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Sample records for breadfruit artocarpus altilis

  1. New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing1

    Science.gov (United States)

    De Bellis, Fabien; Malapa, Roger; Kagy, Valérie; Lebegin, Stéphane; Billot, Claire; Labouisse, Jean-Pierre

    2016-01-01

    Premise of the study: Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars. Methods and Results: A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single-locus markers were selected and assessed using 41 samples (39 A. altilis, one A. camansi, and one A. heterophyllus). All loci were polymorphic in A. altilis, 44 in A. camansi, and 21 in A. heterophyllus. The number of alleles per locus ranged from two to 19. Conclusions: The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management. PMID:27610273

  2. New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing.

    Science.gov (United States)

    De Bellis, Fabien; Malapa, Roger; Kagy, Valérie; Lebegin, Stéphane; Billot, Claire; Labouisse, Jean-Pierre

    2016-08-01

    Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars. A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single-locus markers were selected and assessed using 41 samples (39 A. altilis, one A. camansi, and one A. heterophyllus). All loci were polymorphic in A. altilis, 44 in A. camansi, and 21 in A. heterophyllus. The number of alleles per locus ranged from two to 19. The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management.

  3. Isolation and identification of mosquito (Aedes aegypti) biting deterrent fatty acids from male inflorescences of breadfruit (Artocarpus altilis (Parkinson)Fosberg)

    Science.gov (United States)

    Dried male inflorescences of breadfruit (Artocarpus altilis, Moraceae) are burned in communities throughout Oceania to repel flying insects, including mosquitoes. This study was conducted to identify chemicals responsible for mosquito deterrence. Various crude extracts were evaluated, and the most a...

  4. In vitro conservation and sustained production of breadfruit ( Artocarpus altilis, Moraceae): modern technologies for a traditional tropical crop

    Science.gov (United States)

    Murch, Susan J.; Ragone, Diane; Shi, Wendy Lei; Alan, Ali R.; Saxena, Praveen K.

    2008-02-01

    Breadfruit ( Artocarpus altilis, Moraceae) is a traditionally cultivated, high-energy, high-yield crop, but widespread use of the plant for food is limited by poor quality and poor storage properties of the fruit. A unique field genebank of breadfruit species and cultivars exists at the National Tropical Botanical Garden in the Hawaiian Islands and is an important global resource for conservation and sustainable use of breadfruit. However, this plant collection could be damaged by a random natural disaster such as a hurricane. We have developed a highly efficient in vitro plant propagation system to maintain, conserve, mass propagate, and distribute elite varieties of this important tree species. Mature axillary shoot buds were collected from three different cultivars of breadfruit and proliferated using a cytokinin-supplemented medium. The multiple shoots were maintained as stock cultures and repeatedly used to develop whole plants after root differentiation on a basal or an auxin-containing medium. The plantlets were successfully grown under greenhouse conditions and were reused to initiate additional shoot cultures for sustained production of plants. Flow cytometry was used to determine the nuclear deoxyribonucleic acid content and the ploidy status of the in vitro grown population. The efficacy of the micropropagation protocols developed in this study represents a significant advancement in the conservation and sustained mass propagation of breadfruit germplasm in a controlled environment free from contamination.

  5. Hypolipidemic effect of aqueous extract from the leaves of Artocarpus altilis "breadfruit" in Rattus norvegicus induced hyperlipidemia

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    Julio Campos Florián

    2013-12-01

    Full Text Available The present study aimed to demonstrate the hypolipidemic activity of aqueous extract of leaves of breadfruit, Artocarpus altilis, in a model of acute hyperlipidemia induced with triton X-305, using Rattus norvegicus specimens males, mean weight 204.5 g, who were orally administered 0.05 g/100g and 0.2 g/100 g of aqueous extract of A. altilis; included a negative control group received physiological saline and hyperlipidemic positive control group. After 24 hours of administering the treatments were made measurements of the concentrations of serum cholesterol and triglycerides. Found significant reductions (p < 0.01 in both cholesterol and triglyceride levels relative to the positive control group. We also found a significant difference (p < 0.01 between triglyceride concentrations of the animals treated with both doses of the aqueous extract of A. altilis. Conclude that the aqueous extract from the leaves of A. altilis has hypolipidemic effect at the doses tested for the model of hyperlipidemia induced with triton X-305.

  6. Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species.

    Science.gov (United States)

    Witherup, Colby; Ragone, Diane; Wiesner-Hanks, Tyr; Irish, Brian; Scheffler, Brian; Simpson, Sheron; Zee, Francis; Zuberi, M Iqbal; Zerega, Nyree J C

    2013-07-01

    Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. • A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241), A. camansi (34), A. mariannensis (15), and A. altilis × mariannensis (64) samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426) samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. • These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit.

  7. Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species1

    Science.gov (United States)

    Witherup, Colby; Ragone, Diane; Wiesner-Hanks, Tyr; Irish, Brian; Scheffler, Brian; Simpson, Sheron; Zee, Francis; Zuberi, M. Iqbal; Zerega, Nyree J. C.

    2013-01-01

    • Premise of the study: Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. • Methods and Results: A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241), A. camansi (34), A. mariannensis (15), and A. altilis × mariannensis (64) samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426) samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. • Conclusions: These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit. PMID:25202565

  8. Development of Microsatellite Loci in Artocarpus altilis (Moraceae and Cross-Amplification in Congeneric Species

    Directory of Open Access Journals (Sweden)

    Colby Witherup

    2013-07-01

    Full Text Available Premise of the study: Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. Methods and Results: A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241, A. camansi (34, A. mariannensis (15, and A. altilis × mariannensis (64 samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426 samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. Conclusions: These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit.

  9. Utilización de harina de frutos y hojas del árbol del pan (Artocarpus altilis en la ceba de conejos Nueva Zelanda Blanco Utilization of fruit and leaf meals from breadfruit tree (Artocarpus altilis for fattening New Zealand White rabbits

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    Coralia S Leyva

    2012-12-01

    Full Text Available Se utilizaron 36 conejos de la raza Nueva Zelanda Blanco, de 35 días de edad y 506 g de peso vivo, con el objetivo de evaluar el efecto de tres dietas en los indicadores productivos y económicos de los animales durante la ceba. Las dietas consistieron en: 1 pienso comercial más forraje fresco de glycine (Neonotonia wightii, como control; 2 harina de frutos del árbol del pan (Artocarpus altilis más glycine; y 3 pienso integral compuesto por una mezcla de harina de frutos y hojas del árbol del pan, más vitaminas y minerales. Se realizó un análisis de varianza, según un diseño completamente aleatorizado, con tres tratamientos y cuatro repeticiones. Después de 90 días en ceba, el peso vivo al sacrificio fue de: 2 347, 2 223 y 2 127 g/conejo, respectivamente; la ganancia media, de 20, 19 y 18 g/día; y la viabilidad fue del 100% en todos los tratamientos. Económicamente, el pienso balanceado con harina de frutos y hojas del árbol del pan tuvo una utilidad de 1,05 USD/conejo cebado, el sistema de alimentación con harina de frutos del árbol del pan más follaje de glycine alcanzó utilidades de 0,89 USD/conejo cebado; mientras que el sistema control generó pérdidas económicas, debido a los altos costos de las materias primas que componían el pienso convencional. Se concluye que los sistemas de alimentación alternativos que emplean la harina de frutos y hojas del árbol del pan resultan económica y biológicamente apropiados para los conejos en cebaThirty-six New Zealand White rabbits, 35 days old and with 506 g of live weight were used, in order to evaluate the effects of three diets on the productive and economic indicators of the animals during fattening. The diets consisted in: 1 commercial concentrate feed plus fresh forage of perennial soybean (Neonotonia wightií, as control; 2 breadfruit (Artocarpus altilis meal plus perennial soybean and 3 integral concentrate feed composed by a mixture of fruit and leaf meal from the

  10. Modifikasi Pati Sukun (Artocarpus Altilis dengan Teknik Oksidasi Menggunakan Hidrogen Peroksida Tanpa Katalis

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    Catarina Sri Budiyati

    2016-07-01

    Full Text Available Salah satu sumber bahan pangan lokal yang belum banyak dimanfaatkan adalah buah sukun (Artocarpus Artilis yang cukup banyak terdapat di Indonesia. Akan tetapi, pemanfaatan buah sukun terkendala pada kurangnya daya simpan buah segar. Oleh karena itu, perlu dilakukan upaya untuk meningkatkan pemanfaatan buah sukun dengan mengolahnya menjadi produk yang lebih luwes dengan daya simpan yang lebih baik, yaitu menjadi pati sukun. Sifat-sifat pati sukun perlu diperbaiki agar dapat digunakan sebagai pengganti tepung terigu. Tujuan dari penelitian ini adalah untuk memodifikasi pati sukun dengan cara oksidasi menggunakan larutan hidrogen peroksida. Dalam penelitian ini dikaji pengaruh konsistensi luluhan, konsentrasi hidrogen peroksida, waktu, dan suhu terhadap karakteristik fisikokimia pati sukun teroksidasi yang dihasilkan dan ditentukan kondisi optimalnya. Hasil penelitian menunjukkan bahwa semua variable yang dikaji tersebut mempengaruhi reaksi oksidasi pati sukun. Oksidasi pada konsistensi luluhan 20%, konsentrasi hidrogen peroksida 2% dan suhu 50°C mampu menghasilkan nilai swelling power dan water solubility yang terbaik. Reaksi oksidasi tidak mengubah struktur dan morfologi pati sukun termodifikasi secara signifikan.   [Title: Modification of Breadfruit (Artocarpus altilis starch through non-catalytic hydrogen peroxide oxidation] One of local source of food that has not been intensively explored is breadfruit (Artocarpus Artilis. However, the utilization of breadfruit is limited by the poor storage properties of the fresh fruit. Therefore, efforts should be made to improve the utilization of breadfruit to process it into more flexible products with better storability, suach as breadfruit starch. Breadfruit starch properties need to be improved so that it can be used to substitute wheat flour. The aim of this study was to modify the breadfruit starch by oxidation using hydrogen peroxide solution. This work studied examined the influence of

  11. Bio-deterioration of breadfruit (Artocarpus Communis)

    African Journals Online (AJOL)

    Gaga E Tonukari

    species (Tindal, 1965.) Breadfruit is a fruit tree that is propagated with the root cuttings and the average age of bearing first crop is between 4-6 years. It produces its fruit up to 2-3 times in a year and the number of fruits produced is very high (Soetjipto and Lubis, 1981). The fruit is aromatic, rich in latex and can weigh 1-4 kg.

  12. THE ALLELOPATHIC EFFECT OF SHREDDED LEAVES OF ARTOCARPUS HETEROPHYLLUS AND A. ALTILIS ON VIGNA RADIATA L

    OpenAIRE

    Romilly Margaret Mendez* & Glaxy Ezekel.V

    2017-01-01

    In the present study, an attempt has been made to study the effect of Artocarpus heterophyllus Lam. and Artocarpus altilis (Parkinson) Fosberg on seed germination, seedling growth and total phenolic content of Vigna radiata L. The objective of this study is to assess the rate of germination, growth of the seedlings and the chlorophyll content of the cultivar seeds exposed to four concentrations (10 ppm, 1 ppm, 0.1 ppm and 0.01 ppm) of the leaf extracts of A. heterophyllus and A. altilis. I...

  13. Pemanfaatan Pasta Sukun (Artocarpus altilis pada Pembuatan Mi Kering

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    Novi Safriani

    2013-06-01

    Full Text Available The aim of this study was to determine the best treatment combination between the ratio of wheat flour and breadfruit pasta, and the combination of the drying temperature and time to produce dried noodles with good quality and preferred by consumers. The effect of the ratio of wheat flour and breadfruit pasta (70:30%, 60:40%, and 50:50%, and the combination of the drying temperature and time (60°C, 70 minutes and 70°C, 60 minutes on the quality of the dried noodles were investigated. The results showed that the best quality of the dried noodles based on the organoleptic and cooking quality test obtained from the combination of the treatment of ratio of wheat flour and breadfruit pasta = 70:30% and the combination of drying temperature and time = 700C for 60 minutes with the following characteristics: water content of 8,78%, fat content of 13,67%, protein content of 11.90%, ash content of 1,35%, and carbohydrate content of 65,22%. The organoleptic value of the best dried noodles before rehydration: color of 2,75; flavor of 2,83; texture of 2,73; whereas after the rehydration, the best dried noodles has organoleptic values: color of 2,81; flavor of 2,92; and taste of 2,77.

  14. Bio-deterioration of breadfruit (Artocarpus Communis) in storage ...

    African Journals Online (AJOL)

    The bio-deterioration of breadfruit in storage and its effects on the nutrient composition of the fruit was investigated at Ibadan, Southwestern Nigeria. Freshly dropped fruits were stored under laboratory conditions for a period of 9 days. Aspergillus niger, Rhizopus stolonifer, Botryodiplodia theobromae, Mycovellosiella fulva, ...

  15. Preparation of a Breadfruit Flour Bar.

    Science.gov (United States)

    Nochera, Carmen L; Ragone, Diane

    2016-05-20

    Breadfruit is a nutritious, high energy food with a low quantity of protein but excellent protein quality. It has the potential to be developed into desired products which will help increase its utilization and add value to the crop. The overall purposes of this investigation were to develop a portable, nutritious, ready-to-eat breadfruit product (bar), test the sensory qualities of the product, and evaluate the nutritional properties of the product. Flour made from the Micronesian variety, Meinpadahk ( Artocarpus altilis × Artocarpus mariannensis ), was utilized for the development of the breadfruit bar. Breadfruit is a rich source of fiber, vitamins such as vitamin C, minerals such as potassium, and phytochemicals such as flavonoids. Nutritional labeling indicates that the breadfruit bar is high in carbohydrates and low in fat, and sensory evaluation indicates that 81% of the panelists found the bar acceptable while 19% disliked the bar. The breadfruit bar can provide an appealing and inexpensive gluten-free food source based on locally available breadfruit.

  16. Artocarpus altilis (Parkinson) Fosberg Extracts and Geranyl Dihydrochalcone Inhibit STAT3 Activity in Prostate Cancer DU145 Cells.

    Science.gov (United States)

    Jeon, Yoon Jung; Jung, Seung-Nam; Chang, Hyeyoun; Yun, Jieun; Lee, Chang Woo; Lee, Joonku; Choi, Sangho; Nash, Oyekanmi; Han, Dong Cho; Kwon, Byoung-Mog

    2015-05-01

    Artocarpus altilis (Parkinson) Fosberg has traditionally been used in Indonesia for the treatment of liver cirrhosis, hypertension, and diabetes. In many other countries, it is used for the treatment of malaria, yellow fever, and dengue fever. It has been reported that A. altilis extracts have antiatherosclerotic and cytoprotective effects, but its molecular targets in tumor cells are not yet fully understood. The A. altilis extracts and the partially purified fraction have been shown to inhibit STAT3 activity and the phosphorylation of STAT3 in a dose-dependent manner. To identify the active components, a bioassay-guided isolation of the partially purified fraction resulted in the identification of a geranyl dihydrochalcone, CG901. Its chemical structure was established on the basis of spectroscopic evidence and comparison with published data. The partially purified fraction and the isolated a geranyl dihydrochalcone, CG901, down-regulated the expression of STAT3 target genes, induced apoptosis in DU145 prostate cancer cells via caspase-3 and PARP degradation, and inhibited tumor growth in human prostate tumor (DU145) xenograft initiation model. These results suggest that A. altilis could be a good natural source and that the isolated compound will be a potential lead molecule for developing novel therapeutics against STAT3-related diseases, including cancer and inflammation. Copyright © 2015 John Wiley & Sons, Ltd.

  17. Effect of Powder Leaf Breadfruit Disposals (Arthocarpus Altilis) in Oil Mandar District and Polman Against Cholesterol and Glucose Mice (Mus Musculus)

    Science.gov (United States)

    Mu'nisa, A.; Asmawati, A.; Farida, A.; FA, Fressy; Erni

    2018-01-01

    The purpose of this study was to determine the effect of powdered leaves of breadfruit (Arthocarpus altilis) on oil is mandated origin of the Polman glucose and cholesterol levels in mice (Mus musculus). This study comprised 4 treatments and each treatment consisted of 5 replicates, ie groups of mice were fed a standard (negative control); 2 groups: group of mice fed with standard and cholesterol feed (positive control); Group 3 that mice fed with standard and Selayar oil; and group 4: group of mice fed with standard and Mandar oil that has been given powdered leaves of breadfruit. Measurement of glucose and blood cholesterol levels in mice done 3 times ie 2 weeks after the adaptation period (phase 1), 2 weeks after administration of the oil (phase 2) and 2 weeks after feeding cholesterol (stage 3). Based on the analysis of data both cholesterol and glucose levels showed that in a group of 4 decreased glucose and cholesterol levels in stage 2 but at stage 3 an increase in the group of mice given only the oil while in the group of mice given the oil and powdered leaves of breadfruit indicate glucose levels and normal cholesterol. The conclusion of this study show that the addition of powdered leaves of breadfruit into cooking oil Mandar influential in glucose levels and normalize blood cholesterol levels in mice.

  18. Biometria e características físico-químicas da fruta-pão (Artocarpus altilis

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    Erick dos Anjos Bezerra

    2017-02-01

    Full Text Available A espécie Artocarpus altilis produz frutos popularmente conhecidos como fruta-pão. São climatéricos e contribuem com quantidades consideráveis de energia e fornecer os principais nutrientes da dieta. Podem ser consumidos cozidos, fritos ou torrados e em processos tecnológicos tendo potencial para ser empregados em biscoitos e comercializados como farinha. Na literatura são poucos os trabalhos que relatem as características desses frutos, principalmente da região Nordeste. Desta forma, objetivou-se avaliar a biometria e as características físico-químicas da fruta-pão coletada no município de Santa Rita, Paraíba, Brasil. Foram selecionados 30 frutos firmes em 3 estádios de maturação, com pedúnculo, casca verde e amarelada, látex presente e livres de lesões. Os frutos apresentaram em média diâmetro externo transversal e diâmetro externo longitudinal de 15,98 (± 0,12 e 13,11 (± 0,13 cm, respectivamente, bem como massa média de 1,52 (± 0,06 Kg e firmeza de 66,80 (± 1,13 N. Quanto a composição centesimal apresentou valores de umidade, cinzas, proteína, lipídeos e carboidrato de 68,76% (± 0,02, 0,61% (± 0,01, 1,73 ± (0,00, 0,55% (± 0,01 e 28,35%, respectivamente, valor energético 125,27 Kcal, teor de amido de 24,68% (± 0,39, °Brix 2,01 (± 0,01, pH 6,4 (±0,01, fenólicos 0,023% (± 0,02 de ácido tânico e Aw 0,993 (± 0,00. De acordo com os resultados das físico-químicas, os frutos estudados apresentaram grande potencial para serem explorados comercialmente pela indústria de alimentos.Biometrics and physical-chemical characteristics of breadfruit (Artocarpus altilisAbstract: The species Artocarpus altilis produces fruits popularly known as breadfruit. They are climacteric and contribute considerable amounts of energy and provide the main nutrients in the diet. They can be consumed cooked, fried or roasted and in technological processes having potential to be used in cookies and marketed as flour. In the

  19. Direct Ethanol Production from Breadfruit Starch (Artocarpus communis Forst. by Engineered Simultaneous Saccharification and Fermentation (ESSF using Microbes Consortium

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    Iftachul Farida

    2015-02-01

    Full Text Available Breadfruit (Artocarpus communis Forst. is one of sources for ethanol production, which has high starch content (89%. Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s 0.34 g ethanol/g substrate; and yield of biomass (Yx/s 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s 0.41 g ethanol/g substrate, and the yield of cell (Yx/s 0.09 g cell/g substrate.

  20. Efecto hipolipidémico del extracto acuoso de las hojas de Artocarpus altilis "árbol del pan" en Rattus norvegicus con hiperlipidemia inducida

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    Julio Campos Florián

    2013-01-01

    Full Text Available La presente investigación tuvo como objetivo demostrar la actividad hipolipidémica del extracto acuoso de las hojas del árbol del pan, Artocarpus altilis, en un modelo de hiperlipidemia aguda inducida con tritón X-305, utilizando como especímenes Rattus norvegicus machos, peso promedio 204,5 g, a los que se les administró por vía oral 0,05 g/100 g y 0,2 g/100 g del extracto acuoso de A. altilis; se incluyó un grupo control negativo que recibió solución salina fisiológica y un grupo control positivo hiperlipidémico. Luego de 24 horas de administrar los tratamientos, se realizaron las mediciones en suero de las concentraciones de colesterol y triglicéridos. Encontramos reducciones significativas (p < 0,01 tanto de las cifras de colesterol, como de triglicéridos en relación a las concentraciones obtenidas en el grupo control positivo. También encontramos diferencia significativa (p < 0,01 entre las concentraciones de triglicéridos de los animales tratados con las dos dosis del extracto acuoso de A. altilis. Concluimos que el extracto acuoso de las hojas de A. altilis presenta efecto hipolipidémico a las dosis ensayadas para el modelo de hiperlipidemia inducida con tritón X-305.

  1. Efecto hipolipidémico del extracto acuoso de las hojas de Artocarpus altilis "árbol del pan" en Rattus norvegicus con hiperlipidemia inducida

    OpenAIRE

    Julio Campos Florián

    2013-01-01

    La presente investigación tuvo como objetivo demostrar la actividad hipolipidémica del extracto acuoso de las hojas del árbol del pan, Artocarpus altilis, en un modelo de hiperlipidemia aguda inducida con tritón X-305, utilizando como especímenes Rattus norvegicus machos, peso promedio 204,5 g, a los que se les administró por vía oral 0,05 g/100 g y 0,2 g/100 g del extracto acuoso de A. altilis; se incluyó un grupo control negativo que recibió solución salina fisiológica y un grupo control po...

  2. Theoretical investigation on the bond dissociation enthalpies of phenolic compounds extracted from Artocarpus altilis using ONIOM(ROB3LYP/6-311++G(2df,2p):PM6) method

    Science.gov (United States)

    Thong, Nguyen Minh; Duong, Tran; Pham, Linh Thuy; Nam, Pham Cam

    2014-10-01

    Theoretical calculations have been performed to predict the antioxidant property of phenolic compounds extracted from Artocarpus altilis. The Osbnd H bond dissociation enthalpy (BDE), ionization energy (IE), and proton dissociation enthalpy (PDE) of the phenolic compounds have been computed. The ONIOM(ROB3LYP/6-311++G(2df,2p):PM6) method is able to provide reliable evaluation for the BDE(Osbnd H) in phenolic compounds. An important property of antioxidants is determined via the BDE(Osbnd H) of those compounds extracted from A. altilis. Based on the BDE(Osbnd H), compound 12 is considered as a potential antioxidant with the estimated BDE value of 77.3 kcal/mol in the gas phase.

  3. PEMANFAATAN TEPUNG SUKUN (Artocarpus altilis sp. PADA PEMBUATAN ANEKA KUDAPAN SEBAGAI ALTERNATIF MAKANAN BERGIZI UNTUK PMT-AS

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    Dewanti Putri Pratiwi

    2013-11-01

    Full Text Available ABSTRACTBreadfruit is valuable fruit and has a good of nutrient content, but its usage is limited by poor storage properties of fresh fruit. Processing into flour, can increase its utilization. The aim of this research was to utilize breadfruit flour in production of nutritious snack foods for School Supplementary Feeding Programme (PMT-AS. There were three products developed in this study; brownies, pia, and croquette. The research was conducted using experimental design. The proportions of breadfruit flour and wheat flour were different for each product, which brownies was formulated using 70:30, 80:20, 90:10, and 100:0; whereas pia and croquette was formulated by 50:50, 60:40, 70:30, and 80:20. Proximate analysis showed that 100 g brownies contained 409 kcal energy and 7.5 g protein, 100 g pia had 383 kcal energy and 6.7 g protein and croquette had the highest energy and protein content is 455 kcal and 9.9 g protein. In conclusion, all products were suitable as alternative snacks for PMT-AS. The products have met 300 kcal energy and 5 g protein per serving size. Considered also the cost of production, these products have fulfilled criteria to be used in PMT-AS.Keywords: breadfruit, PMT-AS, snacksABSTRAKSukun merupakan buah yang bernilai dan memiliki kandungan gizi yang baik, tetapi penggunaannya terbatas oleh penyimpanan ketika berbentuk buah segar. Sukun yang diubah ke dalam bentuk tepung dapat meningkatkan pemanfaatannya. Tujuan dari penelitian ini adalah memanfaatkan tepung sukun dalam pembuatan kudapan sebagai alternatif makanan bergizi untuk PMT-AS. Terdapat tiga produk dalam penelitian ini, yaitu brownies, pia, dan kroket. Penelitian ini menggunakan desain eksperimental. Perbandingan tepung sukun dan tepung terigu berbeda untuk masing-masing produk. Formulasi brownies adalah 70:39, 80:20, 90:10, dan 100:0, sedangkan formulasi pia dan kroket adalah 50:50, 60:40, 70:30, dan 80:20. Berdasarkan uji organoleptik diketahui bahwa produk

  4. Rheological, baking, and sensory properties of composite bread dough with breadfruit (Artocarpus communis Forst) and wheat flours.

    Science.gov (United States)

    Bakare, Adegoke H; Osundahunsi, Oluwatooyin F; Olusanya, Joseph O

    2016-07-01

    The rheological (Pasting, farinograph, and alveograph) properties of wheat flour (WF) replaced with breadfruit four (05-40%) was analyzed. Baking and sensory qualities of the resulting bread were evaluated. Differences in baking properties of loaves produced under laboratory and industrial conditions were analyzed with t-test, whereas ANOVA was used for other analyses. Peak and final viscosities in the composite blends (CB) ranged from 109.20 to 114.06 RVU and 111.86 to 134.40 RVU, respectively. Dough stability decreased from 9.15 to 0.78 min, whereas farinograph water absorption increased 59.7-65.9%. Alveograph curve configuration ratio increased from 1.27 to 7.39, whereas specific volume (Spv) of the loaves decreased from 2.96 to 1.32 cm(3)/g. The Spv of WF loaves were not significantly different (P > 0.05) from that of the 5% CB, whereas production conditions had no significant effects on absorbed water (t = 0.532, df = 18 P = 0.3005), weight loss during baking (t = 0.865, df = 18, P = 0.199), and Spv (t = 0.828, df = 14.17, P = 0.211). The sensory qualities of the 5% blend were not significantly different from the WF.

  5. PEMANFAATAN SENYAWA BIOAKTIF DARI EKSTRAK KULIT BATANG Artocarpus sp SEBAGAI INHIBITOR TIROSINASE PADA PIGMENTASI KULIT

    OpenAIRE

    Florentina Maria Titin Supriyanti

    2009-01-01

    Telah dilakukan penelitian tentang pemanfaatan senyawa bioaktif dari ekstrak kulit batang Artocarpus sp sebagai inhibitor tirosinase pada pigmentasi kulit. Pada penelitian ini digunakan tiga jenis Artocarpus, yaitu A. heterophyllus (nangka), A. altilis (sukun) dan A. communis (kluwih). Kajian difokuskan pada penentuan jenis Artocarpus apakah yang potensial dalam menginhibisi reaksi tirosinase, serta pelarut organik apakah yang mampu mengekstrak senyawa bioaktif tersebut secara optimum. Metode...

  6. Importance socioculturelle de Artocarpus altilis (Parkinson) Fosberg ...

    African Journals Online (AJOL)

    SARAH

    31 mars 2014 ... conducted to evaluate the economic value of the species and to clarify the parental relationship between the two local forms of ..... arecaceae, an understory palm used for roof thatching in the Peruvian Amazon. Economic. Botany 54 (3), 267–277. Goussanou AC, Tente B, Djègo J, Agbani P, Sinsin B,. 2011.

  7. Effects of enriched compost with efficient microorganisms on the germination of recalcitrant seeds of breadfruit (Parkinson Fosberg and Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Yánez Yánez Wilfrido

    2016-11-01

    Full Text Available The research was conducted at the Center for Research, Graduate Studies and Amazon Conservation (CIPA of the Amazon State University, Ecuador. The purpose was to assess the effect of enriched compost Germination, day and day’s emergency transplantation two species of tropical origin and breadfruit Theobroma cacao. The experimental field is located in the province of Napo, 16'-1 and S and 77º 36'' 58'y 76'' W, at an altitude of 556 meters, with temperature between 18 and 32oC, average annual precipitation above 3000mm and relative humidity between 87 and 89%. An experimental design with five treatments in a randomized complete block design with three replications was applied. Treatments consisted of compost (1 MS, (2 lactic acid bacteria, (3 commercial yeast, (4 a combination thereof and (5 only compost, witness. It was determined that the compost mixture (T4 as a whole, has the greatest effect on the parameters of the seeds. Germination was found that in two of the T3 and T4 treatments significantly decreased the number of days to germination, in relation to the witness, 33 days and 13 days Artocarpus altilis, Theobroma cacao. The number of emerged plants, increase in both species with the addition of EM, lactic acid bacteria and commercial yeast (T4. a reduction is also reported in the days to transplant species studied to be significant particularly in cocoa. It was also determined that most bioburden corresponded to bacteria.

  8. Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

    OpenAIRE

    Achmad Fuad Hafid; Chie Aoki-Utsubo; Adita Ayu Permanasari; Myrna Adianti; Lydia Tumewu; Aty Widyawaruyanti; Sri Puji Astuti Wahyuningsih; Tutik Sri Wahyuni; Maria Inge Lusida; Soetjipto; Hak Hotta

    2017-01-01

    Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells ...

  9. Karakteristik Cookies Berbahan Dasar Tepung Sukun (Artocarpus communis Bagi Anak Penderita Autis

    Directory of Open Access Journals (Sweden)

    Dede Sukandar

    2017-03-01

    Abstract Breadfruit (Artocarpus communis has been reported as a functional food ingredient for autistic children. This study aims to determine the level of A panelist on cookies breadfruit and various formulations compared to other flour-based cookies which include the effect of additives on the properties of chemistry, physics, power accept cookies breadfruit, calcium and phosphorus levels cookies breadfruit, breadfruit cookies and find out the most preferred if it meets quality standards according to SNI 01-2973-1992 cookies or not. Organoleptic test was conducted to determine the level of preference and acceptance panelist on cookies breadfruit compared with other flour-based cookies     (wheat, rice, sago and breadfruit cookies in a variety of formulation. The parameters used include, colour, frageance, texture, flavor and overall acceptance. Calcium level test performed using atomic absorption spectroscopy method at λ 422.7 nm and phosphorus levels using UV-Vis spectroscopy at λ 880 nm. The data were analyzed using analysis of variance anova and Duncan analysis. The test results showed Cookies breadfruit organoleptif have low enrollment compared to other flour cookies views of the average revenue flavor and general acceptance of cookies breadfruit, breadfruit cookies formulations can improve the physical properties (fragrance, flavor, color, and texture and acceptance of cookies breadfruit by panelists. An increase in the average value of A formulation compared breadfruit cookies before formulation. Cookies are breadfruit tersukai 718 formulations with soy milk additives. Quality breadfruit cookies tersukai 718 formulations in general accordance with SNI 01-2973-1992 except protein content and contained 8.05% Cu content with a small concentration (1.56 ppm. Cookies breadfruit tersukai have the highest levels of calcium and phosphorus compared with breadfruit flour and commercial cookies for autistic children so that the most preferred breadfruit cookies very

  10. artocarpus altilis proving its worth in toxic metal removal

    African Journals Online (AJOL)

    DR. AMINU

    2012-12-02

    Dec 2, 2012 ... INTRODUCTION. Research to ensure clean and healthy environment ... products as effective and cheap methods of isolating .... about 2h, diluted and thoroughly mixed with KBr ..... Concise inorganic chemistry, 5th edn.

  11. Dried fruit breadfruit slices by Refractive Window™ technique

    Directory of Open Access Journals (Sweden)

    Diego F. Tirado

    2016-01-01

    Full Text Available A large amount of products are dried due several reasons as preservation, weight reduction and improvement of stability. However, on the market are not offered low-cost and high quality products simultaneously. Although there are effective methods of dehydrating foods such as freeze drying, which preserves the flavor, color and vitamins, they are poor accessibility technologies. Therefore, alternative processes are required to be efficient and economical. The aim of this research was compare drying kinetics of sliced of breadfruit (Artocarpus communis using the technique of Refractive Window® (VR with the tray drying. To carry out this study, sliced of 1 and 2 mm thick were used. Refractive window drying was performed with the water bath temperature to 92 °C; and tray drying at 62 °C and an air velocity of 0.52 m/s. During the Refractive window drying technique, the moisture content reached the lower than tray drying levels. Similarly it happened with samples of 1 mm, which, having a smaller diameter reached lower moisture levels than samples 2 mm. The higher diffusivities were obtained during drying sliced VR 1 and 2 mm with coefficients of 6.13 and 3.90*10-9 m2/s respectively.

  12. Out of Borneo: biogeography, phylogeny and divergence date estimates of Artocarpus (Moraceae).

    Science.gov (United States)

    Williams, Evelyn W; Gardner, Elliot M; Harris, Robert; Chaveerach, Arunrat; Pereira, Joan T; Zerega, Nyree J C

    2017-03-01

    The breadfruit genus ( Artocarpus , Moraceae) includes valuable underutilized fruit tree crops with a centre of diversity in Southeast Asia. It belongs to the monophyletic tribe Artocarpeae, whose only other members include two small neotropical genera. This study aimed to reconstruct the phylogeny, estimate divergence dates and infer ancestral ranges of Artocarpeae, especially Artocarpus , to better understand spatial and temporal evolutionary relationships and dispersal patterns in a geologically complex region. To investigate the phylogeny and biogeography of Artocarpeae, this study used Bayesian and maximum likelihood approaches to analyze DNA sequences from six plastid and two nuclear regions from 75% of Artocarpus species, both neotropical Artocarpeae genera, and members of all other Moraceae tribes. Six fossil-based calibrations within the Moraceae family were used to infer divergence times. Ancestral areas and estimated dispersal events were also inferred. Artocarpeae, Artocarpus and four monophyletic Artocarpus subgenera were well supported. A late Cretaceous origin of the Artocarpeae tribe in the Americas is inferred, followed by Eocene radiation of Artocarpus in Asia, with the greatest diversification occurring during the Miocene. Borneo is reconstructed as the ancestral range of Artocarpus , with dozens of independent in situ diversification events inferred there, as well as dispersal events to other regions of Southeast Asia. Dispersal pathways of Artocarpus and its ancestors are proposed. Borneo was central in the diversification of the genus Artocarpus and probably served as the centre from which species dispersed and diversified in several directions. The greatest amount of diversification is inferred to have occurred during the Miocene, when sea levels fluctuated and land connections frequently existed between Borneo, mainland Asia, Sumatra and Java. Many species found in these areas have extant overlapping ranges, suggesting that sympatric

  13. Effect of supplementation of African breadfruit (Treculia africana ...

    African Journals Online (AJOL)

    African breadfruit (Treculia africana) hulls were supplemented at different levels with other organic food processing wastes (orange, plantain, cassava and soybean). Optimum supplementation of 40:60 (breadfruit hulls to each waste) was obtained. Proximate and mineral composition of the unsupplemented and the ...

  14. Chemical, Functional and Organoleptic Evaluation of African Breadfruit

    African Journals Online (AJOL)

    African breadfruit (Treculia africana Decne) seeds were parboiled and their kernels dried ... age were evaluated. .... macular degeneration, and cataract formation ... Heat treatment increased water ab- ..... prevention of cardiovascular disease.

  15. THE PRODUCTION OF BREADFRUIT FLOUR: EFFECT OF HEATER TEMPERATURE TO THE DRYING RATE AND TIME OF THE BREADFRUIT

    Directory of Open Access Journals (Sweden)

    Denni Kartika Sari

    2017-06-01

    Full Text Available The composition of mineral and vitamin from breadfruit is particularly known of having benefits compared to rice which is a main source of carbohydrate consumed by societies. The process of drying is one of the factors that affects foodstuffs quality. The aim of this research was to provide an understanding of drying phenomena from data experiment and discover the influence of drying air temperature to breadfruit drying time and rates. This research was conducted in several stages which are material preparation (breadfruit by through downsizing process, then weigh the material (breadfruit once every 5 minutes on each drying air temperature variations (50 ºC, 60 oC, 70 oC, and 80 oC. The research were conducted using breadfruit with the best drying time which is obtained at 60 0C for 100 minutes. The lowest water content obtained was 0.4%, while the highest drying rate was 0.00144 Kg2/m2.s at 80 ºC of temperature.

  16. Chemical and Sensory Evaluation of African Breadfruit ( Treculia ...

    African Journals Online (AJOL)

    The effect of aluminum sulphate (alum) and sodium sesquicarbonate (trona) on the chemical and sensory qualities of African breadfruit seeds (ABFS) was studied. ABFS were steeped in (0 – 2.0%) alum concentrations for 24 h. The seeds were parboiled, dehulled and then cooked with (0 – 2.0%) trona concentrations for 90 ...

  17. The Effects of Substituting Soyabean Meal for Breadfruit Meal on ...

    African Journals Online (AJOL)

    Increasing substitutions of soyabean meal for breadfruit meal in diets fed to Heterobranchus bidorsalis (♂) x Clarias gariepinus (♀) hybrid fingerlings led to weight increases in all dietary types with exception of diet G and higher growth induction in catfishes fed all diets with exception of diet B. Diets C had induced better ...

  18. Performance Evaluation of a Prototyped Breadfruit Seed Dehulling Machine

    Directory of Open Access Journals (Sweden)

    Nnamdi Anosike

    2016-05-01

    Full Text Available The drudgery involved in dehulling breadfruit seed by traditional methods has been highlighted as one of the major problems hindering the realization of the full potential of breadfruit as a field to food material. This paper describes a development in an African breadfruit seed dehulling machine with increased throughput of about 70% above reported machines. The machine consists of a 20 mm diameter shaft, carrying a spiral wound around its circumference (feeder. The feeder provides the required rotational motion and turns a circular disk that rotates against a fixed disk. The two disks can be adjusted to maintain a pre-determined gap for dehulling. An inbuilt drying unit reduces the moisture content of the breadfruit for easy separation of the cotyledon from the endosperm immediately after the dehulling process. The sifting unit that separates the shell from the seed is achieved in this design with an electric fan. The machine is design to run at a speed of 250 rpm with an electric motor as the prime mover. The dehulling efficiency up to 86% and breakage of less than 1.3% was obtained at a clearance setting of 12.4 mm between disks. A sifting efficiency of 100% was achieved. Based on the design diameter and clearance between the dehulling disks, the machine throughput was 216 kg/h with an electric power requirement of 1.207 kW.

  19. Antibacterial activity of Artocarpus heterophyllus.

    Science.gov (United States)

    Khan, M R; Omoloso, A D; Kihara, M

    2003-07-01

    The crude methanolic extracts of the stem and root barks, stem and root heart-wood, leaves, fruits and seeds of Artocarpus heterophyllus and their subsequent partitioning with petrol, dichloromethane, ethyl acetate and butanol gave fractions that exhibited a broad spectrum of antibacterial activity. The butanol fractions of the root bark and fruits were found to be the most active. None of the fractions were active against the fungi tested.

  20. Diversity of secondary metabolites from Genus Artocarpus (Moraceae

    Directory of Open Access Journals (Sweden)

    ALIEFMAN HAKIM

    2010-11-01

    Full Text Available Hakim A. 2010. The diversity of secondary metabolites from Genus Artocarpus (Moraceae. Nusantara Bioscience 2:146-156. Several species of the Artocarpus genus (Moraceae have been investigated their natural product. The secondary metabolites successfully being isolatad from Artocarpus genus consist of terpenoid, flavonoids, stilbenoid, arylbenzofuran, neolignan, and adduct Diels-Alder. Flavonoid group represent the compound which is the most found from Artocarpus plant. The flavonoids compound which are successfully isolated from Artocarpus plant consist of the varied frameworks like chalcone, flavanone, flavan-3-ol, simple flavone, prenylflavone, oxepinoflavone, pyranoflavone, dihydrobenzoxanthone, furanodihydrobenzoxanthone, pyranodihydrobenzoxanthone, quinonoxanthone, cyclopentenoxanthone, xanthonolide, dihydroxanthone.

  1. Swelling power and solubility of modified breadfruit flour using Lactobacillus plantarum

    Science.gov (United States)

    Norita Rahma, Istiana; Haris Pratama, Raja; Alfiyanti; Reynaldo Alwi, Deo; Astuti, Woro Indriani Setyo Tri; Hesti Wardhani, Dyah

    2017-11-01

    Breadfruit has the high nutritional and carbohydrate content which is comparable with wheat, so it is potential to be processed as the breadfruit flour. However, some studies showed the breadfruit flour has low swelling power (SP) and water solubility (WS) compared to wheat. Hence, the flour required modification. The main purpose of this research was to increase the value of SP and WS on breadfruit flour through a fermentation process. Sterile breadfruit flour (50 g) was suspended using sterile distilled water in a 250 ml glass beaker to obtain the flour concentration of 25%, 30%, 35%, 40% and 45% (g/ml). A certain concentration of L. plantarum was added (5, 7.5, 10, and 12.5%, ml/ml) then incubated at 30°C for 24, 48, 72, 96 and 120 h. The obtained solid cake was washed before being dried. The effect of cultural concentration on swelling power (SP) and water solubility (WS) is presented in Figure 1. The optimum fermentation condition was achieved by a 35% concentration of breadfruit flour which fermented with 10% L. plantarum for 24 h fermentation. At this condition, all parameter showed an increase of carbohydrate, protein, SP and WS into 84.761, 3.776, 8.870% and 0.136%, respectively.

  2. Antiinflammatory flavonoids from Artocarpus heterophyllus and Artocarpus communis.

    Science.gov (United States)

    Wei, Bai-Luh; Weng, Jing-Ru; Chiu, Pao-Hui; Hung, Chi-Feng; Wang, Jih-Pyang; Lin, Chun-Nan

    2005-05-18

    The antiinflammatory activities of the isolated flavonoids, including cycloartomunin (1), cyclomorusin (2), dihydrocycloartomunin (3), dihydroisocycloartomunin (4), cudraflavone A (5), cyclocommunin (6), and artomunoxanthone (7), and cycloheterohyllin (8), artonins A (9) and B (10), artocarpanone (11), artocarpanone A (12), and heteroflavanones A (13), B (14), and C (15) from Artocarpus communis and A. heterophyllus, were assessed in vitro by determining their inhibitory effects on the chemical mediators released from mast cells, neutrophils, and macrophages. Compound 4 significantly inhibited the release of beta-glucuronidase and histamine from rat peritoneal mast cells stimulated with P-methoxy-N-methylphenethylamine (compound 48/80). Compound 11 significantly inhibited the release of lysozyme from rat neutrophils stimulated with formyl-Met-Leu-Phe (fMLP). Compounds 8, 10, and 11 significantly inhibited superoxide anion formation in fMLP-stimulated rat neutrophils while compounds 2, 3, 5, and 6 evoked the stimulation of superoxide anion generation. Compound 11 exhibited significant inhibitory effect on NO production and iNOS protein expression in RAW 264.7 cells. The potent inhibitory effect of compound 11 on NO production in lipopolysaccharide (LPS)-activated macrophages, probably through the suppression of iNOS protein expression.

  3. β – Glucanos en almidón nativo de fruta pan y su potencial uso en alimentos funcionales

    Directory of Open Access Journals (Sweden)

    Quezada-Correa, Lady

    2017-09-01

    Full Text Available The main objective of the research was to determine β – glucans content present in native starch from breadfruit (Artocarpus altilis cultivated in Ecuador southern coast and its potential use in functional foods. The starch was obtained throughout the wet method; the sample was subjected to β-glucosidase enzymatic action to determine β-glucan content; glucose content was then quantified through visible UV spectrophotometry techniques at a wavelength of 510 nm. The starch percentage present in the breadfruit cultivar was 55.40%, whereas the β-glucan content was 4.39%. This value reported is higher than those reported previously for some cereals such as oats, barley, rye, wheat, among others, widely used in the food industry. The β-glucans content, present in native starch of breadfruit, makes this cultivar an interesting source of starch to be considered, as a matrix or input, in the manufacturing of nutraceuticals or functional foods.

  4. Physical quality characteristics of the microwave-dried breadfruit powders due to different processing conditions

    Science.gov (United States)

    Taruna, I.; Hakim, A. L.; Sutarsi

    2018-03-01

    Production of breadfruit powder has been an option to make easy its uses in various food processing. Accordingly, there is a need recently to apply advanced drying method, i.e. microwave drying, for improving quality since conventional methods produced highly variable product quality and required longer process. The present work was aimed to study the effect of microwave power and grinding time on physical quality of breadfruit powders. The experiment was done initially by drying breadfruit slices in a microwave dryer at power level of 420, 540, and 720 W and then grinding for 3, 5, and 7 min to get powdery product of less than 80 mesh. The physical quality of breadfruit powders were measured in terms of fineness modulus (FM), average particle size (D), whiteness (WI), total color difference (ΔE), water absorption (Wa), oil absorption (La), bulk density (ρb) and consistency gel (Gc). The results showed that physical quality of powders and its ranged-values included the FM (2.08-2.62), D (0.44-0.68 mm), WI (75.2-77.9), ΔE (7.4-10.5), Wa (5.5-6.2 ml/g), La (0.7-0.9 ml/g), ρb (0.62-0.70 g/cm3) and Gc (41.3-46.8 mm). The experiment revealed that variation of microwave power and grinding time affected significantly the quality of the breadfruit powders. However, microwave power was more dominant factor to affect quality of breadfruit powder in comparison to the grinding time.

  5. Antitumoral and antioxidant activities of lipophilic and phenolic extracts from Cynara cardunculus L. var. altilis (DC)

    OpenAIRE

    Ramos, P.; Guerra, A.; Guerreiro, O.; Santos, S.; Oliveira, H.; Freire, C.; Rocha, S.; Silvestre, A.; Duarte, M. F.

    2014-01-01

    Cynara cardunculus L. var. altilis (DC) (Cca) grows under the semi-arid conditions as those of south of Portugal. Given to high cellulose and hemicelluloses contents, paper pulp production has been proposed [1]. Moreover, the extraction of bioactive compounds could also be considered for an integrated valorization of Cca. Sesquiterpene lactones (SL) and pentacyclic triterpenes, the major constituents of Cca leaves and capitula lipophilic fractions [2] showed lower tryglyceride levels in rat s...

  6. Wound healing properties of Artocarpus heterophyllus Lam.

    Science.gov (United States)

    Gupta, Nilesh; Jain, U K; Pathak, A K

    2009-04-01

    The studies on excision wound healing model reveals significant wound healing activity of the methanolic leaf extract (simple ointment 5%) of "Artocarpus heterophyllus" ham which is comparable with standard (Betadine). In the excision model, the period of epithelization, of the extract treated group was found to be higher than the controlgroup and slightly lesser than standard treated group of animals on the up to 16(th) post wounding day.

  7. Wound healing properties of Artocarpus heterophyllus Lam

    Science.gov (United States)

    Gupta, Nilesh; Jain, U.K.; Pathak, A.K.

    2009-01-01

    The studies on excision wound healing model reveals significant wound healing activity of the methanolic leaf extract (simple ointment 5%) of “Artocarpus heterophyllus” ham which is comparable with standard (Betadine). In the excision model, the period of epithelization, of the extract treated group was found to be higher than the controlgroup and slightly lesser than standard treated group of animals on the up to 16th post wounding day. PMID:22557331

  8. Flavonoids from the roots of Artocarpus heterophyllus.

    Science.gov (United States)

    Yuan, Wen-Jun; Yuan, Jin-Bin; Peng, Jia-Bing; Ding, Yuan-Qing; Zhu, Ji-Xiao; Ren, Gang

    2017-03-01

    Four new flavonoids, artoheteroids A-D (1-4), together with six known ones (5-10), were isolated from the roots of Artocarpus heterophyllus. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR, UV, IR, CD, and HR-ESI-MS. All isolated compounds were screened for their inhibitory abilities against cathepsin K (CatK). Among them, compounds 1-2, 4-6, and 10 were found to have suppression capabilities against CatK with IC 50 values ranging from 1.4 to 93.9μM. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Effect of domestic cooking on the starch digestibility, predicted glycemic indices, polyphenol contents and alpha amylase inhibitory properties of beans (Phaseolis vulgaris) and breadfruit (Treculia africana).

    Science.gov (United States)

    Chinedum, E; Sanni, S; Theressa, N; Ebere, A

    2018-01-01

    The effect of processing on starch digestibility, predicted glycemic indices (pGI), polyphenol contents and alpha amylase inhibitory properties of beans (Phaseolis vulgaris) and breadfruit (Treculia africana) was studied. Total starch ranged from 4.3 to 68.3g/100g, digestible starch ranged from 4.3 to 59.2 to 65.7g/100g for the raw and processed legumes; Resistance starch was not detected in most of the legumes except in fried breadfruit and the starches in both the raw and processed breadfruit were more rapidly digested than those from raw and cooked beans. Raw and processed breadfruit had higher hydrolysis curves than raw and processed beans with the amylolysis level in raw breadfruit close to that of white bread. Raw beans had a low glycemic index (GI); boiled beans and breadfruit had intermediate glycemic indices respectively while raw and fried breadfruit had high glycemic indices. Aqueous extracts of the food samples had weak α-amylase inhibition compared to acarbose. The raw and processed legumes contained considerable amounts of dietary phenols and flavonoids. The significant correlation (r=0.626) between α-amylase inhibitory actions of the legumes versus their total phenolic contents suggests the contribution of the phenolic compounds in these legumes to their α-amylase inhibitory properties. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Artoindonesianin C, a new xanthone derivative from Artocarpus teysmanii.

    Science.gov (United States)

    Makmur, L; Syamsurizal, S; Tukiran, T; Achmad, S A; Aimi, N; Hakim, E H; Kitajima, M; Takayama, H

    2000-02-01

    A new xanthone derivative, artoindonesianin C (1), was isolated from Artocarpus teysmanii, together with two known prenylated flavonoids, cycloartobiloxanthone and artonin J. The structure of artoindonesianin C (1) was determined on the basis of MS and NMR evidence and by comparison with known related compounds.

  11. Hypoglycaemic action of the flavonoid fraction of Artocarpus ...

    African Journals Online (AJOL)

    Hot water extract of mature jak leaves (Artocarpus heterophyllus) is recommended by Ayurvedic and traditional medical practitioners as a treatment for diabetes mellitus. The leaf extract caused the hypoglyceamic effect at a dose of 50 mg/Kg, both in normal and alloxan-diabetic rats. The hypoglycaemic effect was at its ...

  12. BREADFRUIT (Treculia africana MARKETING ACTIVITIES AND RETURNS IN AHIAZU MBAISE LOCAL GOVERNMENT AREA, OF IMO STATE, NIGERIA

    Directory of Open Access Journals (Sweden)

    Ogbonna Christopher EMEROLE

    2013-12-01

    Full Text Available This study on marketing of breadfruits (Treculia africana and returns was done in Ahiazu Mbaise local Government area of Imo State, Nigeria. The specific objectives of the study were to describe socio-economic characteristics of respondents (sellers and buyers of African breadfruit; identify value-chain activities available in processing and its storage in compliance with consumers’ order and preferences; determine factors influencing decision to supply African breadfruit; and constraints with its post-harvest management in the study area. Three-stage random sampling technique was used in selecting locations and respondents through which eighty (80 farm households who gather/harvest, process and sell breadfruits were selected and interviewed with structured questionnaire. Data collected were analyzed using descriptive statistics, and probit regression model. Result revealed that 65.80% of the respondents were females and 81.20% of them were married with mean household size of 9 members. Their literacy level was high as 97.6% of them had at least primary education. Predominant marketing activities were fruit gathering/harvesting, processing, storage and packaging, transportation, and sales. Socio-economic factors of gender, household size, income, level of education, years of farming experience and labour significantly influenced supply of breadfruits to consumers with challenges of seasonal scarcity, and tedious methods of processing deterring the enterprise in the area. We recommended provision of credit support to enable fruit gatherers purchase and use shelling machines and good storage facilities to smooth any fluctuations in supplies during off-seasons and help fight overdependence of households on roots and tubers.

  13. Inhibitory effect of artocarpanone from Artocarpus heterophyllus on melanin biosynthesis.

    Science.gov (United States)

    Arung, Enos Tangke; Shimizu, Kuniyoshi; Kondo, Ryuichiro

    2006-09-01

    In our previous efforts to find new tyrosinase inhibitory materials, we investigated 44 Indonesian medicinal plants belonging to 24 families. Among those plants, the extract of Artocarpus heterophyllus was one of the strongest inhibitors of tyrosinase activity. By activity-guided fractionation of A. heterophyllus wood extract, we isolated artocarpanone, which inhibited both mushroom tyrosinase activity and melanin production in B16 melanoma cells. This compound is a strong candidate as a remedy for hyperpigmentation in human skin.

  14. New phenolic compounds from the twigs of Artocarpus heterophyllus.

    Science.gov (United States)

    Di, X; Wang, S; Wang, B; Liu, Y; Yuan, H; Lou, H; Wang, X

    2013-02-01

    Two new chalcones, artocarpusins A and B (1 and 2), one new flavone, artocarpusin C (3), one new 2-arylbenzofuran derivative, artocarstilene A (4), and 15 flavonoids were isolated from the twigs of Artocarpus heterophyllus. Their structures were established on the basis of extensive spectroscopic analysis. Compounds 9 and 16 showed moderate inhibitory activity on the proliferation of the PC-3 and H460 cell lines.

  15. Prenylated flavone from roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer and its biological activities

    International Nuclear Information System (INIS)

    Panthong, Kanda; Tohdee, Kanogwan; Hutadilok-Towatana, Nongporn; Voravuthikunchai, Supayang P.; Chusri, Sasitorn

    2013-01-01

    One new prenylated flavone, 2,8-dihydroxy-3,10-dimethoxy-6-(2-methyl-1-propen-1-yl)- 6H,7H-[1]benzopyrano[4,3-b][1]-benzopyran-7-one, together with 24 known compounds were isolated from crude acetone extract from the roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer. Their structures were determined by 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data. The antioxidant and antibacterial activities of the isolated compounds were evaluated. The new compound showed potent antioxidant activity against DPPH• and superoxide with IC 50 values of 0.033 and 0.125 mg mL -1 , respectively. Significant antibacterial activity against Acinetobacter baumannii was observed with MIC value of 50 μg mL -1 . (author)

  16. Prenylated flavone from roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer and its biological activities

    Energy Technology Data Exchange (ETDEWEB)

    Panthong, Kanda [Prince of Songkla University (Thailand). Natural Product Research Center of Excellence; Tohdee, Kanogwan [Prince of Songkla University (Thailand). Faculty of Science. Department of Chemistry; Hutadilok-Towatana, Nongporn [Prince of Songkla University (Thailand). Dept. of Biochemistry; Voravuthikunchai, Supayang P. [Prince of Songkla University (Thailand). Faculty of Science. Department of Microbiology; Chusri, Sasitorn [Prince of Songkla University (Thailand). Faculty of Traditional Thai Medicine

    2013-10-15

    One new prenylated flavone, 2,8-dihydroxy-3,10-dimethoxy-6-(2-methyl-1-propen-1-yl)- 6H,7H-[1]benzopyrano[4,3-b][1]-benzopyran-7-one, together with 24 known compounds were isolated from crude acetone extract from the roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer. Their structures were determined by 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data. The antioxidant and antibacterial activities of the isolated compounds were evaluated. The new compound showed potent antioxidant activity against DPPH Bullet and superoxide with IC{sub 50} values of 0.033 and 0.125 mg mL{sup -1}, respectively. Significant antibacterial activity against Acinetobacter baumannii was observed with MIC value of 50 {mu}g mL{sup -1}. (author)

  17. Antiproliferative Effects of Cynara cardunculus L. var. altilis (DC Lipophilic Extracts

    Directory of Open Access Journals (Sweden)

    Patrícia A. B. Ramos

    2016-12-01

    Full Text Available Besides being traditionally used to relieve hepatobiliary disorders, Cynara cardunculus L. has evidenced anticancer potential on triple-negative breast cancer (TNBC. This study highlights the antiproliferative effects of lipophilic extracts from C. cardunculus L. var. altilis (DC leaves and florets, and of their major compounds, namely cynaropicrin and taraxasteryl acetate, against MDA-MB-231 cells. Our results demonstrated that MDA-MB-231 cells were much less resistant to leaves extract (IC50 10.39 µg/mL than to florets extract (IC50 315.22 µg/mL, during 48 h. Moreover, leaves extract and cynaropicrin (IC50 6.19 µg/mL suppressed MDA-MB-231 cells colonies formation, via an anchorage-independent growth assay. Leaves extract and cynaropicrin were also assessed regarding their regulation on caspase-3 activity, by using a spectrophotometric assay, and expression levels of G2/mitosis checkpoint and Akt signaling pathway proteins, by Western blotting. Leaves extract increased caspase-3 activity, while cynaropicrin did not affect it. Additionally, they caused p21Waf1/Cip1 upregulation, as well as cyclin B1 and phospho(Tyr15-CDK1 accumulation, which may be related to G2 cell cycle arrest. They also downregulated phospho(Ser473-Akt, without changing total Akt1 level. Cynaropicrin probably contributed to leaves extract antiproliferative action. These promising insights suggest that cultivated cardoon leaves lipophilic extract and cynaropicrin may be considered toward a natural-based therapeutic approach on TNBC.

  18. Tyrosinase inhibitors from the wood of Artocarpus heterophyllus.

    Science.gov (United States)

    Nguyen, Nhan Trung; Nguyen, Mai Ha Khoa; Nguyen, Hai Xuan; Bui, Ngan Kim Nguyen; Nguyen, Mai Thanh Thi

    2012-11-26

    From the methanolic-soluble extract of the wood of Artocarpus heterophyllus, four new flavones, artocarmins A-D (1-4), and three new chalcones, artocarmitins A-C (5-7), have been isolated together with 13 known compounds. Their structures were determined on the basis of the spectroscopic data. Compounds 1-4, 6, 7, 9-16, and 20 displayed significant tyrosinase inhibitory activity. The most active compound, morachalcone A (12) (IC50, 0.013 μM), was 3000 times more active as a tyrosinase inhibitor than a positive control, kojic acid (IC50, 44.6 μM).

  19. Ameliorative Activity of Ethanolic Extract of Artocarpus heterophyllus Stem Bark on Alloxan-induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Basiru Olaitan Ajiboye

    2018-03-01

    Full Text Available Purpose: Diabetes mellitus is one of the major endocrine disorders, characterized by impaired insulin action and deficiency. Traditionally, Artocarpus heterophyllus stem bark has been reputably used in the management of diabetes mellitus and its complications. The present study evaluates the ameliorative activity of ethanol extract of Artocarpus heterophyllus stem bark in alloxan-induced diabetic rats. Methods: Diabetes mellitus was induced by single intraperitoneal injection of 150 mg/kg body weight of alloxan and the animals were orally administered with 50, 100 and 150 mg/kg body weight ethanol extract of Artocarpus heterophyllus stem bark once daily for 21 days. Results: At the end of the intervention, diabetic control rats showed significant (p0.05 different with non-diabetic rats. Conclusion: The results suggest that ethanol extract of Artocarpus heterophyllus stem bark may be useful in ameliorating complications associated with diabetes mellitus patients.

  20. Isotherm Studies of Equilibrium Sorption of Cu2+ and Cd2+ from Aqueous Solutions by Modified and Unmodified Breadfruit Seed Hull

    Directory of Open Access Journals (Sweden)

    Christopher Uchechukwu Sonde

    2015-09-01

    Full Text Available The ability of an economically cheap adsorbent material of natural origin, African breadfruit seed hull, was assessed for Cu(II and Cd(II ions’ adsorption from aqueous solutions. The effects of adsorbent dose, particle size and initial metal ion concentrations were investigated in a batch adsorption process. The experimental data were analyzed using five two-parameter isotherm equations (i.e., Langmuir, Freundlich, Temkin, Harkins-Jura and Halsey isotherm models. Freundlich and Halsey models provided the best description for the adsorption data while the other three models gave fairly good interpretation to the experimental adsorption data. The maximum adsorption capacity corresponding to saturation of sites (qmax, obtained from the Langmuir plots, were 7.76 and 8.06 mg g-1 for Cu(II and Cd(II onto the unmodified breadfruit seed hull (UBSH and 12.67 and 13.97 mg g-1, respectively for Cu(II and Cd(II adsorption onto the modified breadfruit seed hull (MBSH. The experimental results showed that there was an enhancement in the removal of the metal ions by the mercaptoacetic acid-modified breadfruit seed hull. DOI: http://dx.doi.org/10.17807/orbital.v7i3.625 

  1. Ameliorative Activity of Ethanolic Extract of Artocarpus heterophyllus Stem Bark on Alloxan-induced Diabetic Rats.

    Science.gov (United States)

    Ajiboye, Basiru Olaitan; Adeleke Ojo, Oluwafemi; Adeyonu, Oluwatosin; Imiere, Oluwatosin; Emmanuel Oyinloye, Babatunji; Ogunmodede, Oluwafemi

    2018-03-01

    Purpose: Diabetes mellitus is one of the major endocrine disorders, characterized by impaired insulin action and deficiency. Traditionally, Artocarpus heterophyllus stem bark has been reputably used in the management of diabetes mellitus and its complications. The present study evaluates the ameliorative activity of ethanol extract of Artocarpus heterophyllus stem bark in alloxan-induced diabetic rats. Methods: Diabetes mellitus was induced by single intraperitoneal injection of 150 mg/kg body weight of alloxan and the animals were orally administered with 50, 100 and 150 mg/kg body weight ethanol extract of Artocarpus heterophyllus stem bark once daily for 21 days. Results: At the end of the intervention, diabetic control rats showed significant (pArtocarpus heterophyllus stem bark most especially at 150 mg/kg body weight which exhibited no significant (p>0.05) different with non-diabetic rats. Conclusion: The results suggest that ethanol extract of Artocarpus heterophyllus stem bark may be useful in ameliorating complications associated with diabetes mellitus patients.

  2. SCHISTOSOMICIDAL ACTIVITY OF THE CRUDE EXTRACT OF ARTOCARPUS LAKOOCHA.

    Science.gov (United States)

    Preyavichyapugdee, Narin; Sangfuang, Manaw; Chaiyapum, Saowapark; Sriburin, Suwatcharaporn; Pootaeng-on, Yupa; Chusongsang, Phiraphol; Jiraungkoorskul, Wannee; Preyavichyapugdee, Mananya; Sobhon, Prasert

    2016-01-01

    Puag-Haad is a traditional anthelmintic drug used to treat taeniasis in Thailand and Lao PDR. It is derived from the aqueous extract of the plant Artocarpus lakoocha. We investigated the in vitro anthelmintic properties of Puag-Haad against Schistosoma mansoni. Adult worms were incubated in M-199 medium containing 250, 500 and 750 μg/ml of Puag-Haad or praziquantel (PZQ) at a concentration of 175 μg/ml for 3, 6, 12 and 24 hours. The relative motility (RM value), survival index (SI) and tegument alterations seen under scanning electron microscope were assessed at each incubation time. The results showed the crude extract of A. lakoocha at a concentration of 250 μg/ml was more effective in causing damage than PZQ at a concentration of 175 μg/ml using RM and SI values. The major target organ affected by Puag-Haad was the tegument. The damage was greater at higher concentrations of the crude extract. It is likely tetrahydroxystilbene (THS), the main compound in Puag-Haad, caused the damage. THS could be a future candidate as a schistosomal drug. Further studies are needed to explore its mechanism, efficiency and safety in vivo.

  3. Isolation and Purification of Jacalin from Artocarpus Heterophyllus Lam

    Directory of Open Access Journals (Sweden)

    M.R. Othman

    2010-09-01

    Full Text Available This paper presents investigation results of saturation conditions needed for purification of jacalin lectin from the extract seeds of Artocarpus heterophyllus by ammonium precipitation and affinity chromatography on Galactose-Affi gel Hz. Three different aspects of parameters encompassing the percentage of saturation of ammonium sulfate precipitation, the presence of ammonium sulfate on Lowry method and the suitable galactose concentration for optimum elution of the protein from Galactose-Affi gel Hz were investigated. With three different sets of fractional saturation of jacalin purification using ammonium sulfate precipitation, the maximum yield of 0.463 g/g was achieved at 0-90% saturation range in the absence of dialysis. Maximum yield of 0.425 g/g was obtained at 30-60% and 0-90% saturation range in the presence of dialysis. The result from this work also indicates that excessive quantity of NH4SO4 interferes with Lowry method for protein determination substantially. The 0-90% saturation range was found to be more potentially appropriate for large scale application than 30-60% saturation, since the former involves only 1 step NH4SO4 addition. From the affinity chromatography, elution of 0.2 M galactose (in 0.15 M NaCl from Galactose-Affi gel Hz produced the maximum peak profile and jacalin concentration. A reduction or increase in galactose concentration of more than 0.2 M did not increase concentration of purified jacalin purified using this method.

  4. Scavenger and antioxidant properties of prenylflavones isolated from Artocarpus heterophyllus.

    Science.gov (United States)

    Ko, F N; Cheng, Z J; Lin, C N; Teng, C M

    1998-07-15

    The antioxidant properties of prenylflavones, isolated from Artocarpus heterophyllus Lam., was evaluated in this study. Among them, artocarpine, artocarpetin, artocarpetin A, and cycloheterophyllin diacetate and peracetate had no effect on iron-induced lipid peroxidation in rat brain homogenate. They also did not scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl. In contrast, cycloheterophyllin and artonins A and B inhibited iron-induced lipid peroxidation in rat brain homogenate and scavenged 1,1-diphenyl-2-picrylhydrazyl. They also scavenged peroxyl radicals and hydroxyl radicals that were generated by 2,2'-azobis(2-amidinopropane) dihydrochloride and the Fe3+-ascorbate-EDTA-H2O2 system, respectively. However, they did not inhibit xanthine oxidase activity or scavenge superoxide anion, hydrogen peroxide, carbon radical, or peroxyl radicals derived from 2,2'-azobis(2,4-dimethylvaleronitrile) in hexane. Moreover, cycloheterophyllin and artonins A and B inhibited copper-catalyzed oxidation of human low-density lipoprotein, as measured by fluorescence intensity, thiobarbituric acid-reactive substance and conjugated-diene formations and electrophoretic mobility. It is concluded that cycloheterophyllin and artonins A and B serve as powerful antioxidants against lipid peroxidation when biomembranes are exposed to oxygen radicals.

  5. Efecto de la urea y del tiempo en la fermentación en estado sólido de la harina de frutos del árbol del pan (Artocarpus altilis

    Directory of Open Access Journals (Sweden)

    Odelin Brea-Maure

    2015-07-01

    Full Text Available Para evaluar el efecto de la inclusión de niveles de urea y del tiempo sobre los indicadores fermentativos y químicos de la harina de frutos del árbol del pan, fermentada en estado sólido, se mezcló homogéneamente con esta, sulfato de magnesio (0,2%, premezcla mineral (0,5% y agua destilada. Se utilizó un diseño completamente aleatorizado con arreglo factorial (4x4, con 4 repeticiones por tratamiento para evaluar el efecto de la urea (0; 0,5; 1,0 y 1,5% y cuatro tiempos de fermentación (0, 12, 24 y 48 h. En el estudio, a medida que se incrementa el nivel de urea, aumenta la concentración de amoníaco y, por ende, el pH, lo que depende, en menor o mayor grado, de la concentración de los ácidos orgánicos (AGCC y láctico. Al final del proceso fermentativo hubo una disminución en la MS (P<0,001 a medida que transcurrió el tiempo de fermentación. La adición de 1,5% de urea produjo un incremento en la concentración de PB a las 24 y 48 h sin diferencia entre ellos; algo similar ocurrió con la PV y la relación entre ellos (PV:PB*100 sin interacción entre los factores en estudios. A las 48 h hubo un aumento mayor de 2,15 unidades porcentuales en la PB con respecto a la hora cero. Se concluye que la adición de urea en el proceso de fermentación de la harina de frutos del árbol del pan en estado sólido incrementa la síntesis de proteína microbiana.

  6. Artocarpus hirsutus Lam. of Moraceae is a large evergreen tree with ...

    Indian Academy of Sciences (India)

    Artocarpus hirsutus Lam. of Moraceae is a large evergreen tree with milky latex. This species occurs wild and is also cultivated for its fruit, which is edible. Leaves are simple and dark green. The branchlets are covered with rust-brown hairs. Inflorescence is axillary. The female inflorescence is globose with individualjlowers ...

  7. Nutritional assessment of a jackfruit (Artocarpus heterophyllus) meal.

    Science.gov (United States)

    Hettiaratchi, U P K; Ekanayake, S; Welihinda, J

    2011-06-01

    The mature jackfruit (Artocarpus heterophyllus) is consumed in Sri Lanka either as a main meal or a meal accompaniment. However, there is no scientific data on the nutrient compositions of cooked jackfruit meals. Thus, the objective of the study was to carry out a nutritional assessment of a composite jackfruit breakfast meal comprising seeds and flesh. A jackfruit meal comprising of flesh (80% available carbohydrate) and seeds (20% available carbohydrate) was included in the study. The study was carried out in a random cross over design. Setting University of Sri Jayewardenepura. Study participants Healthy individuals (n=10, age: 20-30 yrs). The macronutrient contents, rapidly and slowly available glucose (SAG) contents, water solubility index of the jackfruit meal were determined according to standard methods. The GI of the meal was calculated according to FAO/WHO guidelines. The moisture content of the boiled jackfruit flesh was high (82% FW). Jack seeds contained 4.7% protein (FW), 11.1% total dietary fibre (FW) and 8% resistant starch (FW). Jackfruit meal elicited a GI of 75. The Glycaemic Load (GL) of the normal serving size of the meal is medium. The slowly available glucose (SAG) percentage of jackfruit meal (30%) was twice that of the standard. The boiled jackfruit flesh contained disintegrated starch granules while seeds contained intact swollen and disintegrated granules. The jackfruit seeds are a good source of starch (22%) and dietary fibre. The meal is categorized as a low GI meal. The low GI could be dueto the collective contributions from dietary fibre, slowly available glucose and un-gelatinised (intact) starch granules in the seeds.

  8. Characterization of antiproliferative activity constituents from Artocarpus heterophyllus.

    Science.gov (United States)

    Zheng, Zong-Ping; Xu, Yang; Qin, Chuan; Zhang, Shuang; Gu, Xiaohong; Lin, Yingying; Xie, Guobin; Wang, Mingfu; Chen, Jie

    2014-06-18

    Artocarpus heterophyllus is an evergreen fruit tree cultivated in many tropical regions. Previous studies have shown that some of its compositions exhibited potential tyrosinase inhibition activities. This study indentified 8 new phenolic compounds, artoheterophyllins E-J (1-6), 4-geranyl-2',3,4',5-tetrahydroxy-cis-stilbene (7), and 5-methoxymorican M (8) and 2 new natural compounds (9 and 10), 2,3-dihydro-5,7-dihydroxy-2-(2-hydroxy-4-methoxyphenyl)-4H-benzopyran-4-one and 6-[(1S,2S)-1,2-dihydroxy-3-methylbutyl]-2-(2,4-dihydroxyphenyl)-5-hydroxy-7-methoxy-3-(3-methyl-2-buten-1-yl)-4H-1-benzopyran-4-one, together with 23 known compounds (11-33), from the ethanol extract of the wood of A. heterophyllus. The structures of the eight new compounds (1-8) and two new natural compounds were established by extensive 1D- and 2D-NMR experiments. The anticancer effects of the isolated compounds were examined in MCF-7, H460, and SMMC-7721 human cancer cell lines by MTT assay. Compounds 5, 11, 12, and 30 significantly reduced the cell viabilities of these cell lines. Especially, compounds 11 and 30 resulted in more potent cytotoxicity than the positive control, 5-fluorouracil (5-Fu), in SMMC-7721 cell line, with IC50 values of 15.85 and 12.06 μM, whereas compound 30 exhibited more potent cytotoxicity than 5-Fu in NCI-H460 cell line, with an IC50 value of 5.19 μM. In addition, this study suggests that compounds 11 and 30 from the wood of A. heterophyllus have anticancer potential via MAPK pathways.

  9. Extraction and characterization of artocarpus integer gum as pharmaceutical excipient.

    Science.gov (United States)

    Farooq, Uzma; Malviya, Rishabha; Sharma, Pramod Kumar

    2014-01-01

    Natural polymers are widely used as excipients in pharmaceutical formulations. They are easily available, cheap and less toxic as compared to synthetic polymers. This study involves the extraction and characterization of kathal (Artocarpus integer) gum as a pharmaceutical excipient. Water was used as a solvent for extraction of the natural polymer. Yield was calculated with an aim to evaluate the efficacy of the process. The product was screened for the presence of Micrometric properties, and swelling index, flow behavior, surface tension, and viscosity of natural polymers were calculated. Using a water based extraction method, the yield of gum was found to be 2.85%. Various parameters such as flow behavior, organoleptic properties, surface tension, viscosity, loss on drying, ash value and swelling index together with microscopic studies of particles were done to characterize the extracted gum. The result showed that extracted kathal gum exhibited excellent flow properties. The gum was investigated for purity by carrying out chemical tests for different phytochemical constituents and only carbohydrates were found to be present. It had a good swelling index (13 ± 1). The pH and surface tension of the 1% gum solution were found to be 6 ± 0.5 and 0.0627 J/m2, respectively. The ash values such as total ash, acid insoluble ash, and water soluble ash were found to be 18.9%, 0.67% and 4% respectively. Loss on drying was 6.61%. The extracted gum was soluble in warm water and insoluble in organic solvents. The scanning electron micrograph (SEM) revealed rough and irregular particles of the isolated polymer. The results of the evaluated properties showed that kathal-derived gum has acceptable pH and organoleptic properties and can be used as a pharmaceutical excipient to formulate solid oral dosage forms.

  10. Mitogenic activity of new lectins from seeds of wild Artocarpus species from Vietnam.

    Science.gov (United States)

    Blasco, E; Ngoc, L D; Aucouturier, P; Preud'Homme, J L; Barra, A

    1996-05-01

    Proliferative response of human peripheral blood mononuclear cells (PBMC) stimulated by new lectins purified from seeds of differents Artocarpus species from Vietnam (A. asperulus, A. heterophyllus, A. masticata, A. melinoxylus, A. parva and A. petelotii) was studied and compared to those of the lectin jacalin purified from jackfruit (A. heterophyllus) seeds collected in the island La Réunion. All lectins stimulated human PBMC to proliferate, with a variable efficiency of the mitogenic activity. Phenotypic analysis of cells recovered after 7 day-cultures showed that these lectins mostly stimulated CD4+ T lymphocytes. These results suggest that these lectins from different Artocarpus species are similar in terms of their mitogenic activity although their structural features are not identical.

  11. Penggunaan ekstrak kayu nangka (artocarpus heterophyllus sebagai bahan pewarna untuk kulit

    Directory of Open Access Journals (Sweden)

    Emiliana Kasmudjiastuti

    2001-12-01

    Full Text Available Abstract The objective of this experiment was to evaluate the effect Alum content and time of mordanting on the rub fastness. Natural dyes from wood dyes Artocarpus heterophyllus haved an intermediate acid resistance (acetic acid, a good alkali resistance (natrium carbonat0 but they had very low sulphur acid resistance and low formic acid resistence. Natural dyes from wood dyes Artocarpus heterophyllus could be used as colouring matter on goat skin distributed throughout the substance of the fibre goat skin. Their rub fastness was a good (dry and wet. The variation of amounts alum (1%, 2%, 3% and treatment of mordanting (before and during dyeing did not influence on rub fastness (dry and wet.

  12. One-step synthesis of highly-biocompatible spherical gold nanoparticles using Artocarpus heterophyllus Lam. (jackfruit fruit extract and its effect on pathogens

    Directory of Open Access Journals (Sweden)

    Nagaraj Basavegowda

    2015-02-01

    It can be concluded that the fruit of Artocarpus heterophyllus Lam. can be good source for synthesis of gold nanoparticles which showed antimicrobial activity against investigated microbes, in particul E. coli, and Streptobacillus. An important outcome of this study will be the development of value-added products from the medicinal plant Artocarpus heterophyllus Lam. for the biomedical and nanotechnology-based industries.

  13. Inhibitory effect of isoprenoid-substituted flavonoids isolated from Artocarpus heterophyllus on melanin biosynthesis.

    Science.gov (United States)

    Arung, Enos Tangke; Shimizu, Kuniyoshi; Kondo, Ryuichiro

    2006-07-01

    Isoprenoid-substituted flavonoids were isolated from the wood of Artocarpus heterophyllus by means of activity-guided fractionation. Artocarpin (1), cudraflavone C (2), 6-prenylapigenin (3), kuwanon C (4), norartocarpin (5) and albanin A (6) inhibited melanin biosynthesis in B16 melanoma cells without inhibiting tyrosinase. A structure-activity investigation indicated that the presence of the isoprenoid-substituted moiety enhanced the inhibitory activity on melanin production in B16 melanoma cells.

  14. Kajian Karakteristik Fisikokimia Dan Sensoris Fruit Leather Nangka (Artocarpus Heterophyllus) Dengan Penambahan Karaginan

    OpenAIRE

    Fitantri, Arinda Laksmi; Parnanto, Nur Her Riyadi; Praseptiangga, Danar

    2014-01-01

    Dalam penelitian ini dibuat fruit leather nangka (Artocarpus heterophyllus) dengan variasi penambahan karaginan (0%; 0,3%; 0,6% dan 0,9%) dengan tujuan untuk mengetahui karakteristik fisikokimia (kuat tarik, kadar air, kadar abu, Aw, kadar serat pangan, dan kadar gula reduksi) serta karakteristik sensoris fruit leather nangka. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu konsentrasi karaginan. Masing-masing perlakuan dilakukan dua kali ulangan sam...

  15. A sperm-agglutinating lectin from seeds of Jack fruit (Artocarpus heterophyllus).

    Science.gov (United States)

    Namjuntra, P; Muanwongyathi, P; Chulavatnatol, M

    1985-04-30

    A lectin specific for N-acetylgalactosamine was isolated from seed extract of Jack fruit (Artocarpus heterophyllus) by ammonium sulfate precipitation, followed by affinity chromatography on a Affigel-galactosamine-agarose column. The lectin possessed agglutinating activities for human and rat sperm as well as human red blood cells. It was found to have Mr = 62,000 consisting of two dissimilar subunits of Mr = 18,000 and 13,000. It also cross-reacted with an antibody against the lectin of Osage Orange (Maclura pomifera).

  16. Evaluation of antioxidant capacity and phenol content in jackfruit (Artocarpus heterophyllus Lam.) fruit pulp.

    Science.gov (United States)

    Jagtap, Umesh B; Panaskar, Shrimant N; Bapat, V A

    2010-06-01

    The antioxidant capacity of jackfruit (Artocarpus heterophyllus Lam. Fam. Moracae) fruit pulp (JFP) obtained from Western Ghats India was determined by evaluating the scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing power assays and N, N-dimethyl-p-phenylendiamine (DMPD) radical cation decolorization assay. JFP was analyzed for total phenolic content (TPC) and total flavonoids content (TFC). The ethanol and water are the best solvents for the extracting phenols and flavonoids from the JFP. The antioxidant activities of JFP extracts were correlated with the total phenolic and flavonoids content. The results indicated that the jackfruit pulp is one natural source of antioxidant compounds.

  17. Effect of Essential Oil of Attarasa Leaves (Litsea Cubeba Lour. Pers) on Physico-Mechanical and Microstructural Properties Of Breadfruit Starch-Alginate Edible Film

    International Nuclear Information System (INIS)

    Zuhra, C.F.; Kaban, J.; Marpongahtun; Erman Munir

    2013-01-01

    Research on preparation of edible film from breadfruit starch and alginate incorporated with essential oil of attarasa leaves (Litsea cubeba Lour Pers.) has been done. The film was evaluated of their thickness, tensile strength, elongation at break, water vapor transmission rate (WVTR) and microstructural properties by Scanning Electron Microscopy (SEM). Incorporation of the oil increased thickness of film from 0.033 mm to 0.036 mm, tensile strength from 32.8 to 37.0 MPa, elongation at break from 48.92 % to 50.43 % but water vapor transmission rate (WVTR) decreased from 142.9 g/ m 2 .hour to 120.3 g/ m 2 .hour. However SEM analysis showed that the surface microstructural of the film was more rough and solid compare with film without incorporation of essential oil. (author)

  18. A flower in fruit's clothing: Pollination of jackfruit Artocarpus heterophyllus, Moraceae) by a new species of gall midge, Clinodiplosis ultracrepidata sp. nov. (Diptera: Cecidomyiidae)

    Science.gov (United States)

    Premise of the Research: Jackfruit (Artocarpus heterophyllus, Moraceae) is an emerging but underutilized crop whose pollination is poorly understood. We present a multidimensional investigation of the reproductive biology and chemical ecology of jackfruit and a putative pollinator, Clinodiplosis ult...

  19. Identifikasi Keragaman Genetik Dengan Karakter Morfologi Artocarpus heterophyllus Lamk Nangka Kalimantan Barat, Indonesia

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    Nugraha Banu Safitri

    2018-01-01

    Full Text Available Nangka Artocarpus heterophyllus Lamk. Merupakan buah yang sudah lama dikenal namun belum banyak penelitian tentang nangka.  Kalimantan  Barat  merupakan  provinsi yang kaya plasma  nutfah baik  tanaman  hutan,  perkebunan dan tanaman pertanian. Penelitian ini bertujuan  untuk  mengetahui  keragaman  genetik dengan karakter morfologi dan untuk mengetahui  nilai  similaritas jarak antar aksesi  keragaman nangka di Kalimantan Barat.  Metode yang digunakan  adalah metode observasi pengambilan sampel menggunakan  purposive sampling. Identifikasi morfologi menggunakan data kuantitatif dan data kualitatif dengan passport data IPGRI diubah menjadi data numerik. Hasil identifikasi keragaman morfologi pada nangka diperoleh tiga kelompok besar dalam skala 20 analisis cluster menggunakan software SPSS versi 22. Hasil pengelompokan pertama Kubu Raya02 dan Pontianak01; kelompok kedua Sambas01,Singkawang02, Sambas02, Kubu Raya05, Kubu Raya01 dan Singkawang01; kelompok ketiga terdiri Pontianak02, Kuburaya04 dan  Kubu Raya03. Hasil analisis similaritas menunjukkan bahwa memiliki nilai koefisien similaritas tertinggi pasangan Kubu Raya02 dengan Kubu Raya02 dan nilai koefisien terendah pada pasangan Kubu Raya04 dengan Pontianak02. Dengan identifikasi kelompok dapat merancang dan merencanakan pemuliaan tanaman dengan metode persilangan konfensional maupun menggunakan rekayasa genetika. Pemetaan menggunakan analisis cluster memberikan peran penting untuk memperbaiki sifat-sifat tanaman yang dikehendaki.Kata kunci: Artocarpus heterophyllus. Lamk, Genetik, Identifikasi, Kalimantan Barat, Keragaman, Morfologi, Nangka, Pengelompokan.

  20. Anti-inflammatory effects of phenolic compounds isolated from the fruits of Artocarpus heterophyllus.

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    Fang, Song-Chwan; Hsu, Chin-Lin; Yen, Gow-Chin

    2008-06-25

    Artocarpus heterophyllus Lam is a large evergreen tree cultivated throughout Southeast Asia for its fruits. Its leaves and roots have been used for medicinal purposes. The aim of this work was to study the in vitro anti-inflammatory effects of phenolic compounds isolated from the ethyl acetate extracts of the fruits of Artocarpus heterophyllus. Three phenolic compounds were characterized as artocarpesin [5,7,2',4'-tetrahydroxy-6-(3-methylbut-3-enyl) flavone] ( 1), norartocarpetin (5,7,2',4'-tetrahydroxyflavone) ( 2), and oxyresveratrol [ trans-2,4,3',5'-tetrahydroxystilbene] ( 3) by spectroscopic methods and through comparison with data reported in the literatures. The anti-inflammatory effects of the isolated compounds ( 1- 3) were evaluated by determining their inhibitory effects on the production of proinflammatory mediators in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophage cells. These three compounds exhibited potent anti-inflammatory activity. The results indicated that artocarpesin ( 1) suppressed the LPS-induced production of nitric oxide (NO) and prostaglandin E 2 (PGE 2) through the down-regulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) protein expressions. Thus, artocarpesin ( 1) may provide a potential therapeutic approach for inflammation-associated disorders.

  1. Thermodynamic properties of water sorption of jackfruit (Artocarpus heterophyllus Lam. as a function of moisture content

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    Ana Paula Prette

    2013-03-01

    Full Text Available The Jackfruit tree is one of the most significant trees in tropical home gardens and perhaps the most widespread and useful tree in the important genus Artocarpus. The fruit is susceptible to mechanical and biological damage in the mature state, and some people find the aroma of the fruit objectionable, particularly in confined spaces. The dehydration process could be an alternative for the exploitation of this product, and the relationship between moisture content and water activity provides useful information for its processing and storage. The aim of this study was to determine the thermodynamic properties of the water sorption of jackfruit (Artocarpus heterophyllus Lam. as a function of moisture content. Desorption isotherms of the different parts of the jackfruit (pulp, peduncle, mesocarp, peel, and seed were determined at four different temperatures (313.15, 323.15, 333.15, and 343.15 K in a water activity range of 0.02-0.753 using the static gravimetric method. Theoretical and empirical models were used to model the desorption isotherms. An analytical solution of the Clausius-Clapeyron equation was proposed to calculate the isosteric heat of sorption, the differential entropy, and Gibbs' free energy using the Guggenhein-Anderson-de Boer and Oswin models considering the effect of temperature on the hygroscopic equilibrium.

  2. Origin and diversity of an underutilized fruit tree crop, cempedak (Artocarpus integer, Moraceae).

    Science.gov (United States)

    Wang, Maria M H; Gardner, Elliot M; Chung, Richard C K; Chew, Ming Yee; Milan, Abd Rahman; Pereira, Joan T; Zerega, Nyree J C

    2018-06-06

    Underutilized crops and their wild relatives are important resources for crop improvement and food security. Cempedak [Artocarpus integer (Thunb). Merr.] is a significant crop in Malaysia but underutilized elsewhere. Here we performed molecular characterization of cempedak and its putative wild relative bangkong (Artocarpus integer (Thunb). Merr. var. silvestris Corner) to address questions regarding the origin and diversity of cempedak. Using data from 12 microsatellite loci, we assessed the genetic diversity and genetic/geographic structure for 353 cempedak and 175 bangkong accessions from Malaysia and neighboring countries and employed clonal analysis to characterize cempedak cultivars. We conducted haplotype network analyses on the trnH-psbA region in a subset of these samples. We also analyzed key vegetative characters that reportedly differentiate cempedak and bangkong. We show that cempedak and bangkong are sister taxa and distinct genetically and morphologically, but the directionality of domestication origin is unclear. Genetic diversity was generally higher in bangkong than in cempedak. We found a distinct genetic cluster for cempedak from Borneo as compared to cempedak from Peninsular Malaysia. Finally, cempedak cultivars with the same names did not always share the same genetic fingerprint. Cempedak origins are complex, with likely admixture and hybridization with bangkong, warranting further investigation. We provide a baseline of genetic diversity of cempedak and bangkong in Malaysia and found that germplasm collections in Malaysia represent diverse coverage of the four cempedak genetic clusters detected. © 2018 Botanical Society of America.

  3. Effect of Artocarpus heterophyllus and Asteracanthus longifolia on glucose tolerance in normal human subjects and in maturity-onset diabetic patients.

    Science.gov (United States)

    Fernando, M R; Wickramasinghe, N; Thabrew, M I; Ariyananda, P L; Karunanayake, E H

    1991-03-01

    Investigations were carried out to evaluate the effects of hot-water extracts of Artocarpus heterophyllus leaves and Asteracanthus longifolia whole plant material on the glucose tolerance of normal human subjects and maturity-onset diabetic patients. The extracts of both Artocarpus heterophyllus and Asteracanthus longifolia significantly improved glucose tolerance in the normal subjects and the diabetic patients when investigated at oral doses equivalent to 20 g/kg of starting material.

  4. Qualidade de jaca (Artocarpus heterophyllus, Lam. desidratada sob diferentes condições de processo Quality of jackfruit (Artocarpus heterophyllus, Lam. dehydrated under different processing conditions

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    Lenice Freiman de Oliveira

    2011-09-01

    Full Text Available Os frutos da jaqueira (Artocarpus heterophyllus Lam. são consumidos na forma in natura pelas mais diversas camadas da população e sua alta perecibilidade leva a um índice elevado de perda pós-colheita, acarretando prejuízos para os produtores dessa frutífera. Sendo assim, o objetivo desse trabalho foi avaliar o efeito da temperatura de secagem (50, 60 e 70 °C e do teor de umidade final (20 e 25% sobre as características químicas e sensoriais da jaca desidratada. Foram realizadas análises de matéria seca, cinzas, proteínas, lipídeos, carboidratos, além da avaliação sensorial. Os resultados globais obtidos mostraram que a jaca desidratada possui teor de matéria seca de 74,2 a 80,9%, cinzas de 3,1 a 3,8%, proteínas de 3,2 a 6,6%, lipídeos de 0,09 a 1,2% e carboidratos de 89,8 a 92,4%. A avaliação sensorial mostrou que o produto de maior aceitação (média de 5,95 foi aquele desidratado a 50 °C e com 20% de umidade final, demonstrando que este pode ser uma alternativa alimentar para as regiões produtoras de jaca.Jackfruits (Artocarpus heterophyllus Lam. are consumed in natura by the most diverse sectors of the population, and their high perishability lead to high indexes of postharvest losses, leading to prejudice for the producers of this fruit. Thus the objectives of this work were to evaluate the effects of the drying temperature (50, 60 and 70 °C and the final moisture content (20 and 25% on the chemical and sensory characteristics of dried jackfruit. The overall results obtained showed that the dehydrated jackfruit had a dry matter content of from 74.2 to 80.9%, ash content from 3.1 to 3.8%, protein content from 3.2 to 6.6%, fat content from 0.09 to 1.2% and carbohydrate content from 89.8 to 92.4%. The sensory evaluation showed that the most accepted product (average of 5.95 was dehydrated at 50 °C, with a final moisture content of 20%, showing that this could be a food alternative for areas producing jackfruit.

  5. Development and characterization of EST-SSR markers for Artocarpus hypargyreus (Moraceae).

    Science.gov (United States)

    Liu, Haijun; Tan, Weizheng; Sun, Hongbin; Liu, Yu; Meng, Kaikai; Liao, Wenbo

    2016-12-01

    Polymorphic microsatellite markers were developed for Artocarpus hypargyreus (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species. Based on the transcriptome data of A. hypargyreus , 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displayed clear polymorphisms across the 67 individuals from four populations of A. hypargyreus . The results showed the number of alleles per locus ranged from three to 10, and the observed heterozygosity and expected heterozygosity per locus varied from 0.000 to 0.706 and from 0.328 to 0.807, respectively. These microsatellite markers will be useful in exploring genetic diversity and structure of A. hypargyreus . Furthermore, most loci were successfully cross-amplified in A. nitidus and A. heterophyllus , indicating that they will be of great value for genetic study across this genus.

  6. Development and characterization of EST-SSR markers for Artocarpus hypargyreus (Moraceae)1

    Science.gov (United States)

    Liu, Haijun; Tan, Weizheng; Sun, Hongbin; Liu, Yu; Meng, Kaikai; Liao, Wenbo

    2016-01-01

    Premise of the study: Polymorphic microsatellite markers were developed for Artocarpus hypargyreus (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species. Methods and Results: Based on the transcriptome data of A. hypargyreus, 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displayed clear polymorphisms across the 67 individuals from four populations of A. hypargyreus. The results showed the number of alleles per locus ranged from three to 10, and the observed heterozygosity and expected heterozygosity per locus varied from 0.000 to 0.706 and from 0.328 to 0.807, respectively. Conclusions: These microsatellite markers will be useful in exploring genetic diversity and structure of A. hypargyreus. Furthermore, most loci were successfully cross-amplified in A. nitidus and A. heterophyllus, indicating that they will be of great value for genetic study across this genus. PMID:28101438

  7. Ultrasound assisted extraction of pectin from waste Artocarpus heterophyllus fruit peel.

    Science.gov (United States)

    Moorthy, I Ganesh; Maran, J Prakash; Ilakya, S; Anitha, S L; Sabarima, S Pooja; Priya, B

    2017-01-01

    Four factors three level face centered central composite response surface design was employed in this study to investigate and optimize the effect of process variables (liquid-solid (LS) ratio (10:1-20:1ml/g), pH (1-2), sonication time (15-30min) and extraction temperature (50-70°C)) on the maximum extraction yield of pectin from waste Artocarpus heterophyllus (Jackfruit) peel by ultrasound assisted extraction method. Numerical optimization method was adapted in this study and the following optimal condition was obtained as follows: Liquid-solid ratio of 15:1ml/g, pH of 1.6, sonication time of 24min and temperature of 60°C. The optimal condition was validated through experiments and the observed value was interrelated with predicted value. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam).

    Science.gov (United States)

    Ruiz-Montañez, G; Burgos-Hernández, A; Calderón-Santoyo, M; López-Saiz, C M; Velázquez-Contreras, C A; Navarro-Ocaña, A; Ragazzo-Sánchez, J A

    2015-05-15

    The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Decline of jackfruit (Artocarpus heterophyllus incited by Phytophthora palmivora in Vietnam

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    Mai Van TRI

    2015-09-01

    Full Text Available A new disease of jackfruit (Artocarpus heterophyllus Lam. was observed in the south- eastern region of South Vietnam. Symptoms included root rot, cankers and gummosis of trunks, chlorosis, wilt, blight of leaves, defoliation, fruit brown rot, and tree death. The disease was found in 10% of surveyed farms with an incidence varying from 2% to nearly 60% of the trees. A Phytophthora species, identified as P. palmivora (Butler Butler, using the ITS1-5.8S-ITS2 region of the rDNA as a barcode gene and morphological and cultural features, was consistently isolated from symptomatic roots, fruits, trunk cankers and leaves. Koch’s postulates were fulfilled using pathogenicity tests on seedlings, leaves and detached fruits of jackfruit. To our knowledge, this is the first report of P. palmivora on jackfruit in Vietnam.

  10. A BIODEGRADABLE FILM FROM JACKFRUIT (ARTOCARPUS HETEROPHYLLUS AND DURIAN (DURIO ZIBETHINUS SEED FLOURS

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    Diah S. Retnowati

    2016-02-01

    Full Text Available The jackfruit (Artocarpus heterophyllus and durian (Durio zibethinus seeds contain high portion of amylose, which makes them potential materials for biodegradable films. The objective of this study is to develop biodegradable films composed of jackfruit and durian seed flours and glycerol as plasticizer. The films were prepared by dispersing flours in water, adding glycerol, heating the mixture, casting the solution on an acrylic plate, and drying the films at 50 ºC for 20 hours. Glycerol contributes to the flexibility of the film, while reduces the strength of the film. The Young’s modulus, tensile strength, and elongation at break increase as the ratio of jackfruit to durian seed flour increases. The films made from jackfruit and durian seed flours in this work are stronger than the film made from rice flour obtained by other researcher. Therefore, jackfruit and durian seed flours are potential raw material for biodegradable films.

  11. [Optimization of ultrasonic-assisted extraction of total flavonoids from leaves of the Artocarpus heterophyllus by response surface methodology].

    Science.gov (United States)

    Wang, Hong-wu; Liu, Yan-qing; Wang, Yuan-hong

    2011-07-01

    To investigate the ultrasonic-assisted extract on of total flavonoids from leaves of the Artocarpus heterophyllus. Investigated the effects of ethanol concentration, extraction time, and liquid-solid ratio on flavonoids yield. A 17-run response surface design involving three factors at three levels was generated by the Design-Expert software and experimental data obtained were subjected to quadratic regression analysis to create a mathematical model describing flavonoids extraction. The optimum ultrasonic assisted extraction conditions were: ethanol volume fraction 69.4% and liquid-solid ratio of 22.6:1 for 32 min. Under these optimized conditions, the yield of flavonoids was 7.55 mg/g. The Box-Behnken design and response surface analysis can well optimize the ultrasonic-assisted extraction of total flavonoids from Artocarpus heterophyllus.

  12. KARO’S LOCAL WISDOM: THE USE OF WOODY PLANTS FOR TRADITIONAL DIABETIC MEDICINES

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    Rospita Odorlina Situmorang

    2015-12-01

    Full Text Available This paper identifies the plant species used traditionally by Karo people in North Sumatra, to cure diabetes, analyses the cultural significance index of those plants for the Karo, and clarifies phytochemical contents of the plants. Data were collected using survey method from selected respondents (n=54 based on their knowledge and practices in utilising medicinal plants to cure diabetic disease. Index of Cultural Significance (ICS of plants was determined using the method proposed by Turner. Results showed that twelve woody plant species have been used to cure diabetes: loning leave (Psychotria sp., kacihe leave (Prunus accuminta Hook, umbrella tree leave (Maesopsis eminii Engl, mutamba leave (Guazuma ulmifolia Lamk, cepcepan leave (Villebrunea subescens Blume, pirdot/cepcepan lembu leave (Saurauia vulcani Korth, raru bark (Cotylelobium melanoxylo, breadfruit leave (Artocarpus altilis, salam leave (Syzygium polyanthum Wight, mahogany seed (Swietenia mahagoni (L. Jacq, cinnamon bark (Cinnamomum burmani, and yellow bamboo rod (Bambusa vulgaris Schrad. Five of those plants: loning, umbrella tree, mutamba, raru and salam have the highest cultural significance level. These five plants are highly needed in large quatities by the Karo people, so their availability in the forest should be securely conserved and protected. The plants used contained alkaloids, flavonoids, phenolics and terpenoids which can help to lower blood sugar level.

  13. Food processing methods influence the glycaemic indices of some commonly eaten West Indian carbohydrate-rich foods.

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    Bahado-Singh, P S; Wheatley, A O; Ahmad, M H; Morrison, E Y St A; Asemota, H N

    2006-09-01

    Glycaemic index (GI) values for fourteen commonly eaten carbohydrate-rich foods processed by various methods were determined using ten healthy subjects. The foods studied were round leaf yellow yam (Dioscorea cayenensis), negro and lucea yams (Dioscorea rotundata), white and sweet yams (Dioscorea alata), sweet potato (Solanum tuberosum), Irish potato (Ipomoea batatas), coco yam (Xanthosoma spp.), dasheen (Colocasia esculenta), pumpkin (Cucurbita moschata), breadfruit (Artocarpus altilis), green banana (Musa sapientum), and green and ripe plantain (Musa paradisiaca). The foods were processed by boiling, frying, baking and roasting where applicable. Pure glucose was used as the standard with a GI value of 100. The results revealed marked differences in GI among the different foods studied ranging from 35 (se 3) to 94 (se 8). The area under the glucose response curve and GI value of some of the roasted and baked foods were significantly higher than foods boiled or fried (P<0.05). The results indicate that foods processed by roasting or baking may result in higher GI. Conversely, boiling of foods may contribute to a lower GI diet.

  14. Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers.

    Science.gov (United States)

    Shyamalamma, S; Chandra, S B C; Hegde, M; Naryanswamy, P

    2008-07-22

    Artocarpus heterophyllus Lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. Yet, it has been little explored commercially due to wide variation in fruit quality. The genetic diversity and genetic relatedness of 50 jackfruit accessions were studied using amplified fragment length polymorphism markers. Of 16 primer pairs evaluated, eight were selected for screening of genotypes based on the number and quality of polymorphic fragments produced. These primer combinations produced 5976 bands, 1267 (22%) of which were polymorphic. Among the jackfruit accessions, the similarity coefficient ranged from 0.137 to 0.978; the accessions also shared a large number of monomorphic fragments (78%). Cluster analysis and principal component analysis grouped all jackfruit genotypes into three major clusters. Cluster I included the genotypes grown in a jackfruit region of Karnataka, called Tamaka, with very dry conditions; cluster II contained the genotypes collected from locations having medium to heavy rainfall in Karnataka; cluster III grouped the genotypes in distant locations with different environmental conditions. Strong coincidence of these amplified fragment length polymorphism-based groupings with geographical localities as well as morphological characters was observed. We found moderate genetic diversity in these jackfruit accessions. This information should be useful for tree breeding programs, as part of our effort to popularize jackfruit as a commercial crop.

  15. Preliminary nutritional and biological potential of Artocarpus heterophyllus L. shell powder.

    Science.gov (United States)

    Sharma, Anubhuti; Gupta, Priti; Verma, A K

    2015-03-01

    Artocarpus heterophyllus shell powder was investigated in terms of its nutritional and biological potential. A thorough examination of shell powder demonstrated its potential as a source of minerals, β carotene and dietary fiber, which were assessed gravimetrically & spectrophotometrically. This showed 3.05 ± 0.19 g 100 g(-1) DW of alkaloids followed by saponins and tannins. Three different extracts; acetone, methanol, & mix solvent were used to evaluate phenolic & flavonoid content, antioxidant & antimicrobial activity, GC/MS screening and quantitative analysis of polyphenols. Among all, the methanol extract showed highest antioxidant activity evaluated by DPPH, FRAP & ABTS assays and was significantly correlated with phenolic and flavonoid contents. Phenolic & flavonoid content was found to be 158 ± 0.34 mg (GAE) and 10.0 ± 0.64 mg (CE) respectively. The results of antimicrobial activity showed that L. monocytogenes was more susceptible to all extracts followed by other microorganisms. Catechin, ascorbic & chlorogenic acids were identified as major polyphenols analyzed by LC-MS/MS. GC/MS analysis showed that it contains a variety of compounds with different therapeutic activities. The study revealed that A. heterophyllus shell is a good source of natural antioxidants & other bioactive compounds and can be used in cosmetics, medicines and functional food application.

  16. Jacalin: a jackfruit (Artocarpus heterophyllus) seed-derived lectin of versatile applications in immunobiological research.

    Science.gov (United States)

    Kabir, S

    1998-03-15

    Jacalin, the major protein from the jackfruit (Artocarpus heterophyllus) seeds, is a tetrameric two-chain lectin (molecular mass 65 kDa) combining a heavy alpha chain of 133 amino acid residues with a light beta chain of 20-21 amino acid residues. It is highly specific for the alpha-O-glycoside of the disaccharide Thomsen-Friedenreich antigen (Gal beta1-3GalNAc), even in its sialylated form. This property has made jacalin suitable for studying various O-linked glycoproteins, particularly human IgA1. Jacalin's uniqueness in being strongly mitogenic for human CD4+ T lymphocytes has made it a useful tool for the evaluation of the immune status of patients infected with human immunodeficiency virus (HIV)-1. The abundance of source material for the production of jacalin, its ease of purification, yield and stability have made it an attractive cost-effective lectin. It has found applications in diverse areas such as the isolation of human plasma glycoproteins (IgA1, C1-inhibitor, hemopexin, alpha2-HSG), the investigation of IgA-nephropathy, the analysis of O-linked glycoproteins and the detection of tumours.

  17. Artocarpus heterophyllus seeds inhibits sexual competence but not fertility of male rats.

    Science.gov (United States)

    Ratnasooriya, W D; Jayakody, J R A C

    2002-03-01

    According to Ayurvedic literature of Sri Lanka, roasted seeds of Artocarpus heterophyllus Lam. (Family: Moraceae) has aphrodisiac activity. However, some reproductively active young men in rural areas of Sri Lanka claim that consumption of these seeds few hours prior to coitus disrupts sexual function. Because of these two conflicting claims, it was thought useful to scientifically investigate the effects of A. heterophyllus seeds on male sexual function and fertility. This was done using a seed suspension in 1% methylcellulose (SS) in rats. In a sexual behaviour study using receptive female rats, an oral administration of 500 mg/kg dose of SS markedly inhibited libido, sexual arousal, sexual vigour and sexual performance within 2 hr. Further, the treatment induced a mild erectile dysfunction. These antimasculine effects on sexual function was not evident 6 hr post treatment indicating rapid onset and offset of action. Further, these actions on the sexual behaviour was not due to general toxicity, liver toxicity, stress or reduction in blood testosterone level but due to marked sedative activity. In a mating study, SS failed to alter ejaculating competence and fertility. These results suggest that A. heterophyllous seeds do not have aphrodisiac action, at least, in rats.

  18. A novel serine protease with human fibrino(geno)lytic activities from Artocarpus heterophyllus latex.

    Science.gov (United States)

    Siritapetawee, Jaruwan; Thumanu, Kanjana; Sojikul, Punchapat; Thammasirirak, Sompong

    2012-07-01

    A protease was isolated and purified from Artocarpus heterophyllus (jackfruit) latex and designated as a 48-kDa antimicrobial protease (AMP48) in a previous publication. In this work, the enzyme was characterized for more biochemical and medicinal properties. Enzyme activity of AMP48 was strongly inhibited by phenylmethanesulfonyl fluoride and soybean trypsin inhibitor, indicating that the enzyme was a plant serine protease. The N-terminal amino acid sequences (A-Q-E-G-G-K-D-D-D-G-G) of AMP48 had no sequence similarity matches with any sequence databases of BLAST search and other plant serine protease. The secondary structure of this enzyme was composed of high α-helix (51%) and low β-sheet (9%). AMP48 had fibrinogenolytic activity with maximal activity between 55 and 60°C at pH 8. The enzyme efficiently hydrolyzed α followed by partially hydrolyzed β and γ subunits of human fibrinogen. In addition, the fibrinolytic activity was observed through the degradation products by SDS-PAGE and emphasized its activity by monitoring the alteration of secondary structure of fibrin clot after enzyme digestion using ATR-FTIR spectroscopy. This study presented the potential role to use AMP48 as antithrombotic for treatment thromboembolic disorders such as strokes, pulmonary emboli and deep vein thrombosis. Copyright © 2012 Elsevier B.V. All rights reserved.

  19. Antimicrobial activity of a 48-kDa protease (AMP48) from Artocarpus heterophyllus latex.

    Science.gov (United States)

    Siritapetawee, J; Thammasirirak, S; Samosornsuk, W

    2012-01-01

    Artocarpus heterophyllus (jackfruit) is a latex producing plant. Plant latex is produced from secretory cells and contains many intergradients. It also has been used in folk medicine. This study aimed to purify and characterize the biological activities of a protease from jackfruit latex. A protease was isolated and purified from crude latex of a jackfruit tree by acid precipitation and ion exchange chromatography. The proteolytic activities of protein were tested using gelatin- and casein-zymography. The molecular weight and isoelectric point (pl) of protein were analysed by SDS/12.5% PAGE and 2D-PAGE, respectively. Antimicrobial activity of protein was analysed by broth microdilution method. In addition, the antibacterial activity of protein against Pseudomonas aeruginosa ATCC 27853 was observed and measured using atomic force microscopy (AFM) technique. The purified protein contained protease activity by digesting gelatin- and casein-substrates. The protease was designated as antimicrobial protease-48 kDa or AMP48 due to its molecular mass on SDS-PAGE was approximately 48 kDa. The isoelectric point (pl) of AMP48 was approximately 4.2. In addition, AMP48 contained antimicrobial activities by it could inhibit the growths of Pseudomonas aeruginosa ATCC 27853 and clinical isolated Candida albicans at minimum inhibitory concentration (MIC) 2.2 mg/ml and Minimum microbicidal concentration (MMC) 8.8 mg/ml. AFM image also supported the antimicrobial activities of AMP48 by the treated bacterial morphology and size were altered from normal.

  20. Chemoprevention of Colorectal Cancer by Artocarpin, a Dietary Phytochemical from Artocarpus heterophyllus.

    Science.gov (United States)

    Sun, Guochuan; Zheng, Zongping; Lee, Mee-Hyun; Xu, Yijuan; Kang, Soouk; Dong, Zigang; Wang, Mingfu; Gu, Zhennan; Li, Haitao; Chen, Wei

    2017-05-03

    Artocarpus heterophyllus is an evergreen tree distributed in tropical regions, and its fruit (jackfruit) is well-known as the world's largest tree-borne fruit. Although A. heterophyllus has been widely used in folk medicines against inflammation, its potential in cancer chemoprevention remains unclear. Herein we identified artocarpin from A. heterophyllus as a promising colorectal cancer chemopreventive agent by targeting Akt kinase. Phenotypically, artocarpin exhibited selective cytotoxicity against human colon cancer cells. Artocarpin impaired the anchorage-independent growth capability, suppressed colon cancer cell growth, and induced a G1 phase cell cycle arrest which was followed by apoptotic as well as autophagic cell death. Mechanistic studies revealed that artocarpin directly targeted Akt 1 and 2 kinase activity evidenced by in vitro kinase assay, ex vivo binding assay as well as Akt downstream cellular signal transduction. Importantly, oral administration of artocarpin attenuated colitis-associated colorectal tumorigenesis in mice. Taken together, artocarpin, a bioactive component of A. heterophyllus, might merit investigation as a potential colorectal cancer chemopreventive agent.

  1. Chemical components and tyrosinase inhibitors from the twigs of Artocarpus heterophyllus.

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    Zheng, Zong-Ping; Chen, Sibao; Wang, Shiyun; Wang, Xia-Chang; Cheng, Ka-Wing; Wu, Jia-Jun; Yang, Dajiang; Wang, Mingfu

    2009-08-12

    An HPLC method was developed and validated to compare the chemical profiles and tyrosinase inhibitors in the woods, twigs, roots, and leaves of Artocarpus heterophyllus . Five active tyrosinase inhibitors including dihydromorin, steppogenin, norartocarpetin, artocarpanone, and artocarpesin were used as marker compounds in this HPLC method. It was discovered that the chemical profiles of A. heterophyllus twigs and woods are quite different. Systematic chromatographic methods were further applied to purify the chemicals in the twigs of A. heterophyllus. Four new phenolic compounds, including one isoprenylated 2-arylbenzofuran derivative, artoheterophyllin A (1), and three isoprenylated flavonoids, artoheterophyllin B (2), artoheterophyllin C (3), and artoheterophyllin D (4), together with 16 known compounds, were isolated from the ethanol extract of the twigs of A. heterophyllus. The structures of compounds 1-4 were elucidated by spectroscopic analysis. However, the four new compounds did not show significant inhibitory activities against mushroom tyrosinase compared to kojic acid. It was found that similar compounds, such as norartocarpetin and artocarpesin in the twigs and woods of A. heterophyllus, contributed to their tyrosinase inhibitory activity.

  2. Artocarpus heterophyllus L. seed starch-blended gellan gum mucoadhesive beads of metformin HCl.

    Science.gov (United States)

    Nayak, Amit Kumar; Pal, Dilipkumar; Santra, Kousik

    2014-04-01

    Jackfruit (Artocarpus heterophyllus Lam., family: Moraceae) seed starch (JFSS)-gellan gum (GG) mucoadhesive beads containing metformin HCl were developed through ionotropic gelation technique. The effect of GG to JFSS ratio and CaCl2 concentration on the drug encapsulation efficiency (DEE, %) and cumulative drug release at 10h (R10h, %) was optimized and analyzed using response surface methodology based on 3(2) factorial design. The optimized JFSS-GG beads containing metformin HCl showed DEE of 92.67±4.46%, R10h of 61.30±2.37%, and mean diameter of 1.67±0.27 mm. The optimized beads showed pH-dependent swelling and mucoadhesivity with the goat intestinal mucosa. The in vitro drug release from all these JFSS-GG beads containing metformin HCl was followed zero-order pattern (R(2)=0.9907-0.9975) with super case-II transport mechanism over a period of 10 h. The beads were also characterized by SEM and FTIR. The optimized JFSS-GG beads containing metformin HCl exhibited significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Antioxidant and antibacterial activities on foodborne pathogens of Artocarpus heterophyllus Lam. (Moraceae) leaves extracts.

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    Loizzo, M R; Tundis, R; Chandrika, U G; Abeysekera, A M; Menichini, F; Frega, N G

    2010-06-01

    Total water extract, ethyl acetate, and aqueous fractions from the leaves of Artocarpus heterophyllus were evaluated for phenolic content, antioxidant, and antibacterial activities against some foodborne pathogens such as E. coli, Listeria monocytogenes, Salmonella typhimurium, Salmonella enterica, Bacillus cereus, Enterococcus faecalis, and Staphylococcus aureus. The minimum inhibitory concentration (MICs) of extract and fractions determined by the agar dilution method were ranged from 221.9 microg/mL for ethyl acetate fraction to 488.1 microg/mL for total extract. In the agar diffusion method the diameters of inhibition were 12.2 for the total extract, 10.7 and 11.5 for ethyl acetate and aqueous fractions, respectively. A. heterophyllus showed significant antioxidant activity tested in different in vitro systems (DPPH, ABTS, FRAP, and Fe(2+) chelating activity assay). In particular, in DPPH assay A. heterophyllus total extract exhibited a strong antiradical activity with an IC(50) value of 73.5 microg/mL while aqueous fraction exerted the highest activity in FRAP assay (IC(50) value of 72.0 microg/mL). The total phenols content by Folin-Ciocalteau method was determined with the purpose of testing its relationship with the antioxidant and antibacterial activities.

  4. Isolation of tyrosinase inhibitors from Artocarpus heterophyllus and use of its extract as antibrowning agent.

    Science.gov (United States)

    Zheng, Zong-Ping; Cheng, Ka-Wing; To, James Tsz-Kin; Li, Haitao; Wang, Mingfu

    2008-12-01

    A new furanoflavone, 7-(2,4-dihydroxyphenyl)-4-hydroxy-2-(2-hydroxy propan-2-yl)-2, 3-dihydrofuro(3, 2-g)chromen-5-one (artocarpfuranol, 1), together with 14 known compounds, dihydromorin (2), steppogenin (3), norartocarpetin (4), artocarpanone (5), artocarpesin (6), artocarpin (7), cycloartocarpin (8), cycloartocarpesin (9), artocarpetin (10), brosimone I (11), cudraflavone B (12), carpachromene (13), isoartocarpesin (14), and cyanomaclurin (15) were isolated from the wood of Artocarpus heterophyllus. Their structures were identified by interpretation of MS,( 1)H-NMR,( 13)C-NMR, HMQC, and HMBC spectroscopic data. Among them, compounds 1-6 and 14 showed strong mushroom tyrosinase inhibitory activity with IC(50) values lower than 50 microM, more potent than kojic acid (IC(50) = 71.6 microM), a well-known tyrosinase inhibitor. In addition, extract of A. heterophyllus was evaluated for its antibrowning effect on fresh-cut apple slices. It was discovered that fresh-cut apple slices treated by dipping in solution of 0.03 or 0.05% of A. heterophyllus extract with 0.5% ascorbic acid did not undergo any substantial browning reaction after storage at room temperature for 24 h. The antibrowning effect was significantly better than samples treated with the extract (0.03 or 0.05%) or ascorbic acid (0.5%) alone. The results provide preliminary evidence supporting the potential of this natural extract as antibrowning agent in food systems.

  5. Transcriptome and selected metabolite analyses reveal points of sugar metabolism in jackfruit (Artocarpus heterophyllus Lam.).

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    Hu, Lisong; Wu, Gang; Hao, Chaoyun; Yu, Huan; Tan, Lehe

    2016-07-01

    Artocarpus heterophyllus Lam., commonly known as jackfruit, produces the largest tree-borne fruit known thus far. The edible part of the fruit develops from the perianths, and contains many sugar-derived compounds. However, its sugar metabolism is poorly understood. A fruit perianth transcriptome was sequenced on an Illumina HiSeq 2500 platform, producing 32,459 unigenes with an average length of 1345nt. Sugar metabolism was characterized by comparing expression patterns of genes related to sugar metabolism and evaluating correlations with enzyme activity and sugar accumulation during fruit perianth development. During early development, high expression levels of acid invertases and corresponding enzyme activities were responsible for the rapid utilization of imported sucrose for fruit growth. The differential expression of starch metabolism-related genes and corresponding enzyme activities were responsible for starch accumulated before fruit ripening but decreased during ripening. Sucrose accumulated during ripening, when the expression levels of genes for sucrose synthesis were elevated and high enzyme activity was observed. The comprehensive transcriptome analysis presents fundamental information on sugar metabolism and will be a useful reference for further research on fruit perianth development in jackfruit. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  6. Artonin E and Structural Analogs from Artocarpus Species Abrogates Estrogen Receptor Signaling in Breast Cancer

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    Imaobong Etti

    2016-06-01

    Full Text Available The increasing rate of mortality ensued from breast cancer has encouraged research into safer and efficient therapy. The human Estrogen receptor α has been implicated in the majority of reported breast cancer cases. Molecular docking employing Glide, Schrodinger suite 2015, was used to study the binding affinities of small molecules from the Artocarpus species after their drug-like properties were ascertained. The structure of the ligand-binding domain of human Estrogen receptor α was retrieved from Protein Data Bank while the structures of compounds were collected from PubChem database. The binding interactions of the studied compounds were reported as well as their glide scores. The best glide scored ligand, was Artonin E with a score of −12.72 Kcal when compared to other studied phytomolecules and it evoked growth inhibition of an estrogen receptor positive breast cancer cells in submicromolar concentration (3.8–6.9 µM in comparison to a reference standard Tamoxifen (18.9–24.1 µM within the tested time point (24–72 h. The studied ligands, which had good interactions with the target receptor, were also drug-like when compared with 95% of orally available drugs with the exception of Artoelastin, whose predicted physicochemical properties rendered it less drug-like. The in silico physicochemical properties, docking interactions and growth inhibition of the best glide scorer are indications of the anti-breast cancer relevance of the studied molecules.

  7. Pemanfaatan Tumbuhan Cempedak (Artocarpus champeden oleh Masyarakat Kampung Sabun Distrik Aitinyo Tengah Kabupaten Maybrat, Papua Barat

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    Ariance Juli Ross Nauw

    2016-01-01

    Full Text Available Cempedak (Artocarpus champeden is one of the non timber forest products (NTFPs from the Maybrat district. It is used as a daily food of Maybrat people. The study was conducted from June-July 2014 in Sabun Village, Aitinyo Sub district of the Maybrat Regency, West Papua Province. The objectives of this study were to explore the utilization, harvesting technique, and manufacturing process of cempedak in Sabun villages. Field observations and interviews were used to collect the data of 15 respondents. The result showed that fruits and seeds are part of of the cempedak trees, which is utilised for food. Cempedak processing were divided into 3 parts i.e. the ripe fruit, the unripe fruit, and the seed. Processing of the seed were divided into four parts i.e. boiling, steaming, frying without oil process, filling inside bamboo, and storage. Conservation efforts were conducted by planting cempedak in the garden of villagers’ house. This research also revealed that the utilization process was done for household purposes only. Therefore, the development of cempedak for an alternative food as NTFPs, is necessary to be promoted by collaboration of related stakeholders including forestry and agriculture in Maybrat Regency.

  8. Physicochemical properties and in vitro antioxidant activities of polysaccharide from Artocarpus heterophyllus Lam. pulp.

    Science.gov (United States)

    Zhu, Kexue; Zhang, Yanjun; Nie, Shaoping; Xu, Fei; He, Shuzhen; Gong, Deming; Wu, Gang; Tan, Lehe

    2017-01-02

    A water-soluble polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp (JFP-Ps) was purified and its physicochemical properties were investigated. The in vitro antioxidant activities of JFP-Ps was evaluated by measuring DPPH and OH radicals scavenging activities, as well as reducing power. The results showed that JFP-Ps contained 79.12% of total sugar, 5.83% of protein, 15.65% of uronic acid, and 15 kinds of amino acids with high levels of Asp, Glu, Val, Leu and Lys. JFP-Ps was mainly composed of Rha, Ara, Gal, Glc, Xyl and GalA, with an average molecular weight of 1668kDa. FT-IR results showed the bands at the range of 1200-850cm -1 suggested the presence of carbohydrates in JFP-Ps. The results of antioxidant activities showed that JFP-Ps exhibited strong DPPH and OH radical scavenging activities, with a relatively lower reducing power, suggesting that JFP-Ps can be exploited as effective natural antioxidant applications in medical and food industries. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. One-step synthesis of highly-biocompatible spherical gold nanoparticles using Artocarpus heterophyllus Lam. (jackfruit) fruit extract and its effect on pathogens.

    Science.gov (United States)

    Basavegowda, Nagaraj; Dhanya Kumar, Gowri; Tyliszczak, Bozena; Wzorek, Zbigniew; Sobczak-Kupiec, Agnieszka

    2015-01-01

    Novel approaches for the synthesis of gold nanoparticles (AuNPs) are of great importance due to its vast spectrum of applications in diverse fields, including medical diagnostics and therapeutics. Te presented study reports the successful AuNPs' synthesis using Artocarpus heterophyllus Lam. extract, and provides detailed characterization and evaluation of its antibacterial potential. The aim was to develop a cost-effective and environmentally friendly synthesis method of gold nanoparticles using aqueous fruit extract of Artocarpus heterophyllus Lam. as a reducing and capping agent, which has proven activity against human pathogens, such as microbial species E.coli and Streptobacillus sps. Characterizations were carried out using ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and Fourier-Transform infra-red spectroscopy (FT-IR). SEM images showed the formation of gold nanoparticles with an average size of 20-25 nm. Spectra collected while infra-red analysis contained broad peaks in ranges from 4000-400 cm -1 . It can be concluded that the fruit of Artocarpus heterophyllus Lam. can be good source for synthesis of gold nanoparticles which showed antimicrobial activity against investigated microbes, in particul E. coli, and Streptobacillus. An important outcome of this study will be the development of value-added products from the medicinal plant Artocarpus heterophyllus Lam. for the biomedical and nanotechnology-based industries.

  10. Antibacterial assay-guided isolation of active compounds from Artocarpus heterophyllus heartwoods.

    Science.gov (United States)

    Septama, Abdi Wira; Panichayupakaranant, Pharkphoom

    2015-01-01

    Preparations from Artocarpus heterophyllus Lam. (Moraceae) heartwoods are used in the traditional folk medicine for the treatment of inflammation, malarial fever, and to prevent bacterial and fungal infections. The objective of this study was to isolate pure antibacterial compounds from A. heterophyllus heartwoods. The dried and powdered A. heterophyllus heartwoods were successively extracted with the following solvents: hexane, ethyl acetate, and methanol. Each of the extracts was screened for their antibacterial activities using a disc diffusion method (10 mg/disc). Their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using a broth microdilution method. The extract that showed the strongest antibacterial activities was fractionated to isolate the active compounds by an antibacterial assay-guided isolation process. The ethyl acetate extract exhibited the strongest antibacterial activities against Streptococcus mutans, S. pyogenes, and Bacillus subtilis with MIC values of 78, 39, and 9.8 µg/mL, respectively. Based on an antibacterial assay-guided isolation, four antibacterial compounds: cycloartocarpin (1), artocarpin (2), artocarpanone (3), and cyanomaclurin (4) were purified. Among these isolated compounds, artocarpin exhibited the strongest antibacterial activity against Gram-positive bacteria, including S. mutans, S. pyogenes, B. subtilis, Staphylococcus aureus, and S. epidermidis with MICs of 4.4, 4.4, 17.8, 8.9, and 8.9 µM, respectively, and MBCs of 8.9, 8.9, 17.8, 8.9, and 8.9 µM, respectively, while artocarpanone showed the strongest activity against Escherichia coli, a Gram-negative bacteria with MIC and MBC values of 12.9 and 25.8 µM, respectively. Only artocarpin showed inhibitory activity against Pseudomonas aeruginosa with an MIC of 286.4 µM.

  11. Effect of CMC and arabic gum in the manufacture of jackfruit velva (Artocarpus heterophyllus)

    Science.gov (United States)

    Yudhistira, B.; Riyadi, N. H.; Pangestika, A. D.; Pertiwi, S. R.

    2018-03-01

    Velva is one type of frozen dessert which is made from fruit/vegetable with ice cream maker, low fat and high fiber content. Jackfruit is a raw material for the manufacture of velva because of the high fiber content of 2.31 gr. The use of a stabilizers combination of CMC and arabic gum in the manufacture of velva will provide a better gel mix than single use. The purpose of this research is to know the influence of variation of CMC and arabic gum stabilizer on the characteristics (physical, chemical, and sensory) of jackfruit velva (Artocarpus heterophyllus) and determine variations in the most appropriate combinations of stabilizers to produce jackfruit velva with the best quality. This research applied Completely Randomized Design consist of one factor which is the combination of CMC and arabic gum levels in the making of jackfruit velva with two replicates and two replications of the analysis. The data obtained then analyzed statistically using one way analysis of variance (ANOVA), when there is a significant difference, then followed by Duncan’s Multiple Range Test (DMRT) at significance level of 0.05. The results of this study concluded that the jackfruit velva with the addition of various concentrations of CMC and arabic gum is significantly affecting the taste, texture and overall parameters, but no significant difference on the color and flavor parameters of jackfruit velva. Based on the results of physical characteristics, chemical and sensory jackfruit velva with the addition of a stabilizing concentration of CMC and arabic gum 1: 1 result in best jackfruit velva. The best jackfruit velva with stabilizing the concentration of CMC and arabic gum 1: 1 contains a water content of 61.95%, dietary fiber 2.231%, total dissolved solids 20.38 °Brix, overrun 19.709%, meltdown 28.215 minutes. As for the color attribute score 3.72; Taste 4; flavor 3.60; Texture 3.68, and overall 3.88.

  12. Purification and characterization of a trypsin inhibitor from the seeds of Artocarpus heterophyllus Lam.

    Science.gov (United States)

    Lyu, Junchen; Liu, Yuan; An, Tianchen; Liu, Yujun; Wang, Manchuriga; Song, Yanting; Zheng, Feifei; Wu, Dan; Zhang, Yingxia; Deng, Shiming

    2015-05-01

    A proteinaceous inhibitor against trypsin was isolated from the seeds of Artocarpus heterophyllus Lam. by successive ammonium sulfate precipitation, ion-exchange, and gel-filtration chromatography. The trypsin inhibitor, named as AHLTI (A. heterophyllus Lam. trypsin inhibitor), consisted of a single polypeptide chain with a molecular weight of 28.5 kDa, which was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel-filtration chromatography. The N-terminal sequence of AHLTI was DEPPSELDAS, which showed no similarity to other known trypsin inhibitor sequence. AHLTI completely inhibited bovine trypsin at a molar ratio of 1:2 (AHLTI:trypsin) analyzed by native polyacrylamide gel electrophoresis, inhibition activity assay, and gel-filtration chromatography. Moreover, kinetic enzymatic studies were carried out to understand the inhibition mechanism of AHLTI against trypsin. Results showed that AHLTI was a competitive inhibitor with an equilibrium dissociation constant (Ki) of 3.7 × 10(-8) M. However, AHLTI showed weak inhibitory activity toward chymotrypsin and elastase. AHLTI was stable over a broad range of pH 4-8 and temperature 20-80°C. The reduction agent, dithiothreitol, had no obvious effect on AHLTI. The trypsin inhibition assays of AHLTI toward digestive enzymes from insect pest guts in vitro demonstrated that AHLTI was effective against enzymes from Locusta migratoria manilensis (Meyen). These results suggested that AHLTI might be a novel trypsin inhibitor from A. heterophyllus Lam. belonging to Kunitz family, and play an important role in protecting from insect pest. © The Author 2015. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

  13. Proximate composition, mineral content and functional properties of Tarap (Artocarpus odoratissimus seed flour

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    Noorfarahzilah, M.

    2017-06-01

    Full Text Available Tarap seed (Artocarpus odoratissimus is one of the popular snacks among Borneo Island people especially in Sabah, Malaysia. Their flesh and seed are such a potential food source but they are not fully exploited. Therefore, this study was conducted to determine its nutritional composition, mineral content and functional properties of Tarap seed flour (TSF. The proximate analysis (protein, carbohydrate, fat, crude fiber, ash, moisture, minerals content and functional properties (powder particle size, bulk density, oil and water absorption capacity, emulsion capacity, foaming ability, least gelling concentration, swelling ability and pasting properties were determined. The proximate analysis results obtained showed that TSF had 49.65% carbohydrate, 15.60% crude fat, 12.3% crude fibers, 8.8% crude protein, 1.17 % ash and 12.5% moisture. Most abundant mineral found in Tarap flour was potassium, followed by magnesium, calcium, and natrium. The results showed that TSF has an average particle size of 166.02μm with low bulk density (0.57g/cm3 as compared to wheat flour. Water and oil absorption capacities of TSF were 2.61 and 1.69g/g, respectively, while the efficiency of emulsification was 14.8%. Least gelation concentration was found to be 12% while foaming ability was 7%. Swelling ability results showed a steady increment from 60°C to 70°C but sharp increased observed from 70°C to 80°C. From the results presented, it can be concluded that TSF has a great potential for use in the formulation of composite flours to be used as ingredients in the food industry.

  14. Kinetika Hidrolisis Pati Biji Nangka (Artocarpus heterophyllus Menggunakan Katalisator Asam Klorida (HCl

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    Sri Endang

    2014-07-01

    Full Text Available The research of kinetic hydrolysis from the starch of jackfruit seed (Artocarpus heterophyllus used clorid acid catalyst (HCl aims to know the influence of variation of concentration HCl catalyst which give the maximum result of hydrolysis of the starch of jackfruit seed, determining the optimum concentration of HCl catalyst that produces the maximum value of the reaction rate constants, and determine the value of the reaction rate constant of hydrolysis the starch of jackfruit seed using catalyst. The method of this research has done in two stages that are the determination of the optimum catalyst concentration of HCl hydrolysis reaction of the starch of jackfruit seed using various concentration of catalyst HCl 0,5 M; 1,0 M; 1,5 M; 2,0 M and 2,5 M at the optimum temperature and stirring time (90oC during 70 minutes. Hydrolysis followed by neutralization process using sodium hydroxide solution and evaporated to get the form of glucose concentrated, that glucose is analyzed by qualitative and quantitative with Benedict experiment and fenol sulphate acid method, based on maximum degree of glucose which is gotten from the result of hydrolysis the starch of jackfruit seed variation concentration oh HCl is in HCl 1,5 M concentration with degree of glucose (% weight is 7,54% with percentage of starch conversion is 83,21%. Second step is determining the value of hydrolysis constant rate reaction which use time variation (30, 40,50, 60 and 70 minutes in 70 minutes optimum operation condition, 90oC te mperature and concentration catalyst of HCl 1,5 M. Based on statistic calculation date of ANOVA is gained F hitung < F tabel so Ho receive and reject HI  which shows that there is not influence catalyst variation concentration of HCl to the result of hydrolysis the starch of jackfruit seed which is gained. The result of the research shows that hydrolysis of kinetic of the starch of jackfruit seeduse HCl catalyst is reaction of the first apparent orde with

  15. 3-Prenyl luteolin, a new prenylated flavone with melanin biosynthesis inhibitory activity from wood of Artocarpus heterophyllus.

    Science.gov (United States)

    Arung, Enos Tangke; Shimizu, Kuniyoshi; Tanaka, Hiroyuki; Kondo, Ryuichiro

    2010-09-01

    In our efforts to find new whitening agent from natural resources, we focused on wood of Artocarpus heterophyllus which shows anti-melanogenesis activity. By activity-guided fractionation of A. heterophyllus wood extract, a new prenylated flavonoid, 3-prenyl luteolin (1) was isolated. The IC(50) of mushroom tyrosinase inhibitory activity of 1 was 76.3 microM. The results of the comparison with that of luteolin showed the prenyl substituent at C-3 position of 1 play an important role for revealing tyrosinase inhibition. In melanin formation inhibition on B16 melanoma cells, IC(50) of 1 was 56.7 microM with less cytotoxicity. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  16. Isoprenoid-substituted flavonoids from wood of Artocarpus heterophyllus on B16 melanoma cells: cytotoxicity and structural criteria.

    Science.gov (United States)

    Arung, Enos Tangke; Yoshikawa, Keisuke; Shimizu, Kuniyoshi; Kondo, Ryuichiro

    2010-03-01

    As a result of cytotoxicity-guided fractionation, nine flavonoids, artocarpin (1), cudraflavone C (2), 6-prenylapigenin (3), kuwanon C (4), norartocarpin (5), albanin A (6), cudraflavone B (7), brosimone I (8) and artocarpanone (9) were identified from the methanol extract of the wood of Artocarpus heterophyllus, known commonly as Nangka in Indonesia. A structure-activity investigation of the effect of these isolated compounds (1-9) and structurally related compounds on B16 melanoma cells indicated that isoprenoid moiety substitutions in flavonoids enhance their cytotoxicity, and that the position of attachment and the number of isoprenoid-substituent moieties per molecule influence flavonoid cytotoxicity. 2009 Elsevier B.V. All rights reserved.

  17. Evaluation of in vitro antioxidant potential anti-inflammatory activity and melanogenesis inhibition of Artocarpus hirsutus Lam. extracts

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    Mahadeva Nayak

    2017-01-01

    Full Text Available Artocarpus hirsutus Lam. belongs to Moraceae family and is endemic to Western Ghats and Kerala in India. This species is found to be effective in traditional medicine for the treatment of ulcer diarrhea and pimples. However extensive biological evaluation on each component of this specific species rarely appears in the literature which restricts its applicability as medicinal herb. The leaf bark and wood of Artocarpus hirsutus Lam. were separately extracted with hot ethanol. The wood extract was further fractionated to isolate major active molecule whose structure was determined from its NMR spectra and LCMS analysis. All the extracts of A. hirsutus Lam. were then studied in vitro to evaluate their potential on tyrosinase inhibition free radical scavenging activity by 11-Diphenyl-2-picrylhydrazyl DPPH method and oxygen radical absorbance capacity ORAC. Furthermore their effects on melanogenesis inhibition were also evaluated by using murine melanoma cells. Activity guided fractionation of wood extract yielded a pure molecule that was characterized as oxyresveratrol. It was observed that antioxidant activity was higher in wood extract compared to the leaf and bark extracts. Isolated pure oxyresveratrol exhibited a significant antioxidant potential with ORAC value of 366532570 mol Trolox equivalentg and having an IC50 of 4.3 gmL for DPPH free radical scavenging activity. This molecule was found to be effective for the tyrosinase inhibition with an IC50 of 0.1 gmL and melanogenesis inhibition in cultured melanoma cells by 44.62 at 0.2 gmL. Oxyresveratrol also exhibited significant inhibition of lipopolysaccharide LPS induced tumour necrosis factor alpha TNF-amp945 secretion from J774A1 murine macrophage cell lines. This study provides substantial evidence for the presence of oxyresveratrol in the wood of A. hirsutus Lam. with promising anti-inflammatory antioxidant and skin lightening property.

  18. One-step synthesis of highly-biocompatible spherical gold nanoparticles using [i]Artocarpus heterophyllus[/i] Lam. (jackfruit fruit extract and its effect on pathogens

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    Nagaraj Basavegowda

    2015-02-01

    Full Text Available [b]Introduction[/b]. Novel approaches for the synthesis of gold nanoparticles (AuNPs are of great importance due to its vast spectrum of applications in diverse fields, including medical diagnostics and therapeutics. Te presented study reports the successful AuNPs’ synthesis using [i]Artocarpus heterophyllus[/i] Lam. extract, and provides detailed characterization and evaluation of its antibacterial potential. [b]Objective[/b]. The aim was to develop a cost-effective and environmentally friendly synthesis method of gold nanoparticles using aqueous fruit extract of [i]Artocarpus heterophyllus[/i] Lam. as a reducing and capping agent, which has proven activity against human pathogens, such as microbial species [i]E.coli[/i] and [i]Streptobacillus[/i] sps. [b]Materials and method[/b]. Characterizations were carried out using ultraviolet-visible (UV-Vis spectrophotometry, scanning electron microscopy (SEM, energy dispersive X-ray and Fourier-Transform infra-red spectroscopy (FT-IR. [b]Results[/b]. SEM images showed the formation of gold nanoparticles with an average size of 20–25 nm. Spectra collected while infra-red analysis contained broad peaks in ranges from 4000–400 cm -1 . [b]Conclusions[/b]. It can be concluded that the fruit of [i]Artocarpus heterophyllus[/i] Lam. can be good source for synthesis of gold nanoparticles which showed antimicrobial activity against investigated microbes, in particul [i]E. coli[/i], and [i]Streptobacillus[/i]. An important outcome of this study will be the development of value-added products from the medicinal plant [i]Artocarpus heterophyllus[/i] Lam. for the biomedical and nanotechnology-based industries.

  19. Effect of pH fermentation on production bioethanol from jackfruit seeds (Artocarpus heterophyllus) through separate fermentation hydrolysis method

    Science.gov (United States)

    Arif, A. R.; Natsir, H.; Rohani, H.; Karim, A.

    2018-03-01

    Bioethanol is one of the alternative energy sourced from natural products containing carbohydrates through hydrolysis and fermentation process. Jackfruit seeds is one of the feedstock that contain high carbohydrate content but less utilized. The aims of this study to determine the effect of pH hydrolysis in the process of production bioethanol from jackfruit seeds (Artocarpus heterophyllus) through separate fermentation hydrolysis (SHF) method. The hydrolysis process uses H2SO4 as a hydrolyzing agent. The fermentation process used Saccharomyces cereviceae as a fermentor with a variation of pH 2,3 4 and 5 for 70 hours. The results showed that glucose content of 75% and pH 3 was the optimum pH of fermentation with the content of bioethanol 57.94%. The fermentation stage has an important role in increasing the levels of glucose and bioethanol in linear. The content of glucose and bioethanol of jackfruit seeds showed a great potential for development as the feedstock in bioethanol production.

  20. Purification and characterization of a heteromultimeric glycoprotein from Artocarpus heterophyllus latex with an inhibitory effect on human blood coagulation.

    Science.gov (United States)

    Siritapetawee, Jaruwan; Thammasirirak, Sompong

    2011-01-01

    Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XI(a) and α-XII(a).

  1. Antioxidant Potential of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Cultivated on Artocarpus heterophyllus Sawdust Substrate in India.

    Science.gov (United States)

    Rani, P; Lal, Merlin Rajesh; Maheshwari, Uma; Krishnan, Sreeram

    2015-01-01

    The artificial cultivation of Ganoderma lucidum (MTCC1039) using Artocarpus heterophyllus as sawdust substrate was optimized and free radical scavenging activities of the generated fruiting bodies were investigated. The choice of A. heterophyllus as substrate was due to its easy availability in South India. Sawdust supplemented with dextrose medium yielded better spawn hyphae and early fruiting body initiation (15 days). The biological yield obtained was 42.06 ± 2.14 g/packet and the biological efficiency was 8.41 ± 0.48%. Both aqueous and methanolic extracts of fruiting body were analyzed for radical scavenging activity. Methanolic extract showed maximum scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (IC50 = 290 μg/ml) and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid (IC50 = 580 μg/ml), whereas aqueous extract had better scavenging for ferric reducing antioxidant power (IC50 = 5 μg/ml). Total phenolic content and total antioxidant capacity were significantly higher in methanolic extract (p < 0.01). A positive correlation existed between the phenolic content and antioxidant activity. Our results indicated that fruiting bodies of G. lucidum cultivated in sawdust medium possess antioxidant property, which can be exploited for therapeutic application.

  2. Investigation of active biomolecules involved in the nucleation and growth of gold nanoparticles by Artocarpus heterophyllus Lam leaf extract

    Science.gov (United States)

    Jiang, Xinde; Sun, Daohua; Zhang, Genlei; He, Ning; Liu, Hongyu; Huang, Jiale; Odoom-Wubah, Tareque; Li, Qingbiao

    2013-06-01

    The effects of different biomolecules in Artocarpus heterophyllus Lam leaf extract on the morphology of obtained gold nanoparticles were investigated in this study. The results indicated that reducing sugars, flavones, and polyphenols consisting of about 79.8 % dry weight of the leaf extract were mainly involved in providing the dual function of reduction and the size/shape control during the biosynthesis. The gold nanoparticles present included 64 ± 10 nm nanospheres, 131 ± 18 nm nanoflowers, and 347 ± 136 nm (edge length) nanoplates and they were synthesized using the main content of reducing sugars, flavones, and polyphenols, respectively, after they were desorbed by the AB-8 macroporous adsorption resin column. Particularly, flower-like and triangular/hexagonal gold nanoparticles with a yield more than 80 % were obtained. Possible shape-directed agents for the nucleation and growth were characterized by FTIR, it can be seen that ketones were bound on the surface of the spherical and flower-like GNPs, while both the ketones and carbonyls bound on the Au {111} plane this may have favored the formation of the twin defects, which are very essential for nanoplates' formation.

  3. Effects of seed mass on seedling success in Artocarpus heterophyllus L., a tropical tree species of north-east India

    Science.gov (United States)

    Khan, M. L.

    2004-03-01

    I examined the effects of seed mass on performance of seedlings of Artocarpus heterophyllus L. (Moraceae), a large evergreen late successional shade-tolerant tree species in three contrasting light conditions. Seed mass varied many fold from 1.5 to 14 g in A. heterophyllus. Germination and germination time showed a significant correlation with seed mass. Germination differed significantly among three light regimes (50%, 25% and 3%). Seed mass and light level significantly affected seedling survival. The seedlings that emerged from large seeds survived better than those from small seeds under all light regimes. Survival of seedlings was maximum in 25% light regime for all seed mass classes but did not differ significantly from that at 50% light regime. Survival was significantly lower in 3% light as compared to 50% and 25% light regimes. Seedling vigor (expressed in terms of seedling height, leaf area and dry weight) was also significantly affected by seed mass and light regimes. Seedlings that emerged from larger seeds and grew under 50% light regime produced the heaviest seedlings, while those resulting from smaller seeds and grown under 3% light regime produced the lightest seedlings. Resprouting capacity of seedlings after clipping was significantly affected by seed mass and light regime. Seedlings emerging from larger seeds were capable of resprouting several times successively. Resprouting was more pronounced under 50% and 25% light regimes as compared to 3% light. Success of A. heterophyllus regeneration appears to be regulated by an interactive effect of seed mass and light regime.

  4. Ultrasound assisted extraction of natural dye from jackfruit's wood (Artocarpus heterophyllus): The effect of ethanol concentration as a solvent

    Science.gov (United States)

    Febriana, Ike Dayi; Gala, Selfina; Mahfud, Mahfud

    2017-05-01

    Azo dye are synthetic organic dyes which has an azo group (- N = N -) as chromophore. Azo dye is resistand to decomposition process and harmfull for the environment and human being. Natural dye can be used as substitution of azo dye at textile industry. Natural dye are eco - friendly and can be applied for dyeing of fibrous material. Natural dye can be obtained from natural origin such as leaves, wood, or roots. The wood of jackfruit (Artocarpus heterophyllus) can used as natural source of natural dye. Ultrasound assisted extraction (UAE) is a new method that can be used to extract natural dye from jackfruit's wood. The aim of this research are to study about influence of ethanol concentration as solvent and extraction kinetic. Jackfruit's wood dust from sawmill used for the experimentation were sifted by sieve 35 mesh. Ethanol 96% used as solvent of this experiment and varied the concentration in volume to volume ratio (v/v). Experiment were carried out from 20 to 50 minutes. The result of this experiment shows that ethanol concentration influenced yield of extraction from jackfruit's wood. Concentration of ethanol will be affected polarity of solvent. The Peleg model was used to describe about kinetic model of natural dye extraction. Value of k1 and k2 constant are 0.003835 and 0.04186 respectively.

  5. Batch and continuous (fixed-bed column) biosorption of crystal violet by Artocarpus heterophyllus (jackfruit) leaf powder.

    Science.gov (United States)

    Saha, Papita Das; Chakraborty, Sagnik; Chowdhury, Shamik

    2012-04-01

    In this study, batch and fixed-bed column experiments were performed to investigate the biosorption potential of Artocarpus heterophyllus (jackfruit) leaf powder (JLP) to remove crystal violet (CV) from aqueous solutions. Batch biosorption studies were carried out as a function of solution pH, contact time, initial dye concentration and temperature. The biosorption equilibrium data showed excellent fit to the Langmuir isotherm model with maximum monolayer biosorption capacity of 43.39 mg g(-1) at pH 7.0, initial dye concentration=50 mg L(-1), temperature=293 K and contact time=120 min. According to Dubinin-Radushkevich (D-R) isotherm model, biosorption of CV by JLP was chemisorption. The biosorption kinetics followed the pseudo-second-order kinetic model. Thermodynamic analysis revealed that biosorption of CV from aqueous solution by JLP was a spontaneous and exothermic process. In order to ascertain the practical applicability of the biosorbent, fixed-bed column studies were also performed. The breakthrough time increased with increasing bed height and decreased with increasing flow rate. The Thomas model as well as the BDST model showed good agreement with the experimental results at all the process parameters studied. It can be concluded that JLP is a promising biosorbent for removal of CV from aqueous solutions. Copyright © 2011 Elsevier B.V. All rights reserved.

  6. Comparative study of the chemical composition and mineral element content of Artocarpus heterophyllus and Treculia africana seeds and seed oils.

    Science.gov (United States)

    Ajayi, Ibironke Adetolu

    2008-07-01

    A comparative study of Artocarpus heterophyllus and Treculia africana seeds, both of Moraceae family, was carried out to establish their chemical compositions and evaluate their mineral element content in order to investigate the possibility of using them for human and or animal consumption and also to examine if there is a relationship between the properties of these seeds. A. heterophyllus and T. africana are rich in protein; their protein contents are higher than those from high protein animal sources such as beef and marine fishes. Both seeds have high carbohydrate content and could act as source of energy for animals if included in their diets. The oil contents of the seeds are 11.39% and 18.54% for A. heterophyllus and T. africana, respectively. The oils are consistently liquid at room temperature. The results of the physicochemical properties of the two seeds are comparable to those of conventional oil seeds such as groundnut and palm kernel oils and could be useful for nutritional and industrial purposes. The seeds were found to be good sources of mineral elements. The result revealed potassium to be the prevalent mineral elements which are 2470.00 ppm and 1680.00 ppm for A. heterophyllus and T. africana, respectively followed by sodium, magnesium and then calcium. They also contain reasonable quantity of iron, in particular A. heterophyllus 148.50 ppm.

  7. Ekstraksi Kayu Nangka (Artocarpus heterophyllus lam dengan Pelarut Etanol sebagai Pewarna Tekstil Menggunakan Metode Microwave-Assisted Extraction

    Directory of Open Access Journals (Sweden)

    Dhaniar Rulandri Widoretno

    2017-01-01

    Full Text Available Seiring berkembangnya teknologi menyebabkan kebanyakan industri tekstil di Indonesia lebih memilih menggunakan pewarna sintetis pada proses pewarnaan kain. Pada kenyataannya pewarna sintetis dapat berdampak negatif karena bersifat toxic bagi kesehatan pekerja dan lingkungan. Untuk mengatasi masalah tersebut, salah satu cara yang dapat dilakukan adalah dengan penggunaan zat warna alami. Salah satu potensi yang belum termanfaatkan di Indonesia adalah limbah kayu nangka dari industri meubel, kayu nangka sendiri mengandung zat warna yang memberikan pigmen warna kuning sehingga dapat dimanfaatkan sebagai alternatif sumber bahan pewarna alami. Dalam penelitian ini bahan yang akan diekstraksi adalah kayu nangka (Artocarpus heterophyllus lam dengan ukuran serbuk antara 35 mesh – 60 mesh. Proses ekstraksi dilakukan dengan menggunakan pelarut etanol 96% sebanyak 200 mL. Ekstraksi dengan menggunakan metode Microwave-Assisted Extraction dilakukan pada kondisi operasi yang berbeda, yakni meliputi perbandingan rasio bahan terhadap pelarut (0,02; 0,04; 0,06; 0,08; 0,1 g/mL, daya microwave (100; 264; 400; 600: 800 watt, serta waktu ekstraksi (10; 20; 30; 40; 50; 60; 70: 80; 90 menit. Berdasarkan hasil penelitian, diperoleh yield optimum sebesar 3,39% pada daya 400 watt, 3,67% pada rasio bahan terhadap pelarut 0,02 g/mL, dan 3,49% pada waktu ekstraksi 30 menit dengan daya microwave 600 watt. Hasil pengujian pewarnaan pada tekstil menunjukkan bahwa pewarna alami kayu nangka dapat digunakan sebagai pewarna pewarna tekstil karena dapat memberikan hasil pewarnaan yang permanen.

  8. Investigation of active biomolecules involved in the nucleation and growth of gold nanoparticles by Artocarpus heterophyllus Lam leaf extract

    Energy Technology Data Exchange (ETDEWEB)

    Jiang Xinde; Sun Daohua, E-mail: sdaohua@xmu.edu.cn; Zhang Genlei; He Ning; Liu Hongyu; Huang Jiale; Odoom-Wubah, Tareque; Li Qingbiao, E-mail: kelqb@xmu.edu.cn [Xiamen University, Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Fujian Provincial Key Laboratory of Chemical Biology (China)

    2013-06-15

    The effects of different biomolecules in Artocarpus heterophyllus Lam leaf extract on the morphology of obtained gold nanoparticles were investigated in this study. The results indicated that reducing sugars, flavones, and polyphenols consisting of about 79.8 % dry weight of the leaf extract were mainly involved in providing the dual function of reduction and the size/shape control during the biosynthesis. The gold nanoparticles present included 64 {+-} 10 nm nanospheres, 131 {+-} 18 nm nanoflowers, and 347 {+-} 136 nm (edge length) nanoplates and they were synthesized using the main content of reducing sugars, flavones, and polyphenols, respectively, after they were desorbed by the AB-8 macroporous adsorption resin column. Particularly, flower-like and triangular/hexagonal gold nanoparticles with a yield more than 80 % were obtained. Possible shape-directed agents for the nucleation and growth were characterized by FTIR, it can be seen that ketones were bound on the surface of the spherical and flower-like GNPs, while both the ketones and carbonyls bound on the Au {l_brace}111{r_brace} plane this may have favored the formation of the twin defects, which are very essential for nanoplates' formation.

  9. Investigation of active biomolecules involved in the nucleation and growth of gold nanoparticles by Artocarpus heterophyllus Lam leaf extract

    International Nuclear Information System (INIS)

    Jiang Xinde; Sun Daohua; Zhang Genlei; He Ning; Liu Hongyu; Huang Jiale; Odoom-Wubah, Tareque; Li Qingbiao

    2013-01-01

    The effects of different biomolecules in Artocarpus heterophyllus Lam leaf extract on the morphology of obtained gold nanoparticles were investigated in this study. The results indicated that reducing sugars, flavones, and polyphenols consisting of about 79.8 % dry weight of the leaf extract were mainly involved in providing the dual function of reduction and the size/shape control during the biosynthesis. The gold nanoparticles present included 64 ± 10 nm nanospheres, 131 ± 18 nm nanoflowers, and 347 ± 136 nm (edge length) nanoplates and they were synthesized using the main content of reducing sugars, flavones, and polyphenols, respectively, after they were desorbed by the AB-8 macroporous adsorption resin column. Particularly, flower-like and triangular/hexagonal gold nanoparticles with a yield more than 80 % were obtained. Possible shape-directed agents for the nucleation and growth were characterized by FTIR, it can be seen that ketones were bound on the surface of the spherical and flower-like GNPs, while both the ketones and carbonyls bound on the Au {111} plane this may have favored the formation of the twin defects, which are very essential for nanoplates’ formation.

  10. Effect of Artocarpus communis Extract on UVB Irradiation-Induced Oxidative Stress and Inflammation in Hairless Mice

    Directory of Open Access Journals (Sweden)

    Feng-Lin Yen

    2013-02-01

    Full Text Available Administration of antioxidants and anti-inflammatory agents is an effective strategy for preventing ultraviolet (UV irradiation-induced skin damage. Artocarpus communis possesses several pharmacological activities, such as antioxidant, anticancer and anti-inflammation. However, the photoprotective activity of methanol extract of A. communis heartwood (ACM in ultraviolet irradiation-induced skin damage has not yet been investigated. The present study was performed using ultraviolet absorption, histopathological observation, antioxidant and anti-inflammation assays to elucidate the mechanism of the photoprotective activity of ACM. Our results indicated that ACM displayed a UVA and UVB absorption effect and then effectively decreased scaly skin, epidermis thickness and sunburn cells during ultraviolet irradiation in hairless mice. ACM not only decreased ultraviolet irradiation-mediated oxidative stress, including lowering the overproduction of reactive oxygen species and lipid peroxidation (p < 0.05, but also reduced the levels of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α and interleukin 1β. Additionally, ACM can decrease the synthesis of cytosolic phospholipase A2, cyclooxygenase, inducible nitric oxide synthase and vascular cell adhesion molecular-1 via inhibiting TNF-α-independent pathways (p < 0.05 in UVB-mediated inflammation and formation of sunburn cells. Consequently, we concluded that ACM extract has a photoprotective effect against UVB-induced oxidative stress and inflammation due to its sunscreen property, and its topical formulations may be developed as therapeutic and/or cosmetic products in further studies.

  11. Structure-activity relationship of prenyl-substituted polyphenols from Artocarpus heterophyllus as inhibitors of melanin biosynthesis in cultured melanoma cells.

    Science.gov (United States)

    Arung, Enos Tangke; Shimizu, Kuniyoshi; Kondo, Ryuichiro

    2007-09-01

    A series of prenylated, flavone-based polyphenols, compounds 1-8, were isolated from the wood of Artocarpus heterophyllus. These compounds, which have previously been shown not to inhibit tyrosinase activity, were found to be active inhibitors of the in vivo melanin biosynthesis in B16 melanoma cells, with little or no cytotoxicity. To clarify the structural requirement for inhibition, some structure-activity relationships were studied, in comparison with related compounds lacking prenyl side chains. Our experiments indicate that both prenyl and OH groups, as well as the type of substitution pattern, are crucial for the inhibition of melanin production in B16 melanoma cells.

  12. Nutrient composition, energy value and residual anti- nutritional ...

    African Journals Online (AJOL)

    ONOS

    2010-07-05

    Jul 5, 2010 ... The proximate composition, carbohydrate fraction, minerals and residual ... energy-rich fruits like bread fruit (Artocarpus altilis) that ... samples were dried in an oven at 60°C for 72 h, bulked and milled ... Composition of the reference and test diets used in the apparent metabolizable energy assay (100%).

  13. Evaluation de l'activité antioxydante des extraits hydro-ethanoliques ...

    African Journals Online (AJOL)

    activity of the hydro-ethanolic leaf and bark extracts of Piliostigma thonningii. The polyphenol content was determined with ... scavenger activity with respective IC50 values 39.3 ± 1.00 μg / ml and 109 ± 6.25 μg / ml. Reducing power of bark extract was ..... arginase inhibitory potentials of extracts from. Artocarpus altilis,. Ficus.

  14. Anti-Cancer Properties of Diethylether Extract of Wood from Sukun ...

    African Journals Online (AJOL)

    Purpose: To evaluate the anti-cancer properties of the diethylether extract of Sukun (Artocarpus altilis) wood. Methods: The extract was tested in human T47D breast cancer cells and examined for its effect on cell viability, nuclear morphology and sub-G1 formation. Cell viability was determined by microculture tetrazolium ...

  15. Jackfruit (Artocarpus heterophyllus lamk) wood waste as a textile natural dye by micowave-assisted extraction method

    Science.gov (United States)

    Qadariyah, Lailatul; Gala, Selfina; Widoretno, Dhaniar Rulandri; Kunhermanti, Delita; Bhuana, Donny S.; Sumarno, Mahfud, Mahfud

    2017-05-01

    The development of technology causes most of textile industries in Indonesia prefer to use synthetic dyes in the fabric dyeing process. In fact, synthetic dyes is able to have negative effect since it is is toxic to the health of workers and environment. To resolve this issues, one way to do is to use natural dyes. One of untapped potential in Indonesia is wood waste of jackfruit from furniture industry. Jackfruit wood itself containing dyestuffs which gives yellow color pigment so that it can be used as an alternative source of natural dyes. The purpose of this research is to study the effect of extraction time, mass to solvent volume ratio, and microwave power to yield of dyes. The extract of dye analyzed by UV-Visible Spectrophotometer and GC-MS, along the coloring and endurance tests of natural dyes on fabric and compare it with synthetic dyes. In this research, material is going to be extracted is the wood of jackfruit (Artocarpus heterophyllus lamk) with material size between 35 mesh - 60 mesh. The extraction process is done by using ethanol 96%. Extraction using MAE is carried out at the ratio of materials to solvent of 0,02-0,1 g/mL, the microwave power of 100-800 Watt, and the extraction time of 10-90 minutes. The conclusion is at microwave power of 400 Watt, material to solvent ratio of the 0,02 g/mL, the yield is 3,39% while at microwave power of 600 Watt, material to solvent ratio of the 0,02 g/mL, the yield is 3,67% with extraction time of 30 minutes. The highest recovery from ethanol 96% solvent is 60,41%. The result of UV-Vis Spectrophotometry and GC-MS test show that there is a chromophore compound in the extract of natural dye. The test results show the natural dyes of jackfruit wood can be used to coloring on the textile because it can gives staining result permanently.

  16. Ameliorative Activity of Ethanol Extract of Artocarpus heterophyllus Stem Bark on Pancreatic β-Cell Dysfunction in Alloxan-Induced Diabetic Rats.

    Science.gov (United States)

    Ajiboye, Basiru O; Ojo, Oluwafemi A; Adeyonu, Oluwatosin; Imiere, Oluwatosin D; Fadaka, Adewale O; Osukoya, Adetutu O

    2017-10-01

    This study sought to investigate the ameliorative effects of ethanol extract Artocarpus heterophyllus (EAH) in alloxan-induced diabetic rats. The rats were divided into 6 groups, with groups 1 and 2 serving as nondiabetic and diabetic control, respectively; group 3 serving as diabetic rats treated with 5 mg/kg glibenclamide; and groups 4 to 6 were diabetic rats treated with 50, 100, and 150 mg/kg of EAH, respectively. Assays determined were serum insulin, lipid peroxidation, and antioxidant enzyme activities. EAH stem bark reduced fasting blood glucose and lipid peroxidation levels and increased serum insulin levels and activities of antioxidant enzymes. Data obtained demonstrated the ability of EAH stem bark to ameliorate pancreatic β-cell dysfunction in alloxan-induced diabetic rats.

  17. Produção de serapilheira em sistema agroflorestal multiestratificado no Estado de Rondônia, Brasil Litter production in multistrata agroforestry system in Rondônia State, Brazil

    Directory of Open Access Journals (Sweden)

    Fernando Luíz de Oliveira Corrêa

    2006-12-01

    Full Text Available Objetivou-se com este trabalho avaliar a produção de serapilheira em diferentes coberturas frutíferas e florestais componentes de um sistema agroflorestal multiestratificado localizado no município de Ouro Preto do Oeste, RO, Brasil. As espécies avaliadas foram: mangueira (Mangifera indica L., fruta-pão (Artocarpus altilis (Parkinson Fosberg, cupuaçuzeiro (Theobroma grandiflorum Schum, abacateiro (Persea americana Mill., cacaueiro (Theobroma cacao L. sombreado com gliricídia (Gliricidia sepium (Jacq. Walp. e as espécies florestais, a bandarra (Schizolobium amazonicum Huber ex Ducke e a teca (Tectona grandis L.f.. Como referência foi utilizada uma área de vegetação natural (capoeira, de 8 anos de idade. A deposição anual de serapilheira entre o período de outubro de 2002 e setembro de 2003 foi de 13,38 t ha-1 para a vegetação natural, 4,02 t ha-1 para bandarra, 3,43 t ha-1 para gliricídia, 2,86 t ha-1para abacateiro, 2,54 t ha-1 para fruta-pão, 1,40 t ha-1 para o cupuaçuzeiro, 1,16 t ha-1 para o cacaueiro, 1,12 t ha-1 para mangueira e 1,07 t ha-1 para a teca. A vegetação natural, a bandarra e o cacaueiro apresentaram uma maior deposição no período seco (verão, enquanto que a fruta-pão, cupuaçuzeiro, mangueira, teca, gliricídia e abacateiro as maiores deposições foram no período chuvoso (inverno. Por outro lado a serapilheira acumulada sobre o solo foi de 14,61 t ha-1 na mangueira, 12,8 t ha-1 na bandarra, 12,73 t ha-1 na vegetação natural, 12,04 t ha-1, no abacateiro, 9,87 t ha-1 no cacaueiro/gliricídia, 9,88 t ha-1 no cupuaçuzeiro, 9,05 t ha-1 na fruta-pão e 7,39 t ha-1 na teca.One study was made to evaluate litter production in different fruitful coverings and forest components of a multistrata agroforestry system located in Ouro Preto do Oeste district (RO, Brazil. The studied species were: mango tree (Mangifera indica L., breadfruit tree (Artocarpus altilis (Parkinson Fosberg, cupuassu tree (Theobroma

  18. (Artocarpus heterophyllus) Lam.

    African Journals Online (AJOL)

    Awuor

    2015-04-22

    Apr 22, 2015 ... which lipid was extracted and the third group was boiled in water for. 2 h then lipid .... ratio of the detector response against concentration, plotted for each of the ..... and electrolyte leakage of Hayward kiwifruit during storage at.

  19. Thermodynamic properties of water sorption of jackfruit (Artocarpus heterophyllus Lam. as a function of moisture content Propriedades termodinâmicas de sorção de água da jaca (Artocarpus heterophyllus Lam. em função do teor de umidade

    Directory of Open Access Journals (Sweden)

    Ana Paula Prette

    2013-03-01

    Full Text Available The Jackfruit tree is one of the most significant trees in tropical home gardens and perhaps the most widespread and useful tree in the important genus Artocarpus. The fruit is susceptible to mechanical and biological damage in the mature state, and some people find the aroma of the fruit objectionable, particularly in confined spaces. The dehydration process could be an alternative for the exploitation of this product, and the relationship between moisture content and water activity provides useful information for its processing and storage. The aim of this study was to determine the thermodynamic properties of the water sorption of jackfruit (Artocarpus heterophyllus Lam. as a function of moisture content. Desorption isotherms of the different parts of the jackfruit (pulp, peduncle, mesocarp, peel, and seed were determined at four different temperatures (313.15, 323.15, 333.15, and 343.15 K in a water activity range of 0.02-0.753 using the static gravimetric method. Theoretical and empirical models were used to model the desorption isotherms. An analytical solution of the Clausius-Clapeyron equation was proposed to calculate the isosteric heat of sorption, the differential entropy, and Gibbs' free energy using the Guggenhein-Anderson-de Boer and Oswin models considering the effect of temperature on the hygroscopic equilibrium.A jaqueira é uma das árvores mais significativas nos quintais tropicais e, talvez, a árvore mais importante e útil do gênero Artocarpus. O fruto é suscetível a danos mecânicos e biológicos no estado maduro, e seu aroma é desagradável para algumas pessoas, quando em espaços fechados. O processo de desidratação pode ser uma alternativa para a exploração deste produto, e a relação entre a umidade e a atividade de água fornece informações úteis para seu processamento e armazenamento. O objetivo do trabalho foi determinar as propriedades termodinâmicas de sorção da água em frutos de jaca (Artocarpus

  20. Evaluation of the hypoglycemic and hypolipidemic effects of an ethylacetate fraction of Artocarpus heterophyllus (jak) leaves in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Chackrewarthy, S; Thabrew, M I; Weerasuriya, M K B; Jayasekera, S

    2010-07-01

    Aqueous extracts of mature leaves of Artocarpus heterophyllus (jak) are used by traditional medical practitioners in Sri Lanka and India for the treatment of diabetes. This study was conducted to investigate the hypoglycemic and hypolipidemic effects of an ethylacetate (EA) fraction of the mature leaves of A. heterophyllus in a streptozotocin (STZ) induced diabetic rat model. In normoglycemic rats, administration of a single dose (20 mg/kg) of the EA fraction resulted in a significant (P heterophyllus leaves daily for 5 weeks resulted in a significant lowering of serum glucose, cholesterol and triglyceride (TG) levels. Compared to control diabetic rats, the extract-treated rats had 39% less serum glucose, 23% lower serum total cholesterol and 40% lower serum TG levels and 11% higher body weight at the end of the fifth week. The percentage reductions in the serum parameters mediated by the test fraction were comparable with those produced by glibenclamide (0.6 mg/kg), the reference drug used in this study. It can be concluded that the EA fraction of A. heterophyllus leaves contains one or more hypoglycemic and hypolipidemic principles which have the potential to be developed further for the treatment of diabetes specifically associated with a hyperlipidemic state.

  1. Moracin C, A Phenolic Compound Isolated from Artocarpus heterophyllus, Suppresses Lipopolysaccharide-Activated Inflammatory Responses in Murine Raw264.7 Macrophages.

    Science.gov (United States)

    Yao, Xue; Wu, Dang; Dong, Ningning; Ouyang, Ping; Pu, Jiaqian; Hu, Qian; Wang, Jingyuan; Lu, Weiqiang; Huang, Jin

    2016-07-25

    Artocarpus heterophyllus, a popular tropical fruit commonly known as the jackfruit tree, is normally planted in subtropical or tropical areas. Since a variety of phytochemicals isolated from A. heterophyllus have been found to possess potently anti-inflammatory, antiviral and antimalarial activities, researchers have devoted much interest to its potential pharmaceutical value. However, the exact mechanism underlying its anti-inflammatory activity is not well characterized. In this study, seven natural products isolated from A. heterophyllus, including 25-Hydroxycycloart-23-en-3-one (HY), Artocarpin (AR), Dadahol A (DA), Morachalcone A (MA), Artoheterophyllin B (AB), Cycloheterophyllin (CY) and Moracin C (MC) were collected. Lipopolysaccharide (LPS)-stimulated inflammatory response in RAW264.7 macrophages were used in this study. Among these compounds, MC significantly inhibited LPS-activated reactive oxygen species (ROS) and nitric oxide (NO) release without marked cytotoxicity. Furthermore, MC effectively reduced LPS stimulated up-regulation of mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and serval pro-inflammatory cytokines (interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α)). Mechanistic studies revealed that the anti-inflammatory effect of MC was associated with the activation of the mitogen activated protein kinases (MAPKs) (including p38, ERK and JNK) and nuclear factor-κB (NF-κB) pathways, especially reducing the nuclear translocation of NF-κB p65 subunit as revealed by nuclear separation experiment and confocal microscopy.

  2. Evaluation of antioxidant potential of Artocarpus heterophyllus L. J33 variety fruit waste from different extraction methods and identification of phenolic constituents by LCMS.

    Science.gov (United States)

    Daud, Mohd Nazrul Hisham; Fatanah, Dian Nashiela; Abdullah, Noriham; Ahmad, Rohaya

    2017-10-01

    Artocarpus heterophyllus J33 (AhJ33) fruit is a popular and valuable jackfruit variety in Malaysia. For export, the pulp has to be separated from the skin which is usually discarded. Hence, the conversion of the fruit waste to food products with economic value needs to be explored utilizing the waste to wealth concept. This paper reports the evaluation of antioxidant potential of AhJ33 fruit waste (rind and rachis) extracts from three different extraction methods (maceration, percolation and Soxhlet). The antioxidant potential was assessed by DPPH radical scavenging, FRAP and β-carotene bleaching assays. The total phenolic and total flavonoid contents were estimated by TPC and the TFC assays. For both rind and rachis, the maceration technique yielded extracts with the strongest antioxidant activities which correlated with the highest TPC and TFC values. TOF LCMS analyses identified two phenolic acids as the major constituents responsible for the antioxidant activity of the active extracts. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Inhibitor y effect on key enzymes relevant to acute type-2 diabetes and antioxidative activity of ethanolic extract of Artocarpus heterophyllus stem bark

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    Basiru Olaitan Ajiboye

    2016-09-01

    Full Text Available Objective: To investigate the in vitro antioxidant activity of ethanolic extract of Artocarpus heterophyllus (A. heterophyllus stem bark and its inhibitory effect on a-amylase and a-glucosidase. Methods: The A. heterophyllus stem bark was extracted using methanol and tested for antioxidative activity. Results: The results revealed that the ethanolic extract has polyphenolics and free radical scavenging compounds which were significantly higher (P < 0.05 than their respective standard, at concentration dependent manner. The ethanolic extract of A. heterophyllus stem bark was observed to show inhibitory activities on a-amylase and a-glucosidase with IC50 of (4.18 ± 0.01 and (3.53 ± 0.03 mg/mL, respectively. The Lineweaver-Burk plot revealed that ethanolic extract of A. heterophyllus stem bark exhibited non-competitive inhibition for a-amylase and uncompetitive inhibition for a-glucosidase activities. Also, gas chromatography–mass spectrometry showed the presence of different bioactive compounds in extract. Conclusions: Therefore, it can be inferred from this study that ethanolic extract of A. heterophyllus stem bark may be useful in the management of diabetes mellitus probably due to bioactive compounds observed in the extract.

  4. A synergistic effect of artocarpanone from Artocarpus heterophyllus L. (Moraceae) on the antibacterial activity of selected antibiotics and cell membrane permeability.

    Science.gov (United States)

    Septama, Abdi Wira; Xiao, Jianbo; Panichayupakaranant, Pharkphoom

    2017-01-01

    Artocarpanone isolated from Artocarpus heterophyllus L. (Moraceae) exhibits antibacterial activity. The present study investigated synergistic activity between artocarpanone and tetracycline, ampicillin, and norfloxacin, respectively, against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa , and Escherichia coli . A broth microdilution method was used for evaluating antibacterial susceptibility. Synergistic effects were identified using a checkerboard method, and a bacterial cell membrane disruption was investigated by assay of released 260 nm absorbing materials following bacteriolysis. Artocarpanone exhibited weak antibacterial activity against MRSA and P. aeruginosa with minimum inhibitory concentrations values of 125 and 500 μg/mL, respectively. However, the compound showed strong antibacterial activity against E. coli (7.8 μg/mL). The interaction between artocarpanone and all tested antibiotics revealed indifference and additive effects against P. aeruginosa and E. coli (fractional inhibitory concentration index [FICI] values of 0.75-1.25). The combination of artocarpanone (31.2 μg/mL) and norfloxacin (3.9 μg/mL) resulted in synergistic antibacterial activity against MRSA, with an FICI of 0.28, while the interaction between artocarpanone and tetracycline, and ampicillin showed an additive effect, with an FICI value of 0.5. A time-kill assay also indicated that artocarpanone had a synergistic effect on the antibacterial activity of norfloxacin. In addition, the combination of artocarpanone and norfloxacin altered the membrane permeability of MRSA. These findings suggest that artocarpanone may be used to enhance the antibacterial activity of norfloxacin against MRSA.

  5. Norartocarpetin from a folk medicine Artocarpus communis plays a melanogenesis inhibitor without cytotoxicity in B16F10 cell and skin irritation in mice.

    Science.gov (United States)

    Ko, Horng-Huey; Tsai, Yi-Ting; Yen, Ming-Hong; Lin, Chun-Ching; Liang, Chan-Jung; Yang, Tsung-Han; Lee, Chiang-Wen; Yen, Feng-Lin

    2013-12-10

    Many natural products used in preventive medicine have also been developed as cosmeceutical ingredients in skin care products, such as Scutellaria baicalensis and Gardenia jasminoides. Norartocarpetin is one of the antioxidant and antityrosinase activity compound in Artocarpus communis; however, the cytotoxicity, skin irritation and antimelanogenesis mechanisms of norartocarpetin have not been investigated yet. In the present study, cell viability in vitro and skin irritation in vivo are used to determine the safety of norartocarpetin. The melanogenesis inhibition of norartocarpetin was determined by cellular melanin content and tyrosinase in B16F10 melanoma cell. Moreover, we examined the related-melanogenesis protein by western blot analysis for elucidating the antimelanogenesis mechanism of norartocarpin. The result of the present study demonstrated that norartocarpetin not only present non-cytotoxic in B16F10 and human fibroblast cells but also non-skin irritation in mice. Moreover, our result also first found that norartocarpetin downregulated phospho-cAMP response element-binding (phospho-CREB) and microphthalmia-associated transcription factor (MITF) expression, which in turn decreased both synthesis of tyrosinases (TRP-1 and TRP-2) and cellular melanin content. This process is dependent on norartocarpetin phosphorylation by mitogen-activated protein kinases such as phospho-JNK and phospho-p38, and it results in decreased melanogenesis. The present study suggests that norartocarpetin could be used as a whitening agent in medicine and/or cosmetic industry and need further clinical study.

  6. Bluish green emitting carbon quantum dots synthesized from jackfruit (Artocarpus heterophyllus) and its sensing applications of Hg (II) and Cr (VI) ions

    Science.gov (United States)

    Rajendran, Kalimuthu; Rajendiran, Nagappan

    2018-02-01

    A simple, economical, and green method for the preparation of water soluble, high fluorescent carbon quantum dots (CQDs) has been prepared via hydrothermal process using jackfruit (Artocarpus heterophyllus) as a carbon source. The optical properties of synthesized CQDs were characterized by UV- visible and fluorescence spectroscopy. Fourier transform infrared spectroscopy (FT-IR), x-ray Diffraction (XRD) and high resolution transmission electron microscopy (HR-TEM) techniques were used to study the composition and size of the CQDs. The prepared CQDs were spherical in shape with an average size of 2.5 nm along with uniform distribution and showed bright bluish green emission properties, without any further surface modification. The prepared CQDs were exhibit high stability at neutral pH and showed high photo-stability under UV light irradiation at 365 nm. The obtained CQDs were effectively utilized as fluorescent probe for highly selective and sensitive detection of Hg2+ and Cr6+ ions in environmental samples with a limit of detection of about 8 and 10 nM respectively.

  7. In vitro anti-acne activity of phytoactives from the stem bark of Artocarpus hirsutus Lam. and characterisation of pyranocycloartobiloxanthone A as a mixture of two anomers.

    Science.gov (United States)

    Nayak, Mahadeva; Nagarajan, A; Majeed, Muhammed; Nagabhushanam, Kalyanam; Choudhury, Ambar K

    2017-08-18

    This study is aimed to isolate the phytoactives from the stem bark of Artocarpus hirsutus and evaluate their in vitro anti-acne activity. The ethanolic stem bark extract of A. hirsutus provided two major phytoactive constituents: (i) pyranocycloartobiloxanthone A, (1) and (ii) Artonine E, (2) whose structures were determined by NMR and MS spectroscopic analysis. The present study is the first to report compound 1 as a mixture of two anomers (α and β), approximately 70:30 ratio. Both compounds 1 and 2 were isolated for the first time from this plant. In vitro anti-acne activity of compounds 1 and 2 were evaluated by agar well diffusion method and the minimum inhibition was determined by broth micro dilution method. The result of anti-microbial activity (MIC = 2.0 μg/mL each) is comparable to antibiotic, Clindamycin (MIC = 0.03 μg/mL) and clearly demonstrate their potential as anti-acne agents.

  8. Avaliação do uso de pomada à base de sementes de jaqueira (Artocarpus heterophyllus Lam na terapêutica tópica de feridas

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    R. N.L. Vitorino Filho

    2009-07-01

    Full Text Available As propriedades cicatrizantes da jaqueira (Artocarpus heterophyllus Lam são atribuídas a uma proteína encontrada na semente de sua fruta que, em casos de queimaduras de pele, proporciona a regeneração do tecido danificado em vez da substituição por tecido conjuntivo fibroso. Objetivando avaliar macro e microscopicamente o efeito da pomada formulada com o pó obtido a partir de sementes de jaca na cicatrização, por segunda intenção, de feridas cutâneas em camundongos, limpas e infectadas experimentalmente por Staphylococcus aureus, realizou-se o estudo em 96 camundongos nos quais foram feitas feridas experimentais na região dorso-lateral. Cada grupo, com 12 animais, recebeu um tipo de tratamento. O grupo GI serviu como controle para feridas limpas, o grupo GII foi o controle para feridas infectadas e os demais foram tratados topicamente com pomada à base de semente de jaca em diferentes esquemas de administração. Os animais foram avaliados diariamente e sacrificados cinco, 10 e 13 dias após a cirurgia, sendo removido o tecido cicatricial e circunjacente para avaliação histológica. Os resultados mostraram que não houve diferença estatisticamente significativa entre os tratamentos. Palavras-chave: camundongos; cicatrização; feridas; jaca; Artocarpus heterophyllus Lam; lectina.

  9. Antioxidant activity of Artocarpus heterophyllus Lam. (Jack Fruit) leaf extracts: remarkable attenuations of hyperglycemia and hyperlipidemia in streptozotocin-diabetic rats.

    Science.gov (United States)

    Omar, Haidy S; El-Beshbishy, Hesham A; Moussa, Ziad; Taha, Kamilia F; Singab, Abdel Nasser B

    2011-04-05

    The present study examines the antioxidative, hypoglycemic, and hypolipidemic activities of Artocarpus heterophyllus (jack fruit) leaf extracts (JFEs). The 70% ethanol (JFEE), n-butanol (JFBE), water (JFWE), chloroform (JFCE), and ethyl acetate (JFEAE) extracts were obtained. Both JFEE and JFBE markedly scavenge diphenylpicrylhydrazyl radical and chelate Fe+2 in vitro. A compound was isolated from JFBE and identified using 1D and 2D 1H- and 13C-NMR. The administration of JFEE or JFBE to streptozotocin (STZ)-diabetic rats significantly reduced fasting blood glucose (FBG) from 200 to 56 and 79 mg%, respectively; elevated insulin from 10.8 to 19.5 and 15.1 µU/ml, respectively; decreased lipid peroxides from 7.3 to 5.4 and 5.9 nmol/ml, respectively; decreased %glycosylated hemoglobin A1C (%HbA1C) from 6.8 to 4.5 and 5.0%, respectively; and increased total protein content from 2.5 to 6.3 and 5.7 mg%, respectively. Triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), VLDL-C, and LDL/HDL ratio significantly declined by -37, -19, -23, -37, and -39%, respectively, in the case of JFEE; and by -31, -14, -17, -31, and -25%, respectively, in the case of JFBE; as compared to diabetic rats. HDL-C increased by +37% (JFEE) and by +11% (JFBE). Both JFEE and JFBE have shown appreciable results in decreasing FBG, lipid peroxides, %HbA1C, TC, LDL-C, and TG levels, and increasing insulin, HDL-C, and protein content. The spectrometric analysis confirmed that the flavonoid isolated from JFBE was isoquercitrin. We can conclude from this study that JFEE and JFBE exert hypoglycemic and hypolipidemic effects in STZ-diabetic rats through an antioxidative pathway that might be referred to their flavonoid contents.

  10. Abundance of Jackfruit ( Artocarpus heterophyllus) Affects Group Characteristics and Use of Space by Golden-Headed Lion Tamarins ( Leontopithecus chrysomelas) in Cabruca Agroforest

    Science.gov (United States)

    Oliveira, Leonardo C.; Neves, Leonardo G.; Raboy, Becky E.; Dietz, James M.

    2011-08-01

    Cabruca is an agroforest of cacao trees shaded by native forest trees. It is the predominant vegetation type throughout eastern part of the range of the golden-headed lion tamarins, Leontopithecus chrysomelas, an endangered primate endemic to Atlantic Forest. Understanding how lion tamarins use this agroforest is a conservation priority. To address this question, we documented the diet, home range size, group sizes and composition, density, number of litters and body condition of lion tamarins living in cabruca, and other habitats. Jackfruit, Artocarpus heterophyllus, was the most used species used by lion tamarins in cabruca and was widely available and used throughout the year. In cabruca, home range size was the smallest (22-28 ha) and density of lion tamarins was the highest (1.7 ind/ha) reported for the species. Group size averaged 7.4 individuals and was not significantly different among the vegetation types. In cabruca, groups produced one or two litters a year, and all litters were twins. Adult males in cabruca were significantly heavier than males in primary forest. Our study is the first to demonstrate that breeding groups of golden-headed lion tamarins can survive and reproduce entirely within cabruca agroforest. Jackfruit proved to be a keystone resource for lion tamarins in cabruca, and bromeliads were important as an animal prey foraging microhabitat. In cases where cabruca contains concentrated resources, such as jackfruit and bromeliads, lion tamarins may not only survive and reproduce but may fare better than in other forest types, at least for body condition and reproduction.

  11. Moracin C, A Phenolic Compound Isolated from Artocarpus heterophyllus, Suppresses Lipopolysaccharide-Activated Inflammatory Responses in Murine Raw264.7 Macrophages

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    Xue Yao

    2016-07-01

    Full Text Available Artocarpus heterophyllus, a popular tropical fruit commonly known as the jackfruit tree, is normally planted in subtropical or tropical areas. Since a variety of phytochemicals isolated from A. heterophyllus have been found to possess potently anti-inflammatory, antiviral and antimalarial activities, researchers have devoted much interest to its potential pharmaceutical value. However, the exact mechanism underlying its anti-inflammatory activity is not well characterized. In this study, seven natural products isolated from A. heterophyllus, including 25-Hydroxycycloart-23-en-3-one (HY, Artocarpin (AR, Dadahol A (DA, Morachalcone A (MA, Artoheterophyllin B (AB, Cycloheterophyllin (CY and Moracin C (MC were collected. Lipopolysaccharide (LPS-stimulated inflammatory response in RAW264.7 macrophages were used in this study. Among these compounds, MC significantly inhibited LPS-activated reactive oxygen species (ROS and nitric oxide (NO release without marked cytotoxicity. Furthermore, MC effectively reduced LPS stimulated up-regulation of mRNA and protein expression of inducible nitric oxide synthase (iNOS, cyclooxygenase-2 (COX-2, and serval pro-inflammatory cytokines (interleukin-1β (IL-1β, interleukin-6 (IL-6 and tumor necrosis factor α (TNF-α. Mechanistic studies revealed that the anti-inflammatory effect of MC was associated with the activation of the mitogen activated protein kinases (MAPKs (including p38, ERK and JNK and nuclear factor-κB (NF-κB pathways, especially reducing the nuclear translocation of NF-κB p65 subunit as revealed by nuclear separation experiment and confocal microscopy.

  12. A Synergistic effect of artocarpanone from Artocarpus heterophyllus Lam. (Moraceae on the antibacterial activity of some antibiotics and their effect on membrane permeability

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    Abdi Wira Septama

    2017-06-01

    Full Text Available Aim/backgrounds: Artocarpanone isolated from Artocarpus heterophyllus Lam. (Moraceae possesses antibacterial activity. The present study investigated any interaction between artocarpanone and some antibiotics including tetracycline, ampicillin and norfloxacin against Methicillin-resistant Staphylococcus aureus (MRSA, Pseudomonas aeruginosa and Escherichia coli, as well as determining any disruptive effect on bacterial membranes. Materials and methods: A broth microdilution method was used for the susceptibility assay. Any synergistic effect was determined using a checerboard method, and any membrane disruption effect was investigated using a bacteriolysis assay and a measurement of the released 260 nm absorbing materials. Results and discussion: Artocarpanone exhibited weak antibacterial activities against MRSA and P. aeruginosa with MIC values of 125 and 500 µg/mL, respectively. However, it showed the strong antibacterial activity against E. coli (7.8 µg/mL. The interaction between artcarpanone with all tested antibiotics against P. aeruginosa and E. coli only revealed indifference and additive effects (FICI values of 0.75-1.25. The interaction between artocarpanone (31.2 µg/mL and norfloxacin (3.9 µg/mL exhibited a synergistic antibacterial activity against MRSA, with a fractional inhibitory concentration index (FICI of 0.28, while the interaction between artocarpanone and tetracycline, and ampicillin showed an additive effect, with an FICI value of 0.5. A time kill assay also indicated that artocarpanone had a synergistic effect on the antibacterial activity of norfloxacin. In addition, a combination of artocarpanone and norfloxacin altered the membrane permeability of MRSA. Conclusion: These findings suggested that artocarpanone may be considered as an adjuvant to enhance the antibacterial activity of norfloxacin against MRSA. [J Complement Med Res 2017; 6(2.000: 186-191

  13. Antioxidant Activity of Artocarpus heterophyllus Lam. (Jack Fruit Leaf Extracts: Remarkable Attenuations of Hyperglycemia and Hyperlipidemia in Streptozotocin-Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Haidy S. Omar

    2011-01-01

    Full Text Available The present study examines the antioxidative, hypoglycemic, and hypolipidemic activities of Artocarpus heterophyllus (jack fruit leaf extracts (JFEs. The 70% ethanol (JFEE, n-butanol (JFBE, water (JFWE, chloroform (JFCE, and ethyl acetate (JFEAE extracts were obtained. Both JFEE and JFBE markedly scavenge diphenylpicrylhydrazyl radical and chelate Fe+2in vitro. A compound was isolated from JFBE and identified using 1D and 2D 1H- and 13C-NMR. The administration of JFEE or JFBE to streptozotocin (STZ-diabetic rats significantly reduced fasting blood glucose (FBG from 200 to 56 and 79 mg%, respectively; elevated insulin from 10.8 to 19.5 and 15.1 μU/ml, respectively; decreased lipid peroxides from 7.3 to 5.4 and 5.91 nmol/ml, respectively; decreased %glycosylated hemoglobin A1C (%HbA1C from 6.8 to 4.5 and 5.0%, respectively; and increased total protein content from 2.5 to 6.3 and 5.7 mg%, respectively. Triglycerides (TG, total cholesterol (TC, low-density lipoprotein cholesterol (LDL-C, VLDL-C, and LDL/HDL ratio significantly declined by -37, -19, -23, -37, and -39%, respectively, in the case of JFEE; and by -31, -14, -17, -31, and -25%, respectively, in the case of JFBE; as compared to diabetic rats. HDL-C increased by +37% (JFEE and by +11% (JFBE. Both JFEE and JFBE have shown appreciable results in decreasing FBG, lipid peroxides, %HbA1C, TC, LDL-C, and TG levels, and increasing insulin, HDL-C, and protein content. The spectrometric analysis confirmed that the flavonoid isolated from JFBE was isoquercitrin. We can conclude from this study that JFEE and JFBE exert hypoglycemic and hypolipidemic effects in STZ-diabetic rats through an antioxidative pathway that might be referred to their flavonoid contents.

  14. Suivi du Gecko Phelsuma serraticauda (Squamata : Gekkonidae ...

    African Journals Online (AJOL)

    La plupart des observations des individus sur les plantes sont sur le cocotier Cocos nucifera (84,3 % ) et le fruit de pain Artocarpus altilis (4,9 % ), avec 8,3 % sur le mur d'une maison en bois. Le nombre de P. serraticauda varie entre un et cinq par biotope avec une moyenne de 2,6 ± 0,2 pour l'ensemble des plantes et de 1 ...

  15. About rats and jackfruit trees: modeling the carrying capacity of a Brazilian Atlantic Forest spiny-rat Trinomys dimidiatus (Günther, 1877) - Rodentia, Echimyidae - population with varying jackfruit tree (Artocarpus heterophyllus L.) abundances.

    Science.gov (United States)

    Mello, J H F; Moulton, T P; Raíces, D S L; Bergallo, H G

    2015-01-01

    We carried out a six-year study aimed at evaluating if and how a Brazilian Atlantic Forest small mammal community responded to the presence of the invasive exotic species Artocarpus heterophyllus, the jackfruit tree. In the surroundings of Vila Dois Rios, Ilha Grande, RJ, 18 grids were established, 10 where the jackfruit tree was present and eight were it was absent. Previous results indicated that the composition and abundance of this small mammal community were altered by the presence and density of A. heterophyllus. One observed effect was the increased population size of the spiny-rat Trinomys dimidiatus within the grids where the jackfruit trees were present. Therefore we decided to create a mathematical model for this species, based on the Verhulst-Pearl logistic equation. Our objectives were i) to calculate the carrying capacity K based on real data of the involved species and the environment; ii) propose and evaluate a mathematical model to estimate the population size of T. dimidiatus based on the monthly seed production of jackfruit tree, Artocarpus heterophyllus and iii) determinate the minimum jackfruit tree seed production to maintain at least two T. dimidiatus individuals in one study grid. Our results indicated that the predicted values by the model for the carrying capacity K were significantly correlated with real data. The best fit was found considering 20~35% energy transfer efficiency between trophic levels. Within the scope of assumed premises, our model showed itself to be an adequate simulator for Trinomys dimidiatus populations where the invasive jackfruit tree is present.

  16. About rats and jackfruit trees: modeling the carrying capacity of a Brazilian Atlantic Forest spiny-rat Trinomys dimidiatus (Günther, 1877 – Rodentia, Echimyidae – population with varying jackfruit tree (Artocarpus heterophyllus L. abundances

    Directory of Open Access Journals (Sweden)

    JHF Mello

    Full Text Available We carried out a six-year study aimed at evaluating if and how a Brazilian Atlantic Forest small mammal community responded to the presence of the invasive exotic species Artocarpus heterophyllus, the jackfruit tree. In the surroundings of Vila Dois Rios, Ilha Grande, RJ, 18 grids were established, 10 where the jackfruit tree was present and eight were it was absent. Previous results indicated that the composition and abundance of this small mammal community were altered by the presence and density of A. heterophyllus. One observed effect was the increased population size of the spiny-rat Trinomys dimidiatus within the grids where the jackfruit trees were present. Therefore we decided to create a mathematical model for this species, based on the Verhulst-Pearl logistic equation. Our objectives were i to calculate the carrying capacity K based on real data of the involved species and the environment; ii propose and evaluate a mathematical model to estimate the population size of T. dimidiatus based on the monthly seed production of jackfruit tree, Artocarpus heterophyllus and iii determinate the minimum jackfruit tree seed production to maintain at least two T. dimidiatus individuals in one study grid. Our results indicated that the predicted values by the model for the carrying capacity K were significantly correlated with real data. The best fit was found considering 20~35% energy transfer efficiency between trophic levels. Within the scope of assumed premises, our model showed itself to be an adequate simulator for Trinomys dimidiatus populations where the invasive jackfruit tree is present.

  17. Efeitos da inclusão do farelo de sementes de jaqueira Artocarpus heterophyllus Lam. na ração sobre a produção, pigmentação da gema e umidade fecal em codornas Effect of the addition of jackfruit (Artocarpus heterophyllus Lam. seeds bran in dietary on the egg production, yolk pigmentation and dropping humidity in japanese quails

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    José Humberto Vilar da Silva

    2007-04-01

    Full Text Available Um experimento foi conduzido com o objetivo de avaliar o efeito de níveis crescentes do farelo da semente de jaqueira (Artocarpus heterophyllus Lam. na ração sobre a produção e pigmentação da gema dos ovos e umidade das excretas em 180 codornas japonesas (Coturnix coturnix japonica. Aos 42 dias de idade, as aves foram distribuídas em delineamento inteiramente casualizado e alimentadas com rações contendo níveis de 0; 2; 4; 6; 8 e 10% do farelo de semente de jaqueira (FSJ. Foram avaliados o consumo de ração, produção de ovos, peso dos ovos, massa de ovos, conversão por massa e por dúzia de ovos, além do peso e da porcentagem de gema, clara, casca, pigmentação da gema dos ovos e umidade das excretas. O aumento do FSJ na ração piorou linearmente (PAn experiment was carried out to evaluate the effect of increase levels of jackfruit (Artocarpus heterophyllus Lam. seeds bran in diet of japanese quails on egg production, yolk pigmentation and dropping humidity . A total of 180 japanese quails (Coturnix coturnix japonica with 42 days oold were distributed in a completely randomized design and fed diets with differents levels of 0; 2; 4; 6; 8 and 10% of jackfruit seeds bran (JSB. The variables feed intake, egg production, egg weigh, egg mass, feed egg mass conversion and feed egg dozen conversion as well as the yolk weight and yolk percentage, egg white, egg shell and egg yolk pigmentation. The data shown that increasing JSB in the diets reduced the egg production and higher feed egg dozen conversion lineally (P<0,05. Therefore the egg yolk pigmentation improved up to 7.44% of JSB in the diet and the dropping humidity was higher up to 4.9% of JSB.The presence of antitrypsin factor is a possible explanation for lower performance on the japanese quails fed diets with increase of JSB fresh. In order to solve this problem e, it is recommended to expose the seeds to a thermal treatment, otherwise the jackfruit seed fresh should not be

  18. Maesopsin 4-O-beta-D-glucoside, a natural compound isolated from the leaves of Artocarpus tonkinensis, inhibits proliferation and up-regulates HMOX1, SRXN1 and BCAS3 in acute myeloid leukemia.

    Science.gov (United States)

    Pozzesi, N; Pierangeli, S; Vacca, C; Falchi, L; Pettorossi, V; Martelli, M P; Thuy, T T; Ninh, P T; Liberati, A M; Riccardi, C; Sung, T V; Delfino, D V

    2011-06-01

    The leaves of Artocarpus tonkinensis are used in Vietnamese traditional medicine for treatment of arthritis, and the compound maesopsin 4-O-β-D-glucoside (TAT-2), isolated from them, inhibits the proliferation of activated T cells. Our goal was to test the anti-proliferative activity of TAT-2 on the T-cell leukemia, Jurkat, and on the acute myeloid leukemia, OCI-AML. TAT-2 inhibited the growth of OCI-AML (and additional acute myeloid leukemia cells) but not Jurkat cells. Growth inhibition was shown to be due to inhibition of proliferation rather than increase in cell death. Analysis of cytokine release showed that TAT-2 stimulated the release of TGF-β, yet TGF-β neutralization did not reverse the maesopsin-dependent effect. Gene expression profiling determined that maesopsin modulated 19 identifiable genes. Transcription factor CP2 was the gene most significantly modulated. Real-time PCR validated that up-regulation of sulphiredoxin 1 homolog (SRXN1), hemeoxygenase 1 (HMOX1), and breast carcinoma amplified sequence 3 (BCAS3) were consistently modulated.

  19. Jackfruit (Artocarpus heterophyllus Lam.) peel: A better source of antioxidants and a-glucosidase inhibitors than pulp, flake and seed, and phytochemical profile by HPLC-QTOF-MS/MS.

    Science.gov (United States)

    Zhang, Lu; Tu, Zong-Cai; Xie, Xing; Wang, Hui; Wang, Hao; Wang, Zhen-Xing; Sha, Xiao-Mei; Lu, Yu

    2017-11-01

    Jackfruit (Artocarpus heterophyllus Lam.) peel is an underutilized by-product in both, the production and processing of jackfruit. This research compared the antioxidant and hypoglycemic potential of jackfruit peel with jackfruit pulp, flake and seed for the first time. The phytochemical profile of peel extract was characterized with HPLC-QTOF-MS/MS. Results revealed that peel extract exhibited the highest total phenolic and total flavonoid content, and the phenolics was 4.65, 4.12 and 4.95 times higher than that of pulp, flake and seed extract, respectively. The strongest DPPH and ABTS + scavenging ability, α-glucosidase inhibition were also found in peel extract, and the α-glucosidase inhibition was about 11.8-fold of that of acarbose. The HPLC-QTOF-MS/MS analysis led to the tentative identification of 53 compounds, prenylflavonoids, hydroxycinnamic acids and glycosides are the predominant bioactive compounds. Above results reveal promising potential of jackfruit peel as a new source of natural antioxidants and hypoglycemic agents. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Nutritional Composition of the Flour of African Breadfruit ( Treculia ...

    African Journals Online (AJOL)

    The PUFA/SFA ratio indicated high level of essential FAs. Essential PUFA standard index (EPSI) (1.17), MUFA/SFA (0.564), LA/ALA (69.0), n-6/n-3 (103) and EPA/DHA (1.00) were observed. These values showed that quality of PUFA was high. The most prominent phospholipid and phytosterol were phosphatidylcholine ...

  1. THE EFFECT OF (Treculia Africana) AFRICAN BREADFRUIT ON ...

    African Journals Online (AJOL)

    DJFLEX

    equilibrium between the intracellular compartment. Potassium is vital to homeostasis. The major force maintaining the difference in cation concentration between the intracellular fluid and the extra cellular fluid is the activity of sodium potassium pump intergral to all cell membrane (Field et al 2006) this may explain the.

  2. Design of Breadfruit Shelling Machine | Nwigbo | African Research ...

    African Journals Online (AJOL)

    In the engineering design of this machine, the action zone consisted essentially of two rollers; one adjustable and the other rotating. A separating unit that cleans the seed while pneumatically separating it from the shell was incorporated. The design of this separating unit was such that practically 80% cleaning was achieved ...

  3. Socio-economic contribution of African breadfruit ( Treculia africana ...

    African Journals Online (AJOL)

    The Non-Timber Forest Products (NTFPs) the conserve are gathered for household consumption and commercial uses; they have formed an inherent part of rural ... the level of income generation, processing, distribution of sales as well as the importance of Treculia africana to food security in Southeastern, Nigeria in 2015.

  4. In Vivo Antiplasmodial Potentials of the Combinations of Four Nigerian Antimalarial Plants

    Directory of Open Access Journals (Sweden)

    Adeleke Clement Adebajo

    2014-08-01

    Full Text Available Various combinations of Nauclea latifolia root, Artocarpus altilis stem bark, Murraya koenigii leaf and Enantia chlorantha stem bark used in African ethnomedicine as decoctions for malaria and fevers, and combinations with standard drugs, were investigated for antiplasmodial activities using Plasmodium berghei berghei-infected mice. The respective prophylactic and curative ED50 values of 189.4 and 174.5 mg/kg for N. latifolia and chemosuppressive ED50 value of 227.2 mg/kg for A. altilis showed that they were the best antimalarial herbal drugs. A 1.6-fold increase of the survival time given by the negative control was elicited by M. koenigii, thereby confirming its curative activity. Pyrimethamine with an ED50 of 0.5 ± 0.1 mg/kg for the prophylactic, and chloroquine with ED50 = 2.2 ± 0.1 and 2.2 ± 0.0 mg/kg for the chemosuppressive and curative tests, respectively, were significantly (p < 0.05 more active. Co-administrations of N. latifolia with the standard drugs significantly reduced their prophylactic, chemosuppressive and curative actions, possibly increasing the parasites’ resistance. Binary combinations of N. latifolia or M. koenigii with any of the other plants significantly increased the prophylactic and suppressive activities of their individual plants, respectively. Also, E. chlorantha with A. altilis or N. latifolia enhanced their respective prophylactic or curative activities, making these combinations most beneficial against malaria infections. Combinations of three and four extracts gave varied activities. Hence, the results justified the combinations of ethnomedicinal plants in antimalarial herbal remedies and showed the importance of the three in vivo models in establishing antimalarial activity.

  5. Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study

    Directory of Open Access Journals (Sweden)

    Carla eOliveira

    2014-08-01

    Full Text Available Frutalin is a homotetrameric partly-glycosylated alpha-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that batch-to-batch variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine.

  6. Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study

    Science.gov (United States)

    Oliveira, Carla; Teixeira, José A.; Domingues, Lucília

    2014-01-01

    Frutalin is a homotetrameric partly glycosylated α-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine. PMID:25152749

  7. A Disposable Sensor For Assessing Artocarpus heterophyllus L. (Jackfruit Maturity

    Directory of Open Access Journals (Sweden)

    Chang Chew Cheen

    2003-12-01

    Full Text Available The purpose of this work was an attempt to monitor the ripeness process and to investigate the different maturity stages of jackfruit by chemometric treatment of the data obtained from the disposable sensor. Response of the sensor strip fabricated using screenprinting technology was analyzed using Principal Component Analysis (PCA and the classification model constructed by means of Canonical Discriminant Analysis (CDA enable unknown maturity stages of jackfruit to be identified. Results generated from the combination of the two classification principles show the capability and the performance of the sensor strip towards jackfruit analysis.

  8. Phytochemical Studies and Antioxidant Activities of Artocarpus scortechinii King

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    Norzafneza Mohd Arriffin

    2017-05-01

    Full Text Available Six known compounds identified as 4',5-dihydroxy-6,7-(2,2-dimethylpyrano-2'-methoxy-8-γ,γ-dimethylallyflavone (1, cudraflavone A (2, artocarpin(3, cycloartobiloxanthone (4, artonin E (5 and oxyresveratrol (6 were isolated from the stem barks of Artocapus scortechinii King. Another four compounds namely macakurzin C (7, flemichapparin A (8, luteolin (9 and apigenin (10 were isolated from the leaves. Structures of all pure compounds were elucidated spectroscopically using 1D NMR ( 1H, 13C, DEPT, 2D NMR (COSY, HMQC, HMBC, MS and FTIR, as well as by comparison with literature data. All isolated compounds were evaluated for their antioxidant capacities using 2,2-diphenyl-1-picrylhydrazyl (DPPH, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS and ferric reducing antioxidant potential (FRAP assays. Luteolin (9 showed the best ability to act as DPPH free radical scavenger with IC 50 value of 9.51 µM. Luteolin (9 also exhibited the strongest scavenger and potent ferric ion reducer compared to other isolated compounds in the ABTS and FRAP assays with IC 50 value of 124.4µM and 4.07 ± 0.180 mM FRAP equivalent respectively.

  9. Physicochemical Properties of Dietary Fibers from Artocarpus camansi Fruit

    Science.gov (United States)

    Suryanti, V.; Kusumaningsih, T.; Rumingtyas, Y. S.

    2017-04-01

    The objective of this work was to investigate the dietary fiber (DF) contents of Artocorpus camansi (breadnut) fruit and examine their physicochemical properties, such as water-holding capacity (WAC), oil-holding capacity (OHC) and water absorption capacity (WAC). This fruit flour contained of both water soluble fibers (SDF), such as pectin (1.95%) and gum (0.4%), and water insoluble fibers (IDF) (89.25%). The IDF content of this fruit was significantly high in respect to other DF sources. The WHC, OHC and WAC of IDF were 4.10, 2.60 and 4.0%, respectively. Moreover, the WHC, OHC and WHC of total dietary fibers (TDF) were 4.2, 4.3 and 4.6%, respectively. The results showed that the DF of fruit flour had good physicochemical properties. The findings suggested that there is a potential application of A. camansi of fruit as functional ingredients in the food industry.

  10. In vitro Antimicrobial and Antibiofilm Activity of Artocarpus Lakoocha ...

    African Journals Online (AJOL)

    Representative strains of oral pathogens (Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 33478, Enterococcus faecalis ATCC 19433, Lactobacillus fermentum ATCC 14931, Lactobacillus salivarius ATCC 11741, Aggregatibacter actinomycetemcomitans ATCC 33384, Porphyromonas gingivalis ATCC ...

  11. Osmo-convective dehydration kinetics of jackfruit (Artocarpus heterophyllus

    Directory of Open Access Journals (Sweden)

    Pragati Kaushal

    2016-06-01

    Full Text Available Osmotic dehydration is a process in which partial water is removed by immersion of water containing cellular solid in a concentrated aqueous solution of high osmotic media for a specific time and temperature. Preliminary trials were planned for finalizing the concentration of osmolyte (salt solution: 5%, 10%, 15% and 20%. The osmotically pre-treated samples were dried at 50 °C which were examined using sensory parameters. On the basis of sensory parameters, 15% salt solution concentration was considered best. The osmotically pre-treated jackfruit samples of 15% salt solution were convectively dehydrated in a tray dryer at air temperatures of 50, 60 and 70 °C at constant velocity of 1.5 m/s air flow in perforated trays. Results indicated that drying took place in falling rate period. The sample dried at 60 °C was found better in color as compared to samples at 50 and 70 °C. Mathematical models were fitted to the experimental data and the performance of these models was evaluated by comparing the coefficient of determination (R2, Root mean square error (RMSE, reduced chi-square (χ2, percent mean relative deviation modulus (E% between observed and predicted moisture ratio. The best model was chosen as one with the highest coefficient of correlation (R2; and the least χ2, RMSE and mean relative deviation modulus (E. Wang and Singh model, having χ2 and RMSE value (at 60 °C of 0.00027 and 0.01655 respectively gave the best results for describing the drying behavior of jackfruit samples.

  12. Artocarpus heterophyllus Lam. (lackfruit tree; Hindi: Katha!) of ...

    Indian Academy of Sciences (India)

    The entire female inflorescence together with parts of individual flowers forms a large multiple fruit. The sepals and petals become fleshy and juicy to form the edible part of the fruit. The timber is hard and durable and usedfor making various wooden articles. The leaves are used in skin diseases and as an antidote to snake ...

  13. Productivity of a plantation of asparagus (Asparagus officinalis var. Altilis initiated green seedlings over four growing seasons

    Directory of Open Access Journals (Sweden)

    Castagnino, A.M.

    2014-01-01

    Full Text Available Although there are two initiation systems for an asparagus crop, i.e. by means of crowns and by means of seedlings, only the former allows the initiation of the definitive plantation in the first year. In order to determine the incidence of the seedling size throughout the first six years since planting, a trial was started on august 21, 2002, with three cell sizes: PG: big, PM: medium and PCH: small (70, 50 and 20 cm3 respectively and two densities: D1:35,714 and D2: 17,857 pl.ha-1, using the hybrid UC-157 and evaluating its productivity in the period 2005- 2008. Fresh weight per harvest (PFC and total annual fresh weight per ha (PFT, number of turions per harvest (NTC and per ha (NT and average weight per turion (PPT were determined. The PTF 2005-2008 was 26.300 Kg ha-1 and NT, with an annual average of 6,575 Kg ha-1 and 480,180 turions, respectively. NT showed a growing tendency throughout the whole period. Whereas, while the PFT grew in 2005-2007, it decreased in 2008 due to lower PPT, caused possibly by intra-specific competition. PM allowed a greater yield PFC: 343 Kg ha-1. The PPT turned out to be similar to that in 2005-2007, however it decreased in 2008. D1 obtained the maximum yield throughout the whole period. It can be concluded that when the aim is to obtain high initial productivity, it would be advisable to use seedlings of good size. Besides, the choice of density is an important factor due to the fact that there seems to be a direct relationship between the planting frame used and the obtained productivity.

  14. Toxicological parameters of albino rats fed with extruded snacks from Aerial yam (Dioscoria bulbifera) and African breadfruit seed (Treculia africana).

    Science.gov (United States)

    Olatoye, Kazeem K; Arueya, Gibson L

    2018-01-01

    In this study, safety of novel food from aerial yam and Treculia africana , underutilized food materials with high-nutritive value and health benefits were investigated. Animal experiment involving the use of thirty (30) male albino rats was conducted for 28 days.Thereafter, rats in all groups were sacrificed and blood samples collected for biochemical analysis and hematological assay. Some vital organs were harvested and used for histological analysis. Biochemical and hematological parameters were not significantly p  ≤ .05 different among the treatment and controls. However there was an increase in monocytes, which is a reflection of immune boosting potential of the novel snack. No significant pathological changes were observed in liver and kidney of rats fed with this snack. Rats showed no signs of toxicity within the study period. These findings suggest that product may be safe and useful as an Immune adjuvant.

  15. PLANTAS ÚTILES EN LOS SISTEMAS AGROFORESTALES TRADICIONALES DEL LITORAL ECUATORIANO

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    Luz García Cruzatty

    2008-12-01

    Full Text Available El estudio se realizó en la Cuenca Media del Río Guayas, Ecuador, en el área correspondiente a los cantones Mocache y Palenque. El inventario de las especies se obtuvo mediante recorridos participativos. Las especies se clasificaron de acuerdo al Sistema Filogenético de Cronquist. Los datos sobre el uso de las especies se obtuvieron mediante charlas y entrevistas no formales. Se determinó que los agricultores de la Cuenca Media del Río Guayas conocen y utilizan 262 especies vegetales que pertenecen a 67 familias y 164 géneros. Las familias vegetales más comunes fueron: Anacardiaceae, Papilonaceae, Solanaceae, Rutaceae, Musaceae y Lamiaceae, con más de diez especies cada una. Se documentaron 21 tipos de usos, de los cuales los más importantes son: alimenticio, medicinal y construcción, lo que demuestra la preocupación de las familias por satisfacer sus necesidades básicas. La mayoría de las especies utilizadas son árboles, seguido por hierbas y arbustos. Entre las especies de mayor importancia por los múltiples usos y productos que se obtienen están: Persea americana Mill. (aguacate, Artocarpus altilis Fosberg (fruta de pan, Musa sp. (plátano, Manguifera sp. (mango, Phythelephas aequatoriales Spruce (cade, Inga edulis Matius (guaba de bejuco, entre otros.

  16. OPTIMASI PROSES EKSTRAKSI PEKTIN DAMI BUAH NANGKA (Artocarpus heterophyllus Lamk Optimizing of Pectin Extraction Process from Jackfruit Rags (Artocarpus heterophyllus Lamk

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    I Nengah Kencana Putra

    2012-05-01

    Full Text Available The objective of this research was to determine the optimum  condition  on extraction process of jackfruit rags pectin. The experiment was designed by Randomized Block Design (RBD within 3 x 3 factorial experiment. The first factorwas pH of solvent consisted of 3 levels: 1.5, 2.5, and 3,5. The second factor was ratio of jackfruit rags to solvent, con-sisted of 3 levels: 1:5, 1:6 and 1:7. Results repealed that the solvent pH effected significantly yield, total ash, equivalentweigh and anhydrogalacturonic acid content of pectin produced. The optimum conditions for jackfruit rags pectin extraction were: the pH of extracting solvent was 1.5, and the ratio of jackfruit rags to solvent was 1:5. Those condi- tions gave the yield of 4.45 %, produced pectin having total ash (2.82 %, equivalent  weigh (3,022.24 g/eqi, methoxylcontent (8.16 %, and anhydrogalacturonic acid content (88.01 %. ABSTRAK Penelitian ini bertujuan untuk menentukan kondisi optimal pada proses ekstraksi pektin dami buah nangka. Rancanganpenelitian  yang digunakan adalah Rancangan Acak �elompok (RA� dengan pola percobaan faktorial  3 x 3. Faktorpertama adalah pH cairan pengekstrak yang terdiri dari 3 taraf: pH 1,5; 2,5 dan 3,5. Faktor kedua adalah perbandingandami buah nangka dengan cairan pengekstrak yang terdiri dari 3 taraf: 1:5, 1:6 dan 1:7. Hasil penelitian menunjukkan pH cairan pengekstrak berpengaruh nyata terhadap rendemen, kadar abu, berat ekivalen dan kadar asam anhidrogalak- turonat. Kondisi optimal untuk ekstraksi pektin dami buah nangka adalah: cairan pengekstrak pH 1,5, dengan perband- ingan dami nangka dan cairan pengekstrak 1:5. Kondisi ini menghasilkan rendemen 4,54 %, serta pektin dengan kara- kteristik: kadar abu 2,82 %, berat ekivalen 3.022,24 g/eki, kadar metoksil 8,16 % dan kadar asam anhidrogalakturonat88,01 %.

  17. Production of spray-dried honey jackfruit (Artocarpus heterophyllus) powder from enzymatic liquefied puree.

    Science.gov (United States)

    Wong, Chen Wai; Tan, Hong Hock

    2017-02-01

    This paper presents the enzymatic liquefaction process for honey jackfruit optimized with Pectinex ® Ultra SP-L and Celluclast ® 1.5 L individually or in combinations at different concentrations (0-2.5% v/w) and incubation time (0-2.5 h). Treatment with combinations of enzymes showed a greater effect in the reduction of viscosity (83.9-98.8%) as compared to single enzyme treatment (64.8-87.3%). The best parameter for enzymatic liquefaction was obtained with 1.0% (v/w) Pectinex ® Ultra SP-L and 0.5% (v/w) Celluclast ® 1.5 L for 1.5 h. Spray drying process was carried out using different inlet temperatures (140-180 °C) and maltodextrin concentrations (10-30% w/w). Results indicated that the spray-dried honey jackfruit powder produced at 160 °C with 30% w/w maltodextrin gave the highest product yield (66.90%) with good powder qualities in terms of water activity, solubility, moisture content, hygroscopicity, color and bulk density. The spray-dried honey jackfruit powder could potentially be incorporated into various food products.

  18. Chemical, morphological and functional properties of Brazilian jackfruit (Artocarpus heterophyllus L.) seeds starch.

    Science.gov (United States)

    Madruga, Marta Suely; de Albuquerque, Fabíola Samara Medeiros; Silva, Izis Rafaela Alves; do Amaral, Deborah Silva; Magnani, Marciane; Queiroga Neto, Vicente

    2014-01-15

    Starches used in food industry are extracted from roots, tubers and cereals. Seeds of jackfruit are abundant and contain high amounts of starch. They are discarded during the fruit processing or consumption and can be an alternative source of starch. The starch was extract from the jackfruit seeds and characterised to chemical, morphological and functional properties. Soft and hard jackfruit seeds showed starch content of 92.8% and 94.5%, respectively. Starch granules showed round and bell shape and some irregular cuts on their surface with type-A crystallinity pattern, similar to cereals starches. The swelling power and solubility of jackfruit starch increased with increasing temperature, showing opaque pastes. The soft seeds starch showed initial and final gelatinisation temperature of 36°C and 56°C, respectively; while hard seeds starch presented initial gelatinisation at 40°C and final at 61°C. These results suggest that the Brazilian jackfruit seeds starches could be used in food products. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Carotenoid composition of jackfruit (Artocarpus heterophyllus), determined by HPLC-PDA-MS/MS.

    Science.gov (United States)

    de Faria, A F; de Rosso, V V; Mercadante, A Z

    2009-06-01

    Carotenoids are pigments responsible for the yellow-reddish color of many foods and are related to important functions and physiological actions, preventing several chronic-degenerative diseases. The objective of this study was to confirm the carotenoid composition of jackfruit by high-performance liquid chromatography connected to photodiode array and mass spectrometry detectors (HPLC-PDA-MS/MS). The main carotenoids were all-trans-lutein (24-44%), all-trans-beta-carotene (24-30%), all-trans-neoxanthin (4-19%), 9-cis-neoxanthin (4-9%) and 9-cis-violaxanthin (4-10%). Either qualitative or quantitative differences, mainly related to the lutein proportion, were found among three batches of jackfruit. Since the fruits from batch A showed significantly lower contents for almost all carotenoids, it also had the lowest total carotenoid content (34.1 microg/100 g) and provitamin A value, whereas the total carotenoid ranged from 129.0 to 150.3 microg/100 g in the other batches. The provitamin A values from batches B and C were 3.3 and 4.3 microg RAE/100 g, respectively. The carotenoid composition of jackfruit was successfully determined, where 14 of the 18 identified carotenoids were reported for first time. Differences among batches may be due to genetic and/or agricultural factors.

  20. PENGARUH PENGGUNAAN TEPUNG BIJI NANGKA (Artocarpus heterophyllus DALAM PAKAN TERHADAP BOBOT GIBLET AYAM PEDAGING

    Directory of Open Access Journals (Sweden)

    Ulfa Tiara Pangesti

    2017-04-01

    Full Text Available The purpose of this study was to determine the effect used of jackfruit seed meal in diet on broiler giblet weights. The materials of this study were 100 birds of New Lohman MB-202 P broiler chicks with average 37,77 unsexing. The method of this experiment was Completly Randomize Design (CRD with 4 treatment and 5 replication, if there were significant effect, it would tested by Duncan’s Multiple Range Test Method, and continued with Simple Linear Regression. The result showed that the jackfruit seed meal in broiler diet gave significant effect (P>0,01 on final weight, heart weight, liver weight, gizzard weight and spleen weight. The jackfruit seed meal had a negatif correlation with final weight, heart weight, liver weight, gizzard weight and spleen weight. It had conclude that jackfruit seed meal can be used up to the level of  5% in broiler diet, viewed from the final weight, weight of the heart, liver, gizzard, and spleen, because the weights are not significantly different with diet without the use of jackfruit seed meal. Keywords : Broiler, Jackfruit Seed Meal, Giblet

  1. Effect of Vacuum Frying on Changes in Quality Attributes of Jackfruit (Artocarpus heterophyllus Bulb Slices

    Directory of Open Access Journals (Sweden)

    Tanushree Maity

    2014-01-01

    Full Text Available The effect of frying temperatures and durations on the quality of vacuum fried jackfruit (JF chips was evaluated. Moisture content and breaking force of JF chips decreased with increase in frying temperature and time during vacuum frying whereas the oil content increased. The frying time for JF chips was found to be 30, 25, and 20 minutes at 80, 90, and 100°C, respectively. JF chips fried at higher temperature resulted in maximum shrinkage (48%. The lightness in terms of hunter L* value decreased significantly (P<0.05 during frying. Sensory evaluation showed maximum acceptability for JF chips fried at 90°C for 25 min. Frying under vacuum at lower temperatures was found to retain bioactive compounds such as total phenolics, total flavonoids, and total carotenoids in JF chips. Almost 90% of carotenoids were lost from the samples after 30 min of frying at 100°C.

  2. The isolation and characterisation of jacalin [Artocarpus heterophyllus (jackfruit) lectin] based on its charge properties.

    Science.gov (United States)

    Kabir, S

    1995-02-01

    Jackfruit extracts contain a protein termed jacalin which possesses diverse biological properties. A detailed analysis of its charge properties has been lacking. The present investigation was initiated to study isoelectric properties of jacalin in detail and to isolate a single isoform of jacalin. Jacalin was isolated from jackfruit extracts by affinity chromatography on immunoglobulin-A immobilised to Sepharose 4B. Various techniques such as ion-exchange chromatography, isoelectric focusing (IEF) on polyacrylamide gels and preparative liquid IEF with the Rotofor cell were used. When analysed by IEF on thin layer polyacrylamide gels, jacalin was resolved into 35 bands over a pH range of 5.0-8.5. Upon SDS-PAGE in the second dimension all these charge species gave rise to only two-bands at 12 and 15.4 kDa. The lectin was mostly eluted with 50 and 100 mM sodium chloride when jackfruit extracts were fractionated on an anion-exchange column of DEAE-cellulose. In a single 6 hour run by preparative IEF with the Rotofor cell in the pH range of 3-9.5, it has been possible to isolate pure jacalin fractions containing fewer number of charged isomers. A single jacalin isoform was isolated by subjecting a Rotofor fraction containing fewer charged species to preparative IEF on thin layer polyacrylamide gel and eluting the band of interest from the gel. The isolated jacalin isoform was biologically active as it agglutinated erythrocytes. The study reveals the complexity of jacalin as it exists as multiple charge isomers over a broad pH range. By performing preparative IEF in solution as well as in thin layer polyacrylamide gels, it was possible to isolate a single jacalin isoform with the retention of biological activity.

  3. PENGARUH PEMBERIAN JUMLAH TAKARAN RAGI TERHADAP KANDUNGAN PROTEIN YANG DIHASILKAN PADA TEMPE BIJI NANGKA (Artocarpus heterophyllus

    Directory of Open Access Journals (Sweden)

    Harmoko Harmoko

    2016-05-01

    Full Text Available Jackfruit (Artocarpusheterophyllus, is well known in Indonesia, when the fruit is ripe jackfruit can be consumed. After we consume jackfruit, we'll leave the trash in the form of jackfruit seed, which has not been used by the community to the fullest. The purpose of this study are trying to explore another potential of jackfruit seeds and increasing the protein content of jackfruit seeds by processing  in to tempeh. Another purpose is knowing the number yeast of doses that can produce in tempeh of jackfruit seeds. This research uses 4 treatments (A: 0.25 grams yeast/50 grams jackfruit seed, B: 0.50 grams yeast/50 grams jackfruit seeds, C: 0.75 gram yeast/50 grams jackfruit seeds, and D: 1 grams yeast/50 grams of jackfruit seeds with 4 repetitions. Test protein content tests conducted at the Laboratory of Food Technology and Agricultural Faculty of Agricultural Technology, GadjahMada University (UGM. Treatment A produced protein average of 2,64%, B produced protein average of 3,429%, treatment C produced protein average of 4,146% and treatment D produced protein average of 5,635% protein. The highest protein content is produced by treatment D. Keywords: Takaran   ragi, tempe  biji  nangka (Artocarpusheterophyllus,kandungan protein.

  4. Purification and characterization of polyphenol oxidase from jackfruit ( Artocarpus heterophyllus ) bulbs.

    Science.gov (United States)

    Tao, Yi-Ming; Yao, Le-Yi; Qin, Qiu-Yan; Shen, Wang

    2013-12-26

    Polyphenol oxidase (PPO) from jackfruit bulb was purified through acetone precipitation, ion-exchange column, and gel filtration column. PPO was a dimer with the molecular weight of 130 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration. The Km was 8.3 and 18.2 mM using catechol and 4-methylcatechol as substrates, respectively. The optimum pH was 7.0 (catechol as the substrate) or 6.5 (4-methylcatechol as the substrate). The optimum temperature was 8 °C. The enzyme was stable below 40 °C. The activation energy (Ea) of heat inactivation was estimated to be 103.30 kJ/mol. The PPO activity was activated by Mn(2+), SDS, Tween-20, Triton X-100, citric acid, and malic acid but inhibited by K(+), Zn(2+), Mg(2+), Ca(2+), Ba(2+), cetyl trimethyl ammonium bromide (CTAB), kojic acid, tropolone, glutathione (GSH), cysteine (Cys), and ascorbic acid (AA). Cys and AA were effective to reduce browning of jackfruit bulbs during the storage at 8 °C for 15 days.

  5. Effect of Vacuum Frying on Changes in Quality Attributes of Jackfruit (Artocarpus heterophyllus) Bulb Slices.

    Science.gov (United States)

    Maity, Tanushree; Bawa, A S; Raju, P S

    2014-01-01

    The effect of frying temperatures and durations on the quality of vacuum fried jackfruit (JF) chips was evaluated. Moisture content and breaking force of JF chips decreased with increase in frying temperature and time during vacuum frying whereas the oil content increased. The frying time for JF chips was found to be 30, 25, and 20 minutes at 80, 90, and 100°C, respectively. JF chips fried at higher temperature resulted in maximum shrinkage (48%). The lightness in terms of hunter L (*) value decreased significantly (P < 0.05) during frying. Sensory evaluation showed maximum acceptability for JF chips fried at 90°C for 25 min. Frying under vacuum at lower temperatures was found to retain bioactive compounds such as total phenolics, total flavonoids, and total carotenoids in JF chips. Almost 90% of carotenoids were lost from the samples after 30 min of frying at 100°C.

  6. KARAKTERISTIK SENSORIS DAN KIMIA PADA ABON NANGKA MUDA (Artocarpus heterophyllus LMK DENGAN PENAMBAHAN TEMPE

    Directory of Open Access Journals (Sweden)

    Ulfa Qurrota A’yuni Nur Jannah

    2016-12-01

    Full Text Available Unripe jackfruit is one vegetables that  is usually processed into Indonesian dishs. Beside unripe jackfruit processed into vegetables can also be developed and processed into a dry product called beef floss. Unripe jackfruit has a very low protein content, so it needs other raw material to improve protein content of the floss we added tempeh to the floss = gembus tempeh or soybean tempeh, as much as 15%, 25%, 35% and 0% as control. This study uses a Completely Randomized Design (CRD with 2 factors. The first factor is proportion of unripe jackfruit and tempeh, and the second factor is type of tempeh. Parameter of this research are organoleptic evaluation, the color, moisture content and protein content. The result of organoleptic evaluation is the proportion 25% soybean tempeh prefered for aroma, taste, color, texture. The highest L (Lightness was resulted from 35% soybean tempeh. The highest moisture content was shown by floss with 15% soybean tempeh. Protein content increased from 4,3775% to 39,3975% by addition of soybean tempeh while protein increased from 4,3775%  to 14,8835% in beef floss added with gembus  tempeh.

  7. Pengaruh Penambahan Jerami Nangka (Artocarpus heterophyllus Lam Terhadap Karakteristik Fruit Leather Mangga (Mangifera indica L

    Directory of Open Access Journals (Sweden)

    Lisa Yusmita

    2018-04-01

    Full Text Available ABSTRAK Penganekaragaman pangan merupakan program kebijakan pemerintah dengan tujuan untuk terus mengembangkan pangan agar dapat memantapkan kemandirian dan penyediaan pangan dengan jenis yang beragam dan kualitas yang makin meningkat, jumlah yang memadai dan tersebar merata sehingga dapat memenuhi kebutuhan pangan dan gizi. Untuk mendukung penganekaragaman pangan tersebut maka diperlukan upaya untuk melakukan pengolahan produk-produk hasil pertanian. Buah-buahan merupakan salah satu produk hasil pertanian yang umumnya dibuat menjadi produk olahan seperti jam, jelly, puree, sari buah, buah kaleng, manisan kering atau basah. Salah satu jenis produk buah-buahan yang kering selain manisan adalah fruit leather. Fruit leather adalah jenis makanan yang berasal dari daging buah yang telah dihancurkan dan dikeringkan. Produk ini berbentuk lembaran tipis seperti halnya kulit buah dengan tekstur yang plastis dan kenyal, rasanya manis tetapi masih memiliki ciri rasa khas buah yang digunakan. Fruit leather juga termasuk produk makanan yang tidak mengandung zat pewarna sehingga cocok untuk dijadikan cemilan dan mempunyai aneka ragam bentuk dan warna. Pengolahan buah-buahan menjadi fruit leather merupakan salah satu upaya divesifikasi pangan. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL dengan 5 perlakuan dan 3 kali ulangan. Data yang diperoleh dianalisa secara statistik dengan uji F, kemudian bila berpengaruh nyata dilanjutkan dengan uji DNMRT pada taraf nyata 5%. Penelitian ini telah dilakukan di Laboratorium Program Studi Teknologi Industri Pertanian Universitas Dharma Andalas dan Laboratorium Teknologi Hasil Pertanian Fakultas Teknologi Pertanian Universitas Andalas. Dari hasil penelitian didapatkan kesimpulan bahwa konsentrasi penambahan jerami nangka berpengaruh nyata terhadap kadar vitamin C dan kadar serat kasar. Sementara untuk pengujian kadar air, total asam dan kadar gula ternyata tidak dipengaruhi oleh konsentrasi penambahan jerami nangka.   Kata kunci : fruit Leather, jerami nangka, mangga.

  8. Functional properties and utilization of Artocarpus heterophyllus Lam seed starch from new species in China.

    Science.gov (United States)

    Zhang, Yanjun; Hu, Meijie; Zhu, Kexue; Wu, Gang; Tan, Lehe

    2018-02-01

    Jackfruit is now receiving extensive attention as a new source of starch. However, jackfruit seeds are discarded as waste, although they are rich in starch. The functional properties of the starches were investigated from new Chinese jackfruit species. All the starches have a high amylose (26.56-38.34%) with a potential to become functional foods rich in resistant starch. The jackfruit starches varied from trigonal and tetragonal, round to semi-oval/bell shapes and showed significant variations in particle sizes (5.53-14.46μm). These variations led to significant differences in their functional properties, and significant correlations were found in their pasting, thermal, crystal and texture parameters. Hierarchical cluster analysis sorted the samples into three groups of 1) Malaysia 8 (M8) and ZhenZhu (ZZ); 2) Malaysia 2, Malaysia 3 and Malaysia 4, (M2, M3, M4); and 3) Xiangyinsuo 11, Xiangyinsuo 4, Xiangyinsuo 3 and Xiangyinsuo 2 (X11, X4, X3, X2). The first group could be used as food thickening or gelling agents. The second group could be applied in glutinous foods. The third group make them suitable for fillings in confectionery or weaning foods. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Characteristics of Red Algae Bioplastics/Latex Blends under Tension

    Directory of Open Access Journals (Sweden)

    M. Nizar Machmud

    2013-10-01

    Full Text Available Cassava, corn, sago and the other food crops have been commonly used as raw materials to produce green plastics. However, plastics produced from such crops cannot be tailored to fit a particular requirement due to their poor water resistance and mechanical properties. Nowadays, researchers are hence looking to get alternative raw materials from the other sustainable resources to produce plastics. Their recent published studies have reported that marine red algae, that has been already widely used as a raw material for producing biofuels, is one of the potential algae crops that can be turned into plastics. In this work, Eucheuma Cottonii, that is one of the red alga crops, was used as raw material to produce plastics by using a filtration technique. Selected latex of Artocarpus altilis and Calostropis gigantea was separately then blended with bioplastics derived from the red algae, to replace use of glycerol as plasticizer. Role of the glycerol and the selected latex on physical and mechanical properties of the red algae bioplastics obtained under a tensile test performed at room temperature are discussed. Tensile strength of some starch-based plastics collected from some recent references is also presented in this paperDoi: 10.12777/ijse.5.2.81-88 [How to cite this article: Machmud, M.N., Fahmi, R.,  Abdullah, R., and Kokarkin, C.  (2013. Characteristics of Red Algae Bioplastics/Latex Blends under Tension. International Journal of Science and Engineering, 5(2,81-88. Doi: 10.12777/ijse.5.2.81-88

  10. Leaf litter breakdown rates and associated fauna of native and exotic trees used in Neotropical Riparia Reforestation; Tasas de perdida de masa de la hojarasca y fauna asociada en especies de arboles comunmente utilizados en la Reforestacion de Riberas Neotropicales

    Energy Technology Data Exchange (ETDEWEB)

    Gutierrez Isaza, Nataly; Blanco, Juan Felipe

    2014-07-01

    A signature of globalization is the prevalence of exotic trees along reforested urban and rural riparian zones in the neotropics, but little is known about the instream processing of its leaf litter. In this study, leaf litter breakdown rates were measured during 35 days using mesh bags within a reference headwater stream for seven exotic and three native tree species commonly used in urban and rural reforestation. Artocarpus altilis, Schefflera actinophylla and Terminalia catappa scored the highest mass loss rates (>85 %; mean life: t50 <15 d), while Cecropia sp. and Cespedesia macrophylla (mass loss =36 and 15 %; t50 =58 and 172 d, respectively) scored the lowest rates. However, a broad range of rates was observed among the ten species studied. The carbon to phosphorus ratio (c:p) and toughness of the leaf litter were the best predictors of breakdown rates. However, these leaf properties were not correlated with the very low values of macro invertebrates abundance and diversity, and the few morpho classified as shredders. Therefore physical rather than biological controls seem to best explain the observed variability of mass loss rates, and thus slow decomposing leaf litter species seems to provide a habitat rather than a food resource, particularly to collectors. This study suggests that riparian reforestation will propagate species-specific ecological influences on instream processes such as leaf litter processing depending on leaf quality properties, therefore ecosystem-wide influences should be considered for improving reforestation strategies. Future studies should test for differences in breakdown rates and colonization by macro invertebrates relative for leaf litter species origin (native vs. exotic).

  11. Leaf litter breakdown rates and associated fauna of native and exotic trees used in Neotropical Riparia Reforestation

    International Nuclear Information System (INIS)

    Gutierrez Isaza, Nataly; Blanco, Juan Felipe

    2014-01-01

    A signature of globalization is the prevalence of exotic trees along reforested urban and rural riparian zones in the neotropics, but little is known about the instream processing of its leaf litter. In this study, leaf litter breakdown rates were measured during 35 days using mesh bags within a reference headwater stream for seven exotic and three native tree species commonly used in urban and rural reforestation. Artocarpus altilis, Schefflera actinophylla and Terminalia catappa scored the highest mass loss rates (>85 %; mean life: t50 <15 d), while Cecropia sp. and Cespedesia macrophylla (mass loss =36 and 15 %; t50 =58 and 172 d, respectively) scored the lowest rates. However, a broad range of rates was observed among the ten species studied. The carbon to phosphorus ratio (c:p) and toughness of the leaf litter were the best predictors of breakdown rates. However, these leaf properties were not correlated with the very low values of macro invertebrates abundance and diversity, and the few morpho classified as shredders. Therefore physical rather than biological controls seem to best explain the observed variability of mass loss rates, and thus slow decomposing leaf litter species seems to provide a habitat rather than a food resource, particularly to collectors. This study suggests that riparian reforestation will propagate species-specific ecological influences on instream processes such as leaf litter processing depending on leaf quality properties, therefore ecosystem-wide influences should be considered for improving reforestation strategies. Future studies should test for differences in breakdown rates and colonization by macro invertebrates relative for leaf litter species origin (native vs. exotic).

  12. Impact of gamma irradiation on biochemical attributes of jackfruit (Artocarpus heterophyllus L.) seeds during different storage period

    International Nuclear Information System (INIS)

    Adiga, Madhavi; Akshatha, Madhuri Adiga; Chandrashekar, K.R.

    2014-01-01

    Seeds of A. heterophyllus Lam. were exposed to different (0.5-2.5 kGy) doses of gamma radiation and stored for 3 weeks under ambient condition for observation of biochemical changes. The significant increase in phenolic content was observed at 2.5 kGy compared to control. Increase in protein content was observed at 1.5 and 2kGy at the end of 3 rd week. Increased total soluble sugar content was observed with increased dose. Enhancement in antioxidant activity at 2kGy may be due to the increased phenolic content. (author)

  13. Pengaruh Penambahan 1,4-Butanadiol dan Polietilen Glikol (PEG 1000 terhadap Kemudahan Biodegradasi Bioplastik dari Biji Nangka (Artocarpus heterophyllus

    Directory of Open Access Journals (Sweden)

    Argo Khoirul Anas

    2016-10-01

    Full Text Available The influences of plasticizer on the biodegradability of bioplastic film synthesized from starch obtained from jackfruit seeds with a help of Acetobacter xylinum were investigated. In this study, 1,4-butanediol and polyethylene glycol (PEG 1000 were used as plasticizer. The biodegradation behavior of the resulted bioplastic films was characterized by calculating changes in their mass loss and rate of mass loss. The Fourier Transform Infrared (FTIR spectroscopy was also conducted to analyze theirfunctional group. The results shows that all the bioplastic films were well biodegradated, where PEG 1000 with concentration of 0,5% and 1,0% can successfully improve the biodegradability of bioplasticfilm. Meanwhile, the used of 1,4-butanediol with concentration of 0,5% and 1,0% can increase and decrease the biodegradability of sample, respectively.

  14. Effect of high-intensity ultrasound on the technofunctional properties and structure of jackfruit (Artocarpus heterophyllus) seed protein isolate.

    Science.gov (United States)

    Resendiz-Vazquez, J A; Ulloa, J A; Urías-Silvas, J E; Bautista-Rosales, P U; Ramírez-Ramírez, J C; Rosas-Ulloa, P; González-Torres, L

    2017-07-01

    The influence of high-intensity ultrasound (HIU) on the technofunctional properties and structure of jackfruit seed protein isolate (JSPI) was investigated. Protein solutions (10%, w/v) were sonicated for 15min at 20kHz to the following levels of power output: 200, 400, and 600W (pulse duration: on-time, 5s; off-time 1s). Compared with untreated JSPI, HIU at 200W and 400W improved the oil holding capacity (OHC) and emulsifying capacity (EC), but the emulsifying activity (EA) and emulsion stability (ES) increased at 400W and 600W. The foaming capacity (FC) increased after all HIU treatments, as opposed to the water holding capacity (WHC), least gelation concentration (LGC), and foaming stability (FS), which all decreased except at pH 4 for FS. Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) showed changes in the molecular weight of protein fractions after HIU treatment. Scanning electron microscopy (SEM) demonstrated that HIU disrupted the microstructure of JSPI, exhibiting larger aggregates. Surface hydrophobicity and protein solubility of the JSPI dispersions were enhanced after ultrasonication, which increased the destruction of internal hydrophobic interactions of protein molecules and accelerated the molecular motion of proteins to cause protein aggregation. These changes in the technofunctional and structural properties of JSPI could meet the complex needs of manufactured food products. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Pengaruh Penambahan 1,4-Butanadiol dan Polietilen Glikol (PEG) 1000 terhadap Kemudahan Biodegradasi Bioplastik dari Biji Nangka (Artocarpus Heterophyllus)

    OpenAIRE

    Anas, Argo Khoirul; Ariefta, Nanang Rudianto; Nurfiana, Yuni; Rohaeti, Eli

    2016-01-01

    The influences of plasticizer on the biodegradability of bioplastic film synthesized from starch obtained from jackfruit seeds with a help of Acetobacter xylinum were investigated. In this study, 1,4-butanediol and polyethylene glycol (PEG) 1000 were used as plasticizer. The biodegradation behavior of the resulted bioplastic films was characterized by calculating changes in their mass loss and rate of mass loss. The Fourier Transform Infrared (FTIR) spectroscopy was also conducted to analyze ...

  16. EFECTO DEL 1-METILCICLOPROPENO (1-MCP SOBRE LA FISIOLOGÍA Y CALIDAD DE FRUTOS DE JACA (Artocarpus heterophyllus Lam.

    Directory of Open Access Journals (Sweden)

    M. Mata-Montes de Oca

    2007-01-01

    Full Text Available Debido a sus características de color y sabor de pulpa, la demanda de fruta de jaca se ha incrementado considerablemente. La mayoría de la producción de Nayarit es exportada a los Estados Unidos de Norteamérica. Sin embargo, debido a su corta vida de anaquel y susceptibilidad al daño por frío la fruta es exportada vía aérea con altos costos de flete. Se reporta que el 1-Metilciclopropeno (1-MCP retarda el proceso de madurez, alarga la vida de anaquel y mantiene la calidad de varios frutales, así que el objetivo de este estudio fue evaluar el efecto del 1-MCP sobre la fisiología y calidad del fruto de jaca almacenado a 20 ºC. Las frutas fueron cosechadas en madurez fisiológica, lavadas y tratadas con 1-MCP a 100 y 300 nl·litro-1 en cámaras selladas por 12 h a 20 ± 1 ºC; 85 ± 5 % de HR manteniendo un testigo sin tratar. Las variables evaluadas fueron: velocidad de respiración y de producción de etileno, sólidos solubles totales, acidez titulable, firmeza de la pulpa y de la fruta entera, color en la pulpa y pérdida de peso. El 1-MCP no afectó la mayoría de las variables de calidad pero retrasó significativamente la velocidad de respiración y la producción de etileno así como el ablandamiento de la pulpa, lo que permitió prolongar la vida de anaquel hasta por ocho y 12 días a 100 y 300 nl·litro-1, respectivamente.

  17. Perbedaan respon Aedes aegypti (Linnaeus (Diptera: Culicidae, terhadap paparan anti nyamuk bakar dan bunga keluwih (Artocarpus camansi, Blanco

    Directory of Open Access Journals (Sweden)

    Nur Endah Wahyuningsih

    2015-09-01

    Full Text Available The control of dengue hemorrhagic fever (DHF it is important to control the vector, i.e. Aedes aegypti (Linnaeus and Ae. albopictus (Skuse. Usually, controls of these vectors are done using chemical insecticides. Research on mosquito resistance has been done, but the impact of mosquitoes that survive after synthetic (chemical insecticides application has not been studied. The aim of this research was to analyze the differences of fecundity, fertility and vitality rate of mosquitoes that were exposed to natural insecticides i.e with burned breadnut flowers and mosquito coils containing transflutrin and d-aletrin. Mosquito samples were taken from field eggs which were exposed with synthetic and natural insecticides. Mosquitoes that remainder alive after 24 hours exposure were mated and each group were put in 2 cages, each repetition were repeated 3 times. The mosquito eggs were observed until adult mosquitoes die. Then, the fecundity, fertility and vitality rate were compared. Based on our analysis, there were no differences on fecundity rate, between control and mosquitoes that were exposed to natural insecticides, but there were significant differences between the control and the synthetic insecticides. Natural insecticides do not increase the rate of fecundity but synthetic insecticides may increase the rate of fecundity and number of mosquitoes and causes the mosquito lifespan longer than the control and natural insecticide treatment.

  18. Pemanfaatan Tepung Biji Cempedak ( Artocarpus Chempeden dan Tepung Biji Durian ( Durio Zhibetinus Murr ) Dalam Pembuatan Bakso Ikan

    OpenAIRE

    Susianti, Epi

    2014-01-01

    ‘Cempedak’ and durian seeds flour are the kind of seeds that are less utilized as a product of the food industry. ‘Cempedak’ and durian seeds flour can be processed into various food product which can give contribute nutrient for schoolchildren. One of the product is fish meatball. This study aims to determine organoleptic and nutritional of modified fish meatball with ‘cempedak’ and durian seed’s flour. This experimental design was completely randomized design with of two factors are ...

  19. Perbedaan respon Aedes aegypti (Linnaeus) (Diptera: Culicidae), terhadap paparan anti nyamuk bakar dan bunga keluwih (Artocarpus camansi, Blanco)

    OpenAIRE

    Nur Endah Wahyuningsih; Ramauli Agustina Sihit

    2015-01-01

    The control of dengue hemorrhagic fever (DHF) it is important to control the vector, i.e. Aedes aegypti (Linnaeus) and Ae. albopictus (Skuse). Usually, controls of these vectors are done using chemical insecticides. Research on mosquito resistance has been done, but the impact of mosquitoes that survive after synthetic (chemical) insecticides application has not been studied. The aim of this research was to analyze the differences of fecundity, fertility and vitality rate of mosquitoes that w...

  20. Extracts of Artocarpus communis Induce Mitochondria-Associated Apoptosis via Pro-oxidative Activity in Human Glioblastoma Cells

    Directory of Open Access Journals (Sweden)

    Chiang-Wen Lee

    2018-05-01

    Full Text Available Glioblastoma multiforme (GBM is an extremely aggressive and devastating malignant tumor in the central nervous system. Its incidence is increasing and the prognosis is poor. Artocarpin is a natural prenylated flavonoid with various anti-inflammatory and anti-tumor properties. Studies have shown that artocarpin is associated with cell death of primary glioblastoma cells. However, the in vivo effects and the cellular and molecular mechanisms modulating the anticancer activities of artocarpin remain unknown. In this study, we demonstrated that treating the glioblastoma cell lines U87 and U118 cells with artocarpin induced apoptosis. Artocarpin-induced apoptosis is associated with caspase activation and poly (ADP-ribose polymerase (PARP cleavage and is mediated by the mitochondrial pathway. This is associated with mitochondrial depolarization, mitochondrial-derived reactive oxidative species (ROS production, cytochrome c release, Bad and Bax upregulations, and Bcl-2 downregulation. Artocarpin induced NADPH oxidase/ROS generation plays an important role in the mitochondrial pathway activation. Furthermore, we found artocarpin-induced ROS production in mitochondria is associated with Akt- and ERK1/2 activation. After treatment with artocarpin, ROS causes PI3K/Akt/ERK1/2-induced cell death of these tumor cells. These observations were further verified by the results from the implantation of both U87 and U118 cells into in vivo mouse. In conclusion, our findings suggest that artocarpin induces mitochondria-associated apoptosis of glioma cells, suggesting that artocarpine can be a potential chemotherapeutic agent for future GBM treatment.

  1. Pembuatan “Fruit Leather” Buah Jeruk Manis (Citrussinensis L.)dengan Penambahan Dami Nangka (Artocarpus Heterophyllus)

    OpenAIRE

    Hasibuan, Sarah Siska; Harun, Noviar; Ali, Akhyar

    2017-01-01

    The Purpose of this research is to find the best treatment from the production of sweet orange and jackfruit combination Fruit Leather. This research using Completely Randomized Design with 4 treatment and 4 repetition. This treatment are combining 85 grams sweet orange mush and 15 grams jackfruit (JD1); 65 grams sweet orange mush and 35 grams jackfruit (JD2); 45 grams sweet oranges mush and 55 grams jackfruit (JD3); 25 grams sweet oranges mush and 75 grams jackfruit (JD4). This research usin...

  2. Antioxidant activities of some local bangladeshi fruits (Artocarpus heterophyllus, Annona squamosa, Terminalia bellirica, Syzygium samarangense, Averrhoa carambola and Olea europa).

    Science.gov (United States)

    Soubir, Titov

    2007-03-01

    In the present study, antioxidant activities of the fruits of A. heterophyllus, A. squamosa, T. bellirica, S. samarangense, A. carambola and O. europa were investigated. For this, at first matured fruits of them were sliced into small pieces and dried in the sun and finally crushed in a grinder to make powder. Ethanolic extracts of fruit powder were prepared using 99.99% ethanol. The antioxidative activities of these extracts were determined according to their abilities of scavenging 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical. It was demonstrated that all the ethanolic extracts of A. heterophyllus, A. squamosa, T. bellirica, S. samarangense, A. caranbola and O. europa showed antioxidant activities. The IC50 of the ethanolic extracts of A. heterophyllus, A. squamosa, T. bellirica, S. samarangense, A. carambola and O. europa were 410, 250, 34, 200, 30 and 76 microg/mL, respectively. Among them, A. carambola showed the highest antioxidant activities followed by T. bellirica, O. europa, S. samarangense, A. squamosa and A. heterophyllus indicating that fruits of A. carambola, T. bellirica and O. europa are very beneficial to human health.

  3. Chemical Changes during the Fortification of Cassava Meal (Gari ...

    African Journals Online (AJOL)

    The nutritional enrichment of a cassava meal (gari) with African breadfruit seed residue was investigated. Grated cassava (70%) was fermented for 3 days with the incorporation of African breadfruit seed residue (30%) at different stages of the fermentation. The fortified and unfortified gari samples were subjected to ...

  4. Microbiological, proximate analysis and sensory evaluation of baked ...

    African Journals Online (AJOL)

    The possibility of making bread of good nutritional, microbiological and sensory qualities from blends of wheat-breadfruit flours was examined. Blends of wheat flour (WF) with percentages of 0, 5, 10, 15, 20 and 25 of breadfruits flour (BF) were used in the production process. The proximate analysis, sensory evaluation and ...

  5. Weight analyses and nitrogen balance assay in rats fed extruded ...

    African Journals Online (AJOL)

    Weight analyses and nitrogen balance assay in adult rats in raw and extruded African breadfruit (Treculia africana) based diets were carried out using response surface methodology in a central composite design. Process variables were feed composition (40 - 100 % African breadfruit, 0 - 5 % corn and 0 - 55 % soybean, ...

  6. 2018-04-27T07:42:46Z https://www.ajol.info/index.php/all/oai oai:ojs ...

    African Journals Online (AJOL)

    article/84776 2018-04-27T07:42:46Z jard:ART Nutritional Composition of the Flour of African Breadfruit (Treculia africana) Seeds Testa Adeyeye, EI Adesina, JA Chemical composition of the African breadfruit (Treculia africana) seeds testa was ...

  7. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Lans Cheryl A

    2006-10-01

    Full Text Available Abstract Background This paper is based on ethnobotanical interviews conducted from 1996–2000 in Trinidad and Tobago with thirty male and female respondents. Methods A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. Results The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Conclusion Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol

  8. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus

    Science.gov (United States)

    Lans, Cheryl A

    2006-01-01

    Background This paper is based on ethnobotanical interviews conducted from 1996–2000 in Trinidad and Tobago with thirty male and female respondents. Methods A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. Results The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Conclusion Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol. Eggplant extract as a

  9. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus.

    Science.gov (United States)

    Lans, Cheryl A

    2006-10-13

    This paper is based on ethnobotanical interviews conducted from 1996-2000 in Trinidad and Tobago with thirty male and female respondents. A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol. Eggplant extract as a hypocholesterolemic agent has some support but

  10. Production of bioplastic from jackfruit seed starch (Artocarpus heterophyllus) reinforced with microcrystalline cellulose from cocoa pod husk (Theobroma cacao L.) using glycerol as plasticizer

    Science.gov (United States)

    Lubis, M.; Gana, A.; Maysarah, S.; Ginting, M. H. S.; Harahap, M. B.

    2018-02-01

    The production of bioplastic from jackfruit seed starch reinforced with microcrystalline cellulose (MCC) cocoa pod husk using glycerol as plasticizer was investigated to determine the most optimum mass and volume of MCC and glycerol in producing bioplastics. To produce MCC, Cocoa pod husk was subjected to alkali treatment, bleaching, and hydrochloric acid hydrolysis. The degree of crystallinity of MCC, were determined by XRD, functional group by FT-IR and morphologycal analysis by SEM. Analysis of bioplastic mechanical properties includes tensile strength and elongation at break based on ASTM D882 standard. Bioplastics were produced by casting method from jackfruit seed starch and reinforced with MCC from cocoa pod husk at starch mass to MCC ratio of 6:4, 7:3, 8:2, and 9:1, using glycerol as plasticizer at 20%, 25%, 30% (wt/v of glycerol to starch). From the result, the isolated MCC from cocoa pod husk were in a form of rod-like shape of length 5-10 µm with diameter 11.635 nm and 74% crystallinity. The highest tensile strength of bioplastics was obtained at starch to MCC mass ratio of 8:2, addition of 20% glycerol with measured tensile strength of 0.637 MPa and elongation at break of 7.04%. Transform infrared spectroscopy showed the functional groups of bioplastics, which the majority of O-H groups were found at the bioplastics with reinforcing filler MCC that represented substantial hydrogen bonds.

  11. Utilization starch of jackfruit seed (Artocarpus heterophyllus) as raw material for bioplastics manufacturing using sorbitol as plasticizer and chitosan as filler

    Science.gov (United States)

    Lubis, M.; Harahap, M. B.; Manullang, A.; Alfarodo; Ginting, M. H. S.; Sartika, M.

    2017-01-01

    Starch is a natural polymer that can be used for the production of bioplastics because its source is abundant, renewable and easily degraded. Jackfruit seeds can be used as raw material for bioplastics because its contains starch. The aim of this study to determine the characteristics of jackfruit seeds and determine the effect of chitosan and sorbitol on the physicochemical properties of bioplastics from jackfruit seeds. Starch is extracted from jackfruit seeds were then characterized to determine its chemical composition. In the manufacture of bioplastics starch composition jackfruit seeds - chitosan used was 7: 3, 8: 2 and 9: 1 (g/g), while the concentration of sorbitol used was 20%, 25%, 30%, 35%, and 40% by weight dry ingredients. From the analysis of jackfruit seed starch obtained water content of 6.04%, ash content of 1.08%, the starch content of 70.22%, 16.39% amylose content, amylopectin content of 53.83%, 4.68% protein content, fat content 0.54%. The best conditions of starch bioplastics jackfruit seeds obtained at a ratio of starch: chitosan (w/w) = 8: 2 and the concentration of plasticizer sorbitol 25% with tensile strength 13.524 MPa. From the results of FT-IR analysis indicated an increase for the OH group and the group NH on bioplastics due to the addition of chitosan and sorbitol. The results of mechanical tests is further supported by analysis of scanning electron microscopy (SEM) showing jackfruit seed starch has a small granule size with the size of 7.6 μm and in bioplastics with chitosan filler and plasticizer sorbitol their fracture surface is smooth and slightly hollow compared bioplastics without fillers chitosan and plasticizer sorbitol.

  12. Research Paper ISSN 0189-6016©2007

    African Journals Online (AJOL)

    Prof. Adewunmi

    effect (69.4 %) at 240 hours. ... treatments at 72 h post administration are similar, there was continued reduction in sugar ... Chemical composition, functional properties and baking potential of. African breadfruit kernel and wheat flour blends.

  13. Effect of Traditional Processing Techniques on the Nutritional and ...

    African Journals Online (AJOL)

    Michael Horsfall

    Composition of African Bread-Fruit (Treculia africana) Seeds. *IFEOMA I IJEH .... located mainly in the seed coat (Kumar et al, 1979;. Singh ... development and control of some metabolic processes ... (1996). Regulation of selenoprotein gene.

  14. Invasão biológica de Artocarpus heterophyllus Lam. (Moraceae em um fragmento de Mata Atlântica no Nordeste do Brasil: impactos sobre a fitodiversidade e os solos dos sítios invadidos Biological invasion of Artocarpus heterophyllus Lam. (Moraceae in an Atlantic Forest fragment in Northeastern Brazil: impacts on phytodiversity and soils of invaded sites

    Directory of Open Access Journals (Sweden)

    Juliano Ricardo Fabricante

    2012-06-01

    Full Text Available O objetivo do trabalho foi avaliar os impactos causados por A. heterophyllus sobre a riqueza e diversidade da vegetação e sobre os solos de um fragmento de Floresta Ombrófila Aberta, Município de Areia, PB. A área estudada localiza-se no Campus II da Universidade Federal da Paraíba. No fragmento, foram instaladas 10 pares de parcelas de 100 m². Metade destas unidades amostrais (AI - ambiente invadido foi disposta de forma que apresentasse em seu centro um exemplar adulto de A. heterophyllus. Paralelamente a cada uma destas parcelas, outra unidade amostral (AN - ambiente natural foi alocada. Foi avaliado o número de espécies, a abundância de indivíduos, a diversidade e a equabilidade, além da química dos solos. Foram amostrados 14.008 indivíduos pertencentes a 79 espécies, dos quais 12.369 indivíduos de 50 espécies no AI e 1.639 indivíduos de 75 espécies no AN. A diversidade e a equabilidade geral foram, respectivamente, 0,24 e 0,06 no AI e 3,42 e 0,79 no AN. Todas as variáveis apresentaram diferenças significativas demonstrando que A. heterophyllus modifica os ambientes onde se dispersa. Quanto aos solos, alguns elementos tiveram suas concentrações mudadas enquanto outros não. Constatou-se que A. heterophylus altera de forma contundente a riqueza de espécies, a diversidade da vegetação e os solos dos sítios invadidos, o que torna necessárias ações de mitigação para o controle da mesma.The objective of this study was to evaluate the impacts caused by A. heterophyllus on the richness and diversity of vegetation, and the soils, in a fragment of open rainforest, in Areia, PB. The study area is located in the Campus II of Universidade Federal da Paraíba. In this fragment, 10 pairs of plots of 100 m² were established. Half of these sampling units (AI - environment invaded were laid out so there was an adult A. heterophyllus in the center. Alongside each of these plots, one sample unit (AN - natural environment was allocated. We evaluated the number of species, the abundance of individuals, diversity and evenness, and the chemistry of the soils. We sampled 14,008 individuals belonging to 79 species, of which there were 12,369 individuals and 50 species in the AI and 1,639 individuals and 75 species in the AN. The diversity and evenness of AI were 0.24 and 0.06, respectively, and for AN they were 3.42 and 0.79. All variables were significantly different showing that A. heterophyllus modify the environments where it grows. The concentrations of some elements in the soil changed while others did not. It was found that A. heterophylus forcefully alters species richness, diversity of vegetation and soils of invaded sites, which suggests that actions to control this species are necessary.

  15. Desidratação osmótica de frutículos de jaca (Artocarpus integrifolia L.: aplicação de modelos matemáticos = Osmotic dehydration of jackfruit (Artocarpus integrifolia L.: application of mathematical models

    Directory of Open Access Journals (Sweden)

    Tatiana Sant'Anna de Souza

    2009-04-01

    Full Text Available A desidratação osmótica tem sido sugerida como uma operação unitáriaimportante, na transformação de frutos perecíveis em novos produtos com maior vida de prateleira e maior valor agregado. A aplicação de modelos matemáticos confiáveis torna possível prever o comportamento de diversos fenômenos que ocorrem no processo, bem como possibilita a redução do seu custo operacional. Este trabalho teve como objetivo oestudo da aplicação de modelos matemáticos para as variáveis perda de água, ganho de sólidos, pH e ATT, durante a desidratação osmótica dos frutículos de jaca em solução de sacarose a 64°Brix, para diferentes temperaturas (40, 50, 60 e 70°C das soluções, no tempo de 2.880 min. O Modelo Decaimento Exponencial 2 se ajustou melhor aos dadosexperimentais de perda de água, constatando-se variação do coeficiente de determinação de 76,73 a 97,16%, enquanto para as variáveis SST e ATT, o Modelo Logístico ajustou-se melhor aos dados experimentais, obtendo variação de 80,5 a 98,2% e 89,5 a 99,4%. Já para o pH, o Modelo Decaimento Exponencial 1 obteve bom ajuste aos dados experimentais, com coeficientes de determinação variando de 80,5 a 98,2%.Osmotic dehydration has been suggested as an important unitary operation in the processing of perishable fruit into new products withgreater shelf live and aggregate value. The application of reliable mathematical models makes it possible to predict the behavior of several phenomena that occur in the process, enabling the reduction of operational cost as well. The objective of this paper was to studythe application of mathematical models to water loss, solid gain, pH and titratable acidity (TA variables during osmotic dehydration of jackfruit in sucrose solution at 64ºbrix and at different concentrations of sucrose and different solution temperatures (40, 50, 60 and 70ºC, for 2,880 minutes. The exponential decay model 2 was better adjusted to water loss experimental data, with the determination coefficient ranging from 76.73% to 97.16%,whereas for the variables total soluble solids (TSS and TA, the logistic model was better adjusted to the experimental data, obtaining a variation from 80.5 to 98.2% and from 89.5 to99.4%. For pH, the exponential decay model 1 obtained a good adjustment to the experimental data, with determination coefficients ranging from 80.5 to 98.2%.

  16. Productividad de una plantación de espárrago verde (Asparagus officinalis var. altilis L. con diferentes tamaños de “arañas” y densidades, en su séptimo año

    Directory of Open Access Journals (Sweden)

    J. Marina

    2012-01-01

    Full Text Available El espárrago es un cultivo rústico que tradicionalmente se ha iniciado mediante el sistema tradicional de “arañas”. El presente ensayo tuvo como objetivo evaluar la productividad total y comercial de una plantación de espárrago verde al séptimo año desde la plantación. El ensayo de 1.728 m2 está ubicado en la Chacra Experimental de la Facultad de Agronomía de la UNCPBA (36º 48’ latitud Sur y 59º 51’ longitud Occidental, en Azul, Argentina. La plantación, iniciada el 12/09/2003, se evaluó en el período 17/09/2010-01/11/2010. Las variables estudiadas fueron: tamaños de “arañas” grandes (AG > 200 g, medianas (AM 100-200 g y pequeñas (AP 0,05. El rendimiento general del ensayo fue PFT: 19.758 y PFN: 10.018 kg/ha; mientras que NTT: 483.425 y NTC: 477.200. En PFT se observaron diferencias significativas (p 0,05, siendo la media en PFN: 9.910 kg/ha. En densidad y en tamaño de “arañas” los resultados difirieron de los encontrados 2 años anteriores en los que se destacaron mayor densidad y tamaño grande; efectos atribuibles a la competencia intraespecífica.

  17. Productividad de una plantación de espárrago verde (Asparagus officinalis var. altilis L. con diferentes tamaños de “arañas” y densidades, en su séptimo año

    Directory of Open Access Journals (Sweden)

    A. M. Castagnino

    2012-12-01

    Full Text Available El espárrago es un cultivo rústico que tradicionalmente se ha iniciado mediante el sistema tradicional de “arañas”. El presente ensayo tuvo como objetivo evaluar la productividad total y comercial de una plantación de espárrago verde al séptimo año desde la plantación. El ensayo de 1.728 m2 está ubicado en la Chacra Experimental de la Facultad de Agronomía de la UNCPBA (36º 48’ latitud Sur y 59º 51’ longitud Occidental, en Azul, Argentina. La plantación, iniciada el 12/09/2003, se evaluó en el período 17/09/2010-01/11/2010. Las variables estudiadas fueron: tamaños de “arañas” grandes (AG > 200 g, medianas (AM 100-200 g y pequeñas (AP 0,05. El rendimiento general del ensayo fue PFT: 19.758 y PFN: 10.018 kg/ha; mientras que NTT: 483.425 y NTC: 477.200. En PFT se observaron diferencias significativas (p 0,05, siendo la media en PFN: 9.910 kg/ha. En densidad y en tamaño de “arañas” los resultados difirieron de los encontrados 2 años anteriores en los que se destacaron mayor densidad y tamaño grande; efectos atribuibles a la competencia intraespecífica.

  18. Evaluating coagulant activity of locally available Syzygium cumini ...

    African Journals Online (AJOL)

    ... where it was: alum>Artocarpus heterophyllus>Moringa oleifera >Syzygium cumini.Optimal turbidity removal was: Moringa oleifera 97.7%, Artocarpus heterophyllus 95.8% and Syzygium cumini 94.1%. The seed extracts were also effective as water disinfectants (Moringa>Jackfruit>Java plum). The use of Jackfruit and Java ...

  19. Screening of traditional antidiabetic medicinal plants of Mauritius for possible alpha-amylase inhibitory effects in vitro.

    Science.gov (United States)

    Kotowaroo, M I; Mahomoodally, M F; Gurib-Fakim, A; Subratty, A H

    2006-03-01

    In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (Poaceae), Aegle marmelos (Rutaceae), Artocarpus heterophyllus (Moraceae), Vangueria madagascariensis (Rubiaceae), Azadirachta indica (Meliaceae), Eriobotrya japonica (Rosaceae) and Syzigium cumini (Myrtaceae) were studied for possible effects on starch breakdown by alpha-amylase in vitro. The results showed that only Artocarpus heterophyllus significantly (p Artocarpus heterophyllus on alpha-amylase activity using rat plasma in vitro. It was found that the aqueous leaf extract significantly (p Artocarpus heterophyllus behaved as a competitive inhibitor. Results from the present study tend to indicate that Artocarpus heterophyllus could act as a 'starch blocker' thereby reducing post-prandial glucose peaks. Copyright 2006 John Wiley & Sons, Ltd.

  20. Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection.

    Science.gov (United States)

    Ramos, Eliézer L P; Santana, Silas S; Silva, Murilo V; Santiago, Fernanda M; Mineo, Tiago W P; Mineo, José R

    2016-01-01

    Infection by Toxoplasma gondii affects around one-third of world population and the treatment for patients presenting toxoplasmosis clinically manifested disease is mainly based by a combination of sulfadiazine, pyrimethamine, and folinic acid. However, this therapeutic protocol is significantly toxic, causing relevant dose-related bone marrow damage. Thus, it is necessary to improve new approaches to investigate the usefulness of more effective and non-toxic agents for treatment of patients with toxoplasmosis. It has been described that lectins from plants can control parasite infections, when used as immunological adjuvants in vaccination procedures. This type of lectins, such as ArtinM and ScLL is able to induce immunostimulatory activities, including efficient immune response against parasites. The present study aimed to evaluate the potential immunostimulatory effect of ScLL and ArtinM for treatment of T. gondii infection during acute phase, considering that there is no study in the literature accomplishing this issue. For this purpose, bone marrow-derived macrophages (BMDMs) were treated with different concentrations from each lectin to determine the maximum concentration without or with lowest cytotoxic effect. After, it was also measured the cytokine levels produced by these cells when stimulated by the selected concentrations of lectins. We found that ScLL showed high capacity to induce of pro-inflammatory cytokine production, while ArtinM was able to induce especially an anti-inflammatory cytokines production. Furthermore, both lectins were able to increase NO levels. Next, we evaluated the treatment effect of ScLL and ArtinM in C57BL/6 mice infected by ME49 strain from T. gondii . The animals were infected and treated with ScLL, ArtinM, ArtinM plus ScLL, or sulfadiazine, and the following parameters analyzed: Cytokines production, brain parasite burden and survival rates. Our results demonstrated that the ScLL or ScLL plus ArtinM treatment induced production of pro-inflammatory and anti-inflammatory cytokines, showing differential but complementary profiles. Moreover, when compared with non-treated mice, the parasite burden was significantly lower and survival rates higher in mice treated with ScLL or ScLL plus ArtinM, similarly with sulfadiazine treatment. In conclusion, the results demonstrated the suitable potential immunotherapeutic effect of ScLL and ArtinM lectins to control acute toxoplasmosis in this experimental murine model.

  1. Lectins from Synadenium carinatum (ScLL and Artocarpus heterophyllus (ArtinM are able to induce beneficial immunomodulatory effects in a murine model for treatment of Toxoplasma gondii infection

    Directory of Open Access Journals (Sweden)

    Leandro Peixoto Ferreira Souza

    2016-11-01

    Full Text Available Infection by Toxoplasma gondii affects around one-third of world population and the treatment for patients presenting toxoplasmosis clinically manifested disease is mainly based by a combination of sulfadiazine, pyrimethamine and folinic acid. However, this therapeutic protocol is significantly toxic, causing relevant dose-related bone marrow damage. Thus, it is necessary to improve new approaches to investigate the usefulness of more effective and non-toxic agents for treatment of patients with toxoplasmosis. It has been described that lectins from plants can control parasite infections, when used as immunological adjuvants in vaccination procedures. This type of lectins, such as ArtinM and ScLL is able to induce immunostimulatory activities, including efficient immune response against parasites. The present study aimed to evaluate the potential immunostimulatory effect of ScLL and ArtinM for treatment of T. gondii infection during acute phase, considering that there is no study in the literature accomplishing this issue. For this purpose, bone marrow-derived macrophages (BMDMs were treated with different concentrations from each lectin to determine the maximum concentration without or with lowest cytotoxic effect. After, it was also measured the cytokine levels produced by these cells when stimulated by the selected concentrations of lectins. We found that ScLL showed high capacity to induce of pro-inflammatory cytokine production, while ArtinM was able to induce especially an anti-inflammatory cytokines production. Furthermore, both lectins were able to increase NO levels. Next, we evaluated the treatment effect of ScLL and ArtinM in C57BL/6 mice infected by ME49 strain from T. gondii. The animals were infected and treated with ScLL, ArtinM, ArtinM plus ScLL or sulfadiazine, and the following parameters analyzed: cytokines production, brain parasite burden and survival rates. Our results demonstrated that the ScLL or ScLL plus ArtinM treatment induced production of pro-inflammatory and anti-inflammatory cytokines, showing differential but complementary profiles. Moreover, when compared with non treated mice, the parasite burden was significantly lower and survival rates higher in mice treated with ScLL or ScLL plus ArtinM, similarly with sulfadiazine treatment. In conclusion, the results demonstrated the suitable potential immunotherapeutic effect of ScLL and ArtinM lectins to control acute toxoplasmosis in this experimental murine model.

  2. Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke

    NARCIS (Netherlands)

    Acquadro, A.; Lanteri, S.; Scaglione, D.; Arens, P.F.P.; Vosman, B.; Portis, E.

    2009-01-01

    Cynara cardunculus includes three taxa, the globe artichoke (subsp. scolymus L. Hegi), the cultivated cardoon (var. altilis) and their progenitor, the wild cardoon (var. sylvestris). Globe artichoke is an important component of the Mediterranean rural economy, but its improvement through breeding

  3. Maire Valtin : meetootmine pole meelakkumine / Liisi Kanna

    Index Scriptorium Estoniae

    Kanna, Liisi

    2015-01-01

    Vestlusest Lääne-Virumaal Inju külas mesindusega tegeleva Maire Valtiniga. Valtinite perefirma Artocarpus on välja töötanud palju ravimtaimede ja mesindussaadustega meesegusid. Pidevalt on kaubavalikus umbes 15 toodet

  4. Variation in biofouling on different species of Indian timbers

    Digital Repository Service at National Institute of Oceanography (India)

    Raveendran, T.V.; Wagh, A.B.

    Biofouling on twenty species of wood exposed in waters of Mormugao Harbour, Goa, India have been presented. Macrofouling biomass varied from species to species. Maximum biomass was recorded on Artocarpus chaplasha (4 kg/m2) and minimum on Hopea...

  5. The systematic wood anatomy of the Moraceae (Uriticales). V. Genera of the tribe Moreae without urticaceous stamens

    NARCIS (Netherlands)

    Welle, ter B.J.H.; Koek-Noorman, J.; Topper, S.M.C.

    1983-01-01

    The wood anatomy of the Moreae without urticaceous stamens is described in detail. Generic descriptions of the following genera are provided: Antiaropsis, Artocarpus, Bagassa, Batocarpus, Clarisia, Parartocarpus, Poulsenia, Prainea, Sorocea, Sparattosyce, and Treculia. Wood anatomical variation

  6. Surface sterilization and duration of seed storage influenced ...

    African Journals Online (AJOL)

    The effects of factorial combinations of four storage duration (in days after seed extraction) and surface sterilization with three dilution levels of sodium hypochlorite on seedling emergence and seedling quality of African breadfruit were studied. Storage duration significantly influenced days to seedling emergence, ...

  7. Kinetics of saponification of Treculia africana oil using a locally ...

    African Journals Online (AJOL)

    The investigation on the saponification of Treculia africana (breadfruit) oil using a locally obtained alkaline (Ngu) extracted from the burnt female inflorescence of oil palm bunch using kinetic approach proved successful and showed a high probability of producing good quality toilet soap. The result obtained revealed that ...

  8. Production and evaluation of noodles from flour blends of cocoyam ...

    African Journals Online (AJOL)

    Flour blends were prepared from two cultivars of cocoyam ( and . ), African breadfruit ( ) and wheat ( ) at different proportions and extruded into noodles with a locally fabricated and manually operated singlescrew extruder. Proximate composition and the functional properties of the different flour blends were evaluated. The

  9. Browse Title Index

    African Journals Online (AJOL)

    Items 51 - 100 of 263 ... Vol 5, No 2 (2006), Blood glucose level and lipid profile in rats fed on Treculia Africana (Breadfruit) diet: A sub-chronic study, Abstract. OO Okwari, OE Ofem, ... Vol 9, No 2 (2010), Comparative Effect of Fresh, Thermoxidized and Irradiated Oil on Gastric Acid Secretion and Cytoprotection in Rats, Abstract.

  10. Journal of Research in Forestry, Wildlife and Environment - Vol 8 ...

    African Journals Online (AJOL)

    Socio-economic contribution of African breadfruit (Treculia africana Decne) toward sustainable livelihood in eastern, Nigeria · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. O.B. Uluocha, A.U. Udeagha, S.I. Udofia, C.I. Duruigbo, 40-57 ...

  11. Nutrient digestibility and nitrogen metabolism in West African dwarf ...

    African Journals Online (AJOL)

    An experiment was conducted to determine nutrient digestibility and nitrogen metabolism in sheep fed differently processed breadfruit meal. Twenty West African dwarf sheep with mean weight body of 6.00 ± 0.55kg were randomly allotted to four dietary treatments with five sheep per treatment in a completely randomised ...

  12. Final integrated trip report: site visits to Area 50, Andersen Air Force Base, Guam National Wildlife Refuge, War in the Pacific National Historical Park, Guam, Rota and Saipan, CNMI, 2004-2005

    Science.gov (United States)

    Hess, Steven C.; Pratt, Linda W.

    2006-01-01

    supported the Mariana Gallinule (Gallinula chloropus guami), the Mariana Mallard (Anas platyrhynchos oustaleti), Mariana Fruit-Dove (Ptilinopus roseicapilla), White-throated Ground Dove (Gallicolumba xanthonura xanthonura), Mariana Crow (Corvus kubaryi), and the Nightingale Reed-warbler (Acrocephalus luscinia), all endemic to the Mariana Islands. Other regionally endemic endangered species include the Micronesian Megapode (Megapodius laperouse), and the Mariana Swiftlet (Aerodramus bartschi), now reduced to a small population on Guam. Likewise, the flora of Guam is unique, with 21percent of its native vascular plants endemic to the Mariana Islands. In limestone forests of Northern Guam, a number of tall forest tree species such as joga, Elaeocarpus joga (Elaeocarpaceae); pengua or Macaranga thompsonii (Euphorbiaceae); ifit or Intsia bijuga (Fabaceae); seeded breadfruit or Artocarpus marianensis (Moraceae); and umumu or Pisonia grandis (Nyctaginaceae) may be in decline as a result of herbivory by mammals. All show reduced regeneration and age distributions highly skewed towards older individuals. These species provided important habitat for some of Guam's endangered forest birds that remain in captivity such as the Mariana Crow, Guam Kingfisher, and Guam Rail. The recent high frequency of intense tropical storms and herbivory caused by large populations of feral pigs and Philippine sambar deer (Cervus mariannus), as well as invasive alien vines that may suppress tree regeneration, could be permanently altering the structure of regenerating forests and composition of important canopy species on secondary limestone substrates that were cleared and compacted during airfield construction from 1944 through the 1970s. Guam National Wildlife Refuge (GNWR) was established at Ritidian Point, after it was determined to be excess property by the U.S. Navy. Most of the refuge, about 9,087 hectares, is an 'overlay refuge' on lands administered by the U.S. Air Force and U.S. Navy

  13. Breast Cancer Epidemiology in Puerto Rico

    Science.gov (United States)

    2008-06-30

    consumed in the past , this is during the last 12 months. (The interviewer can help by providing exact date from this month, back to a year ago: for...Whole wheat bread White bread Yellow tubers: yam, pumpkin , squash Other: potato, plantain, taro root, tanier root, breadfruit...your regular diet as reported earlier, in the past two years? _____ Yes and the changes are the following (1) ______No (2

  14. Migan

    Directory of Open Access Journals (Sweden)

    Richard Price

    1994-01-01

    Full Text Available [First paragraph] Our friend Charlemagne (a.k.a. Émilien, who lives down the road and considers himself a breadfruit connoisseur, says that there's only one other tree in southern Martinique whose fruit compares with ours. From our back porch, during the tree's several flowerings each year, we can reach out and piek low-growing fruit by hand, or with a knife-and-pole contraption cut down a milk-flecked orb from higher up in the broad green leaves. This particular tree may even be descended from the oldest breadfruit in the Caribbean, for Martinique was already blessed with trees, transported from "L'ïle-de-France" (Mauritius, by the time Captain Bligh made his 1791-93 voyage from Polynesia, "bringing breadfruit from what was seen to be a Tree of Life in the islands of Paradise ... the very symbol of a free and unencumbered life ... to feed slaves, the living dead of the Caribbean" (Dening 1992:4, 11.

  15. Desidratação osmótica de frutículos de jaca (Artocarpus integrifólia L.: aplicação de modelos matemáticos - DOI: 10.4025/actascitechnol.v31i2.1026

    Directory of Open Access Journals (Sweden)

    Tatiana Sant'Anna de Souza

    2009-06-01

    Full Text Available A desidratação osmótica tem sido sugerida como uma operação unitária importante, na transformação de frutos perecíveis em novos produtos com maior vida de prateleira e maior valor agregado. A aplicação de modelos matemáticos confiáveis torna possível prever o comportamento de diversos fenômenos que ocorrem no processo, bem como possibilita a redução do seu custo operacional. Este trabalho teve como objetivo o estudo da aplicação de modelos matemáticos para as variáveis perda de água, ganho de sólidos, pH e ATT, durante a desidratação osmótica dos frutículos de jaca em solução de sacarose a 64°Brix, para diferentes temperaturas (40, 50, 60 e 70°C das soluções, no tempo de 2.880 min. O Modelo Decaimento Exponencial 2 se ajustou melhor aos dados experimentais de perda de água, constatando-se variação do coeficiente de determinação de 76,73 a 97,16%, enquanto para as variáveis SST e ATT, o Modelo Logístico ajustou-se melhor aos dados experimentais, obtendo variação de 80,5 a 98,2% e 89,5 a 99,4%. Já para o pH, o Modelo Decaimento Exponencial 1 obteve bom ajuste aos dados experimentais, com coeficientes de determinação variando de 80,5 a 98,2%.

  16. Carbon sequestration rate and aboveground biomass carbon potential of three young species in lower Gangetic plain.

    Science.gov (United States)

    Jana, Bipal K; Biswas, Soumyajit; Majumder, Mrinmoy; Roy, Pankaj K; Mazumdar, Asis

    2011-07-01

    Carbon is sequestered by the plant photosynthesis and stored as biomass in different parts of the tree. Carbon sequestration rate has been measured for young species (6 years age) of Shorea robusta at Chadra forest in Paschim Medinipur district, Albizzia lebbek in Indian Botanic Garden in Howrah district and Artocarpus integrifolia at Banobitan within Kolkata in the lower Gangetic plain of West Bengal in India by Automated Vaisala Made Instrument GMP343 and aboveground biomass carbon has been analyzed by CHN analyzer. The specific objective of this paper is to measure carbon sequestration rate and aboveground biomass carbon potential of three young species of Shorea robusta, Albizzia lebbek and Artocarpus integrifolia. The carbon sequestration rate (mean) from the ambient air during winter season as obtained by Shorea robusta, Albizzia lebbek and Artocarpus integrifolia were 11.13 g/h, 14.86 g/h and 4.22g/h, respectively. The annual carbon sequestration rate from ambient air were estimated at 8.97 t C ha(-1) by Shorea robusta, 11.97 t C ha(-1) by Albizzia lebbek and 3.33 t C ha(-1) by Artocarpus integrifolia. The percentage of carbon content (except root) in the aboveground biomass of Shorea robusta, Albizzia lebbek and Artocarpus integrifolia were 47.45, 47.12 and 43.33, respectively. The total aboveground biomass carbon stock per hectare as estimated for Shorea robusta, Albizzia lebbek and Artocarpus integrifolia were 5.22 t C ha(-1) , 6.26 t C ha(-1) and 7.28 t C ha(-1), respectively in these forest stands.

  17. A new species of Klabonosa Bouček (Hymenoptera: Pteromalidae) reared from eggs of Endochus sp. (Hemiptera: Reduviidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Yeshwanth, H M; Sureshan, P M

    2018-04-23

    The genus Klabonosa Bouček (Hymenoptera: Pteromalidae) is recorded for the first time from the Oriental region, with both sexes of K. indica Gupta, Sureshan Yeshwanth sp. n. reared from eggs of the assassin bug Endochus sp. (Hemiptera: Reduviidae) on Artocarpus heterophyllus Lam. (Moraceae). The male is formally described and illustrated for the first time for the genus.

  18. PENGOLAHAN CISTIK SUKUN DI DESA MERTASINGA KECAMATAN CILACAP UTARA

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    Tri Watiningsih

    2017-03-01

    Full Text Available ABSTRAK Tujuan dari kegiatan ini adalah: 1 Memberikan pengetahuan teoritis kepada Produsen Cistik Sukun di Desa Mertasinga dan Desa Tritih Wetan Kecamatan Cilacap Utara tentang bagaimana membuat dan mengemas hasil produksi yang baik 2 Meningkatan Mutu hasil olahan sukun di Desa Mertasingan dan Desa Tritih Wetan Kecamatan Cilacap Utara untuk mengolah sukun dengan sentuhan teknologi dan Sains 3 Memperkuat produsen cistik sukundi Desa Mertasingan dan Desa Tritih Wetan Kecamatan Cilacap Utaramenjadi masyarakat yang mandiri secara ekonomi.Kegiatan ini terdiri dari pemberian pengetahuan tentang pengolahan sukun agar lebih menarik dan mendapatkan nilai jual yang baik, pelatihan pembuatan cistik sukun dan pengemasan, pelatihan pembuatan anggaran dalam penjualan,evaluasi, pameran hasildan pendampingan. Sedangkan metode yang digunakan dalam kegiatan ini dalah ceramah, pemberian pelatihan, demonstrasi, simulasi pembuatan cistik sukun.Target luaran kegiatan ini berupa:1 Media pembelajaran pengetahuan bagi produsen cistik sukun, 2. Pengetahuan tentang pengemasan cistik sukun agar lebih menarik dan mendapatakan harga jual yang tinggi,3. Bermanfaat bagi masyarakat khususnya produsen cistik sukun menjadi masyarakat yang mandiri secara ekonomi. Keyward :Sukun, Pengolahan, Cistik, Pengemasan. Pemasaran Sukun ABSTRACT The purposeof this activity are: 1 To provide theoretical knowledge to the “Cistik” Breadfruit producer in Mertasinga and Tritih Wetan vistage, North Cilacap sub district in foodprocessing and packaging 2 To increase the quality ofbreadfruit in Mertasingan and Tritih Wetan village by using technology and Science approahe3 Cistik breacfruit producent stregtring in Mertasingan and Tritih Wetan vistage, Nort Cilacap Utara Sub distrik to be economically self sufficient communities .This activities are consistan of processing supervision, packaging, food production training,budgeting, produc tevaluation, exhibitions hasildan assistance. While the

  19. POTENTIAL OF BREAD FRUIT- AN ECOLOGICALLY FOREST BASED FEED RESOURCE IN RABBIT NUTRITION

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    Oso Abimbola Oladele

    2009-09-01

    Full Text Available  Productive response of rabbits fed with diets containing graded levels of sun dried breadfruit (SBFM meal were studied using 80 unsexed weaner rabbits. Four dietary treatments were formulated such that SBFM replaced maize weight for weight at 0%, 22.2%, 44.4% and 66.6% levels respectively. Rabbits were randomly allotted to the dietary treatments such that there were 20 rabbits per treatment. Growth response, nutrient utilization, haematological indices, serum biochemistry, carcass yield and meat composition were evaluated. Rabbits fed with 22.2% maize replaced with SBFM recorded the highest (P

  20. Migan

    OpenAIRE

    Richard Price; Sally Price

    1994-01-01

    [First paragraph] Our friend Charlemagne (a.k.a. Émilien), who lives down the road and considers himself a breadfruit connoisseur, says that there's only one other tree in southern Martinique whose fruit compares with ours. From our back porch, during the tree's several flowerings each year, we can reach out and piek low-growing fruit by hand, or with a knife-and-pole contraption cut down a milk-flecked orb from higher up in the broad green leaves. This particular tree may even be descen...

  1. Food resources, distribution and seasonal variations in ranging in lion-tailed macaques, Macaca silenus in the Western Ghats, India.

    Science.gov (United States)

    Erinjery, Joseph J; Kavana, T S; Singh, Mewa

    2015-01-01

    The distribution and availability of food was examined to see how it influenced ranging patterns and sleeping site selection in a group of lion-tailed macaques. The home range and core area were 130.48 ha (95% kernel) and 26.68 ha (50% kernel) respectively. The lion-tailed macaques had a longer day range, had a greater number of sleeping sites and used more core areas in the summer as compared to the monsoon and the post-monsoon seasons. The ranging patterns and sleeping site use were influenced by the major food resources used in a particular season. The ranging was mainly influenced by Artocarpus heterophyllus in monsoon, Cullenia exarillata and Toona ciliata in post- monsoon, and Artocarpus heterophyllus and Ficus amplissima in summer. The distribution of these four plant species is, therefore, critical to ranging, and thus to conservation of the lion-tailed macaque.

  2. ASOSIASI AKAR KUNING (Fibraurea tinctoria Lour. DENGAN TUMBUHAN BERPOTENSI OBAT DI SAMBOJA, KALIMANTAN TIMUR

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    Noorcahyati Noorcahyati

    2017-06-01

    Full Text Available Akar kuning (Fibraurea tinctoria Lour. Is a medicinal plant at Kalimantan that has the potential of development and require conservation efforts. This research was aimed to observe the ecology, association and other plants that have the potential as medicinal plants in the community garden in Sungai Merdeka, Samboja East Kalimantan. Data collection were using purposive sampling plot of 20 x 20 m. Found 28 species of 21 families among the sites of various stages of growth. The highest IVI value at every growth is Fibraurea tinctoria with an IVI of 78.24 (seedlings, Archidendron jiringa with IVI 35.20 (saplings, Glochidion sp. IVI 109.94 (poles and Artocarpus integer IVIs 109.94 (trees. The strongest associations at the level of the tree is Artocarpus integer. At the root of the akar kuning habitat obtained 19 species of plants that also have potential as a drug that can be developed with agroforestry system. Akar kuning (Fibraurea tinctoria merupakan biofarmaka Kalimantan yang perlu mendapat perhatian untuk dikembangkan dan dilestarikan. Penelitian bertujuan untuk mendapatkan informasi ekologi, asosiasi dan tumbuhan lain yang berpotensi sebagai obat pada habitat akar kuning di kebun masyarakat di Desa Sungai Merdeka, Samboja Kalimantan Timur. Pengumpulan data dilakukan secara sengaja dengan teknik sampling kuadrat ukuran petak 20 x 20 m. Ditemukan 28 jenis dari 21 famili pada lokasi penelitian dari berbagai tingkat pertumbuhan. Nilai INP tertinggi pada setiap pertumbuhan adalah Fibraurea tinctoria dengan INP sebesar 78,24 (tingkat semai, jenis Archidendron jiringa dengan INP 35,20 (tingkat pancang, Glochidion sp. INP 109,94 (tingkat tiang dan Artocarpus integer memiliki INP 109,94 (tingkat pohon. Asosiasi terkuat pada tingkat pohon adalah Artocarpus integer. Pada habitat akar kuning diperoleh 19 jenis tumbuhan yang juga memiliki potensi sebagai obat yang dapat dikembangkan secara bersama-sama.

  3. Antioxidant Properties of Seeds from Lines of Artichoke, Cultivated Cardoon and Wild Cardoon

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    Alessandra Durazzo

    2013-06-01

    Full Text Available The artichoke (Cynara cardunculus L. subsp. scolymus L., the cultivated cardoon (Cynara cardunculus var. altilis DC. and the wild cardoon (Cynara cardunculus var. sylvestris L. are species widely distributed in the Mediterranean area. The aim of this research was to evaluate the antioxidant properties of seeds from lines of artichoke and cultivated and wild cardoon in both aqueous-organic extracts and their residues by FRAP (Ferric Reducing Antioxidant Power and TEAC (Trolox Equivalent Antioxidant Capacity evaluations. Both artichoke and cardoon seeds are a good source of antioxidants. Among artichoke seeds, hydrolysable polyphenols contribution to antioxidant properties ranged from 41% to 78% for FRAP values and from 17% to 37% for TEAC values. No difference between cultivated and wild cardoon in antioxidant properties are reported. Our results could provide information about the potential industrial use and application of artichoke and/or cardoon seeds.

  4. Antioxidant Properties of Seeds from Lines of Artichoke, Cultivated Cardoon and Wild Cardoon

    Science.gov (United States)

    Durazzo, Alessandra; Foddai, Maria Stella; Temperini, Andrea; Azzini, Elena; Venneria, Eugenia; Lucarini, Massimo; Finotti, Enrico; Maiani, Gianluca; Crinò, Paola; Saccardo, Francesco; Maiani, Giuseppe

    2013-01-01

    The artichoke (Cynara cardunculus L. subsp. scolymus L.), the cultivated cardoon (Cynara cardunculus var. altilis DC.) and the wild cardoon (Cynara cardunculus var. sylvestris L.) are species widely distributed in the Mediterranean area. The aim of this research was to evaluate the antioxidant properties of seeds from lines of artichoke and cultivated and wild cardoon in both aqueous-organic extracts and their residues by FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity) evaluations. Both artichoke and cardoon seeds are a good source of antioxidants. Among artichoke seeds, hydrolysable polyphenols contribution to antioxidant properties ranged from 41% to 78% for FRAP values and from 17% to 37% for TEAC values. No difference between cultivated and wild cardoon in antioxidant properties are reported. Our results could provide information about the potential industrial use and application of artichoke and/or cardoon seeds. PMID:26787623

  5. STUDI POTENSI PERTANIAN “KAKI“ LAWU SELATAN WILAYAH WONOGIRI

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    Arief Budi Nugroho

    2014-03-01

    Full Text Available Agricultural potential have the highest contribution to Gross Domestic Product Wonogiri (BPS, 2011 because it is proper database agricultural potential is necessary for basic agricultural development planning in particular and the economy in general. In turn can be achieved high revenue. This study aims to provide information on the potential of agriculture in the "Kaki Lawu Selatan" South and provide recommendations on the development of agricultural. From this study the South Lawu Foot Zone can be divided into 4 (four zones: the upper leg, foot zone central zone, lower leg and foot slope zone. Among these zones, based on the parent material foot zone less potential for commercial agriculture, based on the value of LQ, three priority commodities suspected potential for the avocado fruit, rambutan, breadfruit. Vegetables garlic, carrots and cabbage. Plantation commodities include coffee, cocoa and tobacco / patchouli. Food commodities include cassava, maize and peanuts.Kata kunci : Agricultural potential

  6. Analisis Nilai Tambah dan Usaha Pengolahan Tepung Sukun Sebagai Upaya Peningkatan Pendapatan Petani

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    Waryat Waryat

    2016-12-01

    Full Text Available Bread fruit processing into flour is an alternative way of processing which is has several advantages including; increase the shelf life, facilitate the process of raw materials and increase the selling price, while its nutrient is relatively unchanged. The purpose of this study is to examine the amount of value added product generated by bread fruit flour and to determine business analysis of bread fruit flour in Kepulauan Seribu, DKI Jakarta Province. Data is collected using interviews, observation and mutilation. Measurement is done by calculating the value added products resulting from the processing of bread fruit into bread fruit flour, while analysis of feasibility is calculated based on the analysis of profit and analysis of revenue and costs as well as the R/C ratio. Breadfruit flour business has gave product value added, profit and R/C ratio account Rp. 5,500 per kilogram, Rp. 218,000 and 1.57 respectively.

  7. New coating material for producing virgin coconut oil (VCO microcapsules

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    Amin, Z.A.

    2017-01-01

    Full Text Available The aim of this work was to investigate the microencapsulation efficiency (MEE of different grades of broken rice (RB and breadfruit (BB-based maltodextrin as a coating material, using virgin coconut oil (VCO as a model system. The VCO was generally found to be well microencapsulated using BB, RB or commercial (COM maltodextrin at a core/wall material ratio of 1:3. In comparison to a different dextrose equivalent (DE group, both RB and BB maltodextrins with DE values of 10-14 showed higher MEE values (84.81-94.39% than maltodextrins with DE value of 15-19 (78.23-79.65%. Low DE value maltodextrins were shown higher glass transition temperatures than high DE value maltodextrins under the same moisture content. Both RB and BB maltodextrins were found to be compatible with COM maltodextrin as shown in the microstructure appearance when viewed with a scanning electron microscope (SEM.

  8. Attraction and consumption of methyl eugenol by male Bactrocera umbrosa Fabricius (Diptera: Tephritidae) promotes conspecific sexual communication and mating performance.

    Science.gov (United States)

    Wee, S L; Abdul Munir, M Z; Hee, A K W

    2018-02-01

    The Artocarpus fruit fly, Bactrocera umbrosa (Fabricius) (Diptera: Tephritidae), is an oligophagous fruit pest infesting Moraceae fruits, including jackfruit (Artocarpus heterophyllus Lamarck), a fruit commodity of high value in Malaysia. The scarcity of fundamental biological, physiological and ecological information on this pest, particularly in relation to behavioural response to phytochemical lures, which are instrumental to the success of many area-wide fruit fly control and management programmes, underpins the need for studies on this much-underrated pest. The positive response of B. umbrosa males to methyl eugenol (ME), a highly potent phytochemical lure, which attracts mainly males of many Bactrocera species, was shown to increase with increasing age. As early as 7 days after emergence (DAE), ca. 22% of males had responded to ME and over 50% by 10 DAE, despite no occurrence of matings (i.e. the males were still sexually immature). Male attraction to ME peaked from 10 to 27 DAE, which corresponded with the flies' attainment of sexual maturity. In wind-tunnel assays during the dusk courtship period, ME-fed males exhibited earlier calling activity and attracted a significantly higher percentage of virgin females compared with ME-deprived males. ME-fed males enjoyed a higher mating success than ME-deprived males at 1-day post ME feeding in semi-field assays. ME consumption also promotes aggregation behaviour in B. umbrosa males, as demonstrated in wind-tunnel and semi-field assays. We suggest that ME plays a prominent role in promoting sexual communication and enhancing mating performance of the Artocarpus fruit fly, a finding that is congruent with previous reports on the consequences of ME acquisition by other economically important Bactrocera species.

  9. Extraction process optimization of polyphenols from Indian Citrus sinensis - as novel antiglycative agents in the management of diabetes mellitus.

    Science.gov (United States)

    Shakthi Deve, Asaithambi; Sathish Kumar, Thiyagarajan; Kumaresan, Kuppamuthu; Rapheal, Vinohar Stephen

    2014-01-07

    Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. It has become an epidemic disease in the 21st century where, India leads the world with largest number of diabetic subjects. Non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (AGEs) that leads to the other complicated secondary disorders. In this context, Mangifera indica (Mango), Syzygium cumini (Jambul), Vitis vinifera (Grapes), Citrus sinensis (Orange), Artocarpus heterophyllus (Jackfruit), Manilkara zapota (Sapodilla) seeds were evaluated for their antiglyation activity. Attempts were made to isolate the polyphenols in the seeds that have recorded the maximum activity. Different extraction methods (shake flask, centrifugation and pressurized hot water) using various extractants (organic solvents, hot water and pressurized hot water) were adopted to investigate the in vitro antiglycation activity. Central composite (CCD) design based Response Surface Methodology (RSM) was espoused to optimize the extraction process of polyphenols from the fruit seeds that have recorded poor antiglycation activity. The PTLC analysis was performed to isolate the polyphenols (Flavonoids and phenolic acids) and LC-PDA-MS analysis was done for structure prediction. Pressurized hot water extraction of Artocarpus heterophyllus (87.52%) and Citrus sinensis seeds (74.79%) was found to possess high and low antiglycation activity, respectively. The RSM mediated optimization process adopted for the Citrus sinensis seeds have revealed that 1:15 solvent ratio (hexane to heptane), 6 minutes and 1:20 solid to liquid ratio as the optimal conditions for the extraction of polyphenols with a maximum antiglycation activity (89.79%). The LC-PDA-MS analysis of preparative thin layer chromatography (PTLC) eluates of Artocarpus heterophyllus seed has showed the presence of compounds

  10. Extraction process optimization of polyphenols from Indian Citrus sinensis – as novel antiglycative agents in the management of diabetes mellitus

    Science.gov (United States)

    2014-01-01

    Background Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. It has become an epidemic disease in the 21st century where, India leads the world with largest number of diabetic subjects. Non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (AGEs) that leads to the other complicated secondary disorders. In this context, Mangifera indica (Mango), Syzygium cumini (Jambul), Vitis vinifera (Grapes), Citrus sinensis (Orange), Artocarpus heterophyllus (Jackfruit), Manilkara zapota (Sapodilla) seeds were evaluated for their antiglyation activity. Attempts were made to isolate the polyphenols in the seeds that have recorded the maximum activity. Methods Different extraction methods (shake flask, centrifugation and pressurized hot water) using various extractants (organic solvents, hot water and pressurized hot water) were adopted to investigate the in vitro antiglycation activity. Central composite (CCD) design based Response Surface Methodology (RSM) was espoused to optimize the extraction process of polyphenols from the fruit seeds that have recorded poor antiglycation activity. The PTLC analysis was performed to isolate the polyphenols (Flavonoids and phenolic acids) and LC-PDA-MS analysis was done for structure prediction. Results Pressurized hot water extraction of Artocarpus heterophyllus (87.52%) and Citrus sinensis seeds (74.79%) was found to possess high and low antiglycation activity, respectively. The RSM mediated optimization process adopted for the Citrus sinensis seeds have revealed that 1:15 solvent ratio (hexane to heptane), 6 minutes and 1:20 solid to liquid ratio as the optimal conditions for the extraction of polyphenols with a maximum antiglycation activity (89.79%). The LC-PDA-MS analysis of preparative thin layer chromatography (PTLC) eluates of Artocarpus heterophyllus seed has

  11. An Update on Antitumor Activity of Naturally Occurring Chalcones

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    En-Hui Zhang

    2013-01-01

    Full Text Available Chalcones, which have characteristic 1,3-diaryl-2-propen-1-one skeleton, are mainly produced in roots, rhizomes, heartwood, leaves, and seeds of genera Angelica, Sophora, Glycyrrhiza, Humulus, Scutellaria, Parartocarpus, Ficus, Dorstenia, Morus, Artocarpus, and so forth. They have become of interest in the research and development of natural antitumor agents over the past decades due to their broad range of mechanisms including anti-initiation, induction of apoptosis, antiproliferation, antimetastasis, antiangiogenesis, and so forth. This review summarizes the studies on the antitumor activity of naturally occurring chalcones and their underlying mechanisms in detail during the past decades.

  12. Proporsi penggunaan berbagai jenis daun tanaman untuk pakan ternak kambing pada lokasi dan ketinggian berbeda di wilayah Malang Raya

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    Sri Susanti

    2016-12-01

    Full Text Available This research aims at investigating the proportion of several types of tree foliages leaves as animal feed in different locations and altitudes in Malang Raya. The case study was conducted in five districts of Malang Raya where were assumed as the centre of goat farms. The results showed that there were 30 types of forage used as goat feeds, both leaves of tree foliages and shrubs, or grasses, crop residues and others. Most forages (73% were leaves of tree foliages and shrubs, both legumes, and non-legumes. Tree foliage leaves were more used as goat feeds in the study area with higher altitude, whereas in the area with lower altitude, farmers also utilized grasses and crop residues. The leaves of tree foliages were Paraserianthes falcataria, Gliricidia sepium, Artocarpus heterophyllus, Leucaena leucocephala and Calliandra calothyrsus. Paraserianthes falcataria was most widely used in Klampok-Singosari (88.3%. The proportion of Calliandra calothyrsus ranged from 32 to 98%, while Gliricidia sepium was 58-98%, both were most widely used in Argoyuwono-Ampelgading. Artocarpus heterophyllus was most widely used in Wajak (90.3%, whereas Leucaena leucocephala leaf was used by all respondents (100% in Sumberdem-Wonosari. It is necessary to evaluate the quality of these tree foliage leaves to provide a better animal feed through supplementation technology. Keyword: altitudes, foliages, leave, location

  13. Trichomes that secrete substances of a mixed nature in the vegetative and reproductive organs of some species of Moraceae

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    Bruna Noce Schnetzler

    Full Text Available ABSTRACT This study aimed to obtain new information about the distribution, morphology and content of secretory trichomes present in the vegetative and reproductive organs of four species of Moraceae: Artocarpus heterophyllus, Dorstenia cayapia, Maclura tinctoria and Sorocea bonplandii. Stem, leaf, flower and inflorescence samples were processed for scanning electron and light microscopy. The species have secretory trichomes on the leaf blade, petiole and stem and also on the inflorescence and flowers of D. cayapia and S. bonplandii and on the peduncle of the inflorescence in A. heterophyllus. These trichomes are of the capitate type in all species, but A. heterophyllus also possess peltate secretory trichomes. Both trichome types exhibit a multicellular head and a unicellular peduncle. Peduncle size and head cell number varies according to species. Peltate trichomes located in depressions were found only in species of Artocarpus, and so should be of diagnostic value for the genus. In all species the trichomes secrete polysaccharides, alkaloids and lipids; phenolic compounds occur in A. heterophyllus and M. tinctoria trichomes. These trichomes probably act in the protection against herbivores, pathogens, desiccation and/ or UV radiation. Moreover, the trichomes found in the inflorescence of D. cayapia may exert a function in pollen grain adhesion.

  14. Host Plant and Leaf-Age Preference of Luprops tristis (Coleoptera: Tenebrionidae: Lagriinae: Lupropini: A Home Invading Nuisance Pest in Rubber Plantation Belts

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    Sabu K. Thomas

    2012-01-01

    Full Text Available Massive seasonal invasion by the litter-dwelling beetle Luprops tristis, into residential buildings prior to monsoon rains, and their prolonged state of dormancy render them a very serious nuisance pest in rubber plantations in the Western Ghats in southern India. Feeding preferences of L. tristis towards leaf litter of seven trees co-occurring in rubber plantations, cashew (Anacardium occidentale, mango (Mangifera indica, jackfruit (Artocarpus heterophyllus, wild jack (Artocarpus hirsutus, cocoa (Theobroma cacao, cassia (Cassia fistula, sapota (Manilkara zapota and rubber (Hevea brasiliensis were analyzed with no-choice and multiple-choice leaf disc tests. Results showed that L. tristis is a generalist feeder with a defined pattern of preference, with the leaf litter of rubber being the most preferred followed by those of jackfruit and cocoa. Tender leaves were preferred over mature leaves except for cocoa and sapota. Equal preference towards tender and mature cocoa leaves, presence of patches of cocoa plantations and the scarce distribution of other host plants in rubber plantation belts leads to the proposal that in the absence of tender and mature rubber leaves, cocoa becomes the major host plant of L. tristis.

  15. Prebiotics – A Review

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    Paiboon Thammarutwasik

    2009-10-01

    Full Text Available Nutraceuticals and functional foods have become an important tool for consumers to manage their health and wellness.Pre-, pro-, and synbiotics are a part of this group of products shown to have properties that can modulate gastrointestinalproblems and improve general health and well being. Prebiotics are polysaccharides that can withstand acidic and enzymaticdigestion in the small intestine and can be utilized by probiotics, and gut microflora, in the large intestine for their growth andactivities that benefit the host’s health, e.g., by enhancing the immunity and mineral absorption, preventing colon cancer andother gastrointestinal diseases, and lowering cholesterol. Prebiotics, e.g., inulin, oligofructose, oligolactose, and lactulose, occur naturally in many plants, or may be synthesized from starch or other carbohydrates using appropriate enzymes.A number of plants from southern Thailand, e.g., palm fruit (Borrassus flabellifer L., jackfruit (Artocarpus heterophyllusLam., young coconut (Cocos nucifera Linn., rambutan (Nephelium lappaceum L., jampadah (Artocarpus integer Merr., and okra (Abelmoschus esculentus Moench. contain a considerable amount of polysaccharides that have been shown to have prebiotic properties. Prebiotics may be used as ingredients in functional foods, or may be presented as nutraceuticals in the form of capsules, tablets or powder, sometimes together with probiotic cultures.

  16. UJI TOKSISITAS (Brine Shrimp Lethality Test DAN AKTIVITAS ANTIOKSIDAN DARI DAUN SINTRONG (Crassocephalum Crepidioides DENGAN METODE 2,2-diphenyl-1-picrylhidrazil (DPPH

    Directory of Open Access Journals (Sweden)

    Fiktor Boni Pasilala

    2016-11-01

    Full Text Available The phytochemical test, brine shrimp lethality test and antioxidant activity test on secondary metabolits of terap (Artocarpus odoratissimus B. leaves has been completed. The leaves samples are extracted by masseration method that is concentrated by using rotary evaporator. The total extract are fractioned with n-hexane and ethyl acetate. Based on secondary metabolits phytochemical test of terap (Artocarpus odoratissimus B. leaves showed that total extract and Ethyl Acetat fraction extract contains alkaloids, phenolics, flavonoids, saponins, steroids and triterpenoids. N-hexane fraction extract contain alkaloids, steroids and tritepenoids. In brine shrimp lethality test, the increase larvae death data was recorded and processed using SAS Probit Analysis to determine the Lethal Concentration 50 % (LC50 value. The results of this test showed that the most active extract is metanol extract with LC50 value of 88.0227 ppm. Based on the antioxidant activity by scavenging activity of DPPH used spectrophotometry was obtained that Inhibition Concentration 50% (IC50 of total extract is 369.0833 ppm, extract of n-hexane fraction is 1532.267 ppm and extract of ethyl acetat fraction is 82.89003 ppm.

  17. BEBERAPA TUMBUHAN OBAT ASAL KALIMANTAN TIMUR SEBAGAI SUMBER SAPONIN POTENSIAL

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    Laode Rijai

    2012-06-01

    Full Text Available Saponin is a class of natural compounds that have activity that is strongly associated with utilization in pharmacy. Exploration has been carried out against a number of secondary metabolite content of medicinal plants in East Kalimantan and some of them found to contain saponins. Plants were found to contain saponins and are considered potential Kokang leaf (Lepisanthes amoena, Kesumbakeling leaf (Bixa orellana, L, Belimbing Wuluh leaf (Averrhoa bilimbi L., Sugi Gadjah leaf (Hyptis capitata, Karamunting leaf (Melastoma malabathricum L, Cempedak bark (Artocarpus champeden, Wijaya Kusuma leaf (Epiphyllum oxipetalum, Langsat seeds (Lansium domesticum, ekor kucing leaf (Acalypha hispida, Kelor bark (Moringa oleifera, Jarong leaf (Stachytarpheta mutabilis, Miana leaf (Coleus atropureus, Jengger Ayam leaf (Celosia cristata, and fruit of Libo (Ficus vargelata. Key words : East borneo medicinal plants, saponins   Abstrak Saponin adalah golongan senyawa alami yang memiliki aktivitas yang sangat terkait dengan pemanfaatan dalam bidang farmasi. Telah dilakukan eksplorasi kandungan metabolit sekunder  terhadap sejumlah tumbuhan obat yang ada di Kalimantan Timur dan beberapa diantaranya terbukti mengandung saponin. Tumbuhan-tumbuhan yang terbukti mengandung sponin dan dianggap potensial adalah daun Kokang (Lepisanthes amoena, daun Kesumbakeling (Bixa orellana, L, daun Belimbing Wuluh (Averrhoa bilimbi L., daun Sugi Gadjah (Hyptis capitata, daun Karamunting (Melastoma malabathricum L, kulit batang Cempedak (Artocarpus champeden, daun Wijaya Kusuma (Epiphyllum oxipetalum, biji Langsat (Lansium domesticum, daun ekor kucing (Acalypha hispida, Kulit Batang Kelor (Moringa oleifera, daun Jarong (Stachytarpheta mutabilis, daun Miana (Coleus atropureus, daun Jengger Ayam (Celosia cristata, buah Libo (Ficus vargelata. Kata Kunci: Tumbuhan Obat Kaltim; Saponin

  18. Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system.

    Science.gov (United States)

    Borgognone, Daniela; Cardarelli, Mariateresa; Rea, Elvira; Lucini, Luigi; Colla, Giuseppe

    2014-04-01

    Leaves of artichoke (Cynara cardunculus L. subsp. scolymus (L.) Hegi) and cardoon (Cynara cardunculus L. var. altilis DC) are traditionally used as herbal medicine. Moderate salt stress could enhance antioxidant activity and phytochemicals in leaves. The aim of this study was to evaluate the effect of chloride salts (NaCl, KCl and CaCl2) on biomass production, mineral composition, phenolic and flavonoid contents and antioxidant activity in leaves of artichoke and cardoon grown in a floating system. In both crops, NaCl and KCl treatments reduced biomass production, while similar values were recorded in CaCl2 and control treatments. In both crops, KCl treatment enhanced total phenolic and flavonoid contents, antioxidant activity and target polyphenols in leaves harvested at 48, 82 and 105 days after sowing (DAS), while leaf quality was improved by NaCl and CaCl2 treatments only at 82 and 105 DAS. Irrespective of salinity, leaves of cardoon had higher total phenolic and flavonoid contents, antioxidant activity and target polyphenols than those of artichoke. The results showed that application of KCl can be considered an effective way to produce high-quality leaves of artichoke and cardoon during the whole cropping cycle, although resulting in a 30% reduction in plant biomass. © 2013 Society of Chemical Industry.

  19. First detailed karyo-morphological analysis and molecular cytological study of leafy cardoon and globe artichoke, two multi-use Asteraceae crops

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    Debora Giorgi

    2016-09-01

    Full Text Available Traditionally globe artichoke and leafy cardoon have been cultivated for use as vegetables but these crops are now finding multiple new roles in applications ranging from paper production to cheese preparation and biofuel use, with interest in their functional food potential. So far, their chromosome complements have been poorly investigated and a well-defined karyotype was not available. In this paper, a detailed karyo-morphological analysis and molecular cytogenetic studies were conducted on globe artichoke (Cynara cardunculus Linnaeus, 1753 var. scolymus Fiori, 1904 and leafy cardoon (C. cardunculus Linneaus, 1753 var. altilis De Candolle, 1838. Fluorescent In Situ Hybridization In Suspension (FISHIS was applied to nuclei suspensions as a fast method for screening of labelling probes, before metaphase spread hybridization. Classic Fluorescent In Situ Hybridization (FISH on slide, using repetitive telomeric and ribosomal sequences and Simple Sequence Repeats (SSRs oligonucleotide as probes, identified homologous chromosome relationships and allowed development of molecular karyotypes for both varieties. The close phylogenetic relationship between globe artichoke and cardoon was supported by the very similar karyotypes but clear chromosomal structural variation was detected. In the light of the recent release of the globe artichoke genome sequencing, these results are relevant for future anchoring of the pseudomolecule sequence assemblies to specific chromosomes. In addition, the DNA content of the two crops has been determined by flow cytometry and a fast method for standard FISH on slide and methodological improvements for nuclei isolation are described.

  20. The genome sequence of the outbreeding globe artichoke constructed de novo incorporating a phase-aware low-pass sequencing strategy of F1 progeny

    Science.gov (United States)

    Scaglione, Davide; Reyes-Chin-Wo, Sebastian; Acquadro, Alberto; Froenicke, Lutz; Portis, Ezio; Beitel, Christopher; Tirone, Matteo; Mauro, Rosario; Lo Monaco, Antonino; Mauromicale, Giovanni; Faccioli, Primetta; Cattivelli, Luigi; Rieseberg, Loren; Michelmore, Richard; Lanteri, Sergio

    2016-01-01

    Globe artichoke (Cynara cardunculus var. scolymus) is an out-crossing, perennial, multi-use crop species that is grown worldwide and belongs to the Compositae, one of the most successful Angiosperm families. We describe the first genome sequence of globe artichoke. The assembly, comprising of 13,588 scaffolds covering 725 of the 1,084 Mb genome, was generated using ~133-fold Illumina sequencing data and encodes 26,889 predicted genes. Re-sequencing (30×) of globe artichoke and cultivated cardoon (C. cardunculus var. altilis) parental genotypes and low-coverage (0.5 to 1×) genotyping-by-sequencing of 163 F1 individuals resulted in 73% of the assembled genome being anchored in 2,178 genetic bins ordered along 17 chromosomal pseudomolecules. This was achieved using a novel pipeline, SOILoCo (Scaffold Ordering by Imputation with Low Coverage), to detect heterozygous regions and assign parental haplotypes with low sequencing read depth and of unknown phase. SOILoCo provides a powerful tool for de novo genome analysis of outcrossing species. Our data will enable genome-scale analyses of evolutionary processes among crops, weeds, and wild species within and beyond the Compositae, and will facilitate the identification of economically important genes from related species. PMID:26786968

  1. Construction of a reference molecular linkage map of globe artichoke (Cynara cardunculus var. scolymus).

    Science.gov (United States)

    Portis, E; Mauromicale, G; Mauro, R; Acquadro, A; Scaglione, D; Lanteri, S

    2009-12-01

    The genome organization of globe artichoke (Cynara cardunculus var. scolymus), unlike other species belonging to Asteraceae (=Compositae) family (i.e. sunflower, lettuce and chicory), remains largely unexplored. The species is highly heterozygous and suffers marked inbreeding depression when forced to self-fertilize. Thus a two-way pseudo-testcross represents the optimal strategy for linkage analysis. Here, we report linkage maps based on the progeny of a cross between globe artichoke (C. cardunculus var. scolymus) and cultivated cardoon (C. cardunculus var. altilis). The population was genotyped using a variety of PCR-based marker platforms, resulting in the identification of 708 testcross markers suitable for map construction. The male map consisted of 177 loci arranged in 17 major linkage groups, spanning 1,015.5 cM, while female map was built with 326 loci arranged into 20 major linkage groups, spanning 1,486.8 cM. The presence of 84 loci shared between these maps and those previously developed from a cross within globe artichoke allowed for map alignment and the definition of 17 homologous linkage groups, corresponding to the haploid number of the species. This will provide a favourable property for QTL scanning; furthermore, as 25 mapped markers (8%) correspond to coding regions, it has an additional value as functional map and might represent an important genetic tool for candidate gene studies in globe artichoke.

  2. Genetic mapping and identification of QTL for earliness in the globe artichoke/cultivated cardoon complex.

    Science.gov (United States)

    Portis, Ezio; Scaglione, Davide; Acquadro, Alberto; Mauromicale, Giovanni; Mauro, Rosario; Knapp, Steven J; Lanteri, Sergio

    2012-05-23

    The Asteraceae species Cynara cardunculus (2n = 2x = 34) includes the two fully cross-compatible domesticated taxa globe artichoke (var. scolymus L.) and cultivated cardoon (var. altilis DC). As both are out-pollinators and suffer from marked inbreeding depression, linkage analysis has focussed on the use of a two way pseudo-test cross approach. A set of 172 microsatellite (SSR) loci derived from expressed sequence tag DNA sequence were integrated into the reference C. cardunculus genetic maps, based on segregation among the F1 progeny of a cross between a globe artichoke and a cultivated cardoon. The resulting maps each detected 17 major linkage groups, corresponding to the species' haploid chromosome number. A consensus map based on 66 co-dominant shared loci (64 SSRs and two SNPs) assembled 694 loci, with a mean inter-marker spacing of 2.5 cM. When the maps were used to elucidate the pattern of inheritance of head production earliness, a key commercial trait, seven regions were shown to harbour relevant quantitative trait loci (QTL). Together, these QTL accounted for up to 74% of the overall phenotypic variance. The newly developed consensus as well as the parental genetic maps can accelerate the process of tagging and eventually isolating the genes underlying earliness in both the domesticated C. cardunculus forms. The largest single effect mapped to the same linkage group in each parental maps, and explained about one half of the phenotypic variance, thus representing a good candidate for marker assisted selection.

  3. Caracterización bromatológica de especies y subproductos vegetales en el trópico húmedo de Colombia

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    Dissa Enith Mosquera Perea

    2013-12-01

    Full Text Available Se determinó el potencial nutritivo para especies pecuarias de recursos locales existentes en el municipio de Quibdó, departamento del Chocó (Colombia, teniendo en cuenta la composición bromatológica y digestibilidad in vitro de la materia seca (DIVMS en hojas de árbol del pan (Arthocarpus altilis Z., pacó (Gustavia superba Kunth. y achín (Colocasia esculenta Linn, cáscaras del fruto de chontaduro (Bractris gasipaess Kunth. y de plátano (Musa paradisiaca L.. El análisis bromatológico incluyó materia seca (MS, proteína bruta (PB, ceniza (Cen, fibra detergente ácida (FDA, fibra detergente neutra (FDN, lignina detergente ácida (LDA y energía bruta (EB. Las hojas de achín y de árbol del pan presentaron los contenidos más altos de PB (27.78 y 19.38% y de EB (3911 y 3981 cal/g, respectivamente. Los valores de DIVMS más altos se presentaron en cáscaras de chontaduro (78.9% y hojas de achín (68.6%. En general, los recursos en estudio presentan valor nutritivo similar al de forrajes cultivados, por tanto, son una alternativa posible para la sustitución parcial de materias primas costosas en dietas para animales de granja.

  4. Effects of tropical high tannin non legume and low tannin legume browse mixtures on fermentation parameters and methanogenesis using gas production technique.

    Science.gov (United States)

    Seresinhe, T; Madushika, S A C; Seresinhe, Y; Lal, P K; Orskov, E R

    2012-10-01

    In vitro experiments were conducted to evaluate the suitability of several mixtures of high tanniniferous non legumes with low tanniniferous legumes on in vitro gas production (IVGP), dry matter degradation, Ammonia-N, methane production and microbial population. Eight treatments were examined in a randomized complete block design using four non-legumes and two legumes (Carallia integerrima×Leucaena leucocephala (LL) (Trt 1), C. integerrima×Gliricidia sepium (GS) (Trt 2), Aporosa lindeliyana×LL (Trt 3), A. lindeliyana×GS (Trt 4), Ceiba perntandra×LL (Trt 5), C. perntandra×GS (Trt 6), Artocarpus heterophyllus×LL (Trt 7), A. heterophyllus×GS (Trt 8). The condensed tannin (CT) content of non legumes ranged from 6.2% (Carallia integerrima) to 4.9% (Ceiba perntandra) while the CT of legumes were 1.58% (Leucaena leucocephala) and 0.78% (Gliricidia sepium). Forage mixtures contained more than 14% of crude protein (CP) while the CT content ranged from 2.8% to 4.0% respectively. Differences (pheterophyllus×L. leucocephala (Trt 7) and A. heterophyllus×G. sepium (Trt 8). Highest (p>0.05) NH3-N (ml/200 mg DM) production was observed with the A. heterophyllus×G. sepium (Trt 8) mixture which may be attributed with it's highest CP content. The correlation between IVGP and CT was 0.675 while IVGP and CP was 0.610. In vitro dry matter degradation (IVDMD) was highest in Trt 8 as well. Methane production ranged from 2.57 to 4.79 (ml/200 mg DM) to be synonimous with IVGP. A higher bacteria population (pArtocarpus heterophyllus+G. sepium (Trt 8) and the same trend was observed with the protozoa population as well. The results show that supplementing high tannin non leguminous forages by incremental substitution of legume forage increased gas production parameters, NH3-N, IVDMD and microbial population in the fermentation liquid. Methane production was not significantly affected by the presence of CT or different levels of CP in forage mixtures. Among non legumes, Ceiba

  5. Effects of Tropical High Tannin Non Legume and Low Tannin Legume Browse Mixtures on Fermentation Parameters and Methanogenesis Using Gas Production Technique

    Directory of Open Access Journals (Sweden)

    T. Seresinhe

    2012-10-01

    Full Text Available In vitro experiments were conducted to evaluate the suitability of several mixtures of high tanniniferous non legumes with low tanniniferous legumes on in vitro gas production (IVGP, dry matter degradation, Ammonia-N, methane production and microbial population. Eight treatments were examined in a randomized complete block design using four non-legumes and two legumes (Carallia integerrima×Leucaena leucocephala (LL (Trt 1, C. integerrima×Gliricidia sepium (GS (Trt 2, Aporosa lindeliyana×LL (Trt 3, A. lindeliyana×GS (Trt 4, Ceiba perntandra×LL (Trt 5, C. perntandra×GS (Trt 6, Artocarpus heterophyllus×LL (Trt 7, A. heterophyllus×GS (Trt 8. The condensed tannin (CT content of non legumes ranged from 6.2% (Carallia integerrima to 4.9% (Ceiba perntandra while the CT of legumes were 1.58% (Leucaena leucocephala and 0.78% (Gliricidia sepium. Forage mixtures contained more than 14% of crude protein (CP while the CT content ranged from 2.8% to 4.0% respectively. Differences (p0.05 NH3-N (ml/200 mg DM production was observed with the A. heterophyllus×G. sepium (Trt 8 mixture which may be attributed with it’s highest CP content. The correlation between IVGP and CT was 0.675 while IVGP and CP was 0.610. In vitro dry matter degradation (IVDMD was highest in Trt 8 as well. Methane production ranged from 2.57 to 4.79 (ml/200 mg DM to be synonimous with IVGP. A higher bacteria population (p<0.05 was found in C. perntandra×G. sepium (Trt 6 followed by Artocarpus heterophyllus+G. sepium (Trt 8 and the same trend was observed with the protozoa population as well. The results show that supplementing high tannin non leguminous forages by incremental substitution of legume forage increased gas production parameters, NH3-N, IVDMD and microbial population in the fermentation liquid. Methane production was not significantly affected by the presence of CT or different levels of CP in forage mixtures. Among non legumes, Ceiba perntandra and Artocarpus

  6. Screening of polysaccharides from tamarind, fenugreek and jackfruit seeds as pharmaceutical excipients.

    Science.gov (United States)

    Nayak, Amit Kumar; Pal, Dilipkumar; Santra, Kousik

    2015-08-01

    The paper describes the isolation and screening of plant polysaccharides namely tamarind seed polysaccharide (TSP), fenugreek seed mucilage (FSM) and jackfruit seed starch (JFSS) from tamarind (Tamarindus indica L.) seeds, fenugreek (Trigonella foenum-graecum L.) seeds and jackfruit (Artocarpus heterophyllus L.) seeds, respectively. The yields of isolated dried TSP, FSM and JFSS were 47.00%, 17.36% and 18.86%, respectively. Various physicochemical properties like colour, odour, taste, solubility in water, pH and viscosity of these isolated plant polysaccharides were assessed. Isolated polysaccharide samples were subjected to some phytochemical identification tests. FTIR and (1)H NMR analyses of isolated polysaccharides were performed, which suggest the presence of sugar residues. Isolated TSP, FSM and JFSS can be used as pharmaceutical excipients in various pharmaceutical formulations. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Structural characterization of rubber from jackfruit and euphorbia as a model of natural rubber.

    Science.gov (United States)

    Mekkriengkrai, Dararat; Ute, Koiichi; Swiezewska, Ewa; Chojnacki, Tadeusz; Tanaka, Yasuyuki; Sakdapipanich, Jitladda T

    2004-01-01

    A structural study of low molecular weight rubbers from Jackfruit (Artocarpus heterophyllus) and Painted spurge (Euphorbia heterophylla) was carried out as model compounds of natural rubber from Hevea brasiliensis. The rubber content of latex from Jackfruit was 0.4-0.7%, which is very low compared with that of 30-35% in the latex from Hevea tree. The rubber from Jackfruit latex was low molecular weight with narrow unimodal molecular weight distribution (MWD), whereas that obtained from E. heterophylla showed very broad MWD. The 1H and 13C NMR analyses showed that Jackfruit rubber consists of a dimethylallyl group and two trans-isoprene units connected to a long sequence of cis-isoprene units. The alpha-terminal group of Jackfruit rubber was presumed to be composed of a phosphate group based on the presence of 1H NMR signal at 4.08 ppm corresponding to the terminal =CH-CH2OP group.

  8. Lectins with Anti-HIV Activity: A Review

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    Ouafae Akkouh

    2015-01-01

    Full Text Available Lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-HIV activity. The anti-HIV plant lectins include Artocarpus heterophyllus (jacalin lectin, concanavalin A, Galanthus nivalis (snowdrop agglutinin-related lectins, Musa acuminata (banana lectin, Myrianthus holstii lectin, Narcissus pseudonarcissus lectin, and Urtica diocia agglutinin. The anti-HIV algal lectins comprise Boodlea coacta lectin, Griffithsin, Oscillatoria agardhii agglutinin. The anti-HIV cyanobacterial lectins are cyanovirin-N, scytovirin, Microcystis viridis lectin, and microvirin. Actinohivin is an anti-HIV actinomycete lectin. The anti-HIV worm lectins include Chaetopterus variopedatus polychaete marine worm lectin, Serpula vermicularis sea worm lectin, and C-type lectin Mermaid from nematode (Laxus oneistus. The anti-HIV nonpeptidic lectin mimics comprise pradimicins and benanomicins. Their anti-HIV mechanisms are discussed.

  9. Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM.

    Science.gov (United States)

    Liu, Y; Cecílio, N T; Carvalho, F C; Roque-Barreira, M C; Feizi, T

    2015-12-01

    This article contains data related to the researc.h article entitled "Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM" by Cecílio et al. (2015) [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exerts immunomodulatory and regenerative activities through its Carbohydrate Recognition Domain (CRD) (Souza et al., 2013; Mariano et al., 2014 [2], [3]). The limited availability of the native lectin (n-ArtinM) led us to characterize a recombinant form of the protein, obtained by expression in Saccharomyces cerevisiae (y-ArtinM). We compared the carbohydrate-binding specificities of y-ArtinM and n-ArtinM by analyzing the binding of biotinylated preparations of the two lectin forms using a neoglycolipid (NGL)-based glycan microarray. Data showed that y-ArtinM mirrored the specificity exhibited by n-ArtinM.

  10. From waste to high-value product: Jackfruit peel derived pectin/apatite bionanocomposites for bone healing applications.

    Science.gov (United States)

    Govindaraj, Dharman; Rajan, Mariappan; Hatamleh, Ashraf A; Munusamy, Murugan A

    2018-01-01

    Public requirements encouraged by the current asset framework drive industry to expand its general effectiveness by enhancing existing procedures or finding new uses for waste. Thus, the aim of this study was the isolation, fabrication, and characterization of pectin derived from jackfruit (Artocarpus heterophyllus) peels and the generation of hybrid of pectin (P)/apatite (HA) (P/HA) bionanocomposites. In this process, the natural pectin polymer derived from the peel of jackfruits was used in different concentrations for the fabrication of HA bionanocomposites. Characterization of the isolated pectin and bionanocomposites samples was performed with 1 H NMR and 13 C NMR, FTIR, XRD, SEM-EDX, and HR-TEM. Cytocompatibility, ALP, fibroblast stem cells, anti-inflammatory and cell adhesion testing of the fabricated bionanocomposites was showed good biocompatibility. Our results signify that the fabricated bionanocomposites might be applicable as bone graft materials. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Jackfruit anaphylaxis in a latex allergic patient.

    Science.gov (United States)

    Wongrakpanich, Supakanya; Klaewsongkram, Jettanong; Chantaphakul, Hiroshi; Ruxrungtham, Kiat

    2015-03-01

    Several fruits have been reported to crossreact with latex antigen in latex allergy patients but little is known regarding tropical fruits in particular. Here we report the case of a 34-year old nurse who developed anaphylaxis following the ingestion of dried jackfruit (Artocarpus heterophyllus). The patient had a history of chronic eczema on both hands resulting from a regular wear of latex gloves. She and her family also had a history of atopy (allergic rhinitis and/or atopic dermatitis). The results of skin prick tests were positive for jackfruit, latex glove, kiwi and papaya, but the test was negative for banana. While we are reporting the first case of jackfruit anaphylaxis, further research needs to be conducted to identify the mechanisms underlying it. In particular, in-vitro studies need to be designed to understand if the anaphylaxis we describe is due to a cross reactivity between latex and jackfruit or a coincidence of allergy to these 2 antigens.

  12. Seleksi Pohon untuk Sarang Kuntul Kerbau (Bubulcus ibis di Dusun Wisata Ketingan Kab. Sleman Daerah Istimewa Yogyakarta

    Directory of Open Access Journals (Sweden)

    Eko Kosasih

    2011-07-01

    Full Text Available Habitat selection of Bubulcus ibis in Ketingan Village, Sleman Regency, Yogyakarta was investigated to provide basic information for population management. Presence of Bubulcus ibis nest was surveyed along with identification available tree species. Tree height, canopy size and openness, and distance from human disturbance were also measured for each tree. The Chi Square test showed that bubulcus ibis selected several species as nest trees such as Gnetum gnemon L., Cassia siamea Lamk., Artocarpus heterophyllus Lamk., Calophyllum inophyllum L., Melia azedarach L., Antidesma bunius (L. Spreng., and Dysoxylum gaudichaudianum(Juss. Miq. Tree height, canopy height, canopy diameter and the distance from human disturbance showed a significant difference between used and non-used trees for the nest, however diameter breast high (DBH and canopy openness of trees did not show significant differences. Habitat management of Bubulcus ibis should be improved by controlling its population that needed for the conservation of this bird species.

  13. Use of exotic species in afforestation and facilitation for the establishment of biological invasion

    Directory of Open Access Journals (Sweden)

    Juliano Ricardo Fabricante

    2017-02-01

    Full Text Available This study aimed to inventory the species used in landscaping the Campus of Agricultural Sciences of the Federal University of Paraíba, Areia, PB, Brazil and to rank them according to their origin and their invasive potential. Through walks throughout the study area (active search, we cataloged all the species used in local afforestation and classified them as native or exotic. Exotic plants were also classified as to their invasive potential. Altogether, we identified 76 species belonging to 67 genera and 25 families. Of these, only 26 species were native. The results of this study are worrisome because of the large number of exotic species used for planting at the study site (50 species, including known aggressive species: Artocarpus heterophyllus Lam., Azadirachta indica A. Juss. and Leucaena leucocephala (Lam. de Wit.

  14. Accumulation of primary and secondary metabolites in edible jackfruit seed tissues and scavenging of reactive nitrogen species.

    Science.gov (United States)

    Fernandes, Fátima; Ferreres, Federico; Gil-Izquierdo, Angel; Oliveira, Andreia P; Valentão, Patrícia; Andrade, Paula B

    2017-10-15

    Studies involving jackfruit tree (Artocarpus heterophyllus Lam.) focus on its fruit. Nevertheless a considerable part of jackfruit weight is represented by its seeds. Despite being consumed in several countries, knowledge about the chemical composition of these seeds is scarce. In this work, the accumulation of primary and secondary metabolites in jackfruit seed kernel and seed coating membrane was studied. Sixty-seven compounds were identified, sixty of them being reported for the first time in jackfruit seed. Both tissues had a similar qualitative profile, but significant quantitative differences were found. The capacity of aqueous extracts from jackfruit seed kernel and seed coating membranes to scavenge nitric oxide radical was also evaluated for the first time, the extract prepared from the seed coating membrane being the most potent. This work increases the potential revenue from a food that is still largely wasted. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Anti-inflammatory effect of ethanolic extract of spine, skin and rind of Jack fruit peel - A comparative study.

    Science.gov (United States)

    Meera, M; Ruckmani, A; Saravanan, R; Lakshmipathy Prabhu, R

    2017-10-09

    The present study was conducted to identify the chemical constituents and evaluate the anti-inflammatory activity of crude ethanolic extracts of spine, skin and rind of jack fruit (Artocarpus heterophyllus) peel. Polyphenol and flavonoid contents were assessed using Folin's Ciocalteu reagent and aluminium chloride methods which revealed 316, 355 and 382 mg tannic acid equivalent/g of polyphenol and 96.7, 131.6 and 164.6 mg quercetin equivalent/g of flavonoid in spine, skin and rind, respectively. Anti-inflammatory activity of all three extracts was comparable to diclofenac in vitro and in vivo studies. Skin exhibited maximum anti-inflammatory activity, rind had preferential inhibition on Cyclooxygenase-2 and spine and skin inhibited both Cyclooxygenase-1 and 2 in vitro.

  16. Study of Acid Hydrolysis on Organic Waste: Understanding The Effect of Delignification and Particle Size

    Directory of Open Access Journals (Sweden)

    Anwar Nadiem

    2018-01-01

    Full Text Available Organic wastes from Swiettenia marcophylla L, Artocarpus heterophyllus L, Mangifera indica L, and Annona muricata L were prepared by grinding into 0.1875, 0.3750, 0.7500 mm of particle size and delignified by 2% NaOH at 80°C for 90 minutes. Acid dilution hydrolysis process with H2SO4 1% was performed at 150°C for 120 minutes in a closed reactor. The effect of particle size and delignification on and reducing sugar concentration were investigated. The result showed (1 leaves that can be used as raw material to produce hydrogen should have 38–49% cellulose and hemicellulose. (2 Reducing sugar concentration increased with particle size reduction and delignification. (3 the best result with the highest reducing sugar concentration was achieved by 0.1875 mm particle size with delignification on Annona muricata L.

  17. Cultivated plants in the diversified homegardens of local communities in Ganges Valley, Bangladesh

    DEFF Research Database (Denmark)

    Rahman, Syed Ajijur; Baldauf, Cristina; Mollee, Eefke Maria

    2013-01-01

    Homestead agroforestry, in the form of homegardens, has a long tradition in many developing countries. These systems are an intimate mix of diversified agricultural crops and multipurpose trees planted, maintained by members of the household. This paper aims to explore the species composition...... observation, (3) informal and structured interviews with a purposive sample of 90 households. A total of 53 plant species under 32 families were identified from the study area and it was found that the relative density were highest for Areca catechu (areca palm), Artocarpus heterophyllus (jackfruit....... The comparison of Shannon-Wiener index between agroforestry systems and natural forest showed no statistical difference, reinforcing the role of homegardens in biological conservation in Bangladesh. Therefore, increasing agroforestry practices in homesteads, should be the strategy for enhancing tree cover...

  18. Interaction of a novel Tn (GalNAc alpha 1-->Ser/Thr) glycoprotein with Gal, GalNAc and GlcNAc specific lectins.

    Science.gov (United States)

    Wu, A M; Wu, J H; Shen, F

    1994-01-14

    A naturally occurring Tn glycoprotein (Native ASG-Tn) with GalNAc alpha 1-->Ser/Thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. In a quantitative precipitin assay, this glycoprotein completely precipitated Maclura pomifera (MPA), Vicia villosa B4 (VVL-B4) and Artocarpus integrifolia (Jacalin, AIL). It also reacted well with Helix pomatia (HPL) and Wistaria floribunda (WFL) and precipitated over 75% of the lectin nitrogen added, but poorly with Ricinus communis agglutinin (RCA1), ricin, peanut (Arachis hypogaea, PNA), Abrus precatorius agglutinin (APA) and Triticum vulgaris (WGA). This finding suggests that this novel Tn-glycoprotein may serve as a useful reagent for differentiating Tn and T specific monoclonal antibodies and lectins.

  19. Lectins in fish skin: do they play a role in host-monogenean interactions?

    Science.gov (United States)

    Buchmann, K

    2001-09-01

    Mucus samples from rainbow trout skin with or without infections by Gyrodactylus derjavini were tested for the presence of lectins reacting with mannose, galactose and lactose. The samples inhibited the binding of biotinylated lectins (from Canavalia ensiformis, Artocarpus integrifolia and Erythrina corallodendron, respectively) to microtitre plates with covalently bound carbohydrates (mannopyranoside, galactopyranoside and lactose, respectively). However, the inhibition of C. ensiformis and A. integrifolia lectins was slightly greater when mucus from infected (but recovering) fish was used, suggesting an increase of mannose and galactose binding lectins in fish skin exposed to parasites. As mannose, galactose and lactose are present on the glycocalyx of Gyrodactylus derjavini, it is suggested that lectins could play a dual role in interactions between fish hosts and their monogenean parasites. Thus, recognition between parasite and host and also host responses towards parasite infections could both, at least partly, involve carbohydrate-lectin binding.

  20. Salt tolerances of some mainland tree species select as through nursery screening.

    Science.gov (United States)

    Miah, Md Abdul Quddus

    2013-09-15

    A study of salt tolerance was carried out on germination, survival and height growth performance of important mesophytic species such as Acacia auriculiformis, Acacia hybrid, Artocarpus heterophyllus, Albizia procera, Albizia lebbeck, Acacia nilotica, Achras sapota, Casuarina equisetifolaia, Emblica officinalis, Leucaena leucocephala, Samania saman, Swetenia macrophylla, Terminalia arjuna, Tamarindus indica, Terminalia bellirica and Thespesia populnea in nursery stage using fresh water and salt (NaCl) solutions of 10, 15 and 20 ppm. Effect of salt on germination, survival performance and height growth performance were examined in this condition. Based on the observation, salt tolerance of these species has been determined Acacia auriculiformis, Acacia hybrid, Achras sapota, Casuarina equisetifolia, Leucaena leucocephala and Tamarindus indica has showed the best capacity to perform in different salinity conditions. Acacia nilotica, Emblica officinalis, Thespesia populnea has performed better. Albizia procera, Samania saman and Terminalia bellirica, germination and height performance showed good but when salinity increases survivability were decreases.

  1. Enhancing adsorption capacity of toxic malachite green dye through chemically modified breadnut peel: equilibrium, thermodynamics, kinetics and regeneration studies.

    Science.gov (United States)

    Chieng, Hei Ing; Lim, Linda B L; Priyantha, Namal

    2015-01-01

    Breadnut skin, in both its unmodified (KS) and base-modified (BM-KS) forms, was investigated for its potential use as a low-cost adsorbent for the removal of toxic dye, malachite green (MG). Characterization of the adsorbents was carried out using scanning electron microscope, X-ray fluorescence and Fourier transform infra-red spectroscopy. Batch adsorption experiments, carried out under optimized conditions, for the adsorption of MG were fitted using five isotherm models (Langmuir, Freundlich, Dubinin-Radushkevich, Temkin and Sips) and six error functions to determine the best-fit model. The adsorption capacity was greatly enhanced when breadnut skin was chemically modified with NaOH, leading to an adsorption capacity of 353.0 mg g(-1), that was far superior to most reported adsorbents for the removal of MG. Thermodynamics studies indicated that the adsorption of MG was spontaneous on KS and BM-KS, and the reactions were endothermic and exothermic, respectively. Kinetics studies showed that both followed the pseudo-second order. Regeneration experiments on BM-KS indicated that its adsorption capacity was still maintained at>90% even after five cycles. It can be concluded that NaOH-modified breadfruit skin has great potential to be utilized in real-life application as a low-cost adsorbent for the removal of MG in wastewater treatment.

  2. Rundown

    Directory of Open Access Journals (Sweden)

    Richard Price

    1993-01-01

    Full Text Available [First paragraph] Our 1993 rundown of books that have not, for one reason or another, been reviewed in the NWIG follows the culinary metaphors of its precedents, "Caribbean pepper-pot" (NWIG 58:89-98 and "Callaloo" (NWIG 66:95-99. Cassidy & Le Page's Dictionary ofJamaican English (Cambridge University Press, 1967 offers, s.v. rundown: A kind of sauce made by boiling coconut down till it becomes like custard (but stops short of becoming oil. In it may be cooked salt or pickled fish, banana, or other ingredients. It is served in a bowl in the middle of the table, into which one dips one's bread-kind. See dip-and-come-back. Under "dip-and-come-back," we find thirty-six alternative terms for the dish, including: dip-and-fall-back, dip-and-shake-off, assistant, bread-fruit remedy, dip-dip, dividen-an-flabub, duck-and-shake-back, elbow-grease, frigasi, johnny run-down, kobijong, kuochi waata, malongkontong, mulgrave, pakassa, plaba, plomi, rege, round-the-road, stew-down, swimmerdown, tap-i-a-paas....

  3. Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells

    Directory of Open Access Journals (Sweden)

    Carla Oliveira

    2011-01-01

    Full Text Available Frutalin is the α-D-galactose-binding lectin isolated from breadfruit seeds. Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified from Pichia pastoris. This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis. Furthermore, the interaction between frutalin and the HeLa cells was investigated by confocal microscopy. Despite having different carbohydrate-binding affinities, native and recombinant frutalin showed an identical magnitude of cytotoxicity on HeLa cells growth (IC50~100 μg/mL and equally induced cell apoptosis. The interaction studies showed that both lectins were rapidly internalised and targeted to HeLa cell's nucleus. Altogether, these results indicate that frutalin action is not dependent on its sugar-binding properties. This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity.

  4. PREFERENSI KONSUMEN TERHADAP PANCAKE DARI TEPUNG SUKUN

    Directory of Open Access Journals (Sweden)

    Sitti Rosipah

    2016-11-01

    Full Text Available Preference of food product is influenced by personal characteristics (age, sex,education, income, and nutrient knowledge, food characteristics (taste, shape, and texture andalso surroundings characteristics (job, number of family, mobility, and season. Pancake hassome attributes such as texture, colour, taste, and aroma. These attributes will influenceconsumer likes. The aims of this research are to determine the attributes of consumerpreference appraisal and to know the order of consumer preference toward pancake made ofbreadfruit flour. The method used in this research is conjoint analysis. Attributes and theirlevel is determined by asking for some respondents (questionaire to collect data of consumerpreference of this product. The results (measured by utility value of this research are: texture(rather soft 0,029; colour (golden yellow 0,112; taste (sweet 0,021; and aroma (usual 0,566.The priority of consumer’s decision making toward pancake made of breadfruit are: aroma(49,745%, texture (19,925%, colour (19,628%, and taste (10,702%.

  5. Diversity and Abundance of Cerambycid Beetles in the Four Major Land-use Types Found in Jambi Province, Indonesia

    Directory of Open Access Journals (Sweden)

    Fahri

    2016-04-01

    Full Text Available Longhorn beetles (Coleoptera: Cerambycidae have an important function in the ecosystem, i.e. bioindicators, saproxylic, pollinators, and as food of other organisms. Land cover changes due to land use can disrupt the natural balance of the ecosystem, which can result in a decrease of cerambycid diversity. Cerambycid species diversity was evaluated in four land types, i.e. jungle-rubber, rubber plantations, oil palm plantations, and felled jungle-rubber. Collections of cerambycid beetles were conducted by using artocarpus trap, made by freshly cut Artocarpus heterophyllus branches. Collections of beetles were made on day 4th, 7th, 10th, 13th, and 16th after the traps were set up. In the four land-use types in Jambi province, we collected 72 species including 34 morphospecies of cerambycids, consisting of 42 species from the jungle-rubber, 39 species from rubber plantations, 16 species from oil palm plantations, and 28 species from felled jungle-rubber. Cerambycid diversity was highest in jungle-rubber (H'=3.23, followed by rubber plantation (H'=2.67, felled jungle-rubber (H'=2.38, and oil palm plantations (H'=2.01. Highest similarities of cerambycid communities occurred in the rubber plantation–felled jungle-rubber (51.2, followed by jungle-rubber–rubber plantation (50.0, rubber plantations–oil palm plantations (43.5, oil palm plantation–felled jungle-rubber (42.4, jungle-rubber–oil palm plantations (35.3, and jungle-rubber–felled jungle rubber (34.8. The number of cerambycid species and individuals collected was highest on day 7th.

  6. Distribution of cesium-137 in tree crop products collected from residence islands impacted by the U.S. nuclear test program in the northern Marshall Islands

    International Nuclear Information System (INIS)

    Peters, S.K.G.; Kehl, S.R.; Martinelli, R.E.; Tamblin, M.W.; Hamilton, T.F.

    2013-01-01

    The Marshall Islands Program at the Lawrence Livermore National Laboratory has completed a series of radiological surveys at Bikini, Rongelap, Utroek, and Enewetak Atolls in the Marshall Islands designed to take a representative sample of food supplies with emphasis on determining 137 Cs activity concentrations in common food plants. Coconuts (Cocos nucifera L.) are the most common and abundant food plant, and provided a common sample type to characterize the level and variability of activity concentrations of 137 Cs in plant foods collected from different islands and atolls. Other dominant food types included Pandanus (Pandanus spp.) and breadfruit (Actocarpus spp.). In general, the activity concentration of 137 Cs in food plants was found to decrease significantly between the main residence islands on Bikini, Rongelap, Utrōk, and Enewetak Atolls. The mean activity concentration of 137 Cs measured in drinking coconut meat and juice was 0.72 (95 % CI 0.68-0.77) and 0.34 (95 % CI 0.30-0.38) Bq g -1 , respectively, on Bikini Island; 0.019 (95 % CI 0.017-0.021) and 0.027 (95 % CI 0.023-0.031) Bq g -1 , respectively, on Rongelap Island; 0.010 (95 % CI 0.007-0.013) and 0.007 (95 % CI 0.004-0.009) Bq g -1 , respectively, on Utroek Island; and 0.002 (95 % CI 0.0013-0.0024) and 0.002 (95 % CI 0.001-0.0025) Bq g -1 , respectively, on Enewetak Island. High levels of variability are reported across all islands. These results will be used to improve the accuracy and reliability of predictive dose assessments, help characterize levels of uncertainty and variability in activity concentrations of fallout radionuclides in plant foods, and allow atoll communities to make informed decisions about resettlement and possible options for cleanup and rehabilitation of islands and atolls. (author)

  7. Website application for calculating cesium-137 ingestion doses from consumption of locally grown foods in the Marshall Islands

    International Nuclear Information System (INIS)

    Kehl, S.R.; Hamilton, T.F.; Simpson, A.E.; Freitas, G.D.

    2013-01-01

    Fallout deposition from the US nuclear weapons test program at Bikini and Enewetak Atolls (1946-1958) resulted in widespread nuclear fallout contamination of the northern Marshall Islands. About 85-90 % of the nuclear test-related dose delivered to resident populations is derived from ingestion of cesium-137 ( 137 Cs) contained in locally grown tree-crop food products. The Lawrence Livermore National Laboratory has developed a series of interactive internet applications to provide the public with an open access platform to learn more about radiological conditions in the Marshall Islands. The ingestion dose calculator application described here is one such feature whereby users can calculate hypothetical ingestion doses from 137 Cs based on interactive user input matched to environmental data on the activity concentration of 137 Cs contained in food plants such as coconut, breadfruit, Pandanus, and arrowroot. Users are asked to enter a date, an island and atoll location, a plant food type, and a daily intake amount (highlighted by the number of portions eaten per day in estimated gram equivalents). The application computes the user daily dose and the user equivalent annualized dose, and then compares the results with default settings based on dietary models developed for the Marshall Islands from independent dietary surveys. The default diets are based on a local plus imported food diet (or IA diet model) and an imported foods unavailable diet (or IUA diet model). Environmental data are decay corrected to the date entered by the user using an effective half-life of 137 Cs of 8.5 years (http://marshallislands.llnl.gov). (author)

  8. In-vitro Wound Healing Effect of 15-Hydroxyprostaglandin Dehydrogenase Inhibitor from Plant.

    Science.gov (United States)

    Karna, Sandeep

    2017-01-01

    Prostaglandins (PGs) have short existence in vivo because they are rapidly metabolized by NAD + -dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to 15-ketoprostaglandins. Inhibition of 15-PGDH causes elevated level of PGE 2 in cellular system. It will be valuable for the therapeutic management of diseases requiring elevated PGE 2 levels, like wound healing. Ninety-eight plant samples were screened for the discovery of potent 15-PGDH inhibitor. Among them, top five plant extracts as potent 15-PGDH inhibitor were chosen to determine PGE 2 release from HaCaT (Keratinocyte cell line) cell line. Finally, top 15-PGDH inhibitor was selected to evaluate in vitro wound healing effect on HaCaT scratch model. The inhibitory activity for 15-PGDH inhibitors was evaluated using fluorescence spectrophotometer by measuring the formation of NADH at 468 nm following excitation at 340 nm. Cell viability assay and PGE 2 release was evaluated in HaCaT cell line after treatment of 15-PGDH inhibitors. Scratches were made using sterile 200 μL on HaCaT cell and wound-healing effect was evaluated after treatment of 15-PGDH inhibitor. 15-PGDH inhibitors elevated PGE 2 levels in concentration-dependent manner. Ethanol extract of Artocarpus heterophyllus (EEAH), the most potent 15-PGDH inhibitor (IC 50 = 0.62 µg/mL) with least cytotoxicity (IC 50 = 670 µg/ml), elevated both intracellular and extracellular PGE 2 levels. EEAH facilitated in-vitro wound healing in a HaCaT (Keratinocyte cell line) scratch model. EEAH might apply to treat dermal wounds by elevating PGE 2 levels via COX-1 induction and 15-PGDH inhibition. Biological inactivation of 15-PGDH causes elevated level of PGE 2 which will be useful for the management of disease that requires elevated level of PGE 2 . Abbreviations used: 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, COX: Cyclooxygenase, DTT: Dithiothreitol, DMEM: Dulbecco's modified Eagle's media, EEAH: Ethanol extract of Artocarpus heterophyllus, MRP4

  9. Kajian Beberapa Tumbuhan Obat Yang Digunakan Dalam Pengobatan Malaria Secara Tradisional

    Directory of Open Access Journals (Sweden)

    Ira Indriaty Paskalita Bule Sopi

    2017-02-01

    Full Text Available AbstractMalaria is one of community health problems that can cause death especially to high risk group. Malaria treatment using some antimalarial drugs have been resistance so that there is using medicinal plants into traditional antimalarial treatment that have been tested scientific. There are lots of people that use traditional treatment for healing the diseases. This case shows there’s still strong of community tradition about looking for treatment. One of the diseases whose treatment using traditional and modern medicine is malaria. Malaria is one of acute or chronic often be caused by plasmodium parasites. This review aimed is to describe medicinal plants that used on traditional antimalarial treatment. Review of the literature with search and date collection from various references about medicinal plants which used in traditional antimalarial treatment. Method has been done by reviewing literature with search and the data has been collection then described to be an information that shows about kind of medicinal plants and result testing about them. There are some plants that is those are lime tree (Harmsiopanax aculeatus Harms, red fruit (Pandanus conoideus Lam., bark of jack fruit (Artocarpus champedem, fruit betel (Piper betle (L. R. Br., bark of mundu (Garcinia dulcis Kurz, benalu of mango (Dendrophthoe pentandra, mangosteen (Garcinia mangostana Linn., fruit of Morinda citrifolia L, and sunflower (Helianthus annuus L.. From the result that has been accepted shows active compound content that contained in some kind of medicinal plants which have been tested in traditional antimalarial treatment. Keywords: Plant, medicinal, traditional, malariaAbstrakMalaria merupakan salah satu masalah kesehatan masyarakat yang dapat menyebabkan kematian terutama pada kelompok berisiko tinggi. Pengobatan malaria dengan penggunaan beberapa obat anti malaria sudah mengalami resistensi sehingga perlu adanya pemanfaatan tumbuhan obat dalam pengobatan

  10. Differential binding properties of Gal/GalNAc specific lectins available for characterization of glycoreceptors.

    Science.gov (United States)

    Wu, A M; Song, S C; Sugii, S; Herp, A

    1997-01-01

    Differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. Based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty Gal and/or GalNAc specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of GalNAc alpha 1-->Ser (Thr) of the peptide chain. A scheme of codes for lectin determinants is illustrated as follows: (1) F (GalNAc alpha 1-->3GalNAc), Forssman specific disaccharide--Dolichos biflorus (DBL), Helix pomatia (HPL) and Wistaria floribunda (WFL) lectins. (2) A (GalNAc alpha 1-->3 Gal), blood group A specific disaccharide--Codium fragile subspecies tomentosoides (CFT), Soy bean (SBL), Vicia villosa-A4 (VVL-A4), and Wistaria floribunda (WFL) lectins. (3) Tn (GalNAc alpha 1-->Ser (Thr) of the protein core)--Vicia villosa B4 (VVL-B4), Salvia sclarea (SSL), Maclura pomifera (MPL), Bauhinia purpurea alba (BPL) and Artocarpus integrifolia (Jacalin, AIL). (4) T (Gal beta 1-->3GalNAc), the mucin type sugar sequences on the human erythrocyte membrane(T alpha), T antigen or the disaccharides at the terminal nonreducing end of gangliosides (T beta)--Peanut (PNA), Bauhinia purpurea alba (BPL), Maclura pomifera (MPL), Sophora japonica (SJL), Artocarpus lakoocha (Artocarpin) lectins and Abrus precatorius agglutinin (APA).(5) I and II (Gal beta 1-->3(4)GlcNAc)--the disaccharide residue at the nonreducing end of the carbohydrate chains derived from either N- or O-glycosidic linkage--Ricinus communis agglutinin (RCA1), Datura stramonium (TAL, Thorn apple), Erythrina cristagalli (ECL, Coral tree), and Geodia cydonium (GCL). (6) B (Gal alpha 1-->3Gal), human blood group B specific disaccharide--Griffonia(Banderiaea) simplicifolia B4 (GSI-B4). (7) E (Gal alpha 1-->4Gal), receptors for pathogenic E

  11. Atividades biológicas das lectinas PHA, WGA, jacalina e artocarpina Biological activities of PHA, WGA, jacalin and artocarpin lectins

    Directory of Open Access Journals (Sweden)

    Ana Maria Sell

    2000-05-01

    Full Text Available As lectinas são (glicoproteínas que se ligam a açúcares. O interesse destas moléculas na investigação científica se deve às diversas atividades biológicas a elas atribuídas. Além da identificação de grupos sangüíneos, da caracterização de microorganismos e da estimulação mitogênica de células imunes, as lectinas estão envolvidas nos processos de reconhecimento e das interações celulares. No presente trabalho, revisamos algumas atividades biológicas das lectinas PHA (fitohemaglutinina de Phaseolus vulgaris, WGA (aglutinina de germe de trigo, jacalina e artocarpina (lectinas de Artocarpus integrifolia. PHA, jacalina e artocarpina são mitogênicas para os linfócitos, estimulam a produção de citocinas endógenas, a atração e a ativação de leucócitos. WGA é uma lectina não-mitogênica, no entanto, modula a resposta de defesa estimulando a liberação de superóxido pelos neutrófilos. O entendimento das atividades biológicas permitirá o uso destas moléculas como uma ferramenta útil no diagnóstico e tratamento de muitas doenças.Lectins are ubiquitous (glycoproteins, which exhibit a specific and reversibly carbohydrate binding activity. Because of their structural complexity and variability, they can participate in many physiological functions. Besides the blood groups identification, microorganism characterization and mitogenic immune cell proliferation, they are involved in the cell recognition and cell-cell interaction. In the present work we revised some biological activities of the PHA (Phaseolus vulgaris phytohemagglutinin, WGA (wheat germ agglutinin, jacalin and artocarpin (Artocarpus integrifolia lectins. PHA, jacalin and artocarpin may stimulate lymphocyte proliferation, attract and activate mononuclear cells and stimulate endogenous citokines production. WGA is a nonmitogenic lectin, however it may stimulate neutrophil superoxide production. The understanding of the lectin biological activities may

  12. Formulation optimization and evaluation of jackfruit seed starch-alginate mucoadhesive beads of metformin HCl.

    Science.gov (United States)

    Nayak, Amit Kumar; Pal, Dilipkumar

    2013-08-01

    The present study deals with the formulation optimization of jackfruit (Artocarpus heterophyllus Lam., family: Moraceae) seed starch (JFSS)-alginate mucoadhesive beads containing metformin HCl through ionotropic gelation using 3(2) factorial design. The effect of sodium alginate to JFSS ratio and CaCl2 concentration on the drug encapsulation efficiency (DEE, %), and cumulative drug release at 10h (R10h, %) was optimized. The optimized beads containing metformin HCl showed DEE of 97.48±3.92%, R10h of 65.70±2.22%, and mean diameter of 1.16±0.11mm. The in vitro drug release from these beads was followed controlled-release (zero-order) pattern with super case-II transport mechanism. The beads were also characterized by SEM and FTIR. The swelling and degradation of these beads were influenced by pH of the test medium. The optimized beads also exhibited good mucoadhesivity and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Cytotoxic effect of artocarpin on T47D cells.

    Science.gov (United States)

    Arung, Enos Tangke; Wicaksono, Britanto Dani; Handoko, Yohana Ayupriyanti; Kusuma, Irawan Wijaya; Shimizu, Kuniyoshi; Yulia, Dina; Sandra, Ferry

    2010-10-01

    In our screening projects for anticancer agents from natural resources, artocarpin [6-(3-methyl-1-butenyl)-5,2',4'-trihydroxy-3-isoprenyl-7-methoxyflavone] isolated from wood of jack fruit (Artocarpus heterophyllus) showed potent cytotoxic activity on human T47D breast cancer cells. The mode of action of artocarpin was evaluated by its effect on cell viability, nuclear morphology, cell cycle progression, expression of protein markers for apoptosis, and mitochondrial membrane potential (Delta psi m). These results showed that artocarpin caused a reduction of cell viability in a concentration-dependent manner and an alteration of cell and nuclear morphology. Moreover, the percentage of the sub-G1 phase formation was elevated dose-dependently. Artocarpin induced activation of caspase 8 and 10 as indicated by stronger signal intensity of cleaved-caspase 8 and weaker signal intensity of caspase 10 markers detected after artocarpin treatment. In addition, we also noticed the activation of caspase 3 by artocarpin. There were negligible changes in mitochondrial membrane potential (Delta psi m) due to artocarpin treatment. All together, these data indicated that artocarpin induced apoptosis in T47D cells possibly via an extrinsic pathway.

  14. Blends of jackfruit seed starch-pectin in the development of mucoadhesive beads containing metformin HCl.

    Science.gov (United States)

    Nayak, Amit Kumar; Pal, Dilipkumar

    2013-11-01

    In this work, calcium pectinate-jackfruit (Artocarpus heterophyllus Lam.) seed starch (JFSS) mucoadhesive beads containing metformin HCl were developed through ionotropic-gelation. Effects of pectin and JFSS amounts on drug encapsulation efficiency (DEE), and cumulative drug release after 10 h (R10 h) were optimized using 3(2) factorial design. The optimized calcium pectinate-JFSS beads containing metformin HCl showed DEE of 94.11 ± 3.92%, R10 h of 48.88 ± 2.02%, and mean diameter of 2.06 ± 0.20 mm. The in vitro drug release from these beads was followed controlled-release (zero-order) pattern with super case-II transport mechanism. The beads were also characterized by SEM and FTIR. The pH of test mediums was found critical for swelling and mucoadhesion of these beads. The optimized calcium pectinate-JFSS beads also exhibited good mucoadhesivity and significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration. Copyright © 2013 Elsevier B.V. All rights reserved.

  15. Fungus-insect gall of Phlebopus portentosus.

    Science.gov (United States)

    Zhang, Chun-Xia; He, Ming-Xia; Cao, Yang; Liu, Jing; Gao, Feng; Wang, Wen-Bing; Ji, Kai-Ping; Shao, Shi-Cheng; Wang, Yun

    2015-01-01

    Phlebopus portentosus is a popular edible wild mushroom found in the tropical Yunnan, China, and northern Thailand. In its natural habitats, a gall often has been found on some plant roots, around which fungal fruiting bodies are produced. The galls are different from common insect galls in that their cavity walls are not made from plant tissue but rather from the hyphae of P. portentosus. Therefore we have termed this phenomenon "fungus-insect gall". Thus far six root mealy bug species in the family Pseudococcidae that form fungus-insect galls with P. portentosus have been identified: Formicococcus polysperes, Geococcus satellitum, Planococcus minor, Pseudococcus cryptus, Paraputo banzigeri and Rastrococcus invadens. Fungus-insect galls were found on the roots of more than 21 plant species, including Delonix regia, Citrus maxima, Coffea arabica and Artocarpus heterophyllus. Greenhouse inoculation trials showed that fungus-insect galls were found on the roots of A. heterophyllus 1 mo after inoculation. The galls were subglobose to globose, fulvous when young and became dark brown at maturation. Each gall harbored one or more mealy bugs and had a chimney-like vent for ventilation and access to the gall. The cavity wall had three layers. Various shaped mealy bug wax deposits were found inside the wall. Fungal hyphae invaded the epidermis of plant roots and sometimes even the cortical cells during the late stage of gall development. The identity of the fungus inside the cavity was confirmed by molecular methods. © 2015 by The Mycological Society of America.

  16. Carbonised jackfruit peel as an adsorbent for the removal of Cd(II) from aqueous solution.

    Science.gov (United States)

    Inbaraj, B Stephen; Sulochana, N

    2004-08-01

    The fruit of the jack (Artocarpus heterophyllus) is one of the popular fruits in India, where the total area under this fruit is about 13,460 ha. A significant amount of peel (approximately 2,714-11,800 kg per tree per year) is discarded as agricultural waste, as apart from its use as a table fruit, it is popular in many culinary preparations. Treatment of jackfruit peel with sulphuric acid produced a carbonaceous product which was used to study its efficiency as an adsorbent for the removal of Cd(II) from aqueous solution. Batch experiments were performed as a function of process parameters; agitation time, initial metal concentration, adsorbent concentration and pH. Kinetic analyses made with Lagergren pseudo-first-order, Ritchie second-order and modified Ritchie second-order models showed better fits with modified Ritchie second-order model. The Langmuir-Freundlich (Sips equation) model best defined the experimental equilibrium data among the three isotherm models (Freundlich, Langmuir and Langmuir-Freundlich) tested. Taking a particular metal concentration, the optimum dose and pH required for the maximum metal removal was established. A complete recovery of the adsorbed metal ions from the spent adsorbent was achieved by using 0.01 M HCl.

  17. Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM

    Directory of Open Access Journals (Sweden)

    Y. Liu

    2015-12-01

    Full Text Available This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al. (2015 [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exerts immunomodulatory and regenerative activities through its Carbohydrate Recognition Domain (CRD (Souza et al., 2013; Mariano et al., 2014 [2,3]. The limited availability of the native lectin (n-ArtinM led us to characterize a recombinant form of the protein, obtained by expression in Saccharomyces cerevisiae (y-ArtinM. We compared the carbohydrate-binding specificities of y-ArtinM and n-ArtinM by analyzing the binding of biotinylated preparations of the two lectin forms using a neoglycolipid (NGL-based glycan microarray. Data showed that y-ArtinM mirrored the specificity exhibited by n-ArtinM.

  18. The immunomodulatory effect of plant lectins: a review with emphasis on ArtinM properties.

    Science.gov (United States)

    Souza, Maria A; Carvalho, Fernanda C; Ruas, Luciana P; Ricci-Azevedo, Rafael; Roque-Barreira, Maria Cristina

    2013-10-01

    Advances in the glycobiology and immunology fields have provided many insights into the role of carbohydrate-protein interactions in the immune system. We aim to present a comprehensive review of the effects that some plant lectins exert as immunomodulatory agents, showing that they are able to positively modify the immune response to certain pathological conditions, such as cancer and infections. The present review comprises four main themes: (1) an overview of plant lectins that exert immunomodulatory effects and the mechanisms accounting for these activities; (2) general characteristics of the immunomodulatory lectin ArtinM from the seeds of Artocarpus heterophyllus; (3) activation of innate immunity cells by ArtinM and consequent induction of Th1 immunity; (4) resistance conferred by ArtinM administration in infections with intracellular pathogens, such as Leishmania (Leishmania) major, Leishmania (Leishmania) amazonensis, and Paracoccidioides brasiliensis. We believe that this review will be a valuable resource for more studies in this relatively neglected area of research, which has the potential to reveal carbohydrate targets for novel prophylactic and therapeutic strategies.

  19. 2-Arylbenzo[b]furan derivatives as potent human lipoxygenase inhibitors.

    Science.gov (United States)

    Lang, Li; Dong, Ningning; Wu, Deyan; Yao, Xue; Lu, Weiqiang; Zhang, Chen; Ouyang, Ping; Zhu, Jin; Tang, Yun; Wang, Wei; Li, Jian; Huang, Jin

    2016-01-01

    Human lipoxygenases (LOXs) have been emerging as effective therapeutic targets for inflammatory diseases. In this study, we found that four natural 2-arylbenzo[b]furan derivatives isolated from Artocarpus heterophyllus exhibited potent inhibitory activities against human LOXs, including moracin C (1), artoindonesianin B-1 (2), moracin D (3), moracin M (4). In our in vitro experiments, compound 1 was identified as the most potent LOX inhibitor and the moderate subtype selective inhibitor of 12-LOX. Compounds 1 and 2 act as competitive inhibitors of LOXs. Moreover, 1 significantly inhibits LTB4 production and chemotactic capacity of neutrophils, and is capable of protecting vascular barrier from plasma leakage in vivo. In addition, the preliminary structure-activity relationship analysis was performed based on the above four naturally occurring (1-4) and six additional synthetic 2-arylbenzo[b]furan derivatives. Taken together, these 2-arylbenzo[b]furan derivatives, as LOXs inhibitors, could represent valuable leads for the future development of therapeutic agents for inflammatory diseases.

  20. Leaf Litter Decomposition and Nutrient Dynamics Associated with Common Horticultural Cropland Agroforest Tree Species of Bangladesh

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    Md. Hasanuzzaman

    2014-01-01

    Full Text Available Mangifera indica, Zizyphus jujuba, Litchi chinensis, and Artocarpus heterophyllus are the most common cropland agroforest horticultural tree species of Bangladesh. This study focused on leaf litter decomposition and nutrient (N, P, and K dynamics during the decomposition process. This experiment was conducted for 180 days by using litter bag technique during dry and wet seasons. Mass loss was the highest (49% and 57% for A. heterophyllus and the lowest (25% was found for L. chinensis. The highest initial rates (0.75% and 2.35%/day of decomposition were observed for Z. jujuba and the lowest (0.50% and 0.79%/day for L. chinensis. The highest decay constant was observed for A. heterophyllus (2.14 and 2.34 and the lowest (0.88 and 0.94 for L. chinensis. Leaf litter of all the studied species showed a similar pattern (K > N > P of nutrient release during the decomposition process. Zizyphus jujuba showed comparatively higher return of N, P, and K than others. However, a significant (P<0.05 higher amount of mass loss, rate of decomposition, decay constant, and amount of nutrient return from leaf litter were observed during the wet season.

  1. ArtinM offers new perspectives in the development of antifungal therapy

    Science.gov (United States)

    Ruas, Luciana P.; Carvalho, Fernanda C.; Roque-Barreira, Maria-Cristina

    2012-01-01

    The thermally dimorphic fungus Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis (PCM), the most frequent systemic mycosis that affects the rural populations in Latin America. Despite significant developments in antifungal chemotherapy, its efficacy remains limited since drug therapy is prolonged and associated with toxic side effects and relapses. In response to these challenges, it is now recognized that several aspects of antifungal immunity can be modulated to better deal with fungal infections. A common idea for halting fungal infections has been the need to activate a cell-based, pro-inflammatory Th1 immune response to improve the fungal elimination. ArtinM, a D-mannose binding lectin from Artocarpus heterophyllus, has the property of modulating immunity against several intracellular pathogens. Here, we review the immunomodulatory activity of ArtinM during experimental PCM in mice. Both prophylactic and therapeutic protocols of ArtinM administration promotes a Th1 immune response balanced by IL-10, which outstandingly reduces the fungal load in organs of the treated mice while maintaining a controlled inflammation at the site of infection. A carbohydrate recognition-based interaction of ArtinM with Toll-like receptor 2 (TLR2) accounts for initiating the immunomodulatory effect of the lectin. The precise identification of the TLR2 N-glycan(s) targeted by ArtinM may support novel basis for the development of antifungal therapy. PMID:22715337

  2. Identification of a flavonoid C-glycoside as potent antioxidant.

    Science.gov (United States)

    Wen, Lingrong; Zhao, Yupeng; Jiang, Yueming; Yu, Limei; Zeng, Xiaofang; Yang, Jiali; Tian, Miaomiao; Liu, Huiling; Yang, Bao

    2017-09-01

    Flavonoids have been documented to have good antioxidant activities in vitro. However, reports on the cellular antioxidant activities of flavonoid C-glycosides are very limited. In this work, an apigenin C-glycoside was purified from Artocarpus heterophyllus by column chromatography and was identified to be 2″-O-β-D-xylosylvitexin by nuclear magnetic resonance spectroscopy. The cellular antioxidant activity and anticancer activity of 2″-O-β-D-xylosylvitexin were evaluated for the first time. The quantitative structure-activity relationship was analysed by molecular modeling. Apigenin presented an unexpected cellular antioxidation behaviour. It had an antioxidant activity at low concentration and a prooxidant activity at high concentration, whereas 2″-O-β-D-xylosylvitexin showed a dose-dependent cellular antioxidant activity. It indicated that C-glycosidation improved the cellular antioxidation performance of apigenin and eliminated the prooxidant effect. The ortho-dihydroxyl at C-3'/C-4' and C-3 hydroxyl in the flavonoid skeleton play important roles in the antioxidation behaviour. The cell proliferation assay revealed a low cytotoxicity of 2″-O-β-D-xylosylvitexin. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Utilização de espécies exóticas na arborização e a facilitação para o estabelecimento de casos de invasão biológica

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    Juliano Ricardo Fabricante

    2017-02-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2017v30n1p55 O presente estudo teve como objetivo inventariar as espécies utilizadas na arborização do campus de Ciências Agrárias (CCA da Universidade Federal da Paraíba (UFPB, Areia, PB e classificá-las de acordo com sua origem e seu potencial invasor. Por meio de caminhadas por toda a área de estudo (busca ativa foram inventariadas todas as espécies utilizadas na arborização local e classificadas em nativas e exóticas. As exóticas ainda foram classificadas quanto ao seu potencial invasor. Ao todo foram identificadas 76 espécies pertencentes a 67 gêneros e 25 famílias. Destas, apenas 26 espécies eram nativas. Os resultados do presente trabalho são preocupantes devido ao elevado número de espécies exóticas utilizadas na arborização do local de estudo (50 spp. e pela presença de espécies reconhecidamente agressivas, a exemplo de Artocarpus heterophyllus Lam., Azadirachta indica A. Juss. e Leucaena leucocephala (Lam. de Wit.

  4. ANALISA KEKUATAN TAHANAN LATERAL SAMBUNGAN KAYU-BETON

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    Efa Suriani

    2015-08-01

    Full Text Available A prediction of shear resistance using lag screw is required to obtain a reliable design timber connection against lateral forces and to know how failure mode from that connection.In this study, shear resistance was evaluated by taking shear test of the joint to the lateral load by using monotonic loading and analysis model adopted from EYM (European Yield Model. The types of timber used were Toona sureni (specific gravity 0.45, Swietenia mahogany (specific gravity 0.51 and Artocarpus heterophyllus (specific gravity 0.54 and concrete with actual compressive strength of 15.93 MPa. Lag-screw in 8 mm diameter (length 130 mm, washer in 22.8 mm diameter and fischer S14 were also used. The percentages results of shear resistance (5% offset method between the predictions and experiment were 36,40% until 48,15%. These results showed that shear resistance to predicted equation of EYM was close enough to shear resistance based on the test results

  5. Post-dispersal seed removal by ground-feeding rodents in tropical peatlands, Central Kalimantan, Indonesia

    Science.gov (United States)

    Blackham, Grace V.; Corlett, Richard T.

    2015-01-01

    Forested tropical peatlands in Southeast Asia are being rapidly converted to agriculture or degraded into non-forest vegetation. Although large areas have been abandoned, there is little evidence for subsequent forest recovery. As part of a study of forest degradation and recovery, we used seed removal experiments and rodent surveys to investigate the potential role of post-dispersal seed predation in limiting the regeneration of woody plants. Two 14-day seed removal trials were done in deforested and forested peatland habitat in Central Kalimantan, Indonesia. Seeds of Nephelium lappaceum, Syzygium muelleri, Artocarpus heterophyllus (all animal-dispersed) and Combretocarpus rotundatus (wind-dispersed) were tested. Significantly more seeds (82.8%) were removed in forest than non-forest (38.1%) and Combretocarpus had the lowest removal in both habitats. Most handled seeds were eaten in situ and little caching was observed. Six species of rodents were captured in forest and five in non-forest. The most trapped taxa were three Maxomys spp. in forest (85.5% of individuals) and Rattus tiomanicus in non-forest (74.8%). Camera traps confirmed that rodents were responsible for seed removal. Seed predation in deforested areas, which have a much lower seed rain than forest, may contribute to the low density and diversity of regenerating forest. PMID:26369444

  6. Rumen Fermentation, Blood Metabolites, and Performance of Sheep Fed Tropical Browse Plants

    Directory of Open Access Journals (Sweden)

    D. A. Astuti

    2011-12-01

    Full Text Available The in vitro study was designed to evaluate total gas production, dry matter degradability (DMD, and VFA profile; while in vivo study was designed to evaluate nutrient intakes, blood metabolites, and performance of sheep fed native grass mixed with Calliandra calothyrrus (CC, Leucaena leucochepala (LL, Moringa oleifera (MO, Gliricidea sepium (GS, and Artocarpus heterophyllus (AH. The best three from the in vitro results were used to formulate diets in in vivo study. Sixteen male growing sheep (average BW 20 kg were fed 100% native grass (NG as control; 70% NG + 30% GS; 70% NG + 30% MO; and 70% NG + 30% AH. Nutrient consumptions, DMD, blood metabolites, and sheep performances were analyzed by using Completely Randomized Design. The in vitro results showed that the total gas production and DMD of CC and LL were the lowest (P<0.05 while the highest was found in GS, MO, and AH treatments (P<0.05. Meanwhile, the in vivo results showed that nutrient intakes (DM, CP, and CF of GS and AH rations were the highest. The ADG, concentration of albumin, and globulin in all treatments were similar, while total serum protein, triglycerides, and glucose concentration in MO and AH rations were higher than others. Serum cholesterol concentration in MO ration was the lowest, meanwhile the concentration of IgG was the highest (P<0.05. Supplementation of 30% MO was the best choice for optimum rumen fermentation and maintaining health status of local sheep.

  7. Analisis Vegetasi di Hutan Mbeji Daerah Wonosalam Jombang

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    Anita Munawwaroh

    2016-03-01

    Full Text Available Analisis vegetasi terhadap hutan perlu dilakukan untuk mengetahui keanekaragaman hayati yang terdapat di hutan tersebut sehingga mempermudah didalam melakukan pemeliharaan dan pemberdayaan hutan.Tujuan dari penelitian ini adalah untuk mengetahui keanekaragaman hayati dan struktur vegetasi (frekuensi, kerapatan, dominansi, dan Indeks Nilai Penting di hutan mbeji, Wonossalam, Jombang. Pada penelitian ini digunakan metode point centered quarter. Pennelitian dilakukan pada bulan Mei 2015. Hasil penelitian menujukkan padaHutan Mbeji terdapat 11 jenis pohon yang teridentifikasi dan 15 spesies tumbuhan, dimana ada 10 spesies yang telah teridentifikasiNilai penting  tumbuh-tumbuhan di Hutan Mbeji di daerah Wonosalam Jombang  pada tingkat pohon paling tinggi adalah pohon randu (Ceiba petandra  sebesar 57,42 % sedangkan nilai INP paling rendah dari jenis pohon andong (Rhadamnia cinerea yaitu sebesar 6,06 %. Pada sapling nilai penting yang paling tinggi adalah talas (Colocasia esculenta yaitu sebesar 49,23 %. Sedangkan, nilai INP paling rendah dari jenis nanas (Ananas comosus, nangka (Artocarpus heterophyllus, tapak liman (Elephantopus scaber, semak A, semak B, semak C, semak E, dan semak F yaitu sebesar 6,35 %. Tingginya nilai INP menunjukkan bahwa jenis-jenis tersebut dapat menyesuaikan diri dengan lingkungan sekitarnya yang lebih baik dibanding jenis lainnya.

  8. Current trends of tropical fruit waste utilization.

    Science.gov (United States)

    Cheok, Choon Yoong; Mohd Adzahan, Noranizan; Abdul Rahman, Russly; Zainal Abedin, Nur Hanani; Hussain, Norhayati; Sulaiman, Rabiha; Chong, Gun Hean

    2018-02-11

    Recent rapid growth of the world's population has increased food demands. This phenomenon poses a great challenge for food manufacturers in maximizing the existing food or plant resources. Nowadays, the recovery of health benefit bioactive compounds from fruit wastes is a research trend not only to help minimize the waste burden, but also to meet the intensive demand from the public for phenolic compounds which are believed to have protective effects against chronic diseases. This review is focused on polyphenolic compounds recovery from tropical fruit wastes and its current trend of utilization. The tropical fruit wastes include in discussion are durian (Durio zibethinus), mangosteen (Garcinia mangostana L.), rambutan (Nephelium lappaceum), mango (Mangifera indica L.), jackfruit (Artocarpus heterophyllus), papaya (Carica papaya), passion fruit (Passiflora edulis), dragon fruit (Hylocereus spp), and pineapple (Ananas comosus). Highlights of bioactive compounds in different parts of a tropical fruit are targeted primarily for food industries as pragmatic references to create novel innovative health enhancement food products. This information is intended to inspire further research ideas in areas that are still under-explored and for food processing manufacturers who would like to minimize wastes as the norm of present day industry (design) objective.

  9. ArtinM offers new perspectives in the development of antifungal therapy

    Directory of Open Access Journals (Sweden)

    Luciana Pereira Ruas

    2012-06-01

    Full Text Available The thermally dimorphic fungus Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis (PCM, the most frequent systemic mycosis that affects the rural populations in Latin America. Despite significant developments in antifungal chemotherapy, its efficacy remains limited since drug therapy is prolonged and associated with toxic side effects and relapses. In response to these challenges, it is now recognized that several aspects of antifungal immunity can be modulated to better deal with fungal infections. A common idea for halting fungal infections has been the need to activate a cell-based, pro-inflammatory Th1 immune response to improve the fungal elimination. ArtinM, a D-mannose binding lectin from Artocarpus heterophyllus, has the property of modulating immunity against several intracellular pathogens. Here, we review the immunomodulatory activity of ArtinM during experimental PCM in mice. Both prophylactic and therapeutic protocols of ArtinM administration promotes a Th1 immune response balanced by IL-10, which outstandingly reduces the fungal load in organs of the treated mice while maintaining a controlled inflammation at the site of infection. A carbohydrate recognition based interaction of ArtinM

  10. Community-wise evaluation of rice beer prepared by some ethnic tribes of Tripura

    Directory of Open Access Journals (Sweden)

    Sushanta Ghosh

    2016-12-01

    Full Text Available Tripura is inhabited by many indigenous communities having unique ethno-socio-cultural lifestyle with age-old rice beer brewing techniques using wild herbs and local rice varieties popularly known as chuwak or zu. The present study is focused on comparative evaluation of brewing methods and nutritional aspects of rice beer among Debbarma, Jamatia, Koloi, and Molsom tribes of Tripura. Sample ingredients and plant species are properly identified before reporting. Rice beer is also prepared in laboratory conditions for comparative studies of qualitative and quantitative aspects. Thirteen different plant species are used by these four tribes for preparation of starter cultures using soaked rice flour. Markhamia stipulate (Wall. Seem. is common to all communities for starter cake preparation. Litsea monopetala (Roxb. Pers. is used by all three communities except Jamatia. The use of Ananus comosus Mill. is common among Debbarma and Jamatia tribes, whereas that of Artocarpus heterophyllus Lam. is common among Molsom and Koloi tribes. However, Aporusa diocia (Roxb. Muell., Combretum indicum (L. DeFilipps., and Citrus sinensis (L. Osbeck. are used only by Debbarma tribe for unique tangy flavor. The physicochemical properties of rice beer varied within tribes for its moisture content, carbohydrate content, reducing sugar, and alcohol percentage. The concentration of alcohol increases with aging and prolonged fermentation. The plants reported here are also reported for having nutritional and medicinal benefits for the metabolic stability in humans, which make the process more prospective for commercialization if a standard for maintaining a quality and associated risk can be determined.

  11. Application of dietary fiber method AOAC 2011.25 in fruit and comparison with AOAC 991.43 method.

    Science.gov (United States)

    Tobaruela, Eric de C; Santos, Aline de O; Almeida-Muradian, Ligia B de; Araujo, Elias da S; Lajolo, Franco M; Menezes, Elizabete W

    2018-01-01

    AOAC 2011.25 method enables the quantification of most of the dietary fiber (DF) components according to the definition proposed by Codex Alimentarius. This study aimed to compare the DF content in fruits analyzed by the AOAC 2011.25 and AOAC 991.43 methods. Plums (Prunus salicina), atemoyas (Annona x atemoya), jackfruits (Artocarpus heterophyllus), and mature coconuts (Cocos nucifera) from different Brazilian regions (3 lots/fruit) were analyzed for DF, resistant starch, and fructans contents. The AOAC 2011.25 method was evaluated for precision, accuracy, and linearity in different food matrices and carbohydrate standards. The DF contents of plums, atemoyas, and jackfruits obtained by AOAC 2011.25 was higher than those obtained by AOAC 991.43 due to the presence of fructans. The DF content of mature coconuts obtained by the same methods did not present a significant difference. The AOAC 2011.25 method is recommended for fruits with considerable fructans content because it achieves more accurate values. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. THE POTENTIAL OF VEGETATION SPECIES DIVERSITY FOR ECOTOROURISM DEVELOPMENT AT NATURE RESERVE OF PANJALU LAKE

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    Encep Rahman

    2017-06-01

    Full Text Available The Nature Reserve of Panjalu Lake is one of the oldest conservation area in Indonesia. As a conservation area, Panjalu Lake has different species of flora that are useful as germplasm conservation, science and education. This study aims to know the potential of vegetation species diversity for ecotourism development at Nature Reserve of Panjalu Lake. The inventory method used is line plot sampling with intensity 15 % in two paths of 500 m (adjusted according length of the area and 20 m width. Spacing between lines is 200 m and spacing between observation plot is 100 m. Within each path, 50 m x 20 m observation plots were established. The results showed that there are three species of seedlings with highest IVI, namely: Dysoxylum densiflorum Miq. (47.64 %, Calamus zollingerii (47.64 %, and Sterculia macrophylla Vent. (44.37 %. The four species at sapling stage with highest IVI are: Litsea cassiaefolia (114.29 %; Dysoxylum densiflorum Miq (57.14 %; Litsea sp. and Endiandra rubescens Miq (14.29 %. Three species at pole stage with highest IVI, namely: Dysoxylum densiflorum Miq. (143.04%; Litsea cassiaefolia (99.78 % and Artocarpus elasticus Reinw 9.53 %. Three species at tree stage with highest IVI, namely: Dysoxylum densiflorum Miq (147.924 %, Litsea cassiaefolia (68.753 %, and Eugenia fastigiata Miq ( 31.410 %.

  13. Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy

    Science.gov (United States)

    Poiroux, Guillaume; Barre, Annick; van Damme, Els J. M.; Benoist, Hervé; Rougé, Pierre

    2017-01-01

    Aberrant O-glycans expressed at the surface of cancer cells consist of membrane-tethered glycoproteins (T and Tn antigens) and glycolipids (Lewis a, Lewis x and Forssman antigens). All of these O-glycans have been identified as glyco-markers of interest for the diagnosis and the prognosis of cancer diseases. These epitopes are specifically detected using T/Tn-specific lectins isolated from various plants such as jacalin from Artocarpus integrifola, and fungi such as the Agaricus bisporus lectin. These lectins accommodate T/Tn antigens at the monosaccharide-binding site; residues located in the surrounding extended binding-site of the lectins often participate in the binding of more extended epitopes. Depending on the shape and size of the extended carbohydrate-binding site, their fine sugar-binding specificity towards complex O-glycans readily differs from one lectin to another, resulting in a great diversity in their sugar-recognition capacity. T/Tn-specific lectins have been extensively used for the histochemical detection of cancer cells in biopsies and for the follow up of the cancer progression and evolution. T/Tn-specific lectins also induce a caspase-dependent apoptosis in cancer cells, often associated with a more or less severe inhibition of proliferation. Moreover, they provide another potential source of molecules adapted to the building of photosensitizer-conjugates allowing a specific targeting to cancer cells, for the photodynamic treatment of tumors. PMID:28598369

  14. Plant latex: a promising antifungal agent for post harvest disease control.

    Science.gov (United States)

    Sibi, G; Wadhavan, Rashmi; Singh, Sneha; Shukla, Abhilasha; Dhananjaya, K; Ravikumar, K R; Mallesha, H

    2013-12-01

    Bioactive compounds from plant latex are potential source of antifungic against post harvest pathogens. Latex from a total of seven plant species was investigated for its phytochemical and antifungal properties. Six fungi namely Aspergillus fumigatus, A. niger, A. terreus, F. solani, P. digitatum and R. arrhizus were isolated from infected fruits and vegetables and tested against various solvent extracts of latex. Analysis of latex extracts with phytochemical tests showed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids, tannins and terpenoids. Antifungal assay revealed the potential inhibitory activity of petroleum ether extracts against the postharvest fungal isolates. Various degree of sensitivity was observed irrespective of plant species studied with A. terreus and P. digitatum as the most susceptible ones. F. solani and A. fumigatus were moderately sensitive to the latex extracts tested. Among the plants, latex of Thevetia peruviana (75.2%) and Artocarpus heterophyllus (64.8%) were having potential antifungal activity against the isolates followed by Manilkara zapota (51.1%). In conclusion, use of plant latex makes interest to control postharvest fungal diseases and is fitting well with the concept of safety for human health and environment.

  15. Pengaruh penggunaan bahan pewarna alam dari ekstrak kayu terhadap sifat fisis kulit ikan kakap merah

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    Emiliana Kasmudjiastuti

    2002-06-01

    Full Text Available Abstract The objective of this research is to identify the effect of some natural dye of wood extract to the physical properties (tensile strength, elongation and rub fastness resistance of kakap (Lutjanidae fish skin leather. Fifteen pieces of dry preserved kakap fish skin were used in this research, and tanned using syntan and alum. The dyeing process was done using natural dyes of wood extract of Caesalpinia sappan L, Artocarpus heterophyllus, Swietenia mahagoni JACQ, Maclura cochinensis, and Ceriops tagal. The result of research indicated that the kind of applied natural dyes were not affect the physical properties of Kakap fish skins. The fish skins dyed using the such natural dyes was able be utilised as material in leather goods manufacturing. One of the five types of natural dye used in this research, Swietenia mahagoni JACQ wood extract produced the best dyed leather having good tensile strength, elongation/flexibility and the colour fastness. The tensile strength was 213,15 kg/cm2, flexibility 56% and colour fastness with wet white cloth 3/4 (grey scale value and with dry white cloth 4/5 (grey scale value, respectively

  16. Antityrosinase and antimicrobial activities from Thai medicinal plants.

    Science.gov (United States)

    Dej-Adisai, Sukanya; Meechai, Imron; Puripattanavong, Jindaporn; Kummee, Sopa

    2014-04-01

    Various dermatological disorders and microbial skin infection can cause hyperpigmentation. Therefore, screenings for whitening and antimicrobial agents from Thai medicinal plants have been of research interest. Seventy-seven ethanol plant extracts were investigated for antityrosinase activity, eleven samples showed the tyrosinase inhibition more than 50 % were further preliminary screening for antimicrobial activity by agar disc diffusion and broth micro-dilution methods. Artocarpus integer (Thunb.) Merr. (Moraceae) root extract, which showed the potential of tyrosinase inhibition with 90.57 ± 2.93 % and antimicrobial activity against Staphylococcus aureus, S. epidermidis, Propionibacterium acnes and Trichophyton mentagophytes with inhibition zone as 9.10 ± 0.00, 10.67 ± 0.09, 15.25 ± 0.05 and 6.60 ± 0.17 mm, respectively was selected for phytochemical investigation. Three pure compounds were isolated as artocarpin, cudraflavone C and artocarpanone. And artocarpanone exhibited anti-tyrosinase effect; artocarpin and cudraflavone C also showed the potential of antibacterial activity against S. aureus, S. epidermidis and P. acnes with MIC at 2, 4 and 2 μg/ml, respectively and MBC at 32 μg/ml for these bacteria. So, these pure compounds are interesting for further study in order to provide possibilities of new whitening and antibacterial development. This will be the first report of phytochemical investigation of A. integer root.

  17. Phytosociology of aquilaria malaccensis lamk. and its communities from a tropical forest reserve in peninsular malaysia

    International Nuclear Information System (INIS)

    Razak, S. A.; Haron, N. W.

    2015-01-01

    A phytosociological study on the floristic and vegetation communities of Aquilaria malaccensis was carried out in Sungai Udang Forest Reserve, Malacca, Malaysia. The main objectives of this study were to identify, characterize and classify the Aquilaria malaccensis communities which were naturally distributed in the Sungai Udang Forest Reserve. A total of 25 plots (40 m x 20 m) in size were constructed according to the line transect method. The vegetation sampling and data analysis were done. A total of 80 species belonging to 79 genera and 40 families were found from all the 25 plots in Sungai Udang Forest Reserve. The most abundant family was the Euphorbiaceae with 220 individual trees, followed by Myrtaceae and Anacardiaceae representing 212 and 197 individual trees, respectively. A community which was Aquilaria malaccensis Artocarpus rigidus community with two new sub-community known as Palaquium gutta sub-community and Barringtonia racemosa sub-community were identified on the basis of statistical and phytosociological analyses. These community and sub-community also showed preference on different geographical and environmental factors such as soils and local relief. This study is useful in providing more information on the growth response of the mixed dipterocarp forest in the development of proper forest management. (author)

  18. Breadnut peel as a highly effective low-cost biosorbent for methylene blue: Equilibrium, thermodynamic and kinetic studies

    Directory of Open Access Journals (Sweden)

    Linda B.L. Lim

    2017-05-01

    Full Text Available This work reports the potential use of peel of breadnut, Artocarpus camansi, as an effective low-cost biosorbent for the removal of methylene blue (MB. Oven dried A. camansi peel (ACP, which had a point of zero charge at pH = 4.8, showed maximum biosorption capacity which was far superior to most literature reported fruit biomasses, including samples that have been activated. Isotherm studies on biosorption of MB onto ACP gave a maximum biosorption capacity of 409 mg g−1. The Langmuir model was found to give the best fit among various isotherm models investigated and error analyses performed. Kinetics studies were fast with 50% dye being removed in less than 8 min from a 50 mg L−1 dye solution and further, kinetics followed the pseudo second order. Thermodynamic studies indicated that the biosorption process was both spontaneous and exothermic. Fourier transform infrared (FT-IR of ACP before and after MB adsorption was investigated. It can be concluded that oven dried breadnut peel is a highly promising low-cost biosorbent with great potential for the removal of MB.

  19. Expression of antigen tf and galectin-3 in fibroadenoma.

    Science.gov (United States)

    Gallegos, Itandehui Belem; Pérez-Campos, Eduardo; Martinez, Margarito; Mayoral, Miguel Ángel; Pérez, Laura; Aguilar, Sergio; Zenteno, Edgar; Pina, Maria del Socorro; Hernández, Pedro

    2012-12-24

    Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors. Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts. Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

  20. Expression of antigen tf and galectin-3 in fibroadenoma

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    Gallegos Itandehui Belem

    2012-12-01

    Full Text Available Abstract Background Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors. Findings Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts. Conclusions Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

  1. Antilisterial effects of ethanolic extracts of some edible Thai plants on refrigerated cooked pork

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    Siriporn Stonsaovapak

    2010-12-01

    Full Text Available Listeria monocytogenes is a major foodborne pathogen responsible for the disease listeriosis.Effective methods for reducing L. monocytogenes in foods would reduce the likelihood of foodborneoutbreaks of listeriosis and decrease economic losses to the food industry. Crude ethanolic extracts from 50 edible Thai plants were screened for inhibitory effects on isolated strains and type strains of L.monocytogenes by the well assay technique. Ethanolic extracts of Micromelum minutum, Artocarpus heterophyllus, Piper retrofractum and Cucurbita moschata, which showed listerial growth inhibition,were applied to cooked pork to determine their antimicrobial activities against L. monocytogenes. Pork was cooked to an internal temperature of 85C, allowed to cool to 8C and then treated by surface application with the plant extracts. Low (102 cfu g-1 or high (105 cfu g-1 population of L.monocytogenes were applied and samples were stored at 4C for up to 7 days. M. minutum and A.heterophyllus extracts were most effective in inhibiting the growth of the pathogen. These results suggested that some edible Thai plant extracts might be useful as antimicrobials in cooked, ready-to-eatpork.

  2. DIVERSITY OF PLANT COMMUNITIES IN SECONDARY SUCCESSION OF IMPERATA GRASSLANDS IN SAMBOJA LESTARI, EAST KALIMANTAN, INDONESIA

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    Ishak Yassir

    2016-06-01

    Full Text Available Regeneration of  Imperata grassland areas is becoming increasingly important, both to create new secondary forest and to recover the original biodiversity. The diversity of  plant communities in secondary succession of  Imperata grasslands was studied using 45 subplots of  9 linear transects (10 m x 100 m. Data was collected and all stems over 10 cm dbh were identified, the Importance Values Index (IVI for all trees were calculated, saplings and seedlings were counted  and analysed, and soil samples were taken and analysed. Results showed that  after more than 10 years of  regeneration, 65 families were encountered consisting of  164 species, which were dominated by Vernonia arborea Buch.-Ham, Vitex pinnata L., Macaranga gigantea (Reichb.f. & Zoll. Muell.Arg., Symplocos crassipes C.B. Clarke, Artocarpus odoratissimus Miq., and Bridelia glauca Blume. The effects of  regeneration, from Imperata grassland to secondary forest, on soil were the strongest in the A-horizon where an increase in carbon, N content, and pH were observed. Our result shows that Imperata grasslands appear to be permanent because of  frequent fires and human interferences and so far few efforts have been made to promote sustainable rehabilitation. If  protected from fire and other disturbances, such as shifting cultivation, Imperata grassland will grow and develop into secondary forest.

  3. Alternative energy sources from plants of Western Ghats (Tamil Nadu, India)

    Energy Technology Data Exchange (ETDEWEB)

    Augustus, G.D.P.S.; Jayabalan, M. [V.H.N.S.N College, Virudhunagar (India). Research Centre in Botany; Seiler, G.J. [USDA-ARS, Northern Crop Science Lab., Fargo, ND (United States)

    2003-06-01

    Twenty-two taxa of Western Ghats plants were screened as potential alternative crops for renewable energy, oil, hydrocarbon and phytochemicals. The highest hydrocarbon yields were observed in Carissa carandas (1.7%), and Jatropha gossypifolia (1.7%). The highest polyphenol fraction was observed in Dodonaea viscosa (17.1%), Carissa carandas (7.7%), Swietenia mahagoni (6.6%), and Jatropha glandulifera (6.2%). The highest oil content was observed in Aganosma cymosa (10.3%), Carissa carandas (5.8%), and Argemone mexicana (5.0%). Swietenia mahagoni yielded the highest protein content with 8.1%. The gross heat value of 4175.0 cal/g(17.5 MJ/kg) for Lochnera rosea (pink flowered var.), and 4112.0 cal/g for Dalbergia sissoo were the highest among the species analysed. NMR spectra of the hydrocarbon fractions of Alstonia scholaris, Carissa carandas, Ichnocarpus frutescens, Plumeria rubra, Thevetia neriifolia (white flowered var.), Vallaris solanacea, Lochnera rosea (pink flowered var.), Euphorbia hirta, E. splendens, Artocarpus integrifolia and Ficus religiosa revealed the presence of cis-polyisoprene (natural rubber), whereas Argemone mexicana showed the presence of trans-polyisoprene (gutta). Several new crop species were identified with potentially useful compounds. The potential exists for growing these alternate crops in areas of under-utilized lands, subsequently stimulating industrial and economic growth. (author)

  4. Community stand structure of rehabilitated forest at Kenaboi Forest Reserve, Negeri Sembilan, Malaysia

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    Fatma, N. A. H.; Wan Juliana, W. A.; Shaharuddin, M. I.; Wickneswari, R.

    2016-11-01

    A descriptive study of species composition, community structure and biomass was conducted in compartment 107, which is a rehabilitated area at Kenaboi Forest Reserve, Jelebu, Negeri Sembilan. The objective is to determine the forest structure and species composition in a rehabilitated area of Kenaboi FR since enrichment planting had done. A sample plot of 1 hectare was censused and a total of 395 trees with diameter ≥ 5 cm DBH were recorded. A total of 285 individual trees were identified belonging to 20 families and the commonest family was Dipterocarpaceae with 193 individuals. The highest tree density per ha was Shorea acuminata at 33% followed by S. parvifolia, 10% and S. leprosula, 6%. The biggest tree was Artocarpus elasticus Reinw. ex Blume with a diameter of 101 cm. The total basal area was 34.48 m2/ha, whereby the highest basal area was between 45 - 54.9 cm DBH class that contributed 10.21 m2/ha (30%). The total biomass estimation (above ground and below ground) was 792.57 t/ha. Dipterocarpaceae contributed the highest total biomass at 545.14 t/ha with S. acuminata contributed the highest total biomass of 330.45 t/ha. This study will contribute to the knowledge of regeneration forest especially on how the ecological process restoring the biodiversity and ecosystem functioning in rehabilitated forest by practicing the enrichment planting of native species.

  5. Fruit of plant diversity at home-garden of Jabon Mekar village, subdistrict of Parung, Bogor regency

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    BUDI PRASETYO

    2007-01-01

    Full Text Available Jabon Mekar village is well-known as the central of fruit supplayer at subdistrict of Parung. Many kinds of fruit were planted and developed in this area. Durian ‘lai-mas’ or durian jabon’s cultivar is the superior product of fruit. However it was estimated as a buffer zone of Jakarta and subject of the urban development. Due to the increasing number of the urbant development in Jakarta, it is concerned that this will have an effect to the function of home-garden.The aim of the research is to study the potential riches and fruit plant diversity at home-garden of community in the village of Jabon Mekar. The methods used for vegetation analysis were the quadratic method. The result of the research found 57 species of fruit plants from 41 genus, 23 families and 30 local cultivars. From all fruit of plants, there are 7 species as the main compositer of the community at home-garden i.e. Musa sp., Durio zibethinus, Nephelium lappaceum, Cocos nucifera, Artocarpus heterophyllus, Sandoricum koetjape, Carica papaya. It was found also that the diversity of plant species at home-garden was at the high level. While all fruit of plant species found a tendency SDR value under 50%, means that none of the plant species dominant toward other fruit of plants species.

  6. Nilai Kekuatan Tumpu Baut pada Empat Jenis Kayu Rakyat Indonesia

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    Sucahyo Sadiyo

    2017-08-01

    Full Text Available The fasteners bearing strength is one of the properties that affect reference design value (Z on dowel-type connections. In Indonesia, the value of bolt bearing strength can be determined in theory by SNI 7973 which adopt NDS standard. This study was conducted to determine the bolt bearing strength by using three bolts diameter (1/2 in, 5/8 in and 3/4 in and four Indonesian woods community species which are sengon (Paraserianthes falcataria, jabon (Anthocephalus cadamba, manii (Maesopsis eminii and nangka (Artocarpus heterophyllus compared with SNI 7973. The results showed that bolt bearing strength can be affected by density and specific gravity. Moreover, the compression strength parallel to grain can be used as a parameter to predict bolt bearing strength. The higher compression strength parallel to grain, the higher bolt bearing strength will be. The bolt bearing strength based on wood species were sengon 161 kg/cm2, jabon 151 kg/cm2, manii 243 kg/cm2 and nangka 392 kg/cm2 respectively. Bolt with 1/2 in diameter has the highest bolt bearing strength except for jabon wood. The difference of bolt bearing strength on sengon and jabon versus SNI 7973 was differ significantly by 16-27% while the bolt bearing strength on manii and nangka was not differ significantly by 2-3%.

  7. Faba Greens, Globe Artichoke’s Offshoots, Crenate Broomrape and Summer Squash Greens: Unconventional Vegetables of Puglia (Southern Italy With Good Quality Traits

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    Massimiliano Renna

    2018-03-01

    Full Text Available Globe artichoke (Cynara cardunculus L. subsp. [L.] scolymus Hayek, summer squash (Cucurbita pepo L. and faba bean (Vicia faba L. are widely cultivated for their immature inflorescences, fruits and seeds, respectively. Nevertheless, in some areas of Puglia (Southern Italy, other organs of these species are traditionally used as vegetables, instead of being considered as by-products. Offshoots (so-called cardoni or carducci of globe artichoke, produced during the vegetative growing cycle and removed by common cultural procedures, are used like to the cultivated cardoons (C. cardunculus L. var. altilis DC. The stems, petioles, flowers and smaller leaves of summer squash are used as greens (so-called cime di zucchini, like other leafy vegetables such as chicory (Cichorium intybus L. and Swiss chard (Beta vulgaris L.. Also the plant apex of faba bean, about 5–10 cm long, obtained from the green pruning, are used as greens (so-called cime di fava like spinach leaves. Moreover, crenate broomrape (Orobanche crenata Forssk., a root parasite plant that produces devastating effects on many crops (mostly legumes, is used like asparagus (Asparagus officinalis L. to prepare several traditional dishes. In this study ethnobotanical surveys and quality assessment of these unconventional vegetables were performed. For their content of fiber, offshoots of globe artichokes can be considered a useful food to bowel. Summer squash greens could be recommended as a vegetable to use especially in the case of hypoglycemic diets considering both content and composition of their carbohydrates. For their low content of nitrate, faba greens could be recommended as a substitute of nitrate-rich leafy vegetables. Crenate broomrape shows a high antioxidant activity and may be considered as a very nutritious agri-food product. Overall, the results of the present study indicate that offshoots of globe artichoke, summer squash greens, faba greens and crenate broomrape have good

  8. Necessity of Aloha in Humanity s travels from the Earth

    Science.gov (United States)

    Yuen, D.

    While technology and hardware provide the physical means to reach the Moon and beyond, the greatest explorers in human history took not only technologies, but a strong and guiding faith. This poster presentation seeks to present to the conference attendees the desirablility of using, as a m del for exploration, the Polynesiano voyaging culture, and most importantly, the concept of "Aloha" as a sound basis for interaction between humans of various cultures, and as a platform on which to base future exploration. The concept of "Aloha" is t h e c rnerstone of Hawaiian culture. It assures theo travelers' welcome, and it assures a safe departure. It is a haven in time of storm, and a fair wind along the journey. It is the food and drink of the soul. As we continue our explorations to new worlds, it is paramount that we continue the tradition of Aloha. The ancestors of the Hawaiians were adventurers, explorers, and settlers. Not knowing if they would be able to return to the homeland, they took with them the necessities of their lives - pigs, chickens, dogs, taro, sweet potato, breadfruit, gourds - and many other things they knew to be useful. And they took Aloha. We also must take with us food and tools, though in different forms than the early explorers. And, we also must take Aloha. Aloha is similar in concept to Namaste, a Sanskrit greeting which can be translated as "the divine in me honors the divine in you." It is far more rich in meaning than can be expressed in a Visitors' Bureau phrasebook. While Aloha is translated as, "hello," "goodby," "love," "affection," and "friendship," it also embodies responsibility for oneself and for one's actions toward others. It is a verbal shorthand which expresses an acknowledgement of the net-like interweaving of family and community relationships. In the twenty first century we can look to the example of these successful explorers. We, too, can carry with us an inexhaustible supply of Aloha. It has no mass, no height, depth

  9. Population pressure on coral atolls: trends and approaching limits.

    Science.gov (United States)

    Rapaport, M

    1990-09-01

    Trends and approaching limits of population pressure on coral atolls is discussed by examining the atoll environment in terms of the physical geography, the production systems, and resource distribution. Atoll populations are grouped as dependent and independent, and demographic trends in population growth, migraiton, urbanization, and political dependency are reviewed. Examination of the carrying capacity includes a dynamic model, the influences of the West, and philopsophical considerations. The carrying capacity is the "maximal population supportable in a given area". Traditional models are criticized because of a lack in accounting for external linkages. The proposed model is dynamic and considers perceived needs and overseas linkages. It also explains regional disparities in population distribution, and provides a continuing model for population movement from outer islands to district centers and mainland areas. Because of increased expectations and perceived needs, there is a lower carrying capacity for outlying areas, and expanded capacity in district centers. This leads to urbanization, emigration, and carrying capacity overshot in regional and mainland areas. Policy intervention is necessary at the regional and island community level. Atolls, which are islands surrounding deep lagoons, exist in archipelagoes across the oceans, and are rich in aquatic life. The balance in this small land area with a vulnerable ecosystem may be easily disturbed by scarce water supplies, barren soils, rising sea levels in the future, hurricanes, and tsunamis. Traditionally, fisheries and horticulture (pit-taro, coconuts, and breadfruit) have sustained populations, but modern influences such as blasting, reef mining, new industrial technologies, population pressure, and urbanization threaten the balance. Population pressure, which has lead to pollution, epidemics, malnutrition, crime, social disintegration, and foreign dependence, is evidenced in the areas of Tuvalu, Kiribati

  10. Study on NPK Performance in Food Waste Composting by Using Agricultural Fermentation

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    Jamaludin Siti Noratifah

    2017-01-01

    Full Text Available Food waste represents almost 60% from the total municipal solid waste disposed in the landfill. Alternative disposal method for food waste could be conducted by using composting method. In this study, investigationon food waste composting by using agricultural fermentation was carried out to find out the performance of the compost. Two types of compost were produced which were commercial compost and research compost and total of 8 reactors were tested during this study. Research compost consist of coconut fiber (decomposing medium and the combination of salt and breadfruit peels as the fermentation liquid, while rice husk was used as decomposing medium for commercial compost along with fermented soybeanand brown sugar as fermentation liquid. Physical and chemical parameters which are temperature, pH value, moisture content, Total Nitrogen (N, Total Phosphorus (P and Potassium (K concentration were determined. Based on the results of 20 weeks composting, the overall temperature range from 27 °C to 45 °C which shown the active phase for composting occurred. On the other hand, during the period of composting, most of the pH value in each reactor is above 5 and approximately at neutral. This shown that the microbial respiration in the composting reactor was inhibited and had approached the mature phase. As for NPK content, Total Nitrogen value range from 98 ppm to 2268 ppm for commercial compost, while 84 ppm to 2240 ppm for research compost. Total Phosphorus has the values of0.871 ppm to 11.615 ppm for commercial compost and 1.785 ppm to 14.143 ppm for research compost. On the other hand, result for potassium is from 91.85 ppm to 645.55 ppm for commercial compost and from 133.95 ppm to 686.2 ppm for research compost. As a conclusion from the results obtained, the compost in this study is sufficient to be use for agricultural purposes and the best performance of NPK value was demonstrated by Reactor C2 from research compost.

  11. Women in Guam consume more calories during feast days than during non-feast days.

    Science.gov (United States)

    Paulino, Yvette C; Leon Guerrero, Rachael T; Novotny, Rachel

    2011-03-01

    Parties (feast days) have become increasingly frequent and abundant, in terms of food, on the island of Guam. Considering the potential impact of this frequency and abundance on food intake, this study compared food intake during feast days and non-feast days of women in Guam (25 Chamorros; 24 Filipinas). The women (≥40 yr) recalled foods they usually consumed during feast days. Subsequently, a 24-hour dietary recall for a non-feast day was completed by a subsample (n=25). Height, weight, and waist circumference were measured to assess obesity status. Statistical analyses (paired t-test, ANOVA, and chi-square test) were performed with SPSS. Compared to a non-feast day, the women reported higher intakes of dietary energy (2645.0 ± 1125.8 versus 1654.0 ± 718.8 kcal/day), carbohydrates (43.8 ± 11.5% versus 51.8 ± 10.2% of kcal), total fat (34.1 ± 7.8% versus 27.5 ± 9.6% of kcal), saturated fat (11.4 ± 4.7% versus 7.9 ± 3.4% of kcal), and sugar (89.5 ± 62.8 versus 47.3 ± 42.2 g/day) on feast days. Chamorros, compared to Filipinas, reported higher dietary energy density (1.7 ± 0.4 versus 1.4 ± 0.3 kcal/g), total fat (35.3 ± 8.9% versus 30.7 ± 6.8% of kcal), and saturated fat (12.4 ± 4.9% versus 9.4 ± 3.3% of kcal); and lower servings of fruit (0.5 ± 1.0 versus 2.7 ± 1.8) on feast days. Fourteen Chamorros (56.0%) and one Filipina (4.1%) were classified as obese. Current feasting behaviors of women in Guam may contribute to obesity if continued for a long period. The women would benefit by choosing more fruit and vegetable dishes in place of high-energy dishes. Chamorro women would particularly benefit by reducing saturated fat intake. Traditional foods, such as taro, breadfruit, seafood, fruits, and vegetables, would help accomplish this and thus should be promoted at parties on Guam.

  12. Tolerance to water deficit in young trees of jackfruit and sugar apple Tolerância ao déficit hídrico em plantas jovens de jaqueira e pinheira

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    Bruno Monteiro Rodrigues

    2010-06-01

    Full Text Available The predawn leaf water potential (Ψl, stomatal conductance (g s, CO2 assimilation (A, transpiration (E, chlorophyll a fluorescence and leaf metabolite contents (soluble sugars, proteins and free amino acids of two tropical fruit species grown in a greenhouse were evaluated to determine the effect of induced water stress on young plants. Six month-old jackfruit (Artocarpus heterophyllus Lam. and sugar apple (Annona squamosa L. plants were evaluated in 10.0 L pots after eight days of water withholding, imposed by suspension of irrigation. Jackfruit water status was better than sugar apple. Sugar apple plants showed different daily stomatal behavior when well hydrated, compared to jackfruit plants under the same conditions. The gas exchange of both species showed sensibility to high vapor pressure deficit (VPD. However, under water deficit, photochemical efficiency dropped at midday in both treatments (control and water deficit and both species showed low tolerance to high VPD and light intensity under greenhouse conditions. In contrast, some chlorophyll fluorescence variables showed good photosystem II stability at highest VPD hour (14:00 h. The present results involving carbohydrate metabolic changes revealed an accumulation of soluble sugars; moreover, protein and free amino acid contents in water stressed leaves also increased. These findings suggest an absence of damage to photosynthetic machinery for water deficit period. Jackfruit revealed greater tolerance to water deficit than sugar apple an important feature for commercial crops in northeastern Brazil.A condutância estomática (g s, a assimilação de CO2 (A, a transpiração (E, a fluorescência da clorofila e o conteúdo foliar de (açúcares solúveis, proteínas e aminoácidos livres de duas espécies frutíferas tropicais foram avaliados para determinar o efeito do déficit hídrico induzido sobre as plantas da jaqueira (Artocarpus heterophyllus Lam. e da pinheira (Annona squamosa

  13. ArtinM Mediates Murine T Cell Activation and Induces Cell Death in Jurkat Human Leukemic T Cells

    Science.gov (United States)

    Oliveira-Brito, Patrícia Kellen Martins; Gonçalves, Thiago Eleutério; Vendruscolo, Patrícia Edivânia; Roque-Barreira, Maria Cristina

    2017-01-01

    The recognition of cell surface glycans by lectins may be critical for the innate and adaptive immune responses. ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing TLR2 N-glycans and induces Th1 immunity. We recently demonstrated that ArtinM stimulated CD4+ T cells to produce proinflammatory cytokines. Here, we further studied the effects of ArtinM on adaptive immune cells. We showed that ArtinM activates murine CD4+ and CD8+ T cells, augmenting their positivity for CD25, CD69, and CD95 and showed higher interleukin (IL)-2 and interferon (IFN)-γ production. The CD4+ T cells exhibited increased T-bet expression in response to ArtinM, and IL-2 production by CD4+ and CD8+ T cells depended on the recognition of CD3εγ-chain glycans by ArtinM. The ArtinM effect on aberrantly-glycosylated neoplastic lymphocytes was studied in Jurkat T cells, in which ArtinM induced IL-2, IFN-γ, and IL-1β production, but decreased cell viability and growth. A higher frequency of AnnexinV- and propidium iodide-stained cells demonstrated the induction of Jurkat T cells apoptosis by ArtinM, and this apoptotic response was reduced by caspases and protein tyrosine kinase inhibitors. The ArtinM effects on murine T cells corroborated with the immunomodulatory property of lectin, whereas the promotion of Jurkat T cells apoptosis may reflect a potential applicability of ArtinM in novel strategies for treating lymphocytic leukemia. PMID:28665310

  14. Effect of ArtinM on Human Blood Cells During Infection With Paracoccidioides brasiliensis

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    Luciana P. Ruas

    2018-05-01

    Full Text Available Infections caused by fungi are prominent in our environment and can be potentially fatal. paracoccidioidomycosis (PCM, caused by fungi of the Paracoccidioides genus, is the most frequent systemic mycosis in Brazil and the main cause of death among immunocompetent individuals. The antifungal therapy for PCM is usually effective but side effects and relapses are often reported. The latter could be avoided with alternative or complementary therapies aimed at boosting the immune response to combat this pathogen. Recent reports have pointed at the importance of an effective cellular immune response, with the participation of Th1 cells, in the resistance to and control of Paracoccidioides infection. The ArtinM lectin, extracted from jackfruit (Artocarpus heterophyllus seeds, exhibits immunomodulatory activity against several intracellular pathogens, including Paracoccidioides brasiliensis, by promoting the development of a Th1 immune response. The aim of this work was to characterize the effect of ArtinM on peripheral blood cells of patients with PCM and on those of control individuals infected with fungal yeasts cells in vitro. Our results demonstrate that ArtinM activates human neutrophils in vitro, leading to an increase in cytokine production and CD54 expression. ArtinM activated P. brasiliensis-infected neutrophils from both healthy individuals and patients with PCM. This activation was not dependent on the dectin-1 receptor, because pre-incubation with laminarin, a dectin-1 receptor blocker, did not reverse the activated state of the cells. ArtinM also stimulated human peripheral blood mononuclear cells to secrete pro-inflammatory Th1-related cytokines, which are protective against Paracoccidioides infection. These data support the immunostimulatory action of ArtinM and encourage new studies using the lectin for the immunotherapy of PCM.

  15. Estrutura fitossociológica, diamétrica e hipsométrica da comunidade arbórea de um fragmento de floresta atlântica no Município de Moreno, Pernambuco, Brasil

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    Anderson Pedro Bernardina Batista

    2013-04-01

    Full Text Available Este trabalho teve como objetivo caracterizar a estrutura fitossociológica da comunidade arbórea da borda de um fragmento de floresta atlântica no município de Moreno, Pernambuco. O estudo foi desenvolvido em um fragmento de Mata Atlântica com área de aproximadamente 9 ha, pertencente à Fazenda Haras Casa Branca, localizado no município de Moreno, Pernambuco. Foram alocadas sistematicamente 10 parcelas de 10 x 25 m, equidistante em 20 m, distribuída em 2 linhas, a distância entre as linhas foi de 25 m, cada linha foi composta por 5 parcelas, totalizando 2500 m². As analises foram estabelecidas, com base no nível de inclusão com circunferência a altura do peito a 1,30 m do solo (CAP ≥ 15 cm e, os dados de altura (H através de estimativaspara o estudo fitossociológico. Para avaliar a diversidade florística em nível de espécie, utilizou-se o índice de Shannon (H’. No total, foram amostrados 247 indivíduos arbóreos, pertencentes a 23 famílias botânicas, 30 gêneros e 43 espécies. A Artocarpus heterophyllus Lam. e a Tapirira guianensis Aubl., foram as duas espécies que apresentaram o maior valor de importância respectivamente.  

  16. Recognition of TLR2 N-Glycans: Critical Role in ArtinM Immunomodulatory Activity

    Science.gov (United States)

    da Silva, Thiago Aparecido; Ruas, Luciana Pereira; Nohara, Lilian L.; de Almeida, Igor Correia; Roque-Barreira, Maria Cristina

    2014-01-01

    TLR2 plays a critical role in the protection against Paracoccidioides brasiliensis conferred by ArtinM administration. ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus, induces IL-12 production in macrophages and dendritic cells, which accounts for the T helper1 immunity that results from ArtinM administration. We examined the direct interaction of ArtinM with TLR2using HEK293A cells transfected with TLR2, alone or in combination with TLR1 or TLR6, together with accessory proteins. Stimulation with ArtinM induced NF-κB activation and interleukin (IL)-8 production in cells transfected with TLR2, TLR2/1, or TLR2/6. Murine macrophages that were stimulated with ArtinM had augmented TLR2 mRNA expression. Furthermore, pre-incubation of unstimulated macrophages with an anti-TLR2 antibody reduced the cell labeling with ArtinM. In addition, a microplate assay revealed that ArtinM bound to TLR2 molecules that had been captured by specific antibodies from a macrophages lysate. Notably,ArtinM binding to TLR2 was selectively inhibited when the lectin was pre-incubated with mannotriose. The biological relevance of the direct interaction of ArtinM with TLR2 glycans was assessed using macrophages from TLR2-KOmice, which produced significantly lower levels of IL-12 and IL-10 in response to ArtinM than macrophages from wild-type mice. Pre-treatment of murine macrophages with pharmacological inhibitors of signaling molecules demonstrated the involvement of p38 MAPK and JNK in the IL-12 production induced by ArtinM and the involvement ofPI3K in IL-10 production. Thus, ArtinM interacts directly with TLR2 or TLR2 heterodimers in a carbohydrate recognition-dependent manner and functions as a TLR2 agonist with immunomodulatory properties. PMID:24892697

  17. Inhibition of Hepatocarcinogenesis by ArtinM via Anti-proliferative and Pro-apoptotic Mechanisms.

    Science.gov (United States)

    Braz, Mariana M; Roque-Barreira, Maria Cristina; Ramalho, Fernando S; Oliveira, Carlos A; Augusto, Marlei J; Ramalho, Leandra N Z

    ArtinM is a d-mannose-binding lectin found in the seeds of Artocarpus heterophyllus (jackfruit) that interacts with N-glycans, that is associated with receptors on the surface of phagocytic cells and induces the production of inflammatory mediators. Some of them are especially important because they may be required for antitumor immune response. This study aimed to evaluate the effect of ArtinM on hepatocellular preneoplastic foci. Wistar rats received 50 mg/kg of diethyl-nitrosamine (DEN) intraperitoneal weekly for 12 weeks. From the 14th week, the treated animals received 50 μg/kg of ArtinM subcutaneous every 2 weeks until the 18th week, whereas control animals were injected with vehicle alone. Preneoplastic-related factors were estimated using histological, western blotting and RT-PCR analysis. In comparison to the groups exposed to DEN, the ArtinM-treated rats showed diminution of preneoplastic foci, decreased expression of proliferating cell nuclear antigen (PCNA), increased number of nuclear p21 and p27 stained cells, augmented number of apoptotic cells, increased expression of p53, p42/44 MAPK and p21 proteins, reduced cyclin D1 (CCND1) protein levels and increased expression of TNFα and IFNγ genes. No difference was observed in interleukin 12 (IL12) protein levels. These findings indicate that ArtinM may provide protection against hepatocarcinogenesis as a result of the induction of cell-cycle blockage and pro-apoptotic mechanisms. Copyright © 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  18. Neutrophils Contribute to the Protection Conferred by ArtinM against Intracellular Pathogens: A Study on Leishmania major.

    Science.gov (United States)

    Ricci-Azevedo, Rafael; Oliveira, Aline Ferreira; Conrado, Marina C A V; Carvalho, Fernanda Caroline; Roque-Barreira, Maria Cristina

    2016-04-01

    ArtinM, a D-mannose binding lectin from Artocarpus heterophyllus, has immunomodulatory activities through its interaction with N-glycans of immune cells, culminating with the establishment of T helper type 1 (Th1) immunity. This interaction protects mice against intracellular pathogens, including Leishmania major and Leishmania amazonensis. ArtinM induces neutrophils activation, which is known to account for both resistance to pathogens and host tissue injury. Although exacerbated inflammation was not observed in ArtinM-treated animals, assessment of neutrophil responses to ArtinM is required to envisage its possible application to design a novel immunomodulatory agent based on carbohydrate recognition. Herein, we focus on the mechanisms through which neutrophils contribute to ArtinM-induced protection against Leishmania, without exacerbating inflammation. For this purpose, human neutrophils treated with ArtinM and infected with Leishmania major were analyzed together with untreated and uninfected controls, based on their ability to eliminate the parasite, release cytokines, degranulate, produce reactive oxygen species (ROS), form neutrophil extracellular traps (NETs) and change life span. We demonstrate that ArtinM-stimulated neutrophils enhanced L. major clearance and at least duplicated tumor necrosis factor (TNF) and interleukin-1beta (IL-1β) release; otherwise, transforming growth factor-beta (TGF-β) production was reduced by half. Furthermore, ROS production and cell degranulation were augmented. The life span of ArtinM-stimulated neutrophils decreased and they did not form NETs when infected with L. major. We postulate that the enhanced leishmanicidal ability of ArtinM-stimulated neutrophils is due to augmented release of inflammatory cytokines, ROS production, and cell degranulation, whereas host tissue integrity is favored by their shortened life span and the absence of NET formation. Our results reinforce the idea that ArtinM may be considered an

  19. Neutrophils Contribute to the Protection Conferred by ArtinM against Intracellular Pathogens: A Study on Leishmania major.

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    Rafael Ricci-Azevedo

    2016-04-01

    Full Text Available ArtinM, a D-mannose binding lectin from Artocarpus heterophyllus, has immunomodulatory activities through its interaction with N-glycans of immune cells, culminating with the establishment of T helper type 1 (Th1 immunity. This interaction protects mice against intracellular pathogens, including Leishmania major and Leishmania amazonensis. ArtinM induces neutrophils activation, which is known to account for both resistance to pathogens and host tissue injury. Although exacerbated inflammation was not observed in ArtinM-treated animals, assessment of neutrophil responses to ArtinM is required to envisage its possible application to design a novel immunomodulatory agent based on carbohydrate recognition. Herein, we focus on the mechanisms through which neutrophils contribute to ArtinM-induced protection against Leishmania, without exacerbating inflammation. For this purpose, human neutrophils treated with ArtinM and infected with Leishmania major were analyzed together with untreated and uninfected controls, based on their ability to eliminate the parasite, release cytokines, degranulate, produce reactive oxygen species (ROS, form neutrophil extracellular traps (NETs and change life span. We demonstrate that ArtinM-stimulated neutrophils enhanced L. major clearance and at least duplicated tumor necrosis factor (TNF and interleukin-1beta (IL-1β release; otherwise, transforming growth factor-beta (TGF-β production was reduced by half. Furthermore, ROS production and cell degranulation were augmented. The life span of ArtinM-stimulated neutrophils decreased and they did not form NETs when infected with L. major. We postulate that the enhanced leishmanicidal ability of ArtinM-stimulated neutrophils is due to augmented release of inflammatory cytokines, ROS production, and cell degranulation, whereas host tissue integrity is favored by their shortened life span and the absence of NET formation. Our results reinforce the idea that ArtinM may be

  20. Impacts of floods on forest trees and their coping strategies in Bangladesh

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    Shukla Rani Basak

    2015-03-01

    Full Text Available During recent years, the Government of Bangladesh, Non-Government Organizations (NGOs, semi-government organizations, private organizations and individuals have established a large number of plantations under different programs viz. social forestry, agro-forestry and avenue plantations with indigenous and exotic tree species without considering their habit and habitats. Along with the indigenous species like Albizia procera, Albizia lebbeck, Mangifera indica, Azadirachta indica, Gmelina arborea, Trewia nudiflora and Artocapus heterophyllus and many exotic species e.g. Swietenia macrophylla, Albizia saman, Dalbergia sissoo, Eucalyptus camaldulensis, Acacia auriculiformis, Melia sempervirens, Acacia mangium etc. have been planted randomly. With increasing trend of climate-induced floods, millions of trees have been dying due to floods and water-logging. The most affected species are Dalbergia sissoo, Albizia saman, Acacia auriculiformis, Acacia mangium and Artocarpus heterophyllus etc. This situation has caused severe impacts on socio-economic conditions of Bangladesh. The impacts involved a significant loss in terms of investment, biodiversity and afforestation program. Little investigations have been conducted to find out the causes of the deaths and also to find out the suitable adaptation practices to reduce impacts of floods on trees. This synthesis focused on the impacts of floods on plantations and also assessed the potential role of traditional forest management practices in addressing the effects flooding on forests in Bangladesh. The study added important information and revealed knowledge gaps on the causes of large forest deaths. It also provided recommendations for policy on the establishment of frequent floods resilient tree crop plantations.

  1. Extraction and analysis of prebiotics from selected plants from southern Thailand

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    Santad Wichienchot

    2011-10-01

    Full Text Available Thirteen plants and their parts acquired from southern Thailand were investigated for their polysaccharide contentsand prebiotic properties. The fresh, ground samples were extracted with 50% and 95% ethanol and water at ambient and boilingtemperatures. The extracts were freeze-dried, digested with HCl buffer and -amylase, and indigestible polysaccharidecontents were determined. Base on extract yields and indigestible polysaccharide contents, ten samples were chosen aspotential sources of prebiotics. These included embryo, flesh and pericarp of palm fruit (Borrassus flabellifer L., skin, fleshand seed of jackfruit (Artocarpus heterophyllus Lam., flesh of rambutan (Nephelium lappaceum L., jampadah (Artocarpusinteger Merr., and young coconut (Cocos nucifera Linn., and okra pod (Hibiscus esculentus Linn.. Their extract yields (%dry wt were 26.54, 44.94, 51.69, 71.54, 59.43, 16.00, 55.73, 34.11, 22.66, and 12.39, respectively, and indigestible polysaccharidecontents (mg/g dry extract were 409.85, 334.87, 705.80, 689.08, 605.76, 403.44, 566.83, 542.56, 513.87, and 460.73, respectively.The amounts of oligosaccharide were 33.69, 47.20, 14.13, 0.00, 98.05, 29.35, 9.43, 2.40, 0.00, and 49.15 mg/g dry extract, respectively. Subsequently, five samples were chosen for further studies and possible commercial development based on theirextract yield, the amount and type of oligosaccharides, i.e. palm flesh, palm embryo, jackfruit flesh, jackfruit seed, and okrapod. Molecular weights of the polysaccharides from the five samples were 190-1,600 Daltons with a degree of polymerizationof 5-6.

  2. The Recognition of N-Glycans by the Lectin ArtinM Mediates Cell Death of a Human Myeloid Leukemia Cell Line

    Science.gov (United States)

    Carvalho, Fernanda Caroline; Soares, Sandro Gomes; Tamarozzi, Mirela Barros; Rego, Eduardo Magalhães; Roque-Barreira, Maria-Cristina

    2011-01-01

    ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus (jackfruit), interacts with N-glycosylated receptors on the surface of several cells of hematopoietic origin, triggering cell migration, degranulation, and cytokine release. Because malignant transformation is often associated with altered expression of cell surface glycans, we evaluated the interaction of ArtinM with human myelocytic leukemia cells and investigated cellular responses to lectin binding. The intensity of ArtinM binding varied across 3 leukemia cell lines: NB4>K562>U937. The binding, which was directly related to cell growth suppression, was inhibited in the presence of Manα1-3(Manα1-6)Manβ1, and was reverted in underglycosylated NB4 cells. ArtinM interaction with NB4 cells induced cell death (IC50 = 10 µg/mL), as indicated by cell surface exposure of phosphatidylserine and disruption of mitochondrial membrane potential unassociated with caspase activation or DNA fragmentation. Moreover, ArtinM treatment of NB4 cells strongly induced reactive oxygen species generation and autophagy, as indicated by the detection of acidic vesicular organelles in the treated cells. NB4 cell death was attributed to ArtinM recognition of the trimannosyl core of N-glycans containing a ß1,6-GlcNAc branch linked to α1,6-mannose. This modification correlated with higher levels of N-acetylglucosaminyltransferase V transcripts in NB4 cells than in K562 or U937 cells. Our results provide new insights into the potential of N-glycans containing a β1,6-GlcNAc branch linked to α1,6-mannose as a novel target for anti-leukemia treatment. PMID:22132163

  3. Activation of spleen cells by ArtinM may account for its immunomodulatory properties.

    Science.gov (United States)

    Silva, Thiago Aparecido da; Souza, Maria Aparecida de; Cecílio, Nerry Tatiana; Roque-Barreira, Maria Cristina

    2014-09-01

    ArtinM is a D-mannose-binding lectin extracted from Artocarpus heterophyllus that promotes interleukin-12 production by macrophages and dendritic cells. This property is considered responsible for T helper 1 immunity induced in vivo after ArtinM administration. In this study, we investigated the effect of native (jArtinM) and recombinant (rArtinM) forms of lectin on murine spleen cells and isolated T lymphocytes. We found that ArtinM binds to the surface of spleen cells. This interaction, which was blocked by D-mannose, induced cell activation, as manifested by increased mitochondrial activity, interleukin-2 production, and cell proliferation. We verified that a 30-times higher concentration of rArtinM was required to trigger optimal activation of spleen cells compared with that needed with jArtinM, although these proteins have identical sugar recognition properties and use the same signaling molecules to trigger cell activation. Because the distinction between native and recombinant is restricted to their tertiary structure (tetrameric and monomeric, respectively), we postulated that the multi-valence of jArtinM accounts for its superiority in promoting clustering of cell surface glycoreceptors and activation. The jArtinM and rArtinM activation effect exerted on spleen cells was reproduced on purified CD4(+) T cells. Our results suggest that ArtinM interaction with T cells leads to responses that may act in concert with the interleukin-12 produced by antigen-presenting cells to modulate immunity toward the T helper 1 axis. Further studies are necessary to dissect ArtinM/T-cell interactions to more fully understand the immunomodulation induced by carbohydrate recognition.

  4. The recognition of N-glycans by the lectin ArtinM mediates cell death of a human myeloid leukemia cell line.

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    Fernanda Caroline Carvalho

    Full Text Available ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus (jackfruit, interacts with N-glycosylated receptors on the surface of several cells of hematopoietic origin, triggering cell migration, degranulation, and cytokine release. Because malignant transformation is often associated with altered expression of cell surface glycans, we evaluated the interaction of ArtinM with human myelocytic leukemia cells and investigated cellular responses to lectin binding. The intensity of ArtinM binding varied across 3 leukemia cell lines: NB4>K562>U937. The binding, which was directly related to cell growth suppression, was inhibited in the presence of Manα1-3(Manα1-6Manβ1, and was reverted in underglycosylated NB4 cells. ArtinM interaction with NB4 cells induced cell death (IC(50 = 10 µg/mL, as indicated by cell surface exposure of phosphatidylserine and disruption of mitochondrial membrane potential unassociated with caspase activation or DNA fragmentation. Moreover, ArtinM treatment of NB4 cells strongly induced reactive oxygen species generation and autophagy, as indicated by the detection of acidic vesicular organelles in the treated cells. NB4 cell death was attributed to ArtinM recognition of the trimannosyl core of N-glycans containing a ß1,6-GlcNAc branch linked to α1,6-mannose. This modification correlated with higher levels of N-acetylglucosaminyltransferase V transcripts in NB4 cells than in K562 or U937 cells. Our results provide new insights into the potential of N-glycans containing a β1,6-GlcNAc branch linked to α1,6-mannose as a novel target for anti-leukemia treatment.

  5. Recognition of TLR2 N-glycans: critical role in ArtinM immunomodulatory activity.

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    Vania Sammartino Mariano

    Full Text Available TLR2 plays a critical role in the protection against Paracoccidioides brasiliensis conferred by ArtinM administration. ArtinM, a D-mannose-binding lectin from Artocarpus heterophyllus, induces IL-12 production in macrophages and dendritic cells, which accounts for the T helper1 immunity that results from ArtinM administration. We examined the direct interaction of ArtinM with TLR2using HEK293A cells transfected with TLR2, alone or in combination with TLR1 or TLR6, together with accessory proteins. Stimulation with ArtinM induced NF-κB activation and interleukin (IL-8 production in cells transfected with TLR2, TLR2/1, or TLR2/6. Murine macrophages that were stimulated with ArtinM had augmented TLR2 mRNA expression. Furthermore, pre-incubation of unstimulated macrophages with an anti-TLR2 antibody reduced the cell labeling with ArtinM. In addition, a microplate assay revealed that ArtinM bound to TLR2 molecules that had been captured by specific antibodies from a macrophages lysate. Notably,ArtinM binding to TLR2 was selectively inhibited when the lectin was pre-incubated with mannotriose. The biological relevance of the direct interaction of ArtinM with TLR2 glycans was assessed using macrophages from TLR2-KOmice, which produced significantly lower levels of IL-12 and IL-10 in response to ArtinM than macrophages from wild-type mice. Pre-treatment of murine macrophages with pharmacological inhibitors of signaling molecules demonstrated the involvement of p38 MAPK and JNK in the IL-12 production induced by ArtinM and the involvement ofPI3K in IL-10 production. Thus, ArtinM interacts directly with TLR2 or TLR2 heterodimers in a carbohydrate recognition-dependent manner and functions as a TLR2 agonist with immunomodulatory properties.

  6. Recombinant ArtinM activates mast cells.

    Science.gov (United States)

    Barbosa-Lorenzi, Valéria Cintra; Cecilio, Nerry Tatiana; de Almeida Buranello, Patricia Andressa; Pranchevicius, Maria Cristina; Goldman, Maria Helena S; Pereira-da-Silva, Gabriela; Roque-Barreira, Maria Cristina; Jamur, Maria Célia; Oliver, Constance

    2016-07-04

    Mast cells are hematopoietically derived cells that play a role in inflammatory processes such as allergy, as well as in the immune response against pathogens by the selective and rapid release of preformed and lipid mediators, and the delayed release of cytokines. The native homotetrameric lectin ArtinM, a D-mannose binding lectin purified from Artocarpus heterophyllus seeds, is one of several lectins that are able to activate mast cells. Besides activating mast cells, ArtinM has been shown to affect several biological responses, including immunomodulation and acceleration of wound healing. Because of the potential pharmacological application of ArtinM, a recombinant ArtinM (rArtinM) was produced in Escherichia coli. The current study evaluated the ability of rArtinM to induce mast cell degranulation and activation. The glycan binding specificity of rArtinM was similar to that of jArtinM. rArtinM, via its CRD, was able to degranulate, releasing β-hexosaminidase and TNF-α, and to promote morphological changes on the mast cell surface. Moreover, rArtinM induced the release of the newly-synthesized mediator, IL-4. rArtinM does not have a co-stimulatory effect on the FcεRI degranulation via. The IgE-dependent mast cell activation triggered by rArtinM seems to be dependent on NFkB activation. The lectin rArtinM has the ability to activate and degranulate mast cells via their CRDs. The present study indicates that rArtinM is a suitable substitute for the native form, jArtinM, and that rArtinM may serve as an important and reliable pharmacological agent.

  7. Effect of ArtinM on Human Blood Cells During Infection With Paracoccidioides brasiliensis.

    Science.gov (United States)

    Ruas, Luciana P; Genaro, Livia M; Justo-Junior, Amauri S; Coser, Lilian O; de Castro, Lívia F; Trabasso, Plinio; Mamoni, Ronei L; Roque-Barreira, Maria-Cristina; Blotta, Maria-Heloisa S L

    2018-01-01

    Infections caused by fungi are prominent in our environment and can be potentially fatal. paracoccidioidomycosis (PCM), caused by fungi of the Paracoccidioides genus, is the most frequent systemic mycosis in Brazil and the main cause of death among immunocompetent individuals. The antifungal therapy for PCM is usually effective but side effects and relapses are often reported. The latter could be avoided with alternative or complementary therapies aimed at boosting the immune response to combat this pathogen. Recent reports have pointed at the importance of an effective cellular immune response, with the participation of Th1 cells, in the resistance to and control of Paracoccidioides infection. The ArtinM lectin, extracted from jackfruit ( Artocarpus heterophyllus ) seeds, exhibits immunomodulatory activity against several intracellular pathogens, including Paracoccidioides brasiliensis , by promoting the development of a Th1 immune response. The aim of this work was to characterize the effect of ArtinM on peripheral blood cells of patients with PCM and on those of control individuals infected with fungal yeasts cells in vitro . Our results demonstrate that ArtinM activates human neutrophils in vitro , leading to an increase in cytokine production and CD54 expression. ArtinM activated P. brasiliensis -infected neutrophils from both healthy individuals and patients with PCM. This activation was not dependent on the dectin-1 receptor, because pre-incubation with laminarin, a dectin-1 receptor blocker, did not reverse the activated state of the cells. ArtinM also stimulated human peripheral blood mononuclear cells to secrete pro-inflammatory Th1-related cytokines, which are protective against Paracoccidioides infection. These data support the immunostimulatory action of ArtinM and encourage new studies using the lectin for the immunotherapy of PCM.

  8. Water-borne hyphomycetes in tree canopies of Kaiga (Western Ghats, India

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    Naga M. Sudheep

    2013-12-01

    Full Text Available The canopy samples such as trapped leaf litter, trapped sediment (during summer, stemflow and throughfall (during monsoon from five common riparian tree species (Artocarpus heterophyllus, Cassia fistula, Ficus recemosa, Syzygium caryophyllatum and Xylia xylocarpa in Kaiga forest stand of the Western Ghats of southwest India were evaluated for the occurrence of water-borne hyphomycetes. Partially decomposed trapped leaf litter was incubated in bubble chambers followed by filtration to assess conidial output. Sediments accumulated in tree holes or junction of branches were shaken with sterile leaf disks in distilled water followed by incubation of leaf disks in bubble chamber and filtration to find out colonized fungi. Stemflow and throughfall samples were filtered directly to collect free conidia. From five canopy niches, a total of 29 water-borne hyphomycetes were recovered. The species richness was higher in stemflow and throughfall than trapped leaf litter and sediments (14-16 vs. 6-10 species. Although sediments of Syzygium caryophyllatum were acidic (5.1, the conidial output was higher than other tree species. Stemflow and throughfall of Xylea xylocarpa even though alkaline (8.5-8.7 showed higher species richness (6-12 species as well as conidial load than rest of the tree species. Flagellospora curvula and Triscelophorus acuminatus were common in trapped leaf litter and sediments respectively, while conidia of Anguillospora crassa and A. longissima were frequent in stemflow and throughfall. Diversity of water-borne hyphomycetes was highest in throughfall of Xylea xylocarpa followed by throughfall of Ficus recemosa. Our study reconfirms the occurrence and survival of diverse water-borne hyphomycetes in different niches of riparian tree canopies of the Western Ghats during wet and dry regimes and predicts their possible role in canopy as saprophytes, endophytes and alternation of life cycle between canopy and aquatic habitats.

  9. KARAKTERISTIK FISIKOKIMIA DAN ANTIBAKTERI VIRGIN COCONUT OIL HASIL FERMENTASI BAKTERI ASAM LAKTAT [Physicochemical and Antibacterial Characteristics of Virgin Coconut Oil Fermented with Lactic Acid Bacteria

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    Anton Rahmadi*

    2013-12-01

    Full Text Available Destabilization of oil-water emulsion in coconut milk, in the production of virgin coconut oil (VCO, can be accelerated with the utilization of lactic acid bacteria fermentation. This research was aimed to determine physicochemical and antibacterial characteristics of VCO from coconut hybrid variety fermented with L. casei of Yakult® and two isolates of L. plantarum from mandai (traditionally fermented Artocarpus campeden and coconut water. The observed physicochemical of VCO included yield, specific gravity, moisture content, saponification value, peroxide value, and free fatty acid. The antibacterial activity was subjected to the well diffusion method against E. coli and S. aureus with chloramphenicol as the positive control. L. casei yielded the best VCO-BAL at 34.5% (v/v, while L. plantarum from mandai and coconut water yielded 29.5% (v/v and 25.3% (v/v, respectively. VCO-BAL from L. casei had the lightest specific gravity of 0.84±0.04 g.mL-1. Average of measured moisture contents (0.03-0.05%, saponification values (161.3-163.6, peroxide values (0.53-0.86, and free fatty acids (0.11-0.12% of the three VCO-BALs were not significantly (p>0.05 different with respect to control. VCO-BAL produced from L. plantarum of coconut water did not exhibit better antibacterial activity compared to control. VCO-BAL from L. casei demonstrated highest antibacterial activity against E. coli, 6.45±0.50 mm (58.1% of positive control and S. aureus, 5.23±0.40 mm (51.3% of positive control. It is deduced that antibacterial activity from VCO-BAL is contributed by hydrophobic bacteriocins.

  10. Smile, you are on camera or in a live trap! The role of mammals in dispersion of jackfruit and native seeds in Ilha Grande State Park, Brazil

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    Daniel Santana Lorenzo Raíces

    2017-11-01

    Full Text Available The alien species Artocarpus heterophyllus, originally from India, was introduced to Brazil in the colonial period and has become invasive in some areas in the Atlantic Forest. Its fruits can weigh 35 kg and produce up to 500 seeds each. In their native range they are dispersed by turtles, rodents, monkeys, wild pigs and elephants. This study aimed to investigate the influence of mammals in jackfruit predation and seed dispersal, as well as the influence of jackfruit in native plant species dispersal by mammals, in the Ilha Grande State Park, Southeastern Brazil. Seeds with and without mesocarp were tied to thread spools and checked for predation and dispersion on 37 sites. We recorded mesocarp or jackfruit seed feeding on video. Six species of mammals were recorded feeding on jackfruit, but Trinomys dimidiatus, Didelphis aurita and Cuniculus paca accounted for 92% of all records. Cuniculus paca and Trinomys dimidiatus preyed and dispersed seeds while Didelphis aurita consumed mesocarp only. Seeds with mesocarp were more preyed on than seeds without mesocarp and its consumption was lower during more intense fruit production. Hence, jackfruit production can exceed the capacity of mammals to consume its seeds in areas where jackfruit density is high. Faeces of small mammals were collected in areas with (10 grids and without jackfruits (8 grids and analysed for the presence of native seeds. Twelve small mammal species were captured in areas with and without jackfruits, but faeces of 11 species were collected. Didelphis aurita dispersed proportionally more native seeds in area without jackfruits. Our results showed that mammals are playing a negative role helping to disperse jackfruit trees, and this is occurring in different ways depending on mammal species.

  11. Dissemination of Aleurocanthus woglumi in citrus plants, its natural enemies and new host plants in the state of Rio de Janeiro, Brazil

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    Rodrigo Garcia Alvim

    Full Text Available ABSTRACT: Aleurocanthus woglumi is an exotic pest, widely disseminated in Brazil, with a high preference for citrus plants, but with a polyphagous feeding habit. The contribution of the state of Rio de Janeiro in the Brazilian production of citrus fruits is small; however, there are still public policies that encourage citrus production in the state. In 2010, the appearance of this pest in Rio de Janeiro was confirmed in the municipality of Cachoeiras de Macacu. The objectives of this research were to evaluate the dissemination of A. woglumi in this state, conduct a survey of new species of host plants, identify and evaluate the population of natural enemies present at two orchards that grow 'Tahiti' limes ( Citrus latifolia infested by the pest in Cachoeiras de Macacu, RJ, Brazil. In 19 municipalities, leaves of citrus and other species of plants presenting aleyrodid nymphs were collected, and yellow sticky traps were installed to capture adults for subsequent identification. At the 'Tahiti' lime orchards, the leaf collections were done to confirm the species of Aleyrodidae , and regarding A. woglumi , the natural enemies associated with this pest were collected directly from the infested plants. The results showed that A. woglumi is wide-spread in 12 municipalities. Three new host plants for A. woglumi were identified: Artocarpus heterophyllus ( Moraceae , Pouteria caimito ( Sapotaceae and Struthanthus flexicaulis ( Loranthaceae . In Cachoeiras de Macacu, a new species of parasitoid of A. woglumi nymphs was named: Encarsia pergandiella (Hymenoptera: Aphelinidae , while the insects in the Coccinellidae (Coleoptera family have stood out as predators of this pest.

  12. Fraction A of armadillo submandibular glycoprotein and its desialylated product as sialyl-Tn and Tn receptors for lectins.

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    Wu, A M; Shen, F; Herp, A; Song, S C; Wu, J H

    1995-02-27

    Fraction A of the armadillo submandibular glycoprotein (ASG-A) is one of the simplest glycoproteins among mammalian salivary mucins. The carbohydrate side chains of this mucous glycoprotein have one-third of the NeuAc alpha 2-->6GalNAc (sialyl-Tn) sequence and two thirds of Tn (GalNAc alpha-->Ser/Thr) residues. Those of the desialylated product (ASG-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinant). When the binding properties of these glycoproteins were tested by a precipitin assay with Gal, GalNAc and GlcNAc specific lectins, it was found that ASG-Tn reacted strongly with all of the Tn-active lectins and completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPA), and Artocarpus integrifolia (jacalin) lectins. However, it precipitated poorly or negligibly with Ricinus communis (RCA1); Dolichos biflorus (DBA); Viscum album, ML-I; Arachis hypogaea (PNA), and Triticum vulgaris (WGA). The reactivity of ASG-A (sialyl-Tn) was as active as that of ASG-Tn with MPA and less or slightly less active than that of ASG-Tn with VVL-A+B, VVL-B4, HPA, WFA, and jacalin, as one-third of its Tn was sialylated. These findings indicate that ASG-A and its desialylated product (ASG-Tn) are highly useful reagents for the differentiation of Tn, T (Gal beta 1-->3GalNAc), A (GalNAc alpha 1-->3Gal) or Gal specific lectins and monoclonal antibodies against such epitopes.

  13. Interaction of hamster submaxillary sialyl-Tn and Tn glycoproteins with Gal, GalNAc and GlcNAc specific lectins.

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    Wu, A M; Shen, F; Herp, A; Wu, J H

    1994-04-01

    Hamster submaxillary glycoprotein (HSM), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. The Thr and Ser residues in the protein core account for more than half of all of the amino acid residues, while Lys, Glu, Pro and Ala are the major components of the remaining portion of amino acids. The carbohydrate side chains of this mucous glycoprotein have mainly the NeuAc-GalNAc-(sialyl-Tn) sequence (HSM), and those of the desialylated product (HSM-Tn) are almost exclusively unsubstituted GalNAc residues (Tn determinants). The binding properties of sialyl-Tn (HSM) and asialo-HSM (HSM-Tn) glycoproteins were tested by precipitin assay with Gal, GalNAc and GlcNAc specific lectins. The HSM-Tn completely precipitated Vicia villosa (VVL both B4 and mixture of A and B), Maclura pomifera (MPL), and Artocarpus integrifolia (Jacalin) lectins; less than 2 micrograms of HSM-Tn were required for precipitating 50% of 5.0-6.3 micrograms lectin nitrogen added. HSM-Tn also reacted well with Helix pomatia lectin (HPL), Wistaria floribunda lectin (WFL) and Abrus precatorius agglutinin (APA) and precipitated in each case over 81% of the lectin nitrogen added. The reactivity of HSM-Tn with other lectins (Ricinus communis, RCA1; Dolichol biflorus, DBL; Viscum album, ML-I; Arachis hypogaea, PNA, and Triticum vulgaris, WGA) was weak or negligible. The activity of sialyl-Tn (HSM) was more restricted; HSM reacted well with Jacalin, moderately with MPL and VVL-B4, but was inactive or only weakly with the other lectins used. These findings indicate that HSM and its desialylated product (HSM-Tn) are highly useful reagents for the differentiation of Tn and T/Gal specific lectins and for anti-T, Tn and Af monoclonal antibodies.

  14. Raiders of the lost bark: orangutan foraging strategies in a degraded landscape.

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    Gail Campbell-Smith

    Full Text Available Deforestation is rapidly transforming primary forests across the tropics into human-dominated landscapes. Consequently, conservationists need to understand how different taxa respond and adapt to these changes in order to develop appropriate management strategies. Our two year study seeks to determine how wild Sumatran orangutans (Pongo abelii adapt to living in an isolated agroforest landscape by investigating the sex of crop-raiders related to population demographics, and their temporal variations in feeding behaviour and dietary composition. From focal animal sampling we found that nine identified females raided cultivated fruits more than the four males. Seasonal adaptations were shown through orangutan feeding habits that shifted from being predominantly fruit-based (56% of the total feeding time, then 22% on bark to the fallback food of bark (44%, then 35% on fruits, when key cultivated resources such as jackfruit (Artocarpus integer, were unavailable. Cultivated fruits were mostly consumed in the afternoon and evening, when farmers had returned home. The finding that females take greater crop-raiding risks than males differs from previous human-primate conflict studies, probably because of the low risks associated (as farmers rarely retaliated and low intraspecific competition between males. Thus, the behavioral ecology of orangutans living in this human-dominated landscape differs markedly from that in primary forest, where orangutans have a strictly wild food diet, even where primary rainforests directly borders farmland. The importance of wild food availability was clearly illustrated in this study with 21% of the total orangutan feeding time being allocated to feeding on cultivated fruits. As forests are increasingly converted to cultivation, humans and orangutans are predicted to come into conflict more frequently. This study reveals orangutan adaptations for coexisting with humans, e.g. changes in temporal foraging patterns, which

  15. Effects of plant lectins on in vitro fibroblast proliferation

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    Ana Maria Sell

    2003-06-01

    Full Text Available Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.O objetivo deste trabalho foi avaliar a influência das lectinas PHA, WGA, jacalina e artocarpina sobre a proliferação de fibroblastos in vitro. Para tanto, fibroblastos gengivais de voluntários saudáveis foram cultivados, por cinco dias, em DMEM suplementado com soro bovino fetal (10% v/v e na presença das lectinas nas concentrações finais de 0.1 a 300 µg/mL. A adesão, o crescimento e a morfologia celular foram acompanhados por microscopia de inversão e contraste de fase. O índice de proliferação foi avaliado pelo método calorimétrico usando MTT. As lectinas não alteraram a adesão inicial dos fibroblastos à placa de poliestireno. PHA e WGA, nas concentrações de 10 a 20 µg/mL, diminuíram significativamente a proliferação celular. Nestas concentrações a morfologia celular é alterada e acima de 80 µg/mL, houve100% de morte celular. As lectinas jacalina e artocarpina não influenciaram a proliferação celular.

  16. Functional nonredundancy of elephants in a disturbed tropical forest.

    Science.gov (United States)

    Sekar, Nitin; Lee, Chia-Lo; Sukumar, Raman

    2017-10-01

    Conservation efforts are often motivated by the threat of global extinction. Yet if conservationists had more information suggesting that extirpation of individual species could lead to undesirable ecological effects, they might more frequently attempt to protect or restore such species across their ranges even if they were not globally endangered. Scientists have seldom measured or quantitatively predicted the functional consequences of species loss, even for large, extinction-prone species that theory suggests should be functionally unique. We measured the contribution of Asian elephants (Elephas maximus) to the dispersal of 3 large-fruited species in a disturbed tropical moist forest and predicted the extent to which alternative dispersers could compensate for elephants in their absence. We created an empirical probability model with data on frugivory and seed dispersal from Buxa Tiger Reserve, India. These data were used to estimate the proportion of seeds consumed by elephants and other frugivores that survive handling and density-dependent processes (Janzen-Connell effects and conspecific intradung competition) and germinate. Without compensation, the number of seeds dispersed and surviving density-dependent effects decreased 26% (Artocarpus chaplasha), 42% (Careya arborea), and 72% (Dillenia indica) when elephants were absent from the ecosystem. Compensatory fruit removal by other animals substantially ameliorated these losses. For instance, reductions in successful dispersal of D. indica were as low as 23% when gaur (Bos gaurus) persisted, but median dispersal distance still declined from 30% (C. arborea) to 90% (A. chaplasha) without elephants. Our results support the theory that the largest animal species in an ecosystem have nonredundant ecological functionality and that their extirpation is likely to lead to the deterioration of ecosystem processes such as seed dispersal. This effect is likely accentuated by the overall defaunation of many tropical

  17. Radiation doses for Marshall Islands Atolls Affected by U.S. Nuclear Testing:All Exposure Pathways, Remedial Measures, and Environmental Loss of 137Cs

    Energy Technology Data Exchange (ETDEWEB)

    Robison, W L; Hamilton, T F

    2009-04-20

    Rongelap Atoll (Robison et al., 1994; Simon et al., 1997), and Utrok Island at Utrok Atoll (Robison, et al., 1999) indicate that about 95-99% of the total estimated dose to people who may return to live at the atolls today (Utrok Island is populated) is the result of exposure to {sup 137}Cs. External gamma exposure from {sup 137}Cs in the soil accounts for about 10 to 15% of the total dose and {sup 137}Cs ingested during consumption of local food crops such as drinking coconut meat and fluid (Cocos nucifera L.), copra meat and milk, Pandanus fruit, and breadfruit accounts for about 85 to 90%. The other 1 to 2% of the estimated dose is from {sup 90}Sr, {sup 239+240}Pu, and {sup 241}Am. The {sup 90}Sr exposure is primarily through the food chain while the exposure to {sup 239+240}Pu, and {sup 241}Am is primarily via the inhalation pathway as a result of breathing re-suspended soil particles.

  18. Systemic effects in naïve mice injected with immunomodulatory lectin ArtinM.

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    Patrícia Kellen Martins Oliveira Brito

    Full Text Available Toll-like receptors (TLR contain N-glycans, which are important glycotargets for plant lectins, to induce immunomodulation. The lectin ArtinM obtained from Artocarpus heterophyllus interacts with TLR2 N-glycans to stimulate IL-12 production by antigen-presenting cells and to drive the immune response toward the Th1 axis, conferring resistance against intracellular pathogens. This immunomodulatory effect was demonstrated by subcutaneously injecting (s.c. ArtinM (0.5 μg in infected mice. In this study, we evaluated the systemic implications of ArtinM administration in naïve BALB/c mice. The mice were s.c. injected twice (7 days interval with ArtinM (0.5, 1.0, 2.5, or 5.0 μg, LPS (positive control, or PBS (negative control and euthanized after three days. None of the ArtinM-injected mice exhibited change in body weight, whereas the relative mass of the heart and lungs diminished in mice injected with the highest ArtinM dose (5.0 μg. Few and discrete inflammatory foci were detected in the heart, lung, and liver of mice receiving ArtinM at doses ≥2.5 μg. Moreover, the highest dose of ArtinM was associated with increased serum levels of creatine kinase MB isoenzyme (CK-MB and globulins as well as an augmented presence of neutrophils in the heart and lung. IL-12, IFN-γ, TNF-α, and IL-10 measurements in the liver, kidney, spleen, heart, and lung homogenates revealed decreased IL-10 level in the heart and lung of mice injected with 5.0 μg ArtinM. We also found an augmented frequency of T helper and B cells in the spleen of all ArtinM-injected naïve mice, whereas the relative expressions of T-bet, GATA-3, and ROR-γt were similar to those in PBS-injected animals. Our study demonstrates that s.c. injection of high doses of ArtinM in naïve mice promotes mild inflammatory lesions and that a low immunomodulatory dose is innocuous to naïve mice.

  19. Systemic effects in naïve mice injected with immunomodulatory lectin ArtinM

    Science.gov (United States)

    Oliveira Brito, Patrícia Kellen Martins; Gonçalves, Thiago Eleutério; Fernandes, Fabrício Freitas; Miguel, Camila Botelho; Rodrigues, Wellington Francisco; Lazo Chica, Javier Emílio; Roque-Barreira, Maria Cristina

    2017-01-01

    Toll-like receptors (TLR) contain N-glycans, which are important glycotargets for plant lectins, to induce immunomodulation. The lectin ArtinM obtained from Artocarpus heterophyllus interacts with TLR2 N-glycans to stimulate IL-12 production by antigen-presenting cells and to drive the immune response toward the Th1 axis, conferring resistance against intracellular pathogens. This immunomodulatory effect was demonstrated by subcutaneously injecting (s.c.) ArtinM (0.5 μg) in infected mice. In this study, we evaluated the systemic implications of ArtinM administration in naïve BALB/c mice. The mice were s.c. injected twice (7 days interval) with ArtinM (0.5, 1.0, 2.5, or 5.0 μg), LPS (positive control), or PBS (negative control) and euthanized after three days. None of the ArtinM-injected mice exhibited change in body weight, whereas the relative mass of the heart and lungs diminished in mice injected with the highest ArtinM dose (5.0 μg). Few and discrete inflammatory foci were detected in the heart, lung, and liver of mice receiving ArtinM at doses ≥2.5 μg. Moreover, the highest dose of ArtinM was associated with increased serum levels of creatine kinase MB isoenzyme (CK-MB) and globulins as well as an augmented presence of neutrophils in the heart and lung. IL-12, IFN-γ, TNF-α, and IL-10 measurements in the liver, kidney, spleen, heart, and lung homogenates revealed decreased IL-10 level in the heart and lung of mice injected with 5.0 μg ArtinM. We also found an augmented frequency of T helper and B cells in the spleen of all ArtinM-injected naïve mice, whereas the relative expressions of T-bet, GATA-3, and ROR-γt were similar to those in PBS-injected animals. Our study demonstrates that s.c. injection of high doses of ArtinM in naïve mice promotes mild inflammatory lesions and that a low immunomodulatory dose is innocuous to naïve mice. PMID:29084277

  20. Assessment and Comparison of salt Content in Mangrove Plants in Sri Lanka

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    N. P. Dissanayake

    2009-09-01

    Full Text Available Due to the predicted threats of global warming and sea level rise, the salt tolerance and salt accumulative abilities of plants have become popular contentious topics. Mangroves are one of the major groups of salt tolerant plants and several mechanisms are known as instrumental in their salt tolerance. Salt excretion through leaf drop is given as one, but its validity is questioned by some recent works compelling the necessity for further studies. Knowledge of the salt contents in different mangrove plants is a pre requisite for such studies. Hence, this study aimed to quantify and compare the salt content in mature leaves of nine mangrove species in Sri Lanka., i.e. Aegiceras corniculatum, Avicennia marina, Avicennia officinalis, Bruguiera gymnorrhiza, Bruguiera sexangula, Ceriops tagal, Excoecaria agallocha, Lumnitzera racemosa, Rhizophora apiculata and Rhizophora mucronata which are growing in the same mangrove system; the Rekawa lagoon in Sri Lanka. Two species of non mangrove plants, Gliricidia sepium and Artocarpus heterophyllus, which were growing in inland areas were also selected for comparison. The concentration of Na+ in leaves was considered as a measure of the salt concentration. The Na+ in leaves was extracted by acid digestion and quantified by flame photometry. The salt content of mangroves was measured under two contrasting hydrological situations: at the highest and lowest water levels of the lagoon. Rekawa lagoon can be considered as a ‘barrier built estuary’, the highest water level occurs when the lagoon mouth is blocked due to the formation of the sand bar and the water level is increased by fresh water inflow, inundating the total mangrove area and decreasing the soil/water salinity. The water level of the lagoon becomes lowest when the lagoon mouth is opened (naturally or by dredging and lagoon water is flushed out to the sea. Then the salinity of lagoon water becomes high due to sea water influx. The results showed

  1. Eucalyptus water use greater than rainfall input - possible explanation from southern India

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    I. R. Calder

    1997-01-01

    Full Text Available Hydrological and silvicultural studies carried out in southern India on the effects of plantations of Eucalyptus and other fast growing exotic tree species have determined the impacts of these plantations on water resources, erosion, soil nutrient status and growth rates at sites of differing rainfall and soil depth in Karnataka. Whilst providing new information on these issues, the studies also raised two important questions: what was the explanation for the anomalous result that the water use of 3400 mm from Eucalyptus plantations at Hosakote over a three year period exceeded the rainfall of 2100 mm over the same period and why were growth rates of woodlots on most farmer's fields higher than those of plantations on land owned by the Karnataka Forest Department? The records of the soil moisture depletion patterns under these plantations from the day of planting provide the basis for the answers to both questions: i whilst roots are penetrating into deeper soil layers, they are able to extract from a reservoir of water additional to that available from the rainfall each year, ii farmer's land on which short rooted agricultural crops have been grown previously is likely to have a much higher soil water status than land previously under forest or scrub vegetation. These new studies have also established that the development of the drying front under the Eucalyptus camaldulensis plantations is very rapid, indicating average root extension rates in excess of 2.5 m per year, whilst those under Tectona grandis and Artocarpus heterophyllus advanced at approximately half the rate. These results have obvious implications for the long term sustainability of growth rates from these plantations and the recharge of groundwater. The authors believe that this study may be the first to report neutron probe soil moisture depletion observations, from the date of planting, beneath tree plantations in a dry climate. The extent to which the roots were able to

  2. Eucalyptus water use greater than rainfall input - possible explanation from southern India

    Science.gov (United States)

    Calder, I. R.; Rosier, P. T. W.; Prasanna, K. T.; Parameswarappa, S.

    Hydrological and silvicultural studies carried out in southern India on the effects of plantations of Eucalyptus and other fast growing exotic tree species have determined the impacts of these plantations on water resources, erosion, soil nutrient status and growth rates at sites of differing rainfall and soil depth in Karnataka. Whilst providing new information on these issues, the studies also raised two important questions: what was the explanation for the anomalous result that the water use of 3400 mm from Eucalyptus plantations at Hosakote over a three year period exceeded the rainfall of 2100 mm over the same period and why were growth rates of woodlots on most farmer's fields higher than those of plantations on land owned by the Karnataka Forest Department? The records of the soil moisture depletion patterns under these plantations from the day of planting provide the basis for the answers to both questions: i) whilst roots are penetrating into deeper soil layers, they are able to extract from a reservoir of water additional to that available from the rainfall each year, ii) farmer's land on which short rooted agricultural crops have been grown previously is likely to have a much higher soil water status than land previously under forest or scrub vegetation. These new studies have also established that the development of the drying front under the Eucalyptus camaldulensis plantations is very rapid, indicating average root extension rates in excess of 2.5 m per year, whilst those under Tectona grandis and Artocarpus heterophyllus advanced at approximately half the rate. These results have obvious implications for the long term sustainability of growth rates from these plantations and the recharge of groundwater. The authors believe that this study may be the first to report neutron probe soil moisture depletion observations, from the date of planting, beneath tree plantations in a dry climate. The extent to which the roots were able to penetrate raises the

  3. Pollination implications of the diverse diet of tropical nectar-feeding bats roosting in an urban cave.

    Science.gov (United States)

    Lim, Voon-Ching; Ramli, Rosli; Bhassu, Subha; Wilson, John-James

    2018-01-01

    Intense landscaping often alters the plant composition in urban areas. Knowing which plant species that pollinators are visiting in urban areas is necessary for understanding how landscaping impacts biodiversity and associated ecosystem services. The cave nectar bat, Eonycteris spelaea , is an important pollinator for many plants and is often recorded in human-dominated habitats. Previous studies of the diet of E. spelaea relied on morphological identification of pollen grains found in faeces and on the body of bats and by necessity disregarded other forms of digested plant material present in the faeces (i.e., plant juice and remnants). The main objective of this study was to examine the diet of the nectarivorous bat, E. spelaea, roosting in an urban cave at Batu Caves, Peninsular Malaysia by identifying the plant material present in the faeces of bats using DNA metabarcoding. Faeces were collected under the roost of E. spelaea once a week from December 2015 to March 2016. Plant DNA was extracted from the faeces, Polymerase chain reaction (PCR) amplified at ITS2 and rbcL regions and mass sequenced. The resultant plant operational taxonomic units were searched against NCBI GenBank for identification. A total of 55 species of plants were detected from faeces of E. spelaea including Artocarpus heterophyllus, Duabanga grandiflora and Musa spp. which are likely to be important food resources for the cave nectar bat. Many native plant species that had not been reported in previous dietary studies of E. spelaea were detected in this study including Bauhinia strychnoidea and Urophyllum leucophlaeum , suggesting that E. spelaea remains a crucial pollinator for these plants even in highly disturbed habitats. The detection of many introduced plant species in the bat faeces indicates that E. spelaea are exploiting them, particularly Xanthostemon chrysanthus, as food resources in urban area. Commercial food crops were detected from all of the faecal samples, suggesting that E

  4. Pollination implications of the diverse diet of tropical nectar-feeding bats roosting in an urban cave

    Directory of Open Access Journals (Sweden)

    Voon-Ching Lim

    2018-03-01

    Full Text Available Background Intense landscaping often alters the plant composition in urban areas. Knowing which plant species that pollinators are visiting in urban areas is necessary for understanding how landscaping impacts biodiversity and associated ecosystem services. The cave nectar bat, Eonycteris spelaea, is an important pollinator for many plants and is often recorded in human-dominated habitats. Previous studies of the diet of E. spelaea relied on morphological identification of pollen grains found in faeces and on the body of bats and by necessity disregarded other forms of digested plant material present in the faeces (i.e., plant juice and remnants. The main objective of this study was to examine the diet of the nectarivorous bat, E. spelaea, roosting in an urban cave at Batu Caves, Peninsular Malaysia by identifying the plant material present in the faeces of bats using DNA metabarcoding. Methods Faeces were collected under the roost of E. spelaea once a week from December 2015 to March 2016. Plant DNA was extracted from the faeces, Polymerase chain reaction (PCR amplified at ITS2 and rbcL regions and mass sequenced. The resultant plant operational taxonomic units were searched against NCBI GenBank for identification. Results A total of 55 species of plants were detected from faeces of E. spelaea including Artocarpus heterophyllus, Duabanga grandiflora and Musa spp. which are likely to be important food resources for the cave nectar bat. Discussion Many native plant species that had not been reported in previous dietary studies of E. spelaea were detected in this study including Bauhinia strychnoidea and Urophyllum leucophlaeum, suggesting that E. spelaea remains a crucial pollinator for these plants even in highly disturbed habitats. The detection of many introduced plant species in the bat faeces indicates that E. spelaea are exploiting them, particularly Xanthostemon chrysanthus, as food resources in urban area. Commercial food crops were

  5. Expression of binding properties of Gal/GalNAc reactive lectins by mammalian glycotopes (an updated report).

    Science.gov (United States)

    Wu, A M

    2001-01-01

    Expression of the binding properties of Gal/GalNAc specific lectins, based on the affinity of decreasing order of mammalian glycotopes (determinants) rather than monosaccharide inhibition pattern, is probably one of the best ways to express carbohydrate specifity and should facilitate the selection of lectins as structural probes for studying mammalian glycobiology. Eleven mammalian structural units have been selected to express the binding domain of applied lectins. They are: 1. F, GalNAcalpha1 --> 3GalNAc; 2. A, GalNAcalpha1 --> 3Gal; 3. T, Galbeta1 --> 3GalNAc; 4. I, Galbeta 1 --> 3GlcNAc; 5. II, Galbeta1 --> 4GlcNAc; 6. B, Galalpha1 --> 3Gal; 7. E, Galalpha1--> 4Gal; 8. L, Galbeta1 --> 4Glc; 9. P, GalNAcbeta1 --> 3Gal; 10. S, GalNAcbeta1 --> 4Gal and 11. Tn, GalNAcalpha1 --> 4Ser (Thr) of the peptide chain. Thus, the carbohydrate specificity of Gal/GalNAc reactive lectins can be divided into classes according to their highest affinity for the above disaccharides and/or Tn residue. Examples of the binding properties of these lectins can be demonstrated by Ricimus communis agglutinin (RCA1), grouped as II specific lectin and its binding property is II > I > B > T; Ahrus precatorius agglutinin (APA), classified as T and its carbohydrate specificity is T > I/II > E > B > Tn; Artocarpus integrifolia (jacalin, AIL), as T/Tn specific and its binding reactivity is T > Tn > I (II) and Geodia cydonium (GCL), as F/A specific, and with affinity for F > Ah [GalNAcalpha1-->43(L(Fuc)alpha1-->2)Gal] > I > L. Due to the multiple reactivity of lectins toward mammalian glycotopes, the possible existence of different combining sites or subsites in the same molecule has to be examined, and the differential binding properties of these combining sites (if any) have to be characterized. To establish the relationship among the amino acid sequences of the combining sites of plant lectins and mammalian glycotopes should be an important direction to be addressed in lectinology.

  6. Characterization and optimization of ArtinM lectin expression in Escherichia coli

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    Pranchevicius Maria-Cristina S

    2012-08-01

    Full Text Available Abstract Background ArtinM is a d-mannose-specific lectin from Artocarpus integrifolia seeds that induces neutrophil migration and activation, degranulation of mast cells, acceleration of wound healing, induction of interleukin-12 production by macrophages and dendritic cells, and protective T helper 1 immune response against Leishmania major, Leishmania amazonensis and Paracoccidioides brasiliensis infections. Considering the important biological properties of ArtinM and its therapeutic applicability, this study was designed to produce high-level expression of active recombinant ArtinM (rArtinM in Escherichia coli system. Results The ArtinM coding region was inserted in pET29a(+ vector and expressed in E. coli BL21(DE3-Codon Plus-RP. The conditions for overexpression of soluble ArtinM were optimized testing different parameters: temperatures (20, 25, 30 or 37°C and shaking speeds (130, 200 or 220 rpm during induction, concentrations of the induction agent IPTG (0.01-4 mM and periods of induction (1-19 h. BL21-CodonPlus(DE3-RP cells induced under the optimized conditions (incubation at 20°C, at a shaking speed of 130 rpm, induction with 0.4 mM IPTG for 19 h resulted in the accumulation of large amounts of soluble rArtinM. The culture provided 22.4 mg/L of rArtinM, which activity was determined by its one-step purification through affinity chromatography on immobilized d-mannose and glycoarray analysis. Gel filtration showed that rArtinM is monomeric, contrasting with the tetrameric form of the plant native protein (jArtinM. The analysis of intact rArtinM by mass spectrometry revealed a 16,099.5 Da molecular mass, and the peptide mass fingerprint and esi-cid-ms/ms of amino acid sequences of peptides from a tryptic digest covered 41% of the total ArtinM amino acid sequence. In addition, circular dichroism and fluorescence spectroscopy of rArtinM indicated that its global fold comprises β-sheet structure. Conclusions Overall, the

  7. Root strength of tropical plants - An investigation in the Western Ghats of Kerala, India

    Science.gov (United States)

    Lukose Kuriakose, S.; van Beek, L. P. H.; van Westen, C. J.

    2009-04-01

    Earlier research on debris flows in the Tikovil River basin of the Western Ghats concluded that root cohesion is significant in maintaining the overall stability of the region. In this paper we present the most recent results (December 2008) of root tensile strength tests conducted on nine species of plants that are commonly found in the region. They are 1) Rubber (Hevea Brasiliensis), 2) Coconut Palm (Cocos nucifera), 3) Jackfruit trees (Artocarpus heterophyllus), 4) Teak (Tectona grandis), 5) Mango trees (Mangifera indica), 6) Lemon grass (Cymbopogon citratus), 7) A variety of Tamarind (Garcinia gummigutta), 8) Coffee (Coffea Arabica) and Tea (Camellia sinensis). About 1500 samples were collected of which only 380 could be tested (in the laboratory) due to breakage of roots during the tests. In the successful tests roots failed in tension. Roots having diameters between 2 mm and 12 mm were tested. Each sample tested has a length of 15 cm. Results indicate that the roots of Coffee, Tamarind, Lemon grass and Jackfruit are the strongest of the nine plant types tested whereas Tea and Teak plants had the most fragile roots. Coconut roots behaved atypical to the others, as the bark of the roots was crushed and slipped from the clamp when tested whereas its internal fiber was the strongest of all tested. Root tensile strength decreases with increasing diameters, Rubber showing more ductile behaviour than Coffee and Tamarind that behaved more brittle, root tensile strength increasing exponentially for finer roots. Teak and Tea showed almost a constant root tensile strength over the range of diameters tested and little variability. Jack fruit and mango trees showed the largest variability, which may be explained by the presence of root nodules, preventing the derivation of an unequivocal relationship between root diameters and tensile strength. This results in uncertainty of root strength estimates that are applicable. These results provide important information to

  8. Artonin E induces p53-independent G1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cells

    Directory of Open Access Journals (Sweden)

    Etti IC

    2017-03-01

    Full Text Available Imaobong Christopher Etti,1,2 Abdullah Rasedee,3 Najihah Mohd Hashim,4 Ahmad Bustamam Abdul,5 Arifah Kadir,6 Swee Keong Yeap,7 Peter Waziri,5 Ibrahim Malami,5 Kian Lam Lim,8 Christopher J Etti9 1Pharmacology and Toxicology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia; 2Department of Pharmacology and Toxicology, University of Uyo, Uyo, Nigeria; 3Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia; 4Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; 5MAKNA-Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia; 6Department of Veterinary Preclinical Science, Universiti Putra Malaysia, Serdang, Malaysia; 7Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia; 8Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Cheras, Selangor, Malaysia; 9Department of Agricultural and Food Engineering, University of Uyo, Uyo, Nigeria Abstract: Artonin E is a prenylated flavonoid compound isolated from the stem bark of Artocarpus elasticus. This phytochemical has been previously reported to be drug-like with full compliance to Lipinski’s rule of five and good physicochemical properties when compared with 95% of orally available drugs. It has also been shown to possess unique medicinal properties that can be utilized in view of alleviating most human disease conditions. In this study, we investigated the cytotoxic mechanism of Artonin E in MCF-7 breast cancer cells, which has so far not been reported. In this context, Artonin E significantly suppressed the breast cancer cell’s viability while inducing apoptosis in a dose-dependent manner. This apoptosis induction was caspase dependent, and it is mediated mainly through the intrinsic pathway with the elevation of total reactive oxygen species

  9. Root reinforcement and its contribution to slope stability in the Western Ghats of Kerala, India

    Science.gov (United States)

    Lukose Kuriakose, Sekhar; van Beek, L. P. H.

    2010-05-01

    The Western Ghats of Kerala, India is prone to shallow landslides and consequent debris flows. An earlier study (Kuriakose et al., DOI:10.1002/esp.1794) with limited data had already demonstrated the possible effects of vegetation on slope hydrology and stability. Spatially distributed root cohesion is one of the most important data necessary to assess the effects of anthropogenic disturbances on the probability of shallow landslide initiation, results of which are reported in sessions GM6.1 and HS13.13/NH3.16. Thus it is necessary to the know the upper limits of reinforcement that the roots are able to provide and its spatial and vertical distribution in such an anthropogenically intervened terrain. Root tensile strength and root pull out tests were conducted on nine species of plants that are commonly found in the region. They are 1) Rubber (Hevea Brasiliensis), 2) Coconut Palm (Cocos nucifera), 3) Jackfruit trees (Artocarpus heterophyllus), 4) Teak (Tectona grandis), 5) Mango trees (Mangifera indica), 6) Lemon grass (Cymbopogon citratus), 7) Gambooge (Garcinia gummi-gutta), 8) Coffee (Coffea Arabica) and 9) Tea (Camellia sinensis). About 1500 samples were collected of which only 380 could be tested (in the laboratory) due to breakage of roots during the tests. In the successful tests roots failed in tension. Roots having diameters between 2 mm and 12 mm were tested. Each sample tested had a length of 15 cm. Root pull out tests were conducted in the field. Root tensile strength vs root diameter, root pull out strength vs diameter, root diameter vs root depth and root count vs root depth relationships were derived. Root cohesion was computed for nine most dominant plants in the region using the perpendicular root model of Wu et al. (1979) modified by Schimidt et al. (2001). A soil depth map was derived using regression kriging as suggested by Kuriakose et al., (doi:10.1016/j.catena.2009.05.005) and used along with the land use map of 2008 to distribute the

  10. IL-17 Induction by ArtinM is Due to Stimulation of IL-23 and IL-1 Release and/or Interaction with CD3 in CD4+ T Cells.

    Science.gov (United States)

    da Silva, Thiago Aparecido; Mariano, Vania Sammartino; Sardinha-Silva, Aline; de Souza, Maria Aparecida; Mineo, Tiago Wilson Patriarca; Roque-Barreira, Maria Cristina

    2016-01-01

    ArtinM is a D-mannose-binding lectin extracted from the seeds of Artocarpus heterophyllus that interacts with TLR2 N-glycans and activates antigen-presenting cells (APCs), as manifested by IL-12 production. In vivo ArtinM administration induces Th1 immunity and confers protection against infection with several intracellular pathogens. In the murine model of Candida albicans infection, it was verified that, in addition to Th1, ArtinM induces Th17 immunity manifested by high IL-17 levels in the treated animals. Herein, we investigated the mechanisms accounting for the ArtinM-induced IL-17 production. We found that ArtinM stimulates the IL-17 production by spleen cells in BALB/c or C57BL/6 mice, a response that was significantly reduced in the absence of IL-23, MyD88, or IL-1R. Furthermore, we showed that ArtinM directly induced the IL-23 mRNA expression and the IL-1 production by macrophages. Consistently, in cell suspensions depleted of macrophages, the IL-17 production stimulated by ArtinM was reduced by 53% and the exogenous IL-23 acted synergistically with ArtinM in promoting IL-17 production by spleen cell suspensions. We verified that the absence of IL-23, IL-1R, or MyD88 inhibited, but did not block, the IL-17 production by ArtinM-stimulated spleen cells. Therefore, we investigated whether ArtinM exerts a direct effect on CD4+ T cells in promoting IL-17 production. Indeed, spleen cell suspensions depleted of CD4+ T cells responded to ArtinM with very low levels of IL-17 release. Likewise, isolated CD4+ T cells under ArtinM stimulus augmented the expression of TGF-β mRNA and released high levels of IL-17. Considering the observed synergism between IL-23 and ArtinM, we used cells from IL-23 KO mice to assess the direct effect of lectin on CD4+ T cells. We verified that ArtinM increased the IL-17 production significantly, a response that was inhibited when the CD4+ T cells were pre-incubated with anti-CD3 antibody. In conclusion, ArtinM stimulates the production

  11. IL-17 Induction by ArtinM is Due to Stimulation of IL-23 and IL-1 Release and/or Interaction with CD3 in CD4+ T Cells.

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    Thiago Aparecido da Silva

    Full Text Available ArtinM is a D-mannose-binding lectin extracted from the seeds of Artocarpus heterophyllus that interacts with TLR2 N-glycans and activates antigen-presenting cells (APCs, as manifested by IL-12 production. In vivo ArtinM administration induces Th1 immunity and confers protection against infection with several intracellular pathogens. In the murine model of Candida albicans infection, it was verified that, in addition to Th1, ArtinM induces Th17 immunity manifested by high IL-17 levels in the treated animals. Herein, we investigated the mechanisms accounting for the ArtinM-induced IL-17 production. We found that ArtinM stimulates the IL-17 production by spleen cells in BALB/c or C57BL/6 mice, a response that was significantly reduced in the absence of IL-23, MyD88, or IL-1R. Furthermore, we showed that ArtinM directly induced the IL-23 mRNA expression and the IL-1 production by macrophages. Consistently, in cell suspensions depleted of macrophages, the IL-17 production stimulated by ArtinM was reduced by 53% and the exogenous IL-23 acted synergistically with ArtinM in promoting IL-17 production by spleen cell suspensions. We verified that the absence of IL-23, IL-1R, or MyD88 inhibited, but did not block, the IL-17 production by ArtinM-stimulated spleen cells. Therefore, we investigated whether ArtinM exerts a direct effect on CD4+ T cells in promoting IL-17 production. Indeed, spleen cell suspensions depleted of CD4+ T cells responded to ArtinM with very low levels of IL-17 release. Likewise, isolated CD4+ T cells under ArtinM stimulus augmented the expression of TGF-β mRNA and released high levels of IL-17. Considering the observed synergism between IL-23 and ArtinM, we used cells from IL-23 KO mice to assess the direct effect of lectin on CD4+ T cells. We verified that ArtinM increased the IL-17 production significantly, a response that was inhibited when the CD4+ T cells were pre-incubated with anti-CD3 antibody. In conclusion, Artin