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Sample records for bright green-emitting fluorescent

  1. Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis

    International Nuclear Information System (INIS)

    Ebisawa, Tatsuki; Yamamura, Akihiro; Kameda, Yasuhiro; Hayakawa, Kou; Nagata, Koji; Tanokura, Masaru

    2009-01-01

    A monomeric mutant of Azami-Green from G. fascicularis was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal belonged to space group P1 and diffracted X-rays to 2.20 Å resolution. Monomeric Azami-Green (mAG) from the stony coral Galaxea fascicularis is the first monomeric green-emitting fluorescent protein that is not a derivative of Aequorea victoria green fluorescent protein (avGFP). mAG and avGFP are 27% identical in amino-acid sequence. Diffraction-quality crystals of recombinant mAG were obtained by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The mAG crystal diffracted X-rays to 2.20 Å resolution on beamline AR-NW12A at the Photon Factory (Tsukuba, Japan). The crystal belonged to space group P1, with unit-cell parameters a = 41.78, b = 51.72, c = 52.89 Å, α = 90.96, β = 103.41, γ = 101.79°. The Matthews coefficient (V M = 2.10 Å 3 Da −1 ) indicated that the crystal contained two mAG molecules per asymmetric unit

  2. Fluorescence brightness and photostability of individual copper (I) oxide nanocubes.

    Science.gov (United States)

    Zohora, Nafisa; Kandjani, Ahmad Esmaielzadeh; Orth, Antony; Brown, Hannah M; Hutchinson, Mark R; Gibson, Brant C

    2017-12-04

    Conventional organic fluorophores lose their ability to fluoresce after repeated exposure to excitation light due to photobleaching. Therefore, research into emerging bright and photostable nanomaterials has become of great interest for a range of applications such as bio-imaging and tracking. Among these emerging fluorophores, metal oxide-based nanomaterials have attracted significant attention as a potential multifunctional material with photocatalytic and angeogenisis abilities in addition to fluorescnce applications. However, most of these applications are highly dependent on size, morphology, and chemo-physical properties of individual particles. In this manuscript, we present a method to study the intrinsic optical characteristics of individual copper (I) oxide (Cu 2 O) nanocubes. When excited at 520 nm using only 11 µW excitation power (1.7 W/cm2), individual nanocubes were observed to emit light with peak wavelengths ~760 nm which is conveniently within the near-infrared 1 (NIR1) biological window where tissue autofluorescence is minimal. Bright and photostable fluorescence was observed with intensities up to 487 K counts/s under constant illumination for at least 2 minutes with a brightness approximately four times higher than the autofluorescence from a fixed cumulus-oocyte complex. With near-IR emission, high fluorescence brightness, and outstanding photostability, Cu 2 O nanocubes are attractive candidates for long-term fluorescent bioimaging applications.

  3. Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli.

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    Kwang-Ho Hur

    Full Text Available The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoichiometry of a labeled protein in a sample. Here we extended brightness analysis, which has been mainly applied in eukaryotic cells, to prokaryotic cells with E. coli serving as a model system. The small size of the E. coli cell introduces unique challenges for applying brightness analysis that are addressed in this work. Photobleaching leads to a depletion of fluorophores and a reduction of the brightness of protein complexes. In addition, the E. coli cell and the point spread function of the instrument only partially overlap, which influences intensity fluctuations. To address these challenges we developed MSQ analysis, which is based on the mean Q-value of segmented photon count data, and combined it with the analysis of axial scans through the E. coli cell. The MSQ method recovers brightness, concentration, and diffusion time of soluble proteins in E. coli. We applied MSQ to measure the brightness of EGFP in E. coli and compared it to solution measurements. We further used MSQ analysis to determine the oligomeric state of nuclear transport factor 2 labeled with EGFP expressed in E. coli cells. The results obtained demonstrate the feasibility of quantifying the stoichiometry of proteins by brightness analysis in a prokaryotic cell.

  4. Bright and photostable nitrogen-vacancy fluorescence from unprocessed detonation nanodiamond.

    Science.gov (United States)

    Reineck, P; Capelli, M; Lau, D W M; Jeske, J; Field, M R; Ohshima, T; Greentree, A D; Gibson, B C

    2017-01-05

    Bright and photostable fluorescence from nitrogen-vacancy (NV) centers is demonstrated in unprocessed detonation nanodiamond particle aggregates. The optical properties of these particles is analyzed using confocal fluorescence microscopy and spectroscopy, time resolved fluorescence decay measurements, and optically detected magnetic resonance experiments. Two particle populations with distinct optical properties are identified and compared to high-pressure high-temperature (HPHT) fluorescent nanodiamonds. We find that the brightness of one detonation nanodiamond particle population is on the same order as that of highly processed fluorescent 100 nm HPHT nanodiamonds. Our results may open the path to a simple and up-scalable route for the production of fluorescent NV nanodiamonds for use in bioimaging applications.

  5. Near UV-Blue Excitable Green-Emitting Nanocrystalline Oxide

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    C. E. Rodríguez-García

    2011-01-01

    Full Text Available Green-emitting Eu-activated powders were produced by a two-stage method consisting of pressure-assisted combustion synthesis and postannealing in ammonia. The as-synthesized powders exhibited a red photoluminescence (PL peak located at =616 nm when excited with =395 nm UV. This emission peak corresponds to the 5D0→7F2 transition in Eu3+. After annealing in ammonia, the PL emission changed to an intense broad-band peak centered at =500 nm, most likely produced by 4f65d1→4f7 electronic transitions in Eu2+. This green-emitting phosphor has excitation band in the near UV-blue region (=300–450 nm. X-ray diffraction analysis reveals mainly the orthorhombic EuAlO3 and Al2O3 phases. Transmission electron microscopy observations showed that the grains are formed by faceted nanocrystals (~4 nm of polygonal shape. The excellent excitation and emission properties make these powders very promising to be used as phosphors in UV solid-state diodes coupled to activate white-emitting lamps.

  6. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.

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    David F Gruber

    Full Text Available We report the identification and characterization of two new members of a family of bilirubin-inducible fluorescent proteins (FPs from marine chlopsid eels and demonstrate a key region of the sequence that serves as an evolutionary switch from non-fluorescent to fluorescent fatty acid-binding proteins (FABPs. Using transcriptomic analysis of two species of brightly fluorescent Kaupichthys eels (Kaupichthys hyoproroides and Kaupichthys n. sp., two new FPs were identified, cloned and characterized (Chlopsid FP I and Chlopsid FP II. We then performed phylogenetic analysis on 210 FABPs, spanning 16 vertebrate orders, and including 163 vertebrate taxa. We show that the fluorescent FPs diverged as a protein family and are the sister group to brain FABPs. Our results indicate that the evolution of this family involved at least three gene duplication events. We show that fluorescent FABPs possess a unique, conserved tripeptide Gly-Pro-Pro sequence motif, which is not found in non-fluorescent fatty acid binding proteins. This motif arose from a duplication event of the FABP brain isoforms and was under strong purifying selection, leading to the classification of this new FP family. Residues adjacent to the motif are under strong positive selection, suggesting a further refinement of the eel protein's fluorescent properties. We present a phylogenetic reconstruction of this emerging FP family and describe additional fluorescent FABP members from groups of distantly related eels. The elucidation of this class of fish FPs with diverse properties provides new templates for the development of protein-based fluorescent tools. The evolutionary adaptation from fatty acid-binding proteins to fluorescent fatty acid-binding proteins raises intrigue as to the functional role of bright green fluorescence in this cryptic genus of reclusive eels that inhabit a blue, nearly monochromatic, marine environment.

  7. Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants

    Directory of Open Access Journals (Sweden)

    Mann David GJ

    2012-05-01

    Full Text Available Abstract Background The expression of fluorescent protein (FP genes as real-time visual markers, both transiently and stably, has revolutionized plant biotechnology. A palette of colors of FPs is now available for use, but the diversity has generally been underutilized in plant biotechnology. Because of the green and far-red autofluorescent properties of many plant tissues and the FPs themselves, red and orange FPs (RFPs, and OFPs, respectfully appear to be the colors with maximum utility in plant biotechnology. Within the color palette OFPs have emerged as the brightest FP markers in the visible spectra. This study compares several native, near-native and modified OFPs for their “brightness” and fluorescence, therefore, their usability as marker genes in transgenic plant tissues. Results The OFPs DsRed2, tdTomato, mOrange and pporRFP were all expressed under the control of the CaMV 35S promoter in agroinfiltration-mediated transient assays in Nicotiana benthamiana. Each of these, as well as endoplasmic reticulum (ER-targeted versions, were stably expressed in transgenic Nicotiana tabacum and Arabidopsis thaliana. Congruent results were observed between transient and stable assays. Our results demonstrated that there are several adequate OFP genes available for plant transformation, including the new pporRFP, an unaltered tetramer from the hard coral Porites porites. When the tandem dimer tdTomato and the monomeric mOrange were targeted to the ER, dramatic, ca. 3-fold, increase in plant fluorescence was observed. Conclusions From our empirical data, and a search of the literature, it appears that tdTomato-ER and mOrange-ER are the two highest fluorescing FPs available as reporters for transgenic plants. The pporRFP is a brightly fluorescing tetramer, but all tetramer FPs are far less bright than the ER-targeted monomers we report here.

  8. Multistage morphological segmentation of bright-field and fluorescent microscopy images

    Science.gov (United States)

    Korzyńska, A.; Iwanowski, M.

    2012-06-01

    This paper describes the multistage morphological segmentation method (MSMA) for microscopic cell images. The proposed method enables us to study the cell behaviour by using a sequence of two types of microscopic images: bright field images and/or fluorescent images. The proposed method is based on two types of information: the cell texture coming from the bright field images and intensity of light emission, done by fluorescent markers. The method is dedicated to the image sequences segmentation and it is based on mathematical morphology methods supported by other image processing techniques. The method allows for detecting cells in image independently from a degree of their flattening and from presenting structures which produce the texture. It makes use of some synergic information from the fluorescent light emission image as the support information. The MSMA method has been applied to images acquired during the experiments on neural stem cells as well as to artificial images. In order to validate the method, two types of errors have been considered: the error of cell area detection and the error of cell position using artificial images as the "gold standard".

  9. Ultra-fast bright field and fluorescence imaging of the dynamics of micrometer-sized objects

    Science.gov (United States)

    Chen, Xucai; Wang, Jianjun; Versluis, Michel; de Jong, Nico; Villanueva, Flordeliza S.

    2013-06-01

    High speed imaging has application in a wide area of industry and scientific research. In medical research, high speed imaging has the potential to reveal insight into mechanisms of action of various therapeutic interventions. Examples include ultrasound assisted thrombolysis, drug delivery, and gene therapy. Visual observation of the ultrasound, microbubble, and biological cell interaction may help the understanding of the dynamic behavior of microbubbles and may eventually lead to better design of such delivery systems. We present the development of a high speed bright field and fluorescence imaging system that incorporates external mechanical waves such as ultrasound. Through collaborative design and contract manufacturing, a high speed imaging system has been successfully developed at the University of Pittsburgh Medical Center. We named the system "UPMC Cam," to refer to the integrated imaging system that includes the multi-frame camera and its unique software control, the customized modular microscope, the customized laser delivery system, its auxiliary ultrasound generator, and the combined ultrasound and optical imaging chamber for in vitro and in vivo observations. This system is capable of imaging microscopic bright field and fluorescence movies at 25 × 106 frames per second for 128 frames, with a frame size of 920 × 616 pixels. Example images of microbubble under ultrasound are shown to demonstrate the potential application of the system.

  10. Green-emitting MADF complex for OLED applications

    Science.gov (United States)

    Klimes, Kody; Zhu, Zhi-Qiang; Holloway, Sean; Li, Jian

    2016-09-01

    In this article, we demonstrated an exceptional palladium complex that exhibits both phosphorescence and delayed fluorescence for use as an efficient emitter in OLEDs. Devices employing PdN3N achieved external quantum efficiencies in excess of 22% and remarkable device operational lifetime to 90% initial luminance estimated at over 30,000 h at a practical luminance of 100 cd/m2. Further tuning of the phosphorescent and delayed fluorescent emission should have a great impact in the development of efficient and stable emitters for deep blue or white OLEDs.

  11. A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest.

    Science.gov (United States)

    Dahlberg, Peter D; Boughter, Christopher T; Faruk, Nabil F; Hong, Lu; Koh, Young Hoon; Reyer, Matthew A; Shaiber, Alon; Sherani, Aiman; Zhang, Jiacheng; Jureller, Justin E; Hammond, Adam T

    2016-11-01

    A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples. First, the instrument is used to resolve three differently labeled fluorescent beads in vitro. Second, the instrument is used to recover time dependent bleaching dynamics that have distinct spectral changes in the cyanobacteria, Synechococcus leopoliensis UTEX 625. Lastly, the technique is used to acquire the absorption spectra of CH 3 NH 3 PbBr 3 perovskites and measure differences between nanocrystal films and micron scale crystals.

  12. A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest

    Science.gov (United States)

    Dahlberg, Peter D.; Boughter, Christopher T.; Faruk, Nabil F.; Hong, Lu; Koh, Young Hoon; Reyer, Matthew A.; Shaiber, Alon; Sherani, Aiman; Zhang, Jiacheng; Jureller, Justin E.; Hammond, Adam T.

    2016-11-01

    A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples. First, the instrument is used to resolve three differently labeled fluorescent beads in vitro. Second, the instrument is used to recover time dependent bleaching dynamics that have distinct spectral changes in the cyanobacteria, Synechococcus leopoliensis UTEX 625. Lastly, the technique is used to acquire the absorption spectra of CH3NH3PbBr3 perovskites and measure differences between nanocrystal films and micron scale crystals.

  13. A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest

    OpenAIRE

    Dahlberg, Peter D.; Boughter, Christopher T.; Faruk, Nabil F.; Hong, Lu; Koh, Young Hoon; Reyer, Matthew A.; Shaiber, Alon; Sherani, Aiman; Zhang, Jiacheng; Jureller, Justin E.; Hammond, Adam T.

    2016-01-01

    A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. Th...

  14. Preparation of Au Nanoclusters-Modified Polylactic Acid Fiber with Bright Red Fluorescence and its Use as Sensing Probe.

    Science.gov (United States)

    Zhu, Wenli; Li, Huili; Wan, Ajun; Liu, Lanbo

    2017-01-01

    In present work, the Au nanoclusters-modified polylactic acid fiber (PLA-Au NCs) with bright red fluorescence were fabricated by the encapsulation of Au nanoclusters (Au NCs) in the PLA fiber treated with H 2 O 2 . The Au 25 nanoclusters stabilized by bovine serum albumin (BSA-Au NCs) were prepared via an improved "green" synthetic routine. With pretreatment of the PLA fiber in H 2 O 2 concentration of 12 and 18 %, the as-prepared PLA-Au NCs exhibited brighter red emission with a strong peak centered at ~640 nm than BSA-Au NCs. The fluorescence can be quenched by nitric oxide (NO). A good linear relationship between the relative fluorescence quenching intensity of the as-prepared PLA-Au NCs and the concentration of NO can be obtained in the range of 0.0732 to 0.7320 mM, and the detection limit was 0.0070 mM.

  15. Innovative molecular-based fluorescent nanoparticles for multicolor single particle tracking in cells

    International Nuclear Information System (INIS)

    Daniel, Jonathan; Blanchard-Desce, Mireille; Godin, Antoine G; Palayret, Matthieu; Lounis, Brahim; Cognet, Laurent

    2016-01-01

    Based on an original molecular-based design, we present bright and photostable fluorescent organic nanoparticles (FONs) showing excellent colloidal stability in various aqueous environments. Complementary near-infrared emitting and green emitting FONs were prepared using a simple, fast and robust protocol. Both types of FONs could be simultaneously imaged at the single-particle level in solution as well as in biological environments using a monochromatic excitation and a dual-color fluorescence microscope. No evidence of acute cytotoxicity was found upon incubation of live cells with mixed solutions of FONs, and both types of nanoparticles were found internalized in the cells where their motion could be simultaneously tracked at video-rate up to minutes. These fluorescent organic nanoparticles open a novel non-toxic alternative to existing nanoparticles for imaging biological structures, compatible with live-cell experiments and specially fitted for multicolor single particle tracking. (paper)

  16. Expression profiling of Plasmodium berghei HSP70 genes for generation of bright red fluorescent parasites.

    Directory of Open Access Journals (Sweden)

    Marion Hliscs

    Full Text Available Live cell imaging of recombinant malarial parasites encoding fluorescent probes provides critical insights into parasite-host interactions and life cycle progression. In this study, we generated a red fluorescent line of the murine malarial parasite Plasmodium berghei. To allow constitutive and abundant expression of the mCherry protein we profiled expression of all members of the P. berghei heat shock protein 70 (HSP70 family. We identified PbHSP70/1, an invariant ortholog of Plasmodium falciparum HSP70-1, as the protein with the highest expression levels during Plasmodium blood, mosquito, and liver infection. Stable allelic insertion of a mCherry expression cassette into the PbHsp70/1 locus created constitutive red fluorescent P. berghei lines, termed Pbred. We show that these parasites can be used for live imaging of infected host cells and organs, including hepatocytes, erythrocytes, and whole Anopheles mosquitoes. Quantification of the fluorescence intensity of several Pbred parasite stages revealed significantly enhanced signal intensities in comparison to GFP expressed under the control of the constitutive EF1alpha promoter. We propose that systematic transcript profiling permits generation of reporter parasites, such as the Pbred lines described herein.

  17. Zwitterionic Silane Copolymer for Ultra-Stable and Bright Biomolecular Probes Based on Fluorescent Quantum Dot Nanoclusters.

    Science.gov (United States)

    Dembele, Fatimata; Tasso, Mariana; Trapiella-Alfonso, Laura; Xu, Xiangzhen; Hanafi, Mohamed; Lequeux, Nicolas; Pons, Thomas

    2017-05-31

    Fluorescent semiconductor quantum dots (QDs) exhibit several unique properties that make them suitable candidates for biomolecular sensing, including high brightness, photostability, broad excitation, and narrow emission spectra. Assembling these QDs into robust and functionalizable nanosized clusters (QD-NSCs) can provide fluorescent probes that are several orders of magnitude brighter than individual QDs, thus allowing an even greater sensitivity of detection with simplified instrumentation. However, the formation of compact, antifouling, functionalizable, and stable QD-NSCs remains a challenging task, especially for a use at ultralow concentrations for single-molecule detection. Here, we describe the development of fluorescent QD-NSCs envisioned as a tool for fast and sensitive biomolecular recognition. First, QDs were assembled into very compact 100-150 nm diameter spherical aggregates; the final QD-NSCs were obtained by growing a cross-linked silica shell around these aggregates. Hydrolytic stability in several concentration and pH conditions is a key requirement for a potential and efficient single-molecule detection tool. However, the hydrolysis of Si-O-Si bonds leads to desorption of monosilane-based surface groups at very low silica concentrations or in a slightly basic medium. Thus, we designed a novel multidentate copolymer composed of multiple silane as well as zwitterionic monomers. Coating silica beads with this multidentate copolymer provided a robust surface chemistry that was demonstrated to be stable against hydrolysis, even at low concentrations. Copolymer-coated silica beads also showed low fouling properties and high colloidal stability in saline solutions. Furthermore, incorporation of additional azido-monomers enabled easy functionalization of QD-NSCs using copper-free bio-orthogonal cyclooctyne-azide click chemistry, as demonstrated by a biotin-streptavidin affinity test.

  18. A Novel Reporter Rat Strain That Conditionally Expresses the Bright Red Fluorescent Protein tdTomato.

    Directory of Open Access Journals (Sweden)

    Hiroyuki Igarashi

    Full Text Available Despite the strength of the Cre/loxP recombination system in animal models, its application in rats trails that in mice because of the lack of relevant reporter strains. Here, we generated a floxed STOP tdTomato rat that conditionally expresses a red fluorescent protein variant (tdTomato in the presence of exogenous Cre recombinase. The tdTomato signal vividly visualizes neurons including their projection fibers and spines without any histological enhancement. In addition, a transgenic rat line (FLAME that ubiquitously expresses tdTomato was successfully established by injecting intracytoplasmic Cre mRNA into fertilized ova. Our rat reporter system will facilitate connectome studies as well as the visualization of the fine structures of genetically identified cells for long periods both in vivo and ex vivo. Furthermore, FLAME is an ideal model for organ transplantation research owing to improved traceability of cells/tissues.

  19. The structure of mAG, a monomeric mutant of the green fluorescent protein Azami-Green, reveals the structural basis of its stable green emission

    International Nuclear Information System (INIS)

    Ebisawa, Tatsuki; Yamamura, Akihiro; Kameda, Yasuhiro; Hayakawa, Kou; Nagata, Koji; Tanokura, Masaru

    2010-01-01

    The crystal structure of a monomeric mutant of Azami-Green (mAG) from G. fascicularis was determined at 2.2 Å resolution. Monomeric Azami-Green (mAG) from the stony coral Galaxea fascicularis is the first known monomeric green-emitting fluorescent protein that is not a variant of Aequorea victoria green fluorescent protein (avGFP). These two green fluorescent proteins are only 27% identical in their amino-acid sequences. mAG is more similar in its amino-acid sequence to four fluorescent proteins: Dendra2 (a green-to-red irreversibly photoconverting fluorescent protein), Dronpa (a bright-and-dark reversibly photoswitchable fluorescent protein), KikG (a tetrameric green-emitting fluorescent protein) and Kaede (another green-to-red irreversibly photoconverting fluorescent protein). To reveal the structural basis of stable green emission by mAG, the 2.2 Å crystal structure of mAG has been determined and compared with the crystal structures of avGFP, Dronpa, Dendra2, Kaede and KikG. The structural comparison revealed that the chromophore formed by Gln62-Tyr63-Gly64 (QYG) and the fixing of the conformation of the imidazole ring of His193 by hydrogen bonds and van der Waals contacts involving His193, Arg66 and Thr69 are likely to be required for the stable green emission of mAG. The crystal structure of mAG will contribute to the design and development of new monomeric fluorescent proteins with faster maturation, brighter fluorescence, improved photostability, new colours and other preferable properties as alternatives to avGFP and its variants

  20. Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO.

    Science.gov (United States)

    Chelvanayagam, D K; Beazley, L D

    1997-03-01

    The stain toluidine blue-O (tol blue), applied to sections of neural tissue, is shown to be compatible with the vivid fluorescent lipophilic neural tracers 4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide (Di-ASP), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). As with other Nissl stains, toluidine blue-O fluoresces in the red end of the spectrum but such fluorescence quenches upon binding with tissue. Moreover, progressive staining occurs at concentrations low enough to minimise any background fluorescence attributable to non-specific residence of the stain. The bright yellow Di-ASP and vivid green DiO signals are spectrally removed from the red fluorescence of toluidine blue-O. With toluidine blue-O counterstaining, Di-ASP generally offers contrast superior to that with DiI, however, the latter is improved by viewing in a polarised green bright field. Visible Di-ASP emission, although broad, peaks at a more film-sensitive region of the spectrum than that for DiI, thus reducing the photographic exposure required.

  1. A green-emitting CdSe/Poly(butyl acrylate) nanocomposite

    NARCIS (Netherlands)

    Peres, M.; Costa, L.C.; Neves, A.; Soares, M.J.; Monteiro, T.; Carvalho Esteves, de A.C.; Barros-Timmons, A.M.M.V.; Trindade, T.; Kholkin, A.; Alves, E.

    2005-01-01

    CdSe/poly(butyl acrylate) nanocomposites were synthesized by in situ miniemulsion polymerization. The hybrid nanomaterial is very stable and presents a bright green photoluminescence at 2.29 eV under ultraviolet excitation. With the excitation conditions used the intensity of the emission band keeps

  2. A potential green emitting citrate gel synthesized NaSrBO3:Tb3+ phosphor for display application

    Science.gov (United States)

    Bedyal, A. K.; Kumar, Vinay; Swart, H. C.

    2018-04-01

    A potential green emitting NaSrBO3:Tb3+ (1-9 mol%) phosphor was synthesized by a citrate gel combustion method. X-ray diffraction patterns confirmed the monoclinic phase of the phosphor. The phosphor emitted intense green emission under near-UV and electron excitation due to the characteristic transitions 5D4→7F6(488 nm),5D4→7F5(544 nm),5D4→7F4(586 nm) and 5D4→7F3(622 nm) of Tb3+ ions. The optimal molar concentration of Tb3+ ions was found to be 6 mol%, after that concentration quenching occurred. The dipole-dipole interaction was found to be accountable for energy transfer between the Tb3+ ions. X-ray photoelectron spectroscopy was carried out to analyze the chemical states of the elements and suggest that terbium was mostly presented in the (+3) valance state in the phosphor. The approximated Commission Internationale de l‧Eclairage coordinates for the PL (0.31, 0.61) and CL (0.33, 0.57) were found to be very close to the well-known green emitting phosphor. The obtained results suggest that the studied phosphor could be an ultimate choice for green emission in display applications.

  3. Observation of weak carrier localization in green emitting InGaN/GaN multi-quantum well structure

    Energy Technology Data Exchange (ETDEWEB)

    Mohanta, Antaryami; Wang, Shiang-Fu; Jang, Der-Jun, E-mail: djjang@mail.nsysu.edu.tw [Department of Physics, National Sun Yat-sen University, Kaohsiung 80424, Taiwan (China); Young, Tai-Fa [Department of Mechanical and Electromechanical Engineering, National Sun Yat-sen University, Kaohsiung 80424, Taiwan (China); Yeh, Ping-Hung; Ling, Dah-Chin [Department of Physics, Tamkang University, Tamsui Dist., New Taipei City 25137, Taiwan (China); Lee, Meng-En [Department of Physics, National Kaohsiung Normal University, Kaohsiung 80264, Taiwan (China)

    2015-04-14

    Green emitting InGaN/GaN multi-quantum well samples were investigated using transmission electron microscopy, photoluminescence (PL), and time-resolved photoluminescence (TRPL) spectroscopy. Weak carrier localization with characteristic energy of ∼12 meV due to an inhomogeneous distribution of In in the InGaN quantum (QW) layer is observed. The temperature dependence of the PL peak energy exhibits S-shape phenomenon and is comparatively discussed within the framework of the Varshni's empirical formula. The full width at half maximum of the PL emission band shows an increasing-decreasing-increasing behavior with increasing temperature arising from the localized states caused by potential fluctuations. The radiative life time, τ{sub r}, extracted from the TRPL profile shows ∼T{sup 3/2} dependence on temperature above 200 K, which confirms the absence of the effect of carrier localization at room temperature.

  4. A Comparison of the recombination efficiency in green-emitting InGaN quantum dots and quantum wells

    International Nuclear Information System (INIS)

    Park, Il-Kyu; Kwon, Min-Ki; Park, Seong-Ju

    2012-01-01

    A comparative investigation of the recombination efficiency of green-emitting InGaN quantum dots (QDs) and quantum wells (QWs) is reported in this paper. Optical investigations using temperature dependent photoluminescence (PL) results showed that the internal quantum efficiency of InGaN QDs at room temperature was 8.7 times larger than that found for InGaN QWs because they provided dislocation-free recombination sites for the electrical charge carriers. The excitation power-dependent PL and electroluminescence results showed that the effect of the polarization induced electric field on the recombination process of electrical charge carriers in the QDs was negligibly small whereas it was dominant in the QWs. These results indicate that InGaN QDs are more beneficial than QWs in improving the luminescence efficiency of LEDs in the green spectral range.

  5. One-pot and ultrafast synthesis of nitrogen and phosphorus co-doped carbon dots possessing bright dual wavelength fluorescence emission

    Science.gov (United States)

    Sun, Xiangcheng; Brückner, Christian; Lei, Yu

    2015-10-01

    Very brief microwave heating of aniline, ethylene diamine, and phosphoric acid in water at ambient pressure generated nitrogen and phosphorus co-doped carbon dots (N,P-CDs) that exhibit bright dual blue (centred at 450 nm; 51% quantum yield) and green (centred at 510 nm, 38% quantum yield) fluorescence emission bands. The N,P-CDs were characterized using TEM, XRD, XPS, IR, UV-vis, and fluorescence spectroscopy, demonstrating their partially crystalline carbon, partially amorphous structures, and the incorporation of O, N, and P into the carbogenic scaffold. The N,P-CDs demonstrated excitation-dependent and nearly pH-independent emission properties. The unique dual emission properties lay the foundation for the use of N,P-CDs in ratiometric sensing applications.Very brief microwave heating of aniline, ethylene diamine, and phosphoric acid in water at ambient pressure generated nitrogen and phosphorus co-doped carbon dots (N,P-CDs) that exhibit bright dual blue (centred at 450 nm; 51% quantum yield) and green (centred at 510 nm, 38% quantum yield) fluorescence emission bands. The N,P-CDs were characterized using TEM, XRD, XPS, IR, UV-vis, and fluorescence spectroscopy, demonstrating their partially crystalline carbon, partially amorphous structures, and the incorporation of O, N, and P into the carbogenic scaffold. The N,P-CDs demonstrated excitation-dependent and nearly pH-independent emission properties. The unique dual emission properties lay the foundation for the use of N,P-CDs in ratiometric sensing applications. Electronic supplementary information (ESI) available: Detailed experimental section, XRD, FTIR, explosive sensing and the applications results. See DOI: 10.1039/c5nr05549k

  6. Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging.

    Science.gov (United States)

    Chen, Bin; Feng, Guangxue; He, Bairong; Goh, Chiching; Xu, Shidang; Ramos-Ortiz, Gabriel; Aparicio-Ixta, Laura; Zhou, Jian; Ng, Laiguan; Zhao, Zujin; Liu, Bin; Tang, Ben Zhong

    2016-02-10

    Robust luminescent dyes with efficient two-photon fluorescence are highly desirable for biological imaging applications, but those suitable for organic dots fabrication are still rare because of aggregation-caused quenching. In this work, a red fluorescent silole, 2,5-bis[5-(dimesitylboranyl)thiophen-2-yl]-1-methyl-1,3,4-triphenylsilole ((MesB)2 DTTPS), is synthesized and characterized. (MesB)2 DTTPS exhibits enhanced fluorescence efficiency in nanoaggregates, indicative of aggregation-enhanced emission (AEE). The organic dots fabricated by encapsulating (MesB)2 DTTPS within lipid-PEG show red fluorescence peaking at 598 nm and a high fluorescence quantum yield of 32%. Upon excitation at 820 nm, the dots show a large two-photon absorption cross section of 3.43 × 10(5) GM, which yields a two-photon action cross section of 1.09 × 10(5) GM. These (MesB)2 DTTPS dots show good biocompatibility and are successfully applied to one-photon and two-photon fluorescence imaging of MCF-7 cells and two-photon in vivo visualization of the blood vascular of mouse muscle in a high-contrast and noninvasive manner. Moreover, the 3D blood vasculature located at the mouse ear skin with a depth of over 100 μm can also be visualized clearly, providing the spatiotemporal information about the whole blood vascular network. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. One-pot synthesis of gold nanoclusters with bright red fluorescence and good biorecognition abilities for visualization fluorescence enhancement detection of E. coli.

    Science.gov (United States)

    Liu, Jiali; Lu, Lili; Xu, Suying; Wang, Leyu

    2015-03-01

    A facile one-pot strategy was developed for the synthesis of lysozyme functionalized fluorescence gold nanoclusters (AuNCs). The lysozymes added to reduce Au(3+) ions and stabilize the AuNCs during the synthesis were coated on the AuNCs surface and retained their specific recognition ability for bacteria such as Escherichia coli (E. coli). Based on such ability, these AuNCs were specifically attached onto the surface of E. coli, which resulted in great red fluorescence enhancement. Nevertheless, the bovine serum albumin (BSA) stabilized AuNCs could not recognize E. coli and no fluorescence enhancement was observed. Upon the addition of E. coli, the red fluorescence intensity of lysozyme-AuNCs was enhanced linearly over the range of 2.4×10(4) -6.0×10(6) CFU/mL of E. coli with high sensitivity (LOD=2.0×10(4) CFU/mL, S/N=3). The visualization fluorescence evolution may enable the rapid and real-time detection of bacteria. This study may be extended to other functional proteins such as antibody, enzyme, and peptide functionalized nanoclusters while retaining the bioactivity of coating proteins and find wide applications in the fields of biochemistry and biomedicine. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Fabrication and performance of ACTFEL display devices using manganese-doped zinc germanate as a green-emitting electroluminescent layer

    International Nuclear Information System (INIS)

    Kim, Joo Han; Yoon, Kyung Ho

    2010-01-01

    Alternating-current thin-film electroluminescent (ACTFEL) display devices fabricated using manganese-doped zinc germanate (Zn 2 GeO 4 :Mn) as a green-emitting electroluminescent layer material are described. The ACTFEL display devices were fabricated with a standard bottom emission structure having a multilayer stack of thin films in the metal/semiconductor/insulator/ metal (MSIM) configuration. The device was constructed on a transparent Corning glass substrate through which the emitted EL light passed. The Zn 2 GeO 4 :Mn emission layer was synthesized by using a RF magnetron sputter deposition method, followed by post-annealing at 700 .deg. C in air ambient for 1 hour. The obtained Zn 2 GeO 4 :Mn films were found to be polycrystalline with a rhombohedral crystal structure. A green emission spectrum with a maximum at approximately 538 nm was produced from the fabricated device. The chromaticity color coordinates of the EL emission were measured to be x = 0.308 and y = 0.657. The device demonstrated a sharp increase in the intensity of green EL emission upon increasing the AC peak voltage applied to the device above a threshold of 148 V.

  9. Bright blue-shifted fluorescent proteins with Cys in the GAF domain engineered from bacterial phytochromes: fluorescence mechanisms and excited-state dynamics

    NARCIS (Netherlands)

    Hontani, Yusaku; Shcherbakova, Daria M.; Baloban, Mikhail; Zhu, Jingyi; Verkhusha, Vladislav V.; Kennis, John T. M.

    2016-01-01

    Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are of great interest for in vivo imaging. They utilize biliverdin (BV) as a chromophore, which is a heme degradation product, and therefore they are straightforward to use in mammalian tissues. Here, we

  10. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

    OpenAIRE

    Bajar, Bryce T.; Wang, Emily S.; Lam, Amy J.; Kim, Bongjae B.; Jacobs, Conor L.; Howe, Elizabeth S.; Davidson, Michael W.; Lin, Michael Z.; Chu, Jun

    2016-01-01

    Many genetically encoded biosensors use F?rster resonance energy transfer (FRET) to dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent proteins (FPs) are most commonly used as FRET partner fluorophores, respectively, green and red FPs offer distinct advantages for FRET, such as greater spectral separation, less phototoxicity, and lower autofluorescence. We previously developed the green-red FRET pair Clover and mRuby2, which improves responsiveness in intra...

  11. Spatially Controlled Fabrication of Brightly Fluorescent Nanodiamond-Array with Enhanced Far-Red Si-V Luminescence

    Science.gov (United States)

    Singh, Sonal; Thomas, Vinoy; Martyshkin, Dmitry; Kozlovskaya, Veronika; Kharlampieva, Eugenia

    2014-01-01

    We demonstrate a novel approach to precise pattern fluorescent nanodiamond-arrays with enhanced far-red intense photostable luminescence from silicon-vacancy (Si-V) defect centers. The precision-patterned pre-growth seeding of nanodiamonds is achieved by scanning probe “Dip-Pen” nanolithography technique using electrostatically-driven transfer of nanodiamonds from “inked” cantilevers to a UV-treated hydrophilic SiO2 substrate. The enhanced emission from nanodiamond-dots in the far-red is achieved by incorporating Si-V defect centers in subsequent chemical vapor deposition treatment. The development of a suitable nanodiamond ink, mechanism of ink transport, and effect of humidity, dwell time on nanodiamond patterning are investigated. The precision-patterning of as-printed (pre-CVD) arrays with dot diameter and dot height as small as 735 nm ± 27 nm, 61 nm ± 3 nm, respectively and CVD-treated fluorescent ND-arrays with consistently patterned dots having diameter and height as small as 820 nm ± 20 nm, 245 nm ± 23 nm, respectively using 1 s dwell time and 30% RH is successfully achieved. We anticipate that the far-red intense photostable luminescence (~738 nm) observed from Si-V defect centers integrated in spatially arranged nanodiamonds could be beneficial for the development of the next generation fluorescent based devices and applications. PMID:24394286

  12. Spatially controlled fabrication of a bright fluorescent nanodiamond-array with enhanced far-red Si-V luminescence.

    Science.gov (United States)

    Singh, Sonal; Thomas, Vinoy; Martyshkin, Dmitry; Kozlovskaya, Veronika; Kharlampieva, Eugenia; Catledge, Shane A

    2014-01-31

    We demonstrate a novel approach to precisely pattern fluorescent nanodiamond-arrays with enhanced far-red intense photostable luminescence from silicon-vacancy (Si-V) defect centers. The precision-patterned pre-growth seeding of nanodiamonds is achieved by a scanning probe 'dip-pen' nanolithography technique using electrostatically driven transfer of nanodiamonds from 'inked' cantilevers to a UV-treated hydrophilic SiO2 substrate. The enhanced emission from nanodiamond dots in the far-red is achieved by incorporating Si-V defect centers in a subsequent chemical vapor deposition treatment. The development of a suitable nanodiamond ink and mechanism of ink transport, and the effect of humidity and dwell time on nanodiamond patterning are investigated. The precision patterning of as-printed (pre-CVD) arrays with dot diameter and dot height as small as 735 nm ± 27 nm and 61 nm ± 3 nm, respectively, and CVD-treated fluorescent ND-arrays with consistently patterned dots having diameter and height as small as 820 nm ± 20 nm and, 245 nm ± 23 nm, respectively, using 1 s dwell time and 30% RH is successfully achieved. We anticipate that the far-red intense photostable luminescence (~738 nm) observed from Si-V defect centers integrated in spatially arranged nanodiamonds could be beneficial for the development of next generation fluorescence-based devices and applications.

  13. Novel green-emitting Na2CaPO4F:Eu2+ phosphors for near-ultraviolet white light-emitting diodes

    International Nuclear Information System (INIS)

    Huang, Chien-Hao; Chen, Yen-Chi; Kuo, Te-Wen; Chen, Teng-Ming

    2011-01-01

    In this study, green-emitting Na 2 CaPO 4 F:Eu 2+ phosphors were synthesized by solid-state reactions. The excitation spectra of the phosphors showed a broad hump between 250 and 450 nm; the spectra match well with the near-ultraviolet (NUV) emission spectra of light-emitting diodes (LEDs). The emission spectrum showed an intense broad emission band centered at 506 nm. White LEDs were fabricated by integrating a 390 nm NUV chip comprising blue-emitting BaMgAl 10 O 17 :Eu 2+ , green-emitting Na 2 CaPO 4 F:0.02 Eu 2+ , and red-emitting CaAlSiN 3 :Eu 2+ phosphors into a single package; the white LEDs exhibited white light with a correlated color temperature of 5540 K, a color-rendering index of 90.75, and color coordinates (0.332, 0.365) close to those of ideal white light. - Highlights: → Novel green-emitting Na 2 CaPO 4 F:Eu 2+ phosphors were synthesized by solid-state reactions in this research. → White LEDs were fabricated by integrating a 390 nm NUV chip comprising blue-emitting BaMgAl 10 O 17 :Eu 2+ , green-emitting Na 2 CaPO 4 F:0.02Eu 2+ , and red-emitting CaAlSiN 3 :Eu 2+ phosphors into a single package. → The white LEDs exhibited white light with a correlated color temperature of 5540 K, a color-rendering index of 90.75, and color coordinates (0.332, 0.365) close to those of ideal white light.

  14. Spatially controlled fabrication of a bright fluorescent nanodiamond-array with enhanced far-red Si-V luminescence

    International Nuclear Information System (INIS)

    Singh, Sonal; Thomas, Vinoy; Kharlampieva, Eugenia; Catledge, Shane A; Martyshkin, Dmitry; Kozlovskaya, Veronika

    2014-01-01

    We demonstrate a novel approach to precisely pattern fluorescent nanodiamond-arrays with enhanced far-red intense photostable luminescence from silicon-vacancy (Si-V) defect centers. The precision-patterned pre-growth seeding of nanodiamonds is achieved by a scanning probe ‘dip-pen’ nanolithography technique using electrostatically driven transfer of nanodiamonds from ‘inked’ cantilevers to a UV-treated hydrophilic SiO 2 substrate. The enhanced emission from nanodiamond dots in the far-red is achieved by incorporating Si-V defect centers in a subsequent chemical vapor deposition treatment. The development of a suitable nanodiamond ink and mechanism of ink transport, and the effect of humidity and dwell time on nanodiamond patterning are investigated. The precision patterning of as-printed (pre-CVD) arrays with dot diameter and dot height as small as 735 nm ± 27 nm and 61 nm ± 3 nm, respectively, and CVD-treated fluorescent ND-arrays with consistently patterned dots having diameter and height as small as 820 nm ± 20 nm and, 245 nm ± 23 nm, respectively, using 1 s dwell time and 30% RH is successfully achieved. We anticipate that the far-red intense photostable luminescence (∼738 nm) observed from Si-V defect centers integrated in spatially arranged nanodiamonds could be beneficial for the development of next generation fluorescence-based devices and applications. (paper)

  15. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting.

    Science.gov (United States)

    Bajar, Bryce T; Wang, Emily S; Lam, Amy J; Kim, Bongjae B; Jacobs, Conor L; Howe, Elizabeth S; Davidson, Michael W; Lin, Michael Z; Chu, Jun

    2016-02-16

    Many genetically encoded biosensors use Förster resonance energy transfer (FRET) to dynamically report biomolecular activities. While pairs of cyan and yellow fluorescent proteins (FPs) are most commonly used as FRET partner fluorophores, respectively, green and red FPs offer distinct advantages for FRET, such as greater spectral separation, less phototoxicity, and lower autofluorescence. We previously developed the green-red FRET pair Clover and mRuby2, which improves responsiveness in intramolecular FRET reporters with different designs. Here we report the engineering of brighter and more photostable variants, mClover3 and mRuby3. mClover3 improves photostability by 60% and mRuby3 by 200% over the previous generation of fluorophores. Notably, mRuby3 is also 35% brighter than mRuby2, making it both the brightest and most photostable monomeric red FP yet characterized. Furthermore, we developed a standardized methodology for assessing FP performance in mammalian cells as stand-alone markers and as FRET partners. We found that mClover3 or mRuby3 expression in mammalian cells provides the highest fluorescence signals of all jellyfish GFP or coral RFP derivatives, respectively. Finally, using mClover3 and mRuby3, we engineered an improved version of the CaMKIIα reporter Camuiα with a larger response amplitude.

  16. Femtogram-level detection of Clostridium botulinum neurotoxin type A by sandwich immunoassay using nanoporous substrate and ultra-bright fluorescent suprananoparticles.

    Science.gov (United States)

    Bok, Sangho; Korampally, Venumadhav; Darr, Charles M; Folk, William R; Polo-Parada, Luis; Gangopadhyay, Keshab; Gangopadhyay, Shubhra

    2013-03-15

    We report a simple, robust fluorescence biosensor for the ultra-sensitive detection of Clostridium botulinum Neurotoxin Type A (BoNT/A) in complex, real-world media. High intrinsic signal amplification was achieved through the combined use of ultra-bright, photostable dye-doped nanoparticle (DOSNP) tags and high surface area nanoporous organosilicate (NPO) thin films. DOSNP with 22 nm diameter were synthesized with more than 200 times equivalent free dye fluorescence and conjugated to antibodies with average degree of substitution of 90 dyes per antibody, representing an order of magnitude increase compared with conventional dye-labeled antibodies. The NPO films were engineered to form constructive interference at the surface where fluorophores were located. In addition, DOSNP-labeled antibodies with NPO films increased surface roughness causing diffuse scattering resulting in 24% more scattering intensity than dye-labeled antibody with NPO films. These substrates were used for immobilization of capture antibodies against BoNT/A, which was further quantified by DOSNP-labeled signal antibodies. The combination of optical effects enhanced the fluorescence and, therefore, the signal-to-noise ratio significantly. BoNT/A was detected in PBS buffer down to 21.3 fg mL(-1) in 4 h. The assay was then extended to several complex media and the four-hour detection limit was found to be 145.8 fg mL(-1) in orange juice and 164.2 fg mL(-1) in tap water, respectively, demonstrating at least two orders of magnitude improvement comparing to the reported detection limit of other enzyme-linked immunosorbent assays (ELISA). This assay, therefore, demonstrates a novel method for rapid, ultra-low level detection of not only BoNT/A, but other analytes as well. Copyright © 2012 Elsevier B.V. All rights reserved.

  17. Femtosecond fluorescence upconversion spectroscopy of vapor-deposited tris(8-hydroxyquinoline) aluminum films.

    NARCIS (Netherlands)

    Humbs, W.; Zhang, H.; Glasbeek, M.

    2000-01-01

    Abstract Vapor-deposited Alq3 is used as the green emitting layer in a class of organic light-emitting diodes. In this paper, the time dependence of the fluorescence from thin Alq3 films has been studied by means of the femtosecond fluorescence upconversion technique. From the temporally resolved

  18. Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins

    NARCIS (Netherlands)

    Blum, Christian; Meixner, Alfred J.; Subramaniam, Vinod

    2011-01-01

    DsRed is representative of the tetrameric reef coral fluorescent proteins that constitute particularly interesting coupled multichromophoric systems. Either a green emitting or a red emitting chromophore can form within each of the monomers of the protein tetramer. Within the tetramers the

  19. Bluish green emitting carbon quantum dots synthesized from jackfruit (Artocarpus heterophyllus) and its sensing applications of Hg (II) and Cr (VI) ions

    Science.gov (United States)

    Rajendran, Kalimuthu; Rajendiran, Nagappan

    2018-02-01

    A simple, economical, and green method for the preparation of water soluble, high fluorescent carbon quantum dots (CQDs) has been prepared via hydrothermal process using jackfruit (Artocarpus heterophyllus) as a carbon source. The optical properties of synthesized CQDs were characterized by UV- visible and fluorescence spectroscopy. Fourier transform infrared spectroscopy (FT-IR), x-ray Diffraction (XRD) and high resolution transmission electron microscopy (HR-TEM) techniques were used to study the composition and size of the CQDs. The prepared CQDs were spherical in shape with an average size of 2.5 nm along with uniform distribution and showed bright bluish green emission properties, without any further surface modification. The prepared CQDs were exhibit high stability at neutral pH and showed high photo-stability under UV light irradiation at 365 nm. The obtained CQDs were effectively utilized as fluorescent probe for highly selective and sensitive detection of Hg2+ and Cr6+ ions in environmental samples with a limit of detection of about 8 and 10 nM respectively.

  20. Photoluminescence and cathodoluminescence properties of green emitting SrGa2{S}4 : Eu2+ thin film

    Science.gov (United States)

    Chartier, Céline; Benalloul, Paul; Barthou, Charles; Frigerio, Jean-Marc; Mueller, Gerd O.; Mueller-Mach, Regina; Trottier, Troy

    2002-02-01

    Photoluminescence and cathodoluminescence properties of SrGa2S4 : Eu2+ thin films prepared by reactive RF magnetron sputtering are investigated. Luminescence performances of the phosphor in the thin film form are compared to those of powder samples: the brightness efficiency of thin films is found to be about 30% of the efficiency of powder at low current density. A ratio higher than 40% is expected at higher current density. Thin film screens for FEDs will become a positive alternative to powder screens provided that film quality and light extraction could be improved by optimization of thickness and deposition parameters.

  1. Rapid synthesis of spherical-shaped green-emitting MgGa2O4:Mn2+ phosphor via spray pyrolysis

    International Nuclear Information System (INIS)

    Choi, Sungho; Kim, Kyoungun; Moon, Young-Min; Park, Byung-Yoon; Jung, Ha-Kyun

    2010-01-01

    Simple, one-step synthesis of spherical-shaped powder phosphors with aqueous precursors via a spray pyrolysis method is reported. Green-emitting MgGa 2 O 4 :Mn 2+ phosphor with a controlled shape was successfully obtained by spraying under a reductive atmosphere (N 2 + H 2 carrier gas) without high-temperature post-heat treatment. In addition, the corresponding powder phosphors were well dispersed and showed a clean surface morphology compared to an existing cumbersome process using high-temperature post-annealing. The new method may help to prevent surface residual non-radiative defect sites. The result of highly luminescent and spherical morphology, non-aggregated powder phosphor by this procedure holds promise for a cost-effective and rapid synthesis process for conventional inorganic phosphors.

  2. Synthesis and luminescent properties of a novel green-emitting Tb (Ⅲ) complex based on amino-modified fluorine silicone oil and isophorone diisocyanate

    Science.gov (United States)

    Hao, Haixia; Chu, Yang; Yu, Zhenjiang; Xie, Hongde; Seo, Hyo Jin

    2017-10-01

    The novel luminescent polymer-rare earth complexes, denoted as (PFSi-IPDI)-Tb(Ⅲ)-Phen, have been successfully synthesized and can be made into flexible films. Amino-modified fluorine silicone oil-isophorone diisocyanate (PFSi-IPDI) was used as the host macromolecular ligand, and 1, 10-Phenanthroline (Phen) as the secondary small-molecular co-ligand. The luminescent lanthanide complexes were characterized by fourier transform infrared (FITR), scanning electron microscope (SEM), thermogravimetric analysis (TGA). The luminescent properties were investigated through photoluminescence excitation (PLE) and emission (PL) spectroscopy. FTIR analysis verifies the successful preparation and integration of PFSi-IPDI to Tb3+. The comparatively uniform morphological structure can be observed in the images of SEM. The polymer-rare earth complexes display the typical luminescence emission peaks under the excitation wavelength of 330 nm. From the decay curve, the short lifetime (about 0.89 ms) is observed for (PFSi-IPDI)-Tb(Ⅲ)-Phen (0.6 mol/L). Moreover, these luminescent polymer-rare earth complexes possess superior thermal stability (T5 > 195 °C). All the interesting results suggest the potential application of the luminescent polymer-rare earth complexes in green-emitting luminescent materials under high temperature.

  3. Long persistent luminescence property of a novel green emitting SrLaGaO{sub 4}: Tb{sup 3+} phosphor

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Xiaoyan, E-mail: fuxiaoyan@xmut.edu.cn [College of Materials Science and Engineering, Xiamen University of Technology, Xiamen 361024 (China); Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (CICAEET), Nanjing University of Information Science & Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Nanjing 210044 (China); Zheng, Shenghui [College of Materials Science and Engineering, Xiamen University of Technology, Xiamen 361024 (China); Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Shi, Junpeng [Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China); Li, Yuechan [College of Materials Science and Engineering, Xiamen University of Technology, Xiamen 361024 (China); Zhang, Hongwu, E-mail: hwzhang@iue.ac.cn [Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021 (China)

    2017-04-15

    A novel long persistent green emitting phosphor SrLaGaO{sub 4}: Tb{sup 3+} was synthesized via a conventional high temperature solid-state method. The obtained results indicated that the green long persistent emitting was similar to the photoluminescence, originating from the f-f transitions of Tb{sup 3+} centers which were supposed to occupy the random distribution Sr{sup 2+} and La{sup 3+} sites. The duration of green afterglow can be observed in the dark by naked eyes even after more than 3.5 h. The thermoluminescence results revealed that SrLaGaO{sub 4}: Tb{sup 3+} possessed three main traps calculated to be 0.62, 0.68 and 0.77 eV, which were responsible for the long persistent green luminescence. The further structure analysis revealed that the Tb{sup 3+} dopants not only acted as emission centers but also significantly influenced the density of traps, and the trapping centers were postulated nonrandom distribution under the assistance of high temperature, which resulted in the efficient persistent luminescence of Tb{sup 3+}. All the results showed that SrLaGaO{sub 4}: Tb{sup 3+} was a potential long persistent luminescent material.

  4. Preparation and luminescence of green-emitting ZnAl{sub 2}O{sub 4}:Mn{sup 2+} phosphor thin films

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Ing-Bang [Department of Materials Science and Engineering, National Formosa University, Huwei, Yunlin 632, Taiwan (China); Chang, Yee-Shin [Department of Electronic Engineering, National Formosa University, Huwei, Yunlin 632, Taiwan (China); Chen, Hao-Long [Department of Electronic Engineering, Kao Yuan University, Lujhu, Kaohsiung 821, Taiwan (China); Hwang, Ching Chiang [Department of Biotechnology, Mingdao University, Chang-Hua 52345, Taiwan (China); Jian, Chen-Jhu; Chen, Yu-Shiang [Department of Materials Science and Engineering, National Formosa University, Huwei, Yunlin 632, Taiwan (China); Tsai, Mu-Tsun, E-mail: mttsai@ms23.hinet.net [Department of Materials Science and Engineering, National Formosa University, Huwei, Yunlin 632, Taiwan (China)

    2014-11-03

    Nanocrystalline Mn{sup 2+}-doped zinc spinel (ZnAl{sub 2}O{sub 4}:Mn{sup 2+}) green-emitting phosphor films were deposited on silicon substrate by sol–gel spin coating and subsequent heat treatment up to 1000 °C. The effects of dopant concentration and heat treatment on the optical and structural properties were investigated. The variations in sol viscosity with time, film thickness with number of layers were also examined. Thin films were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray microscopy, atomic force microscopy, and photoluminescence spectrum. Single-phase ZnAl{sub 2}O{sub 4} started to crystallize at around 600 °C, with a normal spinel structure. On annealing at 1000 °C, the films had smooth surfaces with a nanocrystalline structure. Under UV or visible light excitation, the phosphor films exhibited an intense green emission band peaking at around 512 nm, corresponding to the typical {sup 4}T{sub 1} → {sup 6}A{sub 1} transition of tetrahedral Mn{sup 2+} ions. The most intense green emission was obtained by exciting at 456 nm. The emission intensity of films was highly dependent upon the excitation wavelength, crystallinity, dopant content, and deposition conditions. The results show that the ZnAl{sub 2}O{sub 4}:Mn{sup 2+} films have good potential for use as a green phosphor for displays and/or white light-emitting diodes. - Highlights: • ZnAl2O4:Mn2 + thin film phosphors have been synthesized by a sol–gel process. • The most intense green emission was obtained by exciting at 456 nm. • Photoluminescence is highly dependent on the crystallinity and doping content. • Emission intensity can also be modulated by controlling the film thickness.

  5. Controlled light emission from white organic light-emitting devices with a single blue-emitting host and multiple fluorescent dopants

    International Nuclear Information System (INIS)

    Chin, Byung Doo; Kim, Jai Kyeong; Park, O Ok

    2007-01-01

    In this work, we fabricated white organic light-emitting devices (WOLEDs) containing a layered light-emitting region composed of a single blue-emitting host and different fluorescent dopant materials. The effects of varying the dye-doping ratio and emitting layer thickness on the efficiency, lifetime, spectral voltage-dependence and white balance were investigated for devices with a blue/orange stacked layer structure. Addition of a blue host layer doped with a green-emitting dopant, to give a blue/green/orange emitter, resulted in a broadband white spectrum without the need for a charge-blocking interlayer. The composition of blue, green and orange dopants in the host and the thickness of each emitting layer were optimized, resulting in a device efficiency of 9-11 cd A -1 even at a high brightness of 10 000 cd m -2 (achieved at a bias voltage of less than 9 V) with an emission spectrum suitable for lighting applications

  6. Burkina Faso - BRIGHT II

    Data.gov (United States)

    Millennium Challenge Corporation — Millennium Challenge Corporation hired Mathematica Policy Research to conduct an independent evaluation of the BRIGHT II program. The three main research questions...

  7. Highly Efficient Green-Emitting Phosphors Ba2Y5B5O17 with Low Thermal Quenching Due to Fast Energy Transfer from Ce3+ to Tb3.

    Science.gov (United States)

    Xiao, Yu; Hao, Zhendong; Zhang, Liangliang; Xiao, Wenge; Wu, Dan; Zhang, Xia; Pan, Guo-Hui; Luo, Yongshi; Zhang, Jiahua

    2017-04-17

    This paper demonstrates a highly thermally stable and efficient green-emitting Ba 2 Y 5 B 5 O 17 :Ce 3+ , Tb 3+ phosphor prepared by high-temperature solid-state reaction. The phosphor exhibits a blue emission band of Ce 3+ and green emission lines of Tb 3+ upon Ce 3+ excitation in the near-UV spectral region. The effect of Ce 3+ to Tb 3+ energy transfer on blue to green emission color tuning and on luminescence thermal stability is studied in the samples codoped with 1% Ce 3+ and various concentrations (0-40%) of Tb 3+ . The green emission of Tb 3+ upon Ce 3+ excitation at 150 °C can keep, on average, 92% of its intensity at room temperature, with the best one showing no intensity decreasing up to 210 °C for 30% Tb 3+ . Meanwhile, Ce 3+ emission intensity only keeps 42% on average at 150 °C. The high thermal stability of the green emission is attributed to suppression of Ce 3+ thermal de-excitation through fast energy transfer to Tb 3+ , which in the green-emitting excited states is highly thermally stable such that no lifetime shortening is observed with raising temperature to 210 °C. The predominant green emission is observed for Tb 3+ concentration of at least 10% due to efficient energy transfer with the transfer efficiency approaching 100% for 40% Tb 3+ . The internal and external quantum yield of the sample with Tb 3+ concentration of 20% can be as high as 76% and 55%, respectively. The green phosphor, thus, shows attractive performance for near-UV-based white-light-emitting diodes applications.

  8. BrightFocus Foundation

    Science.gov (United States)

    ... About BrightFocus Foundation Featured Content BrightFocus: Investing in Science to Save Mind and Sight We're here to help. Explore ... recognition is very important. Monday, November 6, 2017 New Diagnosis? Managing a mind and sight disease is a journey. And you’ ...

  9. N-2-Aryl-1,2,3-Triazoles: A novel class of blue–green emitting fluorophores-synthesis, photophysical properties study and dft computations

    Energy Technology Data Exchange (ETDEWEB)

    Padalkar, Vikas S.; Chemate, Santosh B.; Lanke, Sandip K.; Sekar, Nagaiyan, E-mail: n.sekar@ictmumbai.edu.in

    2015-12-15

    Three novel 2-{2-[4-(N,N-diethylamino)–2–hydroxyphenyl]–1H–benzo[d]imidazol–6–yl} –2H–naphtho [1,2-d] [1,2,3] fluorescent triazole derivatives were synthesized from 2-(5-amino-1H-benzimidazol-2-yl)-5-(N,N-diethylamino)phenol and amino substituted naphthylsulphonic acids. The absorption, emission, quantum yields and dipole moments of these compounds were evaluated in methanol, acetonitrile, N,N-dimethylformamide and dimethylsulfoxide. The compounds 8a and 8c absorb in the near visible region, while compound 8b shows two absorption peaks, short wavelength peak is in the near visible region and long wavelength absorption in the visible region. Compounds are fluorescent in solution and emit in blue and green region. The photophysical properties of the 8a–8c were compared with structural analogs reported till date. The experimental absorption and emission properties were compared with the theoretical data obtained by DFT and TD-DFT computations with TD–B3LYP and CAM–B3LYP functional with 6–31G (d) and 6–311G (d) basis sets. Theoretical results obtained by TD-B3LYP functional are well in line with the experimental results. The compounds are thermally stable up to 300 °C. New compounds were characterized by spectral techniques. - Highlights: • First unique study of blue-emitting ESIPT triazoles. • Improved photophysical properties compared to similar analogues. • Experimental and TDDFT data on photophysical properties. • Dipole moments from solvatochromic data.

  10. Stunningly bright optical emission

    Science.gov (United States)

    Heinke, Craig O.

    2017-12-01

    The detection of bright, rapid optical pulsations from pulsar PSR J1023+0038 have provided a surprise for researchers working on neutron stars. This discovery poses more questions than it answers and will spur on future work and instrumentation.

  11. One-step solvothermal synthesis, a worm-shaped morphology and luminescence properties of green-emitting Y2O2S:Tb3+ nanophosphors

    Science.gov (United States)

    Liu, Fan; Lian, Jingbao; Wu, Nianchu; He, Jiao; Zhang, Xue; Liu, Feng

    2018-02-01

    The worm-shaped Y2O2S:Tb3+ nanophosphors have been successfully prepared via one-step solvothermal synthesis, using ethanediamine as the main solvent and sublimed sulfur powder as the source of sulfur without adding additional structure-directing reagents. XRD, FT-IR, FESEM, PL and CIE chromaticity diagram were used to characterize the obtained products. XRD results demonstrate that all diffraction peaks of the sample can be well indexed to pure hexagonal phase of Y2O2S with optimal condition, i.e. the molar ration of S/Y3+m = 10, solvothermal temperature T = 220 °C and synthetic time t = 24 h. When t varies from 2 h to 24 h, the morphology of Y2O2S transforms from cauliflower-like structure to worm-shaped nanoparticles with the length of ∼80 nm. The formation mechanism depending on t has also been proposed. Upon 250 nm ultraviolet (UV) light excitation, the worm-shaped Y2O2S:Tb3+ nanophosphors exhibit green emissions, corresponding to the 5D4 → 7FJ (J = 6, 5, 4, 3) transitions of Tb3+ions. The quenching concentration is 7% and its corresponding lifetime is 1216 μs. The CIE chromaticity coordinates show the tuneable emission shifting from yellow green to yellowish green with increasing concentration of Tb3+ions from 1% to 7%. Those results suggest that the worm-shaped Y2O2S:Tb3+ nanophosphors may have potential applications in X-ray intensifying screens, fluorescence and biomedical fields.

  12. High Brightness OLED Lighting

    Energy Technology Data Exchange (ETDEWEB)

    Spindler, Jeffrey [OLEDWorks LLC; Kondakova, Marina [OLEDWorks LLC; Boroson, Michael [OLEDWorks LLC; Hamer, John [OLEDWorks LLC

    2016-05-25

    In this work we describe the technology developments behind our current and future generations of high brightness OLED lighting panels. We have developed white and amber OLEDs with excellent performance based on the stacking approach. Current products achieve 40-60 lm/W, while future developments focus on achieving 80 lm/W or higher.

  13. High brightness ion source

    International Nuclear Information System (INIS)

    Dreyfus, R.W.; Hodgson, R.T.

    1975-01-01

    A high brightness ion beam is obtainable by using lasers to excite atoms or molecules from the ground state to an ionized state in increments, rather than in one step. The spectroscopic resonances of the atom or molecule are used so that relatively long wavelength, low power lasers can be used to obtain such ion beam

  14. The brightness of colour.

    Directory of Open Access Journals (Sweden)

    David Corney

    Full Text Available The perception of brightness depends on spatial context: the same stimulus can appear light or dark depending on what surrounds it. A less well-known but equally important contextual phenomenon is that the colour of a stimulus can also alter its brightness. Specifically, stimuli that are more saturated (i.e. purer in colour appear brighter than stimuli that are less saturated at the same luminance. Similarly, stimuli that are red or blue appear brighter than equiluminant yellow and green stimuli. This non-linear relationship between stimulus intensity and brightness, called the Helmholtz-Kohlrausch (HK effect, was first described in the nineteenth century but has never been explained. Here, we take advantage of the relative simplicity of this 'illusion' to explain it and contextual effects more generally, by using a simple Bayesian ideal observer model of the human visual ecology. We also use fMRI brain scans to identify the neural correlates of brightness without changing the spatial context of the stimulus, which has complicated the interpretation of related fMRI studies.Rather than modelling human vision directly, we use a Bayesian ideal observer to model human visual ecology. We show that the HK effect is a result of encoding the non-linear statistical relationship between retinal images and natural scenes that would have been experienced by the human visual system in the past. We further show that the complexity of this relationship is due to the response functions of the cone photoreceptors, which themselves are thought to represent an efficient solution to encoding the statistics of images. Finally, we show that the locus of the response to the relationship between images and scenes lies in the primary visual cortex (V1, if not earlier in the visual system, since the brightness of colours (as opposed to their luminance accords with activity in V1 as measured with fMRI.The data suggest that perceptions of brightness represent a robust

  15. The Bright Universe Cosmology

    International Nuclear Information System (INIS)

    Surdin, M.

    1980-01-01

    It is shown that viewed from the 'outside', our universe is a black hole. Hence the 'inside' cosmology considered is termed as the Bright Universe Cosmology. The model proposed avoids the singularities of cosmologies of the Big Bang variety, it gives a good account of the redshifts, the cosmic background radiation, the number counts; it also gives a satisfactory explanation of the 'large numbers coincidence' and of the variation in time of fundamental constants. (Auth.)

  16. Kiloamp high-brightness beams

    International Nuclear Information System (INIS)

    Caporaso, G.J.

    1987-01-01

    Brightness preservation of high-current relativistic electron beams under two different types of transport is discussed. Recent progress in improving the brightness of laser-guided beams in the Advanced Test Accelerator is reviewed. A strategy for the preservation of the brightness of space-charge-dominated beams in a solenoidal transport system is presented

  17. A high brightness probe of polymer nanoparticles for biological imaging

    Science.gov (United States)

    Zhou, Sirong; Zhu, Jiarong; Li, Yaping; Feng, Liheng

    2018-03-01

    Conjugated polymer nanoparticles (CPNs) with high brightness in long wavelength region were prepared by the nano-precipitation method. Based on fluorescence resonance energy transfer (FRET) mechanism, the high brightness property of the CPNs was realized by four different emission polymers. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) displayed that the CPNs possessed a spherical structure and an average diameter of 75 nm. Analysis assays showed that the CPNs had excellent biocompatibility, good photostability and low cytotoxicity. The CPNs were bio-modified with a cell penetrating peptide (Tat, a targeted element) through covalent link. Based on the entire wave fluorescence emission, the functionalized CPNs1-4 can meet multichannel and high throughput assays in cell and organ imaging. The contribution of the work lies in not only providing a new way to obtain a high brightness imaging probe in long wavelength region, but also using targeted cell and organ imaging.

  18. Bright point study

    International Nuclear Information System (INIS)

    Tang, F.; Harvey, K.; Bruner, M.; Kent, B.; Antonucci, E.

    1982-01-01

    Transition region and coronal observations of bright points by instruments aboard the Solar Maximum Mission and high resolution photospheric magnetograph observations on September 11, 1980 are presented. A total of 31 bipolar ephemeral regions were found in the photosphere from birth in 9.3 hours of combined magnetograph observations from three observatories. Two of the three ephemeral regions present in the field of view of the Ultraviolet Spectrometer-Polarimeter were observed in the C IV 1548 line. The unobserved ephemeral region was determined to be the shortest-lived (2.5 hr) and lowest in magnetic flux density (13G) of the three regions. The Flat Crystal Spectrometer observed only low level signals in the O VIII 18.969 A line, which were not statistically significant to be positively identified with any of the 16 ephemeral regions detected in the photosphere. In addition, the data indicate that at any given time there lacked a one-to-one correspondence between observable bright points and photospheric ephemeral regions, while more ephemeral regions were observed than their counterparts in the transition region and the corona

  19. Combined "dual" absorption and fluorescence smartphone spectrometers.

    Science.gov (United States)

    Arafat Hossain, Md; Canning, John; Ast, Sandra; Cook, Kevin; Rutledge, Peter J; Jamalipour, Abbas

    2015-04-15

    A combined "dual" absorption and fluorescence smartphone spectrometer is demonstrated. The optical sources used in the system are the white flash LED of the smartphone and an orthogonally positioned and interchangeable UV (λex=370  nm) and blue (λex=450  nm) LED. The dispersive element is a low-cost, nano-imprinted diffraction grating coated with Au. Detection over a 300 nm span with 0.42 nm/pixel resolution was carried out with the camera CMOS chip. By integrating the blue and UV excitation sources into the white LED circuitry, the entire system is self-contained within a 3D printed case and powered from the smartphone battery; the design can be scaled to add further excitation sources. Using a customized app, acquisition of absorption and fluorescence spectra are demonstrated using a blue-absorbing and green-emitting pH-sensitive amino-naphthalimide-based fluorescent probe and a UV-absorbing and blue-emitting Zn2+-sensitive fluoro-ionophore.

  20. Quantitative measurement of brightness from living cells in the presence of photodepletion.

    Directory of Open Access Journals (Sweden)

    Kwang-Ho Hur

    Full Text Available The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Here, we focus on the cumulative influence of photobleaching on brightness measurements in cells. Photobleaching within the finite volume of the cell leads to a depletion of the population of fluorescently labeled proteins with time. The process of photodepletion reduces the fluorescence signal which biases the analysis of brightness data. Our data show that even small reductions in the signal can introduce significant bias into the analysis of the data. We develop a model that quantifies the bias and introduce an analysis method that accurately determines brightness in the presence of photodepletion as verified by experiments with mammalian and yeast cells. In addition, photodepletion experiments with the fluorescent protein EGFP reveal the presence of a photoconversion process, which leads to a marked decrease in the brightness of the EGFP protein. We also identify conditions where the effect of EGFP's photoconversion on brightness experiments can be safely ignored.

  1. High brightness electron accelerator

    International Nuclear Information System (INIS)

    Sheffield, R.L.; Carlsten, B.E.; Young, L.M.

    1994-01-01

    A compact high brightness linear accelerator is provided for use, e.g., in a free electron laser. The accelerator has a first plurality of accelerating cavities having end walls with four coupling slots for accelerating electrons to high velocities in the absence of quadrupole fields. A second plurality of cavities receives the high velocity electrons for further acceleration, where each of the second cavities has end walls with two coupling slots for acceleration in the absence of dipole fields. The accelerator also includes a first cavity with an extended length to provide for phase matching the electron beam along the accelerating cavities. A solenoid is provided about the photocathode that emits the electrons, where the solenoid is configured to provide a substantially uniform magnetic field over the photocathode surface to minimize emittance of the electrons as the electrons enter the first cavity. 5 figs

  2. Thermally stable green Ba(3)Y(PO(4))3:Ce(3+),Tb(3+) and red Ca(3)Y(AlO)(3)(BO(3))4:Eu(3+) phosphors for white-light fluorescent lamps.

    Science.gov (United States)

    Huang, Chien-Hao; Kuo, Te-Wen; Chen, Teng-Ming

    2011-01-03

    A class of thermal stable of green-emitting phosphors Ba(3)Y(PO(4))(3):Ce(3+),Tb(3+) (BYP:Ce(3+),Tb(3+)) and red-emitting phosphors Ca(3)Y(AlO)(3)(BO(3))(4):Eu(3+) (CYAB:Eu(3+)) for white-light fluorescent lamps were synthesized by high temperature solid-state reaction. We observed a decay of only 3% at 150 °C for BYP:0.25Ce3+,0.25Tb3+ (3% for LaPO4:Ce(3+),Tb(3+)), and a decay of 4% for CYAB:0.5Eu(3+) (7% for Y(2)O(3):Eu(3+), 24% for Y(2)O(2)S:Eu(3+)). The emission intensity of composition-optimized Ba(3)(Y(0.5)Ce(0.25)Tb(0.25))(PO(4))(3) is 70% of that of commercial LaPO(4):Ce(3+),Tb(3+) phosphors, and the CIE chromaticity coordinates are found to be (0.323, 0.534). The emission intensity of Ca(3)(Y(0.5)Eu(0.5))(AlO)(3)(BO(3))(4) is 70% and 83% of those of Y(2)O(3):Eu(3+) and Y(2)O(2)S:Eu(3+) phosphors, respectively, and the CIE chromaticity coordinates are redder (0.652, 0.342) than those of Y(2)O(3):Eu(3+) (0.645, 0.347) and Y(2)O(2)S:Eu(3+) (0.647, 0.343). A white-light fluorescent lamp is fabricated using composition-optimized Ba(3)(Y(0.5)Ce(0.25)Tb(0.25))(PO(4))(3) and Ca(3)(Y(0.5)Eu(0.5))(AlO)(3)(BO(3))(4) phosphors and matching blue-emitting phosphors. The results indicate that the quality of the brightness and color reproduction is suitable for application in shortwave UV fluorescent lamps. The white-light fluorescent lamp displays CIE chromaticity coordinates of x = 0.33, y = 0.35, a warm white light with a correlated color temperature of 5646 K, and a color-rendering index of Ra = 70.

  3. The ZTF Bright Transient Survey

    Science.gov (United States)

    Fremling, C.; Sharma, Y.; Kulkarni, S. R.; Miller, A. A.; Taggart, K.; Perley, D. A.; Gooba, A.

    2018-06-01

    As a supplement to the Zwicky Transient Facility (ZTF; ATel #11266) public alerts (ATel #11685) we plan to report (following ATel #11615) bright probable supernovae identified in the raw alert stream from the ZTF Northern Sky Survey ("Celestial Cinematography"; see Bellm & Kulkarni, 2017, Nature Astronomy 1, 71) to the Transient Name Server (https://wis-tns.weizmann.ac.il) on a daily basis; the ZTF Bright Transient Survey (BTS; see Kulkarni et al., 2018; arXiv:1710.04223).

  4. Control of excitation in the fluorescence microscope.

    Science.gov (United States)

    Lea, D J; Ward, D J

    1979-01-01

    In fluorescence microscopy image brightness and contrast and the rate of fading depend upon the intensity of illumination of the specimen. An iris diaphragm or neutral density filters may be used to reduce fluorescence excitation. Also the excitation bandwidth may be varied by using a broad band exciter filter with a set of interchangeable yellow glass filters at the lamphouse.

  5. Teradiode's high brightness semiconductor lasers

    Science.gov (United States)

    Huang, Robin K.; Chann, Bien; Burgess, James; Lochman, Bryan; Zhou, Wang; Cruz, Mike; Cook, Rob; Dugmore, Dan; Shattuck, Jeff; Tayebati, Parviz

    2016-03-01

    TeraDiode is manufacturing multi-kW-class ultra-high brightness fiber-coupled direct diode lasers for industrial applications. A fiber-coupled direct diode laser with a power level of 4,680 W from a 100 μm core diameter, BPP) of 3.5 mm-mrad and is the lowest BPP multi-kW-class direct diode laser yet reported. This laser is suitable for industrial materials processing applications, including sheet metal cutting and welding. This 4-kW fiber-coupled direct diode laser has comparable brightness to that of industrial fiber lasers and CO2 lasers, and is over 10x brighter than state-of-the-art direct diode lasers. We have also demonstrated novel high peak power lasers and high brightness Mid-Infrared Lasers.

  6. Fluorescence fluctuation spectroscopy (FFS), part A

    CERN Document Server

    Tetin, Sergey

    2013-01-01

    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers fluorescence fluctuation spectroscopyContains chapte

  7. A spectral k-means approach to bright-field cell image segmentation.

    Science.gov (United States)

    Bradbury, Laura; Wan, Justin W L

    2010-01-01

    Automatic segmentation of bright-field cell images is important to cell biologists, but difficult to complete due to the complex nature of the cells in bright-field images (poor contrast, broken halo, missing boundaries). Standard approaches such as level set segmentation and active contours work well for fluorescent images where cells appear as round shape, but become less effective when optical artifacts such as halo exist in bright-field images. In this paper, we present a robust segmentation method which combines the spectral and k-means clustering techniques to locate cells in bright-field images. This approach models an image as a matrix graph and segment different regions of the image by computing the appropriate eigenvectors of the matrix graph and using the k-means algorithm. We illustrate the effectiveness of the method by segmentation results of C2C12 (muscle) cells in bright-field images.

  8. Helmholtz bright and boundary solitons

    Energy Technology Data Exchange (ETDEWEB)

    Christian, J M [Joule Physics Laboratory, School of Computing, Science and Engineering, Institute for Materials Research, University of Salford, Salford M5 4WT (United Kingdom); McDonald, G S [Joule Physics Laboratory, School of Computing, Science and Engineering, Institute for Materials Research, University of Salford, Salford M5 4WT (United Kingdom); Chamorro-Posada, P [Departmento de TeorIa de la Senal y Comunicaciones e IngenierIa Telematica, Universidad de Valladolid, ETSI Telecomunicacion, Campus Miguel Delibes s/n, 47011 Valladolid (Spain)

    2007-02-16

    We report, for the first time, exact analytical boundary solitons of a generalized cubic-quintic nonlinear Helmholtz (NLH) equation. These solutions have a linked-plateau topology that is distinct from conventional dark soliton solutions; their amplitude and intensity distributions are spatially delocalized and connect regions of finite and zero wave-field disturbances (suggesting also the classification as 'edge solitons'). Extensive numerical simulations compare the stability properties of recently derived Helmholtz bright solitons, for this type of polynomial nonlinearity, to those of the new boundary solitons. The latter are found to possess a remarkable stability characteristic, exhibiting robustness against perturbations that would otherwise lead to the destabilizing of their bright-soliton counterparts.

  9. A New Sky Brightness Monitor

    Science.gov (United States)

    Crawford, David L.; McKenna, D.

    2006-12-01

    A good estimate of sky brightness and its variations throughout the night, the months, and even the years is an essential bit of knowledge both for good observing and especially as a tool in efforts to minimize sky brightness through local action. Hence a stable and accurate monitor can be a valuable and necessary tool. We have developed such a monitor, with the financial help of Vatican Observatory and Walker Management. The device is now undergoing its Beta test in preparation for production. It is simple, accurate, well calibrated, and automatic, sending its data directly to IDA over the internet via E-mail . Approximately 50 such monitors will be ready soon for deployment worldwide including most major observatories. Those interested in having one should enquire of IDA about details.

  10. High-brightness injector modeling

    International Nuclear Information System (INIS)

    Lewellen, J.W.

    2004-01-01

    There are many aspects to the successful conception, design, fabrication, and operation of high-brightness electron beam sources. Accurate and efficient modeling of the injector are critical to all phases of the process, from evaluating initial ideas to successful diagnosis of problems during routine operation. The basic modeling tasks will vary from design to design, according to the basic nature of the injector (dc, rf, hybrid, etc.), the type of cathode used (thermionic, photo, field emitter, etc.), and 'macro' factors such as average beam current and duty factor, as well as the usual list of desired beam properties. The injector designer must be at least aware of, if not proficient at addressing, the multitude of issues that arise from these considerations; and, as high-brightness injectors continue to move out of the laboratory, the number of such issues will continue to expand.

  11. Helmholtz bright and boundary solitons

    International Nuclear Information System (INIS)

    Christian, J M; McDonald, G S; Chamorro-Posada, P

    2007-01-01

    We report, for the first time, exact analytical boundary solitons of a generalized cubic-quintic nonlinear Helmholtz (NLH) equation. These solutions have a linked-plateau topology that is distinct from conventional dark soliton solutions; their amplitude and intensity distributions are spatially delocalized and connect regions of finite and zero wave-field disturbances (suggesting also the classification as 'edge solitons'). Extensive numerical simulations compare the stability properties of recently derived Helmholtz bright solitons, for this type of polynomial nonlinearity, to those of the new boundary solitons. The latter are found to possess a remarkable stability characteristic, exhibiting robustness against perturbations that would otherwise lead to the destabilizing of their bright-soliton counterparts

  12. Giant Low Surface Brightness Galaxies

    Science.gov (United States)

    Mishra, Alka; Kantharia, Nimisha G.; Das, Mousumi

    2018-04-01

    In this paper, we present radio observations of the giant low surface brightness (LSB) galaxies made using the Giant Metrewave Radio Telescope (GMRT). LSB galaxies are generally large, dark matter dominated spirals that have low star formation efficiencies and large HI gas disks. Their properties suggest that they are less evolved compared to high surface brightness galaxies. We present GMRT emission maps of LSB galaxies with an optically-identified active nucleus. Using our radio data and archival near-infrared (2MASS) and near-ultraviolet (GALEX) data, we studied morphology and star formation efficiencies in these galaxies. All the galaxies show radio continuum emission mostly associated with the centre of the galaxy.

  13. High brightness semiconductor lasers with reduced filamentation

    DEFF Research Database (Denmark)

    McInerney, John; O'Brien, Peter.; Skovgaard, Peter M. W.

    1999-01-01

    High brightness semiconductor lasers have applications in spectroscopy, fiber lasers, manufacturing and materials processing, medicine and free space communication or energy transfer. The main difficulty associated with high brightness is that, because of COD, high power requires a large aperture...

  14. Quantitative Image Restoration in Bright Field Optical Microscopy.

    Science.gov (United States)

    Gutiérrez-Medina, Braulio; Sánchez Miranda, Manuel de Jesús

    2017-11-07

    Bright field (BF) optical microscopy is regarded as a poor method to observe unstained biological samples due to intrinsic low image contrast. We introduce quantitative image restoration in bright field (QRBF), a digital image processing method that restores out-of-focus BF images of unstained cells. Our procedure is based on deconvolution, using a point spread function modeled from theory. By comparing with reference images of bacteria observed in fluorescence, we show that QRBF faithfully recovers shape and enables quantify size of individual cells, even from a single input image. We applied QRBF in a high-throughput image cytometer to assess shape changes in Escherichia coli during hyperosmotic shock, finding size heterogeneity. We demonstrate that QRBF is also applicable to eukaryotic cells (yeast). Altogether, digital restoration emerges as a straightforward alternative to methods designed to generate contrast in BF imaging for quantitative analysis. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  15. Bright triplet excitons in caesium lead halide perovskites

    Science.gov (United States)

    Becker, Michael A.; Vaxenburg, Roman; Nedelcu, Georgian; Sercel, Peter C.; Shabaev, Andrew; Mehl, Michael J.; Michopoulos, John G.; Lambrakos, Samuel G.; Bernstein, Noam; Lyons, John L.; Stöferle, Thilo; Mahrt, Rainer F.; Kovalenko, Maksym V.; Norris, David J.; Rainò, Gabriele; Efros, Alexander L.

    2018-01-01

    Nanostructured semiconductors emit light from electronic states known as excitons. For organic materials, Hund’s rules state that the lowest-energy exciton is a poorly emitting triplet state. For inorganic semiconductors, similar rules predict an analogue of this triplet state known as the ‘dark exciton’. Because dark excitons release photons slowly, hindering emission from inorganic nanostructures, materials that disobey these rules have been sought. However, despite considerable experimental and theoretical efforts, no inorganic semiconductors have been identified in which the lowest exciton is bright. Here we show that the lowest exciton in caesium lead halide perovskites (CsPbX3, with X = Cl, Br or I) involves a highly emissive triplet state. We first use an effective-mass model and group theory to demonstrate the possibility of such a state existing, which can occur when the strong spin-orbit coupling in the conduction band of a perovskite is combined with the Rashba effect. We then apply our model to CsPbX3 nanocrystals, and measure size- and composition-dependent fluorescence at the single-nanocrystal level. The bright triplet character of the lowest exciton explains the anomalous photon-emission rates of these materials, which emit about 20 and 1,000 times faster than any other semiconductor nanocrystal at room and cryogenic temperatures, respectively. The existence of this bright triplet exciton is further confirmed by analysis of the fine structure in low-temperature fluorescence spectra. For semiconductor nanocrystals, which are already used in lighting, lasers and displays, these excitons could lead to materials with brighter emission. More generally, our results provide criteria for identifying other semiconductors that exhibit bright excitons, with potential implications for optoelectronic devices.

  16. Electron beam brightness with field immersed emission

    International Nuclear Information System (INIS)

    Boyd, J.K.; Neil, V.K.

    1985-01-01

    The beam quality or brightness of an electron beam produced with field immersed emission is studied with two models. First, an envelope formulation is used to determine the scaling of brightness with current, magnetic field and cathode radius, and examine the equilibrium beam radius. Second, the DPC computer code is used to calculate the brightness of two electron beam sources

  17. Does low surface brightness mean low density?

    NARCIS (Netherlands)

    deBlok, WJG; McGaugh, SS

    1996-01-01

    We compare the dynamical properties of two galaxies at identical positions on the Tully-Fisher relation, but with different surface brightnesses. We find that the low surface brightness galaxy UGC 128 has a higher mass-to-light ratio, and yet has lower mass densities than the high surface brightness

  18. High-brightness rf linear accelerators

    International Nuclear Information System (INIS)

    Jameson, R.A.

    1986-01-01

    The issue of high brightness and its ramifications in linacs driven by radio-frequency fields is discussed. A history of the RF linacs is reviewed briefly. Some current applications are then examined that are driving progress in RF linacs. The physics affecting the brightness of RF linacs is then discussed, followed by the economic feasibility of higher brightness machines

  19. BODIPY-based fluorescent liposomes with sesquiterpene lactone trilobolide

    Directory of Open Access Journals (Sweden)

    Ludmila Škorpilová

    2017-07-01

    Full Text Available Like thapsigargin, which is undergoing clinical trials, trilobolide is a natural product with promising anticancer and anti-inflammatory properties. Similar to thapsigargin, it has limited aqueous solubility that strongly reduces its potential medicinal applications. The targeted delivery of hydrophobic drugs can be achieved using liposome-based carriers. Therefore, we designed a traceable liposomal drug delivery system for trilobolide. The fluorescent green-emitting dye BODIPY, cholesterol and trilobolide were used to create construct 6. The liposomes were composed of dipalmitoyl-3-trimethylammoniumpropane and phosphatidylethanolamine. The whole system was characterized by atomic force microscopy, the average size of the liposomes was 150 nm in width and 30 nm in height. We evaluated the biological activity of construct 6 and its liposomal formulation, both of which showed immunomodulatory properties in primary rat macrophages. The uptake and intracellular distribution of construct 6 and its liposomal formulation was monitored by means of live-cell fluorescence microscopy in two cancer cell lines. The encapsulation of construct 6 into the liposomes improved the drug distribution in cancer cells and was followed by cell death. This new liposomal trilobolide derivative not only retains the biological properties of pure trilobolide, but also enhances the bioavailability, and thus has potential for the use in theranostic applications.

  20. Development of Tb{sup 3+} activated gadolinium aluminate garnet (Gd{sub 3}Al{sub 5}O{sub 12}) as highly efficient green-emitting phosphors

    Energy Technology Data Exchange (ETDEWEB)

    Teng, Xin; Li, Jinkai, E-mail: mse_lijk@ujn.edu.cn; Duan, Guangbin; Liu, Zongming, E-mail: ost_liuzm@ujn.edu.cn

    2016-11-15

    The powder processing of (Gd{sub 1-x}Tb{sub x}){sub 3}Al{sub 5}O{sub 12} (GdAG:Tb{sup 3+}) solid solutions was achieved through precursor synthesized via carbonate precipitation, followed by calcination at 1500 °C. The performance of the GdAG:Tb{sup 3+} phosphors were characterized by the combined techniques of XRD, FE-SEM, HR-TEM, PLE/PL, and fluorescence decay analyses. Lower Tb{sup 3+} doping can stabilize the crystal structure of GdAG garnet against its thermal decomposition. The GdAG:Tb{sup 3+} phosphors with good dispersion and fairly uniform particle morphology exhibit a series of {sup 5}D{sub 4}–{sup 7}F{sub J} transitions of Tb{sup 3+} with strongest green emission at ~544 nm ({sup 5}D{sub 4}–{sup 7}F{sub 5} transitions of Tb{sup 3+}) under UV excitation at ~277 nm (4f{sup 8}−4f{sup 7}5d{sup 1} transition of Tb{sup 3+}). The later is overlapping with {sup 8}S{sub 7/2}–{sup 6}I{sub J} intra f–f transition of Gd{sup 3+} indirectly suggesting the existence of energy transfer from Gd{sup 3+} to Tb{sup 3+}. With the Gd{sup 3+}–Tb{sup 3+} energy transfer, higher Tb{sup 3+} emission and quantum efficiency than the well-known YAG:Tb{sup 3+} and LuAG:Tb{sup 3+} were obtained in the present work. The effects of Tb{sup 3+} content on luminescent property of the phosphor, especially PLE/PL properties, fluorescence lifetime and quantum efficient, were thoroughly investigated, which were also compared to those of Tb{sup 3+}-activated YAG and LuAG compounds. The CIE chromaticity coordinates and quenching concentration of GdAG:Tb{sup 3+} were determined to be (~0.37, ~0.56) and ~10 at%, respectively. Keeping the optimum Tb{sup 3+} content at ~10 at%, the (Gd{sub 0.9}Tb{sub 0.1})AG phosphor possesses high internal and external quantum efficiencies of ~88.7% and ~73.6% under ~277 nm excitation, respectively. Owing to its improved luminescent property and high theoretical density, the phosphors of (Gd{sub 1-x}Tb{sub x})AG garnet developed in the present work are

  1. Low surface brightness spiral galaxies

    International Nuclear Information System (INIS)

    Romanishin, W.

    1980-01-01

    This dissertation presents an observational overview of a sample of low surface brightness (LSB) spiral galaxies. The sample galaxies were chosen to have low surface brightness disks and indications of spiral structure visible on the Palomar Sky Survey. They are of sufficient angular size (diameter > 2.5 arcmin), to allow detailed surface photometry using Mayall 4-m prime focus plates. The major findings of this dissertation are: (1) The average disk central surface brightness of the LSB galaxies is 22.88 magnitude/arcsec 2 in the B passband. (2) From broadband color measurements of the old stellar population, we infer a low average stellar metallicity, on the order of 1/5 solar. (3) The spectra and optical colors of the HII regions in the LSB galaxies indicate a lack of hot ionizing stars compared to HII regions in other late-type galaxies. (4) The average surface mass density, measured within the radius containing half the total mass, is less than half that of a sample of normal late-type spirals. (5) The average LSB galaxy neutral hydrogen mass to blue luminosity ratio is about 0.6, significantly higher than in a sample of normal late-type galaxies. (6) We find no conclusive evidence of an abnormal mass-to-light ratio in the LSB galaxies. (7) Some of the LSB galaxies exhibit well-developed density wave patterns. (8) A very crude calculation shows the lower metallicity of the LSB galaxies compared with normal late-type spirals might be explained simply by the deficiency of massive stars in the LSB galaxies

  2. High brightness beams and applications

    International Nuclear Information System (INIS)

    Sheffield, R.L.

    1995-01-01

    This paper describes the present research on attaining intense bright electron beams. Thermionic systems are briefly covered. Recent and past results from the photoinjector programs are given. The performance advantages and difficulties presently faced by researchers using photoinjectors is discussed. The progress that has been made in photocathode materials, both in lifetime and quantum efficiency, is covered. Finally, a discussion of emittance measurements of photoinjector systems and how the measurement is complicated by the non-thermal nature of the electron beam is presented

  3. High-brightness electron injectors

    International Nuclear Information System (INIS)

    Sheffield, R.L.

    1987-01-01

    Free-electron laser (FEL) oscillators and synchrotron light sources require pulse trains of high peak brightness and, in some applications, high-average power. Recent developments in the technology of photoemissive and thermionic electron sources in rf cavities for electron-linac injector applications offer promising advances over conventional electron injectors. Reduced emittance growth in high peak-current electron injectors may be achieved by using high field strengths and by linearizing the radial component of the cavity electric field at the expense of lower shunt impedance

  4. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2016-01-01

    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses the foundati......Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  5. The lowest surface brightness disc galaxy known

    International Nuclear Information System (INIS)

    Davies, J.I.; Phillipps, S.; Disney, M.J.

    1988-01-01

    The discovery of a galaxy with a prominent bulge and a dominant extremely low surface brightness disc component is reported. The profile of this galaxy is very similar to the recently discovered giant low surface brightness galaxy Malin 1. The disc central surface brightness is found to be ∼ 26.4 Rμ, some 1.5 mag fainter than Malin 1 and thus by far the lowest yet observed. (author)

  6. Increasing the Brightness of Light Sources

    OpenAIRE

    Fu, Ling

    2006-01-01

    In modern illumination systems, compact size and high brightness are important features. Light recycling allows an increase of the spectral radiance (brightness) emitted by a light source for the price of reducing the total radiant power. Light recycling means returning part of the emitted light to the source where part of it will escape absorption. As a result, the output brightness can be increased in a restricted phase space, ...

  7. A Zn-porphyrin complex contributes to bright red color in Parma ham.

    Science.gov (United States)

    Wakamatsu, J; Nishimura, T; Hattori, A

    2004-05-01

    The Italian traditional dry-cured ham (Parma ham) shows a stable bright red color that is achieved without the use of nitrite and/or nitrate. In this study we examined the pigment spectroscopically, fluoroscopically and by using HPLC and ESI-HR-MASS analysis. Porphyrin derivative other than acid hematin were contained in the HCl-containing acetone extract from Parma ham. A strong fluorescence peak at 588 nm and a weak fluorescence peak at 641 nm were observed. By HPLC analysis the acetone extract of Parma ham was observed at the single peak, which eluted at the same time as Zn-protoporphyrin IX and emitted fluorescence. The results of ESI-HR-MS analysis showed both agreement with the molecular weight of Zn-protoporphyrin IX and the characteristic isotope pattern caused by Zn isotopes. These results suggest that the bright red color in Parma ham is caused by Zn-protoporphyrin IX.

  8. Confocal fluorescence techniques in industrial application

    Science.gov (United States)

    Eggeling, Christian; Gall, Karsten; Palo, Kaupo; Kask, Peet; Brand, Leif

    2003-06-01

    The FCS+plus family of evaluation tools for confocal fluorescence spectroscopy, which was developed during recent years, offers a comprehensive view to a series of fluorescence properties. Originating in fluorescence correlation spectroscopy (FCS) and using similar experimental equipment, a system of signal processing methods such as fluorescence intensity distribution analysis (FIDA) was created to analyze in detail the fluctuation behavior of fluorescent particles within a small area of detection. Giving simultaneous access to molecular parameters like concentration, translational and rotational diffusion, molecular brightness, and multicolor coincidence, this portfolio was enhanced by more traditional techniques of fluorescence lifetime as well as time-resolved anisotropy determination. The cornerstones of the FCS+plus methodology will be shortly described. The inhibition of a phosphatase enzyme activity gives a comprehensive industrial application that demonstrates FCS+plus' versatility and its potential for pharmaceutical drug discovery.

  9. The bright-bright and bright-dark mode coupling-based planar metamaterial for plasmonic EIT-like effect

    Science.gov (United States)

    Yu, Wei; Meng, Hongyun; Chen, Zhangjie; Li, Xianping; Zhang, Xing; Wang, Faqiang; Wei, Zhongchao; Tan, Chunhua; Huang, Xuguang; Li, Shuti

    2018-05-01

    In this paper, we propose a novel planar metamaterial structure for the electromagnetically induced transparency (EIT)-like effect, which consists of a split-ring resonator (SRR) and a pair of metal strips. The simulated results indicate that a single transparency window can be realized in the symmetry situation, which originates from the bright-bright mode coupling. Further, a dual-band EIT-like effect can be achieved in the asymmetry situation, which is due to the bright-bright mode coupling and bright-dark mode coupling, respectively. Different EIT-like effect can be simultaneously achieved in the proposed structure with the different situations. It is of certain significance for the study of EIT-like effect.

  10. Brightness and darkness as perceptual dimensions

    NARCIS (Netherlands)

    Vladusich, T.; Lucassen, M.P.; Cornelissen, F.W.

    2007-01-01

    A common-sense assumption concerning visual perception states that brightness and darkness cannot coexist at a given spatial location. One corollary of this assumption is that achromatic colors, or perceived grey shades, are contained in a one-dimensional (1-D) space varying from bright to dark. The

  11. SURFACE PHOTOMETRY OF LOW SURFACE BRIGHTNESS GALAXIES

    NARCIS (Netherlands)

    DEBLOK, WJG; VANDERHULST, JM; BOTHUN, GD

    1995-01-01

    Low surface brightness (LSB) galaxies are galaxies dominated by an exponential disc whose central surface brightness is much fainter than the value of mu(B)(0) = 21.65 +/- 0.30 mag arcsec(-2) found by Freeman. In this paper we present broadband photometry of a sample of 21 late-type LSB galaxies.

  12. Brightness Alteration with Interweaving Contours

    Directory of Open Access Journals (Sweden)

    Sergio Roncato

    2012-12-01

    Full Text Available Chromatic induction is observed whenever the perceived colour of a target surface shifts towards the hue of a neighbouring surface. Some vivid manifestations may be seen in a white background where thin coloured lines have been drawn (assimilation or when lines of different colours are collinear (neon effect or adjacent (watercolour to each other. This study examines a particular colour induction that manifests in concomitance with an opposite effect of colour saturation (or anti-spread. The two phenomena can be observed when a repetitive pattern is drawn in which outline thin contours intercept wider contours or surfaces, colour spreading appear to fill the surface occupied by surfaces or thick lines whereas the background traversed by thin lines is seen as brighter or filled of a saturated white. These phenomena were first observed by Bozzi (1975 and Kanizsa (1979 in figural conditions that did not allow them to document their conjunction. Here we illustrate various manifestations of this twofold phenomenon and compare its effects with the known effects of brightness and colour induction. Some conjectures on the nature of these effects are discussed.

  13. New Fluorescent Nanoparticles for Ultrasensitive Detection of Nucleic Acids by Optical Methods

    DEFF Research Database (Denmark)

    Mulberg, Mads Westergaard; Taskova, Maria; Thomsen, Rasmus P.

    2017-01-01

    in the synthesis of new fluorescent nanoparticles. Here, the fluorescent nanoparticles were made by simple and inexpensive radical emulsion polymerization of butyl acrylate in the presence of fluorescent dyes and additional functionalization reagents. This provided ultra-bright macrofluorophores of 9-84nm mean...

  14. Conjugated polymer dots for ultra-stable full-color fluorescence patterning.

    Science.gov (United States)

    Chang, Kaiwen; Liu, Zhihe; Chen, Haobin; Sheng, Lan; Zhang, Sean Xiao-An; Chiu, Daniel T; Yin, Shengyan; Wu, Changfeng; Qin, Weiping

    2014-11-12

    Stable full-color fluorescence patterning are achieved by multicolor polymer-dot inks. The fluorescent patterns show extraordinary stability upon various treatments, offering a superior combination of bright fluorescence, excellent photostability, chemical resistance, and eco-friendship. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Bright Sparks of Our Future!

    Science.gov (United States)

    Riordan, Naoimh

    2016-04-01

    My name is Naoimh Riordan and I am the Vice Principal of Rockboro Primary School in Cork City, South of Ireland. I am a full time class primary teacher and I teach 4th class, my students are aged between 9-10 years. My passion for education has developed over the years and grown towards STEM (Science, Technology, Engineering and Mathematics) subjects. I believe these subjects are the way forward for our future. My passion and beliefs are driven by the unique after school programme that I have developed. It is titled "Sparks" coming from the term Bright Sparks. "Sparks" is an after school programme with a difference where the STEM subjects are concentrated on through lessons such as Science, Veterinary Science Computer Animation /Coding, Eco engineering, Robotics, Magical Maths, Chess and Creative Writing. All these subjects are taught through activity based learning and are one-hour long each week for a ten-week term. "Sparks" is fully inclusive and non-selective which gives all students of any level of ability an opportunity to engage into these subjects. "Sparks" is open to all primary students in County Cork. The "Sparks" after school programme is taught by tutors from the different Universities and Colleges in Cork City. It works very well because the tutor brings their knowledge, skills and specialised equipment from their respective universities and in turn the tutor gains invaluable teaching practise, can trial a pilot programme in a chosen STEM subject and gain an insight into what works in the physical classroom.

  16. Designers predict a bright future

    International Nuclear Information System (INIS)

    Statton, T.D.

    1996-01-01

    As power plant designers and builders, there is a bright future for the industry. The demand for electricity will continue to grow, and the need for new plants will increase accordingly. But companies that develop and supply these plants must adapt to new ways of doing business if they expect to see the dawn of this new age. Several factors will have a profound effect on the generation and use of electricity in future years. Instant communications now reach all corners of the globe, making people everywhere aspire to a higher standard of living. The economic surge needed to satisfy these appetites will, in turn, be fed by a network of suppliers who are themselves restructuring to serve global markets, unimpeded by past nationalistic barriers to trade. The strong correlation between economic progress and the growing demand for electricity is well recognized. A ready supply of affordable electricity is a necessary underpinning for any economic expansion. As economies advance and jobs increase, electric demand grows geometrically, fueled by an ever-improving quality of life. Coupled with increasing demand is the worldwide trend toward privatization of the generation industry. The reasons may vary in different parts of the world, but the effect is the same--companies are battling intensely for the right to build or purchase generating facilities. Those companies, like the industry they serve, are themselves in a period of transition. Once a closed, monopolistic group of owners in a predominantly services-based market, they are, thanks to competitive forces, being driven steadily toward a product-based structure

  17. Bright boys the making of information technology

    CERN Document Server

    Green, Tom

    2010-01-01

    Everything has a beginning. None was more profound-and quite as unexpected-than Information Technology. Here for the first time is the untold story of how our new age came to be and the bright boys who made it happen. What began on the bare floor of an old laundry building eventually grew to rival in size the Manhattan Project. The unexpected consequence of that journey was huge---what we now know as Information Technology. For sixty years the bright boys have been totally anonymous while their achievements have become a way of life for all of us. "Bright Boys" brings them home. By 1950 they'd

  18. Effect of Surface Chemistry on the Fluorescence of Detonation Nanodiamonds.

    Science.gov (United States)

    Reineck, Philipp; Lau, Desmond W M; Wilson, Emma R; Fox, Kate; Field, Matthew R; Deeleepojananan, Cholaphan; Mochalin, Vadym N; Gibson, Brant C

    2017-11-28

    Detonation nanodiamonds (DNDs) have unique physical and chemical properties that make them invaluable in many applications. However, DNDs are generally assumed to show weak fluorescence, if any, unless chemically modified with organic molecules. We demonstrate that detonation nanodiamonds exhibit significant and excitation-wavelength-dependent fluorescence from the visible to the near-infrared spectral region above 800 nm, even without the engraftment of organic molecules to their surfaces. We show that this fluorescence depends on the surface functionality of the DND particles. The investigated functionalized DNDs, produced from the same purified DND as well as the as-received polyfunctional starting material, are hydrogen, hydroxyl, carboxyl, ethylenediamine, and octadecylamine-terminated. All DNDs are investigated in solution and on a silicon wafer substrate and compared to fluorescent high-pressure high-temperature nanodiamonds. The brightest fluorescence is observed from octadecylamine-functionalized particles and is more than 100 times brighter than the least fluorescent particles, carboxylated DNDs. The majority of photons emitted by all particle types likely originates from non-diamond carbon. However, we locally find bright and photostable fluorescence from nitrogen-vacancy centers in diamond in hydrogenated, hydroxylated, and carboxylated detonation nanodiamonds. Our results contribute to understanding the effects of surface chemistry on the fluorescence of DNDs and enable the exploration of the fluorescent properties of DNDs for applications in theranostics as nontoxic fluorescent labels, sensors, nanoscale tracers, and many others where chemically stable and brightly fluorescent nanoparticles with tailorable surface chemistry are needed.

  19. Time-resolved brightness measurements by streaking

    Science.gov (United States)

    Torrance, Joshua S.; Speirs, Rory W.; McCulloch, Andrew J.; Scholten, Robert E.

    2018-03-01

    Brightness is a key figure of merit for charged particle beams, and time-resolved brightness measurements can elucidate the processes involved in beam creation and manipulation. Here we report on a simple, robust, and widely applicable method for the measurement of beam brightness with temporal resolution by streaking one-dimensional pepperpots, and demonstrate the technique to characterize electron bunches produced from a cold-atom electron source. We demonstrate brightness measurements with 145 ps temporal resolution and a minimum resolvable emittance of 40 nm rad. This technique provides an efficient method of exploring source parameters and will prove useful for examining the efficacy of techniques to counter space-charge expansion, a critical hurdle to achieving single-shot imaging of atomic scale targets.

  20. BrightStat.com: free statistics online.

    Science.gov (United States)

    Stricker, Daniel

    2008-10-01

    Powerful software for statistical analysis is expensive. Here I present BrightStat, a statistical software running on the Internet which is free of charge. BrightStat's goals, its main capabilities and functionalities are outlined. Three different sample runs, a Friedman test, a chi-square test, and a step-wise multiple regression are presented. The results obtained by BrightStat are compared with results computed by SPSS, one of the global leader in providing statistical software, and VassarStats, a collection of scripts for data analysis running on the Internet. Elementary statistics is an inherent part of academic education and BrightStat is an alternative to commercial products.

  1. Measuring brightness temperature distributions of plasma bunches

    International Nuclear Information System (INIS)

    Kirko, V.I.; Stadnichenko, I.A.

    1981-01-01

    The possibility of restoration of brightness temperature distribution along plasma jet on the base of a simple ultra high- speed photography and subsequent photometric treatment is shown. The developed technique has been applied for finding spectral radiation intensity and brightness temperature of plasma jets of a tubular gas-cumulative charge and explosive plasma compressor. The problem of shock wave front has been successfully solved and thus distribution of above parameters beginning from the region preceeding the shock wave has been obtained [ru

  2. Bright THz Instrument and Nonlinear THz Science

    Science.gov (United States)

    2017-10-30

    Report: Bright THz Instrument and Nonlinear THz Science The views, opinions and/or findings contained in this report are those of the author(s) and...Number: W911NF-16-1-0436 Organization: University of Rochester Title: Bright THz Instrument and Nonlinear THz Science Report Term: 0-Other Email: xi...exploring new cutting-edge research and broader applications, following the significant development of THz science and technology in the late 80’s, is the

  3. The surface brightness of spiral galaxies

    International Nuclear Information System (INIS)

    Phillipps, S.; Disney, M.

    1983-01-01

    It is proposed that Freeman's discovery that the extrapolated central surface brightness of spiral galaxies is approximately constant can be simply explained if the galaxies contain a spheroidal component which dominates the light in their outer isophotes. Calculations of an effective central surface brightness indicate a wide spread of values. This requires either a wide spread in disc properties or significant spheroidal components or, most probably, both. (author)

  4. The surface brightness of spiral galaxies

    International Nuclear Information System (INIS)

    Disney, M.; Phillipps, S.

    1985-01-01

    The intrinsic surface brightness Ssub(e) of 500 disc galaxies (0<=T<=9) drawn from the Second Reference Catalogue is computed and it is shown that Ssub(e) does not correlate significantly with Msub(B), (B-V) or type. This is consistent with the notion that there is a heavy selection bias in favour of disc galaxies with that particular surface brightness which allows inclusion in the catalogue over the largest volume of space. (author)

  5. Brightness masking is modulated by disparity structure.

    Science.gov (United States)

    Pelekanos, Vassilis; Ban, Hiroshi; Welchman, Andrew E

    2015-05-01

    The luminance contrast at the borders of a surface strongly influences surface's apparent brightness, as demonstrated by a number of classic visual illusions. Such phenomena are compatible with a propagation mechanism believed to spread contrast information from borders to the interior. This process is disrupted by masking, where the perceived brightness of a target is reduced by the brief presentation of a mask (Paradiso & Nakayama, 1991), but the exact visual stage that this happens remains unclear. In the present study, we examined whether brightness masking occurs at a monocular-, or a binocular-level of the visual hierarchy. We used backward masking, whereby a briefly presented target stimulus is disrupted by a mask coming soon afterwards, to show that brightness masking is affected by binocular stages of the visual processing. We manipulated the 3-D configurations (slant direction) of the target and mask and measured the differential disruption that masking causes on brightness estimation. We found that the masking effect was weaker when stimuli had a different slant. We suggest that brightness masking is partly mediated by mid-level neuronal mechanisms, at a stage where binocular disparity edge structure has been extracted. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  6. A Zn-porphyrin complex contributes to bright red color in Parma ham

    OpenAIRE

    Wakamatsu, J.; Nishimura, T.; Hattori, A.

    2004-01-01

    The Italian traditional dry-cured ham (Parma ham) shows a stable bright red color that is achieved without the use of nitrite and/or nitrate. In this study we examined the pigment spectroscopically, fluoroscopically and by using HPLC and ESI-HR-MASS analysis. Porphyrin derivative other than acid hematin were contained in the HCl-containing acetone extract from Parma ham. A strong fluorescence peak at 588 nm and a weak fluorescence peak at 641 nm were observed. By HPLC analysis the acetone ext...

  7. Plasmonics Enhanced Smartphone Fluorescence Microscopy

    KAUST Repository

    Wei, Qingshan; Acuna, Guillermo; Kim, Seungkyeum; Vietz, Carolin; Tseng, Derek; Chae, Jongjae; Shir, Daniel; Luo, Wei; Tinnefeld, Philip; Ozcan, Aydogan

    2017-01-01

    Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.

  8. Plasmonics Enhanced Smartphone Fluorescence Microscopy

    KAUST Repository

    Wei, Qingshan

    2017-05-12

    Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.

  9. Bright perspectives for nuclear photonics

    Science.gov (United States)

    Thirolf, P. G.; Habs, D.

    2014-05-01

    With the advent of new high-power, short-pulse laser facilities in combination with novel technologies for the production of highly brilliant, intense γ beams (like, e.g., Extreme Light Infrastructure - Nuclear Physics (ELI-NP) in Bucharest, MEGaRay in Livermore or a planned upgrade of the HIγS facility at Duke University), unprecedented perspectives will open up in the coming years for photonuclear physics both in basic sciences as in various fields of applications. Ultra-high sensitivity will be enabled by an envisaged increase of the γ-beam spectral density from the presently typical 102γ/eVs to about 104γ/eVs, thus enabling a new quality of nuclear photonics [1], assisted by new γ-optical elements [2]. Photonuclear reactions with highly brilliant γ beams will allow to produce radioisotopes for nuclear medicine with much higher specific activity and/or more economically than with conventional methods. This will open the door for completely new clinical applications of radioisotopes [3]. The isotopic, state-selective sensitivity of the well-established technique of nuclear resonance fluorescence (NRF) will be boosted by the drastically reduced energy bandwidth (tomography and microscopy, assisting the management of nuclear materials, such as radioactive waste management, the detection of nuclear fissile material in the recycling process or the detection of clandestine fissile materials. Moreover, also secondary sources like low-energy, pulsed, polarized neutron beams of high intensity and high brilliance [4] or a new type of positron source with significantly increased brilliance, for the first time fully polarized [5], can be realized and lead to new applications in solid state physics or material sciences.

  10. Energy-exchange collisions of dark-bright-bright vector solitons.

    Science.gov (United States)

    Radhakrishnan, R; Manikandan, N; Aravinthan, K

    2015-12-01

    We find a dark component guiding the practically interesting bright-bright vector one-soliton to two different parametric domains giving rise to different physical situations by constructing a more general form of three-component dark-bright-bright mixed vector one-soliton solution of the generalized Manakov model with nine free real parameters. Moreover our main investigation of the collision dynamics of such mixed vector solitons by constructing the multisoliton solution of the generalized Manakov model with the help of Hirota technique reveals that the dark-bright-bright vector two-soliton supports energy-exchange collision dynamics. In particular the dark component preserves its initial form and the energy-exchange collision property of the bright-bright vector two-soliton solution of the Manakov model during collision. In addition the interactions between bound state dark-bright-bright vector solitons reveal oscillations in their amplitudes. A similar kind of breathing effect was also experimentally observed in the Bose-Einstein condensates. Some possible ways are theoretically suggested not only to control this breathing effect but also to manage the beating, bouncing, jumping, and attraction effects in the collision dynamics of dark-bright-bright vector solitons. The role of multiple free parameters in our solution is examined to define polarization vector, envelope speed, envelope width, envelope amplitude, grayness, and complex modulation of our solution. It is interesting to note that the polarization vector of our mixed vector one-soliton evolves in sphere or hyperboloid depending upon the initial parametric choices.

  11. Microwave-heating synthesis and sensing applications of bright gold nanoclusters

    Energy Technology Data Exchange (ETDEWEB)

    He, Ding-Fei; Xiang, Yang; Wang, Xu [Department of Physics, Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education and School of Physics and Technology, Wuhan University, Wuhan 430072 (China); Yu, Xue-Feng, E-mail: yxf@whu.edu.cn [Department of Physics, Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education and School of Physics and Technology, Wuhan University, Wuhan 430072 (China)

    2011-12-15

    Highlights: Black-Right-Pointing-Pointer We establish a microwave-heating method to synthesize protein-stabilized Au nanoclusters. Black-Right-Pointing-Pointer The obtained Au nanoclusters show bright red fluorescence. Black-Right-Pointing-Pointer The Au nanoclusters can be used as efficient fluorescence probe for Cu{sup 2+} ion sensing. -- Abstract: A rapid microwave-heating method has been developed for the synthesis of bright Au nanoclusters by using bull serum albumin as the template in an aqueous environment. The reaction time needed is only 7.0 min, and the weight of the products at one batch can reach 15 g. The Au nanoclusters exhibit bright fluorescence at {approx}613 nm with quantum yield of {approx}6.0%. By adjusting the pH value, the products can be controlled to precipitate or re-disperse in aqueous solution. Furthermore, the Au nanoclusters have exhibited high sensitivity and selectivity in the determination of Cu{sup 2+} ions in water. These results suggest an efficient method for obtaining metal nanoclusters for the detection and sensing applications.

  12. Unperturbed moderator brightness in pulsed neutron sources

    International Nuclear Information System (INIS)

    Batkov, K.; Takibayev, A.; Zanini, L.; Mezei, F.

    2013-01-01

    The unperturbed neutron brightness of a moderator can be defined from the number of neutrons leaving the surface of a moderator completely surrounded by a reflector. Without openings for beam extraction, it is the maximum brightness that can be theoretically achieved in a moderator. The unperturbed brightness of a cylindrical cold moderator filled with pure para-H 2 was calculated using MCNPX; the moderator dimensions were optimised, for a fixed target and reflector geometry corresponding to the present concept for the ESS spallation source. This quantity does not depend on openings for beam extraction and therefore can be used for a first-round optimisation of a moderator, before effects due to beam openings are considered. We find that such an optimisation yields to a factor of 2 increase with respect to a conventional volume moderator, large enough to accommodate a viewed surface of 12×12 cm 2 : the unperturbed neutron brightness is maximum for a disc-shaped moderator of 15 cm diameter, 1.4 cm height. The reasons for this increase can be related to the properties of the scattering cross-section of para-H 2 , to the added reflector around the exit surface in the case of a compact moderator, and to a directionality effect. This large optimisation gain in the unperturbed brightness hints towards similar potentials for the perturbed neutron brightness, in particular in conjunction with advancing the optical quality of neutron delivery from the moderator to the sample, where by Liouville theorem the brightness is conserved over the beam trajectory, except for absorption and similar type losses

  13. Intermittent episodes of bright light suppress myopia in the chicken more than continuous bright light.

    Science.gov (United States)

    Lan, Weizhong; Feldkaemper, Marita; Schaeffel, Frank

    2014-01-01

    Bright light has been shown a powerful inhibitor of myopia development in animal models. We studied which temporal patterns of bright light are the most potent in suppressing deprivation myopia in chickens. Eight-day-old chickens wore diffusers over one eye to induce deprivation myopia. A reference group (n = 8) was kept under office-like illuminance (500 lux) at a 10:14 light:dark cycle. Episodes of bright light (15 000 lux) were super-imposed on this background as follows. Paradigm I: exposure to constant bright light for either 1 hour (n = 5), 2 hours (n = 5), 5 hours (n = 4) or 10 hours (n = 4). Paradigm II: exposure to repeated cycles of bright light with 50% duty cycle and either 60 minutes (n = 7), 30 minutes (n = 8), 15 minutes (n = 6), 7 minutes (n = 7) or 1 minute (n = 7) periods, provided for 10 hours. Refraction and axial length were measured prior to and immediately after the 5-day experiment. Relative changes were analyzed by paired t-tests, and differences among groups were tested by one-way ANOVA. Compared with the reference group, exposure to continuous bright light for 1 or 2 hours every day had no significant protective effect against deprivation myopia. Inhibition of myopia became significant after 5 hours of bright light exposure but extending the duration to 10 hours did not offer an additional benefit. In comparison, repeated cycles of 1:1 or 7:7 minutes of bright light enhanced the protective effect against myopia and could fully suppress its development. The protective effect of bright light depends on the exposure duration and, to the intermittent form, the frequency cycle. Compared to the saturation effect of continuous bright light, low frequency cycles of bright light (1:1 min) provided the strongest inhibition effect. However, our quantitative results probably might not be directly translated into humans, but rather need further amendments in clinical studies.

  14. Crystal growth and characterization of fluorescent SiC

    DEFF Research Database (Denmark)

    Wellmann, P.; Kaiser, M.; Hupfer, T.

    -SiC co-doped with nitrogen and boron has been achieved [1][2]. The source is the rate determining step, and is expected to be determining the fluorescent properties by introducing dopants to the layer from the source. The optimization process of the polycrystalline, co-doped SiC:B,N source material...... and its impact on the FSPG epitaxial process, in particular the influence on the brightness of the is presented. In particular, the doping properties of the poly-SiC source material influence on the brightness of the fluorescent 6H-SiC. In addition we have investigated how the grain orientation...

  15. Proxy magnetometry of the photosphere: why are G-band bright points so bright?

    NARCIS (Netherlands)

    Rutten, R.J.; Kiselman, Dan; Voort, Luc Rouppe van der; Plez, Bertrand

    2000-01-01

    We discuss the formation of G-band bright points in terms of standard uxtube modeling, in particular the 1D LTE models constructed by Solanki and coworkers. Combined with LTE spectral synthesis they explain observed G-band bright point contrasts quite well. The G-band contrast increase over the

  16. A selective deficit in the appreciation and recognition of brightness: brightness agnosia?

    NARCIS (Netherlands)

    Nijboer, T.C.W.; Nys, G.M.S.; van der Smagt, M.J.; de Haan, E.H.F.

    2009-01-01

    We report a patient with extensive brain damage in the right hemisphere who demonstrated a severe impairment in the appreciation of brightness. Acuity, contrast sensitivity as well as luminance discrimination were normal, suggesting her brightness impairment is not a mere consequence of low-level

  17. A selective deficit in the appreciation and recognition of brightness: brightness agnosia?

    Science.gov (United States)

    Nijboer, Tanja C W; Nys, Gudrun M S; van der Smagt, Maarten J; de Haan, Edward H F

    2009-01-01

    We report a patient with extensive brain damage in the right hemisphere who demonstrated a severe impairment in the appreciation of brightness. Acuity, contrast sensitivity as well as luminance discrimination were normal, suggesting her brightness impairment is not a mere consequence of low-level sensory impairments. The patient was not able to indicate the darker or the lighter of two grey squares, even though she was able to see that they differed. In addition, she could not indicate whether the lights in a room were switched on or off, nor was she able to differentiate between normal greyscale images and inverted greyscale images. As the patient recognised objects, colours, and shapes correctly, the impairment is specific for brightness. As low-level, sensory processing is normal, this specific deficit in the recognition and appreciation of brightness appears to be of a higher, cognitive level, the level of semantic knowledge. This appears to be the first report of 'brightness agnosia'.

  18. Plasmonic EIT-like switching in bright-dark-bright plasmon resonators.

    Science.gov (United States)

    Chen, Junxue; Wang, Pei; Chen, Chuncong; Lu, Yonghua; Ming, Hai; Zhan, Qiwen

    2011-03-28

    In this paper we report the study of the electromagnetically induced transparency (EIT)-like transmission in the bright-dark-bright plasmon resonators. It is demonstrated that the interferences between the dark plasmons excited by two bright plasmon resonators can be controlled by the incident light polarization. The constructive interference strengthens the coupling between the bright and dark resonators, leading to a more prominent EIT-like transparency window of the metamaterial. In contrary, destructive interference suppresses the coupling between the bright and dark resonators, destroying the interference pathway that forms the EIT-like transmission. Based on this observation, the plasmonic EIT switching can be realized by changing the polarization of incident light. This phenomenon may find applications in optical switching and plasmon-based information processing.

  19. Fluorescence microscopy.

    Science.gov (United States)

    Sanderson, Michael J; Smith, Ian; Parker, Ian; Bootman, Martin D

    2014-10-01

    Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. In addition, the principles of how these microscopes form images are reviewed to appreciate their capabilities, limitations, and constraints for operation. © 2014 Cold Spring Harbor Laboratory Press.

  20. STARS4ALL Night Sky Brightness Photometer

    Directory of Open Access Journals (Sweden)

    Jaime Zamorano

    2017-06-01

    Full Text Available We present the main features of TESS-W, the first version of a series of inexpensive but reliable photometers that will be used to measure night sky brightness. The bandpass is extended to the red with respect of that of the Sky Quality Meter (SQM. TESS-W connects to a router via WIFI and it sends automatically the brightness values to a data repository using Internet of Things protocols. The device includes an infrared sensor to estimate the cloud coverage. It is designed for fixed stations to monitor the evolution of the sky brightness. The photometer could also be used in local mode connected to a computer or tablet to gather data from a moving vehicle. The photometer is being developed within STARS4ALL project, a collective awareness platform for promoting dark skies in Europe, funded by the EU. We intend to extend the existing professional networks to a citizen-based network of photometers. 

  1. Increasing the brightness of light sources

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Ling

    2006-11-16

    In this work the principle of light recycling is applied to artificial light sources in order to achieve brightness enhancement. Firstly, the feasibilities of increasing the brightness of light sources via light recycling are examined theoretically, based on the fundamental laws of thermodynamics including Kirchhoff's law on radiation, Planck's law, Lambert-Beer's law, the etendue conservation and the brightness theorem. From an experimental viewpoint, the radiation properties of three different kinds of light sources including short-arc lamps, incandescent lamps and LEDs characterized by their light-generating mechanisms are investigated. These three types of sources are used in light recycling experiments, for the purpose of 1. validating the intrinsic light recycling effect in light sources, e. g. the intrinsic light recycling effect in incandescent lamps stemming from the coiled filament structure. 2. acquiring the required parameters for establishing physical models, e.g. the emissivity/absorptivity of the short-arc lamps, the intrinsic reflectivity and the external quantum efficiency of LEDs. 3. laying the foundations for designing optics aimed at brightness enhancement according to the characteristics of the sources and applications. Based on the fundamental laws and experiments, two physical models for simulating the radiance distribution of light sources are established, one for thermal filament lamps, the other for luminescent sources, LEDs. As validation of the theoretical and experimental investigation of the light recycling effect, an optical device, the Carambola, is designed for achieving deterministic and multiple light recycling. The Carambola has the function of a concentrator. In order to achieve the maximum possible brightness enhancement with the Carambola, several combinations of sources and Carambolas are modelled in ray-tracing simulations. Sources with different light-emitting mechanisms and different radiation properties

  2. Richard Bright and his neurological studies.

    Science.gov (United States)

    Pearce, J M S

    2009-01-01

    Richard Bright was one of the famous triumvirate of Guy's Hospital physicians in the Victorian era. Remembered for his account of glomerulonephritis (Bright's disease) he also made many important and original contributions to medicine and neurology. These included his work on cortical epileptogenesis, descriptions of simple partial (Jacksonian) seizures, infantile convulsions, and a variety of nervous diseases. Most notable were his reports of neurological studies including papers on traumatic tetanus, syringomyelia, arteries of the brain, contractures of spinal origin, tumours of the base of the brain, and narcolepsy. His career and these contributions are outlined. Copyright 2009 S. Karger AG, Basel.

  3. Human CD56bright NK Cells

    DEFF Research Database (Denmark)

    Michel, Tatiana; Poli, Aurélie; Cuapio, Angelica

    2016-01-01

    Human NK cells can be subdivided into various subsets based on the relative expression of CD16 and CD56. In particular, CD56(bright)CD16(-/dim) NK cells are the focus of interest. They are considered efficient cytokine producers endowed with immunoregulatory properties, but they can also become c...... NK cell subsets is not fully defined, nor is their precise hematopoietic origin. In this article, we summarize recent studies about CD56(bright) NK cells in health and disease and briefly discuss the current controversies surrounding them....

  4. Diagnostics for high-brightness beams

    International Nuclear Information System (INIS)

    Shafer, R.E.

    1990-01-01

    Special techniques are required for beam diagnostics on high-brightness particle beams. Examples of high-brightness beams include low-emittance proton linacs (either pulsed or CW), electron linacs suitable for free-electron-laser applications, and future linear colliders. Non-interceptive and minimally-interceptive techniques for measuring beam current, position, profile, and transverse and longitudinal emittance will be reviewed. Included will be stripline, wire scanner, laser neutralization, beam-beam scattering, interceptive microgratings, spontaneous emission, optical transition radiation, and other techniques. 24 refs

  5. Use of astronomy filters in fluorescence microscopy.

    Science.gov (United States)

    Piper, Jörg

    2012-02-01

    Monochrome astronomy filters are well suited for use as excitation or suppression filters in fluorescence microscopy. Because of their particular optical design, such filters can be combined with standard halogen light sources for excitation in many fluorescent probes. In this "low energy excitation," photobleaching (fading) or other irritations of native specimens are avoided. Photomicrographs can be taken from living motile fluorescent specimens also with a flash so that fluorescence images can be created free from indistinctness caused by movement. Special filter cubes or dichroic mirrors are not needed for our method. By use of suitable astronomy filters, fluorescence microscopy can be carried out with standard laboratory microscopes equipped with condensers for bright-field (BF) and dark-field (DF) illumination in transmitted light. In BF excitation, the background brightness can be modulated in tiny steps up to dark or black. Moreover, standard industry microscopes fitted with a vertical illuminator for examinations of opaque probes in DF or BF illumination based on incident light (wafer inspections, for instance) can also be used for excitation in epi-illumination when adequate astronomy filters are inserted as excitatory and suppression filters in the illuminating and imaging light path. In all variants, transmission bands can be modulated by transmission shift.

  6. The Eindhoven High-Brightness Electron Programme

    NARCIS (Netherlands)

    Brussaard, G.J.H.; Wiel, van der M.J.

    2004-01-01

    The Eindhoven High-Brightness programme is aimed at producing ultra-short intense electron bunches from compact accelerators. The RF electron gun is capable of producing 100 fs electron bunches at 7.5 MeV and 10 pC bunch charge. The DC/RF hybrid gun under development will produce bunches <75 fs at

  7. Discussion of high brightness rf linear accelerators

    International Nuclear Information System (INIS)

    Jameson, R.A.

    1987-01-01

    The fundamental aspects of high-brightness rf linacs are outlined, showing the breadth and complexity of the technology and indicating that synergism with advancements in other areas is important. Areas of technology reviewed include ion sources, injectors, rf accelerator structures, beam dynamics, rf power, and automatic control

  8. Simultaneous brightness contrast of foraging Papilio butterflies

    Science.gov (United States)

    Kinoshita, Michiyo; Takahashi, Yuki; Arikawa, Kentaro

    2012-01-01

    This study focuses on the sense of brightness in the foraging Japanese yellow swallowtail butterfly, Papilio xuthus. We presented two red discs of different intensity on a grey background to butterflies, and trained them to select one of the discs. They were successfully trained to select either a high intensity or a low intensity disc. The trained butterflies were tested on their ability to perceive brightness in two different protocols: (i) two orange discs of different intensity presented on the same intensity grey background and (ii) two orange discs of the same intensity separately presented on a grey background that was either higher or lower in intensity than the training background. The butterflies trained to high intensity red selected the orange disc of high intensity in protocol 1, and the disc on the background of low intensity grey in protocol 2. We obtained similar results in another set of experiments with purple discs instead of orange discs. The choices of the butterflies trained to low intensity red were opposite to those just described. Taken together, we conclude that Papilio has the ability to learn brightness and darkness of targets independent of colour, and that they have the so-called simultaneous brightness contrast. PMID:22179808

  9. Robust fitting of diurnal brightness temperature cycle

    CSIR Research Space (South Africa)

    Udahemuka, G

    2007-11-01

    Full Text Available for a pixel concerned. Robust fitting of observed Diurnal Temperature Cycle (DTC) taken over a day of a given pixel without cloud cover and other abnormally conditions such as fire can give a data based brightness temperature model for a given pixel...

  10. Intermittent Episodes of Bright Light Suppress Myopia in the Chicken More than Continuous Bright Light

    OpenAIRE

    Lan, Weizhong; Feldkaemper, Marita; Schaeffel, Frank

    2014-01-01

    PURPOSE: Bright light has been shown a powerful inhibitor of myopia development in animal models. We studied which temporal patterns of bright light are the most potent in suppressing deprivation myopia in chickens. METHODS: Eight-day-old chickens wore diffusers over one eye to induce deprivation myopia. A reference group (n = 8) was kept under office-like illuminance (500 lux) at a 10:14 light:dark cycle. Episodes of bright light (15 000 lux) were super-imposed on this background as follows....

  11. Intermittent episodes of bright light suppress myopia in the chicken more than continuous bright light.

    Directory of Open Access Journals (Sweden)

    Weizhong Lan

    Full Text Available PURPOSE: Bright light has been shown a powerful inhibitor of myopia development in animal models. We studied which temporal patterns of bright light are the most potent in suppressing deprivation myopia in chickens. METHODS: Eight-day-old chickens wore diffusers over one eye to induce deprivation myopia. A reference group (n = 8 was kept under office-like illuminance (500 lux at a 10:14 light:dark cycle. Episodes of bright light (15 000 lux were super-imposed on this background as follows. Paradigm I: exposure to constant bright light for either 1 hour (n = 5, 2 hours (n = 5, 5 hours (n = 4 or 10 hours (n = 4. Paradigm II: exposure to repeated cycles of bright light with 50% duty cycle and either 60 minutes (n = 7, 30 minutes (n = 8, 15 minutes (n = 6, 7 minutes (n = 7 or 1 minute (n = 7 periods, provided for 10 hours. Refraction and axial length were measured prior to and immediately after the 5-day experiment. Relative changes were analyzed by paired t-tests, and differences among groups were tested by one-way ANOVA. RESULTS: Compared with the reference group, exposure to continuous bright light for 1 or 2 hours every day had no significant protective effect against deprivation myopia. Inhibition of myopia became significant after 5 hours of bright light exposure but extending the duration to 10 hours did not offer an additional benefit. In comparison, repeated cycles of 1:1 or 7:7 minutes of bright light enhanced the protective effect against myopia and could fully suppress its development. CONCLUSIONS: The protective effect of bright light depends on the exposure duration and, to the intermittent form, the frequency cycle. Compared to the saturation effect of continuous bright light, low frequency cycles of bright light (1:1 min provided the strongest inhibition effect. However, our quantitative results probably might not be directly translated into humans, but rather need further amendments in clinical studies.

  12. Circadian Phase-Shifting Effects of Bright Light, Exercise, and Bright Light + Exercise.

    Science.gov (United States)

    Youngstedt, Shawn D; Kline, Christopher E; Elliott, Jeffrey A; Zielinski, Mark R; Devlin, Tina M; Moore, Teresa A

    2016-02-26

    Limited research has compared the circadian phase-shifting effects of bright light and exercise and additive effects of these stimuli. The aim of this study was to compare the phase-delaying effects of late night bright light, late night exercise, and late evening bright light followed by early morning exercise. In a within-subjects, counterbalanced design, 6 young adults completed each of three 2.5-day protocols. Participants followed a 3-h ultra-short sleep-wake cycle, involving wakefulness in dim light for 2h, followed by attempted sleep in darkness for 1 h, repeated throughout each protocol. On night 2 of each protocol, participants received either (1) bright light alone (5,000 lux) from 2210-2340 h, (2) treadmill exercise alone from 2210-2340 h, or (3) bright light (2210-2340 h) followed by exercise from 0410-0540 h. Urine was collected every 90 min. Shifts in the 6-sulphatoxymelatonin (aMT6s) cosine acrophase from baseline to post-treatment were compared between treatments. Analyses revealed a significant additive phase-delaying effect of bright light + exercise (80.8 ± 11.6 [SD] min) compared with exercise alone (47.3 ± 21.6 min), and a similar phase delay following bright light alone (56.6 ± 15.2 min) and exercise alone administered for the same duration and at the same time of night. Thus, the data suggest that late night bright light followed by early morning exercise can have an additive circadian phase-shifting effect.

  13. Phosphorus ligand imaging with two-photon fluorescence spectroscopy: towards rational catalyst immobilization

    NARCIS (Netherlands)

    Marras, F.; Kluwer, A.M.; Siekierzycka, J.R.; Vozza, A.; Brouwer, A.M.; Reek, J.N.H.

    2010-01-01

    Spotless catalysts: Ligand immobilization was studied by two-photon fluorescence microscopy with a fluorescent nixantphos ligand as probe (see picture). In the immobilization process ligand aggregates form in solution and are deposited on the support, where they appear as bright spots in

  14. Cooperative effects and slow dynamics of fluorescence intensity from quantum emitters in a dielectric

    International Nuclear Information System (INIS)

    Lozing, N.A.; Gladush, M.G.

    2017-01-01

    We study theoretically the possibility of spontaneous switching between dim and bright fluorescence modes from a cooperative ensemble of two-level atoms driven by a cw-laser. A numerical analysis of transient regimes and transformations of the fluorescence spectrum are reported.

  15. Do Low Surface Brightness Galaxies Host Stellar Bars?

    Energy Technology Data Exchange (ETDEWEB)

    Cervantes Sodi, Bernardo; Sánchez García, Osbaldo, E-mail: b.cervantes@irya.unam.mx, E-mail: o.sanchez@irya.unam.mx [Instituto de Radioastronomía y Astrofísica, Universidad Nacional Autónoma de México, Campus Morelia, A.P. 3-72, C.P. 58089 Michoacán, México (Mexico)

    2017-09-20

    With the aim of assessing if low surface brightness galaxies host stellar bars and by studying the dependence of the occurrence of bars as a function of surface brightness, we use the Galaxy Zoo 2 data set to construct a large volume-limited sample of galaxies and then segregate these galaxies as having low or high surface brightness in terms of their central surface brightness. We find that the fraction of low surface brightness galaxies hosting strong bars is systematically lower than that found for high surface brightness galaxies. The dependence of the bar fraction on the central surface brightness is mostly driven by a correlation of the surface brightness with the spin and the gas richness of the galaxies, showing only a minor dependence on the surface brightness. We also find that the length of the bars is strongly dependent on the surface brightness, and although some of this dependence is attributed to the gas content, even at a fixed gas-to-stellar mass ratio, high surface brightness galaxies host longer bars than their low surface brightness counterparts, which we attribute to an anticorrelation of the surface brightness with the spin.

  16. Do Low Surface Brightness Galaxies Host Stellar Bars?

    International Nuclear Information System (INIS)

    Cervantes Sodi, Bernardo; Sánchez García, Osbaldo

    2017-01-01

    With the aim of assessing if low surface brightness galaxies host stellar bars and by studying the dependence of the occurrence of bars as a function of surface brightness, we use the Galaxy Zoo 2 data set to construct a large volume-limited sample of galaxies and then segregate these galaxies as having low or high surface brightness in terms of their central surface brightness. We find that the fraction of low surface brightness galaxies hosting strong bars is systematically lower than that found for high surface brightness galaxies. The dependence of the bar fraction on the central surface brightness is mostly driven by a correlation of the surface brightness with the spin and the gas richness of the galaxies, showing only a minor dependence on the surface brightness. We also find that the length of the bars is strongly dependent on the surface brightness, and although some of this dependence is attributed to the gas content, even at a fixed gas-to-stellar mass ratio, high surface brightness galaxies host longer bars than their low surface brightness counterparts, which we attribute to an anticorrelation of the surface brightness with the spin.

  17. Females are the brighter sex: Differences in external fluorescence across sexes and life stages of a crab spider.

    Science.gov (United States)

    Brandt, Erin E; Masta, Susan E

    2017-01-01

    Fluorescence is increasingly recognized to be widespread in nature. In particular, some arachnids fluoresce externally, and in spiders the hemolymph fluoresces. In this study, we examined the external fluorescence and the fluorophores of different sexes and life stages of the crab spider Misumena vatia (Clerk 1757), a sit-and-wait predator that feeds on insects as they visit flowers. We designed novel instrumentation to measure external fluorescence in whole specimens. We found that although males and females possess internal fluorophores with similar properties, the external expression of fluorescence varies across sexes and life stages. Spiders fluoresce brightly as immatures. Females maintain their brightness to adulthood, whereas males become increasingly dim as they mature. We suggest that external fluorescence likely contributes to visual signaling in these animals, and that it differs between the sexes as a result of differences in foraging ecology and behavior.

  18. Females are the brighter sex: Differences in external fluorescence across sexes and life stages of a crab spider.

    Directory of Open Access Journals (Sweden)

    Erin E Brandt

    Full Text Available Fluorescence is increasingly recognized to be widespread in nature. In particular, some arachnids fluoresce externally, and in spiders the hemolymph fluoresces. In this study, we examined the external fluorescence and the fluorophores of different sexes and life stages of the crab spider Misumena vatia (Clerk 1757, a sit-and-wait predator that feeds on insects as they visit flowers. We designed novel instrumentation to measure external fluorescence in whole specimens. We found that although males and females possess internal fluorophores with similar properties, the external expression of fluorescence varies across sexes and life stages. Spiders fluoresce brightly as immatures. Females maintain their brightness to adulthood, whereas males become increasingly dim as they mature. We suggest that external fluorescence likely contributes to visual signaling in these animals, and that it differs between the sexes as a result of differences in foraging ecology and behavior.

  19. Performance of LED fluorescence microscopy for the detection of ...

    African Journals Online (AJOL)

    Introduction: Ziehl-Neelsen (ZN) bright-field microscopy is time-consuming, with poor sensitivity, even under optimal conditions. Introduction of Primo Star iLED fluorescent microscopy (FM) may improve TB case finding at referral hospitals in Rwanda. The study aimed to determine the acceptability and effectiveness of iLED ...

  20. Fluorescent probe based on heteroatom containing styrylcyanine: pH-sensitive properties and bioimaging in vivo

    International Nuclear Information System (INIS)

    Yang, Xiaodong; Gao, Ya; Huang, Zhibing; Chen, Xiaohui; Ke, Zhiyong; Zhao, Peiliang; Yan, Yichen; Liu, Ruiyuan; Qu, Jinqing

    2015-01-01

    A novel fluorescent probe based on heteroatom containing styrylcyanine is synthesized. The fluorescence of probe is bright green in basic and neutral media but dark orange in strong acidic environments, which could be reversibly switched. Such behavior enables it to work as a fluorescent pH sensor in the solution state and a chemosensor for detecting acidic and basic volatile organic compounds. Analyses by NMR spectroscopy confirm that the protonation or deprotonation of pyridinyl moiety is responsible for the sensing process. In addition, the fluorescent microscopic images of probe in live cells and zebrafish are achieved successfully, suggesting that the probe has good cell membrane permeability and low cytotoxicity. - Graphical abstract: A novel styrylcyanine-based fluorescent pH probe was designed and synthesized, the fluorescence of which is bright green in basic and neutral media but dark orange in strong acidic environments. Such behavior enables it to work as a fluorescent pH sensor in solution states, and a chemosensor for detecting volatile organic compounds with high acidity and basicity in solid state. In addition, it can be used for fluorescent imaging in living cell and living organism. - Highlights: • Bright green fluorescence was observed in basic and neutral media. • Dark orange fluorescence was found in strong acidic environments. • Volatile organic compounds with high acidity and basicity could be detected. • Bioimaging in living cell and living organism was achieved successfully

  1. Bright solitons in Bose-Fermi mixtures

    International Nuclear Information System (INIS)

    Karpiuk, Tomasz; Brewczyk, Miroslaw; RzaPewski, Kazimierz

    2006-01-01

    We consider the formation of bright solitons in a mixture of Bose and Fermi degenerate gases confined in a three-dimensional elongated harmonic trap. The Bose and Fermi atoms are assumed to effectively attract each other whereas bosonic atoms repel each other. Strong enough attraction between bosonic and fermionic components can change the character of the interaction within the bosonic cloud from repulsive to attractive making thus possible the generation of bright solitons in the mixture. On the other hand, such structures might be in danger due to the collapse phenomenon existing in attractive gases. We show, however, that under some conditions (defined by the strength of the Bose-Fermi components attraction) the structures which neither spread nor collapse can be generated. For elongated enough traps the formation of solitons is possible even at the 'natural' value of the mutual Bose-Fermi ( 87 Rb- 40 K in our case) scattering length

  2. Extreme Ultraviolet Explorer Bright Source List

    Science.gov (United States)

    Malina, Roger F.; Marshall, Herman L.; Antia, Behram; Christian, Carol A.; Dobson, Carl A.; Finley, David S.; Fruscione, Antonella; Girouard, Forrest R.; Hawkins, Isabel; Jelinsky, Patrick

    1994-01-01

    Initial results from the analysis of the Extreme Ultraviolet Explorer (EUVE) all-sky survey (58-740 A) and deep survey (67-364 A) are presented through the EUVE Bright Source List (BSL). The BSL contains 356 confirmed extreme ultraviolet (EUV) point sources with supporting information, including positions, observed EUV count rates, and the identification of possible optical counterparts. One-hundred twenty-six sources have been detected longward of 200 A.

  3. High-brightness H/sup -/ accelerators

    International Nuclear Information System (INIS)

    Jameson, R.A.

    1987-01-01

    Neutral particle beam (NPB) devices based on high-brightness H/sup -/ accelerators are an important component of proposed strategic defense systems. The basic rational and R and D program are outlined and examples given of the underlying technology thrusts toward advanced systems. Much of the research accomplished in the past year is applicable to accelerator systems in general; some of these activities are discussed

  4. Brightness of synchrotron radiation from wigglers

    International Nuclear Information System (INIS)

    Geloni, Gianluca; Kocharyan, Vitali; Saldin, Evgeni

    2014-12-01

    According to literature, while calculating the brightness of synchrotron radiation from wigglers, one needs to account for the so called 'depth-of-field' effects. In fact, the particle beam cross section varies along the wiggler. It is usually stated that the effective photon source size increases accordingly, while the brightness is reduced. Here we claim that this is a misconception originating from an analysis of the wiggler source based on geometrical arguments, regarded as almost self-evident. According to electrodynamics, depth-of-field effects do not exist: we demonstrate this statement both theoretically and numerically, using a well-known first-principle computer code. This fact shows that under the usually accepted approximations, the description of the wiggler brightness turns out to be inconsistent even qualitatively. Therefore, there is a need for a well-defined procedure for computing the brightness from a wiggler source. We accomplish this task based on the use of a Wigner function formalism. In the geometrical optics limit computations can be performed analytically. Within this limit, we restrict ourselves to the case of the beam size-dominated regime, which is typical for synchrotron radiation facilities in the X-ray wavelength range. We give a direct demonstration of the fact that the apparent horizontal source size is broadened in proportion to the beamline opening angle and to the length of the wiggler. While this effect is well-understood, a direct proof appears not to have been given elsewhere. We consider the problem of the calculation of the wiggler source size by means of numerical simulations alone, which play the same role of an experiment. We report a significant numerical disagreement between exact calculations and approximations currently used in literature.

  5. Modular Zero Energy. BrightBuilt Home

    Energy Technology Data Exchange (ETDEWEB)

    Aldrich, Robb [Steven Winter Associates, Inc., Norwalk, CT (United States); Butterfield, Karla [Steven Winter Associates, Inc., Norwalk, CT (United States)

    2016-03-01

    With funding from the Building America Program, part of the U.S. Department of Energy Building Technologies Office, the Consortium for Advanced Residential Buildings (CARB) worked with BrightBuilt Home (BBH) to evaluate and optimize building systems. CARB’s work focused on a home built by Black Bros. Builders in Lincolnville, Maine (International Energy Conservation Code Climate Zone 6). As with most BBH projects to date, modular boxes were built by Keiser Homes in Oxford, Maine.

  6. The surface brightness of spiral galaxies: Pt. 4

    International Nuclear Information System (INIS)

    Phillipps, S.; Disney, M.; Ohio State Univ., Columbus

    1988-01-01

    Using measurements from IRAS correlations are found between optical surface brightness and both infrared-to-optical flux ratio and infrared colour temperature, in the sense that galaxies with high surface brightness have higher FIR emission and higher temperatures. (author)

  7. Search for bright stars with infrared excess

    Energy Technology Data Exchange (ETDEWEB)

    Raharto, Moedji, E-mail: moedji@as.itb.ac.id [Astronomy Research Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132 (Indonesia)

    2014-03-24

    Bright stars, stars with visual magnitude smaller than 6.5, can be studied using small telescope. In general, if stars are assumed as black body radiator, then the color in infrared (IR) region is usually equal to zero. Infrared data from IRAS observations at 12 and 25μm (micron) with good flux quality are used to search for bright stars (from Bright Stars Catalogues) with infrared excess. In magnitude scale, stars with IR excess is defined as stars with IR color m{sub 12}−m{sub 25}>0; where m{sub 12}−m{sub 25} = −2.5log(F{sub 12}/F{sub 25})+1.56, where F{sub 12} and F{sub 25} are flux density in Jansky at 12 and 25μm, respectively. Stars with similar spectral type are expected to have similar color. The existence of infrared excess in the same spectral type indicates the existence of circum-stellar dust, the origin of which is probably due to the remnant of pre main-sequence evolution during star formation or post AGB evolution or due to physical process such as the rotation of those stars.

  8. Condensate bright solitons under transverse confinement

    International Nuclear Information System (INIS)

    Salasnich, L.; Reatto, L.; Parola, A.

    2002-01-01

    We investigate the dynamics of Bose-Einstein condensate bright solitons made of alkali-metal atoms with negative scattering length and under harmonic confinement in the transverse direction. Contrary to the one-dimensional (1D) case, the 3D bright soliton exists only below a critical attractive interaction that depends on the extent of confinement. Such a behavior is also found in multisoliton condensates with box boundary conditions. We obtain numerical and analytical estimates of the critical strength beyond which the solitons do not exist. By using an effective 1D nonpolynomial nonlinear Schroedinger equation, which accurately takes into account the transverse dynamics of cigarlike condensates, we numerically simulate the dynamics of the 'soliton train' reported in a recent experiment [Nature (London) 417, 150 (2002)]. Then, analyzing the macroscopic quantum tunneling of the bright soliton on a Gaussian barrier, we find that its interference in the tunneling region is strongly suppressed with respect to nonsolitonic case; moreover, the tunneling through a barrier breaks the shape invariance of the matter wave. Finally, we show that the collapse of the soliton is induced by the scattering on the barrier or by the collision with another matter wave when the density reaches a critical value, for which we derive an accurate analytical formula

  9. Possible Bright Starspots on TRAPPIST-1

    Science.gov (United States)

    Morris, Brett M.; Agol, Eric; Davenport, James R. A.; Hawley, Suzanne L.

    2018-04-01

    The M8V star TRAPPIST-1 hosts seven roughly Earth-sized planets and is a promising target for exoplanet characterization. Kepler/K2 Campaign 12 observations of TRAPPIST-1 in the optical show an apparent rotational modulation with a 3.3-day period, though that rotational signal is not readily detected in the Spitzer light curve at 4.5 μm. If the rotational modulation is due to starspots, persistent dark spots can be excluded from the lack of photometric variability in the Spitzer light curve. We construct a photometric model for rotational modulation due to photospheric bright spots on TRAPPIST-1 that is consistent with both the Kepler and Spitzer light curves. The maximum-likelihood model with three spots has typical spot sizes of R spot/R ⋆ ≈ 0.004 at temperature T spot ≳ 5300 ± 200 K. We also find that large flares are observed more often when the brightest spot is facing the observer, suggesting a correlation between the position of the bright spots and flare events. In addition, these flares may occur preferentially when the spots are increasing in brightness, which suggests that the 3.3-day periodicity may not be a rotational signal, but rather a characteristic timescale of active regions.

  10. Beam brightness from a relativistic, field-emission diode with a velvet covered cathode

    International Nuclear Information System (INIS)

    Bekefi, G.; Shefer, R.E.; Tasker, S.C.

    1985-08-01

    The beam emittance and brightness from a mildly relativistic (200 to 400 kV) high current density (0.5 to 3.5kA/cm 2 ) planar, field emission diode provided with a velvet covered cathode have been studied experimentally as a function of the applied electric field (100 to 600kV/cm). Transverse beam spreading has been measured using a conventional pinhole arrangement followed by a fluorescent screen and open shutter camera. Good turn-on, and a high normalized beam brightness (B/sub n/ = 300kA/cm 2 -rad 2 ) have been observed. The results are compared with those obtained with a graphite cathode. 11 refs., 6 figs

  11. Star formation and the surface brightness of spiral galaxies

    International Nuclear Information System (INIS)

    Phillipps, S.; Disney, M.

    1985-01-01

    The (blue) surface brightness of spiral galaxies is significantly correlated with their Hα linewidth. This can be most plausibly interpreted as a correlation of surface brightness with star formation rate. There is also a significant difference in surface brightness between galaxies forming stars in a grand design spiral pattern and those with floc star formation regions. (author)

  12. Fluorescent polystyrene photonic crystals self-assembled with water-soluble conjugated polyrotaxanes

    Directory of Open Access Journals (Sweden)

    Francesco Di Stasio

    2013-10-01

    Full Text Available We demonstrate control of the photoluminescence spectra and decay rates of water-soluble green-emitting conjugated polyrotaxanes by incorporating them in polystyrene opals with a stop-band spectrally tuned on the rotaxane emission (405–650 nm. We observe a suppression of the luminescence within the photonic stop-band and a corresponding enhancement of the high-energy edge (405–447 nm. Time-resolved measurements reveal a wavelength-dependent modification of the emission lifetime, which is shortened at the high-energy edge (by ∼11%, in the range 405–447 nm, but elongated within the stop-band (by ∼13%, in the range 448–482 nm. We assign both effects to the modification of the density of photonic states induced by the photonic crystal band structure. We propose the growth of fluorescent composite photonic crystals from blends of “solvent-compatible” non-covalently bonded nanosphere-polymer systems as a general method for achieving a uniform distribution of polymeric dopants in three-dimensional self-assembling photonic structures.

  13. Steady full colour white organic light-emitting devices consisting of an ultrathin red fluorescent layer

    International Nuclear Information System (INIS)

    Wen Wen; Yu Junsheng; Li Lu; Wang Jun; Jiang Yadong

    2009-01-01

    White organic light-emitting devices were fabricated using an ultrathin red fluorescent dye of 3-(dicyanomethylene)-5, 5-dimethyl-1-(4-dimethylamino-styryl)cyclohexene inserted in tris(8-quinolinolato) aluminium layer as a red and green emitting layer (EML) and a thin 4, 4'-bis(2, 2'-diphenylvinyl)-1, 1'-diphenyl (DPVBi) layer as blue EML. A maximum power efficiency of 2.4 lm W -1 at 5.5 V and a maximum luminance of 16 690 cd m -2 at 18.5 V were obtained. Pure white emission with a good colour rendering index of 80 was achieved as low as 5 V. The Commission Internationale de l'Eclairage (CIE) coordinates near (0.330, 0.300) show a slight variation of (-0.020, +0.002) in a wide range of voltages. The achievement of full colour white emission at low-operation voltages and high-colour stability is attributed to the confining emission zone function of the thin EML and direct carrier trapping in the ultrathin layer.

  14. Steady full colour white organic light-emitting devices consisting of an ultrathin red fluorescent layer

    Energy Technology Data Exchange (ETDEWEB)

    Wen Wen; Yu Junsheng; Li Lu; Wang Jun; Jiang Yadong [State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054 (China)], E-mail: jsyu@uestc.edu.cn

    2009-01-07

    White organic light-emitting devices were fabricated using an ultrathin red fluorescent dye of 3-(dicyanomethylene)-5, 5-dimethyl-1-(4-dimethylamino-styryl)cyclohexene inserted in tris(8-quinolinolato) aluminium layer as a red and green emitting layer (EML) and a thin 4, 4'-bis(2, 2'-diphenylvinyl)-1, 1'-diphenyl (DPVBi) layer as blue EML. A maximum power efficiency of 2.4 lm W{sup -1} at 5.5 V and a maximum luminance of 16 690 cd m{sup -2} at 18.5 V were obtained. Pure white emission with a good colour rendering index of 80 was achieved as low as 5 V. The Commission Internationale de l'Eclairage (CIE) coordinates near (0.330, 0.300) show a slight variation of (-0.020, +0.002) in a wide range of voltages. The achievement of full colour white emission at low-operation voltages and high-colour stability is attributed to the confining emission zone function of the thin EML and direct carrier trapping in the ultrathin layer.

  15. Controlling excitons. Concepts for phosphorescent organic LEDs at high brightness

    Energy Technology Data Exchange (ETDEWEB)

    Reineke, Sebastian

    2009-11-15

    This work focusses on the high brightness performance of phosphorescent organic light-emitting diodes (OLEDs). The use of phosphorescent emitter molecules in OLEDs is essential to realize internal electron-photon conversion efficiencies of 100 %. However, due to their molecular nature, the excited triplet states have orders of magnitude longer time constants compared to their fluorescent counterparts which, in turn, strongly increases the probability of bimolecular annihilation. As a consequence, the efficiencies of phosphorescent OLEDs decline at high brightness - an effect known as efficiency roll-off, for which it has been shown to be dominated by triplet-triplet annihilation (TTA). In this work, TTA of the archetype phosphorescent emitter Ir(ppy){sub 3} is investigated in time-resolved photoluminescence experiments. For the widely used mixed system CBP:Ir(ppy){sub 3}, host-guest TTA - an additional unwanted TTA channel - is experimentally observed at high excitation levels. By using matrix materials with higher triplet energies, this effect is efficiently suppressed, however further studies show that the efficiency roll-off of Ir(ppy)3 is much more pronounced than predicted by a model based on Foerster-type energy transfer, which marks the intrinsic limit for TTA. These results suggest that the emitter molecules show a strong tendency to form aggregates in the mixed film as the origin for enhanced TTA. Transmission electron microscopy images of Ir(ppy){sub 3} doped mixed films give direct proof of emitter aggregates. Based on these results, two concepts are developed that improve the high brightness performance of OLEDs. In a first approach, thin intrinsic matrix interlayers are incorporated in the emission layer leading to a one-dimensional exciton confinement that suppresses exciton migration and, consequently, TTA. The second concept reduces the efficiency roll-off by using an emitter molecule with slightly different chemical structure, i.e. Ir(ppy){sub 2

  16. Problems of fluorescent imaging and its solution using nanofluorophores. Part I: Advantages of fluorescent nanoparticles over conventional organic fluorophores

    International Nuclear Information System (INIS)

    Zhelev, Z.; Hadjidekov, G.; Zlateva, G.; Spasov, L.; Bakalova, R.

    2011-01-01

    The application of fluorescence in deep-tissue imaging is rapidly expanding in fast several years. The progress in fluorescent molecular probes and fluorescent imaging techniques gives an opportunity to detect single cells and even molecules in live organisms. The highly sensitive and high-speed fluorescent molecular sensors and detection devices allow the application of fluorescence in functional imaging. With development of novel bright fluorophores based on nano-technologies and fluorescence scanners with high spatial and temporal resolution, the fluorescent imaging has a potential to become an alternative of the other non-invasive imaging techniques as magnetic resonance imaging, positron-emission tomography, X-ray, computing tomography. This review outlines the current status and future trends of fluorescent nanoparticles - quantum dots (QDs), as a new generation of fluorophores in experimental and pre-clinical fluorescent imaging diagnostic. Part 1 focuses on the advantages of quantum dots over conventional organic fluorophores and defines the major requirements to the 'perfect' fluorophore for fluorescent deep-tissue imaging diagnostic. The analysis is based on the limitations of fluorescent imaging in vivo and overcome by using quantum dots

  17. Electromagnetically induced transparency control in terahertz metasurfaces based on bright-bright mode coupling

    Science.gov (United States)

    Yahiaoui, R.; Burrow, J. A.; Mekonen, S. M.; Sarangan, A.; Mathews, J.; Agha, I.; Searles, T. A.

    2018-04-01

    We demonstrate a classical analog of electromagnetically induced transparency (EIT) in a highly flexible planar terahertz metamaterial (MM) comprised of three-gap split-ring resonators. The keys to achieve EIT in this system are the frequency detuning and hybridization processes between two bright modes coexisting in the same unit cell as opposed to bright-dark modes. We present experimental verification of two bright modes coupling for a terahertz EIT-MM in the context of numerical results and theoretical analysis based on a coupled Lorentz oscillator model. In addition, a hybrid variation of the EIT-MM is proposed and implemented numerically to dynamically tune the EIT window by incorporating photosensitive silicon pads in the split gap region of the resonators. As a result, this hybrid MM enables the active optical control of a transition from the on state (EIT mode) to the off state (dipole mode).

  18. Facile consecutive solvothermal growth of highly fluorescent InP/ZnS core/shell quantum dots using a safer phosphorus source.

    Science.gov (United States)

    Byun, Ho-June; Song, Woo-Seuk; Yang, Heesun

    2011-06-10

    The work presents a facile, stepwise synthetic approach for the production of highly fluorescent InP/ZnS core/shell quantum dots (QDs) by using a safer phosphorus (P) precursor. First, InP quantum dots (QDs) were solvothermally prepared at 180 °C for 24 h by using a P source of P(N(CH(3))(2))(3). The as-grown InP QDs were consecutively placed in another solvothermal condition for ZnS shell overcoating. In contrast to the almost non-fluorescent InP QDs, due to their highly defective surface states, the ZnS-coated InP QDs were highly fluorescent as a result of effective surface passivation. After the shell growth, the resulting InP/ZnS core/shell QDs were subjected to a size-sorting processing, by which red- to green-emitting QDs with quantum yields (QYs) of 24-60% were produced. Solvothermal shell growth parameters such as the reaction time and Zn/In solution concentration ratio were varied and optimized toward the highest QYs of core/shell QDs.

  19. Facile consecutive solvothermal growth of highly fluorescent InP/ZnS core/shell quantum dots using a safer phosphorus source

    International Nuclear Information System (INIS)

    Byun, Ho-June; Song, Woo-Seuk; Yang, Heesun

    2011-01-01

    The work presents a facile, stepwise synthetic approach for the production of highly fluorescent InP/ZnS core/shell quantum dots (QDs) by using a safer phosphorus (P) precursor. First, InP quantum dots (QDs) were solvothermally prepared at 180 deg. C for 24 h by using a P source of P(N(CH 3 ) 2 ) 3 . The as-grown InP QDs were consecutively placed in another solvothermal condition for ZnS shell overcoating. In contrast to the almost non-fluorescent InP QDs, due to their highly defective surface states, the ZnS-coated InP QDs were highly fluorescent as a result of effective surface passivation. After the shell growth, the resulting InP/ZnS core/shell QDs were subjected to a size-sorting processing, by which red- to green-emitting QDs with quantum yields (QYs) of 24-60% were produced. Solvothermal shell growth parameters such as the reaction time and Zn/In solution concentration ratio were varied and optimized toward the highest QYs of core/shell QDs.

  20. Facile consecutive solvothermal growth of highly fluorescent InP/ZnS core/shell quantum dots using a safer phosphorus source

    Science.gov (United States)

    Byun, Ho-June; Song, Woo-Seuk; Yang, Heesun

    2011-06-01

    The work presents a facile, stepwise synthetic approach for the production of highly fluorescent InP/ZnS core/shell quantum dots (QDs) by using a safer phosphorus (P) precursor. First, InP quantum dots (QDs) were solvothermally prepared at 180 °C for 24 h by using a P source of P(N(CH3)2)3. The as-grown InP QDs were consecutively placed in another solvothermal condition for ZnS shell overcoating. In contrast to the almost non-fluorescent InP QDs, due to their highly defective surface states, the ZnS-coated InP QDs were highly fluorescent as a result of effective surface passivation. After the shell growth, the resulting InP/ZnS core/shell QDs were subjected to a size-sorting processing, by which red- to green-emitting QDs with quantum yields (QYs) of 24-60% were produced. Solvothermal shell growth parameters such as the reaction time and Zn/In solution concentration ratio were varied and optimized toward the highest QYs of core/shell QDs.

  1. Facile consecutive solvothermal growth of highly fluorescent InP/ZnS core/shell quantum dots using a safer phosphorus source

    Energy Technology Data Exchange (ETDEWEB)

    Byun, Ho-June; Song, Woo-Seuk; Yang, Heesun, E-mail: hyang@hongik.ac.kr [Department of Materials Science and Engineering, Hongik University, Seoul 121-791 (Korea, Republic of)

    2011-06-10

    The work presents a facile, stepwise synthetic approach for the production of highly fluorescent InP/ZnS core/shell quantum dots (QDs) by using a safer phosphorus (P) precursor. First, InP quantum dots (QDs) were solvothermally prepared at 180 deg. C for 24 h by using a P source of P(N(CH{sub 3}){sub 2}){sub 3}. The as-grown InP QDs were consecutively placed in another solvothermal condition for ZnS shell overcoating. In contrast to the almost non-fluorescent InP QDs, due to their highly defective surface states, the ZnS-coated InP QDs were highly fluorescent as a result of effective surface passivation. After the shell growth, the resulting InP/ZnS core/shell QDs were subjected to a size-sorting processing, by which red- to green-emitting QDs with quantum yields (QYs) of 24-60% were produced. Solvothermal shell growth parameters such as the reaction time and Zn/In solution concentration ratio were varied and optimized toward the highest QYs of core/shell QDs.

  2. Investigating the Bright End of LSST Photometry

    Science.gov (United States)

    Ojala, Elle; Pepper, Joshua; LSST Collaboration

    2018-01-01

    The Large Synoptic Survey Telescope (LSST) will begin operations in 2022, conducting a wide-field, synoptic multiband survey of the southern sky. Some fraction of objects at the bright end of the magnitude regime observed by LSST will overlap with other wide-sky surveys, allowing for calibration and cross-checking between surveys. The LSST is optimized for observations of very faint objects, so much of this data overlap will be comprised of saturated images. This project provides the first in-depth analysis of saturation in LSST images. Using the PhoSim package to create simulated LSST images, we evaluate saturation properties of several types of stars to determine the brightness limitations of LSST. We also collect metadata from many wide-field photometric surveys to provide cross-survey accounting and comparison. Additionally, we evaluate the accuracy of the PhoSim modeling parameters to determine the reliability of the software. These efforts will allow us to determine the expected useable data overlap between bright-end LSST images and faint-end images in other wide-sky surveys. Our next steps are developing methods to extract photometry from saturated images.This material is based upon work supported in part by the National Science Foundation through Cooperative Agreement 1258333 managed by the Association of Universities for Research in Astronomy (AURA), and the Department of Energy under Contract No. DE-AC02-76SF00515 with the SLAC National Accelerator Laboratory. Additional LSST funding comes from private donations, grants to universities, and in-kind support from LSSTC Institutional Members.Thanks to NSF grant PHY-135195 and the 2017 LSSTC Grant Award #2017-UG06 for making this project possible.

  3. Selection effects in the bivariate brightness distribution for spiral galaxies

    International Nuclear Information System (INIS)

    Phillipps, S.; Disney, M.

    1986-01-01

    The joint distribution of total luminosity and characteristic surface brightness (the bivariate brightness distribution) is investigated for a complete sample of spiral galaxies in the Virgo cluster. The influence of selection and physical limits of various kinds on the apparent distribution are detailed. While the distribution of surface brightness for bright galaxies may be genuinely fairly narrow, faint galaxies exist right across the (quite small) range of accessible surface brightnesses so no statement can be made about the true extent of the distribution. The lack of high surface brightness bright galaxies in the Virgo sample relative to an overall RC2 sample (mostly field galaxies) supports the contention that the star-formation rate is reduced in the inner region of the cluster for environmental reasons. (author)

  4. Raman beam combining for laser brightness enhancement

    Science.gov (United States)

    Dawson, Jay W.; Allen, Graham S.; Pax, Paul H.; Heebner, John E.; Sridharan, Arun K.; Rubenchik, Alexander M.; Barty, Chrisopher B. J.

    2015-10-27

    An optical source capable of enhanced scaling of pulse energy and brightness utilizes an ensemble of single-aperture fiber lasers as pump sources, with each such fiber laser operating at acceptable pulse energy levels. Beam combining involves stimulated Raman scattering using a Stokes' shifted seed beam, the latter of which is optimized in terms of its temporal and spectral properties. Beams from fiber lasers can thus be combined to attain pulses with peak energies in excess of the fiber laser self-focusing limit of 4 MW while retaining the advantages of a fiber laser system of high average power with good beam quality.

  5. Bright emission lines in new Seyfert galaxies

    International Nuclear Information System (INIS)

    Afanasev, V.L.; Denisiuk, E.K.; Lipovetskii, V.A.; Shapovalova, A.I.

    1983-01-01

    Observational data are given on bright emission lines (H-alpha, H-beta, and forbidden N II, S II, and O III) for 14 recently discovered Seyfert galaxies. The investigated objects can be divided into three groups, which correspond approximately to the first (5 objects), the intermediate (4 objects), and the second (4 objects) Seyfert types. Attention is drawn to the properties of the galaxy Markaryan 1018, which has features of both the first and the second type and is distinguished by the weakness of its emission lines, which is probably due to a gas deficit. 7 references

  6. Next generation diode lasers with enhanced brightness

    Science.gov (United States)

    Ried, S.; Rauch, S.; Irmler, L.; Rikels, J.; Killi, A.; Papastathopoulos, E.; Sarailou, E.; Zimer, H.

    2018-02-01

    High-power diode lasers are nowadays well established manufacturing tools in high power materials processing, mainly for tactile welding, surface treatment and cladding applications. Typical beam parameter products (BPP) of such lasers range from 30 to 50 mm·mrad at several kilowatts of output power. TRUMPF offers a product line of diode lasers to its customers ranging from 150 W up to 6 kW of output power. These diode lasers combine high reliability with small footprint and high efficiency. However, up to now these lasers are limited in brightness due to the commonly used spatial and coarse spectral beam combining techniques. Recently diode lasers with enhanced brightness have been presented by use of dense wavelength multiplexing (DWM). In this paper we report on TRUMPF's diode lasers utilizing DWM. We demonstrate a 2 kW and a 4 kW system ideally suited for fine welding and scanner welding applications. The typical laser efficiency is in the range of 50%. The system offers plug and play exchange of the fiber beam delivery cable, multiple optical outputs and integrated cooling in a very compact package. An advanced control system offers flexible integration in any customer's shop floor environment and includes industry 4.0 capabilities (e.g. condition monitoring and predictive maintenance).

  7. Bright point study. [of solar corona

    Science.gov (United States)

    Tang, F.; Harvey, K.; Bruner, M.; Kent, B.; Antonucci, E.

    1982-01-01

    Transition region and coronal observations of bright points by instruments aboard the Solar Maximum Mission and high resolution photospheric magnetograph observations on September 11, 1980 are presented. A total of 31 bipolar ephemeral regions were found in the photosphere from birth in 9.3 hours of combined magnetograph observations from three observatories. Two of the three ephemeral regions present in the field of view of the Ultraviolet Spectrometer-Polarimeter were observed in the C IV 1548 line. The unobserved ephemeral region was determined to be the shortest-lived (2.5 hr) and lowest in magnetic flux density (13G) of the three regions. The Flat Crystal Spectrometer observed only low level signals in the O VIII 18.969 A line, which were not statistically significant to be positively identified with any of the 16 ephemeral regions detected in the photosphere. In addition, the data indicate that at any given time there lacked a one-to-one correspondence between observable bright points and photospheric ephemeral regions, while more ephemeral regions were observed than their counterparts in the transition region and the corona.

  8. Brightness and transparency in the early visual cortex.

    Science.gov (United States)

    Salmela, Viljami R; Vanni, Simo

    2013-06-24

    Several psychophysical studies have shown that transparency can have drastic effects on brightness and lightness. However, the neural processes generating these effects have remained unresolved. Several lines of evidence suggest that the early visual cortex is important for brightness perception. While single cell recordings suggest that surface brightness is represented in the primary visual cortex, the results of functional magnetic resonance imaging (fMRI) studies have been discrepant. In addition, the location of the neural representation of transparency is not yet known. We investigated whether the fMRI responses in areas V1, V2, and V3 correlate with brightness and transparency. To dissociate the blood oxygen level-dependent (BOLD) response to brightness from the response to local border contrast and mean luminance, we used variants of White's brightness illusion, both opaque and transparent, in which luminance increments and decrements cancel each other out. The stimuli consisted of a target surface and a surround. The surround luminance was always sinusoidally modulated at 0.5 Hz to induce brightness modulation to the target. The target luminance was constant or modulated in counterphase to null brightness modulation. The mean signal changes were calculated from the voxels in V1, V2, and V3 corresponding to the retinotopic location of the target surface. The BOLD responses were significantly stronger for modulating brightness than for stimuli with constant brightness. In addition, the responses were stronger for transparent than for opaque stimuli, but there was more individual variation. No interaction between brightness and transparency was found. The results show that the early visual areas V1-V3 are sensitive to surface brightness and transparency and suggest that brightness and transparency are represented separately.

  9. Strategies for the design of bright upconversion nanoparticles for bioanalytical applications

    Science.gov (United States)

    Wiesholler, Lisa M.; Hirsch, Thomas

    2018-06-01

    In recent years upconversion nanoparticles (UCNPs) received great attention because of their outstanding optical properties. Especially in bioanalytical applications this class of materials can overcome limitations of common probes like high background fluorescence or blinking. Nevertheless, the requirements for UCNPs to be applicable in biological samples, e.g. small size, water-dispersibility, excitation at low power density are in contradiction with the demand of high brightness. Therefore, a lot of attention is payed to the enhancement of the upconversion luminescence. This review discuss the recent trends and strategies to boost the brightness of UCNPs, classified in three main directions: a) improving the efficiency of energy absorption by the sensitizer via coupling to plasmonic or photonic structures or via attachment of ligands for light harvesting; b) minimizing non-radiative deactivation by variations in the architecture of UCNPs; and c) changing the excitation wavelength to get bright particles at low excitation power density for applications in aqueous systems. These strategies are critically reviewed including current limitations as well as future perspectives for the design of efficient UCNPs especially for sensing application in biological samples or cells.

  10. Carbon "Quantum" Dots for Fluorescence Labeling of Cells.

    Science.gov (United States)

    Liu, Jia-Hui; Cao, Li; LeCroy, Gregory E; Wang, Ping; Meziani, Mohammed J; Dong, Yiyang; Liu, Yuanfang; Luo, Pengju G; Sun, Ya-Ping

    2015-09-02

    The specifically synthesized and selected carbon dots of relatively high fluorescence quantum yields were evaluated in their fluorescence labeling of cells. For the cancer cell lines, the cellular uptake of the carbon dots was generally efficient, resulting in the labeling of the cells with bright fluorescence emissions for both one- and two-photon excitations from predominantly the cell membrane and cytoplasm. In the exploration on labeling the live stem cells, the cellular uptake of the carbon dots was relatively less efficient, though fluorescence emissions could still be adequately detected in the labeled cells, with the emissions again predominantly from the cell membrane and cytoplasm. This combined with the observed more efficient internalization of the same carbon dots by the fixed stem cells might suggest some significant selectivity of the stem cells toward surface functionalities of the carbon dots. The needs and possible strategies for more systematic and comparative studies on the fluorescence labeling of different cells, including especially live stem cells, by carbon dots as a new class of brightly fluorescent probes are discussed.

  11. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

    NARCIS (Netherlands)

    Mastop, M.; Bindels, D.S.; Shaner, N.C.; Postma, M.; Gadella, T.W.J.; Goedhart, J.

    2017-01-01

    The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and photostability, which are dependent on the characteristics of the fluorescent proteins that are employed. Yellow fluorescent protein (YFP) is often used as an acceptor but YFP is prone to photobleaching

  12. Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection.

    Science.gov (United States)

    Yan, Shengyong; Huang, Rong; Zhou, Yangyang; Zhang, Ming; Deng, Minggang; Wang, Xiaolin; Weng, Xiaocheng; Zhou, Xiang

    2011-01-28

    In this thrombin detection system, the bright fluorescence of TASPI is almost eliminated by the DNA aptamer TBA (turn-off); however, in the presence of thrombin, it specifically binds to TBA by folding unrestricted TBA into an anti-parallel G-quadruplex structure and then releasing TASPI molecules, resulting in vivid and facile fluorescence recovery (turn-on).

  13. FAA Fluorescent Penetrant Laboratory Inspections

    Energy Technology Data Exchange (ETDEWEB)

    WINDES,CONNOR L.; MOORE,DAVID G.

    2000-08-02

    The Federal Aviation Administration Airworthiness Assurance NDI Validation Center currently assesses the capability of various non-destructive inspection (NDI) methods used for analyzing aircraft components. The focus of one such exercise is to evaluate the sensitivity of fluorescent liquid penetrant inspection. A baseline procedure using the water-washable fluorescent penetrant method defines a foundation for comparing the brightness of low cycle fatigue cracks in titanium test panels. The analysis of deviations in the baseline procedure will determine an acceptable range of operation for the steps in the inspection process. The data also gives insight into the depth of each crack and which step(s) of the inspection process most affect penetrant sensitivities. A set of six low cycle fatigue cracks produced in 6.35-mm thick Ti-6Al-4V specimens was used to conduct the experiments to produce sensitivity data. The results will document the consistency of the crack readings and compare previous experiments to find the best parameters for water-washable penetrant.

  14. Very-High-Brightness Picosecond Electron Source

    International Nuclear Information System (INIS)

    Bluem, H.

    2003-01-01

    Bright, RF photocathode electron guns are the source of choice for most high-performance research accelerator applications. Some of these applications are pushing the performance boundaries of the present state-of-the-art guns. Advanced Energy Systems is developing a novel photocathode RF gun that shows excellent promise for extending gun performance. Initial gun simulations with only a short booster accelerator easily break the benchmark emittance of one micron for 1 nC of bunch charge. The pulse length in these simulations is less than 2 ps. It is expected that with more detailed optimization studies, the performance can be further improved. The performance details of the gun will be presented. In addition, we will discuss the present design concept along with the status of the project

  15. New redshifts of bright galaxies. III

    International Nuclear Information System (INIS)

    de Vaucouleurs, G.; de Vaucouleurs, A.; Nieto, J.

    1979-01-01

    Redshifts of 196 bright galaxies, and 2 QSO's are derived from 246 spectrograms obtained from 1972 to 1977 with the Galaxy meter's two-state image tube grism spectrograph attached at the Cassegrain focus of the McDonald Observatory Struve reflector. The reciprocal dispersion in 335 A/mm at Hα and the wavelength range lambdalambda 4500--8000 A. The galaxy redshifts are in the range -28 -1 , but few exceed 5,000 km s -1 . The internal mean errors of the weighted mean velocities range from 22 to 140 km s -1 . Comparisons with other systems of redshifts, particularly the RC2, 21-cm and Sandage systems, indicate a mean systematic error of -35 +- 16 km s -1 , but it is probably variable with V. The external mean error is sigma*=90 km s -1 for velocities V -1 having a mean weight =4.0

  16. Dark Skies, Bright Kids Year 8

    Science.gov (United States)

    Bittle, Lauren E.; Wenger, Trey; Johnson, Kelsey E.; Angell, Dylan; Burkhardt, Andrew; Davis, Blair; Firebaugh, Ariel; Hancock, Danielle; Richardson, Whitney; Rochford Hayes, Christian; Linden, Sean; Liss, Sandra; Matthews, Allison; McNair, Shunlante; Prager, Brian; Pryal, Matthew; Troup, Nicholas William

    2017-01-01

    We present activities from the eighth year of Dark Skies Bright Kids (DSBK), an entirely volunteer-run outreach organization based out of the Department of Astronomy at the University of Virginia. Our core mission is to enhance elementary science education and literacy in Central Virginia through fun, hands-on activities that introduce basic Astronomy concepts. Over the past seven years, our primary focus has been hosting an 8-10 week after-school astronomy club at underserved elementary and middle schools, and over the past several years, we have partnered with local businesses to host our Annual Central Virginia Star Party, a free event open to the community featuring star-gazing and planetarium shows. This past summer we expanded our reach through a new initiative to bring week-long summer day camps to south and southwest Virginia, home to some of the most underserved communities in the commonwealth.

  17. Dark Skies, Bright Kids Year 7

    Science.gov (United States)

    Bittle, Lauren E.; Johnson, Kelsey E.; Borish, H. Jacob; Burkhardt, Andrew; Firebaugh, Ariel; Hancock, Danielle; Rochford Hayes, Christian; Linden, Sean; Liss, Sandra; Matthews, Allison; Prager, Brian; Pryal, Matthew; Sokal, Kimberly R.; Troup, Nicholas William; Wenger, Trey

    2016-01-01

    We present updates from our seventh year of operation including new club content, continued assessments, and our fifth annual Star Party. Dark Skies, Bright Kids (DSBK) is an entirely volunteer-run outreach organization based out of the Department of Astronomy at the University of Virginia. Our core mission is to enhance elementary science education and literacy in Central Virginia through fun, hands-on activities that introduce basic Astronomy concepts. Our primary focus is hosting an 8-10 week after-school astronomy club at underserved elementary and middle schools. Each week, DSBK volunteers take the role of coaches to introduce astronomy-related concepts ranging from the Solar System to galaxies to astrobiology, and to lead students in interactive learning activities. Another hallmark of DSBK is hosting our Annual Central Virginia Star Party, a free event open to the community featuring star-gazing and planetarium shows.

  18. Considerations for high-brightness electron sources

    International Nuclear Information System (INIS)

    Jameson, R.A.

    1990-01-01

    Particle accelerators are now used in many areas of physics research and in industrial and medical applications. New uses are being studied to address major societal needs in energy production, materials research, generation of intense beams of radiation at optical and suboptical wavelengths, treatment of various kinds of waste, and so on. Many of these modern applications require a high intensity beam at the desired energy, along with a very good beam quality in terms of the beam confinement, aiming, or focusing. Considerations for ion and electron accelerators are often different, but there are also many commonalties, and in fact, techniques derived for one should perhaps more often be considered for the other as well. We discuss some aspects of high-brightness electron sources here from that point of view. 6 refs

  19. Life-threatening motor vehicle crashes in bright sunlight

    OpenAIRE

    Redelmeier, Donald A.; Raza, Sheharyar

    2017-01-01

    Abstract Bright sunlight may create visual illusions that lead to driver error, including fallible distance judgment from aerial perspective. We tested whether the risk of a life-threatening motor vehicle crash was increased when driving in bright sunlight. This longitudinal, case-only, paired-comparison analysis evaluated patients hospitalized because of a motor vehicle crash between January 1, 1995 and December 31, 2014. The relative risk of a crash associated with bright sunlight was estim...

  20. Fluorescence properties of 6-aryl-2‧-deoxy-furanouridine and -pyrrolocytidine and their derivatives

    Science.gov (United States)

    Ro, Jong Jin; Go, Gui Han; Wilhelmsson, L. Marcus; Hyean Kim, Byeang

    2018-01-01

    2‧-deoxyfuranouridine derivatives presenting various aryl groups have been synthesized through Cu(I)-catalyzed intramolecular cyclizations. Moreover, corresponding pyrrolo-dC derivatives have been synthesized and both families of compounds thoroughly characterized using UV/vis and fluorescence spectroscopy as well as time-dependent density functional theory calculations. The photophysical characterization, show that our newly synthesized derivatives of the important pyrrolo-dC family have high fluorescence quantum yields (QYs) and brightness values. Pyrrolo-dC derivative, 3a, shows an environment sensitive QY of up to >60% and brightness of almost 3000, in low polarity solvents and excitation and emission maxima between 365-381 nm and 479-510 nm, respectively, in solvents of different polarities. Two other derivatives, 3b and 3c, show high QYs and brightness values of up to 3300 that are fairly insensitive to their microenvironment. These promising photophysical features suggest future applicability as fluorescent nucleobase analogs.

  1. Study of Three-Dimensional Image Brightness Loss in Stereoscopy

    Directory of Open Access Journals (Sweden)

    Hsing-Cheng Yu

    2015-10-01

    Full Text Available When viewing three-dimensional (3D images, whether in cinemas or on stereoscopic televisions, viewers experience the same problem of image brightness loss. This study aims to investigate image brightness loss in 3D displays, with the primary aim being to quantify the image brightness degradation in the 3D mode. A further aim is to determine the image brightness relationship to the corresponding two-dimensional (2D images in order to adjust the 3D-image brightness values. In addition, the photographic principle is used in this study to measure metering values by capturing 2D and 3D images on television screens. By analyzing these images with statistical product and service solutions (SPSS software, the image brightness values can be estimated using the statistical regression model, which can also indicate the impact of various environmental factors or hardware on the image brightness. In analysis of the experimental results, comparison of the image brightness between 2D and 3D images indicates 60.8% degradation in the 3D image brightness amplitude. The experimental values, from 52.4% to 69.2%, are within the 95% confidence interval

  2. The night sky brightness at McDonald Observatory

    Science.gov (United States)

    Kalinowski, J. K.; Roosen, R. G.; Brandt, J. C.

    1975-01-01

    Baseline observations of the night sky brightness in B and V are presented for McDonald Observatory. In agreement with earlier work by Elvey and Rudnick (1937) and Elvey (1943), significant night-to-night and same-night variations in sky brightness are found. Possible causes for these variations are discussed. The largest variation in sky brightness found during a single night is approximately a factor of two, a value which corresponds to a factor-of-four variation in airglow brightness. The data are used to comment on the accuracy of previously published surface photometry of M 81.

  3. Diversity and Ecological Correlates of Red Fluorescence in Marine Fishes

    Directory of Open Access Journals (Sweden)

    Nils Anthes

    2016-11-01

    Full Text Available Marine environments at depths below -10 to -25 m are almost devoid of ambient red sunlight because water quickly attenuates long wavelengths. This stenospectral light environment presents unique opportunities for organisms that can transform ambient blue-green light into red light by fluorescence. Numerous marine fish species display intricate patterns of fluorescence. Because color vision is a key component of fish sensory ecology, several putative visual functions of red fluorescence have been proposed but are difficult to test experimentally. Here, we follow a comparative approach to assess the consistency between the phylogenetic distribution of red fluorescence with its presumed functions. We collected and analyzed the largest data set of red fluorescence in fishes to date, consisting of confirmed cases in 272 primarily diurnal fish species from 49 out of 90 surveyed fish families and 12 out of 21 surveyed fish orders, contrasted to 393 fish species with confirmed absence of red fluorescence. Based on a priori hypotheses on adaptive function, we compare the prevalence of red fluorescence among pre-defined sets of species based on ecological or biological characteristics while controlling for shared ancestry. When comparing between species, we find no evidence that red fluorescence is more prevalent in deep-water species, contrasting with our recent finding that fluorescence brightness increases with depth within species. There is also no evidence for a role in group-driven communication. Phylogenetic patterns are consistent, however, with three other predictions. First, fluorescence with a rather patchy distribution across the body occurred significantly more often among sit-and-wait predators or otherwise sedentary fish than in more mobile species, consistent with background matching for camouflage. Second, small, predatory fishes tended to show red fluorescent irides disproportionally often consistent with a proposed function in prey

  4. Automated local bright feature image analysis of nuclear protein distribution identifies changes in tissue phenotype

    International Nuclear Information System (INIS)

    Knowles, David; Sudar, Damir; Bator, Carol; Bissell, Mina

    2006-01-01

    The organization of nuclear proteins is linked to cell and tissue phenotypes. When cells arrest proliferation, undergo apoptosis, or differentiate, the distribution of nuclear proteins changes. Conversely, forced alteration of the distribution of nuclear proteins modifies cell phenotype. Immunostaining and fluorescence microscopy have been critical for such findings. However, there is an increasing need for quantitative analysis of nuclear protein distribution to decipher epigenetic relationships between nuclear structure and cell phenotype, and to unravel the mechanisms linking nuclear structure and function. We have developed imaging methods to quantify the distribution of fluorescently-stained nuclear protein NuMA in different mammary phenotypes obtained using three-dimensional cell culture. Automated image segmentation of DAPI-stained nuclei was generated to isolate thousands of nuclei from three-dimensional confocal images. Prominent features of fluorescently-stained NuMA were detected using a novel local bright feature analysis technique, and their normalized spatial density calculated as a function of the distance from the nuclear perimeter to its center. The results revealed marked changes in the distribution of the density of NuMA bright features as non-neoplastic cells underwent phenotypically normal acinar morphogenesis. In contrast, we did not detect any reorganization of NuMA during the formation of tumor nodules by malignant cells. Importantly, the analysis also discriminated proliferating non-neoplastic cells from proliferating malignant cells, suggesting that these imaging methods are capable of identifying alterations linked not only to the proliferation status but also to the malignant character of cells. We believe that this quantitative analysis will have additional applications for classifying normal and pathological tissues

  5. Automated local bright feature image analysis of nuclear proteindistribution identifies changes in tissue phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Knowles, David; Sudar, Damir; Bator, Carol; Bissell, Mina

    2006-02-01

    The organization of nuclear proteins is linked to cell and tissue phenotypes. When cells arrest proliferation, undergo apoptosis, or differentiate, the distribution of nuclear proteins changes. Conversely, forced alteration of the distribution of nuclear proteins modifies cell phenotype. Immunostaining and fluorescence microscopy have been critical for such findings. However, there is an increasing need for quantitative analysis of nuclear protein distribution to decipher epigenetic relationships between nuclear structure and cell phenotype, and to unravel the mechanisms linking nuclear structure and function. We have developed imaging methods to quantify the distribution of fluorescently-stained nuclear protein NuMA in different mammary phenotypes obtained using three-dimensional cell culture. Automated image segmentation of DAPI-stained nuclei was generated to isolate thousands of nuclei from three-dimensional confocal images. Prominent features of fluorescently-stained NuMA were detected using a novel local bright feature analysis technique, and their normalized spatial density calculated as a function of the distance from the nuclear perimeter to its center. The results revealed marked changes in the distribution of the density of NuMA bright features as non-neoplastic cells underwent phenotypically normal acinar morphogenesis. In contrast, we did not detect any reorganization of NuMA during the formation of tumor nodules by malignant cells. Importantly, the analysis also discriminated proliferating non-neoplastic cells from proliferating malignant cells, suggesting that these imaging methods are capable of identifying alterations linked not only to the proliferation status but also to the malignant character of cells. We believe that this quantitative analysis will have additional applications for classifying normal and pathological tissues.

  6. Bright environments vision of the Intelligent Lighting Institute (ILI)

    NARCIS (Netherlands)

    Özçelebi, T.

    2014-01-01

    The Bright Environments research program of the Eindhoven University of Technology Intelligent Lighting Institute aims to find new methods of intelligent lighting control and human interaction. We present a summary of the institute’s work on this research field and the research vision of the Bright

  7. Spatially single-mode source of bright squeezed vacuum

    OpenAIRE

    Pérez, A. M.; Iskhakov, T. Sh.; Sharapova, P.; Lemieux, S.; Tikhonova, O. V.; Chekhova, M. V.; Leuchs, G.

    2014-01-01

    Bright squeezed vacuum, a macroscopic nonclassical state of light, can be obtained at the output of a strongly pumped non-seeded traveling-wave optical parametric amplifier (OPA). By constructing the OPA of two consecutive crystals separated by a large distance we make the squeezed vacuum spatially single-mode without a significant decrease in the brightness or squeezing.

  8. The ASAS-SN bright supernova catalogue - III. 2016

    DEFF Research Database (Denmark)

    Holoien, T. W. -S.; Brown, J. S.; Stanek, K. Z.

    2017-01-01

    This catalogue summarizes information for all supernovae discovered by the All-Sky Automated Survey for SuperNovae (ASAS-SN) and all other bright (m(peak)d......This catalogue summarizes information for all supernovae discovered by the All-Sky Automated Survey for SuperNovae (ASAS-SN) and all other bright (m(peak)d...

  9. Analysis of Bright Harvest Remote Analysis for Residential Solar Installations

    Energy Technology Data Exchange (ETDEWEB)

    Nangle, John [National Renewable Energy Lab. (NREL), Golden, CO (United States); Simon, Joseph [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-06-17

    Bright Harvest provides remote shading analysis and design products for residential PV system installers. The National Renewable Energy Laboratory (NREL) through the NREL Commercialization Assistance Program, completed comparative assessments between on-site measurements and remotely calculated values to validate the accuracy of Bright Harvest’s remote shading and power generation.

  10. Dark and bright vortex solitons in electromagnetically induced transparent media

    International Nuclear Information System (INIS)

    Wu Xuan; Xie Xiaotao; Yang Xiaoxue

    2006-01-01

    We show that dark and bright vortex solitons can exist in three-state electromagnetically induced transparent media under some appropriate conditions. We also analyse the stability of the dark and bright vortex solitons. This work may provide other research opportunities in nonlinear optical experiments and may result in a substantial impact on technology

  11. Edge integration and the perception of brightness and darkness

    NARCIS (Netherlands)

    Vladusich, T.; Lucassen, M.P.; Cornelissen, F.W.

    2006-01-01

    How do induced brightness and darkness signals from local and remote surfaces interact to determine the final achromatic color percept of a target surface? An emerging theory of achromatic color perception posits that brightness and darkness percepts are computed by weighting and summing the

  12. Lamp spectrum and spatial brightness at photopic levels

    DEFF Research Database (Denmark)

    Fotios, Steve; Atli, Deniz; Cheal, Chris

    2015-01-01

    Light sources are available in a variety of spectral power distributions (SPDs) and this affects spatial brightness in a manner not predicted by quantities such as illuminance. Tuning light source SPD to better match the sensitivity of visual perception may allow the same spatial brightness but a...

  13. Morphing hydrogel patterns by thermo-reversible fluorescence switching.

    Science.gov (United States)

    Bat, Erhan; Lin, En-Wei; Saxer, Sina; Maynard, Heather D

    2014-07-01

    Stimuli responsive surfaces that show reversible fluorescence switching behavior in response to temperature changes were fabricated. Oligo(ethylene glycol) methacrylate thermoresponsive polymers with amine end-groups were prepared by atom transfer radical polymerization (ATRP). The polymers were patterned on silicon surfaces by electron beam (e-beam) lithography, followed by conjugation of self-quenching fluorophores. Fluorophore conjugated hydrogel thin films were bright when the gels were swollen; upon temperature-induced collapse of the gels, self-quenching of the fluorophores led to significant attenuation of fluorescence. Importantly, the fluorescence was regained when the temperature was cooled. The fluorescence switching behavior of the hydrogels for up to ten cycles was investigated and the swelling-collapse was verified by atomic force microscopy. Morphing surfaces that change shape several times upon increase in temperature were obtained by patterning multiple stimuli responsive polymers. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Brightness waves of electroluminescence in ZnO:La electroluminor

    International Nuclear Information System (INIS)

    Bhushan, S.; Pandey, A.N.; Kaza, Balakrishna Rao

    1979-01-01

    A cryostat for the measurement of different luminescent characteristics from liquid N 2 temperature to above has been fabricated. Using this cryostat brightness waves due to sinusoidal excitations for ZnO:La electroluminor (EL) has been studied at different temperatures from -168deg C. Brightness waves for this system consist of two primary peaks during each cycle of excitation. Each primary peak is associated with a secondary peak. This secondary peak at -168deg C exists at the left arm of the primary peak. As the temperature is increased to 18deg C it moves towards the right arm of the primary peak. At an intermediate temperature the secondary peaks are most pronounced. Possible mechanism for these phenomena have been discussed. Temperature dependence of time averaged EL brightness for this system has also been studied and three peaks have been found. The results of brightness waves have also been discussed in the light of temperature dependence of time averaged EL brightness. (auth.)

  15. Intrinsic brightness temperatures of blazar jets at 15 GHz

    Directory of Open Access Journals (Sweden)

    Hovatta Talvikki

    2013-12-01

    Full Text Available We have developed a new Bayesian Markov Chain Monte Carlo method to deconvolve light curves of blazars into individual flares, including proper estimation of the fit errors. We use the method to fit 15GHzlight curves obtained within the OVRO 40-m blazar monitoring program where a large number of AGN have been monitored since 2008 in support of the Fermi Gamma-Ray Space Telescope mission. The time scales obtained from the fitted models are used to calculate the variability brightness temperature of the sources. Additionally, we have calculated brightness temperatures of a sample of these objects using Very Long Baseline Array data from the MOJAVE survey. Combining these two data sets enables us to study the intrinsic brightness temperature distribution in these blazars at 15 GHz. Our preliminary results indicate that the mean intrinsic brightness temperature in a sample of 14 sources is near the equipartition brightness temperature of ~ 1011K.

  16. Intercomparisons of nine sky brightness detectors.

    Science.gov (United States)

    den Outer, Peter; Lolkema, Dorien; Haaima, Marty; van der Hoff, Rene; Spoelstra, Henk; Schmidt, Wim

    2011-01-01

    Nine Sky Quality Meters (SQMs) have been intercompared during a night time measurement campaign held in the Netherlands in April 2011. Since then the nine SQMs have been distributed across The Netherlands and form the Dutch network for monitoring night sky brightness. The goal of the intercomparison was to infer mutual calibration factors and obtain insight into the variability of the SQMs under different meteorological situations. An ensemble average is built from the individual measurements and used as a reference to infer the mutual calibration factors. Data required additional synchronization prior to the calibration determination, because the effect of moving clouds combined with small misalignments emerges as time jitter in the measurements. Initial scatter of the individual instruments lies between ±14%. Individual night time sums range from -16% to +20%. Intercalibration reduces this to 0.5%, and -7% to +9%, respectively. During the campaign the smallest luminance measured was 0.657 ± 0.003 mcd/m(2) on 12 April, and the largest value was 5.94 ± 0.03 mcd/m(2) on 2 April. During both occurrences interfering circumstances like snow cover or moonlight were absent.

  17. Dark Skies, Bright Kids Year 9

    Science.gov (United States)

    Burkhardt, Andrew Michael; Matthews, Allison M.; Johnson, Kelsey E.; Avilez, Ian; Beale, Luca; Bittle, Lauren E.; Bordenave, David; Finn, Molly; Firebaugh, Ariel; Hancock, Danielle; Hughes, Paul; Rochford Hayes, Christian; Lewis, Hannah; Linden, Sean; Liss, Sandra; Liu, Mengyao; McNair, Shunlante; Murphy, Edward; Prager, Brian; Pryal, Matthew; Richardson, Whitney; Song, Yiqing; Troup, Nicholas; Villadsen, Jackie; Wenger, Trey V.; Wilson, Robert Forrest

    2018-01-01

    We present updates from the ninth year of operation of Dark Skies, Bright Kids (DSBK) including new club content, continued assessments, and our seventh annual Star Party. DSBK is an entirely volunteer-run outreach organization based out of the Department of Astronomy at the University of Virginia. Our core mission is to enhance elementary science education and literacy in Virginia through fun, hands-on activities that introduce basic Astronomy concepts. DSBK’s most fundamental program is an 8-10 week long after-school Astronomy camp at surrounding local elementary schools, where each week introduces new concepts through interactive hands-on activities. Over the past two summers, we have traveled to four rural Virginia locations to bring week-long Astronomy camps to otherwise overlooked elementary school districts. These programs aim to inspire a curiosity for science and include inquiry based activities in topics ranging from the electromagnetic spectrum to the classification and evolution of galaxies. We strive to be self-reflective in our mission to inspire scientific curiosity in the minds of underserved demographics. In this effort, we continually assess the effectiveness of each activity through feedback in student-kept journal pages and observed excitement levels. This self-reflection has initiated the development of new curriculum. In addition, differing from our normal collaboration with local elementary schools, we have found great success partnering with local youth organizations, who may better represent DSBK's target demographics and have infrastructure to support incoming outreach groups.

  18. Enhanced brightness x-ray lasers

    International Nuclear Information System (INIS)

    Wan, A.S.; Cauble, R.C.; Da Silva, L.B.; Moreno, J.C.; Nilsen, J.

    1994-09-01

    We are developing short-pulsed, enhanced-brightness, and coherent x-ray lasers (XRLs) for applications in areas such as plasma imaging. In a traveling wave pump setup the optical laser creating the XRL plasma sweeps along the lasant axis at the same speed as the x-rays. This technique becomes increasingly important as the target length increases and the gain duration shortens. An order of magnitude increase in output energy was measured with vs without traveling wave pump. Using multiple pulse techniques and multilayer mirrors to inject the output of one pulse back into the plasma formed by a later pulse we have begun to develop the x-ray analog of a multi-pass amplifler. The use of multiple pulses separated by as much as 1.6 ns reduces multilayer mirror damage. This injection technique is demonstrated by imaging the near-field emission profiles of the XRL. The addition of multilayer beamsplitter will allow us to effectively produce a soft XRL cavity

  19. Featured Image: Bright Dots in a Sunspot

    Science.gov (United States)

    Kohler, Susanna

    2018-03-01

    This image of a sunspot, located in in NOAA AR 12227, was captured in December 2014 by the 0.5-meter Solar Optical Telescope on board the Hinode spacecraft. This image was processed by a team of scientists led by Rahul Yadav (Udaipur Solar Observatory, Physical Research Laboratory Dewali, India) in order to examine the properties of umbral dots: transient, bright features observed in the umbral region (the central, darkest part) of a sunspot. By exploring these dots, Yadav and collaborators learned how their properties relate to the large-scale properties of the sunspots in which they form for instance, how do the number, intensities, or filling factors of dots relate to the size of a sunspots umbra? To find out more about the authors results, check out the article below.Sunspot in NOAA AR 11921. Left: umbralpenumbral boundary. Center: the isolated umbra from the sunspot. Right: The umbra with locations of umbral dots indicated by yellow plus signs. [Adapted from Yadav et al. 2018]CitationRahul Yadav et al 2018 ApJ 855 8. doi:10.3847/1538-4357/aaaeba

  20. The Los Alamos high-brightness photoinjector

    Energy Technology Data Exchange (ETDEWEB)

    O' Shea, P.G.

    1991-01-01

    For a number of years Los Alamos National Laboratory has been developing photocathode RF guns for high-brightness electron beam applications such as free-electron lasers (FELs). Previously thermionic high-voltage guns have been the source of choice for the electron accelerators used to drive FELs. The performance of such FELs is severely limited by the emittance growth produced by the subharmonic bunching process and also by the low peak current of the source. In a photoinjector, a laser driven photocathode is placed directly in a high-gradient RF accelerating cavity. A photocathode allows unsurpassed control over the current, and the spatial and temporal profile of the beam. In addition the electrodeless emission'' avoids many of the difficulties associated with multi-electrode guns, i.e. the electrons are accelerated very rapidly to relativistic energies, and there are no electrodes to distort the accelerating fields. For the past two years we have been integrating a photocathode into our existing FEL facility by replacing our thermionic gun and subharmonic bunchers with a high-gradient 1.3 GHz photoinjector. The photoinjector, which is approximately 0.6 m in length, produces 6 MeV, 300 A, 15 ps linac, and accelerated to a final energy of 40 MeV. We have recently begun lasing at wavelengths near 3 {mu}m. 16 refs., 2 figs., 5 tabs.

  1. Modular Zero Energy. BrightBuilt Home

    Energy Technology Data Exchange (ETDEWEB)

    Aldrich, Robb [Consortium for Advanced Residential Buildings (CARB), Norwalk, CT (United States). Steven Winters Associates, Inc.; Butterfield, Karla [Consortium for Advanced Residential Buildings (CARB), Norwalk, CT (United States). Steven Winters Associates, Inc.

    2016-03-01

    Kaplan Thompson Architects (KTA) has specialized in sustainable, energy-efficient buildings, and they have designed several custom, zero-energy homes in New England. These zero-energy projects have generally been high-end, custom homes with budgets that could accommodate advanced energy systems. In an attempt to make zero energy homes more affordable and accessible to a larger demographic, KTA explored modular construction as way to provide high-quality homes at lower costs. In the mid-2013, KTA formalized this concept when they launched BrightBuilt Home (BBH). The BBH mission is to offer a line of architect-designed, high-performance homes that are priced to offer substantial savings off the lifetime cost of a typical home and can be delivered in less time. For the past two years, CARB has worked with BBH and Keiser Homes (the primary modular manufacturer for BBH) to discuss challenges related to wall systems, HVAC, and quality control. In Spring of 2014, CARB and BBH began looking in detail on a home to be built in Lincolnville, ME by Black Bros. Builders. This report details the solution package specified for this modular plan and the challenges that arose during the project.

  2. Intercomparisons of Nine Sky Brightness Detectors

    Directory of Open Access Journals (Sweden)

    Henk Spoelstra

    2011-10-01

    Full Text Available Nine Sky Quality Meters (SQMs have been intercompared during a night time measurement campaign held in the Netherlands in April 2011. Since then the nine SQMs have been distributed across the Netherlands and form the Dutch network for monitoring night sky brightness. The goal of the intercomparison was to infer mutual calibration factors and obtain insight into the variability of the SQMs under different meteorological situations. An ensemble average is built from the individual measurements and used as a reference to infer the mutual calibration factors. Data required additional synchronization prior to the calibration determination, because the effect of moving clouds combined with small misalignments emerges as time jitter in the measurements. Initial scatter of the individual instruments lies between ±14%. Individual night time sums range from −16% to +20%. Intercalibration reduces this to 0.5%, and −7% to +9%, respectively. During the campaign the smallest luminance measured was 0.657 ± 0.003 mcd/m2 on 12 April, and the largest value was 5.94 ± 0.03 mcd/m2 on 2 April. During both occurrences interfering circumstances like snow cover or moonlight were absent.

  3. Dark Skies, Bright Kids Year 6

    Science.gov (United States)

    Liss, Sandra; Troup, Nicholas William; Johnson, Kelsey E.; Barcos-Munoz, Loreto D.; Beaton, Rachael; Bittle, Lauren; Borish, Henry J.; Burkhardt, Andrew; Corby, Joanna; Dean, Janice; Hancock, Danielle; King, Jennie; Prager, Brian; Romero, Charles; Sokal, Kimberly R.; Stierwalt, Sabrina; Wenger, Trey; Zucker, Catherine

    2015-01-01

    Now entering our sixth year of operation, Dark Skies, Bright Kids (DSBK) is an entirely volunteer-run outreach organization based out of the Department of Astronomy at the University of Virginia. Our core mission is to enhance elementary science education and literacy in central Virginia through fun, hands-on activities that introduce basic Astronomy concepts beyond Virginia's Standards of Learning. Our primary focus is hosting an 8-10 week after-school astronomy club at underserved elementary and middle schools. Each week, DSBK volunteers take the role of coaches to introduce astronomy-related concepts ranging from the Solar System to galaxies to astrobiology, and to lead students in interactive learning activities. Another hallmark of DSBK is hosting our Annual Central Virginia Star Party, a free event open to the community featuring star-gazing and planetarium shows.DSBK has amassed over 15,000 contact hours since 2009 and we continue to broaden our impact. One important step we have taken in the past year is to establish a graduate student led assessment program to identify and implement directed learning goals for DSBK outreach. The collection of student workbooks, observations, and volunteer surveys indicates broad scale success for the program both in terms of student learning and their perception of science. The data also reveal opportunities to improve our organizational and educational practices to maximize student achievement and overall volunteer satisfaction for DSBK's future clubs and outreach endeavors.

  4. Reviews in fluorescence 2010

    CERN Document Server

    Geddes, Chris D

    2011-01-01

    ""Reviews in Fluorescence 2010"", the seventh volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year's progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. ""Reviews in Fluorescence"" offers an essential reference material for any lab working in the fluoresc

  5. Principles of fluorescence techniques

    CERN Document Server

    2016-01-01

    Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

  6. Bright fluorescent Streptococcus pneumoniae for live cell imaging of host-pathogen interactions

    NARCIS (Netherlands)

    Kjos, M.; Aprianto, R.; Fernandes, V.E.; Andrew, P.W.; Strijp, van J.A.G.; Nijland, R.; Veening, J.W.

    2015-01-01

    Streptococcus pneumoniae is a common nasopharyngeal resident in healthy people, but at the same time one of the major causes of infectious diseases such as pneumonia, meningitis and sepsis. The shift from commensal to pathogen and its interaction with host cells is poorly understood. One of the

  7. Bright Fluorescent Streptococcus pneumoniae for Live-Cell Imaging of Host-Pathogen Interactions

    NARCIS (Netherlands)

    Kjos, Morten; Aprianto, Rieza; Fernandes, Vitor E.; Andrew, Peter W.; van Strijp, Jos A. G.; Nijland, Reindert; Veening, Jan-Willem

    Streptococcus pneumoniae is a common nasopharyngeal resident in healthy people but, at the same time, one of the major causes of infectious diseases such as pneumonia, meningitis, and sepsis. The shift from commensal to pathogen and its interaction with host cells are poorly understood. One of the

  8. A Bright Future for Precision Medicine: Advances in Fluorescent Chemical Probe Design and Their Clinical Application.

    Science.gov (United States)

    Garland, Megan; Yim, Joshua J; Bogyo, Matthew

    2016-01-21

    The Precision Medicine Initiative aims to use advances in basic and clinical research to develop therapeutics that selectively target and kill cancer cells. Under the same doctrine of precision medicine, there is an equally important need to visualize these diseased cells to enable diagnosis, facilitate surgical resection, and monitor therapeutic response. Therefore, there is a great opportunity for chemists to develop chemically tractable probes that can image cancer in vivo. This review focuses on recent advances in the development of optical probes, as well as their current and future applications in the clinical management of cancer. The progress in probe development described here suggests that optical imaging is an important and rapidly developing field of study that encourages continued collaboration among chemists, biologists, and clinicians to further refine these tools for interventional surgical imaging, as well as for diagnostic and therapeutic applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Benchtop Fluorination of Fluorescent Nanodiamonds on a Preparative Scale: Toward Unusually Hydrophilic Bright Particles

    Czech Academy of Sciences Publication Activity Database

    Havlík, Jan; Raabová, Helena; Gulka, Michal; Petráková, Vladimíra; Krečmarová, M.; Mašek, V.; Louša, Petr; Štursa, Jan; Boyen, H. G.; Nesládek, M.; Cígler, Petr

    2016-01-01

    Roč. 26, č. 23 (2016), s. 4134-4142 ISSN 1616-301X R&D Projects: GA MZd(CZ) NV15-33094A; GA MŠk(CZ) LM2011019; GA MŠk(CZ) LO1304; GA ČR(CZ) GA16-16336S Institutional support: RVO:61388963 ; RVO:68378271 ; RVO:61389005 Keywords : nitrogen-vacancy centers * catalyzed decarboxylative fluorination * surface modification Subject RIV: CC - Organic Chemistry Impact factor: 12.124, year: 2016

  10. Fluorescent nanodiamonds embedded in biocompatible translucent shells.

    Science.gov (United States)

    Rehor, Ivan; Slegerova, Jitka; Kucka, Jan; Proks, Vladimir; Petrakova, Vladimira; Adam, Marie-Pierre; Treussart, François; Turner, Stuart; Bals, Sara; Sacha, Pavel; Ledvina, Miroslav; Wen, Amy M; Steinmetz, Nicole F; Cigler, Petr

    2014-03-26

    High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties of bare NDs limit their direct use in these applications: they precipitate in biological solutions, only a limited set of bio-orthogonal conjugation techniques is available and the accessible material is greatly polydisperse in shape. In this work, we encapsulate bright 30-nm fluorescent nanodiamonds (FNDs) in 10-20-nm thick translucent (i.e., not altering FND fluorescence) silica shells, yielding monodisperse near-spherical particles of mean diameter 66 nm. High yield modification of the shells with PEG chains stabilizes the particles in ionic solutions, making them applicable in biological environments. We further modify the opposite ends of PEG chains with fluorescent dyes or vectoring peptide using click chemistry. High conversion of this bio-orthogonal coupling yielded circa 2000 dye or peptide molecules on a single FND. We demonstrate the superior properties of these particles by in vitro interaction with human prostate cancer cells: while bare nanodiamonds strongly aggregate in the buffer and adsorb onto the cell membrane, the shell encapsulated NDs do not adsorb nonspecifically and they penetrate inside the cells. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Fluorenyl benzothiadiazole and benzoselenadiazole near-IR fluorescent probes for two-photon fluorescence imaging (Conference Presentation)

    Science.gov (United States)

    Belfield, Kevin D.; Yao, Sheng; Kim, Bosung; Yue, Xiling

    2016-03-01

    Imaging biological samples with two-photon fluorescence (2PF) microscopy has the unique advantage of resulting high contrast 3D resolution subcellular image that can reach up to several millimeters depth. 2PF probes that absorb and emit at near IR region need to be developed. Two-photon excitation (2PE) wavelengths are less concerned as 2PE uses wavelengths doubles the absorption wavelength of the probe, which means 2PE wavelengths for probes even with absorption at visible wavelength will fall into NIR region. Therefore, probes that fluoresce at near IR region with high quantum yields are needed. A series of dyes based on 5-thienyl-2, 1, 3-benzothiadiazole and 5-thienyl-2, 1, 3-benzoselenadiazole core were synthesized as near infrared two-photon fluorophores. Fluorescence maxima wavelengths as long as 714 nm and fluorescence quantum yields as high as 0.67 were achieved. The fluorescence quantum yields of the dyes were nearly constant, regardless of solvents polarity. These diazoles exhibited large Stokes shift (GM), and high two-photon fluorescence figure of merit (FM , 1.04×10-2 GM). Cells incubated on a 3D scaffold with one of the new probes (encapsulated in Pluronic micelles) exhibited bright fluorescence, enabling 3D two-photon fluorescence imaging to a depth of 100 µm.

  12. Development of a fluorescent microscope combined with a real-time autoradiography system

    International Nuclear Information System (INIS)

    Rai, Hiroki; Kanno, Satomi; Hayashi, Yoshitake; Nihei, Naoto; Nakanishi, Tomoko M.

    2008-01-01

    For combination with microscope, we developed real-time autoradiography system for micro-scale analysis with adjustment of the CsI(Ti) scintillator thickness for higher resolution and applying tapered fiber optic plate for magnification of autoradiograph image. We combined real-time autoradiography system with an inverted fluorescent microscope so that an autoradiograph image as well as fluorescent image, bright-field image can be acquired at the same time. In the case of observation of sliced soybean stalk traced 45 CaCl, the fluorescent and bright-field image was acquired which magnified to 50 times, the autoradiograph image of 45 Ca distribution in the tissue was acquired in almost same scale. The new microscopic system which can acquire autoradiograph image of labeled signals (low molecular weight) is expected to develop the signal transduction study and gene expression, combined with fluorescent protein techniques such as GFP etc. (author)

  13. Reviews in fluorescence 2008

    CERN Document Server

    Geddes, Chris D

    2010-01-01

    This volume serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence spectroscopy. It summarizes the year's progress in fluorescence and its applications as well as includes authoritative analytical reviews.

  14. Fluorescent optical position sensor

    Science.gov (United States)

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  15. Dark Skies, Bright Kids! Year 3

    Science.gov (United States)

    Whelan, David G.; Johnson, K. E.; Barcos-Munoz, L. D.; Beaton, R. L.; Borish, J.; Corby, J. F.; Dorsey, G.; Gugliucci, N. E.; Prager, B. J.; Ries, P. A.; Romero, C. E.; Sokal, K. R.; Tang, X.; Walker, L. M.; Yang, A. J.; Zasowski, G.

    2012-01-01

    Dark Skies, Bright Kids! (DSBK) is a program that brings astronomy education to elementary schools throughout central Virginia. In a relaxed, out-of-classroom atmosphere, we are able to foster the innate curiosity that young students have about science and the world around them. We target schools that are under-served due to their rural locale or special needs students, demonstrating that science is a fun and creative process to a segment of the population that might not otherwise be exposed to astronomy. Families are included in the learning experience during semi-annual `star parties'. Since last January, we have expanded the breadth and depth of our educational capabilities. We have developed new programs for use in our digital planetarium. We held the first Central Virginia Star Party, providing an atmosphere where local children from multiple schools were able to share their love for astronomy. Local government and University officials were also invited so that they could experience our focused science outreach. Most recently, we have become part of Ivy Creek School's Club Day activities, bringing our program to a new segment of the elementary school system in Albemarle County: those that have `low-incidence' disabilities, requiring special attention. We continue to develop a curriculum for after-school programs that functions as either a series of one-time activities or several months of focused outreach at one school. Many of these activities are provided on our website, http://www.astro.virginia.edu/dsbk/, for the wider astronomical community, including the new planetarium work. We have extended our book project to include two bilingual astronomy books called `Snapshots of the Universe,' one in Spanish and English, the other in French and English. These books introduce young people to some of the many wonders of the Universe through art and captions developed by DSBK volunteers.

  16. Bright field electron microscopy of biological specimens

    International Nuclear Information System (INIS)

    Johansen, B.V.

    1976-01-01

    A preirradiation procedure is described which preserves negatively stained morphological features in bright field electron micrographs to a resolution of about 1.2 nm. Prior to microscopy the pre-irradiation dose (1.6 x 10 -3 C cm -2 ) is given at low electron optical magnification at five different areas on the grid (the centre plus four 'corners'). This pre-irradiation can be measured either with a Faraday cage or through controlled exposure-developing conditions. Uranyl formate stained T2 bacteriophages and stacked disk aggregates of Tobacco Mosaic Virus (TMV) protein served as test objects. A comparative study was performed on specimens using either the pre-irradiation procedure or direct irradiation by the 'minimum beam exposure' technique. Changes in the electron diffraction pattern of the stain-protein complex and the disappearance of certain morphological features in the specimens were both used in order to compare the pre-irradiation method with the direct exposure technique. After identical electron exposures the pre-irradiation approach gave a far better preservation of specimen morphology. Consequently this procedure gives the microscopist more time to select and focus appropriate areas for imaging before deteriorations take place. The investigation also suggested that microscopy should be carried out between 60,000 and 100,000 times magnification. Within this magnification range, it is possible to take advantage of the phase contrast transfer characteristics of the objective lens while the electron load on the object is kept at a moderate level. Using the pre-irradiation procedure special features of the T2 bacteriophage morphology could be established. (author)

  17. Dark Skies, Bright Kids! Year 4

    Science.gov (United States)

    Sokal, Kimberly R.; Johnson, K. E.; Barcos-Munoz, L. D.; Beaton, R.; Borish, J.; Crawford, S. B.; Corby, J.; Damke, G.; Dean, J.; Dorsey, G.; Jackson, L.; Liss, S.; Oza, A.; Peacock, S.; Prager, B.; Romero, C.; Sivakoff, G. R.; Walker, L.; Whelan, D. G.; Zucker, C.

    2013-01-01

    Aiming to engage young children's natural excitement and curiosity, the outreach group Dark Skies, Bright Kids (DSBK) brings a hands-on approach to astronomy to elementary schools in Virginia. We hope to enhance children's view and understanding of science while exploring the Universe using fun activities. DSBK focuses on rural and underserved schools in Albemarle County and offers a semester-long astronomy club for third through fifth grade students. We believe regular interactions foster personal relationships between students and volunteers that encourage a life-long interest in science. In our fourth year of hosting clubs, we returned to Ivy Creek Elementary School, where we saw wonderful responses from a special group of students with `low-incidence' disabilities. DSBK has grown to realize a broader reach beyond local astronomy clubs; we hope to ignite a spark of interest in astronomy and science more widely- in more children, their families, and their teachers. We also hosted the Second Annual Central Virginia Star Party with an open invitation to the community to encourage families to enjoy astronomy together. Throughout the year, DSBK now holds 'one-off' programs (akin to astronomy field days) for elementary schools and children's groups throughout Virginia. Furthermore, we are in the final stages of a project to create two bilingual astronomy books called "Snapshots of the Universe", in Spanish and French with English translations. This art book will be made available online and we are working to get a copy in every elementary school in the state. DSBK has begun to reach out to elementary school teachers in order to provide them with useful and engaging classroom material. We have adapted our volunteer-created activities into useful and ready-to-use lessons, available online. After improvements based on research through interactions and feedback from teachers, we have explicitly identified the learning goals in terms of Virginia's Standards of Learning

  18. Safe biodegradable fluorescent particles

    Science.gov (United States)

    Martin, Sue I [Berkeley, CA; Fergenson, David P [Alamo, CA; Srivastava, Abneesh [Santa Clara, CA; Bogan, Michael J [Dublin, CA; Riot, Vincent J [Oakland, CA; Frank, Matthias [Oakland, CA

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  19. NIGHT SKY BRIGHTNESS ABOVE ZAGREB 2012.-2017.

    Directory of Open Access Journals (Sweden)

    Željko Andreić

    2018-01-01

    Full Text Available The night sky brightness at the RGN site (near the centre of Zagreb, Croatia was monitored form January 2012. to December 2017. The gathered data show that the average night sky brightness in this period did not change significantly, apart from differences caused by yearly variations in meteorological parameters. The nightly minima, maxima and mean values of the sky brightness do change considerably due to changes in meteorological conditions, often being between 2 and 3 magnitudes. The seasonal probability curves and histograms are constructed and are used to obtain additional information on the light pollution at the RGN site. They reveal that the night sky brightness clutters around two peaks, at about 15.0 mag/arcsec2 and at about 18.2 mag/arcsec2. The tendency to slightly lower brightness values in spring and summer can also be seen in the data. Two peaks correspond to cloudy and clear nights respectively, the difference in brightness between them being about 3 magnitudes. A crude clear/cloudy criterion can be defined too: the minimum between two peaks is around 16.7 mag/arcsec2. The brightness values smaller than thisare attributed to clear nights and vice-versa. Comparison with Vienna and Hong-Kong indicates that the light pollution of Zagreb is a few times larger.

  20. Optimization of fluorescent proteins

    NARCIS (Netherlands)

    Bindels, D.S.; Goedhart, J.; Hink, M.A.; van Weeren, L.; Joosen, L.; Gadella (jr.), T.W.J.; Engelborghs, Y.; Visser, A.J.W.G.

    2014-01-01

    Nowadays, fluorescent protein (FP) variants have been engineered to fluoresce in all different colors; to display photoswitchable, or photochromic, behavior; or to show yet other beneficial properties that enable or enhance a still growing set of new fluorescence spectroscopy and microcopy

  1. Gd(iii)-doped carbon dots as a dual fluorescent-MRI probe

    KAUST Repository

    Bourlinos, Athanasios B.; Bakandritsos, Aristides; Kouloumpis, Antonios; Gournis, Dimitrios; Krysmann, Marta; Giannelis, Emmanuel P.; Polakova, Katerina; Safarova, Klara; Hola, Katerina; Zboril, Radek

    2012-01-01

    We describe the synthesis of Gd(iii)-doped carbon dots as dual fluorescence-MRI probes for biomedical applications. The derived Gd(iii)-doped carbon dots show uniform particle size (3-4 nm) and gadolinium distribution and form stable dispersions in water. More importantly, they exhibit bright fluorescence, strong T1-weighted MRI contrast and low cytotoxicity. © The Royal Society of Chemistry 2012.

  2. Quadrature measurements of a bright squeezed state via sideband swapping

    DEFF Research Database (Denmark)

    Schneider, J.; Glockl, O.; Leuchs, G.

    2009-01-01

    The measurement of an arbitrary quadrature of a bright quantum state of light is a commonly requested action in many quantum information protocols, but it is experimentally challenging with previously proposed schemes. We suggest that the quadrature be measured at a specific sideband frequency...... of a bright quantum state by transferring the sideband modes under interrogation to a vacuum state and subsequently measuring the quadrature via homodyne detection. The scheme is implemented experimentally, and it is successfully tested with a bright squeezed state of light....

  3. Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system.

    Science.gov (United States)

    Heppert, Jennifer K; Dickinson, Daniel J; Pani, Ariel M; Higgins, Christopher D; Steward, Annette; Ahringer, Julie; Kuhn, Jeffrey R; Goldstein, Bob

    2016-11-07

    Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. These advances expand the potential of in vivo imaging experiments and facilitate experimentation with new, bright, photostable fluorescent proteins. Most quantitative comparisons of the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that govern their performance in cells or organisms. To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. We found that mNeonGreen was not as bright in vivo as predicted based on in vitro data but is a better tag than GFP for specific kinds of experiments, and we report on optimal red fluorescent proteins. These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos and suggest good candidate fluorescent proteins to test in other animal model systems for in vivo imaging experiments. © 2016 Heppert et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  4. Synergistic Combination of Unquenching and Plasmonic Fluorescence Enhancement in Fluorogenic Nucleic Acid Hybridization Probes.

    Science.gov (United States)

    Vietz, Carolin; Lalkens, Birka; Acuna, Guillermo P; Tinnefeld, Philip

    2017-10-11

    Fluorogenic nucleic acid hybridization probes are widely used for detecting and quantifying nucleic acids. The achieved sensitivity strongly depends on the contrast between a quenched closed form and an unquenched opened form with liberated fluorescence. So far, this contrast was improved by improving the quenching efficiency of the closed form. In this study, we modularly combine these probes with optical antennas used for plasmonic fluorescence enhancement and study the effect of the nanophotonic structure on the fluorescence of the quenched and the opened form. As quenched fluorescent dyes are usually enhanced more by fluorescence enhancement, a detrimental reduction of the contrast between closed and opened form was anticipated. In contrast, we could achieve a surprising increase of the contrast with full additivity of quenching of the dark form and fluorescence enhancement of the bright form. Using single-molecule experiments, we demonstrate that the additivity of the two mechanisms depends on the perfect quenching in the quenched form, and we delineate the rules for new nucleic acid probes for enhanced contrast and absolute brightness. Fluorogenic hybridization probes optimized not only for quenching but also for the brightness of the open form might find application in nucleic acid assays with PCR avoiding detection schemes.

  5. Near infrared fluorescent biliproteins generated from bacteriophytochrome AphB of Nostoc sp. PCC 7120.

    Science.gov (United States)

    Yuan, Che; Li, Hui-Zhen; Tang, Kun; Gärtner, Wolfgang; Scheer, Hugo; Zhou, Ming; Zhao, Kai-Hong

    2016-04-01

    The genome of the cyanobacterium Nostoc sp. PCC 7120 encodes a large number of putative bacteriophytochrome and cyanobacteriochrome photoreceptors that, due to their long-wavelength absorption and fluorescence emission, might serve as fluorescent tags in intracellular investigations. We show that the PAS-GAF domain of the bacteriophytochrome, AphB, binds biliverdin covalently and exhibits, besides its reversible photochemistry, a moderate fluorescence in the near infrared (NIR) spectral region. It was selected for further increasing the brightness while retaining the NIR fluorescence. In the first step, amino acids assumed to improve fluorescence were selectively mutated. The resulting variants were then subjected to several rounds of random mutagenesis and screened for enhanced fluorescence in the NIR. The brightness of optimized PAS-GAF variants increased more than threefold compared to that of wt AphB(1-321), with only insignificant spectral shifts (Amax around 695 nm, and Fmax around 720 nm). In general, the brightness increases with decreasing wavelengths, which allows for a selection of the fluorophore depending on the optical properties of the tissue. A spectral heterogeneity was observed when residue His260, located in close proximity to the chromophore, was mutated to Tyr, emphasizing the strong effects of the environment on the electronic properties of the bound biliverdin chromophore.

  6. Identifying Bright X-Ray Beasts

    Science.gov (United States)

    Kohler, Susanna

    2017-10-01

    Ultraluminous X-ray sources (ULXs) are astronomical sources of X-rays that, while dimmer than active galactic nuclei, are nonetheless brighter than any known stellar process. What are these beasts and why do they shine so brightly?Exceeding the LimitFirst discovered in the 1980s, ULXs are rare sources that have nonetheless been found in all types of galaxies. Though the bright X-ray radiation seems likely to be coming from compact objects accreting gas, theres a problem with this theory: ULXs outshine the Eddington luminosity for stellar-mass compact objects. This means that a stellar-mass object couldnt emit this much radiation isotropically without blowing itself apart.There are two alternative explanations commonly proposed for ULXs:Rather than being accreting stellar-mass compact objects, they are accreting intermediate-mass black holes. A hypothetical black hole of 100 solar masses or more would have a much higher Eddington luminosity than a stellar-mass black hole, making the luminosities that we observe from ULXs feasible.An example of one of the common routes the authors find for a binary system to become a ULX. In this case, the binary begins as two main sequence stars. As one star evolves off the main sequence, the binary undergoes a common envelope phase and a stage of mass transfer. The star ends its life as a supernova, and the resulting neutron star then accretes matter from the main sequence star as a ULX. [Wiktorowicz et al. 2017]They are ordinary X-ray binaries (a stellar-mass compact object accreting matter from a companion star), but they are undergoing a short phase of extreme accretion. During this time, their emission is beamed into jets, making them appear brighter than the Eddington luminosity.Clues from a New DiscoveryA few years ago, a new discovery shed some light on ULXs: M82 X-2, a pulsing ULX. Two more pulsing ULXs have been discovered since then, demonstrating that at least some ULXs contain pulsars i.e., neutron stars as the

  7. A new perspective on the infrared brightness temperature ...

    Indian Academy of Sciences (India)

    CEAWMT), ... temperatures clearly discriminates the cloud pixels of deep convective and ... utilized in the modelling of the histogram of infrared brightness temperature of deep convective and ..... Henderson-Sellers A 1978 Surface type and its effect.

  8. Operational Bright-Band Snow Level Detection Using Doppler Radar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A method to detect the bright-band snow level from radar reflectivity and Doppler vertical velocity data collection with an atmospheric profiling Doppler radar. The...

  9. Visible Color and Photometry of Bright Materials on Vesta

    Science.gov (United States)

    Schroder, S. E.; Li, J. Y.; Mittlefehldt, D. W.; Pieters, C. M.; De Sanctis, M. C.; Hiesinger, H.; Blewett, D. T.; Russell, C. T.; Raymond, C. A.; Keller, H. U.

    2012-01-01

    The Dawn Framing Camera (FC) collected images of the surface of Vesta at a pixel scale of 70 m in the High Altitude Mapping Orbit (HAMO) phase through its clear and seven color filters spanning from 430 nm to 980 nm. The surface of Vesta displays a large diversity in its brightness and colors, evidently related to the diverse geology [1] and mineralogy [2]. Here we report a detailed investigation of the visible colors and photometric properties of the apparently bright materials on Vesta in order to study their origin. The global distribution and the spectroscopy of bright materials are discussed in companion papers [3, 4], and the synthesis results about the origin of Vestan bright materials are reported in [5].

  10. Nimbus-5 ESMR Polar Gridded Brightness Temperatures, Version 2

    Data.gov (United States)

    National Aeronautics and Space Administration — The Nimbus-5 Electrically Scanning Microwave Radiometer (ESMR) data set consists of gridded brightness temperature arrays for the Arctic and Antarctic, spanning 11...

  11. Determination of Equilibrium Constant and Relative Brightness in FRET-FCS by Including the Third-Order Correlations.

    Science.gov (United States)

    Meng, Lingyi; He, Shanshan; Zhao, Xin Sheng

    2017-12-21

    Fluorescence correlation spectroscopy (FCS) encodes the information on the equilibrium constant (K), the relative fluorescence brightness of fluorophore (Q), and the forward and backward reaction rate constants (k + and k - ) on a physical or chemical relaxation. However, it has been a long-standing problem to completely resolve the FCS data to get the thermodynamic and kinetic information. Recently, we have solved the problem for fluorescence autocorrelation spectroscopy (FACS). Here, we extend the method to fluorescence cross-correlation spectroscopy (FCCS), which appears when FCS is coupled with fluorescence resonance energy transfer (FRET). Among 12 total second-order and third-order pre-exponential factors in a relaxation process probed by the FRET-FCS technique, 3 are independent. We presented and discussed 3 sets of explicit solutions to use these pre-exponential factors to calculate K and Q. Together with the relaxation time, the acquired K will allow people to obtain k + and k - , so that the goal of deciphering the FRET-FCS data will be fully reached. The theory is verified by extensive computer simulations and tested experimentally on a system of oligonucleotide hybridization.

  12. Analytically derived conversion of spectral band radiance to brightness temperature

    Energy Technology Data Exchange (ETDEWEB)

    Berk, Alexander [Spectral Sciences, Inc., 44th Avenue, Burlington, MA 01803 (United States)], E-mail: lex@spectral.com

    2008-05-15

    Simple analytic expressions for brightness temperature have been derived in terms of band response function spectral moments. Accuracy measures are also derived. Application of these formulas to GOES-12 Sounder thermal infrared bands produces brightness temperature residuals between -5.0 and 2.5 mK for a 150-400 K temperature range. The magnitude of residuals for the five ASTER Radiometer thermal infrared bands over the same temperature range is less than 0.22 mK.

  13. Retinal venous blood carbon monoxide response to bright light in male pigs: A preliminary study.

    Science.gov (United States)

    Oren, Dan A; Duda, Magdalena; Kozioł, Katarzyna; Romerowicz-Misielak, Maria; Koziorowska, Anna; Sołek, Przemysław; Nowak, Sławomir; Kulpa, Magdalena; Koziorowski, Marek

    2017-03-01

    The physical mechanism by which light is absorbed in the eye and has antidepressant and energizing effects in Seasonal Affective Disorder and other forms of psychiatric major depression is of scientific interest. This study was designed to explore one specific aspect of a proposed humoral phototransduction mechanism, namely that carbon monoxide (CO) levels increase in retinal venous blood in response to bright light. Eleven mature male pigs approximately six months of age were kept for 7days in darkness and fasted for 12h prior to surgery. Following mild sedation, anesthesia was induced. Silastic catheters were inserted into the dorsal nasal vein through the angular vein of the eye to reach the ophthalmic sinus, from which venous blood outflowing from the eye area was collected. The animals were exposed to 5000lx of fluorescent-generated white light. CO levels in the blood were analyzed by gas chromatography before and after 80min of light exposure. At baseline, mean CO levels in the retinal venous blood were 0.43±0.05(SE)nmol/ml. After bright light, mean CO levels increased to 0.54±0.06nmol/ml (two-tailed t-test plight exposure raises carbon monoxide levels in ophthalmic venous blood. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Bright-field Nanoscopy: Visualizing Nano-structures with Localized Optical Contrast Using a Conventional Microscope.

    Science.gov (United States)

    Suran, Swathi; Bharadwaj, Krishna; Raghavan, Srinivasan; Varma, Manoj M

    2016-04-26

    Most methods for optical visualization beyond the diffraction limit rely on fluorescence emission by molecular tags. Here, we report a method for visualization of nanostructures down to a few nanometers using a conventional bright-field microscope without requiring additional molecular tags such as fluorophores. The technique, Bright-field Nanoscopy, is based on the strong thickness dependent color of ultra-thin germanium on an optically thick gold film. We demonstrate the visualization of grain boundaries in chemical vapour deposited single layer graphene and the detection of single 40 nm Ag nanoparticles. We estimate a size detection limit of about 2 nm using this technique. In addition to visualizing nano-structures, this technique can be used to probe fluid phenomena at the nanoscale, such as transport through 2D membranes. We estimated the water transport rate through a 1 nm thick polymer film using this technique, as an illustration. Further, the technique can also be extended to study the transport of specific ions in the solution. It is anticipated that this technique will find use in applications ranging from single-nanoparticles resolved sensing to studying nanoscale fluid-solid interface phenomena.

  15. The effect of bright lines in environmental risk communication

    International Nuclear Information System (INIS)

    Wilson, K.N.; Desvousges, W.H.; Smith, K.V.; Payne, J.

    1993-01-01

    Bright lines in environmental risk communication refer to the specific levels at which an environmental risk becomes a serious health threat and action should be taken to mitigate its effects. This study examined the effect of ''bright lines'' in risk communication by emphasizing the radon exposure threshold level of 4 picocuries per liter. Specifically, the authors developed a computer-assisted interview containing bright-line versions of risk information. The bright-line version contained a range of possible radon levels, the corresponding number of estimated lung cancer cases, the relative health risk from radon compared to other health risks, and the EPA guidelines for mitigating levels above 4 picocuries in the home. The non-bright line version was identical to the bright-line version, except it did not include the EPA's mitigation recommendations. Effect measures included respondents' change in perceived risk after reading their materials, intended testing behavior, and advice to their neighbor for a specified radon level either above or below the 4 picocury threshold level. This paper discusses broader policy implications for designing effective risk communication programs

  16. Quantitative comparison of two particle tracking methods in fluorescence microscopy images

    CSIR Research Space (South Africa)

    Mabaso, M

    2013-09-01

    Full Text Available that cannot be analysed efficiently by means of manual analysis. In this study we compare the performance of two computer-based tracking methods for tracking of bright particles in fluorescence microscopy image sequences. The methods under comparison are...

  17. Cell motility dynamics: a novel segmentation algorithm to quantify multi-cellular bright field microscopy images.

    Directory of Open Access Journals (Sweden)

    Assaf Zaritsky

    Full Text Available Confocal microscopy analysis of fluorescence and morphology is becoming the standard tool in cell biology and molecular imaging. Accurate quantification algorithms are required to enhance the understanding of different biological phenomena. We present a novel approach based on image-segmentation of multi-cellular regions in bright field images demonstrating enhanced quantitative analyses and better understanding of cell motility. We present MultiCellSeg, a segmentation algorithm to separate between multi-cellular and background regions for bright field images, which is based on classification of local patches within an image: a cascade of Support Vector Machines (SVMs is applied using basic image features. Post processing includes additional classification and graph-cut segmentation to reclassify erroneous regions and refine the segmentation. This approach leads to a parameter-free and robust algorithm. Comparison to an alternative algorithm on wound healing assay images demonstrates its superiority. The proposed approach was used to evaluate common cell migration models such as wound healing and scatter assay. It was applied to quantify the acceleration effect of Hepatocyte growth factor/scatter factor (HGF/SF on healing rate in a time lapse confocal microscopy wound healing assay and demonstrated that the healing rate is linear in both treated and untreated cells, and that HGF/SF accelerates the healing rate by approximately two-fold. A novel fully automated, accurate, zero-parameters method to classify and score scatter-assay images was developed and demonstrated that multi-cellular texture is an excellent descriptor to measure HGF/SF-induced cell scattering. We show that exploitation of textural information from differential interference contrast (DIC images on the multi-cellular level can prove beneficial for the analyses of wound healing and scatter assays. The proposed approach is generic and can be used alone or alongside traditional

  18. Cell motility dynamics: a novel segmentation algorithm to quantify multi-cellular bright field microscopy images.

    Science.gov (United States)

    Zaritsky, Assaf; Natan, Sari; Horev, Judith; Hecht, Inbal; Wolf, Lior; Ben-Jacob, Eshel; Tsarfaty, Ilan

    2011-01-01

    Confocal microscopy analysis of fluorescence and morphology is becoming the standard tool in cell biology and molecular imaging. Accurate quantification algorithms are required to enhance the understanding of different biological phenomena. We present a novel approach based on image-segmentation of multi-cellular regions in bright field images demonstrating enhanced quantitative analyses and better understanding of cell motility. We present MultiCellSeg, a segmentation algorithm to separate between multi-cellular and background regions for bright field images, which is based on classification of local patches within an image: a cascade of Support Vector Machines (SVMs) is applied using basic image features. Post processing includes additional classification and graph-cut segmentation to reclassify erroneous regions and refine the segmentation. This approach leads to a parameter-free and robust algorithm. Comparison to an alternative algorithm on wound healing assay images demonstrates its superiority. The proposed approach was used to evaluate common cell migration models such as wound healing and scatter assay. It was applied to quantify the acceleration effect of Hepatocyte growth factor/scatter factor (HGF/SF) on healing rate in a time lapse confocal microscopy wound healing assay and demonstrated that the healing rate is linear in both treated and untreated cells, and that HGF/SF accelerates the healing rate by approximately two-fold. A novel fully automated, accurate, zero-parameters method to classify and score scatter-assay images was developed and demonstrated that multi-cellular texture is an excellent descriptor to measure HGF/SF-induced cell scattering. We show that exploitation of textural information from differential interference contrast (DIC) images on the multi-cellular level can prove beneficial for the analyses of wound healing and scatter assays. The proposed approach is generic and can be used alone or alongside traditional fluorescence single

  19. Fluorescent lighting with aluminum nitride phosphors

    Science.gov (United States)

    Cherepy, Nerine J.; Payne, Stephen A.; Seeley, Zachary M.; Srivastava, Alok M.

    2016-05-10

    A fluorescent lamp includes a glass envelope; at least two electrodes connected to the glass envelope; mercury vapor and an inert gas within the glass envelope; and a phosphor within the glass envelope, wherein the phosphor blend includes aluminum nitride. The phosphor may be a wurtzite (hexagonal) crystalline structure Al.sub.(1-x)M.sub.xN phosphor, where M may be drawn from beryllium, magnesium, calcium, strontium, barium, zinc, scandium, yttrium, lanthanum, cerium, praseodymium, europium, gadolinium, terbium, ytterbium, bismuth, manganese, silicon, germanium, tin, boron, or gallium is synthesized to include dopants to control its luminescence under ultraviolet excitation. The disclosed Al.sub.(1-x)M.sub.xN:Mn phosphor provides bright orange-red emission, comparable in efficiency and spectrum to that of the standard orange-red phosphor used in fluorescent lighting, Y.sub.2O.sub.3:Eu. Furthermore, it offers excellent lumen maintenance in a fluorescent lamp, and does not utilize "critical rare earths," minimizing sensitivity to fluctuating market prices for the rare earth elements.

  20. Fluorescence microscopy for the characterization of structural integrity

    Science.gov (United States)

    Street, Kenneth W.; Leonhardt, Todd A.

    1991-01-01

    The absorption characteristics of light and the optical technique of fluorescence microscopy for enhancing metallographic interpretation are presented. Characterization of thermally sprayed coatings by optical microscopy suffers because of the tendency for misidentification of the microstructure produced by metallographic preparation. Gray scale, in bright field microscopy, is frequently the only means of differentiating the actual structural details of porosity, cracking, and debonding of coatings. Fluorescence microscopy is a technique that helps to distinguish the artifacts of metallographic preparation (pullout, cracking, debonding) from the microstructure of the specimen by color contrasting structural differences. Alternative instrumentation and the use of other dye systems are also discussed. The combination of epoxy vacuum infiltration with fluorescence microscopy to verify microstructural defects is an effective means to characterize advanced materials and to assess structural integrity.

  1. Microwave heating of arginine yields highly fluorescent nanoparticles

    International Nuclear Information System (INIS)

    Philippidis, Aggelos; Stefanakis, Dimitrios; Anglos, Demetrios; Ghanotakis, Demetrios

    2013-01-01

    Brightly fluorescent nanoparticles were produced via a single-step, single-precursor procedure based on microwave heating of an aqueous solution of the amino acid arginine. Key structural and optical properties of the resulting Arg nanoparticles, Arg-dots, are reported and discussed with emphasis on the pH dependence of their fluorescence emission. The surface of the Arg-dots was functionalised through coupling to folic acid, opening up ways for connecting fluorescent nanoparticles to cancer cells. The generality and versatility of the microwave heating procedure was further demonstrated by the synthesis of different types of carbon nanoparticles, such as CE-dots, that were produced by use of citric acid and ethanolamine as precursors and compared to the Arg-dots.

  2. Quantum dots versus organic fluorophores in fluorescent deep-tissue imaging--merits and demerits.

    Science.gov (United States)

    Bakalova, Rumiana; Zhelev, Zhivko; Gadjeva, Veselina

    2008-12-01

    The use of fluorescence in deep-tissue imaging is rapidly expanding in last several years. The progress in fluorescent molecular probes and fluorescent imaging techniques gives an opportunity to detect single cells and even molecular targets in live organisms. The highly sensitive and high-speed fluorescent molecular sensors and detection devices allow the application of fluorescence in functional imaging. With the development of novel bright fluorophores based on nanotechnologies and 3D fluorescence scanners with high spatial and temporal resolution, the fluorescent imaging has a potential to become an alternative of the other non-invasive imaging techniques as magnetic resonance imaging, positron-emission tomography, X-ray, computing tomography. The fluorescent imaging has also a potential to give a real map of human anatomy and physiology. The current review outlines the advantages of fluorescent nanoparticles over conventional organic dyes in deep-tissue imaging in vivo and defines the major requirements to the "perfect fluorophore". The analysis proceeds from the basic principles of fluorescence and major characteristics of fluorophores, light-tissue interactions, and major limitations of fluorescent deep-tissue imaging. The article is addressed to a broad readership - from specialists in this field to university students.

  3. Color and emotion: effects of hue, saturation, and brightness.

    Science.gov (United States)

    Wilms, Lisa; Oberfeld, Daniel

    2017-06-13

    Previous studies on emotional effects of color often failed to control all the three perceptual dimensions of color: hue, saturation, and brightness. Here, we presented a three-dimensional space of chromatic colors by independently varying hue (blue, green, red), saturation (low, medium, high), and brightness (dark, medium, bright) in a factorial design. The 27 chromatic colors, plus 3 brightness-matched achromatic colors, were presented via an LED display. Participants (N = 62) viewed each color for 30 s and then rated their current emotional state (valence and arousal). Skin conductance and heart rate were measured continuously. The emotion ratings showed that saturated and bright colors were associated with higher arousal. The hue also had a significant effect on arousal, which increased from blue and green to red. The ratings of valence were the highest for saturated and bright colors, and also depended on the hue. Several interaction effects of the three color dimensions were observed for both arousal and valence. For instance, the valence ratings were higher for blue than for the remaining hues, but only for highly saturated colors. Saturated and bright colors caused significantly stronger skin conductance responses. Achromatic colors resulted in a short-term deceleration in the heart rate, while chromatic colors caused an acceleration. The results confirm that color stimuli have effects on the emotional state of the observer. These effects are not only determined by the hue of a color, as is often assumed, but by all the three color dimensions as well as their interactions.

  4. Beam brightness calculation for analytical and empirical distribution functions

    International Nuclear Information System (INIS)

    Myers, T.J.; Boulais, K.A.; O, Y.S.; Rhee, M.J.

    1992-01-01

    The beam brightness, a figure of merit for a beam quality useful for high-current low-emittance beams, was introduced by van Steenbergen as B = I/V 4 , where I is the beam current and V 4 is the hypervolume in the four-dimensional trace space occupied by the beam particles. Customarily, the brightness is expressed in terms of the product of emittances ε x ε y as B = ηI/(π 2 ε x ε y ), where η is a form factor of order unity which depends on the precise definition of emittance and hypervolume. Recently, a refined definition of the beam brightness based on the arithmetic mean value defined in statistics is proposed. The beam brightness is defined as B triple-bond 4 > = I -1 ∫ ρ 4 2 dxdydx'dy', where I is the beam current given by I ∫ ρ 4 dxdydx'dy'. Note that in this definition, neither the hypervolume V 4 nor the emittance, are explicitly used; the brightness is determined solely by the distribution function. Brightnesses are unambiguously calculated and expressed analytically in terms of the respective beam current and effective emittance for a few commonly used distribution functions, including Maxwellian and water-bag distributions. Other distributions of arbitrary shape frequently encountered in actual experiments are treated numerically. The resulting brightnesses are expressed in the form B = ηI/(π 2 ε x ε y ), and η is found to be weakly dependent on the form of velocity distribution as well as spatial distribution

  5. Atomic-fluorescence spectrophotometry

    International Nuclear Information System (INIS)

    Bakhturova, N.F.; Yudelevich, I.G.

    1975-01-01

    Atomic-fluorescence spectrophotometry, a comparatively new method for the analysis of trace quantities, has developed rapidly in the past ten years. Theoretical and experimental studies by many workers have shown that atomic-fluorescence spectrophotometry (AFS) is capable of achieving a better limit than atomic absorption for a large number of elements. The present review examines briefly the principles of atomic-fluorescence spectrophotometry and the types of fluorescent transition. The excitation sources, flame and nonflame atomizers, used in AFS are described. The limits of detection achieved up to the present, using flame and nonflame methods of atomization are given

  6. Fluorescence of irradiated hydrocarbons

    International Nuclear Information System (INIS)

    Gulis, I.G.; Evdokimenko, V.M.; Lapkovskij, M.P.; Petrov, P.T.; Gulis, I.M.; Markevich, S.V.

    1977-01-01

    A visible fluorescence has been found out in γ-irradiated aqueous of carbohydrates. Two bands have been distinguished in fluorescence spectra of the irradiated solution of dextran: a short-wave band lambdasub(max)=140 nm (where lambda is a wave length) at lambdasub(β)=380 nm and a long-wave band with lambdasub(max)=540 nm at lambdasub(β)=430 nm. A similar form of the spectrum has been obtained for irradiated solutions of starch, amylopectin, lowmolecular glucose. It has been concluded that a macromolecule of polysaccharides includes fluorescent centres. A relation between fluorescence and α-oxiketon groups formed under irradiation has been pointed out

  7. A green fluorescent protein with photoswitchable emission from the deep sea.

    Directory of Open Access Journals (Sweden)

    Alexander Vogt

    Full Text Available A colorful variety of fluorescent proteins (FPs from marine invertebrates are utilized as genetically encoded markers for live cell imaging. The increased demand for advanced imaging techniques drives a continuous search for FPs with new and improved properties. Many useful FPs have been isolated from species adapted to sun-flooded habitats such as tropical coral reefs. It has yet remained unknown if species expressing green fluorescent protein (GFP-like proteins also exist in the darkness of the deep sea. Using a submarine-based and -operated fluorescence detection system in the Gulf of Mexico, we discovered ceriantharians emitting bright green fluorescence in depths between 500 and 600 m and identified a GFP, named cerFP505, with bright fluorescence emission peaking at 505 nm. Spectroscopic studies showed that approximately 15% of the protein bulk feature reversible ON/OFF photoswitching that can be induced by alternating irradiation with blue und near-UV light. Despite being derived from an animal adapted to essentially complete darkness and low temperatures, cerFP505 maturation in living mammalian cells at 37 degrees C, its brightness and photostability are comparable to those of EGFP and cmFP512 from shallow water species. Therefore, our findings disclose the deep sea as a potential source of GFP-like molecular marker proteins.

  8. Fish with red fluorescent eyes forage more efficiently under dim, blue-green light conditions.

    Science.gov (United States)

    Harant, Ulrike Katharina; Michiels, Nicolaas Karel

    2017-04-20

    Natural red fluorescence is particularly conspicuous in the eyes of some small, benthic, predatory fishes. Fluorescence also increases in relative efficiency with increasing depth, which has generated speculation about its possible function as a "light organ" to detect cryptic organisms under bluish light. Here we investigate whether foraging success is improved under ambient conditions that make red fluorescence stand out more, using the triplefin Tripterygion delaisi as a model system. We repeatedly presented 10 copepods to individual fish (n = 40) kept under a narrow blue-green spectrum and compared their performance with that under a broad spectrum with the same overall brightness. The experiment was repeated for two levels of brightness, a shaded one representing 0.4% of the light present at the surface and a heavily shaded one with about 0.01% of the surface brightness. Fish were 7% more successful at catching copepods under the narrow, fluorescence-friendly spectrum than under the broad spectrum. However, this effect was significant under the heavily shaded light treatment only. This outcome corroborates previous predictions that fluorescence may be an adaptation to blue-green, heavily shaded environments, which coincides with the opportunistic biology of this species that lives in the transition zone between exposed and heavily shaded microhabitats.

  9. Brightness-normalized Partial Least Squares Regression for hyperspectral data

    International Nuclear Information System (INIS)

    Feilhauer, Hannes; Asner, Gregory P.; Martin, Roberta E.; Schmidtlein, Sebastian

    2010-01-01

    Developed in the field of chemometrics, Partial Least Squares Regression (PLSR) has become an established technique in vegetation remote sensing. PLSR was primarily designed for laboratory analysis of prepared material samples. Under field conditions in vegetation remote sensing, the performance of the technique may be negatively affected by differences in brightness due to amount and orientation of plant tissues in canopies or the observing conditions. To minimize these effects, we introduced brightness normalization to the PLSR approach and tested whether this modification improves the performance under changing canopy and observing conditions. This test was carried out using high-fidelity spectral data (400-2510 nm) to model observed leaf chemistry. The spectral data was combined with a canopy radiative transfer model to simulate effects of varying canopy structure and viewing geometry. Brightness normalization enhanced the performance of PLSR by dampening the effects of canopy shade, thus providing a significant improvement in predictions of leaf chemistry (up to 3.6% additional explained variance in validation) compared to conventional PLSR. Little improvement was made on effects due to variable leaf area index, while minor improvement (mostly not significant) was observed for effects of variable viewing geometry. In general, brightness normalization increased the stability of model fits and regression coefficients for all canopy scenarios. Brightness-normalized PLSR is thus a promising approach for application on airborne and space-based imaging spectrometer data.

  10. Sky brightness and twilight measurements at Jogyakarta city, Indonesia

    International Nuclear Information System (INIS)

    Herdiwijaya, Dhani

    2016-01-01

    The sky brightness measurements were performed using a portable photometer. A pocket-sized and low-cost photometer has 20 degree area measurement, and spectral ranges between 320-720 nm with output directly in magnitudes per arc second square (mass) unit. The sky brightness with 3 seconds temporal resolutions was recorded at Jogyakarta city (110° 25’ E; 70° 52’ S; elevation 100 m) within 136 days in years from 2014 to 2016. The darkest night could reach 22.61 mpass only in several seconds, with mean value 18.8±0.7 mpass and temperature variation 23.1±1.2 C. The difference of mean sky brightness between before and after midnight was about -0.76 mpass or 2.0 times brighter. Moreover, the sky brightness and temperature fluctuations were more stable in after midnight than in before midnight. It is suggested that city light pollution affects those variations, and subsequently duration of twilight. By comparing twilight brightness for several places, we also suggest a 17° solar dip or about 66 minutes before sunrise for new time of Fajr prayer. (paper)

  11. Eosin fluorescence: A diagnostic tool for quantification of liver injury.

    Science.gov (United States)

    Ali, Hamid; Ali, Safdar; Mazhar, Maryam; Ali, Amjad; Jahan, Azra; Ali, Abid

    2017-09-01

    Hepatitis is one of the most common life threatening diseases. The diagnosis is mainly based on biochemical analysis such as liver function test. However, histopathological evaluation of liver serves far better for more accurate final diagnosis. The goal of our study was to evaluate the eosin fluorescence pattern in CCl 4 -induced liver injury model compared with normal and different treatment groups. For this purpose, liver tissues were stained with H/E and examined under bright field microscope but the fluorescence microscopy of H/E stained slides provided an interesting fluorescence pattern and was quite helpful in identifying different structures. Interesting fluorescence patterns were obtained with FITC, Texas Red and Dual channel filter cubes that were quite helpful in identifying different morphological features of the liver. During the course of hepatic injury, liver cells undergo necrosis, apoptosis and overall cellular microenvironment is altered due to the modification of proteins and other intracellular molecules. Intensified eosin fluorescence was observed around the central vein of injured liver compared to normal indicating enhanced binding of eosin to the more exposed amino acid residues. To conclude, eosin fluorescence pattern varies with the health status of a tissue and can be used further for the diagnosis and quantification of severity of various liver diseases. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Brightness and uniformity measurements of plastic scintillator tiles at the CERN H2 test beam

    Science.gov (United States)

    Chatrchyan, S.; Sirunyan, A. M.; Tumasyan, A.; Litomin, A.; Mossolov, V.; Shumeiko, N.; Van De Klundert, M.; Van Haevermaet, H.; Van Mechelen, P.; Van Spilbeeck, A.; Alves, G. A.; Aldá Júnior, W. L.; Hensel, C.; Carvalho, W.; Chinellato, J.; De Oliveira Martins, C.; Matos Figueiredo, D.; Mora Herrera, C.; Nogima, H.; Prado Da Silva, W. L.; Tonelli Manganote, E. J.; Vilela Pereira, A.; Finger, M.; Finger, M., Jr.; Kveton, A.; Tomsa, J.; Adamov, G.; Tsamalaidze, Z.; Behrens, U.; Borras, K.; Campbell, A.; Costanza, F.; Gunnellini, P.; Lobanov, A.; Melzer-Pellmann, I.-A.; Muhl, C.; Roland, B.; Sahin, M.; Saxena, P.; Hegde, V.; Kothekar, K.; Pandey, S.; Sharma, S.; Beri, S. B.; Bhawandeep, B.; Chawla, R.; Kalsi, A.; Kaur, A.; Kaur, M.; Walia, G.; Bhattacharya, S.; Ghosh, S.; Nandan, S.; Purohit, A.; Sharan, M.; Banerjee, S.; Bhattacharya, S.; Chatterjee, S.; Das, P.; Guchait, M.; Jain, S.; Kumar, S.; Maity, M.; Majumder, G.; Mazumdar, K.; Patil, M.; Sarkar, T.; Juodagalvis, A.; Afanasiev, S.; Bunin, P.; Ershov, Y.; Golutvin, I.; Malakhov, A.; Moisenz, P.; Smirnov, V.; Zarubin, A.; Chadeeva, M.; Chistov, R.; Danilov, M.; Popova, E.; Rusinov, V.; Andreev, Yu.; Dermenev, A.; Karneyeu, A.; Krasnikov, N.; Tlisov, D.; Toropin, A.; Epshteyn, V.; Gavrilov, V.; Lychkovskaya, N.; Popov, V.; Pozdnyakov, I.; Safronov, G.; Toms, M.; Zhokin, A.; Baskakov, A.; Belyaev, A.; Boos, E.; Dubinin, M.; Dudko, L.; Ershov, A.; Gribushin, A.; Kaminskiy, A.; Klyukhin, V.; Kodolova, O.; Lokhtin, I.; Miagkov, I.; Obraztsov, S.; Petrushanko, S.; Savrin, V.; Snigirev, A.; Andreev, V.; Azarkin, M.; Dremin, I.; Kirakosyan, M.; Leonidov, A.; Terkulov, A.; Bitioukov, S.; Elumakhov, D.; Kalinin, A.; Krychkine, V.; Mandrik, P.; Petrov, V.; Ryutin, R.; Sobol, A.; Troshin, S.; Volkov, A.; Sekmen, S.; Medvedeva, T.; Rumerio, P.; Adiguzel, A.; Bakirci, N.; Boran, F.; Cerci, S.; Damarseckin, S.; Demiroglu, Z. S.; Dölek, F.; Dozen, C.; Dumanoglu, I.; Eskut, E.; Girgis, S.; Gokbulut, G.; Guler, Y.; Hos, I.; Kangal, E. E.; Kara, O.; Kayis Topaksu, A.; Işik, C.; Kiminsu, U.; Oglakci, M.; Onengut, G.; Ozdemir, K.; Ozturk, S.; Polatoz, A.; Sunar Cerci, D.; Tali, B.; Tok, U. G.; Topakli, H.; Turkcapar, S.; Zorbakir, I. S.; Zorbilmez, C.; Bilin, B.; Isildak, B.; Karapinar, G.; Murat Guler, A.; Ocalan, K.; Yalvac, M.; Zeyrek, M.; Atakisi, I. O.; Gülmez, E.; Kaya, M.; Kaya, O.; Koseyan, O. K.; Ozcelik, O.; Ozkorucuklu, S.; Tekten, S.; Yetkin, E. A.; Yetkin, T.; Cankocak, K.; Sen, S.; Boyarintsev, A.; Grynyov, B.; Levchuk, L.; Popov, V.; Sorokin, P.; Flacher, H.; Borzou, A.; Call, K.; Dittmann, J.; Hatakeyama, K.; Liu, H.; Pastika, N.; Buccilli, A.; Cooper, S. I.; Henderson, C.; West, C.; Arcaro, D.; Gastler, D.; Hazen, E.; Rohlf, J.; Sulak, L.; Wu, S.; Zou, D.; Hakala, J.; Heintz, U.; Kwok, K. H. M.; Laird, E.; Landsberg, G.; Mao, Z.; Yu, D. R.; Gary, J. W.; Ghiasi Shirazi, S. M.; Lacroix, F.; Long, O. R.; Wei, H.; Bhandari, R.; Heller, R.; Stuart, D.; Yoo, J. H.; Chen, Y.; Duarte, J.; Lawhorn, J. M.; Nguyen, T.; Spiropulu, M.; Winn, D.; Abdullin, S.; Apresyan, A.; Apyan, A.; Banerjee, S.; Chlebana, F.; Freeman, J.; Green, D.; Hare, D.; Hirschauer, J.; Joshi, U.; Lincoln, D.; Los, S.; Pedro, K.; Spalding, W. J.; Strobbe, N.; Tkaczyk, S.; Whitbeck, A.; Linn, S.; Markowitz, P.; Martinez, G.; Bertoldi, M.; Hagopian, S.; Hagopian, V.; Kolberg, T.; Baarmand, M. M.; Noonan, D.; Roy, T.; Yumiceva, F.; Bilki, B.; Clarida, W.; Debbins, P.; Dilsiz, K.; Durgut, S.; Gandrajula, R. P.; Haytmyradov, M.; Khristenko, V.; Merlo, J.-P.; Mermerkaya, H.; Mestvirishvili, A.; Miller, M.; Moeller, A.; Nachtman, J.; Ogul, H.; Onel, Y.; Ozok, F.; Penzo, A.; Schmidt, I.; Snyder, C.; Southwick, D.; Tiras, E.; Yi, K.; Al-bataineh, A.; Bowen, J.; Castle, J.; McBrayer, W.; Murray, M.; Wang, Q.; Kaadze, K.; Maravin, Y.; Mohammadi, A.; Saini, L. K.; Baden, A.; Belloni, A.; Calderon, J. D.; Eno, S. C.; Feng, Y. B.; Ferraioli, C.; Grassi, T.; Hadley, N. J.; Jeng, G.-Y.; Kellogg, R. G.; Kunkle, J.; Mignerey, A.; Ricci-Tam, F.; Shin, Y. H.; Skuja, A.; Yang, Z. S.; Yao, Y.; Brandt, S.; D'Alfonso, M.; Hu, M.; Klute, M.; Niu, X.; Chatterjee, R. M.; Evans, A.; Frahm, E.; Kubota, Y.; Lesko, Z.; Mans, J.; Ruckstuhl, N.; Heering, A.; Karmgard, D. J.; Musienko, Y.; Ruchti, R.; Wayne, M.; Benaglia, A. D.; Mei, K.; Tully, C.; Bodek, A.; de Barbaro, P.; Galanti, M.; Garcia-Bellido, A.; Khukhunaishvili, A.; Lo, K. H.; Vishnevskiy, D.; Zielinski, M.; Agapitos, A.; Amouzegar, M.; Chou, J. P.; Hughes, E.; Saka, H.; Sheffield, D.; Akchurin, N.; Damgov, J.; De Guio, F.; Dudero, P. R.; Faulkner, J.; Gurpinar, E.; Kunori, S.; Lamichhane, K.; Lee, S. W.; Libeiro, T.; Mengke, T.; Muthumuni, S.; Undleeb, S.; Volobouev, I.; Wang, Z.; Goadhouse, S.; Hirosky, R.; Wang, Y.

    2018-01-01

    We study the light output, light collection efficiency and signal timing of a variety of organic scintillators that are being considered for the upgrade of the hadronic calorimeter of the CMS detector. The experimental data are collected at the H2 test-beam area at CERN, using a 150 GeV muon beam. In particular, we investigate the usage of over-doped and green-emitting plastic scintillators, two solutions that have not been extensively considered. We present a study of the energy distribution in plastic-scintillator tiles, the hit efficiency as a function of the hit position, and a study of the signal timing for blue and green scintillators.

  13. Brightness and uniformity measurements of plastic scintillator tiles at the CERN H2 test beam

    Energy Technology Data Exchange (ETDEWEB)

    Chatrchyan, S.; et al.

    2017-09-25

    We study the light output, light collection efficiency and signal timing of a variety of organic scintillators that are being considered for the upgrade of the hadronic calorimeter of the CMS detector. The experimental data are collected at the H2 test-beam area at CERN, using a 150 GeV muon beam. In particular, we investigate the usage of over-doped and green-emitting plastic scintillator, two solutions that have not been extensively considered. We present a study of the energy distribution in plastic-scintillator tiles, the hit efficiency as a function of the hit position, and a study of the signal timing for blue and green scintillators.

  14. Membranes and Fluorescence microscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2009-01-01

    Fluorescence spectroscopy-based techniques using conventional fluorimeters have been extensively applied since the late 1960s to study different aspects of membrane-related phenomena, i.e., mainly relating to lipid-lipid and lipid-protein (peptide) interactions. Even though fluorescence...

  15. Multimodal fluorescence imaging spectroscopy

    NARCIS (Netherlands)

    Stopel, Martijn H W; Blum, Christian; Subramaniam, Vinod; Engelborghs, Yves; Visser, Anthonie J.W.G.

    2014-01-01

    Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample.

  16. Bright and Not-So-Bright Prospects for Women in Physics in China-Beijing

    Science.gov (United States)

    Wu, Ling-An; Yang, Zhongqin; Ma, Wanyun

    2009-04-01

    Science in China-Beijing is enjoying a healthy increase in funding year by year, so the prospects for physicists are also bright. However, employment discrimination against women, formerly unthinkable, is becoming more and more explicit as the country evolves toward a market economy. Some recruitment notices bluntly state that only men will be considered, or impose restrictions upon potential female candidates. Female associate professors in many institutions are forced to retire at age 55, compared with 60 for men. This double-pinching discrimination against both younger and older women threatens to lead to a "pincer" effect, more serious than the "scissors" effect. Indeed, the ratio of senior-level women physicists in general has dropped significantly in recent years in China. Ironically, the number of female students applying for graduate studies is on the rise, as it is becoming increasingly difficult for them to compete with men in the job market with just an undergraduate degree. The Chinese Physical Society has made certain efforts to promote the image of women physicists, but it will take time and effort to reverse the trend.

  17. Low dimensional neutron moderators for enhanced source brightness

    DEFF Research Database (Denmark)

    Mezei, Ferenc; Zanini, Luca; Takibayev, Alan

    2014-01-01

    In a recent numerical optimization study we have found that liquid para-hydrogen coupled cold neutron moderators deliver 3–5 times higher cold neutron brightness at a spallation neutron source if they take the form of a flat, quasi 2-dimensional disc, in contrast to the conventional more voluminous...... for cold neutrons. This model leads to the conclusions that the optimal shape for high brightness para-hydrogen neutron moderators is the quasi 1-dimensional tube and these low dimensional moderators can also deliver much enhanced cold neutron brightness in fission reactor neutron sources, compared...... to the much more voluminous liquid D2 or H2 moderators currently used. Neutronic simulation calculations confirm both of these theoretical conclusions....

  18. Bright Stuff on Ceres = Sulfates and Carbonates on CI Chondrites

    Science.gov (United States)

    Zolensky, Michael; Chan, Queenie H. S.; Gounelle, Matthieu; Fries, Marc

    2016-01-01

    Recent reports of the DAWN spacecraft's observations of the surface of Ceres indicate that there are bright areas, which can be explained by large amounts of the Mg sulfate hexahydrate (MgSO4•6(H2O)), although the identification appears tenuous. There are preliminary indications that water is being evolved from these bright areas, and some have inferred that these might be sites of contemporary hydro-volcanism. A heat source for such modern activity is not obvious, given the small size of Ceres, lack of any tidal forces from nearby giant planets, probable age and presumed bulk composition. We contend that observations of chondritic materials in the lab shed light on the nature of the bright spots on Ceres

  19. Low surface brightness galaxies in the cluster A1367

    International Nuclear Information System (INIS)

    Davies, J.I.; Phillipps, S.; Disney, M.J.

    1989-01-01

    We have obtained deep CCD frames of apparently blank regions of sky in the hope of detecting very low surface brightness (LSB) objects in the cluster A1367. We discuss our data reduction, and image detection and selection techniques. If the galaxies detected are actually cluster members then they are dwarfs and the conclusions of a previous paper on the Fornax cluster are essentially confirmed. One area of variance is that the lowest surface brightness galaxies do not appear to be preferentially concentrated towards the cluster centre. This can be explained by there being a much larger density of dwarf galaxies over this bright galaxy-rich region of the universe. We find over our small area approximately four times as many LSB galaxies as would be expected from our Fornax data. We speculate on the possible origin and likely intensity of intergalactic light within clusters. (author)

  20. Influence of Type of Electric Bright Light on the Attraction of the African Giant Water Bug, Lethocerus indicus (Hemiptera: Belostomatidae

    Directory of Open Access Journals (Sweden)

    Luke Chinaru Nwosu

    2012-01-01

    Full Text Available This study investigated the influence of type of electric bright light (produced by fluorescent light tube and incandescent light bulb on the attraction of the African giant water bug, Lethocerus indicus (Hemiptera: Belostomatidae. Four fluorescent light tubes of 15 watts each, producing white-coloured light and four incandescent light bulbs of 60 watts each, producing yellow-coloured light, but both producing the same amount of light, were varied and used for the experiments. Collections of bugs at experimental house were done at night between the hours of 8.30 pm and 12 mid-night on daily basis for a period of four months per experiment in the years 2008 and 2009. Lethocerus indicus whose presence in any environment has certain implications was the predominant belostomatid bug in the area. Use of incandescent light bulbs in 2009 significantly attracted more Lethocerus indicus 103 (74.6% than use of fluorescent light tubes 35 (25.41% in 2008 [4.92=0.0001]. However, bug’s attraction to light source was not found sex dependent [>0.05; (>0.18=0.4286 and >0.28=0.3897]. Therefore, this study recommends the use of fluorescent light by households, campgrounds, and other recreational centres that are potentially exposed to the nuisance of the giant water bugs. Otherwise, incandescent light bulbs should be used when it is desired to attract the presence of these aquatic bugs either for food or scientific studies.

  1. Leonids 2017 from Norway – A bright surprise!

    Science.gov (United States)

    Gaarder, K.

    2018-01-01

    I am very pleased to have been able to observe near maximum activity of the Leonids, and clearly witnessed the unequal mass distribution during these hours. A lot of bright Leonids were seen, followed by a short period of high activity of fainter meteors, before a sharp drop in activity. The Leonids is undoubtedly a shower to watch closely, with its many variations in activity level and magnitude distribution. I already look forward to observing the next years’ display, hopefully under a dark and clear sky, filled with bright meteors!

  2. Sky Brightness During Eclipses: A Compendium from the Literature

    Science.gov (United States)

    1974-08-05

    Star Sightings 1880A 11 Jan 1880 Santa- Lucia Jupiter -3 Mars .3 No stars 1882A 17 May 1882 Sohag, Egypt Stars shone brightly 1883A 6 M.»y 1883...1870 E 1880 A 1886 BA 1887 F 1889 A2 1900 EB 1914 A 1927 B Location London Venice Bue Island Gottenbui g Vumpurthy Seville Santa- Lucia ...manner, and at the peak of day the stars ap- peared brightly. (Tatti Annali sacri della citta di Como.) 5 MAY 840 840 DB Ginzel p. 668 Italy In

  3. Brightness distribution data on 2918 radio sources at 365 MHz

    International Nuclear Information System (INIS)

    Cotton, W.D.; Owen, F.N.; Ghigo, F.D.

    1975-01-01

    This paper is the second in a series describing the results of a program attempting to fit models of the brightness distribution to radio sources observed at 365 MHz with the Bandwidth Synthesis Interferometer (BSI) operated by the University of Texas Radio Astronomy Observatory. Results for a further 2918 radio sources are given. An unresolved model and three symmetric extended models with angular sizes in the range 10--70 arcsec were attempted for each radio source. In addition, for 348 sources for which other observations of brightness distribution are published, the reference to the observations and a brief description are included

  4. Creating infinite contrast in fluorescence microscopy by using lanthanide centered emission

    DEFF Research Database (Denmark)

    R. Carro-Temboury, Miguel; Arppe, Riikka Matleena; Hempel, Casper

    2017-01-01

    The popularity of fluorescence microscopy arises from the inherent mode of action, where the fluorescence emission from probes is used to visualize selected features on a presumed dark background. However, the background is rarely truly dark, and image processing and analysis is needed to enhance...... the fluorescent signal that is ascribed to the selected feature. The image acquisition is facilitated by using considerable illumination, bright probes at a relatively high concentration in order to make the fluorescent signal significantly more intense than the background signal. Here, we present two methods......, while method II resolves the fluorescent signal by subtracting a background calculated via the gradient. Both methods improve signal-to-background ratio significantly and we suggest that spectral imaging of lanthanide-centered emission can be used as a tool to obtain absolute contrast in bioimaging....

  5. The Positively Charged Hyperbranched Polymers with Tunable Fluorescence and the Cell Imaging Application.

    Science.gov (United States)

    Ma, Hengchang; Qin, Yanfang; Yang, Zenming; Yang, Manyi; Ma, Yucheng; Yin, Pei; Yang, Yuan; Wang, Tao; Lei, Ziqiang; Yao, Xiaoqiang

    2018-04-25

    Fluorescence-tunable materials are becoming increasingly attractive for their potential application in optics, electronics, and biomedical technology. Herein, a multi-color molecular pixel system is realized using simple copolymerization method. Bleeding both of complementary colors from blue and yellow fluorescence segments, reproduced a serious multicolor fluorescence materials. Interestingly, the emission colors of the polymers can be fine-tuned in solid state, solution phase, and in hydrogel state. More importantly, the positive fluorescent polymers exhibited cell-membrane permeable ability, and were found to accumulate on the cell nucleus, exhibiting remarkable selectivity to give bright fluorescence. The DNA/RNA selectivity experiments in vitro and in vivo verified that [tris(4-(pyridin-4-yl)phenyl)amine]-[1,8-dibromooctane] (TPPA-DBO) has prominent selectivity to DNA over RNA inside cells.

  6. Fluorescence and Spectral Imaging

    Directory of Open Access Journals (Sweden)

    Ralph S. DaCosta

    2007-01-01

    Full Text Available Early identification of dysplasia remains a critical goal for diagnostic endoscopy since early discovery directly improves patient survival because it allows endoscopic or surgical intervention with disease localized without lymph node involvement. Clinical studies have successfully used tissue autofluorescence with conventional white light endoscopy and biopsy for detecting adenomatous colonic polyps, differentiating benign hyperplastic from adenomas with acceptable sensitivity and specificity. In Barrett's esophagus, the detection of dysplasia remains problematic because of background inflammation, whereas in the squamous esophagus, autofluorescence imaging appears to be more dependable. Point fluorescence spectroscopy, although playing a crucial role in the pioneering mechanistic development of fluorescence endoscopic imaging, does not seem to have a current function in endoscopy because of its nontargeted sampling and suboptimal sensitivity and specificity. Other point spectroscopic modalities, such as Raman spectroscopy and elastic light scattering, continue to be evaluated in clinical studies, but still suffer the significant disadvantages of being random and nonimaging. A recent addition to the fluorescence endoscopic imaging arsenal is the use of confocal fluorescence endomicroscopy, which provides real-time optical biopsy for the first time. To improve detection of dysplasia in the gastrointestinal tract, a new and exciting development has been the use of exogenous fluorescence contrast probes that specifically target a variety of disease-related cellular biomarkers using conventional fluorescent dyes and novel potent fluorescent nanocrystals (i.e., quantum dots. This is an area of great promise, but still in its infancy, and preclinical studies are currently under way.

  7. Mineralogical characterization and beneficiation study of kaolin from Equador (RN) and Junco do Serido (PB) to increase the brightness index

    International Nuclear Information System (INIS)

    Campos, V.M.J.S.; Bertolino, L.C.; Alves, O.C.

    2017-01-01

    Kaolin is a rock composed mainly of kaolinite. It is used in many industrial segments, such as paper and ceramics. However, for these uses it is necessary to submit the ore to appropriate beneficiation, which generally involves magnetic separation and chemical bleaching, aiming to remove iron oxide and hydroxide, raising brightness index. This work reports the mineralogical characterization and analysis of the beneficiation of three samples of kaolin, two from Equador (Rio Grande do Norte - RN) and the third from Junco do Serido (Paraiba - PB). The samples were submitted to granulometric classification in sieve of 44 μm, magnetic separation in a magnetic induction of 14000 gauss, and chemical bleaching with sodium dithionite during 240 min. The processes were divided into two beneficiation routes. X-ray diffraction, chemical analysis by X-ray fluorescence and scanning electron microscopy were used for mineralogical characterization of the ore and measurement of the brightness index, while electron paramagnetic resonance was applied to study the variation of iron oxides and hydroxides during beneficiation. The results indicate that just 30 min of chemical bleaching without magnetic separation was sufficient to increase the brightness index from 78.2 to 90.2% and from 91.3 to 95.7% in the two samples from Equador (RN) and from 86.9 to 90.4% in the sample from Junco do Serido (PB). The magnetic separation, although causing a small increase in the brightness index, was inefficient for removal of iron oxides and hydroxides. The results indicated no need for using magnetic separation in plants for beneficiation of kaolin from these two locations, providing the reduction of production costs allowing better exploitation of the ore. (author)

  8. High-performance hybrid white organic light-emitting devices without interlayer between fluorescent and phosphorescent emissive regions.

    Science.gov (United States)

    Sun, Ning; Wang, Qi; Zhao, Yongbiao; Chen, Yonghua; Yang, Dezhi; Zhao, Fangchao; Chen, Jiangshan; Ma, Dongge

    2014-03-12

    By using mixed hosts with bipolar transport properties for blue emissive layers, a novel phosphorescence/fluorescence hybrid white OLED without using an interlayer between the fluorescent and phosphorescent regions is demonstrated. The peak EQE of the device is 19.0% and remains as high as 17.0% at the practical brightness of 1000 cd m(-2) . © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Fluorescent discharge lamp

    Science.gov (United States)

    Mukai, E.; Otsuka, H.; Nomi, K.; Honmo, I.

    1982-01-01

    A rapidly illuminating fluorescent lamp 1,200 mm long and 32.5 mm in diameter with an interior conducting strip which is compatible with conventional fixtures and ballasts is described. The fluorescent lamp is composed of a linear glass tube, electrodes sealed at both ends, mercury and raregas sealed in the glass tube, a fluorescent substance clad on the inner walls of the glass tube, and a clad conducting strip extending the entire length of the glass tube in the axial direction on the inner surface of the tube.

  10. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M [Santa Fe, NM; Waldo, Geoffrey S [Santa Fe, NM; Kiss, Csaba [Los Alamos, NM

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  11. Bright infrared LEDs based on colloidal quantum-dots

    KAUST Repository

    Sun, Liangfeng; Choi, Joshua J.; Stachnik, David; Bartnik, Adam C.; Hyun, Byung-Ryool; Malliaras, George G.; Hanrath, Tobias; Wise, Frank W.

    2013-01-01

    Record-brightness infrared LEDs based on colloidal quantum-dots have been achieved through control of the spacing between adjacent quantum-dots. By tuning the size of quantum-dots, the emission wavelengths can be tuned between 900nm and 1650nm. © 2013 Materials Research Society.

  12. The star-bright hour : [luuletused] / Betti Alver

    Index Scriptorium Estoniae

    Alver, Betti, 1906-1989

    2006-01-01

    Sisu: The star-bright hour ; Not a dream ; The Piper ; Corals in an ancent river. Luuletused pärinevad kogumikust "Tuulelaeval valgusest on aerud = Windship with Oars of Light. (Tallinn : Huma, 2001). Orig.: Tähetund ; Mitte viirastus, meelepett ; Vilepuhuja ; Korallid Emajões

  13. Optical variability of the medium-bright quasar sample

    International Nuclear Information System (INIS)

    Huang, K.; Mitchell, K.J.; Usher, P.D.

    1990-01-01

    A variability study of the 32-member Medium-Bright Quasar Sample is reported. It is found that the star US 1953 has undergone a noticeable variation in the course of 26 hr. Apparent variations in the extragalactic object US 3498 may be illusory, owing to its partially resolved appearance. No other evidence for variability was detected. 34 refs

  14. A surface brightness analysis of eight RR Lyrae stars

    International Nuclear Information System (INIS)

    Hawley, S.L.; Barnes, T.G. III; Moffett, T.J.

    1987-01-01

    The authors have used a surface brightness, (V-R) relation to analyze new contemporaneous photometry and radial velocity data for 6 RR-ab type stars and to re-analyze previously published data for RR Lyrae and X Arietis. Systematic effects were found in the surface brightness at phases near minimum radius. Excluding these phases, they determine the slope of the surface brightness relation and the mean radius for each star. They also find a zero point which includes both a distance term and the zero point of the surface brightness relation. The sample includes stars with Preston's metallicity indicator ΔS = 0 to 9, with periods ranging from 0.397 days to 0.651 days. Their results indicate a log(R/R solar ) vs. log P relation in the sense that stars with longer periods have larger radii, in agreement with theoretical predictions. Their radii are consistent with bolometric magnitudes in the range 0.2 - 0.8 magnitude but accurate magnitudes must await a reliable T e - color calibration

  15. Response of noctilucent cloud brightness to daily solar variations

    Science.gov (United States)

    Dalin, P.; Pertsev, N.; Perminov, V.; Dubietis, A.; Zadorozhny, A.; Zalcik, M.; McEachran, I.; McEwan, T.; Černis, K.; Grønne, J.; Taustrup, T.; Hansen, O.; Andersen, H.; Melnikov, D.; Manevich, A.; Romejko, V.; Lifatova, D.

    2018-04-01

    For the first time, long-term data sets of ground-based observations of noctilucent clouds (NLC) around the globe have been analyzed in order to investigate a response of NLC to solar UV irradiance variability on a day-to-day scale. NLC brightness has been considered versus variations of solar Lyman-alpha flux. We have found that day-to-day solar variability, whose effect is generally masked in the natural NLC variability, has a statistically significant effect when considering large statistics for more than ten years. Average increase in day-to-day solar Lyman-α flux results in average decrease in day-to-day NLC brightness that can be explained by robust physical mechanisms taking place in the summer mesosphere. Average time lags between variations of Lyman-α flux and NLC brightness are short (0-3 days), suggesting a dominant role of direct solar heating and of the dynamical mechanism compared to photodissociation of water vapor by solar Lyman-α flux. All found regularities are consistent between various ground-based NLC data sets collected at different locations around the globe and for various time intervals. Signatures of a 27-day periodicity seem to be present in the NLC brightness for individual summertime intervals; however, this oscillation cannot be unambiguously retrieved due to inevitable periods of tropospheric cloudiness.

  16. Matter-wave bright solitons in effective bichromatic lattice potentials

    Indian Academy of Sciences (India)

    Matter-wave bright solitons in bichromatic lattice potentials are considered and their dynamics for different lattice environments are studied. Bichromatic potentials are created from superpositions of (i) two linear optical lattices and (ii) a linear and a nonlinear optical lattice. Effective potentials are found for the solitons in both ...

  17. Matter wave interference pattern in the collision of bright solitons

    International Nuclear Information System (INIS)

    Kumar, V. Ramesh; Radha, R.; Panigrahi, Prasanta K.

    2009-01-01

    We investigate the dynamics of Bose-Einstein condensates in a quasi one-dimensional regime in a time-dependent trap and show analytically that it is possible to observe matter wave interference patterns in the intra-trap collision of two bright solitons by selectively tuning the trap frequency and scattering length.

  18. The morphology and surface brightness of extragalactic jets

    International Nuclear Information System (INIS)

    Bicknell, G.V.

    1983-01-01

    The problems associated with laminar flow models are reviewed, and an analogy between laboratory jets and astrophysical jets is given. The relationship between surface brightness and the jet full width half maximum is not in general as predicted by simple magnetohydrodynamic models. An alternative turbulent model is presented

  19. Minimum-phase distribution of cosmic source brightness

    International Nuclear Information System (INIS)

    Gal'chenko, A.A.; Malov, I.F.; Mogil'nitskaya, L.F.; Frolov, V.A.

    1984-01-01

    Minimum-phase distributions of brightness (profiles) for cosmic radio sources 3C 144 (the wave lambda=21 cm), 3C 338 (lambda=3.5 m), and 3C 353 (labda=31.3 cm and 3.5 m) are obtained. A real possibility for the profile recovery from module fragments of its Fourier-image is shown

  20. The "Brightness Rules" Alternative Conception for Light Bulb Circuits

    Science.gov (United States)

    Bryan, Joel A.; Stuessy, Carol

    2006-01-01

    An alternative conception for the observed differences in light bulb brightness was revealed during an unguided inquiry investigation in which prospective elementary teachers placed identical bulbs in series, parallel, and combination direct current circuits. Classroom observations, document analyses, and video and audio transcriptions led to the…

  1. The star-bright hour : [poems] / Betti Alver

    Index Scriptorium Estoniae

    Alver, Betti, 1906-1989

    2003-01-01

    Autori lühitutvustus lk. 231. Sisu: The star-bright hour ; The debt ; Not a dream ; Fog-bound ; Corals in an Ancient river ; Frou-frou 1-3. Orig.: Tähetund ; Vilepuhuja ; Võlg ; "Mitte viirastus, meelepett..." ; Udus ; Korallid Emajões ; Froufrou 1-3

  2. High-brightness fiber-coupled pump laser development

    Science.gov (United States)

    Price, Kirk; Karlsen, Scott; Leisher, Paul; Martinsen, Robert

    2010-02-01

    We report on the continued development of high brightness laser diode modules at nLIGHT Photonics. These modules, based on nLIGHT's PearlTM product platform, demonstrate excellence in output power, brightness, wavelength stabilization, and long wavelength performance. This system, based on 14 single emitters, is designed to couple diode laser light into a 105 μm fiber at an excitation NA of under 0.14. We demonstrate over 100W of optical power at 9xx nm with a diode brightness exceeding 20 MW/cm2-str with an operating efficiency of approximately 50%. Additional results show over 70W of optical coupled at 8xx nm. Record brilliance at wavelengths 14xx nm and longer will also be demonstrated, with over 15 W of optical power with a beam quality of 7.5 mm-mrad. These results of high brightness, high efficiency, and wavelength stabilization demonstrate the pump technology required for next generation solid state and fiber lasers.

  3. Near-infrared photometry of bright elliptical galaxies

    NARCIS (Netherlands)

    Peletier, R. F.; Valentijn, E. A.; Jameson, R. F.

    High-quality visual-infrared color profiles have been determined for elliptical galaxies for the first time. Surface photometry in J and K is presented for 12 bright elliptical galaxies, and the results have been combined with CCD data in visual passbands. It is shown that the galaxies become bluer

  4. 150 southern compact and bright-nucleus galaxies

    International Nuclear Information System (INIS)

    Fairall, A.P.

    1977-01-01

    Galaxies having regions of exceptionally high surface brightness have been selected from the ESO Quick Blue Survey and investigated by 'grating photography' -direct photography plus low-dispersion slitless spectroscopy. Two new Seyfert galaxies and a peculiar multiple system have been discovered. Differences in red continua are also noted. (author)

  5. Bright to dim oscillatory response of the Neurospora circadian oscillator.

    Science.gov (United States)

    Gooch, Van D; Johnson, Alicia E; Larrondo, Luis F; Loros, Jennifer J; Dunlap, Jay C

    2014-02-01

    The fungus Neurospora crassa constitutes an important model system extensively used in chronobiology. Several studies have addressed how environmental cues, such as light, can reset or synchronize a circadian system. By means of an optimized firefly luciferase reporter gene and a controllable lighting system, we show that Neurospora can display molecular circadian rhythms in dim light when cultures receive bright light prior to entering dim light conditions. We refer to this behavior as the "bright to dim oscillatory response" (BDOR). The bright light treatment can be applied up to 76 h prior to dim exposure, and it can be as short as 15 min in duration. We have characterized this response in respect to the duration of the light pulse, the time of the light pulse before dim, the intensity of dim light, and the oscillation dynamics in dim light. Although the molecular mechanism that drives the BDOR remains obscure, these findings suggest that a long-term memory of bright light exists as part of the circadian molecular components. It is important to consider the ecological significance of such dim light responses in respect to how organisms naturally maintain their timing mechanism in moonlight.

  6. Progress in extremely high brightness LED-based light sources

    Science.gov (United States)

    Hoelen, Christoph; Antonis, Piet; de Boer, Dick; Koole, Rolf; Kadijk, Simon; Li, Yun; Vanbroekhoven, Vincent; Van De Voorde, Patrick

    2017-09-01

    Although the maximum brightness of LEDs has been increasing continuously during the past decade, their luminance is still far from what is required for multiple applications that still rely on the high brightness of discharge lamps. In particular for high brightness applications with limited étendue, e.g. front projection, only very modest luminance values in the beam can be achieved with LEDs compared to systems based on discharge lamps or lasers. With dedicated architectures, phosphor-converted green LEDs for projection may achieve luminance values up to 200-300 Mnit. In this paper we report on the progress made in the development of light engines based on an elongated luminescent concentrator pumped by blue LEDs. This concept has recently been introduced to the market as ColorSpark High Lumen Density LED technology. These sources outperform the maximum brightness of LEDs by multiple factors. In LED front projection, green LEDs are the main limiting factor. With our green modules, we now have achieved peak luminance values of 2 Gnit, enabling LED-based projection systems with over 4000 ANSI lm. Extension of this concept to yellow and red light sources is presented. The light source efficiency has been increased considerably, reaching 45-60 lm/W for green under practical application conditions. The module architecture, beam shaping, and performance characteristics are reviewed, as well as system aspects. The performance increase, spectral range extensions, beam-shaping flexibility, and cost reductions realized with the new module architecture enable a breakthrough in LED-based projection systems and in a wide variety of other high brightness applications.

  7. SKYMONITOR: A Global Network for Sky Brightness Measurements

    Science.gov (United States)

    Davis, Donald R.; Mckenna, D.; Pulvermacher, R.; Everett, M.

    2010-01-01

    We are implementing a global network to measure sky brightness at dark-sky critical sites with the goal of creating a multi-decade database. The heart of this project is the Night Sky Brightness Monitor (NSBM), an autonomous 2 channel photometer which measures night sky brightness in the visual wavelengths (Mckenna et al, AAS 2009). Sky brightness is measured every minute at two elevation angles typically zenith and 20 degrees to monitor brightness and transparency. The NSBM consists of two parts, a remote unit and a base station with an internet connection. Currently these devices use 2.4 Ghz transceivers with a range of 100 meters. The remote unit is battery powered with daytime recharging using a solar panel. Data received by the base unit is transmitted via email protocol to IDA offices in Tucson where it will be collected, archived and made available to the user community via a web interface. Two other versions of the NSBM are under development: one for radio sensitive areas using an optical fiber link and the second that reads data directly to a laptop for sites without internet access. NSBM units are currently undergoing field testing at two observatories. With support from the National Science Foundation, we will construct and install a total of 10 units at astronomical observatories. With additional funding, we will locate additional units at other sites such as National Parks, dark-sky preserves and other sites where dark sky preservation is crucial. We will present the current comparison with the National Park Service sky monitoring camera. We anticipate that the SKYMONITOR network will be functioning by the end of 2010.

  8. Diode lasers optimized in brightness for fiber laser pumping

    Science.gov (United States)

    Kelemen, M.; Gilly, J.; Friedmann, P.; Hilzensauer, S.; Ogrodowski, L.; Kissel, H.; Biesenbach, J.

    2018-02-01

    In diode laser applications for fiber laser pumping and fiber-coupled direct diode laser systems high brightness becomes essential in the last years. Fiber coupled modules benefit from continuous improvements of high-power diode lasers on chip level regarding output power, efficiency and beam characteristics resulting in record highbrightness values and increased pump power. To gain high brightness not only output power must be increased, but also near field widths and far field angles have to be below a certain value for higher power levels because brightness is proportional to output power divided by beam quality. While fast axis far fields typically show a current independent behaviour, for broadarea lasers far-fields in the slow axis suffer from a strong current and temperature dependence, limiting the brightness and therefore their use in fibre coupled modules. These limitations can be overcome by carefully optimizing chip temperature, thermal lensing and lateral mode structure by epitaxial and lateral resonator designs and processing. We present our latest results for InGaAs/AlGaAs broad-area single emitters with resonator lengths of 4mm emitting at 976nm and illustrate the improvements in beam quality over the last years. By optimizing the diode laser design a record value of the brightness for broad-area lasers with 4mm resonator length of 126 MW/cm2sr has been demonstrated with a maximum wall-plug efficiency of more than 70%. From these design also pump modules based on 9 mini-bars consisting of 5 emitters each have been realized with 360W pump power.

  9. Reviews in fluorescence 2007

    CERN Document Server

    Lakowicz, Joseph R; Geddes, Chris D

    2009-01-01

    This fourth volume in the Springer series summarizes the year's progress in fluorescence, with authoritative analytical reviews specialized enough for professional researchers, yet also appealing to a wider audience of scientists in related fields.

  10. Introduction to fluorescence

    CERN Document Server

    Jameson, David M

    2014-01-01

    "An essential contribution to educating scientists in the principles of fluorescence. It will also be an important addition to the libraries of practitioners applying the principles of molecular fluorescence."-Ken Jacobson, Kenan Distinguished Professor of Cell Biology and Physiology, University of North Carolina at Chapel Hill"An exquisite compendium of fluorescence and its applications in biochemistry enriched by a very exciting historical perspective. This book will become a standard text for graduate students and other scientists."-Drs. Zygmunt (Karol) Gryczynski and Ignacy Gryczynski, University of North Texas Health Science Center"… truly a masterwork, combining clarity, precision, and good humor. The reader, novice or expert, will be pleased with the text and will not stop reading. It is a formidable account of the fluorescence field, which has impacted the life sciences so considerably in the last 60 years."-Jerson L. Silva, M.D., Ph.D., Professor and Director, National Institute of Science and Tech...

  11. Fluorescence (Multiwave) Confocal Microscopy.

    Science.gov (United States)

    Welzel, J; Kästle, Raphaela; Sattler, Elke C

    2016-10-01

    In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the depiction of certain structures, like epithelial tumors, nerves, and glands. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. High-Brightness Blue Light-Emitting Diodes Enabled by a Directly Grown Graphene Buffer Layer.

    Science.gov (United States)

    Chen, Zhaolong; Zhang, Xiang; Dou, Zhipeng; Wei, Tongbo; Liu, Zhiqiang; Qi, Yue; Ci, Haina; Wang, Yunyu; Li, Yang; Chang, Hongliang; Yan, Jianchang; Yang, Shenyuan; Zhang, Yanfeng; Wang, Junxi; Gao, Peng; Li, Jinmin; Liu, Zhongfan

    2018-06-08

    Single-crystalline GaN-based light-emitting diodes (LEDs) with high efficiency and long lifetime are the most promising solid-state lighting source compared with conventional incandescent and fluorescent lamps. However, the lattice and thermal mismatch between GaN and sapphire substrate always induces high stress and high density of dislocations and thus degrades the performance of LEDs. Here, the growth of high-quality GaN with low stress and a low density of dislocations on graphene (Gr) buffered sapphire substrate is reported for high-brightness blue LEDs. Gr films are directly grown on sapphire substrate to avoid the tedious transfer process and GaN is grown by metal-organic chemical vapor deposition (MOCVD). The introduced Gr buffer layer greatly releases biaxial stress and reduces the density of dislocations in GaN film and In x Ga 1- x N/GaN multiple quantum well structures. The as-fabricated LED devices therefore deliver much higher light output power compared to that on a bare sapphire substrate, which even outperforms the mature process derived counterpart. The GaN growth on Gr buffered sapphire only requires one-step growth, which largely shortens the MOCVD growth time. This facile strategy may pave a new way for applications of Gr films and bring several disruptive technologies for epitaxial growth of GaN film and its applications in high-brightness LEDs. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Testing the utility of fluorescent proteins in Mimulus lewisii by an Agrobacterium-mediated transient assay.

    Science.gov (United States)

    Ding, Baoqing; Yuan, Yao-Wu

    2016-04-01

    The Agrobacterium -mediated transient expression assay by leaf infiltration in Mimulus lewisii is robust. Fluorescent proteins EGFP, EYFP and DsRed give bright fluorescence signals in the infiltrated tissue. Mimulus lewisii is an emerging developmental genetic model system. Recently developed genomic and genetic resources and a stable transformation protocol have greatly facilitated the identification and functional characterization of genes controlling the development of ecologically important floral traits using this species. To further expedite gene and protein function analyses in M. lewisii, we adopted and simplified the Agrobacterium-mediated transient gene expression method routinely used in tobacco plants. With the validated transient assay, we examined the performance of fluorescent proteins EGFP, EYFP and DsRed in M. lewisii. All three proteins gave bright fluorescence signals when transiently expressed in agroinfiltrated leaves. Furthermore, we demonstrated the utility of fluorescent proteins in M. lewisii by showing the nuclear localization of Reduced Carotenoid Pigmentation 1 (RCP1), a recently discovered R2R3-MYB transcription factor that regulates carotenoid pigmentation during flower development. Both the transient assay and the fluorescent proteins are valuable additions to the M. lewisii toolbox, making this emerging genetic and developmental model system even more powerful.

  14. Fluorescent Staining of Tea Pathogenic Fungi in Tea Leaves Using Fluorescein-labeled Lectin

    Science.gov (United States)

    Yamada, Kengo; Yoshida, Katsuyuki; Sonoda, Ryoichi

    Fluorochrome-labeled lectin, fluorescein conjugated wheat germ agglutinin (F-WGA) was applied to stain tea pathogenic fungi in tea leaf tissue. Infected leaves were fixed and decolorized with a mixture of ethanol and acetic acid, and cleared with 10% KOH for whole mount before staining with F-WGA. Hyphae of Pestalotiopsis longiseta, Pseudocercospora ocellata, Botrytis cinerea and Colletotrichum theae-sinensis fluoresced brightly in whole mount and sectioned samples of infected leaf tissue. In browned tissue, hyphae did not fluoresce frequently in whole mount sample. Autofluorescence of leaf tissue was strong in browned tissue of sections, it was removed by 10% KOH treatment before staining. Penetration hyphae of C. theae-sinensis in cell wall of trichome and hyphae in basal part of trichome did not fluoresced frequently. In whole mount samples of tea leaf infected with Exobasidium vexans and E. reticulatum, hymenia appeared on leaf surface fluoresced, but hyphae in leaf tissue did not fluoresce. In sectioned samples, hyphae fluoresced brightly when sections were treated with 10% KOH before staining.

  15. LARGER PLANET RADII INFERRED FROM STELLAR ''FLICKER'' BRIGHTNESS VARIATIONS OF BRIGHT PLANET-HOST STARS

    International Nuclear Information System (INIS)

    Bastien, Fabienne A.; Stassun, Keivan G.; Pepper, Joshua

    2014-01-01

    Most extrasolar planets have been detected by their influence on their parent star, typically either gravitationally (the Doppler method) or by the small dip in brightness as the planet blocks a portion of the star (the transit method). Therefore, the accuracy with which we know the masses and radii of extrasolar planets depends directly on how well we know those of the stars, the latter usually determined from the measured stellar surface gravity, log g. Recent work has demonstrated that the short-timescale brightness variations ( f licker ) of stars can be used to measure log g to a high accuracy of ∼0.1-0.2 dex. Here, we use flicker measurements of 289 bright (Kepmag < 13) candidate planet-hosting stars with T eff = 4500-6650 K to re-assess the stellar parameters and determine the resulting impact on derived planet properties. This re-assessment reveals that for the brightest planet-host stars, Malmquist bias contaminates the stellar sample with evolved stars: nearly 50% of the bright planet-host stars are subgiants. As a result, the stellar radii, and hence the radii of the planets orbiting these stars, are on average 20%-30% larger than previous measurements had suggested

  16. Fluorescence Image Segmentation by using Digitally Reconstructed Fluorescence Images

    OpenAIRE

    Blumer, Clemens; Vivien, Cyprien; Oertner, Thomas G; Vetter, Thomas

    2011-01-01

    In biological experiments fluorescence imaging is used to image living and stimulated neurons. But the analysis of fluorescence images is a difficult task. It is not possible to conclude the shape of an object from fluorescence images alone. Therefore, it is not feasible to get good manual segmented nor ground truth data from fluorescence images. Supervised learning approaches are not possible without training data. To overcome this issues we propose to synthesize fluorescence images and call...

  17. Laser fluorescence bronchoscope for localization of occult lung tumors

    International Nuclear Information System (INIS)

    Profio, A.E.; Doiron, D.R.; King, E.G.

    1979-01-01

    A system for imaging occult bronchogenic carcinoma by the fluorescence of previously-injected, tumor-specific compound hematoporphyrin-derivative has been assembled and successfully used to locate a tumor l mm thick. The violet excitation source is a krypton ion laser coupled to fused quartz fiber light conductor. An electrostatic image intensifier attached to a standard flexible fiberoptic bronchoscope provides a bright image even at relatively low irradiance. A red secondary filter rejects most reflected background and autofluorescence. Sensitivity and contrast capability of the system should permit detection of a tumor less than 0.1 mm thick

  18. The relationship between brightness temperature and soil moisture. Selection of frequency range for microwave remote sensing

    International Nuclear Information System (INIS)

    Rao, K.S.; Chandra, G.; Rao, P.V.N.

    1987-01-01

    The analysis of brightness temperature data acquired from field and aircraft experiments demonstrates a linear relationship between soil moisture and brightness temperature. However, the analysis of brightness temperature data acquired by the Skylab radiometer demonstrates a non-linear relationship between soil moisture and brightness temperature. In view of the above and also because of recent theoretical developments for the calculation of the dielectric constant and brightness temperature under varying soil moisture profile conditions, an attempt is made to study the theoretical relationship between brightness temperature and soil moisture as a function of frequency. Through the above analysis, the appropriate microwave frequency range for soil moisture studies is recommended

  19. Red fluorescence increases with depth in reef fishes, supporting a visual function, not UV protection

    Science.gov (United States)

    Meadows, Melissa G.; Anthes, Nils; Dangelmayer, Sandra; Alwany, Magdy A.; Gerlach, Tobias; Schulte, Gregor; Sprenger, Dennis; Theobald, Jennifer; Michiels, Nico K.

    2014-01-01

    Why do some marine fishes exhibit striking patterns of natural red fluorescence? In this study, we contrast two non-exclusive hypotheses: (i) that UV absorption by fluorescent pigments offers significant photoprotection in shallow water, where UV irradiance is strongest; and (ii) that red fluorescence enhances visual contrast at depths below −10 m, where most light in the ‘red’ 600–700 nm range has been absorbed. Whereas the photoprotection hypothesis predicts fluorescence to be stronger near the surface and weaker in deeper water, the visual contrast hypothesis predicts the opposite. We used fluorometry to measure red fluorescence brightness in vivo in individuals belonging to eight common small reef fish species with conspicuously red fluorescent eyes. Fluorescence was significantly brighter in specimens from the −20 m sites than in those from −5 m sites in six out of eight species. No difference was found in the remaining two. Our results support the visual contrast hypothesis. We discuss the possible roles fluorescence may play in fish visual ecology and highlight the possibility that fluorescent light emission from the eyes in particular may be used to detect cryptic prey. PMID:25030989

  20. Nine New Fluorescent Probes

    Science.gov (United States)

    Lin, Tsung-I.; Jovanovic, Misa V.; Dowben, Robert M.

    1989-06-01

    Absorption and fluorescence spectroscopic studies are reported here for nine new fluorescent probes recently synthesized in our laboratories: four pyrene derivatives with substituents of (i) 1,3-diacetoxy-6,8-dichlorosulfonyl, (ii) 1,3-dihydroxy-6,8-disodiumsulfonate, (iii) 1,3-disodiumsulfonate, and (iv) l-ethoxy-3,6,8-trisodiumsulfonate groups, and five [7-julolidino] coumarin derivatives with substituents of (v) 3-carboxylate-4-methyl, (vi) 3- methylcarboxylate, (vii) 3-acetate-4-methyl, (viii) 3-propionate-4-methyl, and (ix) 3-sulfonate-4-methyl groups. Pyrene compounds i and ii and coumarin compounds v and vi exhibit interesting absorbance and fluorescence properties: their absorption maxima are red shifted compared to the parent compound to the blue-green region, and the band width broadens considerably. All four blue-absorbing dyes fluoresce intensely in the green region, and the two pyrene compounds emit at such long wavelengths without formation of excimers. The fluorescence properties of these compounds are quite environment-sensitive: considerable spectral shifts and fluorescence intensity changes have been observed in the pH range from 3 to 10 and in a wide variety of polar and hydrophobic solvents with vastly different dielectric constants. The high extinction and fluorescence quantum yield of these probes make them ideal fluorescent labeling reagents for proteins, antibodies, nucleic acids, and cellular organelles. The pH and hydrophobicity-dependent fluorescence changes can be utilized as optical pH and/or hydrophobicity indicators for mapping environmental difference in various cellular components in a single cell. Since all nine probes absorb in the UV, but emit at different wavelengths in the visible, these two groups of compounds offer an advantage of utilizing a single monochromatic light source (e.g., a nitrogen laser) to achieve multi-wavelength detection for flow cytometry application. As a first step to explore potential application in

  1. Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.

    Science.gov (United States)

    Niko, Yosuke; Didier, Pascal; Mely, Yves; Konishi, Gen-ichi; Klymchenko, Andrey S

    2016-01-11

    Imaging lipid organization in cell membranes requires advanced fluorescent probes. Here, we show that a recently synthesized push-pull pyrene (PA), similarly to popular probe Laurdan, changes the emission maximum as a function of lipid order, but outperforms it by spectroscopic properties. In addition to red-shifted absorption compatible with common 405 nm diode laser, PA shows higher brightness and much higher photostability than Laurdan in apolar membrane environments. Moreover, PA is compatible with two-photon excitation at wavelengths >800 nm, which was successfully used for ratiometric imaging of coexisting liquid ordered and disordered phases in giant unilamellar vesicles. Fluorescence confocal microscopy in Hela cells revealed that PA efficiently stains the plasma membrane and the intracellular membranes at >20-fold lower concentrations, as compared to Laurdan. Finally, ratiometric imaging using PA reveals variation of lipid order within different cellular compartments: plasma membranes are close to liquid ordered phase of model membranes composed of sphingomyelin and cholesterol, while intracellular membranes are much less ordered, matching well membranes composed of unsaturated phospholipids without cholesterol. These differences in the lipid order were confirmed by fluorescence lifetime imaging (FLIM) at the blue edge of PA emission band. PA probe constitutes thus a new powerful tool for biomembrane research.

  2. Improving the laser brightness of a commercial laser system

    Science.gov (United States)

    Naidoo, Darryl; Litvin, Igor; Forbes, Andrew

    2016-02-01

    We investigate the selection of a flat-top beam and a Gaussian beam inside a laser cavity on opposing mirrors. The concept is tested external to the laser cavity in a single pass and double pass regime where the latter mimics a single round trip in the laser. We implement this intra-cavity selection through the use of two 16 level diffractive optical elements. We consider a solid-state diode side-pumped laser resonator in a typical commercial laser configuration that consists of two planar mirrors where the DOEs are positioned at the mirrors. We out couple the Gaussian and flat-top distributions and we show that we improve the brightness of the laser with active mode control. We also demonstrate that the quality of the beam transformations determine the brightness improvement.

  3. Modeling laser brightness from cross Porro prism resonators

    Science.gov (United States)

    Forbes, Andrew; Burger, Liesl; Litvin, Igor Anatolievich

    2006-08-01

    Laser brightness is a parameter often used to compare high power laser beam delivery from various sources, and incorporates both the power contained in the particular mode, as well as the propagation of that mode through the beam quality factor, M2. In this study a cross Porro prism resonator is considered; crossed Porro prism resonators have been known for some time, but until recently have not been modeled as a complete physical optics system that allows the modal output to be determined as a function of the rotation angle of the prisms. In this paper we consider the diffraction losses as a function of the prism rotation angle relative to one another, and combine this with the propagation of the specific modes to determine the laser output brightness as a function of the prism orientation.

  4. Optical Observations of X-ray Bright, Optically Normal Galaxies

    Science.gov (United States)

    Sadun, Alberto C.; Aryan, N. S.; Ghosh, K. K.

    2007-05-01

    X-ray bright, optically normal galaxies (XBONGs) are galaxies that seem to have normal spectra and morphology, but are relatively bright x-ray sources. The large ratio of the x-ray to optical emission suggests that some activity, similar to that of active galactic nuclei (AGN), is occurring. Since the galaxies do not show any obvious sign of nuclear activity in their optical spectra, one possible explanation is that these galaxies do not have an optically thick accretion disk at small radii, as previously assumed. Previous data for NGC 7626 classifies it as an XBONG, and so we are studying optical features of this galaxy in order to determine better its features. After confirming an x-ray jet, we are now comparing this to optical features that we have found, including warped dust lanes and a possible optical jet.

  5. Large-scale brightness inhomogeneities in the solar atmosphere

    International Nuclear Information System (INIS)

    Mitchell, W.E. Jr.

    1982-01-01

    The intensity residuals are analyzed from a series of solar limb-darkening measurements in the wavelength range 5656 to 2997 A. The lengths of residual strings of the same sign exceed expectation by several orders of magnitude. The power spectrum fo the residuals shows a weak excess around 6000 km. For further study the 34000 limb-darkening residuals are subdivided into 5100 bright and faint cells. The frequency distribution of cell sizes peaks around 4500 km and increases from center to limb, the faint cells showing the greater center-limb effect. The cells are also studied as to contrast. A synoptic view indicates that only 12% of the cells are identifiable after a half hour. Phenomena that may combine to produce the observed wide spectrum of brightness inhomogeneities are briefly discussed. (orig.)

  6. The ASAS-SN bright supernova catalogue - III. 2016

    Science.gov (United States)

    Holoien, T. W.-S.; Brown, J. S.; Stanek, K. Z.; Kochanek, C. S.; Shappee, B. J.; Prieto, J. L.; Dong, Subo; Brimacombe, J.; Bishop, D. W.; Bose, S.; Beacom, J. F.; Bersier, D.; Chen, Ping; Chomiuk, L.; Falco, E.; Godoy-Rivera, D.; Morrell, N.; Pojmanski, G.; Shields, J. V.; Strader, J.; Stritzinger, M. D.; Thompson, Todd A.; Woźniak, P. R.; Bock, G.; Cacella, P.; Conseil, E.; Cruz, I.; Fernandez, J. M.; Kiyota, S.; Koff, R. A.; Krannich, G.; Marples, P.; Masi, G.; Monard, L. A. G.; Nicholls, B.; Nicolas, J.; Post, R. S.; Stone, G.; Wiethoff, W. S.

    2017-11-01

    This catalogue summarizes information for all supernovae discovered by the All-Sky Automated Survey for SuperNovae (ASAS-SN) and all other bright (mpeak ≤ 17), spectroscopically confirmed supernovae discovered in 2016. We then gather the near-infrared through ultraviolet magnitudes of all host galaxies and the offsets of the supernovae from the centres of their hosts from public data bases. We illustrate the results using a sample that now totals 668 supernovae discovered since 2014 May 1, including the supernovae from our previous catalogues, with type distributions closely matching those of the ideal magnitude limited sample from Li et al. This is the third of a series of yearly papers on bright supernovae and their hosts from the ASAS-SN team.

  7. Recent advances in high-brightness electron guns at AES

    International Nuclear Information System (INIS)

    Bluem, H.; Todd, A.M.M.; Cole, M.D.; Rathke, J.; Schultheiss, T.

    2003-01-01

    We describe a number of active Advanced Energy Systems projects pertaining to the development of advanced, high-brightness electron guns for various applications. These projects include a fully superconducting, CW RF gun, nearing test, that utilizes the niobium surface as the photocathode material. An integrated 100 mA, low emittance DC/SRF gun, ideal as an injector for ERL-type light sources and intended as the injector for a 100 kW FEL, is in late design stage. A parallel high-power, CW, normal-conducting L-band RF gun project has just begun. The early performance analysis for this gun also shows good promise as an injector for ERL-type light sources. Lastly, a fully axisymmetric RF gun, operating in X-band, is being studied as a source of extremely bright electron bunches

  8. Bright breathers in nonlinear left-handed metamaterial lattices

    Science.gov (United States)

    Koukouloyannis, V.; Kevrekidis, P. G.; Veldes, G. P.; Frantzeskakis, D. J.; DiMarzio, D.; Lan, X.; Radisic, V.

    2018-02-01

    In the present work, we examine a prototypical model for the formation of bright breathers in nonlinear left-handed metamaterial lattices. Utilizing the paradigm of nonlinear transmission lines, we build a relevant lattice and develop a quasi-continuum multiscale approximation that enables us to appreciate both the underlying linear dispersion relation and the potential for bifurcation of nonlinear states. We focus here, more specifically, on bright discrete breathers which bifurcate from the lower edge of the linear dispersion relation at wavenumber k=π . Guided by the multiscale analysis, we calculate numerically both the stable inter-site centered and the unstable site-centered members of the relevant family. We quantify the associated stability via Floquet analysis and the Peierls-Nabarro barrier of the energy difference between these branches. Finally, we explore the dynamical implications of these findings towards the potential mobility or lack thereof (pinning) of such breather solutions.

  9. Very bright, near-infrared single photon emitters in diamond

    Directory of Open Access Journals (Sweden)

    D. W. M. Lau

    2013-09-01

    Full Text Available We demonstrate activation of bright diamond single photon emitters in the near infrared range by thermal annealing alone, i.e., without ion implantation. The activation is crucially dependent on the annealing ambient. The activation of the single photon emitters is only observed when the sample is annealed in forming gas (4% H2 in Ar above temperatures of 1000 °C. By contrast, no emitters are activated by annealing in vacuum, oxygen, argon or deuterium. The emitters activated by annealing in forming gas exhibit very bright emission in the 730-760 nm wavelength range and have linewidths of ∼1.5-2.5 nm at room temperature.

  10. High-brightness tapered laser diodes with photonic crystal structures

    Science.gov (United States)

    Li, Yi; Du, Weichuan; Kun, Zhou; Gao, Songxin; Ma, Yi; Tang, Chun

    2018-02-01

    Beam quality of tapered laser diodes is limited by higher order lateral mode. On purpose of optimizing the brightness of tapered laser diodes, we developed a novel design of tapered diodes. This devices based on InGaAs/AlGaAs asymmetry epitaxial structure, containing higher order lateral mode filtering schemes especially photonic crystal structures, which fabricated cost effectively by using standard photolithography and dry etch processes. Meanwhile, the effects of photonic crystal structures on mode control are also investigated theoretically by FDBPM (Finite-Difference Beam Propagation Method) calculation. We achieved a CW optical output power of 6.9W at 940nm for a single emitter with 4 mm cavity length. A nearly diffraction limited beam of M2 ≍1.9 @ 0.5W has been demonstrated, and a highest brightness of β =75MW/(cm2 ·sr) was reached.

  11. Novel fluorescent carbonic nanomaterials for sensing and imaging

    International Nuclear Information System (INIS)

    Demchenko, Alexander P; Dekaliuk, Mariia O

    2013-01-01

    Small brightly fluorescent carbon nanoparticles have emerged as a new class of materials important for sensing and imaging applications. We analyze comparatively the properties of nanodiamonds, graphene and graphene oxide ‘dots’, of modified carbon nanotubes and of diverse carbon nanoparticles known as ‘C-dots’ obtained by different methods. The mechanisms of their light absorption and luminescence emission are still unresolved and the arguments are presented for their common origin. Regarding present and potential applications, we provide critical comparison with the other types of fluorescence reporters, such as organic dyes and semiconductor quantum dots. Their most prospective applications in sensing (based on the changes of intensity, FRET and lifetime) and in imaging technologies on the level of living cells and whole bodies are overviewed. The possibilities for design on their basis of multifunctional nanocomposites on a broader scale of theranostics are outlined. (topical review)

  12. Using RSpec in an introductory bright star spectroscopy lab activity

    Science.gov (United States)

    Howe, James; Sitar, David J.

    2018-01-01

    After presenting at the North Carolina Section of the American Association of Physics Teachers during the fall 2016 meeting, we were encouraged to turn our poster into a paper. This article describes the strengthening of a bright star spectroscopy lab activity for introductory astronomy lab students (AST1002) at Appalachian State University. Explanations of the tools and methods used in the activity are included, particularly the preparation of additional materials using RSpec and calibrated instrument response curves.

  13. Manakins can produce iridescent and bright feather colours without melanosomes.

    Science.gov (United States)

    Igic, Branislav; D'Alba, Liliana; Shawkey, Matthew D

    2016-06-15

    Males of many species often use colourful and conspicuous ornaments to attract females. Among these, male manakins (family: Pipridae) provide classic examples of sexual selection favouring the evolution of bright and colourful plumage coloration. The highly iridescent feather colours of birds are most commonly produced by the periodic arrangement of melanin-containing organelles (melanosomes) within barbules. Melanin increases the saturation of iridescent colours seen from optimal viewing angles by absorbing back-scattered light; however, this may reduce the wide-angle brightness of these signals, contributing to a dark background appearance. We examined the nanostructure of four manakin species (Lepidothrix isidorei, L. iris, L. nattereri and L. coeruleocapilla) to identify how they produce their bright plumage colours. Feather barbs of all four species were characterized by dense and fibrous internal spongy matrices that likely increase scattering of light within the barb. The iridescent, yet pale or whitish colours of L. iris and L. nattereri feathers were produced not by periodically arranged melanosomes within barbules, but by periodic matrices of air and β-keratin within barbs. Lepidothrix iris crown feathers were able to produce a dazzling display of colours with small shifts in viewing geometry, likely because of a periodic nanostructure, a flattened barb morphology and disorder at a microstructural level. We hypothesize that iridescent plumage ornaments of male L. iris and L. nattereri are under selection to increase brightness or luminance across wide viewing angles, which may potentially increase their detectability by females during dynamic and fast-paced courtship displays in dim light environments. © 2016. Published by The Company of Biologists Ltd.

  14. Thoughts About Nursing Curricula: Dark Clouds and Bright Lights.

    Science.gov (United States)

    Turkel, Marian C; Fawcett, Jacqueline; Amankwaa, Linda; Clarke, Pamela N; Dee, Vivien; Eustace, Rosemary; Hansell, Phyllis Shanley; Jones, Dorothy A; Smith, Marlaine C; Zahourek, Rothlyn

    2018-04-01

    In this essay, several nurse scholars who are particularly concerned about the contemporary state of nursing science present their concerns about the inclusion of nursing conceptual models and theories in the curricula of nursing programs (dark clouds) and ways in which the concerns have been addressed (bright lights). This essay is the second of two essays that were catalyzed by Barrett's paper, "Again, What Is Nursing Science?" The first essay was published in the previous issue of Nursing Science Quarterly.

  15. Human Adolescent Phase Response Curves to Bright White Light.

    Science.gov (United States)

    Crowley, Stephanie J; Eastman, Charmane I

    2017-08-01

    Older adolescents are particularly vulnerable to circadian misalignment and sleep restriction, primarily due to early school start times. Light can shift the circadian system and could help attenuate circadian misalignment; however, a phase response curve (PRC) to determine the optimal time for receiving light and avoiding light is not available for adolescents. We constructed light PRCs for late pubertal to postpubertal adolescents aged 14 to 17 years. Participants completed 2 counterbalanced 5-day laboratory sessions after 8 or 9 days of scheduled sleep at home. Each session included phase assessments to measure the dim light melatonin onset (DLMO) before and after 3 days of free-running through an ultradian light-dark (wake-sleep) cycle (2 h dim [~20 lux] light, 2 h dark). In one session, intermittent bright white light (~5000 lux; four 20-min exposures) was alternated with 10 min of dim room light once per day for 3 consecutive days. The time of light varied among participants to cover the 24-h day. For each individual, the phase shift to bright light was corrected for the free-run derived from the other laboratory session with no bright light. One PRC showed phase shifts in response to light start time relative to the DLMO and another relative to home sleep. Phase delay shifts occurred around the hours corresponding to home bedtime. Phase advances occurred during the hours surrounding wake time and later in the afternoon. The transition from delays to advances occurred at the midpoint of home sleep. The adolescent PRCs presented here provide a valuable tool to time bright light in adolescents.

  16. High-brightness switchable multiwavelength remote laser in air

    Energy Technology Data Exchange (ETDEWEB)

    Yao Jinping; Cheng Ya; Xu Zhizhan [State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 (China); Zeng Bin; Li Guihua; Chu Wei; Ni Jielei; Zhang Haisu [State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 (China); Graduate School of Chinese Academy of Sciences, Beijing 100080 (China); Xu Huailiang [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012 (China); Chin, See Leang [Center for Optics, Universite Laval, Quebec City, Quebec G1V 0A6 (Canada)

    2011-11-15

    We demonstrate a harmonic-seeded switchable multiwavelength laser in air driven by intense midinfrared femtosecond laser pulses, in which population inversion occurs at an ultrafast time scale (i.e., less than {approx}200 fs) owing to direct formation of excited molecular nitrogen ions by strong-field ionization of inner-valence electrons. The bright multiwavelength laser in air opens the perspective for remote detection of multiple pollutants based on nonlinear optical spectroscopy.

  17. Very bright optical transient near the Trifid and Lagoon Nebulae

    Science.gov (United States)

    Dunsby, Peter

    2018-03-01

    Peter Dunsby (University of Cape Town) reports the detection of a very bright optical transient in the region between the Lagoon and Trifid Nebulae based on observations obtained from Cape Town on 20 March 2018, between 01:00 and 03:45 UT. The object was visible throughout the full duration of the observations and not seen when this field was observed previously (08 March 2018).

  18. Bright spots among the world’s coral reefs

    Science.gov (United States)

    Cinner, Joshua E.; Huchery, Cindy; MacNeil, M. Aaron; Graham, Nicholas A. J.; McClanahan, Tim R.; Maina, Joseph; Maire, Eva; Kittinger, John N.; Hicks, Christina C.; Mora, Camilo; Allison, Edward H.; D'Agata, Stephanie; Hoey, Andrew; Feary, David A.; Crowder, Larry; Williams, Ivor D.; Kulbicki, Michel; Vigliola, Laurent; Wantiez, Laurent; Edgar, Graham; Stuart-Smith, Rick D.; Sandin, Stuart A.; Green, Alison L.; Hardt, Marah J.; Beger, Maria; Friedlander, Alan; Campbell, Stuart J.; Holmes, Katherine E.; Wilson, Shaun K.; Brokovich, Eran; Brooks, Andrew J.; Cruz-Motta, Juan J.; Booth, David J.; Chabanet, Pascale; Gough, Charlie; Tupper, Mark; Ferse, Sebastian C. A.; Sumaila, U. Rashid; Mouillot, David

    2016-07-01

    Ongoing declines in the structure and function of the world’s coral reefs require novel approaches to sustain these ecosystems and the millions of people who depend on them. A presently unexplored approach that draws on theory and practice in human health and rural development is to systematically identify and learn from the ‘outliers’—places where ecosystems are substantially better (‘bright spots’) or worse (‘dark spots’) than expected, given the environmental conditions and socioeconomic drivers they are exposed to. Here we compile data from more than 2,500 reefs worldwide and develop a Bayesian hierarchical model to generate expectations of how standing stocks of reef fish biomass are related to 18 socioeconomic drivers and environmental conditions. We identify 15 bright spots and 35 dark spots among our global survey of coral reefs, defined as sites that have biomass levels more than two standard deviations from expectations. Importantly, bright spots are not simply comprised of remote areas with low fishing pressure; they include localities where human populations and use of ecosystem resources is high, potentially providing insights into how communities have successfully confronted strong drivers of change. Conversely, dark spots are not necessarily the sites with the lowest absolute biomass and even include some remote, uninhabited locations often considered near pristine. We surveyed local experts about social, institutional, and environmental conditions at these sites to reveal that bright spots are characterized by strong sociocultural institutions such as customary taboos and marine tenure, high levels of local engagement in management, high dependence on marine resources, and beneficial environmental conditions such as deep-water refuges. Alternatively, dark spots are characterized by intensive capture and storage technology and a recent history of environmental shocks. Our results suggest that investments in strengthening fisheries

  19. The quiet Sun brightness temperature at 408 MHz

    International Nuclear Information System (INIS)

    Avignon, Y.; Lantos, P.; Palagi, F.; Patriarchi, P.

    1975-01-01

    The flux of the radio quiet Sun and the brightness temperature at 408 MHz (73cm) are derived from measurements with the E-W Nancay interferometer and the E-W arm of the Medicina North Cross. It is shown that the lowest envelopes, which defined the radio quiet Sun, correspond to transits of extended coronal holes across the disk of the Sun. (Auth.)

  20. Brightness/darkness induction and the genesis of a contour

    Directory of Open Access Journals (Sweden)

    Sergio eRoncato

    2014-10-01

    Full Text Available Visual contours often result from the integration or interpolation of fragmented edges.The strength of the completion increases when the edges share the same contrast polarity (CP. Here we demonstrate that the appearance in the perceptual field of this integrated unit, or contour of invariant CP, is concomitant with a vivid brightness alteration of the surfaces at its opposite sides. To observe this effect requires some stratagems because the formation in the visual field of a contour of invariant CP normally engenders the formation of a second contour and then the rise of two streams of induction signals that interfere in different ways. Particular configurations have been introduced that allow us to observe the induction effects of one contour taken in isolation. I documented these effects by phenomenological observations and psychophysical measurement of the brightness alteration in relation to luminance contrast. When the edges of the same CP complete to form a contour, the background of homogeneous luminance appears to dim at one side and to brighten at the opposite side (in accord with the CP. The strength of the phenomenon is proportional to the local luminance contrast. This effect weakens or nulls when the contour of the invariant CP separates surfaces filled with different grey shades.These conflicting results stimulate a deeper exploration of the induction phenomena and their role in the computation of brightness contrast. An alternative perspective is offered to account for some brightness illusions and their relation to the phenomenal transparency. The main assumption asserts that, when in the same region induction signals of opposite CP overlap, the filling-in are blocked unless the image is stratified into different layers, one for each signal of the same polarity. Phenomenological observations document this solution by the visual system

  1. Bright to Dim Oscillatory Response of the Neurospora Circadian Oscillator

    OpenAIRE

    Gooch, Van D.; Johnson, Alicia E.; Larrondo, Luis F.; Loros, Jennifer J.; Dunlap, Jay C.

    2014-01-01

    The fungus Neurospora crassa constitutes an important model system extensively used in chronobiology. Several studies have addressed how environmental cues, such as light, can reset or synchronize a circadian system. By means of an optimized firefly luciferase reporter gene and a controllable lighting system, we show that Neurospora can display molecular circadian rhythms in dim light when cultures receive bright light prior to entering dim light conditions. We refer to this behavior as the “...

  2. Low-Surface-Brightness Galaxies: Hidden Galaxies Revealed

    Science.gov (United States)

    Bothun, G.; Impey, C.; McGaugh, S.

    1997-07-01

    In twenty years, low surface brightness (LSB) galaxies have evolved from being an idiosyncratic notion to being one of the major baryonic repositories in the Universe. The story of their discovery and the characterization of their properties is told here. Their recovery from the noise of the night sky background is a strong testament to the severity of surface brightness selection effects. LSB galaxies have a number of remarkable properties which distinguish them from the more familiar Hubble Sequence of spirals. The two most important are 1) they evolve at a significantly slower rate and may well experience star formation outside of the molecular cloud environment, 2) they are embedded in dark matter halos which are of lower density and more extended than the halos around high surface brightness (HSB) disk galaxies. Compared to HSB disks, LSB disks are strongly dark matter dominated at all radii and show a systematic increase in $M/L$ with decreasing central surface brightness. In addition, the recognition that large numbers of LSB galaxies actually exist has changed the form of the galaxy luminosity function and has clearly increased the space density of galaxies at z =0. Recent CCD surveys have uncovered a population of red LSB disks that may be related to the excess of faint blue galaxies detected at moderate redshifts. LSB galaxies offer us a new window into galaxy evolution and formation which is every bit as important as those processes which have produced easy to detect galaxies. Indeed, the apparent youth of some LSB galaxies suggest that galaxy formation is a greatly extended process. While the discovery of LSB galaxies have lead to new insights, it remains unwise to presume that we now have a representative sample which encompasses all galaxy types and forms. (SECTION: Invited Review Paper)

  3. Near-Real-Time DMSP SSM/I-SSMIS Daily Polar Gridded Brightness Temperatures

    Data.gov (United States)

    National Aeronautics and Space Administration — The Near-Real-Time DMSP SSM/I-SSMIS Daily Polar Gridded Brightness Temperature product provides near-real-time brightness temperatures for both the Northern and...

  4. Blue and green emitting Ce3+ and Tb3+ codoped Gd2O3 nanophosphors

    International Nuclear Information System (INIS)

    Loitongbam, Romeo Singh; Singh, W. Rameshwor

    2013-01-01

    Tb 3+ doped Gd 2 O 3 nanoparticles of 4-10 nm in size were synthesized from nitrate precursors by urea hydrolysis method in ethylene glycol medium at low temperature of 140 ℃. Characteristic Tb 3+ ion green emission corresponding to 5 D 4 ’! 7 F J was observed to be very strong, which further increases with heat treatment temperature. Characteristic blue color emission of Ce 3+ ion transitions for 5 d’! 2 F 7/2 and 2 F 5/2 (at 422 nm and 485 nm respectively) was found to be very strong in as-synthesized Ce 0.06 Tb 0.14 Gd 0.8 O 3 nanoparticles. However, its luminescence intensity decreases with increase in heating temperature or increase in the particle size/crystallinity, whereas there was a weak emission peak of Tb 3+ ion at 545 nm. The polycrystalline nature of as-prepared sample change to highly crystalline state when heated at elevated temperature (900 ℃). (author)

  5. Optical properties of wavelength-tunable green-emitting color conversion glass ceramics

    Science.gov (United States)

    Li, Yang; Hu, Li-Li; Yang, Bo-Bo; Shi, Ming-Ming; Zou, Jun

    2017-12-01

    Not Available Project supported by the National Natural Science Foundation of China (Grant No. 51302171), the Science and Technology Commission of Shanghai Municipality, China (Grant No. 14500503300), and the Natural Science Foundation of Shanghai, China (Grant No. 12ZR1430900).

  6. On correct evaluation techniques of brightness enhancement effect measurement data

    Science.gov (United States)

    Kukačka, Leoš; Dupuis, Pascal; Motomura, Hideki; Rozkovec, Jiří; Kolář, Milan; Zissis, Georges; Jinno, Masafumi

    2017-11-01

    This paper aims to establish confidence intervals of the quantification of brightness enhancement effects resulting from the use of pulsing bright light. It is found that the methods used so far may yield significant bias in the published results, overestimating or underestimating the enhancement effect. The authors propose to use a linear algebra method called the total least squares. Upon an example dataset, it is shown that this method does not yield biased results. The statistical significance of the results is also computed. It is concluded over an observation set that the currently used linear algebra methods present many patterns of noise sensitivity. Changing algorithm details leads to inconsistent results. It is thus recommended to use the method with the lowest noise sensitivity. Moreover, it is shown that this method also permits one to obtain an estimate of the confidence interval. This paper neither aims to publish results about a particular experiment nor to draw any particular conclusion about existence or nonexistence of the brightness enhancement effect.

  7. Bright Soil Churned by Spirit's Sol 1861 Drive

    Science.gov (United States)

    2009-01-01

    NASA's Mars Exploration Rover Spirit drove 22.7 meters (74 feet) toward the southwest on the 1,861st Martian day, or sol, of Spirit's mission on Mars (March 28, 2009). After the drive, the rover took this image with its front hazard-avoidance camera, looking back at the tracks from the drive. As usual since losing the use of its right-front wheel in 2006, Spirit drove backwards. The immobile right-front wheel churned up a long stripe of bright soil during this drive. Where Spirit has found such bright soil in the past, subsequent analysis of the composition found concentrations of sulfur or silica that testified to past action of water at the site. When members of the rover team saw the large quantity of bright soil exposed by the Sol 1861 drive, they quickly laid plans to investigate the composition with Spirit's alpha particle X-ray spectrometer. The Sol 1861 drive took the rover past the northwest corner of the low plateau called 'Home Plate,' making progress on a route around the western side of Home Plate. The edge of Home Plate forms the horizon on the right side of this image. Husband Hill is on the horizon on the left side. For scale, the parallel rover wheel tracks are about 1 meter (40 inches) apart. The rover's hazard-avoidance cameras take 'fisheye' wide-angle images.

  8. Inversion methods for analysis of neutron brightness measurements in tokamaks

    International Nuclear Information System (INIS)

    Gorini, G.; Gottardi, N.

    1990-02-01

    The problem of determining neutron emissivity from neutron brightness measurements in magnetic fusion plasmas is addressed. In the case of two-dimensional measurements with two orthogonal cameras, a complete, tomographic analysis of the data can in principle be performed. The results depend critically on the accuracy of the measurements and alternative solutions can be sought under the assumption of a known emissivity topology (Generalized Abel Inversion). In this work, neutron brightness data from the JET tokamak have been studied with both methods. We find that with the present experimental uncertainty (levels 10-20%) the Abel inversion method works best, while two-dimensional information cannot in general be deduced. This is confirmed by studies of the error propagation in the inversion using artificial data, which are also presented here. An important application of emissivity profile information is the determination of the plasma deuterium temperature profile, T D (R). Results are presented here from the analysis of JET data and the errors in T D (R) are discussed in some detail. It is found that, for typical JET plasma conditions, the dominant source of uncertainty arises from the high plasma impurity level and the fact that it is poorly known; these problems can be expected to be remedied and neutron brightness measurements would be expected to be very effective (especially in high density plasmas) as a T D (R) diagnostics. (author)

  9. SMOS brightness temperature assimilation into the Community Land Model

    Directory of Open Access Journals (Sweden)

    D. Rains

    2017-11-01

    Full Text Available SMOS (Soil Moisture and Ocean Salinity mission brightness temperatures at a single incident angle are assimilated into the Community Land Model (CLM across Australia to improve soil moisture simulations. Therefore, the data assimilation system DasPy is coupled to the local ensemble transform Kalman filter (LETKF as well as to the Community Microwave Emission Model (CMEM. Brightness temperature climatologies are precomputed to enable the assimilation of brightness temperature anomalies, making use of 6 years of SMOS data (2010–2015. Mean correlation R with in situ measurements increases moderately from 0.61 to 0.68 (11 % for upper soil layers if the root zone is included in the updates. A reduced improvement of 5 % is achieved if the assimilation is restricted to the upper soil layers. Root-zone simulations improve by 7 % when updating both the top layers and root zone, and by 4 % when only updating the top layers. Mean increments and increment standard deviations are compared for the experiments. The long-term assimilation impact is analysed by looking at a set of quantiles computed for soil moisture at each grid cell. Within hydrological monitoring systems, extreme dry or wet conditions are often defined via their relative occurrence, adding great importance to assimilation-induced quantile changes. Although still being limited now, longer L-band radiometer time series will become available and make model output improved by assimilating such data that are more usable for extreme event statistics.

  10. Three Bright X-ray Sources in NGC 1313

    Science.gov (United States)

    Colbert, E.; Petre, R.; Schlegel, E.

    1992-12-01

    Three bright X-ray sources were detected in a recent (April/May 1991) ROSAT PSPC observation of the nearby (D ~ 4.5 Mpc) face--on barred spiral galaxy NGC 1313. Two of the sources were at positions coincident with X-ray sources detected by Fabbiano & Trinchieri (ApJ 315, 1987) in a previous (Jan 1980) Einstein IPC observation. The position of the brightest Einstein source is near the center of NGC 1313, and the second Einstein source is ~ 7' south of the ``nuclear'' source, in the outskirts of the spiral arms. A third bright X-ray source was detected in the ROSAT observation ~ 7' southwest of the ``nuclear'' source. We present X-ray spectra and X-ray images for the three bright sources found in the ROSAT observation of NGC 1313, and compare with previous Einstein results. Spectral analysis of these sources require them to have very large soft X-ray luminosities ( ~ 10(40) erg s(-1) ) when compared with typical X-ray sources in our Galaxy. Feasible explanations for the X-ray emission are presented. The third X-ray source is positively identified with the recently discovered (Ryder et. al., ApJ 1992) peculiar type-II supernova 1978K.

  11. Transgenic nude mouse with green fluorescent protein expression-based human glioblastoma multiforme animal model with EGFR expression and invasiveness.

    Science.gov (United States)

    Tan, Guo-Wei; Lan, Fo-Lin; Gao, Jian-Guo; Jiang, Cai-Mou; Zhang, Yi; Huang, Xiao-Hong; Ma, Yue-Hong; Shao, He-Dui; He, Xue-Yang; Chen, Jin-Long; Long, Jian-Wu; Xiao, Hui-Sheng; Guo, Zhi-Tong; Diao, Yi

    2012-08-01

    Previously, we developed an orthotopic xenograft model of human glioblastoma multiforme (GBM) with high EGFR expression and invasiveness in Balb/c nu/nu nude mice. Now we also developed the same orthotopic xenograft model in transgenic nude mice with green fluorescent protein (GFP) expression. The present orthotopic xenografts labeled by phycoerythrin fluorescing red showed high EGFR expression profile, and invasive behavior under a bright green-red dual-color fluorescence background. A striking advantage in the present human GBM model is that the change of tumor growth can be observed visually instead of sacrificing animals in our further antitumor therapy studies.

  12. Chip-scale fluorescence microscope based on a silo-filter complementary metal-oxide semiconductor image sensor.

    Science.gov (United States)

    Ah Lee, Seung; Ou, Xiaoze; Lee, J Eugene; Yang, Changhuei

    2013-06-01

    We demonstrate a silo-filter (SF) complementary metal-oxide semiconductor (CMOS) image sensor for a chip-scale fluorescence microscope. The extruded pixel design with metal walls between neighboring pixels guides fluorescence emission through the thick absorptive filter to the photodiode of a pixel. Our prototype device achieves 13 μm resolution over a wide field of view (4.8 mm × 4.4 mm). We demonstrate bright-field and fluorescence longitudinal imaging of living cells in a compact, low-cost configuration.

  13. Nanosecond fluorescence spectroscopy

    International Nuclear Information System (INIS)

    Leskovar, B.

    1985-03-01

    This article is a summary of a short course lecture given in conjunction with the 1984 Nuclear Science Symposium. Measuring systems for nanosecond fluorescence spectroscopy using single-photon counting techniques are presented. These involve systems based on relaxation-type spark gap light pulser and synchronously pumped mode-locked dye lasers. Furthermore, typical characteristics and optimization of operating conditions of the critical components responsible for the system time resolution are discussed. A short comparison of the most important deconvolution methods for numerical analysis of experimental data is given particularly with respect to the signal-to-noise ratio of the fluorescence signal. 22 refs., 8 figs

  14. Fluorescence uranium determination

    International Nuclear Information System (INIS)

    Fernandez Cellini, R.; Crus Castillo, F. de la; Barrera Pinero, R.

    1960-01-01

    An equipment for analysis of uranium by fluorescence was developed in order to determine it at such a low concentration that it can not be determined by the most sensible analytical methods. this new fluorimeter was adapted to measure the fluorescence emitted by the phosphorus sodium fluoride-sodium carbonate-potasium carbonate-uranyl, being excited by ultraviolet light of 3,650 A the intensity of the light emitted was measure with a photomultiplicator RCA 5819 and the adequate electronic equipment. (Author) 19 refs

  15. Controllable ultra-narrow fluorescence and six-wave mixing under double electromagnetically induced transparency

    International Nuclear Information System (INIS)

    Wang, Z G; Zhang, Z Y; Che, J L; Zhang, Y Z; Li, C B; Zheng, H B; Zhang, Y P

    2013-01-01

    We report the first observation of six-wave mixing (SWM) and fluorescence signals in an electromagnetically induced transparency (EIT) window. Several remarkable advantages are described. First, multiple bright and dark states are simultaneously observed due to enhancement or suppression of the SWM signal. Second, ultra-narrow fluorescence, much narrower than the EIT window, is experimentally obtained. Third, the ultra-narrow fluorescence can also generate Autler–Townes splitting on scanning the coupling beam. Fourth, a double-peak EIT window is obtained using the nest-dressing scheme. Such studies concerning SWM and fluorescence have applications in optical switching, multi-channel communication and narrowband and long-range quantum communication. (letter)

  16. Distributed fluorescent optical fiber proximity sensor: Towards a proof of concept

    Science.gov (United States)

    Gălătuș, Ramona; Faragó, Paul; Miluski, Piotr; Valles, Juan-Antonio

    2018-06-01

    Fluorescent fibers are optical fibers which emit light as a response to an incident phenomenon, usually an incident light. Operation depends on the doping dyes, which determine specific fluorescence and optical characteristics useful in the development of optical sensors. In this work we propose a low-cost distributed proximity sensor implemented using a red fluorescent fiber, to provide a security option for a surface plasmon resonance system. Operation of the proposed sensor relies on having the incident illumination intensity varied by the presence or absence of an obstacle in the vicinity of the sensing element. This will influence the radiated fluorescence accordingly. The proposed setup for the implementation of the optical proximity sensor assumes having a high brightness LED deployed for axial fiber illumination and a blue LED for side illumination. Electronic processing then accounts for gain and digitization. Measurement results of the prototype validate the proposed concept.

  17. The Physics and Applications of High Brightness Electron Beams

    Science.gov (United States)

    Palumbo, Luigi; Rosenzweig, J.; Serafini, Luca

    2007-09-01

    Plenary sessions. RF deflector based sub-Ps beam diagnostics: application to FEL and advanced accelerators / D. Alesini. Production of fermtosecond pulses and micron beam spots for high brightness electron beam applications / S.G. Anderson ... [et al.]. Wakefields of sub-picosecond electron bunches / K.L.F. Bane. Diamond secondary emitter / I. Ben-Zvi ... [et al.]. Parametric optimization for an X-ray free electron laser with a laser wiggler / R. Bonifacio, N. Piovella and M.M. Cola. Needle cathodes for high-brightness beams / C.H. Boulware ... [et al.]. Non linear evolution of short pulses in FEL cascaded undulators and the FEL harmonic cascade / L. Giannessi and P. Musumeci. High brightness laser induced multi-meV electron/proton sources / D. Giulietti ... [et al.]. Emittance limitation of a conditioned beam in a strong focusing FEL undulator / Z. Huang, G. Stupakov and S. Reiche. Scaled models: space-charge dominated electron storage rings / R.A. Kishek ... [et al.]. High brightness beam applications: energy recovered linacs / G.A. Krafft. Maximizing brightness in photoinjectors / C. Limborg-Deprey and H. Tomizawa. Ultracold electron sources / O.J. Luiten ... [et al.]. Scaling laws of structure-based optical accelerators / A. Mizrahi, V. Karagodsky and L. Schächter. High brightness beams-applications to free-electron lasers / S. Reiche. Conception of photo-injectors for the CTF3 experiment / R. Roux. Superconducting RF photoinjectors: an overview / J. Sekutowicz. Status and perspectives of photo injector developments for high brightness beams / F. Stephan. Results from the UCLA/FNLP underdense plasma lens experiment / M.C. Thompson ... [et al.]. Medical application of multi-beam compton scattering monochromatic tunable hard X-ray source / M. Uesaka ... [et al.]. Design of a 2 kA, 30 fs RF-photoinjector for waterbag compression / S.B. Van Der Geer, O.J. Luiten and M.J. De Loos. Proposal for a high-brightness pulsed electron source / M. Zolotorev ... [et al

  18. Fabrication of L-cysteine-capped CdTe quantum dots based ratiometric fluorescence nanosensor for onsite visual determination of trace TNT explosive

    Energy Technology Data Exchange (ETDEWEB)

    Qian, Jing; Hua, Mengjuan [School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013 (China); Wang, Chengquan [Changzhou College of Information Technology, Changzhou 213164 (China); Wang, Kan; Liu, Qian; Hao, Nan [School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013 (China); Wang, Kun, E-mail: wangkun@ujs.edu.cn [School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013 (China)

    2016-11-23

    New strategies for onsite determination of trace 2,4,6-trinitrotoluene (TNT) explosives have become a research hotspot for homeland security needs against terrorism and environmental concerns. Herein, we designed a ratiometric fluorescence nanohybrid comprising 3-mercaptopropionic acid-capped green-emitting CdTe quantum dots (gQDs) encapsulated into SiO{sub 2} sphere and L-cysteine (Lcys)-capped red-emitting CdTe QDs (rQDs) conjugated onto SiO{sub 2} surface. The surface Lcys can be used as not only the stabilizer of the rQDs but also the primary amine provider which can react with TNT to form Meisenheimer complexes. Without any additional surface modification procedure, the fluorescence of rQDs equipped with Lcys was selectively quenched by TNT because electrons of the rQDs transferred to TNT molecules due to the formation of Meisenheimer complexes. Meanwhile, the embedded gQDs always remained constant. Upon exposure to increasing amounts of TNT, the fluorescence of rQDs could be gradually quenched and consequently the logarithm of the dual emission intensity ratios exhibited a good linear negative correlation with TNT concentration over a range of 10 nM–8 μM with a low detection limit of 3.3 nM. One can perform onsite visual determination of TNT with high resolution because the ratiometric fluorescence nanosensing system exhibited obvious fluorescence color changes. This sensing strategy has been successfully applied in real samples and already integrated in a filter paper-based assay, which enables potential fields use application featuring easy handling and cost-effectiveness. - Highlights: • A facile strategy for preparing Lcys-capped rQDs based hybrid spheres was reported. • Lcys serves as the stabilizer of rQDs and primary amine provider to react with TNT. • One can perform onsite visual determination of TNT by using such probe. • The nanosensor exhibited a wide linear range and a low detection limit. • This sensing strategy can be fully

  19. HIGH ENERGY, HIGH BRIGHTNESS X-RAYS PRODUCED BY COMPTON BACKSCATTERING AT THE LIVERMORE PLEIADES FACILITY

    International Nuclear Information System (INIS)

    Tremaine, A M; Anderson, S G; Betts, S; Crane, J; Gibson, D J; Hartemann, F V; Jacob, J S; Frigola, P; Lim, J; Rosenzweig, J; Travish, G

    2005-01-01

    PLEIADES (Picosecond Laser Electron Interaction for the Dynamic Evaluation of Structures) produces tunable 30-140 keV x-rays with 0.3-5 ps pulse lengths and up to 10 7 photons/pulse by colliding a high brightness electron beam with a high power laser. The electron beam is created by an rf photo-injector system, accelerated by a 120 MeV linac, and focused to 20 (micro)m with novel permanent magnet quadrupoles. To produce Compton back scattered x-rays, the electron bunch is overlapped with a Ti:Sapphire laser that delivers 500 mJ, 100 fs, pulses to the interaction point. K-edge radiography at 115 keV on Uranium has verified the angle correlated energy spectrum inherent in Compton scattering and high-energy tunability of the Livermore source. Current upgrades to the facility will allow laser pumping of targets synchronized to the x-ray source enabling dynamic diffraction and time-resolved studies of high Z materials. Near future plans include extending the radiation energies to >400 keV, allowing for nuclear fluorescence studies of materials

  20. Platinum plasmonic nanostructure arrays for massively parallel single-molecule detection based on enhanced fluorescence measurements

    International Nuclear Information System (INIS)

    Saito, Toshiro; Takahashi, Satoshi; Obara, Takayuki; Itabashi, Naoshi; Imai, Kazumichi

    2011-01-01

    We fabricated platinum bowtie nanostructure arrays producing fluorescence enhancement and evaluated their performance using two-photon photoluminescence and single-molecule fluorescence measurements. A comprehensive selection of suitable materials was explored by electromagnetic simulation and Pt was chosen as the plasmonic material for visible light excitation near 500 nm, which is preferable for multicolor dye-labeling applications like DNA sequencing. The observation of bright photoluminescence (λ = 500-600 nm) from each Pt nanostructure, induced by irradiation at 800 nm with a femtosecond laser pulse, clearly indicates that a highly enhanced local field is created near the Pt nanostructure. The attachment of a single dye molecule was attempted between the Pt triangles of each nanostructure by using selective immobilization chemistry. The fluorescence intensities of the single dye molecule localized on the nanostructures were measured. A highly enhanced fluorescence, which was increased by a factor of 30, was observed. The two-photon photoluminescence intensity and fluorescence intensity showed qualitatively consistent gap size dependence. However, the average fluorescence enhancement factor was rather repressed even in the nanostructure with the smallest gap size compared to the large growth of photoluminescence. The variation of the position of the dye molecule attached to the nanostructure may influence the wide distribution of the fluorescence enhancement factor and cause the rather small average value of the fluorescence enhancement factor.

  1. Monitoring by fluorescence measurements

    International Nuclear Information System (INIS)

    Malcolme-Lawes, D.J.; Gifford, L.A.

    1981-01-01

    A fluorimetric detector is described in which the fluorescence excitation source may be 3 H, 14 C, 35 S, 147 Pm or 63 Ni. Such a detector can be adapted for use with flowing liquid systems especially liquid chromatography systems. (U.K.)

  2. Fluorescence lifetime based bioassays

    Science.gov (United States)

    Meyer-Almes, Franz-Josef

    2017-12-01

    Fluorescence lifetime (FLT) is a robust intrinsic property and material constant of fluorescent matter. Measuring this important physical indicator has evolved from a laboratory curiosity to a powerful and established technique for a variety of applications in drug discovery, medical diagnostics and basic biological research. This distinct trend was mainly driven by improved and meanwhile affordable laser and detection instrumentation on the one hand, and the development of suitable FLT probes and biological assays on the other. In this process two essential working approaches emerged. The first one is primarily focused on high throughput applications employing biochemical in vitro assays with no requirement for high spatial resolution. The second even more dynamic trend is the significant expansion of assay methods combining highly time and spatially resolved fluorescence data by fluorescence lifetime imaging. The latter approach is currently pursued to enable not only the investigation of immortal tumor cell lines, but also specific tissues or even organs in living animals. This review tries to give an actual overview about the current status of FLT based bioassays and the wide range of application opportunities in biomedical and life science areas. In addition, future trends of FLT technologies will be discussed.

  3. Fluorescent Lamp Replacement Study

    Science.gov (United States)

    2017-07-01

    not be cited for purposes of advertisement. DISPOSITION INSTRUCTIONS: Destroy this document when no longer needed. Do not return to the... recycling , and can be disposed safely in a landfill. (2) LEDs offer reduced maintenance costs and fewer bulb replacements, significantly reducing... recycling . Several fixtures, ballasts and energy efficient fluorescent bulbs that were determined to be in pristine condition were returned to ATC

  4. Selective turn-on fluorescent probes for imaging hydrogen sulfide in living cells.

    Science.gov (United States)

    Montoya, Leticia A; Pluth, Michael D

    2012-05-16

    Hydrogen sulfide (H(2)S) is an important biological messenger but few biologically-compatible methods are available for its detection. Here we report two bright fluorescent probes that are selective for H(2)S over cysteine, glutathione and other reactive sulfur, nitrogen, and oxygen species. Both probes are demonstrated to detect H(2)S in live cells. This journal is © The Royal Society of Chemistry 2012

  5. Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy

    Czech Academy of Sciences Publication Activity Database

    Macháň, Radek; Kapusta, Peter; Hof, Martin

    Roč. 406 , č. 20 (2014), s. 4797-4813 ISSN 1618-2642 R&D Projects: GA ČR GBP208/12/G016 Institutional support: RVO:61388955 Keywords : Filtered fluorescence correlation spectroscopy * Fluorescence lifetime correlation spectroscopy * Fluorescence spectral correlation spectroscopy Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.436, year: 2014

  6. Label-free detection of cellular drug responses by high-throughput bright-field imaging and machine learning.

    Science.gov (United States)

    Kobayashi, Hirofumi; Lei, Cheng; Wu, Yi; Mao, Ailin; Jiang, Yiyue; Guo, Baoshan; Ozeki, Yasuyuki; Goda, Keisuke

    2017-09-29

    In the last decade, high-content screening based on multivariate single-cell imaging has been proven effective in drug discovery to evaluate drug-induced phenotypic variations. Unfortunately, this method inherently requires fluorescent labeling which has several drawbacks. Here we present a label-free method for evaluating cellular drug responses only by high-throughput bright-field imaging with the aid of machine learning algorithms. Specifically, we performed high-throughput bright-field imaging of numerous drug-treated and -untreated cells (N = ~240,000) by optofluidic time-stretch microscopy with high throughput up to 10,000 cells/s and applied machine learning to the cell images to identify their morphological variations which are too subtle for human eyes to detect. Consequently, we achieved a high accuracy of 92% in distinguishing drug-treated and -untreated cells without the need for labeling. Furthermore, we also demonstrated that dose-dependent, drug-induced morphological change from different experiments can be inferred from the classification accuracy of a single classification model. Our work lays the groundwork for label-free drug screening in pharmaceutical science and industry.

  7. Who's who in fluorescence 2008

    CERN Document Server

    Geddes, Chris D

    2008-01-01

    The Journal of Fluorescence's sixth Who's Who directory publishes the names, contact details, specialty keywords, and a brief description of scientists employing fluorescence methodology and instrumentation in their working lives. This is a unique reference.

  8. A new strategy for synthesizing AgInS2 quantum dots emitting brightly in near-infrared window for in vivo imaging

    DEFF Research Database (Denmark)

    Tan, Lianjiang; Liu, Shuiping; Li, Xiaoqiang

    2015-01-01

    A new strategy for fabricating water-dispersible AgInS2 quantum dots (QDs) with bright near-infrared (NIR) emission is demonstrated. A type of multidentate polymer (MDP) was synthesized and utilized as a compact capping ligand for the AgInS2 QDs. Using silver nitrate, indium acetate and sulfur-hy...... cytotoxicity. Nude mice photoluminescence imaging shows that the MDP-capping AgInS2 QDs can be well applied to in vivo imaging. These readily prepared NIR fluorescent nanocrystals have huge potential for biomedical applications....

  9. Variation reduction of brightness and pH of pulp sent to a paper mill

    Directory of Open Access Journals (Sweden)

    Napassavong Rojanarowan

    2015-03-01

    Full Text Available The variance of the brightness of pulp sent to the paper mill during the changing period of dry pulp grades affects the chemical control in the paper mill. This research aims to determine the mixing formula of pulp with different brightness from the EOP and D1 stages to handle this variation issue. This research uses response surface design with Central Composite Design type, regression technique and optimization technique to find the optimal setting of the mixing formula for each of the seven brightness levels to obtain the target brightness of 86% and the pH of 5.25. The mixing formulas are determined by the pulp mixing percentage and the sulfuric acid consumption. The experimental results reveal that when using higher EOP mixing ratio, the brightness decreases and the pH increases. Regarding the effect of the sulfuric acid, increasing the sulfuric acid makes the brightness and the pH decrease. After implementing the optimal formula in the production line, the mean of pulp brightness is closer to the target compared with the brightness before improvement and the brightness variation decreases without affecting the quality of other pulp grades, average of brightness decreased from 87.4% to 86.3% and standard deviation of brightness decreased from 1.09 to 0.46.

  10. Flux and brightness calculations for various synchrotron radiation sources

    International Nuclear Information System (INIS)

    Weber, J.M.; Hulbert, S.L.

    1991-11-01

    Synchrotron radiation (SR) storage rings are powerful scientific and technological tools. The first generation of storage rings in the US., e.g., SURF (Washington, D.C.), Tantalus (Wisconsin), SSRL (Stanford), and CHESS (Cornell), revolutionized VUV, soft X-ray, and hard X-ray science. The second (present) generation of storage rings, e.g. the NSLS VUV and XRAY rings and Aladdin (Wisconsin), have sustained the revolution by providing higher stored currents and up to a factor of ten smaller electron beam sizes than the first generation sources. This has made possible a large number of experiments that could not performed using first generation sources. In addition, the NSLS XRAY ring design optimizes the performance of wigglers (high field periodic magnetic insertion devices). The third generation storage rings, e.g. ALS (Berkeley) and APS (Argonne), are being designed to optimize the performance of undulators (low field periodic magnetic insertion devices). These extremely high brightness sources will further revolutionize x-ray science by providing diffraction-limited x-ray beams. The output of undulators and wigglers is distinct from that of bending magnets in magnitude, spectral shape, and in spatial and angular size. Using published equations, we have developed computer programs to calculate the flux, central intensity, and brightness output bending magnets and selected wigglers and undulators of the NSLS VUV and XRAY rings, the Advanced Light Source (ALS), and the Advanced Photon Source (APS). Following is a summary of the equations used, the graphs and data produced, and the computer codes written. These codes, written in the C programming language, can be used to calculate the flux, central intensity, and brightness curves for bending magnets and insertion devices on any storage ring

  11. Low surface brightness galaxies in the Fornax Cluster: automated galaxy surface photometry

    International Nuclear Information System (INIS)

    Davies, J.I.; Phillipps, S.; Disney, M.J.

    1988-01-01

    A sample is presented of low surface brightness galaxies (with extrapolated central surface brightness fainter than 22.0 Bμ) in the Fornax Cluster region which has been measured by the APM machine. Photometric parameters, namely profile shape, scale length, central brightness and total magnitude, are derived for the sample galaxies and correlations between the parameters of low surface brightness dwarf galaxies are discussed, with particular reference to the selection limits. Contrary to previous authors we find no evidence for a luminosity-surface brightness correlation in the sense of lower surface brightness galaxies having lower luminosities and scale sizes. In fact, the present data suggest that it is the galaxies with the largest scale lengths which are more likely to be of very low surface brightness. In addition, the larger scale length galaxies occur preferentially towards the centre of the Cluster. (author)

  12. Synthesis and Fluorescence Spectra of Triazolylcoumarin Fluorescent Dyes

    Institute of Scientific and Technical Information of China (English)

    PENG Xian-fu; LI Hong-qi

    2009-01-01

    Much attention is devoted to fluorescent dyes especially those with potential in versatile applications. Reactions under "click" conditions between nonfluorescent 3 - azidocoumarins and terminal alkynes produced 3 -(1, 2, 3- triazol- 1 - yl)cournarins, a novel type of fluorescent dyes with intense fluorescence. The structures of the new coumarins were characterized by 1H NMR, MS, and IR spectra. Fluorescence spectra measurement demonstrated excellent fluorescence performance of the triazolylcoumarins and this click reaction is a promising candidate for bioconjugation and bioimaging applications since both azide and alkynes are quite inert to biological systems.

  13. Bright, dark and singular optical solitons in a cascaded system

    International Nuclear Information System (INIS)

    Zhou, Qin; Zhu, Qiuping; Yu, Hua; Liu, Yaxian; Wei, Chun; Yao, Ping; Bhrawy, Ali H; Biswas, Anjan

    2015-01-01

    This work studies nonlinear dynamics of optical solitons in a cascaded system with Kerr law nonlinearity and spatio-temporal dispersion. The mathematical model that describes the propagation of optical solitons through a cascaded system is given by the vector-coupled nonlinear Schrödinger equation. It is investigated analytically using three integration algorithms. The Jacobian elliptic equation expansion method, Bernoulli equation expansion approach and Riccati equation expansion scheme are the integration tools of this model that are recruited to extract singular, bright and dark solitons. The restrictions that need to hold for the existence of these solitons are derived. (paper)

  14. The bright optical afterglow of the long GRB 001007

    DEFF Research Database (Denmark)

    Ceron, J.M.C.; Castro-Tirado, A.J.; Gorosabel, J.

    2002-01-01

    We present optical follow up observations of the long GRB 001007 between 6.14 hours and similar to468 days after the event. An unusually bright optical afterglow (OA) was seen to decline following a steep power law decay with index alpha = -2.03 +/- 0.11, possibly indicating a break in the light...... curve at t - t(0) hours after the gamma ray event provide tentative (1.2σ) evidence for a break in the optical light curve. The spectral index β of the OA yields -1.24 +/- 0.57. These values may be explained both...

  15. Bright X-ray transient in the LMC

    Science.gov (United States)

    Saxton, R.; Read, A. M.; Li, D. Y.

    2018-01-01

    We report a bright X-ray transient in the LMC from an XMM-Newton slew made on 5th January 2018. The source, XMMSL2 J053629.4-675940, had a soft X-ray (0.2-2 keV) count rate in the EPIC-pn detector, medium filter of 1.82+/-0.56 c/s, equivalent to a flux Fx=2.3+/-0.7E-12 ergs/s/cm2 for a nominal spectrum of a power-law of slope 2 absorbed by a column NH=3E20 cm^-2.

  16. Technological Challenges for High-Brightness Photo-Injectors

    CERN Multimedia

    Suberlucq, Guy

    2004-01-01

    Many applications, from linear colliders to free-electron lasers, passing through light sources and many other electron sources, require high brightness electron beams, usually produced by photo-injectors. Because certain parameters of these applications differ by several orders of magnitude, various solutions were implemented for the design and construction of the three main parts of the photo-injectors: lasers, photocathodes and guns. This paper summarizes the different requirements, how they lead to technological challenges and how R&D programs try to overcome these challenges. Some examples of state-of-the-art parts are presented.

  17. Bright photoactivatable fluorophores for single-molecule imaging.

    Science.gov (United States)

    Grimm, Jonathan B; English, Brian P; Choi, Heejun; Muthusamy, Anand K; Mehl, Brian P; Dong, Peng; Brown, Timothy A; Lippincott-Schwartz, Jennifer; Liu, Zhe; Lionnet, Timothée; Lavis, Luke D

    2016-12-01

    Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

  18. Completion of the brightness upgrade of the ALS

    International Nuclear Information System (INIS)

    Steier, C; Madur, A; Bailey, B; Berg, K; Biocca, A; Black, A; Casey, P; Colomb, D; Gunion, B; Li, N; Marks, S; Nishimura, H; Pappas, C; Petermann, K; Portmann, G; Prestemon, S; Rawlins, A; Robin, D; Rossi, S; Scarvie, T

    2014-01-01

    The Advanced Light Source (ALS) at Berkeley Lab remains one of the brightest sources for soft x-rays worldwide. A multiyear upgrade of the ALS is underway, which includes new and replacement x-ray beamlines, a replacement of many of the original insertion devices and many upgrades to the accelerator. The accelerator upgrade that affects the ALS performance most directly is the ALS brightness upgrade [1], which reduces the horizontal emittance from 6.3 to 2.0 nm (2.5 nm effective). Magnets for this upgrade were installed in late 2012 and early 2013 followed by user operation with the reduced emittance.

  19. Generation of high brightness ion beam from insulated anode PED

    International Nuclear Information System (INIS)

    Matsukawa, Yoshinobu

    1988-01-01

    Generation and focusing of a high density ion beam with high brightness from a organic center part of anode of a PED was reported previously. Mass, charge and energy distribution of this beam were analyzed. Three kind of anode were tried. Many highly ionized medium mass ions (up to C 4+ , O 6+ ) accelarated to several times of voltage difference between anode and cathode were observed. In the case of all insulator anode the current carried by the medium mass ions is about half of that carried by protons. (author)

  20. FACT — LONGTERM MONITORING OF BRIGHT TeV BLAZARS

    Directory of Open Access Journals (Sweden)

    Katja Meier

    2014-06-01

    Full Text Available The First G-APD Cherenkov Telescope (FACT, located on the Canary Island of La Palma, has been taking data since October 2011. FACT has been optimized for longterm monitoring of bright TeV blazars, to study their variability time scales and flare probability. G-APD photo-sensors allow for observations even under strong moonlight conditions, and the telescope can be operated remotely. The monitoring strategy of FACT is discussed and preliminary results of the flare of Mrk501 in June 2012 are shown.

  1. Automatic brightness control algorithms and their effect on fluoroscopic imaging

    International Nuclear Information System (INIS)

    Quinn, P.W.; Gagne, R.M.

    1989-01-01

    This paper reports a computer model used to investigate the effect on dose and image quality of three automatic brightness control (ABC) algorithms used in the imaging of barium during general-purpose fluoroscopy. A model incorporating all aspects of image formation - i.e., x- ray production, phantom attenuation, and energy absorption in the CSI phosphor - was driven according to each ABC algorithm as a function of patient thickness. The energy absorbed in the phosphor was kept constant, while the changes in exposure, integral dose, organ dose, and contrast were monitored

  2. Fluorescence spectroscopy of dental calculus

    International Nuclear Information System (INIS)

    Bakhmutov, D; Gonchukov, S; Sukhinina, A

    2010-01-01

    The aim of the present study was to investigate the fluorescence properties of dental calculus in comparison with the properties of adjacent unaffected tooth structure using both lasers and LEDs in the UV-visible range for fluorescence excitation. The influence of calculus color on the informative signal is demonstrated. The optimal spectral bands of excitation and registration of the fluorescence are determined

  3. Fluorescence spectroscopy of dental calculus

    Science.gov (United States)

    Bakhmutov, D.; Gonchukov, S.; Sukhinina, A.

    2010-05-01

    The aim of the present study was to investigate the fluorescence properties of dental calculus in comparison with the properties of adjacent unaffected tooth structure using both lasers and LEDs in the UV-visible range for fluorescence excitation. The influence of calculus color on the informative signal is demonstrated. The optimal spectral bands of excitation and registration of the fluorescence are determined.

  4. Fluorescence Imaging Reveals Surface Contamination

    Science.gov (United States)

    Schirato, Richard; Polichar, Raulf

    1992-01-01

    In technique to detect surface contamination, object inspected illuminated by ultraviolet light to make contaminants fluoresce; low-light-level video camera views fluorescence. Image-processing techniques quantify distribution of contaminants. If fluorescence of material expected to contaminate surface is not intense, tagged with low concentration of dye.

  5. Who's who in fluorescence 2005

    CERN Document Server

    Geddes, Chris D

    2006-01-01

    The Journal of Fluorescence's third Who's Who directory publishes the names, contact details, specialty keywords, photographs, and a brief description of scientists employing fluorescence methodology and instrumentation in their working livesThe directory provides company contact details with a brief list of fluorescence-related products.

  6. Preparation and Characterization of Highly Fluorescent, Glutathione-coated Near Infrared Quantum Dots for in Vivo Fluorescence Imaging

    Directory of Open Access Journals (Sweden)

    Yoshichika Yoshioka

    2008-10-01

    Full Text Available Fluorescent probes that emit in the near-infrared (NIR, 700-1,300 nm region are suitable as optical contrast agents for in vivo fluorescence imaging because of low scattering and absorption of the NIR light in tissues. Recently, NIR quantum dots (QDs have become a new class of fluorescent materials that can be used for in vivo imaging. Compared with traditional organic fluorescent dyes, QDs have several unique advantages such as size- and composition-tunable emission, high brightness, narrow emission bands, large Stokes shifts, and high resistance to photobleaching. In this paper, we report a facile method for the preparation of highly fluorescent, water-soluble glutathione (GSH-coated NIR QDs for in vivo imaging. GSH-coated NIR QDs (GSH-QDs were prepared by surface modification of hydrophobic CdSeTe/CdS (core/shell QDs. The hydrophobic surface of the CdSeTe/CdS QDs was exchanged with GSH in tetrahydrofuran-water. The resulting GSH-QDs were monodisperse particles and stable in PBS (phosphate buffered saline, pH = 7.4. The GSH-QDs (800 nm emission were highly fluorescent in aqueous solutions (quantum yield = 22% in PBS buffer, and their hydrodynamic diameter was less than 10 nm, which is comparable to the size of proteins. The cellular uptake and viability for the GSH-QDs were examined using HeLa and HEK 293 cells. When the cells were incubated with aqueous solutions of the GSH-QDs (10 nM, the QDs were taken into the cells and distributed in the perinuclear region of both cells. After 12 hrs incubation of 4 nM of GSH-QDs, the viabilities of HeLa and HEK 293 cells were ca. 80 and 50%, respectively. As a biomedical utility of the GSH-QDs, in vivo NIRfluorescence imaging of a lymph node in a mouse is presented.

  7. The role of the second zero-dispersion wavelength in generation of supercontinua and bright-bright soliton-pairs across the zero-dispersion wavelength: erratum

    DEFF Research Database (Denmark)

    Frosz, Michael Henoch; Falk, Peter Andreas; Bang, Ole

    2007-01-01

    An erratum is presented explaining that the observation in the original paper (Optics Express, volume 13, issue 16, page 6181-6192, 2005), of a bright-bright soliton with one color in the anomalous dispersion region and the other color in the normal dispersion region was mistaken; both parts...

  8. Fluorescence fluctuation spectroscopy (FFS)

    CERN Document Server

    Tetin, Sergey

    2012-01-01

    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

  9. Fluorescent quantification of melanin.

    Science.gov (United States)

    Fernandes, Bruno; Matamá, Teresa; Guimarães, Diana; Gomes, Andreia; Cavaco-Paulo, Artur

    2016-11-01

    Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. Synthesis and characterization of bright green terbium coordination complex derived from 1,4-bis(carbonylmethyl)terephthalate: Structure and luminescence properties

    Science.gov (United States)

    Ma, Mengjiao; Li, Congcong; Shu, Dengkun; Wang, Chaohua; Xi, Peng

    2018-02-01

    A photoluminescent terbium (Tb) complex involving a novel benzoic-acid compound with a unique coordinated structure, namely 1,4-bis(carbonylmethyl)terephthalate (BCMT), has been designed and synthesized. The new coordinate structure and energy-transfer mechanism between the ligand and Tb(III) ions were investigated in detail. The results demonstrated that the BCMT-Tb(III) complex shows strong fluorescence intensity (4 × 106 a.u.) and long fluorescence lifetime (1.302 ms), owing to the favorable degree of energy matching between the triplet excited level of the ligand and the resonant level of Tb(III) ions. Based on the analysis of three-dimensional luminescence spectra, the as-prepared Tb(III) complex can be effectively excited in the range of 250-310 nm, and it shows high color purity, with a bright green appearance.

  11. Observations of Bright Massive Stars Using Small Size Telescopes

    Science.gov (United States)

    Beradze, Sopia; Kochiashvili, Nino

    2017-11-01

    The size of a telescope determines goals and objects of observations. During the latest decades it becomes more and more difficult to get photometric data of bright stars because most of telescopes of small sizes do not operate already. But there are rather interesting questions connected to the properties and evolution ties between different types of massive stars. Multi-wavelength photometric data are needed for solution of some of them. We are presenting our observational plans of bright Massive X-ray binaries, WR and LBV stars using a small size telescope. All these stars, which are presented in the poster are observational targets of Sopia Beradze's future PhD thesis. We already have got very interesting results on the reddening and possible future eruption of the massive hypergiant star P Cygni. Therefore, we decided to choose some additional interesting massive stars of different type for future observations. All Massive stars play an important role in the chemical evolution of galaxies because of they have very high mass loss - up to 10-4M⊙/a year. Our targets are on different evolutionary stages and three of them are the members of massive binaries. We plan to do UBVRI photometric observations of these stars using the 48 cm Cassegrain telescope of the Abastumani Astrophisical Observatory.

  12. Unveiling the Low Surface Brightness Stellar Peripheries of Galaxies

    Science.gov (United States)

    Ferguson, Annette M. N.

    2018-01-01

    The low surface brightness peripheral regions of galaxies contain a gold mine of information about how minor mergers and accretions have influenced their evolution over cosmic time. Enormous stellar envelopes and copious amounts of faint tidal debris are natural outcomes of the hierarchical assembly process and the search for and study of these features, albeit highly challenging, offers the potential for unrivalled insight into the mechanisms of galaxy growth. Over the last two decades, there has been burgeoning interest in probing galaxy outskirts using resolved stellar populations. Wide-field surveys have uncovered vast tidal debris features and new populations of very remote globular clusters, while deep Hubble Space Telescope photometry has provided exquisite star formation histories back to the earliest epochs. I will highlight some recent results from studies within and beyond the Local Group and conclude by briefly discussing the great potential of future facilities, such as JWST, Euclid, LSST and WFIRST, for major breakthroughs in low surface brightness galaxy periphery science.

  13. Bright PanSTARRS Nuclear Transients – what are they?

    Directory of Open Access Journals (Sweden)

    Smartt S.

    2012-12-01

    Full Text Available We present an initial analysis of 49 bright transients occurring in the nuclei of galaxies with no previous known Active Galactic Nucleus (AGN. They have been discovered as part of the PanSTARRs 3π survey, and followed up with the Liverpool Telescope. Based on colours, light curve shape, and a small number with optical spectra, these transients seem to fall into three groups. Red/fast transients are nuclear supernovae of various types. Some bright nuclear transients are blue and decay on a timescale of a few months; these may be candidates for tidal disruption events. However most of the events we have found are blue and are either still rising or decaying slowly, on a timescale of years; the few spectra we have show AGN at z ∼ 1. We argue that these transients are background AGN microlensed by stars in foreground galaxies by a factor 10–100. Monitoring such events gives us very promising prospects for measuring the structure of AGN and so testing current theories.

  14. IRAS surface brightness maps of reflection nebulae in the Pleiades

    Science.gov (United States)

    Castelaz, Michael W.; Werner, M. W.; Sellgren, K.

    1987-01-01

    Surface brightness maps at 12, 25, 60, and 100 microns were made of a 2.5 deg x 2.5 deg area of the reflection nebulae in the Pleiades by coadding IRAS scans of this region. Emission is seen surrounding 17 Tau, 20 Tau, 23 Tau, and 25 Tau in all four bands, coextensive with the visible reflection nebulosity, and extending as far as 30 arcminutes from the illuminating stars. The infrared energy distributions of the nebulae peak in the 100 micron band, but up to 40 percent of the total infrared power lies in the 12 and 25 micron bands. The brightness of the 12 and 25 micron emission and the absence of temperature gradients at these wavelengths are inconsistent with the predictions of equilibrium thermal emission models. The emission at these wavelengths appears to be the result of micron nonequilibrium emission from very small grains, or from molecules consisting of 10-100 carbon atoms, which have been excited by ultraviolet radiation from the illuminating stars.

  15. Bright Solitons in a PT-Symmetric Chain of Dimers

    Directory of Open Access Journals (Sweden)

    Omar B. Kirikchi

    2016-01-01

    Full Text Available We study the existence and stability of fundamental bright discrete solitons in a parity-time- (PT- symmetric coupler composed by a chain of dimers that is modelled by linearly coupled discrete nonlinear Schrödinger equations with gain and loss terms. We use a perturbation theory for small coupling between the lattices to perform the analysis, which is then confirmed by numerical calculations. Such analysis is based on the concept of the so-called anticontinuum limit approach. We consider the fundamental onsite and intersite bright solitons. Each solution has symmetric and antisymmetric configurations between the arms. The stability of the solutions is then determined by solving the corresponding eigenvalue problem. We obtain that both symmetric and antisymmetric onsite mode can be stable for small coupling, in contrast to the reported continuum limit where the antisymmetric solutions are always unstable. The instability is either due to the internal modes crossing the origin or the appearance of a quartet of complex eigenvalues. In general, the gain-loss term can be considered parasitic as it reduces the stability region of the onsite solitons. Additionally, we analyse the dynamic behaviour of the onsite and intersite solitons when unstable, where typically it is either in the form of travelling solitons or soliton blow-ups.

  16. Bright light treatment of depression for older adults [ISRCTN55452501

    Directory of Open Access Journals (Sweden)

    Knickerbocker Nancy C

    2005-11-01

    Full Text Available Abstract Background The incidence of insomnia and depression in the elder population is significant. It is hoped that use of light treatment for this group could provide safe, economic, and effective rapid recovery. Methods In this home-based trial we treated depressed elderly subjects with bright white (8,500 Lux and dim red ( Results Eighty-one volunteers, between 60 and 79 years old, completed the study. Both treatment and placebo groups experienced mood improvement. Average GDS scores improved 5 points, the Hamilton Depression Rating Scale (HDRS 17 scores (extracted from the self-rated SIGH-SAD-SR improved 6 points. There were no significant treatment effects or time-by-treatment interactions. No significant adverse reactions were observed in either treatment group. The assays of urine and saliva showed no significant differences between the treatment and placebo groups. The healthy control group was active earlier and slept earlier but received less light than the depressed group at baseline. Conclusion Antidepressant response to bright light treatment in this age group was not statistically superior to placebo. Both treatment and placebo groups experienced a clinically significant overall improvement of 16%.

  17. Suzaku observations of low surface brightness cluster Abell 1631

    Science.gov (United States)

    Babazaki, Yasunori; Mitsuishi, Ikuyuki; Ota, Naomi; Sasaki, Shin; Böhringer, Hans; Chon, Gayoung; Pratt, Gabriel W.; Matsumoto, Hironori

    2018-06-01

    We present analysis results for a nearby galaxy cluster Abell 1631 at z = 0.046 using the X-ray observatory Suzaku. This cluster is categorized as a low X-ray surface brightness cluster. To study the dynamical state of the cluster, we conduct four-pointed Suzaku observations and investigate physical properties of the Mpc-scale hot gas associated with the A 1631 cluster for the first time. Unlike relaxed clusters, the X-ray image shows no strong peak at the center and an irregular morphology. We perform spectral analysis and investigate the radial profiles of the gas temperature, density, and entropy out to approximately 1.5 Mpc in the east, north, west, and south directions by combining with the XMM-Newton data archive. The measured gas density in the central region is relatively low (a few ×10-4 cm-3) at the given temperature (˜2.9 keV) compared with X-ray-selected clusters. The entropy profile and value within the central region (r clusters. These features are also observed in another low surface brightness cluster, Abell 76. The spatial distributions of galaxies and the hot gas appear to be different. The X-ray luminosity is relatively lower than that expected from the velocity dispersion. A post-merger scenario may explain the observed results.

  18. Fluorescent nanodiamond for biomedicine

    International Nuclear Information System (INIS)

    Milos Nesladek

    2014-01-01

    NV centers in diamond have gained strong interest as a novel tool for quantum information processing, quantum computing and quantum photonics. These applications are based on fluorescent and spin properties of NV-centres. However, in some conditions NV- can lose an electron and turn to NV0. The occupation of NV0 and NV- charge states depend on the position of their ground states with respect to the Fermi level and the mechanism of the charge transfer. Interestingly, that the charge switch has important implications on applications of fluorescent nanodiamond (fND) to nano-biology and nano-medicine. fND can be used for bio-marking and bio-tracking but also for the monitoring of targeted delivery to the cells. In this presentation we review the current state-of-the art for using fND particles for fluorescent bio imaging in cells and discuss the charge transfer and its luminescence stability by using ultra high sensitive spectroscopy methods to study the NV0 and NV- state occupation. (author)

  19. BRITE-Constellation: Nanosatellites for precision photometry of bright stars

    Science.gov (United States)

    Weiss, W. W.; Moffat, A. F. J.; Schwarzenberg-Czerny, A.; Koudelka, O. F.; Grant, C. C.; Zee, R. E.; Kuschnig, R.; Mochnacki, St.; Rucinski, S. M.; Matthews, J. M.; Orleański, P.; Pamyatnykh, A. A.; Pigulski, A.; Alves, J.; Guedel, M.; Handler, G.; Wade, G. A.; Scholtz, A. L.; Scholtz

    2014-02-01

    BRITE-Constellation (where BRITE stands for BRIght Target Explorer) is an international nanosatellite mission to monitor photometrically, in two colours, brightness and temperature variations of stars brighter than V ~ 4, with precision and time coverage not possible from the ground. The current mission design consists of three pairs of 7 kg nanosats (hence ``Constellation'') from Austria, Canada and Poland carrying optical telescopes (3 cm aperture) and CCDs. One instrument in each pair is equipped with a blue filter; the other, a red filter. The first two nanosats (funded by Austria) are UniBRITE, designed and built by UTIAS-SFL (University of Toronto Institute for Aerospace Studies-Space Flight Laboratory) and its twin, BRITE-Austria, built by the Technical University Graz (TUG) with support of UTIAS-SFL. They were launched on 25 February 2013 by the Indian Space Agency, under contract to the Canadian Space Agency. Each BRITE instrument has a wide field of view (~ 24 degrees), so up to 15 bright stars can be observed simultaneously in 32 × 32 sub-rasters. Photometry (with reduced precision but thorough time sampling) of additional fainter targets will be possible through on-board data processing. A critical technical element of the BRITE mission is the three-axis attitude control system to stabilize a nanosat with very low inertia. The pointing stability is better than 1.5 arcminutes rms, a significant advance by UTIAS-SFL over any previous nanosatellite. BRITE-Constellation will primarily measure p- and g-mode pulsations to probe the interiors and ages of stars through asteroseismology. The BRITE sample of many of the brightest stars in the night sky is dominated by the most intrinsically luminous stars: massive stars seen at all evolutionary stages, and evolved medium-mass stars at the very end of their nuclear burning phases (cool giants and AGB stars). The Hertzsprung-Russell diagram for stars brighter than mag V=4 from which the BRITE-Constellation sample

  20. Novel Fluorescent Microemulsion: Probing Properties, Investigating Mechanism, and Unveiling Potential Application.

    Science.gov (United States)

    Hou, Mengna; Dang, Leping; Liu, Tiankuo; Guo, Yun; Wang, Zhanzhong

    2017-08-09

    Nanoscale microemulsions have been utilized as delivery carriers for nutraceuticals and active biological drugs. Herein, we designed and synthesized a novel oil in water (O/W) fluorescent microemulsion based on isoamyl acetate, polyoxyethylene castor oil EL (CrEL), and water. The microemulsion emitted bright blue fluorescence, thus exhibiting its potential for active drug detection with label-free strategy. The microemulsion exhibited excitation-dependent emission and distinct red shift with longer excitation wavelengths. Lifetime and quantum yield of fluorescent microemulsion were 2.831 ns and 5.0%, respectively. An excellent fluorescent stability of the microemulsion was confirmed by altering pH, ionic strength, temperature, and time. Moreover, we proposed a probable mechanism of fluorochromic phenomenon, in connection with the aromatic ring structure of polyoxyethylene ether substituent in CrEL. Based on our findings, we concluded that this new fluorescent microemulsion is a promising drug carrier that can facilitate active drug detection with a label-free strategy. Although further research is required to understand the exact mechanism behind its fluorescence property, this work provided valuable guidance to develop new biosensors based on fluorescent microemulsion.

  1. Fluorescence Stability of Mercaptopropionic Acid Capped Cadmium Telluride Quantum Dots in Various Biochemical Buffers.

    Science.gov (United States)

    Borse, Vivek; Kashikar, Adisha; Srivastava, Rohit

    2018-04-01

    Quantum dots are the semiconductor nanocrystals having unique optical and electronic properties. Quantum dots are category of fluorescent labels utilized for biological tagging, biosensing, bioassays, bioimaging and in vivo imaging as they exhibit very small size, signal brightness, photostability, tuning of light emission range, longer photoluminescence decay time as compared to organic dyes. In this work, we have synthesized and characterized mercaptopropionic acid capped cadmium telluride quantum dots (MPA-CdTe QDs) using hydrothermal method. The study further reports fluorescence intensity stability of quantum dots suspended in different buffers of varying concentration (1-100 mM), stored at various photophysical conditions. Fluorescence intensity values were reduced with increase in buffer concentration. When the samples were stored at room temperature in ambient light condition the quantum dots suspended in different buffers lost the fluorescence intensity after day 15 (except TRIS II). Fluorescence intensity values were found stable for more than 30 days when the samples were stored in dark condition. Samples stored in refrigerator displayed modest fluorescence intensity even after 300 days of storage. Thus, storage of MPA-CdTe QDs in refrigerator may be the suitable choice to maintain its fluorescence stability for longer time for further application.

  2. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.

    Science.gov (United States)

    Plamont, Marie-Aude; Billon-Denis, Emmanuelle; Maurin, Sylvie; Gauron, Carole; Pimenta, Frederico M; Specht, Christian G; Shi, Jian; Quérard, Jérôme; Pan, Buyan; Rossignol, Julien; Moncoq, Karine; Morellet, Nelly; Volovitch, Michel; Lescop, Ewen; Chen, Yong; Triller, Antoine; Vriz, Sophie; Le Saux, Thomas; Jullien, Ludovic; Gautier, Arnaud

    2016-01-19

    This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as the green fluorescent protein, enabling fluorescent labeling of proteins in a reversible and specific manner through the reversible binding and activation of a cell-permeant and nontoxic fluorogenic ligand (a so-called fluorogen). A unique fluorogen activation mechanism based on two spectroscopic changes, increase of fluorescence quantum yield and absorption red shift, provides high labeling selectivity. Y-FAST was engineered from the 14-kDa photoactive yellow protein by directed evolution using yeast display and fluorescence-activated cell sorting. Y-FAST is as bright as common fluorescent proteins, exhibits good photostability, and allows the efficient labeling of proteins in various organelles and hosts. Upon fluorogen binding, fluorescence appears instantaneously, allowing monitoring of rapid processes in near real time. Y-FAST distinguishes itself from other tagging systems because the fluorogen binding is highly dynamic and fully reversible, which enables rapid labeling and unlabeling of proteins by addition and withdrawal of the fluorogen, opening new exciting prospects for the development of multiplexing imaging protocols based on sequential labeling.

  3. Effective scheme of photolysis of GFP in live cell as revealed with confocal fluorescence microscopy

    Science.gov (United States)

    Glazachev, Yu I.; Orlova, D. Y.; Řezníčková, P.; Bártová, E.

    2018-05-01

    We proposed an effective kinetics scheme of photolysis of green fluorescent protein (GFP) observed in live cells with a commercial confocal fluorescence microscope. We investigated the photolysis of GFP-tagged heterochromatin protein, HP1β-GFP, in live nucleus with the pulse position modulation approach, which has several advantages over the classical pump-and-probe method. At the basis of the proposed scheme lies a process of photoswitching from the native fluorescence state to the intermediate fluorescence state, which has a lower fluorescence yield and recovers back to native state in the dark. This kinetics scheme includes four effective parameters (photoswitching, reverse switching, photodegradation rate constants, and relative brightness of the intermediate state) and covers the time scale from dozens of milliseconds to minutes of the experimental fluorescence kinetics. Additionally, the applicability of the scheme was demonstrated in the cases of continuous irradiation and the classical pump-and-probe approach using numerical calculations and analytical solutions. An interesting finding of experimental data analysis was that the overall photodegradation of GFP proceeds dominantly from the intermediate state, and demonstrated approximately the second-order reaction versus irradiation power. As a practical example, the proposed scheme elucidates the artifacts of fluorescence recovery after the photobleaching method, and allows us to propose some suggestions on how to diminish them.

  4. Micro Coronal Bright Points Observed in the Quiet Magnetic Network by SOHO/EIT

    Science.gov (United States)

    Falconer, D. A.; Moore, R. L.; Porter, J. G.

    1997-01-01

    When one looks at SOHO/EIT Fe XII images of quiet regions, one can see the conventional coronal bright points (> 10 arcsec in diameter), but one will also notice many smaller faint enhancements in brightness (Figure 1). Do these micro coronal bright points belong to the same family as the conventional bright points? To investigate this question we compared SOHO/EIT Fe XII images with Kitt Peak magnetograms to determine whether the micro bright points are in the magnetic network and mark magnetic bipoles within the network. To identify the coronal bright points, we applied a picture frame filter to the Fe XII images; this brings out the Fe XII network and bright points (Figure 2) and allows us to study the bright points down to the resolution limit of the SOHO/EIT instrument. This picture frame filter is a square smoothing function (hlargelyalf a network cell wide) with a central square (quarter of a network cell wide) removed so that a bright point's intensity does not effect its own background. This smoothing function is applied to the full disk image. Then we divide the original image by the smoothed image to obtain our filtered image. A bright point is defined as any contiguous set of pixels (including diagonally) which have enhancements of 30% or more above the background; a micro bright point is any bright point 16 pixels or smaller in size. We then analyzed the bright points that were fully within quiet regions (0.6 x 0.6 solar radius) centered on disk center on six different days.

  5. Bright, water-soluble CeF{sub 3} photo-, cathodo-, and X-ray luminescent nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Clement, Sandhya; Deng, Wei; Drozdowicz-Tomsia, Krystyna; Liu, Deming [Macquarie University, Centre for Nanoscale BioPhotonics, Department of Physics and Astronomy (Australia); Zachreson, Cameron [University of Technology Sydney, School of Physics and Advanced Materials (Australia); Goldys, Ewa M., E-mail: ewa.goldys@mq.edu.au [Macquarie University, Centre for Nanoscale BioPhotonics, Department of Physics and Astronomy (Australia)

    2015-01-15

    Bright, water-soluble CeF{sub 3} nanoparticles with small size and narrow size distribution have been synthesized using a simple co-precipitation method without any ligands. Size control of nanoparticles from 13 ± 2 to 9 ± 2 nm was achieved by varying the reaction time. Colloidal properties have been found to vary with pH and, independently, with dilution. The photoluminescence of the as-synthesized nanoparticles shows a highly photostable UV/Visible fluorescence band due to allowed 5d–4f transitions, also observed in the X-ray luminescence spectrum. This band is suitable for X-ray excitation of a range of photosensitizers. The photoluminescence quantum yield of nanoparticles was also determined to be 31 %. Using the measured fluorescence decay time of 25 ns, the radiative lifetime of Ce in CeF{sub 3} was found to be 80.6 ns. Both photoluminescence and cathodoluminescence emission are affected by the reaction time and measurement temperature. Electron-beam-induced defect annealing is also observed.

  6. On-chip multiplexed solid-phase nucleic acid hybridization assay using spatial profiles of immobilized quantum dots and fluorescence resonance energy transfer.

    Science.gov (United States)

    Noor, M Omair; Tavares, Anthony J; Krull, Ulrich J

    2013-07-25

    A microfluidic based solid-phase assay for the multiplexed detection of nucleic acid hybridization using quantum dot (QD) mediated fluorescence resonance energy transfer (FRET) is described herein. The glass surface of hybrid glass-polydimethylsiloxane (PDMS) microfluidic channels was chemically modified to assemble the biorecognition interface. Multiplexing was demonstrated using a detection system that was comprised of two colors of immobilized semi-conductor QDs and two different oligonucleotide probe sequences. Green-emitting and red-emitting QDs were paired with Cy3 and Alexa Fluor 647 (A647) labeled oligonucleotides, respectively. The QDs served as energy donors for the transduction of dye labeled oligonucleotide targets. The in-channel assembly of the biorecognition interface and the subsequent introduction of oligonucleotide targets was accomplished within minutes using a combination of electroosmotic flow and electrophoretic force. The concurrent quantification of femtomole quantities of two target sequences was possible by measuring the spatial coverage of FRET sensitized emission along the length of the channel. In previous reports, multiplexed QD-FRET hybridization assays that employed a ratiometric method for quantification had challenges associated with lower analytical sensitivity arising from both donor and acceptor dilution that resulted in reduced energy transfer pathways as compared to single-color hybridization assays. Herein, a spatial method for quantification that is based on in-channel QD-FRET profiles provided higher analytical sensitivity in the multiplexed assay format as compared to single-color hybridization assays. The selectivity of the multiplexed hybridization assays was demonstrated by discrimination between a fully-complementary sequence and a 3 base pair sequence at a contrast ratio of 8 to 1. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Paper-based solid-phase nucleic acid hybridization assay using immobilized quantum dots as donors in fluorescence resonance energy transfer.

    Science.gov (United States)

    Noor, M Omair; Shahmuradyan, Anna; Krull, Ulrich J

    2013-02-05

    A paper-based solid-phase assay is presented for transduction of nucleic acid hybridization using immobilized quantum dots (QDs) as donors in fluorescence resonance energy transfer (FRET). The surface of paper was modified with imidazole groups to immobilize QD-probe oligonucleotide conjugates that were assembled in solution. Green-emitting QDs (gQDs) were FRET-paired with Cy3 acceptor. Hybridization of Cy3-labeled oligonucleotide targets provided the proximity required for FRET-sensitized emission from Cy3, which served as an analytical signal. The assay exhibited rapid transduction of nucleic acid hybridization within minutes. Without any amplification steps, the limit of detection of the assay was found to be 300 fmol with the upper limit of the dynamic range at 5 pmol. The implementation of glutathione-coated QDs for the development of nucleic acid hybridization assay integrated on a paper-based platform exhibited excellent resistance to nonspecific adsorption of oligonucleotides and showed no reduction in the performance of the assay in the presence of large quantities of noncomplementary DNA. The selectivity of nucleic acid hybridization was demonstrated by single-nucleotide polymorphism (SNP) detection at a contrast ratio of 19 to 1. The reuse of paper over multiple cycles of hybridization and dehybridization was possible, with less than 20% reduction in the performance of the assay in five cycles. This work provides an important framework for the development of paper-based solid-phase QD-FRET nucleic acid hybridization assays that make use of a ratiometric approach for detection and analysis.

  8. Toward a solid-phase nucleic acid hybridization assay within microfluidic channels using immobilized quantum dots as donors in fluorescence resonance energy transfer.

    Science.gov (United States)

    Chen, Lu; Algar, W Russ; Tavares, Anthony J; Krull, Ulrich J

    2011-01-01

    The optical properties and surface area of quantum dots (QDs) have made them an attractive platform for the development of nucleic acid biosensors based on fluorescence resonance energy transfer (FRET). Solid-phase assays based on FRET using mixtures of immobilized QD-oligonucleotide conjugates (QD biosensors) have been developed. The typical challenges associated with solid-phase detection strategies include non-specific adsorption, slow kinetics of hybridization, and sample manipulation. The new work herein has considered the immobilization of QD biosensors onto the surfaces of microfluidic channels in order to address these challenges. Microfluidic flow can be used to dynamically control stringency by adjustment of the potential in an electrokinetic-based microfluidics environment. The shearing force, Joule heating, and the competition between electroosmotic and electrophoretic mobilities allow the optimization of hybridization conditions, convective delivery of target to the channel surface to speed hybridization, amelioration of adsorption, and regeneration of the sensing surface. Microfluidic flow can also be used to deliver (for immobilization) and remove QD biosensors. QDs that were conjugated with two different oligonucleotide sequences were used to demonstrate feasibility. One oligonucleotide sequence on the QD was available as a linker for immobilization via hybridization with complementary oligonucleotides located on a glass surface within a microfluidic channel. A second oligonucleotide sequence on the QD served as a probe to transduce hybridization with target nucleic acid in a sample solution. A Cy3 label on the target was excited by FRET using green-emitting CdSe/ZnS QD donors and provided an analytical signal to explore this detection strategy. The immobilized QDs could be removed under denaturing conditions by disrupting the duplex that was used as the surface linker and thus allowed a new layer of QD biosensors to be re-coated within the channel

  9. Paper-based solid-phase multiplexed nucleic acid hybridization assay with tunable dynamic range using immobilized quantum dots as donors in fluorescence resonance energy transfer.

    Science.gov (United States)

    Noor, M Omair; Krull, Ulrich J

    2013-08-06

    A multiplexed solid-phase nucleic acid hybridization assay on a paper-based platform is presented using multicolor immobilized quantum dots (QDs) as donors in fluorescence resonance energy transfer (FRET). The surface of paper was modified with imidazole groups to immobilize two types of QD-probe oligonucleotide conjugates that were assembled in solution. Green-emitting QDs (gQDs) and red-emitting QDs (rQDs) served as donors with Cy3 and Alexa Fluor 647 (A647) acceptors. The gQD/Cy3 FRET pair served as an internal standard, while the rQD/A647 FRET pair served as a detection channel, combining the control and analytical test zones in one physical location. Hybridization of dye-labeled oligonucleotide targets provided the proximity for FRET sensitized emission from the acceptor dyes, which served as an analytical signal. Hybridization assays in the multicolor format provided a limit of detection of 90 fmol and an upper limit of dynamic range of 3.5 pmol. The use of an array of detection zones was designed to provide improved analytical figures of merit compared to that which could be achieved on one type of array design in terms of relative concentration of multicolor QDs. The hybridization assays showed excellent resistance to nonspecific adsorption of oligonucleotides. Selectivity of the two-plex hybridization assay was demonstrated by single nucleotide polymorphism (SNP) detection at a contrast ratio of 50:1. Additionally, it is shown that the use of preformed QD-probe oligonucleotide conjugates and consideration of the relative number density of the two types of QD-probe conjugates in the two-color assay format is advantageous to maximize assay sensitivity and the upper limit of dynamic range.

  10. Effect of evening exposure to bright or dim light after daytime bright light on absorption of dietary carbohydrates the following morning.

    Science.gov (United States)

    Hirota, Naoko; Sone, Yoshiaki; Tokura, Hiromi

    2010-01-01

    We had previously reported on the effect of exposure to light on the human digestive system: daytime bright light exposure has a positive effect, whereas, evening bright light exposure has a negative effect on the efficiency of dietary carbohydrate absorption from the evening meal. These results prompted us to examine whether the light intensity to which subjects are exposed in the evening affects the efficiency of dietary carbohydrate absorption the following morning. In this study, subjects were exposed to either 50 lux (dim light conditions) or 2,000 lux (bright light conditions) in the evening for 9 h (from 15:00 to 24:00) after staying under bright light in the daytime (under 2,000 lux from 07:00 to 15:00). We measured unabsorbed dietary carbohydrates using the breath-hydrogen test the morning after exposure to either bright light or dim light the previous evening. Results showed that there was no significant difference between the two conditions in the amount of breath hydrogen. This indicates that evening exposure to bright or dim light after bright light exposure in the daytime has no varying effect on digestion or absorption of dietary carbohydrates in the following morning's breakfast.

  11. Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga

    Science.gov (United States)

    2015-01-01

    Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is one of the major environmental pollutants that causes mutagenesis and cancer. BaP has been shown to accumulate in phytoplankton and zooplankton. We have studied the localization and aggregation of BaP in Chlorella sp., a microalga that is one of the primary producers in the food chain, using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor approach to characterize the location and the aggregation of BaP in the cell. Our results show that BaP accumulates in the lipid bodies of Chlorella sp. and that there is Förster resonance energy transfer between BaP and photosystems of Chlorella sp., indicating the close proximity of the two molecular systems. The lifetime of BaP fluorescence was measured to be 14 ns in N,N-dimethylformamide, an average of 7 ns in Bold’s basal medium, and 8 ns in Chlorella cells. Number and brightness analysis suggests that BaP does not aggregate inside Chlorella sp. (average brightness = 5.330), while it aggregates in the supernatant. In Chlorella grown in sediments spiked with BaP, in 12 h the BaP uptake could be visualized using fluorescence microscopy. PMID:25020149

  12. Modelling of microcracks image treated with fluorescent dye

    Science.gov (United States)

    Glebov, Victor; Lashmanov, Oleg U.

    2015-06-01

    The main reasons of catastrophes and accidents are high level of wear of equipment and violation of the production technology. The methods of nondestructive testing are designed to find out defects timely and to prevent break down of aggregates. These methods allow determining compliance of object parameters with technical requirements without destroying it. This work will discuss dye penetrant inspection or liquid penetrant inspection (DPI or LPI) methods and computer model of microcracks image treated with fluorescent dye. Usually cracks on image look like broken extended lines with small width (about 1 to 10 pixels) and ragged edges. The used method of inspection allows to detect microcracks with depth about 10 or more micrometers. During the work the mathematical model of image of randomly located microcracks treated with fluorescent dye was created in MATLAB environment. Background noises and distortions introduced by the optical systems are considered in the model. The factors that have influence on the image are listed below: 1. Background noise. Background noise is caused by the bright light from external sources and it reduces contrast on the objects edges. 2. Noises on the image sensor. Digital noise manifests itself in the form of randomly located points that are differing in their brightness and color. 3. Distortions caused by aberrations of optical system. After passing through the real optical system the homocentricity of the bundle of rays is violated or homocentricity remains but rays intersect at the point that doesn't coincide with the point of the ideal image. The stronger the influence of the above-listed factors, the worse the image quality and therefore the analysis of the image for control of the item finds difficulty. The mathematical model is created using the following algorithm: at the beginning the number of cracks that will be modeled is entered from keyboard. Then the point with random position is choosing on the matrix whose size is

  13. Fluorescent microthermographic imaging

    Energy Technology Data Exchange (ETDEWEB)

    Barton, D.L.

    1993-09-01

    In the early days of microelectronics, design rules and feature sizes were large enough that sub-micron spatial resolution was not needed. Infrared or IR thermal techniques were available that calculated the object`s temperature from infrared emission. There is a fundamental spatial resolution limitation dependent on the wavelengths of light being used in the image formation process. As the integrated circuit feature sizes began to shrink toward the one micron level, the limitations imposed on IR thermal systems became more pronounced. Something else was needed to overcome this limitation. Liquid crystals have been used with great success, but they lack the temperature measurement capabilities of other techniques. The fluorescent microthermographic imaging technique (FMI) was developed to meet this need. This technique offers better than 0.01{degrees}C temperature resolution and is diffraction limited to 0.3 {mu}m spatial resolution. While the temperature resolution is comparable to that available on IR systems, the spatial resolution is much better. The FMI technique provides better spatial resolution by using a temperature dependent fluorescent film that emits light at 612 nm instead of the 1.5 {mu}m to 12 {mu}m range used by IR techniques. This tutorial starts with a review of blackbody radiation physics, the process by which all heated objects emit radiation to their surroundings, in order to understand the sources of information that are available to characterize an object`s surface temperature. The processes used in infrared thermal imaging are then detailed to point out the limitations of the technique but also to contrast it with the FMI process. The FMI technique is then described in detail, starting with the fluorescent film physics and ending with a series of examples of past applications of FMI.

  14. Large-area and bright pulsed electroluminescence in monolayer semiconductors

    KAUST Repository

    Lien, Der-Hsien; Amani, Matin; Desai, Sujay B.; Ahn, Geun Ho; Han, Kevin; He, Jr-Hau; Ager, Joel W.; Wu, Ming C.; Javey, Ali

    2018-01-01

    Transition-metal dichalcogenide monolayers have naturally terminated surfaces and can exhibit a near-unity photoluminescence quantum yield in the presence of suitable defect passivation. To date, steady-state monolayer light-emitting devices suffer from Schottky contacts or require complex heterostructures. We demonstrate a transient-mode electroluminescent device based on transition-metal dichalcogenide monolayers (MoS, WS, MoSe, and WSe) to overcome these problems. Electroluminescence from this dopant-free two-terminal device is obtained by applying an AC voltage between the gate and the semiconductor. Notably, the electroluminescence intensity is weakly dependent on the Schottky barrier height or polarity of the contact. We fabricate a monolayer seven-segment display and achieve the first transparent and bright millimeter-scale light-emitting monolayer semiconductor device.

  15. Hacking commercial quantum cryptography systems by tailored bright illumination

    Science.gov (United States)

    Lydersen, Lars; Wiechers, Carlos; Wittmann, Christoffer; Elser, Dominique; Skaar, Johannes; Makarov, Vadim

    2010-10-01

    The peculiar properties of quantum mechanics allow two remote parties to communicate a private, secret key, which is protected from eavesdropping by the laws of physics. So-called quantum key distribution (QKD) implementations always rely on detectors to measure the relevant quantum property of single photons. Here we demonstrate experimentally that the detectors in two commercially available QKD systems can be fully remote-controlled using specially tailored bright illumination. This makes it possible to tracelessly acquire the full secret key; we propose an eavesdropping apparatus built from off-the-shelf components. The loophole is likely to be present in most QKD systems using avalanche photodiodes to detect single photons. We believe that our findings are crucial for strengthening the security of practical QKD, by identifying and patching technological deficiencies.

  16. Relationships between brightness of nighttime lights and population density

    Science.gov (United States)

    Naizhuo, Z.

    2012-12-01

    Brightness of nighttime lights has been proven to be a good proxy for socioeconomic and demographic statistics. Moreover, the satellite nighttime lights data have been used to spatially disaggregate amounts of gross domestic product (GDP), fossil fuel carbon dioxide emission, and electric power consumption (Ghosh et al., 2010; Oda and Maksyutov, 2011; Zhao et al., 2012). Spatial disaggregations were performed in these previous studies based on assumed linear relationships between digital number (DN) value of pixels in the nighttime light images and socioeconomic data. However, reliability of the linear relationships was never tested due to lack of relative high-spatial-resolution (equal to or finer than 1 km × 1 km) statistical data. With the similar assumption that brightness linearly correlates to population, Bharti et al. (2011) used nighttime light data as a proxy for population density and then developed a model about seasonal fluctuations of measles in West Africa. The Oak Ridge National Laboratory used sub-national census population data and high spatial resolution remotely-sensed-images to produce LandScan population raster datasets. The LandScan population datasets have 1 km × 1 km spatial resolution which is consistent with the spatial resolution of the nighttime light images. Therefore, in this study I selected 2008 LandScan population data as baseline reference data and the contiguous United State as study area. Relationships between DN value of pixels in the 2008 Defense Meteorological Satellite Program's Operational Linescan System (DMSP-OLS) stable light image and population density were established. Results showed that an exponential function can more accurately reflect the relationship between luminosity and population density than a linear function. Additionally, a certain number of saturated pixels with DN value of 63 exist in urban core areas. If directly using the exponential function to estimate the population density for the whole brightly

  17. Design of ultrahigh brightness solar-pumped disk laser.

    Science.gov (United States)

    Liang, Dawei; Almeida, Joana

    2012-09-10

    To significantly improve the solar-pumped laser beam brightness, a multi-Fresnel lens scheme is proposed for side-pumping either a single-crystal Nd:YAG or a core-doped ceramic Sm(3+) Nd:YAG disk. Optimum laser system parameters are found through ZEMAX and LASCAD numerical analysis. An ultrahigh laser beam figure of merit B of 53 W is numerically calculated, corresponding to a significant enhancement of more than 180 times over the previous record. 17.7 W/m(2) collection efficiency is also numerically attained. The strong thermal effects that have hampered present-day rod-type solar-pumped lasers can also be largely alleviated.

  18. PG 1553 + 11 - A bright optically selected BL Lacertae object

    International Nuclear Information System (INIS)

    Falomo, R.; Treves, A.

    1990-01-01

    A detailed study of the bright optically selected BL Lac object PG 1553 + 11 is presented. UV observations, obtained during a high state of the source, together with simultaneous optical spectrophotometry and near-IR photometry, allow the spectral flux distribution to be examined from 8 x 10 to the 13th to 2.5 x 10 to the 15th Hz. This distribution is compared with that derived from quasi-simultaneous observations obtained when the source was a factor of about 3 fainter. It is found that, in the higher state, the spectrum can be described by two power laws connected by a break at about 10 to the 15th Hz, while in the low state the shape is more complex. The overall spectrum of the object is compared with the average energy distribution of X-ray and radio-selected BL Lac objects, showing that it is closer to the former class. 25 refs

  19. Beyond the Kepler/K2 bright limit

    DEFF Research Database (Denmark)

    White, T. R.; Pope, B. J. S.; Antoci, V.

    2017-01-01

    The most powerful tests of stellar models come from the brightest stars in the sky, for which complementary techniques, such as astrometry, asteroseismology, spectroscopy and interferometry, can be combined. The K2 mission is providing a unique opportunity to obtain high-precision photometric time...... have developed a new photometric technique, which we call halo photometry, to observe very bright stars using a limited number of pixels. Halo photometry is simple, fast and does not require extensive pixel allocation, and will allow us to use K2 and other photometric missions, such as TESS, to observe...... to 2000 ppm. For the star Maia, we demonstrate the utility of combining K2 photometry with spectroscopy and interferometry to show that it is not a 'Maia variable', and to establish that its variability is caused by rotational modulation of a large chemical spot on a 10 d time-scale....

  20. ROLE OF DIAMOND SECONDARY EMITTERS IN HIGH BRIGHTNESS ELECTRON SOURCES

    International Nuclear Information System (INIS)

    2005-01-01

    In this paper we explore the possibility of using diamond secondary emitter in a high average current electron injector to amplify the current from the photocathode and to isolate the cathode and the injector from each other to increase the life time of the cathode and preserve the performance of the injector. Secondary electron yield of 225 and current density of 0.8 a/cm 2 have been measured in the transmission mode from type 2 a natural diamond. Although the diamond will be heated during normal operation in the injector, calculations indicate that by cryogenically cooling the diamond, the temperature gradient along the diamond can be maintained within the acceptable range. The electron energy and temporal distributions are expected to be narrow from this device resulting in high brightness beams. Plans are underway to measure the SEY in emission mode, fabricate photocathode-diamond capsule and test diamond and capsule in superconducting RF injector

  1. A new, bright and hard aluminum surface produced by anodization

    Science.gov (United States)

    Hou, Fengyan; Hu, Bo; Tay, See Leng; Wang, Yuxin; Xiong, Chao; Gao, Wei

    2017-07-01

    Anodized aluminum (Al) and Al alloys have a wide range of applications. However, certain anodized finishings have relatively low hardness, dull appearance and/or poor corrosion resistance, which limited their applications. In this research, Al was first electropolished in a phosphoric acid-based solution, then anodized in a sulfuric acid-based solution under controlled processing parameters. The anodized specimen was then sealed by two-step sealing method. A systematic study including microstructure, surface morphology, hardness and corrosion resistance of these anodized films has been conducted. Results show that the hardness of this new anodized film was increased by a factor of 10 compared with the pure Al metal. Salt spray corrosion testing also demonstrated the greatly improved corrosion resistance. Unlike the traditional hard anodized Al which presents a dull-colored surface, this newly developed anodized Al alloy possesses a very bright and shiny surface with good hardness and corrosion resistance.

  2. High-brightness electron source driven by laser

    International Nuclear Information System (INIS)

    Zhao Kui; Geng Rongli; Wang Lifang

    1996-01-01

    A DC high-brightness laser driven by photo emissive electron gun is being developed at Beijing University, in order to produce 50∼100 ps electron bunches of high quality. The gun consists of a photocathode preparation chamber and a DC acceleration cavity. Different ways of fabricating photocathode, such as chemical vapor deposition, ion beam implantation and ion beam enhanced deposition, can be adopted. The acceleration gap is designed with the aid of simulation codes EGUN and POISSON. 100 kV DC high voltage is fed to the anode through a careful designed ceramic insulator. The laser system is a mode locked Nd-YAG oscillator proceeded by an amplifier at 10 Hz repetition rate, which can deliver three different wavelength (1064/532/266 nm). The combination of a superconducting cavity with the photocathode preparation chamber is discussed

  3. Efficiency of observer brightness discrimination in original and subracted images

    International Nuclear Information System (INIS)

    Swensson, R.G.; Kazda, I.; Nawfel, R.; Judy, P.F.

    1990-01-01

    This paper reports that, for an optimal image calculation, discriminating pairs of objects that differ only in brightness is equivalent to discriminating polarity differences in their subtraction images. This experiment measured and compared how efficiently human observers could perform the two different discriminations posed by such original and subtracted images. Disks of equal size, separated by their diameter, were superimposed on incorrelated, Gaussian noise backgrounds at different contrasts that made the two disks readily visible on the displayed radiographs. The digitally subtracted image-regions containing the two disks of each pair (shifted to registration) produced subtraction images with low-contrast disks that were either brighter or darker than the background. Observer performances in each task (measured by receiver operating characteristic [ROC] analysis) was compared with that of an optimal calculation (cross- correlator)

  4. Criteria for emittance compensation in high-brightness photoinjectors

    Directory of Open Access Journals (Sweden)

    Chun-xi Wang

    2007-10-01

    Full Text Available A critical process in high-brightness photoinjectors is emittance compensation, which brings under control the correlated transverse emittance growth due to the linear space-charge force. Although emittance compensation has been used and studied for almost two decades, the exact criteria to achieve emittance compensation is not as clear as it should be. In this paper, a perturbative analysis of slice envelopes and emittance evolution close to any reference envelope is developed, via which space-charge and chromatic effects are investigated. A new criterion for emittance compensation is found, which is complementary to the well-known matching condition for the invariant envelope and agrees very well with simulations.

  5. High Brightness Injectors Based On Photocathode DC Gun

    International Nuclear Information System (INIS)

    B. Yunn

    2001-01-01

    Sample results of new injector design method based on a photocathode dc gun are presented, based on other work analytically proving the validity of the emittance compensation scheme for the case even when beam bunching is involved. We have designed several new injectors appropriate for different bunch charge ranges accordingly. Excellent beam quality produced by these injectors clearly shows that a photocathode dc gun can compete with a rf gun on an equal footing as the source of an electron beam for the bunch charge ranging up to 2 nano Coulomb (nC). This work therefore elevates a dc gun based injector to the preferred choice for many ongoing high brightness accelerator projects considering the proven operational stability and high average power capability of the dc gun

  6. Beam position monitors for the high brightness lattice

    International Nuclear Information System (INIS)

    Ring, T.

    1985-06-01

    Engineering developments associated with the high brightness lattice and the projected change in machine operating parameters will inherently affect the diagnostics systems and devices installed at present in the storage ring. This is particularly true of the beam position monitoring (BPI) system. The new sixteen unit cell lattice with its higher betatron tune values and the limited space available in the redesigned machine straights for fitting standard BPI vessels forces a fundamental re-evaluation of the beam position monitor system. The design aims for the new system are based on accepting the space limitations imposed while still providing the monitor points required to give good radial and vertical closed orbit plots. The locations of BPI's in the redesigned machine straights is illustrated. A description of the new BPI assemblies and their calibration is given. The BPI's use capacitance button type pick-ups; their response is described. (U.K.)

  7. Multiwavelength Coverage of a Bright Flare from Sgr A

    International Nuclear Information System (INIS)

    Trap, G.; Goldwurm, A.; Terrier, R.

    2009-01-01

    The dynamical center of our galaxy hosts a supermassive black hole, Sgr A*, which has been the target of an extensive multiwavelength campaign for a week in April 2007. We report here the detection of a bright flare from the vicinity of the horizon, observed simultaneously in X-rays (XMM-Newton) and NIR (VLT/NACO) on April 4 th . For the first time, such an event also benefitted from a soft γ-rays (INTEGRAL/ISGRI) and MIR (VLT/VISIR) coverage, which enabled us to derive upper limits at both ends of Sgr A* spectral energy distribution (SED). We discuss the physical implications of the contemporaneous light curves as well as the SED, in terms of synchrotron, synchrotron self-Compton and external Compton emission processes.

  8. Discovery of a bright quasar without a massive host galaxy.

    Science.gov (United States)

    Magain, Pierre; Letawe, Géraldine; Courbin, Frédéric; Jablonka, Pascale; Jahnke, Knud; Meylan, Georges; Wisotzki, Lutz

    2005-09-15

    A quasar is thought to be powered by the infall of matter onto a supermassive black hole at the centre of a massive galaxy. Because the optical luminosity of quasars exceeds that of their host galaxy, disentangling the two components can be difficult. This led in the 1990s to the controversial claim of the discovery of 'naked' quasars. Since then, the connection between quasars and galaxies has been well established. Here we report the discovery of a quasar lying at the edge of a gas cloud, whose size is comparable to that of a small galaxy, but whose spectrum shows no evidence for stars. The gas in the cloud is excited by the quasar itself. If a host galaxy is present, it is at least six times fainter than would normally be expected for such a bright quasar. The quasar is interacting dynamically with a neighbouring galaxy, whose gas might be feeding the black hole.

  9. Investigation of fundamental limits to beam brightness available from photoinjectors

    International Nuclear Information System (INIS)

    Bazarov, Ivan

    2015-01-01

    The goal of this project was investigation of fundamental limits to beam brightness available from photoinjectors. This basic research in accelerator physics spanned over 5 years aiming to extend the fundamental understanding of high average current, low emittance sources of relativistic electrons based on photoemission guns, a necessary prerequisite for a new generation of coherent X-ray synchrotron radiation facilities based on continuous duty superconducting linacs. The program focused on two areas critical to making advances in the electron source performance: 1) the physics of photocathodes for the production of low emittance electrons and 2) control of space charge forces in the immediate vicinity to the cathode via 3D laser pulse shaping.

  10. Large-area and bright pulsed electroluminescence in monolayer semiconductors

    KAUST Repository

    Lien, Der-Hsien

    2018-04-04

    Transition-metal dichalcogenide monolayers have naturally terminated surfaces and can exhibit a near-unity photoluminescence quantum yield in the presence of suitable defect passivation. To date, steady-state monolayer light-emitting devices suffer from Schottky contacts or require complex heterostructures. We demonstrate a transient-mode electroluminescent device based on transition-metal dichalcogenide monolayers (MoS, WS, MoSe, and WSe) to overcome these problems. Electroluminescence from this dopant-free two-terminal device is obtained by applying an AC voltage between the gate and the semiconductor. Notably, the electroluminescence intensity is weakly dependent on the Schottky barrier height or polarity of the contact. We fabricate a monolayer seven-segment display and achieve the first transparent and bright millimeter-scale light-emitting monolayer semiconductor device.

  11. Investigation of fundamental limits to beam brightness available from photoinjectors

    Energy Technology Data Exchange (ETDEWEB)

    Bazarov, Ivan [Cornell Univ., Ithaca, NY (United States)

    2015-07-09

    The goal of this project was investigation of fundamental limits to beam brightness available from photoinjectors. This basic research in accelerator physics spanned over 5 years aiming to extend the fundamental understanding of high average current, low emittance sources of relativistic electrons based on photoemission guns, a necessary prerequisite for a new generation of coherent X-ray synchrotron radiation facilities based on continuous duty superconducting linacs. The program focused on two areas critical to making advances in the electron source performance: 1) the physics of photocathodes for the production of low emittance electrons and 2) control of space charge forces in the immediate vicinity to the cathode via 3D laser pulse shaping.

  12. Three Millisecond Pulsars in Fermi LAT Unassociated Bright Sources

    Science.gov (United States)

    Ransom, S. M.; Ray, P. S.; Camilo, F.; Roberts, M. S. E.; Celik, O.; Wolff, M. T.; Cheung, C. C.; Kerr, M.; Pennucci, T.; DeCesar, M. E.; hide

    2010-01-01

    We searched for radio pulsars in 25 of the non-variable, unassociated sources in the Fermi LAT Bright Source List with the Green Bank Telescope at 820 MHz. We report the discovery of three radio and gamma-ray millisecond pulsar (MSPs) from a high Galactic latitude subset of these sources. All of the pulsars are in binary systems, which would have made them virtually impossible to detect in blind gamma-ray pulsation searches. They seem to be relatively normal, nearby (pulsars are power law in nature with exponential cutoffs at a few Ge V, as has been found with most other pulsars. The MSPs have all been detected as X-ray point sources. Their soft X-ray luminosities of approx 10(exp 30) - 10(exp 31) erg/s are typical of the rare radio MSPs seen in X-rays.

  13. IRAS bright galaxy sample. II. The sample and luminosity function

    International Nuclear Information System (INIS)

    Soifer, B.T.; Sanders, D.B.; Neugebauer, G.; Madore, B.F.; Danielson, G.E.; David Dunlap Observatory, Richmond Hill, Canada; Palomar Observatory; California Institute of Technology, Pasadena)

    1987-01-01

    A statistically complete sample of 324 of the brightest infrared galaxies discovered at 60 microns in the IRAS all-sky survey is described. The results show that far-infrared emission is a significant luminosity component in the local universe, representing 25 percent of the luminosity emitted by stars in the same volume. Above 10 to the 11th solar luminosities, the infrared luminous galaxies are the dominant population of objects in the universe, being as numerous as the Seyfert galaxies and more numerous than quasars at higher luminosities. The infrared luminosity appears to be independent of the optical luminosity of galaxies. Most infrared bright galaxies appear to require much of the interstellar matter to be contributing to the observed infrared luminosity. Approximately 60-80 percent of the far-infrared luminosity of the local universe can be attributed, directly or indirectly, to recent or ongoing star formation. 67 references

  14. Multiwavelength Study of Gamma-Ray Bright Blazars

    Science.gov (United States)

    Morozova, Daria; Larionov, V. M.; Hagen-Thorn, V. A.; Jorstad, S. G.; Marscher, A. P.; Troitskii, I. S.

    2011-01-01

    We investigate total intensity radio images of 6 gamma-ray bright blazars (BL Lac, 3C 279, 3C 273, W Com, PKS 1510-089, and 3C 66A) and their optical and gamma-ray light curves to study connections between gamma-ray and optical brightness variations and changes in the parsec-scale radio structure. We use high-resolution maps obtained by the BU group at 43 GHz with the VLBA, optical light curves constructed by the St.Petersburg State U. (Russia) team using measurements with the 0.4 m telescope of St.Petersburg State U. (LX200) and the 0.7 m telescope of the Crimean Astrophysical Observatory (AZT-8), and gamma-ray light curves, which we have constructed with data provided by the Fermi Large Area Telescope. Over the period from August 2008 to November 2009, superluminal motion is found in all 6 objects with apparent speed ranging from 2c to 40c. The blazars with faster apparent speeds, 3C 273, 3C 279, PKS 1510-089, and 3C 66A, exhibit stronger variability of the gamma-ray emission. There is a tendency for sources with sharply peaked gamma-ray flares to have faster jet speed than sources with gamma-ray light curves with no sharp peaks. Gamma-ray light curves with sharply peaked gamma-ray flares possess a stronger gamma-ray/optical correlations. The research at St.Petersburg State U. was funded by the Minister of Education and Science of the Russian Federation (state contract N#P123). The research at BU was funded in part by NASA Fermi Guest Investigator grant NNX08AV65G and by NSF grant AST-0907893. The VLBA is an instrument of the National Radio Astronomy Observatory, a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc.

  15. Suzaku observations of low surface brightness cluster Abell 1631

    Science.gov (United States)

    Babazaki, Yasunori; Mitsuishi, Ikuyuki; Ota, Naomi; Sasaki, Shin; Böhringer, Hans; Chon, Gayoung; Pratt, Gabriel W.; Matsumoto, Hironori

    2018-04-01

    We present analysis results for a nearby galaxy cluster Abell 1631 at z = 0.046 using the X-ray observatory Suzaku. This cluster is categorized as a low X-ray surface brightness cluster. To study the dynamical state of the cluster, we conduct four-pointed Suzaku observations and investigate physical properties of the Mpc-scale hot gas associated with the A 1631 cluster for the first time. Unlike relaxed clusters, the X-ray image shows no strong peak at the center and an irregular morphology. We perform spectral analysis and investigate the radial profiles of the gas temperature, density, and entropy out to approximately 1.5 Mpc in the east, north, west, and south directions by combining with the XMM-Newton data archive. The measured gas density in the central region is relatively low (a few ×10-4 cm-3) at the given temperature (˜2.9 keV) compared with X-ray-selected clusters. The entropy profile and value within the central region (r < 0.1 r200) are found to be flatter and higher (≳400 keV cm2). The observed bolometric luminosity is approximately three times lower than that expected from the luminosity-temperature relation in previous studies of relaxed clusters. These features are also observed in another low surface brightness cluster, Abell 76. The spatial distributions of galaxies and the hot gas appear to be different. The X-ray luminosity is relatively lower than that expected from the velocity dispersion. A post-merger scenario may explain the observed results.

  16. Human responses to bright light of different durations.

    Science.gov (United States)

    Chang, Anne-Marie; Santhi, Nayantara; St Hilaire, Melissa; Gronfier, Claude; Bradstreet, Dayna S; Duffy, Jeanne F; Lockley, Steven W; Kronauer, Richard E; Czeisler, Charles A

    2012-07-01

    Light exposure in the early night induces phase delays of the circadian rhythm in melatonin in humans. Previous studies have investigated the effect of timing, intensity, wavelength, history and pattern of light stimuli on the human circadian timing system. We present results from a study of the duration–response relationship to phase-delaying bright light. Thirty-nine young healthy participants (16 female; 22.18±3.62 years) completed a 9-day inpatient study. Following three baseline days, participants underwent an initial circadian phase assessment procedure in dim light (light pulse (∼10,000 lux) of 0.2 h, 1.0 h, 2.5 h or 4.0 h duration during a 4.5 h controlled-posture episode centred in a 16 h wake episode. After another 8 h sleep episode, participants completed a second circadian phase assessment. Phase shifts were calculated from the difference in the clock time of the dim light melatonin onset (DLMO) between the initial and final phase assessments. Exposure to varying durations of bright light reset the circadian pacemaker in a dose-dependent, non-linear manner. Per minute of exposure, the 0.2 h duration was over 5 times more effective at phase delaying the circadian pacemaker (1.07±0.36 h) as compared with the 4.0 h duration (2.65±0.24 h). Acute melatonin suppression and subjective sleepiness also had a dose-dependent response to light exposure duration. These results provide strong evidence for a non-linear resetting response of the human circadian pacemaker to light duration.

  17. State-Of High Brightness RF Photo-Injector Design

    Science.gov (United States)

    Ferrario, Massimo; Clendenin, Jym; Palmer, Dennis; Rosenzweig, James; Serafini, Luca

    2000-04-01

    The art of designing optimized high brightness electron RF Photo-Injectors has moved in the last decade from a cut and try procedure, guided by experimental experience and time consuming particle tracking simulations, up to a fast parameter space scanning, guided by recent analytical results and a fast running semi-analytical code, so to reach the optimum operating point which corresponds to maximum beam brightness. Scaling laws and the theory of invariant envelope provide to the designers excellent tools for a first parameters choice and the code HOMDYN, based on a multi-slice envelope description of the beam dynamics, is tailored to describe the space charge dominated dynamics of laminar beams in presence of time dependent space charge forces, giving rise to a very fast modeling capability for photo-injectors design. We report in this talk the results of a recent beam dynamics study, motivated by the need to redesign the LCLS photoinjector. During this work a new effective working point for a split RF photoinjector has been discovered by means of the previous mentioned approach. By a proper choice of rf gun and solenoid parameters, the emittance evolution shows a double minimum behavior in the drifting region. If the booster is located where the relative emittance maximum and the envelope waist occur, the second emittance minimum can be shifted at the booster exit and frozen at a very low level (0.3 mm-mrad for a 1 nC flat top bunch), to the extent that the invariant envelope matching conditions are satisfied.

  18. Advanced prior modeling for 3D bright field electron tomography

    Science.gov (United States)

    Sreehari, Suhas; Venkatakrishnan, S. V.; Drummy, Lawrence F.; Simmons, Jeffrey P.; Bouman, Charles A.

    2015-03-01

    Many important imaging problems in material science involve reconstruction of images containing repetitive non-local structures. Model-based iterative reconstruction (MBIR) could in principle exploit such redundancies through the selection of a log prior probability term. However, in practice, determining such a log prior term that accounts for the similarity between distant structures in the image is quite challenging. Much progress has been made in the development of denoising algorithms like non-local means and BM3D, and these are known to successfully capture non-local redundancies in images. But the fact that these denoising operations are not explicitly formulated as cost functions makes it unclear as to how to incorporate them in the MBIR framework. In this paper, we formulate a solution to bright field electron tomography by augmenting the existing bright field MBIR method to incorporate any non-local denoising operator as a prior model. We accomplish this using a framework we call plug-and-play priors that decouples the log likelihood and the log prior probability terms in the MBIR cost function. We specifically use 3D non-local means (NLM) as the prior model in the plug-and-play framework, and showcase high quality tomographic reconstructions of a simulated aluminum spheres dataset, and two real datasets of aluminum spheres and ferritin structures. We observe that streak and smear artifacts are visibly suppressed, and that edges are preserved. Also, we report lower RMSE values compared to the conventional MBIR reconstruction using qGGMRF as the prior model.

  19. Characterizing bars in low surface brightness disc galaxies

    Science.gov (United States)

    Peters, Wesley; Kuzio de Naray, Rachel

    2018-05-01

    In this paper, we use B-band, I-band, and 3.6 μm azimuthal light profiles of four low surface brightness galaxies (LSBs; UGC 628, F568-1, F568-3, F563-V2) to characterize three bar parameters: length, strength, and corotation radius. We employ three techniques to measure the radius of the bars, including a new method using the azimuthal light profiles. We find comparable bar radii between the I-band and 3.6 μm for all four galaxies when using our azimuthal light profile method, and that our bar lengths are comparable to those in high surface brightness galaxies (HSBs). In addition, we find the bar strengths for our galaxies to be smaller than those for HSBs. Finally, we use Fourier transforms of the B-band, I-band, and 3.6 μm images to characterize the bars as either `fast' or `slow' by measuring the corotation radius via phase profiles. When using the B- and I-band phase crossings, we find three of our galaxies have faster than expected relative bar pattern speeds for galaxies expected to be embedded in centrally dense cold dark matter haloes. When using the B-band and 3.6 μm phase crossings, we find more ambiguous results, although the relative bar pattern speeds are still faster than expected. Since we find a very slow bar in F563-V2, we are confident that we are able to differentiate between fast and slow bars. Finally, we find no relation between bar strength and relative bar pattern speed when comparing our LSBs to HSBs.

  20. Differential Rotation via Tracking of Coronal Bright Points.

    Science.gov (United States)

    McAteer, James; Boucheron, Laura E.; Osorno, Marcy

    2016-05-01

    The accurate computation of solar differential rotation is important both as a constraint for, and evidence towards, support of models of the solar dynamo. As such, the use of Xray and Extreme Ultraviolet bright points to elucidate differential rotation has been studied in recent years. In this work, we propose the automated detection and tracking of coronal bright points (CBPs) in a large set of SDO data for re-evaluation of solar differential rotation and comparison to other results. The big data aspects, and high cadence, of SDO data mitigate a few issues common to detection and tracking of objects in image sequences and allow us to focus on the use of CBPs to determine differential rotation. The high cadence of the data allows to disambiguate individual CBPs between subsequent images by allowing for significant spatial overlap, i.e., by the fact that the CBPs will rotate a short distance relative to their size. The significant spatial overlap minimizes the effects of incorrectly detected CBPs by reducing the occurrence of outlier values of differential rotation. The big data aspects of the data allows to be more conservative in our detection of CBPs (i.e., to err on the side of missing CBPs rather than detecting extraneous CBPs) while still maintaining statistically larger populations over which to study characteristics. The ability to compute solar differential rotation through the automated detection and tracking of a large population of CBPs will allow for further analyses such as the N-S asymmetry of differential rotation, variation of differential rotation over the solar cycle, and a detailed study of the magnetic flux underlying the CBPs.

  1. SURFACE BRIGHTNESS PROFILES OF DWARF GALAXIES. I. PROFILES AND STATISTICS

    International Nuclear Information System (INIS)

    Herrmann, Kimberly A.; Hunter, Deidre A.; Elmegreen, Bruce G.

    2013-01-01

    Radial surface brightness profiles of spiral galaxies are classified into three types: (I) single exponential, or the light falls off with one exponential to a break before falling off (II) more steeply, or (III) less steeply. Profile breaks are also found in dwarf disks, but some dwarf Type IIs are flat or increasing out to a break before falling off. Here we re-examine the stellar disk profiles of 141 dwarfs: 96 dwarf irregulars (dIms), 26 Blue Compact Dwarfs (BCDs), and 19 Magellanic-type spirals (Sms). We fit single, double, or even triple exponential profiles in up to 11 passbands: GALEX FUV and NUV, ground-based UBVJHK and Hα, and Spitzer 3.6 and 4.5 μm. We find that more luminous galaxies have brighter centers, larger inner and outer scale lengths, and breaks at larger radii; dwarf trends with M B extend to spirals. However, the V-band break surface brightness is independent of break type, M B , and Hubble type. Dwarf Type II and III profiles fall off similarly beyond the breaks but have different interiors and IIs break ∼twice as far as IIIs. Outer Type II and III scale lengths may have weak trends with wavelength, but pure Type II inner scale lengths clearly decrease from the FUV to visible bands whereas Type III inner scale lengths increase with redder bands. This suggests the influence of different star formation histories on profile type, but nonetheless the break location is approximately the same in all passbands. Dwarfs continue trends between profile and Hubble types such that later-type galaxies have more Type II but fewer Type I and III profiles than early-type spirals. BCDs and Sms are over-represented as Types III and II, respectively, compared to dIms

  2. A fluorescence scanning electron microscope

    International Nuclear Information System (INIS)

    Kanemaru, Takaaki; Hirata, Kazuho; Takasu, Shin-ichi; Isobe, Shin-ichiro; Mizuki, Keiji; Mataka, Shuntaro; Nakamura, Kei-ichiro

    2009-01-01

    Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.

  3. Fluorescent sensing with Fresnel microlenses for optofluidic systems

    Science.gov (United States)

    Siudzińska, Anna; Miszczuk, Andrzej; Marczak, Jacek; Komorowska, Katarzyna

    2017-05-01

    The concept of fluorescent sensing in a microchannel equipped with focusing light Fresnel lenses has been demonstrated. The concept employs a line or array of Fresnel lenses generating a line or array of focused light spots within a microfluidic channel, to increase the sensitivity of fluorescent signal detection in the system. We have presented efficient methods of master mold fabrication based on the lithography method and focused ion beam milling. The flexible microchannel was fabricated by an imprint process with new thiolene-epoxy resin with a good ability to replicate even submicron-size features. For final imprinted lenses, the measured background to peak signal level shows more than nine times the increase in brightness at the center of the focal spot for the green part of the spectrum (532 nm). The effectiveness of the microlenses in fluorescent-marked Escherichia coli bacteria was confirmed in a basic fluoroscope experiment, showing the increase of the sensitivity of the detection by the order of magnitude.

  4. Compact fluorescent lamp phosphors in accidental radiation monitoring

    International Nuclear Information System (INIS)

    Murthy, K. V. R.; Pallavi, S. P.; Ghildiyal, R.; Parmar, M. C.; Patel, Y. S.; Ravi Kumar, V.; Sai Prasad, A. S.; Natarajan, V.; Page, A. G.

    2006-01-01

    The application of lamp phosphors for accidental dosimetry is a new concept. Since the materials used in fluorescent lamps are good photo luminescent materials, if one can either use the inherent defects present in the phosphor or add suitable modifiers to induce thermoluminescence (TL) in these phosphors, then the device (fluorescent lamp) can be used as an accidental dosemeter. In continuation of our search for a suitable phosphor material, which can serve both as an efficient lamp phosphor and as a good radiation monitoring device, detailed examination has been carried out on cerium and terbium-doped lanthanum phosphate material. A 90 Sr beta source with 50 mCi strength (1.85 GBq) was used as the irradiation source for TL studies. The TL response as a function of dose received was examined for all phosphors used and it was observed that the intensity of the TL peak vs. dose received was a linear function in the dose range 0.1-200 Gy in each case. Incidentally LaPO 4 :Ce,Tb is a component of the compact fluorescent lamp marketed recently as an energy bright light source. Besides having very good luminescence efficiency, good dosimetric properties of these phosphors render them useful for their use in accidental dosimetry also. (authors)

  5. Bright solitons in coupled defocusing NLS equation supported by coupling: Application to Bose-Einstein condensation

    International Nuclear Information System (INIS)

    Adhikari, Sadhan K.

    2005-01-01

    We demonstrate the formation of bright solitons in coupled self-defocusing nonlinear Schroedinger (NLS) equation supported by attractive coupling. As an application we use a time-dependent dynamical mean-field model to study the formation of stable bright solitons in two-component repulsive Bose-Einstein condensates (BECs) supported by interspecies attraction in a quasi one-dimensional geometry. When all interactions are repulsive, there cannot be bright solitons. However, bright solitons can be formed in two-component repulsive BECs for a sufficiently attractive interspecies interaction, which induces an attractive effective interaction among bosons of same type

  6. Dark and bright solitons in a quasi-one-dimensional Bose-Einstein condensate

    International Nuclear Information System (INIS)

    Wang, Shun-Jin; Jia, Cheng-Long; An, Jun-Hong; Zhao, Dun; Luo, Hong-Gang

    2003-01-01

    The analytical dark and bright soliton solutions of the one-dimensional Gross-Pitaevskii equation with a confining potential are obtained. For the bright soliton, the recent experimental finding is studied, and the particle number of the soliton and the window of the particle numbers for the bright soliton to occur are estimated analytically and in good agreement with the experimental data. The existence of dark soliton for the attractive interaction and bright soliton for the repulsive interaction is predicted under proper conditions

  7. Bright galaxies in the Fornax cluster. Automated galaxy surface photometry: Pt. 7

    International Nuclear Information System (INIS)

    Disney, M.J.; Phillipps, S.; Davies, J.L.; Cawson, M.G.M.; Kibblewhite, E.J.

    1990-01-01

    We have determined surface-brightness profiles for all galaxies down to magnitude B = 16 in the central region of the Fornax cluster. Using existing redshift data, we have determined the distributions of surface brightness for both the whole sample and for cluster disc galaxies only. Although both distributions peak at extrapolated central surface brightness ∼ 21.7B mag/arcsec 2 (the canonical result), it is shown that they are, in fact, consistent with very broad distributions of disc central surface brightness once selection effects and the effects of bulge contamination of the profile are taken into account. (author)

  8. Observation of near-infrared surface brightness of the large Magellanic cloud

    International Nuclear Information System (INIS)

    Hayakawa, Satio; Koizumi, Yutaka; Matsumoto, Toshio; Murakami, Hiroshi; Uyama, Kiichiro.

    1981-01-01

    The near-infrared surface brightness of the large Magellanic cloud was observed by an infrared telescope carried by a balloon. The balloon flight was made at Australian Balloon Launching Station. The brightness distribution of 2.4 Mu m radiation was obtained. A part of Bar was bright, and the expansion of the contour at the east end of Bar corresponded to the 30 Dor region. Many near-infrared sources distribute in this region. Discussions on the color and brightness of the center of Bar and the 30 Dor region are presented. (Kato, T.)

  9. Photosynthetically supplemental lighting for vegetable crop production with super-bright laser diode

    Science.gov (United States)

    Hu, Yongguang; Li, Pingping; Shi, Jintong

    2007-02-01

    Although many artificial light sources like high-pressure sodium lamp, metal halide lamp, fluorescent lamp and so on are commonly used in horticulture, they are not widely applied because of the disadvantages of unreasonable spectra, high cost and complex control. Recently new light sources of light-emitting diode (LED) and laser diode (LD) are becoming more and more popular in the field of display and illumination with the improvement of material and manufacturing, long life-span and increasingly low cost. A new type of super-bright red LD (BL650, central wavelength is 650 nm) was selected to make up of the supplemental lighting panel, on which LDs were distributed with regular hexagon array. Drive circuit was designed to power it and adjust light intensity. System performance including temperature rise and light intensity distribution under different vertical/horizontal distances were tested. Photosynthesis of sweet pepper and eggplant leaf under LD was measured with LI-6400 to show the supplemental lighting effects. The results show that LD system can supply the maximum light intensity of 180 μmol/m2 •s at the distance of 50 mm below the panel and the temperature rise is little within 1 °C. Net photosynthetic rate became faster when LD system increased light intensity. Compared with sunlight and LED supplemental lighting system, LD's promotion on photosynthesis is in the middle. Thus it is feasible for LD light source to supplement light for vegetable crops. Further study would focus on the integration of LD and other artificial light sources.

  10. Development of a fluorescent cryocooler

    International Nuclear Information System (INIS)

    Edwards, B.C.; Buchwald, M.I.; Epstein, R.I.; Gosnell, T.R.; Mungan, C.E.

    1995-01-01

    Recent work at Los Alamos National Laboratory has demonstrated the physical principles for a new type of solid-state cryocooler based on anti-Stokes fluorescence. Design studies indicate that a vibration-free, low-mass ''fluorescent cryocooler'' could operate for years with efficiencies and cooling powers comparable to current commercial systems. This paper presents concepts for a fluorescent cryocooler, design considerations and expected performance

  11. Lipid nanoparticle vectorization of indocyanine green improves fluorescence imaging for tumor diagnosis and lymph node resection.

    Science.gov (United States)

    Navarro, Fabrice P; Berger, Michel; Guillermet, Stéphanie; Josserand, Véronique; Guyon, Laurent; Neumann, Emmanuelle; Vinet, Françoise; Texier, Isabelle

    2012-10-01

    Fluorescence imaging is opening a new era in image-guided surgery and other medical applications. The only FDA approved contrast agent in the near infrared is IndoCyanine Green (ICG), which despites its low toxicity, displays poor chemical and optical properties for long-term and sensitive imaging applications in human. Lipid nanoparticles are investigated for improving ICG optical properties and in vivo fluorescence imaging sensitivity. 30 nm diameter lipid nanoparticles (LNP) are loaded with ICG. Their characterization and use for tumor and lymph node imaging are described. Nano-formulation benefits dye optical properties (6 times improved brightness) and chemical stability (>6 months at 4 degrees C in aqueous buffer). More importantly, LNP vectorization allows never reported sensitive and prolonged (>1 day) labeling of tumors and lymph nodes. Composed of human-use approved ingredients, this novel ICG nanometric formulation is foreseen to expand rapidly the field of clinical fluorescence imaging applications.

  12. A fluorescent screen + CCD system for quality assurance of therapeutic scanned ion beams

    Energy Technology Data Exchange (ETDEWEB)

    Takeshita, E., E-mail: eriuli@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Furukawa, T., E-mail: t_furu@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Inaniwa, T., E-mail: taku@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Sato, S., E-mail: shin_s@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Himukai, T., E-mail: himukai@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Shirai, T., E-mail: t_shirai@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan); Noda, K., E-mail: noda_k@nirs.go.jp [National Institute of Radiological Sciences, Chiba (Japan)

    2011-12-15

    A fluorescent screen + a charge coupled device (CCD) system were developed to verify the performance of scanned ion beams at the HIMAC. The fluorescent light from the screen is observed by the CCD camera. Two-dimensional fields, produced by the scanning process, i.e., the position and the size of the beam for each scan, represent of the important issues in scanning irradiation. In the developed system, the two-dimensional relative fluence and the flatness of the irradiation field were measured in a straightforward technique from the luminance distribution on the screen. The position and the size of the beams were obtained from centroid computation results of the brightness. By the good sensitivity and spatial resolution of the fluorescent screen + CCD system, the scanned ion beams were verified as the measurements at the HIMAC prototype scanning system.

  13. A fluorescent screen + CCD system for quality assurance of therapeutic scanned ion beams

    Science.gov (United States)

    Takeshita, E.; Furukawa, T.; Inaniwa, T.; Sato, S.; Himukai, T.; Shirai, T.; Noda, K.

    2011-12-01

    A fluorescent screen + a charge coupled device (CCD) system were developed to verify the performance of scanned ion beams at the HIMAC. The fluorescent light from the screen is observed by the CCD camera. Two-dimensional fields, produced by the scanning process, i.e., the position and the size of the beam for each scan, represent of the important issues in scanning irradiation. In the developed system, the two-dimensional relative fluence and the flatness of the irradiation field were measured in a straightforward technique from the luminance distribution on the screen. The position and the size of the beams were obtained from centroid computation results of the brightness. By the good sensitivity and spatial resolution of the fluorescent screen + CCD system, the scanned ion beams were verified as the measurements at the HIMAC prototype scanning system.

  14. Improved efficiency of organic light-emitting diodes based on a europium complex by fluorescent dye

    Energy Technology Data Exchange (ETDEWEB)

    You Han [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022 (China); Fang Junfeng [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022 (China); Gao Jia [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022 (China); Ma Dongge [State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022 (China)]. E-mail: mdg1014@ciac.jl.cn

    2007-01-15

    Improved efficiency of organic light-emitting diodes (OLEDs) based on europium complexes have been realized by using a fluorescent dye 4-(dicyanomethylene)-2-t-butyl-6 (1,1,7,7-tetramethyljulolidyl-9-enyl))-4H-pyran (DCJTB) doping .The luminous efficiency of the devices with a fluorescent dye in the emissive layer was found to improve two times of that in devices without fluorescent dye. The devices showed pure red light, which is the characteristic emission of trivalent europium ion with a full-width at half-maximum of 3 nm.The maximum brightness and luminous efficiency reached 1200 cd/m{sup 2} at 23 V and 7.3 cd/A (2.0 lm/w), respectively, at a current density of 0.35 mA/cm{sup 2}.

  15. Sizes of water-soluble luminescent quantum dots measured by fluorescence correlation spectroscopy

    International Nuclear Information System (INIS)

    Zhang Pudun; Li Liang; Dong Chaoqing; Qian Huifeng; Ren Jicun

    2005-01-01

    In this paper, fluorescence correlation spectroscopy (FCS) was applied to measure the size of water-soluble quantum dots (QDs). The measurements were performed on a home-built FCS system based on the Stokes-Einstein equation. The obtained results showed that for bare CdTe QDs the sizes from FCS were larger than the ones from transmission electron microscopy (TEM). The brightness of QDs was also evaluated using FCS technique. It was found that the stability of the surface chemistry of QDs would be significantly improved by capping it with hard-core shell. Our data demonstrated that FCS is a simple, fast, and effective method for characterizing the fluorescent quantum dots, and is especially suitable for determining the fluorescent nanoparticles less than 10 nm in water solution

  16. Fluorescence of ceramic color standards

    International Nuclear Information System (INIS)

    Koo, Annette; Clare, John F.; Nield, Kathryn M.; Deadman, Andrew; Usadi, Eric

    2010-01-01

    Fluorescence has been found in color standards available for use in calibration and verification of color measuring instruments. The fluorescence is excited at wavelengths below about 600 nm and emitted above 700 nm, within the response range of silicon photodiodes, but at the edge of the response of most photomultipliers and outside the range commonly scanned in commercial colorimeters. The degree of fluorescence on two of a set of 12 glossy ceramic tiles is enough to introduce significant error when those tiles have been calibrated in one mode of measurement and are used in another. We report the nature of the fluorescence and the implications for color measurement.

  17. Mineralogical characterization and beneficiation study of kaolin from Equador (RN and Junco do Seridó (PB to increase the brightness index

    Directory of Open Access Journals (Sweden)

    V. M. J. S. Campos

    Full Text Available Abstract Kaolin is a rock composed mainly of kaolinite. It is used in many industrial segments, such as paper and ceramics. However, for these uses it is necessary to submit the ore to appropriate beneficiation, which generally involves magnetic separation and chemical bleaching, aiming to remove iron oxide and hydroxide, raising brightness index. This work reports the mineralogical characterization and analysis of the beneficiation of three samples of kaolin, two from Equador (Rio Grande do Norte - RN and the third from Junco do Seridó (Paraíba - PB. The samples were submitted to granulometric classification in sieve of 44 μm, magnetic separation in a magnetic induction of 14000 gauss, and chemical bleaching with sodium dithionite during 240 min. The processes were divided into two beneficiation routes. X-ray diffraction, chemical analysis by X-ray fluorescence and scanning electron microscopy were used for mineralogical characterization of the ore and measurement of the brightness index, while electron paramagnetic resonance was applied to study the variation of iron oxides and hydroxides during beneficiation. The results indicate that just 30 min of chemical bleaching without magnetic separation was sufficient to increase the brightness index from 78.2 to 90.2% and from 91.3 to 95.7% in the two samples from Equador (RN and from 86.9 to 90.4% in the sample from Junco do Seridó (PB. The magnetic separation, although causing a small increase in the brightness index, was inefficient for removal of iron oxides and hydroxides. The results indicated no need for using magnetic separation in plants for beneficiation of kaolin from these two locations, providing the reduction of production costs allowing better exploitation of the ore.

  18. Experimentally studied laser fluorescence method for remote sensing of plant stress situation induced by improper plants watering

    Directory of Open Access Journals (Sweden)

    Yu. V. Fedotov

    2014-01-01

    Full Text Available Stressful situations of plants can be caused by a lack of nutrients; mechanical damages; diseases; low or high temperatures; lack of illumination; insufficient or excess humidity of the soil; soil salinization; soil pollution by oil products or heavy metals; the increased acidity of the soil; use of pesticides, herbicides, insecticides, etc.At early stages it is often difficult to detect seemingly that the plants are in stressful situations caused by adverse external factors. However, the fluorescent analysis potentially allows detection of the stressful situations of plants by deformation of laser-induced fluorescence spectra. The paper conducts experimental investigations to learn the capabilities of the laser fluorescent method to monitor plant situations at 532nm wavelength of fluorescence excitation in the stressful situations induced by improper watering (at excess of moisture in the soil and at a lack of moisture.Researches of fluorescence spectra have been conducted using a created laboratory installation. As a source to excite fluorescence radiation the second harmonica of YAG:Nd laser is used. The subsystem to record fluorescence radiation is designed using a polychromator and a highly sensitive matrix detector with the amplifier of brightness.Experimental investigations have been conducted for fast-growing and unpretentious species of plants, namely different sorts of salad.Experimental studies of laser-induced fluorescence spectra of plants for 532nm excitement wavelength show that the impact of stressful factors on a plant due to the improper watering, significantly distorts a fluorescence spectrum of plants. Influence of a stressful factor can be shown as a changing profile of a fluorescence spectrum (an identifying factor, here, is a relationship of fluorescence intensities at two wavelengths, namely 685 nm and 740 nm or (and as a changing level of fluorescence that can be the basis for the laser method for monitoring the plant

  19. The influence of NBD fluorescent probe on model membranes containing POPC and DPPC.

    Science.gov (United States)

    Weng, Chi-Jung; Wu, Ju-Ping; Kuo, Ming-Yen; Hsueh, Ya-Wei

    2016-03-01

    To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C 6 -NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by 2 H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C 6 -NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C 6 -NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules.

  20. Labeling RNAs in Live Cells Using Malachite Green Aptamer Scaffolds as Fluorescent Probes.

    Science.gov (United States)

    Yerramilli, V Siddartha; Kim, Kyung Hyuk

    2018-03-16

    RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA), an RNA aptamer that can specifically bind to malachite green (MG) dye and induces it to emit far-red fluorescence signals. Previous studies on MGA showed a potential for the use of MGA for genetically tagging other RNA molecules in live cells. However, these studies also exhibited low fluorescence signals and high background noise. Here we constructed and tested RNA scaffolds containing multiple tandem repeats of MGA as a strategy to increase the brightness of the MGA aptamer-fluorogen system as well as to make the system fluoresce when tagging various RNA molecules, in live cells. We demonstrate that our MGA scaffolds can induce fluorescence signals by up to ∼20-fold compared to the basal level as a genetic tag for other RNA molecules. We also show that our scaffolds function reliably as genetically encoded fluorescent tags for mRNAs of fluorescent proteins and other RNA aptamers.

  1. Near infrared fluorescent chlorophyll nanoscale liposomes for sentinel lymph node mapping

    Science.gov (United States)

    Fan, Lina; Wu, Qiang; Chu, Maoquan

    2012-01-01

    Background Sentinel lymph node (SLN) mapping using in vivo near infrared fluorescence imaging has attracted great attention during the past few years. Here we report on the early use of poorly water-soluble chlorophyll with near infrared fluorescence extracted from the leaf of Chimonanthus salicifolius, for mouse axillary SLN mapping. Methods and results To improve the water solubility and SLN targeting of the chlorophyll, we encapsulated the chlorophyll in nanoscale liposomes. The liposome-coated chlorophyll nanocomposites obtained were spherical in shape and had an average diameter of 21.7 ± 6.0 nm. The nanocomposites dispersed well in water, and in aqueous suspension they exhibited brighter near infrared fluorescence than chlorophyll alone. After incubation of the nanocomposites with normal liver cells (QSG-7701) and macrophage cells (Ana-1) for no more than 48 hours, there was no obvious reduction in cell viability. When the nanocomposites were injected intradermally into the paw of a mouse, the axillary SLN was found to be strongly fluorescent and was easily visualized in real time without a requirement for surgery. The intensity of the near infrared fluorescence emitted by the SLN was obviously brighter than that emitted by the SLN of another mouse that had been intradermally injected with chlorophyll alone. Conclusion Our data show that the liposome-coated chlorophyll nanocomposites could have great potential for clinical SLN mapping due to their lack of toxicity, bright near infrared fluorescence, and small diameter. PMID:22787402

  2. Green Synthesis of Fluorescent Carbon Dots for Selective Detection of Tartrazine in Food Samples.

    Science.gov (United States)

    Xu, Hua; Yang, Xiupei; Li, Gu; Zhao, Chuan; Liao, Xiangjun

    2015-08-05

    A simple, economical, and green method for the preparation of water-soluble, high-fluorescent carbon quantum dots (C-dots) has been developed via hydrothermal process using aloe as a carbon source. The synthesized C-dots were characterized by atomic force microscope (AFM), transmission electron microscopy (TEM), fluorescence spectrophotometer, UV-vis absorption spectra as well as Fourier transform infrared spectroscopy (FTIR). The results reveal that the as-prepared C-dots were spherical shape with an average diameter of 5 nm and emit bright yellow photoluminescence (PL) with a quantum yield of approximately 10.37%. The surface of the C-dots was rich in hydroxyl groups and presented various merits including high fluorescent quantum yield, excellent photostability, low toxicity and satisfactory solubility. Additionally, we found that one of the widely used synthetic food colorants, tartrazine, could result in a strong fluorescence quenching of the C-dots through a static quenching process. The decrease of fluorescence intensity made it possible to determine tartrazine in the linear range extending from 0.25 to 32.50 μM, This observation was further successfully applied for the determination of tartrazine in food samples collected from local markets, suggesting its great potential toward food routine analysis. Results from our study may shed light on the production of fluorescent and biocompatible nanocarbons due to our simple and environmental benign strategy to synthesize C-dots in which aloe was used as a carbon source.

  3. High Brightness Neutron Source for Radiography. Final report

    International Nuclear Information System (INIS)

    Cremer, J.T.; Piestrup, Melvin A.; Gary, Charles K.; Harris, Jack L.; Williams, David J.; Jones, Glenn E.; Vainionpaa, J.H.; Fuller, Michael J.; Rothbart, George H.; Kwan, J.W.; Ludewigt, B.A.; Gough, R.A.; Reijonen, Jani; Leung, Ka-Ngo

    2008-01-01

    This research and development program was designed to improve nondestructive evaluation of large mechanical objects by providing both fast and thermal neutron sources for radiography. Neutron radiography permits inspection inside objects that x-rays cannot penetrate and permits imaging of corrosion and cracks in low-density materials. Discovering of fatigue cracks and corrosion in piping without the necessity of insulation removal is possible. Neutron radiography sources can provide for the nondestructive testing interests of commercial and military aircraft, public utilities and petrochemical organizations. Three neutron prototype neutron generators were designed and fabricated based on original research done at the Lawrence Berkeley National Laboratory (LBNL). The research and development of these generators was successfully continued by LBNL and Adelphi Technology Inc. under this STTR. The original design goals of high neutron yield and generator robustness have been achieved, using new technology developed under this grant. In one prototype generator, the fast neutron yield and brightness was roughly 10 times larger than previously marketed neutron generators using the same deuterium-deuterium reaction. In another generator, we integrate a moderator with a fast neutron source, resulting in a high brightness thermal neutron generator. The moderator acts as both conventional moderator and mechanical and electrical support structure for the generator and effectively mimics a nuclear reactor. In addition to the new prototype generators, an entirely new plasma ion source for neutron production was developed. First developed by LBNL, this source uses a spiral antenna to more efficiently couple the RF radiation into the plasma, reducing the required gas pressure so that the generator head can be completely sealed, permitting the possible use of tritium gas. This also permits the generator to use the deuterium-tritium reaction to produce 14-MeV neutrons with increases

  4. Benzothiadiazole Derivatives as Fluorescence Imaging Probes: Beyond Classical Scaffolds.

    Science.gov (United States)

    Neto, Brenno A D; Carvalho, Pedro H P R; Correa, Jose R

    2015-06-16

    as pure solids, no fading even after long periods of irradiation, bright emissions with no blinking, good signal-to-noise ratios, efficiency to transpose the cell membrane, and irradiation preferentially in the visible-light region are just some features noted by using BTDs. As the pioneering group in the use of fluorescent small-molecule BTDs for bioimaging purposes, we feel pleased to share our experience, results, advances, and personal perspectives with the readers of this Account. The readers will clearly note the huge advantages of using fluorescent BTDs over classical scaffolds, and hopefully they will be inspired and motivated to further BTD technology in the fields of molecular and cellular biology.

  5. Hi-C Observations of Penumbral Bright Dots

    Science.gov (United States)

    Alpert, S. E.; Tiwari, S. K.; Moore, R. L.; Savage, S. L.; Winebarger, A. R.

    2014-01-01

    We use high-quality data obtained by the High Resolution Coronal Imager (Hi-C) to examine bright dots (BDs) in a sunspot's penumbra. The sizes of these BDs are on the order of 1 arcsecond (1") and are therefore hard to identify using the Atmospheric Imaging Assembly's (AIA) 0.6" pixel(exp -1) resolution. These BD become readily apparent with Hi-C's 0.1" pixel(exp -1) resolution. Tian et al. (2014) found penumbral BDs in the transition region (TR) by using the Interface Region Imaging Spectrograph (IRIS). However, only a few of their dots could be associated with any enhanced brightness in AIA channels. In this work, we examine the characteristics of the penumbral BDs observed by Hi-C in a sunspot penumbra, including their sizes, lifetimes, speeds, and intensity. We also attempt to find any association of these BDs to the IRIS BDs. There are fewer Hi-C BDs in the penumbra than seen by IRIS, though different sunspots were studied. We use 193 Angstroms Hi-C data from July 11, 2012 which observed from approximately 18:52:00 UT- 18:56:00 UT and supplement it with data from AIA's 193 Angstrom passband to see the complete lifetime of the dots that were born before and/or lasted longer than Hi- C's 5-minute observation period. We use additional AIA passbands and compare the light curves of the BDs at different temperatures to test whether the Hi-C BDs are TR BDs. We find that most Hi-C BDs show clear movement, and of those that do, they move in a radial direction, toward or away from the sunspot umbra. Single BDs interact with other BDs, combining to fade away or brighten. The BDs that do not interact with other BDs tend to move less. Many of the properties of our BDs are similar to the extreme values of the IRIS BDs, e.g., they move slower on average and their sizes and lifetimes are on the higher end of the IRIS BDs. We infer that our penumbral BDs are the large-scale end of the distribution of BDs observed by IRIS.

  6. Transient events in bright debris discs: Collisional avalanches revisited

    Science.gov (United States)

    Thebault, P.; Kral, Q.

    2018-01-01

    Context. A collisional avalanche is set off by the breakup of a large planetesimal, releasing vast amounts of small unbound grains that enter a debris disc located further away from the star, triggering there a collisional chain reaction that could potentially create detectable transient structures. Aims: We investigate this mechanism, using for the first time a fully self-consistent code coupling dynamical and collisional evolutions. We also quantify for the first time the photometric evolution of the system and investigate whether or not avalanches could explain the short-term luminosity variations recently observed in some extremely bright debris discs. Methods: We use the state-of-the-art LIDT-DD code. We consider an avalanche-favoring A6V star, and two set-ups: a "cold disc" case, with a dust release at 10 au and an outer disc extending from 50 to 120 au, and a "warm disc" case with the release at 1 au and a 5-12 au outer disc. We explore, in addition, two key parameters: the density (parameterized by its optical depth τ) of the main outer disc and the amount of dust released by the initial breakup. Results: We find that avalanches could leave detectable structures on resolved images, for both "cold" and "warm" disc cases, in discs with τ of a few 10-3, provided that large dust masses (≳1020-5 × 1022 g) are initially released. The integrated photometric excess due to an avalanche is relatively limited, less than 10% for these released dust masses, peaking in the λ 10-20 μm domain and becoming insignificant beyond 40-50 μm. Contrary to earlier studies, we do not obtain stronger avalanches when increasing τ to higher values. Likewise, we do not observe a significant luminosity deficit, as compared to the pre-avalanche level, after the passage of the avalanche. These two results concur to make avalanches an unlikely explanation for the sharp luminosity drops observed in some extremely bright debris discs. The ideal configuration for observing an

  7. Fluorescing macerals from wood precursors

    Energy Technology Data Exchange (ETDEWEB)

    Stout, S A; Bensley, D F

    1987-01-01

    A preliminary investigation into the origin of wood-derived macerals has established the existence of autofluorescent maceral precursors in the secondary xylem of swamp-inhabiting plant species. The optical character and fluorescent properties of microtomed thin-sections of modern woods from the Florida Everglades and Okefenokee Swamp, Georgia are compared to the character and properties of their peatified equivalents from various Everglades and Okefenokee peat horizons and their lignitic equivalents from the Brandon lignite of Vermont and the Trail Ridge lignitic peat from northern Florida. The inherent fluorescence of woody cell walls is believed to be caused by lignin though other cell wall components may contribute. The fluorescence spectra for several wood and cell types had a ..gamma../sub m//sub a//sub x/ of 452 nm and Q value of 0.00. The color as observed in blue light and the spectral geometry as measured in UV light of peatified and lignitic woody cell walls (potential textinites) may change progressively during early coalification. Cell wall-derived maceral material is shown to maintain its fluorescing properties after being converted to a structureless material, perhaps a corpohuminite or humodetrinite precursor. Fluorescing xylem cell contents, such as condensed tannins or essential oils, can maintain the fluorescent character through early coalification. Xylem cell walls and xylem cell contents are shown to provide fluorescing progenitor materials which would not require subsequent infusion with 'lipid' materials to account for their fluorescence as phytoclast material or as macerals in coal. 35 references.

  8. Assessing Photosynthesis by Fluorescence Imaging

    Science.gov (United States)

    Saura, Pedro; Quiles, Maria Jose

    2011-01-01

    This practical paper describes a novel fluorescence imaging experiment to study the three processes of photochemistry, fluorescence and thermal energy dissipation, which compete during the dissipation of excitation energy in photosynthesis. The technique represents a non-invasive tool for revealing and understanding the spatial heterogeneity in…

  9. Molecular basis of photochromism of a fluorescent protein revealed by direct 13C detection under laser illumination

    International Nuclear Information System (INIS)

    Mizuno, Hideaki; Mal, Tapas Kumar; Waelchli, Markus; Fukano, Takashi; Ikura, Mitsuhiko; Miyawaki, Atsushi

    2010-01-01

    Dronpa is a green fluorescent protein homologue with a photochromic property. A green laser illumination reversibly converts Dronpa from a green-emissive bright state to a non-emissive dark state, and ultraviolet illumination converts it to the bright state. We have employed solution NMR to understand the underlying molecular mechanism of the photochromism. The detail characterization of Dronpa is hindered as it is metastable in the dark state and spontaneously converts to the bright state. To circumvent this issue, we have designed in magnet laser illumination device. By combining the device with a 150-mW argon laser at 514.5 nm, we have successfully converted and maintained Dronpa in the dark state in the NMR tube by continuous illumination during the NMR experiments. We have employed direct-detection of 13 C nuclei from the carbon skeleton of the chromophore for detailed characterization of chromophore in both states of Dronpa by using the Bruker TCI cryoprobe. The results from NMR data have provided direct evidence of the double bond formation between C α and C β of Y63 in the chromophore, the β-barrel structure in solution, and the ionized and protonated state of Y63 hydroxyl group in the bright and dark states, respectively. These studies have also revealed that a part of β-barrel around the chromophore becomes polymorphic only in the dark state, which may be critical to make the fluorescence dim by increasing the contribution of non-emissive vibrational relaxation pathways.

  10. ASAS-SN Discovery of a Bright Be Star Undergoing a Possible Outburst

    Science.gov (United States)

    Jayasinghe, T.; Stanek, K. Z.; Kochanek, C. S.; Thorstensen, J.; Rupert, J.; Prieto, J. L.; Shields, J. V.; Thompson, T. A.; Holoien, T. W.-S.; Shappee, B. J.; Dong, Subo

    2017-09-01

    As part of an ongoing effort by ASAS-SN project (Shappee et al. 2014; Kochanek et al. 2017) to characterize and catalog all bright variable stars (e.g., Jayasinghe et al. 2017, ATel #10634, #10677), we report the discovery of a bright Be star undergoing a possible outburst.

  11. Properties of bright solitons in averaged and unaveraged models for SDG fibres

    Science.gov (United States)

    Kumar, Ajit; Kumar, Atul

    1996-04-01

    Using the slowly varying envelope approximation and averaging over the fibre cross-section the evolution equation for optical pulses in semiconductor-doped glass (SDG) fibres is derived from the nonlinear wave equation. Bright soliton solutions of this equation are obtained numerically and their properties are studied and compared with those of the bright solitons in the unaveraged model.

  12. Short Pulse High Brightness X-ray Production with the PLEIADES Thomson Scattering Source

    International Nuclear Information System (INIS)

    Anderson, S.G.; Barty, C.P.J.; Betts, S.M.; Brown, W.J.; Crane, J.K.; Cross, R.R.; Fittinghoff, D.N.; Gibson, D.J.; Hartemann, F.V.; Kuba, J.; LaSage, G.P.; Rosenzweig, J.B.; Slaughter, D.R.; Springer, P.T.; Tremaine, A.M.

    2003-01-01

    We describe PLEIADES, a compact, tunable, high-brightness, ultra-short pulse, Thomson x-ray source. The peak brightness of the source is expected to exceed 10 20 photons/s/0.1% bandwidth/mm 2 /mrad 2 . Initial results are reported and compared to theoretical calculations

  13. T1 bright appendix sign to exclude acute appendicitis in pregnant women.

    Science.gov (United States)

    Shin, Ilah; An, Chansik; Lim, Joon Seok; Kim, Myeong-Jin; Chung, Yong Eun

    2017-08-01

    To evaluate the diagnostic value of the T1 bright appendix sign for the diagnosis of acute appendicitis in pregnant women. This retrospective study included 125 pregnant women with suspected appendicitis who underwent magnetic resonance (MR) imaging. The T1 bright appendix sign was defined as a high intensity signal filling more than half length of the appendix on T1-weighted imaging. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the T1 bright appendix sign for normal appendix identification were calculated in all patients and in those with borderline-sized appendices (6-7 mm). The T1 bright appendix sign was seen in 51% of patients with normal appendices, but only in 4.5% of patients with acute appendicitis. The overall sensitivity, specificity, PPV, and NPV of the T1 bright appendix sign for normal appendix diagnosis were 44.9%, 95.5%, 97.6%, and 30.0%, respectively. All four patients with borderline sized appendix with appendicitis showed negative T1 bright appendix sign. The T1 bright appendix sign is a specific finding for the diagnosis of a normal appendix in pregnant women with suspected acute appendicitis. • Magnetic resonance imaging is increasingly used in emergency settings. • Acute appendicitis is the most common cause of acute abdomen. • Magnetic resonance imaging is widely used in pregnant population. • T1 bright appendix sign can be a specific sign representing normal appendix.

  14. Nanopatterned yttrium aluminum garnet phosphor incorporated film for high-brightness GaN-based white light emitting diodes

    International Nuclear Information System (INIS)

    Cho, Joong-yeon; Park, Sang-Jun; Ahn, Jinho; Lee, Heon

    2014-01-01

    In this study, we fabricated high-brightness white light emitting diodes (LEDs) by developing a nanopatterned yttrium aluminum garnet (YAG) phosphor-incorporated film. White light can be obtained by mixing blue light from a GaN-based LED and yellow light of the YAG phosphor-incorporated film. If white light sources can be fabricated by exciting proper yellow phosphor using blue light, then these sources can be used instead of the conventional fluorescent lamps with a UV source, for backlighting of displays. In this work, a moth-eye structure was formed on the YAG phosphor-incorporated film by direct spin-on glass (SOG) printing. The moth-eye structures have been investigated to improve light transmittance in various optoelectronic devices, including photovoltaic solar cells, light emitting diodes, and displays, because of their anti-reflection property. Direct SOG printing, which is a simple, easy, and relatively inexpensive process, can be used to fabricate nanoscale structures. After direct SOG printing, the moth-eye structure with a diameter of 220 nm was formed uniformly on the YAG phosphor-incorporated film. As a result of moth-eye patterning on the YAG phosphor-incorporated film, the light output power of a white LED with a patterned YAG phosphor-incorporated film increased to up to 13% higher than that of a white LED with a non-patterned film. - Highlights: • GaN-based high-brightness white LED was prepared using patterned YAG phosphor-incorporated films. • Direct hydrogen silsesquioxane printing was used to form moth-eye patterns on the YAG films. • The electroluminescence intensity of the white LED was enhanced by up to 14.9%

  15. Nanopatterned yttrium aluminum garnet phosphor incorporated film for high-brightness GaN-based white light emitting diodes

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Joong-yeon; Park, Sang-Jun [Department of Materials Science and Engineering, Korea University, Seoul 136-713 (Korea, Republic of); Ahn, Jinho, E-mail: jhahn@hanyang.ac.kr [Department of Material Science and Engineering, Hanyang University, Seoul 133-791 (Korea, Republic of); Lee, Heon, E-mail: heonlee@korea.ac.kr [Department of Materials Science and Engineering, Korea University, Seoul 136-713 (Korea, Republic of)

    2014-11-03

    In this study, we fabricated high-brightness white light emitting diodes (LEDs) by developing a nanopatterned yttrium aluminum garnet (YAG) phosphor-incorporated film. White light can be obtained by mixing blue light from a GaN-based LED and yellow light of the YAG phosphor-incorporated film. If white light sources can be fabricated by exciting proper yellow phosphor using blue light, then these sources can be used instead of the conventional fluorescent lamps with a UV source, for backlighting of displays. In this work, a moth-eye structure was formed on the YAG phosphor-incorporated film by direct spin-on glass (SOG) printing. The moth-eye structures have been investigated to improve light transmittance in various optoelectronic devices, including photovoltaic solar cells, light emitting diodes, and displays, because of their anti-reflection property. Direct SOG printing, which is a simple, easy, and relatively inexpensive process, can be used to fabricate nanoscale structures. After direct SOG printing, the moth-eye structure with a diameter of 220 nm was formed uniformly on the YAG phosphor-incorporated film. As a result of moth-eye patterning on the YAG phosphor-incorporated film, the light output power of a white LED with a patterned YAG phosphor-incorporated film increased to up to 13% higher than that of a white LED with a non-patterned film. - Highlights: • GaN-based high-brightness white LED was prepared using patterned YAG phosphor-incorporated films. • Direct hydrogen silsesquioxane printing was used to form moth-eye patterns on the YAG films. • The electroluminescence intensity of the white LED was enhanced by up to 14.9%.

  16. Dual-emissive quantum dots for multispectral intraoperative fluorescence imaging.

    Science.gov (United States)

    Chin, Patrick T K; Buckle, Tessa; Aguirre de Miguel, Arantxa; Meskers, Stefan C J; Janssen, René A J; van Leeuwen, Fijs W B

    2010-09-01

    Fluorescence molecular imaging is rapidly increasing its popularity in image guided surgery applications. To help develop its full surgical potential it remains a challenge to generate dual-emissive imaging agents that allow for combined visible assessment and sensitive camera based imaging. To this end, we now describe multispectral InP/ZnS quantum dots (QDs) that exhibit a bright visible green/yellow exciton emission combined with a long-lived far red defect emission. The intensity of the latter emission was enhanced by X-ray irradiation and allows for: 1) inverted QD density dependent defect emission intensity, showing improved efficacies at lower QD densities, and 2) detection without direct illumination and interference from autofluorescence. Copyright 2010 Elsevier Ltd. All rights reserved.

  17. Time-resolved fluorescence spectroscopy

    International Nuclear Information System (INIS)

    Gustavsson, Thomas; Mialocq, Jean-Claude

    2007-01-01

    This article addresses the evolution in time of light emitted by a molecular system after a brief photo-excitation. The authors first describe fluorescence from a photo-physical point of view and discuss the characterization of the excited state. Then, they explain some basic notions related to fluorescence characterization (lifetime and decays, quantum efficiency, so on). They present the different experimental methods and techniques currently used to study time-resolved fluorescence. They discuss basic notions of time resolution and spectral reconstruction. They briefly present some conventional methods: intensified Ccd cameras, photo-multipliers and photodiodes associated with a fast oscilloscope, and phase modulation. Other methods and techniques are more precisely presented: time-correlated single photon counting (principle, examples, and fluorescence lifetime imagery), streak camera (principle, examples), and optical methods like the Kerr optical effect (principle and examples) and fluorescence up-conversion (principle and theoretical considerations, examples of application)

  18. SKY BRIGHTNESS AND TRANSPARENCY IN THE i-BAND AT DOME A, ANTARCTICA

    International Nuclear Information System (INIS)

    Zou Hu; Zhou Xu; Jiang Zhaoji; Hu Jingyao; Ma Jun; Ashley, M. C. B.; Luong-Van, D. M.; Storey, J. W. V.; Cui Xiangqun; Feng Longlong; Gong Xuefei; Kulesa, C. A.; Lawrence, J. S.; Liu Genrong; Moore, A. M.; Pennypacker, C. R.; Travouillon, T.; Qin Weijia; Sun Bo; Shang Zhaohui

    2010-01-01

    The i-band observing conditions at Dome A on the Antarctic plateau have been investigated using data acquired during 2008 with the Chinese Small Telescope Array. The sky brightness, variations in atmospheric transparency, cloud cover, and the presence of aurorae are obtained from these images. The median sky brightness of moonless clear nights is 20.5 mag arcsec -2 in the SDSS i band at the south celestial pole (which includes a contribution of about 0.06 mag from diffuse Galactic light). The median over all Moon phases in the Antarctic winter is about 19.8 mag arcsec -2 . There were no thick clouds in 2008. We model contributions of the Sun and the Moon to the sky background to obtain the relationship between the sky brightness and transparency. Aurorae are identified by comparing the observed sky brightness to the sky brightness expected from this model. About 2% of the images are affected by relatively strong aurorae.

  19. Tolerance of brightness and contrast adjustments on chronic apical abscess and apical granuloma interpretation

    Science.gov (United States)

    Purnamasari, L.; Iskandar, H. H. B.; Makes, B. N.

    2017-08-01

    In digitized radiography techniques, adjusting the image enhancement can improve the subjective image quality by optimizing the brightness and contrast for diagnostic needs. To determine the value range of image enhancement (brightness and contrast) on chronic apical abscess and apical granuloma interpretation. 30 periapical radiographs that diagnosed chronic apical abscess and 30 that diagnosed apical granuloma were adjusted by changing brightness and contrast values. The value range of brightness and contrast adjustment that can be tolerated in radiographic interpretations of chronic apical abscess and apical granuloma spans from -10 to +10. Brightness and contrast adjustments on digital radiographs do not affect the radiographic interpretation of chronic apical abscess and apical granuloma if conducted within the value range.

  20. High cortisol awakening response is associated with an impairment of the effect of bright light therapy

    DEFF Research Database (Denmark)

    Martiny, Klaus Per Juul; Lunde, Marianne Anita; Undén, M

    2009-01-01

    OBJECTIVE: We investigated the predictive validity of the cortisol awakening response (CAR) in patients with non-seasonal major depression. METHOD: Patients were treated with sertraline in combination with bright or dim light therapy for a 5-week period. Saliva cortisol levels were measured in 63...... patients, as an awakening profile, before medication and light therapy started. The CAR was calculated by using three time-points: awakening and 20 and 60 min after awakening. RESULTS: Patients with low CAR had a very substantial effect of bright light therapy compared with dim light therapy, whereas...... patients with a high CAR had no effect of bright light therapy compared with dim light therapy. CONCLUSION: High CAR was associated with an impairment of the effect of bright light therapy. This result raises the question of whether bright light acts through a mechanism different from...

  1. Tolerance of image enhancement brightness and contrast in lateral cephalometric digital radiography for Steiner analysis

    Science.gov (United States)

    Rianti, R. A.; Priaminiarti, M.; Syahraini, S. I.

    2017-08-01

    Image enhancement brightness and contrast can be adjusted on lateral cephalometric digital radiographs to improve image quality and anatomic landmarks for measurement by Steiner analysis. To determine the limit value for adjustments of image enhancement brightness and contrast in lateral cephalometric digital radiography for Steiner analysis. Image enhancement brightness and contrast were adjusted on 100 lateral cephalometric radiography in 10-point increments (-30, -20, -10, 0, +10, +20, +30). Steiner analysis measurements were then performed by two observers. Reliabilities were tested by the Interclass Correlation Coefficient (ICC) and significance tested by ANOVA or the Kruskal Wallis test. No significant differences were detected in lateral cephalometric analysis measurements following adjustment of the image enhancement brightness and contrast. The limit value of adjustments of the image enhancement brightness and contrast associated with incremental 10-point changes (-30, -20, -10, 0, +10, +20, +30) does not affect the results of Steiner analysis.

  2. Brightness measurement of an electron impact gas ion source for proton beam writing applications

    Energy Technology Data Exchange (ETDEWEB)

    Liu, N.; Santhana Raman, P. [Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore 117542 (Singapore); Department of Electrical and Computer Engineering, National University of Singapore, Singapore 117583 (Singapore); Xu, X.; Pang, R.; Kan, J. A. van, E-mail: phyjavk@nus.edu.sg [Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore 117542 (Singapore); Khursheed, A. [Department of Electrical and Computer Engineering, National University of Singapore, Singapore 117583 (Singapore)

    2016-02-15

    We are developing a high brightness nano-aperture electron impact gas ion source, which can create ion beams from a miniature ionization chamber with relatively small virtual source sizes, typically around 100 nm. A prototype source of this kind was designed and successively micro-fabricated using integrated circuit technology. Experiments to measure source brightness were performed inside a field emission scanning electron microscope. The total output current was measured to be between 200 and 300 pA. The highest estimated reduced brightness was found to be comparable to the injecting focused electron beam reduced brightness. This translates into an ion reduced brightness that is significantly better than that of conventional radio frequency ion sources, currently used in single-ended MeV accelerators.

  3. The surface brightness of 1550 galaxies in Fornax: automated galaxy surface photometry: Pt. 2

    International Nuclear Information System (INIS)

    Phillipps, S.; Disney, M.J.; Kibblewhite, E.J.; Cawson, M.G.M.

    1987-01-01

    A survey of a complete sample of galaxies in the region of the Fornax cluster is presented. Measurements with the Automatic Plate Measuring machine are used to derive the observed distribution of galaxy surface brightness for 1550 objects. Corrections for surface brightness dependent selection effects are then made in order to estimate the true distribution. It is found that the sample (with 16.6 ≤ Msub(APM) ≤ 19.1) is divided into two distinct populations. The 'normal' galaxies with extrapolated central surface brightness Ssub(x) ≤ 22.5 Bμ form a uniformly distributed background of field galaxies. Low surface brightness galaxies (Ssub(x) ≥ 22.5 Bμ), on the other hand, are strongly clumped about the cluster centre. There appear to be few low surface brightness field galaxies. (author)

  4. Cross-dimensional mapping of number, length and brightness by preschool children.

    Directory of Open Access Journals (Sweden)

    Maria Dolores de Hevia

    Full Text Available Human adults in diverse cultures, children, infants, and non-human primates relate number to space, but it is not clear whether this ability reflects a specific and privileged number-space mapping. To investigate this possibility, we tested preschool children in matching tasks where the dimensions of number and length were mapped both to one another and to a third dimension, brightness. Children detected variation on all three dimensions, and they reliably performed mappings between number and length, and partially between brightness and length, but not between number and brightness. Moreover, children showed reliably better mapping of number onto the dimension of length than onto the dimension of brightness. These findings suggest that number establishes a privileged mapping with the dimension of length, and that other dimensions, including brightness, can be mapped onto length, although less efficiently. Children's adeptness at number-length mappings suggests that these two dimensions are intuitively related by the end of the preschool years.

  5. The new world atlas of artificial night sky brightness.

    Science.gov (United States)

    Falchi, Fabio; Cinzano, Pierantonio; Duriscoe, Dan; Kyba, Christopher C M; Elvidge, Christopher D; Baugh, Kimberly; Portnov, Boris A; Rybnikova, Nataliya A; Furgoni, Riccardo

    2016-06-01

    Artificial lights raise night sky luminance, creating the most visible effect of light pollution-artificial skyglow. Despite the increasing interest among scientists in fields such as ecology, astronomy, health care, and land-use planning, light pollution lacks a current quantification of its magnitude on a global scale. To overcome this, we present the world atlas of artificial sky luminance, computed with our light pollution propagation software using new high-resolution satellite data and new precision sky brightness measurements. This atlas shows that more than 80% of the world and more than 99% of the U.S. and European populations live under light-polluted skies. The Milky Way is hidden from more than one-third of humanity, including 60% of Europeans and nearly 80% of North Americans. Moreover, 23% of the world's land surfaces between 75°N and 60°S, 88% of Europe, and almost half of the United States experience light-polluted nights.

  6. THE zCOSMOS 10k-BRIGHT SPECTROSCOPIC SAMPLE

    International Nuclear Information System (INIS)

    Lilly, Simon J.; Maier, Christian; Carollo, Marcella; Caputi, Karina; Le Brun, Vincent; Kneib, Jean-Paul; Le Fevre, Olivier; De la Torre, Sylvain; De Ravel, Loic; Mainieri, Vincenzo; Mignoli, Marco; Zamorani, Gianni; Bardelli, Sandro; Bolzonella, Micol; Coppa, Graziano; Scodeggio, Marco; Contini, Thierry; Renzini, Alvio; Bongiorno, Angela; Cucciati, Olga

    2009-01-01

    We present spectroscopic redshifts of a large sample of galaxies with I AB -1 , independent of redshift. The reliability of individual redshifts is described by a Confidence Class that has been empirically calibrated through repeat spectroscopic observations of over 600 galaxies. There is very good agreement between spectroscopic and photometric redshifts for the most secure Confidence Classes. For the less secure Confidence Classes, there is a good correspondence between the fraction of objects with a consistent photometric redshift and the spectroscopic repeatability, suggesting that the photometric redshifts can be used to indicate which of the less secure spectroscopic redshifts are likely right and which are probably wrong, and to give an indication of the nature of objects for which we failed to determine a redshift. Using this approach, we can construct a spectroscopic sample that is 99% reliable and which is 88% complete in the sample as a whole, and 95% complete in the redshift range 0.5 < z < 0.8. The luminosity and mass completeness levels of the zCOSMOS-bright sample of galaxies is also discussed.

  7. Advanced laser technologies for high-brightness photocathode electron gun

    International Nuclear Information System (INIS)

    Tomizawa, Hiromitsu

    2012-01-01

    A laser-excited photocathode RF gun is one of the most reliable high-brightness electron beam sources for XFELs. Several 3D laser shaping methods have been developed as ideal photocathode illumination sources at SPring-8 since 2001. To suppress the emittance growth caused by nonlinear space-charge forces, the 3D cylindrical UV-pulse was optimized spatially as a flattop and temporally as squarely stacked chirped pulses. This shaping system is a serial combination of a deformable mirror that adaptively shapes the spatial profile with a genetic algorithm and a UV-pulse stacker that consists of four birefringent α-BBO crystal rods for temporal shaping. Using this 3D-shaped pulse, a normalized emittance of 1.4 πmm mrad was obtained in 2006. Utilizing laser's Z-polarization, Schottky-effect-gated photocathode gun was proposed in 2006. The cathode work functions are reduced by a laser-induced Schottky effect. As a result of focusing a radially polarized laser pulse with a hollow lens in vacuum, the Z-field (Z-polarization) is generated at the cathode. (author)

  8. Chandra's Darkest Bright Star: not so Dark after All?

    Science.gov (United States)

    Ayres, Thomas R.

    2008-11-01

    The Chandra High Resolution camera (HRC) has obtained numerous short exposures of the ultraviolet (UV)-bright star Vega (α Lyrae; HD 172167: A0 V), to calibrate the response of the detector to out-of-band (non-X-ray) radiation. A new analysis uncovered a stronger "blue leak" in the imaging section (HRC-I) than reported in an earlier study of Vega based on a subset of the pointings. The higher count rate—a factor of nearly 2 above prelaunch estimates—raised the possibility that genuine coronal X-rays might lurk among the out-of-band events. Exploiting the broader point-spread function of the UV leak compared with soft X-rays identified an excess of counts centered on the target, technically at 3σ significance. A number of uncertainties, however, prevent a clear declaration of a Vegan corona. A more secure result would be within reach of a deep uninterrupted HRC-I pointing.

  9. Temporal intensity interferometry: photon bunching in three bright stars

    Science.gov (United States)

    Guerin, W.; Dussaux, A.; Fouché, M.; Labeyrie, G.; Rivet, J.-P.; Vernet, D.; Vakili, F.; Kaiser, R.

    2017-12-01

    We report the first intensity correlation measured with starlight since the historical experiments of Hanbury Brown and Twiss. The photon bunching g(2)(τ, r = 0), obtained in the photon-counting regime, was measured for three bright stars: α Boo, α CMi and β Gem. The light was collected at the focal plane of a 1-m optical telescope, transported by a multi-mode optical fibre, split into two avalanche photodiodes and correlated digitally in real time. For total exposure times of a few hours, we obtained contrast values around 2 × 10-3, in agreement with the expectation for chaotic sources, given the optical and electronic bandwidths of our set-up. Comparing our results with the measurement of Hanbury Brown et al. for α CMi, we argue for the timely opportunity to extend our experiments to measuring the spatial correlation function over existing and/or foreseen arrays of optical telescopes diluted over several kilometres. This would enable microarcsec long-baseline interferometry in the optical, especially in the visible wavelengths, with a limiting magnitude of 10.

  10. On Quasi-Periodic Brightness Variations of P Cygni

    Science.gov (United States)

    Kochiashvili, N.; Beradze, S.; Natsvlishvili, R.; Kochiashvili, I.; Vardosanidze, M.; Pannicke, A.

    2018-03-01

    Until recent decades, it was considered that all Luminous Blue Variables are single massive and high luminosity stars. Now for several of them a companion has been found. The opinion exists that P Cygni also has a companion with an orbital period of about seven years. In accordance with this hypothesis, a known powerful eruption occurred near the periastron point. P Cygni, as well as several other well-known Luminous Blue Variable (LBV) stars, is a so-called "Supernova Impostor" because it survived after a powerful outburst. However, there were cases during the last decade when a LBV star survived after a powerful giant eruption, and then after a few years, explode as a supernova. Because the real reason for the great eruption and characteristic light variability of LBV, including P Cygni, is not established yet, any kind of photometric and spectral observational data is very significant. We present the results of analysis of the long-term photometric observations of hypergiant P Cygni. On the basis of these data, different quasi-periodic brightness changes of the star were revealed.

  11. High Brightness HDR Projection Using Dynamic Freeform Lensing

    KAUST Repository

    Damberg, Gerwin

    2016-05-03

    Cinema projectors need to compete with home theater displays in terms of image quality. High frame rate and spatial resolution as well as stereoscopic 3D are common features today, but even the most advanced cinema projectors lack in-scene contrast and, more important, high peak luminance, both of which are essential perceptual attributes of images appearing realistic. At the same time, HDR image statistics suggest that the average image intensity in a controlled ambient viewing environment such as the cinema can be as low as 1% for cinematic HDR content and not often higher than 18%, middle gray in photography. Traditional projection systems form images and colors by blocking the source light from a lamp, therefore attenuating between 99% and 82% of light, on average. This inefficient use of light poses significant challenges for achieving higher peak brightness levels. In this work, we propose a new projector architecture built around commercially available components, in which light can be steered to form images. The gain in system efficiency significantly reduces the total cost of ownership of a projector (fewer components and lower operating cost), and at the same time increases peak luminance and improves black level beyond what is practically achievable with incumbent projector technologies. At the heart of this computational display technology is a new projector hardware design using phase modulation in combination with a new optimization algorithm that is capable of on-the-fly computation of freeform lens surfaces. © 2016 ACM.

  12. The First Post-Kepler Brightness Dips of KIC 8462852

    Science.gov (United States)

    Boyajian, Tabetha. S.; Alonso, Roi; Ammerman, Alex; Armstrong, David; Asensio Ramos, A.; Barkaoui, K.; Beatty, Thomas G.; Benkhaldoun, Z.; Benni, Paul; Bentley, Rory O.; Berdyugin, Andrei; Berdyugina, Svetlana; Bergeron, Serge; Bieryla, Allyson; Blain, Michaela G.; Capetillo Blanco, Alicia; Bodman, Eva H. L.; Boucher, Anne; Bradley, Mark; Brincat, Stephen M.; Brink, Thomas G.; Briol, John; Brown, David J. A.; Budaj, J.; Burdanov, A.; Cale, B.; Aznar Carbo, Miguel; Castillo García, R.; Clark, Wendy J.; Clayton, Geoffrey C.; Clem, James L.; Coker, Phillip H.; Cook, Evan M.; Copperwheat, Chris M.; Curtis, J. L.; Cutri, R. M.; Cseh, B.; Cynamon, C. H.; Daniels, Alex J.; Davenport, James R. A.; Deeg, Hans J.; De Lorenzo, Roberto; de Jaeger, Thomas; Desrosiers, Jean-Bruno; Dolan, John; Dowhos, D. J.; Dubois, Franky; Durkee, R.; Dvorak, Shawn; Easley, Lynn; Edwards, N.; Ellis, Tyler G.; Erdelyi, Emery; Ertel, Steve; Farfán, Rafael. G.; Farihi, J.; Filippenko, Alexei V.; Foxell, Emma; Gandolfi, Davide; Garcia, Faustino; Giddens, F.; Gillon, M.; González-Carballo, Juan-Luis; González-Fernández, C.; González Hernández, J. I.; Graham, Keith A.; Greene, Kenton A.; Gregorio, J.; Hallakoun, Na’ama; Hanyecz, Ottó; Harp, G. R.; Henry, Gregory W.; Herrero, E.; Hildbold, Caleb F.; Hinzel, D.; Holgado, G.; Ignácz, Bernadett; Ilyin, Ilya; Ivanov, Valentin D.; Jehin, E.; Jermak, Helen E.; Johnston, Steve; Kafka, S.; Kalup, Csilla; Kardasis, Emmanuel; Kaspi, Shai; Kennedy, Grant M.; Kiefer, F.; Kielty, C. L.; Kessler, Dennis; Kiiskinen, H.; Killestein, T. L.; King, Ronald A.; Kollar, V.; Korhonen, H.; Kotnik, C.; Könyves-Tóth, Réka; Kriskovics, Levente; Krumm, Nathan; Krushinsky, Vadim; Kundra, E.; Lachapelle, Francois-Rene; LaCourse, D.; Lake, P.; Lam, Kristine; Lamb, Gavin P.; Lane, Dave; Lau, Marie Wingyee; Lewin, Pablo; Lintott, Chris; Lisse, Carey; Logie, Ludwig; Longeard, Nicolas; Lopez Villanueva, M.; Whit Ludington, E.; Mainzer, A.; Malo, Lison; Maloney, Chris; Mann, A.; Mantero, A.; Marengo, Massimo; Marchant, Jon; Martínez González, M. J.; Masiero, Joseph R.; Mauerhan, Jon C.; McCormac, James; McNeely, Aaron; Meng, Huan Y. A.; Miller, Mike; Molnar, Lawrence A.; Morales, J. C.; Morris, Brett M.; Muterspaugh, Matthew W.; Nespral, David; Nugent, C. R.; Nugent, Katherine M.; Odasso, A.; O’Keeffe, Derek; Oksanen, A.; O’Meara, John M.; Ordasi, András; Osborn, Hugh; Ott, John J.; Parks, J. R.; Rodriguez Perez, Diego; Petriew, Vance; Pickard, R.; Pál, András; Plavchan, P.; Pollacco, Don; Pozo Nuñez, F.; Pozuelos, F. J.; Rau, Steve; Redfield, Seth; Relles, Howard; Ribas, Ignasi; Richards, Jon; Saario, Joonas L. O.; Safron, Emily J.; Sallai, J. Martin; Sárneczky, Krisztián; Schaefer, Bradley E.; Schumer, Clea F.; Schwartzendruber, Madison; Siegel, Michael H.; Siemion, Andrew P. V.; Simmons, Brooke D.; Simon, Joshua D.; Simón-Díaz, S.; Sitko, Michael L.; Socas-Navarro, Hector; Sódor, Á.; Starkey, Donn; Steele, Iain A.; Stone, Geoff; Strassmeier, Klaus G.; Street, R. A.; Sullivan, Tricia; Suomela, J.; Swift, J. J.; Szabó, Gyula M.; Szabó, Róbert; Szakáts, Róbert; Szalai, Tamás; Tanner, Angelle M.; Toledo-Padrón, B.; Tordai, Tamás; Triaud, Amaury H. M. J.; Turner, Jake D.; Ulowetz, Joseph H.; Urbanik, Marian; Vanaverbeke, Siegfried; Vanderburg, Andrew; Vida, Krisztián; Vietje, Brad P.; Vinkó, József; von Braun, K.; Waagen, Elizabeth O.; Walsh, Dan; Watson, Christopher A.; Weir, R. C.; Wenzel, Klaus; Westendorp Plaza, C.; Williamson, Michael W.; Wright, Jason T.; Wyatt, M. C.; Zheng, WeiKang; Zsidi, Gabriella

    2018-01-01

    We present a photometric detection of the first brightness dips of the unique variable star KIC 8462852 since the end of the Kepler space mission in 2013 May. Our regular photometric surveillance started in 2015 October, and a sequence of dipping began in 2017 May continuing on through the end of 2017, when the star was no longer visible from Earth. We distinguish four main 1%–2.5% dips, named “Elsie,” “Celeste,” “Skara Brae,” and “Angkor,” which persist on timescales from several days to weeks. Our main results so far are as follows: (i) there are no apparent changes of the stellar spectrum or polarization during the dips and (ii) the multiband photometry of the dips shows differential reddening favoring non-gray extinction. Therefore, our data are inconsistent with dip models that invoke optically thick material, but rather they are in-line with predictions for an occulter consisting primarily of ordinary dust, where much of the material must be optically thin with a size scale ≪1 μm, and may also be consistent with models invoking variations intrinsic to the stellar photosphere. Notably, our data do not place constraints on the color of the longer-term “secular” dimming, which may be caused by independent processes, or probe different regimes of a single process.

  13. Precision Statistical Analysis of Images Based on Brightness Distribution

    Directory of Open Access Journals (Sweden)

    Muzhir Shaban Al-Ani

    2017-07-01

    Full Text Available Study the content of images is considered an important topic in which reasonable and accurate analysis of images are generated. Recently image analysis becomes a vital field because of huge number of images transferred via transmission media in our daily life. These crowded media with images lead to highlight in research area of image analysis. In this paper, the implemented system is passed into many steps to perform the statistical measures of standard deviation and mean values of both color and grey images. Whereas the last step of the proposed method concerns to compare the obtained results in different cases of the test phase. In this paper, the statistical parameters are implemented to characterize the content of an image and its texture. Standard deviation, mean and correlation values are used to study the intensity distribution of the tested images. Reasonable results are obtained for both standard deviation and mean value via the implementation of the system. The major issue addressed in the work is concentrated on brightness distribution via statistical measures applying different types of lighting.

  14. Advanced Laser Technologies for High-brightness Photocathode Electron Gun

    Science.gov (United States)

    Tomizawa, Hiromitsu

    A laser-excited photocathode RF gun is one of the most reliable high-brightness electron beam sources for XFELs. Several 3D laser shaping methods have been developed as ideal photocathode illumination sources at SPring-8 since 2001. To suppress the emittance growth caused by nonlinear space-charge forces, the 3D cylindrical UV-pulse was optimized spatially as a flattop and temporally as squarely stacked chirped pulses. This shaping system is a serial combination of a deformable mirror that adaptively shapes the spatial profile with a genetic algorithm and a UV-pulse stacker that consists of four birefringent α-BBO crystal rods for temporal shaping. Using this 3D-shaped pulse, a normalized emittance of 1.4 π mm mrad was obtained in 2006. Utilizing laser's Z-polarization, Schottky-effect-gated photocathode gun was proposed in 2006. The cathode work functions are reduced by a laser-induced Schottky effect. As a result of focusing a radially polarized laser pulse with a hollow lens in vacuum, the Z-field (Z-polarization) is generated at the cathode.

  15. Low Cost Lithography Tool for High Brightness LED Manufacturing

    Energy Technology Data Exchange (ETDEWEB)

    Andrew Hawryluk; Emily True

    2012-06-30

    The objective of this activity was to address the need for improved manufacturing tools for LEDs. Improvements include lower cost (both capital equipment cost reductions and cost-ofownership reductions), better automation and better yields. To meet the DOE objective of $1- 2/kilolumen, it will be necessary to develop these highly automated manufacturing tools. Lithography is used extensively in the fabrication of high-brightness LEDs, but the tools used to date are not scalable to high-volume manufacturing. This activity addressed the LED lithography process. During R&D and low volume manufacturing, most LED companies use contact-printers. However, several industries have shown that these printers are incompatible with high volume manufacturing and the LED industry needs to evolve to projection steppers. The need for projection lithography tools for LED manufacturing is identified in the Solid State Lighting Manufacturing Roadmap Draft, June 2009. The Roadmap states that Projection tools are needed by 2011. This work will modify a stepper, originally designed for semiconductor manufacturing, for use in LED manufacturing. This work addresses improvements to yield, material handling, automation and throughput for LED manufacturing while reducing the capital equipment cost.

  16. Bright Soil Near 'McCool': Salty Deja Vu?

    Science.gov (United States)

    2006-01-01

    While driving eastward toward the northwestern flank of 'McCool Hill,' the wheels of NASA's Mars Exploration Rover Spirit churned up the largest amount of bright soil discovered so far in the mission. This image from Spirit's navigation camera, taken on the rover's 787th Martian day, or sol, of exploration (March 21, 2006), shows the strikingly light tone and large extent of the deposit. A few days earlier, Spirit's wheels unearthed a small patch of light-toned material informally named 'Tyrone.' In images from Spirit's panoramic camera, 'Tyrone' strongly resembled both 'Arad' and 'Paso Robles,' two patches of light-toned soils discovered earlier in the mission. Spirit found 'Paso Robles' in 2005 while climbing 'Cumberland Ridge' on the western slope of 'Husband Hill.' In early January 2006, the rover discovered 'Arad' on the basin floor just south of 'Husband Hill.' Spirit's instruments confirmed that those soils had a salty chemistry dominated by iron-bearing sulfates. Spirit's miniature thermal emission spectrometer is analyzing this most recent discovery, and researchers will compare it with those other deposits. These discoveries indicate that light-toned soil deposits might be widely distributed on the flanks and valley floors of the 'Columbia Hills' region in Gusev Crater on Mars. The salts may record the past presence of water, as they are easily mobilized and concentrated in liquid solution.

  17. Single Molecule Spectroscopy of Fluorescent Proteins

    NARCIS (Netherlands)

    Blum, Christian; Subramaniam, Vinod

    2009-01-01

    The discovery and use of fluorescent proteins has revolutionized cellular biology. Despite the widespread use of visible fluorescent proteins as reporters and sensors in cellular environments the versatile photophysics of fluorescent proteins is still subject to intense research. Understanding the

  18. Fluorescent multiplex cell flow systems and methods

    KAUST Repository

    Merzaban, Jasmeen; Abuelela, Ayman F.; Mohammad, Amal Jehad

    2017-01-01

    scanning system emits multiple electromagnetic wavelengths simultaneously it cause multiple fluorescent labels having different excitation wavelength maximums to fluoresce. The system can simultaneously capture real-time fluorescence images from at least

  19. Fabrication and imaging study of ultrasound/fluorescence bi-modal contrast agent based on polymeric microbubbles

    International Nuclear Information System (INIS)

    Xing Zhanwen; Ke Hengte; Wang Jinrui; Zhao Bo; Qu Enze; Yue Xiuli; Dai Zhifei

    2013-01-01

    Objective: To fabricate an ultrasound/fluorescence bi-modal contrast agent by encapsulating fluorescent quantum dots into polymeric ultrasound contrast agent microbubbles. Methods: Polylactic acid (PLA, 500 mg), (1R)-(+)-camphor (50 mg) and CdSe/ZnS quantum dots (0.5 ml, 2.3 μmol/L)were dissolved or dispersed in dichloromethane (10 ml) to form in an organic phase. Ammonium carbonate solution and poly (vinyl alcohol) solution were employed as the internal and external water phase, respectively. The fluorescent microbubbles were generated using double emulsion solvent evaporation and lyophilization methods. The morphology and illumination were characterized by scanning electron microscopy (SEM) and fluorescence spectrophotometry. Synchronized contrast-enhanced ultrasound and fluorescence imaging was acquired by injecting fluorescent microbubbles into the silicone tube coupled to a self-made ultrasound/fluorescence imaging device. Ultrasound/fluorescence bi-modal in vivo imaging was acquired on the kidney of New Zealand rabbits and suckling mice. Results: The fluorescent microbubbles were hollow spheres with an averaged diameter of (1.62 ± 1.47) μm. More than 99% of these microbubbles were less than 8 μm in diameter, which met the size criteria for ultrasound contrast agents. The fluorescence emission peak of the microbubbles appeared at 632 nm, indicating that good luminescence properties of quantum dots were maintained. In vitro ultrasound/fluorescence imaging showed no echoic signal when the silicone tube was filled with saline, but there was a strong echo when filled with fluorescent microbubbles. The liquid column with fluorescent microbubbles emitted red luminescence under ultraviolet irradiation. The kidney of the rabbit was remarkably enhanced after the administration of fluorescent microbubbles. Bright fluorescence could be observed at the injection site of the suckling mice via subcutaneous injection. Conclusions: A bi-modal but single contrast agent

  20. Fluorescent standards for photodynamic therapy

    Science.gov (United States)

    Belko, N.; Kavalenka, S.; Samtsov, M.

    2016-08-01

    Photodynamic therapy is an evolving technique for treatment of various oncological diseases. This method employs photosensitizers - species that lead to death of tumor cells after the photoactivation. For further development and novel applications of photodynamic therapy new photosensitizers are required. After synthesis of a new photosensitizer it is important to know its concentration in different biological tissues after its administration and distribution. The concentration is frequently measured by the extraction method, which has some disadvantages, e.g. it requires many biological test subjects that are euthanized during the measurement. We propose to measure the photosensitizer concentration in tissue by its fluorescence. For this purpose fluorescent standards were developed. The standards are robust and simple to produce; their fluorescence signal does not change with time. The fluorescence intensity of fluorescent standards seems to depend linearly on the dye concentration. A set of standards thus allow the calibration of a spectrometer. Finally, the photosensitizer concentration can be determined by the fluorescence intensity after comparing the corresponding spectrum with spectra of the set of fluorescent standards. A biological test subject is not euthanized during this kind of experiment. We hope this more humane technique can be used in future instead of the extraction method.

  1. Fluorescence molecular tomography in the presence of background fluorescence

    International Nuclear Information System (INIS)

    Soubret, Antoine; Ntziachristos, Vasilis

    2006-01-01

    Fluorescence molecular tomography is an emerging imaging technique that resolves the bio-distribution of engineered fluorescent probes developed for in vivo reporting of specific cellular and sub-cellular targets. The method can detect fluorochromes in picomole amounts or less, imaged through entire animals, but the detection sensitivity and imaging performance drop in the presence of background, non-specific fluorescence. In this study, we carried out a theoretical and an experimental investigation on the effect of background fluorescence on the measured signal and on the tomographic reconstruction. We further examined the performance of three subtraction methods based on physical models of photon propagation, using experimental data on phantoms and small animals. We show that the data pre-processing with subtraction schemes can improve image quality and quantification when non-specific background florescence is present

  2. Modulation of ICT probability in bi(polyarene)-based O-BODIPYs: towards the development of low-cost bright arene-BODIPY dyads.

    Science.gov (United States)

    Gartzia-Rivero, Leire; Sánchez-Carnerero, Esther M; Jiménez, Josue; Bañuelos, Jorge; Moreno, Florencio; Maroto, Beatriz L; López-Arbeloa, Iñigo; de la Moya, Santiago

    2017-09-12

    We report the synthesis, and spectroscopic and electrochemical properties of a selected library of novel spiranic O-BODIPYs bearing a phenol-based bi(polyarene) unit tethered to the boron center through oxygen atoms. These dyes constitute an interesting family of arene-BODIPY dyads useful for the development of photonic applications due to their synthetic accessibility and tunable photonic properties. It is demonstrated that the electron-donor capability of the involved arene moiety switches on a non-emissive intramolecular charge transfer (ICT) state, which restricts the fluorescence efficiency of the dyad. Interestingly, the influence of this non-radiative deactivation channel can be efficiently modulated by the substitution pattern, either at the dipyrrin ligand or at the polyarene moiety. Thus, dyads featuring electron-rich dipyrrin and electron-poor polyarene show lower or almost negligible ICT probability, and hence display bright fluorescence upon dual excitation at far-away spectral regions. This synthetic approach has allowed the easy development of low-cost efficient ultraviolet-absorbing visible-emitting cassettes by selecting properly the substitution pattern of the involved key units, dipyrrin and bi(polyarene), to modulate not only absorption and emission wavelengths, but also fluorescence efficiencies.

  3. High brightness diode lasers controlled by volume Bragg gratings

    Science.gov (United States)

    Glebov, Leonid

    2017-02-01

    Volume Bragg gratings (VBGs) recorded in photo-thermo-refractive (PTR) glass are holographic optical elements that are effective spectral and angular filters withstanding high power laser radiation. Reflecting VBGs are narrow-band spectral filters while transmitting VBGs are narrow-band angular filters. The use of these optical elements in external resonators of semiconductor lasers enables extremely resonant feedback that provides dramatic spectral and angular narrowing of laser diodes radiation without significant power and efficiency penalty. Spectral narrowing of laser diodes by reflecting VBGs demonstrated in wide spectral region from near UV to 3 μm. Commercially available VBGs have spectral width ranged from few nanometers to few tens of picometers. Efficient spectral locking was demonstrated for edge emitters (single diodes, bars, modules, and stacks), vertical cavity surface emitting lasers (VCSELs), grating coupled surface emitting lasers (GCSELs), and interband cascade lasers (ICLs). The use of multiplexed VBGs provides multiwavelength emission from a single emitter. Spectrally locked semiconductor lasers demonstrated CW power from milliwatts to a kilowatt. Angular narrowing by transmitting VBGs enables single transverse mode emission from wide aperture diode lasers having resonators with great Fresnel numbers. This feature provides close to diffraction limit divergence along a slow axis of wide stripe edge emitters. Radiation exchange between lasers by means of spatially profiled or multiplexed VBGs enables coherent combining of diode lasers. Sequence of VBGs or multiplexed VBGs enable spectral combining of spectrally narrowed diode lasers or laser modules. Thus the use of VBGs for diode lasers beam control provides dramatic increase of brightness.

  4. A high-brightness thermionic microwave electron gun

    International Nuclear Information System (INIS)

    Borland, M.

    1991-02-01

    In a collaborative effort by SSRL, AET Associates, and Varian Associates, a high-brightness microwave electron gun using a thermionic cathode has been designed, built, tested, and installed for use with the SSRL 150 MeV linear accelerator. This thesis discusses the physics behind the design and operation of the gun and associated systems, presenting predictions and experimental tests of the gun's performance. The microwave gun concept is of increasing interest due to its promise of providing higher-current, lower-emittance electron beams than possible from conventional, DC gun technology. In a DC guns, accelerating gradients are less than 8 MV/m, while those in a microwave gun can exceed 100 MV/m, providing much more rapid initial acceleration, thereby reducing the deleterious effects of space-charge. Microwave guns produce higher momentum beams than DC guns, thus lessening space-charge effects during subsequent beam transport. Typical DC guns produce kinetic energies of 80--400 KeV, compared to 2--3 MeV for the SSRL microwave gun. ''State-of-the-art'' microwave gun designs employ laser-driven photocathodes, providing excellent performance but with greater complexity and monetary costs. A thermionic microwave gun with a magnetic bunching system is comparable in cost and complexity to a conventional system, but provides performance that is orders of magnitude better. Simulations of the SSRL microwave gun predict a normalized RMS emittance at the gun exist of e c · μm for a beam consisting of approximately 50% of the particles emitted from the gun, and having a momentum spread ±10%. These emittances are for up to 5 x 10 9 e - per bunch. Chromatic aberrations in the transport line between the gun and linear accelerator increase this to typically e · μm

  5. A high-brightness thermionic microwave electron gun

    Energy Technology Data Exchange (ETDEWEB)

    Borland, Michael [Stanford Univ., CA (United States)

    1991-02-01

    In a collaborative effort by SSRL, AET Associates, and Varian Associates, a high-brightness microwave electron gun using a thermionic cathode has been designed, built, tested, and installed for use with the SSRL 150 MeV linear accelerator. This thesis discusses the physics behind the design and operation of the gun and associated systems, presenting predictions and experimental tests of the gun`s performance. The microwave gun concept is of increasing interest due to its promise of providing higher-current, lower-emittance electron beams than possible from conventional, DC gun technology. In a DC guns, accelerating gradients are less than 8 MV/m, while those in a microwave gun can exceed 100 MV/m, providing much more rapid initial acceleration, thereby reducing the deleterious effects of space-charge. Microwave guns produce higher momentum beams than DC guns, thus lessening space-charge effects during subsequent beam transport. Typical DC guns produce kinetic energies of 80--400 KeV, compared to 2--3 MeV for the SSRL microwave gun. ``State-of-the-art`` microwave gun designs employ laser-driven photocathodes, providing excellent performance but with greater complexity and monetary costs. A thermionic microwave gun with a magnetic bunching system is comparable in cost and complexity to a conventional system, but provides performance that is orders of magnitude better. Simulations of the SSRL microwave gun predict a normalized RMS emittance at the gun exist of < 10 π • mec • μm for a beam consisting of approximately 50% of the particles emitted from the gun, and having a momentum spread ±10%. These emittances are for up to 5 x 109e- per bunch. Chromatic aberrations in the transport line between the gun and linear accelerator increase this to typically < 30 π • me • μm.

  6. Projection displays and MEMS: timely convergence for a bright future

    Science.gov (United States)

    Hornbeck, Larry J.

    1995-09-01

    Projection displays and microelectromechanical systems (MEMS) have evolved independently, occasionally crossing paths as early as the 1950s. But the commercially viable use of MEMS for projection displays has been illusive until the recent invention of Texas Instruments Digital Light Processing TM (DLP) technology. DLP technology is based on the Digital Micromirror DeviceTM (DMD) microchip, a MEMS technology that is a semiconductor digital light switch that precisely controls a light source for projection display and hardcopy applications. DLP technology provides a unique business opportunity because of the timely convergence of market needs and technology advances. The world is rapidly moving to an all- digital communications and entertainment infrastructure. In the near future, most of the technologies necessary for this infrastrucutre will be available at the right performance and price levels. This will make commercially viable an all-digital chain (capture, compression, transmission, reception decompression, hearing, and viewing). Unfortunately, the digital images received today must be translated into analog signals for viewing on today's televisions. Digital video is the final link in the all-digital infrastructure and DLP technoogy provides that link. DLP technology is an enabler for digital, high-resolution, color projection displays that have high contrast, are bright, seamless, and have the accuracy of color and grayscale that can be achieved only by digital control. This paper contains an introduction to DMD and DLP technology, including the historical context from which to view their developemnt. The architecture, projection operation, and fabrication are presented. Finally, the paper includes an update about current DMD business opportunities in projection displays and hardcopy.

  7. Prognostic factors for acute encephalopathy with bright tree appearance.

    Science.gov (United States)

    Azuma, Junji; Nabatame, Shin; Nakano, Sayaka; Iwatani, Yoshiko; Kitai, Yukihiro; Tominaga, Koji; Kagitani-Shimono, Kuriko; Okinaga, Takeshi; Yamamoto, Takehisa; Nagai, Toshisaburo; Ozono, Keiichi

    2015-02-01

    To determine the prognostic factors for encephalopathy with bright tree appearance (BTA) in the acute phase through retrospective case evaluation. We recruited 10 children with encephalopathy who presented with BTA and classified them into 2 groups. Six patients with evident regression and severe psychomotor developmental delay after encephalopathy were included in the severe group, while the remaining 4 patients with mild mental retardation were included in the mild group. We retrospectively analyzed their clinical symptoms, laboratory data, and magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) findings. Patients in the severe group developed subsequent complications such as epilepsy and severe motor impairment. Univariate analysis revealed that higher maximum lactate dehydrogenase (LDH) levels (p=0.055) were a weak predictor of poor outcome. Maximum creatinine levels were significantly higher (p<0.05) and minimal platelet counts were significantly lower (p<0.05) in the severe group than in the mild group. Acute renal failure was not observed in any patient throughout the study. MRS of the BTA lesion during the BTA period showed elevated lactate levels in 5 children in the severe group and 1 child in the mild group. MRI performed during the chronic phase revealed severe brain atrophy in all patients in the severe group. Higher creatinine and LDH levels and lower platelet counts in the acute phase correlated with poor prognosis. Increased lactate levels in the BTA lesion during the BTA period on MRS may predict severe physical and mental disability. Copyright © 2014 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.

  8. Search for bright nearby M dwarfs with virtual observatory tools

    Energy Technology Data Exchange (ETDEWEB)

    Aberasturi, M.; Caballero, J. A.; Montesinos, B.; Gálvez-Ortiz, M. C.; Solano, E.; Martín, E. L. [Centro de Astrobiología (CSIC-INTA), Departamento de Astrofísica, P.O. Box 78, E-28691 Villanueva de la Cañada, Madrid (Spain)

    2014-08-01

    Using Virtual Observatory tools, we cross-matched the Carlsberg Meridian 14 and the 2MASS Point Source catalogs to select candidate nearby bright M dwarfs distributed over ∼25,000 deg{sup 2}. Here, we present reconnaissance low-resolution optical spectra for 27 candidates that were observed with the Intermediate Dispersion Spectrograph at the 2.5 m Isaac Newton Telescope (R≈ 1600). We derived spectral types from a new spectral index, R, which measures the ratio of fluxes at 7485-7015 Å and 7120-7150 Å. We also used VOSA, a Virtual Observatory tool for spectral energy distribution fitting, to derive effective temperatures and surface gravities for each candidate. The resulting 27 targets were M dwarfs brighter than J = 10.5 mag, 16 of which were completely new in the Northern hemisphere and 7 of which were located at less than 15 pc. For all of them, we also measured Hα and Na I pseudo-equivalent widths, determined photometric distances, and identified the most active stars. The targets with the weakest sodium absorption, namely, J0422+2439 (with X-ray and strong Hα emissions), J0435+2523, and J0439+2333, are new members in the young Taurus-Auriga star-forming region based on proper motion, spatial distribution, and location in the color-magnitude diagram, which reopens the discussion on the deficit of M2-4 Taurus stars. Finally, based on proper motion diagrams, we report on a new wide M dwarf binary system in the field, LSPM J0326+3929EW.

  9. Velocity bunching of high-brightness electron beams

    Directory of Open Access Journals (Sweden)

    S. G. Anderson

    2005-01-01

    Full Text Available Velocity bunching has been recently proposed as a tool for compressing electron beam pulses in modern high brightness photoinjector sources. This tool is familiar from earlier schemes implemented for bunching dc electron sources, but presents peculiar challenges when applied to high current, low emittance beams from photoinjectors. The main difficulty foreseen is control of emittance oscillations in the beam in this scheme, which can be naturally considered as an extension of the emittance compensation process at moderate energies. This paper presents two scenarios in which velocity bunching, combined with emittance control, is to play a role in nascent projects. The first is termed ballistic bunching, where the changing of relative particle velocities and positions occur in distinct regions, a short high gradient linac, and a drift length. This scenario is discussed in the context of the proposed ORION photoinjector. Simulations are used to explore the relationship between the degree of bunching, and the emittance compensation process. Experimental measurements performed at the UCLA Neptune Laboratory of the surprisingly robust bunching process, as well as accompanying deleterious transverse effects, are presented. An unanticipated mechanism for emittance growth in bends for highly momentum chirped beam was identified and studied in these experiments. The second scenario may be designated as phase space rotation, and corresponds closely to the recent proposal of Ferrario and Serafini. Its implementation for the compression of the electron beam pulse length in the PLEIADES inverse Compton scattering (ICS experiment at LLNL is discussed. It is shown in simulations that optimum compression may be obtained by manipulation of the phases in low gradient traveling wave accelerator sections. Measurements of the bunching and emittance control achieved in such an implementation at PLEIADES, as well as aspects of the use of velocity-bunched beam directly

  10. Dark-Bright Soliton Dynamics Beyond the Mean-Field Approximation

    Science.gov (United States)

    Katsimiga, Garyfallia; Koutentakis, Georgios; Mistakidis, Simeon; Kevrekidis, Panagiotis; Schmelcher, Peter; Theory Group of Fundamental Processes in Quantum Physics Team

    2017-04-01

    The dynamics of dark bright solitons beyond the mean-field approximation is investigated. We first examine the case of a single dark-bright soliton and its oscillations within a parabolic trap. Subsequently, we move to the setting of collisions, comparing the mean-field approximation to that involving multiple orbitals in both the dark and the bright component. Fragmentation is present and significantly affects the dynamics, especially in the case of slower solitons and in that of lower atom numbers. It is shown that the presence of fragmentation allows for bipartite entanglement between the distinguishable species. Most importantly the interplay between fragmentation and entanglement leads to the decay of each of the initial mean-field dark-bright solitons into fast and slow fragmented dark-bright structures. A variety of excitations including dark-bright solitons in multiple (concurrently populated) orbitals is observed. Dark-antidark states and domain-wall-bright soliton complexes can also be observed to arise spontaneously in the beyond mean-field dynamics. Deutsche Forschungsgemeinschaft (DFG) in the framework of the SFB 925 ``Light induced dynamics and control of correlated quantum systems''.

  11. T1 bright appendix sign to exclude acute appendicitis in pregnant women

    International Nuclear Information System (INIS)

    Shin, Ilah; An, Chansik; Lim, Joon Seok; Kim, Myeong-Jin; Chung, Yong Eun

    2017-01-01

    To evaluate the diagnostic value of the T1 bright appendix sign for the diagnosis of acute appendicitis in pregnant women. This retrospective study included 125 pregnant women with suspected appendicitis who underwent magnetic resonance (MR) imaging. The T1 bright appendix sign was defined as a high intensity signal filling more than half length of the appendix on T1-weighted imaging. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the T1 bright appendix sign for normal appendix identification were calculated in all patients and in those with borderline-sized appendices (6-7 mm). The T1 bright appendix sign was seen in 51% of patients with normal appendices, but only in 4.5% of patients with acute appendicitis. The overall sensitivity, specificity, PPV, and NPV of the T1 bright appendix sign for normal appendix diagnosis were 44.9%, 95.5%, 97.6%, and 30.0%, respectively. All four patients with borderline sized appendix with appendicitis showed negative T1 bright appendix sign. The T1 bright appendix sign is a specific finding for the diagnosis of a normal appendix in pregnant women with suspected acute appendicitis. (orig.)

  12. T1 bright appendix sign to exclude acute appendicitis in pregnant women

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Ilah; An, Chansik; Lim, Joon Seok; Kim, Myeong-Jin; Chung, Yong Eun [Yonsei University College of Medicine, Department of Radiology, Research Institute of Radiological Science, Severance Hospital, 50-1 Yonsei-ro, Seodaemun-gu, Seoul (Korea, Republic of)

    2017-08-15

    To evaluate the diagnostic value of the T1 bright appendix sign for the diagnosis of acute appendicitis in pregnant women. This retrospective study included 125 pregnant women with suspected appendicitis who underwent magnetic resonance (MR) imaging. The T1 bright appendix sign was defined as a high intensity signal filling more than half length of the appendix on T1-weighted imaging. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the T1 bright appendix sign for normal appendix identification were calculated in all patients and in those with borderline-sized appendices (6-7 mm). The T1 bright appendix sign was seen in 51% of patients with normal appendices, but only in 4.5% of patients with acute appendicitis. The overall sensitivity, specificity, PPV, and NPV of the T1 bright appendix sign for normal appendix diagnosis were 44.9%, 95.5%, 97.6%, and 30.0%, respectively. All four patients with borderline sized appendix with appendicitis showed negative T1 bright appendix sign. The T1 bright appendix sign is a specific finding for the diagnosis of a normal appendix in pregnant women with suspected acute appendicitis. (orig.)

  13. Music for a Brighter World: Brightness Judgment Bias by Musical Emotion.

    Directory of Open Access Journals (Sweden)

    Joydeep Bhattacharya

    Full Text Available A prevalent conceptual metaphor is the association of the concepts of good and evil with brightness and darkness, respectively. Music cognition, like metaphor, is possibly embodied, yet no study has addressed the question whether musical emotion can modulate brightness judgment in a metaphor consistent fashion. In three separate experiments, participants judged the brightness of a grey square that was presented after a short excerpt of emotional music. The results of Experiment 1 showed that short musical excerpts are effective emotional primes that cross-modally influence brightness judgment of visual stimuli. Grey squares were consistently judged as brighter after listening to music with a positive valence, as compared to music with a negative valence. The results of Experiment 2 revealed that the bias in brightness judgment does not require an active evaluation of the emotional content of the music. By applying a different experimental procedure in Experiment 3, we showed that this brightness judgment bias is indeed a robust effect. Altogether, our findings demonstrate a powerful role of musical emotion in biasing brightness judgment and that this bias is aligned with the metaphor viewpoint.

  14. Music for a Brighter World: Brightness Judgment Bias by Musical Emotion.

    Science.gov (United States)

    Bhattacharya, Joydeep; Lindsen, Job P

    2016-01-01

    A prevalent conceptual metaphor is the association of the concepts of good and evil with brightness and darkness, respectively. Music cognition, like metaphor, is possibly embodied, yet no study has addressed the question whether musical emotion can modulate brightness judgment in a metaphor consistent fashion. In three separate experiments, participants judged the brightness of a grey square that was presented after a short excerpt of emotional music. The results of Experiment 1 showed that short musical excerpts are effective emotional primes that cross-modally influence brightness judgment of visual stimuli. Grey squares were consistently judged as brighter after listening to music with a positive valence, as compared to music with a negative valence. The results of Experiment 2 revealed that the bias in brightness judgment does not require an active evaluation of the emotional content of the music. By applying a different experimental procedure in Experiment 3, we showed that this brightness judgment bias is indeed a robust effect. Altogether, our findings demonstrate a powerful role of musical emotion in biasing brightness judgment and that this bias is aligned with the metaphor viewpoint.

  15. Arctic sea ice signatures: L-band brightness temperature sensitivity comparison using two radiation transfer models

    Directory of Open Access Journals (Sweden)

    F. Richter

    2018-03-01

    Full Text Available Sea ice is a crucial component for short-, medium- and long-term numerical weather predictions. Most importantly, changes of sea ice coverage and areas covered by thin sea ice have a large impact on heat fluxes between the ocean and the atmosphere. L-band brightness temperatures from ESA's Earth Explorer SMOS (Soil Moisture and Ocean Salinity have been proven to be a valuable tool to derive thin sea ice thickness. These retrieved estimates were already successfully assimilated in forecasting models to constrain the ice analysis, leading to more accurate initial conditions and subsequently more accurate forecasts. However, the brightness temperature measurements can potentially be assimilated directly in forecasting systems, reducing the data latency and providing a more consistent first guess. As a first step towards such a data assimilation system we studied the forward operator that translates geophysical parameters provided by a model into brightness temperatures. We use two different radiative transfer models to generate top of atmosphere brightness temperatures based on ORAP5 model output for the 2012/2013 winter season. The simulations are then compared against actual SMOS measurements. The results indicate that both models are able to capture the general variability of measured brightness temperatures over sea ice. The simulated brightness temperatures are dominated by sea ice coverage and thickness changes are most pronounced in the marginal ice zone where new sea ice is formed. There we observe the largest differences of more than 20 K over sea ice between simulated and observed brightness temperatures. We conclude that the assimilation of SMOS brightness temperatures yields high potential for forecasting models to correct for uncertainties in thin sea ice areas and suggest that information on sea ice fractional coverage from higher-frequency brightness temperatures should be used simultaneously.

  16. Arctic sea ice signatures: L-band brightness temperature sensitivity comparison using two radiation transfer models

    Science.gov (United States)

    Richter, Friedrich; Drusch, Matthias; Kaleschke, Lars; Maaß, Nina; Tian-Kunze, Xiangshan; Mecklenburg, Susanne

    2018-03-01

    Sea ice is a crucial component for short-, medium- and long-term numerical weather predictions. Most importantly, changes of sea ice coverage and areas covered by thin sea ice have a large impact on heat fluxes between the ocean and the atmosphere. L-band brightness temperatures from ESA's Earth Explorer SMOS (Soil Moisture and Ocean Salinity) have been proven to be a valuable tool to derive thin sea ice thickness. These retrieved estimates were already successfully assimilated in forecasting models to constrain the ice analysis, leading to more accurate initial conditions and subsequently more accurate forecasts. However, the brightness temperature measurements can potentially be assimilated directly in forecasting systems, reducing the data latency and providing a more consistent first guess. As a first step towards such a data assimilation system we studied the forward operator that translates geophysical parameters provided by a model into brightness temperatures. We use two different radiative transfer models to generate top of atmosphere brightness temperatures based on ORAP5 model output for the 2012/2013 winter season. The simulations are then compared against actual SMOS measurements. The results indicate that both models are able to capture the general variability of measured brightness temperatures over sea ice. The simulated brightness temperatures are dominated by sea ice coverage and thickness changes are most pronounced in the marginal ice zone where new sea ice is formed. There we observe the largest differences of more than 20 K over sea ice between simulated and observed brightness temperatures. We conclude that the assimilation of SMOS brightness temperatures yields high potential for forecasting models to correct for uncertainties in thin sea ice areas and suggest that information on sea ice fractional coverage from higher-frequency brightness temperatures should be used simultaneously.

  17. Instructive for disposal of fluorescent

    International Nuclear Information System (INIS)

    Salazar Vargas, Gerlin

    2014-01-01

    An instructive is established for the management system of waste fluorescent lamps, ensuring the storage, collection, transportation, and final disposal. The lamp is changed by an official of the Seccion de Matenimiento Construccion of the Oficina de Servicios Generales or is produced with the support of an official of the unit. The fluorescent should be deposited in stock of materials of the building maintenance section or unit specified with the help of a staff and in appropriate conditions. The fluorescent lamp is transported according to the guidelines in the manual. A responsible company is contracted by la Vicerrectoria de Administracion of the Universidad de Costa Rica dedicated to the transport and proper handling of fluorescent lamps [es

  18. ANTAGONISTIC POTENTIAL OF FLUORESCENT Pseudomonas ...

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    GROWTH OF TOMATO CHALLENGED WITH PHTOPATHOGENS ... This study focused on the antagonistic potential of fluorescent Pseudomonas in vitro, and its inoculation effect on growth .... the 5 days old culture in starch agar with Lugol's.

  19. Electron Source Brightness and Illumination Semi-Angle Distribution Measurement in a Transmission Electron Microscope.

    Science.gov (United States)

    Börrnert, Felix; Renner, Julian; Kaiser, Ute

    2018-05-21

    The electron source brightness is an important parameter in an electron microscope. Reliable and easy brightness measurement routes are not easily found. A determination method for the illumination semi-angle distribution in transmission electron microscopy is even less well documented. Herein, we report a simple measurement route for both entities and demonstrate it on a state-of-the-art instrument. The reduced axial brightness of the FEI X-FEG with a monochromator was determined to be larger than 108 A/(m2 sr V).

  20. Brightness of the photosphere and faculae at the limb based on eclipse observations

    Energy Technology Data Exchange (ETDEWEB)

    Akimov, L.A.; Belkina, I.L.; Dyatel, N.P.

    1982-05-01

    The absolute distributions of integral and surface brightness of the photospheric continuum (lambdaroughly-equal5870 A) and in faculae at the very limb are obtained from slitless spectrograms of the total solar eclipse of July 10, 1972. Several possible reasons for the brightness increase toward the limb in the distribution of photospheric surface brightness are discussed. The faculae showed high contrasts, up to 1.76 at a height of 200 km from the limb. A comparison of the times of local contacts observed and calculated with allowance for lunar relief showed that the active regions are at about 300 km above the photosphere. A schematic model of a facula is proposed.