncle