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Sample records for brazilian population sample

  1. Analysis of sagittal balance and spinopelvic parameters in a brazilian population sample

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    Raphael De Rezende Pratali

    2014-01-01

    Full Text Available Objective: To define the sagittal balance and spinopelvic parameters in a Brazilian population sample composed of asymptomatic volunteers. Methods: Fifty healthy adult individuals volunteered to participate in this study. The exclusion criteria included spinal deformity or improper radiograph study. In each radiograph exam in profile view, the sagittal balance and spinopelvic parameters were evaluated, including vertical sagittal axis, sacral slope, pelvic tilt and pelvic incidence. Results: The data obtained in this study are according to the values presented in the worldwide literature. None of the radiographic parameters showed any differences between the genders. Comparing the values presented in this study with those of other reports, with different populations, it was observed that: there was no significant difference in any of the pelvic parameters between Brazilian and Korean populations; there was a significant difference in pelvic incidence between Brazilian and European populations in a total sample (p=0.0001, in males (p=0.02, and in females (p=0.0007; there was a difference in sacral slope between Brazilian and European populations in a total sample (p=0.0140, and in females (p=0.005. Conclusion: There were no differences in any parameter in terms of gender. There were no differences in pelvic incidence between Brazilian and Korean populations. There was a difference in pelvic incidence between Brazilian and European populations, in the total sample and in males and females. There was a difference in sacral slope between Brazilian and European populations in a total sample, and in females.

  2. Estimate of dietary exposure to sulphites using Brazilian students as a sample population.

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    Popolim, W D; De V C Penteado, M

    2005-11-01

    In Brazil, there is neither a register of the use of sulphites by the food industry nor is research being undertaken on their dietary exposure to the population. The objective of the work reported here was to estimate the dietary exposure to sulphites in two different groups of high school students, a fee-paying school group and a state school group. The data were collected through a 24-hour dietary recall, which provided estimates of sulphited foods and beverages in the diet. The Maximum Permitted Level (MPL), established by the Brazilian legislation for each of the sulphited food and beverages, was used to measure the dietary exposure to this additive. On this basis none of the students could have exceeded the Acceptable Daily Intake (ADI) of 0.70 mg SO2/kg bw/day, with a average dietary exposure of 0.07 mg SO2/kg bw/day (p<0.001), with no significant statistical difference (p=0.643) between fee-paying and state school students. Highly exposed consumers (dietary exposure to more than 50% of the ADI, or either, 0.35 mg SO2/kg bw/day, to the maximum of 0.52 mg SO2/kg bw/dia) represented 4.5% of the researched samples and reached these levels of intake due to a consumption beyond 500 ml/day of industrialized packaged fruit juices, and, in the fee-paying school, for associating its consumption with alcoholic beverages like beer and wine.

  3. HLA-F coding and regulatory segments variability determined by massively parallel sequencing procedures in a Brazilian population sample.

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    Lima, Thálitta Hetamaro Ayala; Buttura, Renato Vidal; Donadi, Eduardo Antônio; Veiga-Castelli, Luciana Caricati; Mendes-Junior, Celso Teixeira; Castelli, Erick C

    2016-10-01

    Human Leucocyte Antigen F (HLA-F) is a non-classical HLA class I gene distinguished from its classical counterparts by low allelic polymorphism and distinctive expression patterns. Its exact function remains unknown. It is believed that HLA-F has tolerogenic and immune modulatory properties. Currently, there is little information regarding the HLA-F allelic variation among human populations and the available studies have evaluated only a fraction of the HLA-F gene segment and/or have searched for known alleles only. Here we present a strategy to evaluate the complete HLA-F variability including its 5' upstream, coding and 3' downstream segments by using massively parallel sequencing procedures. HLA-F variability was surveyed on 196 individuals from the Brazilian Southeast. The results indicate that the HLA-F gene is indeed conserved at the protein level, where thirty coding haplotypes or coding alleles were detected, encoding only four different HLA-F full-length protein molecules. Moreover, a same protein molecule is encoded by 82.45% of all coding alleles detected in this Brazilian population sample. However, the HLA-F nucleotide and haplotype variability is much higher than our current knowledge both in Brazilians and considering the 1000 Genomes Project data. This protein conservation is probably a consequence of the key role of HLA-F in the immune system physiology.

  4. Structural validity of the tonic immobility scale in a population exposed to trauma: evidence from two large Brazilian samples.

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    Michael Reichenheim

    Full Text Available BACKGROUND: Tonic Immobility is a temporary state of motor inhibition in situations involving extreme fear. The first scale developed for its assessment was the 10-item Tonic Immobility Scale (TIS. However, there are still few studies on its structural (dimensional validity. The objective of this study was to reassess the factor structure of the TIS applied to representative samples exposed to general trauma of two Brazilian mega-cities. METHODS: The sample comprised 3,223 participants reporting at least one traumatic experience. In São Paulo (n = 2,148, a Confirmatory Factor Analysis (CFA first tested the originally proposed two-dimensional structure. This was followed by sequential Exploratory Structural Equation Models to identify the best fitting model, and subsequently tested in Rio de Janeiro (n = 1,075 via CFA. Alternative reduced versions were further explored using the aggregate sample. Model-based Item Response Theory (IRT location parameters were also investigated. RESULTS: An absence of factor-based convergent and discriminant validity rejected the original proposition. However, the one-dimensional structure still held several residual correlations. Further exploration indicated the sustainability of reduced versions with seven (alternative A and six (alternative B items. Both presented excellent fit and no relevant residual item correlation. According to the IRT location parameters, items in alternative B covered a wider range of the latent trait. The Loevinger's H scalability coefficients underscored this pattern. CONCLUSIONS: The original model did not hold. A one-factor solution was the most tenable in both large samples, but with significant item residual correlations, indicating that content redundancies persisted. Further reduced and simplified versions of the TIS proved promising. Although studies are yet to be carried out in other settings, it is the authors' impression that the restricted versions of the TIS are

  5. Food cravings among Brazilian population.

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    Queiroz de Medeiros, Anna Cecília; Pedrosa, Lucia de Fatima Campos; Yamamoto, Maria Emilia

    2017-01-01

    This study aimed to develop and validate a Brazilian version of the Food Craving Inventory (FCI-Br), adapted to the cultural-gastronomic context of Brazil, and to explore this behavior among adult Brazilians. The Study 1 population consisted of 453 adults from all regions of Brazil. Participants responded to a preliminary form of the instrument online. Exploratory factor analysis revealed an FCI-Br presenting 23 items and three factors: High Fat, Sweet Food and Traditional Meal. The FCI-Br overall reliability was considered adequate (α = 0.82), as were each of the sub-scales. The food items receiving higher average scores from the application of the instrument were chocolate (3.14 ± 1.28; women) and bread (2.94 ± 1.44, men). A significant association was observed between the specific-craving for Sweet Food and female respondents. Most participants reported experiencing more frequent episodes of food craving when alone (68.0%; n = 391) and during the afternoon (32.2%; n = 127) or evening (43.8%; n = 173) hours. Application of the FCI-Br in a population of 649 university students (Study 2) demonstrated a good adjustment of the model developed according to the Confirmatory factor analysis (χ(2)/gl = 2.82, CFI = 0.94; TLI = 0.93; RMSEA = 0.06). The current findings indicate that the FCI-Br has adequate psychometric properties to measure craving behavior with respect to specific food groups in the resident population of Brazil. The results of this study also shed light on the importance of considering the cultural diversity of a population when investigating eating behaviors.

  6. Sleep complaints in the Brazilian population: Impact of socioeconomic factors

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    Camila Hirotsu

    2014-09-01

    Full Text Available National surveys are relevant for the study of sleep epidemiology since they can provide specific data about sleep in large dimension with important implications for the health system. Thus, the aim of this study was to investigate the prevalence of sleep complaints among the Brazilian population using a randomized cluster sample according to region and socioeconomic class. For this, a 3-stage sampling technique was used to randomly select Brazilian subjects of both genders older than 16 years. A total of 2017 subjects, from 132 different cities, were selected to estimate prevalence in the Brazilian population with a sampling error of ±2%. Questions about sleep complaints were administered face-to-face by Instituto Datafolha interviewers on April 10 and 16, 2012. Data were expanded using a weighted variable. The results showed that 76% of the study population suffers from at least 1 sleep complaint, indicating that approximately 108 million Brazilians may be affected by sleep disorders. On average, each subject had 1.9 sleep problems with the most common complaints being light and insufficient sleep, snoring, moving a lot during sleep, and insomnia, which usually occurred more than 3 times per week. Low income was associated with higher number of sleep complaints only in Northeast and Southeast regions. In conclusion, this study showed a high prevalence of sleep complaints in a sample of the Brazilian population, suggesting that sleep disorders may be markedly frequent in the Brazilian population with a possible correlation with the socioeconomic situation of the interviewed subjects.

  7. Development of two multiplex PCR systems for the analysis of 14 X-chromosomal STR loci in a southern Brazilian population sample.

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    Penna, Larissa Siqueira; Silva, Fernanda Gamio; Salim, Patricia Hartstein; Ewald, Gisele; Jobim, Mariana; Magalhães, José Antônio de Azevedo; Jobim, Luiz Fernando

    2012-03-01

    We developed two multiplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-Multiplex 1 consisted of DXS6807, DXS6800, DXS7424, DXS101, GATA172D05 and HPRTB and X-Multiplex 2 consisted of DXS8378, DXS9898, DXS6801, DXS6809, DXS6789, DXS7133, DXS8377 and DXS7423. In addition, we present allele frequencies for these loci in a south Brazilian population comprising 124 females and 141 males and haplotype frequencies of linked markers for males. Hardy-Weinberg equilibrium (HWE) was tested in the female sample and no significant deviations were found after applying Bonferroni's correction. Linkage disequilibrium (LD) tests were performed for all pairs of loci and three significant results, out of 91 pairwise comparisons, were obtained. We did not find any evidence of linkage disequilibrium between close or linked markers. The power of discrimination in females (PD(F)) varied between 0.832 for DXS6801 and 0.987 for DXS8377. DXS6801 was the least informative marker (PIC = 0.605), while DXS8377 was the most polymorphic (PIC = 0.911), followed by DXS101 (PIC = 0.872). Genetic distances were estimated for each STR marker applying the calculation of F (ST) between our total sample and other studies from Brazil, Europe, Asia and Africa. The most distant populations were Japan, Korea, China, Ghana and Uganda.

  8. p.Q192R SNP of PON1 seems not to be Associated with Carotid Atherosclerosis Risk Factors in an Asymptomatic and Normolipidemic Brazilian Population Sample

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    Daniel Zanetti Scherrer

    2015-07-01

    Full Text Available Background:Evidences suggest that paraoxonase 1 (PON1 confers important antioxidant and anti-inflammatory properties when associated with high-density lipoprotein (HDL.Objective:To investigate the relationships between p.Q192R SNP of PON1, biochemical parameters and carotid atherosclerosis in an asymptomatic, normolipidemic Brazilian population sample.Methods:We studied 584 volunteers (females n = 326, males n = 258; 19-75 years of age. Total genomic DNA was extracted and SNP was detected in the TaqMan® SNP OpenArray® genotyping platform (Applied Biosystems, Foster City, CA. Plasma lipoproteins and apolipoproteins were determined and PON1 activity was measured using paraoxon as a substrate. High-resolution β-mode ultrasonography was used to measure cIMT and the presence of carotid atherosclerotic plaques in a subgroup of individuals (n = 317.Results:The presence of p.192Q was associated with a significant increase in PON1 activity (RR = 12.30 (11.38; RQ = 46.96 (22.35; QQ = 85.35 (24.83 μmol/min; p Conclusion:In low-risk individuals, the presence of the p.192Q variant of PON1 is associated with a beneficial plasma lipid profile but not with carotid atherosclerosis.

  9. The impact of psychosocial factors on the risk of erectile dysfunction and inhibition of sexual desire in a sample of the Brazilian population

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    Carmita Helena Najjar Abdo

    Full Text Available CONTEXT: Sexual dysfunctions can have origins in physical, psychological and psychosocial factors. OBJECTIVE: To describe the frequency of erectile dysfunction (ED and female inhibition of sexual desire (ISD in a Brazilian sample, and to estimate the risks of these dysfunctions. TYPE OF STUDY: Non-random survey. SETTING: Ten Brazilian cities. METHODS: 2,835 subjects (53% women aged over 18 years answered a questionnaire about their general health and sex life. The chi-squared test and multivariate logistic regression were used. Values of p < 0.05 were considered significant. RESULTS: The women's average age was 36.6 years (± 13.3 and the men's was 39.5 (± 13.3. 14.7% of men presented moderate/complete ED and 34.6% of women presented ISD. Depression was mentioned by 16.8% of men and 29.7% of women. The chances of having ED and ISD were higher for subjects who had had lower school attainment. Lack of a job and depression gave rise to 1.5 times (95% CI: 1.0 - 2.3 and 1.9 times (95% CI: 1.2 - 3.0 greater chances of ED respectively. Compared with men aged up to 25 years, those aged 41-60 had 1.9 times (95% CI: 1.0 - 3.4 and those aged 61 and over had 5.4 times (95% CI: 2.3 - 12.6 greater risk of ED. For women, lack of a job gave rise to 1.5 times (95% CI: 1.1 - 1.9 greater chance of ISD; depression was not associated with higher risk. Compared with women aged up to 25 years, those aged 41-60 and 61 or over had, respectively, 2.9 times (95% CI: 2.0 - 4.1 and 7.5 times (95% CI: 3.0 -18.6 greater risk of ISD. DISCUSSION: Increasing unemployment has affected the whole population, but especially those with lower levels of school attainment. Such levels are positively linked with presence of sexual dysfunctions. CONCLUSION: Lack of jobs, age and low school attainment are risks for the sexual dysfunctions studied. Depression increased the risk of ED but not female ISD.

  10. Gender differences in the perception of asthma and respiratory symptoms in a population sample of asthma patients in four Brazilian cities

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    Laura Russo Zillmer

    2014-12-01

    Full Text Available OBJECTIVE: To evaluate the impact of asthma, by gender, in a population sample of asthma patients in Brazil. METHODS: We conducted face-to-face interviews with 400 subjects (> 12 years of age included in a national probability telephone sample of asthma patients in the Brazilian state capitals of São Paulo, Rio de Janeiro, Curitiba, and Salvador. Each of those 400 subjects completed a 53-item questionnaire that addressed five asthma domains: symptoms; impact of asthma on quality of life; perception of asthma control; exacerbations; and treatment/medication. RESULTS: Of the 400 patients interviewed, 272 (68% were female. In relation to respiratory symptoms, the proportion of women reporting extremely bothersome symptoms (cough with sputum, tightness in the chest, cough/shortness of breath/tightness in the chest during exercise, nocturnal shortness of breath, and nocturnal cough was greater than was that of men. Daytime symptoms, such as cough, shortness of breath, wheezing, and tightness in the chest, were more common among women than among men. Women also more often reported that their asthma interfered with normal physical exertion, social activities, sleep, and life in general. Regarding the impact of asthma on quality of life, the proportion of subjects who reported that asthma caused them to feel that they had no control over their lives and affected the way that they felt about themselves was also greater among women than among men. CONCLUSIONS: Among women, asthma tends to be more symptomatic, as well as having a more pronounced effect on activities of daily living and on quality of life.

  11. Dental anthropology of a Brazilian sample: Frequency of nonmetric traits.

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    Tinoco, Rachel Lima Ribeiro; Lima, Laíse Nascimento Correia; Delwing, Fábio; Francesquini, Luiz; Daruge, Eduardo

    2016-01-01

    Dental elements are valuable tools in a study of ancient populations and species, and key-features for human identification; among the dental anthropology field, nonmetric traits, standardized by ASUDAS, are closely related to ancestry. This study aimed to analyze the frequency of six nonmetric traits in a sample from Southeast Brazil, composed by 130 dental casts from individuals aged between 18 and 30, without foreign parents or grandparents. A single examiner observed the presence or absence of shoveling, Carabelli's cusp, fifth cusp, 3-cusped UM2, sixth cusp, and 4-cusped LM2. The frequencies obtained were different from the ones shown by other researches to Amerindian and South American samples, and related to European and sub-Saharan frequencies, showing the influence of this groups in the current Brazilian population. Sexual dimorphism was found in the frequencies of Carabelli's cusp, 3-cusped UM2, and sixth cusp.

  12. Application of the physiological and morphological parameters of the brazilian population sample to the mathematical model of the human respiratory tract; Aplicacao dos parametros fisiologicos e morfologicos de uma amostra da populacao brasileira no modelo matematico do trato respiratorio humano

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    Reis, Arlene Alves dos

    2005-07-01

    The Human Respiratory Tract Model proposed by the ICRP Publication 66 accounts for the morphology and physiology of the respiratory tract. The characteristics of air drawn into the lungs and exhaled are greatly influenced by the morphology of the respiratory tract, which causes numerous changes in pressure, flow rate, direction and humidity as air moves into and out of the lungs. Concerning the respiratory physiological parameters the breathing characteristics influence the volume, the inhalation rate of air and the portion that enters through the nose and the mouth. These characteristics are important to determine the fractional deposition. The model uses morphological and physiological parameters from the Caucasian man to establish deposition fractions in the respiratory tract regions. It is known that the morphology and physiology are influenced by environmental, occupational and economic conditions. The ICRP recommends, for a reliable evaluation of the regional deposition, the use of parameters from a local population when information is available. The main purpose of this study is to verify the influence in using the morphology and physiology parameters representative of a sample of the Brazilian population on the deposition model of the ICRP Publication 66. The morphological and physiological data were obtained from the literature. The software EXCEL for Windows (version 2000) was used in order to implement the deposition model and also to allow the changes in parameters of interest. Initially, the implemented model was checked using the parameters defined by the ICRP and the results of the fraction deposition in the respiratory tract compartments were compared. Finally, morphological and physiological parameters from Brazilian adult male were applied and the fractional deposition calculated. The results suggest a significant variation in fractional deposition when Brazilian parameters are applied in the model. (author)

  13. Frequency of CCR5delta32 in Brazilian populations

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    A.E. Vargas

    2006-03-01

    Full Text Available A sample of 103 randomly chosen healthy individuals from Alegrete, RS, Brazil, was tested for the CCR5delta32 allele, which is known to influence susceptibility to HIV-1 infection. The CCR5delta32 allele was identified by PCR amplification using specific primers flanking the region of deletion, followed by electrophoresis on a 3% agarose gel. The data obtained were compared to those reported for other populations and interpreted in terms of Brazilian history. The individuals studied came from a highly admixed population. Most of them were identified as white (N = 59, while blacks and browns (mulattoes were N = 13 and N = 31, respectively. The observed frequencies, considering the white, black and brown samples (6.8, 3.8, and 6.4%, respectively, suggest an important European parental contribution, even in populations identified as black and brown. However, in Brazil as a whole, this allele shows gradients indicating a relatively good correlation with the classification based on skin color and other physical traits, used here to define major Brazilian population groups.

  14. Dementia in Parkinson's disease: a Brazilian sample

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    Beatriz Baldivia

    2011-10-01

    Full Text Available To determine the prevalence of dementia associated with Parkinson's disease (PD-D in a Brazilian sample adopting clinical and diagnostic procedures recommended by the Movement Disorders Society (MDS. Sixty-seven patients were consecutively submitted to neurological, neuropsychological and functional examinations. PD-D was established according to MDS clinical criteria (Level II and clinical procedures for PD-D (Level I and prevalence rate was compared between the levels adopted. Ten patients (14.9% were diagnosed as demented by Level I criteria whereas sixteen (23.8% were diagnosed based on Level II criteria. Level I criteria had low sensitivity in detecting PD-D (31.25%, but greater specificity (90.19. The PD-D group had significantly worse performance on all neuropsychological tests, were older (p<0.001, had an older age of onset ofdisease (p<0.01, had lower educational level (p<0.02 and had higher scores on functional scales. Current age (p=0.046 and Hoehn & Yahr score (p=0.048 were predictors for developing PD-D.

  15. Determination of Amphetamine, Amfepramone and Fenproporex in Urine Samples by HPLC-DAD: Application to a Population of Brazilian Truck Drivers

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    Takitane,Juliana; Almeida, Rafael M.; Tiago F. de Oliveira; Prado,Natanael V.; Muñoz,Daniel R.; Leyton,Vilma; Yonamine, Mauricio

    2016-01-01

    Commercially available immunoassay tests are designed to detect the presence of amphetamine/methamphetamine or methylenodioxyamphetamines. However, it is known that Brazilian truck drivers also report the use of other illicit amphetamines, such as amfepramone and fenproporex. Thus, a method was developed and validated in order to quantify amphetamine-type stimulants (amphetamine, fenproporex and amfepramone) in urine by high performance liquid chromatography with diode array detection (HPLC-D...

  16. [Behaviors related to sunlight exposure versus protection in a random population sample from 15 Brazilian State capitals and the Federal District, 2002-2003].

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    Szklo, André Salem; Almeida, Liz Maria de; Figueiredo, Valeska; Lozana, José de Azevedo; Azevedo e Silva Mendonça, Gulnar; Moura, Lenildo de; Szklo, Moysés

    2007-04-01

    This article examines region-specific relations between prevalence of protection against sunlight and socio-demographic and behavioral variables in Brazil. Data were derived from a cross-sectional population-based random sample. Information on sunlight exposure was available for a total of 16,999 individuals 15 years and older. Comparing the North and South of Brazil, crude differences between women and men in the use of "sunscreen" and "protective headwear" were +10.9% (95%CI: 7.1; 14.6) and -11.6% (95%CI: -17.0; -6.3) in the North and +21.3% (95%CI: 17.7; 24.9) and -16.0% (95%CI: -20.2; -12.5) in the South. Adjusted differences by selected variables confirmed that women use more sunscreen protection and less headwear protection as compared to men in both the North and South, but the difference was not homogeneous by region (interaction term p value < 0.05).

  17. Prevalence of malocclusions in a young Brazilian population.

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    Grando, G; Young, A A A; Vedovello Filho, M; Vedovello, S A S; Ramirez-Yañez, G O

    2008-01-01

    Malocclusions are generally treated in adolescents and adults, but they are established at an early age. The purpose of this study was to determine the prevalence of malocclusions in a young Brazilian population. The sample included 926 children, 8 to 12 years old, attending 5 public schools in the state of Goias, Brazil The type of occlusion was visually determined during the oral exam and statistical analysis, Chi-square test, was performed to correlate the prevalence of malocclusion with gender and with age. 819 patients out of the 926 patients had some type of malocclusion. From those, 513 patients had a class I malocclusion, 201 patients were classified as class II malocclusion, and 105 patients were class III malocclusion. Vertically, 62 patients showed a deep bite and 61 patients had an open bite. Transversely, 40 patients presented a bilateral posterior crossbite, 54 patients had a posterior crossbite on the left side, and 39 patients had a posterior crossbite on the right side. No significant correlation between gender and malocclusions was found and the number of patients with malocclusions between boys and girls were similar. Considering the three spatial planes, there is a high prevalence of malocclusions among the young Brazilian population. Therefore, the dental community must improve health policies and treat malocclusions earlier.

  18. Huntington disease: DNA analysis in brazilian population

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    RASKIN SALMO

    2000-01-01

    Full Text Available Huntington disease (HD is associated with expansions of a CAG trinucleotide repeat in the HD gene. Accurate measurement of a specific CAG repeat sequence in the HD gene in 92 Brazilian controls without HD, 44 Brazilian subjects with clinical findings suggestive of HD and 40 individuals from 6 putative HD families, showed a range from 7 to 33 repeats in normal subjects and 39 to 88 repeats in affected subjects. A trend between early age at onset of first symptoms and increasing number of repeats was seen. Major increase of repeat size through paternal inheritance than through maternal inheritance was observed. Data generated from this study may have significant implications for the etiology, knowledge of the incidence, diagnosis, prognosis, genetic counseling and treatment of HD Brazilian patients.

  19. Prototypical faces from samples of the population of a Brazilian region / Faces prototípicas provenientes de amostras populacionais de uma região brasileira

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    Ana Irene Fonseca Mendes

    2009-01-01

    Full Text Available The goal of this study was to generate male and female prototypical faces, by morphing technique, from faces of people who were self-declared as white, "mixed race" and black in Ribeirão Preto-SP. Each prototypical face was generated from 64 face images of volunteers. Another sample of volunteers judged the typicality of color/race of the prototypical faces and the faces that composed them. The results suggested a concordance between the self-declared color/race and the judgments, indicating that the prototypical faces are representative of their respective categories of color/race. Applications of the prototypical faces in studies of face perception and face recognition were discussed.

  20. Faces prototípicas provenientes de amostras populacionais de uma região brasileira Prototypical faces from samples of the population of a Brazilian region

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    Ana Irene Fonseca Mendes

    2009-01-01

    Full Text Available O objetivo dessa pesquisa foi gerar, por meio de técnica de computação gráfica denominada morphing, faces prototípicas masculinas e femininas a partir de amostras de pessoas que se autodeclaram brancas, pardas e pretas na região de Ribeirão Preto (SP. A cada face prototípica foram utilizadas 64 imagens de faces de voluntários. As faces prototípicas e as faces que as compuseram tiveram a tipicidade de sua cor ou raça julgada por terceiros. Os julgamentos indicaram uma concordância entre a cor/raça-autodeclarada e a heteroatribuída o que indica que os protótipos são representativos de suas respectivas categorias de cor/raça. Discute-se como as faces prototípicas podem ser empregadas em pesquisas básicas e aplicadas em percepção e reconhecimento de faces.The goal of this study was to generate male and female prototypical faces, by morphing technique, from faces of people who were self-declared as white, "mixed race" and black in Ribeirão Preto-SP. Each prototypical face was generated from 64 face images of volunteers. Another sample of volunteers judged the typicality of color/race of the prototypical faces and the faces that composed them. The results suggested a concordance between the self-declared color/race and the judgments, indicating that the prototypical faces are representative of their respective categories of color/race. Applications of the prototypical faces in studies of face perception and face recognition were discussed.

  1. Demography, vulnerabilities and right to health to Brazilian prison population.

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    Soares, Marden Marques; Bueno, Paula Michele Martins Gomes

    2016-06-01

    This study investigates the latest research on the profile of the Brazilian prison population, its demography and current laws and regulations. It aims in the direction of ensuring the human right to health. Brazilian prison system is a complex universe in which state and federal criminal contexts keep more than 607,000 people in custody. This population is composed of 75% of young and black people, 67% poorly educated and 41% are pre-trial detainees, living in overcrowded prisons and architecturally vandalized, with population growth of around 575% in 24 years, making this environment a major focus of production of diseases. The prison becomes the object of differentiated intervention by public bodies linked to the executive and the judiciary - it is worth remarking that the data show the high level of inequalities and health vulnerabilities among the prison population, whose needs involve a set of cross-sector of transverse public policies actions towards penal execution.

  2. Sampling hard to reach populations.

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    Faugier, J; Sargeant, M

    1997-10-01

    Studies on 'hidden populations', such as homeless people, prostitutes and drug addicts, raise a number of specific methodological questions usually absent from research involving known populations and less sensitive subjects. This paper examines the advantages and limitations of nonrandom methods of data collection such as snowball sampling. It reviews the currently available literature on sampling hard to reach populations and highlights the dearth of material currently available on this subject. The paper also assesses the potential for using these methods in nursing research. The sampling methodology used by Faugier (1996) in her study of prostitutes, HIV and drugs is used as a current example within this context.

  3. Chemical profiling of six samples of Brazilian propolis

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    Caroline C. Fernandes-Silva

    2013-01-01

    Full Text Available Six samples of Brazilian propolis from Minas Gerais and Paraná states were analyzed to identify the constituents (GC/MS and HPLC/MS and to determine their contents (HPLC and external standardization. All samples contained characteristic constituents of green propolis, but the samples from Minas Gerais had higher contents of prenylated phenylpropanoids and caffeoylquinic acids. Kaempferide and two other flavonoids were among the major constituents of the samples from Minas Gerais. Luteolin 5-O-methyl ether was detected only in samples from Paraná. Baccharis dracunculifolia was a source of resins for all samples analyzed, but the samples from Paraná had more complex plant origin.

  4. Prevalence of burnout in a sample of Brazilian teachers

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    Pedro R. Gil-Monte

    2011-12-01

    Full Text Available Background and Objectives: Burnout is a psychological response to chronic work-related stress of an interpersonal and emotional nature. Brazilian law have already contemplated burnout syndrome as a mental and behavioural disorder related to work. The aim of this study was to identify the prevalence of burnout in a sample of Brazilian teachers. Methods: The sample was composed of 714 teachers from 8 schools in Porto Alegre and its metropolitan area (Brazil. The levels of burnout were evaluated by the Spanish Burnout Inventory, educational version (SBI-Ed. In addition, Psychosomatic disorders were estimated by the UNIPSICO subscale. Results: The percentage of participants who indicated high levels of burnout was 12 % (Profile 1, and 5.6% fell into Profile 2 because they were affected by strong feelings of guilt. Moreover, participants with high scores on the SBI dimensions (low on Enthusiasm toward the job scored significantly higher on psychosomatic disorders than participants with low scores. Conclusions: Based on psychometric considerations, participants who fit Profile 2 of burnout could be considered burnout cases according to Brazilian legislation. However, using a clinical interview to make the diagnosis is recommended.

  5. Genetic Diversity and Structure of Brazilian Populations of Diatraea saccharalis (Lepidoptera: Crambidae): Implications for Pest Management.

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    Silva-Brandão, Karina L; Santos, Thiago V; Cônsoli, Fernando L; Omoto, Celso

    2015-02-01

    The sugarcane borer, Diatraea saccharalis (F.), is the main pest of sugarcane in Brazil. Genetic variability and gene flow among 13 Brazilian populations of the species were evaluated based on mitochondrial DNA sequences to estimate the exchange of genetic information within and among populations. We found high genetic structure among sampled localities (ΦST=0.50923), and pairwise genetic distances were significantly correlated to geographic distances. Demographic analysis and genealogical network of mitochondrial sequences indicate population growth and admixture of D. saccharalis populations, events likely related to the sequential expansion of the corn and sugarcane crops in Brazil. The implications of these findings for pest management are discussed.

  6. Interethnic diversity of NAT2 polymorphisms in Brazilian admixed populations

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    Di Pietro Giuliano

    2010-10-01

    Full Text Available Abstract Background N-acetyltransferase type 2 (Nat2 is a phase II drug- metabolizing enzyme that plays a key role in the bioactivation of aromatic and heterocyclic amines. Its relevance in drug metabolism and disease susceptibility remains a central theme for pharmacogenetic research, mainly because of its genetic variability among human populations. In fact, the evolutionary and ethnic-specific SNPs on the NAT2 gene remain a focus for the potential discoveries in personalized drug therapy and genetic markers of diseases. Despite the wide characterization of NAT2 SNPs frequency in established ethnic groups, little data are available for highly admixed populations. In this context, five common NAT2 SNPs (G191A, C481T, G590A, A803G and G857A were investigated in a highly admixed population comprised of Afro-Brazilians, Whites, and Amerindians in northeastern Brazil. Thus, we sought to determine whether the distribution of NAT2 polymorphism is different among these three ethnic groups. Results Overall, there were no statistically significant differences in the distribution of NAT2 polymorphism when Afro-Brazilian and White groups were compared. Even the allele frequency of 191A, relatively common in African descendents, was not different between the Afro-Brazilian and White groups. However, allele and genotype frequencies of G590A were significantly higher in the Amerindian group than either in the Afro-Brazilian or White groups. Interestingly, a haplotype block between G590A and A803G was verified exclusively among Amerindians. Conclusions Our results indicate that ethnic admixture might contribute to a particular pattern of genetic diversity in the NAT2 gene and also offer new insights for the investigation of possible new NAT2 gene-environment effects in admixed populations.

  7. Population samples and genotyping technology.

    Science.gov (United States)

    Mack, S J; Sanchez-Mazas, A; Single, R M; Meyer, D; Hill, J; Dron, H A; Jani, A J; Thomson, G; Erlich, H A

    2007-04-01

    The 14th International HLA (human leukocyte antigen) Immunogenetics Workshop (14th-IHIWS) Biostatistics and Anthropology/Human Genetic Diversity project continues the population sampling, genotype data generation, and biostatistic analyses of the 13th International Histocompatibility Workshop Anthropology/Human Genetic Diversity Component, with the overall goal of further characterizing global HLA allele and haplotype diversity and better describing the relationships between major histocompatibility complex diversity, geography, linguistics, and population history. Since the 13th Workshop, new investigators have and continue to be recruited to the project and new high-resolution class I and class II genotype data are being generated for 112 population samples from around the world.

  8. Butyrylcholinesterase polymorphisms (BCHE and CHE2 loci) in Brazilian Indian and admixed populations.

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    Alcântara, V M; De Lourenço, M A; Salzano, F M; Petzl-Erler, M L; Coimbra, C E; Santos, R V; Chautard-Freire-Maia, E A

    1995-10-01

    The genetic variability of butyrylcholinesterase, determined by the BCHE and CHE2 loci, was examined in nine Brazilian Indian groups. In addition, a search for the presence of the BCHE*F allele was also performed in eight other Brazilian Indian samples and in five admixed (black-Indian-white) rural Amazonian communities previously studied for the CHE2 locus and the BCHE*A allele. In the Indian populations the frequency of the BCHE*F allele varied from 0 to 7.1% +/- 3.4 and the frequency of the CHE2 C5+ phenotype ranged from 1.4% +/- 1.4 to 45.9% +/- 3.8. This study seems to be the first to report the presence of the BCHE*F allele in native Americans. The BCHE*A allele appeared in one Indian group (1.4% +/- 1.0), and we suggest that its existence in this tribe and in other native Americans can be explained by gene flow from white populations. Gene flow may also be the reason for the occurrence of the BCHE*F allele in Brazilian Indians, whereas the CHE2*C5+ allele may have been present in the paleo-Indians. The distributions of both the BCHE*F allele and the CHE2 C5+ phenotype in Brazilian Indians seem to be the result of the action of random genetic drift.

  9. Childhood Trauma Questionnaire (CTQ) in Brazilian Samples of Different Age Groups: Findings from Confirmatory Factor Analysis

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    Grassi-Oliveira, Rodrigo; Cogo-Moreira, Hugo; Salum, Giovanni Abrahão; Brietzke, Elisa; Viola, Thiago Wendt; Manfro, Gisele Gus; Kristensen, Christian Haag; Arteche, Adriane Xavier

    2014-01-01

    The Childhood Trauma Questionnaire (CTQ) is internationally accepted as a key tool for the assessment of childhood abuse and neglect experiences. However, there are relative few psychometric studies available and some authors have proposed two different factor solutions. We examined the dimensional structure and internal consistency of the Brazilian version of the CTQ. A total of 1,925 participants from eight different clinical and non-clinical samples including adolescents, adults and elders were considered in this study. First, we performed Confirmatory Factor Analysis to investigate the goodness of fit of the two proposed competitive factor structure models for the CTQ. We also investigated the internal consistency of all factors. Second, multi-group analyses were used to investigate measurement invariance and population heterogeneity across age groups and sex. Our findings revealed that the alternative factor structure as opposed to the original factor structure was the most appropriate model within adolescents and adults Brazilian samples. We provide further evidence for the validity and reliability of the CTQ within the Brazilian samples and report that the alternative model showed an improvement in fit indexes and may be a better alternative over the original model. PMID:24475237

  10. Androgen receptor CAG polymorphism and the risk of benign prostatic hyperplasia in a Brazilian population

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    Vanderlei Biolchi

    2012-06-01

    Full Text Available Benign prostatic hyperplasia (BPH is a very frequent age-related proliferative abnormality in men. Polymorphic CAG repeat in the androgen receptor (AR can alter transactivation of androgen-responsive genes and potentially influence BPH risk. We investigated the association between CAG repeat length and risk of BPH in a case-control study of a Brazilian population. We evaluated 214 patients; 126 with BPH and 88 healthy controls. DNA was extracted from peripheral leucocytes and the AR gene was analyzed using fragment analysis. Hazard ratio (HR and 95% confidence interval were estimated using logistic regression models. Mean CAG length was not different between patients with BPH and controls. The CAG repeat length was examined as a categorical variable (CAG 21 and CAG 22 and did not differ between the control vs. the BPH group. We found no evidence for an association between AR CAG repeat length in BPH risk in a population-based sample of Brazilians.

  11. Estimate of consumption of phenolic compounds by Brazilian population

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    Vanesa Gesser Corrêa

    2015-04-01

    Full Text Available OBJECTIVE: Estimate the intake of phenolic compounds by the Brazilian population. METHODS: To estimate the average per capita food consumption, micro data from the National Dietary Survey and from the Household Budget Survey from 2008 to 2009 was analyzed. The phenolic content in food was estimated from the base of Phenol-Explorer. It was chosen according to compatibility and variety of food items and usual method of preparation. RESULTS: The Brazilian population consumed, on average, 460.15 mg/day of total phenolic compounds, derived mainly from beverages (48.9%, especially coffee and legumes (19.5%. Since this analysis of classes of phenolics it was possible to observe an intake of 314 mg/day of phenolic acids, 138.92 mg/day of flavonoids and 7.16 mg/ day of other kinds of phenolics. Regarding the variables studied this present study shows that those men who live in the countryside and in the northeastern region of the country had a higher consumption of phenolic compounds. Besides, consumption was higher by adults and the elderly, the medium income classes, the population with incomplete and complete primary education and those with adequate nutrition and also overweight status. CONCLUSION: The intake of phenolic compounds can be considered low, especially where consumption of fruit and vegetables is insufficient. We can conclude that coffee and black beans were the best contributors to phenolic intake.

  12. INFANTILE DRAWING AND IDENTITY BETWEEN MEXICAN INDIGENOUS POPULATIONS AND CENTER-WEST BRAZILIAN POPULATIONS

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    Sonia Grubits

    2011-01-01

    Full Text Available The aim of this article is to illustrate some fundamental aspects identified in the cultures of Brazilians indigenous social organizations and Mexicans indigenous organizations, start a short presentation of the more significant aspects of culture and the social organization for each chosen group and finally, a brief conclusion that shows similarity and differences between the referred groups of Brazil and Mexico, and identify some variables that are linked with infantile identity, this ones could be taken in the future to promote the child indigenous development. It is presented a data collected from center-west of Brazil, with infantile population (Bororo, Guarani and Kadiweu and three samples in Mexico, the first is with Mayo community in the north-west Mexico, others were from the south with Tzotsil and Nahualt people. We carried out participatory observation, interviews with families and analysis of drawings of the children, which show us similarities and differences among various native groups with the conformation of their ethnic identity. Center-west Brazilian sample it is possible notice high homogeneity, an integration among Kadiweu, and conflicts in the construction of the Guarani identity and between the Bororo people. Mayo and Tzotsil people of Mexico, their practices rituals abound the syncretism: in one hand is the catholic religion and in the other hand is the worship of the nature and the presence of both mixed during the pray, songs, dance, wear, etc. During observations in different communities and the analysis of infantile drawing, it was made evident the great influence since the first years of life from culture of the ethnic group and the non Indian culture in the building of the identity, social representations, etc. and often creating conflicts.

  13. Total Arsenic, Cadmium, and Lead Determination in Brazilian Rice Samples Using ICP-MS

    Science.gov (United States)

    Buzzo, Márcia Liane; de Arauz, Luciana Juncioni; Carvalho, Maria de Fátima Henriques; Arakaki, Edna Emy Kumagai; Matsuzaki, Richard; Tiglea, Paulo

    2016-01-01

    This study is aimed at investigating a suitable method for rice sample preparation as well as validating and applying the method for monitoring the concentration of total arsenic, cadmium, and lead in rice by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Various rice sample preparation procedures were evaluated. The analytical method was validated by measuring several parameters including limit of detection (LOD), limit of quantification (LOQ), linearity, relative bias, and repeatability. Regarding the sample preparation, recoveries of spiked samples were within the acceptable range from 89.3 to 98.2% for muffle furnace, 94.2 to 103.3% for heating block, 81.0 to 115.0% for hot plate, and 92.8 to 108.2% for microwave. Validation parameters showed that the method fits for its purpose, being the total arsenic, cadmium, and lead within the Brazilian Legislation limits. The method was applied for analyzing 37 rice samples (including polished, brown, and parboiled), consumed by the Brazilian population. The total arsenic, cadmium, and lead contents were lower than the established legislative values, except for total arsenic in one brown rice sample. This study indicated the need to establish monitoring programs for emphasizing the study on this type of cereal, aiming at promoting the Public Health.

  14. Total Arsenic, Cadmium, and Lead Determination in Brazilian Rice Samples Using ICP-MS

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    Lidiane Raquel Verola Mataveli

    2016-01-01

    Full Text Available This study is aimed at investigating a suitable method for rice sample preparation as well as validating and applying the method for monitoring the concentration of total arsenic, cadmium, and lead in rice by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS. Various rice sample preparation procedures were evaluated. The analytical method was validated by measuring several parameters including limit of detection (LOD, limit of quantification (LOQ, linearity, relative bias, and repeatability. Regarding the sample preparation, recoveries of spiked samples were within the acceptable range from 89.3 to 98.2% for muffle furnace, 94.2 to 103.3% for heating block, 81.0 to 115.0% for hot plate, and 92.8 to 108.2% for microwave. Validation parameters showed that the method fits for its purpose, being the total arsenic, cadmium, and lead within the Brazilian Legislation limits. The method was applied for analyzing 37 rice samples (including polished, brown, and parboiled, consumed by the Brazilian population. The total arsenic, cadmium, and lead contents were lower than the established legislative values, except for total arsenic in one brown rice sample. This study indicated the need to establish monitoring programs for emphasizing the study on this type of cereal, aiming at promoting the Public Health.

  15. Quality of life among the Brazilian adult population using the generic SF-8 questionnaire.

    Science.gov (United States)

    Campolina, Alessandro Gonçalves; Pinheiro, Marcelo M; Ciconelli, Rozana Mesquita; Ferraz, Marcos Bosi

    2011-06-01

    The objective of this study was to assess the quality of life in the Brazilian adult population, based on the U.S. standard population. It involved a cross-sectional population-based study with probabilistic sampling of 2,420 individuals (725 men and 1695 women) aged 40 or more in different geographic regions of Brazil. A socio-demographic questionnaire and the SF-8 (Short Form-8) were administered in interview form. Descriptive statistics, analysis of variance, the Mann-Whitney test and Tukey's test were used in the analysis. Females, populations in the northeastern region, the population of the regions of Brasília (Distrito Federal), Campo Grande (Mato Grosso do Sul State) and Goiania (Goiás State), Brazil, demonstrated worse quality of life. Age, education and income had influence over quality of life domains. This study presents quality of life estimates for the Brazilian adult population, based on the SF-8 questionnaire. The mean values on the subscales and components of the SF-8 appeared to be influenced by gender, geographic region, family income, age and schooling.

  16. SSR characterization of Oryza glumaepatula populations from the Brazilian Amazon and Cerrado biomes.

    Science.gov (United States)

    Abreu, Aluana Gonçalves; Rosa, Thalita Marra; Borba, Tereza Cristina de Oliveira; Vianello, Rosana Pereira; Rangel, Paulo Hideo Nakano; Brondani, Claudio

    2015-08-01

    The level and distribution of the genetic variability in 18 natural populations of Oryza glumaepatula that were collected from two Brazilian states were estimated using a set of 23 highly informative SSR markers. Samples comprising 78 and 117 individuals from populations of the states of Tocantins and Roraima, respectively, were evaluated in order to integrate and support previous studies that were carried out with populations of O. glumaepatula from Brazil. A total of 189 alleles were identified with an average of 8.22 alleles per locus. The 11 populations from Roraima presented, in combination, a higher genetic diversity (HE = 0.245) compared with that of the seven populations from Tocantins (HE = 0.212). All of the populations showed high and significant inbreeding values (mean f = 0.59); however, the mean was higher in Tocantins populations, indicating a higher gene flow in Roraima populations. The overall coefficient of genetic differentiation (FST) among the populations was high and significant (0.59) and was higher in Tocantins due to the isolation of each population, in contrast to Roraima, where gene flow occurred more frequently. The SSR panel used in this work resulted to be informative (polymorphism information content = 0.201) for assessing genetic structure in O. glumaepatula populations.

  17. Main visual symptoms associated to refractive errors and spectacle need in a Brazilian population

    Science.gov (United States)

    Schellini, Silvana; Ferraz, Fabio; Opromolla, Paula; Oliveira, Laryssa; Padovani, Carlos

    2016-01-01

    AIM To determine the main visual symptoms in a Brazilian population sample, associated to refractive errors (REs) and spectacle need to suggest priorities in preventive programs. METHODS A cross-sectional study was conducted in nine counties of the southeast region of Brazil, using a systematic sampling of households, between March 2004 and July 2005. The population was defined as individuals aged between 1 and 96y, inhabitants of 3600 residences to be evaluated and 3012 households were included, corresponding to 8010 subjects considered for participation in the survey, of whom 7654 underwent ophthalmic examinations. The individuals were evaluated according their demographic data, eye complaints and eye examination including the RE and the need to prescribe spectacles according to age. Statistical analysis was performed using SPSS software package and descriptive analysis using 95% confidence intervals (Pcorrect near vision. Subjects of ≥40 years old were determined to be at high risk of uncorrected REs. These observations can guide intervention programs for the Brazilian population. PMID:27990372

  18. Correspondence between overweight and socioeconomic and demographic indicators in the adult Brazilian population

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    Vladimir Schuindt da Silva

    2015-06-01

    Full Text Available OBJECTIVE: To verify the correlation between overweight and socioeconomic and demographic indicators among Brazilian adults, using data from the Brazilian Household Budget Survey from 2008/2009.METHODS: We analyzed the joint relationships between overweight and socioeconomic and demographic indicators in the Brazilian adult population (99,532,672 individuals, through the multiple correspondence analysis technique.RESULTS: The featured profile of the Brazilian adult population with regard to overweight was correlated with ages from 30 years of the most developed and economically social geographic regions of Brazil; however between genders, the correlation was in the opposite direction in the variables income, education, and subjective issues about life conditions related to food and nutrition security.CONCLUSION: By the joint relationship between overweight and selected demographic and socioeconomic indicators, the urgency of the development of strategies and/or preventive public health programs of health problems with overweight in the Brazilian adult population is suggested.

  19. Avaliação do desempenho dos testes functional reach e lateral reach em amostra populacional brasileira Assessment of performance in the functional reach and lateral reach tests in a Brazilian population sample

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    KRM Silveira

    2006-12-01

    Full Text Available OBJETIVOS: O objetivo deste estudo foi avaliar os padrões de desempenho dos testes Functional Reach e Lateral Reach em uma amostra de indivíduos saudáveis de 20 a 87 anos e verificar a influência do gênero, idade, estatura do indivíduo, peso corporal, comprimentos do braço e do pé. MÉTODO: foi realizado um estudo observacional transversal com 98 pessoas de ambos os gêneros, que residiam na capital e interior de São Paulo. Os voluntários tiveram suas medidas descritivas registradas e posteriormente foram submetidos aos testes Functional Reach e Lateral Reach. RESULTADOS: Para o FR, todas as variáveis tiveram influência, exceto o comprimento do braço (p=0,057, o peso corporal (p=0,746 e a base de suporte usada no momento da avaliação (p=0,384. As variáveis que exerceram maior influência foram o gênero (p=0,001, a idade (pOBJECTIVE: To assess the performance in the functional reach test (FR and lateral reach test (LR among a sample of healthy individuals aged 20 to 87 years and to verify the influence of gender, age, height, body weight, arm length and foot length. METHOD: A cross-sectional observational study was conducted on 98 people of both genders living in the city of São Paulo and other places in the State of São Paulo. The volunteers were measured and then underwent FR and LR. RESULTS: All the variables had an influence on FR, except arm length (p=0.057, body weight (p=0.746 and the support base used at the time of assessment (p=0.384. The variables exerting greatest influence were the individual's gender (p=0.001, age (p<0.001 and height (p=0.004. This analysis showed that women had less anterior and lateral functional reach than men. There was a substantial positive correlation (r=0.696 between the left and right LR findings. FR had a moderate positive correlation of 0.405 with the left LR and a substantial positive correlation of 0.614 with the right LR. For LR, the height, weight, foot length and arm length

  20. The Pemberton Happiness Index: Validation of the Universal Portuguese version in a large Brazilian sample.

    Science.gov (United States)

    Paiva, Bianca Sakamoto Ribeiro; de Camargos, Mayara Goulart; Demarzo, Marcelo Marcos Piva; Hervás, Gonzalo; Vázquez, Carmelo; Paiva, Carlos Eduardo

    2016-09-01

    The Pemberton Happiness Index (PHI) is a recently developed integrative measure of well-being that includes components of hedonic, eudaimonic, social, and experienced well-being. The PHI has been validated in several languages, but not in Portuguese. Our aim was to cross-culturally adapt the Universal Portuguese version of the PHI and to assess its psychometric properties in a sample of the Brazilian population using online surveys.An expert committee evaluated 2 versions of the PHI previously translated into Portuguese by the original authors using a standardized form for assessment of semantic/idiomatic, cultural, and conceptual equivalence. A pretesting was conducted employing cognitive debriefing methods. In sequence, the expert committee evaluated all the documents and reached a final Universal Portuguese PHI version. For the evaluation of the psychometric properties, the data were collected using online surveys in a cross-sectional study. The study population included healthcare professionals and users of the social network site Facebook from several Brazilian geographic areas. In addition to the PHI, participants completed the Satisfaction with Life Scale (SWLS), Diener and Emmons' Positive and Negative Experience Scale (PNES), Psychological Well-being Scale (PWS), and the Subjective Happiness Scale (SHS). Internal consistency, convergent validity, known-group validity, and test-retest reliability were evaluated. Satisfaction with the previous day was correlated with the 10 items assessing experienced well-being using the Cramer V test. Additionally, a cut-off value of PHI to identify a "happy individual" was defined using receiver-operating characteristic (ROC) curve methodology.Data from 1035 Brazilian participants were analyzed (health professionals = 180; Facebook users = 855). Regarding reliability results, the internal consistency (Cronbach alpha = 0.890 and 0.914) and test-retest (intraclass correlation coefficient = 0.814) were both considered

  1. Characterization of p53 gene mutations in a Brazilian population with oral squamous cell carcinomas.

    Science.gov (United States)

    Chaves, Anna C M; Cherubini, Karen; Herter, Nilton; Furian, Roque; Santos, Diogenes S; Squier, Christopher; Domann, Frederick E

    2004-02-01

    Mutations in the p53 tumor suppressor gene are present in approximately 50% of all human cancers. We sought to determine the frequency and type of p53 mutations in squamous cell carcinomas (SCC) of the oral cavity in a Brazilian population. To identify p53 mutations we used PCR-SSCP in tumor tissue microdissected from paraffin- embedded and from fresh-frozen sections followed by direct sequencing of SSCP bands with altered electrophoretic mobility. We identified p53 mutations in 40% of the human SCC analyzed. The mutations were of a broad spectrum, with a preponderance of G --> A and A --> G transitions with an apparent hotspot at the CpG dinucleotide at codon 290. Patient samples were stratified according to tobacco and alcohol consumption as well as by anatomic location of the tumor, and although trends did emerge, no statistically significant associations were obtained between the occurance of TP53 mutations and these lifestyle habits. We conclude that p53 mutations are common among oral cavity cancers in this population, and stress the significance of this study since it is the first analysis of p53 mutation in oral cancer in a southern Brazilian population.

  2. Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population

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    M.A.C. Smith

    2005-07-01

    Full Text Available Werner syndrome (WS is a premature aging disease caused by a mutation in the WRN gene. The gene was identified in 1996 and its product acts as a DNA helicase and exonuclease. Some specific WRN polymorphic variants were associated with increased risk for cardiovascular diseases. The identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. We investigated WRN codon 1367 polymorphism in 383 residents in a district of the city of São Paulo, who were enrolled in an Elderly Brazilian Longitudinal Study. Their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. This population was composed of 262 females (68.4% and 121 males (31.6% of European (89.2%, Japanese (3.3%, Middle Eastern (1.81%, and mixed and/or other origins (5.7%. There are no studies concerning this polymorphism in Brazilian population. These subjects were evaluated clinically every two years. The major health problems and morbidities affecting this cohort were cardiovascular diseases (21.7%, hypertension (83.7%, diabetes (63.3%, obesity (41.23%, dementia (8.0%, depression (20.0%, and neoplasia (10.8%. Their prevalence is similar to some urban elderly Brazilian samples. DNA was isolated from blood cells, amplified by PCR and digested with PmaCI. Allele frequencies were 0.788 for the cysteine and 0.211 for the arginine. Genotype distributions were within that expected for the Hardy-Weinberg equilibrium. Female gender was associated with hypertension and obesity. Logistic regression analysis did not detect significant association between the polymorphism and morbidity. These findings confirm those from Europeans and differ from Japanese population.

  3. Genetic diversity of four populations of Qualea grandiflora Mart. in fragments of the Brazilian Cerrado.

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    Antiqueira, Lia Maris Orth Ritter; Kageyama, Paulo Yoshio

    2014-02-01

    We analyzed the genetic structure and diversity of Qualea grandiflora Mart., the most abundant woody species in the Brazilian Cerrado. Eight microsatellite loci were used to analyze samples from four populations subjected to different types of anthropic pressure, distributed throughout the state of São Paulo in the regions of Assis, Brotas, Itirapina and Pedregulho. Results indicated a mean number of 12 alleles per locus, but only six effective alleles. Alleles private to particular populations and rare alleles were also detected. An excess of homozygotes and moderate levels of inbreeding were observed. No clones were identified. All populations departed from Hardy-Weinberg equilibrium (p < 0.05). Spatial structure was observed in the distribution of specimens in distance classes ranging from 30 to 40 km and three genetic clusters were identified, with genotypes in the Pedregulho population differing from the others by up to 90 %. The influence of the Wahlund effect on the studied populations lies between 8.5 and 53.3 %. Estimates of effective population size were low (<10), and the minimum viable area for conservation in the short-, medium- and long-term was estimated to be between 4 and 184 ha. Gene flow was high enough to counter the effects of genetic drift. The genetic diversity and divergence between the studied populations indicated that the Pedregulho population should be considered an Evolutionary Significant Unit and a Management Unit.

  4. First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest

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    Ana Carolina Srbek-Araujo

    Full Text Available Small and isolated wildlife populations may be more susceptible to disease, which makes illness an important issue to investigate regarding the conservation of large carnivores. Here, we present the results of the first investigation of intestinal parasites in one of the last remaining populations of jaguars in the Brazilian Atlantic Forest. We studied parasites from fecal samples using three different techniques for parasitological examination: floatation in saturated sodium chloride solution, sedimentation and formalin-ether centrifugation. Intestinal parasites were detected in 70% of the analyzed samples, and seven taxa (mean = 3.7 taxa/sample were identified. All the groups of parasites that were identified have been recorded in previous jaguar studies. However, the records of Class Trematoda and nematodes Trichuridae are the first evidence of these groups of worms in free-ranging jaguars in Brazil. Although our results do not provide conclusive evidence on the health of this jaguar population, given its very small size (approximately 20 animals we stress the need to properly understand the dynamics of disease in this wild population and to evaluate the risk of contracting new diseases from domestic species inhabiting the neighboring areas. These represent imperative actions for the successful conservation of this threatened population of jaguar.

  5. First record of intestinal parasites in a wild population of jaguar in the Brazilian Atlantic Forest.

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    Srbek-Araujo, Ana Carolina; Santos, Juliana Lúcia Costa; Almeida, Viviane Medeiros de; Guimarães, Marcos Pezzi; Chiarello, Adriano Garcia

    2014-01-01

    Small and isolated wildlife populations may be more susceptible to disease, which makes illness an important issue to investigate regarding the conservation of large carnivores. Here, we present the results of the first investigation of intestinal parasites in one of the last remaining populations of jaguars in the Brazilian Atlantic Forest. We studied parasites from fecal samples using three different techniques for parasitological examination: floatation in saturated sodium chloride solution, sedimentation and formalin-ether centrifugation. Intestinal parasites were detected in 70% of the analyzed samples, and seven taxa (mean = 3.7 taxa/sample) were identified. All the groups of parasites that were identified have been recorded in previous jaguar studies. However, the records of Class Trematoda and nematodes Trichuridae are the first evidence of these groups of worms in free-ranging jaguars in Brazil. Although our results do not provide conclusive evidence on the health of this jaguar population, given its very small size (approximately 20 animals) we stress the need to properly understand the dynamics of disease in this wild population and to evaluate the risk of contracting new diseases from domestic species inhabiting the neighboring areas. These represent imperative actions for the successful conservation of this threatened population of jaguar.

  6. Performance of an adult Brazilian sample on the Trail Making Test and Stroop Test

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    Kenia Repiso Campanholo

    Full Text Available ABSTRACT Objective: The Trail Making Test (TMT and Stroop Test (ST are attention tests widely used in clinical practice and research. The aim of this study was to provide normative data for the adult Brazilian population and to study the influence of gender, age and education on the TMT parts A and B, and ST cards A, B and C. Methods: We recruited 1447 healthy subjects aged ≥18 years with an educational level of 0-25 years who were native speakers of Portuguese (Brazilian. The subjects were evaluated by the Matrix Reasoning and Vocabulary subtests of the Wechsler Adult Intelligence Scale-III, along with the TMTA, TMTB and ST A, B and C. Results: Among the participants, mean intellectual efficiency was 103.20 (SD: 12.0, age 41.0 (SD: 16.4 years and education 11.9 (SD: 5.6 years. There were significant differences between genders on the TMTA (p=0.002, TMTB (p=0.017 and STC (p=0.024. Age showed a positive correlation with all attention tests, whereas education showed a negative correlation. Gender was not found to be significant on the multiple linear regression model, but age and education maintained their interference. Conclusion: Gender did not have the major impact on attentional tasks observed for age and education, both of which should be considered in the stratification of normative samples.

  7. Association of apolipoprotein E polymorphism with plasma lipids and Alzheimer's disease in a Southern Brazilian population

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    de-Andrade F.M.

    2000-01-01

    Full Text Available Apolipoprotein E (protein: apo E; gene: APOE plays an important role in the multifactorial etiology of both Alzheimer's disease (AD and lipid level concentrations. The polymerase chain reaction (PCR was used to investigate the APOE gene polymorphism in 446 unrelated Caucasians, among them 23 AD patients, and 100 Afro-Brazilians living in Porto Alegre, Brazil. The frequencies of the APOE*2, APOE*3 and APOE*4 alleles were 0.075, 0.810 and 0.115 in Caucasians and 0.075, 0.700 and 0.225 in Afro-Brazilians, respectively (c2 = 8.72, P = 0.013. A highly significant association was observed between the APOE*4 allele and AD in this population-based sample. The APOE*4 frequency in AD patients (39% was about four times higher than in the general Caucasian population (11.5%. The influence of each of the three common APOE alleles on lipid traits was evaluated by the use of the average excess statistic. The E*2 allele is associated with lower levels of triglycerides and of total and non-HDL cholesterol in both men and women. Conversely, the E*4 allele is associated with higher levels of these traits in women only. The effect of APOE alleles was of greater magnitude in women.

  8. Sampling Lesbian, Gay, and Bisexual Populations

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    Meyer, Ilan H.; Wilson, Patrick A.

    2009-01-01

    Sampling has been the single most influential component of conducting research with lesbian, gay, and bisexual (LGB) populations. Poor sampling designs can result in biased results that will mislead other researchers, policymakers, and practitioners. Investigators wishing to study LGB populations must therefore devote significant energy and…

  9. Genetic structuring among silverside fish (Atherinella brasiliensis) populations from different Brazilian regions

    Science.gov (United States)

    da Silva Cortinhas, Maria Cristina; Kersanach, Ralf; Proietti, Maíra; Dumont, Luiz Felipe Cestari; D'Incao, Fernando; Lacerda, Ana Luzia F.; Prata, Pedro Sanmartin; Matoso, Daniele Aparecida; Noleto, Rafael Bueno; Ramsdorf, Wanessa; Boni, Talge Aiex; Prioli, Alberto José; Cestari, Marta Margarete

    2016-09-01

    Estuaries are dynamic environments, key for the survival of innumerous ecologically or economically important fish species. Among these species are Neotropical silversides (Atherinella brasiliensis), which are resident and abundant in Brazilian estuaries and used as a complementary source of income and food for local communities. To better understand silverside populations in Brazil, we evaluated the genetic diversity, structure and demography of fish sampled at six estuaries from the northeastern to the southern coast, using Random Amplified Polymorphic DNA and mitochondrial DNA (D-loop) markers. High haplotype diversities (h ranging from 0.75 to 0.99) were found in all populations except Carapebus, located in Southeast Brazil (h = 0.54). A total of 69 mtDNA haplotypes were found, with Itaparica (Northeast Brazil) and Carapebus presenting only exclusive haplotypes, while some were shared among populations in the South. Strong regional structure was observed, with very high differentiation between Itaparica and Carapebus, as well as among these two populations and the ones from the Southern region (Paranaguá, Conceição, Camacho and Patos). Among southern areas, low/moderate structure was detected. Most populations showed unimodal mismatch distributions indicating recent demographic expansion, while Carapebus presented a multimodal distribution characteristic of a stable or bottlenecked population. Times since possible population expansion were highest in Itaparica (32,500 ya) and Carapebus (29,540 ya), while in the Southern region longest time was observed at Conceição (25,540 ya) and shortest at Patos (9720 ya). In a general manner, haplotype diversities were directly related to times since population expansions; again, Carapebus was the exception, displaying long time since expansion but low diversity, possibly due to a recent bottleneck caused by the isolation and human impacts this lagoon is subject to. Isolation by Distance was significant for Itaparica

  10. Indel markers: genetic diversity of 38 polymorphisms in Brazilian populations and application in a paternity investigation with post mortem material.

    Science.gov (United States)

    Manta, Fernanda; Caiafa, Alexandre; Pereira, Rui; Silva, Dayse; Amorim, António; Carvalho, Elizeu F; Gusmão, Leonor

    2012-09-01

    Aiming to evaluate the usefulness of 38 non-coding bi-allelic autosomal indels in genetic identification and kinship testing, three Brazilian population samples were studied: two from Rio de Janeiro (including a sample of individuals with self-declared African ancestry) and one Native American population of Terena from Mato Grosso do Sul. Based on the observed allele frequencies, parameters of forensic relevance were calculated. The combined power of discrimination of the 38 indels was high in all studied groups (PD≥0.9999999999997), although slightly lower in Native Americans. Genetic distance analysis showed significant differences between the allele frequencies in the Rio de Janeiro population and those previously reported for Europeans, Africans and Asians explained by its intermediate position between Europeans and Africans. As expected, the Terena sample was significantly different from all the other populations: Brazilians from Rio de Janeiro general population and with self-declared African ancestry, Europeans, Africans and East Asians. Finally, the performance of the 38-indel multiplex assay was tested in post-mortem material with positive results, supporting the use of short amplicon bi-allelic markers as an additional tool to STR analysis when DNA molecules are degraded.

  11. Dream characteristics in a Brazilian sample: an online survey focusing on lucid dreaming.

    Science.gov (United States)

    Mota-Rolim, Sérgio A; Targino, Zé H; Souza, Bryan C; Blanco, Wilfredo; Araujo, John F; Ribeiro, Sidarta

    2013-01-01

    During sleep, humans experience the offline images and sensations that we call dreams, which are typically emotional and lacking in rational judgment of their bizarreness. However, during lucid dreaming (LD), subjects know that they are dreaming, and may control oneiric content. Dreaming and LD features have been studied in North Americans, Europeans and Asians, but not among Brazilians, the largest population in Latin America. Here we investigated dreams and LD characteristics in a Brazilian sample (n = 3,427; median age = 25 years) through an online survey. The subjects reported recalling dreams at least once a week (76%), and that dreams typically depicted actions (93%), known people (92%), sounds/voices (78%), and colored images (76%). The oneiric content was associated with plans for the upcoming days (37%), memories of the previous day (13%), or unrelated to the dreamer (30%). Nightmares usually depicted anxiety/fear (65%), being stalked (48%), or other unpleasant sensations (47%). These data corroborate Freudian notion of day residue in dreams, and suggest that dreams and nightmares are simulations of life situations that are related to our psychobiological integrity. Regarding LD, we observed that 77% of the subjects experienced LD at least once in life (44% up to 10 episodes ever), and for 48% LD subjectively lasted less than 1 min. LD frequency correlated weakly with dream recall frequency (r = 0.20, p < 0.01), and LD control was rare (29%). LD occurrence was facilitated when subjects did not need to wake up early (38%), a situation that increases rapid eye movement sleep (REMS) duration, or when subjects were under stress (30%), which increases REMS transitions into waking. These results indicate that LD is relatively ubiquitous but rare, unstable, difficult to control, and facilitated by increases in REMS duration and transitions to wake state. Together with LD incidence in USA, Europe and Asia, our data from Latin America strengthen the notion that LD

  12. Dream characteristics in a Brazilian sample: an online survey focusing on lucid dreaming

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    Sérgio Arthuro Mota-Rolim

    2013-12-01

    Full Text Available During sleep, humans experience the offline images and sensations that we call dreams, which are typically emotional and lacking in rational judgment of their bizarreness. However, during lucid dreaming (LD, subjects know that they are dreaming, and may control oneiric content. Dreaming and LD features have been studied in North Americans, Europeans and Asians, but not among Brazilians, the largest population in Latin America. Here we investigated dreams and LD characteristics in a Brazilian sample (n=3,427; median age=25 years through an online survey. The subjects reported recalling dreams at least once a week (76%. Dreams typically depicted actions (93%, known people (92%, sounds/voices (78%, and colored images (76%. The oneiric content was associated with plans for the upcoming days (37%, memories of the previous day (13%, or unrelated to the dreamer (30%. Nightmares usually depicted anxiety/fear (65%, being stalked (48%, or other unpleasant sensations (47%. These data corroborate Freudian notion of day residue, and suggest that dreams are simulations of life situations that are related to our psychobiological integrity. Regarding LD, we observed that 77% of the subjects experienced LD at least once in life (44% up to 10 episodes ever, and for 48% LD subjectively lasted less than 1 minute. LD frequency correlated weakly with dream recall frequency (r=0.20, p<0.01, and LD control was rare (29%. LD occurrence was facilitated when subjects did not need to wake up early (38%, a situation that increases REMS duration, or when subjects were under stress (30%, which increases REMS transitions into waking. These results indicate that LD is a relatively ubiquitous but not frequent state, being unstable, difficult to control, and facilitated by increases in REMS duration and transitions to wake state. Together with LD incidence in USA, Europe and Asia, our data from Latin America strengthen the notion that LD is a general phenomenon of the human

  13. Exploring the distribution of genetic markers of pharmacogenomics relevance in Brazilian and Mexican populations.

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    Vania Bonifaz-Peña

    Full Text Available Studies of pharmacogenomics-related traits are increasingly being performed to identify loci that affect either drug response or susceptibility to adverse drug reactions. However, the effect of the polymorphisms can differ in magnitude or be absent depending on the population being assessed. We used the Affymetrix Drug Metabolizing Enzymes and Transporters (DMET Plus array to characterize the distribution of polymorphisms of pharmacogenetics and pharmacogenomics (PGx relevance in two samples from the most populous Latin American countries, Brazil and Mexico. The sample from Brazil included 268 individuals from the southeastern state of Rio de Janeiro, and was stratified into census categories. The sample from Mexico comprised 45 Native American Zapotecas and 224 self-identified Mestizo individuals from 5 states located in geographically distant regions in Mexico. We evaluated the admixture proportions in the Brazilian and Mexican samples using a panel of Ancestry Informative Markers extracted from the DMET array, which was validated with genome-wide data. A substantial variation in ancestral proportions across census categories in Brazil, and geographic regions in Mexico was identified. We evaluated the extent of genetic differentiation (measured as FST values of the genetic markers of the DMET Plus array between the relevant parental populations. Although the average levels of genetic differentiation are low, there is a long tail of markers showing large frequency differences, including markers located in genes belonging to the Cytochrome P450, Solute Carrier (SLC and UDP-glucuronyltransferase (UGT families as well as other genes of PGx relevance such as ABCC8, ADH1A, CHST3, PON1, PPARD, PPARG, and VKORC1. We show how differences in admixture history may have an important impact in the distribution of allele and genotype frequencies at the population level.

  14. Exploring the Distribution of Genetic Markers of Pharmacogenomics Relevance in Brazilian and Mexican Populations

    Science.gov (United States)

    Bonifaz-Peña, Vania; Contreras, Alejandra V.; Struchiner, Claudio Jose; Roela, Rosimeire A.; Furuya-Mazzotti, Tatiane K.; Chammas, Roger; Rangel-Escareño, Claudia; Uribe-Figueroa, Laura; Gómez-Vázquez, María José; McLeod, Howard L.; Hidalgo-Miranda, Alfredo

    2014-01-01

    Studies of pharmacogenomics-related traits are increasingly being performed to identify loci that affect either drug response or susceptibility to adverse drug reactions. However, the effect of the polymorphisms can differ in magnitude or be absent depending on the population being assessed. We used the Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus array to characterize the distribution of polymorphisms of pharmacogenetics and pharmacogenomics (PGx) relevance in two samples from the most populous Latin American countries, Brazil and Mexico. The sample from Brazil included 268 individuals from the southeastern state of Rio de Janeiro, and was stratified into census categories. The sample from Mexico comprised 45 Native American Zapotecas and 224 self-identified Mestizo individuals from 5 states located in geographically distant regions in Mexico. We evaluated the admixture proportions in the Brazilian and Mexican samples using a panel of Ancestry Informative Markers extracted from the DMET array, which was validated with genome-wide data. A substantial variation in ancestral proportions across census categories in Brazil, and geographic regions in Mexico was identified. We evaluated the extent of genetic differentiation (measured as FST values) of the genetic markers of the DMET Plus array between the relevant parental populations. Although the average levels of genetic differentiation are low, there is a long tail of markers showing large frequency differences, including markers located in genes belonging to the Cytochrome P450, Solute Carrier (SLC) and UDP-glucuronyltransferase (UGT) families as well as other genes of PGx relevance such as ABCC8, ADH1A, CHST3, PON1, PPARD, PPARG, and VKORC1. We show how differences in admixture history may have an important impact in the distribution of allele and genotype frequencies at the population level. PMID:25419701

  15. Exploring the distribution of genetic markers of pharmacogenomics relevance in Brazilian and Mexican populations.

    Science.gov (United States)

    Bonifaz-Peña, Vania; Contreras, Alejandra V; Struchiner, Claudio Jose; Roela, Rosimeire A; Furuya-Mazzotti, Tatiane K; Chammas, Roger; Rangel-Escareño, Claudia; Uribe-Figueroa, Laura; Gómez-Vázquez, María José; McLeod, Howard L; Hidalgo-Miranda, Alfredo; Parra, Esteban J; Fernández-López, Juan Carlos; Suarez-Kurtz, Guilherme

    2014-01-01

    Studies of pharmacogenomics-related traits are increasingly being performed to identify loci that affect either drug response or susceptibility to adverse drug reactions. However, the effect of the polymorphisms can differ in magnitude or be absent depending on the population being assessed. We used the Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus array to characterize the distribution of polymorphisms of pharmacogenetics and pharmacogenomics (PGx) relevance in two samples from the most populous Latin American countries, Brazil and Mexico. The sample from Brazil included 268 individuals from the southeastern state of Rio de Janeiro, and was stratified into census categories. The sample from Mexico comprised 45 Native American Zapotecas and 224 self-identified Mestizo individuals from 5 states located in geographically distant regions in Mexico. We evaluated the admixture proportions in the Brazilian and Mexican samples using a panel of Ancestry Informative Markers extracted from the DMET array, which was validated with genome-wide data. A substantial variation in ancestral proportions across census categories in Brazil, and geographic regions in Mexico was identified. We evaluated the extent of genetic differentiation (measured as FST values) of the genetic markers of the DMET Plus array between the relevant parental populations. Although the average levels of genetic differentiation are low, there is a long tail of markers showing large frequency differences, including markers located in genes belonging to the Cytochrome P450, Solute Carrier (SLC) and UDP-glucuronyltransferase (UGT) families as well as other genes of PGx relevance such as ABCC8, ADH1A, CHST3, PON1, PPARD, PPARG, and VKORC1. We show how differences in admixture history may have an important impact in the distribution of allele and genotype frequencies at the population level.

  16. HEPATITIS B VIRUS INFECTION PROFILE IN CENTRAL BRAZILIAN HEMODIALYSIS POPULATION

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    TELES Sheila A.

    1998-01-01

    Full Text Available Hepatitis B has proved to be a major health hazard in hemodialysis patients. In order to investigate the hepatitis B virus (HBV infection profile in the hemodialysis population of Goiânia city - Central Brazil, all dialysis patients (N=282 were studied. The prevalence of any HBV marker (HBsAg, anti-HBs, and anti-HBc was 56.7% (95% CI: 51.1-62.7, ranging from 33.3% to 77.7% depending on dialysis unit. HBV-DNA was detected in 67.6% and 88.2% of the HBsAg-positive serum samples, in 91.3% and 100% of the HBsAg/HBeAg-positive samples, and in 18.2% and 63.6% of the HBsAg/anti-HBe-reactive sera by hybridization and PCR, respectively. The length of time on hemodialysis was significantly associated with HBV seropositivity. Only 10% of the patients reported received hepatitis B vaccination. The findings of a high HBV infection prevalence in this population and the increased risk for HBV infection on long-term hemodialysis suggest the environmental transmission, emphasizing the urgent need to evaluate strategies of control and prevention followed in these units.

  17. Population Education in Geography: Some Sample Lessons.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    This booklet contains sample lessons and learning materials from the countries of Asia and Oceania for teaching population education in geography courses. The booklet is one of a series of six, each of which brings out population education concepts as part of a particular subject area. The subject areas treated in the other booklets are home…

  18. Complementary data on four methods for sampling free-living ticks in the Brazilian Pantanal.

    Science.gov (United States)

    Ramos, Vanessa do Nascimento; Osava, Carolina Fonseca; Piovezan, Ubiratan; Szabó, Matias Pablo Juan

    2014-01-01

    In this study, four methods for sampling free-living ticks that are used in ecological and human tick-bite risk studies were evaluated. Cloth dragging, carbon dioxide traps and visual searches and inspection of plant litter on the ground were used in field and forest areas within the Brazilian Pantanal. Among the three tick species collected, Amblyomma sculptum predominated, followed by Amblyomma parvum and Amblyomma ovale. Dragging, a cheap and simple technique, yielded the highest numbers of ticks, particularly nymphs. The visual search detected a high number of adult ticks and provided information on tick questing height. Even though laborious, plant litter examination showed that large numbers of ticks may use this stratum. Carbon dioxide (CO2) traps are expensive and difficult to handle, but they are highly efficient for adult ticks, especially A. parvum. These data indicate that one method alone is incapable of providing a representative sample of the tick fauna in a particular area and that multiple techniques should be used for tick population studies.

  19. The frequency of Tay-Sachs disease causing mutations in the Brazilian Jewish population justifies a carrier screening program

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    Roberto Rozenberg

    Full Text Available CONTEXT: Tay-Sachs disease is an autosomal recessive disease characterized by progressive neurologic degeneration, fatal in early childhood. In the Ashkenazi Jewish population the disease incidence is about 1 in every 3,500 newborns and the carrier frequency is 1 in every 29 individuals. Carrier screening programs for Tay-Sachs disease have reduced disease incidence by 90% in high-risk populations in several countries. The Brazilian Jewish population is estimated at 90,000 individuals. Currently, there is no screening program for Tay-Sachs disease in this population. OBJECTIVE: To evaluate the importance of a Tay-Sachs disease carrier screening program in the Brazilian Jewish population by determining the frequency of heterozygotes and the acceptance of the program by the community. SETTING: Laboratory of Molecular Genetics - Institute of Biosciences - Universidade de São Paulo. PARTICIPANTS: 581 senior students from selected Jewish high schools. PROCEDURE: Molecular analysis of Tay-Sachs disease causing mutations by PCR amplification of genomic DNA, followed by restriction enzyme digestion. RESULTS: Among 581 students that attended educational classes, 404 (70% elected to be tested for Tay-Sachs disease mutations. Of these, approximately 65% were of Ashkenazi Jewish origin. Eight carriers were detected corresponding to a carrier frequency of 1 in every 33 individuals in the Ashkenazi Jewish fraction of the sample. CONCLUSION: The frequency of Tay-Sachs disease carriers among the Ashkenazi Jewish population of Brazil is similar to that of other countries where carrier screening programs have led to a significant decrease in disease incidence. Therefore, it is justifiable to implement a Tay-Sachs disease carrier screening program for the Brazilian Jewish population.

  20. Screening for thyroid disorders in asymptomatic adults from Brazilian populations

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    Isabela Benseñor

    Full Text Available Advances in thyroid disorder diagnosis have created new thyroid disorder categories such as subclinical hyperthyroidism and subclinical hypothyroidism. In the 1980s, immunometric assaying for thyroid stimulating hormone (TSH emerged and became defined as the most cost-effective test in thyroid disorder screening. The second step in the screening of thyroid disorders is to determine free thyroxine (FT4, and cost-effective methods for its detection are now available. Using TSH and FT4, it is possible to determine four situations: clinical hyperthyroidism, clinical hypothyroidism, subclinical hyperthyroidism and subclinical hypothyroidism. Subclinical hypothyroidism can be a strong indicator of risk for atherosclerosis and myocardial infarction in elderly women. Cardiovascular mortality among Brazilian women is one of the highest in the Western world. The best-known risk factors for cardiovascular diseases are high blood pressure, smoking, diabetes, and hypercholesterolemia. Although these are recognized as primary risk factors, there are other risk factors that could be identified as primordial risk factors. This may be the case for subclinical hypothyroidism. Early detection of thyroid disorders in women over fifty could be a highly cost-effective option in the prevention of cardiovascular disorders among Brazilian women.

  1. [Prevalence of depressive symptoms and associated factors among southern Brazilian adults: cross-sectional population-based study].

    Science.gov (United States)

    Rombaldi, Airton José; da Silva, Marcelo Cozzensa; Gazalle, Fernando Kratz; Azevedo, Mario Renato; Hallal, Pedro Curi

    2010-12-01

    To identify the prevalence of depressive symptoms and examine associated factors in a Southern Brazilian adult population, a cross-sectional population-based study was carried out, including 972 subjects, men and women, aged 20 to 69 years, living in the urban area of Pelotas, Rio Grande do Sul. The sampling strategy relied on the census tracts of the city as primary sampling units and households as the secondary units. The questionnaire included socio-demographic, behavioral and nutritional variables. The prevalence of sadness, anxiety, loss of energy, lack of will to do things, thinking about the past, and wishing to stay at home were 29.4%, 57.6%, 37.4%, 40.4%, 33.8%, and 54.3%, respectively. Female gender, older ages, smokers and obese individuals showed association with depressive symptoms. Population-based studies using longitudinal designs may help to clarify the relationship between biopsychosocial variables and depressive symptoms.

  2. Determination of insulin-like growth factor-I reference values using an immunoradiometric assay in a Brazilian adult population

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    Denise B Leite

    2012-01-01

    Full Text Available Background: Serum levels of total insulin-like growth factor I (IGF-I reflect endogenous growth hormone (GH secretion in healthy adults, which makes it a good diagnostic marker for screening of GH-related disorders. Studies also have supported a possible relation between IGF-I levels and the risk and prognostic for some malignancies, besides a relation between IGF-I levels and mortality. Objective: As the determination of the IGF-I normal values for local populations is strongly desired, the aim of this investigation was to determine reference values for IGF-I using an immunoradiometric assay (IRMA in an adult Brazilian population of Rio de Janeiro city, since there is no other study using this methodology in Brazilian population, and that this method is widely used in Brazil and worldwide. Materials and Methods: The study included samples of blood taken from 484 healthy subjects (251 men and 233 women aged 18-70. The subjects agreed with this study, approved by the Ethical Committee of the Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti, Rio de Janeiro, Brazil. The samples were analyzed using a Diagnostic System Laboratories kit. For data analysis, age- and sex-specific figures were fitted after transformation of IGF-I values. Results: In adulthood, a slow age-dependent decrease was found. There was no significant difference in IGF-I values between men and women. Conclusion: This study established age-specific IGF-I reference values, for a healthy Brazilian adult population, determined by a widely IGF-I, IRMA used currently in Brazil.

  3. Distribution of QPY and RAH haplotypes of granzyme B gene in distinct Brazilian populations

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    Fernanda Bernadelli Garcia

    2012-08-01

    Full Text Available INTRODUCTION: The cytolysis mediated by granules is one of the most important effector functions of cytotoxic T lymphocytes and natural killer cells. Recently, three single nucleotide polymorphisms (SNPs were identified at exons 2, 3, and 5 of the granzyme B gene, resulting in a haplotype in which three amino acids of mature protein Q48P88Y245 are changed to R48A88H245, which leads to loss of cytotoxic activity of the protein. In this study, we evaluated the frequency of these polymorphisms in Brazilian populations. METHODS: We evaluated the frequency of these polymorphisms in Brazilian ethnic groups (white, Afro-Brazilian, and Asian by sequencing these regions. RESULTS: The allelic and genotypic frequencies of SNP 2364A/G at exon 2 in Afro-Brazilian individuals (42.3% and 17.3% were significantly higher when compared with those in whites and Asians (p < 0.0001 and p = 0.0007, respectively. The polymorphisms 2933C/G and 4243C/T also were more frequent in Afro-Brazilians but without any significant difference regarding the other groups. The Afro-Brazilian group presented greater diversity of haplotypes, and the RAH haplotype seemed to be more frequent in this group (25%, followed by the whites (20.7% and by the Asians (11.9%, similar to the frequency presented in the literature. CONCLUSIONS: There is a higher frequency of polymorphisms in Afro-Brazilians, and the RAH haplotype was more frequent in these individuals. We believe that further studies should aim to investigate the correlation of this haplotype with diseases related to immunity mediated by cytotoxic lymphocytes, and if this correlation is confirmed, novel treatment strategies might be elaborated.

  4. Efficiency of snake sampling methods in the Brazilian semiarid region.

    Science.gov (United States)

    Mesquita, Paula C M D; Passos, Daniel C; Cechin, Sonia Z

    2013-09-01

    The choice of sampling methods is a crucial step in every field survey in herpetology. In countries where time and financial support are limited, the choice of the methods is critical. The methods used to sample snakes often lack objective criteria, and the traditional methods have apparently been more important when making the choice. Consequently researches using not-standardized methods are frequently found in the literature. We have compared four commonly used methods for sampling snake assemblages in a semiarid area in Brazil. We compared the efficacy of each method based on the cost-benefit regarding the number of individuals and species captured, time, and financial investment. We found that pitfall traps were the less effective method in all aspects that were evaluated and it was not complementary to the other methods in terms of abundance of species and assemblage structure. We conclude that methods can only be considered complementary if they are standardized to the objectives of the study. The use of pitfall traps in short-term surveys of the snake fauna in areas with shrubby vegetation and stony soil is not recommended.

  5. [Cross-cultural adaptation of the Tilburg Frailty Indicator (TFI) for use in the Brazilian population].

    Science.gov (United States)

    Santiago, Lívia Maria; Luz, Laércio Lima; Mattos, Inês Echenique; Gobbens, Robbert J J

    2012-09-01

    The current study aimed to adapt the Tilburg Frailty Indicator (TFI), used to assess frailty in the elderly, to the Brazilian population. Conceptual, item, and semantic equivalences were analyzed and the summary version was pre-tested. In the evaluation of conceptual equivalence, the construct for frailty adopted in Brazil demonstrated the same conceptualization as in other cultures where this condition has been investigated. All items included in the original version also showed similarity in the two cultures. High semantic equivalence was observed in the analysis of the items' referential and general meanings. The pre-test showed a high percentage of understanding of items and good acceptance of items by elderly individuals. The results suggest that the TFI version adapted to the Brazilian culture can be a useful tool for assessing health conditions in older Brazilians.

  6. SLC24A5 and ASIP as phenotypic predictors in Brazilian population for forensic purposes.

    Science.gov (United States)

    Lima, F A; de Araújo Lima, Felícia; Gonçalves, F T; de Toledo Gonçalves, Fernanda; Fridman, C; Fridman, Cintia

    2015-07-01

    Pigmentation is a variable and complex trait in humans and it is determined by the interaction of environmental factors, age, disease, hormones, exposure to ultraviolet radiation and genetic factors, including pigmentation genes. Many polymorphisms of these genes have been associated with phenotypic diversity of skin, eyes and hair color in homogeneous populations. Phenotype prediction from biological samples using genetic information has benefited forensic area in some countries, leading some criminal investigations. Herein, we evaluated the association between polymorphisms in the genes SLC24A5 (rs1426654) and ASIP (rs6058017) with skin, eyes and hair colors, in 483 healthy individuals from Brazilian population for attainable use in forensic practice. The volunteers answered a questionnaire where they self-reported their skin, eye and hair colors. The polymorphic homozygous genotype of rs1426654∗A and rs6058017∗A in SLC24A5 and ASIP respectively, showed strongest association with fairer skin (OR 47.8; CI 14.1-161.6 and OR 8.6; CI 2.5-29.8); SLC24A5 alone showed associations with blue eyes (OR 20.7; CI 1.2-346.3) and blond hair (OR 26.6; CI 1.5-460.9). Our data showed that polymorphic genotypes (AA), in both genes, are correlated with characteristics of light pigmentation, while the ancestral genotype (GG) is related to darker traits, corroborating with previous studies in European and African populations. These associations show that specific molecular information of an individual may be useful to access some phenotypic features in an attempt to help forensic investigations, not only on crime scene samples but also in cases of face reconstructions in unknown bodies.

  7. Elaboração e validação de intervalos de referência longitudinais de peso fetal com uma amostra da população brasileira Elaboration and validation of longitudinal reference intervals of fetal weight with a sample of the Brazilian population

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    Érica Luciana de Paula Furlan

    2012-10-01

    Full Text Available OBJETIVOS: Elaborar modelos de predição de peso fetal e de percentis longitudinais de peso fetal estimado (PFE com uma amostra da população brasileira. MÉTODOS: Estudo observacional prospectivo. Dois grupos de gestantes foram recrutados: Grupo EPF (estimativa de peso fetal: pacientes para elaboração (EPF-El e validação (EPF-Val de um modelo de predição de peso fetal; Grupo IRL (intervalos de referência longitudinais: gestantes para elaboração (IRL-El e validação (IRL-Val de intervalos de referência longitudinais de PFE. Regressão polinomial foi utilizada com os dados do subgrupo EPF-El para gerar o modelo de predição de peso fetal. O desempenho deste modelo foi comparado com os de outros disponíveis na literatura. Modelos lineares mistos foram usados para elaboração de intervalos longitudinais de PFE com os dados do subgrupo IRL-El. Os dados do subgrupo IRL-Val foram usados para validação destes intervalos. RESULTADOS: Quatrocentos e cinqüenta e oito pacientes compuseram o Grupo EPF (EPF-El: 367; EPF-Val: 91 e 315 o Grupo IRL (IRL-El: 265; IRL-Val: 50. A fórmula para cálculo do PFE foi: PFE=-8,277+2,146xDBPxCAxCF-2,449xCFxDBP². Os desempenhos de outras fórmulas para estimativa de peso fetal em nossa amostra foram significativamente piores do que os do modelo gerado neste estudo. Equações para predição de percentis condicionais de PFE foram derivadas das avaliações longitudinais do subgrupo IRL-El e validadas com os dados do subgrupo IRL-Val. CONCLUSÕES: descrevemos um método para adaptação de intervalos de referência longitudinais de PFE, sendo este obtido por meio de fórmulas geradas em uma amostra da população brasileira.PURPOSES: To elaborate models for the estimation of fetal weight and longitudinal reference intervals of estimated fetal weight (EFW using a sample of the Brazilian population. METHODS: Prospective observational study. Two groups of patients were evaluated: Group EFW (estimation of

  8. When the entire population is the sample

    DEFF Research Database (Denmark)

    Thygesen, Lau Caspar; Ersbøll, Annette Kjær

    2014-01-01

    -based studies differs from researcher-collected data, all persons in a population are available and traditional statistical analyses focusing on sampling error as the main source of uncertainty may not be relevant. We present the main strengths and limitations of register-based studies, biases especially...

  9. Population Education in Science: Some Sample Lessons.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    This science teacher's manual contains nine sample population education lessons adapted from materials produced in several countries in Asia and Oceania. Activities are designed for lower primary through high school students. Included are class discussions, small group activities, and a role-playing situation. Food chains, human dependence upon…

  10. Population Education in Health: Some Sample Lessons.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    This manual for home economics teachers contains eight sample lessons on health issues related to population growth. Among the topics treated are nutrition, family health, communicable diseases, causes of high mortality, and community health services. Lessons are designed for lower primary through high school students. A scope and sequence chart…

  11. Population Education in Mathematics: Some Sample Lessons.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    This mathematics teacher's manual contains ten sample lessons on population growth and demography that were adapted from materials produced in several countries in Asia and Oceania. Among the mathematics concepts and skills students apply during these lessons are set theory, cardinal and ordinal numbers, frequency tallies, percentages, ratios,…

  12. Estimating Aquatic Insect Populations. Introduction to Sampling.

    Science.gov (United States)

    Chihuahuan Desert Research Inst., Alpine, TX.

    This booklet introduces high school and junior high school students to the major groups of aquatic insects and to population sampling techniques. Chapter 1 consists of a short field guide which can be used to identify five separate orders of aquatic insects: odonata (dragonflies and damselflies); ephemeroptera (mayflies); diptera (true flies);…

  13. TIMP2 gene polymorphism as a potential tool to infer Brazilian population origin

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    da Silva RA

    2013-12-01

    Full Text Available Rodrigo Augusto da Silva,1 André Luis Shinohara,2 Denise Carleto Andia,1 Ariadne Letra,3 Regina Célia Peres,1 Ana Paula de Souza11Department of Morphology, Piracicaba Dental School, State University of Campinas, 2Oral Biology Program, Bauru Dental School, State University of São Paulo, São Paulo, Brazil; 3Department of Endodontics and Center for Craniofacial Research, School of Dentistry, University of Texas Health Science Center, Houston, TX, USAAbstract: Single nucleotide polymorphisms are genome variations that can be used as population-specific markers to infer genetic background and population origin. The Brazilian population is highly admixed due to immigration from several other populations. In particular, the state of São Paulo is recognized for the presence of Japanese individuals who seem likely to have contributed to a substantial proportion of ancestry in the modern Brazilian population. In the present study, we analyzed allele and genotype frequencies and associations of the –418G>C (rs8179090 single nucleotide polymorphism in the TIMP2 gene promoter in Brazilian and Japanese subjects, as well as in Japanese descendants from southeastern Brazil. The allele and genotype frequency analyses among groups demonstrated statistical significance (PC single nucleotide polymorphism of the TIMP2 gene, have a high probability of being Japanese or Japanese descendants. In addition to other genetic polymorphisms, the −418G>C TIMP2 polymorphism could be a population marker to assist in predicting Japanese ancestry, both in Japanese individuals and in admixed populations.Keywords: Brazilian, Japanese, polymorphism, allele, TIMP2

  14. Ideal cardiovascular health prevalence in the Brazilian population - National Health Survey (2013).

    Science.gov (United States)

    Velasquez-Melendez, Gustavo; Felisbino-Mendes, Mariana Santos; Matozinhos, Fernanda Penido; Claro, Rafael; Gomes, Crizian Saar; Malta, Deborah Carvalho

    2015-12-01

    Primordial prevention is defined as the initial prevention of risk factors, through the adoption of healthier behaviors. Within this concept, the American Heart Association (AHA) has defined seven metrics, based on evidence, to achieve ideal cardiovascular health. The aim of this study was to evaluate the prevalence of cardiovascular health in the Brazilian population, according to sex, age, and region of residence, using data from the latest National Health Survey (2013). We assessed the risk factors, as recommended by the AHA, combined (number of factors) and individually: four behavioral (smoking, physical activity, body mass index and diet) and three biological factors (blood pressure, blood glucose and cholesterol levels). The Brazilian population has reached very low prevalence (1%), for the sum of 7 factors in ideal level. Individually, 3.2% of the population consumed ideal diet, followed by physical activity (23.6%) and body mass index (43.7%). The subjects aged between 18 and 35 years showed higher prevalence of metrics combined at the optimal levels (0.5%), which was also reached by the population of the Northern region. These results indicate that greater efforts are urgent by public policies at the level of primordial prevention in order to achieve appropriate targets of cardiovascular health in the Brazilian population.

  15. Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon.

    Science.gov (United States)

    da Costa Francez, Pablo Abdon; Rodrigues, Elzemar Martins Ribeiro; Frazão, Gleycianne Furtado; Dos Reis Borges, Nathalia Danielly; Dos Santos, Sidney Emanuel Batista

    2011-01-01

    The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F(ST) coefficients) to the present database ranged from F(ST) = 0.0016 between Macapá and Belém to F(ST) = 0.0036 between Macapá and the Iberian Peninsula.

  16. Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon

    Science.gov (United States)

    da Costa Francez, Pablo Abdon; Rodrigues, Elzemar Martins Ribeiro; Frazão, Gleycianne Furtado; dos Reis Borges, Nathalia Danielly; dos Santos, Sidney Emanuel Batista

    2011-01-01

    The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African) of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (FST coefficients) to the present database ranged from FST = 0.0016 between Macapá and Belém to FST = 0.0036 between Macapá and the Iberian Peninsula. PMID:21637540

  17. Allelic frequencies and statistical data obtained from 12 codis STR loci in an admixed population of the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Pablo Abdon da Costa Francez

    2011-01-01

    Full Text Available The allelic frequencies of 12 short tandem repeat loci were obtained from a sample of 307 unrelated individuals living in Macapá, a city in the northern Amazon region, Brazil. These loci are the most commonly used in forensics and paternity testing. Based on the allele frequency obtained for the population of Macapá, we estimated an interethnic admixture for the three parental groups (European, Native American and African of, respectively, 46%, 35% and 19%. Comparing these allele frequencies with those of other Brazilian populations and of the Iberian Peninsula population, no significant distances were observed. The interpopulation genetic distances (F ST coefficients to the present database ranged from F ST = 0.0016 between Macapá and Belém to F ST = 0.0036 between Macapá and the Iberian Peninsula.

  18. Distribution of CYP2D6 Alleles and Phenotypes in the Brazilian Population

    Science.gov (United States)

    Sortica, Vinicius A.; Suarez-Kurtz, Guilherme; de Moraes, Maria Elizabete; Pena, Sergio D. J.; dos Santos, Ândrea K. Ribeiro; Romano-Silva, Marco A.; Hutz, Mara H.

    2014-01-01

    Abstract The CYP2D6 enzyme is one of the most important members of the cytochrome P450 superfamily. This enzyme metabolizes approximately 25% of currently prescribed medications. The CYP2D6 gene presents a high allele heterogeneity that determines great inter-individual variation. The aim of this study was to evaluate the variability of CYP2D6 alleles, genotypes and predicted phenotypes in Brazilians. Eleven single nucleotide polymorphisms and CYP2D6 duplications/multiplications were genotyped by TaqMan assays in 1020 individuals from North, Northeast, South, and Southeast Brazil. Eighteen CYP2D6 alleles were identified in the Brazilian population. The CYP2D6*1 and CYP2D6*2 alleles were the most frequent and widely distributed in different geographical regions of Brazil. The highest number of CYPD6 alleles observed was six and the frequency of individuals with more than two copies ranged from 6.3% (in Southern Brazil) to 10.2% (Northern Brazil). The analysis of molecular variance showed that CYP2D6 is homogeneously distributed across different Brazilian regions and most of the differences can be attributed to inter-individual differences. The most frequent predicted metabolic status was EM (83.5%). Overall 2.5% and 3.7% of Brazilians were PMs and UMs respectively. Genomic ancestry proportions differ only in the prevalence of intermediate metabolizers. The IM predicted phenotype is associated with a higher proportion of African ancestry and a lower proportion of European ancestry in Brazilians. PM and UM classes did not vary among regions and/or ancestry proportions therefore unique CYP2D6 testing guidelines for Brazilians are possible and could potentially avoid ineffective or adverse events outcomes due to drug prescriptions. PMID:25329392

  19. Mitochondrial DNA mapping of social-biological interactions in Brazilian Amazonian African-descendant populations

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    Bruno Maia Carvalho

    2008-01-01

    Full Text Available The formation of the Brazilian Amazonian population has historically involved three main ethnic groups, Amerindian, African and European. This has resulted in genetic investigations having been carried out using classical polymorphisms and molecular markers. To better understand the genetic variability and the micro-evolutionary processes acting in human groups in the Brazilian Amazon region we used mitochondrial DNA to investigate 159 maternally unrelated individuals from five Amazonian African-descendant communities. The mitochondrial lineage distribution indicated a contribution of 50.2% from Africans (L0, L1, L2, and L3, 46.6% from Amerindians (haplogroups A, B, C and D and a small European contribution of 1.3%. These results indicated high genetic diversity in the Amerindian and African lineage groups, suggesting that the Brazilian Amazonian African-descendant populations reflect a possible population amalgamation of Amerindian women from different Amazonian indigenous tribes and African women from different geographic regions of Africa who had been brought to Brazil as slaves. The present study partially mapped the historical biological and social interactions that had occurred during the formation and expansion of Amazonian African-descendant communities.

  20. Impact of Physical Activity Interventions on Blood Pressure in Brazilian Populations

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    Vivian Freitas Rezende Bento

    2015-01-01

    Full Text Available Background: High blood pressure is associated with cardiovascular disease, which is the leading cause of mortality in the Brazilian population. Lifestyle changes, including physical activity, are important for lowering blood pressure levels and decreasing the costs associated with outcomes. Objective: Assess the impact of physical activity interventions on blood pressure in Brazilian individuals. Methods: Meta-analysis and systematic review of studies published until May 2014, retrieved from several health sciences databases. Seven studies with 493 participants were included. The analysis included parallel studies of physical activity interventions in adult populations in Brazil with a description of blood pressure (mmHg before and after the intervention in the control and intervention groups. Results: Of 390 retrieved studies, eight matched the proposed inclusion criteria for the systematic review and seven randomized clinical trials were included in the meta-analysis. Physical activity interventions included aerobic and resistance exercises. There was a reduction of -10.09 (95% CI: -18.76 to -1.43 mmHg in the systolic and -7.47 (95% CI: -11.30 to -3.63 mmHg in the diastolic blood pressure. Conclusions: Available evidence on the effects of physical activity on blood pressure in the Brazilian population shows a homogeneous and significant effect at both systolic and diastolic blood pressures. However, the strength of the included studies was low and the methodological quality was also low and/or regular. Larger studies with more rigorous methodology are necessary to build robust evidence.

  1. Evaluation of the Chemical Composition of Brazilian Commercial Cymbopogon citratus (D.C. Stapf Samples

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    Evandro de Castro Melo

    2008-08-01

    Full Text Available Abstract: The concentration and the chemical composition of the essential oils obtained from different samples of Cymbopogon citratus were evaluated. Among the 12 samples investigated (11 dried leaf samples and fresh plant leaves, seven presented essential oil concentrations within the threshold established by the Brazilian legislation. The moisture content was also determined and the majority of the samples presented humidity contents near 12%. The GC and GC/MS analyses of the essential oils led to identification of 22 compounds, with neral and geranial as the two major components. The total percentage of these two compounds varied within the investigated sample oils from 40.7% to 75.4%. In addition, a considerable variation in the chemical composition of the analyzed samples was observed. The process of grinding the leaves significantly decreased (by up to 68% the essential oil content, as well as the percentage of myrcene in the oils.

  2. Cryptic lineages and Pleistocene population expansion in a Brazilian Cerrado frog.

    Science.gov (United States)

    Prado, Cynthia P A; Haddad, Célio F B; Zamudio, Kelly R

    2012-02-01

    Diversification of South American species endemic to open habitats has been attributed to both Tertiary events and Pleistocene climatic fluctuations. Nonetheless, phylogeographical studies of taxa in these regions are few, precluding generalizations about the timing and processes leading to differentiation and speciation. We inferred population structure of Hypsiboas albopunctatus, a frog widely distributed in the Brazilian Cerrado. Three geographically distinct lineages were recovered in our phylogeny. The Chapada dos Guimarães (CG) clade was the first to diverge from other populations and contains multiple haplotypes from a single population in western Cerrado, probably representing a cryptic species. The southeast clade (SE) includes populations along the southeastern limit of the range within the historical distribution of the Brazilian Atlantic forest. Finally, the Central Cerrado (CC) group includes haplotypes from the interior of Brazil that are paraphyletic relative to the SE clade. Analyses of historical demography indicate significant population expansion in the CC and SE populations, likely associated with colonization of newly formed open habitats. The divergence of populations in the CG clade occurred in the late Miocene, concordant with the uplift of the central Brazilian plateau. Divergence of the SE clade from the CC occurred during the mid-Pleistocene. Thus, both Tertiary geological events and Pleistocene climatic fluctuations promoted divergences among lineages. Our study reveals a complex history of diversification in the Cerrado, a morphoclimatic domain highly threatened because of anthropogenic habitat alteration. We identified surprisingly deep divergences in a widely distributed frog, indicating that the Cerrado is not a barrier-free habitat and that its diversity is likely underestimated.

  3. Prevalence and factors associated with latent tuberculosis infection in an indigenous population in the Brazilian Amazon

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    Jocieli Malacarne

    Full Text Available Abstract: INTRODUCTION Recent studies have shown a high incidence and prevalence of latent tuberculosis infection (LTBI in indigenous populations around the World. We aimed to estimate the prevalence and annual risk of infection (ARI as well as to identify factors associated with LTBI in an indigenous population from the Brazilian Amazon. METHODS We conducted a cross-sectional study in 2011. We performed tuberculin skin tests (TSTs, smears and cultures of sputum samples, and chest radiographs for individuals who reported cough for two or more weeks. Associations between LTBI (TST ≥5mm and socio-demographic, clinical, and epidemiological characteristics were investigated using Poisson regression with robust variance. Prevalence ratio (PR was used as the measure of association. RESULTS We examined 263 individuals. The prevalence of LTBI was 40.3%, and the ARI was 2.4%. Age ≥15 years [PR=5.5; 95% confidence interval (CI: 3.5-8.6], contact with tuberculosis (TB patients (PR=3.8; 95% CI: 1.2-11.9, previous TB history (PR=1.4; 95% CI: 1.2-1.7, and presence of Bacillus Calmette-Guérin (BCG scar (PR=1.9, 95% CI: 1.2-2.9 were associated with LTBI. CONCLUSIONS Although some adults may have been infected years prior, the high prevalence of infection and its strong association with age ≥15 years, history of TB, and recent contact with TB patients suggest that the TB transmission risk is high in the study area.

  4. The cognitive subscale of the "Alzheimer's Disease Assessment Scale" in a Brazilian sample

    OpenAIRE

    Schultz R.R.; Siviero M.O.; Bertolucci P.H.F.

    2001-01-01

    The cognitive subscale of the "Alzheimer's Disease Assessment Scale" (ADAS-Cog) is widely used for the evaluation of dementia and is very popular in dementia drug trials because of the characteristics of the scale. The objective of the present study was to adapt the ADAS-Cog for use for the Brazilian population. A major problem is that education is variable, a fact that may influence performance in cognitive evaluation. This study was conducted on a control group (CG) of 96 subjects (25 males...

  5. Dimensions of Oppositionality in a Brazilian Community Sample: Testing the DSM-5 Proposal and Etiological Links

    Science.gov (United States)

    Krieger, Fernanda Valle; Polanczyk, Guilherme Vanoni; Goodman, Robert; Rohde, Luis Augusto; Graeff-Martins, Ana Soledade; Salum, Giovanni; Gadelha, Ary; Pan, Pedro; Stahl, Daniel; Stringaris, Argyris

    2013-01-01

    Objective Investigating dimensions of oppositional symptoms may help to explain heterogeneity of etiology and outcomes for mental disorders across development and provide further empirical justification for the DSM-5–proposed modifications of oppositional defiant disorder (ODD). However, dimensions of oppositionality have not previously been tested in samples outside Europe or the United States. In this study, we used a large Brazilian community sample to compare the fit of different models for dimensions of oppositional symptoms; to examine the association of psychiatric diagnoses and symptoms with dimensions of oppositionality; and to examine the associations between dimensions of oppositionality and parental history of mental disorders. Method A Brazilian community sample of 2,512 children 6 through 12 years old were investigated in this study. Confirmatory factorial analyses were performed to compare the fit of alternative models, followed by linear and logistic regression analyses of associations with psychiatric diagnosis and parental history of psychopathology. Results A three-factor model with irritable, headstrong, and hurtful dimensions fitted best. The irritable dimension showed a strong association with emotional disorders in the child (p<.001) and history of depression (p<.01) and suicidality (p<.05) in the mother. The headstrong dimension was uniquely associated with attention-deficit/hyperactivity disorder (ADHD) in the child (p<.001) and with maternal history of ADHD symptoms (p<.05). The hurtful dimension was specifically associated with conduct disorder (p< .05). Conclusions Our findings from a large community sample of Brazilian children support a distinction between dimensions of oppositionality consistent with current DSM-5 recommendations and provide further evidence for etiological distinctions between these dimensions. PMID:23582870

  6. Mapping the exposure of the Brazilian population to natural background radiation - cosmic radiation

    Energy Technology Data Exchange (ETDEWEB)

    Rochedo, Elaine R.R., E-mail: elaine@ird.gov.br [Instituto de Radioprotecao e Dosimetria (lRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Salles, Krause C.S.; Prado, Nadya M.C., E-mail: krausesalles@yahoo.com.br, E-mail: nadya@ime.ib.br [Instituto Militar de Engenharia (IME), Rio de Janeiro, RJ (Brazil)

    2013-07-01

    The main objective of this work is to statically and graphically describe the exposure of the Brazilian population to natural background radiation. in this stage, doses due to cosmic rays is being assessed based on sea level dose rates, corrected by latitude and altitude, according to the model recommended by UNSCEAR. In this work, the doses were estimated for ali Brazilian municipalities with more than 100.000 inhabitants. The 253 municipalities selected for this study include about 52% of the Brazilian population. Average dose rate was estimated to be about 50 n Sv/h with a variation coefficient of 31%. The estimated doses have shown a strong influence of altitude on dose rates, with a correlation coefficient of 0,998 for ao exponential fit. This result confirms previous studies that show a large effect of the altitude 00 exposure from cosmic radiation. Considering the same occupation and shielding conditions used by UNSCEAR as global averages, average annual dose was estimated to be 0,37 (0,24 - 0,76) mSv/y, very close to UNSCEAR worldwide average of 0,38 (0,3 - 1,0) mSv/y. (author)

  7. Family reintegration of children and adolescents in foster care in Brazilian municipalities with different population sizes.

    Science.gov (United States)

    Iannelli, Andrea M; Assis, Simone Gonçalves; Pinto, Liana Wernersbach; Pinto, Liana Wenersbach

    2015-01-01

    The scope of this article is to present and analyze data from Brazilian foster care services for children / adolescents from the perspective of family reintegration. It also seeks to support the implementation of public policies in order to provide effective reintegration in accordance with the differing local contexts. It uses data from 1,157 municipalities that have foster care services. The methodology takes into account the data collection of 2,624 Brazilian centers and 36,929 children and adolescents in care. The growing number of children/adolescents in care is in line with the increase in population size: 8.4 per small city; 60 per large city and 602.4 per metropolis. With respect to care residence in a different municipality there are varying indices: 12.4% in metropolises and 33.6% in small cities, revealing the absence of centers close to family units in the smaller communities. Regarding the activities promoted together with families, it was seen that there are still units that do not perform any activities, which runs contrary to Brazilian law. It is clear that policies for the child/adolescent in foster care centers need to consider the capacity of the municipality in accordance with population size to implement support actions for families to assist in family reintegration.

  8. Ocelot Population Status in Protected Brazilian Atlantic Forest

    Science.gov (United States)

    Massara, Rodrigo Lima; Paschoal, Ana Maria de Oliveira; Doherty, Paul Francis; Hirsch, André; Chiarello, Adriano Garcia

    2015-01-01

    Forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (Panthera onca) and pumas (Puma concolor), but effects on mesocarnivores, such as ocelots (Leopardus pardalis), are less clear. Ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. We used a standardized camera trap protocol to assess ocelot populations in six protected areas of the Atlantic forest in southeastern Brazil where over 80% of forest remnants are < 50 ha. We tested whether variation in ocelot abundance could be explained by reserve size, forest cover, number of free-ranging domestic dogs and presence of top predators. Ocelot abundance was positively correlated with reserve size and the presence of top predators (jaguar and pumas) and negatively correlated with the number of dogs. We also found higher detection probabilities in less forested areas as compared to larger, intact forests. We suspect that smaller home ranges and higher movement rates in smaller, more degraded areas increased detection. Our data do not support the hypothesis of mesopredator release. Rather, our findings indicate that ocelots respond negatively to habitat loss, and thrive in large protected areas inhabited by top predators. PMID:26560347

  9. Ocelot Population Status in Protected Brazilian Atlantic Forest.

    Science.gov (United States)

    Massara, Rodrigo Lima; Paschoal, Ana Maria de Oliveira; Doherty, Paul Francis; Hirsch, André; Chiarello, Adriano Garcia

    2015-01-01

    Forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (Panthera onca) and pumas (Puma concolor), but effects on mesocarnivores, such as ocelots (Leopardus pardalis), are less clear. Ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. We used a standardized camera trap protocol to assess ocelot populations in six protected areas of the Atlantic forest in southeastern Brazil where over 80% of forest remnants are < 50 ha. We tested whether variation in ocelot abundance could be explained by reserve size, forest cover, number of free-ranging domestic dogs and presence of top predators. Ocelot abundance was positively correlated with reserve size and the presence of top predators (jaguar and pumas) and negatively correlated with the number of dogs. We also found higher detection probabilities in less forested areas as compared to larger, intact forests. We suspect that smaller home ranges and higher movement rates in smaller, more degraded areas increased detection. Our data do not support the hypothesis of mesopredator release. Rather, our findings indicate that ocelots respond negatively to habitat loss, and thrive in large protected areas inhabited by top predators.

  10. Ocelot Population Status in Protected Brazilian Atlantic Forest.

    Directory of Open Access Journals (Sweden)

    Rodrigo Lima Massara

    Full Text Available Forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (Panthera onca and pumas (Puma concolor, but effects on mesocarnivores, such as ocelots (Leopardus pardalis, are less clear. Ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. We used a standardized camera trap protocol to assess ocelot populations in six protected areas of the Atlantic forest in southeastern Brazil where over 80% of forest remnants are < 50 ha. We tested whether variation in ocelot abundance could be explained by reserve size, forest cover, number of free-ranging domestic dogs and presence of top predators. Ocelot abundance was positively correlated with reserve size and the presence of top predators (jaguar and pumas and negatively correlated with the number of dogs. We also found higher detection probabilities in less forested areas as compared to larger, intact forests. We suspect that smaller home ranges and higher movement rates in smaller, more degraded areas increased detection. Our data do not support the hypothesis of mesopredator release. Rather, our findings indicate that ocelots respond negatively to habitat loss, and thrive in large protected areas inhabited by top predators.

  11. Incidência de mutação no códon 12 do protoncogene K-ras em carcinoma de próstata humana em uma amostra da população brasileira The incidence of mutation in codon 12 of the k-ras proto-oncogene in human prostate carcinoma with a Brazilian population sample

    Directory of Open Access Journals (Sweden)

    José Raul Cisternas Gajardo

    2004-06-01

    brazilian population sample analysed demonstrated an intermediary incidence between American and Japanese population. It is unlikely that K-ras mutation isolated has a significant role on the prostatic carcinogenesis in this population.

  12. Sampling and Diversity of Hymenoptera (Insecta) in an Orange Orchard/Brazilian Savannah Fragment Interface

    OpenAIRE

    Rogeria Lara; Daniell Fernandes; Danielle versuti; Maria Tango; Nelson Perioto

    2015-01-01

    The aim of this work was to assess the diversity of Hymenoptera in an orange orchard / Brazilian savannah fragment interface in Descalvado, State of São Paulo, Brazil, using Moericke, Malaise and pitfall traps. The sampling was carried out from February to June 2006, when 5,148 specimens of Hymenoptera, from 12 superfamilies and 36 families, were caught: Chalcidoidea (1,885 specimens; 36.6% out of the total; 14 families), Ichneumonoidea (715; 13.9%; 2), Vespoidea (554; 10.8%; 5), Apoid...

  13. Comparative evaluation of patellar height methods in the Brazilian population

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    Christian Behrendt

    2016-02-01

    Full Text Available ABSTRACT OBJECTIVE: The methods most used for patellar height measurement were compared with the plateau-patella angle method. METHODS: A cross-sectional study was conducted, in which lateral-view radiographs of the knee were evaluated using the three methods already established in the literature: Insall-Salvati (IS, Blackburne-Peel (BP and Caton-Deschamps (CD. These were compared with the plateau-patella angle method. One hundred and ninety-six randomly selected patients were included in the sample. RESULTS: The data were initially evaluated using the chi-square test. This analysis was deemed to be positive with p < 0.0001. We compared the traditional methods with the plateau-patella angle measurement, using Fisher's exact test. In comparing the IS index with the plateau-patella angle, we did not find any statistically significant differences in relation to the proportion of altered cases between the two groups. The traditional methods were compared with the plateau-patella angle with regard to the proportions of cases of high and low patella, by means of Fisher's exact test. This analysis showed that the plateau-patella angle identified fewer cases of high patella than did the IS, BP and CD methods, but more cases of low patella. In comparing pairs, we found that the IS and CD indices were capable of identifying more cases of high patella than was the plateau-patella angle. In relation to the cases of low patella, the plateau-patella angle was capable of identifying more cases than were the other three methods. CONCLUSIONS: The plateau-patella angle found more patients with low patella than did the classical methods and showed results that diverged from those of the other indices studied.

  14. New Brazilian giant viruses isolation from environmental samples using a panel of protozoa.

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    Fábio Pio Dornas

    2015-10-01

    Full Text Available The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses, we propose the use of different species of amoeba as a cellular support. The aim of this work was to isolate new Brazilian giant viruses by comparing the protozoa Acanthamoeba castellanii, A. polyphaga, A. griffini and Vermamoeba vermiformis as a platform for cellular isolation using environmental samples. One hundred samples were collected from 3 different areas in September 2014 in the Pampulha lagoon of Belo Horizonte city, Minas Gerais, Brazil. PCR was used to identify the isolated viruses, along with hemacolor staining, labelling fluorescence and electron microscopy. A total of 69 viruses were isolated. The highest ratio of isolation was found in Acanthamoeba polyphaga (46.38% and the lowest in Vermamoeba vermiformis (0%. Mimiviruses were the most frequently isolated. One Marseillevirus and one Pandoravirus were also isolated. With Brazilian environmental samples, we demonstrated the high rate of lineage A mimiviruses. This work demonstrates how these viruses survive and circulate in nature as well the differences between protozoa as a platform for cellular isolation.

  15. Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa

    Science.gov (United States)

    Dornas, Fábio P.; Khalil, Jacques Y. B.; Pagnier, Isabelle; Raoult, Didier; Abrahão, Jônatas; La Scola, Bernard

    2015-01-01

    The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses, we propose the use of different species of amoeba as a cellular support. The aim of this work was to isolate new Brazilian giant viruses by comparing the protozoa Acanthamoeba castellanii, A. polyphaga, A. griffini, and Vermamoeba vermiformis (VV) as a platform for cellular isolation using environmental samples. One hundred samples were collected from 3 different areas in September 2014 in the Pampulha lagoon of Belo Horizonte city, Minas Gerais, Brazil. PCR was used to identify the isolated viruses, along with hemacolor staining, labelling fluorescence and electron microscopy. A total of 69 viruses were isolated. The highest ratio of isolation was found in A. polyphaga (46.38%) and the lowest in VV (0%). Mimiviruses were the most frequently isolated. One Marseillevirus and one Pandoravirus were also isolated. With Brazilian environmental samples, we demonstrated the high rate of lineage A mimiviruses. This work demonstrates how these viruses survive and circulate in nature as well the differences between protozoa as a platform for cellular isolation. PMID:26500630

  16. Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae populations from southern Brazilian Atlantic rainforest remnants

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    Silvia H. Sofia

    2005-09-01

    Full Text Available Random amplified polymorphic DNA (RAPD markers were used to analyze the genetic structure of Eufriesea violacea populations in three fragments (85.47, 832.58 and 2800 ha of Atlantic rainforest located in the north of the Brazilian state of Paraná. A total of twelve primers produced 206 loci, of which 129 were polymorphic (95% criterion. The proportions of polymorphic loci in each population ranged from 57.28% to 59.2%, revealing very similar levels of genetic variability in the groups of bees from each fragment. Unbiased genetic distances between groups ranged from 0.0171 to 0.0284, the smallest genetic distance occurring between bees from the two larger fragments. These results suggest that the E. violacea populations from the three fragments have maintained themselves genetically similar to native populations of this species originally present in northern Paraná.

  17. Translating Population Difference: The Use and Re-Use of Genetic Ancestry in Brazilian Cancer Genetics.

    Science.gov (United States)

    Gibbon, Sahra

    2016-01-01

    In the past ten years, there has been an expansion of scientific interest in population genetics linked to both understanding histories of human migration and the way that population difference and diversity may account for and/or be implicated in health and disease. In this article, I examine how particular aspects of a globalizing research agenda related to population differences and genetic ancestry are taken up in locally variant ways in the nascent field of Brazilian cancer genetics. Drawing on a broad range of ethnographic data from clinical and nonclinical contexts in the south of Brazil, I examine the ambiguities that attention to genetic ancestry generates, so revealing the disjunctured and diverse ways a global research agenda increasingly orientated to questions of population difference and genetic ancestry is being used and reused.

  18. [Live longer and better? Estimates of healthy life expectancy in the Brazilian population].

    Science.gov (United States)

    Camargos, Mirela Castro Santos; Gonzaga, Marcos Roberto

    2015-07-01

    This study analyzed differences in healthy life expectancy in the elderly based on three health dimensions in Brazil from 1998 to 2008: disability-free life expectancy, healthy life expectancy based on self-rated health, and chronic disease-free life expectancy. The Sullivan method was used, combining life tables from the Brazilian Institute of Geography and Statistics (IBGE) and interval estimates of the prevalence of functional disability, self-rated health, and chronic diseases according to the Brazilian National Household Sample Survey (PNAD, 1998 and 2008). Besides the increase in life expectancy, the study showed significant and similar increases in disability-free life expectancy and healthy life expectancy based on self-rated health at almost all ages. Women had higher life expectancies than men, but expected to live longer with poor health, regardless of the indicator used to measure health. Although the studies measured health differently (making comparisons difficult), women showed a consistent disadvantage in healthy life expectancy.

  19. Cannabinoid Type-1 Receptor Gene Polymorphisms Are Associated with Central Obesity in a Southern Brazilian Population

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    Janaína P. Jaeger

    2008-01-01

    Full Text Available The CB1 cannabinoid receptor and its endogenous ligands, the endocannabinoids, are involved in energy balance control, stimulating appetite and increasing body weight in wasting syndromes. Different studies have investigated the relationship between polymorphisms of the cannabinoid receptor 1 (CNR1 gene and obesity with conflicting results. In the present study, we investigated the 1359G/A (rs1049353, 3813A/G (rs12720071 and 4895A/G (rs806368 polymorphisms in the CNR1 gene in a Brazilian population of European descent. To verify the association between these variants and obesity-related traits in this population, 756 individuals were genotyped by PCR-RFLP methods. The 4895G allele was associated with waist to hip ratio (WHR (P = 0.014; P = 0.042 after Bonferroni correction. An additive effect with the GAA haplotype was associated with WHR (P = 0.028, although this statistical significance disappeared after Bonferroni correction (P = 0.084. No significant association was observed between the genotypes of the 1359G/A and 3813A/G polymorphisms and any of the quantitative variables investigated. Our findings suggest that CNR1 gene polymorphism is associated with central obesity in this Brazilian population of European ancestry.

  20. The Sample Complexity of Search over Multiple Populations

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    Malloy, Matthew L.; Tang, Gongguo; Nowak, Robert D.

    2012-01-01

    This paper studies the sample complexity of searching over multiple populations. We consider a large number of populations, each corresponding to either distribution P0 or P1. The goal of the search problem studied here is to find one population corresponding to distribution P1 with as few samples as possible. The main contribution is to quantify the number of samples needed to correctly find one such population. We consider two general approaches: non-adaptive sampling methods, which sample ...

  1. Association study between the Taq1A (rs1800497 polymorphism and schizophrenia in a Brazilian sample

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    Quirino Cordeiro

    2014-08-01

    Full Text Available Schizophrenia is a severe psychotic disorder with recurrent relapse and functional impairment. It results from a poorly understood gene-environment interaction. The Taq1A polymorphism (located in the gene cluster NTAD is a likely candidate for schizophrenia. Its rs1800497 polymorphism was shown to be associated with DRD2 gene expression. Therefore the present work aims to investigate a possible association between schizophrenia and such polymorphism. The compared distribution of the alleles and genotypes of the studied polymorphism was investigated in a Brazilian sample of 235 patients and 834 controls. Genotypic frequencies were in Hardy-Weinberg equilibrium. There was a trend of allelic association between the Taq1A polymorphism (rs1800497 with schizophrenia in the studied sample. However no statistically differences were found between cases and controls when analyzed by gender or schizophrenia subtypes.

  2. Philadelphia Brief Assessment of Cognition in healthy and clinical Brazilian sample

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    Danilo Assis Pereira

    2012-03-01

    Full Text Available The Philadelphia Brief Assessment of Cognition (PBAC is a neuropsychological screening instrument that assesses five cognitive domains: working memory, visuospatial functioning, language, episodic memory and comportment. The aim is to verify if PBAC can properly be used in the Brazilian sample. Participated in this study: (a 200 healthy volunteers - 100 young [21.6(2.5 years old] and 100 older adults [70.1(7.3 years old]; >12 years of education; (b 30 Alzheimer's patients (AD [73.7(5.7 years old], 4-11 years in education. The PBAC scores: (a 95.8(2.6, 90.0(4.4 and (b 65.0(10.8 were correlated with the Mini-Mental State Examination (MMSE for young 29.1(0.9, older adults 28.3(1.4 and AD 18.4(3.0 groups. A positive correlation between MMSE and PBAC (r=0.9, p<0.001 was found. Negative correlations were observed between PBAC domains [memory (-0.63, visuospatial abilities (-0.44 and working memory (-0.3 tasks]. MANOVA showed a better male performance in visuospatial functioning (F=8.5, p=0.004. The Brazilian version of PBAC proved to be a promising screening instrument for clinical purposes.

  3. Reference serving sizes for the Brazilian population: An analysis of processed food labels

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    Nathalie Kliemann

    2014-06-01

    Full Text Available OBJECTIVE: To compare serving sizes reported on processed food labels with reference serving sizes according to nutrition labeling legislation and the "Food Guide for the Brazilian Population". METHODS: This cross-sectional study analyzed the labels of 2,072 processed foods in a supermarket of Florianópolis, Santa Caratina, Brazil. The foods were classified according to the Brazilian food labeling legislation. Central tendency and variability values were calculated for the serving sizes and energy values reported on the labels, as well as the ratio between the reported and reference energy value. The Spearman correlation test was performed between the reference serving size and the reference energy density, and also between the reference serving size and energy density of each study food. RESULTS: Nutrition labeling and the Food Guide presented reference servings with different sizes and energy values. The serving sizes reported on the labels did not follow either of the references and presented heterogeneous values, with a maximum range of 55-240 g among ready and semi-ready pre-prepared dishes. The reported energy values were between 0.1 times smaller and 2.4 times larger than the reference values. The reference serving sizes presented a highly inverse correlation with the reference energy density (Spearman coefficient= 0.9 and a very low inverse correlation with the energy density of the foods analyzed (Spearman coefficient= 0.2. CONCLUSION: This study showed the need for standardizing reference serving size information for the Brazilian population as well as reviewing nutrition labeling legislation in order to standardize the serving sizes reported on labels and to update the reference energy density used to calculate serving sizes.

  4. Flavonoids in vegetable foods commonly consumed in Brazil and estimated ingestion by the Brazilian population.

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    Arabbi, Paola R; Genovese, Maria Inés; Lajolo, Franco M

    2004-03-10

    The objective of this work was to quantify the flavonoids present in foods most commonly consumed by the Brazilian population. The predominant flavonoids found in largest abundance in all of the analyzed vegetables were glycosides of quercetin. In lettuce, a small amount of luteolin was also detected. In sweet pepper, quercetin and luteolin were both present. White onion [48-56 mg/100 g of fresh weight (FW), expressed as aglycon], red onion (40-100 mg/100 g of FW), red lettuce (67-67.2 mg/100 g of FW), arugula (41-118 mg/100 g of FW), and chicory (18-38 mg/100 g of FW) were highest in total flavonoids. In fruits, the highest concentrations of flavonoids were found in the peel (125-170 mg/100 g of FW) and pulp (35-44 mg/100 g of FW) of oranges and in some apple varieties (14-36 mg/100 g of FW). Variability in flavonoid content due to time of harvesting was high for leafy vegetables and red onions. The estimated ingestion by Brazilian population ranged from 60 to 106 mg/day.

  5. Association of leprosy with HLA-DR2 in a Southern Brazilian population

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    Visentainer J.E.L.

    1997-01-01

    Full Text Available The association between HLA specificities and leprosy was investigated in a Southern Brazilian population. One hundred and twenty-one patients and 147 controls were typed for HLA-A, B, Cw, DR and DQ. Patients were subdivided into the following subgroups, according to clinical, histological and immunological criteria: lepromatous (N = 55, tuberculoid (N = 32, dimorphous (N = 20, and indeterminate (N = 14. The frequencies of HLA specificities were compared between the total group of patients and controls, and between the same controls and each subgroup of patients. After correction of the probabilities, deviations were not significant, except for the DR2 specificity, which presented a frequency of 44.2% in the total group of patients and 56.3% in the subgroup of individuals with the tuberculoid form of the disease, compared to 23.3% in the controls. Stratified analysis showed that the increased DR2 frequency in the total group of patients was due to the subgroups with the tuberculoid and dimorphous forms. The relative risk of tuberculoid leprosy for DR2-positive individuals was 4.2, and the etiologic fraction of DR2 was 0.429. In conclusion, a positive association of the DR2 specificity with the tuberculoid form of leprosy, but not with the lepromatous, dimorphous, or indeterminate forms, was demonstrated in this Southern Brazilian population

  6. Brazilian samples of bee samples pollen: palynological origin, phenolic content, antioxidant properties and antimicrobial activity

    OpenAIRE

    Arruda, Vanilda Aparecida Soares de; Santos, Alexandre Vieira dos; Figueiredo, Davi; Meira, Sampaio; Leticia M. Estevinho; Barth, Ortrud Monika; Freitas, Alex da Silva; Almeida-Muradian,Ligia Bicudo de

    2012-01-01

    Total phenolic and flavonoids phytochemical concentration was measured in bee pollen samples, Appis Mellifera , as well as their botanical origin, antioxidant activity and antimicrobial activity. Pollen loads were washed with 70% ethanol and identified using a 400x magnification. The content of total polyphenols and flavonoids was measured spectrophotometrically with gallic acid and quercetin as standard. The antioxidant activity was evaluated by the linking capacity of (i)the fre...

  7. Global pharmacogenomics: distribution of CYP3A5 polymorphisms and phenotypes in the Brazilian population.

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    Guilherme Suarez-Kurtz

    Full Text Available The influence of self-reported "race/color", geographical origin and genetic ancestry on the distribution of three functional CYP3A5 polymorphisms, their imputed haplotypes and inferred phenotypes was examined in 909 healthy, adult Brazilians, self-identified as White, Brown or Black ("race/color" categories of the Brazilian census. The cohort was genotyped for CYP3A5*3 (rs776746, CYP3A5*6 (rs10264272 and CYP3A5*7 (rs41303343, CYP3A5 haplotypes were imputed and CYP3A5 metabolizer phenotypes were inferred according to the number of defective CYP3A5 alleles. Estimates of the individual proportions of Amerindian, African and European ancestry were available for the entire cohort. Multinomial log-linear regression models were applied to infer the statistical association between the distribution of CYP3A5 alleles, haplotypes and phenotypes (response variables, and self-reported Color, geographical region and ancestry (explanatory variables. We found that Color per se or in combination with geographical region associates significantly with the distribution of CYP3A5 variant alleles and CYP3A5 metabolizer phenotypes, whereas geographical region per se influences the frequency distribution of CYP3A5 variant alleles. The odds of having the default CYP3A5*3 allele and the poor metabolizer phenotype increases continuously with the increase of European ancestry and decrease of African ancestry. The opposite trend is observed in relation to CYP3A5*6, CYP3A5*7, the default CYP3A5*1 allele, and both the extensive and intermediate phenotypes. No significant effect of Amerindian ancestry on the distribution of CYP3A5 alleles or phenotypes was observed. In conclusion, this study strongly supports the notion that the intrinsic heterogeneity of the Brazilian population must be acknowledged in the design and interpretation of pharmacogenomic studies, and dealt with as a continuous variable, rather than proportioned in arbitrary categories that do not capture the

  8. Polymorphisms of the DNA repair genes XRCC1 and XRCC3 in a Brazilian population

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    Duarte Márcia Cristina

    2005-01-01

    Full Text Available In several DNA repair genes, polymorphisms may result in reduced repair capacity, which has been implicated as a risk factor for various types of cancer. The frequency of the polymorphic alleles varies among populations, suggesting an ethnic distribution of genotypes. We genotyped 300 healthy Southeastern Brazilian individuals (262 of European ancestry and 38 of African ancestry for polymorphisms of codons 194 and 399 of the XRCC1 base excision repair pathway gene and of codon 241 of the XRCC3 homologous recombination repair pathway gene. The allele frequencies were 0.07 for the Arg194Trp and 0.33 for the Arg399Gln codons of the XRCC1 gene and 0.35 for the Thr241Met codon of the XRCC3 gene. The genotypic frequencies were within Hardy-Weinberg equilibrium. These frequencies showed ethnic variability when compared with those obtained for different populations from several countries.

  9. Relationships Between Positive and Negative Affect and the Five Factors of Personality in a Brazilian Sample

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    Cristian Zanon

    2013-09-01

    Full Text Available Strong associations of Neuroticism and Extraversion with positive affects (PA and negative affects (NA have been reported in the international literature. This study aimed to evaluate the occurrence of such relationships in a Brazilian sample, and also to investigate the role of Conscientiousness, Agreeableness and Openness in the prediction of PA and NA through the use of a hybrid structural model. Participants were 319 university students, between 17 and 37 years of age (mean = 21.5, SD = 4.9. Approximately 64% of the students were female and 36% male. Results showed that Neuroticism was the most important predictor of PA and NA, followed by Conscientiousness, but not Extraversion. Surprisingly, Agreeableness was shown to be a weak prediction for NA, but had no relationship with PA. As expected, Openness showed no relationship with PA or NA. These results are partially in agreement with the international literature but some important differences were detected.

  10. Modelagem do crescimento populacional do rebanho bovino brasileiro Modeling the growth of Brazilian cattle population

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    Alfredo Ribeiro de Freitas

    2005-12-01

    Full Text Available Considerando-se o número efetivo de animais e a taxa de abate do rebanho bovino brasileiro no período de 1983 a 2000, estimou-se o crescimento dessa população utilizando-se o modelo de Richards, ajustado pela técnica de verossimilhança profile. O modelo se mostrou adequado para descrever o crescimento da população brasileira de bovinos, pois as superestimavas e ou subestimavas dos valores se situaram entre 1 e 2,5%. A partir da modelagem por cadeia de Markov, foram calculados a probabilidade de o rebanho atingir 200 milhões de animais até o ano de 2015, em função da taxa de abate, e o tempo esperado para se atingir este tamanho populacional, em função da taxa de abate. A probabilidade de o rebanho atingir 200 milhões de animais até o ano de 2015, a uma taxa de abate de aproximadamente 17%, é 0,7. Com taxa de abate anual de 16%, o rebanho atingirá esse tamanho no período de 11 anos e, com taxa de abate de 18%, em 20 anos.The growth of the Brazilian bovine cattle population was evaluated using the effective number of animals and the annual slaughter rate from 1983 to 2000. The Richards model was fitted with the profile likelihood technique. Two population parameters were calculated by Markov chain modeling: a the probability of the cattle population to reach 200 million of animals in 2015 as a function of the slaughter rate; b the time to reach this size, considering different annual slaughter rates. The Richards model was adequate to estimate the Brazilian cattle population growth since overestimated and/or underestimated values ranged between one and 2.5%. The probability of the Brazilian herd to reach 200 millions animals in 2015 for an annual slaughter rate of approximately 17% is 0.7 and the expected time to reach 200 million animals for annual slaughter rates of 16% and 18% was 11 and 20 years respectively.

  11. Removal of nitrogen compounds from Brazilian petroleum samples by oxidation followed by liquid-liquid extraction

    Energy Technology Data Exchange (ETDEWEB)

    Conceicao, L.; Pergher, S.B.C. [Universidade Regional Integrada do Alto Uruguai e das Misses (URI), Erechim, RS (Brazil). Dept. de Quimica], E-mail: pergher@uricer.edu.br; Oliveira, J.V. [Universidade Regional Integrada do Alto Uruguai e das Misses (URI), Erechim, RS (Brazil). Dept. de Engenharia dos Alimentos; Souza, W.F. [Petroleo Brasileiro S.A. (CENPES/PETROBRAS), Rio de Janeiro, RJ (Brazil). Centro de Pesquisas

    2009-10-15

    This work reports liquid-liquid extraction of nitrogen compounds from oxidized and non-oxidized Brazilian petroleum samples. The experiments were accomplished in a laboratory-scale liquid-liquid apparatus in the temperature range of 303 K-323 K, using methanol, n-methyl-2-pyrrolidone (NMP) and N,Ndimethylformamide (DMF), and their mixtures as extraction solvents, employing solvent to sample volume ratios of 1:2, 1:1 and 2:1, exploring up to three separation stages. Results show that an increase in temperature, solvent to oil ratio, and number of equilibrium stages greatly improves the nitrogen removal from the oxidized sample (from 2600 to 200 ppm). The employed oxidation scheme is thus demonstrated to be an essential and efficient step of sample preparation for the selective liquid-liquid removal of nitrogen compounds. It is shown that the use of mixtures of DMF and NMP as well their use as co-solvents with methanol did not prove to be useful for selective nitrogen extraction since great oil losses were observed in the final process. (author)

  12. Role of ACE and AGT gene polymorphisms in genetic susceptibility to diabetes mellitus type 2 in a Brazilian sample.

    Science.gov (United States)

    Wollinger, L M; Dal Bosco, S M; Rempe, C; Almeida, S E M; Berlese, D B; Castoldi, R P; Arndt, M E; Contini, V; Genro, J P

    2015-12-29

    The aim of the current study was to investigate the association between the InDel polymorphism in the angiotensin I-converting enzyme gene (ACE) and the rs699 polymorphism in the angiotensinogen gene (AGT) and diabetes mellitus type 2 (DM2) in a sample population from Southern Brazil. A case-control study was conducted with 228 patients with DM2 and 183 controls without DM2. The ACE InDel polymorphism was genotyped by polymerase chain reaction (PCR) with specific primers, followed by electrophoresis on 1.5% agarose gel. The AGT rs699 polymorphism was genotyped using a real-time PCR assay. No significant association between the ACE InDel polymorphism and DM2 was detected (P = 0.97). However, regarding the AGT rs699 polymorphism, DM2 patients had a significantly higher frequency of the AG genotype and lower frequency of the GG genotype when compared to the controls (P = 0.03). Our results suggest that there is an association between the AGT rs699 polymorphism and DM2 in a Brazilian sample.

  13. Comparative evaluation of two populations of Pseudophilothrips ichini as candidates for biological control of Brazilian peppertree

    Science.gov (United States)

    Brazilian peppertree, Schinus terebinthifolius Raddi (Sapindales: Anacardiaceae) is one of the worst invasive species in Florida. The thrips Pseudophilothrips ichini Hood (Thysanoptera: Phlaeothripidae) is being considered as a potential biological control agent of Brazilian peppertree. Two populati...

  14. Investigation of host candidate malaria-associated risk/protective SNPs in a Brazilian Amazonian population.

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    Simone da Silva Santos

    Full Text Available The Brazilian Amazon is a hypo-endemic malaria region with nearly 300,000 cases each year. A variety of genetic polymorphisms, particularly in erythrocyte receptors and immune response related genes, have been described to be associated with susceptibility and resistance to malaria. In order to identify polymorphisms that might be associated with malaria clinical outcomes in a Brazilian Amazonian population, sixty-four human single nucleotide polymorphisms in 37 genes were analyzed using a Sequenom massARRAY iPLEX platform. A total of 648 individuals from two malaria endemic areas were studied, including 535 malaria cases (113 individuals with clinical mild malaria, 122 individuals with asymptomatic infection and 300 individuals with history of previous mild malaria and 113 health controls with no history of malaria. The data revealed significant associations (p<0.003 between one SNP in the IL10 gene (rs1800896 and one SNP in the TLR4 gene (rs4986790 with reduced risk for clinical malaria, one SNP in the IRF1 gene (rs2706384 with increased risk for clinical malaria, one SNP in the LTA gene (rs909253 with protection from clinical malaria and one SNP in the TNF gene (RS1800750 associated with susceptibility to clinical malaria. Also, a new association was found between a SNP in the CTL4 gene (rs2242665, located at the major histocompatibility complex III region, and reduced risk for clinical malaria. This study represents the first association study from an Amazonian population involving a large number of host genetic polymorphisms with susceptibility or resistance to Plasmodium infection and malaria outcomes. Further studies should include a larger number of individuals, refined parameters and a fine-scale map obtained through DNA sequencing to increase the knowledge of the Amazonian population genetic diversity.

  15. Use of generic medicines by the Brazilian population: an evaluation of PNAUM 2014

    Science.gov (United States)

    Bertoldi, Andréa Dâmaso; Arrais, Paulo Sergio Dourado; Tavares, Noemia Urruth Leão; Ramos, Luiz Roberto; Luiza, Vera Lucia; Mengue, Sotero Serrate; Dal-Pizzol, Tatiane da Silva; Farias, Mareni Rocha; Oliveira, Maria Auxiliadora

    2016-01-01

    ABSTRACT OBJECTIVE To analyze the existence of differences in the use of generic medicines in Brazil according to demographic and socioeconomic variables and acquisition sources of the medicines. METHODS Population-based cross-sectional study, conducted with data from the Pesquisa Nacional de Acesso, Utilização e Promoção do Uso Racional de Medicamentos (PNAUM – National Survey on Access, Use and Promotion of Rational Use of Medicines). Data collection took place between September, 2013 and February, 2014 in homes of Brazilian cities (urban area). The use of medicines has been investigated in relation to the treatment of chronic diseases and, in the case of acute events, regarding use over the previous 15 days. Generics were identified by visualization of packaging presented by the users of the medicines. The independent variables used were sex, age, education level, economic class, and region of the Country. The statistical significance of differences between the groups was evaluated by Pearson’s Chi-squared test, considering a 5% significance level. RESULTS The prevalence of generic medicines use was 45.5% (95%CI 43.7–47.3). There was no difference considering education level. The prevalence was higher in females (47.0%; 95%CI 44.9–49.0) than in males (43.1%; 95%CI 40.5–45.8), and were higher with increasing age. Generic medicines were more used in the economic class C (47.0%; 95%CI 44.9–49.1) and in the South (50.6%; 95%CI 46.6–54.6) and Southeast (49.9%; 95%CI 46.8–53.0) regions. Generics accounted for 37.3% of the medicines provided by the Brazilian Unified Health System. CONCLUSIONS Currently, there is a choice of purchase or free provision by the Brazilian Unified Health System, characterized by quality assurance and reduced price regarding branded medicines considered as reference. In the private market, a considerable part of the population is choosing generic medicines thanks to the availability of this option for virtually all

  16. Monitoring light hydrocarbons in Brazilian coal mines and in confined coal samples

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Rosangela [Pos-Graduation Program in Materials Engineering and Technology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre (Brazil); Pires, Marcal [Pos-Graduation Program in Materials Engineering and Technology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre (Brazil); Faculty of Chemistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre (Brazil); Azevedo, Carla M.N. [Faculty of Chemistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre (Brazil); Fagundes, Leandro [P and D Consultoria (Brazil); Garavaglia, Luciane; Gomes, Cleber J.B. [SATC, Associacao Beneficente da Industria Carbonifera de Santa Catarina, CTCL, Technological Center of Clean Coal (Brazil)

    2010-12-01

    Monitoring light hydrocarbons (LHCs) in coal mines, particularly methane, is important not only because of their implications for global climate change but also for economic and safety reasons. Furthermore, the identification and quantification of LHCs in coal mine air samples and desorbed from confined coal may contribute to a better understanding of coal seam characteristics. The paucity of information about the levels of methane in Brazilian underground coal mines can be attributed to their difficult access and a lack of adequate procedures for correct gas sampling. The aim of this study is to optimize and apply standard gas chromatography procedures to determine LHC levels in the air of coal mines and in confined coal from five mines under operation, three underground (A, B, C) and two surface (D, E) mines, in southern Brazil. The results indicate methane (C{sub 1}) levels varying from 3 ppm to 27% in the atmosphere of the underground mines. Mine A presented high levels of all LHCs analyzed (C{sub 1} to C{sub 5}), while only C{sub 1} and C{sub 2} were detected in mine B, and hydrocarbons ranging from C{sub 1} to C{sub 4} were found in mine C. On the other hand, surface mines presented narrow concentration range for C{sub 1} (3 ppm to 470 ppm) and C{sub 2}-C{sub 3}, with higher levels observed for puncture explosive points. Among LHCs, methane is desorbed in higher concentrations from confined coals and the presence of C{sub 2} was detected in all samples while C{sub 3}-C{sub 5} were only observed in coals from underground mines. These data are consistent with those obtained from the air gas samples collected in the mines under study. Geological events such as faulting and intrusions can accelerate the release of gas or the trapping of large amounts of previously released methane. The LHC emissions from coal mines were found to be highly variable, indicating the need for a comprehensive survey of Brazilian coal mine emissions. (author)

  17. QTL mapping for yield components and agronomic traits in a Brazilian soybean population

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    Josiane Isabela da Silva Rodrigues

    2016-11-01

    Full Text Available The objective of this work was to map QTL for agronomic traits in a Brazilian soybean population. For this, 207 F2:3 progenies from the cross CS3035PTA276-1-5-2x UFVS2012 were genotyped and cultivated in Viçosa-MG, using randomized block design with three replications. QTL detection was carried out by linear regression and composite interval mapping. Thirty molecular markers linked to QTL were detected by linear regression for the total of nine agronomic traits. QTL for SWP (seed weight per plant, W100S (weight of 100 seeds, NPP (number of pods per plant, and NSP (number of seeds per plant were detected by composite interval mapping. Four QTL with additive effect are promising for marker-assisted selection (MAS. Particularly, the markers Satt155 and Satt300 could be useful in simultaneous selection for greater SWP, NPP, and NSP.

  18. Unraveling Brazilian Indian population prostate good health: clinical, anthropometric and genetic features

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    Mario M. de Lima Junior

    2015-04-01

    Full Text Available Purpose To compare dietary, lifestyle, clinical, anthropometric, genetic and prostatic features of Brazilian Indians and non-Indians (Amazon. Methods 315 men, 228 Indians and 89 non-Indians, ≥40 years old were submitted to digital rectal examination, serum prostate specific antigen (PSA, testosterone, TP53 and GSTP1 genotyping, anthropometric, lifestyle, dietary, personal and familial medical history. Prostatic symptoms were evaluated with the International Prostate Symptom Score (IPSS. Results Macuxis and Yanomamis represented 43.6% and 14.5% of Indians respectively who spontaneously referred no prostate symptoms. Mean IPSS was 7, range 3-19, with only 15% of moderate symptoms (score 8-19; Mean age was 54.7 years, waist circumference 86.6 cm, BMI 23.9 kg/m2. Yanomamis presented both lower BMI (21.4 versus 24.8 and 23.3, p=0,001 and prostate volume than Macuxis and “other ethnic groups” (15 versus 20, p=0.001. Testosterone (414 versus 502 and 512, p=0.207 and PSA (0.48 versus 0.6 and 0.41, p=0.349 were similar with progressive PSA increase with aging. Val/Val correlated with lower PSA (p=0.0361. Indians compared to control population presented: - TP53 super representation of Arg/Arg haplotype, 74.5% versus 42.5%, p<0.0001. -GSTP1 Ile/Ile 35.3% versus 60.9%; Ile/Val 45.9% versus 28.7%; Val/Val 18.8% versus 10.3%; p=0.0003. Conclusions Observed specific dietary, lifestyle, anthropometric and genetic profile for TP53 and GSTP1 may contribute to Brazilian Indian population prostate good health.

  19. Several different lactase persistence associated alleles and high diversity of the lactase gene in the admixed Brazilian population.

    Science.gov (United States)

    Friedrich, Deise C; Santos, Sidney E B; Ribeiro-dos-Santos, Ândrea K C; Hutz, Mara H

    2012-01-01

    Adult-type hypolactasia is a common phenotype caused by the lactase enzyme deficiency. The -13910 C>T polymorphism, located 14 Kb upstream of the lactase gene (LCT) in the MCM6 gene was associated with lactase persistence (LP) in Europeans. This polymorphism is rare in Africa but several other variants associated with lactase persistence were observed in Africans. The aims of this study were to identify polymorphisms in the MCM6 region associated with the lactase persistence phenotype and to determine the distribution of LCT gene haplotypes in 981 individuals from North, Northeast and South Brazil. These polymorphisms were genotyped by PCR based methods and sequencing. The -13779*C,-13910*T, -13937*A, -14010*C, -14011*T LP alleles previously described in the MCM6 gene region that acts as an enhancer for the LCT gene were identified in Brazilians. The most common LP allele was -13910*T. Its frequency was highly correlated with European ancestry in the Brazilian populations investigated. The -13910*T was higher (0.295) in southern Brazilians of European ancestry and lower (0.175) in the Northern admixed population. LCT haplotypes were derived from the 10 LCT SNPs genotyped. Overall twenty six haplotypes previously described were identified in the four Brazilian populations studied. The Multidimensional Scaling analysis showed that Belém, in the north, was closer to Amerindians. Northeastern and southern Afro-descendants were more related with Bantu-speaking South Africans whereas the Southern population with European ancestry grouped with Southern and Northern Europeans. This study shows a high variability considering the number of LCT haplotypes observed. Due to the highly admixed nature of the Brazilian populations, the diagnosis of hypolactasia in Brazil, based only in the investigation of the -13910*T allele is an oversimplification.

  20. Several different lactase persistence associated alleles and high diversity of the lactase gene in the admixed Brazilian population.

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    Deise C Friedrich

    Full Text Available Adult-type hypolactasia is a common phenotype caused by the lactase enzyme deficiency. The -13910 C>T polymorphism, located 14 Kb upstream of the lactase gene (LCT in the MCM6 gene was associated with lactase persistence (LP in Europeans. This polymorphism is rare in Africa but several other variants associated with lactase persistence were observed in Africans. The aims of this study were to identify polymorphisms in the MCM6 region associated with the lactase persistence phenotype and to determine the distribution of LCT gene haplotypes in 981 individuals from North, Northeast and South Brazil. These polymorphisms were genotyped by PCR based methods and sequencing. The -13779*C,-13910*T, -13937*A, -14010*C, -14011*T LP alleles previously described in the MCM6 gene region that acts as an enhancer for the LCT gene were identified in Brazilians. The most common LP allele was -13910*T. Its frequency was highly correlated with European ancestry in the Brazilian populations investigated. The -13910*T was higher (0.295 in southern Brazilians of European ancestry and lower (0.175 in the Northern admixed population. LCT haplotypes were derived from the 10 LCT SNPs genotyped. Overall twenty six haplotypes previously described were identified in the four Brazilian populations studied. The Multidimensional Scaling analysis showed that Belém, in the north, was closer to Amerindians. Northeastern and southern Afro-descendants were more related with Bantu-speaking South Africans whereas the Southern population with European ancestry grouped with Southern and Northern Europeans. This study shows a high variability considering the number of LCT haplotypes observed. Due to the highly admixed nature of the Brazilian populations, the diagnosis of hypolactasia in Brazil, based only in the investigation of the -13910*T allele is an oversimplification.

  1. Sex differences in risk factors for coronary heart disease: a study in a Brazilian population

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    Oliveira Helena CF

    2001-04-01

    Full Text Available Abstract Background In Brazil coronary heart disease (CHD constitutes the most important cause of death in both sexes in all the regions of the country and interestingly, the difference between the sexes in the CHD mortality rates is one of the smallest in the world because of high rates among women. Since a question has been raised about whether or how the incidence of several CHD risk factors differs between the sexes in Brazil the prevalence of various risk factors for CHD such as high blood cholesterol, diabetes mellitus, hypertension, obesity, sedentary lifestyle and cigarette smoking was compared between the sexes in a Brazilian population; also the relationships between blood cholesterol and the other risk factors were evaluated. Results The population presented high frequencies of all the risk factors evaluated. High blood cholesterol (CHOL and hypertension were more prevalent among women as compared to men. Hypertension, diabetes and smoking showed equal or higher prevalence in women in pre-menopausal ages as compared to men. Obesity and physical inactivity were equally prevalent in both sexes respectively in the postmenopausal age group and at all ages. CHOL was associated with BMI, sex, age, hypertension and physical inactivity. Conclusions In this population the high prevalence of the CHD risk factors indicated that there is an urgent need for its control; the higher or equal prevalences of several risk factors in women could in part explain the high rates of mortality from CHD in females as compared to males.

  2. Frequency of the CCR5-delta32 allele in Brazilian populations: A systematic literature review and meta-analysis.

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    Silva-Carvalho, Wlisses Henrique Veloso; de Moura, Ronald Rodrigues; Coelho, Antonio Victor Campos; Crovella, Sergio; Guimarães, Rafael Lima

    2016-09-01

    The CCR5 is a chemokine receptor widely expressed by several immune cells that are engaged in inflammatory responses. Some populations have individuals exhibiting a 32bp deletion in the CCR5 gene (CCR5-delta32) that produces a truncated non-functional protein not expressed on the cell surface. This polymorphism, known to be associated with susceptibility to infectious and inflammatory diseases, such as osteomyelitis, pre-eclampsia, systemic lupus erythematous, juvenile idiopathic arthritis, rheumatoid arthritis and HIV/AIDS, is more commonly found in European populations with average frequency of 10%. However, it is also possible to observe a significant frequency in other world populations, such as the Brazilian one. We performed a systematic review and meta-analysis of CCR5-delta32 genetic association studies in Brazilian populations throughout the country to estimate the frequency of this polymorphism. We also compared CCR5-delta32 frequencies across Brazilian regions. The systematic literature reviewed studies involving delta32 allele in Brazilian populations published from 1995 to 2015. Among the reviewed literature, 25 studies including 30 Brazilian populations distributed between the North, Northeast, South and Southeast regions were included in our meta-analysis. We observed an overall allelic frequency of 4% (95%-CI, 0.03-0.05), that was considered moderate and, notably, higher than some European populations, such as Cyprus (2.8%), Italy (3%) and Greece (2.4%). Regarding the regional frequency comparisons between North-Northeast (N-NE) and South-Southeast (S-SE) regions, we observed an allelic frequency of 3% (95%-CI, 0.02-0.04) and 4% (95%-CI, 0.03-0.05), respectively. The populations from S-SE regions had a slightly higher CCR5-delta32 frequency than N-NE regions (OR=1.41, p=0.002). Although there are several studies about the CCR5-delta32 polymorphism and its effect on the immune response of some infectious diseases, this report is the first meta

  3. Apolipoprotein E polymorphism distribution in an elderly Brazilian population: the Bambuí Health and Aging Study

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    A.K. Fuzikawa

    2007-11-01

    Full Text Available Apolipoprotein E (ApoE is one of the most extensively studied genes in the context of aging, but there are few population-based studies on ApoE polymorphism in the elderly in developing countries. The objective of the present study was to assess ApoE allele and genotype distribution in a large elderly community-based sample and its association with age, sex and skin color. Participants included 1408 subjects (80.8% of all residents aged ³60 years residing in Bambuí city, MG, Brazil. The DNA samples were subjected to the polymerase chain reaction amplification, followed by the restriction fragment length polymorphism technique, with digestion by HhaI. Analysis was carried out taking into consideration the six ApoE genotypes (e3/e3, e3/e4, e2/e3, e4/e4, e2/e4, and e2/e2, the three ApoE alleles, and the number of ApoE4 alleles for each individual. The e3 allele predominated (80.0%, followed by e4 (13.5% and e2 (6.5%. All six possible genotypes were observed, the e3/e3 genotype being the most frequent (63.4%. This distribution was similar to that described in other western populations. Sex was not associated with number of ApoE4 alleles. Black skin color was significantly and independently associated with the presence of two ApoE4 alleles (age-sex adjusted OR = 7.38; 95%CI = 1.93-28.25, showing that the African-Brazilian elderly have a high prevalence of the e4 allele, as observed in blacks from Africa. No association between number of ApoE4 alleles and age was found, suggesting the absence of association of ApoE genotype with mortality in this population.

  4. Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

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    Ádila Liliane Barros Dias

    2012-02-01

    Full Text Available INTRODUCTION: Hepatitis B virus (HBV infection is a serious public health issue worldwide. Hepatitis B virus is classified into eight genotypes, varying from A to H, with distinct geographical distributions. In Brazil, the most frequent genotypes are A, D, and F. METHODS: This study aimed to characterize the HBV genotypes in cases of hepatitis B virus and hepatitis D virus (HDV co-infections in an endemic area in the Western Brazilian Amazon. We analyzed 86 serum samples reactive for HBsAg from indigenous and non-indigenous populations obtained from previous serological surveys. RESULTS: Of the 86 reactive serum samples, 39 were found to be HBV-DNA-positive by semi-nested PCR. The genotypes were established by sequencing the amplified S gene region. We obtained 20 sequences classified into three genotypes: A, D, and F. Genotype A was the most frequent (60%, followed by D (35% and F (5%. CONCLUSIONS: The distribution of the HBV genotypes reflected the pattern of historical occupation of the region.

  5. TP53 codon 72 polymorphism as a risk factor for cardiovascular disease in a Brazilian population

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    M.A.C. Smith

    2007-11-01

    Full Text Available TP53, a tumor suppressor gene, has a critical role in cell cycle, apoptosis and cell senescence and participates in many crucial physiological and pathological processes. Identification of TP53 polymorphism in older people and age-related diseases may provide an understanding of its physiology and pathophysiological role as well as risk factors for complex diseases. TP53 codon 72 (TP53:72 polymorphism was investigated in 383 individuals aged 66 to 97 years in a cohort from a Brazilian Elderly Longitudinal Study. We investigated allele frequency, genotype distribution and allele association with morbidities such as cardiovascular disease, type II diabetes, obesity, neoplasia, low cognitive level (dementia, and depression. We also determined the association of this polymorphism with serum lipid fractions and urea, creatinine, albumin, fasting glucose, and glycated hemoglobin levels. DNA was isolated from blood cells, amplified by PCR using sense 5'-TTGCCGTCCCAAGCAATGGATGA-3' and antisense 5'-TCTGGGAAGGGACAGAAGATGAC-3' primers and digested with the BstUI enzyme. This polymorphism is within exon 4 at nucleotide residue 347. Descriptive statistics, logistic regression analysis and Student t-test using the multiple comparison test were used. Allele frequencies, R (Arg = 0.69 and P (Pro = 0.31, were similar to other populations. Genotype distributions were within Hardy-Weinberg equilibrium. This polymorphism did not show significant association with any age-related disease or serum variables. However, R allele carriers showed lower HDL levels and a higher frequency of cardiovascular disease than P allele subjects. These findings may help to elucidate the physiopathological role of TP53:72 polymorphism in Brazilian elderly people.

  6. Quality of life in a Brazilian sample of patients with Parkinson's disease and their caregivers

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    Schestatsky Pedro

    2006-01-01

    Full Text Available OBJECTIVE: Parkinson's disease is a common neurodegenerative disorder characterized by motor disabilities and increasing dependence on others for daily life activities with consequent impact on patients' and caregivers' quality of life. METHOD: A cross-sectional study was performed in which quality of life was assessed by the WHOQOL-BREF questionnaire in 21 patients with Parkinson's disease and their respective caregivers. RESULTS: Significant differences between patients and caregivers were found in physical (p < 0.001 and psychological (p = 0.002 domains. In the Parkinson's disease group there was a significant inverse correlation between the psychological domain and duration of disease (p = 0.01, as well as between social domain and severity of disease (p = 0.001. There was a positive correlation between physical domain scores and number of people living in the same house (p = 0.02. The only significant finding in the group of caregivers was an inverse correlation between the social domain and the patients age (p = 0.04. CONCLUSION: Duration, severity of the disease and the number of people living in the same house were the most important predictors of quality of life of Parkinson's disease patients. The age of the patients was the only significant predictor found in the caregivers' quality of life. In order to complement our findings, further short-form questionnaires should be validated for Brazilian samples of Parkinson's disease.

  7. Psychometric properties of the dimensional anxiety scales for DSM-5 in a Brazilian community sample.

    Science.gov (United States)

    DeSousa, Diogo A; Moreno, André L; Osório, Flávia L; Crippa, José Alexandre S; LeBeau, Richard; Manfro, Gisele G; Salum, Giovanni A; Koller, Silvia H

    2016-10-16

    The DSM-5 highlights the use of dimensional assessments of mental health as a supplement to categorical diagnoses. This study investigated the psychometric properties of the DSM-5 Dimensional Anxiety Scales in a Brazilian community sample. Dimensional scales for generalized anxiety disorder, social anxiety disorder, panic disorder, agoraphobia, and specific phobia were administered to 930 adults aged 18 to 70, 64.2% female. Psychometric properties investigated were: unidimensionality; measurement invariance; internal consistency; composite reliability; test-retest reliability; convergent and divergent validity; category thresholds and item performance analyses. Analyses revealed unidimensionality for all scales except for specific phobia. Measurement invariance, high internal consistency and composite reliability, and convergent and divergent validity were demonstrated. Test-retest reliability was high for all scales but generalized anxiety disorder. Item-based analyses evidenced that none of the items were very easy to endorse and that the scales offered more information about subjects with high severity estimates of anxiety. The DSM-5 Dimensional Anxiety Scales are a valid and reliable alternative to assess anxiety symptomatology in community settings, although further evaluation is needed, especially for specific phobia. The scales seem to be more useful for characterizing dimensionality of symptoms for subclinical or clinical cases than for slight or mildly anxious subjects.

  8. [Characteristics associated with the use of dental services by the adult Brazilian population].

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    Pinto, Rafaela da Silveira; Matos, Divane Leite; de Loyola Filho, Antônio Ignácio

    2012-02-01

    The scope of this study was to investigate the factors related to the use of dental services by Brazilian adults. Data were collected from 13,356 adults (35 to 44 years of age), participating in a nationwide epidemiological survey of oral health (SB-BRASIL 2003 Project). Data analysis was based on Poisson regression, which produced estimates of Prevalence Ratios as a measure of association. Data analysis showed that the use of dental services by adults was associated with: female gender, low education and income, living in the Northeastern and Southern regions and in small cities, complaints of toothache or gum pain, need for partial/total prosthesis, a greater amount of permanent teeth requiring treatment, demand for service due to some dental problems and evaluation of dental care received on a regular basis. These results showed that the population attended by the public service was socio-economically less privileged and had greater need for treatment. This situation reflects an historical abandonment of the adult population by the dental healthcare system in Brazil and poses a major challenge to the Unified Health Service, in light of its intended role to reduce inequalities and provide universal access to comprehensive care.

  9. Spatial and temporal country-wide survey of temephos resistance in Brazilian populations of Aedes aegypti

    Science.gov (United States)

    Chediak, Mateus; G Pimenta, Fabiano; Coelho, Giovanini E; Braga, Ima A; Lima, José Bento P; Cavalcante, Karina Ribeiro LJ; de Sousa, Lindemberg C; de Melo-Santos, Maria Alice V; Macoris, Maria de Lourdes da G; de Araújo, Ana Paula; Ayres, Constância Flávia J; Andrighetti, Maria Teresa M; Gomes, Ricristhi Gonçalves de A; Campos, Kauara B; Guedes, Raul Narciso C

    2016-01-01

    The organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (Aedes aegypti) in Brazil since the mid-1980s. Reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. As resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in Ae. aegypti in Brazil for the last 12 years using discriminating temephos concentrations and the bioassay protocols of the World Health Organization. The mortality results obtained were subjected to spatial analysis for distance interpolation using semi-variance models to generate maps that depict the spread of temephos resistance in Brazil since 1999. The problem has been expanding. Since 2002-2003, approximately half the country has exhibited mosquito populations resistant to temephos. The frequency of temephos resistance and, likely, control failures, which start when the insecticide mortality level drops below 80%, has increased even further since 2004. Few parts of Brazil are able to achieve the target 80% efficacy threshold by 2010/2011, resulting in a significant risk of control failure by temephos in most of the country. The widespread resistance to temephos in Brazilian Ae. aegypti populations greatly compromise effective mosquito control efforts using this insecticide and indicates the urgent need to identify alternative insecticides aided by the preventive elimination of potential mosquito breeding sites. PMID:27143489

  10. MSX1 gene and nonsyndromic oral clefts in a Southern Brazilian population

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    L.T. Souza

    2013-08-01

    Full Text Available Nonsyndromic oral clefts (NSOC are the most common craniofacial birth defects in humans. The etiology of NSOC is complex, involving both genetic and environmental factors. Several genes that play a role in cellular proliferation, differentiation, and apoptosis have been associated with clefting. For example, variations in the homeobox gene family member MSX1, including a CA repeat located within its single intron, may play a role in clefting. The aim of this study was to investigate the association between MSX1 CA repeat polymorphism and NSOC in a Southern Brazilian population using a case-parent triad design. We studied 182 nuclear families with NSOC recruited from the Hospital de Clínicas de Porto Alegre in Southern Brazil. The polymorphic region was amplified by the polymerase chain reaction and analyzed by using an automated sequencer. Among the 182 families studied, four different alleles were observed, at frequencies of 0.057 (175 bp, 0.169 (173 bp, 0.096 (171 bp and 0.67 (169 bp. A transmission disequilibrium test with a family-based association test (FBAT software program was used for analysis. FBAT analysis showed overtransmission of the 169 bp allele in NSOC (P=0.0005. These results suggest that the CA repeat polymorphism of the MSX1 gene may play a role in risk of NSOC in populations from Southern Brazil.

  11. Spatial and temporal country-wide survey of temephos resistance in Brazilian populations of Aedes aegypti.

    Science.gov (United States)

    Chediak, Mateus; G Pimenta, Fabiano; Coelho, Giovanini E; Braga, Ima A; Lima, José Bento P; Cavalcante, Karina Ribeiro Lj; Sousa, Lindemberg C de; Melo-Santos, Maria Alice V de; Macoris, Maria de Lourdes da G; Araújo, Ana Paula de; Ayres, Constância Flávia J; Andrighetti, Maria Teresa M; Gomes, Ricristhi Gonçalves de A; Campos, Kauara B; Guedes, Raul Narciso C

    2016-05-01

    The organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (Aedes aegypti) in Brazil since the mid-1980s. Reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. As resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in Ae. aegypti in Brazil for the last 12 years using discriminating temephos concentrations and the bioassay protocols of the World Health Organization. The mortality results obtained were subjected to spatial analysis for distance interpolation using semi-variance models to generate maps that depict the spread of temephos resistance in Brazil since 1999. The problem has been expanding. Since 2002-2003, approximately half the country has exhibited mosquito populations resistant to temephos. The frequency of temephos resistance and, likely, control failures, which start when the insecticide mortality level drops below 80%, has increased even further since 2004. Few parts of Brazil are able to achieve the target 80% efficacy threshold by 2010/2011, resulting in a significant risk of control failure by temephos in most of the country. The widespread resistance to temephos in Brazilian Ae. aegypti populations greatly compromise effective mosquito control efforts using this insecticide and indicates the urgent need to identify alternative insecticides aided by the preventive elimination of potential mosquito breeding sites.

  12. MSX1 gene and nonsyndromic oral clefts in a Southern Brazilian population

    Energy Technology Data Exchange (ETDEWEB)

    Souza, L.T. [Laboratório de Medicina Genômica, Centro de Pesquisa Experimental, Hospital de Clinicas de Porto Alegre, Porto Alegre, RS (Brazil); Programa de Pós-Graduaçãoo em Saúde da Criança e do Adolescente, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Kowalski, T.W. [Laboratório de Medicina Genômica, Centro de Pesquisa Experimental, Hospital de Clinicas de Porto Alegre, Porto Alegre, RS (Brazil); Collares, M.V.M. [Universidade Federal do Rio Grande do Sul, Departamento de Cirurgia, Porto Alegre, RS, Brasil, Departamento de Cirurgia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Félix, T.M. [Laboratório de Medicina Genômica, Centro de Pesquisa Experimental, Hospital de Clinicas de Porto Alegre, Porto Alegre, RS (Brazil); Programa de Pós-Graduaçãoo em Saúde da Criança e do Adolescente, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Hospital de Clínicas de Porto Alegre, Serviço de Genética Médica, Porto Alegre, RS, Brasil, Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS (Brazil)

    2013-08-10

    Nonsyndromic oral clefts (NSOC) are the most common craniofacial birth defects in humans. The etiology of NSOC is complex, involving both genetic and environmental factors. Several genes that play a role in cellular proliferation, differentiation, and apoptosis have been associated with clefting. For example, variations in the homeobox gene family member MSX1, including a CA repeat located within its single intron, may play a role in clefting. The aim of this study was to investigate the association between MSX1 CA repeat polymorphism and NSOC in a Southern Brazilian population using a case-parent triad design. We studied 182 nuclear families with NSOC recruited from the Hospital de Clínicas de Porto Alegre in Southern Brazil. The polymorphic region was amplified by the polymerase chain reaction and analyzed by using an automated sequencer. Among the 182 families studied, four different alleles were observed, at frequencies of 0.057 (175 bp), 0.169 (173 bp), 0.096 (171 bp) and 0.67 (169 bp). A transmission disequilibrium test with a family-based association test (FBAT) software program was used for analysis. FBAT analysis showed overtransmission of the 169 bp allele in NSOC (P=0.0005). These results suggest that the CA repeat polymorphism of the MSX1 gene may play a role in risk of NSOC in populations from Southern Brazil.

  13. Optimisation of sampling windows design for population pharmacokinetic experiments.

    Science.gov (United States)

    Ogungbenro, Kayode; Aarons, Leon

    2008-08-01

    This paper describes an approach for optimising sampling windows for population pharmacokinetic experiments. Sampling windows designs are more practical in late phase drug development where patients are enrolled in many centres and in out-patient clinic settings. Collection of samples under the uncontrolled environment at these centres at fixed times may be problematic and can result in uninformative data. Population pharmacokinetic sampling windows design provides an opportunity to control when samples are collected by allowing some flexibility and yet provide satisfactory parameter estimation. This approach uses information obtained from previous experiments about the model and parameter estimates to optimise sampling windows for population pharmacokinetic experiments within a space of admissible sampling windows sequences. The optimisation is based on a continuous design and in addition to sampling windows the structure of the population design in terms of the proportion of subjects in elementary designs, number of elementary designs in the population design and number of sampling windows per elementary design is also optimised. The results obtained showed that optimal sampling windows designs obtained using this approach are very efficient for estimating population PK parameters and provide greater flexibility in terms of when samples are collected. The results obtained also showed that the generalized equivalence theorem holds for this approach.

  14. Validation of a subjective well-being inventory with youth and adolescents Argentinean, Mexican, and Brazilian Samples

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    del Pino Peña, Roberto; Almeida Da Silva Junior, Sergio Henrique; Aguiar de Souza, Marcos; Paris,Laura; Omar,Alicia

    2009-01-01

    The aim of this study was to adapt the Well-Being Inventory of Nacpal & Shell for using with Latinamerican youth and adolescents. The conceptual, semantic and operational equivalence of inventory prototype version was analized. The final version was administered to a sample of 1,891 students (730 Mexicans, 664 Argentines and 497 Brazilians) who also filled a set of recognized instruments developed to explore resilience, coping, sense of humor, emotional intelligence and cultural values. The e...

  15. Raising awareness of chronic kidney disease in a Brazilian urban population

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    M. Mazza Nascimento

    2009-08-01

    Full Text Available The incidence and prevalence of chronic kidney disease have been increasing in recent years in developing countries. The aim of this study was to report the results of a general chronic kidney disease awareness program applied to an urban population in a large Brazilian city. From January 2002 to January 2005 a total of 8883 individuals in the city of Curitiba (PR, Brazil were screened for hypertension, body mass index, hematuria, and proteinuria. A family history and previous medical diagnosis of hypertension and diabetes mellitus (DM were also recorded. Of the 8883 individuals assessed, 56% were women, subject median age was 47 years (range: 17-93 years and more than 90% were Caucasian. Thirty percent had signs of hematuria, 6% had proteinuria, and 3% had hematuria and proteinuria. The median of mean arterial pressure values was 93 mmHg (range: 71-135 mmHg and 16% of the population screened had a history of hypertension. A significant positive family history of both hypertension or DM was present in 42% (P < 0.0001; chi-square = 83.18 and 7% (P < 0.0001; chi-square = 161.31 of the hypertensive group, respectively. Finally, the prevalence of hypertension and DM was significantly higher in older individuals with proteinuria. In the present study, a higher prevalence of hematuria and proteinuria was found in older individuals with hypertension and diabetes compared to the general population. These data confirm the need for public awareness of renal disease in high-risk individuals.

  16. Evaluation of properties of the Vestibular Disorders Activities of Daily Living Scale (Brazilian version in an elderly population

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    Natalia A. Ricci

    2014-05-01

    Full Text Available Background: The Vestibular Disorders Activities of Daily Living Scale (VADL is considered an important subjective assessment to evaluate patients suffering from dizziness and imbalance. Although frequently used, its metric characteristics still require further investigation. Objective: This paper aims to analyze the psychometric properties of the Brazilian version of the VADL in an elderly population. Method: The sample comprises patients (≥65 years old with chronic dizziness resulting from vestibular disorders. For discriminant analysis, patients were compared to healthy subjects. All subjects answered the VADL-Brazil by interview. To examine the VADL validity, patients filled out the Dizziness Handicap Inventory (DHI and the ABC scale and were tested on the Dynamic Gait Index (DGI. To evaluate the VADL responsiveness, 20 patients were submitted to rehabilitation. Results: Patients (n=140 had a VADL total score of 4.1±1.6 points. Healthy subjects scored significantly less than patients in all the subscales and in the VADL total score. The VADL-Brazil was weakly correlated with the DHI and moderately to the ABC scale and the DGI. Instead of the original 3 subscales, factor analysis resulted in 6 factors. The VADL was capable of detecting changes after rehabilitation, which means that the instrument has responsiveness. Conclusions: This study provided more data about the psychometric properties and usefulness of the VADL-Brazil. The use of such a reliable and valid instrument increases the knowledge about disability in patients with vestibular disorders.

  17. Is the Brazilian pharmaceutical policy ensuring population access to essential medicines?

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    Bertoldi Andréa

    2012-03-01

    Full Text Available Abstract Background To evaluate medicine prices, availability and affordability in Brazil, considering the differences across three types of medicines (originator brands, generics and similar medicines and different types of facilities (private pharmacies, public sector pharmacies and “popular pharmacies”. Methods Data on prices and availability of 50 medicines were collected in 56 pharmacies across six cities in Southern Brazil using the World Health Organization / Health Action International methodology. Median prices obtained were divided by international reference prices to derive the median price ratio (MPR. Results In the private sector, prices were 8.6 MPR for similar medicines, 11.3 MRP for generics and 18.7 MRP for originator brands, respectively. Mean availability was 65%, 74% and 48% for originator brands, generics and similar medicines, respectively. In the public sector, mean availability of similar medicines was 2–7 times higher than that of generics. Mean overall availability in the public sector ranged from 68.8% to 81.7%. In “popular pharmacies”, mean availability was greater than 90% in all cities. Conclusions Availability of medicines in the public sector does not meet the challenge of supplying essential medicines to the entire population, as stated in the Brazilian constitution. This has unavoidable repercussions for affordability, particularly amongst the lower socio-economic strata.

  18. Performance of four ischemic stroke prognostic scores in a Brazilian population

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    Gustavo W. Kuster

    2016-02-01

    Full Text Available ABSTRACT Objective Ischemic stroke (IS prognostic scales may help clinicians in their clinical decisions. This study aimed to assess the performance of four IS prognostic scales in a Brazilian population. Method We evaluated data of IS patients admitted at Hospital Paulistano, a Joint Commission International certified primary stroke center. In-hospital mortality and modified Rankin score at discharge were defined as the outcome measures. The performance of National Institutes of Health Stroke Scale (NIHSS, Stroke Prognostication Using Age and NIHSS (SPAN-100, Acute Stroke Registry and Analysis of Lausanne (ASTRAL, and Totaled Health Risks in Vascular Events (THRIVE were compared. Results Two hundred six patients with a mean ± SD age of 67.58 ± 15.5 years, being 55.3% male, were included. The four scales were significantly and independently associated functional outcome. Only THRIVE was associated with in-hospital mortality. With area under the curve THRIVE and NIHSS were the scales with better performance for functional outcome and THRIVE had the best performance for mortality. Conclusion THRIVE showed the best performance among the four scales, being the only associated with in-hospital mortality.

  19. Prevalence of maternal group B streptococcal colonization and related risk factors in a Brazilian population

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    Alexander S. Zusman

    2006-08-01

    Full Text Available The objective of this study was to determine the prevalence of maternal group B Streptococcal (GBS colonization and compare risk factor data related to GBS colonization. A prospective surveillance study of 598 pregnant women was conducted in two socioeconomically diverse maternity hospitals in Ribeirão Preto, Brazil between June and October 1999. Swabs from the lower vagina were obtained between 35 and 37 weeks gestation and cultured on selective media. Risk factor data were obtained by patient interview and chart review. The overall maternal GBS colonization prevalence rate was 17.9%. There was no association of GBS colonization with maternity hospital and no association of GBS colonization with previously identified risk factors, such as age, race, martial status, maternal education, parity, smoking, or alcohol use. There is a relatively high prevalence of maternal GBS colonization in this Brazilian population, although previously-identified-risk factors were not found to be important. This study provides baseline data for the creation of community-based GBS disease prevention protocols.

  20. The relative frequency of odontogenic tumors: A study of 376 cases in a Brazilian population

    Science.gov (United States)

    Lima-Verde-Osterne, Rafael; Turatti, Eveline; Cordeiro-Teixeira, Renata

    2017-01-01

    Background Odontogenic tumors (OTs) are rare lesions, exclusive of the jaws, that are derived from epithelial and/or ectomesenchymal elements of the tooth-forming apparatus. Their biological behavior is heterogeneous, including hamartomatous tissue proliferation, benign nonaggressive and aggressive neoplasms, and malignant tumors with metastatic capacity. The aim of this study was to describe the relative frequency of odontogenic tumors in a Brazilian population. In addition, a review of the literature identified studies on odontogenic tumors that follow the 2005 World Health Organization. Material and Methods A total of 376 cases of odontogenic tumors from an oral pathology service were reviewed about age, gender, anatomic site and histologic diagnosis. Results Keratocystic odontogenic tumors (31.6%) were the most common, followed by ameloblastoma (28.5%), and odontoma (22.6%). The mean age was 32.2 years, and more than half the patients (52.1%) were in the second and third decades of life. The male to female ratio was 1:1.37, with a maxilla to mandible ratio of 1:2.08. Conclusions The variation in relative frequency of tumors observed among the several series, including the present study, is probably due in part to cultural differences between geographic areas but also to the study design. Key words:Pathology, epidemiology, odontogenic tumors. PMID:28160576

  1. Effectiveness of prediction equations in estimating energy expenditure sample of Brazilian and Spanish women with excess body weight

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    Eliane Lopes Rosado

    2014-03-01

    Full Text Available Objective: To assess the adequacy of predictive equations for estimation of energy expenditure (EE, compared with the EE using indirect calorimetry in a sample of Brazilian and Spanish women with excess body weight Methods: It is a cross-sectional study with 92 obese adult women [26 Brazilian -G1- and 66 Spanish - G2- (aged 20-50]. Weight and height were evaluated during fasting for the calculation of body mass index and predictive equations. EE was evaluated using the open-circuit indirect calorimetry with respiratory hood. Results: In G1 and G2, it was found that the estimates obtained by Harris-Benedict, Shofield, FAO/WHO/ ONU and Henry & Rees did not differ from EE using indirect calorimetry, which presented higher values than the equations proposed by Owen, Mifflin-St Jeor and Oxford. For G1 and G2 the predictive equation closest to the value obtained by the indirect calorimetry was the FAO/WHO/ONU (7.9% and 0.46% underestimation, respectively, followed by Harris-Benedict (8.6% and 1.5% underestimation, respectively. Conclusion: The equations proposed by FAO/WHO/ ONU, Harris-Benedict, Shofield and Henry & Rees were adequate to estimate the EE in a sample of Brazilian and Spanish women with excess body weight. The other equations underestimated the EE.

  2. Multiple sclerosis susceptibility in a Brazilian sample, HLA and CIITA genes

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    Eduardo Ribeiro Paradela

    2014-06-01

    Full Text Available Introduction: The multiple sclerosis (MS is a chronic disease of the central nervous system that affects mainly young adults. This disease is characterized by the spread of demyelinating lesions in time and space. This condition may be influenced by genetic factors as heterogeneity, incomplete penetrance, polygenic inheritance and epigenetic factors, which makes this complex disease a challenge for geneticists. Objectives: The aim of this study was to investigate the association between HLA alleles from DQA1, DQB1 and DRB1 loci (6p21.3, genetic polymorphisms -168A/G (rs3087456 and +1614 G/C (rs4774 in the CIITA gene (16p13 and susceptibility to MS in a miscegenated sample from Rio de Janeiro state, RJ, Brazil. Method: DNA samples from 52 patients with relapsing remitting multiple sclerosis (MSRR [21 males (40.38% and 31 females (59.62%] and 116 healthy controls [46 males (39.65% and 70 females (60.35%] matched by race, sex and age were analyzed by techniques of PCR, SSP-PCR, electrophoresis and DNA sequencing. Results: A significant association between MS and HLA-DRB1*15:01 allele was observed [p value=.002; Odds Ratio (OR=3.2], especially in women (p=.001; OR=4.9, which remained statistically significant after Bonferroni correction. Furthermore, it was observed that the polymorphism +1614 G/C, “C/C” profile, in association with the allele DRB1*15:01 influences the increased susceptibility to MS in women (p=.029; OR=5.6. In addition, it was observed that the "G/G" profile in CIITA polymorphism +1614G/C may be associated with the resistance to MS (p=.02; OR=0.23, as well as HLA-DRB1*11:02 (p=.02, OR=0.5. The HLA-DQB1*06:02 allele also has been implicated as a possible susceptibility factor for MS (p=.02; OR=1.8, data not confirmed after Bonferronís correction. Conclusion: Together, these results reinforce the polygenic nature of MS, and proposed that the CIITA gene, which is the regulator of the expression of HLA-D, is an additive factor to

  3. Disproportionate sampling for population subgroups in telephone surveys.

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    Kalsbeek, William D; Boyle, Walter R; Agans, Robert P; White, John E

    2007-04-15

    Population studies often seek to examine phenomena in important population subgroups or to compare results among these and other subgroups. When subgroups of interest comprise a relatively small percentage of the population and acceptable subgroup member lists are not available to serve as sampling frames, it may be prohibitively expensive even by telephone to screen through a sample of the entire population. This paper considers some statistical effects of estimation from a class of two-stratum telephone sample designs where part of the frame with a higher subgroup concentration is disproportionately sampled compared to the rest of the frame. Using proportionate sampling as a reference, the relative impact of this disproportionate design is determined for nominal and effective sample sizes, where the latter are tied to the effect of variation in sample weights that occurs in disproportionately allocated samples. Findings are illustrated using two recent telephone surveys. Whereas nominal subgroup sample sizes may be improved by disproportionate sampling, we conclude that both the survey designer and analyst should use this type of design cautiously in telephone surveys.

  4. Population Education in Home Economics: Some Sample Lessons.

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    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    Seven sample lessons on population and the family appear in this home economics teacher's manual. These activities have been adapted from materials produced in several countries in Asia and Oceania. A scope and sequence chart illustrates how teachers can integrate population-related issues into the home economics curriculum. Among the topics…

  5. Population Education in Social Studies: Some Sample Lessons.

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    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    This booklet contains sample lessons and learning materials from the countries of Asia and Oceania for teaching population education in social studies. The booklet is one of a series of six, each of which brings out population education concepts as part of a particular subject area. The subject areas treated in the other booklets are home…

  6. Estimating hidden population size using Respondent-Driven Sampling data.

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    Handcock, Mark S; Gile, Krista J; Mar, Corinne M

    Respondent-Driven Sampling (RDS) is n approach to sampling design and inference in hard-to-reach human populations. It is often used in situations where the target population is rare and/or stigmatized in the larger population, so that it is prohibitively expensive to contact them through the available frames. Common examples include injecting drug users, men who have sex with men, and female sex workers. Most analysis of RDS data has focused on estimating aggregate characteristics, such as disease prevalence. However, RDS is often conducted in settings where the population size is unknown and of great independent interest. This paper presents an approach to estimating the size of a target population based on data collected through RDS. The proposed approach uses a successive sampling approximation to RDS to leverage information in the ordered sequence of observed personal network sizes. The inference uses the Bayesian framework, allowing for the incorporation of prior knowledge. A flexible class of priors for the population size is used that aids elicitation. An extensive simulation study provides insight into the performance of the method for estimating population size under a broad range of conditions. A further study shows the approach also improves estimation of aggregate characteristics. Finally, the method demonstrates sensible results when used to estimate the size of known networked populations from the National Longitudinal Study of Adolescent Health, and when used to estimate the size of a hard-to-reach population at high risk for HIV.

  7. Populations analysis of the Brazilian Sharpnose Shark Rhizoprionodon lalandii (Chondrichthyes: Carcharhinidae on the São Paulo coast, Southern Brazil: inferences from mt DNA sequences

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    Fernando Fernandes Mendonça

    Full Text Available Sharks of the genus Rhizoprionodon can be considered some of the most important predators along the trophic coastal marine ecosystems and represent an important economic resource for the small-scale fisheries, especially on the Brazilian coastline. In order to analyze the population structure of the shark Rhizoprionodon lalandii of São Paulo, Southeastern coast of Brazil, levels of genetic diversity were identified by nucleotide sequence analyses of the mitochondrial DNA control region. The results obtained from this study present moderate values of haplotype diversity and low nucleotide diversity. Although the AMOVA tests (ΦST = 0.08394, P < 0.01 had shown slightly differences among the studied samples, evidence for the occurrence of population structuring was not found, which may be a general feature of sharks living in coastal areas.

  8. Mortality due to diseases of the circulatory system among the elderly population in Brazilian Amazon: temporal and spatial analysis

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    Karine Vila Real Nunes

    2013-12-01

    Full Text Available INTRODUCTION: Circulatory Diseases (CD are the major cause of death among the elderly population in Brazilian Amazon. OBJECTIVE: to analyze standardized mortality rates of diseases of the circulatory system (DCS, according to the main causes of death among the elderly, in microregions of the Brazilian Amazon, in the period of 1998 - 2007. METHODS: ecological study of mortality rates distribution standardized by CD and corrected by deaths from poorly defined causes among the elderly (> 65 years of age who lived in Brazilian Amazon in the period of 1998 - 2007. The analysis were carried out by the linear regression, trend, and spatial distribution of Kernel. RESULTS: We verified an increasing trend in mortality by CD (β1 = 28.34 p = 0.01, due to the increasing trend in the States of Maranhão and Tocantins. The central region of Mato Grosso, Northern Tocantins, Eastern Pará and Southwestern Maranhão present hot spots with the highest mortality rates. Males present higher rates when compared to females all over the region; rates of mortality due to acute myocardial infarction and hypertensive disease present the same spatial standard of the CD group and the rates of cerebrovascular diseases present a different spatial distribution standard. Increment in mortality rates according to age was observed: the greater the age, the higher is mortality by CD. CONCLUSION: The Brazilian Amazon presents an increasing trend with high rates of mortality by the circulatory diseases, and the geographic areas with the highest rates are around the Brazilian Amazon, in the states of Tocantins, Maranhão and Mato Grosso.

  9. Historical sampling reveals dramatic demographic changes in western gorilla populations

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    Guschanski Katerina

    2011-04-01

    Full Text Available Abstract Background Today many large mammals live in small, fragmented populations, but it is often unclear whether this subdivision is the result of long-term or recent events. Demographic modeling using genetic data can estimate changes in long-term population sizes while temporal sampling provides a way to compare genetic variation present today with that sampled in the past. In order to better understand the dynamics associated with the divergences of great ape populations, these analytical approaches were applied to western gorillas (Gorilla gorilla and in particular to the isolated and Critically Endangered Cross River gorilla subspecies (G. g. diehli. Results We used microsatellite genotypes from museum specimens and contemporary samples of Cross River gorillas to infer both the long-term and recent population history. We find that Cross River gorillas diverged from the ancestral western gorilla population ~17,800 years ago (95% HDI: 760, 63,245 years. However, gene flow ceased only ~420 years ago (95% HDI: 200, 16,256 years, followed by a bottleneck beginning ~320 years ago (95% HDI: 200, 2,825 years that caused a 60-fold decrease in the effective population size of Cross River gorillas. Direct comparison of heterozygosity estimates from museum and contemporary samples suggests a loss of genetic variation over the last 100 years. Conclusions The composite history of western gorillas could plausibly be explained by climatic oscillations inducing environmental changes in western equatorial Africa that would have allowed gorilla populations to expand over time but ultimately isolate the Cross River gorillas, which thereafter exhibited a dramatic population size reduction. The recent decrease in the Cross River population is accordingly most likely attributable to increasing anthropogenic pressure over the last several hundred years. Isolation of diverging populations with prolonged concomitant gene flow, but not secondary admixture, appears

  10. Structural validity and reliability of the Positive and Negative Affect Schedule (PANAS: Evidence from a large Brazilian community sample

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    Hudson W. de Carvalho

    2013-06-01

    Full Text Available Objective: Positive and negative affect are the two psychobiological-dispositional dimensions reflecting proneness to positive and negative activation that influence the extent to which individuals experience life events as joyful or as distressful. The Positive and Negative Affect Schedule (PANAS is a structured questionnaire that provides independent indexes of positive and negative affect. This study aimed to validate a Brazilian interview-version of the PANAS by means of factor and internal consistency analysis. Methods: A representative community sample of 3,728 individuals residing in the cities of São Paulo and Rio de Janeiro, Brazil, voluntarily completed the PANAS. Exploratory structural equation model analysis was based on maximum likelihood estimation and reliability was calculated via Cronbach's alpha coefficient. Results: Our results provide support for the hypothesis that the PANAS reliably measures two distinct dimensions of positive and negative affect. Conclusion: The structure and reliability of the Brazilian version of the PANAS are consistent with those of its original version. Taken together, these results attest the validity of the Brazilian adaptation of the instrument.

  11. alpha-Globin genes: thalassemic and structural alterations in a Brazilian population

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    M.R.S.C. Wenning

    2000-09-01

    Full Text Available Seven unrelated patients with hemoglobin (Hb H disease and 27 individuals with alpha-chain structural alterations were studied to identify the alpha-globin gene mutations present in the population of Southeast Brazil. The -alpha3.7, --MED and -(alpha20.5 deletions were investigated by PCR, whereas non-deletional alpha-thalassemia (alphaHphalpha, alphaNcoIalpha, aaNcoI, alphaIcalpha and alphaTSaudialpha was screened with restriction enzymes and by nested PCR. Structural alterations were identified by direct DNA sequencing. Of the seven patients with Hb H disease, all of Italian descent, two had the -(alpha20.5/-alpha3.7 genotype, one had the --MED/-alpha3.7 genotype, one had the --MED/alphaHphalpha genotype and three showed interaction of the -alpha3.7 deletion with an unusual, unidentified form of non-deletional alpha-thalassemia [-alpha3.7/(aaT]. Among the 27 patients with structural alterations, 15 (of Italian descent had Hb Hasharon (alpha47Asp->His associated with the -alpha3.7 deletion, 4 (of Italian descent were heterozygous for Hb J-Rovigo (alpha53Ala->Asp, 4 (3 Blacks and 1 Caucasian were heterozygous for Hb Stanleyville-II (alpha78Asn->Lys associated with the alpha+-thalassemia, 1 (Black was heterozygous for Hb G-Pest (alpha74Asp->Asn, 1 (Caucasian was heterozygous for Hb Kurosaki (alpha7Lys->Glu, 1 (Caucasian was heterozygous for Hb Westmead (alpha122His->Gln, and 1 (Caucasian was the carrier of a novel silent variant (Hb Campinas, alpha26Ala->Val. Most of the mutations found reflected the Mediterranean and African origins of the population. Hbs G-Pest and Kurosaki, very rare, and Hb Westmead, common in southern China, were initially described in individuals of ethnic origin differing from those of the carriers reported in the present study and are the first cases to be reported in the Brazilian population.

  12. COMPARISON OF SAMPLING TECHNIQUES USED IN STUDYING LEPIDOPTERA POPULATION DYNAMICS

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    Four methods (light traps, foliage samples, canvas bands, and gypsy moth egg mass surveys) that are used to study the population dynamics of foliage-feeding Lepidoptera were compared for 10 species, including gypsy moth, Lymantria dispar L. Samples were collected weekly at 12 sit...

  13. Probability Sampling Method for a Hidden Population Using Respondent-Driven Sampling: Simulation for Cancer Survivors.

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    Jung, Minsoo

    2015-01-01

    When there is no sampling frame within a certain group or the group is concerned that making its population public would bring social stigma, we say the population is hidden. It is difficult to approach this kind of population survey-methodologically because the response rate is low and its members are not quite honest with their responses when probability sampling is used. The only alternative known to address the problems caused by previous methods such as snowball sampling is respondent-driven sampling (RDS), which was developed by Heckathorn and his colleagues. RDS is based on a Markov chain, and uses the social network information of the respondent. This characteristic allows for probability sampling when we survey a hidden population. We verified through computer simulation whether RDS can be used on a hidden population of cancer survivors. According to the simulation results of this thesis, the chain-referral sampling of RDS tends to minimize as the sample gets bigger, and it becomes stabilized as the wave progresses. Therefore, it shows that the final sample information can be completely independent from the initial seeds if a certain level of sample size is secured even if the initial seeds were selected through convenient sampling. Thus, RDS can be considered as an alternative which can improve upon both key informant sampling and ethnographic surveys, and it needs to be utilized for various cases domestically as well.

  14. Fire effects on the population structure of Zanthoxylum rhoifolium Lam (Rutaceae) in a Brazilian savanna.

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    Silva, I A; Valenti, M W; Silva-Matos, D M

    2009-08-01

    Since cerrado fires may impede the growth of seedlings into trees, they may shape the population of woody species. In this study, we assessed the effects of a severe fire on the population structure and spatial distribution of Zanthoxylum rhoifolium, a widespread cerrado tree. We were interested to know the importance of the resprouting and sexual reproduction in regenerating the population. The study area had been for about six years protected from fire, before a severe fire at the end of the dry season in 2006. We sampled and measured all individuals of Z. rhoifolium found in 80 plots of 25 m(2). We found 149 individuals before the fire and 112 after the fire, of which 77 were resprouts from burned seedlings and saplings. We did not find significant differences between the population structure before and after the fire. The spatial distribution of the population remained clumped after the fire. Thus, the Z. rhoifolium population was very resilient to a severe fire. We did not find any new seedlings. As a consequence, resprouting seems to be more important than sexual reproduction in promptly regenerating the Z. rhoifolium populations.

  15. Fire effects on the population structure of Zanthoxylum rhoifolium Lam (Rutaceae in a Brazilian savanna

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    IA. Silva

    Full Text Available Since cerrado fires may impede the growth of seedlings into trees, they may shape the population of woody species. In this study, we assessed the effects of a severe fire on the population structure and spatial distribution of Zanthoxylum rhoifolium, a widespread cerrado tree. We were interested to know the importance of the resprouting and sexual reproduction in regenerating the population. The study area had been for about six years protected from fire, before a severe fire at the end of the dry season in 2006. We sampled and measured all individuals of Z. rhoifolium found in 80 plots of 25 m². We found 149 individuals before the fire and 112 after the fire, of which 77 were resprouts from burned seedlings and saplings. We did not find significant differences between the population structure before and after the fire. The spatial distribution of the population remained clumped after the fire. Thus, the Z. rhoifolium population was very resilient to a severe fire. We did not find any new seedlings. As a consequence, resprouting seems to be more important than sexual reproduction in promptly regenerating the Z. rhoifolium populations.

  16. Comparison of postmenopausal endogenous sex hormones among Japanese, Japanese Brazilians, and non-Japanese Brazilians

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    Maciel Maria

    2011-02-01

    Full Text Available Abstract Background Differences in sex hormone levels among populations might contribute to the variation in breast cancer incidence across countries. Previous studies have shown higher breast cancer incidence and mortality among Japanese Brazilians than among Japanese. To clarify the difference in hormone levels among populations, we compared postmenopausal endogenous sex hormone levels among Japanese living in Japan, Japanese Brazilians living in the state of São Paulo, and non-Japanese Brazilians living in the state of São Paulo. Methods A cross-sectional study was conducted using a control group of case-control studies in Nagano, Japan, and São Paulo, Brazil. Participants were postmenopausal women older than 55 years of age who provided blood samples. We measured estradiol, estrone, androstenedione, dehydroepiandrosterone sulfate (DHEAS, testosterone and free testosterone by radioimmunoassay; bioavailable estradiol by the ammonium sulfate precipitation method; and sex hormone-binding globulin (SHBG by immunoradiometric assay. A total of 363 women were included for the present analyses, comprising 185 Japanese, 44 Japanese Brazilians and 134 non-Japanese Brazilians. Results Japanese Brazilians had significantly higher levels of estradiol, bioavailable estradiol, estrone, testosterone and free testosterone levels, and lower SHBG levels, than Japanese. Japanese Brazilians also had significantly higher levels of bioavailable estradiol, estrone and DHEAS and lower levels of SHBG and androstenedione than non-Japanese Brazilians. Levels of estradiol, testosterone and free testosterone, however, did not differ between Japanese Brazilians and non-Japanese Brazilians. These differences were observed even after adjustment for known breast cancer risk factors. We also found an increase in estrogen and androgen levels with increasing body mass index, but no association for most of the other known risk factors. Conclusions We found higher levels of

  17. Disentangling seasonal bacterioplankton population dynamics by high-frequency sampling.

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    Lindh, Markus V; Sjöstedt, Johanna; Andersson, Anders F; Baltar, Federico; Hugerth, Luisa W; Lundin, Daniel; Muthusamy, Saraladevi; Legrand, Catherine; Pinhassi, Jarone

    2015-07-01

    Multiyear comparisons of bacterioplankton succession reveal that environmental conditions drive community shifts with repeatable patterns between years. However, corresponding insight into bacterioplankton dynamics at a temporal resolution relevant for detailed examination of variation and characteristics of specific populations within years is essentially lacking. During 1 year, we collected 46 samples in the Baltic Sea for assessing bacterial community composition by 16S rRNA gene pyrosequencing (nearly twice weekly during productive season). Beta-diversity analysis showed distinct clustering of samples, attributable to seemingly synchronous temporal transitions among populations (populations defined by 97% 16S rRNA gene sequence identity). A wide spectrum of bacterioplankton dynamics was evident, where divergent temporal patterns resulted both from pronounced differences in relative abundance and presence/absence of populations. Rates of change in relative abundance calculated for individual populations ranged from 0.23 to 1.79 day(-1) . Populations that were persistently dominant, transiently abundant or generally rare were found in several major bacterial groups, implying evolution has favoured a similar variety of life strategies within these groups. These findings suggest that high temporal resolution sampling allows constraining the timescales and frequencies at which distinct populations transition between being abundant or rare, thus potentially providing clues about physical, chemical or biological forcing on bacterioplankton community structure.

  18. Validation of a physical anthropology methodology using mandibles for gender estimation in a Brazilian population

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    Suzana Papile Maciel Carvalho

    2013-07-01

    Full Text Available Validation studies of physical anthropology methods in the different population groups are extremely important, especially in cases in which the population variations may cause problems in the identification of a native individual by the application of norms developed for different communities. OBJECTIVE: This study aimed to estimate the gender of skeletons by application of the method of Oliveira, et al. (1995, previously used in a population sample from Northeast Brazil. MATERIAL AND METHODS: The accuracy of this method was assessed for a population from Southeast Brazil and validated by statistical tests. The method used two mandibular measurements, namely the bigonial distance and the mandibular ramus height. The sample was composed of 66 skulls and the method was applied by two examiners. The results were statistically analyzed by the paired t test, logistic discriminant analysis and logistic regression. RESULTS: The results demonstrated that the application of the method of Oliveira, et al. (1995 in this population achieved very different outcomes between genders, with 100% for females and only 11% for males, which may be explained by ethnic differences. However, statistical adjustment of measurement data for the population analyzed allowed accuracy of 76.47% for males and 78.13% for females, with the creation of a new discriminant formula. CONCLUSION: It was concluded that methods involving physical anthropology present high rate of accuracy for human identification, easy application, low cost and simplicity; however, the methodologies must be validated for the different populations due to differences in ethnic patterns, which are directly related to the phenotypic aspects. In this specific case, the method of Oliveira, et al. (1995 presented good accuracy and may be used for gender estimation in Brazil in two geographic regions, namely Northeast and Southeast; however, for other regions of the country (North, Central West and South

  19. Validation of the Social Phobia and Anxiety Inventory for Children (SPAI-C in a sample of Brazilian children

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    G.J.C. Gauer

    2005-05-01

    Full Text Available The purpose of the present study was to examine the factor structure and psychometric properties of the Social Phobia and Anxiety Inventory for Children (SPAI-C, an instrument developed in the United States and applied to a sample of Brazilian schoolchildren. The process included the translation of the original material from English into Portuguese by two bilingual psychiatrists and a back translation by a bilingual physician. Both the front and back translations were revised by a bilingual child psychiatrist. The study was performed using a cross-sectional design and the Portuguese version of the SPAI-C was applied to a sample of 1954 children enrolled in 3rd to 8th grade attending 2 private and 11 public schools. Eighty-one subjects were excluded due to an incomplete questionnaire and 2 children refused to participate. The final sample consisted of 1871 children, 938 girls (50.1% and 933 boys (49.8%, ranging in age from 9 to 14 years. The majority of the students were Caucasian (89.0% and the remainder were African-Brazilian (11.0%. The Pearson product-moment correlation showed that the two-week test-retest reliability coefficient was r = 0.780 and Cronbach's alpha was 0.946. The factor structure was almost similar to that reported in previous studies. The results regarding the internal consistency, the test-retest reliability and the factor structure were similar to the findings obtained in studies performed on English speaking children. The present study showed that the Portuguese language version of SPAI-C is a reliable and valid measure of social anxiety for Brazilian children.

  20. Field studies of a Brazilian seahorse population, Hippocampus reidi Ginsburg, 1933

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    Natalie Villar Freret-Meurer

    2008-08-01

    Full Text Available This study was carried out to fill the gaps that remain under Hippocampus reidi biology. Analysis of variations of sex ratio, density, breeding season, distribution and home range of a population of the endangered Brazilian seahorse H. reidi from a rocky shore on Araçatiba beach, Ilha Grande, Brazil were carried out. Araçatiba beach is a tourist Environmental Protected Area, suffering antropic pressure. A fixed population of H. reidi was studied, where al lthe individuals were visually tagged and sex, reproductive state and location on site were identified from December 2002 to November 2004. A total of 20 individuals were visually tagged with a mean density of 0.18 m-2. Sex ratios were skewed, with more females than males. All the males brooded during 13 months and presented smaller home range than the females during the breeding season. The highest densities were found on shallowest areas.Este estudo foi realizado com o objetivo de preencher algumas lacunas sobre a biologia natural de Hippocampus reidi. Foram analisadas as variações na proporção sexual e densidade, período reprodutivo, distribuição e área de deslocamento de uma população do cavalo marinho brasileiro ameaçado de extinção Hippocampus reidi de um costão rochoso da praia de Araçatiba, Ilha Grande, Brasil. A praia de Araçatiba é uma Área de Proteção Ambiental turística, a qual sofre com a pressão antropogênica. Foi estudada uma população fixa de H. reidi, sendo que todos os indivíduos foram marcados visualmente e foram determinados o sexo, o período reprodutivo e a localização na área de novembro de 2002 a novembro de 2004. Um total de 20 indivíduos foram marcados com uma densidade média na área de 0,18 m-2. A proporção sexual variou de forma alternada com maior número de fêmeas que de machos. Durante 13 meses todos os machos encontrados estavam incubando. Os cavalos marinhos apresentaram área de deslocamento menor que as fêmeas durante o

  1. Prevalence of neurocognitive disorders and depression in a Brazilian HIV population

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    Flávio Trentin Troncoso

    2015-08-01

    Full Text Available AbstractINTRODUCTION:Combined antiretroviral therapy has enabled human immunodeficiency virus (HIV carriers to live longer. This increased life expectancy is associated with the occurrence of degenerative diseases, including HIV-associated neurocognitive disorders (HAND, which are diagnosed via a complex neuropsychological assessment. The International HIV Dementia Scale (IHDS is a screening instrument validated in Brazil for use in the absence of neuropsychological evaluation. HIV patients are frequently diagnosed with depression. We aimed to determine the prevalence of neurocognitive impairment using the IHDS and depressive disorders using the Hamilton Rating Scale for Depression (HAM-D17, compare the IHDS performance with the performances on the Timed Gait Test (TGT, the Digit Symbol Coding Test (DS and the Brazilian version of the Scale of Instrumental Activities of Daily Living (IADL, and evaluate the association between the IHDS performance and clinical-demographic variables.METHODS:One hundred fourteen patients were evaluated in a cross-sectional study conducted in a public outpatient clinic for infectious diseases in Marília City, State of São Paulo, Brazil. Data were collected following consultation. Statistical analysis was performed in accordance with the nature and distribution of the data and hypotheses.RESULTS:According to the IHDS, 53.2% of the sampled patients were neuropsychologically impaired. According to the HAM-D17, 26.3% had depressive disorders. There were significant associations between the IHDS and the TGT and DS. Multiple regression analysis indicated that female gender, educational level, and cluster of differentiation 4 (CD4 levels were significantly and independently associated with neurocognitive impairment.CONCLUSIONS:The prevalence of neurocognitive impairment according to the IHDS is high and associated with female gender, education level, and low CD4 levels.

  2. Clinical and radiological analysis of a series of periapical cysts and periapical granulomas diagnosed in a Brazilian population

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    Tavares, Daniel-Petitet; Rodrigues, Janderson-Teixeira; dos Santos, Teresa-Cristina-Ribeiro-Bartholomeu; Armada, Luciana

    2017-01-01

    Background Periapical cysts (PC) and periapical granulomas (PG) are the two most common chronic inflammatory periapical diseases, but their clinicoradiological characteristics can vary depending on the methods employed in each study. The aim of the present work was to analyze the clinical and radiological profile of a series of PC and PG diagnosed in a Brazilian population. Material and Methods The files of two Oral Pathology laboratories were reviewed and all cases diagnosed as PG and PC were selected for the study. Clinical and radiological information were retrieved and data were tabulated and descriptively and comparatively analyzed. Results Final sample was composed by 647 inflammatory periapical lesions, including 244 PG (38%) and 403 PC (62%). The number of women affected by PG was significantly higher than the number of women affected by PC (p=0.037). Anterior region of the maxilla was the most common affected area for both entities (39% of the cases), but the most common anatomical location of PG (anterior maxilla and posterior maxilla) was different from PC (anterior maxilla and posterior mandible) (p<0.0001). Upper lateral incisor was the most affected tooth. The mean radiological size of the PC was larger than the mean radiological size of the PG (p<0.0001) and PC showed well-defined radiological images more frequently than PG (p<0.0001). Conclusions PC were more common than PG, both showed predilection for adult females, most lesions affected predominantly the anterior maxilla and PC presented larger mean radiological diameter and well-defined images when compared with PG. Key words:Periapical granuloma, periapical cyst, radicular cyst, diagnosis, Oral Pathology. PMID:28149477

  3. Genotyping-by-sequencing approach indicates geographic distance as the main factor affecting genetic structure and gene flow in Brazilian populations of Grapholita molesta (Lepidoptera, Tortricidae).

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    Silva-Brandão, Karina Lucas; Silva, Oscar Arnaldo Batista Neto E; Brandão, Marcelo Mendes; Omoto, Celso; Sperling, Felix A H

    2015-06-01

    The oriental fruit moth Grapholita molesta is one of the major pests of stone and pome fruit species in Brazil. Here, we applied 1226 SNPs obtained by genotyping-by-sequencing to test whether host species associations or other factors such as geographic distance structured populations of this pest. Populations from the main areas of occurrence of G. molesta were sampled principally from peach and apple orchards. Three main clusters were recovered by neighbor-joining analysis, all defined by geographic proximity between sampling localities. Overall genetic structure inferred by a nonhierarchical amova resulted in a significant ΦST value = 0.19109. Here, we demonstrate for the first time that SNPs gathered by genotyping-by-sequencing can be used to infer genetic structure of a pest insect in Brazil; moreover, our results indicate that those markers are very informative even over a restricted geographic scale. We also demonstrate that host plant association has little effect on genetic structure among Brazilian populations of G. molesta; on the other hand, reduced gene flow promoted by geographic isolation has a stronger impact on population differentiation.

  4. Genetic differentiation in geographically close populations of the water rat Nectomys squamipes (Rodentia, Sigmodontinae from the Brazilian Atlantic Forest

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    Maroja L.S.

    2003-01-01

    Full Text Available We examined the genetic structure and the effects of a bottleneck in populations of the water rat Nectomys squamipes, a primary host of Schistosoma mansoni. Eight microsatellite loci were studied in 7 populations from the Sumidouro region of the Brazilian state of Rio de Janeiro. Our data, covering a four-year period during which a bottleneck occurred, revealed substantial variation (6-31 alleles per locus and high levels of both observed (0.718-0.789 and expected (0.748-0.832 heterozygosity. Most populations were in Hardy-Weinberg equilibrium without linkage disequilibrium between loci. Overall average genetic differentiation between populations (estimated with the F ST (q and R ST (r analogues was 0.037 for q and 0.060 for r. There was significant allelic and genotypic differentiation between populations, especially in pairwise comparisons that included the most geographically isolated population. Direct migration estimates showed a low rate of migration, indicating that infected N. squamipes populations had a limited ability to spread S. mansoni. When the pre- and post-bottleneck populations were compared there was no detectable reduction in heterozygosity or allele number, although a significant excess of heterozygosity was detected in the post-bottleneck population.

  5. Lack of association between alopecia areata and HLA class I and II in a southeastern Brazilian population*

    Science.gov (United States)

    Barbosa, Ângela Marques; Prestes-Carneiro, Luiz Euribel; Sobral, Aldri Roberta Sodoschi; Sakiyama, Marcelo Jun; Lemos, Bruna Cerávolo; de Abreu, Marilda Aparecida Milanez Morgado; Martos, Luciana Leite Crivelin; Moliterno, Ricardo Alberto

    2016-01-01

    Background Alopecia areata (AA) is a common disorder of unknown etiology that affects approximately 0.7% to 3.8% of patients among the general population. Currently, genetic and autoimmune factors are emphasized as etiopathogenic. Studies linking Human Leukocyte Antigens (HLA) to AA have suggested that immunogenetic factors may play a role in the disease's onset/development. Objectives To investigate an association between AA and HLA class I/II in white Brazilians. Methods: Patients and control groups comprised 33 and 112 individuals, respectively. DNA extraction was performed by column method with BioPur kit. Allele's classification was undertaken using the PCR-SSO technique. HLA frequencies were obtained through direct counting and subjected to comparison by means of the chi-square test. Results Most patients were aged over 16, with no familial history, and developed partial AA, with no recurrent episodes. Patients showed a higher frequency of HLA-B*40, HLA-B*45, HLA-B*53 and HLA-C*04 compared with controls, although P was not significant after Bonferroni correction. Regarding HLA class II, only HLA-DRB1*07 revealed statistical significance; nevertheless, it featured more prominently in controls than patients (P=0.04; Pc=0.52; OR=0.29; 95%; CI=0.07 to 1.25). P was not significant after Bonferroni correction. Conclusions The development of AA does not seem to be associated with HLA in white Brazilians, nor with susceptibility or resistance. The studies were carried out in populations with little or no miscegenation, unlike the Brazilian population in general, which could explain the inconsistency found. PMID:27438193

  6. Different risk factors influence peptic ulcer disease development in a Brazilian population

    Institute of Scientific and Technical Information of China (English)

    Rodrigo Buzinaro Suzuki; Rodrigo Faria Cola; Larissa Tranquilino Bardela Cola; Camila Garcia Ferrari; Fred Ellinger; Altino Luiz Therezo; Luis Carlos da Silva

    2012-01-01

    AIM:To investigate age,sex,histopathology and Helicobacter pylori (H.pylori) status,as risk factors for gastroduodenal disease outcome in Brazilian dyspeptic patients.METHODS:From all 1466 consecutive dyspeptic patients submitted to upper gastroscopy at Hospital das Clinicas of Marilia,antral biopsy specimens were obtained and subjected to histopathology and H.pylori diagnosis.All patients presenting chronic gastritis (CG)and peptic ulcer (PU) disease localized in the stomach,gastric ulcer (GU) and/or duodenal ulcer (DU) were included in the study.Gastric biopsies (n =668) positive for H.pylori by rapid urease test were investigated for vacuolating cytotoxin A (vacA) medium (m) region mosaicism by polymerase chain reaction.Logistic regression analysis was performed to verify the association of age,sex,histopathologic alterations,H.pylori diagnosis and vacA m region mosaicism with the incidence of DU,GU and CG in patients.RESULTS:Of 1466 patients submitted to endoscopy,1060 (72.3%) presented CG [male/female =506/554;mean age (year) ± SD =51.2 ± 17.81],88 (6.0%)presented DU [male/female =54/34; mean age (year)± SD =51.4 ± 17.14],and 75 (5.1%) presented GU[male/female =54/21; mean age (year) ± SD =51.3± 17.12] and were included in the comparative analysis.Sex and age showed no detectable effect on CG incidence (overall x2 =2.1,P =0.3423).Sex [Odds ratios (OR) =1.8631,P =0.0058] but not age (OR =0.9929,P =0.2699) was associated with DU and both parameters had a highly significant effect on GU (overall x2 =30.5,P < 0.0001).The histopathological results showed a significant contribution of ageing for both atrophy (OR =1.0297,P < 0.0001) and intestinal metaplasia (OR =1.0520,P < 0.0001).Presence of H.pylori was significantly associated with decreasing age (OR =0.9827,P < 0.0001) and with the incidence of DU (OR =3.6077,P < 0.0001).The prevalence of ml in DU was statistically significant (OR =2.3563,P =0.0018) but not in CG (OR =2.678,P =0.0863) and

  7. Headache complaints associated with psychiatric comorbidity in a population-based sample

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    Benseñor I.M.

    2003-01-01

    Full Text Available The objective of the present study was to determine the frequency at which people complain of any type of headache, and its relationship with sociodemographic characteristics and psychiatric comorbidity in São Paulo, Brazil. A three-step cluster sampling method was used to select 1,464 subjects aged 18 years or older. They were mainly from families of middle and upper socioeconomic levels living in the catchment area of Instituto de Psiquiatria. However, this area also contains some slums and shantytowns. The subjects were interviewed using the Brazilian version of the Composite International Diagnostic Interview version 1.1. (CIDI 1.1 by a lay trained interviewer. Answers to CIDI 1.1 questions allowed us to classify people according to their psychiatric condition and their headaches based on their own ideas about the nature of their illness. The lifetime prevalence of "a lot of problems with" headache was 37.4% (76.2% of which were attributed to use of medicines, drugs/alcohol, physical illness or trauma, and 23.8% attributed to nervousness, tension or mental illness. The odds ratio (OR for headache among participants with "nervousness, tension or mental illness" was elevated for depressive episodes (OR, 2.1; 95%CI, 1.4-3.4, dysthymia (OR, 3.4; 95%CI, 1.6-7.4 and generalized anxiety disorder (OR, 4.3; 95%CI, 2.1-8.6, when compared with patients without headache. For "a lot of problems with" headaches attributed to medicines, drugs/alcohol, physical illness or trauma, the risk was also increased for dysthymia but not for generalized anxiety disorder. These data show a high association between headache and chronic psychiatric disorders in this Brazilian population sample.

  8. Headache complaints associated with psychiatric comorbidity in a population-based sample.

    Science.gov (United States)

    Benseñor, I M; Tófoli, L F; Andrade, L

    2003-10-01

    The objective of the present study was to determine the frequency at which people complain of any type of headache, and its relationship with sociodemographic characteristics and psychiatric comorbidity in S o Paulo, Brazil. A three-step cluster sampling method was used to select 1,464 subjects aged 18 years or older. They were mainly from families of middle and upper socioeconomic levels living in the catchment area of Instituto de Psiquiatria. However, this area also contains some slums and shantytowns. The subjects were interviewed using the Brazilian version of the Composite International Diagnostic Interview version 1.1. (CIDI 1.1) by a lay trained interviewer. Answers to CIDI 1.1 questions allowed us to classify people according to their psychiatric condition and their headaches based on their own ideas about the nature of their illness. The lifetime prevalence of "a lot of problems with" headache was 37.4% (76.2% of which were attributed to use of medicines, drugs/alcohol, physical illness or trauma, and 23.8% attributed to nervousness, tension or mental illness). The odds ratio (OR) for headache among participants with "nervousness, tension or mental illness" was elevated for depressive episodes (OR, 2.1; 95%CI, 1.4-3.4), dysthymia (OR, 3.4; 95%CI, 1.6-7.4) and generalized anxiety disorder (OR, 4.3; 95%CI, 2.1-8.6), when compared with patients without headache. For "a lot of problems with" headaches attributed to medicines, drugs/alcohol, physical illness or trauma, the risk was also increased for dysthymia but not for generalized anxiety disorder. These data show a high association between headache and chronic psychiatric disorders in this Brazilian population sample.

  9. Sample size for monitoring sirex populations and their natural enemies

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    Susete do Rocio Chiarello Penteado

    2016-09-01

    Full Text Available The woodwasp Sirex noctilio Fabricius (Hymenoptera: Siricidae was introduced in Brazil in 1988 and became the main pest in pine plantations. It has spread to about 1.000.000 ha, at different population levels, in the states of Rio Grande do Sul, Santa Catarina, Paraná, São Paulo and Minas Gerais. Control is done mainly by using a nematode, Deladenus siricidicola Bedding (Nematoda: Neothylenchidae. The evaluation of the efficiency of natural enemies has been difficult because there are no appropriate sampling systems. This study tested a hierarchical sampling system to define the sample size to monitor the S. noctilio population and the efficiency of their natural enemies, which was found to be perfectly adequate.

  10. Assessing tiger population dynamics using photographic capture-recapture sampling

    Science.gov (United States)

    Karanth, K.U.; Nichols, J.D.; Kumar, N.S.; Hines, J.E.

    2006-01-01

    Although wide-ranging, elusive, large carnivore species, such as the tiger, are of scientific and conservation interest, rigorous inferences about their population dynamics are scarce because of methodological problems of sampling populations at the required spatial and temporal scales. We report the application of a rigorous, noninvasive method for assessing tiger population dynamics to test model-based predictions about population viability. We obtained photographic capture histories for 74 individual tigers during a nine-year study involving 5725 trap-nights of effort. These data were modeled under a likelihood-based, ?robust design? capture?recapture analytic framework. We explicitly modeled and estimated ecological parameters such as time-specific abundance, density, survival, recruitment, temporary emigration, and transience, using models that incorporated effects of factors such as individual heterogeneity, trap-response, and time on probabilities of photo-capturing tigers. The model estimated a random temporary emigration parameter of =K' =Y' 0.10 ? 0.069 (values are estimated mean ? SE). When scaled to an annual basis, tiger survival rates were estimated at S = 0.77 ? 0.051, and the estimated probability that a newly caught animal was a transient was = 0.18 ? 0.11. During the period when the sampled area was of constant size, the estimated population size Nt varied from 17 ? 1.7 to 31 ? 2.1 tigers, with a geometric mean rate of annual population change estimated as = 1.03 ? 0.020, representing a 3% annual increase. The estimated recruitment of new animals, Bt, varied from 0 ? 3.0 to 14 ? 2.9 tigers. Population density estimates, D, ranged from 7.33 ? 0.8 tigers/100 km2 to 21.73 ? 1.7 tigers/100 km2 during the study. Thus, despite substantial annual losses and temporal variation in recruitment, the tiger density remained at relatively high levels in Nagarahole. Our results are consistent with the hypothesis that protected wild tiger populations can remain

  11. High organochlorine accumulation in blubber of Guiana dolphin, Sotalia guianensis, from Brazilian coast and its use to establish geographical differences among populations

    Energy Technology Data Exchange (ETDEWEB)

    Lailson-Brito, J., E-mail: lailson@biof.ufrj.b [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil); Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ) (Brazil); Dorneles, P.R., E-mail: dorneles.paulo@uerj.b [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil); Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ) (Brazil); Azevedo-Silva, C.E., E-mail: ceass@biof.ufrj.b [Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ) (Brazil); Azevedo, A.F., E-mail: azevedo.alex@uerj.b [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil); Vidal, L.G. [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil); Zanelatto, R.C. [Pro- Reitoria de Administracao, Universidade Federal do Parana, Curitiba (Brazil); Lozinski, C.P.C. [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil); Azeredo, A., E-mail: aazeredo@uefs.b [Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ) (Brazil); Colegiado de Ciencias Farmaceuticas, Departamento de Saude, Universidade Estadual de Feira de Santana (UEFS) (Brazil); Fragoso, A.B.L. [Laboratorio de Mamiferos Aquaticos e Bioindicadores ' Prof. Izabel Gurgel' (MAQUA), Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro (UERJ) (Brazil)

    2010-05-15

    Blubber samples from 33 Guiana dolphins (Sotalia guianensis) from three estuaries (Guanabara, Sepetiba/Ilha Grande and Paranagua Bays) of Southern and Southeastern Brazil were analyzed for organochlorine compounds (DDTs, PCBs and HCB). The sampled individuals were incidentally captured in gillnet fishery between 1995 and 2005. The concentrations (in ng/g lipids) varied from 652 to 23 555 for SIGMADDT; from 765 to 99 175 for SIGMAPCB; and from <4.4 to 156 for HCB. The results have shown that cetaceans from Brazil present organochlorine concentrations that are comparable to those reported for highly industrialized regions of Northern Hemisphere. Using discriminant analysis it was possible to verify that the dolphin populations from the three bays present different organochlorine accumulation patterns. This feature allows the use of this set of pollutants as an auxiliary tool for identification of different populations of the species off Brazilian Coast. - The site fidelity of Guiana dolphins inhabiting polluted environments allows population differentiation through discriminant analysis using organochlorine profiles.

  12. Whole mitochondrial genome genetic diversity in an Estonian population sample.

    Science.gov (United States)

    Stoljarova, Monika; King, Jonathan L; Takahashi, Maiko; Aaspõllu, Anu; Budowle, Bruce

    2016-01-01

    Mitochondrial DNA is a useful marker for population studies, human identification, and forensic analysis. Commonly used hypervariable regions I and II (HVI/HVII) were reported to contain as little as 25% of mitochondrial DNA variants and therefore the majority of power of discrimination of mitochondrial DNA resides in the coding region. Massively parallel sequencing technology enables entire mitochondrial genome sequencing. In this study, buccal swabs were collected from 114 unrelated Estonians and whole mitochondrial genome sequences were generated using the Illumina MiSeq system. The results are concordant with previous mtDNA control region reports of high haplogroup HV and U frequencies (47.4 and 23.7% in this study, respectively) in the Estonian population. One sample with the Northern Asian haplogroup D was detected. The genetic diversity of the Estonian population sample was estimated to be 99.67 and 95.85%, for mtGenome and HVI/HVII data, respectively. The random match probability for mtGenome data was 1.20 versus 4.99% for HVI/HVII. The nucleotide mean pairwise difference was 27 ± 11 for mtGenome and 7 ± 3 for HVI/HVII data. These data describe the genetic diversity of the Estonian population sample and emphasize the power of discrimination of the entire mitochondrial genome over the hypervariable regions.

  13. BDNF gene polymorphism, cognition and symptom severity in a Brazilian population-based sample of first-episode psychosis subjects Polimorfismo do gene do BDNF, cognição e gravidade dos sintomas em uma amostra de base populacional brasileira de indivíduos apresentando o primeiro episódio psicótico

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    Eduardo Martinho Jr

    2012-10-01

    Full Text Available OBJECTIVE: To investigate the influence of brain-derived neurotrophic factor (BDNF gene variations on cognitive performance and clinical symptomatology in first-episode psychosis (FEP. METHODS: We performed BDNF val66met variant genotyping, cognitive testing (verbal fluency and digit spans and assessments of symptom severity (as assessed with the PANSS in a population-based sample of FEP patients (77 with schizophreniform psychosis and 53 with affective psychoses and 191 neighboring healthy controls. RESULTS: There was no difference in the proportion of Met allele carriers between FEP patients and controls, and no significant influence of BDNF genotype on cognitive test scores in either of the psychosis groups. A decreased severity of negative symptoms was found in FEP subjects that carried a Met allele, and this finding reached significance for the subgroup with affective psychoses (p OBJETIVO: Investigar a influência da variação do gene do fator neurotrófico derivado do cérebro (BDNF no desempenho cognitivo e na sintomatologia clínica durante o primeiro episódio psicótico (PEP. MÉTODOS: Foram realizados a genotipificação das variantes Val66met do BDNF, o teste cognitivo (fluência verbal e repetição de dígitos e as avaliações da gravidade dos sintomas (conforme avaliado pela Positive and Negative Syndrome Scale [PANSS] em uma amostra de pacientes com PEP de base populacional (77 com psicose esquizofreniforme e 53 com psicose afetiva e 191 vizinhos controle saudáveis. RESULTADOS: Não houve diferença na proporção de portadores do alelo Met entre pacientes com PEP e o grupo controle. Não houve influência significativa do genótipo do BDNF sobre a pontuação de cada um dos grupos psicóticos. Foi encontrada uma diminuição da gravidade dos sintomas negativos em sujeitos com PEP portadores do alelo Met, e essa descoberta mostrou-se significativa para o subgrupo com psicose afetiva (p < 0,01, ANOVA. CONCLUSÕES: Os

  14. Is it safe the empirical distal femoral resection angle of 5 to 6 of valgus in the Brazilian geriatric population?

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    Fernando Cury Rezende

    2013-09-01

    Full Text Available OBJECTIVE: The purpose of this study is to determine if there is a safe distal femoral resection angle to restore the normal axial alignment of the limb in total knee arthroplasty (TKA in the Brazilian geriatric population with knee arthrosis. METHOD: This study analyzed 99 pre-operative hip-knee-ankle radiographs of osteoarthritic knees of 66 patients (54 women, 12 men with knee osteoarthritis. The distal femoral cut angle was determined based on the femoral mechanical-anatomical angle (FMA. Mean, median and standard deviation measurements of the distal femoral cut angle were calculated, differentiated by gender and side. The mean result of the distal femoral resection angle was compared to 5.7°, the mean average angle of previous and similar study based on European population of patients with knee arthrosis. RESULTS: The mean average of the distal femoral resection angle of the study was 6.05 (range 3-9°. The distribution of this angle between genders showed a slight superior average of the male population (6.17° compared to the female (6.02°, but with no statistically significant difference (p = 0.726. There was no statistically significant difference (p = 0.052 between the mean average of this study (6.05° compared to the mean average of the literature (5.7°. However, considering 3° as the limit of acceptable error in the coronal plane, this empirical femoral resection angle would not be appropriated for 19.7% of the population. CONCLUSION: The distal femoral resection angle of 5-6° is not completely safe for the Brazilian geriatric population.

  15. Stellar populations in a complete sample of local radio galaxies

    CERN Document Server

    Raimann, D; Quintana, H; Hunstead, R; Wisotzki, L

    2005-01-01

    We investigate the nature of the continuum emission and stellar populations in the inner 1-3 kiloparsecs of a complete sample of twenty-four southern radio galaxies, and compare the results with a control sample of eighteen non-active early-type galaxies. Twelve of the radio galaxies are classified as Fanaroff-Riley type I (FRI), eight as FRII and four as intermediate or undefined type (FRx). Optical long-slit spectra are used to perform spectral synthesis as a function of distance from the nucleus at an average sampling of 0.5-1.0kpc and quantify the relative contributions of a blue featureless continuum and stellar population components of different ages. Our main finding is a systematic difference between the stellar populations of the radio and control sample galaxies: the former have a larger contribution from an intermediate age (1Gyr) component, suggesting a connection between the present radio activity and a starburst which occurred about 1Gyr ago. In addition, we find a correlation between the contri...

  16. Yfiler(Ⓡ) Plus population samples and dilution series

    DEFF Research Database (Denmark)

    Andersen, Mikkel Meyer; Mogensen, Helle Smidt; Eriksen, Poul Svante

    2017-01-01

    of crime scene samples were analysed with the customised thresholds and artefact filters. The Yfiler (Ⓡ) Plus Amplification Kit was sensitive giving full profiles down to 70 pg of male DNA. The balances between the fluorescent dyes as well as between loci were very good. The kit was able to produce full Y......-STR profiles from crime scene samples containing small amounts of male DNA and large amounts of female DNA (although unspecific reactions were evident for very unbalanced mixtures). A decrease in the drop-out rate was found for both the dilution series and population samples, as well as a small increase...... DNA complicated the analysis by causing drop-ins of characteristic female DNA artefacts. Even though the customised analytical threshold in combination with the custom-made artefact filters gave more alleles, crime scene samples still needed special attention from the forensic geneticist....

  17. [Poliomyelitis surveillance: seroepidemiologic study on a Piedmont population sample].

    Science.gov (United States)

    Maiello, A; Ossola, O; Guidetti, A; Zotti, C; Moiraghi Ruggenini, A

    1995-01-01

    In order to assess the immunity/receptivity towards the three poliovirus strains, a sample representative of the sex and age composition of the resident population in Piedmont at census 1981, was examined. For each subject data were collected in order to identify the population from social-ambient point of view. For the evaluation of antibodie's title to poliovirus 1, 2, 3, the serums were analyzed with the neutralization method using the microtitolation plates and epithelial larynx cancer cells (HEp-2), like revealing system. The samples with > or = 2 title were considered positives for specific antibodies. To determine the relation between presence/absence of specific poliovirus antibodies and the other variables, a multiple logistic regression was fitted and the odds ratio was calculated. The results of our study show an immunity in all age groups, underlying a herd immunity condition. Furthermore the incomplete antibodies response to the three poliovirus strains seems to be influenced by age only.

  18. Comportamento relativo à exposição e proteção solar na população de 15 anos ou mais de 15 capitais brasileiras e Distrito Federal, 2002-2003 Behaviors related to sunlight exposure versus protection in a random population sample from 15 Brazilian State capitals and the Federal District, 2002-2003

    Directory of Open Access Journals (Sweden)

    André Salem Szklo

    2007-04-01

    Full Text Available Este artigo explora a relação entre variáveis sócio-demográficas e comportamentais com proteção solar, levando-se em consideração as diferenças regionais existentes. Um inquérito de base populacional foi realizado em 15 capitais brasileiras e Distrito Federal, e as informações sobre exposição solar foram coletadas para um total de 16.999 indivíduos de 15 anos ou mais. Quando comparamos os indivíduos residentes nas regiões Norte e Sul do país, as diferenças brutas entre mulheres e homens nas proporções de utilização do protetor solar e do chapéu foram, respectivamente, para a Região Norte, +10,9% (IC95%: 7,1; 14,6 e -11,6% (IC95%: -17,0; -6,3, e Sul do país, +21,3% (IC95%: 17,7; 24,9 e -16,0% (IC95%: -20,2; -12,5. As diferenças ajustadas confirmaram que, tanto no Norte quanto no Sul do país, as mulheres mais freqüentemente utilizaram como proteção o filtro solar e menos freqüentemente referiram o uso de chapéu do que os homens. Contudo, essas diferenças não foram homogêneas entre as re- giões (termos de interação p This article examines region-specific relations between prevalence of protection against sunlight and socio-demographic and behavioral variables in Brazil. Data were derived from a cross-sectional population-based random sample. Information on sunlight exposure was available for a total of 16,999 individuals 15 years and older. Comparing the North and South of Brazil, crude differences between women and men in the use of "sunscreen" and "protective headwear" were +10.9% (95%CI: 7.1; 14.6 and -11.6% (95%CI: -17.0; -6.3 in the North and +21.3% (95%CI: 17.7; 24.9 and -16.0% (95%CI: -20.2; -12.5 in the South. Adjusted differences by selected variables confirmed that women use more sunscreen protection and less headwear protection as compared to men in both the North and South, but the difference was not homogeneous by region (interaction term p value < 0.05.

  19. Validation of a subjective well-being inventory with youth and adolescents Argentinean, Mexican, and Brazilian Samples

    Directory of Open Access Journals (Sweden)

    del Pino Peña, Roberto

    2009-12-01

    Full Text Available The aim of this study was to adapt the Well-Being Inventory of Nacpal & Shell for using with Latinamerican youth and adolescents. The conceptual, semantic and operational equivalence of inventory prototype version was analized. The final version was administered to a sample of 1,891 students (730 Mexicans, 664 Argentines and 497 Brazilians who also filled a set of recognized instruments developed to explore resilience, coping, sense of humor, emotional intelligence and cultural values. The exploration of factorial validity showed six orthogonal factors which explained almost 59% of total variance. Construct validity was demostrated through positive and significant correlations between subjective well-being and most of the variables considered. Reliability of the adapted instrument reached satisfactory levels. In light of the results some suggestions for future research in the area are made

  20. Emotional and sexual jealousy as a function of sex and sexual orientation in a Brazilian sample.

    Science.gov (United States)

    de Souza, Altay Alves Lino; Verderane, Michele Pereira; Taira, Juliana Tieme; Otta, Emma

    2006-04-01

    The goal of the present study was to compare the relative distress of homosexual and heterosexual Brazilian men and women on scenarios in which they imagined their partners sexually or emotionally involved with another person, using a forced-choice paradigm and continuous measures. Participants were 68 heterosexual men, 72 heterosexual women, 42 homosexual men, and 35 homosexual women. On the forced-choice questions heterosexual men (39 on one question and 37 on the other) were more upset than their female counterparts (21 on one question and 15 on the other) by scenarios of sexual infidelity than those of emotional infidelity. On questions using continuous measures no significant difference was found between pleasurable sex and attachment scenarios for heterosexual women or heterosexual men. On the highly upsetting scenarios heterosexual men discriminated between flirting and both pleasurable sex and attachment scenarios, being less disturbed by the former. In contrast, heterosexual women were equally distressed by the three scenarios. Scores for the homosexual men and homosexual women fell in between those of the heterosexual men and heterosexual women and did not show a clear cut preference for the sexual infidelity or the emotional alternative on the forced-choice paradigm. However, on the continuous measures of jealousy homosexuals resembled heterosexuals of the opposite sex. There was no evidence that jealousy would be less intense among homosexuals although reproductive outcomes were not at risk.

  1. Frontal assessment battery in a Brazilian sample of healthy controls: normative data

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    Rogério Beato

    2012-04-01

    Full Text Available OBJECTIVE: To show data on the performance of healthy subjects in the Frontal Assessment Battery (FAB, correlating with gender, age, education, and scores in the Mini-Mental State Examination (MMSE. METHODS: Two hundred and seventy-five healthy individuals with mean age of 66.4±10.6 years-old were evaluated. Mean total FAB scores were established according to the educational level. RESULTS: Mean total FAB scores according to the educational level were 10.9±2.3, for one to three years; 12.8±2.7, for four to seven years; 13.8±2.2, for eight to 11 years; and 15.3±2.3, for 12 or more years. Total FAB scores correlated significantly with education (r=0.47; p<0.0001 and MMSE scores (r=0.39; p<0.0001. No correlation emerged between FAB scores, age, and gender. CONCLUSION: In this group of healthy subjects, the Brazilian version of the FAB proved to be influenced by the education level, but not by age and gender.

  2. Haplotype diversity of 17 Y-str loci in an admixed population from the Brazilian Amazon

    Science.gov (United States)

    Francez, Pablo Abdon da Costa; Ramos, Luiz Patrick Vidal; de Jesus Brabo Ferreira Palha, Teresinha; dos Santos, Sidney Emanuel Batista

    2012-01-01

    The allelic and haplotype frequencies of 17 Y-STR loci most commonly used in forensic testing were estimated in a sample of 138 unrelated healthy males from Macapá, in the northern Amazon region of Brazil. The average gene diversity was 0.6554 ± 0.3315. 134 haplotypes of the 17 loci were observed, 130 of them unique and four present in two individuals each. The haplotype diversity index was 0.9996 + 0.0009, with the most frequent haplogroups being R1b (52.2%), E1b1b (11.6%), J2 (10.1%) and Q (7.2%). Most haplogroups of this population belonged to European male lineages (89.2%), followed by Amerindian (7.2%) and African (3.6%) lineages. PMID:22481873

  3. Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Jaqueline Gusmão

    2005-03-01

    Full Text Available Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the studied area. In contrast, F ST values found for Farfantepenaeus sp. and F. paulensis indicate that the populations of those two species are genetically structured, comprising different fishery stocks. The largest genetic differences in F. paulensis were found between Lagoa dos Patos (South and the two populations from Southeast Brazil. In Farfantepenaeus sp., significant differences were detected between the population from Recife and those from Fortaleza and Ilhéus.

  4. Fitness evaluation of two Brazilian Aedes aegypti field populations with distinct levels of resistance to the organophosphate temephos

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    Thiago Affonso Belinato

    2012-11-01

    Full Text Available In Brazil, decades of dengue vector control using organophosphates and pyrethroids have led to dissemination of resistance. Although these insecticides have been employed for decades against Aedes aegypti in the country, knowledge of the impact of temephos resistance on vector viability is limited. We evaluated several fitness parameters in two Brazilian Ae. aegypti populations, both classified as deltamethrin resistant but with distinct resistant ratios (RR for temephos. The insecticide-susceptible Rockefeller strain was used as an experimental control. The population presenting the higher temephos resistance level, Aparecida de Goiânia, state of Goiás (RR95 of 19.2, exhibited deficiency in the following four parameters: blood meal acceptance, amount of ingested blood, number of eggs and frequency of inseminated females. Mosquitoes from Boa Vista, state of Roraima, the population with lower temephos resistance level (RR95 of 7.4, presented impairment in only two parameters, blood meal acceptance and frequency of inseminated females. These results indicate that the overall fitness handicap was proportional to temephos resistance levels. However, it is unlikely that these disabilities can be attributed solely to temephos resistance, since both populations are also resistant to deltamethrin and harbour the kdr allele, which indicates resistance to pyrethroids. The effects of reduced fitness in resistant populations are discussed.

  5. Resistance Status to the Insecticides Temephos, Deltamethrin, and Diflubenzuron in Brazilian Aedes aegypti Populations

    Science.gov (United States)

    Viana-Medeiros, Priscila Fernandes; Araújo, Simone Costa; Martins, Ademir J.; Lima, José Bento Pereira

    2016-01-01

    Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethrin (PY), and diflubenzuron (IGR) of A. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. High levels of resistance to neurotoxic insecticides were detected in almost all populations: RR95 to temephos varied between 4.0 and 27.1; the lowest RR95 to deltamethrin was 13.1, and values higher than 70.0 were found. In contrast, all samples were susceptible to diflubenzuron (RR95 < 2.3). Biochemical tests performed with larvae and adults discarded the participation of acetylcholinesterase, the OP target, and confirmed involvement of the detoxifying enzymes esterases, mixed function oxidases, and glutathione-S-transferases. The results obtained were discussed taking into account the public chemical control component and the increase in the domestic use of insecticides during dengue epidemic seasons in the evaluated municipalities. PMID:27419140

  6. Resistance Status to the Insecticides Temephos, Deltamethrin, and Diflubenzuron in Brazilian Aedes aegypti Populations

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    Diogo Fernandes Bellinato

    2016-01-01

    Full Text Available Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP and pyrethroids (PY are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR are also employed in the control of larvae. We quantified resistance to temephos (OP, deltamethrin (PY, and diflubenzuron (IGR of A. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. High levels of resistance to neurotoxic insecticides were detected in almost all populations: RR95 to temephos varied between 4.0 and 27.1; the lowest RR95 to deltamethrin was 13.1, and values higher than 70.0 were found. In contrast, all samples were susceptible to diflubenzuron (RR95 < 2.3. Biochemical tests performed with larvae and adults discarded the participation of acetylcholinesterase, the OP target, and confirmed involvement of the detoxifying enzymes esterases, mixed function oxidases, and glutathione-S-transferases. The results obtained were discussed taking into account the public chemical control component and the increase in the domestic use of insecticides during dengue epidemic seasons in the evaluated municipalities.

  7. Chlamydia trachomatis infection in a sample of northern Brazilian pregnant women: prevalence and prenatal importance

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    Ana Paula B. de Borborema-Alfaia

    2013-10-01

    Full Text Available There are limited data regarding prevalence of Chlamydia trachomatis infection among northern Brazilian pregnant women. OBJECTIVE: The purpose of this study was to estimate the prevalence of chlamydial infection among pregnant women in their third trimester and to determine the repercussion of this infection on their offspring. METHODS: In the first phase of this study 100 pregnant women receiving prenatal care in a local public university hospital were examined to assess the prevalence of genital C. trachomatis infection by polymerase chain reaction technique. In the second phase, 88 pregnant women were prospectively evaluated for premature rupture of membranes, puerperal consequences associated with chlamydial infection, and neonates were checked for low-birth weight. RESULTS: The prevalence rate of chlamydial infection was 11%, and 72.7% of the positive participants were predominantly less than 30 years of age (p = 0.1319. A total of 36.4% of the participants had premature rupture of membranes (p = 0.9998. Neither low-birth weight infants nor preterm delivery were observed. A cohort of 16 newborn babies were followedup up to 60 days of life to ascertain outcome: 50% had respiratory symptoms. Neonates born to infected mothers had a higher risk to develop respiratory symptoms in the first 60 days of life. CONCLUSION: The scarcity of data about the effects of chlamydial infection on pregnancy and neonatal outcomes justified this study. Diagnosing and treating chlamydial infection during the third trimester of pregnancy may prevent neonate infection. Therefore, preventive screening should be seen as a priority for early detection of asymptomatic C. trachomatis infection as part of local public health strategies.

  8. Identifying student profiles and their impact on academic performance in a Brazilian undergraduate student sample.

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    da Silva, E T; de Fátima Nunes, M; Santos, L B; Queiroz, M G; Leles, C R

    2012-02-01

    The recognition of the student profile provides strategic information for planning educational policies in the university environment. The aims of this study were to identify natural segmentation of freshman undergraduate dental students based on demographic, socioeconomic and educational variables, and to subsequently investigate their impact on academic performance of Brazilian undergraduate students. Cluster analysis (two-step algorithm) was used to segment students who entered dental school in the time period from 1999 to 2001 (n=158) into groups based on responses to a questionnaire completed by students at the time of the admission examination. Clustering analysis revealed three natural groups. Age, the parents' level of education, and performance on the first admission test were the most important variables for cluster segmentation. Cluster 1 (n=42; 26.6%) was characterized by female students with higher socioeconomic status and better previous educational indicators. Cluster 2 (n=62; 39.2%) represented disadvantaged socioeconomic profiles, with a predominance of females and older students. Cluster 3 (n=54; 34.2%) showed similar socioeconomic characteristics to cluster 1, except for male prevalence, higher age, and experiencing difficulty in the admission test. Clusters' academic performance was satisfactory in both overall course and course groups (grade point average of at least 7.0), with average ranging from 7.89 (SD=0.44) to 8.13 (SD=0.31) and 7.37 (SD=0.75) to 8.31(SD=0.26), respectively. Our findings provide encouraging evidence for the current context of equality of access to education and reveal the importance of financial support to maximize successful educational experiences of socioeconomically disadvantaged dental students.

  9. Lack of Association between a 3'UTR VNTR Polymorphism of Dopamine Transporter Gene (SLC6A3) and ADHD in a Brazilian Sample of Adult Patients

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    Aperecida da Silva, Maria; Cordeiro, Quirino; Louza, Mario; Vallada, Homero

    2011-01-01

    Objective: To investigate a possible association between a 3'UTR VNTR polymorphism of the dopamine transporter gene (SLC6A3) and ADHD in a Brazilian sample of adult patients. Method: Study Case-control with 102 ADHD adult outpatients ("DSM-IV" criteria) and 479 healthy controls. The primers' sequence used were: 3'UTR-Forward: 5' TGT GGT…

  10. Lack of Association between a 3'UTR VNTR Polymorphism of Dopamine Transporter Gene (SLC6A3) and ADHD in a Brazilian Sample of Adult Patients

    Science.gov (United States)

    Aperecida da Silva, Maria; Cordeiro, Quirino; Louza, Mario; Vallada, Homero

    2011-01-01

    Objective: To investigate a possible association between a 3'UTR VNTR polymorphism of the dopamine transporter gene (SLC6A3) and ADHD in a Brazilian sample of adult patients. Method: Study Case-control with 102 ADHD adult outpatients ("DSM-IV" criteria) and 479 healthy controls. The primers' sequence used were: 3'UTR-Forward: 5' TGT GGT GAT GGG…

  11. Comparative Estimation of Genetic Diversity in Population Studies using Molecular Sampling and Traditional Sampling Methods.

    Science.gov (United States)

    Saeb, Amr Tm; David, Satish Kumar

    2014-01-01

    Entomopathogenic nematodes (EPN) are efficient biological pest control agents. Population genetics studies on EPN are seldom known. Therefore, it is of interest to evaluate the significance of molecular sampling method (MSM) for accuracy, time needed, and cost effectiveness over traditional sampling method (TSM). The study was conducted at the Mohican Hills golf course at the state of Ohio where the EPN H. bacteriophora has been monitored for 18 years. The nematode population occupies an area of approximately 3700 m(2) with density range from 0.25-2 per gram soil. Genetic diversity of EPN was studied by molecular sampling method (MSM) and traditional sampling method (TSM) using the mitochondrial gene pcox1. The MSM picked 88% in compared to TSM with only 30% of sequenced cox 1 gene. All studied genetic polymorphism measures (sequence and haplotype) showed high levels of genetic diversity of MSM over TSM. MSM minimizes the chance of mitochondrial genes amplification from non target organisms (insect or other contaminating microorganisms). Moreover, it allows the sampling of more individuals with a reliable and credible representative sample size. Thus, we show that MSM supersedes TSM in labour intensity, time consumption and requirement of no special experience and efficiency.

  12. Polymorphic CAG and GGC repeat lengths in the androgen receptor gene and prostate cancer risk: analysis of a Brazilian population.

    Science.gov (United States)

    Silva Neto, Brasil; Koff, Walter J; Biolchi, Vanderlei; Brenner, Cleber; Biolo, Karlo D; Spritzer, Poli Mara; Brum, Ilma S

    2008-02-01

    Variations in transcriptional activity of the androgen receptor (AR) are related to polymorphic CAG and GGC repeats in exon 1 of the AR gene. We investigated the association between CAG and GGC repeat length and the risk of prostate cancer in a case-control study from a Brazilian population. We evaluated 49 patients and 51 healthy controls. DNA was extracted from peripheral leukocytes and the AR gene was analyzed by fragment analysis (GeneMapper software, Applied Biosystems, Foster City, California, USA). CAG and GGC mean lengths were not different between cases and controls. The risk for prostate cancer was higher for CAG repeats repeat lengths (CAG + GGC) repeats ( 17) were not associated with risk for prostate cancer (OR = 1.13 [95% CI 0.47-2.75]). In conclusion, fewer number of CAG repeats and total repeats (CAG + GGC) in the AR gene may be associated with increased risk for prostate cancer.

  13. The Impact of Selection with Diflubenzuron, a Chitin Synthesis Inhibitor, on the Fitness of Two Brazilian Aedes aegypti Field Populations.

    Science.gov (United States)

    Belinato, Thiago Affonso; Valle, Denise

    2015-01-01

    Several Aedes aegypti field populations are resistant to neurotoxic insecticides, mainly organophoshates and pyrethroids, which are extensively used as larvicides and adulticides, respectively. Diflubenzuron (DFB), a chitin synthesis inhibitor (CSI), was recently approved for use in drinking water, and is presently employed in Brazil for Ae. aegypti control, against populations resistant to the organophosphate temephos. However, tests of DFB efficacy against field Ae. aegypti populations are lacking. In addition, information regarding the dynamics of CSI resistance, and characterization of any potential fitness effects that may arise in conjunction with resistance are essential for new Ae. aegypti control strategies. Here, the efficacy of DFB was evaluated for two Brazilian Ae. aegypti populations known to be resistant to both temephos and the pyrethroid deltamethrin. Laboratory selection for DFB resistance was then performed over six or seven generations, using a fixed dose of insecticide that inhibited 80% of adult emergence in the first generation. The selection process was stopped when adult emergence in the diflubenzuron-treated groups was equivalent to that of the control groups, kept without insecticide. Diflubenzuron was effective against the two Ae. aegypti field populations evaluated, regardless of their resistance level to neurotoxic insecticides. However, only a few generations of DFB selection were sufficient to change the susceptible status of both populations to this compound. Several aspects of mosquito biology were affected in both selected populations, indicating that diflubenzuron resistance acquisition is associated with a fitness cost. We believe that these results can significantly contribute to the design of control strategies involving the use of insect growth regulators.

  14. Semi-volatile compounds variation among Brazilian populations of Ilex paraguariensis St. Hil.

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    Rogério Luis Cansian

    2008-02-01

    Full Text Available The use of compressed carbon towards extracting semi-volatile compounds present in maté leaves (Ilex paraguariensis St. Hil. is due to the growing interest in mate constituents to develop new products in the cosmetic, pharmaceutical and food industries. The objective of this work was to assess the chemical distribution of semi-volatile compounds in 20 native populations of maté collected all over Brazil. The extracts of bulk samples (30 plants of each population were obtained by the high-pressure carbon dioxide extraction technique, and analyzed by GC/MSD. The quantification of compounds (caffeine, theobromine, phytol, squalene, vitamin E, eicosane, pentatriacontane, and stigmasterol showed significant variations within the different populations and compounds analyzed, which are not related to geographical origin or macroclimate characteristics. The results pointed out to the importance of genetic and local environmental factors on the chemical composition of this species.A extração empregando dióxido de carbono a altas pressões em erva-mate (Ilex paraguariensis St. Hil., tem sido justificada pelo crescente interesse desta matriz vegetal ou de parte de seus constituintes na formulação de novos produtos, tais como cosméticos e medicamentos, entre outros. Neste contexto, o objetivo do presente trabalho é avaliar a distribuição química de compostos semi-voláteis em 20 populações de erva-mate coletadas em toda área de distribuição desta espécie no Brasil. 30 plantas foram selecionadas para produzir a amostra de cada população. Os extratos de cada amostra foram obtidos por extração com dióxido de carbono a alta pressão e, posteriormente, foram analisados por CG/EM. A quantificação de alguns compostos semi-voláteis presentes nos extratos (cafeína, teobromina, fitol, esqualeno, vitamina E, eicosano, pentatriacontano e stigmasterol, apresentaram variações significativas entre as concentrações dos diferentes compostos

  15. Built environment and social environment: associations with overweight and obesity in a sample of Brazilian adults

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    Gustavo Velásquez-Meléndez

    2013-10-01

    Full Text Available The aim of this study was to assess associations between the built environment and social environment and excess weight in an urban population. Participants were selected from the Surveillance System for Risk Factors for Chronic Diseases (VIGITEL. The study used data from the city of Belo Horizonte, Minas Gerais State, Brazil. A total of 3,425 interviews from the years 2008 and 2009 were used. Georeferenced data on parks, squares, and locations for physical exercise, population density, and food stores were used to assess the built environment. Description of the social environment used income and homicide rate for the neighborhood. Environmental variables associated independently with excess weight were population density, presence of parks, squares, and locations for physical exercise, and self-reported presence of locations for physical exercise. The findings show that residential neighborhood characteristics are associated with excess weight in urban adults.

  16. ANALYTICAL STUDY OF POPULATION STRUCTURE BY SAMPLING IN RURAL AREAS

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    F. Azordegan

    1973-03-01

    Full Text Available Despite the care taken to ensure the quality of date collected by enumeration and sampling, the final tabulation will sometimes give obvious indication of errors in basic information. Evaluation of these errors furnishes those who use such a study and sampling. More general, the finding in these studies should be helpful to others that conduct surveys. In order to compare the age composition of the rural areas between two provinces of IRAN, i.e. AZARBAIJAN, and ISFAHAN, a sample of each has selected (8 and 23 counties respectively.The main idea was to evaluate the errors in age recording and the effect of digit preference on the age composition of the population. It became evident that, AZARBAIJAN has a smaller family size, lower median age, higher sex ratio and lower dependency ratio than ISFAHAN. More over, the age pyramid of ISFAHAN has a better shape and more regular than AZARBAIJAN and therefore the Myer’s Index which determines the degree of dispersion in age reporting is lower in ISFAHAN which may be the result of higher literacy in ISFAHAN. Generally men in both samples report their age more precisely than women.

  17. Toward high-resolution population genomics using archaeological samples

    Science.gov (United States)

    Morozova, Irina; Flegontov, Pavel; Mikheyev, Alexander S.; Bruskin, Sergey; Asgharian, Hosseinali; Ponomarenko, Petr; Klyuchnikov, Vladimir; ArunKumar, GaneshPrasad; Prokhortchouk, Egor; Gankin, Yuriy; Rogaev, Evgeny; Nikolsky, Yuri; Baranova, Ancha; Elhaik, Eran; Tatarinova, Tatiana V.

    2016-01-01

    The term ‘ancient DNA’ (aDNA) is coming of age, with over 1,200 hits in the PubMed database, beginning in the early 1980s with the studies of ‘molecular paleontology’. Rooted in cloning and limited sequencing of DNA from ancient remains during the pre-PCR era, the field has made incredible progress since the introduction of PCR and next-generation sequencing. Over the last decade, aDNA analysis ushered in a new era in genomics and became the method of choice for reconstructing the history of organisms, their biogeography, and migration routes, with applications in evolutionary biology, population genetics, archaeogenetics, paleo-epidemiology, and many other areas. This change was brought by development of new strategies for coping with the challenges in studying aDNA due to damage and fragmentation, scarce samples, significant historical gaps, and limited applicability of population genetics methods. In this review, we describe the state-of-the-art achievements in aDNA studies, with particular focus on human evolution and demographic history. We present the current experimental and theoretical procedures for handling and analysing highly degraded aDNA. We also review the challenges in the rapidly growing field of ancient epigenomics. Advancement of aDNA tools and methods signifies a new era in population genetics and evolutionary medicine research. PMID:27436340

  18. [Design and implementation of the ELSA-Brasil biobank: a prospective study in a Brazilian population].

    Science.gov (United States)

    Pereira, Alexandre C; Bensenor, Isabela M; Fedeli, Ligia M; Castilhos, Cristina; Vidigal, Pedro G; Maniero, Viviane; Leite, Claudia M; Pimentel, Robercia A; Duncan, Bruce B; Mill, Jose Geraldo; Lotufo, Paulo A

    2013-06-01

    The Brazilian Longitudinal Study for Adult Health (ELSA-Brasil) is a multicenter prospective cohort of civil servants designed to assess the determinants of chronic diseases, especially cardiovascular diseases and type 2 diabetes. The present article describes the main design and implementation points of the ELSA-Brasil biobank project. Economic, political, logistical and technological aspects of this study are characterized. Additionally, it discusses the final biorepository protocol and the facilities implemented to achieve this objective. The design and implementation process of the ELSA-Brasil biobank took three years to be performed. Both the central and local biobanks were built according to the best biorepository techniques, using different technological solutions for the distinct needs expected in this study.

  19. Sampling sufficiency of the anatomical characteristics of Brazilian hardwood using the resampling method

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    André Luiz Missio

    2014-02-01

    Full Text Available Sampling sufficiency of the anatomical characteristics of fibres from Luehea divaricata wood was investigated through the bootstrap resampling method. Sampling sufficiency of fibre length, fibre diameter, lumen diameter and fibre wall thickness were determined. Three scenarios of sampling sufficiency evaluation were used: general, segregation between juvenile and mature wood, and accumulation throughout the life of the tree. The segregation of juvenile and mature wood showed that sampling sufficiency in juvenile wood was higher than in mature wood, up to three times for the fibre length. In general, results indicated higher values than suggested in specific standards and by other authors. Therefore, resampling bootstrap methodology, even though underused in forestry research, is an alternative for new studies, as it does not have restrictive hypotheses such as data normality.

  20. Characterization of Microbial Population Shifts During Sample Storage

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    Heath J. Mills

    2012-02-01

    Full Text Available The objective of this study was to determine shifts in the microbial community structure and potential function based on standard Integrated Ocean Drilling Program storage procedures for sediment cores. Standard long-term storage protocols maintain sediment temperature at 4oC for mineralogy, geochemical, and/or geotechnical analysis whereas standard microbiological sampling immediately preserves sediments at -80oC. Storage at 4oC does not take into account populations may remain active over geologic time scales at temperatures similar to storage conditions. Identification of active populations within the stored core would suggest geochemical and geophysical conditions within the core change over time. To test this potential, the metabolically active fraction of the total microbial community was characterized from IODP Expedition 325 Great Barrier Reef sediment cores prior to and following a three-month storage period. Total RNA was extracted from complementary 2, 20, and 40 m below seafloor sediment samples, reverse transcribed to cDNA and then sequenced using 454 FLX sequencing technology, yielding over 14,800 sequences from the six samples. Interestingly, 97.3% of the sequences detected were associated with lineages that changed in detection frequency during the storage period including key biogeochemically relevant lineages associated with nitrogen, iron and sulfur cycling. These lineages have the potential to permanently alter the physical and chemical characteristics of the sediment promoting misleading conclusions about the in situ biogeochemical environment. In addition, the detection of new lineages after storage increases the potential for a wider range of viable lineages within the subsurface that may be underestimated during standard community characterizations.

  1. Neuropsychological Profiles Correlated with Clinical and Behavioral Impairments in a Sample of Brazilian Children with Attention-Deficit Hyperactivity Disorder.

    Science.gov (United States)

    Rizzutti, Sueli; Schuch, Viviane; Augusto, Bruno Muszkat; Coimbra, Caio Colturato; Pereira, João Pedro Cabrera; Bueno, Orlando Francisco Amodeo

    2015-01-01

    Attention-deficit hyperactivity disorder (ADHD) is a complex neurodevelopmental disorder that implies several-step process, and there is no single test to diagnose both ADHD and associated comorbidities, such as oppositional-defiant disorder (ODD), anxiety disorder, depression, and certain types of learning disabilities. The purpose of the present study was to examine correlations between behavioral and clinical symptoms by administering an extensive neuropsychological battery to a sample of children and adolescents from a developing country. The sample was divided into three groups: non-ADHD, ADHD-non-comorbid, and ADHD + comorbidity. A full neuropsychological battery and clinical assessment found that 105 children met DSM-5 criteria, of whom 46.6% had the predominantly inattentive presentation, 37.3% had combined presentation, and 16% were predominantly hyperactive/impulsive presentation. The internal correlation between neuropsychological tests did not reach statistical significance in the comparison between ADHD and non-ADHD cases (p disorder showed close correlations between clinical CBCL profiles and externalized symptoms. Our findings suggest that ADHD + comorbidity and ADHD non-comorbid cases may be differentiated by a number of neuropsychological measures, such as processing speed, inhibitory control, and working memory, that may reflect different levels of involvement of the hot and cool executive domains, which are more impaired in cases of severe symptomatic-externalized behavior and emotional regulation problems. Therefore, profiles based on clinical and behavioral findings can help clinicians select better strategies for detecting neuropsychological impairment in Brazilian children with ADHD.

  2. Association study between the rs165599 catechol-O-methyltransferase genetic polymorphism and schizophrenia in a Brazilian sample

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    Quirino Cordeiro

    2012-12-01

    Full Text Available Schizophrenia is a severe psychiatric disorder with frequent recurrent psychotic relapses and progressive functional impairment. It results from a poorly understood gene-environment interaction. The gene encoding catechol-O-methyltransferase (COMT is a likely candidate for schizophrenia. Its rs165599 (A/G polymorphism has been shown to be associated with alteration of COMT gene expression. Therefore, the present study aimed to investigate a possible association between schizophrenia and this polymorphism. The distribution of the alleles and genotypes of this polymorphism was investigated in a Brazilian sample of 245 patients and 834 controls. The genotypic frequencies were in Hardy-Weinberg equilibrium and no statistically significant differences were found between cases and controls when analyzed according to gender or schizophrenia subtypes. There was also no difference in homozygosis between cases and controls. Thus, in the sample studied, there was no evidence of any association between schizophrenia and rs165599 (A/G polymorphism in the non-coding region 3' of the COMT gene.

  3. [Critical analysis of the diet quality of the Brazilian population according to the Healthy Eating Index: a systematic review].

    Science.gov (United States)

    Moreira, Patrícia Regina Silva; Rocha, Naruna Pereira; Milagres, Luana Cupertino; de Novaes, Juliana Farias

    2015-12-01

    In light of the importance of studying instruments that assess the food quality of the population, this study sought to conduct a systematic review of the quality of the diet of the Brazilian population using the Healthy Eating Index (HEI) and duly analyzing its methodology and results. The major electronic databases were used for the selection of studies. After the searches with the key words, 32 articles were included in this review. The growing interest of the scientific community in addressing this issue was observed, with recent studies using this instrument in Brazil. Methodological issues of articles were evaluated and discussed taking into consideration the revised versions and adaptations of the HEI. Some common results were highlighted among the studies such as low consumption of fruit, vegetables and/or dairy products, and the wider consumption of meat and eggs, cholesterol, total fat and saturated fat. Among the articles that address the HEI and socioeconomic aspects it was seen that quality of diet improves both in accordance with the increasing level of education of parents and with the family income in the population studied. The HEI can be used to monitor changes in dietary patterns and also as a nutrition education and health promotion tool.

  4. Seasonal Variation, Chemical Composition and Antioxidant Activity of Brazilian Propolis Samples

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    Érica Weinstein Teixeira

    2010-01-01

    Full Text Available Total phenolic contents, antioxidant activity and chemical composition of propolis samples from three localities of Minas Gerais state (southeast Brazil were determined. Total phenolic contents were determined by the Folin–Ciocalteau method, antioxidant activity was evaluated by DPPH, using BHT as reference, and chemical composition was analyzed by GC/MS. Propolis from Itapecerica and Paula Cândido municipalities were found to have high phenolic contents and pronounced antioxidant activity. From these extracts, 40 substances were identified, among them were simple phenylpropanoids, prenylated phenylpropanoids, sesqui- and diterpenoids. Quantitatively, the main constituent of both samples was allyl-3-prenylcinnamic acid. A sample from Virginópolis municipality had no detectable phenolic substances and contained mainly triterpenoids, the main constituents being α- and β-amyrins. Methanolic extracts from Itapecerica and Paula Cândido exhibited pronounced scavenging activity towards DPPH, indistinguishable from BHT activity. However, extracts from Virginópolis sample exhibited no antioxidant activity. Total phenolic substances, GC/MS analyses and antioxidant activity of samples from Itapecerica collected monthly over a period of 1 year revealed considerable variation. No correlation was observed between antioxidant activity and either total phenolic contents or contents of artepillin C and other phenolic substances, as assayed by CG/MS analysis.

  5. Pollen analysis in honey samples from the two main producing regions in the Brazilian northeast.

    Science.gov (United States)

    Sodré, Geni da S; Marchini, Luís C; Carvalho, Carlos A L de; Moreti, Augusta C de C C

    2007-09-01

    Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two states of the northeastern region of Brazil, Piauí (38 samples) and Ceará (20 samples), and to verify the potential of the honey plants during the months of February to August. The samples were obtained directly from beekeepers in each state and analyzed at the Apiculture Laboratory of the Entomology Section of Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, State of São Paulo, Brazil. The pollen analysis was performed using the acetolysis method. The samples were submitted to both a qualitative and a quantitative analysis. The dominant pollen in the State of Ceará is from Mimosa caesalpiniaefolia, M. verrucosa, Borreria verticillata, Serjania sp., and a Fabaceae pollen type, while in the State of Piauí it is from Piptadenia sp., M. caesalpiniaefolia, M. verrucosa, Croton urucurana and Tibouchina sp.

  6. Antigenic typing of brazilian rabies virus samples isolated from animals and humans, 1989-2000

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    FAVORETTO Silvana Regina

    2002-01-01

    Full Text Available Animal and human rabies samples isolated between 1989 and 2000 were typified by means of a monoclonal antibody panel against the viral nucleoprotein. The panel had been previously established to study the molecular epidemiology of rabies virus in the Americas. Samples were isolated in the Diagnostic Laboratory of the Pasteur Institute and in other rabies diagnostic centers in Brazil. In addition to the fixed virus samples CVS-31/96-IP, preserved in mouse brain, and PV-BHK/97, preserved in cell culture, a total of 330 rabies virus samples were isolated from dogs, cats, cattle, horses, bats, sheep, goat, swine, foxes, marmosets, coati and humans. Six antigenic variants that were compatible with the pre-established monoclonal antibodies panel were defined: numbers 2 (dog, 3 (Desmodus rotundus, 4 (Tadarida brasiliensis, 5 (vampire bat from Venezuela, 6 (Lasiurus cinereus and Lab (reacted to all used antibodies. Six unknown profiles, not compatible with the panel, were also found. Samples isolated from insectivore bats showed the greatest variability and the most commonly isolated variant was variant-3 (Desmodus rotundus. These findings may be related to the existence of multiple independent transmission cycles, involving different bat species.

  7. Alpha-synuclein A53T mutation is not frequent on a sample of Brazilian Parkinson’s disease patients

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    Gabriela S. Longo

    2015-06-01

    Full Text Available Introduction The pathogenesis of Parkinson’s disease (PD involves both genetic susceptibility and environmental factors, with focus on the mutation in the alpha-synuclein gene (SNCA.Objective To analyse the polymorphism SNCA-A53T in patients with familial PD (FPD and sporadic PD (SPD.Method A total of 294 individuals were studied, regardless of sex and with mixed ethnicity. The study group with 154 patients with PD, and the control group included 140 individuals without PD. The genotyping of SNCA-A53T was performed by PCR/RFLP. Significance level was p < 0.05.Results Among all patients, 37 (24% had FPD and 117 (75.9% had SPD. The absence of SNCA-A53T mutation was observed in all individuals.Conclusion SPD is notably observed in patients. However, the SNCA-A53T mutation was absent in all individuals, which does not differ controls from patients. This fact should be confirmed in a Brazilian study case with a more numerous and older population.

  8. FAS and FASL Gene Polymorphisms Are Not Associated with Hepatitis B Virus Infection Based on a Case-Control Study in a Brazilian Population

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    Bárbara B. Santana

    2013-01-01

    Full Text Available Objective. This study investigated the association of the single nucleotide polymorphisms (SNPs in the FAS and FASL genes with the outcome of hepatitis B virus (HBV infection. Methods. Blood samples were collected from 116 HBV-infected patients at the Hospital of the Santa Casa de Misericordia Foundation (Belém, PA, Brazil. Seronegative individuals were used as controls. DNA samples were extracted from the leukocytes and assayed using the polymerase chain reaction (PCR followed by RFLP analysis with restriction endonucleases. Results. The frequencies of the mutant genotypes for -670FAS (GG, Ivs2nt-124FASL (GG, Ivs3nt-169FASL (ΔT/ΔT, and -844FASL (TT were higher in the HBV patients, and the FAS-1377AA genotype was more frequent in the control group; however, the differences between the allele and genotype frequencies were not statistically significant. When the HBV patient population was divided into two groups (inactive carriers and active chronic hepatitis patients, the mutant genotypes were found to be more prevalent in the active chronic hepatitis group with respect to the FAS gene polymorphisms; however, this difference was not statistically significant. Conclusions. The results suggest that the polymorphisms in FAS and FASL genes are not associated with HBV infection or even with the natural history of the infection in the Brazilian Amazon region.

  9. Impact of Malocclusion on the Quality of Life of Brazilian Adolescents: A Population-Based Study.

    Science.gov (United States)

    Silva, Luciana Freitas Gomes E; Thomaz, Erika Bárbara Abreu Fonseca; Freitas, Heloiza Viana; Pereira, Alex Luiz Pozzobon; Ribeiro, Cecília Cláudia Costa; Alves, Cláudia Maria Coelho

    The objective of this study was to evaluate the impact of malocclusion on the quality of life (QOL) of adolescents in Brazil. We carried out a cross-sectional study in a sample population of 1015 schoolchildren aged 12 to 15 years from São Luís, Maranhão, Brazil. The explanatory variable was malocclusion, evaluated on the basis of the normative need or the adolescent's self-perceived need for dental treatment. Normative need for dental treatment was determined by professional diagnosis, made on the basis of Angle's classification, the Dental Aesthetic Index, and other morphological deviations (e.g., posterior crossbite, posterior open bite, and deep overbite). We analyzed the impact of malocclusion on the QOL using the Portuguese version of the Oral Health Impact Profile-14. Associations were estimated by using the prevalence ratio (PR) in Poisson regression analysis, with hierarchized modeling. An alpha of 5% was adopted as the criterion for statistical significance. The QOL of adolescents was impacted by malocclusion, classified by a normative need for treatment according to the Dental Aesthetic Index (PR = 1.27; 95% confidence interval [CI] = 1.03-1.56) or by the self-perceived need for treatment (PR = 2.54; 95% CI = 1.81-3.56). Certain sociodemographic variables, including the head of the family (PR = 1.52; 95% CI = 1.02-2.23), greater educational level of the head of the family (PR = 0.32; 95% CI = 0.17-0.61), and female sex (PR = 1.40; 95% CI = 1.05-1.89), had negative associations with QOL. We conclude that malocclusion has a negative impact on the QOL of adolescents, associated with socioeconomic conditions and the cosmetic effects of malocclusion.

  10. Fire effects on the population structure of Zanthoxylum rhoifolium Lam (Rutaceae) in a Brazilian savanna

    OpenAIRE

    IA. Silva; MW. Valenti; DM. Silva-Matos

    2009-01-01

    Since cerrado fires may impede the growth of seedlings into trees, they may shape the population of woody species. In this study, we assessed the effects of a severe fire on the population structure and spatial distribution of Zanthoxylum rhoifolium, a widespread cerrado tree. We were interested to know the importance of the resprouting and sexual reproduction in regenerating the population. The study area had been for about six years protected from fire, before a severe fire at the end of th...

  11. Spread of phosphine resistance among brazilian populations of three species of stored product insects.

    Science.gov (United States)

    Pimentel, Marco A G; Faroni, Lêda R D'A; Silva, Felipe H da; Batista, Maurílio D; Guedes, Raul N C

    2010-01-01

    The resistance to fumigant insecticides in stored-products insects is often recorded. Several factors influence the evolution of insecticide resistance. Among these, the frequency of applications and the migration of resistant populations are of primary importance for the stored-product insects. The aim of this study was to characterize the spectrum and investigate the status of phosphine resistance in Brazil, in 13 populations of the Coleoptera Tribolium castaneum Herbst (Tenebrionidae), ten populations of Rhyzopertha dominica (Fabr.) (Bostrichidae), and eight populations of Oryzaephilus surinamensis (L.) (Silvanidae). The pattern of resistance dispersion in the populations of these species was also verified. The bioassays for the detection of phosphine resistance followed the FAO standard method. To test the influence of migration in the evolution of the phosphine resistance, the difference of mortality in the discriminating concentration and the geographical distance among each pair wise combination of collection sites were correlated. None of the populations exhibited mortality above 90% in the discriminating concentration, for the three species. Mortality in the discriminating concentration increased with the geographical distance for R.dominica and O.surinamensis. However, no significant linear response was observed among the variables for T.castaneum populations. These results suggest that the dispersion of insects and the local selection are relevant in the evolution of the phosphine resistance in populations of R.dominica and O.surinamensis. In contrast, grain trade and local selection are probably the factors that determine the evolution of the phosphine resistance in populations of T. castaneum.

  12. Relationship of IL-1 and TNF-α polymorphisms with Helicobacter pylori in gastric diseases in a Brazilian population

    Energy Technology Data Exchange (ETDEWEB)

    Santos, J.C. [Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, SP (Brazil); Ladeira, M.S.P. [Departamento de Patologia, Universidade Estadual Paulista, Botucatu, SP (Brazil); Pedrazzoli, J. Jr.; Ribeiro, M.L. [Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, SP (Brazil)

    2012-06-22

    It is well known that the risk of development of gastric cancer (GC) in Helicobacter pylori-infected patients depends on several factors. Thus, the aim of this study was to investigate the effect of proinflammatory cytokine gene polymorphisms for IL-1β, IL-1RN and TNF-α on the development of GC in a Brazilian population. A total of 202 biopsies obtained from Brazilian patients with chronic gastritis and GC were included in the study. Infection with H. pylori cagA{sup +} was determined by the polymerase chain reaction (PCR) as previously described. IL-1β, IL-1RN and TNF-α polymorphism genotyping was performed by restriction fragment length polymorphism PCR. Associations between gene polymorphisms, clinical diseases and virulence markers were evaluated using either the X{sup 2} test or the Fisher exact test. Our results demonstrated that the IL-1β -511 C/C and IL-1β -511 C/T alleles were associated with chronic gastritis in H. pylori-positive patients (P = 0.04 and P = 0.05, respectively) and the IL-1β -511 C/C genotype was associated with GC (P = 0.03). The frequency of IL-1RN alleles from patients with chronic gastritis and GC indicated that there was no difference between the genotypes of the groups studied. Similar results were found for TNF-α -308 gene polymorphisms. Our results indicate that the IL-1β -511 C/C and C/T gene polymorphisms are associated with chronic gastritis and GC development in H. pylori-infected individuals.

  13. Lack of executive function deficits among adult ADHD individuals from a Brazilian clinical sample

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    Eloisa Saboya

    Full Text Available Abstract Executive function deficits have been previously documented in individuals with Attention Deficit Hyperactivity Disorder (ADHD. Objective: The current study aimed to compare measures of executive functions among a clinical sample of adults with ADHD and normal control subjects, matched for age, gender and education. Methods: Twenty-three self-referred adults diagnosed with ADHD according to DSM-IV criteria, and twenty-five control subjects were assessed using a neuropsychological battery which included the Wisconsin Card Sorting Test, Tower of Hanoi, Digit Span, Trail Making Test (A and B, Stroop Test and Raven's Progressive Matrices. Results: The ADHD group did not differ significantly from the control subjects on any of the measures assessed. Conclusion: Measures of executive functions using this test battery were unable to discriminate between adults with ADHD and control subjects in this clinical sample.

  14. Lack of executive function deficits among adult ADHD individuals from a Brazilian clinical sample

    OpenAIRE

    Eloisa Saboya; Gabriel Coutinho; Daniel Segenreich; Vanessa Ayrão; Paulo Mattos

    2009-01-01

    Abstract Executive function deficits have been previously documented in individuals with Attention Deficit Hyperactivity Disorder (ADHD). Objective: The current study aimed to compare measures of executive functions among a clinical sample of adults with ADHD and normal control subjects, matched for age, gender and education. Methods: Twenty-three self-referred adults diagnosed with ADHD according to DSM-IV criteria, and twenty-five control subjects were assessed using a neuropsychological ...

  15. Carcinoma ex pleomorphic adenoma in a Brazilian population: clinico-pathological analysis of 38 cases.

    Science.gov (United States)

    Mariano, F V; Noronha, A L F; Gondak, R O; Altemani, A M de A M; de Almeida, O P; Kowalski, L P

    2013-06-01

    Carcinoma ex pleomorphic adenoma (CXPA) is a rare tumour, with different prevalence rates reported among studies. Epidemiological studies of large series of CXPAs in developing countries are scarce. The aim of the present study was to describe Brazilian patients with CXPA; this was a retrospective study of 38 patients. Demographic and clinico-pathological features were evaluated. No preferential gender was found, and the mean age at diagnosis was 57.6 years. The most commonly involved site was the parotid, followed by the submandibular and the minor salivary glands. A prevalence of clinical stages III and IV was observed at diagnosis. The most common histological subtypes were salivary duct carcinoma, adenocarcinoma not otherwise specified, myoepithelial carcinoma, and epithelial-myoepithelial carcinoma. Moreover, by invasive phase, most were frankly invasive carcinoma. Recurrence was observed in seven out of 24 patients with outcome information available, and all were invasive cases. All seven patients died of causes related to the disease. The distributions of cases according to age, gender, tumour location, and clinical stage were similar to those reported in the literature. Frankly invasive cases presented a worse prognosis. More information is needed to further our understanding of the clinico-pathological aspects of CXPA.

  16. Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms

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    Elisa Korenblum

    2012-01-01

    Full Text Available Crude oil samples with high- and low-water content from two offshore platforms (PA and PB in Campos Basin, Brazil, were assessed for bacterial communities by 16S rRNA gene-based clone libraries. RDP Classifier was used to analyze a total of 156 clones within four libraries obtained from two platforms. The clone sequences were mainly affiliated with Gammaproteobacteria (78.2% of the total clones; however, clones associated with Betaproteobacteria (10.9%, Alphaproteobacteria (9%, and Firmicutes (1.9% were also identified. Pseudomonadaceae was the most common family affiliated with these clone sequences. The sequences were further analyzed by MOTHUR, yielding 81 operational taxonomic units (OTUs grouped at 97% stringency. Richness estimators also calculated by MOTHUR indicated that oil samples with high-water content were the most diverse. Comparison of bacterial communities present in these four samples using LIBSHUFF and Principal Component Analysis (PCA indicated that the water content significantly influenced the community structure only of crude oil obtained from PA. Differences between PA and PB libraries were observed, suggesting the importance of the oil field as a driver of community composition in this habitat.

  17. Enhancement of declarative memory associated with emotional content in a Brazilian sample

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    Frank J.E.

    2000-01-01

    Full Text Available Several studies have documented that emotional arousal may enhance long-term memory. This is an adaptation of a paradigm previously used in North American and European samples in investigations of the influence of emotion on long-term retention. A sample of 46 healthy adults of high and low educational levels watched a slide presentation of stories. A randomly assigned group watched a story with an arousing content and another group watched a neutral story. The stories were matched for structure and comprehensibility and the set and order of the 11 slides were the same in both conditions. Immediately after viewing the slide presentation, the participants were asked to rate the emotionality of the narrative. The arousing narrative was rated as being more emotional than the neutral narrative (t (44 = -3.6, P<0.001. Ten days later subjects were asked to remember the story and answer a multiple-choice questionnaire about it. The subjects who watched the arousing story had higher scores in the free recall measure (t (44 = -2.59, P<0.01. There were no differences between groups in the multiple-choice test of recognition memory (t (44 = 0.26. These findings confirm that an emotional arousing content enhances long-term declarative memory and indicate the possibility of applying this instrument to clinical samples of various cultural backgrounds.

  18. Pollen analysis in honey samples from the two main producing regions in the Brazilian northeast

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    Geni da S. Sodré

    2007-09-01

    Full Text Available Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two states of the northeastern region of Brazil, Piauí (38 samples and Ceará (20 samples, and to verify the potential of the honey plants during the months of February to August. The samples were obtained directly from beekeepers in each state and analyzed at the Apiculture Laboratory of the Entomology Section of Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, State of São Paulo, Brazil. The pollen analysis was performed using the acetolysis method. The samples were submitted to both a qualitative and a quantitative analysis. The dominant pollen in the State of Ceará is from Mimosa caesalpiniaefolia, M. verrucosa, Borreria verticillata, Serjania sp., and a Fabaceae pollen type, while in the State of Piauí it is from Piptadenia sp., M. caesalpiniaefolia, M. verrucosa, Croton urucurana and Tibouchina sp.O conhecimento da origem botânica do mel é de grande importância para o apicultor por indicar fontes adequadas e de abundante suprimento de néctar e pólen para as abelhas, contribuindo, desta forma, para uma melhor produção. O presente estudo teve como objetivo identificar os tipos polínicos em 58 amostras de méis produzidos em dois estados da região nordeste do Brasil, Piauí (38 amostras e Ceará (20 amostras, verificando o potencial das plantas apícolas durante os meses de fevereiro a agosto. As amostras foram obtidas diretamente de apicultores de cada Estado e analisadas no Laboratório de Apicultura do Setor de Entomologia da Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, Estado de São Paulo. A análise polínica foi realizada utilizando-se o método da acetólise. As amostras foram

  19. Developmental dyscalculia in children and adolescents with idiopathic epilepsies in a Brazilian sample.

    Science.gov (United States)

    Thomé, Ursula; Paixão Alves, Sandra Regina da; Guerreiro, Sabrina Mendonça; Machado da Costa, Célia Regina Carvalho; Souza Moreira, Fernanda de; Bandeira Lima, Andrea; Ferreira Tavares, Maria Rita; Souza Maia Filho, Heber

    2014-04-01

    Epilepsy is one of the most prevalent chronic disorders of childhood which can threaten child development and mental health. Among cognitive disorders, dyscalculia is one of the most important. In this study, 39 children and adolescents with idiopathic epilepsy underwent clinical and neuropsychological assessment to determine the intellectual level, math skills, reading and writing performance and neuropsychological profile. It was observed that the mathematical ability was below schooling expectations in a higher frequency than expected. There were no significant differences in mathematical performance among groups divided by number of antiepileptic drugs used, duration of disease and types and frequency of seizures. There was a positive correlation with intelligence quotient and attentional and reading level. These results suggest the existence not only of dyscalculia, but the concurrence of attentional and reading problems for the poor mathematical performance in this population.

  20. Developmental dyscalculia in children and adolescents with idiopathic epilepsies in a Brazilian sample

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    Ursula Thomé

    2014-04-01

    Full Text Available Epilepsy is one of the most prevalent chronic disorders of childhood which can threaten child development and mental health. Among cognitive disorders, dyscalculia is one of the most important. In this study, 39 children and adolescents with idiopathic epilepsy underwent clinical and neuropsychological assessment to determine the intellectual level, math skills, reading and writing performance and neuropsychological profile. It was observed that the mathematical ability was below schooling expectations in a higher frequency than expected. There were no significant differences in mathematical performance among groups divided by number of antiepileptic drugs used, duration of disease and types and frequency of seizures. There was a positive correlation with intelligence quotient and attentional and reading level. These results suggest the existence not only of dyscalculia, but the concurrence of attentional and reading problems for the poor mathematical performance in this population.

  1. 45 CFR Appendix C to Part 1356 - Calculating Sample Size for NYTD Follow-Up Populations

    Science.gov (United States)

    2010-10-01

    ... Populations C Appendix C to Part 1356 Public Welfare Regulations Relating to Public Welfare (Continued) OFFICE... Follow-Up Populations 1. Using Finite Population Correction The Finite Population Correction (FPC) is applied when the sample is drawn from a population of one to 5,000 youth, because the sample is more...

  2. Dietary exposure of Brazilian consumers to dithiocarbamate pesticides : a probabilistic approach

    NARCIS (Netherlands)

    Caldas, E.D.; Tressou, J.; Boon, P.E.

    2006-01-01

    A probabilistic estimation of the exposure of the Brazilian population to the dithiocarbamate pesticides was performed using the Monte Carlo Risk Assessment program (MCRA 3.5). Residue data, as CS(2), for 3821 samples were obtained from the Brazilian national monitoring program on pesticide residues

  3. Preliminary Analysis of the Nonsynonymous Polymorphism rs17563 in BMP4 Gene in Brazilian Population Suggests Protection for Nonsyndromic Cleft Lip and Palate

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    Tânia Kawasaki Araújo

    2012-01-01

    Full Text Available Cleft lip with or without palate (CL±P is common congenital anomalies in humans. Experimental evidence has demonstrated that bone morphogenetic protein 4 gene (Bmp4 is involved in the etiology of CL±P in animal models. The nonsynonymous polymorphism rs17563 T>C (p.V152A in the BMP4 gene has been associated to the risk of nonsyndromic CL±P in Chinese population and microforms from different ethnic backgrounds. The aim of this study was to investigate the role of BMP4 gene in CL±P in Brazilian sample using genetic association approach. Our sample was composed by 123 patients with nonsyndromic CL±P and 246 controls, in which absence of CL±P was confirmed in 3 generations. The rs17563 polymorphism was genotyped by PCR-RFLP technique. Logistic regression was performed to evaluate allele and genotype association. Our data showed statistical power to detect association (86.83% in this sample. Logistic regression results showed significant association between C allele and CL±P (P=0.00018, OR =0.40, and 95% CI = 0.25–0.65, as well as CC genotype and CL±P (P=0.00018, OR =0.35, and 95% CI = 0.19–0.66. So, there is a strong association between nonsyndromic CL±P and BMP4 rs17563 polymorphism in our sample and the C allele had a protective effect against the occurrence of nonsyndromic CL±P.

  4. Study population, questionnaire, data management and sample description

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    Chiara de Waure

    2015-06-01

    Full Text Available INTRODUCTION: This article describes methodological issues of the "Sportello Salute Giovani" project ("Youth Health Information Desk", a multicenter study aimed at assessing the health status and attitudes and behaviours of university students in Italy. MATERIALS AND METHODS: The questionnaire used to carry out the study was adapted from the Italian health behaviours in school-aged children (HBSC project and consisted of 93 items addressing: demographics; nutritional habits and status; physical activity; lifestyles; reproductive and preconception health; health and satisfaction of life; attitudes and behaviours toward academic study and new technologies. The questionnaire was administered to a pool of 12 000 students from 18 to 30 years of age who voluntary decided to participate during classes held at different Italian faculties or at the three "Sportello Salute Giovani" centers which were established in the three sites of the Università Cattolica del Sacro Cuore (Catholic University of the Sacred Heart of Rome. RESULTS: The final study sample was composed by 8516 university students. The mean age of responders was 22.2 (Standard Deviation 2.0 and 5702 (67.0% were females. According to the distribution in age classes, 3601 (43.3% belonged to the 18-21 one, 3796 (44.5% to the 22-24 class and 1019 (12.2% to the 25-30 class. With respect to socio-economic status, data were available for 8410 responders and showed that 50.3% of students belonged to the middle class. DISCUSSION: The project took into consideration a large number of individuals from different regions of the country and therefore may be considered representative of the general population of Italian university students. Furthermore, it is the first to address, at the same time, several issues, in particular attitudes and behaviours toward health, in Italian university students. CONCLUSION: The analysis of data from such a large sample of university students sets the basis for

  5. Genetic differentiation among populations of the Roseate Spoonbill (Platalea ajaja; Aves: Pelecaniformes) in three Brazilian Wetlands.

    Science.gov (United States)

    Miño, Carolina Isabel; Del Lama, Silvia Nassif

    2014-08-01

    Effective population size, levels of genetic diversity, gene flow, and genetic structuring were assessed in 205 colonial Roseate spoonbills from 11 breeding colonies from north, central west, and south Brazil. Colonies and regions exhibited similar moderate levels of diversity at five microsatellite loci (mean expected heterozygosity range 0.50-0.62; allelic richness range 3.17-3.21). The central west region had the highest Ne (59). F ST values revealed low but significant genetic structuring among colonies within the north and within the south regions. Significant global genetic structuring was found between the northern and central western populations as well as between the northern and southern populations. An individual-based Bayesian clustering method inferred three population clusters. Assignment tests correctly allocated up to 64% of individuals to their source regions. Collectively, results revealed complex demographic dynamics, with ongoing gene flow on a local scale, but genetic differentiation on a broader scale. Populations in the three regions may all be conserved, but special concern should be given to central western ones, which can significantly contribute to the species' gene pool in Brazil.

  6. Multilocus Family-Based Association Analysis of Seven Candidate Polymorphisms with Essential Hypertension in an African-Derived Semi-Isolated Brazilian Population

    Science.gov (United States)

    Kimura, L.; Angeli, C. B.; Auricchio, M. T. B. M.; Fernandes, G. R.; Pereira, A. C.; Vicente, J. P.; Pereira, T. V.; Mingroni-Netto, R. C.

    2012-01-01

    Background. It has been widely suggested that analyses considering multilocus effects would be crucial to characterize the relationship between gene variability and essential hypertension (EH). Objective. To test for the presence of multilocus effects between/among seven polymorphisms (six genes) on blood pressure-related traits in African-derived semi-isolated Brazilian populations (quilombos). Methods. Analyses were carried out using a family-based design in a sample of 652 participants (97 families). Seven variants were investigated: ACE (rs1799752), AGT (rs669), ADD2 (rs3755351), NOS3 (rs1799983), GNB3 (rs5441 and rs5443), and GRK4 (rs1801058). Sensitivity analyses were further performed under a case-control design with unrelated participants only. Results. None of the investigated variants were associated individually with both systolic and diastolic BP levels (SBP and DBP, respectively) or EH (as a binary outcome). Multifactor dimensionality reduction-based techniques revealed a marginal association of the combined effect of both GNB3 variants on DBP levels in a family-based design (P = 0.040), whereas a putative NOS3-GRK4 interaction also in relation to DBP levels was observed in the case-control design only (P = 0.004). Conclusion. Our results provide limited support for the hypothesis of multilocus effects between/among the studied variants on blood pressure in quilombos. Further larger studies are needed to validate our findings. PMID:23056922

  7. Mitotic and meiotic chromosomes of a southern Brazilian population of Boophilus microplus (Acari, Ixodidae

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    Rosane Nunes Garcia

    Full Text Available Using conventional staining with acetic orcein and C-banding techniques it was investigated constitutive heterochromatin chromosomal polymorphisms and the mitotic and the meiotic behavior of male and female chromosomes of Boophilus microplus (Canestrini, 1887. Some differences were detected in the population of southern Brazil as compared to the data of other authors for populations in other latitudes. The differences being mainly concerned with the distribution of constitutive centromeric heterochromatin and variation in the length of heterochromatic blocks in the pericentromeric regions of some chromosome pairs.

  8. Population Genetics of Jaguars (Panthera onca) in the Brazilian Pantanal: Molecular Evidence for Demographic Connectivity on a Regional Scale.

    Science.gov (United States)

    Valdez, Fernanda Pedone; Haag, Taiana; Azevedo, Fernando C C; Silveira, Leandro; Cavalcanti, Sandra M C; Salzano, Francisco M; Eizirik, Eduardo

    2015-01-01

    Habitat loss and fragmentation are important threats to carnivores worldwide, and can be especially intense for large predators. Jaguars have already been extirpated from over half of their original area of distribution, and few regions still maintain large populations. For these, detailed understanding is crucial for setting appropriate recovery targets in impacted areas. The Pantanal is among the best examples of a region with a large jaguar population in a healthy environment. Here, we analyzed 12 microsatellite loci to characterize genetic diversity and population structure of 52 jaguars sampled in 4 localities of the southern Pantanal, and compared them with prior studies of heavily fragmented populations of the Atlantic Forest. Although we observed some internal structure among the Pantanal localities, our results indicated that this area comprises a single population with high genetic variability. Moreover, our comparative analyses supported the hypothesis that the strong population structure observed in the Atlantic Forest derives from recent, anthropogenic fragmentation. We also observed significant but low levels of genetic differentiation between the Pantanal and Atlantic Forest populations, indicating recent connectivity between jaguars occurring in these biomes. Evidence for admixture between the Pantanal and a population on the western boundary of the Atlantic Forest corroborates the transitional nature of the latter area, where the jaguar population has already been extirpated. Our results can be used to understand jaguar population dynamics in a region that is less disturbed than the Atlantic forest, and to support the design of conservation strategies that maintain and restore natural connectivity among currently isolated areas.

  9. High frequency of the CCR5delta32 variant among individuals from an admixed Brazilian population with sickle cell anemia

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    J.A.B. Chies

    2003-01-01

    Full Text Available Homozygous sickle cell disease (SCD has a wide spectrum of clinical manifestations. In Brazil, the main cause of death of individuals with SCD is recurrent infection. The CCR5delta32 allele, which confers relative resistance to macrophage-tropic HIV virus infection, probably has reached its frequency and world distribution due to other pathogens that target macrophage in European populations. In the present investigation a relatively higher prevalence (5.1% of the CCR5delta32 allele was identified, by PCR amplification using specific primers, in 79 SCD patients when compared to healthy controls (1.3% with the same ethnic background (Afro-Brazilians. Based on a hypothesis that considers SCD as a chronic inflammatory condition, and since the CCR5 chemokine receptor is involved in directing a Th1-type immune response, we suggest that a Th1/Th2 balance can influence the morbidity of SCD. If the presence of the null CCR5delta32 allele results in a reduction of the chronic inflammation state present in SCD patients, this could lead to differential survival of SCD individuals who are carriers of the CCR5delta32 allele. This differential survival could be due to the development of less severe infections and consequently reduced or less severe vaso-occlusive crises.

  10. Seasonal Variation in Population Abundance and Chytrid Infection in Stream-Dwelling Frogs of the Brazilian Atlantic Forest.

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    Joice Ruggeri

    Full Text Available Enigmatic amphibian declines were first reported in southern and southeastern Brazil in the late 1980s and included several species of stream-dwelling anurans (families Hylodidae and Cycloramphidae. At that time, we were unaware of the amphibian-killing fungus Batrachochytrium dendrobatidis (Bd; therefore, pollution, habitat loss, fragmentation and unusual climatic events were hypothesized as primary causes of these declines. We now know that multiple lineages of Bd have infected amphibians of the Brazilian Atlantic forest for over a century, yet declines have not been associated specifically with Bd outbreaks. Because stream-dwelling anurans occupy an environmental hotspot ideal for disease transmission, we investigated temporal variation in population and infection dynamics of three stream-adapted species (Hylodes asper, H. phyllodes, and Cycloramphus boraceiensis on the northern coast of São Paulo state, Brazil. We surveyed standardized transects along streams for four years, and show that fluctuations in the number of frogs correlate with specific climatic variables that also increase the likelihood of Bd infections. In addition, we found that Bd infection probability in C. boraceiensis, a nocturnal species, was significantly higher than in Hylodes spp., which are diurnal, suggesting that the nocturnal activity may either facilitate Bd zoospore transmission or increase susceptibility of hosts. Our findings indicate that, despite long-term persistence of Bd in Brazil, some hosts persist with seasonally variable infections, and thus future persistence in the face of climate change will depend on the relative effect of those changes on frog recruitment and pathogen proliferation.

  11. Personality disorder and substance related disorders: a six-month follow-up study with a Brazilian sample

    Directory of Open Access Journals (Sweden)

    Débora M. Krieger

    2016-06-01

    Full Text Available ABSTRACT Objectives A few Brazilian researches correlate personality disorders (PD and substance related disorders (SRD. The aim of the present study is to investigate the association between them, to evaluate the PD frequency among chemical dependents inpatients, this comorbidity association with social and demographic characteristics, used drug of choice, its impact on clinical evolution until the moment of their committal, the frequency of relapse, self-help group – and psychotherapic adherence among SRD patients six months following committal. Methods A 101 inpatients sample of chemical dependents was enrolled in 2 hospitals. The following instruments were applied: a questionnaire for social and demographic characteristics identification and drug use pattern, some questions from the sixth version of the Addiction Severity Index (ASI-6, the SCID-II questionnaire and specific questions concerning psychotherapic and self-help groups participation, and medication use. Results From these 101 patients, 55.4% were diagnosed with PD, being avoidant (14.9%, borderline (11.9% and antisocial (8.9% the more frequent ones found. PD patients had an earlier crack use in life (p = 0.038 and had also more previous treatments than the ones without PD (p = 0.005. Borderline PD patients were less worried to substance use problem (p = 0.003. After 6-months follow-up, no statistical significance was found between patients with and without PD regarding drug use or treatment adherence. Conclusion A high PD diagnosis was found in drug use inpatients. Patients diagnosed with SRD and PD need the identification of this comorbidity and of their personality characteristics in order to plan a more comprehensive and effective treatment.

  12. Population Genetics of GYPB and Association Study between GYPB*S/s Polymorphism and Susceptibility to P. falciparum Infection in the Brazilian Amazon

    Science.gov (United States)

    Amaral, Daphne R. T.; Costa, Daiane C.; Furlani, Natália G.; Zuccherato, Luciana W.; Machado, Moara; Reid, Marion E.; Zalis, Mariano G.; Rossit, Andréa R.; Santos, Sidney E. B.; Machado, Ricardo L.; Lustigman, Sara

    2011-01-01

    Background Merozoites of Plasmodium falciparum invade through several pathways using different RBC receptors. Field isolates appear to use a greater variability of these receptors than laboratory isolates. Brazilian field isolates were shown to mostly utilize glycophorin A-independent invasion pathways via glycophorin B (GPB) and/or other receptors. The Brazilian population exhibits extensive polymorphism in blood group antigens, however, no studies have been done to relate the prevalence of the antigens that function as receptors for P. falciparum and the ability of the parasite to invade. Our study aimed to establish whether variation in the GYPB*S/s alleles influences susceptibility to infection with P. falciparum in the admixed population of Brazil. Methods Two groups of Brazilian Amazonians from Porto Velho were studied: P. falciparum infected individuals (cases); and uninfected individuals who were born and/or have lived in the same endemic region for over ten years, were exposed to infection but have not had malaria over the study period (controls). The GPB Ss phenotype and GYPB*S/s alleles were determined by standard methods. Sixty two Ancestry Informative Markers were genotyped on each individual to estimate admixture and control its potential effect on the association between frequency of GYPB*S and malaria infection. Results GYPB*S is associated with host susceptibility to infection with P. falciparum; GYPB*S/GYPB*S and GYPB*S/GYPB*s were significantly more prevalent in the in the P. falciparum infected individuals than in the controls (69.87% vs. 49.75%; P<0.02). Moreover, population genetics tests applied on the GYPB exon sequencing data suggest that natural selection shaped the observed pattern of nucleotide diversity. Conclusion Epidemiological and evolutionary approaches suggest an important role for the GPB receptor in RBC invasion by P. falciparum in Brazilian Amazons. Moreover, an increased susceptibility to infection by this parasite is

  13. Population genetics of GYPB and association study between GYPB*S/s polymorphism and susceptibility to P. falciparum infection in the Brazilian Amazon.

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    Eduardo Tarazona-Santos

    Full Text Available BACKGROUND: Merozoites of Plasmodium falciparum invade through several pathways using different RBC receptors. Field isolates appear to use a greater variability of these receptors than laboratory isolates. Brazilian field isolates were shown to mostly utilize glycophorin A-independent invasion pathways via glycophorin B (GPB and/or other receptors. The Brazilian population exhibits extensive polymorphism in blood group antigens, however, no studies have been done to relate the prevalence of the antigens that function as receptors for P. falciparum and the ability of the parasite to invade. Our study aimed to establish whether variation in the GYPB*S/s alleles influences susceptibility to infection with P. falciparum in the admixed population of Brazil. METHODS: Two groups of Brazilian Amazonians from Porto Velho were studied: P. falciparum infected individuals (cases; and uninfected individuals who were born and/or have lived in the same endemic region for over ten years, were exposed to infection but have not had malaria over the study period (controls. The GPB Ss phenotype and GYPB*S/s alleles were determined by standard methods. Sixty two Ancestry Informative Markers were genotyped on each individual to estimate admixture and control its potential effect on the association between frequency of GYPB*S and malaria infection. RESULTS: GYPB*S is associated with host susceptibility to infection with P. falciparum; GYPB*S/GYPB*S and GYPB*S/GYPB*s were significantly more prevalent in the in the P. falciparum infected individuals than in the controls (69.87% vs. 49.75%; P<0.02. Moreover, population genetics tests applied on the GYPB exon sequencing data suggest that natural selection shaped the observed pattern of nucleotide diversity. CONCLUSION: Epidemiological and evolutionary approaches suggest an important role for the GPB receptor in RBC invasion by P. falciparum in Brazilian Amazons. Moreover, an increased susceptibility to infection by this

  14. Chemical variation of tannins and triterpenes in Brazilian populations of Maytenus ilicifolia Mart. Ex Reiss

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    AJ. Mossi

    Full Text Available Maytenus ilicifolia and Maytenus aquifolia species are widely used as a cicatrisation agent, an anti-spasmodic, contraceptive, antiulcerogenic, diuretic and analgesic. Considering the importance of these species in popular medicine, this work is focused on the determination of the chemical content of tannins and the triterpenes friedelan-3-one, friedelan-3-ol and friedelin in 15 native populations of Maytenus ilicifolia distributed in the south and mind-west regions of Brazil. Correlation of the concentration of these compounds with the environmental parameters such as average annual temperature, climate, vegetation, geomorphology, latitude and altitude was determined using Pearson's coefficient. Results showed that average annual temperature and climate have significant effect on tannin content at a 95% confidence level. The highest tannin concentration was found in Ponta Porã population, and for the triterpenes investigated, a significant correlation between their concentrations with the environmental variables studied was not verified.

  15. Cluster evaluation of Brazilian and Moroccan goat populations using physical measurements

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    Luanna Chácara Pires

    2013-10-01

    Full Text Available The aim of this study was to compare the genetic diversity of 12 populations of goats in Brazil and Morocco (n = 796 through the use of physical measurements and different multivariate techniques. Traits measured included wither height (WH, distance from the brisket to the ground (BH and ear length (EL. The standardized Euclidean distance (D was adopted. The D values were submitted to clustering analysis using hierarchical methods (from nearest neighbor and UPGMA - Unweighted Pair Group Method with Arithmetic Mean and the numbers of clusters were analyzed using the Tocher optimization method. The population clustering was different depending on the method of analysis used. Among the hierarchical methods, UPGMA showed the best fit (CCC = 0.82. The Tocher method enabled the formation of four different clusters. Although the hierarchical and Tocher methods resulted in different cluster formations, both contributed to the interpretation of the genetic cluster divergence. The results obtained through UPGMA and Tocher optimization enable their use for future studies that may include a larger number of biometric variables on greater numbers of individuals and additional populations.

  16. CYP2A6 and CYP2E1 polymorphisms in a Brazilian population living in Rio de Janeiro

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    A. Rossini

    2006-02-01

    Full Text Available Cytochrome P450 (CYP is a superfamily of enzymes involved in the metabolism of endogenous compounds and xenobiotics. CYP2A6 catalyzes the oxidation of nicotine and the activation of carcinogens such as aflatoxin B1 and nitrosamines. CYP2E1 metabolizes ethanol and other low-molecular weight compounds and can also activate nitrosamines. The CYP2A6 and CYP2E1 genes are polymorphic, altering their catalytic activities and susceptibility to cancer and other diseases. A number of polymorphisms described are ethnic-dependent. In the present study, we determined the genotype and allele frequencies of the main CYP2A6 and CYP2E1 polymorphisms in a group of 289 volunteers recruited at the Central Laboratory of Hospital Universitário Pedro Ernesto. They had been residing in the city of Rio de Janeiro for at least 6 months and were divided into two groups according to skin color (white and non-white. The alleles were determined by allele specific PCR (CYP2A6 or by PCR-RFLP (CYP2E1. The frequencies of the CYP2A6*1B and CYP2A6*2 alleles were 0.29 and 0.02 for white individuals and 0.24 and 0.01 for non-white individuals, respectively. The CYP2A6*5 allele was not found in the population studied. Regarding the CYP2E1*5B allele, we found a frequency of 0.07 in white individuals, which was statistically different (P < 0.05 from that present in non-white individuals (0.03. CYP2E1*6 allele frequency was the same (0.08 in both groups. The frequencies of CYP2A6*1B, CYP2A6*2 and CYP2E1*6 alleles in Brazilians are similar to those found in Caucasians and African-Americans, but the frequency of the CYP2E1*5B allele is higher in Brazilians.

  17. CYP2A6 and CYP2E1 polymorphisms in a Brazilian population living in Rio de Janeiro

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    Rossini A.

    2006-01-01

    Full Text Available Cytochrome P450 (CYP is a superfamily of enzymes involved in the metabolism of endogenous compounds and xenobiotics. CYP2A6 catalyzes the oxidation of nicotine and the activation of carcinogens such as aflatoxin B1 and nitrosamines. CYP2E1 metabolizes ethanol and other low-molecular weight compounds and can also activate nitrosamines. The CYP2A6 and CYP2E1 genes are polymorphic, altering their catalytic activities and susceptibility to cancer and other diseases. A number of polymorphisms described are ethnic-dependent. In the present study, we determined the genotype and allele frequencies of the main CYP2A6 and CYP2E1 polymorphisms in a group of 289 volunteers recruited at the Central Laboratory of Hospital Universitário Pedro Ernesto. They had been residing in the city of Rio de Janeiro for at least 6 months and were divided into two groups according to skin color (white and non-white. The alleles were determined by allele specific PCR (CYP2A6 or by PCR-RFLP (CYP2E1. The frequencies of the CYP2A6*1B and CYP2A6*2 alleles were 0.29 and 0.02 for white individuals and 0.24 and 0.01 for non-white individuals, respectively. The CYP2A6*5 allele was not found in the population studied. Regarding the CYP2E1*5B allele, we found a frequency of 0.07 in white individuals, which was statistically different (P < 0.05 from that present in non-white individuals (0.03. CYP2E1*6 allele frequency was the same (0.08 in both groups. The frequencies of CYP2A6*1B, CYP2A6*2 and CYP2E1*6 alleles in Brazilians are similar to those found in Caucasians and African-Americans, but the frequency of the CYP2E1*5B allele is higher in Brazilians.

  18. Epidemiology of multimorbidity within the Brazilian adult general population: Evidence from the 2013 National Health Survey (PNS 2013)

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    de Azevedo-Marques, João Mazzoncini; Coxon, Domenica; Santos, Jair Lício Ferreira

    2017-01-01

    Middle-income countries are facing a growing challenge of adequate health care provision for people with multimorbidity. The objectives of this study were to explore the distribution of multimorbidity and to identify patterns of multimorbidity in the Brazilian general adult population. Data from 60202 adults, aged ≥18 years that completed the individual questionnaire of the National Health Survey 2013 (Portuguese: “Pesquisa Nacional de Saúde”–“PNS”) was used. We defined multimorbidity as the presence of two or more chronic conditions, including self-reported diagnoses and responses to the 9-item Patient Health Questionnaire for depression. Multivariate Poisson regression analyses were used to explore relationship between multimorbidity and demographic factors. Exploratory tetrachoric factor analysis was performed to identify multimorbidity patterns. 24.2% (95% CI 23.5–24.9) of the study population were multimorbid, with prevalence rate ratios being significantly higher in women, older people and those with lowest educational level. Multimorbidity occurred earlier in women than in men, with half of the women and men aged 55–59 years and 65–69 years, respectively, were multimorbid. The absolute number of people with multimorbidity was approximately 2.5-fold higher in people younger than 65 years than older counterparts (9920 vs 3945). Prevalence rate ratios of any mental health disorder significantly increased with the number of physical conditions. 46.7% of the persons were assigned to at least one of three identified patterns of multimorbidity, including: “cardio-metabolic”, “musculoskeletal-mental” and “respiratory” disorders. Multimorbidity in Brazil is as common as in more affluent countries. Women in Brazil develop diseases at younger ages than men. Our findings can inform a national action plan to prevent multimorbidity, reduce its burden and align health-care services more closely with patients’ needs. PMID:28182778

  19. Population biology of the crab Armases angustipes (Crustacea, Decapoda, Sesarmidae at Brazilian tropical coast

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    Marina de Sá Leitão C. de Araújo

    2014-06-01

    Full Text Available The semi terrestrial crabs are important elements of the fauna of coastal regions. The aim of this study was to analyze the population structure of Armases angustipes (Dana, 1852 at estuaries of the Ariquindá River, considered a non impacted area, and Mamucabas River, considered a few impacted area, on the south coast of state of Pernambuco, Brazil. The species occurred in all months of the year. The number of individuals per month varied, being higher in the months of transition between the seasons. This is probably due to significant seasonal variations of air and burrow temperature and burrow salinity. There was no sexual dimorphism in size of A. angustipes in the mangrove of Ariquindá River, but males were larger than females in the mangrove of Mamucabas River. In both estuaries, the sex ratio did not differ from Mendelian proportion, but showed a deviation for females. The analysis of temporal variation in sex ratio showed significant differences in some months of the year. These variations are due to cyclical events that act distinctly on each sex. In both estuaries, size classes of carapace width were equally represented by both sexes. The ovigerous females of A. angustipes occurred only in some months of the year, especially in summer, in both estuaries. Probably the high phytoplankton productivity observed in summer favors the reproductive activity, since these algae serve as food for the larvae. Specimens of the population of Rio Ariquindá are largest and wider than those of Mamucabas River. This fact, associated with the low abundance of crabs and the lower frequency of ovigerous females observed in Mamucabas River, is an indication that this population may be influenced by the environmental impacts that this estuary has received.

  20. A Data Mining Approach to Identify Sexuality Patterns in a Brazilian University Population.

    Science.gov (United States)

    Waleska Simões, Priscyla; Cesconetto, Samuel; Toniazzo de Abreu, Larissa Letieli; Côrtes de Mattos Garcia, Merisandra; Cassettari Junior, José Márcio; Comunello, Eros; Bisognin Ceretta, Luciane; Aparecida Manenti, Sandra

    2015-01-01

    This paper presents the profile and experience of sexuality generated from a data mining classification task. We used a database about sexuality and gender violence performed on a university population in southern Brazil. The data mining task identified two relationships between the variables, which enabled the distinction of subgroups that better detail the profile and experience of sexuality. The identification of the relationships between the variables define behavioral models and factors of risk that will help define the algorithms being implemented in the data mining classification task.

  1. Neuropsychological profiles correlated with clinical and behavioral impairments in a sample of Brazilian children with Attention Deficit Hyperactivity Disorder (ADHD

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    Sueli eRizzutti

    2015-11-01

    Full Text Available ADHD is a complex neurodevelopmental disorder that implies several-step process and there is no single test to diagnose both ADHD and associated comorbidities such as oppositional defiant disorder, anxiety disorder, depression and certain types of learning disabilities. The purpose of the present study was to examine correlations between behavioral and clinical symptoms by administering an extensive neuropsychological battery to a sample of children and adolescents from a developing country. The sample was divided into three groups: non-ADHD; ADHD-non-comorbid; and ADHD+comorbidity. A full neuropsychological battery and clinical assessment found that 105 children met DSM-5 criteria, of whom 46.6% had the predominantly inattentive presentation, 37.3% had combined presentation and 16% were predominantly hyperactive/impulsive presentation. The internal correlation between neuropsychological tests did not reach statistical significance in the comparison between ADHD and non-ADHD cases (p<0.17. Clinical ADHD cases, including both +comorbidity and non-comorbid groups, performed substantially worse on CPT, working memory. Comparing ADHD-non-comorbid and ADHD+comorbidity groups, the latter did significantly worse on inhibitory control, time processing and the level of perseveration response on CPT indexes, as well as on working memory performance and CBCL tests particularly the CBCL-DESR (deficient emotional self-regulation test in the ADHD+comorbidity group. Children diagnosed as oppositional-defiant (ODD or with conduct disorder (CD showed close correlations between clinical CBCL profiles and externalized symptoms. Our findings suggest that ADHD+comorbidity and ADHD non-comorbid cases may be differentiated by a number of neuropsychological measures, such as processing speed, inhibitory control and working memory, that may reflect different levels of involvement of the hot and cool executive domains, which are more impaired in cases of severe

  2. How well is hypertension managed in the community? A population-based survey in a Brazilian city

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    Roberto X. Piccini

    Full Text Available There is usually little information available on how well hypertensive individuals are managed at the community level. This survey measured the frequency of hypertension in a medium-sized Brazilian city by studying a cluster sample of 1657 adults aged 20-69 years. The 328 hypertensives (19.8% answered a questionnaire on the knowledge and management of their condition. Two-thirds were aware of their status and more than half were on antihypertensive medication, but only one-third had their blood pressure under control. Physicians failed to advise a large proportion of their patients about the need to lose weight, take exercise, and quit smoking. Although laboratory tests were carried out in most patients, fundoscopy and chest X-rays were performed in fewer than 50%. Continued care by the same physician was the only factor significantly associated with compensated high blood pressure (relative risk for not having continued care = 1.35; 95% CI = 1.02-1.71. High levels of absenteeism, reduction of the work load, and early retirement were found among the individuals with high blood pressure.

  3. How well is hypertension managed in the community? A population-based survey in a Brazilian city

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    Piccini Roberto X.

    1997-01-01

    Full Text Available There is usually little information available on how well hypertensive individuals are managed at the community level. This survey measured the frequency of hypertension in a medium-sized Brazilian city by studying a cluster sample of 1657 adults aged 20-69 years. The 328 hypertensives (19.8% answered a questionnaire on the knowledge and management of their condition. Two-thirds were aware of their status and more than half were on antihypertensive medication, but only one-third had their blood pressure under control. Physicians failed to advise a large proportion of their patients about the need to lose weight, take exercise, and quit smoking. Although laboratory tests were carried out in most patients, fundoscopy and chest X-rays were performed in fewer than 50%. Continued care by the same physician was the only factor significantly associated with compensated high blood pressure (relative risk for not having continued care = 1.35; 95% CI = 1.02-1.71. High levels of absenteeism, reduction of the work load, and early retirement were found among the individuals with high blood pressure.

  4. Resistance Status to the Insecticides Temephos, Deltamethrin, and Diflubenzuron in Brazilian Aedes aegypti Populations

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    Bellinato, Diogo Fernandes; Viana-Medeiros, Priscila Fernandes; Araújo, Simone Costa; Martins, Ademir J; Lima, José Bento Pereira; Valle, Denise

    2016-01-01

    Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethrin (PY), and diflubenzuron (IGR) of A. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. High levels of resistance to ne...

  5. Male-specific contributions to the Brazilian population of Espirito Santo.

    Science.gov (United States)

    de F Figueiredo, Raquel; Ambrosio, Isabela B; Braganholi, Danilo F; Chemale, Gustavo; Martins, Joyce A; Gomes, Veronica; Gusmão, Leonor; Cicarelli, Regina M B

    2016-05-01

    Y chromosome markers have been widely studied due to their various applications in the fields of forensic and evolutionary genetics. In this study, 35 Y-SNPs and 17 Y-STRs were genotyped in 253 males from the State of Espirito Santo, Brazil. A total of 18 haplogroups and 243 haplotypes were detected; the haplogroup and haplotype diversities were 0.7794 and 0.9997, respectively. Genetic distance analysis using the Y-STR data showed no statistically significant differences between Espirito Santo and other admixed populations from Brazil. The classification of paternal lineages based on haplogroups showed a predominant European contribution (85.88%), followed by African (11.37%) and Amerindian (2.75%) contributions.

  6. B-chromosomes in two Brazilian populations of Dendropsophus nanus (Anura, Hylidae

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    Lilian R. Medeiros

    2006-01-01

    Full Text Available We report on the presence of B-chromosomes in two populations of Dendropsophus nanus (= Hyla nana Boulenger, 1889 from São Paulo State, Brazil. Such chromosomes were observed in 4 out of 43 specimens (9.3% and in 9 out of 15 specimens (60% from the municipalities of Nova Aliança and Botucatu, respectively. The karyotype 2n = 30 + 1B found in D. nanus was similar to that of other species with 2n = 30 chromosomes, except for the presence of an additional small telocentric chromosome. In one specimen from Botucatu, cells with one to three extra chromosomes were observed. These B-chromosomes appeared as univalent in meiosis I and did not bear a nucleolar organizer region or exhibit constitutive heterochromatin.

  7. Ethnicity and glutathione S-transferase (GSTM1/GSTT1 polymorphisms in a Brazilian population

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    G.J.F. Gattás

    2004-04-01

    Full Text Available The distribution of polymorphisms related to glutathione S-transferases (GST has been described in different populations, mainly for white individuals. We evaluated the distribution of GST mu (GSTM1 and theta (GSTT1 genotypes in 594 individuals, by multiplex PCR-based methods, using amplification of the exon 7 of CYP1A1 gene as an internal control. In São Paulo, 233 whites, 87 mulattos, and 137 blacks, all healthy blood-donor volunteers, were tested. In Bahia, where black and mulatto populations are more numerous, 137 subjects were evaluated. The frequency of the GSTM1 null genotype was significantly higher among whites (55.4% than among mulattos (41.4%; P = 0.03 and blacks (32.8%; P < 0.0001 from São Paulo, or Bahian subjects in general (35.7%; P = 0.0003. There was no statistically different distribution among any non-white groups. The distribution of GSTT1 null genotype among groups did not differ significantly. The agreement between self-reported and interviewer classification of skin color in the Bahian group was low. The interviewer classification indicated a gradient of distribution of the GSTM1 null genotype from whites (55.6% to light mulattos (40.4%, dark mulattos (32.0% and blacks (28.6%. However, any information about race or ethnicity should be considered with caution regarding the bias introduced by different data collection techniques, specially in countries where racial admixture is intense, and ethnic definition boundaries are loose. Because homozygous deletions of GST gene might be associated with cancer risk, a better understanding of chemical metabolizing gene distribution can contribute to risk assessment of humans exposed to environmental carcinogens.

  8. Profile of a Brazilian population with severe chronic obstructive pulmonary disease

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    Yaksic Mateo Sainz

    2003-01-01

    Full Text Available Chronic obstructive pulmonary disease (COPD is a public health problem. Tobacco smoking is the major cause, but not the only one. Air pollution, exposure to chemicals, environmental smoke exposure, and passive smoking are among other contributing causes; being viral and bacterial infections also risk factors. Gender and weight are associated to the severity of the disease. Co-morbidity is frequent. OBJECTIVE: To characterize a population of COPD outpatients followed at an outsourced medical service. METHODS: Questionnaires were applied to patients with COPD. The data included gender, age, weight, body mass index (BMI, oxygen delivery users, and FEV1, exposure to tobacco smoke, exposure to wood smoke, history of tuberculosis and co-morbid diseases. RESULTS: Of the 70 patients enrolled in the study, 70% (49 were men with an average age of 64 ± 10 years, average weight of 63 ± 16 kg and average BMI of 22 ± 5 kg/m². Mean FEV1 was 35 ± 14% and 45.7% were oxygen dependent. Nine (12.8% patients never smoked, while 78.8% had quit tobacco smoking, (38 ± 11 pack/years was the average. Nine (12.8% smoked corn husk cigarettes. Eighteen (25.7% were exposed to wood smoke. Eleven (15.7% patients had tuberculosis, 5.7% complained of asthma symptoms, 2.8% had bronchiectasis, 11.4% diabetes mellitus, 51.4% hypertension, and 20% Cor pulmonale. CONCLUSION: Other possible COPD etiologies must be investigated. Determinants of the pulmonary injury could be environmental smoke exposure associated to former infections. Men with low BMI are typically representative of this severe patient population. Hypertension and Cor Pulmonale are frequent co-morbidity factors.

  9. Validity of periodontitis screening questions in a Brazilian adult population-based study

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    Renato Quirino RAMOS

    Full Text Available Abstract Population-based studies assessing self-reported periodontal questions in low-income countries are lacking, and therefore we aimed to assess the accuracy of self-reported periodontal items in Brazil. One thousand one hundred and forty adults from Florianópolis, Brazil, had their periodontium clinically examined, and responded to the following self-reported items on periodontal conditions: Question (Q1, Do you have any wobbly teeth?; Q2, Do your gums usually bleed?; and Q3, Has your dentist ever told you that you have gum disease? Periodontitis was defined as: a. ≥ 6.0 mm periodontal pocket and ≥ 4.0 mm clinical attachment loss in the same tooth, in at least one tooth (PD1; or b. ≥ 6.0 mm periodontal pocket and ≥ 4.0 mm clinical attachment loss, not necessarily in the same tooth (PD2. Sensitivity (SN and specificity (SP were calculated, and analyses were stratified by socioeconomic status and time since last dental visit. Scores were generated in order to determine the accuracy of the whole set of items. Receiver operating characteristic (ROC curves were plotted. Prevalence of clinically diagnosed periodontitis was 2.6% (95%CI = 1.7–4.0% for PD1 and 3.8% (95%CI = 2.7–5.3% for PD2. Prevalence of self-reported periodontitis varied between 2.7 (Q2 and 22.0% (Q3. SN and SP ranged between 0.0–60.0% and 73.3–98.6%, respectively; Q1 showed the highest accuracy (140.8% followed by Q3 (140.0%. The combined score of the three self-reported items did not improve accuracy estimates; the areas under the ROC curves were 0.70 and 0.68 for PD1 and PD2, respectively. The accuracy of self-reported items was low, and further studies are needed in order to develop valid and reliable periodontitis screening questions for population-based studies.

  10. Genetic structure of red-handed howler monkey populations in the fragmented landscape of Eastern Brazilian Amazonia

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    Heitor B. Bastos

    2010-01-01

    Full Text Available We genotyped 15 microsatellite loci in order to evaluate the effects of habitat fragmentation, caused by flooding of the Tucuruí reservoir, on the genetic structure of Alouatta belzebul in eastern Amazonia. The analysis included two populations sampled in 1984, representing both margins of the Tocantins river, and three populations sampled 18 years later. Minimal differences in the diversity levels between present-day (Ho = 0.62-0.69 and A R = 6.07-7.21 and pre-flooding (Ho = 0.60-0.62 and A R = 6.27-6.77 populations indicated there was no significant loss of genetic variability, possibly because of successful management strategies applied during the flooding. The changes observed were limited to shifts in the composition of alleles, which presumably reflect the admixture of subpopulations during flooding. Given this, there were significant differences in the Rst values (p = 0.05 in all but one between-site comparison. Both present-day and original populations showed a deficit of heterozygotes, which suggests that this may be typical of the species, at least at a local level, perhaps because of specific ecological characteristics. The relatively large number of private alleles recorded in all populations may be a consequence of the Wahlund effect resulting from population admixture or a process of expansion rather than the loss of rare alleles through genetic drift. Additionally, the levels of genetic variability observed in this study were higher than those reported for other species of Neotropical primates, suggesting good fitness levels in these A. belzebul populations. Regular genetic monitoring of remnant populations, especially on islands, should nevertheless be an integral component of long-term management strategies.

  11. Coffee has hepatoprotective benefits in Brazilian patients with chronic hepatitis C even in lower daily consumption than in American and European populations.

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    Machado, Silmara Rodrigues; Parise, Edison Roberto; Carvalho, Luciana de

    2014-01-01

    The potential role of coffee as a hepatoprotective substance for chronic liver diseases has been widely discussed. Our main aim was to evaluate the effect of coffee intake regarding clinical, biochemical tests and liver biopsy data in treatment naïve patients with chronic hepatitis C. One hundred and thirty-six patients with chronic hepatitis C, diagnosed through liver biopsy, or by means of clinical, ultrasound or endoscopic signs of cirrhosis, were assessed by determination of biochemical tests, metabolic and morphological alterations. Food frequency was scrutinized by using a structured questionnaire. Coffee intake represented more than 90% of the total daily caffeine, and the 75th percentile was 4-Brazilian coffee-cup/day (≥ 255 mL/day or ≥ 123 mg caffeine/day). According to caffeine intake, patients were divided into two groups (coffee intake has hepatoprotective benefits for Brazilian patients with chronic hepatitis C, even in lower doses than that of American and European population intake.

  12. Eating practices and habitus in mothers. A Brazilian population-based survey.

    Science.gov (United States)

    de Morais Sato, Priscila; da Rocha Pereira, Patrícia; de Carvalho Stelmo, Isis; Unsain, Ramiro Fernandez; Ulian, Mariana Dimitrov; Sabatini, Fernanda; Martins, Paula Andrea; Scagliusi, Fernanda Baeza

    2014-11-01

    A population-based cross-sectional study was conducted with mothers living in the city of Santos, Brazil, in order to investigate their eating practices, and the interface between those practices and the concept of habitus. From a cluster analysis of the scores for dietary pattern and for food preparation and consumption, the mothers were categorised into five clusters of eating practices: practical mothers (19.8%), symbiotic mothers (3.2%), health-conscious hedonists (17.3%), traditionalists (34.6%), and family cooks (25.1%). To access the habitus of the eating-practice clusters, the following variables were compared: location of residence, profession, socioeconomic status, weight-loss practices, risk behaviours for eating disorders, disordered eating attitudes, body dissatisfaction, and cultural and technological consumption. For all the groups, the observed eating practices were permeated by responsibility for the family's diet, but with different manifestations. For symbiotic mothers, practical mothers, and family cooks, the primary function of their relation with food was to nourish their families, with little expression of their own tastes and preferences. The traditionalists and the health-conscious hedonists, on the other hand, manifested their role as mothers by providing food considered 'nutritionally proper' to their family members. Furthermore, aspects of contemporary lifestyles, such as little time for food, individualisation of meals, and consumption of processed foods, were found to coexist with the valorisation and maintenance of the traditional meals within some groups. The variety of eating practices could not be understood as a linear association between economic and cultural capitals; however, eating practices seemed to interact with those capitals, composing a habitus.

  13. CODIS STR loci data from 41 sample populations.

    Science.gov (United States)

    Budowle, B; Shea, B; Niezgoda, S; Chakraborty, R

    2001-05-01

    Allele distributions for 12 or 13 CODIS core tetrameric short tandem repeat (STR) loci CSFIPO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA were determined in 41 population data sets. The major population groups comprise African Americans, U.S. Caucasians, Hispanics, Far East Asians, and Native Americans. There was little evidence for departures from Hardy-Weinberg expectations (HWE) in any of the populations. The FST estimates over all thirteen STR loci are 0.0006 for African Americans, -0.0005 for Caucasians, 0.0021 for Hispanics, 0.0039 for Asians, and 0.0282 for Native Americans.

  14. Association between tooth loss and obesity in Brazilian adults: a population-based study

    Directory of Open Access Journals (Sweden)

    Carla de Oliveira Bernardo

    2012-10-01

    Full Text Available OBJECTIVE: To examine the association between tooth loss and general and central obesity among adults. METHODS: Population-based cross-sectional study with 1,720 adults aged 20 to 59 years from Florianópolis, Southern Brazil. Home interviews were performed and anthropometric measures were taken. Information on sociodemographic data, self-reported diabetes, self-reported number of teeth, central obesity (waist circumference [WC] > 88 cm in women and > 102 cm in men and general obesity (body mass index [BMI] ≥ 30 kg/m² was collected. We used multivariable Poisson regression models to assess the association between general and central obesity and tooth loss after controlling for confounders. We also performed simple and multiple linear regressions by using BMI and WC as continuous variables. Interaction between age and tooth loss was also assessed. RESULTS: The mean BMI was 25.9 kg/m² (95%CI 25.6;26.2 in men and 25.4 kg/m2 (95%CI 25.0;25.7 in women. The mean WC was 79.3 cm (95%CI 78.4;80.1 in men and 88.4 cm (95%CI 87.6;89.2 in women. A positive association was found between the presence of less than 10 teeth in at least one arch and increased mean BMI and WC after adjusting for education level, self-reported diabetes, gender and monthly per capita income. However, this association was lost when the variable age was included in the model. The prevalence of general obesity was 50% higher in those with less than 10 teeth in at least one arch when compared with those with 10 or more teeth in both arches after adjusting for education level, self-reported diabetes and monthly per capita family income. However, the statistical significance was lost after controlling for age. CONCLUSIONS: Obesity was associated with number of teeth, though it depended on the participants' age groups.

  15. Population data of 16 autosomal STR loci of the Powerplex ESX 17 System in a Brazilian Population from the State of São Paulo.

    Science.gov (United States)

    Almeida Prado Oliveira e Sousa, Maria Luiza; de Oliveira, Marco Aurelio Tuena; Auler-Bittencout, Eloisa A; Soares-Vieira, Jose Arnaldo; Munoz, Daniel Romero; Iwamura, Edna Sadayo Miazato

    2014-07-01

    The State of São Paulo is the most populous state in Brazil, including approximately one fifth of the population of the country. In addition to a strong economy, the state has relatively good social indicators when compared with the rest of the country. The capital city, also called São Paulo, is the sixth largest city in the world. Its population is considered the most multicultural and racially mixed in Brazil. Currently, the largest populations in São Paulo are of Italian, Lebanese, Spanish and Japanese origin, and the state has the largest number of Northeasterners outside of the Northeast region. This population structure may lead to a particular genotype frequency. In this context, the formation of a new database containing the allele frequencies of five new genetic markers (D2S441, D10S1248, D22S1045, D1S1656 and D12S391) in a sample population is relevant. The allele frequencies of 16 STR loci, including the five new European Standard Set (ESS) loci, were calculated in a sample of 1088-1098 unrelated individuals, who geographically represent the Capital city.

  16. Sampling Lake Alice NWR Fish Populations - 2014, North Dakota

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The USFWS and North Dakota Game and Fish Department sampled Northern pike, walleye, yellow perch and white bass within the boundary of Lake Alice NWR in North...

  17. Translation, cultural adaptation and psychometric evaluation of the Leganés cognitive test in a low educated elderly Brazilian population

    Directory of Open Access Journals (Sweden)

    Vescia Vieira de Alencar Caldas

    2012-01-01

    Full Text Available OBJECTIVE: To validate the Leganés cognitive test (LCT for cognitive screening in low educated elderly Brazilians. METHODS: The study sample was composed of 59 elderly residents from the city of Santa Cruz, in Brazil, with low schooling levels. Reliability was analyzed with a two-day interval between assessments, and concurrent validity was assessed using the Mini Mental State Examination (MMSE. RESULTS: According to the LCT, the prevalence of dementia was 11.8%. The scale items showed a moderate to strong correlation between domains (p<0.01, and inter-rater reliability exhibited ICC=0.81, 95%CI=0.72-0.88. The factor analysis resulted in two factors: memory and orientation. Interscale agreement was considered poor (k=-0.02, supporting the hypothesis of an educational impact on final MMSE scores. CONCLUSION: The results suggest that LCT has acceptable levels of reliability for use in low-educated Brazilian elderly.

  18. Two Test Items to Explore High School Students' Beliefs of Sample Size When Sampling from Large Populations

    Science.gov (United States)

    Bill, Anthony; Henderson, Sally; Penman, John

    2010-01-01

    Two test items that examined high school students' beliefs of sample size for large populations using the context of opinion polls conducted prior to national and state elections were developed. A trial of the two items with 21 male and 33 female Year 9 students examined their naive understanding of sample size: over half of students chose a…

  19. Obsessive compulsive phenomenology in a sample of Egyptian adolescent population

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    Ahmed Rady

    2013-06-01

    Full Text Available Background and Objectives: Obsessive symptoms among children and adolescent age groups are increasing, an observation made by mental health professionals working with this age group. Our epidemiological study targeted secondary school students to estimate the prevalence of obsessive symptoms, obsessive compulsive disorder and their different obsessive compulsive contents. Methods: The study is cross sectional carried on 1299 secondary school students, the sample size was chosen based on an estimated Obsessive Compulsive Disorder (OCD prevalence of 2% in literature. Equal samples were recruited from the 3 educative zones in Alexandria Governorate. Obsessive compulsive symptoms were assessed by the Arabic version of Lyeton obsessive inventory child version LOI-CV. Students scoring above 35 were subjected to the Mini International Neuropsychiatric Interview for children MINI-KID Arabic. OCD patient students detected by MINI-KID were assessed by psychiatric interview to confirm fulfilling criteria of OCD according to DSM IV-TR criteria. Different obsessive compulsive symptoms were assessed by a standardized questionnaire. Results: Among the studied sample (n = 1299, 201 students scored > 35 on LOI-CV i.e. 15.5% of the total sample have OCS. The prevalence of OCD among studied sample was 2.2% as 29 students from the OCS students were fulfilling diagnostic criteria for OCD according to DSM-IV TR. Common obsessive symptoms were of excessive conscience 65.5%, blasphemous 55.2%, repeated words 51.7% and sexual obsessions 48.2%. Conclusions: The prevalence of obsessive compulsive symptoms is high among adolescent age group. Cultural impact should be considered to better understand obsessive phenomenology, raising the importance of OCD study from a transcultural perspective.

  20. The problem of sampling families rather than populations: Relatedness among individuals in samples of juvenile brown trout Salmo trutta L

    DEFF Research Database (Denmark)

    Hansen, Michael Møller; Eg Nielsen, Einar; Mensberg, Karen-Lise Dons

    1997-01-01

    In species exhibiting a nonrandom distribution of closely related individuals, sampling of a few families may lead to biased estimates of allele frequencies in populations. This problem was studied in two brown trout populations, based on analysis of mtDNA and microsatellites. In both samples mt......DNA haplotype frequencies differed significantly between age classes, and in one sample 17 out of 18 individuals less than 1 year of age shared one particular mtDNA haplotype. Estimates of relatedness showed that these individuals most likely represented only three full-sib families. Older trout exhibiting...

  1. A venue-based method for sampling hard-to-reach populations.

    Science.gov (United States)

    Muhib, F B; Lin, L S; Stueve, A; Miller, R L; Ford, W L; Johnson, W D; Smith, P J

    2001-01-01

    Constructing scientifically sound samples of hard-to-reach populations, also known as hidden populations, is a challenge for many research projects. Traditional sample survey methods, such as random sampling from telephone or mailing lists, can yield low numbers of eligible respondents while non-probability sampling introduces unknown biases. The authors describe a venue-based application of time-space sampling (TSS) that addresses the challenges of accessing hard-to-reach populations. The method entails identifying days and times when the target population gathers at specific venues, constructing a sampling frame of venue, day-time units (VDTs), randomly selecting and visiting VDTs (the primary sampling units), and systematically intercepting and collecting information from consenting members of the target population. This allows researchers to construct a sample with known properties, make statistical inference to the larger population of venue visitors, and theorize about the introduction of biases that may limit generalization of results to the target population. The authors describe their use of TSS in the ongoing Community Intervention Trial for Youth (CITY) project to generate a systematic sample of young men who have sex with men. The project is an ongoing community level HIV prevention intervention trial funded by the Centers for Disease Control and Prevention. The TSS method is reproducible and can be adapted to hard-to-reach populations in other situations, environments, and cultures.

  2. A melanoma risk score in a Brazilian population Um escore de risco para melanoma em uma população brasileira

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    Lucio Bakos

    2013-04-01

    Full Text Available BACKGROUND: Important risk factors for cutaneous melanoma (CM are recognized, but standardized scores for individual assessment must still be developed. OBJECTIVES: The objective of this study was to develop a risk score of CM for a Brazilian sample. METHODS: To verify the estimates of the main risk factors for melanoma, derived from a meta-analysis (Italian-based study, and externally validate them in a population in southern Brazil by means of a case-control study. A total of 117 individuals were evaluated. Different models were constructed combining the summary coefficients of different risk factors, derived from the meta-analysis, multiplied by the corresponding category of each variable for each participant according to a mathematical expression. RESULTS: the variable that best predicted the risk of CM in the studied population was hair color (AUC: 0.71; 95% CI: 0.62-0.79. Other important factors were freckles, sunburn episodes, and skin and eye color. Consideration of other variables such as common nevi, elastosis, family history, and premalignant lesions did not improve the predictive ability of the models. CONCLUSION: The discriminating capacity of the proposed model proved to be superior or comparable to that of previous risk models proposed for CM. FUNDAMENTOS: importantes fatores de risco para melanoma cutâneo são reconhecidos, mas escores padronizados para avaliação individual ainda precisam ser elaborados. OBJETIVOS: o objetivo deste estudo foi desenvolver um escore de risco de melanoma cutâneo para uma amostra brasileira. MÉTODOS: verificar as estimativas dos principais fatores de risco para melanoma, derivado de uma meta-análise (estudo de base italiano e, externamente, validar em uma população do sul do Brasil por um estudo caso-controle. Um total de 117 indivíduos foram avaliados. RESULTADOS: a variável com maior poder preditivo para o risco de melanoma cutâneo na população estudada foi a cor do cabelo (AUC: 0

  3. Ecological distribution and population structure of Acantholobulus schmitti (Rathbun, 1930 (Crustacea, Decapoda, Xanthoidea on the southeastern Brazilian coast

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    Vivian Fransozo

    2013-12-01

    Full Text Available This investigation analyzed the ecological distribution and population structure of A. schmitti on the southeastern coast of Brazil. Crabs were sampled monthly from January 1998 to December 1999 at the following bays: Ubatumirim (UBM, Ubatuba (UBA and Mar Virado (MV. Water and sediment samples were also collected from all sampling sites for an analysis of environmental factors. Acantholobus schmitti was most abundant at UBM (224, followed by UBA (154 and MV (23 but its abundance showed no association with the environmental factors analyzed. The low abundance of these crabs in MV may be due to the high wave action that moved biodetritic material accumulated on the bottom and frequently removed small crabs from their sheltered positions among the shell fragments. The individuals captured included 269 males and 132 females, of which only 4 specimens were brooding females. Juvenile recruitment occurred throughout the year, but was less intense in the spring. The major abundance of individuals as well as of ovigerous females occurred during 1999, when the entrance of the South Atlantic Central Waters (SACW was stronger than in previous year. This environmental influence could be the main factor modulating this population.

  4. Reductions in genetic diversity of Schistosoma mansoni populations under chemotherapeutic pressure: the effect of sampling approach and parasite population definition.

    Science.gov (United States)

    French, Michael D; Churcher, Thomas S; Basáñez, María-Gloria; Norton, Alice J; Lwambo, Nicholas J S; Webster, Joanne P

    2013-11-01

    Detecting potential changes in genetic diversity in schistosome populations following chemotherapy with praziquantel (PZQ) is crucial if we are to fully understand the impact of such chemotherapy with respect to the potential emergence of resistance and/or other evolutionary outcomes of interventions. Doing so by implementing effective, and cost-efficient sampling protocols will help to optimise time and financial resources, particularly relevant to a disease such as schistosomiasis currently reliant on a single available drug. Here we explore the effect on measures of parasite genetic diversity of applying various field sampling approaches, both in terms of the number of (human) hosts sampled and the number of transmission stages (miracidia) sampled per host for a Schistosoma mansoni population in Tanzania pre- and post-treatment with PZQ. In addition, we explore population structuring within and between hosts by comparing the estimates of genetic diversity obtained assuming a 'component population' approach with those using an 'infrapopulation' approach. We found that increasing the number of hosts sampled, rather than the number of miracidia per host, gives more robust estimates of genetic diversity. We also found statistically significant population structuring (using Wright's F-statistics) and significant differences in the measures of genetic diversity depending on the parasite population definition. The relative advantages, disadvantages and, hence, subsequent reliability of these metrics for parasites with complex life-cycles are discussed, both for the specific epidemiological and ecological scenario under study here and for their future application to other areas and schistosome species.

  5. Brazilian sandy beaches: characteristics, ecosystem services, impacts, knowledge and priorities

    Directory of Open Access Journals (Sweden)

    Antonia Cecília Zacagnini Amaral

    Full Text Available ABSTRACT Sandy beaches constitute a key ecosystem and provide socioeconomic goods and services, thereby playing an important role in the maintenance of human populations and in biodiversity conservation. Despite the ecological and social importance of these ecosytems, Brazilian sandy beaches are significantly impacted by human interference, chemical and organic pollution and tourism, as well as global climate change. These factors drive the need to better understand the environmental change and its consequences for biota. To promote the implementation of integrated studies to detect the effects of regional and global environmental change on beaches and on other benthic habitats of the Brazilian coast, Brazilian marine researchers have established The Coastal Benthic Habitats Monitoring Network (ReBentos. In order to provide input for sample planning by ReBentos, we have conducted an intensive review of the studies conducted on Brazilian beaches and summarized the current knowledge about this environment. In this paper, we present the results of this review and describe the physical, biological and socioeconomics features of Brazilian beaches. We have used these results, our personal experience and worldwide literature to identify research projects that should be prioritized in the assessment of regional and global change on Brazilian sandy beaches. We trust that this paper will provide insights for future studies and represent a significant step towards the conservation of Brazilian beaches and their biodiversity.

  6. Impact of Malocclusion on Oral Health-Related Quality of Life among Brazilian Preschool Children: a Population-Based Study

    OpenAIRE

    Carvalho, Anita Cruz; Saul Martins PAIVA; Viegas,Claudia Marina; Scarpelli,Ana Carolina; Ferreira, Fernanda de Morais; Pordeus, Isabela Almeida

    2013-01-01

    The purpose of the present study was to evaluate the impact of malocclusion on Oral Health-Related Quality of Life (OHRQoL) of children and their families. A population-based cross-sectional study was carried out in Belo Horizonte, MG, Brazil. A representative sample of 1069 male and female preschoolers aged 60 to 71 months was randomly selected from public and private preschools and daycare centers. Data were collected using the B-ECOHIS. In addition, a questionnaire addressing socioeconomic...

  7. Recruitment of hard-to-reach population subgroups via adaptations of the snowball sampling strategy.

    Science.gov (United States)

    Sadler, Georgia Robins; Lee, Hau-Chen; Lim, Rod Seung-Hwan; Fullerton, Judith

    2010-09-01

    Nurse researchers and educators often engage in outreach to narrowly defined populations. This article offers examples of how variations on the snowball sampling recruitment strategy can be applied in the creation of culturally appropriate, community-based information dissemination efforts related to recruitment to health education programs and research studies. Examples from the primary author's program of research are provided to demonstrate how adaptations of snowball sampling can be used effectively in the recruitment of members of traditionally underserved or vulnerable populations. The adaptation of snowball sampling techniques, as described in this article, helped the authors to gain access to each of the more-vulnerable population groups of interest. The use of culturally sensitive recruitment strategies is both appropriate and effective in enlisting the involvement of members of vulnerable populations. Adaptations of snowball sampling strategies should be considered when recruiting participants for education programs or for research studies when the recruitment of a population-based sample is not essential.

  8. Reference values for the cervical length measurement in the second trimester of pregnancy using the transvaginal ultrasound in a large Brazilian population

    Science.gov (United States)

    Peixoto, Alberto Borges; da Cunha Caldas, Taciana Mara Rodrigues; Alamy, Ana Helena Bittencourt; Martins, Wellington P.; Bruns, Rafael Frederico

    2016-01-01

    To establish reference values for the cervical length (CL) measurement by transvaginal ultrasound between 20 and 24+6 weeks of gestation in a large Brazilian population. A retrospective cross-sectional study was performed with 996 singleton pregnancies. The CL measurement (mm) using the transvaginal ultrasound was obtained in a sagittal view and the calipers positioned to measure the linear distance between the triangular area of echodensity at the external os and the internal os. The median±standard deviation and ranges for the CL measurement (mm) was 37.0±10.7 (range, 8 to 51). CL measurement did not modify significantly with gestational age. The observed percentiles for the CL measurement (mm) considering all number case were the following: 5th, 28 mm; 50th, 37 mm; and 95th, 45 mm. Reference values for the CL measurement by transvaginal ultrasound between 20 and 24+6 weeks of gestation in a large heterogeneous Brazilian population were established. PMID:27462597

  9. Brazilian Portuguese transcultural adaptation of Barkley Deficits in Executive Functioning Scale (BDEFS

    Directory of Open Access Journals (Sweden)

    Victor Polignano Godoy

    2015-12-01

    Full Text Available Abstract Background Considering the importance of Executive Functions to clinical and nonclinical situations, Barkley proposed a new theory of executive functioning based on an evolutionary neuropsychological perspective and clinical research using large samples of clinical and community identified adults and children as well as children with ADHD followed to adulthood. Objective The present study aims to adapt the Barkley Deficits in Executive Functions Scales (BDEFS to Brazilian Portuguese and also assess its construct validity in a sample of normal Brazilian adults. Methods The original version of scale was adapted to Brazilian Portuguese according to the guideline from the ISPOR Task Force. To assess the semantic equivalence between the original and adapted version, both of them were applied into a sample of 25 Brazilian bilingual adults. Finally, 60 Brazilian adults completed the BDEFS and the Brazilian versions of Barratt Impulsiveness Scale (BIS-11 and Adult Self-Report Scale (ASRS-18 to assess convergent validity. Results The BDEFS Brazilian Portuguese version has semantic correspondence with the original version indicating that the adaptation procedure was successful. The BDEFS correlated significantly with the impulsivity and attention scores from the BIS-11 and ASRS-18 supporting its construct validity. Cronbach’s alpha (α = 0.961 indicated that the BDEFS translated version has satisfactory internal consistency. Discussion Together, these findings indicate the successful adaptation of the BDEFS to Brazilian Portuguese and support its utility in that population.

  10. Level of Differentiation of Vocational Interests Profiles: Comparative Study by Age and Schooling in a Brazilian Sample

    Directory of Open Access Journals (Sweden)

    Ana Paula Porto Noronha

    2015-04-01

    Full Text Available Vocational interests can be defined as standards of preference, aversion or indifference to professional activities, but little is known about the factors involved in their development. From this perspective, this study attempted to clarify which variable, age or schooling, better fit comparisons of profile differentiation index. To this end, we analyzed the Escala de Aconselhamento Profissional (Professional Counseling Scale responses of 6,824 Brazilian students between 14 and 50 years old with various levels of education. Differentiation of the interest profile was observed by subtraction between dimensions with lower and higher scores. Normality of the distributions was verified and then Analysis of Variance and Tukey’s post hoc test were conducted in relation to groups of age and schooling. The results suggest that schooling is a more appropriate variable to compare the differentiation of interests. The implications and limitations of this study are discussed, and suggestions for future studies are given.

  11. A FAMILY OF ESTIMATORS FOR ESTIMATING POPULATION MEAN IN STRATIFIED SAMPLING UNDER NON-RESPONSE

    OpenAIRE

    Chaudhary, Manoj K.; RAJESH SINGH; Rakesh K. Shukla; MUKESH KUMAR; FLORENTIN SMARANDACHE

    2015-01-01

    Khoshnevisan et al. (2007) proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007) under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sampl...

  12. A FAMILY OF ESTIMATORS FOR ESTIMATING POPULATION MEAN IN STRATIFIED SAMPLING UNDER NON-RESPONSE

    OpenAIRE

    Chaudhary, Manoj K.; Rajesh Singh; Rakesh K. Shukla; Mukesh Kumar; Florentin Smarandache

    2009-01-01

    Khoshnevisan et al. (2007) proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007) under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sample sizes of the strata i...

  13. Estimativa da herdabilidade do diabetes juvenil em uma população brasileira An estimate of the inheritability of juvenile diabetes in a Brazilian population

    Directory of Open Access Journals (Sweden)

    Calógeras A. de A. Barbosa

    1984-10-01

    Full Text Available A herdabilidade da suscetibilidade do diabetes juvenil em uma população brasileira é estimada baseando-se em informações da prevalência da doença na população em geral e entre os pais de afetados. A estimativa de 90%, então obtida, sugere uma alta participação de fatores genéticos na determinação da moléstia. Exemplifica-se a aplicação desse resultado no cálculo de risco de recorrência do diabetes em familiares de indivíduos afetados.The inheritability of liability to juvenile diabetes in a Brazilian population is estimated from parent-offspring correlation. The high value of the estimate, 0.9, suggests that genetic factors are the most important causative agents of the disease in this population.

  14. Origin of the hemoglobin S gene in a northern Brazilian population: the combined effects of slave trade and internal migrations

    Directory of Open Access Journals (Sweden)

    Gabriella Pante-de-Sousa

    1998-12-01

    Full Text Available We analyzed DNA polymorphisms in the b-globin gene cluster of 30 sickle cell anemia patients from Belém, the capital city of the State of Pará, in order to investigate the origin of the bS mutation. Sixty-seven percent of the bS chromosomes were Bantu type, 30% were Benin type, and 3% were Senegal type. The origin of the bS mutation in this population, estimated on the basis of bS-linked haplotypes, contradicts the historical records of direct slave trade from Africa to the northern region of Brazil. Historical records indicate a lower percentage of people from Benin. These discrepancies are probably due to domestic slave trade and later internal migrations, mainly from northeastern to northern regions. Haplotype distribution in Belém did not differ significantly from that observed in other Brazilian regions, although historical records indicate that most slaves from Atlantic West Africa, where the Senegal haplotype is prevalent, were destined for the northern region, whereas the northeast (Bahia, Pernambuco and Maranhão was heavily supplied with slaves from Central West Africa, where the Benin haplotype predominates.Com o objetivo de investigar a origem da mutação bS na população da região norte do Brasil, foram analisados polimorfismos de DNA no complexo de genes b da hemoglobina em 30 pacientes com anemia falciforme na população de Belém, a capital do Estado do Pará. Sessenta e sete por cento dos cromossomos bS analisados apresentaram o haplótipo Bantu, 30% o haplótipo Benin e 3% o haplótipo Senegal. A origem da mutação bS na população de Belém, estimada de acordo com a distribuição de haplótipos, não está de acordo com a esperada com base em dados históricos sobre o tráfico de escravos para a região norte, os quais indicam uma reduzida contribuição de escravos da região do Benin. Essas diferenças podem ser atribuídas ao tráfico interno de escravos, bem como ao posterior fluxo de populações imigrantes

  15. Sustained Reduction of the Dengue Vector Population Resulting from an Integrated Control Strategy Applied in Two Brazilian Cities

    Science.gov (United States)

    Regis, Lêda N.; Acioli, Ridelane Veiga; Silveira, José Constantino; Melo-Santos, Maria Alice Varjal; Souza, Wayner Vieira; Ribeiro, Cândida M. Nogueira.; da Silva, Juliana C. Serafim.; Monteiro, Antonio Miguel Vieira; Oliveira, Cláudia M. F.; Barbosa, Rosângela M. R.; Braga, Cynthia; Rodrigues, Marco Aurélio Benedetti; Silva, Marilú Gomes N. M.; Ribeiro Jr., Paulo Justiniano; Bonat, Wagner Hugo; de Castro Medeiros, Liliam César; Carvalho, Marilia Sa; Furtado, André Freire

    2013-01-01

    Aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. Several trap models have been designed considering these strategies and tested for monitoring this efficient vector of Dengue. Here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. The SMCP-Aedes, a system based on open technology and open data standards, was set up from March/2008 to October/2011 as a pilot trial in two sites of Pernambuco -Brazil: Ipojuca (10,000 residents) and Santa Cruz (83,000), in a joint effort of health authorities and staff, and a network of scientists providing scientific support. A widespread infestation by Aedes was found in both sites in 2008–2009, with 96.8%–100% trap positivity. Egg densities were markedly higher in SCC than in Ipojuca. A 90% decrease in egg density was recorded in SCC after two years of sustained control pressure imposed by suppression of >7,500,000 eggs and >3,200 adults, plus larval control by adding fishes to cisterns. In Ipojuca, 1.1 million mosquito eggs were suppressed and a 77% reduction in egg density was achieved. This study aimed at assessing the applicability of a system using GIS and spatial statistic analysis tools for quantitative assessment of mosquito populations. It also provided useful information on the requirements for reducing well-established mosquito populations. Results from two cities led us to conclude that the success in markedly reducing an Aedes population required the appropriate choice of control measures for sustained mass elimination guided by a user-friendly mosquito surveillance system. The system was able to support interventional decisions and to assess the program’s success. Additionally, it created a stimulating environment for health staff and residents, which had a positive impact on their commitment to the dengue control program. PMID:23844059

  16. Sustained reduction of the dengue vector population resulting from an integrated control strategy applied in two Brazilian cities.

    Directory of Open Access Journals (Sweden)

    Lêda N Regis

    Full Text Available Aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. Several trap models have been designed considering these strategies and tested for monitoring this efficient vector of Dengue. Here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. The SMCP-Aedes, a system based on open technology and open data standards, was set up from March/2008 to October/2011 as a pilot trial in two sites of Pernambuco -Brazil: Ipojuca (10,000 residents and Santa Cruz (83,000, in a joint effort of health authorities and staff, and a network of scientists providing scientific support. A widespread infestation by Aedes was found in both sites in 2008-2009, with 96.8%-100% trap positivity. Egg densities were markedly higher in SCC than in Ipojuca. A 90% decrease in egg density was recorded in SCC after two years of sustained control pressure imposed by suppression of >7,500,000 eggs and >3,200 adults, plus larval control by adding fishes to cisterns. In Ipojuca, 1.1 million mosquito eggs were suppressed and a 77% reduction in egg density was achieved. This study aimed at assessing the applicability of a system using GIS and spatial statistic analysis tools for quantitative assessment of mosquito populations. It also provided useful information on the requirements for reducing well-established mosquito populations. Results from two cities led us to conclude that the success in markedly reducing an Aedes population required the appropriate choice of control measures for sustained mass elimination guided by a user-friendly mosquito surveillance system. The system was able to support interventional decisions and to assess the program's success. Additionally, it created a stimulating environment for health staff and residents, which had a positive impact on their commitment to the dengue control program.

  17. A Bayesian model for estimating population means using a link-tracing sampling design.

    Science.gov (United States)

    St Clair, Katherine; O'Connell, Daniel

    2012-03-01

    Link-tracing sampling designs can be used to study human populations that contain "hidden" groups who tend to be linked together by a common social trait. These links can be used to increase the sampling intensity of a hidden domain by tracing links from individuals selected in an initial wave of sampling to additional domain members. Chow and Thompson (2003, Survey Methodology 29, 197-205) derived a Bayesian model to estimate the size or proportion of individuals in the hidden population for certain link-tracing designs. We propose an addition to their model that will allow for the modeling of a quantitative response. We assess properties of our model using a constructed population and a real population of at-risk individuals, both of which contain two domains of hidden and nonhidden individuals. Our results show that our model can produce good point and interval estimates of the population mean and domain means when our population assumptions are satisfied.

  18. Prevalence of metabolic syndrome and its association with educational inequalities among Brazilian adults: a population-based study

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    M.F. Gronner

    2011-07-01

    Full Text Available The present study estimated the prevalence of metabolic syndrome (MS according to the criteria established by the National Cholesterol Education Program-Adult Treatment Panel III (NCEP-ATPIII and the International Diabetes Federation (IDF and analyzed the contribution of social factors in an adult urban population in the Southeastern region of Brazil. The sample plan was based on multistage probability sampling according to family head income and educational level. A random sample of 1116 subjects aged 30 to 79 years was studied. Participants answered a questionnaire about socio-demographic variables and medical history. Fasting capillary glucose (FCG, total cholesterol, high-density lipoprotein cholesterol (HDL-C, and triglycerides were determined and all non-diabetic subjects were submitted to the 75-g oral glucose tolerance test. Body mass index (BMI, kg/m², waist circumference and blood pressure (BP were determined. Age- and gender-adjusted prevalence of MS was 35.9 and 43.2% according to NCEP-ATPIII and IDF criteria, respectively. Substantial agreement was found between NCEP-ATPIII and IDF definitions. Low HDL-C levels and high BP were the most prevalent MS components according to NCEP-ATPIII criteria (76.3 and 59.2%, respectively. Considering the diagnostic criteria adopted, 13.5% of the subjects had diabetes and 9.7% had FCG ≥100 mg/dL. MS prevalence was significantly associated with age, skin color, BMI, and educational level. This cross-sectional population-based study in the Southeastern region of Brazil indicates that MS is highly prevalent and associated with an important social indicator, i.e., educational level. This result suggests that in developing countries health policy planning to reduce the risk of MS, in particular, should consider improvement in education.

  19. Quality of life among the Brazilian adult population using the generic SF-8 questionnaire Qualidade de vida da população adulta brasileira através do questionário SF-8

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    Alessandro Gonçalves Campolina

    2011-06-01

    Full Text Available The objective of this study was to assess the quality of life in the Brazilian adult population, based on the U.S. standard population. It involved a cross-sectional population-based study with probabilistic sampling of 2,420 individuals (725 men and 1695 women aged 40 or more in different geographic regions of Brazil. A socio-demographic questionnaire and the SF-8 (Short Form-8 were administered in interview form. Descriptive statistics, analysis of variance, the Mann-Whitney test and Tukey's test were used in the analysis. Females, populations in the northeastern region, the population of the regions of Brasília (Distrito Federal, Campo Grande (Mato Grosso do Sul State and Goiania (Goiás State, Brazil, demonstrated worse quality of life. Age, education and income had influence over quality of life domains. This study presents quality of life estimates for the Brazilian adult population, based on the SF-8 questionnaire. The mean values on the subscales and components of the SF-8 appeared to be influenced by gender, geographic region, family income, age and schooling.Avaliar a qualidade de vida da população adulta brasileira, com base em normas populacionais norte-americanas. Estudo transversal de base populacional, por amostragem probabilística. Dois mil, quatrocentos e vinte indivíduos (725 homens e 1.695 mulheres com idade de 40 anos ou mais foram avaliados em diferentes regiões geográficas brasileiras. Um questionário sociodemográfico e o SF-8 (Short Form-8 foram aplicados através de entrevista. Estatísticas descritivas, a análise de variância (ANOVA, o teste de Mann-Whitney e o teste de Tukey foram utilizados. O sexo feminino, a população da Região Nordeste e de Brasília (Distrito Federal, Goiânia (Goiás e Campo Grande (Mato Grosso do Sul apresentaram os piores níveis de qualidade de vida. A idade, a escolaridade e a renda familiar influenciaram negativamente vários domínios de qualidade de vida. O presente estudo

  20. Analysis of the CCR5 gene coding region diversity in five South American populations reveals two new non-synonymous alleles in Amerindians and high CCR5*D32 frequency in Euro-Brazilians

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    Angelica B.W. Boldt

    2009-01-01

    Full Text Available The CC chemokine receptor 5 (CCR5 molecule is an important co-receptor for HIV. The effect of the CCR5*D32 allele in susceptibility to HIV infection and AIDS disease is well known. Other alleles than CCR5*D32 have not been analysed before, neither in Amerindians nor in the majority of the populations all over the world. We investigated the distribution of the CCR5 coding region alleles in South Brazil and noticed a high CCR5*D32 frequency in the Euro-Brazilian population of the Paraná State (9.3%, which is the highest thus far reported for Latin America. The D32 frequency is even higher among the Euro-Brazilian Mennonites (14.2%. This allele is uncommon in Afro-Brazilians (2.0%, rare in the Guarani Amerindians (0.4% and absent in the Kaingang Amerindians and the Oriental-Brazilians. R223Q is common in the Oriental-Brazilians (7.7% and R60S in the Afro-Brazilians (5.0%. A29S and L55Q present an impaired response to b-chemokines and occurred in Afro- and Euro-Brazilians with cumulative frequencies of 4.4% and 2.7%, respectively. Two new non-synonymous alleles were found in Amerindians: C323F (g.3729G > T in Guarani (1.4% and Y68C (g.2964A > G in Kaingang (10.3%. The functional characteristics of these alleles should be defined and considered in epidemiological investigations about HIV-1 infection and AIDS incidence in Amerindian populations.

  1. Cross-cultural adaptation and psychometric properties of the Brazilian-Portuguese version of the VSP-A (Vécu et Santé Perçue de l'Adolescent, a health-related quality of life (HRQoL instrument for adolescents, in a healthy Brazilian population

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    Simeoni Marie-Claude

    2011-01-01

    Full Text Available Abstract Background Health-related quality of life (HRQoL assessment, encompassing the adolescents' perceptions of their mental, physical, and social health and well-being is increasingly considered an important outcome to be used to identify population health needs and to provide targeted medical care. Although validated instruments are essential for accurately assessing HRQoL outcomes, there are few cross-culturally adapted tools for use in Brazil, and none designed exclusively for use among adolescents. The Vécu et Santé Perçue de l'Adolescent (VSP-A is a generic, multidimensional self-reported instrument originally developed and validated in France that evaluates HRQoL of ill and healthy adolescents. Purpose To cross-culturally adapt and validate the Brazilian-Portuguese version of the VSP-A, a generic HRQoL measure for adolescents originally developed in France. Methods The VSP-A was translated following a well-validated forward-backward process leading to the Brazilian version. The psychometric evaluation was conducted in a sample of 446 adolescents (14-18 years attending 2 public high schools of São Gonçalo City. The adolescents self-reported the Brazilian VSP-A, the validated Psychosomatic Symptom Checklist and socio-demographic information. A retest evaluation was carried out on a sub-sample (n = 195 at a two-week interval. The internal construct validity was assessed through confirmatory factor analysis (CFA, multi-trait scaling analyses, Rasch analysis evaluating unidimensionality of each scale and Cronbach's alpha coefficients. The reproducibility was evaluated by intra-class correlation coefficients (ICC. Zumbo's ordinal logistic regression analysis was used to detect differential item functioning (DIF between the Brazilian and the French items. External construct validity was investigated testing expected differences between groups using one-way analysis of variance (ANOVA, Mann-Whitney tests and the univariate general

  2. Population genetic structure of the major malaria vector Anopheles darlingi (Diptera: Culicidae from the Brazilian Amazon, using microsatellite markers

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    Vera Margarete Scarpassa

    2007-06-01

    Full Text Available The population genetic structure of Anopheles darlingi, the major human malaria vector in the Neotropics, was examined using seven microsatellite loci from nine localities in central and western Amazonian Brazil. High levels of genetic variability were detected (5-25 alleles per locus; H E = 0.519-0.949. There was deviation from Hardy-Weinberg Equilibrium for 59.79% of the tests due to heterozygote deficits, while the analysis of linkage disequilibrium was significant for only two of 189 (1.05% tests, most likely caused by null alleles. Genetic differentiation (F ST = 0.001-0.095; Nm = 4.7-363.8 indicates that gene flow is extensive among locations < 152 km apart (with two exceptions and reduced, but not absent, at a larger geographic scale. Genetic and geographic distances were significantly correlated (R² = 0.893, P < 0.0002, supporting the isolation by distance (IBD model. The overall estimate of Ne was 202.4 individuals under the linkage disequilibrium model, and 8 under the heterozygote excess model. Analysis of molecular variance showed that nearly all variation (~ 94% was within sample locations. The UPGMA phenogram clustered the samples geographically, with one branch including 5/6 of the state of Amazonas localities and the other branch the Acre, Rondônia, and remaining Amazonas localities. Taken together, these data suggest little genetic structure for An. darlingi from central and western Amazonian Brazil. These findings also imply that the IBD model explains nearly all of the differentiation detected. In practical terms, populations of An. darlingi at distances < 152 km should respond similarly to vector control measures, because of high gene flow.

  3. Comparison of risk and protective factors for chronic diseases in the population with and without health insurance in the Brazilian capitals, 2011

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    Deborah Carvalho Malta

    2014-01-01

    Full Text Available INTRODUCTION: The article compares the risk and protective factors for Non-communicable Diseases (NCD, referred morbidity and access to preventive examinations in the population with and without health insurance in all Brazilian State capitals. METHODS: The study population consists of adults (≥ 18 years old living in households with landlines in 26 Brazilian State capitals and the Federal District. Estimates of selected variables are presented according to possession of health plans ("Yes" or "No" and sex. A post-stratification was performed according to age, gender and education in both populations, and prevalence ratios were calculated, adjusted for age and sex between people with and without health insurance for the risk and protective factors for NCDs. RESULTS: A total of 54,099 people at the age of 18 or older were evaluated, 47.4% of them were beneficiaries of health plans. The coverage of health insurance tends to increase with age and level of education. Compared to non-beneficiaries of health plans, beneficiaries were more likely to have protective factors, such as healthy eating, physical activity, coverage tests, such as mammography and Pap test, and lower prevalence of risk factors such as smoking, physical inactivity, poor health assessment and hypertension. Alcohol abuse, consumption of excessively fat meats, overweight, obesity and diabetes were not associated with the variable possession of health insurance. When controlled by education, individuals who have health insurance generally have better indicators. CONCLUSION: This information is important to establish measures for reducing differences among people with and without health insurance.

  4. Condições periodontais e diabetes mellitus na população nipo-brasileira Periodontal conditions and diabetes mellitus in the Japanese-Brazilian population

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    Nilce E Tomita

    2002-10-01

    Full Text Available OBJETIVO: Avaliar as condições periodontais e sua relação com o diabetes mellitus na população nipo-brasileira. MÉTODOS: Foram examinados 1.315 indivíduos do município de Bauru, SP, na faixa etária de 30 a 92 anos de idade, ambos os sexos, primeira (Isseis e segunda (Niseis gerações. Os critérios de exclusão da amostra foram o edentulismo total e a presença de seis sextantes nulos. O índice periodontal comunitário e o índice de perda de inserção periodontal foram obtidos mediante sondagem em 10 dentes-índice, em uma amostra de 831 indivíduos. O diagnóstico de diabetes mellitus foi estabelecido através da glicemia em jejum e de duas horas após sobrecarga com 75 g de glicose. Para análise estatística foram utilizados os Testes de Kappa e de Qui-quadrado. RESULTADOS: Quanto às condições periodontais, foram encontrados 25,5% de indivíduos sadios, 12,5% com sangramento à sondagem, 49,4% com presença de cálculo, 10,4% com bolsas superficiais, 2,2% com bolsas profundas. Apresentaram perdas de inserção periodontal de 0-3 mm, 24,2% dos indivíduos, de 4-5 mm, 36,7%, de 6-8 mm, 23,7%, de 9-11 mm, 11,3% e de 12 mm ou mais, 4,1%. A avaliação entre diabetes e condições periodontais não apresentou associação estatística (pOBJECTIVE: To evaluate the relationship between diabetic status and periodontal conditions in the Japanese-Brazilian population. METHODS: The sample consisted of 1,315 subjects, of both sexes, first (Issei and second (Nisei generations, aged 30 to 92 years, living in Bauru, Brazil. Edentulism and presence of 6 null sextants were the exclusion criteria for the sample. The Community Periodontal Index and Periodontal Attachment Loss Index were determined using the probing of 10 teeth in a sample of 831 subjects. The diagnosis of diabetes mellitus was based on fasting blood sugar and blood sugar 2 hours after 75 mg of glucose overload. Statistical analysis was conducted using Kappa test and Chi

  5. Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest.

    Science.gov (United States)

    Gonçalves-Machado, Larissa; Pla, Davinia; Sanz, Libia; Jorge, Roberta Jeane B; Leitão-De-Araújo, Moema; Alves, Maria Lúcia M; Alvares, Diego Janisch; De Miranda, Joari; Nowatzki, Jenifer; de Morais-Zani, Karen; Fernandes, Wilson; Tanaka-Azevedo, Anita Mitico; Fernández, Julián; Zingali, Russolina B; Gutiérrez, José María; Corrêa-Netto, Carlos; Calvete, Juan J

    2016-03-01

    Bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, it is often abundant and is an important cause of snakebite. Although no subspecies are currently recognized, geographic analyses have revealed the existence of two well-supported B. jararaca clades that diverged during the Pliocene ~3.8Mya and currently display a southeastern (SE) and a southern (S) Atlantic rainforest (Mata Atlântica) distribution. The spectrum, geographic variability, and ontogenetic changes of the venom proteomes of snakes from these two B. jararaca phylogroups were investigated applying a combined venom gland transcriptomic and venomic analysis. Comparisons of the venom proteomes and transcriptomes of B. jararaca from the SE and S geographic regions revealed notable interpopulational variability that may be due to the different levels of population-specific transcriptional regulation, including, in the case of the southern population, a marked ontogenetic venom compositional change involving the upregulation of the myotoxic PLA2 homolog, bothropstoxin-I. This population-specific marker can be used to estimate the proportion of venom from the southern population present in the B. jararaca venom pool used for the Brazilian soro antibotrópico (SAB) antivenom production. On the other hand, the southeastern population-specific D49-PLA2 molecules, BinTX-I and BinTX-II, lend support to the notion that the mainland ancestor of Bothrops insularis was originated within the same population that gave rise to the current SE B. jararaca phylogroup, and that this insular species endemic to Queimada Grande Island (Brazil) expresses a pedomorphic venom phenotype. Mirroring their compositional divergence, the two geographic B. jararaca venom pools showed distinct bioactivity profiles. However, the SAB antivenom manufactured in Vital Brazil

  6. Aptidão cardiorrespiratória de uma amostra regional brasileira distribuída em diferentes tabelas Cardiorespiratory fitness of a Brazilian regional sample distributed in different tables

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    Karlyse Claudino Belli

    2012-09-01

    Full Text Available FUNDAMENTO: A maioria das tabelas de classificação da Aptidão Cardiorrespiratória (ACR utilizadas na prática clínica é internacional e não foi validada para a população brasileira, podendo resultar em discrepâncias importantes, uma vez que essa classificação é extrapolada para a nossa população. Objetivo: Avaliar as principais tabelas de ACR disponíveis em uma amostra populacional brasileira do Planalto Médio do Rio Grande do Sul (RS. MÉTODOS: Foram analisados dados retrospectivos de 2.930 indivíduos, residentes em 36 cidades do Planalto Central do RS. Levaram-se em consideração presença dos fatores de risco para doença cardiovascular e valores estimados do consumo de oxigênio de pico (VO2pico, obtidos por meio de teste de esforço com protocolo de Bruce. Para classificar a ACR, os sujeitos foram distribuídos de acordo com o sexo e inseridos nas respectivas faixas etárias das tabelas de Cooper, American Heart Association (AHA e da Universidade Federal de São Paulo (Unifesp, e classificados conforme seu VO2pico. RESULTADOS: A amostra feminina apresentou valores mais baixos de VO2pico do que a masculina (23,5 ± 8,5 vs. 31,7 ± 10,8 mL.kg-1.min-1, p BACKGROUND: Most classification tables of cardiorespiratory fitness (CRF used in clinical practice are international and have not been validated for the Brazilian population. That can result in important discrepancies when that classification is extrapolated to our population. OBJECTIVE: To assess the use of major CRF tables available in a Brazilian population sample of the Central High Plan of the state of Rio Grande do Sul (RS. METHODS: This study assessed the retrospective data of 2,930 individuals, living in 36 cities of the Central High Plan of the state of RS, and considered the following: presence of risk factors for cardiovascular disease and estimated maximum oxygen consumption (VO2peak values obtained through exercise test with Bruce protocol. To classify CRF, the

  7. Stratified Sampling of Neighborhood Sections for Population Estimation: A Case Study of Bo City, Sierra Leone.

    Science.gov (United States)

    Hillson, Roger; Alejandre, Joel D; Jacobsen, Kathryn H; Ansumana, Rashid; Bockarie, Alfred S; Bangura, Umaru; Lamin, Joseph M; Stenger, David A

    2015-01-01

    There is a need for better estimators of population size in places that have undergone rapid growth and where collection of census data is difficult. We explored simulated estimates of urban population based on survey data from Bo, Sierra Leone, using two approaches: (1) stratified sampling from across 20 neighborhoods and (2) stratified single-stage cluster sampling of only four randomly-sampled neighborhoods. The stratification variables evaluated were (a) occupants per individual residence, (b) occupants per neighborhood, and (c) residential structures per neighborhood. For method (1), stratification variable (a) yielded the most accurate re-estimate of the current total population. Stratification variable (c), which can be estimated from aerial photography and zoning type verification, and variable (b), which could be ascertained by surveying a limited number of households, increased the accuracy of method (2). Small household-level surveys with appropriate sampling methods can yield reasonably accurate estimations of urban populations.

  8. Population survey sampling methods in a rural African setting: measuring mortality

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    Byass Peter

    2008-05-01

    Full Text Available Abstract Background Population-based sample surveys and sentinel surveillance methods are commonly used as substitutes for more widespread health and demographic monitoring and intervention studies in resource-poor settings. Such methods have been criticised as only being worthwhile if the results can be extrapolated to the surrounding 100-fold population. With an emphasis on measuring mortality, this study explores the extent to which choice of sampling method affects the representativeness of 1% sample data in relation to various demographic and health parameters in a rural, developing-country setting. Methods Data from a large community based census and health survey conducted in rural Burkina Faso were used as a basis for modelling. Twenty 1% samples incorporating a range of health and demographic parameters were drawn at random from the overall dataset for each of seven different sampling procedures at two different levels of local administrative units. Each sample was compared with the overall 'gold standard' survey results, thus enabling comparisons between the different sampling procedures. Results All sampling methods and parameters tested performed reasonably well in representing the overall population. Nevertheless, a degree of variation could be observed both between sampling approaches and between different parameters, relating to their overall distribution in the total population. Conclusion Sample surveys are able to provide useful demographic and health profiles of local populations. However, various parameters being measured and their distribution within the sampling unit of interest may not all be best represented by a particular sampling method. It is likely therefore that compromises may have to be made in choosing a sampling strategy, with costs, logistics the intended use of the data being important considerations.

  9. Eating patterns in a population-based sample of children aged 5 to 7 years

    DEFF Research Database (Denmark)

    Micali, Nadia; Simonoff, Emily; Elberling, Hanne

    2011-01-01

    There is still a lack of research on childhood eating patterns and their correlates in relation to psychopathology and parentally perceived impact in general population samples. We aimed to determine which eating patterns were more likely to be identified as problematic by parents, and their impact...... and association with childhood psychopathology (emotional, behavioral, and pervasive developmental disorders) in a general population child cohort....

  10. Population and Family Education. Book II. Draft Sample Instructional Materials. Social Studies.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    Produced by participants at the Unesco Regional workshop on Population and Family Education held in Bangkok, Thailand, in October 1970, the instructional materials intended for elementary and secondary students are to be considered sample first-draft materials usable for reference purposes by groups responsible for designing population education…

  11. The positive effects of population-based preferential sampling in environmental epidemiology.

    Science.gov (United States)

    Antonelli, Joseph; Cefalu, Matthew; Bornn, Luke

    2016-10-01

    SummaryIn environmental epidemiology, exposures are not always available at subject locations and must be predicted using monitoring data. The monitor locations are often outside the control of researchers, and previous studies have shown that "preferential sampling" of monitoring locations can adversely affect exposure prediction and subsequent health effect estimation. We adopt a slightly different definition of preferential sampling than is typically seen in the literature, which we call population-based preferential sampling. Population-based preferential sampling occurs when the location of the monitors is dependent on the subject locations. We show the impact that population-based preferential sampling has on exposure prediction and health effect estimation using analytic results and a simulation study. A simple, one-parameter model is proposed to measure the degree to which monitors are preferentially sampled with respect to population density. We then discuss these concepts in the context of PM2.5 and the EPA Air Quality System monitoring sites, which are generally placed in areas of higher population density to capture the population's exposure.

  12. Green Ocean Amazon 2014/15 High-Volume Filter Sampling: Atmospheric Particulate Matter of an Amazon Tropical City and its Relationship to Population Health Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Machado, C. M. [Federal Univ. of Amazonas (Brazil); Santos, Erickson O. [Federal Univ. of Amazonas (Brazil); Fernandes, Karenn S. [Federal Univ. of Amazonas (Brazil); Neto, J. L. [Federal Univ. of Amazonas (Brazil); Souza, Rodrigo A. [Univ. of the State of Amazonas (Brazil)

    2016-08-01

    Manaus, the capital of the Brazilian state of Amazonas, is developing very rapidly. Its pollution plume contains aerosols from fossil fuel combustion mainly due to vehicular emission, industrial activity, and a thermal power plant. Soil resuspension is probably a secondary source of atmospheric particles. The plume transports from Manaus to the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility ARM site at Manacapuru urban pollutants as well as pollutants from pottery factories along the route of the plume. Considering the effects of particulate matter on health, atmospheric particulate matter was evaluated at this site as part of the ARM Facility’s Green Ocean Amazon 2014/15 (GoAmazon 2014/15) field campaign. Aerosol or particulate matter (PM) is typically defined by size, with the smaller particles having more health impact. Total suspended particulate (TSP) are particles smaller than 100 μm; particles smaller than 2.5 μm are called PM2.5. In this work, the PM2.5 levels were obtained from March to December of 2015, totaling 34 samples and TSP levels from October to December of 2015, totaling 17 samples. Sampling was conducted with PM2.5 and TSP high-volume samplers using quartz filters (Figure 1). Filters were stored during 24 hours in a room with temperature (21,1ºC) and humidity (44,3 %) control, in order to do gravimetric analyses by weighing before and after sampling. This procedure followed the recommendations of the Brazilian Association for Technical Standards local norm (NBR 9547:1997). Mass concentrations of particulate matter were obtained from the ratio between the weighted sample and the volume of air collected. Defining a relationship between particulate matter (PM2.5 and TSP) and respiratory diseases of the local population is an important goal of this project, since no information exists on that topic.

  13. Sampling Methods and the Accredited Population in Athletic Training Education Research

    Science.gov (United States)

    Carr, W. David; Volberding, Jennifer

    2009-01-01

    Context: We describe methods of sampling the widely-studied, yet poorly defined, population of accredited athletic training education programs (ATEPs). Objective: There are two purposes to this study; first to describe the incidence and types of sampling methods used in athletic training education research, and second to clearly define the…

  14. Improved sampling for airborne surveys to estimate wildlife population parameters in the African Savannah

    NARCIS (Netherlands)

    Khaemba, W.; Stein, A.

    2002-01-01

    Parameter estimates, obtained from airborne surveys of wildlife populations, often have large bias and large standard errors. Sampling error is one of the major causes of this imprecision and the occurrence of many animals in herds violates the common assumptions in traditional sampling designs like

  15. Semiparametric efficient and robust estimation of an unknown symmetric population under arbitrary sample selection bias

    KAUST Repository

    Ma, Yanyuan

    2013-09-01

    We propose semiparametric methods to estimate the center and shape of a symmetric population when a representative sample of the population is unavailable due to selection bias. We allow an arbitrary sample selection mechanism determined by the data collection procedure, and we do not impose any parametric form on the population distribution. Under this general framework, we construct a family of consistent estimators of the center that is robust to population model misspecification, and we identify the efficient member that reaches the minimum possible estimation variance. The asymptotic properties and finite sample performance of the estimation and inference procedures are illustrated through theoretical analysis and simulations. A data example is also provided to illustrate the usefulness of the methods in practice. © 2013 American Statistical Association.

  16. Composition of shrimp populations (Crustacea: Decapoda in non-vegetated areas of two river islands in a Brazilian Amazon estuary

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    Priscila Sousa Vilela da Nóbrega

    2013-12-01

    Full Text Available This study investigates the shrimp found in non-vegetated areas of an estuary of the Amazon River. We ascertained the input of juveniles, species' biometrics and the influence of environmental factors on the abundance of species. The samples were collected monthly, from August 2006 to July 2007, in two places in the estuary, each next to an island. For collecting, we used a manual trawl to perform three hauls per month, totaling 36 samples per site. The abundance of shrimps was estimated as a function of the density of specimens per unit area. We used the Spearman's correlation to test the hypothesis that there is significant correlation between the average of the environmental variables measured and variations in shrimp density. The Kruskal-Wallis and the Mann-Whitney tests showed that there were significant differences in environment factors (temperature and salinity among the months and sites. We obtained 6,091 shrimps, from which 5,231 (85.88% were caught off the Arapiranga Island and 860 (14.12% off the Mosqueiro Island, Palaemonidae and Penaeidae were the only families recorded. Five species were collected: Macrobrachium amazonicum (Heller, 1862, Macrobrachium surinamicum Holthuis, 1948, Macrobrachium carcinus (Linnaeus, 1758, Macrobrachium rosenbergii (De Man, 1879, and Farfantepenaeus subtilis (Pérez-Farfante, 1967. The latter (pink shrimp was found for the first time in oligohaline environments (0-8. Macrobrachium amazonicum was the most abundant species. The recruitment of M. amazonicum juveniles was continuous throughout the year. The population of M. surinamicum was composed by juveniles and adults and that of F. subtilis exclusively by juveniles. The environmental factors analyzed were variable throughout the year and seem to explain the patterns of shrimp species occurrence in the region, the variation in their abundance and juvenile recruitment.

  17. Impact of malocclusion on oral health-related quality of life among Brazilian preschool children: a population-based study.

    Science.gov (United States)

    Carvalho, Anita Cruz; Paiva, Saul Martins; Viegas, Claudia Marina; Scarpelli, Ana Carolina; Ferreira, Fernanda Morais; Pordeus, Isabela Almeida

    2013-01-01

    The purpose of the present study was to evaluate the impact of malocclusion on Oral Health-Related Quality of Life (OHRQoL) of children and their families. A population-based cross-sectional study was carried out in Belo Horizonte, MG, Brazil. A representative sample of 1069 male and female preschoolers aged 60 to 71 months was randomly selected from public and private preschools and daycare centers. Data were collected using the B-ECOHIS. In addition, a questionnaire addressing socioeconomic and demographic data was self-administered by the parents/guardians. The criteria used to diagnose malocclusion were based on Foster and Hamilton (1969), Graboswki et al. (2007) and Oliveira et al. (2008). Descriptive, univariate and multiple Poison logistic regression analyses were carried out. The prevalence of malocclusion was observed in 46.2% of the children and deep overbite was the most prevalent type of malocclusion (19.7%), followed by posterior crossbite (13.1%), accentuated overjet (10.5%), anterior open bite (7.9%) and anterior crossbite (6.7%). The impact of malocclusion on OHRQoL was 32.7% among the children and 27.1% among the families. In Poisson multiple regression model adjusted for socioeconomic status, no significant association was found between malocclusion and OHRQoL of the children (PR=1.09, 95% CI: 0.96-1.24) and their families (PR=1.11, 95% CI: 0.94-1.31). It is concluded that children with malocclusion in this sample did not have a negative impact on their OHRQoL and of their families.

  18. Estimating variable effective population sizes from multiple genomes: a sequentially markov conditional sampling distribution approach.

    Science.gov (United States)

    Sheehan, Sara; Harris, Kelley; Song, Yun S

    2013-07-01

    Throughout history, the population size of modern humans has varied considerably due to changes in environment, culture, and technology. More accurate estimates of population size changes, and when they occurred, should provide a clearer picture of human colonization history and help remove confounding effects from natural selection inference. Demography influences the pattern of genetic variation in a population, and thus genomic data of multiple individuals sampled from one or more present-day populations contain valuable information about the past demographic history. Recently, Li and Durbin developed a coalescent-based hidden Markov model, called the pairwise sequentially Markovian coalescent (PSMC), for a pair of chromosomes (or one diploid individual) to estimate past population sizes. This is an efficient, useful approach, but its accuracy in the very recent past is hampered by the fact that, because of the small sample size, only few coalescence events occur in that period. Multiple genomes from the same population contain more information about the recent past, but are also more computationally challenging to study jointly in a coalescent framework. Here, we present a new coalescent-based method that can efficiently infer population size changes from multiple genomes, providing access to a new store of information about the recent past. Our work generalizes the recently developed sequentially Markov conditional sampling distribution framework, which provides an accurate approximation of the probability of observing a newly sampled haplotype given a set of previously sampled haplotypes. Simulation results demonstrate that we can accurately reconstruct the true population histories, with a significant improvement over the PSMC in the recent past. We apply our method, called diCal, to the genomes of multiple human individuals of European and African ancestry to obtain a detailed population size change history during recent times.

  19. Development of a novel cell sorting method that samples population diversity in flow cytometry.

    Science.gov (United States)

    Osborne, Geoffrey W; Andersen, Stacey B; Battye, Francis L

    2015-11-01

    Flow cytometry based electrostatic cell sorting is an important tool in the separation of cell populations. Existing instruments can sort single cells into multi-well collection plates, and keep track of cell of origin and sorted well location. However currently single sorted cell results reflect the population distribution and fail to capture the population diversity. Software was designed that implements a novel sorting approach, "Slice and Dice Sorting," that links a graphical representation of a multi-well plate to logic that ensures that single cells are sampled and sorted from all areas defined by the sort region/s. Therefore the diversity of the total population is captured, and the more frequently occurring or rarer cell types are all sampled. The sorting approach was tested computationally, and using functional cell based assays. Computationally we demonstrate that conventional single cell sorting can sample as little as 50% of the population diversity dependant on the population distribution, and that Slice and Dice sorting samples much more of the variety present within a cell population. We then show by sorting single cells into wells using the Slice and Dice sorting method that there are cells sorted using this method that would be either rarely sorted, or not sorted at all using conventional single cell sorting approaches. The present study demonstrates a novel single cell sorting method that samples much more of the population diversity than current methods. It has implications in clonal selection, stem cell sorting, single cell sequencing and any areas where population heterogeneity is of importance.

  20. Genotyping faecal samples of Bengal tiger Panthera tigris tigris for population estimation: A pilot study

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    Singh Lalji

    2006-10-01

    Full Text Available Abstract Background Bengal tiger Panthera tigris tigris the National Animal of India, is an endangered species. Estimating populations for such species is the main objective for designing conservation measures and for evaluating those that are already in place. Due to the tiger's cryptic and secretive behaviour, it is not possible to enumerate and monitor its populations through direct observations; instead indirect methods have always been used for studying tigers in the wild. DNA methods based on non-invasive sampling have not been attempted so far for tiger population studies in India. We describe here a pilot study using DNA extracted from faecal samples of tigers for the purpose of population estimation. Results In this study, PCR primers were developed based on tiger-specific variations in the mitochondrial cytochrome b for reliably identifying tiger faecal samples from those of sympatric carnivores. Microsatellite markers were developed for the identification of individual tigers with a sibling Probability of Identity of 0.005 that can distinguish even closely related individuals with 99.9% certainty. The effectiveness of using field-collected tiger faecal samples for DNA analysis was evaluated by sampling, identification and subsequently genotyping samples from two protected areas in southern India. Conclusion Our results demonstrate the feasibility of using tiger faecal matter as a potential source of DNA for population estimation of tigers in protected areas in India in addition to the methods currently in use.

  1. An alternative procedure for estimating the population mean in simple random sampling

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    Housila P. Singh

    2012-03-01

    Full Text Available This paper deals with the problem of estimating the finite population mean using auxiliary information in simple random sampling. Firstly we have suggested a correction to the mean squared error of the estimator proposed by Gupta and Shabbir [On improvement in estimating the population mean in simple random sampling. Jour. Appl. Statist. 35(5 (2008, pp. 559-566]. Later we have proposed a ratio type estimator and its properties are studied in simple random sampling. Numerically we have shown that the proposed class of estimators is more efficient than different known estimators including Gupta and Shabbir (2008 estimator.

  2. A FAMILY OF ESTIMATORS FOR ESTIMATING POPULATION MEAN IN STRATIFIED SAMPLING UNDER NON-RESPONSE

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    Manoj K. Chaudhary

    2009-01-01

    Full Text Available Khoshnevisan et al. (2007 proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007 under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sample sizes of the strata in respect to cost of the survey. Results are also supported by numerical analysis.

  3. Associations between dietary patterns and self-reported hypertension among Brazilian adults: a cross-sectional population-based study.

    Science.gov (United States)

    Selem, Soraya Sant'Ana de Castro; Castro, Michelle Alessandra de; César, Chester Luiz Galvão; Marchioni, Dirce Maria Lobo; Fisberg, Regina Mara

    2014-08-01

    Hypertension is a prevalent cardiovascular disease, the important modifiable risk factor of which is diet. The aim of this study was to derive dietary patterns and to test associations with self-reported hypertension and other characteristics, namely demographic, socioeconomic, and lifestyle factors. Data were obtained from the population-based cross-sectional study titled Health Survey of the City of São Paulo, with a random sample of residents of the city of São Paulo, Brazil, aged older than 20 years of both sexes (n=1,102). In 2008, a structured questionnaire with information about socioeconomic, anthropometric, lifestyle, and dietary factors was applied. Dietary intake was estimated by two 24-hour dietary recalls, adjusted by Multiple Source Method. Dietary patterns were obtained through exploratory principal component factor analysis. Poisson regression was used to assess relationships. Three dietary patterns were identified: prudent (fruits, vegetables, whole-grain bread, white cheeses, juices, reduced-fat milk/nonfat milk), traditional (rice, beans, bread/toast/crackers, butter/margarine, whole milk, coffee/teas, sugar), and modern (sodas, pastries/sandwiches/pizzas, yellow cheeses, pastas, sauces, alcoholic beverages, sweets, processed meats). Hypertension and demographic, socioeconomic, and lifestyle factors, as well as the presence of health insurance, were associated with adherence to one or more identified dietary patterns. These results suggest the existence of a target audience for planning and executing public policies of food and nutrition to prevent and control hypertension.

  4. Temperature-dependent development and survival of Brazilian populations of the Mediterranean fruit fly, Ceratitis capitata, from tropical, subtropical and temperate regions.

    Science.gov (United States)

    Ricalde, Marcelo P; Nava, Dori E; Loeck, Alci E; Donatti, Michele G

    2012-01-01

    The Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is one of the principal exotic pests affecting Brazilian production in the northeastern and southeastern regions of Brazil. In the south, it is has potential as a serious threat to temperate-climate fruit farms, since it is already found in urban and suburban communities in this region. We studied the biological characteristics of C. capitata populations from Pelotas-RS (temperate climate), Petrolina-PE (tropical), and Campinas-SP (subtropical). Ceratitis capitata biology was studied under controlled temperature (15, 20, 25, 30, and 35 ± 1 °C), 70 ± 10% RH, and 14:10 L:D photoperiod. The duration and survival rate of the egg, larval, and pupal stages were evaluated and the thermal requirements of these three populations were determined. The duration and survival of these developmental stages varied with temperature, with similar values for the three populations, except for some variation in the egg phase. Egg to adult developmental time for all three populations was inversely proportional to temperature; from 15 to 30 °C developmental time varied from 71.2 to 17.1, 70.2 to 17.1, and 68.5 to 16.9 days, respectively. Survival during development was affected at 15 to 30 °C, and differed significantly from survival at 20 to 25 °C. At 35 °C, immature stages did not develop. The basal temperature and degree-day requirement were similar for all immature stages except for the egg stage. The basal temperatures and thermal constants were 9.30 and 350, 8.47 and 341, and 9.60 °C and 328 degree-days for the Pelotas, Petrolina, and Campinas populations, respectively. Results suggested that survival and thermal requirements are similar for these tropical, subtropical, and temperate populations of C. capitata, and demonstrate the species' capacity to adapt to different climate conditions.

  5. Inorganic profile of some Brazilian medicinal plants obtained from ethanolic extract and ''in natura'' samples

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, M.O.M.; de Sousa, P.T.; Salvador, V.L.R.; Sato, I.M.

    2004-10-03

    The Anadenathera macrocarpa, Schinus molle, Hymenaea courbaril, Cariniana legalis, Solidago microglossa and Stryphnodendron barbatiman, were collected ''in natura'' samples (leaves, flowers, barks and seeds) from different commercial suppliers. The pharmaco-active compounds in ethanolic extracts had been made by the Mato Grosso Federal University (UFMT). The energy-dispersive x-ray fluorescence (ED-XRF) spectrometry was used for the elemental analysis in different parts of the plants and respective ethanolic extracts. The Ca, Cl, Cu, Fe, K, Mg, Mn, Na, Ni, P, Rb, S, Sr and Zn concentrations were determined by the fundamental parameters method. Some specimens showed a similar inorganic profile for ''in natura'' and ethanolic extract samples and some ones showed a distinct inorganic profile. For example, the Anadenathera macrocarpa showed a similar concentration in Mg, P, Cu, Zn and Rb elements in ''in natura'' and ethanolic extract samples; however very different concentration in Na, S, Cl, K , Ca, Mn, Fe and Sr was observed in distinctive samples. The Solidago microglossa showed the K, Ca, Cl, S, Mg, P and Fe elements as major constituents in both samples, suggesting that the extraction process did not affect in a considerable way the ''in natura'' inorganic composition. The elemental composition of the different parts of the plants (leaves, flowers, barks and seeds) has been also determined. For example, the Schinus molle specimen showed P, K, Cl and Ca elements as major constituents in the seeds, Mg, K and Sr in the barks and Mg, S, Cl and Mn in the leaves, demonstrating a differentiated elementary distribution. These inorganic profiles will contribute to evaluate the quality control of the Brazilian herbaceous trade and also will assist to identify which parts of the medicinal plants has greater therapeutic effect.

  6. Genetic diversity of Brazilian natural populations of Anthonomus grandis Boheman (Coleoptera: Curculionidae), the major cotton pest in the New World.

    Science.gov (United States)

    Martins, W F S; Ayres, C F J; Lucena, W A

    2007-01-01

    Twenty-five RAPD loci and 6 isozyme loci were studied to characterize the genetic variability of natural populations of Anthonomus grandis from two agroecosystems of Brazil. The random-amplified polymorphic DNA data disclosed a polymorphism that varied from 52 to 84% and a heterozygosity of 0.189 to 0.347. The index of genetic differentiation (GST) among the six populations was 0.258. The analysis of isozymes showed a polymorphism and a heterozygosity ranging from 25 to 100% and 0.174 to 0.277, respectively. The genetic differentiation (FST) among the populations obtained by isozyme data was 0.544. It was possible to observe rare alleles in the populations from the Northeast region. The markers examined allowed us to distinguish populations from large-scale, intensive farming region (cotton belts) versus populations from areas of small-scale farming

  7. Coffee has hepatoprotective benefits in Brazilian patients with chronic hepatitis C even in lower daily consumption than in American and European populations

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    Silmara Rodrigues Machado

    2014-04-01

    Full Text Available The potential role of coffee as a hepatoprotective substance for chronic liver diseases has been widely discussed. Our main aim was to evaluate the effect of coffee intake regarding clinical, biochemical tests and liver biopsy data in treatment naïve patients with chronic hepatitis C. One hundred and thirty-six patients with chronic hepatitis C, diagnosed through liver biopsy, or by means of clinical, ultrasound or endoscopic signs of cirrhosis, were assessed by determination of biochemical tests, metabolic and morphological alterations. Food frequency was scrutinized by using a structured questionnaire. Coffee intake represented more than 90% of the total daily caffeine, and the 75th percentile was 4-Brazilian coffee-cup/day (>255mL/day or >123mg caffeine/day. According to caffeine intake, patients were divided into two groups (123mg caffeine/day. Patients with higher ingestion of caffeine had lower serum levels of aspartate aminotransferase (× upper limit of normal (1.8±1.5 vs 2.3±1.5, p=0.04, lower frequencies of advanced (F3, F4 fibrosis (23.5% vs 54.5%, p<0.001 and of histological activity grade (A3, A4 observed in liver biopsies (13.8% vs 36.9%, p<0.001. By multivariate logistic regression, fibrosis was independently associated with caffeine intake (OR- 0.16; 95%CI - 0.03-0.80; p=0.026, γ-glutamil transferase serum levels and morphological activity. But only fibrosis was associated with histological activity. In conclusion caffeine consumption greater than 123mg/day was associated with reduced hepatic fibrosis. In addition, this study supports the assumption that coffee intake has hepatoprotective benefits for Brazilian patients with chronic hepatitis C, even in lower doses than that of American and European population intake.

  8. Clinical evaluation of nonsyndromic dental anomalies in Dravidian population: A cluster sample analysis

    OpenAIRE

    Yamunadevi, Andamuthu; Selvamani, M.; Vinitha, V.; Srivandhana, R.; Balakrithiga, M.; Prabhu, S; Ganapathy, N

    2015-01-01

    Aim: To record the prevalence rate of dental anomalies in Dravidian population and analyze the percentage of individual anomalies in the population. Methodology: A cluster sample analysis was done, where 244 subjects studying in a dental institution were all included and analyzed for occurrence of dental anomalies by clinical examination, excluding third molars from analysis. Results: 31.55% of the study subjects had dental anomalies and shape anomalies were more prevalent (22.1%), followed b...

  9. Childhood Trauma in Substance Use Disorder and Depression: An Analysis by Gender among a Brazilian Clinical Sample

    Science.gov (United States)

    Tucci, Adriana M.; Kerr-Correa, Florence; Souza-Formigoni, Maria Lucia O.

    2010-01-01

    Objective: In this study, we compared the frequency and intensity of childhood traumas in alcohol- or other drug-dependent patients, in patients with depression, and in a control group without psychiatric diagnoses. Methods: The study had a retrospective design of a clinical sample of men and women from the groups listed above. They were evaluated…

  10. Bauer-7-en-3{beta}-yl acetate: a major constituent of unusual samples of Brazilian propolis

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Erica Weinstein [Secretaria da Agricultura e Abastecimento, Pindamonhangaba, SP (Brazil). Agencia Paulista de Tecnologia dos Agronegocios; Message, Dejair [Vicosa Univ., MG (Brazil). Inst. de Biociencias. Dept. de Biologia Animal; Negri, Giuseppina; Salatino, Antonio [Sao Paulo Univ., SP (Brazil). Dept. de Botanica

    2006-03-15

    The pentacyclic triterpenoid bauer-7-en-3{beta}-yl acetate was obtained from thloroform extract of an unusual sample of propolis from southeast Brazil with the yield of 7%. The compound was identified by comparison of IR, MS and NMR analysis with published data. (author)

  11. Tritium determination in aqueous samples by using LSC Quantulus in CDTN - Brazilian Development Center for Nuclear Technology, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Temba, Eliane S.C.; Moreira, Rubens M. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Meio Ambiente e Tecnicas Nucleares]. E-mails: esct@cdtn.br; rubens@cdtn.br; Mingote, Raquel M. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Servico de Quimica e Radioquimica]. E-mail: mingote@cdtn.br

    2007-07-01

    Tritium is the radioisotope of hydrogen which disintegrates by emission of beta particle to {sup 3}He. Its determination is important in several applications such as in oil recovery, where tritiated tracers are added to injection fluids, or in groundwater surveys, to estimate the recharging of aquifers, among others applications. Liquid scintillation spectrometry is an appropriate method for determining low-level contents of tritium in aqueous samples. We used a low background liquid scintillation system detector, Perkin Elmer - Wallac QuantulusTM 1220, recently acquired by Nuclear Technology Development Center (CDTN). The purpose of this work is the optimization of the counting performance for tritium determination in water samples by direct counting. For definition of optimum parameters we compare two cocktails, Instagel Plus{sup TM} and Optiphase Hisafe{sup TM} 3. The ratios of sample/cocktail studied were 8/10, 10/10 and 10/12. The stability of the sample/cocktail mixture was also analysed by the loss of mass and by the luminescence spectrum. The counting conditions were 60 minutes counts for each vial, in the selected counting windows 50-200 and 1-200 channels. The vial used was 20 mL polyethylene. For background estimation we used a very old water (dead water) from Thermas Antonio Carlos, Pocos de Caldas, Brazil. The good results obtained in national and international laboratory intercomparison programs indicate the excellence of the tritium analysis at CDTN. (author)

  12. Early detection of nonnative alleles in fish populations: When sample size actually matters

    Science.gov (United States)

    Croce, Patrick Della; Poole, Geoffrey C.; Payne, Robert A.; Gresswell, Bob

    2017-01-01

    Reliable detection of nonnative alleles is crucial for the conservation of sensitive native fish populations at risk of introgression. Typically, nonnative alleles in a population are detected through the analysis of genetic markers in a sample of individuals. Here we show that common assumptions associated with such analyses yield substantial overestimates of the likelihood of detecting nonnative alleles. We present a revised equation to estimate the likelihood of detecting nonnative alleles in a population with a given level of admixture. The new equation incorporates the effects of the genotypic structure of the sampled population and shows that conventional methods overestimate the likelihood of detection, especially when nonnative or F-1 hybrid individuals are present. Under such circumstances—which are typical of early stages of introgression and therefore most important for conservation efforts—our results show that improved detection of nonnative alleles arises primarily from increasing the number of individuals sampled rather than increasing the number of genetic markers analyzed. Using the revised equation, we describe a new approach to determining the number of individuals to sample and the number of diagnostic markers to analyze when attempting to monitor the arrival of nonnative alleles in native populations.

  13. Brazilian propolis extract used as an additive to decrease methane emissions from the rumen microbial population in vitro.

    Science.gov (United States)

    Santos, Nadine Woruby; Zeoula, Lucia Maria; Yoshimura, Emerson Henri; Machado, Erica; Macheboeuf, Didier; Cornu, Agnès

    2016-06-01

    Propolis is a product that is rich in phenolic compounds and can be utilized in animal nutrition as a dietary additive. In this study, the effects of a Brazilian green propolis extract on rumen fermentation and gas production were determined. The fate of propolis phenolic compounds in the rumen medium was also investigated. Fermentation was done in 24-h batches over three periods. Inoculates were obtained from cows fed on grassland hay and concentrate. Propolis extract in a hydroalcoholic solution was applied at increasing doses to the substrate (1 to 56 g/kg). The fermentation substrate consisted on a mixture of alfalfa hay, soybean meal, and wheat grain mixture in dry matter. After 24 h of fermentation, seven new compounds were observed in the medium in amounts that correlated to the propolis dose. The dose of propolis extract linearly decreased the pH of the medium and linearly increased propionate production, which reduced the acetate-to-propionate ratio and influenced the total production of short-chain fatty acids. Propolis also linearly reduced methane production and increased the carbon dioxide-to-methane ratio. Ammonia nitrogen levels and in vitro digestibility of organic matter were similar among the treatments. The combination of increased propionate production and decreased methane production suggests better energy utilization from the feed.

  14. Fatores associados ao padrão de consumo alimentar da população brasileira urbana Factors associated with dietary patterns in the urban Brazilian population

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    Rosely Sichieri

    2003-01-01

    Full Text Available Avaliou-se os fatores que explicariam diferentes padrões de consumo alimentar da população urbana brasileira, com base na Pesquisa sobre Padrões de Vida, realizada no Nordeste e no Sudeste em 1996-1997. Foram incluídos os dados de consumo familiar (últimos 14 dias a partir de uma listagem com 28 alimentos de 5.121 adultos na faixa etária de 20 a 50 anos, após exclusão das gestantes, das lactantes e das pessoas que referiram problema de saúde crônico. Os padrões de consumo alimentar foram definidos pela análise de componentes principais, onde o valor de carga de uma determinada variável (alimento é proporcional à sua contribuição para o fator (padrão de consumo em questão, com a carga negativa indicando que a variável está inversamente associada com o fator, enquanto a positiva indica uma associação direta. A Região Nordeste comparada à Região Sudeste associou-se negativamente a um padrão misto, quando todos os alimentos foram igualmente importantes. O índice de massa corporal associou-se positivamente ao padrão misto, e praticar atividade física e ser negro associou-se negativamente a este padrão. Escolaridade e renda foram as variáveis que mais explicaram o consumo alimentar, mas mesmo ajustando por elas, a região de residência foi o terceiro maior componente explicativo.This study evaluated factors associated with dietary patterns in the Brazilian population based on the Living Standards Survey conducted in the Northeast and Southeast regions of the country. Multi-stage probability sampling was employed to select the households, and for the present analysis 5,121 adults aged 20 to 50 years were included. Pregnant women and individuals reporting chronic health conditions were excluded. Through principal component analysis, two major family dietary patterns were identified: a mixed pattern, in which all groups and foods have approximately the same factor loading, and a second pattern based main1y on rice and

  15. Genética comunitária e hemofilia em uma população brasileira Community genetics and hemophilia in a Brazilian population

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    Vânia Maria Caio

    2001-06-01

    Full Text Available A hemofilia é uma doença hemorrágica importante no Brasil, afetando cerca de um em cada dez mil indivíduos do sexo masculino. A autopercepção do portador a respeito da hemofilia e a sua interação com a comunidade são relevantes na abordagem clínica dessa doença. Nós investigamos vários aspectos sociais, psicológicos e comunitários da hemofilia em uma população brasileira (Campinas, São Paulo. Entrevistamos trinta portadores adultos da hemofilia, uma amostra-controle composta por 73 de seus irmãos normais do sexo masculino e 641 indivíduos da comunidade. A integração comunitária dos portadores da hemofilia mostrou-se afetada apenas nos aspectos sócio-econômicos, sem alteração no que diz respeito ao casamento, à procriação e à aquisição de melhores níveis educacionais. Observaram-se entre os portadores da hemofilia altos níveis de auto-rotulação, acompanhados de depressão, ansiedade e insegurança. A comunidade apresentou uma alta freqüência de completo desconhecimento a respeito da hemofilia (49%, demonstrando contra os hemofílicos os preconceitos normalmente observados contra os portadores de doenças contagiosas, como a AIDS. O trabalho sugere a estruturação de programa comunitário, visando à melhor adequação psicossocial dos portadores da hemofilia.Hemophilia is an important hemorrhagic disease in Brazil, affecting about 1 out of every 10,000 males. Patient's self-perception of hemophilia and interaction with the community are relevant to the clinical management of this disease. We investigated several social, psychological, and community aspects of hemophilia in a Brazilian population (Campinas, São Paulo State, interviewing 30 hemophiliac males, a control sample comprised of 73 non-hemophiliac brothers, and 641 individuals from the community. According to our results, more severe social disability in the hemophiliac patient was related to economic factors, mainly unemployment; however, no

  16. Transgender Population Size in the United States: a Meta-Regression of Population-Based Probability Samples

    Science.gov (United States)

    Sevelius, Jae M.

    2017-01-01

    Background. Transgender individuals have a gender identity that differs from the sex they were assigned at birth. The population size of transgender individuals in the United States is not well-known, in part because official records, including the US Census, do not include data on gender identity. Population surveys today more often collect transgender-inclusive gender-identity data, and secular trends in culture and the media have created a somewhat more favorable environment for transgender people. Objectives. To estimate the current population size of transgender individuals in the United States and evaluate any trend over time. Search methods. In June and July 2016, we searched PubMed, Cumulative Index to Nursing and Allied Health Literature, and Web of Science for national surveys, as well as “gray” literature, through an Internet search. We limited the search to 2006 through 2016. Selection criteria. We selected population-based surveys that used probability sampling and included self-reported transgender-identity data. Data collection and analysis. We used random-effects meta-analysis to pool eligible surveys and used meta-regression to address our hypothesis that the transgender population size estimate would increase over time. We used subsample and leave-one-out analysis to assess for bias. Main results. Our meta-regression model, based on 12 surveys covering 2007 to 2015, explained 62.5% of model heterogeneity, with a significant effect for each unit increase in survey year (F = 17.122; df = 1,10; b = 0.026%; P = .002). Extrapolating these results to 2016 suggested a current US population size of 390 adults per 100 000, or almost 1 million adults nationally. This estimate may be more indicative for younger adults, who represented more than 50% of the respondents in our analysis. Authors’ conclusions. Future national surveys are likely to observe higher numbers of transgender people. The large variety in questions used to ask

  17. Inspecting close maternal relatedness: Towards better mtDNA population samples in forensic databases

    Science.gov (United States)

    Bodner, Martin; Irwin, Jodi A.; Coble, Michael D.; Parson, Walther

    2011-01-01

    Reliable data are crucial for all research fields applying mitochondrial DNA (mtDNA) as a genetic marker. Quality control measures have been introduced to ensure the highest standards in sequence data generation, validation and a posteriori inspection. A phylogenetic alignment strategy has been widely accepted as a prerequisite for data comparability and database searches, for forensic applications, for reconstructions of human migrations and for correct interpretation of mtDNA mutations in medical genetics. There is continuing effort to enhance the number of worldwide population samples in order to contribute to a better understanding of human mtDNA variation. This has often lead to the analysis of convenience samples collected for other purposes, which might not meet the quality requirement of random sampling for mtDNA data sets. Here, we introduce an additional quality control means that deals with one aspect of this limitation: by combining autosomal short tandem repeat (STR) marker with mtDNA information, it helps to avoid the bias introduced by related individuals included in the same (small) sample. By STR analysis of individuals sharing their mitochondrial haplotype, pedigree construction and subsequent software-assisted calculation of likelihood ratios based on the allele frequencies found in the population, closely maternally related individuals can be identified and excluded. We also discuss scenarios that allow related individuals in the same set. An ideal population sample would be representative for its population: this new approach represents another contribution towards this goal. PMID:21067986

  18. On the Estimation of Heritability with Family-Based and Population-Based Samples

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    Youngdoe Kim

    2015-01-01

    Full Text Available For a family-based sample, the phenotypic variance-covariance matrix can be parameterized to include the variance of a polygenic effect that has then been estimated using a variance component analysis. However, with the advent of large-scale genomic data, the genetic relationship matrix (GRM can be estimated and can be utilized to parameterize the variance of a polygenic effect for population-based samples. Therefore narrow sense heritability, which is both population and trait specific, can be estimated with both population- and family-based samples. In this study we estimate heritability from both family-based and population-based samples, collected in Korea, and the heritability estimates from the pooled samples were, for height, 0.60; body mass index (BMI, 0.32; log-transformed triglycerides (log TG, 0.24; total cholesterol (TCHL, 0.30; high-density lipoprotein (HDL, 0.38; low-density lipoprotein (LDL, 0.29; systolic blood pressure (SBP, 0.23; and diastolic blood pressure (DBP, 0.24. Furthermore, we found differences in how heritability is estimated—in particular the amount of variance attributable to common environment in twins can be substantial—which indicates heritability estimates should be interpreted with caution.

  19. Frequency of polymorphisms of genes coding for HIV-1 co-receptors CCR5 and CCR2 in a Brazilian population

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    Munerato Patrícia

    2003-01-01

    Full Text Available Entry of human immunodeficiency type 1 virus (HIV-1 into target cells requires both CD4and one of the chemokine receptors. Viruses predominantly use one, or occasionally both, of the major co-receptors CCR5 and CXCR4, although other receptors, including CCR2B and CCR3, function as minor co-receptors. A 32-nucleotide deletion (delta32 within the beta-chemokine receptor 5 gene (CCR5 has been described in subjects who remain uninfected despite extensive exposition to HIV-1. The heterozygous genotype delays disease progression. This allele is common among Caucasians, but has not been found in people of African or Asian ancestry. A more common transition involving a valine to isoleucine switch in transmembrane domain I of CCR2B (64I, with unknown functional consequences, was found to delay disease progression but not to reduce infection risk. As the Brazilian population consists of a mixture of several ethnic groups, we decided to examine the genotype frequency of these polymorphisms in this country. There were 11.5% CCR5 heterozygotes among the HIV-1 infected population and 12.5% among uninfected individuals, similar to data from North America and Western Europe. The prevalence of CCR2-64I homozygotes and heterozygotes was 0.06 and 15.2%, respectively, also similar to what is known for North America and Western Europe.

  20. Dental approach in the pediatric oncology patient: characteristics of the population treated at the dentistry unit in a pediatric oncology brazilian teaching hospital

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    Camila Carrillo

    2010-01-01

    Full Text Available OBJECTIVES: The objective of this paper was to characterize the population seen at the dentistry unit of the hematology-oncology service of the Oncology-Hematology Service, Instituto da Criança at the Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo. Oral problems resulting from cancer therapy increase the risk of infection, length of hospital stay, treatment cost and negative impact on the course and prognosis of the disease. METHOD: Of the 367 medical records of cancer patients seen from November 2007 until December 2008: 186 with a cancer diagnosis and complete clinical data were selected, while 20 with a cancer diagnosis and incomplete records were excluded; 161 medical records with only hematological diagnosis were also excluded. The following characteristics were assessed: ethnicity, gender, age, diagnosis and characteristics of the neoplasm, cancer therapy status and performed dental procedures. RESULTS: Review of 1,236 visits indicated that 54% (n=100 of the patients had blood cancers, 46% (n=86 had solid tumors and 63% were undergoing anticancer therapy. The proportion of males (52.7% in the study population was slightly greater. The most common cancer was acute lymphocytic leukemia (32.2%. Cancer occurred more often among those patients aged 5 to 9 years. The most common dental procedures were restorative treatment, preventive treatment and removal of infectious foci. CONCLUSION: The characteristics of the studied population were similar to those of the general Brazilian and global populations, especially regarding gender and diagnosis distributions. The aim of implementation of the dentistry unit was to maintain good oral health and patients' quality of life, which is critical to provide oral care and prevent future oral problems.

  1. Variables associated with health-related quality of life in a Brazilian sample of patients from a tertiary outpatient clinic for depression and anxiety disorders

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    Bianca Schwab

    2015-12-01

    Full Text Available Introduction: Health-related quality of life (HRQOL assessment tools have been broadly used in the medical context. These tools are used to measure the subjective impact of the disease on patients. The objective of this study was to evaluate the variables associated with HRQOL in a Brazilian sample of patients followed up in a tertiary outpatient clinic for depression and anxiety disorders. Method: Cross-sectional study. Independent variables were those included in a sociodemographic questionnaire and the Hospital Anxiety and Depression Scale (HADS scores. Dependent variables were those included in the short version of the World Health Organization Quality of Life (WHOQOL-BREF and the scores for its subdomains (overall quality of life and general health, physical health, psychological health, social relationships, and environment. A multiple linear regression analysis was used to find the variables independently associated with each outcome. Results: Seventy-five adult patients were evaluated. After multiple linear regression analysis, the HADS scores were associated with all outcomes, except social relationships (p = 0.08. Female gender was associated with poor total scores, as well as psychological health and environment. Unemployment was associated with poor physical health. Conclusion: Identifying the factors associated with HRQOL and recognizing that depression and anxiety are major factors are essential to improve the care of patients.

  2. -765 G>C POLYMORPHISM OF THE COX-2 GENE AND GASTRIC CANCER RISK IN BRAZILIAN POPULATION

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    Vanessa Maria de Lima Pazine CAMPANHOLO

    2014-04-01

    Full Text Available Context Genomic alterations play important roles in gastric cancer carcinogenesis. Cyclooxygenases (COX are important enzymes in the maintenance of mucosal integrity and in pathological processes, mainly in inflammation and cancer. The -765G>C COX-2 polymorphism has been implicated in gastric cancer risk. Objectives To evaluate the COX-2 gene polymorphism as a predictor of gastric cancer risk. Methods One hundred gastric cancer patients and 150 controls were enrolled from a Brazilian centre. Personal data regarding related risk factors, including alcohol consumption and smoking behavior, were collected via questionnaire. DNA was extracted from peripheral blood and the genotypes were analyzed using PCR-restriction fragment length polymorphism. Results G/G, G/C and C/C genotypes frequencies was 42.7%, 50% and 7.3%, respectively in controls and 59.0%, 34.0% and 7.0% in gastric cancer. The frequency of the genotypes differed between the groups (P = 0.033. A higher risk of gastric cancer was associated with COX-2 -765G/G genotype (P = 0.048; OR:1.98, 95% CI = 1.01-3.90. Alcohol consumption and smoking in patients with -765G/G genotype also increased the risk of gastric cancer. Conclusions The -765G/G genotype and the -765G allele had been associated with an increased risk for gastric cancer. The presence of smoking and alcohol consumption increased the risk for gastric cancer in subjects with -765G/G genotype compared with the control group. Polymorphism of COX-2 gene and gastric cancer risk.

  3. The Association of the Immune Response Genes to Human Papillomavirus-Related Cervical Disease in a Brazilian Population

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    Amanda Vansan Marangon

    2013-01-01

    Full Text Available The genetic variability of the host contributes to the risk of human papillomavirus (HPV-related cervical disease. Immune response genes to HPV must be investigated to define patients with the highest risk of developing malignant disease. The aim of this study was to investigate the association of polymorphic immune response genes, namely KIR, HLA class I and II, and single-nucleotide polymorphisms (SNPs of cytokines with HPV-related cervical disease. We selected 79 non-related, admixed Brazilian women from the state of Paraná, southern region of Brazil, who were infected with high carcinogenic risk HPV and present cervical intraepithelial neoplasia grade 3 (CIN3, and 150 HPV-negative women from the same region matched for ethnicity. KIR genes were genotyped using an in-house PCR-SSP. HLA alleles were typed using a reverse sequence-specific oligonucleotide technique. SNPs of TNF −308G>A, IL6 −174G>C, IFNG +874T>A, TGFB1 +869T>C +915G>C, and IL10 −592C>A −819C>T −1082G>A were evaluated using PCR-SSP. The KIR genes were not associated with HPV, although some pairs of i(inhibitoryKIR-ligands occurred more frequently in patients, supporting a role for NK in detrimental chronic inflammatory and carcinogenesis. Some HLA haplotypes were associated with HPV. The associations of INFG and IL10 SNPs potentially reflect impaired or invalid responses in advanced lesions.

  4. Eating patterns in a population-based sample of children aged 5 to 7 years

    DEFF Research Database (Denmark)

    Micali, Nadia; Simonoff, Emily; Elberling, Hanne

    2011-01-01

    There is still a lack of research on childhood eating patterns and their correlates in relation to psychopathology and parentally perceived impact in general population samples. We aimed to determine which eating patterns were more likely to be identified as problematic by parents, and their impa...

  5. Autosomal STR allele frequencies for the CODIS system from a large random population sample in Chile.

    Science.gov (United States)

    Vergara, Ismael A; Villouta, Pamela; Herrera, Sandra; Melo, Francisco

    2012-05-01

    The thirteen autosomal STR loci of the CODIS system were typed from DNA of 732 unrelated male individuals sampled from different locations in Chile. This is the first report of allele frequencies for the thirteen STRs loci defined in the CODIS system from the Chilean population.

  6. Results for five sets of forensic genetic markers studied in a Greek population sample

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Skitsa, I; Steinmeier, E;

    2015-01-01

    A population sample of 223 Greek individuals was typed for five sets of forensic genetic markers with the kits NGM SElect™, SNPforID 49plex, DIPplex(®), Argus X-12 and PowerPlex(®) Y23. No significant deviation from Hardy-Weinberg expectations was observed for any of the studied markers after Hol...

  7. Population and Family Education. Draft Sample Instructional Materials. Science/Mathematics.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    The sample first-draft materials, produced by participants at a UNESCO regional workshop on population and family life, are designed as a reference tool to be used by curriculum developers. Divided into two major parts -- in biological science and in mathematics -- the teaching guide is for secondary level students. The first part, consisting of…

  8. Population Education in Social Studies: Some Sample Lessons for the Secondary Level.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and the Pacific.

    This booklet consists of 10 sample lessons integrating population education into the social studies. It is one of four in a series. Materials differ from those in an earlier series (1980) in that lessons are presented at the secondary level only; there is no duplication of lessons from the earlier series in terms of content and teaching…

  9. Population Education in Mathematics: Some Sample Lessons for the Secondary Level.

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and the Pacific.

    This booklet consists of five sample lessons integrating population education into mathematics instruction. It is one of four in a series. Materials differ from those in an earlier series (1980) in that lessons are presented at the secondary level only; there is no duplication of lessons from the earlier series in content and teaching strategies.…

  10. Efficient Importance Sampling Heuristics for the Simulation of Population Overflow in Feed-Forward Queueing Networks

    NARCIS (Netherlands)

    Nicola, Victor F.; Zaburnenko, Tatiana S.

    2006-01-01

    In this paper we propose a state-dependent importance sampling heuristic to estimate the probability of population overflow in feed-forward networks. This heuristic attempts to approximate the “optimal” state-dependent change of measure without the need for difficult analysis or costly optimization i

  11. Importance Sampling Simulation of Population Overflow in Two-node Tandem Networks

    NARCIS (Netherlands)

    Nicola, Victor F.; Zaburnenko, Tatiana S.; Baier, C.; Chiola, G.; Smirni, E.

    2005-01-01

    In this paper we consider the application of importance sampling in simulations of Markovian tandem networks in order to estimate the probability of rare events, such as network population overflow. We propose a heuristic methodology to obtain a good approximation to the 'optimal' state-dependent ch

  12. Gaining Access to Economically Marginalized Rural Populations: Lessons Learned from Nonprobability Sampling

    Science.gov (United States)

    Mammen, Sheila; Sano, Yoshie

    2012-01-01

    Poverty is a significant problem in rural America. Gaining access to economically marginalized rural populations in order to recruit individuals to participate in a research study, however, is often a challenge. This article compares three different nonprobability sampling techniques that have been used to recruit rural, low-income…

  13. Psychological Abuse between Parents: Associations with Child Maltreatment from a Population-Based Sample

    Science.gov (United States)

    Chang, Jen Jen; Theodore, Adrea D.; Martin, Sandra L.; Runyan, Desmond K.

    2008-01-01

    Objective: This study examined the association between partner psychological abuse and child maltreatment perpetration. Methods: This cross-sectional study examined a population-based sample of mothers with children aged 0-17 years in North and South Carolina (n = 1,149). Mothers were asked about the occurrence of potentially neglectful or abusive…

  14. Differentiating quiescent cancer cell populations in heterogeneous samples with fluorescence lifetime imaging

    Science.gov (United States)

    Heaster, Tiffany M.; Walsh, Alex J.; Skala, Melissa C.

    2016-03-01

    Measurement of relative fluorescence intensities of NAD(P)H and FAD with fluorescence lifetime imaging (FLIM) allows metabolic characterization of cancerous populations and correlation to treatment response. However, quiescent populations of cancer cells introduce heterogeneity to the tumor and exhibit resistance to standard therapies, requiring a better understanding of this influence on treatment outcome. Significant differences were observed between proliferating and quiescent cell populations upon comparison of respective redox ratios (pFAD lifetimes (p<0.05) across monolayers and in mixed samples. These results demonstrate that metabolic activity may function as a marker for separation and characterization of proliferating and quiescent cancer cells within mixed samples, contributing to comprehensive investigation of heterogeneity-dependent drug resistance.

  15. screening for cognitive impairment in late onset depression in a Brazilian sample using the BBRC-edu

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    Tânia Maria da Silva Novaretti

    Full Text Available ABSTRACT Depression and dementia are the most prevalent neuropsychiatric disorders in the elderly population. Alzheimer's disease is the leading cause of dementia in most countries, being responsible for more than half of all dementia cases. Late-onset depression is a frequent cause of cognitive decline in the elderly. Differentiating between cognitive impairment secondary to depression and incipient dementia poses a challenge in the clinical setting. Objective: To evaluate the performance of elderly depressed patients using the BBRC-Edu. Methods: We studied 25 patients with late onset depression (mean age: 73.6 y (6.6; schooling: 9.1 y (5.7 and 30 patients with mild AD (mean age 76.6 y (5.4; schooling: 7.5 y (7.1, who were compared to a control group of 30 healthy elderly (mean age 73.8 y (5.8; schooling: 9.1 y (5.4 using the CERAD and BBRC-Edu batteries. Results: For the CERAD battery, depressed patients performed better than AD patients on all tasks (p0.05, and performed poorer than controls on verbal fluency (animals and Word List Recall tasks (p0.05, and performed worse than controls on Learning (second presentation and verbal fluency (fruits tasks (p<0.0001. Conclusion: Overall performance on the BBRC-Edu allowed differentiation of controls and depressed patients from AD patients.

  16. Results for five sets of forensic genetic markers studied in a Greek population sample.

    Science.gov (United States)

    Tomas, C; Skitsa, I; Steinmeier, E; Poulsen, L; Ampati, A; Børsting, C; Morling, N

    2015-05-01

    A population sample of 223 Greek individuals was typed for five sets of forensic genetic markers with the kits NGM SElect™, SNPforID 49plex, DIPplex®, Argus X-12 and PowerPlex® Y23. No significant deviation from Hardy-Weinberg expectations was observed for any of the studied markers after Holm-Šidák correction. Statistically significant (Pstudied X-chromosome linkage groups. AMOVA analyses of the five sets of markers did not show population structure when the individuals were grouped according to their geographic origin. The Greek population grouped closely to the other European populations measured by F(ST)(*) distances. The match probability ranged from a value of 1 in 2×10(7) males by using haplotype frequencies of four X-chromosome haplogroups in males to 1 in 1.73×10(21) individuals for 16 autosomal STRs.

  17. Phosphatase Activity of Microbial Populations in Different Milk Samples in Relation to Protein and Carbohydrate Content

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    Sosanka Protim SANDILYA

    2014-12-01

    Full Text Available Cattle milk is a rich source of protein, carbohydrate, vitamins, minerals and all other major and micro nutrients. At a moderate pH, milk is an excellent media for the growth of microbes and thus, intake of raw milk is precarious. In this study, attempt was made for a qualitative study of eight raw milk samples of different varieties of cow and goat milk, collected from Jorhat district of Assam, India, on the basis of nutritional value and microbial population. The highest microbial population was found in the milk collected from cross hybrid variety of cow, whereas microbial contamination was the least in Jersey cow milk. Samples of C1 (Jersey cow variety showed presence of the highest amount of protein and carbohydrate content as compared to the others. Almost all the milk samples showed positive acid and alkaline phosphatase activity. Maximum acid phosphatase activity was observed in cross hybrid cow milk, whereas local cow milk exhibited the highest alkaline phosphatase activity. Phosphatase activity did not show any co-relationship with microbial population of the milk samples. Similarly, the protein and carbohydrate content of the samples did not have any significant impact on both acid and alkaline phosphatase activity.

  18. Sampling Nomads: A New Technique for Remote, Hard-to-Reach, and Mobile Populations

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    Himelein Kristen

    2014-06-01

    Full Text Available Livestock are an important component of rural livelihoods in developing countries, but data about this source of income and wealth are difficult to collect due to the nomadic and seminomadic nature of many pastoralist populations. Most household surveys exclude those without permanent dwellings, leading to undercoverage. In this study, we explore the use of a random geographic cluster sample (RGCS as an alternative to the household-based sample. In this design, points are randomly selected and all eligible respondents found inside circles drawn around the selected points are interviewed. This approach should eliminate undercoverage of mobile populations. We present results of an RGCS survey with a total sample size of 784 households to measure livestock ownership in the Afar region of Ethiopia in 2012. We explore the RGCS data quality relative to a recent household survey, and discuss the implementation challenges.

  19. Photographic capture-recapture sampling for assessing populations of the Indian gliding lizard Draco dussumieri.

    Science.gov (United States)

    Sreekar, Rachakonda; Purushotham, Chetana B; Saini, Katya; Rao, Shyam N; Pelletier, Simon; Chaplod, Saniya

    2013-01-01

    The usage of invasive tagging methods to assess lizard populations has often been criticised, due to the potential negative effects of marking, which possibly cause increased mortality or altered behaviour. The development of safe, less invasive techniques is essential for improved ecological study and conservation of lizard populations. In this study, we describe a photographic capture-recapture (CR) technique for estimating Draco dussumieri (Agamidae) populations. We used photographs of the ventral surface of the patagium to identify individuals. To establish that the naturally occurring blotches remained constant through time, we compared capture and recapture photographs of 45 pen-marked individuals after a 30 day interval. No changes in blotches were observed and individual lizards could be identified with 100% accuracy. The population density of D. dussumieri in a two hectare areca-nut plantation was estimated using the CR technique with ten sampling occasions over a ten day period. The resulting recapture histories for 24 individuals were analysed using population models in the program CAPTURE. All models indicated that nearly all individuals were captured. The estimated probability for capturing D. dussumieri on at least one occasion was 0.92 and the estimated population density was 13±1.65 lizards/ha. Our results demonstrate the potential for applying CR to population studies in gliding lizards (Draco spp.) and other species with distinctive markings.

  20. Photographic capture-recapture sampling for assessing populations of the Indian gliding lizard Draco dussumieri.

    Directory of Open Access Journals (Sweden)

    Rachakonda Sreekar

    Full Text Available The usage of invasive tagging methods to assess lizard populations has often been criticised, due to the potential negative effects of marking, which possibly cause increased mortality or altered behaviour. The development of safe, less invasive techniques is essential for improved ecological study and conservation of lizard populations. In this study, we describe a photographic capture-recapture (CR technique for estimating Draco dussumieri (Agamidae populations. We used photographs of the ventral surface of the patagium to identify individuals. To establish that the naturally occurring blotches remained constant through time, we compared capture and recapture photographs of 45 pen-marked individuals after a 30 day interval. No changes in blotches were observed and individual lizards could be identified with 100% accuracy. The population density of D. dussumieri in a two hectare areca-nut plantation was estimated using the CR technique with ten sampling occasions over a ten day period. The resulting recapture histories for 24 individuals were analysed using population models in the program CAPTURE. All models indicated that nearly all individuals were captured. The estimated probability for capturing D. dussumieri on at least one occasion was 0.92 and the estimated population density was 13±1.65 lizards/ha. Our results demonstrate the potential for applying CR to population studies in gliding lizards (Draco spp. and other species with distinctive markings.

  1. Evaluation of Rhipicephalus (Boophilus microplus (Acari: Ixodidae resistance to different acaricide formulations using samples from Brazilian properties

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    Leandro de Oliveira Souza Higa

    2016-01-01

    Full Text Available Abstract The Rhipicephalus (Boophilus microplus tick is responsible for considerable economic losses in Brazil, causing leather damage, weight loss and reduced milk production in cattle and results in the transmission of pathogens. Currently, the main method for controlling this tick is using acaricides, but their indiscriminate use is one of the major causes of resistance dissemination. In this study, the adult immersion test (AIT was used to evaluate resistance in ticks from 28 properties located in five different states (Mato Grosso do Sul, Mato Grosso, Ceará, São Paulo, e Minas Gerais and the Distrito Federal (DF of Brazil. The resistance was found in 47.64% of the repetitions demonstrating an efficacy of less than 90% in various locations throughout the country. The larvae packet test was used to evaluate samples from ten properties in four states (Mato Grosso do Sul, Mato Grosso, Minas Gerais and Espírito Santo. Spray products belonging to the main classes of acaricides, including combination formulations, were used in both types of test. New cases of resistance were found on properties within the states of Ceará, Espírito Santo and Mato Grosso, where such resistance was not previously reported.

  2. Zipf Law for Brazilian Cities

    CERN Document Server

    Moura, N J; Jr., Newton J. Moura; Ribeiro, Marcelo B.

    2006-01-01

    This work studies the Zipf Law for cities in Brazil. Data from censuses of 1970, 1980, 1991 and 2000 were used to select a sample containing only cities with 30,000 inhabitants or more. The results show that the population distribution in Brazilian cities does follow a power law similar to the ones found in other countries. Estimates of the power law exponent were found to be 2.22 +/- 0.34 for the 1970 and 1980 censuses, and 2.26 +/- 0.11 for censuses of 1991 and 2000. More accurate results were obtained with the maximum likelihood estimator, showing an exponent equal to 2.41 for 1970 and 2.36 for the other three years.

  3. Effects of liming and legume/cereal cropping on populations of indigenous rhizobia in an acid Brazilian Oxisol

    NARCIS (Netherlands)

    Andrade, D.S.; Murphy, P.J.; Giller, K.E.

    2002-01-01

    Given the acid soil conditions in many regions of common bean production in the tropics and the deleterious effects of soil acidity on rhizobia, studies to assess survival of Phaseolus-nodulating rhizobial populations in acidic soils are important to ensure establishment of effective N2-fixing symbi

  4. Sample-size calculations for multi-group comparison in population pharmacokinetic experiments.

    Science.gov (United States)

    Ogungbenro, Kayode; Aarons, Leon

    2010-01-01

    This paper describes an approach for calculating sample size for population pharmacokinetic experiments that involve hypothesis testing based on multi-group comparison detecting the difference in parameters between groups under mixed-effects modelling. This approach extends what has been described for generalized linear models and nonlinear population pharmacokinetic models that involve only binary covariates to more complex nonlinear population pharmacokinetic models. The structural nonlinear model is linearized around the random effects to obtain the marginal model and the hypothesis testing involving model parameters is based on Wald's test. This approach provides an efficient and fast method for calculating sample size for hypothesis testing in population pharmacokinetic models. The approach can also handle different design problems such as unequal allocation of subjects to groups and unbalanced sampling times between and within groups. The results obtained following application to a one compartment intravenous bolus dose model that involved three different hypotheses under different scenarios showed good agreement between the power obtained from NONMEM simulations and nominal power.

  5. Impact of forest fragment size on the population structure of three palm species (Arecaceae) in the Brazilian Atlantic rainforest.

    Science.gov (United States)

    Portela, Rita de Cássia Quitete; dos Santos, Flavio Antonio Maes

    2014-06-01

    The main threats to natural populations in terrestrial ecosystems have been widly recognized to be the habitat fragmentation and the exploitation of forest products. In this study, we compared the density of the populations and the structure of three tropical palm species, Astrocaryum aculeatissimum, Euterpe edulis and Geonoma schottiana. For this, we selected five forest fragments of different sizes (3 500ha, 2 400ha, 57ha, 21ha and 19ha) where palms were censused in nine 30 x 30m plots. We tracked the palms survival from 2005 to 2007, and recorded all new individuals encountered. Each individual was assigned in one of the five ontogenetic stages: seedling, infant, juvenile, immature and reproductive. The demographic structure of each palm species was analyzed and compared by a generalized linear model (GLM). The analysis was performed per palm species. The forest fragment area and the year of observation were explanatory variables, and the proportion of individuals in each ontogenetic class and palm density were response variables. The total number of individuals (from seedlings to reproductives, of all species) monitored was 6 450 in 2005, 7 268 in 2006, and 8 664 in 2007. The densities of two palm species were not influenced by the size of the fragment, but the population density of A. aculeatissimum was dependent on the size of the fragment: there were more individuals in the bigger than in the smaller forest fragments. The population structure of A. aculeatissimum, E. edulis, and G. schottiana was not altered in the smaller fragments, except the infants of G. schottiana. The main point to be drawn from the results found in this study is that the responses of density and population structure seem not to be dependent on fragment size, except for one species that resulted more abundant in bigger fragments.

  6. The song of the Brazilian population of Humpback Whale Megaptera novaeangliae, in the year 2000: individual song variations and possible implications

    Directory of Open Access Journals (Sweden)

    Arraut Eduardo M.

    2004-01-01

    Full Text Available The song of the Brazilian population of the Humpback Whale Megaptera novaeangliae was studied in its breeding and calving ground, the Abrolhos Bank, Bahia, Brazil, from July to November 2000. Aural and spectral analyses of digital recordings were completed for approximately 20 song cycles, totaling 5 hours of song from 10 different recording events. We identified 24 note types, organized in five themes. All songs presented the same themes and the order in which they were sung did not vary. We registered the appearance of a note type and the disappearance of a phrase ending, which indicate that the song changed as the season progressed. Moreover, we detected individual variation in the way singers performed certain complex note types. As songs are transmitted culturally, it is likely that singers have different abilities to compose and/or learn new notes. If, as it has been previously suggested, 'new' songs are preferred to 'old' ones, these more able singers will be sending out information about their learning abilities that could be used by other whales to decide whether or not to interact with them.

  7. Influence of early life factors on body mass index trajectory during childhood: a population-based longitudinal analysis in the Western Brazilian Amazon.

    Science.gov (United States)

    Lourenço, Barbara H; Villamor, Eduardo; Augusto, Rosângela A; Cardoso, Marly A

    2015-04-01

    Low- to middle-income countries may experience the occurrence of a dual burden of under and overnutrition. To better understand the overall progression of body mass index (BMI) during childhood, we estimated average BMI-for-age z-score (BAZ) growth curves in a population-based longitudinal study of 255 children living in the Brazilian Amazon. Children were aged 0.1-5.5 years at recruitment (2003). We collected data on socio-economic and maternal characteristics, children's birthweight and infant feeding practices. Child anthropometric measurements were taken in 2003, 2007 and 2009. BAZ differences among categories of exposure variables were calculated at 6 and 12 months, and 2, 7 and 10 years. At baseline, the mean (standard deviation) age was 2.6 (1.4) years; 12.9% were overweight and 3.9% thin. After adjustment, mean BAZ estimates were mostly negative. Boys were close to the median value for BAZ until 12 months, whereas girls were below the median (P=0.05). Children from households above the wealth median were 0.36 z- and 0.49 z-less underweight than poorer children at 7 and 10 years, respectively (Pchildhood. Although excessive weight gain is a public health concern, it is critical to restrict inequities, while promoting healthier growth in developing countries.

  8. GSTT1,GSTM 1 and CYP2E1 genetic polymorphisms in gastric cancer and chronic gastritis in a Brazilian population

    Institute of Scientific and Technical Information of China (English)

    Jucimara Colombo; Andréa Regina Baptista Rossit; Alaor Caetano; Aldenis Albaneze Borim; Durval Wornrath; Ana Elizabete Silva

    2004-01-01

    AIM: To test the hypothesis that, in the Southeastern Brazilian population, the GSTT1, GSTM1 and CYP2E1polymorphisms and putative risk factors are associated with an increased risk for gastric cancer.METHODS: We conducted a study on 100 cases of gastric cancer (GC), 100 cases of chronic gastritis (CG), and 150controls (C). Deletion of the GSTT1 and GSTM1 genes was assessed by multiplex PCR. CYP2E1/PstI genotyping was performed using a PCR-RFLP assay.RESULTS: No relationship between GSTT1/GSTM1 deletion and the c1/c2 genotype of CYP2E1 was observed among the three groups. However, a significant difference between CG and C was observed, due to a greater number of GSTT1/GSTM1 positive genotypes in the CG group. The GSTT1 null genotype occurred more frequently in Negroid subjects, and the GSTM1 null genotype in Caucasians, while the GSTM1 positive genotype was observed mainly in individuals with chronic gastritis infected with H pylori.CONCLUSION: Our findings indicate that there is no obvious relationship between the GSTT1, GSTM1 and CYP2E1 polymorphisms and gastric cancer.

  9. Questioning the "melting pot": analysis of Alu inserts in three population samples from Uruguay.

    Science.gov (United States)

    Hidalgo, Pedro C; Mut, Patricia; Ackermann, Elizabeth; Figueiro, Gonzalo; Sans, Monica

    2014-01-01

    The way that immigrants integrate into recipient societies has been discussed for decades, mainly from the perspective of the social sciences. Uruguay, as other American countries, received diffferent waves of European immigrants, although the details of the process of assimilation, when it did occur, are unclear. In this study we used genetic markers to understand the process experienced by the Basques, one of the major migration waves that populated Uruguay, and their relation to other immigrants, as well as to Native American and African descendants. For this purpose, we analyzed the allele frequencies of 10 ALU loci (A25, ACE, APOA1, B65, D1, F13B, PV92, TPA25, HS2.43, and HS4.65) in three samples from Uruguay (two of Basque descendants, one of non-Basque descendants) from two locations: Montevideo and Trinidad. No departure from Hardy-Weinberg expectations was observed, with the exceptions of the APOA1 and D1 loci in the non-Basque descendants' samples. Our data show that the major genetic contribution in the three samples comes from Europe (78-88%), with minor African (10-15%) and Native American (0-10%) contributions. Genetic distances reveal that Basque descendants from Trinidad cluster with Europeans, whereas both Montevideo samples cluster together and are separate from other populations, showing two diffferent types of integration, related to the general characteristics of each regional population.

  10. Resampling: An improvement of importance sampling in varying population size models.

    Science.gov (United States)

    Merle, C; Leblois, R; Rousset, F; Pudlo, P

    2017-04-01

    Sequential importance sampling algorithms have been defined to estimate likelihoods in models of ancestral population processes. However, these algorithms are based on features of the models with constant population size, and become inefficient when the population size varies in time, making likelihood-based inferences difficult in many demographic situations. In this work, we modify a previous sequential importance sampling algorithm to improve the efficiency of the likelihood estimation. Our procedure is still based on features of the model with constant size, but uses a resampling technique with a new resampling probability distribution depending on the pairwise composite likelihood. We tested our algorithm, called sequential importance sampling with resampling (SISR) on simulated data sets under different demographic cases. In most cases, we divided the computational cost by two for the same accuracy of inference, in some cases even by one hundred. This study provides the first assessment of the impact of such resampling techniques on parameter inference using sequential importance sampling, and extends the range of situations where likelihood inferences can be easily performed.

  11. The prevalence of Nonsuicidal Self-Injury (NSSI in a representative sample of the German population

    Directory of Open Access Journals (Sweden)

    Paul L. Plener

    2016-10-01

    Full Text Available Abstract Background Non-suicidal self-injury (NSSI is a proposed new “condition for further study” in the DSM-5. To date no prevalence data has been available on this diagnostic entity from a representative sample of the general population. Methods A representative sample of the German population (N = 2509, mean age = 48.8 years, SD = 18.1, female 55.4 % completed the NSSI section of the German version of the Self-Injurious Thoughts and Behaviors Interview (SITBI-G. Results A history of NSSI at least once during lifetime was reported by 3.1 % of all participants, with higher lifetime prevalence rates in younger age groups. DSM-5 NSSI disorder criteria were met by 0.3 %. The most common function of NSSI was automatic negative reinforcement (e.g. to alleviate negative feelings. Conclusions To the best of our knowledge, this is the first study reporting rates for the proposed NSSI category in DSM-5 from a representative sample of the general population. In comparison to findings from community samples of adolescents, adults seem to have lower lifetime prevalence rates of NSSI, thus making it necessary to emphasize prevention and treatment efforts in younger age groups.

  12. PERSONAL NETWORK SAMPLING, OUTDEGREE ANALYSIS AND MULTILEVEL ANALYSIS - INTRODUCING THE NETWORK CONCEPT IN STUDIES OF HIDDEN POPULATIONS

    NARCIS (Netherlands)

    SPREEN, M; ZWAAGSTRA, R

    1994-01-01

    Populations, such as heroin and cocaine users, the homeless and the like (hidden populations), are among the most difficult populations to which to apply classic random sampling procedures. A frequently used data collection method for these hidden populations is the snowball procedure. The prerequis

  13. Sampling characteristics and calibration of snorkel counts to estimate stream fish populations

    Science.gov (United States)

    Weaver, D.; Kwak, Thomas J.; Pollock, Kenneth

    2014-01-01

    Snorkeling is a versatile technique for estimating lotic fish population characteristics; however, few investigators have evaluated its accuracy at population or assemblage levels. We evaluated the accuracy of snorkeling using prepositioned areal electrofishing (PAE) for estimating fish populations in a medium-sized Appalachian Mountain river during fall 2008 and summer 2009. Strip-transect snorkel counts were calibrated with PAE counts in identical locations among macrohabitats, fish species or taxa, and seasons. Mean snorkeling efficiency (i.e., the proportion of individuals counted from the true population) among all taxa and seasons was 14.7% (SE, 2.5%), and the highest efficiencies were for River Chub Nocomis micropogon at 21.1% (SE, 5.9%), Central Stoneroller Campostoma anomalum at 20.3% (SE, 9.6%), and darters (Percidae) at 17.1% (SE, 3.7%), whereas efficiencies were lower for shiners (Notropis spp., Cyprinella spp., Luxilus spp.) at 8.2% (SE, 2.2%) and suckers (Catostomidae) at 6.6% (SE, 3.2%). Macrohabitat type, fish taxon, or sampling season did not significantly explain variance in snorkeling efficiency. Mean snorkeling detection probability (i.e., probability of detecting at least one individual of a taxon) among fish taxa and seasons was 58.4% (SE, 6.1%). We applied the efficiencies from our calibration study to adjust snorkel counts from an intensive snorkeling survey conducted in a nearby reach. Total fish density estimates from strip-transect counts adjusted for snorkeling efficiency were 7,288 fish/ha (SE, 1,564) during summer and 15,805 fish/ha (SE, 4,947) during fall. Precision of fish density estimates is influenced by variation in snorkeling efficiency and sample size and may be increased with additional sampling effort. These results demonstrate the sampling properties and utility of snorkeling to characterize lotic fish assemblages with acceptable efficiency and detection probability, less effort, and no mortality, compared with traditional

  14. Cytogenetical and morphological features reveal significant differences among Venezuelan and Brazilian samples of Mugil curema (Teleostei: Mugilidae

    Directory of Open Access Journals (Sweden)

    Mauro Nirchio

    2005-03-01

    Full Text Available Karyotype of M. curema from the Gulf of Mexico and Brazil have been reported as possessing chromosome complement with 2n=28 and FN=48, whereas specimens from Venezuela has been reported as possessing a diploid number 2n=24 and a conserved FN (48. Although at first sight this variation suggests the presence of a chromosomal intraspecific (interpopulational variability, the possibility that we are dealing with two different species was examined. This work revisit the karyotypes of M. curema from Venezuela and Brazil, including new data on C-banding, and NOR localization, and compares morphologic characteristics of samples from both localities. Thus, besides diploid number, the constitutive heterochromatin distribution and NORs location, mark other differences between M. curema Cytotype 1 (2n=28; FN=48 and Cytotype 2 (2n=24; NF=48. Moreover, morphologic comparison revealed differences in the scale counts and pectoral fin rays: 35 scales in the middle body line and 15 pectoral fin rays in specimens possessing the karyotype 2n=28, compared with 37-39 scales in the middle body line and 17 pectoral fin rays in specimens with the karyotype 2n=24. These differences lead us to suggest that both cytotypes are not related merely to geographic polytipic variations but could correspond to different species.Os cariótipos de M. curema do Golfo do México e do Brasil possuem 2n=28 cromossomos e NF=48. Espécimes da Venezuela, entretanto, apresentam um número diplóide de 28 cromossomos e um NF conservado (48. Apesar desta variação sugerir, a princípio, uma variabilidade intraespecífica (interpopulacional, a possibilidade de estarmos perante diferentes espécies foi investigada. O presente trabalho re-analisa os cariótipos de M. curema da Venezuela e do Brasil, incluindo novos dados sobre bandamento C e localização das NORs e compara caracteres merísticos e morfométricos de amostras de ambas as localidades. Assim, junto com o número cromossômico, a

  15. Genotyping-by-sequencing approach indicates geographic distance as the main factor affecting genetic structure and gene flow in Brazilian populations of Grapholita molesta (Lepidoptera, Tortricidae)

    OpenAIRE

    Silva-Brandão, Karina Lucas; Oscar Arnaldo Batista Neto E Silva; Brandão, Marcelo Mendes; Omoto, Celso; Sperling, Felix A. H.

    2015-01-01

    The oriental fruit moth Grapholita molesta is one of the major pests of stone and pome fruit species in Brazil. Here, we applied 1226 SNPs obtained by genotyping-by-sequencing to test whether host species associations or other factors such as geographic distance structured populations of this pest. Populations from the main areas of occurrence of G. molesta were sampled principally from peach and apple orchards. Three main clusters were recovered by neighbor-joining analysis, all defined by g...

  16. Validação da Escala de Abordagens de Aprendizagem (EABAP em uma amostra Brasileira Validation of the Learning Approach Scale (LAS in a Brazilian sample

    Directory of Open Access Journals (Sweden)

    Cristiano Mauro Assis Gomes

    2011-01-01

    Full Text Available O presente estudo teve como objetivo validar a Escala de Abordagens de Aprendizagem (EABAP, construída para mensurar a dimensão Superficial e Profunda, em uma amostra brasileira. Fizeram parte da pesquisa 716 estudantes do ensino fundamental e médio de uma escola de Belo Horizonte. A análise fatorial exploratória foi utilizada para a identificação das dimensões e seleção dos melhores itens. Dos 27 itens estipulados, foram mantidos 17. A Análise Fatorial Confirmatória indicou a adequação da solução de um fator geral e de dois fatores específicos que representam a Abordagem Superficial e a Profunda (χ2=281,50; gl=116; CFI=0,94; GFI=0,95 e RMSEA=0,05. São discutidas implicações dos resultados para a teoria das abordagens, assim como algumas potencialidades do EABAP para a Psicologia Educacional.The main objective of the current study is to build and validate the Learning Approach Scale (LAS, which was developed to assess the deep and surface dimensions in a Brazilian sample. Seven hundred sixteen participants from junior and high schools in the city of Belo Horizonte took part in the research. The exploratory factor analysis was used to identify those dimensions and to select the best items. Only 17 items were kept from the 27 original ones. By the Confirmatory Factor Analysis it was possible to identify the adequacy of one general and two specific factors, the latter representing the deep and surface approaches (χ2=281.50; gl=116; CFI=0.94; GFI=0.95 and RMSEA=0.05. The implications of the results for the theory of approaches and the application of the scale in the Educational Psychology field are discussed.

  17. Analysis of Sampling Methodologies for Noise Pollution Assessment and the Impact on the Population.

    Science.gov (United States)

    Rey Gozalo, Guillermo; Barrigón Morillas, Juan Miguel

    2016-05-11

    Today, noise pollution is an increasing environmental stressor. Noise maps are recognised as the main tool for assessing and managing environmental noise, but their accuracy largely depends on the sampling method used. The sampling methods most commonly used by different researchers (grid, legislative road types and categorisation methods) were analysed and compared using the city of Talca (Chile) as a test case. The results show that the stratification of sound values in road categories has a significantly lower prediction error and a higher capacity for discrimination and prediction than in the legislative road types used by the Ministry of Transport and Telecommunications in Chile. Also, the use of one or another method implies significant differences in the assessment of population exposure to noise pollution. Thus, the selection of a suitable method for performing noise maps through measurements is essential to achieve an accurate assessment of the impact of noise pollution on the population.

  18. Analysis of Sampling Methodologies for Noise Pollution Assessment and the Impact on the Population

    Directory of Open Access Journals (Sweden)

    Guillermo Rey Gozalo

    2016-05-01

    Full Text Available Today, noise pollution is an increasing environmental stressor. Noise maps are recognised as the main tool for assessing and managing environmental noise, but their accuracy largely depends on the sampling method used. The sampling methods most commonly used by different researchers (grid, legislative road types and categorisation methods were analysed and compared using the city of Talca (Chile as a test case. The results show that the stratification of sound values in road categories has a significantly lower prediction error and a higher capacity for discrimination and prediction than in the legislative road types used by the Ministry of Transport and Telecommunications in Chile. Also, the use of one or another method implies significant differences in the assessment of population exposure to noise pollution. Thus, the selection of a suitable method for performing noise maps through measurements is essential to achieve an accurate assessment of the impact of noise pollution on the population.

  19. Y-chromosomal STR haplotypes in Inuit and Danish population samples

    DEFF Research Database (Denmark)

    Bosch, Elena; Rosser, Zoë H; Nørby, Søren;

    2003-01-01

    Nineteen Y-chromosomal short tandem repeats (STRs), DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS388, DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, DYS460, DYS461 and DYS462 were typed in Inuit (n=70) and Danish (n=62) population samples.......Nineteen Y-chromosomal short tandem repeats (STRs), DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS388, DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, DYS460, DYS461 and DYS462 were typed in Inuit (n=70) and Danish (n=62) population samples....

  20. Selection Component Analysis of Natural Polymorphisms using Population Samples Including Mother-Offspring Combinations, II

    DEFF Research Database (Denmark)

    Jarmer, Hanne Østergaard; Christiansen, Freddy Bugge

    1981-01-01

    Population samples including mother-offspring combinations provide information on the selection components: zygotic selection, sexual selection, gametic seletion and fecundity selection, on the mating pattern, and on the deviation from linkage equilibrium among the loci studied. The theory...... for the analysis of a sample consisting of adult individuals including combinations of a mother and one randomly chosen offspring in the case of n polymorphic autosomal loci with ma alleles at the ath locus, a = 1, 2,…, n, is developed for an organism with discrete non-overlapping generations. In this general...... analysis complete determination of the genotypes is assumed. The modifications in the analysis for the case n = 2 and m1 = m2 = 2 when the two double heterozygotes cannot be separated are discussed, and the analysis is illustrated by data from a population of Zoarces viviparus (a marine teleost)....

  1. Cytochrome P450 Drug Metabolizing Enzymes in Roma Population Samples: Systematic Review of the Literature.

    Science.gov (United States)

    Szalai, Renata; Hadzsiev, Kinga; Melegh, Bela

    2016-01-01

    The cytochrome P450 drug metabolizing enzymes are highly polymorphic and show inter-individual differences in variability in drug response, which varies widely also with ethnicity. This study aims to summarize the available data on genetic polymorphisms associated with cytochrome enzymes conducted on Roma populations. Our goal was to compare the frequency of the variant alleles, genotypes and predicted phenotypes with corresponding rates from other populations. We carried out a systematic review including the papers published on the pharmacogenetically relevant variants of cytochrome P450 genes related to Roma population. The study was performed using several articles, websites and databases, including PubMed, Ensembl, dbSNP, HapMap and 1000 Genomes Project. This review attempts to summarize and discuss our current knowledge about the frequency distribution of the ever investigated 20 allelic variants of 9 cytochrome genes (CYP1A2, CYP1B1, CYP2B6, CYP2C9, CYP2C19, CYP2C8, CYP2D6, CYP3A5, CYP4F2) in Roma DNA samples and compare them with other populations. Differences between Roma and Hungarian samples are reported for 7 variant genotypes. CYP2C9 *2/*3 and CYP2C19 *2/*2 genotypes showed more than 3-fold differences. Additional differences are displayed for allele frequency of 7 variants (rs762551, rs3745274, rs1058930, rs1065852, rs3892097, rs1057910 and rs4244285) in Roma population samples. The interethnic variability in clinically relevant genetic polymorphisms of drug metabolizing enzymes, which may explain distinct drug response, highlights the need to allow for the ancestry of participants in pharmacogenetic studies.

  2. A Homogeneous Stellar Sample for the Study of Metal-Rich Populations

    Directory of Open Access Journals (Sweden)

    M. Chávez

    2001-01-01

    Full Text Available We report on preliminary results of a project intended to construct a homogeneous spectroscopic data base of super metal-rich stars (SMR. The data base will serve as a tool for studies of old stellar populations. Using the flu x fitting method we have compared mid-resolution spectroscopic data of a sample o f SMR candidates with a grid of synthetic spectra and derived simultaneously the three leading atmospheric parameters (Teff, log {g}, [M/H].

  3. Accounting for randomness in measurement and sampling in studying cancer cell population dynamics.

    Science.gov (United States)

    Ghavami, Siavash; Wolkenhauer, Olaf; Lahouti, Farshad; Ullah, Mukhtar; Linnebacher, Michael

    2014-10-01

    Knowing the expected temporal evolution of the proportion of different cell types in sample tissues gives an indication about the progression of the disease and its possible response to drugs. Such systems have been modelled using Markov processes. We here consider an experimentally realistic scenario in which transition probabilities are estimated from noisy cell population size measurements. Using aggregated data of FACS measurements, we develop MMSE and ML estimators and formulate two problems to find the minimum number of required samples and measurements to guarantee the accuracy of predicted population sizes. Our numerical results show that the convergence mechanism of transition probabilities and steady states differ widely from the real values if one uses the standard deterministic approach for noisy measurements. This provides support for our argument that for the analysis of FACS data one should consider the observed state as a random variable. The second problem we address is about the consequences of estimating the probability of a cell being in a particular state from measurements of small population of cells. We show how the uncertainty arising from small sample sizes can be captured by a distribution for the state probability.

  4. Stellar population properties for a sample of hard X-ray AGNs

    CERN Document Server

    Morelli, L; Masetti, N; Parisi, P; Landi, R; Maiorano, E; Minniti, D; Galaz, G

    2013-01-01

    The aim of this paper is to study the stellar population of galaxies hosting an active galactic nucleus (AGN). We studied a sub-sample of hard X-ray emitting AGNs from the INTEGRAL and Swift catalogs which were previously identified and characterized through optical spectroscopy. Our analysis provides complementary information, namely age and metallicity, which is necessary to complete the panoramic view of such interesting objects. We selected hard X-ray emitting objects identified as AGNs by checking their optical spectra in search for absorption lines suitable for the stellar population analysis. We obtained a final sample consisting of 20 objects with redshift lower than 0.3. We used the full-spectrum fitting method and, in particular, the penalized pixel one applying the PPXF code. After masking all the regions affected by emission lines, we fitted the spectra with the MILES single stellar population templates and we derived mass-weighted ages and metallicities. Most of the objects in our sample show an ...

  5. Population data for 12 Y-chromosome STR loci in a sample from Honduras.

    Science.gov (United States)

    Matamoros, Mireya; Yurrebaso, Iñaki; Gusmão, Leonor; García, Oscar

    2009-09-01

    Haplotype, allele frequencies and population data of 12 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 were determined from a sample of 128 unrelated male individuals from Honduras, Central America. A total of 112 haplotypes were identified by the 12 Y-STR loci of which 98 were unique. The haplotype diversity (98.99%) and the proportion of different haplotypes (87.50%) were estimated. Genetic distances were calculated between Honduras and other populations from Southern and Central America, Europe and Africa. The analysis of a Multi Dimensional Scaling (MDS) plot, based on pairwise R(ST) genetic distances, allowed to conclude that Honduras is highly differentiated from the African samples (0.343Honduras showed a lower genetic distance to the European cluster (composed by European and South American general population samples from Brazil, Argentina, Colombia and Venezuela) than to the Central American cluster (Mexico and El Salvador).

  6. Validation of the anti-bacteremic efficacy of an essential oil rinse in a Brazilian population: a cross-over study

    Directory of Open Access Journals (Sweden)

    José Roberto Cortelli

    2012-10-01

    Full Text Available This cross-over study was conducted to assess the germ-killing efficacy of an essential oil mouthrinse (EOM by determining the blood levels of microorganisms associated with induced bacteremia and investigating the prevalence of this event in Brazilians with mild-to-moderate gingivitis. Thirty four (31.19% subjects positive for bacteremia induced by chewing a ration of apple were enrolled out of 109 screened subjects (50 males and 59 females. A difference of at least 10 colony forming units between the pre- and post-insult blood samples was defined as a positive result. For the following two weeks patients underwent a toothbrush plus fluoride dentifrice normalization period, and were then scheduled for the Phase I protocol as follows. At baseline I, subjects were instructed to chew a new apple ration, had new blood samples taken before and after this oral stimulus, and were randomly assigned to an experimental essential oil (n = 17 or placebo (P mouthrinse (n = 17 treatment for 2 weeks. These procedures were repeated at the end of Phase I and then followed by a two-week wash-out period (tooth brushing with fluoride dentifrice. Bacteremia was again induced at baseline and at the end of Phase II, when subjects were crossed-over to the other EOM or placebo groups. Bacterial count differences between baseline and 2-week post-treatment (EOM versus P in the blood samples collected were assessed by analysis of covariance. Mean aerobic counts decreased by 45.8%, whereas mean anaerobic counts decreased by 63.3% after EOM treatment. After the P treatment, aerobic bacteria increased by 28.4% and anaerobic bacteria decreased by 18.5%. This study validated this novel methodology and showed that the germ-killing action of EOM significantly reduced bacteremia.

  7. A two-stage cluster sampling method using gridded population data, a GIS, and Google EarthTM imagery in a population-based mortality survey in Iraq

    Directory of Open Access Journals (Sweden)

    Galway LP

    2012-04-01

    Full Text Available Abstract Background Mortality estimates can measure and monitor the impacts of conflict on a population, guide humanitarian efforts, and help to better understand the public health impacts of conflict. Vital statistics registration and surveillance systems are rarely functional in conflict settings, posing a challenge of estimating mortality using retrospective population-based surveys. Results We present a two-stage cluster sampling method for application in population-based mortality surveys. The sampling method utilizes gridded population data and a geographic information system (GIS to select clusters in the first sampling stage and Google Earth TM imagery and sampling grids to select households in the second sampling stage. The sampling method is implemented in a household mortality study in Iraq in 2011. Factors affecting feasibility and methodological quality are described. Conclusion Sampling is a challenge in retrospective population-based mortality studies and alternatives that improve on the conventional approaches are needed. The sampling strategy presented here was designed to generate a representative sample of the Iraqi population while reducing the potential for bias and considering the context specific challenges of the study setting. This sampling strategy, or variations on it, are adaptable and should be considered and tested in other conflict settings.

  8. Inventory in a Brazilian Sample

    Directory of Open Access Journals (Sweden)

    Taciano L. Milfont

    2008-01-01

    Full Text Available Éste artículo evalúa la validez de constructo y la validez discriminante del Inventario de Perspectiva Temporal de Zimbardo (Zimbardo Time Perspective Inventory, ZTPI de una muestra de 247 estudiantes brasileños. La estructura de cinco-factores de ZTPI, definidos por Pasado-Negativo, Presente-Hedonístico, Futuro, Pasado- Positivo y Presente-Fatalista, provee un ajuste aceptable de los datos, y pareció ser mejor estadísticamente que el ajuste de los modelos alternativos. El Presente-Hedonístico fue positivamente correlacionado con el consumo de alcohol y negativamente con la religiosidad, el Futuro fue correlacionado positivamente con la preocupación por la salud y negativamente con el consumo de alcohol, y el Pasado-Positivo fue positivamente correlacionado con el uso del reloj. Los resultados encontrados concuerdan con los estudios anteriores, indicando que las cinco dimensiones de perspectiva del tiempo pueden ser identificadas cognitivamente. Además los patrones de correlación con otras variables son comparables a través de otras culturas.

  9. Ochratoxin A in Brazilian green coffee

    Directory of Open Access Journals (Sweden)

    LEONI Luís A.B.

    2001-01-01

    Full Text Available Ochratoxin A is a nephrotoxic, teratogenic and imunotoxic compound produced by Aspergillus and Penicillium spp. It is a suspected carcinogen to humans and it is carcinogenic to rats. Recently it has drawn attention because it has been found in coffee and it has been the object of regulation by coffee importing countries. Brazil is the largest coffee producing country and its largest consumer. In order to conduct an initial assessment of the situation of the coffee produced in the country and offered to its population, one hundred and thirty two samples of Brazilian green coffee from 5 producing states (Minas Gerais, Paraná, São Paulo, Espírito Santo and Bahia and destined for the home market, were collected at sales points at the cities of Londrina and Santos, Brazil, and analyzed for ochratoxin A. The toxin was isolated in immunoaffinity columns and quantified by HPLC with florescence detection. The limit of detection was 0.7ng/g and the average RSD for duplicates of the samples was 11%. Twenty seven samples were found contaminated with the toxin and the average concentration for the contaminated samples was 7.1ng/g ochratoxin A. Neither the total number of defects nor the number of specific defects according to the Brazilian coffee classification system (black, partly -- black, sour, stinkers-black, stinkers-green, pod beans showed any relation to the contamination of the samples with ochratoxin A.

  10. Measuring population fluctuation of jatropha stem-borer [Cophes notaticeps (Marshall)] in the Brazilian Cerrado using a new trap.

    Science.gov (United States)

    Oliveira, C M; Silva, C N; Frizzas, M R; Dianese, A C

    2017-02-10

    This study aimed to monitor the population fluctuation of Cophes notaticeps (Marshall) (Coleoptera: Curculionidae) in a jatropha (Jatropha curcas L.) plantation in the Federal District, Brazil, through the use of a new trap, combined with different attractive lures and trap colors. The study was conducted at Embrapa Cerrados (Planaltina/DF) in a field trial with 720 plants. The new trap, named CPAC16, was made with PVC pipes (100 mm in diameter) of about 40 cm in length, with a window (8 × 10 cm2) in its center to enable insect access. A lure compartment was fixed on the inside of the trap`s top, and a pot, containing water and detergent, was placed at its bottom to collect the insects. The traps were painted in red, yellow, blue and green. Molasses, ethanol and pineapple were used as attractive lures. The traps were in the field trial area between May 2013 and April 2014 and the insects were collected weekly. The incidence of plants presenting damage caused by C. notaticeps was evaluated in the beginning and at the end of the study. The CPAC16 trap proved efficient in monitoring C. notaticeps. About 3494 of C. notaticeps adults were collected during the study. There were no significant differences among traps painted with different colors. The most attractive lure was molasses collecting 75.2% (n = 2627) of the specimens. Although the population peak occurred in December (n = 1162), C. notaticeps were collected throughout the year. The incidence of plants attacked by C. notaticeps ranged from 66.4% (start) to 100% (end).

  11. Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis.

    Science.gov (United States)

    Islam, Monsur; Natu, Rucha; Larraga-Martinez, Maria Fernanda; Martinez-Duarte, Rodrigo

    2016-05-01

    Here, we report on an enrichment protocol using carbon electrode dielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 10(6)-10(2) cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 ± 23.7 times was achieved when the sample flow rate was 10 μl/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved.

  12. O envelhecimento da população brasileira: um enfoque demográfico The aging process in the Brazilian population: a demographic approach

    Directory of Open Access Journals (Sweden)

    José Alberto Magno de Carvalho

    2003-06-01

    Full Text Available Contrariamente ao indicado pelo senso comum, o processo de envelhecimento populacional, tal como observado até hoje, é resultado do declínio da fecundidade, e não da mortalidade. O envelhecimento populacional iniciou-se no final do século XIX em alguns países da Europa Ocidental, espalhou-se pelo resto do Primeiro Mundo, no século passado, e se estendeu, nas últimas décadas, por vários países do Terceiro Mundo, inclusive o Brasil. No caso brasileiro, observou-se, a partir do final dos anos 60, rapidíssima e generalizada queda da fecundidade, e haverá, conseqüentemente, um célere processo de envelhecimento da população. Este processo será, necessariamente, mais rápido e com mudanças estruturais, demograficamente falando, mais profundas do que nos países do Primeiro Mundo por duas razões: o declínio da fecundidade, no País, deu-se em um ritmo maior e origina-se de uma população mais jovem do que aquela dos países desenvolvidos.Contrary to common sense, the population aging process is due to the decline in fertility rather than mortality. The aging process began around the end of the 19th century in a number of Western European countries, expanded to the rest of the so-called First World over the past century, and reached several Third World countries afterwards, including Brazil over the last decades. In the Brazilian case, a sharp and widespread fertility decline began by the end of the 1960s, and an accelerated population aging process can thus be expected. This process will necessarily be faster and with deeper structural changes, demographically speaking, than in First World countries, for two reasons: the fertility decline in Brazil was faster, and it took place in a population with a younger age structure.

  13. Sonolência excessiva diurna na população geral de um município brasileiro Excessive daytime sleepiness in the general population of Brazilian city

    Directory of Open Access Journals (Sweden)

    José Carlos Souza

    2008-01-01

    Full Text Available OBJETIVO: Buscou-se avaliar a prevalência da sonolência excessiva diurna (SED na população geral de um município brasileiro. MÉTODO: Foram feitas 198 entrevistas domiciliares entre os adultos, em amostra representativa da população geral de Ribeirão do Largo, BA. A amostragem foi aleatória simples. Tinham SED as pessoas com 11 ou mais pontos na Escala de Sonolência de Epworth (ESE. Usaram-se os testes de qui-quadrado, Fisher e ANOVA; nível de significância 5%. RESULTADOS: Tinham SED 21,5% da população (DP = 2,9%; IC 15,8% a 27,2%; não houve associação significativa entre SED e idade (p = 0,924, nem IMC (p = 0,197, sexo (p = 0,095, instrução (p = 0,700, estado civil (p = 0,414 e uso de hipnóticos (p = 0,176. Houve associação com o despertar precoce (p = 0,046. CONCLUSÃO: Foi alta a prevalência de SED na população estudada.OBJECTIVE: To assess the prevalence of excessive daytime sleepiness (ESD in general population of a Brazilian city. METHODS: It was determined by means of 198 home interviews of adults, in a representative sample of Ribeirão do Largo city, Brazil. The random sample was simple. ESD was considered in those with index 11 or more in the Epworth Sleepiness Scale (ESS. Statistics used chi-square, Fisher and ANOVA; the significance level was 5%. RESULTS: The prevalence of ESD was 21.5% of population (SD = 2.9%; CI 15.8%-27.2%. No significant association was found between ESD and age (p=0.924, nor with body mass index (p=0.197, sex (p=0.095, schooling (p=0,700, marital status (p=0.414 and the use of hypnotics (p=0.176. There was significant association with early awakening (p=0.046. CONCLUSION: There was a high prevalence of ESD in the population.

  14. Prevalência e fatores associados a sintomas depressivos em adultos do sul do Brasil: estudo transversal de base populacional Prevalence of depressive symptons and associated factors among southern Brazilian adults: cross-sectional population-based study

    Directory of Open Access Journals (Sweden)

    Airton José Rombaldi

    2010-12-01

    Full Text Available Com o objetivo de identificar a prevalência de sintomas depressivos e examinar fatores associados em uma população adulta do sul do Brasil, foi realizado um estudo transversal de base populacional, incluindo 972 indivíduos, de ambos os sexos, idade entre 20 e 69 anos, moradores na zona urbana da cidade de Pelotas, Rio Grande do Sul. A seleção amostral teve os setores censitários do município como unidades amostrais primárias e os domicílios como unidades secundárias. O questionário incluiu variáveis socioeconômicas, comportamentais e nutricionais. As prevalências dos sintomas depressivos tristeza, ansiedade, falta de energia, falta de disposição, pensar no passado e preferir ficar em casa, na população de Pelotas, foram, respectivamente, de 29,4%, 57,6%, 37,4%, 40,4%, 33,8% e 54,3%. Concluiu-se que as prevalências de sintomas depressivos foram elevadas e os indivíduos do sexo feminino, idade avançada, fumantes e obesos mostraram-se associadas à maioria dos desfechos. A depressão é um importante problema de saúde publica e o conhecimento de como a sintomatologia depressiva se distribui na população e os fatores associados à sua presença podem ajudar no melhor entendimento da fenomenologia dos transtornos depressivos e a traçar estratégias de prevenção e intervenção.To identify the prevalence of depressive symptoms and examine associated factors in a Southern Brazilian adult population, a cross-sectional population-based study was carried out, including 972 subjects, men and women, aged 20 to 69 years, living in the urban area of Pelotas, Rio Grande do Sul. The sampling strategy relied on the census tracts of the city as primary sampling units and households as the secondary units. The questionnaire included socio-demographic, behavioral and nutritional variables. The prevalence of sadness, anxiety, loss of energy, lack of will to do things, thinking about the past, and wishing to stay at home were 29.4%, 57

  15. HLA-B*44 Is Associated with Dengue Severity Caused by DENV-3 in a Brazilian Population

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    Liciana Xavier Eurico de Alencar

    2013-01-01

    Full Text Available Human leukocyte antigen (HLA alleles have been correlated with susceptibility or resistance to severe dengue; however, few immunogenetic studies have been performed in Latin American (LA populations. We have conducted immunogenetic studies of HLA class I and II alleles in a cohort of 187 patients with DENV-3 infection and confirmed clinical diagnosis of either severe dengue, known as dengue hemorrhagic fever (DHF, or the less severe form, dengue fever (DF, in Recife, Pernambuco, Brazil. An association analysis was performed using Fisher’s association test, with odds ratios (ORs calculated using conditional maximum likelihood estimates. HLA-B*44 (P=0.047, OR = 2.025, 95% CI = 0.97–4.24 was found to be associated with increased susceptibility to DHF in response to DENV-3 infection. In addition, HLA-B*07 (P=0.048, OR = 0.501, one-sided 95% CI = 0–0.99 and HLA-DR*13 (P=0.028, OR = 0.511, one-sided 95% CI = 0–0.91 were found to be associated with resistance to secondary dengue infection by DENV-3. These results suggest that HLA-B*44 supertype alleles and their respective T-cell responses might be involved in susceptibility to severe dengue infections, whereas the HLA-B*07 supertype alleles and DR*13 might be involved in cross-dengue serotype immunity.

  16. Falls and self-assessment of eyesight among elderly people: a population-based study in a south Brazilian municipality.

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    Nunes, Bruno Pereira; de Oliveira Saes, Mirelle; Siqueira, Fernando Vinholes; Tomasi, Elaine; Silva, Suele Manjourany; da Silveira, Denise Silva; Soares, Mariangela Uhlmann; Facchini, Luiz Augusto; Thumé, Elaine

    2014-01-01

    This paper seeks to verify the association between falls and self-assessment of visual acuity in elderly people by means of a cross-sectional population-based study involving 1593 elderly people (aged 60 or over) from the urban zone of the municipality of Bagé-RS. Poisson regression was used for association analysis. Fall prevalence in the last year was 28.0% (95%CI: 25.8; 30.2), with 45.0% of these having suffered two or more falls in the same period. Elderly people self-assessing their eyesight as bad/very poor (10.0%) or regular (33.3%) showed a linear increase in fall occurrence when compared to individuals who considered their eyesight to be good/excellent. Self-assessment of eyesight showed itself to be an important factor associated with the occurrence of falls. This results entails the need to make progress with tracing elderly people with eyesight difficulties and its possible impact on actions to prevent the occurrence of falls.

  17. Urinary concentrations of pyrethroid metabolites in the convenience sample of an urban population of Northern Poland.

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    Wielgomas, Bartosz; Nahorski, Wacław; Czarnowski, Wojciech

    2013-06-01

    Urinary concentrations of pyrethroid metabolites were measured in the first void urine samples collected from 132 healthy people living in the Gdańsk region of Northern Poland in 2010 and 2011. Four metabolites of synthetic pyrethroids: cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acids (cis-, trans-Cl2CA), cis-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (Br2CA) and 3-phenoxybenzoic acid (3-PBA) were simultaneously liquid-liquid extracted, derivatized with hexafluoroisopropanol and analyzed by a gas chromatography ion-trap mass spectrometry. All the analytes were detected and quantified in the samples with various frequency, 3-phenoxybenzoic being the most often (80%) and the others less frequently (7-11%). Distribution of 3-PBA concentrations followed log-normal model, the mean concentration of 3-phenoxybenzoic acid: 0.393 μg/L (0.327 μg/g creatinine) is similar to those of the other general populations in various regions of the world. Neither sex nor age were predictors of urinary 3-PBA. Our findings suggest wide exposure to pyrethroid insecticides in the Polish general population. There is a continuous need to further study the exposure to synthetic pyrethroids among the general population since there is a strong, increasing trend in their usage.

  18. Fatores de risco para o prolapso genital em uma população brasileira Risk factors for genital prolapse in a Brazilian population

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    Andrea Moura Rodrigues

    2009-01-01

    Full Text Available OBJETIVO: avaliar os fatores de risco para o desenvolvimento de prolapso genital na população brasileira. MÉTODOS: estudo caso-controle envolvendo 316 pacientes que foram submetidas a estadiamento de prolapso, utilizando-se o sistema de quantificação de prolapso dos órgãos pélvicos. As pacientes foram divididas em dois grupos. No Grupo Caso, foram incluídas 107 pacientes com prolapso nos estádios III ou IV, e no Controle, 209 mulheres com estádios 0 ou I. As mulheres selecionadas respondiam à anamnese na qual eram questionadas sobre a presença de possíveis fatores de risco para prolapso genital, tais como: idade, idade da menopausa, paridade, tipos de parto (vaginal, cesariana ou fórcipe, ocorrência de macrossomia fetal, história familiar em parentes de primeiro grau de distopia genital, tosse crônica e constipação intestinal. RESULTADOS: as variáveis que se mostraram diferentes entre os grupos foram: idade, índice de massa corpórea, paridade, número de partos vaginais, de cesarianas, de partos fórcipe, peso do recém-nascido e história familiar positiva para prolapso. Raça, idade da menopausa, tosse crônica e constipação intestinal não se mostraram diferentes entre os grupos. Após a regressão logística, somente três variáveis se apresentaram como fatores de risco independentes: presença de pelo menos um parto vaginal, macrossomia fetal e história familiar positiva. A cesariana se mostrou como fator protetor. CONCLUSÕES: na população brasileira, os fatores de risco independentes para prolapso foram a presença de pelo menos um parto normal, macrossomia fetal e história familiar positiva para distopia.PURPOSE: to evaluate risk factors for the development of genital prolapse in the Brazilian population. METHODS: case-control study involving 316 patients submitted to prolapse staging, according to the pelvic organ prolapse quantification system. The patients were divided into two groups: in the Case Group

  19. Relationship between Tasks Performed, Personality Traits, and Sleep Bruxism in Brazilian School Children - A Population-Based Cross-Sectional Study

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    Serra-Negra, Junia Maria; Paiva, Saul Martins; Abreu, Mauro Henrique; Flores-Mendoza, Carmen Elvira; Pordeus, Isabela Almeida

    2013-01-01

    Background Tasks can be instruments of stress and may affect the health of children. Sleep bruxism is a multifactorial sleep-related movement disorder that affects children and adults. The aim of the present study was to analyze the association between children’s tasks, personality traits and sleep bruxism. Methods And Findings A cross-sectional, population-based study of 652 randomly selected Brazilian schoolchildren (52% of whom were female), aged from 7 to 10 years was conducted in the city of Belo Horizonte, Brazil. A questionnaire based on criteria proposed by the American Academy of Sleep Medicine (AASM) was completed by parents. In addition, the Neuroticism and Responsibility sub-scales of the Big Five Questionnaire for Children (BFQ-C) were administered to the children. Psychological tests were administered and evaluated by psychologists. The Social Vulnerability Index from the city council database was used to determine the social classification of the families. Chi-square and Poisson regression statistical tests were used with a 95% confidence interval. The majority of families were classified as having low social vulnerability (61.3%), whereas, 38.7% were classified as having high social vulnerability. Regarding extracurricular activities, the majority of girls performed household work (56.4%) and some artistic activity (51.3%) while sporting activities were most common among boys (61%). The results of the Poisson regression model indicated that sleep bruxism was most prevalent in children who scored highly in the Neuroticism sub-scale, and who frequently performed household tasks. Conclusion Children whose personality domain has a high level of Neuroticism and who perform household chores imposed by the family are more vulnerable to sleep bruxism. PMID:24244614

  20. Populations analysis of the Brazilian Sharpnose Shark Rhizoprionodon lalandii (Chondrichthyes: Carcharhinidae on the São Paulo coast, Southern Brazil: inferences from mt DNA sequences

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    Fernando Fernandes Mendonça

    2009-06-01

    Full Text Available Sharks of the genus Rhizoprionodon can be considered some of the most important predators along the trophic coastal marine ecosystems and represent an important economic resource for the small-scale fisheries, especially on the Brazilian coastline. In order to analyze the population structure of the shark Rhizoprionodon lalandii of São Paulo, Southeastern coast of Brazil, levels of genetic diversity were identified by nucleotide sequence analyses of the mitochondrial DNA control region. The results obtained from this study present moderate values of haplotype diversity and low nucleotide diversity. Although the AMOVA tests (ΦST = 0.08394, P Tubarões do gênero Rhizoprionodon são considerados predadores de grande importância ao longo da cadeia trófica nos ecossistemas costeiros e marinhos, também representando um importante recurso econômico para a pesca, especialmente no litoral brasileiro. A fim de analisar a estrutura populacional do tubarão Rhizoprionodon lalandii no litoral de São Paulo, sudeste do Brasil, foram identificados os níveis de diversidade genética a partir da análise de sequências nucleotídicas da região controladora do DNA mitocondrial. Os dados obtidos neste estudo apresentam valores moderados de diversidade haplotípica e baixos índices de diversidade nucleotídica. Embora os testes de AMOVA (ΦST = 0,08394, P < 0,01 tenham revelado uma pequena diferença entre as amostras estudadas, evidências sobre a ocorrência de estruturação populacional não foram encontradas o que pode representar uma característica geral para tubarões vivendo em áreas costeiras.

  1. The effect of combined polymorphisms in chemokines and chemokine receptors on the clinical course of HIV-1 infection in a Brazilian population

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    Valdimara Corrêa Vieira

    2011-06-01

    Full Text Available Polymorphisms in genes that encode chemokines or their receptors can modulate susceptibility to human immunodeficiency virus (HIV infection and disease progression. The objective of this study was to assess the frequency of polymorphisms CCR5-Δ32, CCR2-64I, CCR5-59029A and SDF1-3'A and their role in the course of HIV infection in a Southern Brazilian population. Clinical data were obtained from 249 patients for an average period of 6.4 years and genotypes were determined by standard polymerase chain reaction (PCR and PCR-restriction fragment length polymorphism. Survival analyses were conducted for three outcomes: CD4+ T-cell counts below 200 cells/µL, acquired immune deficiency syndrome (AIDS or death. The frequency of the polymorphisms CCR5-Δ32, CCR2-64I, CCR5-59029A and SDF1-3'A were 0.024, 0.113, 0.487 and 0.207, respectively. CCR5-Δ32 was associated with a reduction in the risk for CD4+ T-cell depletion and with an increased risk for death after AIDS diagnosis. CCR2-64I was associated with a reduction in the risk for developing AIDS. SDF1-3'A was also associated with decreased risk for AIDS, but its effect was only evident when CCR2-64I was present as well. These results highlight the possibility of using these markers as indicators for the prognosis of disease progression and provide evidence for the importance of analysing the effects of gene polymorphisms in a combined fashion.

  2. Relationship between tasks performed, personality traits, and sleep bruxism in Brazilian school children--a population-based cross-sectional study.

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    Junia Maria Serra-Negra

    Full Text Available BACKGROUND: Tasks can be instruments of stress and may affect the health of children. Sleep bruxism is a multifactorial sleep-related movement disorder that affects children and adults. The aim of the present study was to analyze the association between children's tasks, personality traits and sleep bruxism. METHODS AND FINDINGS: A cross-sectional, population-based study of 652 randomly selected Brazilian schoolchildren (52% of whom were female, aged from 7 to 10 years was conducted in the city of Belo Horizonte, Brazil. A questionnaire based on criteria proposed by the American Academy of Sleep Medicine (AASM was completed by parents. In addition, the Neuroticism and Responsibility sub-scales of the Big Five Questionnaire for Children (BFQ-C were administered to the children. Psychological tests were administered and evaluated by psychologists. The Social Vulnerability Index from the city council database was used to determine the social classification of the families. Chi-square and Poisson regression statistical tests were used with a 95% confidence interval. The majority of families were classified as having low social vulnerability (61.3%, whereas, 38.7% were classified as having high social vulnerability. Regarding extracurricular activities, the majority of girls performed household work (56.4% and some artistic activity (51.3% while sporting activities were most common among boys (61%. The results of the Poisson regression model indicated that sleep bruxism was most prevalent in children who scored highly in the Neuroticism sub-scale, and who frequently performed household tasks. CONCLUSION: Children whose personality domain has a high level of Neuroticism and who perform household chores imposed by the family are more vulnerable to sleep bruxism.

  3. Analysis of food advertisements on cable television directed to children based on the food guide for the Brazilian population and current legislation

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    Soraya da Rocha BRITTO

    Full Text Available ABSTRACT Objective: This study examined food product advertisements directed to children and aired on closed television channels in Brazil, according to the types of foods and beverages advertised and the advertising content. Methods: A descriptive study was conducted on the adequacy of food commercials directed to children and aired by six pay-television broadcasters according to two parameters: "The Food Guide for the Brazilian Population, 2014", and the National Council on Children's and Adolescents' Rights Resolution nº 163 of 2014 about food advertising. The advertisements were recorded in July 2015, at different times and days of the week. Results: One hundred and sixty-two hours were recorded, registering 3,468 commercials: 1,850 were related to internal programming and 1,618 advertised toys (22.15%, food and beverages (5.61%, applications for electronic devices (5.58%, and entertainment/events (5.56%. The Fisher exact test showed fewer number of food commercials compared to other types of commercials (p<0.001. The main food items advertised by all television stations were ultraprocessed foods and no advertisements of fresh food were observed (p<0.001. Most of the food commercials (64.30% used children's language and characters; 43.00% used songs in children's voices, and 21.40% linked gift distribution to food. Conclusion: The number of food commercials observed was lower than in previous national studies. However, the advertisements did not follow current legislation, indicating the abuse of marketing communication to children. More effective public policies and the respect and enforcement of legislation on child advertising could protect children from high consumption of ultraprocessed foods.

  4. Personality factors and suicide risk in a representative sample of the German general population.

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    Victor Blüml

    Full Text Available OBJECTIVE: Previous research has shown an association between certain personality characteristics and suicidality. Methodological differences including small sample sizes and missing adjustment for possible confounding factors could explain the varying results. The aim of this study was to assess the impact of the Big Five personality dimensions on suicidality in a representative population based sample of adults. METHOD: Interviews were conducted in a representative German population-based sample (n=2555 in 2011. Personality characteristics were assessed using the Big Five Inventory-10 (BFI-10 and suicide risk was assessed with the Suicidal Behaviors Questionnaire-Revised (SBQ-R. Multivariate logistic regression models were calculated adjusting for depression, anxiety, and various sociodemographic variables. RESULTS: Neuroticism and openness were significantly associated with suicide risk, while extraversion and conscientiousness were found to be protective. Significant sex differences were observed. For males, extraversion and conscientiousness were protective factors. Neuroticism and openness were found to be associated with suicide risk only in females. These associations remained significant after adjusting for covariates. CONCLUSION: The results highlight the role of personality dimensions as risk factors for suicide-related behaviors. Different personality dimensions are significantly associated with suicide-related behaviors even when adjusting for other known risk factors of suicidality.

  5. Morphologic Patterns of Lip Prints in a Sample of Croatian Population

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    Šimović, Marija; Pavušk, Ivan; Muhasilović, Senad

    2016-01-01

    Purpose Cheiloscopy deals with the study of elevations and depressions which form a characteristic pattern on the external surface of the lips. Lip grooves are considered to be unique and analogous to the fingerprint. The aim of the research was to determine the type of grooves on healthy lips of men and women using lip prints in a sample of Croatian population, to determine whether there is a statistically significant difference between men and women in the types of grooves and to determine whether there are any differences between male and female lip prints. Material and methods A randomly selected sample of Croatian population consisted of 40 male and 50 female subjects. The samples of lip grooves were classified according to Tsuchihashi classification. Results The research has shown that most women in Croatia belong to Type 2 (40.0%), followed by Type 1 (34.0%), Type 3 (12.0%), Type 4 (8.0%), and Type 5 (6.0%) which is of less importance. Most Croatian men belong to Type 3 (35.0%) and Type 2 (25.0%). A small number of men belong to Type 4 (15.0%), Type 5 (12.5%) and Type1 (12.5%). Conclusion There was a statistically significant difference between men and women regarding the types of lip grooves.

  6. Population sampling of the golden mussel, Limnoperna fortunei (Dunker, 1857, based on artificial ceramic substrate

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    Paulo Eduardo Aydos Bergonci

    2009-09-01

    Full Text Available The ceramic substrate (21cm in length, 6cm in width and 1.3cm in depth was tested for the Limnoperna fortunei population, sampling at two localities in the Jacuí delta (Jacuí Canal (Canal do Jacuí – CJ and Port Docks (Cais do Porto – PO in Rio Grande do Sul state, Brazil. The individuals were quantified through the superimposition of a squared (1cm2 and segmented (sI, sII e sIII sheet on the substrate. Using Kruskal-Wallis and Mann-Whitnney, the recruit and adult average densities were compared in each segment, surface (smooth and pipe-shaped and sampling locality (α = 0.05. In CJ, the extreme and intermediate (adult segments differed statistically (p < 0.0001, as well as the ceramic substrate surfaces (recruits (p = 0.04. The recruit and adult densities between the CJ and PO localities also differed between themselves (p < 0.0001. The method was efficient for the invasive population sampling.

  7. Estimating ancestral proportions in a multi-ethnic US sample: implications for studies of admixed populations

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    Levran Orna

    2012-07-01

    Full Text Available Abstract This study was designed to determine the ancestral composition of a multi-ethnic sample collected for studies of drug addictions in New York City and Las Vegas, and to examine the reliability of self-identified ethnicity and three-generation family history data. Ancestry biographical scores for seven clusters corresponding to world major geographical regions were obtained using STRUCTURE, based on genotypes of 168 ancestry informative markers (AIMs, for a sample of 1,291 African Americans (AA, European Americans (EA, and Hispanic Americans (HA along with data from 1,051 HGDP-CEPH ‘diversity panel’ as a reference. Self-identified ethnicity and family history data, obtained in an interview, were accurate in identifying the individual major ancestry in the AA and the EA samples (approximately 99% and 95%, respectively but were not useful for the HA sample and could not predict the extent of admixture in any group. The mean proportions of the combined clusters corresponding to European and Middle Eastern populations in the AA sample, revealed by AIMs analysis, were 0.13. The HA subjects, predominantly Puerto Ricans, showed a highly variable hybrid contribution pattern of clusters corresponding to Europe (0.27, Middle East (0.27, Africa (0.20, and Central Asia (0.14. The effect of admixture on allele frequencies is demonstrated for two single-nucleotide polymorphisms (118A > G, 17 C > T of the mu opioid receptor gene (OPRM1. This study reiterates the importance of AIMs in defining ancestry, especially in admixed populations.

  8. A genome-wide association study of neuroticism in a population-based sample.

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    Calboli, Federico C F; Tozzi, Federica; Galwey, Nicholas W; Antoniades, Athos; Mooser, Vincent; Preisig, Martin; Vollenweider, Peter; Waterworth, Dawn; Waeber, Gerard; Johnson, Michael R; Muglia, Pierandrea; Balding, David J

    2010-07-09

    Neuroticism is a moderately heritable personality trait considered to be a risk factor for developing major depression, anxiety disorders and dementia. We performed a genome-wide association study in 2,235 participants drawn from a population-based study of neuroticism, making this the largest association study for neuroticism to date. Neuroticism was measured by the Eysenck Personality Questionnaire. After Quality Control, we analysed 430,000 autosomal SNPs together with an additional 1.2 million SNPs imputed with high quality from the Hap Map CEU samples. We found a very small effect of population stratification, corrected using one principal component, and some cryptic kinship that required no correction. NKAIN2 showed suggestive evidence of association with neuroticism as a main effect (p neuroticism. Our study was powered to detect almost all SNPs explaining at least 2% of heritability, and so our results effectively exclude the existence of loci having a major effect on neuroticism.

  9. Burning mouth syndrome: a retrospective study of 140 cases in a sample of Catalan population.

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    Palacios-Sánchez, Maria F; Jordana-Comín, Xavier; García-Sívoli, Carlos E

    2005-01-01

    The results of analyzing etiologic and clinical factors, and their connection with the burning mouth syndrome (BMS) in a sample of Catalan (Barcelona, Spain) population are presented in this work. The purpose of this study is to establish connections between BMS and the following variables: age, sex, overt depression, masked depression, cancerophobia, dry mouth, foreign body sensation in the mouth, and burning. 140 clinical cases of patients diagnosed with the disease and 140 cases of control patients are studied here. The data were statistically analyzed to study connections as well as the disease and variables frequency. The obtained results will help understanding possible connections of the studied etiologic and clinical factors with the disease, as well as the course of BMS, and its consequences in the Catalan population.

  10. Brazilian Cerrado Soil Actinobacteria Ecology

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    Monique Suela Silva

    2013-01-01

    Full Text Available A total of 2152 Actinobacteria strains were isolated from native Cerrado (Brazilian Savannah soils located in Passos, Luminárias, and Arcos municipalities (Minas Gerais State, Brazil. The soils were characterised for chemical and microbiological analysis. The microbial analysis led to the identification of nine genera (Streptomyces, Arthrobacter, Rhodococcus, Amycolatopsis, Microbacterium, Frankia, Leifsonia, Nakamurella, and Kitasatospora and 92 distinct species in both seasons studied (rainy and dry. The rainy season produced a high microbial population of all the aforementioned genera. The pH values of the soil samples from the Passos, Luminárias, and Arcos regions varied from 4.1 to 5.5. There were no significant differences in the concentrations of phosphorus, magnesium, and organic matter in the soils among the studied areas. Samples from the Arcos area contained large amounts of aluminium in the rainy season and both hydrogen and aluminium in the rainy and dry seasons. The Actinobacteria population seemed to be unaffected by the high levels of aluminium in the soil. Studies are being conducted to produce bioactive compounds from Actinobacteria fermentations on different substrates. The present data suggest that the number and diversity of Actinobacteria spp. in tropical soils represent a vast unexplored resource for the biotechnology of bioactives production.

  11. Parallel tagged next-generation sequencing on pooled samples - a new approach for population genetics in ecology and conservation.

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    Zavodna, Monika; Grueber, Catherine E; Gemmell, Neil J

    2013-01-01

    Next-generation sequencing (NGS) on pooled samples has already been broadly applied in human medical diagnostics and plant and animal breeding. However, thus far it has been only sparingly employed in ecology and conservation, where it may serve as a useful diagnostic tool for rapid assessment of species genetic diversity and structure at the population level. Here we undertake a comprehensive evaluation of the accuracy, practicality and limitations of parallel tagged amplicon NGS on pooled population samples for estimating species population diversity and structure. We obtained 16S and Cyt b data from 20 populations of Leiopelma hochstetteri, a frog species of conservation concern in New Zealand, using two approaches - parallel tagged NGS on pooled population samples and individual Sanger sequenced samples. Data from each approach were then used to estimate two standard population genetic parameters, nucleotide diversity (π) and population differentiation (FST), that enable population genetic inference in a species conservation context. We found a positive correlation between our two approaches for population genetic estimates, showing that the pooled population NGS approach is a reliable, rapid and appropriate method for population genetic inference in an ecological and conservation context. Our experimental design also allowed us to identify both the strengths and weaknesses of the pooled population NGS approach and outline some guidelines and suggestions that might be considered when planning future projects.

  12. Cognitive impairment in a Brazilian sample of patients with bipolar disorder Prejuízo cognitivo em uma amostra brasileira de pacientes com transtorno do humor bipolar

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    Júlia J Schneider

    2008-09-01

    Full Text Available OBJECTIVE: Persistent neurocognitive deficits have been described in bipolar mood disorder. As far as we are aware, no study have examined whether the cognitive impairment is presented in the same way in a Brazilian sample. METHOD: Cognitive function of 66 patients with bipolar disorder (32 with depressive symptoms and 34 euthymic and 28 healthy subjects was examined using a complete cognitive battery. RESULTS: Patients with bipolar disorder presented a significantly poorer performance in eight of the 12 subtests when compared to healthy subjects. There was no significant difference between the subgroups of patients. These patients showed impairment in both verbal and non-verbal cognitive function. CONCLUSION: Cognitive impairment was found in both groups of patients with bipolar disorder. The findings described here suggest an overall impairment of cognitive function, independent of mood symptoms. This is in line with data showing that cognitive deficits may be a persistent characteristic of bipolar disorder.OBJETIVO: Déficits neurocognitivos persistentes têm sido descritos no transtorno do humor bipolar; entretanto, não há estudos em amostras brasileiras para avaliar se o prejuízo se apresenta da mesma forma. MÉTODO: Foi realizada uma avaliação cognitiva em 66 pacientes bipolares (32 com sintomas depressivos e 34 eutímicos e 28 controles, utilizando-se uma bateria cognitiva completa. RESULTADOS: Em oito dos 12 subtestes avaliados os pacientes apresentaram desempenho significativamente inferior em relação aos controles. Não houve diferença significativa entre os grupos de pacientes. Foram encontrados prejuízos cognitivos tanto na área verbal como na área não verbal da cognição. CONCLUSÃO: Foi observada uma performance inferior em ambos os grupos de pacientes com transtorno bipolar. As dificuldades cognitivas encontradas apontam para um prejuízo global no funcionamento cognitivo, independente da presença de sintomas

  13. Counting Cats: Spatially Explicit Population Estimates of Cheetah (Acinonyx jubatus) Using Unstructured Sampling Data.

    Science.gov (United States)

    Broekhuis, Femke; Gopalaswamy, Arjun M

    2016-01-01

    Many ecological theories and species conservation programmes rely on accurate estimates of population density. Accurate density estimation, especially for species facing rapid declines, requires the application of rigorous field and analytical methods. However, obtaining accurate density estimates of carnivores can be challenging as carnivores naturally exist at relatively low densities and are often elusive and wide-ranging. In this study, we employ an unstructured spatial sampling field design along with a Bayesian sex-specific spatially explicit capture-recapture (SECR) analysis, to provide the first rigorous population density estimates of cheetahs (Acinonyx jubatus) in the Maasai Mara, Kenya. We estimate adult cheetah density to be between 1.28 ± 0.315 and 1.34 ± 0.337 individuals/100km2 across four candidate models specified in our analysis. Our spatially explicit approach revealed 'hotspots' of cheetah density, highlighting that cheetah are distributed heterogeneously across the landscape. The SECR models incorporated a movement range parameter which indicated that male cheetah moved four times as much as females, possibly because female movement was restricted by their reproductive status and/or the spatial distribution of prey. We show that SECR can be used for spatially unstructured data to successfully characterise the spatial distribution of a low density species and also estimate population density when sample size is small. Our sampling and modelling framework will help determine spatial and temporal variation in cheetah densities, providing a foundation for their conservation and management. Based on our results we encourage other researchers to adopt a similar approach in estimating densities of individually recognisable species.

  14. Counting Cats: Spatially Explicit Population Estimates of Cheetah (Acinonyx jubatus Using Unstructured Sampling Data.

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    Femke Broekhuis

    Full Text Available Many ecological theories and species conservation programmes rely on accurate estimates of population density. Accurate density estimation, especially for species facing rapid declines, requires the application of rigorous field and analytical methods. However, obtaining accurate density estimates of carnivores can be challenging as carnivores naturally exist at relatively low densities and are often elusive and wide-ranging. In this study, we employ an unstructured spatial sampling field design along with a Bayesian sex-specific spatially explicit capture-recapture (SECR analysis, to provide the first rigorous population density estimates of cheetahs (Acinonyx jubatus in the Maasai Mara, Kenya. We estimate adult cheetah density to be between 1.28 ± 0.315 and 1.34 ± 0.337 individuals/100km2 across four candidate models specified in our analysis. Our spatially explicit approach revealed 'hotspots' of cheetah density, highlighting that cheetah are distributed heterogeneously across the landscape. The SECR models incorporated a movement range parameter which indicated that male cheetah moved four times as much as females, possibly because female movement was restricted by their reproductive status and/or the spatial distribution of prey. We show that SECR can be used for spatially unstructured data to successfully characterise the spatial distribution of a low density species and also estimate population density when sample size is small. Our sampling and modelling framework will help determine spatial and temporal variation in cheetah densities, providing a foundation for their conservation and management. Based on our results we encourage other researchers to adopt a similar approach in estimating densities of individually recognisable species.

  15. Error baseline rates of five sample preparation methods used to characterize RNA virus populations

    Science.gov (United States)

    Kugelman, Jeffrey R.; Wiley, Michael R.; Nagle, Elyse R.; Reyes, Daniel; Pfeffer, Brad P.; Kuhn, Jens H.; Sanchez-Lockhart, Mariano; Palacios, Gustavo F.

    2017-01-01

    Individual RNA viruses typically occur as populations of genomes that differ slightly from each other due to mutations introduced by the error-prone viral polymerase. Understanding the variability of RNA virus genome populations is critical for understanding virus evolution because individual mutant genomes may gain evolutionary selective advantages and give rise to dominant subpopulations, possibly even leading to the emergence of viruses resistant to medical countermeasures. Reverse transcription of virus genome populations followed by next-generation sequencing is the only available method to characterize variation for RNA viruses. However, both steps may lead to the introduction of artificial mutations, thereby skewing the data. To better understand how such errors are introduced during sample preparation, we determined and compared error baseline rates of five different sample preparation methods by analyzing in vitro transcribed Ebola virus RNA from an artificial plasmid-based system. These methods included: shotgun sequencing from plasmid DNA or in vitro transcribed RNA as a basic “no amplification” method, amplicon sequencing from the plasmid DNA or in vitro transcribed RNA as a “targeted” amplification method, sequence-independent single-primer amplification (SISPA) as a “random” amplification method, rolling circle reverse transcription sequencing (CirSeq) as an advanced “no amplification” method, and Illumina TruSeq RNA Access as a “targeted” enrichment method. The measured error frequencies indicate that RNA Access offers the best tradeoff between sensitivity and sample preparation error (1.4−5) of all compared methods. PMID:28182717

  16. Validation of the SCOFF Questionnaire for Eating Disorders in a Multiethnic General Population Sample

    OpenAIRE

    Solmi, Francesca; Hatch, Stephani L.; Hotopf, Matthew; Treasure, Janet; Micali, Nadia

    2014-01-01

    Introduction: This study aimed to validate the SCOFF, an eating disorders (ED) screening questionnaire, in a multiethnic general population sample of adults. Method: A two-stage design was employed using the South East London Community Health Study phases I and II data. A total of 1,669 participants were screened using the SCOFF in SELCoHI, and 145 were administrated an ED clinical interview in SELCoHII. We explored the diagnostic validity of the questionnaire restricting to the 145 individua...

  17. The DRPLA CAG repeats in an Italian population sample: evaluation of the polymorphism for forensic applications.

    Science.gov (United States)

    Pelotti, S; Mantovani, V; Esposti, P D; D'Apote, L; Bragliani, M; Maiolini, E; Abbondanza, A; Pappalardo, G

    1998-03-01

    The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.

  18. Population Structure of Lutjanus purpureus (Lutjanidae - Perciformes on the Brazilian coast: further existence evidence of a single species of red snapper in the western Atlantic

    Directory of Open Access Journals (Sweden)

    Grazielle Gomes

    2012-12-01

    Full Text Available The present study focus on the mitochondrial control region to investigate phylogeographic patterns and population structure in Lutjanus purpureus, and to evaluate the genetic similarity between L. purpureus and L. campechanus. For the initial analysis, 810 base pairs sequence from control region were obtained from 239 specimens of L. purpureus collected from four localities off the Brazilian coast. The results revealed the presence of a single panmictic population characterized by high values of genetic diversity. The 299 base pairs hypervariable portion were used for the combined analysis of L. purpureus and L. campechanus, being 275 haplotypes identified in the 414 specimens. Phylogenetic tree and haplotype network did not indicate phylogeographic substructuring between the two species, but rather an intense intermingling of individuals. Considering their marked morphological similarity, the molecular data presented here indicate that only one species of red snapper exists in the western Atlantic.Para o presente estudo utilizou-se a região controle mitocondrial para investigar os padrões filogeográficos e a estrutura populacional de Lutjanus purpureus e para avaliar a similaridade genética entre L. purpureus e L. campechanus. Para a análise inicial foram obtidas sequências de 810 pares de bases (pb da região controle para 239 espécimes de L. purpureus de quatro localidades da costa brasileira. Os resultados mostraram a presença de uma população panmítica caracterizada por altos valores de diversidade genética. Utilizou-se um segmento de 299 pb da porção hipervariável para comparar L. purpureus e L. campechanus. Dos 414 indivíduos analisados foram identificados 275 haplótipos. A árvore filogenética e a rede de haplótipos não mostraram subestruturação filogeográfica entre as duas espécies, com intensa mistura de indivíduos. Considerando a grande similaridade morfológica, os dados moleculares apresentados, aqui

  19. Estudo audiológico de uma população idosa brasileira Audiological study of an elderly brazilian population

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    Luís Cláudio do Carmo

    2008-06-01

    Full Text Available A população idosa brasileira cresce e representa 8,6% do total populacional. Fatores ambientais, hábitos de vida, sexo e fatores genéticos interferem na evolução da presbiacusia que reduz a qualidade de vida. OBJETIVO: Investigar queixas audiológicas e vestibulares em idosos, executar audiometria tonal, verificar se há diferenças entre os sexos. FORMA DE ESTUDO: Clínico prospectivo de corte transversal. MATERIAL E MÉTODO: 320 pacientes idosos (160 homens e 160 mulheres foram submetidos a anamnese audiológica e audiometria tonal. Análise estatística dos resultados pelos testes ANOVA, Mann-Whitney e Qui-Quadrado. RESULTADO: As queixas audiológicas e vestibulares (perda auditiva, tinnitus, plenitude auricular, tontura foram similares entre os sexos (exceção, a tontura: pThe Brazilian elderly population is growing, and already represents 8,6% of our total population. Environmental factors, lifestyle, gender and genetics impact the development of presbycusis, which reduces quality of life. AIM: investigate audiologic and vestibular complaints in the elderly; perform tonal audiometry and check to see if there are differences between genders. STUDY: Cross-sectional clinical prospective study. MATERIALS AND METHODS: 320 elderly patients (160 men and 160 women were submitted to audiologic interview and tonal audiometry. The results were statistically analyzed by the following methods: ANOVA, Mann-Whitney and Chi-Squared. RESULTS: audiologic and vestibular complaints (hearing loss, tinnitus, ear fullness, dizziness were similar between the genders (except for dizziness: p<0,05; tonal audiometry showed a significant difference, with hearing loss in the high frequencies among men; and among women the curves were descending and flat. These results were statistically significant (P<0,001. CONCLUSION: our results lead us to conclude that, when the genders are compared, hearing loss in the elderly has similar symptoms; however, there are

  20. Efficient estimation of abundance for patchily distributed populations via two-phase, adaptive sampling.

    Science.gov (United States)

    Conroy, M.J.; Runge, J.P.; Barker, R.J.; Schofield, M.R.; Fonnesbeck, C.J.

    2008-01-01

    Many organisms are patchily distributed, with some patches occupied at high density, others at lower densities, and others not occupied. Estimation of overall abundance can be difficult and is inefficient via intensive approaches such as capture-mark-recapture (CMR) or distance sampling. We propose a two-phase sampling scheme and model in a Bayesian framework to estimate abundance for patchily distributed populations. In the first phase, occupancy is estimated by binomial detection samples taken on all selected sites, where selection may be of all sites available, or a random sample of sites. Detection can be by visual surveys, detection of sign, physical captures, or other approach. At the second phase, if a detection threshold is achieved, CMR or other intensive sampling is conducted via standard procedures (grids or webs) to estimate abundance. Detection and CMR data are then used in a joint likelihood to model probability of detection in the occupancy sample via an abundance-detection model. CMR modeling is used to estimate abundance for the abundance-detection relationship, which in turn is used to predict abundance at the remaining sites, where only detection data are collected. We present a full Bayesian modeling treatment of this problem, in which posterior inference on abundance and other parameters (detection, capture probability) is obtained under a variety of assumptions about spatial and individual sources of heterogeneity. We apply the approach to abundance estimation for two species of voles (Microtus spp.) in Montana, USA. We also use a simulation study to evaluate the frequentist properties of our procedure given known patterns in abundance and detection among sites as well as design criteria. For most population characteristics and designs considered, bias and mean-square error (MSE) were low, and coverage of true parameter values by Bayesian credibility intervals was near nominal. Our two-phase, adaptive approach allows efficient estimation of

  1. Cross-cultural analysis of the motor development of Brazilian, Greek and Canadian infants assessed with the Alberta Infant Motor Scale

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    Raquel Saccani

    2013-09-01

    Full Text Available OBJECTIVE: To compare the motor development of infants from three population samples (Brazil, Canada and Greece, to investigate differences in the percentile curves of motor development in these samples, and to investigate the prevalence of motor delays in Brazilian children. METHODS: Observational, descriptive and cross-sectional study with 795 Brazilian infants from zero to 18 months of age, assessed by the Alberta Infant Motor Scale (AIMS at day care centers, nurseries, basic health units and at home. The Brazilian infants' motor scores were compared to the results of two population samples from Greece (424 infants and Canada (2,400 infants. Descriptive statistics was used, with one-sample t-test and binomial tests, being significant p≤0.05. RESULTS: 65.4% of Brazilian children showed typical motor development, although with lower mean scores. In the beginning of the second year of life, the differences in the motor development among Brazilian, Canadian and Greek infants were milder; at 15 months of age, the motor development became similar in the three groups. A non-linear motor development trend was observed. CONCLUSIONS: The lowest motor percentiles of the Brazilian sample emphasized the need for national norms in order to correctly categorize the infant motor development. The different ways of motor development may be a consequence of cultural differences in infant care.

  2. Cross-cultural analysis of the motor development of Brazilian, Greek and Canadian infants assessed with the Alberta Infant Motor Scale

    Science.gov (United States)

    Saccani, Raquel; Valentini, Nadia Cristina

    2013-01-01

    OBJECTIVE: To compare the motor development of infants from three population samples (Brazil, Canada and Greece), to investigate differences in the percentile curves of motor development in these samples, and to investigate the prevalence of motor delays in Brazilian children. METHODS: Observational, descriptive and cross-sectional study with 795 Brazilian infants from zero to 18 months of age, assessed by the Alberta Infant Motor Scale (AIMS) at day care centers, nurseries, basic health units and at home. The Brazilian infants' motor scores were compared to the results of two population samples from Greece (424 infants) and Canada (2,400 infants). Descriptive statistics was used, with one-sample t-test and binomial tests, being significant p≤0.05. RESULTS: 65.4% of Brazilian children showed typical motor development, although with lower mean scores. In the beginning of the second year of life, the differences in the motor development among Brazilian, Canadian and Greek infants were milder; at 15 months of age, the motor development became similar in the three groups. A non-linear motor development trend was observed. CONCLUSIONS: The lowest motor percentiles of the Brazilian sample emphasized the need for national norms in order to correctly categorize the infant motor development. The different ways of motor development may be a consequence of cultural differences in infant care. PMID:24142318

  3. [The biomonitoring of toxic substances in biological samples of general population].

    Science.gov (United States)

    Ibarluzea, Jesús; Aurrekoetxea, Juan José; Porta, Miquel; Sunyer, Jordi; Ballester, Ferran

    2016-11-01

    Many of the world's most developed countries have adopted biomonitoring of toxic substances in order to ascertain their levels in biological samples. These substances get into the body through different environmental exposures. Monitoring toxic substances in biological samples should allow us to ascertain their levels in vulnerable groups, assess their evolution over time, make comparisons with levels observed in other countries, identify groups at risk or with high toxic levels and promote research. The main objective of biomonitoring is to act as a policy design tool to facilitate the implementation of particular measures in various sectors: health, environmental, agricultural and livestock or food industry sectors. In Spain, information on levels of toxic substances of environmental origin is provided by specific studies on health effects from environmental sources, such as the INMA project (INfancia y Medio Ambiente [childhood and environment]). In addition, biomonitoring projects have been implemented in Catalonia and the Canary Islands, together with a national biomonitoring programme in the adult working population. However, further progress is needed to develop a system that covers the general population as well as subgroups at risk, which relies on the collaboration of the involved authorities and the participation of professionals from different sectors and citizen organisations interested in the relationship between health and the environment.

  4. Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae populations of the tropical West Atlantic

    Directory of Open Access Journals (Sweden)

    Anderson V. Vasconcellos

    2008-01-01

    Full Text Available Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p < 0.0001. Analyses of mitochondrial DNA sequences (663 bp of the control region did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p < 0.05, but this may have resulted from the small number of samples analysed for that population. Sequence variation of Belize samples departed from neutral expectations (Fu's FS = -8.88; p < 0.001. A mismatch distribution analysis points to an ancient population expansion in that area. We conclude that the genetic data do not allow the rejection of the null hypothesis of panmixia for Brazilian yellowtail snapper populations which should be treated as a single genetic stock, with a latitudinal gradient on their morphology which probably results from phenotypic plasticity. On the other hand, there is a severe restriction to gene flow between O. chrysurus populations from the Caribbean and from the southwestern Atlantic.

  5. Genetic structure and natal origins of immature hawksbill turtles (Eretmochelys imbricata in Brazilian waters.

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    Maira C Proietti

    Full Text Available Understanding the connections between sea turtle populations is fundamental for their effective conservation. Brazil hosts important hawksbill feeding areas, but few studies have focused on how they connect with nesting populations in the Atlantic. Here, we (1 characterized mitochondrial DNA control region haplotypes of immature hawksbills feeding along the coast of Brazil (five areas ranging from equatorial to temperate latitudes, 157 skin samples, (2 analyzed genetic structure among Atlantic hawksbill feeding populations, and (3 inferred natal origins of hawksbills in Brazilian waters using genetic, oceanographic, and population size information. We report ten haplotypes for the sampled Brazilian sites, most of which were previously observed at other Atlantic feeding grounds and rookeries. Genetic profiles of Brazilian feeding areas were significantly different from those in other regions (Caribbean and Africa, and a significant structure was observed between Brazilian feeding grounds grouped into areas influenced by the South Equatorial/North Brazil Current and those influenced by the Brazil Current. Our genetic analysis estimates that the studied Brazilian feeding aggregations are mostly composed of animals originating from the domestic rookeries Bahia and Pipa, but some contributions from African and Caribbean rookeries were also observed. Oceanographic data corroborated the local origins, but showed higher connection with West Africa and none with the Caribbean. High correlation was observed between origins estimated through genetics/rookery size and oceanographic/rookery size data, demonstrating that ocean currents and population sizes influence haplotype distribution of Brazil's hawksbill populations. The information presented here highlights the importance of national conservation strategies and international cooperation for the recovery of endangered hawksbill turtle populations.

  6. Characterization of mitochondrial haplogroups in a large population-based sample from the United States.

    Science.gov (United States)

    Mitchell, Sabrina L; Goodloe, Robert; Brown-Gentry, Kristin; Pendergrass, Sarah A; Murdock, Deborah G; Crawford, Dana C

    2014-07-01

    Mitochondrial DNA (mtDNA) haplogroups are valuable for investigations in forensic science, molecular anthropology, and human genetics. In this study, we developed a custom panel of 61 mtDNA markers for high-throughput classification of European, African, and Native American/Asian mitochondrial haplogroup lineages. Using these mtDNA markers, we constructed a mitochondrial haplogroup classification tree and classified 18,832 participants from the National Health and Nutrition Examination Surveys (NHANES). To our knowledge, this is the largest study to date characterizing mitochondrial haplogroups in a population-based sample from the United States, and the first study characterizing mitochondrial haplogroup distributions in self-identified Mexican Americans separately from Hispanic Americans of other descent. We observed clear differences in the distribution of maternal genetic ancestry consistent with proposed admixture models for these subpopulations, underscoring the genetic heterogeneity of the United States Hispanic population. The mitochondrial haplogroup distributions in the other self-identified racial/ethnic groups within NHANES were largely comparable to previous studies. Mitochondrial haplogroup classification was highly concordant with self-identified race/ethnicity (SIRE) in non-Hispanic whites (94.8 %), but was considerably lower in admixed populations including non-Hispanic blacks (88.3 %), Mexican Americans (81.8 %), and other Hispanics (61.6 %), suggesting SIRE does not accurately reflect maternal genetic ancestry, particularly in populations with greater proportions of admixture. Thus, it is important to consider inconsistencies between SIRE and genetic ancestry when performing genetic association studies. The mitochondrial haplogroup data that we have generated, coupled with the epidemiologic variables in NHANES, is a valuable resource for future studies investigating the contribution of mtDNA variation to human health and disease.

  7. Sampling strategy for wild soybean (Glycine soja) populations based on their genetic diversity and fine-scale spatial genetic structure

    Institute of Scientific and Technical Information of China (English)

    ZHU Weiyue; ZHOU Taoying; ZHONG Ming; LU Baorong

    2007-01-01

    A total of 892 individuals sampled from a wild soybean population in a natural reserve near the Yellow River estuary located in Kenli of Shandong Province (China) were investigated.Seventeen SSR (simple sequence repeat) primer pairs from cultivated soybeans were used to estimate the genetic diversity of the population and its variation pattern versus changes of the sample size (sub-samples),in addition to investigating the fine-scale spatial genetic structure within the population.The results showed relatively high genetic diversity of the population with the mean value of allele number (A) being 2.88,expected heterozygosity (He) 0.431,Shannon diversity index (/) 0.699,and percentage of polymorphic loci (P) 100%.Sub-samples of different sizes (ten groups) were randomly drawn from the population and their genetic diversity was calculated by computer simulation.The regression model of the four diversity indexes with the change of sample sizes was computed.As a result,27-52 individuals can reach 95% of total genetic variability of the population.Spatial autocorrelation analysis revealed that the genetic patch size of this wild soybean population is about 18 m.The study provided a scientific basis for the sampling strategy of wild soybean populations.

  8. Reliable Quantification of the Potential for Equations Based on Spot Urine Samples to Estimate Population Salt Intake

    DEFF Research Database (Denmark)

    Huang, Liping; Crino, Michelle; Wu, Jason Hy

    2016-01-01

    BACKGROUND: Methods based on spot urine samples (a single sample at one time-point) have been identified as a possible alternative approach to 24-hour urine samples for determining mean population salt intake. OBJECTIVE: The aim of this study is to identify a reliable method for estimating mean p...

  9. The association between Internet addiction and personality disorders in a general population-based sample

    Science.gov (United States)

    Zadra, Sina; Bischof, Gallus; Besser, Bettina; Bischof, Anja; Meyer, Christian; John, Ulrich; Rumpf, Hans-Jürgen

    2016-01-01

    Background and aims Data on Internet addiction (IA) and its association with personality disorder are rare. Previous studies are largely restricted to clinical samples and insufficient measurement of IA. Methods Cross-sectional analysis data are based on a German sub-sample (n = 168; 86 males; 71 meeting criteria for IA) with increased levels of excessive Internet use derived from a general population sample (n = 15,023). IA was assessed with a comprehensive standardized interview using the structure of the Composite International Diagnostic Interview and the criteria of Internet Gaming Disorder as suggested in DSM-5. Impulsivity, attention deficit hyperactivity disorder, and self-esteem were assessed with the widely used questionnaires. Results Participants with IA showed higher frequencies of personality disorders (29.6%) compared to those without IA (9.3%; p disorders were more prevalent than among non-addicted males. Compared to participants who had IA only, lower rates of remission of IA were found among participants with IA and additional cluster B personality disorder. Personality disorders were significantly associated with IA in multivariate analysis. Discussion and conclusion: Comorbidity of IA and personality disorders must be considered in prevention and treatment. PMID:28005417

  10. Social orientation, sexual role, and moral judgment: a comparison of two brazilian and one norwegian sample / Orientação social, papel sexual e julgamento moral: uma comparação entre duas amostras brasileiras e uma norueguesa

    Directory of Open Access Journals (Sweden)

    Angela Biaggio

    2005-01-01

    Full Text Available Thirty female and 30 male university students each from Joao Pessoa and Porto Alegre were compared to a comparable Norwegian sample of 60 female and 60 male students. Except for a suggestion of differences in women's cultural orientation, comparisons on Gibbs' test of justice morality, the ECI test for ethic of care, Bem's sex role inventory, and Triandis' test for cultural orientations showed that all differences were between the Norwegian sample and the Brazilian samples as a unit. Brazilians showed a differentiation of sex roles, which was not shown in Norwegians, and higher scores on the collectivism cultural orientation. Norwegians showed higher scores ECI, which might be because of a culture bias in the test. No difference was shown for individualism cultural orientation, and on Gibbs' test. Men scored higher on the total individualism measure, and women on vertical collectivism. JP women scored as more hedonistic and individual than the PA women, who scores as more traditional than the JP women.

  11. Importance of sampling design and analysis in animal population studies: a comment on Sergio et al

    Science.gov (United States)

    Kery, M.; Royle, J. Andrew; Schmid, Hans

    2008-01-01

    1. The use of predators as indicators and umbrellas in conservation has been criticized. In the Trentino region, Sergio et al. (2006; hereafter SEA) counted almost twice as many bird species in quadrats located in raptor territories than in controls. However, SEA detected astonishingly few species. We used contemporary Swiss Breeding Bird Survey data from an adjacent region and a novel statistical model that corrects for overlooked species to estimate the expected number of bird species per quadrat in that region. 2. There are two anomalies in SEA which render their results ambiguous. First, SEA detected on average only 6.8 species, whereas a value of 32 might be expected. Hence, they probably overlooked almost 80% of all species. Secondly, the precision of their mean species counts was greater in two-thirds of cases than in the unlikely case that all quadrats harboured exactly the same number of equally detectable species. This suggests that they detected consistently only a biased, unrepresentative subset of species. 3. Conceptually, expected species counts are the product of true species number and species detectability p. Plenty of factors may affect p, including date, hour, observer, previous knowledge of a site and mobbing behaviour of passerines in the presence of predators. Such differences in p between raptor and control quadrats could have easily created the observed effects. Without a method that corrects for such biases, or without quantitative evidence that species detectability was indeed similar between raptor and control quadrats, the meaning of SEA's counts is hard to evaluate. Therefore, the evidence presented by SEA in favour of raptors as indicator species for enhanced levels of biodiversity remains inconclusive. 4. Synthesis and application. Ecologists should pay greater attention to sampling design and analysis in animal population estimation. Species richness estimation means sampling a community. Samples should be representative for the

  12. Compensating for population sampling in simulations of epidemic spread on temporal contact networks

    CERN Document Server

    Génois, Mathieu; Cattuto, Ciro; Barrat, Alain

    2015-01-01

    Data describing human interactions often suffer from incomplete sampling of the underlying population. As a consequence, the study of contagion processes using data-driven models can lead to a severe underestimation of the epidemic risk. Here we present a systematic method to correct this bias and obtain an accurate estimation of the risk in the context of epidemic models informed by high-resolution time-resolved contact data. We consider several such data sets collected in various contexts and perform controlled resampling experiments. We show that the statistical information contained in the resampled data allows us to build surrogate versions of the unknown contacts and that simulations of epidemic processes using these surrogate data sets yield good estimates of the outcome of simulations performed using the complete data set. We discuss limitations and potential improvements of our method.

  13. A three-sample multiple-recapture approach to census population estimation with heterogeneous catchability.

    Science.gov (United States)

    Derroch, J N; Fienberg, S E; Glonek, G F; Junker, B W

    1993-09-01

    "A central assumption in the standard capture-recapture approach to the estimation of the size of a closed population is the homogeneity of the 'capture' probabilities. In this article we develop an approach that allows for varying susceptibility to capture through individual parameters using a variant of the Rasch model from psychological measurement situations. Our approach requires an additional recapture. In the context of census undercount estimation, this requirement amounts to the use of a second independent sample or alternative data source to be matched with census and Post-Enumeration Survey (PES) data.... We illustrate [our] models and their estimation using data from a 1988 dress-rehearsal study for the 1990 census conducted by the U.S. Bureau of the Census, which explored the use of administrative data as a supplement to the PES. The article includes a discussion of extensions and related models."

  14. Beck Anxiety Inventory: psychometric characteristics in a sample from the clinical Spanish population.

    Science.gov (United States)

    Vázquez Morejón, Antonio J; Vázquez-Morejón Jiménez, Raquel; Zanin, Gloria Bellido

    2014-10-28

    Even though the Beck Anxiety Inventory (BAI) is one of the most popular instruments to assess anxiety today, only limited data is available about its psychometric characteristics and normative values in clinical Spanish populations. A study was conducted to test the psychometric characteristics of a Spanish adaptation of the Beck Anxiety Inventory (BAI) in a sample of 918 outpatients being treated at a community mental health center in Spain. Results confirmed the adaptation's high internal consistency (∝ = .91), substantial test-retest reliability at 8-10 weeks (r = .84, p Anxiety (r = .86, p Phobic Anxiety (r = .63, p < .01) dimensions of the SCL-90-R, and with the Anxious Thoughts Inventory (r = .57, p < .01). Gender differences in BAI scores did occur, so normative values appear separately for each gender.

  15. [Associations between body dysmorphic symptoms, body image and self-consciousness in a representative population sample].

    Science.gov (United States)

    Daig, Isolde; Burkert, Silke; Albani, Cornelia; Martin, Alexandra; Brähler, Elmar

    2008-01-01

    This study investigates the association between body image and body dysmorphic symptoms in the population by taking different kinds of self-consciousness into account. Body dysmorphic symptoms are characterized by a distressing and impairing preoccupation with an imagined or slight defect in appearance. A representative sample of 1621 persons in Germany was examined with screening instruments for body image, body dysmorphic symptoms and self-consciousness. Women reported more body dysmorphic symptoms and higher self-consciousness than men. People with body dysmorphic symptoms reported a more negative body image and higher self-consciousness. Different aspects of self-consciousness moderated the association between body dysmorphic symptoms and negative body image. Results were discussed towards hypotheses of affect modulation and depression tendency of patients with body dysmorphic symptoms.

  16. Descriptive epidemiology of stigma against depression in a general population sample in Alberta

    Directory of Open Access Journals (Sweden)

    Wang JianLi

    2010-04-01

    Full Text Available Abstract Background Mental health illnesses, such as depression, are responsible for a growing disease burden worldwide. Unfortunately, effective treatment is often impeded by stigmatizing attitudes of other individuals, which have been found to lead to a number of negative consequences including reduced help-seeking behavior and increased social distance. Despite the high prevalence of depression in Canada, little research has been conducted to examine stigma against depression in the Canadian general population. Such information is crucial to understanding the current state of stigmatizing attitudes in the Canadian communities, and framing future stigma reduction initiatives. The objectives of this study were to estimate the percentages of various stigmatizing attitudes toward depression in a general population sample and to compare the percentages by demographics and socioeconomic characteristics. Methods We conducted a cross-sectional telephone survey in Alberta, Canada, between February and June 2006. Random digit dialing was used to recruit participants who were aged 18-74 years old (n = 3047. Participants were presented a case vignette describing a depressed individual, and responded to a 9-item Personal Stigma questionnaire. The percentages of stigmatizing attitudes were estimated and compared by demographic and socioeconomic variables. Results Among the participants, 45.9% endorsed that depressed individuals were unpredictable and 21.9% held the view that people with depression were dangerous. Significant differences in stigmatizing attitudes were found by gender, age, education, and immigration status. A greater proportion of men than women held stigmatizing views on each stigma item. No consistent trend emerged by age in stigma against depression. Participants with higher levels of education reported less stigmatizing attitudes than those with less education. Participants who were not born in Canada were more likely to hold

  17. Population genetics inference for longitudinally-sampled mutants under strong selection.

    Science.gov (United States)

    Lacerda, Miguel; Seoighe, Cathal

    2014-11-01

    Longitudinal allele frequency data are becoming increasingly prevalent. Such samples permit statistical inference of the population genetics parameters that influence the fate of mutant variants. To infer these parameters by maximum likelihood, the mutant frequency is often assumed to evolve according to the Wright-Fisher model. For computational reasons, this discrete model is commonly approximated by a diffusion process that requires the assumption that the forces of natural selection and mutation are weak. This assumption is not always appropriate. For example, mutations that impart drug resistance in pathogens may evolve under strong selective pressure. Here, we present an alternative approximation to the mutant-frequency distribution that does not make any assumptions about the magnitude of selection or mutation and is much more computationally efficient than the standard diffusion approximation. Simulation studies are used to compare the performance of our method to that of the Wright-Fisher and Gaussian diffusion approximations. For large populations, our method is found to provide a much better approximation to the mutant-frequency distribution when selection is strong, while all three methods perform comparably when selection is weak. Importantly, maximum-likelihood estimates of the selection coefficient are severely attenuated when selection is strong under the two diffusion models, but not when our method is used. This is further demonstrated with an application to mutant-frequency data from an experimental study of bacteriophage evolution. We therefore recommend our method for estimating the selection coefficient when the effective population size is too large to utilize the discrete Wright-Fisher model.

  18. Adverse childhood experiences, gender, and HIV risk behaviors: Results from a population-based sample

    Directory of Open Access Journals (Sweden)

    Lin Fang

    2016-12-01

    Full Text Available Recent HIV research suggested assessing adverse childhood experiences (ACEs as contributing factors of HIV risk behaviors. However, studies often focused on a single type of adverse experience and very few utilized population-based data. This population study examined the associations between ACE (individual and cumulative ACE score and HIV risk behaviors. We analyzed the 2012 Behavioral Risk Factor Surveillance Survey (BRFSS from 5 states. The sample consisted of 39,434 adults. Eight types of ACEs that included different types of child abuse and household dysfunctions before the age of 18 were measured. A cumulative score of ACEs was also computed. Logistic regression estimated of the association between ACEs and HIV risk behaviors using odds ratio (OR with 95% confidence intervals (CIs for males and females separately. We found that ACEs were positively associated with HIV risk behaviors overall, but the associations differed between males and females in a few instances. While the cumulative ACE score was associated with HIV risk behaviors in a stepwise manner, the pattern varied by gender. For males, the odds of HIV risk increased at a significant level as long as they experienced one ACE, whereas for females, the odds did not increase until they experienced three or more ACEs. Future research should further investigate the gender-specific associations between ACEs and HIV risk behaviors. As childhood adversities are prevalent among general population, and such experiences are associated with increased risk behaviors for HIV transmission, service providers can benefit from the principles of trauma-informed practice.

  19. Ethnic differences in sleep duration and morning–evening type in a population sample

    Science.gov (United States)

    Malone, Susan Kohl; Patterson, Freda; Lu, Yinghui; Lozano, Alicia; Hanlon, Alexandra

    2016-01-01

    This cross-sectional population study examined associations of sleep duration and morning–evening type with sociodemographic and cardiometabolic disease in adults participating in the UK Biobank study (N = 439 933). Multivariable Poisson regression models of sleep duration and morning–evening type with a robust error variance were generated to estimate adjusted prevalence ratios and their 95% confidence intervals. All models were adjusted for sex, race, college attendance, employment status and age. Twenty five percent of the sample reported short sleep; 27% were morning, 64% intermediate and 9% evening type. Black ethnicity emerged as most strongly associated with sleep behavior. Short sleep was twice as prevalent, and morning versus intermediate type was 1.4 times more prevalent in Black than White participants. The greater prevalence of short sleep and morning type among Blacks suggests that sleep-based approaches to improving cardiometabolic outcomes may require a more multidimensional approach that encompasses adequate sleep and circadian alignment in this population. PMID:26654569

  20. Population structure of Legionella spp. from environmental samples in Gabon, 2013.

    Science.gov (United States)

    Ehrhardt, Jonas; Alabi, Abraham S; Kuczius, Thorsten; Tsombeng, Francis Foguim; Becker, Karsten; Kremsner, Peter G; Schaumburg, Frieder; Esen, Meral

    2015-07-01

    Aquatic environments are the most important source for Legionella spp. infections such as Legionnaires' disease and Pontiac fever. The reservoirs of Legionella spp. are mostly unclear in sub-Saharan Africa. The aim of this study, conducted in 2013, was to identify geographical areas of an increased risk for exposure to Legionella spp., and to describe the population structure of Legionella spp. from different water sources in a cross-sectional study in Gabon. Fresh water samples (n = 200) were cultured on Legionella selective agar; species were confirmed by MALDI-TOF, a Legionella pneumophila specific real-time PCR and 16S RNA gene sequencing. Serogroups were identified by agglutination test. The population structure was assessed by multilocus sequence typing (MLST). Legionella spp. isolates (n = 29) were frequently found in the hospital setting particularly in hot water systems. Open water bodies (i.e. rivers, lakes) were not contaminated with Legionella spp. Isolated L. pneumophila mainly belonged to serogroups 2-14 (n = 19) and MLST sequence type ST1, ST75 (and related STs) and ST1911. In conclusion, hospitalized patients might have an increased risk to become infected with Legionella spp. in the studied areas in Gabon, particularly if they have risk factors such as comorbidities. Both broadly extended (ST1, ST75) and local lineages (ST1911) were present in our setting.

  1. A genome-wide association study of neuroticism in a population-based sample.

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    Federico C F Calboli

    Full Text Available Neuroticism is a moderately heritable personality trait considered to be a risk factor for developing major depression, anxiety disorders and dementia. We performed a genome-wide association study in 2,235 participants drawn from a population-based study of neuroticism, making this the largest association study for neuroticism to date. Neuroticism was measured by the Eysenck Personality Questionnaire. After Quality Control, we analysed 430,000 autosomal SNPs together with an additional 1.2 million SNPs imputed with high quality from the Hap Map CEU samples. We found a very small effect of population stratification, corrected using one principal component, and some cryptic kinship that required no correction. NKAIN2 showed suggestive evidence of association with neuroticism as a main effect (p < 10(-6 and GPC6 showed suggestive evidence for interaction with age (p approximately = 10(-7. We found support for one previously-reported association (PDE4D, but failed to replicate other recent reports. These results suggest common SNP variation does not strongly influence neuroticism. Our study was powered to detect almost all SNPs explaining at least 2% of heritability, and so our results effectively exclude the existence of loci having a major effect on neuroticism.

  2. A probabilistic formulation for Empirical Population Synthesis Sampling methods and tests

    CERN Document Server

    Fernandes, R C; Schmitt, H; Leao, J; Fernandes, Roberto Cid; Sodre, Laerte; Schmitt, Henrique

    2001-01-01

    We present a probabilistic formulation of the classical problem of synthesizing spectral properties of a galaxy using a base of star clusters. The problem consists of estimating the population vector x, composed by the contributions of n_star base elements to the integrated spectrum of a galaxy, and the extinction A_V, given a set of absorption line equivalent widths and continuum colors. The formalism is applied to the n_star = 12 base defined by Schmidt etal and subsequently used in several studies. The 13-D parameter space is explored with a Markov chain Monte Carlo sampling scheme based on the Metropolis algorithm, which produces a smooth and efficient mapping of the P(x,A_V) probability distribution. This version of Empirical Population Synthesis is used to investigate the ability to recover the detailed history of star-formation and chemical evolution using this spectral base. This is studied as a function of (1) the magnitude of the measurement errors and (2) the set of observables used in the synthesi...

  3. Normative data for the Montreal Cognitive Assessment in an Italian population sample.

    Science.gov (United States)

    Santangelo, Gabriella; Siciliano, Mattia; Pedone, Roberto; Vitale, Carmine; Falco, Fabrizia; Bisogno, Rossella; Siano, Pietro; Barone, Paolo; Grossi, Dario; Santangelo, Franco; Trojano, Luigi

    2015-04-01

    The Montreal Cognitive Assessment (MoCA) is a rapid screening battery, also including subtests to assess frontal functions such as set-shifting, abstraction and cognitive flexibility. MoCA seems to be useful to identify non-amnestic mild cognitive impairment (MCI) and subcortical dementia; it has high sensitivity and specificity in distinguishing MCI from mild Alzheimer's Disease. Previous studies revealed that certain items of MoCA may be culturally biased and highlighted the need for population-based norms for the MoCA. The aim of present study was to collect normative values in a sample of Italian healthy subjects. Four hundred and fifteen Italian healthy subjects (252 women and 163 men) of different ages (age range 21-95 years) and educational level (from primary to university) underwent MoCA and Mini Mental State Examination (MMSE). Multiple linear regression analysis revealed that age and education significantly influenced performance on MoCA. No significant effect of gender was found. From the derived linear equation, a correction grid for MoCA raw scores was built. Inferential cut-off score, estimated using a non-parametric technique, is 15.5 and equivalent scores were computed. Correlation analysis showed a significant but weak correlation between MoCA adjusted scores with MMSE adjusted scores (r = 0.43, p normative data for the MoCA in an Italian population useful for both clinical and research purposes.

  4. Classification of Water Masses and Targeted Sampling of Ocean Plankton Populations by an Autonomous Underwater Vehicle

    Science.gov (United States)

    Zhang, Y.; Ryan, J. P.; Bellingham, J. G.; Harvey, J.; McEwen, R.; Chavez, F.; Scholin, C.

    2011-12-01

    Autonomous underwater vehicles (AUVs) are playing an increasingly active role in oceanographic surveys due to their mobility, efficiency, and growing intelligence. The Dorado AUV is equipped with a comprehensive suite of in situ sensors and ten 1.8-liter water samplers (called "gulpers"). During an October 2010 experiment in Monterey Bay, the AUV ran our autonomous peak-capture algorithm to acquire chlorophyll/backscatter peak samples from a phytoplankton bloom, allowing biologists to successfully monitor fluctuations in harmful microalgae (Psuedo-nitzschia spp.), the toxin they produce (domoic acid), and co-occurring zooplankton (invertebrate larvae and copepods) over space and time. For further investigations of the complex marine ecosystem in northern Monterey Bay, we set a more challenging goal: when the AUV flies from an upwelling shadow region (stratified water column) through an upwelling front into newly upwelled water, can it autonomously distinguish among water columns with different vertical structures and accordingly sample plankton populations on either side of, as well as within, the upwelling front? To achieve this goal, we have developed two new algorithms, one for distinguishing upwelling water columns from stratified water columns based on the vertical homogeneity of temperature, and the other for detecting an upwelling front based on the horizontal gradient of temperature. For acquiring targeted water samples, the 10 gulpers are appropriately allocated to the two distinct water columns and the front. Lockout time intervals between triggerings are set to prevent "dense triggerings". During our June 2011 experiment, the Dorado AUV flew westward from an upwelling shadow region (stratified water column) through an upwelling front, and into an upwelling water column. Three gulpers were allocated to the stratified water column, four to the front, and the remaining three to the upwelling water column. The AUV successfully detected and acquired targeted

  5. The relation between exercise and glaucoma in a South Korean population-based sample

    Science.gov (United States)

    Lin, Shuai-Chun; Wang, Sophia Y.; Pasquale, Louis R.; Singh, Kuldev; Lin, Shan C.

    2017-01-01

    Purpose To investigate the association between exercise and glaucoma in a South Korean population-based sample. Design Population-based, cross-sectional study. Participants A total of 11,246 subjects, 40 years and older who underwent health care assessment as part of the 2008–2011 Korean National Health and Nutrition Examination Survey. Methods Variables regarding the duration (total minutes per week), frequency (days per week), and intensity of exercise (vigorous, moderate exercise and walking) as well as glaucoma prevalence were ascertained for 11,246 survey participants. Demographic, comorbidity, and health-related behavior information was obtained via interview. Multivariable logistic regression analyses were performed to determine the association between the exercise-related parameters and odds of a glaucoma diagnosis. Main outcome measure(s) Glaucoma defined by International Society for Geographical and Epidemiological Ophthalmology criteria. Results Overall, 336 (2.7%) subjects met diagnostic criteria for glaucomatous disease. After adjustment for potential confounding variables, subjects engaged in vigorous exercise 7 days per week had higher odds of having glaucoma compared with those exercising 3 days per week (Odds Ratio [OR] 3.33, 95% confidence interval [CI] 1.16–9.54). High intensity of exercise, as categorized by the guidelines of the American College of Sports Medicine (ACSM), was also associated with greater glaucoma prevalence compared with moderate intensity of exercise (OR 1.55, 95% CI 1.03–2.33). There was no association between other exercise parameters including frequency of moderate exercise, walking, muscle strength exercise, flexibility training, or total minutes of exercise per week, and the prevalence of glaucoma. In sub-analyses stratifying by gender, the association between frequency of vigorous exercise 7 days per week and glaucoma diagnosis remained significant in men (OR 6.05, 95% CI 1.67–21.94) but not in women (OR 0.96 95

  6. Morphometric analysis of population samples of soldier caste of Odontotermes obesus (Rambur (Isoptera, Termitidae, Macrotermitinae

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    Manzoor, F.

    2006-12-01

    Full Text Available In order to study morphometric variations in Odontotermes obesus (Rambur, samples from nineteen nests were statistically analyzed for mean, standard deviation, standard error, coefficient of variability and confidence interval (95% and analysis of variance (Model II ANOVA, The mean values of the different population samples were compared with the student t-test, following the Minitab version and Sokal & Rohlf (1973. In the study of external characters, measurements form a very important component, particularly for identification of species. However, the reliability of the measurements depends on the extent of variability which the structures show within and between colonies. For each individual soldier, the following nine parameters were measured: i length of head; ii width of head at mandibles; iii width of head at the posterolateral ends of antennal carinae; iv maximum width of head; v length of left mandible; vi tooth of left mandible from tip; vii length of pronotum; viii width of pronotum; ix length of postmentum; and x width of postmentum.

  7. Validation of the Rasch-based Depression Screening in a large scale German general population sample

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    Norra Christine

    2010-09-01

    Full Text Available Abstract Background The study aimed at presenting normative data for both parallel forms of the "Rasch-based Depression Screening (DESC", to examine its Rasch model conformity and convergent and divergent validity based on a representative sample of the German general population. Methods The sample was selected with the assistance of a demographic consulting company applying a face to face interview (N = 2509; mean age = 49.4, SD = 18.2; 55.8% women. Adherence to Rasch model assumptions was determined with analysis of Rasch model fit (infit and outfit, unidimensionality, local independence (principal component factor analysis of the residuals, PCFAR and differential item functioning (DIF with regard to participants' age and gender. Norm values were calculated. Convergent and divergent validity was determined through intercorrelations with the depression and anxiety subscales of the Hospital Anxiety and Depression Scale (HADS-D and HADS-A. Results Fit statistics were below critical values (rDESC-I = .61 and rDESC-II = .60, whereas correlations with HADS-A were rDESC-I = .62 and rDESC-II = .60. Conclusions This study provided further support for the psychometric quality of the DESC. Both forms of the DESC adhered to Rasch model assumptions and showed intercorrelations with HADS subscales that are in line with the literature. The presented normative data offer important advancements for the interpretation of the questionnaire scores and enhance its usefulness for clinical and research applications.

  8. Balancing Sodium and Potassium: Estimates of Intake in a New Zealand Adult Population Sample

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    Rachael McLean

    2015-10-01

    Full Text Available Dietary intakes of sodium and potassium are important determinants of blood pressure. We assessed sodium and potassium intake in a cross-sectional survey which included a random sample of New Zealand Adults aged 18 to 64 years from two New Zealand cities: Dunedin and Wellington. Participants completed a short questionnaire, had height, weight and blood pressure measured, and collected a 24 h urine sample. Mean 24 h sodium excretion was 3386 mg/day (95% CI 3221, 3551: 3865 mg/day for men and for 2934 mg/day women. Mean 24 h potassium excretion was 2738 mg/day (95% CI 2623, 2855: 3031 mg/day for men and 2436 mg/day for women. Mean sodium:potassium ratio was 1.32 (95% CI 1.26, 1.39; 1.39 for men and 1.26 for women. Sodium intake was higher among younger people, men, those with a higher BMI and higher potassium excretion. Potassium excretion was higher among older people, men and those with a higher sodium excretion. New Zealand adults have high sodium intakes and low potassium intakes compared to recommended levels. This is likely to adversely affect population blood pressure levels as well as incidence of cardiovascular disease. A comprehensive public health programme to reduce dietary sodium intake and increase intake of fruit and vegetables is warranted.

  9. Validation of the SCOFF Questionnaire for Eating Disorders in a Multiethnic General Population Sample

    Science.gov (United States)

    Solmi, Francesca; Hatch, Stephani L; Hotopf, Matthew; Treasure, Janet; Micali, Nadia

    2015-01-01

    Introduction This study aimed to validate the SCOFF, an eating disorders (ED) screening questionnaire, in a multiethnic general population sample of adults. Method A two-stage design was employed using the South East London Community Health Study phases I and II data. A total of 1,669 participants were screened using the SCOFF in SELCoHI, and 145 were administrated an ED clinical interview in SELCoHII. We explored the diagnostic validity of the questionnaire restricting to the 145 individuals with the clinical questionnaire. Results Sensitivity and specificity of the SCOFF were 53.7 and 93.5%, respectively. Conclusion The SCOFF showed good levels of specificity but low sensitivity, resulting in a high percentage of false negatives. Given the low sensitivity found in our sample the SCOFF is likely to be a suboptimal measure for the identification of ED in the community. © 2014 The Authors International Journal of Eating Disorders Published by Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:312–316) PMID:25504212

  10. Bolton tooth size ratio among qatari population sample: An odontometric study

    Science.gov (United States)

    Hashim, Hayder A; AL-Sayed, Najah; AL-Hussain, Hashim

    2017-01-01

    Objectives: To establish the overall and anterior Bolton ratio among a sample of Qatari population and to investigate whether there is a difference between males and females, as well as to compare the result obtained by Bolton. Materials and Methods: The current study consisted of 100 orthodontic study participants (50 males and 50 females) with different malocclusions and age ranging between 15 and 20 years. An electronic digital caliper was used to measure the mesiodistal tooth width of all maxillary and mandibular permanent teeth except second and third molars. The Student's t-test was used to compare tooth-size ratios between males and females and between the results of the present study and Bolton's result. Results: The anterior and overall ratio in Qatari individuals were 78.6 ± 3.4 and 91.8 ± 3.1, respectively. The tooth size ratios were slightly greater in males than that in females, however, the differences were not statistically significant (P > 0.05). There were no significant differences in the overall ratio between Qatari individuals and Bolton's results (P > 0.05), whereas statistical significant differences were observed in anterior ratio (P = 0.007). Conclusions: Within the limitation of the limitations of the present study, definite conclusion was difficult to establish. Thus, a further study with a large sample in each malocclusion group is required. PMID:28197399

  11. The Impact of Sampling Approach on Population Invariance in Automated Scoring of Essays. Research Report. ETS RR-13-18

    Science.gov (United States)

    Zhang, Mo

    2013-01-01

    Many testing programs use automated scoring to grade essays. One issue in automated essay scoring that has not been examined adequately is population invariance and its causes. The primary purpose of this study was to investigate the impact of sampling in model calibration on population invariance of automated scores. This study analyzed scores…

  12. Recruitment of representative samples for low incidence cancer populations: Do registries deliver?

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    Sanson-Fisher Rob

    2011-01-01

    Full Text Available Abstract Background Recruiting large and representative samples of adolescent and young adult (AYA cancer survivors is important for gaining accurate data regarding the prevalence of unmet needs in this population. This study aimed to describe recruitment rates for AYAs recruited through a cancer registry with particular focus on: active clinician consent protocols, reasons for clinicians not providing consent and the representativeness of the final sample. Methods Adolescents and young adults aged 14 to19 years inclusive and listed on the cancer registry from January 1 2002 to December 31 2007 were identified. An active clinician consent protocol was used whereby the registry sent a letter to AYAs primary treating clinicians requesting permission to contact the survivors. The registry then sent survivors who received their clinician's consent a letter seeking permission to forward their contact details to the research team. Consenting AYAs were sent a questionnaire which assessed their unmet needs. Results The overall consent rate for AYAs identified as eligible by the registry was 7.8%. Of the 411 potentially eligible survivors identified, just over half (n = 232, 56% received their clinician's consent to be contacted. Of those 232 AYAs, 65% were unable to be contacted. Only 18 AYAs (7.8% refused permission for their contact details to be passed on to the research team. Of the 64 young people who agreed to be contacted, 50% (n = 32 completed the questionnaire. Conclusions Cancer registries which employ active clinician consent protocols may not be appropriate for recruiting large, representative samples of AYAs diagnosed with cancer. Given that AYA cancer survivors are highly mobile, alternative methods such as treatment centre and clinic based recruitment may need to be considered.

  13. Using block counts and distance sampling to estimate populations of chamois

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    Herrero, J.

    2011-12-01

    Full Text Available This study compared the efficacies of total block counts (BC and distance sampling (DS procedures to estimate the abundance of chamois populations in two mountain massifs, Posets and Maladeta, Spain. In 2002, 2006, 2007, and 2008, chamois populations were surveyed along itineraries above the timberline, twice per year: in July, after the parturition period, and in November, during the rut. The latter survey was used to estimate the sex ratio and to correct the proportion of males present in July. In 2008, poor weather prevented surveys in November. In the DS procedure, we used the data collected using BC and calculated the distances of the mapped groups to the itinerary, using a Geographical Information System. In Posets, estimates of population densities derived using BC ranged from 5.5 to 9.1 chamois km-2, while those derived using DS ranged from 7.5 to 9.7 chamois km-2. In Maladeta, the estimates ranged from 3.4 to 5.4 chamois km-2 (BC and from 4.6 to 8.5 chamois km-2 (DS. Coefficients of variation of DS ranged between 14% and 26%. In five of eight cases the counts of population size derived from BC were within the 95% confidence interval of the estimate derived from DS. In two of the other three cases, weather conditions created poor visibility during the rut, and few chamois were seen and, consequently, the rut sex ratio could not be estimated. BC provided objective, high-quality counts of chamois populations and it is easy to obtain, even if its efficacy can be constrained by the need of simultaneous itineraries and an underestimation of unknown magnitude. DS does not require sampling throughout the entire area above the timberline, and generates an estimate and a confidence interval; however, calculations require some skill and sample size must be high (n > 300 groups observed to produce a CV < 15%, which represents a sampling effort at least as large as the one derived from BC. BC represents a

  14. MICA Gene Deletion in 3411 DNA Samples from Five Distinct Populations in Mainland China and Lack of Association with Nasopharyngeal Carcinoma (NPC) in a Southern Chinese Han population.

    Science.gov (United States)

    Wang, WenYi; Tian, Wei; Zhu, FaMing; Li, LiXin; Cai, JinHong; Wang, Fan; Liu, KangLong; Jin, HeKun; Wang, JunLong

    2016-11-01

    Deletion of major histocompatibility complex class I chain-related genes A (MICA*Del) was investigated in 3,411 DNA samples from two southern Chinese Han populations (Hunan Han, HNH; Guangdong Han, GDH), two northern Chinese populations (Inner Mongolia Han, IMH; Inner Mongolia Mongol, IMM) and one southeastern Chinese Han population (Fujian Han, FJH) using an in-house polymerase chain reaction-sequence specific priming (PCR-SSP) assay, which enables direct discrimination between heterozygote and homozygote for MICA*Del. MICA*Del showed a frequency ranging from 0.8% in FJH to 5.7% in IMM (Pcorrected populations. In contrast to the association reported recently in a Taiwan Chinese population and a Malaysian Chinese cohort, MICA*Del distribution did not differ between 1,120 patients with nasopharyngeal carcinoma (NPC) and 1,483 normal controls in the HNH population (1.03% in NPC cases vs 1.18% in the controls, OR (95% CI) = 0.87 (0.51-1.47), p = 0.69). Further gender-stratified analysis also failed to disclose any male-specific association reported in a Taiwan Chinese population. Multi-locus typing of the 94 samples carrying MICA*Del revealed two new haplotypes, HLA-A*11:01-B*13:01-MICA*Del-MICB*009N-DRB1*04:06 and HLA-B*35:01-MICA*Del-MICB*009N-DRB1*15:01, in addition to HLA-B*48-MICA*Del. Unexpectedly, two samples with MICA*Del in the HNH population were each consistently found to have two distinct MICA alleles, indicating the existence of two MICA gene copies on certain HLA haplotypes. Based on the results from a sizeable case-control study, our data suggest that there is no association between MICA*Del and NPC in the southern Chinese Han population.

  15. Sampling

    CERN Document Server

    Thompson, Steven K

    2012-01-01

    Praise for the Second Edition "This book has never had a competitor. It is the only book that takes a broad approach to sampling . . . any good personal statistics library should include a copy of this book." —Technometrics "Well-written . . . an excellent book on an important subject. Highly recommended." —Choice "An ideal reference for scientific researchers and other professionals who use sampling." —Zentralblatt Math Features new developments in the field combined with all aspects of obtaining, interpreting, and using sample data Sampling provides an up-to-date treat

  16. Help-seeking in people with exceptional experiences: Results from a general population sample

    Directory of Open Access Journals (Sweden)

    Karin eLandolt

    2014-05-01

    Full Text Available Exceptional Experiences (EE are experiences that deviate from ordinary experiences, for example precognition, supernatural appearances or déjà vues. In spite of the high frequency of EE in the general population, little is known about their effect on mental health and about the way people cope with EE. This study aimed to assess the quality and quantity of EE in persons from the Swiss general population, to identify the predictors of their help seeking, and to determine how many of them approach the mental health system. An on-line survey was used to evaluate a quota sample of 1580 persons representing the Swiss general population with respect to gender, age, and level of education. Multinomial logistic regression was applied to integrate help-seeking, self-reported mental disorder, and other variables in a statistical model designed to identify predictors of help-seeking in persons with EE.Almost all participants (91% experienced at least one EE. Generally, help-seeking was more frequent when the EE were of negative valence. Help-seeking because of EE was less frequent in persons without a self-reported mental disorder (8.6% than in persons with a disorder (35.1% (OR = 5.7. Even when frequency and attributes of EE were controlled for, people without a disorder sought four times less often help because of EE than expected. Persons with a self-reported diagnosis of mental disorder preferred seeing a mental health professional. Multinomial regression revealed a preference for healers in women with less education, who described themselves as believing and also having had more impressive EE.Persons with EE who do not indicate a mental disorder less often sought help because of EE than persons who indicated a mental disorder. We attribute this imbalance to a high inhibition threshold to seek professional help. Moreover, especially less educated women did not approach the mental health care system as often as other persons with EE, but preferred

  17. Genetic restoration of a stocked brown trout Salmo trutta population using microsatellite DNA analysis of historical and contemporary samples

    DEFF Research Database (Denmark)

    Hansen, Michael Møller; Bekkevold, Dorte; Jensen, L.F.;

    2006-01-01

    -admixed individuals nevertheless remained in the wild-caught samples. In addition, two resident populations in isolated tributaries were found to be indigenous. The indigenous anadromous individuals from the Skjern River were unlikely to have been recruited from either the isolated tributary populations......1. Gene flow from domesticated to wild populations is a major threat to wild salmonid fish. However, few studies have addressed how populations could be restored after admixture has occurred. We analysed the prospects for restoring the previously intensively stocked brown trout population...... of the Skjern River, Denmark, by identifying remaining non-admixed individuals to be used for supportive breeding. 2. We analysed microsatellite DNA markers in historical (1940-50s) and contemporary (1992-2004) samples from the Skjern River system, from the strain of domesticated trout previously used...

  18. Diversity of nuclear short tandem repeat loci in representative sample of North-eastern Bosnian and Herzegovina population

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    Hadžiavdić Vesna

    2012-01-01

    Full Text Available Diversity of nuclear microsatellite markers were analyzed in a reference sample of the population of northeast Bosnia and Herzegovina. 437 samples taken from unrelated individuals were processed and three samples of paternity proof were shown. Detection effectiveness profile of the research, points to a valid choice of method of extraction, amplification and genotyping short tandem repeat (STR loci with PowerPlextm16 kit. Genetic analysis of allelic variants of the 15 STR loci PowerPlextm16 kit detected 17 samples determined as rare allelic variants or microvariants. Samples were divided into 15 different allelic variants at 7 different loci, and are: in locus D7S820, D16S539, D3S1358, D18S51, PENTA D, PENTA E and in locus vWA. Genetic analysis of mutations in cases of paternity determined three examples of single-step mutations in the loci FGA, Penta D and D3S1358. Genetic analysis of observed STR loci detected three allelic variant of genotype combination 7/10/11.3 in locus D7S820 Type II. Population genetic analysis of STR loci in a representative sample of the population of northeast Bosnia and Herzegovina included the application of the assessment tests of within-population genetic diversity and interpopulation diversity, as well as genetic differentiation between populations: North-eastern Bosnia and Herzegovina (BH and BH general reference, then the Croatian population, Macedonian, Serbian and Slovenian. Based on the result analysis of specific forensic parameters, it can be assumed that the most informative marker is PENTA E for population genetic analysis and forensic testing in the population of northeast Bosnia and Herzegovina. Research results fit regional STR database of this part of Europe.

  19. Genome-wide analysis in Brazilian Xavante Indians reveals low degree of admixture.

    Directory of Open Access Journals (Sweden)

    Patricia C Kuhn

    Full Text Available Characterization of population genetic variation and structure can be used as tools for research in human genetics and population isolates are of great interest. The aim of the present study was to characterize the genetic structure of Xavante Indians and compare it with other populations. The Xavante, an indigenous population living in Brazilian Central Plateau, is one of the largest native groups in Brazil. A subset of 53 unrelated subjects was selected from the initial sample of 300 Xavante Indians. Using 86,197 markers, Xavante were compared with all populations of HapMap Phase III and HGDP-CEPH projects and with a Southeast Brazilian population sample to establish its population structure. Principal Components Analysis showed that the Xavante Indians are concentrated in the Amerindian axis near other populations of known Amerindian ancestry such as Karitiana, Pima, Surui and Maya and a low degree of genetic admixture was observed. This is consistent with the historical records of bottlenecks experience and cultural isolation. By calculating pair-wise F(st statistics we characterized the genetic differentiation between Xavante Indians and representative populations of the HapMap and from HGDP-CEPH project. We found that the genetic differentiation between Xavante Indians and populations of Ameridian, Asian, European, and African ancestry increased progressively. Our results indicate that the Xavante is a population that remained genetically isolated over the past decades and can offer advantages for genome-wide mapping studies of inherited disorders.

  20. Genome-wide analysis in Brazilian Xavante Indians reveals low degree of admixture.

    Science.gov (United States)

    Kuhn, Patricia C; Horimoto, Andréa R V Russo; Sanches, José Maurício; Vieira Filho, João Paulo B; Franco, Luciana; Fabbro, Amaury Dal; Franco, Laercio Joel; Pereira, Alexandre C; Moises, Regina S

    2012-01-01

    Characterization of population genetic variation and structure can be used as tools for research in human genetics and population isolates are of great interest. The aim of the present study was to characterize the genetic structure of Xavante Indians and compare it with other populations. The Xavante, an indigenous population living in Brazilian Central Plateau, is one of the largest native groups in Brazil. A subset of 53 unrelated subjects was selected from the initial sample of 300 Xavante Indians. Using 86,197 markers, Xavante were compared with all populations of HapMap Phase III and HGDP-CEPH projects and with a Southeast Brazilian population sample to establish its population structure. Principal Components Analysis showed that the Xavante Indians are concentrated in the Amerindian axis near other populations of known Amerindian ancestry such as Karitiana, Pima, Surui and Maya and a low degree of genetic admixture was observed. This is consistent with the historical records of bottlenecks experience and cultural isolation. By calculating pair-wise F(st) statistics we characterized the genetic differentiation between Xavante Indians and representative populations of the HapMap and from HGDP-CEPH project. We found that the genetic differentiation between Xavante Indians and populations of Ameridian, Asian, European, and African ancestry increased progressively. Our results indicate that the Xavante is a population that remained genetically isolated over the past decades and can offer advantages for genome-wide mapping studies of inherited disorders.

  1. Excavating past population structures by surname-based sampling: the genetic legacy of the Vikings in northwest England.

    Science.gov (United States)

    Bowden, Georgina R; Balaresque, Patricia; King, Turi E; Hansen, Ziff; Lee, Andrew C; Pergl-Wilson, Giles; Hurley, Emma; Roberts, Stephen J; Waite, Patrick; Jesch, Judith; Jones, Abigail L; Thomas, Mark G; Harding, Stephen E; Jobling, Mark A

    2008-02-01

    The genetic structures of past human populations are obscured by recent migrations and expansions and have been observed only indirectly by inference from modern samples. However, the unique link between a heritable cultural marker, the patrilineal surname, and a genetic marker, the Y chromosome, provides a means to target sets of modern individuals that might resemble populations at the time of surname establishment. As a test case, we studied samples from the Wirral Peninsula and West Lancashire, in northwest England. Place-names and archaeology show clear evidence of a past Viking presence, but heavy immigration and population growth since the industrial revolution are likely to have weakened the genetic signal of a 1,000-year-old Scandinavian contribution. Samples ascertained on the basis of 2 generations of residence were compared with independent samples based on known ancestry in the region plus the possession of a surname known from historical records to have been present there in medieval times. The Y-chromosomal haplotypes of these 2 sets of samples are significantly different, and in admixture analyses, the surname-ascertained samples show markedly greater Scandinavian ancestry proportions, supporting the idea that northwest England was once heavily populated by Scandinavian settlers. The method of historical surname-based ascertainment promises to allow investigation of the influence of migration and drift over the last few centuries in changing the population structure of Britain and will have general utility in other regions where surnames are patrilineal and suitable historical records survive.

  2. Smoking, alcohol consumption, and drug use among adolescents with psychiatric disorders compared with a population based sample.

    Science.gov (United States)

    Mangerud, Wenche Langfjord; Bjerkeset, Ottar; Holmen, Turid Lingaas; Lydersen, Stian; Indredavik, Marit Sæbø

    2014-10-01

    This study investigated frequencies of smoking, alcohol use, and illicit drug use by diagnostic category in 566 adolescent psychiatric patients, comparing this sample with 8173 adolescents from the general population in Norway who completed the Young-HUNT 3 survey. Frequencies of current alcohol use were high in both samples but were lower among psychiatric patients. Compared with adolescents in the general population, adolescents in the clinical sample had a higher prevalence of current smoking and over four times higher odds of having tried illicit drugs. In the clinical sample, those with mood disorders reported the highest frequencies of smoking, alcohol use, and illicit drug use, whereas those with autism spectrum disorders reported the lowest frequencies. Our results show an increased prevalence of risky health behaviors among adolescents with psychiatric disorders compared with the general population. The awareness of disorder-specific patterns of smoking and substance use may guide preventive measures.

  3. Predicting Successful Aging in a Population-Based Sample of Georgia Centenarians

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    Jonathan Arnold

    2010-01-01

    Full Text Available Used a population-based sample (Georgia Centenarian Study, GCS, to determine proportions of centenarians reaching 100 years as (1 survivors (43% of chronic diseases first experienced between 0–80 years of age, (2 delayers (36% with chronic diseases first experienced between 80–98 years of age, or (3 escapers (17% with chronic diseases only at 98 years of age or older. Diseases fall into two morbidity profiles of 11 chronic diseases; one including cardiovascular disease, cancer, anemia, and osteoporosis, and another including dementia. Centenarians at risk for cancer in their lifetime tended to be escapers (73%, while those at risk for cardiovascular disease tended to be survivors (24%, delayers (39%, or escapers (32%. Approximately half (43% of the centenarians did not experience dementia. Psychiatric disorders were positively associated with dementia, but prevalence of depression, anxiety, and psychoses did not differ significantly between centenarians and an octogenarian control group. However, centenarians were higher on the Geriatric Depression Scale (GDS than octogenarians. Consistent with our model of developmental adaptation in aging, distal life events contribute to predicting survivorship outcome in which health status as survivor, delayer, or escaper appears as adaptation variables late in life.

  4. Is hypersexuality dimensional? Evidence for the DSM-5 from general population and clinical samples.

    Science.gov (United States)

    Walters, Glenn D; Knight, Raymond A; Långström, Niklas

    2011-12-01

    Hypersexual Disorder is currently being considered for inclusion in the DSM-5. To inform this process, we investigated the latent structure of the hypersexuality construct using Meehl's (1995) taxometric method. Data on sexual interests and behaviors were obtained from 2,101 general population males and females in Sweden and 716 male sex offenders from the United States. Taxometric analyses of self-report indicators of hypersexuality supported a dimensional interpretation of latent structure in both samples. These findings suggest that individual differences in hypersexuality are quantitative (matter of degree) rather than qualitative (difference in kind) in nature, at least when self-report data were used. This is another way of saying that hypersexuality is organized along a continuum of increasing sexual frequency and preoccupation, with clinical cases of hypersexuality falling at the upper end of the continuum or dimension. We conclude that the proposed inclusion of Hypersexual Disorder in the DSM-5 should acknowledge the lack of non-arbitrary breaks in the latent symptoms continuum which runs from very low to very high engagement in sexual behavior and preoccupation. The diagnostic threshold should therefore be decided from an analysis of external data on severity, comorbidity, and prognosis for individuals with sub-threshold and full diagnoses, respectively. Additionally, dimensional assessment of Hypersexual Disorder should be part of clinical diagnostic practice.

  5. Influenza virus drug resistance: a time-sampled population genetics perspective.

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    Matthieu Foll

    2014-02-01

    Full Text Available The challenge of distinguishing genetic drift from selection remains a central focus of population genetics. Time-sampled data may provide a powerful tool for distinguishing these processes, and we here propose approximate Bayesian, maximum likelihood, and analytical methods for the inference of demography and selection from time course data. Utilizing these novel statistical and computational tools, we evaluate whole-genome datasets of an influenza A H1N1 strain in the presence and absence of oseltamivir (an inhibitor of neuraminidase collected at thirteen time points. Results reveal a striking consistency amongst the three estimation procedures developed, showing strongly increased selection pressure in the presence of drug treatment. Importantly, these approaches re-identify the known oseltamivir resistance site, successfully validating the approaches used. Enticingly, a number of previously unknown variants have also been identified as being positively selected. Results are interpreted in the light of Fisher's Geometric Model, allowing for a quantification of the increased distance to optimum exerted by the presence of drug, and theoretical predictions regarding the distribution of beneficial fitness effects of contending mutations are empirically tested. Further, given the fit to expectations of the Geometric Model, results suggest the ability to predict certain aspects of viral evolution in response to changing host environments and novel selective pressures.

  6. Tangential-flow ultrafiltration: a versatile methodology for determination of complexation parameters in refractory organic matter from Brazilian water and soil samples.

    Science.gov (United States)

    Romão, L P C; Castro, G R; Rosa, A H; Rocha, J C; Padilha, P M; Silva, H C

    2003-04-01

    In this work the copper(II) complexation parameters of aquatic organic matter, aquatic and soil humic substances from Brazilian were determined using a new versatile approach based on a single-stage tangential-flow ultrafiltration (TF-UF) technique (cut-off 1 kDa) and sensitive atomic spectrometry methods. The results regarding the copper(II) complexation capacity and conditional stability constants obtained for humic materials were compared with those obtained using direct potentiometry with a copper-ion-selective electrode. The analytical procedure based on ultrafiltration is a good alternative to determine the complexation parameters in natural organic material from aquatic and soil systems. This approach presents additional advantages such as better sensibility, applicability for multi-element capability, and its possible to be used under natural conditions when compared with the traditional ion-selective electrode.

  7. Reducing bias in population and landscape genetic inferences: the effects of sampling related individuals and multiple life stages.

    Science.gov (United States)

    Peterman, William; Brocato, Emily R; Semlitsch, Raymond D; Eggert, Lori S

    2016-01-01

    In population or landscape genetics studies, an unbiased sampling scheme is essential for generating accurate results, but logistics may lead to deviations from the sample design. Such deviations may come in the form of sampling multiple life stages. Presently, it is largely unknown what effect sampling different life stages can have on population or landscape genetic inference, or how mixing life stages can affect the parameters being measured. Additionally, the removal of siblings from a data set is considered best-practice, but direct comparisons of inferences made with and without siblings are limited. In this study, we sampled embryos, larvae, and adult Ambystoma maculatum from five ponds in Missouri, and analyzed them at 15 microsatellite loci. We calculated allelic richness, heterozygosity and effective population sizes for each life stage at each pond and tested for genetic differentiation (F ST and D C ) and isolation-by-distance (IBD) among ponds. We tested for differences in each of these measures between life stages, and in a pooled population of all life stages. All calculations were done with and without sibling pairs to assess the effect of sibling removal. We also assessed the effect of reducing the number of microsatellites used to make inference. No statistically significant differences were found among ponds or life stages for any of the population genetic measures, but patterns of IBD differed among life stages. There was significant IBD when using adult samples, but tests using embryos, larvae, or a combination of the three life stages were not significant. We found that increasing the ratio of larval or embryo samples in the analysis of genetic distance weakened the IBD relationship, and when using D C , the IBD was no longer significant when larvae and embryos exceeded 60% of the population sample. Further, power to detect an IBD relationship was reduced when fewer microsatellites were used in the analysis.

  8. Hypnotic use in a population-based sample of over thirty-five thousand interviewed Canadians

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    Patten Scott B

    2006-11-01

    Full Text Available Abstract Background As with most medications, benzodiazepine and similar sedative hypnotics (BDZ/SSH can produce both beneficial and adverse effects. Pharmacoepidemiological studies have been limited in their capacity to evaluate the relationship between these medications and psychiatric diagnoses in non-clinical populations. The objective of this study was to provide a description of the pattern of use of BDZ/SSH medications in relation to both demographic and diagnostic data in a community population. Methods The source of data for this study was the Canadian Community Health Survey (CCHS 1.2, also known as the Canadian National Study of Mental Health and Well-being. This study was based on a nationally representative sample that included over 35 thousand subjects with a response rate of 77%. The survey interview included the latest version of the Composite International Diagnostic Interview (CIDI, which was developed for the World Health Organization's WHO Mental Health 2000 project. Current medication use was also recorded. Results As expected, BDZ/SSH use was more common in women than in men (4.2%, 95% CI 3.9 to 4.6 vs. 2.5%, 95% CI 2.2 to 2.8 and its frequency increased with age, 8.5% (95% CI 7.7 to 9.4 of those over the age of 65 compared to 2.4% (95% CI 2.2 to 2.7 of those aged 18 to 64 years. These medications were more frequently used in subjects with low levels of education (4.8%, 95% CI% 4.3 to 5.2 vs. high levels of education (2.4%, 95% CI 2.1 to 2.6 and low personal incomes (5.7%, 95% CI 5.2 to 6.3 vs. high personal incomes (2.3%, 95% CI 2.0 to 2.6. BDZ/SSH use was strongly associated with the presence of mood or anxiety disorders, but not with substance use disorders. Demographic differences persisted after statistical adjustment for diagnosis. Conclusion The observation that benzodiazepine use is more frequent in women, increases with age and is higher in low income and education groups supports previous findings. These results

  9. Piloting a Non-Invasive Genetic Sampling Method for Evaluating Population-Level Benefits of Wildlife Crossing Structures

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    Michael A. Sawaya

    2010-03-01

    Full Text Available Intuitively, wildlife crossing structures should enhance the viability of wildlife populations. Previous research has demonstrated that a broad range of species will use crossing structures, however, questions remain as to whether these measures actually provide benefits to populations. To assess this, studies will need to determine the number of individuals using crossings, their sex, and their genetic relationships. Obtaining empirical data demonstrating population-level benefits for some species can be problematic and challenging at best. Molecular techniques now make it possible to identify species, individuals, their sex, and their genetic relatedness from hair samples collected through non-invasive genetic sampling (NGS. We describe efforts to pilot a method to assess potential population-level benefits of wildlife crossing structures. We tested the feasibility of a prototype NGS system designed to sample hair from black bears (Ursus americanus and grizzly bears (U. arctos at two wildlife underpasses. The piloted hair-sampling method did not deter animal use of the trial underpasses and was effective at sampling hair from more than 90% of the bear crossing events at the underpasses. Hair samples were also obtained from non-target carnivore species, including three out of five (60% cougar (Puma concolor crossing events. Individual identification analysis revealed that three female and two male grizzly bears used one wildlife underpass, whereas two female and three male black bears were identified as using the other underpass. Of the 36 hair samples from bears analyzed, five failed, resulting in an 87% extraction success rate, and six more were only identified to species. Overall, 70% of the hair samples from bears collected in the field had sufficient DNA for extraction purposes. Preliminary data from our NGS suggest the technique can be a reliable method to assess the population-level benefits of Banff wildlife crossings. Furthermore, NGS

  10. Dental pain and associated factors in Brazilian preschoolers

    Science.gov (United States)

    Souza, João Gabriel Silva; Martins, Andrea Maria Eleutério de Barros Lima

    2016-01-01

    Abstract Objective: To describe the prevalence of dental pain in Brazilian preschoolers, as well as its associated factors, considering a representative sample of that population group in Brazil. Methods: Cross-sectional study that used the analytical data from national oral health survey (SB Brazil) carried out in 2010. A representative sample of Brazilian preschoolers aged 5 years was considered. Interviews were carried out (answered by parents/tutors), as well as clinical examinations in children. Descriptive, bivariate and mutiple analyzes were performed, described in odds ratios and 95% confidence interval (OR/95%CI). Results: 7280 preschoolers were included. Of these, 1520 (21.1%) had dental pain in the last 6 months. The chance of the occurrence of dental pain was higher among those who used dental services (1.51/1.02–2.23), with tooth decay (3.08/2.08–4.56), that self-perceveid the need for dental treatment (3.96/2.48–6.34), whose parents reported dissatisfaction by children with their teeth and mouth (1.47/1.04–2.10) and those who reported impact of oral problems on quality of life (5.76/3.90–8.49). Conclusions: The prevalence of dental pain among Brazilian preschool children was relatively high, being associated with the use of dental services and the normative and subjective oral health status. PMID:26965074

  11. Dental pain and associated factors in Brazilian preschoolers

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    João Gabriel Silva Souza

    Full Text Available Abstract Objective: To describe the prevalence of dental pain in Brazilian preschoolers, as well as its associated factors, considering a representative sample of that population group in Brazil. Methods: Cross-sectional study that used the analytical data from national oral health survey (SB Brazil carried out in 2010. A representative sample of Brazilian preschoolers aged 5 years was considered. Interviews were carried out (answered by parents/tutors, as well as clinical examinations in children. Descriptive, bivariate and mutiple analyzes were performed, described in odds ratios and 95% confidence interval (OR/95%CI. Results: 7280 preschoolers were included. Of these, 1520 (21.1% had dental pain in the last 6 months. The chance of the occurrence of dental pain was higher among those who used dental services (1.51/1.02–2.23, with tooth decay (3.08/2.08–4.56, that self-perceveid the need for dental treatment (3.96/2.48–6.34, whose parents reported dissatisfaction by children with their teeth and mouth (1.47/1.04–2.10 and those who reported impact of oral problems on quality of life (5.76/3.90–8.49. Conclusions: The prevalence of dental pain among Brazilian preschool children was relatively high, being associated with the use of dental services and the normative and subjective oral health status.

  12. Aetiology and severity of gingival recession in an adult population sample in Greece

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    Nikolaos Andreas Chrysanthakopoulos

    2011-01-01

    Full Text Available Background: Gingival recession is the most common and undesirable condition of the gingiva. The aim of study was to investigate the aetiology and severity of gingival recession in a Greek adult population sample. Methods : The study was performed on 165 males and 179 females, 18-68 years old who sought dental treatment in a private dental practice and showed gingival recession. All subjects were clinically examined and answered questions regarding their oral hygiene habits such as the type of toothbrush, frequency of brushing and method of brushing. The association between gingival recession and the following parameters was assessed: plaque score, gingival score and tooth position. Statistical analysis of the results was accomplished using chi-square test (α = 0.05. Results: The majority (79.4% of the patients showed grade I gingival recession and 15.3% showed grade II gingival recession. The maxillary 1 st and 2 nd molars (35.3% and the mandibular 1 st and 2 nd molars (28.7% were the teeth most frequently affected by root surface exposure. Patients with sub-gingival calculus, bacterial plaque and gingival inflammation (P < 0.05, malpositioned teeth (P < 0.001, horizontal brushing method, medium type of toothbrush (P < 0.001 and brushing once daily (P < 0.001 appeared to be the most common precipitating aetiological factor for gingival recession. Conclusion: According to the results of the present study, gingival recession was the result of more than one factor acting together. Horizontal brushing method, usage of medium type toothbrush and tooth brushing once daily were found to be more associated with gingival recession.

  13. Dietary patterns and food choices of a population sample of adults on Guam.

    Science.gov (United States)

    Pobocik, Rebecca S; Trager, Alison; Monson, Lora Morrell

    2008-01-01

    This study examined dietary patterns of adults on Guam. Four hundred subjects were selected via a multistage procedure to represent the island's population. A 24-hour dietary recall was administered via telephone in 1995 to 1996. Diets were computer analyzed. There were 4,913 food items reported, representing 1,042 individual foods. Most respondents, 51.3%, had a "more-frequent" eating pattern, 36.9% had "regular" and 11.8% had "less-frequent." Energy consumption increased (p<0.05) with eating frequency. Highest levels of all nutrients (p<0.05) were at the evening meal. The sexes did not differ in nutrient proportions by meal. Grains, meats, and beverages were eaten most frequently. Meat/fish, mixed dishes, and grains supplied 68% of the energy and 47% to 91% of the micronutrients. Twenty five percent of the carbohydrate was from sweetened drinks and desserts. Rice was the most frequently consumed food. More than half of the subjects had no fruit, a third had no vegetables, and only 38.5% had dairy foods. Calcium came from multiple sources: dairy (27.6%), mixed dishes (23.8%), fish (12.6%), desserts (8.5%) and vegetables (6.6%). Most people, 91.8%, had fiesta meals with median consumption at 6 meals per year (range: 0 to 200). Betel nut was used by 12.3% of the sample with median consumption 0 whole nuts per day (range: 0 to 25). Ethnic differences (p<0.01) were observed in both fiesta meal and betel nut consumption. The Guamanian diet includes limited use of traditional foods and dietary patterns associated with increased risk for chronic disease.

  14. Association of cardiometabolic risk factors and dental caries in a population-based sample of youths

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    Kelishadi Roya

    2010-04-01

    Full Text Available Abstract Background Cardiovascular disease (CVD risk factors begin from early life and track onto adulthood. Oral and dental diseases share some risk factors with CVD, therefore by finding a clear relation between dental diseases and cardiometabolic risk factors; we can then predict the potential risk of one based on the presence of the other. This study aimed to compare the prevalence of dental caries between two groups of age-matched adolescents with and without CVD risk factors. Methods In this case-control study, the decayed, missing and filled surfaces (DMFS, based on the criteria of the World Health Organization, were compared in two groups of equal number (n = 61 in each group of population-based sample of adolescents with and without CVD risk factors who were matched for sex and age group. Results The study participants had a median age 13 y 5 mo, age range 11 y 7 mo to 16 y 1 mo, with male-to-female proportion of 49/51. We found significant difference between the mean values of DMFS, body mass index, waist and hip circumferences, as well as serum lipid profile in the case and control groups. Significant correlations were documented for DMFS with TC (r = 0.54, p = 0.02, LDL-C (r = 0.55, p = 0.01 and TG (r = 0.52, p = 0.04 in the case group; with LDL-C (r = 0.47, p = 0.03 in the whole study participants and with TC in control s(r = 0.45, p = 0.04. Conclusions Given the significant associations between dental caries and CVD risk factors among adolescents, more attention should be paid to oral health, as one of the topics to be taken into account in primordial/primary prevention of cardiometabolic disorders.

  15. Treatment and survival in a population-based sample of patients diagnosed with gastroesophageal adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Deirdre P Cronin-Fenton; Margaret M Mooney; Limin X Clegg; Linda C Harlan

    2008-01-01

    AIM:To examine the extent of use of specific therapies in clinical practice,and their relationship to therapies validated in clinical trials.METHODS:The US National Cancer Institutes' Patterns of Care study was used to examine therapies and survival of patients diagnosed in 2001 with histologically-confirmed gastroesophageal adenocarcinoma (n = 1356).The study re-abstracted data and verified therapy with treating physicians for a population-based stratified random sample.RESULTS:Approximately 62% of patients had stomach adenocarcinoma (SAC),while 22% had gastric-cardia adenocarcinoma (GCA),and 16% lower esophageal adenocarcinoma (EAC).Stage IV/ unstaged esophageal cancer patients were most likely and stage I -111 stomach cancer patients least likely to receive chemotherapy as all or part of their therapy;gastric-cardia patients received chemotherapy at a rate between these two.In multivariable analysis by anatomic site,patients 70 years and older were significantly less likely than younger patients to receive chemotherapy alone or chemoradiation for all three anatomic sites.Among esophageal and stomach cancer patients,receipt of chemotherapy was associated with lower mortality;but no association was found among gastric-cardia patients.CONCLUSION:This study highlights the relatively low use of clinical trials-validated anti-cancer therapies in community practice.Use of chemotherapy-based treatment was associated with lower mortality,dependent on anatomic site.Findings suggest that physicians treat lower esophageal and SAC as two distinct entities,while gastric-cardia patients receive a mix of the treatment strategies employed for the two other sites.

  16. DIETARY SODIUM INTAKE IN A SAMPLE OF ADULT MALE POPULATION IN SOUTHERN ITALY.Results of the Olivetti Heart Study

    OpenAIRE

    Strazzullo, P; Venezia, Antonella; Barba, Gianvincenzo; Russo, Ornella; Capasso, Clemente; De Luca, Viviana; Farinaro, Eduardo; Cappuccio, Francesco P; Galletti, Ferruccio; Rossi, Giovanni

    2010-01-01

    Abstract Objective: To assess dietary habitual sodium intake, the association between daily sodium intake and anthropometric indices, food habits and hypertension in the sample of adult male population participating in the Olivetti Heart Study. Design, Setting and Participants: The study population was composed of 940 men participating in the 2002-04 follow-up examination of the Olivetti Heart Study. Blood pressure, anthropometric indices, biochemical parameters and sodium excre...

  17. Inferring Population Size History from Large Samples of Genome-Wide Molecular Data - An Approximate Bayesian Computation Approach.

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    Simon Boitard

    2016-03-01

    Full Text Available Inferring the ancestral dynamics of effective population size is a long-standing question in population genetics, which can now be tackled much more accurately thanks to the massive genomic data available in many species. Several promising methods that take advantage of whole-genome sequences have been recently developed in this context. However, they can only be applied to rather small samples, which limits their ability to estimate recent population size history. Besides, they can be very sensitive to sequencing or phasing errors. Here we introduce a new approximate Bayesian computation approach named PopSizeABC that allows estimating the evolution of the effective population size through time, using a large sample of complete genomes. This sample is summarized using the folded allele frequency spectrum and the average zygotic linkage disequilibrium at different bins of physical distance, two classes of statistics that are widely used in population genetics and can be easily computed from unphased and unpolarized SNP data. Our approach provides accurate estimations of past population sizes, from the very first generations before present back to the expected time to the most recent common ancestor of the sample, as shown by simulations under a wide range of demographic scenarios. When applied to samples of 15 or 25 complete genomes in four cattle breeds (Angus, Fleckvieh, Holstein and Jersey, PopSizeABC revealed a series of population declines, related to historical events such as domestication or modern breed creation. We further highlight that our approach