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Sample records for capulinia sp hemiptera

  1. A review of the genus Capulinia Signoret (Hemiptera: Coccoidea: Eriococcidae) with description of two new species.

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    Kondo, T; Gullan, P J; Cook, L G

    2016-05-17

    The eriococcid genus Capulinia Signoret currently comprises four Neotropical species (the type species C. sallei Signoret, C. crateraformis Hempel, C. jaboticabae Ihering and an undescribed species recognised in the literature) and one species from New Zealand (C. orbiculata Hoy). All species feed on plants in the family Myrtaceae and the undescribed species is a pest of guava, Psidium guajava, in Venezuela and Colombia. Here we describe the pest species based on the adult female and first-instar nymph and name it Capulinia linarosae Kondo & Gullan sp. n. We provide a summary of published information on the biology and pest status of C. linarosae by translating the Spanish literature. We also describe the adult female and first-instar nymph of a new Argentine species that we name as C. luma Kondo & Gullan sp. n. after its host Luma apiculata. In addition, we redescribe the adult female of C. jaboticabae and include notes on C. crateraformis, C. orbiculata and C. sallei. We provide a revised generic diagnosis and keys to all Capulinia species based on adult females and, where available, first-instar nymphs, as well as a revised key to South American eriococcid genera. Phylogenetic analyses of 18S rDNA place Capulinia within the "Gondwanan" clade of eriococcids, mostly likely within the Myrtaceae-feeding group.

  2. A new Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis (Cactaceae).

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    Triapitsyn, Serguei V; Aguirre, María B; Logarzo, Guillermo A

    2016-05-26

    A new species of Anagyrus Howard (Hymenoptera: Encyrtidae), A. lapachosus sp. n., is described from Salta Province of Argentina as a parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis cactus (Cactaceae). It is a candidate "new association" biological control agent for quarantine evaluation and possible following introduction to Puerto Rico (USA) against another Hypogeococcus sp., commonly called the Harrisia cactus mealybug and often misidentified as H. pungens Granara de Willink (according to our unpublished data the latter attacks only Amaranthaceae), which devastates or threatens the native cacti there and also in some other Caribbean islands (Triapitsyn, Aguirre et al. 2014; Carrera-Martínez et al. 2015).

  3. Description of Sangeeta sinuomacula sp. nov. (Hemiptera: Cicadellidae: Megophthalminae: Agalliini) from Yunnan Province of Southwest China.

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    Li, Hu; Dai, Ren-Huai; Li, Zi-Zhong

    2015-01-01

    A new species, Sangeeta sinuomacula Li, Dai & Li sp. nov., of tribe Agalliini of subfamily Megophthalminae (Hemiptera: Cicadellidae) is described and illustrated from Yunnnan Province of Southwest China. The new species is easily distinguished from other Sangeeta species by the aedeagal shaft with a pair of slender processes instead of lamelliform lateral expansions. A key to Sangeeta species and updated checklist with distribution are provided.

  4. Anagrus turpanicus sp. n. (Hymenoptera: Mymaridae) from China, an egg parasitoid of Arboridia kakogowana (Hemiptera: Cicadellidae).

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    Hu, Hong-Ying; Triapitsyn, Serguei V

    2016-01-01

    A new Palaearctic species of Anagrus Haliday (Hymenoptera: Mymaridae), A. turpanicus Triapitsyn & Hu sp. n., is described and illustrated from Xinjiang Uyghur Autonomous Region of China. It was reared from parasitized eggs of the leafhopper Arboridia kakogowana (Matsumura) (Hemiptera: Cicadellidae) (Fig. 10) on cultivated table grapes from Turpan, which had been previously misidentified there as Erythroneura apicalis (Nawa) (e.g., Wang et al. 2004, 2011; Luan et al. 2006). This leafhopper has been an important economic pest in Turpan area since 1998, causing serious damage to the cultivated grapevines (Wang et al. 2004; Luan et al. 2006). Wang et al. (2011) reported that the mite Leptus sp. (Erythraeidae) and several unidentified spider species were the main natural enemies of Erythroneura apicalis in and around Turpan. This is the first record of A. kakogowana from China; it was not included in the key to the Chinese species of the genus Arboridia Zachvatkin by Song & Li (2015). Arboridia kakogowana is native to the eastern Palaearctic region (Japan, Korea, and Far East of Russia), and has been recently recorded as an invasive pest of cultivated grapes in southern Russia (Gnezdilov et al. 2008).

  5. POPULATION FLUCTUATION OF Empoasca sp. (HEMIPTERA: CICADELLIDAE IN A PHYSIC NUT CROP IN MATO GROSSO DO SUL

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    Denisar Paggioli de Carvalho

    2015-11-01

    Full Text Available ABSTRACTPhysic nut (Jatropha curcas L. is an oilseed, semi-evergreen shrub or small tree of the Euphorbiaceae family, whose seeds contain oil that can be processed into a high quality biofuel. However, there have been reports of arthropods feeding from its leaves, including the green leafhopper Empoasca sp. (Hemiptera: Cicadellidae. The large numbers of this insect, observed in certain periods of the year in many regions of Brazil, are causing damage to the oilseed crops. This study aims at evaluating the fluctuation in green leafhopper population in a physic nut crop in Dourados, Mato Grosso do Sul, to assess possible correlations with rainfall, maximum, average and minimum temperatures. This evaluation was conducted between March 2011 and July 2012. The largest Empoasca sp. populations were recorded in May and June, 2011, and between February and May, 2012. No significant correlation was observed between the weather parameters analyzed and the fluctuation in the Hemiptera population, but there was a trend toward higher population density during the warmer and rainier months.RESUMENEl piñón manso (Jatropha curcas L. es una oleaginosa de la familia Euphorbiaceae que se destaca por la producción de semillas cuyo aceite tiene características deseables para la producción de biocombustibles. Sin embargo, hay informes de algunos artrópodos que usan la planta como fuente de alimento, incluyendo la cigarrita verde Empoasca sp. (Hemiptera: Cicadellidae. La alta incidencia de este insecto se comprueba en varias regiones del Brasil, en ciertas épocas del año, causando lesiones a esta oleaginosa. El objetivo de este estudio fue evaluar la fluctuación de la cigarrita verde en una plantación de piñón manso en Dourados, Mato Grosso do Sul, y la búsqueda de posibles correlaciones con las precipitaciones y las temperaturas máximas, medias y mínimas. Esta evaluación se realizó entre los meses de marzo 2011 hasta julio 2012. Poblaciones mayores

  6. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

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    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  7. Primeiro registro de ocorrência do parasitóide Brasema sp. (hymenoptera: eupelmidae em ovos de Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae no Brasil First record of occurrence of the parasitoid Brasema sp. (hymenoptera: eupelmidae in eggs of Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae in Brazil

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    Carlos Henrique Marchiori

    2002-12-01

    Full Text Available Este trabalho registra a primeira ocorrência do parasitóide Brasema sp. (Hymenoptera: Eupelmidae parasitando ovos de Leptoglossus zonatus (Hemiptera: Coreidae em cultivar de milho em Itumbiara, GO. A porcentagem de parasitismo foi de 4,8%. Brasema sp. constitue-se em ectoparasitóide de larvas de coleópteros e de outros hospedeiros no interior de tecidos de plantas.This work reports, for the first time, of parasitoid Brasema sp. (Hymenoptera: Eupelmidae parasitizinf eggs of Leptoglossus zonatus (Hemiptera: Coreidae in maize cultivate in Itumbiara, GO, Brazil. The percentage of parasitizing was 4,8%. Brasema sp. constitutes ectoparasitoid of beetle larvae and other hosts concealed in plant tissue.

  8. Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae

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    Teresa Cristina Monte Goncalves

    2013-06-01

    Full Text Available Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is distinguished by its general colouring, differences in the blotches on the connexivum, wing size in females and external structures of the male genitalia. The type series has been deposited in the Entomological Collection and Herman Lent Collection, Oswaldo Cruz Institute-Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

  9. Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae)

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    Gonçalves, Teresa Cristina Monte; Teves-Neves, Simone Caldas; dos Santos-Mallet, Jacenir Reis; Carbajal-de-la-Fuente, Ana Laura; Lopes, Catarina Macedo

    2013-01-01

    Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is distinguished by its general colouring, differences in the blotches on the connexivum, wing size in females and external structures of the male genitalia. The type series has been deposited in the Entomological Collection and Herman Lent Collection, Oswaldo Cruz Institute-Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. PMID:23828010

  10. Description of Rhodnius marabaensis sp. n. (Hemiptera, Reduviidae, Triatominae) from Pará State, Brazil

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    Souza, Eder dos Santos; Von Atzingen, Noé Carlos Barbosa; Furtado, Maria Betânia; de Oliveira, Jader; Nascimento, Juliana Damieli; Vendrami, Daniel Pagotto; Gardim, Sueli; da Rosa, João Aristeu

    2016-01-01

    Abstract Rhodnius marabaensis sp. n. was collected on 12 May 2014 in the Murumurú Environmental Reserve in the city of Marabá, Pará State, Brazil. This study was based on previous consultation of morphological descriptions of 19 Rhodnius species and compared to the identification key for the genus Rhodnius. The examination included specimens from 18 Rhodnius species held in the Brazilian National and International Triatomine Taxonomy Reference Laboratory in the Oswaldo Cruz Institute in Rio de Janeiro, Brazil. The morphological characteristics of the head, thorax, abdomen, genitalia, and eggs have been determined. Rhodnius prolixus and Rhodnius robustus were examined in more detail because the BLAST analysis of a cyt-b sequence shows they are closely related to the new species, which also occurs in the northern region of Brazil. The most notable morphological features that distinguish Rhodnius marabaensis sp. n. are the keel-shaped apex of the head, the length of the second segment of the antennae, the shapes of the prosternum, mesosternum and metasternum, the set of spots on the abdomen, the male genitalia, the posterior and ventral surfaces of the external female genitalia, and the morphological characteristics of the eggs. Rhodnius jacundaensis Serra, Serra & Von Atzingen (1980) nomen nudum specimens deposited at the Maraba Cultural Center Foundation - MCCF were examined and considered as a synonym of Rhodnius marabaensis sp. n. PMID:27833419

  11. A new species of Paraphaenodiscus Girault (Hymenoptera: Encyrtidae from India parasitizing Coccus sp. (Hemiptera: Coccidae

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    Sudhir Singh

    2016-02-01

    Full Text Available A new species, Paraphaenodiscus udayveeri Singh sp. nov., has been described and illustrated with automontaged photographs of both male and female. Species parasitized scale insects on the leaves of Pterygota alata which were weaved into nest of red weaver ant, Oecophylla smaragdina (Hymenoptera: Formicidae. Species is compared with P. chrysocomae Prinsloo and P. pedanus Prinsloo & Mynhardt. Key to world species of Paraphaenodiscus except European species is also given. Types are deposited with National Forest Insect Collection, Entomology Division, Forest Research Institute, Dehra Dun, India (NFIC-FRI.

  12. Anoeconeossa bundoorensis sp. n., a new psyllid (Hemiptera: Psylloidea) from Eucalyptus camaldulensis (Myrtaceae) from Southeast Australia.

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    Taylor, Gary S; Farnier, Kevin; Burckhardt, Daniel; Steinbauer, Martin J

    2013-01-30

    Anoeconeossa bundoorensis sp. n. is described from Eucalyptus camaldulensis (Myrtaceae) from southern Victoria in Southeast Australia. It is placed in the A. communis Taylor species-group as the paramere lacks combs of black rods. It differs from other members of the species-group, A. communis and A. bullata Taylor as it lacks an anterobasal expansion on the paramere and from A. unicornuta Taylor as the inner horn-shaped process of the paramere is reduced to a short spine and the apical expansion is more elongate, with a corresponding greater length of equidistant setae. Taxonomically relevant morphological details are illustrated and the species is diagnosed from other eucalypt inhabiting congeners. In-formation on the biology is presented.

  13. Halophilanema prolata n. gen., n. sp. (Nematoda: Allantonematidae, a parasite of the intertidal bug, Saldula laticollis (Reuter(Hemiptera: Saldidae on the Oregon coast

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    Poinar George O

    2012-02-01

    Full Text Available Abstract Background It is rare to find terrestrial nematode lineages parasitizing arthropods inhabiting the intertidal or littoral zone of the oceans. During an ecological study along the Oregon dunes, an allantonematid nematode (Tylenchomorpha: Allantonematidae was discovered parasitizing the intertidal shore bug, Saldula laticollis (Reuter(Hemiptera: Saldidae. This shore bug is adapted to an intertidal environment and can survive short periods of submergence during high tides. The present study describes the nematode parasite and discusses aspects of its development, ecology and evolution. Methods Adults and last instar nymphs of S. laticollis (Hemiptera: Saldidae were collected from the high intertidal zone among clumps of Juncus L. (Juncaceae plants at Waldport, Oregon on October 3, 2011. The bugs were dissected in 1% saline solution and the nematodes killed in 1% Ringers solution and immediately fixed in 5% formalin (at 20°C. Third stage juveniles removed from infected hosts were maintained in 1% saline solution until they matured to the adult stage, molted and mated. Results Halophilanema prolata n. gen., n. sp. (Nematoda: Allantonematidae is described from last instar nymphs and adults of the intertidal bug, Saldula laticollis on the Oregon coast. The new genus can be distinguished from other genera in the Allantonematidae by a stylet lacking basal knobs in both sexes, an excretory pore located behind the nerve ring, ribbed spicules, a gubernaculum, the absence of a bursa and the elongate-tubular shape of the ovoviviparous parasitic females. Studies of the organogenesis of Halophilanema showed development to third stage juveniles in the uterus of parasitic females. Maturation to the free-living adults and mating occurred in the environment. The incidence of infection of S. laticollis ranged from 0% to 85% depending on the microhabitat in the intertidal zone. Conclusions Based on the habitat and morphological characters, it is proposed

  14. Fauna Triatominae do Estado de Bahia, Brasil.V - Microtriatoma pratai sp. n. Hemiptera, Reduviidae, Triatominae, Bolboderini Triatomidae fauna of the State of Bahia, Brazil. V - Microtriatoma pratai sp. n. - Hemiptera,Reduviidae, Triatominae, Bolderini

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    Ítalo A. Sherlock

    1982-06-01

    Full Text Available Microtriatoma pratai sp. n. de Hemíptera, Reduviidae, Triatominae, Bolboderini, é descrita no presente trabalho com base em um exemplar coletado em domicílio da cidade do Salvador, Estado da Bahia, Brasil. A espécie se distingue facilmente das outras conhecidas do gênero, principalmente por apresentar asas sem manchas e conexivo com largas manchas claras e escuras alternadas.Microtriatoma pratai n. sp. (Hemíptera, reduviidae, Triatominae, Bolboderini is described from a single specimen collected inside a house in the city of Salvador, State of Bahia, Brazil. This species is easily distinguished from the others belonging to the genus principally by the existence of alternating broad light and dark coloured spots on the connexivum and the absence of spots on the hemelytra.

  15. Morphology, songs and genetics identify two new cicada species from Morocco: Tettigettalna afroamissa sp. nov. and Berberigetta dimelodica gen. nov. & sp. nov. (Hemiptera: Cicadettini).

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    Costa, Gonçalo João; Nunes, Vera L; Marabuto, Eduardo; Mendes, Raquel; Laurentino, Telma G; Quartau, José Alberto; Paulo, Octávio S; Simões, Paula Cristina

    2017-03-01

    Morocco has been the subject of very few expeditions on the last century with the objective of studying small cicadas. In the summer of 2014 an expedition was carried out to Morocco to update our knowledge with acoustic recordings and genetic data of these poorly known species. We describe here two new small-sized cicadas that could not be directly assigned to any species of North African cicadas: Tettigettalna afroamissa sp. nov. and Berberigetta dimelodica gen. nov. & sp. nov. In respect to T. afroamissa it is the first species of the genus to be found outside Europe and we frame this taxon within the evolutionary history of the genus. Acoustic analysis of this species allows us to confidently separate T. afroamissa from its congeners. With B. dimelodica, a small species showing a remarkable calling song characterized by an abrupt frequency modulation, a new genus had to be erected. Bayesian inference and maximum likelihood phylogenetic analyses with DNA-barcode sequences of Cytochrome C Oxidase 1 support the monophyly of both species, their distinctness and revealed genetic structure within B. dimelodica. Alongside the descriptions we also provide GPS coordinates of collection points, distributions and habitat preferences.

  16. [Triatoma vandae sp.n. of the oliveirai complex from the State of Mato Grosso, Brazil (Hemiptera: Reduviidae: Triatominae)].

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    Carcavallo, Rodolfo U; Jurberg, José; Rocha, Dayse da Silva; Galvao, Cleber; Noireau, François; Lent, Herman

    2002-07-01

    There are several specific complexes belonging to the genus Triatoma Laporte, 1832, which are generally associated to specific geographic areas. Recent publications have linked the oliveirai complex to ecosystems of Mato Grosso, which are also present in other Brazilian states and even in other bordering countries as eastern Paraguay. The study of the abundant material collected during the last years allowed the description of several new species of the oliveirai complex: T. jurbergi Carcavallo, Galvão Lent, 1998; T. baratai Carcavallo Jurberg, 2000 and T. klugi Carcavallo, Jurberg, Lent Galvão, 2001. Another new species belonging to the same complex is described here as T. vandae sp.n. It originates from the state of Mato Grosso, and has been reared in the insectary of the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Departamento de Entomologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro.

  17. Revision of the Neotropical treehopper genus Tolania (Hemiptera, Membracidae

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    Jesse L. Albertson

    2006-12-01

    Full Text Available The treehopper genus Tolania Stål (Hemiptera: Membracidae: Nicomiinae: Nicomiini and 69 valid species (59 new are described and illustrated based on adult morphology. Ten informal species groups are recognized based on a previously published phylogenetic analysis: (1 the dira species group comprising T. calista sp. nov., T. dira sp. nov., T. inca sp. nov., T. macaria sp. nov., T. secoya sp. nov., and T. zaparo sp. nov.; (2 the fasciata species group comprising T. fasciata (Walker, T. gracilis sp. nov., and T. laticlava sp. nov.; (3 the femoralis species group comprising T. femoralis Stål, T. fraterna Stål, and T. roberti sp. nov.; (4 the furcata species group comprising T. furcata sp. nov., T. tryphena sp. nov., and T. venezuelensis sp. nov.; (5 the hispida species group comprising T. alida sp. nov., T. hispida sp. nov., and T. periculosa sp. nov.; (6 the malefica species group comprising T. cactina sp. nov., T. curvata sp. nov., T. grallator sp. nov., T. jocosa sp. nov., T. mackameyi sp. nov., T. malefica sp. nov., T. obliqua (Walker, and T. terencia sp. nov.; (7 the obtusa species group comprising T. obtusa Fowler, T. obunca sp. nov., and T. torosa sp. nov.; (8 the opponens species group comprising T. alvira sp. nov., T. arcuata sp. nov., T. damia sp. nov., T. insolita sp. nov., T. lunata sp. nov., T. lurida sp. nov., T. opponens (Walker, T. oriana sp. nov., T. reflexa sp. nov., T. risa sp. nov., T. sinuata sp. nov., T. trilobata sp. nov., T. tumida sp. nov., T. umbella sp. nov., T. vitocensis sp. nov., T. woodi sp. nov., and T. xantha sp. nov.; (9 the peltacauda species group comprising T. brasiliensis sp. nov., T. iratafelis sp. nov., T. modesta sp. nov., T. peltacauda sp. nov., T. picta sp. nov., and T. thyrea sp. nov.; and (10 the semipellucida species group comprising T. atrata sp. nov., T. fimbriata sp. nov., T. nicia sp. nov., and T. semipellucida Stål. The following new species are not placed in species groups: T. anomala sp. nov

  18. Using a kairomone-based attracting system to enhance biological control of mealybugs (Hemiptera: Pseudococcidae by Anagyrus sp. near pseudococci (Hymenoptera: Encyrtidae in Sicilian vineyards

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    Ramzi Mansour

    2010-11-01

    Full Text Available The “potato trap” technique was applied for the fi rst time in Italian (Sicily vineyards in an attempt to assess: a the impact of the kairomonal activity of the vine mealybug sex pheromone (S-(+-lavandulyl senecioate (LS on the parasitism of mealybugs by the encyrtid Anagyrus sp. near pseudococci, b the influence of two commonly used insecticides on the parasitization activity of A. sp. near pseudococci towards mealybugs, and c the efficiency of the release of A. sp. near pseudococci in enhancing parasitism rates of mealybugs. The number of captured A. sp. near pseudococci females in LS baited traps was significantly higher than that in unbaited traps. The minimal number of days for the first parasitoid emergence in LS baited traps was almost 3 days earlier, compared to unbaited control, suggesting a faster host detection by the encyrtid when LS is applied. These findings resulted in a significant increase in parasitism of mealybugs by A. sp. near pseudococci in LS baited traps relative to unbaited traps suggesting that the LS is used by the encyrtid as kairomone to ensure greater potential for host searching activity. Insecticide treatments significantly affected parasitization activity of A. sp. near pseudococci on mealybugs when compared to an untreated control with parasitoid release. The buprofezin, chlorpyriphos-methyl and untreated control with no parasitoid release treatments had statistically similar numbers of emerged parasitoids from exposed mealybugs. The obtained results provide evidence that, in the absence of conventional insecticides applications, the use of the LS could be a promising tool to improve and strengthen biological control of mealybugs by A. sp. near pseudococci within Sicilian vineyard conditions.

  19. Characteristics, phenotype, and transmission of Wolbachia in the sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), and its parasitoid Eretmocerus sp. nr. emiratus (Hymenoptera: Aphelinidae).

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    Chiel, Elad; Kelly, Suzanne E; Harris, Alexandre M; Gebiola, Marco; Li, Xianchun; Zchori-Fein, Einat; Hunter, Martha S

    2014-04-01

    Wolbachia is a common intracellular bacterial endosymbiont of insects, causing a variety of effects including reproductive manipulations such as cytoplasmic incompatibility (CI). In this study, we characterized Wolbachia in the whitefly Bemisia tabaci and in the whitefly parasitoid Eretmocerus sp. nr. emiratus. We also tested for horizontal transmission of Wolbachia between and within trophic levels, and we determined the phenotype of Wolbachia in E. sp. nr. emiratus. Using multilocus sequence typing and phylogenetic analyses, we found that B. tabaci and E. sp. nr. emiratus each harbor a different and unique strain of Wolbachia. Both strains belong to the phylogenetic supergroup B. No evidence for horizontal transmission of Wolbachia between and within trophic levels was found in our study system. Finally, crossing results were consistent with a CI phenotype; when Wolbachia-infected E. sp. nr. emiratus males mate with uninfected females, wasp progeny survival dropped significantly, and the number of females was halved. This is the first description of CI caused by Wolbachia in the economically important genus Eretmocerus. Our study underscores the expectation that horizontal transmission events occur rarely in the dynamics of secondary symbionts such as Wolbachia, and highlights the importance of understanding the effects of symbionts on the biology of natural enemies.

  20. Description of a new coccid (Hemiptera, Coccidae on avocado (Persea americana Mill. from Colombia, South America

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    Takumasa Kondo

    2010-04-01

    Full Text Available A new soft scale insect, Bombacoccus aguacatae Kondo, gen. n. and sp. n. (Hemiptera: Coccidae collected on the branches and twigs of avocado, Persea americana Mill. (Lauraceae in Colombia, is described and illustrated based on the adult female. An updated taxonomic key to closely related genera of the Toumeyella-group is provided.

  1. Atividade inseticida de Piper aduncum L. (Piperaceae sobre Aetalion sp. (Hemiptera: Aetalionidae, praga de importância econômica no Amazonas Insecticidal activity of Piper aduncum L. (Piperaceae on Aetalion sp. (Hemiptera: Aetalionidae, plague of economic importance in Amazon

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    Wilson Castro Silva

    2007-06-01

    Full Text Available O uso de extratos de plantas, em grande parte ainda inexplorada na Amazônia, constitui uma alternativa para o controle de insetos fitófagos, devido o baixo custo operacional, facilidade de preparação, utilização e segurança para o meio ambiente. O objetivo do presente trabalho foi avaliar a ação inseticida de Piper aduncum sobre Aetalion sp. Insetos adultos, coletados em Clitoria fairchildiana, foram separados em grupos de dez indivíduos, colocados em recipientes plásticos e expostos à aplicação tópica de extratos aquosos de folhas e raízes de P. aduncum. Os extratos foram aplicados nas concentrações de 10, 20 e 30 mg.ml-1. Os grupos de controle tratados com água destilada. Os testes tiveram 48 horas de duração e a cada 12 horas a porcentagem de mortalidade foi avaliada. O experimento se caracterizou num delineamento inteiramente ao acaso com três tratamentos em cinco repetições mais o grupo controle. O extrato de folhas de P. aduncum apresentou menor toxicidade (CL50 = 20,9 mg.ml-1 do que o extrato de raízes (CL50 = 20,2 mg.ml-1, mas não foram estatisticamente distintos. Tanto o extrato aquoso de raízes como o de folhas de P. aduncum apresentam atividade inseticida sobre adultos de Aetalion sp. Entretanto, por não causar impacto ambiental durante a coleta, recomenda-se o extrato de folhas em programas de controle alternativo desse inseto.The use of extracts of plants, usually under exploited in Amazonia, is an alternative to control phytophagous insects due to their low costs, easy preparation, use and environmental safety. The aim of this work was to evaluate the insecticidal action of P. aduncum on Aetalion sp. Adult insects collected on the Clitoria fairchildiana were separated in groups of ten individuals into plastic vessels and exposed to topic application of aqueous extracts of leaves and roots of P. aduncum. The extracts were applied at the concentrations of 10, 20 and 30 mg.ml-1. The control groups treated

  2. Studies on idiocerine leafhoppers with descriptions of Chinaocerus gen. nov. and three new species from China (Hemiptera: Cicadomorpha: Cicadellidae).

    Science.gov (United States)

    Dang, Li-Hong; Zhang, Bin; Li, Zi-Zhong

    2016-01-01

    A new leafhopper genus of the tribe Idiocerini (Hemiptera: Cicadomorpha: Cicadellidae), Chinaocerus, is described from the southwest China together with three new species, C. kangdingensis Zhang & Li sp. nov. (Sichuan Province), C. bispinatus Zhang & Li sp. nov. (Yunnan Province) and C. shii Zhang & Li sp. nov. (Sichuan Province). Descriptions and illustrations of these three new species are provided, and a key for their separation is also given.

  3. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae), parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae) in Mexico

    Science.gov (United States)

    Yefremova, Zoya; González-Santarosa, Graciela; Lomeli-Flores, J. Refugio; Bautista-Martínez, Néstor

    2014-01-01

    Abstract Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae) is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae). Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given. PMID:24478580

  4. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae), parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae) in Mexico.

    Science.gov (United States)

    Yefremova, Zoya; González-Santarosa, Graciela; Lomeli-Flores, J Refugio; Bautista-Martínez, Néstor

    2014-01-01

    Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae) is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae). Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  5. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

    Directory of Open Access Journals (Sweden)

    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  6. A new species in the genus Crisicoccus Ferris (Hemiptera: Coccomorpha: Pseudococcidae), with a key to Chinese species.

    Science.gov (United States)

    Zhang, Jiang-Tao; Wu, San-An

    2016-06-01

    A new mealybug, Crisicoccus ziziphus sp. nov. (Hemiptera: Coccomorpha: Pseudococcidae), collected on the leaves and twigs of Ziziphus jujuba (Rhamnaceae), is described from China. All the female developmental stages (adult, third-instar, second-instar and first-instar nymphs) are described and illustrated. Keys are provided to separate the female instars and to identify adult females of Crisicoccus species from China.

  7. A revision of the tribe Coelidiini of the Oriental, Palearctic and Australian biogeographical regions (Hemiptera: Cicadellidae: Coelidiinae)

    OpenAIRE

    Nielson, Mervin W.

    2016-01-01

    Within the tribe Coelidiini, subfamily Coelidiinae (Cicadellidae: Hemiptera), fragmentation of the genera Calodia Nielson, Olidiana McKamey and Taharana Nielson established the following 13 new genera: Cladolidia, type-species, Lodiana cladopenis Zhang; Creberulidia, type-species, Calodia paucita Nielson; Glaberana, type-species, Glaberana spadix, sp. nov.; Hamusolidia, type-species, Hamusolidia introrsa, sp. nov.; Hiatusorus, typespecies, Taharana schonhorsti Nielson; Laosolidia, type-specie...

  8. Portanus Ball: descrição de uma espécie nova (Hemiptera, Cicadellidae, Xestocephalinae

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    Adenomar Neves de Carvalho

    2004-01-01

    Full Text Available Portanus dubius sp. nov. é descrita e ilustrada a partir de espécimens que foram coletados com armadilha Malaise durante um levantamento entomológico no Estado do Paraná, Brasil (PROFAUPAR.Portanus Ball: description of a new species (Hemiptera, Cicadellidae, Xestocephalinae. Portanus dubius sp. nov. is described and illustrated. The specimens were collected with Malaise trap during an entomological inventory in Paraná State, Brazil (PROFAUPAR.

  9. Oncopsis nigrofaciala sp.nov., a new Macropsinae species (Hemiptera: Cicadellidae) from China%中国横皱叶蝉属一新种记述(半翅目:叶蝉科)

    Institute of Scientific and Technical Information of China (English)

    李虎; 戴仁怀; 李子忠

    2012-01-01

    记述采集山西横皱叶蝉属1新种:黑面横皱叶蝉Oncopsis nigrofaciala sp.nov..新种近似于黑带横皱叶蝉O.nigrofasciata Xu,Liang& Li,2006,但前者颜面大面积黑色,背连索中部的细长突起和阳茎的形状明显不同.%A new leafhopper species,Oncopsis nigrofaciala Li,Dai & Li sp.nov.from Shanxi Province of China,is described and illustrated,and a key to male Oncopsis species known in China is provided.

  10. Egg parasitoids of Megamelus spp. (Hemiptera: Delphacidae in Argentina

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    Serguei V. TRIAPITSYN

    2010-01-01

    Full Text Available Se revisaron los parasitoides oófagos (Hymenoptera: Eulophidae, Mymaridae, y Platygastridae de Megamelus spp. (Hemiptera: Delphacidae de la Argentina, y se presenta una clave para su identificación. Se describen cuatro especies nuevas: Anagrus (Anagrus empanadus Triapitsyn, sp. nov. (Mymaridae, parasitoide de M. scutellaris Berg que se alimenta de camalote, Eichhornia crassipes (Martius Solms-Laubach; Aprostocetus (Ootetrastichus riverplaticus Triapitsyn, sp. nov. (Eulophidae: Tetrastichinae, parasitoide de M. bellicus Marino de Remes Lenicov & Sosa; A. (Ootetrastichus yerbamatei Triapitsyn, sp. nov. (parasitoide de M. bellicus, M. scutellaris y Megamelus sp., todos de la provincia de Buenos Aires (A. (Ootetrastichus yerbamatei también se encuentra en Formosa; y Parascelio sabcli Triapitsyn, sp. nov. (Platygastridae: Scelioninae, parasitoide de M. scutellaris en Formosa, la asociación con el huésped es tentativa. Se incluyen otros parasitoides oófagos conocidos de Megamelus spp. en la Argentina, tales como Kalopolynema (Kalopolynema poema Triapitsyn & Berezovskiy (Mymaridae, parasitoide de M. scutellaris en Buenos Aires y también Cremastobaeus atratus Loiácono & Mulvani (Platygastridae: Scelioninae, parasitoide de M. scutellaris en Formosa, la asociación con el huésped es tentativa.

  11. PARAPORISACCUS GUIZHOUENSIS GEN. ET SP. NOV. OF BAMBOO MEALYBUGFROM CHINA ( HEMIPTERA, COCCOIDEA, PSEUDOCOCCIDAE)%中国竹类粉蚧一新属一新种(半翅目,蚧总科,粉蚧科)

    Institute of Scientific and Technical Information of China (English)

    吕渊; 武三安

    2011-01-01

    A new genus and new spedes on bamboo are described in the mealybug family, Pseudococcidae.The type specimens are deposited in the Insect Collection of Beijing Forestry University.Paraporisacus gen. nov.Type species: Paraporisaccus guizhouensis sp. nov.Body of adult females ovate to obovate, flattened dorso-ventrally, last four abdominal segments well-defined and expanded laterally. Antennae 2 or 3-segmented. Eyes present. Clypeolabral shield with an anterior projection. Prothoracic and mesothoracic legs reduced to unsegmented or segmented stub; daws usually present, without denticles; metathoracic legs represented by bag-like structures with small duct-like pores. Ostioles and circuli absent. Vulva slit-like,directed caudally. Anal ring with pores and 6 setae,located at end of a short invaginated tube. Cerarii absent. Trilocular pores present, distributed over dorsal and ventral surface. Quinqueloculur pores and multilocular pores absent. Oral collar tubular ducts present, short-typed.This new genus is close to the genus Porisaccus Hendricks & Kosztarab, 1999 in bag-like metathoracic legs, but can be easily distinguished by with tubular ducts, prothoracic and mesothoracic legs, and antennae 2 or 3-segmented. The new genus is also similar to Paraserrolecanium Wu, 2010, but differ from the later by 2 or 3 segmented antennae and bag-like metathoracic legs.Paraporisaccus guizhouensis sp. nov. ( Figs 1 - 7 ) Holotype ♀, Guizhou, Suiyang County, Kuan-kuoshui Nature Reserve (28°12′N, 107°8′E), under the leaf sheath of Tushania sp., 12 Aug. 2010, coll. WU San-An and LV Yuan. Paratypes: 18♀ ♀, 15 1st instar nymphs, 2 2nd instar nymphs and 3 3rd instar nymphs, the same data as holotype.Etymology. The genus name Paraporisaccus means this new genus is dose to genus Porisaccis Hendricks & Kosztarab, 1999; the new spedes guizhouensis is named after its type locality ( Guizhou Province).%记述粉蚧科1新属1新种,即拟囊粉蚧属Paraporisaccus gen.nov.及其模

  12. Three new species of Baeoentedon Girault (Hymenoptera: Eulophidae) from China, with the first record of whitefly host association (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Wang, Zhu-Hong; Huang, Jian; Polaszek, Andrew

    2014-07-01

    Three new species of Baeoentedon Girault (Hymenoptera: Eulophidae) are described from China, Baeoentedon balios Wang, Huang & Polaszek sp. nov., Baeoentedon bouceki Wang, Huang & Polaszek sp. nov. and Baeoentedon virgatus Wang, Huang & Polaszek sp. nov. Both female and male of Baeoentedon balios were reared from the whitefly Pealius spina (Singh) (Hemiptera: Aleyrodidae) on the bodhi tree Ficus religiosa L. (Urticales: Moraceae). The male and the whitefly host association of Baeoentedon are recorded for the first time. A key is also provided to females of the world species of the genus.

  13. Gender- and species-specific characteristics of bacteriomes from three psyllid species (Hemiptera: Psylloidae)

    Science.gov (United States)

    Psyllids (Hemiptera: Pyslloidea) harbor bacterial symbionts in specialized organs called bacteriomes. Bacteriomes may be subject to manipulation to control psyllid pests including Bactericera cockerelli (Sulc) (Hemiptera: Triozidae) and Cacopsylla pyricola (Forster) (Hemiptera: Psyllidae) if the bi...

  14. Egg parasitoids of Taosa spp. (Hemiptera: Dictyopharidae in Formosa, Argentina, with descriptions of two new species

    Directory of Open Access Journals (Sweden)

    Serguei V. TRIAPITSYN

    2011-01-01

    Full Text Available Se revisaron los parasitoides oófagos (Hymenoptera: Eulophidae y Platygastridae de Taosa (Cuernavaca longula Remes Lenicov (Hemiptera: Dictyopharidae y se presenta una clave para su identificación. Este dictiofárido se alimenta de camalote, Eichhornia crassipes (Martius Solms-Laubach, y fue colectado en Formosa, Argentina, donde algunos de sus huevos estaban parasitados. Se describen dos especies nuevas: Aprostocetus (Ootetrastichus taosae Triapitsyn, sp. nov. (Eulophidae: Tetrastichinae y Telenomus formosanus Triapitsyn, sp. nov. (Platygastridae: Telenominae. La última especie, que pertenece al grupo crassiclava de especies de Telenomus Haliday, también ataca los huevos de Taosa sp. sobre Pontederia subovata (Seubert Lowden. Estos son los primeros parasitoides oófagos registrados para el género Taosa Distant.

  15. Occurrence of the Tamarix Leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), in Argentina.

    Science.gov (United States)

    Virla, Eduardo G; Logarzo, Guillermo A; Paradell, Susana L

    2010-01-01

    The paleartic tamarix leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), can reduce the growth of tamarisk due to the aggregate feeding imposed by their populations. The species was mentioned for Argentina in Metcalf's catalogue (1967) without locality or region reference, and the contributions on Cicadellidae published by many authors after Metcalf omitted this distributional data. Populations of O. stactogalus on Tamarix sp. were found in 12 sites between 28 degrees 48' to 39 degrees 17' S and 64 degrees 06' to 70 degrees 04' W, located in both the Neotropical and Andean biogeographic regions.

  16. A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae).

    Science.gov (United States)

    Germain, J-F; Matile-Ferrero, D; Kaydan, M B; Malausa, T; Williams, D J

    2015-07-01

    Une nouvelle espèce de Dysmicoccus nuisible à la lavande en Provence (France) (Hemiptera, Sternorrhyncha, Pseudococcidae). Dysmicoccus lavandulae Germain, Matile-Ferrero & Williams n. sp. est décrite et illustrée. Ses séquences ADN sont présentées. L'espèce vit sur Lavandula x intermedia cultivée pour la production d'essence de lavande en Provence. La liste des espèces de pseudococcines vivant sur les lavandes spontanées en France est dressée. Le statut des 2 genres voisins Trionymus Berg et Dysmicoccus Ferris est discuté.

  17. First record of the leafhopper genus Sweta Viraktamath & Dietrich (Hemiptera, Cicadellidae,Typhlocybinae from China, with description of one new species feeding on bamboo

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    Lin Yang

    2012-04-01

    Full Text Available Sweta bambusana sp. n. (Hemiptera: Cicadellidae: Typhlocybinae: Dikraneurini, a new bamboo-feeding species, is described and illustrated from Guizhou and Guangdong of China. This represents the first record of the genus Sweta Viraktamath & Dietrich from China and the second known species of the genus. The new taxon extends the range of the genus Sweta, previously known only from northeast India and Thailand, considerably eastwards. A key for separation of the species of Sweta is given.

  18. The genus Lycoderides Sakakibara, stat. nov., its composition and descriptions of new species (Hemiptera, Membracidae, Stegaspidinae

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    Albino M. Sakakibara

    2013-09-01

    Full Text Available The genus Lycoderides Sakakibara, stat. nov. , its composition and descriptions of new species (Hemiptera, Membracidae, Stegaspidinae.The subgenus Lycoderes (Lycoderides Sakakibara, 1972 is raised to the genus category - Lycoderides stat. nov.. - : and it now includes: Lycoderides amazonicus (Sakakibara, 1991, comb. nov. , Lycoderides brevilobus (Sakakibara, 1972, comb. nov. , Lycoderides burmeisteri (Fairmaire, 1846, comb. nov. , Lycoderides cultratus (Sakakibara, 1991, comb. nov. , Lycoderides fernandezi (Strümpel, 1988, comb. nov. , Lycoderides fuscus (Amyot & Serville, 1843, comb. nov. , Lycoderides gradatus (Sakakibara, 1972, comb. nov. , Lycoderides hippocampus (Fabricius, 1803, comb. nov. , Lycoderides luteus (Funkhouser, 1940, comb. nov. , Lycoderides marginalis (Walker, 1851, comb. nov. , Lycoderides nathanieli (Cryan, 1999, comb. nov. , Lycoderides obtusus (Sakakibara, 1991, comb. nov. , Lycoderides pennyi (Sakakibara, 1991, comb. nov. , Lycoderides phasianus (Fowler, 1896, comb. nov. (= Enchenopa minamen Buckton, 1901,SYN. NOV: , Lycoderides protensus (Sakakibara, 1991, comb. nov. , Lycoderides serraticornis (Fowler, 1896, comb. nov. , and Lycoderides strumpeli (Sakakibara, 1991, comb. nov. The following new species are described: Lycoderides abditus, sp. nov. , Lycoderides brulei,SP. NOV. (: both from French Guiana, Lycoderides capixaba, sp. nov. (from Brazil, Espírito Santo, Lycoderides cavichiolii, sp. nov. (from Brazil, Rio de Janeiro, Lycoderides meloi, sp. nov. (from Brazil, Bahia, and Lycoderides oliviae, sp. nov. (from Brazil, Minas Gerais. Other nomenclatural change: Stegaspis bracteata (Fabricius, 1787 = Lycoderes capitata Buckton, 1903, syn. nov. New records of geographical distribution and a key to the species are provided.

  19. A survey of scale insects in soil samples from Europe (Hemiptera, Coccomorpha).

    Science.gov (United States)

    Kaydan, Mehmet Bora; Benedicty, Zsuzsanna Konczné; Kiss, Balázs; Szita, Éva

    2016-01-01

    In the last decades, several expeditions were organized in Europe by the researchers of the Hungarian Natural History Museum to collect snails, aquatic insects and soil animals (mites, springtails, nematodes, and earthworms). In this study, scale insect (Hemiptera: Coccomorpha) specimens extracted from Hungarian Natural History Museum soil samples (2970 samples in total), all of which were collected using soil and litter sampling devices, and extracted by Berlese funnel, were examined. From these samples, 43 scale insect species (Acanthococcidae 4, Coccidae 2, Micrococcidae 1, Ortheziidae 7, Pseudococcidae 21, Putoidae 1 and Rhizoecidae 7) were found in 16 European countries. In addition, a new species belonging to the family Pseudococcidae, Brevennia larvalis Kaydan, sp. n. and a new species of Ortheziidae, Ortheziola editae Szita & Konczné Benedicty, sp. n. are described and illustrated based on the adult female stage. Revised keys to the adult females of Brevennia and Ortheziola are presented.

  20. Megamelus bellicus (Hemiptera: Delphacidae: immature stages and biology Megamelus bellicus (Hemiptera: Delphacidae: estados inmaduros y biología

    Directory of Open Access Journals (Sweden)

    Roxana Mariani

    2007-12-01

    Full Text Available The immature stages of Megamelus bellicus Remes Lenicov & Sosa (Hemiptera: Delphacidae are described, keyed and illustrated. The description of each stage was based on 24-h hatched nymphs from the laboratory colony. The main characters that distinguish the various stages are: body size, color, number of tarsomeres, espinulation of the metatibia and number of teeth on the spur; presence of sensoria on antennal pedicel. This insect was reared on pieces of water hyacinth, Eichhornia crassipes (Martius Solms Laubach. Biological data based on lab and field observations show that M. bellicus carries out its biological cycle successfully on Pontederiaceae. One to nine eggs per scar are laid deeply into the aerenquima of the petiole, being most frequent 3-4, less frequent 5, 2 and 6 and rarely 1, 7 and 9. Eulophid wasps Aprostocetus (Ootetrastichus sp, known as eggs parasitoid, was registered and quantified. As M. bellicus occupies the same ecological habitat that M. scutellaris Berg, we highlighted some morphological and biological aspects that allow their differentiation.Se describen e ilustran los estados inmaduros de Megamelus bellicus Remes Lenicov & Sosa (Hemiptera: Delphacidae y se presenta una clave para identificarlos. La descripción de cada estadio se realizó sobre la base de ninfas extraidas 24 horas posteriores a la eclosión, de colonias de laboratorio y criadas sobre trozos de hojas de camalote Eichhornia crassipes (Martius Solms Laubach. Los principales caracteres para distinguir los distintos estadios son: tamaño del cuerpo, color, número de tarsómeros, espinulación de la metatibia y número de dientes del calcar y presencia de sensorios en el pedicelo antenal. Datos biológicos basados en observaciones en el laboratorio y en el campo, muestran que M. bellicus realiza su ciclo biológico exitosamente sobre Pontederiaceae. Los huevos, dispuestos de 1 a 9 por postura, son colocados profundamente en el aerénquima del pecíolo; son m

  1. Presencia de Diaphorina citri (Hemiptera: Psyllidae en el Noroeste Argentino (NOA Record of Diaphorina citri (Hemiptera: Psyllidae in North West Argentina

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    Lucrecia Augier

    2006-12-01

    Full Text Available Se amplía la distribución de Diaphorina citri Kuwayama (Hemiptera: Psyllidae para el Noroeste Argentino.The distribution of Diaphorina citri Kuwayama (Hemiptera: Psyllidae is expanded to North West Argentina.

  2. Three new species of mealybug (Hemiptera, Coccomorpha, Pseudococcidae) on persimmon fruit trees (Diospyros kaki) in southern Brazil.

    Science.gov (United States)

    Pacheco da Silva, Vitor C; Kaydan, Mehmet Bora; Germain, Jean-François; Malausa, Thibaut; Botton, Marcos

    2016-01-01

    Brazil has the greatest insect diversity in the world; however, little is known about its scale insect species (Hemiptera: Coccomorpha). Mealybugs (Pseudococcidae) have been found in at least 50% of persimmon orchards Diospyros kaki L. in the southern part of the country. In this study three new mealybug species on persimmon trees located in the Serra Gaúcha Region, RS, Brazil, namely, Anisococcus granarae Pacheco da Silva & Kaydan, sp. n., Ferrisia kaki Kaydan & Pacheco da Silva, sp. n. and Pseudococcus rosangelae Pacheco da Silva & Kaydan, sp. n. are described. In addition, an identification key for the genera occurring on fruit orchards and vineyards in Brazil is provided, together with illustrations and molecular data for the new species.

  3. Description of a new soft scale insect of the genus Pulvinaria Targioni Tozzetti (Hemiptera, Coccoidea, Coccidae) from Bogota, Colombia.

    Science.gov (United States)

    Tanaka, Hirotaka; Kondo, Takumasa

    2015-01-01

    A new soft scale (Hemiptera: Coccoidea: Coccidae) species, Pulvinariacaballeroramosae Tanaka & Kondo, sp. n., is described from specimens collected on twigs of Ficussoatensis Dugand (Moraceae) in Bogota, Colombia. The new species resembles Pulvinariadrymiswinteri Kondo & Gullan, described from Chile on Drimyswinteri J.R. Forst. & G. Forst. (Winteraceae), but differs in the distribution of preopercular pores on the dorsum, the presence of dorsal tubular ducts, dorsal microducts, and reticulation on the anal plates; and in its feeding habits, i.e., Pulvinariacaballeroramosae feeds on the twigs whereas Pulvinariadrymiswinteri feeds on the leaves of its host. A key to the Colombian species of Pulvinaria Targioni Tozzetti is provided.

  4. Jurassic Progonocimicidae (Hemiptera) from China and phylogenetic evolution of Coleorrhyncha

    Institute of Scientific and Technical Information of China (English)

    SZWEDO; Jacek

    2009-01-01

    The fossil record of Progonocimicidae of Hemiptera suborder Coleorrhyncha from China is reviewed. Ovicimex laiyangensis Hong et Wang, 1990, from the Lower Cretaceous of Laiyang, is excluded from Coleorrhyncha. The available generic name Mesocimex Hong, 1983 is resurrected from synonymy and proposed for replacement of the preoccupied name and junior homonym Mesoscytina Hong, 1983, non Mesoscytina Tillyard, 1919. It resulted in following new combinations: Mesocimex abditus (Yu. Popov, 1982) comb. n., Mesocimex ambiguus (Yu. Popov, 1985) comb. n., Mesocimex brunneus (Hong, 1983), comb. n., Mesocimex fidus (Yu. Popov, 1982) comb. n., Mesocimex intermedius (Yu. Popov, 1985) comb. n., Mesocimex kuzbasicus (Yu. Popov, 1985) comb. n., Mesocimex liliputus (Yu. Popov, 1988) comb. n., Mesocimex minutus (Yu. Popov, 1982) comb. n., Mesocimex modestus (Yu. Popov, 1985) comb. n., Mesocimex paulinus (Yu. Popov, 1982) comb. n., Mesocimex cognatus (Yu. Popov, 1982) comb. n. An annotated list of species of Mesocimex is given. In addition, a new species, Mesocimex lini sp. nov., is described based on well-preserved specimens with wings and bodies from the Middle Jurassic of Daohugou, China. This discovery supports the Middle Jurassic age of Daohugou Lagerstatte, since all species of Mesocimex are confined to the Lower-Middle Jurassic. The phylogenetic evolution of Coleorrhyncha is discussed: Cicadocorinae, Karabasiinae, Hoploridiinae, and Peloridiidae are monophyletic clades whereas Progonocimicinae and Karabasidae are clearly paraphyletic groups.

  5. Systematics and phylogeny of the Neotropical treehopper subfamily Nicomiinae (Hemiptera, Membracidae Sistemática e filogenia das cigarrinhas neotropicais da subfamília Nicomiinae (Hemiptera, Membracidae

    Directory of Open Access Journals (Sweden)

    Jesse L. Albertson

    2005-03-01

    Full Text Available Morphological characters of adults of the treehopper subfamily Nicomiinae Haupt, 1929 (Hemiptera, Membracidae including seven genera (Eudonica gen. nov.; Euwalkeria Goding, 1926; Holdgatiella Evans, 1962; Nicomia Stål, 1858; Nodonica Dietrich, McKamey& Deitz, 2001; Stalomia gen. nov.; and Tolania Stål, 1858 and 22 species (16 new are described and illustrated. Keys are provided for genera and for species of Euwalkeria, Holdgatiella, and Nicomia. Nomenclatural changes, based on study of the primary type material of 15 species, include three new combinations, one new synonymy, and reinstatement of one junior synonym. Eudonica has one species, Eudonica nanella sp. nov.; Euwalkeria has five species, including four new species: E. colorata sp. nov., E. distincta sp. nov., E. perdita sp. nov., E. rubrica sp. nov.; Holdgatiella has two species, one of which is described as new: Holdgatiella chiloensis sp. nov.; Nicomia has twelve species, nine of which are described as new: N. buccina sp. nov., N. harenosa sp. nov., N. inscripta sp. nov., N. jucunda sp. nov., N. monticola sp. nov., N. nigrifasciata sp. nov., N. notidana sp. nov., N. pulchella sp. nov., N. serrata sp. nov.; Nodonica has one species, Nodonica bispinigera Dietrich, McKamey & Deitz; and Stalomia has one species, Stalomia veruta sp. nov. Tolania contains eleven previously described species and nearly 60 new species, which will be treated in a later publication. Three new combinations are proposed: one species described in Nicomia is placed in the tribe Abelini (Centrotinae as Abelus retrospinosus (Lethierry comb. nov.; one species previously placed in Nicomia is transferred to the genus Tolania as T. obliqua (Walker, 1858, comb. nov.; one species described in Holdgatiella is placed in the genus Tolania as T. stria (Cryan & Deitz, 2002, comb. nov. One new synonymy is proposed: Hoplophera [sic] cicadoides Walker, 1862, syn. nov., a junior synonym of Nicomia interrupta Stål, 1858. Nicomia

  6. A new iranian species of Uroleucon Mordvilko, 1914 (Hemiptera, Aphididae from specimens in the Natural History Museum collection

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    Nieto Nafría, Juan M.

    2014-06-01

    Full Text Available Uroleucon (Uromelan helichrysi sp. n. (Hemiptera, Aphididae, Aphidinae: Macrosiphini is established from Iranian apterous and alate viviparous females caught on Helichrysum sp. (Asteraceae and preserved in the collection of the Natural History Museum (BMNH in London. The number of setae on the first tarsal segments (5, the number of caudal setae (20 to 28, the presence of abdominal marginal tubercles on abdominal segments 2 to 4, and the size of the cells of the siphuncular reticulation (relatively small allow the new species to be distinguished from other Palaeartic species of the subgenus Uromelan. Blackman and Eastop’s key to apterae on Helichrysum is modified to include the new species. urn:lsid:zoobank.org:pub:E2B89D59-05FD-4A9E-AD78-B3C302219A10Se establece la especie Uroleucon helichrysi n. sp. (Hemiptera: Aphididae: Aphidinae: Macrosiphini con la descripción de sus hembras vivíparas ápteras y aladas recogidas en Irán sobre Helichrysum sp. (Asteraceae, conservadas en la colección del Natural History Museum de Londres. La nueva especie se diferencia de otras especies paleárticas del subgénero Uromelan por la cantidad de setas en el primer segmento tarsal (5, la cantidad de setas caudales (20 a 28, por el tamaño de las celdillas de la reticulación cornicular (que son relativamente pequeñas y por la presencia de papilas marginales (presentes en los segmentos abdominales 2 a 4 Se incluye una modificación a la clave de Blackman and Eastop para las ápteras que viven sobre Helichrysum. urn:lsid:zoobank.org:pub:E2B89D59-05FD-4A9E-AD78-B3C302219A10

  7. Occurrence of the parasitoid Anastatus sp. in eggs of Leptoglossus zonatus under the maize in Brazil Ocorrência do parasitóide Anastatus sp. em ovos de Leptoglossus zonatus em milho no Brasil

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    Carlos Henrique Marchiori

    2003-08-01

    Full Text Available The objective of this study was to report, for the first time in Brazil, the occurrence of the parasitoid Anastatus sp. (Hymenoptera: Eupelmidae in eggs of Leptoglossus zonatus (Hemiptera: Coreidae on maize (Zea mays L. in Itumbiara County, State of Goias, Brazil (18°25'S; 49°13'W. Percent parasitism was 6.9%.O objetivo dessa nota é registrar a primeira ocorrência, no Brasil, do parasitóide Anastatus sp. (Hymenoptera: Eupelmidae em ovos de Leptoglossus zonatus (Hemiptera: Coreidae em cultivar de milho em Itumbiara, Estado de Goiás (18°25'S; 49°13'W. A porcentagem de parasitismo foi de 6,9%.

  8. Biology and systematics of gall-inducing triozids (Hemiptera: Psylloidea) associated with Psidium spp. (Myrtaceae).

    Science.gov (United States)

    Carneiro, René G S; Burckhardt, Daniel; Isaias, Rosy M S

    2013-01-01

    Psidium myrtoides (Myrtaceae) shelters the gall inducer Nothotrioza myrtoidis gen. et sp. n. (Hemiptera: Psylloidea) which is described and illustrated here. Nothotrioza belongs to the family Triozidae and is probably most closely related to Neolithus, a monotypic Neotropical genus associated with Sapiun (Euphorbiaceae). Three species are recognized within Nothotrioza: the type species N. myrtoidis sp. n. associated with Psidium myrtoides, N. cattleiani sp. n. (misidentified by Butignol & Pedrosa-Macedo as Neotrioza tavaresi) with Psidium cattleianum, and N. tavaresi (Crawford) comb. n. (from Neotrioza) with an unidentified species of Malpighiaceae, respectively. A lectotype is designated here for Neotrioza tavaresi. Also, the diversity of insect galls associated with P. myrtoides and the biology of N. myrtoidis were examined. N. myrtoidis presents five instars and an annual life cycle synchronised with the phenology of P. myrtoides. Gall size was proportional to the insect developmental stages, and rates of parasitism and mortality were 15.7 % and 29.8 %, respectively. The red colour is an important macroscopic diagnostic feature of the gall that could be associated with parasite-free condition of the galling insect. The biological features presented by the system Psidium myrtoides--Nothotrioza myrtoidis are in accordance with other systems involving sucking galling insects, however, it is exceptional by its univoltine life cycle associated with a perennial plant in the Neotropics. The galls induced by the three known Nothotrioza spp. are morphologically similar, i.e. closed, globoid and unilocular, as well as the opening mechanism for releasing the adults.

  9. Seasonal variation of Hemiptera community of a temple pond of Cachar District, Assam, northeastern India

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    K. Das

    2012-09-01

    Full Text Available The study records seven families, 11 genera and 14 species of hemipteran insect community in different seasons in a temple pond near Silchar, Cachar District, Assam, northeastern India. The pond is very rich in macrophytes like Nelumbo nucifera (Water Lotus, Hygrorhiza aristata (Indian Lotus, Cynodon dactylon (Bermuda Grass, Philotria sp. etc. The hemipteran families recorded in the system were Corixidae, Gerridae, Aphididae, Mesoveliidae, Notonectidae, Nepidae and Belostomatidae. The species were Micronecta haliploides, Micronecta (Basileonecta scutellaris scutellaris (Stål (Corixidae; Neogerris parvula (Stål, Limnogonus nitidus (Mayr, Tenagogerris sp., Rhagadotarsus sp. (Gerridae; Enithares ciliata (Fabricius, Anisops lundbladiana Landsbury, (Notonectidae; Diplonychus rusticus (Fabricius and Diplonychus annulatus (Fabricius (Belostomatidae, Rhopalosiphum nymphaeae (Linnaeus (Aphididae, Ranatra elongata (Fabricius, Ranatra varipes varipes (Stål (Nepidae and Mesovelia vittigera Horváth (Mesoveliidae. The highest population of Hemiptera was recorded during the post-monsoon followed by the pre-monsoon and the monsoon periods. The lowest was recorded in the winter. Shannon Weiner diversity index (H/ and evenness index (J/ showed the highest diversity and evenness during the post monsoon period. Berger Parker index of dominance (d was found highest in winter. In winter both diversity and density were the lowest. The study revealed the presence of four dominant species and three sub-dominant species in the pond. Insect diversity did not show any significant relationship with the environmental variables.

  10. A new mealybug in the genus Pseudococcus Westwood (Hemiptera: Coccomorpha: Pseudococcidae) from North America, with a key to species of Pseudococcus from the New World.

    Science.gov (United States)

    Ellenrieder, Natalia Von; Watson, Gillian

    2016-04-19

    A mealybug species that feeds on Agave spp., Pseudococcus variabilis sp. n. (Hemiptera: Coccomorpha: Pseudococcidae), is described from North America. Its entry into the United States was likely via the horticultural trade on its host plants in the genus Agave (Liliales: Agavaceae). Descriptions and illustrations of the adult female and male, diagnosis from congeners in the New World, and a molecular characterization based on COI are provided, as well as a key to adult females of all Pseudococcus species recorded from the New World.

  11. A molecular phylogeny of Hemiptera inferred from mitochondrial genome sequences.

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    Nan Song

    Full Text Available Classically, Hemiptera is comprised of two suborders: Homoptera and Heteroptera. Homoptera includes Cicadomorpha, Fulgoromorpha and Sternorrhyncha. However, according to previous molecular phylogenetic studies based on 18S rDNA, Fulgoromorpha has a closer relationship to Heteroptera than to other hemipterans, leaving Homoptera as paraphyletic. Therefore, the position of Fulgoromorpha is important for studying phylogenetic structure of Hemiptera. We inferred the evolutionary affiliations of twenty-five superfamilies of Hemiptera using mitochondrial protein-coding genes and rRNAs. We sequenced three mitogenomes, from Pyrops candelaria, Lycorma delicatula and Ricania marginalis, representing two additional families in Fulgoromorpha. Pyrops and Lycorma are representatives of an additional major family Fulgoridae in Fulgoromorpha, whereas Ricania is a second representative of the highly derived clade Ricaniidae. The organization and size of these mitogenomes are similar to those of the sequenced fulgoroid species. Our consensus phylogeny of Hemiptera largely supported the relationships (((Fulgoromorpha,Sternorrhyncha,Cicadomorpha,Heteroptera, and thus supported the classic phylogeny of Hemiptera. Selection of optimal evolutionary models (exclusion and inclusion of two rRNA genes or of third codon positions of protein-coding genes demonstrated that rapidly evolving and saturated sites should be removed from the analyses.

  12. A taxonomic monograph of the assassin bug genus Zelus Fabricius (Hemiptera: Reduviidae): 71 species based on 10,000 specimens.

    Science.gov (United States)

    Zhang, Guanyang; Hart, Elwood R; Weirauch, Christiane

    2016-01-01

    The New World assassin bug genus Zelus Fabricius, 1803 (Insecta: Hemiptera: Heteroptera: Reduviidae: Harpactorinae: Harpactorini) is revised based on more than 10,000 specimens. Seventy-one species are recognized and twenty-four described as new: Zelus aithaleos sp. n., Zelus amblycephalus sp. n., Zelus antiguensis sp. n., Zelus auralanus sp. n., Zelus bahiaensis sp. n., Zelus banksi sp. n., Zelus casii sp. n., Zelus championi sp. n., Zelus cordazulus sp. n., Zelus fuliginatus sp. n., Zelus gilboventris sp. n., Zelus gracilipes sp. n., Zelus grandoculus sp. n., Zelus kartaboides sp. n., Zelus lewisi sp. n., Zelus panamensis sp. n., Zelus paracephalus sp. n., Zelus rosulentus sp. n., Zelus russulumus sp. n., Zelus spatulosus sp. n., Zelus truxali sp. n., Zelus umbraculoides sp. n., Zelus umbraculus sp. n., and Zelus xouthos sp. n. Five species, Zelus araneiformis Haviland, 1931, Zelus gradarius Bergroth, 1905, Zelus modestus (Stål, 1862), Zelus subfasciatus Stål, 1860 and Zelus vittaticeps Stål, 1866, are removed from Zelus and placed incertae sedis within Harpactorini. Nine new synonyms are recognized (senior synonym in parentheses): Zelus atripes Champion, 1898 syn. nov. (=Zelus conjungens [Stål, 1860]), Zelus dispar Fabricius, 1803 syn. nov. (=Zelus pedestris Fabricius, 1803), Zelus formosus Haviland, 1931 syn. nov. (=Zelus laticornis Herrich-Schaeffer, 1853), Zelus obscuridorsis (Stål, 1860) syn. nov. (=Zelus pedestris), Zelus pallidinervus Haviland, 1931 syn. nov. (=Zelus kartabensis Haviland, 1931), Zelus personatus Berg, 1879 syn. nov. (=Zelus versicolor Herrich-Schaeffer, 1848), Zelus trimaculatus Champion, 1898 syn. nov. (=Zelus means Fabricius, 1803), Zelus trimaculicollis (Stål, 1855) syn. nov. (=Zelus means), and Zelus tristis Haviland, 1931 syn. nov. (=Zelus laticornis). Zelus conjungens (Stål, 1860) stat. rev. Is resurrected from junior synonymy with zealous armillatus (Lepeletier & Seville, 1825). Zelus ambulans Stål, 1862 stat. rev. and Zelus

  13. Two new species of Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoids of Hypogeococcus spp. (Hemiptera: Pseudococcidae), with taxonomic notes on some congeneric taxa.

    Science.gov (United States)

    Triapitsyn, Serguei V; Logarzo, Guillermo A; Aguirre, María B; Aquino, Daniel A

    2014-09-15

    Two new species of Anagyrus Howard (Hymenoptera: Encyrtidae) are described from Argentina, A. cachamai Triapitsyn, Logarzo & Aguirre sp. n. (Catamarca, Córdoba, Salta and Tucumán Provinces) and A. quilmes Triapitsyn, Logarzo & Aguirre sp. n. (Catamarca, Salta and Tucumán). Both new species are parasitoids of Hypogeococcus spp. (Hemiptera: Pseudococcidae). Anagyrus cachamai is a parasitoid of H. pungens Granara de Willink on Alternanthera paronychioides, A. pungens and Gomphrena sp. (Amaranthaceae), and also of a Hypogeococcus sp. on Cleistocactus baumannii and Hypogeococcus sp. on C. smaragdiflorus (Cactaceae). Anagyrus quilmes is a parasitoid of H. pungens on A. paronychioides, A. pungens and Gomphrena sp. Other biological traits of the new species are also reported. These parasitoids may be of importance as potential candidate biological control agents against a Hypogeococcus sp., commonly called the Harrisia cactus mealybug and identified as H. pungens, but possibly not belonging to that species. This mealybug threatens the native cacti in some Caribbean islands and Florida, USA, and is devastating the native columnar cacti in Puerto Rico. Illustrations and taxonomic notes on the type specimens of some other, little known described species of Anagyrus from Argentina and Chile are provided, and a key to females of the 14 species of Anagyrus known from Argentina is given. Anagyrus nigriceps (De Santis) syn. n. is synonymized under A. bellator (De Santis). Lectotypes are designated for Paranusia bifasciata Brèthes, Philoponectroma pectinatum Brèthes, and Protanagyrus aciculatus Blanchard. 

  14. Susceptibility of twolined spittlebug (Hemiptera: Cercopidae) life stages to entomophagous arthropods in turfgrass.

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    Nachappa, Punya; Guillebeau, L P; Braman, S K; All, J N

    2006-10-01

    Prosapia bicincta (Say) (Hemiptera: Cercopidae), the twolined spittlebug, is an economic pest of turfgrass in the southeastern United States. No data concerning natural enemies of P. bicincta in turfgrass have been reported previously. We compared predation of spittlebug eggs, nymphs, and adults in the laboratory by potential generalist predators commonly found in turfgrass: bigeyed bugs Geocoris uliginosus Say and Geocoris punctipes Say; red imported fire ant, Solenopsis invicta Buren; wolf spiders (Lycosa sp. Walckenaer); carabid beetles Harpalaus pensylvanicus DeGeer and Calosoma sayi Dejean; and tiger beetles Megacephala carolina carolina L. Eggs were readily consumed by generalist predators. S. invicta consumed 100% of the eggs offered. H. pensylvanicus and C. sayi were also significant predators of P. bicincta eggs. Nymphs live in spittlemasses that protect them from attack by predators, but exposed nymphs were susceptible to attack when mechanically removed from their spittlemasses. S. invicta and M. carolina carolina caused significant mortality of exposed nymphs. P. bicincta adults are aposematic and have the ability to reflex bleed; however, reflex bleeding did not prevent attack by predators. S. invicta and M. carolina carolina killed 100% of the adult spittlebugs offered in laboratory bioassays. Lycosa sp. are less voracious predators of adults. Sound background knowledge about P. bicincta and its potential natural enemy complex is important for the development and implementation of a detailed, site-specific, biologically based pest management program in turfgrass.

  15. Rapid molecular identification of armored scale insects (Hemiptera: Diaspididae) on Mexican 'Hass' avocado.

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    Rugman-Jones, Paul F; Morse, Joseph G; Stouthamer, Richard

    2009-10-01

    'Hass' avocado, Persea americana Mill., fruit imported into California from Mexico are infested with high levels of armored scale insects (Hemiptera: Diaspididae), constituting several species. The paucity and delicate nature of morphological characters traditionally used to diagnose armored scales often require careful preparation of slide-mounted specimens and expert knowledge of the group, for their accurate identification. Here, we present a simple, quick, and accurate means to identify armored scales on Mexican avocados, based on amplification of the internal transcribed spacer two of ribosomal DNA, by using the polymerase chain reaction (PCR). This region seems to show a high level of intraspecific conformity among scale specimens originating from different localities. A suite of species-specific reverse PCR primers are combined in a single reaction, with a universal forward primer, and produce a PCR product of a unique size, that after standard gel electrophoresis, allows the direct diagnosis of six diaspidid species: Abgrallaspis aguacatae Evans, Watson & Miller; Hemiberlesia lataniae (Signoret); Hemiberlesia sp. near latania; Hemiberlesia rapax (Comstock); Acutaspis albopicta (Cockerell); and Pinnaspis strachani (Cooley). Two additional species, Diaspis miranda (Cockerell) and Diaspis sp. near miranda, also are separated from the others by using this method and are subsequently diagnosed by secondary digestion of the PCR product with the restriction endonuclease smaI.

  16. 角冠叶蝉属分类研究(半翅目,叶蝉科,殃叶蝉亚科)%A TAXONOMIC STUDY ON THE GENUS VIRIDOMARUS DISTANT (HEMIPTERA,CICADELLIDAE, EUSCELINAE)

    Institute of Scientific and Technical Information of China (English)

    邢济春; 戴仁怀; 李子忠

    2009-01-01

    In the present paper, the genus Viridomarus Distant, 1918 (Hemiptera, Cicadellidae, Euscelinae) is redescribed, and two new spedes Viridomarus brevialatus sp. nov. and Viridomarus sp. nov. are described and illustrated from Hainan Province, China. A key is given to distinguish all species of the genus. The type specimens of the new species are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) and Institute of Entomology, Guizhou University (IEGU).%对角冠叶蝉属属征作了重新描述,编制了分种检索表,记述该属2新种:短翅角冠叶蝉Viridomarus brevialatus sp.nov.和宽室角冠叶蝉Viridomarus laticellus sp.nov..新种模式标本分别保存在中国科学院动物研究所(IZCAS)和贵州大学昆虫研究所(IEGU).

  17. Description of a new soft scale insect of the genus Pulvinaria Targioni Tozzetti (Hemiptera, Coccoidea, Coccidae from Bogota, Colombia

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    Hirotaka Tanaka

    2015-03-01

    Full Text Available A new soft scale (Hemiptera: Coccoidea: Coccidae species, Pulvinaria caballeroramosae Tanaka & Kondo, sp. n., is described from specimens collected on twigs of Ficus soatensis Dugand (Moraceae in Bogota, Colombia. The new species resembles P. drymiswinteri Kondo & Gullan, described from Chile on Drimys winteri J.R. Forst. & G. Forst. (Winteraceae, but differs in the distribution of preopercular pores on the dorsum, the presence of dorsal tubular ducts, dorsal microducts, and reticulation on the anal plates; and in its feeding habits, i.e., P. caballeroramosae feeds on the twigs whereas P. drymiswinteri feeds on the leaves of its host. A key to the Colombian species of Pulvinaria Targioni Tozzetti is provided.

  18. Evaluation of efficacy of 18 strains of entomopathogenic nematodes (Rhabditida) against Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) under laboratory conditions.

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    Barbosa Negrisoli, Carla Ruth de Carvalho; Negrisoli Júnior, Aldomario Santo; Botton, Marcos; Garcia, Mauro Silveira; Bernardi, Daniel

    2013-07-01

    Planococcus citri (Risso, 1813) (Hemiptera: Pseudococcidae) is an important plant virus vector in grapevine crops in Brazil and other countries. The mealybug grows in roots and leaves of the grapes. Entomopathogenic nematodes (EPNs) are efficient control agents against insects associated to the soil and could be applied with the same equipment used for chemical insecticides. The aim of this study was to select effective EPNs for controlling P. citri females in laboratory conditions (25±1°C, UR 60±10%). We tested 17 native [Steinernema rarum (6 strains), Steinernema glaseri, Steinernema feltiae, Steinernema riobrave, Steinernema sp., Heterorhabditis bacteriophora (7 strains)] and only one exotic strain (Steinernema carpocapsae ALL). The bioassays were done on Petri dishes infested with females of P. citri, which were sprayed with EPNs juveniles. The strain with larger pathogenicity and virulence in laboratory was H. bacteriophora RS33 (from 69.0% to 92.2% of mortality), native of Rio Grande do Sul.

  19. Description of a new species of Anagyrus Howard (Hymenoptera: Chalcidoidea: Encyrtidae), a promising biological control agent of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Sternorrhyncha: Pseudococcidae).

    Science.gov (United States)

    Rameshkumar, A; Noyes, J S; Poorani, J; Chong, J H

    2013-01-01

    Anagyrus amnestos sp. n. (Hymenoptera: Encyrtidae), a promising parasitoid of the invasive Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), is described based on material collected from India. This parasitoid was identified as Anagyrus sp. nov. nr. sinope Noyes & Menezes in recent literature, and was initially collected in Georgia, USA. It was found to be a specific parasitoid of the Madeira mealybug and its biological attributes and potential as a biological control agent of this pest were studied. In what appears to be a case of fortuitous introduction, we detected this parasitoid in large numbers on Madeira mealybugs from the southern Indian state of Karnataka, where the mealybug is a recently introduced invasive pest. In view of its economic importance as a potential biological control agent of the Madeira mealybug, it is formally described and illustrated here. Comparative accounts of the new species vis-a-vis its close relatives in India and the Americas are provided.

  20. Essential oils as fumigants for bed bugs (Hemiptera: Cimicidae)

    Science.gov (United States)

    In Petri dish assays, fumigation of a pyrethroid-susceptible strain of bed bugs Cimex lectularius L. (Hemiptera: Cimicidae) with various essential oils resulted in mortality that approached or equaled 100%, after 5 days. However, when bed bugs were exposed to the same essential oils in sealed, comme...

  1. Release and establishment of Megamelus scutellaris (Hemiptera: Delphacidae) in Florida

    Science.gov (United States)

    Megamelus scutellaris (Berg) (Hemiptera: Delphacidae) was recently developed as a classical biological control agent for waterhyacinth, Eichhornia crassipes Mart. Solms, and released in Florida. Releases were conducted at 10 sites around the state every 4-6 weeks until late fall then halted until t...

  2. The complete mitochondrial genome sequence of Diaphorina citri (Hemiptera: Psyllidae)

    Science.gov (United States)

    The first complete mitochondrial genome (mitogenome) sequence of Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae), from Guangzhou, China is presented. The circular mitogenome is 14,996 bp in length with an A+T content of 74.5%, and contains 13 protein-coding genes (PCGs), 22 tRNA genes ...

  3. Catalog of the adelgids of the world (Hemiptera: Adelgidae)

    Science.gov (United States)

    A taxonomic and nomenclatural catalog of the adelgids (Hemiptera: Adelgidae) is presented. Six family-group names are listed, five being synonyms of Adelgidae. Twenty-two genus-group names, of which nine are valid and in use, are presented with their type species, etymology, and grammatical gender. ...

  4. Developing resistance for watermelon against whiteflies (Hemiptera: Aleyrodidae)

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    Host plant resistance is a fundamental component of crop sustainability. The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a key pest of many crops around the world. It is adaptive to its environment and feeds on an impressive (over 1,000) number of plant species. Watermelon (Cit...

  5. KEBERADAAN DYSMICOCCUS BREVIPES (COCKERELL (HEMIPTERA: PSEUDOCOCCIDAE SEBAGAI VEKTOR PINEAPPLE MEALYBUG WILT-ASSOCIATED VIRUS (PMWAV PADA TANAMAN NANAS

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    Dewi Sartiami

    2006-04-01

    Full Text Available Mealybug can almost be found in all pineapple fields (Ananas comosus (Linnaeus. The insect is known to be a vector of Pineapple Mealybug wilt-associated Virus (PMWaV. The insect samples taken from pineapple in Bunihayu, Jalancagak, Subang, West Java, were identified in laboratory. Mealybug-ant symbionts were also taken. The ability of this ant to carry the mealybugs from colony reared on kabocha (Cucurbita maxima to pineapple was also tested at green house level. Only one spesies of mealybug was found on pineapple, i.e. Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae. The mealybugs were found to colonize root, basal of stem and the leaf. Eight ant species were found to be associated with mealybug. There are four species belongs to Pseudolasius genera, two species Cardiocondyla genera, Paratrechina sp. and Dorylus sp. Paratrechina sp. showed the ability to carry D. brevipes from kabocha population to pineapple. Therefore the ants should also be controlled in the total management of PMWav.

  6. SP. Pescado

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    Renato Gendre

    2003-12-01

    Full Text Available Nell'occhiello di un articolo dal titolo Il Peru dei de[Jini rosa e de/la grande pioggia si legge: "da una partenza  in aereo al «pescado»  che ti  sfamera."1 Questa parola spagnola, giustamente chiusa tra caporali, a noi pare molto interes­ sante, perche, nonostante l'apparenza, non ha nulla da spartire sotto i1 profilo se­ mantico con l'it. pescato. lnfatti, tutti i piu importanti dizionari della lingua italiana, di ieri e di oggi, etimologici e non 2, registrano  accanto a pescata,  ii lemma pescato, 3 ma lo spiegano come "quantita di pesce catturato nel corso di una battuta o di una stagione di pesca",4 mentre lo sp. pescado  indica i1 "pesce (solo nel senso di: pesGe pescato da mangiare [...]".s

  7. DNA barcodes for Nearctic Auchenorrhyncha (Insecta: Hemiptera.

    Directory of Open Access Journals (Sweden)

    Robert G Foottit

    Full Text Available BACKGROUND: Many studies have shown the suitability of sequence variation in the 5' region of the mitochondrial cytochrome c oxidase I (COI gene as a DNA barcode for the identification of species in a wide range of animal groups. We examined 471 species in 147 genera of Hemiptera: Auchenorrhyncha drawn from specimens in the Canadian National Collection of Insects to assess the effectiveness of DNA barcoding in this group. METHODOLOGY/PRINCIPAL FINDINGS: Analysis of the COI gene revealed less than 2% intra-specific divergence in 93% of the taxa examined, while minimum interspecific distances exceeded 2% in 70% of congeneric species pairs. Although most species are characterized by a distinct sequence cluster, sequences for members of many groups of closely related species either shared sequences or showed close similarity, with 25% of species separated from their nearest neighbor by less than 1%. CONCLUSIONS/SIGNIFICANCE: This study, although preliminary, provides DNA barcodes for about 8% of the species of this hemipteran suborder found in North America north of Mexico. Barcodes can enable the identification of many species of Auchenorrhyncha, but members of some species groups cannot be discriminated. Future use of DNA barcodes in regulatory, pest management, and environmental applications will be possible as the barcode library for Auchenorrhyncha expands to include more species and broader geographic coverage.

  8. First record of the leafhopper genus Varicopsella Hamilton, 1980 (Hemiptera: Cicadellidae: Macropsinae) in China, with descriptions of a new subgenus and new species, a checklist, and a key to species.

    Science.gov (United States)

    Li, Hu; Dai, Ren-Huai; Li, Zi-Zhong

    2014-01-01

    A new monobasic leafhopper subgenus, Varicopsella (Multispinulosa) Li, Dai, and Li, subgen. nov., of the subfamily Macropsinae (Hemiptera: Auchenorrhyncha: Membracoidea: Cicadellidae) is proposed to accommodate Varicopsella (Multispinulosa) hamiltoni Li, Dai, and Li, sp. nov.from Guangxi province of China. The new subgenus and new species are described and illustrated. They can be distinguished mainly by characteristics of the fore wings with two anteapical cells; weak dorsoventrally flattened body; aedeagal shaft with paired apical processes on ventral margin; and the shape of the dorsal connective. An updated checklist and an illustrated key for identification of the species of Varicopsella along with geographical distributions of the species are given.

  9. Endemism analysis of Neotropical Pentatomidae (Hemiptera, Heteroptera

    Directory of Open Access Journals (Sweden)

    Augusto Ferrari

    2010-12-01

    Full Text Available The definition of areas of endemism is central to studies of historical biogeography, and their interrelationships are fundamental questions. Consistent hypotheses for the evolution of Pentatomidae in the Neotropical region depend on the accuracy of the units employed in the analyses, which in the case of studies of historical biogeography, may be areas of endemism. In this study, the distribution patterns of 222 species, belonging to 14 Pentatomidae (Hemiptera genera, predominantly neotropical, were studied with the Analysis of Endemicity (NDM to identify possible areas of endemism and to correlate them to previously delimited areas. The search by areas of endemism was carried out using grid-cell units of 2.5° and 5° latitude-longitude. The analysis based on groupings of grid-cells of 2.5° of latitude-longitude allowed the identification of 51 areas of endemism, the consensus of these areas resulted in four clusters of grid-cells. The second analysis, with grid-cells units of 5° latitude-longitude, resulted in 109 areas of endemism. The flexible consensus employed resulted in 17 areas of endemism. The analyses were sensitive to the identification of areas of endemism in different scales in the Atlantic Forest. The Amazonian region was identified as a single area in the area of consensus, and its southeastern portion shares elements with the Chacoan and Paraná subregions. The distribution data of the taxa studied, with different units of analysis, did not allow the identification of individual areas of endemism for the Cerrado and Caatinga. The areas of endemism identified here should be seen as primary biogeographic hypotheses.

  10. An overview on the ecology of Triatominae (Hemiptera:Reduviidae).

    Science.gov (United States)

    Galvão, Cleber; Justi, Silvia A

    2015-11-01

    Chagas disease, the American trypanosomiasis, is an important neglected tropical illness caused by the flagellate protozoan Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) and transmitted by insects of the subfamily Triatominae (Hemiptera: Reduviidae). Here we provide an overview on the current knowledge about Triatominae ecology, its association with human, T. cruzi infection and the immediate consequences of habitat fragmentation. We also discuss the geographic distribution of the species and the importance of predicting their distributions to control programs.

  11. Catalog of the adelgids of the world (Hemiptera, Adelgidae).

    Science.gov (United States)

    Favret, Colin; Havill, Nathan P; Miller, Gary L; Sano, Masakazu; Victor, Benjamin

    2015-01-01

    A taxonomic and nomenclatural Catalogue of the adelgids (Hemiptera: Adelgidae) is presented. Six family-group names are listed, five being synonyms of Adelgidae. Twenty-two genus-group names, of which nine are subjectively valid and in use, are presented with their type species, etymology, and grammatical gender. One hundred and six species-group names are listed, of which 70 are considered subjectively valid.

  12. Taperinha Linnavuori (Hemiptera, Cicadellidae, Deltocephalinae: descrições de nove espécies novas Taperinha Linnavuori (Hemiptera, Cicadellidae, Deltocephalinae: descriptions of nine new species

    Directory of Open Access Journals (Sweden)

    Keti Maria Rocha Zanol

    2004-06-01

    Full Text Available As seguintes novas espécies são descritas e ilustradas: Taperinha manauara sp. nov., Taperinha concinna sp. nov., Taperinha kijaua sp. nov., Taperinha compta sp. nov., Taperinha tefeigua sp. nov., Taperinha assurinia sp. nov., Taperinha pupejua sp. nov., Taperinha freytagi sp. nov. e Taperinha ourensis sp. nov., da Amazônia Brasileira.The following new species are described and illustrated: Taperinha manauara sp. nov., Taperinha concinna sp. nov., Taperinha kijaua sp. nov., Taperinha compta sp. nov., Taperinha tefeigua sp. nov., Taperinha assurinia sp. nov., Taperinha pupejua sp. nov., Taperinha freytagi sp. nov. and Taperinha ourensis sp. nov., from Brazilian Amazonia.

  13. The effects of host, geographic origin, and gender on the thermal requirements of Diaphorina citri (Hemiptera: Psyllidae).

    Science.gov (United States)

    Nava, Dori E; Gomez-Torres, Mariuxi L; Rodrigues, Marjorie D; Bento, José M S; Haddad, Marinéia L; Parra, José R P

    2010-04-01

    Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. We examined how host, geographic region, and gender affect the thermal requirements of D. citri. The insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees C, 70 +/- 10% RH, and a 14 h photophase. Host plants for D. citri included orange (Citrus sinensis [Rutaceae]) varieties Pêra and Natal, the rootstock, Rungpur lime (C. limonia [Rutaceae]) and the natural host, Orange jessamine (Murraya paniculata [Rutaceae]). To study the influence of geographic origin on thermal requirements, we studied D. citri populations from Piracicaba, SP (warmer region) and Itapetininga, SP (cooler region). The duration and survival of the development stages and the duration of the total development (egg-adult) did not differ significantly on the different hosts, but it did vary with temperature. Nymphs of D. citri created on the different hosts have the same thermal requirements. The thermal requirements for this species collected from the two climate regions were identical; males and females also had the same thermal requirements.

  14. The complete mitochondrial genome of a spiraling whitefly, Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Ming-Xing, Lu; Zhi-Teng, Chen; Wei-Wei, Yu; Yu-Zhou, Du

    2017-03-01

    We report the complete mitochondrial genome (mitogenome) of a spiraling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae). The 16 170 bp long genome consists of 13 protein-coding genes, 20 transfer RNAs, 2 ribosomal RNAs, and a control region. The A. dispersus mitogenome also includes a cytb-like non-coding region and shows several variations relative to the typical insect mitogenome. A phylogenetic tree has been constructed using the 13 protein-coding genes of 12 related species from Hemiptera. Our results would contribute to further study of phylogeny in Aleyrodidae and Hemiptera.

  15. (HEMIPTERA: ALEYRODIDAE SOBRE FRÍJOL

    Directory of Open Access Journals (Sweden)

    Luisa Fernanda SUAREZ GONZALEZ

    2015-01-01

    Full Text Available La biología y ecología de la mosca blanca de los invernaderos, Trialeurodes vaporariorum (Westwood (Hemiptera: Aleyroridae, se encuentra ampliamente documentada. Sin embargo, hasta el momento no hay reportes acerca de la tasa de consumo de alimento por este fitófago, un parámetro necesario para modelos de simulación. Por tal motivo, el objetivo de esta investigación fue la medición indirecta del consumo de alimento por el adulto e instares inmaduros de T. vaporariorum basado en metodologías encontradas en la literatura científica. La estimación de la tasa de consumo de alimento por adultos se realizó bajo condiciones de laboratorio, utilizando como unidad experimental una planta de fríjol de dos semanas de edad. El experimento consistió de dos tratamientos: (T1 plantas libres de cualquier fitófago y (T2 plantas sometidas a infestaciones con T. vaporariorum , cada tratamiento con 20 repeticiones y una réplica en el tiempo de todo el experimento. Se registró el peso fresco diariamente en ambos tratamientos. Para el caso de los inmaduros, la unidad experimental consistió en una hoja cotiledónea de fríjol, infestada con una población determinada de inmaduros de cada instar de mosca blanca teniendo así cinco tratamientos (Instar I, II, III, IV y Control cada uno con 17 repeticiones. Se registró diariamente el peso fresco de las hojas. Se obtuvo un consumo promedio de alimento por el adulto de 0,277 ± 0,0042 mg.dia -1 . Para los dos primeros instares ninfales se estimó una tasa de consumo de 0,052 mg.dia -1 , en el tercer instar de 0,14 mg.dia -1 , y para el cuarto instar de 0,19 mg.dia -1 . Un adulto de mosca blanca puede consumir en un día seis veces su peso y un inmaduro ocho veces, gracias a su rápido metabolismo y la constante excreción de mielecilla.

  16. Tip of the clade on the top of the World--the first fossil Lophopidae (Hemiptera: Fulgoromorpha) from the Palaeocene of Tibet.

    Science.gov (United States)

    Szwedo, Jacek; Stroiński, Adam; Lin, Qibin

    2015-06-01

    Lophopidae is a family of planthoppers (Hemiptera: Fulgoromorpha) present today in tropical and subtropical zones of the Old World. The most recent taxonomic studies and phylogeny of these insects do not include the extinct representatives. Therefore, each new discovery of a fossil lophopid is of high interest, giving new insights to their evolutionary history and enabling to test the proposed relationships. The recent findings of extinct Lophopidae in Europe, in various Palaeogene deposits, put in doubts their proposed evolutionary and biogeographic scenario. The new fossil from the Palaeocene of Northern Tibet is related to one of the Lophopidae clades, Apia(+) group, believed to be the most advanced one, and recently distributed in the recent Sundaland-New Guinea-Queensland area. A new genus and species Gesaris gnapo gen. et sp. n. provide information on early lophopids diversity and relationships and demonstrates the necessity for a revision of the existing hypotheses for the initial diversification and distributional pattern of the Lophopidae.

  17. Preparing sternorrhynchous insects (Insecta: Hemiptera: Sternorrhyncha) for microscope examination: Hoyer’s mounting medium

    Science.gov (United States)

    Proper identification of aphids, scale insects, psyllids, and whitefles (Hemiptera: Sternorrhyncha) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare sternorrhynchous specimens on microscope slides for examination and identi...

  18. Preparing soft-bodied arthropods for arthropods for microscope examination: Armored Scales (Insects: Hemiptera: Diaspididae)

    Science.gov (United States)

    Proper identification of armored scales (Hemiptera: Diaspididae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare armored scales specimens on microscope slides for examination and identification. Steps ranging from collect...

  19. Preparing soft-bodied arthropods for microscope examination: Whiteflies (Insecta: Hemiptera: Alyrodidae)

    Science.gov (United States)

    Proper identification of whiteflies (Hemiptera:Alyrodidae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare whitefly specimens on microscope slides for examination and identification. Steps ranging from collection, specimen...

  20. Preparing soft-bodied arthropods for arthropods for microscope examination: Mealybugs (Insects: Hemiptera: Pseudococcidae)

    Science.gov (United States)

    Proper identification of mealybugs (Hemiptera: Pseudococcidae) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare mealybug specimens on microscope slides for examination and identification. Steps ranging from collection, spec...

  1. Preparing soft-bodied arthropods for microscope examination: Aphids (Insecta: Hemiptera: Aphididae)

    Science.gov (United States)

    Proper identification of aphids (Hemiptera: Aphididae) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare aphid specimens on microscope slides for examination and indentification. Steps ranging from collection, specimen clear...

  2. Variation in male and female genitalia among ten species of North American Anthocoris (Hemiptera: Heteroptera: Anthocoridae)

    Science.gov (United States)

    We compared morphology of internal reproductive anatomy and genitalia among 10 species of North American Anthocoris (Hemiptera: Heteroptera: Anthocoridae). Reproductive structures of males, including internal reproductive organs (testes, seminal vesicles, ejaculatory bulb, phallus), the left parame...

  3. Phylogenetic divergences of the true bugs (Insecta: Hemiptera: Heteroptera), with emphasis on the aquatic lineages

    DEFF Research Database (Denmark)

    Wang, Yan-hui; Cui, Ying; Rédei, Dávid;

    2016-01-01

    Heteroptera are among the most diverse hemimetabolous insects. Seven infraorders have been recognized within this suborder of Hemiptera. Apart from the well-established sister-group relationship between Cimicomorpha and Pentatomomorpha (= Terheteroptera), the two terminal lineages, the relationsh...

  4. Sinopse dos Chryxinae (Hemiptera, Reduviidae Synopsis of the Chryxinae (Hemiptera, Reduviidae

    Directory of Open Access Journals (Sweden)

    Hélcio R. Gil-Santana

    2007-03-01

    Full Text Available Uma sinopse da taxonomia dos Chryxinae com chaves para os gêneros e espécies da subfamília são apresentados. Chryxus bahianus sp. nov. e o macho de Wygodzinskyella travassosi (Lent & Wygodzinsky, 1944 são descritos.Synopsis of the Chryxinae with keys to the genera and species of the subfamily are presented. Chryxus bahianus sp. nov. and the male of Wygodzinskyella travassosi (Lent & Wygodzinsky, 1944 are described.

  5. Pentatomiana beckerae gen. nov. and sp. nov., a new Neotropical Pentatomini (Hemiptera, Heteroptera, Pentatomidae

    Directory of Open Access Journals (Sweden)

    Jocélia Grazia

    2004-06-01

    Full Text Available A new genus, Pentatomiana, and a new species, P. beckerae, are described, based on specimens from Rio de Janeiro and Santa Catarina States, Brazil. Brachyptery is present in the males of the new species. Pentatomiana gen. nov. is compared with Lojus McDonald, 1982, which also presents brachyptery in one of the species, but in females. Illustrations of male and female external genitalia are provided.Um novo gênero, Pentatomiana, e uma nova espécie, P. beckerae, são descritos, com base em espécimes do Rio de Janeiro e de Santa Catarina, Brasil. Machos da nova espécie apresentam braquipteria. Pentatomiana gen. nov. é comparado com Lojus McDonald, 1982, o qual também apresenta braquipteria em uma das espécies, mas em fêmeas. São fornecidas ilustrações da genitália externa masculina e feminina.

  6. Biology and harmfulness of Planococcus vovae (Nassonov (Hemiptera: Pseudococcidae in Belgrade area

    Directory of Open Access Journals (Sweden)

    Draga Graora

    2014-03-01

    Full Text Available Planococcus vovae (Nassonov (Hemiptera: Pseudococcidae is an important pest on plants of the family Cupressaceae. Its numerous populations have been present in recent years on Juniperus spp. in Belgrade. Feeding by sap-sucking on all aboveground plant organs, it causes growth stagnation, chlorosis, drying of needles and branches, and even of entire plants under heavy infestation. Additionally, the scale excretes large quantities of honeydew, on which sooty mold develops, reducing photosynthesis and causing faster plant deterioration. Throughout 2007 and 2008, P. vovae was recorded on Juniperus spp. in 12 localities in Belgrade, and on Thuja sp. in a single locality. The pest was found to develop three generations per year and overwinter on branches at the egg or second instar stages. The first generation adults were observed at the end of May, the second generation at the beginning of August, while the third generation was recorded at the beginning of October. Different overwintering modes, and variable oviposition, embryonic and larval development periods led to an overlapping of generations and continuous presence of all developmental stages on plants. In different localities the infestation of plants varied in abundance from a few individual specimens to very large colonies. The highest infestation intensity was recorded in the localities Bežanija, Dorćol and Voždovac. The predatory species Chrysoperla carnea (Stephens (Neuroptera: Chrysopidae and Nephus bipunctatus (Kugelann (Coleoptera: Coccinelidae were found in the scale colonies. Regarding N. bipunctatus, this was its first record as a new species in the Serbian fauna.

  7. Ecology and management of the woolly whitefly (Hemiptera: Aleyrodidae), a new invasive citrus pest in Ethiopia.

    Science.gov (United States)

    Belay, Difabachew K; Zewdu, Abebe; Foster, John E

    2011-08-01

    Distribution and importance of woolly whitefly (Aleurothrixus floccosus) (Maskell) (Hemiptera: Aleyrodidae), was studied in Ethiopia with an evaluation of treatments against it. Results showed that the pest is distributed in most citrus-growing parts of the country equally infesting all types of citrus crops. Only one pupal parasitoid, Amitus sp., was recorded at Melkaoba. During 2006-2007, eight treatments gave better control of woolly whitefly compared with the control: endod (Phytolacca dodecandra L'Herit) berry extract, white oil 80%, neem oil, omo detergent soap, band application of gasoline, cyhalothrin (karate) 5% EC, selecron (profenofos) 500 EC, and rimon (novaluron) 10 EC. Treatments were applied on 6-8 yr-old orange trees at Melkaoba and Nazareth. At Melkaoba, application of cyhalothrin, selecron, white oil, and Neem gave better control of woolly whitefly compared with the control. All the treatments resulted in a lower number of ants than the control. Ants disrupt biocontrol agents of honeydew-secreting pests, including woolly whiteflies. Mean infestation score was higher in the control than the rest of the treatments. Similarly, at Nazareth, woolly whitefly numbers were lower recorded on cyhalothrin-treated plants. However, the numbers of eggs were significantly higher in endod extract-sprayed plants than the control. All treatments controlled ants better than the control except endod. Infestation scores were lower on endod- and cyhalothrin-treated plants than the control. Mean number of adult woolly whiteflies and eggs were significantly higher on newly grown leaves than older leaves. In general, the number of live adult woolly whiteflies showed a decreasing trend at both sites after treatment applications compared with the control.

  8. Diaspididae (Hemiptera, Coccoidea in sori of two fern species

    Directory of Open Access Journals (Sweden)

    Marcelo Santos

    2015-12-01

    Duas Espécies de Cochonilhas (Hemiptera: Diaspididae Associadas com Soros de Samambaias Resumo. A presente comunicação relata a presença de duas espécies de cochonilhas Hemiberlesia palmae (Cockerell e Pinnaspis strachani (Cooley (Coccoidea, Diaspididae, associadas respectivamente com Asplenium serratum L. (Aspleniaceae e Niphidium crassifolium (L. Lellinger (Polypodiaceae. É o primeiro registro de uma samambaia como planta hospedeira de Hemiberlesia palmae.  Nas duas espécies de samambaias, os diaspidídeos encontravam-se concentrados principalmente ao redor dos soros.

  9. Present status of Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) in the Mariana Islands and its control by two fortuitously introduced natural enemies.

    Science.gov (United States)

    Reddy, G V P; Muniappan, R; Cruz, Z T; Naz, F; Bamba, J P; Tenorio, J

    2009-08-01

    The mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), attacks ornamental and fruit crops in the Mariana Islands. Insecticides cannot penetrate the heavy layers of wax that protect the insect's body. We surveyed the mealybug's locally recruited natural enemies and their effects on its population on Guam, Rota, Saipan, and Tinian to assess the need for introduction of exotic natural enemies. We monitored population densities of M. hirsutus, those of its natural enemies, and parasitism rates for 3 yr, 2005-2007. Our surveys revealed the presence of two parasitoids, Anagyrus kamali Moursi (Hymenoptera: Encyrtidae) and Allotropa sp. near mecrida (Walker) (Hymenoptera: Platygastridae), fortuitously introduced to the Mariana Islands with M. hirsutus. The predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) also was often found feeding on M. hirsutus. Population density of M. hirsutus was below the economic threshold at all locations. Rainfall seemed to affect mean numbers of M. hirsutus and mean numbers of eggs at some locations. On all four islands, the two parasitoids, complemented by the predator, were effectively controlling the M. hirsutus population. No evidence of hyperparasitism was recorded. Currently, economic damage by M. hirsutus is not a concern in the Mariana Islands, and additional parasitoids need not be introduced to control M. hirsutus.

  10. Dysmicoccus de la Región Neotropical (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    María Cristina GRANARA de WILLINK

    2009-01-01

    Full Text Available Se describen e ilustran 16 especies: D. alius sp. nov., D. bonaerensis sp. nov., D. cacao sp. nov., D. caribensis sp. nov., D. fusagasugae sp. nov., D. gracilis sp. nov., D. patagonicus sp. nov., D. perotensis sp. nov., D. pini sp. nov., D. plenus sp. nov., D. rex sp. nov., D. tillandsiae sp. nov., D. tococae sp. nov., D. umbambae sp. nov., D. varius sp. nov., D. vescum sp. nov. y se diagnostican 19: D. boninsis (Kuwana, D. brachydactylus Miller & McKenzie, D. brevipes (Cockerell, D. dactylus Miller & McKenzie, D. dariensis Williams & Granara de Willink, D. grassi (Leonardi, D. hambletoni Wlliams & Granara de Willink, D. hurdi McKenzie, D. inquilinus (Newstead, D. joannesiae (Costa Lima, D. mackenziei Beardsley, D. neobrevipes Beardsley, D. pinicolus McKenzie, D. probrevipes (Morrison, D. radicis Green, D. rapaneae Williams & Granara de Willink, D. sylvarum Williams & Granara de Willink, D. texensis (Tinsley y D. tibouchinae (Hambleton. Se cita por primera vez para la Argentina, D. brachydactylus; para Costa Rica, D. hambletoni; para Cuba, D. hurdi y para Uruguay, D. brevipes. Las nuevas especies fueron encontradas en: Argentina: D. alius, D. bonaerensis y D, patagoniensis; Brasil: D. tococae y D. umbambae; Colombia: D. cacao, D. fusagasugae y D. vescum; Costa Rica: D. rex; Puerto Rico: D. plenum; México: D. perotensis, D, pini y D. tillandsiae; Brasil y Uruguay: D. umbambae; Colombia y Venezuela: D. caribensis y D. varius; Brasil, Costa Rica y Guyana: D. gracilis. Se incluye una clave para las 35 especies de Dysmicoccus de la región neotropical, que afectan 178 especies reunidas en 50 familias vegetales; se amplía la distribución de varias especies.

  11. Three new species of Kerria (Hemiptera: Coccoidea: Tachardiidae) from India.

    Science.gov (United States)

    Ahmad, Ayashaa; Ramamurthy, V V; Sharma, K K; Mohanasundaram, A; Vidyarthi, A S; Ramani, R

    2013-11-07

    Three new species of Kerria Targioni-Tozzetti from India, namely Kerria pennyae Ahmad & Ramamurthy sp. nov. on Schleichera oleosa from Orissa, Kerria dubeyi Ahmad & Ramamurthy sp. nov. on Ficus bengalensis from Bangalore and Kerria varshneyi Ahmad & Ramamurthy sp. nov. on Ziziphus mauritiana from Punjab are described and illustrated, and a key is provided to species of Kerria known from India.

  12. Sp(2) Renormalization

    CERN Document Server

    Lavrov, Peter M

    2010-01-01

    The renormalization of general gauge theories on flat and curved space-time backgrounds is considered within the Sp(2)-covariant quantization method. We assume the existence of a gauge-invariant and diffeomorphism invariant regularization. Using the Sp(2)-covariant formalism one can show that the theory possesses gauge invariant and diffeomorphism invariant renormalizability to all orders in the loop expansion and the extended BRST symmetry after renormalization is preserved. The advantage of the Sp(2)-method compared to the standard Batalin-Vilkovisky approach is that, in reducible theories, the structure of ghosts and ghosts for ghosts and auxiliary fields is described in terms of irreducible representations of the Sp(2) group. This makes the presentation of solutions to the master equations in more simple and systematic way because they are Sp(2)- scalars.

  13. Sp(2) renormalization

    Energy Technology Data Exchange (ETDEWEB)

    Lavrov, Peter M., E-mail: lavrov@tspu.edu.r [Department of Mathematical Analysis, Tomsk State Pedagogical University, Kievskaya St. 60, Tomsk 634061 (Russian Federation)

    2011-08-11

    The renormalization of general gauge theories on flat and curved space-time backgrounds is considered within the Sp(2)-covariant quantization method. We assume the existence of a gauge-invariant and diffeomorphism invariant regularization. Using the Sp(2)-covariant formalism one can show that the theory possesses gauge-invariant and diffeomorphism invariant renormalizability to all orders in the loop expansion and the extended BRST-symmetry after renormalization is preserved. The advantage of the Sp(2) method compared to the standard Batalin-Vilkovisky approach is that, in reducible theories, the structure of ghosts and ghosts for ghosts and auxiliary fields is described in terms of irreducible representations of the Sp(2) group. This makes the presentation of solutions to the master equations in more simple and systematic way because they are Sp(2)-scalars.

  14. Dysmicoccus de la Región Neotropical (Hemiptera: Pseudococcidae Dysmicoccus from the Neotropical Region (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    María Cristina Granara de Willink

    2009-06-01

    Full Text Available Se describen e ilustran 16 especies: D. alius sp. nov., D. bonaerensis sp. nov., D. cacao sp. nov., D. caribensis sp. nov., D. fusagasugae sp. nov., D. gracilis sp. nov., D. patagonicus sp. nov., D. perotensis sp. nov., D. pini sp. nov., D. plenus sp. nov., D. rex sp. nov., D. tillandsiae sp. nov., D. tococae sp. nov., D. umbambae sp. nov., D. varius sp. nov., D. vescum sp. nov. y se diagnostican 19: D. boninsis (Kuwana, D. brachydactylus Miller & McKenzie, D. brevipes (Cockerell, D. dactylus Miller & McKenzie, D. dariensis Williams & Granara de Willink, D. grassi (Leonardi, D. hambletoni Wlliams & Granara de Willink, D. hurdi McKenzie, D. inquilinus (Newstead, D. joannesiae (Costa Lima, D. mackenziei Beardsley, D. neobrevipes Beardsley, D. pinicolus McKenzie, D. probrevipes (Morrison, D. radicis Green, D. rapaneae Williams & Granara de Willink, D. sylvarum Williams & Granara de Willink, D. texensis (Tinsley y D. tibouchinae (Hambleton. Se cita por primera vez para la Argentina, D. brachydactylus; para Costa Rica, D. hambletoni; para Cuba, D. hurdi y para Uruguay, D. brevipes. Las nuevas especies fueron encontradas en: Argentina: D. alius, D. bonaerensis y D, patagoniensis; Brasil: D. tococae y D. umbambae; Colombia: D. cacao, D. fusagasugae y D. vescum; Costa Rica: D. rex; Puerto Rico: D. plenum; México: D. perotensis, D, pini y D. tillandsiae; Brasil y Uruguay: D. umbambae; Colombia y Venezuela: D. caribensis y D. varius; Brasil, Costa Rica y Guyana: D. gracilis. Se incluye una clave para las 35 especies de Dysmicoccus de la región neotropical, que afectan 178 especies reunidas en 50 familias vegetales; se amplía la distribución de varias especies.Sixteen new species are described and illustrated in the present paper: D. alius sp. nov., D. bonaerensis sp. nov., D. cacao sp. nov., D. caribensis sp. nov., D. fusagasugae sp. nov., D. gracilis sp. nov., D. patagonicus sp. nov., D. perotensis sp. nov., D. pini sp. nov., D. plenus sp. nov. D

  15. Novas espécies de Sundarion Kirkaldy (Hemiptera, Membracidae New species of Sundarion Kirkaldy (Hemiptera, Membracidae

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    Antonio José Creão-Duarte

    2005-03-01

    Full Text Available Quatro novas espécies de Sundarion Kirkaldy, 1904 são descritas e ilustradas: S. compactum Souza & Rothéa sp. nov., S. costaricense Souza & Rothéa sp. nov., S. notabile Souza & Rothéa sp. nov. e S. rubricatum Souza & Rothéa sp. nov. As duas primeiras são da Costa Rica e as outras do Brasil, Mato Grosso.Four new species of Sundarion Kirkaldy, 1904 are described and illustrated: S. compactum Souza & Rothéa sp. nov., S. costaricense Souza & Rothéa sp. nov., S. notabile Souza & Rothéa sp. nov., and S. rubricatum Souza & Rothéa sp. nov. The first two species are from Costa Rica, and two others from Brazil, Mato Grosso.

  16. El género Apiococcus Hempel (Hemiptera, Eriococcidae, con redescripción de dos especies The genus Apiococcus Hempel (Hemiptera, Eriococcidae, with redescription of two species

    Directory of Open Access Journals (Sweden)

    Patricia González

    2013-03-01

    Full Text Available The genus Apiococcus Hempel (Hemiptera, Eriococcidae, with redescription of two species. Apiococcus Hempel is a genus from Brazil composed of four gall-inducing species. The adult females of two species, Apiococcus globosus Hempel and A. singularis Hempel, from Brazil, are redescribed and illustrated. Keys to the species of the genus and their galls are given.

  17. Aclerdidae (Hemiptera: Coccoidea de la Argentina Aclerdidae (Hemiptera: Coccoidea from Argentina

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    María Cristina Granara de Willink

    2004-12-01

    Full Text Available Se describen e ilustran la hembra adulta, el primer estadio y un estadio intermedio de la hembra de Aclerda colihuensis sp. nov., encontrada sobre Chusquea culeou una Bambusaceae, de los bosques andinopatagónicos. Se brinda una clave para las especies de Aclerda Signoret, de la región Neotropical. Se cita esta familia por primera vez para la Argentina.The female, the first stage, and intermediate female stages of Aclerda colihuensis n. sp., are described and illustrated. A key to the neotropical species for Aclerda Signoret is given. This is the first family report from Argentina. The new species was found on Chusquea culeou Desv. Bambusaceae, from the Patagonia forest.

  18. Do Scaphoideus titanus (Hemiptera: Cicadellidae) nymphs use vibrational communication?

    Science.gov (United States)

    Chuche, Julien; Thiéry, Denis; Mazzoni, Valerio

    2011-07-01

    Small Auchenorrhyncha use substrate-borne vibrations to communicate. Although this behaviour is well known in adult leafhoppers, so far no studies have been published on nymphs. Here we checked the occurrence of vibrational communication in Scaphoideus titanus (Hemiptera: Cicadellidae) nymphs as a possible explanation of their aggregative distributions on host plants. We studied possible vibratory emissions of isolated and grouped nymphs, as well as their behavioural responses to vibration stimuli that simulated presence of conspecifics, to disturbance noise, white noise and predator spiders. None of our synthetic stimuli or pre-recorded substrate vibrations from nymphs elicited specific vibration responses and only those due to grooming or mechanical contacts of the insect with the leaf were recorded. Thus, S. titanus nymphs showed to not use species-specific vibrations neither for intra- nor interspecific communication and also did not produce alarm vibrations when facing potential predators. We conclude that their aggregative behaviour is independent from a vibrational communication.

  19. Complete mitochondrial genome of Drabescoides nuchalis (Hemiptera: Cicadellidae).

    Science.gov (United States)

    Wu, Yunfei; Dai, Renhuai; Zhan, Hongping; Qu, Ling

    2016-09-01

    The complete mitochondrial genome (mitogenome) of Drabescoides nuchalis (Hemiptera: Cicadellidae) was sequenced. It is 15 309 bp in length with 75.62% (A + T) content and comprises 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNA genes, and a non-coding region (GenBank accession no. KR349344). Gene order is identical to that of the inferred ancestral insect genome. All PCGs start with an ATN codon and terminate with TAA except ND4, which has an incomplete stop codon (T). The anticodons are identical to those of Drosophila yakuba. The phylogenetic tree confirms D. nuchalis and two Cicadellidae species are clustered into a clade, and Cicadellidae is a monophyletic group and provides support for the sister relationship of leafhopper and treehopper.

  20. Effects of irradiation on Planococcus minor (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Ravuiwasa, Kaliova Tavou; Lu, Kuang-Hui; Shen, Tse-Chi; Hwang, Shaw-Yhi

    2009-10-01

    Irradiation has been recognized and endorsed as a potential phytosanitary measure that could be an alternative to current quarantine treatments. Dosages of 50, 100, 150, 200, and 250 Gy were used to irradiate three different life stages (eggs, immatures, and adults) of Planococcus minor (Maskell) (Hemiptera: Pseudococcidae), focusing on females due to its parthenogenesis ability, with an aim to find the most tolerant stage and the most optimal dose to control P. minor. Cobalt 60 was the source of irradiation used. Irradiation of 150-250 Gy has a significant effect on all life stages of P. minor, decreasing its survival rate, percentage of adult reproduction, oviposition, and fertility rate. The adult was the most tolerant life stage in both mortality and fertility rate. All the different irradiated target life stage groups oviposited eggs, but none of the F2 eggs hatched at the most optimal dosage of 150-250 Gy.

  1. Scale Insects (Hemiptera: Coccoidea) on Myrciaria dubia (Myrtaceae) in Brazil.

    Science.gov (United States)

    Wolff, V R S; Kondo, T; Peronti, A L B G; Noronha, A C S

    2016-06-01

    Commercial cultivation of the fruit tree Myrciaria dubia (Myrtaceae) is being developed in Brazil but phytophagous insects, including scale insects (Hemiptera: Coccoidea), can become pests in plantations. The coccids Ceroplastes jamaicensis White, Coccus viridis (Green), Parasaissetia nigra (Nietner), Pseudokermes vitreus (Cockerell) (Coccidae), and the diaspidid Pseudaonidia trilobitiformis (Green) were collected on M. dubia in the municipality of Belém and Tomé-Açu, state of Pará (PA), metropolitan and Northeast Pará mesoregions, Brazil. A key to species of Coccoidea recorded on M. dubia, based on adult females, is provided. Photographs for all scale insects reported on M. dubia are provided. Ceroplastes jamaicensis is recorded for the first time for Brazil and is herein reported for the first time associated with this host.

  2. The complete mitochondrial genome of Bemisia afer (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Wang, Hua-Ling; Xiao, Na; Yang, Jiao; Wang, Xiao-Wei; Colvin, John; Liu, Shu-Sheng

    2016-01-01

    The length of the Bemisia afer (Priesner & Hosny) (Hemiptera: Aleyrodidae) mitochondrial genome (mitogenome) is 14,968 bp and consists of 13 protein coding genes (PCGs), 21 transfer RNAs (tRNA), 2 ribosomal RNAs and 1 control region. Apart from one serine transfer RNA gene (tRNA-Ser) which is absent, the synteny is consistent with the mitogenomes of other whitefly species. The overall base composition of the heavy strand for A, G, T and C is 28.96, 18.97, 36.7 and 15.37%, respectively, with a slight AT bias. Two rare codons (GTG and TTG) are employed as start codons by some PCGs. B. afer is a group of cryptic species. This first mitogenome cloned from African cassava B. afer, therefore, both enrich the whitefly molecular resource and will aid the sequencing of the other species' mitogenomes. It will contribute significantly to resolving the systematics of the B. afer complex.

  3. Quatro espécies novas de Tropicanus DeLong (Hemiptera, Cicadellidae Four new species of Tropicanus DeLong (Hemiptera: Cicadellidae

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    Keti M. R. Zanol

    2006-12-01

    Full Text Available São descritas quatro novas espécies no subgênero Tropicanus: T. taulipangus sp. nov., T. amazonicus sp. nov., T. chapadensis sp. nov. e T. lisei sp. nov., todas para o Brasil.Four new species of Tropicanus are described in the subgenus Tropicanus: T. taulipangus sp. nov., T. amazonicus sp. nov., T. chapadensis sp. nov. and T. lisei sp. nov., all from Brazil.

  4. Effect of insect density and host plant quality on wing-form in Megamelus scutellaris (Hemiptera: Delphacidae)

    Science.gov (United States)

    Megamelus scutellaris Berg (Hemiptera: Delphacidae) is a South American species that feeds on waterhyacinth, Eichhornia crassipes Mart. (Solms). This species exhibits significant wing dimorphism whereby fully winged adults (macropters) are capable of flight while those with reduced wings (brachtypt...

  5. Letalidad de hongos entomopatogenos sobre Dysdercus peruvianus (Hemiptera: Pirrhocoridae

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    Francesca Falconi

    2011-05-01

    Full Text Available Se evaluó la patogenicidad y virulencia sobre Dysdercus peruvianus de una cepa de Acremonium y una de Scopulariopsis, aisladas de adultos infectados de D. peruvianus procedentes de Mala, Provincia de Cañete. También se evaluó una cepa de Beauveria sp. aislada de Schistocerca piceifrons peruviana procedente de Ayacucho. Los bioensayos se realizaron sobre ninfas del cuarto estadío de D. peruvianus y las concentraciones empleadas fueron 3,7x108, 1,9x108, 9,4x107 conidias/mL para Beauveria sp., Acremonium sp. y Scopulariopsis sp. respectivamente. Veinte días posteriores al tratamiento, los mayores porcentajes de mortalidad los causaron Beauveria sp. (83,3% y Acremonium sp. (80%. Scopulariopsis sp. causo una mortalidad de 23,3%. Acremonium sp. fue la cepa más agresiva con un tiempo de letalidad (TL50 3,8 días.

  6. Inventory and assessment of foliar natural enemies of the soybean aphid (Hemiptera: Aphididae) in South Dakota

    Science.gov (United States)

    Soybean aphid (Aphis glycines Matsumura) (Hemiptera: Aphididae) is a major pest of soybean in northern production regions of North America, and insecticides have been the primary management approach while alternative methods are developed. Knowledge of arthropod natural enemies and their impact on ...

  7. Preparing soft-bodied arthropods for microscope examination: Soft Scales (Insecta: Hemiptera: Coccidae)

    Science.gov (United States)

    Proper identification of soft scales (Hemiptera:Coccidae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare soft scale specimens on microscope slides for examination and identification. Steps ranging from collection, speci...

  8. Micro-CT study of the anatomy of the Leafhopper Homalodisca vitripennis (Hemiptera: Cicadellidae)

    Science.gov (United States)

    A Digital Anatomy Library, DAL, was produced to the anatomy of the glassy-winged sharpshooter adult, Homalodisca vitripennis (Hemiptera: Cicadellidae), vector of bacteria which cause Pierce’s disease of grapevines. The insect anatomy was elucidated using a high resolution Bruker Skyscan 1172 micro t...

  9. Contributions to the knowledge of Banasa Stål (Hemiptera, Heteroptera, Pentatomidae: Banasa chaca Thomas

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    Thereza de Almeida Garbelotto

    2011-09-01

    Full Text Available Contributions to the knowledge of Banasa Stål (Hemiptera, Heteroptera, Pentatomidae: Banasa chaca Thomas. The male of Banasa chaca Thomas is described with emphasis on external and internal genitalia and the female internal genitalia is described. Banasa chaca is newly recorded from Buenos Aires Province (Argentina.

  10. A redescription of the endemic Madagascan genus Tricompastes (Hemiptera: Heteroptera: Pentatomidae).

    Science.gov (United States)

    Kment, Petr; Baena, Manuel

    2015-11-17

    The endemic Madagascan genus Tricompastes Cachan, 1952 (Hemiptera: Heteroptera: Pentatomidae: Pentatominae: Triplatygini), containing a single species-Tricompastes gigas Cachan, 1952, is redescribed and illustrated, including first descriptions of male and female genitalia. First exact localities of the species are provided. Lectotype of T. gigas is designated.

  11. Effects of founder population size on the performance of Orius laevigatus (Hemiptera: Anthocoridae) colonies

    NARCIS (Netherlands)

    Castañe, C.; Bueno, V.H.P.; Carvalho, L.M.; Lenteren, van J.C.

    2014-01-01

    Orius laevigatus (Hemiptera: Anthocoridae) is a key predator of thrips and is mass reared in large numbers for use in biological control. The aim of this study was to evaluate the effect of founder population size on the biological and behavioral performance of O. laevigatus over time. Laboratory li

  12. Use of pheromones for monitoring phytophagous stink bug (Hemiptera: Pentatomidae) populations

    Science.gov (United States)

    Phytophagous native stink bugs (Hemiptera: Pentatomidae), including Euschistus spp., Nezara viridula (L.), Chinavia hilaris (Say), Plautia stali Scott, Chlorochroa spp., and Thyanta spp., are primary pests responsible for millions of dollars in losses and cost of control in most fruit, vegetable, gr...

  13. Field evaluation of potential pheromone lures for Lygus lineolaris (Hemiptera: Miridae) in the Mid-South

    Science.gov (United States)

    Plant bugs (Hemiptera: Miridae) are phytophagous pests of cultivated plants around the world. In the mid-South region of the United States, Lygus lineolaris is a primary pest of cotton, and causes economic damage. Previously published research about the volatiles produced by members of the genus Lyg...

  14. Attraction of stink bug (Hemiptera: Pentatomidae) nymphs to Euschistus aggregation pheromone in the field

    Science.gov (United States)

    Phytophagous stink bugs (Hemiptera: Pentatomidae) are primary pests in most fruit, vegetable, grain, and row crops worldwide. Pheromones have been identified and synthesized for several species of economically important stink bug pests. When yellow pyramid traps are baited with lures containing thes...

  15. Phylogenetics and evolutionary morphology of the Neotropical true bug genus Epipolops (Hemiptera: Heteroptera: Geocoridae)

    Science.gov (United States)

    Species of Epipolops Herrich-Schaeffer (Hemiptera: Geocoridae), comprising the largest genus of Pamphantinae, are among the most bizarre true bugs because of their striking morphology. To elucidate evolutionary morphology in Epipolops, a phylogenetic analysis was performed using 17 species and 36 ad...

  16. Gut content analysis of a phloem-feeding insect, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae)

    Science.gov (United States)

    Potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) is a key pest of potato (Solanum tuberosum L., Solanales: Solanaceae) and a vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. In addition to its presence on cultivated crops, the p...

  17. First record of Eggplant Mealybug, Coccidohystrix insolita (Hemiptera: Pseudococcidae, on Guam: Potentially a major pest

    Directory of Open Access Journals (Sweden)

    Aubrey Moore

    2014-01-01

    Full Text Available The eggplant mealybug, Coccidohystrix insolita (Green (Hemiptera: Pseudococcidae, is recorded from the island of Guam in the Mariana Islands for the first time. Factors indicating that this introduced mealybug has the potential to become a pest of economic importance for agriculture and horticulture on Guam are discussed.

  18. DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization...

  19. Genes expressed in field-caught pink hibiscus mealybugs, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    We advanced the understanding of the biology of an invasive pest, the pink hibiscus mealybug, PHM, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) by using a genomics approach to identify genes expressed within field collected PHM. The information produced provides valuable, new and unique info...

  20. First record of Eggplant Mealybug, Coccidohystrixinsolita (Hemiptera: Pseudococcidae), on Guam: Potentially a major pest.

    Science.gov (United States)

    Moore, Aubrey; Watson, Gillian W; Bamba, Jesse

    2014-01-01

    The eggplant mealybug, Coccidohystrixinsolita (Green) (Hemiptera: Pseudococcidae), is recorded from the island of Guam in the Mariana Islands for the first time. Factors indicating that this introduced mealybug has the potential to become a pest of economic importance for agriculture and horticulture on Guam are discussed.

  1. Proteins expressed in the pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    We produced a dataset of 315 protein sequences which we isolated from the pink hibiscus mealybug, PHM, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae). The dataset was published under accession numbers: EF070444-EF070605 and EF092085-EF091933, in the National Center for Biotechnology Informatio...

  2. Wolbachia infection differs among divergent mitochondrial haplotypes of Bactericera cockerelli (Hemiptera: Triozidae)

    Science.gov (United States)

    Four mitochondrial (cytrochrome oxidase I) haplotypes of the potato psyllid, Bactericera cockerelli (S'ulc) (Hemiptera: Triozidae), have been identified in North America: western, central, northwestern,and southwestern. A recent study found that females of the northwestern haplotype mated by males o...

  3. Phylogeographic analysis of Harrisia cactus mealybug, Hypogeococcus pungens (Hemiptera: Pseudoccidae) populations: work in progress

    Science.gov (United States)

    Harrisia cactus mealybug (HCM), Hypogeococcus pungens (Hemiptera: Pseudoccidae) Granara de Willink (1981) is infesting and killing cacti in the southern coast of Puerto Rico, covering an area of about 1,400 km2. The 13 species of cacti occurring in Puerto Rico are threatened by this new pest; three...

  4. Resistance for watermelon (Citrullus lanatus var. lanatus) against whiteflies (Hemiptera: Aleyrodidae)

    Science.gov (United States)

    The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is an important global pest with and an extensive host range. Watermelon (Citrullus lanatus var. lanatus) is among the crops damaged by this pest. Host plant resistance is the foundation for the management of crops pests in general. ...

  5. Preliminary Observations on Zelus obscuridorsis (Stål) (Hemiptera: Reduviidae) as Predator of the Corn Leafhopper (Hemiptera: Cicadellidae) in Argentina.

    Science.gov (United States)

    Virla, Eduardo G; Melo, Cecilia M; Speranza, Stefano

    2015-06-03

    The corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae), is an important corn pest in most of tropical and subtropical America. This leafhopper has a rich natural enemy complex of which parasitoids and pathogens are the most studied; knowledge on its predators is limited. We noted the presence of the native assassin bug Zelus obscuridorsis (Hemiptera: Reduviidae) predating diverse motile insects, including the corn leafhopper, on corn plants cultivated in household vegetable gardens in San Miguel de Tucumán (Argentina); in order to verify its predatory actions, we exposed lab-bred individuals of D. maidis to adults of Z. obscuridorsis. The predators were starved for 24 h before trials in which the corn leafhopper in different developmental stages were exposed. Zelus obscuridorsis is highly skilled in catching specimens in motion, but it was not able to prey on eggs. The predator was capable to catch and prey on nymphs and adults.

  6. Preliminary Observations on Zelus obscuridorsis (Stål (Hemiptera: Reduviidae as Predator of the Corn Leafhopper (Hemiptera: Cicadellidae in Argentina

    Directory of Open Access Journals (Sweden)

    Eduardo G. Virla

    2015-06-01

    Full Text Available The corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae, is an important corn pest in most of tropical and subtropical America. This leafhopper has a rich natural enemy complex of which parasitoids and pathogens are the most studied; knowledge on its predators is limited. We noted the presence of the native assassin bug Zelus obscuridorsis (Hemiptera: Reduviidae predating diverse motile insects, including the corn leafhopper, on corn plants cultivated in household vegetable gardens in San Miguel de Tucumán (Argentina; in order to verify its predatory actions, we exposed lab-bred individuals of D. maidis to adults of Z. obscuridorsis. The predators were starved for 24 h before trials in which the corn leafhopper in different developmental stages were exposed. Zelus obscuridorsis is highly skilled in catching specimens in motion, but it was not able to prey on eggs. The predator was capable to catch and prey on nymphs and adults.

  7. Main: SP8BFIBSP8AIB [PLACE

    Lifescience Database Archive (English)

    Full Text Available SP8BFIBSP8AIB S000183 16-Feb-2001 (last modified) seki One of SPBF binding site (SP...o (I.b.); SP8BF recognizes both SP8a and SP8b sequences; See also SP8BFIBSP8BIB (S000184); SP8BF activity is

  8. Main: SP8BFIBSP8BIB [PLACE

    Lifescience Database Archive (English)

    Full Text Available SP8BFIBSP8BIB S000184 16-Feb-2001 (last modified) seki One of SPBF binding site (SP...; SP8BF recognizes both SP8a and SP8b sequences; See also SP8BFIBSP8AIB (S000183); SP8BF activity is also fo

  9. A new gall-inducing genus and species of Eriococcidae (Hemiptera: Sternorrhyncha: Coccoidea) on Sapindaceae from Brazil.

    Science.gov (United States)

    Hodgson, Chris; Isaias, Rosy M S; Oliveira, D C

    2013-11-06

    A new gall-inducing genus and species of felt scales (Hemiptera: Coccoidea: Eriococcidae) found on the leaves and twigs of Matayba guianensis (Sapindaceae) in Brazil is described: Bystracoccus Hodgson gen n. and B. mataybae Hodgson, Isaias & Oliveira sp. n.  This is the first record of an eriococcid inducing leaf and stem galls on Sapindaceae and is only the second example of a member of the Eriococcidae to induce stem galls in which the insects diapause during the dry (winter) season.  Only the adult female, second-instar female and crawler are known.  The species overwinters as the first-instar nymph in pit galls on the twigs but spends the rest of the year associated with two-chambered galls on the leaves.  It has recently become clear that South America has a rich felt-scale insect fauna many of which induce galls. It has proved very difficult to place this new genus in a family as it appears to fall between the Eriococcidae and Beesoniidae but is here placed in the eriococcids based on the similarity of the first-instar nymphs and the abundance of this family in the Neotropics.  However, the dorsum of the abdomen of the mature adult female becomes heavily sclerotised, forming a round plug-like structure that completely fills the gall orifice.  This structure shows remarkable morphological similarities to that of the beesoniid Danumococcus parashoreae Takagi & Hodgson found on Parashorea tomentella (Dipterocarpaceae) in Sabah, Malaysia, with which it is compared along with other eriococcid genera known from South America.

  10. Exploration for facultative endosymbionts of glassy-wingedsharpshooter (Hemiptera: Cicadellidae)

    Energy Technology Data Exchange (ETDEWEB)

    Montllor-Curley, C.; Brodie, E.L.; Lechner, M.G.; Purcell, A.H.

    2006-07-01

    Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae),glassy-winged sharpshooter, was collected in California and severalstates in the southeastern United States in 2002 and 2003 and analyzedfor endosymbiotic bacteria. Hemolymph, eggs, and bacteriomes wereexamined for the presence of bacteria by polymerase chain reaction. Asubset of hemolymph and egg samples had their 16S rRNA gene ampliconscloned and sequenced or analyzed by restriction digest patterns ofsamples compared with known bacterial DNA. Baumannia cicadellinicola, oneof the primary symbionts of glassy-winged sharpshooter, was found in themajority of hemolymph samples, although it has been considered until nowto reside primarily inside the specialized host bacteriocytes. Wolbachiasp., a common secondary symbiont in many insect taxa investigated todate, was the second most frequently detected bacterium in hemolymphsamples. In addition, we detected bacteria that were most closely related(by 16S rRNA gene sequence) to Pseudomonas, Stenotrophomonas, andAcinetobacter in hemolymph samples of one and/or two glassy-wingedsharpshooters, but their origin is uncertain.

  11. How will Mahanarva spectabilis (Hemiptera: Cercopidae) Respond to Global Warming?

    Science.gov (United States)

    Fonseca, M G; Auad, A M; Resende, T T; Hott, M C; Borges, C A V

    2016-01-01

    The aim of this study was to determine the favorable constant temperature range for Mahanarva spectabilis(Distant) (Hemiptera: Cercopidae) development as well as to generate geographic distribution maps of this insect pest for future climate scenarios. M. spectabilis eggs were reared on two host plants (Brachiaria ruziziensis(Germain and Edvard) and Pennisetum purpureum(Schumach)), with individual plants kept at temperatures of 16, 20, 24, 28, and 32 °C. Nymphal stage duration, nymphal survival, adult longevity, and egg production were recorded for each temperature*host plant combination. Using the favorable temperature ranges for M. spectabilis development, it was possible to generate geographic distribution. Nymphal survival was highest at 24.4 °C, with estimates of 44 and 8% on Pennisetum and Brachiaria, respectively. Nymphal stage duration was greater on Brachiaria than on Pennisetum at 20 and 24 °C but equal at 28 °C. Egg production was higher on Pennisetum at 24 and 28 °C than at 20 °C, and adult longevity on Pennisetum was higher at 28 °C than at 20 °C, whereas adult longevity at 24 °C did not differ from that at 20 and 28 °C. With these results, it was possible to predict a reduction in M. spectabilis densities in most regions of Brazil in future climate scenarios.

  12. Application of RNA interference in triatomine(Hemiptera:Reduviidae)studies

    Institute of Scientific and Technical Information of China (English)

    Rafaela M.M.Paim; Ricardo N.Araujo; Michael J.Lehane; Nelder F.Gontijo; Marcos H.Pereira

    2013-01-01

    Triatomines(Hemiptera: Reduviidae)are obligate hematophagous insects.They are of medical importance because they are vectors of Trypanosoma cruzi,the causative agent of Chagas disease in the Americas.In recent years,the RNA interference (RNAi)technology has emerged as a practical and useful alternative means of studying gene function in insects,including triatomine bugs.RNAi research in triatomines is still in its early stages,several issues still need to be elucidated,including the description of the molecules involved in the RNAi machinery and aspects related to phenotype evaluation and persistence of the knockdown in different tissues and organs.This review considers recent applications of RNAi to triatomine research,describing the major methods that have been applied during the knockdown process such as the double-stranded RNA delivery mechanism(injection,microinjection,or ingestion)and the phenotype characterization (mRNA and target protein levels)in studies conducted with the intent to provide greater insights into the biology of these insects.In addition to the characterization of insect biomolecules,some with biopharmacological potential,RNAi may provide a new view of the interaction between triatomine and trypanosomatids,enabling the development of new measures for vector control and transmission of the parasite.

  13. Weed hosts of cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Vennila, S; Prasad, Y G; Prabhakar, M; Agarwal, Meenu; Sreedevi, G; Bambawale, O M

    2013-03-01

    The exotic cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) invaded India during 2006, and caused widespread infestation across all nine cotton growing states. P. solenopsis also infested weeds that aided its faster spread and increased severity across cotton fields. Two year survey carried out to document host plants of P. solenopsis between 2008 and 2010 revealed 27, 83, 59 and 108 weeds belonging to 8, 18, 10 and 32 families serving as alternate hosts at North, Central, South and All India cotton growing zones, respectively. Plant species of four families viz., Asteraceae, Amaranthaceae, Malvaceae and Lamiaceae constituted almost 50% of the weed hosts. While 39 weed species supported P. solenopsis multiplication during the cotton season, 37 were hosts during off season. Higher number of weeds as off season hosts (17) outnumbering cotton season (13) at Central over other zones indicated the strong carryover of the pest aided by weeds between two cotton seasons. Six, two and seven weed hosts had the extreme severity of Grade 4 during cotton, off and cotton + off seasons, respectively. Higher number of weed hosts of P. solenopsis were located at roadside: South (12) > Central (8) > North (3) zones. Commonality of weed hosts was higher between C+S zones, while no weed host was common between N+S zones. Paper furnishes the wide range of weed hosts of P. solenopsis, discusses their significance, and formulated general and specific cultural management strategies for nationwide implementation to prevent its outbreaks.

  14. The scale insects (Hemiptera: Coccoidea) of the Maltese Archipelago.

    Science.gov (United States)

    Mifsud, David; Mazzeo, Gaetana; Russo, Agatino; Watson, Gillian W

    2014-09-25

    Past works on scale insects (Hemiptera: Coccoidea) from the Maltese Archipelago are reviewed. Based on the literature and contemporary collections, a total of 93 species of scale insects belonging to 12 scale insect families are here reported (Aclerdidae 1 species; Asterolecaniidae 4; Coccidae 17; Diaspididae 46; Eriococcidae 5; Kermesidae 1; Margarodidae 1; Micrococcidae 1; Monophlebidae 2; Pseudoccocidae 11; Putoidae 2 and Rhizoecidae 2). Of these, 17 species represent new distribution records. Ten species are excluded from the scale insect fauna of the Maltese Islands. Of the 93 species present, only 29 (31.18%) are probably indigenous and the rest (68.82%) represent established introductions from elsewhere. More than 65% of the indigenous species are typical Mediterranean in distribution, with a few species having a mainly European chorotype. A quarter of the established aliens originate from Eurasia, followed by an East Asian/ Oriental component (20.31%); European (14.06%); Neotropical (14.06%); cryptogenic (14.06%); African (7.81%) and Australasian (4.70%). Movement of live fruit trees and ornamental plants into the Maltese Archipelago from nearby countries is probably the main route for entry of alien scale insects into the country. Some possible future introductions are discussed.

  15. Systematics of Mepraia (Hemiptera-Reduviidae): cytogenetic and molecular variation.

    Science.gov (United States)

    Calleros, L; Panzera, F; Bargues, M D; Monteiro, F A; Klisiowicz, D R; Zuriaga, M A; Mas-Coma, S; Pérez, R

    2010-03-01

    The haematophagous insects of the subfamily Triatominae (Hemiptera-Reduviidae) have great epidemiological importance as vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Mepraia was originally described as a monotypic genus comprised of Mepraia spinolai, distributed along coastal areas of northern Chile (from Region I to the Metropolitan Region). Recently, some M. spinolai populations have been ranked as a new species named Mepraia gajardoi. Several populations along the distribution range of the genus were sampled, and genetic differentiation was studied based upon the analysis of three molecular markers: cytogenetics (karyotype and chromosome behaviour during meiosis using the C-banding technique), mitochondrial DNA (a cytochrome oxidase I gene fragment), and nuclear ribosomal DNA (intergenic region including the two internal transcribed spacers ITS-1 and ITS-2 and the 5.8S rRNA gene). The data here presented indicate that populations within the Mepraia genus (excluding Region II specimens) can be divided into two separate lineages. One lineage is comprised of specimens from the northernmost Region I and represents M. gajardoi. The other includes samples from the southern III, IV and the Metropolitan Regions, and represents M. spinolai. Region II individuals deserve particular attention as their relationship to the two identified lineages is not clear-cut. While they appear to belong to M. spinolai based on cytogenetics and rDNA markers, COI results indicate a closer relationship to M. gajardoi. This disagreement can be due to mitochondrial DNA introgression or the retention of ancestral polymorphisms.

  16. Cytogenetics and genome evolution in the subfamily Triatominae (Hemiptera, Reduviidae).

    Science.gov (United States)

    Panzera, F; Pérez, R; Panzera, Y; Ferrandis, I; Ferreiro, M J; Calleros, L

    2010-01-01

    The subfamily Triatominae (Hemiptera, Reduviidae), vectors of Chagas disease, includes over 140 species. Karyotypic information is currently available for 80 of these species. This paper summarizes the chromosomal variability of the subfamily and how it may reveal aspects of genome evolution in this group. The Triatominae present a highly conserved chromosome number. All species, except 3, present 20 autosomes. The differences in chromosome number are mainly caused by variation in the number of sex chromosomes, due to the existence of 3 sex systems in males (XY, X(1)X(2)Y and X(1)X(2)X(3)Y). However, inter- and intraspecific differences in the position, quantity and meiotic behavior of constitutive heterochromatin, in the total genome size, and in the location of ribosomal 45S rRNA clusters, have revealed considerable cytogenetic variability within the subfamily. This cytogenetic diversity offers the opportunity to perform cytotaxonomic and phylogenetic studies, as well as structural, evolutionary, and functional analyses of the genome. The imminent availability of the complete genome of Rhodnius prolixus also opens new perspectives for understanding the evolution and genome expression of triatomines. The application of fluorescence in situ hybridization for the mapping of genes and sequences, as well as comparative analyses of genome homology by comparative genomic hybridization will be useful tools for understanding the genomic changes in relation to evolutionary processes such as speciation and adaptation to different environments.

  17. A New Genus of Macropsinae (Hemiptera: Cicadellidae) From Madagascar.

    Science.gov (United States)

    Yang, Liyuan; Dietrich, C H; Zhang, Yalin

    2016-01-01

    Paragalboa acuta GEN & SP N: is described and illustrated from Madagascar. The new genus shows morphological affinities to the Macropsini genus Galboa Distant recorded from Seychelles. A checklist of all known genera of Macropsinae is provided.

  18. A review of the tribes of Deltocephalinae (Hemiptera: Auchenorrhyncha: Cicadellidae

    Directory of Open Access Journals (Sweden)

    Chris Dietrich

    2013-05-01

    Full Text Available The classification of the largest subfamily of leafhoppers, Deltocephalinae, including 38 tribes, 923 genera, and 6683 valid species, is reviewed and revised. An updated phylogeny of the subfamily based on molecular (28S, Histone H3 and morphological data and an expanded taxon sample (37 taxa not included in previous analyses is presented. Based on the results of these analyses and on the morphological examination of many representatives of the subfamily, the classification of the tribes and subtribes of Deltocephalinae is revised. Complete morphological descriptions, illustrations, lists of the included genera, and notes on their distribution, ecology, and important vector species are provided for the 38 recognized tribes and 18 subtribes. A dichotomous key to the tribes is provided. All names in the taxonomic treatments are hyperlinked to online resources for individual taxa which are supported by a comprehensive database for Deltocephalinae compiled using the taxonomic database software package 3I. The online functionality includes an interactive key to tribes and subtribes and advanced database searching options. Each taxon (subspecies through subfamily has a unique taxon webpage providing nomenclatural information, lists of included taxa, an automated description (if available, images (if available, distributional information, bibliographic references and links to outside resources. Some observations and trends regarding the history of taxonomic descriptions in Deltocephalinae are reported. Four new tribes are described: Bahitini tribe nov. (25 genera, Bonsapeiini tribe nov. (21 genera, Phlepsiini tribe nov. (4 genera, and Vartini tribe nov. (7 genera. The circumscription and morphological characterization of Scaphoideini Oman, 1943 (61 genera is substantially revised. Eleven new species are described: Acostemma stilleri sp. nov., Arrugada linnavuorii sp. nov., Drabescus zhangi sp. nov., Parabolopona webbi sp. nov., Goniagnathus emeljanovi

  19. [Diversity of Hemiptera Auchenorrhyncha in citrus, coffee and a fragment of native forest of the state of São Paulo].

    Science.gov (United States)

    Giustolin, Teresinha A; Lopes, João R S; Querino, Ranyse B; Cavichioli, Rodney R; Zanol, Kety; Azevedo Filho, Wilson S; Mendes, Miguel A

    2009-01-01

    The population of Hemiptera Auchenorrhyncha was studied in sweet citrus groves (Citrus sinensis), coffee plantations (Coffea arabica) and a semi-deciduous seasonal forest with shrub physiognomy in Bebedouro, SP, to evaluate the influence of the natural ecosystem on the species composition of the agroecosystems. Monitoring was carried out by using yellow stick cards, which were replaced every 15 days and all Auchenorrhyncha collected were counted and identified. Seven families, 11 subfamilies and 98 species were collected, with Cicadellidae being the most abundant. The native forest presented larger wealth, diversity and equitability of Auchenorrhyncha species, demonstrating to be more stable than the other habitats. The high values of similarities obtained between the agroecosystems and the forest demonstrated that great part of Auchenorrhyncha species occurring in the agricultural habitats was also occurring at the forest, indicating that the last may serve as reservoir of species. The abundance of the taxonomic groups of Auchenorrhyncha collected varied with the evaluated habitats, with Proconiini being the most abundant in the coffee plantation next to the forest, Athysanini, Scaphytopiini, Neocoelidiinae and Coelidiinae in the orange orchard and coffee plantation distant from the forest; Cicadellinae and Agalliinae were not related to any of the habitats. The presence of vector insects and possible vectors of plant diseases in the appraised habitats indicate the need of the implementation of strategies for landscape management.

  20. Dinâmica Populacional de Mosca-Branca Bemisia tabaci (Genn. Biótipo B (Hemiptera: Aleyrodidae em Feijoeiro

    Directory of Open Access Journals (Sweden)

    Anderson Silva

    2014-04-01

    Abstract. This study aimed to evaluate the influence of abiotic factors on the population dynamics of the whitefly Bemisia tabaci (Genn., (Hemiptera: Aleyrodidae in three sowing seasons on bean plants (Phaseolus vulgaris L.. The assays were set up from June 2010 to April 2012 and conducted in the Departamento de Fitossanidade at FCAV/UNESP – Campus of Jaboticabal, SP. The studies of the population dynamics of B. tabaci biotype B were performed weekly through infestation assessments of eggs, nymphs and adults of the whitefly on 18 samplings, six for each sowing season: “winter”, “water” and “dry”, in addition to the influence of the abiotic factors weekly temperature and relative humidity (minimum, average and maximum and accumulated rainfall. These variables were correlated to the total number of eggs, nymphs and adults of the whitefly through Pearson’s linear correlation analysis. Among the obtained results, the population peaks of B. tabaci biotype B occurred at the “water” season, followed by “winter” and “dry” seasons, and the abiotic factors temperature and relative humidity influenced negatively the population dynamics of the whitefly.

  1. New records of stink bugs (Hemiptera: Pentatomidae) from Colombia.

    Science.gov (United States)

    Castro-Huertas, Valentina; Schwertner, Cristiano F; Fernández, Fernando

    2015-06-18

    New records of genera and species of stink bugs (Hemiptera: Pentatomidae) from Colombia are provided. Two genera are new records for South America: Alathetus and Schraderiellus. Fifteen genera are new record for Colombia: Agaclitus, Boea, Ceratozygum, Euthyrhynchus, Eritrachys, Doesburguedessa, Lopadusa, Marmessulus, Paralincus, Patanius, Peromatus, Phalaecus, Phoeacia, Rio, and Tyrannocoris. Forty-nine species from five subfamiles are recorded for the first time in Colombia. Asopinae: Coryzorhaphis carneolus Erichson, Coryzorhaphis superba Breddin, Euthyrhynchus floridanus (Linnaeus), Podisus sagitta Fabricius, Stiretrus anchorago (Fabricius), Stiretrus cinctellus Germar, Tylospilus peruvianus Horvath, Tyrannocoris nigriceps Thomas. Cyrtocorinae: Ceratozygum horridum (Germar). Discocephalinae: Agaclitus dromedarius Stål, Antiteuchus melanoleucus (Westwood), Antiteuchus sepulcralis (Fabricius), Dinocoris gibbosus (Fallou), Dinocoris variolosus (Linnaeus), Discocephalessa terminalis (Walker), Dryptocephala crenata Ruckes, Dryptocephala dentifrons (Latreille), Eurystethus ovalis Ruckes, Paralcippus dimidiatus (Ruckes), Alathetus rufitarsus Dallas, Eritrachys bituberculata Ruckes, Paralincus bimaculatus (Ruckes), Schraderiellus cinctus (Ruckes), Xynocoris recavus (Garbelotto & Campos). Edessinae: Brachystethus cribus (Fabricius), Brachystethus tricolor Bolívar, Doesburguedessa elongatispina Fernandes and Lopadusa fuscopunctata (Distant). Pentatominae: Banasa fulgida Thomas, Banasa paraexpallescens Thomas, Dichelops divisus (Walker), Dichelops nigrum Bergroth, Euschistus carbonerus Rolston, Mormidea bovilla (Distant), Mormidea triangularis (Walker), Murgantia bifasciata Herrich-Schaeffer, Murgantia violascens (Westwood), Oebalus pugnax (Fabricius), Oebalus ypsilon-griseus (DeGeer), Odmalea concolor (Walker), Patanius vittatus Rolston, Proxys albopunctulatus (Palisot), Proxys punctulatus (Palisot), Rhyncholepta grandicallosa Bergroth, Rio insularis Ruckes, Roferta

  2. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    Directory of Open Access Journals (Sweden)

    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  3. Primer Registro para el Perú de Brachycaudus schwartzi (Bórner (Hemiptera: Aphididae

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    Walter Díaz

    2013-04-01

    Full Text Available Se registra por primera vez para el Perú la especie Brachycaudus schwartzi (Bórner (Hemiptera: Aphididae, “pulgón pardo del duraznero”. La especie ha sido colectada en el departamento de Tacna. Material de referencia se encuentra depositado en la Colección Referencial de Insectos de la Unidad del Centro de Diagnóstico de Sanidad Vegetal del SENASA, Lima, Perú.

  4. Seasonal Effects on the Population, Morphology and Reproductive Behavior of Narnia femorata (Hemiptera: Coreidae)

    OpenAIRE

    Lauren A. Cirino; Christine W Miller

    2017-01-01

    Many insects are influenced by the phenology of their host plants. In North Central Florida, Narnia femorata (Hemiptera: Coreidae) spends its entire life cycle living and feeding on Opuntia mesacantha ssp. lata. This cactus begins producing flower buds in April that lead to unripe green fruit in June that ripen into red fruit through December. Many morphological and behavioral characteristics of N. femorata are known to be affected by cactus phenology in a controlled laboratory setting, inclu...

  5. Dopluise (Hemiptera: Coccoidea) geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae)

    OpenAIRE

    Johannes H. Giliomee

    2015-01-01

    Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae), is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea) in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise), Coccidae (sagtedopluise) en Kerriidae (lakdopluise) is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhoudi...

  6. Scale insect genus-group names and their families (Hemiptera: Sternorrhyncha: Coccoidea).

    Science.gov (United States)

    Williams, Douglas J; Denno, Barbara D

    2014-06-05

    Genus names of the scale insects (Hemiptera: Coccoidea) are listed with their current families. The list includes all generic names that are currently valid with synonyms, unjustified emendations, homonyms, nomia nuda and misspellings. The list has been compiled from many sources as discussed in the introduction. This is the first list to be prepared in recent years of all generic names and it is in alphabetical order.

  7. Novas Plantas Hospedeiras para o Percevejo Fitófago Leptoglossus zonatus (Dallas (Hemiptera: Coreidae

    Directory of Open Access Journals (Sweden)

    Evaldo Pires

    2012-12-01

    Abstract. Leptoglossus zonatus (Dallas (Hemiptera: Coreidae is a phytophagous bug that can be found in several crops due to availability of food and favorable conditions for the development. The aim of this research was to record the occurrence of L. zonatus colonizing plants of Malpighia emarginata Sessé & Moc (Malpighiaceae, Morus nigra Linnaeus (Moraceae, Mangifera indica Linnaeus (Anacardiaceae in Municipality of Sinop, Mato Grosso, Brazil. L. zonatus should be included in monitoring for the Integrated Pest Management (IPM in these plants.

  8. Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

    OpenAIRE

    GOMEZ MARCO, FRANCESC

    2016-01-01

    [EN] Aphis spiraecola Patch. (Hemiptera: Aphididae) is a key pest of clementines. Biological control of A. spiraecola is still poorly known and efforts were based on the use and conservation of parasitoids but it did not success. With all this said, the aims of this thesis were: i) to disentangle the reasons behind the low parasitism of A. spiraecola; ii) to determine when and how predators can control A. spiraecola populations; and, finally, iii) to evaluate whether a ground cover of Poaceae...

  9. Aglaenita (Hemiptera: Cicadellidae: Neocoelidiinae: a new species from Brazil

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    Ana Paula Marques-Costa

    2011-02-01

    Full Text Available Aglaenita Spinola, 1850 comprises fourteen species, all occurring in Brazil, including a new species, A. hambletoni sp. nov., which is described herein. The genus is close to Biza Walker, 1858 and Megacoelidia Kramer & Linnavuori, 1959. Aglaenita hambletoni sp. nov. can be distinguished from other Aglaenita species mainly by the characters of male genitalia, especially by the pygofer bearing an apical bifurcated process, the branches of the process pointed and sclerotized, the upper branch longer than the lower, tooth-like one; and by the aedeagus without processes and with the ventral margin without teeth. The diagnosis of the genus and the known geographical distribution of its species are also given

  10. Influence of insecticide treatments on ant-hemiptera associations in tropical plantations.

    Science.gov (United States)

    Kenne, M; Djiéto-Lordon, C; Orivel, J; Mony, R; Fabre, A; Dejean, A

    2003-04-01

    In this survey conducted in southern Cameroon, we compared ant-Hemiptera associations on plantations treated with insecticides, on plantations 2 years after insecticide treatments ceased, and on control lots that never received insecticide treatments. By eliminating arboreal-nesting ants, insecticides favored the presence of "ecologically dominant" ground-nesting, arboreal-foraging species that occupied the tree crowns. The reinstallation of arboreal ants was slow as 2 yr after insecticide treatment ceased differences with the control lots were significant. This intermediary period also illustrated that arboreal ants can found and develop colonies on trees occupied by ground-nesting species. Certain arboreal species were more frequent during this intermediary period than on the control lots, showing that the period of installation in the trees was followed by competition between arboreal ants. We confirm that ground-nesting ants tend a wide range of hemipteran families, including well known agricultural pests, whereas arboreal ants, particularly dominant species, were mostly associated with Coccidae and Stictococcidae that do not pose problems to the supporting trees. A tree effect was also noted for both ant and hemipteran distribution. We concluded that because of insecticide use, ground-nesting ants pose problems through their associated Hemiptera. On the contrary, dominant arboreal ants, strong predators, benefit their supporting trees by excluding ground-nesting species and tending mostly nonpest Hemiptera. Nevertheless, certain of them, carpenter species or species likely to tend Pseudococcidae, have to be eliminated through integrated management.

  11. Identification and impact of natural enemies of Bactericera cockerelli (Hemiptera: Triozidae) in Southern California.

    Science.gov (United States)

    Butler, Casey D; Trumble, John T

    2012-10-01

    Bactericera cockerelli (Sulc) (Hemiptera: Triozidae) is a major pest of potato, (Solanum tuberosum L.), tomato (Solanum lycopersicum L.), and peppers (Capsicum spp.). The purpose of our research was to identify and determine the impact of natural enemies on B. cockerelli population dynamics. Through 2 yr of field studies (2009-2010) at four different sites and laboratory feeding tests, we identified minute pirate bug, Orius tristicolor (White) (Hemiptera: Anthocoridae); western bigeyed bug, Geocoris pallens Stål (Hemiptera:Geocoridae), and convergent lady beetle, Hippodamia convergens Guérin-Méneville (Coleoptera: Coccinellidae) as key natural enemies of B. cockerelli in southern California potatoes, tomatoes, and bell peppers. In natural enemy exclusion cage experiments in the potato crop and in American nightshade, Solanum americanum Miller, the number of B. cockerelli surviving was significantly greater in the closed cage treatments, thus confirming the affect natural enemies can have on B. cockerelli. We discuss how this information can be used in an integrated pest management program for B. cockerelli.

  12. Fingeriana dubia gen. nov. e sp. nov. de Cicadellini (Hemiptera, Auchenorrhyncha, Cicadellidae do sudeste e sul do Brasil Fingeriana dubia gen. nov. and sp. nov. of Cicadellini (Hemiptera, Auchenorrhyncha, Cicadellidae from Southeast and South of Brazil

    Directory of Open Access Journals (Sweden)

    Rodney Ramiro Cavichioli

    2003-06-01

    Full Text Available New genus and new species of Cicadellini are proposed: Fingeriana dubia occurs at the citrus and coffee culture. The new genera is similar with Nielsonia Young, 1977 but, the shaft of the adeagus of the Fingeriana dubia is symmetrical with a basal unpaired process asymmetrical and plates narrowly triangular as long as pygofer.

  13. Two new Nirvanini genera from China (Hemiptera: Cicadellidae).

    Science.gov (United States)

    Gao, Min; Dai, Wu; Zhang, Yalin

    2014-01-01

    Two new Nirvanini genera, Decursusnirvana gen. nov. (type species: Decursusnirvana fasciiformis sp. nov.) and Sinonirvana gen. nov. (type species: Sinonirvana hirsuta sp. nov.), including two new species, D. fasciiformis sp. nov. and S. hirsuta, sp. nov. from China are described. One new combination, Decursusnirvana excelsa (Melichar) n. comb., is also proposed. Decursusnirvana most closely resembles Oniella Matsumura, but it may be distinguished from the latter by the aedeagus lacking an atrium and with the shaft with its basal half straight and apical half strongly curved ventrally, with a pair of processes. Sinonirvana gen. nov is similar to Decursusnirvana gen. nov., differs from the latter as follows: head more elongate with anteclypeus much broader and not extended beyond margin of gena. Also, the pygofer lobe is solid (not divided in two places like the latter) and the subgenital plate has numerous long, fine setae. Detailed morphological descriptions and illustrations of all three species are given and a key to all male species in two genera is also provided. 

  14. Two new species of mealybugs (Hemiptera, Coccoidea, Pseudococcidae from Iran

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    Masumeh Moghaddam

    2010-04-01

    Full Text Available Polystomophora arakensis Moghaddam and Phenacoccus salviacus Moghaddam are described and illustrated in detail from Iran. The species P. arakensis was collected from the roots of Atraphaxis sp. (Family: Polygonaceae and P. salviacus was collected from the leaves of Salvia bracteata (Family: Labiatae. The genera Phenacoccus and Polystomophora are briefly reviewed and a key to the Iranian species of Phenacoccus is provided.

  15. Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae from Colombia

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    Ana Milena Caicedo

    2011-05-01

    Full Text Available A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein described using morphological and molecular data. It was found parasitising the alimentary tract of the insect host Cyrtomenus bergi, a polyphagous pest. The morphology of B. cyrtomeni was investigated using light and transmission microscopy and molecular phylogeny was inferred from the sequences of spliced leader RNA (SL rRNA - 5S rRNA gene repeats and the 18S small subunit (SSU rRNA gene. Epimastigotes of variable size with straphanger cysts adhering to the middle of the flagellum were observed in the intestinal tract, hemolymph and Malpighian tubules. Kinetoplasts were always observed anterior to the nucleus. The ultrastructure of longitudinal sections of epimastigotes showed the flagellum arising laterally from a relatively shallow flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats were positive in all cases, producing a 0.8-kb band. The amplicons were 797-803 bp long with > 98.5% identity, indicating that they originated from the same organism. According to the sequence analysis of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni is different from all other known species or isolates of Trypanosomatidae. Both analyses indicate that among known species, it is most closely related to Blastocrithidia triatomae.

  16. Acetobacter intermedius, sp. nov.

    Science.gov (United States)

    Boesch, C; Trcek, J; Sievers, M; Teuber, M

    1998-03-01

    Strains of a new species in the genus Acetobacter, for which we propose the name A. intermedius sp. nov., were isolated and characterized in pure culture from different sources (Kombucha beverage, cider vinegar, spirit vinegar) and different countries (Switzerland, Slovenia). The isolated strains grow in media with 3% acetic acid and 3% ethanol as does A. europaeus, do, however, not require acetic acid for growth. These characteristics phenotypically position A. intermedius between A. europaeus and A. xylinus, DNA-DNA hybridizations of A. intermedius-DNA with DNA of the type strains of Acetobacter europaeus, A. xylinus, A. aceti, A. hansenii, A. liquefaciens, A. methanolicus, A. pasteurianus, A. diazotrophicus, Gluconobacter oxydans and Escherichia coli HB 101 indicated less than 60% DNA similarity. The important features of the new species are described. Acetobacter intermedius strain TF2 (DSM11804) isolated from the liquid phase of a tea fungus beverage (Kombucha) is the type strain.

  17. Taxonomic study of the leafhopper genus Thagria Melichar (Hemiptera: Cicadellidae: Coelidiinae) from Guangxi, China.

    Science.gov (United States)

    Fan, Zhi-Hua; Li, Zi-Zhong; Dai, Ren-Huai

    2015-01-01

    The paper deals with 21 valid species of the genus Thagria from Guangxi Autonomous Region, China. Eight new species are described and illustrated: T. biprocessa Fan & Dai, sp. nov., T. decussata Fan & Dai, sp. nov., T. irregularis Fan & Dai, sp. nov., T. multispinosa Fan & Dai, sp. nov., T. paramultipars Fan & Li, sp. nov., T. triangula Fan & Li, sp. nov., T. trifasciata Fan & Li, sp. nov. and T. webbi Fan & Li, sp. nov.. A key is given to distinguish all species of this genus from Guangxi, China and maps showing the geographic distribution of new species are also provided. The name Thagria xuae nom. emend. is given for T. xui Nielson.

  18. Nueva especie del género Ambrysus (Hemiptera: Naucoridae

    Directory of Open Access Journals (Sweden)

    Mónica LÓPEZ RUF

    2007-01-01

    Full Text Available Se describe e ilustra Ambrysus (Ambrysus calilegua sp. nov. de la Argentina, y se compara con A. (A. kolla López Ruf. Se describe el corion del huevo de A. calilegua y se ilustra con fotografías del MEB. El corion es de carácter taxonómico y muestra claras diferencias interespecíficas en el género Ambrysus (Stål.

  19. Artrópodes fitófagos e predadores associados em 20 acessos Luffa sp. em sistema orgânico Phytophagous and predators arthropods associated in 20 accesses of Luffa sp in organic system

    Directory of Open Access Journals (Sweden)

    Germano Leão Demolin Leite

    2011-12-01

    Full Text Available Os objetivos desta pesquisa foram verificar os artrópodes fitófagos e seus predadores em 20 acessos de bucha vegetal Luffa sp. (Cucurbitaceae, a distribuição destes no dossel das plantas em cultivo orgânico e a produção de frutos dos acessos. O experimento foi em blocos casualizados com 20 tratamentos e quatro repetições. Os tratamentos foram os acessos P159, P304, P466, P03, P570, P569, P467, P30, P446, P495, P125, P263, P644 e P32, provenientes da Embrapa (CENARGEN, DF, e P01, P02, P04, P05, P06 e P07, obtidos em Porteirinha-MG. As parcelas foram compostas por quatro plantas de Luffa sp. A maior abundância de Diabrotica speciosa (Germar (Coleoptera: Chrysomelidae foi observada nas folhas do acesso P467 em referência aos demais acessos de Luffa sp. Trigona spinipes (Fabricius (Hymenoptera: Apidae foi mais observada nas flores dos acessos P01, P03, P30, P32, P263 e P569 e menos abundantes nos acessos P05, P125, P159, P304, P446 e P570. Os acessos de Luffa sp. com maior número de frutos/planta foram P01, P03, P05, P06, P07, P30, P32, P125, P263, P446 e P570. Os predadores aranhas e Coccinellidae (Coleoptera bem como o fitófago Cicadellidae (Hemiptera preferiram atacar a face adaxial, sendo que não se detectou diferença estatística entre as faces para os demais artrópodes. T. spinipes esteve mais presente na parte apical do dossel das plantas. Com esses resultados, o acesso P06 é o mais indicado para o cultivo, pois foi um dos que apresentou maiores produtividades e menores taxas de ataque de insetos.The objectives of this research were to verify the phytophagous arthropods and their predators in 20 accesses of vegetable loofah Luffa sp. (Cucurbitaceae, the distribution of the canopy of the plants in organic system and the production of fruits of the accesses. The experiment was in randomized blocks designed with 20 treatments and four replicates. The treatments were the accesses P159, P304, P466, P03, P570, P569, P467, P30, P446

  20. New species of the treehopper tribe Amastrini (Hemiptera, Membracidae, Smiliinae Novas espécies de membracídeos da tribo Amastrini (Hemiptera, Membracidae, Smiliinae

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    Olívia Evangelista

    2007-03-01

    Full Text Available Three new species of Amastris Stål and one of Erosne Stål are described and illustrated. Amastris rotheai sp. nov. (Paraiba, Brazil, A. gibberula sp. nov. (Mato Grosso, Brazil, A. comarapa sp. nov. (Santa Cruz, Bolivia, and Erosne parvula sp. nov. (Paraiba, Brazil.Três novas espécies de Amastris Stål e uma de Erosne Stål são descritas e ilustradas. Amastris rotheai sp. nov. (Paraíba, Brasil, A. gibberula sp. nov. (Mato Grosso, Brasil, A. comarapa sp. nov. (Santa Cruz, Bolívia e Erosne parvula sp. nov. (Paraíba, Brasil.

  1. Eriocóccidos (Hemiptera: Coccoidea encontrados en Larrea spp. (Zygophyllaceae en Argentina Eriococcidae (Hemiptera: Coccoidea found on Larrea spp. (Zygophyllaceae in Argentina

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    Patricia GONZÁLEZ

    2009-06-01

    Full Text Available Se describen e ilustran las hembras adultas de cuatro especies nuevas de eriocóccidos: Eriococcus clapsae sp. nov., E. cuneifoliae sp. nov., E. divaricatae sp. nov. y E. pituilensis sp. nov. Ovaticoccus lahillei (Leonardi se transfiere al género Eriococcus como E. lahillei (Leonardi, comb. nov., su hembra adulta y su primer estadio ninfal son redescriptos e ilustrados. Todas las especies de eriocóccidos arriba mencionados ocurren en Argentina y se encuentran en plantas del género Larrea.Four new eriococcid species are described and illustrated in the present paper: Eriococcus clapsae sp. nov., E. cuneifoliae sp. nov., E. divaricatae sp. nov. and E. pituilensis sp. nov,. Ovaticoccus lahillei (Leonardi is transferred to the genus Eriococcus as E. lahillei (Leonardi, comb. nov., and its adult female and first-instar nymph are redescribed and illustrated. All five eriococcid species occur in Argentina and are found on plants of the genus Larrea.

  2. Morphology of the female reproductive system and physiological age-grading of Megamelus scutellaris (Hemiptera: Delphacidae), a biological control agent of water hyacinth

    Science.gov (United States)

    The morphology of the female reproductive system in Megamelus scutellaris Berg (Hemiptera:Delphacidae), a biocontrol agent of Eichhornia crassipes (Mart.) Solms, was examined using standard light microscopy techniques. Ovaries extracted from individuals dissected in phosphate buffered saline were ex...

  3. Morphology of the first-instar nymph and adult female of Kermes echinatus Balachowsky, with a comparison to K. vermilio Planchon (Hemiptera, Coccoidea, Kermesidae

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    Malkie Spodek

    2012-11-01

    Full Text Available The first-instar nymph and the adult female of Kermes echinatus Balachowsky (Hemiptera, Coccoidea, Kermesidae are described and illustrated. This species is compared with K. vermilio Planchon, a morphologically similar species known in the Palaeractic region.

  4. Potential use of the fungus Beauveria bassiana against the western flower thrips Frankliniella occidentalis without reducing the effectiveness of its natural predator Orius sauteri (Hemiptera: Anthocoridae)

    Science.gov (United States)

    Orius sauteri (Poppius) (Hemiptera: Anthocoridae) is an important predator of western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Orius sauteri would be directly exposed to the entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin in the field should the fu...

  5. Biza Walker: cinco espécies novas do Brasil (Hemiptera, Cicadellidae, Neocoelidiinae Biza Walker: five new Brazilian species (Hemiptera, Cicadellidae, Neocoelidiinae

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    Larissa de Bortolli Chiamolera

    2003-06-01

    Full Text Available In the present study five new species of Biza are presented: B. castanea sp. nov., B. ocellata sp. nov., B. maculata sp. nov., B. trimaculata (all from Brazil, Amazonas State and B. similis sp. nov. (from Brazil, Mato Grosso State. The new species of Biza can be distinguished by the aspect of the male genitalia, mainly by the shape of the aedeagus, the presence or absence of process in the aedeagus and shape of pygofer.

  6. Roseomonas terrae sp. nov.

    Science.gov (United States)

    Yoon, Jung-Hoon; Kang, So-Jung; Oh, Hyun Woo; Oh, Tae-Kwang

    2007-11-01

    A Gram-negative, non-motile, coccobacilli-shaped bacterium, DS-48T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-48T grew optimally at 25 degrees C and pH 7.0-8.0 in the presence of 0.5% (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C18:1omega7c and C18:1 2-OH as the major fatty acids. The DNA G+C content was 69.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-48T fell within the genus Roseomonas, clustering with Roseomonas lacus TH-G33T (at a bootstrap confidence level of 100%). The levels of similarity between the 16S rRNA gene sequence of strain DS-48T and those of the type strains of recognized Roseomonas species were in the range 93.2-98.0%. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness of DS-48T, revealed that this strain differs from recognized Roseomonas species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain DS-48T represents a novel species within the genus Roseomonas, for which the name Roseomonas terrae sp. nov. is proposed. The type strain is DS-48T (=KCTC 12874T=JCM 14592T).

  7. Bradysia sp. em morangueiro Bradysia sp. in strawberry

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    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  8. Biological parameters and thermal requirements of the parasitoid Praon volucre (Hymenoptera: Braconidae) with Macrosiphum euphorbiae (Hemiptera: Aphididae) as host

    NARCIS (Netherlands)

    Conti, De B.F.; Bueno, V.H.P.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    The effect of temperature on the biology of Praon volucre (Haliday, 1833) (Hymenoptera: Braconidae) in Macrosiphum euphorbiae (Thomas, 1878) (Hemiptera: Aphididae) hosts was studied and the thermal requirements of the parasitoid were determined. Experiments were carried out at 16, 19, 22, 25, and 28

  9. New corological and biological data of the Red Gum Lerp Psyllid, Glycaspis brimblecombei Moore, 1964 in Italy (Hemiptera, Psyllidae

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    Ricardo Jiménez-Peydró

    2011-03-01

    Full Text Available Glycaspis brimblecombei Moore, 1964 is a psyllid (Hemiptera: Psyllidae pest of Eucalyptus, native toAustralia and first recorded in Europe: Spain in 2008 and more recently (2010 in Italy. The present paper dealswith recent research, carried out in central Italy, with new data on the distribution and biology of this species.

  10. Brochosome influence on parasitisation efficiency of Homalodisca coagulata (Say) (Hemiptera: Cicadellidae) egg masses by Gonatocerus ashmeadi Girault (Hymenoptera : Mymaridae)

    NARCIS (Netherlands)

    Velema, H.P.; Hemerik, L.; Hoddle, M.S.; Luck, R.F.

    2005-01-01

    1. Many cicadellid females in the tribe Proconiini (Hemiptera: Cicadellidae) cover their egg masses with specialised, usually rod-shaped, brochosomes as the eggs are being laid. The brochosomes are produced in Golgi complexes in the Malpighian tubules of Cicadellidae. In contrast to the gravid femal

  11. Review of the grassland leafhopper genus Nephotettix Matsumura (Hemiptera: Cicadellidae: Deltocephalinae: Chiasmini) from the Chinese mainland.

    Science.gov (United States)

    Duan, Yani; Zhang, Yalin

    2014-01-01

    The Chinese mainland species of the leafhopper genus Nephotettix Matsumura (Hemiptera: Cicadellidae: Deltocephalinae: Chiasmini) (Nephotettix cincticeps (Uhler), Nephotettix malayanus Ishihara & Kawase, Nephotettix nigropictus (Stål), Nephotettix parvus Ishihara & Kawase rec. n., and Nephotettix virescens (Distant)) are reviewed. This genus is redescribed. Variation is described and illustrated. Keys for their separation are provided together with a worldwide checklist.

  12. Evaluation of a method to quantify glassy-winged sharpshooter (Hemiptera: Cicadellidae) egg maturation during a feeding assay

    Science.gov (United States)

    Methods to improve an assay relating adult feeding to egg maturation by the glassy-winged sharpshooter (Hemiptera: Cicadellidae) were evaluated. The assay consisted of confining adult females to cowpea stems in parafilm enclosures and quantifying adult feeding and egg maturation. Adult feeding was...

  13. A laboratory study of sex- and stage-related mortality and morbidity in bed bugs (hemiptera: cimicidae) exposed to deltamethrin

    Science.gov (United States)

    Exposure of a pyrethroid-susceptible strain of bed bugs, Cimex lectularius L. (Hemiptera: Cimicidae) to varying concentrations of deltamethrin for 24h indicated there was no significant difference in mortality between males, females, and nymphs at 24h or 168h post-exposure. Most bed bugs classified ...

  14. Potential transmission of Pantoea spp. and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) to plants by Lygus hesperus (Hemiptera: Miridae)

    Science.gov (United States)

    Lygus hesperus Knight (Hemiptera: Miridae) is a key agricultural pest in the western United States. In a recent study, proteins from Pantoea ananatis and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) were identified in diet that was stylet-probed and fed upon by L. hesperus adults. P...

  15. Eriosomatine aphids (Hemiptera, Aphididae, Eriosomatinae) associated with moss and roots of conifer and willow in forests of the Pacific Northwest

    Science.gov (United States)

    Apterous adult morphs of eriosomatine aphids (Hemiptera, Aphididae, Eriosomatinae) associated with moss and/or roots of conifer or willow in forests of the Pacific Northwest including Alaska are described, illustrated, and keyed. In total, seven species (Clydesmithia canadensis Danielsson, Melaphis ...

  16. Survey of 'Candidatus Liberibacter solanacearum' in carrot crops affected by the psyllid Trioza apicalis (Hemiptera: Triozidae) in Norway

    Science.gov (United States)

    The carrot psyllid Trioza apicalis Förster (Hemiptera: Triozidae) is a serious insect pest of carrot (Daucus carota L.) in northern Europe, where it can cause up to 100% crop loss. Although it was long believed that T. apicalis causes damage to carrot by injection of toxins into the plant, it was re...

  17. Four new species of Alebroides Matsumura (Hemiptera: Cicadellidae: Typhlocybinae) from China .

    Science.gov (United States)

    Yu, Xiaofei; Yang, Maofa

    2014-01-01

    Four new species of the empoascine leafhopper genus Alebroides Matsumura are reported from China: A. strumae Yu & Yang, sp. nov., A. chiasmaticus Yu & Yang, sp. nov., A. serrulatus Yu & Yang, sp. nov. and A. spanner Yu & Yang, sp. nov. A list of all Chinese species of the genus is provided.

  18. Predators of the Alfalfa Aphids Acyrthosiphon pisum (Harris, Aphis craccivora Koch, and Therioaphis trifolii (Monell (Hemiptera: Aphidoidea as Determined by the Serological Technique

    Directory of Open Access Journals (Sweden)

    Suzan Cunha

    2016-08-01

    Resumo. A serologia é uma técnica imunológica baseada em reações antígeno/anticorpo, em que suas principais vantagens são a alta sensibilidade e especificidade que permitem o reconhecimento biológico em nível molecular. Este trabalho avalia o uso da técnica serológica para determinar os predadores dos pulgões da alfafa, Acyrthosiphon pisum (Harris, Aphis craccivora Koch e Therioaphis trifolii (Monell (Hemiptera: Aphidoidea. As coletas dos pulgões para a obtenção dos anticorpos e de seus possíveis predadores para serem utilizados como antígenos foram realizadas nos campos de alfafa da Unidade da Embrapa Pecuária Sudeste, São Carlos, SP. no período de agosto de 2011 a julho de 2012. Foram testados 2.161 artrópodes predadores, incluindo insetos e aranhas. Os anticorpos obtidos para os pulgões A. craccivora, A. pisum, e T. trifolii mostraram identidade parcial mas, ainda assim, permitiu reconhecer os predadores dos pulgões da alfafa. Dentre os insetos, sirfídeos e crisopídeos foram os que apresentaram as maiores porcentagens de resultados positivos nos testes serológicos. A. craccivora foi o afídeo mais consumido pelos predadores.

  19. Does cycad aulacaspis scale (Aulacaspis yasumatsui, Hemiptera: Diaspididae) play a direct role in causing soil phytotoxicity?

    Science.gov (United States)

    Watson, Gillian; Marler, Thomas E

    2014-01-01

    Cycad aulacaspis scale (CAS, Aulacaspis yasumatsui, Hemiptera: Diaspididae) was accidentally introduced to Guam in 2003, and has caused acute mortality of the dominant, endemic forest tree Cycas micronesica. A phytotoxic legacy in the soils beneath cycad trees killed by CAS over a period of about three years has been demonstrated. The origin of the toxicity may be large quantities of CAS-encrusted cycad leaf litter. We explore the possibility that a major contribution to this toxic legacy may come from the scale insects, not just from the plant material.

  20. Dopluise (Hemiptera: Coccoidea geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae

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    Johannes H. Giliomee

    2015-02-01

    Full Text Available Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae, is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise, Coccidae (sagtedopluise en Kerriidae (lakdopluise is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhouding tussen die miere en dopluise, bekend as mirmekofilie, is fakultatief van aard. Die wipstertmier blyk ook nie spesifiek te wees wat betref die plant waarop hulle nes maak nie.

  1. First report of Idiopterus nephrelepidis Davis, 1909 (Hemiptera: Aphididae from Bulgaria

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    Elena Tasheva-Terzieva

    2011-11-01

    Full Text Available Idiopterus nephrelepidis (Hemiptera: Aphididae is reported for the first time in Bulgaria on ornamental ferns in four greenhouses in Sofia and Varna. Dense colonies of apterous viviparous females and larvae were observed. The established host plants are Nephrolepis exaltata, Asplenium nidus and Pteris cretica. Infested ferns exhibit leaf deformation. The aphids were reared in laboratory conditions for four months. A morphometric study of apterae was carried out. Taking into account the presence of host plants of I. nephrelepidis in Bulgaria which are native to the local flora and the reports of the aphid from the Balkan area, it may spread in the country outdoors.

  2. Records of Natural Enemies of Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) Biotype B in Brazil.

    Science.gov (United States)

    Torres, L C; Lourenção, A L; Costa, V A; Souza, B; Costa, M B; Tanque, R L

    2014-04-01

    Collections of natural enemies of Bemisia tabaci biotype B (Genn.) (Hemiptera: Aleyrodidae) were made in Lavras, state of Minas Gerais, Brazil. In the greenhouse, 6,495 predators and 16,628 parasitoids belonging to three families were collected. In the field, 267 predators and 344 parasitoids belonging to five families were found. For the first time in Brazil, five species of predators associated with this whitefly were reported. Because of the diversity of natural enemies of B. tabaci biotype B recorded, this study points out the importance of these data for studies on integrated pest management.

  3. The genus Arctorthezia Cockerell (Hemiptera, Ortheziidae with the description of a new species

    Directory of Open Access Journals (Sweden)

    Éva Szita

    2015-01-01

    Full Text Available This paper describes a new species of Arctorthezia Cockerell (Hemiptera: Coccoidea: Ortheziidae from the Palaearctic region. The specimens were extracted from forest litter in the collections of Muséum d’histoire Naturelle de Genève, Switzerland, using Berlese funnels. Three further species, A. cataphracta (Olafsen, A. occidentalis (Douglas and A. pseudoccidentalis Morrison, are redescribed and re-illustrated. The genus Arctorthezia now contains five species. An identification key, diagnostic illustrations, photographs of unmounted females and new locality records of the Arctorthezia species currently known are provided.

  4. The Vine Mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) Damaging Vineyards in Brazil.

    Science.gov (United States)

    Pacheco da Silva, V C; Galzer, E C W; Malausa, T; Germain, J F; Kaydan, M B; Botton, M

    2016-08-01

    In the last decade, the incidence of mealybugs (Hemiptera: Pseudococcidae) in vineyards has increased, especially on crops grown under plastic covering, in the Serra Gaúcha region of southern Brazil where the major Brazilian wineries are concentrated. Eggs, nymphs, and female adults were collected in two highly infested vineyards in Bento Gonçalves City, Rio Grande do Sul, Brazil. Mealybugs were identified by morphological and molecular techniques as the vine mealybug Planococcus ficus (Signoret). This is a principal mealybug pest of vineyards worldwide, and this is the first record of damage from this species in Brazil.

  5. [First record of Platycorypha nigrivirga Burckhardt (Hemiptera: Psylloidea), in Tipuana tipu (Benth.), from Brazil].

    Science.gov (United States)

    Santana, Dalva L Q; Burckhardt, Daniel; Aguiar, António M F

    2006-01-01

    The occurrence of Platycorypha nigrivirga Burckhardt (Hemiptera: Psyllidae, Acizzinae), is reported for the first time, in Brazil. The psyllid was observed causing injuries on leaves and shoots of Tipuana tipu (Benth.), in urban areas of Paraná State. P. nigrivirga are tiny sap-sucking insects that feed on phloem and cause curling, stains and fall of leaves. The excrements of these insects accumulate on the leaves propitiating fungus growth on the leaves, or they fall in great amount on the cars dirtying them as if they were sparkling of white and sticky ink.

  6. First record of Diaphorina citri (Hemiptera: Psyllidae) in Ecuador infesting urban citrus and orange jasmine trees.

    Science.gov (United States)

    Cornejo, J F; Chica, E J

    2014-01-01

    Adults and nymphs of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), were collected in the Guayaquil, Samborondón, and Durán cantons in coastal Ecuador. Psyllids were found in high numbers in citrus (Citrus spp., Sapindales: Rutaceae) and orange jasmine (Murraya exotica [L.] Jack, Sapindales: Rutaceae) trees within the Guayaquil-Samborondon-Duran conurbation; however, none was found during scoutings in the main citrus producing areas in coastal Ecuador. To the best of our knowledge, this is the first report of D. citri in Ecuador and the Pacific coastal plain of South America.

  7. Cicadidae types (Hemiptera-Cicadomorpha) housed at the Museo de La Plata entomological collection (Argentina).

    Science.gov (United States)

    De Remes Lenicov, Ana M Marino; Maciá, Arnaldo; Pianzola, Bruno

    2015-06-23

    A catalog of the 161 type specimens of species of Hemiptera Cicadidae housed in the collection of the Entomology Division of the Museo de La Plata is presented. This collection represents 52 species grouped in 19 genera. For each species the original and current names, bibliographic references, type category, number of specimens, gender, Museo de La Plata code numbers, and transcription of data from labels (country, province, locality, date of collection, collector's name, and hosts) are given. Information about the state of preservation of the specimens in each series and photographs of each type species are also provided.

  8. Actinoplanes couchii sp. nov.

    Science.gov (United States)

    Kämpfer, Peter; Huber, Birgit; Thummes, Kathrin; Grün-Wollny, Iris; Busse, Hans-Jürgen

    2007-04-01

    A Gram-positive bacterium, strain GW8-1761(T), was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes italicus JCM 3165(T) (98.9 %), A. rectilineatus IFO 13941(T) (98.5 %), A. palleronii JCM 7626(T) (97.8 %), A. utahensis IFO 13244(T) (97.6 %) and A. cyaneus DSM 46137(T) (97.6 %). Strain GW8-1761(T) could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H(4)); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C(15 : 0), C(16 : 0), C(16 : 0) iso, C(17 : 1)omega8c and summed feature 3 (C(16 : 1)omega7c and/or C(15 : 0) iso 2-OH)] supported the affiliation of strain GW8-1761(T) to the genus Actinoplanes. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761(T) from the most closely related species. Strain GW8-1761(T) therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761(T) (=DSM 45050(T)=CIP 109316(T)).

  9. Arcobacter marinus sp. nov.

    Science.gov (United States)

    Kim, Hye Min; Hwang, Chung Yeon; Cho, Byung Cheol

    2010-03-01

    A slightly curved, rod-shaped marine bacterium, designated strain CL-S1(T), was isolated from near Dokdo, an island in the East Sea, Korea. Cells were Gram-negative and grew well under either aerobic or microaerobic conditions. Analyses of the 16S rRNA and gyrA gene sequences of strain CL-S1(T) revealed an affiliation with the genus Arcobacter within the class Epsilonproteobacteria. Phylogenetic analyses based on 16S rRNA and gyrA gene sequences showed that strain CL-S1(T) formed a robust clade with Arcobacter halophilus LA31B(T), with sequence similarities of 96.1 and 88.2 %, respectively. DNA-DNA relatedness between strain CL-S1(T) and A. halophilus DSM 18005(T) was 44 %, indicating that they represent genomically distinct species. Strain CL-S1(T) grew optimally at 30-37 degrees C, at pH 7 and in the presence of 3-5 % NaCl. The dominant cellular fatty acids were iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (28.4 %), C(16 : 0) (26.2 %) and C(18 : 1)omega7c (22.3 %). The DNA G+C content of strain CL-S1(T) was 28 mol%. Strain CL-S1(T) differed phenotypically from A. halophilus LA31B(T) based on its ability to grow aerobically at 10 degrees C and inability to grow under anaerobic conditions. Based on the data presented, strain CL-S1(T) is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter marinus sp. nov. is proposed. The type strain is CL-S1(T) (=KCCM 90072(T) =JCM 15502(T)).

  10. Placoscopana, a new genus of Neocoelidiinae (Hemiptera: Cicadellidae from Ecuador

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    Clayton Correa Gonçalves

    2012-10-01

    Full Text Available A new genus of Neocoelidiinae, Placoscopana gen. nov. is proposed and its type-species Placoscopana nigrilinea sp. nov. is described and illustrated based on a male specimen from Ecuador (Orellana Province. Placoscopana gen. nov. is very similar externally to Coronalidia Marques-Costa & Cavichioli, 2007, but differs mainly by the male genitalia: pygopher short with posterior margin approximately truncated; subgenital plates, in dorsal view, with a tuft of setae at apex; and aedeagus with two parallel ventral rows of small spines on the apical half of the shaft.

  11. A new species of Portanus (Hemiptera, Cicadellidae, Xestocephalinae from Brazil

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    Adenomar N. de Carvalho

    2012-12-01

    Full Text Available Portanus Ball, 1932 comprises 45 species that occur in Brazil, including Portanus felixi sp. nov. described and illustrated herein. The genus is close to Paraportanus Carvalho & Cavichioli, 2009 and can be distinguished from it by having a transversal groove on the basal third of the subgenital plates. The new species can be distinguished from the other species of the genus by the characters of male genitalia, especially by the pygofer with the apical process pointed, sclerotized and dorso-ventrally directed; and by the aedeagus with apodeme on the basal third.

  12. The structure of integument and wax glands of Phenacoccus fraxinus (Hemiptera: Coccoidea: Pseudococcidae)%The structure of integument and wax glands of Phenacoccus fraxinus (Hemiptera:Coccoidea:Pseudococcidae)

    Institute of Scientific and Technical Information of China (English)

    Yanfeng ZHANG; Yingping XIE; Jiaoliang XUE; Xiaohong FU; Weimin LIU

    2012-01-01

    Using scanning electron microscopy and optical microscopy,we studied the structure of the integument and wax glands of the mealybug,Phenacoccus fraxinus Tang (Hemiptera:Coccoidea:Pseudococcidae).We observed the ultrastructure of four wax pores including trilocular,quinquelocular,and multilocular pores as well as tubular ducts,recording characteristics of their structure,size and distribution.We found that that the integument of the mealybug consists of three main layers—the procuticle,epidermis and basement membrane—and four sub-layers of the procuticle—the epicuticle,exocuticle,endocuticle and formation zone.The waxsecreting gland cells were closely arranged in epidermis.All of them were complex and composed of one central cell and two or more lateral cells.These complex cells possess a large common reservoir for collection and storage.Synthesized by the glandular cells,the wax is excreted outside integument through canals.

  13. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

    DEFF Research Database (Denmark)

    Houbraken, Jos; López-Quintero, Carlos A.; Frisvad, Jens Christian

    2011-01-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178T = IBT 23262T), Penicillium wotroi sp. nov. (type strain CBS 118171T = IBT 23253T...

  14. Biodiversidade e Sazonalidade de Cigarrinhas (Hemiptera: Membracidae em Cacaueiros

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    Vera Benassi

    2016-04-01

    Abstract. The aim of this study was to identify the treehoppers diversity associated with cocoa plantation and to determine the seasonality of the most common species in two shaded areas, in the municipality of Linhares, Espirito Santo State, Brazil. For the sampling, twelve yellow-tray traps were randomly set in each area, spaced from one another in 30 m., placed on wooden stakes at a height of 1.0 m. above ground level. Weekly the collected material was taken during August/2011 to July/2012. Also samples of fruits, branches and leaves containing postures, nymphs, and adults of the treehoppers were collected and maintained in the laboratory for the eventual emergence of parasitoids and cataloging of the species. Through the traps were collected 16,094 exemplars of treehoppers belonging to 30 species, of which, 58.7 % in the first area (70 % shade and 41.3 % in the second area (60 % shade. The most abundant species was Horiola picta (Coquebert comprising 87.2 % of the total collection. Cocoa trees are confirmed to be the host plant of H. picta, Membracis tectigera Olivier, Membracis dorsata Fabricius, Membracis mimica Walker, Anobilia sp., Phormophora maura (Fabricius, Leioscyta pulchella Funkhouser and Enchenopa squamigera (Linnaeus. Parasitoids belonging to the families Mymaridae and Trichogrammatidae (Hymenoptera were obtained from the eggs of H. picta, Anobilia sp., L. pulchella, M. tectigera and P. maura.

  15. Fungal flora of the digestive tract of Triatoma infestans (Hemiptera: Reduviidae from Argentina Flora fúngica de tractos digestivos en Triatoma infestans (Hemiptera: Reduviidae en Argentina

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    G. A. Marti

    Full Text Available A survey of the fungal microbiota of the digestive tract of Triatoma infestans (Klug (Hemiptera: Reduviidae adults was carried out. Insects captured in the field from different provinces in Argentina, as well as individuals reared in artificial colonies, were used for dissection. Axenic cultures of the fungal species were identified and were deposited with mycological collections at La Plata , Argentina. A total of 33 fungal species, with the exception of three that were mycelia sterilia, belonging to 11 genera were identified. Thirty two species belonged to Ascomycota (Eurotiomycetes and Sordariomycetes and one to Zygomycota (Zygomycetes. The genera with the greatest number of species were Penicillium (15, Aspergillus (5, and Cladosporium (2. Among the isolated fungi, some of the species were entomopathogenic or pathogens of humans and other animals.En el presente estudio se realizó un relevamiento de la flora fúngica microbiana en tractos digestivos de adultos de Triatoma infestans (Klug (Hemiptera: Reduviidae. Se disecaron insectos capturados del campo en diferentes provincias Argentinas, así como también se utilizaron individuos de una colonia artificial. Fueron realizados cultivos axénicos de las especies fúngicas aisladas, los que fueron identificados y luego depositados en las colecciones de hongos entomopatógenos del CEPAVE La Plata , Argentina. Fueron identificadas 33 especies fúngicas perteneciente a 11 géneros. Treinta y dos especies pertenecen a Ascomycota (Eurotiomycetes y Sordariomycetes y una a Zygomycota (Zygomycetes. Los géneros con mayor número de especies fueron Penicillium (15, Aspergillus (5, y Cladosporium (2. Entre los aislamientos fúngicos, algunas de las especies encontradas son entomopatogénicas o patógenas de humanos y otros animales.

  16. Primeiro registro da cochonilha Pendularia paraguariensis Granara de Willink, 1999 (Hemiptera: Coccidae no Brasil First record of Pendularia paraguariensis Granara de Willink, 1999 (Hemiptera: Coccidae in Brazil

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    Adriano Luiz Kussler

    2004-08-01

    Full Text Available Pendularia paraguariensis Granara de WillinK, 1999 (Hemiptera: Coccidae foi registrada atacando plantas de erva-mate, pela primeira vez no Brasil, no município de Chapecó (27°05’47"S, 52°37’06"W,SC de setembro de 2002 a janeiro de 2003, mas outros 32 ervais não apresentaram a ocorrência desse inseto. Esta cochonilha pode causar danos como a degeneração dos ramos primários devido a constante sucção de seiva. O contorno do corpo é oval arredondado (2,68mm x 2,60mm com antenas curtas e robustas com sete segmentos. Esta espécie é caracterizada pela presença e distribuição de poros preoperculares e setas hipopigiais.The presence of Pendularia paraguariensis Granara de WillinK, 1999 (Hemiptera: Coccidae was reported for the first time in Brazil in the town of Chapecó, Santa Catarina State (27°05’47"S, 52°37’06"W, attacking Paraguay tea plants (Ilex paraguariensis, from September 2002 to January 2003. Other 32 Paraguay tea plantations were inspected and there was not occurrence of this insect. This cochineal can cause degeneration of the branches where they are due to the constant suction of the sap. The contour of the body is rounded oval (2.68mm x 2.60mm and it has short and robust antennas with seven segments. This species is characterized by the presence and distribution of preoperculary pores and hypopygial arrows.

  17. Surfactant protein (SP)-A and SP-D as antimicrobial and immunotherapeutic agents.

    Science.gov (United States)

    Awasthi, Shanjana

    2010-06-01

    Surfactant protein (SP)-A and SP-D belong to the "Soluble C-type Lectin" family of proteins and are collectively known as "Collectins". Based on their ability to recognize pathogens and to regulate the host defense, SP-A and SP-D have been recently categorized as "Secretory Pathogen Recognition Receptors". SP-A and SP-D were first identified in the lung; the expression of SP-A and SP-D has also been observed at other mucosal surfaces, such as lacrimal glands, gastrointestinal mucosa, genitourinary epithelium and periodontal surfaces. Since the role of these proteins is not fully elucidated at other mucosal surfaces, the focus of this article is on lung-SP-A and SP-D. It has become clear from research studies performed over a number of years that SP-A and SP-D are critical for the maintenance of lung homeostasis and the regulation of host defense and inflammation. However, none of the surfactant preparations available for clinical use have SP-A or SP-D. A review is presented here on SP-A- and SP-D-deficiencies in lung diseases, the importance of the administration of SP-A and SP-D, and recent patents and research directions that may lead to the design of novel SP-A- or SP-D-based therapeutics and surfactants.

  18. Xestocephalus van Duzee: descrições de seis espécies novas (Hemiptera, Auchenorrhyncha, Cicadellidae, Xestocephalinae Xestocephalus van Duzee: descriptions of six new species (Hemiptera, Auchenorrhyncha, Cicadellidae, Xestocephalinae

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    Adenomar Neves de Carvalho

    2001-09-01

    Full Text Available Six new species of Xestocephalus Van Duzee, 1892 are described: X. crocatus sp. n., X. punctulatus sp. n., X. concolor sp. n., X. coloreus sp. n., X. tripartitus sp. n, and X. stellatus sp. n., the former two from Rondônia and others from Paraná, Brasil.

  19. Revisão das espécies de Ceroplastinae Atkinson (Hemiptera, Coccoidea, Coccidae do Estado de São Paulo, Brasil Revision of the species of Ceroplastinae Atkinson (Hemiptera, Coccoidea, Coccidae from the state of São Paulo, Brazil

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    Ana Lúcia Benfatti Gonzalez Peronti

    2008-01-01

    Full Text Available As espécies de Ceroplastinae (Hemiptera, Coccoidea, Coccidae que ocorrem no Estado de São Paulo são revisadas. São estudadas 36 espécies de Ceroplastinae, das quais 9 são sinonimizadas, 8 espécies novas são descritas e 19 são redescritas. Ceroplastes campinensis Hempel, 1901, C. bicolor Hempel, 1901, C. excaericae Hempel, 1912 e C. farmairii (Signoret, 1866, mencionadas para o Estado de São Paulo, não foram examinadas, devido a não localização do material tipo ou de outros exemplares. Nossos estudos indicaram que C. albolineatus Cockerell, 1894 e C. confluens Cockerell & Tinsley, 1898 foram erroneamente citadas por Hempel, 1900 para o estado de São Paulo. Coccus stellifer Westwood, 1871, atualmente Vinsonia stellifera (Westwood, 1871, é transferida para gênero Ceroplastes como Ceroplastes stellifer (Westwood, 1871 n. comb. C. flosculoides Matile-Ferrero, 1993 é registrada pela primeira vez para o país e C. cassiae (Chavannes, 1848, C. deodorensis Hempel, 1937, C. formosus Hempel, 1900 e C. quadratus Green, 1935 são registradas pela primeira vez no Estado de São Paulo. Ceroplastinae é agora representada por 31 espécies no Estado de São Paulo, todas incluídas no gênero Ceroplastes. Ilustrações e uma chave para espécies são incluídas. Novos sinônimos: C. formicarius Hempel = Ceroplastes communis Hempel, 1900 n. sin.; C. janeirensis Gray, 1828 = Ceroplastes psidii (Chavannes, 1848 n. sin. = C. simplex Hempel, 1900 n. sin.; C. cirripediformis Comstock, 1881 = C. cultus Hempel, 1900 n. sin. = C. cuneatus Hempel, 1900 n. sin. = C. rarus Hempel, 1900 n. sin e C. rotundus Hempel, 1900 n. sin.; C. lucidus Hempel, 1900 = C. novaesi Hempel, 1900 n. sin.; C. grandis Hempel, 1900 = C. rhizophorae Hempel, 1918 n. sin. Novas espécies descritas: C. acutus sp. nov.; C. bragai sp. nov.; C. coronatus sp. nov; C. glomeratus sp. nov; C. jordanensis sp. nov.; C. minimus sp. nov.; C. solanaceus sp. nov.; C. willinkae sp. nov. Esp

  20. Generalized λ-deformations of AdSp × Sp

    Science.gov (United States)

    Chervonyi, Yuri; Lunin, Oleg

    2016-12-01

    We study analytical properties of the generalized λ-deformation, which modifies string theories while preserving integrability, and construct the explicit backgrounds corresponding to AdSp ×Sp, including the Ramond-Ramond fluxes. For an arbitrary coset, we find the general form of the R-matrix underlying the deformation, and prove that the dilaton is not modified by the deformation, while the frames are multiplied by a constant matrix. Our explicit solutions describe families of integrable string theories depending on several continuous parameters.

  1. Population Dynamics of Empoasca fabae (Hemiptera: Cicadellidae) in Central Iowa Alfalfa Fields.

    Science.gov (United States)

    Weiser Erlandson, L A; Obrycki, J J

    2015-01-01

    Adults and nymphs of Empoasca fabae Harris (Hemiptera: Cicadellidae) and adults of predatory species in the families Coccinellidae, Anthocoridae, Nabidae, Chrysopidae, and Hemerobiidae were sampled in Iowa alfalfa fields from June to September in 1999 and 2000. The relationship between each predatory taxa and E. fabae was examined using regression analysis. In 2000, all predators were found to be positively correlated with the presence of E. fabae during all periods sampled and most likely contributed to mortality. Orius insidiosus (Say) (Hemiptera: Anthoridae) was the most numerous insect predatory species; population numbers ranged from 0 to 1 and 0.1 to 3.7 adults per 0.25 m(2) in 1999 and 2000, respectively. Partial life tables were constructed for E. fabae nymphs for two alfalfa-growing periods. Nymphs were grouped into three age intervals: first and second, third and fourth, and fifth instars. For the first alfalfa growing period examined, E. fabae nymphal mortality was 70% in 1999 and 49% in 2000. During the last growing period of each season (August-September), total nymphal mortality was relatively low (<25%). Adult E. fabae density ranged from 5.4 to 25.6 and 1.4-9.2 per 0.25 m(2) in 1999 and 2000, respectively. E. fabae population peaks were similar for each age interval in all growing periods. This study provides further information on the population dynamics of E. fabae and its relationship with select predatory species in Iowa alfalfa fields.

  2. Preparation of Mealybugs (Hemiptera: Pseudococcidae) for Genetic Characterization and Morphological Examination.

    Science.gov (United States)

    Bahder, B W; Bollinger, M L; Sudarshana, M R; Zalom, F G

    2015-01-01

    Mealybugs (Hemiptera: Pseudococcidae) are economically significant agricultural pests on many different crops. Because of their small size and lack of easily visible characters for identification, determination of their taxonomic status is difficult and requires technical competency to prepare a slide-mounted specimen. The standard mounting technique does not allow for analysis of the genome of the specimen. Conversely, preparatory techniques for genetic analysis of mealybugs cause either loss of the entire individual or physical damage that can make morphology-based identification difficult. This study describes a simple protocol that does not impact physical integrity of the specimen for fixation and microscopic examination yet enables simultaneous DNA extraction for DNA-based identification of four mealybug species. All species prepared yielded high quality slide mounts, identified as Planococcus citri Risso, Pseudococcus viburni Signoret, Rhizoecus kondonis Kuwana, or Rhizoecus californicus Ferris. DNA extracted in this manner had higher purity and yield in the final eluate than in samples extracted using standard methods. DNA extracted was successfully amplified by polymerase chain reaction using primers for the cytochrome oxidase I gene and subsequently sequenced for all specimens. This protocol is likely to be applicable to other Hemiptera taxa that are preserved by slide mounting, allowing for both the preparation of a high-quality voucher specimen for morphological identification and simultaneous analysis of DNA for the same specimen. The methods used are technically less challenging than current standard procedures.

  3. Nuevas asociaciones entre Phasiinae (Diptera: Tachinidae y Pentatomidae (Hemiptera: Heteroptera fitófagos en la pampa ondulada (Argentina y descripción del macho de Dallasimyia bosqi Blanchard

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    Gerardo G. LILJESTHRÖM

    2015-01-01

    Full Text Available Las relaciones entre parasitoides Phasiinae (Diptera: Tachinidae y sus huéspedes Pentatomidae (Hemiptera: Heteroptera son escasamente conocidas en la Argentina. Se presentan nuevas asociaciones hospedador-parasitoide en la pampa ondulada, y se describe por primera vez el macho adulto de Dallasimyia bosqi Blanchard. Se efectuaron muestreos quincenales de hemípteros adultos durante primavera-verano de 2005, 2006 y 2012 en áreas de vegetación espontánea y cultivos de trébol rojo y soja en campos de los partidos de La Plata, Magdalena y Chivilcoy (Buenos Aires. Los hemípteros capturados se criaron bajo condiciones controladas, los que tenían huevos de parasitoides pegados en el corion se mantuvieron hasta su muerte, y aquellos sin evidencia de parasitismo se mantuvieron hasta 25-30 días. Cada huésped muerto fue depositado en un recipiente con tierra húmeda y cubierto con una malla de nylon. Los parasitoides adultos emergidos fueron acondicionados para su identificación. Se registraron cinco nuevas asociaciones: E. meditabunda - Cilindromyia brasiliana y Homogenia sp., D. furcatus -Trichopoda argentinensis y Dallasimyia bosqi, y C. armigera - Trichopoda giacomellii, y la ya conocida N. viridula - Trichopoda giacomellii. Colecciones de referencia se encuentran depositadas en el Museo de La Plata, Argentina (MLP y en el Museu de Zoologia da Universidade de São Paulo, Brasil (MZSP.

  4. Description of the male of Psyllaephagus euphyllurae (Masi (Hymenoptera, Encyrtidae, a parasitoid of the olive psylla, Euphyllura olivina (Costa (Hemiptera, Liviidae, with notes on its reproductive traits and hyperparasitoids

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    S.V. Triapitsyn

    2014-12-01

    Full Text Available A colony of the encyrtid wasp Psyllaephagus euphyllurae (Masi (Hymenoptera, Encyrtidae has been established in the quarantine laboratory at the University of California, Riverside, California, USA as part of a classical biological control program against its invasive host, the olive psylla, Euphyllura olivina (Costa (Hemiptera, Psylloidea, Liviidae, an important pest of olives in some parts of the world. The colony originators were reared from the same host found on abandoned, commercial olives in Catalonia, Spain; additional collections were made in Murcia. The parasitoid reproduces primarily by thelytoky; however, a few occasional males have been found in the field in Spain, but not in colonies reared under quarantine or laboratory conditions. Here, the female of P. euphyllurae is redescribed and its male is described and illustrated for the first time; the only previous mention of male P. euphyllurae was from Tunisia, reared from the same psyllid host but without any details on its morphology. A lectotype is designated for Encyrtus euphyllurae Masi. Information is given on the results of genetic matching between the two sexes of the parasitoid and also on the presence of the bacterial Wolbachia symbiont that apparently is affecting reproduction of this species, including its sex ratio in the field. Two species of hyperparasitoids have also emerged from the parasitized olive psylla nymphs from Catalonia: numerous specimens of Apocharips trapezoidea (Hartig (Hymenoptera, Figitidae and one specimen of a Pachyneuron sp. (Hymenoptera, Pteromalidae.

  5. Un género nuevo de Eriococcidae (Hemiptera: Coccoidea de la Argentina A new genus of Eriococcidae (Hemiptera: Coccoidea from Argentina

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    Patricia González

    2008-12-01

    Full Text Available Se describe un género nuevo, Poliloculus gen. nov. Se brinda una clave de los géneros de Eriococcidae de la Argentina. Se describe e ilustra la hembra adulta de Poliloculus stipae sp. nov., encontrada sobre Stipa sp. en la Patagonia.Poliloculus gen. nov. is described. A key to the genera of Eriococcidae of Argentina is given. The adult female of Poliloculus stipae sp. nov. collected in Stipa sp. in the Patagonia is described and illustrated

  6. Nuevas especies de Chorizococcus de la Argentina y Uruguay (Hemiptera: Pseudococcidae New species of Chorizococcus from Argentina and Uruguay (Hemiptera: Pseudococcidae

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    María C. Granara de Willink

    2003-12-01

    Full Text Available Se describen e ilustran dos especies nuevas: Chorizococcus achalensis sp. nov. de la Argentina y Chorizococcus uruguayensis sp. nov., de Uruguay. Se incluye la clave de Chorizococcus McKenzie para la región Neotropical y se citan nuevos huéspedes de Chorizococcus rostellum (Lobdell.Descriptions and illustrations of Chorizococcus achalensis sp. nov. from Argentina and Chorizococcus uruguayensis sp. nov. from Uruguay, are given. Also Chorizococcus Mc Kenzie neotropical key and new hosts plants of Chorizococcus rostellum (Lobdell are included.

  7. Occurrence of Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Aleyrodidae at Rio de Janeiro State = Ocorrência de Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Aleyrodidae no Estado do Rio de Janeiro.

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    Márcio Coutinho de Almeida

    2014-12-01

    Full Text Available Citrus Blackfly’s, Aleurocanthus woglumi (Hemiptera: Aleyrodidae, is considered by the National Plant Protection Organization/Brazilian Ministry of Agriculture (NPPO/MAPA as a present quarantine pest (List A2, which occurs in 20 Brazilian States. The aim of this study is to report its occurrence in the Rio de Janeiro State, since its first notification in 2010. = A Mosca-Negra-dos-Citros, Aleurocanthus woglumi (Hemiptera: Aleyrodidae, é considerada pela Organização Nacional de Proteção Fitossanitária/Ministério da Agricultura (ONPF/MAPA, como praga quarentenária presente (Lista A2, com registros oficiais de ocorrência em 20 Estados brasileiros. O objetivo deste trabalho foi registrar sua ocorrência no Rio de Janeiro desde a sua primeira notificação, no ano de 2010.

  8. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.

    Science.gov (United States)

    Olofsson, Tobias C; Alsterfjord, Magnus; Nilson, Bo; Butler, Eile; Vásquez, Alejandra

    2014-09-01

    We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckii subgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13N(T) ( = DSM 26257(T) = CCUG 63287(T)), Bin4N(T) ( = DSM 26254(T) = CCUG 63291(T)), Hon2N(T) ( = DSM 26255(T) = CCUG 63289(T)), Hma8N(T) ( = DSM 26256(T) = CCUG 63629(T)), Hma2N(T) ( = DSM 26263(T) = CCUG 63633(T)), Bma5N(T) ( = DSM 26265(T) = CCUG 63301(T)) and Biut2N(T) ( = DSM 26262(T) = CCUG 63631(T)).

  9. [Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil].

    Science.gov (United States)

    Guimarães, Antônio Marcos; Alves, Endrigo Gabellini Leonel; de Rezende, Glycia Ferreira; Rodrigues, Marcelo Costa

    2005-04-01

    Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23) and 69.6 (16/23) respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18). Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool samples showed 58% and 23% of Ancylostoma sp and Toxocara sp eggs infection respectively.

  10. Three new species of the leafhopper genus Tautoneura Anufriev (Hemiptera, Cicadellidae, Typhlocybinae from China

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    Yuehua Song

    2011-02-01

    Full Text Available In the present paper, three new species are added to the genus Tautoneura Anufriev from China, T. baiyunshana sp. n., T. caoi sp. n. and T. yunnanensis sp. n. A key to species recorded from China is provided.

  11. Three new species in the leafhopper genus Angustuma (Hemiptera: Cicadellidae: Evacanthinae) from Thailand.

    Science.gov (United States)

    Wang, Yang; Zhang, Yalin

    2014-01-01

    The genus Angustuma Xing & Li, 2013 of the subfamily Evacanthinae is recorded from Thailand for the first time. Three new species, Angustuma basinigra sp. nov., Angustuma flavinigra sp. nov. and Angustuma flavimacula sp. nov., are described and illustrated. Their diagnostic characteristics are compared with close relatives. A key to all known species of Angustuma is provided. 

  12. Membracidae of Gabon: the genus Hamma Buckton, 1905 (Hemiptera: Auchenorrhyncha) with description of three new species.

    Science.gov (United States)

    Loudit, Sandrine Mariela Bayendi; Durante, Antonio; Susini, Antonio

    2014-07-18

    Specimens of Membracidae belonging to the genus Hamma collected in several surveys conducted between 2009 and 2012 in Gabon were examined. They represent six species, of which three are described as new to science: Hamma boulardi n. sp., Hamma carlini n. sp., Hamma franciscae n. sp.

  13. Three new species of the leafhopper genus Dayus Mahmood from China (Hemiptera, Cicadellidae, Typhlocybinae, Empoascini

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    Xiaofei Yu

    2013-11-01

    Full Text Available Three new species of the Oriental empoascine leafhopper genus Dayus Mahmood are described from China: D. bifurcatus sp. n., D. trifurcatus sp. n. and D. serratus sp. n. A key to distinguish all Chinese species of the genus is provided.

  14. First record of occurrence of mosca-negra-dos-citrus, Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Sternorrhyncha: Aleyrodidae) in Roraima, Brazil = Primeiro registro da ocorrência de mosca-negra-dos-citros, Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Aleyrodidae) em Roraima

    OpenAIRE

    Francisco Clemilto da Silva Maciel; Marcos Wanderley da Silva; Anderson Gonçalves da Silva; Ruy Guilherme Correia; Paulo Roberto Silva Farias; Antonio Cesar Silva Lima

    2011-01-01

    The Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae) is an important pest of citrus. Originated in Asia, this pest was detected in Brazil for the first time in the State of Para, Brazil in 2001. In the culture of citrus fruits the black fly carries direct and indirect damage, impeding the development and production of plants. In addition to restricting trade in local areas from its occurrence to the presence of the plague, since the A. woglumi quarantine pest is considered present (A2) of...

  15. A new species of Leafhopper (Hemiptera: Cicadellidae: Typhlocybinae) injurious to Fructus Evodiae%为害吴茱萸的叶蝉——新种(半翅目,叶蝉科,小叶蝉亚科)

    Institute of Scientific and Technical Information of China (English)

    于晓飞; 杨茂发; 孟泽洪

    2012-01-01

    记述采自贵州余庆中药材基地昊茱萸Evodia rutaecarpa( Juss.)Benth.上的小绿叶蝉属Empoasca Walsh,1862松村叶蝉亚属Empoasca(Matsumurasca)Anufriev,1973(半翅目,叶蝉科,小叶蝉亚科)1新种,即吴茱萸小绿叶蝉Empoasca(Matsumurasca)rutaecarpa sp.nov..新种与该亚属其他种区别在于:新种的肛突较狭,直,其末端变尖;阳茎端干腹基部具1腹向突起,其亚端部具1对腹向突起,端干背基部上方具1对小齿突.提供了新种的鉴别特征图.模式标本保存于贵州大学昆虫研究所.%A new species of leafhopper genus Emoposca Walsh, 1862 (Hemiptera, Cicadellidae, Typhlocybinae) injurious to fructus evodiae, Empoasca (Matsumurasca) rutaecarpa sp. nov. , collected from Yuqing County, Guizhou Province, China, was described and illustrated in the present work. The new species is distinguished from the other species of the subgenus Matsumurasca in the following characters: anal tube process straight, long and narrow; aedeagus shaft with a ventral process basally, a paired ventral processes subapical-ly and a paired small dorsal processes basally. The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University.

  16. Bioactivity of the latex from Parahancornia amapa (Apocynaceae on the development of Rhodnius nasutus (Hemiptera, Reduviidae, Triatominae under laboratory conditions

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    Marcio B. P. Lopes

    2014-12-01

    Full Text Available Bioactivity of the latex from Parahancornia amapa (Apocynaceae on the development of Rhodnius nasutus (Hemiptera, Reduviidae, Triatominae under laboratory conditions. The aim of this study was to verify the effects of the methanolic fraction of the latex from Parahancornia amapa (Apocynaceae (PALAM on individuals of the species Rhodnius nasutus Stål (Hemiptera, Triatominae. Many of the insects treated with the substance presented deformities and these may interfere in the feeding and possibly hinder the reproductive capacity. They also presented significant mortality during the molt when compared to the control group, noting a gradual increase in mortality. The treated insects also presented delayed nymphal development (5th instar and higher adult longevity.

  17. The cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae as a new insect pest on tomato plants in Egypt

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    Ibrahim Samah Sayed

    2015-01-01

    Full Text Available Recently, the mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae was recorded as a new pest on tomato plants (Lycopersicon esculentum Mill growing in Egypt. The mealybugs specimens were collected from tomato plants in the Qalyoubia governorate during summer season of 2014. The mealybug was identified as P. solenopsis based on the morphological characters and taxonomic key of this species. This study represents the first record of P. solenopsis as a new insect pest attacking tomato plants in Egypt

  18. Coccophagus scutellaris (Hymenoptera: Aphelinidae): A Highly Effective Biological Control Agent of Soft Scale Insects (Hemiptera: Coccidae) in Egypt

    OpenAIRE

    Shaaban Abd-Rabou

    2011-01-01

    About 953000 individuals of the cosmopolitan parasitoid, Coccophagus scutellaris (Dalman) (Hymenoptera: Aphelinidae), were released and evaluated during 2009-2010 for the control of the following soft scale insects (Hemiptera: Coccidae) infesting the following economic crops in Egypt: Ceroplastes rusci on citrus in Beni Seuf, Ceroplastes floridensis Comstock on citrus in Gharbiya, Coccus hesperidum L. on guava in Giza, Pulvinaria floccifera (Westwood) on mango in Sharqiya, Pulvinaria psidii...

  19. A systematic study of Peliococcus Borchsenius (Hemiptera: Coccoidea: Pseudococcidae), with descriptions of a new Palaearctic genus and four new species from Turkey.

    Science.gov (United States)

    Kaydan, Mehmet Bora

    2015-02-20

    Within the Phenacoccinae (Hemiptera: Coccoidea: Pseudococcidae), there are four nominal genera that include species with clusters or groups of multilocular disc pores and/or oral collar tubular ducts of more than one size, namely Peliococcus Borchsenius (= Spinococcus Borchsenius and Eupeliococcus Săvescu), Peliococcopsis Borchsenius, Erimococcus Ezzat, and Seyneria Goux. This study analysed molecular data (from the mitochondrial gene cytochrome oxidase I, COI, and a fragment of the D2 and D3 regions of the large subunit ribosomal DNA gene, 28S) from Palaearctic species of Peliococcus, Peliococcopsis and a new genus, Pelionella Kaydan to demonstrate: (i) that Peliococcus and several morphologically similar genera do not form a monophyletic group; (ii) Peliococcus, as currently understood, is not monophyletic, and (iii) the structure of the multilocular disc pores is important for recognising the systematic position of these genera. The morphological data were fitted to the molecular tree to allow reassessment of the generic classification and to produce morphological diagnoses of the genera. Five species have been moved to the genus Erimococcus Ezzat: Peliococcus kimmericus Kiritchenko, P. montanus Bazarov & Babaeva, P. multitubulatus (Danzig), P. salviae Hadzibejli and P. talhouki Matille-Ferrero, as E. kimmericus (Kiritchenko) comb. nov., E. montanus (Bazarov & Babaeva) comb. nov., E. multitubulatus (Danzig) comb. nov., E. salviae (Hadzibejli) comb.nov. and E. talhouki (Matille-Ferrero) comb. nov. In addition, a new species of Erimococcus is described, namely Erimococcus ozani Kaydan sp. nov. Also Peliococcus ilamicus Moghaddam syn. nov. is recognised as a junior synonym of Phenacoccus kimmericus Kiritchenko (previously in Peliococcus, now in Erimococcus). A new genus, Pelionella gen. nov. is introduced to take nine species, namely Peliococcus balteatus Green, P. cycliger Leonardi, P. manifectus Borchsenius, P. stellarocheae Goux, P. glandulifer Borchsenius, P

  20. Comparative biology of the two sister species of Triatominae (Hemiptera: Reduviidae Biologia comparativa de duas espécies irmãs de Triatominae (Hemiptera: Reduviidae

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    Ana Laura Carbajal de la Fuente

    2010-02-01

    Full Text Available INTRODUCTION: Triatoma pseudomaculata and T. wygodzinskyi (Hemiptera: Reduviidae: Triatominae are two Brazilian vectors of Chagas disease. The first is an arboricolous species in sylvatic environment and considered a vector of T. cruzi in peridomestic structures; the second, a rupicolous species in the wild environment of no epidemiological importance. In order to test the assumption that sister species share biological traits, comparative studies of their development cycle and blood ingestion were conducted. METHODS: Eggs laid by five field females of each species were randomly selected. The nymphs were observed daily and fed on mice weekly. The time required to pass through the different stages to adulthood was recorded in days. The triatomines were weighed individually before and after feeding. The mortality rate according to each nymphal stage was calculated. RESULTS AND CONCLUSIONS: Analysis of the results shows that they display only minor biological differences even though they exhibit a distinct ecology. This suggests that the biological traits are important criteria to determine the relationship between species.INTRODUÇÃO: Triatoma pseudomaculata e T. wygodzinskyi (Hemiptera: Reduviidae: Triatominae são dois vetores Brasileiros da doença de Chagas. A primeira é uma espécie arborícola em ambiente silvestre e considerada vetor do T. cruzi em estruturas peridomesticas. A segunda é rupícola em ambientes silvestres e sem importância epidemiológica. Com o objetivo de testar a hipótese que espécies irmãs compartilham características semelhantes, realizamos um estudo comparativo do ciclo biológico e ingesta alimentar. MÉTODOS: Ovos pertencentes a cinco fêmeas de cada espécie provenientes do campo foram selecionados aleatoriamente. As ninfas foram observadas diariamente e alimentadas com camundongos semanalmente. O tempo requerido para passar até o estágio adulto foi registrado em dias. Os triatomíneos foram pesados

  1. Desenvolvimento de Dysmicoccus brevipes (Cockerell (Hemiptera:Pseudococcidae em duas cultivares de abacaxi Development of the pineapple mealybug Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae on two pineapple cultivars

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    Lenira Viana Costa Santa-Cecília

    2004-10-01

    Full Text Available A cochonilha Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae é uma das principais pragas em cultivos de abacaxi e os estudos biológicos desse inseto em diferentes cultivares são uma necessidade. Objetivou-se com este trabalho avaliar o desenvolvimento dessa cochonilha em duas cultivares de abacaxi, Pérola e Cayenne. O experimento foi conduzido em câmara climatizada a 25 ± 1ºC, 70 ± 10% UR e fotofase de 12 horas. As plantas de abacaxi foram reproduzidas in vitro, transplantadas para vasos (250 mL e aclimatadas em casa-de-vegetação. Ninfas com até um dia de vida foram confinadas em gaiolas de PVC (1 cm de diâmetro, e fixadas em folhas de ambas as cultivares. Não foram detectadas diferenças no desenvolvimento de D. brevipes nos dois substratos alimentares utilizados. O desenvolvimento ninfal de fêmeas e machos de D. brevipes foi 39,9 e 32,0 dias na cv. Pérola e 38,5 e 32,4 dias na cv. Cayenne, respectivamente. A sobrevivência da fase ninfal foi 32,3 e 40,5%; a razão sexual, 0,39 e 0,33; e a longevidade de fêmeas, 20,3 e 26,1 dias nas cvs. Pérola e Cayenne, respectivamente.The pineapple mealybug Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae is the main pest on pineapple crops and biological studies of this insect are necessary on different cultivars. The objective of this work was to evaluate the development time of this mealybug on the pineapple cultivars Pérola and Cayenne. The experiment was performed in climated chambers at 25 ± 1ºC, 70 ± 10% RH and 12 h photophase. Pinneapple plants were reproduced in vitro, transplanted to pots (250 mL and kept in greenhouse. One-day-old nymphs were kept inside a clipcage (1 cm diameter fixed on the plants. No differences in development of D. brevipes among the cultivars were found. Development times of females and males, respectively, were 39.9 and 32 days on cv. Pérola and 38.5 and 32.4 days on cv. Cayenne. Nymphs survivals were 32.3 and 40.5%, sexual rates

  2. An Sp1/Sp3 binding polymorphism confers methylation protection.

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    Yanis A Boumber

    2008-08-01

    Full Text Available Hundreds of genes show aberrant DNA hypermethylation in cancer, yet little is known about the causes of this hypermethylation. We identified RIL as a frequent methylation target in cancer. In search for factors that influence RIL hypermethylation, we found a 12-bp polymorphic sequence around its transcription start site that creates a long allele. Pyrosequencing of homozygous tumors revealed a 2.1-fold higher methylation for the short alleles (P<0.001. Bisulfite sequencing of cancers heterozygous for RIL showed that the short alleles are 3.1-fold more methylated than the long (P<0.001. The comparison of expression levels between unmethylated long and short EBV-transformed cell lines showed no difference in expression in vivo. Electrophorectic mobility shift assay showed that the inserted region of the long allele binds Sp1 and Sp3 transcription factors, a binding that is absent in the short allele. Transient transfection of RIL allele-specific transgenes showed no effects of the additional Sp1 site on transcription early on. However, stable transfection of methylation-seeded constructs showed gradually decreasing transcription levels from the short allele with eventual spreading of de novo methylation. In contrast, the long allele showed stable levels of expression over time as measured by luciferase and approximately 2-3-fold lower levels of methylation by bisulfite sequencing (P<0.001, suggesting that the polymorphic Sp1 site protects against time-dependent silencing. Our finding demonstrates that, in some genes, hypermethylation in cancer is dictated by protein-DNA interactions at the promoters and provides a novel mechanism by which genetic polymorphisms can influence an epigenetic state.

  3. Five new species of Ishiharella Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae) with a key to worldwide species of the genus.

    Science.gov (United States)

    Yu, Xiaofei; Yang, Maofa; Dietrich, Christopher H

    2015-01-01

    Detailed morphological descriptions and illustrations of five new species of the empoascine leafhopper genus Ishiharella Dworakowska (Typhlocybinae: Emposascini): I. dentidensa Yu & Yang, sp. nov., I. falcata Yu, Yang & Dietrich, sp. nov., I. hirsuta Yu, Yang & Dietrich, sp. nov., I. inflata Yu & Yang, sp. nov. and I. multiprotrusa Yu, Yang & Dietrich, sp. nov. are provided and a key to species of the genus is given.

  4. Advances in the use of the RNA interference technique in Hemiptera

    Institute of Scientific and Technical Information of China (English)

    Jie Li; Xiao-Ping Wang; Man-Qun Wang; Wei-Hua Ma; Hong-Xia Hua

    2013-01-01

    RNA interference(RNAi)suppresses the expression of target genes by posttranscriptional regulation.Because double-stranded RNA(dsRNA)mediated gene silencing is a conserved mechanism in many eukaryotes,RNAi has become a valuable tool for unveiling gene function in many model insects.Recent research has also shown that RNAi can also be effective in the downregulation of target genes in Hemiptera.In this review,we discuss the use of the RNAi technique in gene functional analysis in hemipterans,highlighting the methods of dsRNA uptake by these insects and discuss the knock-down efficiency of these techniques.Although the RNAi technique has disadvantages,our primary goal here is to determine whether it can be exploited further in the discovery of new gene functions,and as a pest control strategy,in some important Hemipteran pests.

  5. Phylogenetic signals from Nepomorpha (Insecta: Hemiptera: Heteroptera) mouthparts: stylets bundle, sense organs, and labial segments.

    Science.gov (United States)

    Brożek, Jolanta

    2014-01-01

    The present study is a cladistic analysis of morphological characters focusing on the file of the mandible, the apices of the maxillae, the rupturing device on the maxillae, the internal structures of the mouthparts, and the external morphology of the labial segments as well as the distribution of labial sensilla in true water bugs (Hemiptera: Heteroptera, infraorder Nepomorpha). The study is based on data referring to sixty-two species representing all nepomorphan families (Heteroptera), together with one outgroup species representing the infraorders Gerromorpha (Mesoveliidae). The morphological data matrix consists of forty-eight characters. The present hypothesis supports the monophyly of the Nepomorpha and the monophyly of all families. The new modification in the systematic classification has been proposed: ((Nepidae + Belostomatidae), (Diaprepocoridae + Corixidae + Micronectidae), (Ochteridae + Gelastocoridae), Aphelocheiridae, Potamocoridae, Naucoridae, Notonectidae, and (Pleidae + Helotrephidae)).

  6. Phylogenetic Signals from Nepomorpha (Insecta: Hemiptera: Heteroptera Mouthparts: Stylets Bundle, Sense Organs, and Labial Segments

    Directory of Open Access Journals (Sweden)

    Jolanta Brożek

    2014-01-01

    Full Text Available The present study is a cladistic analysis of morphological characters focusing on the file of the mandible, the apices of the maxillae, the rupturing device on the maxillae, the internal structures of the mouthparts, and the external morphology of the labial segments as well as the distribution of labial sensilla in true water bugs (Hemiptera: Heteroptera, infraorder Nepomorpha. The study is based on data referring to sixty-two species representing all nepomorphan families (Heteroptera, together with one outgroup species representing the infraorders Gerromorpha (Mesoveliidae. The morphological data matrix consists of forty-eight characters. The present hypothesis supports the monophyly of the Nepomorpha and the monophyly of all families. The new modification in the systematic classification has been proposed: ((Nepidae + Belostomatidae, (Diaprepocoridae + Corixidae + Micronectidae, (Ochteridae + Gelastocoridae, Aphelocheiridae, Potamocoridae, Naucoridae, Notonectidae, and (Pleidae + Helotrephidae.

  7. Survival and fecundity of two strains of Cimex lectularius (Hemiptera: Heteroptera).

    Science.gov (United States)

    Barbarin, Alexis M; Barbarin, Alexis M; Barbu, Corentin M; Gebhardtsbauer, Ron; Rajotte, Edwin G

    2014-09-01

    Knowledge of development of the bed bug Cimex lectularius L. (Hemiptera: Heteroptera) under specific environmental conditions could lead to improved management techniques. Developmental rates, age-, and stage-specific life tables were compared for a laboratory strain and a field strain of bed bugs reared on human blood. Both strains were then crossed reciprocally to produce four F1 generations and subsequent age- and stage-specific life tables were constructed. No significant differences were found in the overall survival of the parental strains, but significant differences were found in development rate within various instars based on strain. Parallel results were derived from the F1 generation hybrids. Stable age distribution calculations predict that 80% of bedbugs within exponentially growing populations will be immature.

  8. Leaf Damaged by Nymph of Cardiaspina albitextura and Cardiaspina retator (Hemiptera: Psyllidae

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    NISMAH

    2008-06-01

    Full Text Available Cardispina (Hemiptera: Pshyllidae is one of the most important genera of the Australian Psyllidae, owing to the spectacular damage to Eucalyptus spp. The increase of C. albitextura and C. retator populations in high numbers caused severe damage on leaves of Eucalyptus camaldulensis. Both species have similarities, i.e. they occur on the same host plant and prefer mature leaves of E. camaldulensis for oviposition and feeding sites. They might thus be more likely to show competition than the less closely related taxa. The purpose of the study was to examine degree of the resource use by measuring the area of leaves damaged by the nymphs of C. albitextura and C. retator. The results indicated that the nymph of C. retator caused damage twice more damages than that of C. albitextura. The leaves area damaged by the females of both species was greater than that by the males.

  9. An annotated update of the scale insect checklist of Hungary (Hemiptera, Coccoidea

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    Ferenc Kozár

    2013-06-01

    Full Text Available The number of scale insect species (Hemiptera: Coccoidea known from Hungary has increased in the last 10 years by 39 (16.6 %, to a total of 274 species belonging to 112 genera in10 families. The family Pseudococcidae is the most species rich, with 101 species in 34 genera; Diaspididae contains 59 species in 27 genera; Coccidae contains 54 species in 27 genera; and the Eriococcidae contains 33 species in 8 genera. The other 6 coccoid families each contain only a few species: Asterolecaniidae (7 species in 3 genera; Ortheziidae (7 species in 4 genera; Margarodidae sensu lato (5 species in 5 genera; Cryptococcidae (3 species in 2 genera; Kermesidae (4 species in 1genus; and Cerococcidae (1 species. Of the species in the check list, 224 were found in outdoor conditions, while 50 species occurred only in indoor conditions. This paper contains 22 species recorded for the first time in the Hungarian fauna.

  10. Are Phenacoccus solani Ferris and P. defectus Ferris (Hemiptera: Pseudococcidae) distinct species?

    Science.gov (United States)

    Chatzidimitriou, Evangelia; Simonato, Mauro; Watson, Gillian W; Martinez-Sañudo, Isabel; Tanaka, Hirotaka; Zhao, Jing; Pellizzari, Giuseppina

    2016-03-24

    Among the Nearctic species of Phenacoccus (Hemiptera: Pseudococcidae), Phenacoccus solani Ferris and P. defectus Ferris are morphologically similar and it can be difficult to separate them on the basis of microscopic morphological characters of the adult female alone. In order to resolve their identity, a canonical variates morphological analysis of 199 specimens from different geographical origins and host plants and a molecular analysis of the COI and 28S genes were performed. The morphological analysis supported synonymy of the two species, as although the type specimens of the "species" are widely separated from each other in the canonical variates plot, they are all part of a continuous range of variation. The molecular analysis showed that P. solani and P. defectus are grouped in the same clade. On the basis of the morphological and molecular analyses, P. defectus is synonymized under the senior name P. solani, syn. n.

  11. The structure of integument and wax glands of Phenacoccus fraxinus (Hemiptera: Coccoidea: Pseudococcidae).

    Science.gov (United States)

    Zhang, Yanfeng; Xie, Yingping; Xue, Jiaoliang; Fu, Xiaohong; Liu, Weimin

    2012-06-01

    Using scanning electron microscopy and optical microscopy, we studied the structure of the integument and wax glands of the mealybug, Phenacoccus fraxinus Tang (Hemiptera: Coccoidea: Pseudococcidae). We observed the ultrastructure of four wax pores including trilocular, quinquelocular, and multilocular pores as well as tubular ducts, recording characteristics of their structure, size and distribution. We found that that the integument of the mealybug consists of three main layers-the procuticle, epidermis and basement membrane-and four sub-layers of the procuticle-the epicuticle, exocuticle, endocuticle and formation zone. The wax-secreting gland cells were closely arranged in epidermis. All of them were complex and composed of one central cell and two or more lateral cells. These complex cells possess a large common reservoir for collection and storage. Synthesized by the glandular cells, the wax is excreted outside integument through canals.

  12. Chirality and bioactivity of the sex pheromone of Madeira mealybug (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Ho, Hsiao-Yung; Ko, Chi-Hung; Cheng, Chao-Chih; Su, Yu-Ting; Pola, Someshwar

    2011-06-01

    Two compounds (trans-1R,3R-chrysanthemyl R-2-methylbutanoate and R-lavandulyl R-2-methylbutanoate) identified from aeration extracts of virgin female Madeira mealybug, Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae), were synthesized and tested in field bioassays in northern Taiwan over a 1-mo period. In total, 1,492 male P. madeirensis were captured in sticky traps. Our results showed that 1 microg of synthetic trans-1R,3R-chrysanthemyl R-2-methylbutanoate released from a plastic tube dispenser was attractive to the mealybugs. Different stereoisomers of chrysanthemyl 2-methylbutanoate also were tested. The insect-produced stereoisomer was the most attractive of all the isomers tested, and the stereochemistry of the acid moiety proved to be more critical than that of the alcohol moiety. The minor component found in extracts, R-lavandulyl R-2-methylbutanoate, alone was not attractive to male Madeira mealybugs nor did it act synergistically or additively with the main component.

  13. Genome sequence of "Candidatus Walczuchella monophlebidarum" the flavobacterial endosymbiont of Llaveia axin axin (Hemiptera: Coccoidea: Monophlebidae).

    Science.gov (United States)

    Rosas-Pérez, Tania; Rosenblueth, Mónica; Rincón-Rosales, Reiner; Mora, Jaime; Martínez-Romero, Esperanza

    2014-03-01

    Scale insects (Hemiptera: Coccoidae) constitute a very diverse group of sap-feeding insects with a large diversity of symbiotic associations with bacteria. Here, we present the complete genome sequence, metabolic reconstruction, and comparative genomics of the flavobacterial endosymbiont of the giant scale insect Llaveia axin axin. The gene repertoire of its 309,299 bp genome was similar to that of other flavobacterial insect endosymbionts though not syntenic. According to its genetic content, essential amino acid biosynthesis is likely to be the flavobacterial endosymbiont's principal contribution to the symbiotic association with its insect host. We also report the presence of a γ-proteobacterial symbiont that may be involved in waste nitrogen recycling and also has amino acid biosynthetic capabilities that may provide metabolic precursors to the flavobacterial endosymbiont. We propose "Candidatus Walczuchella monophlebidarum" as the name of the flavobacterial endosymbiont of insects from the Monophlebidae family.

  14. An annotated checklist of scale insects (Hemiptera: Coccoidea) of Saint Lucia, Lesser Antilles .

    Science.gov (United States)

    Malumphy, Chris

    2014-07-31

    An annotated list of 83 scale insect species (Hemiptera: Sterorrhyncha: Coccoidea) recorded from Saint Lucia is presented, based on data gathered from UK quarantine interceptions, samples collected in an urban coastal habitat in the North West of the Island in 2013, and published records. Thirty-three species (40%) are recorded for the first time for the country, including Dysmicoccus joannesiae (Costa Lima), a South American mealybug, and Poliaspoides formosana (Takahashi), an Asian armoured scale insect pest of bamboo, which are new for the Caribbean region. The economic, environmental and social impacts caused by introduced exotic species of scale insect are discussed. Two predatory midges Diadiplosis ?coccidivora (Felt) and Diadiplosis multifila (Felt) (Diptera: Cecidomyiidae) are recorded for the first time from Saint Lucia. The latter species was observed causing 90% mortality of a large infestation of passion vine mealybug Planococcus minor (Maskell) on soursop fruit. 

  15. An annotated update of the scale insect checklist of Hungary (Hemiptera, Coccoidea).

    Science.gov (United States)

    Kozár, Ferenc; Benedicty, Zsuzsanna Konczné; Fetykó, Kinga; Kiss, Balázs; Szita, Eva

    2013-01-01

    The number of scale insect species (Hemiptera: Coccoidea) known from Hungary has increased in the last 10 years by 39 (16.6 %), to a total of 274 species belonging to 112 genera in10 families. The family Pseudococcidae is the most species rich, with 101 species in 34 genera; Diaspididae contains 59 species in 27 genera; Coccidae contains 54 species in 27 genera; and the Eriococcidae contains 33 species in 8 genera. The other 6 coccoid families each contain only a few species: Asterolecaniidae (7 species in 3 genera); Ortheziidae (7 species in 4 genera); Margarodidaesensu lato (5 species in 5 genera); Cryptococcidae (3 species in 2 genera); Kermesidae (4 species in 1genus); and Cerococcidae (1 species). Of the species in the check list, 224 were found in outdoor conditions, while 50 species occurred only in indoor conditions. This paper contains 22 species recorded for the first time in the Hungarian fauna.

  16. Wolbachia infection density in populations of the Asian citrus psyllid (Hemiptera: Liviidae).

    Science.gov (United States)

    Hoffmann, M; Coy, M R; Kingdom Gibbard, H N; Pelz-Stelinski, K S

    2014-10-01

    The symbiotic relationships between bacteria of the genus Wolbachia (order Rickettsiales) and their arthropod hosts are diverse and can range from mutualism to parasitism. Whereas effects of Wolbachia on host biology are well investigated, little is known about diversity and abundance of Wolbachia in their natural hosts. The phloem-feeding Asian citrus psyllid, Diaphorina citri (Kuwayama) (Hemiptera: Liviidae), is naturally infected with Wolbachia (wDi). In the current study, we calculated the within-host density of Wolbachia in Florida D. citri populations using quantitative polymerase chain reaction for detection of the Wolbachia outer surface protein gene, wsp. Gene quantities were normalized to the D. citri wingless gene (Wg) to estimate Wolbachia abundance in individual D. citri. Using this method, significant geographic differences in Wolbachia densities were detected among Florida D. citri populations, with higher infection levels occurring in male versus female hosts.

  17. Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands.

    Science.gov (United States)

    Heyrman, Jeroen; Vanparys, Bram; Logan, Niall A; Balcaen, An; Rodríguez-Díaz, Marina; Felske, Andreas; De Vos, Paul

    2004-01-01

    A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA-DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).

  18. On dorsal prothoracic appendages in treehoppers (Hemiptera: Membracidae and the nature of morphological evidence.

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    István Mikó

    Full Text Available A spectacular hypothesis was published recently, which suggested that the "helmet" (a dorsal thoracic sclerite that obscures most of the body of treehoppers (Insecta: Hemiptera: Membracidae is connected to the 1st thoracic segment (T1; prothorax via a jointed articulation and therefore was a true appendage. Furthermore, the "helmet" was interpreted to share multiple characteristics with wings, which in extant pterygote insects are present only on the 2nd (T2 and 3rd (T3 thoracic segments. In this context, the "helmet" could be considered an evolutionary novelty. Although multiple lines of morphological evidence putatively supported the "helmet"-wing homology, the relationship of the "helmet" to other thoracic sclerites and muscles remained unclear. Our observations of exemplar thoraces of 10 hemipteran families reveal multiple misinterpretations relevant to the "helmet"-wing homology hypothesis as originally conceived: 1 the "helmet" actually represents T1 (excluding the fore legs; 2 the "T1 tergum" is actually the anterior dorsal area of T2; 3 the putative articulation between the "helmet" and T1 is actually the articulation between T1 and T2. We conclude that there is no dorsal, articulated appendage on the membracid T1. Although the posterior, flattened, cuticular evagination (PFE of the membracid T1 does share structural and genetic attributes with wings, the PFE is actually widely distributed across Hemiptera. Hence, the presence of this structure in Membracidae is not an evolutionary novelty for this clade. We discuss this new interpretation of the membracid T1 and the challenges of interpreting and representing morphological data more broadly. We acknowledge that the lack of data standards for morphology is a contributing factor to misinterpreted results and offer an example for how one can reduce ambiguity in morphology by referencing anatomical concepts in published ontologies.

  19. On dorsal prothoracic appendages in treehoppers (Hemiptera: Membracidae) and the nature of morphological evidence.

    Science.gov (United States)

    Mikó, István; Friedrich, Frank; Yoder, Matthew J; Hines, Heather M; Deitz, Lewis L; Bertone, Matthew A; Seltmann, Katja C; Wallace, Matthew S; Deans, Andrew R

    2012-01-01

    A spectacular hypothesis was published recently, which suggested that the "helmet" (a dorsal thoracic sclerite that obscures most of the body) of treehoppers (Insecta: Hemiptera: Membracidae) is connected to the 1st thoracic segment (T1; prothorax) via a jointed articulation and therefore was a true appendage. Furthermore, the "helmet" was interpreted to share multiple characteristics with wings, which in extant pterygote insects are present only on the 2nd (T2) and 3rd (T3) thoracic segments. In this context, the "helmet" could be considered an evolutionary novelty. Although multiple lines of morphological evidence putatively supported the "helmet"-wing homology, the relationship of the "helmet" to other thoracic sclerites and muscles remained unclear. Our observations of exemplar thoraces of 10 hemipteran families reveal multiple misinterpretations relevant to the "helmet"-wing homology hypothesis as originally conceived: 1) the "helmet" actually represents T1 (excluding the fore legs); 2) the "T1 tergum" is actually the anterior dorsal area of T2; 3) the putative articulation between the "helmet" and T1 is actually the articulation between T1 and T2. We conclude that there is no dorsal, articulated appendage on the membracid T1. Although the posterior, flattened, cuticular evagination (PFE) of the membracid T1 does share structural and genetic attributes with wings, the PFE is actually widely distributed across Hemiptera. Hence, the presence of this structure in Membracidae is not an evolutionary novelty for this clade. We discuss this new interpretation of the membracid T1 and the challenges of interpreting and representing morphological data more broadly. We acknowledge that the lack of data standards for morphology is a contributing factor to misinterpreted results and offer an example for how one can reduce ambiguity in morphology by referencing anatomical concepts in published ontologies.

  20. Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae); Efeitos da temperatura e do alimento no desenvolvimento de Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Fabio Souto [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Ciencias Ambientais], e-mail: fbio_almeida@yahoo.com.br; Goncalves, Lenicio [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Biologia Animal. Area de Biologia], e-mail: lencygon@globo.com

    2007-10-15

    Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae) is an important pest on Gossypium spp. (cotton tree), Citrus sinensis Osbeck (Rutaceae) and Citrus reticulata Blanco (Rutaceae) crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae). This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 {+-} 1 deg C, 80 {+-} 3% RH and 12h photo phase or in laboratory conditions (23.5 {+-} 2.6 deg C, 73.3 {+-} 9.9 % RH), and in the other two treatments bugs were fed with seeds of cotton variety IAC-22 and kept at 25 or 30 deg C. In all treatments five immature stages were observed. The increase of temperature caused reduction in the developmental time. The temperature of 15 deg C disabled nymphal eclosion and was also lethal to those nymphs ecloded at other temperatures. The lower mortality of nymphs occurred in the temperature of 25 deg C with cotton as food (24.07%). The lower threshold temperature (Tb) occurred for the first instar (11.54 deg C) and the higher for the second instar (15.33 deg C). The females of D. maurus required more degree-days (329.93 degree-days) than males (300.49 degree-days) until adult emergence. (author)

  1. A review of the leafhopper tribe Hyalojassini (Hemiptera: Cicadellidae: Iassinae) with description of new taxa.

    Science.gov (United States)

    Dai, Wu; Dietrich, Christopher H; Zhang, Yalin

    2015-01-01

    The morphologically diverse leafhopper tribe Hyalojassini is reinstated from synonymy under Iassini based on distinctive features of the male genitalia and a key to the Oriental genera is given. The previously monotypic genera Hyalojassus Evans and Coriojassus Evans are revised and redescribed based on study of the type species. The following new genera and species are described from Thailand and China and placed in Hyalojassini: Kanchanaburiassus maculatus gen. nov. & sp. nov. from Kanchanaburi, Thailand; Lamelliassus chaingmaiensis gen. & sp. nov. from Chaingmai, Thailand; Siamiassus constanti gen. & sp. nov. from Loei, Thailand ; Decliviassus gen nov. with D. bipunctatus sp. nov., D. maculatus sp. nov., and D. nudus sp. nov., from Thailand; Trocniassus gen. nov. with T. shaanxiensis and T. henanensis sp. nov.; Hyalojassus elongatus sp. nov. and H. punctulatus sp. nov. from Thailand and H. yunnanensis sp. nov. from China; Coriojassus loeiensis sp. nov. from Thailand, C. yunnanensis sp. nov. and C. zhejiangensis sp. nov. from Yunnan and Zhejiang, China, respectively, the latter representing the first records of the genus from China. Sinojassus Dai et al. 2010 (nec Zhang 1985) is a junior homonym, thus a new replacement name, Siniassus nom. nov., is proposed, the genus is transferred to Hyalojassini, and the following new combinations are made: Siniassus loberus (Dai, Zhang & Zhang, 2010), Siniassus aspinus (Dai, Zhang & Zhang, 2010), Siniassus compressus (Dai, Zhang & Zhang, 2010) and Siniassus webbi (Dai & Dietrich, 2010). Detailed morphological descriptions and illustrations for all new taxa are provided. The following New World genera share the diagnostic morphological features of Hyalojassini and are newly placed in this tribe: Absheta Blocker, Aztrania Blocker, Baldriga Blocker, Bertawolia Blocker, Betawala Blocker, Comanopa Blocker, Daveyoungana Blocker & Webb, Derakandra Blocker, Donleva Blocker, Gargaropsis Fowler, Garlica Blocker, Gehundra Blocker

  2. First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil

    Directory of Open Access Journals (Sweden)

    Sônia M. F. Broglio

    2012-03-01

    Full Text Available First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil. This is the first report of a severe attack of Teleonemia morio (Stål, 1855 (Hemiptera, Heteroptera, Tingidae on Annona squamosa L. (custard apple, causing up to 80% of losses of infested trees. In order to facilitate the identification of this insect pest, the adult female of T. morio is redescribed based on specimens collected in Palmeira dos Índios, Alagoas, Brazil.

  3. SP-A and SP-D in host defense against fungal infections and allergies.

    Science.gov (United States)

    Pandit, Hrishikesh; Madhukaran, Shanmuga P; Nayak, Annapurna; Madan, Taruna

    2012-01-01

    Innate immunity mediated by pattern recognition proteins is relevant in the host defense against fungi. SP-A and SP-D are two such proteins belonging to the class of collagen domain containing C-type lectins, or collectins. They bind to the sugar moieties present on the cell walls of various fungi in a dose dependent manner via their carbohydrate recognition domain (CRD). SP-A and SP-D directly interact with alveolar macrophages, neutrophils, lymphocytes. We review these roles of SP-A and SP-D against various clinically relevant fungal pathogens and fungal allergens. SP-A and SP-D gene deficient mice showed increased susceptibility/ resistance to various fungal infections. Patients of fungal infections and allergies are reported with alterations in the serum or lung lavage levels of SP-A and SP-D. There are studies associating the gene polymorphisms in SP-A and SP-D with alterations in their levels or functions or susceptibility of the host to fungal diseases. In view of the protective role of SP-D in murine models of Aspergillus fumigatus infections and allergies, therapeutic use of SP-D could be explored further.

  4. Additions to the leafhopper subgenus Pediopsoides (Pediopsoides) Matsumura, 1912 (Hemiptera: Cicadellidae: Macropsinae) from Southern China.

    Science.gov (United States)

    Li, Hu; Li, Zi-Zhong

    2016-01-01

    Three new species of the leafhopper subgenus Pediopsoides (Pediopsoides) Matsumura, 1912, P. (P.) alba Li, Dai & Li sp. nov. from Yunnan Province, Southwestern China, P. (P.) amplificata Li, Dai & Li sp. nov. and P. (P.) longiapophysis Li, Dai & Li sp. nov. from Guangdong Province, Southern China, are described and illustrated. A key and an updated checklist with distribution to the Pediopsoides (Pediopsoides) species along with geographical distribution maps of the new species are given.

  5. A new family of aphids (Hemiptera, Sternorrhyncha from the Lower Cretaceous of Baissa, Transbaikalia

    Directory of Open Access Journals (Sweden)

    Agnieszka Homan

    2011-09-01

    Full Text Available The family Rasnitsynaphididae fam. n. has a unique combination of characters: 9-segmented antennae; rhinaria arranged in many transverse rows, surrounding the antennal segments; segment IX narrower than other segments of flagellum, always without rhinaria; cubitus branches separated; ovipositor present; siphuncular pores absent. The new family comprises the genus Rasnitsynaphis gen. n. with three species, R. ennearticulata sp. n., R. coniuncta sp. n., and R. quadrata sp. n., all from the Lower Cretaceous of Transbaikalia.

  6. Tres nuevas especies y nuevos registros de Ladoffa de Panamá (Insecta: Hemiptera: Cicadellidae: Cicadellinae

    Directory of Open Access Journals (Sweden)

    Pedro W. Lozada

    2011-05-01

    Full Text Available Se describen e ilustran tres nuevas especies panameñas del género Ladoffa: woldai Lozada & Freytag sp.nov., lamasi Lozada & Freytag sp.nov. y grandis Lozada & Freytag sp.nov. Asimismo, se dan a conocer nuevos registros para Panamá de las especies variolaria Young, donsana Young, rubriguttata (Walker, elauta Young, dependens Young, uncata Young y trifasciata Cavichioli & Chiamolera.

  7. Potensi Tanaman Ornamental (Aglaonema sp., Dieffenbachia sp., dan Spathiphyllum sp.) dalam Menurunkan Jumlah Mikroba Udara dalam Ruangan Kelas Sekolah Dasar

    OpenAIRE

    Mangunsong, Sisca Nency Teresia

    2016-01-01

    Bioaerosol is dust particles consisting of bacteria and other fungi with spores that are in the room when the temperature and humidity level are adequate. Its presence in the room are generally harmless, but some time causes disease. This research was aims to determine the effect of ornamental plant Aglaonema sp., Dieffenbachia sp., and Spathiphyllum sp. on amount of bacteria and fungi in the class room. Bioaerosol isolation was performed according to the method of air sampling with three rep...

  8. The first Aleyrodidae from the Lowermost Eocene Oise amber (Hemiptera: Sternorrhyncha).

    Science.gov (United States)

    Drohojowska, Jowita; Szwedo, Jacek

    2013-01-01

    The first records are provided of the family Aleyrodidae in the Lowermost Eocene amber of Oise, France. The following new taxa in the subfamily Aleurodicinae are described, figured and discussed, together with an identification key: Oisedicus maginus gen. et sp. n., Clodionusfizoli gen. et sp. n., Lukotekia menae gen. et sp. n. and Isaraselis cladiva gen. et sp. n. Unplaced species of Lukotekia are briefly described, and the diversity of the whiteflies from Oise amber is discussed. The importance of fossils for palaeoecological and palaeoclimatological reconstruction is briefly considered.

  9. Eriocóccidos (Hemiptera: Coccoidea encontrados en Larrea spp. (Zygophyllaceae en Argentina

    Directory of Open Access Journals (Sweden)

    Patricia GONZÁLEZ

    2009-01-01

    Full Text Available Se describen e ilustran las hembras adultas de cuatroespecies nuevas de eriocóccidos: Eriococcus clapsae sp. nov., E. cuneifoliae sp. nov., E. divaricatae sp. nov. y E. pituilensis sp. nov. Ovaticoccus lahille (Leonardi se transfiere al género Eriococcus como E. lahillei (Leonardi, comb. nov., su hembra adulta y su primer estadio ninfal son redescriptos e ilustrados. Todas las especies de eriocóccidos arriba mencionados ocurren en Argentina y se encuentran en plantas del género Larrea.

  10. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

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    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  11. A review of the Oriental leafhopper genera Thampoa Mahmood and Guheswaria Thapa (Hemiptera: Cicadellidae: Typhlocybinae).

    Science.gov (United States)

    Hou, Xiao-Jiao; Zhang, Ya-Lin; Huang, Min

    2016-01-01

    The genus Guheswaria Thapa is recorded for the first time from China; two new species, Thampoa quinquemaculata sp. nov. and Guheswaria brevicula sp. nov. are described; one species, Guheswaria linguplata rec. nov. is newly recorded from China; and one new combination, Thampoa alata (Dworakowska, 1994) comb. nov. (from Guheswaria) is proposed. A checklist and updated keys to species of Thampoa and Guheswaria are provided.

  12. Two new species of Megacanthaspis Takagi (Hemiptera, Sternorrhyncha, Coccoidea, Diaspididae from China

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    Jiu-Feng Wei

    2012-07-01

    Full Text Available Two new species of armored scale, Megacanthaspis hangzhouensis Wei & Feng, sp. n. and M. hainanensis Wei & Feng, sp. n. are described and illustrated from specimens collected from China. A key to adult female of Megacanthaspis species is provided.

  13. Un género nuevo de Eriococcidae (Hemiptera: Coccoidea de la Argentina

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    Patricia GONZÁLEZ

    2008-01-01

    Full Text Available Se describe un género nuevo, Poliloculus gen. nov. Se brinda una clave de los géneros de Eriococcidae de la Argentina. Se describe e ilustra la hembra adulta de Poliloculus stipae sp. nov., encontrada sobre Stipa sp. en la Patagonia.

  14. Two new species of Fiorinia Targioni-Tozzetti (Hemiptera: Coccoidea: Diaspididae) from China.

    Science.gov (United States)

    Wei, Jiufeng; Zhang, Bin; Feng, Jinian

    2013-01-01

    The adult females of two new species of armored scale, Fiorinia tianshuiensis Wei & Feng sp. n. and F. dinghuensis Wei & Feng sp. n., are described and illustrated from specimens collected from China. A key is provided for the all Fiorilim species known from China.

  15. New genera and species of the tribe Taphurini (Hemiptera, Cicadidae) from Sundaland

    NARCIS (Netherlands)

    Duffels, J.P.

    2011-01-01

    Three new genera and five species of Taphurini from Sundaland (Malayan Peninsula, Sumatra, Borneo and Java) are described: Sundabroma including S. bimaculata sp. n. from Borneo and S. suffusca sp. n. from the Malayan Peninsula, Allobroma including A. kedenburgi (Breddin, 1905) comb. n. from Borneo a

  16. Characterization of a recombinant Cathepsin B-Like cysteine peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A putative target control of citrus huanglongbing

    Science.gov (United States)

    Huanglongbing (HLB) is one of the most destructive disease affecting citrus plants. The causal agent is associated with the phloem-limited bacterium Candidatus Liberibacter asiaticus (CLas) spread by the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae). Among the control strategies for H...

  17. Complete Genome Sequences of the Obligate Symbionts "Candidatus Sulcia muelleri" and "Ca. Nasuia deltocephalinicola" from the Pestiferous Leafhopper Macrosteles quadripunctulatus (Hemiptera: Cicadellidae).

    Science.gov (United States)

    Bennett, Gordon M; Abbà, Simona; Kube, Michael; Marzachì, Cristina

    2016-01-01

    Two bacterial symbionts of the European pest leafhopper, Macrosteles quadripunctulatus (Hemiptera: Cicadellidae), were fully sequenced. "Candidatus Sulcia muelleri" and "Ca. Nasuia deltocephalinicola" represent two of the smallest known bacterial genomes at 190 kb and 112 kb, respectively. Genome sequences are nearly identical to strains reported from the closely related host species, M. quadrilineatus.

  18. First record of the leafhopper genus Zyginopsis Ramakrishnan & Menon (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini) from China, with descriptions of two new species.

    Science.gov (United States)

    Jiang, Hao; Zhang, Yalin

    2016-01-01

    The erythroneurine leafhopper genus Zyginopsis Ramakrishnan & Menon (Hemiptera: Cicadellidae: Typhlocybinae) is reported for the first time from China, with descriptions and illustrations of two new species, Zyginopsis paraverticalis and Zyginopsis gracilis spp. nov., and one newly recorded species from China, Zyginopsis verticalis Ahmed n. rec. A key for identification of Chinese species (adult males) is provided.

  19. Characterization of EPG waveforms for the tea green leafhopper, Empoasca vitis Göthe (Hemiptera: Cicadellidae), on tea plants and their correlation with stylet activities

    Science.gov (United States)

    Stylet probing activities of the tea green leafhopper, Empoasca vitis Göthe (Hemiptera: Cicadellidae), were studied using the DC electrical penetration graph (EPG) technique. Seven different EPG waveforms (i.e., Np, E1, E2, E3, E4, E5 and E6) were identified and characterized on susceptible tea leav...

  20. Knockdown and lethal effects of eight commercial nonconventional and two pyrethroid insecticides against moderately permethrin-resistant adult bed bugs, Cimex lectularius (L.) (Hemiptera: Cimicidae)

    Science.gov (United States)

    The common bed bug, Cimex lectularius (L.) (Hemiptera: Cimicidae) is undergoing a rapid resurgence in the United States during the last decade which has created a notable pest management challenge largely because the pest has developed resistance against DDT, organophosphates, carbamates, and pyreth...

  1. Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psylidae) in Persian lime under field conditions

    Science.gov (United States)

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a destructive insect pest in the citriculture, because it is an efficient vector of the proteobacteria, ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. L. Africanus’ (Laf), and ‘Ca. L. Americanus’ (Lam). These bacteria c...

  2. Can we predict brown stink bug (Hemiptera: Pentatomidae) abundance in corn using previous pheromone trap capture and early season weather data?

    Science.gov (United States)

    Brown stink bug, Euschistus servus (Say) (Hemiptera: Pentatomidae), is a serious economic pest of corn production in the southern US. Scouting stink bugs in corn is time consuming and could be improved if scouts were aware of conditions that favored imminent stink bug infestations. Changes in seas...

  3. Praon volucre (Hymenoptera: Braconidae: Aphidiinae), a natural enemy of Macrosiphum euphorbiae (Hemiptera: Aphididae): Life table and intrinsic rate of population increase

    NARCIS (Netherlands)

    Lins, J.C.; Bueno, V.H.P.; Silva, D.B.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    Life table data of natural enemies are often used to understand their population dynamics and estimate their potential role in the biological control of pests. Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae) is an important pest of several crops and its intrinsic rate of population increase (

  4. A study of the scale insect genera Puto Signoret (Hemiptera: Sternorrhyncha: Coccoidea: Putoidae) and Ceroputo Šulc (Pseudococcidae) with a comparison to Phenacoccus Cockerell (Pseudococcidae)

    Science.gov (United States)

    For almost a century, the scale insect genus Puto Signoret (Hemiptera: Sternorrhyncha: Coccoidea) was considered to belong to the family Pseudococcidae (the mealybugs), but recent consensus accords Puto its own family, the Putoidae. This paper reviews the taxonomic history of Puto and family Putoida...

  5. Use of Electrical Penetration Graph Technology to Examine Transmission of ‘Candidatus Liberibacter solanacearum’ to Potato by Three Haplotypes of Potato Psyllid (Bactericera cockerelli; Hemiptera: Triozidae)

    Science.gov (United States)

    The potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae), is a vector of the phloem-limited bacterium ‘Candidatus Liberibacter solanacearum’ (Lso), the putative causal agent of zebra chip disease of potato. Little is known about how potato psyllid transmits Lso to potato. We used ele...

  6. Pre shipping dip treatments using soap, natural oils, and Isaria fumosorosea: potential biopesticides for mitigating the spread of whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) invasive insects on ornamental plants

    Science.gov (United States)

    The whitefly Bemisia tabaci (Hemiptera: Aleyodidae) is an invasive insect pest affecting different crops including vegetables, fruits, cereals, and ornamentals. The efficacy of some products such as commercial soap, natural oils and Preferal® (based on the entomopathogenic fungus Isaria fumosorosea ...

  7. Characterization of twelve novel microsatellite markers of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) identified from next generation sequence data

    Science.gov (United States)

    The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is a major pest of rice and has long-range migratory behavior in Asia. Microsatellite markers (simple sequence repeats, SSRs) have been widely used to determine the origins and genetic diversity of insect pests. ...

  8. Repellency of mustard (Brassica juncea) and arugula (Eruca sativa) plants, and plant oils against the sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

    Science.gov (United States)

    The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is an economic complex of at least 36 cryptic species, comprising a highly polyphagous and serious pest of vegetable, fiber and ornamental crops. Sustainable alternative measures such as cultural controls can be effective ...

  9. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil; Primeira ocorrencia de Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) predando lagartas desfolhadoras do dendezeiro no estado do Para, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A. [EMBRAPA Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia], e-mail: rafaufra@yahoo.com.br, e-mail: wplemos@cpatu.embrapa.br; Muller, Antonio A. [Embrapa Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia; Bernardino, Aline S.; Buecke, Joel [Grupo Agropalma S/A., Tailandia, PA (Brazil)

    2010-01-15

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  10. Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha

    Directory of Open Access Journals (Sweden)

    Baumann Paul

    2004-08-01

    Full Text Available Abstract Background With some exceptions, mitochondria within the class Insecta have the same gene content, and generally, a similar gene order allowing the proposal of an ancestral gene order. The principal exceptions are several orders within the Hemipteroid assemblage including the order Thysanoptera, a sister group of the order Hemiptera. Within the Hemiptera, there are available a number of completely sequenced mitochondrial genomes that have a gene order similar to that of the proposed ancestor. None, however, are available from the suborder Sternorryncha that includes whiteflies, psyllids and aphids. Results We have determined the complete nucleotide sequence of the mitochondrial genomes of six species of whiteflies, one psyllid and one aphid. Two species of whiteflies, one psyllid and one aphid have mitochondrial genomes with a gene order very similar to that of the proposed insect ancestor. The remaining four species of whiteflies had variations in the gene order. In all cases, there was the excision of a DNA fragment encoding for cytochrome oxidase subunit III(COIII-tRNAgly-NADH dehydrogenase subunit 3(ND3-tRNAala-tRNAarg-tRNAasn from the ancestral position between genes for ATP synthase subunit 6 and NADH dehydrogenase subunit 5. Based on the position in which all or part of this fragment was inserted, the mitochondria could be subdivided into four different gene arrangement types. PCR amplification spanning from COIII to genes outside the inserted region and sequence determination of the resulting fragments, indicated that different whitefly species could be placed into one of these arrangement types. A phylogenetic analysis of 19 whitefly species based on genes for mitochondrial cytochrome b, NADH dehydrogenase subunit 1, and 16S ribosomal DNA as well as cospeciating endosymbiont 16S and 23S ribosomal DNA indicated a clustering of species that corresponded to the gene arrangement types. Conclusions In whiteflies, the region of the

  11. A two-in-one superhydrophobic and anti-reflective nanodevice in the grey cicada Cicada orni (Hemiptera)

    Energy Technology Data Exchange (ETDEWEB)

    Dellieu, Louis, E-mail: louis.dellieu@unamur.be; Sarrazin, Michaël, E-mail: michael.sarrazin@unamur.be; Simonis, Priscilla; Deparis, Olivier; Vigneron, Jean Pol [Research Center in Physics of Matter and Radiation (PMR), Department of Physics, University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur (Belgium)

    2014-07-14

    Two separated levels of functionality are identified in the nanostructure which covers the wings of the grey cicada Cicada orni (Hemiptera). The upper level is responsible for superhydrophobic character of the wing, while the lower level enhances its anti-reflective behavior. Extensive wetting experiments with various chemical species and optical measurements were performed in order to assess the bi-functionality. Scanning electron microscopy imaging was used to identify the nanostructure morphology. Numerical optical simulations and analytical wetting models were used to prove the roles of both levels of the nanostructure. In addition, the complex refractive index of the chitinous material of the wing was determined from measurements.

  12. First Report of Citrus Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae in the State of Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2014-08-01

    Full Text Available The citrus blackfly Aleroucanthus woglumi Ashby, (Hemiptera Aleyrodidae is an important pest that occurs in citrus groves, native to south-east Asia. In Brazil, according to the Ministry of Agriculture, this is a quarantine pest (A2 under official control IN 52, 2007 (MAPA and is not widespread in the country. The insect can infest more than 300 host plants, including cultivated plants, ornamentals and weeds, but mostly occurs in the plants of the genus citrus. This paper is the first report of citrus blackfly in the State of Paraná.

  13. [Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Tratominae) in the Brazilian State of Rio Grande do Sul].

    Science.gov (United States)

    Salvatella Agrelo, R; Basmadjian, Y; Rosa, R; Puime, A

    1993-01-01

    Triatoma delpontei (Romaña & Abalos, 1947) (Hemiptera, Triatominae) is an ornithophilic sylvatic with a particular association to the psittacid Myiopsitta monachus (Boaddert, 1783). It is found in the continental biogeographical province of the Chaco, where it inhabits the nests or M. monachus, in subtropical xerophytic forests. The authors report the first finding of T. delpontei in Brasil, in the "campanha" region of the State or Rio Grande do Sul (Barra do Quarai, Uruguaiana), on the right bank of the River Cuareim, not far from de Uruguayan border.

  14. Hallazgo de Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae) en el estado brasileño de "Rio Grande do Sul"

    OpenAIRE

    Salvatella,Roberto; Basmadjián,Yester; Rosa,Raquel; Martínez, María; Mendaro,Gabriela; Civila,Eduardo

    1991-01-01

    Triatoma platensis Neiva 1913 (Hemiptera, Triatominae), especie ornitófila, con área de dispersión conocida en Argentina, Bolivia, Paraguay y Uruguay es notificada en un primer registro para Brasil. Hallada en el municipio de Uruguaiana (Río Grande do Sul) en nidos de Anumbius annumbi (Vieillot, 1817) (Passeriformes, Furnariidae) donde cohabitaba con cricétidos de la especie Orizomys flavescens. Ningún ejemplar de los siete colectados se presentó infectado por Trypanosoma cruzi.

  15. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Science.gov (United States)

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  16. An annotated checklist of the scale insects of Iran (Hemiptera, Sternorrhyncha, Coccoidea with new records and distribution data

    Directory of Open Access Journals (Sweden)

    Masumeh Moghaddam

    2013-09-01

    Full Text Available A list of scale insects (Hemiptera: Sternorrhyncha: Coccoidea of Iran is present based mainly on the literature records since 1902. In total, 13 families and 275 species have been recorded and these are listed along with their locality data and host plants. The families are as follows: Asterolecaniidae, Cerococcidae, Coccidae, Diaspididae, Eriococcidae, Kermesidae, Margarodidae, Monophlebidae, Ortheziidae, Phoenicococcidae, Pseudococcidae, Putoidae and Rhizoecidae. The following ten species are recorded for the first time from Iran: Diaspidiotus lenticularis (Lindinger, D. wuenni (Lindinger, Fiorinia proboscidaria Green, Koroneaspis lonicerae Borchsenius, Eriococcus cingulatus Kiritchenko, E. pamiricus (Bazarov, E. reynei Schmutterer, E. sanguinairensis Goux and E. saxidesertus (Borchsenius and Porphyrophora victoriae Jashenko.

  17. An annotated checklist of the scale insects of Iran (Hemiptera, Sternorrhyncha, Coccoidea) with new records and distribution data.

    Science.gov (United States)

    Moghaddam, Masumeh

    2013-01-01

    A list of scale insects (Hemiptera: Sternorrhyncha: Coccoidea) of Iran is present based mainly on the literature records since 1902. In total, 13 families and 275 species have been recorded and these are listed along with their locality data and host plants. The families are as follows: Asterolecaniidae, Cerococcidae, Coccidae, Diaspididae, Eriococcidae, Kermesidae, Margarodidae, Monophlebidae, Ortheziidae, Phoenicococcidae, Pseudococcidae, Putoidae and Rhizoecidae. The following ten species are recorded for the first time from Iran: Diaspidiotus lenticularis (Lindinger), Diaspidiotus wuenni (Lindinger), Fiorinia proboscidaria Green, Koroneaspis lonicerae Borchsenius, Eriococcus cingulatus Kiritchenko, Eriococcus pamiricus (Bazarov), Eriococcus reynei Schmutterer, Eriococcus sanguinairensis Goux and Eriococcus saxidesertus (Borchsenius) and Porphyrophora victoriae Jashenko.

  18. Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments.

    Science.gov (United States)

    den Bakker, Henk C; Warchocki, Steven; Wright, Emily M; Allred, Adam F; Ahlstrom, Christina; Manuel, Clyde S; Stasiewicz, Matthew J; Burrell, Angela; Roof, Sherry; Strawn, Laura K; Fortes, Esther; Nightingale, Kendra K; Kephart, Daniel; Wiedmann, Martin

    2014-06-01

    Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T) = DSM 26686(T) = LMG 28120(T) = BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T) = DSM 26687(T) = LMG 28121(T) = BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T) = FSL F6-0969(T) = DSM 26689(T) = LMG 28123(T) = BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T) = FSL F6-0971(T) = DSM 26688(T) = LMG 28122(T) = BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T) = DSM 26685(T) = LMG 28119(T) = BEI NR- 42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic.

  19. Toxicity of insecticides to the sweetpotato whitefly (Hemiptera: Aleyrodidae) and its natural enemies.

    Science.gov (United States)

    Bacci, Leandro; Crespo, André L B; Galvan, Tederson L; Pereira, Eliseu J G; Picanço, Marcelo C; Silva, Gerson A; Chediak, Mateus

    2007-07-01

    Efficient chemical control is achieved when insecticides are active against insect pests and safe to natural enemies. In this study, the toxicity of 17 insecticides to the sweetpotato whitefly, Bemisia tabaci (Gennadius), and the selectivity of seven insecticides to natural enemies of this insect pest were evaluated. To determine the insecticide toxicity, B. tabaci adults were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion, methamidophos, bifenthrin, cypermethrin, deltamethrin, esfenvalerate, fenitrothion, fenpropathrin, fenthion, phenthoate, permethrin and trichlorphon at 50 and 100% of the field rate (FR), and to water (untreated control). To determine the insecticide selectivity, adults of Encarsia sp., Acanthinus sp., Discodon sp. and Lasiochilus sp. were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion and methamidophos at 50 and 100% FR, and to water. Groups of each insect species were exposed to kale leaves preimmersed in each treatment under laboratory conditions. Mortality of exposed individuals was recorded 24 h after treatment. Cartap and imidacloprid at 50 and 100% FR and abamectin and acetamiprid at 100% FR showed insecticidal activity to B. tabaci adults. Abamectin at 50 and 100% FR was the least insecticidal compound to the natural enemies Acanthinus sp., Discodon sp. and Lasiochilus sp. The present results suggest that abamectin at 100% FR may decrease B. tabaci field populations but can still be harmless to predators. Implications of these results within an integrated pest management context are discussed.

  20. Feeding preference ofNezara viridula (Hemiptera: Pentatomidae and attractiveness of soybean genotypes

    Directory of Open Access Journals (Sweden)

    Efrain de Santana Souza

    2013-12-01

    Full Text Available Nezara viridula (L. (Hemiptera: Pentatomidae is a cosmopolitan insect that causes economic damages to several cultures, in particular soybeans (Glycine max [L.] Merr. Among the techniques that involve Integrated Pest Management, the resistance of plants is pointed as a tool of great value and can contribute to the reduction of populations of insects. The feeding preferences of adults of southern green stink bug (N. viridula, and the attractiveness of soybean genotypes were evaluated under laboratory conditions to detect the most resistant material against attack from this insect. A choice test, using mature grains and green pods of the genotypes was carried out, in which the number of individuals attracted in different periods was counted. Feeding preference was evaluated in the choice tests using green pods and the number of pricks and the average time spent feeding by pricks were evaluated. In addition, texture and trichome density in the green pods were evaluated. The mature grains of 'TMG 117RR' and 'TMG 121RR' were less attractive to the adults of N. viridula. Regarding the green pods, 'IAC 17' and PI 227687 were less attractive; 'IAC 17' and 'IAC PL1' were less consumed, indicating the feeding non-preference as a resistance mechanism. 'IAC 17', 'TMG-117RR' and PI 227687 presented high levels of trichome density, and in 'IAC 17' this morphological characteristic was considered to be the main resistance factor against N. viridula. These results may be useful for breeding programs that focus on the resistance of soybeans to insects.

  1. Ecology and Management of Kudzu Bug (Hemiptera: Plataspidae) in Southeastern Soybeans

    Science.gov (United States)

    Lahiri, Sriyanka

    2016-01-01

    Kudzu bug, Megacopta cribraria Fabricius (Hemiptera: Plataspidae), is an invasive exotic pest of soybeans that has been present in the southeastern United States since 2009 and has been rapidly spreading through soybean-producing states. Their primary reproductive hosts in the United States are soybean, kudzu, pigeon pea, black eye pea, lima bean, pinto bean, wisteria, white sweet clover, white clover, red clover, alfalfa, perennial peanut, and American joint vetch. In soybeans, the kudzu bug feeds on vascular fluids at the stem, petiole, and nodes, causing yield losses of up to 60%. The current management recommendation for this pest includes spraying of pyrethroids such as bifenthrin, but this method is not environmentally friendly, as this negatively impacts beneficial insect populations. Sustainable management tactics, including the development of economic thresholds for insecticide sprays, assessing the spatial and temporal distribution of this pest, manipulating cultivation practices, use of biological control, and host plant resistance, are currently being explored. We present an overview of the ecology of the kudzu bug in soybeans and available management tactics to assist with the management of this potentially devastating pest of soybeans as it spreads westward.

  2. First record of Triatoma maculata (Erichson, 1848 (Hemiptera: Reduviidae: Triatomini in Riohacha, La Guajira – Colombia

    Directory of Open Access Journals (Sweden)

    Edith Natalia Gómez-Melendro

    2014-11-01

    Full Text Available Introduction. Knowledge of vector insect species, their habitat and geographical distribution is crucial for determining the risk of transmission of the etiologic agents that cause disease in humans, which allows defining strategies for prevention, surveillance and control in line with the characteristics of each area. Objective. To determine the presence and public health importance of vectors of Chagas disease in the indigenous settlements of Marbacella and El Horno of the Wayúu ethnic group in the municipality of Riohacha, La Guajira, Colombia. Materials and methods. From active search, installation and inspection of biosensors and occasional catches, Hemiptera: Reduviidae: Triatomini were collected intra and in the peridomicile housing of the indigenous settlements of El Horno and Marbacella of the the Wayúu ethnic group. Indices of intra and peridomestic infestation, colonization, density, dispersion and natural infection with Trypanosoma cruzi Chagas, 1909 were calculated. Results. 79.6% (n = 90 of the specimens were collected around the homes and 20.3% (n = 23 inside the homes, all corresponding to Triatoma maculata (Erichson, 1848. The natural infection indices with T. cruzi accounted for 43.5% for Marbacella and 36% for El Horno. Conclusion. This is the first reported capture of individuals of T. maculata, considered a secondary vector of Chagas disease in Colombia, naturally infected with T. cruzi in the municipality of Riohacha expanding the geographical distribution of the species in the department of La Guajira.

  3. Molecular characterization and gene functional analysis of Dicer-2 gene from Nilaparvata lugens(Hemiptera:Geometroidea)

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yi Zhang; Kai Lu; Jia-Liang Zhou; Qiang Zhou

    2013-01-01

    Nilaparvata lugens(St((A)l)(Hemiptera: Geometroidea),a serious rice pest in many countries of Asia,causes a great loss in rice production every year.RNA interference (RNAi)is a powerful technology for gene function study in insects and a potential tool for pest control.As a core component of RNAi pathway,Dicer-2(Dcr-2)protein determines the production of small interfering RNA(siRNA)and is crucial for the efficiency of RNAi.In this study,the full-length complementary DNA(cDNA)ofN.lugens Dcr-2(NlDcr-2)was first cloned and analyzed,and then the RNAi experiment was conducted to explore the function of NIDcr-2 gene.The complete Dcr-2 cDNA ofN.lugens was 4971 bp in length with an open reading frame(ORF)of 1,656 amino acids.Phylogenetic and protein domain analysis showed that the predicted NlDcr-2 protein was similar to Tribolium castaneum.In the RNAi experiment,the messenger RNA level of NIDcr-2 was significantly reduced by NlDcr-2double-stranded RNA(dsRNA)(dsDcr-2).Fifty-five per cent decrease of NlDcr-2 was found after 4 days of unremitting feeding.No significant effect was observed on the development ofN.lugens after dsRNA ingestion.

  4. Inter- and intraspecific variation in defensive compounds produced by five neotropical stink bug species (Hemiptera: Pentatomidae).

    Science.gov (United States)

    Pareja, Martín; Borges, Miguel; Laumann, Raúl A; Moraes, Maria C B

    2007-07-01

    The differences in composition of defensive secretions between nymphs, adult males and adult females of Chinavia impicticornis (=Acrosternum impicticorne), Chinavia ubica (=Acrosternum ubicum), Euschistus heros, Dichelops melacanthus and Piezodorus guildinii (Hemiptera, Pentatomidae) were analysed within and between species using compositional log-ratio statistics and canonical variates analysis. Differences in composition between nymphs, males and females were found for all species, as well as when all species were pooled. In particular, tetradecanal appears to be a predominantly nymphal compound in D. melacanthus, E. heros and P. guildinii. In the two Chinavia species 4-oxo-(E)-2-hexenal and an unknown compound were more dominant in nymphs. The interspecific analysis revealed a good separation of defensive compounds according to their taxonomic relationship. Thus, the two Chinavia species grouped together, with (E)-2-decenal and (E)-2-hexenyl acetate, contributing to this separation. The other three species also differed from each other, with (E)-2-octenal associated to D. melacanthus, (E)-2-hexenal to P. guildinii and (E,E)-2,4-decadienal and tetradecanal to E. heros. The pooled analysis of stage ignoring species revealed tetradecanal and 4-oxo-(E)-2-decenal (tentative identification) strongly associated to nymphs. Thus, there are predictable differences between stages, and many of the differences are conserved between species. Consideration of these differences could prove to be important in understanding stink bug-natural enemy interactions, and in optimising biocontrol efforts.

  5. Relative Infestation Level and Sensitivity of Grapevine Cultivars to the Leafhopper Empoasca vitis (Hemiptera: Cicadellidae).

    Science.gov (United States)

    Fornasiero, D; Pavan, F; Pozzebon, A; Picotti, P; Duso, C

    2016-02-01

    The leafhopper Empoasca vitis (Göthe) (Hemiptera: Cicadellidae) feeds on veins of grapevine leaves, mainly on the phloem, causing physiological injury, color change and drying of leaf margins, yield and sugar content reduction. The relative infestation level (i.e., the probability that a plant is attacked by herbivores) of E. vitis on different grapevine cultivars and their sensitivity (i.e., the incidence of symptoms expression in response to herbivore feeding or other stimuli) to this pest were studied over four years in two vineyards located in northeastern Italy. Some cultivars (e.g., Carménère and Sauvignon Blanc) were usually more infested than others (e.g., Cabernet Sauvignon and Pinot Gris), although some differences were observed among years and sites. The sensitivity varied among cultivars, i.e., some of them showed more symptoms than expected on the basis of infestation levels (e.g., Carménère and Merlot), in contrast with others (e.g., Rhine Riesling and Chardonnay). Information obtained can be used within the framework of integrated pest management in vineyards. Action thresholds should differ on the basis of sensitivity. Sampling must first be carried out on the most susceptible cultivar and, if the action threshold is exceeded, it should be extended to the remaining cultivars based on their decreasing relative infestation level.

  6. Morphometric comparisons of Diaphorina citri (Hemiptera: Liviidae populations from Iran, USA and Pakistan

    Directory of Open Access Journals (Sweden)

    Mohammadreza Lashkari

    2015-05-01

    Full Text Available The Asian citrus psyllid (ACP, Diaphorina citri Kuwayama (Hemiptera: Liviidae, vector of citrus greening disease pathogen, Huanglongbing (HLB, is considered the most serious pest of citrus in the world. Prior molecular based studies have hypothesized a link between the D. citri in Iran and the USA (Florida. The purpose of this study was to collect morphometric data from D. citri populations from Iran (mtCOI haplotype-1, Florida (mtCOI haplotype-1, and Pakistan (mtCOI haplotype-6, to determine whether different mtCOI haplotypes have a relationship to a specific morphometric variation. 240 samples from 6 ACP populations (Iran—Jiroft, Chabahar; Florida—Ft. Pierce, Palm Beach Gardens, Port St. Lucie; and Pakistan—Punjab were collected for comparison. Measurements of 20 morphological characters were selected, measured and analysed using ANOVA and MANOVA. The results indicate differences among the 6 ACP populations (Wilks’ lambda = 0.0376, F = 7.29, P < 0.0001. The body length (BL, circumanal ring length (CL, antenna length (AL, forewing length (WL and Rs vein length of forewing (RL were the most important characters separating the populations. The cluster analysis showed that the Iran and Florida populations are distinct from each other but separate from the Pakistan population. Thus, three subgroups can be morphologically discriminated within D. citri species in this study, (1 Iran, (2 USA (Florida and (3 Pakistan population. Morphometric comparisons provided further resolution to the mtCOI haplotypes and distinguished the Florida and Iranian populations.

  7. Evaluation of digital photography for quantifying Cryptococcus fagisuga (Hemiptera: Eriococcidae) density on American beech trees.

    Science.gov (United States)

    Wieferich, D J; Hayes, D B; McCullough, D G

    2013-06-01

    Beech scale (Cryptococcus fagisuga Lindinger) (Hemiptera: Eriococcidae) is an invasive forest insect established in the eastern United States and Canada. It predisposes American beech (Fagus grandifolia Ehrhart) trees to infection by Neonectria spp. Fungi causing beech bark disease. White wax secreted by the diminutive scales obscures individual insects, making it difficult to accurately quantify beech scale density. Our goals were to 1) evaluate the relationship between the area of wax and number of beech scales on bark samples, 2) determine whether digital photos of bark could accurately quantify beech scale density, and 3) compare efficiency and utility of a qualitative visual estimate and using the quantitative digital photo technique to assess beech scale populations. We visually estimated beech scale abundance and photographed designated areas on the trunk of 427 trees in 40 sites across Michigan. Photos were analyzed using a binary threshold technique to quantify the area of beech scale wax on each photo. We also photographed and then collected 104 bark samples from 45 additional beech trees in ten sites. We removed the wax, counted individual scales on each sample using a microscope, and assessed the linear relationship between wax area and scale counts. Area of wax explained approximately 80% of the variability in scale density. We could typically quantify beech scale density on 15 photographs per hour. Qualitative visual assessments of beech scale in the field corresponded with estimates derived from photos of bark samples for 79% of trees.

  8. Probing behaviors of Sitobion avenae (Hemiptera: Aphididae on enhanced UV-B irradiated plants

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    Hu Zu-Qing

    2013-01-01

    Full Text Available UV-B induced changes in plants can influence sap-feeding insects through mechanisms that have not been studied. Herein the grain aphid, Sitobion avenae (Fabricius (Hemiptera: Aphididae, was monitored on barley plants under the treatments of control [0 kJ/ (m2.d], ambient UV-B [60 kJ/ (m2.d], and enhanced UV-B [120 kJ/ (m2.d] irradiation. Electrical penetration graph (EPG techniques were used to record aphid probing behaviors. Enhanced UV-B irradiated plants negatively affected probing behaviors of S. avenae compared with control plants. In particular, phloem factors that could diminish sieve element acceptance appeared to be involved, as reflected by smaller number of phloem phase, shorter phloem ingestion, and fewer aphids reaching the sustained phloem ingestion phase (E2>10min. On the other hand, factors from leaf surface, epidermis, and mesophyll cannot be excluded, as reflected by higher number of non-probing, longer non-probing and pathway phase, and later the time to first probe.

  9. Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae by RAPD-PCR technique

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    Jorge Fraga

    2011-02-01

    Full Text Available The Triatominae (Hemiptera:Reduviidae contains the principal and potential Chagas disease vectors present in Mexico, Central America and South America. Triatoma flavida and T. bruneri are Cuban species. These species are closely related according to morphology and were considered synonyms until 1981, when they were separated on the grounds of external characters of the body and the morphology of male genitalia. The present study seeks to analyze genetic polymorphism of T. flavida and T. bruneri populations using RAPD techniques, and to assess the genetic relationship between these species. Ten random primers were used to evaluate the genetic variability among species using RAPD-PCR. The genetic flow among them was calculated. The dendrogram based on calculated Jaccard distances showed two clearly distinguishable clusters which coincided with the studied species. Within each species, moderate genetic differentiation (Fst 0.05-0.15 and migration rates (N > 1 were found among populations, that reveal gene flow and genetic homogeneity. Between species, the Fst value showed a high genetic differentiation and the migration rate was insufficient to maintain genetic homogeneity, and confirmed the absence of gene flow between them. Our results confirm the genetic variability among T. flavida and T. bruneri species.

  10. Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique.

    Science.gov (United States)

    Fraga, Jorge; Rodriguez, Jinnay; Fuentes, Omar; Hernández, Yenin; Castex, Mayda; Gonzalez, Raul; Fernández-Calienes, Aymé

    2011-01-01

    The Triatominae (Hemiptera:Reduviidae) contains the principal and potential Chagas disease vectors present in Mexico, Central America and South America. Triatoma flavida and T. bruneri are Cuban species. These species are closely related according to morphology and were considered synonyms until 1981, when they were separated on the grounds of external characters of the body and the morphology of male genitalia. The present study seeks to analyze genetic polymorphism of T. flavida and T. bruneri populations using RAPD techniques, and to assess the genetic relationship between these species. Ten random primers were used to evaluate the genetic variability among species using RAPD-PCR. The genetic flow among them was calculated. The dendrogram based on calculated Jaccard distances showed two clearly distinguishable clusters which coincided with the studied species. Within each species, moderate genetic differentiation (Fst 0.05-0.15) and migration rates (N > 1) were found among populations, that reveal gene flow and genetic homogeneity. Between species, the Fst value showed a high genetic differentiation and the migration rate was insufficient to maintain genetic homogeneity, and confirmed the absence of gene flow between them. Our results confirm the genetic variability among T. flavida and T. bruneri species.

  11. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae

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    Samman Fatima

    2016-01-01

    Full Text Available Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae, is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14% of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83% was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  12. Harmonic radar tagging for tracking movement of Nezara viridula (Hemiptera: Pentatomidae).

    Science.gov (United States)

    Pilkay, Grant L; Reay-Jones, Francis P F; Greene, Jeremy K

    2013-10-01

    Harmonic radar tagging was investigated as a method for monitoring the movement of the southern green stink bug, Nezara viridula (L.) (Hemiptera: Pentatomidae). Because adhesive toxicity and tag weight limit the use of this technology, initial efforts focused on selection of the optimal adhesive and design of harmonic radar tags to reduce impact on the movement of stink bugs. A design consisting of a 6-cm-long 0.10-mm-thick silver-plated copper monopole on the anode terminal of a three-contact Schottky barrier diode attached with Gorilla super glue provided a compromise between unimpaired movement and tracking range, adding an additional 8% to the weight of the stink bug while not significantly (P > 0.05) reducing walking or flying mobility in the laboratory. Recovery of tagged stink bugs in cotton, Gossypium hirsutum (L.), and fallow fields ranged from 10 to 75% after 24 h, whereas marked stink bugs were recovered at rates of 0-35% by using sweep net or drop cloth sampling. The distance dispersed in the field was not impacted (P > 0.05) by crop, tagged status, or gender of the insect. Future research should examine possible improvements to the harmonic radar transceiver and the wire antenna to decrease encumbrance.

  13. Effect of population structure and size on aggregation behavior of Cimex lectularius (Hemiptera: Cimicidae).

    Science.gov (United States)

    Pfiester, Margie; Koehler, Philip G; Pereira, Roberto M

    2009-09-01

    The bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), occurs in aggregations until the conditions are no longer beneficial, leading to dispersal. Active and passive bed bug dispersal causes migrations from main aggregations either within a room, from room to room within a building, or from building to building. Because bed bug movement is an important factor in the spread of infestations, we wanted to determine how population structure and size affect bed bug aggregations. Engorged bed bugs were placed in glass petri dish arenas at varying densities, sex ratios, and population compositions. Nymphs had a high tendency to aggregate, varying between 94 and 98%, and therefore were not the likely dispersal stage of the bed bug. At densities of 10 and 40 adults at a 1:1 sex ratio, there were significantly more lone females than lone males. When the population composition was varied, the percentage of lone females was significantly higher than that of males and nymphs at population compositions of 40 and 80% adults. When the sex ratio of adults was varied, there were significantly more lone females than males in arenas with 20, 50, and 80% males. Females, being found away from aggregations significantly more often than any other life stage, are potentially the dispersal stage of the bed bug. Active female dispersal away from main aggregations can potentially lead to treatment failures and should be taken into account when using control methods.

  14. Effects of fertilizer and low rates of imidacloprid on Adelges tsugae (Hemiptera: Adelgidae).

    Science.gov (United States)

    Joseph, S V; Hanula, J L; Braman, S K; Byrne, F J

    2011-06-01

    Healthy hemlock trees, Tsuga canadensis (L.) Carrière, and hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), populations should favor retention and population growth of adelgid predators such as Laricobius nigrinus Fender (Coleoptera: Derodontidae) and Sasajiscymnus tsugae (Sasaji & McClure) (Coleoptera: Coccinellidae). Eastern hemlock trees between 15 and 38 cm diameter at breast height (dbh) were treated with 0, 10, or 25% of 1.5 g imidacloprid (Merit 75 WP) per 2.5 cm dbh and were either fertilized or not, in a 3 by 2 factorial design. After 2 yr, imidacloprid reduced the numbers of ovisacs and eggs found on trees in a dosage-dependent manner, while enhancing tree growth parameters such as new shoots or needles and the length of new shoots. Fertilized trees had greater adelgid fecundity, which was positively correlated with total foliar N in both winter generations. In February 2009 (27 mo after imidacloprid treatment), higher imidacloprid dosages to unfertilized trees resulted in reduced adelgid fecundity. Concentrations of N, P, and K were higher in the foliage of trees treated with insecticide, whereas foliar aluminum concentrations were consistently lower in trees with higher insecticide dosages. Trees treated with low rates of imidacloprid were healthier than untreated trees, but only trees treated with the 0.1 x dosage had sufficient adelgids to possibly sustain predators over extended periods.

  15. Effects of gamma irradiation on different stages of mealybug Dysmicoccus neobrevipes (Hemiptera: Pseudococcidae)

    Energy Technology Data Exchange (ETDEWEB)

    The, Doan Thi, E-mail: doanthithe@yahoo.com [Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, 202A Street 11, Linh Xuan Ward, Thu Duc District, Ho Chi Minh City (Viet Nam); Khanh, Nguyen Thuy; Lang, Vo Thi Kim; Van Chung, Cao [Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, 202A Street 11, Linh Xuan Ward, Thu Duc District, Ho Chi Minh City (Viet Nam); An, Tran Thi Thien; Thi, Nguyen Hoang Hanh [Agriculture and Forestry University, Linh Trung Ward, Thu Duc District, Ho Chi Minh City (Viet Nam)

    2012-01-15

    Utilization of phytosanitary irradiation as a potential treatment to disinfest agricultural commodities in trade has expanded rapidly in the recent years. Cobalt-60 gamma ray target doses of 100, 150, 200 and 250 Gy were used to irradiate immatures and adults of Dysmicoccus neobrevipes (Beardsley) (Hemiptera: Pseudococcidae) infesting dragon fruits to find the most tolerant stage and the most optimal dose range for quarantine treatment. In general, irradiation affected significantly all life stages of D. neobrevipes mortality and adult reproduction. The pattern of tolerance to irradiation in D. neobrevipes was 1st instars<2nd instars<3rd instars Gamma Co-60 irradiation as a potential phytosanitary for quarantine treatments. > Reproduction ability of D. neobrevipes has been efficiently inhibited at low dose. > Pattern of tolerance to irradiation was 1st<2nd<3rd instars Doses from 200 to 250 Gy could be efficient to prevent the reproduction of mealybug.

  16. Taxonomic corrections to species of Rhyparochromidae (Hemiptera: Heteroptera) described by Carl Peter Thunberg.

    Science.gov (United States)

    Kondorosy, Előd; Rédei, Dávid; Mejlon, Hans

    2014-07-22

    Types of Rhyparochromidae (Hemiptera: Heteroptera: Lygaeoidea) species described by Carl Peter Thunberg, deposited in the Museum of Evolution (formerly Zoologiska Institut), Uppsala University, Uppsala, Sweden, were reexamined and the taxonomic and nomenclatural problems that existed among those species discussed and resolved as required. Lectotypes are designated for Cimex caffer Thunberg, 1784, Lygaeus ater Thunberg, 1822, Lygaeus biguttatus Thunberg, 1822, and Pendulinus guttatus Thunberg, 1825. The lectotype of Pendulinus (now Metochus) guttatus is designated as neotype of Pendulinus (now Metochus) uniguttatus Thunberg, 1822; as a result the former name becomes junior objective synonym of the latter. The following taxonomic changes are proposed: Lethaeus ater (Thunberg, 1822), new combination (from Lygaeus); Migdilybs biguttatus (Thunberg, 1822), new combination (from Lygaeus) = Migdilybs furcifer Hesse, 1925, new subjective synonym; Metochus uniguttatus (Thunberg, 1822) = Metochus bengalensis (Dallas, 1852), confirmed subjective synonym = Metochus yeh (Dohrn, 1860), confirmed subjective synonym; Raglius alboacuminatus (Goeze, 1778) = Cimex caffer Thunberg, 1874, confirmed subjective synonym. Lethaeus barberi Slater, 1964 does not belong to Lethaeus Dallas, 1852 but currently it cannot be placed with confidence in any existing genus. 

  17. Effects of selected fertilizers on the life history of Bemisia tabaci (Hemiptera: Aleyrodidae) biotype B.

    Science.gov (United States)

    England, K M; Sadof, C S; Cañnas, L A; Kuniyoshi, C H; Lopez, R G

    2011-04-01

    We tested the effects among a purportedly sustainable water-soluble fertilizer, a conventional water-soluble fertilizer, an alternation of these, a controlled-release fertilizer, and a clear water control on the life-history traits of sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae; =Bemisia argentifolii Bellows & Perring) biotype B reared on poinsettia (Euphorbia pulcherrima Willdenow ex Klotzch). Free amino acids in petioles were measured to estimate plant nutrient assimilation and phloem nutritional quality for B. tabaci biotype B. The sustainable fertilizer produced plants with the highest concentration of amino acids. In contrast, fecundity of whiteflies was lowest in plants treated with the sustainable fertilizer and the water control. The relationship between total amino acids in phloem and survival was significantly quadratic, with the highest survival at intermediate levels. Fecundity, however, was negatively correlated with total amino acid content of the maternal host plant. Variation in total amino acid concentration in petioles of plants treated within fertilizer treatments makes it difficult to predict whether a particular fertilizer will produce plants with enough amino acids to deleteriously affect both survivorship and fecundity and yet yield a plant of good quality. Despite this limitation, we can conclude that the use of this sustainable fertilizer will not cause increases in whitefly populations relative to plants fertilized with water-soluble and slow-release fertilizers that deliver the same level of nitrogen to the plant.

  18. Dose-response relationships of clothianidin, imidacloprid, and thiamethoxam to Blissus occiduus (Hemiptera: Blissidae).

    Science.gov (United States)

    Stamm, M D; Baxendale, F P; Heng-Moss, T M; Siegfried, B D; Blankenship, E E; Gaussoin, R E

    2011-02-01

    The western chinch bug, Blissus occiduus Barber (Hemiptera: Blissidae), has emerged as a serious pest of buffalograss, Buchlod dactyloides (Nuttall) Engelmann. In general, neonicotinoid insecticides effectively control a variety of turfgrass insects, particularly phloem-feeding pests. However, because of well documented inconsistencies in control, these compounds are generally not recommended for chinch bugs. This study was designed to document the contact and systemic toxicity of three neonicotinoid insecticides (clothianidin, imidacloprid, and thiamethoxam) to B. occiduus. In contact bioassays, thiamethoxam was approximately 20-fold less toxic than clothianidin or imidacloprid to B. occiduus nymphs and three-fold more toxic to adults. In adult systemic bioassays, thiamethoxam was up to five-fold more toxic than clothianidin or imidacloprid. Interestingly, thiamethoxam was significantly more toxic to adults than to nymphs in both contact and systemic bioassays. This was not observed with clothianidin or imidacloprid. Bifenthrin, used for comparative purposes, exhibited 1844-fold and 122-fold increase in toxicity to nymphs and adults, respectively. These results provide the first documentation of the relative toxicity of these neonicotinoid insecticides to B. occiduus.

  19. Biotypic diversity in greenbug (Hemiptera: Aphididae): microsatellite-based regional divergence and host-adapted differentiation.

    Science.gov (United States)

    Weng, Yiqun; Perumal, Azhaguvel; Burd, John D; Rudd, Jackie C

    2010-08-01

    Nineteen isolates of the cereal aphid pest greenbug, Schizaphis graminum (Rondani) (Hemiptera: Aphididae), were collected from wheat, Triticum aestivum L.; barley, Hordeum vulgare L.; or noncultivated grass hosts in five locations from Colorado and Wyoming. Parthenogenetic colonies were established. Biotypic profiles of the 19 isolates were determined based on their abilities to damage a set of host plant differentials, and 13 new biotypes were identified. Genetic diversity among the 19 isolates and five previously designated greenbug biotypes (E, G, H, I, and K) was examined with 31 cross-species transferable microsatellite (simple sequence repeat) markers. Neighbor-joining clustering analysis of marker data revealed host-adapted genetic divergence as well as regional differentiation of greenbug populations. Host associated biotypic variation seems to be more obvious in "agricultural biotypes," whereas isolates collected from noncultivated grasses tend to show more geographic divergence. It seems that the biotype sharing the most similar biotypic profiles and the same geographic region with current prevailing one may have the greatest potential to become the new prevailing biotype. Close monitoring of greenbug population dynamics especially biotypic variation on both crop plants and noncultivated grasses in small grain production areas may be a useful strategy for detecting potentially new prevailing virulent biotypes of the greenbug.

  20. Seasonal phenology, spatial distribution, and sampling plan for the invasive mealybug Phenacoccus peruvianus (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Beltrá, A; Garcia-Marí, F; Soto, A

    2013-06-01

    Phlenacoccus peruvianus Granara de Willink (Hemiptera: Pseudococcidae) is an invasive mealybug of Neotropical origin. In recent years it has invaded the Mediterranean Basin causing significant damages in bougainvillea and other ornamental plants. This article examines its phenology, location on the plant and spatial distribution, and presents a sampling plan to determine P. peruvianus population density for the management of this mealybug in southern Europe. Six urban green spaces with bougainvillea plants were periodically surveyed between March 2008 and September 2010 in eastern Spain, sampling bracts, leaves, and twigs. Our results show that P. peruvianus abundance was high in spring and summer, declining to almost undetectable levels in autumn and winter. The mealybugs showed a preference for settling on bracts and there were no significant migrations between plant organs. P. peruvianus showed a highly aggregated distribution on bracts, leaves, and twigs. We recommend abinomial sampling of 200 leaves and an action threshold of 55% infested leaves for integrated pest management purposes on urban landscapes and enumerative sampling for ornamental nursery management and additional biological studies.

  1. Molecular Characterization and Expression Profiles of Polygalacturonase Genes in Apolygus lucorum (Hemiptera: Miridae.

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    Lili Zhang

    Full Text Available Polygalacturonase (PG is an enzyme in the salivary glands of piercing-sucking mirid bugs (Hemiptera: Miridae that plays a key role in plant feeding and injury. By constructing a full-length cDNA library, we cloned and characterized 14 PG genes from the salivary glands of Apolygus lucorum, a pestiferous mirid bug in cotton, fruit trees and other crops in China. BLAST search analysis showed that the amino acid sequences deduced from transcripts of the PG genes were closely related to PGs from other mirid bugs. Phylogenetic analysis showed that the PGs of mirid bugs had six main branches, PG1-PG6 (Genbank accession numbers: KF881899~KF881912. We investigated the mRNA expression patterns of the A. lucorum PG genes using real-time PCR. All 14 PGs were expressed significantly higher in the salivary glands than in other tissues (head, thorax, abdomen, leg and wing. For eggs and nymphs, the expression levels of these PGs were much higher in the 5th instar stage than in the egg, and 1st and 3rd instar stages. The PG expression levels in 1-day-old adults were very low, and increased in 5, 20 and 30-day-old adults. Additionally, PG expression levels were generally similar between males and females. The possible physiological functions of PGs in A. lucorum were discussed.

  2. Life table parameters of three Mirid Bug (Adelphocoris species (Hemiptera: Miridae under contrasted relative humidity regimes.

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    Hongsheng Pan

    Full Text Available The genus Adelphocoris (Hemiptera: Miridae is a group of important insect pests of Bt cotton in China. The three dominant species are A. lineolatus, A. suturalis, and A. fasciaticollis, and these species have different population dynamics. The causal factors for the differences in population dynamics have not been determined; one hypothesis is that humidity may be important for the growth of Adelphocoris populations. In the laboratory, the demographic parameters of the three Adelphocoris species were compared when the mirid bugs were subjected to various levels of relative humidity (40, 50, 60, 70 and 80% RH. Middle to high levels of RH (60, 70 and 80% were associated with higher egg and nymph survival rates and increased adult longevity and female fecundity. Lower humidity levels (40 and 50% RH had negative effects on the survival of nymphs, adult longevity and fecundity. The intrinsic rate of increase (rm, the net reproductive rate (R0 and the finite rate of increase (λ for each Adelphocoris species increased with increasing RH. Significant positive relationships were found between RH and the life table parameters, rm, R0 and λ for the three Adelphocoris species. These results will help to better understand the phenology of the three Adelphocoris species, and the information can be used in population growth models to optimize pest forecasting and management strategies for these key pests.

  3. Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae.

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    Nicoletta Faraone

    Full Text Available Plant-derived products can play an important role in pest management programs. Essential oils from Lavandula angustifolia (lavender and Thymus vulgaris (thyme and their main constituents, linalool and thymol, respectively, were evaluated for insecticidal activity and synergistic action in combination with insecticides against green peach aphid, Myzus persicae (Sulzer (Hemiptera: Aphididae. The essential oils and their main constituents exerted similar insecticidal activity when aphids were exposed by direct sprays, but were non-toxic by exposure to treated leaf discs. In synergism experiments, the toxicity of imidacloprid was synergized 16- to 20-fold by L. angustifolia and T. vulgaris essential oils, but far less synergism occurred with linalool and thymol, indicating that secondary constituents of the oils were probably responsible for the observed synergism. In contrast to results with imidacloprid, the insecticidal activity of spirotetramat was antagonized by L. angustifolia and T. vulgaris essential oils, and linalool and thymol. Our results demonstrate the potential of plant essential oils as synergists of insecticides, but show that antagonistic action against certain insecticides may occur.

  4. Characterization of resistance to adult spittlebugs (Hemiptera: Cercopidae) in Brachiaria spp.

    Science.gov (United States)

    Aguirre, Lina M; Cardona, César; Miles, John W; Sotelo, Guillermo

    2013-08-01

    Nymphs and adults of several spittlebug (Hemiptera: Cercopidae) species are key pests of forage brachiariagrasses (Brachiaria spp.) in tropical America. To support current breeding programs, a series of experiments aimed at characterizing the mechanisms of resistance to adult feeding damage were carried out. Five genotypes were used: two susceptible checks (CIAT 0606 and CIAT 0654) and three nymph-resistant genotypes (CIAT 36087, CIAT 6294, and SX01NO/0102). Test insects were Aeneolamia varia (F.), A. reducta (Lallemand), and Zulia carbonaria (Lallemand). The nymph-resistant genotypes showed tolerance to all spittlebug species tested. Tolerance in these genotypes can be classified as only moderate given the extent of losses (60-80%) caused by both female and male adults. None of the nymph-resistant genotypes had antibiotic effects on adults feeding on foliage. The results also indicated that antixenosis for feeding is not a plausible explanation for lower damage scores and less biomass losses in resistant genotypes. The fact that adult longevity (usually 8 d) was not affected when the adults were forced to feed on roots of a genotype with strong antibiotic resistance to nymphs is regarded as additional evidence that resistances to nymphs and to adults in Brachiaria are largely independent.

  5. Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)

    Science.gov (United States)

    Bardella, Vanessa Bellini; Pita, Sebastián; Vanzela, André Luis Laforga; Galvão, Cleber; Panzera, Francisco

    2016-01-01

    The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae. PMID:27759763

  6. A laboratory and field condition comparison of life table parameters of Aphis gossypii Glover (Hemiptera: Aphididae

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    Hosseini-Tabesh Behnaz

    2015-01-01

    Full Text Available Life table studies are essential tools for understanding population dynamics. The life table parameters of Aphis gossypii Glover (Hemiptera: Aphididae feeding on the host plant, Hibiscus syriacus L. were studied under laboratory (25±1°C and relative humidity of 65±5% and a photoperiod of 16L : 8D h and field conditions (23-43°C, and relative humidity of 27-95%. The data were analysed using the age-stage, two-sex life table theory. The life table studies were started with 50 and 40 nymphs in laboratory and field conditions, respectively. Under laboratory conditions, A. gossypii reared on H. syriacus had a higher survival rate, fecundity, and longevity than those reared under field conditions. When reared under field conditions, A. gossypii had a longer nymphal developmental time, shorter adult longevity, and lower fecundity than those reared under laboratory conditions. The intrinsic rate of increase (r, net reproductive rate (R0, and the finite rate of increase (λ under laboratory conditions, were higher than those obtained under field conditions. Nevertheless, there were no significant differences in the mean generation time T (days between field and laboratory conditions. In the present study, the results clearly showed that life table parameters of A. gossypii were significantly different under field and laboratory conditions. These results could help us to understand the A. gossypii population dynamics under field conditions. The results could also help us make better management decisions for economically important crops

  7. Within-plant distribution and binomial sampling of Pentalonia nigronervosa (Hemiptera: Aphididae) on banana.

    Science.gov (United States)

    Robson, Jacqueline D; Wright, Mark G; Almeida, Rodrigo P P

    2006-12-01

    The banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), infests banana (Musa spp.) worldwide. Pentalonia nigronervosa is the vector of Banana bunchy top virus (family Nanoviridae, genus Babuvirus) the etiological agent of Banana bunchy top disease (BBTD). BBTD is currently the most serious problem affecting banana in Hawaii. Despite the importance of this vector species, little is known about its biology or ecology. There are also no sampling plans available for P. nigronervosa. We conducted field surveys to develop a sampling plan for this pest. Ten plots were surveyed on seven commercial banana farms on the island of Oahu, HI, for the presence of P. nigronervosa on banana plantlets. We found aphids more frequently near the base of plants, followed by the newest unfurled leaf at the top of the plant. Aphids were least likely to be located on leaves in between the top and bottom of the plant. Aphid infestation on surveyed plots ranged from 8 to 95%. We developed a sequential binomial sampling plan based on our surveys. We also discovered that the within-plant distribution of P. nigronervosa is an important factor to consider when sampling for this pest. Our sampling plan will assist in the development of sustainable management practices for banana production.

  8. Life History of the Camelthorn Gall Leafhopper, Scenergates viridis (Vilbaste (Hemiptera, Cicadellidae

    Directory of Open Access Journals (Sweden)

    Roman Rakitov

    2012-01-01

    Full Text Available The world’s only member of Hemiptera Auchenorrhyncha known to form true galls, the leafhopper Scenergates viridis (Vilbaste (Cicadellidae, transforms leaves of camelthorn (Alhagi maurorum Medikus, Fabaceae into pod-like chambers, up to 35 mm long, inside which individual leafhoppers develop, mate, and lay eggs. At the study site 40 km SE of Bukhara (Uzbekistan, two generations develop annually. First-instar nymphs cause young leaves to fold along the midrib. The subsequent development takes place inside the tightly closed growing gall, plugged at both ends with a mixture of leafhopper excrement, brochosomes, and crushed exuviae. These plugs act as mechanical barriers and sticky traps for intruders. The inner surface of the gall, lined with brochosomes and wax platelets, is hydrophobic. Adult males emerge from their galls and squeeze into female galls. Fertilized females insert an average of 146 eggs under the gall’s inner epidermis and remain inside, possibly protecting the brood, until they die. The walls of the galls containing eggs are approximately three times thicker than regular leaves. The galls are subject to predation by Gelechiidae caterpillars; the eggs of the leafhopper are parasitized by two species of Trichogrammatidae and one Mymaridae (Hymenoptera, and its larvae by one species of Pipunculidae (Diptera.

  9. The foraging behavior of Diaeretiella rapae (Hymenoptera: Braconidae on Diuraphis noxia (Hemiptera: Aphididae

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    Tazerouni Zahra

    2011-01-01

    Full Text Available Host stage preference, functional response and mutual interference of Diaeretiella rapae (McIntosh (Hymenoptera: Braconidae: Aphidiinae on Diuraphis noxia (Mordvilko (Hemiptera: Aphididae were investigated under defined laboratory conditions (20±1°C; 60±5% relative humidity; 16 h light/8 h dark photoperiod. Nicholson’s model and linear regression were used to determine per capita search-efficiency and the interference coefficient, respectively. There was a significant difference between the rates of parasitism on different stages of D. noxia. The highest parasitism percentage was observed on the third instar nymphs of D. noxia in both choice and no-choice preference tests. Results of logistic regression revealed a type II functional response. The estimated values of search-efficiency (a and handling time (Th were 0.072 h-1 and 0.723 h, respectively. The maximum attack rate was calculated to be 33.22. The per capita search-efficiency decreased from 0.011 to 0.004 (h-1 as parasitoid densities increased from 1 to 8. Therefore, different host-parasitoid ratios can affect the efficacy of D. rapae.

  10. Impact of Feeding on Contaminated Prey on the Life Parameters of Nesidiocoris Tenuis (Hemiptera: Miridae) Adults

    Science.gov (United States)

    Wanumen, Andrea Carolina; Sánchez-Ramos, Ismael; Viñuela, Elisa; Medina, Pilar; Adán, Ángeles

    2016-01-01

    Insecticide selectivity to natural enemies is an important concern in integrated pest management programs. Although there is a wide range of information concerning pesticide lethal and sublethal effects on contaminated surfaces, little is known when the route of exposure occurs at a trophic level. This study evaluates this route of pesticide intake on the omnivorous predator Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae) for the first time. Under laboratory conditions, prey treated with six insecticides (flubendiamide, spirotetramat, deltamethrin, flonicamid, metaflumizone, and sulfoxaflor) were offered to N. tenuis adults for 3 days. Mortality (24, 48, and 72 h after treatment), offspring production (third until eighth day) and longevity were documented. Metaflumizone and sulfoxaflor were classified as moderately harmful products because although the percentage of mortality was only 28 and 36%, respectively, both products caused a severe decrease in offspring production and longevity. Flonicamid and flubendiamide were classified as slightly harmful products; although they did not have a lethal effect, sublethal impact was important on the parameters studied. Spirotetramat and deltamethrin were insecticides categorized as harmless. This information could be useful for selecting the most appropriate insecticides to control pests in tomato crops in which N. tenuis is a relevant biological control agent. PMID:27694345

  11. Solenopsis invicta (Hymenoptera: Formicidae), defend Phenacoccus solenopsis (Hemiptera: Pseudococcidae) against its natural enemies.

    Science.gov (United States)

    Zhou, Aiming; Lu, Yongyue; Zeng, Ling; Xu, Yijuan; Liang, Guangwen

    2013-04-01

    Mutualism is a common and important ecological phenomenon characterized by beneficial interaction between two species. Red imported fire ants, Solenopsis invicta Buren, tend honeydew-producing hemipteran insects and reduce the activity of these insects' enemies. Ant-hemipteran interactions frequently exert positive effects on the densities of hemipterans. We tested the hypothesis that ant tending can increase the densities of the mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), and reduce the densities of the mealybug's predatory and parasitic enemies, the lady beetle, Menochilus sexmaculata Fabricius (Coleoptera: Coccinellidae), and the parasitoid wasp, Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae). We found that more ants foraged on mealybug-infested hibiscus plants than on mealybug-free plants. The number of foraging ants on plants infested with high densities of mealybugs (62.5 ants per plant) was nearly six times that on mealybug-free plants (10.2 ants per plant). Experiment results showed that ant tending significantly increased the survival of mealybugs: if predatory and parasitic enemies were present, the survival of mealybugs tended by fire ants was higher than that in the absence of tending ants. Furthermore, this tending by fire ants significantly decreased the survival of lady beetle larvae. However, no apparent effect was observed on the survival of parasitoid.

  12. Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae in Brazilian vineyards.

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    Vitor C Pacheco da Silva

    Full Text Available Mealybugs (Hemiptera: Pseudococcidae are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell, Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley, Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso, Pseudococcus viburni (Signoret, Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret. Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.

  13. Development and Life Table Parameters of Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae) on Four Ornamental Plants.

    Science.gov (United States)

    Tok, B; Kaydan, M B; Mustu, M; Ulusoy, M R

    2016-08-01

    The development, reproduction, and life table parameters of the Phenacoccus madeirensis Green (Hemiptera: Pseudococcidae) on four ornamental plant species, namely Pelargonium zonale (Geraniaceae), Hibiscus rosa-sinensis, Hibicus syriacus (Malvaceae), and Cestrum nocturnum (Solanaceae) were investigated under controlled conditions (25 ± 2°C, 60 ± 10% R.H., and 16 h photophase). Life table data were analyzed by using an age-stage two-sex life table. The shortest total immature developmental time of females and males for P. madeirensis was obtained on C. nocturnum (20.42 and 21.90 days, respectively). The highest fecundities were 233 and 232 eggs on C. nocturnum and H. syriacus, respectively. The intrinsic rate of increase (r  = 0.1511 day(-1)) and finite rate of increase (λ  =  1.1631 day(-1)) were the greatest when mealybugs were reared on C. nocturnum. Net reproductive rate (R 0  =  129.5 offspring) was the greatest when reared on H. syriacus, but this value was not statistically different from that on C. nocturnum. The shortest mean generation time (T  =  31.3 days) was calculated on C. nocturnum. These results indicate that C. nocturnum and H. syriacus are more suitable hosts than H. rosa-sinensis and P. zonale for P. madeirensis.

  14. Citrus mealybug (Hemiptera: Pseudococcidae) movement and population dynamics in an arbor-trained vineyard.

    Science.gov (United States)

    Cid, M; Pereiro, S; Cabaleiro, C; Segura, A

    2010-06-01

    The citrus mealybug, Planococcus citri (Risso) (Hemiptera: Pseudococcidae), is the main grapevine pest in vineyards in some countries, such as Spain and Brazil. In Galician vineyards (northwestern Spain), mealybug population levels are low because the accumulated degree-days are lower than in other grapevine-growing areas. The main problem caused by mealybugs is the transmission of viruses, even at low infestation levels. The active period of citrus mealybug in the study vineyard lasted from July until December, with an important movement peak at the end of July and August and a lower peak in November. The mealybug mainly moved upward along arbor-trained plants, and there were no important downward movements at the end of the season as has been described for other grapevine mealybugs. The mealybugs were normally restricted to the woody organs and were only present on leaves, branches, and green canes (always close to woody parts) in plants with high infestations. The movement of mealybugs between plants does not seem to take place by contact between green organs. Passive aerial transport and movement of pruning remains may play an important role in mealybug movement and thus in spread of the virus. The number of mealybugs carrying Grapevine leafroll-associated virus 3 (GLRaV-3) was found to represent approximately 75% of mealybugs caught in a GLRaV-3 infected vineyard.

  15. Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in Brazilian vineyards.

    Science.gov (United States)

    Pacheco da Silva, Vitor C; Bertin, Aline; Blin, Aurélie; Germain, Jean-François; Bernardi, Daniel; Rignol, Guylène; Botton, Marcos; Malausa, Thibaut

    2014-01-01

    Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell), Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley), Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso), Pseudococcus viburni (Signoret), Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn) and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species.

  16. A multiplex PCR assay for the simultaneous identification of three mealybug species (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Saccaggi, D L; Krüger, K; Pietersen, G

    2008-02-01

    Molecular species identification is becoming more wide-spread in diagnostics and ecological studies, particularly with regard to insects for which morphological identification is difficult or time-consuming. In this study, we describe the development and application of a single-step multiplex PCR for the identification of three mealybug species (Hemiptera: Pseudococcidae) associated with grapevine in South Africa: Planococcus ficus (vine mealybug), Planococcus citri (citrus mealybug) and Pseudococcus longispinus (longtailed mealybug). Mealybugs are pests on many commercial crops, including grapevine, in which they transmit viral diseases. Morphological identification of mealybug species is usually time-consuming, requires a high level of taxonomic expertise and usually only adult females can be identified. The single-step multiplex PCR developed here, based on the mitochondrial cytochrome c oxidase subunit 1 (CO I) gene, is rapid, reliable, sensitive, accurate and simple. The entire identification protocol (including DNA extraction, PCR and electrophoresis) can be completed in approximately four hours. Successful DNA extraction from laboratory and unparasitized field-collected individuals stored in absolute ethanol was 97%. Specimens from which DNA could be extracted were always correctly identified (100% accuracy). The technique developed is simple enough to be implemented in any molecular laboratory. The principles described here can be extended to any organism for which rapid, reliable identification is needed.

  17. Phenotypic variation and identification of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) in China.

    Science.gov (United States)

    Zhao, Jing; Watson, Gillian W; Sun, Yang; Tan, Yongan; Xiao, Liubin; Bai, Lixin

    2014-05-23

    Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is an invasive mealybug that seriously damages cotton and other important crops. In previous studies in China, the presence of two submedian longitudinal lines of pigmented spots on the dorsum of adult females frequently has been used to identify this species. However, the present study records the occasional absence of pigmented spots in a sample from Guangxi province, China. Specimens without pigmented spots showed all the molecular and morphological characters that separate P. solenopsis from the similar species P. solani Ferris, especially the distribution of multilocular disc pores. In different geographic populations of P. solenopsis in China, mitochondrial COI and nuclear 28SrDNA genes are very similar (99.8-100%), indicating that they are conspecific. For COI, the genetic distance between P. solenopsis and P. solani is more than 3%. A map of the distribution of P. solenopsis in China is given. To help identify both pigmented and non-pigmented P. solenopsis accurately, an identification key to the 16 species of Phenacoccus found in China is provided. The key also identifies five potentially invasive Phenacoccus species not yet established in China, in case they get introduced there.

  18. Ultralow oxygen treatment for control of Planococcus ficus (Hemiptera: Pseudococcidae) on grape benchgrafts.

    Science.gov (United States)

    Liu, Yong-Biao; Bettiga, Larry J; Daane, Kent M

    2010-04-01

    Controlled atmosphere treatments with ultralow oxygen (ULO treatments) were developed successfully for control of vine mealybug, Planococcusflcus Signoret (Hemiptera: Pseudococcidae), on dormant grape (Vitis spp.) benchgrafts. At 30 ppm oxygen, 3-d ULO treatment at 25 degrees C and 4-d ULO treatment at 150C achieved complete control of all life stages of P. ficus. At a much lower oxygen level (<1 ppm), the two ULO treatments with the same exposure periods of 3 d at 25 degrees C and 4 d at 15 degrees C were tested on six table and wine grape cultivars grafted on rootstocks along with P. ficus. The benchgrafts were then potted in a greenhouse, together with untreated controls, to determine treatment effects on rootstock viability. Both ULO treatments achieved complete control of P. ficus and did not have any negative effects on vine growth, compared with the control. Results indicate that ULO treatments can be used to control P. ficus on dormant grape benchgrafts. The advantages of the ULO treatments are also discussed with respect to hot water treatments.

  19. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Fatima, Samman; Hussain, Mubashar; Shafqat, Shama; Faheem Malik, Muhammad; Abbas, Zaheer; Noureen, Nadia; Ul Ane, Noor

    2016-01-01

    Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae), is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14%) of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83%) was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  20. Species clarification of Isaria isolates used as biocontrol agents against Diaphorina citri (Hemiptera: Liviidae) in Mexico.

    Science.gov (United States)

    Gallou, Adrien; Serna-Domínguez, María G; Berlanga-Padilla, Angélica M; Ayala-Zermeño, Miguel A; Mellín-Rosas, Marco A; Montesinos-Matías, Roberto; Arredondo-Bernal, Hugo C

    2016-03-01

    Entomopathogenic fungi belonging to the genus Isaria (Hypocreales: Cordycipitaceae) are promising candidates for microbial control of insect pests. Currently, the Mexican government is developing a biological control program based on extensive application of Isaria isolates against Diaphorina citri (Hemiptera: Liviidae), a vector of citrus huanglongbing disease. Previous research identified three promising Isaria isolates (CHE-CNRCB 303, 305, and 307; tentatively identified as Isaria fumosorosea) from Mexico. The goal of this work was to obtain a complete morphological and molecular characterization of these isolates. Comparative analysis of morphology established that the isolates showed similar characteristics to Isaria javanica. Multi-gene analysis confirmed the morphological identification by including the three isolates within the I. javanica clade. Additionally, this work demonstrated the misidentifications of three other Isaria isolates (CHE-CNRCB 310 and 324: I. javanica, formerly I. fumosorosea; CHE-CNRCB 393: I. fumosorosea, formerly Isaria farinosa), underlying the need for a full and correct characterization of an isolate before developing a biological control program. Finally, the inter-simple sequence repeat (ISSR) genotyping method revealed that the CHE-CNRCB 303, 305, and 307 isolates belong to three different genotypes. This result indicates that ISSR markers could be used as a tool to monitor their presence in field conditions.

  1. Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae).

    Science.gov (United States)

    Faraone, Nicoletta; Hillier, N Kirk; Cutler, G Christopher

    2015-01-01

    Plant-derived products can play an important role in pest management programs. Essential oils from Lavandula angustifolia (lavender) and Thymus vulgaris (thyme) and their main constituents, linalool and thymol, respectively, were evaluated for insecticidal activity and synergistic action in combination with insecticides against green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). The essential oils and their main constituents exerted similar insecticidal activity when aphids were exposed by direct sprays, but were non-toxic by exposure to treated leaf discs. In synergism experiments, the toxicity of imidacloprid was synergized 16- to 20-fold by L. angustifolia and T. vulgaris essential oils, but far less synergism occurred with linalool and thymol, indicating that secondary constituents of the oils were probably responsible for the observed synergism. In contrast to results with imidacloprid, the insecticidal activity of spirotetramat was antagonized by L. angustifolia and T. vulgaris essential oils, and linalool and thymol. Our results demonstrate the potential of plant essential oils as synergists of insecticides, but show that antagonistic action against certain insecticides may occur.

  2. Fitness of Encarsia sophia(Hymenoptera: Aphelinidae)parasitizing Trialeurodes vaporariorum and Bemisia tabaci (Hemiptera: Aleyrodidae)

    Institute of Scientific and Technical Information of China (English)

    Chen Luo; Tong-Xian Liu

    2011-01-01

    Fitness and efficacy of Encarsia sophia(Girault & Dodd)(Hymenoptera:Aphelinidae)as a biological control agent was compared on two species of whitefly(Hemiptera: Aleyrodidae)hosts,the relatively smaller sweetpotato whitefly,Bemisia tabaci(Gennadius)biotype 'B',and the larger greenhouse whitefly,Trialeurodes vaporariorum(Westwood).Significant differences were observed on green bean(Phaseolus vulgaris L.)in the laboratory at 27± 2℃,55% ± 5% RH,and a photoperiod of 14 : 10 h(L : D).Adult parasitoids emerging from T.vaporariorum were larger than those emerging from B.tabaci,and almost all biological parameters of E.sophia parasitizing the larger host species were superior except for the developmental times of the parasitoids that were similar when parasitizing the two host species.Furthermore,parasitoids emerging from T.vaporariorum parasitized more of these hosts than did parasitoids emerging from B.tabaci.We conclude that E.sophia reared from larger hosts had better fitness than from smaller hosts.Those from either host also preferred the larger host for oviposition but were just as effective on smaller hosts.Therefore,larger hosts tended to produce better parasitoids than smaller hosts.

  3. [Characterization of the behavioral and vibrational signals in Euthyrhynchus floridanus (Hemiptera: Pentatomidae) during courtship and copulation].

    Science.gov (United States)

    Briceño, R Daniel

    2014-03-01

    Euthyrhynchus floridanus (Linnaeus) (Hemiptera: Pentatomidae) is a neotropical species belonging to the family Pentatomidae with over 4 000 species described, and is distributed from Florida to Brazil. This study describes the sexual behavior and reported for the first time the production of substrate vibrations by males and females during copulatory behavior and mating. Courtship and copulatory behavior, as well as the diverse signals, were recorded with a phonographic cartridge connected to a video camera. Female vibrations were reproduced in the absence of females and the responses by males were recorded. At least three types of substrate vibrations were distinguished in males and one in females, and these signals were characterized by their low frequency, varying from 127 to 180Hz. The sounds of E. floridianus males were significantly different in frequency, duration and number of pulses, both in courtship and in copulation, for the purring and drumming sounds. The production of sounds in this species is associated principally with mechanical, stimulatory behavior during courtship and copulation. Patterns of behavior and their relation to substrate vibrations suggest that these signals are important for the males in the context of mate location and sexual selection.

  4. Effect of vermicompost and cucumber cultivar on population growth attributes of the melon aphid (Hemiptera: Aphididae).

    Science.gov (United States)

    Razmjou, J; Mohammadi, M; Hassanpour, M

    2011-08-01

    Worldwide, the developing industry of cucumbers (Cucumis sativus L.) grown in greenhouses is threatened by damage from sucking pests, especially aphids. Among these, the melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is the most serious. We tested the effect of two cucumber cultivars ('Royal' and'Storm') and three vermicompost concentrations (0 [control], 20, and 30%) in field soil on the development and fecundity rates of A. gossypii, by using a randomized complete block design with four replicates as a factorial experiment. The developmental times of nymphs reared on plants grown into the three vermicompost concentrations ranged from 5.5 (0%) to 8.7 (30%) d (on Storm) and from 4.3 (0%) to 7 (30%) d (on Royal). The developmental time of melon aphid's nymphs was greatest on plants grown in the culture medium with 30% vermicompost rate and least on plants reared in the soil without vermicompost. The intrinsic rate of natural increase (r,,) of A. gossypii ranged from 0.204 d(-1) on plants grown in the soil amended with 30% vermicompost rate (on Storm seedlings) to 0.458 d(-1) on plants grown in the soil without vermicompost (on Royal seedlings). Accordingly, our findings confirm that a combination of a low level of vermicompost and a partially resistant cucumber cultivar might play an important role in managing this aphid on cucumbers in greenhouses.

  5. Gammaproteobacteria as essential primary symbionts in the striped shield bug, Graphosoma Lineatum (Hemiptera: Pentatomidae)

    Science.gov (United States)

    Karamipour, Naeime; Mehrabadi, Mohammad; Fathipour, Yaghoub

    2016-01-01

    Many members of suborder Heteroptra harbor heritable symbiotic bacteria. Here we characterize the gut symbiotic bacterium in Graphosoma lineatum (Hemiptera: Pentatomidae) by using molecular phylogeny, real-time PCR analysis as well as light and electron microscopy observations. The microscopy observations revealed the presence of a large number of rod-shaped bacterial cells in the crypts. A very high prevalence (98 to 100%) of the symbiont infection was found in the insect populations that strongly supports an intimate association between these two organisms. Real-time PCR analysis also showed that the Gammaproteobacteria dominated the crypts. The sequences of 16sr RNA and groEL genes of symbiont showed high levels of similarity (93 to 95%) to Pantoea agglomeranse and Erwinia herbicola Gammaproteobacteria. Phylogenetic analyses placed G. lineatum symbiont in a well-defined branch, divergent from other stink bug bacterial symbionts. Co-evolutionary analysis showed lack of host-symbiont phylogenetic congruence. Surface sterilization of eggs resulted in increased pre-adult stage in the offspring (aposymbionts) in comparison to the normal. Also, fecundity, longevity, and adult stage were significantly decreased in the aposymbionts. Therefore, it seems that the symbiont might play a vital function in the host biology, in which host optimal development depends on the symbiont. PMID:27609055

  6. The hemiptera (insecta of Canada: constructing a reference library of DNA barcodes.

    Directory of Open Access Journals (Sweden)

    Rodger A Gwiazdowski

    Full Text Available DNA barcode reference libraries linked to voucher specimens create new opportunities for high-throughput identification and taxonomic re-evaluations. This study provides a DNA barcode library for about 45% of the recognized species of Canadian Hemiptera, and the publically available R workflow used for its generation. The current library is based on the analysis of 20,851 specimens including 1849 species belonging to 628 genera and 64 families. These individuals were assigned to 1867 Barcode Index Numbers (BINs, sequence clusters that often coincide with species recognized through prior taxonomy. Museum collections were a key source for identified specimens, but we also employed high-throughput collection methods that generated large numbers of unidentified specimens. Many of these specimens represented novel BINs that were subsequently identified by taxonomists, adding barcode coverage for additional species. Our analyses based on both approaches includes 94 species not listed in the most recent Canadian checklist, representing a potential 3% increase in the fauna. We discuss the development of our workflow in the context of prior DNA barcode library construction projects, emphasizing the importance of delineating a set of reference specimens to aid investigations in cases of nomenclatural and DNA barcode discordance. The identification for each specimen in the reference set can be annotated on the Barcode of Life Data System (BOLD, allowing experts to highlight questionable identifications; annotations can be added by any registered user of BOLD, and instructions for this are provided.

  7. A preliminary molecular phylogeny of planthoppers (Hemiptera: Fulgoroidea based on nuclear and mitochondrial DNA sequences.

    Directory of Open Access Journals (Sweden)

    Nan Song

    Full Text Available The planthopper superfamily Fulgoroidea (Insecta: Hemiptera is one of the most dominant groups of phytophagous insects. It comprises about 20 families, containing a total of 9000 species worldwide. Despite several recent studies, the phylogeny of Fulgoroidea is not yet satisfactorily resolved and the phylogenetic positions of several key families, especially Cixiidae, Delphacidae, Tettigometridae, Nogodinidae, Acanaloniidae and Issidae, are contentious. Here, we expand upon recent phylogenetic work using additional nuclear (18S and 28S and novel mitochondrial (16S and cytb markers. Maximum likelihood and Bayesian analyses yielded robust phylogenetic trees. In these topologies, a group containing Cixiidae and Delphacidae is recovered as the sister group to the remaining taxa. Tettigometridae is placed in a more nested position and is grouped with Caliscelidae. Sister relationships are found between Flatidae and Ricaniidae, and between Dictyopharidae and Fulgoridae. Nogodinidae and Issidae are confirmed to be non-monophyletic families. For major nodes of interest, divergence date estimates are generally older than those from the fossil record.

  8. The Spread of Nezara viridula (Hemiptera:Pentatomidae Species from its First Occurrence in Romania

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    Ioana Grozea

    2016-11-01

    Full Text Available Lately in horticultural agroecosystems from Romania joined several insect species autochthonous. Among these is a species of bugs known as southern green stink bugs or Nezara viridula L. (Insecta: Hemiptera: Pentatomidae. Unlike other new entrants this bug species has a diversified polifagism being present in many plants but obvious damage produce only tomato fruit. The causes are unknown. Can be found in gardens, green spaces and parks. Often is observed in vegetable gardens where populations comprise all stages (egg, larva/nymph, adult. Although it has African origins of a warm area is interesting installation and survival in temperate zones like those in Europe. Presence in Europe appears to be more random which excludes primarily spread through the neighborhood. By observations made in west of Romania, during 2010-2015 we wanted to watch the evolution of the insect from the first point of occurrence (Timișoara, 2010. Also, were monitored tomato crops and ornamentals in gardens and green spaces from 5 counties. Only in four monitored counties it was observed this species. Most adults and larvae were registered in Timis county (5-6 adults or 7-8 larvae/tomato plant and 10 -11 adults or 15-20 larvae/ornamental shrub. In our country the insect has proven to be capable of a rapid spread in a relatively short time interval including surrounding counties.

  9. Spatial Distribution of Adults of Triozoida limbata (Enderlein) (Hemiptera: Triozidae) in Guava Plants.

    Science.gov (United States)

    Marcelino, M C S; Barbosa, J C

    2016-04-01

    The psyllid Triozoida limbata (Enderlein) (Hemiptera: Triozidae) is a major pest in guava, feeding primarily on new shoots. Despite its importance, there are no studies on the spatial distribution of T. limbata on guava. Such studies are needed to establish sequential sampling plans for decision making in pest control. Thus, an experiment was carried out in a 9-year-old commercial guava orchard divided into 100 sampling units or plots. Double-sided yellow sticky traps were placed on one plant per plot (sample unit) to capture and monitor T. limbata adults from April 2011 to May 2012. To determine the insect distribution in the area, we calculated the variance-to-mean ratio index (I), the Morisita index (I δ ), Green's coefficient (Cx), and the k exponent of the negative binomial distribution. Most of the samples showed that the adults had a moderate to highly aggregated distribution. Statistical models were also used to study the pest spatial distribution by fitting the number of adults captured to the Poisson and negative binomial distributions. The negative binomial distribution model best fitted the data of the number of adult psyllids captured by the traps, which is consistent with an aggregated distribution.

  10. Characterization of a newly discovered symbiont of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Bing, Xiao-Li; Yang, Jiao; Zchori-Fein, Einat; Wang, Xiao-Wei; Liu, Shu-Sheng

    2013-01-01

    Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 cryptic species, some of which are important crop pests worldwide. Like many other sap-sucking insects, whiteflies harbor an obligatory symbiont, "Candidatus Portiera aleyrodidarum," and a number of secondary symbionts. So far, six genera of secondary symbionts have been identified in B. tabaci. In this study, we report and describe the finding of an additional bacterium in the indigenous B. tabaci cryptic species China 1 (formerly known as B. tabaci biotype ZHJ3). Phylogenetic analysis based on the 16S rRNA and gltA genes showed that the bacterium belongs to the Alphaproteobacteria subdivision of the Proteobacteria and has a close relationship with human pathogens of the genus Orientia. Consequently, we temporarily named it Orientia-like organism (OLO). OLO was found in six of eight wild populations of B. tabaci China 1, with the infection rate ranging from 46.2% to 76.8%. Fluorescence in situ hybridization (FISH) of B. tabaci China 1 in nymphs and adults revealed that OLOs are confined to the bacteriome and co-occur with "Ca. Portiera aleyrodidarum." The vertical transmission of OLO was demonstrated by detection of OLO at the anterior pole end of the oocytes through FISH. Quantitative PCR analysis of population dynamics suggested a complex interaction between "Ca. Portiera aleyrodidarum" and OLO. Based on these results, we propose "Candidatus Hemipteriphilus asiaticus" for the classification of this symbiont from B. tabaci.

  11. Predictive value of Sp1/Sp3/FLIP signature for prostate cancer recurrence.

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    Roble G Bedolla

    Full Text Available Prediction of prostate cancer prognosis is challenging and predictive biomarkers of recurrence remain elusive. Although prostate specific antigen (PSA has high sensitivity (90% at a PSA level of 4.0 ng/mL, its low specificity leads to many false positive results and considerable overtreatment of patients and its performance at lower ranges is poor. Given the histopathological and molecular heterogeneity of prostate cancer, we propose that a panel of markers will be a better tool than a single marker. We tested a panel of markers composed of the anti-apoptotic protein FLIP and its transcriptional regulators Sp1 and Sp3 using prostate tissues from 64 patients with recurrent and non-recurrent cancer who underwent radical prostatectomy as primary treatment for prostate cancer and were followed with PSA measurements for at least 5 years. Immunohistochemical staining for Sp1, Sp3, and FLIP was performed on these tissues and scored based on the proportion and intensity of staining. The predictive value of the FLIP/Sp1/Sp3 signature for clinical outcome (recurrence vs. non-recurrence was explored with logistic regression, and combinations of FLIP/Sp1/Sp3 and Gleason score were analyzed with a stepwise (backward and forward logistic model. The discrimination of the markers was identified by sensitivity-specificity analysis and the diagnostic value of FLIP/Sp1/Sp3 was determined using area under the curve (AUC for receiver operator characteristic curves. The AUCs for FLIP, Sp1, Sp3, and Gleason score for predicting PSA failure and non-failure were 0.71, 0.66, 0.68, and 0.76, respectively. However, this increased to 0.93 when combined. Thus, the "biomarker signature" of FLIP/Sp1/Sp3 combined with Gleason score predicted disease recurrence and stratified patients who are likely to benefit from more aggressive treatment.

  12. A revision of the Chinese species of Cyamophiliopsis (Hemiptera: Psylloidea: Psyllidae) associated with Spiraea (Rosaceae).

    Science.gov (United States)

    Luo, Xinyu; Li, Fasheng; Cai, Wanzhi

    2015-03-20

    Cyamophiliopsis is a small genus restricted to the Palaearctic Region and associated with Spiraea spp. (Rosaceae). In the present work, following five species are recognized in China: C. pseudofasciata sp. nov., C. sarmatica, C. spinosa sp. nov., C. xinjiangana sp. nov. and C. zaisani. Cyamophiliopsis is diagnosed, redescribed in detail, and its phylogenetic relationships are discussed. All the species are described or redescribed, and the fifth instar immature of C. zaisani is described for the first time. Nomenclatorial problems are discussed concerning the Far East Russian Psylla spiraee which is transferred to Cyamophiliopsis as C. spiraee comb. nov.

  13. Estados de desarrollo y biología de tres especies de Lygaeinae (Hemiptera-Heteroptera: Lygaeoidea: Lygaeidae Life stages and biology of three species of Lygaeinae (Hemiptera-Heteroptera: Lygaeoidea: Lygaeidae

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    Luis Cervantes-Peredo

    2007-12-01

    Full Text Available Se describen los estados de desarrollo (huevo, ninfas y adulto de 3 especies de Lygaeinae (Hemiptera-Heteroptera: Lygaeoidea: Lygaeidae, Anochrostomus formosus (Blanchard principalmente asociada con especies de Asteraceae y Convolvulaceae, y Lygaeus reclivatus reclivatus(Say y Oncopeltus (Oncopeltus sexmaculatus (Stål asociadas con Asclepiadaceae. Las descripciones están basadas en ejemplares colectados en los estados de Oaxaca y Guerrero (México y criados en el laboratorio. Se ilustra cada uno de los estadios y se incluyen notas acerca de su biología y plantas huéspedes.The life stages (egg, nymphs, and adult of 3 species of Lygaeinae (Hemiptera-Heteroptera: Lygaeoidea: Lygaeidae are described. Anochrostomus formosus (Blanchard is mainly associated with species of Asteraceae and Convolvulaceae, whereas Lygaeus reclivatus reclivatus (Say and Oncopeltus (Oncopeltus sexmaculatus (Stål are associated with Asclepiadaceae. Descriptions are based on individuals collected in the states of Oaxaca and Guerrero (Mexico, and reared in laboratory. Illustrations of each instar are also included, as well as notes about their biology and host plants.

  14. Nuevos registros de Hesperolabops nigriceps Reuter (Hemiptera: Miridae en el oriente del Estado de México New records of Hesperolabops nigriceps Reuter (Hemiptera: Miridae in the east of Mexico State

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    Marina Ruiz-Machuca

    Full Text Available El nopal tunero (Opuntia spp. se cultiva en todo el país, pero sólo en el Estado de México se concentra cerca de 35% de la producción nacional de tuna. Opuntia ficus-indica L. (Miller, es la especie predominante en la obtención de tunas, pero se carece de información que identifiquen taxonómicamente algunas plagas del cultivo; tal es el caso de la chinche roja (Hemiptera: Miridae. se realizaron colectas sistemáticas de este insecto sobre nopales (Opuntia spp., en diferentes municipios del Estado de México en 2008 y se encontraron 20 nuevos registros de Hesperolabops nigriceps Reuter.The prickly pear nopal (Opuntia spp. is cultivated throughout entire country, but 35% of the national production is found only within State of Mexico. Opuntia ficus-indica L. (Miller is the predominant species for obtaining prickly pears, but there is not any information available that would taxonomically identify some plagues particular to this crop; such is the case of red bug (Hemiptera: Miridae. Some of these insects were gathered from prickly pear nopal (Opuntia spp. in different municipalities of State of Mexico in 2008 and 20 new cases of Hesperolabops nigriceps Reuter were found.

  15. Illustrated checklist of mileewine leafhoppers (Hemiptera: Cicadellidae: Mileewinae) of China, with descriptions of four new species.

    Science.gov (United States)

    Yang, Mao-Fa; Meng, Ze-Hong; He, Qing; Dietrich, C H

    2014-01-01

    Four new species of Mileewini, Mileewa houhensis Yang, Meng & He, sp. nov., M. xiaofeiae Yang, Meng & He, sp. nov., M. yangi Yang, Meng & He, sp. nov. and Ujna liangae Yang, Meng & He, sp. nov. are described and illustrated. Mileewa nigricauda Yang & Li, 1999 is placed as a junior synonym of M. dorsimaculata (Melichar, 1902); three of the previously described Chinese Mileewa are transferred to the genus Ujna. These are Ujna harpa (Yang & Li, 2004) comb. nov., U. nigrimaculata (Yang & Li, 2004) comb. nov., and U. puerana (Yang & Meng, 2010) comb. nov. M. lynchi (Distant, 1918) is recorded as new for China. A checklist of Mileewini leafhoppers of China along with photograph of their dorsal habitus is provided.

  16. A new species of Coccus (Hemiptera, Coccoidea, Coccidae from China

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    Fang Wang

    2012-11-01

    Full Text Available A new species of soft scale, Coccus multisetus Wang & Feng, sp. n. is described and illustrated from Yunnan, China. A key to adult females of all Coccus known from China is provided.

  17. IIem-spFRET: improved Iem-spFRET method for robust FRET measurement

    Science.gov (United States)

    Zhang, Jiang; Lin, Fangrui; Chai, Liuying; Wei, Lichun; Chen, Tongsheng

    2016-10-01

    We recently developed a quantitative Förster resonance energy transfer (FRET) measurement method based on emission-spectral unmixing (Iem-spFRET). We here developed an improved Iem-spFRET method (termed as IIem-spFRET) for more robust FRET measurement in living cells. First, two background (BG) spectral fingerprints measured from blank living cells are introduced to remove BG and autofluorescence. Second, we introduce a ρ factor denoting the ratio of two molar extinction coefficient ratios (γ) of acceptor to donor at two excitations into IIem-spFRET for direct measurement of the γ values using a tandem construct with unknown FRET efficiency (E). We performed IIem-spFRET on our microscope-spectrometer platform to measure the γ values of Venus (V) to Cerulean (C) and the E values of C32V, CVC, VCV, and VCVV constructs, respectively, in living Huh7 cells. For the C32V or CVC cells, the Iem-spFRET and IIem-spFRET methods measured consistent E values. However, for the cells especially with low expressing levels of VCV or VCVV, the E values measured by Iem-spFRET showed large deviations and fluctuations, whereas the IIem-spFRET method greatly improved the measured E values. Collectively, IIem-spFRET is a powerful and robust tool for quantitatively measuring FRET signal in living cells.

  18. An insight into the immune roles of surfactant proteins, SP-A and SP-D

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    Annapurna eNayak

    2012-06-01

    Full Text Available Pulmonary surfactant proteins, SP-A and SP-D, are hydrophilic collagen-containing calcium-dependent lectins, which appear to have a range of innate immune functions at pulmonary as well as extrapulmonary sites. These proteins bind to target ligands on pathogens, allergens and apoptotic cells via C-terminal carbohydrate recognition domains while the collagen region brings about the effector functions. SP-A and SP-D deals with various pathogens triggering a range of innate immune mechanisms such as agglutination/aggregation, enhancement of phagocytosis and killing reactions, and growth inhibition. SP-A and SP-D have also been involved in the control of pulmonary inflammation including allergy and asthma, and hence, they are likely to central issues in a range of pathological situations. There is also emerging evidence to suggest that SP-A and SP-D are capable of linking innate immunity with adaptive immunity that includes modulation of dendritic cell function and helper T cell polarisation. It is clear now that SP-A and SP-D are potent innate immune molecules in the lungs. This review is a summary of their immunological properties in general and their importance in human health and disease.

  19. Population growth and within-plant distribution of the striped mealybug Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae on cotton

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    Martin D. Oliveira

    2014-03-01

    Full Text Available Population growth and within-plant distribution of the striped mealybug Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae on cotton. The striped mealybug, Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae, is a widely distributed and polyphagous pest species, which naturally occurs on cotton plants in Brazil. This study evaluated the establishment and population growth as well as the within-plant distribution of F. virgata on four cotton cultivars: CNPA 7H (white fibers, BRS Verde, BRS Safira, and BRS Rubi (colored fibers. The experiment was conducted in a complete randomized design with four treatments (cultivars and 18 replications of each. Thus, cotton plants of each cultivar were infested with 100 newly hatched nymphs of F. virgata. The number of adult female mealybugs and the total number of mealybugs per plant were quantified, respectively, at 25 and 50 days after infestation. The developmental and pre-reproductive periods were also determined. Furthermore, we verified the distribution of F. virgata on the plant parts at 25 and 50 days after infestation. Ferrisia virgata showed similar growth of 412-fold in the four cotton cultivars studied. Also, the nymphs were spread on infested leaves; the secondgeneration nymphs were spread and established in all plant parts. Our results characterize F. virgata as having much potential as an important cotton pest in Brazil.

  20. Two new species in the leafhopper genus Pythamus Melichar (Hemiptera: Cicadellidae: Evacanthinae) from China.

    Science.gov (United States)

    Wang, Yang; Zhang, Yalin

    2015-01-01

    Two new leafhopper species, Pythamus hainanensis sp. nov. and Pythamus rufus sp. nov. from China, are described and illustrated. Their diagnostic characteristics are compared with similar species. One new combination, Riseveinus chiabaotawow (Huang, 1992) n. comb., is also proposed. In addition, P. biramosus and P. bispinosus are illustrated based on paratypes. A key to known species of Pythamus is provided, excluding three species for which placement cannot presently be confirmed.

  1. Revision of Poliaspis (Hemiptera, Coccoidea, Diaspididae, with descriptions of 8 new species from Australia

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    Nate Hardy

    2011-10-01

    Full Text Available Eight new Australian species of Poliaspis are described and illustrated: P. alluvia sp. n., P. araucariae sp. n., P. ceraflora sp. n., P. naamba sp. n., P. nalbo sp. n., P. narungga sp. n., P. ozothamnae sp. n., and P. waibenensis sp. n. Two described species are transferred into Poliaspis and are redescribed and illustrated: Lineaspis callitris (Laing originally described by Laing as a species of Poliaspis, is transferred back into Poliaspis as P. callitris Laing comb. rev., and Leonardaspis wilga (Leonardi is transferred to Poliaspis as P. wilga (Leonardi comb. n. Descriptions and illustrations are also provided for six of the fourteen previously-named Poliaspis species, including five from Australia: P. attenuata Brimblecombe, P. elongata Brimblecombe, P. exocarpi Maskell, P. nitens Fuller, and P. syringae Laing. Both P. cycadis Comstock and P. gaultheriae Green become junior synonyms of P. media Maskell. The species not treated here are P. intermedia Fuller (the location of the types is unknown and Fuller’s description is inadequate, P. casuarinicola Lindinger (missing types, P. incisa Takagi and de Faveri (recently, and well described in Takagi and de Faveri 2011, and the six New Zealand species recently revised by Henderson (2011. In addition, Laingaspis lanigera (Laing, the adult female of which has 8 clusters of perivulvar pores – as in Poliaspis species – is redescribed and illustrated. Lectotypes are designated for L. lanigera, P. callitris, P. exocarpi, P. media, and P. wilga. A key is provided to the species of Poliaspis, excluding P. casuarinicola and P. intermedia but including P. incisa and the New Zealand species: P. chathamica Henderson, P. floccosa Henderson, P. lactea (Maskell, P. media Maskell, P. raouliae Henderson and P. salicornicola Henderson.

  2. Sobre o condicionamento alimentar na cochonilha-branca, Planococcus citri (Risso (Hemiptera: pseudococcidae Regarding to host conditioning in citrus mealybug Planococcus citri (Risso (Hemiptera: pseudococcidae

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    Lenira Viana Costa Santa-Cecília

    2013-03-01

    Full Text Available Estudos do comportamento alimentar mediante a técnica de "Electrical Penetration Graphs" (EPG das cochonilhas-farinhentas (Pseudococcidae provenientes de um hospedeiro de criação alternativo têm mostrado que esses insetos não atingem ou demoram cerca de 9 horas para alcançar a fase floemática. Por outro lado, aqueles provenientes do hospedeiro-fonte atingem a fase floemática mais rapidamente e apresentam maior frequência de alimentação nos vasos crivados. Esses resultados indicam a presença do fenômeno de condicionamento alimentar, ainda não demonstrado em cochonilhas. Assim, o presente trabalho teve como objetivo determinar a existência desse fenômeno em Planococcus citri (Risso (Hemiptera: Pseudococcidae. Foram realizados testes de livre escolha, monitoramento eletrônico (EPG e estudos de alguns parâmetros biológicos. Em todos os experimentos, o cafeeiro (Coffea arabica L., os citros (Citrus sinensis L. e abóbora (Cucurbita maxima L. foram utilizados como substratos de criação (fonte da cochonilha, sendo os tratamentos constituídos pela combinação entre os hospedeiros-fonte e os hospedeiros receptores (café e citros. O teste de escolha entre cafeeiro e citros nas primeiras 72 horas mostrou que as cochonilhas criadas em cafeeiro apresentaram preferência pelo cafeeiro; aquelas originadas dos citros mostraram uma tendência, embora não significativa, em selecionar os citros em relação ao cafeeiro e aquelas criadas em abóbora não mostraram preferência por nenhum dos hospedeiros. Os estudos do comportamento alimentar mediante o monitoramento eletrônico (EPG mostraram que a fase floemática, considerada como a fase de aceitação do hospedeiro, foi mais frequente em cafeeiro, seja com cochonilhas oriundas deste substrato, seja de citros. Aqueles insetos mantidos em abóbora e transferidos para o cafeeiro ou citros apresentaram excepcionalmente ou não apresentaram nenhuma fase floemática, respectivamente. A

  3. Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates

    Science.gov (United States)

    Chang, Hong-Yu; Li, Fang; Li, Feng-Sheng; Zheng, Cheng-Zhong; Lei, Yan-Zhe; Wang, Jing

    2016-01-01

    Background We examined selected polymorphisms in 3 pulmonary surfactant-associated proteins (SP) for their influence on serum SP levels and risk of respiratory distress syndrome (RDS) in preterm neonates. Material/Methods Premature infants from a Han population were enrolled, including 100 premature infants with RDS (case group) and 120 premature infants without RDS (control group). SNP genotyping for SP-A (+186A/G and +655C/T), SP-B (−18A/C and 1580C/T), and SP-D (Met11ThrT/C and Ala160ThrG/A) used polymerase chain reaction-restriction fragment length polymorphism. Haplotypes were calculated with Shesis software and serum SP-A/B/D levels were quantified by ELISA. Results Case and control groups exhibited significant differences in genotype and allele frequencies of SP-A (+186A/G, +655C/T) and SP-B (1580C/T). However, no statistically significant differences were observed in the allele and genotype frequencies of SP-B −18A/C, SP-D Met11ThrT/C, and SP-D Ala160ThrG/A. Importantly, serum SP-A and SP-B levels were reduced in RDS patients carrying SP-A (+186A/G, +655C/T) and SP-B (1580C/T) polymorphisms. AA genotype of +186A/G, SP-A level, and CC genotype of 1580C/T were independently correlated with increased RDS risk. Conclusions SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of RDS in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring. PMID:28011976

  4. OCORRÊNCIA DE Gryon gallardoi (BRETHES (HYMENOPTERA: SCELIONIDAE PARASITANDO OVOS DE Leptoglossus zonatus (DALLAS (HEMIPTERA: COREIDAE EM ITUMBIARA, GOIÁS, BRASIL OCCURRENCE OF Gryon gallardi (BRETHES (HYMENOPTERA: SCELIONIDAE PARASITING EGGS OF Leptoglossus zonatus (DALLAS (HEMIPTERA: COREIDAE IN ITUMBIARA, GOIÁS, BRAZIL

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Marchiori

    2007-09-01

    Full Text Available

    Este trabalho relata a primeira ocorrência, em Goiás, de Gryon gallardoi (Bréthes (Hymenoptera: Scelionidae parasitando ovos de Leptoglossus zonatus (Dallas (Hemiptera: Coreidae, em milho cultivar Dekalb 601, no município de Itumbiara, Goiás, Brasil.

    PALAVRAS-CHAVE: Hymenoptera; Hemiptera; parasitóides; controle natural.

    This work reports the first occurrence in Itumbiara, Goiás, Brazil, of Gryon gallardoi (Bréthes (Hymenoptera: Scelionidae parasitizing eggs of Leptoglossus zonatus (Dallas (Hemiptera: Coreidae in maize.

    KEY-WORDS: Hymenoptera; Hemiptera; parasitoids; natural controle.

  5. Metabolites from marine fungus Aspergillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Wahidullah, S.; Rajmanickam, R.; DeSouza, L.

    Chemical examination of a methanolic extract of the marine fungus, Aspergillus sp., isolated from marine grass environment, yielded a steroid, ergosterol peroxide (1), and a mixture of known glyceride esters (2,3) of unsaturated fatty acids...

  6. Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., two tremellaceous yeast species from Brazil.

    Science.gov (United States)

    Machado Pagani, Danielle; Brandão, Luciana R; Santos, Ana Raquel O; Felix, Ciro R; Pais Ramos, Jesus; Broetto, Leonardo; Scorzetti, Gloria; Fell, Jack W; Augusto Rosa, Carlos; Valente, Patricia; Fontes Landell, Melissa

    2016-04-01

    Two yeast species, Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., in the family Rhynchogastremataceae of the Tremellales are proposed. The two species are related to six species of the genus Papiliotrema: Papiliotrema aureus, P. flavescens, P. terrestris, P. baii, P. ruineniae and P. wisconsinensis. The novel species are proposed on the basis of the sequence-based phylogenetic species concept with analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. A total of 16 strains of Papiliotrema leoncinii sp. nov. were obtained from freshwater and bromeliad leaves collected in Brazil. Papiliotrema leoncinii sp. nov. differs by 11, 12, 16, 14, 11 and 13 substitutions in the D1/D2 domain from the related species P. aureus, P. flavescens, P. terrestris, P. baii, P. ruineniae and P. wisconsinensis, respectively. Differences of 11 substitutions and 21 or more substitutions in ITS regions were found when the sequences of Papiliotrema leoncinii sp. nov. were compared with P. wisconsinensis and its closest relatives. The type strain of Papiliotrema leoncinii sp. nov. is UFMG-CM-Y374T (=CBS 13918T). Papiliotrema miconiae sp. nov. is represented by two strains isolated from a flower of Miconia sp. and a water sample in Brazil. Papiliotrema miconiae sp. nov. differs from the related species P. aureus and P. ruineniae by eight substitutions, from P. flavescens and P. terrestris by 11 substitutions, from P. baii by 10 substitutions and from P. wisconsinensis by 6 substitutions in the D1/D2 domain, and by 7 substitutions from P. wisconsinensis and more than 19 substitutions in the ITS region from its closest relatives. The type strain of Papiliotrema miconiae sp. nov. is CBS 8358T (ML 3666T=DBVPG-4492T). The MycoBank numbers for Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov. are MB 813594 and MB 814882, respectively.

  7. Una especie nueva de Discococcus (Hemiptera: Pseudococcidae para la Región Neotropical A new species of Discococcus (Hemiptera: Pseudococcidae from the Neotropical region

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    María Cristina Granara de Willink

    2004-12-01

    Full Text Available Se describen e ilustran la hembra adulta y los estados inmaduros de Discococcus huapi sp. nov. encontrados sobre Stipa speciosa Trinius et Ruprecht en Neuquén; se incluye también una clave de las especies de Discococcus Ferris; este género se cita por primera vez para la región Neotropical.Adult female and inmature stages of Discococcus huapi n. sp. are described and illustrated; it was found on Stipa speciosa Trinius et Ruprecht from Neuquén province; a key of the species of Discococcus Ferris is included; this genus is cited for the first time for the Neotropical Region.

  8. Vorticella sp: Prospective Mosquito Biocontrol Agent

    OpenAIRE

    Patil, Chandrashekhar Devidas; Narkhede, Chandrakant Prakash; Suryawanshi, Rahul Khushal; Patil, Satish Vitthal

    2016-01-01

    Background: Considering the disadvantages of chemical insecticides, we aimed to evaluate Vorticella parasites for control of mosquito larvae of Anopheles stephensi and Aedes aegypti at different larval stages. Methods: Vorticella sp infected mosquito larvae were crushed in the 0.85% saline and homogenized well to get Vorticella in suspension. The effects of Vorticella sp infections on larval development were investigated by inoculating protozoan on different larval instars of An. stephensi an...

  9. Vorticella sp: Prospective Mosquito Biocontrol agent

    OpenAIRE

    Chandrashekhar Devidas Patil; Chandrakant Prakash Narkhede; Rahul Khushal Suryawanshi; Satish Vitthal Patil

    2016-01-01

    Background: Considering the disadvantages of chemical insecticides, we aimed to evaluate Vorticella parasites for control of mosquito larvae of Anopheles stephensi and Aedes aegypti at different larval stages.Methods: Vorticella sp infected mosquito larvae were crushed in the 0.85% saline and homogenized well to get Vorti­cella in suspension. The effects of Vorticella sp infections on larval development were investigated by inoculat­ing protozoan on different larval instars of An. stephensi a...

  10. Review of the genus Relaba (Hemiptera: Cicadellidae: Typhlocybinae: Alebrini) and description of two new species from Argentina.

    Science.gov (United States)

    Catalano, María Inés; Paradell, Susana L; Dietrich, Christopher H

    2016-01-01

    The genus Relaba is reviewed, and two new species, R. forcipula n. sp. and R. globata n. sp. are added. Detailed morphological descriptions and illustrations of the new species, and a key to males of known species are provided.

  11. Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR,two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a ~2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr ~42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56was localized on rat sperm head by the indirect immunofiuorescence method. Rat sp56 immunoreactivitywas detected from the early pachytene spermatocytes and throughout the spermatogenesis. Its cloning willfurther our understanding of the mechanism of the sperm-egg recognition and binding.

  12. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

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    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  13. Four new species of the grass feeding leafhopper genus Nicolaus Lindberg (Hemiptera: Cicadellidae: Deltocephalinae) from the Indian subcontinent.

    Science.gov (United States)

    Viraktamath, C A; Webb, M D

    2014-01-01

    The leafhopper genus Nicolaus Lindberg is recorded from India and Pakistan for the first time. Four new species, N. abuensis sp. nov., N. bidentatus sp. nov., N. cornutus sp. nov. and N. serratus sp. nov. are described and illustrated. N. bihamatus Xing & Li, earlier known from China is recorded from India and Pakistan. A key to the species of Nicolaus from the study area is provided.

  14. Phylogenetic analysis of the true water bugs (Insecta: Hemiptera: Heteroptera: Nepomorpha: evidence from mitochondrial genomes

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    Xie Qiang

    2009-06-01

    Full Text Available Abstract Background The true water bugs are grouped in infraorder Nepomorpha (Insecta: Hemiptera: Heteroptera and are of great economic importance. The phylogenetic relationships within Nepomorpha and the taxonomic hierarchies of Pleoidea and Aphelocheiroidea are uncertain. Most of the previous studies were based on morphological characters without algorithmic assessment. In the latest study, the molecular markers employed in phylogenetic analyses were partial sequences of 16S rDNA and 18S rDNA with a total length about 1 kb. Up to now, no mitochondrial genome of the true water bugs has been sequenced, which is one of the largest data sets that could be compared across animal taxa. In this study we analyzed the unresolved problems in Nepomorpha using evidence from mitochondrial genomes. Results Nine mitochondrial genomes of Nepomorpha and five of other hemipterans were sequenced. These mitochondrial genomes contain the commonly found 37 genes without gene rearrangements. Based on the nucleotide sequences of mt-genomes, Pleoidea is not a member of the Nepomorpha and Aphelocheiroidea should be grouped back into Naucoroidea. Phylogenetic relationships among the superfamilies of Nepomorpha were resolved robustly. Conclusion The mt-genome is an effective data source for resolving intraordinal phylogenetic problems at the superfamily level within Heteroptera. The mitochondrial genomes of the true water bugs are typical insect mt-genomes. Based on the nucleotide sequences of the mt-genomes, we propose the Pleoidea to be a separate heteropteran infraorder. The infraorder Nepomorpha consists of five superfamilies with the relationships (Corixoidea + ((Naucoroidea + Notonectoidea + (Ochteroidea + Nepoidea.

  15. Recilia banda Kramer (Hemiptera: Cicadellidae), a vector of Napier stunt phytoplasma in Kenya

    Science.gov (United States)

    Obura, Evans; Midega, Charles A. O.; Masiga, Daniel; Pickett, John A.; Hassan, Mohamed; Koji, Shinsaku; Khan, Zeyaur R.

    2009-10-01

    Napier grass ( Pennisetum purpureum) is the most important fodder crop in smallholder dairy production systems in East Africa, characterized by small zero-grazing units. It is also an important trap crop used in the management of cereal stemborers in maize in the region. However, production of Napier grass in the region is severely constrained by Napier stunt disease. The etiology of the disease is known to be a phytoplasma, 16SrXI strain. However, the putative insect vector was yet unknown. We sampled and identified five leafhopper and three planthopper species associated with Napier grass and used them as candidates in pathogen transmission experiments. Polymerase chain reaction (PCR), based on the highly conserved 16S gene, primed by P1/P6-R16F2n/R16R2 nested primer sets was used to diagnose phytoplasma on test plants and insects, before and after transmission experiments. Healthy plants were exposed for 60 days to insects that had fed on diseased plants and acquired phytoplasma. The plants were then incubated for another 30 days. Nested PCR analyses showed that 58.3% of plants exposed to Recilia banda Kramer (Hemiptera: Cicadellidae) were positive for phytoplasma and developed characteristic stunt disease symptoms while 60% of R. banda insect samples were similarly phytoplasma positive. We compared the nucleotide sequences of the phytoplasma isolated from R. banda, Napier grass on which these insects were fed, and Napier grass infected by R. banda, and found them to be virtually identical. The results confirm that R. banda transmits Napier stunt phytoplasma in western Kenya, and may be the key vector of Napier stunt disease in this region.

  16. What is the economic threshold of soybean aphids (Hemiptera: Aphididae) in enemy-free space?

    Science.gov (United States)

    McCarville, M T; Kanobe, C; MacIntosh, G C; O'Neal, M

    2011-06-01

    Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a serious pest of soybean, Glycine max (L.) Merr., in the North Central United States. Current management recommendations rely on the application of insecticides based on an economic threshold (ET) of 250 aphids per plant. Natural enemies are important in slowing the increase of aphid populations and can prevent them from reaching levels that can cause economic losses. However, biological control of A. glycines is inconsistent and can be affected negatively by the intensity of agricultural activity. We measured the impact of a natural-enemy-free environment on the capacity of the current ET to limit yield loss. In 2008 and 2009, caged microplots were assigned to one of three treatments: plants kept aphid-free (referred to as the control), plants that experienced a population of 250 aphids per plant (integrated pest management [IPM]), and plants that experienced unlimited aphid population growth (unlimited). The population growth rate of aphids in the unlimited treatment for the 10 d after the application of insecticides to the IPM treatment was calculated using linear regression. The linear equation was solved to determine the mean number of days between the ET and the EIL for an aphid population in absence of predators. The number of days was determined to be 6.97 +/- 1.11 d. The 2-yr average yield for the IPM treatment was 99.93% of the control treatment. Our study suggests the current soybean aphid ET of 250 aphids per plant can effectively protect yield even if the impact of natural enemies is reduced.

  17. Seasonal Effects on the Population, Morphology and Reproductive Behavior of Narnia femorata (Hemiptera: Coreidae).

    Science.gov (United States)

    Cirino, Lauren A; Miller, Christine W

    2017-01-17

    Many insects are influenced by the phenology of their host plants. In North Central Florida, Narnia femorata (Hemiptera: Coreidae) spends its entire life cycle living and feeding on Opuntia mesacantha ssp. lata. This cactus begins producing flower buds in April that lead to unripe green fruit in June that ripen into red fruit through December. Many morphological and behavioral characteristics of N. femorata are known to be affected by cactus phenology in a controlled laboratory setting, including the degree of sexual dimorphism and mating behavior. Our goal with this study was to determine if similar phenotypic changes of N. femorata occurred over time in the wild, and the extent to which these changes were concordant with phenological changes in its host plant. Further, we investigate the length of the insect mouthparts (beak) over time. Ongoing work has suggested that beak length may change across cohorts of developing insects in response to feeding deep within cactus fruit where seed and pulp depth decrease as the fruit ripens. Our results revealed a drop in cactus fruit abundance between the months of July through October 2015 as cactus fruits turned red and ripened. Simultaneously, the average body size of both males and females of N. femorata declined at two sampled sites. Male hind femora (a sexually-selected weapon) decreased disproportionately in size over time so that males later in the year had relatively smaller hind femora for their body size. The sex-specific patterns of morphological change led to increased sexual-size dimorphism and decreased sexual dimorphism for hind femora later in the year. Further, we found that beak length decreased across cohorts of insects as cactus fruit ripened, suggesting phenotypic plasticity in mouthpart length. Behavioral studies revealed that female readiness to mate increased as the season progressed. In sum, we found pronounced changes in the phenotypes of these insects in the field. Although this study is far from

  18. Early-season host switching in Adelphocoris spp. (Hemiptera: Miridae of differing host breadth.

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    Hongsheng Pan

    Full Text Available The mirid bugs Adelphocoris suturalis (Jakovlev, Adelphocoris lineolatus (Goeze and Adelphocoris fasciaticollis (Reuter (Hemiptera: Miridae are common pests of several agricultural crops. These three species have vastly different geographical distributions, phenologies and abundances, all of which are linked to their reliance on local plants. Previous work has shown notable differences in Adelphocoris spp. host use for overwintering. In this study, we assessed the extent to which each of the Adelphocoris spp. relies on some of its major overwinter hosts for spring development. Over the course of four consecutive years (2009-2012, we conducted population surveys on 77 different plant species from 39 families. During the spring, A. fasciaticollis used the broadest range of hosts, as it was found on 35 plant species, followed by A. suturalis (15 species and A. lineolatus (7 species. Abundances of the species greatly differed between host plants, with A. fasciaticollis reaching the highest abundance on Chinese date (Ziziphus jujuba Mill., whereas both A. suturalis and A. lineolatus preferred alfalfa (Medicago sativa L.. The host breadths of the three Adelphocoris spp. differed greatly between subsequent spring and winter seasons. The generalist species exhibited the least host fidelity, with A. suturalis and A. lineolatus using 8 of 22 and 4 of 12 overwinter host species for spring development, respectively. By contrast, the comparative specialist A. fasciaticollis relied on 9 of its 11 overwinter plants as early-season hosts. We highlight important seasonal changes in host breadth and interspecific differences in the extent of host switching behavior between the winter and spring seasons. These findings benefit our understanding of the evolutionary interactions between mirid bugs and their host plants and can be used to guide early-season population management.

  19. Jumping performance of flea hoppers and other mirid bugs (Hemiptera, Miridae).

    Science.gov (United States)

    Burrows, M; Dorosenko, M

    2017-02-13

    The order Hemiptera includes jumping insects with the fastest take-off velocities, all generated by catapult mechanisms. It also contains the large family Miridae or plant bugs. Here we analysed the jumping strategies and mechanisms of six mirid species from high speed videos and from the anatomy of their propulsive legs and conclude that they use a different mechanism in which jumps are powered by the direct contractions of muscles. Three strategies were identified. First, jumping was propelled only by movements of the middle and hind legs which were respectively 140% and 190% longer than the front legs. In three species with masses ranging from 3.4 to 12.2 mg, depression of the coxo-trochanteral and extension of femoro-tibial joints accelerated the body in 8-17 ms to take-off velocities of 0.5 to 0.8 m s(- 1) The middle legs lost ground contact 5-6 ms before take-off so that the hind legs generated the final propulsion. The power requirements could be met by the direct muscle contractions so that catapult mechanisms are not implicated. Second, other species combined the same leg movements with wing beating to generate take-off during a wing downstroke. In the third strategy, up to four wing beat cycles preceded take-off and were not assisted by leg movements. Take-off velocities were reduced and acceleration times lengthened. Other species from the same habitat did not jump. The lower take-off velocities achieved by powering jumping by direct muscle contractions may be offset by eliminating the time taken to load catapult mechanisms.

  20. Seasonal Effects on the Population, Morphology and Reproductive Behavior of Narnia femorata (Hemiptera: Coreidae)

    Science.gov (United States)

    Cirino, Lauren A.; Miller, Christine W.

    2017-01-01

    Many insects are influenced by the phenology of their host plants. In North Central Florida, Narnia femorata (Hemiptera: Coreidae) spends its entire life cycle living and feeding on Opuntia mesacantha ssp. lata. This cactus begins producing flower buds in April that lead to unripe green fruit in June that ripen into red fruit through December. Many morphological and behavioral characteristics of N. femorata are known to be affected by cactus phenology in a controlled laboratory setting, including the degree of sexual dimorphism and mating behavior. Our goal with this study was to determine if similar phenotypic changes of N. femorata occurred over time in the wild, and the extent to which these changes were concordant with phenological changes in its host plant. Further, we investigate the length of the insect mouthparts (beak) over time. Ongoing work has suggested that beak length may change across cohorts of developing insects in response to feeding deep within cactus fruit where seed and pulp depth decrease as the fruit ripens. Our results revealed a drop in cactus fruit abundance between the months of July through October 2015 as cactus fruits turned red and ripened. Simultaneously, the average body size of both males and females of N. femorata declined at two sampled sites. Male hind femora (a sexually-selected weapon) decreased disproportionately in size over time so that males later in the year had relatively smaller hind femora for their body size. The sex-specific patterns of morphological change led to increased sexual-size dimorphism and decreased sexual dimorphism for hind femora later in the year. Further, we found that beak length decreased across cohorts of insects as cactus fruit ripened, suggesting phenotypic plasticity in mouthpart length. Behavioral studies revealed that female readiness to mate increased as the season progressed. In sum, we found pronounced changes in the phenotypes of these insects in the field. Although this study is far from

  1. Resistance of Dusky Cotton Bug, Oxycarenus hyalinipennis Costa (Lygaidae: Hemiptera), to Conventional and Novel Chemistry Insecticides.

    Science.gov (United States)

    Ullah, Saif; Shad, Sarfraz Ali; Abbas, Naeem

    2016-02-01

    The dusky cotton bug, Oxycarenus hyalinipennis Costa (Lygaidae: Hemiptera), is polyphagous in nature and has become one of the severe sucking pests of cotton in Pakistan. O. hyalinipennis has the potential to develop resistance to a number of insecticides, and as a result, O. hyalinipennis outbreaks occur. There is no previous study from Pakistan regarding O. hyalinipennis resistance to insecticides. Therefore, the aim of this study was to assess the resistance of different field populations of O. hyalinipennis to conventional (bifenthrin, deltamethrin, lambda-cyhalothrin, profenofos, triazophos) and novel chemistry (emamectin benzoate, spinosad, chlorfenapyr, imidacloprid, and nitenpyram) insecticides. Five populations of O. hyalinipennis, collected from Multan, Khanewal, Muzaffargarh, Lodhran, and Bahawalpur, were tested for resistance to selected insecticides by the leaf dip method. For three pyrethroids, the resistance ratios were in the range of 14- to 30-fold for bifenthrin, 2.14- to 8.41-fold for deltamethrin, and 9.12- to 16-fold for lambda-cyhalothrin, compared with the laboratory susceptible strain (Lab-PK). For two organophosphates, the range of resistance ratios was 12- to 14-fold for profenofos and 9.04- to 15-fold for triazophos. For five novel chemistry insecticides, the range of resistance ratios was 4.68- to 9.83-fold for emamectin benzoate, 6.38- to 17-fold for spinosad, 16- to 46-fold for chlorfenapyr, 11- to 22-fold for imidacloprid, and 1.32- to 11-fold for nitenpyram. Regular assessment of resistance to insecticides and integrated management plans like judicious use of insecticides and rotation of insecticides along with different modes of action are required to delay resistance development in O. hyalinipennis.

  2. Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome.

    Science.gov (United States)

    Li, Fengqi; Wang, Ran; Qu, Cheng; Fu, Ningning; Luo, Chen; Xu, Yihua

    2016-01-01

    The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNA-seq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.

  3. Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae with Mycobacterium leprae Indicates Potential for Leprosy Transmission.

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    Arthur da Silva Neumann

    Full Text Available Leprosy is a chronic dermato-neurological disease caused by infection with Mycobacterium leprae. In 2013 almost 200,000 new cases of leprosy were detected around the world. Since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. Although leprosy is one of the oldest records of human disease, the mechanisms involved with its transmission and epidemiology are still not completely understood. In the present work, we experimentally investigated the hypothesis that the mosquitoes Aedes aegypti and Culex quinquefasciatus and the hemiptera Rhodnius prolixus act as leprosy vectors. By means of real-time PCR quantification of M. leprae 16SrRNA, we found that M. leprae remained viable inside the digestive tract of Rhodnius prolixus for 20 days after oral infection. In contrast, in the gut of both mosquito species tested, we were not able to detect M. leprae RNA after a similar period of time. Inside the kissing bug Rhodnius prolixus digestive tract, M. leprae was initially restricted to the anterior midgut, but gradually moved towards the hindgut, in a time course reminiscent of the life cycle of Trypanosoma cruzi, a well-known pathogen transmitted by this insect. The maintenance of M. leprae infectivity inside the digestive tract of this kissing bug is further supported by successful mice footpad inoculation with feces collected 20 days after infection. We conclude that Rhodnius prolixus defecate infective M. leprae, justifying the evaluation of the presence of M. leprae among sylvatic and domestic kissing bugs in countries endemic for leprosy.

  4. Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission

    Science.gov (United States)

    Neumann, Arthur da Silva; Dias, Felipe de Almeida; Ferreira, Jéssica da Silva; Fontes, Amanda Nogueira Brum; Rosa, Patricia Sammarco; Macedo, Rafael Enrique; Oliveira, José Henrique; Teixeira, Raquel Lima de Figueiredo; Pessolani, Maria Cristina Vidal; Moraes, Milton Ozório; Suffys, Philip Noel; Oliveira, Pedro L.; Sorgine, Marcos Henrique Ferreira; Lara, Flavio Alves

    2016-01-01

    Leprosy is a chronic dermato-neurological disease caused by infection with Mycobacterium leprae. In 2013 almost 200,000 new cases of leprosy were detected around the world. Since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. Although leprosy is one of the oldest records of human disease, the mechanisms involved with its transmission and epidemiology are still not completely understood. In the present work, we experimentally investigated the hypothesis that the mosquitoes Aedes aegypti and Culex quinquefasciatus and the hemiptera Rhodnius prolixus act as leprosy vectors. By means of real-time PCR quantification of M. leprae 16SrRNA, we found that M. leprae remained viable inside the digestive tract of Rhodnius prolixus for 20 days after oral infection. In contrast, in the gut of both mosquito species tested, we were not able to detect M. leprae RNA after a similar period of time. Inside the kissing bug Rhodnius prolixus digestive tract, M. leprae was initially restricted to the anterior midgut, but gradually moved towards the hindgut, in a time course reminiscent of the life cycle of Trypanosoma cruzi, a well-known pathogen transmitted by this insect. The maintenance of M. leprae infectivity inside the digestive tract of this kissing bug is further supported by successful mice footpad inoculation with feces collected 20 days after infection. We conclude that Rhodnius prolixus defecate infective M. leprae, justifying the evaluation of the presence of M. leprae among sylvatic and domestic kissing bugs in countries endemic for leprosy. PMID:27203082

  5. The biology and thermal requirements of the fennel aphid Hyadaphis foeniculi (Passerini (Hemiptera: Aphididae.

    Directory of Open Access Journals (Sweden)

    José B Malaquias

    Full Text Available The relationship between the insect development rate and temperature was established very early and represents an important ecological variable for modeling the population dynamics of insects. The accurate determination of thermal constant values and the lower and upper developmental thresholds of Hyadaphis foeniculi (Passerini (Hemiptera: Aphididae on fennel (Foeniculum vulgare Miller (Apiales: Apiaceae crops would obviously benefit the effective application of control measures. This paper is a study of the biology and thermal requirements of H. foeniculi. Winged insects were collected from fennel crops at the Embrapa Algodão in Campina Grande, Paraíba. Nymphs (age ≤24 h produced by winged insects were subjected to constant temperatures of 15, 20, 25, 28, 30 or 33°C, a photophase of 12 h and a relative humidity of 70±10%. The results of the study showed that at temperatures between 15 and 30°C, H. foeniculi nymphs were able to develop normally. The four instars were found at all temperatures tested. However, temperatures of 3 and 33°C were lethal to the nymphs. The nymph stage development time varied from 5 (30°C to 19 (15°C days. The influence of temperature on the development time is dependent on the instar. The base temperature (Tb and the thermal constant (K for the nymph stage were estimated at 11.2°C and 107.5 degree-days, respectively. The shortest nymph development stage was observed at 30°C, and the highest nymph viability (85.0% was observed at 28°C. This information can be used for developing phenological models based on the temperature and development rate relationships so that outbreaks of H. foeniculi in the fennel crop can be predicted, therefore improving the application of control programs targeting this fennel pest.

  6. Crop Loss Relationships and Economic Injury Levels for Ferrisia gilli (Hemiptera: Pseudococcidae) Infesting Pistachio in California.

    Science.gov (United States)

    Haviland, David R; Beede, Robert H; Daane, Kent M

    2015-12-01

    Ferrisia gilli Gullan (Hemiptera: Pseudococcidae) is a new pest in California pistachios, Pistacea vera L. We conducted a 3-yr field study to determine the type and amount of damage caused by F. gilli. Using pesticides, we established gradients of F. gilli densities in a commercial pistachio orchard near Tipton, CA, from 2005 to 2007. Each year, mealybug densities on pistachio clusters were recorded from May through September and cumulative mealybug-days were determined. At harvest time, nut yield per tree (5% dried weight) was determined, and subsamples of nuts were evaluated for market quality. Linear regression analysis of cumulative mealybug-days against fruit yield and nut quality measurements showed no relationships in 2005 and 2006, when mealybug densities were moderate. However, in 2007, when mealybug densities were very high, there was a negative correlation with yield (for every 1,000 mealybug-days, there was a decrease in total dry weight per tree of 0.105 kg) and percentage of split unstained nuts (for every 1,000 mealybug-days, there was a decrease in the percentage of split unstained of 0.560%), and a positive correlation between the percentage of closed kernel and closed blank nuts (for every 1,000 mealybug-days, there is an increase in the percentage of closed kernel and closed blank of 0.176 and 0.283%, respectively). The data were used to determine economic injury levels, showing that for each mealybug per cluster in May there was a 4.73% reduction in crop value associated with quality and a 0.866 kg reduction in yield per tree (4.75%).

  7. Identification of Aphis gossypii Glover (Hemiptera: Aphididae Biotypes from Different Host Plants in North China.

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    Li Wang

    Full Text Available The cotton-melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae, is a polyphagous species with a worldwide distribution and a variety of biotypes. North China is a traditional agricultural area with abundant winter and summer hosts of A. gossypii. While the life cycles of A. gossypii on different plants have been well studied, those of the biotypes of North China are still unclear.Host transfer experiments showed that A. gossypii from North China has two host-specialized biotypes: cotton and cucumber. Based on complete mitochondrial sequences, we identified a molecular marker with five single-nucleotide polymorphisms to distinguish the biotypes. Using this marker, a large-scale study of biotypes on primary winter and summer hosts was conducted. All A. gossypii collected from three primary hosts-hibiscus, pomegranate, and Chinese prickly ash-were cotton biotypes, with more cotton-melon aphids found on hibiscus than the other two species. In May, alate cotton and cucumber biotypes coexisted on cotton and cucumber seedlings, but each preferred its natal host. Both biotypes existed on zucchini, although the cucumber biotype was more numerous. Aphids on muskmelon were all cucumber biotypes, whereas most aphids on kidney bean were cotton biotypes. Aphids on seedlings of potato and cowpea belong to other species. In August, aphids on cotton and cucumber were the respective biotypes, with zucchini still hosting both biotypes as before. Thus, the biotypes had different fitnesses on different host plants.Two host-specialized biotypes (cotton and cucumber are present in North China. Hibiscus, pomegranate, and Chinese prickly ash can serve as winter hosts for the cotton biotype but not the cucumber biotype in North China. The fitnesses of the two host-specialized biotypes differ on various summer hosts. When alate aphids migrate to summer hosts, they cannot accurately land on the corresponding plant.

  8. Effects of thiamethoxam seed treatments on soybean aphid (Hemiptera: Aphididae) feeding behavior.

    Science.gov (United States)

    Stamm, M D; Heng-Moss, T M; Baxendale, F P; Reese, J C; Siegfried, B D; Hunt, T E; Gaussoin, R E; Blankenship, E E

    2013-12-01

    Since its discovery in North America in 2000, the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), has rapidly become an important pest of soybean [Glycine max (L.) Merrill], sometimes resulting in significant yield losses. Previous research has documented the toxicity of neonicotinoid seed treatments to soybean aphids, but control under field conditions has been inconsistent. Imidacloprid, a popular neonicotinoid insecticide, has been shown to exhibit antifeedant effects on aphids. Antifeedant activity has not been demonstrated for other neonicotinoids, including thiamethoxam. This research investigated the effects of a thiamethoxam seed treatment on soybean aphid feeding behavior by using electronic penetration graphs (EPG) to visualize stylet penetration behavior. Soybean aphid feeding behavior was assessed for 9 h on thiamethoxam-treated and untreated soybeans (V2 and V4 stages). Because results were inconclusive from initial experiments, a study was conducted to document the effects of thiamethoxam-treated soybeans on soybean aphid survival. The seed treatment was shown to negatively affect aphid survival at 4, 8, and 11 d after aphid introduction. A subsequent EPG study then was designed to document soybean aphid feeding behavior for 15 h, after an initial exposure of 9 h to thiamethoxam-treated soybeans. In this study, the exposed aphids exhibited significant differences in feeding behavior compared with those aphids feeding on untreated soybeans. Soybean aphids on thiamethoxam-treated soybeans spent significantly less time feeding in the sieve element phase, with a greater duration of nonprobing events. These studies suggest soybean aphids are unable to ingest phloem sap, which may be another important element in seed treatment protection.

  9. The biology and thermal requirements of the fennel aphid Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae).

    Science.gov (United States)

    Malaquias, José B; Ramalho, Francisco S; Lira, Aline C S; Oliveira, Flávia Q; Fernandes, Francisco S; Godoy, Wesley A C; Zanuncio, José C

    2014-01-01

    The relationship between the insect development rate and temperature was established very early and represents an important ecological variable for modeling the population dynamics of insects. The accurate determination of thermal constant values and the lower and upper developmental thresholds of Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae) on fennel (Foeniculum vulgare Miller (Apiales: Apiaceae)) crops would obviously benefit the effective application of control measures. This paper is a study of the biology and thermal requirements of H. foeniculi. Winged insects were collected from fennel crops at the Embrapa Algodão in Campina Grande, Paraíba. Nymphs (age ≤24 h) produced by winged insects were subjected to constant temperatures of 15, 20, 25, 28, 30 or 33°C, a photophase of 12 h and a relative humidity of 70±10%. The results of the study showed that at temperatures between 15 and 30°C, H. foeniculi nymphs were able to develop normally. The four instars were found at all temperatures tested. However, temperatures of 3 and 33°C were lethal to the nymphs. The nymph stage development time varied from 5 (30°C) to 19 (15°C) days. The influence of temperature on the development time is dependent on the instar. The base temperature (Tb) and the thermal constant (K) for the nymph stage were estimated at 11.2°C and 107.5 degree-days, respectively. The shortest nymph development stage was observed at 30°C, and the highest nymph viability (85.0%) was observed at 28°C. This information can be used for developing phenological models based on the temperature and development rate relationships so that outbreaks of H. foeniculi in the fennel crop can be predicted, therefore improving the application of control programs targeting this fennel pest.

  10. Methyl salicylate attracts natural enemies and reduces populations of soybean aphids (Hemiptera: Aphididae) in soybean agroecosystems.

    Science.gov (United States)

    Mallinger, Rachel E; Hogg, David B; Gratton, Claudio

    2011-02-01

    Methyl salicylate, an herbivore-induced plant volatile, has been shown to attract natural enemies and affect herbivore behavior. In this study, methyl salicylate was examined for its attractiveness to natural enemies of the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), and for its direct effects on soybean aphid population growth rates. Methyl salicylate lures were deployed in plots within organic soybean [Glycine max (L.) Merr.] fields. Sticky card traps adjacent to and 1.5 m from the lure measured the relative abundance of natural enemies, and soybean aphid populations were monitored within treated and untreated plots. In addition, exclusion cage studies were conducted to determine methyl salicylate's effect on soybean aphid population growth rates in the absence of natural enemies. Significantly greater numbers of syrphid flies (Diptera: Syrphidae) and green lacewings (Neuroptera: Chrysopidae) were caught on traps adjacent to the methyl salicylate lure, but no differences in abundance were found at traps 1.5 m from the lure. Furthermore, abundance of soybean aphids was significantly lower in methyl salicylate-treated plots. In exclusion cage studies, soybean aphid numbers were significantly reduced on treated soybean plants when all plants were open to natural enemies. When plants were caged, however, soybean aphid numbers and population growth rates did not differ between treated and untreated plants suggesting no effect of methyl salicylate on soybean aphid reproduction and implicating the role of natural enemies in depressing aphid populations. Although aphid populations were reduced locally around methyl salicylate lures, larger scale studies are needed to assess the technology at the whole-field scale.

  11. Relationship of soybean aphid (Hemiptera: Aphididae) to soybean plant nutrients, landscape structure, and natural enemies.

    Science.gov (United States)

    Noma, Takuji; Gratton, Claudio; Colunga-Garcia, Manuel; Brewer, Michael J; Mueller, Emily E; Wyckhuys, Kris A G; Heimpel, George E; O'Neal, Matthew E

    2010-02-01

    In the north central United States, populations of the exotic soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), are highly variable across space, complicating effective aphid management. In this study we examined relationships of plant nutrients, landscape structure, and natural enemies with soybean aphid abundance across Iowa, Michigan, Minnesota, and Wisconsin, representing the range of conditions where soybean aphid outbreaks have occurred since its introduction. We sampled soybean aphid and its natural enemies, quantified vegetation land cover and measured soybean nutrients (potassium [K] and nitrogen [N]) in 26 soybean sites in 2005 and 2006. Multiple regression models found that aphid abundance was negatively associated with leaf K content in 2005, whereas it was negatively associated with habitat diversity (Simpson's index) and positively associated with leaf N content in 2006. These variables accounted for 25 and 27% of aphid variability in 2005 and 2006, respectively, suggesting that other sources of variability are also important. In addition, K content of soybean plants decreased with increasing prevalence of corn-soybean cropland in 2005, suggesting that landscapes that have a high intensification of agriculture (as indexed by increasing corn and soybean) are more likely to have higher aphid numbers. Soybean aphid natural enemies, 26 species of predators and parasitoids, was positively related to aphid abundance; however, enemy-to-aphid abundance ratios were inversely related to aphid density, suggesting that soybean aphids are able to escape control by resident natural enemies. Overall, soybean aphid abundance was most associated with soybean leaf chemistry and landscape heterogeneity. Agronomic options that can ameliorate K deficiency and maintaining heterogeneity in the landscape may reduce aphid risk.

  12. Mating disruption of Planococcus ficus (Hemiptera: Pseudococcidae) in vineyards using reservoir pheromone dispensers.

    Science.gov (United States)

    Cocco, Arturo; Lentini, Andrea; Serra, Giuseppe

    2014-10-15

    Mating disruption field experiments to control the vine mealybug, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae), were carried out in 2008 and 2009 in two commercial vineyards in Sardinia (Italy). The effectiveness of mating disruption was evaluated by testing reservoir dispensers loaded with 100 mg (62.5 g/ha) and 150 mg (93.8 g/ha) of the sex pheromone in 2008 and 2009, respectively. The number of males captured in pheromone traps, the P. ficus population density and age structure, the parasitism rate, the percentage of ovipositing females, and the crop damage were compared between disrupted and untreated plots. In both field trials, the number of males captured in mating disruption plots was significantly reduced by 86% and 95%, respectively. Mating disruption at the initial dose of 62.5 g/ha of active ingredient gave inconclusive results, whereas the dose of 93.8 g/ha significantly lowered the mealybug density and modified the age structure, which showed a lower percentage of ovipositing females and a higher proportion of preovipositing females. Mating disruption did not affect negatively the parasitism rate, which was higher in the disrupted than in the control plots (>1.5-fold). Crop damage at harvest was very low in both field trials and did not differ between treatments. Mating disruption was effective in wide plots protected with dispensers loaded with 150 mg of the sex pheromone, showing its potential to be included in the overall integrated control programs in Mediterranean wine-growing regions.

  13. Comparison of sex pheromone traps for monitoring pink hibiscus mealybug (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Vitullo, Justin; Wang, Shifa; Zhang, Aijun; Mannion, Catharine; Bergh, J Christopher

    2007-04-01

    The pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), is a highly polyphagous pest that invaded Florida in 2002 and has recently been reported from several locations in Louisiana. Although identification of its sex pheromone in 2004 improved monitoring capabilities tremendously, the effectiveness and efficiency of different pheromone trap designs for capturing males has not been evaluated. We deployed green Delta, Pherocon IlB, Pherocon V, Jackson, and Storgard Thinline traps in Homestead, FL, and compared the number of male M. hirsutus captured per trap, the number captured per unit of trapping surface area, the amount of extraneous material captured, and the time taken to count trapped mealybugs. Pheromone-baited traps with larger trapping surfaces (green Delta, Pherocon IIB, and Pherocon V) captured more males per trap than those with smaller surfaces (Jackson and Storgard Thinline), and fewest males were captured by Storgard Thinline traps. However, Jackson traps captured as many or more males per square centimeter of trapping surface as those with larger surfaces, and the time required to count males in Jackson traps was significantly less than in green Delta, Pherocon IIB, and Pherocon V traps. Although all trap designs accumulated some debris and nontarget insects, it was rated as light to moderate for all designs. Based on our measures of effectiveness and efficiency, the Jackson trap is most suitable for monitoring M. hirsutus populations. Additionally, unlike the other traps evaluated, which must be replaced entirely or inspected in the field and then redeployed, only the sticky liners of Jackson traps require replacement, enhancing the efficiency of trap servicing.

  14. Effect of temperature on the life history of the mealybug Paracoccus marginatus (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Amarasekare, Kaushalya G; Chong, Juang-Horng; Epsky, Nancy D; Mannion, Catharine M

    2008-12-01

    Effect of temperature on the life history of the mealybug Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae) was investigated in the laboratory. P. marginatus was able to develop and complete its life cycle at 18, 20, 25, and 30 +/- 1 degrees C. At 15, 34, and 35 degrees C, the eggs hatched after 27.5, 5.9, and 5.5 d of incubation, respectively, but further development of the first-instar nymphs was arrested. No eggs hatched at 37 degrees C. The developmental time for egg to adult was the longest at 18 degrees C for both males and females. Approximately 80-90% of the eggs survived between 20 and 30 degrees C. The highest fecundity was at 25 degrees C with each female producing an average of 300 eggs. Adult longevity, and preoviposition and oviposition periods increased with decreasing temperature up to 25 degrees C. The proportion of females was approximately 42% at 25 degrees C and was between 70 and 80% at 18, 20, and 30 degrees C. Adult males and females required 303.0 and 294.1 degree-days (DD), respectively, to complete their development. The estimated minimum temperature thresholds for the adult males and females were 14.5 and 13.9 degrees C, respectively. For adult males, the estimated optimum and maximum temperature thresholds were 28.7 and 31.9 degrees C; and for adult females, they were 28.4 and 32.1 degrees C, respectively. The ability of P. marginatus to develop, survive, and reproduce successfully between 18 and 30 degrees C suggests that it has the capability to develop and establish in areas within this temperature range.

  15. Pheromone-based mating disruption of Planococcus ficus (Hemiptera: Pseudococcidae) in California vineyards.

    Science.gov (United States)

    Walton, Vaughn M; Daane, Kent M; Bentley, Walter J; Millar, Jocelyn G; Larsen, Thomas E; Malakar-Kuenen, Raksha

    2006-08-01

    Experiments were conducted to test a mating disruption program for the mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) in California vineyards. The sprayable, microencapsulated formulation of the racemic sex pheromone lavandulyl senecioate was applied with an air-blast sprayer, using three and four applications in 2003 and 2004, respectively. Mating disruption was combined with an application of buprofezin (2004) in June. Compared with a no-pheromone control, there were significantly lower season-long trap catches of adult males, season-long mealybug densities (2003 only), and crop damage in mating disruption plots. The amount of mealybug reduction and mechanisms that resulted in lower crop damage in mating disruption plots is discussed. In samples taken during the growing season (April to September), mealybug density was only 12.0 +/- 15.6 and 31.1 +/- 11.6% lower in the mating disruption plots than in control plots in 2003 and 2004, respectively. In the mating disruption treatment, mealybug egg production was significantly lower (2003 only), as were the proportion of ovisacs and crawlers produced. There was no treatment impact on percentage of parasitism. Mealybug density influenced treatment impact. In 2004, vines were categorized as having low, medium, or high mealybug densities during a preapplication survey. After treatment application, mealybug density was reduced by 86.3 +/- 6.3% on vines in the low mealybug density category, but it was unchanged on vines in the high density category. Another factor that reduced treatment impact was the relatively short effective lifetime of the sprayable formulation.

  16. DNA barcoding of common soft scales (Hemiptera: Coccoidea: Coccidae) in China.

    Science.gov (United States)

    Wang, X-B; Deng, J; Zhang, J-T; Zhou, Q-S; Zhang, Y-Z; Wu, S-A

    2015-10-01

    The soft scales (Hemiptera: Coccoidea: Coccidae) are a group of sap-sucking plant parasites, many of which are notorious agricultural pests. The quarantine and economic importance of soft scales necessitates rapid and reliable identification of these taxa. Nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene (barcoding region) and 28S rDNA were generated from 340 individuals of 36 common soft scales in China. Distance-based [(best match, Automated Barcode Gap Discovery (ABGD)], tree-based (neighbor-joining, Bayesian inference), Klee diagrams, and general mixed Yule coalescent (GMYC) models were used to evaluate barcoding success rates in the data set. Best match showed that COI and 28S sequences could provide 100 and 95.52% correct identification, respectively. The average interspecific divergences were 19.81% for COI data and 20.38% for 28S data, and mean intraspecific divergences were 0.56 and 0.07%, respectively. For COI data, multiple methods (ABGD, Klee, and tree-based methods) resulted in general congruence with morphological identifications. However, GMYC analysis tended to provide more molecular operational taxonomic units (MOTUs). Twelve MOTUs derived from five morphospecies (Rhodococcus sariuoni, Pulvinaria vitis, Pulvinaria aurantii, Parasaissetia nigra, and Ceroplastes rubens) were observed using the GMYC approach. In addition, tree-based methods showed that 28S sequences could be used for species-level identification (except for Ceroplastes ceriferus - Ceroplastes pseudoceriferus), even with low genetic variation (<1%). This report demonstrates the robustness of DNA barcoding for species discrimination of soft scales with two molecular markers (COI and 28S) and provides a reliable barcode library and rapid diagnostic tool for common soft scales in China.

  17. Utilizing immunomarking techniques to track Halyomorpha halys (Hemiptera: Pentatomidae movement and distribution within a peach orchard

    Directory of Open Access Journals (Sweden)

    Brett R. Blaauw

    2016-05-01

    Full Text Available In this study we focus on the invasive brown marmorated stink bug, Halyomorpha halys (Stål (Hemiptera: Pentatomidae, which has a strong dispersal capacity and has had a significant impact on several cropping systems, including peach (Prunus persica (L.. Management of H. halys has relied on intensive insecticide use, and thus a better understanding of its dispersal behavior may assist in developing improved management strategies. In order to investigate H. halys movement and distribution patterns within a peach orchard we applied ecologically safe, food protein markers to the trees along the orchard border (chicken egg albumin in the form of liquid egg whites and to the trees within the orchard interior (bovine casein in the form of cow’s milk. We used enzyme-linked immunosorbent assays (ELISA to assess whether collected H. halys were “marked” with either of the two protein markers, revealing where in the orchard the bugs had visited. From the density data we determined that H. halys is a perimeter-driven pest in peaches, with a significantly higher density of bugs collected along the orchard border. Interestingly, this trend is primarily driven by the distribution of male bugs. The protein marking data revealed that a small proportion of male H. halys move equally between the orchard border and interior, while a small proportion of females move predominately to the border after visiting the interior. The verification of a strong edge-effect, although potentially sex-specific, implies that H. halys displays a dispersal behavior that may also be exploited for management, which may help growers more efficiently and more effectively manage H. halys.

  18. Lack of fitness costs associated with acetamiprid resistance in Bemisia tabaci (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Basit, Muhammad; Sayyed, Ali H; Saeed, Shafqat; Saleem, Mushtaq A

    2012-08-01

    Sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a devastating pest that can cause severe damage to a range of crops by direct feeding and by plant virus transmission. Because of indiscriminate use of insecticides, this whitefly has developed resistance to several insecticides, including neonicotinoids. Our objectives were to determine fitness components affected by acetamiprid resistance in B. tabaci. Assay results showed that selection with acetamiprid had removed heterozygotes from the field population because the survival rate of the resistant population was significantly greater than that of the field population at a very high dose. Comparison of various life traits between the acetamiprid-selected (Aceta-SEL) population and three other populations showed that the numbers of eggs laid by acetamiprid Aceta-SEL population were significantly lower compared with that of other populations but that the proportions of eggs hatched were significantly higher. However, the time taken by nymphal stages of the Aceta-SEL population to develop was significantly higher than that of the susceptible populations. The intrinsic rate of increase, net reproductive rate, mean generation time, and doubling time of Aceta-SEL was significantly higher than Lab-PK and UNSEL populations, but the growth index was similar for all populations. The growth index and high intrinsic value of Aceta-SEL population suggest that the resistance allele may not have detrimental impact. The lack of fitness costs in B. tabaci could promote the rapid development of resistance to acetamiprid and other neonicotinoids. This resistance could threaten the sustainability of whitefly management program on genetically engineered cotton (Gossypium hirsutum L.) where neonicotinoids are being sprayed to manage sucking pests in the field.

  19. Seasonal Effects on the Population, Morphology and Reproductive Behavior of Narnia femorata (Hemiptera: Coreidae

    Directory of Open Access Journals (Sweden)

    Lauren A. Cirino

    2017-01-01

    Full Text Available Many insects are influenced by the phenology of their host plants. In North Central Florida, Narnia femorata (Hemiptera: Coreidae spends its entire life cycle living and feeding on Opuntia mesacantha ssp. lata. This cactus begins producing flower buds in April that lead to unripe green fruit in June that ripen into red fruit through December. Many morphological and behavioral characteristics of N. femorata are known to be affected by cactus phenology in a controlled laboratory setting, including the degree of sexual dimorphism and mating behavior. Our goal with this study was to determine if similar phenotypic changes of N. femorata occurred over time in the wild, and the extent to which these changes were concordant with phenological changes in its host plant. Further, we investigate the length of the insect mouthparts (beak over time. Ongoing work has suggested that beak length may change across cohorts of developing insects in response to feeding deep within cactus fruit where seed and pulp depth decrease as the fruit ripens. Our results revealed a drop in cactus fruit abundance between the months of July through October 2015 as cactus fruits turned red and ripened. Simultaneously, the average body size of both males and females of N. femorata declined at two sampled sites. Male hind femora (a sexually-selected weapon decreased disproportionately in size over time so that males later in the year had relatively smaller hind femora for their body size. The sex-specific patterns of morphological change led to increased sexual-size dimorphism and decreased sexual dimorphism for hind femora later in the year. Further, we found that beak length decreased across cohorts of insects as cactus fruit ripened, suggesting phenotypic plasticity in mouthpart length. Behavioral studies revealed that female readiness to mate increased as the season progressed. In sum, we found pronounced changes in the phenotypes of these insects in the field. Although this

  20. Characterization of Paecilomycescinnamomeus from the camellia whitefly, Aleurocanthus camelliae (Hemiptera: Aleyrodidae), infesting tea in Japan.

    Science.gov (United States)

    Saito, Tsutomu; Takatsuka, Jun; Shimazu, Mitsuaki

    2012-05-01

    The whitefly, Aleurocanthus camelliae Kanmiya and Kasai (Hemiptera: Aleyrodidae), is an invasive species in Japan that was first discovered in 2004 on tea in Kyoto. Soon after its arrival epizootics of an entomopathogenic fungus were observed in populations of the whitefly in many tea-growing regions. Here we identify this fungus as Paecilomyces cinnamomeus (Petch) Samson and W. Gams (Hypocreales: Clavicipitaceae) based on morphological characteristics and molecular analyses. This is the first record of P. cinnamomeus in Japan and also the first time it has been recorded from the genus Aleurocanthus. A isolate of P. cinnamomeus caused greater than 50% and 90% infection in whitefly nymphs at 1×10(6) and 1×10(7)conidia/ml respectively, while the commercial mycoinsecticides Preferd® (Isaria fumosorosea) and Mycotal® (Lecanicillium muscarium) caused <10% infection at their recommended field rates (5×10(6) and 9×10(6)conidia/ml, respectively), suggesting that P. cinnamomeus may be more useful as a control agent than the currently available mycoinsecticides. Optimum and upper limit temperatures for in vitro growth of P. cinnamomeus isolates were 22.5-25°C and 32.5°C, respectively. At field rates, the fungicide thiophanate-methyl caused some inhibition of in vitro growth of P. cinnamomeus isolates, and the bactericide copper oxychloride and the insecticides tolfenpyrad and methidathion were strongly inhibitory. The findings obtained in this study will be useful in the development of microbial control programs using P. cinnamomeus against A. camelliae.

  1. Three new species of the leafhopper genus Drabescus Stål (Hemiptera: Cicadellidae: Deltocephalinae) from China.

    Science.gov (United States)

    Wang, Jia-Jia; Qu, Ling; Xing, Ji-Chun; Dai, Ren-Huai

    2016-01-01

    Three new species of leafhoppers: Drabescus cuspidatus sp. nov., Drabescus convolutus sp. nov. and Drabescus multidentatus sp. nov. are described and illustrated from China. A key to 24 of the 30 known Chinese species of the genus is given. The type specimens of the new species are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (GUGC).

  2. Three new species in the subfamily Eriopeltinae Sulc from Italy (Hemiptera, Coccoidea, Coccidae) with comments on the genus Lecanopsis.

    Science.gov (United States)

    Pellizzari, Giuseppina

    2013-01-01

    Three new coccid species, namely Hadzibejliaspis ferenci Pellizzari n. sp., Lecanopsis sicula Pellizzari n. sp. and L. salvatorei Pellizzari n. sp. are described and illustrated. Identification keys for the genera in the subfamily Eriopeltinae Sulc and to species in the genera Hadzibejliaspis Koteja and Lecanopsis Targioni Tozzetti are provided.

  3. Descrição de seis espécies de Chinavia (Hemiptera, Pentatomidae, Pentatominae da América do Sul Description of six new species of Chinavia (Hemiptera, Pentatomidae, Pentatominae from South America

    Directory of Open Access Journals (Sweden)

    Cristiano F. Schwertner

    2006-06-01

    Full Text Available Seis novas espécies de Chinavia Orian, 1965 são descritas: Chinavia vanduzeei sp. nov., do Peru (Madre Dios e Brasil (Pará; Chinavia schuhi sp. nov., do Peru (Loreto, Colombia (Bolívar e Brazil (Amazonas; Chinavia sebastiaoi sp. nov., do Brazil (Mato Grosso do Sul, Bolivia (La Paz e Santa Cruz e Paraguai (Carumbé; Chinavia cearensis sp. nov., Chinavia tuiucauna e Chinavia rufitibia sp. nov., do Brasil (Ceará, Bahia e Paraná, respectivamente. São fornecidas características diagnósticas de cada espécie.Six new species of Chinavia Orian, 1965 are described: Chinavia vanduzeei sp. nov., from Peru (Madre Dios and Brazil (Pará; Chinavia schuhi sp. nov., from Peru (Loreto, Colombia (Bolívar and Brazil (Amazonas; Chinavia sebastiaoi sp. nov., from Brazil (Mato Grosso do Sul, Bolivia (La Paz and Santa Cruz and Paraguai (Carumbé; Chinavia cearensis sp. nov., Chinavia tuiucauna sp. nov., and Chinavia rufitibia sp. nov., from Brazil (Ceará, Bahia and Paraná, respectively. Diagnoses to each species are given.

  4. Chaenorhinum semispeluncarum sp. nov. and C. yildirimlii sp. nov. (Scrophulariaceae) from east Anatolia, Turkey

    DEFF Research Database (Denmark)

    Yildirim, Hasan; Tan, Kit; Senol, Serdar;

    2010-01-01

    Chaenorhinum semispeluncarum H. Yildirim, Kit Tan, S. Senol & A. Pirhan sp. nov. and C. yildirimlii Kit Tan, H. Yildirim, S. Senol & A. Pirhan sp. nov. (Scrophulariaceae, C. sect. Microrrhinum) from east Anatolia are described and illustrated. They are both narrow endemics related to the rare C. ...

  5. Isolation of Bartonella rattimassiliensis sp. nov. and Bartonella phoceensis sp. nov. from European Rattus norvegicus

    OpenAIRE

    Gundi, Vijay A. K. B.; Davoust, Bernard; Khamis, Atieh; Boni, Mickaël; Raoult, Didier; La Scola, Bernard

    2004-01-01

    Thirty-three isolates of Bartonella spp., including 11 isolates not belonging to previously known species, were isolated from 66 Rattus norvegicus subjects trapped in the city of Marseille, France. Based on seven different gene sequences, the 11 isolates were assigned to Bartonella rattimassiliensis sp. nov. and Bartonella phoceensis sp. nov.

  6. Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

    Directory of Open Access Journals (Sweden)

    Khamesipour Faham

    2014-12-01

    Full Text Available The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray. The samples were examined using conventional polymerase chain reaction (PCR. Fourteen (14.89% dogs were positive for Brucella sp. and 18 (19.15%. dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05. However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P < 0.0001; χ2 = 30.3767. The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.

  7. Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov. (Acari: Stigmaeidae) from Kurdistan, Iran.

    Science.gov (United States)

    Khanjani, Mohammad; Nasrollahi, Siamak; Zamani, Ali Sina; Fayaz, Bahman Asali

    2014-07-28

    Two new species belonging to the family Stigmaeidae, Cheylostigmaeus tarae sp. nov. and Stigmaeus delaramae sp. nov., are described from specimens collected from soil and litter under pear trees, Pyrus communis L. (Rosaceae) in Iran. A key to all Iranian species of the genera Cheylostigmaeus (male) and Stigmaeus (female) are provided. 

  8. Efektifitas Insektisida Nabati Lengkuas (Languas galangal L.) dan Serai (Andropogon nardus L.) dalam Mengendalikan Kepik Penghisap Polong Kedelai Riptortus linearis Fabr. (Hemiptera : Alydidae) di Rumah Kasa

    OpenAIRE

    Nainggolan, Shierly Parupa

    2016-01-01

    Shierly Parupa Nainggolan. 2015. “Effectivity of Botanical Insecticides Galanga (Alpinia galangal L.) and Lemon Grass (Andropogon nardus L.) against Soybean Pod Sucking Bugs (Riptortus linearis Fabr.) (Hemiptera : Alydidae) in Screen House“ supervised by Syahrial Oemry and Lisnawita. The objective of the research was to study the effectivity of botanical insecticides galangal and lemongrass to infect the adult soybean pod sucking bugs (Riptortus linearis Fabr.) in screen ...

  9. Primer registro de Acerophagus griseus (Hymenoptera: Encyrtidae como parasitoide de Pseudococcus viburni (Hemiptera: Pseudococcidae en el Alto Valle de Río Negro, Argentina

    Directory of Open Access Journals (Sweden)

    Daniel A. AQUINO

    2013-01-01

    Full Text Available Se cita por primera vez la presencia de Acerophagus griseus (De Santis (Hymenoptera: Encyrtidae como parasitoide de ninfas y adultos de Pseudococcus viburni (Signoret (Hemiptera: Pseudococcidae y se registra la zona de Alto Valle de Río Negro como nuevo registro de distribución en Argentina. Se brinda una diagnosis y se ilustran los caracteres morfológicos de A. griseus.

  10. Complete Genome Sequences of the Obligate Symbionts “Candidatus Sulcia muelleri” and “Ca. Nasuia deltocephalinicola” from the Pestiferous Leafhopper Macrosteles quadripunctulatus (Hemiptera: Cicadellidae)

    Science.gov (United States)

    Abbà, Simona; Kube, Michael; Marzachì, Cristina

    2016-01-01

    Two bacterial symbionts of the European pest leafhopper, Macrosteles quadripunctulatus (Hemiptera: Cicadellidae), were fully sequenced. “Candidatus Sulcia muelleri” and “Ca. Nasuia deltocephalinicola” represent two of the smallest known bacterial genomes at 190 kb and 112 kb, respectively. Genome sequences are nearly identical to strains reported from the closely related host species, M. quadrilineatus. PMID:26798106

  11. Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae? A agressividade da presa altera o comportamento de ataque do predador Supputius cincticeps (Stål (Hemiptera, Pentatomidae?

    Directory of Open Access Journals (Sweden)

    Rafael Braga da Silva

    2012-06-01

    Full Text Available Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae? The stink bug Supputius cincticeps (Stål (Hemiptera, Pentatomidae is a predator found in several Brazilian regions, which possesses desirable attributes as a natural control agent and in biological control programs. The aim of this study was to test if the attack behavior and predation success of S. cincticeps were affected by prey species. Larvae of Tenebrio molitor (L. (Coleoptera, Tenebrionidae, Spodoptera frugiperda (J. E. Smith (Lepidoptera, Noctuidae, and Thyrinteina arnobia (Stoll (Lepidoptera, Geometridae were offered to S. cincticeps in laboratory bioassays where predatory attack and prey defensive behaviors were observed for 2-hour periods. The attack behavior of S. cincticeps changed with the prey species offered. More than 25% of T. molitor and S. frugiperda larvae were immediately attacked, but T. arnobia was not immediately attacked by S. cincticeps. Successful attack (i.e., successful insertion of the predator stylets into the prey depends on the region of the body attacked, with a greater proportion of successful attacks in the anterior than in the median or posterior regions. Larvae of T. arnobia and S. frugiperda displayed a sequence of abrupt head and body movements in response to S. cincticeps attack. Attempts of predation were more successful on T. molitor and S. frugiperda than on T. arnobia. Information about the differential attack behavior of S. cincticeps on different prey species is important for designing successful biological control programs using this hemipteran predator.A agressividade da presa altera o comportamento de ataque do predador Supputius cincticeps (Stål (Hemiptera, Pentatomidae? O percevejo Supputius cincticeps (Stål (Hemiptera, Pentatomidae é um predador encontrado em várias regiões brasileiras, que possui atributos desejáveis como agente de controle natural ou em

  12. Hallazgo de Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae en el estado brasileño de "Rio Grande do Sul" Finding of Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae in the Brazilian State of "Rio Grande do Sul"

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    Roberto Salvatella

    1991-02-01

    Full Text Available Triatoma platensis Neiva 1913 (Hemiptera, Triatominae, especie ornitófila, con área de dispersión conocida en Argentina, Bolivia, Paraguay y Uruguay es notificada en un primer registro para Brasil. Hallada en el municipio de Uruguaiana (Río Grande do Sul en nidos de Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae donde cohabitaba con cricétidos de la especie Orizomys flavescens. Ningún ejemplar de los siete colectados se presentó infectado por Trypanosoma cruzi.Triatoma platensis, an ornitophilic species, found in Argentina, Bolivia, Paraguay and Uruguay is reported for the first time in Brazil. It was found in the county of Uruguaiana (Rio Grande do Sul in nests of Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae where it cohabited with cricetids belonging to the species Orizomys flavescens. None of the seven individuals collected were infected by Trypanosoma cruzi.

  13. Description of two new species of Portanus Ball, 1932 (Hemiptera: Cicadellidae: Portanini) from Northeastern Brazil.

    Science.gov (United States)

    De Souza, Stephanie Pereira; Takiya, Daniela Maeda

    2014-01-01

    Two new species of Portanus Ball, 1932 are described and illustrated from Brazil. P. hastatus sp. nov. from Ceará State, can be recognized by its acute pygofer apex and aedeagus with pair of apical processes directed dorsally. Portanus phoenicius sp. nov. from Piauí State, can be recognized by its rounded pygofer lobe with dorsal tooth turned inwardly, aedeagus apex with pair of elongate processes and pair of rounded flanges directed ventrally. Portanus boliviensis is newly recorded from Brazil. These findings double the number of species of Portanini recorded from Northeastern Brazil. 

  14. Review of the genus Anasta Emeljanov (Hemiptera: Dictyopharidae: Hastini) from the Australasian region.

    Science.gov (United States)

    Song, Zhi-Shun; Liang, Ai-Ping

    2013-11-29

    The dictyopharid planthopper genus Anasta Emeljanov from the Australasian region is revised to include six species including a new one: A. australiaca (Lallemand, 1935) comb. nov. (North Australia), A. lobosa sp. nov. (Papua New Guinea), A. minuta (Lallemand, 1935) comb. nov. (Timor, Indonesia), A. prognatha (Distant, 1906) (North Australia, Papua New Guinea), A. timorina (Lallemand, 1935) (Timor, Indonesia), and A. vitiensis Emeljanov et Wilson, 2009 (Fiji). Descriptions or redescriptions of A. australiaca, A. lobosa sp. nov., and A. prognatha are provided together with dorsal habitus and structural illustrations of male genitalia. A key to the species of the genus and distribution map are provided. The biogeography of the genus is discussed.

  15. Evaluation of nutrient components of Bangia sp.

    Institute of Scientific and Technical Information of China (English)

    李水军; 马家海; 纪焕红; 谢恩义

    2003-01-01

    General nutrient components including moisture, ash, crude protein, lipid and carbohy-drate, inorganic elements, photosynthetic pigments, amino acids and fatty acids of Bangia sp. Were an-alyzed and compared with Porphyra yezoensis. The results indicated that the content of protein, totalamino acids and free amino acids accounted for 47.2 %, 42.6 % and 3.3 % in dried Bangia sp. , respec-tively, all essential amino acids were present in relatively high levels and desirable composition. Lipidwas 1.8 %, in which the eicosapentaenoic acid (EPA) reached 50.5 %. In per 100 g dried material, thecontent of chlorophyll, carotenoids, phycoerythrin and phycocyanin accounted for 766, 208, 4 600 and2 963 mg, respectively. Inorganic elements necessary for human body were found to be abundant inBangia sp. The results showed that the nutritive value of Bangia sp. Was the highest among algae re-ported so far, and the industry of Bangia sp. Was provided with significant economic importance and de-veloping potential.

  16. Interaction of uranium with Pleurotus sp.

    Energy Technology Data Exchange (ETDEWEB)

    Ohnuki, Toshihiko; Sakamoto, Fuminori; Kozaki, Naofumi; Ozaki, Takuro; Samadfam, Mohammad [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    2002-11-01

    Uptake of uranium by higher fungi, such as mushroom is little elucidated. We have studied the interaction of uranium with Pleurotus sp. (a mushroom) in pure culture over a wide range of U concentration (50-3000 mg/L). The Pleurotus sp. was cultured in two different media. One was rice bran medium, and the other was agar (yeast extract, peptone and dextrose) medium. The uptake of uranium in Pleurotus sp. was examined by alpha ray autoradiography (A,A), X-ray fluorescence spectroscopy (XRF) and scanning microcopy (SEM) equipped with EDS. In the agar medium, the higher uranium concentration gave lower growth of mycelia, and no fruiting body was observed. In the rice bran medium, the fruiting body was grown at U concentrations up to 1000 mg/L. The AA and XRF analysis showed that uranium taken up in the fruiting body was below the detection limit. The SEM-EDS analysis indicated that U was distributed in the limited region and was not transported to the mycelia far from U containing medium. It is concluded that uranium affects the growth of Pleurotus sp., and little uranium is taken up by Pleurotus sp. during the growth of both mycelia and fruiting body. (author)

  17. First record of occurrence of mosca-negra-dos-citrus, Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Sternorrhyncha: Aleyrodidae in Roraima, Brazil = Primeiro registro da ocorrência de mosca-negra-dos-citros, Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Aleyrodidae em Roraima

    Directory of Open Access Journals (Sweden)

    Francisco Clemilto da Silva Maciel

    2011-12-01

    Full Text Available The Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae is an important pest of citrus. Originated in Asia, this pest was detected in Brazil for the first time in the State of Para, Brazil in 2001. In the culture of citrus fruits the black fly carries direct and indirect damage, impeding the development and production of plants. In addition to restricting trade in local areas from its occurrence to the presence of the plague, since the A. woglumi quarantine pest is considered present (A2 of high alert in accordance with Instruction No 23, April 29, 2008, established by the Ministério da Agricultura, Pecuária e Abastecimento of the Brazil. The objective of this paper was to record the first occurrence of Aleurocanthus woglumi in the State of Roraima, Brazil.= A mosca-negra-dos-citros, Aleurocanthus woglumi Ashb, (Hemiptera: Aleyrodidae é uma importante praga dos citros. De origem asiática, esta praga foi detectada no Brasil pela primeira vez no estado do Pará no ano de 2001. Na cultura dos citros a mosca-negra acarreta danos diretos e indiretos, prejudicando o desenvolvimento e produção das plantas. Além de restringir o comércio de locais de sua ocorrência para áreas livres da presença da praga, visto que o A. woglumi é considerado praga quarentenária presente (A2 de alerta máximo de acordo com a instrução normativa No 23, de 29 de abril de 2008; estabelecida pelo Ministério da Agricultura, Pecuária e Abastecimento. Objetivou-se com o presente trabalho registrar a primeira ocorrência de mosca-negra-dos-citros no estado de Roraima.

  18. Screening of Metarhizium anisopliae (Metsch. Sorok. strains against Mahanarva fimbriolata (Stal, 1854 (Hemiptera: Cercopidae/ Seleção de isolados de Metarhizium anisopliae (Metsch. Sorok. a Mahanarva fimbriolata (Stal, 1854 (Hemiptera: Cercopidae

    Directory of Open Access Journals (Sweden)

    Solange Aparecida Vieira

    2006-06-01

    Full Text Available The mechanized harvest of sugarcane results in accumulation of mulch on the soil and promotes favorable temperature and moist to development of sugarcane root spittlebug, Mahanarva fimbriolata (Stal, 1854 (Hemiptera: Cercopidae. High populations of this pest causes serious damages to plants and high costs with insecticides. This research work tested strains of Metarhizium anisopliae (Metsch. Sorok. pathogenic to sugarcane root spittlebug using a new methodology. The selection was carried out in laboratory conditions. Nymphs were sprayed with 2 mL of a suspension containing 5x107 conidia/mL and maintained in roots of sugarcane. The corrected mortality was evaluated five days after pulverization and ranged from 10.5 to 60% and the more pathogenic strains caused mortality from 46.5 to 59.7%.A colheita mecanizada de cana-de-açúcar, sem a queima da palhada, proporciona acúmulo de matériaorgânica no solo tornando o ambiente favorável ao desenvolvimento da cigarrinha-da-raiz, Mahanarva fimbriolata (Stal, 1854 (Hemiptera: Cercopidae. Em elevadas infestações essa praga tem causado prejuízos devido aos grandes danos nas plantas e aos gastos com agrotóxicos. O objetivo da pesquisa foi selecionar isolados de Metarhizium anisopliae (Metsch. Sorok. patogênicos para cigarrinha-da-raiz, usando uma nova metodologia. A seleção foi feita em condições de laboratório, utilizando-se ninfas coletadas a campo que foram pulverizadas com o fungo e mantidas em raiz de mudas de cana-de-açúcar. A mortalidade corrigida ao quinto dia após a inoculação variou de 10,5 a 60%, sendo que os isolados mais patogênicos causaram mortalidades de 46,5 a 59,7%.

  19. Metallaphotoredox-catalysed sp3-sp3 cross-coupling of carboxylic acids with alkyl halides

    Science.gov (United States)

    Johnston, Craig P.; Smith, Russell T.; Allmendinger, Simon; MacMillan, David W. C.

    2016-08-01

    In the past 50 years, cross-coupling reactions mediated by transition metals have changed the way in which complex organic molecules are synthesized. The predictable and chemoselective nature of these transformations has led to their widespread adoption across many areas of chemical research. However, the construction of a bond between two sp3-hybridized carbon atoms, a fundamental unit of organic chemistry, remains an important yet elusive objective for engineering cross-coupling reactions. In comparison to related procedures with sp2-hybridized species, the development of methods for sp3-sp3 bond formation via transition metal catalysis has been hampered historically by deleterious side-reactions, such as β-hydride elimination with palladium catalysis or the reluctance of alkyl halides to undergo oxidative addition. To address this issue, nickel-catalysed cross-coupling processes can be used to form sp3-sp3 bonds that utilize organometallic nucleophiles and alkyl electrophiles. In particular, the coupling of alkyl halides with pre-generated organozinc, Grignard and organoborane species has been used to furnish diverse molecular structures. However, the manipulations required to produce these activated structures is inefficient, leading to poor step- and atom-economies. Moreover, the operational difficulties associated with making and using these reactive coupling partners, and preserving them through a synthetic sequence, has hindered their widespread adoption. A generically useful sp3-sp3 coupling technology that uses bench-stable, native organic functional groups, without the need for pre-functionalization or substrate derivatization, would therefore be valuable. Here we demonstrate that the synergistic merger of photoredox and nickel catalysis enables the direct formation of sp3-sp3 bonds using only simple carboxylic acids and alkyl halides as the nucleophilic and electrophilic coupling partners, respectively. This metallaphotoredox protocol is suitable for

  20. A new species of the genus Eurhadina Haupt (Hemiptera: Auchenorrhyncha: Cicadellidae: Typhlocybinae) from Korea, with a key to Korean species.

    Science.gov (United States)

    Oh, Sumin; Lim, Jongok; Jung, Sunghoon

    2016-01-01

    The leafhopper genus Eurhadina Haupt, 1929 belongs to the tribe Typhlocybini of subfamily Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae). Currently, genus Eurhadina includes 3 subgenera, Eurhadina Haupt 1929, Singhardina Mahmood 1967, Zhihadina Yang & Li 1991. A total of 20 valid species of subgenus Eurhadina have been described in the Nearctic and Palaearctic region and the subgenus Singhardina includes 57 species in the Oriental and Palaearctic region (Huang & Zhang 1999, Dworakowska 2002). The subgenus Zhihadina includes only 1 species from China (Yang & Lee, 1991). So far, four species of subgenus Eurhadina were recorded in the Korean Peninsula (Kwon & Huh 2001): Eurhadina (Eurhadina) betularia Anufriev, 1969, E. (E.) koreana Dworakowska, 1971, E. (E.) pulchella (Fallen, 1806), and E. (E.) wagneri Dworakowska, 1969. The majority of species belonging to the subgenus Eurhadina are difficult to distinguish by external appearance because the color patterns of the forewings are very similar among species.

  1. First Findings in the Route of the Maize Bushy Stunt Phytoplasma Within Its Vector Dalbulus maidis (Hemiptera: Cicadellidae).

    Science.gov (United States)

    González, J García; Ossamu Tanaka, F A; Spotti Lopes, J R

    2016-04-01

    In the pathosystem of Dalbulus madis (DeLong & Wolcott) (Hemiptera: Cicadellidae), a vector of maize bushy stunt phytoplasma (MBSP), the interactions occurring during the passage, invasion, and multiplication of the phytoplasma inside the vector body have been generalized from other pathosystems, with a poor understanding of the specific interactions. With the aim to understand MBSP movement and potential specific interactions with its vector, D. maidis adults were dissected to obtain the intestine and salivary gland of both infected (acquisition access period=4 d; latent period=23 d) and noninfected individuals. The organs were processed for visualization with transmission electronic microscopy. Images of phytoplasma cells were observed in the alimentary canal, epithelium of the mesenteron, hemocele, and salivary gland of the vector, and were confirmed through observation of similar cells in maize roots with advanced disease symptoms. The study of the MBSP movement within its vector shows novel findings between the synergy of the MBSP phytoplasma and D. maidis.

  2. Predation potential of Chilocorus cacti (Coleoptera: Coccinellidae) to the prickly pear cacti pest Dactylopius opuntiae (Hemiptera: Dactylopiidae).

    Science.gov (United States)

    Flores, A; Olvera, H; Rodríguez, S; Barranco, J

    2013-08-01

    Functional response of the predator Chilocorus cacti (Linnaeus) (Coleoptera: Coccinellidae) on five densities of Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) female adults was assessed under laboratory conditions. The searching efficiency of C. cacti significantly decreased as prey density increased. The logistic regression for the predator had a negative and significant linear parameter indicating a type II functional response. Non-linear regression for Holling predator equation estimated a handling time of 1.79 ± 0.129 h and attack rate coefficient of 0.1003 ± 0.030. Most of this handling time was because the predator spent a lot of time removing the waxy coating that protects adult females of D. opuntiae. Chilocorus cacti consumes females of D. opuntiae in their reproductive stage; therefore, it could be an effective natural enemy to suppress or regulate low density populations of D. opuntiae, preventing them to reach high densities.

  3. Host plant preference of harlequin bug (Hemiptera: Pentatomidae), and evaluation of a trap cropping strategy for its control in collard.

    Science.gov (United States)

    Wallingford, Anna K; Kuhar, Thomas P; Pfeiffer, Douglas G; Tholl, Dorothea B; Freeman, Joshua H; Doughty, Hélène B; Schultz, Peter B

    2013-02-01

    Harlequin bug, Murgantia histrionica (Hahn) (Hemiptera: Pentatomidae), is a piercing-sucking pest of cole crops, causing cosmetic damage in low populations, while heavy pest pressure can kill plants or entire fields. Field studies were conducted to evaluate a trap crop for control of harlequin bug in collard. Field-cage choice tests found that potential trap crop plant species, mustard (Brassica juncea 'Southern Giant Curled'), rapeseed (B. napus 'Athena'), rapini (B. rapa), and arugula (Eruca satica) attracted more harlequin bugs than collard (B. oleracea 'Champion') and a nonbrassica control, bean (Phaseolus vulgaris'Bronco'). Mustard was the most consistently selected by harlequin bug over collard in choice tests, and was found to be an effective trap crop for reducing feeding injury on collard at two experimental sites. Augmentation of the mustard trap crop with a systemic, neonicotinoid insecticide provided no added control of harlequin bug for the 10 wk duration in the spring season.

  4. Morphology of the European species of the aphid genus Eulachnus (Hemiptera: Aphididae: Lachninae) - A SEM comparative and integrative study.

    Science.gov (United States)

    Kanturski, Mariusz; Karcz, Jagna; Wieczorek, Karina

    2015-09-01

    Scanning electron microscopy (SEM) methods were used for the first time to elucidate the external morphology of the European species of the genus Eulachnus (Hemiptera: Aphididae: Lachninae), a representative genus of the conifer-feeding aphids tribe Eulachnini. We examined and compared the external morphology of apterous and alate viviparous females from the parthenogenetic generation as well as oviparous females and alate males belonging to the sexual generation. FE-SEM images based on HMDS and cryo-SEM preparation techniques revealed better image quality than the CPD technique in regard to surface tension and morphological signs of cell deteriorations (i.e., existence of depressions, drying artifacts and membrane blebs). Three morphologically different species groups "agilis", "brevipilosus" and "cembrae" were proposed due to the differences in head, antennae, legs and dorsal chaetotaxy as well as dorsal sclerotization. The most characteristic features and differences of representatives of these groups are presented and discussed.

  5. Nuclear rDNA-based molecular clock of the evolution of triatominae (Hemiptera: Reduviidae, vectors of Chagas disease

    Directory of Open Access Journals (Sweden)

    Bargues MD

    2000-01-01

    Full Text Available The evolutionary history and times of divergence of triatomine bug lineages are estimated from molecular clocks inferred from nucleotide sequences of the small subunit SSU (18S and the second internal transcribed spacer (ITS-2 of the nuclear ribosomal DNA of these reduviids. The 18S rDNA molecular clock rate in Triatominae, and Prosorrhynchan Hemiptera in general, appears to be of 1.8% per 100 million years (my. The ITS-2 molecular clock rate in Triatominae is estimated to be around 0.4-1% per 1 my, indicating that ITS-2 evolves 23-55 times faster than 18S rDNA. Inferred chronological data about the evolution of Triatominae fit well with current hypotheses on their evolutionary histories, but suggest reconsideration of the current taxonomy of North American species complexes.

  6. Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

    NARCIS (Netherlands)

    Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

    2011-01-01

    On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T)  = JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T)  = JCM 1

  7. Descrição de um gênero novo e cinco espécies novas de Neocoelidiinae (Hemiptera, Auchenorrhyncha, Cicadellidae Description of a new genus and five new species of Neocoelidiinae (Hemiptera, Auchenorrhyncha, Cicadellidae

    Directory of Open Access Journals (Sweden)

    Ana Paula Marques-Costa

    2007-01-01

    Full Text Available Um gênero novo de Neocoelidiinae, Paracoelidiana gen. nov., e cinco espécies novas - Paracoelidiana hastata sp. nov., Paracoelidiana lamellata sp. nov., Paracoelidiana longipenis sp. nov., Paracoelidiana morretensis sp. nov. e Paracoelidiana recifensis sp. nov. - são descritos. Esse gênero novo é próximo a Coelidana Oman, 1936, apresentando morfologia externa muito semelhante, sua separação sendo feita apenas com base na morfologia da genitália masculina. As fêmeas do gênero novo não são conhecidas. Todas as espécies são ilustradas, uma chave para identificação das mesmas e sua distribuição geográfica conhecida são fornecidas.A new genus of Neocoelidiinae, Paracoelidiana gen. nov., and five new species - Paracoelidiana hastata sp. nov., Paracoelidiana lamellata sp. nov., Paracoelidiana longipenis sp. nov., Paracoelidiana morretensis sp. nov. and Paracoelidiana recifensis sp. nov. - are described. This new genus is similar to Coelidiana Oman, 1936, presenting very similar external morphology, its separation being made based only in the morphology of male genitalia. The females of the new genus are unknown. All the species are illustrated and keyed, and their known geographical distribution is given.

  8. Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line.

    Directory of Open Access Journals (Sweden)

    Georgios Sotiriadis

    Full Text Available Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain on contractile events in vitro, using a human myometrial cell line (ULTR as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR and connexin 43 (CX43. In addition, rhSP-A and rhSP-D were able to induce secretion of GROα and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one.

  9. Comparison of Relative Bias, Precision, and Efficiency of Sampling Methods for Natural Enemies of Soybean Aphid (Hemiptera: Aphididae).

    Science.gov (United States)

    Bannerman, J A; Costamagna, A C; McCornack, B P; Ragsdale, D W

    2015-06-01

    Generalist natural enemies play an important role in controlling soybean aphid, Aphis glycines (Hemiptera: Aphididae), in North America. Several sampling methods are used to monitor natural enemy populations in soybean, but there has been little work investigating their relative bias, precision, and efficiency. We compare five sampling methods: quadrats, whole-plant counts, sweep-netting, walking transects, and yellow sticky cards to determine the most practical methods for sampling the three most prominent species, which included Harmonia axyridis (Pallas), Coccinella septempunctata L. (Coleoptera: Coccinellidae), and Orius insidiosus (Say) (Hemiptera: Anthocoridae). We show an important time by sampling method interaction indicated by diverging community similarities within and between sampling methods as the growing season progressed. Similarly, correlations between sampling methods for the three most abundant species over multiple time periods indicated differences in relative bias between sampling methods and suggests that bias is not consistent throughout the growing season, particularly for sticky cards and whole-plant samples. Furthermore, we show that sticky cards produce strongly biased capture rates relative to the other four sampling methods. Precision and efficiency differed between sampling methods and sticky cards produced the most precise (but highly biased) results for adult natural enemies, while walking transects and whole-plant counts were the most efficient methods for detecting coccinellids and O. insidiosus, respectively. Based on bias, precision, and efficiency considerations, the most practical sampling methods for monitoring in soybean include walking transects for coccinellid detection and whole-plant counts for detection of small predators like O. insidiosus. Sweep-netting and quadrat samples are also useful for some applications, when efficiency is not paramount.

  10. Nymphal and adult performance of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae), as a potential alternative host for egg parasitoids multiplication

    Energy Technology Data Exchange (ETDEWEB)

    Peres, Wilsimar A.A. [Universidade Federal do Parana, Curitiba, PR (Brazil). Dept. de Zoologia; Correa-Ferreira, Beatriz S. [EMBRAPA, Londrina, PR (Brazil). Centro Nacional de Pesquisa de Soja

    2001-12-15

    This research aimed to evaluate the potential of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae) as host for multiplication of egg parasitoids, by determining the nymphal and adult performance of E. heros from laboratory and the field, comparing with Nezara viridula (L.) (Hemiptera: Pentatomidae), under mass conditions. One hundred eggs of E. heros and N. viridula were placed among the leaves of soybean plants contained in cages (50x50x70 cm) and observation were made until adult emergence. The nymphs fed on soybean pods, dry soybean and peanuts seeds. The number of nymphs that reached adulthood and the development time were calculated. The survivorship and reproduction performance of laboratory and field populations of E. heros and N. viridula were evaluated during 13 weeks in February-May 1999. The number of eggs produced by 100 pairs of stink bugs per cage containing the same diet was recorded. Nymphal development time of E. heros and N. viridula was 33.0 and 34.0 days and 65.0% and 71.3% of nymphs reached adulthood, respectively. Adults of E. heros reared under laboratory conditions produced 2.5 times more eggs (5547.0 eggs/cage) than those collected in the field (2262.7 eggs/cage). The adult field population of E. heros had reduced reproduction and longevity due to parasitism by Hexacladia smithii Ash. (Hymenoptera: Encyrtidae). The N. viridula adults collected in the field produced 1.7 times more eggs (6304.9 eggs/cage) than those reared in the laboratory (3609.2 eggs/cage). E. heros laboratory reared is a promising host for egg parasitoids multiplication when compared with N. viridula collected in the field. (author)

  11. Description of Ortheziolamameti tranfagliai new species (Hemiptera, Coccoidea, Ortheziidae from India

    Directory of Open Access Journals (Sweden)

    Éva Szita

    2014-06-01

    Full Text Available This paper describes a new Ortheziolamameti species from the Oriental region (India, namely Ortheziolamameti tranfagliai Konczné Benedicty, sp. n. The examined material was extracted from forest litter from India, using Berlese funnels. With this new species the genus Ortheziolamameti now includes six species. An identification key and distribution map are provided.

  12. Description of Ortheziolamameti tranfagliai new species (Hemiptera, Coccoidea, Ortheziidae) from India.

    Science.gov (United States)

    Szita, Eva; Kaydan, Mehmet Bora; Benedicty, Zsuzsanna Konczné

    2014-01-01

    This paper describes a new Ortheziolamameti species from the Oriental region (India), namely Ortheziolamameti tranfagliai Konczné Benedicty, sp. n. The examined material was extracted from forest litter from India, using Berlese funnels. With this new species the genus Ortheziolamameti now includes six species. An identification key and distribution map are provided.

  13. Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov., isolated from wild Rodentia.

    Science.gov (United States)

    Sato, Shingo; Kabeya, Hidenori; Fujinaga, Yuta; Inoue, Kai; Une, Yumi; Yoshikawa, Yasuhiro; Maruyama, Soichi

    2013-05-01

    Four novel strains of members of the genus Bartonella, OY2-1(T), BR11-1(T), FN15-2(T) and KS2-1(T), were isolated from the blood of wild-captured greater Egyptian jerboa (Jaculus orientalis), plantain squirrel (Callosciurus notatus), fat-tailed gerbil (Pachyuromys duprasi) and golden spiny mouse (Acomys russatus). All the animals were imported to Japan as pets from Egypt, Thailand and the Netherlands. The phenotypic characterization (growth conditions, incubation periods, biochemical properties and cell morphologies), DNA G+C contents (37.4 mol% for strain OY2-1(T), 35.5 mol% for strain BR11-1(T), 35.7 mol% for strain FN15-2(T) and 37.2 mol% for strain KS2-1(T)), and sequence analyses of the 16S rRNA genes indicated that those strains belong to the genus Bartonella. Sequence comparisons of gltA and rpoB genes suggested that all of the strains should be classified as novel species of the genus Bartonella. In phylogenetic trees based on the concatenated sequences of five loci, including the 16S rRNA, ftsZ, gltA and rpoB genes and the ITS region, and on the concatenated deduced amino acid sequences of three housekeeping genes (ftsZ, gltA and rpoB), all strains formed distinct clades and had unique mammalian hosts that could be discriminated from other known species of the genus Bartonella. These data strongly support the hypothesis that strains OY2-1(T), BR11-1(T), FN15-2(T) and KS2-1(T) should be classified as representing novel species of the genus Bartonella. The names Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov. are proposed for these novel species. Type strains of Bartonella jaculi sp. nov., Bartonella callosciuri sp. nov., Bartonella pachyuromydis sp. nov. and Bartonella acomydis sp. nov. are OY2-1(T) ( = JCM 17712(T) = KCTC 23655(T)), BR11-1(T) ( = JCM 17709(T) = KCTC 23909(T)), FN15-2(T) ( = JCM 17714(T) = KCTC 23657(T)) and KS2-1(T) ( = JCM 17706(T

  14. Review of the genus Arhaphe (Hemiptera: Heteroptera: Largidae) with descriptions of nine new species from Central America.

    Science.gov (United States)

    Stehlík, Jaroslav L; Brailovsky, Harry

    2016-03-24

    Nine new species of the genus Arhaphe Herrich-Schaeffer, 1850 are described: A. ferruginea sp. nov. from Mexico (Guerrero), A. flavoantennata sp. nov. from Costa Rica (Guanacaste Province), Honduras (Intibuca Department) and Nicaragua (Granada Province), A. hirsuta sp. nov. from Mexico (Oaxaca), A. kmenti sp. nov. from Mexico (Guanajuato, Michoacán), A. longula sp. nov. from Mexico (Guerrero), A. magna sp. nov. from Mexico (Colima), A. myrmicoides sp. nov. from Mexico (Guerrero, Nayarit), A. oaxacana sp. nov. from Mexico (Oaxaca), and A. pilifera sp. nov. from Mexico (Nayarit). The fifth instar larvae of A. arguta (Bliven, 1956), A. carolina Herrich-Schaeffer, 1850, A. cicindeloides Walker, 1873, A. flavoantennata sp. nov., A. mexicana Halstead, 1972, and A. morelensis Brailovsky & Marquez, 1974 are described. Additional state faunistic records of the already described species are provided: A. arguta (Bliven, 1956) (Mexico: Guanajuato, Sonora), A. capitata Halstead, 1972 (Costa Rica; Mexico: Chiapas, Estado de México, Guerrero, Michoacán, Oaxaca, Puebla), A. carolina Herrich-Schaeffer, 1850 (Mexico: Guanajuato, Tamaulipas), A. cicindeloides Walker, 1873 (Mexico: Hidalgo, Querétaro), A. deviatica Brailovsky, 1981 (Nicaragua), A. furcata Brailovsky, 1981 (Mexico: Michoacán), A. halsteadi Brailovsky, 1981 (Mexico: Oaxaca), A. mimetica Barber, 1911 (Mexico: Chihuahua), A. morelensis Brailovsky & Marquez, 1974 (Mexico: Estado de México, Guerrero, Puebla), and A. rustica Brailovsky, 1981 (Mexico: Morelos, Oaxaca).

  15. Temperature-Dependent Fecundity and Life Table of the Fennel Aphid Hyadaphis foeniculi (Passerini (Hemiptera: Aphididae.

    Directory of Open Access Journals (Sweden)

    Francisco S Ramalho

    Full Text Available Hyadaphis foeniculi (Passerini (Hemiptera: Aphididae is a cosmopolitan species and the main pest of fennel in northeastern Brazil. Understanding the relationship between temperature variations and the population growth rates of H. foeniculi is essential to predict the population dynamics of this aphid in the fennel crop. The aim of this study was to measure the effect of constant temperature on the adult prereproductive period and the life table fertility parameters (infinitesimal increase ratio (rm, gross reproduction rate (GRR, net reproduction rate (R0, finite increase ratio (λ, generation time (GT, the time required for the population to double in the number of individuals (DT, and the reproduction value (RVx of the fennel pest H. foeniculi. The values of lx (survival of nymphs at age x increased as the temperature rose from 15 to 28°C and fell at 30°C, whereas mx (number of nymphs produced by each nymph of age x increased from 15 to 25°C and fell at 28 and 30°C. The net reproduction rates (R0 of populations of H. foeniculi increased with temperature and ranged from 1.9 at 15°C to 12.23 at 28°C for each generation. The highest population increase occurred with the apterous aphids at 28°C. The rate of population increase per unit time (rm (day ranged from 0.0033 (15°C to 0.1995 (28°C. The highest values of rm were recorded at temperatures of 28°C and 30°C. The rm values were a good fit to the models tested, with R2 > 0.91 and R2adj > 0.88. The models tested (Davidson, Sharpe and DeMichele modified by Schoolfield et al., Logan et al., Lamb, and Briere et al. were very good fits for the rm values observed, with R2 > 0.91 and R2adj > 0.88. The only exception was the Davidson model. Of the parameters studied, the reproductive capacity was higher in the apterous aphids, with the unique exception of daily fecundity at 28°C, which was higher in the alate aphids of H. foeniculi. Parameters relating to the age-specific fertility table for

  16. Inventory and assessment of foliar natural enemies of the soybean aphid (Hemiptera: Aphididae) in South Dakota.

    Science.gov (United States)

    Hesler, Louis S

    2014-06-01

    Soybean aphid (Aphis glycines Matsumura) (Hemiptera: Aphididae) is a major pest of soybean in northern production regions of North America, and insecticides have been the primary management approach while alternative methods are developed. Knowledge of arthropod natural enemies and their impact on soybean aphid is critical for developing biological control as a management tool. Soybean is a major field crop in South Dakota, but information about its natural enemies and their impact on soybean aphid is lacking. Thus, this study was conducted in field plots in eastern South Dakota during July and August of 2004 and 2005 to characterize foliar-dwelling, arthropod natural enemies of soybean aphid, and it used exclusion techniques to determine impact of natural enemies and ants (Hymenoptera: Formicidae) on soybean aphid densities. In open field plots, weekly soybean aphid densities reached a plateau of several hundred aphids per plant in 2004, and peaked at roughly 400 aphids per plant in 2005. Despite these densities, a relatively high frequency of aphid-infested plants lacked arthropod natural enemies. Lady beetles (Coleoptera: Coccinellidae) were most abundant, peaking at 90 and 52% of all natural enemies sampled in respective years, and Harmonia axyridis Pallas was the most abundant lady beetle. Green lacewings (Neuroptera: Chrysopidae) were abundant in 2005, due mainly to large numbers of their eggs. Abundances of arachnids and coccinellid larvae correlated with soybean aphid densities each year, and chrysopid egg abundance was correlated with aphid density in 2005. Three-week cage treatments of artificially infested soybean plants in 2004 showed that noncaged plants had fewer soybean aphids than caged plants, but abundance of soybean aphid did not differ among open cages and ones that provided partial or total exclusion of natural enemies. In 2005, plants within open cages had fewer soybean aphids than those within cages that excluded natural enemies, and aphid

  17. Temperature-Dependent Fecundity and Life Table of the Fennel Aphid Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae).

    Science.gov (United States)

    Ramalho, Francisco S; Malaquias, José B; Lira, Aline C S; Oliveira, Flávia Q; Zanuncio, José C; Fernandes, Francisco S

    2015-01-01

    Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae) is a cosmopolitan species and the main pest of fennel in northeastern Brazil. Understanding the relationship between temperature variations and the population growth rates of H. foeniculi is essential to predict the population dynamics of this aphid in the fennel crop. The aim of this study was to measure the effect of constant temperature on the adult prereproductive period and the life table fertility parameters (infinitesimal increase ratio (rm), gross reproduction rate (GRR), net reproduction rate (R0), finite increase ratio (λ), generation time (GT), the time required for the population to double in the number of individuals (DT), and the reproduction value (RVx)) of the fennel pest H. foeniculi. The values of lx (survival of nymphs at age x) increased as the temperature rose from 15 to 28°C and fell at 30°C, whereas mx (number of nymphs produced by each nymph of age x) increased from 15 to 25°C and fell at 28 and 30°C. The net reproduction rates (R0) of populations of H. foeniculi increased with temperature and ranged from 1.9 at 15°C to 12.23 at 28°C for each generation. The highest population increase occurred with the apterous aphids at 28°C. The rate of population increase per unit time (rm) (day) ranged from 0.0033 (15°C) to 0.1995 (28°C). The highest values of rm were recorded at temperatures of 28°C and 30°C. The rm values were a good fit to the models tested, with R2 > 0.91 and R2adj > 0.88. The models tested (Davidson, Sharpe and DeMichele modified by Schoolfield et al., Logan et al., Lamb, and Briere et al.) were very good fits for the rm values observed, with R2 > 0.91 and R2adj > 0.88. The only exception was the Davidson model. Of the parameters studied, the reproductive capacity was higher in the apterous aphids, with the unique exception of daily fecundity at 28°C, which was higher in the alate aphids of H. foeniculi. Parameters relating to the age-specific fertility table for H

  18. Distribution of Bemisia tabaci (Hemiptera: Aleyrodidae) biotypes in North America after the Q invasion.

    Science.gov (United States)

    McKenzie, Cindy L; Bethke, James A; Byrne, Frank J; Chamberlin, Joseph R; Dennehy, Timothy J; Dickey, Aaron M; Gilrein, Dan; Hall, Paula M; Ludwig, Scott; Oetting, Ronald D; Osborne, Lance S; Schmale, Lin; Shatters, Robert G

    2012-06-01

    After the 2004 discovery of the Bemisia tabaci (Gennadius) (Hemiptera Aleyrodidae) Q biotype in the United States, there was a vital need to determine the geographical and host distribution as well as its interaction with the resident B biotype because of its innate ability to rapidly develop high-level insecticide resistance that persists in the absence of exposure. As part of a coordinated country-wide effort, an extensive survey of B. tabaci biotypes was conducted in North America, with the cooperation of growers, industry, local, state, and federal agencies, to monitor the introduction and distribution of the Q biotype. The biotype status of submitted B. tabaci samples was determined either by polymerase chain reaction amplification and sequencing of a mitochondrial cytochrome oxidase I small subunit gene fragment and characterization of two biotype discriminating nuclear microsatellite markers or esterase zymogram analysis. Two hundred and eighty collections were sampled from the United States, Bermuda, Canada, and Mexico during January 2005 through December 2011. Host plants were split between ornamental plant and culinary herb (67%) and vegetable and field crop (33%) commodities. The New World biotype was detected on field-grown tomatoes (Solanum lycopersicum L.) in Mexico (two) and in commercial greenhouses in Texas (three) and represented 100% of these five collections. To our knowledge, the latter identification represents the first report of the New World biotype in the United States since its rapid displacement in the late 1980s after the introduction of biotype B. Seventy-one percent of all collections contained at least one biotype B individual, and 53% of all collections contained only biotype B whiteflies. Biotype Q was detected in 23 states in the United States, Canada (British Columbia and Ontario territories), Bermuda, and Mexico. Forty-five percent of all collections were found to contain biotype Q in samples from ornamentals, herbs and a single

  19. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  20. O gênero Garapita Oman (Hemiptera, Cicadellidae, Deltocephalinae com descrições de quatro novas espécies The genus Garapita Oman (Hemiptera, Cicadellidae, Deltocephalinae with descriptions of four new species

    Directory of Open Access Journals (Sweden)

    Keti Maria Rocha Zanol

    2003-06-01

    Full Text Available Garapita Oman is redescribed and four new species are described, G. (G. guajarensis sp. nov., G. (G. sinopia sp. nov., G. (G. computa sp. nov., from Brazil and G. (G. paraguaiensis sp. nov. from Paraguay. A new combination is established, G. (G. clitellaria (Osborn, 1923, comb. nov. and G. (G. pulchripennis Linnavuori, 1959 is considered a new synonym. The male of G. (G. clitellaria, up to now unknown, is described. G. (G. garbosa Oman, 1936 is reported for the first time in Brazil; G. (Chlamydopita aurea Linnavuori, 1959 is redescribed and illustrated.

  1. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  2. Som man spørger

    DEFF Research Database (Denmark)

    Bjerg, Lars

    Indhold: Input og outputinterview ; Tre interviewtyper ; Tre grundprincipper ; De syv dødssynder ; Åbne, simple og neutrale spørgsmål ; Skuffespørgsmål ; Fokus ; Aktiv lytning ; Mål ; Strategi ; Abstrakt og konkret ; Det kritiske interview ; Kilder med et budskab ; Oldfruens fif ; Resumé i punktform...

  3. A new virginae butanolide from Streptomyces sp.

    Institute of Scientific and Technical Information of China (English)

    Xiang LI; Yi Nan ZHENG; Wen Han LIN; Isabel SATTLER

    2006-01-01

    A novel butanolide, named virginaebutanolide F (1), was isolated from the lyophilized culture broth of Streptomyces sp., along with a known compound virginaebutanolide C (2). Their structures including the stereochemistry were elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS and CD spectroscopic analysis.

  4. Framework, The Physics of sp(2,R)

    CERN Document Server

    Hawthorn, Ian; Ussher, Matthew

    2015-01-01

    A mathematical framework for relativistic quantum field theory is constructed with natural symmetry $\\mathsf{so}(2,3)= \\mathsf{sp}(2,\\mathbb{ R})$. In this framework gravity and electromagnetism unify as aspects of the geometry. The source equation for gravity differs from Einstein's equation and permits behaviour that could explain dark matter.

  5. Carbon sp chains in graphene nanoholes

    DEFF Research Database (Denmark)

    Castelli, Ivano Eligio; Ferri, Nicola; Onida, Giovanni

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We...

  6. Updated check-list of iberian-balearic Aphidini (Hemiptera, Aphididae

    Directory of Open Access Journals (Sweden)

    García Prieto, Francisca

    2004-12-01

    Full Text Available The check-list of species in the Tribe Aphidini (Hemiptera, Aphididae: Aphidinae recorded to date from the Iberian Peninsula and Balearic Isles is presented, 139 species are listed. A list of the countries (Andorra, Spain and Portugal and provinces (Spanish or districts (Portuguese where each species and subspecies is known is also included. Five species are mentioned for the first time in Iberian-Balearic territory: Aphis callunae Theobald, A. comosa (Börner, A. lantanae Koch (with the subspecies A. lantanae coriaria Börner, A. erigerontis Holman and Schizaphis longicaudata Hille Ris Lambers. Nine species have been removed from the check-list: Aphis euphorbiae Kaltenbach, A. genistae Scopoli, A. pilosellae (Börner, A. salsolae (Börner, A. striata Hille Ris Lambers, Brachyunguis zygophylli (Nevsky, B. suaedus (Paik, Protaphis centaurea (Gómez-Menor and Schizaphis pilipes (Ossiannilsson. Aphis ruborum Börner & Schilder, 1931 is recognized as the valid name for Aphis ruborum Börner, 1932 syn. nov. and Rhopalosiphum oxyacanthae (Schrank for Rhopalosiphum insertum (Walker. Two new combinations are established: Xerobion blascoi comb. nov. for Aphis blascoi García Prieto & Sanchís Segovia and Xerobion brutii comb. nov. for Absinthaphis brutii Barbagallo. Five binomens have been re-established: A. althaeae (Nevsky, A. ballotae Passerini, A. longirostris (Börner, A. ononidis (Schouteden and A. picridis (Börner to replace A. davletshinae Hille Ris Lambers, A. balloticola Szelegiewicz, A. longirostrata Hille Ris Lambers, A. kaltenbachi Hille Ris Lambers and A. stroyani Szelegiewicz, respectively. The life cycle of Aphis stachydis Mordvilko is given and its males and oviparous females are described. Taxonomic

  7. Distribuição espacial de Aphis gossypii (Glover (Hemiptera, Aphididae e Bemisia tabaci (Gennadius biótipo B (Hemiptera, Aleyrodidae em algodoeiro Bt e não-Bt Spatial distribution of Aphis gossypii (Glover (Hemiptera, Aphididae and Bemisia tabaci (Gennadius biotype B (Hemiptera, Aleyrodidae on Bt and non-Bt cotton

    Directory of Open Access Journals (Sweden)

    Tatiana Rojas Rodrigues

    2010-03-01

    Full Text Available Distribuição espacial de Aphis gossypii (Glover (Hemiptera, Aphididae e Bemisia tabaci (Gennadius biótipo B (Hemiptera, Aleyrodidae em algodoeiro Bt e não-Bt. O estudo da distribuição espacial de adultos de Bemisia tabaci e de Aphis gossypii nas culturas do algodoeiro Bt e não-Bt é fundamental para a otimização de técnicas de amostragens, além de revelar diferenças de comportamento de espécies não-alvo dessa tecnologia Bt entre as duas cultivares. Nesse sentido, o experimento buscou investigar o padrão da distribuição espacial dessas espécies de insetos no algodoeiro convencional não-Bt e no cultivar Bt. As avaliações ocorreram em dois campos de 5.000 m² cada, nos quais se realizou 14 avaliações com contagem de adultos da mosca-branca e colônias de pulgões. Foram calculados os índices de agregação (razão variância/média, índice de Morisita e Expoente k da Distribuição Binomial Negativa e realizados os testes ajustes das classes numéricas de indivíduos encontradas e esperadas às distribuições teóricas de freqüência (Poisson, Binomial Negativa e Binomial Positiva. Todas as análises mostraram que, em ambas as cultivares, a distribuição espacial de B. tabaci ajustou-se a distribuição binomial negativa durante todo o período analisado, indicando que a cultivar transgênica não influenciou o padrão de distribuição agregada desse inseto. Já com relação às análises para A. gossypii, os índices de agregação apontaram distribuição agregada nas duas cultivares, mas as distribuições de freqüência permitiram concluir a ocorrência de distribuição agregada apenas no algodoeiro convencional, pois não houve nenhum ajuste para os dados na cultivar Bt. Isso indica que o algodão Bt alterou o padrão normal de dispersão dos pulgões no cultivo.The study of spatial distribution of the adults of Bemisia tabaci and the colonies of Aphis gossypii on Bt and non-Bt cotton crop is fundamental for

  8. Candida flosculorum sp. nov. and Candida floris sp. nov., two yeast species associated with tropical flowers.

    Science.gov (United States)

    Rosa, Carlos A; Pagnocca, Fernando C; Lachance, Marc-André; Ruivo, Carla C C; Medeiros, Adriana O; Pimentel, Mariana R C; Fontenelle, Julio C R; Martins, Rogério P

    2007-12-01

    Two ascomycetous yeast species, Candida flosculorum sp. nov. and Candida floris sp. nov., were isolated from tropical flowers and their associated insects. C. flosculorum was isolated from flower bracts of Heliconia velloziana and Heliconia episcopalis (Heliconiaceae) collected from two Atlantic rain forest sites in Brazil. C. floris was isolated from flowers of Ipomoea sp. (Convolvulaceae) growing on the banks of the river Paraguai in the pantanal ecosystem in Brazil and from an adult of the stingless bee Trigona sp. and a flower of Merremia quinquefolia (Convolvulaceae) in Costa Rica. C. flosculorum belongs to the Metschnikowiaceae clade and C. floris belongs to the Starmerella clade. The type strain of C. flosculorum is UFMG-JL13(T) (=CBS 10566(T)=NRRL Y-48258(T)) and the type strain of C. floris is UWO(PS) 00-226.2(T) (=CBS 10593(T)=NRRL Y-48255(T)).

  9. Cultivation of Microalgae Chlorella sp. and Scenedesmus sp. as a Potentional Biofuel Feedstock

    Directory of Open Access Journals (Sweden)

    Prof. dr. Violeta Makareviciene

    2011-10-01

    Full Text Available The growth of two robust algae strains Chlorella sp. and Scenedesmus sp. growing in Lithuanian lakes was investigated with the aim to obtain optimum conditions for biomass cultivation for biofuel production in the Lithuanian environment. Samples were taken from different nitrogen sources and of different concentrations, with addition of various concentrations of CO2 and in the presence of salt. The best biomass productivity was achieved using urea as a nitrogen source or modified growing medium BG11 with decreased concentration of NaNO3. The positive impact on the growth of biomass was achieved by aeration with CO2 (especially with concentration of 24%. Additional research into the removal of pollutants, such inorganic salts of nitrogen and phosphorus and organic materials from wastewater using microalgae has revealed good possibilities of using both algae strains in wastewater treatment plants. A content of oil in Chlorella sp. and Scenedesmus sp. has suggested their potential use as biodiesel feedstock.

  10. Nanocrystalline sp 2 and sp 3 carbons: CVD synthesis and applications

    Science.gov (United States)

    Terranova, M. L.; Rossi, M.; Tamburri, E.

    2016-11-01

    The design and production of innovative materials based on nanocrystalline sp 2- and sp 3-coordinated carbons is presently a focus of the scientific community. We present a review of the nanostructures obtained in our labs using a series of synthetic routes, which make use of chemical vapor deposition (CVD) techniques for the selective production of non-planar graphitic nanostructures, nanocrystalline diamonds, and hybrid two-phase nanostructures.

  11. KEMAMPUAN HUTAN MANGROVE RUMPUN RHIZHOPHORA SP DAN AVICENNIA SP DALAM MEREDAM GELOMBANG LAUT

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    A. Kristiyanto

    2014-08-01

    Full Text Available ABSTRAKProses abrasi yang terjadi di wilayah pantai dapat dicegah dengan meredam gelombang laut menggunakan vegetasi mangrove. Tingkat kemampuan vegetasi mangrove dalam meredam gelombang dapat ditentukan dengan mengetahui karakteristik gelombang laut yang menuju garispantai. Pelaksanaan penelitian ini diawalidengan survei lokasi pada mangrove rumpun rhizophora sp dan avicennia sp yang memiliki dasar pantai yang landaidanfluktuasi pasang surut yang cukup tinggi.Kemudian pada area tersebut dibuat kanal dengan ukuran 30 x 6,5 x 1 meter, selanjutnya dihitung porositasnya melalui pengukuran diameter, panjang akar dan batang pohon yang terendam air laut. Pengukuran tinggi dan periode gelombang datang serta gelombang yang ditransmisikan dengan alat ukur SBE (Sea Bird Electronic pada jarak dan porositas tertentu. Hasil penelitian menunjukkan bahwa padarumpun Rhizophora sp dengan tingkat porositas sebesar 0,9828 memiliki kemampuan meredam gelombang laut  sebesar 57,73 %,sedangkan pada rumpun Avicennia sp dengan tingkat porositas 0,9941menghasilkan redaman gelombang laut sebesar 39,60 %. ABSTRACTAbrasion process that occurs at the coastal areas can be prevented by minimizing the sea wave  using mangrove vegetation.The ability of mangrove vegetation in minimizing the wave can be determined bystudying  the sea wave characteristic approaching to the coastal line. The observation starts by finding the location of Rhizophora sp class and Avicennia sp class which have flat coastal areas with high tide fluctuation.The canal with size of 30 x 6,5 x 1 meter were made on the area.The diameter and length of tree roots as well assubmerged trunk were measured to define the porosity. The height and period of both incoming wave and transmited wave were recorded by SBE (Sea Bird Electronic in a particular distance and porosity. The result of observation showed that on porosity of 0.9828 the ability in minimizing sea wave value of rhizopora sp class is 57.73 %, while

  12. Two new species of Sundarion (Hemiptera: Membracidae: Darninae and taxonomic notes on two congeneric taxa

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    Antonio J. Creão-Duarte

    2016-01-01

    Full Text Available ABSTRACT Two new species of Sundarion Kirkaldy, 1904 - Sundarion flavopiceum sp. nov. (from Brazil, state of Pará, Serra Norte and Sundarion marmoratum sp. nov. (from French Guiana, Montagne des Chevaux - are described. The first species differs from S. flavum (Fairmaire, 1846 in having smaller supra-humeral horns, and being dark brown with a large yellow spot on each side of the posterior process. In this aspect, it resembles Alcmeone picea (Fairmaire, 1846. The second species is similar to S. flavum except for being variegate yellowish-brown. Taxonomic comments are provided for S. notabile Souza & Rothéa, 2005, which was previously known only from males, and for S. compressicornis (Fairmaire, 1846 comb. nov., originally described in Hemiptycha Germar, 1833 and latter transferred to Hemikyptha Metcalf, 1927. New geographical distribution records are provided for both species.

  13. Description of a second species of Angucephala DeLong & Freytag (Hemiptera: Cicadellidae: Iassinae: Gyponini

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    Clayton Corrêa Gonçalves

    2015-12-01

    Full Text Available ABSTRACT A second species of Angucephala DeLong & Freytag, 1975 is described and illustrated from Ecuador, A. freytagi sp. nov. (Napo Province. This species can be distinguished from the type species (A. mellana DeLong & Freytag, 1975 mainly by features of the male pygofer and styles. A redescription of the genus and illustrations of the type species are also provided.

  14. Review of the idiocerine leafhoppers of Pakistan (Hemiptera, Cicadellidae) with a description of a new species.

    Science.gov (United States)

    Khatri, Imran; Webb, M D

    2014-01-01

    The Idiocerinae of Pakistan are reviewed and a new species, Tasnimocerus sindhensis sp. nov. (Pakistan: Tandojam), is described and illustrated from Pakistan. Two new junior synonyms of Idioscopus nitidulus (Walker) are recognized: Idioscopus karachiensis Ahmed, Naheed & Ahmed syn. nov. and I. freytagi Ahmed, Naheed & Ahmed syn. nov. Idioscopus nagpurensis (Pruthi) is newly recorded from Pakistan. A checklist of Idiocerinae from Pakistan is also provided together with a key to genera and species. 

  15. First records of Acizzia jamatonica (Kuwayama) and Glycaspis brimblecombei Moore, (Hemiptera: Psyllidae, Aphalaridae) in Montenegro

    OpenAIRE

    Malumphy, Chris; Perović, Tatjana; Hrnčić, Snježana; RADONJIĆ, SANJA; RAIČEVIĆ, MILORAD

    2013-01-01

    Albizia psyllid, Acizzia jamatonica (Kuwayama), and red gum lerp psyllid, Glycaspis brimblecombei Moore, were found in Montenegro for the first time during 2009 and 2012 respectively. Acizzia jamatonica is native to East Asia, and has spread rapidly in Europe since it was first reported from Italy in 2002. It was first collected from Albizzia julibrissin in Podgorica, September 2009, and subsequently from Albizzia sp. and A. julibrissin in Herceg Novi, October 2010, and June and October 2012,...

  16. A new genus and species of Macrosiphini (Hemiptera, Aphididae from China, living on Isodon eriocalyx

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    Bin Zhang

    2013-12-01

    Full Text Available The aphid genus Nigritergaphis gen. n. is described, and N. crassisetosa sp.n. on Isodon eriocalyx (Dunn Kudô (Lamiaceae from Yunnan, China is described and illustrated. The new species curls and distorts the leaves of this important traditional Chinese medicinal plant, and is evidently specific to this host. Holotype and paratypes are deposited in the National Zoological Museum of China, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

  17. Gadigaleyrodes froggatti, a new genus and species of whitefly (Hemiptera: Aleyrodidae) from Australia.

    Science.gov (United States)

    Dooley, John W; Gillespie, Peter

    2013-01-17

    A new monotypic genus of whiteflies (Aleyrodidae), Gadigaleyrodes gen.n., is described and illustrated for G. froggatti sp.n. from New South Wales, Australia. Specimens were collected by W. W. Froggatt in 1899 on Syncarpia glomulifera, and subsequently by P. S. Gillespie on an unknown climbing plant. The genus has unusual morphological features with traits common to both Aleurodicinae and Aleyrodinae. The subfamily placement is discussed, and a key provided to discriminate this taxon from similar whitefly genera in Australia.

  18. Isolation and identification of culturable bacteria from honeydew of whitefly, Bemisia tabaci (G. (Hemiptera: Aleyrodidae

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    Roopa H.K.

    2014-12-01

    Full Text Available Bemisia tabaci (G. is an important pest and a vector of Gemini viruses infecting plants. During the process of feeding B. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. Recent report proved that volatile emitted by the honeydew associated bacteria of aphid, Acyrthosiphon pisum Harris was involved in natural enemy calling. Thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest management. In this perspective, very less information is available on bacteria associated with the honeydew excreted by B. tabaci. Therefore, in the present study we have isolated and characterized three culturable bacteria from the honeydew of B. tabaci viz. Bacillus endophyticus, Bacillus niacini and Roseomonas species by employing 16Sr DNA BLASTx analyses which revealed that both B. endophyticus and B. niacini had high similarity (>99% to the respective species, while Roseomonas sp. showed only 95% similarity to the existing Roseomonas sp. specificity of honeydew association of Roseomonas sp. was confirmed by developing specific primers as this genus is reported from immunocompromised persons and recently from ticks and mites. The present study also indicated the possible host-plant origin of these honeydew associated bacteria.

  19. Isolation and identification of culturable bacteria from honeydew of whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    H K, Roopa; K B, Rebijith; R, Asokan; Mahmood, Riaz; N K, Krishna Kumar

    2014-12-01

    Bemisia tabaci (G.) is an important pest and a vector of Gemini viruses infecting plants. During the process of feeding B. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. Recent report proved that volatile emitted by the honeydew associated bacteria of aphid, Acyrthosiphon pisum Harris was involved in natural enemy calling. Thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest management. In this perspective, very less information is available on bacteria associated with the honeydew excreted by B. tabaci. Therefore, in the present study we have isolated and characterized three culturable bacteria from the honeydew of B. tabaci viz. Bacillus endophyticus, Bacillus niacini and Roseomonas species by employing 16Sr DNA BLASTx analyses which revealed that both B. endophyticus and B. niacini had high similarity (> 99%) to the respective species, while Roseomonas sp. showed only 95% similarity to the existing Roseomonas sp. specificity of honeydew association of Roseomonas sp. was confirmed by developing specific primers as this genus is reported from immunocompromised persons and recently from ticks and mites. The present study also indicated the possible host-plant origin of these honeydew associated bacteria.

  20. Mirideos neotropicais, CCCXX: um gênero e duas espécies novos do Brasil (Hemiptera Neotropical miridae: a new genus and two new species from Brazil (Hemiptera

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    José C. M. Carvalho

    1989-01-01

    Full Text Available The authors describe a new genus and two new species of Brazil as follows: Juinacoris n. gen., J. hugoi n. sp., from Juina, Mato Grosso; Perissobasis hugoi n. sp., Juina, Mato Grosso. Figures of habitus and male genitalia are included.

  1. Revalidation of Ceresa terminalis walker and its placement in Stictocephala Stål (Hemiptera, Membracidae Revalidação de Ceresa terminalis walker e sua alocação em Stictocephala Stål (Hemiptera, Membracidae

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    Gabriel S. de Andrade

    2005-12-01

    Full Text Available Ceresa terminalis Walker, 1851 is reinstated and transferred to Stictocephala Stål, 1869: Stictocephala terminalis (Walker, 1851 sp. rev., comb. nov.Ceresa terminalis Walker, 1851 é revalidada e transferida para Stictocephala Stål, 1869: Stictocephala terminalis (Walker, 1851 sp. rev., comb. nov.

  2. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    Energy Technology Data Exchange (ETDEWEB)

    Ilhan-Sungur, Esra, E-mail: esungur@istanbul.edu.tr [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Unsal-Istek, Tuba [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Cansever, Nurhan [Yıldız Technical University, Faculty of Chemistry-Metallurgy, Metallurgical and Materials Engineering Department, 34210 Esenler, Istanbul (Turkey)

    2015-07-15

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel.

  3. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Tatsuo [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women' s University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan); Itoh, Kohji, E-mail: kitoh@ph.tokushima-u.ac.jp [Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, Tokushima 770-8505 (Japan); Kuwahara, Jun, E-mail: jkuwahar@dwc.doshisha.ac.jp [Department of Molecular Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Doshisha Women' s University, Kodo, Kyotanabe City, Kyoto 610-0395 (Japan)

    2010-12-10

    Research highlights: {yields} Sp1 zinc fingers themselves interact with importin {alpha}. {yields} Sp1 zinc finger domains play an essential role as a nuclear localization signal. {yields} Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin {alpha} in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin {alpha} including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin {alpha}. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  4. Revisión de los géneros Eurygerris y Tachygerris (Hemiptera: Tachygerrini para la región neotropical Revision of the genera Eurygerris and Tachygerris (Hemiptera: Tachygerrini from the neotropical region

    Directory of Open Access Journals (Sweden)

    Irina T. Morales-Castaño

    2009-08-01

    Full Text Available Se estudió la taxonomía de la tribu Tachygerrini (Hemiptera: Gerridae: Gerrinae con 2 géneros reconocidos, Eurygerris Hungerford y Matsuda (10 especies y Tachygerris Drake (7 especies, a partir de la revisión de 19 colecciones entomológicas del Neotrópico. El material tipo de las siguientes especies fue revisado: T. opacus, T. quadrilineatus, T. adamsoni, T. dentiferus, E. fuscinervis, E. flavolineatus, E. mexicanus y E. atrekes. Se reconocieron y redescribieron 11 especies de la tribu representadas en el Neotrópico: Tachygerris opacus, T. quadrilineatus, T. celocis, T. adamsoni, T. dentiferus, Eurygerris fuscinervis, E.flavolineatus, E. mexicanus, E. beieri, E. summatis y E. atrekes. Eurygerris kahli (Drake y Harris, 1934 es sinonimizada con Eurygerris fuscinervis (Berg, 1898. Se proporcionan redescripciones, ilustraciones, información nomenclatural y datos sobre la distribución y el hábitat para cada una de las especies. Se amplían los rangos de distribución para T. adamsoni (Venezuela, E. fuscinervis (Guatemala, México y Venezuela y E. atrekes (Venezuela.The taxonomy of the tribe Tachygerrini (Hemiptera: Gerridae: Gerrinae was studied. The tribe comprises 2 genera: Eurygerris Hungerford and Matsuda (with 10 species and Tachygerris Drake (with 7 species. The revision was based on the examination of 19 entomological collections in the neotropics. The type materials of T. opacus, T. quadrilineatus, T. adamsoni, T. dentiferus, E. fuscinervis, E. flavolineatus, E. mexicanus and E. atrekes were reviewed. Eleven species from the tribe were re-described for the Neotropics, Tachygerris opacus, T. quadrilineatus, T. celocis, T. adamsoni, T. dentiferus, Eurygerris fuscinervis, E. flavolineatus, E. mexicanus, E. beieri, E. summatis, and E. atrekes. Eurygerris kahli (Drake y Harris, 1934 is synonymized with Eurygerris fuscinervis (Berg, 1898. Re-descriptions, illustrations, nomenclatural information and distribution and ecological data for

  5. Controle químico da forma galícola da filoxera Daktulosphaira vitifoliae (Fitch, 1856 (Hemiptera: Phylloxeridae na cultura da videira Chemical control of grape phylloxera Daktulosphaira vitifoliae (Fitch, 1856 leaf form (Hemiptera: Phylloxeridae on vineyards

    Directory of Open Access Journals (Sweden)

    Marcos Botton

    2004-10-01

    Full Text Available A filoxera Daktulosphaira vitifoliae (Fitch, 1856 (Hemiptera: Phylloxeridae é considerada a principal praga da videira. O inseto se alimenta da parte aérea e raízes sendo que os maiores prejuízos são observados em raízes de Vitis vinifera cultivada como pé-franco. O dano nas folhas é importante em viveiros, quando o ataque ocorre nos ramos utilizados como porta-enxertos, resistentes à forma radícola. Este trabalho foi realizado com o objetivo de selecionar inseticidas que possam ser empregados como substitutos aos fosforados e piretróides no manejo da forma galícola da filoxera. Os inseticidas imidacloprid, thiamethoxam, acephate, abamectin, deltamethrin e fenitrothion, foram avaliados em condições de campo, sob infestação natural, em plantas matrizes do porta-enxerto Paulsen 1103. Imidacloprid (Provado 200 SC, 40mL 100L-1 e thiamethoxam (Actara 250 WG, 30g 100L-1 reduziram as injúrias causadas pela forma galícola da filoxera nos ponteiros em nível superior a 90%, proporcionando controle superior aos inseticidas deltamethrin (Decis 25 CE, 40mL 100L-1 e fenitrothion (Sumithion 500 CE, 150 mL/100L considerados referência no controle do inseto. Os inseticidas acephate (Orthene 750 BR, 100g 100L-1 e abamectin (Vertimec 18 CE, 80mL 100L-1 não foram eficientes.Grape phylloxera Daktulosphaira vitifoliae (Fitch, 1856 (Hemiptera: Phylloxeridae is the main grape pest. Adults and nymphs feeds on leaves and roots but major damage is observed on roots of own rooted Vitis vinifera. Damage on leaves is important in root stock nurseries where chemical control is necessary. This study was conducted to evaluate insecticides to control the leaf form in a Paulsen 1103 nursery in field condictions. Imidacloprid (Provado 200 SC, 40mL 100L-1 and thiamethoxam (Actara 250 WG, 30g 100L-1 reduced foliar damage in more than 90%, providing better control than deltamethrin (Decis 25 CE, 40mL 100L-1 and fenitrothion (Sumithion 500 CE, 150mL 100L-1 current

  6. Trachyteuthis covacevichi n. sp., a Late Jurassic Palaeopacific coleoid cephalopod

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    D. Fuchs

    2008-02-01

    Full Text Available A new early Oxfordian coleoid cephalopod, Trachyteuthis covacevichi n. sp., is described from northern Chile. It represents the first Late Jurassic Palaeopacific vampyropod and thus considerably extends the palaeogeographic distribution of trachyteuthids. In general, Tr. covacevichi n. sp. possesses a gladius typical for the genus, but wider than in other species. Similarities between Tr. covacevichi n. sp., Tr. palmeri from the Oxfordian of Cuba and Tr. sp. from the Kimmeridgian of Europe confirm a Caribbean Seaway between the Tethys and the Palaeopacific during Late Jurassic times. Morphologically, the wide gladius of Tr. covacevichi n. sp. supports a close phylogenetic relationship between Teudopsis and Trachyteuthis. doi:10.1002/mmng.200700012

  7. OCCURENCE OF PHOMOPSIS SP. ON ACHILLEA MILLEFOLIUM

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    Karolina Vrandečić

    2010-06-01

    Full Text Available During a three year period (2004 to 2006 on locations of Eastern Croatia (Kneževi Vinogradi, Osijek, Brođanci and Krndija we collected plants of Achillea millefolium L. from which we isolated Phomopsis sp. Many of the collected plants exhibited no visible symptoms of infection but in spring pycnidia were noticed on overwintered plants. Isolation was done on PDA from naturally infected plants and morphological and molecular characteristics were studied. Biometrical values of reproductive structures developed on naturally infected plants and grown on PDA were measured. Internal transcribed spacers ITS rDNA of the examined isolates were sequenced. Although molecular analysis showed that isolates were grouped with Diaporthe arctii, we decided to classify isolates from A. millefolium as Phomopsis sp., the details of which were discussed in the paper.

  8. Biodegradation of resorcinol by Pseudomonas sp.

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    Nader Hajizadeh

    2016-11-01

    Full Text Available Objective: To investigate the ability of Pseudomonas sp. isolated from East Azarbaijan, Iran in bioremediation of resorcinol. Methods: Resorcinol biodegradation was evaluated using spectrophotometry and confirmed by gas chromatography-mass spectroscopy. Results: This isolate was able to remove up to 37.12% of resorcinol from contaminated water. Reusability experiments had confirmed the biodegradation process which produced seven intermediate compounds. These intermediates were characterized by gas chromatographymass spectroscopy technique. The products of resorcinol biodegradation were apparently 1, 4-cyclohexadiene, nonadecene, 2-heptadecanone, 1-isopropyl-2-methoxy-4-methylbenzene, hexadecanoic acid, 9-octadecenoic acid, phenol and 5-methyl-2-(1-methylethyl. Conclusions: The findings revealed that Pseudomonas sp. is able to degrade resorcinol. Because of being an indigenous organism, this isolate is more compatible with the climate of the northwest region of Iran and possibly will be used for degradation of other similar aromatic compounds.

  9. Biodegradation of resorcinol byPseudomonas sp.

    Institute of Scientific and Technical Information of China (English)

    Nader Hajizadeh; Najibeh Shirzad; Ali Farzi; Mojtaba Salouti; Azra Momeni

    2016-01-01

    ABSTRACT Objective:To investigate the ability ofPseudomonas sp. isolated from East Azarbaijan, Iran in bioremediation of resorcinol. Methods: Resorcinol biodegradation was evaluated using spectrophotometry and confirmed by gas chromatography-mass spectroscopy. Results:This isolate was able to remove up to 37.12% of resorcinol from contaminated water. Reusability experiments had confirmed the biodegradation process which produced seven intermediate compounds. These intermediates were characterized by gas chromatography-mass spectroscopy technique. The products of resorcinol biodegradation were apparently 1, 4-cyclohexadiene, nonadecene, 2-heptadecanone, 1-isopropyl-2-methoxy-4-methylbenzene, hexadecanoic acid, 9-octadecenoic acid, phenol and 5-methyl-2-(1-methylethyl). Conclusions: The findings revealed thatPseudomonas sp. is able to degrade resorcinol. Because of being an indigenous organism, this isolate is more compatible with the climate of the northwest region of Iran and possibly will be used for degradation of other similar aromatic compounds.

  10. Substitusi Dedak Padi Dengan Pod Kakao(Theobroma cacao L) Dipermentasi Dengan Rhizopus SP, Saccharomyces SP, Lactobacilus SP Terhadap Performans Ternak Babi Perternakan Larance Jantan

    OpenAIRE

    Bornok Venantius P.

    2013-01-01

    BORNOK VENANTIUS P: Substitution of rice bran with cacao pods fermented by Rhizopus sp, Saccharomyces sp and Lactobacillus sp to performance of male croosbred Landrace swine. Under the supervision by NURZAINNAH GINTING and ISKANDAR SEMBIRING. Cacao pods fermented can be used as an alternative feed a substitution of rice bran for its capacity to improve performance during growth. The objective of this research was to prove potention of cacao pods fermented, which can be seen from consumptio...

  11. Two new species of Membranacea Qin & Zhang from China (Hemiptera, Cicadellidae, Typhlocybinae, Empoascini

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    Xiaofei Yu

    2013-01-01

    Full Text Available Two new species of the empoascine leafhopper genus Membranacea Qin & Zhang are reported from China: M. hubeiensis Yu & Yang, sp. n. and M. stenoprocessa Yu & Yang, sp. n.. A key to distinguish all species of the genus is provided.

  12. Notes on the genus Pedionis Hamilton (Hemiptera, Cicadellidae, Macropsinae, and with description of two new species from China

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    Hu Li

    2011-05-01

    Full Text Available Two new species Pedionis (Pedionis nankunshanensis Li, Dai & Li sp. n. and P. (P. tabulatus Li, Dai & Li sp. n. from China are described and illustrated. A key is given to separate all species of this genus (except P. (P. oeroe and P. (P. thyia.

  13. The leafhopper genus Onukigallia Ishihara, 1955 with descriptions of two new species from southern China (Hemiptera, Cicadellidae, Megophthalminae, Agalliini

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    Hu Li

    2016-10-01

    Full Text Available Two new Chinese species of the leafhopper genus Onukigallia, O. neoonukii sp. n. from Sichuan and Guangdong Provinces, and O. tumida sp. n. from Hubei and Hunan Provinces are described and illustrated. A key and an updated checklist with distributions of Onukigallia species are provided.

  14. Two new species of the genus Peltonotellus Puton (Hemiptera: Fulgoromorpha: Caliscelidae) from northwestern China with a world checklist.

    Science.gov (United States)

    Meng, Rui; Gnezdilov, Vladimir M; Wang, Yinglun

    2015-12-04

    A checklist of Peltonotellus species is provided. Two new species, P. brevis sp. nov. from Ningxia Hui Autonomous Region and P. niger sp. nov. from Gansu Province are described and illustrated. Chinese species of the genus Peltonotellus as well as sexual dimorphism are briefly discussed.

  15. Perdas causadas por Coccus viridis (Hemiptera: Coccidae em mudas de Coffea arabica L.

    Directory of Open Access Journals (Sweden)

    Flávio Lemes Fernandes

    2009-08-01

    Full Text Available Coccus viridis (Green danifica plantas jovens e adultas de Coffea arabica Linnaeu. No entanto, nada se sabe sobre a magnitude dos danos causados por esta praga. Assim, este trabalho teve por objetivo estudar as relações entre o ataque de C. viridis e as perdas causadas por este inseto a C. arabica. Este trabalho foi conduzido em casa de vegetação na Universidade Federal de Viçosa. Foram utilizadas sementes da linhagem IAC 15 da variedade “Catuaí vermelho” de café (C. arabica. Para a confecção dos tratamentos esta praga foi criada em casa de vegetação separada do experimento. Os tratamentos foram: plantas infestadas e não infestadas por adultos e ninfas da cochonilha verde. As plantas foram nutridas com solução nutritiva. Durante 110 dias foram avaliados: números de adultos e de ninfas de primeiro, segundo e terceiro ínstares, área foliar, diâmetro do caule, altura das plantas em todas repetições. No final do experimento avaliou-se o peso das raízes, caule, folhas e total. Os pesos das raízes, matéria seca total, área foliar e diâmetro do caule de plantas não atacadas por C. viridis superaram em 1,31; 1,41; 1,50 e 8,93 vezes, respectivamente o peso de plantas atacadas. As variáveis selecionadas foram: diâmetro do caule (cm, área foliar (cm², peso de raízes (g, ninfas, adultos e total das cochonilhas. Concluindo que a planta de C. arabica é afetada de forma diferente entre seus órgãos e que a ninfa de terceiro ínstar e adultos são as fases que mais causam danos a C. arabica.Losses Caused by Coccus viridis (Green (Hemiptera: Coccidae on Seedlings of Coffea arabica L.Abstract. Coccus viridis (Green cause losses on seedling and old plants of Coffea arabica (Green. However, nothing is known about of the damages caused by this pest. Thus, the objective of this work was to evaluate the relations between atack of C. viridis and the losses caused by this insect. on C. arabica. This work was conduced in greenhouse at

  16. Sobre o condicionamento alimentar na cochonilha-branca, Planococcus citri (Risso (Hemiptera: pseudococcidae

    Directory of Open Access Journals (Sweden)

    Lenira Viana Costa Santa-Cecília

    2013-03-01

    Full Text Available Estudos do comportamento alimentar mediante a técnica de "Electrical Penetration Graphs" (EPG das cochonilhas-farinhentas (Pseudococcidae provenientes de um hospedeiro de criação alternativo têm mostrado que esses insetos não atingem ou demoram cerca de 9 horas para alcançar a fase floemática. Por outro lado, aqueles provenientes do hospedeiro-fonte atingem a fase floemática mais rapidamente e apresentam maior frequência de alimentação nos vasos crivados. Esses resultados indicam a presença do fenômeno de condicionamento alimentar, ainda não demonstrado em cochonilhas. Assim, o presente trabalho teve como objetivo determinar a existência desse fenômeno em Planococcus citri (Risso (Hemiptera: Pseudococcidae. Foram realizados testes de livre escolha, monitoramento eletrônico (EPG e estudos de alguns parâmetros biológicos. Em todos os experimentos, o cafeeiro (Coffea arabica L., os citros (Citrus sinensis L. e abóbora (Cucurbita maxima L. foram utilizados como substratos de criação (fonte da cochonilha, sendo os tratamentos constituídos pela combinação entre os hospedeiros-fonte e os hospedeiros receptores (café e citros. O teste de escolha entre cafeeiro e citros nas primeiras 72 horas mostrou que as cochonilhas criadas em cafeeiro apresentaram preferência pelo cafeeiro; aquelas originadas dos citros mostraram uma tendência, embora não significativa, em selecionar os citros em relação ao cafeeiro e aquelas criadas em abóbora não mostraram preferência por nenhum dos hospedeiros. Os estudos do comportamento alimentar mediante o monitoramento eletrônico (EPG mostraram que a fase floemática, considerada como a fase de aceitação do hospedeiro, foi mais frequente em cafeeiro, seja com cochonilhas oriundas deste substrato, seja de citros. Aqueles insetos mantidos em abóbora e transferidos para o cafeeiro ou citros apresentaram excepcionalmente ou não apresentaram nenhuma fase floemática, respectivamente. A

  17. Spøgelsets egne ord

    DEFF Research Database (Denmark)

    Gemzøe, Anker

    2012-01-01

    refleksioner over den gengangerscene, der åbner Shakespeares Hamlet. Ifølge Derridas ’hantologie’ opererer spøgelser i en eksistentiel tidslighed, der har at gøre med sorg, sprog og forvandling. Genremæssigt, tilføjer Gemzøe, har gengangeren det med at husere i tragedien. Med Derridas ’hantologie’ som en af de...

  18. Karyotypic characterization of Capsicum sp. accessions

    OpenAIRE

    Willame Rodrigues do Nascimento Souza; Angela Celis de Almeida; Reginaldo de Carvalho; Regina Lúcia Ferreira; Ana Paula Peron

    2015-01-01

    This study aimed to determine the chromosome number and the karyotype of Capsicum annuum, Capsicum chinense, Capsicum frutencens and Capsicum baccatum accessions in the active Capsicum sp. genebank at the Federal University of Piauí (BGC-UFPI). These species have great economic importance throughout the world, and their cytogenetic characterization can inform taxonomy and lead to improvement in the genus. Karyotypes were obtained from the rootlet meristems of the studied accessions using...

  19. Superfield quantization in Sp(2) covariant formalism

    CERN Document Server

    Lavrov, P M

    2001-01-01

    The rules of the superfield Sp(2) covariant quantization of the arbitrary gauge theories for the case of the introduction of the gauging with the derivative equations for the gauge functional are generalized. The possibilities of realization of the expanded anti-brackets are considered and it is shown, that only one of the realizations is compatible with the transformations of the expanded BRST-symmetry in the form of super translations along the Grassmann superspace coordinates

  20. Det digitale spøgelse

    DEFF Research Database (Denmark)

    Hansen, Ejvind

    2008-01-01

    man opgive at søge ind til én kernebetydning, som kan kommunikeres neutralt gennem forskellige medier. Sætningen “Du er sød” betyder altså noget forskelligt alt efter, om den skrives i et brev, aftegnes på himlen med et fly eller sendes i en e-mail. Og der er ikke én af disse gengivelser, der kan...... påberåbe sig at være den rigtige kernebetydning. Spørgsmålet, der altid kan rejses, er nemlig: Hvad skulle være begrundelsen for at betragte ét medie som værende nærmere kernen end de andre? Man kunne selvfølgelig hævde, at der findes en abstrakt betydning, som ikke kan gengives med noget medie, men som...... til forståelse med den. Omdrejningspunktet er et spøgelse! Artiklens ærinde er at udpege et par af de spøgelser som udspringer af den kommunikation, der udspringer af internettet. Jeg går særligt i klinch med overdrevne forestillinger om internettets effektivitet....

  1. Vorticella sp: Prospective Mosquito Biocontrol agent

    Directory of Open Access Journals (Sweden)

    Chandrashekhar Devidas Patil

    2016-10-01

    Full Text Available Background: Considering the disadvantages of chemical insecticides, we aimed to evaluate Vorticella parasites for control of mosquito larvae of Anopheles stephensi and Aedes aegypti at different larval stages.Methods: Vorticella sp infected mosquito larvae were crushed in the 0.85% saline and homogenized well to get Vorti­cella in suspension. The effects of Vorticella sp infections on larval development were investigated by inoculat­ing protozoan on different larval instars of An. stephensi and Ae. aegypti and observed under light microscope. Le­thal time of the Vorticella infected larvae at different stages was calculated.Results: First and 2nd larval instars of both An. stephensi and Ae. aegypti did not show signs of infection by Vorti­cella sp., whereas 3rd instars of An. stephensi showed more Vorticella infection than those of Ae. aegypti. However, 4th larval instars of both mosquitoes were heavily infected with Vorticella parasite which was responsible for slug­gish movements of larvae and eventually death. Moreover, parasites (Vorticella spp were responsible for more than 90% reduction in adult emergence for both infected An. stephensi and Ae. aegypti.Conclusion: This study provides insights for mosquito larvicidal action of surface parasite Vorticella on different larval stages of An. stephensi and Ae. Aegypti. It could be suggested as a potential candidate in mosquito biocontrol programs.

  2. Sterols from the Madagascar Sponge Fascaplysinopsis sp.

    Directory of Open Access Journals (Sweden)

    Yoel Kashman

    2010-12-01

    Full Text Available The sponge Fascaplysinopsis sp. (order Dictyoceratida, Family Thorectidae from the west coast of Madagascar (Indian Ocean is a particularly rich source of bioactive nitrogenous macrolides. The previous studies on this organism led to the suggestion that the latter should originate from associated microsymbionts. In order to evaluate the influence of microsymbionts on lipid content, 10 samples of Fascaplysinopsis sp. were investigated for their sterol composition. Contrary to the secondary metabolites, the sterol patterns established were qualitatively and quantitatively stable: 14 sterols with different unsaturated nuclei, D5, D7 and D5,7, were identified; the last ones being the main sterols of the investigated sponges. The chemotaxonomic significance of these results for the order Dictyoceratida is also discussed in the context of the literature. The conjugated diene system in D5,7 sterols is known to be unstable and easily photo-oxidized during storage and/or experiments to produce 5a,8a-epidioxy sterols. However, in this study, no 5a,8a-epidioxysterols (or only trace amounts were observed. Thus, it was supposed that photo-oxidation was avoided thanks to the natural antioxidants detected in Fascaplysinopsis sp. by both the DPPH and b-caroten bleaching assays.

  3. Taxonomy Icon Data: Synechocystis sp.PCC 6803 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Synechocystis_sp_PCC_6803_NL.png Synechocystis_sp_PCC_6803_S.png Synechocystis_sp_PCC_6803_NS.png http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=L http://biosciencedbc.jp/taxonomy_ico...n/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=NL http://biosciencedbc.jp/taxonomy_ic...on/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis...+sp%2ePCC+6803&t=NS http://togodb.biosciencedbc.jp/togodb/view/taxonomy_icon_comment_en?species_id=69 ...

  4. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.B...lood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  5. Syrian hamsters (Mesocricetus auratus) with simultaneous intestinal Giardia sp., Spironucleus sp., and trichomonad infections.

    Science.gov (United States)

    Sheppard, Barbara J; Stockdale Walden, Heather D; Kondo, Hirotaka

    2013-11-01

    A commercial facility producing hamsters with a history of infection by dwarf tapeworm (Hymenolepis nana) submitted 15 animals for necropsy and postmortem parasitological and microscopic examination. No tapeworms were detected grossly or microscopically. Fecal examination including gastrointestinal mucosal smears demonstrated mixed intestinal bacteria and low numbers of Giardia sp. Histologic examination of small intestine demonstrated filling of the small intestinal crypts by large numbers of 7-9 µm × 3 µm, rod to crescent or teardrop-shaped flagellates consistent with Spironucleus sp. These organisms had two 1-µm, basophilic, oval nuclei and multiple superficial flagella-like structures. Much larger 10-15 µm × 8-10 µm, oval to pear-shaped organisms were also present in lower numbers and usually located with the crypts. These larger flagellates had multiple flagella and a basophilic rod-shaped nucleus. The larger flagellates included Giardia sp., which had an intimate interface with the surface of the mucosal epithelium, bilaterally symmetry, and binucleation. Lower numbers of trichomonads were also present and were distinguished by an undulating surface membrane and a single nucleus. The mucosa was hyperplastic and moderately inflamed. Although the tapeworm infection was resolved, diagnosis of multiple intestinal flagellates by fecal examination is complicated by the varying sensitivity and diagnostic accuracy of different types of fecal analysis for different flagellate types. Key differences in the morphology and location of the different types of flagellates as observed by histology of intestinal tissues provide important additional diagnostic information to distinguish trichomonads, Spironucleus sp., and Giardia sp.

  6. Aliterella atlantica gen. nov., sp. nov., and Aliterella antarctica sp. nov., novel members of coccoid Cyanobacteria.

    Science.gov (United States)

    Rigonato, Janaina; Gama, Watson Arantes; Alvarenga, Danillo Oliveira; Branco, Luis Henrique Zanini; Brandini, Frederico Pereira; Genuário, Diego Bonaldo; Fiore, Marli Fatima

    2016-09-01

    Two Cyanobacteria isolated from South Atlantic Ocean continental shelf deep water and from a marine green algae inhabiting the Admiralty Bay, King George Island, Antarctica were investigated based on morphological and ultrastructural traits, phylogeny of 16S rRNA gene sequences, secondary structure of the 16S-23S internal transcribed spacer regions and phylogenomic analyses. The majority of these evaluations demonstrated that both strains differ from the genera of cyanobacteria with validly published names and, therefore, supported the description of the novel genus as Aliterella gen. nov. The identity and phylogeny of 16S rRNA gene sequences, together with the secondary structure of D1D1' and BoxB intergenic regions, further supported the two strains representing distinct species: Aliterella atlantica gen. nov., sp. nov. (type SP469036, strain CENA595T) and Aliterella antarctica sp. nov. (type SP469035, strain CENA408T). The phylogenomic analysis of A. atlantica sp. nov. CENA595T, based on 21 protein sequences, revealed that this genus belongs to the cyanobacterial order Chroococcidiopsidales. The isolation and cultivation of two geographically distant unicellular members of a novel cyanobacterial genus and the sequenced genome of the type strain bring new insights into the current classification of the coccoid group, and into the reconstruction of their evolutionary history.

  7. Ten new species of Rhagovelia in the angustipes complex (Hemiptera: Heteroptera: Veliidae) from Colombia, with a key to the Colombian species.

    Science.gov (United States)

    Padilla-Gil, Dora Nancy

    2015-12-21

    Ten new species of Rhagovelia are described from Colombia as follows: Rhagovelia penta sp. n., and Rhagovelia santanderi sp. n., from the Upper and Middle Magdalena River Valley respectively; Rhagovelia carina sp. n., Rhagovelia tricoma sp. n., and Rhagovelia barbacoensis sp. n., from Altaquer, Río Ñambi; Rhagovelia caunapi sp. n. from Río Caunapi; Rhagovelia tumaquensis sp. n., from Tumaco, Río Mejicano; Rhagovelia jagua sp. n., from Eastern Andes; Rhagovelia mocoa sp. n. and Rhagovelia umbria sp. n., from Amazonas region. Rhagovelia tantilla Drake & Harris is recorded from Colombia, for the first time; the description of macropterous morph of Rhagovelia espriella Padilla-Gil, 2011 and a key to the Rhagovelia, angustipes complex of Colombian species are provided.

  8. The genus Hypheodana Metcalf and description of three new species (Hemiptera, Cicadomorpha, Membracidae O gênero Hypheodana Metcalf e descrição de três novas espécies (Hemiptera, Cicadomorpha, Membracidae

    Directory of Open Access Journals (Sweden)

    Albino M. Sakakibara

    2005-12-01

    Full Text Available The genus Hypheodana Metcalf, 1952, previously considered of uncertain position, is classified in Darninae-Darnini. The following species are treated, together with a re-characterization of the genus: Hypheodana ursus (Fairmaire, 1846, H. cuneata (Fowler, 1894 comb. nov. (formerly in Aspona, H. intermedia (Fowler, 1894 comb. nov. (formerly in Aspona, and also H. proxima sp. nov. (from Costa Rica, H. costata sp. nov. and H. acuta sp. nov. (these two from Mato Grosso, Brazil. All the species are illustrated.O gênero Hypheodana Metcalf, 1952, antes considerado em posição incerta, é classificado em Darninae-Darnini. As seguintes espécies são tratadas, juntamente com uma re-caracterização do gênero: Hypheodana ursus (Fairmaire, 1846, H. cuneata (Fowler, 1894 comb. nov. (antes em Aspona, H. intermedia (Fowler, 1894 comb. nov. (antes em Aspona e, também, H. proxima sp. nov. (da Costa Rica, H. costata sp. nov. e H. costata sp. nov. e H. acuta sp. nov. (estas duas do Mato Grosso, Brasil. Todas as espécies são ilustradas.

  9. EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill

    Directory of Open Access Journals (Sweden)

    SARMILA TASNIM

    2013-05-01

    Full Text Available Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%.

  10. Descriptions of a New Genus and Species of Leafhopper (Hemiptera: Cicadellidae: Mukariinae) Attacking Chimonobambusa (Gramineae: Bambusoideae) from Guizhou Province, China%危害方竹的叶蝉一新属新种记述(半翅目:叶蝉科:额垠叶蝉亚科)

    Institute of Scientific and Technical Information of China (English)

    李子忠; 陈祥盛; 张斌

    2007-01-01

    In this paper, 1 new genus and 1 new species of leafhopper subfamily Mukariinae (Hemiptera: Cicadellidae) from Guizhou Province, China, Pseudomohunia gen. nov. and P. nigrifascia sp. nov., attacking Chimonobambusa Makino (Gramineae: Bambusoideae), are described and illustrated. This new genus is closely related to Mohunia Distant, but can be distinguished from the latter by the forewing with 4 apical cells, the aedeagus with 2 gonopores, dorsoatrium short, and the connective Y-shaped. All type specimens are deposited in the Institute of Entomology, Guizhou University.%记述采自我国贵州省雷公山国家级自然保护区狭叶方竹上的叶蝉科昆虫1新属1新种,即拟痕叶蝉属和黑带额痕叶蝉.根据头部颜面构造、前后翅端部翅脉、腿节刚毛公式、下生殖板及阳基侧突等特征,该属应隶属于额垠叶蝉亚科,与痕叶蝉属近缘,主要区别特征为:前翅具4个端室(后者前翅具3个端室);阳茎具短背腔(后者阳茎简单,无背腔),有2个性孔(后者仅1个性孔);连索"V"形(后者连索"Y"形).模式标本保存于贵州大学昆虫研究所.

  11. First record of Clonostachys rosea (Ascomycota: Hypocreales) as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa (Hemiptera: Cicadellidae) in Argentina.

    Science.gov (United States)

    Toledo, A V; Virla, E; Humber, R A; Paradell, S L; Lastra, C C López

    2006-05-01

    Clonostachys rosea (Link: Fries) Schroers, Samuels, Seifert, and Gams (Ascomycota: Hypocreales) has been reported as a mycoparasite of fungi and nematodes and as saprobe in soils, but this fungus has not been reported previously to be entomopathogenic. Many species of cicadellid leafhoppers cause economic damage to crops as vectors of plant pathogens. In the present work, we report the first record of C. rosea as an entomopathogenic fungus of two leafhoppers pest, Oncometopia tucumana and Sonesimia grossa (Hemiptera: Cicadellidae), in Argentina and evaluate the pathogenicity of C. rosea against them.

  12. Description of the immature stages and new host plant records of Deois (Deois) mourei (Berg) (Hemiptera: Cercopidae), a species newly recorded from Argentina and Paraguay.

    Science.gov (United States)

    Foieri, Alvaro; Lenicov, Ana M Marino De Remes; Virla, Eduardo G

    2016-09-06

    Deois (Deois) mourei Cavichioli & Sakakibara (Hemiptera: Cercopidae) is recorded for the first time from Argentina and Paraguay. The eggs and immature stages of the species are described and illustrated; the main characters that distinguish instars are body size, color, number of flagellomeres, and number of tibial and metatarsomere spines. A key for identification of nymphs of D. (D.) mourei and a key to differentiate nymphs of the sympatric species D. (D.) mourei and Notozulia entreriana Berg are provided. In addition, a list of host plants of D. (D.) mourei in Argentina is given.

  13. Use of Dactylopius coccus Costa (Hemiptera: Coccoidea) pigments in the detection of larvae and pupae of Plutella xylostella (L.) (Lepidoptera: Yponomeutidae).

    Science.gov (United States)

    Goche, D; Vigueras, A L; Portillo, L; Llanderal, C

    2012-06-01

    Carmines obtained from the dye of Dactylopius coccus Costa (Hemiptera: Coccoidea) were used for the detection of larvae and pupae of Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) in broccoli inflorescences. Larvae were dyed with carmine II and red cochineal, while the majority of the dyes, with the exception of carmine III and the aqueous extract, were suitable to dye pupae. In the broccoli lumps exposed to the dyes, only the verge of the stems were actually dyed, right in the position where the incision took place, an appropriate characteristic for implementing this technique for commercial use.

  14. Asociaciones áfido-parasitoide (Hemiptera: Aphididae; Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos en Los Cardales, Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    Andrea V. ANDORNO

    2007-01-01

    Full Text Available Diez especies de áfidos (Hemiptera: Aphididae se hallaron parasitados por siete especies de parasitoides (Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos. Myzus persicae (Sulzer fue el áfido más frecuentemente encontrado sobre una amplia variedad de cultivos, y con mayor diversidad de parasitoides asociados. Aphidius colemani Viereck fue el afidiino más usual, que ataca varias especies de áfidos. Ocho asociaciones tritróficas, involucrando Aphidius matricariae Haliday, han sido registradas por primera vez para la Argentina.

  15. Descriptions of the immature stages and new host plant records of Notozulia entreriana (Berg) (Hemiptera: Cercopidae) pests of grasses in subtropical areas of the Americas.

    Science.gov (United States)

    Foieri, Alvaro; Lenicov, Ana M Marino De Remes; Virla, Eduardo G

    2016-04-11

    Notozulia entreriana (Berg) (Hemiptera: Cercopidae) is one of the most common spittlebugs inhabiting the subtropical region of the America, inflicting important economic damage to grass crops. The immature stages are described and illustrated; the main characteristics that distinguish instars are the body size, color, number of flagellomeres, and number of tibial and metatarsomere spines. A key for identification of nymphs is provided as a tool to develop field studies.  Nine host plants, all belonging to Poaceae, are recorded as breeding and feeding host plants from different localities in northern Argentina.

  16. Primeiro registro da ocorrência de mosca-negra-dos-citros, Aleurocanthus woglumi Ashby, 1915 (Hemiptera: Sternorrhyncha: Aleyrodidae) em Roraima.

    OpenAIRE

    Correia, Ruy Guilherme; Lima, Antonio Cesar Silva; Farias,Paulo Roberto Silva; Maciel, Francisco Clemilton da Silva; Silva, Marcos Wanderley da; da Silva, Anderson Gonçalves

    2012-01-01

    A mosca-negra-dos-citros, Aleurocanthus woglumi Ashb, (Hemiptera: Aleyrodidae) é uma importante praga dos citros. De origem asiática, esta praga foi detectada no Brasil pela primeira vez no estado do Pará no ano de 2001. Na cultura dos citros a mosca-negra acarreta danos diretos e indiretos, prejudicando o desenvolvimento e produção das plantas. Além de restringir o comércio de locais de sua ocorrência para áreas livres da presença da praga, visto que o A. woglumi é considerado praga quarente...

  17. Macrosiphoniella remaudierei, a new species of aphid on Helichrysum in Iran (Hemiptera, Aphididae)

    Science.gov (United States)

    Barbagallo, Sebastiano; Nieto Nafría, Juan M.

    2016-01-01

    Abstract A new species of aphid, belonging to the genus Macrosiphoniella Del Guercio, 1911, is described using three samples collected in Iran on Helichrysum armenium (Asteraceae, Inuleae) by the late Prof. G. Remaudière. Both apterous and alate viviparous females of the new taxon, Macrosiphoniella remaudierei sp. n., are described and compared to corresponding morphs of the closely allied Macrosiphoniella aetnensis and to other congeneric aphid species on Helichrysum in the Palaearctic region. Type specimens are now stored in the Muséum national d’Histoire naturelle in Paris. PMID:28138287

  18. A new species of Sulcana (Hemiptera: Cicadellidae: Gyponini from the Brazilian Atlantic Forest

    Directory of Open Access Journals (Sweden)

    Alexandre Cruz Domahovski

    2015-10-01

    Full Text Available ABSTRACT Sulcana cunicula sp. nov. is described and illustrated based on four specimens from municipality of São José dos Pinhais, state of Paraná, Brazil. This species can be distinguished from others in the genus by the following characters: (1 head produced, slightly shorter than median length of pronotum; (2 aedeagus bearing a pair of acute ventral processes at mid-length of shaft and extended dorsally. A new record to Sulcana brevis DeLong & Freytag and a key to the three species of the genus are also presented.

  19. A new genus of the tribe Caliscelini (Hemiptera, Fulgoroidea, Caliscelidae) from Vietnam.

    Science.gov (United States)

    Gnezdilov, Vladimir M; Bourgoin, Thierry; Soulier-Perkins, Adeline

    2014-12-22

    A new genus Annamatissus Gnezdilov et Bourgoin gen. nov., including the new species, Annamatissus tami Gnezdilov et Soulier-Perkins sp. nov. is described in the family Caliscelidae from the Bi-Doup massif in Lam Dong Province of Vietnam. The new taxon represents only the second genus of the tribe Caliscelini known from Vietnam. An identification key to separate Gelastissus Kirkaldy from Annamatissus gen. nov. is provided together with a check list of the Caliscelidae of Vietnam and their distribution. New distribution data in Vietnam are given for Cicimora sicildia Emeljanov, 1998 and Gelastissus hokutonis (Matsumura, 1916).

  20. Macrosiphoniella remaudierei, a new species of aphid on Helichrysum in Iran (Hemiptera, Aphididae

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    Sebastiano Barbagallo

    2017-01-01

    Full Text Available A new species of aphid, belonging to the genus Macrosiphoniella Del Guercio, 1911, is described using three samples collected in Iran on Helichrysum armenium (Asteraceae, Inuleae by the late Prof. G. Remaudière. Both apterous and alate viviparous females of the new taxon, M. remaudierei sp. n., are described and compared to corresponding morphs of the closely allied M. aetnensis and to other congeneric aphid species on Helichrysum in the Palaearctic region. Type specimens are now stored in the Muséum national d’Histoire naturelle in Paris.

  1. Antibacterial Activity of Freshwater Microalga Scenedesmus sp. on Foodborne Pathogens Staphylococcus aureus and Salmonella sp.

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    Aisha Gogoba Ishaq

    2016-01-01

    Full Text Available Microalgae possess promising bioactive compounds with a wide range of activities. In this study, crude pigment extract of Scenedesmus sp. a freshwater microalga was screened against two known foodborne pathogens Staphylococcus aureus (ATCC 25923 and Salmonella sp. (ATCC 14028 to evaluate its antimicrobial properties. The crude pigment of the microalga was extracted with 90 % acetone whereas antibacterial screening was done by agar well diffusion method. In addition, the MIC of crude pigment was determined following the macrobroth dilution method. Concentrations ranging from 0.35mg/ml – 3.48 mg/ml demonstrated highest inhibitory activity against Staphylococcus aureus (ATCC 25923.  The MIC was achieved at 0.08 mg/ ml. On the other hand, there was no inhibitory activity at any concentration of crude extract against Salmonella sp. (ATCC 14028.  Results of this study suggest that the inhibitory activity may be limited to gram positive foodborne pathogen Staphylococcus aureus.

  2. Direct transesterification of Oedogonium sp. oil be using immobilized isolated novel Bacillus sp. lipase.

    Science.gov (United States)

    Sivaramakrishnan, Ramachandran; Muthukumar, Karuppan

    2014-01-01

    This work emphasizes the potential of the isolated Bacillus sp. lipase for the production of fatty acid methyl ester by the direct transesterification of Oedogonium sp. of macroalgae. Dimethyl carbonate was used as the extraction solvent and also as the reactant. The effect of solvent/algae ratio, water addition, catalyst, temperature, stirring and time on the direct transesterification was studied. The highest fatty acid methyl ester yield obtained under optimum conditions (5 g Oedogonium sp. powder, 7.5 ml of solvent (dimethyl carbonate)/g of algae, 8% catalyst (%wt/wt of oil), distilled water 1% (wt/wt of algae), 36 h, 55°C and 180 rpm) was 82%. Final product was subjected to thermogravimetric analysis and (1)H NMR analysis. The results showed that the isolated enzyme has good potential in catalyzing the direct transesterification of algae, and the dimethyl carbonate did not affect the activity of the isolated lipase.

  3. Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae en el estado brasileño de "Rio Grande do Sul" Triatoma delpontei Romaña & Abalos, 1947 (Hemiptera, Triatominae in Rio Grande do Sul State, Brasil

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    Roberto Salvatella Agrelo

    1993-02-01

    Full Text Available Triatoma delpontei Romaña y Abalos, 1947 es una especie de triatomineo silvestre, de marcada ornitofilia, que se asocia preferentemente al psitácido Myiopsitta monachus (Boddaert, 1783 colonizando sus nidos. Especie que se identifica con zonas de forestas subtropicales secas o xerofíticas propias de las provincias biogeográficas continentales chaqueñas, es notificada para Brasil en la microregión de la campaña de Río Grande do Sul (barra de Quaraí, Municipio de Uruguaiana.Triatoma delpontei (Romaña & Abalos, 1947 (Hemiptera, Triatominae is an ornithophilic sylvatic with a particular association to the psittacid Myiopsitta monachus (Boaddert, 1783. It is found in the continental biogeographical province of the Chaco, where it inhabits the nests or M.monachus, in subtropical xerophytic forests. The authors report the first finding of T. delpontei in Brasil, in the "campanha" region of the State or Rio Grande do Sul (Barra do Quarai, Uru-guaiana, on the right bank of the River Cuareim, not far from de Uruguayan border.

  4. Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.

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    R.F. Silva

    2013-01-01

    Full Text Available Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L-1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L-1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L-1 in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites.

  5. Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas).

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    Endo, Akihito; Futagawa-Endo, Yuka; Schumann, Peter; Pukall, Rüdiger; Dicks, Leon M T

    2012-03-01

    Five strains of bifidobacteria were isolated from faeces of a common marmoset (Callithrix jacchus) and a red-handed tamarin (Saguinus midas). The five isolates clustered inside the phylogenetic group of the genus Bifidobacterium but did not show high sequence similarities between the isolates and to known species in the genus by phylogenetic analysis based on 16S rRNA gene sequences. Sequence analyses of dnaJ1 and hsp60 also indicated their independent phylogenetic positions to each other in the Bifidobacterium cluster. DNA G+C contents of the species ranged from 57.3 to 66.3 mol%, which is within the values recorded for Bifidobacterium species. All isolates showed fructose-6-phosphate phosphoketolase activity. Based on the data provided, the five isolates represent five novel species, for which the names Bifidobacterium reuteri sp. nov. (type strain: AFB22-1(T) = JCM 17295(T) = DSM 23975(T)), Bifidobacterium callitrichos sp. nov. (type strain: AFB22-5(T) = JCM 17296(T) = DSM 23973(T)), Bifidobacterium saguini sp. nov. (type strain: AFB23-1(T) = JCM 17297(T) = DSM 23967(T)), Bifidobacterium stellenboschense sp. nov. (type strain: AFB23-3(T) = JCM 17298(T) = DSM 23968(T)) and Bifidobacterium biavatii sp. nov. (type strain: AFB23-4(T) = JCM 17299(T) = DSM 23969(T)) are proposed.

  6. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

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    Katsuhiro Ueda

    2009-12-01

    Full Text Available A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549.

  7. Two-Dimensional Phosphorus Carbide: Competition between sp(2) and sp(3) Bonding.

    Science.gov (United States)

    Guan, Jie; Liu, Dan; Zhu, Zhen; Tománek, David

    2016-05-11

    We propose previously unknown allotropes of phosphorus carbide (PC) in the stable shape of an atomically thin layer. Different stable geometries, which result from the competition between sp(2) bonding found in graphitic C and sp(3) bonding found in black P, may be mapped onto 2D tiling patterns that simplify categorizing of the structures. Depending on the category, we identify 2D-PC structures that can be metallic, semimetallic with an anisotropic Dirac cone, or direct-gap semiconductors with their gap tunable by in-layer strain.

  8. Two new species of the sharpshooter genus Oragua Melichar, 1926 from Southeastern Brazil (Hemiptera: Cicadellidae: Cicadellini).

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    Mejdalani, Gabriel; Silva, Cristiane M F Da; Takiya, Daniela M

    2016-01-01

    Two very similar new species of Oragua Melichar, 1926, O. linnavuorii sp. nov. and O. flavolineata sp. nov., are described and illustrated from the Atlantic Forest of Southeastern Brazil (Mantiqueira mountain range). The new species share the presence of a large dentiform process located posteroventrally on the male pygofer, a unique feature within the genus. They can be distinguished from each other by the paraphyses with a pair of elongate, slender asymmetrical rami in O. linnavuorii and with two pairs of short, spiniform apical processes in O. flavolineata. The male of O. linnavuorii is macropterous but the female is submacropterous and thus smaller than the former when specimens are measured from the apex of the crown to the tips of the forewings at rest. The female of O. flavolineata is not known. Male holotypes are deposited in DZRJ. A key to males of the five Oragua species recorded from Southeastern Brazil is provided [O. maculifera Young, 1977, O. discoidula (Osborn, 1926), O. triplehorni Young, 1977, O. linnavuorii, and O. flavolineata]. The superficially similar genera Oragua and Aguana Melichar, 1926 are briefly compared.

  9. A review of the legged mealybugs on bamboo (Hemiptera: Coccoidea: Pseudococcidae) occurring in China.

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    Li, Wen-Chao; Tsai, Ming-Yu; Wu, San-An

    2014-12-23

    Twenty-two species of legged mealybugs occur on bamboo (Poaceae: Bambusoideae) in China. These species are reviewed and two of them are described for the first time: Dysmicoccus luoyangensis Li & Wu, sp. nov. and Trionymus indocalamus Li & Wu, sp. nov. Two new combinations are established: Pseudococcus bambusicola Takahashi and Kaicoccus bambusus Wu are transferred to genus Formicococcus Takahashi as F. bambusicola (Takahashi) comb. nov. and F. bambusus (Wu) comb. nov., respectively. Formicococcus bambusicola, Heliococcus bambusae (Takahashi), Neoripersia miscanthicola Takahashi and Trionymus formosanus Takahashi are redescribed and reillustrated based on type specimens. Brief notes are provided for Balanococcus kwoni Pellizari & Danzig, B. zhejiangensis Li & Wu, Dysmicoccus dengwuensis Ferris, D. indocalamus Wu, Eumyrmococcus smithii Silvestri, Ferrisicoccus angustus Ezzat & McConnell, Heliococcus lingnaniae Wang, H. takae (Kuwana), Heterococcus abludens Borchsenius, Miscanthicoccus miscanthi (Takahashi), Palmicultor lumpurensis (Takahashi), Paraporisaccus guizhouensis Lu & Wu, Paraserrolecanium fargesii Wu, Pseudantonina magnotubulata Borchsenius and Trionymus bambusae (Green). A key to the species of legged mealybugs on bamboo occurring in China is given. A list of bamboo mealybugs worldwide is also provided. 

  10. The complete mitochondrial genome sequence of Geisha distinctissima(Hemiptera: Flatidae) and comparison with other hemipteran insects

    Institute of Scientific and Technical Information of China (English)

    Nan Song; Aiping Liang

    2009-01-01

    The complete nucleotide sequence of the mitochondrial genome (mitogenome) of Geisha distinctissima (Hemiptera: Flatidae) has been determined in this study. The genome is a circular molecule of 15,971 bp with a total A + T content of 75.1%. The gene content, order, and structure are consistent with the Drosophila yakuba genome structure and the hypothesized ancestral arthropod genome arrangement. All 13 protein-coding genes are observed to have a putative, inframe ATR methion-ine or ATT isoleucine codons as start signals. Canonical TAA and TAG termination codons are found in nine protein-coding genes, and the remaining four (coxl, atp6, cox3, and nad4) have incomplete termination codons. The anticodons of all transfer RNA (tRNAs) are identical to those observed in D. yakuba and Philaenus spumarius, and can be folded in the form of a typical clover-leaf structure except for tRNASer(AGN). The major non-coding region (the A + T-rich region or putative control region) between the small ribosomal subunit and the tRNAIle gene includes two sets of repeat regions. The first repeat region consists of a direct 152-bp repetitive unit located near the srRNA gene end, and the second repeat region is composed of a direct repeat unit of 19 bp located toward tRNAIle gene. Comparisons of gene variability across the order suggest that the gene content and arrangement of G. distinctissima mitogenome are similar to other hemipteran insects.

  11. Developmental biology, polymorphism and ecological aspects of Stiretrus decemguttatus (Hemiptera, Pentatomidae, an important predator of cassidine beetles

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    Lucia Maria Paleari

    2013-03-01

    Full Text Available Developmental biology, polymorphism and ecological aspects of Stiretrus decemguttatus (Hemiptera, Pentatomidae, an important predator of cassidine beetles. Stiretrus decemguttatus is an important predator of two species of cassidine beetles, Botanochara sedecimpustulata (Fabricius, 1781 and Zatrephina lineata (Fabricius, 1787 (Coleoptera, Cassidinae, on the Marajó Island, Brazil. It attacks individuals in all development stages, but preys preferentially on late-instar larvae. Its life cycle in the laboratory was 43.70 ± 1.09 days, with an egg incubation period of six days and duration from nymph and adult stages of 16.31 ± 0.11 and 22.10 ± 1.67 days, respectively. The duration of one generation (T was 12.65 days and the intrinsic population growth rate (r 0.25. These data reveal the adjustment of the life cycle of S. decemgutattus with those of the two preys, but suggest greater impact on Z. lineata. However, no preference over cassidine species was shown in the laboratory. Up to 17 different color patterns can be found in adults of S. decemguttatus, based on combinations of three basic sets of color markings. Some of them resemble the markings of chrysomelids associated with Ipomoea asarifolia (Convolvulaceae and are possibly a mimetic ring. Three color patterns were identified in nymphs, none of which was associated with any specific adult color pattern.

  12. Putting scales into evolutionary time: the divergence of major scale insect lineages (Hemiptera) predates the radiation of modern angiosperm hosts.

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    Vea, Isabelle M; Grimaldi, David A

    2016-03-22

    The radiation of flowering plants in the mid-Cretaceous transformed landscapes and is widely believed to have fuelled the radiations of major groups of phytophagous insects. An excellent group to test this assertion is the scale insects (Coccomorpha: Hemiptera), with some 8,000 described Recent species and probably the most diverse fossil record of any phytophagous insect group preserved in amber. We used here a total-evidence approach (by tip-dating) employing 174 morphological characters of 73 Recent and 43 fossil taxa (48 families) and DNA sequences of three gene regions, to obtain divergence time estimates and compare the chronology of the most diverse lineage of scale insects, the neococcoid families, with the timing of the main angiosperm radiation. An estimated origin of the Coccomorpha occurred at the beginning of the Triassic, about 245 Ma [228-273], and of the neococcoids 60 million years later [210-165 Ma]. A total-evidence approach allows the integration of extinct scale insects into a phylogenetic framework, resulting in slightly younger median estimates than analyses using Recent taxa, calibrated with fossil ages only. From these estimates, we hypothesise that most major lineages of coccoids shifted from gymnosperms onto angiosperms when the latter became diverse and abundant in the mid- to Late Cretaceous.

  13. ASPECTOS BIOLÓGICOS DE Euborellia annulipes (DERMAPTERA: ANISOLABIDIDAE ALIMENTADA COM O PULGÃO Hyadaphis foeniculi (HEMIPTERA: APHIDIDAE

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    ALDENI BARBOSA DA SILVA

    2010-01-01

    Full Text Available The cultivation of sweet herb of great economic importance for small farmers in the micro and Agrest Swamp of Paraíba and also in the state of Pernambuco. Among the natural enemies, the scissors Euborellia annulipes proves to be a voracious predator, that is, with high ability to attack and feed on different prey, particularly of eggs and immature stages of insects of Lepidoptera, Hemiptera, Coleoptera and Diptera. The objective of this research was to study the biological aspects of E. annulipes on H. foeniculi laboratory. The study was conducted at the Entomology Laboratory, Department of Plant Protection at the UFPB in a climatic chamber, at 25 ± 1 ºC, relative humidity of 70 ± 10% RH and 12 hours. Scissors were selected in adulthood from rearing on artificial diet in the laboratory. From these insects have made observations of posture, viability and morphology of eggs of E. annulipes. Fifty nymphs were selected for each stage of development (1, 2, 3, 4 and 5 of the scissors and fed separately with aphids (1st-2nd, 3rd and 4th instar. There has been evaluation regarding the number and duration of instars. There are five instars to the nymphal stage of E. annulipes, the average incubation period was 12.9 days, pre-oviposition period of 18.2 days and 46.75 eggs per clutch, there was an increase of nymphal E. annulipes in that it offered up more aphids developed.

  14. Molecular phylogenetics of cixiid planthoppers (Hemiptera: Fulgoromorpha): new insights from combined analyses of mitochondrial and nuclear genes.

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    Ceotto, Paula; Kergoat, Gaël J; Rasplus, Jean-Yves; Bourgoin, Thierry

    2008-08-01

    The planthopper family Cixiidae (Hemiptera: Fulgoromorpha) comprises approximately 160 genera and 2000 species divided in three subfamilies: Borystheninae, Bothriocerinae and Cixiinae, the later with 16 tribes. The current paper represents the first attempt to estimate phylogenetic relationships within Cixiidae based on molecular data. We use a total of 3652bp sequence alignment of four genes: the mitochondrial coding genes Cytochrome c Oxidase subunit 1 (Cox1) and Cytochrome b (Cytb), a portion of the nuclear 18S rDNA and two non-contiguous portions of the nuclear 28S rDNA. The phylogenetic relationships of 72 terminal specimens were reconstructed using both maximum parsimony and Bayesian inference methods. Through the analysis of this empirical dataset, we also provide comparisons among different a priori partitioning strategies and the use of mixture models in a Bayesian framework. Our comparisons suggest that mixture models overcome the benefits obtained by partitioning the data according to codon position and gene identity, as they provide better accuracy in phylogenetic reconstructions. The recovered maximum parsimony and Bayesian inference phylogenies suggest that the family Cixiidae is paraphyletic in respect with Delphacidae. The paraphyly of the subfamily Cixiinae is also recovered by both approaches. In contrast to a morphological phylogeny recently proposed for cixiids, subfamilies Borystheninae and Bothriocerinae form a monophyletic group.

  15. Influence of Type of Electric Bright Light on the Attraction of the African Giant Water Bug, Lethocerus indicus (Hemiptera: Belostomatidae

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    Luke Chinaru Nwosu

    2012-01-01

    Full Text Available This study investigated the influence of type of electric bright light (produced by fluorescent light tube and incandescent light bulb on the attraction of the African giant water bug, Lethocerus indicus (Hemiptera: Belostomatidae. Four fluorescent light tubes of 15 watts each, producing white-coloured light and four incandescent light bulbs of 60 watts each, producing yellow-coloured light, but both producing the same amount of light, were varied and used for the experiments. Collections of bugs at experimental house were done at night between the hours of 8.30 pm and 12 mid-night on daily basis for a period of four months per experiment in the years 2008 and 2009. Lethocerus indicus whose presence in any environment has certain implications was the predominant belostomatid bug in the area. Use of incandescent light bulbs in 2009 significantly attracted more Lethocerus indicus 103 (74.6% than use of fluorescent light tubes 35 (25.41% in 2008 [4.92=0.0001]. However, bug’s attraction to light source was not found sex dependent [>0.05; (>0.18=0.4286 and >0.28=0.3897]. Therefore, this study recommends the use of fluorescent light by households, campgrounds, and other recreational centres that are potentially exposed to the nuisance of the giant water bugs. Otherwise, incandescent light bulbs should be used when it is desired to attract the presence of these aquatic bugs either for food or scientific studies.

  16. Sublethal Effects of Cyantraniliprole and Imidacloprid on Feeding Behavior and Life Table Parameters of Myzus persicae (Hemiptera: Aphididae).

    Science.gov (United States)

    Zeng, Xianyi; He, Yingqin; Wu, Jiaxing; Tang, Yuanman; Gu, Jitao; Ding, Wei; Zhang, Yongqiang

    2016-08-01

    The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an agricultural pest that seriously infests many crops worldwide. This study used electrical penetration graphs (EPGs) and life table parameters to estimate the sublethal effects of cyantraniliprole and imidacloprid on the feeding behavior and hormesis of M. persicae The sublethal concentrations (LC30) of cyantraniliprole and imidacloprid against adult M. persicae were 4.933 and 0.541 mg L(-1), respectively. The feeding data obtained from EPG analysis indicated that the count probes and number of short probes (imidacloprid-treated plants. In addition, the phloem-feeding behavior of M persicae was significantly impaired on fed tobacco plants treated with cyantraniliprole and imidacloprid at LC30 Analysis of life table parameters indicated that the growth and reproduction of F1 generation aphids were significantly affected when initial adults were exposed to LC30 of cyantraniliprole and imidacloprid. The nymphal period, female longevity, total preoviposition period, and mean generation time were significantly prolonged when initial adults were exposed to LC30 of imidacloprid. By comparison, these parameters were prolonged but not significantly in the cyantraniliprole treatment. The fecundity and gross reproductive rate were significantly increased in the treated groups. Similarly, the net reproductive rate was greater in the treated group than the control group. Our results indicate that treatment with LC30 of imidacloprid and cyantraniliprole would lead to a hormetic response of M. persicae, with higher likelihood of occurrence when initial adults were exposed to LC30 of cyantraniliprole.

  17. The secondary contact zone of phylogenetic lineages of the Philaenus spumarius (Hemiptera: Aphrophoridae): an example of incomplete allopatric speciation.

    Science.gov (United States)

    Lis, Agata; Maryańska-Nadachowska, Anna; Lachowska-Cierlik, Dorota; Kajtoch, Lukasz

    2014-01-01

    Previous studies on the phylogeography of the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) suggest the existence of a contact zone of its main phylogenetic lineages along mountain chains in Europe and western Asia. This study presents a detailed examination of the population genetics of P. spumarius within the Carpathian Mountains. The main objective was to determine whether the populations inhabiting that area consist of individuals belonging to different genetic units and whether the observed pattern could be an example of secondary contact zone which formed after incomplete allopatric speciation. Specimens from six transects across the Carpathian arc were examined. The mitochondrial phylogeography of the meadow spittlebug in the examined area clearly shows that individuals from both main clades meet and mix there. Representatives of all three main EF1-α clades were also found. The present distribution of the main clades with a zone of overlap along the mountain ranges may suggest that these phylogenetic lineages form a young hybrid zone. Moreover, a limited number of individuals were shown to possess heteroplasmic mitochondrial DNA, which gives additional support to intraspecific hybridization. P. spumarius could be used in future work as an excellent model species in investigating population genetics, intraspecific hybridization, and speciation in progress.

  18. The secondary contact zone of phylogenetic lineages of the Philaenus spumarius (Hemiptera: Aphrophoridae: Cercopidae): an example of incomplete allopatric speciation.

    Science.gov (United States)

    Lis, Agata; Maryańska-Nadachowska, Anna; Lachowska-Cierlik, Dorota; Kajtoch, Łukasz

    2014-01-01

    Previous studies on the phylogeography of the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae: Cercopidae) suggest the existence of a contact zone of its main phylogenetic lineages along mountain chains in Europe and western Asia. This study presents a detailed examination of the population genetics of P. spumarius within the Carpathian Mountains. The main objective was to determine whether the populations inhabiting that area consist of individuals belonging to different genetic units and whether the observed pattern could be an example of secondary contact zone which formed after incomplete allopatric speciation. Specimens from six transects across the Carpathian arc were examined. The mitochondrial phylogeography of the meadow spittlebug in the examined area clearly shows that individuals from both main clades meet and mix there. Representatives of all three main EF1-α clades were also found. The present distribution of the main clades with a zone of overlap along the mountain ranges may suggest that these phylogenetic lineages form a young hybrid zone. Moreover, a limited number of individuals were shown to possess heteroplasmic mitochondrial DNA, which gives additional support to intraspecific hybridization. P. spumarius could be used in future work as an excellent model species in investigating population genetics, intraspecific hybridization, and speciation in progress.

  19. Performance of Three Adelphocoris spp. (Hemiptera:Miridae) on Flowering and Non-lfowering Cotton and Alfalfa

    Institute of Scientific and Technical Information of China (English)

    GAO Zhen; PAN Hong-sheng; LIU Bing; LU Yan-hui; LIANG Ge-mei

    2014-01-01

    The genus Adelphocoris (Hemiptera:Miridae) is a group of key insect pests in cotton ifelds in China that includes three dominant species:A. suturalis (Jakovlev), A. lineolatus (Goeze) and A. fasciaticollis (Reuter). Previous ifeld surveys have found that adults of these Adelphocoris species usually move onto speciifc host plants when the plants enter the lfowering stage. To determine the potential trade-offs for this host-plant preference behavior, the performance of these three Adelphocoris spp. on lfowering and non-lfowering cotton and alfalfa were compared in the laboratory. The results showed that Adelphocoris spp. had signiifcantly higher nymphal developmental and survival rates, along with increased adult longevity and fecundity on lfowering cotton and alfalfa than on non-lfowering plants of either species. In addition, compared with cotton plants, alfalfa generally promoted better performance of these three Adelphocoris species, especially for A. lineolatus. Simple correlation analysis indicated that female adult longevity was positively correlated to male adult longevity and female fecundity, and female fecundity was positively correlated to nymphal development and survival rates. This study demonstrated a positive correlation between adult preference and offspring/adult performance for all three Adelphocoris species, with no evidence of any trade-offs for this preference for lfowering host plants. These ifndings support the hypothesis that hemimetabolous insects such as mirid bugs have positive adult preference-adult/nymphal performance relationships, which is likely due to the similar feeding habits and nutritional requirements of adults and nymphs.

  20. Structure and Sensilla of the Mouthparts of the Spotted Lanternfly Lycorma delicatula (Hemiptera: Fulgoromorpha: Fulgoridae, a Polyphagous Invasive Planthopper.

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    Yanan Hao

    Full Text Available Mouthparts are among the most important sensory and feeding structures in insects and comparative morphological study may help explain differences in feeding behavior and diet breadth among species. The spotted lanternfly Lycorma delicatula (White (Hemiptera: Fulgoromorpha: Fulgoridae is a polyphagous agricultural pest originating in China, recently established and becoming widespread in Korea, and more recently introduced into eastern North America. It causes severe economic damage by sucking phloem sap and the sugary excrement produced by nymphs and adults serves as a medium for sooty mold. To facilitate future study of feeding mechanisms in this insect, the fine-structural morphology of mouthparts focusing on the distribution of sensilla located on the labium in adult L. delicatula was observed using a scanning electron microscope. The mouthparts consist of a small cone-shaped labrum, a tubular labium and a stylet fascicle consisting of two inner interlocked maxillary stylets partially surrounded by two shorter mandibular stylets similar to those found in other hemipteran insects. The five-segmented labium is unusual (most other Fulgoromorpha have four segments and is provided with several types of sensilla and cuticular processes situated on the apex of its distal labial segment. In general, nine types of sensilla were found on the mouthparts. Six types of sensilla and four types of cuticular processes are present on sensory fields of the labial apex. The proposed taxonomic and functional significance of the sensilla are discussed. Morphological similarities in the interlocking mechanism of the stylets suggest a relationship between Fulgoromorpha and Heteroptera.