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Sample records for boheman coleoptera chrysomelidae

  1. Toxicity and repellency of essential oils to Zabrotes subfasciatus (Boheman) (Coleoptera, Chrysomelidae, Bruchinae) in Phaseolus vulgaris L Toxicidade e repelência de óleos essenciais a Zabrotes subfasciatus (Boheman) (Coleoptera, Chrysomelidae, Bruchinae) em grãos de Phaseolus vulgaris L

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    Solange Maria de França; José Vargas de Oliveira; Alberto Belo Esteves Filho; Cynara Moura de Oliveira

    2012-01-01

    The effects of tangerine (Phaseolus vulgaris Blanco), lemon (Citrus medica limonum Lush), pear orange (Citrus sinensis L. Osbeck), red copaiba (Copaifera langsdorffii Desf.), rosemary (Baccharis dracunculifolia De Candole), Eucalyptus (Eucalyptus globulus Labillardière and E. citriodora Hook), lemongrass (Cymbopogon citratus Stapf.) and citronella (Cimbopogon nardus Linnaeus) oils at several concentrations on Zabrotes subfasciatus (Boheman) were studied. In toxicity tests, grains of Phaseolus...

  2. Bionomics data and descriptions of the immatures of Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), pest of the oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) (Arecaceae) in Tabasco, Mexico; Datos bionomicos y descripcion de los inmaduros de Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), plaga de la Palma Aceitera (Elaeis guineensis J.) y de la Palma Camedor (Chamaedorea elegans Mart.) (Arecaceae) en Tabasco, Mexico

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    Cordova-Ballona, Leonides [Universidad Popular de la Chontalpa, Tabasco (Mexico); Sanchez-Soto, Saul [Colegio de Postgraduados, Tabasco (Mexico). Campus Tabasco

    2008-11-15

    Characters of the external morphology of egg, larval instars and pupae of Calyptocephala gerstaeckeri Boheman, insect pest of oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) in the State of Tabasco, Mexico, are described and illustrated. Some bionomics data are also presented. The specie was reared in the laboratory on young oil palm plants. (author)

  3. Toxicity and repellency of essential oils to Zabrotes subfasciatus (Boheman (Coleoptera, Chrysomelidae, Bruchinae in Phaseolus vulgaris L Toxicidade e repelência de óleos essenciais a Zabrotes subfasciatus (Boheman (Coleoptera, Chrysomelidae, Bruchinae em grãos de Phaseolus vulgaris L

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    Solange Maria de França

    2012-09-01

    Full Text Available The effects of tangerine (Phaseolus vulgaris Blanco, lemon (Citrus medica limonum Lush, pear orange (Citrus sinensis L. Osbeck, red copaiba (Copaifera langsdorffii Desf., rosemary (Baccharis dracunculifolia De Candole, Eucalyptus (Eucalyptus globulus Labillardière and E. citriodora Hook, lemongrass (Cymbopogon citratus Stapf. and citronella (Cimbopogon nardus Linnaeus oils at several concentrations on Zabrotes subfasciatus (Boheman were studied. In toxicity tests, grains of Phaseolus vulgaris L. cv. Rajadinho were impregnated with oils and infested with adults of Z. subfasciatus up to 24 hours old. All tested oils were effective in reducing the viable egg-laying and adult emergence of this pest, in function of the concentrations used, highlighting E. citriodora and E. globulus oils which caused 100% effectiveness from 0.5 mL Kg-1 concentration. In repellency tests, two arenas consisting of plastic containers, connected symmetrically to a central box by two plastic tubes were used. In one of the boxes, untreated beans were placed and on the other ones beans treated with each oil concentration were used. In the central box, five couples of Z. subfasciatus were released. Grains of P. vulgaris treated with oils of E. citriodora, C. citratus and C. oleifera reduced the attraction percentage of Z. subfasciatus adults, while the E. globulus increased this percentage. The percentages of reduced viable eggs ranged from 17.9% (C. medica limonum to 93.3% (C. nardus, while the reduction on the number of emerged insects was 23.9% and 95.9%, respectively for these same oils.Estudaram-se os efeitos dos óleos de tangerina 'Cravo' (Phaseolus vulgaris Blanco, limão-siciliano (Citrus medica limonum Lush, laranja 'Pêra' (Citrus sinensis L. Osbeek, copaíba-vermelha (Copaifera langsdorffii Desf., alecrim-do-campo (Baccharis dracunculifolia De Candole, eucalipto (Eucalyptus globulus Labillardière e Eucalyptus citriodora Hook, capim-santo (Cymbopogon citratus

  4. Bionomics data and descriptions of the immatures of Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), pest of the oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) (Arecaceae) in Tabasco, Mexico

    International Nuclear Information System (INIS)

    Characters of the external morphology of egg, larval instars and pupae of Calyptocephala gerstaeckeri Boheman, insect pest of oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) in the State of Tabasco, Mexico, are described and illustrated. Some bionomics data are also presented. The specie was reared in the laboratory on young oil palm plants. (author)

  5. Host plant preference in Colorado potato beetle (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Field and laboratory-choice tests were conducted to better understand host plant preference by the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), in Virginia. In laboratory olfactometer studies, L. decemlineata preferred potato over both tomato and eggplant foli...

  6. Dimorfismo Sexual de Onchoscelis germari (Boheman (Coleoptera: Curculionidae

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    Marliton Barreto

    2012-12-01

    Abstract. Onchoscelis germari (Boheman is a weevil species associated with Simarouba amara Aubl (Simaroubaceae, commonly named “caixeta”. Sixty three specimens were collected and observed in laboratory for sexual dimorphism differences. The following morphological aspects were observed and illustrated: 1 rostrum of males more rough, coarse and with more dense punctures, tricarinate at the basal 2/3, and covered with erect scales from its base to near middle; rostrum of females smoother, with fine and sparse punctures, only the median longitudinal distinct, and only the base covered by erect scales; 2 males with antenna inserted near the apex of rostrum; and near the middle in females; 3 sternal channel of males flatter, with its lateral margins only indicated or absent in the prosternum; the sternal channel of the females deeper, with its lateral margins more prominent; 4 the eighth abdominal tergite of males visible and distinct; while in the females covered by the seventh abdominal tergite.

  7. Noteworthy records of Hispines from Belize (Coleoptera: Chrysomelidae)

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    Naczi, Robert F. C.; Charles L. Staines

    2011-01-01

    Cephaloleia consanguinea Baly, Cephaloleia fulvolimbata Baly, Cephaloleia ruficollis Baly, Chalepus amabilis Baly, Chalepus brevicornis (Baly), Chalepus pici Descarpentries and Villiers, Microrhopala erebus (Newman), Octhispa bimaculata Uhmann, Octotoma championi Baly, Pseudispa tuberculata Staines, Sceloenopla erudita (Baly), Stenispa guatemalensis Uhmann, Sumitrosis gestroi (Weise), and Sumitrosis terminatus (Baly) (Coleoptera: Chrysomelidae: Cassidinae) are new country records of hispine c...

  8. Shared Ancestry of Symbionts? Sagrinae and Donaciinae (Coleoptera, Chrysomelidae) Harbor Similar Bacteria

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    Dimitra Synefiaridou; Gregor Kölsch

    2012-01-01

    When symbioses between insects and bacteria are discussed, the origin of a given association is regularly of interest. We examined the evolution of the symbiosis between reed beetles (Coleoptera, Chrysomelidae, Donaciinae) and intracellular symbionts belonging to the Enterobacteriaceae. We analyzed the partial sequence of the 16S rRNA to assess the phylogenetic relationships with bacteria we found in other beetle groups (Cerambycidae, Anobiidae, other Chrysomelidae). We discuss the ecology of...

  9. Utility of morphological and molecular techniques for determination of paternity in two subspecies of Diabrotica undecimpunctata (Coleoptera: Chrysomelidae)

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    An experiment was conducted to determine the paternity of F1 progeny using morphological and molecular methods in Diabrotica (Coleoptera: Chrysomelidae) subspecies: Diabrotica undecimpunctata howardi Barber, also known as spotted cucumber beetle and D. undecimpunctata undecimpunctata Mannerheim, als...

  10. Effects of irradiated diet on longevity and prolificity of successive generations of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae)

    International Nuclear Information System (INIS)

    The study was done in the laboratory of Entomology of the Centro de Energia Nuclear na Agricultura (CENA), University of Sao Paulo, in Piracicaba, State of Sao Paulo, Brazil. This research was carried out to observe the effect of irradiated beans (Phaseolus vulgaris, (L.), cultivar Rosinha) on the longevity and prolificity of Zabrotes subfasciatus (Boheman, 1833) (Coleoptera, Bruchidae). The first part of the study included the rearing of five generations of Zabrotes subfasciatus which were fed with irradiated beans (0, 10, 20, 100 e 200 krad, with a dose rate of approximately 70 krad/h). The second one consisted of growth of seven generations on irradiated beans (0, 100, 500, 1500 and 3000 krad, with a dose rate of approximately 70 krad/h). The data were obtained through daily countings of insect birth and death rates. It was concluded that irradiation modified the nutritional quality of beans, affecting the development of Zabrotes subfasciatus. This effect was studied through the longevity and prolificity, which were altered as compared with the control, once they were higher for the dosis of 1500 and 3000 krad. (author)

  11. The Leaf-Beetles (Coleoptera, Chrysomelidae) Feed On Some Weeds In Tokat Province

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    Çam, Halit; ATAY, Turgut

    2004-01-01

    This study was carried out to determine the leaf-beetles (Coleoptera, Chrysomelidae) which causes extensive damage on weeds in the vicinity of Tokat, Turkey. Total 9 taxa including 4 species of Chrysomelinae, 1 species of Clytrinae, 1 species of Criocerinae, 2 species of Alticinae and 1 species of Cassidinae were found to be destructive on different weed species. These species were; Entomoscelis adonidis (Pall.) on Sinapis arvensis L., Gastrophysa polygoni (L.) on Polygonum convolvulus L. an...

  12. Evolution of exocrine chemical defense in leaf beetles (Coleoptera: Chrysomelidae)

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    Pasteels, Jacques M.; Rowell-Rahier, Martine; Braekman, J.C.; Daloze, D.; Duffey, S.

    2009-01-01

    In this review we speculate on possible scenarios for the evolution of the very high diversity in chemical compounds liberated by exocrine glands of adults Chrysomelidae. Shift in host plant affinities and subsequent adaptation of the beetles to the plant toxins strongly influence the nature of the beetles' chemical defense.

  13. Diversity and altitudinal distribution of Chrysomelidae (Coleoptera in Peregrina Canyon, Tamaulipas, Mexico

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    Uriel Jeshua Sánchez-Reyes

    2014-06-01

    Full Text Available The Chrysomelidae (Coleoptera is a highly speciose family that has been poorly studied at the regional level in Mexico. In the present study, we estimated species richness and diversity in oak-pine forest, Tamaulipan thorny scrub and in tropical deciduous forests in Peregrina Canyon within the Altas Cumbres Protected Area of the northeastern state of Tamaulipas, Mexico. Sampling of Chrysomelidae consisted of five sweep net samples (200 net sweeps within each of three sites during four sample periods: early dry season, late dry season, early wet season, and late wet season. Species were identified and total numbers per species were recorded for each sample. A total of 2,226 specimens were collected belonging to six subfamilies, 81 genera and 157 species of Chrysomelidae from the study area. Galerucinae was the most abundant subfamily with 1,828 specimens, representing 82.1% of total abundance in the study area. Lower abundance was recorded in Cassidinae (8.5%, Eumolpinae (3.6%, Cryptocephalinae (2.2%, Chrysomelinae (2.2%, and finally Criocerinae (1.3%. The highest species richness was also presented in the subfamily Galerucinae with 49% of the total obtained species followed by Cassidinae (20%, Cryptocephalinae (9.7%, Eumolpinae (9.7%, Chrysomelinae (6.5% and Criocerinae (5.2%. The most common species were Centralaphthona fulvipennis Jacoby (412 individuals, Centralaphthona diversa (Baly (248, Margaridisa sp.1 (219, Acallepitrix sp.1 (134, Longitarsus sp.1 (104, Heterispa vinula (Erichson (91, Epitrix sp.1 (84 and Chaetocnema sp.1 (72. Twenty-two species were doubletons (1.97% of total abundance and 52 were singletons (2.33%. The estimated overall density value obtained was 0.0037 individuals/m2. The greatest abundance and density of individuals were recorded at the lowest elevation site. However, alpha diversity increased with increasing altitude. Similarity values were less than 50% among the three sites indicating that each site had distinct

  14. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

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    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  15. Plagiometriona emarcida (Boheman, 1855) and Plagiometriona forcipata (Boheman, 1855) (Coleoptera: Chrysomelidae: Cassidinae), a single species differing in larval performace and adult phenotype

    Czech Academy of Sciences Publication Activity Database

    Flinte, V.; Windsor, D.; Sekerka, Lukáš; de Macedo, M. V.; Monteiro, R. F.

    2010-01-01

    Roč. 44, 15-16 (2010), s. 891-904. ISSN 0022-2933 Institutional research plan: CEZ:AV0Z50070508 Keywords : Cassidinae * Plagiometriona * synonymy Subject RIV: EG - Zoology Impact factor: 0.782, year: 2010

  16. Estudos bioecológicos de Syphraea uberabensis (Coleoptera: Chrysomelidae Bechyné 1956

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    Charles Wikler

    2005-06-01

    Full Text Available Tibouchina herbacea (DC. Cog. é uma planta ornamental introduzida propositadamente no arquipélago do Havaí e, devido à ausência de inimigos naturais e condições edafo-climáticas adequadas, está se dispersando rapidamente pelas florestas nativas e regiões úmidas das principais ilhas havaianas. Por ser originária do Brasil, foram realizadas diversas viagens exploratórias em busca de por inimigos naturais específicos que pudessem controlar esta planta. Dentre os agentes selecionados com impacto e potencial de especificidade à T. herbacea encontra-se Syphraea uberabensis (Bechyne, 1955 (Coleoptera: Chrysomelidae. Este trabalho visa estudos sobre a biologia, ecologia e especificidade deste inseto e também uma avaliação criteriosa dos possíveis impactos causados por este inimigo natural na população da planta.

  17. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae) *

    Science.gov (United States)

    Petitpierre, Eduard

    2011-01-01

    Abstract Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  18. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae).

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    Petitpierre, Eduard

    2011-01-01

    Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xy(p) and XY(p) clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry. PMID:22303104

  19. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae

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    Eduard Petitpierre

    2011-12-01

    Full Text Available Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%, but with the XO well represented too (19.75%. The modal haploid number for chrysomelines is n = 12 (34.2% although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6% and in subtribe Chrysomelina n = 17 (65.7%. Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74 has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry.

  20. Evaluation of the Boll Weevil Anthonomus grandis Boheman (Coleoptera: Curculionidae) suppression program in the state of Goiás, Brazil.

    Science.gov (United States)

    de Lima, I S; Degrande, P E; Miranda, J E; dos Santos, W J

    2013-02-01

    The boll weevil Anthonomus grandis Boheman (Coleoptera: Curculionidae) is the most important cotton pest in Brazil. A large-scale field-testing of a Boll Weevil Suppression Program (BWSP) was implemented to assess its technical and operational feasibility for boll weevil suppression in the state of Goiás, Brazil. The pilot plan focused on 3,608 ha of cotton during the 2006/2007 and 6,011 ha in the 2007/2008 growing seasons; the areas were divided into four inner zones with an outer buffer zone. We analyzed data on boll weevil captures using pheromone traps installed in the BWSP fields, on the detection of the first insect and the first damaged floral bud, greatest damage, and number of insecticide applications. The nonparametric Mann-Whitney U test was used to evaluate the differences between presuppression and suppression years. Fourteen pheromone-baited trapping evaluations were used to compare the weevil populations from 2006/2007 and 2007/2008 growing seasons. The BWSP regime reduced in-season boll weevil captures from 15- to 500-fold compared to presuppression levels in the preceding year. The low capture rates were related to delays in infestation and damage by weevils. The smaller population size measured by trapping and field monitoring reduced the number of required insecticide treatments. The BWSP strategy was efficient in suppressing populations of this pest and is a viable program for cotton production in subtropical and tropical regions, with long-term economic and environmental benefits. PMID:23949716

  1. Immunofluorescence localization and ultrastructure of Stewart’s wilt disease bacterium Pantoea stewartii in maize leaves and in its flea beetle vector Chaetocnema pulicaria (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Pantoea stewartii is the causal agent of Stewart's wilt of sweet corn, the most serious bacterial disease of sweet corn and maize in the North-Central and Eastern USA. P. stewartii is transmitted mainly by the corn flea beetle Chaetocnema pulicaria (Coleoptera: Chrysomelidae) and this bacterium is a...

  2. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and D. barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data

    Science.gov (United States)

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica v. virgifera (16,747 bp) and D. barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and...

  3. Releases, distribution and abundance of Gratiana boliviana (Coleoptera:Chrysomelidae), a biological control agent of tropical soda apple (Solanum viarum, Solanaceae), in Florida

    Science.gov (United States)

    A biological control program against tropical soda apple (TSA) (Solanum viarum Dunal (Solanaceae)) released 176,643 Gratiana boliviana Spaeth (Coleoptera: Chrysomelidae) in Florida from 2003 to 2008. The spatial distribution of releases was clustered with more beetles released in south/central Flor...

  4. Molecular identification of Epitrix potato flea beetles (Coleoptera: Chrysomelidae) in Europe and North America.

    Science.gov (United States)

    Germain, Jean-François; Chatot, Catherine; Meusnier, Isabelle; Artige, Emmanuelle; Rasplus, Jean-Yves; Cruaud, Astrid

    2013-06-01

    Epitrix species (Coleoptera: Chrysomelidae) feed mostly on plants from the family Solanaceae and some of them are major pests of potato crops. All Epitrix species are morphologically highly similar, which makes them difficult to identify and limits their study and management. Identification of species is mostly based on the observation of the genitalia and requires a high level of expertise. Here, we propose a tool to reliably identify all developmental stages of the most economically important Epitrix species feeding on potato in Europe and North America (Epitrix cucumeris, Epitrix similaris, Epitrix tuberis, Epitrix subcrinita and Epitrix hirtipennis). We first sequenced two DNA markers (mitochondrial cytochrome c oxidase I (COI) and nuclear internal transcribed spacer 2 (ITS2)) to test their effectiveness in differentiating among six Epitrix species (126 specimens). Morphospecies of Epitrix were well-differentiated by both DNA barcodes and no mitochondrial introgression was detected. Then, we developed an RFLP-based diagnostic method and showed that unambiguous species discrimination can be achieved by using the sole restriction enzyme TaqI on COI polymerase chain reaction products. The tool proposed here should improve our knowledge about Epitrix species biology, distribution and host range, three capacities that are particularly important in the detection and management of these pest species. Specifically, this tool should help prevent the introduction of E. tuberis and E. subcrinita in Europe and limit the spread of the recently introduced E. cucumeris and E. similaris, with minimal disruption to Solanaceae trade. PMID:23448201

  5. Sustainable management tactics for control of Phyllotreta cruciferae (Coleoptera: Chrysomelidae) on canola in Montana.

    Science.gov (United States)

    Reddy, Gadi V P; Tangtrakulwanich, Khanobporn; Miller, John H; Ophus, Victoria L; Prewett, Julie

    2014-04-01

    The crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), has recently emerged as a serious pest of canola (Brassica napus L.) in Montana. The adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. In 2013, damage to canola seedlings was high (approximately 80%) in many parts of Montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. In the current study, the effectiveness of several biopesticides was evaluated and compared with two insecticides (deltamethrin and bifenthrin) commonly used as foliar sprays as well as seed treatment with an imidacloprid insecticide for the control of P. cruciferae under field conditions in 2013. The biopesticides used included an entomopathogenic nematode (Steinernema carpocapsae), two entomopathogenic fungi (Beauveria bassiana and Metarhizium brunneum), neem, and petroleum spray oils. The control agents were delivered in combination or alone in a single or repeated applications at different times. The plant-derived compound neem (azadirachtin), petroleum spray oil, and fatty acids (M-Pede) only showed moderate effect, although they significantly reduced leaf injuries caused by P. cruciferae and resulted in higher canola yield than the untreated control. Combined use of B. bassiana and M. brunneum in two repeated applications and bifenthrin in five applications were most effective in reducing feeding injuries and improving yield levels at both trial locations. This indicates that entomopathogenic fungi are effective against P. cruciferae, and may serve as alternatives to conventional insecticides or seed treatments in managing this pest. PMID:24772547

  6. Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains.

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    Demirci, Meryem; Sevim, Elif; Demir, İsmail; Sevim, Ali

    2013-05-01

    Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae) is an important forest pest which damages many trees such as willow, poplar, and hazelnut. In order to find new microbes that can be utilized as a possible microbial control agent against this pest, we investigated the culturable bacterial flora of it and tested the isolated bacteria against P. versicolora larvae and adults. We were able to isolate nine bacteria from larvae and adults. The isolates were characterized using a combination of morphological, biochemical, and physiological methods. Additionally, we sequenced the partial sequence of the 16S rRNA gene to verify conventional identification results. Based on characterization studies, the isolates were identified as Staphylococcus sp. Pv1, Rahnella sp. Pv2, Rahnella sp. Pv3, Rahnella sp. Pv4, Rahnella sp. Pv5, Pantoea agglomerans Pv6, Staphylococcus sp. Pv7, Micrococcus luteus Pv8, and Rahnella sp. Pv9. The highest insecticidal activity against larvae and adults was obtained from M. luteus Pv8 with 50 and 40 % mortalities within 10 days after treatment, respectively. Extracellular enzyme activity of the bacterial isolates such as amylase, proteinase, lipase, cellulose, and chitinase was also determined. Consequently, our results show that M. luteus Pv8 might be a good candidate as a possible microbial control agent against P. versicolora and were discussed with respect to biocontrol potential of the bacterial isolates. PMID:23054688

  7. Diel Patterns of Colaspis brunnea and Colaspis crinicornis (Coleoptera: Chrysomelidae) in Southeastern Nebraska.

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    Miwa, Kentaro; Meinke, Lance J

    2015-12-01

    A field study was conducted to increase our understanding of diel activity patterns of Colaspis brunnea (F.) and Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) in key crop habitats. Within 24-h periods, C. brunnea was sampled in clover fields (primarily red clover, Trifolium pretense (L.), with some sweet clover, Melilotus officinalis (L.) Pallas, and downy brome, Bromus tectorum (L.)) and soybean, Glycine max (L.) Merrill, fields, using a sweep-net, while whole-plant-count sampling was used to monitor C. crinicornis densities in field corn, Zea mays (L.). Sweep-net captures of C. brunnea were significantly greater at night than during the day, suggesting possible vertical movement within the canopy during a 24-h period. Colaspis crinicornis densities on corn plants were fairly constant throughout a 24-h period, but beetle activity (e.g., walking, mating) was significantly greater at night than during the day. Results suggest that both Colaspis species may be exhibiting similar increases in activity at night that facilitates movement from more protected to more exposed areas within a habitat. It is unclear what mechanisms drive this diel pattern, but vegetation architecture and associated interactions with environmental conditions may play a role. Sweep-netting in clover or soybean fields and use of whole-plant-counts in cornfields were effective sampling methods for Colaspis adults. However, because activity and behaviors of Colaspis beetles were influenced by time of day in this study, use of a consistent sampling time within a diel period would be recommended for future population studies or integrated pest management decision-making. PMID:26314034

  8. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae of an Ecuadorian Mountain Forest Using DNA Barcoding.

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    Birthe Thormann

    Full Text Available Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs (n = 284-289. Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2 and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons, the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a

  9. Endophyte isolate and host grass effects on Chaetocnema pulicaria (Coleoptera: Chrysomelidae) feeding.

    Science.gov (United States)

    Ball, Olivier J P; Gwinn, Kimberly D; Pless, Charles D; Popay, Alison J

    2011-04-01

    Endophytic fungi belonging to the genus Neotyphodium, confer resistance to infected host grasses against insect pests. The effect of host species, and endophtye species and strain, on feeding and survival of the corn flea beetle, Chaetocnema pulicaria Melsheimer (Coleoptera: Chrysomelidae) was investigated. The grass-endophyte associations included natural and artificially derived associations producing varying arrays of common endophyte-related alkaloids or alkaloid groups, peramine, lolitrem B, ergovaline, and the lolines. Preference and nonpreference tests showed that C. pulicaria feeding and survival were reduced by infection of tall fescue with the wild-type strain of N. coenophialum, the likely mechanism being antixenosis rather than antibiosis. In the preference tests, endophyte and host species effects were observed. Of the 10 different Neotyphodium strains tested in artificially derived tall fescue associations, eight strongly deterred feeding by C. pulicaria, whereas the remaining two strains had little or no effect on feeding. Infection of tall fescue with another fungal symbiont, p-endophyte, had no effect. Perennial ryegrass, Lolium perenne L., infected with six strains of endophyte, was moderately resistant to C. pulicaria compared with endophyte-free grass, but four additional strains were relatively inactive. Six Neotyphodium-meadow fescue, Festuca pratensis Huds., associations, including the wild-type N. uncinatum-meadow fescue combination, were resistant, whereas three associations were not effective. Loline alkaloids seemed to play a role in antixenosis to C. pulicaria. Effects not attributable to the lolines or any other of the alkaloids examined also were observed. This phenomenon also has been reported in tests with other insects, and indicates the presence of additional insect-active factors. PMID:21510220

  10. A Temperature-Dependent Development Model for Willow Beetle Species (Coleoptera: Chrysomelidae) in Ireland: Simulation of Phenology/Voltinism in Response to Climate Change

    OpenAIRE

    Pollard, Ciaran P.

    2015-01-01

    Rising fossil fuel prices, energy security and adherence to existing European Union (EU) climate/energy policies means that Ireland must look towards alternative energy sources to meet future demand. Woody biomass in the form of short rotation coppice willow (SRCW) is considered a viable option. SRCW is vulnerable to damage by a range of diseases and pests however. The blue (Phratora vulgatissima) and brown (Galerucella lineola) willow beetles (Coleoptera: Chrysomelidae) are identified econom...

  11. Mortality and impaired development of Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) induced by extract of sangra d’água Croton urucurana Baill (Euphorbiaceae).

    OpenAIRE

    Gabriel dos Santos Carvalho; Leonardo Santana da Silva; Luciana Barboza Silva; Mayra Layra dos Santos Almeida; Bruno Ettore Pavam; Marize Terezinha Lopes Pereira Peres

    2014-01-01

    Phaseolus vulgaris L., the green bean, is an important source of protein and minerals in the diet of the population. But its productivity is affected by the attack of numerous pests, from emergence to storage, Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae) being the major pest of stored beans. In the search for alternatives to chemical control of this insect and considering the potential biological activity of plant secondary metabolites, this study was conducted in order to evaluate...

  12. Eficiência do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae na cultura da batata Performance of the insectide chlorpyrifos to control Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato crop

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    Luiz Antonio Salles

    1999-06-01

    Full Text Available Foi avaliado o desempenho do inseticida clorpirifós no controle de larvas de Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, na cultura da batata. O experimento foi desenvolvido na safra de primavera, quando ocorre maior incidência de insetos pragas e, conseqüentemente, danos nos tubérculos de batata no Rio Grande do Sul. As diferentes dosagens e formulações dos inseticidas foram aplicadas no sulco de plantio, utilizando-se clorpirifós (2, 3 e 4 l i.a./ha de Lorsban 480 CE e 2 e 3kg i.a./ha de Lorsban 10 G e como padrão, forate (2kg i.a./ha de Granutox 50G. Os melhores controles ocorreram nos tratamentos com Granutox 50G, na dosagem de 2kg i.a./ha; seguido por Lorsban 10 G, 3 e 2kg i.a./ha e Lorsban 480 CE, 4 e 3l i.a./ha.A field trial was conducted to evaluate the performance of the insecticide chlorpyrifos in two formulations, emulsifiable and granular, for the control of Diabrotica speciosa (Germ. (Coleoptera:Chrysomelidae in potato. The field trial was conducted during the spring season when incidence of insect pests and, consequently, damage are high in Rio Grande do Sul state, Brazil. The different dosages and formulations of insecticides were applied in the planting furrow utilizing chlorpyrifos (2, 3 and 4l i.a./ha of Lorsban 480 CE and 2 and 3kg i.a./ha de Lorsban 10G and the standard phorate (2kg i.a./ha of Granutox 50G. Best field control performances were obtained with Granutox 50G, in the dosage of 2kg i.a./ha; Lorsban 10G, 3 and 2kg i.a./ha, followed by Lorsban 480 CE, 4 and 3l i.a./ha.

  13. Physiologic, morphologic and behavioural responses of Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) related to the consume of different varieties of Phaseolus vulgaris

    International Nuclear Information System (INIS)

    This study was developed aiming to verify physiological, morphological and behavioral responses of two different Zabrotes subfasciatus (Boheman) populations to different beans varieties (Phaseolus vulgaris) (Fabaceae). Female longevity, fertility and oviposition preference site, as well as size and levels of fluctuating asymmetry for males and females were described. Zabrotes subfasciatus displayed physiological plasticity in response to the diet, which was considered an important adaptive ability to maintain the insect generalist habit for food consumption and oviposition sites. The populations studied had different responses to the same treatments, indicating genetic, physiological and behavioral variation on their plastic potential. The Hopkins' principle, which determines the influence of previous female experience in the choice of oviposition sites, was not confirmed. The occurrence of fluctuating asymmetry in males and females was variable, probably as a consequence of genomic factors determining this trait. (author)

  14. A new species of bromeliad-feeding Cephaloleia Chevrolat (Coleoptera, Chrysomelidae, Cassidinae from Costa Rica: evidence from DNA barcodes, larval and adult morphology and insect diets

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    Carlos Garcia-Robledo

    2015-01-01

    Full Text Available The Neotropical genus Cephaloleia Chevrolat (Coleoptera: Chrysomelidae: Cassidinae includes 214 species distributed from the south of Mexico to Argentina. Cephaloleia beetles feed mostly on plants from the order Zingiberales. The interactions between Cephaloleia beetles and their Zingiberales host plants is proposed as one of the oldest and most conservative associations. Here we describe a new species of Cephaloleia (C. kuprewiczae sp. n. that feeds on two species of bromeliads (Pitcairnia arcuata and P. brittoniana, Bromeliaceae: Pitcairnioideae. Cephaloleia kuprewiczae was previously described as Cephaloleia histrionica. This study includes evidence from DNA barcodes (COI, larval and adult morphology and insect diets that separates C. kuprewiczae from C. histrionica as a new species.

  15. Molecular phylogeny of Diabrotica beetles (Coleoptera: Chrysomelidae) inferred from analysis of combined mitochondrial and nuclear DNA sequences.

    Science.gov (United States)

    Clark, T L; Meinke, L J; Foster, J E

    2001-08-01

    The phylogenetic relationships of thirteen Diabrotica (representing virgifera and fucata species groups) and two outgroup Acalymma beetle species (Coleoptera: Chrysomelidae) were inferred from the phylogenetic analysis of a combined data set of 1323 bp of mitochondrial DNA (mtDNA) cytochrome oxidase subunit 1 (COI) and the entire second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA of 362 characters. Species investigated were D. adelpha, D. balteata, D. barberi, D. cristata, D. lemniscata, D. longicornis, D. porracea, D. speciosa, D. undecimpunctata howardi, D. u. undecimpunctata, D. virgifera virgifera, D. v. zeae, D. viridula, and outgroup A. blandulum and A. vittatum. Maximum parsimony (MP), minimum evolution (ME), and maximum likelihood (ML) analyses of combined COI and ITS-2 sequences clearly place species into their traditional morphological species groups with MP and ME analyses resulting in identical topologies. Results generally confer with a prior work based on allozyme data, but within the virgifera species group, D. barberi and D. longicornis strongly resolve as sister taxa as well as monophyletic with the neotropical species, D. viridula, D. cristata and D. lemniscata also resolve as sister taxa. Both relationships are not in congruence with the prior allozyme-based hypothesis. Within the fucata species group, D. speciosa and D. balteata resolve as sister taxa. Results also strongly supported the D. virgifera and D. undecimpunctata subspecies complexes. Our proposed phylogeny provides some insight into current hypotheses regarding distribution status and evolution of various life history traits for Diabrotica. PMID:11520353

  16. Coleoptera families other than Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae. Chapter 8.5

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    Olivier Denux

    2010-07-01

    Full Text Available Here we consider 274 alien Coleoptera species belonging to 41 of the 137 beetle families in Europe (Cerambycidae, Curculionidae sensu lato, Chrysomelidae sensu lato and Coccinelidae are treated separately elsewhere. Among the families we consider asinvaded the European fauna, Acanthocnemidae and Ptilodactylidae represent new arrivals. Many species-rich families have surprisingly few aliens, whereas some relatively minor families such as Dermestidae, Nitidulidae and Anobiidae have a relatively high representation of alien species. Since the start of the 19th century, the number of coleopteran aliens introduced into Europe has continued to increase. Alien species colonizing Europe derive from a wide range of geographic regions as well as ecozones, but the most important source area is Asia. The countries with the largest number of alien species established are France, Germany and Italy. The majority have been introduced accidentally via international transport mechanisms. The most important route for importation is stored products and crops, followed by transport of wood, then horticultural and ornamental plants. Most alien species in these families are found within anthropogenic habitats in Europe. The introduction of invasive alien beetles in these families has had significant economic impacts, particularly as pests of stored foodstuffs, as well as serious ecological impacts. For example, the buprestid species Agrilus planipennis, recently recorded in Russia, is an important potential economic threat which may also impact the biodiversity associated with ash trees.

  17. Inheritance and Fitness Costs of Resistance to Cry3Bb1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Ingber, David A; Gassmann, Aaron J

    2015-10-01

    Transgenic crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely planted to manage pest insects. One of the primary pests targeted by Bt corn in the United States is western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae). Cry3Bb1 corn for management of western corn rootworm was commercialized in 2003, and beginning in 2009, populations of western corn rootworm with field-evolved resistance to Cry3Bb1 corn were found in Iowa. Here we quantify the magnitude, inheritance, and fitness costs of resistance to Cry3Bb1 corn in two strains (Hopkinton and Cresco) derived from field populations that evolved resistance to Cry3Bb1 corn. For Hopkinton, we found evidence for complete resistance to Cry3Bb1 corn and nonrecessive inheritance. Additionally, no fitness costs of Cry3Bb1 resistance were detected for Hopkinton. For Cresco, resistance was incomplete and recessive, and we detected fitness costs affecting developmental rate, survival to adulthood, and fecundity. These results suggest that variation may exist among field populations in both the inheritance and accompanying fitness costs of resistance. To the extent that field populations exhibit nonrecessive inheritance and a lack of fitness cost, this will favor more rapid evolution of resistance than would be expected when resistance is functionally recessive and is accompanied by fitness costs. PMID:26453731

  18. Developmental Biology and Effects of Adult Diet on Consumption, Longevity, and Fecundity of Colaspis crinicornis (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Miwa, Kentaro; Meinke, Lance J

    2015-01-01

    The chrysomelid beetle Colaspis crinicornis Schaeffer (Coleoptera: Chrysomelidae) occurs primarily in the Great Plains region of the United States. Little is known about the biology and ecology of this species, but over the last decade, it has become increasingly common in the corn, Zea mays L., and soybean, Glycine max (L.) Merrill, agroecosystem of southeastern Nebraska. As part of a larger comprehensive project to understand the natural history and pest potential of this species, laboratory experiments were conducted to study the developmental biology, morphological characters of immature stages, and the effect of adult diet on consumption, longevity, and fecundity. Females readily deposited egg clusters in the soil, and percentage egg hatch was high under laboratory conditions. Larvae and pupae were confirmed to be soil-dwelling stages. C. crinicornis has relatively short egg, pupal, and adult stages with the majority of its life cycle spent in the larval stage. Results of choice and no-choice adult feeding experiments indicate that diets of corn or soybean leaves did not significantly affect consumption, longevity, or fecundity of adult C. crinicornis, suggesting that corn and soybean leaves are similarly suitable food sources for adults. The ability to effectively utilize tissues from very different plant families as adult food sources suggests that C. crinicornis is polyphagous in the field. PMID:26106090

  19. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations.

    Science.gov (United States)

    Beaudoin-Ollivier, L; Flori, A; Coffi, A; Cros, D; Glitho, I; Nodichao, L

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect's egg laying and feeding. PMID:26113512

  20. Physiologic, morphologic and behavioural responses of Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) related to the consume of different varieties of Phaseolus vulgaris; Respostas fisiologicas, morfologicas e comportamentais de Zabrotes subfasciatus (Boheman) (Coleoptera: Bruchidae) associadas ao consumo de diferentes variedades de feijao (Phaseolus vulgaris)

    Energy Technology Data Exchange (ETDEWEB)

    Marteleto, Patricia B. [Universidade Federal de Uberlandia (UFU), Umuarama, Uberlandia, MG (Brazil). Inst. de Genetica e Bioquimica. Programa de Pos-Graduacao], e-mail: patriciamarteleto@gmail.com; Lomonaco, Cecilia [Universidade Federal de Uberlandia (UFU), Umuarama, Uberlandia, MG (Brazil). Inst. de Biologia], e-mail: lomonaco@ufu.br; Kerr, Warwick E. [Universidade Federal de Uberlandia (UFU), Umuarama, Uberlandia, MG (Brazil). Dept. de Ciencias Agronomicas], e-mail: kerr@ufu.br

    2009-03-15

    This study was developed aiming to verify physiological, morphological and behavioral responses of two different Zabrotes subfasciatus (Boheman) populations to different beans varieties (Phaseolus vulgaris) (Fabaceae). Female longevity, fertility and oviposition preference site, as well as size and levels of fluctuating asymmetry for males and females were described. Zabrotes subfasciatus displayed physiological plasticity in response to the diet, which was considered an important adaptive ability to maintain the insect generalist habit for food consumption and oviposition sites. The populations studied had different responses to the same treatments, indicating genetic, physiological and behavioral variation on their plastic potential. The Hopkins' principle, which determines the influence of previous female experience in the choice of oviposition sites, was not confirmed. The occurrence of fluctuating asymmetry in males and females was variable, probably as a consequence of genomic factors determining this trait. (author)

  1. Flea beetles (Coleoptera: Chrysomelidae: Alticinae collected by malaise trap method in Gölcük Natural Park (Isparta, Turkey, with a new record for Turkish fauna

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    Aslan Gül E.

    2012-01-01

    Full Text Available This study is based on Alticinae (Coleoptera: Chrysomelidae material collected by Malaise trapping which is different from other standardized collecting methods. A total of 19 flea beetle species belonging to 6 genera were collected from Gölcük Natural Park, Isparta (Turkey during 2009. The species are listed in a table together with distributional data in Turkey. Among them, Longitarsus curtus (Allard, 1860 is recorded for the first time in Turkey. L. monticola Kutschera, 1863 and L. curtus are recently separated synonyms and thus all data referring to the distribution of both species are currently important. Hence, the zoogeographical distribution of the new record is reviewed with some remarks; habitus and genitalia are illustrated.

  2. Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae

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    Mara Cristina de Almeida

    2006-01-01

    Full Text Available Species of the subtribe Oedionychina not only have a highly uniform diploid number of 2n = 22 (20+X+y but have the karyotypic peculiarity of possessing extremely large sex chromosomes. We analyzed Paranaita opima embryos and gonadal cells to determine their diploid number, chromosomal morphology, type of sex determination system, constitutive heterochromatin pattern and which chromosomes bear nucleolus organizer regions (NORs. The diploid number of P. opima was 2n = 22 (20+XY/XX with all chromosomes being metacentric. Chromosome pair 6 showed an interstitial secondary constriction on the short arm. The C-banding technique revealed centromeric constitutive heterochromatin in all chromosomes, which, in pair 6, extended up to the secondary constriction of the short arm, additional C-bands also being present on the Y chromosome. Silver nitrate nucleolar organizer region (Ag-NOR staining showed NORs on the secondary constriction of pair 6. Fluorochrome analysis with chromomycin A3 (CMA3, 4'-6-diamidino-2-phenylindole (DAPI and the distamycin A (DA counterstain showed that the short arm of chromosome pair 6 exhibited a GC-rich block extending from the proximal to the median region, including part of the secondary constriction. The same techniques also showed AT-rich blocks at the centromeric region of all chromosomes and at the terminal region of the short arm of pair 6. The basic karyotype characteristics and C band pattern of P. opima are similar to those described for other species in the subtribe Oedionychina. The pattern of autosomal NORs observed in P. opima corresponds to that registered in the majority of the Chrysomelidae species.

  3. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    Science.gov (United States)

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect’s egg laying and feeding. RÉSUMÉ. La comestibilité de différents matériels végétaux d’Elaeis sp. présents au Bénin est testée pour Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), important ravageur du palmier à huile en Afrique. Des expérimentations en manchons ont permis de déterminer les capacités d’oviposition des femelles et les taux de mortalité des différents stades de d

  4. Resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae Resistence of potatoes genotypes (Solanum spp. to Diabrotica speciosa (Germar,1824 (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Fernando Mesquita Lara

    2000-12-01

    Full Text Available Avaliou-se a resistência de genótipos de batata (Solanum spp. a Diabrotica speciosa (Germar,1824 em condições de campo e laboratório. O plantio de campo foi efetuado em julho de 1997, na FCAV/ UNESP, Campus de Jaboticabal, utilizando-se os seguintes genótipos: Achat, NYL 235-4, 288.759-16, 288. 771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. Avaliaram-se o consumo de área foliar em levantamentos realizados aos 70 e 90 dias após plantio e os danos ocasionados pelas larvas aos tubérculos, após a colheita. Em laboratório, foram realizados testes de preferência para alimentação com plantas de 40 dias (teste sem chance de escolha, envolvendo os nove genótipos presentes no campo, e com plantas de 47 e 68 dias (com e sem chance de escolha, avaliando-se cinco genótipos. Constatou-se, no ensaio de campo, que os genótipos 288.759-16, 288.776-3, 288.776-6, 288.787-5 e 288.814-7 foram menos consumidos por adultos de D. speciosa que a cultivar Achat, porém, em laboratório, 288.759-16 e 288.776-6 não rev elaram essa característica. O consumo em NYL 235-4 e 288.801-8 foi intermediário, não diferindo dos demais. NYL 235-4 e 288.801-8 foram resistentes às larvas, apresentando, em média, menos que 3 furos por tubérculo. O genótipo 288.776-3 destacou-se como resistente a larvas e adultos de D. speciosa, sendo a resistência a adultos do tipo não-preferência para alimentação (antixenose.The resistance of potato genotypes to Diabroticaspeciosa (Germar,1824 (Coleoptera: Chrysomelidae, was evaluated under field and laboratory conditions. The field trial was set up in the experimental farm of FCAV - UNESP, Jaboticabal, SP, and the following genotypes were compared: Achat, NYL 235-4, 288.759-16, 288.771-6, 288.776-3, 288.776-6, 288.787-5, 288.801-8 e 288.814-7. The leaf area consumption by adults was evaluated at 70 and 90 days after planting, and, after harvest, larval damage to tubers (number of perforations was assessed

  5. Plant vigor metrics determine spatio-temporal distribution dynamics of Oulema melanopus (Coleoptera: Chrysomelidae) and its larval parasitoid, Tetrastichus julis (Hymenoptera: Eulophidae).

    Science.gov (United States)

    Kher, S V; Dosdall, L M; Cárcamo, H A

    2014-10-01

    The cereal leaf beetle, Oulema melanopus (L.) (Coleoptera: Chrysomelidae), is a new invasive insect pest of oat, wheat, and barley in western Canada. Biological control with its principal larval parasitoid, Tetrastichus julis Walker (Hymenoptera: Eulophidae), is the primary management strategy. However, to implement control successfully, a thorough understanding of the spatio-temporal dynamics of the interactions between these two species is important. We examined the nature of spatial associations and distribution dynamics of O. melanopus and T. julis with reference to host plant nutrients and plant vigor traits using Spatial Analysis by Distance Indices. A grid design was used to understand spatial associations between O. melanopus and T. julis. Distributions of O. melanopus and T. julis indicated the presence of significant patches and gaps. Plant nutrient availability and plant vigor varied across the grid in all study years. On a spatial scale, O. melanopus and T. julis represented a tightly coupled system demonstrating the strong density-dependent nature of parasitoid dispersal. Among the factors examined, plant vigor traits significantly influenced field distributions of both O. melanopus and T. julis. Areas across grids with high plant density, greater plant height, and high availability of plant leaves indicated higher establishment of O. melanopus larvae, consequently exhibiting bottom-up effects on T. julis distributions. Maintenance of uniform plant vigor can be a critical aspect in mitigating yield losses from O. melanopus infestation. PMID:25259693

  6. Concentration-dependent effects of GABA on insensitivity to fipronil in the A2'S mutant RDL GABA receptor from fipronil-resistant Oulema oryzae (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Nakao, Toshifumi; Naoi, Atsuko; Hama, Masako; Kawahara, Nobuyuki; Hirase, Kangetsu

    2012-10-01

    The beetle Oulema oryzae Kuwayama (Coleoptera: Chrysomelidae), an important pest of rice, has developed fipronil resistance in Japan. Molecular cloning and sequence analysis of O. oryzae RDL gamma-aminobutyric acid (GABA) receptor subunit (OO-RDL) genes from fipronil-susceptible and -resistant O. oryzae identified the A2'S mutation (index number for the M2 membrane-spanning region). To investigate the effect of the A2'S mutation on fipronil resistance, we stably expressed the wild-type and mutant OO-RDL homomers in Drosophila Mel-2 cells. A membrane potential assay exhibited that the IC50 values of fipronil for inhibition of the response to EC80 GABA of the wild-type and A2'S mutant OO-RDL homomers were 0.09 microM and 0.11 microM, respectively. However, the IC50 values of fipronil for inhibition of the response to EC95 GABA of the wild-type and A2'S mutant OO-RDL homomers were 0.11 microM and approximately equal to 5 microM, respectively. These results suggest that the GABA concentration is an important factor affecting fipronil resistance in O. oryzae carrying the A2'S mutation in OO-RDL. PMID:23156177

  7. Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and Diabrotica barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data.

    Science.gov (United States)

    Coates, Brad S

    2014-06-01

    Complete mitochondrial genomes for two corn rootworm species, Diabrotica virgifera virgifera (16,747 bp) and Diabrotica barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and 22 tRNA and 2 rRNA sequences were in typical of insect mitochondrial genomes. Non-standard nad4 and cox3 stop codons were composed of single T nucleotides and likely completed by adenylation, and atypical TTT start codons was predicted for both D. v. virgifera and D. barberinad1 genes. The D. v. virgifera and D. barberi haplotypes showed 819 variable nucleotide positions within PCG regions (7.36% divergence), which suggest that speciation may have occurred ~3.68 million years ago assuming a linear rate of short-term substitution. Phylogenetic analyses of Coleopteran MtD genome show clustering based on family level, and may have the capacity to resolve the evolutionary history within this Order of insects. PMID:24657060

  8. Chrysomelid males with enlarged mandibles: three new species and a review of occurrence in the family (Coleoptera: Chrysomelidae).

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    Reid, C A M; Beatson, M

    2013-01-01

    Three new species of Chrysomelidae with extraordinary extensions of the male mandibles are described: Scaphodius drehu sp. nov. and S. ferox sp. nov. (Cryptocephalinae), from New Caledonia, and Chaloenus gajah sp. nov. (Galerucinae), from Borneo. Designation of the type species of Chaloenus Westwood, 1861, is clarified. Synonymy of Scaphodius Chapuis, 1874, with Nyetra Baly, 1877, is supported. Four species of Ditropidus Erichson, 1842, described from New Caledonia, but hitherto regarded as nomina nuda, are shown to be available and are placed in Scaphodius: S. aeneus (Fauvel, 1907), comb. nov., S. nitidus (Fauvel, 1907) comb. nov., S. striolatus (Fauvel, 1907) comb. nov., S. sulcatus (Fauvel, 1907) comb. nov. Ditropidus opacicollis Fauvel, 1907, is also transferred to Scaphodius, as S. opacicollis (Fauvel) comb. nov. The genus Ditropidus does not occur on New Caledonia. Male mandible enlargment in the Chrysomelidae is reviewed: it is common in Cryptocephalinae, but otherwise restricted to a few species of Chrysomelinae, Eumolpinae and Galerucinae. Possible reasons for its distribution in the Chrysomelidae are discussed. PMID:26131467

  9. Comportamento de larvas de Diabrotica speciosa (Coleoptera: Chrysomelidae em resposta ao CO2 e a plântulas de espécies cultivadas Behavioral responses of Diabrotica speciosa (Coleoptera: Chrysomelidae larvae to CO2 and seedlings of host plants

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    Tânia Pereira

    2005-10-01

    Full Text Available As respostas de larvas de Diabrotica speciosa (Germar (Coleoptera: Chrysomelidae ao CO2 e o comportamento destas larvas quando expostas a plantas hospedeiras e não hospedeiras foram estudadas em laboratório. Larvas de primeiro ínstar de D. speciosa foram utilizadas. Num primeiro bioensaio, as larvas preferiram o tratamento com CO2, que foi gerado a partir da reação de bicarbonato de potássio com ácido acético em oposição a água destilada. Quando se observou o comportamento das larvas em relação a plantas hospedeiras, verificou-se que ocorreu um número significativamente maior de "mudanças de direção" em milho e milho pipoca do que em feijão, soja, trigo e sorgo. Este parâmetro foi inferior no sorgo em relação a todos os outros tratamentos. Valores similares foram verificados em milho pipoca e milho (26,2 e 24,2, respectivamente. Em relação a distância percorrida, foram verificadas maiores distâncias na aveia em comparação com milho pipoca, milho, feijão e sorgo. Após o contato das larvas com sorgo, elas não apresentaram comportamento de busca, pois a larva caía da plântula ou não se movia .Responses of Diabrotica speciosa (Germar (Coleoptera: Chrysomelidae larvae to CO2 and their behavior when exposed to host and non host plants were studied in the laboratory. First instar larvae of D. speciosa were used. In a first bioassay, larvae preferred the treatment with CO2, from the reaction of the potassium bicarbonate and acetic acid, in opposition to distillated water. When the behavior of the larvae was observed in response to host plants, a significant higher number of turns was found in corn and popcorn than in common beans, soybean, wheat and sorghum. Sorghum differed from all other treatments with a lower number of turns. Similar values were found in popcorn and corn. The greater distances ranged were found on oats in comparison to pop corn, corn, common beans and sorghum. After contact with sorghum seedlings

  10. Mortality and impaired development of Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae induced by extract of sangra d’água Croton urucurana Baill (Euphorbiaceae.

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    Gabriel dos Santos Carvalho

    2014-09-01

    Full Text Available Phaseolus vulgaris L., the green bean, is an important source of protein and minerals in the diet of the population. But its productivity is affected by the attack of numerous pests, from emergence to storage, Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae being the major pest of stored beans. In the search for alternatives to chemical control of this insect and considering the potential biological activity of plant secondary metabolites, this study was conducted in order to evaluate the toxicity of the extract of Croton urucurana Baill (Euphorbiaceae on Z. subfasciatus. The insects were subjected to concentration-mortality bioassays with determination of LC50. We also determined the effect of the extract on the number of insects that emerged after a period of confinement, topical testing, vaporization and instantaneous rate of population growth, grain consumption and body mass of insects fed grain impregnated with extract of C. urucurana. The LC50 was estimated at 2560 ppm; mortality increased as a function of the exposure period. The grain treated with the extract at the same concentration as in the topical tests more significantly affected survival of Z. subfasciatus. Behavioral characteristics, survival and emergence varied in all treatments; differences were significant. Higher percentage mortality occurred in vaporization tests of the flower extract. The instantaneous rate of growth, grain consumption and body mass of insects were significantly reduced in the treatment with beans exposed to 0.01 g/mL of C. urucurana extract. Thus it can be concluded that the extract of C. urucurana interferes with the survival and development of Z. subfasciatus; giving continuity to this research a phytochemical investigation of the plant is being undertaken, guided by bioassays to elucidate the compounds responsible for the deleterious action in insects.

  11. Incidence, Spread and Mechanisms of Pyrethroid Resistance in European Populations of the Cabbage Stem Flea Beetle, Psylliodes chrysocephala L. (Coleoptera: Chrysomelidae)

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    Højland, Dorte H.; Nauen, Ralf; Foster, Stephen P.; Williamson, Martin S.; Kristensen, Michael

    2015-01-01

    Background Cabbage stem flea beetle (CSFB), Psylliodes chrysocephala L. (Coleoptera: Chrysomelidae) is a major early season pest of oilseed rape throughout Europe. Pyrethroids have been used for controlling this pest by foliar application, but in recent years control failures have occurred, particularly in Germany due to the evolution of knock-down resistance (kdr). The purpose of this study was to investigate the incidence and spread of pyrethroid resistance in CSFB collected in Germany, Denmark and the United Kingdom during 2014. The level of pyrethroid resistance was measured in adult vial tests and linked to the presence of kdr genotypes. Results Although kdr (L1014F) genotypes are present in all three countries, marked differences in pyrethroid efficacy were found in adult vial tests. Whereas Danish CSFB samples were in general susceptible to recommended label rates, those collected in the UK mostly resist such rates to some extent. Moderately resistant and susceptible samples were found in Germany. Interestingly, some of the resistant samples from the UK did not carry the kdr allele, which is in contrast to German CSFB. Pre-treatment with PBO, prior to exposure to λ-cyhalothrin suggested involvement of metabolic resistance in UK samples. Conclusion Danish samples were mostly susceptible with very low resistance ratios, while most other samples showed reduced sensitivity in varying degrees. Likewise, there was a clear difference in the presence of the kdr mutation between the three countries. In the UK, the presence of kdr genotypes did not always correlate well with resistant phenotypes. This appears to be primarily conferred by a yet undisclosed, metabolic-based mechanism. Nevertheless our survey disclosed an alarming trend concerning the incidence and spread of CSFB resistance to pyrethroids, which is likely to have negative impacts on oilseed production in affected regions due to the lack of alternative modes of action for resistance management purposes

  12. Faunistic patterns of leaf beetles (Coleoptera, Chrysomelidae) within elevational and temporal gradients in Sierra de San Carlos, Mexico.

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    Sánchez-Reyes, Uriel Jeshua; Niño-Maldonado, Santiago; Barrientos-Lozano, Ludivina; Clark, Shawn M; Jones, Robert W

    2016-01-01

    The study of biodiversity of Chrysomelidae in Mexico and its variation within ecological gradients has increased recently, although important areas in the country remain to be explored. We conducted a faunistic inventory and analyzed the elevational and temporal variation of leaf beetle communities in the Sierra de San Carlos, in the state of Tamaulipas, in northeastern Mexico. This is an area with high to extreme priority for conservation, and due to its insular geographical position and to the vegetational communities present, it must be considered as a sky island. We selected seven sample sites distributed in different elevations within three localities, and comprising different vegetational communities. At each site, we randomly delimited 12 sample plots of 400 m(2) where sampling was conducted by entomological sweep netting and collecting directly by hand. Sampling was conducted monthly at each plot, for a total of 1,008 samples between February 2013 and January 2014. By the end of the study, we had obtained a total of 3,081 specimens belonging to six subfamilies, 65 genera, and 113 species, with Trichaltica scabricula (Crotch, 1873) being recorded for first time in Mexico. Species richness was less than the values observed at other studies conducted in the same region, which is attributed to differences in the number of plant species and to the insular location of Sierra de San Carlos; however, the higher diversity values suggest a higher quality of natural resources and vegetational communities. No consistent pattern of leaf beetle communities was correlated with elevation, although higher values of species richness and diversity were obtained at the highest elevation site. The seasonal gradient showed that the rainy season is most favorable for leaf beetle communities. We found that species composition was different between sites and months, and also that there exists a significant association between the abundance obtained at each site and particular

  13. Observaciones sobre el gualapán (Coleoptera: Chrysomelidae: Hispinae y otras limitantes entomológicas en cultivos de chontaduro en el Bajo Anchicayá

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    Alarcón Andrés

    2005-06-01

    Full Text Available Se realizaron observaciones y colectas sistemáticas desde enero a diciembre de 1996, en compañía de agricultores del Bajo Anchicayá, Valle (60-140 msnm, Bosque pluvial tropical, 24º C, precipitación mayor a 4000 mm. Alurnus sp, localmente llamado “gualapánâ€? o “llagaâ€?, puede afectar del 50 al 100% del follaje joven de la palma y en asocio circunstancial con curculionidos barrenadores del estipe conformar complejos letales (Rhynchophorus palmarum L., Dynamis borassi Fabr, Rhinostomus barbirostris Fabr., Metamasius hemipterus Fabr., y M. dasyurus Champion. Se aportan observaciones sobre la etiología, aspectos autoecológicos de la plaga y de los complejos entomológicos. Las limitaciones edáficas, desbalances nutricionales, sumados a prácticas agrícolas inadecuadas (escalamiento de palmas y uso descuidado de plaguicidas contribuyen significativamente con la problemática entomológica, se propone un manejo con enfoque ecológico integral. ABSTRACT Observations on “Gualapanâ€? (Coleoptera: Chrysomelidae: Hispinae and others entomological limitations chontaduro’s crops located in Bajo Anchicaya, Valle, Colombia.We did several observations and collect from January to December of 1996, with the help of local farmers of Bajo Anchicaya, Valle (altitude of 60 to 140 m.a.s.l., it’s a tropical rainforest, temperature 24 C, and rainfall is 4000 millimeters. Alurnus sp, named “Gualapanâ€? or “Soreâ€? by the local people, may affect from 50 to 100% of the palm’s foliage, and in rare cases with the help of curculionids could be a lethal partnership (Rhynchophorus palmarum L., Dynamis borassi Fabr, Rhinostomus barbirostris Fabr., Metamasius hemipterus Fabr., y M. dasyurus Champion. The edafologics limitants, nutritional imbalances, in conjunction with inappropriate agricultural practices (e.g.: escalade of palms trees and inappropriate use of pesticides contribute enormously

  14. Identification and Comparison of Candidate Olfactory Genes in the Olfactory and Non-Olfactory Organs of Elm Pest Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae Based on Transcriptome Analysis.

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    Yinliang Wang

    Full Text Available The leaf beetle Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae is a predominant forest pest that causes substantial damage to the lumber industry and city management. However, no effective and environmentally friendly chemical method has been discovered to control this pest. Until recently, the molecular basis of the olfactory system in A. quadriimpressum was completely unknown. In this study, antennae and leg transcriptomes were analyzed and compared using deep sequencing data to identify the olfactory genes in A. quadriimpressum. Moreover, the expression profiles of both male and female candidate olfactory genes were analyzed and validated by bioinformatics, motif analysis, homology analysis, semi-quantitative RT-PCR and RT-qPCR experiments in antennal and non-olfactory organs to explore the candidate olfactory genes that might play key roles in the life cycle of A. quadriimpressum. As a result, approximately 102.9 million and 97.3 million clean reads were obtained from the libraries created from the antennas and legs, respectively. Annotation led to 34344 Unigenes, which were matched to known proteins. Annotation data revealed that the number of genes in antenna with binding functions and receptor activity was greater than that of legs. Furthermore, many pathway genes were differentially expressed in the two organs. Sixteen candidate odorant binding proteins (OBPs, 10 chemosensory proteins (CSPs, 34 odorant receptors (ORs, 20 inotropic receptors [1] and 2 sensory neuron membrane proteins (SNMPs and their isoforms were identified. Additionally, 15 OBPs, 9 CSPs, 18 ORs, 6 IRs and 2 SNMPs were predicted to be complete ORFs. Using RT-PCR, RT-qPCR and homology analysis, AquaOBP1/2/4/7/C1/C6, AquaCSP3/9, AquaOR8/9/10/14/15/18/20/26/29/33, AquaIR8a/13/25a showed olfactory-specific expression, indicating that these genes might play a key role in olfaction-related behaviors in A. quadriimpressum such as foraging and seeking. AquaOBP4/C5, Aqua

  15. Oviposition behavior of wild Zabrotes subfasciatus (Coleoptera, Chrysomelidae females deprived of the host Phaseolus vulgaris (Fabaceae Comportamento de ovoposição de fêmeas selvagens de Zabrotes subfasciatus (Coleoptera, Chrysomelidae privadas do hospedeiro Phaseolus vulgaris (Fabaceae

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    Luiza A. A Sperandio

    2009-12-01

    Full Text Available The insects oviposition behavior is fundamental to study population dynamics, life history evolution, insect-plant and parasitoid-host interactions. Zabrotes subfasciatus (Boheman, 1833 females oviposition behavior in the presence and absence of a host is unknown. The main objective of this study was to describe in detail the oviposition behavior of host deprived or non-deprived females, and observe how the several situations of deprivation (days without host influence oviposition. Six groups were assembled, three deprived of the host (for 2, 5 and 8 days and three control groups (with host, each containing one newly-emerged couple (0-24h of wild Z. subfasciatus, The non-deprived (control groups received the hosts every day (5 bean seeds Phaseolus vulgaris (Fabaceae and the others were deprived for 2, 5 and 8 days, respectively. For each group 12 repetitions were made. Consequently, 12 couples were host deprived during two days, 12 couples were host deprived during five days and 12 couples were host deprived during eight days. When the seeds of the deprived groups were added the experiments started. There was a control group for each deprived group. The experiments and the insects were maintained at constant temperature 29 ± 2ºC and 70-80% relative humidity. At 15 minutes interval, the number of times the females manifested the different categories of behavior was observed (frequency. The behavior categories were: rest inside the box, locomotion, resource exploration (seeds, copulation and oviposition. The deprived females stayed most of the time in contact with the host to carry out oviposition, while the non-deprived (control females spent most of the time at rest. This was observed in all the deprivation times. The results show that host deprivation influences the oviposition behavior of the studied species and also shows the flexibility in the oviposition strategies that these females present when the environment changes (absence and

  16. Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae)

    International Nuclear Information System (INIS)

    Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Huefnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae). The occurrence of Diabrotica speciosa (Germar, 1824) and Agrotis ipsilon (Huefnagel, 1767) on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum quantity of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha-1 of N + K with 27.03% at P-1, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensitized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing. (author)

  17. Análise comparativa dos surtos e danos causados pelos besouros desfolhadores Costalimaita ferruginea (Fabricius, 1801 e Costalimaita lurida (Lefévre, 1891 (Coleoptera: Chrysomelidae em plantios de eucalipto

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    Reginaldo Gonçalves Mafia

    2014-10-01

    Full Text Available Os besouros desfolhadores Costalimaita ferruginea (Fabricius, 1801 e Costalimaita lurida (Lefévre, 1891 (Coleoptera: Chrysomelidae são pragas importantes da cultura do eucalipto. A biologia e comportamento daninho de C. ferruginea já foram caracterizados. Todavia, não existem estudos sobre o potencial de C. lurida como praga da cultura. Assim, o objetivo deste trabalho foi avaliar, de forma comparativa, os surtos e as injúrias causadas por C. ferruginea e C. lurida em plantios de eucalipto. Para isso, 202,8 mil ha de plantios clonais de eucalipto, nos Estados do Espírito Santo, da Bahia e de Minas Gerais, foram monitorados no período crítico de ocorrência (quente e chuvoso. Os surtos detectados foram caracterizados, determinando-se, por meio de amostragens, a incidência, a severidade e as populações absoluta e relativa do inseto por planta atacada. De forma geral, o comportamento daninho de C. lurida foi similar ao de C. ferruginea, em termos de época de ocorrência e tipo de injúrias causadas. Todavia, C. lurida apresentou maiores picos populacionais, em termos absolutos (insetos por planta e relativos (razão entre o número de insetos e altura das plantas atacadas, e atingiu maior proporção de área, podendo ser considerado mais daninho que C. ferruginea. O besouro C. lurida deve ser monitorado considerando os mesmos critérios. Além disso, os surtos desta nova praga poderão ser avaliados quanto ao nível crítico para controle, empregando-se as mesmas premissas adotadas para C. ferruginea. Adicionalmente aos resultados, registrou-se, pela primeira vez, a ocorrência de C. lurida no Estado da Bahia, em plantas de araçá (Psidium guineense, Swartz (Myrtaceae.

  18. Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays.

    Science.gov (United States)

    Gassmann, Aaron J; Shrestha, Ram B; Jakka, Siva R K; Dunbar, Mike W; Clifton, Eric H; Paolino, Aubrey R; Ingber, David A; French, B Wade; Masloski, Kenneth E; Dounda, John W; St Clair, Coy R

    2016-08-01

    Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States, and recent management of western corn rootworm has included planting of Bt corn. Beginning in 2009, western corn rootworm populations with resistance to Cry3Bb1 corn and mCry3A corn were found in Iowa and elsewhere. To date, western corn rootworm populations have remained susceptible to corn producing Bt toxin Cry34/35Ab1. In this study, we used single-plant bioassays to test field populations of western corn rootworm for resistance to Cry34/35Ab1 corn, Cry3Bb1 corn, and mCry3A corn. Bioassays included nine rootworm populations collected from fields where severe injury to Bt corn had been observed and six control populations that had never been exposed to Bt corn. We found incomplete resistance to Cry34/35Ab1 corn among field populations collected from fields where severe injury to corn producing Cry34/35Ab1, either singly or as a pyramid, had been observed. Additionally, resistance to Cry3Bb1 corn and mCry3A corn was found among the majority of populations tested. These first cases of resistance to Cry34/35Ab1 corn, and the presence of resistance to multiple Bt toxins by western corn rootworm, highlight the potential vulnerability of Bt corn to the evolution of resistance by western corn rootworm. The use of more diversified management practices, in addition to insect resistance management, likely will be essential to sustain the viability of Bt corn for management of western corn rootworm. PMID:27329619

  19. Impacto dos nutrientes N e K e de açúcares solúveis sobre populações de Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae e Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae na cultura da batata, Solanum tuberosum L. (Solanaceae Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae and Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae populations in potato crops, Solanum tuberosum L. (Solanaceae

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    Edson Henrique de Azeredo

    2004-03-01

    Full Text Available Foi estudada a ocorrência de Diabrotica speciosa (Germar, 1824 (Coleoptera, Chrysomelidae e de Agrotis ipsilon (Hüfnagel, 1767 (Lepidoptera, Noctuidae em plantas de batata, cultivares Achat e Monalisa, influenciadas por dosagens de nitrogênio e potássio, e teor mínimo de açúcares solúveis. Os seguintes parâmetros foram avaliados: concentração de nutrientes minerais e açúcar em folha verde, folha senescente, folha em abcisão, haste, tubérculo e planta total usando extratos de infusão em etanol 80%. A maior infestação por larvas de D. speciosa foi na cultivar Monalisa a 150 kg.ha-1 de N + K com 27,03% a PThe occurrence of Diabrotica speciosa (Germar, 1824 and Agrotis ipsilon (Hüfnagel, 1767 on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum theor of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha-1 of N + K with 27.03% at P< 0,05. It was observed that the effect of the dosage of N + K in the increment of the concentration of soluble sugars increased the damages in the tubercles and stems by A. ipsilon. The infestation by these species increased to 58.82% on the Monalisa cultivar, when the nitrogen dosage increased from zero to 150 kg.ha-1, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensibilized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing.

  20. Diversidade e sazonalidade de crisomelídeos (Coleoptera: Chrysomelidae em pomar, no município de Ponta Grossa, Paraná, Brasil

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    Julianne Milléo

    2013-06-01

    Full Text Available Este trabalho teve como objetivo levantar informações sobre os padrões de composição faunística, estrutura da comunidade e sazonalidade de Chrysomelidae num pomar localizado na região dos Campos Gerais do Paraná. Durante cerca de dois anos de amostragem, foram coletados 3.661 coleópteros e, destes, 1.103 crisomelídeos, representando 30,1% dos besouros capturados. Eumolpinae e Galerucinae foram as duas subfamílias que apresentaram maior abundância e riqueza de espécies, sendo que as oito mais representativas no pomar foram: Eumolpinae sp. 7 e Eumolpinae sp. 15, Diabrotica speciosa, Iphimeis dives, Spintherophyta semiaurata, Colaspis sp. 1 e Colaspis sp. 2 e Syphrea sp. 1, representando 91,7% dos indivíduos coletados. Em relação às árvores frutíferas, observou-se que o maior número de insetos foi coletado em laranjeira e tangerineira; a laranjeira teve a maior riqueza de espécies, e o caquizeiro apresentou o maior índice de diversidade. A redução considerável de crisomelídeos no segundo ciclo anual demonstra que a família tem uma oscilação temporal acentuada. A primavera foi a estação de maior abundância nos dois anos de coleta, e os fatores meteorológicos não apresentaram correlação com a abundância de crisomelídeos.

  1. Beetle and plant arrow poisons of the Ju|’hoan and Hai||om San peoples of Namibia (Insecta, Coleoptera, Chrysomelidae; Plantae, Anacardiaceae, Apocynaceae, Burseraceae)

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    Chaboo, Caroline S.; Biesele, Megan; Hitchcock, Robert K.; Weeks, Andrea

    2016-01-01

    Abstract The use of archery to hunt appears relatively late in human history. It is poorly understood but the application of poisons to arrows to increase lethality must have occurred shortly after developing bow hunting methods; these early multi-stage transitions represent cognitive shifts in human evolution. This paper is a synthesis of widely-scattered literature in anthropology, entomology, and chemistry, dealing with San (“Bushmen”) arrow poisons. The term San (or Khoisan) covers many indigenous groups using so-called ‘click languages’ in southern Africa. Beetles are used for arrow poison by at least eight San groups and one non-San group. Fieldwork and interviews with Ju|’hoan and Hai||om hunters in Namibia revealed major differences in the nature and preparation of arrow poisons, bow and arrow construction, and poison antidote. Ju|’hoan hunters use leaf-beetle larvae of Diamphidia Gerstaecker and Polyclada Chevrolat (Chrysomelidae: Galerucinae: Alticini) collected from soil around the host plants Commiphora africana (A. Rich.) Engl. and Commiphora angolensis Engl. (Burseracaeae). In the Nyae Nyae area of Namibia, Ju|’hoan hunters use larvae of Diamphidia nigroornata Ståhl. Larvae and adults live above-ground on the plants and eat leaves, but the San collect the underground cocoons to extract the mature larvae. Larval hemolymph is mixed with saliva and applied to arrows. Hai||om hunters boil the milky plant sap of Adenium bohemianum Schinz (Apocynaceae) to reduce it to a thick paste that is applied to their arrows. The socio-cultural, historical, and ecological contexts of the various San groups may determine differences in the sources and preparation of poisons, bow and arrow technology, hunting behaviors, poison potency, and perhaps antidotes. PMID:27006594

  2. Beetle and plant arrow poisons of the Ju|'hoan and Hai||om San peoples of Namibia (Insecta, Coleoptera, Chrysomelidae; Plantae, Anacardiaceae, Apocynaceae, Burseraceae).

    Science.gov (United States)

    Chaboo, Caroline S; Biesele, Megan; Hitchcock, Robert K; Weeks, Andrea

    2016-01-01

    The use of archery to hunt appears relatively late in human history. It is poorly understood but the application of poisons to arrows to increase lethality must have occurred shortly after developing bow hunting methods; these early multi-stage transitions represent cognitive shifts in human evolution. This paper is a synthesis of widely-scattered literature in anthropology, entomology, and chemistry, dealing with San ("Bushmen") arrow poisons. The term San (or Khoisan) covers many indigenous groups using so-called 'click languages' in southern Africa. Beetles are used for arrow poison by at least eight San groups and one non-San group. Fieldwork and interviews with Ju|'hoan and Hai||om hunters in Namibia revealed major differences in the nature and preparation of arrow poisons, bow and arrow construction, and poison antidote. Ju|'hoan hunters use leaf-beetle larvae of Diamphidia Gerstaecker and Polyclada Chevrolat (Chrysomelidae: Galerucinae: Alticini) collected from soil around the host plants Commiphora africana (A. Rich.) Engl. and Commiphora angolensis Engl. (Burseracaeae). In the Nyae Nyae area of Namibia, Ju|'hoan hunters use larvae of Diamphidia nigroornata Ståhl. Larvae and adults live above-ground on the plants and eat leaves, but the San collect the underground cocoons to extract the mature larvae. Larval hemolymph is mixed with saliva and applied to arrows. Hai||om hunters boil the milky plant sap of Adenium bohemianum Schinz (Apocynaceae) to reduce it to a thick paste that is applied to their arrows. The socio-cultural, historical, and ecological contexts of the various San groups may determine differences in the sources and preparation of poisons, bow and arrow technology, hunting behaviors, poison potency, and perhaps antidotes. PMID:27006594

  3. Chaves para a identificação dos principais Coleoptera (Insecta) associados com produtos armazenados Keys for the identification of Coleoptera (Insecta) associated with stored products

    OpenAIRE

    Paulo Roberto Valle da Silva Pereira; Lúcia Massutti de Almeida

    2001-01-01

    An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792), Stegobium paniceum (Linnaeus, 1761)], Bruchidae [Acanthoscelides obtectus (Say, 1831), Zabrotes subfasciatus (Boheman, 1833)], Curculionidae [Sitophilus oryzae (Linnaeus, 1763), S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832), Cathartus quadricollis (Guérin, 1892), Oryzaephilus me...

  4. Adult Diapause in Coleoptera

    Directory of Open Access Journals (Sweden)

    Ivo Hodek

    2012-01-01

    Full Text Available Recent studies dealing with adult (reproductive diapause in the Coleoptera are reviewed, as a kind of supplement to the classic compendia. In the first two sections, the general characteristics of adult diapause are described and principal terms explained. Original articles dealing with 19 species from nine coleopteran families (Coccinellidae, Chrysomelidae, Bruchidae, Curculionidae, Carabidae, Silphidae, Scolytidae, Scarabaeidae, and Endomychidae are reviewed. Finally attempts are made at generalisations from the papers reviewed, and hypotheses on diapause evolution are inferred. A polyphenic character of diapause is a prominent feature in C. septempunctata and L. decemlineata, but has been found also in other Coleoptera and in insects generally and often generates voltinism heterogeneity within populations.

  5. Mechanisms of karyotype differentiation in Cassidinae sensu lato (Coleoptera, Polyphaga, Chrysomelidae) based on seven species of the Brazilian fauna and an overview of the cytogenetic data.

    Science.gov (United States)

    de Julio, Milena; Fernandes, Flávia Rodrigues; Costa, Cleide; Almeida, Mara Cristina; Cella, Doralice Maria

    2010-01-01

    Among the subfamilies of Chrysomelidae, Cassidinae sensu lato (s.l.) includes 6000 species distributed in 43 tribes. Approximately 100 of these species were cytogenetically analyzed and most of them presented 2n=18=16+Xy(p), which was smaller than 2n=20=18+Xy(p) considered basal for Polyphaga. However, some groups of species presented maintenance of the basal diploid number and others showed increase in this number. Certain species of the latter group also exhibited variation in the type of sex chromosome system (SCS). Considering the recent taxonomic revision accomplished for the Cassidinae s.l. species, the existence of phylogenetic relationship for some species of this subfamily, the high diversity of species of this group in the Neotropical region, and the low number of Cassidinae s.l. species karyotyped so far, the aim of the present work was to establish the main mechanisms involved in the karyotype evolution of this subfamily through the study of seven species of the Brazilian fauna and overview of the cytogenetic data. The individuals were collected in southeast and south of Brazil. The chromosomal preparations obtained from embryo and testes of adult males were stained with Giemsa solution. The species Agroiconota inedita (2n=42=40+Xy(p)), Charidotella (s.str.) immaculata (2n=22=20+Xy(p)), Charidotella (s.str.) sexpunctata (2n=22=20+Xy(p)), and Stolas chalybaea (2n=24=22+Xy(p)) revealed diploid number higher than that established as basal for Polyphaga and biarmed chromosomes. The karyotype of Cteisella confusa, Deloyala cruciata, and Metriona elatior showed the chromosomal formulae 2n=18=16+Xy(p) considered modal for Cassidinae s.l. and biarmed chromosomes. The seven species exhibited easily identified sex chromosomes due to their size and/or morphology. The analysis of meiotic cells of all the species showed pachytenes with a positively heteropycnotic block probably corresponding to the sex chromosomes; diplotenes with a high number of bivalents with two

  6. A catalog of the types of Chrysomelidae sensu lato (Insecta, Coleoptera, Polyphaga deposited in the Museo Argentino de Ciencias Naturales, Buenos Aires Catálogo de los tipos de Chrysomelidae sensu lato (Insecta, Coleoptera, Polyphaga depositados en el Museo Argentino de Ciencias Naturales, Buenos Aires

    Directory of Open Access Journals (Sweden)

    Axel O. Bachmann

    2010-06-01

    Full Text Available The type specimens (all current categories of Chrysomelidae s.l. deposited in this Museum are listed; 125 names are recorded, 85 of them (68 percent are represented here by name-bearing types ('primary' types, five of them dubious. The family is taken in its broadest sense, including the Bruchinae, Hispinae (along with the former Cassidinae, and other groups sometimes considered as separate families. The specific and subspecific names were alphabetically filed, followed by the generic ones as they were spelled in the original publication, or the generic and specific names in the case of subspecies and varieties. Later combinations and/or current binomina are mentioned insofar as these are known to the authors. Two lists are added: 1. of specimens labelled as types of unavailable names, chiefly those not found in the literature, and supposedly not published, and 2. of specimens labelled as types, but not originally included as such, and published or not after the original description.Se catalogan los ejemplares típicos, de todas las categorías aceptadas, de Chrysomelidae s.l. conservados en este museo; se registran 125 nombres, 85 de ellos (68 por ciento representados aquí por tipos portadores de nombres (tipos 'primarios', cinco de ellos dudosos. La familia se toma en su concepto más amplio, incluyendo a las Bruchinae, las Hispinae (con las anteriores Cassidinae y otros grupos a veces considerados como familias separadas. Los nombres específicos y subespecíficos fueron ordenados alfabéticamente; a estos siguen los de los géneros, así como se publicaron originalmente, o de los géneros y especies en el caso de las subespecies y variedades. Se mencionan las combinaciones ulteriores y/o los binomios en uso, hasta donde son conocidos por los autores. Se agregan dos listas: 1. de los ejemplares rotulados como tipos, de nombres no disponibles, principalmente aquellos no hallados en la bibliografía, y supuestamente no publicados, y 2. de los

  7. ESPECIES DE DRYOPHTHORINAE (COLEOPTERA: CURCULIONIDAE ASOCIADAS A PLÁTANO Y BANANO (Musaspp. EN COLOMBIA Dryophthorinae Species (Coleoptera: Curculionidae Associted to Platain and Banana crops (Musaspp. in Colombia

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    PAULA A SEPÚLVEDA-CANO

    Full Text Available Se presenta una sinopsis de los escarabajos de la subfamilia Dryophthorinae (Coleoptera: Curculionidae asociados a cultivos de plátano y banano en Colombia. Adicionalmente se ofrecen claves ilustradas para las especies del país. Se registran seis especies asociadas a dichos cultivos: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.This synopsis is about beetle&#’;s subfamily Dryophthorinae (Coleoptera: Curculionidae associated to plantain and banana crops. Additionally keys illustrated for the species of the country are offered. Six species associated to these cultures are registered: Cosmopolites sordidus (Germar, 1824, Metamasius hemipterus (Linnaeus, 1758, Metamasius hebetatus (Gyllenhal, 1838, Metamasius submaculatus Champion, 1910, Rhyncophorus palmarum (Linnaeus, 1758 y Polytus mellerborgii (Boheman, 1838.

  8. Effect of arcelin protein on the biology of Zabrotes subfasciatus (Boheman 1833, in dry beans Efeito da proteína arcelina na biologia de Zabrotes subfasciatus (Boheman 1833, em feijoeiro

    Directory of Open Access Journals (Sweden)

    Flávia Rabelo Barbosa

    1999-10-01

    Full Text Available Arcelin is a seed protein found in wild beans (Phaseolus vulgaris which gives resistance to Mexican bean weevil, Zabrotes subfasciatus (Boheman 1833 (Coleoptera: Bruchidae. Studies were carried out with the objective of estimating the effect of four alleles of protein arcelin (Arc1, Arc2, Arc3 and Arc4 on the biology of Z. subfasciatus. The experiment was carried out in laboratory at Embrapa-Centro Nacional de Pesquisa de Arroz e Feijão, in Santo Antônio de Goiás, GO, Brazil, under non controlled conditions. The highest levels of antibiosis to Z. subfasciatus were observed in Arc1, with reduction in the number of eggs, number of emerged adults, adults longevity. In the line Arc2 only reduction in the number of emerged adults was observed. The lines Arc3 and Arc4 showed low efficiency on the reduction of progeny of Z. subfasciatus and effects in the longevity and egg-adult cycle were not detected. Insect sexual ratio was not altered by the presence of Arc1, Arc2, Arc3 and Arc4 in the seeds.A arcelina é uma proteína encontrada em feijões silvestres (Phaseolus vulgaris e que confere resistência ao caruncho-do-feijão, Zabrotes subfasciatus (Boheman 1833 (Coleoptera: Bruchidae. Estudos foram conduzidos com o objetivo de conhecer o efeito de quatro alelos da proteína arcelina (Arc1, Arc2, Arc3 e Arc4, na biologia de Z. subfasciatus. O experimento foi conduzido no laboratório da Embrapa-Centro Nacional de Pesquisa de Arroz e Feijão, no município de Santo Antônio de Goiás, GO, em condições não controladas. O mais alto nível de antibiose a Z. subfasciatus foi constatado na linhagem portadora do alelo Arc1, observando-se redução do número de ovos produzidos, redução do número de adultos emergidos, redução da longevidade de adultos. Na linhagem Arc2 constatou-se redução apenas no número de adultos emergidos. As linhagens Arc3 e Arc4 apresentaram baixa eficiência na redução da progênie de Z. subfasciatus, não observando

  9. Isolation and identification of Metarhizium anisopliae from natural infections of Coconut hispid beetle Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) , and preliminary studies on against this pest in Hainan island, China%海南椰心叶甲病原菌金龟子绿僵菌的分离、鉴定及其生防潜力

    Institute of Scientific and Technical Information of China (English)

    詹儒林; 覃伟权; 宋妍; 张世清; H H HO; 许天委; 黄俊生

    2007-01-01

    椰心叶甲[Brontispa longissima(Gestro)]是椰子的重要害虫,近年来,该虫在海南岛发生普遍,椰子受害严重.由于椰心叶甲受到自然界中某些致病微生物的侵袭,在受害的椰子树心叶上常可发现椰心叶甲僵虫,并发现大部分僵虫表面长出了霉菌,本研究的目的在于从椰心叶甲僵虫表面的霉菌中分离出绿僵菌,并对分离菌株进行鉴定和致病性测定.从僵虫表面刮下孢子或菌丝体,置于绿僵菌选择性培养基(DOA)上培养,挑出真菌菌落,经纯化后,进行生物学特性、菌落生长速率及产孢量的测定,并从PPDA、OMA、V8A和PDA中筛选菌落生长及产孢最适培养基,同时对所分离的菌株进行对椰心叶甲的致病性测定.结果表明,所有分离菌株均鉴定为金龟子绿僵菌[Metarhizium anisopliae(Metschnikoff)],PPDA是菌落生长及产孢的最适培养基,大多数菌株对椰心叶甲有较强的致病力.选取强毒菌株MA4在田间进行防治效果的初步测定,结果表明,该菌株能显著降低椰心叶甲成虫的虫口密度.这些金龟子绿僵菌菌株是首次从海南的椰心叶甲僵虫中分离到的昆虫病原真菌,该菌对海南的椰心叶甲具有很好的生防潜能.%Coconut hispid beetles, Brontispa longissima (Gestro) (Coleoptera:Chrysomelidae) have been prevalent all over Hainan Island in China, and millions of coconut palms were attacked by this pest. The objective of this work was to isolate and identify strains of entomopathogenic fungi from natural infections of B. longissima collected from coconut palms.Conidia or mycelia collected from the surface of beetle cadavers were cultured and the colonies were screened on dodine oatmeal agar medium (DOA). The colony morphology, mycelia growth rate and conidial production were further studied on various agar media: PPDA, OMA, V8A and PDA. In order to confirm whether these isolates were pathogenic to B.longissima, their virulence to the beetles were

  10. Chaves para a identificação dos principais Coleoptera (Insecta associados com produtos armazenados Keys for the identification of Coleoptera (Insecta associated with stored products

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Valle da Silva Pereira

    2001-03-01

    Full Text Available An illustrated key to identify nine families of Coleoptera commonly found in stored products is presented. Keys for the identification of Anobiidae [Lasioderma serricorne (Fabricius, 1792, Stegobium paniceum (Linnaeus, 1761], Bruchidae [Acanthoscelides obtectus (Say, 1831, Zabrotes subfasciatus (Boheman, 1833], Curculionidae [Sitophilus oryzae (Linnaeus, 1763, S. zeamais Motschulsky, 1885], Silvanidae [Ahasverus advena (Waltl, 1832, Cathartus quadricollis (Guérin, 1892, Oryzaephilus mercator (Fauvel, 1889, O. surinamensis (Linnaeus, 1758] and Tenebrionidae [Gnathocerus cornutus (Fabricius, 1798, Tribolium castaneum (Herbst, 1797, T. confusum du Val, 1868] are also provided. These keys cover the most frequent Coleoptera found in stored products, specially grains, and are to the adult stage only. Illustrations of external morphology and general characteristics are provided for each species reported.

  11. Especies mexicanas de Curculionidae (Insecta: Coleoptera asociadas con agaves (Asparagaceae: Agavoideae Mexican species of Curculionidae (Insecta: Coleoptera associated to agaves (Asparagaceae: Agavoideae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2012-12-01

    Full Text Available Se estudiaron las especies de picudos o gorgojos (Coleoptera: Curculionidae asociadas con agaves (Asparagaceae: Agavoideae en México. Se registraron 5 especies asociadas con especies de Agave, Furcraea, Hesperoyucca, Polianthes y Yucca; de éstas, 4 pertenecen a la subfamilia Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis y Cactophagus spinolae y 1 a la Baridinae (Peltophorus polymitus. Se presentan diagnosis, ilustraciones y una clave para la identificación de las 5 especies de curculiónidos asociados con agaves. Se establecen las siguientes sinonimias: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; y Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891 y Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.Weevils (Coleoptera: Curculionidae associated with agaves (Asparagaceae: Agavoideae from Mexico are analyzed. Five species were recorded associated with species of Agave, Furcraea, Hesperoyucca, Polianthes, and Yucca. Four of these species belong to the subfamily Dryophthorinae (Scyphophorus acupunctatus, S. yuccae, Rhinostomus frontalis, and Cactophagus spinolae and 1 belongs to the subfamily Baridinae (Peltophorus polymitus. Diagnoses, illustrations and a key are presented for identifying the 5 species of weevils found on agaves. The following synonymies are established: Sphenophorus validus LeConte, 1858 = Cactophagus spinolae (Gyllenhal, 1838; and Zygops polymitus seminiveus LeConte, 1884, Z. p. leopardinus Desbrochers, 1891, and Z. p. suffusus Casey, 1892 = Peltophorus polymitus Boheman, 1845.

  12. A New Microsporidian Parasite Record of Phyllotreta undulata (Chrysomelidae, Coleoptera)

    OpenAIRE

    YAMAN, Mustafa

    2005-01-01

    A new microsporidian parasite of Phyllotreta undulata was recorded in Turkey. Fresh spores are oval, 3.85 ± 0.69 µm (2.0-4.75) in length and 2.04 ± 0.23 µm (1.90-2.40) in width (n = 50). The spores are uninucleated. As there were insufficient data available on the complete life cycle of the parasite, it could not be identified at species level.

  13. Temporal distribution of Chaetocnema pulicaria (Coleoptera: Chrysomelidae) populations in Iowa.

    Science.gov (United States)

    Esker, P D; Obrycki, J; Nutter, F W

    2002-08-01

    In 1999 and 2000, yellow sticky cards and sweep net samples were used to document the occurrence of an overwintering adult generation of Chaetocnema pulicaria Melsheimer, corn flea beetle, followed by two distinct populations peaks during the growing season in Iowa Emergence of the overwintering adult generation started in mid-April and continued until early June in both years, with populations as high as 45 +/- 7.9 per 10 sweeps. Periods that ranged from 14 to 32 d were observed in 1999 and 2000 when C. pulicaria was not found following the overwintering generation. The first summer peak of C pulicaria was observed between the end of June into the middle of July, with the highest observed peak at 16.70 +/- 1.42 C. pulicaria per 10 sweeps in cornfields. The second summer peak of C pulicaria was observed between the middle into early September, with populations as high as 27.80 +/- 2.76 C. pulicaria per 10 sweeps. During the growing season, more C. pulicaria were caught on yellow sticky cards originating from soybean borders than from grass borders. There were significantly greater numbers of C. pulicaria on yellow sticky cards located in grass borders adjacent to cornfields at the end of the growing season, compared with yellow sticky cards located within cornfields, indicating the movement of C. pulicaria from the cornfield back into the grass borders at the end of the growing season. In 2000, from August to the end of the corn growing season, significantly more C. pulicaria were found in grass borders than in the cornfields. Based on this new quantitative information, planting time could be altered to avoid the emergence of the overwintering generation of C. pulicaria. In addition, knowledge concerning the seasonalities of the first and second population peaks of C pulicaria during the corn growing season could be used to recommend optimal timing for foliar-applied insecticide applications. This new knowledge concerning the seasonal dynamics of C pulicaria will help to improve management recommendations for Stewart's disease of corn, caused by the bacterium Pantoea stewartii, and that is vectored by C pulicaria. PMID:12216815

  14. Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae) *

    OpenAIRE

    Eduard Petitpierre

    2011-01-01

    Abstract Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xyp and XYp clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe T...

  15. New generic synonyms in the Oriental flea beetles (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    The following new synonyms are proposed for the genera of flea beetles from Oriental Region: Pseudocrypta Medvedev, 1996 and Sebaethiella Medvedev, 1993 = Acrocrypta Baly, 1862: 457; Bhutajana Scherer, 1979 = Aphthona Chevrolat, 1836; Burmaltica Scherer, 1969 = Aphthonaltica Heikertinger, 1924; Apht...

  16. Comportamento de Sternechus subsignatus (Boheman em dez espécies vegetais de verão para rotação de culturas ou cultura armadilha no plantio direto Sternechus subsignatus (Boheman behaviour in ten summer rotation specles and trapping crops for no-tillage system

    Directory of Open Access Journals (Sweden)

    Mauro Tadeu Braga da Silva

    1997-12-01

    Full Text Available O desenvolvimento de Sternechus subsignatus (Boheman (Coleoptera: Curculionidae foi avaliado em dez espécies vegetais sob condições naturais num solo manejado há três anos no sistema de plantio direto corn soja cultivada no verão. Foram observados o número de plantas atacadas, a oviposição, o núrnero e peso de larvas, o número de larvas hibernantes no solo e o número de adultos emergidos. O inseto se reproduziu e desenvolveu ern feijão (Phaseolus vulgaris L guandu anão (Cajanus cajan L, lab-lab (Dolichos lablab L. e soja (Glycine max L. e ao contrário, em crotalária júncea (Crotalaria júncea L. girassol (Helianthus annuus L., milheto (Pennisetum americanum Leek, milho (Zea mays L, mucuna preta (Stizolobium aterrimum Piper et Tracy e sorgo (Sorghum bicolor L. não completou o ciclo de vida. Os resultados indicaram que a cultura do feijão, guandu anão, lab-lab e soja aumentaram a população do inseto. Entretanto, ocorreu redução na população do inseto corn as culturas da crotalária júncea, girassol, milheto, milho, mucuna preta e sorgo. As quatro primeiras espécies são hospedeiras preferenciais, podendo ser usadas como culturas armadilhas e as demais, não são hospedeiras, pois propiciam a diminuição da população do inseto, sendo ideais para uso em sistemas derotação de culturas no verão, em áreas infestadas, para substituir o monocultivo da soja em plantio direto.The development of Sternechus subsignatus (Boheman (Coleoptera. Curculionidae was evaluated on ten crop species under natural conditions under no-tillage system for three years and infested with the insect Soybean was the Summer crop continously cultivated in the area. Number of attacked plants, oviposition, number and weight of larvae and number of adults emerged were the parameters evaluated. The insect reproduced and developed in bean (Phaseolus vulgaris L, grandull (Cajanus cajan L., dolichos (Dolichos lablab L. and soybean (Glycine max L. and

  17. Effect of gamma radiation on arcelin protein and its influence on Zabrotes Subfasciatus (Boheman)

    International Nuclear Information System (INIS)

    The research aimed to evaluate the effect of different doses of gamma radiation on the arcelin protein related to Zabrotes subfasciatus (Boheman) resistance. Seeds of Phaseolus vulgaris L. carrier of arcelin protein and a cv. without this protein, used as control, were irradiated with doses of 0, 0.25, 0.5, 1.0, and 2.0 kGy. After irradiation, the seeds were subjected to confinement test to evaluate different parameters viz. total egg, viable eggs, emerged insects, insect weights, oviposition period and sex ratio. The results showed no influence of radiation doses on the evaluated parameters, and the Arcelin-1 and 2 strains have maintained thus antibiosis grade. The Arcelin-3 and 4, also kept their less expressive behaviour of resistance by antibiosis and only prolonged the oviposition period of the female. (author)

  18. Taxonomia dos bruquíneos associados à Senna Neglecta (Vogel) H. S. Irwin e Barneby (Fabaceae: Caesalpinioideae), com revisão do grupo Abbreviatus de Sennis Bridwell (Coleoptera, Chrsomelidae, Bruchinae)

    OpenAIRE

    Viana, Jéssica Herzog Viana

    2011-01-01

    Resumo: Senna neglecta (Vogel) H. S. Irwin & Barneby ocorre nas regiões Nordeste, Sudeste e Sul do Brasil, em campos, vegetação secundária, borda e interior de mata. É uma planta estudada para uso em paisagismo, recuperação de áreas degradadas e produção de fármacos antimicrobiais. Um estudo taxonômico foi realizado com as espécies de Bruchinae (Chrysomelidae: Coleoptera) associadas à esta planta a partir de caracteres morfológicos do exoesqueleto e genitália masculina e feminina. Após levant...

  19. DANOS CAUSADOS POR LARVAS E ADULTOS DE DIABROTICA SPECIOSA (COLEOPTERA: CHRYSOMELIDAE EM MILHO DAMAGES CAUSED BY LARVAE AND ADULTS OF DIABROTICA SPECIOSA (COLEOPTERA: CHRYSOMELIDAE IN CORN

    Directory of Open Access Journals (Sweden)

    GILBERTO BATISTA CASTOR MARQUES

    1999-11-01

    Full Text Available A espécie Diabrotica speciosa (Germar, 1824 é, tradicionalmente, na fase adulta, uma praga polífaga, embora apresente certa preferência por folhas do feijoeiro e soja. Entretanto, nos últimos anos, a fase de larva deste crisomelídeo adquiriu o status de praga, à semelhança de outras espécies do mesmo gênero nos EUA, causando consideráveis danos ao sistema radicular do milho. O objetivo da pesquisa foi avaliar os danos causados por diferentes níveis populacionais de larvas de D. speciosa às raízes de milho, e, pelos adultos às folhas de milho, soja, feijoeiro e arroz. Desde as menores densidades populacionais de larvas, houve redução significativa no peso seco das raízes do milho, peso seco da parte aérea e na altura das plantas em relação à testemunha. Constatou-se que o nível de controle está aquém de 40 larvas por planta. Os adultos tiveram significativa preferência por folhas do feijoeiro e soja, sendo o milho e o arroz menos consumidos.Diabrotica speciosa (Germar, 1824 is traditionally a polyphagous pest during the adult phase although with some preference for bean and soybean leaves. In the past years, however, the larval phase of this chrysomelid achieved the pest status likewise other species of the genus in the USA causing severe damages to the root system of corn. The aim of this research was to evaluate the damages caused by different population levels of D. speciosa to corn roots and by adults to corn, soybean, bean and rice leaves. A significant decrease in the dry weight of corn roots, in the dry weight of the upper part, and in plant height in comparison with the control plant was observed even in small larvae population densities. The economic threshold level was less than 40 larvae per plant. The adults had significant preference for bean and soybean leaves while corn and rice were less consumed.

  20. Medicinal plant extracts on the control of Diabrotica speciosa (Coleoptera: Chrysomelidae Extratos de plantas medicinais no controle de Diabrotica speciosa (Coleoptera: Chrysomelidae

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    F.S. Barbosa

    2013-01-01

    Full Text Available The aim of this study was to evaluate the insecticidal effect of aqueous, alcoholic, and oil extracts from leaves of eight medicinal plants against Diabrotica speciosa prepared at five concentrations. The extracts that used commercial soybean oil as solvent showed the highest D. speciosa mortality due to the solvent itself, regardless of the used plants and their concentrations. Thus, commercial soybean oil was discarded as solvent since at these volumes it would cause serious phytotoxicity problems. After 24 hours of exposure of the pest to the extracts, the highest D. speciosa mortality values were observed for Copaifera langsdorfii and Chenopodium ambrosioides extracts, both in 5% alcohol, and Artemisia verlotorum, in 10% water. However, in the last mortality assessment (48 h, C. langsdorfii extract in 5% alcohol showed higher mortality of this pest, followed by C. ambrosioides extract in 5% alcohol, compared to the remaining plants.O objetivo deste trabalho foi avaliar o efeito inseticida de extratos aquosos, alcoólicos e oleosos de folhas de oito plantas medicinais contra Diabrotica speciosa preparadas em cinco concentrações. Os extratos que utilizaram óleo de soja comercial como solvente apresentaram as maiores mortalidades de D. speciosa em função do próprio óleo, independentemente das plantas utilizadas em suas concentrações. Sendo assim, o óleo de soja comercial foi descartado como solvente, pois nestes volumes acarretaria sérios problemas de fitotoxidade. Após 24 horas de exposição da praga aos extratos, os maiores valores de mortalidade de D. speciosa foram observados nos extratos de Copaifera Langsdorfii e de Chenopodium ambrosioides, ambos em álcool 5%, e de Artemisia verlotorum, em água 10%. Entretanto, na última avaliação de mortalidade (48 h, o extrato de C. langsdorfii em álcool a 5% apresentou maior mortalidade dessa praga, seguida pelo extrato alcoólico a 5% de C. ambrosioides comparada às demais plantas.

  1. Desenvolvimento de Diabrotica speciosa (Germar (Coleoptera: Chrysomelidae em diferentes hospedeiros Development of Diabrotica speciosa (Germar (Coleoptera: Chrysomelidae in different host plants

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    Crébio José Ávila

    2002-10-01

    Full Text Available Este trabalho teve como objetivo determinar a influência do hospedeiro, sobre o desenvolvimento de larvas, adultos e a reprodução de D. speciosa. Larvas recém-eclodidas foram criadas em "seedlings" de feijoeiro, soja, milho e batata enraizada, utilizando-se, como substrato, vermiculita esterilizada e umedecida. Determinou-se a duração e viabilidade do período larva-adulto e o peso de insetos (machos e fêmeas recém-emergidos. Em outro experimento, foi avaliada a capacidade de postura de D. speciosa. Para isso, ofereceram-se aos adultos folhas de feijoeiro, soja, milho e batata. A duração e a viabilidade do período larva-adulto, bem como o peso dos insetos (machos e fêmeas recém-emergidos, foram significativamente influenciados pela espécie hospedeira utilizada como alimento na fase larval. O maior período de desenvolvimento das fases imaturas (larva + pupa foi verificado em tubérculo de batata (36,5 dias e o menor, em "seedlings" de milho (25,1 dias. Os maiores valores de viabilidade foram observadas em batata (84,1% e milho (75,9%, enquanto que em soja (30,1% e feijoeiro (9,4% foram bastante reduzidos, mostrando serem estes dois hospedeiros inadequados para o desenvolvimento imaturo (larva + pupal de D. speciosa. Os insetos alimentados com folhas de batata e feijoeiro apresentaram capacidades de postura significativamente superiores àqueles mantidos em folhas de soja ou milho. Verificou-se que "seedlings" de milho e radicelas de batata são adequados, como alimento, para larvas de D. speciosa, enquanto folhas de batata e feijão são os mais adequados para os adultos.The aim of this work was to determine the influence of the host plant, as food, on the larval and adult phases of D. speciosa. Larvae of this insect were reared on seedlings of bean, corn, soybean, as well as on potato tubers kept in wet vermiculite. The following biological parameters were evaluated: duration and viability of the larva-adult period and the weight of newly emerged insects (males and females. The fecundity of D. speciosa was also evaluated offering to the adults leaves of bean, soybean, corn and potato. The host plant offered as food on the larval phase influenced significantly the weight of the insects (males and females as well as the duration and viability of the larva-adult period. The development time of the immature phases (larva + pupa on potato (36.5 days was longer than that on corn (25.1 days. The viability values (larva + pupa were higher on potato (84.1% and corn (75.9% while on soybean and bean the viability was low (30.1% and 9.4%, respectively, characterizing these plants as inadequate hosts for the larval development of the insect. Adults of D. speciosa fed on potato and bean leaves presented fecundity significantly superior to that on soybean or corn leaves. Therefore, corn seedlings and potato tubers are appropriate to larval development of D. speciosa, while leaves of potato and bean are suitable for adults.

  2. Trap attributes influencing capture of Diabrotica speciosa (Coleoptera: Chrysomelidae on common bean fields Atributos da armadilha influenciam captura de Diabrotica speciosa (Coleoptera: Chrysomelidae em feijoeiro

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    Maurício Ursi Ventura

    2005-08-01

    Full Text Available Refinements in trap characteristics may improve ability to monitor and mass-trap beetles. Field assays were conducted in common bean fields to assess responses of Diabrotica speciosa (Germar to some trap characteristics. Golden yellow plastic cups (750 mL traps caught more D. speciosa females and males than did clear traps. Carrot slices in Petri dishes baited with Lagenaria vulagaris L. powder (cucurbitacin source - 0.28% caught more beetles than did dishes with carrot alone. Dispensers for the floral volatile attractant 1,4-dimethoxybenze were also compared. Rubber septa dispenser attracted more beetles than did control (dental wicks saturated with acetone. Captures on dental wick, starch matrix and feminine pad dispensers were intermediate and did not differ from those on rubber septa and unbaited controls. Perforated bottle traps (2000 mL, when baited with the floral attractant, caught more beetles than did window bottle traps (both traps contained L. vulgaris powder in most assessments done from two to ten days after trap placement in the field. Traps with the insecticide carbaryl captured more beetles than did traps without it, 2-4 and 8-10 days after trap placement in the field, but not in the remaining periods (0-2, 4-6 and 6-8 days. Traps baited with 1,4-dimethoxybenzene captured more beetles than did the unbaited ones in all assessments (each other day from two to ten days after trap placement in the field. Finally, similar amounts of beetles were captured using plastic bottle traps (2000 mL: perforated, window (both with cucurbitacin and sticky (without cucurbitacin traps, when were baited with the floral attractant.Refinamentos em caraterísticas de armadilhas podem incrementar sua habilidade para monitorar e capturar em massa os insetos. Experimentos foram conduzidos em lavoura de feijoeiro para verificar as respostas de Diabrotica speciosa (Germar a algumas características de armadilhas. Armadilhas de copos plástico (750 mL amarelo ouro capturaram mais fêmeas e machos de D. speciosa do que transparentes. Placas de Petri com pedaços de cenoura, com pó seco de purungo, Lagenaria vulgaris L. (fonte de cucurbitacina - 0,28% capturaram mais insetos do que placas com somente cenoura. Liberadores para o atraente floral 1,4-dimetoxibenzeno foram também comparados. Liberador de septo de borracha atraiu mais insetos do que o controle (flocos dentais saturados com acetona. Capturas nos tratamentos com liberadores de floco dental, matriz borato e absorvente higiênico foram intermediárias mas não diferiram da testemunha e septo. Modelo de armadilha de garrafa (2000 mL furada capturou mais insetos do que armadilha vazada ("janelas" (ambas contendo pó seco de purungo na maioria das avaliações, dos dois até os dez dias após a instalação das armadilhas no campo. Armadilhas com inseticida carbaryl capturaram mais insetos do que armadilhas sem, nos intervalos de 2-4 e 9-10 dias após a instalação das armadilhas no campo. Nos períodos restantes (0-2, 4-6- e 6-8 dias, diferenças não foram detectadas. Armadilhas com 1,4-dimetoxibenzeno capturaram mais insetos do que armadilhas sem o volátil (avaliações a cada dois dias dos dois aos dez dias após a instalação no campo. Finalmente, quantidades similares de insetos foram capturadas usando armadilhas de garrafa pet (2000 mL: vazadas ("janelas", furadas (ambas com cucurbitacina e adesivas quando estas foram iscadas com o atraente floral.

  3. EXTRATO DE NEEM E CRAVO DA ÍNDIA NO CONTROLE DE ZABROTES SUBFASCIATUS (BOHEMAN) (COLEOPTERA: BRUCHIDAE) EM SEMENTES DE FEIJÃO ARMAZENADO

    OpenAIRE

    Beatriz Aguiar Jordão Paranhos; Ceci Castilho Custódio; Nelson Barbosa Machado Neto; Aleandro Santana Rodrigues

    2005-01-01

    The bean weevil (Zabrotes subfasciatus) is considered the principal storage pest of beans. The objective of this work was the study of alternatives to control the Zabrotes subfasciatus in beans, using neem seed (Azadirachta indica) extract and Indian spice (Caryophyllus aromaticus L.). Four treatments were used: control; 1% aqueous neem solution (0,5ml neem oil .Kg-1 of bean seed), 25g of spice in one kg of bean seed and Gastoxin (0,011g aluminum phosphet.Kg-1 of bean seed). The experiment w...

  4. Alternative food sources and over wintering feeding behavior of the boll weevil, Anthonomus grandis boheman (coleoptera: curculionidae) under the tropical conditions of central Brazil

    International Nuclear Information System (INIS)

    The boll weevil causes serious damage to the cotton crop in South America. Several studies have been published on this pest, but its phenology and behavior under the tropical conditions prevailing in Brazil are not well-known. In this study the feeding behavior and main food sources of adult boll weevils throughout the year in Central Brazil was investigated. The digestive tract contents of insects captured in pheromone traps in two cotton fields and two areas of native vegetation (gallery forest and cerrado sensu stricto) were analyzed. The insect was captured all through the year only in the cerrado. It fed on pollen of 19 different plant families, on Pteridophyta and fungi spores and algae cysts. Simpson Index test showed that the cerrado provided greater diversity of pollen sources. In the beginning of the cotton cycle, the plant families used for pollen feeding were varied: in cotton area 1, the weevil fed on Poaceae (50%), Malvaceae and Smilacaceae (25% each); in cotton area 2 the pollen sources were Malvaceae (50%), Asteraceae (25%) and Fabaceae and Clusiaceae (25% each); in the cerrado they were Chenopodiaceae (67%) and Scheuchzeriaceae (33%). No weevils were collected in the gallery forest in this period. After cotton was harvested, the family Smilacaceae was predominant among the food plants exploited in all the study areas. These results help to explain the survivorship of adult boll weevil during cotton fallow season in Central Brazil and they are discussed in the context of behavioral adaptations to the prevailing tropical environmental conditions. (author)

  5. Effect of garlic extraction on injury by cowpea, Curculio Chalcodermes aenus Boheman (Coleoptera: Cucurlionidae), and other pests, to cowpea, Vigna unguiculata L. Walp

    Science.gov (United States)

    Garlic-based oils and extract formulations have been used as insecticides against various insects on numerous crops, but there are contradictions among findings on the insecticidal or repellent properties of garlic-based products. In a field plot test, the effects of garlic extract on control of th...

  6. Alternative food sources and over wintering feeding behavior of the boll weevil, Anthonomus grandis boheman (coleoptera: curculionidae) under the tropical conditions of central Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Paulina de A.; Sujii, Edison R.; Pires, Carmen S.S.; Fontes, Eliana M.G. [EMBRAPA Recursos Geneticos e Biotecnologia (CENARGEN), Brasilia, DF (Brazil)], e-mail: paulina723@hotmail.com, e-mail: sujii@cenargen.embrapa.br, e-mail: cpires@cenargen.embrapa.br, e-mail: eliana@cenargen.embrapa.br; Diniz, Ivone R. [Universidade de Brasilia (UnB), DF (Brazil). Dept. de Zoologia], e-mail: irdiniz@unb.br; Medeiros, Maria A. de; Branco, Marina C. [EMBRAPA Hortalicas, Brasilia, DF (Brazil)], e-mail: medeiros@cnph.embrapa.br, e-mail: marina@cnph.embrapa.br; Salgado-Labouriau, Maria L. [Universidade de Brasilia (UnB), DF (Brazil). Dept. de Geologia], e-mail: mlea@unb.br

    2010-01-15

    The boll weevil causes serious damage to the cotton crop in South America. Several studies have been published on this pest, but its phenology and behavior under the tropical conditions prevailing in Brazil are not well-known. In this study the feeding behavior and main food sources of adult boll weevils throughout the year in Central Brazil was investigated. The digestive tract contents of insects captured in pheromone traps in two cotton fields and two areas of native vegetation (gallery forest and cerrado sensu stricto) were analyzed. The insect was captured all through the year only in the cerrado. It fed on pollen of 19 different plant families, on Pteridophyta and fungi spores and algae cysts. Simpson Index test showed that the cerrado provided greater diversity of pollen sources. In the beginning of the cotton cycle, the plant families used for pollen feeding were varied: in cotton area 1, the weevil fed on Poaceae (50%), Malvaceae and Smilacaceae (25% each); in cotton area 2 the pollen sources were Malvaceae (50%), Asteraceae (25%) and Fabaceae and Clusiaceae (25% each); in the cerrado they were Chenopodiaceae (67%) and Scheuchzeriaceae (33%). No weevils were collected in the gallery forest in this period. After cotton was harvested, the family Smilacaceae was predominant among the food plants exploited in all the study areas. These results help to explain the survivorship of adult boll weevil during cotton fallow season in Central Brazil and they are discussed in the context of behavioral adaptations to the prevailing tropical environmental conditions. (author)

  7. Striped Cucumber Beetle (Coleoptera: Chrysomelidae) Aggregation in Response to Cultivar and Flowering.

    Science.gov (United States)

    Gardner, Jeffrey; Hoffmann, Michael P; Mazourek, Michael

    2015-04-01

    The striped cucumber beetle [Acalymma vittatum (F.)] is a specialist pest of cucurbits throughout its range in the United States and Canada. Improved integrated pest management options are needed across the pest's range, especially on organic farms where there are few effective controls. Trap cropping in cucurbits is an option, but there are significant challenges to the technique. Because cucurbit flowers are highly attractive to the beetles, four field experiments tested whether cultivar and phenology interact to preferentially aggregate beetles. The first experiment tested the hypothesis that cucurbit flowers were more attractive to striped cucumber beetles than was foliage. The second experiment tested whether there were differences in beetle aggregation between two relatively attractive cultivars. The third and fourth experiments were factorial designs with two plant cultivars and two levels of flowering to specifically test for an interaction of cultivar and flowering. Results indicated that flowers were more attractive than foliage, beetle aggregation was affected by plant cultivar, and that there was an interaction of cultivar with flowering. We conclude that a single cultivar may be sufficient to serve as a generic trap crop to protect a wide variety of cucurbits. PMID:26313184

  8. Management of Yellowmargined Leaf Beetle Microtheca ochroloma (Coleoptera: Chrysomelidae) Using Turnip as a Trap Crop.

    Science.gov (United States)

    Balusu, Rammohan; Rhodes, Elena; Liburd, Oscar; Fadamiro, Henry

    2015-12-01

    The yellowmargined leaf beetle, Microtheca ochroloma Stål, is a major pest of cruciferous vegetable crops in organic production systems. Very few organically acceptable management options are currently available for this pest. Field studies were conducted at a research station in Alabama and at a commercial organic vegetable farm in Florida to investigate the effectiveness of turnip, Brassica rapa rapa, as a trap crop for M. ochroloma. In the research station trial with cabbage planted as the cash crop, perimeter planting of turnip as a trap crop effectively reduced beetle numbers and crop damage below levels recorded in the control. During the first season of our on-farm trial, with napa cabbage and mustard as the cash crops, using turnip as a trap crop effectively reduced both beetle numbers and cash crop damage below levels found in the control plots, but economic damage was still high. In the second season, beetle populations were too low for significant differences in damage levels to occur between the trap crop and control plots. Together, these results suggest that turnip planted as a trap crop can be an effective control tactic for cruciferous crops, like cabbage, that are much less attractive to M. ochroloma than turnip. In crops, like mustard and napa cabbage, that are equally or only slightly less attractive than turnip, planting turnip as a trap crop would have to be used in combination with other tactics to manage M. ochroloma. PMID:26470380

  9. Methyl anthranilate as a repellent for western corn rootworm larvae (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Methyl anthranilate was identified as the active compound in extracts of maize roots that were shown to be repellent to neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae. A bioassay-driven approach was used to isolate the active material from diethyl ether extracts of r...

  10. A chromosomal analysis of four species of Chilean Chrysomelinae (Coleoptera, Chrysomelidae

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    Eduard Petitpierre

    2012-10-01

    Full Text Available Four species of Chilean leaf beetles in the subfamily Chrysomelinae have been cytogenetically analyzed, Blaptea elguetai Petitpierre, 2011, Henicotherus porteri Bréthes, 1929 and Jolivetia obscura (Philippi, 1864 show 2n = 28 chromosomes and a 13 + Xyp male meioformula, and Pataya nitida (Philippi, 1864 has the highest number of 2n = 38 chromosomes. The karyotype of H. porteri is made of mostly small meta/submetacentric chromosomes, and that of Jolivetia obscura displays striking procentric blocks of heterochromatin at pachytene autosomic bivalents using conventional staining. These findings are discussed in relation to previous cytogenetic data and current taxonomy of the subfamily.

  11. Trap height and orientation of yellow sticky traps affect capture of Chaetocnema pulicaria (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Esker, P D; Obrycki, J; Nutter, F W

    2004-02-01

    Field studies were conducted in Iowa during 2001 and 2002 to determine the optimal sampling height and orientation for using yellow sticky cards to monitor populations of Chaetocnema pulicaria Melsheimer, the vector of the bacterial pathogen Pantoea stewartii subsp, stewartii, the causal organism of Stewart's disease of corn, Zea mays L.. Sticky cards were placed at five different heights (0.15, 0.30, 0.45, 0.60, and 0.90 m) and three orientations (horizontal, vertical, and 30 degree angle) at three locations (Ames, Crawfordsville, and Sutherland) in 2001 and two locations (Crawfordsville and Johnston) in 2002. No statistical differences were observed among the placement combinations for individual sampling periods or for the total number of C. pulicaria captured in 2001. In 2002, the 0.30 m and vertical cards captured significantly (1.1-35 times) more C. pulicaria than any other placement combination during sampling throughout August at both Crawfordsville and Johnston. Also, the cumulative number of C. pulicaria captured by the 0.30 m and vertical cards was significantly higher than all other placement combinations. This information is important in the development of sampling protocols to aid growers in making management decisions. These management decisions include where and when to apply foliar insecticides during the corn growing season to control C. pulicaria populations, thereby reducing the risk for Stewart's disease of corn. PMID:14998138

  12. Using trap crops for control of Acalymma vittatum (Coleoptera: Chrysomelidae) reduces insecticide use in butternut squash.

    Science.gov (United States)

    Cavanagh, A; Hazzard, R; Adler, L S; Boucher, J

    2009-06-01

    Striped cucumber beetle, Acalymma vittatum F., is the primary insect pest of cucurbit crops in the northeastern United States. Adult beetles colonize squash crops from field borders, causing feeding damage at the seedling stage and transmitting bacterial wilt Erwinia tracheiphila Hauben et al. 1999. Conventional control methods rely on insecticide applications to the entire field, but surrounding main crops with a more attractive perimeter could reduce reliance on insecticides. A. cittatum shows a marked preference for Blue Hubbard squash (Cucurbita maxima Duchesne) over butternut squash (C. moschata Poir). Given this preference, Blue Hubbard squash has the potential to be an effective perimeter trap crop. We evaluated this system in commercial butternut fields in 2003 and 2004, comparing fields using perimeter trap cropping with Blue Hubbard to conventionally managed fields. In 2003, we used a foliar insecticide to control beetles in the trap crop borders, and in 2004, we compared systemic and foliar insecticide treatments for the trap crop borders. We found that using a trap crop system reduced or eliminated the need to spray the main crop area, reducing insecticide use by up to 94% compared with conventional control methods, with no increase in herbivory or beetle numbers. We surveyed the growers who participated in these experiments and found a high level of satisfaction with the effectiveness and simplicity of the system. These results suggest that this method of pest control is both effective and simple enough in its implementation to have high potential for adoption among growers. PMID:19610425

  13. Methyl Anthranilate as a Repellent for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Bernklau, E J; Hibbard, B E; Norton, A P; Bjostad, L B

    2016-08-01

    Methyl anthranilate was identified as the active compound in extracts of maize (Zea mays L.) roots that were shown to be repellent to neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae. A bioassay-driven approach was used to isolate the active material from diethyl ether extracts of roots from germinating maize seeds. Separation of the extract on a Florisil column yielded an active fraction of 90:10 hexane:diethyl ether. Analysis with gas chromatography-mass spectrometry identified two compounds in the active fraction: indole (2,3-benzopyrrole) and methyl anthranilate (methyl 2-aminobenzoate). When tested in behavioral bioassays, methyl anthranilate elicited a significant (P repellent response at doses of 1, 10, and 100 µg. In subsequent single-choice bioassays, 1, 10, and 100 µg of methyl anthranilate prevented larvae from approaching 10 mmol/mol concentrations of carbon dioxide, which is normally highly attractive to the larvae. Indole, the other compound identified from the active fraction, did not elicit a behavioral response by the larvae. Methyl anthranilate has potential for development as a management tool for western corn rootworm larvae and may be best suited for use in a push-pull control strategy. PMID:27122493

  14. Amphimela raydahensis sp. nov. from the Kingdom of Saudi Arabia (Coleoptera: Chrysomelidae: Galerucinae: Alticini).

    Science.gov (United States)

    Torkey, Ashraf M El; Dhafer, Hathal M Al

    2015-01-01

    Amphimela raydahensis sp. nov. is described from Garf Raydah Nature Reserve (Abha, Asir Province), southwestern Kingdom of Saudi Arabia. This species was collected using black light traps at different elevations dominated by cacti and wild olive trees. This is the first confirmed species of Amphimela known from the Arabian Peninsula. PMID:26624320

  15. Egg-hatching synchrony and larval cannibalism in the dock leaf beetle Gastrophysa viridula (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Kutcherov, Dmitry

    2015-12-01

    Females of leaf beetles and many other herbivorous insects lay eggs in coherent batches. Hatchlings emerge more or less simultaneously and often prey on their late-hatching clutchmates. It is not certain, however, whether this synchrony of hatching is a mere by-product of cannibalism or whether an additional synchronizing factor exists. The following simple experiment was aimed at determining the causal relationship between cannibalism and simultaneous larval emergence. Egg clutches of the dock leaf beetle Gastrophysa viridula were split into two halves. These halves were either kept as coherent groups in two separate dishes or, alternatively, only one half remained whole, whereas the other one was divided into single eggs, each of which was incubated in a separate dish. Halving of a clutch into coherent groups only slightly disrupted the synchrony of emergence. The consequence of individual isolation was more dramatic. Half-clutches consisting of disconnected solitary eggs required almost twice as much time for complete emergence of all larvae, which was significantly more than cannibalism as a sole synchronizing factor might explain. Moreover, survival rates were the same in coherent half-clutches (in the presence of cannibalism) and among isolated individuals. This group effect and the small contribution of cannibalism suggest the existence of an additional synchronizing factor. Possible mechanisms underpinning this phenomenon are discussed. PMID:26482400

  16. The Genus Coelocrania and the Resurrection of the Genus Pseudosastra (Coleoptera: Chrysomelidae: Galerucinae)

    OpenAIRE

    Mohamed S. Mohamedsaid

    1993-01-01

    The genus Coelocrania Jacoby is reviewed, with the resurrection of the genus Pseudosastra Jacoby. Four new species are herein described from Malaysia: Pseudosastra depressa, P. ghazallyi , P. indah, and P. latiffi. Keys to the species of Coelocrania and Pseudosastra are provided.

  17. Effectiveness of Corn Rootworm (Coleoptera: Chrysomelidae) Areawide Pest Management in South Dakota

    Science.gov (United States)

    Diabrotica barberi Smith & Lawrence and D. v. virgifera virgifera LeConte are serious pests of maize (Zea mays L.). The U. S. Department of Agriculture, Agricultural Research Service implemented a five year (1997-2001) areawide pest management program in five geographic locations, including one in S...

  18. A new genus and species of Eumolpinae (Coleoptera: Chrysomelidae) from the western dry forest of Ecuador

    OpenAIRE

    R. Wills Flowers

    2009-01-01

    Australotymnes jipijapa new genus, new species (type locality: Ecuador) is described. Adults were collected from vegetation during the rainy season in disturbed and converted dry forest in western Ecuador.

  19. Effect of Larval Density on Development of the Coconut Hispine Beetle, Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae)

    OpenAIRE

    Mika Murata; Dang Thi Dung; Shun-ichiro Takano; Ryoko Tabata Ichiki; Satoshi Nakamura

    2012-01-01

    The coconut hispine beetle, Brontispa longissima shows, aggregation in the field. To elucidate the effect of aggregation on larval developmental aspects, we examined the effects of larval density on various aspects of larval development and on survival rates. Recently we found that B. longissima was divided into two monophyletic clades by genetic analysis. Therefore, we also compared the results between two populations, from Ishigaki, Japan (ISH) and Papua New Guinea (PNG), which were represe...

  20. Population Dynamics of Bean Leaf Beetle, Cerotoma trifurcata (Coleoptera: Chrysomelidae on Edamame Soybean Plants In Nebraska

    Directory of Open Access Journals (Sweden)

    Bamphitlhi Tiroesele

    2013-02-01

    Full Text Available Edamame soybeans are a speciality food item for fresh and processed markets and they are harvested at a physiologically immature (R6 stage. Bean leaf beetle, Cerotoma trifurcata, is a sporadic pest of soybean in Nebraska, however, its pest status and abundance has increased in the recent years due to an increase in soybean acreage. This was a field experiment aimed at determining the population growth rate of bean leaf beetle on two edamame soybean cultivars, ‘Butterbeans’ and ‘Envy,’ at two planting dates during 2004 and 2005 in Nebraska. The population growth of beetles was significantly higher on 'Butterbeans' than on 'Envy' for both the first and second planting periods in both 2004 and 2005 seasons. The beetle infestation differences were noticed on plants at the late reproductive growth stages, R5 and R6. Additionally, the beetle infestation on 'Butterbeans' growth stages in 2004 and 2005 was significantly different for the first and second planting dates. On average, the beetles were higher on plants at the late reproductive stages than the other stages for first and second planting periods. Similarly, ‘Envy’ growth stages showed significant difference in beetle infestation during the first and second planting dates. Significantly high beetle infestations were observed at the vegetative growth stages. The study revealed that population growth of bean leaf beetles on edamame soybeans is affected by the planting date, season and cultivar choice.

  1. Banded cucumber beetle (Coleoptera: Chrysomelidae) resistance in romaine lettuce: understanding latex chemistry

    Science.gov (United States)

    Many plants subjected to herbivore damage exude latex, a rich source of biochemicals, which play important roles in host plant resistance. Our previous studies showed that fresh latex from Valmaine, a resistant cultivar of romaine lettuce Lactuca sativa L., applied to artificial diet is highly deter...

  2. Ecotoxicological hazards of herbicides on biological attributes of Zygogramma bicolorata Pallister (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Hasan, Fazil; Ansari, M Shafiq

    2016-07-01

    Ecotoxic effects of commonly used herbicides i.e. glyphosate, atrazine, metribuzin, alachlor and 2, 4-D were evaluated on the biological and demographic parameters of Zygogramma bicolorata Pallister on parthenium in laboratory. The herbicides used in the bioassay were within a minimum range of their recommended field dose. In direct toxicity experiment, 2,4-D and alachlor caused the highest mortality of 3rd instars and prolonged the development time. Fecundity and eggs viability were also significantly reduced in all treatments. Indirect toxicity (carryover effect) was evaluated through life table analysis of F1 progenies developed from surviving 3rd instars treated for direct toxicity experiment. Incubation period and overall development time was considerably prolonged in all treatments especially in 2, 4-D treated group. Daily fecundity and population growth parameters were significantly lowest in 2, 4-D treated groups compared to other tested herbicides. A significantly greater number of females were produced in glyphosate treatment than other treatments. Based on the present study, none of the tested herbicide can be classified as safe to Zygogramma bicolorata, while glyphosate was found to be least toxic. Therefore, it can be concluded that application of field recommended dose of glyphosate might be used in integration with Z. bicolorata for effective management of parthenium, however this needs to be conducted under natural field conditions. PMID:27077536

  3. Effects of Potato Cultivars on Some Physiological Processes of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Mardani-Talaee, Mozhgan; Zibaee, Arash; Nouri-Ganbalani, Gadir; Rahimi, Vahid; Tajmiri, Pejman

    2015-10-01

    Colorado potato beetle, Leptinotarsa decemlineata (Say), is an important pest of potato throughout the world. Here, the effects of six potato cultivars including 'Arinda,' 'Sprit,' 'Markiez,' 'Lotta,' 'Santae,' and 'Agria' were studied on nutritional indices, digestive enzymes, and some components involved in intermediary metabolism of L. decemlineata. Nutritional indices of the larvae and the adults were significantly different followed by feeding on various potato cultivars. The individuals fed on Agria showed the highest activity of digestive proteases although cathepsin B demonstrated same Activity on Santae and Lotta. The highest activity of α-amylase was found in the larvae fed on Arinda, but the adults demonstrated the highest amylolytic activities on Santae and Agria. Both larvae and adults of L. decemlineata fed on Santae revealed the highest α- and β-glucosidase activities. No significant differences were found in lipase activity of larvae, but the highest lipase activity was found in the adults fed on Santae. The highest activities of transaminases were found in the larvae and adults fed on the Agria except for γ-glutamyl transferase. In case of aldolase, the highest activities were observed in the larvae and adults fed on Santae and Sprit. The highest activities of lactate dehydrogenase were obtained in the larvae and adults fed on Santae. The highest amount of low-density lipophorin was measured in both individuals fed on Santae. There were no significant differences in high-density lipophorin amount of adults, but the highest value was found in the larvae fed on Agria. The lowest amounts of protein and triglyceride were observed in both individuals fed on Santae and Agria, respectively. These results revealed Santae is the most suitable cultivar for L. decemlineata based on digestion and intermediary metabolism findings, but Lotta is an unsuitable cultivar and could be considered for integrated pest management. PMID:26453726

  4. Host plant selection of Chrysolina clathrata(Coleoptera:Chrysomelidae)from Mpumalanga,South Africa

    Institute of Scientific and Technical Information of China (English)

    Robert S.Boyd; Micheal A.Davis; Michael A.Wall; Kevin Balkwill

    2009-01-01

    Hyperaccumulated elements such as Ni may defend plants against some natural enemies whereas other enemies may circumvent this delense.The Ni hyperaccumulator Berkheya coddif Roessler(Asteraceae)is a host plant species for Chrysolina clathrata (Clark),which Suffers no apparent harm by consuming its leaf tissue.Beetle specimens collected from B.coddii had a whole body Ni concentration of 260 μg/g dry weight.despite consuming leaf material containing 15 100μg Ni/g.Two experiments were conducted with adults of this beetle species:a no-choice experiment and a choice experiment.In the no-choice experiment we offered beetles foliage of one of four species of Berkheya:B.coddii.B.rehmannii Thell.Var.rogersiana Thell.,B.echinacea(Harv.)O.Hoffm.ex Burtt Davey,and B.insignis(Harv.)Thell.The two former species are Ni hyperaccumulators(defined as having leaf Ni concentration>1 000Pμ/g)whereas the latter have low Ni levels(<200μg/g)in their leaves.Masses of beetles were monitored for 6 days.Choice experiments used growing stem tips from the same Berkheya species.placed into Petri dishes with five Chrysolina beetles in each.and the amount of feeding damage caused on each of the four species was recorded.Beetles in the no-choice experiment gained mass when offered B.coddii,maintained mass on lcaves of the other Ni hyperaccumulator (B.rehmannif vaF.rogersiana),and lost mass when offered non-hyperaccumulator leaves.In the choice test.beetles strongly preferred B.coddii to other Berkheya species.We conclude that C clathrata may be host-specific on B.coddii.

  5. A new genus of moss inhabiting flea beetles (Coleoptera: Chrysomelidae) from Nicaragua

    Science.gov (United States)

    Nicaltica new genus and new species (N. selvanegra), from Nicaragua are described and illustrated. Nicaltica is compared to Kiskeya Konstantinov and Chamorro, Monotalla Bechyne, and Normaltica Konstantinov....

  6. Early Detection and Mitigation of Resistance to Bt Maize by Western Corn Rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Andow, David A; Pueppke, Steven G; Schaafsma, Arthur W; Gassmann, Aaron J; Sappington, Thomas W; Meinke, Lance J; Mitchell, Paul D; Hurley, Terrance M; Hellmich, Richard L; Porter, R Pat

    2016-02-01

    Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges. Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, and more general concerns around Bt crops that produce less than a high-dose of Bt toxin, have caused uncertainty around current IRM protocols. More than 15 years of experience with IRM has shown that high-dose and refuge-based IRM is not applicable to Bt crops that produce less than a high-dose. Adaptive IRM approaches and pro-active, integrated IRM-pest management strategies are needed and should be in place before release of new technologies that produce less than a high-dose. We suggest changes in IRM strategies to preserve the utility of corn rootworm Bt maize by 1) targeting local resistance management earlier in the sequence of responses to resistance and 2) developing area-wide criteria to address widespread economic losses. We also favor consideration of policies and programs to counteract economic forces that are contributing to rapid resistance evolution. PMID:26362989

  7. Unikaryon phyllotretae sp. n. (Protista, Microspora), a new microsporidian pathogen of Phyllotreta undulata (Coleoptera; Chrysomelidae).

    Science.gov (United States)

    Yaman, Mustafa; Radek, Renate; Weiser, Jaroslav; Toguebaye, Bhen Sikina

    2010-01-01

    The microsporidium Unikaryon phyllotretae sp. n., a new pathogen of Phyllotreta undulata, is described based on light microscopic and ultrastructural characteristics. Microscopic examination of parasitized individuals revealed two types of spores. The majority of the spores were of the first type, which are oval and measured 2.74+/-0.17 x 1.93+/-0.17 microm when fresh. Fresh spores of the second type (very rare) are elongated and measured 4.39+/-0.18 x 1.61+/-0.20 microm. All life stages have single nuclei. Sporogony ends with uninucleate single sporoblasts and spores. The spores were only observed in Malpighian tubules. The isofilar polar filament of the parasite has six to eight coils, and a well-developed polaroplast was of the lamellated type, with closely packed anterior lamellae and loosely packed posterior lamellae. PMID:19767185

  8. Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae) of an Ecuadorian Mountain Forest Using DNA Barcoding

    OpenAIRE

    Thormann, Birthe; Ahrens, Dirk; Marín Armijos, Diego; Peters, Marcell K.; Wagner, Thomas; Wägele, Johann W

    2016-01-01

    Background Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient i...

  9. Characterization and chromosome location of satellite DNA in the leaf beetle Chrysolina americana (Coleoptera, Chrysomelidae).

    Science.gov (United States)

    Lorite, P; Palomeque, T; Garnería, I; Petitpierre, E

    2000-01-01

    This paper is the first record of the satellite DNA of the specialized phytophagous genus Chrysolina. The satellite DNA of Chrysolina americana is organized in a tandem repeat of monomers 189 bp long, has a A + T content of 59.6% and presents direct and inverted internal repeats. Restriction analysis of the total DNA with methylation sensitive enzymes suggests that this repetitive DNA is undermethylated. In siti hybridization with a biotinylated probe of the satellite DNA showed the pericentromeric localization of these sequences in all meiotic bivalents. The presence of this repetitive DNA in other species of the genus was also tested by Southern analysis. The results showed that this satellite DNA sequence is specific to the C. americana genome and has not been found in three other species of Chrysolina with a different choice of host plants than in the former. PMID:11678504

  10. Karyotype, heterochromatin distribution and meiosis of Zabrotes subfasciatus (Bohemann) (Coleoptera: Chrysomelidae, Bruchinae).

    Science.gov (United States)

    Corrêa, Ronan X; Pompolo, Sílvia G; Santos, Igor S; Silva, Janisete G; Costa, Marco A

    2008-01-01

    Zabrotes subfasciatus (Boh.) has been extensively studied in its agronomic and biochemical aspects due to its importance as a damaging insect to leguminous grains during storage. The few cytogenetic studies published on this species yielded conflicting results. In this study, the karyotype was analyzed in order to accurately describe the chromosome C-banding patterns and meiosis. The brain ganglion at the prepupa and the adult and pupal testes were analyzed. All individuals had 26 chromosomes in both brain ganglion and spermatogonic mitotic metaphases. These chromosomes were classified as follows: the 12th pair and the Y chromosome were telocentric; the X chromosome was acrocentric; the 4th and 5th pairs were submetacentric; and the remaining pairs were all metacentric. One of the members of the 5th pair presented a secondary constriction. All chromosomes presented pericentromeric heterochromatin. The large arms of the pairs 5, 9 and X presented heterochromatin. The X chromosome showed to be heteropyknotic throughout the prophase of the first meiotic division. The subphases of prophase I were atypical and meiosis II was rarely identified. Testes of all males showed a few cells; the bivalents were rod-like shaped in metaphase I. Karyological formulae were 2n = 24 + XX in females and 2n = 24 + XYp and either n = 12 + X or n = 12 + Y in males. PMID:19061039

  11. A REVISION OF THE PACHNEPHORUS FROM THE AFROTROPICAL REGION (COLEOPTERA, CHRYSOMELIDAE

    Directory of Open Access Journals (Sweden)

    Stefano Zoia

    2007-04-01

    Full Text Available A revision of the genus Pachnephorus Chevrolat, 1837 from the Afrotropical Region is given and a key to the species is provided. Types of all the previously known taxa have been studied and redescribed; 40 new taxa are described and illustrated: P. achardi n. sp. (Mali, P. aequatorianus n. sp. (Rep. Pop. Congo, P. aethiopicus n. sp. (Etiopia, P. baehri n. sp. (Namibia, P. balyi n. sp. (Angola, P. beharui n. sp. (Etiopia, P. bertiae n. sp. (Madagascar, P. bezdeki n. sp. (Rep. Pop. Congo, P. bracarumvestitus n. sp. (Rep. Pop. Congo, P. bryanti n. sp. (Mali, P. burgeoni n. sp. (Natal, P. camerun­ensis n. sp. (Camerun, P. cristiani n. sp. (Namibia, P. crocodilinus n. sp. (Zambia, P. daccordii n. sp. (Yemen, P. danielssoni n. sp. (Sierra Leone, P. danielssoni congoanus n. ssp. (Rep. Pop. Congo, P. demeyeri n. sp. (Rep. Pop. Congo, P. episternalis n. sp. (Madagascar, P. fabianae n. sp. (Congo, P. fasciatus occidentalis n. ssp. (Nigeria, P. gardinii n. sp. (Etiopia, P. gerstaeckeri n. sp. (Namibia, P. grobbelaarae n. sp. (South Africa, P. hajeki n. sp. (Madagascar, P. lopatini n. sp. (Senegal, P. malicus n. sp. (Mali, P. maroantsetranus n. sp. (Madagascar, P. medvedevi n. sp. (Zambia, P. mo­seykoi n. sp. (Chad, P. pacificus n. sp. (Central Afr. Rep., P. parentorum n. sp. (Ghana, P. poggii n. sp. (Somalia, P. regalini n. sp. (Zambia, P. rigatoi n. sp. (Kenya, P. sas­sii n. sp. (Guinea Bissau, P. shuteae n. sp. (Rep. South Africa, P. sprecherae n. sp. (Madagascar, P. uhligi n. sp. (Namibia, P. willersi n. sp. (Namibia. The lectoypes of P. conspersus Gerstaecker, 1871, P. senegalensis Achard, 1914, P. latior Pic, 1921 and P. testaceipes Fairmaire, 1880 are designated. A new synonymy (P. costatus Achard, 1914 n. syn. of P. torridus Baly, 1878 and a nomenclatural change (Mecistes lineatus (Pic, 1921 n. comb. for Pachnephorus lineatus Pic, 1921 are proposed; the Lectotypes of M. lineatus and of M. flavipes (Gerstaecker, 1855 are designated.

  12. First-instar western corn rootworm (Coleoptera: chrysomelidae) response to carbon dioxide

    Energy Technology Data Exchange (ETDEWEB)

    Strnad, S.P.; Bergman, M.K.; Fulton, W.C.

    1986-08-01

    Responses of first-instar western corn rootworm to CO/sub 2/ and N/sub 2/ gas gradients were studied in a laboratory test arena. Number of larvae reaching the gas source, number of turns toward and away from the gas source, larval velocity, and number of turns per cm traveled were recorded. Larvae exhibited a positive chemotactic response to CO/sub 2/ but not N/sub 2/ or air. There was no indication that a kinesis of any type was involved because velocities and turning rates were not significantly different among treatments. Results indicate that newly hatched larve may use CO/sub 2/ to locate corn roots.

  13. The Temporal and Spatial Invasion Genetics of the Western Corn Rootworm (Coleoptera: Chrysomelidae in Southern Europe.

    Directory of Open Access Journals (Sweden)

    Darija Lemic

    Full Text Available This study describes the genetics of the western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte in southern Europe during the introduction (1996-2001 and establishment/spread (2002-2011 phases of its invasion. The Diabrotica microsatellite core-set was used to perform traditional population genetics analyses. Our results indicated that during the introduction phase genetic diversity and population genetic structure were lower overall as compared to the establishment/spread phase. Unusually high genetic differentiation was found between the Italy and southern Europe comparisons, including high differentiation between Italian populations separated by a short distance during the establishment/spread phase. STRUCTURE analysis revealed two genetic clusters during the introduction phase and two genetic clusters during the establishment/spread phase. However, bottlenecked populations were only detected during the invasion phase. A small but significant isolation by distance effect was noted in both phases. Serbia was the geographic source of WCR to Croatia and Hungary in the introduction phase, while the United States of America was the possible source of WCR to Italy in 2001. These introductory populations were the subsequent source of individuals sampled during the establishment/spread phase. Repeated introductions and admixture events in southern Europe may have resulted in genetically diverse WCR populations that have attained 83% of all known alleles worldwide.

  14. Comparative analysis of microbial diversity in Longitarsus flea beetles (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Kelley, Scott T; Dobler, Susanne

    2011-05-01

    Herbivorous beetles comprise a significant fraction of eukaryotic biodiversity and their plant-feeding adaptations make them notorious agricultural pests. Despite more than a century of research on their ecology and evolution, we know little about the diversity and function of their symbiotic microbial communities. Recent culture-independent molecular studies have shown that insects possess diverse gut microbial communities that appear critical for their survival. In this study, we combined culture-independent methods and high-throughput sequencing strategies to perform a comparative analysis of Longitarsus flea-beetles microbial community diversity (MCD). This genus of beetle herbivores contains host plant specialists and generalists that feed on a diverse array of toxic plants. Using a deep-sequencing approach, we characterized the MCD of eleven Longitarsus species across the genus, several of which represented independent shifts to the same host plant families. Database comparisons found that Longitarsus-associated microbes came from two habitat types: insect guts and the soil rhizosphere. Statistical clustering of the Longitarsus microbial communities found little correlation with the beetle phylogeny, and uncovered discrepancies between bacterial communities extracted directly from beetles and those from frass. A Principal Coordinates Analysis also found some correspondence between beetle MCD and host plant family. Collectively, our data suggest that environmental factors play a dominant role in shaping Longitarsus MCD and that the root-feeding beetle larvae of these insects are inoculated by soil rhizosphere microbes. Future studies will investigate MCD of select Longitarsus species across their geographic ranges and explore the connection between the soil rhizosphere and the beetle MCD. PMID:20844936

  15. New genera and species of leaf beetles (Coleoptera: Chrysomelidae) from China and South Korea

    Science.gov (United States)

    Two new genera from China (Taumaceroides Lopatin and Yunnaniata Lopatin) and 11 new species (Smaragdina quadrimaculata Lopatin, Smaragdina oblongum Lopatin, Hyphaenia volkovitshi Lopatin, Arthrotus daliensis Lopatin, Taumaceroides sinicus Lopatin, Yunnaniata konstantinovi Lopatin, Calomicrus yunnanu...

  16. A REVISION OF THE PACHNEPHORUS FROM THE AFROTROPICAL REGION (COLEOPTERA, CHRYSOMELIDAE)

    OpenAIRE

    Stefano Zoia

    2007-01-01

    A revision of the genus Pachnephorus Chevrolat, 1837 from the Afrotropical Region is given and a key to the species is provided. Types of all the previously known taxa have been studied and redescribed; 40 new taxa are described and illustrated: P. achardi n. sp. (Mali), P. aequatorianus n. sp. (Rep. Pop. Congo), P. aethiopicus n. sp. (Etiopia), P. baehri n. sp. (Namibia), P. balyi n. sp. (Angola), P. beharui n. sp. (Etiopia), P. bertiae n. sp. (Madagascar), P. bezdeki n. sp. (Rep. Pop. Congo...

  17. Karyotype, heterochromatin distribution and meiosis of Zabrotes subfasciatus (Bohemann) (Coleoptera: Chrysomelidae, Bruchinae)

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Ronan X.; Santos, Igor S.; Silva, Janisete G.; Costa, Marco A. [Universidade Estadual de Santa Cruz, Ilheus, BA (Brazil). Dept. de Ciencias Biologicas; Pompolo, Silvia G. [Universidade Federal de Vicosa, MG (Brazil). Dept. de Biologia Geral

    2008-09-15

    Zabrotes subfasciatus (Boh.) has been extensively studied in its agronomic and biochemical aspects due to its importance as a damaging insect to leguminous grains during storage. The few cytogenetic studies published on this species yielded conflicting results. In this study, the karyotype was analyzed in order to accurately describe the chromosome C-banding patterns and meiosis. The brain ganglion at the pre pupa and the adult and pupal testes were analyzed. All individuals had 26 chromosomes in both brain ganglion and spermatogonic mitotic metaphases. These chromosomes were classified as follows: the 12{sup th} pair and the Y chromosome were telocentric; the X chromosome was acrocentric; the 4{sup th} and 5{sup th} pairs were sub metacentric; and the remaining pairs were all metacentric. One of the members of the 5{sup th} pair presented a secondary constriction. All chromosomes presented pericentromeric heterochromatin. The large arms of the pairs 5, 9 and X presented heterochromatin. The X chromosome showed to be heteropyknotic throughout the prophase of the fi rst meiotic division. The sub phases of prophase I were atypical and meiosis II was rarely identified. Testes of all males showed a few cells; the bivalents were rod-like shaped in metaphase I. Karyological formulae were 2n = 24 + XX in females and 2n = 24 + XYp and either n = 12 + X or n = 12 + Y in males. (author)

  18. Sexual contact influences orientation to plant attractant in Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Chemical signals emitted by insects and their hosts are important for sexual communication and feeding. Plant volatiles facilitate the location of suitable hosts for feeding and oviposition, and may moderate responses to sex and aggregation pheromones. While mating has been shown to moderate behav...

  19. Susceptibility of brassicaceous plants to feeding by flea beetles, Phyllotreta spp. (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Soroka, Juliana; Grenkow, Larry

    2013-12-01

    Crucifer-feeding flea beetles, Phyllotreta spp., are chronic insect pests in Canadian prairie canola production. Multiple laboratory and field feeding bioassays were conducted to determine the susceptibility of a wide range of crucifer species, cultivars, and accessions to feeding by flea beetles with the goal of discovering sources of resistant germplasm. In 62 bioassays of 218 entries, no consistent decreased feeding by flea beetles was seen on any entries of Brassica carinata A. Braun, Brassica juncea (L.) Czern., Brassica napus L., or Brassica rapa L. There was reduced feeding on condiment mustard Sinapis alba L. lines but not on canola-quality lines with reduced amounts of glucosinolates, which were fed on at levels equal to B. napus. Analyses of glucosinolate content found decreased quantities of hydroxybenzyl and butyl glucosinolates in preferred canola-quality S. alba lines and increased levels of hydroxybutenyl glucosinolates compared with levels in condiment S. alba lines. Eruca sativa Mill. was an excellent flea beetle host; Camelina sativa (L.) Crantz lines experienced little feeding. Lines of Crambe abyssinica Hochst. ex R. E. Fries and Crambe hispanica L. had reduced feeding levels compared with Brassica entries, but Crambe glabrata DC did not. The results indicate possible sources of resistance to Phyllotreta flea beetles, while highlighting the complicated roles that glucosinolates may play in Phyllotreta host preference. PMID:24498758

  20. Effects of seed type and bruchid genotype on the performance and oviposition behavior of Zabrotes subfasciatus (Coleoptera: Bruchidae)

    Institute of Scientific and Technical Information of China (English)

    ERICK D. M. CAMPAN; BETTY BENREY

    2006-01-01

    The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae), was determined by using wild and cultivated seeds of the genus Phaseolus. Results showed that the quality of the host plant affected the performance and the oviposition behavior of female beetles. Overall, bruchid performance was higher on cultivated seeds than on wild seeds. It was also found that the oviposition behavior and the performance of their offspring differed between females that originated from wild versus cultivated seeds. We also demonstrated the importance of a genetic component in bruchid performance: longevity, fecundity, larval development, adult For example, on the same host type, some females laid twice as many eggs as females from Thus, the performance and behavior of Z. subfasciatus are not only affected by environmental factors such as the quality of the seeds on which they develop, but also have a genetic basis which can counterbalance a less suitable quality of the host plant. For a crop pest such as Z.subfasciatus, its ability to survive and adapt on host plants of differing quality may be an important attribute to consider for pest management.

  1. The complete mitochondrial genome of Epicauta chinensis (Coleoptera: Meloidae) and phylogenetic analysis among Coleopteran insects.

    Science.gov (United States)

    Du, Chao; He, Shilin; Song, Xuhao; Liao, Qi; Zhang, Xiuyue; Yue, Bisong

    2016-03-10

    The blister beetle is an important resource insect due to its defensive substance cantharidin, which was widely used in pharmacology and plant protection. We determined the complete mitochondrial genome of Epicauta chinensis Laporte (Coleoptera: Tenebrionoidae: Meloidae). The circular genome is 15,717 bp long, encoding 13 protein-coding genes (PCGs), two ribosomal RNAs and 22 tRNAs and containing a A+T-rich region with gene arrangement identical to other Coleopteran species. Twelve PCGs start with typical ATN codon, while ATP8 gene initiate with GTT for first report in Insecta. All PCGs terminate with conventional stop codon TAA or TAG. All tRNAs in E. chinensis are predicted to fold into typical cloverleaf secondary structure, except tRNA-Ser(AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. The secondary structure of lrRNA and srRNA comprises 48 helices and 32 helices respectively. The 1101 bp A+T-rich region contains a 15 bp poly-T stretch and microsatellite-like repeats rather than large tandem repetitive sequences. Phylogenetic analysis, based on 13 PCGs of 45 Coleopteran species, show that E. chinensis grouped with Tenebrionidae species. It also support the topology of (((Chrysomelidae+Curculionoidea)+(Cucujoidea+Cleroidea))+Tenebrionoidea) within Cucujiformia. PMID:26707213

  2. Relationships of abscised cotton fruit to boll weevil (Coleoptera: Curculionidae) feeding, oviposition, and development.

    Science.gov (United States)

    Showler, Allan T

    2008-02-01

    Abscised cotton, Gossypium hirsutum L., fruit in field plots planted at different times were examined to assess adult boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), use of squares and bolls during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Although boll abscission is not necessarily related to infestation, generally more bolls abscised than squares and abundances of fallen bolls were not related to the planting date treatments. During 2003, fallen squares were most abundant in the late-planted treatment. Although large squares (5.5-8-mm-diameter) on the plant are preferred for boll weevil oviposition, diameter of abscised squares is not a reliable measurement because of shrinkage resulting from desiccation and larval feeding. Fallen feeding-punctured squares and bolls were most abundant in late plantings but differences between fallen feeding-punctured squares versus fallen feeding-punctured bolls were found in only one treatment in 2003. During the same year, fallen oviposition-punctured squares were more numerous in the late-planted treatment than in the earlier treatments. Treatment effects were not found on numbers of oviposition-punctured bolls, but fallen oviposition-punctured squares were more common than bolls in the late-planted treatment compared with earlier treatments each year. Dead weevil eggs, larvae, and pupae inside fallen fruit were few and planting date treatment effects were not detected. Living third instars and pupae were more abundant in fallen squares of the late-planted treatment than in the earlier treatments and bolls of all three treatments. This study shows that fallen squares in late-planted cotton contribute more to adult boll weevil populations than bolls, or squares of earlier plantings. PMID:18330118

  3. A variabilidade espacial das famílias de Coleoptera (Insecta entre fragmentos de Floresta Ombrófila Mista Montana (Bioma Araucária e plantação de Pinus elliottii Engelmann, no Parque Ecológico Vivat Floresta, Tijucas do Sul, Paraná, Brasil Spatial variability of Coleoptera (Insecta families between a Montane Ombrophilous Mixed Forest (Bioma Araucaria and Pinus elliottii Engelmann plantation fragments, in the Parque Ecológico Vivat Floresta, Tijucas do Sul, Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2006-12-01

    Full Text Available Várias questões sobre a biodiversidade vêm sendo levantadas nas últimas décadas. Uma delas é o papel que as plantações florestais exóticas monoculturais desempenham na manutenção da fauna nativa, principalmente de insetos. Estudos têm demonstrado que os Coleoptera são sensíveis a variações florísticas e estruturais, em pequena escala espacial. Para analisar as possíveis diferenças na diversidade de Coleoptera entre um ambiente de floresta natural e uma plantação florestal exótica monocultural foi desenvolvido um inventário no Parque Ecológico Vivat Floresta, Tijucas do Sul, Paraná. Para tanto, durante 52 semanas (agosto de 2004 a julho de 2005, seis armadilhas malaise foram dispostas ao longo de um transecto através de dois ambientes adjacentes, com diferentes condições florísticas: três em fragmento da floresta natural (Floresta Ombrófila Mista e três na plantação de Pinus elliottii exótico. Neste primeiro estudo, as comunidades de Coleoptera foram analisadas com base na abundância e na riqueza das famílias. Foram coletados 12397 exemplares de 57 famílias. A abundância foi maior na floresta natural, decrescendo do interior desta para o interior da plantação de pinus. O ecótono - borda da floresta natural/borda da plantação de pinus - foi o mais rico em famílias. Como observado em inventários de outras localidades, os estudos apoiados em dados das famílias que se incluem nos primeiros 60% da abundância total de cada área, mostram os mesmos resultados quando são aplicados os dados de todas as famílias. Na plantação de pinus as famílias dominantes foram, pela ordem: Cerambycidae, Staphylinidae, Curculionidae, Nitidulidae, Lampyridae, Scolytidae, Chrysomelidae; na floresta natural: Chrysomelidae, Cerambycidae, Curculionidae, Lampyridae, Nitidulidae, Staphylinidae.An important question for biodiversity is what is the impact of monocultures of exotic forest trees on native fauna, especially

  4. A diversidade inventarial de Coleoptera (Insecta em uma paisagem antropizada do Bioma Araucária The inventory diversity of Coleoptera (Insecta of an anthropized landscape in the Biome Araucaria

    Directory of Open Access Journals (Sweden)

    Norma G. Ganho

    2005-12-01

    Full Text Available Como parte do Projeto PROVIVE, foram analisadas a riqueza de espécies, a composição taxonômica, a proporção de espécies raras e a constância taxonômica ao nível de família relacionada à riqueza de espécies, em comunidades de Coleoptera, em Vila Velha, Ponta Grossa, Paraná. Os dados foram obtidos a partir de coletas através de armadilha malaise no estrato do sub-bosque de cinco áreas com diferentes graus de intervenção antrópica, de setembro de 1999 a agosto de 2000. As 52 semanas de amostragem nas cinco áreas resultaram na coleta de 10.822 indivíduos de 1659 espécies. Todas as áreas apresentaram alta riqueza de espécies e diversidade, como indicado por vários índices. A área em estágio mais avançado de sucessão vegetal foi menos rica do que aquelas em estágio inicial/intermediária. De acordo com diferentes estimadores de riqueza de espécies, o número de espécies coletadas poderia aumentar de 22-123% com o aumento do esforço de coleta. As áreas menos conservadas foram mais ricas em espécies raras ("singletons", "doubletons" e únicas do que as mais conservadas. Nas cinco áreas houve uma constância taxonômica entre as famílias mais ricas (Curculionidae, Chrysomelidae, Cerambycidae, Staphylinidae, Mordelidae, Elateridae, Scarabaeidae, Coccinellidae e Tenebrionidae envolvendo 60% do total de espécies, como observado para a abundância de indivíduos. A existência de um padrão de constância taxonômica de famílias, quando considerados 60% da riqueza de espécies e/ou de abundância de indivíduos por local, poderá tornar mais fácil e rápido o estudo de comunidades de Coleoptera, habilitando a ordem a ser um táxon indicador de condições ambientais de áreas florestadas.The species richness, taxonomic composition, rare species, and taxonomic constancy at family level were studied in communities of Coleoptera in Vila Velha, Ponta Grossa, Paraná, as part of PROVIVE project. The data were gathered

  5. Attraction of Astylus variegatus (Germ. (Coleoptera: Melyridae by volatile floral attractants Atração de Astylus variegatus (Germ. (Coleoptera: Melyridae por atraentes florais voláteis

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    Maurício Ursi Ventura

    2007-06-01

    Full Text Available The beetle Astylus variegatus (Germ. (Coleoptera: Melyridae is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L. fields. Traps originally designed to capture Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consisted of plastic bottles (2 L with 150 holes (5-mm diameter yellow gold painted and containing inside a plastic strip (3.5 ´ 25 cm with Lagenaria vulgaris (L. powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap, 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.O besouro Astylus variegatus (Germ. (Coleoptera: Melyridae é freqüentemente encontrado em flores onde se alimenta de pólen. Respostas de A. variegatus a atraentes voláteis florais foram estudadas em campos de feijão, Phaseolus vulgaris L. Armadilhas, originalmente desenvolvidas para capturar Diabrotica speciosa (Germ. (Coleoptera: Chrysomelidae, consistiram de garrafas plástica (2 L com 150 perfurações (5 mm de diâmetro pintadas com tinta amarelo ouro contendo no seu interior uma fita plástica (3,5 ´ 25,0 cm com pó seco de frutos de Lagenaria vulgaris (L. (0,28% de cucurbitacina B estimulante alimentar e arrestante para diabrotic

  6. Virulence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae for the control of Diabrotica speciosa germar (coleoptera: chrysomelidae Virulência de nematoides entomopatogênicos (Rhabditida: Steinernematidae e Heterorhabditidae para o controle de Diabrotica speciosa germar (coleoptera: chrysomelidae

    Directory of Open Access Journals (Sweden)

    Viviane Santos

    2011-12-01

    Full Text Available Entomopathogenic nematodes (EPNs are used in biological control of soil insects and show promise in the control of D. speciosa. The objective of this work was to evaluate the potential of native and exotic entomopathogenic nematode isolates in the control of D. speciosa under laboratory and greenhouse conditions. Results showed that all of EPNs caused larval mortality. The most virulent were Heterorhabditis sp. RSC01 (94%, Steinernema glaseri (84%, Heterorhabditis sp. JPM04 (82% and Heterorhabditis amazonensis RSC05 (78%. There was no effect of the Heterorhabditis sp. RSC01 and S. glaseri isolates on eggs. The maximum mortality of D. speciosa larvae by Heterorhabditis sp. RSC01 was observed at a concentration of 300 IJ/ insect, while by S. glaseri observed the highest mortality at the concentration of 200 IJ/ insect. The Heterorhabditis sp. RSC01 isolate caused over 80% pupal mortality at a concentration of 250 IJ/insect. The virulence of Heterorhabditis sp. RSC01 and S. glaseri was affected by temperature. The Heterorhabditis sp. RSC01 isolate caused reduction in larva survival under greenhouse conditions at all of the tested concentrations and there was no difference in mortality among different concentrations of infectid juveniles.Os nematóides entomopatogênicos são utilizados no controle biológico de pragas de solo, e são promissores para o controle de D. speciosa. Neste trabalho, objetivou-se avaliar o potencial de espécies nativas e exóticas de isolados de nematóides entomopatogênicos para o controle de D. speciosa, em condições de laboratório e de casa de vegetação. Verificou-se que todos os nematóides causaram mortalidade larval. Os mais virulentos foram Heterorhabditis sp. RSC01 (94%, Steinernema glaseri (84%, Heterorhabditis sp. JPM04 (82% e Heterorhabditis amazonensis RSC05 (78%. Não houve efeito dos isolados Heterorhabditis sp. RSC01 e S. glaseri em ovos. A mortalidade máxima de larvas de D. speciosa por Heterorhabditis sp. RSC01 foi observada na concentração de 300 JI / inseto, enquanto para S. glaseri a maior mortalidade foi obervada na concentração de 200 JI/ inseto. O isolado Heterorhabditis sp. RSC01 causou mais de 80% de mortalidade de pupas na concentração de 250 IJ/ inseto. A virulência de Heterorhabditis sp. RSC01 e S. glaseri foi afetada pela temperatura. O isolado Heterorhabditis sp. RSC01 causou redução na sobrevivência da larvas em casa de vegetação em todas as concentrações de juvenis infectantes testadas e não houve diferença na mortalidade entre os diferentes tratamentos.

  7. Immatures of Heterispa vinula (Erichson and Physocoryna scabra Guérin-Méneville (Coleoptera, Chrysomelidae, Cassidinae, Chalepini Imaturos de Heterispa vinula (Erichson e Physocoryna scabra Guérin (Coleoptera, Chrysomelidae, Cassidinae, Chalepini

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    Sônia A. Casari

    2004-12-01

    Full Text Available Larva and pupa of Heterispa vinula (Erichson, 1847 and larva of Physocorina scabra Guérin-Méneville, 1844 are described and illustrated. The material of H. vinula was collected feeding on Sida carpinifolia (L.f. K. Schum (Malvaceae in the city of São Paulo (Ipiranga and Mooca districts, and of P. scabra on acerola (Malpighia glabra L. (Malpighiacea in Cruz das Almas, Bahia. Larvae of genus Physocorina are described for the first time.Larva e pupa de Heterispa vinula (Erichson, 1847 e larva de Physocorina scabra Guérin-Méneville, 1844 são descritas e ilustradas. O material de H. vinula foi coletado em Sida carpinifolia (L.f. K. Schum (Malvaceae em São Paulo (Ipiranga e Mooca, e P. scabra em acerola (Malpighia glabra L. (Malpighiacea em Cruz das Almas, Bahia. Os imaturos do gênero Physocoryna são descritos pela primeira vez.

  8. Efeitos da terra diatomácea sobre Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae em batata inglesa Effects of diatomaceous earth on Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae in potato

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    Franscinely Aparecida Assis

    2011-06-01

    Full Text Available A espécie Diabrotica speciosa é um crisomelídeo responsável por causar danos consideráveis à batateira, o que torna indispensável seu controle com inseticidas para minimizar os prejuízos causados à cultura. Assim, objetivou-se, com este trabalho, avaliar os efeitos da terra diatomácea sobre o comportamento alimentar e a mortalidade de D. speciosa em batata inglesa em condições de laboratório. Adotou-se o DIC com três tratamentos e oito repetições, sendo: 1 - testemunha; 2 - TD polvilhada (0,5 g/vaso e 3 - TD pulverizada a 1%. As batateiras, cv. Emeraude, foram polvilhadas ou pulverizadas com TD, 30 dias após o plantio e, 24 horas após a aplicação, as plantas foram fornecidas aos insetos, sem e com chance de escolha. Houve diferença significativa entre os tratamentos, sendo a menor porcentagem de folíolos com injúrias observada em plantas que receberam a aplicação de TD pulverizada (teste sem chance de escolha e TD polvilhada ou pulverizada (teste com chance de escolha. Também houve redução do número de injúrias foliares às 24 e às 48 horas (TD polvilhada ou pulverizada e, às 72 horas, a redução foi observada somente com a aplicação da TD polvilhada. Com relação à ação inseticida da TD, foi verificado seu efeito após 48 horas, tanto via polvilhamento, quanto via pulverização. Assim, a aplicação de TD pode auxiliar no manejo de D. speciosa, contribuindo para conferir proteção às plantas de batata inglesa e aumentar a mortalidade desse inseto-praga.The species D. speciosa is a chrysomelid responsible for causing considerable damage on potato plant, making its control with insecticides essential to minimize the damage caused to this culture. The objective of this study was to evaluate the effects of diatomaceous earth (DE on feeding behavior and mortality of D. speciosa in potato under laboratory conditions. A completely randomized design was used with three treatments and eight replicates being: 1 - control; 2 - sprinkled DE (0.5 g/vase and 3 - 1% sprayed DE. The potato plants, cv. Emeraude, were sprinkled or sprayed with DE 30 days after the planting and 24 hours after the application, the plants were supplied to insects, with and without a chance of choice. There was significant difference among treatments with a lower leaflet with injury percentage observed in plants that received the application of powdered DE (tests without chance of choice and sprinkled or powdered DE (tests without chance of choice. There was also reduction of the number of leaf injuries at 24 and 48 hours (sprinkled or powdered DE and, at 72 hours, the reduction was only observed with the application of sprinkled DE. Regarding the insecticidal activity of TD, its effect was verified after 48 hours, through sprinkling or spraying. As a result, the application of DE may help in the management of D. speciosa, contributing to provide protection to potato plants and to increase the mortality of this insect pest.

  9. Adopting Bacteria in Order to Adapt to Water—How Reed Beetles Colonized the Wetlands (Coleoptera, Chrysomelidae, Donaciinae

    Directory of Open Access Journals (Sweden)

    Birgit Kleinschmidt

    2011-12-01

    Full Text Available The present paper reviews the biology of reed beetles (Donaciinae, presents experimental data on the role of specific symbiotic bacteria, and describes a molecular method for the detection of those bacteria. Reed beetles are herbivores living on wetland plants, each species being mono- or oligo-phagous. They lay their eggs on the host plant and the larvae live underwater in the sediment attached to its roots. The larvae pupate there in a water-tight cocoon, which they build using a secretion that is produced by symbiotic bacteria. The bacteria are located in four blind sacs at the foregut of the larvae; in (female adults they colonize two out of the six Malpighian tubules. Tetracycline treatment of larvae reduced their pupation rate, although the bacteria could not be fully eliminated. When the small amount of bacterial mass attached to eggs was experimentally removed before hatching, symbiont free larvae resulted, showing the external transmission of the bacteria to the offspring. Specific primers were designed to detect the bacteria, and to confirm their absence in manipulated larvae. The pupation underwater enabled the reed beetles to permanently colonize the wetlands and to diversify in this habitat underexploited by herbivorous insects (adaptive radiation.

  10. Assessment of fitness costs in Cry3Bb1 resistant and susceptible western corn rootworm (Coleoptera:Chrysomelidae) laboratory colonies

    Science.gov (United States)

    Maize production in the United States is dominated by plants genetically modified with transgenes from Bacillus thuringiensis (Bt). Varieties of Bt maize expressing Cry3Bb endotoxins that specifically target corn rootworms (genus Diabrotica) have proven highly efficacious. Howeve...

  11. Efficiency of vegetable extracts for the control of Diabrotica speciosa (Germar, 1824) (Coleoptera: Chrysomelidae), in the laboratory

    OpenAIRE

    Flávio Roberto Mello Garcia; Patricia Migliorini; Junir Antonio Lutinski

    2010-01-01

    Vulgarly known as “vaquinha”, Diabrotica speciosa (Germar, 1824) spread itself to the majority of Brazilian states, and it became distinguished as one of the most serious pests affecting beans and maize. The aim of this study was to evaluate the insecticidal activity of the crude watery extracts of nine vegetable species on “vaquinha” adults. The laboratory experiment was carried out in completely randomized delineation, with ten treatments and four repetitions. For such, a bottle was used, c...

  12. The Effect of Western Corn Rootworm (Coleoptera: Chrysomelidae) and Water Deficit on Maize Performance Under Controlled Conditions.

    Science.gov (United States)

    Mahmoud, M A B; Sharp, R E; Oliver, M J; Finke, D L; Ellersieck, M R; Hibbard, B E

    2016-04-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte, is the most important insect of maize, Zea mays L., but knowledge of its interaction with water deficit on maize production is lacking. A series of greenhouse experiments using three infestation levels of the western corn rootworm, D. virgifera virgifera, under well-watered, moderately dry, and very dry soil moisture levels were conducted to quantify the interaction of western corn rootworm and soil water deficit on B73×Mo17 maize growth and physiology. Three separate experiments were conducted. Soil moisture regimes were initiated 30 d postplanting for experiments using neonate and second-instar larvae and 30 d postinfestation in the experiment using eggs. In the neonate and second-instar experiments, there were no significant differences among western corn rootworm levels in their effects on leaf water potential, shoot dry weight, and root dry weight. The interaction of western corn rootworm and soil moisture significantly impacted the larval recovery in the neonate experiment, but no other significant interactions were documented between soil moisture levels and rootworm infestation levels. Overall, the results indicate that under the conditions of these experiments, the effect of water deficit was much greater on plants than the effect of western corn rootworm and that the interactions between water deficit and western corn rootworm levels minimally affected the measured parameters of plant performance. PMID:26896532

  13. Phylogenetic information reveals the peculiarity of Caryedon serratus (Coleoptera, Chrysomelidae, Bruchinae) feeding on Cassia sieberiana DC (Caesalpinioideae)

    OpenAIRE

    Sembène, M.; Kébé, K.; Delobel, A.; Rasplus, Jean Yves

    2010-01-01

    Studies over the past 20 years on natural populations indicated that sympatric speciation may be far more common and widespread among plants and animals than previously thought. By using molecular phylogenetics (on a combined data set of two genes), the relationships between Caryedon serratus native forms and forms feeding on groundnut was investigated. The specific objectives were to clarify the taxonomic status of C. serratus feeding on Cassia sieberiana DC. Morphological analysis was condu...

  14. Efficiency of vegetable extracts for the control of Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae, in the laboratory

    Directory of Open Access Journals (Sweden)

    Flávio Roberto de Mello Garcia

    2010-03-01

    Full Text Available Vulgarly known as “vaquinha”, Diabrotica speciosa (Germar, 1824 spread itself to the majority of Brazilian states, and it became distinguished as one of the most serious pests affecting beans and maize. The aim of this study was to evaluate the insecticidal activity of the crude watery extracts of nine vegetable species on “vaquinha” adults. The laboratory experiment was carried out in completely randomized delineation, with ten treatments and four repetitions. For such, a bottle was used, containing five insect specimens and a common bean leaf (Phaseolus vulgaris Linnaeus previously immersed in the extract, covered with a clipping of porous cloth and fixed by a rubber band. The evaluated variable was the number of surviving D. speciosa specimens. The treatments consisted of salvia (Salvia officinalis Linnaeus, cravo (Eugenia caryophyllata Thunb, moscada nut (Myristica fragans Houtt, cinamomo (Melia azedarach Linnaeus, timbo (Ateleia glazioveana Baill, eucalyptus (Eucalyptus citriodora Hook, cinnamon (Cinnamomum zeylanicum Blume, figueira (Ficus microcarpa Linnaeus f., rosemary (Rosmarinus officinalis Linnaeus and control (distilled water alone. The evaluations of survival were carried out every 24 hours over a period of 10 days. For the live specimen number, two-way analysis of variance (10 extracts x 11 times after application was used. The averages were grouped by the Duncan test on the level of 5% of probability. The most efficient extracts were timbo, moscada nut and cinamomo, with efficiency percentages varying between 80.4% and 100%.

  15. Emergence and Abundance of Western Corn Rootworm (Coleoptera: Chrysomelidae) in Bt Cornfields With Structured and Seed Blend Refuges.

    Science.gov (United States)

    Hughson, Sarah A; Spencer, Joseph L

    2015-02-01

    To slow evolution of western corn rootworm (Diabrotica virgifera virgifera LeConte) resistance to Bt (Bacillus thuringiensis Berliner) corn hybrids, non-Bt "refuges" must be planted within or adjacent to Bt cornfields, allowing susceptible insects to develop without exposure to Bt toxins. Bt-susceptible adults from refuges are expected to find and mate with resistant adults that have emerged from Bt corn, reducing the likelihood that Bt-resistant offspring are produced. The spatial and temporal distribution of adults in four refuge treatments (20, 5, and 0% structured refuges and 5% seed blend) and adjacent soybean fields was compared from 2010 to 2012. Adult emergence (adults/trap/day) from refuge corn in structured refuge treatments was greater than that from Bt corn, except during the post-pollination period of corn phenology when emergence from refuge and Bt plants was often the same. Abundance of free-moving adults was greatest in and near refuge rows in structured refuge treatments during vegetative and pollination periods. By post-pollination, adult abundance became evenly distributed. In contrast, adult abundance in 5% seed blends and 0% refuges was evenly distributed, or nearly so, across plots throughout the season. The persistent concentration of adults in refuge rows suggests that structured refuge configurations may not facilitate the expected mixing of adults from refuge and Bt corn. Seed blends produce uniform distributions of adults across the field that may facilitate mating between Bt and refuge adults and ultimately delay the evolution of Bt resistance. PMID:26470111

  16. Inhibition of seed germination by extracts of bitter Hawkesbury watermelon containing cucurbitacin, a feeding stimulant for corn rootworm (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Martin, Phyllis A W; Blackburn, Michael

    2003-04-01

    Cucurbitacins are feeding stimulants for corn rootworm used in baits to control the adults of this insect pest. Corn rootworm larvae also feed compulsively on cucurbitacins. Cucurbitacins are reported to be gibberellin antagonists that may preclude their use as seed treatments for these soil-dwelling insects. The crude extract of a bitter Hawkesbury watermelon containing cucurbitacin E-glycoside significantly inhibited germination of watermelon, squash, and tomato seeds. Although the germination of corn seed was not significantly inhibited, root elongation was inhibited by crude extracts, but not by high-performance liquid chromatography-purified cucurbitacin E-glycoside. Therefore, the effects of the major components in the bitter watermelon extract (e.g., sugars) on seed germination and root elongation were determined. Pure sugars (glucose and fructose), at concentrations found in watermelon extract, mimicked the inhibition of seed germination and root elongation seen with the crude bitter Hawkesbury watermelon extract. Removal of these sugars may be necessary to use this extract as a bait for corn rootworm larvae as a seed or root treatment. PMID:14994812

  17. ANTIPREDATOR BEHAVIOR OF SPOTTED CUCUMBER BEETLES (COLEOPTERA: CHRYSOMELIDAE) IN RESPONSE TO PREDATORS THAT POSE VARYING RISK. (R826099)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  18. Effects of pollutant accumulation by the invasive weed saltcedar (Tamarix ramosissima) on the biological control agent Diorhabda elongata (Coleoptera: Chrysomelidae)

    International Nuclear Information System (INIS)

    Hydroponic greenhouse studies were used to investigate the effect of four anthropogenic pollutants (perchlorate (ClO4-), selenium (Se), manganese (Mn), and hexavalent chromium (Cr (VI))) on the biological control agent Diorhabda elongata Brulle. Contaminant concentrations were quantified for experimental Tamarix ramosissima Ledab. plants and D. elongata beetles. Growth of larvae was significantly reduced by Se contamination, but was not affected by the presence of perchlorate, Mn, or Cr (VI). All of the contaminants were transferred from plants to D. elongata beetles. Only Cr (VI) was accumulated at greater levels in beetles than in their food. Because T. ramosissima grows in disturbed areas, acquires salts readily, and utilizes groundwater, this plant is likely to accumulate anthropogenic pollutants in contaminated areas. This study is one of the first to investigate the potential of an anthropogenic pollutant to influence a weed biological control system. - The presence of Se, but not perchlorate, Mn, or Cr (VI), in foliage of the invasive weed saltcedar was shown to reduce growth of the biological control agent Diorhabda elongata

  19. Characterization and evolutionary dynamics of a complex family of satellite DNA in the leaf beetle Chrysolina carnifex (Coleoptera, Chrysomelidae).

    Science.gov (United States)

    Palomeque, Teresa; Muñoz-López, Martín; Carrillo, José A; Lorite, Pedro

    2005-01-01

    The present study characterizes the complex satellite DNA from the specialized phytophagous beetle species Chrysolina carnifex. The satellite DNA is formed by six monomer types, partially homologous but having diverged enough to be separate on the phylogenetic trees, since each monomer type is located on a different branch, having statistically significant bootstrap values. Its analysis suggests a common evolutionary origin of all monomers from the same 211-bp sequence mainly by means of base-substitution mutations evolutionarily fixed to each monomer type and duplications and/or deletions of pre-existing segments in the 211-bp sequence. The analysis of the sequences and Southern hybridizations suggest that the monomers are organized in three types of repeats: monomers (211-bp) and higher-order repeats in the form of dimers (477-bp) or even trimers (633-bp). These repetitive units are not isolated from others, and do not present the pattern characteristic for the regular tandem arrangement of satellite DNA. In-situ hybridization with biotinylated probes corresponding to the three types of repeats showed the pericentromeric location of these sequences in all meiotic bivalents, coinciding with the heterochromatic blocks revealed by C-banding, indicating in addition that each type of repeat is neither isolated from others nor located in specific chromosomes but rather that they are intermixed in the heterochromatic regions. The presence of this repetitive DNA in C. haemoptera, C. bankii and C. americana was also tested by Southern analysis. The results show that this satellite DNA sequence is specific to the C. carnifex genome but has not been found in three other species of Chrysolina occupying similar or different host plants. PMID:16331411

  20. Global proteome changes in larvae of Callosobruchus maculatus Coleoptera:Chrysomelidae:Bruchinae) following ingestion of a cysteine proteinase inhibitor

    DEFF Research Database (Denmark)

    Nogueira, Fábio C S; Silva, Carlos P; Alexandre, Daniel;

    2012-01-01

    The seed-feeding beetle Callosobruchus maculatus is an important cowpea pest (Vigna unguiculata) as well as an interesting model to study insect digestive physiology. The larvae of C. maculatus rely on cysteine and aspartic peptidases to digest proteins in their diet. In this work, the global...

  1. Significance of colour polymorphism in mountain populations of abundant leaf beetles (Coleoptera, Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Mikhailov, Y. E.

    2001-12-01

    Full Text Available Leaf beetles, especially common and abundant species, play significant role in the flow of biomass and energy in alpine ecosystems. They feed openly in the leafage of shrubs and herbs and their various metallic colourations or highly melanistic forms absorb solar radiation, promoting warming and decreasing penetration of UV. Therefore colour polymorphism is important for monitoring of status of exact populations. Polymorphism as ecologically selected variability serves for the most complete and effective use of environmental heterogeneity. That is why composition of morphs and its changes in space and time are especially sensitive. Eco-geographical reguliarities of colour polymorphism is shown for various mountain populations of Chrysomela lapponica, Gonioctena árctica and Oreina sulcata throughout their distribution area. These species together with related ones enable to make a sensitive network of biosensors for climate change monitoring in Holarctic region. [fr]
    Les Scarabées des feuilles deviennent spécialement communs, présentent de nombreuses espèces et sans doute peuvent jouer un rôle important sur le cycle de biomasse et le flux d'énergie des écosystèmes alpiens. Ces insectes mangent les feuilles des arbrisseaux ou des herbes et leurs colorations métalliques très variées ou leurs formes fortement mélaniques absorbent les radiations solaires; c'est comme cela qu'ils provoquent un réchauffement et arrivent à filtrer les rayons UV. En conséquence, le polymorphisme de couleurs se montre très important pour le monitoring de plusieurs populations concernées. Si nous considérons ce polymorphisme comme un type de variabilité sélectionnée écologiquement, il peut bien contribuer à une utilisation plus effective et complète de l'hétérogénéité environnementale. C'est pourquoi la composition des morphotypes et ses changements aussi bien dans l'espace que dans le temps deviennent très sensibles. Chez les populations montagnardes de Chrysomela lapponica, Gonioctena arcaica et Oreina sulcata et au long de leur aire de répartition ont été observées certaines régularités ecogéographiques du poly- morphisme couleur. Sans doute ces espèces et d'autres semblables pourraient aider à établir un réseau de bioindicateurs permettant un monitoring du changement climatique sur la région Holartique.

    [fr]
    Les Scarabées des feuilles deviennent spécialement communs, présentent de nombreuses espèces et sans doute peuvent jouer un rôle important sur le cycle de biomasse et le flux d'énergie des écosystèmes alpiens. Ces insectes mangent les feuilles des arbrisseaux ou des herbes et leurs colorations métalliques très variées ou leurs formes fortement mélaniques absorbent les radiations solaires; c'est comme cela qu'ils provoquent un réchauffement et arrivent à filtrer les rayons UV. En conséquence, le polymorphisme de couleurs se montre très important pour le monitoring de plusieurs populations concernées. Si nous considérons ce polymorphisme comme un type de variabilité sélectionnée écologiquement, il peut bien contribuer à une utilisation plus effective et complète de l'hétérogénéité environnementale. C'est pourquoi la composition des morphotypes et ses changements aussi bien dans l'espace que dans le temps deviennent très sensibles. Chez les populations montagnardes de Chrysomela lapponica, Gonioctena arcaica et Oreina sulcata et au long de leur aire de répartition ont été observées certaines régularités ecogéographiques du poly- morphisme couleur. Sans doute ces espèces et d'autres semblables pourraient aider à établir un réseau de bioindicateurs permettant un monitoring du changement climatique sur la région Holartique.
    [es]
    Los escarabajos de las hojas resultan especialmente comunes, presentan numerosas especies y sin duda juegan un papel destacado en el ciclo de biomasa o en el flujo de energía de los ecosistemas alpinos. Estos insectos se alimentan del follaje de arbustos o hierbas y sus variadas coloraciones metálicas o sus formas altamente melánicas absorben la radiación solar; por ese procedimiento provocan un calentamiento y filtran los rayos ultravioleta. Así, el polimorfismo de colores resulta importante para el seguimiento de unas poblaciones determinadas. Considerado dicho polimorfismo como variabilidad seleccionada ecológicamente, puede contribuir a una utilización más completa y efectiva de la heterogeneidad ambiental. Esa es la razón por la cual una composición de morfotipos y sus cambios en el espacio y en el tiempo residían especialmente sensibles. Ciertas regularidades ecogeográficas del polimorfismo de colores pueden apreciarse en varias poblaciones montanas de Chrysomela lapponica, Gonioctena arcaica y Oreina sulcata a lo largo de su área de distribución. Tales especies y otras relacionadas permitirían el establecimiento de una red de biosensores para el seguimiento del cambio climático en la región Holártica.

  2. Effects of pollutant accumulation by the invasive weed saltcedar (Tamarix ramosissima) on the biological control agent Diorhabda elongata (Coleoptera: Chrysomelidae)

    Energy Technology Data Exchange (ETDEWEB)

    Sorensen, Mary A. [Department of Entomology, University of California, Riverside, CA 92521 (United States)], E-mail: mary.sorensen@ucr.edu; Parker, David R. [Department of Environmental Science, University of California, Riverside, CA 92521 (United States); Trumble, John T. [Department of Entomology, University of California, Riverside, CA 92521 (United States)

    2009-02-15

    Hydroponic greenhouse studies were used to investigate the effect of four anthropogenic pollutants (perchlorate (ClO{sub 4}{sup -}), selenium (Se), manganese (Mn), and hexavalent chromium (Cr (VI))) on the biological control agent Diorhabda elongata Brulle. Contaminant concentrations were quantified for experimental Tamarix ramosissima Ledab. plants and D. elongata beetles. Growth of larvae was significantly reduced by Se contamination, but was not affected by the presence of perchlorate, Mn, or Cr (VI). All of the contaminants were transferred from plants to D. elongata beetles. Only Cr (VI) was accumulated at greater levels in beetles than in their food. Because T. ramosissima grows in disturbed areas, acquires salts readily, and utilizes groundwater, this plant is likely to accumulate anthropogenic pollutants in contaminated areas. This study is one of the first to investigate the potential of an anthropogenic pollutant to influence a weed biological control system. - The presence of Se, but not perchlorate, Mn, or Cr (VI), in foliage of the invasive weed saltcedar was shown to reduce growth of the biological control agent Diorhabda elongata.

  3. Influence of weather factors on seasonal population dynamics of Coelaenomenodera elaeidis (Coleoptera: Chrysomelidae and its natural enemies in NIFOR, Nigeria

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    T. I. Aneni

    2013-12-01

    Full Text Available The leaf miner (Coelaenomenodera elaeidis Mlk is the major pest of the oil palm. The seasonality of C. elaeidis, its natural enemies and their relationship with temperature, rainfall and relative humidity was observed between January 2009 and December 2010 at the main station of the Nigerian Institute for Oil Palm Research, Nigeria. Past leaf miner population estimates were obtained from NIFOR entomology division from 1976 - 1980. This study analyses temporal patterns in leaf miner abundance, and elucidates general patterns and factors influencing leaf miner abundance. Multiple linear regressions were used to analyze the relationship between abundance of leaf miner, its parasitoids and predators and the following climatic variables: maximum and minimum temperature (oC, rainfall (mm and relative humidity (%. For the analyses, climate variables from the month of collection (control variable or from the month before the collection (delayed variable were used. The abundance of leaf miner and predatory ants peaked in the dry season, while parasitoids were most abundant in the rainy season. Significant correlations (P is not great than 0.05 were found between leaf miner, its natural enemies and both control and delayed weather variables. For all years, maximum temperature was the most dominant variable for all the leaf miner stages. Significant correlations were found between leaf miner, its natural enemies and both control and delayed weather variables. This indicates that the weather variables at both the month of collection and with a delayed month in relation to collection is critical for pest-weather evaluation. This is important for leaf miner control. Temperature, rainfall and relative humidity had an effect on the population of C. elaeidis, and this effect was manifested primarily in seasonal fluctuations in oil palm agroecosystems. Dry season months with resultant higher temperatures recorded higher population of C. elaeidis.

  4. Sexual dimorphism of Colorado beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae in the west and northwest of Iran by geometric morphometric method

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    Rahim Abdolahi Mesbah

    2014-12-01

    Full Text Available The males and females of Colorado beetles do not reveal clear dimorphism and therefore they have high resemblance so that recognition of the sexes by simple eye is too difficult. In order to study sexual dimorphism in Colorado beetle, three geographical populations were collected from potato fields in Ardabil, Bahar and Hamedan regions by manual method and direct observation in the summer of 2012. Fore and hind wings were separated and 7 and 8 landmarks were orderly selected for the fore and hind wings at the end and angle of veins. Geometric coordinate of landmarks were converted to shape and size variables as comparison factors between the sexes. Wings relative variations were determined separately in male and female and it revealed variations of wing shape in evolutionary process. Multivariate analysis based on the results of regression of shape variables showed fore wing had allometry and hind wing had not allometry. Two way MANOVA analysis was conducted for observation of shape differences (base on average of shape variables and size differences. The analysis showed that there were significant differences in shape of fore wing between the sexes.

  5. Teste de especificidade hospedeira de Phaedon confinis (Coleoptera, Chrysomelidae, um potencial agente de biocontrole de Senecio brasiliensis (Asteraceae.

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    Julianne Milléo

    2011-07-01

    Abstract. Senecio brasiliensis (Spreng. Less when ingested by cattle and horses, the plant causes seneciosis, a serious poisoning. Due to the great financial losses to cattle ranchers, controlling the plant using insects has become attractive. Systematic survey efforts have revealed that Phaedon confinis Klug causes serious damage to the plant, and may be a great biocontrol agent. The object was to extend the tests of host specificity to 52 plants using 1st larval instar and adult chrysomelid bettles. The insects were submitted to “no-choice” and “multiple-choice” tests. The following results were obtained: “NO-CHOICE” L1 – 52 plants tested: null 90.39%; negligible damage 5.77%; light 1.92%; and normal in only S. brasiliensis 1.92%, where 31.67% of larvae obtained an adult phase. “NO-CHOICE” ADULTS – 46 plants. Null damage was recorded in 82.60%; 13.04% showed negligible damage; 2.17% light; 2.17% normal in S. brasiliensis. The chysomelids oviposited during observation days only on S. brasiliensis leaves. 615 eggs were oviposited with 73.01% viability. “MULTIPLE CHOICE” LARVAE – nine plants tested. 66.67% null; 11.11% weak; 11.11% negligible damage; and 11.11% normal in S. brasiliensis. The results indicate that the normal diet, oviposition, survival and development of P. confinis is restricted to S. brasiliensis and corroborates its potential as a biocontrol agent.

  6. Natural history of the tortoise beetle, Discomorpha (Discomorpha) biplagiata (Guérin) (Chrysomelidae: Cassidinae: Omocerini)

    OpenAIRE

    Flowers, R. Wills; Chaboo, Caroline S.

    2016-01-01

    The fi rst natural history account of Discomorpha (Discomorpha) biplagiata (Guérin) (Chrysomelidae: Cassidinae: Omocerini) from Ecuador is presented. Larvae and adults feed on the leaves and stems of Cordia hebeclada Johnst. (Boraginaceae), the fi rst documented host plant for the species. Oviposition and pupation also occur on this host. Young larvae suffered heavy predation, especially from Ectatomma sp. (Formicidae: Ponerinae) and Oplomus nr. marginalis (Pentatomidae: Asopinae).

  7. Recalibrated tree of leaf beetles (Chrysomelidae indicates independent diversification of angiosperms and their insect herbivores.

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    Jesús Gómez-Zurita

    Full Text Available BACKGROUND: The great diversity of the "Phytophaga" (weevils, longhorn beetles and leaf beetles has been attributed to their co-radiation with the angiosperms based on matching age estimates for both groups, but phylogenetic information and molecular clock calibrations remain insufficient for this conclusion. METHODOLOGY: A phylogenetic analysis of the leaf beetles (Chrysomelidae was conducted based on three partial ribosomal gene markers (mitochondrial rrnL, nuclear small and large subunit rRNA including over 3000 bp for 167 taxa representing most major chrysomelid lineages and outgroups. Molecular clock calibrations and confidence intervals were based on paleontological data from the oldest (K-T boundary leaf beetle fossil, ancient feeding traces ascribed to hispoid Cassidinae, and the vicariant split of Nearctic and Palearctic members of the Timarchini. PRINCIPAL FINDINGS: The origin of the Chrysomelidae was dated to 73-79 Mya (confidence interval 63-86 Mya, and most subfamilies were post-Cretaceous, consistent with the ages of all confirmed body fossils. Two major monocot feeding chrysomelid lineages formed widely separated clades, demonstrating independent colonization of this ancient (early Cretaceous angiosperm lineage. CONCLUSIONS: Previous calibrations proposing a much older origin of Chrysomelidae were not supported. Therefore, chrysomelid beetles likely radiated long after the origin of their host lineages and their diversification was driven by repeated radiaton on a pre-existing diverse resource, rather than ancient host associations.

  8. Elemental distribution in reproductive and neural organs of the Epilachna nylanderi (Coleoptera: Coccinellidae), a phytophage of nickel hyperaccumulator Berkheya coddii (Asterales: Asteraceae) by micro-PIXE.

    Science.gov (United States)

    Mesjasz-Przybyłowicz, Jolanta; Orłowska, Elżbieta; Augustyniak, Maria; Nakonieczny, Mirosław; Tarnawska, Monika; Przybyłowicz, Wojciech; Migula, Paweł

    2014-01-01

    The phenomenon of metal hyperaccumulation by plants is often explained by a pathogen or herbivore defense hypothesis. However, some insects feeding on metal hyperaccumulating plants are adapted to the high level of metals in plant tissues. Former studies on species that feed on the leaves of Berkheya coddii Roessler 1958 (Asteraceae), a nickel-hyperaccumulating plant, demonstrated several protective mechanisms involved in internal distribution, immobilization, and elimination of Ni from the midgut and Malpighian tubules. These species are mainly coleopterans, including the lady beetle, Epilachna nylanderi (Mulsant 1850) (Coleoptera: Coccinellidae), collected from the ultramafic ecosystem near Barberton in South Africa. By performing particle-induced X-ray emission microanalysis elemental microanalysis (PIXE), this study examined whether Ni may be harmful to internal body systems that decide on insect reactivity (central nervous system [CNS]), their reproduction, and the relationships between Ni and other micronutrients. Data on elemental distribution of nine selected elements in target organs of E. nylanderi were compared with the existing data for other insect species adapted to the excess of metals. Micro-PIXE maps of seven regions of the CNS showed Ni mainly in the neural connectives, while cerebral ganglia were better protected. Concentrations of other bivalent metals were lower than those of Ni. Testis, compared with other reproductive organs, showed low amounts of Ni. Zn was effectively regulated at physiological dietary levels. In insects exposed to excess dietary Zn, it was also accumulated in the reproductive organs. Comparison of E. nylanderii with other insects that ingest hyperaccumulating plants, especially chrysomelid Chrysolina clathrata (Clark) (Coleoptera: Chrysomelidae), showed lower protection of the CNS and reproductive organs. PMID:25399425

  9. Coleoptera Associated with Decaying Wood in a Tropical Deciduous Forest.

    Science.gov (United States)

    Muñoz-López, N Z; Andrés-Hernández, A R; Carrillo-Ruiz, H; Rivas-Arancibia, S P

    2016-08-01

    Coleoptera is the largest and diverse group of organisms, but few studies are dedicated to determine the diversity and feeding guilds of saproxylic Coleoptera. We demonstrate the diversity, abundance, feeding guilds, and succession process of Coleoptera associated with decaying wood in a tropical deciduous forest in the Mixteca Poblana, Mexico. Decaying wood was sampled and classified into four stages of decay, and the associated Coleoptera. The wood was identified according to their anatomy. Diversity was estimated using the Simpson index, while abundance was estimated using a Kruskal-Wallis test; the association of Coleoptera with wood species and decay was assessed using canonical correspondence analysis. Decay wood stage I is the most abundant (51%), followed by stage III (21%). We collected 93 Coleoptera belonging to 14 families, 41 genera, and 44 species. The family Cerambycidae was the most abundant, with 29% of individuals, followed by Tenebrionidae with 27% and Carabidae with 13%. We recognized six feeding guilds. The greatest diversity of Coleoptera was recorded in decaying Acacia farnesiana and Bursera linanoe. Kruskal-Wallis analysis indicated that the abundance of Coleoptera varied according to the species and stage of decay of the wood. The canonical analysis showed that the species and stage of decay of wood determined the composition and community structure of Coleoptera. PMID:26911160

  10. Tanyproctini (Coleoptera: Scarabaeidae: Melolonthinae) of Socotra Island

    Czech Academy of Sciences Publication Activity Database

    Král, D.; Sehnal, R.; Bezděk, Aleš

    2012-01-01

    Roč. 52, suppl. 2 (2012), s. 153-182. ISSN 0374-1036 Grant ostatní: Ministry of Education, Youth and Sports (CZ) LA10036/MSMT Institutional support: RVO:60077344 Keywords : Coleoptera * Scarabaeidae * Melolonthinae Subject RIV: EG - Zoology Impact factor: 0.963, year: 2012 http://www.aemnp.eu/PDF/52_s2/52_S2_153.pdf

  11. NON PREFERENTIAL OVIPOSITION OF Zabrotes subfasciatus IN COMMON BEANS (Phaseolus vulgaris L.) APPLIED WITH SOME VEGETAL PRODUCTS NÃO-PREFERÊNCIA PARA OVOPOSIÇÃO DE Zabrotes subfasciatus (Boheman, 1833) EM FEIJÃO TRATADO COM DIFERENTES PRODUTOS DE ORIGEM VEGETAL

    OpenAIRE

    Corival Cândido da Silva; Elisane Galbe de Carvalho Costa; Heloína Teresinha Faleiro Ramos; Roberto de Toledo Guimarães; Antônio Henrique Garcia

    2007-01-01

    It was studied the non-preferential oviposition of Zabrotes subfasciatus (Boheman, 1833) in common beans (P. vulgaris L.) cv. carioca applied with some vege...

  12. ON THE TAXONOMY AND NOMENCLATURE OF SOME MECININI (COLEOPTERA, CURCULIONIDAE

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    Roberto Caldara

    2008-04-01

    Full Text Available In accordance with the Code, ten actions are taken to preserve nomenclatural stability of names of taxa currently belonging to Mecinini. Following the provisions of ICZN Article 23.9.1, Cleopomiarus graminis (Gyllenhal, 1813 (formerly Rhynchaenus is made a nomen protectum and Curculio ellipticus Herbst, 1795 is made a nomen oblitum; Rhinusa antirrhini (Paykull, 1800 (formerly Curculio is made a nomen protectum and Curculio noctis herbst, 1795 is made a nomen oblitum; having met the conditions of ICZN article 75.3 the neotypes of the following taxa are designated: Curculio antirrhini Paykull, 1800, Curculio cinctus Rossi, 1790, Curculio curvirostris Rossi, 1790, Curculio linariae Panzer, 1792, Cionus thapsicola Germar, 1821, Mecinus collaris Germar, 1821. Lectotypes of Curculio ellipticus Herbst, 1795, Gymnetron eversmanni Rosenschöld, 1838, Mecinus barbarus Gyllenhal, 1838, and Mecinus longiusculus Boheman, 1845 are also designated. Rhinusa linariae (Panzer, 1792 (formerly Curculio remains the valid name of the taxon since Curculio curvirostris Rossi, 1790 (non Fabricius, 1781 nec Herbst, 1784 is unavailable; Mecinus collaris Germar, 1821 remains the valid name of the taxon since Curculio cinctus Rossi, 1790 (non Drury, 1782 nec Geoffroy, 1785 is unavailable. The following new synonymies are proposed: Mecinus barbarus Gyllenhal, 1838 = Mecinus longiusculus Boheman, 1845 n. syn., = Mecinus teretiusculus Boheman, 1845 n. syn., = Mecinus filiformis Aubé, 1850 n. syn.; Rhinusa florum (Rübsaamen, 1895 = Gymnetron smreczynskii Fremuth, 1972 n. syn.; Rhinusa tetra (Fabricius, 1792 = Cionus thapsicola Germar, 1821 n. syn. Rhinusa eversmanni (Rosenschöld, 1838 is the name proposed for Rhinusa thapsicola sensu auctorum (non Germar, 1821.

  13. A review of the Palaearctic species of Larinus Dejean (Coleoptera: Curculionidae in C. J. Schoenherr collection: nomenclature and lectotype designations

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    Levent Gültekin

    2015-06-01

    Full Text Available The syntypes of 41 species of Larinus Dejean described by L. Gyllenhal and C. H. Boheman, housed at the C. J. Schoenherr collection in the Swedish Museum of Natural History, Stockholm and the Museum of Evolution, Uppsala University, are examined. Lectotypes are designated, a nomenclatural review performed, many previous synonyms confirmed and three new synonyms proposed: Larinus planus (Fabricius, 1792 (= Larinus rusticanus Gyllenhal, 1835 syn. nov.; Larinus carlinae (Olivier, 1807 (= Larinus sulphurifer Boheman, 1843 syn. nov.; Larinus minutus Gyllenhal, 1835 (= Larinus puncticollis Capiomont, 1874 syn. nov.. Colour images for 15 lectotypes are presented.

  14. Effectiveness of entomopathogenic nematode Steinernema feltiae in agar gel formulations against larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae)

    Czech Academy of Sciences Publication Activity Database

    Hussein, Hany; Adel, M. M.; Gelbič, Ivan

    2012-01-01

    Roč. 7, č. 1 (2012), s. 77-82. ISSN 1895-104X Institutional research plan: CEZ:AV0Z50070508 Keywords : anti-desiccant * foliar application * drought Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection Impact factor: 0.818, year: 2012

  15. Effects of photoperiod and temperature on reproductive diapause in Ophraella communa (Coleoptera:Chrysomelidae), a potential biocontrol agent against Ambrosia artemisiifolia

    Institute of Scientific and Technical Information of China (English)

    Dao-Hong Zhu; Jing Zhu; Zhao-Pu Peng; Fang-Hao Wan

    2012-01-01

    To investigate the seasonal adaptation strategies of Ophraella communa to new habitats,the effects and regulation mechanisms of photoperiod and temperature on the reproductive diapause in a population collected from Changsha,Hunan were examined.Adults showed obvious reproductive diapause,which was regulated by photoperiod and temperature.At 30℃,there was no adult diapause occurring under either long-day or short-day conditions; at 25℃ the pre-oviposition period was short and fecundity was high in adult females under L:D 16:8 h,whereas under L:D 12:12 h,a few females entered reproductive diapause; at 20℃ under short-day conditions,all female adults entered diapause.The pre-oviposition period was significantly prolonged when the pupae and adults were transferred from long-days to short-days,but the day length influence was not obvious when they were transferred only in the adult stage.However,the fecundity dropped greatly no matter whether the photoperiod shifted to short-days only in the adult stage or whether the shift occurred in both the pupal and adult stage.The fecundity was extremely low when photoperiod shifted from long-days to short-days in both pupal and adult stages.This was an indication that the pupal and adult stages were the photoperiod-sensitive stage for adult reproductive diapause.This was especially true for the photoperiod in the pupal stage,which has a distinctly significant regulative effect on reproductive diapause.Additionally,this article also addresses the reason for different photoperiodic response patterns in reproductive diapause induction between the Changsha strain and the Tsukuba strain (Japan) of O.communa.

  16. Descriptions of eight new species of Phaelota (Coleoptera: Chrysomelidae) with a new generic synonymy and a key to species of Indian subcontinent

    Science.gov (United States)

    Six new species of Phaelota Jacoby from India viz. P. assamensis, P. kottigehara, P. maculipennis, P. mauliki, P. saluki, and P. viridipennis and two new species from Sri Lanka viz. P. ogloblini and P. schereri are described and illustrated. Thrylaea Jacoby is treated as a new junior synonym of Phae...

  17. Taxonomic changes in the genus Diabrotica Chevrolat (Coleoptera: Chrysomelidae: Galerucinae): results of a synopsis of North and Central America Diabrotica species.

    Science.gov (United States)

    Derunkov, A; Konstantinov, A

    2013-01-01

    The following new synonyms in Diabrotica Chevrolat 1836 are proposed: D.flaviventris Jacoby 1887 and D. tibialis Jacoby 1887 are synonyms of D. adelpha Harold 1875; D. peckii Bowditch 1911 is a synonym of D. bioculata Bowditch 1911; D. nummularis Harold 1877 is a synonym of D. circulata Harold 1875; D. linensis Bechyné 1956 is a synonym of D. trifurcata Jacoby 1887; D. brunneosignata Jacoby 1887 is a synonym of D. sinuata Olivier 1790; D. duplicata Jacoby 1887 is a synonym of D. viridifasciata Jacoby 1887. Diabrotica cyaneomaculata Jacoby 1887 does not share the synapomorphies of Diabrotica and is treated as incertae sedis. Diabrotica tripunctata (Fabricius) is removed from synonymy with D. sinuata Olivier and is considered to be a valid species. The original combination is restored for Diabroticafasciata Kirsch, the species being transferred from Paranapiacaba Bechyné back to Diabrotica. It was found that the type series of D. godmani Jacoby contains seven different taxa: one is D. godmani itself; one is D. championi Jacoby; one is D. quadricollis Jacoby; three are unidentified Diabrotica species, each different from the others; and one is not a Diabrotica. The type series of D. viridicollis Jacoby contains four different taxa, D. viridicollis Jacoby itself and three different unidentified Diabrotica species. PMID:26473221

  18. Buttercup squash provides a marketable alternative to blue hubbard as a trap crop for control of striped cucumber beetles (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Cavanagh, Andrew F; Adler, Lynn S; Hazzard, Ruth V

    2010-12-01

    Winter squash is a vital agricultural commodity worldwide. In the Northeastern United States, the primary insect pest is the striped cucumber beetle, Acalymma vittatum F. Using a Blue Hubbard squash (Cucurbita maxima Duchesne) perimeter trap crop system can reduce insecticide use by >90% in butternut squash (C. moschata Poir), the primary winter squash grown in this region. Despite the savings in insecticide costs, growers may be reluctant to give up field space for a perimeter crop of Blue Hubbard squash, which comprises only 5% of the winter squash market in New England as compared with 19% for buttercup squash. Finding a more marketable trap crop would lower the barrier for adoption of this system. We tested eight varieties of three species of cucurbits for attractiveness to beetles relative to Blue Hubbard and butternut squash, and chose buttercup squash as the most promising replacement. We compared the effect of a buttercup border, Blue Hubbard border, or control (no border) on beetle numbers, herbivory, insecticide use, pollination, and pollen limitation in the main crop. We found that buttercup squash performed equally well as Blue Hubbard as a trap crop, with 97% reduction in total insecticide use compared with control fields. Honey bees (Apis mellifera L.) and squash bees (Peponapis pruinosa Say) were the predominant pollinators, and border treatments did not affect visitation. Hand pollination did not increase reproduction or yield, indicating that natural pollination was sufficient for full yield. This study confirms the effectiveness of perimeter trap crop systems and offers growers a more marketable trap crop for managing cucumber beetle damage. PMID:22182562

  19. Nova espécie de Zischkaita Bechyné e notas taxonômicas em Galerucini (Coleoptera, Chrysomelidae, Galerucinae

    Directory of Open Access Journals (Sweden)

    Moura Luciano de A.

    2003-01-01

    Full Text Available A new species of Zischkaita Bechyné, 1956, Z. serrana sp. nov. are described from São Francisco de Paula and Cambará do Sul, Rio Grande do Sul State, Brazil. Two new combinations are proposed: Zischkaita bucki (Bechyné & Bechyné, 1962, comb. nov., removed from Isotes and Cochabamba volxemi (Baly, 1889, comb. nov., transferred from Paranapiacaba.

  20. An analysis of the fate of eggs of Gratiana spadicea (Klug, 1829 (Coleoptera: Chrysomelidae: Cassidinae in relation to the position in the ootheca

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    Miriam Becker

    1987-01-01

    Full Text Available Randomly sampled oothecae of a natural population of Gratiana spadicea from the outskirts of Porto Alegre, RS, were dissected. The overlapping of the egg membranes makes possible to enumerate the eggs in a batch and therefore to relate mortality to position in the ootheca. The ootheca of this cassid provides a spatial refuge for some of its eggs. Successful eggs of G. spadicea amounted to less than one fifth of the total in each batch. Parasitoids and predators were responsible for a high mortality of eggs whatever the size of the ootheca. The main cause of mortality was the eulophid wasp Emersonella ooecia De Santis, 1983. A large proportion of eggs were sucked dry by the mirid Tupiocoris cincticornis (Stal, 1860. The sucked eggs could have contained either the embryo of G. spadicea or the parasitoid in the pre-emergence stages.

  1. New species of Diabrotica Chevrolat (Coleoptera: Chrysomelidae: Galerucinae and a key to Diabrotica and related genera: results of a synopsis of North and Central American Diabrotica species

    Directory of Open Access Journals (Sweden)

    Alexander Derunkov

    2015-02-01

    Full Text Available The following 18 new species of Diabrotica are described and illustrated as a result of the synopsis of North and Central American species: D. barclayi sp. nov., Guatemala; D. caveyi sp. nov., Costa Rica; D. costaricensis sp. nov., Costa Rica; D. dmitryogloblini sp. nov., Mexico; D. duckworthorum sp. nov., Honduras; D. hartjei sp. nov., Panama; D. josephbalyi sp. nov., Costa Rica; D. lawrencei sp. nov., Mexico; D. mantillerii sp. nov., Panama; D. martinjacobyi sp. nov., Honduras; D. mitteri sp. nov., Panama; D. perkinsi sp. nov., Guatemala; D. redfordae sp. nov., Costa Rica; D. reysmithi sp. nov., Costa Rica; D. salvadorensis sp. nov., El Salvador; D. sel sp. nov., Panama; D. spangleri sp. nov., Costa Rica; D. waltersi sp. nov., Panama. In addition, a key to separate Diabrotica from related genera is presented.

  2. Anatomical features of leaves of three cultivars of winter wheat (Triticum aestivum L. and settling the plants by cereal leaf beetles, Oulema spp. (Coleoptera, Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Elżbieta Weryszko-Chmielewska

    2013-12-01

    Full Text Available Investigations of flag leaves anatomy of three winter wheat cultivars: Almari, Gama and Weneda were carried out as it was state that there are great differences in the intensity of cereal leaf beetle feeding on the leaves. In order to determine the features conditioning the differentiated resistance of these cultivars following parameters were measured: the thickness of leaf blade, the length of trichomes and their density in the adaxial epidermis, the number of silicon cells in 1 mm2 epidermis and the thickness of the external cell walls of epidermis. The observations of cross section of the leaves were made in a light microscope and that of surface of the adaxial epidermis in a scanning electron microscope. In this study it was shown that Gama cv. distinguishes of the shortest trichomes with poor density, the lowest number of the silicon cells in 1 mm2 and epidermis cells with the thinest walls. This features indicate a poor resistance of Gama cv. against feeding of the pests and give reasons for the presence a much higher number of the cereal leaf beetle larvae (about 100% than at the extant two cultivars. Dependence between the thickness of leaf blades and the number of larvae of the infesting pests has not been stated.

  3. The evolutionary history of the genus Timarcha (Coleoptera, Chrysomelidae) inferred from mitochondrial COII gene and partial 16S rDNA sequences.

    Science.gov (United States)

    Gómez-Zurita, J; Juan, C; Petitpierre, E

    2000-02-01

    The apterous genus Timarcha consists of three subgenera and more than 100 species in its Palearctic distribution, with specialized feeding on few plant families. Fifty-four sequences sampled from 31 taxa of the genus plus three outgroup leaf beetles were studied for their complete cytochrome oxidase II (COII) and a fragment of 16S rDNA mitochondrial genes, representing a total of about 1200 bp. Phylogenetic analyses using maximum-parsimony and distance methods for each gene separately and for the combined data set gave compatible topologies. The subgenus Metallotimarcha consistently appears in a basal position and is well differentiated from the remaining Timarcha, but no clear monophyletic grouping of Timarchostoma and Timarcha s. str. subgenera can be deduced from our analysis. Calibration of the molecular clock has been done using the opening of the Gibraltar Strait after the Messinian salinity crisis (about 5.5 MYA) as the biogeographic event causing disjunction of two particular taxa. Accordingly, the COII evolutionary rate has been estimated to be of 0.76 x 10(-8) substitution/site/year in Timarcha. Relation between phylogeny and host-plant use indicates widening of trophic regime as a derived character in Timarcha. PMID:10679162

  4. Biochemical characterisation of α-glucosidase and β-glucosidase in the alimentary canal of larval Leptinotarsa decemlineata SAY, 1824 (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Kazzazi Majid

    2014-12-01

    Full Text Available Host plant resistance is an environmentally safe method used for reducing a pest population. Basically, when developing resistant cultivars one needs to study the biochemical characteristics of the digestive enzymes in the insect’s midgut. In this study, the activities of α- and β-glucosidase were determined from Leptinotarsa decemlineata midgut using p-nitrophenyl-α-Dglucopyranoside and p-nitrophenyl-β-D-glucopyranoside as substrates respectively. The results showed that the specific activity of α- and β-glucosidase from 4th instar larvae midguts of L. decemlineata were 5.14 and 5.48 Umg-1 protein respectively. The activity of α-glucosidase was optimal at pH 4, whereas the maximum activity of β-glucosidase in the midgut of L. decemlineata occurred at pH 4-5.5. Both enzymes were stable at pH 3-8 over an incubation time of 8 hours. The respective activities of α- and β-glucosidase were at their highest at 45 °C and 50 °C, but they were not stable at 50 °C during an incubation time of 8 days. Furthermore, our data showed that MgCl2, Tris and urea have a moderate but SDS a severe inhibitory effect on enzyme activity. Biochemical characterisation revealed one and three bands of α- and β-glucosidase activities in the midgut of L. decemlineata respectively.

  5. Effect of temperature on reproduction and embryonic development of the cabbage stem flea beetle, Psylliodes chrysocephala L., (Coleoptera: Chrysomelidae)

    DEFF Research Database (Denmark)

    Mathiasen, Helle; Sørensen, Helle; Bligaard, J.;

    2015-01-01

    °C, respectively. A linear model of egg development at 8–20°C estimated the lower developmental threshold to be 5.1°C and the thermal constant for development 184.9 degree-days. The percentage of eggs hatching was significantly lower at 4°C than at all other temperatures tested. The estimated mean...... parameters of reproduction, egg development and viability at five constant temperatures. Significant temperature effects were found on the pre-oviposition period, total number of eggs laid, daily oviposition rate, female longevity, egg-development rate and viability. The mean length of the pre...

  6. A new species of Longitarsus Latreille, 1829 (Coleoptera, Chrysomelidae, Galerucinae pupating inside stem aerenchyma of the hydrophyte host from the Oriental Region

    Directory of Open Access Journals (Sweden)

    Kaniyarikkal Prathapan

    2011-03-01

    Full Text Available A new species of subaquatic Longitarsus pupating inside the stem aerenchyma of its hydrophyte host plant is described. Eggs are laid on tender leaves and buds and the larvae are open feeders. This is the first report of an Oriental flea beetle pupating inside the stem of its hydrophyte host. A key to the species of southern Indian Longitarsus is provided.

  7. Western Corn Rootworm (Coleoptera: Chrysomelidae) Larval Movement in eCry3.1Ab+mCry3A Seed Blend Scenarios.

    Science.gov (United States)

    Zukoff, Sarah N; Zukoff, Anthony L; Geisert, Ryan W; Hibbard, Bruce E

    2016-08-01

    Corn fields planted with plant-incorporated Bacillus thuringiensis (Bt) proteins must have a portion of the field planted with non-Bt, isoline, plants that serve as a refuge for susceptible insects. In the Corn Belt, refuge seeds are now blended in the bag with Bt seeds for corn hybrids containing two or more toxins targeted toward the western corn rootworm, Diabrotica virgifera virgifera LeConte. Syngenta's corn hybrid, Agrisure Duracade, containing the eCry3.1Ab (event 5307) and mCry3a (event MIR604) rootworm-targeted toxins were registered as a seed blend in 2014. Western corn rootworm larval movement between the refuge plants and the Duracade plants was assessed to determine western corn rootworm survival and amount of root damage on these plants when planted in all possible seed blend scenarios. In this study, western corn rootworm larvae moved between isoline and Bt plants and adult survival was greater on Bt plants if movement from a neighboring infested isoline plant had occurred. However, root damage to these Bt plants did not reach economic levels. The low numbers of western corn rootworm larvae that did move from an infested Bt plant to an isoline plant could potentially select for resistance if they survived to adulthood. PMID:27190042

  8. Field testing Diorhabda elongata (Coleoptera:Chrysomelidae) from Crete, Greece to assess potential impact to non-target native California plants in the genus Frankenia

    Science.gov (United States)

    When laboratory host specificity tests on weed biological control agents produce ambiguous results or are suspected of producing false positives, field cage or open field tests can be utilized in an attempt to determine the true ecological host range of the agent. The leaf beetle Diorhabda elongata ...

  9. Descrição da larva de 3º ínstar e redescrição do adulto de Phaedon confinis Klug (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    2015-08-01

    Abstract. In view of the potential of Phaedon confinis Klug as an biological control agent of the Senecio brasiliensis Less and the lack of taxonomic knowledge of the species Phaedon recorded for Brazil, this paper aimed to describe the 3rd larval instar, redescribe the adult, as well as add new characters and illustrations to acknowledgment of the specie. The P. confinis larvae are eruciform, subcylindrical, elongated and slightly convex; dorsal surface dark brown and ventral surface with yellowish lighter regions; four setae in the front; six stemmata arranged in three groups; brown labrum, with four pairs of setae, pronotal plate with ten setae; pleural region of prothorax with tubercle T; meso- and metathorax with tubercles Dai and Dae close, but not fused, and tubercles Es-Ss fused between themselves by midline; simple tarsal claws; abdomen with tubercle Dae absent and Dai present, little pronounced; abdominal tubercles with two setae in Dpi and Dpe. Adult has oval body and dorsally convex; pronotum and elytral glabrous; predominant colour blue metallic violet; lateral margins of pronotum rounded, yellowish brown with central macula metallic blue, elytral striae with deep punctuation and arranged in eight longitudinal rows; yellowish brown regions on the head, prothorax and legs; head with two small oval black maculae, posterior to the eyes in vertex region.

  10. Open-field host specificity test of Gratiana boliviana (Coleoptera: Chrysomelidae), a biological control agent of tropical soda apple (Solanaceae) in the United States

    International Nuclear Information System (INIS)

    An open-field experiment was conducted to assess the suitability of the South American leaf feeding beetle Gratiana boliviana Spaeth for biological control of Solanum viarum Dunal in the USA. An open-field test with eggplant, Solanum melongena L., was conducted on the campus of the University of Buenos Aires, Argentina, and a S. viarum control plot was established 40 km from the campus. One hundred adult beetles were released in each plot at the beginning of the experiment during the vegetative stage of the plants, and forty additional beetles were released in the S. melongena plot at the flowering stage. All the plants in each plot were checked twice a week and the number of adults, immatures, and eggs recorded. Results showed almost a complete rejection of eggplant by G. boliviana. No noticeable feeding damage was ever recorded on eggplant. The experiment was ended when the eggplants started to senesce or were severely damaged by whiteflies and spider mites. The results of this open-field experiment corroborate previous quarantine/laboratory host-specificity tests indicating that a host range expansion of G. boliviana to include eggplant is highly unlikely. Gratiana boliviana was approved for field release in May 2003 in the USA. To date, no non-target effects have been observed either on eggplant or native species of Solanum. (author)

  11. Evaluation of Potential Fitness Costs Associated With eCry3.1Ab Resistance in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Geisert, Ryan W; Hibbard, Bruce E

    2016-08-01

    Both an eCry3.1Ab-selected and paired control western corn rootworm, Diabrotica virgifera virgifera LeConte, colony were tested for adult longevity, egg oviposition, egg viability, and larval development in order to evaluate the potential fitness costs associated with eCry3.1Ab resistance. Adult longevity experiments were conducted by pairing virgin males and females together in plastic boxes supplied with food, water, and ovipositional medium and observed for survival time. Eggs were also collected from the ovipositional medium once a week to determine average egg oviposition and egg viability. Larval development time experiments were conducted by infesting seedling assays with 25 neonate larvae and recording larval recovery after several days. Adult longevity, average egg oviposition, and larval development time results indicated a lack of fitness costs associated with eCry3.1Ab resistance in the western corn rootworm. Results of egg viability indicated a fitness advantage for the eCry3.1Ab-selected colony with a significantly higher egg hatch than the control. PMID:27151470

  12. The genetics of two colour forms of Chrysolina aurichalcea (Mannerheim) (Coleoptera: Chrysomelidae) and these gene frequencies around the Utsukushigahara Heights, central Honshu, Japan.

    OpenAIRE

    FUJIYAMA, SHIZUO; ARIMOTO, KINJI; TANABE, MINORU

    1987-01-01

    The inheritance pattern of the two colour forms (cyaneus-form and cupreous-form) of adult Chrysolina aurichalcea (Mannerheim) was examined by the crossing. The inheritance of these colour forms followed Mendel's law, showing the dominance of cyaneus-form over cupreous-form. The sampling of field populations around the Utsukushigahara Heights, central Honshu, Japan, was carried out at 74 sites. The estimation based on these results showed that the gene frequencies of cupreous-form were high (8...

  13. Susceptibility of Northern Corn Rootworm Diabrotica barberi Smith (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins

    Science.gov (United States)

    Susceptibility of the northern corn rootworm (NCR), to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis (Bt) was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Lar...

  14. New DNA markers reveal presence of Aphthona species (Coleoptera: Chrysomelidae) believed to have failed to establish after release into leafy spurge

    Science.gov (United States)

    Six species of Aphthona flea beetles from Europe have been introduced in North America for the purpose of controlling a noxious weed, leafy spurge (Euphorbia esula). In the years following the releases, five of the species have been recorded as being established at various locations. There is no evi...

  15. Preferência alimentar de Phaedon confinis Klug (Coleoptera, Chrysomelidae frente a quatro espécies de Senecio (Asteraceae de interesse pecuário

    Directory of Open Access Journals (Sweden)

    Julianne Milléo

    2014-12-01

    Abstract. One of the main causes of death in cattle is related to poisoning by plants of Senecio spp. (Asteraceae. Thus, alternatives for reduction of these plants in the Southern region of Brazil are necessary to diminish losses in livestock. Studies have demonstrated the chrysomelid Phaedon confinis Klug as a potential biological agent of Senecio brasiliensis Less. Our study aimed to evaluate the feeding preference of larvae and adults of P. confinis by the species S. brasiliensis, Senecio madagascariensis Poiret, Senecio oxyphyllus DC and Senecio selloi DC. Previously, the insects were fed on S. brasiliensis to the experiment at 22 ± 1oC temperature, 60 ± 5% relative humidity and 12h photophase. Results obtained indicate that injury caused by P. confinis depended on the species of Senecio offered to the insect, with higher consumption of S. brasiliensis. The developmental stage of the insect influences the feeding preference, since neonate larvae demonstrated higher selectivity to the plant host than adults. In addition to S. brasiliensis, adults can be used to control other species of Senecio, especially S. madagascariensis, since there is oviposition on these plants.

  16. New contributions to the molecular systematics and the evolution of host-plant associations in the genus Chrysolina (Coleoptera, Chrysomelidae, Chrysomelinae)

    Science.gov (United States)

    Jurado-Rivera, José A.; Petitpierre, Eduard

    2015-01-01

    Abstract The taxonomic circumscription of the large and diverse leaf beetle genus Chrysolina Motschulsky is not clear, and its discrimination from the closely related genus Oreina Chevrolat has classically been controversial. In addition, the subgeneric arrangement of the species is unstable, and proposals segregating Chrysolina species into new genera have been recently suggested. In this context, the availability of a phylogenetic framework would provide the basis for a stable taxonomic system, but the existing phylogenies are based on few taxa and have low resolution. In the present study we perform a phylogenetic analysis based on mitochondrial (cox1 and rrnL) and nuclear (H3) DNA sequences from a sample of fifty-two Chrysolina species representing almost half of the subgeneric diversity of the group (thirty out of sixty-five subgenera) and most of the morphological, ecological and karyological variation in the genus. In addition, five Oreina species from two subgenera have also been analysed. The resulting phylogeny is used to evaluate some of the most relevant taxonomic hypotheses for Chrysolina, and also to reconstruct its ancestral host plant associations in a Bayesian framework. Our findings support the paraphyly of Chrysolina as currently defined due to the inclusion of Oreina, the monophyly of the Chrysolina (plus Oreina) species including the divergent Chrysolina (Polysticta) vigintimaculata (Clark, 1864), and enable inferences of deep-level evolutionary relationships among the studied subgenera. The plant family Lamiaceae is inferred as the ancestral host of the study group, whose evolution is characterized by continuous host-shifting among pre-existing host plant families. Some Chrysolina clades include mixtures of species with different levels of diet breadth, indicating that niche width has varied through time. PMID:26798320

  17. On the influence of different host plants and of insecticide treatments on the population development of the western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae

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    Fora, Ciprian George

    2014-02-01

    Full Text Available D. virgifera virgifera is classified as a quarantine pest in Germany, therefore the trials, presented in this paper, were performed in the western part of Romania, where the pest is well established since more than ten years. The field tests were carried out in close collaboration with Banat’s University of Agricultural Sciences and Veterinary Medicine, Timişoara. On sites highly infested by D. virgifera virgifera, 1 m² plots in four replications per variable were planted with maize for the chemical treatments and alternative crops (cereals and weeds. Gauze covered hatch cages were used for weekly counts of the emerged adult Diabrotica during the hatch period from mid of June to mid of August. It could be asserted that matured cereals are no host-plants for the western corn rootworm. In four years of trials not even one adult beetle hatched in the related cages. Setaria viridis and Digitaria sanguinalis reduced the number of hatched imagines significantly, nevertheless some individuals survived. Therefore an effective herbicide management against grass weeds in maize is recommended to limit the chance of survival of the pest. Clothianidin and Tefluthrin are effective against D. virgifera virgifera. 20% to 100% efficiency was assessed in the trials, strongly depending on precipitation and soil moisture in time of application. The insecticides decreased the maize root injury caused by larvae of the western corn rootworm significantly.

  18. Laboratory test of the potential for using insecticide-cucurbitacin mixtures for controlling the quarantine pest Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae

    Directory of Open Access Journals (Sweden)

    Buuk, Christoph

    2014-02-01

    Full Text Available Due to the great ecological plasticity and adaptability of the western corn rootworm, Diabrotica virgifera virgifera LeConte, the unilateral use of one control option could result in it becoming less effective within a few years. Therefore, no one option alone is sufficient for resolving the problem with D. virgifera virgifera. In fact all possibilities have to be considered and integrated. One possibility is to control the adult beetles and so minimize egg deposition and reduce the beetle population size below the economic threshold for the following year. By applying mixtures of insecticides with bitter substances from cucurbits, which are strong feeding stimulants for D. virgifera virgifera, it should be possible to reduce insecticide dosages by up to 95%. This would control the beetles and minimize undesired side-effects to the agro-ecosystem and non-target organisms. Therefore, the aim of this study was to determine (i the interactions between cucurbitacins (Invite and five insecticides with different odes of action (indoxacarb, neonicotinoids, organophosphates, pyrethroids and spinosyns, (ii the effects of biological factors (age, gender and pre-contact and (iii the possibility of selection for resistance to bitter agents. In laboratory trials it was shown that the stimulatory effect Invite has on feeding had little or no effect on the efficacy of the five insecticides tested. The improvement in the efficacy after five hours of exposure to Avaunt (indoxacarb and Biscaya (neonicotinoid disappeared after 24 and 48 h, and is attributed to the slow initial effects of Avaunt and the recovery of beetles exposed to Biscaya, respectively. Although the LC-values of Biscaya and Avaunt were significantly greater than the corresponding values for mixtures with Invite, it was not possible to reduce the dosages of these active substances by up to 90%. There was no improvement in the efficacy after 48 h of exposure to any of the other insecticides analyzed. The assumption that contact (e. g. pyrethroides and gas phase insecticides (e. g. organophosphate are generally less suitable for mixing with Invite was only partly supported by our results. The lack of improvement in the efficacy of Spinosad when mixed with Invite is especially puzzling. Neonicotinoids and indoxacarb are suitable for mixing with Invite, especially in terms of delaying the selection for resistance. Carbamates, e. g. carbaryl (not analyzed in this study, were successfully applied in mixtures with cucurbitacins. The results indicate that biological factors such as gender, age and pre-contact have a strong effect on the attractiveness of Invite. In the experiments, the strength of the response of young beetles to the bitter agents was greater than that of old beetles and that of females less intense than that of males, and pre-contact markedly reduced the stimulatory effect for both sexes. These results and the findings of trials using D. virgifera virgifera caught in Austrian maize fields before and after applications of insecticide-Invite mixtures indicate that the attractiveness of cucurbitacins varies and is subject to selection. If this control strategy is applied extensively then it is likely that this beetle will develop resistance to the bitter agents. Thus, it is important to monitor the resistance of the beetles to these substances.

  19. The interactions of Tropical soda apple mosaic tobamovirus and Gratiana boliviana (Coleoptera: Chrysomelidae), an introduced biological control agent of tropical soda apple (Solanum viarum)

    Science.gov (United States)

    Tropical soda apple (Solanum viarum Dunal (Solanaceae) (TSA) is a South American invasive plant of rangelands, pastures and natural areas in Florida. A chrysomelid beetle from South America, Gratiana boliviana Spaeth, has been released at >300 locations in Florida for biological control of TSA sinc...

  20. Impact of αAI-1 expressed in genetically modified cowpea on Zabrotes subfasciatus (Coleoptera: Chrysomelidae) and its parasitoid, Dinarmus basalis (Hymenoptera: Pteromalidae).

    Science.gov (United States)

    Lüthi, Christoph; Alvarez-Alfageme, Fernando; Romeis, Jörg

    2013-01-01

    Genetically modified (GM) cowpea seeds expressing αAI-1, an α-amylase inhibitor from the common bean, have been shown to be immune against several bruchid species. Effective control of such pests by growing GM cowpea could promote the spread of bruchid species that are αAI-1 tolerant. Consequently, the sustainability of bruchid pest control could be increased by combining GM seeds and hymenopteran parasitoids. However, there are concerns that αAI-1 could interfere with the biological control provided by parasitoids. Here, we assessed the impact of GM cowpea seeds expressing αAI-1 on the αAI-1-tolerant bruchid Zabrotes subfasciatus and its parasitoid Dinarmus basalis. αAI-1 in cowpea seeds did not increase resistance to Z. subfasciatus or affect the mortality rate of Z. subfasciatus larvae. Parasitism of Z. subfasciatus by D. basalis and fitness of D. basalis offspring were not affected by the presence of αAI-1. Thus, αAI-1-expressing cowpeas and parasitoids should be compatible for the control of bruchid pests. PMID:23840776

  1. Impact of αAI-1 Expressed in Genetically Modified Cowpea on Zabrotes subfasciatus (Coleoptera: Chrysomelidae) and Its Parasitoid, Dinarmus basalis (Hymenoptera: Pteromalidae)

    OpenAIRE

    Lüthi, Christoph; Álvarez-Alfageme, Fernando; Romeis, Jörg

    2013-01-01

    Genetically modified (GM) cowpea seeds expressing αAI-1, an α-amylase inhibitor from the common bean, have been shown to be immune against several bruchid species. Effective control of such pests by growing GM cowpea could promote the spread of bruchid species that are αAI-1 tolerant. Consequently, the sustainability of bruchid pest control could be increased by combining GM seeds and hymenopteran parasitoids. However, there are concerns that αAI-1 could interfere with the biological control ...

  2. Susceptibility of northern corn rootworm Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins

    Science.gov (United States)

    Susceptibility of the northern corn rootworm (NCR), to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis (Bt) was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Lar...

  3. Assessing the phylogenetic usefulness of a previously neglected morphological structure through elliptic Fourier analyses: a case study in Bruchus seed-beetles (Coleoptera: Chrysomelidae: Bruchinae)

    OpenAIRE

    Kergoat, Gaël J.; Alvarez, Nadir

    2008-01-01

    We address the systematics of Bruchus seed-beetles through the use of a geometric morphometric outline approach, namely elliptic Fourier analysis. We found that a previously neglected genitalic structure, the ventral plate, provides new evidence in the discussion of taxonomic issues raised by recent molecular studies. Three methods of hierarchical clustering allow investigation of the phylogenetic relationships of the key species that cause the paraphyly of two species groups in recent molecu...

  4. Host preference between saltcedar (Tamarix spp.) and native non-target Frankenia spp. within the Diorhabda elongata species complex (Coleoptera: Chrysomelidae)

    Science.gov (United States)

    Since its release in 2001 for the biological control of saltcedar (Tamarix spp., Tamaricaceae), the leaf beetle Diorhabda elongata (Brullé) from China, has become successfully established in many locations in the western United States. However, it failed to establish in the southern and western por...

  5. The genus Paraplotes Laboissière, 1933 in Taiwan, a speciose group with brachelytrous females (Coleoptera: Chrysomelidae: Galerucinae).

    Science.gov (United States)

    Lee, Chi-Feng

    2015-01-01

    Taiwanese members of the genus Paraplotes comprise a group of species that are not distinguishable based on external morphology but are diagnosed using distributions, aedeagal, and gonocoxal morphologies. Females of all species are brachelytrous. The group includes one previously described species, Paraplotes taiwana Chûjô, 1963, and nine new species, P. cheni sp. nov., P. jengi sp. nov., P. meihuai sp. nov., P. tahsiangi sp. nov., P. tatakaensis sp. nov., P. tsoui sp. nov., P. tsuenensis sp. nov., P. yaoi sp. nov., and P. yuae sp. nov. Diagnostic characters and hind wings of both sexes are illustrated. Models of speciation for the high diversity of Paraplotes in Taiwan are discussed. A novel hypothesis for brachelytrous leaf beetles occurring in tropical forest habitats (selva) is proposed.  PMID:25660781

  6. Carbon isotope ratios document that the elytra of western corn rootworm (Coleoptera: Chrysomelidae) reflects adult versus larval feeding and later instar larvae prefer Bt corn to alternate hosts.

    Science.gov (United States)

    Hiltpold, Ivan; Adamczyk, John J; Higdon, Matthew L; Clark, Thomas L; Ellersieck, Mark R; Hibbard, Bruce E

    2014-06-01

    In much of the Corn Belt and parts of Europe, the western corn rootworm, Diabrotica virgifera virgifera LeConte, is the most important insect pest of maize. The need for additional basic knowledge of this pest has been highlighted while developing resistance management plans for insecticidal genetically modified crops. This study evaluated the possibility of tracking feeding habits of western corn rootworm larvae using stable carbon isotope signatures. Plants accumulate different ratios of (13)C:(12)C isotopes, usually expressed as δ(13)C, according to whether they use the C3 or C4 photosynthetic pathway. Herbivore biomass is expected to reflect the δ(13)C of the food they eat. For the current experiment, western corn rootworm larvae were grown on different species of plants exhibiting different δ(13)C values. The δ(13)C values were then measured in elytra of emerged beetles. When beetles were unfed, biomass reflected larval feeding. When beetles were fed for 31 d postemergence, δ(13)C values of elytra almost exclusively reflected adult feeding. These results suggest the use of caution in the interpretation of δ(13)C data aiming to document larval diet history when adult feeding history is unknown. The technique was also used to evaluate western corn rootworm larval choice between alternate hosts and maize with and without genetically modified (Bt) traits aimed at their control. Propensity for feeding on alternate hosts versus maize was biased toward feeding on maize regardless whether the maize had Bt or not, suggesting western corn rootworm larvae were not repelled by Bt. These data will be helpful for regulators in interpreting western corn rootworm feeding data on Bt maize. PMID:24874160

  7. New contributions to the molecular systematics and the evolution of host-plant associations in the genus Chrysolina (Coleoptera, Chrysomelidae, Chrysomelinae).

    Science.gov (United States)

    Jurado-Rivera, José A; Petitpierre, Eduard

    2015-01-01

    The taxonomic circumscription of the large and diverse leaf beetle genus Chrysolina Motschulsky is not clear, and its discrimination from the closely related genus Oreina Chevrolat has classically been controversial. In addition, the subgeneric arrangement of the species is unstable, and proposals segregating Chrysolina species into new genera have been recently suggested. In this context, the availability of a phylogenetic framework would provide the basis for a stable taxonomic system, but the existing phylogenies are based on few taxa and have low resolution. In the present study we perform a phylogenetic analysis based on mitochondrial (cox1 and rrnL) and nuclear (H3) DNA sequences from a sample of fifty-two Chrysolina species representing almost half of the subgeneric diversity of the group (thirty out of sixty-five subgenera) and most of the morphological, ecological and karyological variation in the genus. In addition, five Oreina species from two subgenera have also been analysed. The resulting phylogeny is used to evaluate some of the most relevant taxonomic hypotheses for Chrysolina, and also to reconstruct its ancestral host plant associations in a Bayesian framework. Our findings support the paraphyly of Chrysolina as currently defined due to the inclusion of Oreina, the monophyly of the Chrysolina (plus Oreina) species including the divergent Chrysolina (Polysticta) vigintimaculata (Clark, 1864), and enable inferences of deep-level evolutionary relationships among the studied subgenera. The plant family Lamiaceae is inferred as the ancestral host of the study group, whose evolution is characterized by continuous host-shifting among pre-existing host plant families. Some Chrysolina clades include mixtures of species with different levels of diet breadth, indicating that niche width has varied through time. PMID:26798320

  8. Molecular markers detect cryptic predation on coffee berry borer (Coleoptera: Curculionidae) by silvanid and laemophloeid flat bark beetles (Coleoptera: Silvanidae, Laemophloeidae) in coffee beans

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei(Coleoptera: Curculionidae)(Ferrari), is a serious pest of coffee worldwide and has been recently introduced in Hawai’i, first detected in the state in 2010. Adult silvanid flat bark beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and adult laemoph...

  9. Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera)

    NARCIS (Netherlands)

    Alexander, K.

    2003-01-01

    Changing distributions of Cantharidae and Buprestidae within Great Britain (Coleoptera) Data are presented on the distribution of selected species from two coleopteran families chosen to represent a random slice of the British fauna. The species have been chosen as exhibiting extremes of range chang

  10. A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

    Science.gov (United States)

    One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

  11. The Peritelini (Coleoptera: Curculionidae, Entiminae of the Vitale collection

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    Cosimo Baviera

    2016-01-01

    Full Text Available The collection of Peritelini (Coleoptera Curculionidae Entiminae currently stored in the Vitale collection of Messina University is an element of great importance for studies of taxonomy and biogeography of these rarely collected weevils. All species are commented in relation to the contributions to this taxonomic group, published on several occasions by the authors.

  12. Molecular and Insecticidal Characterization of a Cry1I Protein Toxic to Insects of the Families Noctuidae, Tortricidae, Plutellidae, and Chrysomelidae

    OpenAIRE

    Ruiz de Escudero, Iñigo; Estela, Anna; Porcar, Manuel; Martínez, Clara; Oguiza, José A.; Escriche, Baltasar; Ferré, Juan; Caballero, Primitivo

    2006-01-01

    The most notable characteristic of Bacillus thuringiensis is its ability to produce insecticidal proteins. More than 300 different proteins have been described with specific activity against insect species. We report the molecular and insecticidal characterization of a novel cry gene encoding a protein of the Cry1I group with toxic activity towards insects of the families Noctuidae, Tortricidae, Plutellidae, and Chrysomelidae. PCR analysis detected a DNA sequence with an open reading frame of...

  13. Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

    Science.gov (United States)

    Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

  14. Het voorkomen van de glanskevers van het genus Caprophilus in Nederland (Coleoptera: Nitidulidae)

    NARCIS (Netherlands)

    Oude, de J.

    2005-01-01

    The occurrence of nitidulid beetles of the genus Carpophilus in the Netherlands (Coleoptera: Nitidulidae) Some species of the genus Carpophilus (Coleoptera: Nitidulidae) may act as pests of stored products like cocoabeans, peanuts, dried fruits like figs and dates, imported from tropical or subtropi

  15. Cholini (Coleoptera: Curculionidae: Molytinae housed in the Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil Cholini (Coleoptera: Curculionidae, Molytinae depositados na Coleção de Invertebrados do Instituto Nacional de Pesquisas da Amazônia

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    Márcio Luís Leitão Barbosa

    2011-01-01

    Full Text Available In Brazilian Amazonia, Cholini (Coleoptera, Curculionidae, Molytinae is represented by 53 species distributed in seven genera: Ameris Dejean, 1821; Cholus Germar, 1824; Homalinotus Sahlberg, 1823; Lobaspis Chevrolat, 1881; Odontoderes Sahlberg, 1823; Ozopherus Pascoe, 1872 and Rhinastus Schoenherr, 1825. This work documents the species of Cholini housed in the Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil and gives the geographical and biological data associated with them. A total of 186 Cholini specimens were identified as belonging to 14 species (13 from Brazilian Amazonia and five genera (Cholus, Homalinotus, Odontoderes, Ozopherus and Rhinastus. Only 24% of the Cholini species reported from Brazilian Amazonia are actually represented in the INPA collection, underscoring the need for a more systematical collecting based on available biological information. The known geographical distribution was expanded for the following species: Cholus granifer (Chevrolat, 1881 for Brazil; C. pantherinus (Olivier, 1790 for Manaus (Amazonas; Cholus parallelogrammus (Germar, 1824 for Piraquara (Paraná; Homalinotus depressus (Linnaeus, 1758 for lago Janauacá (Amazonas and rio Tocantins (Pará; H. humeralis (Gyllenhal, 1836 for Novo Airão, Coari (Amazonas and Porto Velho (Rondônia; H. nodipennis (Chevrolat, 1878 for Carauari, Lábrea (Amazonas and Ariquemes (Rondônia; H. validus (Olivier, 1790 for rio Araguaia (Brasil, Manaus (Amazonas, rio Tocantins (Pará, Porto Velho and BR 364, Km 130 (Rondônia; Odontoderes carinatus (Guérin-Méneville, 1844 for Manaus (Amazonas; O. spinicollis (Boheman, 1836 for rio Uraricoera (Roraima; and Ozopherus muricatus Pascoe, 1872 for lago Janauacá (Amazonas. Homalinotus humeralis is reported for the first time from "urucuri" palm, Attalea phalerata Mart. ex Spreng.Na Amazônia brasileira, Cholini (Coleoptera, Curculionidae, Molytinae é representada por 53 espécies, distribu

  16. Feeding preference of Diabrotica speciosa (Ger. (Coleoptera: Chrysomelidae by broccoli leaves from natural, organic and conventional farming systems/ Preferência alimentar de Diabrotica speciosa (Ger. (Coleoptera: Chrysomelidae por folhas de brócolos cultivado em sistema natural, orgânico e convencional

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    Pedro Manuel O. J. Neves

    2006-06-01

    Full Text Available Multiple-choice laboratory tests were achieved to compare feeding preference of Diabrotica speciosa (Ger. to leaves of broccoli (Brassica oleraceae L. var. italica from natural, conventional and organic farming systems. Natural farming systems included incorporation of the elephant grass Pennisetum purpureum Schumacher cv. Napier (50 ton/ha, Bokashi compost (1.5 ton/ha and spray of EM 4 (Natural 1, or the incorporation of the Bokashi compost (1.5 ton /ha and spray of EM 4 (Natural 2, and in the conventional, NPK + borax were incorporated in the planting + dressing N and organic compost (1 kg/ plant was incorporated in the organic system. Organic compost was prepared using crop residues of corn (Zea mays L., soybean [Glycine max (L. Mer.], and cattle manure. Leaf discs were collected and placed in cages in multiple-choice tests. Beetles preferred mostly broccoli leaves from conventional farming system than leaves from Natural (1 and 2 and Organic farming systems. Feeding on leaves from Natural 1, Natural 2 and Organic farming system were 68, 67 and 57% of the feeding on leaves from Conventional farming system.Testes de múltipla escola foram realizados para comparar a preferência alimentar de Diabrotica speciosa (Ger. por folhas de brócolos (Brassica oleraceae L. var. italica cultivado em sistema natural, convencional e orgânico. No sistema natural de cultivo houve a incorporação de capim elefante Pennisetum purpureum Schumacher cv. Napier (50 ton/ha, composto Bokashi (1,5 ton/ha e pulverização de EM 4 (Natural 1, ou a incorporação do composto Bokashi (1,5 ton/ha e pulverização do EM 4 (Natural 2, no sistema convencional houve a incorporação do NPK + borax + N em cobertura, e no sistema orgânico incorporouse composto orgânico (1 kg/planta. O composto orgânico foi preparado utilizando-se resíduos de milho (Zea mays L. e soja [Glycine max (L. Mer.] e esterco de gado. Folhas foram retiradas das plantas das quais foram separados discos, e colocados em gaiolas em testes de múltipla escolha. Os insetos preferiram folhas do sistema convencional. A alimentação nas folhas do sistemas Natural 1, Natural 2 e Orgânico foi 68, 67 e 57% daquela registrada nas folhas do sistema convencional de cultivo.

  17. Relação entre tricomas foliares de genótipos de feijoeiro comum, Phaseolus vulgaris L. e resistência a Diabrotica speciosa Germar, 1824 (Coleoptera: Chrysomelidae Relationship between foliar trichomes of common bean genotypes, Phaseolus vulgaris L., and resistance to Diabrotica speciosa Germar, 1824(Coleoptera: Chrysomelidae

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    Maria José Fachini de Oliveira Paron

    2005-08-01

    Full Text Available Considerando que a pubescência foliar pode afetar a preferência alimentar do inseto, com o presente estudo objetivou-se verificar a influência da pilosidade dos folíolos na resistência de genótipos de feijoeiro a D. speciosa. O experimento foi conduzido em delineamento experimental inteiramente casualizado, com seis tratamentos e oito repetições. Os tratamentos foram seis genótipos de feijoeiro: Emgopa Ouro e IAPAR 57, do centro de domesticação Mesoamericano (MA e Jalo Precoce, Goiano Precoce, PR 10595142 e PR 10595146 do centro de domesticação Andino (AN. Cada parcela foi constituída por um vaso com 1,2 Kg de solo e uma planta encerrada em um telado individual. No 25º dia após o plantio, em cada telado foram liberados dez adultos de D. speciosa, após jejum de 24 horas, durante 72 horas. Os danos sofridos foram estimados de acordo com a área consumida em cada genótipo. Os tricomas das folhas dos genótipos foram contados usando microscópio estereoscópico (40x, com uma escala micrométrica sobre 1 cm² de área da superfície abaxial (AB e adaxial (AD, contando-se os tricomas unciformes e aciculares presentes em cinco folhas de cada genótipo. O genótipo IAPAR 57 (MA foi o preferido no teste de alimentação, enquanto os genótipos andinos foram menos consumidos por adultos de D. speciosa. Os genótipos apresentaram variabilidade quanto ao número de tricomas, tanto aciculares quanto unciformes, em ambas as superfícies, abaxial e adaxial. Os genótipos andinos não diferiram quanto ao número de tricomas unciformes na superfície adaxial. O somatório do número de tricomas aciculares e unciformes na superfície abaxial dos genótipos sugerem relação inversa entre esta característica e a área consumida por D. speciosa.Considering that foliar pubescence may affect insect feeding preference on plant genotypes, the purpose of this study was to evaluate the influence of leaflet pubescence on resistance of common bean genotypes to D. speciosa. The experiments were conducted in a completely randomised design, with six treatments and eight replicates. The treatments were six common bean genotypes, Emgopa Ouro and IAPAR 57, from Mesoamerican domestication center (MA, Jalo Precoce, Goiano Precoce, PR 10595142 and PR 10595146 from Andean domestication center (AN. Each replicate consisted of one pot with 1.2 kg soil and a plant placed in a individual cage. The genotypes were submitted to feeding test 25 days after planting, when ten adults of D. speciosa, starved for 24 hours, were released for 72 hours in each cage. Damage was estimated by area of foliage consumed on each genotype. Trichomes on both the abaxial (AB and adaxial (AD leaf surfaces were counted using a stereomicroscope (40 X, on 1 cm² area, with the hooked and straight trichomes being counted on five leaflets/genotype. The MA genotype IAPAR 57 was the most preferred on feeding test, while the AN genotypes were less eaten by Dispeciosa. The genotypes showed variability aceading to density of trichomes on both surfaces. Andean genotypes showed no difference in unciform trichome densities on AD surface. The total number of straight and hooked trichomes on AB leaf surface suggests inverse relation between this characteristic and the area consumed by Diabrotica speciosa.

  18. Susceptibilidade de larvas de Cerotoma arcuata Olivier (Coleoptera: Chrysomelidae a Beauveria bassiana (Bals. Vuillemin, Metarhizium anisopliae (Metsch. Sorokin e Bacillus thuringiensis Berliner Susceptibility of Cerotoma arcuata Olivier (Coleoptera: Chrysomelidae larvae to Beauveria bassiana (Bals. Vuillemin, Metarhizium anisopliae (Metsch. Sorokin and Bacillus thuringiensis Berliner

    Directory of Open Access Journals (Sweden)

    Maria Lucia França Teixeira

    2007-02-01

    Full Text Available Larvas de 2° instar de Cerotoma arcuata foram avaliadas em relação à susceptibilidade aos fungos entomopatogênicos Beauveria bassiana, Metarhizium anisopliae e a bactéria Bacillus thuringiensis com as toxinas Cry3. Os insetos adultos foram mantidos em gaiolas e alimentados com plântulas de feijão (Phaseolus vulgaris L. e as larvas em "gerbox" com cotilédones de plântulas de feijão recém-germinadas. Das oito estirpes de B. bassiana avaliadas, CG 156 e CG 213 causaram 100% de mortalidade das larvas, as duas estirpes de M. anisopliae CG 210 e CG 321 foram patogênicas, eliminando 80 e 100% das larvas de C. arcuata, e, das cinco estirpes de B. thuringiensis testadas, o isolado CG 940 causou 70% de mortalidade das larvas.Second instar larvae of Cerotoma arcuata were evaluated concerning the susceptibility to fungi Beauveria bassiana and Metarhizium anisopliae and Bacillus thuringiensis strains containing Cry3 toxin. Adults of C. arcuata were kept in large cages and fed on bean seedlings and the larvae were reared in ‘gearbox’ feeding on germinated Phaseolus bean cotyledons. Strains CG 156 and CG 213 of B. bassiana killed 100% of the insect larvae and strains CG 210 and CG 321 of M. anisopliae killed 80 and 100% of the insect larvae. Strain CG 940 of B. thuringiensis killed 70% of the insect larvae.

  19. Dimorfismo sexual em Metaxyonycha angusta (Perty, 1832 (Coleoptera: Chrysomelidae - DOI: 10.4025/actascibiolsci.v27i2.1321 Sex determination in Metaxyonycha angusta (Perty, 1832 (Coleoptera: Chrysomelidae - DOI: 10.4025/actascibiolsci.v27i2.1321

    Directory of Open Access Journals (Sweden)

    Norivaldo dos Anjos

    2005-03-01

    Full Text Available O besouro Metaxyonycha angusta é um dos principais desfolhadores de eucalipto, resultando seu ataque em consideráveis perdas na produção de madeira. O objetivo deste trabalho foi identificar caracteres morfológicos que permitam realizar a sexagem em M. angusta. Insetos adultos foram coletados no município de Andrêlandia, Estado de Minas Gerais. A avaliação das características morfológicas externas foi realizada em 50 insetos de cada sexo. Procuraram-se características morfológicas externas para diferir os sexos dos indivíduos dessa espécie, tais como a forma dos uroesternitos e as dimensões corporais. A sexagem de em>M. angusta deve ser realizada examinando-se o formato do 5º uroesternito. Nos machos, a margem posterior apresenta um leve recorte e, nas fêmeas, a margem posterior desse mesmo segmento apresenta um entalhe mais profundoAmong eucalypt leaf beetles, Metaxyonycha angusta is a very important pest because it causes losses to the wood production in the Brazilian eucalypt plantations. This work deals to the morphologic character which allow sex determination in that insect specie. External morphologic characteristics, such as urosternite shapes and body measurements, were examined from 50 insects of each sex. Sex determination may be found out through examining the fifth urosternite. In males, there is a small clipping at the posterior edge of the fifth urosternite. Females have the posterior edge of that segment presenting a deeper notch

  20. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, Jessica; Verheggen, François; Lognay, Georges; Haubruge, Eric

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and...

  1. Large carrion beetles (Coleoptera, Silphidae) in Western Europe: a review

    OpenAIRE

    Dekeirsschieter, J.; Verheggen, F.; Lognay, G.; Haubruge, E.

    2011-01-01

    This review focuses on carrion beetles (Coleoptera, Silphidae) of the Western Palearctic and their potential use in forensic entomology as bioindicators. Few studies have looked at Silphidae in forensic context and investigations. However, some Silphidae present the desirable characteristics of some Diptera used in postmortem estimates and thus may extend the minimum postmortem interval (PMImin). We review here the taxonomy and distribution of Western Palearctic Silphidae. The anatomical and ...

  2. Nocturnal Migration of Coleoptera: Carabidae in North China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Vertical-looking radar (VLR) has allowed long-term automatic monitoring of the altitudinal and temporal dynamics of high-flying insect populations. To investigate whether ground beetle, insect of Coleoptera, was capable of migrating and its migration pattern by taking advantages of capability of the VLR for long-term real-time automatic monitoring, the migration of Coleoptera ground beetle was investigated by setting up radar observation points, making long-term observation using the VLR and related supplementary equipment, and analyzing low altitude air current and large area circulating current in combination with the meteorological data. Information obtained in 2005 and 2006 showed that the seasonal activities of ground beetles traps of trap lamps were mainly from late June to late August, peak period was mainly in August, seasonal traps of high-altitude lamps and ground lamps were featured by sudden increase and sudden decrease; in peak period, the height of radar echo point could be as high as 600 m, while it was mainly below the height of 450 m; night activities mainly occurred from 20:00 to 22:00, in very few nights, radar echo could last until about 04:00, changes in numbers of ground beetles within the searchlights were consistent with radar echo intensity; ground beetle images were successfully trapped in the sweep nets carried by captive balloons at the height of 200 m. Some species of Carabidaes had some degrees of migration, thus providing the foundation for investigating the migration of Coleoptera insects.

  3. Estados inmaduros de Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini Immature stages of Ancognatha ustulata (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini

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    Jhon César Neita-Moreno

    2008-12-01

    Full Text Available Se describen y se ilustran por primera vez la larva de tercer estadio y la pupa de Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini. Se aportan datos sobre la biología de la especie y su distribución en Colombia. Se proporciona una clave para la identificación de las larvas de tercer estadio conocidas de las especies del género Ancognatha Erichson.The third instar larva and pupa of Ancognatha ustulata Burmeister, 1847 (Coleoptera: Melolonthidae: Dynastinae: Cyclocephalini are described and illustrated for the first time. New data on larval biology and distribution of the species in Colombia are included. A key to the known third stage larvae of Ancognatha Erichson is provided.

  4. Family-Group Names In Coleoptera (Insecta

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    Patrice Bouchard

    2011-04-01

    Full Text Available We synthesize data on all known extant and fossil Coleoptera family-group names for the first time. A catalogue of 4887 family-group names (124 fossil, 4763 extant based on 4707 distinct genera in Coleoptera is given. A total of 4492 names are available, 183 of which are permanently invalid because they are based on a preoccupied or a suppressed type genus. Names are listed in a classification framework. We recognize as valid 24 superfamilies, 211 families, 541 subfamilies, 1663 tribes and 740 subtribes. For each name, the original spelling, author, year of publication, page number, correct stem and type genus are included. The original spelling and availability of each name were checked from primary literature. A list of necessary changes due to Priority and Homonymy problems, and actions taken, is given. Current usage of names was conserved, whenever possible, to promote stability of the classification. New synonymies (family-group names followed by genus-group names: Agronomina Gistel, 1848 syn. n. of Amarina Zimmermann, 1832 (Carabidae, Hylepnigalioini Gistel, 1856 syn. n. of Melandryini Leach, 1815 (Melandryidae, Polycystophoridae Gistel, 1856 syn. n. of Malachiinae Fleming, 1821 (Melyridae, Sclerasteinae Gistel, 1856 syn. n. of Ptilininae Shuckard, 1839 (Ptinidae, Phloeonomini Ádám, 2001 syn. n. of Omaliini MacLeay, 1825 (Staphylinidae, Sepedophilini Ádám, 2001 syn. n. of Tachyporini MacLeay, 1825 (Staphylinidae, Phibalini Gistel, 1856 syn. n. of Cteniopodini Solier, 1835 (Tenebrionidae; Agronoma Gistel 1848 (type species Carabus familiaris Duftschmid, 1812, designated herein syn. n. of Amara Bonelli, 1810 (Carabidae, Hylepnigalio Gistel, 1856 (type species Chrysomela caraboides Linnaeus, 1760, by monotypy syn. n. of Melandrya Fabricius, 1801 (Melandryidae, Polycystophorus Gistel, 1856 (type species Cantharis aeneus Linnaeus, 1758, designated herein syn. n. of Malachius Fabricius, 1775 (Melyridae, Sclerastes Gistel, 1856 (type species

  5. Primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae and Disteniidae) of the Smithsonian Institution

    Science.gov (United States)

    The primary types of longhorned woodboring beetles (Coleoptera: Cerambycidae, Disteniidae) of the National Museum of Natural History (Smithsonian Institution) are catalogued and figured, current through 2012 (but also including some 2013 holotypes). Data on the original combination, current combina...

  6. New records of predaceous diving beetles (Coleoptera:Dytiscidae) in Maine

    Science.gov (United States)

    Boobar, L.R.; Gibbs, K.E.; Longcore, J.R.; Perillo, A.M.

    1996-01-01

    Locations, habitat descriptions, and collection dates are listed for new records of 4 genera and 12 species of predaceous diving beetles (Coleoptera: Dytiscidae) in Maine. Previously, 17 genera and 53 species of the aquatic beetle were reported from Maine.

  7. First record of Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae in Brazil

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    Ubirajara R. Martins

    2015-03-01

    Full Text Available Molorchus minor minor (Linnaeus (Coleoptera, Cerambycidae is recorded for the first time in Brazil (Bahia. It was originally described from Europe and is currently widely distributed in that continent and Asia.

  8. Methods for assessing infestations of sunflower stem weevil (Coleoptera: Curculionidae) in sunflower stems

    Science.gov (United States)

    The sunflower stem weevil, Cylindrocopturus adspersus LeConte (Coleoptera: Curculionidae), reduces sunflower, Helianthus annuus L. (Asteraceae), yields by spreading pathogens, damaging vascular tissues, and promoting lodging of sunflower plants. To assess weevil populations for host plant resistanc...

  9. A new species of the genus Falsoibidion Pic (Coleoptera, Cerambycidae) from Korea

    OpenAIRE

    Lee, Seunghyun; Lee, Seunghwan

    2016-01-01

    A new species of the genus Falsoibidion Pic, 1922 (Coleoptera, Cerambycidae, Cerambycinae, Callidiopini) from Korea is described. Habitus and genitalia of male and female of the new species are illustrated.

  10. Biologi Hama Kumbang Penggerek Pucuk Kelapa Sawit (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) Di Rumah Kassa

    OpenAIRE

    Sejahtra, Ahmad

    2011-01-01

    Ahmad Sejahtra, "BIOLOGY SHOOT BORERS BEETLE PEST OF COCONUT PALM (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) AT KASSA HOUSE", Under supervised by Ms Marheni and Ms Fatima Zahara. This study aims to determine biological pest of oil palm bud weevil (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) at home kassa. The experiment was conducted on people's plantations in PERUMNAS Simalingkar starting from September 2010 until April 2011. Research using observational methods for this resear...

  11. New Curculionoidea (Coleoptera records for Canadа

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    Hume Douglas

    2013-06-01

    Full Text Available The following species of Curculionoidea are recorded from Canada for the first time, in ten cases also representing new records at the generic level: Ischnopterapion (Ischnopterapion loti (Kirby, 1808; Stenopterapion meliloti (Kirby, 1808 (both Brentidae; Atrichonotus taeniatulus (Berg, 1881; Barinus cribricollis (LeConte, 1876; Caulophilus dubius (Horn, 1873; Cionus scrophulariae (Linnaeus, 1758; Cryptorhynchus tristis LeConte, 1876; Cylindrocopturus furnissi Buchanan, 1940; Cylindrocopturus quercus (Say, 1832; Desmoglyptus crenatus (LeConte, 1876; Pnigodes setosus LeConte, 1876; Pseudopentarthrum parvicollis (Casey, 1892; Sibariops confinis (LeConte, 1876; Sibariops confusus (Boheman, 1836; Smicronyx griseus LeConte, 1876; Smicronyx lineolatus Casey, 1892; Euwallacea validus (Eichhoff, 1875; Hylocurus rudis (LeConte, 1876; Lymantor alaskanus Wood, 1978; Phloeotribus scabricollis (Hopkins, 1916; Scolytus oregoni Blackman, 1934; Xyleborus celsus Eichhoff, 1868; Xyleborus ferrugineus (Fabricius, 1801; Xylosandrus crassiusculus (Motschulsky, 1866 (all Curculionidae. In addition the following species were recorded for the first time from these provinces and territories: Yukon – Dendroctonus simplex LeConte, 1868; Phloetribus piceae Swaine, 1911 (both Curculionidae; Northwest Territories – Loborhynchapion cyanitinctum (Fall, 1927 (Brentidae; Nunavut – Dendroctonus simplex LeConte, 1868 (Curculionidae; Alberta – Anthonomus tectus LeConte, 1876; Promecotarsus densus Casey, 1892; Dendroctonus ponderosae Hopkins, 1902; Hylastes macer LeConte, 1868; Rhyncolus knowltoni (Thatcher, 1940; Scolytus schevyrewi Semenov Tjan-Shansky, 1902 (all Curculionidae; Saskatchewan – Phloeotribus liminaris (Harris, 1852; Rhyncolus knowltoni (Thatcher, 1940; Scolytus schevyrewi Semenov Tjan-Shansky, 1902 (all Curculionidae; Manitoba – Cosmobaris scolopacea Germar, 1819; Listronotus maculicollis (Kirby, 1837; Listronotus punctiger LeConte, 1876; Scolytus

  12. Boll weevil (Coleoptera: Curculionidae) survival through cotton gin trash fans.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-10-01

    There is concern that cotton gins may serve as loci for reintroduction of boll weevils, Anthonomus grandis grandis Boheman, to eradicated or suppressed zones when processing weevil-infested cotton from neighboring zones. Previous work has shown that virtually all weevils entering the gin in the seed cotton will be removed before they reach the gin stand. Those not killed by the seed cotton cleaning machinery will be shunted alive into the trash fraction, which passes through a centrifugal trash fan before exiting the gin. The objective of this study was to determine survival potential of boll weevils passed through a trash fan. Marked adult weevils were distributed in gin trash and fed through a 82.6-cm (32.5-in.) diameter centrifugal fan operated across a range of fan-tip speeds. A small number of boll weevils were recovered alive immediately after passage through the fan, but all were severely injured and did not survive 24 h. In another experiment, green bolls infested with both adult- and larval-stage weevils were fed through the fan. Several teneral adults survived 24 h, and there was no evidence that fan-tip speed affected either initial survival of weevils, or the number of unbroken boll locks that could harbor an infesting weevil. Thus, designating a minimum fan-tip speed for ensuring complete kill is not possible for the boll weevil. Experiments suggest that a device installed in a gin that partially crushes or cracks bolls open before entering a trash fan will increase mortality, possibly enough that further precautions would be unnecessary. PMID:15568350

  13. Early-Summer Pheromone Biology of Galerucella calmariensis and Relationship to Dispersal and Colonization

    Science.gov (United States)

    Galerucella calmariensis (Coleoptera: Chrysomelidae) has become an effective biological control agent for purple loosestrife (Lythrum salicaria). A male-produced aggregation pheromone was recently identified in this mostly univoltine beetle, and attractiveness to both sexes was demonstrated in the ...

  14. Papel dos besouros (Insecta, Coleoptera) na Entomologia Forense

    OpenAIRE

    Wellington Emanuel dos Santos

    2014-01-01

    Esse trabalho apresenta uma revisão do papel que os besouros (Insecta, Coleoptera) desempenham na Entomologia Forense. Discussões sobre ocorrência em cadáveres humanos e carcaças animais, estimativas de Intervalo Pós-Morte (IPM), estudos realizados no Brasil e em outros países, principais famílias de importância forense e aspectos biológicos, ecológicos e biogeográficos das espécies são apresentadas.

  15. Fossil history of Mesozoic weevils (Coleoptera:Curculionoidea)

    Institute of Scientific and Technical Information of China (English)

    Andrei A.Legalov

    2012-01-01

    The first synopsis of Mesozoic weevils (Curculionoidea: Coleoptera) is presented.Changes of family,genera and species abundance during the Mesozoic revealed three distributional patterns.The Jurassic (Karatau) fauna was dominated by the Nemonychidae.During the Early Cretaceous (beginning at the Jurassic/Cretaceous border),the Ithyceridae was the prevalent group with a significant role played by the Nemonychidae.In the Late Cretaceous (Cenomanian and Turonian),the major groups were the Curculionidae and Brentidae.Obviously,the change of weevil fauna during this period was due to the expansion of the angiosperms,which provided multiple niches in their vegetative and reproductive organs for weevil development.

  16. An annotated catalogue of the Buprestidae of Iran (Coleoptera: Buprestoidea).

    Science.gov (United States)

    Ghahari, Hassan; Volkovitsh, Mark G; Bellamy, Charles L

    2015-01-01

    An annotated taxonomic catalogue of the jewel beetles (Coleoptera: Buprestidae) of Iran is given. Original descriptions and recent revisionary or catalogue data are included along with the distribution, both within and outside of Iran, ecological data and host plant associations, junior synonyms, and comments. A complete bibliography completes the catalogue. In total 428 species and 52 subspecies of jewel beetles belonging to 6 subfamilies (Julodinae, Polycestinae, Galbellinae, Chrysochroinae, Buprestinae, and Agrilinae), 20 tribes, and 38 genera are known from Iran including doubtful records and 4 nomina nuda. It is likely that the number of jewel beetle species from Iran will be between 460-480 and possibly even more species. PMID:26250020

  17. The complete mitochondrial genome of Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Sun, Tian Yi; Li, Lei; Xin, Tianrong; Wang, Yayu; Xia, Bin

    2016-09-01

    We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA(Ile). In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species. PMID:26330111

  18. Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae

    Directory of Open Access Journals (Sweden)

    Juliana S. Vieira

    2014-09-01

    Full Text Available Rove beetles of medical importance in Brazil (Coleoptera, Staphylinidae, Paederinae. The rove beetles of the genus Paederus Fabricius, 1775 are the most important group within Coleoptera causing dermatitis around the world. The medical importance of Paederus depends on its toxic hemolymph released when these beetles are crushed on human skin. The effects are mainly dermatitis linearis and some sporadic cases of conjunctivitis. In Brazil seven species of Paederus are known to cause dermatitis: P. amazonicus Sharp, 1876, P. brasiliensis Erichson, 1840, P. columbinus Laporte, 1835, P. ferus Erichson, 1840, P. mutans Sharp, 1876, P. protensus Sharp, 1876 stat. rev., and Paederus rutilicornis Erichson, 1840. Paederus mutans and P. protensus are for the first time recorded as of medical importance, whereas the record of P. rutilicornis in Brazil is doubtful. All seven species are redescribed and a dichotomous key is provided. The geographic distributions of all species are documented. The results provided here include the most recent and relevant taxonomic revision of Paederus of the Neotropical region, the first identification key for Brazilian species and the increase of recorded species of medical importance in the world.

  19. Complete mitochondrial genome of Cryptolestes pusillus (Coleoptera: Laemophloeidae).

    Science.gov (United States)

    Li, Lei; Liu, Guanghua; Sun, Tanyi; Xin, Tianrong; Li, Meiyun; Zou, Zhiwen; Xia, Bin

    2016-09-01

    In this study, the complete mitochondrial genome of Cryptolestes pusillus (GenBank accession number KT070713) was sequenced by long PCR and primer walking methods. The total length of mitochondrial DNA is 15 502 bp and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region. The base composition of the genome is A (39.04%), T (37.07%), C (23.4%), and G (14.6%). Except for COI and ATP8 with TCC and ATC as start codon, respectively, the remaining protein-coding genes initiated with the three orthodox start codons. Two complete stop codons (TAA and TAG) and two incomplete stop codons (COIII stop with T and ND5 stop with TA) were used in the protein-coding genes. The A + T-rich region is located between 12s rRNA and tRNA(Ile) with the length of 859 bp. The phylogenetic relationships of Coleoptera species were constructed based on the nucleotide sequences of 13 protein-coding genes of mitogenome using the neighbor-joining method. The molecular-based phylogenetic analysis supported the traditional morphological classification on relationships within Coleoptera species. PMID:26329895

  20. Biology and Management of Billbugs (Coleoptera: Curculionidae) in Turfgrass

    Science.gov (United States)

    Dupuy, Madeleine M.; Ramirez, Ricardo A.

    2016-01-01

    Billbugs (Coleoptera: Curculionidae: Sphenophorus spp.) are a complex of weevil pests affecting turfgrass throughout the United States. Billbug larvae cause damage by feeding in stems, on roots, and on the crowns of turf, causing severe discoloration and eventual plant death. Monitoring efforts have focused on nondestructive pitfall sampling of ground-active billbug adults and on destructive sampling using soil cores for larval stages in the soil. Given the cryptic nature of the susceptible larval stages, billbugs are typically managed by preventive applications of long-residual, systemic insecticides, including neonicotinoids and anthranilic diamides. Despite knowledge of effective management practices including pest-resistant turf varieties, irrigation management, and microbial controls that contribute to an IPM approach, billbug management continues to rely heavily on prophylactic synthetic insecticides. This review will summarize the identification and biology of billbugs and strategies for their management. PMID:27065080

  1. BESOUROS COPRÓFAGOS (COLEOPTERA; SCARABAEIDAE COLETADOS EM PIRACICABA, SP

    Directory of Open Access Journals (Sweden)

    RODRIGUES S.R.

    1998-01-01

    Full Text Available Através do uso de armadilhas "pitfall" iscadas com massa fecal fresca de bovinos, realizou-se a coleta de besouros coprófagos (Coleoptera; Scarabaeidae, durante o período de 15 de abril de 1995 a 17 de fevereiro de 1996, em área de pastagem ao lado de confinamento de bovinos, em Piracicaba, SP. Coletou-se um total de 11 espécies distribuidas nos gêneros Aphodius, Ataenius, Trichillum, Eurysternus, Dichotomius e Canthon. Os besouros coprófagos de comportamento endocoprídeo representaram 72,73% das espécies coletadas, sendo estes os de maior ocorrência e a espécie Aphodius lividus parece ser a mais bem adaptada na área estudada.

  2. Walking stability of Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae).

    Science.gov (United States)

    Pires, E M; Nogueira, R M; Pina, D S; Manica, C L M; Faroni, L R A; Moreira, P S A

    2016-04-19

    Results obtained in studies can contribute to the advancement of science and innovative methods and techniques for developing practical activities. Reporting conditions that may restrict the implementation of research is critical to ensure the optimal development of further technical studies. The objective of this study was to assess the walking stability of R. dominica on a flat and smooth surface. The study was based on the determination of mortality, morphology and walking stability of the insect outside the grain mass, on a flat and smooth surface. Mortality of adults of this Coleoptera in conditions with and without food was similar, which explains the difficulty that this insect had for accessing the food source on the flat and smooth surface. The measurements of body length (BOL), width (BOW) and height (BOH) of R. dominica were compared with those of Tribolium castaneum (Coleoptera: Tenebrionidae), which showed good ability to walk in these conditions. This study indicated that the former presents lower BOL and BOW, and greater BOH than the second, and all these variables showed differences when analyzed simultaneously by means of the construction of multivariate morphometric indices (Width × Height, Length × Height and Height × Length × Width). These morphometric variables, together with the definition of the geometry most similar to the body shape, resulted in determination of the center of gravity (CG) and static rollover threshold (SRTgeom) for both species. Rhyzopertha dominica and T. castaneum presented CGs considered high and low, respectively, and together with the values obtained for SRTgeom, may justify that R. dominica can be considered a less stable species during movement, and presents greater risk of rollover on flat and smooth surfaces. PMID:27097085

  3. Track analysis of the Neotropical Entimini (Coleoptera: Curculionidae: Entiminae

    Directory of Open Access Journals (Sweden)

    Alberto Romo

    2011-09-01

    Full Text Available Track analysis of the Neotropical Entimini (Coleoptera, Curculionidae, Entiminae. Distributional patterns of the species belonging to the tribe Entimini from the Neotropical region were analyzed. Based on a track analysis of 22 species of Entimus, Rhigus, and Phaedropus, for which distributional data were available, two generalized tracks were found. One is located in northern Brazil, corresponding to the Amazonian subregion, and is determined by Phaedropus candidus and Rhigus speciosus. The other is located in southern Brazil, corresponding to the Parana subregion, and is determined by Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus, and R. tribuloides. The development of the Chacoan subregion is hypothesized to have been the dynamic vicariant event that fragmented the former Amazonian-Parana forest.Análisis de trazos de Entimini Neotropical (Coleoptera, Curculionidae, Entiminae. Se analizaron los patrones de distribución de la tribu Entimini en la región Neotropical. Con base en un análisis de los trazos de 22 especies de Entimus, Rhigus y Phaedropus, para los cuales se contaba con datos de distribución, se hallaron dos trazos generalizados. Uno se localiza en el norte de Brasil, en la subregión Amazónica, y está sustentado por Phaedropus candidus y Rhigus speciosus. El otro se localiza en el sur de Brasil, en la subregión Paranaense, y está sustentado por Entimus imperialis, E. excelsus, Phaedropus togatus, Rhigus dejeanii, R. faldermanni, R. horridus, R. lateritus, R. nigrosparsus y R. tribuloides. Se hipotetiza que el desarrollo de la subregión Chaqueña constituyó el evento de vicarianza dinámica que fragmentó el antiguo bosque Amazónico-Paranaense.

  4. Selective breeding for increased pheromone production in the boll weevil (Coleoptera: Curculionidae)

    International Nuclear Information System (INIS)

    The male boll weevil, Anthonomus grandis grandis Boheman, uses an aggregating pheromone to attract females, after which mating often occurs. Sterile boll weevil release programs depend upon this phenomenon to produce sterile matings with feral females. In an effort to increase the effectiveness of the individual sterile male and thereby reduce the number of sterile males required per hectare, a selective-breeding system was used to increase the total pheromone produced by individual male boll weevils. This breeding program increased the total pheromone production by individual male boll weevils to 4.5 times that of the parent population. After irradiation-induced sterilization, there remained 2.2 times more pheromone produced by the selected strain. Therefore, these sterile weevils should be about 2.2 times more attractive to feral females than the parent weevils now in use, and they have the potential to reduce the number of sterile males required in a sterile release program

  5. Bio-edafology of the Coleoptera order, in three Colombia Natural Regions

    International Nuclear Information System (INIS)

    The characterization of Coleoptera families is showed to three different Colombian Natural Regions. This is given in relation with biological and environmental factors, different vegetation covers and soil uses. In addition to it, susceptible Coleoptera taxa are determined when natural conditions are disturbed. Methodically, a literature subject research was made, and Barber and Berlesse traps were used to organism extractions from superficial and under superficial soil. Horizons diversity, riches and constancy index were determined to each family. Results show different diversity, riches and constancy values to each family, as much in each natural region, such as to each soil use

  6. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae)

    OpenAIRE

    Dilma Solange Napp; Ubirajara Ribeiro Martins

    2006-01-01

    Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae). Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae). The following new genus and species, all from Bolivia, are describ...

  7. Influence of growing location and cultivar on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) infestation of rough rice

    Institute of Scientific and Technical Information of China (English)

    FRANK H. ARTHUR; RUSTY C. BAUTISTA; TERRENCE J. SIEBENMORGEN

    2007-01-01

    Long-grain rice cultivars Cocodrie, Wells, and XP 723 grown in three locations (Hazen, MO; Essex and Newport, AR, USA), and medium-grain rice cultivars Bengal and XP 713 grown in two locations (Jonesboro and Lodge Corner, AR, USA), were harvested and assayed for susceptibility to Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), the lesser grain borer, and Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the rice weevil, on rice held at 27℃ 57% and 75% relative humidity (RH).Separate samples from the same harvest lots were also analyzed for the physical characteristics of brown rice yield, percentage whole kernels and kernel thickness. Progeny production and feeding damage of R. dominica were significantly different among long-grain cultivars within two of the three locations (P < 0.05), but not for location or RH (P ≥ 0.05), while progeny production of S. oryzae was different among cultivars, location, and RH (P < 0.05). On medium-grain rice, both cultivar and location were significant for progeny production of R. dominica, but not RH, while cultivar and RH were significant for progeny production of S. oryzae, but not location. On both rice types, feeding damage of R. dominica followed the same trends and was always strongly positively correlated with progeny production (P < 0.05), but for S. oryzae there were several instances in which progeny production was not correlated with feeding damage (P ≥ 0.05). Physical characteristics of both rice types were statistically significant (P < 0.01) but actual numerical differences were extremely small, and were generally not correlated with progeny production of either species. Results indicate that the location in which a particular rice cultivar is grown, along with its characteristics, could affect susceptibility of the rice to R. dominica and S. oryzae.

  8. "Blister Beetles (Coleoptera: Meloidae in Nahavand County (Hamedan Province, Iran and Their Ecological Relationship to Other Coleopteran Families"

    Directory of Open Access Journals (Sweden)

    MR Nikbakhtzadeh

    2002-08-01

    Full Text Available Field collection in Nahavand county (Hamedan Province, Iran revealed 9 blister beetle (Col: Meloidae species from three different tribes of subfamily Meloinae. In tribe Mylabrini, Mylabris impressa Chevrolat 1837, Mylabris schreibersi Reiche 1865, Mylabris variabilis (Pallas, 1781, Mylabris guerini Chevrolat 1837, Lydoceras bilineatus Thomas 1897 and Croscherichia spp. Pardo Alcaide identified; whereas in tribe Lyttini Alosimus smyrnensis (Maran 1942 and Muzimes iranicus (Maran 1942 found. Another species was Calydos alloushei Kaszab 1960 of tribe Eupomphini. Two records of Mylabris impressa and Mylabris schreibersi are quite new for Iranian fauna. There are some interesting mimicry rings between meloid species and 8 species of other coleopteran families which indicates a remarkable Müllerian mimicry. Mimics of the following families have taken advantage of Meloid aposematism towards a better natural fitness: Cerambycidae, Cleridae, Pedilidae, Melyridae, Chrysomelidae, Cantharidae and Cicindelidae. Canthariphily of families Chrysomelidae, Cantharidae and Cerambycidae are new reports which have never been shown elsewhere.

  9. Parasitylenchus sp. (Tylenchomorpha: Allantonematidae) parasitizing field populations of Harmonia axyridis (Coleoptera: Coccinellidae)

    DEFF Research Database (Denmark)

    Harding, Susanne; Poinar, George O. Jr.; Dimitrova, Desislava V.; Steenberg, Tove

    2011-01-01

    Adults of the invasive harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae), were found to be parasitized by nematodes (Tylenchomorpha: Allantonematidae) in Denmark. The nematodes were identified as Parasitylenchus sp. Major morphological characters of the nematodes did not differ si...

  10. Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae), information on a little known taxon from Colombia

    OpenAIRE

    Kippenhan, Mike

    2011-01-01

    Two specimens of Oxycheila binotata Gray (Coleoptera: Carabidae: Cicindelinae) in the Field Museum of Natural History offer additional information on the morphology and distribution of this rare species. One of the specimens, a female, is considered to be the first known specimen of this species.

  11. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae from Korea

    Directory of Open Access Journals (Sweden)

    Ik Je Choi

    2016-03-01

    Full Text Available A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  12. Patterns of tree species usage by long-horned beetles (Coleoptera: Cerambycidae) in Fiji

    Czech Academy of Sciences Publication Activity Database

    Waqa-Sakiti, H.; Stewart, A.; Čížek, Lukáš; Hodge, S.

    2014-01-01

    Roč. 68, č. 1 (2014), s. 57-64. ISSN 0030-8870 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera Subject RIV: EH - Ecology, Behaviour Impact factor: 0.924, year: 2014 http://www.bioone.org/doi/pdf/10.2984/68.1.5

  13. An illustrated checklist of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve, Kerala, India

    Directory of Open Access Journals (Sweden)

    Nithya Sathiandran

    2015-12-01

    Full Text Available An illustrated checklist of 36 species of dung beetles (Coleoptera: Scarabaeinae from the Periyar Tiger Reserve in the southern Western Ghats is presented.  Records of eight species endemic to the Western Ghats and a rare primitive old world dung beetle group, Ochicanthon nitidus (Paulian, from the forests of Periyar Tiger Reserve are provided. 

  14. Study on the genus Daptus ground-beetles (Coleoptera: Carabidae) from Korea

    OpenAIRE

    Ik Je Choi; Jongok Lim; Jinyoung Park; Ji Hwan Park; Jong Kyun Park

    2016-01-01

    A genus Daptus Fischer von Waldheim, 1823 of the tribe Harpalini Bonelli, 1810 (Coleoptera: Carabidae) is reported for the first time from Korea, based on the Daptus vittatus Fischer von Waldheim from Incheon, Korea. Redescription of the species and illustrations of diagnostic characteristics, including genitalia characteristics of both sexes, are provided.

  15. Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in Interior Alaska

    Science.gov (United States)

    Adult ground beetles (Coleoptera: Carabidae) abundance and diversity were documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145º W). Twenty species were documented based on a total sample of 6,116 specimens collected during 2006 and 2007. Two speci...

  16. Multistate characters and diet shifts: evolution of Erotylidae (Coleoptera).

    Science.gov (United States)

    Leschen, Richard A B; Buckley, Thomas R

    2007-02-01

    The dominance of angiosperms has played a direct role in the diversification of insects, especially Coleoptera. The shift to angiosperm feeding from other diets is likely to have increased the rate of speciation in Phytophaga. However, Phytophaga is only one of many hyperdiverse lineages of beetles and studies of host-shift proliferation have been somewhat limited to groups that primitively feed on plants. We have studied the diet-diverse beetle family Erotylidae (Cucujoidea) to determine if diet is correlated with high diversification rates and morphological evolution by first reconstructing ancestral diets and then testing for associations between diet and species number and diet and ovipositor type. A Bayesian phylogenetic analysis of morphological data that was previously published in Leschen (2003, Pages 1-108 in Fauna of New Zealand, 47; 53 terminal taxa and 1 outgroup, 120 adult characters and 1 diet character) yielded results that are similar to the parsimony analyses of Leschen (2003). Ancestral state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed microfungal feeding (the diet of the outgroup Biphyllidae) at the root of the Erotylidae tree. Shifts among microfungal, saprophagous, and phytophagous diets were most frequent. The largest numbers of species are contained in lineages that are macrofungal feeders (subfamily Erotylinae) and phytophagous (derived Languriinae), although the Bayesian posterior predictive tests of character state correlation were unable to detect any significant associations. Ovipositor morphology correlated with diet (i.e., acute forms were associated with phytophagy and unspecialized forms were associated with a mixture of diets). Although there is a general trend to increased species number associated with the shift from microfungal feeding to phytophagy (based on character mapping and mainly restricted to shifts in Languriinae), there is a large radiation of taxa feeding on

  17. 莲草直胸跳甲生殖系统与繁殖特性研究%The reproductive system and reproductive biology of the alligatorweed flea beetle,Agasicles hygrophila (Coleoptera:Chrysomelidae)

    Institute of Scientific and Technical Information of China (English)

    陈磊; 蔡笃程; 陈青; 唐超; 冯岗; 彭正强; 金启安; 温海波

    2009-01-01

    为了解与莲草直胸跳甲Agasicles hygrophila繁殖密切相关的结构与行为特性,结合显微解剖和室内观察,对该跳甲雌雄成虫的生殖系统构造、雌成虫卵巢发育进度以及繁殖特性进行了研究.结果表明:莲草直胸跳甲雌性生殖系统包括卵巢、侧输卵管、中输卵管、受精囊及其附腺,左右卵巢一般不对称,单侧有12~16根卵巢管,受精囊豆芽状,卵巢管为端滋式;雄性生殖系统由睾丸、侧输精管及附腺、射精管和阳茎及附属器官组成,阳基叉式.根据相关形态特征,卵巢发育进度可分为发育初期(1级)、卵黄沉积前期(Ⅱ级)、成熟待产期(Ⅲ级)、产卵盛期(Ⅳ级)和产卵末期(V级)5个级别,各等级在卵巢分区长度(原卵区、生长区和成熟区)及怀卵量上存在显著差异.成虫羽化2 d后即可进行交配,16:00-18:00时为交配高峰期,雌成虫产卵高峰期12:00-16:00时,且偏好在寄主植株中部偏上叶片背部产卵;22-32℃时,雌性成虫的寿命和产卵总量随温度的上升而逐渐下降,25-30℃间差异不显著,但32℃时产卵前期延长至7.2 d,寿命和产卵量显著下降,表明32℃不利于莲草直胸跳甲繁殖.因此,推测32℃及以上持续高温造成的子代卵量急剧减少,可能是该跳甲夏季田间种群数量下降的原因之一.%In order to identify organs and behavior closely related to reproduction, observations on reproductive system and reproductive biology of the alligatorweed flea beetle, Agasicles hygrophila were conducted in the laboratory. The results showed that the female adult have 2 ovaries, each of which contains 12 -16 telotrophic ovarioles, spermatheca is in the shape of bean sprout, and the aedeagus is fork-type. Ovarian development can be divided into 5 grades based on the morphological characteristics of ovaries: stage Ⅰ (No oocyte stage), stage Ⅱ (Previtellogenic stage), stage Ⅲ (Egg maturation stage), stage Ⅳ (Ovipositing stage), and stage V (Post-oviposition stage). Each stage shows significant differences in the length of ovarian zones and egg-carrying number. In addition, the beetles could mate around 2 d after emergence, a copulation peak appears on 16:00-18:00, the oviposition peak occurrs on 12:00-16:00, and the beetles prefer to the blade back of leaf in the middle stratum of host. Temperature had a significant effect on longevity and fecundity. Female longevity and fecundity decreased with increasing of the temperature within 22-32℃, and temperatures below 22℃ or above 32℃ were unfavorable for ovarian development. At 32℃, the pre-oviposition period extended to 7. 2 d, while the longevity and fecundity decreased significantly. Therefore, it is inferred that high and constant temperature above 32℃ is a possible cause for the population decay of the alligatorweed flea beetle in summer.

  18. Open field host selection and behavior by tamarisk beetles (Diorhabda spp.)(Coleoptera: Chrysomelidae) in biological control of exotic saltcedars (Tamarix spp.) and risks to non-target athel (T. aphylla) and native Frankenia

    Science.gov (United States)

    Biological control of exotic, invasive saltcedars (Tamarix spp.) in the western USA involves releases of exotic saltcedar leaf beetles, Diorhabda elongata Brullé sensu lato. Adults in cages alight, feed and oviposit on athel (Tamarix aphylla), an evergreen cold-intolerant tree used for shade and as...

  19. Larval performance of the mustard leaf beetle (Phaedon cochleariae, Coleoptera, Chrysomelidae) on white mustard (Sinapis alba) and watercress (Nasturtium officinale) leaves in dependence of plant exposure to ultraviolet radiation

    Energy Technology Data Exchange (ETDEWEB)

    Reifenrath, Kerstin, E-mail: reifenrath@biozentrum.uni-wuerzburg.d [Universitaet Wuerzburg, Julius-von-Sachs Institut fuer Biowissenschaften, Julius-von-Sachs Platz 3, D-97082 Wuerzburg (Germany); Mueller, Caroline, E-mail: caroline.mueller@uni-bielefeld.d [Universitaet Wuerzburg, Julius-von-Sachs Institut fuer Biowissenschaften, Julius-von-Sachs Platz 3, D-97082 Wuerzburg (Germany)

    2009-07-15

    Short-term exposure to ambient or attenuated ultraviolet (UV) radiation resulted in shifts in plant metabolite concentrations of the Brassicaceae Sinapis alba and Nasturtium officinale. Leaf quality also varied between plant species and within species due to age. Larvae of the oligophagous leaf beetle Phaedon cochleariae were raised on these different host leaves, in order to investigate the effects of variable plant chemistry on this herbivore. The performance of P. cochleariae was influenced by chemical differences between and within plant species but it responded with high plasticity to plants stressed by ultraviolet radiation. Body mass increase and developmental times of larvae were exclusively affected by plant species and leaf-age. However, developmental differences were fully compensated in the pupal stage. We suggest that the plasticity of herbivores may depend on the degree of specialisation, and insect performance may not necessarily be altered by stress-induced host plants. - The larval performance of an oligophagous leaf beetle is influenced by chemical differences between and within plant species but responds with high plasticity to plants stressed by ultraviolet radiation.

  20. Invasive Bruchid species Bruchidius siliquastri Delobel, 2007 and Megabruchidius tonkineus (Pic, 1914) (Insecta: Coleoptera: Chrysomelidae: Bruchinae) new in the fauna of Serbia: Review of the distribution, biology and host plants

    OpenAIRE

    Gavrilović, Bojan; Savić, Dragiša

    2013-01-01

    Two invasive bruchid species - Bruchidius siliquastri Delobel, 2007 and Megabruchidius tonkineus (Pic, 1914) - found on Mt. Fruška Gora during 2011 and 2012 were recorded for the first time in Serbian fauna. Originating from Asia, these beetles were accidentally introduced into Europe. Data on their introduction into Serbia, distribution, biology and host plant associations are presented and discussed.

  1. Bazı Bitkilerin Hekzan, Ethanol ve Methanollü Ekstraktlarının Leptinotarsa decemlineata SAY (Coleoptera:Chrysomelidae)'nın Farklı Dönemleri Üzerine Kontakt Toksisiteleri

    OpenAIRE

    Sibel YORULMAZ SALMAN; KARA, Nimet; Oktay ÖZ

    2015-01-01

    Bu çalışmada, Ocimum basilicum L., Thymus vulgaris L., Mentha spicata L., Melissa officinalis L. ve Matricaria chamomilla L. bitkilerinin hekzan, ethanol ve methanollü ekstraktlarının Leptinotarsa decemlineata'nın değişik dönemleri üzerindeki kontakt toksisiteleri araştırılmıştır. Her üç çözücüde hazırlanan bitki ekstraktlarının tamamı patates böceğinin ergin dönemine göre 3. ve 4. larva dönemlerinde daha etkili bulunmuştur. Ayrıca çalışmada kullanılan bitkilerin ethanollü ve methanollü ekstr...

  2. Mating Success, Longevity, and Fertility of Diabrotica virgifera virgifera LeConte (Chrysomelidae: Coleoptera in Relation to Body Size and Cry3Bb1-Resistant and Cry3Bb1-Susceptible Genotypes

    Directory of Open Access Journals (Sweden)

    Bryan Wade French

    2015-11-01

    Full Text Available Insect resistance to population control methodologies is a widespread problem. The development of effective resistance management programs is often dependent on detailed knowledge regarding the biology of individual species and changes in that biology associated with resistance evolution. This study examined the reproductive behavior and biology of western corn rootworm beetles of known body size from lines resistant and susceptible to the Cry3Bb1 protein toxin expressed in transgenic Bacillus thuringiensis maize. In crosses between, and within, the resistant and susceptible genotypes, no differences occurred in mating frequency, copulation duration, courtship duration, or fertility; however, females mated with resistant males showed reduced longevity. Body size did not vary with genotype. Larger males and females were not more likely to mate than smaller males and females, but larger females laid more eggs. Moderately strong, positive correlation occurred between the body sizes of successfully mated males and females; however, weak correlation also existed for pairs that did not mate. Our study provided only limited evidence for fitness costs associated with the Cry3Bb1-resistant genotype that might reduce the persistence in populations of the resistant genotype but provided additional evidence for size-based, assortative mating, which could favor the persistence of resistant genotypes affecting body size.

  3. Mating Success, Longevity, and Fertility of Diabrotica virgifera virgifera LeConte (Chrysomelidae: Coleoptera) in Relation to Body Size and Cry3Bb1-Resistant and Cry3Bb1-Susceptible Genotypes.

    Science.gov (United States)

    French, Bryan Wade; Hammack, Leslie; Tallamy, Douglas W

    2015-01-01

    Insect resistance to population control methodologies is a widespread problem. The development of effective resistance management programs is often dependent on detailed knowledge regarding the biology of individual species and changes in that biology associated with resistance evolution. This study examined the reproductive behavior and biology of western corn rootworm beetles of known body size from lines resistant and susceptible to the Cry3Bb1 protein toxin expressed in transgenic Bacillus thuringiensis maize. In crosses between, and within, the resistant and susceptible genotypes, no differences occurred in mating frequency, copulation duration, courtship duration, or fertility; however, females mated with resistant males showed reduced longevity. Body size did not vary with genotype. Larger males and females were not more likely to mate than smaller males and females, but larger females laid more eggs. Moderately strong, positive correlation occurred between the body sizes of successfully mated males and females; however, weak correlation also existed for pairs that did not mate. Our study provided only limited evidence for fitness costs associated with the Cry3Bb1-resistant genotype that might reduce the persistence in populations of the resistant genotype but provided additional evidence for size-based, assortative mating, which could favor the persistence of resistant genotypes affecting body size. PMID:26569315

  4. Tolerance of eCry3.1Ab in Reciprocal Cross Offspring of eCry3.1Ab-Selected and Control Colonies of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae).

    Science.gov (United States)

    Geisert, Ryan W; Ellersieck, Mark R; Hibbard, Bruce E

    2016-04-01

    Two new insect colonies were created by separating virgin western corn rootworm, Diabrotica virgifera virgifera LeConte, males and females from both a selected laboratory colony that was being reared on eCry3.1Ab-expressing corn (Zea mays L.) and a control colony reared on its near-isoline corn. Females from the selected colony were paired with males of the control colony and vice versa to create both a selected female by control male colony (Sel♀) and control female by selected male colony (Con♀). Both colonies along with their parental colonies (eCry3.1Ab-selected and control) were evaluated on eCry3.1Ab-expressing corn and its near-isoline in seedling assays. Larvae from each colony were also used in diet toxicity experiments in order to determine the LC50 and EC50 values for the eCry3.1Ab toxin for each. Statistical analysis of seedling assay experiments did not indicate any significant colony×corn interaction but did show a significant main effect of corn type for both larval recovery and larval head capsule widths. Results from the diet toxicity assays showed the control colony to have a significantly lower LC50 value than the selected and cross colonies and a significantly lower EC50 than the selected and Con♀ colonies. Calculations of dominance values (h) of eCry3.1Ab resistance traits from seedling assays indicated that the two reciprocal cross colonies have a dominance value (h) of ∼1, suggesting dominance of the eCry3.1Ab resistance trait. PMID:26628501

  5. Effects of Larval Feeding Plants on Oviposition Selection in Ophraella communa (Coleoptera:Chrysomelidae)%广聚萤叶甲幼虫取食植物对产卵选择行为的影响

    Institute of Scientific and Technical Information of China (English)

    陆凉夏; 孟玲; 李保平

    2014-01-01

    Oviposition selection of plants is a critical trait in evaluation of host specificity in classical biological weed control. The non-native leafbeetle Ophraella communa (LeSage), an oligophagous insect and effective biological control agent of the alien invasive common ragweed Ambrosia artemisiifolia L. in China, can occasionally feed the non-target cocklebur Xanthium sibiricum Patr. The effects of larval food plants A. artemisiifolia and X. sibiricum on adult selection of oviposition plants were assessed using a repeated measure design in outdoor slat house. In the no-choice trial, when oviposition plant was the same as larval food plant, the number of eggs deposited was not significantly different, but when the larvae were fed on A. artemisiifolia or X. sibiricum, more eggs were deposited on X. sibiricum than on A. artemisiifolia, averaging 24.39 and 25.12 eggs on X. sibiricum, and 18.94 and 21.90 eggs on A. artemisiifolia. Egg size (measured as volume) showed no significant difference between oviposition plants, but was significantly different between larval food plants, being bigger when larvae were fed on A. artemisiifolia than on X. sibiricum. There was no significant difference in egg hatching rate between larval food plants or between oviposition plants, ranging from 60%to 70%. In choice trials, there were no significant effects of larval food plants on oviposition plant preferences in adults. The results of this study suggested that larval food plants may have little influence on oviposition plant selection and number of eggs deposited.%产卵选择性是衡量植食性昆虫寄主专一性的主要性状之一。为揭示广聚萤叶甲幼虫取食的植物是否影响其成虫产卵植物选择性,于室外网室中采用重复观测设计开展试验,研究幼虫期取食靶标豚草或非靶标苍耳对成虫在这2种植物上的产卵选择性。在非选择性试验中,广聚萤叶甲幼虫取食豚草和苍耳后的产卵数量没有显著差异;但成虫在2种植物上的产卵数量存在显著差异,如幼虫分别在豚草和苍耳上取食,其成虫在豚草上分别产18.94和21.90粒卵,而在苍耳上分别产24.39和25.12粒卵。对卵粒大小(用体积表示)的分析表明,产卵植物之间及其与幼虫取食植物互作对卵粒大小均无显著影响,但幼虫取食不同植物的成虫所产卵粒大小存在显著差异,幼虫取食豚草的成虫产卵粒显著大于取食苍耳的产卵粒。卵孵化率为60%~70%,在各处理之间没有显著差异。在选择性试验中,对产卵选择频次的分析未发现幼虫取食不同植物以及成虫产卵测试植物等处理影响产卵选择偏好。本研究结果说明,广聚萤叶甲雌成虫产卵数量决策行为受幼虫取食植物的影响不大。

  6. Larval performance of the mustard leaf beetle (Phaedon cochleariae, Coleoptera, Chrysomelidae) on white mustard (Sinapis alba) and watercress (Nasturtium officinale) leaves in dependence of plant exposure to ultraviolet radiation

    International Nuclear Information System (INIS)

    Short-term exposure to ambient or attenuated ultraviolet (UV) radiation resulted in shifts in plant metabolite concentrations of the Brassicaceae Sinapis alba and Nasturtium officinale. Leaf quality also varied between plant species and within species due to age. Larvae of the oligophagous leaf beetle Phaedon cochleariae were raised on these different host leaves, in order to investigate the effects of variable plant chemistry on this herbivore. The performance of P. cochleariae was influenced by chemical differences between and within plant species but it responded with high plasticity to plants stressed by ultraviolet radiation. Body mass increase and developmental times of larvae were exclusively affected by plant species and leaf-age. However, developmental differences were fully compensated in the pupal stage. We suggest that the plasticity of herbivores may depend on the degree of specialisation, and insect performance may not necessarily be altered by stress-induced host plants. - The larval performance of an oligophagous leaf beetle is influenced by chemical differences between and within plant species but responds with high plasticity to plants stressed by ultraviolet radiation.

  7. 叶甲亚科一新属及两新种记述(鞘翅目:叶甲科)%A new genus and two new species of Chrysomelinae (Coleoptera: Chrysomelidae) from China

    Institute of Scientific and Technical Information of China (English)

    葛斯琴; DACCORDI Mauro; LOPATIN Igor K.; 崔俊芝; 李文柱; 杨星科

    2012-01-01

    A new leaf beetle genus Yulongedon gen.nov.,and two new species,Y.formosus sp.nov.and Y.jambhalai sp.nov.from China,are described and illustrated here.%记述中国叶甲亚科1新属:玉龙叶甲属Yulongedon gen.nov.,包括2新种:靓玉龙叶甲Y.formosu sp.nov.,宝藏神玉龙叶甲Y.jambhalai sp.nov..

  8. Bazı Bitkilerin Hekzan, Ethanol ve Methanollü Ekstraktlarının Leptinotarsa decemlineata SAY (Coleoptera:Chrysomelidae'nın Farklı Dönemleri Üzerine Kontakt Toksisiteleri

    Directory of Open Access Journals (Sweden)

    Sibel YORULMAZ SALMAN

    2015-03-01

    Full Text Available In this study, the contact toxicities of the extracts with hexane, ethanol Leptinotarsa decemlineata. Whole of the plant extracts in all of three solvents were found more effective in third and forth larvae period of Leptinotarsa decemlineata than adult period. Furthermore, the contact toxicity of the extracts of the plants used in the study with ethanol and methanol were found higher compared to the extracts with hexane in potato beetle. In the study, the highest influence was obtained from the extracts of Thymus vulgaris L. plant with methanol. This extract demonstrated 96.45%-influence on the third-period larvae of potato beetle, 85.70%-influence on fourth-period larvae of potato beetle and 53.50%-influence on adult period. The lowest influence in the study was identified as 32.18% in third-period larvae of potato beetle, 24.10% in fourth-period larvae of potato beetle and 10.70% in adult period in chamomile extract with hexane

  9. Spatio-temporal distribution of Cœlaenomenodera minuta Uhmann (Coleoptera: Chrysomelidae), a serious insect pest of oil palm (Elaeis guineensis Jacq.) in the south-west region of Cameroon

    OpenAIRE

    Mondjeli Constantin; Godswill Ntsomboh Ntsefong; Ngando Ebongue Georges Frank; Walter Ajambang Nchu; Ignatius Amah Parh; Dibog Luc

    2013-01-01

    The leaflet miner Cœlaenomenodera minuta is the main pest of oil palm in the south-west region of Cameroon. A 12 months study of spatio-temporal distribution was carried out on young and mature industrial plantations of 40 ha each at Tiko Benoe palm estate in the south-west region of Cameroon. The pest infestation (larvae and adult) distribution revealed the endemic existence of C. minuta in the mature oil palm plantation. Relative null pest infestations were recorded from the young plantatio...

  10. Spatio-temporal distribution of Cœlaenomenodera minuta Uhmann (Coleoptera: Chrysomelidae, a serious insect pest of oil palm (Elaeis guineensis Jacq. in the south-west region of Cameroon

    Directory of Open Access Journals (Sweden)

    Mondjeli Constantin

    2013-09-01

    Full Text Available The leaflet miner Cœlaenomenodera minuta is the main pest of oil palm in the south-west region of Cameroon. A 12 months study of spatio-temporal distribution was carried out on young and mature industrial plantations of 40 ha each at Tiko Benoe palm estate in the south-west region of Cameroon. The pest infestation (larvae and adult distribution revealed the endemic existence of C. minuta in the mature oil palm plantation. Relative null pest infestations were recorded from the young plantation. Three infestation peaks were observed. Monthly significant difference of C. minuta infestation was also recorded. The highest number of insects captured (117.3 per tree was in December. In addition, negative and relatively significant correlation was observed between monthly cumulative rainfall days and captured C. minuta individuals. These results can help to improve the conception and the implementation of an efficient control strategy against the pest.

  11. Biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos: um ensaio biogeográfico / Biomass of phototactic Dynastinae (Coleoptera, Scarabaeidae: a biogeographical assay

    Directory of Open Access Journals (Sweden)

    Paulo Jorge Riehs

    2006-04-01

    Full Text Available São apresentados e analisados valores da biomassa de Dynastinae (Coleoptera, Scarabaeidae fototácticos coletados em seis localidades do Leste e Centro-Oeste do Paraná, sul do Brasil, demonstrando-se as diferenças entre as diversas comunidades. Os dados obtidos no Paraná são correlacionados com os de Santa Maria, Rio Grande do Sul.

  12. Desarrollo de un prototipo de formulación con hongos entomopatógenos para el manejo de Demotispa neivai Bondar (Coleoptera: Chrysomelidae) / Development of a prototype of making with entomopathogenic fungi for management of Demotispa neivai Bondar (Coleptera: Chrysomelidae)

    OpenAIRE

    Martínez Castrillón, Luis Carlos

    2010-01-01

    Entre los factores que limita la producción en el cultivo de la palma de aceite se encuentra el insecto Demotispa neivai Bondar, el cual ocasiona daños hasta de un 100% en su superficie. Durante el desarrollo de un prototipo de formulación con hongos entomopatógenos para el manejo de D. neivai fue necesario evaluar la patogenicidad de tres aislamientos de hongos entomopatógenos en adultos del insecto, utilizando como inóculo dos cepas de Beauveria bassiana (B018, B025), y una de Metarhizium a...

  13. Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans.

    Science.gov (United States)

    Sim, Sheina B; Yoneishi, Nicole M; Brill, Eva; Geib, Scott M; Follett, Peter A

    2016-02-01

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei. PMID:26487745

  14. NON PREFERENTIAL OVIPOSITION OF Zabrotes subfasciatus IN COMMON BEANS (Phaseolus vulgaris L. APPLIED WITH SOME VEGETAL PRODUCTS NÃO-PREFERÊNCIA PARA OVOPOSIÇÃO DE Zabrotes subfasciatus (Boheman, 1833 EM FEIJÃO TRATADO COM DIFERENTES PRODUTOS DE ORIGEM VEGETAL

    Directory of Open Access Journals (Sweden)

    Corival Cândido da Silva

    2007-09-01

    Full Text Available

    It was studied the non-preferential oviposition of Zabrotes subfasciatus (Boheman, 1833 in common beans (P. vulgaris L. cv. carioca applied with some vegetal products. The applied products were andiroba oil (Carapa guianensis, neem oil (Azadirachta indica, neem solution and the commercial product Azatin with rates 2, 4 and 6 ml/kg grain. The experimental design was completely randomized in factorial scheme 4 x 4 with 5 replications. Data were transformed in (x + 1^1/2 and variance analysis while averages were evaluated by Tukey test 5%. All products differed in control. Neem solution and Azatin at 4 ml/kg grain, neem oil at 6 ml/kg grain and andiroba oil (2, 4 and 6 ml/kg grain showed better results than other treatments.

    KEY-WORDS: Zabrotes; Phaseolus; Carapa; Azadirachta; resistance.

    Foi estudada a não-preferência para a oviposição de Zabrotes subfasciatus (Boheman,1833 em feijão (Phaseolus vulgaris cultivar carioca, tratado com

  15. Suscetibilidade de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae ao enxofre Susceptibility of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae to sulfur

    Directory of Open Access Journals (Sweden)

    José Roberto Gonçalves

    2007-08-01

    Full Text Available As criações de Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae são freqüentemente infestadas pelo ácaro Acarophenax lacunatus (Cross e Krantz (Prostigmata: Acarophenacidae. O objetivo deste trabalho foi avaliar doses de enxofre, acaricida eficaz contra A. lacunatus, não-prejudiciais ao desenvolvimento de R. dominica. As unidades experimentais foram constituídas de placas de Petri contendo 30 g de grãos de trigo infestados com 30 adultos de R. dominica. Os tratamentos consistiram na utilização de doses de enxofre sobre os grãos, correspondentes a 0,0; 0,6; 0,9; 1,2; 1,5; 3,0; 6,0; 12,0; 24,0 e 48,0mg i a g-1, em dez repetições. As unidades experimentais foram armazenadas por 60 dias a 30±1°C, 60±5% UR e escotofase de 24h. O desenvolvimento de R. dominica foi afetado pela utilização de doses de enxofre maiores que 3,0mg i a g-1.The laboratory rearing of Rhyzopertha dominica (Fabricius (Coleoptera: Bostrichidae is frequently infested by the parasite mite Acarophenax lacunatus (Cross and Krantz (Prostigmata: Acarophenacidae. This study was aimed at evaluating the sulfur doses, an effective acaricide against A. lacunatus, not harmful to the development of with R. dominica. The experimental units were Petri dishes containing 30g of whole wheat grains powdered with the different doses of the sulfur (0.0, 0.6, 0.9, 1.2, 1.5, 3.0, 6.0, 12.0, 24.0 and 48.0mg a i g-1 infested with 30 adults of R. dominica, in ten replicates. All treatments were maintained under controlled conditions (30±1°C, 60±5% r h and 24h scotophase for 60 days after the insect infestation. Sulfur doses higher than 3.0mg a i g-1 negatively affected R. dominica development.

  16. The complete mitogenome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae).

    Science.gov (United States)

    Nan, Xiaoning; Wei, Cong; He, Hong

    2016-05-01

    The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%. PMID:25427809

  17. Plant compounds insecticide activity against Coleoptera pests of stored products

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Marcio Dionizio; Picanco, Marcelo Coutinho; Guedes, Raul Narciso Carvalho; Campos, Mateus Ribeiro de; Silva, Gerson Adriano; Martins, Julio Claudio [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Biologia Animal]. E-mail: marcio.dionizio@gmail.com; picanco@ufv.br; guedes@ufv.br; mateusc3@yahoo.com.br; agronomiasilva@yahoo.com.br

    2007-07-15

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on R. dominica was evaluated. Among them, only hexane extract of A. conyzoides showed insecticide activity; the hexane extract of this species was successively fractionated by silica gel column chromatography, for isolation and purification of the active compounds. Compounds 5,6,7,8,3',4',5'-heptamethoxyflavone; 5,6,7,8,3'-pentamethoxy-4',5'-methilenedioxyflavone and coumarin were identified. However, only coumarin showed insecticide activity against three insect pests (LD{sub 50} from 2.72 to 39.71 mg g{sup -1} a.i.). The increasing order of insects susceptibility to coumarin was R. dominica, S. zeamais and O. surinamensis. (author)

  18. Economic analysis of emerald ash borer (Coleoptera: Buprestidae) management options.

    Science.gov (United States)

    Vannatta, A R; Hauer, R H; Schuettpelz, N M

    2012-02-01

    Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), plays a significant role in the health and extent of management of native North American ash species in urban forests. An economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. Separate ash tree population valuations were derived from the i-Tree Streets program and the Council of Tree and Landscape Appraisers (CTLA) methodology. A relative economic analysis was used to compare a control option (do-nothing approach, only removing ash trees as they die) to three distinct management options: 1) preemptive removal of all ash trees over a 5 yr period, 2) preemptive removal of all ash trees and replacement with comparable nonash trees, or 3) treating the entire population of ash trees with insecticides to minimize mortality. For each valuation and management option, an annual analysis was performed for both the remaining ash tree population and those lost to emerald ash borer. Retention of ash trees using insecticide treatments typically retained greater urban forest value, followed by doing nothing (control), which was better than preemptive removal and replacement. Preemptive removal without tree replacement, which was the least expensive management option, also provided the lowest net urban forest value over the 20-yr simulation. A "no emerald ash borer" scenario was modeled to further serve as a benchmark for each management option and provide a level of economic justification for regulatory programs aimed at slowing the movement of emerald ash borer. PMID:22420272

  19. Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae

    Directory of Open Access Journals (Sweden)

    Elias Soares Gomes

    2014-06-01

    Full Text Available Biological aspects of Leucothyreus ambrosius Blanchard (Coleoptera, Melolonthidae, Rutelinae. Coleopterans of the family Melolonthidae comprise a large group of species that feed on different food sources, including plant roots, stems, and leaves, in addition to plant materials at different decomposition stages. Several species are found in the genus Leucothyreus, occurring in different regions of Brazil, including the various biomes in the country. Information on the biology of species of the genus Leucothyreus is scarce, therefore, we conducted studies on the biological aspects of Leucothyreus ambrosius Blanchard, 1850. The period of adult occurrence was determined with a light trap installed between a cropped and pasture area in the municipality of Aquidauana, Mato Grosso do Sul State, Brazil. Adults collected in the field were used to form insect pairs and the studies were initiated in the entomology laboratory as the adults began ovipositing. Adults were observed flying in the field from October to December. Eggs were obtained as pairs were formed and a colony was established, the embryonic period lasting 14.6 days on average. The larval period in the 1st instar lasted 21.6 days, in the 2nd instar 19.6 days, and in the 3rd instar, 85.6 days. The head capsule width was 1.48 mm in the 1st instar, 2.44 mm in the 2nd, and 3.83 mm in 3rd larval instar. The pupal stage had an average duration of 35.5 days. The egg to adult period lasted 173.3 days. Morphometric information for the larval and adult stages is presented in this study.

  20. Contributions to the knowledge of Atlantic Canadian Histeridae (Coleoptera

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    Christopher Majka

    2008-09-01

    Full Text Available New records of Histeridae from Atlantic Canada are reported. Three species are newly recorded from Prince Edward Island and two from New Brunswick, one of which, the introduced Palearctic Atholus bimaculatus (Linnaeus, is newly recorded from Atlantic Canada as a whole. These new records increase the known histerid fauna of the region to 37 species, 30 native and 7 introduced ones. The regional zoogeography of the Histeridae is examined focusing on differences between the faunal composition of the various provinces and the possible reasons responsible for these. The island faunas of Cape Breton, Prince Edward Island, and Newfoundland are examined. All have reduced faunas in comparison with the mainland perhaps as a result of island-associated diminutions, an area effect, a paucity of collecting, or a combination of these factors. Those of Cape Breton and Prince Edward Island are proportionately similar to those of other families of Coleoptera, whereas that of Newfoundland (only 10% of the mainland fauna is significantly less, a circumstance which deserves further investigation. Seven species of introduced histerids have been recorded in the region. The average dates of first detection of these species are much later than the earliest records of these species in North America and comparatively later than is the case with other suites of introduced species in the Staphylinidae and Carabidae, perhaps as a result of the sparse attention the Histeridae have historically received by coleopterists in the region. Most of the introduced histerids are known to be synanthropic and may have been introduced to the region association with the importation of livestock and materials related to animal husbandry. The Histeridae of the region largely fall into one of several trophic guilds: coastal species and those associated with beach-drift material; species associated with bird nests; species associated with mammal nests; myrmecophilus species; saproxylic

  1. Biocontrol of larval mosquitoes by Acilius sulcatus (Coleoptera: Dytiscidae

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    Banerjee Siddhartha S

    2008-10-01

    Full Text Available Abstract Background Problems associated with resistant mosquitoes and the effects on non-target species by chemicals, evoke a reason to find alternative methods to control mosquitoes, like the use of natural predators. In this regard, aquatic coleopterans have been explored less compared to other insect predators. In the present study, an evaluation of the role of the larvae of Acilius sulcatus Linnaeus 1758 (Coleoptera: Dytiscidae as predator of mosquito immatures was made in the laboratory. Its efficacy under field condition was also determined to emphasize its potential as bio-control agent of mosquitoes. Methods In the laboratory, the predation potential of the larvae of A. sulcatus was assessed using the larvae of Culex quinquefasciatus Say 1823 (Diptera: Culicidae as prey at varying predator and prey densities and available space. Under field conditions, the effectiveness of the larvae of A. sulcatus was evaluated through augmentative release in ten cemented tanks hosting immatures of different mosquito species at varying density. The dip density changes in the mosquito immatures were used as indicator for the effectiveness of A. sulcatus larvae. Results A single larva of A. sulcatus consumed on an average 34 IV instar larvae of Cx. quinquefasciatus in a 24 h period. It was observed that feeding rate of A. sulcatus did not differ between the light-on (6 a.m. – 6 p.m., and dark (6 p.m. – 6 a.m. phases, but decreased with the volume of water i.e., space availability. The prey consumption of the larvae of A. sulcatus differed significantly (P A. sulcatus larvae, while with the withdrawal, a significant increase (p A. sulcatus in regulating mosquito immatures. In the control tanks, mean larval density did not differ (p > 0.05 throughout the study period. Conclusion the larvae of the dytiscid beetle A. sulcatus proved to be an efficient predator of mosquito immatures and may be useful in biocontrol of medically important mosquitoes.

  2. Using malaise traps to sample ground beetles (Coleoptera: Carabidae).

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D., James L. Hanula, and Scott Horn

    2005-01-01

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  3. Using malaise traps to sample ground beetles (Coleoptera. Carabidae)

    Energy Technology Data Exchange (ETDEWEB)

    Ulyshen, Michael D. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Hanula, James L. [USDA Forest Service, Savannah River, New Ellenton, SC (United States); Horn, Scott [USDA Forest Service, Savannah River, New Ellenton, SC (United States)

    2012-04-02

    Pitfall traps provide an easy and inexpensive way to sample ground-dwelling arthropods (Spence and Niemela 1994; Spence et al. 1997; Abildsnes and Tommeras 2000) and have been used exclusively in many studies of the abundance and diversity of ground beetles (Coleoptera: Carabidae). Despite the popularity of this trapping technique, pitfall traps have many disadvantages. For example, they often fail to collect both small (Spence and Niemela 1994) and trap-shy species (Benest 1989), eventually deplete the local carabid population (Digweed et al. 1995), require a species to be ground-dwelling in order to be captured (Liebherr and Mahar 1979), and produce different results depending on trap diameter and material, type of preservative used, and trap placement (Greenslade 1964; Luff 1975; Work et al. 2002). Further complications arise from seasonal patterns of movement among the beetles themselves (Maelfait and Desender 1990), as well as numerous climatic factors, differences in plant cover, and variable surface conditions (Adis 1979). Because of these limitations, pitfall trap data give an incomplete picture of the carabid community and should be interpreted carefully. Additional methods, such as use of Berlese funnels and litter washing (Spence and Niemela 1994), collection from lights (Usis and MacLean 1998), and deployment of flight intercept devices (Liebherr and Mahar 1979; Paarmann and Stork 1987), should be incorporated in surveys to better ascertain the species composition and relative numbers of ground beetles. Flight intercept devices, like pitfall traps, have the advantage of being easy to use and replicate, but their value to carabid surveys is largely unknown. Here we demonstrate the effectiveness of Malaise traps for sampling ground beetles in a bottomland hardwood forest.

  4. Short-term effects of dimethoate on metabolic responses in Chrysolina pardalina (Chrysomelidae) feeding on Berkheya coddii (Asteraceae), a hyper-accumulator of nickel

    International Nuclear Information System (INIS)

    Berkheya coddii Roessler (Asteraceae) is a hyper-accumulator of nickel, which can be used in phytomining and phytoremediation. Chrysolina pardalina Fabricius (Chrysomelidae) is a phytophagous leaf beetle, which may be useful in controlling population levels of B. coddii after it has been introduced into a new habitat. The aim of this study was to investigate the response of C. pardalina to topical application of dimethoate. Data recorded included the activity of acetylcholinesterase (AChE), the concentration of glutathione (GSH), and the activity of selected enzymes connected with GSH metabolism. Assays were carried out several times during the first 24 h after exposure to dimethoate. At the dosages used in this study, dimethoate was not as toxic as expected. AChE activity was significantly decreased 14 and 24 h after application. GST activity was significantly decreased 24 h after application. GSTPx activity was significantly decreased 2, 14 and 24 h after application. GR activity was significantly increased 4 h after application. GSH concentration was significantly increased 24 h after application. Long-term exposure to high levels of nickel may have caused adaptive changes in the enzymes that enable C. pardalina to deal with other stressors, including organophosphate pesticides. - Long-term exposure to high levels of nickel may have caused adaptive changes in the enzymes that enable Chrysolina pardalina to deal with other stressors, including organophosphate pesticides

  5. Digestion of Starch Granules from Maize, Potato and Wheat by Larvae of the the Yellow Mealworm, Tenebrio molitor and the Mexican Bean Weevil, Zabrotes subfasciatus

    OpenAIRE

    Meireles, Elaine A.; Carneiro, Cíntia N. B.; Renato A DaMatta; Samuels, Richard I; Silva, Carlos P

    2009-01-01

    Scanning electron microscopy images were taken of starch granules from different sources following exposure in vivo and in vitro to gut α-amylases isolated from Tenebrio molitor L. (Coleoptera: Tenebrionidae) and Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae). One α-amylase was isolated from whole larval midguts of T. molitor using non-denaturing SDS-PAGE, while two other α-amylase fractions were isolated from whole larval midguts of Z. subfasciatus using hydrophobic interaction chroma...

  6. Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Dilma Solange Napp

    2006-01-01

    Full Text Available Novos táxons sul-americanos de Compsocerini (Coleoptera, Cerambycidae. Um novo gênero e quatro novas espécies, todos provenientes da Bolívia, são descritos em Compsocerini: Ecoporanga gen. nov., espécie-tipo E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., procedentes de Santa Cruz e Ethemon iuba sp. nov. proveniente de Cochabamba.New South American taxa of Compsocerini (Coleoptera, Cerambycidae. The following new genus and species, all from Bolivia, are described in Compsocerini: Ecoporanga gen. nov., type species E. achira sp. nov., Dilocerus brunneus sp. nov., Rierguscha florida sp. nov., from Santa Cruz, and Ethemon iuba sp. nov. from Cochabamba.

  7. Una especie nueva de Trechisibus de la Argentina (Coleoptera: Carabidae A new species of Trechisibus from Argentina (Coleoptera: Carabidae

    Directory of Open Access Journals (Sweden)

    Sergio Roig-Juñent

    2005-12-01

    Full Text Available RESUMEN. Las Trechini constituyen una tribu de Carabidae distribuida ampliamente en la región Andino Patagónica. A pesar de que existen varias revisiones parciales de esta tribu para la Argentina y Chile, es muy común hallar especies nuevas, debido sobre todo a la prospección de áreas no exploradas. En este aporte se describe una especie nueva del género Trechisibus Jeannel del Cerro Nevado (Mendoza, Argentina. Por sus características morfológicas pertenece al grupo de especies depressus. Se describe e ilustra el adulto de esta especie nueva, se provee una clave para la identificación de las especies del grupo depressus y se discuten algunos aspectos de la distribución de estas especies.ABSTRACT. Trechini is a tribe of Carabidae (Coleoptera widely distributed in the Andean Patagonian region in South America. In spite of several partial revisions of the tribe for Argentina and Chile, it is very common to find new species due to the research of unexplored areas. In the present paper, a new species of the genus Trechisibus Jeannel from the Cerro Nevado (Mendoza, Argentina is described. Based on its morphological features the new species is considered as a member of the depressus group. The adult of the new species is described and illustrated, a key for the identification of the species of the depressus group is provided, and some aspects of the distribution of the group are discussed.

  8. Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil

    OpenAIRE

    Renata C. Campos; Malva I. Medina Hernández

    2013-01-01

    Dung beetle assemblages (Coleoptera, Scarabaeinae) in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung) or carcasses (carrion) and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the ...

  9. Species of beetles (Coleoptera; Scarabaeidae associated to banana (Musa spp. in Ceballos, Ciego de Avila, Cuba

    Directory of Open Access Journals (Sweden)

    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in bananas plantations, according to Sisne, 2009 and MINAG, 1985, in the Citric enterprise of Ciego de Ávila during the period between May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species Cyclocephala cubana Chapin, Phyllophaga puberula Duval, and Phyllophaga patruelis Chev. are associated to bananas crops in these areas.

  10. Ecological impact of entomopathogenic nematodes used to control the large pine weevil, Hylobius abietis (Coleoptera: Curculionidae)

    OpenAIRE

    Harvey, Christopher D.

    2010-01-01

    The large pine weevil (Hylobius abietis; Coleoptera: Curculionidae) is one of the most economically damaging pests in forestry across Northern Europe. Its larvae develop in coniferous tree stumps on clearfell sites and adult weevils feed on seedlings that are replanted on these sites, causing substantial mortality. As the drive towards achieving the sustainable management of natural resources increases, biological control agents are being considered as an alternative to chemical ...

  11. Otiorhynchus spp. (Coleoptera: Curculionidae) as pests in horticulture: genetics and management options with entomopathogenic fungi

    OpenAIRE

    Hirsch, Jacqueline

    2012-01-01

    Worldwide, weevils of the genus Otiorhynchus (Coleoptera: Curculionidae) cause damage with detrimental economic effects to many horticultural crops due to the root feeding of their larvae as well as foliage feeding of their adults. Aside from the black vine weevil Otiorhynchus sulcatus, which is the best-known pest within this genus, numerous other Otiorhynchus species have been increasingly recognized as pests in recent years. Nocturnal adult weevils and soil-inhibiting larvae are in princip...

  12. Laboratory Rearing of Laricobius nigrinus (Coleoptera: Derodontidae): A Predator of the Hemlock Woolly Adelgid (Hemiptera: Adelgidae)

    OpenAIRE

    Salom, S. M.; L. T. Kok; Lamb, A B; Jubb, C

    2012-01-01

    Coleopteran species are biological control agents of numerous invasive pests. Laricobius nigrinus (Coleoptera: Derodontidae), a predaceous, univoltine species, spends the summer aestivating but is active for the rest of the year. Laricobius nigrinus possesses many essential attributes for effective biological control of the hemlock woolly adelgid (Hemiptera: Adelgidae). The predator must be reared in large numbers for field releases. We describe some of the studies that led to the successful ...

  13. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae)

    OpenAIRE

    Andréa Roveré Franz; Neiva Knaak; Lidia Mariana Fiuza

    2011-01-01

    Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae). Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf), Zingiber officinale (root) and Mentha sp. (leaf). The efficacy of ...

  14. New species and records of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Bolivia.

    Science.gov (United States)

    Arce-Pérez, Roberto; Morón, Miguel Ángel

    2014-01-01

    Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Bolivia are described and illustrated: M. megaphyllus new species (from Comarapa, Santa Cruz and Sehuenca, Cochabamba) and M. yunganus new species (from Mairana and Comarapa, Santa Cruz). In addition, the species Macrodactylus bolivianus Moser, M. gracilis Moser, and M. nobilis Frey are redescribed and illustrated to help facilitate identification of these species. A key to the 10 species of Macrodactylus presently known from Bolivia is provided.  PMID:25283110

  15. Wireworms’ Management: An Overview of the Existing Methods, with Particular Regards to Agriotes spp. (Coleoptera: Elateridae

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    Fanny Barsics

    2013-01-01

    Full Text Available Wireworms (Coleoptera: Elateridae are important soil dwelling pests worldwide causing yield losses in many crops. The progressive restrictions in the matter of efficient synthetic chemicals for health and environmental care brought out the need for alternative management techniques. This paper summarizes the main potential tools that have been studied up to now and that could be applied together in integrated pest management systems and suggests guidelines for future research.

  16. Lectotype designations and nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae)

    OpenAIRE

    Sandoval-Gómez,Vivian E.; Cristiano Lopes-Andrade; John Lawrence

    2014-01-01

    We designate lectotypes and propose nomenclatural changes in Xylographus Mellié (Coleoptera, Ciidae) based on type specimens deposited in the Museum of Comparative Zoology (USA), Museum für Naturkunde Berlin (Germany), the Natural History Museum (UK), Muséum d’Histoire Naturelle de la Ville de Genève (Switzerland), Muséum National d’Histoire Naturelle (France), Naturhistoriska Riksmuseet (Sweden) and Naturhistorisches Museum Wien (Austria). We desi...

  17. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern

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    SUGIYARTO

    2009-07-01

    Full Text Available Maryati KT, Sugiyarto. 2010. Characterization of white grub (Melolonthidae; Coleoptera in salak plantation based on morphology and protein banding pattern. Nusantara Bioscience 1: 72-77. This research aims to find out the white grub (Melolonthidae; Coleoptera variability based on the morphological characteristic and protein banding pattern found in ”salak pondoh” farm in Regencies of Sleman, Yogyakarta and Magelang, Central Java. Each area has five sampling points. Morphological analysis on white grub was conducted using descriptive method and analysis on protein banding pattern was conducted using qualitative analysis based on the presence or absent of band pattern on the gel, and qualitatively based on the relative mobility value (Rf of protein. The result indicated that the white grub in Sleman and Magelang, based on morphology characteristic is only one species, namely Holothricia sp. Based on the protein banding pattern, the white grub sample have differences of protein band number and protein molecular weight. Key words: Salacca zalacca, white grub, morphology, protein banding pattern.Abstrak. Maryati KT, Sugiyarto. 2010. Karakterisasi lundi putih (Melolonthidae: Coleoptera pada pertanaman salak berdasarkan ciri morfologi dan pola pita protein. Nusantara Bioscience 1: 72-77. Penelitian ini bertujuan untuk mengetahui keanekaragaman lundi putih (Melolonthidae; Coleoptera berdasarkan ciri morfologi dan pola pita protein yang ditemukan di lahan pertanaman salak pondoh di Kabupaten Sleman, Yogyakarta dan Kabupaten Magelang, Jawa Tengah. Pada masing-masing wilayah diambil lima titik sampling. Analisis morfologi lundi putih digunakan metode deskriptif, dan analisis pola pita protein digunakan analisis kualitatif berdasarkan muncul tidaknya pola pita pada gel, dan secara kuantitatif berdasarkan nilai mobilitas relatif protein (RF. Hasil penelitian menunjukkan bahwa sampel lundi putih di Kabupaten Sleman dan Magelang, berdasar karakter

  18. A new genus and four new species of false click beetles (Coleoptera: Eucnemidae) from Southeast Asia

    OpenAIRE

    Otto, Robert L.

    2016-01-01

    A new genus and four new species of false click beetle (Coleoptera: Eucnemidae) are described. Bermilloides new genus is described from Bornean Malaysia. New species are: Calyptocerus iridis new species (Philippines), Bermilloides lumawigi new species (Malaysia), Spinifornax elongatus new species (Malaysia) and Ceratus antennatus new species (Thailand). Dorsal and ventral habitus, as possible, for each species are illustrated. Male aedeagi are illustrated for Calyptocerus iridis and Ceratu...

  19. Life History of the Tamarind Weevil, Sitophilus linearis (Herbst) (Coleoptera: Curculionidae), on Tamarind Seed

    OpenAIRE

    James Adebayo Ojo; Adebayo Amos Omoloye

    2015-01-01

    The tamarind weevil, Sitophilus linearis Herbst (Coleoptera: Curculionidae), is an important pest of tamarind and other Caesalpinioideae. Investigating its life history is important in the implementation of management strategy. Its life history was monitored daily to understand its developmental biology on tamarind seed following standard procedures under laboratory conditions of 24–30°C temperature, 60–70% relative humidity, and 12L : 12D photoperiod. The egg incubation period lasted 3.17 ± ...

  20. Studies on the Dung-inhabiting Beetles (Insecta: Coleoptera Community of Western Anatolia, Turkey

    Directory of Open Access Journals (Sweden)

    Sinan Anlaş

    2011-12-01

    Full Text Available In Bozdağlar Mountain of western Turkey, the diversity and composition of the dung-inhabiting beetles in two locations situated in different altitudes (600 m and 900 m in 2004 and 2006 assemblages were sampled. A total of 5.709 individuals from 88 species belonging to the families Scarabaeidae, Aphodiidae, Geotrupidae, Carabidae, Hydrophilidae, Histeridae and Ptilidae of the order Coleoptera are recorded.

  1. Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil

    OpenAIRE

    Andreia Mauruto Chernaki-Leffer; Daniel Ricardo Sosa-Gómez; Almeida, Lúcia M.; Ivani de Oliveira Negrão Lopes

    2011-01-01

    Susceptibility of Alphitobius diaperinus (Panzer) (Coleoptera, Tenebrionidae) to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer), is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvo...

  2. Novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae da Bolívia

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    Ubirajara R. Martins

    2008-01-01

    Full Text Available Novas espécies descritas da Bolívia, Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.New species of Onciderini (Coleoptera, Cerambycidae, Lamiinae from Bolivia. New species described from Santa Cruz: Trachysomus apipunga sp. nov., Hesychotypa aotinga sp. nov., Cacostola apyraiuba sp. nov. and Glypthaga nearnsi sp. nov.

  3. Penggunaan Beberapa Serbuk Rimpang Terhadap Mortalitas Hama Callosobruchus chinensis L. (Coleoptera; Bruchidae) Pada Kacang Hijau

    OpenAIRE

    Hayati, Anni

    2012-01-01

    Anni Hayati, "Use of Some Powdered Rhizome Mortality Against Pests Callosobruchus chinensis L. (Coleoptera: Bruchidae) On the Green Bean ". It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. C.chinensis L. are pests on green beans in storage which causes considerable yield loss, one of effective control is the use of botanical insecticides. Use of some powdered rhizome on mortality C.chinensis L. in a green bean plant pests carried in the laboratory of t...

  4. Ernährungsphysiologische Aspekte xylophager Bockkäferlarven (Coleoptera: Cerambycidae)

    OpenAIRE

    Grünwald, Sebastian

    2009-01-01

    Mittels Fluoreszenz-in-situ-Hybridisierung und PCR-basierter rRNA-Sequenzierung wurde die Darm- und Myzetomflora xylophager Insektenlarven (Coleoptera: Cerambycidae) untersucht. Die Myzetome enthalten hefeartige Symbionten in Form von Candida rhagii und Candida shehatae. Das Darmlumen der Tiere beherbergt eine hoch variable Gemeinschaft von Bakterien und Pilzen, die zum Teil der Nahrung entstammt. Die Larven des Hausbockkäfers nehmen eine Sonderstellung ein: Ihnen fehlen sowohl Myzetome als a...

  5. Defensive Glands of the Darkling Beetle Mesomorphus villiger Blanchard (Coleoptera: Tenebrionidae)

    OpenAIRE

    Seena, C. M.; Sabu K. Thomas

    2013-01-01

    Massive home invasion by the darkling beetle Mesomorphus villiger Blanchard 1853 (Coleoptera: Tenebrionidae) during monsoon season make it a nuisance pest in many regions of south India. Morphology of defensive glands and mode of release and dispersal of the defensive secretion were analysed. Defensive glands were separated from the abdominal sternites by cutting along the posterior margin of the seventh sternite. Glands are evaginations of intersegmental membrane between the seventh and eigh...

  6. DIVERSITY OF CARABIDS (COLEOPTERA) ASSOCIATED WITH THE LOWER LURIN RIVER, LIMA, PERU

    OpenAIRE

    ARMANDO VÉLEZ-AZAÑERO; Alfonso Lizárraga-Travaglini

    2013-01-01

    Quarterly samples of beetles (Coleoptera: Carabidae) were taken associated with the lower basin of the Lurín River (Lima-Peru) during the period August 2009 - February 2011, in six sampling points between 5 and 51 masl. Pitfall traps were used and obtained a total of 59 specimens distributed among three tribes, three genera, and four morphospecies. We report the presence of Megacephala, Scarites genus, and Pterostichus with the latter being the predominant genus.

  7. New national and state records of Neotropical Staphylinidae (Insecta: Coleoptera).

    Science.gov (United States)

    Asiain, Julieta; Márquez, Juan; Irmler, Urlich

    2015-01-01

    Eighteen new national records of nine species of Osoriinae (Staphylinidae) are added for 10 Neotropical countries. Additionally, 17 species of three subfamilies are first recorded from ten States of México. The distributional patterns of the studied species are commented and the congruence with species of different families of Coleoptera and Odonata previously analyzed is discussed. Finally, we conclude that some of these patterns can be proposed as hypothesis of primary biogeographic homology. PMID:26249885

  8. Incorporating a Sorghum Habitat for Enhancing Lady Beetles (Coleoptera: Coccinellidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    Lady beetles (Coleoptera: Coccinellidae) prey on insect pests in cotton. The objective of this 2 yr on-farm study was to document the impact of a grain sorghum trap crop on the density of Coccinellidae on nearby cotton. Scymnus spp., Coccinella septempunctata (L.), Hippodamia convergens Guérin-Méneville, Harmonia axyridis (Pallas), Coleomegilla maculata (De Geer), Cycloneda munda (Say), and Olla v-nigrum (Mulsant) were found in sorghum over both years. Lady beetle compositions in sorghum and ...

  9. A checklist of the genus Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae of the world

    Directory of Open Access Journals (Sweden)

    G. Mahendiran

    2013-08-01

    Full Text Available An annotated checklist of Blosyrus Schoenherr (Coleoptera: Curculionidae: Entiminae comprising 101 species with their updated nomenclature, synonyms and distribution is given. The distribution pattern indicates that the genus is diversified mostly in the Sub-Saharan Africa and Asia regions. Out of 101 species, 92 occur in Sub-Saharan Africa. From India, eight species were recorded. In India, the distribution is mainly in West Bengal, Assam, Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu.

  10. Biological activities of Allium sativum essential oil against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae)

    OpenAIRE

    Chaubey Mukesh Kumar

    2014-01-01

    Essential oil from Allium sativum was isolated and investigated for its repellent, insecticidal, ovipositional and egg hatching inhibition activities against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). A. sativum essential oil repelled bruchid adults at a very low concentration in choice oviposition assay. A. sativum essential oil caused both fumigant and contact toxicity in C. chinensis adults in a concentration dependent manner. Oviposition potency of C. chinensis adults...

  11. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

    OpenAIRE

    M.M. Kiradoo; Meera Srivastava

    2011-01-01

    The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae) is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insect...

  12. Three Boll Weevil Diapause Myths in Perspective

    Science.gov (United States)

    The boll weevil, Anthonmus grandis grandis Boheman (Coleoptera: Curculionidae), originated in Mesoamerica but its contemporary distribution extends from the United States Cotton Belt to Argentina, throughout which it is a serious pest of cotton, Gossypium hirsutum L. While research on the boll weev...

  13. Integrated Pest Management for sweetpotato in Eastern Africa.

    NARCIS (Netherlands)

    Smit, N.E.J.M.

    1997-01-01

    Sweetpotato is an important crop in Eastern Africa. Sweetpotato weevils ( Cylas puncticollis Boheman and C. brunneus Fabricius; Coleoptera: Apionidae) cause damage to roots and vinesthroughout the crop's production area. Other insect pests of sweetpotato are of regional importance. The

  14. Potential for transport of boll weevils (Coleoptera: Curculionidae) to the cotton gin within cotton modules.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Norman, John W; Knutson, Allen E

    2004-06-01

    There is concern that cotton gins located in boll weevil, Anthonomus grandis grandis Boheman, eradication zones serving customers in adjacent infested zones may serve as a site for boll weevil reintroductions if weevils are transported alive inside cotton modules. We surveyed fields in three distinct areas of Texas and found that weevils can be present in large numbers in cotton fields that have been defoliated and desiccated in preparation for harvest, both as free adults and as immatures inside unopened bolls. Harvested cotton taken from module builders indicated that approximately = 100-3700 adult boll weevils were packed inside modules constructed at the sampled fields. Marked weevils were forced through a laboratory field cleaner (bur extractor) commonly mounted on stripper-harvesters, and 14% were recovered alive in the seed cotton fraction and lived at least to 24 h. Survival of weevils placed inside modules declined over time up to 7 d, but the magnitude of the decline varied with experimental conditions. In one experiment, 91% of the weevils survived to 7 d, whereas under harsher environmental conditions, only 11% survived that long. Together, our results indicate that when cotton is harvested in an infested area, boll weevils likely will be packed alive into cotton modules, and many will still be alive by the time the module is fed into the gin, at least up to 7 d after the module's construction. PMID:15279274

  15. Boll weevil (Coleoptera: Curculionidae) survival through the seed cotton cleaning process in the cotton gin.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-08-01

    There is concern that gins located in boll weevil, Anthonomus grandis grandis Boheman, eradication zones may become points of reintroduction when they process cotton grown in a neighboring infested area. We estimated boll weevil survival through two typical machine sequences used in commercial cotton gins to clean and dry the seed cotton in advance of the gin stand, as well as separately through two incline cylinder cleaners or one or two tower dryers operating at different temperatures. Large numbers of laboratory-reared adult boll weevils were marked with fluorescent powder, fed into the test system, and recovered with the assistance of blacklights. We found no evidence of survival through the seed cotton cleaning systems even when the dryers were not heated, or when passed separately through the two incline cleaners alone. Upper confidence limits (95%) were calculated for the observed zero recoveries based on sample size and the binomial distribution, and these represent the statistical worst-case (i.e., highest) survival potential. Survival through heated tower dryers declined rapidly to zero at higher temperatures, especially when two dryers were running. Although we conclude that the potential for survival of weevils in the seed cotton to the gin stand is zero or close to zero, a small percentage of live weevils was recovered in the green boll/rock trap, which may represent the greatest threat of reintroduction at the gin. Escape of live weevils with the gin trash is also possible, and studies addressing this issue will be presented elsewhere. PMID:15384344

  16. Behavioral responses of plum curculio (Coleoptera: Curculionidae) to different enantiomer concentrations and blends of the synthetic aggregation pheromone grandisoic acid

    Science.gov (United States)

    Host plant odors are important for insect location of food and mates. Synergy between host plant odors and aggregation pheromones occurs in many Curculionidae species. The plum curculio Conotrachelus nenuphar Herbst (Coleoptera: Curculionidae) is a major pest of pome and stone fruit. Males produce t...

  17. From forest to plantation? Obscure papers reveal alternate host plants for the coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae)

    Science.gov (United States)

    The coffee berry borer, Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae), is the most devastating insect pest of coffee throughout the world. The insect is endemic to Africa but can now be found throughout nearly all coffee producing countries. One area of the basic biology of the insec...

  18. First record of the agave snout weevil, Scyphophorus acupunctatusGyllenhal (Coleoptera: Curculionidae: Dryophthorinae), in Puerto Rico

    OpenAIRE

    Setliff, Gregory P.; Anderson, Jesse A.

    2011-01-01

    The agave snout weevil, Scyphophorus acupunctatus Gyllenhal (Coleoptera: Curculionidae: Dryophthorinae), is reported from Puerto Rico for the first time. It was collected on feral sisal, Agave sisalana Perrine (Agavaceae), in the Guánica Dry Forest Reserve in the southwestern part of the island.

  19. Redescription of the Hispaniolan ladybird genus Bura Mulsant (Coleoptera: Coccinellidae) and justification for its transfer from Coccidulinae to Sticholotidinae

    Science.gov (United States)

    In the current work, we discuss the features of Bura (Coleoptera: Coccinellidae) that justify its transfer from Coccidulinae to Sticholotidinae, speculate on circumstances that led to its prior misclassification, and highlight current problems in the delineation of the afforementioned lady beetle su...

  20. A new genus for Drepanocanthoides larreae (Horn, 1887) and description of a new congeneric Mexican species (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Marco DELLACASA; Dellacasa, Giovanni; Gordon, Robert D.

    2016-01-01

    Drepanocanthoides larreae (Horn, 1887) (Coleoptera: Scarabaeidae: Aphodiinae) is transferred to the new genus Hornosus and the new species Hornosus turnbowi from Mexico (Nuevo León) is described. A complete set of fi gures is supplied for the taxa dealt with herein.

  1. Saprovisca sarangay, new species, a second species in an unusual Philippine genus of Eupariini (Coleoptera: Scarabaeidae: Aphodiinae)

    OpenAIRE

    Skelley, Paul E.

    2016-01-01

    A new species of Saprovisca Stebnicka (Coleoptera: Scarabaeidae) is described from the Philippines. This new species possesses sexual dimorphism in the form of clypeal horns in males which also show major and minor allometry, both are conditions rare in the Aphodiinae.

  2. Agrilus rubensteini, a new species from the Philippines related to the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae)

    Science.gov (United States)

    A new species from the Philippines closely related to the emerald ash borer, Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae) is described: Agrilus rubensteini Chamorro & Jendek, new species. This is the first species in the A. cyaneoniger species-group recorded for the Philippines. Agr...

  3. Upper lethal temperature limits of the common furniture beetle Anobium punctatum (Coleoptera: Anobiidae)

    DEFF Research Database (Denmark)

    Hansen, Lise Stengård; Jensen, Karl-Martin Vagn

    The susceptibility of the egg, larval and adult stages of Anobium punctatum De Geer (Coleoptera: Anobiidae) to heat (46-54°C, 25-30% RH) was investigated. The larval stage was found to be most tolerant to heat. Very short exposure (5 min) of the larvae to temperatures of 52°C and above led to 100...

  4. Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Ontario

    Science.gov (United States)

    Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication program. The plan consisted of removing all infested trees and all trees within 400 m of an infested tree belonging to a genus consider...

  5. Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee

    Science.gov (United States)

    Metallic wood boring insects (Coleoptera: Buprestidae) species are responsible for high levels of host plant injury to deciduous shade and flowering trees in commercial nurseries, urban forests, and managed landscapes. Ornamental plant producers in the southeastern U.S. have ranked borers, includin...

  6. Resistance in Cultivated Sunflower Germplasm to the Red Sunflower Seed Weevil (Coleoptera: Curculionidae) in the Northern Great Plains

    Science.gov (United States)

    A 6-year field study evaluated 52 sunflower, Helianthus annuus L., accessions, 20 breeding lines, and 9 interspecific crosses for resistance to infestation by naturally occurring populations of the red sunflower seed weevil, Smicronyx fulvus LeConte (Coleoptera: Curculionidae). Germplasm with potent...

  7. Introduction and Recovery of Delphastus catalinae (Coleoptera: Coccinellidae) as a predator of Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt

    Science.gov (United States)

    Bemisia tabaci (Gennadius) (Hemiptera: Alyerodidae) is an important pest of many crops on a global scale. The use of biological control organisms such as coccinelid predators can help manage this pest. Delphastus catalinae (Horn) (Coleoptera: Coccinelidae) is an obligate predator of whiteflies, in...

  8. Quantitative relationship between potato tuber damage and counts of Pacific Coast wireworm (Coleoptera: Elateridae) in baits: seasonal effects.

    Science.gov (United States)

    Plots were baited with rolled oats in spring to assess the relationship between counts of Pacific coast wireworm, Limonius canus (Coleoptera: Elateridae) and damage to potato tubers. Baiting was done at 7 intervals beginning before planting of potatoes and ending following germination. Injury (per...

  9. Self-selection of two diet components by Tennebrio molitor (Coleoptera: Tenebrionidae) larvae and its impact on fitness

    Science.gov (United States)

    We studied the ability of Tenebrio molitor L. (Coleoptera: Tenebrionidae) to self-select optimal ratios of two dietary components to approach nutritional balance and maximum fitness. Life table analysis was used to determine the fitness of T. molitor developing in diet mixtures comprised of four dif...

  10. A model for long-distance dispersal of boll weevils (Coleoptera: Curculionidae)

    Science.gov (United States)

    Westbrook, John K.; Eyster, Ritchie S.; Allen, Charles T.

    2011-07-01

    The boll weevil, Anthonomus grandis (Boheman), has been a major insect pest of cotton production in the US, accounting for yield losses and control costs on the order of several billion US dollars since the introduction of the pest in 1892. Boll weevil eradication programs have eliminated reproducing populations in nearly 94%, and progressed toward eradication within the remaining 6%, of cotton production areas. However, the ability of weevils to disperse and reinfest eradicated zones threatens to undermine the previous investment toward eradication of this pest. In this study, the HYSPLIT atmospheric dispersion model was used to simulate daily wind-aided dispersal of weevils from the Lower Rio Grande Valley (LRGV) of southern Texas and northeastern Mexico. Simulated weevil dispersal was compared with weekly capture of weevils in pheromone traps along highway trap lines between the LRGV and the South Texas / Winter Garden zone of the Texas Boll Weevil Eradication Program. A logistic regression model was fit to the probability of capturing at least one weevil in individual pheromone traps relative to specific values of simulated weevil dispersal, which resulted in 60.4% concordance, 21.3% discordance, and 18.3% ties in estimating captures and non-captures. During the first full year of active eradication with widespread insecticide applications in 2006, the dispersal model accurately estimated 71.8%, erroneously estimated 12.5%, and tied 15.7% of capture and non-capture events. Model simulations provide a temporal risk assessment over large areas of weevil reinfestation resulting from dispersal by prevailing winds. Eradication program managers can use the model risk assessment information to effectively schedule and target enhanced trapping, crop scouting, and insecticide applications.

  11. Captures of Boll Weevils (Coleoptera: Curculionidae) in Relation to Trap Orientation and Distance From Brush Lines.

    Science.gov (United States)

    Spurgeon, Dale W

    2016-04-01

    Eradication programs for the boll weevil (Anthonomus grandis grandis Boheman) rely on pheromone-baited traps to trigger insecticide treatments and monitor program progress. A key objective of monitoring in these programs is the timely detection of incipient weevil populations to limit or prevent re-infestation. Therefore, improvements in the effectiveness of trapping would enhance efforts to achieve and maintain eradication. Association of pheromone traps with woodlots and other prominent vegetation are reported to increase captures of weevils, but the spatial scale over which this effect occurs is unknown. The influences of trap distance (0, 10, and 20 m) and orientation (leeward or windward) to brush lines on boll weevil captures were examined during three noncropping seasons (October to February) in the Rio Grande Valley of Texas. Differences in numbers of captured weevils and in the probability of capture between traps at 10 or 20 m from brush, although often statistically significant, were generally small and variable. Variations in boll weevil population levels, wind directions, and wind speeds apparently contributed to this variability. In contrast, traps closely associated with brush (0 m) generally captured larger numbers of weevils, and offered a higher probability of weevil capture compared with traps away from brush. These increases in the probability of weevil capture were as high as 30%. Such increases in the ability of traps to detect low-level boll weevil populations indicate trap placement with respect to prominent vegetation is an important consideration in maximizing the effectiveness of trap-based monitoring for the boll weevil. PMID:26719592

  12. Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Marcela L. Monné

    2012-09-01

    Full Text Available Novos táxons em Acanthocinini sul-americanos (Coleoptera, Cerambycidae. São descritos e figurados os novos táxons: Trichonyssodrys nessimiani sp. nov. do Brasil (Bahia a Santa Catarina, Amniscites tavakiliani sp. nov. da Guiana Francesa and A. amboroensis sp. nov. da Bolívia (Cochabamba e Santa Cruz; Trichonius inusitatus sp. nov. e Lophopoenopsis itatiaiensis sp. nov. do Brasil (Rio de Janeiro e Luteolepturges gen. nov., espécie tipo L. galbus sp. nov., do Brasil (Amazonas, Rondônia, Peru e Equador.

  13. Nothoprodontia, um novo gênero de Trachyderini (Coleoptera, Cerambycidae, Cerambycinae

    Directory of Open Access Journals (Sweden)

    Miguel A. Monné

    2004-06-01

    Full Text Available Nothoprodontia gen. nov. e sua espécie-tipo, N. boliviana sp. nov., são descritos da Bolívia (Cochabamba. O novo gênero é comparado com Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870.Nothoprodontia, a new genus of Trachyderini (Coleoptera, Cerambycidae, Cerambycinae. Nothoprodontia gen. nov. and its type-species, N. boliviana sp. nov., are described from Bolívia (Cochabamba. Comparative notes with Prodontia Audinet-Serville, 1834, Eriphus Audinet-Serville, 1834, and Athetesis Bates, 1870 are also given.

  14. Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico

    Directory of Open Access Journals (Sweden)

    Sonia Maria Noemberg Lazzari

    2006-06-01

    Full Text Available Resfriamento artificial para o controle de Coleoptera em arroz armazenado em silo metálico. O objetivo desta pesquisa foi avaliar o efeito do resfriamento artificial de grãos de arroz para o controle de coleópteros-praga. O ar frio foi insuflado pelo sistema de aeração em um silo metálico com arroz-em-casca. A avaliação do tratamento foi feita quinzenalmente usando armadilhas caladores. As espécies de Coleoptera capturadas foram: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16,5% e Sitophilus spp. (0,5%. Aos 28 dias, a temperatura média da massa de grãos era de 15ºC, e o número médio de insetos havia diminuído 76,8%. A aplicação de ar frio manteve as populações sob controle por aproximadamente 60 dias. Os resultados do monitoramento dos insetos e da temperatura indicaram que um novo ciclo de ar frio deveria ser aplicado nesse período para manter as populações sob controle. Também o manejo adequado da massa de grãos faz-se necessário para garantir resultados satisfatórios do resfriamento artificial.Artificial chilling to control Coleoptera in paddy rice stored in metallic silo. The objective of this research was to evaluate the effect of artificial chilling for the control of coleopterans in stored paddy rice. The cold air was insufflated through the aeration system of a metallic silo with paddy rice. Evaluation of insect number was made every 15 days using probe traps. The species of Coleoptera captured were: Oryzaephilus surinamensis (60%; Cryptolestes ferrugineus (9%; Rhyzopertha dominica (16.5% and Sitophilus spp. (0.5%. By the 28th day the average temperature of the grain mass was 15ºC, and the mean number of insects decreased 76.8%. The cold air application kept the insect populations under control for approximately 60 days. The results of temperature and insect monitoring indicated that a new cycle of cold air should be applied by that time to keep the populations under

  15. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-09-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  16. Bioluminescence emissions of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae)

    Indian Academy of Sciences (India)

    A Gohain Barua; S Hazarika; N M Saikia; G D Baruah

    2009-06-01

    We recorded the in vivo emission and time-resolved spectra of the firefly Luciola praeusta Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae). The emission spectrum shows that the full width at half maximum (FWHM) value for this particular species is 55 nm, which is significantly narrower than the in vivo half-widths reported till now. The time-resolved spectrum reveals that a flash of about 100 ms duration is, in fact, composed of a number of microsecond pulses. This suggests that the speed of the enzyme-catalysed chemiluminescence reaction in the firefly for the emission of light is much faster than was previously believed.

  17. Invertebrate fauna (Coleoptera, Collembola, Diplopoda, Isopoda collected in the karst areas of the Aninei - Locvei Mountains

    Directory of Open Access Journals (Sweden)

    Andrei Giurginca

    2011-08-01

    Full Text Available The authors identified 132 species of invertebrates (14 Oniscidea, 25 Diplopoda,31 Collembola and 62 Coleoptera recently sampled (2001–2006 from the soil and subterranean (MSS and caves environments from the Banat Mountains. Some new,rare and endemic species are discussed. The seasonal changes of the species diversity in the superficial subterranean environments at 0.5 to 1 m in depth are for the first time presented for the Reşiţa – Moldova Nouă synclinorium. The characteristic and preferential species for the mesovoid shallow substratum (MSS, belonging to the analyzed taxa, are identified.

  18. A review of the natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries.

    Science.gov (United States)

    Di Iorio, Osvaldo

    2014-01-01

    A compilation of the known natural history of adult Cetoniinae (Coleoptera: Scarabaeidae) from Argentina and adjacent countries is provided. Food items of adult Cetoniinae include pollen and/or nectar (flower visitors), sap and/or slime flux, ripened fruits on plants, green tissues and leaves, and honey. Of the 36 species of Cetoniinae from Argentina, food items are known only for 11 species (30.5%). Attraction to light and bait-traps, adult activity periods, vertebrate predators, and the occurrence in bird nests are presented and discussed. Other insects that share the same food sources and bait-traps with Cetoniinae are mentioned. PMID:24869870

  19. Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico

    Directory of Open Access Journals (Sweden)

    Miguel A. Morón

    2014-09-01

    Full Text Available Description of the third instar larvae of five species of Cyclocephala (Coleoptera, Melolonthidae, Dynastinae from Mexico. Larvae of four species of Cyclocephala are described for the first time based on specimens collected in Mexican localities: C. barrerai Martínez, 1969 from Puebla, C. sinaloae Howden & Endrödi, 1966 from Sinaloa, C. fasciolata Bates, 1888 from Veracruz, and C. jalapensis Casey, 1915 from Hidalgo. Larva of C. lunulata Burmeister, 1847, is redescribed based on specimens from the Mexican states of Morelos, Puebla, and Veracruz. Diagnostic structures are illustrated and the differences and similarities of each species with other previously described larvae of the genus are commented.

  20. Espécies de Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini ocorrentes na Bolívia

    Directory of Open Access Journals (Sweden)

    Maria Helena M. Galileo

    2013-03-01

    Full Text Available Species of Gorybia Pascoe (Coleoptera, Cerambycidae, Piezocerini occurring in Bolivia. The genus Gorybia (Cerambycinae, Piezocerini consists of 45 described species with seven species recorded from Bolivia. Nine new species are described herein from Bolivia: G. abnormalis sp. nov.; G. alveolata sp. nov.; G. asyka sp. nov.; G. florida sp. nov.; G. inarmata sp. nov.; G. longithorax sp. nov.; G. guenda sp. nov.; G. tuberosa sp. nov. and G. wappesi sp. nov. A key to the species now known to occur in Bolivia is included.

  1. On the family- and genus-series nomina in Gyrinidae Latreille, 1810 (Coleoptera, Adephaga).

    Science.gov (United States)

    Gustafson, Grey T; Miller, Kelly B

    2013-01-01

    All available genus- and family- group nomina for the Gyrinidae (Coleoptera: Adephaga) are listed along with original citation, original and current status, type nominal taxon with method of designation, and known synonymies and incorrect subsequent spellings. The nomina included follow the most current classification. Discussion is provided clarifying numerous nomenclatural problems with original spellings, correct authorship and type designation. Dineutini Ochs, 1926 syn. nov. is found to be a junior homonym of Dineutini Desmarest, 1851, and Enhydrini Régimbart, 1882 syn. nov. and its justified emendation Enhydrusini (Anonymous 2012) are here synonymized with Dineutini Desmarest, 1851. PMID:25277555

  2. Gross anatomy of central nervous system in firefly, Pteroptyx tener (Coleoptera: Lampyridae)

    Science.gov (United States)

    Hudawiyah, Nur; Wahida, O. Nurul; Norela, S.

    2015-09-01

    This paper describes for the first time the organization and fine structure of the central nervous system (CNS) in the fireflies, Pteroptyx tener (Coleoptera: Lampyridae). The morphology of the CNS was examined by using Carl Zeiss AxioScope A1 photomicroscope with iSolution Lite software. Some specific structural features such as the localization of protocerebrum, deutocerebrum and tritocerebrum in the brain region were analyzed. Other than that, the nerve cord and its peripheral structure were also analyzed. This study suggests that, there is a very obvious difference between male and female central nervous system which illustrates that they may differ in function in controlling physiological and behavioral activities.

  3. Soil and saproxylic species (Coleoptera, Collembola, Araneae in primeval forests from the northern part of South-Easthern Carpathians

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    Eugen Nitu

    2009-11-01

    Full Text Available In 2006-2007 we carried out faunal investigations in the vernal, estival and autumnal seasons in the scientific reserve "Codrul Secular Giumalãu" using quantitative sampling methods. We identified 189 species of Coleoptera, 70 of Collembola and 20 of Araneae. Of these, 11 phytophagous, 18 myceto/xylo-mycetophagous,9 mixophagous, 18 xylo- and cambio-xylemophagous, 38 saproxylophagous,125 (55 Coleoptera, 70 Collembola detritivorous (sapro-, copro- andnecrophagous, 60 (40 Coleoptera, 20 Aranea predators/parasitoids. Hymenaphorura polonica Pomorski, 1990 (Collembola, and Leiodes rhaeticus Erichson, 1845 (Coleoptera, Leiodidae, are recorded for the first time in the Romanian fauna. The rare species and characteristic species for the old primeval spruce forests are analysed for each studied taxonomic group. The species richness and faunal diversity from the Giumalãu primeval spruce forest are compared with those of other very well preserved forests from the Carpathians scientific reserves (Codrul Secular Slãtioara,Pietrosul Rodnei. The species abundances were used to compute the similarity indexes between the sampled sectors of forest and to perform Cluster Analysis. We observed that the dead wood in the 2nd-6th phases of decomposition has a great influence not only on the saproxylic species but also on the soil fauna like ground beetles(Carabidae that use the logs as ecologic microrefuges (winter refugees or diurnal refugees. The structure of the soil fauna is influenced by wood extraction from the forest ecosystem or by natural perturbations, this consisting in the appearance of opportunistic species as Orchesella pontica (Collembola and in decreasing ofspecies richness of Carabidae (Coleoptera.

  4. Construction of an environmental safe Bacillus thuringiensis engineered strain against Coleoptera.

    Science.gov (United States)

    Yu, Yajun; Yuan, Yihui; Gao, Meiying

    2016-05-01

    Cloning of new toxic genes from Bacillus thuringiensis (Bt) and construction of Bt engineered strains are two key strategies for bio-control of coleopteran pests in agriculture and forestry. In this study, we cloned a new cry3Aa-type gene, cry3Aa8, from wild Bt strain YC-03 against coleopteran, and constructed a Bt engineered strain, ACE-38, containing insecticidal protein-encoding gene cry3Aa8. The engineered strain, with almost four times of Cry3Aa yield compared with strain YC-03, was an antibiotic marker-free strain. Though no selective pressure was presented in the medium, cry3Aa8 in the engineered strain ACE-38 remained stable. The yield of Cry3Aa by strain ACE-38 reached 2.09 mg/ml in the optimized fermentation medium. The activity of strain ACE-38 against Plagiodera versicolora was tested, and the LC50 of ACE-38 cultures in the optimized fermentation medium was 1.13 μl/ml. Strain ACE-38 is a non-antibiotic Bt engineered strain with high Chrysomelidae toxicity and exhibits good fermentation property. The modified indigenous site-specific recombination system constructed in this study might be useful for the construction of Bt engineered strains containing genes that cannot be expressed in the indigenous site-specific recombination system using plasmid pBMB1205R. PMID:26767987

  5. The hydraulic mechanism in the hind wing veins of Cybister japonicus Sharp (order: Coleoptera).

    Science.gov (United States)

    Sun, Jiyu; Wu, Wei; Ling, Mingze; Bhushan, Bharat; Tong, Jin

    2016-01-01

    The diving beetles (Dytiscidae, Coleoptera) are families of water beetles. When they see light, they fly to the light source directly from the water. Their hind wings are thin and fragile under the protection of their elytra (forewings). When the beetle is at rest the hind wings are folded over the abdomen of the beetle and when in flight they unfold to provide the necessary aerodynamic forces. In this paper, the unfolding process of the hind wing of Cybister japonicus Sharp (order: Coleoptera) was investigated. The motion characteristics of the blood in the veins of the structure system show that the veins have microfluidic control over the hydraulic mechanism of the unfolding process. A model is established, and the hind wing extending process is simulated. The blood flow and pressure changes are discussed. The driving mechanism for hydraulic control of the folding and unfolding actions of beetle hind wings is put forward. This can assist the design of new deployable micro air vehicles and bioinspired deployable systems. PMID:27547607

  6. Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures

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    César Murilo de Albuquerque Correa

    2013-03-01

    Full Text Available Dung beetles (Coleoptera, Scarabaeinae attracted to sheep dung in exotic pastures. In this study we provide data on the abundance and richness of dung beetles (Coleoptera, Scarabaeidae, Scarabaeinae attracted to sheep dung in exotic pastures (Brachiaria spp.. In four areas of exotic pasture pitfall traps were installed and baited with fresh sheep dung for sampling of dung beetles. A total of 2,290 individuals were captured belonging to 16 species, 10 genera and five tribes of Scarabaeinae beetles. Trichillum externepunctatum Preudhomme de Borre, 1886 and Dichotomius bos (Blanchard, 1843 were dominant. The guild of dwellers was the most abundant in pastures. We demonstrate that dung beetles are attracted to sheep dung. Since the production of both cattle and sheep in the same area is common in tropical pasturelands, results obtained here highlight the need to investigate the actual role of dung sharing (cattle dung + sheep dung by dung beetles. It is also suggested that experiments be performed for evaluation of the ecological functions performed by dung beetles using sheep dung.

  7. Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain.

    Science.gov (United States)

    Romón, Pedro; Zhou, XuDong; Iturrondobeitia, Juan Carlos; Wingfield, Michael J; Goldarazena, Arturo

    2007-06-01

    Bark beetles (Coleoptera: Scolytinae) are known to be associated with fungi, especially species of Ophiostoma sensu lato and Ceratocystis. However, very little is known about these fungi in Spain. In this study, we examined the fungi associated with 13 bark beetle species and one weevil (Coleoptera: Entiminae) infesting Pinus radiata in the Basque Country of northern Spain. This study included an examination of 1323 bark beetles or their galleries in P. radiata. Isolations yielded a total of 920 cultures, which included 16 species of Ophiostoma sensu lato or their asexual states. These 16 species included 69 associations between fungi and bark beetles and weevils that have not previously been recorded. The most commonly encountered fungal associates of the bark beetles were Ophiostoma ips, Leptographium guttulatum, Ophiostoma stenoceras, and Ophiostoma piceae. In most cases, the niche of colonization had a significant effect on the abundance and composition of colonizing fungi. This confirms that resource overlap between species is reduced by partial spatial segregation. Interaction between niche and time seldom had a significant effect, which suggests that spatial colonization patterns are rarely flexible throughout timber degradation. The differences in common associates among the bark beetle species could be linked to the different niches that these beetles occupy. PMID:17668036

  8. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

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    PEDRO G. DA SILVA

    2013-05-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante' (MOEL, ‘Morro do Cerrito' (MOCE and ‘Campus da Universidade Federal de Santa Maria' (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e abund

  9. Diversity and seasonality of Scarabaeinae (Coleoptera: Scarabaeidae in forest fragments in Santa Maria, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    PEDRO G. DA SILVA

    2013-06-01

    Full Text Available Scarabaeinae specimens were collected with the use of pitfall traps baited with human excrement, rotten meat and rotting banana, between May 2009 and April 2010, in three forest fragments in Santa Maria, Rio Grande do Sul, Brazil: ‘Morro do Elefante’ (MOEL, ‘Morro do Cerrito’ (MOCE and ‘Campus da Universidade Federal de Santa Maria’ (UFSM. A total of 19,699 individuals belonging to 33 species were collected. Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. and Canthon lividus Blanchard, 1845, were the most abundant species. MOEL showed the greatest richness, MOCE the greatest abundance, while UFSM showed the lowest values of richness and abundance. The greatest similarity (qualitative and quantitative was found between MOEL and MOCE, while the lowest occurred between MOCE and UFSM. Only 51% of the species were common to all three fragments. The richness and abundance of Scarabaeinae were positively correlated with the air temperature. The richness of the fragment decreased according to the smaller size and the greater degree of disturbance of vegetation structure.Espécimes de Scarabaeinae foram coletados com o uso de armadilhas de queda iscadas com excremento humano, carne apodrecida e banana em decomposição, entre maio de 2009 e abril de 2010, em três fragmentos florestais em Santa Maria, Rio Grande do Sul, Brasil: Morro do Elefante (MOEL, Morro do Cerrito (MOCE e Campus da Universidade Federal de Santa Maria (UFSM. Um total de 19.699 indivíduos de 33 espécies foi coletado; Canthidium aff. trinodosum Boheman, 1858, Canthon latipes Blanchard, 1845, Dichotomius assifer (Eschscholtz 1822, Eurysternus caribaeus (Herbst 1789, Canthidium sp. e Canthon lividus Blanchard, 1845 foram as espécies mais abundantes. MOEL apresentou a maior riqueza, MOCE a maior abundância, enquanto UFSM teve os menores valores de riqueza e

  10. ESCARABAJOS TIGRE (COLEOPTERA: CICINDELIDAE DEL MUSEO ENTOMOLÓGICO FRANCISCO LUÍS GALLEGO: NUEVOS REGISTROS PARA DEPARTAMENTOS DE COLOMBIA TIGER BEETLES (COLEOPTERA: CICINDELIDAE AT THE ENTOMOLOGICAL MUSEUM FRANCISCO LUÍS GALLEGO: NEW RECORDS

    Directory of Open Access Journals (Sweden)

    Manuel Alejandro Ramírez Mora

    2008-06-01

    Full Text Available Se registran por primera vez las especies de escarabajos tigre (Coleoptera: Cicindelidae presentes en el Museo Entomológico Francisco Luis Gallego (MEFLG. Se identificaron 167 especimenes distribuidos en ocho géneros y 27 especies, se reportan 24 nuevos registros para diferentes departamentos de Colombia. Se señalan aspectos importantes de la taxonomía y sistemática del grupo. Además, se presentan comentarios biológicos y de distribución de las especies.Tiger beetles (Coleoptera: Cicindelidae species at the Museo Entomológico Francisco Luis Gallego (MEFLG are registered by first time. 167 specimens in 8 genus and 27 species were identified, 24 new records for different Colombian states are reported. Some important aspects of the group’s taxonomy and systematic are pointed. Additionally, species’ biological and distributional comments are presented.

  11. Survival of boll weevil (Coleoptera: Curculionidae)adults after feeding on pollens from various sources

    Institute of Scientific and Technical Information of China (English)

    SHOIL M. GREENBERG; GRETCHEN D. JONES; FRANK EISCHEN; RANDY J.COLEMAN; JOHN J. ADAMCZYK, JR; TONG-XIAN LIU; MAMOUDOU SETAMOU

    2007-01-01

    The survival of overwintering boll weevil, Anthonomus grandis grandis (Boheman),adults on non-cotton hosts in the Lower Rio Grande Valley (LRGV) of Texas was examined from 2001 to 2006. The success of the Boll Weevil Eradication Program, which was reintroduced into the LRGV in 2005, depends on controlling overwintering boll weevil populations. Laboratory studies were conducted using boll weevil adults that were captured in pheromone traps from September through March. The number of adults captured per trap declined significantly in the field from fall to the beginning of spring (3.5-7.0-fold). The proportion of trapped males and females did not differ significantly. The mean weight of boll weevil adults captured in September was 13.3 mg, while those of captured adults from November to February were significantly lower and ranged from 6.7 to 7.8 mg. Our results show that boll weevil adults can feed on different plant pollens. The highest longevity occurred when adults were fed almond pollen or mixed pollens (72.6 days and 69.2 days, respectively)and the lowest when they fed on citrus pollen or a non-food source (9.7 days or 7.4 days,respectively). The highest adult survival occurred on almond and mixed pollens [88.0%-97.6% after 1st feeding period (10 days), 78.0%-90.8% after 3rd feeding period (10 days), 55.0%-83.6% after 5th feeding period (10 days), and 15.2%-32.4% after 10th feeding period (10days)]. The lowest adult survival occurred on citrus pollen [52.0%-56.0% after 1st feeding period (10 days), 13.3% after 3rd and 5th feeding periods (10 days), and 0 after 6th feeding period (10 days)]. Pollen feeding is not a behavior restricted to adult boll weevils of a specific sex or physiological state. Understanding how boll weevil adults survive in the absence of cotton is important to ensure ultimate success of eradicating this pest in the subtropics.

  12. Especies de Megacerus (Coleoptera: Bruchidae y sus plantas hospederas en Yucatán Megacerus species (Coleoptera: Bruchidae and their host plants in Yucatán

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    Enrique Reyes

    2009-12-01

    Full Text Available Se registra la incidencia de Megacerus (Coleoptera: Bruchidae en semillas de 7 especies de Convolvulaceae de 6 sitios al norte de Yucatán. Las semillas de Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia y M. cissoides recolectadas en etapa de dispersión fueron colocadas en sobres limpios de papel y puestas en condiciones de laboratorio hasta la emergencia de los brúquidos. Se obtuvieron 1111 coleópteros pertenecientes a 5 especies: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, y M. (Serratibruchus cubiciformis. Las semillas de I. crinicalyx, M. aegyptia y M. cissoides fueron infestadas principalmente por M. (M. porosus, las de I. hederifolia e I. triloba por M. (M. cubiculus y las de I. nil por M. (M. tricolor. Todos los individuos de Megacerus (M. sp., fueron encontrados en semillas de J. penthanta. En el nivel génerico de hospedero se encontró que las semillas de Ipomoea fueron infestadas principalmente por M. (M. cubiculus y las de Merremia por M. (M. porosus. Estos resultados amplían el rango de plantas hospederas registrado para las especies de Megacerus y resaltan la estrecha asociación que existe entre un linaje de depredadores de semillas Bruchidae y plantas de la familia Convolvulaceae.Seed-attacking by Megacerus (Coleoptera: Bruchidae is reported for 7 Convolvulaceae species from 6 sites at the North of Yucatan. Seeds of Ipomoea crinicalyx, I. hederifolia, I. nil, I. triloba, Jacquemontia penthanta, Merremia aegyptia and M. cissoides were collected and placed in clean envelopes under laboratory conditions to collect all bruchids emerged from seeds. A total of 1111 coleopterans were hatched from seeds, and 5 taxa were identify: Megacerus (Megacerus cubiculus, M. (M. porosus, Megacerus (M. sp., M. (M. tricolor, and M. (Serratibruchus cubiciformis. Seeds of I. crinicalyx, M. aegyptia and M. cissoides were attacked mainly by M. (M. porosus; I

  13. Longhorned Beetles Collection of the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey (Coleoptera,Cerambycidae)

    OpenAIRE

    ÖZDİKMEN, Hüseyin; ŞAHİN, Özlem

    2010-01-01

    In the present paper, specimens of Cerambycidae were examined in the Entomology Museum of Central Anatolia Forestry Research Directorship, Ankara, Turkey. As a result of identification of these specimens, thirty-eight species and two subspecies belonging to twenty-eight genera of five subfamilies were determined. With this paper, new faunistic data and some zoogeographical evaluations were presented on longhorned beetles fauna (Coleoptera, Cerambycidae) of Turkey. The faunistic data in the pr...

  14. Synergistic effects of chlorpyrifos with piperonyl butoxide (pbo) against the lesser mealworm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae)

    OpenAIRE

    Akter Mst Yeasmin; Talukdar Muhammad Waliullah; ASM Shafiqur Rahman

    2014-01-01

    Objective: To investigate the co-toxicity and co-efficient activity of Chlorpyrifos (Dursban 20EC), an organophosphate and Piperonyl butoxide (PBO) against the lesser meal worm Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) (A. diaperinus). Methods: The repellent activity was carried out by the residual film assay technique. Statistically the dose mortality relationship was expressed as a median lethal dose (LD50) by the probit analysis. The regression lines and isoboles were ...

  15. Natural history of Belonuchus Nordmann spp. and allies (Coleoptera: Staphylinidae) in Heliconia L. (Zingiberales: Heliconiaceae) flower bracts

    OpenAIRE

    Frank, J. Howard; Barrera, Roberto

    2010-01-01

    Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larva...

  16. Uji Efektifitas Beberapa Insektisida Nabati Terhadap Mortalitas Hama Sitophilus zeamais Motsch. (Coleoptera; Curculionidae) Pada Benih Jagung (Zea mays)

    OpenAIRE

    Subhan, Fadillah

    2011-01-01

    Fadillah Subhan, "Test Effectiveness of Some Insecticides Against Plant Pests Sitophilus zeamais Motsch Mortality. (Coleoptera: Curculionidae) In the Seed of Corn (Zea mays). "It was under supervised by Yuswani Pangestiningsih and Fatimah Zahara. S. zeamais is a pest of corn in storage that causes substantial yield losses, one of which effective control is the use of botanical insecticides. Testing the effectiveness of some botanical insecticides on mortality of S. zeamais on maize seed plant...

  17. A review of Elocomosta Hansen with a description of a new species with reduced eyes from China (Coleoptera, Hydrophilidae, Sphaeridiinae)

    Science.gov (United States)

    Lin, Renchao; Jia, Fenglong; Fikáček, Martin

    2016-01-01

    Abstract A new species of the genus Elocomosta Hansen, 1989 (Coleoptera: Hydrophilidae: Sphaeridiinae: Coelostomatini), Elocomosta lilizheni sp. n., is described from Guangxi Province, China. It is compared in detail with the only other known species of the genus, Elocomosta nigra Hansen, 1989 from Borneo, and the genus is diagnosed from the remaining coelostomatine genera. The new species is unusual among Hydrophilidae by having extremely reduced eyes. PMID:27551232

  18. Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae)

    OpenAIRE

    El-Sayed, Wael S.; Ibrahim, Reda A

    2015-01-01

    Background The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in food digestion and insecticide resistance mechanisms, and therefore are essential to their host insect. Identification of these bacteria in their insect host can lead to developme...

  19. First record of Rhoptrocentrus piceus Marshall (Hymenoptera, Braconidae, Doryctinae as parasitoid of Psacothea hilaris hilaris (Pascoe (Coleoptera, Cerambycidae

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    Augusto Loni

    2015-02-01

    Full Text Available The species Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe (Coleoptera: Cerambycidae, an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  20. First record of Rhoptrocentrus piceus Marshall ( Hymenoptera , Braconidae , Doryctinae ) as parasitoid of Psacothea hilaris hilaris (Pascoe) ( Coleoptera , Cerambycidae )

    OpenAIRE

    Augusto Loni; Costanza Jucker; Sergey Belokobylskij; Daniela Lupi

    2015-01-01

    Abstract The species Rhoptrocentrus piceus Marshall ( Hymenoptera : Braconidae ) was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe) ( Coleoptera : Cerambycidae ), an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.

  1. Sublethal effects of malathion on boll weevil (Coleoptera:Curculionidae) fecundity when maintained on cotton squares or artificial diet

    Institute of Scientific and Technical Information of China (English)

    JOHN SCOTT ARMSTRONG; ALLAN T. SHOWLER; MAMOUDOU SETAMOU; SHOIL GREENBERG

    2006-01-01

    Mated 3-day-old female boll weevils, Anthonomus grandis grandis Boheman,reared from field-infested cotton (Gossypium hirsutum L.) squares were topically treated with an estimated LD50 of malathion (2μg) to assess its effects on fecundity, oviposition, and body fat condition. Two different food sources, cotton squares and artificial diet, were assessed in malathion-treated and nontreated (control) weevils. The LD50 caused ≈ 50%mortality in the square-fed malathion treatment, but the artificial diet-fed malathion-treated weevils were less susceptible. LD50 survivors fed on the squares produced ≥ 9 times more chorionated eggs in the ovaries and oviposited≥ 19-fold more than survivors fed artificial diet, regardless of the malathion treatment. Boll weevils that survived a 2μg LD50 malathion and also fed squares were ≈ 4.5-fold leaner than diet-fed weevils. Our findings demonstrate that non-resistant boll weevils surviving a sublethal dose of malathion will reproduce without any delay or significant loss in fecundity, and the food source for which boll weevils are maintained when conducting these assays will directly affect the results. The significance of these findings and how they are related to the final stages of eradicating the boll weevil from the US are discussed.

  2. Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

    DEFF Research Database (Denmark)

    Lövei, G. L.; Magura, T.

    2015-01-01

    The role of hedgerows in supporting ground beetles (Coleoptera: Carabidae) in a Danish agricultural landscape was examined. Nine old, well established single-row hedges were selected for the study, three each of a native species (hawthorn, Crataegus monogyna), a non-native deciduous one (rowan...... beetle assemblages. The number of ground beetle individuals and species were significantly the highest in the hawthorn hedges and significantly decreased from the hedges with rowan toward the spruce hedges. The elevated number of ground beetle individuals and species in the hawthorn hedges were due...... to the forest specialist species, as the number of forest specialist ground beetle individuals and species were significantly higher in the hawthorn hedges compared to the hedges with rowan and spruce. Differences in the number of the grassland and the cropland specialist ground beetle individuals and species...

  3. A importância forense de Oxelytrum discicolle (Brullé, 1840) (Coleoptera, Silphidae)

    OpenAIRE

    Camila Kraiczyi Kotzko; V. Costa-Silva; E. G. Silva

    2015-01-01

    Oxelytrum discicolle Brullé, 1840 é a espécie mais coletada de Silphidae (Coleoptera) na América Latina, constituindo um indicador forense na região Neotropical. O objetivo do trabalho é reunir informações sobre biologia e distribuição de O. discicolle, além de ferramentas para utilização desse besouro como indicador forense em casos de morte de humanos. O. discicolle é um besouro de grande porte, de cor preta com um colar vermelho. Larvas da espécie são necrófagas, já os adultos são onívoros...

  4. The first complete mitochondrial genome of stag beetle from China, Prosopocoilus gracilis (Coleoptera, Lucanidae).

    Science.gov (United States)

    Wu, Yuan-Yuan; Cao, Yu-Yan; Fang, Jie; Wan, Xia

    2016-07-01

    The complete mitochondrial genome (mitogenome) of Prosopocoilus gracilis (Coleoptera: Lucanidae) that is endemic to Southern China is determined. The circular genome is 736 bp in length and comprises 13 protein-coding genes, 22 tRNA, 2 rRNA genes and a control region. Gene order is identical to that of the putative ancestral arrangement of insects. The nucleotide composition of heavy strand is A (36.6%), C (22.6%), T (29.5%) and G (11.3%). All protein-coding genes start with a typical ATN codon except for the gene COI that uses AAC as the start codon. tRNA-Ser (AGN) uses the anticodon UCU instead of the commonly used GCU. Both maximum likelihood and Bayesian analyses support the monophyly of Lucanidae and the sister relationship of Nigidionus and the remaining sampled genera. Two species of Prosopocoilus were not recovered as a monophyletic group. PMID:26024142

  5. The mortality of Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae induced by powdered plants

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    Kłyś Małgorzata

    2015-01-01

    Full Text Available The aim of this study was to investigate whether powdered plants of different species namely: peppermint Mentha piperita (L. (Lamiaceae, wormwood Artemisia absinthium (L. (Asteraceae, common sage Salvia officinalis (L. (Lamiaceae, allspice Pimenta dioica (Linnaeus et Merrill (Myrtaceae and common garlic Allium sativum (L. (Amaryllidaceae, added to semolina using concentrations of 1.23, 3.61, and 5.88%, influence the mortality rate in the saw-toothed grain beetle Oryzaephilus surinamensis Linnaeus, 1758 (Coleoptera: Silvanidae. Experiments were conducted in a laboratory at 28°C and relative humidity 60±5%. At the concentration of 1.23%, allspice seeds caused the highest mortality amongst the saw-toothed grain beetle. When concentrations of 3.61 and 5.88% were used, sage, peppermint and wormwood caused the highest statistically significant mortality of O. surinamensis

  6. Communication disruption using synthetic sex pheromone for control of sugarcane wireworm. Melanotus okinawensis Ohira (Coleoptera: Elateridae)

    International Nuclear Information System (INIS)

    We tested communication disruption through the use of a synthetic sex pheromone from mid February to early June 2001 at the cultivating land on Minami-Daito Island in order to control the sugarcane wireworm Melanotus okinawensis Ohira (Coleoptera: Elateridae). Although very few males were caught in monitoring traps except a few traps set in a treated area, a large number were caught in traps set in an untreated Japanese pampas grass field. This indicates that communication disruption was effective throughout the period in the treated area. Females collected from treated area of Minami-Daito Island, mating rates that were lower than those of females in the untreated areas on Miyagi Island. We therefore concluded that a communication disruption system is an effective tool for the sugarcane wireworm management. (author)

  7. What do we know about winter active ground beetles (Coleoptera, Carabidae in Central and Northern Europe?

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    Radomir Jaskula

    2011-05-01

    Full Text Available This paper summarizes the current knowledge on winter active Carabidae in Central and Northern Europe. In total 73 winter active species are listed, based on literature and own observations. Ground beetles are among the three most numerous Coleoptera families active during the autumn to spring period. The winter community of Carabidae is composed both of larvae (mainly autumn breeding species and adults, as well as of epigeic species and those inhabiting tree trunks. Supranivean fauna is characterized by lower species diversity than the subnivean fauna. The activity of ground beetles decreases in late autumn, is lowest during mid-winter and increases in early spring. Carabidae are noted as an important food source in the diet of insectivorous mammals. They are also predators, hunting small winter active invertebrates.

  8. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    Science.gov (United States)

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  9. Taxonomy of Colophon Gray (Coleoptera: Lucanidae): new species and a status change.

    Science.gov (United States)

    Jacobs, Carmen T; Scholtz, Clarke H; Strümpher, Werner P

    2015-01-01

    Three new species of the Cape high-mountain stag beetle genus, Colophon Gray (Coleoptera: Lucanidae), from South Africa are described. They are C. deschodti new species, C. switalae new species, and C. struempheri new species. The new taxa fall within a species complex of geographically disjunct entities related to Colophon stokoei Barnard. Furthermore, the mitochondrial COI gene shows a high degree of sequence divergence, with pairwise genetic distances between the species ranging between 7.4-10.7%. The new species are illustrated by photographs. Colophon eastmani nagaii Mizukami is raised to species level on the basis of geographic range and molecular differences between it and the nominate subspecies. This brings the total number of described species in the genus to 21. An updated checklist of the South African species of Colophon is also provided. PMID:26701471

  10. Carpophilus zeaphilus, a new sap beetle species acclimatized in Italy (Coleoptera: Nitidulidae

    Directory of Open Access Journals (Sweden)

    Paolo Audisio

    2015-06-01

    Full Text Available Carpophilus zeaphilus Dobson, 1969 (Coleoptera, Nitidulidae, Carpophilinae is an Afrotropical species that has become widespread in Sub-Saharan Africa, the Arabian Peninsula, and southern Mediterranean areas in recent years. The species was first recorded from Europe in Portugal and Spain nearly thirty years ago, and it was later intercepted in Sicily near Trapani in 1991. A few specimens of this species were collected in April, 2015 in a sparsely forested area near Rome, which suggests a recent acclimatization into peninsular Italy. Specimens were taken on flowering trees of Prunus spinosa L. (Rosaceae, an unusual occurrence for most introduced species of Carpophilinae that are normally associated with rotten fruit and other decomposing vegetal matter.

  11. Hemocyte Responses of the Colorado Potato Beetle, Leptinotarsa decemlineata, and the Greater Wax Moth, Galleria mellonella, to the Entomopathogenic Nematodes, Steinernema feltiae and Heterorhabditis bacteriophora

    OpenAIRE

    Ebrahimi, L; Niknam, G.; Dunphy, G. B.

    2011-01-01

    Hemocyte encapsulation reactions of infective juveniles of two Iranian isolates of the entomopathogenic nematodes, Heterorhabditis bacteriophora Poinar (Rhabditina: Heterorhabditidae) and Steinernema feltiae Filipjev (Tylenchina: Steinernematidae), were compared in the economic pest Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), and the greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae). The former was a more responsive host than the latter a...

  12. Gastrophysa polygoni herbivory on Rumex confertus: Single leaf VOC induction and dose dependent herbivore attraction/repellence to individual compounds

    Science.gov (United States)

    We report large induction (> 65fold increases) of volatile organic compounds (VOCs) emitted from a single leaf of the invasive weed mossy sorrel, Rumex confertus Willd. (Polygonaceae), by herbivory of the dock leaf beetle, Gastrophysa polygoni L. (Coleoptera: Chrysomelidae). The R. confertus VOC ble...

  13. Spatial clustering of Diabrotica virgifera virgifera and Agriotes ustulatus in small-scale maize fields without topographic relief drift

    Science.gov (United States)

    The soil living larvae of Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) and Agriotes ustulatus Schaller (Elateridae) can cause economic damage to maize roots. This study investigated the spatial clustering of both pests in four small-scale maize fields in southern Hungary, wher...

  14. Chemical control of Diabrotica virgifera virgifera LeConte

    NARCIS (Netherlands)

    Rozen, van K.; Ester, A.

    2010-01-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is one of the most important pest species of maize in several countries of Central and Eastern Europe. This pest insect has invaded from the USA and is mainly controlled by insecticides in the EU. In the U

  15. Comparison of Laboratory and Ecological Host Range of the Saltcedar Leaf Beetle with Respect to Native Non-Target Frankenia species

    Science.gov (United States)

    Laboratory and field host specificity tests were conducted with the saltcedar biocontrol agent, Diorhabda elongata (Coleoptera: Chrysomelidae) from Crete, to assess the potential risk of impact to non-target North American Frankenia species. Larval survival was not significantly different between T...

  16. Bacillus thuringiensis Cry3Aa toxin increases the susceptibility of Crioceris quatuordecimpunctata to Beauveria bassiana infection

    Science.gov (United States)

    The spotted asparagus beetle, Crioceris quatuordecimpunctata (Coleoptera: Chrysomelidae), is one of the most devastating pests of asparagus in China and elsewhere. In this study, we investigated the interaction of Bacillus thuringiensis (Bt) Cry3Aa toxin and the entomopathogenic fungus Beauveria bas...

  17. Geographic information systems in corn rootworm management

    Science.gov (United States)

    Corn rootworms (Diabrotica spp. Coleoptera: Chrysomelidae) are serious pests of corn (Zea mays) in the United States and Europe. Control measures for corn rootworms (CRW) were historically based upon chemical pesticides and crop rotation. Pesticide use created environmental and economic concerns. In...

  18. AFLP markers for the R-gene in the flea beetle, Phyllotreta nemorum, conferring resistance to defenses in Barbarea vulgaris

    NARCIS (Netherlands)

    Breuker, C.J.; Victoir, K.; Jong, de P.W.; Meijden, van der E.; Brakefield, P.M.; Vrieling, K.

    2005-01-01

    A so-called R-gene renders the yellow-striped flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae: Alticinae) resistant to the defenses of the yellow rocket Barbarea vulgaris R.Br. (Brassicacea) and enables it to use it as a host plant in Denmark. In this study, genetic markers for an auto

  19. Diversidade de Carabidae (Coleoptera) Amostrados em Áreas de Reflorestamento de Mata Ciliar e Fragmento Florestal, no Estado do Paraná

    OpenAIRE

    Tamara Quinteiro; José Lopes; Ivan Martins

    2012-01-01

    Carabidae são Coleoptera com a maioria de suas espécies apresentando hábito alimentar predatório. O objetivo do presente trabalho foi estudar a diversidade e abundância da família Carabidae (Coleoptera) em áreas de fragmento florestal e em áreas de reflorestamento de mata ciliar, evidenciando a importância deste grupo como indicador da biodiversidade em relação ao sucesso do reflorestamento. Os besouros foram amostrados por meio de armadilhas de solo pitfall, em fragmentos florestais e reflor...

  20. Locomotor activity of Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae on Chilean sandy beaches Actividad locomotora de Phalerisida maculata Kulzer (Coleoptera, enebrionidae en playas arenosas chilenas

    Directory of Open Access Journals (Sweden)

    EDUARDO JARAMILLO

    2000-03-01

    Full Text Available The locomotor activity of the beetle Phalerisida maculata Kulzer (Coleoptera Tenebrionidae was studied on the surface of the substrate in two sandy beaches of the Chilean coast: one in north central (ca. 29ºS and the other in south central Chile (ca.39ºS. During the summer period of 1991 the circadian locomotor activity was studied in the southern beach, while during that of 1997 in both beaches. To analyze the activity, pitfall traps were used which were ordered along two transects extended between the upper beach and the resurgence zone. The traps were checked (i.e. collection of captured insects every two hours for a total period of 26 hours. The results showed that the adults of P. maculata were mostly active during the night hours, whereas the larvae were active during both, the day and night. Studies carried out in the beach located in south central Chile show that differences in the tidal range (neap vs. spring tides do not affect the activity patterns. During the locomotor activity, adult and larvae move to lower intertidal levels than those usually occupied while buried. Results of laboratory experiments using actographs under conditions of darkness and constant temperature, suggest that adults and larvae of P. maculata presented a circadian rhythm similar to that observed in the field experiments. It is concluded that P. maculata presents a behaviour that appear to be under control of an endogenous rhythm, without showing differences in the circadian rhythm of activity when beaches located at different latitudes are comparedSe estudió la actividad locomotriz del escarabajo Phalerisida maculata Kulzer (Coleoptera, Tenebrionidae sobre la superficie del sustrato de dos playas arenosas de la costa de Chile: una en el centro norte (ca. 29ºS y otra en el centro sur (ca. 39ºS. Durante el período estival de 1991 se estudió la actividad locomotriz circadiana en la playa del centro sur y durante el de 1997 en ambas playas. Para analizar

  1. Nuevas citas de Coleoptera para la Argentina (Carabidae, Lucanidae, Scarabaeidae y Tenebrionidae New records of Coleoptera for Argentina (Carabidae, Lucanidae, Scarabeidae and Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sergio Roig-Juñent

    2004-12-01

    Full Text Available En enero de 2003, se realizó un viaje de campaña con el fin de recolectar materiales en áreas poco prospectadas de la estepa patagónica de Mendoza y Neuquén, Argentina, así como en ambientes de bosques de Nothofagus en la provincia de Neuquén. Las recolecciones se realizaron en forma manual, usando trampas con cebo y trampas de luz de mercurio. El material recolectado permite citar por primera vez para la Argentina los géneros Nothobroscus Roig-Juñent & Ball y Mimophilorizus Mateu (Carabidae, Callyntra Solier y Homocyrtus Reitter (Tenebrionidae. Además, se citan por primera vez para la Argentina las especies Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier y Brachysternus marginatus (Germain (Scarabeidae y Pycnosiphorus philippii (Westwood (Lucanidae. Se provee una breve descripción de los ambientes en donde estas especies fueron encontradas.A short expedition to northern Patagonia was made in January 2003. The main goal of the trip was to survey the diversity of some families of Coleoptera in the insufficient explored steppes in Mendoza and Neuquén provinces, Argentina and Nothofagus forests of Neuquén province. The techniques included manual collection and the use of different kinds of traps, such as with bite or artificial light of mercury. The study of the material revealed four new genera records and four new species records for the country: Nothobroscus Roig-Juñent & Ball and Mimophilorizus Mateu (Carabidae, Callyntra Solier and Homocyrtus Reitter (Tenebrionidae are the new genus records. Cnemalobus sulciferus Philippi (Carabidae, Allidiostoma landbecki (Philippi, Macrodactylus chilensis Solier, and Brachysternus marginatus (Germain (Scarabeidae, and Pycnosiphorus philippii (Westwood (Lucanidae are the new species records. A brief description of the habitats where the species were collected is provided.

  2. Preliminary evaluation of Delphastus catalinae (Coleoptera:Coccinellidae)as a predator of the ficus whitefly, Singhiella simplex (Singh) (Hemiptera:Aleyrodidae)

    Science.gov (United States)

    The predatory lady beetle Delphastus catalinae (Horn) (Coleoptera: Coccinellidae) was evaluated as a potential biological control agent against the ficus whitefly, Singhiella simplex (Singh) (Hemiptera: Aleyrodidae), a newly-invasive pest of ficus plants. Adult D. catalinae females were starved for ...

  3. Brood production by Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet based on sawdust of different species

    Science.gov (United States)

    The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the US. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and cont...

  4. A new genus and species of myrmecophilous aphodiine beetle (Coleoptera, Scarabaeidae inhabiting the myrmecophytic epiphyte Platycerium sp. (Polypodiaceae in the Bornean rainforest canopy

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    Munetoshi Maruyama

    2010-01-01

    Full Text Available Pterobius itiokai Maruyama, gen. n. and sp. n., (Coleoptera: Scarabaeidae: Aphodiinae is described from Lambir Hills National Park, Borneo based on specimens collected from a Crematogaster difformis ant nest in the myrmecophytic epiphytic fern genus Platycerium. Pterobius belongs to the tribe Eupariini and is closely related to the Indo-Australian genus Cnematoplatys.

  5. New records of Paracrias Ashmead (Hymenoptera, Eulophidae as parasitoids on weevil larvae (Coleoptera, Curculionidae in Brazil, with the description of a new species

    Directory of Open Access Journals (Sweden)

    Luciano Palmieri

    2013-09-01

    Full Text Available Paracrias strii Schauff, 1985 and P. ceratophaga Palmieri & Hansson sp. nov. are first record in Brazil and both are associated with Ceratopus Schoenherr larvae (Coleoptera, Curculionidae reared from syconia of two species of fig-trees. Both Paracrias species are diagnosed and illustrated. Males of P. ceratophaga sp. nov. are described. The association of Paracrias with weevil larvae is briefly discussed.

  6. Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet

    Science.gov (United States)

    1. Diet and rearing protocols were developed for two members of the cryptic Euwallacea fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB) (Coleoptera: Curculionidae: Scolytinae), using sawdust from boxelder Acer negundo and avocado Persea americana. 2. Bio...

  7. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae) Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae)

    OpenAIRE

    L. S. Fontes; V. Arthur

    1994-01-01

    Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797) (Coleoptera: Tenebrionidae), através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The o...

  8. Desenvolvimento de Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae com Zophobas confusa Gebien (Coleoptera, Tenebrionidae comparado à duas outras presas alternativas Development of Podisus nigrispinus (Dallas (Heteroptera, Pentatomidae fed with Zophobas confusa Gebien (Coleoptera, Tenebrionidae compared with two another alternative preys

    Directory of Open Access Journals (Sweden)

    Teresinha V Zanuncio

    1996-01-01

    Full Text Available Nymphs of Podisus nigrispinus (Dallas, 1851 were fed with Zophobas confusa Gebien, 1906, Tenebrio molitor Linnaeus, 1758 (Coleoptera, Tenebrionidae and Musca domestica Linnaeus, 1758 (Diptera, Muscidae under 25±0.5ºC, 60±10% of RH and photophase of 12h. Nymphal viability of P. nigrispinus were 64.0%, 80.0% and 92.0% with Z. confusa, M. domestica and T. molitor, respectively. No differences were found for pre-oviposition period, number of egg masses, number of eggs, egg viability, and longevity for females of this predator fed with any of these preys.

  9. Tipos de Curculionidae (Coleoptera: Curculionoidea) depositados en la colección entomológica del Museo de La Plata Types of Curculionidae (Coleoptera: Curculionoidea) housed at the Museo de La Plata entomological collection

    OpenAIRE

    Analía A. Lanteri; Suárez, Sonia M.; Guadalupe del Río, M.

    2003-01-01

    Esta contribución referida al material tipo de Curculionidae (Coleoptera: Curculionoidea) depositado en la colección entomológica del Museo de La Plata, actualiza la información sobre los tipos depositados durante los últimos diez años. Los 111 tipos examinados en este trabajo corresponden a 52 especies asignadas a las siguientes subfamilias: Curculioninae (5 spp.), Cryptorhynchinae (8 spp.), Cyclominae (4 spp.), Entiminae (31 spp.), y Erirhininae (4 spp.). Los autores de las especies son C. ...

  10. FACTORES QUE OPTIMIZAN LA EFECTIVIDAD DEL POLVO DE RAÍZ DE Senecio salignus CONTRA EL GORGOJO MEXICANO DEL FRIJOL

    OpenAIRE

    Ernesto López Pérez; Cesáreo Rodríguez Hernández; Ramón Garza García

    2010-01-01

    La raíz de chilca (Senecio salignus DC., Asteraceae) ha mostrado ser efectiva para el control del gorgojo mexicano del frijol (Zabrotes subfasciatus Boheman, Coleoptera: Bruchidae). Sin embargo, falta determinar el efecto de varios factores para hacer un uso racional de este recurso natural y obtener el mejor efecto insecticida. En esta investigación se efectuaron bioensayos para conocer: a) La efectividad de raíz de S. salignus contra Z. subfasciatus a diversos tiempos de almacenamiento; b) ...

  11. Integrated Pest Management for sweetpotato in Eastern Africa.

    OpenAIRE

    Smit, N.E.J.M.

    1997-01-01

    Sweetpotato is an important crop in Eastern Africa. Sweetpotato weevils ( Cylas puncticollis Boheman and C. brunneus Fabricius; Coleoptera: Apionidae) cause damage to roots and vinesthroughout the crop's production area. Other insect pests of sweetpotato are of regional importance. The aim of the research project was to gain insight in the biology and ecology of sweetpotato weevils and, based on this insight, develop pest management programmes on sweetpotato in Eastern Africa.In Chapter 1, th...

  12. Potential for escape of live boll weevils (Coleoptera: Curculionidae) into cottonseed, motes, and cleaned lint at the cotton gin.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-12-01

    Reintroductions of the boll weevil, Anthonomus grandis grandis Boheman, into areas of the United States where it has been eradicated or suppressed are very expensive to mitigate. There is concern that a cotton gin in an eradication zone may serve as a site of boll weevil reintroductions when processing cotton harvested in a neighboring infested zone. Similarly, there is a question whether weevil-free areas can safely import gin products, such as cottonseed and baled lint, from infested areas without risking an introduction. Many countries require fumigation of imported U.S. cotton bales to protect against boll weevil introductions, costing the U.S. cotton industry millions of dollars annually. In previously reported experiments, we quantified the potential for boll weevils to survive passage through precleaning machinery in the gin. In this study, we quantified survival potential of boll weevils passing through the gin stand and segregating into the cottonseed, mote, or lint fractions. We also examined boll weevil survival when passed with ginned lint through a lint cleaner. We present a flow chart of experimentally determined survival potentials of boll weevils passing through the various subprocesses of the gin, from which one can calculate the risk of a live boll weevil reaching any point in the process. Our data show that there is virtually no chance of a boll weevil being segregated alive into the cottonseed or of one surviving in the lint to approach the bale press. Therefore, quarantine or fumigation of cottonseed and cotton bales to guard against boll weevil introductions is unnecessary. PMID:15666727

  13. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  14. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus.

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  15. Coleoptera Scarabaeoidea de la región de Teziutlán, Puebla, México

    OpenAIRE

    Adrián MUÑOZ-HERNÁNDEZ; Morón, Miguel Angel; Agustín ARAGÓN

    2008-01-01

    Con el propósito de evaluar la riqueza, abundancia y fenología de las especies de Coleoptera Scarabaeoidea o Lamellicornia de la región de Teziutlán, Puebla, se realizaron colectas mensuales entre mayo de 1999 y junio de 2000, en sitios con bosques de pino-encino-liquidámbar o zonas de cultivo situados entre 1,990 y 2,000 m de altitud. Se capturaron 2,082 ejemplares que representan a 59 especies de 29 géneros, 18 tribus y 7 subfamilias de Melolonthidae, Scarabaeidae y Passalidae, entre las qu...

  16. A new species of Oxelytrum Gistel (Coleoptera, Silphidae) from southern Argentina, with a key to the species of the genus

    Science.gov (United States)

    Oliva, Adriana

    2012-01-01

    Abstract A new species of the forensically interesting genus Oxelytrum Gistel (Coleoptera, Silphidae), Oxelytrum selknan, is described from Santa Cruz and Tierra del Fuego provinces, Argentina. The new species resembles Oxelytrum biguttatum (Philippi) in outer aspect, but has different male genitalia, in particular a median lobe longer than the paramera. All the described species of Oxelytrum have the median lobe shorter than the paramera. The internal sac, as far as it could be reconstructed from dry-pinned specimens, also shows differences between the two species. A key to the species of Oxelytrum is given and illustrated. PMID:22773910

  17. Erotylidae (Insecta, Coleoptera of Poland – problematic taxa, updated keys and new records

    Directory of Open Access Journals (Sweden)

    Rafal Ruta

    2011-10-01

    Full Text Available New data concerning the occurrence of pleasing fungus beetles (Coleoptera: Erotylidae in Poland are given, with a focus on rare and difficult to identify Central European taxa. Cryptophilus cf. integer (Heer (Cryptophilinae is reported from the Polish territory for the first time based on adult and larval specimens collected in the Wielkopolska-Kujawy Lowland. Identification problems concerning species of Cryptophilus introduced to Europe are discussed. Triplax carpathica Reitter (Erotylinae is recorded from the Białowieża Primeval Forest, which is the first known non-Carpathian finding of this species, located in the close proximity of the Polish-Belarussian UNESCO World Heritage Site “Białowieża Forest”. Discussion of T. carpathica being conspecific with Siberian T. rufiventris Gebler is provided. New Polish localities of several other Erotylidae are reported, and an updated key to Central European species of Triplax is given. The Triplax key is supplemented with dorsal and ventral habitus images of all treated Triplax species. One of the rarest Central European erotyline species Combocerus glaber (Schaller is recorded from xerothermic grasslands in North-West Poland.

  18. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

    International Nuclear Information System (INIS)

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

  19. The complete mitochondrial genome of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Qiu-Ning; Bian, Dan-Dan; Jiang, Sen-Hao; Li, Zhen-Xing; Ge, Bao-Ming; Xuan, Fu-Jun; Yang, Li; Li, Chao-Feng; Zhang, Dai-Zhen; Zhou, Chun-Lin; Tang, Bo-Ping

    2016-01-01

    The complete mitochondrial genome (mitogenome) of Tribolium castaneum (Coleoptera: Tenebrionidae) was determined to be 15,883 bp (GenBank accession No. KM009121), which contains 22 tRNA genes, 13 protein-coding genes (PCGs), 2 rRNA genes and a major non-coding A + T-rich region. It has the typical gene organization and order of mitogenomes from ancestral insects. The nucleotide composition was also biased toward A + T nucleotides (71.72%) and the AT skew of this mitogenome was slightly positive. All of the 22 tRNA genes displayed a typical clover-leaf structure, with the exception of trnS1 (AGN). Thirteen PCGs were initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene which was initiated by AAT. Eight of the 13 PCGs harbor the incomplete termination codon by T or TA. The A + T-rich region of the mitogenome was 1237 bp in length and the A + T content was 82.30%. PMID:25162515

  20. Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae

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    Jerez, V.

    2011-12-01

    Full Text Available We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini. Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value.

  1. Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera).

    Science.gov (United States)

    Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sárah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cássia

    2008-12-01

    The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n=20 and a parachute type sex determining system and the karyotype was 2n=20,Xy in two species and 2n=18,Xy(p), 2n=19,X0, 2n=12,XY and 2n=14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed, whereas the y chromosome presented two arms or was punctiform. In conclusion, the subfamily Scarabaeinae comprises 120 species analyzed cytogenetically, and are observed the occurrence of five chromosome rearrangements (autosome-autosome and X-autosome fusions, pericentric inversions, fissions and loss of the y chromosome) that are related to the chromosome variability and evolution in the group. PMID:18495484

  2. Fruit Damage Patterns Caused by Ovipositing Females of Conotrachelus dimidiatus (Coleoptera: Curculionidae in Guava Trees

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    Felipe Tafoya

    2010-01-01

    Full Text Available We evaluated the damage patterns produced by females of the guava weevil Conotrachelus dimidiatus Champion, 1904 (Coleoptera: Curculionidae, according to the position of the damaged fruit in guava trees Psidium guajava L. in Calvillo, Aguascalientes, Mexico. The trees were subdivided in eight zones, and during one year the level of fruit lesions due to oviposition was registered. Results showed a higher level of damage in the upper and external zone of the trees (P≤.05. We found no significant differences in damage between the four cardinal points (P≥.05. During the year, the level of damage was recorded and was higher in the months of August and September (P≤.05 associated with rainfall (0.86, P=.06 and increase in temperature (0.84, P=.03. The most susceptible fruits were in the size range of 2.1–4.0 cm (polar diameter. The information from this study will be used to design and establish effective control strategies for the guava weevil, taking into account location of the most susceptible fruits, seasonality of the pest, and the abiotic factors.

  3. The Hydraulic Mechanism of the Unfolding of Hind Wings in Dorcus titanus platymelus (Order: Coleoptera

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    Jiyu Sun

    2014-04-01

    Full Text Available In most beetles, the hind wings are thin and fragile; when at rest, they are held over the back of the beetle. When the hind wing unfolds, it provides the necessary aerodynamic forces for flight. In this paper, we investigate the hydraulic mechanism of the unfolding process of the hind wings in Dorcus titanus platymelus (Oder: Coleoptera. The wing unfolding process of Dorcus titanus platymelus was examined using high speed camera sequences (400 frames/s, and the hydraulic pressure in the veins was measured with a biological pressure sensor and dynamic signal acquisition and analysis (DSA during the expansion process. We found that the total time for the release of hydraulic pressure during wing folding is longer than the time required for unfolding. The pressure is proportional to the length of the wings and the body mass of the beetle. A retinal camera was used to investigate the fluid direction. We found that the peak pressures correspond to two main cross-folding joint expansions in the hind wing. These observations strongly suggest that blood pressure facilitates the extension of hind wings during unfolding.

  4. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae

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    Andréa Roveré Franz

    2011-03-01

    Full Text Available Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus (Coleoptera, Curculionidae. Stored grains are subject to losses in quality nutritional value and in sanitation from the time they are stored to the time they are consumed. Botanical insecticides may offer an alternative solution for pest control. The objective was to test the insecticidal properties of the essential oils of Cymbopogon citratus (leaf, Zingiber officinale (root and Mentha sp. (leaf. The efficacy of these oils was tested to control the rice weevil, S. oryzae, using hydrodistillation. Chemical analysis of the essential oils was carried out by gas chromatography. Major components of C. citratus were geranial (48% and neral (31%, of Z. officinale were α-zingibereno (13%, geranial (16%, neral (10% and α-farneseno (5% and of Mentha sp. was menthol (92%. Bioassays were carried out by fumigation and topical application. In topical application assays, the essential oil of C. citratus had greater toxicity (LC50 0.027 µL mL-1 and shorter exposure time than the oils of the other two plants. After 24 h and 48 h, 70% and 100% mortality of S. oryzae occurred, respectively. In fumigation assays, essential oil of Z. officinale had a lower LC50 (1.18 µL cm-2 and 70% mortality after 24 h exposure. Therefore, we recommend the use of essential oils of C. citratus and Z. officinale to control the rice weevil S. oryzae.

  5. On the spatial spread of the Rice Water Weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Erirhinidae, in Italy

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    Daniela Lupi

    2010-07-01

    Full Text Available A five year study has been made to establish the spread of the rice water weevil Lissorhoptrus oryzophilus (Coleoptera: Erirhinidae in Northern Italy. Data obtained with GPS from 2005 throughout 2009 were first georeferenced with SW ArcGis® 9.2, then overlapped and compared to the map of the European environmental landscape based on the interpretation of satellite images (CORINE Land Cover map and to the hydrographic chart CT10 (Technical Regional map 10000. The analysis of the radial rate of spread per year indicates a deceleration in the expansion from 10.864 ± 6.801 km/year in 2005 to 5.318 ± 1.401 km/year in 2009. In five years the weevil has expanded its distribution in nearly all rice paddies in Lombardy and Piedmont, over an area of about 200,000 ha, which correspond to 86% of the total Italian rice area. Its expansion is thought to follow a type of stratified dispersal, due both to insect adult active dispersal and to accidental movements caused by human transportation.

  6. Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae in Bulgaria

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    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bark beetles (Coleoptera: Curculionidae: Scolytinae belong to one of the most damaging groups of forest insects and the activity of their natural enemies –pathogens, parasitoids,parasites or predators suppressing their population density,is of great importance. Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated sporadically. The aim of this preliminary study was to find, identify and study morphological characteristics of fungal entomopathogens naturally-occurring in populations of three curculionid species – Ips sexdentatus Boern, Ips typographus (L. and Dryocoetes autographus (Ratz.. Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collectedfrom forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract and were identified based on morphological characteristics both on the host and in a culture.Morphological characteristics of the isolates were studied by phenotypic methods. The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D. autographus were found to belong to the species Beauveria bassiana (Bals. – Criv. Vuillemin,Beauveria brongniartii (Saccardo Petch and Isaria farinosa (Holmsk. Fries (anamorph Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae. Morphological traits of the isolates are described.

  7. Pimenta pseudocaryophyllus Derivatives: Extraction Methods and Bioactivity Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Ansante, T F; Niculau, E S; Pavarini, R; Silva, M F G F; Seffrin, R C; Vendramim, J D

    2015-12-01

    Plant-based insecticides can play an important role in integrated insect pest management (IPM), especially in protecting stored grains. The aim of this study was to evaluate the bioactivity of derivatives (powder, ethanolic extract, and essential oil (EO)) from the leaves of Pimenta pseudocaryophyllus (Myrtaceae), a Brazilian native species, against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), the main insect pest of stored corn. The powder and essential oil prepared from leaves showed a repellent effect. Moreover, the EO exhibited promising insecticidal activity through residual contact (LC50 = 1522 mg kg(-1)) and significantly decreased the F 1 progeny and the percentage of damaged grains. However, the essential oil obtained from P. pseudocaryophyllus leaves did not result in significant mortality of S. zeamais adults after 72 h of exposure by fumigation in concentrations up to 400 μL L(-1) of air. Based on GC-MS analysis, 20 compounds were identified in the essential oil of P. pseudocaryophyllus leaves, being chavibetol (38.14%), methyl eugenol (11.35%), and terpinolene (9.17%) as the major constituents. Essential oil from P. pseudocaryophyllus leaves is an interesting source of compounds with grain-protectant properties and should be analyzed in future studies aiming to develop new bioinsecticides to use in the IPM of stored grains. PMID:26285912

  8. Coexistence and Competition between Tomicus yunnanensis and T. minor (Coleoptera: Scolytinae in Yunnan Pine

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    Rong Chun Lu

    2012-01-01

    Full Text Available Competition and cooperation between bark beetles, Tomicus yunnanensis Kirkendall and Faccoli and Tomicus minor (Hartig (Coleoptera: Scolytinae were examined when they coexisted together in living Yunnan pine trees (Pinus yunnanensis Franchet in Yunnan province in Southwest China. T. yunnanensis bark beetles were observed to initiate dispersal from pine shoots to trunks in November, while the majority of T. minor begins to transfer in December. T. yunnanensis mainly attacks the top and middle parts of the trunk, whereas T. minor mainly resides in the lower and middle parts of the trunk. The patterns of attack densities of these two species were similar, but with T. yunnanensis colonizing the upper section of the trunk and T. minor the lower trunk. The highest attack density of T. Yunnanensis was 297 egg galleries/m2, and the highest attack density of T. minor was 305 egg galleries/m2. Although there was significant overlap for the same bark areas, the two species generally colonize different areas of the tree, which reduces the intensity of competition for the relatively thin layer of phloem-cambium tissues where the beetles feed and reside.

  9. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae and Collection Efficiency

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    Fred J. Eller

    2014-11-01

    Full Text Available Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q, male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about age 15 days old and then tapered off. Male pepper weevils produced the highest amount of pheromone between noon and 2 pm (i.e., 4 to 6 h after “lights on” and were producing ca. 800 ng/h during this period. Thereafter, pheromone production decreased and was extremely low during the scotophase (i.e., ca. 12 ng/h. Male pepper weevil density had a significant effect on both release rate and pheromone composition. Pheromone production on a per male basis was highest for individual males and the percentage of geranic acid in the blend was lowest for individual males. Male pepper weevils produced only extremely low amounts of pheromone when feeding on artificial diet; however, they produced very high amounts when on fresh peppers. Together, this information will be useful in designing better attractant lures for pepper weevils.

  10. Insecticidal Efficacy of Some Lamiaceae Plant Extracts Against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae

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    M.M. Kiradoo

    2011-03-01

    Full Text Available The present study was undertaken to screen some plants belonging to family Lamiaceae against Callosobruchus chinensis Linn.. Among fourteen important insect pests of stored grains, the pulse beetle C. chinensis (Coleoptera: Bruchidae is one such pest causing considerable damage to stored pulses. The eggs are laid on the host grains; the larvae bore inside and after feeding and pupating emerge out as adults leaving behind damaged hollow seed-grains. Looking into the hazards of chemical insecticides, there is an utmost need to search for some alternate methods for protection against the damage caused by insects. The leaves of the select plants viz., Ocimum basilicum, O. sanctum and Mentha spicata were employed in the form of various formulations and mortality of the pest insect was assessed. A significant increase in mortality of C. chinensis was observed in all the experimental sets as compared to normal and control sets during the present study. When comparisons were made on the basis of ANOVA to compare the effect of the three plants on mortality, it was found that the treatments of O. basilicum were superior over M. spicata and O. sanctum. The findings overall suggest that all the three plants screened have a potential to be used against the pest C. chinensis and can be employed as an alternate to chemical insecticides in household and storehouses to minimize the infestation and damage caused by the bruchid.

  11. Preliminary Diversity Of Dung Beetles (Coleoptera: Scarabaeinae Attracting By Human Dung (Calvario, Meta

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    Ruth Mariela Castillo Morales

    2011-12-01

    Full Text Available In the Private Reserve, Refugio del Oso de Anteojos (El Calvario-Meta, the diversity of dung beetles was studied (Coleoptera: Scarabaeinae, grouped around different types of vegetation (Montane Rain Forest, Rainforest Premontane, Lower Montane Rain Forest, by using pitfall traps baited with human dung. During the samplings carried out in the rainy station on 2008, were captured a total of 177 individuals belonging to 9 species. The tribe Canthonini was the most representative with 87.5%, of the described species, followed by Aechini 10.8%, and Onthophagini 1.7%. The dominant species was Canthon lituratus in the three vegetation units: Lower montane rain forest was the richest (9 species and abundance (86 individuals, compared with other environments sampled. By calculating the complementarity index, shows a similar species composition in the three habitats sampled, possibly, related to the floristic composition presented in each one of them.

  12. Characterization of the complete mitochondrial genome of the firefly, Luciola substriata (Coleoptera: Lampyridae).

    Science.gov (United States)

    Mu, Feng-Juan; Ao, Liang; Zhao, Hua-Bin; Wang, Kai

    2016-09-01

    The firefly, Luciola substriata (Coleoptera: Lampyridae), is an aquatic firefly species, whose larvae inhabit ponds or lakes. Here we present the complete mitochondrial (mt) genome of the firefly (GenBank accession number KP313820) and provide its annotation. This circular genome is 16,248 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding AT-rich region. Similar to other firefly species, the base composition of this mitochondrial genome is also biased toward A and T (44.09% A, 34.00% T, 12.89% C, and 9.01% G). All 13 protein-coding genes start with a typical mitochondrial start codon, and terminate with a usual stop codon TAA, or TAG or a single T. The non-coding AT-rich region (1636 bp in length) include one (A)20, and two (T)15 tandem repeats, and one (AAT)5 element. This mitochondrial genome sequence will promote a better understanding for firefly evolution in the future. PMID:25714154

  13. Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    Liu, Su; Rao, Xiang-Jun; Li, Mao-Ye; Feng, Ming-Feng; He, Meng-Zhu; Li, Shi-Guang

    2015-03-01

    We present the first antennal transcriptome sequencing information for the yellow mealworm beetle, Tenebrio molitor (Coleoptera: Tenebrionidae). Analysis of the transcriptome dataset obtained 52,216,616 clean reads, from which 35,363 unigenes were assembled. Of these, 18,820 unigenes showed significant similarity (E-value SNMP) genes. BLASTX best hit results indicated that these chemosensory genes were most identical to their respective orthologs from Tribolium castaneum. Phylogenetic analyses also revealed that the T. molitor OBPs and CSPs are closely related to those of T. castaneum. Real-time quantitative PCR assays showed that eight TmolOBP genes were antennae-specific. Of these, TmolOBP5, TmolOBP7 and TmolOBP16 were found to be predominantly expressed in male antennae, while TmolOBP17 was expressed mainly in the legs of males. Several other genes were identified that were neither tissue-specific nor sex-specific. These results establish a firm foundation for future studies of the chemosensory genes in T. molitor. PMID:25665775

  14. Observations on the Cave-Associated Beetles (Coleoptera of Nova Scotia, Canada

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    Moseley M.

    2009-07-01

    Full Text Available The cave-associated invertebrates of Nova Scotia constitute a fauna at a very early stage of post-glacial recolonization. TheColeoptera are characterized by low species diversity. A staphylinid Quedius spelaeus spelaeus, a predator, is the only regularlyencountered beetle. Ten other terrestrial species registered from cave environments in the province are collected infrequently. Theyinclude three other rove-beetles: Brathinus nitidus, Gennadota canadensis and Atheta annexa. The latter two together with Catopsgratiosus (Leiodidae constitute a small group of cave-associated beetles found in decompositional situations. Quedius s. spelaeusand a small suite of other guanophiles live in accumulations of porcupine dung: Agolinus leopardus (Scarabaeidae, Corticariaserrata (Latrididae, and Acrotrichis castanea (Ptilidae. Two adventive weevils Otiorhynchus ligneus and Barypeithes pellucidus(Curculionidae collected in shallow cave passages are seasonal transients; Dermestes lardarius (Dermestidae, recorded fromone cave, was probably an accidental (stray. Five of the terrestrial beetles are adventive Palaearctic species. Aquatic beetles arecollected infrequently. Four taxa have been recorded: Agabus larsoni (Dytiscidae may be habitual in regional caves; another Agabussp. (probably semivittatus, Dytiscus sp. (Dytiscidae, and Crenitis digesta (Hydrophilidae are accidentals. The distribution andecology of recorded species are discussed, and attention is drawn to the association of beetles found in a Nova Scotia “ice cave”.

  15. Population dynamics and spatial distribution of Abaris basistriata Chaudoir, 1873 (Coleoptera: Carabidae

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    Ivan Carlos Fernandes Martins

    2016-02-01

    Full Text Available ABSTRACT Abaris basistriata, a beetle species dominant in agroecosystems and natural habitats, may benefit from the establishment of nearby refuge areas or crop field centers. To confirm this hypothesis, we analyzed the spatial distribution of the species and verified the population dynamics of this predator in a soybean/corn rotation crop and a central refuge area. The 1-ha experimental area was divided in half by a range of herbaceous plants (2 m in width and 80 m in length. Beetle samples were collected using pitfall traps every fortnight during the in-season and every month during the off-season (a total of 27 sampling occurrences. Population fluctuation was analyzed by correlating the total number of specimens with plant phenology. We used multiple regression analysis with variable (stepwise selection to examine the influence of meteorological factors on species occurrence. To determine the spatial distribution, data were analyzed using dispersion indices and probabilistic models based on the Coleoptera frequency distribution. Distribution visualization was assessed using a linear interpolation map. A total of 143 A. basistriata specimens were collected, with 83 from the soybean/corn area and 60 from the refuge area. Periods of large population size occurred during a season with high rainfall and high maximum and minimum temperatures. On the basis of the spatial distribution analysis of A. basistriata, it is likely that the beetles occur in an aggregate form, preferably in the refuge area.

  16. Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil

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    Andreia Mauruto Chernaki-Leffer

    2011-03-01

    Full Text Available Susceptibility of Alphitobius diaperinus (Panzer (Coleoptera, Tenebrionidae to cypermethrin, dichlorvos and triflumuron in southern Brazil. The lesser mealworm, Alphitobius diaperinus (Panzer, is an important insect pest in poultry houses in Brazil. Susceptibility of the lesser mealworm collected from eight poultry houses in Paraná state, southern Brazil, was evaluated for cypermethrin, dichlorvos and triflumuron. Adult A. diaperinus were tested in bioassays with cypermethrin and dichlorvos. Larvae were fed rabbit feed wetted with a triflumuron-water solution. Concentration-mortality regressions were estimated using Probit analysis and resistance ratios were calculated based on the susceptible population. Among the field populations evaluated, cypermethrin LC50 values for adults, ranged from 68.1 to 6,263 ng (AI/cm². LC50 values for adults challenged with dichlorvos ranged from 10.3 to 1,385 ng (AI/cm². One population from Pato Branco showed reduced susceptibility to triflumuron (LC50 = 272 µg (AI/ml of solution when compared to the most susceptible population (LC50 = 109.8 µg (AI/ml. Application of cypermethrin and dichlorvos analogues should be managed with caution to minimize insecticide resistance problems.

  17. Pitfall Traps and Mini-Winkler Extractor as Complementary Methods to Sample Soil Coleoptera.

    Science.gov (United States)

    Carneiro, A C; Batistella, D A; Battirola, L D; Marques, M I

    2016-02-01

    We compared abundance, species richness, and capture efficiency with pitfall traps and mini-Winkler extractors to examine their use as complementary methods for sampling soil Coleoptera during dry (2010) and high water seasons (2011) in three areas, including inundated and non-inundated regions, in the Pantanal of Poconé, Mato Grosso, Brazil. We paired treatments with two 10 × 10 m plots in inundated and non-inundated locations that were repeated three times in each location for a total of 18 plots. In each plot, we used nine pitfall traps and collected 2 m(2) of leaf litter and surface soil samples with mini-Winkler extractors. We collected a total of 4260 adult beetles comprising 36 families, 113 genera, and 505 species. Most were caught in pitfalls (69%) and the remainder in the mini-Winkler extractors (31%). Each method provided distinct information about the beetle community: 252 species were captured only in pitfall traps, 147 using only the mini-Winkler extractors, and these methods shared another 106 species. Pitfall and mini-Winkler contribute in different ways for the sampling of the soil beetle community, and so they should be considered complementary for a more thorough assessment of community diversity. PMID:26493175

  18. Oil pipeline corridor through an intact forest alters ground beetle (Coleoptera: Carabidae) assemblages in southeastern Ohio.

    Science.gov (United States)

    Silverman, Bareena; Horn, David J; Purrington, Foster F; Gandhi, Kamal J K

    2008-06-01

    Litter-dwelling ground beetle (Coleoptera: Carabidae) assemblages were monitored 1 yr after the construction of a corridor for installation of an oil pipeline along a xeric ridge-top forest in southeastern Ohio. After the creation of the corridor, three distinct habitats were evident in these sites: open corridor, ecotone areas around the corridor, and undisturbed forest interior. Carabidae were collected using directional pitfall traps that were placed parallel and perpendicular to the corridor in each of the three habitats. Results indicate that more carabids were present in the ecotone than in the other two habitats. Carabid diversity as estimated by rarefaction was highest in the corridor followed by ecotone and forest interior. Generalist and forest specialists such as Synuchus impunctatus (Say), Carabus goryi Dejean, and Pterostichus trinarius (Casey) were present in greater numbers in the forest interior and ecotone assemblages. In contrast, open-habitat specialists such as Harpalus pensylvanicus (DeGeer) and Selenophorus opalinus (LeConte) were present in greater numbers in the corridor assemblages. Carabid assemblages of the corridor were distinct from those of the ecotone and forest interior, whereas the latter two habitats had very similar assemblages. The successional pathway of the corridor carabid assemblage will therefore be likely different from that of the forest interior and ecotone. Overall, results indicate that construction of the oil pipeline corridor had significant short-term effects on the carabid numbers, diversity, and species composition because of ensuing habitat changes and fragmentation of the forest. PMID:18559178

  19. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae)

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms.

  20. Demographic comparison and population projection of Rhynchophorus ferrugineus (Coleoptera: Curculionidae) reared on sugarcane at different temperatures.

    Science.gov (United States)

    Peng, Lu; Miao, Yunxin; Hou, Youming

    2016-01-01

    Understanding how temperature affects fitness is important for conservation and pest management, especially in the era of global climate change. Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae) is a worldwide pest of many economically important crops. Although much is known about this pest's life cycle, its adaptability to different temperatures is not fully understood. Here, we used age- and stage-specific life tables to investigate the effects of temperature on fitness-related traits and demographic parameters of R. ferrugineus under eight constant temperature regimens in the laboratory. The growth potential of these populations was also evaluated. The greatest longevity for males and females was 158.0 d at 24 °C and 144.5 d at 21 °C, respectively, but mean total fecundity was the highest at 27 °C. The intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0) increased initially at low temperatures and then decreased. All metrics reached a maximum at 27 °C and a minimum at 36 °C. Mean generation times (T ) decreased across the temperature range with a minimum at 36 °C. Our results indicate that the optimum temperature for growth of R. ferrugineus was approximately 27 °C. Our work will be of value for developing strategies for control management of this pest species. PMID:27545594

  1. Notas e novas espécies de Onciderini (Coleoptera, Cerambycidae, Lamiinae

    Directory of Open Access Journals (Sweden)

    Ubirajara R. Martins

    2007-01-01

    Full Text Available Novas espécies descritas do Brasil: Hypsioma carioca sp. nov. (Rio de Janeiro e Hesychotypa maraba sp. nov. (Pará; do Equador: Sternycha ecuatoriana sp. nov. (Pichincha; da Bolívia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. e Alexera secunda sp. nov. Transfere-se Hesycha strandi (Breuning, 1943 para o gênero Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 é considerada sinônima de H. miniata Thomson, 1868. Novo registro (Trinidad e figura são dados para Trachysomus surdus.Notes and new species of Onciderini (Coleoptera, Cerambycidae, Lamiinae. New species described from Brazil: Hypsioma carioca sp. nov. (Rio de Janeiro and Hesychotypa maraba sp. nov. (Pará; from Ecuador: Sternycha ecuatoriana sp. nov. (Pichincha; from Bolivia (Santa Cruz: Hesychotypa magnifica sp. nov., Tibiosioma maculosa sp. nov. and Alexera secunda sp. nov. Hesycha strandi (Breuning, 1943 is transferred to the genus Cacostola Fairmaire & Germain, 1859. Hesychotypa archippa Dillon & Dillon, 1946 is considered a synonym of H. miniata Thomson, 1868. New record (Trinidad and figure for Trachysomus surdus Dillon & Dillon, 1946 are given.

  2. Stenusine, an antimicrobial agent in the rove beetle genus Stenus (Coleoptera, Staphylinidae).

    Science.gov (United States)

    Lusebrink, Inka; Dettner, Konrad; Seifert, Karlheinz

    2008-08-01

    Stenusine is well known as the alkaloid, discharged by the rove beetle, genus Stenus Latreille (Coleoptera, Staphylinidae). The Stenus beetles employ the alkaloid as an escape mechanism when on water surfaces. In the case of danger, they lower their abdomen and emit stenusine from their pygidial glands. Stenusine shows a low surface tension and therefore a high spreading pressure; these properties propel the beetle quickly over the water. Many Steninae do not live in habitats with open waters, but in detritus, leaf litter, mosses, etc. This raises the possibility that stenusine might also have another function, e.g., as antibiotic or fungicide. Stenus beetles show an intense grooming behaviour. With gas chromatography-mass spectrometry analyses we could prove that they cover themselves with their secretion. To tests its antimicrobial properties we conducted agar diffusion tests with stenusine and norstenusine, another substance that is abundant in most Stenus species. Both compounds have an antimicrobial effect on entomopathogenic bacteria and fungi. Stenusine not only allows for an extraordinary method of locomotion on water surfaces, it also protects the Steninae from being infested with microorganisms. PMID:18392795

  3. A multilayer micromechanical model of the cuticle of Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae).

    Science.gov (United States)

    Andrew Jansen, M; Singh, Sudhanshu S; Chawla, Nikhilesh; Franz, Nico M

    2016-08-01

    Curculio longinasus Chittenden, 1927 (Coleoptera: Curculionidae), is a weevil species common throughout the southwestern United States that uses its rostrum - a very slender, curved, beak-like projection of the head - to excavate tunnels in plant organs (such as acorns) for egg laying (oviposition). Once the apical portion of the rostrum has been inserted into the preferred substrate for oviposition, the female begins rotating around the perimeter of the hole, elevating her head by extending the fore-legs, and rotating the head in place in a drilling motion. This action causes significant elastic deformation of the rostrum, which will bend until it becomes completely straight. To better understand the mechanical behavior of the cuticle as it undergoes deformation during the preparation of oviposition sites, we develop a comprehensive micro/macro model of the micromechanical structure and properties of the cuticle, spanning across all cuticular regions, and reliably mirroring the resultant macroscale properties of the cuticle. Our modeling approach relies on the use of multi-scale, hierarchical biomaterial representation, and employs various micromechanical schemata - e.g., Mori-Tanaka, effective field, and Maxwell - to calculate the homogenized properties of representative volume elements at each level in the hierarchy. We describe the configuration and behavior of this model in detail, and discuss the theoretical implications and limitations of this approach with emphasis on future biomechanical and comparative evolutionary research. Our detailed account of this approach can thereby serve as a methodological template for exploring the biomechanical behavior of new insect structures. PMID:27189867

  4. Transcriptomic Analysis of Eucryptorrhynchus chinensis (Coleoptera: Curculionidae) Using 454 Pyrosequencing Technology

    Science.gov (United States)

    Liu, Zhen-Kai; Wen, Jun-Bao

    2016-01-01

    Eucryptorrhynchus chinensis Olivier (Coleoptera: Curculionidae) is one of the most important pests of Ailanthus altissima; however, so far, no studies on the genome or transcriptome of E. chinensis have been reported. Using the Roche 454 FLX Titanium platform, an RNA pool obtained from E. chinensis eggs, larva, pupae, and adults was sequenced and assembled de novo to achieve maximum diversity of sampled transcripts. We obtained 1,441,137 (∼518 Mb) raw reads with an average length of 360 bp. After trimming, 89% qualified reads were produced and assembled into 35,509 isotigs with an average length of 440 bp, N50 of 1,048 bp, and 111,643 singletons. We generated 87,894 unigenes following a cluster analysis of the isotigs and singletons, and then functionally annotated the unigenes with gene descriptions. We obtained 23,363 GO assignments, and 12,724 unigenes were assigned to KOG. Based on these annotations, 294 biochemical pathways involved in growth, reproduction, and stress or immune responses were predicted. A total of 659,026 single nucleotide variants and 6,112 simple sequence repeats were detected. Our data provide comprehensive information on the sequence and possible functions of E. chinensis transcripts.

  5. Endemic epigean Tenebrionids (Coleoptera: Tenebrionidae) from the Andean Region: exploring the patagonian-diversification hypothesis.

    Science.gov (United States)

    Carrara, Rodolfo; Flores, Gustavo E

    2015-01-01

    Tenebrionidae is a diverse insect family of Coleoptera that shows high levels of endemicity in epigean species. For the Andean region, which is divided into three subregions: Central Chilean, Subantarctic and Patagonian, it has been hypothesized that epigean tenebrionids have diversified in the Patagonian subregion and subsequently, they dispersed to Subantarctic and Central Chilean subregions. In this work, based on information obtained from museum collections and scientific studies, we presented the first list of endemic epigean tenebrionids from the Andean region with their taxonomic arrangement and geographic distribution. Moreover, we used these data to explore the veracity of the Patagonian-diversification hypothesis. A total of 416 species grouped into six subfamilies, 17 tribes and 41 genera were identified as endemic to the Andean region. Considering the spatial distribution it was observed that subfamilies, tribes, genera and species were unequally distributed across subregions. Results did not support the Patagonian-diversification hypothesis; to the contrary, they were more concordant with processes of isolation among subregions that have promoted speciation by interrupting gene flow among populations, resulting in endemism because species can not expand their range sizes. Finally, we discuss the implications of our findings to be considered in biodiversity conservation, because endemic species, by their high extinction risk, are primary targets in conservation strategies. PMID:26623788

  6. Pathology of Yersinia entomophaga MH96 towards Costelytra zealandica (Coleoptera; Scarabaeidae) larvae.

    Science.gov (United States)

    Hurst, Mark Robin Holmes; van Koten, Chikako; Jackson, Trevor Anthony

    2014-01-01

    The bacterium Yersinia entomophaga was isolated from larvae of the New Zealand grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), found in soil. Following ingestion of a lethal dose of bacteria, larvae of C. zealandica reduced feeding activity and movement. After approximately 4h infected larvae convulsed and regurgitated dark digestive fluid and expelled frass pellets leaving the midgut empty and the larva amber in appearance. In the initial stages of infection, ingested bacteria were mostly contained within the peritrophic membrane and expelled with the gut fluid or transferred into the hind gut. While few Y. entomophaga were associated with the midgut epithelial cells, by 24h cells were swelling and bursting with vesicles being expelled into the midgut lumen. By 48h, bacteria had entered the haemocoel and the midgut cells had further deteriorated. After 72h, the cellular remnants were totally detached from the basal membrane the infected insects were filled with bacteria and moribund or dead with septicaemia. Mortality was directly proportional to dose and time after infection. By applying a range of doses, the LD50 was determined as 2.9×10(4)Y. entomophaga per C. zealandica larva, with an LT50 of 2.94days for doses of>1×10(5) per larva. Ingestion of low doses of bacteria did not inhibit feeding activity but led more slowly to death. By time of death, Y. entomophaga had multiplied, approximately 500 fold, in the cadavers of the infected larvae. PMID:24291403

  7. Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

    Science.gov (United States)

    Downey, Danielle; Chun, Stacey; Follett, Peter

    2015-06-01

    Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. PMID:26470205

  8. Ambrosia Beetle (Coleoptera: Scolytidae) Species, Flight, and Attack on Living Eastern Cottonwood Trees.

    Energy Technology Data Exchange (ETDEWEB)

    Coyle, D R; D.C. Booth: M.S. Wallace

    2005-12-01

    ABSTRACT In spring 2002, ambrosia beetles (Coleoptera: Scolytidae) infested an intensively managed 22-ha tree plantation on the upper coastal plain of South Carolina. Nearly 3,500 scolytids representing 28 species were captured in ethanol-baited traps from 18 June 2002 to 18 April 2004. More than 88% of total captures were exotic species. Five species [Dryoxylon onoharaensum (Murayama), Euwallacea validus (Eichhoff), Pseudopityophthorus minutissimus (Zimmermann), Xyleborus atratus Eichhoff, and Xyleborus impressus Eichhoff]) were collected in South Carolina for the first time. Of four tree species in the plantation, eastern cottonwood, Populus deltoides Bartram, was the only one attacked, with nearly 40% of the trees sustaining ambrosia beetle damage. Clone ST66 sustained more damage than clone S7C15. ST66 trees receiving fertilization were attacked more frequently than trees receiving irrigation, irrigation_fertilization, or controls, although the number of S7C15 trees attacked did not differ among treatments. The study location is near major shipping ports; our results demonstrate the necessity for intensive monitoring programs to determine the arrival, spread, ecology, and impact of exotic scolytids.

  9. Evolution of subterranean diving beetles (Coleoptera: Dytiscidae: Hydroporini, Bidessini) in the arid zone of Australia.

    Science.gov (United States)

    Leys, Remko; Watts, Chris H S; Cooper, Steve J B; Humphreys, William F

    2003-12-01

    Calcrete aquifers in arid inland Australia have recently been found to contain the world's most diverse assemblage of subterranean diving beetles (Coleoptera: Dytiscidae). In this study we test whether the adaptive shift hypothesis (ASH) or the climatic relict hypothesis (CRH) is the most likely mode of evolution for the Australian subterranean diving beetles by using a phylogeny based on two sequenced fragments of mitochondrial genes (CO1 and 16S-tRNA-ND1) and linearized using a relaxed molecular clock method. Most individual calcrete aquifers contain an assemblage of diving beetle species of distantly related lineages and/or a single pair of sister species that significantly differ in size and morphology. Evolutionary transitions from surface to subterranean life took place in a relatively small time frame between nine and four million years ago. Most of the variation in divergence times of the sympatric sister species is explained by the variation in latitude of the localities, which correlates with the onset of aridity from the north to the south and with an aridity maximum in the Early Pliocene (five mya). We conclude that individual calcrete aquifers were colonized by several distantly related diving beetle lineages. Several lines of evidence from molecular clock analyses support the CRH, indicating that all evolutionary transitions took place during the Late Miocene and Early Pliocene as a result of aridification. PMID:14761060

  10. Morfología del tracto digestivo y sistema reproductor femenino de Compsus sp. (Coleoptera: Curculionidae Morphology of the digestive tract and female reproductive system of Compsus sp. (Coleoptera: Curculionidae

    Directory of Open Access Journals (Sweden)

    JOSÉ DAVID RUBIO G

    2007-12-01

    Full Text Available Se realizó el estudio morfológico del tracto digestivo y sistema reproductor femenino del picudo de los cítricos, Compsus sp. (Coleoptera: Curculionidae. El tracto digestivo presentó un arreglo típico descrito para otros insectos. Se encontraron diferencias morfológicas, sin embargo, en el buche en donde se observan unas estructuras espinosas. A nivel del mesenterón, se observó que el ventrículo anterior se encuentra dividido en sacos de tamaño medio y en el ventrículo posterior se proyectan numerosos ciegos gástricos. El sistema reproductor de la hembra mostró un arreglo estructural común a especímenes de la familia Curculionidae.A morphological examination was conducted on the digestive tract and the female reproductive system of the citrus weevil, Compsus sp. (Coleoptera: Curculionidae. The digestive tract of Compsus sp. showed a typical arrangement as described for other insects. Morphological differences, however, were found in the crop of the foregut, which exhibited several spine-like structures. At the midgut level, the anterior ventricle was divided into medium-size sacs and numerous gastric caecae projected into the posterior ventricle. The female reproductive system showed a structural arrangement common to individuals of the family Curculionidae.

  11. Cowpea Vicilins: Fractionation of Urea Denatured Sub-Units and Effects on Callosobruchus maculatus F. (Coleoptera: Bruchidae Development

    Directory of Open Access Journals (Sweden)

    Antônio Chagas Mota

    2002-03-01

    Full Text Available Vicilins (7S storage globulins isolated from cowpea (Vigna unguiculata L. seeds which were susceptible (S and resistant (R to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar and resistant (IT81D-1045 line seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.Vicilinas (globulinas de reserva 7S isoladas de sementes de feijão-de-corda (Vigna unguiculata L., susceptíveis (S e resistentes (R ao caruncho/gorgulho (Callosobruchus maculatus F., Coleoptera: Bruchidae foram desnaturadas por uréia e fracionadas por cromatografia de troca iônica. As frações isoladas foram incorporadas em sementes artificiais para avaliação de sua toxicidade a C. maculatus. As fracões mais ácidas de ambas vicilinas afetaram o desenvolvimento e a sobrevivência do bruquídeo. Sugerimos que polipeptídeos de vicilinas de natureza tóxica são expressos em ambos tipos de globulinas de reserva, embora em níveis diferentes.

  12. Typhlocharis vicariantes del Estrecho de Gibraltar. I: Typhlocharis armata Coiffait, 1969 (Coleoptera, Caraboidea, Trechidae

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    Pérez González, S.

    2010-12-01

    Full Text Available This first part about the vicariant Typhlocharis from Gibraltar Strait is dedicated to Typhlocharis armata Coiffait, 1969; a second paper will cover T. silvanoides Dieck, 1869. The studied material was obtained in four field campaigns carried out in the south of Spain and north of Morocco between 1997 and 2001 (Zaballos & Banda, 2000; Zaballos, 2005. In these campaigns abundant specimens of Typhlocharis (Coleoptera, Caraboidea, Trechidae were collected in different localities, the majority of them belonging to the species T. armata Coiffait, 1969. Their study and the comparison with the type series of this species allowed its complete redescription and clarification of its status within the genus. Contrary to what has been considered traditionally, the marginal umbilicate series of elytra in T. armata is composed of three, instead of four posterior setae. The taxonomical affinities of this species are established, the morphological weight of the umbilicate group of elytral setae as a lineage and species group defining character is discussed, and the theory of Jeanne (1973 about the relation between shortening of elytra and umbilicate series reduction with a decrease in body size is revised. The dispersal abilities are evaluated and a biogeographical explanation for the new distribution of the species is proposed.Esta primera parte sobre las especies de Typhlocharis vicariantes del Estrecho de Gibraltar está dedicada a Typhlocharis armata Coiffait, 1969; mientras que en un segundo artículo se tratará T. silvanoides Dieck, 1869. El material estudiado procede de cuatro campañas entomológicas realizadas en el sur de España y en el norte de Marruecos entre 1997 y 2001 (Zaballos & Banda, 2000; Zaballos, 2005. En estas campañas se recolectaron, en localidades diferentes, abundantes ejemplares de Typhlocharis (Coleoptera, Caraboidea, Trechidae, la mayoría de los cuales han resultado pertenecer a la especie T. armata Coiffait, 1969. Su estudio y

  13. Morphological and genetic variation in Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae, Cicindelinae: implications for conservation

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    Serrano, A.

    2003-12-01

    Full Text Available The establishment of taxonomic and geographical boundaries is a common problem when analysing clinal distributions. This is of particular concern when the assessment of intraspecific groupings is required for conservation management. The tiger beetle Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae is a typical case in which two recognised subspecies are distributed in a clinal latitudinal fashion in the dune systems along the Atlantic coast of Portugal. This habitat is increasingly under threat, and conservation measures are needed. We investigated the validity of the two named subspecies, based on a re-analysis of elytral and genitalic measurements using multivariate analysis. We also analysed variation in mitochondrial Cytochrome Oxidase I gene for a total of six populations along the cline. Multivariate analysis supported the idea of a morphological cline and revealed a clear distinction of the southernmost population and also some degree of distinctiveness of the most northern populations, partially supporting the recognised subspecific ranking. The mtDNA analysis identified two main groups corresponding to northern and southern populations. Both sets of markers showed that variation within the C. lusitanica assemblage is complex, with the boundaries between morphological and mtDNA groups not in agreement. However, populations at either end of the distributional range are clearly distinct from each other, and should be considered as provisional units for conservation programmes.El reconocimiento de límites taxonómicos y geográficos de la variabilidad observada es un problema habitual cuando se analizan distribuciones clinales. Esto es particularmente problemático cuando se requiere la determinación de agrupamientos intraespecíficos para tomar medidas de conservación. El cicindélido Cicindela lusitanica Mandl, 1935 (Coleoptera, Carabidae constituye un caso típico en el que dos subespecies reconocidas se hayan distribuidas a

  14. Observations on sex ratio and behavior of males in Xyleborinus saxesenii Ratzeburg (Scolytinae, Coleoptera

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    Peter Biedermann

    2010-09-01

    Full Text Available Strongly female-biased sex ratios are typical for the fungal feeding haplodiploid Xyleborini (Scolytinae, Coleoptera, and are a result of inbreeding and local mate competition (LMC. These ambrosia beetles are hardly ever found outside of trees, and thus male frequency and behavior have not been addressed in any empirical studies to date. In fact, for most species the males remain undescribed. Data on sex ratios and male behavior could, however, provide important insights into the Xyleborini’s mating system and the evolution of inbreeding and LMC in general. In this study, I used in vitro rearing methods to obtain the first observational data on sex ratio, male production, male and female dispersal, and mating behavior in a xyleborine ambrosia beetle. Females of Xyleborinus saxesenii Ratzeburg produced between 0 and 3 sons per brood, and the absence of males was relatively independent of the number of daughters to be fertilized and the maternal brood sex ratio. Both conformed to a strict LMC strategy with a relatively precise and constant number of males. If males were present they eclosed just before the first females dispersed, and stayed in the gallery until all female offspring had matured. They constantly wandered through the gallery system, presumably in search of unfertilized females, and attempted to mate with larvae, other males, and females of all ages. Copulations, however, only occurred with immature females. From galleries with males, nearly all females dispersed fertilized. Only a few left the natal gallery without being fertilized, and subsequently went on to produce large and solely male broods. If broods were male-less, dispersing females always failed to found new galleries.

  15. Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga).

    Science.gov (United States)

    Amaral, Danilo T; Mitani, Yasuo; Ohmiya, Yoshihiro; Viviani, Vadim R

    2016-07-25

    Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a ~1.6kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae+Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal clade within Elateridae. This is the first report that compares the mitochondrial genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. PMID:27060405

  16. The presence of a mycangium in European Sinodendron cylindricum (Coleoptera: Lucanidae) and the associated yeast symbionts

    Science.gov (United States)

    Tanahashi, Masahiko; Hawes, Colin J.

    2016-01-01

    Part of the exoskeleton of some wood-inhabiting insects is modified to form a mycangium, which is a specialized organ used to convey fungal spores or yeasts to their offspring. Although most stag beetles (Coleoptera: Lucanidae) are known to have female-specific mycangia and associated yeast symbionts, the evolutionary origin of the mycangium in this group remains unresolved. Here, we report the presence of a mycangium and associated yeast symbionts in the European horned stag beetle Sinodendron cylindricum (L.), which belongs to an ancestral clade of the Lucanidae. The mycangium of S. cylindricum is shown to be female-specific and have the same developmental origin as that of other stag beetles. A total of five yeast strains were isolated from adult mycangia and larval gut of S. cylindricum. Of these, we suggest that SICYAM1 is an undescribed yeast with taxonomic novelty, and have identified SICYLG3 as the xylose-fermenting yeast Scheffersomyces insectosa using nuclear ribosomal RNA and ITS sequences. The remaining three yeast strains, SICYAM2, SICYLG1, and SICYLG2, were assigned to the genus Sugiyamaella. Yeast density in the adult mycangium was lower than that of the more evolutionarily advanced stag beetles, the European Lucanus cervus (L.) and Dorcus parallelipipedus (L.), which were also examined in this study. No living yeasts were isolated from the adult guts. However, a third instar larva of S. cylindricum harbored 104–106 living yeasts in each gut region, which suggests that gut yeasts play an important role in these wood-feeding larvae. PMID:27432353

  17. Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

    Science.gov (United States)

    Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

    2012-04-01

    Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap. PMID:22606813

  18. Displacement of Tetropium cinnamopterum (Coleoptera: Cerambycidae) by Its Invasive Congener Tetropium fuscum.

    Science.gov (United States)

    Dearborn, Kenneth W; Heard, Stephen B; Sweeney, Jon; Pureswaran, Deepa S

    2016-08-01

    We examined the native community of insects interacting with an invasive species, Tetropium fuscum (F.) (Coleoptera: Cerambycidae), in its new range to explore reasons for the invader's relatively slow spread. Tetropium fuscum is a European spruce borer established in Nova Scotia since at least 1990, but it has spread only about 125 km from its site of introduction. We compared the densities of Tetropium spp., their known parasitoids, and the community of wood-boring insects at sites located within the invasion zone in Nova Scotia versus well outside this zone, in New Brunswick, Canada. Using red spruce trees stressed by girdling or felling, we tested whether: 1) T. fuscum had altered the native wood-boring community; 2) T. fuscum displaced a native congener, Tetropium cinnamopterum (Kirby); and 3) parasitism rates of Tetropium spp. differed between the invaded and noninvaded zones. Both Tetropium spp. and their parasitoid wasps emerged exclusively from felled trees as opposed to girdled trees. We found no difference in community diversity inside versus outside the invasion zone. The combined densities of both Tetropium spp. and their overall parasitism rates also did not differ between zones, but T. cinnamopterum density was significantly greater outside the invasion zone, suggesting T. fuscum may displace the native congener where they are sympatric. Our results suggest that the native and invasive Tetropium spp. act as a single functional species in the invasion zone. We speculate that natural control agents (predators, parasitoids, and competitors) might be limiting the rate of spread of T. fuscum. PMID:27247305

  19. Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil

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    Renata C. Campos

    2013-03-01

    Full Text Available Dung beetle assemblages (Coleoptera, Scarabaeinae in Atlantic forest fragments in southern Brazil. The beetles of the subfamily Scarabaeinae are important organisms that participate in the cycle of decomposition, especially in tropical ecosystems. Most species feed on feces (dung or carcasses (carrion and are associated with animals that produce their food resources. Dung beetles are divided into three functional groups: rollers, tunnelers and dwellers. This present work aims to study the diversity of dung beetle communities inhabiting fragments of the Atlantic Forest, with the purpose of describing the ecology of the species in southern Brazil. This study was conducted in the region of Campos Novos, in Santa Catarina, where twenty sites of Atlantic forest fragments were sampled. Samplings of dung beetles were conducted using 200 pitfall traps, of which 100 were baited with human feces and another 100 with carrion. Size and environmental complexity were also measured for each forest fragment. A total of 1,502 dung beetles, belonging to six tribes, 12 genera and 33 species, were collected. Results of the Levin's index of niche breadth indicated that 11 species were categorized as being coprophagous, ten as generalists, and two as necrophagous. Most species are tunnelers (19, nine of rollers and four of dwellers. The great diversity of Scarabaeinae in the region of Campos Novos, including several rare species, adds important data to the Scarabaeinae fauna in the central-western region of Santa Catarina. It may also help choosing priority areas for conservation in the region, where human impact, with large areas of monoculture, increasingly threatens the fragments of Mixed Ombrophilous Forest.

  20. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

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    G Mowlavi

    2008-12-01

    Full Text Available Background: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran.Methods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key Results: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorariusThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. Conclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  1. Scarab Beetle (Coleoptera: Scarabaeidae Fauna in Ardabil Province, North West Iran

    Directory of Open Access Journals (Sweden)

    G Mowlavi

    2008-12-01

    Full Text Available "nBackground: Dung beetles of Coleoptera associated to undisturbed cattle droppings in pastures present great diver¬sity and abundance. Dung beetles also play an important role for transmission of some helminthes to human and cat¬tle. This study was made to survey the biodiversity and abundance of these beetles in Ardebil Province, western Iran."nMethods: According to the field study all beetles attracted to fresh cow dung in five areas of Ardebil Province in¬cluding Namin, Ardabil, Meshkinshahr, Neer and Sarein were collected and identified. They were collected during summer 2007 from June to September, with general peaks appearing to be correlated with temperature mainly at 11 a.m to 15 p.m. The samples were identified using appropriate systematic key "nResults: A total of 231 specimens belonging to 9 beetle genera and at least 15 species were identified as Euoniticel¬lus fulvus, Sisyphus schaffaer, Euonthophagus taurus, Copris lunaris, Chironitis pamphilus, Gymnopleurus coriarus, Euonthophagus amyntas, Caccobius schreberi, Onthophagus speculifer, Onthophagus furcatus, Aphodius, lugens, Apho¬dius fimetarius, A. scrutator, Geotrupes spiniger and G. stercorarius"nThe most abundant and diverse subfamilies were Coprinae, Geotrupinae, and Aphodiinae. "nConclusion: We found 15 species of dung beetles occurred in the region. The prevalence of each species is varied depending on location. Some of them play an important role for helminths transmission of veterinary and public health importance. The finding will provide a clue for pasture management as well as public health monitoring and surveillance of the disease transmitted by dung beetles. 

  2. A comparison of trap type and height for capturing cerambycid beetles (Coleoptera).

    Science.gov (United States)

    Graham, Elizabeth E; Poland, Therese M; McCullough, Deborah G; Millar, Jocelyn G

    2012-06-01

    Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid species by testing some known cerambycid attractants and a pheromone in different trap designs placed across a range of habitats. We compared numbers and species richness of cerambycid beetles captured with cross-vane panel traps and 12-unit Lindgren multiple-funnel traps, placed either at ground level (1.5 m high) or canopy level (approximately 3-10 m high), at eight sites classified as either residential, industrial, deciduous forest, or conifer forest. We captured 3,723 beetles representing 72 cerambycid species from 10 June to 15 July 2010. Species richness was highest for the subfamilies Cerambycinae and Lamiinae, which accounted for 33 and 46% of all species captured, respectively. Overall, the cross-vane panel traps captured approximately 1.5 times more beetles than funnel traps. Twenty-one species were captured exclusively in traps at one height, either in the canopy or at ground level. More species were captured in hardwood sites (59 species) where a greater diversity of host material was available than in conifer (34 species), residential (41 species), or industrial (49) sites. Low numbers of beetles (n < 5) were recorded for 28 of the beetle species. The number of species captured per week ranged from 49 species on 21 June to 37 species on 12 July. Cross-vane panel traps installed across a vertical gradient should maximize the number of cerambycid species captured. PMID:22812119

  3. Tropical Plant Extracts as Sources of Grain-Protectant Compounds Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae).

    Science.gov (United States)

    Ribeiro, L P; Vendramim, J D; Andrade, M S; Bicalho, K U; Silva, M F G F; Vieira, P C; Fernandes, J B

    2014-10-01

    The toxicity of organic plant extracts to Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) was assessed for three tropical plant species: branches, leaves, and seeds of Annona montana (Annonaceae), branches of Aristolochia paulistana (Aristolochiaceae), and leaves and branches of Casearia sylvestris (Salicaceae). The screening assay resulted that the extracts of A. montana seeds obtained with hexane (LC50 = 534.75 mg kg(-1) and LT50 = 6.10 days) and with dichloromethane (LC50 = 424.67 mg kg(-1) and LT50 = 5.03 days) were the most promising treatments, followed by the extract prepared from A. montana leaves with hexane (LC50 = 837.70 mg kg(-1) and LT50 = 4.90 days). Moreover, extracts (at 1,500 mg kg(-1)) prepared from C. sylvestris branches with dichloromethane and A. paulistana with hexane caused significant mortality (37% and 41.5%, respectively) beyond sublethal effects on S. zeamais. Therefore, based on the biological assays, extraction yield, and evaluation of the chromatographic profile of the crude extracts by TLC, the hexane extract of A. montana seeds was selected and fractioned using liquid-liquid partitioning. The hydroalcoholic fraction caused mortality of 55.5%, significantly superior to dichloromethane fraction, which caused 35.5% of mortality. Chemical analyses ((1)H NMR, HPLC, and TLC) were performed, and the results showed the presence of alkaloids and acetogenins in both active fractions, which have been associated with bioactivity. Therefore, extracts prepared from A. montana seeds (especially) is an interesting source of new compounds with promising grain-protectant properties. PMID:27193958

  4. Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments.

    Science.gov (United States)

    Meikle, William G; Holst, Niels; Cook, Steven C; Patt, Joseph M

    2015-06-01

    Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32°C, but did at 28°C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28°C produced more larval mass than at 32°C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. PMID:26470208

  5. Comparative resistance of Russian and Italian honey bees (Hymenoptera: Apidae) to small hive beetles (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Frake, Amanda M; De Guzman, Lilia I; Rinderer, Thomas E

    2009-02-01

    To compare resistance to small hive beetles (Coleoptera: Nitidulidae) between Russian and commercial Italian honey bees (Hymenoptera: Apidae), the numbers of invading beetles, their population levels through time and small hive beetle reproduction inside the colonies were monitored. We found that the genotype of queens introduced into nucleus colonies had no immediate effect on small hive beetle invasion. However, the influence of honey bee stock on small hive beetle invasion was pronounced once test bees populated the hives. In colonies deliberately freed from small hive beetle during each observation period, the average number of invading beetles was higher in the Italian colonies (29 +/- 5 beetles) than in the Russian honey bee colonies (16 +/- 3 beetles). A similar trend was observed in colonies that were allowed to be freely colonized by beetles throughout the experimental period (Italian, 11.46 +/- 1.35; Russian, 5.21 +/- 0.66 beetles). A linear regression analysis showed no relationships between the number of beetles in the colonies and adult bee population (r2 = 0.1034, P = 0.297), brood produced (r2 = 0.1488, P = 0.132), or amount of pollen (P = 0.1036, P = 0.295). There were more Italian colonies that supported small hive beetle reproduction than Russian colonies. Regardless of stock, the use of entrance reducers had a significant effect on the average number of small hive beetle (with reducer, 16 +/- 3; without reducer, 27 +/- 5 beetles). However, there was no effect on bee population (with reducer, 13.20 +/- 0.71; without reducer, 14.60 +/- 0.70 frames) or brood production (with reducer, 6.12 +/- 0.30; without reducer, 6.44 +/- 0.34 frames). Overall, Russian honey bees were more resistant to small hive beetle than Italian honey bees as indicated by fewer invading beetles, lower small hive beetle population through time, and lesser reproduction. PMID:19253612

  6. Efficacy of natural products against Callosobruchus chinensis (Coleoptera: Bruchidae) in Nepal.

    Science.gov (United States)

    Regmi, Homan; Kafle, Lekhnath; Gc, Yubak Dhoj; Shih, Cheng Jen

    2012-06-01

    This study was conducted to determine the efficacy of local natural products against the beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae), in stored chickpea Cicer arietinum L. (Fabaceae) in Chitwan, Nepal. Five natural products and one synthetic pesticide (Malathion) and two storage regimes (aluminum sheet bin vs. jute bags with plastic lining) were tested for their effect on stored pulse with respect to damage by C. chinensis. The five natural products included Xanthoylum armatum DC (Rutaceae) fruit powder, Acorus calamus L. (Araceae) rhizome powder, Cinnamom camphora L. (Lauraceae) balls, oil of Sesamum indicum L. (Pedaliaceae), and leaf powder of Azadirachta indica A. Juss. (Meliaceae). Treatments of stored pulses with natural products or with Malathion all caused significantly higher mortality of C. chinensis at 15 d after treatment (DAT) than stored pulses receiving no treatments. The balls of C. camphora, rhizome powder of A. calamus and sesame oil outperformed all other treatments, including the Malathion at 45 and 75 DAT and resulted in significantly reduced damage or deterioration of stored pulses compared with other treatments. Storage regimes performed similarly, although the jute bags did protect seed integrity for some treatments. Our results indicate that incorporating these natural products into stored pulses can protect the seeds from C. chinensis for up to two generations, something that Malathion cannot do. These products are readily available to most farmers in the region and their use will lead to 1) reduction of losses to significant stored product pests, and 2) a reduction of contamination of foodstuffs and the environment by synthetic pesticides like Malathion. PMID:22812152

  7. Mortality of a wireworm, Agriotes obscurus (Coleoptera: Elateridae), after topical application of various insecticides.

    Science.gov (United States)

    Van Herk, W G; Vernon, R S; Tolman, J H; Saavedra, H Ortiz

    2008-04-01

    Ten insecticides representing seven chemical groups were applied at various concentrations topically by using a Potter Spray Tower to evaluate their relative toxicities on the European wireworm Agriotes obscurus L. (Coleoptera: Elateridae). Wireworms were stored at 15 degrees C after exposure to organophosphate (OP) (chlorpyrifos, diazinon), pyrethroid (tefluthrin), thianicotinoid (thiamethoxam, clothianidin), chloronicotinoid (imidacloprid, acetamiprid), phenyl pyrazole (fipronil), organochlorine (lindane), and spinosyn (spinosad) insecticides, and their postapplication health was evaluated weekly for up to 301 d. LC50, LC90, LT50, and LT90 values were calculated for each chemical except acetamiprid, and compared with those of lindane, clothianidin, and chlorpyrifos. Wireworms exposed to OPs died or recovered more quickly (LT50 < 20 d, LT90 < 50 d), than those exposed to all other insecticides tested except tefluthrin (LT50 = 25.5 d, LT90 = 66.5 d). Wireworms exposed to sublethal concentrations of all neonicotinoids quickly became moribund after application but made a full recovery. Wireworms exposed to fipronil at concentrations near the LC90 value showed no intoxication symptoms for up to 35 d, and they did not recover after symptoms developed. For each chemical, increasing the concentration increased the time required for wireworms to recover but decreased the time required to kill wireworms. Fipronil was highly toxic to wireworms (LC50 = 0.0001%), but acetamiprid (LC50 = 1.82%), imidacloprid (LC50 = 0.83%), tefluthrin (LC50 = 0.23%), diazinon (LC50 = 0.54%), and spinosad (LC50 = 0.51%) were not. The toxicity of both clothianidin (LC50 = 0.07%) and thiamethoxam (LC50 = 0.17%) were similar to those oflindane (LC50 = 0.06%) and chlorpyrifos (LC50 = 0.10%). PMID:18459401

  8. Nutritional Physiology of the Khapra Beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae) Fed on Various Barley Cultivars.

    Science.gov (United States)

    Seifi, S; Naseri, B; Razmjou, J

    2016-02-01

    The Khapra beetle, Trogoderma granarium Everts (Coleoptera: Dermestidae), is known as one of the mostserious pests of grains in many parts of the world. In this study, the effect of nine barley cultivars (‘Bahman’,‘CB-84-10’, ‘Fajr 30’, ‘Makuyi’, ‘Nosrat’, ‘Yousof’, ‘13A1’, ‘18A1’, and ‘19 A1’) and a wheat cultivar (‘MV17’, as a control) was determined on the nutritional indices and digestive enzymatic activity of T. granarium at 33 6 1C,relative humidity of 6565%, and a photoperiod of 14:10 (L:D) h. The highest and lowest values of larval weight gain of sixth instar were detected on wheat (0.757±0.068 mg) and cultivar Bahman (0.342±0.071 mg). Also, T. granarium larvae fed on cultivar Bahman had the lowest value of efficiency of conversion of ingested food(10.90±2.09%) as compared with wheat and other barley cultivars. Also, the highest midgut amylolytic and proteolytic activities of sixth instar were on cultivar Bahman (0.364±0.024 mU/mg and 80.54±1.73 U/mg, respectively)and the lowest activities were on cultivar Nosrat (0.043±0.004 mU/mg and 7.15±0.01 U/mg, respectively).It is concluded that barley cultivar Bahman was the most unsuitable host for feeding of T. granarium. PMID:26612893

  9. Distribución y patrones de diversidad de los Afódidos en la Comunidad de Madrid (Coleoptera, Scarabaeoidea, Aphodiidae, Aphodiinae y Psammodiinae

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    Hortal, J.

    2006-05-01

    Full Text Available In this work we present an updated checklist of the Aphodiidae (Coleoptera, Scarabaeoidea from Comunidad de Madrid (Spain. In addition, the observed and potential distributions of the 70 species found in Madrid are mapped. The potential spatial distributions of species richness, rarity and endemism in this region are also mapped. Finally, we discuss briefly the origin of the observed patterns.

    En este trabajo se presenta un inventario actualizado de los Aphodiidae (Coleoptera, Scarabaeoidea de la Comunidad de Madrid. Además, se presentan mapas de la distribución observada y potencial de las 70 especies encontradas en Madrid, así como de la distribución espacial de la riqueza, rareza, y endemicidad potencial en la región. Finalmente, se discute brevemente el origen de los patrones observados.

  10. Mass Trapping and Classical Biological Control of Rhynchophorus palmarum L. 1794 (Coleoptera: Curculionidae. A hypothesis based in morphological evidences.

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    Flávio Miguens

    2011-07-01

    Resumo. Coleópteros têm sido descritos como pragas e vetores de nematódeos causadores de Anel Vermelho em diversas palmeiras da família Arecaceae. Rhynchophorus palmarum L (Coleoptera: Curculionidae é uma praga que afeta a cocoicultura e outras palmeiras; e, vetor de Bursaphelencus cocophilus (Cobb Baujard (Nematoda, agente etiológico de Anel Vermelho e de outros nematódeos. Atualmente, recomenda-se o emprego de inimigos naturais e parasitas no manejo integrado de pragas; dentre elas, Rhynchophorinae. Armadilhas de coleta massal são recomendadas no manejo integrado de pragas. Nosso estudo relata, na cocoicultura, a eficiência de armadilhas artesanais de baixo custo e a utilização cariomônios (toletes de cana-de-açúcar e cariômonios mais feromônios (toletes de cana-de-açúcar e machos adultos de R. palmarum como atrativos nas armadilhas. Ácaros ectoparasitas foram identificados nestes coleópteros, por microscopia, que podem ser propostos como parte do manejo integrado desta praga. Armadilhas de coleta massal com cariômonios foram eficientes na captura de R. palmarum e outros Curculionidae. No entanto, armadilhas de coleta massal com cariômonios e feromônios aumentaram a atratividade, em relação às primeiras, para este Coleoptera e Cosmopolites sordidus (Germar (Coleoptera: Curculionidae. Eventualmente, exemplares de R. palmarum apresentavam infecção fúngica. Ácaros ectoparasitas infestavam mais de 50% dos exemplares de R. palmarum. A microanatomia destes ácaros e sua interação com R. palmarum foi preliminarmente descrita. Todos os estágios do ciclo de vida destes ácaros foram identificados no compartimento dos élitros. As evidências morfológicas suportam a hipótese de que estes ácaros podem ser empregados no controle biológico de R. palmarum em um programa de manejo integrado.

  11. Estrutura e organização de assembleias de Scarabaeinae (Coleoptera, Scarabaeidae em diferentes fitofisionomias no sul do Brasil

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    Jonas Darci Noronha de Lima

    2015-12-01

    Full Text Available RESUMO Padrões de riqueza de espécies, abundância, diversidade, equitabilidade e dominância, além da organização em guildas tróficas e comportamentais da fauna de Scarabaeinae foram analisados em três diferentes fitofisionomias (floresta, campo natural e lavoura na região noroeste do estado do Rio Grande do Sul, Brasil, entre setembro e novembro de 2012, através da utilização de armadilhas de queda iscadas com distintos recursos (fezes, carne em decomposição e banana fermentada. Foram capturados 9.325 espécimes, distribuídos em nove gêneros e 32 espécies. As espécies mais abundantes foram Onthophagus aff.tristis Harold, 1873, Canthidium aff.trinodosum (Boheman, 1858, Canthon aff.fallax Harold, 1868, Canthon lividusBlanchard, 1845 e Deltochilum morbillosum Burmeister, 1848 que representaram 65,54% do total de indivíduos amostrados. A floresta apresentou os valores mais elevados de número de indivíduos e de espécies. A menor riqueza foi observada no campo, enquanto a lavoura apresentou a menor abundância de indivíduos. Scarabaeinae apresentou diferenças quali- e quantitativas em suas assembleias pelas fitofisionomias amostradas. A floresta abrigou a maior riqueza observada de espécies e uma fração destas é exclusiva deste ambiente, e dificilmente ocorre em outros tipos de ecossistemas. A floresta apresentou uma proporção maior de espécies generalistas ou copro-necrófagas em sua composição. Outra parte desta fauna, representada por espécies coprófagas, está adaptada ao ambiente aberto indicando uma modificação na guilda alimentar causada pela substituição da floresta por pastagem. Dessa forma, em um contexto mais amplo a paisagem pode desempenhar um papel importante na diversidade de Scarabaeinae.

  12. Notes on Diochus Erichson, Lissohypnus Casey, and Oxybleptes Smetana (Coleoptera: Staphylinidae) in Florida, including a description of a new species of Lissohypnus

    OpenAIRE

    Frank, J. Howard; Kelly, Sandor L.; Almquist, David T.

    2014-01-01

    The known range of Oxybleptes meridionalis Smetana (Coleoptera: Staphylinidae) is expanded in Florida, USA, from Indian River and Manatee counties to now include Brevard, Highlands, Orange, Seminole and Volusia. Oxybleptes davisi (Notman) is confi rmed to exist in Florida, with records from Leon, Liberty and Wakulla counties in the Panhandle, and Orange County in central Florida. Lissohypnus texanus Casey is newly reported from Florida. A new species, Lissohypnus fullertoni, is described from...

  13. Description of a new species of Plesioclytus Giesbert (Coleoptera: Cerambycidae: Cerambycinae) from Georgia, and transfer of the genus to Plesioclytini Wappes and Skelley, new tribe

    OpenAIRE

    Wappes, James E.; Skelley, Paul E.

    2016-01-01

    Plesioclytus morrisi Wappes and Skelley new species (Coleoptera: Cerambycidae) from the Ohoopee dune system in central Georgia is described with comments on the biology of the new species. The taxonomic placement of Plesioclytus Giesbert in the tribe Clytini is questioned as key characters are found to differ from the current characters used to define the tribe in the New World, resulting in its transfer to the newly erected Plesioclytini Wappes and Skelley new tribe, defined herein. Habitat ...

  14. A new species of Drapetes Megerle (Coleoptera: Elateridae), with taxonomic summaries and a key to the species of northern North America

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    Johnson, Paul J.

    2016-01-01

    Seven species of Drapetes Megerle (Coleoptera: Elateridae) are recognized from northern North America. Drapetes chiricahua new species is described from southeastern Arizona and north-central New Mexico. Drapetes parallelus Van Dyke is a senior homonym of Drapetes paralellus Cobos by variant spelling, and a new synonym of Drapetes cylindricus Fall. Drapetes paralellus Cobos is replaced with Drapetes cobosi new name. Drapetes clarki Bonvouloir is not a North American species and is given a red...

  15. A simultaneous journal / wiki publication and dissemination of a new species description: Neobidessodes darwiniensis sp. n. from northern Australia (Coleoptera, Dytiscidae, Bidessini)

    OpenAIRE

    Lars Hendrich; Michael Balke

    2011-01-01

    Abstract Here, we describe a new Australian species in journal format and simultaneously open the description in a wiki format on the www.species-id.net. The wiki format will always link to the fixed original journal description of the taxon, however it permits future edits and additions to species' taxonomy and biology. The diving beetle Neobidessodes darwiniensis sp. n. (Coleoptera: Dytiscidae, Bidessini) is described based on a single female, collected in a rest pool of the Harriet Creek i...

  16. A scientific note on a new pest for European honeybees: first report of small hive beetle Aethina tumida, (Coleoptera: Nitidulidae) in Italy

    OpenAIRE

    Palmeri, Vincenzo; Scirtò, Giuseppe; Malacrinò, Antonino; Laudani, Francesca; Campolo, Orlando

    2015-01-01

    International audience The small hive beetle (SHB), Aethina tumida Murray(Coleoptera Nitidulidae), is an important pest of honeybeesin many parts of the world. The beetle is native tosub-Saharan Africa and was unintentionally introducedinto USA (1996), Australia (2001) and in Canada(2002) (Ellis and Munn, 2005; Neumann and Ellis2008). In Europe the SHB was discovered in 2005 ina consignment of queens imported from Texas (USA)into Portugal and destroyed by the Portuguese NationalVeterinaria...

  17. Plant compounds insecticide activity against Coleoptera pests of stored products Compostos de plantas com atividade inseticida a coleópteros-praga de produtos armazenados

    OpenAIRE

    Márcio Dionizio Moreira; Marcelo Coutinho Picanço; Luiz Cláudio de Almeida Barbosa; Raul Narciso Carvalho Guedes; Mateus Ribeiro de Campos; Gerson Adriano Silva; Júlio Cláudio Martins

    2007-01-01

    The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed...

  18. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy

    OpenAIRE

    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  19. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.Bioatividade do mogno da Indonésia, Toona sureni (Blume (Meliaceae, contra o besouro-das-farinhas, Tribolium castaneum (Coleoptera, Tenebrionidae. A atividade inseticida de Toona sureni (Blume Merr. foi avaliada considerando repelência, mortalidade e a produção de progênie de adultos F1 de Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Extrato seco de sementes

  20. Hexamermis paranaense new species (Nematoda, Mermithidae: a parasite of Diloboderus abderus (Coleoptera, Scarabaeidae in Argentina Hexamermis paranaense sp. nov. (Nematoda, Mermithidae: parasito de Diloboderus abderus (Coleoptera, Scarabaeidae en Argentina

    Directory of Open Access Journals (Sweden)

    María Fernanda Achinelly

    2008-12-01

    Full Text Available A new species, Hexamermis paranaense n. sp. (Nematoda, Mermithidae, a parasite of larvae of Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae is described and illustrated. This new species is characterized by amphids small, amphidial opening pocket-shaped, the anterior portion of the vagina muscularized and slightly protruding with a descending branch forming a loop before joining the uterus, and three rows of genital papillae: the ventrolateral divided in two rows with eight papillae in the outer row and with six papillae in the inner one; the ventral row with four pairs and one single preanal papillae, and with two pairs, a triplete, one pair, a single and one pair postanal papillae.Una nueva especie, Hexamermis paranaense sp. nov. (Nematoda, Mermithidae, parásito de larvas del gusano blanco, Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae, se describe e ilustra. Esta nueva especie se caracteriza por poseer anfidos pequeños, con la apertura en forma de bolsillo, parte anterior de la vagina muscular, con una rama que desciende y forma un lazo antes de la conexión al útero, tres hileras de papilas genitales: la hilera de posición ventro-lateral dividida en dos hileras con ocho papilas en la hilera externa y con seis papilas en la interna; la hilera ventral con una papila, seguida de cuatro pares, todas en posición preanal y dos pares, interrumpidos por tres papilas, seguida de un par, una simple, y un par, todas de posición postanal.

  1. Morphological and isozymic banding pattern study of white grubs (Coleoptera: Melolonthidae as pest of bark crop in mounth Merapi’s slope.

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    SUGIYARTO

    2008-07-01

    Full Text Available White grub (Coleoptera: Melolonthidae is a group of soil pest at any agrosystem., especially at Salak pondoh (Salacca zalacca (Gaert. Voss. crop. The characteristics of this specimen were very crucial to be studied in order to find the exact biocontrol. The aim of this research was to know the characteristics of white grubs (Melolonthidae: Coleoptera based on morphological and isozyme banding patterns. This research was conducted on August - November 2007 at Sleman and Magelang districts for the morphological purposes, while for the isozyme data were conducted at Sub Laboratory Biology, Central Laboratory of Sebelas Maret University Surakarta. Sample was taken by using stratified random sampling method, on five stations. Polyacrylamide gel electrophoresis (PAGE using the vertical type was taken to isozyme analysis. The enzyme used in this research were peroxidase and esterase to detect the isozyme banding patterns. The results showed that there was no morphological variation of white grubs (Melolonthidae: Coleoptera at salak pondoh agroecosystem in Mounth Merapi’s slope. Based on this character, there was one species of white grub found, i.e. Holotrichia javana. There was a genetic variation based on the variation of isozyme banding patterns.

  2. Description of the female of Haematonotus jenisi Krajcik, 2006 and placement of the species within the genus Atrichiana Distant, 1911 (Coleoptera, Scarabaeidae, Cetoniinae)

    Science.gov (United States)

    Perissinotto, Renzo

    2016-01-01

    Abstract The male of Haematonotus jenisi Krajcik, 2006 was described on the basis of a sole holotype specimen. A number of new specimens have recently been collected from the False Bay area of the iSimangaliso Wetland Park, in northeastern KwaZulu-Natal, including a number of females, thereby allowing the description of this sex for the first time. The new series also reveals that most morphological characters are actually typical for the genus Atrichiana Distant, 1911, rather than Haematonotus Kraatz, 1880. For this reason, it is here proposed that this species be placed within Atrichiana, thus becoming the second species of a genus that was previously regarded as monospecific, represented by the southern African endemic Atrichiana placida (Boheman, 1857). Like Atrichiana placida, Atrichiana jenisi exhibits a remarkable polymorphism, but also sexual dimorphism, particularly expressed at the level of the antennal clubs, which in the male are twice as long as in the female. Unfortunately, all specimens of the recent series were collected dead or drowning on the lake shores and therefore no further information on their life cycle and biology in general could be obtained. It seems that the species may represent a micro-endemism restricted to the Western Shores of Lake St Lucia, with habitat associated to Sand Forest and possibly also Northern Coastal Forest vegetation types. PMID:27006615

  3. A PCR-Based Diagnostic System for Differentiating Two Weevil Species (Coleoptera: Curculionidae) of Economic Importance to the Chilean Citrus Industry.

    Science.gov (United States)

    Aguirre, C; Olivares, N; Luppichini, P; Hinrichsen, P

    2015-02-01

    A PCR-based method was developed to identify Naupactus cervinus (Boheman) and Naupactus xanthographus (Germar), two curculionids affecting the citrus industry in Chile. The quarantine status of these two species depends on the country to which fruits are exported. This identification method was developed because it is not possible to discriminate between these two species at the egg stage. The method is based on the species-specific amplification of sequences of internal transcribed spacers, for which we cloned and sequenced these genome fragments from each species. We designed an identification system based on two duplex-PCR reactions. Each one contains the species-specific primer set and a second generic primer set that amplify a short 18S region common to coleopterans, to avoid false negatives. The marker system is able to differentiate each Naupactus species at any life stage, and with a diagnostic sensitivity to 0.045 ng of genomic DNA. This PCR kit was validated by samples collected from different citrus production areas throughout Chile and showed 100% accuracy in differentiating the two Naupactus species. PMID:26470110

  4. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    Science.gov (United States)

    Hönnicke, M. G.; Cusatis, C.; Rigon, L.; Menk, R.-H.; Arfelli, F.; Foerster, L. A.; Rosado-Neto, G. H.

    2010-08-01

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures ( Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  5. Predation potential of Chilocorus cacti (Coleoptera: Coccinellidae) to the prickly pear cacti pest Dactylopius opuntiae (Hemiptera: Dactylopiidae).

    Science.gov (United States)

    Flores, A; Olvera, H; Rodríguez, S; Barranco, J

    2013-08-01

    Functional response of the predator Chilocorus cacti (Linnaeus) (Coleoptera: Coccinellidae) on five densities of Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae) female adults was assessed under laboratory conditions. The searching efficiency of C. cacti significantly decreased as prey density increased. The logistic regression for the predator had a negative and significant linear parameter indicating a type II functional response. Non-linear regression for Holling predator equation estimated a handling time of 1.79 ± 0.129 h and attack rate coefficient of 0.1003 ± 0.030. Most of this handling time was because the predator spent a lot of time removing the waxy coating that protects adult females of D. opuntiae. Chilocorus cacti consumes females of D. opuntiae in their reproductive stage; therefore, it could be an effective natural enemy to suppress or regulate low density populations of D. opuntiae, preventing them to reach high densities. PMID:23949861

  6. Characterization of the proteases in the midgut of the xylophagous larvae of Oemona hirta (Coleoptera:Cerambycidae)

    Institute of Scientific and Technical Information of China (English)

    Brian David Shaw; John Tane Christeller

    2009-01-01

    The protein digestive capability oftbe larvae of the longhorn beetle (Oemona hirta,Coleoptera:Cerambycidae,Fabricius,1775) was investigated.This species feeds only on wood where there is a high proportion of vascular tissue.The pH of the midgut,the major digestive organ,was alkaline and protein hydrolysis was maximal at alkaline pH.Use of specific synthetic peptide substrates showed that the major protease activities were the endopeptidases,trypsin and chymotrypsin-like activity,and the exopeptidase,leucine aminopeptidase and the pH curves corresponded to that with protein substrate.Studies using a range ofsefine protease inhibitors as well as specific inhibitors ofmetalloproteases,cysteine proteases and aspartate proteases confirmed a serine protease-based digestive system similar to earlier reports of sapwood-feeding Cerambycids.Control of these insect pests using protease inhibitors is discussed.

  7. Toxicities of azadirachtin and polychlorinated petroleum Hydrocarbon against resist and susceptible strains of tribolium castaneum (coleoptera: tenebrionidae) adults

    International Nuclear Information System (INIS)

    The LC/sub 50/ values for malathion-resistant (PAK) and organo-50 phosphate-susceptible (FSS-II) strains of red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) adult beetles were determined through filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS-II strains, respectively. The results revealed that polychlorinated petroleum hydrocarbon was more toxic than the azadirachtin. Furthermore, both the insecticides were equally toxic to the adult beetles of T. castaneum as the difference was non-significant because of overlapping 95% FLs to LC./sub 50/. (author)

  8. External and internal structure of weevils (Insecta: Coleoptera) investigated with phase-contrast X-ray imaging

    International Nuclear Information System (INIS)

    Weevils (Coleoptera: Curculionidae) are identified by the external structure (dorsal, ventral and lateral features) and also by internal structure. The genitalia can be used to distinguish the sex and to identify the insects when the external structure appears identical. For this purpose, a destructive dissecting microscopy procedure is usually employed. In this paper, phase contrast X-ray imaging (radiography and tomography) is employed to investigate the internal structure (genitalia) of two entire species of weevils that presents very similar external structures (Sitophilus oryzae and Sitophilus zeamais). The detection of features, which looks like the genital structure, shows that such non-destructive technique could be used as an alternative method for identification of insects. This method is especially useful in examining the internal features of precious species from museum collections, as already described in the recent literature.

  9. The effects of temperature, diet, and other factors on development, survivorship, and oviposition of Aethina tumida (Coleoptera: Nitidulidae).

    Science.gov (United States)

    Meikle, William G; Patt, Joseph M

    2011-06-01

    Developmental rate and survivorship of small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), life stages were measured across different temperatures (21, 25, 28, 32 and 35 degrees C) and diets, which included natural and artificial pollen, honey, and bee pupae. Temperature affected hatch success, time to hatching, and larval growth. Eggs hatched in 61 h at 21 degrees C but in Beetles lived longer at 28 degrees C or lower but produced the most eggs per female, regardless of diet, at 32 degrees C. Beetle density influenced fecundity: beetles kept at three pairs per vial laid 6.7 times more eggs per female than those kept as single pairs. Overall, beetles fared best at 28-32 degrees C with mortality of all stages highest at 35 degrees C. PMID:21735891

  10. Genetic differentiation among populations of the beetle Bolitophagus reticulatus (Coleoptera: tenebrionidae) in a fragmented and a continuous landscape.

    Science.gov (United States)

    Knutsen, H; Rukke, B A; Jorde, P E; Ims, R A

    2000-06-01

    The effect of habitat fragmentation on genetic differentiation among local populations of the fungivorous beetle Bolitophagus reticulatus (Coleoptera: Tenebrionidae) was studied in two contrasting landscapes: one heavily fragmented with forest fragments of variable size surrounded by inhabitable agricultural fields, the other an old forest providing a continuous habitat. The genetic structure of the beetle within each of the two contrasting areas was investigated by means of protein electrophoresis, screening four polymorphic loci in 20 populations from each area. In both areas there were significant genetic differences among local populations, but on average differentiation in the fragmented area was three times greater than in the continuous one, strongly indicating a genetic isolation effect of habitat fragmentation. These genetic results are in accordance with previous studies on dispersal in this species. PMID:10886382

  11. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses. PMID:23786050

  12. Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae

    Directory of Open Access Journals (Sweden)

    Sahana Parvin

    2012-09-01

    Full Text Available Bioactivity of Indonesian mahogany, Toona sureni (Blume (Meliaceae, against the red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae. The insecticidal activity of Toona sureni (Blume Merr. was evaluated considering repellency, mortality and progeny production of F1 adults of Tribolium castaneum (Herbst, 1797 (Coleoptera, Tenebrionidae. Dried extract of seeds of T. sureni was dissolved in acetone to prepare solution of various concentrations (0.5, 1.0, 2.5 and 5.0%. To test for repellency, the insects were exposed to treated filter paper. Mortality of larvae, pupae and adults was evaluated by the treatment of spraying the insects with different concentrations of T. sureni extract. Residual effect of the extract was also evaluated considering the production of progeny of F1 adults. The highest repellency (93.30% of T. castaneum occurred at the highest concentration (5.0% suspension of T. sureni; while the lowest (0.0% repellency occurred at 0.5% suspension after 1 day of treatment. The highest mortality against adults (86.71%, larvae (88.32% and pupae (85% occurred at 5% suspension at 8 days after application. There was a negative correlation between the concentrations of T. sureni and the production of F1 adult's progeny of T. castaneum. The highest number of progeny (147 of T. castaneum occurred in the control at 7 days after treatment; and the lowest number of progeny (43 occurred at 5.0% concentration in 1 day after treatment. The results show that T. sureni is toxic to T. castaneum and has the potential to control all stages of this insect in stored wheat.

  13. Parasitylenchus bifurcatus n. sp. (Tylenchida: Allantonematidae parasitizing Harmonia axyridis (Coleoptera: Coccinellidae

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    Poinar George O

    2012-10-01

    Full Text Available Abstract Background The harlequin ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae is native to central and eastern Asia and was purposely introduced into Europe to control aphids. While it proved to be a good biological control agent, its rapid spread and buildup of large populations made it a nuisance, since it overwinters in homes, emits unpleasant odors, stains fabrics, occasionally bites humans and feeds on apples, pears and grapes. Aside from the above, the ravenous appetite of H. axyridis results in their consumption of harmless native insects, including even other ladybird beetles. A study of the natural enemies of H. axyridis in Denmark revealed the presence of nematodes. The present study describes this nematode parasite and discusses aspects of its development and ecology. Methods Adult harlequin ladybird beetles were collected from March to November from four localities in Copenhagen on different plant species. In addition, groups of last-instar larvae and pupae (n = 50 were examined for the presence of nematodes. Living and recently dead nematodes were removed from adult H. axyridis in 0.5% saline solution, the nematodes were then heat killed (at 75C, fixed in 5% formalin and transferred to glycerin on slides for further examination and measurements. Results A new species of Allantonematidae (Tylenchida, Parasitylenchus bifurcatus n. sp., is described from adults of the harlequin ladybird, Harmonia axyridis in Denmark. The new species is characterized by a straight stylet lacking basal thickenings, a bursa and a forked tail tip in the vermiform (infective females and juvenile males. The new species is compared with P. coccinellinae previously described from ladybird beetles in France. Parasitism resulted in depletion of the fat body and partial or complete atrophy of the reproductive organs of the beetles. Infections occurred throughout the year with rates of parasitism reaching up to 35%. The rate increased to 60

  14. Entomofauna Associada a Galhos de Acacia mangium Willd. Roletados por Oncideres saga (Dalman (Coleoptera: Cerambycidae

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    Gláucia Cordeiro

    2010-04-01

    Full Text Available O estudo da entomofauna associada aos galhos e fustes roletados por Oncideres saga (Dalman é importante para conhecermos seus possíveis inimigos naturais. Portanto, este trabalho teve como objetivo registrar os insetos associados aos galhos e fustes de Acacia mangium Willd. roletados por O. saga, em Coimbra-MG. Galhos e fustes roletados de A. mangium foram coletados de janeiro a abril de 2007. Este material foi vistoriado, armazenados em sacolas, feitas com tela plástica, e mantidos em sala com condições controladas (25,4±0,3°C e 66,7±1,4%. Constatou-se a presença de uma espécie, não determinada, de Scolytidae e a emergência de quatro espécies de Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus e Neoclytus pusillus (Laporte & Gory. Isto posto, fica evidenciada a necessidade de estudos com o objetivo de verificar qual o comportamento destes insetos em relação ao serrador O. saga.Insects associated with branches of Acacia mangium Willd. girdled by Oncideres saga (Dalman (Coleoptera: CerambycidaeAbstract. The study of the insects associated with branches and stems girdled by Oncideres saga (Dalman is important to know its possible natural enemies. Therefore, these work had the objective of register the insects associated with branches and stems girdled of Acacia mangium Willd. by this twig girdler beetle, in Coimbra, state of Minas Gerais, Brazil. Stems and branches of A. mangium were collected in January/2007 to April/2007. This material has been inspected, stored in plastic bags, and kept in a room with controlled conditions (25.4 ± 0.3°C and 66.7 ± 1.4%. It was noted the presence of a non-determined species of Scolytidae and the emergence of four species of Cerambycidae: Engyum quadrinotatum Thomsom; Eburodacrys sexmaculata (Olivier; Achryson surinamum (Linnaeus and Neoclytus pusillus (Laporte & Gory. It can be concluded that studies are needed with the objective of

  15. Karyotypes of some medium-sized Dytiscidae (Agabinae and Colymbetinae (Coleoptera

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    Robert Angus

    2013-06-01

    Full Text Available An account is given of the karyotypes of 29 species of medium sized Dytiscidae (Coleoptera. Of the 20 species of Agabus Leach, 1817, 18 have karyotypes comprising 21 pairs of autosomes and sex chromosomes which are either X0(♂ or XX (♀. These species are A. serricornis (Paykull, 1799, A. labiatus (Brahm, 1791, A. congener (Thunberg, 1794, A. lapponicus (Thomson, 1867, A. thomsoni (J. Sahlberg, 1871, A. confinis (Gyllenhal, 1808, A. sturmii (Gyllenhal, 1808, A. bipustulatus (Linnaeus, 1767, A. nevadensis Håkan Lindberg, 1939, A. wollastoni Sharp, 1882, A. melanarius Aubé, 1837, A. biguttatus (Olivier, 1795, A. binotatus Aubé, 1837, A. affinis (Paykull, 1798, A. unguicularis (Thomson, 1867, A. ramblae Millan & Ribera, 2001, A. conspersus (Marsham, 1802 and A. nebulosus (Forster, 1771. However two species, A. infuscatus Aubé, 1838 and A. adpressus Aubé, 1837, have developed a neo-XY system, with karyotypes comprising 21 pairs of autosomes and XY sex chromosomes (♂. No chromosomal differences have been detected between typical A. bipustulatus and A. bipustulatus var. solieri Aubé, 1837, nor have any been found between the three species of the A. bipustulatus complex (A. bipustulatus, A. nevadensis and A. wollastoni. The four species of Colymbetes Clairville, 1806, C. fuscus (Linnaeus, 1758, C. paykulli Erichson, 1837, C. piceus Klug, 1834 and C. striatus (Linnaeus, 1758 have karyotypes comprising 20 pairs of autosomes and sex chromosomes which are X0 (♂, XX (♀. Two of the species of Rhantus Dejean, 1833, R. exsoletus (Forster, 1771 and R. suturellus (Harris, 1828 have karyotypes comprising 20 pairs of autosomes and X0/XX sex chromosomes, but the other three species, R. grapii (Gyllenhal, 1808, R. frontalis (Marsham, 1802 and R. suturalis (Macleay, 1825 have 22 pairs of autosomes and X0/XX sex chromosomes. Agabus congener and Rhantus suturellus may have one B-chromosome. Nine of the species have previously published karyotype data but

  16. Susceptibility of Agrilus planipennis (Coleoptera: Buprestidae) to Beauveria bassiana and Metarhizium anisopliae.

    Science.gov (United States)

    Liu, Houping; Bauer, Leah S

    2006-08-01

    The susceptibility of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) to selected strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin was evaluated through bioassays with direct immersion or foliar exposure under laboratory conditions. Results showed that A. planipennis adults were susceptible to B. bassiana and M. anisoplae. Significant time-mortality response was found for each isolates. Isolate B. bassiana GHA killed A. planipennis adults at a faster rate compared with other isolates tested, with the lowest average time-to-death values. The LC50 values estimated under direct immersion method ranged from 1.7 x 10(5) to 1.9 x 10(7), 3.5 x 10(4) to 5.3 x 10(5), and 4.1 x 10(3) to 2.9 x 10(5) conidia/ml for B. basissiana and from 3.2 x 10(6) to 1.1 x 10(7), 4.5 x 10(3) to 4.5 x 10(5), and 1.4 x 10(2) to 1.2 x 10(5) conidia/ml for M. anisopliae at 4, 5, and 6 d after treatment, respectively. By days 5 and 6, B. bassiana GHA outperformed all other isolates tested except ARSEF 7234, followed by ARSEF 7152, 6393, and 7180. Significant concentration-mortality response was also observed for two B. bassiana GHA formulations, BotaniGard ES and Mycotrol O, and M. anisopliae F52 when insects were treated through foliar exposure. The LC50 values ranged from 114.5 to 309.6, 18.4 to 797.3, and 345.3 to 362.0 conidia/cm2 for BotaniGard, Mycotrol, and M. anisopliae F52, respectively. Based on the results of these bioassays, the efficacy of both B. bassiana GHA formulations and M. anisopliae F52 were similar against adult A. planipennis. The potential use of entomopathogenic fungi for management of A. planipennis in North America is discussed. PMID:16937660

  17. Oryctini (Coleoptera: Scarabaeidae: Dynastinae da coleção de invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brasil Oryctini (Coleoptera: Scarabaeidae: Dynastinae of the invertebrate collection of the National Institute of Research of Amazonia (INPA, Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Hector Jaime Gasca

    2009-09-01

    Full Text Available A Coleção de Invertebrados do Instituto Nacional de Pesquisas da Amazônia (INPA possui 554 espécimes de Oryctini (Coleoptera: Scarabaeidae: Dynastinae, pertencentes a 7 gêneros, 16 espécies e 2 subespécies. As distribuições geográficas das espécies são fornecidas, sendo que 97% do material examinado procedem de coletas feitas em diferentes locais da Amazônia brasileira.The Invertebrate Collection of the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas, Brazil, has 554 specimens of Oryctini (Coleoptera: Scarabaeidae: Dynastinae, belonging to seven genera, 16 species and two subspecies. Information about geographical distribution are provided, of which 97% of the material examined was collected from several places in the Brazilian Amazon.

  18. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region Ocorrência e biologia de Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitando diferentes espécies de Coccinellidae (Coleoptera) na região Neotropical

    OpenAIRE

    RB Silva; I Cruz; MLC Figueiredo; AG Pereira; AM Penteado-Dias

    2012-01-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milh...

  19. Fauna de Coleoptera no Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brasil: abundância e riqueza das famílias capturadas através de armadilhas de solo Coleoptera fauna in the Parque Estadual de Vila Velha, Ponta Grossa, Paraná, Brazil: abundance and family richness captured with pitfall traps

    OpenAIRE

    Renato C. Marinoni; Norma G. Ganho

    2003-01-01

    This paper is a part of the studies on the Coleoptera fauna from Vila Velha, Ponta Grossa, Parana gathered through malaise and pitfall traps in sites with different floristic conditions. The present study deal with the data of pitfall trap captures, installed close to the malaise traps. The data were obtained weekly (52 samples), from September 1999 to August 2000. This survey was carried out on five areas, three of them with different plant succession stages (initial, intermediate, and advan...

  20. Biological and Morphological Aspects of Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), a Pest of Apulian Pinewoods in Southern Italy Aspetos da Biologia e Morfologia da Anoxia (Mesanoxia) matutinalis ssp. matutinalis Castelnau, 1832 (Coleoptera, Melolonthidae), Praga nos Pinhais da Apúlia, Sul de Itália

    OpenAIRE

    Eustachio Tarasco; Oreste Triggiani

    2011-01-01

    Huge defoliations by Anoxia (Mesanoxia) matutinalis spp. matutinalis Castelnau, 1832 (Coleoptera, Scarabeidae) have been observed in recent years in the coastal pinewoods (Pinus halepensis, P. pinea) of the southern Apulia Region of Italy. Starting from adults collected in the field, insect breeding was conducted in the laboratory, following the life-cycle from ovideposition to larval development and emergence. Observations were performed in the laboratory and the field to study the different...

  1. Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná A study of the Coleoptera (Insecta fauna that inhabits Sus scrofa L. carcass in Curitiba, Paraná

    Directory of Open Access Journals (Sweden)

    Kleber Makoto Mise

    2007-09-01

    Full Text Available Este trabalho visou levantar a fauna de Coleoptera associada à carcaça de Sus scrofa L.,1758, espécie utilizada como modelo em Entomologia Forense. O acréscimo ou a substituição seqüencial das espécies de insetos pode ser utilizado para estimar o intervalo post mortem (IPM. O experimento foi realizado no Centro Politécnico (UFPR, de setembro de 2005 a setembro de 2006. A cada estação foi sacrificado um suíno de 15 kg no local, colocado em gaiola. A captura dos insetos foi realizada diariamente em bandeja posicionada abaixo da carcaça e em armadilha tipo Shannon modificada, e a cada 14 dias em cinco armadilhas do tipo pit-fall. Foram coletados 4.360 Coleoptera, pertencentes a 112 espécies de 26 famílias, 12 consideradas de importância forense. A coleta ativa realizada na bandeja foi responsável pela maior captura (2.023 espécimes, seguida pela armadilha Shannon modificada (2.016 espécimes e por último pelas do tipo pit-fall (324 espécimes. Staphylinidae foi mais coletada na bandeja e Shannon modificada, e Silphidae na armadilha pit-fall. Os principais hábitos encontrados foram predador/parasita (55%e onívoro (38,05%, com poucas espécies consideradas necrófagas (1,31%.This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI. The present study took place in Centro Politécnico (UFPR, between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of

  2. First Record Of Clytra Laeviuscula Ratzeburg As Potential Insect Pest Of Energy Willow (Salix Viminalis L. In Ukraine

    Directory of Open Access Journals (Sweden)

    Stefanovska Tatyana

    2015-09-01

    Full Text Available Cultivation of short rotation coppice energy willow (SRC EW, Salix viminalis L., has a great potential in Ukraine as a source of biomass for biofuel production. Commercial production of this species was recently initiated in the country. The growing of SRC EW in Western and Northern Europe for a long time showed that leaf beetles (Coleoptera: Chrysomelidae are key pests causing significant biomass reduction. However, data about the pest complex for energy willow growing in Ukraine is not available. Our three-year experiment in Poltava region, Ukraine showed that foliar damage caused by Clytra laeviuscula Ratzeburg (Coleoptera: Chrysomelidae occurred at energy willow plantations in the second year of production, which could have an effect on commercial production. Accordingly, information about seasonal activity, population dynamics, host range and the role of natural enemies in pest regulation are requested for developing pest control program.

  3. Os besouros (Insecta: Coleoptera na concepção dos moradores de Pedra Branca, Santa Terezinha, Estado da Bahia = The term “beetle” (Insecta: Coleoptera as perceived and used by dwellers of Pedra Branca, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Eraldo Medeiros Costa Neto

    2006-01-01

    Full Text Available Besouros (Insecta: Coleoptera são percebidos, conhecidos e utilizados por moradores do povoado de Pedra Branca, localizado no Estado da Bahia, Brasil. O trabalho de campo foi realizado entre abril e dezembro de 2004. Os dados foram obtidos com 49 indivíduos (20 do sexo masculino e 29 do sexo feminino por meio de entrevistas semiestruturadas e gravadas, seguindo-se uma abordagem emicista. Os resultados mostram que o genérico “besouro” é usado como uma categoria etnotaxonômica que reúne organismos sistematicamente não relacionados, além dos coleópteros da classificação lineana. Foramcitados 43 nomes locais de besouros. Os informantes atribuíram características qualitativas aos “besouros”, muitas das quais são antropomórficas. Algumas etnoespécies de “besouros” causam danos aos moradores de Pedra Branca, uma vez que ameaçam a saúde, os materiais,os cultivos locais e plantas frutíferas. Registrou-se informação sobre a história natural de “besouros” culturalmente importantes. Conclui-se que o conhecimento etnoentomológico dos moradores de Pedra Branca sobre besouros é importante como uma fonte de informação sobre as espécies locais.Fieldwork was carried out from April to December 2004. Data were obtained from 49 individuals (20 men and 29 women by means of open-ended tape-recorded interviews and following an emicist approach. Results show that the generic taxon “beetle” is used as an ethnotaxonomic category that brings together not systematically related organisms, besides the Coleoptera of the Linnaean classification. A total of 43 beetle local names were cited. People have attributed qualitative characteristics to these “beetles”, many of which are anthropomorphic. “Beetles” cause damages to human beings since they harm their health, materials, and local crops and fructiferous plants. Information on the natural history of culturally significant “beetles” was recorded. It is concluded that

  4. Notes on three braconid wasps (Hymenoptera: Braconidae, Doryctinae) parasitizing oak long-horned beetle, Massicus raddei (Coleoptera: Cerambycidae), a severe pest of Quercus spp. in China, together with the description of a new species.

    Science.gov (United States)

    Cao, Liangming; Yang, Zhongqi; Tang, Yanlong; Wang, Xiaoyi

    2015-01-01

    Three species of Doryctinae (Hymenoptera: Braconidae) parasitize larvae of oak longhorn beetle Massicus raddei Blessig (Coleoptera: Cerambycidae), a serious wood borer pest in North China. Rhoptrocentrus quercusi sp. nov., is described as a new species and Doryctes petiolatus Shestakov, as well as Zombrus bicolor (Enderlein). The three species are idiobiont ectoparasitoids, and may have potential for biological control of oak longhorn beetle. PMID:26624143

  5. Rozšíření rýhovců Rhysodes sulcatus (Fabricius, 1787) a Omoglymmius germari (Ganglbauer, 1892) (Coleoptera: Rhysodidae) v České republice

    Czech Academy of Sciences Publication Activity Database

    Konvička, Ondřej; Čížek, Lukáš

    2015-01-01

    Roč. 6, Sept 1 (2015), s. 111-114. ISSN 1804-2732 R&D Projects: GA ČR GAP504/12/1952 Institutional support: RVO:60077344 Keywords : Coleoptera * distributional maps * faunistics Subject RIV: EH - Ecology, Behaviour

  6. Efecto de la dieta artificial MP sobre la emergencia y relacion de sexos de Phymastichus coffea (Hymenoptera:Eulophidae) mantenido sobre su hueped, Hypothenemus hampei (Coleoptera: Scloytidae)a traves de generaciones contin

    Science.gov (United States)

    Phymastichus coffea La Salle (Hymenoptera: Eulophidae) is an endoparasitoid that attacks the adult coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Scolytidae). The MP diet developed by Portilla and Streett is the only reported diet that allows cultures of P. coffea to develop and repr...

  7. Prediction Of Pest Pressure on Corn Root Nodes – The POPP-Corn model

    OpenAIRE

    Agatz, Annika; Ashauer, Roman; Sweeney, Paul; Brown, Colin David

    2016-01-01

    A model for the corn rootworm Diabrotica spp. (Coleoptera: Chrysomelidae) combined with a temporally-explicit model for development of corn roots across the soil profile was developed to link pest ecology, root damage and yield loss. Development of the POPP-Corn model focused on simulating root damage from rootworm feeding in accordance with empirical observations in the field to allow the virtual testing of efficacy from management interventions in the future. Here we present the model and d...

  8. A contribution to the tachinid flies of the subfamilies Exoristinae and Tachininae (Diptera: Tachinidae) from Fars province, Iran

    OpenAIRE

    GHEIBI, Mehdi; Ostovan,Hadi; KAMALI, KARIM

    2010-01-01

    Data are given on the distribution of 40 species belonging to the subfamilies Exoristinae and Tachininae that were collected by the first author in Fars province, Iran, during 2006-2007. In all, 22 species were recorded for the first time from Iran and 34 species from Fars province. Erynniopsis antennata (Rondani, 1861) was reared for the first time on the host Diorhabda elongate (Coleoptera: Chrysomelidae).

  9. The key to success: host plant adaptations in the root herbivore 'Diabrotica virgifera virgifera'

    OpenAIRE

    Robert, Christelle A.M; Turlings, Ted C.

    2012-01-01

    Antagonistic interactions between plants and insects are likely the drivers of a fascinating coevolutionary arms race between the two trophic levels. Plants- and plant breeders- are continuously developing traits that allow them to fend-off herbivores, while phytophagous insect keep inventing counter-adaptations to withstand plant defenses. Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) is a specialist root herbivore of maize, Zea mays. Known as the billion dollar bug in ...

  10. Influence of Host Origin on Host Choice of the Parasitoid Dinarmus basalis: Does Upbringing Influence Choices Later in Life?

    OpenAIRE

    Sankara, F.; Dabiré, L. C. B.; Ilboudo, Z.; Dugravot, S.; Cortesero, A.M.; Sanon, A

    2014-01-01

    The aim of this study was to investigate the influence of volatile compounds from four secondary host plants on the ability of Dinarmus basalis Rond. (Hymenoptera: Pteromalidae) to locate, recognize, and parasitize its host, 4th instar larvae or pupae of Callosobruchus maculatus F. (Coleoptera: Chrysomelidae). To examine this, strains of D. basalis were transferred from cowpea seeds (Vigna unguiculata (L.) Walp. (Fabales: Fabaceae)) to pigeon pea (Cajanus cajan (L.) Millsp.) and two varieties...

  11. Herbivore Larval Development at Low Springtime Temperatures: The Importance of Short Periods of Heating in the Field

    OpenAIRE

    Esther Müller; Elisabeth Obermaier

    2012-01-01

    Temperature has been shown to play an important role in the life cycles of insects. Early season feeders in Palaearctic regions profit by the high nutritional quality of their host plants early in the year, but face the problem of having to develop at low average springtime temperatures. This study examines the influence of short periods of heating in the field on larval development and on mortality with the model system Galeruca tanaceti L. (Coleoptera: Chrysomelidae), an early season feeder...

  12. Fatores que afetam artrópodes associados a cinco acessos de ginseng-brasileiro (Pfaffia glomerata) em Montes Claros, Estado de Minas Gerais = Factors affecting arthropods associated with five accessions of Brazilian ginseng (Pfaffia glomerata) in Montes Claros, Brazil

    OpenAIRE

    Germano Leão Demolin Leite; Mayra Pimenta; Péricles Leonardo Fernandes; Ronnie Von Santos Veloso; Ernane Ronnie Martins

    2008-01-01

    O objetivo deste trabalho foi identificar artrópodes associados a cinco acessos ('NDS', 'COVB', 'NAT', 'ROST' e 'GSD1') de Pfaffia glomerata, bem como o efeito de clima, dossel, face foliar, tricomas e inimigos naturais sobre a entomofauna. Dos artrópodes observados, Aphis gossypii (Hemiptera: Aphididae), Diabrotica speciosa (Coleoptera: Chrysomelidae) e Tetranychus ludeni (Acari: Tetranychidae) apresentaram maior densidade populacional. Dos inimigos naturais observados, ácaros predadores est...

  13. First Record Of Clytra Laeviuscula Ratzeburg As Potential Insect Pest Of Energy Willow (Salix Viminalis L.) In Ukraine

    OpenAIRE

    Stefanovska Tatyana; Lewis Edwin; Pidlisnyuk Valentina; Smyrnykh Oleg

    2015-01-01

    Cultivation of short rotation coppice energy willow (SRC EW), Salix viminalis L., has a great potential in Ukraine as a source of biomass for biofuel production. Commercial production of this species was recently initiated in the country. The growing of SRC EW in Western and Northern Europe for a long time showed that leaf beetles (Coleoptera: Chrysomelidae) are key pests causing significant biomass reduction. However, data about the pest complex for energy willow growing in Ukraine is not av...

  14. Influence of phenolglucosides and trichome density on the distribution of insects herbivores on willows

    OpenAIRE

    Soetens, Ph.; Rowell-Rahier, Martine; Pasteels, Jacques M.

    2009-01-01

    The effects of both trichome density and phenolglucoside content of leaves of 76 willow hybrids (Salix alba x fragilis) were measured to estimate their influence on the distribution of Phratora vitellinae (L.), Plagiodera versicolora Baly (Coleoptera: Chrysomelidae) and Pontania proxima (Lepeletier 1823) (Hymenoptera: Tenthredinidae) in a nursery at Gramont, Belgium. The willows showed differences in their phenolglucoside content and pilosity of leaves and are classified on these basis into ...

  15. Field parasitism of nontarget weevil species (Coleoptera: Curculionidae) by the introduced biological control agent Microctonus aethiopoides Loan (Hymenoptera: Braconidae) over an altitude gradient.

    Science.gov (United States)

    Barratt, B I P; Ferguson, C M; Bixley, A S; Crook, K E; Barton, D M; Johnstone, P D

    2007-08-01

    The parasitoid, Microctonus aethiopoides Loan (Hymenoptera: Braconidae) was introduced into New Zealand in 1982 to control the alfalfa pest, Sitona discoideus Gyllenhal (Coleoptera: Curculionidae). Studies have shown that a number of nontarget weevil species are attacked in the field by this parasitoid. A field study was carried out to investigate nontarget parasitism by M. aethiopoides over an altitudinal sequence from the target host habitat (alfalfa) into native grassland. Three locations were selected for the study, and at each, the alfalfa growing in the valley floor was sampled annually for parasitism of the target pest that ranged between 17 and 78%. At progressively higher altitudes, three further grassland sites at each location were sampled monthly during spring to autumn for up to 6 yr. Weevil densities were estimated, species identified, and dissections carried out to determine reproductive status and parasitism. Almost 12,000 weevils were collected during the study, which were identified as 36 species in total from the three locations. Eight weevil species were found to be parasitized, including S. discoideus, the target host that was found at all sites. Parasitism of nontarget species was approximately 2% overall but varied with location, site, and season. Substantial nontarget parasitism was found at only one of the locations, with up to 24% parasitism of a native weevil, Nicaeana fraudator Broun (Coleoptera: Curculionidae), recorded. Another species, Irenimus egens (Broun) (Coleoptera: Curculionidae), was also found at this location at similar population densities but was attacked far less by M. aethiopoides. Results are discussed in relation to weevil phenology. PMID:17716473

  16. Aspectos biológicos e técnica de criação do gorgulho-da-goiaba, Conotrachelus psidii marshall (Coleoptera: Curculionidae)

    OpenAIRE

    Fabricio Iglesias Valente; Vera Lúcia Rodrigues Machado Benassi

    2014-01-01

    Considerada praga-chave da cultura da goiaba, Conotrachelus psidii Marshall (Coleoptera: Curculionidae) tem causado grandes prejuízos em vários países produtores desta fruta. A pesquisa objetivou avaliar os parâmetros biológicos da praga, sob condições controladas, através do desenvolvimento de uma técnica de criação que permitiu efetuar observações diárias de pré-pupas e pupas. Constatou-se que o substrato adequado para manutenção dessas fases foi o solo argiloso umedecido com viabilidade de...

  17. Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae

    Directory of Open Access Journals (Sweden)

    Juan Pablo Botero

    2013-12-01

    Full Text Available Description of the male of Eburella pinima Martins and notes on the geographical distribution of Eburodacrys aenigma Galileo & Martins and Eburodacrys lanei Zajciw (Coleoptera, Cerambycidae. The male of Eburella pinima Martins, 1997 is described and illustrated for the first time. Information on Eburodacrys aenigma Galileo & Martins, 2006, previously known only from the female holotype, which lacked locality label, is herein complemented. This species is recorded from Brazil and the male is depicted for the first time. The geographical distribution of Eburodacrys lanei Zajciw, 1958 is further restricted here as some previous records are confirmed to result from misidentifications of E. aenigma.

  18. The infestation by an exotic ambrosia beetle, Euplatypus parallelus (F. (Coleoptera: Curculionidae: Platypodinae of Angsana trees (Pterocarpus indicus Willd. in southern Thailand

    Directory of Open Access Journals (Sweden)

    Sara Bumrungsri

    2008-07-01

    Full Text Available An exotic ambrosia beetle, Euplatypus parallelus (F. was collected from infested Pterocarpus indicus Willd. trees in Prince of Songkla University. Larvae and eggs were found in simple galleries with a single branch. Either a single male or a male and a female were found in each gallery. Half of these infested trees were previously attacked by long-horned beetles probably Aristobia horridula (Hope (Coleoptera: Cerambycidae, while some of them appeared to be healthy. Fusarium oxysporum Schlecht.:Fr. was isolated from frass, sapwood samples and insect larvae, and might be a cause of death of P.indicus.

  19. A new species of endogean, anophthalmous Parazuphium Jeannel from Northern Morocco (Coleoptera: Carabidae, with new molecular data for the tribe Zuphiini

    Directory of Open Access Journals (Sweden)

    Carmelo Andújar

    2011-06-01

    Full Text Available A new species of the genus Parazuphium (Coleoptera, Carabidae, Zuphiini, Parazuphium aguilerai sp.n., is described from the Tingitan peninsula in North Morocco. The single known specimen was found below a large stone deeply inserted in the substratum, and it is anophthalmous, depigmented and flattened. This is the second species of blind Parazuphium, the other being P. feloi Machado 1998 from a lave tube in the Canary Islands. Molecular data of the unique known P. aguilerai sp.n. specimen is provided, and a molecular phylogeny confirm its inclusion inside Zuphiini within Harpalinae. Identification keys to the Mediterranean and Macaronesian species of Parazuphium are provided.

  20. Estudio taxonómico de las especies de la familia bruchidae (Insecta: Coleoptera) relacionadas con el género Desmodium (Fabaceae) en el mundo.

    OpenAIRE

    Vásquez Chan, Adriano

    2011-01-01

    La presente investigación se realizó con el propósito de determinar las especies de brúquidos (Coleoptera: Bruchidae) que se alimentan de semillas de plantas del género Desmodium Desv. (Leguminosae: Papilionoideae) en el mundo. Se desarrollaron claves dicotómicas para determinar géneros y especies de brúquidos del Nuevo y Viejo Mundo, además de ampliar la información existente sobre la asociación brúquido-Desmodium de la base de datos BRUCOL. Se revisaron semillas de Desmodium infestadas por ...

  1. Efeitos da radiação gama do Cobalto-60 em ovos de Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae Gamma radiation effects on eggs of Tribolium castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae

    Directory of Open Access Journals (Sweden)

    L. S. Fontes

    1994-12-01

    Full Text Available Este trabalho teve por objetivo determinar as doses esterilizantes e letal para ovos de T. castaneum (Herbst., 1797 (Coleoptera: Tenebrionidae, através de doses crescentes de radiação gama. Utilizou-se uma fonte de Cobalto-60, tipo Gammabeam-650, com taxa de dose de 1,28 kGy/hora. O experimento foi conduzido sob condições controladas com temperatura de 25 ± 2°C e umidade relativa de 70 ± 5%. As doses letal e esterilizante para os ovos foram respectivamente 30 e 20 Gy.The objective of this research was to verify the effects of gamma radiation of a Cobalt-60 source on eggs of the red flour beetle Tribolium castaneum (Herbst., 1797. The used dose rate was 1.28 kGy per hour, and the irradiated insects were kept under controlled environment condition: 25 ± 2°C and 70 ± 5% relative humidity. For eggs the sterilizing and lethal dose were respectively, 20 and 30 Gy.

  2. Los ejemplares tipo de Dryopidae, Elmidae, Limnichidae y Psephenidae (Coleoptera: Byrrhoidea, depositados en la colección del Museo de La Plata The types of Dryopidae, Elmidae, Limnichidae and Psephenidae (Coleoptera: Byrrhoidea housed at the Museo de La Plata

    Directory of Open Access Journals (Sweden)

    Liliana A. Fernández

    2004-07-01

    Full Text Available Se examinaron los ejemplares tipo depositados en la colección de la División Entomología del Museo de La Plata, pertenecientes a diversas especies nominales de Byrrhoidea: Dryopidae (1 especie, 1 ejemplar tipo, Elmidae (4, 9, Limnichidae (29, 47 y Psephenidae (1, 2. El material tipo fue estudiado por los siguientes autores: Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman y Wooldridge. Para cada taxón se indica la publicación original, datos de colección y condición del material.The types of species of Byrrhoidea (Coleoptera housed in the collection of the División Entomología of Museo de La Plata were examined and listed. They belong to 35 species distributed in the families Dryopidae (1 species, 1 type, Elmidae (4, 9, Limnichidae (29, 47 and Psephenidae (1, 2. The types were described by Pic, Spangler, Spangler & Brown, Spangler & Perkins, Spangler & Santiago, Spilman, and Wooldridge. For each taxon, complete information about categories of types, reference original descriptions, collection data and specimen condition are given.

  3. Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, MS, Brazil

    Directory of Open Access Journals (Sweden)

    Sergio Roberto Rodrigues

    2012-09-01

    Full Text Available Occurrence of adults and biological aspects of Geniates borelli Camerano (Coleoptera, Scarabaeidae, Rutelinae in Aquidauana, Mato Grosso do Sul, Brazil. Due to the importance of Geniates borelli Camerano as a pest in many crops, studies were developed at Universidade Estadual de Mato Grosso do Sul, Aquidauana campus, MS, Brazil. Adults were collected with a light trap from January 2006 to December 2007. 3,320 adults were collected, and the highest quantities were obtained in October 2006 and November 2007, with 1,548 and 802 adults recorded, respectively. Collected adults were kept in plastic containers with soil and Brachiaria decumbens seedlings for oviposition. 535 eggs measuring 2.30 × 1.60 mm were obtained. As the embryonic development progressed, eggs increased in size to 3.00 × 2.70 mm, and this change occurred between 6 and 10 days after oviposition. The embryonic period lasted 17.9 days. The first, second, and third instars lasted 37.6, 49.7, and 74 days, respectively. The prepupal stage lasted 65.9 days and the pupal stage lasted an average of 18.5 days. The biological cycle is completed in 315.8 days, which characterizes the species as univoltine. The average longevity of females was 35.4 days and 28.5 days for males.

  4. Updated distribution of Osmoderma eremita in Abruzzo (Italy and agro-pastoral practices affecting its conservation (Coleoptera: Scarabaeidae

    Directory of Open Access Journals (Sweden)

    Patrizia Giangregorio

    2015-12-01

    Full Text Available New records of Osmoderma eremita (Scopoli, 1763 (Coleoptera: Scarabaeidae: Cetoniinae are reported for Abruzzo (Italy, together with a review of its distribution in this region. O. eremita is a saproxylic beetle dependent on the presence of hollow deciduous trees with abundant wood mould in their cavities. The major threats for the species are habitat loss and fragmentation. EU Habitats Directive requests to the member States its protection and the monitoring of its conservation status. Detection of its occurrence is the first step to protect the species. The surveys have been carried out in ten sites of Abruzzo by using black cross-windows traps baited with specific pheromone. The species has been recorded for the first time in the Sant’Antonio forest and its presence is confirmed in the Peligna Valley, after a decade. The populations seem to be confined to small patches of suitable habitats. At local level, the abandonment of the pollarding practice (willow and beech forests and the use of pollarded trees as biomass for fuel are the major threats for this species. Indeed some key actions, such as the protection of old hollow trees and the continuation of pollarding practice in rural landscape, could be key factors for the conservation strategies of the species in the study area.

  5. Population genetics and phylogenetic relationships of beetles (Coleoptera: Histeridae and Staphylinidae) from the Sonoran Desert associated with rotting columnar cacti.

    Science.gov (United States)

    Pfeiler, Edward; Johnson, Sarah; Richmond, Maxi Polihronakis; Markow, Therese A

    2013-12-01

    Dozens of arthropod species are known to feed and breed in the necrotic tissues (rots) of columnar cacti in the Sonoran Desert. Because the necrotic patches are ephemeral, the associated arthropods must continually disperse to new cacti and therefore the populations of any given species are expected to show very little local genetic differentiation. While this has been found to be true for the cactophilic Drosophila, the evolutionary histories and characteristics of other arthropods inhabiting the same necrotic patches, especially the beetles, have yet to be examined. Here we used nucleotide sequence data from segments of the mitochondrial 16S rRNA and cytochrome c oxidase subunit I (COI) genes to examine population structure and demographic history of three sympatric beetle species (Coleoptera: Histeridae and Staphylinidae) collected on senita cactus (Lophocereus schottii) from six widely-separated localities on the Baja California peninsula of northwestern Mexico. Two histerids, Iliotona beyeri and Carcinops gilensis, and an unidentified staphylinid, Belonuchus sp., showed little or no population structure over a broad geographic area on the peninsula, consistent with the prediction that these beetles should show high dispersal ability. Demographic tests revealed varying levels of historical population expansion among the beetle species analyzed, which are discussed in light of their ecologies and concurrent biogeographic events. Additionally, phylogenetic analyses of COI sequences in Carcinops collected on a variety of columnar cacti from both peninsular and mainland Mexico localities revealed several species-level partitions, including a putative undescribed peninsular species that occurred sympatrically with C. gilensis on senita. PMID:23948866

  6. Incidence of turf-damaging white grubs (Coleoptera: Scarabaeidae) and associated pathogens and parasitoids on Kentucky golf courses.

    Science.gov (United States)

    Redmond, Carl T; Potter, Daniel A

    2010-12-01

    Root-feeding grubs (Coleoptera: Scarabaeidae) were sampled from damaged areas of 61 irrigated roughs on 32 Kentucky golf courses to determine species composition and natural enemy incidence, the first such survey in the United States' transitional turfgrass climatic zone. Masked chafers (Cyclocephala lurida Bland and C. borealis Arrow) and Japanese beetle (Popillia japonica Newman) accounted for ≈73 and 26% of grubs found in an autumn survey, with Cyclocephala spp. predominating at most sites, although mixed infestations were common. Only a few Phyllophaga spp., and no exotic species other than P. japonica were found. Cyclocephala spp. also predominated in seasonal and statewide surveys regardless of whether a course had cool- or warm-season grass fairways. Pathogenic bacteria, Paenibacillus and Serratia spp., and the autumn-active parasitoid Tiphia pygidialis Allen were the main enemies associated with Cyclocephala spp. Predominant enemies of P. japonica were Paenibacillus, Serratia, and Metarhizium spp. in autumn, and eugregarines, Stictospora sp. (probably S. villani Hays and Clopton) and Tiphia vernalis Rohwer in spring. Entomopathogenic nematodes and the microsporidian Ovavesicula popilliae Andreadis & Hanula were nearly absent in our samples. No predictive relationships were found between soil parameters and proportionate abundance of Cyclocephala or P. japonica, or with natural enemy incidence at particular sites. Although incidence of individual enemies was generally low (hosts' prolonged development they may take a significant toll on grub populations. PMID:22182549

  7. Attraction of Tomicus yunnanensis (Coleoptera: Scolytidae to Yunnan Pine Logs with and without Periderm or Phloem: An Effective Monitoring Bait

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    Rong Chun Lu

    2012-01-01

    Full Text Available The Yunnan pine shoot beetle, Tomicus yunnanensis Kirkendall and Faccoli (Coleoptera: Scolytinae is an important pest of Yunnan pine (Pinus yunnanensis Franch in China. Experiments with host log baits were done to develop a pest monitoring system using host tree kairomone. Five Yunnan pine logs (each 10–15 cm diam. × 30-cm long in a trap-log bundle were treated by peeling periderm (outer bark off to expose the phloem, and half of each log was covered with sticky adhesive to capture any attracted adult beetles. Significantly, more beetles were attracted and caught on the periderm-peeled logs (ca 30 beetles/m2 log surface/day than on untreated control logs with adhesive (ca 2.5/m2/day. No significant differences were observed between catches on logs taken from lower or upper halves of Yunnan pines. T. yunnanensis flies mostly during the afternoon according to trap catches throughout the day. Attraction to the periderm-peeled logs decreased considerably when they were peeled further to remove the phloem, indicating phloem volatiles play a role in selection of the host by the beetle. The readily-available log baits appear useful for monitoring pine shoot beetle populations in integrated pest management programs.

  8. Morfología e histología del sistema digestivo del burrito del frambueso Aegorhinus superciliosus (Coleoptera: Curculionidae

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    VIVIAN MEDEL

    2013-12-01

    Full Text Available Se realizó el estudio morfológico e histológico del sistema digestivo de Aegorhinus superciliosus (Coleoptera: Curculionidae, plaga de importancia económica en el sur de Chile. Esta especie presenta un sistema digestivo característico para la mayoría de los insectos, siendo similar en machos y hembras, pero de mayor longitud en las últimas, llegando a medir el doble del tamaño del insecto. El sistema digestivo es un tubo largo de diámetro variable, dividido en tres regiones: intestino anterior (estomodeo, intestino medio (mesenterón e intestino posterior (proctodeo. Histológicamente presenta un tejido epitelial similar al descrito para otras especies y externamente un tejido muscular, sin diferencias entre machos y hembras. No obstante, presenta características que difieren en otros curculiónidos y coleópteros tales como: ausencia de buche, ubicación y número de los ciegos gástricos, presencia de membrana peritrófica y ausencia de almohadillas rectales.

  9. Occurrence and biology of Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae) parasitising different species of Coccinellidae (Coleoptera) in Neotropical region.

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    Silva, R B; Cruz, I; Figueiredo, M L C; Pereira, A G; Penteado-Dias, A M

    2012-02-01

    Surveys on Coccinellidae (Coleoptera) in Sete Lagoas city, Minas Gerais state, Brazil, indicated the parasitism of adults of the species Coleomegilla maculata De Geer, 1775, Eriopis connexa (Germar, 1824) and Olla v-nigrum (Mulsant, 1866), by Dinocampus coccinellae (Schrank, 1802) (Hymenoptera; Braconidae: Euphorinae). Since then, the parasitoid have been maintained in its original hosts at the Insect Rearing Laboratory - LACRI of the Brazilian Agricultural Research Institution - Embrapa Milho e Sorgo. Besides the citation of occurrence in Brazil, this work also indicates the parasitoid preference for C. maculata (70% of parasitism), followed by O. v-nigrum (43.3% of parasitism) and E. connexa (36.7% of parasitism). Total life cycle of D. coccinellae was longer on C. maculata (32.4 ± 0.48 days), compared to O. v-nigrum (29.5 ± 0.49 days) and E. connexa (27.8 ± 0.4 days). Due to the relatively high percentage of field parasitism, D. coccinellae can reduce the efficiency of biological pest control by Coccinellidae predators especially in the case of C. maculata. PMID:22437405

  10. A DNA barcode library of the beetle reference collection (Insecta: Coleoptera in the National Science Museum, Korea

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    Sang Woo Jung

    2016-06-01

    Full Text Available Coleoptera is a group of insects that are most diverse among insect resources. Although used as indicator species and applied in developing new drugs, it is difficult to identify them quickly. Since the development of a method using mitochondrial DNA information for identification, studies have been conducted in Korea to swiftly and accurately identify species. The National Science Museum of Korea (NSMK has been collecting and morphologically identifying domestic reference insects since 2013, and building a database of DNA barcodes with digital images. The NSMK completed construction of a database of digital images and DNA barcodes of 60 beetle species in the Korean National Research Information System. A total of 179 specimens and 60 species were used for the analysis, and the averages of intraspecific and interspecific variations were 0.70±0.45% and 26.34±6.01%, respectively, with variation rates ranging from 0% to 1.45% and 9.83% to 56.23%, respectively.

  11. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini)

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    Miller, Kelly B.

    2016-01-01

    Abstract The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps.

  12. Knockdown, residual, and antifeedant activity of pyrethroids and home landscape bioinsecticides against Japanese beetles (Coleoptera: Scarabaeidae) on Linden foliage.

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    Baumler, Rebecca E; Potter, Daniel A

    2007-04-01

    Residual toxicity and leaf protection capability of five pyrethroids, professional and home garden azadirachtin formulations, and six other bioinsecticides for the home landscape were evaluated against the Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), on linden, Tilia cordata L. Capacity of intoxicated beetles to recover and subsequently feed and disperse also was evaluated to provide insight on activity characteristics of the different compounds. Intact shoots were sprayed and left in the field for varying intervals before being challenged with beetles in no-choice and choice tests. All pyrethroids except permethrin gave greater leaf protection, knockdown, and kill than did carbaryl, the standard, after 14 d of weathering. Deltamethrin, cyfluthrin, bifenthrin, and lamda-cyhalothrin gave a high level of protection for at least 19 d, and azadirachtin (Azatin XL) deterred feeding in choice tests for as long as 14 d. Home garden formulations containing pyrethrins in canola oil (Pyola) or azadiractin (Neem-Away) gave good short-term (< 3-d) protection. Formulations of capsaicin, rotenone + pyrethrins, kaolin particle film, D-limonene, or garlic extract were ineffective, the latter two formulations being highly phytotoxic to linden. Results of this study should help support updating of guidelines for insecticidal control of Japanese beetles. PMID:17461070

  13. Biology and host preferences of Cryptorhynchus melastomae (Coleoptera: Curculionidae), a possible biocontrol agent for Miconia calvescens (Melastomataceae) in Hawaii.

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    Reichert, Elisabeth; Johnson, M Tracy; Chacón, Eduardo; Anderson, Robert S; Wheeler, Terry A

    2010-12-01

    The introduced plant Miconia calvescens (Melastomataceae) poses a grave threat to Hawaii's native ecosystems and biodiversity. One potential candidate for classical biological control is Cryptorhynchus melastomae (Coleoptera: Curculionidae: Cryptorhynchinae), a stem-boring weevil from Central and South America. This weevil feeds on M. calvescens in its native Costa Rica and has been successfully reared under greenhouse conditions. Comparison of its environmental conditions in Costa Rica with those in the Miconia infested areas of Hawaii indicates the latter is a suitable habitat for C. melastomae. C. melastomae has one or two generations per year. Adults feed on new stems, petioles, leaf buds, veins, and lamina, whereas larvae mine the stem until pupation. Adults appear to prefer saplings for oviposition and feeding. Under greenhouse conditions both adults and larvae can seriously damage and kill small M. calvescens. Preliminary host testing indicates that C. melastomae may be family specific on Melastomataceae. However, because Hawaii lacks native melastomes and has many other serious melastome weeds, a family specific insect may be suitable as a biocontrol agent in this case. PMID:22182550

  14. Acute toxicity of plant essential oils to scarab larvae (Coleoptera: Scarabaeidae) and their analysis by gas chromatography-mass spectrometry.

    Science.gov (United States)

    Ranger, Christopher M; Reding, Michael E; Oliver, Jason B; Moyseenko, James J; Youssef, Nadeer; Krause, Charles R

    2013-02-01

    Larvae of scarab beetles (Coleoptera: Scarabaeidae) are important contaminant and root-herbivore pests of ornamental crops. To develop alternatives to conventional insecticides, 24 plant-based essential oils were tested for their acute toxicity against third instars of the Japanese beetle Popillia japonica Newman, European chafer Rhizotrogus majalis (Razoumowsky), oriental beetle Anomala orientalis (Waterhouse), and northern masked chafer Cyclocephala borealis Arrow. Diluted solutions were topically applied to the thorax, which allowed for calculating LD50 and LD90 values associated with 1 d after treatment. A wide range in acute toxicity was observed across all four scarab species. Of the 24 oils tested, allyl isothiocyanate, cinnamon leaf, clove, garlic, and red thyme oils exhibited toxicity to all four species. Allyl isothiocyanate was the most toxic oil tested against the European chafer, and among the most toxic against the Japanese beetle, oriental beetle, and northern masked chafer. Red thyme was also comparatively toxic to the Japanese beetle, oriental beetle, European chafer, and northern masked chafer. Interspecific variability in susceptibility to the essential oils was documented, with 12, 11, 8, and 6 of the 24 essential oils being toxic to the oriental beetle, Japanese beetle, European chafer, and northern masked chafer, respectively. Analysis of the active oils by gas chromatography-mass spectrometry revealed a diverse array of compounds, mostly consisting of mono- and sesquiterpenes. These results will aid in identifying active oils and their constituents for optimizing the development of plant essential oil mixtures for use against scarab larvae. PMID:23448028

  15. Revision of the Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera, Dytiscidae, Hydroporinae, Bidessini).

    Science.gov (United States)

    Miller, Kelly B

    2016-01-01

    The Neotropical diving beetle genus Hydrodessus J. Balfour-Browne, 1953 (Coleoptera: Dytiscidae: Hydroporinae: Bidessini) is revised. Thirty species are recognized. The following new species are described: Hydrodessus bimaculatus sp. n. (Venezuela), Hydrodessus brevis sp. n. (Venezuela), Hydrodessus concolorans sp. n. (Venezuela), Hydrodessus continuus sp. n. (Venezuela), Hydrodessus disjunctus sp. n. (Suriname), Hydrodessus fasciatus sp. n. (Brazil), Hydrodessus imparilis sp. n. (Ecuador), Hydrodessus keithi sp. n. (Brazil, Colombia, Ecuador), Hydrodessus kurti sp. n. (Suriname), Hydrodessus kylei sp. n. (Suriname, Venezuela), Hydrodessus laetus sp. n. (Venezuela), Hydrodessus latotibialis sp. n. (Peru), Hydrodessus maculatus sp. n. (Guyana, Venezuela), Hydrodessus morsus sp. n. (Venezuela), Hydrodessus palus sp. n. (Venezuela), and Hydrodessus tenuatus sp. n. (Suriname). The following new synonyms are established: Hydrodessus fragrans Spangler, 1985 = Hydrodessus biguttatus (Guignot, 1957) syn. n. and Hydrodessus robinae Spangler, 1985 = Hydrodessus octospilus (Guignot, 1957), syn. n. One species is transferred from Hydrodessus to Amarodytes Régimbart, Amarodytes soekhnandanae (Makhan, 1994), comb. n. Habitus photographs (dorsal and lateral) and photos of the ventral surfaces are provided for most species. Line drawings of male and female genitalia and other diagnostic features are also provided along with distribution maps. PMID:27110208

  16. Repellent Activity of the Essential Oil from the Heartwood of Pilgerodendron uviferum (D. Don) Florin against Aegorhinus superciliosus (Coleoptera: Curculionidae).

    Science.gov (United States)

    Espinoza, Javier; Urzúa, Alejandro; Tampe, Jocelyne; Parra, Leonardo; Quiroz, Andrés

    2016-01-01

    The weevil Aegorhinus superciliosus Guérin (Coleoptera: Curculionidae), which is endemic to Central-Southern Chile and Argentina, is one of the major berry pests in Chile and the most important pest in the La Araucanía Region (38°44'9″S, 72°35'25″W). Due to the poor effectiveness and problems surrounding the implementation of the traditional control methods using organophosphate and carbamate insecticides, new strategies for controlling this pest are needed. In this communication, we evaluated the behavioral responses of male and female A. superciliosus to volatile compounds released from the essential oil (EO) obtained from the heartwood of Pilgerodendron uviferum (D. Don) Florin using olfactometric bioassays. The composition of the EO was analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). According to these analyses, δ-cadinol (24.16%), cubenol (22.64%), 15-copaenol (15.46%) and δ-cadinene (10.81%) were the principal components of the EO. The Pilgerodendron uviferum EO, which is almost exclusively composed of sesquiterpenes (99.5%), exhibited a repellent effect against A. superciliosus adults, regardless of the sex or concentration used (56.6 mg/cm³ and 1.58 × 10(-2) mg/cm³). The EO has low volatility and greater persistence than the EOs composed of monoterpenes and is considered a good model in the search for raspberry weevil repellents. PMID:27110756

  17. Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864

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    Ubirajara R. Martins

    2006-01-01

    Full Text Available Calliini (Coleoptera, Cerambycidae, Lamiinae: descrições, homonímia, novo registro e chave para as espécies de Graminea Thomson, 1864. Espécies novas descritas: Paracallia giesberti sp. nov. da Bolívia (Cochabamba, Colombicallia albofasciata sp. nov. e Graminea rubra sp. nov. da Bolívia (Santa Cruz; Asemolea flava sp. nov. da Costa Rica, (Heredia; Callia potiaiuba sp. nov. do Brasil (Rondônia e da Bolívia (Cochabamba e Santa Cruz. É proposto o nome Rumuara nom. nov. para Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Apresenta-se novo registro para a Bolívia para Eumimesis trilineata Magno & Monné, 1990. Acrescenta-se chave para as espécies de Graminea.Calliini (Coleoptera, Cerambycidae, Lamiinae: descriptions, homonym, new record and key to the species of Graminea Thomson,1864. New species described: Paracallia giesberti sp. nov. from Bolivia (Cochabamba, Colombicallia albofasciata sp. nov. and Graminea rubra sp. nov. from Bolivia (Santa Cruz; Asemolea flava sp. nov. from Costa Rica, (Heredia; Callia potiaiuba sp. nov. from Brazil (Rondônia and Bolivia (Cochabamba and Santa Cruz. Rumuara nom. nov. is proposed for Paracladus Martins & Galileo, 1990 non Carpenter, 1966 (Orthoptera. Eumimesis trilineata Magno & Monné, 1990 is recorded for Bolivia. A key to the species of Graminea is added.

  18. Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis.

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    Schmidt, Jason M; Szendrei, Zsofia; Grieshop, Matthew

    2016-01-01

    Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), plum curculio, is a serious direct pest of North American tree fruit including, apples, cherries, peaches and plums. Historically, organophosphate insecticides were used for control, but this tool is no longer registered for use in tree fruit. In addition, few organically approved insecticides are available for organic pest control and none have proven efficacy as this time. Therefore, promoting biological control in these systems is the next step, however, little is known about the biological control pathways in this system and how these are influenced by current mechanical and cultural practices required in organic systems. We used molecular gut-content analysis for testing field caught predators for feeding on plum curculio. During the study we monitored populations of plum curculio and the predator community in a production organic apple orchard. Predator populations varied over the season and contained a diverse assemblage of spiders and beetles. A total of 8% of all predators (eight Araneae, two Hemiptera, and six Coleoptera species) assayed for plum curculio predation were observed positive for the presence of plum curculio DNA in their guts, indicating that these species fed on plum curculio prior to collection Results indicate a number of biological control agents exist for this pest and this requires further study in relation to cultural practices. PMID:27348005

  19. Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

    Science.gov (United States)

    Gotoh, Hiroki; Ishiguro, Mai; Nishikawa, Hideto; Morita, Shinichi; Okada, Kensuke; Miyatake, Takahisa; Yaginuma, Toshinobu; Niimi, Teruyuki

    2016-01-01

    Various types of weapon traits found in insect order Coleoptera are known as outstanding examples of sexually selected exaggerated characters. It is known that the sex determination gene doublesex (dsx) plays a significant role in sex-specific expression of weapon traits in various beetles belonging to the superfamily Scarabaeoidea. Although sex-specific weapon traits have evolved independently in various Coleopteran groups, developmental mechanisms of sex-specific expression have not been studied outside of the Scarabaeoidea. In order to test the hypothesis that dsx-dependent sex-specific expression of weapon traits is a general mechanism among the Coleoptera, we have characterized the dsx in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Tenebrionidea, Tenebirionidae). By using molecular cloning, we identified five splicing variants of Gnatocerus cornutus dsx (Gcdsx), which are predicted to code four different isoforms. We found one male-specific variant (GcDsx-M), two female-specific variants (GcDsx-FL and GcDsx-FS) and two non-sex-specific variants (correspond to a single isoform, GcDsx-C). Knockdown of all Dsx isoforms resulted in intersex phenotype both in male and female. Also, knockdown of all female-specific isoforms transformed females to intersex phenotype, while did not affect male phenotype. Our results clearly illustrate the important function of Gcdsx in determining sex-specific trait expression in both sexes. PMID:27404087

  20. Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons, nova sinonímia e novos registros

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    Ubirajara R. Martins

    2007-01-01

    Full Text Available Notas e descrições em Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. Novos táxons e novos registros. Descrição de espécies novas: Zikanita egregia sp. nov. (Bolívia, Santa Cruz; Dufauxia thomasi sp. nov. (Bolívia, Santa Cruz; Irundisaua punctata sp. nov. (Colômbia, Cauca; Cotyzineus gen. nov., espécie-tipo C. bruchi (Melzer, 1931 comb. nov. Novos registros: Zikanita argenteofasciata (Tippmann, 1960 é assinalada para o Brasil (Maranhão e Cotyzineus bruchi para a Bolívia (Santa Cruz. Novo sinônimo: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.Notes and descriptions on Acanthoderini (Coleoptera, Cerambycidae, Lamiinae. I. New taxa and new records. New taxa described: Zikanita egregia, sp. nov. from Bolivia (Santa Cruz; Dufauxia thomasi sp. nov. from Bolivia (Santa Cruz; Irundisaua punctata sp. nov. from Colombia (Cauca; Cotyzineus gen. nov. type species C. bruchi (Melzer, 1931 comb. nov. New records: Zikanita argenteofasciata (Tippmann, 1960 for Brazil (Maranhão; Cotyzineus bruchi for Bolivia (Santa Cruz. New synomym: Ateralphus diringsi Martins & Monné, 1993 = A. javariensis Lane, 1965.