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Sample records for cantharidin

  1. Cantharidin toxicosis in 2 alpacas

    OpenAIRE

    Simpson, Katharine M.; Streeter, Robert N.; de Souza, Patricia; Genova, Suzanne G.; Morgan, Sandra E.

    2013-01-01

    Two adult alpacas were presented for recumbency and reluctance to rise. Cantharidin toxicosis was suspected based on clinical and ancillary diagnostic findings. The diagnosis was confirmed by gas chromatography-mass spectrometry of gastric contents and urine. Despite medical treatment, neither alpaca survived. Blister beetle toxicosis has not been previously described in camelids. Challenges in treatment of affected ruminants or pseudoruminants are noted.

  2. Inhibitory Effect of Cantharidin on Proliferation of A549 Cells

    Institute of Scientific and Technical Information of China (English)

    WANG Xiao-hua; YIN Yuan-qin; SUI Cheng-guang; MENG Fan-dong; MA Ping; JIANG You-hong

    2007-01-01

    Objective: To study the inhibition of Cantharidin against the proliferation of human lung cancer A549 cells and its mechanism. Methods: MTT assay was employed to determine the inhibition of Cantharidin against proliferation of A549 cells and flow Cytometry was applied to analyze A549 cell cycle and the effect of Cantharidin on cell cycle. Results: Cantharidin showed inhibition against the proliferation of A549 cells, and the inhibition was mediated by blocking A549 cell cycle at G2/M phase significantly. Conclusion: Cantharidin exhibits inhibition against the proliferation of human lung cancer A549 cells.

  3. A Novel Approach to the Quantitation of Coeluting Cantharidin and Deuterium Labelled Cantharidin in Blister Beetles (Coleop-tera: Meloidae

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    W Boland

    2007-11-01

    Full Text Available Blister beetles (Coleoptera: Meloidae are the main natural source of cantharidin, but the compound titre is depended on several factors including, age, sex and mating status of the insects. In order to eliminate such uncertainty factors in physio¬logical and chemical studies deuterium labelled cantharidin (D2C with no natural abundance is normally introduced into the beetles' body to use it as a model for studying the cantharidin behaviour in vivo. Experiments were achieved on Mylabris quadripunctata (Col.: Meloidae from Southern France and the beetles were exposed to an artificial diet containing a de¬fined amount of D2C. On the other hand, because of the high similarity between the two compounds they cannot be well quantified by gas chromatography. In order to remove the burden, MRM technique was used for the first time which could successfully create well-defined cantharidin and D2C peaks and hence a precise measurement. MRM technique was exam¬ined using a GC-MS Varian Saturn which collected MS/MS data of more than one compound in the same time window of the chromatogram. It is especially useful when coeluting compounds have different parent ions, i.e. m/z 84 for D2C (coelut¬ing isotopically-labelled compound and m/z 82 for cantharidin (beetle-originated compound. Using the routine GC-MS runs, measurement accuracy may be significantly reduced because the D2C peak is covered by the cantharidin huge peak while MRM could reveal the two coincided peaks of cantharidin and D2C. Therefore MRM is hereby introduced as the method of choice to separate cantharidin from D2C with high sensitivity and thus provide a precise base of quantitation.

  4. A Novel Approach to the Quantitation of Coeluting Cantharidin and Deuterium Labelled Cantharidin in Blister Beetles (Coleop-tera: Meloidae

    Directory of Open Access Journals (Sweden)

    MR Nikbakhtzadeh

    2007-12-01

    Full Text Available Blister beetles (Coleoptera: Meloidae are the main natural source of cantharidin, but the compound titre is depended on several factors including, age, sex and mating status of the insects. In order to eliminate such uncertainty factors in physio¬logical and chemical studies deuterium labelled cantharidin (D2C with no natural abundance is normally introduced into the beetles' body to use it as a model for studying the cantharidin behaviour in vivo. Experiments were achieved on Mylabris quadripunctata (Col.: Meloidae from Southern France and the beetles were exposed to an artificial diet containing a de¬fined amount of D2C. On the other hand, because of the high similarity between the two compounds they cannot be well quantified by gas chromatography. In order to remove the burden, MRM technique was used for the first time which could successfully create well-defined cantharidin and D2C peaks and hence a precise measurement. MRM technique was exam¬ined using a GC-MS Varian Saturn which collected MS/MS data of more than one compound in the same time window of the chromatogram. It is especially useful when coeluting compounds have different parent ions, i.e. m/z 84 for D2C (coelut¬ing isotopically-labelled compound and m/z 82 for cantharidin (beetle-originated compound. Using the routine GC-MS runs, measurement accuracy may be significantly reduced because the D2C peak is covered by the cantharidin huge peak while MRM could reveal the two coincided peaks of cantharidin and D2C. Therefore MRM is hereby introduced as the method of choice to separate cantharidin from D2C with high sensitivity and thus provide a precise base of quantitation.

  5. Study on effects of cantharidin on cutaneous leishmaniasis, its mechanism and optimization of the therapeutic modes

    Directory of Open Access Journals (Sweden)

    Fatemeh Maleki

    2015-11-01

    Full Text Available Leishmaniasis is one of the major problems in many countries. Leishmania is flagellated protozoa and causative agent of leishmaniasis which is the most important health problem in many countries especially in developing country. Leishmania major causes cutaneous leishmaniasis (CL. CL is endemic in some part of Iran. Pentavalent antimony compounds are main therapy of CL, they have some side effects due to their toxicity, and also relapse is possible. Cantharidin is terpenoid and vesicant compound that can be found in Meloidae and Oedomeridae family beetles. It was used as treatment to cancer and Wart. It is also apoptosis inducer in various cancer cells. In this study, the effect of 0.5, 1, 2, 5, 10, 20 and 50 µg/ml cantharidin on the L. major promastigotes, non-infected macrophages and infected macrophages with parasite amastigotes was studied by (3-(4,5-dimethyl thiazolyl-2-2,5-diphenyle tetrazolium bromide MTT assay and flow cytometer in vitro. The Effect of cantharidin as 0.5, 0.05 and 0.1% ointment surveyed on the Leishmania lesions in BALB/C as well as. Parasite load as determined by Real Time PCR, and IFN-γ and IL-4 was involved by ELISA. Results showed that the highest cytotoxicity (56.14% in promastigotes was in a group that treated with 50 µg/ml cantharidin after 48h. The rate in non-infected macrophages and infected macrophages was 13.05 % and 30.17% respectively. Maximum cytotoxicity rate in promastigotes treated with 50 µg/ml cantharidin after 72 h was determined 66.48%, 48.52% in non- infected macrophages and 62.24% in infected macrophages after 48h by flow cytometry. Group treated with 0.05% cantharidin had lowest rate of ulcer growth. Ulcer size was increased in group treated with 0.5% cantharidin. IFN-γ value in group treated with cantharidin was less than it in untreated (control group, but IL -4 didn’t change. Cantharidin through blister formation induces inflammatory reaction and neutrophils and macrophages infiltration

  6. Norcantharidin, Derivative of Cantharidin, for Cancer Stem Cells

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    Chen-Hsi Hsieh

    2013-01-01

    Full Text Available Cancer stem cells (CSCs existing in human cancers have been demonstrated to be a major cause of cancer treatment resistance, invasion, metastasis, and relapse. Self-renewal pathways, Wnt/β-catenin, Sonic hedgehog (Shh, and the Notch signaling pathway play critical roles in developing CSCs and lead to angiogenesis, migration, invasion, and metastasis. Multidrug resistance (MDR is an unfavorable factor causing the failure of treatments against cancer cells. The most important and thoroughly studied mechanism involved in MDR is the active efflux of chemotherapeutic agents through membrane drug transporters. There is growing evidence that Norcantharidin (NCTD, a water-soluble synthetic small molecule derivative of naturally occurring cantharidin from the medicinal insect blister beetle (Mylabris phalerata Pallas, is capable of chemoprevention and tumor inhibition. We summarize investigations into the modulation of self-renewal pathways and MDR in CSCs by NCTD. This review may aid in further investigation of using NCTD to develop more effective strategies for cancer treatment to reduce resistance and recurrence.

  7. Treatment of mid-late stage NSCLC using sodium cantharidinate/vitamin B6/GP regimen in clinic

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    Baoli Wang

    2014-01-01

    Conclusion: Sodium cantharidinate/vitamin B6/GP regimen had fair effectiveness and synergistically improved the clinical outcomes. It lowered the toxic/adverse effects and its application is worth further investigation and promotion.

  8. Characterization of glutathione S-transferases from Sus scrofa, Cydia pomonella and Triticum aestivum: their responses to cantharidin.

    Science.gov (United States)

    Yang, Xue-Qing; Zhang, Ya-Lin

    2015-02-01

    Glutathione S-transferases (GSTs) play a key role in detoxification of xenobiotics in organisms. However, their other functions, especially response to the natural toxin cantharidin produced by beetles in the Meloidae and Oedemeridae families, are less known. We obtained GST cDNAs from three sources: Cydia pomonella (CpGSTd1), Sus scrofa (SsGSTα1), and Triticum aestivum (TaGSTf3). The predicted molecular mass is 24.19, 25.28 and 24.49 kDa, respectively. These proteins contain typical N-terminal and C-terminal domains. Recombinant GSTs were heterologously expressed in Escherichia coli as soluble fusion proteins. Their optimal activities are exhibited at pH 7.0-7.5 at 30 °C. Activity of CpGSTd1 is strongly inhibited by cantharidin and cantharidic acid, but is only slightly suppressed by the demethylated analog of cantharidin and cantharidic acid. Enzymatic assays revealed that cantharidin has no effect on SsGSTα1 activity, while it significantly stimulates TaGSTf3 activity, with an EC50 value of 0.3852 mM. Activities of these proteins are potently inhibited by the known GST competitive inhibitor: S-hexylglutathione (GTX). Our results suggest that these GSTs from different sources share similar structural and biochemical characteristics. Our results also suggest that CpGSTd1 might act as a binding protein with cantharidin and its analogs.

  9. Physicochemical Properties and Gastric Mucosa Irritation of Cantharidin-hydroxypropyl-β-cyclodextrin Inclusion Complex

    Institute of Scientific and Technical Information of China (English)

    AN Lin-na; DANG Yun-jie; HU Chun-hui; ZHU Chun-yan

    2012-01-01

    Objective To increase the solubility and relieve the mucous irritation of cantharidin (CA) by preparing cantharidin-hydroxypropyl-β-cyclodextrin (CA/HP-β-CD) inclusion complex.Methods The inclusion complex was prepared by co-evaporation method and characterized by differential scanning calorimetry (DSC),X-ray diffractometry (XRD),and nuclear magnetic resonance (NMR).Results The disappearance of CA as well as the shift of exothermic peaks shown in DSC results indicated the complexation phenomenon.XRD results showed that the crystalline CA pattern had disappeared,and in NMR results,H-5 shifted from δ 3.731to 3.695 after complexation and H-2 shifted from δ 3.626 to 3.598,which suggested that part of the drug had entered the HP-β-CD cavity to form an inclusion complex.The solubility increased 10.3 times after complexation and the mucous irritation of CA was relieved remarkably.Conclusion Through complexation with HP-β-CD,the solubility and dissolution rate of CA are improved significantly,and the irritation of musous is relieved.

  10. Identification and Biochemical Characterization of Protein Phosphatase 5 from the Cantharidin-Producing Blister Beetle, Epicauta chinensis

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    Xi'en Chen

    2013-12-01

    Full Text Available Protein phosphatase 5 (PP5 is a unique member of serine/threonine phosphatases which has been recognized in regulation of diverse cellular processes. A cDNA fragment encoding PP5 (EcPP5 was cloned and characterized from the cantharidin-producing blister beetle, E. chinensis. EcPP5 contains an open reading frame of 1500 bp that encodes a protein of 56.89 kDa. The deduced amino acid sequence shares 88% and 68% identities to the PP5 of Tribolium castaneum and humans, respectively. Analysis of the primary sequence shows that EcPP5 has three TPR (tetratricopeptide repeat motifs at its N-terminal region and contains a highly conserved C-terminal catalytic domain. RT-PCR reveals that EcPP5 is expressed in all developmental stages and in different tissues. The recombinant EcPP5 (rEcPP5 was produced in Escherichia coli and purified to homogeneity. The purified protein exhibited phosphatase activity towards pNPP (p-nitrophenyl phosphate and phosphopeptides, and its activity can be enhanced by arachidonic acid. In vitro inhibition study revealed that protein phosphatase inhibitors, okadaic acid, cantharidin, norcantharidin and endothall, inhibited its activity. Further, protein phosphatase activity of total soluble protein extract from E. chinensis adults could be impeded by these inhibitors suggesting there might be some mechanism to protect this beetle from being damaged by its self-produced cantharidin.

  11. Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling

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    Yi-Ping Huang

    2013-01-01

    Full Text Available Cancer metastasis becomes an initial cause of cancer death in human population. In many cancers, it has been shown that the high levels of matrix metalloproteinase (MMP-2 and/or MMP-9 are associated with the invasive phenotypes of cancer cells. In this study, we investigated the effects of cantharidin, a derivative of blister beetles which is one of the traditional Chinese medicines, on the adhesion, migration, and invasion of human bladder cancer TSGH-8301 cells. Cantharidin effectively suppressed TSGH-8301 cell adhesion, migration, and invasion in a concentration-dependent manner. Results from Western blotting, RT-PCR, and gelatin zymography assays indicated that cantharidin blocked the protein levels, gene expression (mRNA, and activities of MMP-2 and -9 in TSGH-8301 cells. Cantharidin also significantly suppressed the protein expressions of p-p38 and p-JNK1/2 in TSGH-8301 cells. Taken together, cantharidin was suggested to present antimetastatic potential via suppressing the levels of MMP-2 and MMP-9 expression that might be mediated by targeting the p38 and JNK1/2 MAPKs pathway in TSGH-8301 human bladder cancer cells.

  12. Investigation of two blood proteins binding to Cantharidin and Norcantharidin by multispectroscopic and chemometrics methods

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Rong; Cheng, Zhengjun, E-mail: ncczj1112@126.com; Li, Tian; Jiang, Xiaohui

    2015-01-15

    The interactions of Cantharidin/Norcantharidin (CTD/NCTD) with two blood proteins, i.e., bovine serum albumin (BSA) and bovine hemoglobin (BHb), have been investigated by the fluorescence, UV–vis absorption, and FT-IR spectra under imitated physiological condition. The binding characteristics between CTD/NCTD and BSA/BHb were determined by fluorescence emission and resonance light scattering (RLS) spectra. The quenching mechanism of two blood proteins with CTD/NCTD is a static quenching. Moreover, the experimental data were further analyzed based on multivariate curve resolution-alternating least squares (MCR-ALS) technique to obtain the concentration profiles and pure spectra for three species (BSA/BHb, CTD/NCTD and CTD/NCTD–BSA/BHb complexes) which existed in the interaction procedure. The number of binding sites n and binding constants K{sub b} were calculated at various temperatures. The thermodynamic parameters (such as, ΔG, ΔH, and ΔS) for BSA–CTD/NCTD and BHb–CTD/NCTD systems were calculated by the Van’t Hoff equation and also discussed. The distance r between CTD/NCTD and BSA/BHb were evaluated according to Förster no-radiation energy transfer theory. The results of Fourier transform infrared (FT-IR), synchronous fluorescence and three-dimensional fluorescence spectra showed that the conformations of BSA/BHb altered with the addition of CTD/NCTD. In addition, the effects of common ions on the binding constants of BSA–CTD/NCTD and BHb–CTD/NCTD systems were also discussed.

  13. Laryngeal edema induced by disodium cantharidinate injection%斑蝥酸钠注射液致喉头水肿

    Institute of Scientific and Technical Information of China (English)

    陈小蕾; 吴立强; 赵青

    2013-01-01

      1例72岁男性患者,确诊为左肺腺癌7个月,既往有小牛血清去蛋白过敏史。治疗期间输入斑蝥酸钠注射液约30 min,患者突然烦躁,急发憋闷、喉痒、发声嘶哑、吸气性呼吸困难,口唇明显发绀,三凹征阳性等。立即停止输入,给予氧气吸入,甲强龙、异丙嗪、葡萄糖酸钙抗过敏以及其他对症治疗,约20 min后,患者症状明显改善,口唇发绀及三凹征消失。%  One 72-year-old male patient was diagnosed with left lung adenocarcinoma 7 months ago. He had a history of deproteinised calf blood serum injection allergy. About 30 minutes after the disodium cantharidinate injection was used, the patient suddenly developed dysphoria, oppressed, throat itching, voice hoarse, inspiratory dyspnea, obvious lips hematocyanosis, three depression sign positive and so on. This medicine was immediately stopped. The patient received oxygen inhalation and the therapy of antianaphylaxis by methylprednisolone, promethazine hydrochloride injection, calcium gluconate and other treatments. After about 20 minutes, the symptoms improved obviously, lips hematocyanosis and three depression sign disappeared.

  14. A novel cantharidin analog N-Benzylcantharidinamide reduces the expression of MMP-9 and invasive potentials of Hep3B via inhibiting cytosolic translocation of HuR

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ji-Yeon; Chung, Tae-Wook; Choi, Hee-Jung [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Lee, Chang Hyun [Department of Anatomy, College of Korean Medicine, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Eun, Jae Soon; Han, Young Taek [College of Pharmacy, Woosuk University, Wanju-gun, Jeonbuk (Korea, Republic of); Choi, Jun-Yong [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Kim, So-Yeon; Han, Chang-Woo [Department of Internal Medicine, Korean Medicine Hospital, School of Korean Medicine, Pusan National University, Yangsan 626-870 (Korea, Republic of); Jeong, Han-Sol, E-mail: jhsol33@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Ha, Ki-Tae, E-mail: hagis@pusan.ac.kr [Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of); Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam (Korea, Republic of)

    2014-05-02

    Highlights: • We examined the inhibition of N-Benzylcantharidinamide on MMP-9-mediated invasion. • Unlike cantharidin, N-Benzylcantharidinamide has very low toxicity on Hep3B cells. • The reduced MMP-9 expression was due to HuR-mediated decrease of mRNA stability. • We suggest N-Benzylcantharidinamide as a novel inhibitor of MMP-9-related invasion. - Abstract: Invasion and metastasis are major causes of malignant tumor-associated mortality. The present study aimed to investigate the molecular events underlying inhibitory effect of N-Benzylcantharidinamide, a novel synthetic analog of cantharidin, on matrix metalloproteinase-9 (MMP-9)-mediated invasion in highly metastatic hepatocellular carcinoma Hep3B cells. In this investigation, among six analogs of cantharidin, only N-Benzylcantharidinamide has the inhibitory action on MMP-9 expression at non-toxic dose. The MMP-9 expression and invasion of Hep3B cells were significantly suppressed by treatment of N-Benzylcantharidinamide in a dose-dependent manner. On the other hand, the transcriptional activity of MMP-9 promoter and nuclear levels of NF-κB and AP-1 as the main transcriptional factors inducing MMP-9 expression were not affected by it although the level of MMP-9 mRNA was reduced by treatment of N-Benzylcantharidinamide. Interestingly, the stability of MMP-9 mRNA was significantly reduced by N-Benzylcantharidinamide-treatment. In addition, the cytosolic translocation of human antigen R (HuR), which results in the increase of MMP-9 mRNA stability through interaction of HuR with 3′-untranslated region of MMP-9 mRNA, was suppressed by treatment of N-Benzylcantharidinamide, in a dose-dependent manner. Taken together, it was demonstrated, for the first time, that N-Benzylcantharidinamide suppresses MMP-9 expression by reducing HuR-mediated MMP-9 mRNA stability for the inhibition of invasive potential in highly metastatic Hep3B cells.

  15. 斑蝥素对小菜蛾体壁组织结构的影响%Effect of cantharidin on the integument structure of Plutella xylostella

    Institute of Scientific and Technical Information of China (English)

    陈利平; 杨宝东; 张志勇; 张爱环; 张民照; 张来喜; 孙淑玲

    2011-01-01

    To investigate the contact toxicity of the natural insect product cantharidin ( C10 H12 04, CTD) against a Lepidopteran insect, the diamondback moth Plutella xylostella ( L. ) , transmission electron microscope (TEM ) images of P. Xylostella larvae were observed at different times after treatment with CTD. Exposure to a sublethal dose of 0. 15 μg/ larva and a medial lethal dose of 0. 3 μg/larva for 12 h and 24 h by topical application to the pronotum gave similar results; larvaes' bodies became dark and they died 12 h after treatment. TEM images of the epidermal cells of treated larvae showed that cell nuclei had aggregated, mitochondria had become distorted with mitochondrial cristae and the mitochondria had become blurred and vacuolizated. The quantity of mitochondria and rough endoplasmic reticulum ( RER) declined 12 h after CTD treatment. These phenomena were also evident 24 h after CTD treatment. These results indicate that CTD did not destroy the cuticle layers but damaged epidermal cells in the insects' integument.%为探究昆虫天然产物斑蝥素( cantharifin,CTD)对鳞翅目昆虫小菜娥Plutella xylostella(L.)的触杀机制,利用透射电子显微镜观察了该物质处理小菜蛾4龄幼虫体壁组织结构的变化.斑蝥素亚致死剂量0.15 μg/头和半致死剂量0.3μg/头点滴处理试虫前胸背板,中毒试虫反映结果类似.处理12 h后中毒试虫虫体发黑,陆续死亡.处理12 h后,体壁细胞细胞核固缩并且边极化,线粒体扭曲,内脊模糊出现空泡化,线粒体和粗面内质网数量下降;处理24 h后,体壁细胞出现的类似处理12 h后的症状,且症状更为明显.结果表明CTD对小菜蛾体壁表皮层组织结构无显著破坏作用,但对体壁细胞有明显的致毒作用.

  16. Study on bacterial endotoxins test of disodium cantharidinate and vitamin B_6 injection%斑蝥酸钠维生素B_6注射液细菌内毒素检查法研究

    Institute of Scientific and Technical Information of China (English)

    王贵英; 唐继坤; 潘正兴

    2009-01-01

    Objective:To establish a method for determination of the bacterial endotoxin test in disodium cantharidinate and vitamin B_6 injection.Method:Inhibition and bacterial endotoxin test was performed with amebcyte lysate manufactured by different companies with different specifications according to the method of bacterial endotoxin test approved by Chinese Pharmacopoeia 2005 edition.Results:The original solution had non-interference with the bacterial endotoxin test.The results of bacteria endotoxin test in six batchs all accorded with the standard of the quality control.Conclusion:The results suggested that bacteria endotoxin test can be used as an alternative method for the rabbit pyrogen test for disodium cantharidinate and vitamin B_6 injection.The limits of bacterial endotoxin was 6 EU·mg~(-1).%目的:建立斑蝥酸钠维生素B6注射液的细菌内毒素检查方法.方法:按中国药典2005年版二部附录XIE细菌内毒素检查法进行,用小同厂家不同规格的鲎试剂对小同批号的斑蝥酸钠维牛素B_6注射液进行了干扰试验和细菌内毒素检查.结果:本品原液对细菌内毒素检查无干扰作用.按拟定标准检验,该品种6批样品细菌内毒素检查结果均符合规定.结论:本品可用细菌内毒素检查法代替家兔热原检查法,其细菌内毒素的限值(L)为6 EU·mL~(-1).

  17. 高效液相色谱法测定不同单一食物下短翅豆芫菁体内斑蝥素的含量%Quantitative determination of cantharidin in Epicauta aptera Kaszab with different food by HPLC

    Institute of Scientific and Technical Information of China (English)

    吕春颖; 周建威

    2013-01-01

    运用高效液相色谱法测定不同单一食物下短翅豆芫菁体内斑蝥素的含量.采用HPLC法,色谱柱为C18柱,流动相为甲醇:水(28:72),检测波长为230nm,进样量为20μL,柱温为30℃.用丙酮氯仿在酸性条件下回流提取虫粉.处理的6个样品中,在西红柿叶喂养条件下短翅豆芫菁体内斑蝥素的含量高于马兰叶.%Quantitative determination of cantharidin in Epicauta aptera Kaszab with different food by HPLC. Waters 2487 HPLC and C18 column was used to set the mobile phase at methanol-water (28: 72) , the column temperature is 30℃, the flow rate is lmL/min, the detection wavelength is 230nm, With acetone and chloroform in acid conditions backflow is to extract insect powder. The cantharidin content in the tomato leaves feeding conditions is higher than Kalimeris indica leaves in dealing with six samples.

  18. Study on the HPLC Fingerprints of Spanish fly Ultra-fine Powder from Different Producing Areas and Content Determination of Cantharidin%不同产地斑蝥超微粉体的HPLC指纹图谱及斑蝥素的含量测定

    Institute of Scientific and Technical Information of China (English)

    何杰; 张水寒; 李跃辉; 万丹; 黄江波

    2014-01-01

    目的:结合斑蝥主要成分斑蝥素的含量测定与指纹图谱,比较不同产地药材化学成分的差异性,为科学评价不同产地斑蝥药材及其超微粉体的质量提供依据.方法:建立斑蝥超微粉体指纹图谱检测方法.采用高效液相色谱(HPLC)法对各产地斑蝥超微粉体进行分析,采用中药色谱指纹图谱相似度评价系统软件比较指纹图谱的相似度,采用SPSS 13.0统计软件对10批样品进行聚类分析,并测定斑蝥素含量.结果:不同产地斑蝥超微粉体指纹图谱有较大相似性,但斑蝥素的含量有一定的差异.聚类分析结果表明,不同产地斑蝥药材存在一定差异,但差别不大.结论:所建立的HPLC指纹图谱具有较好的精密度、重复性和稳定性,结合斑蝥素的含量,可为斑蝥药材及其超微粉体质量评价提供依据.%OBJECTIVE:To compare the difference of chemical components in Spanish fly from different producing areas on the basis of content determination and fingerprints of cantharidin,and to provide reference for quality control of S.fly from different producing areas and its ultra-fine powder.METHODS:Established the fingerprint method of S.fly ultra-fine powder.S.fly ultra-fine powder were determined by HPLC.Chromatographic Fingerprint Similarity Evaluation software was used to analyze the similarity of HPLC fingerprint.Cluster analysis was conducted in 10 batches of sample by using SPSS 13.0 software,and the content of cantharidin was determined.RESULTS:The fingerprints of S.fly ultra-fine powder from different producing areas were similar to each other.The content of cantharidin had little difference.Cluster analysis showed that there was little difference in S.fly from different producing areas.CONCLUSIONS:Established HPLC fingerprint is precise,reproducible and stable,and combined with content determination of cantharidin to provide reference for quality evaluation of S.fly and its ultra-fine powder.

  19. Disodium Cantharidinate and Vitamin B6 Injection plus Chemotherapy for Non-Small Cell Lung Cancer: A Systematic Review%斑蝥酸钠维生素B6注射液联合化疗治疗非小细胞肺癌的系统评价

    Institute of Scientific and Technical Information of China (English)

    盛蕾; 李岩; 陈健鹏

    2012-01-01

    目的 系统评价斑蝥酸钠维生素B6注射液联合化疗治疗非小细胞肺癌( Non-small cell lung carcinoma,NSCLC)的有效性及安全性.方法 计算机检索Cochrane图书馆临床对照试验资料库、MEDLINE、EMbase、CBM、CNKI、VIP等数据库,检索时限为从1966年至2011年11月,纳入斑蝥酸钠维生素B6注射液联合化疗治疗NSCLC的随机对照试验.由2名评价者独立评价并交叉核对纳入研究质量后,对同质研究采用RevMan 5.1软件进行Meta分析.结果 经筛选,最终纳入8个RCT,共539例受试者.文献质量评价结果显示,8篇均为B级.Meta分析结果表明:与对照组相比,斑蝥酸钠维生素B6注射液能提高NSCLC化疗的有效率[RR=1.32,95%CI( 1.07,1.62)]、临床受益率[RR=1.24,95%CI (1.12,1.37)],改善患者生活质量[RR=2.23,95%CI( 1.55,3.19)]、临床症状[RR=1.55,95%CI( 1.24,1.95)],增加患者体重[RR=2.72,95%CI (1.74,4.25)],并减轻骨髓抑制毒性作用(白细胞下降率)[RR=0.36,95%CI (0.21,0.61)].结论 斑蝥酸钠维生素B6注射液对NSCLC患者接受化疗能起到增效减毒作用.为获得更佳的循证医学证据,建议开展更多大样本、多中心、长期随访的高质量临床随机对照试验.%Objective To systematically evaluate the effectiveness and safety of disodium cantharidinate and vita-rain B6 injection plus chemotherapy compared with chemotherapy alone, in the treatment of non-small cell lung cancer (NSCLC). Methods The Cochrane Library (Issue 1, 2011), MEDLINE (1966 to November 2011), EMbase (1984 to November 2011), CBM (1978 to November 2011), CNKI (1995 to November 2011) and VIP (1989 to November 2011) were searched electronically, and the randomized controlled trials (RCTs) about disodium cantharidinate and vitamin B6 injection plus chemotherapy for NSCLC were included. The quality of the included studies was assessed and crosschecked by two reviewers independently, and meta-analyses were performed for homogeneous

  20. 斑蝥酸钠维生素B6注射液联合奈达铂治疗胸腔积液的疗效分析%Analysis of the efficacy of cantharidin sodium vitamin B6 injection combined with nedaplatin treatment of pleural effusion

    Institute of Scientific and Technical Information of China (English)

    王吉天; 付桂莲

    2013-01-01

    目的 观察斑蝥酸钠维生素B6注射液联合奈达铂胸腔注入治疗恶性胸腔积液的疗效和不良反应.方法 将73例肺癌并发恶性胸腔积液患者随机分为观察组(38例)和对照组(35例),观察组患者用一次性中心静脉引流专用导管胸腔置管闭式引流胸腔积液后,胸腔内注射斑蝥酸钠维生素B6注射液和奈达铂;对照组患者胸腔内仅注射奈达铂.观察组胸腔给药:斑蝥酸钠维生素B6注射液50 ml+生理盐水20ml+奈达铂100 mg;对照组胸腔给药:奈达铂100 mg+生理盐水70 ml.结果 观察组患者的临床总有效率和生活质量改善率均优于对照组(均P<0.05),两组患者不良反应差异无统计学意义(P>0.05).结论 胸腔灌注斑蝥酸钠维生素B6注射液联合奈达铂治疗恶性胸腔积液的疗效优于单用奈达铂,不良反应轻.%Objective To observe the efficacy and adverse reactions of cantharidin sodium vitamin B6 injection combined with nedaplatin pleural injection for the treatment of malignant pleural effusion.Methods 73 cases of lung cancer complicated by patients with malignant pleural effusions,central venous drainage catheter chest tube closed drainage of pleural effusion,given the intrathoracic drug injection,cantharidin sodium vitamin B6 injection combined with of nedaplatin(observation group 38 cases),with nedaplatin only(control group,35 cases).observation Group pleural administration:the cantharides sodium vitamin B6 injection 50ml + saline 20ml + nedaplatin 100mg; control group pleural administration:nedaplatin 100mg + saline 70ml.Results Observation group of patients with clinical total rate of efficiency and improved quality of life was better than in control group (P < 0.05),similar to the two sets of adverse reactions.Conclusions Pleural perfusion the sodium cantharidate vitamin B6 injection in conjunction with nedaplatin treatment of malignant pleural effusion effective than single nedaplatin,and the side effects

  1. Research on the role of natural cantharidin to trichomonas vaginalis%天然产物斑蝥素对阴道毛滴虫作用的研究

    Institute of Scientific and Technical Information of China (English)

    李晓飞

    2013-01-01

    目的 体外观察斑蝥素对8 株抗甲硝唑阴道毛滴虫的杀灭效果及安全性.方法 以斑蝥素终质量浓度为15.313~490.000 μg/mL 作用于体外培养的阴道毛滴虫.结果 30.625 μg/mL 为斑蝥素体外杀灭阴道毛滴虫的最低有效质量浓度.结论 斑蝥素对体外阴道毛滴虫有杀灭效果.

  2. Molluscum contagiosum

    Science.gov (United States)

    ... lesions may result in scarring. Medicines, such as salicylic acid preparations used to remove warts , may be helpful. But these medicines can cause blistering that leads to temporary skin discoloration. Cantharidin, commonly called beetle juice, is the ...

  3. Determination of cantharidin,nitrogen mustard and minoxidil in nurturing hair products by liquid chromatography-tandem mass spectrometry%育发产品中斑蝥素和氮芥及米诺地尔的液相色谱-联质谱测定法

    Institute of Scientific and Technical Information of China (English)

    郑磊; 胡小键; 林少彬

    2016-01-01

    目的 建立了育发产品中斑蝥素、氮芥和米诺地尔的液相色谱-串联质谱(LC-MS-MS)测定方法.方法 样品经直接稀释1000倍,以95%甲醇(含0.2%氨水)-5%乙腈为流动相进行等度洗脱,选用Waters symmetry C18(4.6 mm×250 mm,5 μm)色谱柱,正离子模式下质谱多反应监测(MRM)模式检测.结果 斑蝥素、氮芥和米诺地尔分别在5.00~500、5.00~1000和0.100~50.0 ng/ml的范围内,所得的回归方程均呈较好的线性关系(r≥0.999),检出限分别为2.0、3.4、0.04μg/g定量下限分别为6.4、11.0、0.10μg/g,回收率在94.5%~104.7%之间,RSD<9%.结论 该方法灵敏度高,选择性强,适用于育发产品中斑蝥素、氮芥和米诺地尔的测定.

  4. Cantharidin patches and intravenous administration of vitamin C in the concomitant treatment of herpes zoster:a case report%斑蝥素贴剂结合维生素C静脉注射辅助治疗带状疱疹1例

    Institute of Scientific and Technical Information of China (English)

    Martin Schencking; Karin Kraft

    2011-01-01

    @@ The diagnosis and therapy for herpes zoster (HZ) are frequently associated with consultation of a general practitioner and with hospital care.Although there are several serious complications of zoster such as ophthalmic, splanchnic, cerebral,and motor conditions, the most common and feared in immunocompetent adults is postherpetic neuralgia (PHN).Its definition is controversial.Recent data support the distinction between acute herpetic neuralgia (within 30 d of rash onset),subacute herpetic neuralgia (30 to 120 d after rash onset), and postherpetic neuralgia (defined as pain lasting at least 120 d from rash onset)[1-3].PHN is classified as a neuropathic pain that is associated with mechanical allodynia where normally innocuous tactile stimuli are perceived as painful[2].In actual studies it is reported that the incidence of HZ is 3.2 to 4.1 in 1 000 person-years[4, 5].

  5. 高效液相色谱法测定斑蝥素钠维生素B6注射液中维生素B6的含量%Determination of Vitamin B6 in Disodium Cantharidinate and Vitamin B6 Injection by HPLC

    Institute of Scientific and Technical Information of China (English)

    顾秋琴

    2014-01-01

    目的:建立斑蝥酸钠维生素B6注射液中维生素B6含量测定的HPLC法。方法采用waters symmetry C18柱(150mm×4.6mm,5.0μm),流动相为0.04%戊烷磺酸钠(用冰醋酸调节pH值至3.0)-甲醇(88∶12),流速1.0ml•min-1,检测波长291nm,柱温35℃。结果维生素B6在39.33~196.6 mg•L-1的范围内线性关系良好(r=1.0000, n=5),测得平均回收率为99.8%,RSD=0.47%(n=9)。结论本方法简便,准确,专属性强。%Objective To establish an HPLC method for the determination of vitamin C and vitamin B6 in ferrous fumarate granules. Methods The HPLC column was Waters symmetry C18 (150mm×4.6mm,5.0μm);the mobile phase was 0.04% sodium heptanesulfonate solution(the pH was adjusted to 3.0 by acetic acid) - methanol(88∶12);the flow rate was 1.0mL•min-1,the detection wavelength was 291 nm , the column temperature was 35℃. Results The linear of Vitamin B6 was 39.33~196.6mg• L-1(r=1.0000),the average recovery was 99.8% with RSD=0.47%(n=6).Conclusion The method is simple,accurate and sensitive.

  6. 斑蝥酸钠维生素B6联合X射线诱导肝癌细胞HepG2凋亡%Disodium Cantharidinate and Vitamin B6 Injection in combination with radiotherapy induces apoptosis and inhibits proliferation in hepatocellular carcinoma cell line HepG2

    Institute of Scientific and Technical Information of China (English)

    赵航宇; 梁健

    2009-01-01

    目的:探讨斑蝥酸钠维生素B6(艾易舒)注射液联合放射疗法对人肝癌细胞HepG2的抑制作用.方法:采用MTT法观察不同浓度的斑螯酸钠维生素B6注射液,对HepG2生长的影响;斑蝥酸钠维生素B6作用于HepG2细胞24 h后接受不同剂量的X射线照射.克隆形成法检测放射增敏比,Annexin-V FITC/P双染法检测HepG2的早期凋亡.倒置荧光显微镜观察肝癌细胞凋亡的形态学变化.结果:斑蝥酸钠维生素B6组、放疗处理组及斑蝥酸钠维生素B6联合放疗组均对HepG2增殖产生抑制作用,与空白对照相比,斑蝥酸钠维生素B6对HepG2有明显抑制作用.并呈现浓度依赖性特点,斑蝥酸钠维生素B6浓度为5.0 mg,L、作用时间为72 h,斑蝥酸钠维生素B6对HepG2细胞的抑制率最大.斑螯酸钠维生素B6与放疗联合处理组对HepG2的抑制率明显高于单纯斑蝥酸钠维生素B6组和单纯放疗组.提示斑蝥酸钠维生素B6与X射线对HepG2的抑制效果具有协同作用.流式细胞分析显示:2.5 mg/L斑蝥酸钠维生素B6作用于HepG2细胞24 h后,11.15%的肝癌细胞发生凋亡.4 Gy的X射线作用HepG2细胞24 h,10.10%的肝癌细胞发生凋亡.5.0 mg/L斑蝥酸钠维生素B6作用于H印G2细胞24 h后对其行X射线照射,23.75%的细胞发生凋亡.克隆形成实验结果显示,斑蝥酸钠维生素B6可以增强肝癌细胞的放射敏感性.结论:斑蝥酸钠维生素B6能够显著抑制H印G2增殖,诱导HepG2凋亡,增强HepG2对X射线照射的敏感性,具有临床意义.

  7. Study on the Renal Anemia: Experimental Study in Acute Renal Anemia

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Zo Eun [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1969-09-15

    The double tracer study on erythrokinetics was carried out experimentally with radioactive iron({sup 59}Fe) and chromium ({sup 51}Cr) in rabbits. The 0.1% cantharidin solution and 1% pot. perchlomate solution was given subcutaneously to 20 rabbits respectively. 3 and 6 days after injection, the blood chemistry, urine examination, ferrokinetics and apparent half survival time of RBC were ({sup 51}Cr T-1/2) determined. Following were the results: 1) Red blood cell hematocrit and hemoglobin values were moderately reduced and B.U.N. and serum creatinine values were slightly increased in the cantharidin group, while B.U.N. and serum creatinine values were within normal limits in the pot. perchlomate group. Reticulocyte values were slightly increased in the cantharidin group, while was normal range in the pot. perchlomate group. 2) Blood chemistry finding was not significant statistically in both experimental groups, but serum iron value was moderately reduced in both group. 3) Plasma volume was unchanged in both group, but red cell volume and whole blood volume were slightly reduced in both groups. 4) Results of ferrokinetics were as follows: i) The plasma iron disappearance rate was delayed in both groups. Plasma iron turnover rate, red cell iron utilization and red cell iron turnover rate were decreased in both groups, and then red cell iron turnover rate was more decreased than plasma iron turnover rate in both groups. Circulating red cell iron was slightly increased in cantharidin group and red cell iron concentration was within normal range in both groups. ii) P.I.T.R.-R.C.I.T. value moderately increased in the cantharidin group and slightly increased in the pot. perchlomate group. Reticulocyte index, red cell iron turnover index, plasma iron turnover index and effective erythropoiesis index were wholly reduced in both groups. iii) The red cell life span was slightly shortened in the cantharidin group while was within normal range in pot. perchlomate group. The

  8. Apoptosis induced by norcantharidin in human tumor cells

    Institute of Scientific and Technical Information of China (English)

    Zhen Xiao Sun; Qing Wen Ma; Tian De Zhao; Yu Lin Wei; Guang Sheng Wang; Jia Shi Li

    2000-01-01

    @@INTRODUCTION The antitumor activity of norcantharidin (NCTD),the demethylated analogue of cantharidin, was studied in the early 1980s in China. NCTD has no side effects on urinary organs which cantharidin has shown and is easier to synthesize, and it can inhibit the proliferation of several tumor cell lines as well as transplanted tumors. Clinical trials with NCTD as a monotherapeutic agent indicated that NCTD had beneficial effects in patients with different kinds of digestive tract cancers, such as primary hepatoma,carcinomas of esophagus and gastric cancer, but no depressive effect on bone marrow cells. NCTD can increase the white blood cell count by stimulating the bone marrow and has some antagonistic effect against leukopenia caused by other agents. The exact cellular and molecular mechanisms of NCTD on tumor cells have not yet been elucidated to date[1-3].

  9. Successful treatment of florid cutaneous warts with intravenous cidofovir in an 11-year-old girl.

    OpenAIRE

    IRVINE, ALAN

    2008-01-01

    Cutaneous warts, commonly seen in children and the immunosuppressed are socially distressing and are often resistant to traditional treatments. Here, we report an 11-year-old girl with bilateral florid verrucous lesions on her hands, feet and chin, which were refractory to a number of standard treatments including cryotherapy, cantharidin preparations, topical salicylic acid, surgical debulking techniques, oral Cimetidine, and topical and intralesional Cidofovir. As the disfiguring lesions ha...

  10. Pharmacokinetics of cefetamet in plasma and skin blister fluid.

    OpenAIRE

    Zimmerli, W; Sansano, S; Wittke, B

    1996-01-01

    Cefetamet pivoxil is an oral cephalosporin with enhanced affinity for the target penicillin-binding proteins 1 and 3 and an increased stability to beta-lactamases compared with older cephalosporins, such as cefalexin or cefaclor. The pharmacokinetics of cefetamet pivoxil was determined after the seventh and final dose of 500 mg of cefetamet pivoxil in eight healthy volunteers. Concentrations in plasma and cantharidin-induced skin blister fluid were determined by a high-performance liquid chro...

  11. A Comparison of Human Neutrophils Acquired from Four Experimental Models of Inflammation

    Science.gov (United States)

    Motwani, Madhur P.; Day, Richard M.; Gilroy, Derek W.; O’Brien, Alastair J.

    2016-01-01

    Defects in neutrophil function have been implicated in a wide spectrum of clinical conditions. Several models are employed to study activated human neutrophils akin to those found at a site of inflammation. These include whole blood (WB) ex vivo stimulation with lipopolysaccharide (LPS) and in vivo techniques: cantharidin blister, skin windows and intra-dermal injection of UV-killed E.coli (UVKEc). Neutrophils obtained from these have never been compared. We compared the activation status of neutrophils from each technique in order to inform the optimal model for use in human studies. Healthy male volunteers were randomised to undergo one of the four techniques (n = 5/group). LPS: WB stimulated with 1ng/ml of LPS for 4 hours. Cantharidin: 12.5μl of 0.1% cantharidin elicited a single blister, aspirated at 24 hours. Skin windows: four 6mm mechanical-suction blisters created, de-roofed and an exudate-collection chamber placed over the windows for 4 hours before aspiration. UVKEc: 1.5 x 107 UVKEc injected intra-dermally. A single 10mm mechanical-suction blister formed and aspirated at 4 hours. Unstimulated WB used as the control. Flow cytometry was used to determine activation status using CD16, CD11b, CD54, CD62L and CD88. Functional status was assessed with a phagocytosis assay. The pattern of neutrophil activation was similar in all models. Neutrophil CD11b was elevated in all models, most markedly in UVKEc (p<0.0001), and CD54 was also elevated but only significant in the LPS model (p = 0.001). CD62L was significantly reduced in all 4 models (p<0.0001) and CD88 was also suppressed in all. There were no changes in CD16 in any model, neither was there any significant difference in the phagocytic capacity of the neutrophils. In summary, there are no significant differences in activation marker expression or phagocytic capacity in the neutrophils obtained from each technique. Therefore we believe whole blood stimulation is the best model in experimentally challenging

  12. Bugs as drugs, Part 1: Insects: the "new" alternative medicine for the 21st century?

    Science.gov (United States)

    Cherniack, E Paul

    2010-07-01

    Insects and insect-derived products have been widely used in folk healing in many parts of the world since ancient times. Promising treatments have at least preliminarily been studied experimentally. Maggots and honey have been used to heal chronic and post-surgical wounds and have been shown to be comparable to conventional dressings in numerous settings. Honey has also been applied to treat burns. Honey has been combined with beeswax in the care of several dermatologic disorders, including psoriasis, atopic dermatitis, tinea, pityriasis versicolor, and diaper dermatitis. Royal jelly has been used to treat postmenopausal symptoms. Bee and ant venom have reduced the number of swollen joints in patients with rheumatoid arthritis. Propolis, a hive sealant made by bees, has been utilized to cure aphthous stomatitis. Cantharidin, a derivative of the bodies of blister beetles, has been applied to treat warts and molluscum contagiosum. Combining insects with conventional treatments may provide further benefit.

  13. Study on Quality Standard for Xiaoliu Capsule%消瘤胶囊的质量标准研究

    Institute of Scientific and Technical Information of China (English)

    顾和亚; 周琴妹; 梁海宁; 李悦予; 刘汉清

    2012-01-01

    Objective To establish the quality standard for Xiaoliu Capsule. Methods Codonopsis Radix, Salviae miltiorahizae Radix et Rhizoma and Sparganii Rhizoma Tuber were identified by TLC; HPLC method was used to determine the content of cantharidin. Results The TLC spots were quite clear;The calibration curve of cantharidin was linear within the range of 0.097 25 ~ 0.778 mg/mL,and the regression equation was A=630.5569C - 8.62857, (r=0.9999).Conclusion The method was simple, accurate and reproducible, and could be used for the quality control of Xiaoliu Capsule.%目的建立消瘤胶囊的质量标准.方法 采用薄层色谱法鉴别处方药材党参、丹参、三棱.采用高效液相色谱测定制剂中的斑蝥素含量.结果 薄层色谱鉴别在与对照药材相应的位置上,分别显相同颜色的斑点,方法专属性强,阴性对照无干扰.含量测定,斑蝥素的回归方程为:A=630.556 9C-8.628 57,r=).9999.浓度在0.097 25~0.778 mg/mL范围内线性关系良好.消瘤胶囊中斑蝥素含量拟控制在1.5%以上.结论 本方法简便、准确、重现性好,可用于消瘤胶囊的质量控制.

  14. Norcantharidin blocks Wnt/β-catenin signaling via promoter demethylation of WIF-1 in glioma.

    Science.gov (United States)

    Xie, Dajiang; Xie, Jixi; Wan, Yingfeng; Ma, Li; Qi, Xuchen; Wang, Kun; Yang, Shuxu

    2016-04-01

    Glioma is one of the most common primary intracranial tumors, and the prognosis is poor even though much treatment management is employed. Wnt/β-catenin signaling has been reported to be associated with glioma. Norcantharidin (NCTD) is the demethylated analog of cantharidin isolated from blister beetles, and it is reported to possess anticancer activity but less nephrotoxicity than cantharidin. Accordingly, we aimed to investigate NCTD as an anti-neoplastic drug that inhibits the Wnt/β‑catenin pathway via promoter demethylation of Wnt inhibitory factor-1 (WIF-1) in glioma growth in vitro. In the present study, we report that NCTD inhibited cell proliferation, induced apoptosis and cell cycle arrest, and suppressed cell migration and invasion in vitro. Moreover, we observed that the expression levels of WIF-1 mRNA and protein in the NCTD-treated cells were increased significantly compared with these levels in the negative control (NC) cells. Promoter demethylation was observed in the NCTD‑treated cells. In contrast, aberrant methylation was observed in the NC cells. Additionally, more investigation revealed that NCTD suppressed activity of Wnt/β-catenin signaling and transcription of β-catenin/TCF-4. Furthermore, the expression of apoptosis-related proteins Bcl-2 and cleaved caspase-3 indicated significant cell apoptosis. We provide initial evidence that NCTD reactivates WIF-1 from a methylation state, and downregulates canonical Wnt/β-catenin signaling. Our findings revealed that NCTD is effective for glioma in vitro and may be a new therapeutic option in vivo. PMID:26781164

  15. Aphrodisiacs past and present: a historical review.

    Science.gov (United States)

    Sandroni, P

    2001-10-01

    The drug Viagra (sildenafil) has drawn public attention to aphrodisiacs. The search for such substances dates back millennia. Aphrodisiacs can be classified by their mode of action into 3 types: those that increase (1) libido, (2) potency, or (3) sexual pleasure. Various substances of animal and plant origin have been used in folk medicines of different cultures; some have been identified pharmacologically, allowing for understanding of their mechanisms of action. For increasing libido, ambrein, a major constituent of Ambra grisea, is used in Arab countries. This tricyclic triterpene alcohol increases the concentration of several anterior pituitary hormones and serum testosterone. Bufo toad skin and glands contain bufotenine (and other bufadienolides), a putative hallucinogenic congener of serotonin. It is the active ingredient in West Indian "love stone" and the Chinese medication chan su. The aphrodisiac properties are likely of central origin, as are the other effects of the drug. For increasing potency, Panax ginseng used in traditional Chinese medicine, works as an antioxidant by enhancing nitric oxide synthesis in the endothelium of many organs, including the corpora cavernosa; ginsenosides also enhance acetylcholine-induced and transmural nerve stimulation-activated relaxation associated with increased tissue cyclic guanosine monophosphate, hence the aphrodisiac properties. For increasing sexual pleasure, cantharidin ("Spanish fly") is a chemical with vesicant properties derived from blister beetles, which have been used for millennia as a sexual stimulant. Its mode of action is by inhibition of phosphodiesterase and protein phosphatase activity and stimulation of beta-receptors, inducing vascular congestion and inflammation. Morbidity from its abuse is significant. The ingestion of live beetles (Palembus dermestoides) in Southeast Asia and triatomids in Mexico may have a basis similar to cantharidin. It is of paramount importance for the physician to be

  16. Norcantharidin inhibits Wnt signal pathway via promoter demethylation of WIF-1 in human non-small cell lung cancer.

    Science.gov (United States)

    Xie, Junran; Zhang, Yaping; Hu, Xuming; Lv, Ran; Xiao, Dongju; Jiang, Li; Bao, Qi

    2015-05-01

    Wingless-type (Wnt) family of secreted glycoproteins is a group of signal molecules implicated in oncogenesis. Abnormal activation of Wnt signal pathway is associated with a variety of human cancers, including non-small cell lung cancer (NSCLC). Wnt antagonists, such as the secreted frizzled-related protein (SFRP) family, Wnt inhibitory factor-1 (WIF-1) and cerberus, inhibit Wnt signal pathway by directly binding to Wnt molecules. Norcantharidin (NCTD) is known to possess anticancer activity but less nephrotoxicity than cantharidin. In this study, we found that NCTD inhibited cell proliferation, induced apoptosis, arrested cell cycle and suppressed cell invasion/migration in vitro. Additionally, Wnt signal pathway transcription was also suppressed. NCTD treatment blocked cytoplasmic translocation of beta-catenin into the nucleus. Alterations of apoptosis-related proteins, such as Bax, cleaved caspase-3 (pro-apoptotic) and Bcl-2 (anti-apoptotic), had been detected. Furthermore, the expression levels of WIF-1 and SFRP1 were significantly increased in NCTD-treated groups compared with negative control (NC) groups. Abnormal methylation was observed in NC groups, while NCTD treatment promoted WIF-1 demethylation. The present study revealed that NCTD activated WIF-1 via promoter demethylation, inhibiting the canonical Wnt signal pathway in NSCLC, which may present a new therapeutic target in vivo. PMID:25814287

  17. Serine/threonine phosphatases in socioeconomically important parasitic nematodes--prospects as novel drug targets?

    Science.gov (United States)

    Campbell, Bronwyn E; Hofmann, Andreas; McCluskey, Adam; Gasser, Robin B

    2011-01-01

    Little is known about the fundamental biology of parasitic nematodes (=roundworms) that cause serious diseases, affecting literally billions of animals and humans worldwide. Unlocking the biology of these neglected pathogens using modern technologies will yield crucial and profound knowledge of their molecular biology, and could lead to new treatment and control strategies. Supported by studies in the free-living nematode, Caenorhabditis elegans, some recent investigations have provided improved insights into selected protein phosphatases (PPs) of economically important parasitic nematodes (Strongylida). In the present article, we review this progress and assess the potential of serine/threonine phosphatase (STP) genes and/or their products as targets for new nematocidal drugs. Current information indicates that some small molecules, known to specifically inhibit PPs, might be developed as nematocides. For instance, some cantharidin analogues are known to display exquisite PP-inhibitor activity, which indicates that some of them could be designed and tailored to specifically inhibit selected STPs of nematodes. This information provides prospects for the discovery of an entirely novel class of nematocides, which is of paramount importance, given the serious problems linked to anthelmintic resistance in parasitic nematode populations of livestock, and has the potential to lead to significant biotechnological outcomes. PMID:20732402

  18. Focus on Poisonous and Harmful Substances in Children’s Cosmetics and Improvement of Their Quality%关注有毒有害物质,提升儿童化妆品质量

    Institute of Scientific and Technical Information of China (English)

    党华; 谢文缄; 贾芳

    2016-01-01

    The quality of children' s cosmetics in China were studied by detecting the poisonous and harmful substances, including heavy metal, microorganism, methanol, formaldehyde and paraformaldehyde, boric acid and borate, salicylic acid, cantharidin, dioxane in 303 batches of commercial children' s cosmetics. Also, the suggestion of production, supervision and purchase of such kind of products were proposed.%通过对303批次儿童化妆品中重金属、微生物、甲醇、甲醛和多聚甲醛、硼酸和硼酸盐、水杨酸、斑螯素、二噁烷等有毒有害物质的检测,摸查我国儿童化妆品的质量状况,剖析问题儿童化妆品的危害,并针对性地提出生产、监管及消费建议。

  19. The temporal and spatial pattern of histone H3 phosphorylation at serine 28 and serine 10 is similar in plants but differs between mono- and polycentric chromosomes.

    Science.gov (United States)

    Gernand, D; Demidov, D; Houben, A

    2003-01-01

    Immunolabeling using site-specific antibodies against phosphorylated histone H3 at serine 10 or serine 28 revealed in plants an almost similar temporal and spatial pattern of both post-translational modification sites at mitosis and meiosis. During the first meiotic division the entire chromosomes are highly H3 phosphorylated. In the second meiotic division, like in mitosis, the chromosomes contain high phosphorylation levels in the pericentromeric region and very little H3 phosphorylation along the arms of monocentric species. In the polycentric plant Luzula luzuloides phosphorylation at both serine positions occurs along the whole chromosomes, whereas in monocentric species, only the pericentromeric regions showed strong signals from mitotic prophase to telophase. No phosphorylated serine 10 or serine 28 was detectable on single chromatids at anaphase II resulting from equational segregation of rye B chromosome univalents during the preceding anaphase I. In addition, we found a high level of serine 28 as well as of serine 10 phosphorylation along the entire mitotic monocentric chromosomes after treatment of mitotic cells using the phosphatase inhibitor cantharidin. These observations suggest that histone H3 phosphorylation at serine 10 and 28 is an evolutionarily conserved event and both sites are likely to be involved in the same process, such as sister chromatid cohesion. PMID:14610360

  20. Tattoo removal.

    Science.gov (United States)

    Adatto, Maurice A; Halachmi, Shlomit; Lapidoth, Moshe

    2011-01-01

    Over 50,000 new tattoos are placed each year in the United States. Studies estimate that 24% of American college students have tattoos and 10% of male American adults have a tattoo. The rising popularity of tattoos has spurred a corresponding increase in tattoo removal. Not all tattoos are placed intentionally or for aesthetic reasons though. Traumatic tattoos due to unintentional penetration of exogenous pigments can also occur, as well as the placement of medical tattoos to mark treatment boundaries, for example in radiation therapy. Protocols for tattoo removal have evolved over history. The first evidence of tattoo removal attempts was found in Egyptian mummies, dated to have lived 4,000 years BC. Ancient Greek writings describe tattoo removal with salt abrasion or with a paste containing cloves of white garlic mixed with Alexandrian cantharidin. With the advent of Q-switched lasers in the late 1960s, the outcomes of tattoo removal changed radically. In addition to their selective absorption by the pigment, the extremely short pulse duration of Q-switched lasers has made them the gold standard for tattoo removal.

  1. Successful treatment of florid cutaneous warts with intravenous cidofovir in an 11-year-old girl.

    Science.gov (United States)

    Cusack, Caitriona; Fitzgerald, Deborah; Clayton, Timothy M; Irvine, Alan D

    2008-01-01

    Cutaneous warts, commonly seen in children and the immunosuppressed are socially distressing and are often resistant to traditional treatments. Here, we report an 11-year-old girl with bilateral florid verrucous lesions on her hands, feet and chin, which were refractory to a number of standard treatments including cryotherapy, cantharidin preparations, topical salicylic acid, surgical debulking techniques, oral Cimetidine, and topical and intralesional Cidofovir. As the disfiguring lesions had a marked adverse effect on her quality of life, a trial of IV Cidofovir was instituted. We administered five cycles of IV Cidofovir with a 1-week interval between the first and second treatment, followed by 2-week intervals thereafter. This regime was well tolerated and we report dramatic resolution of the lesions with persistent clearance 6 months after completion of the fifth infusion. Resolution of recalcitrant warts with IV Cidofovir has been reported in a limited number of cases. Our experience supports its efficacy in this setting, and to the best of our knowledge this is the first report of successful treatment of cutaneous warts with IV Cidofovir in a pediatric case. PMID:18577053

  2. Treatment of molluscum contagiosum: a brief review and discussion of a case successfully treated with adapelene.

    Science.gov (United States)

    Scheinfeld, Noah

    2007-01-01

    Molluscum contagiosum occur in 2-8 percent of children. This infection is among the most common viral skin infections in children. Although the lesions will resolve spontaneously when puberty, there are several reasons to treat them. The lesions can be cosmetically unappealing. About 10 percent of those with this infection develop a pruritic eczematous eruption. In about 4 percent of children with molluscum, the lesions are numerous and recurrent with no other coexisting immunological problem. Patients who have atopic dermatitis may develop widespread involvement with molluscum. Treatment options include destruction, topical therapy, and oral therapy. Destructive treatment modalities include curettage, cryotherapy, expression or pricking with a sterile needle, electrodesiccation, photodynamic therapy, and laser ablation. Destructive therapy is poorly tolerated in children. Topical medical therapy includes salicylic acid, glycolic acid, tretinoin, tazortene, 5 percent sodium nitrite co-applied daily with 5 percent salicylic acid topical preparations, podofilox, liquefied phenol, tretinoin, cantharidin, and potassium hydroxide. Oral treatment of molluscum includes cimetidine. No therapy is universally effective. I report herein a case of generalized numerous and recurrent molluscum treated with minimal irritation with adapelene. PMID:18328209

  3. ROVE BEETLES (COLEOPTERA, STAPHYLINIDAE AND THEIR MEDICAL IMPORTANCE

    Directory of Open Access Journals (Sweden)

    B. Janbakhsh

    1977-06-01

    Full Text Available Rove beetle dermatitis produced by the family Staphylinidae genus Paederus has world- wide distribution some one hundred species of Paederus have been found, but it is believed that only 30 of these produce dermatitis. Up to 1976 three species of paederus have been found in Iran as: P. fusciped Curtis; P. pietschmanni Bershaner , and P. spectabilis Kraatz . Observations on the biology of Paederus SPP have shown that the greatest activity coincides with a high degree of humidity during the hot season. Some species seem to be attracted to artificial light. The most common pathological feature caused by rose beetles is a viscular dermatitis Eye lesions may occur, but they are the result of spread of the irritant with the fingers, after the insect was crushed on the skin, therefore secondary infection. Experiments have shown that dermatitis only develops when a rove beetle is crushed on the haemolymph. The vesicant substance is pederin which is distinct from cantharidin in terns of its biological, physical, and chemical propertics.

  4. Tattoo removal.

    Science.gov (United States)

    Adatto, Maurice A; Halachmi, Shlomit; Lapidoth, Moshe

    2011-01-01

    Over 50,000 new tattoos are placed each year in the United States. Studies estimate that 24% of American college students have tattoos and 10% of male American adults have a tattoo. The rising popularity of tattoos has spurred a corresponding increase in tattoo removal. Not all tattoos are placed intentionally or for aesthetic reasons though. Traumatic tattoos due to unintentional penetration of exogenous pigments can also occur, as well as the placement of medical tattoos to mark treatment boundaries, for example in radiation therapy. Protocols for tattoo removal have evolved over history. The first evidence of tattoo removal attempts was found in Egyptian mummies, dated to have lived 4,000 years BC. Ancient Greek writings describe tattoo removal with salt abrasion or with a paste containing cloves of white garlic mixed with Alexandrian cantharidin. With the advent of Q-switched lasers in the late 1960s, the outcomes of tattoo removal changed radically. In addition to their selective absorption by the pigment, the extremely short pulse duration of Q-switched lasers has made them the gold standard for tattoo removal. PMID:21865802

  5. Norcantharidin induced DU145 cell apoptosis through ROS-mediated mitochondrial dysfunction and energy depletion.

    Science.gov (United States)

    Shen, Bo; He, Pei-Jie; Shao, Chun-Lin

    2013-01-01

    Norcantharidin (NCTD), a demethylated analog of cantharidin derived from blister beetles, has attracted considerable attentions in recent years due to their definitely toxic properties and the noteworthy advantages in stimulating bone marrow and increasing the peripheral leukocytes. Hence, it is worth studying the anti-tumor effect of NCTD on human prostate cancer cells DU145. It was found that after the treatment of NCTD with different concentrations (25-100 μM), the cell proliferation was significantly inhibited, which led to the appearance of micronucleus (MN). Moreover, the cells could be killed in a dose-/time-dependent manner along with the reduction of PCNA (proliferating cell nuclear antigen) expression, destruction of mitochondrial membrane potential (MMP), down-regulation of MnSOD, induction of ROS, depletion of ATP, and activation of AMPK (Adenosine 5'-monophosphate -activated protein kinase) . In addition, a remarkable release of cytochrome c was found in the cells exposed to 100 μM NCTD and exogenous SOD-PEG could eliminate the generation of NCTD-induced MN. In conclusion, our studies indicated that NCTD could induce the collapse of MMP and mitochondria dysfunction. Accumulation of intercellular ROS could eventually switch on the apoptotic pathway by causing DNA damage and depleting ATP. PMID:24367681

  6. Norcantharidin induced DU145 cell apoptosis through ROS-mediated mitochondrial dysfunction and energy depletion.

    Directory of Open Access Journals (Sweden)

    Bo Shen

    Full Text Available Norcantharidin (NCTD, a demethylated analog of cantharidin derived from blister beetles, has attracted considerable attentions in recent years due to their definitely toxic properties and the noteworthy advantages in stimulating bone marrow and increasing the peripheral leukocytes. Hence, it is worth studying the anti-tumor effect of NCTD on human prostate cancer cells DU145. It was found that after the treatment of NCTD with different concentrations (25-100 μM, the cell proliferation was significantly inhibited, which led to the appearance of micronucleus (MN. Moreover, the cells could be killed in a dose-/time-dependent manner along with the reduction of PCNA (proliferating cell nuclear antigen expression, destruction of mitochondrial membrane potential (MMP, down-regulation of MnSOD, induction of ROS, depletion of ATP, and activation of AMPK (Adenosine 5'-monophosphate -activated protein kinase . In addition, a remarkable release of cytochrome c was found in the cells exposed to 100 μM NCTD and exogenous SOD-PEG could eliminate the generation of NCTD-induced MN. In conclusion, our studies indicated that NCTD could induce the collapse of MMP and mitochondria dysfunction. Accumulation of intercellular ROS could eventually switch on the apoptotic pathway by causing DNA damage and depleting ATP.

  7. Comparative Gene Expression Profiling of Benign and Malignant Lesions Reveals Candidate Therapeutic Compounds for Leiomyosarcoma

    Directory of Open Access Journals (Sweden)

    Badreddin Edris

    2012-01-01

    Full Text Available Leiomyosarcoma (LMS is a malignant, soft-tissue tumor for which few effective therapies exist. Previously, we showed that there are three molecular subtypes of LMS. Here, we analyzed genes differentially expressed in each of the three LMS subtypes as compared to benign leiomyomas and then used the Connectivity Map (cmap to calculate enrichment scores for the 1309 cmap drugs in order to identify candidate molecules with the potential to induce a benign, leiomyoma-like phenotype in LMS cells. 11 drugs were selected and tested for their ability to inhibit the growth of three human LMS cell lines. We identified two drugs with in vitro efficacy against LMS, one of which had a strongly negative enrichment score (Cantharidin and the other of which had a strongly positive enrichment score (MG-132. Given MG-132’s strong inhibitory effect on LMS cell viability, we hypothesized that LMS cells may be sensitive to treatment with other proteasome inhibitors and demonstrated that bortezomib, a clinically-approved proteasome inhibitor not included in the original cmap screen, potently inhibited the viability of the LMS cell lines. These findings suggest that systematically linking LMS subtype-specific expression signatures with drug-associated expression profiles represents a promising approach for the identification of new drugs for LMS.

  8. 细纹豆芫菁3-羟甲基戊二酰辅酶A-还原酶基因全长cDNA克隆及生物信息学分析%Cloning and bioinformatical analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from the blister beetle Epicauta mannerheimi ( Coleoptera: Meloidae)

    Institute of Scientific and Technical Information of China (English)

    姜鸣; 霍棠; 吕淑敏; 张雅林

    2012-01-01

    3-Hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR) is a key enzyme of the mevalonate pathway. Obtaining HMGR gene information is the basis of studying the relationship between cantharidin biosynthesis and the mevalonate pathway. In this study, a full-length cDNA of the HMGR gene was cloned from the blister beetle Epicauta mannerheimi ( Maklin) by RACE technology, which was named EmHMGR (GeneBank accession no. JQ690539). The full-length of EmHMGR is 3 118 bp with an ORF of 2 526 bp, containing a 5'-untranslated region (5'-UTR) of 178 bp and a 3'UTR of 414 bp. It encodes a deduced protein of 842-amino acid residues, with a predicted molecular mass of 92.8 kDa and isoeletric point (pI) of 6.0. Its predicted molecular formula is C4135H6604N1098O1216S50. Bioinformatical analysis showed that its instability index is 43. 37 and the GRAVY 0.091, suggesting that EmHMGR is an unstable hydrophobic protein. The deduced protein EmHMGR has the conserved functional domain of HMGR_Class I and the sterol-sensing domain, and shares more than 50% amino acid identity with HMGRs from other insects. Phylogenetic analysis showed that EmHMGR has the closest relationship with HMGRs of chrysomelids. This study provides the basis for further research on the biosynthetic pathway of cantharidin in the blister beetles.%3-羟甲基戊二酰辅酶A-还原酶(3-hydroxy-3-methylglutaryl coenzyme Areductase,HMGR)是甲羟戊酸途径的关键酶.获得芫菁体内HMGR基因信息是确定甲羟戊酸途径与斑蝥素合成相关性的基础.本研究利用RACE技术从细纹豆芫菁Epicauta mannerheimi(M(a)klin)体内克隆获得HMGR基因全长cDNA序列,命名为EmHMGR(GenBank登录号为JQ690539).该基因全长3118 bp,其中5’端非翻译区178 bp,3’端非翻译区414 bp,开放阅读框2 526 bp,编码842个氨基酸.推测的蛋白质分子量为92.8 kDa,理论等电点为6.0,预测分子式为C4135H6604N1098O1216S50,不稳定系数为43.37,总亲水性系数为0.091,为疏水性不

  9. 化癥散积颗粒对小鼠原位肝癌Bcl-2/Bax表达的影响%The effects of Huazhengsanjikeli on Bcl-2/Bax expression in mouse of primary Hepatic carcinoma

    Institute of Scientific and Technical Information of China (English)

    金学洙; 李超英; 周磊; 邢美茜; 刘铁军

    2012-01-01

    目的 通过建立小鼠原位肝癌模型,给予化癥散积颗粒灌胃,探讨其对小鼠原位肝癌Bcl-2/Bax表达的影响.方法 将H22瘤株直接注射到肝脏的方法建立小鼠原位肝癌模型;分组:模型组、阳性对照组、化癥散积颗粒高、中、低剂量组;采用TUNEL染色法检测凋亡细胞;RT-PCR和Western-Blot分别检测肿瘤组织Bcl-2和Bax mRNA和蛋白表达差异.结果 阳性对照组(又称斑蝥组)和中、高剂量组中药可以诱导肝癌细胞发生凋亡;可以显著增加肿瘤组织中Bax(P<0.05),同时Bcl-2表达与对照组相比明显降低(P<0.05),低剂量组Bcl-2和Bax表达与对照组相比无明显差异(P>0.05).结论 斑蝥组和中、高剂量组中药诱导肝癌细胞发生凋亡可能是通过抑制Bcl-2基因表达,同时增加Bax表达,从而引发一系列凋亡级联反应.%Objective To study the antitumor mechanism of Granules scattered plot of disease through the insitu-liver cancer model,in order to offer some evidence for clinical Chinese crude drug treatment. Methods Use H22 cells inject liver to build liver caner modcr; Detect apoptosis cells in tumor by TUNKL; Detect the expression difference of Bel-2/Bax by RT-PCR and Western-blot;Results The number of apoptosis cells were significantly increased in Cantharidin,medium and high dose Granules scattered plot of disease groups than in control and low dose groups; The mRNA and protein expression of Bax can be significantly up-rcgulation(P0. 05). Conclusion Cantharidin,medium and high dose Granules scattered plot of disease can induce H22 liver cancr cells to apoptosis; Granules? Scattered plot of disease can be induce liver cancer cells to apoptosis through inhibited the expression of Bcl-2 and up-regulated the expression of Bax at the same time.

  10. Studies of early effects of ultraviolet B irradiation on hairless mouse epidermis

    International Nuclear Information System (INIS)

    The present study describes various early biochemical and cell kinetic aspects of the acute response of hairless epidermis with irradiation of narrow-banded wavelengths in the ultraviolet B region of the spectrum (280-320 nm), and their possible relationship to ultraviolet carcinogenicity. In vivo exposure of hairless mouse skin to a single dose of various narrow-banded wavelengths of ultraviolet B light demonstrated that 280, 290, 297 and 302 nm had a carcinogenic potency according to the tetrazolium test. No induction of DT-diaphorase was observed, which may signify that the actions of ultraviolet B light and chemical skin carcinogens differ at the cellular level, even though the nuclear effect on DNA may in principle be the same, e.g. mutation events, activation or amplication of oncogens, inhibition of anti-oncogens, etc. The early epidermal cell kinetic after a biologically relevant dose of ultraviolet B irradiation at a wavelength of 297 nm could be divided into two periods: the initial inhibition in the uptake of tritiated thymidine and the mitotic rate were followed by a long-lasting depression in the DNA synthesis rate combined with rapid cell proliferation. This shows that the acute vascular response (erythema and edema) to ultraviolet B lights is also associated with epidermal perturbations similar to the carcinogen-associated delay in cell cycle passage seen after chemical skin carcinogens like 7,12-dimethylbenz(α)anthracene and methylnitrosourea, as well as to the regenerative proliferation observed after chemical skin irritants like cantharidin. 93 refs., 6 figs

  11. Automorphosis of higher plants in space is simulated by using a 3-dimensional clinostat or by application of chemicals

    Science.gov (United States)

    Miyamoto, K.; Hoshino, T.; Hitotsubashi, R.; Yamashita, M.; Ueda, J.

    In STS-95 space experiments, etiolated pea seedlings grown under microgravity conditions in space have shown to be automorphosis. Epicotyls were almost straight but the most oriented toward the direction far from their cotyledons with ca. 45 degrees from the vertical line as compared with that on earth. In order to know the mechanism of microgravity conditions in space to induce automorphosis, we introduced simulated microgravity conditions on a 3-dimensional clinostat, resulting in the successful induction of automorphosis-like growth and development. Kinetic studies revealed that epicotyls bent at their basal region or near cotyledonary node toward the direction far from the cotyledons with about 45 degrees in both seedlings grown on 1 g and under simulated microgravity conditions on the clinostat within 48 hrs after watering. Thereafter epicotyls grew keeping this orientation under simulated microgravity conditions on the clinostat, whereas those grown on 1 g changed the growth direction to vertical direction by negative gravitropic response. Automorphosis-like growth and development was induced by the application of auxin polar transport inhibitors (2,3,5-triiodobenzoic acid, N-(1-naphtyl)phthalamic acid, 9-hydroxyfluorene-9-carboxylic acid), but not an anti-auxin, p-chlorophenoxyisobutyric acid. Automorphosis-like epicotyl bending was also phenocopied by the application of inhibitors of stretch-activated channel, LaCl3 and GdCl3, and by the application of an inhibitor of protein kinase, cantharidin. These results suggest that automorphosis-like growth in epicotyls of etiolated pea seedlings is due to suppression of negative gravitropic responses on 1 g, and the growth and development of etiolated pea seedlings under 1 g conditions requires for normal activities of auxin polar transport and the gravisensing system relating to calcium channels. Possible mechanisms of perception and transduction of gravity signals to induce automorphosis are discussed.

  12. Application of biological pesticides in the control of codling moth, Cydia pomonella (L.)%生物源农药在苹果蠹蛾防治中的应用

    Institute of Scientific and Technical Information of China (English)

    吴正伟; 杨雪清; 张雅林

    2015-01-01

    The codling moth, Cydia pomonella (L.) (Lepidoptera:Tortricidae), a notorious quarantine fruit pest worldwide, poses a serious threat to the main apple producing areas in China. In terms of food safety, environmentally friendly biological pesticides are desirable substitutes for chemical pesticides;the long ̄term use of the latter have caused resistance, preventing its long team use for codling moth control. The present review summarized the use of biological pesticides, including parasitoids, sterilized insect, granu ̄lovirus, entomopathogenic nematodes, Bacillus thuringiensis, entomopathogenic fungi, microsporidia, sex pheromone, cantharidin, and spinosad that have been either applied or suggested to control codling moth. The challenges faced by biological pesticides are al ̄so discussed, and anyhow it will play an important role in the integrated pest management of codling moth due to its characteristics of infinite variety, wide source, good selectivity in application.%苹果蠹蛾是世界性检疫害虫,对我国苹果优势产区构成了巨大威胁。长期依赖化学防治使该虫抗性问题变得十分严峻。为了保障食品安全,以环境友好的生物源农药替代化学农药已成为当前苹果蠹蛾防治的热点。本文对国内外现有的生物源农药,如寄生蜂、不育昆虫、颗粒体病毒、病原线虫、Bt、病原真菌、微孢子虫、性信息素、斑蝥素、多杀菌素等,在苹果蠹蛾防治中的最新应用及其存在的问题进行论述,讨论了生物源农药凭借其种类多、来源广且在用药时期上选择性强等特点,在该虫综合治理中的重要地位及面临的挑战。

  13. Analysis of the adverse drug reaction of the Chinese herbal%活血化瘀中药的不良反应分析

    Institute of Scientific and Technical Information of China (English)

    白宇明; 魏国义

    2013-01-01

    目的 分析活血化瘀中药的不良反应发生情况,促进临床合理用药.方法 通过以中药“不良反应”为关键词,检索CHKD期刊全文数据中1998-2011年国内所有文献,查阅中药致不良反应文献的原文,并进行分析.结果 15种常用活血化瘀基本中药引起的12类不良反应及药源性疾病共计203例,其中累及系统主要为泌尿系统62例,呼吸系统47例,皮肤症状27例,急性中毒13例和循环系统12例.引起不良反应较多的药物为斑蝥(6种)、水蛭、丹参、马钱子(各5种),当归、川芎、乳香、三七(各3种).年龄越大,活血化瘀中草药不良反应构成比越高.女性活血化瘀中草药不良反应构成比较男性高.结论 活血化瘀中药不良反应分类繁多,临床表现多种多样,应引起医务人员和广大患者的重视.%Objective To analyze the adverse drug reaction of blood-activating and stasis-resoloing Chinese herbal medicine.And in order to promote the clinical reasonable using medicine.Methods "Adverse drug reaction" was as the key words,all literature of full text data the CHKD journal from 1998 to 2011 were retrieved,literature of adverse reactions caused by the original inspection Chinese medicine were checked.Results A total of 15 kinds of commonly used blood circulation Chinese medicine caused 12 adverse reactions and drug-induced disease was 203 cases,involving 62 cases of the urinary system,47 cases of the respiratory system,27 cases of skin symptoms,13 cases of acute poisoning and cyclesystem in 12 cases.Drugs to cause adverse reactions were cantharidin (6 species),leeches,Salvia,horse vomica (5),Angelica,Chuanxiong,frankincense,Panax (3).The older,the higher the ratio of adverse reactions of Chinese herbal medicine.The female had higher incidence than men.Conclusion Traditional Chinese medicine has adverse classification and diverse clinical manifestations.The medical staff and the majority of patients should pay more attention on

  14. 去甲斑蝥素对Burkitt淋巴瘤Raji细胞株生物学特性的影响%Norcantharidin inhibits proliferation of Burkitt lymphoma Raji cells and induces cell cycle arrest

    Institute of Scientific and Technical Information of China (English)

    余莉华; 曾雅莉; 贺艳杰; 胡亮杉; 宋朝阳; 郭坤元

    2012-01-01

    目的:探讨去甲斑蝥素(nor-cantharidin,NCTD)对Raji细胞的增殖抑制及周期阻滞作用.方法:通过台盼蓝拒染法观察NCTD对Raji细胞及外周血单个核细胞(peripheral blood mononuclear cells,PBMC)的增殖抑制情况;甲基纤维素集落形成法观察NCTD对Raji细胞自我更新能力的影响;流式细胞仪检测Raji 细胞周期变化.结果:NCTD抑制Raji细胞增殖,具有剂量时间依赖性(P < 0.05),但是对单个核细胞增殖无影响(P > 0.05);NCTD显著抑制Raji细胞的集落形成能力(P < 0.05),NCTD使 Raji细胞G2/M期细胞增多,G0/G1期细胞和S期细胞减少.结论:去甲斑蝥素能抑制Raji细胞增殖并产生G2/M期阻滞,且对Raji原始干祖细胞有抑制作用,有可能成为一种新的抗瘤制剂作用于人Burkitt淋巴瘤.%Objective To investigate the effects of Norcantharidin (NCTD) on human Burkitt lymphoma cell line Raji proliferation and cell cycle arrest in vitro. Methods Trypan blue assay was used to detect the inhibiting rate of NCTD on Raji cells and peripheral blood mononuclear cells (PBMC), in which concentration of NCTD were different. The self-renewal and proliferating ability were examined by methylcellblose conlony-forming units(CFU) assay. The cell cycle were detected by flow cytometry. Results By using Trypan blue assay, we found that NCTD can inhibit the proliferation of Raji cells significantly in dose- and time-dependant manners (P 0.05). The CFU assay shows that the amount of colony-forming number decreased more in Raji cells administated with NCTD than in Raji cells which were pre-treated with NCTD for 48 h. The proportion of cells at the G2/M stage increased while ones at the G

  15. Effects of Sodium Cantharidate Vitamin B6 on Proliferation,Apoptosis and Influence of NF-κB and Caspase3/7 on Human Lung Cancer A549 Cells%斑蝥酸钠维生素B6注射液对人肺癌细胞系A549增殖抑制及核因子κB和Caspase3/7的影响

    Institute of Scientific and Technical Information of China (English)

    温省初; 王一飞; 李爱明; 李冠军; 成志勇; 王亚丽; 石林

    2011-01-01

    Objective To investigate the effect of sodium cantharidinate ( SC ) vitamin B6 on human non - small cell lung cancer A549 cell proliferation, apoptosis and the influence of transcription factor NF - kB and apoptosis molecules Caspase3/7. Methods Different concentrations of SC vitamin B6 and A549 cells were cultured together; Cells apoptosis was tested by light microscopy and fluorescent staining Hoechst33342 morphology; MTT assay tested cell proliferation; Rhodamine 123 examined mitochondrial membrane potential; Caspase3/7 activity assay kit tested Caspase3/7 activity; Western blot detected of NF - kB P65 , I - kB protein levels. Results SC vitamin B6 inhibited the A549 cells proliferation, of which there were apparent apoptotic morphological changes. When 5. 0 mg/L group roled in A549 cells 72 h, cell proliferation inhibition rate reached 67. 37 percent maximum. Mitochondrial membrane potential results showed that with increasing concentration of SC vitamin B6 and time, the mitochondrial membrane potential gradually weakened, while Caspase3/7 protein activity increased. After SC vitamin B6 was added in A549 cells, NF - kB P65 protein levels was reduced ( P < 0. 05 ) and I - kB protein levels had no changes. Conclusion SC vitamin B6 inhibits the NF - kB P65 expression, activates caspase - 3/7 activities which inhibits A549 cells proliferation and induce apoptosis.%目的 探讨斑蝥酸钠(SC)维生素B6注射液对人非小细胞肺癌A549细胞增殖、凋亡及核因子κB(NF-κB)、凋亡分子Caspase3/7的影响.方法 用不同浓度(0、1.0、2.5、5.0 mg/L)的SC维生素B6注射液处理A549细胞,观察光镜及Hoechst33342荧光染色检测细胞凋亡形态;用噻唑蓝(MTT)比色法检测SC维生素B6注射液对细胞增殖的抑制作用;罗丹明123检测线粒体膜电位;Caspase3/7活性检测试剂盒检测Caspase3/7活性;蛋白印迹检测NF-κB P65、I-κB 蛋白表达.结果 SC维生素B6注射液对A549细胞的体外增殖有明显抑制作