76 FR 21815 - Airworthiness Directives; The Boeing Company Model 737 Airplanes

Science.gov (United States)

2011-04-19

... proposed AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC... and pictures be added to Section 3.B.1.n.(1) of Boeing Alert Service Bulletin 737-27A1277, Revision 1... information directly to the manager of the ACO, send it to the attention of the person identified in the...

78 FR 72564 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-12-03

.../ground relay in the P36 panel versus the location specified in Figure 15, Sheet 2, of Boeing Special... alternate relay locations. Figure 15, Sheet 2, of Boeing Special Attention Service Bulletin 757-24-0132... bundle routing described in Figure 1, Sheet 2, of Boeing Special Attention Service Bulletin 757-24-0132...

Acoustic Emission based on sentry function to monitor the initiation of delamination in composite materials

International Nuclear Information System (INIS)

Bakhtiary Davijani, A.A.; Hajikhani, M.; Ahmadi, M.

2011-01-01

Research highlights: → Constant load does not confirm constant damage in composite materials. → Different damages have different AE events. → Sentry function is a useful tool to monitor the initiation of damage in delamination. → The less sentry function number is the more damage the material has endured. -- Abstract: Delamination is the most common failure mode in composite materials, since it will result in the reduction of stiffness and can grow throughout other layers. Delamination is consisted of two main stages including initiation and propagation. Understanding the behavior of the material in these zones is very important, hence it has been thoroughly studied by different methods such as numerical methods, Acoustic Emission (AE), and modeling. Between these two regions initiation is a more vital stage in the delamination of the material. Once initiation occurs, which normally requires greater amount of force, cracks can easily propagate through the structure with little force and cause the failure of the structure. A better knowledge of initiation can lead to better design and production of stronger materials. Additionally, more knowledge about crack initiation and its internal microevents would help improve other parameters and result in higher strength against crack initiation. AE is a suitable method for in situ monitoring of damage in composite materials. In this study, AE was applied to test different glass/epoxy specimens which were loaded under mode I delamination. A function that combines AE and mechanical information is employed to investigate the initiation of delamination. Scanning electron microscope (SEM) was used to verify the results of this function. It is shown that this method is an appropriate technique to monitor the behavior of the initiation of delamination.

78 FR 66254 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-11-05

... AD. Costs of Compliance We estimate that this AD affects 84 airplanes of U.S. registry. We estimate... inspection cycle. We have received no definitive data that would enable us to provide a cost estimate for the... 2 of paragraph 1.E., ``Compliance,'' of Boeing Service Bulletin 747-53A2688, Revision 1, dated...

78 FR 70211 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-11-25

... Replacement figures of Boeing Special Attention Service Bulletin 757- 57-0071, dated September 12, 2012. The... information identified in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management... Operations, M-30, West Building [[Page 70212

Dissection of the sentry ganglion by laparoscopic boarding in patients with cervix uterine cancer clinical stages IA2 at IIB; Diseccion de ganglio centinela por abordaje laparoscopico en pacientes con cancer cervicouterino etapas clinicas IA2 a IIB

Energy Technology Data Exchange (ETDEWEB)

Valdez U, J J; Pichardo M, P A; Cortes M, G; Escudero de los Rios, P. [Hospital de Oncologia. Centro Medico Nacional Siglo XXI. IMSS, Mexico D.F. (Mexico)

2005-07-01

The obtained results in presently study demonstrate that the feasibility of the detection of the sentry ganglion in cervix uterine cancer using a boarding by laparoscopic via, being necessary the use of twice labelled as much with patent blue and radioisotope (colloid of labelled rhenium with {sup 99m}Tc, total dose of 3 MCi) to achieve the identification of the ganglion. (Author)

78 FR 47549 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-08-06

... Airworthiness Directives; The Boeing Company Airplanes AGENCY: Federal Aviation Administration (FAA), DOT... to certain The Boeing Company Model 737-200, -200C, -300, and -400 series airplanes. AD 2000-06-13 R1..., adds inspections and related investigative and corrective actions, revises certain inspection types...

77 FR 71723 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-12-04

... Airworthiness Directives; The Boeing Company Airplanes AGENCY: Federal Aviation Administration (FAA), DOT... directive (AD) that applies to certain The Boeing Company Model 737-200, -200C, - 300, and -400 series... related investigative and corrective actions, and revise certain inspection types. This proposed AD would...

78 FR 11972 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-02-21

... corresponding figures of Boeing Special Attention Service Bulletin 737-53-1287, Revision 1, dated November 15... service information identified in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE...

77 FR 54798 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-09-06

... that Figures 18 and 23 of Boeing Special Attention Service Bulletin 747-25-3586, dated November 12.... ADDRESSES: For service information identified in this AD, contact Boeing Commercial Airplanes, Attention.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New...

78 FR 9 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-01-02

..., Revision 1, dated March 31, 2005. (ii) At the times specified in Figure 8 of Boeing Special Attention... information identified in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE...

78 FR 33193 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-06-04

... modifications (such as the installation of felt on Model 767 airplanes per Figure 18 of Boeing Special Attention... information identified in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE...

78 FR 25372 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-05-01

... this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P. O. Box 3707, MC... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590... Part 5 of the Accomplishment Instructions and corresponding figures of Boeing Service Bulletin 737-53...

Factors associated to the imposition of types of violence against women informed in sentry services

Directory of Open Access Journals (Sweden)

Luiza Jane Eyre de Souza Vieira

2013-07-01

Full Text Available OBJECTIVE: to identify the prevalence and the factors associated to the imposition of the different types of violence against women informed in sentry services. METHOD: transversal study accomplished through 939 notification forms of cases of violence against women, referring to the three years from 2006 to 2008 in Fortaleza, Ceará. Univariate and multiple analyses by logistic regression were realized. RESULT: the results showed a positive association between the imposition of types of violence against women with a schooling varying from illiterate to basic education and the aggression which had occurred other times. CONCLUSION: this knowledge contributes to the delineation of specific actions that aim at facing this problem, as well as generates subsidies for adequate attendance proposals and guidance for the victims who call on health services.

78 FR 25369 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-05-01

..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590... and corresponding figures of Boeing Service Bulletin 737-53-1310, dated October 20, 2011, are not...

77 FR 16147 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-03-20

... identified in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE... that note (f) of Figures 10 and 24 of Boeing Alert Service Bulletin 767-56A0010, Revision 1, dated...

77 FR 41041 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-07-12

... Clarify Note (d) of Figures 1 and 2 UAL noted that STEP 2, Note (d), of Boeing Special Attention Service... in this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE...

78 FR 65166 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-10-31

..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65... Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590. FOR FURTHER...'' inspections, done in accordance with Part 4 and Figure 8 of Boeing Alert Service Bulletin 747-53A2371...

78 FR 17285 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-03-21

..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590... performing Figures 7 through 10 of Boeing Service Bulletin 767-27A0224, Revision 1, dated December 16, 2011...

77 FR 2669 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-01-19

...'' column of Table 1 of Figure 1 of Boeing Special Attention Service Bulletin 737-53-1234, Revision 1, dated... times specified in Figure 8 of Boeing Special Attention Service Bulletin 737-53-1234, Revision 1, dated... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE...

78 FR 60660 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-10-02

... compound (CIC) Boeing Material Specification (BMS) 3-23 to the surfaces of the repaired area. As an option... fracture and failure of the skin panels of the fuselage, and consequent rapid decompression of the airplane... availability of this material at the FAA, call 425-227-1221. Examining the AD Docket You may examine the AD...

BOES: Building Occupancy Estimation System using sparse ambient vibration monitoring

Science.gov (United States)

Pan, Shijia; Bonde, Amelie; Jing, Jie; Zhang, Lin; Zhang, Pei; Noh, Hae Young

2014-04-01

In this paper, we present a room-level building occupancy estimation system (BOES) utilizing low-resolution vibration sensors that are sparsely distributed. Many ubiquitous computing and building maintenance systems require fine-grained occupancy knowledge to enable occupant centric services and optimize space and energy utilization. The sensing infrastructure support for current occupancy estimation systems often requires multiple intrusive sensors per room, resulting in systems that are both costly to deploy and difficult to maintain. To address these shortcomings, we developed BOES. BOES utilizes sparse vibration sensors to track occupancy levels and activities. Our system has three major components. 1) It extracts features that distinguish occupant activities from noise prone ambient vibrations and detects human footsteps. 2) Using a sequence of footsteps, the system localizes and tracks individuals by observing changes in the sequences. It uses this tracking information to identify when an occupant leaves or enters a room. 3) The entering and leaving room information are combined with detected individual location information to update the room-level occupancy state of the building. Through validation experiments in two different buildings, our system was able to achieve 99.55% accuracy for event detection, less than three feet average error for localization, and 85% accuracy in occupancy counting.

78 FR 70851 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-11-27

... (EICAS) message that displays ``ENG THRUST (L,R)'' (for Model 787-8 airplanes) or ``ENG (1, 2, 3, or 4... The Boeing Company Model 747-8 and 747-8F series airplanes and Model 787-8 airplanes powered by GEnx...: 202-493-2251. Mail: U.S. Department of Transportation, Docket Operations, M-30, West Building Ground...

78 FR 73744 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-12-09

... paragraph (g) of this second SNPRM. Costs of Compliance We estimate that this proposed AD affects 1,147... 2,975 3,412,325 = $1,445. We have received no definitive data that would enable us to provide a cost... to be a rotable part, we note that Boeing Document D042A525, ``Compliance Document and Repair...

75 FR 68688 - Airworthiness Directives; The Boeing Company Model 757 Airplanes

Science.gov (United States)

2010-11-09

... the part number of a washer used in Figures 1 and 4 of Boeing Special Attention Service Bulletin 757... Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65, Seattle... Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE...

76 FR 8615 - Airworthiness Directives; The Boeing Company Model 767 Airplanes

Science.gov (United States)

2011-02-15

... distinct checks as specified in Steps 1 and 2 of Figures 2 and 5 of Boeing Special Attention Service..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65..., Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE...

Reductions in biomarkers of exposure (BoE) to harmful or potentially harmful constituents (HPHCs) following partial or complete substitution of cigarettes with electronic cigarettes in adult smokers.

Science.gov (United States)

O'Connell, Grant; Graff, Donald W; D'Ruiz, Carl D

2016-07-01

Changes in fifteen urine, blood and exhaled breath BoEs of HPHCs representing classes of compounds reported by FDA to be significant contributors to smoking-associated disease risks were measured in 105 clinical-confined subjects following randomization and a five-day forced-switch from usual brand conventional combustible cigarettes to: (i) exclusive commercial e-cigarette use; (ii) dual-use of commercial e-cigarettes and the subject's usual cigarette brand; or (iii) discontinued use of all tobacco or nicotine products. Levels of urinary biomarkers in subjects that completely substituted their usual cigarette with e-cigarettes were significantly lower (29-95%) after 5 days. Percent reductions in eight of nine urinary BoEs were indistinguishable to smokers who had quit smoking, except for nicotine equivalents, which declined by 25-40%. Dual users who halved self-reported daily cigarette consumption with e-cigarettes exhibited reductions (7-38%) in eight of nine urinary biomarkers, but had increase (1-20%) in nicotine equivalents. Reductions were broadly proportional to the reduced numbers of cigarettes smoked. Dual user urinary nicotine equivalents were slightly higher, but not statistically significant. After 5 days, blood nicotine biomarker levels were lower in the cessation (75-96%) and exclusive use groups (11-83%); with dual users experiencing no significant reductions. All subjects experienced significant decreases in exhaled CO. Decreases in the cessation and exclusive groups ranged from 88-89% and 27-32% in dual users. Exhaled NO increased in the cessation and exclusive groups (46-63% respectively), whereas the dual users experienced minimal changes. Overall, smokers who completely or partially substituted conventional cigarettes with e-cigarettes over five days, experienced reductions in HPHCs.

76 FR 42607 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -400ER Series Airplanes

Science.gov (United States)

2011-07-19

... CONTACT: Stephen Styskal, Aerospace Engineer, Cabin Safety and Environmental Systems Branch, ANM-150S, FAA...) 917-6439; fax: (425) 917-6590; e- mail: stephen[email protected] . SUPPLEMENTARY INFORMATION: Comments... Information (h) Actions done before the effective date of this AD in accordance with Boeing Special Attention...

Reductions in biomarkers of exposure (BoE) to harmful or potentially harmful constituents (HPHCs) following partial or complete substitution of cigarettes with electronic cigarettes in adult smokers

Science.gov (United States)

O’Connell, Grant; Graff, Donald W.; D’Ruiz, Carl D.

2016-01-01

Abstract Changes in fifteen urine, blood and exhaled breath BoEs of HPHCs representing classes of compounds reported by FDA to be significant contributors to smoking-associated disease risks were measured in 105 clinical-confined subjects following randomization and a five-day forced-switch from usual brand conventional combustible cigarettes to: (i) exclusive commercial e-cigarette use; (ii) dual-use of commercial e-cigarettes and the subject’s usual cigarette brand; or (iii) discontinued use of all tobacco or nicotine products. Levels of urinary biomarkers in subjects that completely substituted their usual cigarette with e-cigarettes were significantly lower (29–95%) after 5 days. Percent reductions in eight of nine urinary BoEs were indistinguishable to smokers who had quit smoking, except for nicotine equivalents, which declined by 25–40%. Dual users who halved self-reported daily cigarette consumption with e-cigarettes exhibited reductions (7–38%) in eight of nine urinary biomarkers, but had increase (1–20%) in nicotine equivalents. Reductions were broadly proportional to the reduced numbers of cigarettes smoked. Dual user urinary nicotine equivalents were slightly higher, but not statistically significant. After 5 days, blood nicotine biomarker levels were lower in the cessation (75–96%) and exclusive use groups (11–83%); with dual users experiencing no significant reductions. All subjects experienced significant decreases in exhaled CO. Decreases in the cessation and exclusive groups ranged from 88–89% and 27–32% in dual users. Exhaled NO increased in the cessation and exclusive groups (46–63% respectively), whereas the dual users experienced minimal changes. Overall, smokers who completely or partially substituted conventional cigarettes with e-cigarettes over five days, experienced reductions in HPHCs. PMID:27401591

Development of a self-navigating mobile interior robot application as a security guard/sentry

International Nuclear Information System (INIS)

Klarer, P.R.; Harrington, J.J.

1986-07-01

This paper describes a mobile robot system designed to function as part of an overall security system at a high security facility. The features of this robot system include specialized software and sensors for navigation without the need for external locator beacons or signposts, sensors for remote imaging and intruder detection, and the ability to communicate information either directly to the electronic portion of the security system or to a manned central control center. Other desirable features of the robot system include low weight, compact size, and low power consumption. The robot system can be operated either by remote manual control, or it can operate autonomously where direct human control can be limited to the global command level. The robot can act as a mobile remote sensing platform for alarm assessment or roving patrol, as a point sensor (sentry) in routine security applications, or as an exploratory device in situations potentially hazardous to humans. This robot system may also be used to ''walk-test'' intrusion detection sensors as part of a routine test and maintenance program for an interior intrusion detection system. The hardware, software, and operation of this robot system will be briefly described herein

ATM Technology Demonstration-1 Phase II Boeing Configurable Graphical Display (CGD) Software Design Description

Science.gov (United States)

Wilber, George F.

2017-01-01

This Software Description Document (SDD) captures the design for developing the Flight Interval Management (FIM) system Configurable Graphics Display (CGD) software. Specifically this SDD describes aspects of the Boeing CGD software and the surrounding context and interfaces. It does not describe the Honeywell components of the CGD system. The SDD provides the system overview, architectural design, and detailed design with all the necessary information to implement the Boeing components of the CGD software and integrate them into the CGD subsystem within the larger FIM system. Overall system and CGD system-level requirements are derived from the CGD SRS (in turn derived from the Boeing System Requirements Design Document (SRDD)). Display and look-and-feel requirements are derived from Human Machine Interface (HMI) design documents and working group recommendations. This Boeing CGD SDD is required to support the upcoming Critical Design Review (CDR).

Salapärane Boeing tõi Eestisse konteinereid / Ester Vilgats

Index Scriptorium Estoniae

Vilgats, Ester

2005-01-01

Ilmunud ka: Pärnu Postimees 8. nov., lk. 3, Postimees : na russkom jazõke 10. nov. lk. 10. Pärnu lennujaama juhataja Erki Teemägi ja Kaitsepolitsei Pärnu osakonna politseidirektor Kalev Kont kinnitasid, et Pärnu lennuväljal 12. jaanuaril 2003. aastal maandunud Boeing 737 vedas kaubakonteinereid, mitte terroristidest sõjavange, nagu väitis ajaleht Washington Post. Vt. samas: Neeme Raud. Inimõiguslased ootavad euroliidu sekkumist

A review of acceptance testing of the Los Alamos, Canberra Alpha Sentry Continuous Air Monitor (CAM)

International Nuclear Information System (INIS)

Rodgers, J.C.

1998-01-01

Los Alamos National Laboratory (LANL) undertook the design and development of a new generation of alpha continuous air monitor (CAM) instrumentation that would incorporate advanced technologies in the design of the sampling inlet, multi-channel analyzer (MCA) electronics, solid state alpha detectors, radon background interference suppression, background interference compensation and based on spectral analysis, and microcomputer based data communication, processing, storage, and retrieval. The ANSI air monitoring instrument standards (Performance Specifications for Health Physics Instrumentation -- Occupational Airborne Radioactivity Monitoring Instrumentation, N42.17B) specify performance criteria and testing procedures for instruments and instrument systems designed to continuously sample and quantify airborne radioactivity in the workplace. Although the intent of the standard is to provide performance testing criteria for type testing, it is appropriate to evaluate the performance of a new instrument such as the Alpha Sentry against certain of these criteria for purposes of an acceptance test based on stated specifications and the Los Alamos CAM Requirements document. This report provides an overview of the results of these tests, as they pertain to instruments designed to detect alpha-emitting radionuclides in particulate form

78 FR 43839 - Airworthiness Directives; the Boeing Company

Science.gov (United States)

2013-07-22

.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New... AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P. O. Box 3707, MC 2H..., performed in accordance with Part 4 and Figure 8 of this service bulletin. This service information is being...

Final Technical Report. Project Boeing SGS

Energy Technology Data Exchange (ETDEWEB)

Bell, Thomas E. [The Boeing Company, Seattle, WA (United States)

2014-12-31

Boeing and its partner, PJM Interconnection, teamed to bring advanced “defense-grade” technologies for cyber security to the US regional power grid through demonstration in PJM’s energy management environment. Under this cooperative project with the Department of Energy, Boeing and PJM have developed and demonstrated a host of technologies specifically tailored to the needs of PJM and the electric sector as a whole. The team has demonstrated to the energy industry a combination of processes, techniques and technologies that have been successfully implemented in the commercial, defense, and intelligence communities to identify, mitigate and continuously monitor the cyber security of critical systems. Guided by the results of a Cyber Security Risk-Based Assessment completed in Phase I, the Boeing-PJM team has completed multiple iterations through the Phase II Development and Phase III Deployment phases. Multiple cyber security solutions have been completed across a variety of controls including: Application Security, Enhanced Malware Detection, Security Incident and Event Management (SIEM) Optimization, Continuous Vulnerability Monitoring, SCADA Monitoring/Intrusion Detection, Operational Resiliency, Cyber Range simulations and hands on cyber security personnel training. All of the developed and demonstrated solutions are suitable for replication across the electric sector and/or the energy sector as a whole. Benefits identified include; Improved malware and intrusion detection capability on critical SCADA networks including behavioral-based alerts resulting in improved zero-day threat protection; Improved Security Incident and Event Management system resulting in better threat visibility, thus increasing the likelihood of detecting a serious event; Improved malware detection and zero-day threat response capability; Improved ability to systematically evaluate and secure in house and vendor sourced software applications; Improved ability to continuously monitor

78 FR 14644 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-03-07

... support clamps installed within the left environmental control systems (ECS) bay, which could allow wiring... the installation of winglets per Supplemental Type Certificate (STC) ST00830SE ( http://rgl.faa.gov... Change the ``Parts Installation Prohibition'' Section Boeing requested the ``Parts Installation...

77 FR 38547 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-06-28

... (including cracking, misdrilled fastener holes, elongated fastener holes, corrosion, oil-cans, and existing..., corrosion, oil-cans, and existing repairs), or for certain discrepancies, contacting Boeing for repair... Designated Engineering Representative (DER). We have revised the date of the document specified in paragraph...

77 FR 5195 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-02-02

...). Support for Proposed Actions United Parcel Service Co. (UPS) supports mandating the Stage 2 inspections...., Washington, DC 20590, between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. For service information identified in this proposed AD, contact Boeing Commercial Airplanes, Attention: Data & Services...

77 FR 49708 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-08-17

.... ADDRESSES: For service information identified in this AD, contact Boeing Commercial Airplanes, Attention.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New... Figure 24 as an accepted procedure'' for the HFEC inspection, this AD requires that the inspection be...

77 FR 47267 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-08-08

... contained within those paragraphs. Request To Clarify Undefined Requirement UPS stated that the actions... modification, which is currently undefined in Boeing Service Bulletin 747 53A2559, Revision 1, dated August 4... believe it will be affected; however, UPS is concerned with the precedent of mandating undefined...

78 FR 26286 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-05-06

... the first post-modification high frequency eddy current (HFEC) repetitive inspection may be extended... Boeing (the original equipment manufacturer) had time to build up an adequate stock of kits and/or frames.... We have also revised paragraph (g) in this supplemental NPRM to correspond to the manufacturer's...

78 FR 31389 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-05-24

... ``New Inspections and Corrective Actions to Ensure Cross Beam Reinforcement,'' and suggested language... door. Boeing added that the current language in the NPRM and this service information leave open the...) of this AD. (i) For airplanes with line numbers 1 through 1231 inclusive: Install a cross beam repair...

78 FR 68693 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-11-15

... Special Attention Service Bulletin 737-53-1296, dated January 11, 2011, in Step 1, ``Move,'' of Figure 10.... ADDRESSES: For service information identified in this AD, contact Boeing Commercial Airplanes, Attention... Facility, U.S. Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12...

77 FR 60285 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-10-03

... the MLG for chicken- wire cracks, corrosion, and chrome plate distress; repetitive magnetic particle..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65... magnetic particle inspections of the outer diameter chrome on the center axles of the MLG for cracks; and...

77 FR 61548 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-10-10

...-indicating and crew-alerting system (EICAS) messages ALT DISAGREE and IAS DISAGREE displayed. The flightcrew... Special Attention Service Bulletin 727-34-0245, dated June 4, 2008 (for Model airplanes); [[Page 61549... -200C series airplanes); and Boeing Special Attention Service Bulletin 747-34-2925, dated June 4, 2008...

77 FR 63714 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-10-17

.... ACTION: Final rule. SUMMARY: We are adopting a new airworthiness directive (AD) for certain The Boeing... instructions and doing the work specified in those instructions. We are issuing this AD to detect and correct..., Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590. FOR FURTHER INFORMATION CONTACT: Kelly...

78 FR 57053 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-09-17

... Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65, Seattle, WA... Management Facility, U.S. Department of Transportation, Docket Operations, M-30, West Building Ground Floor... frame from stringers 23 through 31 per Figure 5 or Figure 6 of the service bulletins specified in...

77 FR 52212 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-08-29

... request to revise this AD. The inspections specified in Figure 1 in both Boeing Special Attention Service... Commercial Airplanes, Attention: Data & Services Management, P. O. Box 3707, MC 2H-65, Seattle, Washington... Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590. FOR FURTHER...

78 FR 14469 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-03-06

...-2251. Mail: U.S. Department of Transportation, Docket Operations, M-30, West Building Ground Floor... identified in this proposed AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P... through 31 per Figure 5 or Figure 6 of the service bulletins specified in paragraph (g) or (h) of this AD...

77 FR 54854 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-09-06

.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New... 5 p.m., Monday through Friday, except Federal holidays. Boeing Commercial Airplanes, Attention: Data... airplanes), except as specified in paragraphs (h)(1) and (h)(2) of this AD. (1) Steps 1 through 5 of Figure...

77 FR 16188 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-03-20

..., M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590... Airplanes, Attention: Data & Services Management, P. O. Box 3707, MC 2H-65, Seattle, Washington 98124-2207... AD would require repair using a method approved in accordance with paragraph (l). Figure 1 of Boeing...

76 FR 63163 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

Science.gov (United States)

2011-10-12

..., January 18, 2011) as follows: ``Figure 1 of the Accomplishment Instructions of Boeing Special Attention... Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65, Seattle... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590...

78 FR 61161 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-10-03

... bolts being fabricated from bar stock with a machined head instead of from a forged blank with an upset... are issuing this AD to prevent damage to the MLG and consequent damage to airplane structure, which...'' fabrication. Boeing stated that it approved the fabrication of the bolts from bar stock with a machined head...

77 FR 64711 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2012-10-23

... Service Bulletin 737-22A1224, dated May 18, 2012, requires installation of FCC software P/N 2274-COL-AC1..., 2010, because that service bulletin only specifies to install FCC OPS software P/N 2276-COL-AC1-05 or P...) Install software identified in table 2 of the Accomplishment Instructions of Boeing Alert Service Bulletin...

78 FR 22439 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2013-04-16

..., West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590. Hand..., Attention: Data & Services Management, P. O. Box 3707, MC 2H-65, Seattle, WA 98124-2207; phone: 206-544-5000... SRM 53-30-1, Figures 20, 21, 31, or 32; and Boeing 737-300/-400/-500 SRM 53-10-01, Repair 5, 6, or 8...

77 FR 69747 - Airworthiness Directives; The Boeing Company Model Airplanes

Science.gov (United States)

2012-11-21

... cycles after the effective date of this AD.'' The new language in paragraph (i)(1) of the NPRM states... language specified in paragraphs (l) through (n) of the NPRM from paragraph (o) of the NPRM. In the... inclusive. Boeing stated that the NPRM requirements do not differentiate for Model 737-200 series airplanes...

Human Systems Integration Requirements

Science.gov (United States)

2009-09-01

52 Stratofortress, C/ KC -135 Stratotanker, E-3 Sentry and contractor logistics support aircraft; as well as a substantial jet engine inventory...www.hqda.army.mil/ teo /Sutability%20Terms%20MOA%20Oct%2005.pdf q. ISO 9241-11, Ergonomic requirements for office work with visual display terminals (VDTs

Effect of BioSentry 904 and ethylenediaminetetraacetic acid-tris disinfecting during incubation of chicken eggs on microbial levels and productivity of poultry.

Science.gov (United States)

Walker, S E; Sander, J E

2004-01-01

Proper sanitation practices and the use of efficacious disinfectants in a hatchery have an effect on chick quality. Aerosol bacterial counts, egg moisture loss, hatchability, chick quality, and broiler productivity were evaluated when egg surfaces were contaminated by immersion of each egg into a broth medium containing a field isolate of Pseudomonas aeruginosa and incubated with exposure to one of three disinfectant treatments administered by fine spray: distilled water, BioSentry 904 (904), and a 1:1 ratio of 904 and ethylenediaminetetraacetic acid (EDTA)-Tris. The aerosol bacteria levels were statistically greater on day 21 of incubation in the group treated with distilled water than in those receiving disinfectants. Overall hatch of fertile eggs and egg moisture loss were comparable among all three treatments. At 1 day of age, the chicks incubated with 904 had a statistically lower yolk sac contamination rate than those incubated with 904+EDTA-Tris or distilled water. The 2-wk mortality rates, body weights, feed conversion ratios, yolk sac weights, and yolk sac contamination rates were all similar among the three treatments.

75 FR 9760 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747...

Science.gov (United States)

2010-03-04

.... ADDRESSES: For service information identified in this AD, contact Boeing Commercial Airplanes, Attention... Management Facility, U.S. Department of Transportation, Docket Operations, M-30, West Building Ground Floor.... Figure 31, Sheet 7, of the Accomplishment Instructions of Boeing Alert Service Bulletin 747- 51A2060...

Influence of Machine-Derived Smoke Yields on Biomarker of Exposure (BOE Levels in Cigarette Smokers*

Directory of Open Access Journals (Sweden)

Scherer Gerhard

2015-01-01

Full Text Available Individual uptake of tobacco smoke constituents by smoking is highly variable in cigarette smokers and cannot be predicted by smoking behaviour variables and machine-derived smoke yields. It is well established that uptake of smoke constituents is best described by a series of bio-markers of exposure (BOEs such as metabolites of nico-tine, tobacco-specific nitrosamines (TSNAs, polycyclic aromatic hydrocarbons (PAHs, aromatic amines, benzene, 1,3-butadiene, acrolein, hydrogen cyanide, 2,5-dimethyl-furan and other smoke constituents.

75 FR 61999 - Airworthiness Directives; The Boeing Company Model 767 Airplanes

Science.gov (United States)

2010-10-07

... new rework procedures, is necessary. We also have reviewed Boeing Service Bulletins 767-57A0094 (for... Service Bulletin 767-57-0068, Revision 1, dated May 9, 2002, specifies that the compliance time to rework...,236 $6,256 255 $1,595,280. (required by AD 2001-14-19). Bonding jumper assembly rework 4 322 $662 47...

Data Mining of NASA Boeing 737 Flight Data: Frequency Analysis of In-Flight Recorded Data

Science.gov (United States)

Butterfield, Ansel J.

2001-01-01

Data recorded during flights of the NASA Trailblazer Boeing 737 have been analyzed to ascertain the presence of aircraft structural responses from various excitations such as the engine, aerodynamic effects, wind gusts, and control system operations. The NASA Trailblazer Boeing 737 was chosen as a focus of the study because of a large quantity of its flight data records. The goal of this study was to determine if any aircraft structural characteristics could be identified from flight data collected for measuring non-structural phenomena. A number of such data were examined for spatial and frequency correlation as a means of discovering hidden knowledge of the dynamic behavior of the aircraft. Data recorded from on-board dynamic sensors over a range of flight conditions showed consistently appearing frequencies. Those frequencies were attributed to aircraft structural vibrations.

76 FR 22828 - Airworthiness Directives; The Boeing Company Model 737-700 Series Airplanes

Science.gov (United States)

2011-04-25

... January 20, 2010. Those special attention service bulletins describe procedures for doing a general visual... General Visual Inspection of Seat Tracks (i) For airplanes identified in Boeing Special Attention Service... results from reports that the aft seat leg fittings span the station (STA) 521.45 stay-out zone. We are...

Advanced composite elevator for Boeing 727 aircraft

Science.gov (United States)

1979-01-01

Detail design activities are reported for a program to develop an advanced composites elevator for the Boeing 727 commercial transport. Design activities include discussion of the full scale ground test and flight test activities, the ancillary test programs, sustaining efforts, weight status, and the production status. Prior to flight testing of the advanced composites elevator, ground, flight flutter, and stability and control test plans were reviewed and approved by the FAA. Both the ground test and the flight test were conducted according to the approved plan, and were witnessed by the FAA. Three and one half shipsets have now been fabricated without any significant difficulty being encountered. Two elevator system shipsets were weighed, and results validated the 26% predicted weight reduction. The program is on schedule.

Simulation model for the Boeing 720B aircraft-flight control system in continuous flight.

Science.gov (United States)

1971-08-01

A mathematical model of the Boeing 720B aircraft and autopilot has been derived. The model is representative of the 720B aircraft for continuous flight within a flight envelope defined by a Mach number of .4 at 20,000 feet altitude in a cruise config...

Advanced composite elevator for Boeing 727 aircraft. Volume 1: Technical summary

Science.gov (United States)

Chovil, D. V.; Harvey, S. T.; Mccarty, J. E.; Desper, O. E.; Jamison, E. S.; Syder, H.

1981-01-01

The design, development, analysis, and testing activities and results that were required to produce five and one-half shipsets of advanced composite elevators for Boeing 727 aircraft are summarized. During the preliminary design period, alternative concepts were developed. After selection of the best design, detail design and basic configuration improvements were evaluated. Five and one-half shipsets were manufactured. All program goals (except competitive cost demonstration) were accomplished when our design met or exceeded all requirements, criteria, and objectives.

75 FR 22514 - Airworthiness Directives; The Boeing Company Model 747-200B Series Airplanes

Science.gov (United States)

2010-04-29

... Administration (FAA), DOT. ACTION: Final rule; request for comments. SUMMARY: We are adopting a new airworthiness... cracking of the fuselage skin lap joints at stringer 6 on the left and right sides from station (STA) 340... repetitive inspections for the modified area. This AD results from reviews done by Boeing, which show that...

76 FR 39763 - Special Conditions: Boeing Model 787-8 Airplane; Interaction of Systems and Structures...

Science.gov (United States)

2011-07-07

... Conditions No. 25-354A-SC] Special Conditions: Boeing Model 787-8 Airplane; Interaction of Systems and..., electronic flight control system control surface awareness, HIRF protection, limit engine torque loads for... standards. A special condition is needed to require consideration of the effects of systems on the...

Antifungal susceptibilities of Candida, Cryptococcus neoformans and Aspergillus fumigatus from the Asia and Western Pacific region: data from the SENTRY antifungal surveillance program (2010-2012).

Science.gov (United States)

Pfaller, Michael A; Messer, Shawn A; Jones, Ronald N; Castanheira, Mariana

2015-09-01

The SENTRY Antifungal Surveillance Program monitors global susceptibility rates of newer and established antifungal agents. We report the in vitro activity of seven antifungal agents against 496 contemporary clinical isolates of yeasts and molds. The isolates were obtained from 20 laboratories in the Asia-Western Pacific (APAC) region during 2010 through 2012. Anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole and voriconazole were susceptibility tested using CLSI methods and species-specific interpretive criteria. Sequencing of fks hot spots was performed for echinocandin-resistant strains. Isolates included 13 species of Candida (n=460), 5 species of non-Candida yeasts (21), 5 species of Aspergillus (11) and 4 other molds. Echinocandin resistance was uncommon among eight species of Candida and was only detected in three isolates of Candida glabrata, two from Australia harboring mutations in fks1 (F625S) and fks2 (S663P). Resistance to the azoles was much more common and was observed among all species with the exception of Candida dubliniensis. Fluconazole resistance rates observed with C. glabrata (6.8%) was comparable to that seen with Candida parapsilosis (5.7%) and Candida tropicalis (3.6%). Cross resistance among the triazoles was seen with each of these three species. The mold-active azoles and the echinocandins were all active against isolates of Aspergillus fumigatus. Azole resistance was not detected among the isolates of Cryptococcus neoformans. Antifungal resistance is uncommon among isolates of fungi causing invasive fungal infections in the APAC region. As in other regions of the world, emerging resistance to the echinocandins among invasive isolates of C. glabrata bears close monitoring.

Schmidt Reaction of E-3-Benzylidenechromanones and E-3-Benzylidenethiochromanones

Directory of Open Access Journals (Sweden)

Tapas K. Mandal

2013-01-01

Full Text Available On treatment with NaN3/c. H2SO4-HOAc or NaN3/TFA, E-3-benzylidenechromanones are mostly converted to E-β-aminobenzylidenechromanones while E-3-benzylidenethiochromanones are converted to 3-benzoylthiochromones. A structurally new type of product has been isolated for the reaction of E-3-benzylidene-4′-methoxychromanone with NaN3/TFA. Mechanistic paths have been suggested for formation of the products.

STS-65 crewmembers participate in bench review at Boeing Building

Science.gov (United States)

1994-01-01

Attired in clean suits, STS-65 Payload Commander (PLC) Richard J. Hieb (left) and Payload Specialist Chiaki Mukai examine the contents of a stowage locker during a bench review at Boeing's Flight Equipment Processing Facility (FEPF) near the Johnson Space Center (JSC). In the background, Commander Robert D. Cabana inspects additional equipment to be carried aboard the Space Shuttle Columbia, Orbiter Vehicle (OV) 102, for the scheduled July flight of the second International Microgravity Laboratory (IML-2) mission. Mukai represents Japan's National Space Development Agency (NASDA). Photo taken by NASA JSC contract photographer Scott A. Wickes.

Full-scale testing, production and cost analysis data for the advanced composite stabilizer for Boeing 737 aircraft, volume 2

Science.gov (United States)

Aniversario, R. B.; Harvey, S. T.; Mccarty, J. E.; Parson, J. T.; Peterson, D. C.; Pritchett, L. D.; Wilson, D. R.; Wogulis, E. R.

1982-01-01

The development, testing, production activities, and associated costs that were required to produce five-and-one-half advanced-composite stabilizer shipsets for Boeing 737 aircraft are defined and discussed.

75 FR 2433 - Special Conditions: Boeing Model 747-8/-8F Airplanes, Systems and Data Networks Security...

Science.gov (United States)

2010-01-15

... Conditions No. 25-401-SC] Special Conditions: Boeing Model 747-8/-8F Airplanes, Systems and Data Networks Security--Protection of Airplane Systems and Data Networks From Unauthorized External Access AGENCY... that effective electronic system security protection strategies are implemented to protect the airplane...

Quantitative Analysis for Installation Access Planning at Naval Base San Diego

Science.gov (United States)

2012-09-01

VPH in one processing lane, then we can assume that if the SECO were to open another sentry processing lane, the total throughput of both lanes would...be 600 VPH . Similarly, if two sentries in tandem can produce a throughput of 500 VPH , then having two lanes with two sentries in tandem each will...produce a total throughput of 1000 VPH . We assume throughout that there is no server idleness and so there is essentially an infinite backlog of

76 FR 24354 - Airworthiness Directives; The Boeing Company Model 777-200 and -300 Series Airplanes Equipped...

Science.gov (United States)

2011-05-02

... this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New.... Required parts cost about $122,617 per product. Based on these figures, we estimate the cost of this AD to...

75 FR 9137 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

Science.gov (United States)

2010-03-01

... AD, contact Boeing Commercial Airplanes, P.O. Box 3707, Seattle, Washington 98124-2207. Examining the... repetitive detailed and magnetic particle inspections to detect discrepancies (including corrosion, pitting... detailed inspection for corrosion, pitting, and cracking of the carriage spindle, the magnetic particle...

78 FR 68986 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

Science.gov (United States)

2013-11-18

.... SUPPLEMENTARY INFORMATION: Background On August 21, 2012, The Boeing Company applied for a change to Type... vulnerabilities from intentional or unintentional corruption of data and systems critical to the safety and maintenance of the airplane. The existing regulations and guidance material did not anticipate this type of...

78 FR 68985 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

Science.gov (United States)

2013-11-18

... Boeing Company applied for a change to Type Certificate No. T00001SE Rev. 30 dated June 6, 2012 for... integration may result in security vulnerabilities from intentional or unintentional corruption of data and... material did not anticipate this type of system architecture or electronic access to aircraft systems...

75 FR 5677 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

Science.gov (United States)

2010-02-04

... AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H... a magnetic particle inspection (MPI) in accordance with Part 4, ``Magnetic Particle Inspection of... November 5, 2009, or MPI in accordance with Part 4, ``Magnetic Particle Inspection of the Midspar Fuse Pin...

The DoD C-17 Versus the Boeing 777. A Comparison of Acquisition and Development

Science.gov (United States)

1999-10-01

Standard Encyclopedia (New York: Unicorn Publishers, 1955), 102-103. 9. The World Book Encyclopedia (Kansas City, MO: Field Enterprises Educational...costs about $25.25 per mile to operate--including insurance, administration, and financing . The amount is similar for the MD-11 and Airbus A340-300X, 9...resources that include investments, income from operations (airplane sales), or outside financing . Although Boeing customarily receives a deposit

76 FR 60710 - Airworthiness Directives; The Boeing Company Model 737-600, -700, -700C, -800, and -900 Series...

Science.gov (United States)

2011-09-30

... universal fault interrupters (UFIs) using a certain supplemental type certificate. This new AD continues to... interrupter (GFI) feature, or installation and maintenance of universal fault interrupters (UFIs) using a... section of this AD to include this contact information. Request To Revise a Word Boeing requested a change...

75 FR 81508 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

Science.gov (United States)

2010-12-28

... require installing new panels in the main equipment center, making certain wiring changes, installing new..., Boeing found indications of wiring deterioration that could cause electrical faults in the main tank... center; making certain wiring changes; installing new GFI relays in the P301 and P302 panels; and...

Boeing's STAR-FODB test results

Science.gov (United States)

Fritz, Martin E.; de la Chapelle, Michael; Van Ausdal, Arthur W.

1995-05-01

Boeing has successfully concluded a 2 1/2 year, two phase developmental contract for the STAR-Fiber Optic Data Bus (FODB) that is intended for future space-based applications. The first phase included system analysis, trade studies, behavior modeling, and architecture and protocal selection. During this phase we selected AS4074 Linear Token Passing Bus (LTPB) protocol operating at 200 Mbps, along with the passive, star-coupled fiber media. The second phase involved design, build, integration, and performance and environmental test of brassboard hardware. The resulting brassboard hardware successfully passed performance testing, providing 200 Mbps operation with a 32 X 32 star-coupled medium. This hardware is suitable for a spaceflight experiment to validate ground testing and analysis and to demonstrate performace in the intended environment. The fiber bus interface unit (FBIU) is a multichip module containing transceiver, protocol, and data formatting chips, buffer memory, and a station management controller. The FBIU has been designed for low power, high reliability, and radiation tolerance. Nine FBIUs were built and integrated with the fiber optic physical layer consisting of the fiber cable plant (FCP) and star coupler assembly (SCA). Performance and environmental testing, including radiation exposure, was performed on selected FBIUs and the physical layer. The integrated system was demonstrated with a full motion color video image transfer across the bus while simultaneously performing utility functions with a fiber bus control module (FBCM) over a telemetry and control (T&C) bus, in this case AS1773.

75 FR 8554 - Airworthiness Directives; The Boeing Company Model 747-100, -200B, and -200F Series Airplanes

Science.gov (United States)

2010-02-25

...-0132; Directorate Identifier 2009-NM-096-AD] RIN 2120-AA64 Airworthiness Directives; The Boeing Company... approaching or have exceeded their design life goal. We issued that AD to prevent separation of fuselage skin...,000 flight cycles to 1,000 flight cycles. Differences Between the Proposed AD and Service Bulletin...

Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex.

Science.gov (United States)

Aylett, Christopher H S; Boehringer, Daniel; Erzberger, Jan P; Schaefer, Tanja; Ban, Nenad

2015-03-01

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.

E3 Staff Database

Data.gov (United States)

US Agency for International Development — E3 Staff database is maintained by E3 PDMS (Professional Development & Management Services) office. The database is Mysql. It is manually updated by E3 staff as...

E3Net: a system for exploring E3-mediated regulatory networks of cellular functions.

Science.gov (United States)

Han, Youngwoong; Lee, Hodong; Park, Jong C; Yi, Gwan-Su

2012-04-01

Ubiquitin-protein ligase (E3) is a key enzyme targeting specific substrates in diverse cellular processes for ubiquitination and degradation. The existing findings of substrate specificity of E3 are, however, scattered over a number of resources, making it difficult to study them together with an integrative view. Here we present E3Net, a web-based system that provides a comprehensive collection of available E3-substrate specificities and a systematic framework for the analysis of E3-mediated regulatory networks of diverse cellular functions. Currently, E3Net contains 2201 E3s and 4896 substrates in 427 organisms and 1671 E3-substrate specific relations between 493 E3s and 1277 substrates in 42 organisms, extracted mainly from MEDLINE abstracts and UniProt comments with an automatic text mining method and additional manual inspection and partly from high throughput experiment data and public ubiquitination databases. The significant functions and pathways of the extracted E3-specific substrate groups were identified from a functional enrichment analysis with 12 functional category resources for molecular functions, protein families, protein complexes, pathways, cellular processes, cellular localization, and diseases. E3Net includes interactive analysis and navigation tools that make it possible to build an integrative view of E3-substrate networks and their correlated functions with graphical illustrations and summarized descriptions. As a result, E3Net provides a comprehensive resource of E3s, substrates, and their functional implications summarized from the regulatory network structures of E3-specific substrate groups and their correlated functions. This resource will facilitate further in-depth investigation of ubiquitination-dependent regulatory mechanisms. E3Net is freely available online at http://pnet.kaist.ac.kr/e3net.

75 FR 12657 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, -300ER, and 777F Series...

Science.gov (United States)

2010-03-17

..., Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590. Hand Delivery: U.S. Department of... Boeing Company has also discovered during flight simulations that the climb gradient is less than optimal... bulletin describes procedures for installing new operational program software for the left, center, and...

76 FR 24349 - Airworthiness Directives; The Boeing Company Model 747-200B, -300, -400, -400D, and -400F Series...

Science.gov (United States)

2011-05-02

... this AD, contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC.... Department of Transportation, Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New... to this issue. Request To Clarify Figures in Service Information UPS requested that we clarify the...

STS-65 crewmembers and backup participate in bench review at Boeing Building

Science.gov (United States)

1994-01-01

STS-65 crewmembers, wearing clean suits, look over equipment to be carried aboard the Space Shuttle Columbia, Orbiter Vehicle (OV) 102, for the scheduled July flight of the second International Microgravity Laboratory (IML-2) mission. Surrounding a table full of equipment at Boeing's Flight Equipment Processing Facility near the Johnson Space Center (JSC), are (left to right) Mission Specialist (MS) Leroy Chiao, MS Donald A. Thomas, Commander Robert D. Cabana, Payload Specialist Chiaki Mukai, Payload Commander (PLC) Richard J. Hieb, and backup (alternate) payload specialist Jean-Jacques Favier. Mukai represents Japan's National Space Development Agency (NASDA) and Favier represents France's Centre National d'Etudes Spatiales. Photo taken by NASA JSC contract photographer Scott A. Wickes.

Study of charge symmetry in 4He by simultaneous 4He(e,e'p)3H and 4He(e,e'n)3He measurements

International Nuclear Information System (INIS)

Spahn, M.; Kihm, T.; Knopfle, K.T.; Friedrich, J.; Voegler, N.; Schmitt, C.; Walther, V.H.; Unkelbach, M.; Hofmann, H.M.

1989-01-01

We have measured the ( 3 H+p) and ( 3 He+n) breakup of 4 He by 4 He(e,e'c) coincidence experiments at low momentum transfer (q∼0.39 fm -1 ) in the excitation energy region 22≤E x ≤36 MeV. Both channels were studied simultaneously by detecting the charged fragments c=p, 3 H, and 3 He in the same detector system. The measured 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He angular correlations as well as the deduced total 4 He(e,e'p) 3 H and 4 He(e,e'n) 3 He cross sections are reproduced by microscopic model calculations with a charge-symmetric nuclear Hamiltonian

75 FR 52907 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747...

Science.gov (United States)

2010-08-30

... CONTACT: Nicholas Han, Aerospace Engineer, Airframe Branch, ANM-120S, FAA, Seattle Aircraft Certification... Airlines (JAL) reports that certain structures prevented the accomplishment of the inspection specified in... time for certain inspection locations. Boeing reports that recent engineering analysis has revealed...

75 FR 10669 - Airworthiness Directives; The Boeing Company Model 747-100, 747-200B, 747-300, and 747SR Series...

Science.gov (United States)

2010-03-09

... base assembly to the moisture shroud as given in Figure 10 in Boeing Alert Service Bulletin 747- 25A3368, Revision 2, dated June 12, 2008, and to fill any unused pilot holes in the mounting base assembly... 25, 2007, and to fill any unused pilot holes in the mounting base assembly in accordance with the...

Full-scale testing, production and cost analysis data for the advanced composite stabilizer for Boeing 737 aircraft. Volume 1: Technical summary

Science.gov (United States)

Aniversario, R. B.; Harvey, S. T.; Mccarty, J. E.; Parsons, J. T.; Peterson, D. C.; Pritchett, L. D.; Wilson, D. R.; Wogulis, E. R.

1983-01-01

The full scale ground test, ground vibration test, and flight tests conducted to demonstrate a composite structure stabilizer for the Boeing 737 aircraft and obtain FAA certification are described. Detail tools, assembly tools, and overall production are discussed. Cost analyses aspects covered include production costs, composite material usage factors, and cost comparisons.

75 FR 61337 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747...

Science.gov (United States)

2010-10-05

..., contact Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65..., Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE...-60-15, Figure 201, Repair 5, do the inspection, including the threshold and intervals in accordance...

Acoustic Emission Methodology to Evaluate the Fracture Toughness in Heat Treated AISI D2 Tool Steel

Science.gov (United States)

Mostafavi, Sajad; Fotouhi, Mohamad; Motasemi, Abed; Ahmadi, Mehdi; Sindi, Cevat Teymuri

2012-10-01

In this article, fracture toughness behavior of tool steel was investigated using Acoustic Emission (AE) monitoring. Fracture toughness ( K IC) values of a specific tool steel was determined by applying various approaches based on conventional AE parameters, such as Acoustic Emission Cumulative Count (AECC), Acoustic Emission Energy Rate (AEER), and the combination of mechanical characteristics and AE information called sentry function. The critical fracture toughness values during crack propagation were achieved by means of relationship between the integral of the sentry function and cumulative fracture toughness (KICUM). Specimens were selected from AISI D2 cold-work tool steel and were heat treated at four different tempering conditions (300, 450, 525, and 575 °C). The results achieved through AE approaches were then compared with a methodology proposed by compact specimen testing according to ASTM standard E399. It was concluded that AE information was an efficient method to investigate fracture characteristics.

75 FR 27424 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747...

Science.gov (United States)

2010-05-17

... Boeing Commercial Airplanes, Attention: Data & Services Management, P.O. Box 3707, MC 2H-65, Seattle... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590... reviewed the figures we have used over the past several years to calculate AD costs to operators. To...

High-momentum components in 3He(e,e') and (e,e'p)

International Nuclear Information System (INIS)

Sick, I.

1984-01-01

In this talk, the author discusses the properties of the 3 He spectral function S(K,E) at large momentum K and large separation energy E. He deals in particular with the question how to measure S, and how to understand the physics connected to it. As experimental input he uses (e,e') data. He starts by discussing how to understand (e,e') in terms of properties of S(K,E). For this purpose, an extensive discussion of the scaling function F(y) is needed. When once it is found out how to exploit inclusive scattering, he comes back to exclusive reactions and discusses the relative merits of (e,e'p) and (e,e') in order to investigate how these tools can best complement each other. Some conclusions on the properties of S(K,E) at large K and E, together with some suggestions for future activities follows. (Auth.)

The electrodisintegration of 3He studied with the 3He(e,e'p)2H and 3He(e,e'd)1H reactions

International Nuclear Information System (INIS)

Keizer, P.H.M.

1986-01-01

The author presents a survey of experimental and theoretical results for the ground-state properties of 3 He. The formalism of the (e,e'p) and (e,e'd) reactions is presented. A short description of the instrumentation, in particular those elements which are typical for the present study, and of the data analysis is given. This includes a description of the elements required in the 'recoil detection' technique. The kinematics of the experiments and the results are presented. The results are discussed and compared with calculations. (Auth.)

Radiative detection of spin 3/2 particles in e+e- interactions

International Nuclear Information System (INIS)

Klimenko, K.G.; Tikhonin, F.F.

1983-01-01

Cross-section for the process e + e - → γgsub(3/2)gtidlesub(3/2) has been calculated all the spin projections being taken into account. Photon energy dependence differs greatly from that of process e + e - →γωω tidle. In the case of eXistence of spin 3/2 neutral fermions such processes give a good possibility for their signal detection

The cauliflower Orange gene enhances petiole elongation by suppressing expression of eukaryotic release factor 1.

Science.gov (United States)

Zhou, Xiangjun; Sun, Tian-Hu; Wang, Ning; Ling, Hong-Qing; Lu, Shan; Li, Li

2011-04-01

The cauliflower (Brassica oleracea var. botrytis) Orange (Or) gene affects plant growth and development in addition to conferring β-carotene accumulation. This study was undertaken to investigate the molecular basis for the effects of the Or gene mutation in on plant growth. The OR protein was found to interact with cauliflower and Arabidopsis eukaryotic release factor 1-2 (eRF1-2), a member of the eRF1 family, by yeast two-hybrid analysis and by bimolecular fluorescence complementation (BiFC) assay. Concomitantly, the Or mutant showed reduced expression of the BoeRF1 family genes. Transgenic cauliflower plants with suppressed expression of BoeRF1-2 and BoeRF1-3 were generated by RNA interference. Like the Or mutant, the BoeRF1 RNAi lines showed increased elongation of the leaf petiole. This long-petiole phenotype was largely caused by enhanced cell elongation, which resulted from increased cell length and elevated expression of genes involved in cell-wall loosening. These findings demonstrate that the cauliflower Or gene controls petiole elongation by suppressing the expression of eRF1 genes, and provide new insights into the molecular mechanism of leaf petiole regulation. © 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.

NASA N3-X with Turboelectric Distributed Propulsion

Science.gov (United States)

Felder, James L.

2014-01-01

Presentation summarizing the phase I study of the NASA N3-X turboelectric distributed propulsion power aircraft to the IMechE Disruptive Green Propulsion Technologies conference in London, UK November 16th and 17th, 2014. This presentation contains the results of a NASA internal study funded by the NASA Fixed Wing program to look at the application of turboelectric distributed propulsion to a long-range 300 seat aircraft. The reference aircraft is the Boeing 777-200LR. The N3-X reduced energy consumption by 70 compared to the 777-200LR, LTO NOx by 85 compared to the CAEP 6 limits, and noise by 32-64 EPNdB depending on engine placement compared to the stage 4 noise standards. This exceeded the N+3 metrics of reducing energy by 60, LTO NOx by 80, and noise by 52 EPNdB. Cruise NOx was not estimated, but likely meet the 80 reduction goal as well.

(e,3e) collisions on He in the quasi-dipolar regime

International Nuclear Information System (INIS)

Taouil, I.; Lahmam-Bennani, A.; Duguet, A.

1999-01-01

We report the first measurement of the angular distributions of the two ejected electrons following electron impact double ionization of helium, using a multi-parameter (e,3e) spectrometer. The high incident energy of these experiments allows comparison with photo-double ionization results. Comparison is also made with existing (e,3e) theoretical calculations. (orig.)

Synthesis and structural study on (1E,2E,1'E,2'E)-3,3'-bis[(4-bromophenyl)-3,3'-(4-methy-1,2-phenylene diimine)] acetaldehyde dioxime: A combined experimental and theoretical study

Science.gov (United States)

Topal, T.; Kart, H. H.; Tunay Taşlı, P.; Karapınar, E.

2015-06-01

Tetradentate (1E,2E,1'E,2'E)-3,3'-bis[(4-bromophenyl)-3,3'-(4-methy-1,2-phenylene diimine)] acetaldehyde dioxime which possess N4 donor sets derived from the condensation of isonitroso- p-bromoacetophenone and 3,4-diaminotoluene are synthesized and characterized. The characterization of tetradentate Schiff base ligand has been deduced from LC-MS, FTIR, 13C and 1H NMR spectra and elemental analysis. Furthermore, the molecular geometry, infrared and NMR spectra of the title molecule in the ground state have been calculated by using the quantum chemical computational methods such as density functional theory (DFT) and ab initio Hartree-Fock (HF) methods with the 6-31G(d) and 6-311G(d) basis sets. The computed bond lengths and bond angles by using the both methods show the good agreement with each other. Moreover, the vibrational frequencies have been calculated and the scaled values have been compared with the experimental FTIR spectroscopic data. Assignments of the vibrational modes are made on the basis of potential energy distribution (PED) calculated from by using VEDA program. The correlations between the observed and calculated frequencies are in good agreement with each other as well as the correlation of the NMR data.

Numerical analyses of the effect of SG-interlayer shear stiffness on the structural performance of reinforced glass beams

DEFF Research Database (Denmark)

Louter, C.; Nielsen, Jens Henrik

2013-01-01

This paper focuses on the numerical modelling of SentryGlas-laminated reinforced glass beams. In these beams, which have been experimentally investigated in preceding research, a stainless steel reinforcement section is laminated at the inner recessed edge of a triple-layer glass beam by means...... of SentryGlas (SG) interlayer sheets. The current contribution numerically investigates the effect of the SG-interlayer shear stiffness on the overall structural response of the beams. This is done by means of a 3D finite element model in which the individual glass layers, the SG......-interlayers and the reinforcement are incorporated. In the model, the glass parts are allowed to crack, but all other parts are assumed linear elastic throughout the analyses. By changing the shear modulus of the SG-interlayer in multiple analyses, its contribution to the overall structural performance of the beams - especially...

Force Tests of the Boeing XB-47 Full-Scale Empennage in the Ames 40- by 80-Foot Wind Tunnel

Science.gov (United States)

Hunton, Lynn W.

1947-01-01

A wind-tunnel investigation of the Boeing XB-47 full-scale empennage was conducted to provide, prior to flight tests, data required on the effectiveness of the elevator and rudder. The XB-47 airplane is a jet-propelled medium bomber having wing and tail surfaces swept back 35 degrees. The investigation included tests of the effectiveness of the elevator with normal straight sides, with a buldged trailing edge, and with a modified hinge-line gap and tests of the effectiveness of the rudder with a normal straight-sided tab and with a bulged tab.

Scaling laws in (e,3e) processes

International Nuclear Information System (INIS)

Gasaneo, G; Rodriguez, K V; Ancarani, L U; Cappello, C Dal; Charpentier, I

2009-01-01

We study the double ionization of helium-like ions by impact of electrons with high incident energy. Within the isoelectronic sequence, an approximate scaling law for (e,3e) differential cross sections is proposed and confirmed by calculations. The latter are performed using 14-parameters Hylleraas-like wave functions to represent the bound electrons in the initial channel, plane waves for the fast incoming and scattered electrons, and a continuum distorted wave approach for the two ejected electrons in the final channel.

Measurement of double polarized asymmetries in quasi-elastic processes ${}^3\\vec{He}(\\vec{e},e' d)$ and ${}^3\\vec{He}(\\vec{e},e' p)$

Energy Technology Data Exchange (ETDEWEB)

Mihovilovic, Miha [Univ. of Ljubljana (Slovenia)

2012-01-01

This thesis is dedicated to a study of a spin-isospin structure of the polarized ³He. First, an introduction to the spin structure of ³He is given, followed by a brief overview of past experiments. The main focus of the thesis is the E05-102 experiment at Jefferson Lab, in which the reactions ${}^3\\vec{He}(\\vec{e},e' d)$ and ${}^3\\vec{He}(\\vec{e},e' p)$ in the quasi-elastic region were studied. The purpose of this experiment was to better understand the effects of the S'- and D-state contributions to the ³He ground-state wave-functions by a precise measurement of beam-target asymmetries A_x and A_z in the range of recoil momenta from 0 to about 300 MeV/c. The experimental equipment utilized in these measurements is described, with special attention devoted to the calibration of the hadron spectrometer, BigBite. Results on the measured asymmetries are presented, together with first attempts at their comparison to the state-of-the art Faddeev calculations. The remaining open problems and challenges for future work are also discussed.

E3 Success Story - E3 Southwest Virginia: Economy, Energy and the Environment

Science.gov (United States)

E3 Southwest Virginia supports sustainable manufacturing in 17 counties in southwest Virginia. The MTC provides manufacturers with assessments of production processes to reduce their energy consumption and drive innovation.

E-branding og e-marketing af 3F - et casestudie

OpenAIRE

Brødsgaard, Astrid Isabella; Celik, Nagihan; Johansen, Michelle Lyager

2014-01-01

This project is a case study of the e-branding and e-marketing of 3F, a worker’s union, with a focus on which effect their e-brand has on current and potential members, as well as how successful this e-brand is at attract-ing and keeping members. Some of the aspects explored are the importance of the e-brand’s appearance, the increasing expectations of the members as well as the union’s apparent quality and availability according to its members. This project is a case study of the e-brandi...

An intercomparison of satellite-based daily evapotranspiration estimates under different eco-climatic regions in South Africa

CSIR Research Space (South Africa)

Majozi, Nobuhle P

2017-03-01

Full Text Available ), while windspeed and direction were measured at 2.5 m height using an RM Young wind sentry (Mod l 03001—Campbell Scientific Ltd., Logan, UT, USA), a d solar irradiance was monitored using a pyranometer (Apogee Instru ents, Lo an, UT, USA). R infall... a CS500 probe (Vaisala, Helsinki, Finland), while windspeed and direction were measured at 2.5 m height using an RM Young wind sentry (Model 03001—Campbell Scientific Ltd., Logan, UT, USA), and solar irradiance was monitored using a pyranometer...

Atmospheric chemistry of Z- and E-CF3CH=CHCF3

DEFF Research Database (Denmark)

Østerstrøm, Freja From; Andersen, Simone Thirstrup; Sølling, Theis Ivan

2017-01-01

radicals. The atmospheric lifetimes of Z- and E-CF3CH[double bond, length as m-dash]CHCF3 are estimated as 27 and 67 days, respectively. IR absorption cross sections are reported and the global warming potentials (GWPs) of Z- and E-CF3CH[double bond, length as m-dash]CHCF3 for the 100 year time horizon...

Defense Science Board Task Force Report: Predicting Violent Behavior

Science.gov (United States)

2012-08-01

Boeing • Coca Cola • Disney • Intel Corporation D E F E N S E S C I E N C E B O A R D | D E P A R T M E N T O F D E F E N S E DSB TASK FORCE...University of North Carolina Corporations:  Microsoft  Coca Cola  Boeing  Disney D E F E N S E S C I E N C E B O A R D | D E P A R T M E

Production of e(+)e(-) pairs in proton-deuteron capture to He-3

NARCIS (Netherlands)

Korchin, AY; Van Neck, D; Waroquier, M; Scholten, O; Dieperink, AEL

1998-01-01

The process p + d He-3 + gamma * at intermediate energies is described using a covariant and gauge-invariant model, and a realistic pd(3)He vertex, Both photodisintegration of He-3 and proton-deuteron capture with production of e(+)e(-) pairs are studied, and results for cross sections and response

A review of the application Acoustic Emission (AE) incorporating mechanical approach to monitor Reinforced concrete (RC) strengthened with Fiber Reinforced Polymer (FRP) properties under fracture

Science.gov (United States)

Syed Mazlan, S. M. S.; Abdullah, S. R.; Shahidan, S.; Noor, S. R. Mohd

2017-11-01

Concrete durability may be affected by so many factors such as chemical attack and weathering action that reduce the performance and the service life of concrete structures. Low durability Reinforced concrete (RC) can be greatly improved by using Fiber Reinforce Polymer (FRP). FRP is a commonly used composite material for repairing and strengthening RC structures. A review on application of Acoustic Emission (AE) techniques of real time monitoring for various mechanical tests for RC strengthened with FRP involving four-point bending, three-point bending and cyclic loading was carried out and discussed in this paper. Correlations between each AE analyses namely b-value, sentry and intensity analysis on damage characterization also been critically reviewed. From the review, AE monitoring involving RC strengthened with FRP using b-value, sentry and intensity analysis are proven to be successful and efficient method in determining damage characterization. However, application of AE analysis using sentry analysis is still limited compared to b-value and intensity analysis in characterizing damages especially for RC strengthened with FRP specimen.

3D printed e-tongue

Science.gov (United States)

Gaál, Gabriel; da Silva, Tatiana A.; Gaál, Vladimir; Hensel, Rafael C.; Amaral, Lucas R.; Rodrigues, Varlei; Riul, Antonio

2018-05-01

Nowadays, one of the biggest issues addressed to electronic sensor fabrication is the build-up of efficient electrodes as an alternative way to the expensive, complex and multistage processes required by traditional techniques. Printed electronics arises as an interesting alternative to fulfill this task due to the simplicity and speed to stamp electrodes on various surfaces. Within this context, the Fused Deposition Modeling 3D printing is an emerging, cost-effective and alternative technology to fabricate complex structures that potentiates several fields with more creative ideas and new materials for a rapid prototyping of devices. We show here the fabrication of interdigitated electrodes using a standard home-made CoreXY 3D printer using transparent and graphene-based PLA filaments. Macro 3D printed electrodes were easily assembled within 6 minutes with outstanding reproducibility. The electrodes were also functionalized with different nanostructured thin films via dip-coating Layer-by-Layer technique to develop a 3D printed e-tongue setup. As a proof of concept, the printed e-tongue was applied to soil analysis. A control soil sample was enriched with several macro-nutrients to the plants (N, P, K, S, Mg and Ca) and the discrimination was done by electrical impedance spectroscopy of water solution of the soil samples. The data was analyzed by Principal Component Analysis and the 3D printed sensor distinguished clearly all enriched samples despite the complexity of the soil chemical composition. The 3D printed e-tongue successfully used in soil analysis encourages further investments in developing new sensory tools for precision agriculture and other fields exploiting the simplicity and flexibility offered by the 3D printing techniques.

SUPPLY CHAIN FEATURES OF THE AEROSPACE INDUSTRY PARTICULAR CASE AIRBUS AND BOEING

Directory of Open Access Journals (Sweden)

Daniela MOCENCO

2015-10-01

Full Text Available Aerospace sector is one of the most globalized industries in terms of market structure and production system. Through aircraft development programs aviation industry has introduced new solutions to develop its products. The role of the partners involved in the development programs for the new generation aircraft is becoming increasingly important. Supply chain management has become a key factor for major manufacturers in the industry. The new strategies introduced by the aircraft manufacturers have increased the complexity of the supply process, design and production from the aviation industry. This paper highlights a series of factors regarding the aircraft programs supply chain management. In the first part are described the problems encountered by the manufacturers and airlines during the development of the most recent aircraft launched A350XWB and B787 Dreamliner; the second part focuses on the organizational structure of the supply chain, suppliers role within supply chain and also there are described the effects of the new strategies adopted by Airbus and Boeing in the aircraft development; the last part focuses on the risks and challenges that aviation industry is facing.

Electron-impact excitation of multiply-charged ions using energy loss in merged beams: e + Si3+(3s2S1/2) → e + Si3+(3p2P1/2,3/2)

International Nuclear Information System (INIS)

Wahlin, E.K.; Thompson, J.S.; Dunn, G.H.; Phaneuf, R.A.; Gregory, D.C.; Smith, A.C.H.

1990-01-01

For the first time absolute total cross sections for electron-impact excitation of a multiply-charged ion have been measured using an electron-energy-loss technique. Measurements were made near threshold for the process e + Si 3+ (3s 2 S 1/2 ) → e + Si 3+ (3p 2 P 1/2 , 3/2 ) -- 8.88 eV. The 10 -15 cm 2 measured cross section agrees with results of 7-state close coupling calculations to better than the ±20% (90% CL) total uncertainty of the measurements. Convoluting the theoretical curve with a Gaussian energy distribution indicates an energy width of 0.15 approx-lt ΔE approx-lt 0.20 eV. 12 refs., 2 figs

Coplanar (e, 3e) differential cross-section of He atom

CERN Document Server

Choubisa, R; Sud, K K

2003-01-01

We present in this paper the results of our calculation of five-fold differential cross-section (FDCS) for (e, 3e) process on He atom in low momentum transfer and high electron impact energy in shake-off mechanism. The formalism has been developed in Born approximation using plane waves. Byron and Joachain as well as Le Sech and correlated BBK-type wave functions respectively for incident and scattered. bound and ejected electrons. The angular distribution of FDCS of our calculation is presented in various modes of coplanar geometry and comparison is made with the available experimental data. We observe that the present calculation is able to reproduce the trend of the experimental data. However, it differs in magnitude from the experiment. The present theory does not predict four-peak structure in summed mutual angle mode for lower excess ejected electron energies. We also discuss the importance of momentum transfer, post-collision interaction (PCI) and ion participation in the (e, 3e) process in constant th...

Interfacce e tecnologie visual 3D per conoscere, condividere e valorizzare il patrimonio culturale

Directory of Open Access Journals (Sweden)

Elena Ippoliti

2012-11-01

Full Text Available L’articolo illustra gli esiti della ricerca “Modelli informativi integrati per conoscere, valorizzare e condividere il patrimonio urbano e ambientale. Sperimentare interfacce 3D per oggetti culturali geografici: l'architettura delle informazioni e l'architettura informatica” che si è posta come principale obiettivo quello di dilatare il concetto di “modello informativo integrato”, approfondendolo, attraverso integrazioni e sovrapposizioni con diversi ambienti, nelle direzioni sia dello spazio geografico (3D-GIS e dello spazio Web (3D-WEB e 3D-GIS-WEB, sia della Realtà Aumentata e del Virtuale Aumentato. La ricerca ha mirato ad individuare sistemi tecnologici, procedurali e operativi, diversamente articolati in relazione a casi individuati, privilegiando anche tecnologie basate su strumentazioni di facile uso, a basso costo e/o open source, ma sempre affidabili relativamente alla qualità dei dati elaborati. In tale contesto si sono condotte differenti sperimentazioni, secondo vari percorsi/scale di lettura e corrispondenti organizzazioni dei dati, scegliendo come ambito privilegiato delle applicazioni il centro storico di Ascoli Piceno.

Experiment E89-044 on the Quasielastic ³He(e,e'p) Reaction at Jefferson Laboratory

Energy Technology Data Exchange (ETDEWEB)

Penel-Nottaris, Emilie [Univ. Joseph Fourier Grenoble (France)

2004-07-07

The Jefferson Lab Hall A E89-044 experiment has measured the ³He(e,e'p) reaction cross-sections. The extraction of the longitudinal and transverse response functions for the two-body break-up ³He(e,e'p)d reaction in parallel kinematics allows the study of the bound proton electromagnetic properties inside the ³He nucleus and the involved nuclear mechanisms beyond plane wave approximations.

Status and test report on the LANL-Boeing APLE/HPO flying-wire beam-profile monitor. Status report

Energy Technology Data Exchange (ETDEWEB)

Wilke, M.; Barlow, D.; Fortgang, C.; Gilpatrick, J.; Meyer, R.; Rendon, A.; Warren, D. [Los Alamos National Lab., NM (United States); Greegor, R. [Boeing Co., Seattle, WA (United States)

1994-07-01

The High-Power Oscillator (HPO) demonstration of the Average Power Laser Experiment (APLE) is a collaboration by Los Alamos National Laboratory and Boeing to demonstrate a 10 kW average power, 10 {mu}m free electron laser (FEL). As part of the collaboration, Los Alamos National Laboratory (LANL) is responsible for many of the electron beam diagnostics in the linac, transport, and laser sections. Because of the high duty factor and power of the electron beam, special diagnostics are required. This report describes the flying wire diagnostic required to monitor the beam profile during high-power, high-duty operation. The authors describe the diagnostic and prototype tests on the Los Alamos APLE Prototype Experiment (APEX) FEL. They also describe the current status of the flying wires being built for APLE.

Off-wing fleet maintenance study of a CFM56-3B turbofan engine ...

African Journals Online (AJOL)

An off wing fleet maintenance study of the CFM56-3B Turbofan engine that propels the Boeing 737-300 aircraft is presented. The engine performance and deteriorating behavior was modeled with a view to estimate the creep life consumption and operating severity. The predicted severity factor of each degradation was ...

E3 Success Story - San Antonio E3 Case Study: UEMC “Now Hiring”

Science.gov (United States)

exas Manufacturing Assistance Center conducted an E3 assessment as part of the local Lean-Clean-Energy program in October of 2009. Overall, UEMC has reduced demand by approximately 30kw and reduced consumption by over 100,000 kwh/year.

Molecular structure and spectral properties of ethyl 3-quinolinecarboxylate (E3Q) and [Ag(E3Q)2(TCA)] complex (TCA = Trichloroacetate)

Science.gov (United States)

Soliman, Saied M.; Kassem, Taher S.; Badr, Ahmed M. A.; Abou Youssef, Morsy A.; Assem, Rania

2014-09-01

A new [Ag(E3Q)2(TCA)] complex; (E3Q = Ethyl 3-quinolinecarboxylate and TCA = Trichloroacetate) has been synthesized and characterized using elemental analysis, FTIR, NMR and mass spectroscopy. The molecular geometry and spectroscopic properties of the complex as well as the free ligand have been calculated using the hybrid B3LYP method. The calculations predicted a distorted tetrahedral arrangement around Ag(I) ion. The vibrational spectra of the studied compounds have been assigned using potential energy distribution (PED). TD-DFT method was used to predict the electronic absorption spectra. The most intense absorption band showed a bathochromic shift and lowering of intensity in case of the complex (233.7 nm, f = 0.5604) compared to E3Q (λmax = 228.0 nm, f = 0.9072). The calculated 1H NMR chemical shifts using GIAO method showed good correlations with the experimental data. The computed dipole moment, polarizability and HOMO-LUMO energy gap were used to predict the nonlinear optical (NLO) properties. It is found that Ag(I) enhances the NLO activity. The natural bond orbital (NBO) analyses were used to elucidate the intramolecular charge transfer interactions causing stabilization for the investigated systems.

3D display considerations for rugged airborne environments

Science.gov (United States)

Barnidge, Tracy J.; Tchon, Joseph L.

2015-05-01

The KC-46 is the next generation, multi-role, aerial refueling tanker aircraft being developed by Boeing for the United States Air Force. Rockwell Collins has developed the Remote Vision System (RVS) that supports aerial refueling operations under a variety of conditions. The system utilizes large-area, high-resolution 3D displays linked with remote sensors to enhance the operator's visual acuity for precise aerial refueling control. This paper reviews the design considerations, trade-offs, and other factors related to the selection and ruggedization of the 3D display technology for this military application.

Some nonunitary, indecomposable representations of the Euclidean algebra e(3)

International Nuclear Information System (INIS)

Douglas, Andrew; De Guise, Hubert

2010-01-01

The Euclidean group E(3) is the noncompact, semidirect product group E(3)≅R 3 x SO(3). It is the Lie group of orientation-preserving isometries of three-dimensional Euclidean space. The Euclidean algebra e(3) is the complexification of the Lie algebra of E(3). We construct three distinct families of finite-dimensional, nonunitary representations of e(3) and show that each representation is indecomposable. The representations of the first family are explicitly realized as subspaces of the polynomial ring F[X,Y,Z] with the action of e(3) given by differential operators. The other families are constructed via duals and tensor products of the representations within the first family. We describe subrepresentations, quotients and duals of these indecomposable representations.

Structure of eEF3 and the mechanism of transfer RNA release from the E-site

DEFF Research Database (Denmark)

Andersen, Christian Brix Folsted; Becker, T.; Blau, M.

2006-01-01

Elongation factor eEF3 is an ATPase, which, in addition to the two canonical factors eEF1A and eEF2, serves an essential function in the translation cycle of fungi. eEF3 is required for the binding of the aatRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has been suggested to facilitate...... the clearance of deacyl-tRNA from the E-site. Here, we present the crystal structure of eEF3 showing that it consists of an N-terminal HEAT repeat domain, followed by a four-helix bundle and two ABC-type ATPase domains with a chromo-domain inserted in ABC2. Moreover, we present the cryo-EM structure of the ATP......-bound form of eEF3 in complex with the post-translocational state 80S ribosome from yeast. eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain stabilizing the ribosomal L1 stalk in an open conformation, thus, allowing tRNA release....

Flex-prints for the Mu3e experiment

Energy Technology Data Exchange (ETDEWEB)

Dittmeier, Sebastian [Physikalisches Institut, Universitaet Heidelberg (Germany); Collaboration: Mu3e-Collaboration

2016-07-01

Mu3e is a novel experiment dedicated to the search for the charged lepton flavor violating decay μ{sup +} → e{sup +}e{sup -}e{sup +} with a targeted sensitivity of a branching ratio (BR) down to 10{sup -16}. Within the standard model of particle physics this process is extremely suppressed to BR<10{sup -54}. Thus, any observation would be a clear sign for new physics beyond the standard model. The Mu3e detector consists of a silicon pixel tracking detector using the novel HV-MAPS (High Voltage Monolithic Active Pixel Sensor) technology to measure the momentum of the decay products, and scintillating fibres and tiles for precise timing. The pixel sensors are thinned to 50 μ m to achieve a material budget of about 0.1% radiation length per tracking layer. The pixel sensors are powered and connected to readout frontend boards using custom designed flex-prints, which are low material interconnects with a high signal density. We present studies of high speed data transmission up to 3.2 Gb/s over flex-print prototypes produced in-house. The design of the flex-prints for the Mu3e detector will be discussed. A 3-layer design including power, ground and slow control distribution, as well as 9 1.25 Gb/s data transmission lines for the readout of the pixel sensors is foreseen.

Scaling of cross-sections for asymmetric (e,3e) process on helium ...

Indian Academy of Sciences (India)

, India ... (e, 3e) process; five-fold differential cross-section; scaling; helium isoelec- tronic ions. ... ration and experimental control of the target and the intensity related problems make the measurements extremely difficult. The scaling laws of ...

Bis{(E-3-[2-(hydroxyiminopropanamido]-2,2-dimethylpropan-1-aminium} bis[μ-(E-N-(3-amino-2,2-dimethylpropyl-2-(hydroxyiminopropanamido(2−]bis{[(E-N-(3-amino-2,2-dimethylpropyl-2-(hydroxyiminopropanamide]copper(II} bis((E-{3-[2-(hydroxyiminopropanamido]-2,2-dimethylpropyl}carbamate acetonitrile disolvate

Directory of Open Access Journals (Sweden)

Andrii I. Buvailo

2012-12-01

Full Text Available The reaction between copper(II nitrate and (E-N-(3-amino-2,2-dimethylpropyl-2-(hydroxyiminopropanamide led to the formation of the dinuclear centrosymmetric copper(II title complex, (C8H18N3O22[Cu2(C8H15N3O22(C8H17N3O22](C9H16N3O42·2CH3CN, in which an inversion center is located at the midpoint of the Cu2 unit in the center of the neutral [Cu2(C8H15N3O22(C8H17N3O22] complex fragment. The Cu2+ ions are connected by two N—O bridging groups [Cu...Cu separation = 4.0608 (5 Å] while the CuII ions are five-coordinated in a square-pyramidal N4O coordination environment. The complex molecule co-crystallizes with two molecules of acetonitrile, two molecules of the protonated ligand (E-3-[2-(hydroxyiminopropanamido]-2,2-dimethylpropan-1-aminium and two negatively charged (E-{3-[2-(hydroxyiminopropanamido]-2,2-dimethylpropyl}carbamate anions, which were probably formed as a result of condensation between (E-N-(3-amino-2,2-dimethylpropyl-2-(hydroxyiminopropanamide and hydrogencarbonate anions. In the crystal, the complex fragment [Cu2(C8H15N3O22(C8H17N3O22] and the ion pair C8H18N3O2+.C9H16N3O4− are connected via an extended system of hydrogen bonds.

(E-3-Methyl-6-(3-oxo-3-(thiophen-2-yl-1-propenyl-2(3H-benzothiazolone

Directory of Open Access Journals (Sweden)

Yordanka Ivanova

2016-04-01

Full Text Available The title compound, (E-3-methyl-6-(3-oxo-3-(thiophen-2-yl-1-propenyl-2(3H-benzothiazolone, was synthesized by Claisen-Schmidt condensation of 3-methyl-2(3H-benzothiazolone-6-carbaldehyde with 2-acetylthiophene in 94% yield. The structure of the target compound was confirmed using 1H-NMR, 13C-NMR, IR, MS, and elemental analysis.

Oct3/4 directly regulates expression of E2F3a in mouse embryonic stem cells

International Nuclear Information System (INIS)

Kanai, Dai; Ueda, Atsushi; Akagi, Tadayuki; Yokota, Takashi; Koide, Hiroshi

2015-01-01

Embryonic stem (ES) cells, derived from the inner cell mass of blastocysts, have a characteristic cell cycle with truncated G1 and G2 phases. Recent findings that suppression of Oct3/4 expression results in a reduced proliferation rate of ES cells suggest the involvement of Oct3/4 in the regulation of ES cell growth, although the underlying molecular mechanism remains unclear. In the present study, we identified E2F3a as a direct target gene of Oct3/4 in ES cells. Oct3/4 directly bound to the promoter region of the E2F3a gene and positively regulated expression of E2F3a in mouse ES cells. Suppression of E2F3a activity by E2F6 overexpression led to the reduced proliferation in ES cells, which was relieved by co-expression of E2F3a. Furthermore, cell growth retardation caused by loss of Oct3/4 was rescued by E2F3a expression. These results suggest that Oct3/4 upregulates E2F3a expression to promote ES cell growth. - Highlights: • Oct3/4 positively regulates E2F3a expression in ES cells. • Oct3/4 binds to the promoter region of the E2F3a gene. • Overexpression of E2F6, an inhibitor of E2F3a, reduces ES cell growth. • E2F3a recovers growth retardation of ES cells caused by Oct3/4 reduction

Oct3/4 directly regulates expression of E2F3a in mouse embryonic stem cells

Energy Technology Data Exchange (ETDEWEB)

Kanai, Dai; Ueda, Atsushi; Akagi, Tadayuki; Yokota, Takashi; Koide, Hiroshi, E-mail: hkoide@med.kanazawa-u.ac.jp

2015-04-10

Embryonic stem (ES) cells, derived from the inner cell mass of blastocysts, have a characteristic cell cycle with truncated G1 and G2 phases. Recent findings that suppression of Oct3/4 expression results in a reduced proliferation rate of ES cells suggest the involvement of Oct3/4 in the regulation of ES cell growth, although the underlying molecular mechanism remains unclear. In the present study, we identified E2F3a as a direct target gene of Oct3/4 in ES cells. Oct3/4 directly bound to the promoter region of the E2F3a gene and positively regulated expression of E2F3a in mouse ES cells. Suppression of E2F3a activity by E2F6 overexpression led to the reduced proliferation in ES cells, which was relieved by co-expression of E2F3a. Furthermore, cell growth retardation caused by loss of Oct3/4 was rescued by E2F3a expression. These results suggest that Oct3/4 upregulates E2F3a expression to promote ES cell growth. - Highlights: • Oct3/4 positively regulates E2F3a expression in ES cells. • Oct3/4 binds to the promoter region of the E2F3a gene. • Overexpression of E2F6, an inhibitor of E2F3a, reduces ES cell growth. • E2F3a recovers growth retardation of ES cells caused by Oct3/4 reduction.

Plutonium-burn high temperature gas-cooled reactor for 3E+3S

International Nuclear Information System (INIS)

Okamoto, Koji

2015-01-01

The Nuclear Energy Development in Japan is facing a very difficult conditions after Fukushima-Daiichi NPP Accident. Nuclear Energy has strong advantages on 3E, i.e., Energy security, Economical efficiency and Environment. However, people does not believe the Safety 'S' of Nuclear Energy, now. The disadvantage of 'S' overrides the advantages of '3E'. In Nuclear Energy, 'S' is expanded into 3S, i.e., Safety, Security and Safeguards. Especially, the management of Plutonium inventory in Spent Fuel generated by the NPP operation is very important in the viewpoints of non-proliferation. The high-temperature gas cooled reactor (HTGR) is the solution of these disadvantages of '3S' in Nuclear Energy. The fuel of HTGR is composed by 1 mm spherical fuel particle, i.e., TRISO made by fuel, graphite and silicon-carbide. The silicon-carbide can confine the fission products in any conditions of fuel life cycle, i.e., during operation, accidents and disposal for 1 million years. The confinement of the radioactive materials can be confirmed by the TRISO. The HTGR core has strong negative feedback for temperature. So, the fission automatically stopped at the accidental conditions, such as loss of flow and LOCA. Also, the residual heat can be cooled by the radiation heat transfer to reactor vessel wall. The HTGR system usually has passive vessel wall cooling system. When the passive cooling system had been failed, the heat can be transferred to the land by heat conductions, and fuel does not reach the SiC broken temperature. The fission chain reaction has been stopped automatically by negative feedback, i.e., physics. The residual heat had been cooled automatically by radiation. The radioactive materials had been confined automatically by silicon-carbide. The HTGR is superior for 'S' safety. Plutonium can be burned by the HTGR. In the viewpoints of non-proliferation, the fuel should be made by YSZ-PuO 2 , stabilized buffer

The Mu3e Tile Detector

Energy Technology Data Exchange (ETDEWEB)

Eckert, Hans Patrick

2015-05-06

The Mu3e experiment is designed to search for the lepton flavour violating decay μ→e{sup +}e{sup +}e{sup -} with a sensitivity of one in 10{sup 16} decays. An observation of such a decay would be a clear sign of physics beyond the Standard Model. Achieving the targeted sensitivity requires a high precision detector with excellent momentum, vertex and time resolution. The Mu3e Tile Detector is a highly granular sub-detector system based on scintillator tiles with Silicon Photomultiplier (SiPM) readout, and aims at measuring the timing of the muon decay products with a resolution of better than 100 ps. This thesis describes the development of the Tile Detector concept and demonstrates the feasibility of the elaborated design. In this context, a comprehensive simulation framework has been developed, in order to study and optimise the detector performance. The central component of this framework is a detailed simulation of the SiPM response. The simulation model has been validated in several measurements and shows good agreement with the data. Furthermore, a 16-channel prototype of a Tile Detector module has been constructed and operated in an electron beam. In the beam tests, a time resolution up to 56 ps has been achieved, which surpasses the design goal. The simulation and measurement results demonstrate the feasibility of the developed Tile Detector design and show that the required detector performance can be achieved.

E3 Success Story - Working Together: E3 Ohio and the Ohio By-Product Synergy Network

Science.gov (United States)

The Mid-Ohio Regional Planning Commission (MORPC) received funding to support the integration of the national E3 sustainability initiative with the Ohio By-Product Synergy (BPS) Network to create an efficient and replicable model for reducing GHGs.

Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability

Energy Technology Data Exchange (ETDEWEB)

Mei, Ya-Fang, E-mail: ya-fang.mei@umu.se [Department of Clinical Microbiology and Virology, Umeå University, SE-901 85 Umeå (Sweden); Wu, Haidong, E-mail: haidong.wu@umu.se [Department of Clinical Microbiology and Virology, Umeå University, SE-901 85 Umeå (Sweden); Hultenby, Kjell, E-mail: kjell.hultenby@ki.se [Division of Clinical Research Centre, Department of Laboratory Medicine, Karolinska Institute, SE-14186 Stockholm (Sweden); Silver, Jim, E-mail: jim.silver@umu.se [Department of Clinical Microbiology and Virology, Umeå University, SE-901 85 Umeå (Sweden)

2016-10-15

Conventional adenovirus vectors harboring E1 or E3 deletions followed by the insertion of an exogenous gene show considerably reduced virion stability. Here, we report strategies to generate complete replication-competent Ad11p(RCAd11p) vectors that overcome the above disadvantage. A GFP cassette was successfully introduced either upstream of E1A or in the E3A region. The resulting vectors showed high expression levels of the hexon and E1genes and also strongly induced the cytopathic effect in targeted cells. When harboring oversized genomes, the RCAd11pE1 and RCAd11pE3 vectors showed significantly improved heat stability in comparison to Ad11pwt;of the three, RCAd11pE3 was the most tolerant to heat treatment. Electron microscopy showed that RCAd11pE3, RCAd11pE1, Ad11pwt, and Ad11pE1 Delmanifested dominant, moderate, minimum, or no full virus particles after heat treatment at 47 °C for 5 h. Our results demonstrated that both genome size and the insertion site in the viral genome affect virion stability. -- Highlights: •Replicating adenovirus 11p GFP vectors at the E1 or E3 region were generated. •RCAd11pE3 and RCAd11pE1 vectors manifested significantly improved heat stability. •RCAd11pE3 and RCAd11pE1 showed more full viral particles than Ad11pwt after heating. •We demonstrated that both genome size and the insertion site affect virion stability.

Complete replication-competent adenovirus 11p vectors with E1 or E3 insertions show improved heat stability

International Nuclear Information System (INIS)

Mei, Ya-Fang; Wu, Haidong; Hultenby, Kjell; Silver, Jim

2016-01-01

Conventional adenovirus vectors harboring E1 or E3 deletions followed by the insertion of an exogenous gene show considerably reduced virion stability. Here, we report strategies to generate complete replication-competent Ad11p(RCAd11p) vectors that overcome the above disadvantage. A GFP cassette was successfully introduced either upstream of E1A or in the E3A region. The resulting vectors showed high expression levels of the hexon and E1genes and also strongly induced the cytopathic effect in targeted cells. When harboring oversized genomes, the RCAd11pE1 and RCAd11pE3 vectors showed significantly improved heat stability in comparison to Ad11pwt;of the three, RCAd11pE3 was the most tolerant to heat treatment. Electron microscopy showed that RCAd11pE3, RCAd11pE1, Ad11pwt, and Ad11pE1 Delmanifested dominant, moderate, minimum, or no full virus particles after heat treatment at 47 °C for 5 h. Our results demonstrated that both genome size and the insertion site in the viral genome affect virion stability. -- Highlights: •Replicating adenovirus 11p GFP vectors at the E1 or E3 region were generated. •RCAd11pE3 and RCAd11pE1 vectors manifested significantly improved heat stability. •RCAd11pE3 and RCAd11pE1 showed more full viral particles than Ad11pwt after heating. •We demonstrated that both genome size and the insertion site affect virion stability.

Calcification of MC3T3-E1 cells on titanium and zirconium.

Science.gov (United States)

Umezawa, Takayuki; Chen, Peng; Tsutsumi, Yusuke; Doi, Hisashi; Ashida, Maki; Suzuki, Shoichi; Moriyama, Keiji; Hanawa, Takao

2015-01-01

To confirm similarity of hard tissue compatibility between titanium and zirconium, calcification of MC3T3-E1 cells on titanium and zirconium was evaluated in this study. Mirror-polished titanium (Ti) and zirconium (Zr) disks and zirconium-sputter deposited titanium (Zr/Ti) were employed in this study. The surface of specimens were characterized using scanning electron microscopy and X-ray diffraction. Then, the cellular proliferation, differentiation and calcification of MC3T3-E1 cells on specimens were investigated. The surface of Zr/Ti was much smoother and cleaner than those of Ti and Zr. The proliferation of the cell was the same among three specimens, while the differentiation and calcification on Zr/Ti were faster than those on Ti and Zr. Therefore, Ti and Zr showed the identical hard tissue compatibility according to the evaluation with MC3T3-E1 cells. Sputter deposition may improve cytocompatibility.

(E-3-Methyl-6-(3-oxo-3-(3,4,5-trimethoxyphenylprop-1-en-1-yl-2(3H-benzothiazolone

Directory of Open Access Journals (Sweden)

Yordanka Ivanova

2016-09-01

Full Text Available The title compound, (E-3-methyl-6-(3-oxo-3-(3,4,5-trimethoxyphenylprop-1-en-1-yl-2(3H-benzothiazolone, was synthesized by both an acid- and base-catalyzed aldol condensation of 3-methyl-6-acetyl-2(3H-benzothiazolone and 3,4,5-trimethoxyacetophenone. The structure of the target compound was confirmed using 1H-NMR, 13C-NMR, IR, MS, and elemental analysis.

Características psicométricas e dados normativos do Teste das Três Palavras e Três Figuras (3P3F no Brasil

Directory of Open Access Journals (Sweden)

Moura, Samara Melo

2008-01-01

Full Text Available O Teste das Três Palavras e Três Figuras (3P3F é um instrumento de avaliação rápida e multidimensional da memória episódica, desenvolvido para idosos. Ele ocuparia posição intermediária entre o item de memória do Mini Exame do Estado Mental (MEEM e tarefas mais complexas de listas de figuras e palavras. As características psicométricas de uma adaptação brasileira do 3P3F para amostras de 33 portadores de doença de Alzheimer (DA e 99 idosos cognitivamente normais demograficamente pareados foram investigadas. Adicionalmente, normas de desempenho foram desenvolvidas em um grupo de 350 idosos saudáveis. Os resultados revelam alta confiabilidade (a = 0,89 e poder descriminativo para a identificação das desordens cognitivas associadas à DA (ASC = 0,98; sensibilidade/especificidade = 94,9/92,3%. Observou-se a correlação do 3P3f com o MEEM e a Bateria de Avaliação Frontal (r = 0,65 e r = 0,46; p <0,05. Um modelo de regressão logística sugere a influência da idade e da escolaridade sobre os escores dos testes (B = 0,06 e B = 0,13; P <0,05. Os resultados sugerem adequação psicométrica do instrumento

E3: Economy, Energy and Environment

Science.gov (United States)

E3 is a technical assistance framework helping communities, manufacturers, and manufacturing supply chains adapt and thrive in today's green economy. Find information on pollution prevention, sustainable business practices, and energy efficiency.

E3 Travel & Mission Support System

Data.gov (United States)

US Agency for International Development — ETRAMS is a travel data collection system developed by the CKM team in E3 that collects information on both the basic details of an employee's trips (destination,...

Phillips Petroleum -- Capitalizing on technology

International Nuclear Information System (INIS)

Perdue, J.M.

1996-01-01

Phillips Petroleum Co., one of the largest international oil companies and the largest producer of natural gas liquids in the US, recently reported that first half 1996 net income was $916 million, up from $224 million for the same period the year before. During the past 5 years, reserves have increased 11% and production by 8%. Exploration expenses are down 32% and production costs per boe are down 24%. Compared to other majors involved in worldwide E and P, Phillips has the second highest 5-year percentage of reserves replacement--117%--and the second lowest finding and development cost/boe--$3.46--in the industry. How did the company do this while decreasing headcount by 5,300? The paper discusses focused growth, paring down assets, E and P projects, research and development, and electronic document management

77 FR 65767 - Petition for Exemption From the Federal Motor Vehicle Theft Prevention Standard; Chrysler

Science.gov (United States)

2012-10-30

... (RFHM), Ignition Node Module (IGNM), Engine Control Module, Body Controller Module, Sentry Key... disable engine operation and immobilize the vehicle after two seconds of running. This process is also...

3He(e,e'p)2H breakup process

International Nuclear Information System (INIS)

van Meijgaard, E.; Tjon, J.A.

1990-04-01

The coincidence cross section of the electron-induced two-body breakup reaction 3 He(e,e'p)d is studied at various kinematic configurations. Nucleonic final-state interactions are treated exactly by solving the Faddeev equations for the relevant scattering states. The essential kinematic parameter in analyzing the results for the various kinematic regions is the missing momentum of the struck nucleon. At missing momenta below 250 MeV/c the s-wave analysis gives an adequate description of the experimental data. At missing momenta beyond 350 MeV/c a pure s-wave analysis is not sufficient. Contributions from the d-state components in the trinucleon wave functions to the disconnected graphs are considered. (Author) 12 refs., 15 figs., tab

The Orf virus E3L homologue is able to complement deletion of the vaccinia virus E3L gene in vitro but not in vivo

International Nuclear Information System (INIS)

Vijaysri, Sangeetha; Talasela, Latha; Mercer, Andrew A.; Mcinnes, Colin J.; Jacobs, Bertram L.; Langland, Jeffrey O.

2003-01-01

Orf virus (OV), the prototypic parapoxvirus, is resistant to the effects of interferon (IFN) and this function of OV has been mapped to the OV20.0L gene. The protein product of this gene shares 31% amino acid identity to the E3L-encoded protein of vaccinia virus (VV) that is required for the broad host range and IFN-resistant phenotype of VV in cells in culture and for virulence of the virus in vivo. In this study we investigated whether the distantly related OV E3L homologue could complement the deletion of E3L in VV. The recombinant VV (VV/ORF-E3L) expressing the OV E3L homologue in place of VV E3L was indistinguishable from wt VV in its cell-culture phenotype. But VV/ORF-E3L was over a 1000-fold less pathogenic than wt VV (LD 50 > 5 x 10 6 PFU, compared to LD 50 of wtVV = 4 x 10 3 PFU) following intranasal infection of mice. While wt VV spread to the lungs and brain and replicated to high titers in the brain of infected mice, VV/ORF-E3L could not be detected in the lungs or brain following intranasal infection. VV/ORF-E3L was at least 100,000-fold less pathogenic than wt VV on intracranial injection. Domain swap experiments demonstrate that the difference in pathogenesis maps to the C-terminal domain of these proteins. This domain has been shown to be required for the dsRNA binding function of the VV E3L

Biomarkers of exposure in environment-wide association studies - Opportunities to decode the exposome using human biomonitoring data.

Science.gov (United States)

Steckling, Nadine; Gotti, Alberto; Bose-O'Reilly, Stephan; Chapizanis, Dimitris; Costopoulou, Danae; De Vocht, Frank; Garí, Mercè; Grimalt, Joan O; Heath, Ester; Hiscock, Rosemary; Jagodic, Marta; Karakitsios, Spyros P; Kedikoglou, Kleopatra; Kosjek, Tina; Leondiadis, Leondios; Maggos, Thomas; Mazej, Darja; Polańska, Kinga; Povey, Andrew; Rovira, Joaquim; Schoierer, Julia; Schuhmacher, Marta; Špirić, Zdravko; Stajnko, Anja; Stierum, Rob; Tratnik, Janja Snoj; Vassiliadou, Irene; Annesi-Maesano, Isabella; Horvat, Milena; Sarigiannis, Dimosthenis A

2018-07-01

The European Union's 7th Framework Programme (EU's FP7) project HEALS - Health and Environment-wide Associations based on Large Population Surveys - aims a refinement of the methodology to elucidate the human exposome. Human biomonitoring (HBM) provides a valuable tool for understanding the magnitude of human exposure from all pathways and sources. However, availability of specific biomarkers of exposure (BoE) is limited. The objective was to summarize the availability of BoEs for a broad range of environmental stressors and exposure determinants and corresponding reference and exposure limit values and biomonitoring equivalents useful for unraveling the exposome using the framework of environment-wide association studies (EWAS). In a face-to-face group discussion, scope, content, and structure of the HEALS deliverable "Guidelines for appropriate BoE selection for EWAS studies" were determined. An expert-driven, distributed, narrative review process involving around 30 individuals of the HEALS consortium made it possible to include extensive information targeted towards the specific characteristics of various environmental stressors and exposure determinants. From the resulting 265 page report, targeted information about BoE, corresponding reference values (e.g., 95th percentile or measures of central tendency), exposure limit values (e.g., the German HBM I and II values) and biomonitoring equivalents (BEs) were summarized and updated. 64 individual biological, chemical, physical, psychological and social environmental stressors or exposure determinants were included to fulfil the requirements of EWAS. The list of available BoEs is extensive with a number of 135; however, 12 of the stressors and exposure determinants considered do not leave any measurable specific substance in accessible body specimens. Opportunities to estimate the internal exposure stressors not (yet) detectable in human specimens were discussed. Data about internal exposures are useful to decode

Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle's dynamics and signaling.

Directory of Open Access Journals (Sweden)

Wei Li

2008-01-01

Full Text Available Specificity of protein ubiquitylation is conferred by E3 ubiquitin (Ub ligases. We have annotated approximately 617 putative E3s and substrate-recognition subunits of E3 complexes encoded in the human genome. The limited knowledge of the function of members of the large E3 superfamily prompted us to generate genome-wide E3 cDNA and RNAi expression libraries designed for functional screening. An imaging-based screen using these libraries to identify E3s that regulate mitochondrial dynamics uncovered MULAN/FLJ12875, a RING finger protein whose ectopic expression and knockdown both interfered with mitochondrial trafficking and morphology. We found that MULAN is a mitochondrial protein - two transmembrane domains mediate its localization to the organelle's outer membrane. MULAN is oriented such that its E3-active, C-terminal RING finger is exposed to the cytosol, where it has access to other components of the Ub system. Both an intact RING finger and the correct subcellular localization were required for regulation of mitochondrial dynamics, suggesting that MULAN's downstream effectors are proteins that are either integral to, or associated with, mitochondria and that become modified with Ub. Interestingly, MULAN had previously been identified as an activator of NF-kappaB, thus providing a link between mitochondrial dynamics and mitochondria-to-nucleus signaling. These findings suggest the existence of a new, Ub-mediated mechanism responsible for integration of mitochondria into the cellular environment.

Structure of the Human FANCL RING-Ube2T Complex Reveals Determinants of Cognate E3-E2 Selection

Science.gov (United States)

Hodson, Charlotte; Purkiss, Andrew; Miles, Jennifer Anne; Walden, Helen

2014-01-01

Summary The combination of an E2 ubiquitin-conjugating enzyme with an E3 ubiquitin-ligase is essential for ubiquitin modification of a substrate. Moreover, the pairing dictates both the substrate choice and the modification type. The molecular details of generic E3-E2 interactions are well established. Nevertheless, the determinants of selective, specific E3-E2 recognition are not understood. There are ∼40 E2s and ∼600 E3s giving rise to a possible ∼24,000 E3-E2 pairs. Using the Fanconi Anemia pathway exclusive E3-E2 pair, FANCL-Ube2T, we report the atomic structure of the FANCL RING-Ube2T complex, revealing a specific and extensive network of additional electrostatic and hydrophobic interactions. Furthermore, we show that these specific interactions are required for selection of Ube2T over other E2s by FANCL. PMID:24389026

E3: Extreme Energy Event monitoring

CERN Document Server

CERN. Geneva

2015-01-01

World peace through CERN technology: Use of explosives especially in urban areas is the defining phenomenon of the last and current century. E3 aims to reduce such excesses of violence by collecting scientific hard evidence.

Aspects of radiative K+e3 decays

International Nuclear Information System (INIS)

Kubis, B.; Mueller, E.H.; Gasser, J.; Schmid, M.

2007-01-01

We re-investigate the radiative charged kaon decay K ± →π 0 e ± ν e γ [K e3γ ± ] in chiral perturbation theory, merging the chiral expansion with Low's theorem. We thoroughly analyze the precision of the predicted branching ratio relative to the non-radiative decay channel. Structure dependent terms and their impact on differential decay distributions are investigated in detail, and the possibility to see effects of the chiral anomaly in this decay channel is emphasized. (orig.)

Molecular docking studies of (1E,3E,5E)-1,6-Bis(substituted phenyl)hexa-1,3,5-triene and 1,4-Bis(substituted trans-styryl)benzene analogs as novel tyrosinase inhibitors.

Science.gov (United States)

Ha, Young Mi; Lee, Hye Jin; Park, Daeui; Jeong, Hyoung Oh; Park, Ji Young; Park, Yun Jung; Lee, Kyung Jin; Lee, Ji Yeon; Moon, Hyung Ryong; Chung, Hae Young

2013-01-01

We simulated the docking of the tertiary structure of mushroom tyrosinase with our compounds. From the structure-tyrosinase inhibitory activity relationship, it is notable that compounds 4, 8 and 11 showed similar or better activity rates than kojic acid which was used as a positive control. Compounds 17, 21, and 23 among benzene analogs that possess the same substituent showed significantly lower tyrosinase inhibitory effects. Therefore, we have confirmed that among the compounds showing better tyrosinase inhibitory effects than kojic acid, the compounds with triene analogs have better tyrosinase inhibitory effect than the compounds with benzene analogs. Docking simulation suggested the mechanism of compounds by several key residues which had possible hydrogen bonding interactions. The pharmacophore model underlined the features of active compounds, 4,4'-((1E,3E,5E)-hexa-1,3,5-triene-1,6-diyl)diphenol, 5,5'-((1E,3E,5E)-hexa-1,3,5-triene-1,6-diyl)bis(2-methoxy-phenol), and 5,5'-((1E,3E,5E)-hexa-1,3,5-triene-1,6-diyl)dibenzene-1,3-diol among triene derivatives which had several hydrogen bond groups on both terminal rings. The soundness of the docking results and the agreement with the pharmacophores suggest that it can be conveniently exploited to design inhibitors with an improved affinity for tyrosinase.

E3 Financing How-to Guide

Science.gov (United States)

The Financing How-to-Guide is intended to help manufacturers and their communities navigate financing and investment opportunities. While this guide provides an overview, there is no one-way to pay for E3 activities or attract investment.

Divalent Metal Ions Induced Osteogenic Differentiation of MC3T3E1

Science.gov (United States)

Wang, Guoshou; Su, Wenta; Chen, Pohung; Huang, Teyang

2017-12-01

Biomaterial scaffolds blended with biochemical signal molecules with adequate osteoinductive and osteoconductive properties have attracted significant interest in bone tissue engineering regeneration. The divalent metal ions can gradually release from the scaffold into the culture medium and then induced osteoblastic differentiation of MC3T3E1. These MC3T3E1 cells expressed high activity of alkaline phosphatase, bone-related gene expression of collagen type I, Runx2, osteopontin, osteocalcin, and significantly enhanced deposited minerals on scaffold after 21 days of culture. This experiment provided a useful inducer for osteogenic differentiation in bone repair.

Synthesis of a tritium labeled photolabile analogue of farnesyl diphosphate: (E,E)-[1-3H]-(2-diazo-3-trifluoropropionyloxy)geranyl diphosphate (DATFP-GDP)

International Nuclear Information System (INIS)

Liu, J.; Benedict, C.R.

1996-01-01

Tritiated (E,E)-(2-diazo-3-trifluoropropionyloxy)geranyl disphosphate (DATFP-GDP) has been used as a photolabile analogue of (E,E)-farnesyl diphosphate (E,E-FDP) for an aid in isolating enzymes utilizing E,E-FDP as a substrate. We now report an alternative method of synthesizing this probe in which the tritium label is introduced in the step just before the introduction of the diphospate group. Thus, DATFP-geranial is oxidized to DATFP-geranial with activated manganese dioxide. The tritium label is introduced by reduction of the aldehyde with NaBT 4 . The DATFP-group successfully withstands both of these steps. The overall yield for these two steps is 69%. Diphosphorylation of the resulting alcohol afforded DATFP-[1- 3 H]-GDP in 8% yield with a specific activity of 48.6 μCi/μmol and radiochemical purity of 94%. (Author)

Site Environmental Report for Calendar Year 1999. DOE Operations at The Boeing Company, Rocketdyne

Energy Technology Data Exchange (ETDEWEB)

None

2000-09-01

OAK A271 Site Environmental Report for Calendar Year 1999. DOE Operations at The Boeing Company, Rocketdyne. This Annual Site Environmental Report (ASER) for 1999 describes the environmental conditions related to work performed for the Department of Energy (DOE) at Area IV of the Rocketdyne Santa Susana Field Laboratory (SSFL). In the past, these operations included development, fabrication, and disassembly of nuclear reactors, reactor fuel, and other radioactive materials under the former Atomics International Division. Other activities included the operation of large-scale liquid metal facilities for testing of liquid metal fast breeder components at the Energy Technology Engineering Center (ETEC), a government-owned, company-operated test facility within Area IV. All nuclear work was terminated in 1988, and subsequently, all radiological work has been directed toward decontamination and decommissioning (D&D) of the previously used nuclear facilities and associated site areas. Large-scale D&D activities of the sodium test facilities began in 1996. This Annual Site Environmental Report provides information showing that there are no indications of any potential impact on public health and safety due to the operations conducted at the SSFL. All measures and calculations of off-site conditions demonstrate compliance with applicable regulations, which provide for protection of human health and the environment.

An Analysis of 20 Years of Space Weathering Effects on the Boeing 376 Spacecraft

Science.gov (United States)

Frith, James; Anz-Meador, Phillip; Cowardin, Heather; Buckalew, Brent; Lederer, Susan

2015-01-01

The Boeing HS-376 spin stabilized spacecraft was a popular design that was launched continuously into geosynchronous orbit starting in 1980, with the last launch occurring in 2003. Over 50 of the HS-376 buses were produced to fulfill a variety of different communication missions for countries all over the world. The design of the bus is easily approximated as a telescoping cylinder that is covered with solar cells and an Earth-facing antenna that is despun at the top of the cylinder. The similarity in design and the number of spacecraft launched over a long period of time make the HS-376 a prime target for studying the effects of solar weathering on solar panels as a function of time. A selection of primarily non-operational HS-376 spacecraft launched over a 20-year time period were observed using the United Kingdom Infrared Telescope on Mauna Kea and multi-band, near-infrared photometry produced. Each spacecraft was observed for an entire night cycling through ZYJHK filters and time-varying colors produced to compare near-infrared color as a function of launch date. The resulting analysis shown here may help in the future to set launch date constraints on the parent object of unidentified debris objects or other unknown spacecraft.

Site Environmental Report for Calendar Year 1999. DOE Operations at The Boeing Company, Rocketdyne

International Nuclear Information System (INIS)

None

2000-01-01

OAK A271 Site Environmental Report for Calendar Year 1999. DOE Operations at The Boeing Company, Rocketdyne. This Annual Site Environmental Report (ASER) for 1999 describes the environmental conditions related to work performed for the Department of Energy (DOE) at Area IV of the Rocketdyne Santa Susana Field Laboratory (SSFL). In the past, these operations included development, fabrication, and disassembly of nuclear reactors, reactor fuel, and other radioactive materials under the former Atomics International Division. Other activities included the operation of large-scale liquid metal facilities for testing of liquid metal fast breeder components at the Energy Technology Engineering Center (ETEC), a government-owned, company-operated test facility within Area IV. All nuclear work was terminated in 1988, and subsequently, all radiological work has been directed toward decontamination and decommissioning (D and D) of the previously used nuclear facilities and associated site areas. Large-scale D and D activities of the sodium test facilities began in 1996. This Annual Site Environmental Report provides information showing that there are no indications of any potential impact on public health and safety due to the operations conducted at the SSFL. All measures and calculations of off-site conditions demonstrate compliance with applicable regulations, which provide for protection of human health and the environment

76 FR 68260 - Petition for Exemption From the Federal Motor Vehicle Theft Prevention Standard; Chrysler

Science.gov (United States)

2011-11-03

...), Ignition Node Module (IGNM), Engine Control Module (ECM), Body Controller Module (BCM), Sentry Key... and immobilize the vehicle after two seconds of running. This process is also similar for the keyless...

Dosage-dependent copy number gains in E2f1 and E2f3 drive hepatocellular carcinoma.

Science.gov (United States)

Kent, Lindsey N; Bae, Sooin; Tsai, Shih-Yin; Tang, Xing; Srivastava, Arunima; Koivisto, Christopher; Martin, Chelsea K; Ridolfi, Elisa; Miller, Grace C; Zorko, Sarah M; Plevris, Emilia; Hadjiyannis, Yannis; Perez, Miguel; Nolan, Eric; Kladney, Raleigh; Westendorp, Bart; de Bruin, Alain; Fernandez, Soledad; Rosol, Thomas J; Pohar, Kamal S; Pipas, James M; Leone, Gustavo

2017-03-01

Disruption of the retinoblastoma (RB) tumor suppressor pathway, either through genetic mutation of upstream regulatory components or mutation of RB1 itself, is believed to be a required event in cancer. However, genetic alterations in the RB-regulated E2F family of transcription factors are infrequent, casting doubt on a direct role for E2Fs in driving cancer. In this work, a mutation analysis of human cancer revealed subtle but impactful copy number gains in E2F1 and E2F3 in hepatocellular carcinoma (HCC). Using a series of loss- and gain-of-function alleles to dial E2F transcriptional output, we have shown that copy number gains in E2f1 or E2f3b resulted in dosage-dependent spontaneous HCC in mice without the involvement of additional organs. Conversely, germ-line loss of E2f1 or E2f3b, but not E2f3a, protected mice against HCC. Combinatorial mapping of chromatin occupancy and transcriptome profiling identified an E2F1- and E2F3B-driven transcriptional program that was associated with development and progression of HCC. These findings demonstrate a direct and cell-autonomous role for E2F activators in human cancer.

Fingermark visualisation on uncirculated £5 (Bank of England) polymer notes: Initial process comparison studies.

Science.gov (United States)

Downham, Rory P; Brewer, Eleigh R; King, Roberto S P; Luscombe, Aoife M; Sears, Vaughn G

2017-06-01

Experiments were conducted to investigate the effectiveness of a range of fingermark visualisation processes on brand new, uncirculated, £5 polymer banknotes (and their test note predecessors), as produced by the Bank of England (BoE). In the main study of this paper, a total of 14 individual processes were investigated on BoE £5 polymer banknotes, which included both 'Category A' processes (as recommended in the Home Office Fingermark Visualisation Manual) as well as recently developed processes, including fpNatural ® 2 powder (cuprorivaite) from Foster+Freeman and a vacuum metal deposition sequence that evaporates silver followed by zinc. Results from this preliminary investigation indicate that fpNatural ® 2, multimetal deposition, Wet Powder ™ Black, iron oxide powder suspension and black magnetic powder are the most effective processes on these uncirculated £5 BoE polymer banknotes, when viewed under "primary viewing" conditions (white light or fluorescence). Additional fingermarks were visualised on the polymer banknotes following the subsequent use of reflected infrared imaging and lifting techniques, and with the benefit of these techniques taken into consideration, the aforementioned processes remained amongst the most effective overall. This work provides initial insight into fingermark visualisation strategies for BoE £5 polymer banknotes, and the need for further studies in order to generate mature operational guidance is emphasised. Copyright © 2017 Elsevier B.V. All rights reserved.

Specific IgE for Fag e 3 Predicts Oral Buckwheat Food Challenge Test Results and Anaphylaxis: A Pilot Study.

Science.gov (United States)

Yanagida, Noriyuki; Sato, Sakura; Maruyama, Nobuyuki; Takahashi, Kyohei; Nagakura, Ken-Ichi; Ogura, Kiyotake; Asaumi, Tomoyuki; Ebisawa, Motohiro

2018-01-01

Buckwheat (BW) is the source of a life-threatening allergen. Fag e 3-specific serum IgE (sIgE) is more useful than BW-sIgE for diagnosis; however, it is unknown whether Fag e 3-sIgE can predict oral food challenge (OFC) results and anaphylaxis. This study aimed to clarify the efficacy of Fag e 3-sIgE in predicting OFC results and anaphylaxis. We conducted a retrospective review of BW- and Fag e 3-sIgE data obtained using the ImmunoCAP® assay system and fluorescent enzyme-linked immunosorbent assay from children who underwent OFC using 3,072 mg of BW protein between July 2006 and March 2014 at Sagamihara National Hospital, Kanagawa, Japan. We analyzed 60 patients aged 1.9-13.4 years (median 6.0 years); 20 (33%) showed objective symptoms upon BW OFC. The patients without symptoms had significantly lower Fag e 3-sIgE than those with non-anaphylactic (p tested factor that significantly predicted positive OFC results (odds ratio 8.93, 95% confidence interval 3.10-25.73, p < 0.001) and OFC-induced anaphylaxis (2.67, 1.12-6.35, p = 0.027). We suggest that a threshold Fag e 3-sIgE level of 18.0 kUE/L has 95% probability of provoking a positive reaction to BW. Fag e 3-sIgE predicted OFC results and OFC-induced anaphylaxis. We further emphasize paying careful attention to the risk of BW OFC-induced anaphylaxis. © 2018 The Author(s) Published by S. Karger AG, Basel.

E3D, 3-D Elastic Seismic Wave Propagation Code

International Nuclear Information System (INIS)

Larsen, S.; Harris, D.; Schultz, C.; Maddix, D.; Bakowsky, T.; Bent, L.

2004-01-01

1 - Description of program or function: E3D is capable of simulating seismic wave propagation in a 3D heterogeneous earth. Seismic waves are initiated by earthquake, explosive, and/or other sources. These waves propagate through a 3D geologic model, and are simulated as synthetic seismograms or other graphical output. 2 - Methods: The software simulates wave propagation by solving the elasto-dynamic formulation of the full wave equation on a staggered grid. The solution scheme is 4-order accurate in space, 2-order accurate in time

eLearning and eMaking: 3D Printing Blurring the Digital and the Physical

Science.gov (United States)

Loy, Jennifer

2014-01-01

This article considers the potential of 3D printing as an eLearning tool for design education and the role of eMaking in bringing together the virtual and the physical in the design studio. eLearning has matured from the basics of lecture capture into sophisticated, interactive learning activities for students. At the same time, laptops and…

Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins.

Directory of Open Access Journals (Sweden)

Hirotaka Takahashi

Full Text Available Ubiquitination is a key post-translational modification in the regulation of numerous biological processes in eukaryotes. The primary roles of ubiquitination are thought to be the triggering of protein degradation and the regulation of signal transduction. During protein ubiquitination, substrate specificity is mainly determined by E3 ubiquitin ligase (E3. Although more than 600 genes in the human genome encode E3, the E3s of many target proteins remain unidentified owing to E3 diversity and the instability of ubiquitinated proteins in cell. We demonstrate herein a novel biochemical analysis for the identification of E3s targeting specific proteins. Using wheat cell-free protein synthesis system, a protein array containing 227 human and 23 mouse recombinant E3s was synthesized. To establish the high-throughput binding assay using AlphaScreen technology, we selected MDM2 and p53 as the model combination of E3 and its target protein. The AlphaScreen assay specifically detected the binding of p53 and MDM2 in a crude translation mixture. Then, a comprehensive binding assay using the E3 protein array was performed. Eleven of the E3s showed high binding activity, including four previously reported E3s (e.g., MDM2, MDM4, and WWP1 targeting p53. This result demonstrated the reliability of the assay. Another interactors, RNF6 and DZIP3-which there have been no report to bind p53-were found to ubiquitinate p53 in vitro. Further analysis showed that RNF6 decreased the amount of p53 in H1299 cells in E3 activity-dependent manner. These results suggest the possibility that the RNF6 ubiquitinates and degrades p53 in cells. The novel in vitro screening system established herein is a powerful tool for finding novel E3s of a target protein.

Definition of invariants in 3-D application: study of the number of jets in e+e- events

International Nuclear Information System (INIS)

Proriol, J.

1993-01-01

In e + e - events, the jets have a spherical 3D symmetry. The usual method to tag the number of jets is the clusterization using classical algorithms. A set of invariants is defined for 3D objects with a spherical symmetry. These new invariants are used to study the number of jets in e + e - events. (K.A.) 5 refs.; 2 figs

Caracterização do gene vip3A e toxicidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja

Directory of Open Access Journals (Sweden)

Camila Soares Figueiredo

2013-09-01

Full Text Available O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda e da lagarta-da-soja (Anticarsia gemmatalis. O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+ e inserido em células de Escherichia coli BL21 (DE3. A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG, visualizada em SDS-PAGE e detectada por "Western blot". Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.

The translation initiation factor 3 subunit eIF3K interacts with PML and associates with PML nuclear bodies

Energy Technology Data Exchange (ETDEWEB)

Salsman, Jayme; Pinder, Jordan; Tse, Brenda [Department of Pathology, Dalhousie University, P.O. Box 15000, Halifax, Nova Scotia, Canada B3H 4R2 (Canada); Corkery, Dale [Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia (Canada); Dellaire, Graham, E-mail: dellaire@dal.ca [Department of Pathology, Dalhousie University, P.O. Box 15000, Halifax, Nova Scotia, Canada B3H 4R2 (Canada); Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia (Canada)

2013-10-15

The promyelocytic leukemia protein (PML) is a tumor suppressor protein that regulates a variety of important cellular processes, including gene expression, DNA repair and cell fate decisions. Integral to its function is the ability of PML to form nuclear bodies (NBs) that serve as hubs for the interaction and modification of over 90 cellular proteins. There are seven canonical isoforms of PML, which encode diverse C-termini generated by alternative pre-mRNA splicing. Recruitment of specific cellular proteins to PML NBs is mediated by protein–protein interactions with individual PML isoforms. Using a yeast two-hybrid screen employing peptide sequences unique to PML isoform I (PML-I), we identified an interaction with the eukaryotic initiation factor 3 subunit K (eIF3K), and in the process identified a novel eIF3K isoform, which we term eIF3K-2. We further demonstrate that eIF3K and PML interact both in vitro via pull-down assays, as well as in vivo within human cells by co-immunoprecipitation and co-immunofluorescence. In addition, eIF3K isoform 2 (eIF3K-2) colocalizes to PML bodies, particularly those enriched in PML-I, while eIF3K isoform 1 associates poorly with PML NBs. Thus, we report eIF3K as the first known subunit of the eIF3 translation pre-initiation complex to interact directly with the PML protein, and provide data implicating alternative splicing of both PML and eIF3K as a possible regulatory mechanism for eIF3K localization at PML NBs. - Highlights: • The PML-I C-terminus, encoded by exon 9, interacts with translation factor eIF3K. • We identify a novel eIF3K isoform that excludes exon 2 (eIF3K-2). • eIF3K-2 preferentially associates with PML bodies enriched in PML-I vs. PML-IV. • Alternative splicing of eIF3K regulates association with PML bodies.

Osteogenic gene expression of murine osteoblastic (MC3T3-E1) cells under cyclic tension

International Nuclear Information System (INIS)

Kao, C T; Chen, C C; Cheong, U-I; Liu, S L; Huang, T H

2014-01-01

Low-level laser therapy (LLLT) can promote cell proliferation. The remodeling ability of the tension side of orthodontic teeth affects post-orthodontic stability. The purpose of the present study was to investigate the osteogenic effects of LLLT on osteoblast-like cells treated with a simulated tension system that provides a mechanical tension regimen. Murine osteoblastic (MC3T3-E1) cells were cultured in a Flexcell strain unit with programmed loads of 12% elongation at a frequency of 0.5 Hz for 24 and 48 h. The cultured cells were treated with a low-level diode laser using powers of 5 J and 10 J. The proliferation of MC3T3-E1 cells was determined using the Alamar Blue assay. The expression of osteogenic genes (type I collagen (Col-1), osteopontin (OPN), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), bone morphologic protein (BMP-2), and bone morphologic protein (BMP-4)) in MC3T3-E1 cells was analyzed using reverse transcription polymerase chain reaction (RT-PCR). The data were analyzed using one-way analysis of variance. The proliferation rate of tension-cultured MC3T3-E1 cells under 5 J and 10 J LLLT increased compared with that of the control group (p < 0.05). Prominent mineralization of the MC3T3-E1 cells was visible using a von Kossa stain in the 5 J LLLT group. Osteogenic genes (Col-1, OC, OPG and BMP-2) were significantly expressed in the MC3T3-E1 cells treated with 5 J and 10 J LLLT (p < 0.05). LLLT in tension-cultured MC3T3-E1 cells showed synergistic osteogenic effects, including increases in cell proliferation and Col-1, OPN, OC, OPG and BMP-2 gene expression. LLLT might be beneficial for bone remodeling on the tension side of orthodontics. (paper)

The EISCAT_3D Project in Norway: E3DN

Science.gov (United States)

La Hoz, C.; Oksavik, K.

2013-12-01

EISCAT_3D (E3D) is a project to build the next generation of incoherent scatter radars endowed with 3-dimensional scalar and vector capabilities that will replace the current EISCAT radars in Northern Scandinavia. One active (transmitting) site in Norway and four passive (receiving) sites in the Nordic countries will provide 3-D vector imaging capabilities by rapid scanning and multi-beam forming. The unprecedented flexibility of the solid-state transmitter with high duty-cycle, arbitrary wave-forming and polarisation and its pulsed power of 10 MW will provide unrivalled experimental capabilities to investigate the highly non-stationary and non-homogeneous state of the polar upper atmosphere. Aperture Synthesis Imaging Radar (ASIR) will to endow E3D with imaging capabilities in 3-dimensions that includes sub-beam resolution. Complemented by pulse compression, it will provide 3-dimensional images of certain types of incoherent scatter radar targets resolved to about 100 metres at 100 km range, depending on the signal-to-noise ratio. The Norwegian scientific programme is inspired by the pioneer polar scientist Kristian Birkeland (picture) and includes pressing questions on polar upper atmospheric research, among others: (Q1) How to proceed beyond the present simplistic, static, stationary and homogeneous analysis of upper atmospheric and ionospheric processes? (Q2) How does space weather affect ionospheric processes and how to support modelling and space weather services? (Q3) How to advance fundamental plasma physics by employing the ionosphere as a natural plasma physics laboratory? (Q4) How does the influx of extraterrestrial material interact with the upper atmosphere and where does the material originate from? (Q5) How does solar activity couple from geospace into the lower atmosphere and climate system, and does this energy change the wave forcing of geospace from below? Kristian Birkeland, Norwegian scientist and pioneer in polar and auroral research.

A Sentry on the Universe

Science.gov (United States)

Dixon, Robert

This chapter introduces the Argus radio telescope concept, a radically new approach to radio telescope design (not to be confused with The SETI League's Project Argus, a linked global array of fairly conventional radio telescopes). It represents a complete departure from designs that have been used ever since the invention of the first telescope by Galileo in 1609. It starts over from the beginning, and in doing so overcomes the legacy of Galileo. Argus can do things that are amazing and even hard to believe, when viewed in the context of previous telescopes. Here are some examples.

Sentry Assurance: Bringing Outsiders "In."

Science.gov (United States)

Lease, Judy E.

1998-01-01

Presents a case study for use in business communication classes to help students understand and learn both the context and the strategies for communication with business and management. Deals with multiple problems caused by the rapid expansion of an insurance brokerage, including gender issues, clarification of responsibilities and roles, and…

SyPRID sampler: A large-volume, high-resolution, autonomous, deep-ocean precision plankton sampling system

Science.gov (United States)

Billings, Andrew; Kaiser, Carl; Young, Craig M.; Hiebert, Laurel S.; Cole, Eli; Wagner, Jamie K. S.; Van Dover, Cindy Lee

2017-03-01

The current standard for large-volume (thousands of cubic meters) zooplankton sampling in the deep sea is the MOCNESS, a system of multiple opening-closing nets, typically lowered to within 50 m of the seabed and towed obliquely to the surface to obtain low-spatial-resolution samples that integrate across 10 s of meters of water depth. The SyPRID (Sentry Precision Robotic Impeller Driven) sampler is an innovative, deep-rated (6000 m) plankton sampler that partners with the Sentry Autonomous Underwater Vehicle (AUV) to obtain paired, large-volume plankton samples at specified depths and survey lines to within 1.5 m of the seabed and with simultaneous collection of sensor data. SyPRID uses a perforated Ultra-High-Molecular-Weight (UHMW) plastic tube to support a fine mesh net within an outer carbon composite tube (tube-within-a-tube design), with an axial flow pump located aft of the capture filter. The pump facilitates flow through the system and reduces or possibly eliminates the bow wave at the mouth opening. The cod end, a hollow truncated cone, is also made of UHMW plastic and includes a collection volume designed to provide an area where zooplankton can collect, out of the high flow region. SyPRID attaches as a saddle-pack to the Sentry vehicle. Sentry itself is configured with a flight control system that enables autonomous survey paths to low altitudes. In its verification deployment at the Blake Ridge Seep (2160 m) on the US Atlantic Margin, SyPRID was operated for 6 h at an altitude of 5 m. It recovered plankton samples, including delicate living larvae, from the near-bottom stratum that is seldom sampled by a typical MOCNESS tow. The prototype SyPRID and its next generations will enable studies of plankton or other particulate distributions associated with localized physico-chemical strata in the water column or above patchy habitats on the seafloor.

Determination of the proton spectral function of 3He with the (e,e'p) reaction

International Nuclear Information System (INIS)

Jans, E.

1982-01-01

Cross sections of the 3 He(e,e'p) reaction have been measured with the 600 MeV linear accelerator at Saclay and the two-spectrometer set-up of the experimental hall HE1. The data cover the following region for the missing energy Esub(m) and the proton recoil momentum k: 0 3 He target are described in chapter IV. The experimental procedure, the data analysis, and the corrections which have been applied to the data, are discussed in chapter V. The results of the experiment are presented in chapter VI. The (e,e'p) reaction has proven its capabilities in obtaining detailed information on nuclear structure properties as nucleon momentum density distributions and separation energies, although corrections due to FSI and MEC have to be considered. In the case of 3 He, the results can be compared to predictions of various calculational techniques, using realistic NN-potentials and thus serve as a test for NN-potential models. (Auth.)

Antitumor properties of (5E,7E) analogs of vitamin D3.

Science.gov (United States)

Filip, B; Milczarek, M; Wietrzyk, J; Chodyński, M; Kutner, A

2010-07-01

Geometric isomers (5E,7E) of major active metabolites of vitamin D3 [1alpha,25(OH)2D3 and (24R)-1,24(OH)2D3] were synthesized by a new convenient procedure. Vitamin D triene system of the metabolites was first derivatized as a Diels-Alder adduct. Removal of the triene protecting group, in a key synthetic step, yielded the title compounds PRI-2208 and PRI-2209, respectively. The analogs were examined for their antiproliferative activity in vitro against human breast cancer cells (MCF-7) and promyelocytic leukemia (HL-60) cells. The activity was compared with one of the parent compounds. Both analogs examined revealed similar or higher antiproliferative activity compared to 1alpha,25(OH)2D3 or to (24R)-1,24(OH)2D3. The studies of calcemic activity in vivo showed that analogs PRI-2208 and PRI-2209 did not influence the serum calcium level in doses, in which 1alpha,25(OH)2D3 or (24R)-1,24(OH)2D3 significantly increased this level. The antitumor activity of these analogs in the LLC mice tumor model was studied. Analog PRI-2208 was found to be more active in inhibiting LLC tumor growth than 1alpha,25(OH)2D3, as well as than PRI-2191 and PRI-2209. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Mechanics and cooling of the Mu3e detector

Energy Technology Data Exchange (ETDEWEB)

Austermuehl, Rene Philipp; Henkelmann, Lars; Herkert, Adrian; Ng, Yanwing [Physikalisches Institut, Uni Heidelberg (Germany); Collaboration: Mu3e-Collaboration

2016-07-01

The Mu3e experiment will search for the charged lepton flavour violating decay μ{sup +} → e{sup +}e{sup -}e{sup +}, which is suppressed to unobservable levels in the Standard Model. A signal would be a clear sign of new physics. The aim is to reach a sensitivity for the branching ratio of 10{sup -16}, which requires high momentum resolution. Since the muons will decay at rest on target, the energy of the decay electrons will be E≤53 MeV. In this energy regime the momentum resolution is limited by multiple scattering in the detector material. Therefore, the Mu3e detector, including support structure and services, has to consist of as little material as possible in the active region. The main component of the detector is a pixel tracker consisting of four barrels of high voltage monolithic active pixel sensors (HV-MAPS) that can be thinned to 50 μm. A power consumption of 400 mW/cm{sup 2} is expected. To keep the material budget low, it is planned to cool with gaseous helium. In this talk results of computational fluid dynamics simulations of the Mu3e cooling system and experimental tests concerning the detector's stability against flow-induced vibrations are presented.

Electronic and structural properties of B i2S e3:Cu

Science.gov (United States)

Sobczak, Kamil; Strak, Pawel; Kempisty, Pawel; Wolos, Agnieszka; Hruban, Andrzej; Materna, Andrzej; Borysiuk, Jolanta

2018-04-01

Electronic and structural properties of B i2S e3 and its extension to copper doped B i2S e3:Cu were studied using combined ab initio simulations and transmission electron microscopy based techniques, including electron energy loss spectroscopy, energy filtered transmission electron microscopy, and energy dispersive x-ray spectroscopy. The stability of the mixed phases was investigated for substitutional and intercalation changes of basic B i2S e3 structure. Four systems were compared: B i2S e3 , structures obtaining by Cu intercalation of the van der Waals gap, by substitution of Bi by Cu in quintuple layers, and C u2Se . The structures were identified and their electronic properties were obtained. Transmission electron microscopy measurements of B i2S e3 and the B i2S e3:Cu system identified the first structure as uniform and the second as composite, consisting of a nonuniform lower-Cu-content matrix and randomly distributed high-Cu-concentration precipitates. Critical comparison of the ab initio and experimental data identified the matrix as having a B i2S e3 dominant part with randomly distributed Cu-intercalated regions having 1Cu-B i2S e3 structure. The precipitates were determined to have 3Cu-B i2S e3 structure.

The structures of colour string for e+e- → qq-barg and υ → 3g

International Nuclear Information System (INIS)

Tian Lili; Xie Qubing; Si Zongguo

1993-01-01

In Lund model, the explanation of e + e - → qq-barg → 3 jets and υ → 3g → h's is based on applying Lund string fragmentation model to their assumed structures of colour string for qq-barg and 3g systems. In this paper, starting from the colour wave functions of qq-barg and 3 g systems, we study these colour string structures by QCD directly. The results reveal the reasonableness and accuracy of Lund string pictures

Jefferson Lab E89-044 experiment: study of the quasi-elastic He3(e,e'p)d reaction in parallel kinematics

International Nuclear Information System (INIS)

Penel-Nottaris, E.

2004-07-01

The Jefferson Lab Hall A E89-044 experiment has measured the He 3 (e,e'p) reaction cross-sections. The extraction of the longitudinal and transverse response functions for the two-body break-up He 3 (e,e'p)d reaction in parallel kinematics allows the study of the bound proton electromagnetic properties inside the He 3 nucleus and the involved nuclear mechanisms beyond plane waves approximations, for missing momenta of 0 and +- 300 MeV/c and transferred momenta from 0.8 to 4.1 GeV 2 . Preliminary cross-sections have been obtained after calibration of the experimental setup by fitting theoretical models averaged over the experimental phase-space using a Monte-Carlo simulation. The 8% systematic error on cross-sections is linked mainly to the absolute normalization of the target density: the elastic scattering data analysis will allow to reduce this error. The preliminary results show some disagreement with theoretical predictions for the forward angles kinematics around 0 MeV/c missing momenta and sensitivity to final state interactions and He 3 waves functions for missing momenta of 300 MeV/c. The longitudinal and transverse separation should constraint theoretical models more strongly. (author)

Lysophosphatidic acid induces chemotaxis in MC3T3-E1 osteoblastic cells

Energy Technology Data Exchange (ETDEWEB)

Masiello, Lisa M.; Fotos, Joseph S.; Galileo, Deni S.; Karin, Norm J.

2006-07-01

Lysophosphatidic acid (LPA) is a bioactive lipid that has pleiotropic effects on a variety of cell types and enhances the migration of endothelial and cancer cells, but it is not known if this lipid can alter osteoblast motility. We performed transwell migration assays using MC3T3-E1 osteoblastic cells and found LPA to be a potent chemotactic agent. Quantitative time-lapse video analysis of osteoblast migration after wounds were introduced into cell monolayers indicated that LPA stimulated both migration velocity and the average migration distance per cell. LPA also elicited substantial changes in cell shape and actin cytoskeletal structure; lipid-treated cells contained fewer stress fibers and displayed long membrane processes that were enriched in F-actin. Quantitative RT-PCR analysis showed that MC3T3-E1 cells express all four known LPA-specific G protein-coupled receptors (LPA1-LPA4) with a relative mRNA abundance of LPA1 > LPA4 > LPA2 >> LPA3. LPA-induced changes in osteoblast motility and morphology were antagonized by both pertussis toxin and Ki16425, a subtype-specific blocker of LPA1 and LPA3 receptor function. Cell migration in many cell types is linked to changes in intracellular Ca2+. Ki16425 also inhibited LPA-induced Ca2+ signaling in a dose-dependent manner, suggesting a link between LPA-induced Ca2+ transients and osteoblast chemotaxis. Our data show that LPA stimulates MC3T3-E1 osteoblast motility via a mechanism that is linked primarily to the G protein-coupled receptor LPA1.

Radiative K{sub e3} decays revisited

Energy Technology Data Exchange (ETDEWEB)

Gasser, J. [Universitaet Bern, Institut fuer Theoretische Physik, Bern (Switzerland); Kubis, B. [Universitaet Bern, Institut fuer Theoretische Physik, Bern (Switzerland); Universitaet Bonn, Helmholtz-Institut fuer Strahlen- und Kernphysik, Bonn (Germany); Paver, N. [Universita degli Studi di Trieste, Dipartimento di Fisica Teorica, Trieste (Italy); INFN-Trieste, Trieste (Italy); Verbeni, M. [Universidad de Granada, Departamento de Fisica Teorica y del Cosmos, Granada (Spain)

2005-03-01

Motivated by recent experimental results and ongoing measurements, we review the chiral perturbation theory prediction for K{sub L}{yields}{pi}{sup -+}e{sup {+-}}{nu}{sub e}{gamma} decays. Special emphasis is given to the stability of the inner bremsstrahlung-dominated relative branching ratio versus the K{sub e3} form factors, and on the separation of the structure-dependent amplitude in differential distributions over the phase space. For the structure-dependent terms, an assessment of the order p{sup 6} corrections is given, in particular, a full next-to-leading order calculation of the axial component is performed. The experimental analysis of the photon energy spectrum is discussed, and other potentially useful distributions are introduced. (orig.)

Deviations between experimental and theoretical results in Ar (e, 3e) double ionization

International Nuclear Information System (INIS)

Jia, C C; Lahmam-Bennani, A; Cappello, C Dal; Duguet, A; Avaldi, L

2003-01-01

The coplanar (e, 3e) relative cross sections for double ionization of argon have been measured at an electron impact energy of E 0 = 561.4 eV and under equal energy sharing among the two 'ejected' electrons, E b = E c = 9 eV. The scattering angle is fixed to θ a = 1.5 degrees, corresponding to a momentum transfer K=0.4 au to the target. The experimental results have been compared with calculations in the first Born approximation, which include only first-order processes in the target-projectile interaction. The comparison shows severe deviations between the experimental and theoretical results. These deviations are much larger than the ones previously observed in helium under comparable kinematics. To fill this gap between theory and experiment, a decisive improvement in the theory is needed. This can be achieved by improving the first-order calculations and by including higher-order processes such as the two-step mechanism, or even new mechanisms, for instance the simultaneous ejection of the pair of target electrons. (letter to the editor)

ClC-3 Promotes Osteogenic Differentiation in MC3T3-E1 Cell After Dynamic Compression.

Science.gov (United States)

Wang, Dawei; Wang, Hao; Gao, Feng; Wang, Kun; Dong, Fusheng

2017-06-01

ClC-3 chloride channel has been proved to have a relationship with the expression of osteogenic markers during osteogenesis, persistent static compression can upregulate the expression of ClC-3 and regulate osteodifferentiation in osteoblasts. However, there was no study about the relationship between the expression of ClC-3 and osteodifferentiation after dynamic compression. In this study, we applied dynamic compression on MC3T3-E1 cells to detect the expression of ClC-3, runt-related transcription factor 2 (Runx2), bone morphogenic protein-2 (BMP-2), osteopontin (OPN), nuclear-associated antigen Ki67 (Ki67), and proliferating cell nuclear antigen (PCNA) in biopress system, then we investigated the expression of these genes after dynamic compression with Chlorotoxin (specific ClC-3 chloride channel inhibitor) added. Under transmission electron microscopy, there were more cell surface protrusions, rough surfaced endoplasmic reticulum, mitochondria, Golgi apparatus, abundant glycogen, and lysosomes scattered in the cytoplasm in MC3T3-E1 cells after dynamic compression. The nucleolus was more obvious. We found that ClC-3 was significantly up-regulated after dynamic compression. The compressive force also up-regulated Runx2, BMP-2, and OPN after dynamic compression for 2, 4 and 8 h. The proliferation gene Ki67 and PCNA did not show significantly change after dynamic compression for 8 h. Chlorotoxin did not change the expression of ClC-3 but reduced the expression of Runx2, BMP-2, and OPN after dynamic compression compared with the group without Cltx added. The data from the current study suggested that ClC-3 may promotes osteogenic differentiation in MC3T3-E1 cell after dynamic compression. J. Cell. Biochem. 118: 1606-1613, 2017. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

76 FR 71472 - Airworthiness Directives; The Boeing Company Airplanes

Science.gov (United States)

2011-11-18

... seal contacts the 12-o'clock engine strut, and for correct stiffness and vent holes, and doing... January 3, 2012. ADDRESSES: You may send comments by any of the following methods: Federal eRulemaking... the seal is attributed to insufficient seal stiffness and/or missing vent holes. If a damaged seal...

Is e+e- pair emission important in the determination of the 3He+4He S factor?

Science.gov (United States)

Snover, K. A.; Hurd, A. E.

2003-05-01

We show that the cross section for direct E0 pair emission is related to the cross section for direct E2 photon emission, and is a negligible contribution to the total capture cross section for 3He+4He→7Be. E0 resonance emission, E1 pair emission, and internal conversion are also negligible. Thus there cannot be significant contributions to the 3He+4He→7Be capture cross section at low energies from electromagnetic emission processes other than single photon emission.

3DHOP: una piattaforma flessibile per la pubblicazione e visualizzazione su Web dei risultati di digitalizzazioni 3D

Directory of Open Access Journals (Sweden)

Marco Potenziani

2016-04-01

Full Text Available 3D Heritage Online Presenter (3DHOP è una piattaforma per integrare all’interno di pagine internet modelli 3D ad alta risoluzione, quali ad esempio quelli ottenuti per scansione 3D, progettata per far fronte alle specifiche esigenze del settore Cultural Heritage (CH. 3DHOP gestisce la complessità dei dati in ingresso mediante un motore di rappresentazione e rendering multirisoluzione, pertanto, è in grado di trasmettere in modo progressivo ed efficiente anche modelli 3D di grandi dimensioni. 3DHOP fornisce una serie di template pronti all’uso per la presentazione di modelli CH, collegando la visualizzazione 3D con il resto della pagina Web e rendendo possibile la creazione di presentazioni integrate (3D ed altri contenuti multimediali. Nella sua progettazione e sviluppo abbiamo posto particolare attenzione alla facilità d'uso ed alle prestazioni della piattaforma, che dunque è caratterizzata da una curva di apprendimento sostenibile e da performance ottimali nella visualizzazione di modelli ad alta risoluzione. Grazie alla sua natura modulare e una configurazione dichiarativa, imparare ad usare, configurare e personalizzare a diversi livelli 3DHOP è alla portata anche di personale non esperto sui temi della grafica 3D. Questo permette ad utenti con esperienza diversa (utilizzatori finali, sviluppatori di pagine Web, programmatori un diverso accesso al sistema. 3DHOP è scritto principalmente in JavaScript ed è basato sulla libreria SpiderGL, che a sua volta poggia sulla componente WebGL di HTML5. Ciò permette di gestire il rendering di modelli 3D su tutti i più comuni browser Web e senza richiedere l’uso di plugin. In questo articolo presentiamo le capacità e le caratteristiche della piattaforma 3DHOP, utilizzando diversi esempi pratici d’uso.

Expectation values of the e+He(3Se) system

International Nuclear Information System (INIS)

Mitroy, J.

2005-01-01

Close to converged energies and expectation values for e + He( 3 S e ) are computed using a ground state wave function consisting of 1500 explicitly correlated Gaussians. The best estimate of the e + He( 3 S e ) energy was -2.250 595 08 hartree, which has a binding energy of 0.000 595 08 hartree against dissociation into Ps+He + . The 2γ annihilation rate for the spin doublet state was 5.713x10 9 s -1 . The estimated annihilation rate with the core He + electron was 2.506x10 6 s -1 . The derived enhancement factor for annihilation with the He + (1s) core was 2.29, just over 10% smaller than the enhancement factor derived from analyses of annihilation during e + -He + scattering. The diffuse nature of the wave function, with well separated He + and Ps subsystems, is demonstarted to be on the threshold of satisfying the formal criteria that define a quantum halo state

Rate Coefficient Measurements and Theoretical Analysis of the OH + ( E) CF3CH=CHCF3 Reaction.

Science.gov (United States)

Baasandorj, Munkhbayar; Marshall, Paul; Waterland, Robert L; Ravishankara, Akkihebbal R; Burkholder, James B

2018-04-25

Rate coefficients, k, for the gas-phase reaction of the OH radical with (E) CF3CH=CHCF3 ((E)-1,1,14,4,4-hexafluoro-2-butene, HFO-1336mzz(E)) were measured over a range of temperature (211-374 K) and bath gas pressure (20-300 Torr; He, N2) using a pulsed laser photolysis-laser induced fluorescence (PLP-LIF) technique. k1(T) was independent of pressure over this range of conditions with k1(296 K) = (1.31 ± 0.15) × 10 13 cm3 molecule 1 s 1 and k1(T) = (6.94 ± 0.80) × 10 13 exp[ (496 ± 10)/T] cm3 molecule 1 s 1, where the uncertainties are 2 and the pre-exponential term includes estimated systematic error. Rate coefficients for the OD reaction were also determined over a range of temperature (262-374 K) at 100 Torr (He). The OD rate coefficients were ~15% greater than the OH values and showed similar temperature dependent behavior with k2(T) = (7.52 ± 0.44) × 10 13 exp[ (476 ± 20)/T] and k2(296 K) = (1.53 ± 0.15) × 10 13 cm3 molecule 1 s 1. The rate coefficients for reaction 1 were also measured using a relative rate technique between 296 and 375 K with k1(296 K) measured to be (1.22 ± 0.1) × 10 13 cm3 molecule 1 s 1 in agreement with the PLP-LIF results. In addition, the 296 K rate coefficient for the O3 + (E) CF3CH=CHCF3 reaction was determined to be reaction and the significant decrease in OH reactivity compared to the (Z) CF3CH=CHCF3 stereoisomer reaction. The estimated atmospheric lifetime of (E) CF3CH=CHCF3, due to loss by reaction with OH, is estimated to be ~90 days, while the actual lifetime will depend on the location and season of its emission. Infrared absorption spectra of (E) CF3CH=CHCF3 were measured and used to estimate the 100-year time horizon global warming potentials (GWP) of 32 (atmospherically well-mixed) and 14 (lifetime-adjusted).

The clinical value of HPV E6/E7 and STAT3 mRNA detection in cervical cancer screening.

Science.gov (United States)

Fan, Yibing; Shen, Zongji

2018-02-11

To explore the value of human papillomavirus (HPV) E6/E7 and signal transducer and activator of transcription 3 (STAT3) mRNA detection in the screening of cervical lesions. 192 patients with abnormal ThinPrep cytology test (TCT) results and/or high-risk HPV infection were screened to identify possible cervical lesions in cases. Diagnoses were confirmed by histopathology. Fluorescence in situ hybridization (FISH) was performed to detect and qualify the mRNAs of HPV E6/E7, STAT3, and Survivin in cervical exfoliated cells. In addition, the performance of separate and combined mRNA detection methods were compared with TCT, HR-HPV DNA schemes respectively. 1. Compared with HPVE6/E7 and STAT3 mRNA methods, Survivin mRNA assay had poor specificity (Sp), Youden index (YI) and concordance rate. 2. HPV E6/E7, STAT3, and STAT3 + HR-HPV methods had the best Sp, concordance rate and positive predictive value (PPV) for cervical lesions screening and atypical squamous cells of undetermined significance (ASCUS) triage. For screening of high grade squamous intraepithelial lesions or greater (HSILs+), no difference was observed in the Se of mRNA detection methods in comparison with that of TCT, HR-HPV and TCT + HR-HPV, whereas the false positive rate (FPR) decreased by 41.48%/55.99%/17.19% and the colposcopy referral rate reduced by about 20.00%/25.00%/11.17%. For triage of women with ASCUS, no difference was observed in the Se of mRNA detection methods as compared to that of HR-HPV (χ 2  = 1.05, P > 0.75), while the FPR decreased by 45.83%/37.50%/41.66% and the colposcopy referral rate reduced by 32.42%/22.60%/25.28%, respectively. The Se, YI, and PPV of the combined methods increased in comparison to each method alone. 3. Compared with the TCT + HR-HPV method, HPV E6/E7 + STAT3 method had perfect Sp (95.92%) and PPV (95.40%) for screening HSILs+, the FPR and colposcopy referral rate decreased by 31.06% and 22.48% respectively. 1. The expression of HPV E6/E

Novel (1E,3E,5E-1,6-bis(Substituted phenylhexa-1,3,5-triene Analogs Inhibit Melanogenesis in B16F10 Cells and Zebrafish

Directory of Open Access Journals (Sweden)

Jisun Oh

2018-04-01

Full Text Available The present study aimed to evaluate the anti-melanogenic activity of 1,6-diphenyl-1,3,5-hexatriene and its derivatives in B16F10 murine melanoma cells and zebrafish embryos. Twenty five (1E,3E,5E-1,6-bis(substituted phenylhexa-1,3,5-triene analogs were synthesized and their non-cytotoxic effects were predictively analyzed using three-dimensional quantitative structure-activity relationship approach. Inhibitory activities of these synthetic compounds against melanin synthesis were determined by evaluating melanin content and melanogenic regulatory enzyme expression in B16F10 cells. The anti-melanogenic activity was verified by observing body pigmentation in zebrafishes treated with these compounds. Compound #2, #4, and #6 effectively decreased melanogenesis induced by α-melanocyte-stimulating hormone. In particular, compound #2 remarkably lowered the mRNA and protein expression levels of microphthalmia-associated transcription factor (MITF, tyrosinase (TYR, tyrosinase-related protein 1 (TYRP1, and TYRP2 in B16F10 cells and substantially reduced skin pigmentation in the developed larvae of zebrafish. These findings suggest that compound #2 may be used as an anti-melanogenic agent for cosmetic purpose.

Research study on antiskid braking systems for the space shuttle

Science.gov (United States)

Auselmi, J. A.; Weinberg, L. W.; Yurczyk, R. F.; Nelson, W. G.

1973-01-01

A research project to investigate antiskid braking systems for the space shuttle vehicle was conducted. System from the Concorde, Boeing 747, Boeing 737, and Lockheed L-1011 were investigated. The characteristics of the Boeing 737 system which caused it to be selected are described. Other subjects which were investigated are: (1) trade studies of brake control concepts, (2) redundancy requirements trade study, (3) laboratory evaluation of antiskid systems, and (4) space shuttle hardware criteria.

A 3D Virtual Space for the E-Commerce Strategy Model

OpenAIRE

Gong Cheng; Changrui Yu; Kecheng Liu

2014-01-01

In recent years, innovative applications of electronic commerce (e-commerce) are facing the challenges of mobile commerce (m-commerce) and ubiquitous commerce (u-commerce). To address these challenges and improve the competitiveness of e-commerce, the three-dimensional e-commerce (3DEC) theory is proposed and a 3DEC business strategy model is built up in this study. The new conceptual 3DEC model incorporates the industrial environment analysis, SWOT analysis, and business model canvas (BMC). ...

Laser scanner 3D terrestri e mobile

Directory of Open Access Journals (Sweden)

Mario Ciamba

2013-08-01

Full Text Available Recentemente si è svolto a Roma un evento dimostrativo per informare, professionisti e ricercatori del settore inerente il rilievo strumentale, sulle recenti innovazioni che riguardano i laser scanner 3d. Il mercato della strumentazione dedicata al rilevamento architettonico e dell'ambiente, offre molte possibilità di scelta. Oggi i principali marchi producono strumenti sempre più efficienti ed ideati per ambiti di applicazione specifici, permettendo ai professionisti, la giusta scelta in termini di prestazioni ed economia. A demonstration event was recently held in Rome with the aim to inform professionals and researchers on recent innovations on instrumental survey related to the 3d laser scanner. The market of instrumentation for architectural survey offers many possibilitiesof choice. Today the major brands produce instruments that are more efficient and designed for specific areas of application, allowing the right choice in terms of performance and economy.

Laser scanner 3D terrestri e mobile

Directory of Open Access Journals (Sweden)

Mario Ciamba

2013-08-01

Full Text Available Recentemente si è svolto a Roma un evento dimostrativo per informare, professionisti e ricercatori del settore inerente il rilievo strumentale, sulle recenti innovazioni che riguardano i laser scanner 3d. Il mercato della strumentazione dedicata al rilevamento architettonico e dell'ambiente, offre molte possibilità di scelta. Oggi i principali marchi producono strumenti sempre più efficienti ed ideati per ambiti di applicazione specifici, permettendo ai professionisti, la giusta scelta in termini di prestazioni ed economia.A demonstration event was recently held in Rome with the aim to inform professionals and researchers on recent innovations on instrumental survey related to the 3d laser scanner. The market of instrumentation for architectural survey offers many possibilitiesof choice. Today the major brands produce instruments that are more efficient and designed for specific areas of application, allowing the right choice in terms of performance and economy.

Characterisation of 5-HT3C, 5-HT3D and 5-HT3E receptor subunits: evolution, distribution and function.

Science.gov (United States)

Holbrook, Joanna D; Gill, Catherine H; Zebda, Noureddine; Spencer, Jon P; Leyland, Rebecca; Rance, Kim H; Trinh, Han; Balmer, Gemma; Kelly, Fiona M; Yusaf, Shahnaz P; Courtenay, Nicola; Luck, Jane; Rhodes, Andrew; Modha, Sundip; Moore, Stephen E; Sanger, Gareth J; Gunthorpe, Martin J

2009-01-01

The 5-HT(3) receptor is a member of the 'Cys-loop' family of ligand-gated ion channels that mediate fast excitatory and inhibitory transmission in the nervous system. Current evidence points towards native 5-HT(3) receptors originating from homomeric assemblies of 5-HT(3A) or heteromeric assembly of 5-HT(3A) and 5-HT(3B). Novel genes encoding 5-HT(3C), 5-HT(3D), and 5-HT(3E) have recently been described but the functional importance of these proteins is unknown. In the present study, in silico analysis (confirmed by partial cloning) indicated that 5-HT(3C), 5-HT(3D), and 5-HT(3E) are not human-specific as previously reported: they are conserved in multiple mammalian species but are absent in rodents. Expression profiles of the novel human genes indicated high levels in the gastrointestinal tract but also in the brain, Dorsal Root Ganglion (DRG) and other tissues. Following the demonstration that these subunits are expressed at the cell membrane, the functional properties of the recombinant human subunits were investigated using patch clamp electrophysiology. 5-HT(3C), 5-HT(3D), and 5-HT(3E) were all non-functional when expressed alone. Co-transfection studies to determine potential novel heteromeric receptor interactions with 5-HT(3A) demonstrated that the expression or function of the receptor was modified by 5-HT(3C) and 5-HT(3E), but not 5-HT(3D). The lack of distinct effects on current rectification, kinetics or pharmacology of 5-HT(3A) receptors does not however provide unequivocal evidence to support a direct contribution of 5-HT(3C) or 5-HT(3E) to the lining of the ion channel pore of novel heteromeric receptors. The functional and pharmacological contributions of these novel subunits to human biology and diseases such as irritable bowel syndrome for which 5-HT(3) receptor antagonists have major clinical usage, therefore remains to be fully determined.

Particle Trajectory and Icing Analysis of the E(sup 3) Turbofan Engine Using LEWICE3D Version 3

Science.gov (United States)

Bidwell, Colin S.

2011-01-01

Particle trajectory and ice shape calculations were made for the Energy Efficient Engine (E(sup 3)) using the LEWICE3D Version 3 software. The particle trajectory and icing computations were performed using the new "block-to-block" collection efficiency method which has been incorporated into the LEWICE3D Version 3 software. The E(sup 3) was developed by NASA and GE in the early 1980 s as a technology demonstrator and is representative of a modern high bypass turbofan engine. The E(sup 3) flow field was calculated using the NASA Glenn ADPAC turbomachinery flow solver. Computations were performed for the low pressure compressor of the E(sup 3) for a Mach 0.8 cruise condition at 11,887 m assuming a standard warm day for three drop sizes and two drop distributions typically used in aircraft design and certification. Particle trajectory computations were made for water drop sizes of 5, 20, and 100 microns. Particle trajectory and ice shape predictions were made for a 20 micron Langmuir-D distribution and for a 92 mm Super-cooled Large Droplet (SLD) distribution with and without splashing effects for a Liquid Water Content (LWC) of 0.3 g/cu m and an icing time of 30 min. The E3 fan and spinner combination proved to be an effective ice removal mechanism as they removed greater than 36 percent of the mass entering the inlet for the icing cases. The maximum free stream catch fraction for the fan and spinner combination was 0.60 while that on the elements downstream of the fan was 0.03. The non-splashing trajectory and collection efficiency results showed that as drop size increased impingement rates increased on the spinner and fan leaving less mass to impinge on downstream components. The SLD splashing case yielded more mass downstream of the fan than the SLD non-splashing case due to mass being splashed from the upstream inlet lip, spinner and fan components. The ice shapes generated downstream of the fan were either small or nonexistent due to the small available mass

Trajectory Determination for Chang 'e-3 Probe Soft-landing

Science.gov (United States)

Yezhi, S.; Huang, Y.

2017-12-01

On December 2, 2013, The Chang 'e-3 (ce-3) probe was successfully launched from a long march-3b carrier rocket at Xichang satellite launch center. After more than five days of flying, the probe was captured by the moon to 100 km by 100 km. The orbit maneuvered to 15 km by 100 km 4 days later. Finally, at 21:12 Beijing time on December 14, 2013, it landed at the junction of the Sinus Iridum and Mare Imbrium. In the ce-3 project, the combined test mode of the radio ranging measurement and very long baseline interferometry (VLBI) was used. The soft-landing was carried out in ce-3 mission for the sampling .The paper presents a new method of trajectory determination for soft landing and sampling returning for lunar probe by B spline approximation. By simulation and data processing of Chang'E-3(CE-3), it could be assumed that the accuracy of trajectory determination of soft landing is less than 100 meters in CE-3. It appears that the difference between the endpoint of trajectory and the location from image processed by NASA'S LRO is less than 50m .It confirms the method of soft landing trajectory determination provided by the paper is effective. The paper analyzes the dynamics and control characteristics of the sampling returning, provides the preliminary feasible trajectory determination method for soft landing and sampling return of Chang'E-5 (CE - 5).

Fractal dimensions from a 3-dimensional intermittency analysis in e+e- annihilation

International Nuclear Information System (INIS)

Behrend, H.J.; Criegee, L.; Field, J.H.; Franke, G.; Jung, H.; Meyer, J.; Podobrin, O.; Schroeder, V.; Winter, G.G.; Bussey, P.J.; Campbell, A.J.; Hendry, D.; Lumsdon, S.J.; Skillicorn, I.O.; Ahme, J.; Blobel, V.; Feindt, M.; Fenner, H.; Harjes, J.; Koehne, J.H.; Peters, J.H.; Spitzer, H.; Weihrich, T.; Boer, W. de; Buschhorn, G.; Grindhammer, G.; Gunderson, B.; Kiesling, C.; Kotthaus, R.; Kroha, H.; Lueers, D.; Oberlack, H.; Schacht, P.; Scholz, S.; Wiedenmann, W.; Davier, M.; Grivaz, J.F.; Haissinski, J.; Journe, V.; Le Diberder, F.; Veillet, J.J.; Cozzika, G.; Ducros, Y.; Alexander, G.; Beck, A.; Bella, G.; Grunhaus, J.; Klatchko, A.; Levy, A.; Milstene, C.

1990-10-01

The intermittency structure of multihadronic e + e - annihilation is analyzed by evaluating the factorial moments F 2 -F 5 in 3-dimensional Lorentz invariant phase space as a function of the resolution scale. We interpret our data in the language of fractal objects. It turns out that the fractal dimension depends on the resolution scale in a way that can be attributed to geometrical resolution effects and dynamical effects, such as the π 0 Dalitz decay. The LUND 7.2 hadronization model provides an excellent description of the data. There is no indication of unexplained multiplicity fluctuations in small phase space regions. (orig.)

Epidemiology and Susceptibility of 3,051 Staphylococcus aureus Isolates from 25 University Hospitals Participating in the European SENTRY Study

OpenAIRE

Fluit, A. C.; Wielders, C. L. C.; Verhoef, J.; Schmitz, F.-J.

2001-01-01

A total of 3,051 methicillin-susceptible Staphylococcus aureus (MSSA) isolates and methicillin-resistant S. aureus (MRSA) isolates in Europe were compared. MRSA isolates constituted 25% of all isolates and were more prevalent in southern Europe. MRSA isolates appeared to be more prevalent in intensive care units than in outpatient departments. Only a small minority of MSSA isolates were multidrug resistant, whereas the majority of MRSA isolates were multidrug resistant.

Assessment of Optical Turbulence Profiles Derived From Probabilistic Climatology

Science.gov (United States)

2007-03-01

654.3.1 Transformed Data Results . . . . . . . . . . . . 664.3.2 Untransformed Data Results . . . . . . . . . . . 704.4 Application of ...the needed repower to destroy surface based enemy targets.Courtesy of Boeing Corporation. http://www.boeing.com/news/ fea-ture/aa2004/backgrounders...medium is cornerstone to successful employ-ment of these HELs. 1.3 Introduction to Optical Turbulence Lethal application of directed energy repower

e-Drug3D: 3D structure collections dedicated to drug repurposing and fragment-based drug design.

Science.gov (United States)

Pihan, Emilie; Colliandre, Lionel; Guichou, Jean-François; Douguet, Dominique

2012-06-01

In the drug discovery field, new uses for old drugs, selective optimization of side activities and fragment-based drug design (FBDD) have proved to be successful alternatives to high-throughput screening. e-Drug3D is a database of 3D chemical structures of drugs that provides several collections of ready-to-screen SD files of drugs and commercial drug fragments. They are natural inputs in studies dedicated to drug repurposing and FBDD. e-Drug3D collections are freely available at http://chemoinfo.ipmc.cnrs.fr/e-drug3d.html either for download or for direct in silico web-based screenings.

Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation of MC3T3-E1 osteoblastic cells

International Nuclear Information System (INIS)

Kimira, Yoshifumi; Ogura, Kana; Taniuchi, Yuri; Kataoka, Aya; Inoue, Naoki; Sugihara, Fumihito; Nakatani, Sachie; Shimizu, Jun; Wada, Masahiro; Mano, Hiroshi

2014-01-01

Highlights: • Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization. • Pro-Hyp significantly increased alkaline phosphatase activity. • Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Col1α1. - Abstract: Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Col1α1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Collα1 gene expression

Unmanned Bomber: The Effects It Will Bring to the Nuclear Triad

Science.gov (United States)

2014-06-01

New York, NY: Avery Trade, 2011), 41. 16 Boeing, History of B-2 Spirit , http://www.boeing.com/boeing/history/boeing/ b2 .page retrieved on January...factsheets/start1 retrieved on February 23, 2014 Boeing, History of B-2 Spirit , http://www.boeing.com/boeing/history/boeing/ b2 .page retrieved on

Tetraaquatetrakis{μ3-3,3′-[(E,E-ethane-1,2-diylbis(nitrilomethylidyne]benzene-1,2-diolato}octazinc(II N,N-dimethylformamide hexasolvate

Directory of Open Access Journals (Sweden)

Lan-Sun Zheng

2009-12-01

Full Text Available The asymmetric unit of the title compound [Zn8(C16H12N2O44(H2O4]·6C3H7NO, consists of eight ZnII cations, four tetravalent anionic ligands, L4− (L4− = 3,3′-(1E,1′E-(ethane-1,2-diylbis(azan-1-yl-1-ylidenebis(methan-1-yl-1-ylidenedibenzene-1,2-bis(olate, four coordinated water molecules and six N,N-dimethylformamide solvate molecules. The coordination complex comprises an octanuclear ZnII unit with its ZnII centers coordinated in two discrete distorted square-pyramidal geometries. Four ZnII atoms each coordinate to two nitrogen atoms and two phenolate oxygen atoms from an individual L4− ligand and one coordinated water molecule. The other four ZnII atoms each bind to five phenolate oxygen atoms from three different L4− ligands. In the crystal structure, the ZnII complex unit, coordinated water molecules and dimethylformamide solvate molecules are linked via O—H...O and C—H...O hydrogen bonds. Molecules are connected by additional intermolecular O—H...O and C—H...O hydrogen bonds, forming an extensive three dimensional framework.

E3 ubiquitin ligases as drug targets and prognostic biomarkers in melanoma

Directory of Open Access Journals (Sweden)

Kristina Bielskienė

2015-01-01

E3 ligases are of interest as drug targets for their ability to regulate proteins stability and functions. Compared to the general proteasome inhibitor bortezomib, which blocks the entire protein degradation, drugs that target a particular E3 ligase are expected to have better selectivity with less associated toxicity. Components of different E3 ligases complexes (FBW7, MDM2, RBX1/ROC1, RBX2/ROC2, cullins and many others are known as oncogenes or tumor suppressors in melanomagenesis. These proteins participate in regulation of different cellular pathways and such important proteins in cancer development as p53 and Notch. In this review we summarized published data on the role of known E3 ligases in the development of melanoma and discuss the inhibitors of E3 ligases as a novel approach for the treatment of malignant melanomas.

A recombinant E1-deleted porcine adenovirus-3 as an expression vector

International Nuclear Information System (INIS)

Zakhartchouk, Alexander; Zhou Yan; Tikoo, Suresh Kumar

2003-01-01

Replication-defective E1-deleted porcine adenoviruses (PAVs) are attractive vectors for vaccination. As a prerequisite for generating PAV-3 vectors containing complete deletion of E1, we transfected VIDO R1 cells (fetal porcine retina cells transformed with E1 region of human adenovirus 5) with a construct containing PAV-3 E1B large coding sequences under the control of HCMV promoter. A cell line named VR1BL could be isolated that expressed E1B large of PAV-3 and also complemented PAV214 (E1A+E1B small deleted). The VR1BL cells could be efficiently transfected with DNA and allowed the rescue and propagation of recombinant PAV507 containing a triple stop codon inserted in the E1B large coding sequence. In addition, recombinant PAV227 containing complete deletion of E1 (E1A+E1B small + E1B large ) could be successfully rescued using VR1BL cell line. Recombinant PAV227 replicated as efficiently as wild-type in VR1BL cells but not in VIDO R1 cells, suggesting that E1B large was essential for replication of PAV-3. Next, we constructed recombinant PAV219 by inserting green fluorescent (GFP) protein gene flanked by a promoter and a poly(A) in the E1 region of the PAV227 genome. We demonstrated that PAV219 was able to transduce and direct expression of GFP in some human cell lines

LEP3: A High Luminosity e+e- Collider to Study the Higgs Boson

CERN Document Server

Blondel, A U; Assmann, R W; Butterworth, A; Janot, P; Jimenez, J M; Grojean, C; Milanese, A; Modena, M; Osborne, J A; Zimmermann, F; Piekarz, H; Oide, K; Yokoya, K; Ellis, J; Klute, M; Zanetti, M; Velasco, M; Telnov, V; Rivkin, L; Cai, Y

2012-01-01

The LHC experiments have discovered a new particle with a mass around 125 GeV that is a strong candidate for the scalar Higgs boson expected in the Standard Model. An e+e− collider operating close to the ZH threshold (at a centre-of-mass energy of 240 GeV) could be the tool of choice for studying this unique particle in detail. We present here the concept of a storage ring collider, which we call LEP3. Preliminary studies show that at a centre-of-mass energy of 240 GeV, near-constant luminosities of 1034 cm-2s-1 are possible in up to four collision points, while respecting a number of constraints including beamstrahlung limits. With an integrated luminosity of 100fb-1 per year and per interaction point, 20,000 e+e- - ZH events would be produced per year and per experiment. LEP3 could also operate in multi-bunch mode at the Z resonance, with luminosities of several×1035cm-2s-1, yielding O(1011) Z decays per year, as well as just above the WW threshold, potentially improving our knowledge of W and Z propert...

A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism

Directory of Open Access Journals (Sweden)

Meera Shah

2016-08-01

Full Text Available The multi-subunit eukaryotic translation initiation factor eIF3 is thought to assist in the recruitment of ribosomes to mRNA. The expression of eIF3 subunits is frequently disrupted in human cancers, but the specific roles of individual subunits in mRNA translation and cancer remain elusive. Using global transcriptomic, proteomic, and metabolomic profiling, we found a striking failure of Schizosaccharomyces pombe cells lacking eIF3e and eIF3d to synthesize components of the mitochondrial electron transport chain, leading to a defect in respiration, endogenous oxidative stress, and premature aging. Energy balance was maintained, however, by a switch to glycolysis with increased glucose uptake, upregulation of glycolytic enzymes, and strict dependence on a fermentable carbon source. This metabolic regulatory function appears to be conserved in human cells where eIF3e binds metabolic mRNAs and promotes their translation. Thus, via its eIF3d-eIF3e module, eIF3 orchestrates an mRNA-specific translational mechanism controlling energy metabolism that may be disrupted in cancer.

Ghrelin inhibits the apoptosis of MC3T3-E1 cells through ERK and AKT signaling pathway

Energy Technology Data Exchange (ETDEWEB)

Liang, Qiu-Hua; Liu, Yuan; Wu, Shan-Shan; Cui, Rong-Rong; Yuan, Ling-Qing, E-mail: allenylq@hotmail.com; Liao, Er-Yuan, E-mail: eyliao@21cn.com

2013-11-01

Ghrelin is a 28-amino-acid peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR) and strongly stimulates the release of growth hormone from the hypothalamus–pituitary axis. Previous studies have identified the important physiological effects of ghrelin on bone metabolism, such as regulating proliferation and differentiation of osteoblasts, independent of GH/IGF-1 axis. However, research on effects and mechanisms of ghrelin on osteoblast apoptosis is still rare. In this study, we identified expression of GHSR in MC3T3-E1 cells and determined the effects of ghrelin on the apoptosis of osteoblastic MC3T3-E1 cells and the mechanism involved. Our data demonstrated that ghrelin inhibited the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, as determined by terminal deoxynucleotidyl transferase-mediated deoxyribonucleotide triphosphate nick end-labeling (TUNEL) and ELISA assays. Moreover, ghrelin upregulated Bcl-2 expression and downregulated Bax expression in a dose-dependent manner. Our study also showed decreased activated caspase-3 activity under the treatment of ghrelin. Further study suggested that ghrelin stimulated the phosphorylation of ERK and AKT. Pretreatment of cells with the ERK inhibitor PD98059, PI3K inhibitor LY294002, and GHSR-siRNA blocked the ghrelin-induced activation of ERK and AKT, respectively; however, ghrelin did not stimulate the phosphorylation of p38 or JNK. PD90859, LY294002 and GHSR-siRNA attenuated the anti-apoptosis effect of ghrelin in MC3T3-E1 cells. In conclusion, ghrelin inhibits the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, which may be mediated by activating the GHSR/ERK and GHSR/PI3K/AKT signaling pathways. - Highlights: • We explored the effects of ghrelin on serum deprivation-induced MC3T3-E1 cells apoptosis. • Both ELISA and TUNEL were used to detect the apoptosis. • The receptor of ghrelin, GHSR, was expressed in MC3T3-E1

Ghrelin inhibits the apoptosis of MC3T3-E1 cells through ERK and AKT signaling pathway

International Nuclear Information System (INIS)

Liang, Qiu-Hua; Liu, Yuan; Wu, Shan-Shan; Cui, Rong-Rong; Yuan, Ling-Qing; Liao, Er-Yuan

2013-01-01

Ghrelin is a 28-amino-acid peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR) and strongly stimulates the release of growth hormone from the hypothalamus–pituitary axis. Previous studies have identified the important physiological effects of ghrelin on bone metabolism, such as regulating proliferation and differentiation of osteoblasts, independent of GH/IGF-1 axis. However, research on effects and mechanisms of ghrelin on osteoblast apoptosis is still rare. In this study, we identified expression of GHSR in MC3T3-E1 cells and determined the effects of ghrelin on the apoptosis of osteoblastic MC3T3-E1 cells and the mechanism involved. Our data demonstrated that ghrelin inhibited the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, as determined by terminal deoxynucleotidyl transferase-mediated deoxyribonucleotide triphosphate nick end-labeling (TUNEL) and ELISA assays. Moreover, ghrelin upregulated Bcl-2 expression and downregulated Bax expression in a dose-dependent manner. Our study also showed decreased activated caspase-3 activity under the treatment of ghrelin. Further study suggested that ghrelin stimulated the phosphorylation of ERK and AKT. Pretreatment of cells with the ERK inhibitor PD98059, PI3K inhibitor LY294002, and GHSR-siRNA blocked the ghrelin-induced activation of ERK and AKT, respectively; however, ghrelin did not stimulate the phosphorylation of p38 or JNK. PD90859, LY294002 and GHSR-siRNA attenuated the anti-apoptosis effect of ghrelin in MC3T3-E1 cells. In conclusion, ghrelin inhibits the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, which may be mediated by activating the GHSR/ERK and GHSR/PI3K/AKT signaling pathways. - Highlights: • We explored the effects of ghrelin on serum deprivation-induced MC3T3-E1 cells apoptosis. • Both ELISA and TUNEL were used to detect the apoptosis. • The receptor of ghrelin, GHSR, was expressed in MC3T3-E1

Reaction (γ,2e) and (e,3e) as probe of electron correlation in atoms

International Nuclear Information System (INIS)

Amusia, M.Y.

1995-01-01

Cross sections of the (γ,2e) and (e,3e) reactions contain information about the two vacancy-energy spectrum and electron-pair correlations in initial and final states of the target atom. Physical pictures of these processes are presented for two- and many-electron atoms. The simplest mechanisms are discussed, demonstrating some features which await experimental confirmation. Attention is given to high photon energy and the relativistic energy region of these reactions. The energy distribution of outgoing relativistic electrons is qualitatively different from the nonrelativistic case. The origin and types of corrections to the simplest mechanisms, and possible means of their detection, are discussed. In addition, the role of different resonances: shape, giant, autoionizational, and Feshbach-type are considered. Results of calculations are compared with experimental data, mainly on double photoionization cross sections. Different possible objects as targets for the reactions are considered, including negative ions, excited atoms, molecules, and clusters. The modification of these reactions due to photon emission is discussed. The future of the domain is outlined

Reactions (γ,2e) and (e,3e) as probes of electronic correlations in atoms

International Nuclear Information System (INIS)

Amusia, M.Ya.

1993-01-01

Cross sections of the (γ,2e) and (e,3e) reactions carry information on two vacancy energy spectrum and on electron pair correlations in initial and final states of the target atom. Physical pictures of these processes are presented for two- and many-electron atoms. Simplest mechanisms of them are discussed, demonstrating some features which are waiting for experimental confirmation. Attention is given to high photon energy and even to relativistic energy region of these reactions. The energy distribution of outgoing relativistic electrons is qualitatively different from what it is for the nonrelativistic case. Origin and types of corrections to the simplest mechanisms and possible means of their detection are discussed. Role of different resonances: shape, giant, autoionizational, and Feschbach-type are considered. Results of calculations are compared with experimental data, mainly on double photoionization cross sections. Different possible objects as targets for the reactions are mentioned, including negative ions, excited atoms, molecules and clusters. Modification of the type of these reactions due to rather probable emission of the photon is discussed. Future of the domain is outlined. (orig.)

Crystal structure of (2E,3E-N2,N3-bis(3-ethyl-[1,1′-biphenyl]-4-ylbutane-2,3-diimine

Directory of Open Access Journals (Sweden)

Yan Zhao

2015-04-01

Full Text Available In the title compound, C32H32N2, synthesized by the condensation reaction of 2-ethyl-4-phenylaniline and 2,3-butanedione, the conformation about the C=N bonds is E and the substituted biphenyl units are trans to one another. In the two biphenyl ring systems, the planes of the two rings are inclined to one another by 25.25 (19 and 28.01 (19°. The planes of the ethyl-substituted benzene rings are inclined to one another by 20.23 (19° and to the mean plane of the butane-2,3-diimine unit [maximum deviation = 0.014 (4 Å] by 83.19 (19 and 63.38 (19°. In the crystal, molecules are linked by C—H...π interactions, forming sheets lying parallel to (101.

A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism.

Science.gov (United States)

Shah, Meera; Su, Dan; Scheliga, Judith S; Pluskal, Tomáš; Boronat, Susanna; Motamedchaboki, Khatereh; Campos, Alexandre Rosa; Qi, Feng; Hidalgo, Elena; Yanagida, Mitsuhiro; Wolf, Dieter A

2016-08-16

The multi-subunit eukaryotic translation initiation factor eIF3 is thought to assist in the recruitment of ribosomes to mRNA. The expression of eIF3 subunits is frequently disrupted in human cancers, but the specific roles of individual subunits in mRNA translation and cancer remain elusive. Using global transcriptomic, proteomic, and metabolomic profiling, we found a striking failure of Schizosaccharomyces pombe cells lacking eIF3e and eIF3d to synthesize components of the mitochondrial electron transport chain, leading to a defect in respiration, endogenous oxidative stress, and premature aging. Energy balance was maintained, however, by a switch to glycolysis with increased glucose uptake, upregulation of glycolytic enzymes, and strict dependence on a fermentable carbon source. This metabolic regulatory function appears to be conserved in human cells where eIF3e binds metabolic mRNAs and promotes their translation. Thus, via its eIF3d-eIF3e module, eIF3 orchestrates an mRNA-specific translational mechanism controlling energy metabolism that may be disrupted in cancer. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

A three-dimensional model of the atmospheric chemistry of E and Z-CF3CH=CHCl (HCFO-1233(zd) (E/Z))

Science.gov (United States)

Sulbaek Andersen, Mads P.; Schmidt, Johan A.; Volkova, Aleksandra; Wuebbles, Donald J.

2018-04-01

Using a 3-dimensional global atmospheric chemistry and transport model we investigated the atmospheric degradation of HCFO-1233zd(E), E-CF3CH=CHCl, a commercially important, new hydrofluorocarbon replacement compound. Atmospheric degradation of E-CF3CH=CHCl is initiated by reaction with OH radicals, which leads to several chemical oxidation products. Dissemination of these oxidation products to the environment is of concern due to the possible formation of trifluoroacetic acid (TFA) as a degradation product. The model indicates that the average global yield of TFA from atmospheric processing of E-CF3CH=CHCl is approximately 2%. The annually averaged atmospheric lifetime of E-CF3CH=CHCl was found to be approximately 36 days (12 days for Z-CF3CH=CHCl). As E-CF3CH=CHCl is short lived, by far the majority of its Cl atoms will be released and deposited in the lower atmosphere, and the impact on stratospheric ozone is insignificant. An Ozone Depletion Potential of 0.00030 was determined. The Photochemical Ozone Creation Potential was evaluated and a value of 3.6 determined. Finally, we derive a Global Warming Potential for E-CF3CH=CHCl for a 100 year time horizon of model.

The Midlatitude Continental Convective Clouds Experiment (MC3E)

Energy Technology Data Exchange (ETDEWEB)

Jensen, Mark P.; Petersen, Walt A.; Bansemer, Aaron; Bharadwaj, Nitin; Carey, Larry; Cecil, D. J.; Collis, Scott M.; Del Genio, Anthony D.; Dolan, Brenda A.; Gerlach, J.; Giangrande, Scott; Heymsfield, Andrew J.; Heymsfield, Gerald; Kollias, Pavlos; Lang, T. J.; Nesbitt, Steve W.; Neumann, Andrea; Poellot, M. R.; Rutledge, Steven A.; Schwaller, Mathew R.; Tokay, Ali; Williams, C. R.; Wolff, D. B.; Xie, Shaocheng; Zipser, Edward J.

2016-10-18

The Midlatitude Continental Convective Clouds Experiment (MC3E), a field program jointly led by the U.S. Department of Energy’s Atmospheric Radiation Measurement program and the NASA Global Precipitation Measurement (GPM) Mission, was conducted in south-central Oklahoma during April – May 2011. MC3E science objectives were motivated by the need to improve understanding of midlatitude continental convective cloud system lifecycles, microphysics, and GPM precipitation retrieval algorithms. To achieve these objectives a multi-scale surface- and aircraft-based in situ and remote sensing observing strategy was employed. A variety of cloud and precipitation events were sampled during the MC3E, of which results from three deep convective events are highlighted. Vertical structure, air motions, precipitation drop-size distributions and ice properties were retrieved from multi-wavelength radar, profiler, and aircraft observations for an MCS on 11 May. Aircraft observations for another MCS observed on 20 May were used to test agreement between observed radar reflectivities and those calculated with forward-modeled reflectivity and microwave brightness temperatures using in situ particle size distributions and ice water content. Multi-platform observations of a supercell that occurred on 23 May allowed for an integrated analysis of kinematic and microphysical interactions. A core updraft of 25 ms-1 supported growth of hail and large rain drops. Data collected during the MC3E campaign is being used in a number of current and ongoing research projects and is available through the DOE ARM and NASA data archives.

Electromagnetic α3 contributions to e+e- - annihilation into fermions in the electroweak theory

International Nuclear Information System (INIS)

Bardin, D.Yu.; Riemann, T.

1987-01-01

Analytic expressions are obtained for the completely integrated QEDα 3 contributions to the total cross section σ T and the integrated forward-backward asymmetry A FB in the process e + e - →f-barf(γ). The photons are assumed not to be observable. The calculation is performed in ultrarelativistic approximation over fermion masses, and the mass M Z and width Γ Z of the neutral intermediate vector boson are not treated as small parameters

Light Distribution in the E3 and E4 Scintillation Counters of the ATLAS Tile Calorimeter

CERN Document Server

Hsu, Catherine

2013-01-01

The Tile Calorimeter (TileCal) of the ATLAS experiment is an important component of the ATLAS calorimetry because they play a crucial role in the search for new particles. The E3 and E4 are crack scintillators of TileCal that extend into the gap region between the EM barrel and EM endcaps. They thus sample the energy of the EM showers produced by particles interacting with the dead material in the EM calorimeters and with the inner detector cables. This project focuses on the study of the light collection uniformity in the E3 and E4 scintillating tiles using low energy electrons as the ionising particles. It is important to have uniform light response in the tiles because it would ensure a good energy resolution for the dead region. However, many factors affect the uniform light collection within the scintillating tiles.

Dal “modello 3D” alla “scena 3D”. Prospettive e opportunità per la valorizzazione del patrimonio culturale architettonico e urbano.

Directory of Open Access Journals (Sweden)

Elena Ippoliti

2010-12-01

Full Text Available La multimedialità è fatta di “ricchezza audiovisiva, profondità conoscitiva e informativa, interattività”[i] e, ancora, un prodotto multimediale dovrebbe essere “ricco, profondo e mobile, avvincente come un film, sistematico come un libro e interattivo come un videogioco”[ii]. A partire da tali definizioni, il contributo proposto intende indagare su alcune delle opportunità che le più recenti innovazioni tecnologiche offrono per l’ampliamento e la diffusione delle conoscenze sul patrimonio culturale, in particolare soffermandosi sulle applicazioni che privilegiano la “scena digitale 3D” del bene culturale, architettonico e urbano, sia in quanto interfaccia di accesso ai contenuti culturali e sia in quanto luogo in cui si costruiscono informazioni. Tale modalità di approccio, analoga e interattiva, incardinata sul coinvolgimento sensoriale ed emozionale, si costituisce di fatto come un valore aggiunto per la comunicazione, la fruizione e l’elaborazione di informazioni e contenuti, qualificandosi utilmente per la valorizzazione del patrimonio culturale. [i] Negroponte N., Essere digitali, Sperling & Kupfer, Milano, 2004. [ii] Maragliano R., Manuale di didattica multimediale, Laterza, Roma-Bari, 1994. p.43.

Editorial L&E, v.8, n.3, 2014

Directory of Open Access Journals (Sweden)

Rede Conpadre

2014-09-01

Full Text Available Editorial L&E, v.8, n.3, 2014. É sempre uma satisfação apresentar mais um número da Revista Labor & Engenho [ISSN:2176-8846]. Neste, o leitor poderá apreciar 7 excelentes artigos que juntos refletem diferentes aspectos das benéficas conexões que se estabelecem entre Patrimônio e Desenvolvimento Regional. Dos 7 artigos que compõem o número 3 do volume 8 da Revista L&E, 3 são de autores brasileiros (sendo 2 no idioma português, 1 em inglês; 2 são de autores argentinos (em espanhol; 1 de autores cubanos (também em espanhol; e 1 de autor francês (publicado no idioma original. Neste número temos, pois, a satisfação de publicar, pela primeira vez na Revista L&E, um artigo proveniente de Cuba e outro da França. Damo-lhes as boas vindas e esperamos por novos artigos originários de países do Caribe ou da América Central, assim como esperamos atrair novos pesquisadores e leitores francófonos, já que o idioma francês era o único dentre os 5 idiomas da Revista L&E que ainda não havia sido publicado neste Periódico. Este número se inicia com o artigo de Gercinair Silvério Gandara, que convida a uma deliciosa visita às águas doces do lago de Sobradinho, no rio São Francisco, onde dormita o patrimônio construído das cidades-beira de Remanso e Pilão Arcado. O trabalho faz parte de sua mais recente pesquisa de Pós-Doutorado, financiada pela CAPES. A autora salienta que a força geradora sociocultural das cidades-beira e sua sinergia com o espaço geográfico ressaltam a importância do Patrimônio Ambiental. Em seguida apresenta-se um artigo sobre as frentes pioneiras do Brasil, enfocando a Amazônia. Não poderia ser mais simbólico e significativo o artigo de Hervé Théry, o primeiro publicado em francês pela Revista Labor & Engenho. Nele o autor, titular da Cátedra Pierre Monbeig (que leva o nome do geógrafo francês que participou da fundação da USP, revisita um de seus trabalhos que seguiram a orientação do pr

Aspects of radiative K{sup +}{sub e3} decays

Energy Technology Data Exchange (ETDEWEB)

Kubis, B. [Universitaet Bonn, Helmholtz-Institut fuer Strahlen- und Kernphysik, Bonn (Germany); Mueller, E.H. [Universitaet Bonn, Helmholtz-Institut fuer Strahlen- und Kernphysik, Bonn (Germany); University of Edinburgh, School of Physics, Edinburgh (United Kingdom); Gasser, J.; Schmid, M. [Universitaet Bern, Institut fuer theoretische Physik, Bern (Switzerland)

2007-04-15

We re-investigate the radiative charged kaon decay K{sup {+-}}{yields}{pi}{sup 0}e{sup {+-}}{nu}{sub e}{gamma} [K{sub e3{gamma}}{sup {+-}}] in chiral perturbation theory, merging the chiral expansion with Low's theorem. We thoroughly analyze the precision of the predicted branching ratio relative to the non-radiative decay channel. Structure dependent terms and their impact on differential decay distributions are investigated in detail, and the possibility to see effects of the chiral anomaly in this decay channel is emphasized. (orig.)

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

Science.gov (United States)

Watanabe, Satoshi; Hideshima, Rumiko; Xia, Zhengjun; Tsubokura, Yasutaka; Sato, Shusei; Nakamoto, Yumi; Yamanaka, Naoki; Takahashi, Ryoji; Ishimoto, Masao; Anai, Toyoaki; Tabata, Satoshi; Harada, Kyuya

2009-01-01

Photosensitivity plays an essential role in the response of plants to their changing environments throughout their life cycle. In soybean [Glycine max (L.) Merrill], several associations between photosensitivity and maturity loci are known, but only limited information at the molecular level is available. The FT3 locus is one of the quantitative trait loci (QTL) for flowering time that corresponds to the maturity locus E3. To identify the gene responsible for this QTL, a map-based cloning strategy was undertaken. One phytochrome A gene (GmPhyA3) was considered a strong candidate for the FT3 locus. Allelism tests and gene sequence comparisons showed that alleles of Misuzudaizu (FT3/FT3; JP28856) and Harosoy (E3/E3; PI548573) were identical. The GmPhyA3 alleles of Moshidou Gong 503 (ft3/ft3; JP27603) and L62-667 (e3/e3; PI547716) showed weak or complete loss of function, respectively. High red/far-red (R/FR) long-day conditions enhanced the effects of the E3/FT3 alleles in various genetic backgrounds. Moreover, a mutant line harboring the nonfunctional GmPhyA3 flowered earlier than the original Bay (E3/E3; PI553043) under similar conditions. These results suggest that the variation in phytochrome A may contribute to the complex systems of soybean flowering response and geographic adaptation. PMID:19474204

(e, 3e) Test on e–e correlations in helium

Indian Academy of Sciences (India)

£Present address: S.I.E.T., Dulehra Marg, Modipuram, Meerut 250 110, India ... A proper accounting of e–e correlation in the final state which contains three electrons in the field .... The results are however not very sensitive to its value. αb. , αc.

Finding the 3-gluon vertex from 4-jet events in e+e- annihilation

International Nuclear Information System (INIS)

Bengtsson, M.

1988-05-01

Although the 3-gluon coupling is a necessary ingredient for QCD to be asymptotically free, conclusive experimental evidence is still lacking. We make here a comprehensive and systematic study of the possibilities of finding it in e + e - annihilation at the Z 0 resonance. Emphasis is put on observables of 4-jet events which are sensitive to the specific helicity structure of the processes g → gg and g → qanti q. These observables give a quantitative and qualitative difference between QCD and abelian models, making it straightforward to confirm the nonabelian nature of the theory of strong interactions. (orig.)

Identification of Arabidopsis MYB56 as a novel substrate for CRL3(BPM) E3 ligases.

Science.gov (United States)

Chen, Liyuan; Bernhardt, Anne; Lee, JooHyun; Hellmann, Hanjo

2015-02-01

Controlled stability of proteins is a highly efficient mechanism to direct diverse processes in living cells. A key regulatory system for protein stability is given by the ubiquitin proteasome pathway, which uses E3 ligases to mark specific proteins for degradation. In this work, MYB56 is identified as a novel target of a CULLIN3 (CUL3)-based E3 ligase. Its stability depends on the presence of MATH-BTB/POZ (BPM) proteins, which function as substrate adaptors to the E3 ligase. Genetic studies have indicated that MYB56 is a negative regulator of flowering, while BPMs positively affect this developmental program. The interaction between BPMs and MYB56 occurs at the promoter of FLOWERING LOCUS T (FT), a key regulator in initiating flowering in Arabidopsis, and results in instability of MYB56. Overall the work establishes MYB transcription factors as substrates of BPM proteins, and provides novel information on components that participate in controlling flowering time in plants. Copyright © 2015 The Author. Published by Elsevier Inc. All rights reserved.

Attosecond sublevel beating and nonlinear dressing on the 3d-to-5p and 3p-to-5s core-transitions at 91.3 eV and 210.4 eV in krypton.

Science.gov (United States)

Seres, Enikoe; Seres, Jozsef; Namba, Shinichi; Afa, John; Serrat, Carles

2017-12-11

Applying extreme ultraviolet (XUV) transient absorption spectroscopy, the dynamics of the two laser dressed transitions 3d 5/2 -to-5p 3/2 and 3p 3/2 -to-5s 1/2 at photon energies of 91.3 eV and 210.4 eV were examined with attosecond temporal resolution. The dressing process was modeled with density matrix equations which are found to describe very accurately both the experimentally observed transmission dynamics and the linear and nonlinear dressing oscillations at 0.75 PHz and 1.5 PHz frequencies. Furthermore, using Fourier transform XUV spectroscopy, quantum beats from the 3d 5/2 -3d 3/2 and 3p 3/2 -3p 1/2 sublevels at 0.3 PHz and 2.0 PHz were experimentally identified and resolved.

GPM GROUND VALIDATION OKLAHOMA CLIMATOLOGICAL SURVEY MESONET MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validation Oklahoma Climatological Survey Mesonet MC3E data were collected during the Midlatitude Continental Convective Clouds Experiment (MC3E) in...

Protective effect of Edaravone against hypoxia-induced cytotoxicity in osteoblasts MC3T3-E1 cells.

Science.gov (United States)

Cao, Bo; Chai, Chunxiang; Zhao, Sishun

2015-12-01

Edaravone is a newly developed clinical medicine for the treatment of acute cerebral infarction. Reduced blood supply to bones (hypoxia) has been involved in the pathological development of osteoporosis. In this study, we investigated the effect of Edaravone and its latent mechanism on hypoxia-induced cell toxicity in MC3T3-E1 cells. Cell viability was determined by the 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Intracellular reactive oxygen species (ROS) and nitric oxide (NO) were determined by the fluorescence dyes 2',7'-dichlorofluorescein diacetate (DCFH-DA) and 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM DA), respectively. mRNA and proteins were determined by real-time polymerase chain reaction and Western blot analysis, respectively. Edaravone significantly restored the hypoxia-induced reduction of MC3T3-E1 cell viability and inhibited lactate dehydrogenase release. In addition, we found that Edaravone inhibits the generation of ROS and NO. Hoechst staining results indicated that the nuclear condensation characteristic of apoptosis was increased in MC3T3-E1 cells after hypoxia exposure, which was significantly suppressed by Edaravone treatment. Mechanistically, we found that Edaravone markedly reduced the expression of cleaved caspase-3 and blunted the release of cytochrome c. These findings strongly suggested that Edaravone suppresses hypoxia-induced cytotoxicity in MC3T3-E1 cells. The pleiotropic effects of Edaravone on hypoxia exposure in osteoblasts suggest potential antiosteoporosis mechanisms of Edaravone. © 2015 International Union of Biochemistry and Molecular Biology.

Plasma lipoproteins in familial dysbetalipoproteinemia associated with apolipoproteins E2 (Arg158 -->Cys), E3-Leiden, and E2 (Lys146-->Gln), and effects of treatment with simvastatin

NARCIS (Netherlands)

Zhao, S.P.; Smelt, A.H.; Maagdenberg, A.M. van den; Tol, A. van; Vroom T.F.; Gevers Leuven, J.A.; Frants, R.R.; Havekes, L.M.; Laarse, A. van der; Hooft, F.M. van 't

1994-01-01

Using a density-gradient ultracentrifugation technique, we analyzed in detail the plasma lipoprotein profiles of 18 patients with familial dysbetalipoproteinemia (FD) who had apolipoprotein (apo) E2(Arg158-->Cys) homozygosity (the E2-158 variant, n = 6), apoE3-Leiden heterozygosity (the E3-Leiden

Aircraft Fire Sentry. Volume 2. Appendices

Science.gov (United States)

1993-01-01

including New Mexico Engineering Research Institute (NMERI), National Institute of Standards and Technology (NIST), Wright-Patterson AFB, and AFESC at...Installation Remove the mounting bracket from the smoke alarm by depresing the release tab marked "PRESS" (see Figure 1. pg. 5). Pivot the bracket awey from and

BOPACE 3-D addendum: The Boeing plastic analysis capabilities for 3-dimensional solids using isoparametric finite elements

Science.gov (United States)

Vos, R. G.; Straayer, J. W.

1975-01-01

Modifications and additions incorporated into the BOPACE 3-D program are described. Updates to the program input data formats, error messages, file usage, size limitations, and overlay schematic are included.

Bioinformatics analysis identifies several intrinsically disordered human E3 ubiquitin-protein ligases

Directory of Open Access Journals (Sweden)

Wouter Boomsma

2016-02-01

Full Text Available The ubiquitin-proteasome system targets misfolded proteins for degradation. Since the accumulation of such proteins is potentially harmful for the cell, their prompt removal is important. E3 ubiquitin-protein ligases mediate substrate ubiquitination by bringing together the substrate with an E2 ubiquitin-conjugating enzyme, which transfers ubiquitin to the substrate. For misfolded proteins, substrate recognition is generally delegated to molecular chaperones that subsequently interact with specific E3 ligases. An important exception is San1, a yeast E3 ligase. San1 harbors extensive regions of intrinsic disorder, which provide both conformational flexibility and sites for direct recognition of misfolded targets of vastly different conformations. So far, no mammalian ortholog of San1 is known, nor is it clear whether other E3 ligases utilize disordered regions for substrate recognition. Here, we conduct a bioinformatics analysis to examine >600 human and S. cerevisiae E3 ligases to identify enzymes that are similar to San1 in terms of function and/or mechanism of substrate recognition. An initial sequence-based database search was found to detect candidates primarily based on the homology of their ordered regions, and did not capture the unique disorder patterns that encode the functional mechanism of San1. However, by searching specifically for key features of the San1 sequence, such as long regions of intrinsic disorder embedded with short stretches predicted to be suitable for substrate interaction, we identified several E3 ligases with these characteristics. Our initial analysis revealed that another remarkable trait of San1 is shared with several candidate E3 ligases: long stretches of complete lysine suppression, which in San1 limits auto-ubiquitination. We encode these characteristic features into a San1 similarity-score, and present a set of proteins that are plausible candidates as San1 counterparts in humans. In conclusion, our work

Cigarettes with different nicotine levels affect sensory perception and levels of biomarkers of exposure in adult smokers.

Science.gov (United States)

McKinney, Diana L; Frost-Pineda, Kimberly; Oldham, Michael J; Fisher, Michael T; Wang, Jingzhu; Gogova, Maria; Kobal, Gerd

2014-07-01

Few clinical studies involving cigarettes have provided a comprehensive picture of smoke exposure, test article characterization, and insights into sensory properties combined. The purpose of these pilot studies was to determine whether cigarettes with different levels of nicotine but similar tar levels would affect sensory experience or smoking behavior so as to significantly alter levels of selected biomarkers of exposure (BOE). In 2 confined, double-blind studies, 120 adult smokers switched from Marlboro Gold cigarettes at baseline to either 1 of 2 lower nicotine cigarettes or 1 of 2 higher nicotine cigarettes and then to the other cigarette after 5 days. Urinary excretion of exposure biomarkers (nicotine equivalents [NE], total and free 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol [NNAL], 1-hydroxypyrene, and 3-hydroxypropyl mercapturic acid) as well as carboxyhemoglobin and plasma cotinine were measured at baseline, Day 5, and Day 10. Daily cigarette consumption was monitored and sensory characteristics were rated for each cigarette. With higher nicotine yield, urine NE, urine total NNAL, and plasma cotinine increased while nonnicotine BOE decreased without changes in cigarette consumption. In contrast, with lower nicotine yield, urine NE, urine total NNAL, and plasma cotinine dropped while nonnicotine BOE and cigarettes per day increased. Higher nicotine cigarettes were rated harsher and stronger than at baseline while lower nicotine cigarettes were less strong. All 4 test cigarettes were highly disliked. These studies demonstrate that abrupt increases or decreases in nicotine and the resulting sensory changes impact BOE through changes in intensity or frequency of smoking. © The Author 2014. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Free and nanoencapsulated vitamin D3 : effects on E-NTPDase and E-ADA activities in an animal model with induced arthritis.

Science.gov (United States)

da Silveira, Karine Lanes; da Silveira, Leonardo Lanes; Thorstenberg, Maria Luiza Prates; Cabral, Fernanda Licker; Castilhos, Livia Gelain; Rezer, João Felipe Peres; de Andrade, Diego Fontana; Beck, Ruy Carlos Ruver; Einloft Palma, Heloísa; de Andrade, Cinthia Melazzo; Pereira, Renata da Silva; Martins, Nara Maria Beck; Bertonchel Dos Santos, Claudia de Mello; Leal, Daniela Bitencourt Rosa

2016-06-01

The effect of vitamin D3 in oral solution (VD3 ) and vitamin D3 -loaded nanocapsules (NC-VD3 ) was analysed in animals with complete Freund's adjuvant (CFA) induced arthritis (AR). For this purpose, we evaluated scores for arthritis, thermal hyperalgesia and paw oedema, as well as histological analyses and measurements of the activity of the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) and ecto-adenosine deaminase (E-ADA) enzymes in rat lymphocytes. Haematological and biochemical parameters were also determined. The doses administered were 120 UI/day of VD3 and 15.84 UI/day of NC-VD3 . Fifteen days after the induction of AR, the groups were treated for 15 days with vitamin D3 . The results demonstrated that VD3 was able to reduce arthritis scores, thermal hyperalgesia and paw oedema in rats with CFA-induced arthritis. However, treatment with NC-VD3 did not reduce arthritis scores. The histological analyses showed that both formulations were able to reduce the inflammatory changes induced by CFA. The activity of E-NTPDase in rat lymphocytes was higher in the AR compared with the control group, while the activity of E-ADA was lower. This effect was reversed after the 15-day treatment. Data from this study indicates that both forms of vitamin D3 seem to contribute to decreasing the inflammatory process induced by CFA, possibly altering the activities of ectoenzymes. Copyright © 2016 John Wiley & Sons, Ltd. The effects promoted by both formulations of vitamin D3 , either in oral solution or nanoencapsulated form, strongly suggests the softening of the inflammatory process induced by complete Freund's adjuvant (CFA), possibly altering the E-NTPDase and E-ADA activities. However, it is known that vitamin D has a beneficial effect on the modulation of the immune system components responsible for the inflammatory process. Moreover, the establishment of responses to treatment with vitamin D3 may provide an alternative for inhibiting the proinflammatory

Estimate of dietary intake of chloropropanols (3-MCPD and 1,3-DCP and health risk assessment Estimativa da ingestão de cloropropanóis (3-MCPD e 1,3-DCP e avaliação do risco à saúde

Directory of Open Access Journals (Sweden)

Adriana Pavesi Arisseto

2013-02-01

Full Text Available Chloropropanols, including 3-monochloropropane-1,2-diol (3-MCPD and 1,3-dichloropropan-2-ol (1,3-DCP, comprise a group of chemical contaminants with carcinogenic and genotoxic properties. They have been found in a variety of processed foods and food ingredients, such as hydrolyzed vegetable protein, soy sauce, cereal-based products, malt-derived ingredients, and smoked foods. This study aimed to assess the dietary exposure to 3-MCPD and 1,3-DCP in Brazil and verify whether the presence of these substances in foods could represent health risks. The intake was calculated by combining data on food consumption, provided by the Consumer Expenditure Survey 2008-2009, with the levels of contaminant occurrence determined by gas chromatography-mass spectrometry. The exposure to 3-MCPD ranged from 0.06 to 0.51 µg.kg bw-1.day-1 considering average and high consumers, while the intake of 1,3-DCP was estimated to be 0.0036 µg.kg bw-1.day-1 in the worst case scenario evaluated. Based on these results, it was verified that the Brazilians' exposure to chloropropanols does not present a significant health risk. However, the consumption of specific foods containing high levels of 3-MCPD could exceed the provisional maximum tolerable daily intake of 2 µg.kg bw-1 established for this compound and, therefore, represent a potential concern.Os cloropropanóis, entre eles o 3-monocloropropano-1,2-diol (3-MCPD e o 1,3-dicloropropan-2-ol (1,3-DCP, compreendem um grupo de contaminantes químicos com propriedades carcinogênicas e genotóxicas, encontrados em diversos alimentos processados e ingredientes alimentícios, como proteína vegetal hidrolisada, molho de soja, produtos à base de cereais, ingredientes derivados de malte e alimentos defumados. Este trabalho teve como objetivo avaliar a exposição ao 3-MCPD e 1,3-DCP pela dieta no Brasil e verificar se a presença destes compostos em alimentos pode representar um risco à saúde da população. A ingestão foi

Review of e+e- experiments with PLUTO from 3 to 31 GeV

International Nuclear Information System (INIS)

Criegee, L.; Knies, G.

1981-07-01

The contributions of the PLUTO experiment to e + e - physics at collision energies in the range of 3-31 GeV are reviewed. The review briefly sketches the storage rings DORIS and PETRA at DESY, and describes the most important features of the PLUTO detector, of data processing, and of the analysis methods. It covers the physics results in the fields of electroweak interactions, of the heavy lepton tau and the search for still heavier leptons, of hadron production with evidence for quark and gluon jets, as well as multiparton effects, of UPSILON decays and their relation to QCD, and concludes with first results in high-energy photon-photon interactions. (orig.)

Complement C3 deficiency attenuates chronic hypoxia-induced pulmonary hypertension in mice.

Directory of Open Access Journals (Sweden)

Eileen M Bauer

Full Text Available Evidence suggests a role of both innate and adaptive immunity in the development of pulmonary arterial hypertension. The complement system is a key sentry of the innate immune system and bridges innate and adaptive immunity. To date there are no studies addressing a role for the complement system in pulmonary arterial hypertension.Immunofluorescent staining revealed significant C3d deposition in lung sections from IPAH patients and C57Bl6/J wild-type mice exposed to three weeks of chronic hypoxia to induce pulmonary hypertension. Right ventricular systolic pressure and right ventricular hypertrophy were increased in hypoxic vs. normoxic wild-type mice, which were attenuated in C3-/- hypoxic mice. Likewise, pulmonary vascular remodeling was attenuated in the C3-/- mice compared to wild-type mice as determined by the number of muscularized peripheral arterioles and morphometric analysis of vessel wall thickness. The loss of C3 attenuated the increase in interleukin-6 and intracellular adhesion molecule-1 expression in response to chronic hypoxia, but not endothelin-1 levels. In wild-type mice, but not C3-/- mice, chronic hypoxia led to platelet activation as assessed by bleeding time, and flow cytometry of platelets to determine cell surface P-selectin expression. In addition, tissue factor expression and fibrin deposition were increased in the lungs of WT mice in response to chronic hypoxia. These pro-thrombotic effects of hypoxia were abrogated in C3-/- mice.Herein, we provide compelling genetic evidence that the complement system plays a pathophysiologic role in the development of PAH in mice, promoting pulmonary vascular remodeling and a pro-thrombotic phenotype. In addition we demonstrate C3d deposition in IPAH patients suggesting that complement activation plays a role in the development of PAH in humans.

Wind-US Users Guide Version 3.0

Science.gov (United States)

Yoder, Dennis A.

2016-01-01

Wind-US is a computational platform which may be used to numerically solve various sets of equations governing physical phenomena. Currently, the code supports the solution of the Euler and Navier-Stokes equations of fluid mechanics, along with supporting equation sets governing turbulent and chemically reacting flows. Wind-US is a product of the NPARC Alliance, a partnership between the NASA Glenn Research Center (GRC) and the Arnold Engineering Development Complex (AEDC) dedicated to the establishment of a national, applications-oriented flow simulation capability. The Boeing Company has also been closely associated with the Alliance since its inception, and represents the interests of the NPARC User's Association. The "Wind-US User's Guide" describes the operation and use of Wind-US, including: a basic tutorial; the physical and numerical models that are used; the boundary conditions; monitoring convergence; the files that are read and/or written; parallel execution; and a complete list of input keywords and test options. For current information about Wind-US and the NPARC Alliance, please see the Wind-US home page at http://www.grc.nasa.gov/WWW/winddocs/ and the NPARC Alliance home page at http://www.grc.nasa.gov/WWW/wind/. This manual describes the operation and use of Wind-US, a computational platform which may be used to numerically solve various sets of equations governing physical phenomena. Wind-US represents a merger of the capabilities of four CFD codes - NASTD (a structured grid flow solver developed at McDonnell Douglas, now part of Boeing), NPARC (the original NPARC Alliance structured grid flow solver), NXAIR (an AEDC structured grid code used primarily for store separation analysis), and ICAT (an unstructured grid flow solver developed at the Rockwell Science Center and Boeing).

Identification of Arabidopsis MYB56 as a novel substrate for CRL3BPM E3 ligases.

Science.gov (United States)

Chen, Liyuan; Bernhardt, Anne; Lee, JooHyun; Hellmann, Hanjo

2014-10-24

Controlled stability of proteins is a highly efficient mechanism to direct diverse processes in living cells. A key regulatory system for protein stability is given by the ubiquitin proteasome pathway, which uses E3 ligases to mark specific proteins for degradation. In this work MYB56 is identified as a novel target of a CULLIN3 (CUL3)-based E3 ligase. Its stability depends on the presence of MATH-BTB/POZ (BPM) proteins, which function as substrate adaptors to the E3 ligase. Genetic studies pointed out that MYB56 is a negative regulator of flowering, while BPMs positively affect this developmental program. The interaction between BPMs and MYB56 occurs at the promoter of FLOWERING LOCUS T (FT), a key regulator in initiating flowering in Arabidopsis, and results in instability of MYB56. Overall the work establishes MYB transcription factors as substrates of BPM proteins, and provides novel information on components that participate in controlling the flowering time point in plants. © The Author 2014. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.

Investigation of Natural Gas Fugitive Leak Detection Using an Unmanned Aerial Vehicle

Science.gov (United States)

Yang, S.; Talbot, R. W.; Frish, M. B.; Golston, L.; Aubut, N. F.; Zondlo, M. A.

2017-12-01

The U.S is now the world's largest natural gas producer, of which methane (CH4) is the main component. About 2% of the CH4 is lost through fugitive leaks. This research is under the DOE Methane Observation Networks with Innovative Technology to Obtain Reductions (MONITOR) program of ARPA-E. Our sentry measurement system is composed of four state-of-the-art technologies centered around the RMLDTM (Remote Methane Leak Detector). An open path RMLDTM measures column-integrated CH4 concentration that incorporates fluctuations in the vertical CH4 distribution. Based on Backscatter Tunable Diode Laser Absorption Spectroscopy and Small Unmanned Aerial Vehicles, the sentry system can autonomously, consistently and cost-effectively monitor and quantify CH4 leakage from sites associated with natural gas production. This system provides an advanced capability in detecting leaks at hard-to-access sites (e.g., wellheads) compared to traditional manual methods. Automated leak detecting and reporting algorithms combined with wireless data link implement real-time leak information reporting. Early data were gathered to set up and test the prototype system, and to optimize the leak localization and calculation strategies. The flight pattern is based on a raster scan which can generate interpolated CH4 concentration maps. The localization and quantification algorithms can be derived from the plume images combined with wind vectors. Currently, the accuracy of localization algorithm can reach 2 m and the calculation algorithm has a factor of 2 accuracy. This study places particular emphasis on flux quantification. The data collected at Colorado and Houston test fields were processed, and the correlation between flux and other parameters analyzed. Higher wind speeds and lower wind variation are preferred to optimize flux estimation. Eventually, this system will supply an enhanced detection capability to significantly reduce fugitive CH4 emissions in the natural gas industry.

32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

Science.gov (United States)

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form 4881-3-R...

Small Ribosomal Protein RPS0 Stimulates Translation Initiation by Mediating 40S-Binding of eIF3 via Its Direct Contact with the eIF3a/TIF32 Subunit

Czech Academy of Sciences Publication Activity Database

Kouba, Tomáš; Dányi, István; Gunišová, Stanislava; Munzarová, Vanda; Vlčková, Vladislava; Cuchalová, Lucie; Neueder, A.; Milkereit, P.; Valášek, Leoš Shivaya

2012-01-01

Roč. 7, č. 7 (2012), e40464 E-ISSN 1932-6203 R&D Projects: GA ČR(CZ) GAP305/11/0172 Institutional research plan: CEZ:AV0Z50200510 Keywords : eIF3a/TIF32 * mRNAs * protein Subject RIV: EE - Microbiology, Virology Impact factor: 3.730, year: 2012

Ice Particle Transport Analysis With Phase Change for the E(sup 3) Turbofan Engine Using LEWICE3D Version 3.2

Science.gov (United States)

Bidwell, Colin, S.

2012-01-01

Ice Particle trajectory calculations with phase change were made for the Energy Efficient Engine (E(sup 3)) using the LEWICE3D Version 3.2 software. The particle trajectory computations were performed using the new Glenn Ice Particle Phase Change Model which has been incorporated into the LEWICE3D Version 3.2 software. The E(sup 3) was developed by NASA and GE in the early 1980 s as a technology demonstrator and is representative of a modern high bypass turbofan engine. The E(sup 3) flow field was calculated using the NASA Glenn ADPAC turbomachinery flow solver. Computations were performed for the low pressure compressor of the E(sup 3) for a Mach 0.8 cruise condition at 11,887 m assuming a standard warm day for ice particle sizes of 5, 20, and 100 microns and a free stream particle concentration of 0.3 g/cu m. The impingement efficiency results showed that as particle size increased average impingement efficiencies and scoop factors increased for the various components. The particle analysis also showed that the amount of mass entering the inner core decreased with increased particle size because the larger particles were less able to negotiate the turn into the inner core due to particle inertia. The particle phase change analysis results showed that the larger particles warmed less as they were transported through the low pressure compressor. Only the smallest 5 micron particles were warmed enough to produce melting and the amount of melting was relatively small with a maximum average melting fraction of 0.836. The results also showed an appreciable amount of particle sublimation and evaporation for the 5 micron particles entering the engine core (22 percent).

Phosphorylation in vitro of eukaryotic initiation factors IF-E2 and IF-E3 by protein kinases

DEFF Research Database (Denmark)

Issinger, O G; Benne, R; Hershey, J W

1976-01-01

Purified protein synthesis initiation factors IF-E2 and IF-E3 from rabbit reticulocytes were phosphorylated in vitro with protein kinases isolated from the same source. The highest levels of phosphorylation resulted from incubation of the factors with a cyclic nucleotide-independent protein kinase...

L'evoluzione della stampa 3D e le sue applicazioni in campo museale

OpenAIRE

Pignatelli, Francesco

2014-01-01

ItLa stampa 3D è passata dall'uso solo industriale al grande pubblico degli appassionati e dei maker. Questa evoluzione interessa anche i musei, che possono adottare le tecniche di stampa 3D per rendere più fruibili le loro collezioni, creare una maggiore interattività con il pubblico potenziale e generare nuovo business attraverso il merchandising. Più in futuro i musei andranno verso un modello in cui l'originale e la copia ad alta precisione ottenuta con la stampa 3D, e persino il suo mode...

Amperometric estimation of La3+ and Ce3+ with cupferron using d.m.e

International Nuclear Information System (INIS)

Pitre, K.S.; Lavale, S.C.

1982-01-01

Cupferron has been employed as a reagent for the amperometric titrations of La 3+ and Ce 3+ in very dilute solution using d.m.e. Hydrochloric acid (pH=2.75) has been used as supporting electrolyte. These titrations revealed cupferron to metal ratio of 1:1. Titrations are not hampered by the presence of a fairly large amount of Li + , Na + , K + , Mg 3+ , Zn 3+ , Al 3+ , Cl - , Br - , I - , NO 3 - , NO 2 - , CH 2 COO - and ClO 4 - ions. Furthermore, micro and ultramicro quantities of La 3+ and Ce 2+ have been successfully determined with an error of less than +- 0.70%. (author)

Quantum phase transition in the U(4) vibron model and the E(3) symmetry

International Nuclear Information System (INIS)

Zhang Yu; Hou Zhanfeng; Chen Huan; Wei Haiqing; Liu Yuxin

2008-01-01

We study the details of the U(3)-O(4) quantum phase transition in the U(4) vibron model. Both asymptotic analysis in the classical limit and rigorous calculations for finite boson number systems indicate that a second-order phase transition is still there even for the systems with boson number N ranging from tens to hundreds. Two kinds of effective order parameters, including E1 transition ratios B(E1:2 1 →1 1 )/B(E1:1 1 →0 1 ) and B(E1:0 2 →1 1 )/B(E1:1 1 →0 1 ), and the energy ratios E 2 1 /E 0 2 and E 3 1 /E 0 2 are proposed to identify the second-order phase transition in experiments. We also found that the critical point of phase transition can be approximately described by the E(3) symmetry, which persists even for moderate N∼10 protected by the scaling behaviors of quantities at the critical point. In addition, a possible empirical example exhibiting roughly the E(3) symmetry is discussed

(E-4-Bromo-N-(2,3-dimethoxybenzylideneaniline

Directory of Open Access Journals (Sweden)

Karla Fejfarová

2010-09-01

Full Text Available The title Schiff base compound, C15H14BrNO2, was prepared by the condensation of 2,3-dimethoxybenzaldehyde with 4-bromoaniline. It adopts an E configuration with respect to the C=N bond. The dihedral angle between the two aromatic rings is 56.79 (8°. Weak C—H...O and C—-H...π bonds can be found in the crystal structure.

Search for neutralinos in e+e- reactions at the L3 experiment

International Nuclear Information System (INIS)

Starosta, R.

1992-10-01

The present thesis deals with the search for neutralinos, which are predicted in the framework of the minimal supersymmetric standard model (MSSM). The lightest of the neutralinos is favorized as the lightest supersymmetric particle. With it, how far this assumption is confirmed, all decay chains of other SUSY particles would end. The data, on which the experimental studies are based, were collected in the year 1990 with the L3 detector at the e + e - -storage ring LEP at a c.m. energy around 91 GeV. In them no hint on the existence of SUSY particles is found, whereby a) deviations of the decay width of the Z 0 boson from the standard-model prediction and b) in hadronic final states directly detectable neutralinos are looked for. The results are presented in form of regions in the parameter space of the MSSM - tan β, M 2 , μ- as well as masses for the lightest neutralinos in dependence on tan β, which are excluded with 95% c.l. Quite generally it can be stated, that a neutralino with a mass of less than 19 geV for tan β>3 is no more allowed in the framework of the MSSM. (orig.) [de

The Chang'e 3 Mission Overview

Science.gov (United States)

Li, Chunlai; Liu, Jianjun; Ren, Xin; Zuo, Wei; Tan, Xu; Wen, Weibin; Li, Han; Mu, Lingli; Su, Yan; Zhang, Hongbo; Yan, Jun; Ouyang, Ziyuan

2015-07-01

The Chang'e 3 (CE-3) mission was implemented as the first lander/rover mission of the Chinese Lunar Exploration Program (CLEP). After its successful launch at 01:30 local time on December 2, 2013, CE-3 was inserted into an eccentric polar lunar orbit on December 6, and landed to the east of a 430 m crater in northwestern Mare Imbrium (19.51°W, 44.12°N) at 21:11 on December 14, 2013. The Yutu rover separated from the lander at 04:35, December 15, and traversed for a total of 0.114 km. Acquisition of science data began during the descent of the lander and will continue for 12 months during the nominal mission. The CE-3 lander and rover each carry four science instruments. Instruments on the lander are: Landing Camera (LCAM), Terrain Camera (TCAM), Extreme Ultraviolet Camera (EUVC), and Moon-based Ultraviolet Telescope (MUVT). The four instruments on the rover are: Panoramic Camera (PCAM), VIS-NIR Imaging Spectrometer (VNIS), Active Particle induced X-ray Spectrometer (APXS), and Lunar Penetrating Radar (LPR). The science objectives of the CE-3 mission include: (1) investigation of the morphological features and geological structures of and near the landing area; (2) integrated in-situ analysis of mineral and chemical composition of and near the landing area; and (3) exploration of the terrestrial-lunar space environment and lunar-based astronomical observations. This paper describes the CE-3 objectives and measurements that address the science objectives outlined by the Comprehensive Demonstration Report of Phase II of CLEP. The CE-3 team has archived the initial science data, and we describe data accessibility by the science community.

(E,Z)-3-(3',5'-Dimethoxy-4'-hydroxy-benzylidene)-2-indolinone blocks mast cell degranulation.

Science.gov (United States)

Kiefer, S; Mertz, A C; Koryakina, A; Hamburger, M; Küenzi, P

2010-05-12

(E,Z)-3-(3',5'-Dimethoxy-4'-hydroxy-benzylidene)-2-indolinone (indolinone) is an alkaloid that has been identified as a pharmacologically active compound in extracts of the traditional anti-inflammatory herb Isatis tinctoria. Indolinone has been shown to inhibit compound 48/80-induced mast cell degranulation in vitro. Application of indolinone to bone marrow derived mast cells showed that it was uniformly distributed in the cytoplasm and that cellular uptake was terminated within minutes. Pre-treatment of IgE-sensitized mast cells with 100nM indolinone rendered them insensitive against FcvarepsilonRI-receptor dependent degranulation. However, upstream signalling induced by antigen such as activation of PI3-K and MAPK remained unaffected. We conclude that indolinone blocks mast cell degranulation at the level of granule exocitosis with an IC(50) of 54nm.

FUS-DDIT3 prevents the development of adipocytic precursors in liposarcoma by repressing PPARgamma and C/EBPalpha and activating eIF4E.

Directory of Open Access Journals (Sweden)

Pedro A Pérez-Mancera

Full Text Available BACKGROUND: FUS-DDIT3 is a chimeric protein generated by the most common chromosomal translocation t(12;16(q13;p11 linked to liposarcomas, which are characterized by the accumulation of early adipocytic precursors. Current studies indicate that FUS-DDIT3- liposarcoma develops from uncommitted progenitors. However, the precise mechanism whereby FUS-DDIT3 contributes to the differentiation arrest remains to be elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Here we have characterized the adipocyte regulatory protein network in liposarcomas of FUS-DITT3 transgenic mice and showed that PPARgamma2 and C/EBPalpha expression was altered. Consistent with in vivo data, FUS-DDIT3 MEFs and human liposarcoma cell lines showed a similar downregulation of both PPARgamma2 and C/EBPalpha expression. Complementation studies with PPARgamma but not C/EBPalpha rescued the differentiation block in committed adipocytic precursors expressing FUS-DDIT3. Our results further show that FUS-DDIT3 interferes with the control of initiation of translation by upregulation of the eukaryotic translation initiation factors eIF2 and eIF4E both in FUS-DDIT3 mice and human liposarcomas cell lines, explaining the shift towards the truncated p30 isoform of C/EBPalpha in liposarcomas. Suppression of the FUS-DDIT3 transgene did rescue this adipocyte differentiation block. Moreover, eIF4E was also strongly upregulated in normal adipose tissue of FUS-DDIT3 transgenic mice, suggesting that overexpression of eIF4E may be a primary event in the initiation of liposarcomas. Reporter assays showed FUS-DDIT3 is involved in the upregulation of eIF4E in liposarcomas and that both domains of the fusion protein are required for affecting eIF4E expression. CONCLUSIONS/SIGNIFICANCE: Taken together, this study provides evidence of the molecular mechanisms involve in the disruption of normal adipocyte differentiation program in liposarcoma harbouring the chimeric gene FUS-DDIT3.

3D-e-Chem-VM: Structural Cheminformatics Research Infrastructure in a Freely Available Virtual Machine

NARCIS (Netherlands)

McGuire, R.; Verhoeven, S.; Vass, M.; Vriend, G.; Esch, I.J. de; Lusher, S.J.; Leurs, R.; Ridder, L.; Kooistra, A.J.; Ritschel, T.; Graaf, C. de

2017-01-01

3D-e-Chem-VM is an open source, freely available Virtual Machine ( http://3d-e-chem.github.io/3D-e-Chem-VM/ ) that integrates cheminformatics and bioinformatics tools for the analysis of protein-ligand interaction data. 3D-e-Chem-VM consists of software libraries, and database and workflow tools

3D-e-Chem-VM : Structural Cheminformatics Research Infrastructure in a Freely Available Virtual Machine

NARCIS (Netherlands)

McGuire, Ross; Verhoeven, Stefan; Vass, Márton; Vriend, Gerrit; De Esch, Iwan J P; Lusher, Scott J.; Leurs, Rob; Ridder, Lars; Kooistra, Albert J.; Ritschel, Tina; de Graaf, C.

2017-01-01

3D-e-Chem-VM is an open source, freely available Virtual Machine ( http://3d-e-chem.github.io/3D-e-Chem-VM/ ) that integrates cheminformatics and bioinformatics tools for the analysis of protein-ligand interaction data. 3D-e-Chem-VM consists of software libraries, and database and workflow tools

Precise measurement of the neutron magnetic form factor from quasielastic 3 rvec He(rvec e,e')

International Nuclear Information System (INIS)

Gao, H.

1997-01-01

Polarized 3 He targets have proven to be a useful tool for studying the electric and magnetic form factors of the neutron, and the spin structure of the neutron. The neutron magnetic form factor at low Q 2 was determined previously at MIT-Bates from the quasielastic 3 rvec He(rvec e, e ' ) process. New experiment was planned at TJNAF to systematically measure the inclusive 3 He quasielastic transverse asymmetry, A T ' , at Q 2 = 0.1 - 0.5 (GeV/c) 2 with high statistical and systematic accuracy. A 2% statistical uncertainty is aimed at all the pro- posed values of Q 2 , and 3% systematic uncertainty for A T ' can be achieved for this experiment. The precise data will constrain theoretical calculations of 3 He quasielastic asymmetry. Furthermore, the neutron magnetic form factor at Q 2 = 0.1 - 0.5 (GeV/c) 2 will be extracted from the measured asymmetries with an overall uncertainty of 2%. Precise measurements of G n M at low Q 2 will resolve the discrepancy among the existing data in the same Q 2 region

Pyrosequencing-Based Assays for Rapid Detection of HER2 and HER3 Mutations in Clinical Samples Uncover an E332E Mutation Affecting HER3 in Retroperitoneal Leiomyosarcoma

Directory of Open Access Journals (Sweden)

Paula González-Alonso

2015-08-01

Full Text Available Mutations in Human Epidermal Growth Factor Receptors (HER are associated with poor prognosis of several types of solid tumors. Although HER-mutation detection methods are currently available, such as Next-Generation Sequencing (NGS, alternative pyrosequencing allow the rapid characterization of specific mutations. We developed specific PCR-based pyrosequencing assays for identification of most prevalent HER2 and HER3 mutations, including S310F/Y, R678Q, L755M/P/S/W, V777A/L/M, 774-776 insertion, and V842I mutations in HER2, as well as M91I, V104M/L, D297N/V/Y, and E332E/K mutations in HER3. We tested 85 Formalin Fixed and Paraffin Embbeded (FFPE samples and we detected three HER2-V842I mutations in colorectal carcinoma (CRC, ovarian carcinoma, and pancreatic carcinoma patients, respectively, and a HER2-L755M mutation in a CRC specimen. We also determined the presence of a HER3-E332K mutation in an urothelial carcinoma sample, and two HER3-D297Y mutations, in both gastric adenocarcinoma and CRC specimens. The D297Y mutation was previously detected in breast and gastric tumors, but not in CRC. Moreover, we found a not-previously-described HER3-E332E synonymous mutation in a retroperitoneal leiomyosarcoma patient. The pyrosequencing assays presented here allow the detection and characterization of specific HER2 and HER3 mutations. These pyrosequencing assays might be implemented in routine diagnosis for molecular characterization of HER2/HER3 receptors as an alternative to complex NGS approaches.

(E-3-(4-Bromophenyl-1-(3,4-dichlorophenylprop-2-en-1-one

Directory of Open Access Journals (Sweden)

Rajni Kant

2009-04-01

Full Text Available The molecule of the title compound, C15H9BrCl2O, is shown to be the E isomer, with the 3,4-dichlorobenzoyl and p-bromophenyl substituents in trans positions with respect to the chalcone olefin bond. The molecule is non-planar, the two aromatic rings forming a dihedral angle of 49.58 (1°.

M3D (Media 3D): a new programming language for web-based virtual reality in E-Learning and Edutainment

Science.gov (United States)

Chakaveh, Sepideh; Skaley, Detlef; Laine, Patricia; Haeger, Ralf; Maad, Soha

2003-01-01

Today, interactive multimedia educational systems are well established, as they prove useful instruments to enhance one's learning capabilities. Hitherto, the main difficulty with almost all E-Learning systems was latent in the rich media implementation techniques. This meant that each and every system should be created individually as reapplying the media, be it only a part, or the whole content was not directly possible, as everything must be applied mechanically i.e. by hand. Consequently making E-learning systems exceedingly expensive to generate, both in time and money terms. Media-3D or M3D is a new platform independent programming language, developed at the Fraunhofer Institute Media Communication to enable visualisation and simulation of E-Learning multimedia content. M3D is an XML-based language, which is capable of distinguishing between the3D models from that of the 3D scenes, as well as handling provisions for animations, within the programme. Here we give a technical account of M3D programming language and briefly describe two specific application scenarios where M3D is applied to create virtual reality E-Learning content for training of technical personnel.

NIR Color vs Launch Date: A 20-Year Analysis of Space Weathering Effects on the Boeing 376 Spacecraft

Science.gov (United States)

Frith, James; Anz-Meador, Philip; Lederer, Sue; Cowardin, Heather; Buckalew, Brent

2015-01-01

The Boeing HS-376 spin stabilized spacecraft was a popular design that was launched continuously into geosynchronous orbit starting in 1980 with the last launch occurring in 2002. Over 50 of the HS-376 buses were produced to fulfill a variety of different communication missions for countries all over the world. The design of the bus is easily approximated as a telescoping cylinder that is covered with solar cells and an Earth facing antenna that is despun at the top of the cylinder. The similarity in design and the number of spacecraft launched over a long period of time make the HS-376 a prime target for studying the effects of solar weathering on solar panels as a function of time. A selection of primarily non-operational HS-376 spacecraft launched over a 20 year time period were observed using the United Kingdom Infrared Telescope on Mauna Kea and multi-band near-infrared photometry produced. Each spacecraft was observed for an entire night cycling through ZYJHK filters and time-varying colors produced to compare near-infrared color as a function of launch date. The resulting analysis shown here may help in the future to set launch date constraints on the parent object of unidentified debris objects or other unknown spacecraft.

Procedure Redesign Methods : E3-Control: a redesign methodology for control procedures

NARCIS (Netherlands)

Liu, J.; Hofman, W.J.; Tan, Y.H.

2011-01-01

This chapter highlights the core research methodology, e3-control, that is applied throughout the ITAIDE project for the purpose of control procedure redesign. We present the key concept of the e3-control methodology and its technical guidelines. Based on the output of this chapter, domain experts

Functional expression of 5-HT{sub 2A} receptor in osteoblastic MC3T3-E1 cells

Energy Technology Data Exchange (ETDEWEB)

Hirai, Takao; Kaneshige, Kota; Kurosaki, Teruko [Department of Molecular Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Gakuen-cho, Fukuyama, Hiroshima 729-0292 (Japan); Nishio, Hiroaki, E-mail: nishio@fupharm.fukuyama-u.ac.jp [Department of Molecular Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Gakuen-cho, Fukuyama, Hiroshima 729-0292 (Japan)

2010-05-28

In the previous study, we reported the gene expression for proteins related to the function of 5-hydroxytryptamine (5-HT, serotonin) and elucidated the expression patterns of 5-HT{sub 2} receptor subtypes in mouse osteoblasts. In the present study, we evaluated the possible involvement of 5-HT receptor subtypes and its inactivation system in MC3T3-E1 cells, an osteoblast cell line. DOI, a 5-HT{sub 2A} and 5-HT{sub 2C} receptor selective agonist, as well as 5-HT concentration-dependently increased proliferative activities of MC3T3-E1 cells in their premature period. This effect of 5-HT on cell proliferation were inhibited by ketanserin, a 5-HT{sub 2A} receptor specific antagonist. Moreover, both DOI-induced cell proliferation and phosphorylation of ERK1 and 2 proteins were inhibited by PD98059 and U0126, selective inhibitors of MEK in a concentration-dependent manner. Furthermore, treatment with fluoxetine, a 5-HT specific re-uptake inhibitor which inactivate the function of extracellular 5-HT, significantly increased the proliferative activities of MC3T3-E1 cells in a concentration-dependent manner. Our data indicate that 5-HT fill the role for proliferation of osteoblast cells in their premature period. Notably, 5-HT{sub 2A} receptor may be functionally expressed to regulate mechanisms underlying osteoblast cell proliferation, at least in part, through activation of ERK/MAPK pathways in MC3T3-E1 cells.

GPM GROUND VALIDATION NCAR CLOUD MICROPHYSICS PARTICLE PROBES MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validation NCAR Cloud Microphysics Particle Probes MC3E dataset was collected during the Midlatitude Continental Convective Clouds Experiment (MC3E),...

Enhancing osteogenic differentiation of MC3T3-E1 cells by immobilizing RGD onto liquid crystal substrate

International Nuclear Information System (INIS)

Wu, Shaopeng; Yang, Xiaohui; Li, Wenqiang; Du, Lin; Zeng, Rong; Tu, Mei

2017-01-01

To understand the effects of GRGDF modification on MC3T3-E1 cell behavior, we cultured these cells onto a biomimetic liquid crystalline matrix modified with GRGDF peptide (OPC-GA-RGD). Successful immobilization of GRGDF on the liquid crystalline surface was verified by fluorescent labeling, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). OPC-GA-RGDs retained its liquid crystalline feature after surface modification. The RGD-immobilized OPC substrate was hardly beneficial to initial cell adhesion but could support long-term cell survival. The enhancement in cell proliferation did not correlate with RGD density. The lower GRGDF density immobilized on the liquid crystalline OPC matrix (OPC-GA-RGD3) promoted cell adhesion, proliferation, ALP expression level and mineralization, suggesting that both the viscoelasticity-based mechanical stimuli and receptor/ligand-based biochemical cue synergistically modulate MC3T3-E1 cell behavior. - Highlight: • A novel type of GRGDF-immobilized liquid crystalline matrices was fabricated and served as a substrate for the in vitro culture of MC3T3-E1 cells. • The lower RGD density might provide a better condition for initial cell adhesion and proliferation, up-regulation of ALP expression levels, and mineralization. • The intrinsic liquid crystalline feature of OPC matrix, instead of RGD efficiency, promoted initial cell adhesion. • Properties of the liquid crystalline OPC matrix together with the stable receptor-ligand binging synergistically modulated MC3T3-E1 cell behavior.

Enhancing osteogenic differentiation of MC3T3-E1 cells by immobilizing RGD onto liquid crystal substrate

Energy Technology Data Exchange (ETDEWEB)

Wu, Shaopeng; Yang, Xiaohui; Li, Wenqiang; Du, Lin; Zeng, Rong; Tu, Mei, E-mail: tumei@jnu.edu.cn

2017-02-01

To understand the effects of GRGDF modification on MC3T3-E1 cell behavior, we cultured these cells onto a biomimetic liquid crystalline matrix modified with GRGDF peptide (OPC-GA-RGD). Successful immobilization of GRGDF on the liquid crystalline surface was verified by fluorescent labeling, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). OPC-GA-RGDs retained its liquid crystalline feature after surface modification. The RGD-immobilized OPC substrate was hardly beneficial to initial cell adhesion but could support long-term cell survival. The enhancement in cell proliferation did not correlate with RGD density. The lower GRGDF density immobilized on the liquid crystalline OPC matrix (OPC-GA-RGD3) promoted cell adhesion, proliferation, ALP expression level and mineralization, suggesting that both the viscoelasticity-based mechanical stimuli and receptor/ligand-based biochemical cue synergistically modulate MC3T3-E1 cell behavior. - Highlight: • A novel type of GRGDF-immobilized liquid crystalline matrices was fabricated and served as a substrate for the in vitro culture of MC3T3-E1 cells. • The lower RGD density might provide a better condition for initial cell adhesion and proliferation, up-regulation of ALP expression levels, and mineralization. • The intrinsic liquid crystalline feature of OPC matrix, instead of RGD efficiency, promoted initial cell adhesion. • Properties of the liquid crystalline OPC matrix together with the stable receptor-ligand binging synergistically modulated MC3T3-E1 cell behavior.

Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer

Czech Academy of Sciences Publication Activity Database

Wagner, Susan; Herrmannová, Anna; Šikrová, Darina; Valášek, Leoš Shivaya

2016-01-01

Roč. 44, č. 22 (2016), s. 10772-10788 ISSN 0305-1048 R&D Projects: GA ČR(CZ) GA14-05394S EU Projects: Wellcome Trust(GB) 090812/B/09/A Institutional support: RVO:61388971 Keywords : Human eIF3b * nucleation core * yeast -like core Subject RIV: EE - Microbiology, Virology Impact factor: 10.162, year: 2016

eLearning and eMaking: 3D Printing Blurring the Digital and the Physical

Directory of Open Access Journals (Sweden)

Jennifer Loy

2014-02-01

Full Text Available This article considers the potential of 3D printing as an eLearning tool for design education and the role of eMaking in bringing together the virtual and the physical in the design studio. eLearning has matured from the basics of lecture capture into sophisticated, interactive learning activities for students. At the same time, laptops and internet enabled phones have made computer-based learning mobile, invading classroom learning, changing communication between students, enabling on the spot research, and making the recording of ideas and activities easier. The barriers between online and offline are becoming blurred in a combined digital and physical learning environment. Three-dimensional printing is part of this unification and can be an empowering learning tool for students, changing their relationship with the virtual and the physical, allowing them to take ideas and thinking from screen to reality and back again in an iterative, connected process, however, from an eLearning point of view it is, more importantly, a transformative technology with the potential to change the relationship of the learner to their learning and the scope and nature of their work. Examples from Griffith Product Design student learning illustrate the potential of eMaking to enhance combined learning in a digital age.

Using Email to Enable E[superscript 3] (Effective, Efficient, and Engaging) Learning

Science.gov (United States)

Kim, ChanMin

2008-01-01

This article argues that technology that supports both noncognitive and cognitive aspects can make learning more effective, efficient, and engaging (e[superscript 3]-learning). The technology of interest in this article is email. The investigation focuses on characteristics of email that are likely to enable e[superscript 3]-learning. In addition,…

Characterization of polar organosulfates in secondary organic aerosol from the unsaturated aldehydes 2-E-pentenal, 2-E-hexenal, and 3-Z-hexenal

Directory of Open Access Journals (Sweden)

M. S. Shalamzari

2016-06-01

Full Text Available We show in the present study that the unsaturated aldehydes 2-E-pentenal, 2-E-hexenal, and 3-Z-hexenal are biogenic volatile organic compound (BVOC precursors for polar organosulfates with molecular weights (MWs 230 and 214, which are also present in ambient fine aerosol from a forested site, i.e., K-puszta, Hungary. These results complement those obtained in a previous study showing that the green leaf aldehyde 3-Z-hexenal serves as a precursor for MW 226 organosulfates. Thus, in addition to isoprene, the green leaf volatiles (GLVs 2-E-hexenal and 3-Z-hexenal, emitted due to plant stress (mechanical wounding or insect attack, and 2-E-pentenal, a photolysis product of 3-Z-hexenal, should be taken into account for secondary organic aerosol and organosulfate formation. Polar organosulfates are of climatic relevance because of their hydrophilic properties and cloud effects. Extensive use was made of organic mass spectrometry (MS and detailed interpretation of MS data (i.e., ion trap MS and accurate mass measurements to elucidate the chemical structures of the MW 230, 214 and 170 organosulfates formed from 2-E-pentenal and indirectly from 2-E-hexenal and 3-Z-hexenal. In addition, quantum chemical calculations were performed to explain the different mass spectral behavior of 2,3-dihydroxypentanoic acid sulfate derivatives, where only the isomer with the sulfate group at C-3 results in the loss of SO3. The MW 214 organosulfates formed from 2-E-pentenal are explained by epoxidation of the double bond in the gas phase and sulfation of the epoxy group with sulfuric acid in the particle phase through the same pathway as that proposed for 3-sulfooxy-2-hydroxy-2-methylpropanoic acid from the isoprene-related α,β-unsaturated aldehyde methacrolein in previous work (Lin et al., 2013. The MW 230 organosulfates formed from 2-E-pentenal are tentatively explained by a novel pathway, which bears features of the latter pathway but introduces an additional hydroxyl

L'evoluzione della stampa 3D e le sue applicazioni in campo museale

Directory of Open Access Journals (Sweden)

Francesco Pignatelli

2014-07-01

Full Text Available ItLa stampa 3D è passata dall'uso solo industriale al grande pubblico degli appassionati e dei maker. Questa evoluzione interessa anche i musei, che possono adottare le tecniche di stampa 3D per rendere più fruibili le loro collezioni, creare una maggiore interattività con il pubblico potenziale e generare nuovo business attraverso il merchandising. Più in futuro i musei andranno verso un modello in cui l'originale e la copia ad alta precisione ottenuta con la stampa 3D, e persino il suo modello digitale, avranno ruoli complementari.En3D printing migrated from industrial manufacturing to a much wider audience of small companies and self-producers. This evolution shows interesting implications also for museums: they can adopt 3D printing techniques to make their collections more accessible, develop more interactivity for their visitors and generate new business through a new kind of merchandising. More will change in the future, when the original artwork and its 3D-printed replicas, and even its digital 3D model, will play complementary roles. 

GA3 e Paclobutrazol no florescimento e na produção de frutos em duas cultivares de morangueiro GA3 and Paclobutrazol on the flowering and yield of strawberry

Directory of Open Access Journals (Sweden)

Jaime Duarte Filho

2004-06-01

Full Text Available Neste trabalho estudou-se o efeito dos fitorreguladores Ga3 e Paclobutrazol no florescimento e produção de frutos de morangueiro (Fragaria x ananassa Duch.. O experimento foi conduzido em Caldas (MG, na EPAMIG. O delineamento experimental adotado foi o de blocos casualizados, com quatro repetições, num esquema fatorial 2x4, onde as variáveis dependentes foram duas cultivares ('Oso Grande' e 'Seascape' e os tratamentos controle (água; GA3 a 40 mg L-1 em duas aplicações (aos 45 e 65 dias após o transplante; paclobutrazol (produto comercial Cultar 10% a 100 mg L-1 aos 75 dias após o transplante das mudas; combinação de GA3 + paclobutrazol aplicados aos 45; 65 e aos 75 dias, respectivamente, após o transplante. Os tratamentos foram aplicados via foliar em ambas as cultivares. A resposta à aplicação dos fitorreguladores GA3 e paclobutrazol em morangueiro depende da cultivar. A cv. Seascape, de dia neutro, respondeu melhor à aplicação de GA3 que a 'Oso Grande', de dia curto; Por outro lado, o paclobutrazol, quando aplicado via foliar, aos 75 dias após o transplante, reduziu o florescimento do morangueiro o que mostra a necessidade de mais pesquisas sobre dosagens e épocas de aplicação do paclobutrazol nesta cultura.The effect of bioregulators GA3 and paclobutrazol was evaluated on flowering and yield of strawberry cultivars Oso Grande and Seascape. The experiment was carried out in Caldas, Minas Gerais State, Brazil. The treatments consisted of control (no application of bioregulators; GA3 at 40 mg L-1, in two applications (45 and 65 days after transplanting date; paclobutrazol (commercial product Cultar, 10% at 100 mg L-1, 75 days after transplanting date; combination of GA3 + paclobutrazol, applied 45; 65 and 75 days, respectively, after transplanting date. The experimental design was a randomized complete block, with four replications, in a factorial 2x4. The response to the bioregulators GA3 and Paclobutrazol was cultivar

Indirubin-3′-(2,3 dihydroxypropyl)-oximether (E804) is a potent modulator of LPS-stimulated macrophage functions

Energy Technology Data Exchange (ETDEWEB)

Babcock, Abigail S. [Department of Biological Sciences, Clemson University, Clemson, SC 29634 (United States); Anderson, Amy L. [Department of Biological Sciences, Clemson University, Clemson, SC 29634 (United States); Graduate Program in Environmental Toxicology, Clemson University, Clemson, SC 29634 (United States); Rice, Charles D., E-mail: cdrice@clemson.edu [Department of Biological Sciences, Clemson University, Clemson, SC 29634 (United States); Graduate Program in Environmental Toxicology, Clemson University, Clemson, SC 29634 (United States)

2013-01-01

Indirubin is a deep-red bis-indole isomer of indigo blue, both of which are biologically active ingredients in Danggui Longhui Wan, an ancient Chinese herbal tea mixture used to treat neoplasia and chronic inflammation and to enhance detoxification of xenobiotics. Multiple indirubin derivatives have been synthesized and shown to inhibit cyclin-dependent kinases (CDKs) and glycogen-synthase kinase (GSK-3β) with varying degrees of potency. Several indirubins are also aryl hydrocarbon receptor (AhR) agonists, with AhR-associated activities covering a wide range of potencies, depending on molecular structure. This study examined the effects of indirubin-3′-(2,3 dihydroxypropyl)-oximether (E804), a novel indirubin with potent STAT3 inhibitory properties, on basal and LPS-inducible activities in murine RAW264.7 macrophages. Using a focused commercial qRT-PCR array platform (SuperArray®), the effects of E804 on expression of a suite of genes associated with stress and toxicity were determined. Most genes up-regulated by LPS treatment were suppressed by E804; including LPS-induced expression of pro-inflammatory cytokines and receptors, apoptosis control genes, and oxidative stress response genes. Using qRT-PCR as a follow up to the commercial arrays, E804 treatment suppressed LPS-induced COX-2, iNOS, IL-6 and IL-10 gene expression, though the effects on iNOS and COX-2 protein expression were less dramatic. E804 also inhibited LPS-induced secretion of IL-6 and IL-10. Functional endpoints, including iNOS and lysozyme enzymatic activity, phagocytosis of fluorescent latex beads, and intracellular killing of bacteria, were also examined, and in each experimental condition E804 suppressed activities. Collectively, these results indicate that E804 is a potent modulator of pro-inflammatory profiles in LPS-treated macrophages. -- Highlights: ► RAW 264.7 macrophages were treated with 1 μM Indirubin E804, 1 μg/ml LPS, or both. ► E804 suppresses LPS-induced expression of i

Track based alignment of the Mu3e detector

Energy Technology Data Exchange (ETDEWEB)

Hartenstein, Ulrich [Institut fuer Kernphysik, Universitaet Mainz (Germany)

2016-07-01

The Mu3e experiment searches for the lepton flavor violating decay μ{sup +} → e{sup +}e{sup -}e{sup +} with a sensitivity goal for the branching fraction of better than 10{sup -16}. This process is heavily supressed in the standard model of particle physics (BR < 10{sup -50}) which makes an observation of this decay a clear indication of new physics. For track reconstruction, four barrel shaped layers consisting of about 3000 high-voltage monolithic active pixel sensors (HV-MAPS) are used. The position, orientation and possible deformations of these sensors must be known to greater precision than the assembly tolerances. A track based alignment via the General Broken Lines fit and the Millepede-II algorithm will be used to achieve this precision in the final detector. The talk discusses a study of the required alignment precision and preparations for aligning the detector using a detailed simulation.

Calibration of a plastic detector PVT of large dimensions for the detection gamma radiation

International Nuclear Information System (INIS)

Armenteros Carmona, J. C.; Suarez navarro, M. J.; Gonzalez-gonzalez, J. A.; Pujol, L.

2011-01-01

This article describes the development work on the porch Rad Sentry Canberra brand that uses 2 plastic scintillation detectors PVT large (172 x 32 x 4 cm). In this paper we present the different calibration necessary for the proper functioning of equipment: channel-energy calibration, energy resolution, and efficiency-energy.

Structural Glass Beams with Embedded Glass Fibre Reinforcement

NARCIS (Netherlands)

Louter, P.C.; Leung, Calvin; Kolstein, M.H.; Vambersky, J.N.J.A.; Bos, Freek; Louter, Pieter Christiaan; Veer, Fred

2010-01-01

This paper investigates the possibilities of pultruded glass fibre rods as embedded reinforcement in SentryGlas (SG) laminated glass beams. To do so, a series of pullout tests, to investigate the bond strength of the rods to the laminate, and a series of beam tests, to investigate the post-breakage

Characterizations of Space Curves According to Bishop Darboux Vector in Euclidean 3-Space E3

OpenAIRE

Huseyin KOCAYIGIT; Ali OZDEMIR

2014-01-01

In this paper, we obtained some characterizations of space curves according to Bihop frame in Euclidean 3-space E3 by using Laplacian operator and Levi-Civita connection. Furthermore, we gave the general differential equations which characterize the space curves according to the Bishop Darboux vector and the normal Bishop Darboux vector.

Superfluid H3e in globally isotropic random media

Science.gov (United States)

Ikeda, Ryusuke; Aoyama, Kazushi

2009-02-01

Recent theoretical and experimental studies of superfluid H3e in aerogels with a global anisotropy created, e.g., by an external stress have definitely shown that the A -like phase with an equal-spin pairing in such aerogel samples is in the Anderson-Brinkman-Morel (ABM) (or axial) pairing state. In this paper, the A -like phase of superfluid H3e in globally isotropic aerogel is studied in detail by assuming a weakly disordered system in which singular topological defects are absent. Through calculation of the free energy, a disordered ABM state is found to be the best candidate of the pairing state of the globally isotropic A -like phase. Further, it is found through a one-loop renormalization-group calculation that the coreless continuous vortices (or vortex-Skyrmions) are irrelevant to the long-distance behavior of disorder-induced textures, and that the superfluidity is maintained in spite of lack of the conventional superfluid long-range order. Therefore, the globally isotropic A -like phase at weak disorder is, like in the case with a globally stretched anisotropy, a glass phase with the ABM pairing and shows superfluidity.

Subsonic Ultra Green Aircraft Research

Science.gov (United States)

Bradley, Marty K.; Droney, Christopher K.

2011-01-01

This Final Report summarizes the work accomplished by the Boeing Subsonic Ultra Green Aircraft Research (SUGAR) team in Phase 1, which includes the time period of October 2008 through March 2010. The team consisted of Boeing Research and Technology, Boeing Commercial Airplanes, General Electric, and Georgia Tech. The team completed the development of a comprehensive future scenario for world-wide commercial aviation, selected baseline and advanced configurations for detailed study, generated technology suites for each configuration, conducted detailed performance analysis, calculated noise and emissions, assessed technology risks, and developed technology roadmaps. Five concepts were evaluated in detail: 2008 baseline, N+3 reference, N+3 high span strut braced wing, N+3 gas turbine battery electric concept, and N+3 hybrid wing body. A wide portfolio of technologies was identified to address the NASA N+3 goals. Significant improvements in air traffic management, aerodynamics, materials and structures, aircraft systems, propulsion, and acoustics are needed. Recommendations for Phase 2 concept and technology projects have been identified.

Hepatitis E Virus Genotype 3 in Humans and Swine, Bolivia

Science.gov (United States)

Cavallo, Annalisa; Gonzales, José Luis; Bonelli, Sara Irene; Valda, Ybar; Pieri, Angela; Segundo, Higinio; Ibañez, Ramón; Mantella, Antonia; Bartalesi, Filippo; Tolari, Francesco; Bartoloni, Alessandro

2011-01-01

We determined the seroprevalence of hepatitis E virus (HEV) in persons in 2 rural communities in southeastern Bolivia and the presence of HEV in human and swine fecal samples. HEV seroprevalence was 6.3%, and HEV genotype 3 strains with high sequence homology were detected. PMID:21801630

Monitoring of high-risk pregnancies using E3 and HPL

Energy Technology Data Exchange (ETDEWEB)

Bieglmayer, Ch; Spona, J

1981-01-01

Routine determinations of HPL and E3 together with other clinical and laboratory checks offer usable information on the state of pregnancy and assist the doctor in taking decisions on the use of therapeutical measures. The anamnesis, the presence of placental insufficiency of EPH-gestosis are important indications for monitoring the pregnancy using HPL and E3. A commercial RIA apparatus (Centria 2) allowing the determination of HPL in a mere hour was compared with our established laboratory method. In 140 determinations, the correlation was r=0.75. Considering its capacity and method, the instrument seems to be more suitable for a small specialized laboratory.

miR-195 inhibited abnormal activation of osteoblast differentiation in MC3T3-E1 cells via targeting RAF-1.

Science.gov (United States)

Chao, Chen; Li, Feng; Tan, Zhiping; Zhang, Weizhi; Yang, Yifeng; Luo, Cheng

2018-01-15

Recent reports have demonstrated that RAF-1 L613V (a mutant of RAF-1) mutant mice show bone deformities similar to Noonan syndrome. It has been suggested that RAF-1 L613V might abnormally activate osteoblast differentiation of MC3T3-E1 cells. To demonstrate that RAF-1 is associated with bone deformity and that RAF-1 L613V dependent bone deformity could be inhibited by microRNA-195 (miR-195), we first investigated the amplifying influence of wild-type RAF-1 (WT) or RAF-1 L613V (L613V) on the viability and differentiation of MC3T3-E1 cells induced by bone morphogenetic protein-2 (BMP-2) via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis. Subsequently, we investigated the blocking effect and its mechanism of miR-195 for abnormal activation of osteoblast differentiation of MC3T3-E1 cells via targeting RAF-1. RAF-1, especially RAF-1 L613V , abnormally activates osteoblast differentiation of MC3T3-E1 cells induced by BMP-2. Meanwhile, miR-195 could inhibit the cell viability and differentiation of MC3T3-E1 cells. Transfection of miR-195 largely suppressed the L613V-induced viability and osteoblast differentiation of MC3T3-E1 cells and attenuated the accelerative effect of L613V on runt-related transcription factor-2 (Runx2), Osterix (OSX), alkaline phosphatase (ALP), osteocalcin (OCN), and distal-less homeobox 5 (DLX5) osteogenic gene expressions. In addition, miR-195 decreased the expression of RAF-1 mRNA and protein by directly targeting the 3'-untranslated regions (3'-UTR) of RAF-1 mRNA in MC3T3-E1 cells. Our findings indicated that miR-195 inhibited WT and L613V RAF-1 induced hyperactive osteoblast differentiation in MC3T3-E1 cells by targeting RAF-1. miR-195 might be a novel therapeutic agent for the treatment of L613V-induced bone deformity in Noonan syndrome. Copyright © 2017. Published by

Competencia Digital: Uso y manejo de modelos 3D tridimensionales digitales e impresos en 3D

Directory of Open Access Journals (Sweden)

Jose Luis Saorín

2017-07-01

Full Text Available El uso y manejo de modelos tridimensionales digitales no está concebido dentro de la competencia digital de los currículos de secundaria y Bachillerato. Sin embargo muchos autores relacionan la competencia digital con el manejo de modelos 3D, el modelado 3D y entornos virtuales tridimensionales (Realidad aumentada, virtual,…. En este artículo se presenta un recurso educativo para facilitar el acceso a contenidos didácticos de carácter tridimensional digital y tangible. Determinadas materias precisan de la comprensión e interpretación de conceptos volumétricos: los recursos didácticos innovadores para la edición, visualización e impresión 3D ofrecen una alternativa a las representaciones 2D en los procesos de enseñanza y aprendizaje. En este artículo se describe la creación de un catálogo escultórico que contempla versiones digitales y tangibles de modelos tridimensionales de las esculturas a través de tecnologías innovadoras de bajo coste como la visualización e impresión 3D. La prueba piloto desarrollada con 15 alumnos de bachillerato recoge una alta valoración de los participantes sobre las tecnologías empleadas.

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.

Science.gov (United States)

Brown, Nicholas G; VanderLinden, Ryan; Watson, Edmond R; Weissmann, Florian; Ordureau, Alban; Wu, Kuen-Phon; Zhang, Wei; Yu, Shanshan; Mercredi, Peter Y; Harrison, Joseph S; Davidson, Iain F; Qiao, Renping; Lu, Ying; Dube, Prakash; Brunner, Michael R; Grace, Christy R R; Miller, Darcie J; Haselbach, David; Jarvis, Marc A; Yamaguchi, Masaya; Yanishevski, David; Petzold, Georg; Sidhu, Sachdev S; Kuhlman, Brian; Kirschner, Marc W; Harper, J Wade; Peters, Jan-Michael; Stark, Holger; Schulman, Brenda A

2016-06-02

Protein ubiquitination involves E1, E2, and E3 trienzyme cascades. E2 and RING E3 enzymes often collaborate to first prime a substrate with a single ubiquitin (UB) and then achieve different forms of polyubiquitination: multiubiquitination of several sites and elongation of linkage-specific UB chains. Here, cryo-EM and biochemistry show that the human E3 anaphase-promoting complex/cyclosome (APC/C) and its two partner E2s, UBE2C (aka UBCH10) and UBE2S, adopt specialized catalytic architectures for these two distinct forms of polyubiquitination. The APC/C RING constrains UBE2C proximal to a substrate and simultaneously binds a substrate-linked UB to drive processive multiubiquitination. Alternatively, during UB chain elongation, the RING does not bind UBE2S but rather lures an evolving substrate-linked UB to UBE2S positioned through a cullin interaction to generate a Lys11-linked chain. Our findings define mechanisms of APC/C regulation, and establish principles by which specialized E3-E2-substrate-UB architectures control different forms of polyubiquitination. Copyright © 2016 Elsevier Inc. All rights reserved.

Expressão da CASPASE-3 e CD-34 no adenocarcinoma de próstata

Directory of Open Access Journals (Sweden)

Vicente Paulo da Motta

Full Text Available OBJETIVO: 1. Avaliar em qual percentual as células tumorais se marcam com caspase-3 e CD-34; 2. quantificá-los nas células tumorais; 3. verificar correlação entre quantificação e grau de malignidade tumoral; 4. correlacioná-los entre si. MÉTODOS: Estudaram-se 38 blocos com adenocarcinoma, classificados por Gleason e marcação imunoistoquímica para caspase-3 e CD-34. As proteínas imunomarcadas foram quantificadas no software Immuno do Sistema Samba 4000 de citofotometria de imagem, pelo índice de marcagem e densidade óptica. RESULTADOS: Imunomarcou-se 25 lâminas para caspase-3 e 34 para CD-34. As quantificações da caspase-3 para o índice de marcagem foram acima de 50 em 76% e, para densidade óptica, abaixo de 50 para 96%. Em relação ao CD-34, índice de marcagem foi acima de 50 em 59% e densidade óptica abaixo de 50 em 56%. As correlações entre expressões dos marcadores e a gravidade do tumor, assim como entre os marcadores, não evidenciaram significância estatística. Não se mostrou relação de expressão com o score de Gleason. CONCLUSÃO: A presença caspase-3 e CD-34 foi de 73,5% e 100%, respectivamente; 2. caspase-3 e CD-34 apresentaram alta expressão do índice de marcagem, e baixa para densidade óptica; 3. não houve correlação entre as quantificações com a classificação de Gleason; 4. não houve correlação das expressões dos dois marcadores entre si.

G2E3 is a nucleo-cytoplasmic shuttling protein with DNA damage responsive localization

International Nuclear Information System (INIS)

Brooks, William S.; Banerjee, Sami; Crawford, David F.

2007-01-01

G2E3 was originally described as a G2/M-specific gene with DNA damage responsive expression. The presence of a conserved HECT domain within the carboxy-terminus of the protein indicated that it likely functions as a ubiquitin ligase or E3. Although HECT domains are known to function in this capacity for many proteins, we demonstrate that a portion of the HECT domain from G2E3 plays an important role in the dynamic subcellular localization of the protein. We have shown that G2E3 is a nucleo-cytoplasmic shuttling protein with nuclear export mediated by a novel nuclear export domain that functions independently of CRM1. In full-length G2E3, a separate region of the HECT domain suppresses the function of the NES. Additionally, G2E3 contains a nucleolar localization signal (NoLS) in its amino terminus. Localization of G2E3 to the nucleolus is a dynamic process, and the protein delocalizes from the nucleolus rapidly after DNA damage. Cell cycle phase-specific expression and highly regulated subcellular localization of G2E3 suggest a possible role in cell cycle regulation and the cellular response to DNA damage

Validation of an e-Learning 3.0 Critical Success Factors Framework: A Qualitative Research

Directory of Open Access Journals (Sweden)

Paula Miranda

2017-09-01

Full Text Available Aim/Purpose: As e-Learning 3.0 evolves from a theoretical construct into an actual solution for online learning, it becomes crucial to accompany this progress by scrutinising the elements that are at the origin of its success. Background: This paper outlines a framework of e-Learning 3.0’s critical success factors and its empirical validation. Methodology: The framework is the result of an extensive literature review and its empirical substantiation derives from semi-structured interviews with e-Learning experts. Contribution: The viewpoints of the experts enable the confirmation and the refinement of the original framework and serve as a foundation for the prospective implementation of e-Learning 3.0. Findings: The analysis of the interviews demonstrates that e-Learning 3.0 remains in its early stages with a reticent dissemination. Nonetheless, the interviewees invoked factors related to technology, content and stakeholders as being critical for the success of this new phase of e-Learning. Recommendations for Practitioners: Practitioners can use the framework as a guide for promoting and implementing effective e-Learning 3.0 initiatives. Recommendation for Researchers: As a new phenomenon with uncharted potential, e-Learning 3.0 should be placed at the centre of educational research. Impact on Society: The understanding of what drives the success of e-Learning 3.0 is fundamental for its implementation and for the progress of online education in this new stage of its evolution. Future Research: Future research ventures can include the design of quantitative and self-administered data collection instruments that can provide further insight into the elements of the framework.

Reversal of hypercholesterolemia in apolipoprotein E2 and apolipoprotein E3-Leiden transgenic mice by adenovirus-mediated gene transfer of the VLDL receptor

NARCIS (Netherlands)

Dijk, K.W. van; Vlijmen, B.J.M. van; Zee, A. van der; Hof, B. van 't; Boom, H. van der; Kobayashi, K.; Chan, L.; Havekes, L.M.; Hofker, M.H.

1998-01-01

We have investigated the interaction of apolipoprotein E2(Arg158- Cys) (apoE2) and apolipoprotein E3Leiden (apoE3-Leiden) with the very low density lipoprotein (VLDL) receptor in vivo and in vitro to define the possible role of this receptor in lipoprotein metabolism and atherosclerosis. The in vivo

The interactions in K{sub μ3} and K{sub e3} decay

Energy Technology Data Exchange (ETDEWEB)

McDowell, S. W. [Brazilian Centre of Research in Physics, Rio de Janeiro (Brazil)

1963-01-15

An attractive feature common to all present theories of weak interactions is the assumption that they result from the coupling (either direct or via intermediate bosons) of vector-axial vector currents. The V-A coupling of leptonic currents and baryonic currents conserving strangeness successfully explains the characteristic properties of μ-decay, π-decay and μ-decay. On the other hand, the leptonic decay modes of K-mesons provide the best source of information on the nature of the currents carrying strangeness. From K{sub μ2} one learns that there must exist an axial-vector current carrying strangeness; further, if the leptonic weak interaction is universal, one can explain the absence of the K{sub e2} mode by excluding a pseudo-scalar interaction. From K{sub μ3} and K{sub e3} decay one can obtain information on scalar, vector and tensor coupling of strangeness non-conserving currents with leptons. In recent experiments on K{sub e3} decay a good fitting of the pion spectrum was obtained with a constant vector form factor. Although regarding this result as strong evidence of pure vector coupling, one might still argue that other possibilities, such as a mixture of vector and tensor couplings with energy-dependent form factors, are not excluded. I shall discuss here, in the first place, some properties of the transition amplitudes which are independent of the structure of form factors. They provide a test of universality and a criterion for unambiguous determination of the nature of the weak interaction involved in these processes. Secondly, I shall discuss the structure of form factors, by means of dispersion relations and introducing explicitly the effect of the K*-resonance in the Kπ-interaction.

Modelaje Molecular de E-1-[4’-(3-(sustituido) acriloil) fenil]-3-tosilureas

OpenAIRE

León, Caritza; Colman, Trina; Domínguez, José

2012-01-01

Se presentan resultados del estudio de modelado molecular de una serie de compuestos del tipo E-1-[4’-(3-(sustituido) acriloil)fenil]-3- tosilureas, para las cuales se ha reportado actividad antimalárica como inhibidores del desarrollo in vitro de cepas de P. falciparum resistentes a la Cloroquina. El estudio revela que los compuestos más activos biológicamente muestran una clara superposición en el fragmento comprendido entre el carbono carbonilo del sistema α,β-insaturado y el átomo de azuf...

Search for narrow resonances in e+e- annihilation between 1.85 and 3.1 GeV with the KEDR detector

International Nuclear Information System (INIS)

Anashin, V.V.; Aulchenko, V.M.; Baldin, E.M.; Barladyan, A.K.; Barnyakov, A.Yu.; Barnyakov, M.Yu.; Baru, S.E.; Basok, I.Yu.; Beloborodova, O.L.; Blinov, A.E.; Blinov, V.E.; Bobrov, A.V.; Bobrovnikov, V.S.; Bogomyagkov, A.V.; Bondar, A.E.; Buzykaev, A.R.; Eidelman, S.I.; Grigoriev, D.N.; Glukhovchenko, Yu.M.; Gulevich, V.V.

2011-01-01

We report results of a search for narrow resonances in e + e - annihilation at center-of-mass energies between 1.85 and 3.1 GeV performed with the KEDR detector at the VEPP-4M e + e - collider. The upper limit on the leptonic width of a narrow resonance Γ ee R .Br(R→hadr)<120 eV has been obtained (at 90% C.L.).

Characterization of dissimilar welding: carbon steel E309L-E308L-ERNiCr3-ENiCrFe3 alloy 600; Caracterizacion de la soldadura disimilar: acero al carbono E309L-E308L-ERNiCr3-ENiCrFe3 aleacion 600

Energy Technology Data Exchange (ETDEWEB)

Mucino G, O.

2015-07-01

Most BWR type reactors have internal support components, which need to be attached to the inner surface by welding. Specifically, in these joints two materials interact, such as stainless steel and nickel base alloys. Nickel base alloys such as alloy 82 (ERNiCr3) and alloy 182 (ENiCrFe-3) are used for the joining of both dissimilar materials. For joints made with both nickel base alloys, the alloy 182 is prone to stress corrosion cracking (SCC); so it is essential to carry out studies related to this contribution material. In the nuclear industry any study related to this alloy is of importance because experience is gained in its behavior when is part of a system of an operation reactor. This work presents the characterization of the weld deposit of a stainless steel coating (with electrodes E309L and E308L) on a carbon steel plate type A36 and the joining with an Inconel 600 plate, simulating the joining of the internal coating of vessel and the heel of the support leg of the envelope of a BWR reactor. In this work, the mechanical and micro-structural characterization of the alloy deposit 182 was performed. (Author)

Data of evolutionary structure change: 1RAAA-3GD5E [Confc[Archive

Lifescience Database Archive (English)

Full Text Available 1RAAA-3GD5E 1RAA 3GD5 A E ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELL...LRDPFEAARGAHILYTDVWTHRLQLFEQYQINAAL------------LNC--AAAEAIVLHCLPAHRGEEITDEVMEGPRS...ID> 0 1RAA A 1RAAA 1RAA A 1RAAA1RAA A 1RAAA TEFSGNVPVLN

Three closely related (2E,2′E-3,3′-(1,4-phenylenebis[1-(methoxyphenylprop-2-en-1-ones]: supramolecular assemblies in one dimension mediated by hydrogen bonding and C—H...π interactions

Directory of Open Access Journals (Sweden)

Aijia Sim

2017-06-01

Full Text Available In the title compounds, (2E,2′E-3,3′-(1,4-phenylenebis[1-(2-methoxyphenylprop-2-en-1-one], C26H22O4 (I, (2E,2′E-3,3′-(1,4-phenylenebis[1-(3-methoxyphenylprop-2-en-1-one], C26H22O4 (II and (2E,2′E-3,3′-(1,4-phenylenebis[1-(3,4-dimethoxyphenylprop-2-en-1-one], C28H26O6 (III, the asymmetric unit consists of a half-molecule, completed by crystallographic inversion symmetry. The dihedral angles between the central and terminal benzene rings are 56.98 (8, 7.74 (7 and 7.73 (7° for (I, (II and (III, respectively. In the crystal of (I, molecules are linked by pairs of C—H...π interactions into chains running parallel to [101]. The packing for (II and (III, features inversion dimers linked by pairs of C—H...O hydrogen bonds, forming R22(16 and R22(14 ring motifs, respectively, as parts of [201] and [101] chains, respectively.

3-[(E-(4-Chlorobenzylideneamino]-1-phenylthiourea

Directory of Open Access Journals (Sweden)

Nur Nadia Dzulkifli

2011-04-01

Full Text Available In the title compound, C14H12ClN3S, the dihedral angle between the terminal benzene rings is 56.6 (2°; the benzene rings lie to the same side of the molecule. The major twist in the molecule occurs around the Car—N bond (ar is aromatic [C—N—C—C = 49.9 (5°]. The configuration about the N=C bond [1.271 (4 Å] is E. The amine H atoms lie on opposite sides of the molecule with one forming an intramolecular N—H...N(imine hydrogen bond and an S(5 ring. In the crystal, centrosymmetric dimers are formed via {...HNC=S}2 synthons.

Photon radiation in e+e-→hadrons at low energies with carlomat3.1

International Nuclear Information System (INIS)

Jegerlehner, Fred; Humboldt-Universitaet, Berlin; Kolodziej, Karol

2017-01-01

We present a sample of results for the cross sections of several processes of low energetic e + e - annihilation into final states containing pions accompanied by one or two photons, or a light lepton pair. The results, which have been obtained with a new version of a multipurpose Monte Carlo program carlomat, labelled 3.1, demonstrate new capabilities of the program which, among others, include a possibility of taking into account either the initial or final state radiation separately, or both at a time, and a possibility of inclusion of the electromagnetic charged pion form factor for processes with charged pion pairs. We also discuss some problems related to the U(1) electromagnetic gauge invariance.

Observation of γ rays > 1015 eV from Cygnus X-3

International Nuclear Information System (INIS)

Lloyd-Evans, J.; Coy, R.N.; Lambert, A.; Lapikens, J.; Patel, M.; Reid, R.J.O.; Watson, A.A.

1983-01-01

The X-ray binary system Cygnus X-3 is a source of particular interest. As well as emitting X-rays which are modulated with a 4.8-h period, it has been observed in 30-100 MeV γ rays by the SAS II satellite and several groups have detected γ rays in the TeV range showing the same period. Most recently, using the small extensive air shower array at Kiel, workers have found that the γ-ray spectrum of Cygnus X-3 extends above 2 x 10 15 eV, with an integral flux of (7.4 +- 3.2) x 10 -14 cm -2 s -1 . This paper confirms the Kiel observations and presents evidence that the γ-ray spectrum of Cygnus X-3 steepens above 10 16 eV. (author)

Pathfinding the Flight Advanced Stirling Convertor Design with the ASC-E3

Science.gov (United States)

Wong, Wayne A.; Wilson, Kyle; Smith, Eddie; Collins, Josh

2012-01-01

The Advanced Stirling Convertor (ASC) was initially developed by Sunpower, Inc. under contract to NASA Glenn Research Center (GRC) as a technology development project. The ASC technology fulfills NASA's need for high efficiency power convertors for future Radioisotope Power Systems (RPS). Early successful technology demonstrations between 2003 to 2005 eventually led to the expansion of the project including the decision in 2006 to use the ASC technology on the Advanced Stirling Radioisotope Generator (ASRG). Sunpower has delivered 22 ASC convertors of progressively mature designs to date to GRC. Currently, Sunpower with support from GRC, Lockheed Martin Space System Company (LMSSC), and the Department of Energy (DOE) is developing the flight ASC-F in parallel with the ASC-E3 pathfinders. Sunpower will deliver four pairs of ASC-E3 convertors to GRC which will be used for extended operation reliability assessment, independent validation and verification testing, system interaction tests, and to support LMSSC controller verification. The ASC-E3 and -F convertors are being built to the same design and processing documentation and the same product specification. The initial two pairs of ASC-E3 are built before the flight units and will validate design and processing changes prior to implementation on the ASC-F flight convertors. This paper provides a summary on development of the ASC technology and the status of the ASC-E3 build and how they serve the vital pathfinder role ahead of the flight build for ASRG. The ASRG is part of two of the three candidate missions being considered for selection for the Discovery 12 mission.

Water promoted allylic nucleophilic substitution reactions of (E)-1,3 diphenylallyl acetate

KAUST Repository

Ghorpade, Seema Arun; Sawant, Dinesh N; Makki, Arwa; Sekar, N; Eppinger, Jö rg

2017-01-01

Transition metal free, water based, greener protocol for allylic alkylation, allylic amination, O-allylation of (E)-1,3-diphenylallyl acetate is described. The developed methodology is applicable for a wide range of nucleophiles furnishing excellent yields of corresponding products up to 87% under mild reaction conditions. A Distinct effect of water and base is explored for allylic nucleophilic substitution reactions of (E)-1,3-diphenylallyl acetate.

Water promoted allylic nucleophilic substitution reactions of (E)-1,3 diphenylallyl acetate

KAUST Repository

Ghorpade, Seema Arun

2017-11-30

Transition metal free, water based, greener protocol for allylic alkylation, allylic amination, O-allylation of (E)-1,3-diphenylallyl acetate is described. The developed methodology is applicable for a wide range of nucleophiles furnishing excellent yields of corresponding products up to 87% under mild reaction conditions. A Distinct effect of water and base is explored for allylic nucleophilic substitution reactions of (E)-1,3-diphenylallyl acetate.

Measurements of sigma (e+e-→μ+-μ-+) in the energy range 1.2-3.0 GeV

International Nuclear Information System (INIS)

Alles-Borelli, V.; Bernardini, M.; Bollini, D.; Giusti, P.; Massam, T.; Monari, L.; Palmonari, F.; Valenti, G.; Zichichi, A.

1975-01-01

The analysis of 1466 events of the type e + e - →μ +- μ -+ , in the time-like range from 1.44 to 9.00 GeV 2 , shows that the absolute value of the cross-section and its energy dependence follow QED expectations within (+- 3.2%) and (+- 1.2%), respectively. (Auth.)

Thermal decomposition of vinyl- and allylsilane platinum(II complexes and platinum(II catalysed synthesis of (E,(E-1,4-diphenyl-1,3-butadiene

Directory of Open Access Journals (Sweden)

Paul P. Mebe

2008-12-01

Full Text Available Thermal stabilities of Pt(II complexes: K[PtCl3(CH2=CHSiMe3], K[PtCl3(CH2=CHCH2SiMe3], K[(acacPtCl(CH2=CHSiMe3] and [PtCl(CH2=CHCH2SiMe3]2, were examined. All complexes were found to be stable at room temperature but they decomposed without melting above about 90 oC. The allylsilane complex decomposed above about 125 oE,(E-1,4-diphenyl-1,3-butadiene was stereoselectively synthesised in good yield from (E-β-styrylsilane in the presence of Zeise’s salt.

Efeitos do Ácido L-Glutâmico e da Vitamina D3 no Desempenho e nas Anomalias Ósseas de Pintos de Corte

Directory of Open Access Journals (Sweden)

Silva Fernanda Alvares da

2001-01-01

Full Text Available Um experimento foi conduzido para estudar os efeitos de níveis de ácido L-Glutâmico (L-Glu e vitamina D3 (VD da dieta em pintos de corte de um dia, machos, Hubbard, recebendo dieta básica purificada, contendo todos os L-aminoácidos essenciais, minerais e vitaminas (exceto vitamina D3, suplementada com 5, 10 e 15% de L-Glu, combinados com 0, 5.000, 10.000 e 15.000 UI de vitamina D3. O experimento foi realizado utilizando-se esquema fatorial, em delineamento inteiramente casualizado 3 x 4, com quatro repetições de sete aves cada. O ganho de peso aumentou até o nível máximo estimado, de 8,56% de L-Glu e 15.000 UI de vitamina D3. A melhor taxa de conversão alimentar foi verificada com nível estimado de 8,40% de L-Glu. O maior consumo de ração estimado foi obtido com 8,48% de L-Glu e 15.000 UI de vitamina D3. Houve redução na incidência de problemas de pernas com 10% de L-Glu e 15.000 UI de vitamina D3. L-Glu estimado em 8,56% e 15.000 UI de vitamina D3 permitiu um melhor desempenho das aves, confirmando que esse aminoácido é boa fonte de nitrogênio não-específico para maximizar o desempenho e reduzir a incidência de problemas de pernas.

Photochemical Aryl Radical Cyclizations to Give (E-3-Ylideneoxindoles

Directory of Open Access Journals (Sweden)

Michael Gurry

2014-09-01

Full Text Available (E-3-Ylideneoxindoles are prepared in methanol in reasonable to good yields, as adducts of photochemical 5-exo-trig of aryl radicals, in contrast to previously reported analogous radical cyclizations initiated by tris(trimethylsilylsilane and azo-initiators that gave reduced oxindole adducts.

Osteoblast-like MC3T3-E1 Cells Prefer Glycolysis for ATP Production but Adipocyte-like 3T3-L1 Cells Prefer Oxidative Phosphorylation.

Science.gov (United States)

Guntur, Anyonya R; Gerencser, Akos A; Le, Phuong T; DeMambro, Victoria E; Bornstein, Sheila A; Mookerjee, Shona A; Maridas, David E; Clemmons, David E; Brand, Martin D; Rosen, Clifford J

2018-06-01

Mesenchymal stromal cells (MSCs) are early progenitors that can differentiate into osteoblasts, chondrocytes, and adipocytes. We hypothesized that osteoblasts and adipocytes utilize distinct bioenergetic pathways during MSC differentiation. To test this hypothesis, we compared the bioenergetic profiles of preosteoblast MC3T3-E1 cells and calvarial osteoblasts with preadipocyte 3T3L1 cells, before and after differentiation. Differentiated MC3T3-E1 osteoblasts met adenosine triphosphate (ATP) demand mainly by glycolysis with minimal reserve glycolytic capacity, whereas nondifferentiated cells generated ATP through oxidative phosphorylation. A marked Crabtree effect (acute suppression of respiration by addition of glucose, observed in both MC3T3-E1 and calvarial osteoblasts) and smaller mitochondrial membrane potential in the differentiated osteoblasts, particularly those incubated at high glucose concentrations, indicated a suppression of oxidative phosphorylation compared with nondifferentiated osteoblasts. In contrast, both nondifferentiated and differentiated 3T3-L1 adipocytes met ATP demand primarily by oxidative phosphorylation despite a large unused reserve glycolytic capacity. In sum, we show that nondifferentiated precursor cells prefer to use oxidative phosphorylation to generate ATP; when they differentiate to osteoblasts, they gain a strong preference for glycolytic ATP generation, but when they differentiate to adipocytes, they retain the strong preference for oxidative phosphorylation. Unique metabolic programming in mesenchymal progenitor cells may influence cell fate and ultimately determine the degree of bone formation and/or the development of marrow adiposity. © 2018 American Society for Bone and Mineral Research. © 2018 American Society for Bone and Mineral Research.

Data of evolutionary structure change: 1RAAA-3E2PM [Confc[Archive

Lifescience Database Archive (English)

Full Text Available 1RAAA-3E2PM 1RAA 3E2P A M ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQ----... 1RAA A 1RAAA KANPQ----PELLK 1 1RAA A 1RAAA... 345 ILE CA 291 1RAA A 1RAAA...dbChain> 1RAAA TEFSGNVPVLN HHHH

Biochemical function of typical and variant Arabidopsis thaliana U-box E3 ubiquitin-protein ligases.

Science.gov (United States)

Wiborg, Jakob; O'Shea, Charlotte; Skriver, Karen

2008-08-01

The variance of the U-box domain in 64 Arabidopsis thaliana (thale cress) E3s (ubiquitin-protein ligases) was used to examine the interactions between E3s and E2s (ubiquitin-conjugating enzymes). E2s and E3s are components of the ubiquitin protein degradation pathway. Seven U-box proteins were analysed for their ability to ubiquitinate proteins in vitro in co-operation with different E2s. All U-box domains exhibited ubiquitination activity and interacted productively with UBC4/5-type E2s. Three and four of the U-box domains mediated ubiquitin addition in the presence of UBC13 and UBC7 E2s respectively, but no productive interaction was observed with the UBC15 E2 tested. The activity of AtPUB54 [Arabidopsis thaliana (thale cress) plant U-box 54 protein] was dependent on Trp(266) in the E2-binding cleft, and the E2 selectivity was changed by substitution of this position. The function of the distant U-box protein, AtPUB49, representing a large family of eukaryotic proteins containing a U-box linked to a cyclophilin-like peptidyl-prolyl cis-trans isomerase domain, was characterized biochemically. AtPUB49 functioned both as a prolyl isomerase and a chaperone by catalysing cis-trans isomerization of peptidyl-prolyl bonds and dissolving protein aggregates. In conclusion, both typical and atypical Arabidopsis U-box proteins were active E3s. The overlap in the E3/E2 selectivity suggests that in vivo specificity is not determined only by the E3-E2 interactions, but also by other parameters, e.g. co-existence or interactions with additional domains. The biochemical functions of AtPUB49 suggest that the protein can be involved in folding or degradation of protein substrates. Similar functions can also be retained within a protein complex with separate chaperone and U-box proteins.

Plane-wave impulse approximation extraction of the neutron magnetic form factor from Quasi-Elastic 3(rvec H)e((rvec e),e(prime)) at Q2 = 0.3 to 0.6 (GeV/c)2

International Nuclear Information System (INIS)

Xu, W.; Anderson, B.; Auberbach, L.; Averett, T.; Bertozzi, W.; Black, T.; Calarco, J.; Cardman, L.; Cates, G.D.; Chai, Z.W.; Chen, J.P.; Choi, S.; Chudakov, E.; Churchwell, S.; Corrado, G.S.; Crawford, C.; Dale, D.; Deur, A.; Djawotho, P.; Donnelly, T.W.; Dutta, D.; Finn, J.M.; Gao, H.; Gilman, R.; Glamazdin, A.V.; Glashausser, C.; Gloeckle, Walter; Golak, J.; Gomez, J.; Gorbenko, V.G.; Hansen, J.O.; Hersman, F.W.; Higinbotham, D.W.; Holmes, R.; Howell, C.R.; Hughes, E.; Humensky, B.; Incerti, S.; Jager, C.W. de; Jensen, J.S.; Jiang, X.; Jones, C.E.; Jones, M.; Kahl, R.; Kamada, H.; Kievsky, A.; Kominis, I.; Korsch, W.; Kramer, K.; Kumbartzki, G.; Kuss, M.; Lakuriqi, E.; Liang, M.; Liyanage, N.; LeRose, J.; Malov, S.; Margaziotis, D.J.; Martin, J.W.; McCormick, K.; McKeown, R. D.; McIlhany, K.; Meziani, Z.E.; Michaels, R.; Miller, G.W.; Mitchell, J.; Nanda, S.; Pace, E.; Pavlin, T.; Petratos, G.G.; Pomatsalyuk, R.I.; Pripstein, D.; Prout, D.; Ransome, R.D.; Roblin, Y.; Rvachev, M.; Saha, A.; Salme, G.; Schnee, M.; Shin, T.; Slifer, K.; Souder, P.A.; Strauch, S.; Suleiman, R.; Sutter, M.; Tipton, B.; Todor, L.; Viviani, M.; Vlahovic, B.; Watson, J.; Williamson, C.F.; Witala, H.; Wojtsekhowski, B.; Xiong, F.; Yeh, J.; Zolnierczuk, P.

2003-01-01

A high precision measurement of the transverse spin-dependent asymmetry A T in 3 (rvec H)e((rvec e),e(prime)) quasielastic scattering was performed in Hall A at Jefferson Lab at values of the squared four-momentum transfer, Q 2 , between 0.1 and 0.6 (GeV/c) 2 . A T is sensitive to the neutron magnetic form factor, G M n . Values of G M n at Q 2 = 0.1 and 0.2 (GeV/c) 2 , extracted using Faddeev calculations, were reported previously. Here, we report the extraction of G M n for the remaining Q 2 -values in the range from 0.3 to 0.6 (GeV/c) 2 using a Plane-Wave Impulse Approximation calculation. The results are in good agreement with recent precision data from experiments using a deuterium target

Site environmental report for calendar year 2002. DOE operations at the Boeing Company, Rocketdyne Propulsion and Power

Energy Technology Data Exchange (ETDEWEB)

None

2003-09-30

This Annual Site Environmental Report (ASER) for 2002 describes the environmental conditions related to work performed for the Department of Energy (DOE) at Area IV of Boeing' s Santa Susana Field Laboratory (SSFL)). In the past, the Energy Technology Engineering Center (ETEC), a government-owned, company-operated test facility, was located in Area IV. The operations at ETEC included development, fabrication, and disassembly of nuclear reactors, reactor fuel, and other radioactive materials. Other activities at ETEC involved the operation of large-scale liquid metal facilities that were used for testing liquid metal fast breeder components. All nuclear work was terminated in 1988, and, subsequently, all radiological work has been directed toward decontamination and decommissioning (D&D) of the former nuclear facilities and their associated sites. Closure of the liquid metal test facilities began in 1996. Results of the radiological monitoring program for the calendar year 2002 continue to indicate that there are no significant releases of radioactive material from Area IV of SSFL. All potential exposure pathways are sampled and/or monitored, including air, soil, surface water, groundwater, direct radiation, transfer of property ( land, structures, waste), and recycling. All radioactive w astes are processed for disposal at DOE disposal sites and/or other licensed sites approved by DOE for radioactive waste disposal. No liquid radioactive wastes are released into the environment, and no structural debris from buildings w as transferred to municipal landfills or recycled in 2002.

E3 analysis for crude and vacuum distillation system

Energy Technology Data Exchange (ETDEWEB)

Mittal, V; Zhang, J; Yang, X; Xu, Q [Dan F. Smith Department of Chemical Engineering, Lamar University, Beaumont, Texas (United States)

2011-11-15

Crude oil blending is a very common practice in petroleum refineries, where the main focus is to minimize the total purchase cost of crude oils under specified blending oil properties. Crude oil blending actually has significant impacts on energy consumption from heating furnaces during crude oil processing. Conceivably, furnace energy consumption from burning fuels such as natural gas, fuel oil, or propane causes huge amounts of CO{sub 2} emissions. In this paper, a methodology framework for crude oil blending and processing with simultaneous consideration of energy, emission, and economic profit (E3) is developed. It includes four stages of work: steady-state modeling, heating energy consumption calculation, emission model development, and economic evaluation. With Aspen HYSYS simulation, the developed methodology provides a quantitative support for refinery to identify an optimal E3 operating strategy. A case study is implemented to demonstrate the efficacy of this methodology. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

E2F3a gene expression has prognostic significance in childhood acute lymphoblastic leukemia.

Science.gov (United States)

Wang, Kai-Ling; Mei, Yan-Yan; Cui, Lei; Zhao, Xiao-Xi; Li, Wei-Jing; Gao, Chao; Liu, Shu-Guang; Jiao, Ying; Liu, Fei-Fei; Wu, Min-Yuan; Ding, Wei; Li, Zhi-Gang

2014-10-01

To study E2F3a expression and its clinical significance in children with acute lymphoblastic leukemia (ALL). We quantified E2F3a expression at diagnosis in 148 children with ALL by real-time PCR. In the test cohort (n = 48), receiver operating characteristic (ROC) curve was used to find the best cut-off point to divide the patients into E2F3a low- and high-expression groups. The prognostic significance of E2F3a expression was investigated in the test cohort and confirmed in the validation cohort (n = 100). The correlations of E2F3a expression with the clinical features and treatment outcome of these patients were analyzed. ROC curve analysis indicated that the best cut-off point of E2F3a expression was 0.3780. In the test cohort, leukemia-free survival (LFS) and event-free survival (EFS) of the low-expression group were lower than those of the high-expression group (log rank: P = 0.026 for both). This finding was verified in the validation cohort. LFS, EFS, and overall survival were also lower in the low-expression group than in the high-expression group (log rank, P = 0.015, 0.008, and 0.002 respectively). E2F3a low expression was correlated with the existence of BCR-ABL fusion. An algorithm composed of E2F3a expression and minimal residual disease (MRD) could predict relapse or induction failure more precisely than current risk stratification. These results were still significant in the ALL patients without BCR-ABL fusion. Low expression of E2F3a was associated with inferior prognosis in childhood ALL. An algorithm composed of E2F3a expression and MRD could predict relapse or induction failure more precisely than that of the current risk stratification. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Fast optical readout for Mu3e experiment

Energy Technology Data Exchange (ETDEWEB)

Huang, Qinhua [Institut fuer Kernphysik, Universitaet Mainz, Mainz (Germany); Collaboration: Mu3e-Collaboration

2016-07-01

Charged lepton flavour violation is highly suppressed in the Standard Model, which results in a prediction for the branching ratio of μ{sup +}→e{sup +}e{sup +}e{sup -} below O(10{sup -54}). The Mu3e experiment will search for this rare decay with a sensitivity of 10{sup -16}. An observation would be a clear sign for new physics. A high muon stopping rate of 2.10{sup 9} Hz is required so that sufficient statistics can be accumulated in about one year of data taking. The high event rate and the requirement of a full online track reconstruction demand a fast readout system which should provide a bandwidth above 1 Tbit/s. Reconfigurable devices, namely FPGAs, can easily parallelise the data processing, so it becomes possible to sort, merge, pack and route the data with low latency at high throughput. Optical fibres are the only option for the interconnection between different FPGA-based boards. The fibres also reduce the crosstalk and signal attenuation, especially over long distance links. As part of the readout system prototyping, firmware for synchronous merging of different data streams is being developed. In addition, the optical links have been tested and show a bit error rate below O(10{sup -16}) at 6.4 Gbit/s for a single fibre.

3D-e-Chem-VM: Structural Cheminformatics Research Infrastructure in a Freely Available Virtual Machine.

Science.gov (United States)

McGuire, Ross; Verhoeven, Stefan; Vass, Márton; Vriend, Gerrit; de Esch, Iwan J P; Lusher, Scott J; Leurs, Rob; Ridder, Lars; Kooistra, Albert J; Ritschel, Tina; de Graaf, Chris

2017-02-27

3D-e-Chem-VM is an open source, freely available Virtual Machine ( http://3d-e-chem.github.io/3D-e-Chem-VM/ ) that integrates cheminformatics and bioinformatics tools for the analysis of protein-ligand interaction data. 3D-e-Chem-VM consists of software libraries, and database and workflow tools that can analyze and combine small molecule and protein structural information in a graphical programming environment. New chemical and biological data analytics tools and workflows have been developed for the efficient exploitation of structural and pharmacological protein-ligand interaction data from proteomewide databases (e.g., ChEMBLdb and PDB), as well as customized information systems focused on, e.g., G protein-coupled receptors (GPCRdb) and protein kinases (KLIFS). The integrated structural cheminformatics research infrastructure compiled in the 3D-e-Chem-VM enables the design of new approaches in virtual ligand screening (Chemdb4VS), ligand-based metabolism prediction (SyGMa), and structure-based protein binding site comparison and bioisosteric replacement for ligand design (KRIPOdb).

In the absence of endogenous mouse apolipoprotein E, apolipoprotein E*2(Arg-158 → Cys) transgenic mice develop more severe hyperlipoproteinemia than apolipoprotein E*3-Leiden transgenic mice

NARCIS (Netherlands)

Vlijmen, B.J.M. van; Dijk, K.W. van; Hof, H.B. van 't; Gorp, P.J.J. van; Zee, A. van der; Boom, H. van der; Breuer, M.L.; Hofker, M.H.; Havekesf, L.M.

1996-01-01

Apolipoprotein E*2(Arg-155 → Cys) (APOE*2) transgenic mice were generated and compared to the previously generated apolipoprotein E*3- Leiden (APOE*3-Leiden) transgenic mice to study the variable expression of hyperlipoproteinemia associated with these two APOE variants. In the presence of the

Ácido Giberélico (GA3 e maturação de frutos das tangerinas 'Mexerica Montenegrina'e 'Poncã'

Directory of Open Access Journals (Sweden)

Marur Celso Jamil

1999-01-01

Full Text Available Na região norte do estado do Paraná, Brasil, as tangerinas `Ponkan' (Citrus reticulata e `Montenegrina' (Citrus deliciosa geralmente são colhidas em maio e julho, respectivamente. Aplicações de produtos à base de GA3 podem estender o período de produção, permitindo aos produtores programar a colheita e obter melhores preços. Neste trabalho foi estudado o efeito do GA3 sobre a maturação dos frutos de ambas as espécies, nos anos de 1996 e 1997. Os tratamentos constaram de concentrações compreendidas entre 20 e 60 mg L-1 de GA3 aplicadas ao início da mudança de cor dos frutos e quando a coloração alaranjada estava mais avançada. A cada 21 dias a maturação dos frutos foi visualmente avaliada e 6 frutos por parcela foram amostrados para análises do grau Brix e acidez. Com os resultados obtidos, pode-se concluir que a aplicação do produto parece ser mais eficiente quando efetuada no início de mudança de coloração do fruto, por mantê-los verdes por um período maior. Para ambas as espécies parece não ter havido diferenças entre as concentrações do produto, que estenderam o período de colheita por aproximadamente 3 semanas. Verificou-se, também, que o produto aplicado agiu somente na aparência da casca, sem alterar as características do suco.

μ→3e and μ→eγ decays in the model with neutrino and heavy neutral leptons mixing

International Nuclear Information System (INIS)

Ovchinnikova, A.A.

1978-01-01

Muon number nonconservation is discussed within the framework of the renormalizable model of Decker and Pestieau. To renormalize this model four heavy leptons are added. Then the gauge group SU(2)xU(1) is considered. Besides standard left doublets, the right ones in which mixing of neutrino and neutral heavy leptons takes place are introduced. In such a scheme the neutral current of charged leptons is a pure vector. Then the branching ratios of μ→3e and μ→eγ decays are evaluated. It has been shown that these quantities greatly depend upon neutrino masses. If neutrino has a mass about 0.65 MeV, the μ→eγ decay probability may well be close to its experimentally determined upper limit. It this model the μ→3e decay rate is a/π times less than the μ→eγ decay rate

GPM GROUND VALIDATION NOAA S-BAND PROFILER MINUTE DATA MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validation NOAA S-Band Profiler Minute Data MC3E dataset was gathered during the Midlatitude Continental Convective Clouds Experiment (MC3E) in...

E3: Economy - Energy - Environment; Supporting Manufacturing Leadership through Sustainability

Data.gov (United States)

U.S. Environmental Protection Agency — The E3 initiative is designed to help you thrive in a new business era focused on sustainability and, working together, to promote sustainable manufacturing and...

Comparison of global 3-D aviation emissions datasets

Directory of Open Access Journals (Sweden)

S. C. Olsen

2013-01-01

Full Text Available Aviation emissions are unique from other transportation emissions, e.g., from road transportation and shipping, in that they occur at higher altitudes as well as at the surface. Aviation emissions of carbon dioxide, soot, and water vapor have direct radiative impacts on the Earth's climate system while emissions of nitrogen oxides (NO_x, sulfur oxides, carbon monoxide (CO, and hydrocarbons (HC impact air quality and climate through their effects on ozone, methane, and clouds. The most accurate estimates of the impact of aviation on air quality and climate utilize three-dimensional chemistry-climate models and gridded four dimensional (space and time aviation emissions datasets. We compare five available aviation emissions datasets currently and historically used to evaluate the impact of aviation on climate and air quality: NASA-Boeing 1992, NASA-Boeing 1999, QUANTIFY 2000, Aero2k 2002, and AEDT 2006 and aviation fuel usage estimates from the International Energy Agency. Roughly 90% of all aviation emissions are in the Northern Hemisphere and nearly 60% of all fuelburn and NO_x emissions occur at cruise altitudes in the Northern Hemisphere. While these datasets were created by independent methods and are thus not strictly suitable for analyzing trends they suggest that commercial aviation fuelburn and NO_x emissions increased over the last two decades while HC emissions likely decreased and CO emissions did not change significantly. The bottom-up estimates compared here are consistently lower than International Energy Agency fuelburn statistics although the gap is significantly smaller in the more recent datasets. Overall the emissions distributions are quite similar for fuelburn and NO_x with regional peaks over the populated land masses of North America, Europe, and East Asia. For CO and HC there are relatively larger differences. There are however some distinct differences in the altitude distribution

HECT E3 Ubiquitin Ligase Itch Functions as a Novel Negative Regulator of Gli-Similar 3 (Glis3 Transcriptional Activity.

Directory of Open Access Journals (Sweden)

Gary T ZeRuth

Full Text Available The transcription factor Gli-similar 3 (Glis3 plays a critical role in the generation of pancreatic ß cells and the regulation insulin gene transcription and has been implicated in the development of several pathologies, including type 1 and 2 diabetes and polycystic kidney disease. However, little is known about the proteins and posttranslational modifications that regulate or mediate Glis3 transcriptional activity. In this study, we identify by mass-spectrometry and yeast 2-hybrid analyses several proteins that interact with the N-terminal region of Glis3. These include the WW-domain-containing HECT E3 ubiquitin ligases, Itch, Smurf2, and Nedd4. The interaction between Glis3 and the HECT E3 ubiquitin ligases was verified by co-immunoprecipitation assays and mutation analysis. All three proteins interact through their WW-domains with a PPxY motif located in the Glis3 N-terminus. However, only Itch significantly contributed to Glis3 polyubiquitination and reduced Glis3 stability by enhancing its proteasomal degradation. Itch-mediated degradation of Glis3 required the PPxY motif-dependent interaction between Glis3 and the WW-domains of Itch as well as the presence of the Glis3 zinc finger domains. Transcription analyses demonstrated that Itch dramatically inhibited Glis3-mediated transactivation and endogenous Ins2 expression by increasing Glis3 protein turnover. Taken together, our study identifies Itch as a critical negative regulator of Glis3-mediated transcriptional activity. This regulation provides a novel mechanism to modulate Glis3-driven gene expression and suggests that it may play a role in a number of physiological processes controlled by Glis3, such as insulin transcription, as well as in Glis3-associated diseases.

GPM GROUND VALIDATION KICT NEXRAD MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validaiton KICT NEXRAD MC3E dataset was collected from April 22, 2011 to June 6, 2011 for the Midlatitude Continental Convective Clouds Experiment...

E3 Testing of Directed Energy Systems: A Challenging Future

National Research Council Canada - National Science Library

Brown, Lyndell R

2009-01-01

.... Compatibility testing and susceptibility to electromagnetic radiation is required. Standards, such as MIL-STD-464 and MIL-STD-237D, are being revised to include HPM levels and frequencies for E3 tests...

GPM GROUND VALIDATION CHILL RADAR MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The CHILL radar data for the Midlatitude Continental Convective Clouds Experiment (MC3E) held in Oklahoma were collected while the NASA ER-2 aircraft conducted a...

Some Preliminary Scientific Results of Chang'E-3 Mission

Science.gov (United States)

Zou, Y.; Li, W.; Zheng, Y.; Li, H.

2015-12-01

Chang'E-3 mission is the main task of Phase two of China Lunar Exploration Program (CLEP), and also is Chinese first probe of landing, working and roving on the moon. Chang'E-3 craft composed of a lander and a rover, and each of them carry four scientific payloads respectively. The landing site of Chang'E-3 was located at 44.12 degrees north latitude and 19.51 degrees west longitude, where is in the northern part of Imbrium Which the distance in its west direction from the landing site of former Soviet probe Luna-17 is about 400 km, and about 780km far from the landing site of Appolo-17 in its southeast direction. Unfortunately, after a series of scientific tests and exploration on the surface of the moon, the motor controller communication of the rover emerged a breakdown on January 16, 2014, which leaded the four payloads onboard the rover can't obtain data anymore. However, we have received some interesting scientific data which have been studied by Chinese scientists. During the landing process of Chang'E-3, the Landing camera got total 4673 images with the Resolution in millimeters to meters, and the lander and rover took pictures for each other at different point with Topography camera and Panoramic camera. We can find characteristic changes in celestial brightness with time by analyzing image data from Lunar-based Ultraviolet Telescope (LUT) and an unprecedented constraint on water content in the sunlit lunar exosphere seen by LUT). The figure observed by EUV camera (EUVC) shows that there is a transient weak area of the Earth's plasma sphere; This event took place about three hours. The scientists think that it might be related to the change of the particle density of mid-latitude ionosphere. The preliminary spectral and mineralogical results from the landing site are derived according to the data of Visible and Near-infrared Imaging Spectrometer (VNIS). Seven major elements including Mg, Al, Si, K, Ca, Ti and Fe have been identified by the Active Particle

Cuscuta chinensis seeds water extraction protecting murine osteoblastic MC3T3-E1 cells against tertiary butyl hydroperoxide induced injury.

Science.gov (United States)

Gao, Jian-mei; Li, Ran; Zhang, Lei; Jia, Li-long; Ying, Xi-xiang; Dou, De-qiang; Li, Jian-chun; Li, Hai-bo

2013-07-09

Cuscuta chinensis (C. chinensis) is a well-known traditional Chinese herb that has been used to treat heart disease, diabetes, liver injury, cancer, and aging. Murine osteoblastic MC3T3-E1 cells were treated with various concentrations of C. chinensis water extraction at different time intervals. The antioxidant effect of C. chinensis on MC3T3-E1 cells was evaluated using MTT and TUNEL assays. The effect of C. chinensis on cell cycle was analyzed by flow cytometry with propidium iodide. Lipid peroxidation was measured by the HPLC method. The cellular redox status was determined from the reduced glutathione to oxidized glutathione ratio (GSH/GSSG) and the enzymes involved in glutathione metabolism, including glutathione reductase (GR), Glutathione S-transferase (GST), and Glucose-6-phosphate dehydrogenase (G6PD). The changes in relative mitochondrial transmembrane potential (ΔΨm) in the MC3T3-E1 cells were analyzed with rhodamine 123 staining. Western blot analysis was used to evaluate the levels of cytochrome c (cyto c), Bax, Bcl-2, caspase 3, Sirt3, and IDH2 expressions. The C. chinensis water extraction protects tertiary butyl hydroperoxide (TBHP)-treated MC3T3-E1 cells from death in a dose-dependent manner. C. chinensis treatment significantly inhibited the reactive oxygen species (ROS) generation, malondialdehyde (MDA) production, and increased the activity of superoxide dismutase (SOD), GR, GST, and G6PD. The release of cyto c from mitochondria was reduced by C. chinensis, which increased the expression of antiapoptotic IDH2, Sirt3, and Bcl-2 and decreased the expression of Bax, cyto c, and caspase 3. C. chinensis modulated the oxidative stress-induced apoptosis in MC3T3-E1 cells, probably due to its antioxidant activity and functioning via mitochondria-dependent pathways. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Phosphatidyl inositol-3 kinase (PIK3CA) E545K mutation confers cisplatin resistance and a migratory phenotype in cervical cancer cells

Science.gov (United States)

Arjumand, Wani; Merry, Cole D.; Wang, Chen; Saba, Elias; McIntyre, John B.; Fang, Shujuan; Kornaga, Elizabeth; Ghatage, Prafull; Doll, Corinne M.; Lees, Susan P.

2016-01-01

The phosphatidylinositol-3 kinase (PI3K)/Akt/mTOR signaling pathway is activated in many human cancers. Previously, we reported that patients with early stage cervical cancer whose tumours harbour PIK3CA exon 9 or 20 mutations have worse overall survival in response to treatment with radiation and cisplatin than patients with wild-type PIK3CA. The purpose of this study was to determine whether PIK3CA-E545K mutation renders cervical cancer cells more resistant to cisplatin and/or radiation, and whether PI3K inhibition reverses the phenotype. We found that CaSki cells that are heterozygous for the PIK3CA-E545K mutation are more resistant to cisplatin or cisplatin plus radiation than either HeLa or SiHa cells that express only wild-type PIK3CA. Similarly, HeLa cells engineered to stably express PIK3CA-E545K were more resistant to cisplatin or cisplatin plus radiation than cells expressing only wild-type PIK3CA or with PIK3CA depleted. Cells expressing the PIK3CA-E545K mutation also had constitutive PI3K pathway activation and increased cellular migration and each of these phenotypes was reversed by treatment with the PI3K inhibitor GDC-0941/Pictilisib. Our results suggests that cervical cancer patients whose tumours are positive for the PIK3CA-E545K mutation may benefit from PI3K inhibitor therapy in concert with standard cisplatin and radiation therapy. PMID:27489350

X-1E on Lakebed

Science.gov (United States)

1955-01-01

The Bell Aircraft Corporation X-1E in 1955 on the Rogers Dry Lakebed near the NACA High-Speed Flight Station, Edwards, California. The X-1E was notable for being shorter, with a thinner wing than the X-1A, -B, and -D. Aerodynamic heating caused the ailerons, rudder, and elevators to remain unpainted throughout the X-1E's flight test program. When the ventral fins were added, they were left unpainted too. On August 31, 1956, the aircraft reached a top speed of 1,480 miles per hour (Mach 2.24). There were four versions of the Bell X-1 rocket-powered research aircraft that flew at the NACA High-Speed Flight Research Station, Edwards, California. The bullet-shaped X-1 aircraft were built by Bell Aircraft Corporation, Buffalo, N.Y. for the U.S. Army Air Forces (after 1947, U.S. Air Force) and the National Advisory Committee for Aeronautics (NACA). The X-1 Program was originally designated the XS-1 for EXperimental Supersonic. The X-1's mission was to investigate the transonic speed range (speeds from just below to just above the speed of sound) and, if possible, to break the 'sound barrier.' Three different X-1s were built and designated: X-1-1, X-1-2 (later modified to become the X-1E), and X-1-3. The basic X-1 aircraft were flown by a large number of different pilots from 1946 to 1951. The X-1 Program not only proved that humans could go beyond the speed of sound, it reinforced the understanding that technological barriers could be overcome. The X-1s pioneered many structural and aerodynamic advances including extremely thin, yet extremely strong wing sections; supersonic fuselage configurations; control system requirements; powerplant compatibility; and cockpit environments. The X-1 aircraft were the first transonic-capable aircraft to use an all-moving stabilizer. The flights of the X-1s opened up a new era in aviation. The first X-1 was air-launched unpowered from a Boeing B-29 Superfortress on January 25, 1946. Powered flights began in December 1946. On October 14

17 CFR 270.22e-3 - Exemption for liquidation of money market funds.

Science.gov (United States)

2010-04-01

... money market funds. 270.22e-3 Section 270.22e-3 Commodity and Securities Exchanges SECURITIES AND... for liquidation of money market funds. (a) Exemption. A registered open-end management investment company or series thereof (“fund”) that is regulated as a money market fund under § 270.2a-7 is exempt...

GPM GROUND VALIDATION PAWNEE RADAR MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The Pawnee radar data for the Midlatitude Continental Convective Clouds Experiment (MC3E) held in Oklahoma were collected on May 24, 2011 to support the CHILL radar...

Effects of Δ-isobar degrees of freedom on the reactions 3He(n,γ)4He and 3He(p,e+νe)4He at low-energy

International Nuclear Information System (INIS)

Schiavilla, R.

1991-01-01

The cross sections of the radiative 3 He(n,γ) 4 He and weak 3 He(p,e + ν e ) 4 He capture reactions at thermal neutron and keV proton energies have been calculated with the Variational Monte Carlo method. The ground state and low-energy continuum wave functions have been determined variationally from a realistic Hamiltonian, and include both nucleon and Δ-isobar degrees of freedom. The electroweak transition operator contains one- and two-body components in the N + Δ Hilbert space

Ghosts of Milky Way's past: the globular cluster ESO 37-1 (E 3)

Science.gov (United States)

de la Fuente Marcos, R.; de la Fuente Marcos, C.; Moni Bidin, C.; Ortolani, S.; Carraro, G.

2015-09-01

Context. In the Milky Way, most globular clusters are highly conspicuous objects that were found centuries ago. However, a few dozen of them are faint, sparsely populated systems that were identified largely during the second half of the past century. One of the faintest is ESO 37-1 (E 3) and as such it remains poorly studied, with no spectroscopic observations published so far although it was discovered in 1976. Aims: We investigate the globular cluster E 3 in an attempt to better constrain its fundamental parameters. Spectroscopy of stars in the field of E 3 is shown here for the first time. Methods: Deep, precise VI CCD photometry of E 3 down to V ~ 26 mag is presented and analysed. Low-resolution, medium signal-to-noise ratio spectra of nine candidate members are studied to derive radial velocity and metallicity. Proper motions from the UCAC4 catalogue are used to explore the kinematics of the bright members of E 3. Results: Isochrone fitting indicates that E 3 is probably very old, with an age of about 13 Gyr; its distance from the Sun is nearly 10 kpc. It is also somewhat metal rich with [Fe/H] = -0.7. Regarding its kinematics, our tentative estimate for the proper motions is (μα cosδ,μδ) = (-7.0 ± 0.8, 3.5 ± 0.3) mas yr-1 (or a tangential velocity of 382 ± 79 km s-1) and for the radial velocity 45 ± 5 km s-1 in the solar rest frame. Conclusions: E 3 is one of the most intriguing globular clusters in the Galaxy. Having an old age and being metal rich is clearly a peculiar combination, only seen in a handful of objects like the far more conspicuous NGC 104 (47 Tucanae). In addition, its low luminosity and sparse population make it a unique template for the study of the final evolutionary phases in the life of a star cluster. Unfortunately, E 3 is among the most elusive and challenging known globular clusters because field contamination severely hampers spectroscopic studies. This research note is based on observations made with the ESO VLT at the

Metal-metal bonding and aromaticity in [M2(NHCHNH)3]2 (μ-E)2 (E = O, S; M = Nb, Mo, Tc, Ru, Rh).

Science.gov (United States)

Yan, Xiuli; Meng, Lingpeng; Sun, Zheng; Li, Xiaoyan

2016-02-01

The nature of M-M bonding and aromaticity of [M2(NHCHNH)3]2(μ-E)2 (E = O, S; M = Nb, Mo, Tc, Ru, Rh) was investigated using atoms in molecules (AIM) theory, electron localization function (ELF), natural bond orbital (NBO) and molecular orbital analysis. These analyses led to the following main conclusions: in [M2(NHCHNH)3]2(μ-E)2 (E = O, S; M = Nb, Mo, Tc, Ru, Rh), the Nb-Nb, Ru-Ru, and Rh-Rh bonds belong to "metallic" bonds, whereas Mo-Mo and Tc-Tc drifted toward the "dative" side; all these bonds are partially covalent in character. The Nb-Nb, Mo-Mo, and Tc-Tc bonds are stronger than Ru-Ru and Rh-Rh bonds. The M-M bonds in [M2(NHCHNH)3]2(μ-S)2 are stronger than those in [M2(NHCHNH)3]2(μ-O)2 for M = Nb, Mo, Tc, and Ru. The NICS(1)ZZ values show that all of the studied molecules, except [Ru2(NHCHNH)3]2(μ-O)2, are aromaticity molecules. O-bridged compounds have more aromaticity than S-bridged compounds. Graphical Abstract Left Molecular graph, and right electron localization function (ELF) isosurface of [M2(NHCHNH)3]2(μ-E)2(E = O, S; M = Nb, Mo, Tc, Ru, Rh).

Interacting mechanism of ID3 HLH domain towards E2A/E12 transcription factor – An Insight through molecular dynamics and docking approach

Directory of Open Access Journals (Sweden)

Nishith Saurav Topno

2016-03-01

Full Text Available Inhibitor of DNA binding protein 3 (ID3 has long been characterized as an oncogene that implicates its functional role through its Helix–Loop–Helix (HLH domain upon protein–protein interaction. An insight into the dimerization brought by this domain helps in identifying the key residues that favor the mechanism behind it. Molecular dynamics (MD simulations were performed for the HLH proteins ID3 and Transcription factor E2-alpha (E2A/E12 and their ensemble complex (ID3-E2A/E12 to gather information about the HLH domain region and its role in the interaction process. Further evaluation of the results by Principal Component Analysis (PCA and Free Energy Landscape (FEL helped in revealing residues of E2A/E12: Lys570, Ala595, Val598, and Ile599 and ID3: Glu53, Gln63, and Gln66 buried in their HLH motifs imparting key roles in dimerization process. Furthermore the T-pad analysis results helped in identifying the key fluctuations and conformational transitions using the intrinsic properties of the residues present in the domain region of the proteins thus specifying their crucial role towards molecular recognition. The study provides an insight into the interacting mechanism of the ID3-E2A/E12 complex and maps the structural transitions arising in the essential conformational space indicating the key structural changes within the helical regions of the motif. It thereby describes how the internal dynamics of the proteins might regulate their intrinsic structural features and its subsequent functionality.

(E-N′-[(E-3-(4-Hydroxy-3-methoxyphenylallylidene]isonicotinohydrazide

Directory of Open Access Journals (Sweden)

H. S. Naveenkumar

2010-02-01

Full Text Available In the title compound, C16H15N3O3, the dihedral angle between the pyridine and benzene rings is 7.66 (5°. The crystal packing is consolidated by intermolecular C—H...O and O—H...N interactions, which link the molecules into zigzag chains propagating along [010]. The chains are further linked into a three-dimensional network by N—H...O, C—H...N, C—H...O and C—H...π interactions.

PXR agonism decreases plasma HDL levels in ApoE*3-Leiden.CETP mice

NARCIS (Netherlands)

Haan, W. de; Vries-van der Weij, J. de; Mol, I.M.; Hoekstra, M.; Romijn, J.A.; Jukema, J.W.; Havekes, L.M.; Princen, H.M.G.; Rensen, P.C.N.

2009-01-01

Pregnane X receptor (PXR) agonism has been shown to affect multiple steps in both the synthesis and catabolism of HDL, but its integrated effect on HDL metabolism in vivo remains unclear. The aim of this study was to evaluate the net effect of PXR agonism on HDL metabolism in ApoE*3-Leiden (E3L) and

Measurement of Double-Polarization Asymmetries in the Quasielastic He3→(e → ,e'd ) Process

Science.gov (United States)

Mihovilovič, M.; Jin, G.; Long, E.; Zhang, Y.-W.; Allada, K.; Anderson, B.; Annand, J. R. M.; Averett, T.; Boeglin, W.; Bradshaw, P.; Camsonne, A.; Canan, M.; Cates, G. D.; Chen, C.; Chen, J. P.; Chudakov, E.; De Leo, R.; Deng, X.; Deltuva, A.; Deur, A.; Dutta, C.; El Fassi, L.; Flay, D.; Frullani, S.; Garibaldi, F.; Gao, H.; Gilad, S.; Gilman, R.; Glamazdin, O.; Golak, J.; Golge, S.; Gomez, J.; Hansen, O.; Higinbotham, D. W.; Holmstrom, T.; Huang, J.; Ibrahim, H.; de Jager, C. W.; Jensen, E.; Jiang, X.; Jones, M.; Kang, H.; Katich, J.; Khanal, H. P.; Kievsky, A.; King, P.; Korsch, W.; LeRose, J.; Lindgren, R.; Lu, H.-J.; Luo, W.; Marcucci, L. E.; Markowitz, P.; Meziane, M.; Michaels, R.; Moffit, B.; Monaghan, P.; Muangma, N.; Nanda, S.; Norum, B. E.; Pan, K.; Parno, D.; Piasetzky, E.; Posik, M.; Punjabi, V.; Puckett, A. J. R.; Qian, X.; Qiang, Y.; Qui, X.; Riordan, S.; Saha, A.; Sauer, P. U.; Sawatzky, B.; Schiavilla, R.; Schoenrock, B.; Shabestari, M.; Shahinyan, A.; Širca, S.; Skibiński, R.; John, J. St.; Subedi, R.; Sulkosky, V.; Tobias, W. A.; Tireman, W.; Urciuoli, G. M.; Viviani, M.; Wang, D.; Wang, K.; Wang, Y.; Watson, J.; Wojtsekhowski, B.; Witała, H.; Ye, Z.; Zhan, X.; Zhang, Y.; Zheng, X.; Zhao, B.; Zhu, L.; Jefferson Lab Hall A Collaboration

2014-12-01

We present a precise measurement of double-polarization asymmetries in the He3→(e → ,e'd ) reaction. This particular process is a uniquely sensitive probe of hadron dynamics in 3He and the structure of the underlying electromagnetic currents. The measurements have been performed in and around quasielastic kinematics at Q2=0.25 (GeV /c )2 for missing momenta up to 270 MeV /c . The asymmetries are in fair agreement with the state-of-the-art calculations in terms of their functional dependencies on pm and ω , but are systematically offset. Beyond the region of the quasielastic peak, the discrepancies become even more pronounced. Thus, our measurements have been able to reveal deficiencies in the most sophisticated calculations of the three-body nuclear system, and indicate that further refinement in the treatment of their two-and/or three-body dynamics is required.

Characterization of dissimilar welding: carbon steel E309L-E308L-ERNiCr3-ENiCrFe3 alloy 600

International Nuclear Information System (INIS)

Mucino G, O.

2015-01-01

Most BWR type reactors have internal support components, which need to be attached to the inner surface by welding. Specifically, in these joints two materials interact, such as stainless steel and nickel base alloys. Nickel base alloys such as alloy 82 (ERNiCr3) and alloy 182 (ENiCrFe-3) are used for the joining of both dissimilar materials. For joints made with both nickel base alloys, the alloy 182 is prone to stress corrosion cracking (SCC); so it is essential to carry out studies related to this contribution material. In the nuclear industry any study related to this alloy is of importance because experience is gained in its behavior when is part of a system of an operation reactor. This work presents the characterization of the weld deposit of a stainless steel coating (with electrodes E309L and E308L) on a carbon steel plate type A36 and the joining with an Inconel 600 plate, simulating the joining of the internal coating of vessel and the heel of the support leg of the envelope of a BWR reactor. In this work, the mechanical and micro-structural characterization of the alloy deposit 182 was performed. (Author)

Behavior as a sentry of metal toxicity

Energy Technology Data Exchange (ETDEWEB)

Weiss, B.

1978-01-01

Many of the toxic properties of metals are expressed as behavioral aberrations. Some of these arise from direct actions on the central nervous system. Others arise from primary events elsewhere, but still influence behavior. Toxicity may be expressed either as objectively measurable phenomena, such as ataxia, or as subjective complaints, such as depression. In neither instance is clinical medicine equipped to provide assessments of subtle, early indices of toxicity. Reviewers of visual disturbances, paresthesia, and mental retardation exemplify the potential contribution of psychology to the toxicology of metals. Behavior and nervous system functions act as sensitive mirrors of metal toxicity. Sensitivity is the prime aim in environmental health assessments. Early detection of adverse effects, before they progress to irreversibility, underlies the strategy for optimal health protection. Some of the toxic actions of metals originate in direct nervous system dysfunction. Others may reflect disturbances of systems less directly linked to behavior than the central nervous system. But behavior, because it expresses the integrated functioning of the organism, can indicate flaws in states and processes outside the nervous system.

E2-EPF UCP Possesses E3 Ubiquitin Ligase Activity via Its Cysteine 118 Residue.

Science.gov (United States)

Lim, Jung Hwa; Shin, Hee Won; Chung, Kyung-Sook; Kim, Nam-Soon; Kim, Ju Hee; Jung, Hong-Ryul; Im, Dong-Soo; Jung, Cho-Rok

Here, we show that E2-EPF ubiquitin carrier protein (UCP) elongated E3-independent polyubiquitin chains on the lysine residues of von Hippel-Lindau protein (pVHL) and its own lysine residues both in vitro and in vivo. The initiation of the ubiquitin reaction depended on not only Lys11 linkage but also the Lys6, Lys48 and Lys63 residues of ubiquitin, which were involved in polyubiquitin chain formation on UCP itself. UCP self-association occurred through the UBC domain, which also contributed to the interaction with pVHL. The polyubiquitin chains appeared on the N-terminus of UCP in vivo, which indicated that the N-terminus of UCP contains target lysines for polyubiquitination. The Lys76 residue of UCP was the most critical site for auto-ubiquitination, whereas the polyubiquitin chain formation on pVHL occurred on all three of its lysines (Lys159, Lys171 and Lys196). A UCP mutant in which Cys118 was changed to alanine (UCPC118A) did not form a polyubiquitin chain but did strongly accumulate mono- and di-ubiquitin via auto-ubiquitination. Polyubiquitin chain formation required the coordination of Cys95 and Cys118 between two interacting molecules. The mechanism of the polyubiquitin chain reaction of UCP may involve the transfer of ubiquitin from Cys95 to Cys118 by trans-thiolation, with polyubiquitin chains forming at Cys118 by reversible thioester bonding. The polyubiquitin chains are then moved to the lysine residues of the substrate by irreversible isopeptide bonding. During the elongation of the ubiquitin chain, an active Cys118 residue is required in both parts of UCP, namely, the catalytic enzyme and the substrate. In conclusion, UCP possesses not only E2 ubiquitin conjugating enzyme activity but also E3 ubiquitin ligase activity, and Cys118 is critical for polyubiquitin chain formation.

E2-EPF UCP Possesses E3 Ubiquitin Ligase Activity via Its Cysteine 118 Residue.

Directory of Open Access Journals (Sweden)

Jung Hwa Lim

Full Text Available Here, we show that E2-EPF ubiquitin carrier protein (UCP elongated E3-independent polyubiquitin chains on the lysine residues of von Hippel-Lindau protein (pVHL and its own lysine residues both in vitro and in vivo. The initiation of the ubiquitin reaction depended on not only Lys11 linkage but also the Lys6, Lys48 and Lys63 residues of ubiquitin, which were involved in polyubiquitin chain formation on UCP itself. UCP self-association occurred through the UBC domain, which also contributed to the interaction with pVHL. The polyubiquitin chains appeared on the N-terminus of UCP in vivo, which indicated that the N-terminus of UCP contains target lysines for polyubiquitination. The Lys76 residue of UCP was the most critical site for auto-ubiquitination, whereas the polyubiquitin chain formation on pVHL occurred on all three of its lysines (Lys159, Lys171 and Lys196. A UCP mutant in which Cys118 was changed to alanine (UCPC118A did not form a polyubiquitin chain but did strongly accumulate mono- and di-ubiquitin via auto-ubiquitination. Polyubiquitin chain formation required the coordination of Cys95 and Cys118 between two interacting molecules. The mechanism of the polyubiquitin chain reaction of UCP may involve the transfer of ubiquitin from Cys95 to Cys118 by trans-thiolation, with polyubiquitin chains forming at Cys118 by reversible thioester bonding. The polyubiquitin chains are then moved to the lysine residues of the substrate by irreversible isopeptide bonding. During the elongation of the ubiquitin chain, an active Cys118 residue is required in both parts of UCP, namely, the catalytic enzyme and the substrate. In conclusion, UCP possesses not only E2 ubiquitin conjugating enzyme activity but also E3 ubiquitin ligase activity, and Cys118 is critical for polyubiquitin chain formation.

A novel chimeric peptide binds MC3T3‑E1 cells to titanium and enhances their proliferation and differentiation.

Science.gov (United States)

Wang, Dan; Liao, Xiaofu; Qin, Xu; Shi, Wei; Zhou, Bin

2013-05-01

Previous studies have demonstrated that the modification of the titanium (Ti) surface of an implant with RGD (Arg‑Gly‑Asp) promotes the activity of osteoblasts. A novel Ti‑binding peptide, minTBP‑1, and a chimeric peptide, minTBP‑1‑PRGDN, have been synthesized to assist the fixing of RGD to Ti. In our previous study, minTBP‑1‑PRGDN demonstrated favorable affinity for Ti surfaces and facilitated the adhesion of MC3T3‑E1 cells. The aim of the present study was to evaluate the effect of this chimeric peptide on the proliferation and differentiation of MC3T3‑E1 cells. For this purpose, MC3T3‑E1 cells were cultured and differentiation was induced on Ti discs precoated with minTBP‑1‑PRGDN, minTBP‑1 or PRGDN. The MC3T3‑E1 cells on the minTBP‑1‑PRGDN‑precoated Ti disc were observed to exhibit the highest cell number after 24 h and alkaline phosphatase levels in all groups increased in a time‑dependent manner. In addition, marked expression of osteogenic marker genes [osteopontin (OPN) and osteocalcin (OC)] was detected on minTBP‑1‑PRGDN/Ti at day 14. Mineralized deposits on minTBP‑1‑PRGDN/Ti presented the maximal average area and the highest number of deposits was observed on PRGDN/Ti. The present study indicates that minTBP‑1‑PRGDN may enhance and accelerate the activities of MC3T3‑E1 cells on Ti, however, its role in vivo must be determined by further studies.

Structure and resonances of the e+–He(1s2s 3Se) system

International Nuclear Information System (INIS)

Ren Zhenzhong; Han Huili; Shi Tingyun; Mitroy, J

2012-01-01

We have studied the geometry and resonances of the e + He( 3 S e ) system in the framework of hyperspherical coordinates. A model potential proposed by us is used to describe the interaction between the out electron with the He + ionic core. The calculated binding energy and expectation distance of the system are in agreement with other calculations. In addition, two resonances below the e + –He(1s3s 3 S e ) threshold and one resonance below the Ps(n=2)–He + threshold are identified. (paper)

Simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2, and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts

International Nuclear Information System (INIS)

Ritzi, E.M.; Stylos, W.A.

1976-01-01

The relative stability of Prostaglandins (PGs) E1, E2 and F1α in cultures of BALB/c 3T3 and SV3T3 cells has been evaluated using 3 different approaches. First, total recovery of tritium in the ethyl acetate phase following incubation and extraction of PGF1α and PGE1 demonstrated greater stability for PGF1α (88.8 percent) than PGE1 (65.9 percent). Second, analysis of incubated, extracted, tritiated PGs by thin layer chromatography revealed decreases of up to 23 percent in the PGE zone following incubation of 3H-PGE1. With increasing time of incubation, decreases in the PGE zone were accompanied by increase in PGA-like compounds. 3H-PGF1α demonstrated greater stability, having greater than 90 percent recovery of the tritium in the PGF zone. A third approach to the assessment of PG stability in culture was the comparison of the production of individual PGs by radioimmunoassay (RIA). The data obtained by RIA indicated a lag in the increase of PGA and PGB, until an initial rise in PGE was noted, suggesting that PGA and PGB may be secondary products arising from PGE which exhibits only partial stability in culture. By employing two RIAs, one for total PGE and one for PGA and PGB, the composite determination PG [E + (A + B)] can be used to provide a more meaningful determination of PG production because of the instability of the PGs. On the other hand, individual determinations are helpful in assessing the stability of PGEs in cell cultures

QCD demonstration for the color string structures of e+e-→q bar qg and Υ→3g systems

International Nuclear Information System (INIS)

Tian, L.; Xie, Q.; Si, Z.

1994-01-01

The e + e - →q bar qg→3 jets and Υ→3g→hadrons processes are well suited to the study of the gluon hadronization mechanism. In the LUND model, these two processes are treated by applying the string fragmentation model to the assumed color string structure of q bar qg and 3g systems. In this paper, the color string structure of the q bar qg and ggg systems is given by directly analyzing their color wave functions in the context of perturbative QCD. In addition, the reasonableness and accuracy of the LUND string pictures are discussed

Micorrização e indução de quitinases e β-1,3-glucanases e resistência à fusariose em porta-enxerto de videira Mycorrhizal inoculation and induction of chitinases and β-1,3-glucanases and fusarium resistance in grapevine rootstock

Directory of Open Access Journals (Sweden)

Murilo Dalla Costa

2010-04-01

Full Text Available O objetivo deste trabalho foi avaliar os níveis de expressão de β-1,3-glucanases e quitinases nos porta-enxertos de videira SO4 e R110, respectivamente suscetível e resistente a Fusarium oxysporum f. sp. herbemontis, bem como avaliar o efeito do fungo micorrízico arbuscular Glomus intraradices no crescimento, na expressão dessas enzimas e na supressão do patógeno no porta-enxerto suscetível. Foram quantificadas as atividades enzimáticas de β-1,3-glucanases e quitinases nas raízes dos porta-enxertos. Mudas do porta-enxerto SO4 receberam inóculos de G. intraradices e F. oxysporum, e foram avaliadas quanto ao crescimento, atividade das duas enzimas e sintomas de doença. As atividades das enzimas nas raízes do porta-enxerto resistente aumentaram entre 0 e 5 dias após a inoculação do patógeno. A atividade de quitinases nas raízes do porta-enxerto suscetível aumentou com a inoculação do fungo micorrízico e do patógeno. A atividade de β-1,3-glucanases foi maior somente com a presença do fungo micorrízico e do patógeno. Videiras com inoculação de G. intraradices apresentaram diminuição nos sintomas de infecção por Fusarium spp., o que indica que o fungo micorrízico promove a indução de quitinases e β-1,3-glucanases especificamente na supressão ou inibição do patógeno.The objective of this work was to evaluate the expression levels of β-1,3-glucanases and chitinases in SO4 and 110 grapevine rootstocks, respectively susceptible and resistant to Fusarium oxysporum f. sp. herbemontis, as well as to evaluate the effect of the arbuscular mycorrhizal fungus Glomus intraradices on plant growth, on enzyme expression and on pathogen suppression in the susceptible rootstock. The enzyme activities of β-1,3-glucanases and chitinases in the rootstocks roots were evaluated. Plant growth, enzyme activity, and disease symptoms were evaluated in SO4 plantlets inoculated with G. intraradices and F. oxysporum. Enzyme activities

Biochemical function of typical and variant Arabidopsis thaliana U-box E3 ubiquitin-protein ligases

DEFF Research Database (Denmark)

Wiborg, Jakob; O'Shea, Charlotte; Skriver, Karen

2008-01-01

of the distant U-box protein, AtPUB49, representing a large family of eukaryotic proteins containing a U-box linked to a cyclophilin-like peptidyl-prolyl cis-trans isomerase domain, was characterized biochemically. AtPUB49 functioned both as a prolyl isomerase and a chaperone by catalysing cis......The variance of the U-box domain in 64 Arabidopsis thaliana (thale cress) E3s (ubiquitin-protein ligases) was used to examine the interactions between E3s and E2s (ubiquitin-conjugating enzymes). E2s and E3s are components of the ubiquitin protein degradation pathway. Seven U-box proteins were...... analysed for their ability to ubiquitinate proteins in vitro in co-operation with different E2s. All U-box domains exhibited ubiquitination activity and interacted productively with UBC4/5-type E2s. Three and four of the U-box domains mediated ubiquitin addition in the presence of UBC13 and UBC7 E2s...

A study of the profile of the E3 electron trap in GaAs

Energy Technology Data Exchange (ETDEWEB)

Kourkoutas, C.D. (TEI Athens (Greece). Dept. of Physics Chemistry and Material Technology); Kovacs, B.; Szentpali, B.; Somogyi, K. (Research Inst. for Technical Physics, Budapest (Hungary)); Euthymiou, P.C. (Athens Univ. (Greece)); Giakoumakis, G.E. (Ioannina Univ. (Greece). Dept. of Physics)

1994-01-01

Electron irradiation at room temperature introduces in GaAs a donor type electronic state Tx at 0.18 eV, which is associated with the E3 electron trap. The presence of Tx is observed at depths d > 1.5 [mu]m, which correspond to the limits of the depletion region under the highest applied reverse bias voltage, while the E3 trap concentration drops off into the same region. (author).

A study of the profile of the E3 electron trap in GaAs

International Nuclear Information System (INIS)

Kourkoutas, C.D.; Euthymiou, P.C.; Giakoumakis, G.E.

1994-01-01

Electron irradiation at room temperature introduces in GaAs a donor type electronic state Tx at 0.18 eV, which is associated with the E3 electron trap. The presence of Tx is observed at depths d > 1.5 μm, which correspond to the limits of the depletion region under the highest applied reverse bias voltage, while the E3 trap concentration drops off into the same region. (author)

Tier 1 and Tier 3 eAdjudication Business Rule Validation

Science.gov (United States)

2018-04-01

correct rejections. • Research ways to safely approve more cases through eAdjudication. PERSEREC has established a business rule test environment that can... WORK UNIT NUMBER: 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Defense Personnel and Security Research Center Office of People Analytics 400...interagency working group of personnel security and suitability experts on business rule development for T3 and T3R. The results of rule development and

Case study of the interdisciplinary integration in an IST-E3 project

DEFF Research Database (Denmark)

Jørgensen, Ulrik

2002-01-01

Case study of a specific IST-E3 project funded by the EU commissions 5th framework program. The case study highlights the difficulties in integrating different disciplinary approaches and suggests that a more openended research strategy should be applied by the commission.......Case study of a specific IST-E3 project funded by the EU commissions 5th framework program. The case study highlights the difficulties in integrating different disciplinary approaches and suggests that a more openended research strategy should be applied by the commission....

STAT3 mutations correlated with hyper-IgE syndrome lead to ...

Indian Academy of Sciences (India)

Of all the causes identified for the disease hyper-immunoglobulinemia E syndrome (HIES), a homozygous mutation in tyrosine kinase2 (TYK2) and heterozygous mutations in STAT3 are implicated the defects in Jak/STAT signalling pathway in the pathogenesis of HIES. Mutations of STAT3 have been frequently clinically ...

GEM-E3: A computable general equilibrium model applied for Switzerland

International Nuclear Information System (INIS)

Bahn, O.; Frei, C.

2000-01-01

The objectives of the European Research Project GEM-E3-ELITE, funded by the European Commission and coordinated by the Centre for European Economic Research (Germany), were to further develop the general equilibrium model GEM-E3 (Capros et al., 1995, 1997) and to conduct policy analysis through case studies. GEM-E3 is an applied general equilibrium model that analyses the macro-economy and its interaction with the energy system and the environment through the balancing of energy supply and demand, atmospheric emissions and pollution control, together with the fulfillment of overall equilibrium conditions. PSI's research objectives within GEM-E3-ELITE were to implement and apply GEM-E3 for Switzerland. The first objective required in particular the development of a Swiss database for each of GEM-E3 modules (economic module and environmental module). For the second objective, strategies to reduce CO 2 emissions were evaluated for Switzerland. In order to develop the economic, PSI collaborated with the Laboratory of Applied Economics (LEA) of the University of Geneva and the Laboratory of Energy Systems (LASEN) of the Federal Institute of Technology in Lausanne (EPFL). The Swiss Federal Statistical Office (SFSO) and the Institute for Business Cycle Research (KOF) of the Swiss Federal Institute of Technology (ETH Zurich) contributed also data. The Swiss environmental database consists mainly of an Energy Balance Table and of an Emission Coefficients Table. Both were designed using national and international official statistics. The Emission Coefficients Table is furthermore based on know-how of the PSI GaBE Project. Using GEM-E3 Switzerland, two strategies to reduce the Swiss CO 2 emissions were evaluated: a carbon tax ('tax only' strategy), and the combination of a carbon tax with the buying of CO 2 emission permits ('permits and tax' strategy). In the first strategy, Switzerland would impose the necessary carbon tax to achieve the reduction target, and use the tax

Study of 3-jet events in e/sup +/e/sup -/ annihilation into hadrons at 34. 6 c. m. energy

Energy Technology Data Exchange (ETDEWEB)

Althoff, M; Braunschweig, W; Kirschfink, F J; Martyn, H U; Rosskamp, R; Schmitz, D; Siebke, H; Wallraff, W; Eisenmann, J; Fischer, H M

1985-10-01

Three-jet events produced by e/sup +/e/sup -/ annihilation into hadrons at 34.6 GeV c.m. energy were studied by comparing them with 2nd order QCD and two different models of fragmentation. The distribution of low energy particles in the 3-jet plane is found to be better described by the LUND color string model than by the independent jet model. The opposite is true for more energetic particles flowing between the 3 jets. The average transverse momenta in jets can be described with values of sigmasub(q) between 350 and 500 MeV/c for the gluon jet.

Influence of MC3T3-E1 preosteoblast culture on the corrosion of a T6-treated AZ91 alloy

Science.gov (United States)

Brooks, Emily K.; Tobias, Menachem E.; Yang, Shuying; Bone, Lawrence B.; Ehrensberger, Mark T.

2015-01-01

This study investigated the corrosion of artificially aged T6 heat-treated Mg-9%Al-1%Zn (AZ91) for biomedical applications. Corrosion tests and surface analysis were completed both with and without a monolayer of mouse preosteoblast MC3T3-E1 cells cultured on the sample. Electrochemical impedance spectroscopy (EIS) and inductively coupled plasma mass spectroscopy (ICPMS) were used to explore the corrosion processes after either 3 or 21 days of AZ91 incubation in cell culture medium (CCM). The EIS showed both the inner layer resistance (Rin) and outer layer resistance (Rout) were lower for samples without cells cultured on the surface at 3 days (Rin = 2.64 e4 Ω/cm2, Rout = 140 Ω/cm2) compared to 21 days (Rin = 3.60 e4 Ω/cm2, Rout = 287 Ω/cm2) due to precipitation of magnesium and calcium phosphates over time. Samples with preosteoblasts cultured on the surface had a slower initial corrosion (3 day, Rin = 1.88 e5 Ω/cm2, Rout = 1060 Ω/cm2) which was observed to increase over time (21 day, Rin = 2.99 e4 Ω/cm2, Rout = 287 Ω/cm2). Changes in the corrosion processes were thought to be related to changes in the coverage provided by the cell layer. Our results reveal that the presence of cells and biological processes are able to significantly influence the corrosion rate of AZ91. PMID:25715925

Synthesis and Antimycobacterial and Photosynthesis-Inhibiting Evaluation of 2-[(E-2-Substituted-ethenyl]-1,3-benzoxazoles

Directory of Open Access Journals (Sweden)

Ales Imramovsky

2014-01-01

Full Text Available A series of twelve 2-[(E-2-substituted-ethenyl]-1,3-benzoxazoles was designed. All the synthesized compounds were tested against three mycobacterial strains. The compounds were also evaluated for their ability to inhibit photosynthetic electron transport (PET in spinach (Spinacia oleracea L. chloroplasts. 2-[(E-2-(4-Methoxyphenylethenyl]-1,3-benzoxazole, 2-[(E-2-(2,3-dihydro-1-benzofuran-5-ylethenyl]-1,3-benzoxazole and 2-{(E-2-[4-(methylsulfanylphenyl]ethenyl}-1,3-benzoxazole showed the highest activity against M. tuberculosis, M. kansasii, and M. avium, and they demonstrated significantly higher activity against M. avium and M. kansasii than isoniazid. The PET-inhibiting activity of the most active ortho-substituted compound 2-[(E-2-(2-methoxyphenylethenyl]-1,3-benzoxazole was IC50 = 76.3 μmol/L, while the PET-inhibiting activity of para-substituted compounds was significantly lower. The site of inhibitory action of tested compounds is situated on the donor side of photosystem II. The structure-activity relationships are discussed.

cis- and trans-2,3,3a,4,5,9b-Hexahydro-1H-benz[e]indoles: synthesis and evaluation of dopamine D2, and D3 receptor binding affinity

DEFF Research Database (Denmark)

Song, Xiaodong; Crider, Michael A.; Cruse, Sharon F.

1999-01-01

cis- and trans-2,3,3a,4,5,9b-hexahydro-1H-benz [e]indoles were synthesized as conformationally rigid analogues of 3-phenylpyrrolidine and evaluated for dopamine (DA) D2S and D3 receptor binding affinity. The tricyclic benz[e]indole nucleus was constructed by a previously reported reductive...... configuration. These novel ligands may be useful tools for gaining additional information about the DA D3 receptor. Copyright Elsevier, Paris.dopamine / D2S receptor / D3 receptor / cis- and trans-2,3,3a,4,5,9b-hexahydro-1H-benz[e]indoles / receptor binding affinity....... receptors was shown by compounds substituted with N-n-propyl or N-allyl groups. The cis-(+-)-N-allyl derivative 21e demonstrated a D2S/D3 selectivity of 290. Resolution of cis-(+-)-5 and trans-(+-)- 21c into individual enantiomers showed that in both series the more active isomer had 3aR absolute...

Apolipoprotein E*3-Leiden transgenic mice mode for hypolipidaemic drugs

NARCIS (Netherlands)

Vlijmen, B.J.M. van; Pearce, N.J.; Bergö, M.; Staels, B.; Yates, J.W.; Gribble, A.D.; Bond, B.C.; Hofker, M.H.; Havekes, L.M.; Groot, P.H.E.

1998-01-01

Apolipoprotein (APO) E*3-Leiden mice with impaired chylomicron and VLDL (very low density lipoprotein) remnant metabolism display hyperlipidaemia and atherosclerosis. In the present study, these mice were used for testing the hypolipidaemic effect of two marketed agents, lovastatin (CAS 75330-75-5)

NMR characterization of foldedness for the production of E3 RING domains

NARCIS (Netherlands)

Huang, A.; de Jong, R.N.; Folkers, G.E.; Boelens, R.

2010-01-01

We summarize the use of NMR spectroscopy in the production and the screening of stability and foldedness of protein domains, and apply it to the RING domains of E3 ubiquitin-ligases. RING domains are involved in specific interactions with E2 ubiquitin-conjugating enzymes and thus play an essential

3rd international KES conference on Smart Education and Smart e-Learning

CERN Document Server

Howlett, Robert; Jain, Lakhmi

2016-01-01

This book contains the contributions presented at the 3rd international KES conference on Smart Education and Smart e-Learning, which took place in Puerto de la Cruz, Tenerife, Spain, June 15-17, 2016. It contains a total of 56 peer-reviewed book chapters that are grouped into several parts: Part 1 - Smart University: Conceptual Modeling, Part 2 – Smart Education: Research and Case Studies, Part 3 – Smart e-Learning, Part 4 – Smart Education: Software and Hardware Systems, and Part 5 – Smart Technology as a Resource to Improve Education and Professional Training. We believe that the book will serve as a useful source of research data and valuable information for faculty, scholars, Ph.D. students, administrators, and practitioners - those who are interested in innovative areas of smart education and smart e-learning. .

Compounding of vitamin A, D3, E and K3 supplements for cystic fibrosis patients: formulation and stability study.

Science.gov (United States)

Huyghebaert, N; De Beer, J; Vervaet, C; Remon, J P

2007-10-01

Cystic fibrosis (CF) patients suffer from malabsorption of fat-soluble vitamins (A, D, E and K). These vitamins are available as water-dispersible (A, D(3) and E) or water-soluble grades (K(3)), which is favoured in CF patients as they fail to absorb oil-based products. The objective of this study was to determine stability of these raw materials after opening the original package and to develop a compounded formulation of acceptable quality, stability and taste, allowing flexible dose adaptation and being appropriate for administration to children and elderly people. The raw materials were stored after opening their original package for 8 months at 8 degrees C and room temperature (RT). Stability was assessed using a validated HPLC method after extraction of the vitamin from the cold water-soluble matrix (vitamin A acetate, D(3) and E) or using a spectrophotometrical method (vitamin K(3)). These materials were mixed with an appropriate lactose grade (lactose 80 m for vitamins A and D(3); lactose 90 m for vitamin E, lactose very fine powder for vitamin K(3)) and filled in hard gelatin capsules. Mass and content uniformity were determined and stability of the vitamins in the capsules was assessed after 2 months storage at 8 degrees C and RT. All raw materials showed good stability during storage in the opened original package for 8 months storage at 8 degrees C as well as RT (>95% of the initial content). The compounded formulations complied with the requirements of the European Pharmacopoeia for mass and content uniformity and can be stored for 2 months at 8 degrees C or RT while maintaining the vitamin content between 90% and 110%. As these fat-soluble vitamins are not commercially available on the Belgian market, compounded formulations are a valuable alternative for prophylactic administration of these vitamins to CF patients, i.e. a stable formulation, having an acceptable taste, allowing flexible dose adaptation and being appropriate for administration to

Glycogen Synthase Kinase 3 (GSK-3) influences epithelial barrier function by regulating Occludin, Claudin-1 and E-cadherin expression

International Nuclear Information System (INIS)

Severson, Eric A.; Kwon, Mike; Hilgarth, Roland S.; Parkos, Charles A.; Nusrat, Asma

2010-01-01

The Apical Junctional Complex (AJC) encompassing the tight junction (TJ) and adherens junction (AJ) plays a pivotal role in regulating epithelial barrier function and epithelial cell proliferative processes through signaling events that remain poorly characterized. A potential regulator of AJC protein expression is Glycogen Synthase Kinase-3 (GSK-3). GSK-3 is a constitutively active kinase that is repressed during epithelial-mesenchymal transition (EMT). In the present study, we report that GSK-3 activity regulates the structure and function of the AJC in polarized model intestinal (SK-CO15) and kidney (Madin-Darby Canine Kidney (MDCK)) epithelial cells. Reduction of GSK-3 activity, either by small molecule inhibitors or siRNA targeting GSK-3 alpha and beta mRNA, resulted in increased permeability to both ions and bulk solutes. Immunofluorescence labeling and immunoblot analyses revealed that the barrier defects correlated with decreased protein expression of AJC transmembrane proteins Occludin, Claudin-1 and E-cadherin without influencing other TJ proteins, Zonula Occludens-1 (ZO-1) and Junctional Adhesion Molecule A (JAM-A). The decrease in Occludin and E-cadherin protein expression correlated with downregulation of the corresponding mRNA levels for these respective proteins following GSK-3 inhibition. These observations implicate an important role of GSK-3 in the regulation of the structure and function of the AJC that is mediated by differential modulation of mRNA transcription of key AJC proteins, Occludin, Claudin-1 and E-cadherin.

Glycogen Synthase Kinase 3 (GSK-3) influences epithelial barrier function by regulating Occludin, Claudin-1 and E-cadherin expression

Energy Technology Data Exchange (ETDEWEB)

Severson, Eric A.; Kwon, Mike; Hilgarth, Roland S.; Parkos, Charles A. [Epithelial Pathobiology Research Unit, Dept. of Pathology, Emory University, Atlanta, GA 30322 (United States); Nusrat, Asma, E-mail: anusrat@emory.edu [Epithelial Pathobiology Research Unit, Dept. of Pathology, Emory University, Atlanta, GA 30322 (United States)

2010-07-02

The Apical Junctional Complex (AJC) encompassing the tight junction (TJ) and adherens junction (AJ) plays a pivotal role in regulating epithelial barrier function and epithelial cell proliferative processes through signaling events that remain poorly characterized. A potential regulator of AJC protein expression is Glycogen Synthase Kinase-3 (GSK-3). GSK-3 is a constitutively active kinase that is repressed during epithelial-mesenchymal transition (EMT). In the present study, we report that GSK-3 activity regulates the structure and function of the AJC in polarized model intestinal (SK-CO15) and kidney (Madin-Darby Canine Kidney (MDCK)) epithelial cells. Reduction of GSK-3 activity, either by small molecule inhibitors or siRNA targeting GSK-3 alpha and beta mRNA, resulted in increased permeability to both ions and bulk solutes. Immunofluorescence labeling and immunoblot analyses revealed that the barrier defects correlated with decreased protein expression of AJC transmembrane proteins Occludin, Claudin-1 and E-cadherin without influencing other TJ proteins, Zonula Occludens-1 (ZO-1) and Junctional Adhesion Molecule A (JAM-A). The decrease in Occludin and E-cadherin protein expression correlated with downregulation of the corresponding mRNA levels for these respective proteins following GSK-3 inhibition. These observations implicate an important role of GSK-3 in the regulation of the structure and function of the AJC that is mediated by differential modulation of mRNA transcription of key AJC proteins, Occludin, Claudin-1 and E-cadherin.

Re-engineering and evaluation of anti-DNA autoantibody 3E10 for therapeutic applications.

Science.gov (United States)

Rattray, Zahra; Dubljevic, Valentina; Rattray, Nicholas J W; Greenwood, Deanne L; Johnson, Caroline H; Campbell, James A; Hansen, James E

2018-02-12

A key challenge in the development of novel chemotherapeutics is the design of molecules capable of selective toxicity to cancer cells. Antibodies have greater target specificity compared to small molecule drugs, but most are unable to penetrate cells, and predominantly target extracellular antigens. A nuclear-penetrating anti-DNA autoantibody isolated from the MRL/lpr lupus mouse model, 3E10, preferentially localizes to tumors, inhibits DNA repair, and selectively kills cancer cells with defects in DNA repair. A murine divalent single chain variable fragment of 3E10 with mutations for improved DNA binding affinity, 3E10 (D31N) di-scFv, has previously been produced in P. pastoris and yielded promising pre-clinical findings, but is unsuitable for clinical testing. The present study reports the design, expression and testing of a panel of humanized 3E10 (D31N) di-scFvs, some of which contain CDR substitution. These variants were expressed in a modified CHO system and evaluated for their physicochemical attributes and ability to penetrate nuclei to selectively cause DNA damage accumulation in and kill cancer cells with DNA repair defects. Secondary structure was conserved and most variants retained the key characteristics of the murine 3E10 (D31N) di-scFv produced in P. pastoris. Moreover, several variants with CDR substitutions outperformed the murine prototype. In conclusion, we have designed several humanized variants of 3E10 (D31N) di-scFv that have potential for application as monotherapy or conjugates for targeted nuclear drug delivery. Copyright © 2018 Elsevier Inc. All rights reserved.

GPM GROUND VALIDATION COMPOSITE SATELLITE OVERPASSES MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validation Composite Satellite Overpasses MC3E dataset provides satellite overpasses from the AQUA satellite during the Midlatitude Continental...

17 CFR 240.15c3-1e - Deductions for market and credit risk for certain brokers or dealers (Appendix E to 17 CFR 240...

Science.gov (United States)

2010-04-01

... credit risk for certain brokers or dealers (Appendix E to 17 CFR 240.15c3-1). 240.15c3-1e Section 240....15c3-1(c)(2)(vi) and (c)(2)(vii) and to compute deductions for credit risk pursuant to this Appendix E... the broker or dealer will use to calculate deductions for market and credit risk on those categories...

Molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in bovine mammary epithelial cells.

Science.gov (United States)

Yu, Cuiping; Luo, Chaochao; Qu, Bo; Khudhair, Nagam; Gu, Xinyu; Zang, Yanli; Wang, Chunmei; Zhang, Na; Li, Qingzhang; Gao, Xuejun

2014-12-15

14-3-3γ, an isoform of the 14-3-3 protein family, was proved to be a positive regulator of mTOR pathway. Here, we analyzed the function of 14-3-3γ in protein synthesis using bovine mammary epithelial cells (BMECs). We found that 14-3-3γ interacted with eIF1AX and RPS7 by 14-3-3γ coimmunoprecipitation (CoIP) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) peptide mass fingerprinting analysis. These interactions of 14-3-3γ with eIF1AX and RPS7 were further confirmed by colocalization and fluorescence resonance energy transfer (FRET) analysis. We also found that methionine could promote protein synthesis and trigger the protein expression levels of 14-3-3γ, eIF1AX and RPS7. Analysis of overexpression and inhibition of 14-3-3γ confirmed that it positively affected the protein expression levels of eIF1AX, RPS7, Stat5 and mTOR pathway to promote protein synthesis and cell proliferation in BMECs. We further showed that overexpression of eIF1AX and RPS7 also triggered protein translation and cell proliferation. From these results, we conclude that molecular network including eIF1AX, RPS7, and 14-3-3γ regulates protein translation and cell proliferation in BMECs. Copyright © 2014 Elsevier Inc. All rights reserved.

Performance Measurement of Advanced Stirling Convertors (ASC-E3)

Science.gov (United States)

Oriti, Salvatore M.

2013-01-01

NASA Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance testing data of the Advanced Stirling Convertor (ASC). The latest version of the ASC (ASC-E3, to represent the third cycle of engineering model test hardware) is of a design identical to the forthcoming flight convertors. For this generation of hardware, a joint Sunpower and GRC effort was initiated to improve and standardize the test support hardware. After this effort was completed, the first pair of ASC-E3 units was produced by Sunpower and then delivered to GRC in December 2012. GRC has begun operation of these units. This process included performance verification, which examined the data from various tests to validate the convertor performance to the product specification. Other tests included detailed performance mapping that encompassed the wide range of operating conditions that will exist during a mission. These convertors were then transferred to Lockheed Martin for controller checkout testing. The results of this latest convertor performance verification activity are summarized here.

Climate Change Communicators: The C3E3 Project

Science.gov (United States)

Sharif, H. O.; Joseph, J.

2013-12-01

The University of Texas at San Antonio (UTSA), San Antonio College (SAC), and the University of North Dakota (UND) have partnered with NASA to provide underrepresented undergraduates from UTSA, SAC, and other community colleges climate-related research and education experiences through the Climate Change Communication: Engineer, Environmental science, and Education (C3E3) project. The program aims to develop a robust response to climate change by providing K-16 climate change education; enhance the effectiveness of K-16 education particularly in engineering and other STEM disciplines by use of new instructional technologies; increase the enrollment in engineering programs and the number of engineering degrees awarded by showing engineering's usefulness in relation to the much-discussed contemporary issue of climate change; increase persistence in STEM degrees by providing student research opportunities; and increase the ethnic diversity of those receiving engineering degrees and help ensure an ethnically diverse response to climate change. Students participated in the second summer internship funded by the project. More than 60 students participated in guided research experiences aligned with NASA Science Plan objectives for climate and Earth system science and the educational objectives of the three institutions. The students went through training in modern media technology (webcasts), and in using this technology to communicate the information on climate change to others, especially high school students, culminating in production of webcasts on investigating the aspects of climate change using NASA data. Content developed is leveraged by NASA Earth observation data and NASA Earth system models and tools. Several departments are involved in the educational program.

Omega Air 707/F/A-18A-D Aerial Refueling Ground and Flight Test Evaluation and MA-3-1 Coupling Ground Test Evaluation

National Research Council Canada - National Science Library

Markowich, Brian

2001-01-01

Omega Air, Incorporated, a private organization that provides shipping and transportation services, contracted Marconi Flight Systems, Incorporated, to retrofit a Boeing 707 aircraft with an internal...

Ácidos graxos ômega 3 e tratamento da esquizofrenia Omega 3 fatty acid and schizophrenia treatment

Directory of Open Access Journals (Sweden)

Juliane Costa Silva Zemdegs

2010-01-01

Full Text Available CONTEXTO: A esquizofrenia é uma desordem psiquiátrica complexa e debilitante cujo tratamento de base é realizado com medicamentos antipsicóticos. No entanto, evidências sugerem que a suplementação dietética com ácidos graxos ômega 3 (n-3 pode ser benéfica em diversas desordens psiquiátricas. OBJETIVO: Revisar a eficácia do n-3 como coadjuvante no tratamento farmacológico da esquizofrenia. MÉTODOS: Realizou-se uma pesquisa nas bases de dados eletrônicas Medline, Lilacs e SciELO. A estratégia de busca também incluiu a busca em árvore. Todos os estudos randomizados e controlados relevantes foram incluídos nesta revisão, independentemente do ano de publicação. RESULTADOS: Até o momento, foram divulgados seis estudos randomizados, duplo-cegos placebo controlados; cinco deles apresentaram resultados positivos na melhora dos sintomas da esquizofrenia, assinalando, ainda, superioridade do ácido graxo eicosapentaenoico (EPA em relação ao ácido graxo docosaexaenoico. Em geral, o consumo de 2 g/dia de EPA conjuntamente com a medicação antipsicótica usual parece reduzir a sintomatologia da esquizofrenia, particularmente os sintomas positivos. CONCLUSÃO: A terapia nutricional com EPA mostrou-se útil como coadjuvante no tratamento da esquizofrenia. Por conseguinte, sugere-se que os pacientes esquizofrênicos sejam encorajados a consumir refeições balanceadas e saudáveis ricas em EPA e, caso a quantidade ideal não seja atingida pela dieta, a suplementação pode ser benéfica.BACKGROUND: Schizophrenia is a complex and debilitating psychiatric disorder, whose primary pharmacological intervention is the use of antipsychotics. There is, however, growing evidence that dietary supplementation with omega 3 fatty acids (n-3 may be beneficial in several psychiatric conditions. OBJECTIVE: To review the efficacy of n-3 as a treatment for schizophrenia. METHODS: Electronic searches of the following databases were performed: Medline

E3 Success Story - Advancing Performance in Sustainability and Workforce Development

Science.gov (United States)

E3: North Carolina advances performance in sustainability and workforce development strategies for the state's manufacturers. The initiative helps communities and manufacturers address energy and sustainability challenges by leveraging expertise.

Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation

Energy Technology Data Exchange (ETDEWEB)

He, Yuan; Wang, Shihui [Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005 (China); Mu, Jing, E-mail: mujing@picb.ac.cn [Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005 (China); Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031 (China); Dai, Lingfeng [Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005 (China); Zhang, Zhong [Department of Stomatology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361005 (China); Sun, Yanan; Shi, Wei [Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005 (China); Ge, Dongtao, E-mail: gedt@xmu.edu.cn [Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005 (China)

2017-02-01

Conducting polymer polypyrrole (PPy) possesses good biocompatibility and conductivity and has been used as functional coatings in bone tissue regeneration. In this study, a cholic acid doped PPy nanowires (PPy NWs) coating was electrochemically polymerized on the surface of titanium (Ti). The porous intertwined PPy NWs coating exhibited excellent electrical conductivity and electrochemical activity, better hydrophilicity and higher surface energy. In vitro cell experiments demonstrated that the PPy NWs coating together with a 10 μA substrate-mediate electrical stimulation (ES) was capable to positive regulate the functions of MC3T3-E1 such as cell adhesion, proliferation and differentiation. Further long-term functions of cell tests including alkaline phosphatase (ALP) activity, bone-carboxyglutamic acid-containing protein (BGP) and calcium deposition were all thoroughly increased. These confirmed that the combination of PPy NWs and ES could accelerate MC3T3-E1 cells mature and osteogenesis. Hence, the PPy NWs coating was an electro bioactive coating and may have potential applications in the treatment of bone damage repairing and regeneration with ES. - Highlights: • The porous PPy nanowire coating was electrochemically polymerized on Ti. • PPy NWs had good electrical conductivity, electrochemical stability and hydrophilicity. • Pure PPy NWs with ES could obviously promote the growth and cell functions of MC3T3-E1.

Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation

International Nuclear Information System (INIS)

He, Yuan; Wang, Shihui; Mu, Jing; Dai, Lingfeng; Zhang, Zhong; Sun, Yanan; Shi, Wei; Ge, Dongtao

2017-01-01

Conducting polymer polypyrrole (PPy) possesses good biocompatibility and conductivity and has been used as functional coatings in bone tissue regeneration. In this study, a cholic acid doped PPy nanowires (PPy NWs) coating was electrochemically polymerized on the surface of titanium (Ti). The porous intertwined PPy NWs coating exhibited excellent electrical conductivity and electrochemical activity, better hydrophilicity and higher surface energy. In vitro cell experiments demonstrated that the PPy NWs coating together with a 10 μA substrate-mediate electrical stimulation (ES) was capable to positive regulate the functions of MC3T3-E1 such as cell adhesion, proliferation and differentiation. Further long-term functions of cell tests including alkaline phosphatase (ALP) activity, bone-carboxyglutamic acid-containing protein (BGP) and calcium deposition were all thoroughly increased. These confirmed that the combination of PPy NWs and ES could accelerate MC3T3-E1 cells mature and osteogenesis. Hence, the PPy NWs coating was an electro bioactive coating and may have potential applications in the treatment of bone damage repairing and regeneration with ES. - Highlights: • The porous PPy nanowire coating was electrochemically polymerized on Ti. • PPy NWs had good electrical conductivity, electrochemical stability and hydrophilicity. • Pure PPy NWs with ES could obviously promote the growth and cell functions of MC3T3-E1.

Influence of MC3T3-E1 preosteoblast culture on the corrosion of a T6-treated AZ91 alloy.

Science.gov (United States)

Brooks, Emily K; Tobias, Menachem E; Yang, Shuying; Bone, Lawrence B; Ehrensberger, Mark T

2016-02-01

This study investigated the corrosion of artificially aged T6 heat-treated Mg-9%Al-1%Zn (AZ91) for biomedical applications. Corrosion tests and surface analysis were completed both with and without a monolayer of mouse preosteoblast MC3T3-E1 cells cultured on the sample. Electrochemical impedance spectroscopy (EIS) and inductively coupled plasma mass spectroscopy (ICPMS) were used to explore the corrosion processes after either 3 or 21 days of AZ91 incubation in cell culture medium (CCM). The EIS showed both the inner layer resistance (Rin ) and outer layer resistance (Rout ) were lower for samples without cells cultured on the surface at 3 days (Rin  = 2.64 e4 Ω/cm(2) , Rout  = 140 Ω/cm(2) ) compared to 21 days (Rin  = 3.60 e4 Ω/cm(2) , Rout  = 287 Ω/cm(2) ) due to precipitation of magnesium and calcium phosphates over time. Samples with preosteoblasts cultured on the surface had a slower initial corrosion (3 day, Rin  = 1.88 e5 Ω/cm(2) , Rout  = 1060 Ω/cm(2) ) which was observed to increase over time (21 day, Rin  = 2.99 e4 Ω/cm(2) , Rout  = 287 Ω/cm(2) ). Changes in the corrosion processes were thought to be related to changes in the coverage provided by the cell layer. Our results reveal that the presence of cells and biological processes are able to significantly influence the corrosion rate of AZ91. © 2015 Wiley Periodicals, Inc.

Measurement of e-p→e-X differential cross sections at high Q2 and of the structure function xF3 with ZEUS at HERA

International Nuclear Information System (INIS)

Kappes, A.

2001-12-01

The 16 pb -1 of e - p data taken with the ZEUS detector during the 1998/99 running period of HERA have been used to measure neutral current single- and double-differential cross sections in deep inelastic e - p scattering in the range 200 GeV 2 2 2 and 3.7.10 -3 2 is visible. A comparison of the measured e - p cross sections to those from e + p clearly reveals the influence of the Z-boson contribution for Q 2 >or∼ M Z 2 . This dependence is exploited to determine the mass of the Z boson, M Z , and by combining the e - p and e + p cross sections the parity violating structure function xF 3 is extracted for the first time in deep-inelastic ep scattering at ZEUS. (orig.)

Lunar Penetrating Radar onboard the Chang'e-3 mission

Science.gov (United States)

Fang, Guang-You; Zhou, Bin; Ji, Yi-Cai; Zhang, Qun-Ying; Shen, Shao-Xiang; Li, Yu-Xi; Guan, Hong-Fei; Tang, Chuan-Jun; Gao, Yun-Ze; Lu, Wei; Ye, Sheng-Bo; Han, Hai-Dong; Zheng, Jin; Wang, Shu-Zhi

2014-12-01

Lunar Penetrating Radar (LPR) is one of the important scientific instruments onboard the Chang'e-3 spacecraft. Its scientific goals are the mapping of lunar regolith and detection of subsurface geologic structures. This paper describes the goals of the mission, as well as the basic principles, design, composition and achievements of the LPR. Finally, experiments on a glacier and the lunar surface are analyzed.

Simple synthesis of pyrrolo[3,2-e]indole-1-carbonitriles

Directory of Open Access Journals (Sweden)

Adam Trawczyński

2013-05-01

Full Text Available Alkylation of 5-nitroindol-4-ylacetonitriles with ethyl chloroacetate, α-halomethyl ketones, and chloroacetonitrile followed by a treatment of the products with chlorotrimethylsilane in the presence of DBU gives 1-cyanopyrrolo[3,2-e]indoles substituted in position 2 with electron-withdrawing groups.

(2E-3-(3,5-Dimethyl-1-phenyl-1H-pyrazol-4-yl-1-(2,5-dimethyl-3-thienylprop-2-en-1-one

Directory of Open Access Journals (Sweden)

Salman A. Khan

2010-04-01

Full Text Available The title compound, (2E-3-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl-1-(2,5-dimethyl-3-thienylprop-2-en-1-one (3 was synthesized in high yield by aldol condensation of 3-acetyl-2,5-dimethylthiophene and 3,5-dimethyl-1-phenylpyrazole-4-carboxaldehyde in ethanolic NaOH at room temperature. Its structure was fully characterized by elemental analysis, IR, 1H NMR, 13C NMR and EI-MS spectral analysis.

GPM GROUND VALIDATION SATELLITE SIMULATED ORBITS MC3E V1

Data.gov (United States)

National Aeronautics and Space Administration — The GPM Ground Validation Satellite Simulated Orbits MC3E dataset is available in the Orbital database , which takes account for the atmospheric profiles, the...

The Army War College Review. Volume 2, Number 3, August 2016. Student Publications

Science.gov (United States)

2016-08-01

natohq/opinions_128311.htm?selectedLocale=en. 99 President Schulz: We Need a Common Foreign and Security Policy (Washington, DC: HT Media Ltd, March...cause injury or death to persons or damage or destruction to objects.”13 The data stolen from defense contractors Boeing and Lockheed-Martin by Chinese